gebhart/patrepeval huggingface dataset:
Study of Bone Marrow and Embryonic Stem Cell-Derived Human Mesenchymal Stem Cells for Treatment ofEscherichia coliEndotoxin-Induced Acute Lung Injury in Mice (2015)

To determine the optimal source of mesenchymal stem cells (MSCs) for cell-based therapy for acute lung injury, bone marrow (BM)- and embryonic stem cell-derived human MSC (ES-MSCs) were compared as therapeutic agents for Escherichia coli endotoxin-induced lung injury in mice. ES-MSCs behaved similarly to BM-MSCs by markedly decreasing the inflammatory response induced by endotoxin. However, unlike BM-MSCs, ES-MSCs provided no protective effects against increasing lung water and protein permeability, in part because of an increase in expression of matrix metallopeptidase 9 by ES-MSCs. In patients with acute respiratory distress syndrome, impaired alveolar fluid clearance (i.e., no resolution of pulmonary edema fluid) has been associated with higher mortality rates. Although ES-MSCs might ultimately be found to have properties superior to those of BM-MSCs, such as for immunomodulation, these results highlight the need for a disease-specific potency assay for stem cell-based therapy.
Los principales lineamientos para una política de población en América Latina. Propuestas de las Naciones Unidas para el período 1960-1980 (2002)

El presente ensayo es un recuento parcial de cuáles han sido las recomendaciones formuladas por la Organización de las Naciones Unidas para la aplicación de una política de población para América Latina, durante el período1960-1980. Para ello se hace necesario aproximarse a los lineamientos básicos que fundamentan tales recomendaciones, a fin de obtener una visión ge-neral de la postura asumida por las Naciones Unidas en torno a las políticas de población para el continente suramericano en el contexto internacional. En particular, se observa la relación que se estableció entre población y desarrollo para la época desde la perspectiva de las Naciones Unidas y se discute cuáles han sido los principales obstáculos que se han presentado en el transcurso de la elaboración de la misma, tomando en consideración las comparaciones emiti-das por el sistema de la ONU para concluir algunos comentarios críticos.
Extracting and Exploring the Geo-Temporal Semantics of Textual Resources (2008)

Geo-temporal criteria are important for filtering, grouping and prioritizing information resources. This presents techniques for extracting semantic geo-temporal information from text, using simple text mining methods that leverage on a gazetteer. A prototype system, implementing the proposed methods and capable of displaying information over maps and timelines, is described. This prototype can take input in RSS, demonstrating the application to content from many different online sources. Experimental results demonstrate the efficiency and accuracy of the proposed approaches.
Mixed-component metal–organic frameworks (MC-MOFs): enhancing functionality through solid solution formation and surface modifications (2011)

Mixed-component metal–organic frameworks (MC-MOFs) are metal–organic frameworks that have different linkers or metals with the same structural role. Many of these mixed-ligand or mixed-metal MOFs are solid solutions, in which the proportions of the ligands or metals can be adjusted or even controlled. These MC-MOFs can be prepared directly, using more than one metal or ligand in the synthesis, or formed by post-synthetic modification. A second class of MC-MOFs have core–shell structures, and these can be prepared through epitaxial growth of one MOF on the surface of another or post-synthetic modification of the crystal surfaces. This review describes the syntheses, structures and properties of mixed-ligand, mixed-metal and core–shell MOFs, and highlights some of the potential benefits in functionality that these materials have.
Lentiviral Vectors: Their Molecular Design, Safety, and Use in Laboratory and Preclinical Research (2011)

Lentiviral vectors have been successfully used in the clinic and they are increasingly being used for nonclinical applications. They are capable of stably transducing a broad range of mammalian cell types, including nondividing cells, with high efficiency. This review summarizes the evolving molecular design of lentiviral vectors, describing how they have improved since their first description. Lentiviral vector safety and issues surrounding genotoxicity are discussed. Examples of successful application of lentiviral vectors in laboratory and preclinical research are described. These include functional genomics, target validation, protein manufacturing, in vivo imaging, transgenic animals, and stem cell research.
Phosphorylation and activation of cAMP-dependent protein kinase by phosphoinositide-dependent protein kinase (1998)

Although phosphorylation of Thr-197 in the activation loop of the catalytic subunit of cAMP-dependent protein kinase (PKA) is an essential step for its proper biological function, the kinase responsible for this reaction in vivo has remained elusive. Using nonphosphorylated recombinant catalytic subunit as a substrate, we have shown that the phosphoinositide-dependent protein kinase, PDK1, expressed in 293 cells, phosphorylates and activates the catalytic subunit of PKA. The phosphorylation of PKA by PDK1 is rapid and is insensitive to PKI, the highly specific heat-stable protein kinase inhibitor. A mutant form of the catalytic subunit where Thr-197 was replaced with Asp was not a substrate for PDK1. In addition, phosphorylation of the catalytic subunit can be monitored immunochemically by using antibodies that recognize Thr-197 phosphorylated enzyme but not unphosphorylated enzyme or the Thr197Asp mutant. PDK1, or one of its homologs, is thus a likely candidate for the in vivo PKA kinase that phosphorylates Thr-197. This finding opens a new dimension in our thinking about this ubiquitous protein kinase and how it is regulated in the cell.
Juvenile Salmon Acoustic Telemetry System Transmitter Downsize Assessment (2010)

At the request of the U.S. Army Corps of Engineers, Portland District, researchers from Pacific Northwest National Laboratory investigated the use of an application-specific integrated circuit (ASIC) to reduce the weight and volume of Juvenile Salmon Acoustic Telemetry System (JSATS) transmitters while retaining current functionality. Review of the design of current JSATS transmitters identified components that could be replaced by an ASIC while retaining the function of the current transmitter and offering opportunities to extend function if desired. ASIC design alternatives were identified that could meet transmitter weight and volume targets of 200 mg and 100 mm3. If alternatives to the cylindrical batteries used in current JSATS transmitters can be identified, it could be possible to implant ASIC-based JSATS transmitters by injection rather than surgery. Using criteria for the size of fish suitable for surgical implantation of current JSATS transmitters, it was concluded that fish as small as 70 mm in length could be implanted with an ASIC-based transmitter, particularly if implantation by injection became feasible.
An Arabidopsis Mutant with Enhanced Resistance to Powdery Mildew (1998)

We have identified an Arabidopsis mutant that displays enhanced disease resistance to the fungus Erysiphe cichoracearum, causal agent of powdery mildew. The edr1 mutant does not constitutively express the pathogenesis-related genes PR-1, BGL2, or PR-5 and thus differs from previously described disease-resistant mutants of Arabidopsis. E. cichoracearum conidia (asexual spores) germinated normally and formed extensive hyphae on edr1 plants, indicating that the initial stages of infection were not inhibited. Production of conidiophores on edr1 plants, however, was <16% of that observed on wild-type Arabidopsis. Reduction in sporulation correlated with a more rapid induction of defense responses. Autofluorescent compounds and callose accumulated in edr1 leaves 3 days after inoculation with E. cichoracearum, and dead mesophyll cells accumulated in edr1 leaves starting 5 days after inoculation. Macroscopic patches of dead cells appeared 6 days after inoculation. This resistance phenotype is similar to that conferred by "late-acting" powdery mildew resistance genes of wheat and barley. The edr1 mutation is recessive and maps to chromosome 1 between molecular markers ATEAT1 and NCC1. We speculate that the edr1 mutation derepresses multiple defense responses, making them more easily induced by virulent pathogens.
New Lookup Tables and Searching Algorithms for Fast H.264/AVC CAVLC Decoding (2010)

In this paper, new codeword structures, tables, and searching methods for fast and efficient <emphasis emphasistype="italic" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">coeff_token</emphasis> , <emphasis emphasistype="italic" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">total_zeros</emphasis> , and <emphasis emphasistype="italic" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">run_before</emphasis> decoding are developed. This new achievement is mainly based on the fact that the context-adaptive variable length coding (CAVLC) decoding can be modeled as a finite state machine. In order to quantitatively evaluate the proposed method in terms of decoding speed and complexity, we define the iteration bound <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$\left({{1}\over {\mathtilde{\tau}}}\right)$</tex></formula> and the <emphasis emphasistype="italic" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">complexity ratio</emphasis> <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$(CR)$</tex></formula> . Using these gauge variables, we show that the new algorithms reduce <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">${\mathtilde {\tau}}$</tex></formula> to about one third and <emphasis emphasistype="italic" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">complexity ratio</emphasis> to 0.95. This means that the proposed techniques reduce the decoding time to about one third and memory access count by 90% compared to those of the conventional methods without implementation overheads. Multiple-symbol parallel decoding method for <emphasis emphasistype="italic" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">run_before</emphasis> syntax element is proposed based on a <emphasis emphasistype="italic" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">bit-positioning</emphasis> with the critical path latency of only one multiplexer for the post-combination process. The proposed methods make it possible to implement a fast and efficient CAVLC decoding without losing video quality on any environments.
Linking Reservoir and Surface Simulators: How to Improve the Coupled Solutions (2000)

Abstract A full analysis of the problem of coupling two independent industrial simulators, a reservoir simulator and a steady-state network simulator, has been performed. A classification of the industrial needs has been obtained from the examination of several actual cases studies (fields at development, or production stage, requiring performance optimization through short to long term forecasts). Several coupling configurations and specific requirements, for both reservoir and surface software, have been identified to satisfy the industrial needs. Various commercial solutions have been screened to evaluate the adequacy between the software functional capacities and the identified configurations and requirements. Limitations have been brought to evidence. The paper, issued from the work of a multidisciplinary team, presents the details of each step described briefly here above. Practical solutions for improving coupled solutions, i.e. to go beyond the identified limitations and to reduce the elapsed times of the coupled simulations, are provided. An improved mutual understanding of the needs of each discipline concerned by the integration of reservoir and surface has been experienced as essential for a successful integrated approach of field optimization. It is intended in the paper to contribute to a better communication between the various upstream petroleum skills: reservoir, production, surface and process engineers.
p-Hydroxyphenylacetaldehyde, an Aldehyde Generated by Myeloperoxidase, Modifies Phospholipid Amino Groups of Low Density Lipoprotein in Human Atherosclerotic Intima (2000)

Oxidation of low density lipoprotein (LDL) may be of critical importance in the pathogenesis of atherosclerosis. Recent studies suggest that oxidized phospholipids render LDL atherogenic. However, both the structures and the physiologically relevant pathways for the formation of modified phospholipids in oxidized LDL remain poorly understood. We previously showed that p-hydroxyphenylacetaldehyde (pHA) is the major product of L-tyrosine oxidation by the myeloperoxidase/hydrogen peroxide/chloride system of phagocytes. In the current studies, we demonstrate that this reactive aldehyde targets the aminophospholipids of LDL in vitro and in vivo. Activated human neutrophils generated pHA-ethanolamine, the reduced adduct of pHA with the amino group of phosphatidylethanolamine, on LDL phospholipids by a reaction that required myeloperoxidase, H(2)O(2), and L-tyrosine. The cellular system could be replaced by HOCl and L-tyrosine but not by a wide variety of other oxidation systems, indicating that pHA-ethanolamine is a specific marker for covalent modification of aminophospholipids by myeloperoxidase. To determine whether aldehydes modify aminophospholipids in vivo, we quantified levels of pHA-ethanolamine in acid hydrolysates of reduced lipid extracts through isotope dilution gas chromatography/mass spectrometry. Circulating LDL contained undetectable levels of pHA-modified phospholipid (<0.1 mmol/mol). In contrast, the concentration of pHA-ethanolamine in LDL isolated from human atherosclerotic lesions was strikingly elevated (4.5 mmol/mol). Collectively, these results demonstrate a novel, myeloperoxidase-based mechanism for modifying the amino group of LDL phospholipids. They also offer the first evidence that myeloperoxidase may damage LDL lipids in vivo, raising the possibility that aldehyde-modified aminophospholipids play a role in inflammation and vascular disease.
Online index rebuild (2000)

In this paper we present an efficient method to do online rebuild of a B+-tree index. This method has been implemented in Sybase Adaptive Server Enterprise (ASE) Version 12.0. It provides high concurrency, does minimal amount of logging, has good performance and does not deadlock with other index operations. It copies the index rows to newly allocated pages in the key order so that good space utilization and clustering are achieved. The old pages are deallocated during the process. Our algorithm differs from the previously published online index rebuild algorithms in two ways. It rebuilds multiple leaf pages and then propagates the changes to higher levels. Also, while propagating the leaf level changes to higher levels, level 1 1 pages are reorganized, eliminating the need for a separate pass. Our performance study shows that our approach results in significant reduction in logging and CPU time. Also, our approach uses the same concurrency control mechanism as split and shrink operations, which made it attractive for implementation.
Expression Analysis of Recombinant Lysyl Oxidase (LOX) in Myofibroblastlike Cells (2002)

Lysyl oxidase (LOX), originally known as the enzyme required for initiation of covalent cross-linking in collagens and elastin, is now known to be a member of a family of genetically related proteins. LOX, or a related protein, has also been localized intracellularly, both in association with the cytoskeleton and in the cell nucleus. To determine the structural requirements for secretion, maturation, and nuclear location of LOX in a cellular context, we have devised an homologous cell model for expression of the recombinant protein. Murine recombinant LOX was expressed in 3T6-5 myofibroblast-like cells as a 51-kD precursor, which was observed in the cytoplasm but not in the nucleus. To investigate whether potential alternative translation initiation sites were involved in specifying a nuclear form of LOX, constructs mutated or deleted for ATG +1 were used, but alternative initiation at CTG &#109 315 or ATG +418 did not lead to the expression of intranuclear forms. Residues 23 to 157 of the proregion were essential for export of the precursor, while mutation of the putative site for maturation by procollagen C-proteinase abolished processing to the mature form of the enzyme. Cross-linking of collagen, as measured by pyridinoline analysis, increased twofold with the recombinant cells, compared to non-transfected controls. This shows the specific contribution of LOX, as opposed to other genetic forms of the enzyme, to cross-linking in a cellular context.
Laser-Induced Thermal Damage of Skin (1977)

Abstract : A computerized model was developed for predicting thermal damage of skin by laser exposures. THERMAL, OPTICAL, AND PHYSIOLOGICAL DATA ARE PRESENTED FOR THE MODEL. Model predictions of extent of irreversible damage were compared with histologic determinations of the extent of damage produced in pig skin by carbon dioxide and ruby lasers. (Author)
Gene expression, immunolocalization, and secretion of human defensin-5 in human female reproductive tract. (1998)

This study describes the novel localization of the antimicrobial peptide human intestinal defensin-5 (HD-5) in female genital tract epithelia. Using a 3' rapid amplification of cDNA ends (RACE) protocol, HD-5 was cloned from a vaginal epithelial cell RNA preparation, and its identity was confirmed by sequencing. Tissue samples from multiple donors were subsequently screened for HD-5 expression by reverse transcription polymerase chain reaction. HD-5 message was invariantly expressed by normal vagina and ectocervix and inflamed fallopian tube, but variably expressed by normal endocervix, endometrium, and fallopian tube (60, 64, and 29% of specimens, respectively). Expression in endometrium was the highest during the early secretory phase of the menstrual cycle. Using immunohistochemistry and confocal microscopy, HD-5 peptide was localized in the upper half of the stratified squamous epithelium of the vagina and ectocervix, with the intensity of cellular staining increasing toward the lumen. In positive endocervix, endometrium, and fallopian tube specimens, HD-5 was located in apically oriented granules and on the apical surface of a proportion of columnar epithelial cells. Using Western blot analysis, secreted HD-5 was detected in cervicovaginal lavages, with the highest concentrations found during the secretory phase of the menstrual cycle. We hypothesize that HD-5 is an intrinsic component of the female urogenital innate immune defense system and that its expression may be modulated by hormonal and proinflammatory factors.
Oxidative Stress in Cancer, AIDS, and Neurodegenerative Diseases (1997)

Oxidative stress in cell biology and biochemistry oxidative stress and cellular signalling oxidative stress and apoptosis oxidative stress and antioxidants oxidative stress and cancer oxidative stress and AIDS oxidative stress and neurodegenerative diseases and other pathologies. The editors of this work are three of the most respected pioneers in the field. Dr. Montagnier is credited as the discoverer of HIV.
Detection of the gene for toxic shock syndrome toxin 1 in Staphylococcus aureus by enzyme-labelled oligonucleotide probes (1996)

A colony hybridisation method with enzyme-labelled oligonucleotide probes was developed to detect the gene for toxic shock syndrome toxin 1 (tst). For rapid identification, bacterial colonies were transferred from agar plates directly on to nylon membranes. These procedures took only 3 h. Results obtained by this test correlated well with those obtained by the reverse passive latex agglutination test. Thus, this method is convenient and reliable for the detection of tst in staphylococci, which could be useful for both research and clinical purposes. This method demonstrated that tst was more prevalent in methicillin-resistant Staphylococcus aureus (56%) than in methicillin-susceptible S. aureus (4%).
<i>Hes1 </i>and <i>Hes5</i> Regulate the Development of the Cranial and Spinal Nerve Systems (2006)

The basic helix-loop-helix genes Hes1 and Hes5, known Notch effectors, regulate the maintenance of neural stem cells and the development of the central nervous system (CNS). In the absence of Hes1 and Hes5, the size, shape and cytoarchitecture of the CNS are severely disorganized, but the development of the peripheral nervous system remains to be analyzed. Here, we found that in Hes1;Hes5 double-mutant mice, the cranial and spinal nerve systems are also severely disorganized. In these mutant mice, axonal projections from the mesencephalic neurons to the trigeminal (V) ganglion become aberrant and the proximal parts of the glossopharyngeal (IX) and vagus (X) nerves are fused. The hypoglossal (XII) nerve is also formed poorly. Furthermore, the dorsal root ganglia are fused with the spinal cord, and the dorsal and ventral roots of the spinal nerves are lacking in many segments. These results indicate that Hes1 and Hes5 play an important role in the formation of the cranial and spinal nerve systems.
In vivo electrotransfer of the cardiotrophin-1 gene into skeletal muscle slows down progression of motor neuron degeneration in pmn mice (2002)

Among all vectors designed for gene therapy purposes, adenovirus appears to be the most efficient in vivo vehicle to transduce the broadest spectrum of cellular targets. However, the deleterious immunogenicity of this viral vector impedes its use in chronic diseases. Non-viral vectors, such as naked DNA, are attractive alternatives for safety and technical issues, such as scale-up production. Naked DNA injection, greatly improved when combined with electroporation, showed great potential in adult animals, especially when directed to the muscle. We have recently proven the therapeutic effect of a neonatal single intramuscular injection of a cardiotrophin-1 (CT-1)-encoding adenovirus in a hereditary disease mouse model of human motor neuron disease, the progressive motor neuronopathy (pmn) mutant. We now demonstrate that a single injection/electroporation of a CT-1-encoding plasmid in neonate pmn mice is almost as efficient as adenovirus-mediated gene transfer with respect to survival, muscular function and neuroprotection of the animals. Treated mice gain global weight, their mean lifespan is extended by 25%, all their electromyographic parameters are improved and myelinated axons of their phrenic nerves are protected. Moreover, we show that re-injection/electroporation leads to improvements in this neuroprotection. We therefore demonstrate for the first time the therapeutic efficacy of neonatal intramuscular DNA injection/electroporation in a murine model of a human hereditary disorder.