gebhart/patrepeval huggingface dataset:
A Study of the Partitioning of Colloidal Particles Based on Their Size during Electrostatic Immobilization at the Air−Water Interface Using Fatty Amine Monolayers (1997)

We have recently demonstrated that carboxylic acid derivatized silver and gold colloidal particles can be immobilized through electrostatic interaction with fatty amine Langmuir monolayers at the air−water interface and that good quality multilayer films of the clusters can be deposited by the Langmuir−Blodgett (LB) technique. In this paper, we extend our study on the electrostically controlled immobilization process through an investigation of the partitioning of carboxylic acid derivatized clusters based on their size. Simultaneous immobilization of gold and silver clusters (130 ± 30 and 70 ± 12 Å diameter, respectively) from the same colloidal subphase at the air−water interface using octadecylamine Langmuir monolayers has been followed by π−A isotherm measurements as well as optical absorption, quartz crystal microgravimetry (QCM), and X-ray fluorescence (XRF) analysis of the built-up LB films. Even for small concentrations of gold relative to silver clusters in the subphase, the density of the larger gold clusters in the LB films was found to be surprisingly high in relation to the smaller silver clusters. While the π−A isotherms stabilized within 3 h of spreading the fatty amine monolayer, the cluster density at the air−water interface continued to increase, taking up to 12 h to reach equilibrium. The larger gold clusters reached an equilibrium density at the interface much faster than the smaller silver clusters. The “reverse” fractionation of clusters observed is discussed in terms of an electrostatic model.
Zero-Emission MATIANT Cycle (1999)

In this paper, a novel technology based on the zero CO2 emission MATIANT (contraction of the names of the two designers MAThieu and IANTovski) cycle is presented. This latter is basically a gas cycle and consists of a supercritical CO2 Rankine-like cycle on top of regenerative CO2 Brayton cycle. CO2 is the working fluid and O2 is the fuel oxidizer in the combustion chambers. The cycle uses the highest temperatures and pressures compatible with the most advanced materials in the steam and gas turbines. In addition, a reheat and a staged compression with intercooling are used. Therefore, the optimized cycle efficiency rises up to around 45 percent when operating on natural gas. A big asset of the system is its ability to remove the CO2 produced in the combustion process in liquid state and at high pressure, making it ready for transportation, for reuse or for final storage. The assets of the cycle are mentioned. The technical issues for the predesign of a prototype plant are reviewed.
Chromatin Remodeling Is Required for Gene Reactivation after Decitabine-Mediated DNA Hypomethylation (2010)

The DNA hypomethylating drug decitabine (DAC) reactivates silenced gene expression in cancer and is approved for the treatment of the myelodysplastic syndrome. Gene reactivation after DAC is variable and incompletely understood. Here, we established a cell line system (YB5) derived from the SW48 colon cancer cell line to study DAC-induced reactivation. YB5 contains a hypermethylated cytomegalovirus promoter driving green fluorescent protein (GFP), and the locus is transcriptionally silent. GFP reexpression can be achieved by DAC treatment, but the expression level of individual cells is heterogeneous. DAC-treated YB5 cells were separated into GFP-positive and GFP-negative subpopulations. By comparing DAC-treated sorted GFP-positive and GFP-negative cells, we found that their methylation levels were similarly decreased but that histone modifications and histone H3 densities were remarkably different. Despite a similar degree of (incomplete) DNA hypomethylation, GFP-positive cells reverted to an active chromatin structure marked by higher H3K9 acetylation, lower H3K27 trimethylation, and lower promoter nucleosome density. GFP-negative cells had histone modifications and promoter nucleosome density, similar to parental cells. On DAC withdrawal, gradual resilencing and remethylation occurred in both GFP-positive and GFP-negative cells, and the resilencing correlated with a gradual increase in nucleosome occupancy in GFP-positive cells. These data show that hypomethylation alone after DAC is insufficient for gene expression induction, and that chromatin resetting to an active state including nucleosome eviction is required for activation of protein expression. Our findings suggest that gene expression is the key in optimizing DAC treatment strategies in the clinic.
The role of serotonin in tumour growth (Review) (2005)

Serotonin (5-hydroxytryptamine; 5HT) a monoamine neurotransmitter mediates a wide range of physiological actions in the human body. For example 5HT is implicated in psychiatric and neurological disorders and also plays a fundamental role in tumour growth, differentiation and gene expression. 5HT acts as a growth factor for several types of tumoural and non-tumoural cells. This review considers the role of 5HT and its receptors in the human body with particular reference to carcinogenesis. We conclude that 5HT causes growth proliferation and 5HT antagonists cause growth inhibition in a variety of tumour cells (e.g. prostate carcinoma, lung carcinoma and colonic carcinoma). Therefore, further studies should look into the potential use of 5HT antagonists in the treatment of cancer.
Role of Endogenous Primate LH in Maintaining Corpus Luteum Function of the Monkey (1972)

ABSTRACT Antiserum to human chorionic gonadotropin (Ayerst) was prepared by administering it in Freund's complete adjuvant to rabbits. Contaminating non-specific antibodies were absorbed with dilute normal human serum and a kaolin-acetone extract prepared from urine of children. Cross-reactivity of this material with human LH or monkey pituitary extract was established using the Ouchterlony double diffusion and competitive binding test. Biological testing involved the capacity of this material to neutralize the ovulation inducing activity of human LH and monkey pituitary extract. It was also able to compete with these two preparations in a modified radioimmunoassay using 125I-labeled human LH. This LH specific antibody (CG A/S) was then injected (2 ml/day) on days 15 through 18 or 15 and 16 into normally cycling Macaca jascicularis. Blood samples were drawn on alternate days beginning on day 9 during the experiment and the serum assayed for progesterone. Progesterone values increased due to ovulation and ...
Preclinical and clinical development of immunocytokines. (2003)

Advances in preclinical and clinical development have demonstrated that monoclonal antibodies and immuno-activating cytokines have a beneficial role in certain clinical oncology settings. Genetic engineering has now been used to create 'immunocytokines (ICs)'. These are fusion proteins that consist of an immune-activating cytokine linked to a tumor-reactive monoclonal antibody. Preclinical data demonstrate that ICs are far more effective in murine tumor models than the separate molecules from which they are derived. Clinical testing of ICs has recently begun using an anti-GD2 monoclonal antibody linked to interleukin-2 (IL-2) (hu14.18-IL-2), and using an antibody directed against the human epithelial cell adhesion molecule linked to IL-2 (KS-IL-2).
Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties. (1987)

This study examines the osteoblastic properties of the established human osteosarcoma cell line Saos-2. Saos-2 cells inoculated into diffusion chambers, which were implanted i.p. into nude mice, produced mineralized matrix in 4 of 6 chambers at 8 weeks. In 5 of 6 chambers there was a strong positive alkaline phosphatase reaction. In culture the alkaline phosphatase levels increased with time and cell density, reaching very high levels at confluence: 4-7 mumol/mg protein/min. The cells show a sensitive adenylate cyclase response to parathyroid hormone, 50% effective dose = 2.8 nM, which increases with cell density and is further raised by dexamethasone treatment. They also exhibit typical binding of 1-25-dihydroxyvitamin D3 to 3.2S receptor protein with an apparent Kd of 0.21 nM; the numbers of sites per cell were 3,300 at 50,000 cells/cm2 and 1,800 at 280,000 cells/cm2. The presence of osteonectin was visualized with a monoclonal antibody which revealed a reticular pattern on the cell surface. Osteonectin was also detected in the medium by Western blots, migrating at around Mr 40,000 in nonreduced gels and Mr 44,000 in reduced gels. The Saos-2 cells thus possess several osteoblastic features and could be useful as a permanent line of human osteoblast-like cells and as a source of bone-related molecules.
Disulfide Linkages in a Heat-Stable Enterotoxin (STp) Produced by a Porcine Strain of Enterotoxigenic Escherichia coli (1988)

Abstract Heat-stable enterotoxins, produced by various enteric bacteria such as enterotoxigenic Escherichia coli, Yersinia enterocolitica, and Vibrio cholerae non-01, have common segments consisting of 13 amino acid residues (T. Takao et al., FEBS Lett., 193, 250 (1985)). These segments have full enterotoxigenic activity and are bound by three intramolecular disulfide bonds. The linkages of these three disulfide bonds in a heat-stable enterotoxin (STp) produced by a porcine strain of enterotoxigenic E. coli were determined to be (Remark: Graphics omitted.) by stepwise and selective formation of disulfide bonds using different types of removable protecting groups for Cys residues. These disulfide linkages were identical with those of a heat-stable enterotoxin (STh) produced by a human strain of enterotoxigenic E. coli (Y. Shimonishi et al., FEBS Lett., 215, 165 (1987)).
Linking genes to literature: text mining, information extraction, and retrieval applications for biology (2008)

Efficient access to information contained in online scientific literature collections is essential for life science research, playing a crucial role from the initial stage of experiment planning to the final interpretation and communication of the results. The biological literature also constitutes the main information source for manual literature curation used by expert-curated databases. Following the increasing popularity of web-based applications for analyzing biological data, new text-mining and information extraction strategies are being implemented. These systems exploit existing regularities in natural language to extract biologically relevant information from electronic texts automatically. The aim of the BioCreative challenge is to promote the development of such tools and to provide insight into their performance. This review presents a general introduction to the main characteristics and applications of currently available text-mining systems for life sciences in terms of the following: the type of biological information demands being addressed; the level of information granularity of both user queries and results; and the features and methods commonly exploited by these applications. The current trend in biomedical text mining points toward an increasing diversification in terms of application types and techniques, together with integration of domain-specific resources such as ontologies. Additional descriptions of some of the systems discussed here are available on the internet http://zope.bioinfo.cnio.es/bionlp_tools/.
A high‐throughput model for fat graft assessment (2009)

This model of fat-grafting and early apoptosis can be used to screen agents and grafting methods and predict long-term graft survival. We show that levels of apoptosis within ten days correlate with weight, DNA, and histology, at six weeks. Using this model, long-term adipocyte survival and graft take can be predicted during the first 10 days post-implantation.
Assessing stability‐indicating methods for coelution by two‐dimensional liquid chromatography with mass spectrometric detection (2014)

Chromatographic analysis of trace organic impurities/degradants coeluting in the midst of active pharmaceutical ingredient can be challenging given similarities in their structures and differences in their relative levels/intensities. Conventional detection techniques such as diode array detection and mass spectrometry are often inadequate to detect/identify these residual coeluting impurities and could result in a false negative. Application of two-dimensional chromatography to address/evaluate coelution in conventional chromatography is presented. Areas of interest, usually corresponding to the main component, are transferred to secondary column/s for further separation termed as pseudocomprehensive two-dimensional liquid chromatography. Coelution, if any, in the rest of the chromatogram is monitored using conventional detectors. In this work, the use of similar and complementary phases in both dimensions is presented. The use of the same phase in both dimensions to resolve coeluting impurities (especially in the front and tail of the main component differing by orders of magnitude) is an easier alternative to finding complementary column/s, as hydrophobicity dominates reversed-phase separation. The same phase separation is practical as relative levels of impurities and main component in some transferred fractions are comparable enabling their separation. The results were confirmed using mass spectrometry. This work has significant bearing as a method assessment tool in pharmaceutical and other industries.
Technology evaluation: huN901-DM1, ImmunoGen. (2005)

ImmunoGen is developing huN901-DMI, a compound comprised of a CD56-targeted humanized N901 antibody conjugated to the company's proprietary cytotoxic agent. DM1, using its tumor-activated prodrug technology, for the potential treatment of cancers that express CD56, in particular, small-cell lung cancer.
The divergent DSL ligand Dll3 does not activate Notch signaling but cell autonomously attenuates signaling induced by other DSL ligands (2005)

Mutations in the DSL (Delta, Serrate, Lag2) Notch (N) ligand Delta-like (Dll) 3 cause skeletal abnormalities in spondylocostal dysostosis, which is consistent with a critical role for N signaling during somitogenesis. Understanding how Dll3 functions is complicated by reports that DSL ligands both activate and inhibit N signaling. In contrast to other DSL ligands, we show that Dll3 does not activate N signaling in multiple assays. Consistent with these findings, Dll3 does not bind to cells expressing any of the four N receptors, and N1 does not bind Dll3-expressing cells. However, in a cell-autonomous manner, Dll3 suppressed N signaling, as was found for other DSL ligands. Therefore, Dll3 functions not as an activator as previously reported but rather as a dedicated inhibitor of N signaling. As an N antagonist, Dll3 promoted Xenopus laevis neurogenesis and inhibited glial differentiation of mouse neural progenitors. Finally, together with the modulator lunatic fringe, Dll3 altered N signaling levels that were induced by other DSL ligands.
Beta 2-adrenergic receptor regulation of the cardiac L-type Ca2+ channel coexpressed in a fibroblast cell line. (1995)

To characterize the functional coupling of the beta 2-AR to the cardiac Ca2+ channel in a system with a single receptor subtype, we stably cotransfected a Chinese hamster fibroblast (CHW) cell line, which lacks beta 2-ARs and Ca2+ channels, with the rabbit cardiac Ca2+ channel alpha 1 and beta 2 subunits and the human beta 2-AR cDNAs. The effects of beta 2-AR stimulation on the expressed Ca2+ channel current were examined using the whole-cell patch-clamp technique. CHW cells transfected with the Ca2+ channel subunits displayed a voltage-dependent inward current having properties typical of native cardiac L-type Ca2+ channels. The expressed current was increased by a phosphorylation-dependent mechanism. CHW cells cotransfected with the Ca2+ channel subunits and the beta 2-AR were responsive to isoproterenol (Iso) in a dose-dependent manner. Iso (10 microM) increased peak Ca2+ channel current to 172 +/- 5% (n = 17) of control amplitude, indicating that the expressed Ca2+ channels are functionally coupled to the beta 2-AR. The results demonstrate unequivocally that beta 2-ARs can modulate the activity of cardiac Ca2+ channels, independent of beta 1-ARs. The results also demonstrate the usefulness of the CHW heterologous expression system, the first to reconstitute physiological modulation of an L-type Ca2+ channel by the beta 2-AR, for studying receptor subtype-specific regulation of the Ca2+ channel.
Managing security of virtual machine images in a cloud environment (2009)

Cloud computing is revolutionizing how information technology resources and services are used and managed but the revolution comes with new security problems. Among these is the problem of securely managing the virtual-machine images that encapsulate each application of the cloud. These images must have high integrity because the initial state of every virtual machine in the cloud is determined by some image. However, as some of the enefits of the cloud depend on users employing images built by third parties, users must also be able to share images safely.
Similar Bronchodilation with Formoterol Delivered by Aerolizer or Turbuhaler (1999)

Formoterol is similarly effective when used as a dry powder when given by either Aerolizer or the Turbuhaler.
Synthesis of Linear Polyethylenimine Derivatives for DNA Transfection (2003)

A series of linear polymers containing varying amounts of ethylenimine or N-propylethylenimine units were synthesized by hydrolysis and/or reduction of polyethyloxazolines. The pK(a)s of the polyamines were determined potentiometrically. Gel mobility shift assay showed that the efficiency of DNA complexation was related to the fraction of amino groups that are protonated at neutral pH. The effects of cationic charge density and molar weight of the polymers on the transfection efficiency were evaluated on HepG2 cells. The results obtained with different copolymers show that the transfection efficiency primarily depends on the fraction of ethylenimine units included in the polymer albeit the molar weight is also of importance. On the basis of the results obtained with poly(N-propylethylenimines), we also demonstrate that the high transfection efficiency of polyethylenimines does not solely rely on their capacity to capture protons which are transferred into the endo-lysosomes during acidification.
Model-based resource selection for efficient virtual cluster deployment (2007)

Virtual clusters on Grids can greatly extend the current scale and efficiency of high-performance Grid computing through more flexible usage of distributed heterogeneous resources. However, the overhead due to installing virtual clusters often precludes their applicability only to long-running applications. This is especially problematic on heterogeneous Grid environments, since installation time of each virtual node can vary greatly, and the total installation time of a virtual cluster is bottlenecked by the slowest node. To achieve fast virtual cluster installation even on such heterogeneous Grid environments, we propose a model-based resource selection policy that automatically identifies a near-optimal node combination to assemble each virtual cluster. We divide the VM setup process into five logical steps and construct a performance model for each step. The model represents the execution time of each step as a linear combination of hardware and software parameters, including CPU frequency, disk I/O performance, and installed package size. To evaluate the proposed resource selection policy, we have extended our own virtual cluster installer, VPC, to select nodes in the increasing order of predicted installation time. Experimental results show that the model-based selection policy is indeed effective, especially when the package size differs depending on sites. The proposed policy has shown to reduce the installation time by up to 68% compared to the most naïve policy that selects nodes in a random order, 60% and 58% to the policies considering either CPU speed or disk I/O performance, respectively.
Anorectal sepsis and fistula-in-ano. (2008)

Anorectal abscesses and fistulas are common maladies that are usually readily diagnosed and effectively treated. However, complex fistulous disease challenges even the most expert of surgical specialists. The management options in this subset of patients are suboptimal, with treatment often requiring multiple procedures, putting the patient at risk for continued symptoms and fecal incontinence. In this chapter, the clinical aspects of perianal suppurative disease are discussed and an attempt is made to deal with many of the more challenging aspects of patient management.
Face recognition using Laplacianfaces (2005)

We propose an appearance-based face recognition method called the Laplacianface approach. By using Locality Preserving Projections (LPP), the face images are mapped into a face subspace for analysis. Different from Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) which effectively see only the Euclidean structure of face space, LPP finds an embedding that preserves local information, and obtains a face subspace that best detects the essential face manifold structure. The Laplacianfaces are the optimal linear approximations to the eigenfunctions of the Laplace Beltrami operator on the face manifold. In this way, the unwanted variations resulting from changes in lighting, facial expression, and pose may be eliminated or reduced. Theoretical analysis shows that PCA, LDA, and LPP can be obtained from different graph models. We compare the proposed Laplacianface approach with Eigenface and Fisherface methods on three different face data sets. Experimental results suggest that the proposed Laplacianface approach provides a better representation and achieves lower error rates in face recognition.
Src kinase associates with a member of a distinct subfamily of protein-tyrosine phosphatases containing an ezrin-like domain. (1994)

A 6.2-kb full-length clone encoding a distinct protein-tyrosine phosphatase (PTP; EC 3.1.3.48), PTPD1, was isolated from a human skeletal muscle cDNA library. The cDNA encodes a protein of 1174 amino acids with N-terminal sequence homology to the ezrin-band 4.1-merlin-radixin protein family, which also includes the two PTPs H1 and MEG1. The PTP domain is positioned in the extreme C-terminal part of PTPD1, and there is an intervening sequence of about 580 residues without any apparent homology to known proteins separating the ezrin-like and the PTP domains. Thus, PTPD1 and the closely related, partially characterized, PTPD2 belong to the same family as PTPH1 and PTPMEG1, but because of distinct features constitute a different PTP subfamily. Northern blot analyses indicate that PTPD1 and PTPD2 are expressed in a variety of tissues. In transient coexpression experiments PTPD1 was found to be efficiently phosphorylated by and associated with the src kinase pp60src.
Optical router for optical fiber sensor networks based on a liquid crystal cell (2003)

Optical fiber sensor networks are evolving rapidly. They are used because of the inert nature of optical fibers allowing no electromagnetic interference and safe applications in inflammable atmospheres; other relevant characteristics are their low weights and wide bandwidths as a transmission medium. In any case, it is very interesting to have specific components such as optical routers for selecting a certain path in a network with no optical to electrical and electrical to optical conversions. In this paper, we propose an all-optical router based on liquid crystals, polarizers, and a spatial split polarization beam splitter. The implemented device is designed to operate with visible light and it has been tested with plastic optical fibers. It has a crosstalk of 14 dB between selected ON channels and nonoperative OFF channels and 11-dB insertion losses. An average switch time of 100 ms is measured. The device checks the optical power level in each channel and, in case of failure, automatically switches to an operative channel while an alarm is activated.