gebhart/patrepeval huggingface dataset:
The Role of Analytical Models in Strategic Management (1993)

The Annual Meeting of the American Association for Cancer Research (AACR) is the world's largest and most comprehensive gathering of cancer researchers. At the 2008 AACR Annual Meeting, innovative research approaches, novel technologies, potentially life-saving therapies in the pipeline, late-breaking clinical trial findings, and new approaches to cancer prevention were presented by top scientists. Reflecting the global state of cancer research with an eye toward future trends, several areas of great science and discovery in the cancer field were shared in this report, which include cancer biomarkers, the role of microRNAs in cancer research, cancer stem cells, tumor microenvironment, targeted therapy, and cancer prevention. This article presents an overview of hot topics discussed at the 2008 AACR Annual Meeting and recapitulates some scientific sessions geared toward new technologies, recent progress, and current challenges reported by cancer researchers. For those who did not attend the meeting, this report may serve as a highlight of this important international cancer research meeting.
Changes in cytoskeletal proteins in the rat facial nucleus following axotomy (1988)

Changes in L-35S-methionine incorporation into cytoskeletal proteins of the facial nucleus of the rat were studied at various times after unilateral crush or resection of the facial nerve by using 2-dimensional gel electrophoresis and fluorography. We found an increase in labeling of actin and tubulin and a decrease in the 68 kDa and 150 kDa neurofilament polypeptides (200 kDa was not studied). The increase in actin and decrease in neurofilament polypeptide labeling was already significant by 24 hr after nerve resection. These changes were more pronounced after nerve resection than after nerve crush on day 7; actin labeling increased to 270%, and tubulin to 205% of contralateral normal nuclei after resection, whereas both proteins increased to only 165% after crush. Neurofilament labeling decreased to 28% of the contralateral side after resection and to 50% after crush. Immunocytochemistry with a monoclonal antibody to the 150 kDa neurofilament component revealed decreased immunoreactivity in the axotomized facial axons at the inner facial genu, 1 cm proximal to the crush. In contrast, neurofilament immunoreactivity was not decreased in the axotomized perikarya of the facial motoneurons. All changes returned to normal 3 weeks after crush. When axonal regeneration was impeded by nerve resection, incorporation into tubulin remained elevated and into neurofilament proteins remained depressed. Actin returned to normal after either nerve resection or crush. We conclude that the synthesis of tubulin and neurofilament proteins following axotomy is regulated by successful axonal regeneration and/or target contact. Actin synthesis seems to be regulated independently of target-derived factors.
Utility of Oligonucleotide Array–Based Comparative Genomic Hybridization for Detection of Target Gene Deletions (2008)

these examples illustrate the successful use of custom oligonucleotide arrays to detect either whole-gene deletions or intragenic exonic deletions. This technology may be particularly useful as a complementary diagnostic test in the context of a recessive disease when only one mutant allele is found by sequencing.
Current development in non-invasive glucose monitoring (2007)

Painless control of blood glycemic levels could improve life quality of diabetes patients, enabling a better regulation of hyper- and hypoglycaemia episodes and thereby avoiding physiological complications. Although research groups have been trying for decades to separate non-invasive glucose information from interference compounds, none of the available commercial devices offers enough precision to replace lancet approaches. Many reviews have already been published on this topic, but the great amount of information available and the fast development of technologies require a continuous update in the research status. Besides the description of current in-vivo methods and the analysis of devices available commercially, one also explains treatment algorithms useful for multivariate analysis.
Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection (2015)

Acquiring the earliest possible identification of pathogenic microorganisms is critical for selecting the appropriate antimicrobial therapy in infected patients. We herein report the novel "melting temperature (Tm) mapping method" for rapidly identifying the dominant bacteria in a clinical sample from sterile sites. Employing only seven primer sets, more than 100 bacterial species can be identified. In particular, using the Difference Value, it is possible to identify samples suitable for Tm mapping identification. Moreover, this method can be used to rapidly diagnose the absence of bacteria in clinical samples. We tested the Tm mapping method using 200 whole blood samples obtained from patients with suspected sepsis, 85% (171/200) of which matched the culture results based on the detection level. A total of 130 samples were negative according to the Tm mapping method, 98% (128/130) of which were also negative based on the culture method. Meanwhile, 70 samples were positive according to the Tm mapping method, and of the 59 suitable for identification, 100% (59/59) exhibited a "match" or "broad match" with the culture or sequencing results. These findings were obtained within three hours of whole blood collection. The Tm mapping method is therefore useful for identifying infectious diseases requiring prompt treatment.
Preferential glial cell attachment to microcontact printed surfaces (1997)

Microcontact printing is introduced as a method for fabricating test surfaces for attachment of cells to chemically patterned silicon surfaces. Tests with astroglial cells indicate that cells attach to microcontact printed surfaces similarly to surfaces produced by traditional photolithographic methods. Astroglial cells attach selectively to 50 microns wide bars of N1[3-(Trimethoxysilyl)propyl]diethylenetriamine (DETA) self-assembled monolayers (SAMs) on surfaces prepared using variable width spaces generated from microcontact printing with octadecyltrichlorosilane (OTS) as the ink. Our results demonstrate that microcontact printing provides an effective and rapid method for routine production of patterned self-assembled monolayers that can be used for directing cell attachment and studying cell morphology.
Development and Validation of a Novel Plasma Protein Signature for Breast Cancer Diagnosis by Using Multiple Reaction Monitoring-based Mass Spectrometry. (2015)

We developed a distinct plasma protein signature for breast cancer diagnosis based on an MRM-based approach, and the clinical value of the 3-protein signature was validated in an independent cohort.
Nonlinear microwave circuits (1988)

This classic text is an excellent resource and time-saver for engineers who need tackle troublesome nonlinear components that remain in use despite recent advances in microwave technology. NONLINEAR MICROWAVE CIRCUITS offers detailed, technically substantial coverage of key methods for the analysis, design, and optimization of nonlinear microwave circuits. Using minimal mathematics, it integrates in-depth, readable coverage of the underlying theories that guide these methods. This book is replete with valuable how to information on a wide range of topics.
Heritability of death from coronary heart disease: a 36‐year follow‐up of 20 966 Swedish twins (2002)

The genetic contribution to the variation in CHD-mortality was moderate both in females and males. Furthermore, although genetic effects appeared to be greater at younger ages of death, our findings clearly suggest that genetic factors are in operation throughout the entire life span.
High Mobility Group Box 1 Protein Binding to Lipopolysaccharide Facilitates Transfer of Lipopolysaccharide to CD14 and Enhances Lipopolysaccharide-Mediated TNF-α Production in Human Monocytes (2008)

LPS-binding protein (LBP) is a central mediator that transfers LPS to CD14 to initiate TLR4-mediated proinflammatory response. However, a possibility of another LPS transfer molecule has been suggested because LBP-deficient mice showed almost normal inflammatory response after LPS injection. In this study, we describe the novel finding that high mobility group box 1 protein (HMGB1) recently identified as a mediator of sepsis has a function of LPS transfer for a proinflammatory response. We used ELISA and surface plasmon resonance to show that HMGB1 binds LPS in a concentration-dependent manner and that the binding is stronger to lipid A moiety than to the polysaccharide moiety of LPS. This binding was inhibited by LBP and polymyxin B. Using native PAGE and fluorescence-based LPS transfer analyses, we show that HMGB1 can catalytically disaggregate and transfer LPS to both soluble CD14 protein and to human PBMCs in a dose-dependent manner. However, this effect was dramatically reduced to the baseline level when HMGB1 was heat inactivated. Furthermore, a mixture of HMGB1 and LPS treatment results in a higher increase in TNF-alpha production in human PBMCs and peripheral blood monocytes than LPS or HMGB1 treatment alone or their summation. Thus, we propose that HMGB1 plays an important role in Gram-negative sepsis by catalyzing movement of LPS monomers from LPS aggregates to CD14 to initiate a TLR4-mediated proinflammatory response.
The trustworthy digital camera: restoring credibility to the photographic image (1993)

The trustworthy digital camera is an application of existing technology toward the solution of an evermore-troubling social problem, the eroding credibility of the photographic image. Although it will always be possible to lie with a photograph (using such time-honored techniques as false perspective and misleading captions), this proposed device will prevent the explosion of very capable personal computers from driving up the incidence of doctored photographs being passed off as truth. A solution to this problem comes from the proposed digital signature standard (DSS), which incorporates modern cryptographic techniques to authenticate electronic mail messages.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Synthesis and Inhibition Properties of Conformational Probes for the Mutase‐Catalyzed UDP‐Galactopyranose/Furanose Interconversion (2003)

UDP-galactose mutase is a flavoenzyme that catalyzes the isomerization of UDP-galactopyranose into UDP-galactofuranose, a key step in the biosynthesis of important bacterial oligosaccharides. Several mechanisms for this unique ring-contraction have been proposed, one of them involving a putative 1,4-anhydrogalactopyranose as an intermediate in the reaction. The purpose of this study was to probe the mutase binding site with conformationally restricted analogues of its substrate. Thus, we describe the straightforward synthesis of two C-glycosidic UDP-galactose derivatives: analogue 1, presenting a galactose moiety locked in a bicyclic (1,4)B boat conformation, and UDP-C-Galf 2, where the galactose residue is locked in the conformation of the mutase substrate. The two molecules were found to be inhibitors of UDP-galactose mutase at levels depending on the redox state of the enzyme. Strong inhibition of the native enzyme, but a low one of the reduced mutase, were observed with UDP-C-Galf 2, whereas 1 displayed intermediate inhibition levels under both native and reducing conditions. These data provide evidence of a significant conformational difference of the mutase binding pocket in the reduced enzyme and in the native one, the enzyme switching from a low Galf-affinity state (reduced enzyme) to a very strong one (native enzyme). It is remarkable that the mutase binds the boat-locked analogue 1 with similar affinities in both its conformational states. These results support a mechanism involving the formation of 1,4-anhydrogalactopyranose as a low-energy intermediate. An alternative explanation would be that the distortion of the galactose moiety during the cycle contraction transiently brings the carbohydrate into a conformation close to a (1,4)B boat.
Magnetic Composite Nanoparticle of Au/γ-Fe2O3 Synthesized by Gamma-Ray Irradiation (2003)

Abstract Magnetic composite nanoparticle of Au/γ-Fe2O3 was synthesized in an aqueous phase using gamma-ray. Connection between gold and γ-Fe2O3 was confirmed by the magnetic separation technique. TEM observation shows that 5-nm gold particles were dispersed on 20-nm γ-Fe2O3 particles. The nanoparticles adsorbed a water-soluble mercaptan, glutathione, and was manipulated by an external magnetic field.
The dynamically reconfigurable CAP Array Chip I (1986)

Array Chip I is a bit-slice processor chip using novel fault-tolerant, large-area integration techniques. It provides 20 1-bit bit-slice processors, or cells, which are controlled from a single microinstruction stream. The chip is implemented in 3-/spl mu/m double-metal n-well CMOS, has 115000 transistors, and an unusually large die size of 500/spl times/650 mil. Prototype yields, with at least 16 cells operational exceed 25%. The chip configuration is controlled by software at run time to form bit-parallel or bit-serial words of arbitrary size and to exclude defective elements. Following system initialization, defective elements are invisible at both the programming and the physical chip interconnect levels. Thirty-six of these chips have been used in a prototype cellular-array parallel processor employing the single-instruction stream, multiple-data stream (SIMD) architecture.
Anti-PEG antibodies: Properties, formation, testing and role in adverse immune reactions to PEGylated nano-biopharmaceuticals (2020)

Conjugation of polyethylene glycols (PEGs) to proteins or drug delivery nanosystems is a widely accepted method to increase the therapeutic index of complex nano-biopharmaceuticals. Nevertheless, these drugs and agents are often immunogenic, triggering the rise of anti-drug antibodies (ADAs). Among these ADAs, anti-PEG IgG and IgM were shown to account for efficacy loss due to accelerated blood clearance of the drug (ABC phenomenon) and hypersensitivity reactions (HSRs) entailing severe allergic symptoms with occasionally fatal anaphylaxis. In addition to recapitulating the basic information on PEG and its applications, this review expands on the physicochemical factors influencing its immunogenicity, the prevalence, features, mechanism of formation and detection of anti-PEG IgG and IgM and the mechanisms by which these antibodies (Abs) induce ABC and HSRs. In particular, we highlight the in vitro, animal and human data attesting to anti-PEG Ab-induced complement (C) activation as common underlying cause of both adverse effects. A main message is that correct measurement of anti-PEG Abs and individual proneness for C activation might predict the rise of adverse immune reactions to PEGylated drugs and thereby increase their efficacy and safety.
Differential regulation of biglycan and decorin by retinoic acid in bovine chondrocytes. (1992)

The small, leucine-rich proteoglycans, decorin and biglycan, are prominent components of many extracellular matrices and are differentially regulated in various tissues.We have examined the effects of retinoic acid (RA) on the expression of biglycan and decorin at the protein and mRNA levels in cultured bovine articular chondrocytes.Biglycan protein expression is rapidly turned off after 1-2 days of treatment with RA.In contrast, decorin protein expression is increased 12-18-fold following 3 days of RA treatment.The level of biglycan mRNA was also rapidly reduced upon RA treatment, mirroring the protein expression.The reduction was apparent by 6 h, and, by 4 days, the levels were nearly undetectable.In contrast, decorin mRNA was induced upon treatment with RA.The increase in decorin message levels was first apparent by 2 4 h, reaching maximum by 2 days, and remained constant through 4 days.The repression of biglycan mRNA displayed equal sensitivity to RA concentrations from lo-' to lo-@ M. Decorin mRNA was induced in a dose-dependent fashion by RA.Retinoic acid at a concentration of lo-' M, the highest dose examined, resulted in maximal induction of the message, and control levels were obtained with lo-* M.The protein synthesis inhibitor cycloheximide inhibited the induction of decorin mRNA, indicating that the induction by RA was a secondary event.In contrast, the repression of biglycan by RA was not significantly altered by cycloheximide, showing that the repression was a direct effect.Actinomycin D inhibited the induction of decorin mRNA, indicating that transcription was required for the induction.Nuclear run-on assays confirmed that RA was regulating biglycan mRNA expression at the transcription level.A 24-h RA treatment decreased the level of transcription of the biglycan gene 6-fold.In contrast, no increase in transcription from the decorin gene could be detected by nuclear run-on assays.Therefore, the elevation in decorin mRNA levels observed after RA treatment was the result of a post-transcriptional event, most likely the consequence of stabilization of the message.This study demonstrates that the genes for these two similar proteoglycans are under very different forms of regulation by RA in chondrocytes.The pattern of differential expression of biglycan and decorin could
Performance of Random Linear Network codes concatenated with Reed-Solomon codes using turbo decoding (2010)

In this paper, we consider a broadcast application for mobile terminals where the access points are connected to the source via a fixed network. Random Linear Network (RLN) coding is used in the fixed network to maximize its transmission rate and a wireless connection is used between the access points and the terminal. In order to reduce variations in the transmission delay and offer more flexibility to the terminal, we perform the network decoding in the terminal. We investigate the error correction capability of RLN coding using the ordered statistics decoding (OSD) algorithm. Taking into account the forward error correcting (FEC) code used on the wireless link, a product code is built by combining the FEC code and the RLN code and turbo decoded in the terminal. The proposed scheme efficiency is analyzed through its simulated and theoretical performance under additive white Gaussian noise (AWGN) and non frequency-selective block Rayleigh fading channels.
Targeted modification and transportation of cellular proteins (2000)

Peptide aptamers are proteins selected from combinatorial libraries that display conformationally constrained variable regions. Peptide aptamers can disrupt specific protein interactions and thus represent a useful method for manipulating protein function in vivo. Here, we describe aptamer derivatives that extend the range of functional manipulations. We isolated an aptamer with increased affinity for its Cdk2 target by mutagenizing an existing aptamer and identifying tighter binding mutants with calibrated two-hybrid reporter genes. We used this and other anti-Cdk2 aptamers as recognition domains in chimeric proteins that contained other functional moieties. Aptamers fused to the catalytic domain of a ubiquitin ligase specifically decorated LexA-Cdk2 with ubiquitin moieties in vivo. Aptamers against Cdk2 and another protein, Ste5, that carried a nuclear localization sequence transported their targets into the nucleus. These experiments indicate that fusion proteins containing aptameric recognition moieties will be useful for specific modification of protein function in vivo.
Structural heterogeneity and protein composition of exosome‐like vesicles (prostasomes) in human semen (2008)

From the data reported herein, we hypothesize that the structural heterogeneity of the exosome-like particles in human semen reflects their functional diversity. Our detailed proteomic analysis provided a list of candidate proteins for future structural and functional studies.
Complete amino acid sequence of variable domains from two monoclonal human anti-gamma globulins of the Wa cross-idiotypic group: suggestion that the J segments are involved in the structural correlate of the idiotype. (1981)

The complete amino acid sequences of the variable regions of two human IgM anti-gamma globulins are reported. The two proteins, Sie and Wol, share idiotypic determinants that are distinct from those shared by the two previously sequenced IgM anti-gamma globulins, Lay and Pom [Capra, J. D. & Kehoe, J. M. (1974) Proc. Natl. Acad. Sci. USA 71, 4032-4036; Klapper, D. G. & Capra, J. D. (1976) Ann. Immunol. (Inst. Pasteur) 127C, 261-271.] In contrast to Lay and Pom, Sie and Wol have very little homology in the complementarity-determining hypervariable regions of either their light or heavy polypeptide chains. However, there are only two amino acid differences in the framework portions of the light chains, and the J segments of both chains are homologous. These data suggest that the predominant idiotypic determinant in this cross-idiotypic system may be different than in the Po group and may be either related to their light chain framework structures or to the amino acid sequence in the J segments of these molecules.
Anti-tumor necrosis factor alpha therapy suppresses the induction of experimental autoimmune uveoretinitis in mice by inhibiting antigen priming. (1996)

Neutralization of systemic TNF ameliorates EAU. The effectiveness of afferent treatment in comparison to the treatment during the efferent stage, together with the reduced proliferation and the reduced abundance of IRBP-responsive cells, suggest that interference with afferent-acting processes such as antigen priming is important to achieve protection from EAU by anti-TNF treatment.
Retargeting NK‐92 for anti‐melanoma activity by a TCR‐like single‐domain antibody (2013)

The efficacy of immunotherapy based on natural killer (NK) cells is hampered by intrinsic non-specific cytotoxicity and insufficient activation of NK cells. Here, we confer the T-cell receptor-like (TCR-like) specificity on NK cells, taking advantage of both the innate and adaptive immune arms of the immune response to generate enhanced anti-melanoma activity. The TCR-like antibody (Ab) GPA7 was selected against melanoma-associated gp100/human leukocyte antigen (HLA)-A2 complex and then fused to intracellular domain of CD3-ζ chain. This fusion construct was incorporated into NK-92MI cell line and expressed as a chimeric antigen receptor on the surface of the cell. The anti-tumour activity of the transgenic NK-92MI-GPA7-ζ cell line was assessed against melanoma in vitro and in vivo. The engineered NK-92MI-GPA7-ζ cells could recognize melanoma cells in the context of HLA-A2 and showed enhanced killing of both melanoma cell lines and primary melanoma. Furthermore, adoptively transferred NK-92MI-GPA7-ζ cells significantly suppressed the growth of human melanoma in a xenograft model in mice. Collectively, these results demonstrate that the TCR-like Ab, GPA7, could redirect NK cells to target the intracellular antigen gp100 and enhance anti-melanoma activity, providing a promising immunotherapeutic strategy to prevent and treat melanoma.