gebhart/patrepeval huggingface dataset:
Micronutrients in pregnancy (2001)

Vitamins and minerals, referred to collectively as micronutrients, have important influences on the health of pregnant women and the growing fetus. Iron deficiency results in anemia which may increase the risk of death from hemorrhage during delivery, but its effects on fetal development and birth outcomes is still unclear. Folic acid deficiency can lead to hematological consequences, pregnancy complications and congenital malformations, but again the association with other birth outcomes is equivocal. Zinc deficiency has been associated in some, but not all studies with complications of pregnancy and delivery, as well as with growth retardation, congenital abnormalities and retarded neurobehavioral and immunological development in the fetus. Iodine deficiency during pregnancy results in cretinism and possible fetal wastage and preterm delivery. Deficiency of other minerals such as magnesium, selenium, copper, and calcium have also been associated with complications of pregnancy, childbirth or fetal development. Deficiencies of vitamins other than folate may likewise be related to such complications; and vitamin A or beta-carotene supplements in pregnancy reduced maternal mortality by 50 % in a controlled trial in Nepal. Additional research is need on the prevalence of such deficiencies and their consequences and on cost-effective public health interventions for their control.
Global Topology Analysis of the Escherichia coli Inner Membrane Proteome (2005)

The protein complement of cellular membranes is notoriously resistant to standard proteomic analysis and structural studies. As a result, membrane proteomes remain ill-defined. Here, we report a global topology analysis of the Escherichia coli inner membrane proteome. Using C-terminal tagging with the alkaline phosphatase and green fluorescent protein, we established the periplasmic or cytoplasmic locations of the C termini for 601 inner membrane proteins. By constraining a topology prediction algorithm with this data, we derived high-quality topology models for the 601 proteins, providing a firm foundation for future functional studies of this and other membrane proteomes. We also estimated the overexpression potential for 397 green fluorescent protein fusions; the results suggest that a large fraction of all inner membrane proteins can be produced in sufficient quantities for biochemical and structural work.
Enhanced Secretion of Heterologous Proteins in Pichia pastoris Following Overexpression of Saccharomyces cerevisiae Chaperone Proteins (2006)

In Pichia pastoris, secretory proteins are folded and assembled in the endoplasmic reticulum (ER). However, upon introduction of foreign proteins, heterologous proteins are often retained in the cytoplasm or in the ER as a result of suboptimal folding conditions, leading to protein aggregation. The Hsp70 and Hsp40 chaperone families in the cytoplasm or in ER importantly regulate the folding and secretion of heterologous proteins. However, it is not clear which single chaperone is most important or which combination optimally cooperates in this process. In the present study we evaluated the role of the chaperones Kar2p, Sec63, YDJ1p, Ssa1p, and PDI from Saccharomyces cerevisiae. We found that the introduction of Kar2p, Ssa1p, or PDI improves protein secretion 4-7 times. In addition, we found that the combination chaperones of YDJ1p/PDI, YDJ1p/Sec63, and Kar2p/PDI synergistically increase secretion levels 8.7, 7.6, and 6.5 times, respectively. Therefore, additional integration of chaperone genes can improve the secretory expression of the heterologous protein. Western blot experiments revealed that the chaperones partly relieved the secretion bottleneck resulting from foreign protein introduction in P. pastoris. Therefore, the findings from the present study demonstrate the presence of a network of chaperones in vivo, which may act synergistically to increase recombinant protein yields.
Knockdown of NAT12/NAA30 reduces tumorigenic features of glioblastoma-initiating cells (2015)

NAT12/NAA30 plays an important role in growth and survival of GICs possibly by regulating hypoxia response (HIF1α), levels of p-MTOR (Ser2448) and the p53 pathway.
Prediction of gasoline octane numbers from near-infrared spectral features in the range 660-1215 nm (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPrediction of gasoline octane numbers from near-infrared spectral features in the range 660-1215 nmJeffrey J. Kelly, Clyde H. Barlow, Thomas M. Jinguji, and James B. CallisCite this: Anal. Chem. 1989, 61, 4, 313–320Publication Date (Print):February 15, 1989Publication History Published online1 May 2002Published inissue 15 February 1989https://pubs.acs.org/doi/10.1021/ac00179a007https://doi.org/10.1021/ac00179a007research-articleACS PublicationsRequest reuse permissionsArticle Views1248Altmetric-Citations204LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
E. coli‐expressed recombinant norovirus capsid proteins maintain authentic antigenicity and receptor binding capability (2004)

The baculovirus expression system has been widely used to produce the capsid proteins of Norovirus (NV) and the proteins form virus-like particles (VLPs) that are useful in many studies, such as immunology, diagnosis, and host-receptor interaction. We report here the application of the E. coli expression system in the production of recombinant NV capsid proteins. In a direct comparison of a previous well-characterized NV strain (VA387), we have demonstrated that the E. coli-expressed capsid proteins maintain the same antigenicity and receptor binding specificity as that of the baculovirus-expressed capsid, although the E. coli-expressed VA387 proteins did not form VLPs. Using the E. coli-expression system, we characterized the receptor-binding patterns of three additional NV strains (OIF1998, Parris Island and VA115), in which OIF1998 binds to HBGA of nonsecretors but did not bind or binds weakly to the HBGA of secretors, as seen in strain VA207. Parris Island binds to HBGA of types A and B but not type O secretors and nonsecretors. VA115 did not show specific binding to any A, B, O secretor nor nonsecretor, which is also observed when the capsid protein of this strain was expressed in baculovirus. Our data indicate that VLP formation is not required for receptor binding, and that the bacteria expression system offers a simple alternative for large production of NV capsid protein for various research purposes, particularly for strains generating low yields in the insect cells.
Immunization with a soluble CD4-gp120 complex preferentially induces neutralizing anti-human immunodeficiency virus type 1 antibodies directed to conformation-dependent epitopes of gp120 (1994)

Preservation of the conformation of recombinant gp120 in an adjuvant, enabling it to elicit conformation-dependent, epitope-specific, broadly neutralizing antibodies, may be critical for the development of any gp120-based human immunodeficiency virus type 1 (HIV-1) vaccine. It was hypothesized that recombinant gp120 complexed with recombinant CD4 could stabilize the conformation-dependent neutralizing epitopes and effectively deliver them to the immune system. Therefore, a soluble CD4-gp120 complex in Syntex adjuvant formulation was tested with mice for its ability to induce neutralizing anti-gp120 antibody responses. Seventeen monoclonal antibodies (MAbs) were generated and characterized. Immunochemical studies, neutralization assays, and mapping studies with gp120 mutants indicated that the 17 MAbs fell into three groups. Four of them were directed to what is probably a conformational epitope involving the C1 domain and did not possess virus-neutralizing activities. Another four MAbs bound to V3 peptide 302-321 and exhibited cross-reactive gp120 binding and relatively weak virus-neutralizing activities. These MAbs were very sensitive to amino acid substitutions, not only in the V3 regions but also in the base of the V1/V2 loop, implying a conformational constraint on the epitope. The last group of nine MAbs recognized conformation-dependent epitopes near the CD4 binding site of gp120 and inhibited the gp120-soluble CD4 interaction. Four of these nine MAbs showed broadly neutralizing activities against multiple laboratory-adapted strains of HIV-1, three of them neutralized only HIVIIIB, and the two lower-affinity MAbs did not neutralize any strain tested. Collectively, the results from this study indicate that immunization with the CD4-gp120 complex can elicit antibodies to conformationally sensitive gp120 epitopes, with some of the antibodies having broadly neutralizing activities. We suggest that immunization with CD4-gp120 complexes may be worth evaluating further for the development of an AIDS vaccine.
Functional Analysis of Three Plasmids from Lactobacillus plantarum (2005)

Lactobacillus plantarum WCFS1 harbors three plasmids, pWCFS101, pWCFS102, and pWCFS103, with sizes of 1,917, 2,365, and 36,069 bp, respectively. The two smaller plasmids are of unknown function and contain replication genes that are likely to function via the rolling-circle replication mechanism. The host range of the pWCFS101 replicon includes Lactobacillus species and Lactococcus lactis, while that of the pWCFS102 replicon also includes Carnobacterium maltaromaticum and Bacillus subtilis. The larger plasmid is predicted to replicate via the theta-type mechanism. The host range of its replicon seems restricted to L. plantarum. Cloning vectors were constructed based on the replicons of all three plasmids. Plasmid pWCFS103 was demonstrated to be a conjugative plasmid, as it could be transferred to L. plantarum NC8. It confers arsenate and arsenite resistance, which can be used as selective markers.
Metastatic Colorectal Cancer: From Improved Survival to Potential Cure (2010)

The multidisciplinary management of MCRC incorporating improved systemic and local therapies continues to improve median survival and enlarge the cohort of patients that can be approached with curative intent. Recent technological advances have facilitated a better understanding of tumor biology that promises continued advancements in patient care.
Engineering Myocardial Tissue (2005)

To create an artificial heart is one of the most ambitious dreams of the young field of tissue engineering, a dream that, when publicly announced in 1999 (LIFE initiative around M. Sefton), provoked as much compassion as scepticism in the scientific and lay press. Today, it is fair to state that the field is still far away from having built the “bioartificial heart.” Nevertheless, substantial progress has been made over the past 10 years, and a realistic perspective exists to create 3-dimensional heart muscle equivalents that may not only serve as experimental models but could also be useful for cardiac regeneration.
Fitness and Health (1996)

Part 1 Physical activity and health: health benefits of activity and fitness activity and mental health activity and personal health. Part 2 Aerobic fitness: understanding aerobic fitness aerobic fitness - the training effect improving aerobic fitness aerobic fitness programmes. Part 3 Muscular fitness: understanding muscular fitness benefits of muscular fitness improving your muscular fitness. Part 4 Activity and weight control: nutrition and health energy and activity activity, diet and weight control weight-control programmes. Part 5 Performance in work and sport: performance at work performance in sport the environment and performance. Part 6 Vitality and longevity: age, activity and vitality the psychology of activity activity and the quality of life.
Sympathetic activation cannot fully account for increased plasma renin levels during water deprivation (1997)

This study was designed to determine if the increase in plasma renin activity (PRA) that occurs during water deprivation is mediated by the renal sympathetic nerves or adrenomedullary catecholamine release. Male Sprague-Dawley rats were studied while conscious and unrestrained. In intact or sham-operated rats, 48 h of water deprivation resulted in at least a threefold increase in PRA and plasma renin concentration (PRC) but no significant change in plasma norepinephrine or epinephrine concentration. Renal denervation decreased basal PRA, reduced the magnitude of the dehydration-induced PRA increase by 33%, and abolished the renin-suppressing effect of l-propranolol infusion in water-deprived rats. Adrenal demedullation also reduced basal and water-deprived PRA and PRC. However, even the combination of renal denervation and adrenal demedullation did not prevent a significant renin response to dehydration (control PRA of 1.8 +/- 0.6 ng x ml(-1) x h(-1) to dehydration PRA of 6.8 +/- 1.3 ng x ml(-1) x h(-1), P < 0.05). Therefore, some mechanism in addition to sympathoadrenomedullary activation plays a major role in mediating increased PRA during water deprivation.
An adaptive pulse charging algorithm for lithium batteries (2017)

In this paper, a pulse charge system for lithium based batteries, which adaptively picks the correct charging pulse, is proposed to improve the charging performance in terms of speed and charge efficiency. An experimental setup is designed, implemented and tested on Lithium ion (Li-ion) and Lithium ion Polymer (Li-Po) batteries separately. Both batteries reached up to 3.9 V within 40 minutes and both batteries were able to charged approximately 17 % faster than the conventional Constant Current-Constant Voltage (CC-CV) charger. The proposed system gave 6 % and 5 % of leading charge efficiencies than CC-CV strategy, for Li-ion and Li-Po batteries respectively. Li-Po battery gave slightly leading charging performance by 3 % in terms of charging speed and 2 % in terms of charge efficiency compared to Li-ion battery.
Graphene for VLSI: FET and interconnect applications (2009)

Because of their remarkable physical properties, graphene should be one of the most important Emerging Research Materials (ERM) for not only the front-end but also back-end devices of VLSIs for the next decade. In this paper, we discuss the present status of their material technologies and some issues to be addressed for realizing graphene channels and wiring devices for a future LSI.
An Extremely Stable and Orthogonal DNA Base Pair with a Simplified Three-Carbon Backbone (2004)

A nucleotide C3HQ with a minimal three-carbon backbone displays unprecedented pairing strength and orthogonality in a homopair C3HQ:C3HQ in the presence of one equivalent of Cu2+. The pairing stability in DNA even exceeds the related base pair having the regular 2'-deoxyribose backbone. This discovery of a synergy between an artificial backbone and base-pairing scheme opens new avenues for the economical design of modified oligonucleotides with tailored properties.
Novel proteasome inhibitor PS-341 inhibits activation of nuclear factor-kappa B, cell survival, tumor growth, and angiogenesis in squamous cell carcinoma. (2001)

We have shown that activation of nuclear factor-kappa B (NF-kappa B) promotes cell survival and expression of cytokines such as growth-regulated oncogene-alpha, which can modulate angiogenesis, growth, and metastasis of squamous cell carcinoma (SCC). Activation of NF-kappa B and cytoprotective genes in cancer may result from signal-induced phosphorylation and proteasome-dependent degradation of inhibitor-kappa B. In this study, we examined the effects of the novel proteasome inhibitor PS-341 on activation of NF-kappa B and cell survival, growth, and angiogenesis in murine and human SCC cell lines. PS-341 inhibited activation of NF-kappa B DNA binding and functional reporter activity at concentrations between 10(-8) and 10(-7) M. Cytotoxicity was observed at 10(-7) M in four murine and two human SCC lines, and followed early cleavage of poly(ADP-ribose) polymerase, a marker of caspase-mediated apoptosis. In vivo, PS-341 inhibited growth of murine and human SCC in mice at doses of 1--2 mg/kg given three times weekly, and dose-limiting toxicity was encountered at 2 mg/kg. Tumor growth inhibition was associated with a marked decrease in vessel density. PS-341 inhibited expression of the proangiogenic cytokines growth-regulated oncogene-alpha and vascular endothelial growth factor by SCC in the range at which PS-341 inhibits NF-kappa B. We conclude that PS-341 inhibits activation of NF-kappa B pathway components related to cell survival, tumor growth, and angiogenesis in SCC.
Synthesis and Characterization of Phosphorescent Cyclometalated Platinum Complexes (2002)

The synthesis, electrochemistry, and photophysics of a series of square planar Pt(II) complexes are reported. The complexes have the general structure C(wedge)NPt(O(wedge)O),where C(wedge)N is a monoanionic cyclometalating ligand (e.g., 2-phenylpyridyl, 2-(2'-thienyl)pyridyl, 2-(4,6-difluorophenyl)pyridyl, etc.) and O(wedge)O is a beta-diketonato ligand. Reaction of K(2)PtCl(4) with a HC(wedge)N ligand precursor forms the chloride-bridged dimer, C(wedge)NPt(mu-Cl)(2)PtC(wedge)N, which is cleaved with beta-diketones such as acetyl acetone (acacH) and dipivaloylmethane (dpmH) to give the corresponding monomeric C(wedge)NPt(O(wedge)O) complex. The thpyPt(dpm) (thpy = 2-(2'-thienyl)pyridyl) complex has been characterized using X-ray crystallography. The bond lengths and angles for this complex are similar to those of related cyclometalated Pt complexes. There are two independent molecular dimers in the asymmetric unit, with intermolecular spacings of 3.45 and 3.56 A, consistent with moderate pi-pi interactions and no evident Pt-Pt interactions. Most of the C(wedge)NPt(O(wedge)O) complexes display a single reversible reduction wave between -1.9 and -2.6 V (vs Cp(2)Fe/Cp(2)Fe(+)), assigned to largely C(wedge)N ligand based reduction, and an irreversible oxidation, assigned to predominantly Pt based oxidation. DFT calculations were carried out on both the ground (singlet) and excited (triplet) states of these complexes. The HOMO levels are a mixture of Pt and ligand orbitals, while the LUMO is predominantly C(wedge)N ligand based. The emission characteristics of these complexes are governed by the nature of the organometallic cyclometalating ligand allowing the emission to be tuned throughout the visible spectrum. Twenty-three different C(wedge)N ligands have been examined, which gave emission lambda(max) values ranging from 456 to 600 nm. Well-resolved vibronic fine structure is observed in all of the emission spectra (room temperature and 77 K). Strong spin-orbit coupling of the platinum atom allows for the formally forbidden mixing of the (1)MLCT with the (3)MCLT and (3)pi-pi states. This mixing leads to high emission quantum efficiencies (0.02-0.25) and lifetimes on the order of microseconds for the platinum complexes.
Locus Ceruleus Degeneration Promotes Alzheimer Pathogenesis in Amyloid Precursor Protein 23 Transgenic Mice (2006)

Locus ceruleus (LC) degeneration and loss of cortical noradrenergic innervation occur early in Alzheimer's disease (AD). Although this has been known for several decades, the contribution of LC degeneration to AD pathogenesis remains unclear. We induced LC degeneration with N-(2-chloroethyl)-N-ethyl-bromo-benzylamine (dsp4) in amyloid precursor protein 23 (APP23) transgenic mice with a low amyloid load. Then 6 months later the LC projection areas showed a robust elevation of glial inflammation along with augmented amyloid plaque deposits. Moreover, neurodegeneration and neuronal loss significantly increased. Importantly, the paraventricular thalamus, a nonprojection area, remained unaffected. Radial arm maze and social partner recognition tests revealed increased memory deficits while high-resolution magnetic resonance imaging-guided micro-positron emission tomography demonstrated reduced cerebral glucose metabolism, disturbed neuronal integrity, and attenuated acetylcholinesterase activity. Nontransgenic mice with LC degeneration were devoid of these alterations. Our data demonstrate that the degeneration of LC affects morphology, metabolism, and function of amyloid plaque-containing higher brain regions in APP23 mice. We postulate that LC degeneration substantially contributes to AD development.
In Vitro Percutaneous Absorption: Principles, Fundamentals, and Applications (1991)

Preparation of Human and Animal Skin (R. Bronaugh and S. Collier). Diffusion Cell Design (C. Gummer and H. Maibach). A Flow-Through Diffusion Cell (R. Bronaugh). Individual and Regional Variation with In Vitro Percutaneous Absorption (R. Wester and H. Maibach). Receptor Fluids (S. Collier and R. Bronaugh). Assessment of Vehicle Factors Influencing Percutaneous Absorption (J. Zatz). Cutaneous Metabolism (S. Collier, J. Storm and R. Bronaugh). Effect of Occlusion (D. Bucks, R. Guy and H. Maibach). Skin Storage Conditions (W. Reifenrath and B. Kemppainen). In Vitro Absorption Through Damaged Skin (R. Scott). Localization of Drugs in the Skin (A. Rolland). Expression of Absorption Data (S. Collier and R. Bronaugh). The LPP Theory of Skin Penetration Enhancement (B. Barry). In Vitro Percutaneous Absorption of Alachlor and Atrazine (R. Wester, D. Bucks and H. Maibach). In Vivo-In Vitro Correlations for Hydrophobic Compounds (R. Bronaugh and S. Collier). In Vitro Absorption-Skin Flap Model (J. Riviere). Percutaneous Penetration from Soil: Inhibition or Enhancement? (D. Bucks, R. Wester, and H. Maibach). Short-Term In Vitro Skin Exposure (R. Wester and H. Maibach). Protocol for In Vitro Percutaneous Absorption Studies (R. Bronaugh and S. Collier). Finite Dose In Vitro Percutaneous Permeation Based on the Diffusion Model: Estimation of Pharmacokinetic Parameters and Prediction of the Pharmacokinetics Profiles (K. Kubota and H. Maibach). Effect of Sodium Lauryl Sulfate-Induced Skin Irritation on In Vitro Percutaneous Absorption (Klaus-P. Wilhelm, Christian Surber, and Howard I. Maibach). Percutaneous Absorption in Irritant Dermatitis (K. Wilhelm, C. Surber, and H. Maibach).
Synthesis and Characterization of Carboxylate-Modified Gold Nanoparticle Powders Dispersible in Water (1999)

Carboxylate-modified gold nanoparticles have been synthesized in a single-phase system based on the reduction of hydrogen tetrachloroaurate(III) by sodium borohydride in methanol using mercaptosuccinic acid (MSA) as the stabilizing thiol ligand. Five samples with diameters of 10.2, 10.8, 12.8, 19.4, and 33.6 Å have been prepared as water-redispersible powders through decreasing the initial MSA/HAuCl4 molar ratio from 2.5 to 0.5. These samples were characterized by X-ray diffraction, transmission electron microscopy, elemental analysis, thermogravimetric analysis, ζ-potential measurement, Fourier transform infrared spectroscopy, and UV−vis spectroscopy. The results show that a large number of the particles are fcc single crystals with the polyhedral morphology of a truncated octahedral motif and that a self-assembled monolayer of thiolates has indeed formed through the adsorption of mercapto groups on the gold particle surface; the maximum packing density of the thiolates is 15.23 Å2 per mercapto group. The surface structures of the powders are clearly defined; MSA on the particle surface exists in the form of sodium carboxylate, and one MSA unit combines with one H2O molecule.