gebhart/patrepeval huggingface dataset:
A pulsing electric field (PEF) increases human chondrocyte proliferation through a transduction pathway involving nitric oxide signaling (2008)

A potential treatment modality for joint pain due to cartilage degradation is electromagnetic fields (EMF) that can be delivered, noninvasively, to chondrocytes buried within cartilage. A pulsed EMF in clinical use for recalcitrant bone fracture healing has been modified to be delivered as a pulsed electric field (PEF) through capacitive coupling. It was the objective of this study to determine whether the PEF signal could have a direct effect on chondrocytes in vitro. This study shows that a 30-min PEF treatment can increase DNA content of chondrocyte monolayer by approximately 150% at 72 h poststimulus. Studies intended to explore the biological mechanism showed this PEF signal increased nitric oxide measured in culture medium and cGMP measured in cell extract within the 30-min exposure period. Increasing calcium in the culture media or adding the calcium ionophore A23187, without PEF treatment, also significantly increased short-term nitric oxide production. The inhibitor W7, which blocks calcium/calmodulin, prevented the PEF-stimulated increase in both nitric oxide and cGMP. The inhibitor L-NAME, which blocks nitric oxide synthase, prevented the PEF-stimulated increase in nitric oxide, cGMP, and DNA content. An inhibitor of guanylate cyclase (LY83583) blocked the PEF-stimulated increase in cGMP and DNA content. A nitric oxide donor, when present for only 30 min, increased DNA content 72 h later. Taken together, these results suggest the transduction pathway for PEF-stimulated chondrocyte proliferation involves nitric oxide and the production of nitric oxide may be the result of a cascade that involves calcium, calmodulin, and cGMP production.
Human Relaxins: Chemistry and Biology* (1994)

IT IS more than a decade since the previous and first review in this journal on relaxin (1). There have been many significant advances over this period which have been reviewed by us and others (2–4). The sequencing of the two human relaxin genes termed HI and H2 relaxins, and the availability of these peptides and antibodies have allowed relatively rapid progress in knowledge over the last few years. There is enough accumulated data to warrant specialization within the field and to allow us to focus this review on the human relaxins. Where there are gaps in the knowledge of effects of human relaxin in human systems, they have been filled with data from nonhuman animal experimentation to provide a more complete picture of the status of the field. A summary of the principal advances over the period 1983–1992 is presented in Table 1. Over this period the sequences of the two human relaxin genes as well as their tissue expression were described together with the chemical and recombinant syntheses of their peptides, the development of an homologous RIA, and a method of labeling relaxin with 32P while retaining its biological activity for receptor detection.
Recent Advances of Colony-Stimulating Factor-1 Receptor (CSF-1R) Kinase and Its Inhibitors (2018)

Colony stimulation factor-1 receptor (CSF-1R), which is also known as FMS kinase, plays an important role in initiating inflammatory, cancer, and bone disorders when it is overstimulated by its ligand, CSF-1. Innate immunity, as well as macrophage differentiation and survival, are regulated by the stimulation of the CSF-1R. Another ligand, interlukin-34 (IL-34), was recently reported to activate the CSF-1R receptor in a different manner. The relationship between CSF-1R and microglia has been reviewed. Both CSF-1 antibodies and small molecule CSF-1R kinase inhibitors have now been tested in animal models and in humans. In this Perspective, we discuss the role of CSF-1 and IL-34 in producing cancer, bone disorders, and inflammation. We also review the newly discovered and improved small molecule kinase inhibitors and monoclonal antibodies that have shown potent activity toward CSF-1R, reported from 2012 until 2017.
TraceNET (2010)

This paper presents a network layer Internet topology collection tool called tracenet. Compared to traceroute, tracenet can collect a more complete topology information on an end-to-end path. That is, while traceroute returns a list of IP addresses each representing a router on a path, tracenet attempts to return all the IP addresses assigned to the interfaces on each visited subnetwork on the path. Consequently, the collected information (1) includes more IP addresses belonging to the traced path; (2) represents "being on the same LAN" relationship among the collected IP addresses; and (3) annotates the discovered subnets with their observed subnet masks. Our experiments on Internet2, GEANT, and four major ISP networks demonstrate promising results on the utility of tracenet for future topology measurement studies.
THE HUMAN ERYTHROCYTE Cl‐DEPENDENT Na‐K COTRANSPORT SYSTEM AS A POSSIBLE MODEL FOR STUDYING THE ACTION OF LOOP DIURETICS (1982)

1 The recent demonstration of the chloride-dependence of the red cell Na-K cotransport system suggests an analogy between this process and the active Cl- absorption in the ascending loop of Henle, which is the target transport system for loop diuretics. 2 Using red cell K influx, four known loop diuretics, six experimental frusemide analogues, two thiazides, two K-retaining diuretics and one organomercurial were compared for inhibitory potency on the red cell Na-K cotransport system. 3 Except for mersalyl, whose exact mode of action in the kidney is still in doubt, the inhibition of the red cell system by various loop diuretics was consistent with both published whole body diuretic data and isolated perfused tubule studies, while the system did not respond to the thiazides or the K-retaining diuretics. 4 It is concluded that the human red cell Na-K cotransport system is a possible valid model process on which to study the activity of loop diuretics.
Functional Folate Receptor Alpha Is Elevated in the Blood of Ovarian Cancer Patients (2009)

Our results demonstrate that ovarian cancer patients have elevated levels of functional intact FRalpha. These findings support the potential use of circulating FRalpha as a biomarker of early ovarian cancer.
Mott transition field effect transistor (1998)

A field effect transistor fabricated with an oxide channel has been shown to demonstrate switching characteristics similar to conventional silicon metal oxide field effect transistors. This device is believed to operate via a Mott metal-insulator transition induced by the gate field, and offers a potential technology alternative for the regime beyond silicon scaling limitations.
Notch signaling controls hepatoblast differentiation by altering the expression of liver-enriched transcription factors (2004)

Hepatoblasts give rise to both mature hepatocytes and cholangiocytes. While Notch signaling has been implicated in the formation of bile ducts composed of cholangiocytes, little is known about the mechanism of lineage commitment of hepatoblasts. Here we describe the role of the Notch pathway in hepatoblast differentiation. Immunohistochemical analysis showed that Jagged1 was expressed in the cells surrounding the portal veins and Notch2 was expressed in most hepatic cells at mid gestation when ductal plates are formed surrounding the portal veins. Interestingly, the Jagged1+ cells were adjacent to ductal plates, suggesting that the Notch signaling is activated in hepatoblasts that undergo differentiation into cholangiocytes. In fact, expression of the Notch intracellular domain in Dlk+ hepatoblasts inhibited hepatic differentiation and significantly reduced the expression of albumin, a marker of both hepatoblasts and hepatocytes. Furthermore, the addition of Matrigel to the hepatoblast culture upregulated the expression of cytokeratin 7 and 19, integrin beta4, and HNF1beta, which are known to be expressed in cholangiocytes. By contrast, downregulation of the Notch signaling by siRNA specific for Notch2 mRNA as well as by the gamma-secretase inhibitor L-685,458 promoted the hepatic differentiation. Consistent with the previous finding that mature cholangiocytes strongly express HNF1beta, but barely express HNF1alpha, HNF4, and C/EBPalpha, activation of the Notch signaling upregulated HNF1beta expression, whereas it downregulated the expression of HNF1alpha, HNF4, and C/EBPalpha. These results suggest that the Notch signaling contributes to form a network of these transcription factors suitable for cholangiocyte differentiation.
A Solid Lithium Electrolyte via Addition of Lithium Isopropoxide to a Metal–Organic Framework with Open Metal Sites (2011)

The uptake of LiO(i)Pr in Mg(2)(dobdc) (dobdc(4-) = 1,4-dioxido-2,5-benzenedicarboxylate) followed by soaking in a typical electrolyte solution leads to the new solid lithium electrolyte Mg(2)(dobdc)·0.35LiO(i)Pr·0.25LiBF(4)·EC·DEC (EC = ethylene carbonate; DEC = diethyl carbonate). Two-point ac impedance data show a pressed pellet of this material to have a conductivity of 3.1 × 10(-4) S/cm at 300 K. In addition, the results from variable-temperature measurements reveal an activation energy of just 0.15 eV, while single-particle data suggest that intraparticle transport dominates conduction.
Electrostatic Torsional Micromirror With Enhanced Tilting Angle Using Active Control Methods (2010)

Electrostatic microelectromechanical systems (MEMS)-based torsional micromirrors are a fundamental building block for many optical network applications, such as optical wavelength-selective switches, configurable optical add-drop multiplexers and optical cross-connects. Although the device architecture, materials and fabrication processes determine the micromirrors' functioning space, one major technical challenge to achieving their full performance potentials is the controllability and stability of the tilting angle. In this paper, an electrostatic micromirror is designed and fabricated using a standard MEMS silicon-on-insulator (SOI) process. Active control approaches including gain scheduling and nonlinear proportional and derivative (PD) control are proposed. Both approaches can improve the performance of the mirror tilting and enhance the robustness of the structures to any stochastic perturbations. Furthermore, the nonlinear PD control can eliminate the micromirror “pull-in” phenomenon, hence significantly expanding the mirror tilt range, and as a result achieving enhanced device performance and functionality. The nonlinear PD control method is experimentally implemented and the results demonstrate the effectiveness of the approach.
MHC class I–specific antibody binding to nonhematopoietic cells drives complement activation to induce transfusion-related acute lung injury in mice (2011)

Transfusion-related acute lung injury (TRALI), a form of noncardiogenic pulmonary edema that develops during or within 6 h after a blood transfusion, is the most frequent cause of transfusion-associated death in the United States. Because development of TRALI is associated with donor antibodies (Abs) reactive with recipient major histocompatibility complex (MHC), a mouse model has been studied in which TRALI-like disease is caused by injecting mice with anti-MHC class I monoclonal Ab (mAb). Previous publications with this model have concluded that disease is caused by FcR-dependent activation of neutrophils and platelets, with production of reactive oxygen species that damage pulmonary vascular endothelium. In this study, we confirm the role of reactive oxygen species in the pathogenesis of this mouse model of TRALI and show ultrastructural evidence of pulmonary vascular injury within 5 min of anti-MHC class I mAb injection. However, we demonstrate that disease induction in this model involves macrophages rather than neutrophils or platelets, activation of complement and production of C5a rather than activation of FcγRI, FcγRIII, or FcγRIV, and binding of anti-MHC class I mAb to non-BM-derived cells such as pulmonary vascular endothelium. These observations have important implications for the prevention and treatment of TRALI.
Identification of dividing, determined sensory neuron precursors in the mammalian neural crest (1999)

Sensory and autonomic neurons of the vertebrate peripheral nervous system are derived from the neural crest. Here we use the expression of lineage-specific transcription factors as a means to identify neuronal subtypes that develop in rat neural crest cultures grown in a defined medium. Sensory neurons, identified by expression of the POU-domain transcription factor Brn-3.0, develop from dividing precursors that differentiate within 2 days following emigration from the neural tube. Most of these precursors generate sensory neurons even when challenged with BMP2, a factor that induces autonomic neurogenesis in many other cells in the explants. Moreover, BMP2 fails to prevent expression of the sensory-specific basic helix-loop-helix (bHLH) transcription factors neurogenin1, neurogenin2 and neuroD, although it induces expression of the autonomic-specific bHLH factor MASH1 and the paired homeodomain factor Phox2a in other cells. These data suggest that there are mitotically active precursors in the mammalian neural crest that can generate sensory neurons even in the presence of a strong autonomic-inducing cue. Further characterization of the neurons generated from such precursors indicates that, under these culture conditions, they exhibit a proprioceptive and/or mechanosensory, but not nociceptive, phenotype. Such precursors may therefore correspond to a lineally (Frank, E. and Sanes, J. (1991) Development 111, 895-908) and genetically (Ma, Q., Fode, C., Guillemot, F. and Anderson, D. J. (1999) Genes Dev. 13, in press) distinct subset of early-differentiating precursors of large-diameter sensory neurons identified in vivo.
Nuclear remodelling and early development in cryopreserved, porcine primordial germ cells following nuclear transfer into in vitro-matured oocytes (1995)

Nuclear transfer was conducted in the pig using, as karyoplasts, primordial germ cells which had been cryopreserved. The cytoplasts were presumptive S- or MII-phase, in vitro-matured oocytes, which had been enucleated mechanically. Enucleation was effective in 94.3% of cases. Karyoplasts were introduced into the perivitelline space, in close contact with the cytoplasts, and the complexes fused by electrical stimulation and activation. Activation was successful in 82-88% of nonmanipulated, pulsed oocytes, and in 55% of germ cell-oocyte complexes. The reconstituted embryos were examined for nuclear remodelling and cleavage in vitro. Nuclear swelling was more prominent when MII-phase cytoplasts, rather than S-phase, cytoplasts, were used. After 24 h in culture, the cleavage rate was not significantly different whether blastomeres or primordial germ cells were used as karyoplasts, and whether MII-phase or S-phase cytoplasts were used. However, after 72 h in culture, the developmental rate was higher when MII-phase cytoplasts (75%) were used for the recipients of blastomeres compared with S-phase cytoplasts (38.5%, p < 0.05). Similar tendencies were observed with germ-cell nuclear transfer when inositol was used as medium for electrofusion (60% vs 27.8%, p < 0.05). Furthermore, when MII-phase cytoplasts were used, the nuclear transferred embryos derived from blastomeres developed at a significantly higher rate than from primordial germ cells (37.5%, p < 0.05). We conclude that cryopreserved primordial germ cells are competent to undergo nuclear remodelling and cleavage during 72 h or incubation in vitro to the 4-cell stage, following nuclear transfer to enucleated, activated (S-) or MII-phase oocytes. This experimental system may help to elucidate events in the early development of pig embryo following nuclear transfer using germ cells as karyoplasts.
Studying Digital Imagery of Ancient Paintings by Mixtures of Stochastic Models (2004)

This paper addresses learning-based characterization of fine art painting styles. The research has the potential to provide a powerful tool to art historians for studying connections among artists or periods in the history of art. Depending on specific applications, paintings can be categorized in different ways. In this paper, we focus on comparing the painting styles of artists. To profile the style of an artist, a mixture of stochastic models is estimated using training images. The two-dimensional (2-D) multiresolution hidden Markov model (MHMM) is used in the experiment. These models form an artist's distinct digital signature. For certain types of paintings, only strokes provide reliable information to distinguish artists. Chinese ink paintings are a prime example of the above phenomenon; they do not have colors or even tones. The 2-D MHMM analyzes relatively large regions in an image, which in turn makes it more likely to capture properties of the painting strokes. The mixtures of 2-D MHMMs established for artists can be further used to classify paintings and compare paintings or artists. We implemented and tested the system using high-resolution digital photographs of some of China's most renowned artists. Experiments have demonstrated good potential of our approach in automatic analysis of paintings. Our work can be applied to other domains.
CHAPTER X (2015)

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THE DETECTION OF CAVES BY GRAVITY MEASUREMENTS* (1963)

A bstract Caves will give rise to gravity minima detectable by the gravity meter if of sufficient volume in relation to their depth. The dimensions required for caves of different geometrical forms to give readable gravity effects are shown by curves. Maps are included of small, sharp gravity minima in Iraq which may be caused by solution caverns.
High Molecular Weight Polypropylene through Specifically DesigneZirconocene Catalysts (1992)

A breakthrough for the technical application of zirconium catalysts in isotactic propylene polymerization has been achieved with complexes 1. The highest molecular mass of polypropylene, the best isotacticity, and the highest activity of all catalysts are provided by 1, R1 = R3 = CH3, R2 = i-C3,H7. Hydrogenation of the six-membered ring drastically lowers the activity and the molecular mass obtained.
Quantitative mutant analysis of viral quasispecies by chip-based matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (2001)

RNA viruses exist as quasispecies, heterogeneous and dynamic mixtures of mutants having one or more consensus sequences. An adequate description of the genomic structure of such viral populations must include the consensus sequence(s) plus a quantitative assessment of sequence heterogeneities. For example, in quality control of live attenuated viral vaccines, the presence of even small quantities of mutants or revertants may indicate incomplete or unstable attenuation that may influence vaccine safety. Previously, we demonstrated the monitoring of oral poliovirus vaccine with the use of mutant analysis by PCR and restriction enzyme cleavage (MAPREC). In this report, we investigate genetic variation in live attenuated mumps virus vaccine by using both MAPREC and a platform (DNA MassArray) based on matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Mumps vaccines prepared from the Jeryl Lynn strain typically contain at least two distinct viral substrains, JL1 and JL2, which have been characterized by full length sequencing. We report the development of assays for characterizing sequence variants in these substrains and demonstrate their use in quantitative analysis of substrains and sequence variations in mixed virus cultures and mumps vaccines. The results obtained from both the MAPREC and MALDI-TOF methods showed excellent correlation. This suggests the potential utility of MALDI-TOF for routine quality control of live viral vaccines and for assessment of genetic stability and quantitative monitoring of genetic changes in other RNA viruses of clinical interest.
Dependence of MOSFET hot-carrier aging on PECVD oxide process (1995)

This paper studies the effect of PECVD oxide process in the sandwiched SOG structure for interlevel dielectric applications on MOSFET hot- carrier reliability. Both silane-based oxide (OX) and tetraethylorthosilicate-based oxide (TEOS) were used to form various combinations with SOG as interlevel dielectrics. The influence of N<SUB>2</SUB>O (N<SUB>2</SUB>O-TEOS) and O<SUB>2</SUB> (O<SUB>2</SUB>-TEOS) as the source gas of oxygen during the PECVD TEOS deposition was also studied. Results indicate that O<SUB>2</SUB>-TEOS/SOG/O<SUB>2</SUB>-TEOS increased the number of electron traps in the gate oxide and deteriorate SiO<SUB>2</SUB>/Si interface characteristics, thus reducing device lifetime. In addition, the use of OX to replace any one of the O<SUB>2</SUB>-TEOS layers improves hot-carrier immunity considerably. Finally, N<SUB>2</SUB>-TEOS/SOG/OX is shown to be a very promising process due to its conformal nature over OX/SOG/OX and improved reliability over O<SUB>2</SUB>-TEOS/SOG/OX.
Variable latency caches for nanoscale processor (2007)

Variability is one of the important issues in nanoscale processors. Due to increasing importance of interconnect structures in submicron technologies, the physical location and phenomena such as coupling have an increasing impact on the latency of operations. Therefore, traditional view of rigid access latencies to components wil result in suboptimal architectures. In this paper, we devise a cache architecture with variable access latency. Particularly, we a) develop a non-uniform access level 1 data-cache, b) study the impact of coupling and physical location on level 1 data cache access latencies, and c) develop and study an architecture where the variable latency cache can be accessed while the rest of the pipeline remains synchronous. To find the access latency with different input address transitions and environmental conditions, we first build a SPICE model at a 45nm technology for a cache similar to that of the level 1 data cache of the Intel Prescott architecture. Motivated by the large difference between the worst and best case latencies and the shape of the distribution curve, we change the cache architecture to allow variable latency accesses. Since the latency of the cache is not known at the time of instruction scheduling, we also modify the functional units with the addition of special queues that will temporarily store the dependent instructions and allow the data to be forwarded from the cache to the functional units correctly. Simulations based on SPEC2000 benchmarks show that our variable access latency cache structure can reduce the execution time by as much as 19.4% and 10.7% on average compared to a conventional cache architecture.
RNA-templated DNA ligation for transcript analysis (2001)

Ligase-mediated gene detection has proven valuable for detection and precise distinction of DNA sequence variants. We have recently shown that T4 DNA ligase can also be used to distinguish single nucleotide variants of RNA sequences. Here we describe parameters that influence RNA-templated DNA ligation by T4 DNA ligase. The reaction proceeds much more slowly, requiring more enzyme, compared to ligation of the same oligonucleotides hybridized to the corresponding DNA sequence. The reaction is inhibited at high concentrations of ATP and NaCl and both magnesium and manganese ions can support the reaction. We define reaction conditions where 80% of RNA target molecules can template a diagnostic ligation reaction. Ligase-mediated RNA detection should provide a useful mechanism for sensitive and accurate detection and distinction of RNA sequence variants.
Randomized trial of intravenous iron-induced hypophosphatemia (2018)

ClinicalTrials.gov, NCT02694978FUNDING. AMAG Pharmaceuticals, Inc.Role of the funding source: This study was supported by AMAG Pharmaceuticals, Inc. The academic investigators designed the clinical trial, performed the analyses, and authored the manuscript with input from the coauthors from AMAG Pharmaceuticals, Inc.