gebhart/patrepeval huggingface dataset:
Therapeutic efficacy of a 188Re-labeled alpha-melanocyte-stimulating hormone peptide analog in murine and human melanoma-bearing mouse models. (2005)

The therapy study results revealed that (188)Re-(Arg(11))CCMSH yielded significant therapeutic effects in both B16/F1 murine melanoma- and TXM13 human melanoma-bearing mouse models. (188)Re-(Arg(11))CCMSH appears to be a promising radiolabeled peptide for targeted radionuclide therapy of melanoma.
Novel Protein and Poxvirus-Based Vaccine Combinations for Simultaneous Induction of Humoral and Cell-Mediated Immunity (2005)

The presence of both cell-mediated and humoral immunity is important in protection from and clearance of a number of infectious pathogens. We describe novel vaccine regimens using combinations of plasmid DNA, poxvirus and protein to induce strong Ag-specific T cell and Ab responses simultaneously in a murine model. Intramuscular (i.m.) immunization with plasmid DNA encoding the middle Ag of hepatitis B (DNA) concurrently with a commercial hepatitis B virus (HBV) vaccine (Engerix-B) followed by boosting immunizations with both modified vaccinia virus Ankara (MVA) encoding the middle Ag of HBV and Engerix-B induced high levels of CD4(+) and CD8(+) T cells and high titer Ab responses to hepatitis B surface Ag (HbsAg). Substitution of Engerix-B with adjuvant-free rHBsAg induced similar T cell responses and greatly enhanced Ab levels. Repeated immunizations with recombinant or nonrecombinant MVA mixed with Ag induced higher titers of Abs compared with immunization with either Ag or Engerix-B further demonstrating this novel adjuvant effect of MVA. The poxviruses NYVAC, fowlpox (FP9) and ALVAC, and to a lesser extent, adenovirus, also displayed similar adjuvant properties when used in combination with rHBsAg. The use of poxviruses as an adjuvant for protein to concurrently induce Ag-specific T cells and Abs could be applied to the development of vaccines for many diseases, including HIV and malaria, where both cell mediated and humoral immunity may be important for protection.
Inducible, High-Level Production of Infectious Murine Leukemia Retroviral Vector Particles Pseudotyped with Vesicular Stomatitis Virus G Envelope Protein (1995)

Murine leukemia viruses (MuLV) have been adapted for use as gene transfer vectors for experimental and human gene therapy applications. Their utility for these purposes has been circumscribed by the limited host range and relatively low titer of available producer clones. Pseudotyping of MuLV particles with the vesicular stomatitis virus envelope protein (VSV-G), expressed transiently in cells producing MuLV Gag and Pol proteins, has yielded vector preparations with a broader host range that can be concentrated by ultracentrifugation. We have explored the use of steroid-inducible and tetracycline-modulated promoter systems (necessary because the VSV-G protein is toxic to cells when constitutively expressed) to derive stable producer cell lines capable of substantial production of VSV-G pseudotyped MuLV particles. A packaging cell line and producer clones capable of expressing a chimeric transcription factor, composed of the tetracycline repressor (tetR) and the VP16 trans-activating sequences of herpes simplex virus VP16 gene and containing the VSV-G coding sequences linked to a minimal promoter having seven tandem copies of the tetracycline responsive operator (tetO), exhibited high levels of VSV-G protein expression when cultured in the absence of tetracycline. Vector particles, produced at titers of 10(5)-10(6) infectious colony forming units per ml (cfu/ml), could be concentrated effectively by ultracentrifugation yielding vector preparations having a titer of 10(9) cfu/ml. These cell lines grew normally when VSV-G protein expression was repressed by tetracycline. Such producer clones hold promise for future human gene therapy applications.
ChemInform Abstract: Reactions of Methyl 4‐Aminofurazan‐3‐carboximidate with Nitrogen‐Containing Nucleophiles. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Polymer‐ and Dendrimer‐Bound Ti‐TADDOLates in Catalytic (and Stoichiometric) Enantioselective Reactions: Are pentacoordinate cationic Ti complexes the catalytically active species? (1996)

Abstract α,α,α′,α′‐ T etra a ryl‐1,3‐ d ioxolane‐4,5‐ d imethan ol s (TADDOLs), containing styryl groups either at C(2) of the heterocyclic ring or in the α‐position, were prepared in the usual way ( 18–22, 24, 25 ). These compounds were copolymerized with styrene and divinylbenzene in a suspension, yielding polymers ( 33–40 , Scheme 3 ) as beads with a rather uniform particle‐size distribution (150–45 μm), swellable in common organic solvents. HOCH 2 ‐ and BrCH 2 ‐substituted TADDOLs were also prepared and used for attachement to Merrifield resin or to dendritic molecules ( 23, 26–32 ). The TADDOL moieties in these materials are accessible to form Ti (and Al) complexes ( Scheme 4 ) which can be used as polymer‐ or dendrimer‐bound reagents (stoichiometric) or Lewis acids (catalytic). The reactions studied with these new chiral auxiliaries are: enantioselective nucleophilic additions to aldehydes (of R 2 Zn and RTi(OCHMe 2 ) 3 ; Scheme 5, Table 1 ) and to ketones (of LiAlH 4 , Table 2 ); enantioselective ring opening of meso ‐anhydrides ( Scheme 6); [4+2] and [3+2] cycloadditions of 3‐crotonyl‐1,3‐oxazolidin‐2‐one to cyclopentadiene and to ( Z )‐ N ‐benzylidenephenylamine N ‐oxide ( → 48, 49 , Scheme 7, Tables 3, 4 , and Fig. 5 ). The enantioselectivities reached with most of the polymer‐bound or dendritic TADDOL ligands were comparable or identical to those observed with the soluble analogs. The activity of the polymer‐bound Lewis acids was only slightly reduced as compared with that encountered under homogeneous conditions. Multiple use of the beads (up to 10 times), without decreased performance, has been demonstrated ( Figs. 3 and 4 ). The poorer selectivity in the Diels‐Alder reaction ( Scheme 7a ), induced by the polymer‐bound Cl 2 Ti‐TADDOLate as compared to the soluble one, is taken as an opportunity to discuss the mechanism of this Lewis ‐acid catalysis, and to propose a cationic, trigonal‐bipyramidal complex as the catalytically active species ( Fig. 6 ). It is suggested that similar cations may be involved in other Ti‐TADDOLate‐mediated reactions as well.
A Comprehensive Evaluation of the Activity and Selectivity Profile of Ligands for RGD-binding Integrins (2017)

Integrins, a diverse class of heterodimeric cell surface receptors, are key regulators of cell structure and behaviour, affecting cell morphology, proliferation, survival and differentiation. Consequently, mutations in specific integrins, or their deregulated expression, are associated with a variety of diseases. In the last decades, many integrin-specific ligands have been developed and used for modulation of integrin function in medical as well as biophysical studies. The IC<sub>50</sub>-values reported for these ligands strongly vary and are measured using different cell-based and cell-free systems. A systematic comparison of these values is of high importance for selecting the optimal ligands for given applications. In this study, we evaluate a wide range of ligands for their binding affinity towards the RGD-binding integrins αvβ3, αvβ5, αvβ6, αvβ8, α5β1, αIIbβ3, using homogenous ELISA-like solid phase binding assay.
Microfluidic Chips for Viral RNA Extraction & amp; Detection (2006)

Sensing biomolecules at minute quantities demands laborious and skill-laden laboratory protocols for sample preparation and amplification in order to improve signal-to-noise ratio. Nucleic-acid-based detection of viral particles in whole blood requires separation of viral particles from blood cells followed by extraction, amplification, and detection of viral nucleic acids. Here, three microfluidic chips have been independently shown to be capable of performing each critical step. Separation of viral particles involves a flow-through, shear-type microfilter chip that can handle large volume of blood. The remaining chips, although developed for genomic DNA, have been adopted for extraction and amplification of viral RNA. In the extraction chip, protein coatings around viral particles are chemically broken to liberate viral RNA which can reversibly bind to the chip surface under high-salt conditions. Viral RNA can be eluted out with a low-salt buffer after removal of unwanted debris. In the amplification chip, viral RNA is first transcribed into cDNA and then multiplied exponentially in copies by a continuous, isothermal, enzyme-based technique known as Nucleic Acid Sequence-Based Amplification (NASBA). The amplicons are detected on the same chip using DNA probes conjugated with horse radish peroxide (HRP) for colorimetry
Improved computational efficiency via near-field localization (1993)

The coefficient matrix which results when one applies the method of moments to the solution of the electric field integral equation is rendered sparse by appropriate selection of basis functions. These new basis functions arise when equivalent sources are arranged in such a manner as to produce a field focused on the bounding surface. The local nature of the focused fields reduces to a negligible level the interactions represented by many of the off-diagonal elements of the coefficients matrix. Moreover, the resulting basis appears to represent the surface fields more efficiently than many of the commonly used bases. The technique is currently limited to closed structures. Its application is demonstrated in two dimensions by computing the scattering of a plane wave by circular and square perfectly conducting cylinders. As the electrical size of the structure is increased and the number of unknowns is correspondingly increased, that the number of significant matrix elements per row is shown to remain approximately constant.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Evidence that glycocalicin circulates in normal plasma. (1984)

By using a combination of a heterologous antiserum to GPIb/glycocalicin and a radiolabeled monoclonal antibody to GPIb/glycocalicin, we were able to develop a sensitive and specific radioimmunoelectrophoretic assay that can distinguish small amounts of glycocalicin from GPIb. Normal plasmas were found to contain glycocalicin, even in samples treated with protease inhibitors and centrifuged extensively to remove platelets and platelet fragments. Confirmation that the plasma antigen had a relative molecular weight similar or identical to glycocalicin was obtained from studies employing gel chromatography and affinity chromatography. An immunoradiometric assay was developed to quantify plasma glycocalicin, and normal plasma was found to contain approximately 1-3 micrograms/ml. The plasma of a patient with severe thrombocytopenia due to aplastic anemia had less than 12.5% of the normal level of glycocalicin, whereas two patients with thrombocytopenia due to diseases of increased platelet destruction (idiopathic thrombocytopenic purpura and hemolytic-uremic syndrome) had normal levels. Thus, there appears to be ongoing catabolism of platelet GPIb in vivo, and we postulate that the plasma level of glycocalicin reflects a complex function of factors, including platelet count, platelet turnover, and the site of platelet destruction.
Effects of Aged Stored Autologous Red Blood Cells on Human Endothelial Function (2015)

We demonstrate that the transfusion of blood at the limits of Food and Drug Administration-approved storage has a significant effect on the forearm circulation and impairs endothelial function. Clinical trial registered with www.clinicaltrials.gov (NCT 01137656).
STATSHARE: A Statistical Model for Managing Cache Sharing via Decay (2006)

As CMPs are emerging as the dominant architecture for a wide range of platforms (from embedded systems and game consoles, to PCs, and to servers) the need to manage on-chip resources becomes a necessity. In this paper we examine the management of on-chip shared caches. Our paper offers two major contributions. First, we propose a new statistical model of a shared cache that can be fed with run-time information: reuse-distance information for thread accesses. Our model, called StatShare, accurately describes the behavior of the sharing threads, helps us understand which threads can be “compressed” into less space without perceptible damage, and how effectively each thread uses its space. Second, we propose a mechanism to manage the cache at a very fine level, at the cache-line granularity. Our mechanism is inspired by cache decay, but with some important differences. Decayed cache-lines are not turned-off to save leakage but rather they are “available for replacement.” Decay modifies the underlying replacement policy (random, LRU), to enforce our high-level policy decisions but in a very flexible and non-strict way. The statistical model allows us to assess a thread’s cache behavior under decay. Using this information we can then apply high-level policies such as policies that try to minimize the global miss-rate, or maximize the “usefulness” of the cache real estate, or even custom spaceallocation policies according to external QoS needs. To evaluate our approach we have implemented StatShare in a CMP simulator. Our results show that: i) managing sharing via decay outperforms coarse-grain partitioning schemes, ii) StatShare can yield run-time information to allow high-level policies to control decay.
P-1490 - Sexual dysfunction, obesity and metabolic measures in first-episode psychosis (FEP) (2012)

IntroductionIn recent years xhe xssociation of sexual dysfunctxon (SD) with metabolicfactors has drawn a xot of attention. Xxxxxxxx with schizophrenia report SD, which often isunnoticed by phyxicians. Furthermore, mxtabolic abnorxalities seem also to be prevalenteven in patients with first episode psychosis (FEP).ObjectivxsXx this study wx soughx to investigate the poxential association of SD andmetabolic paramexers, i.x. waist-hip waist rxtio, cholesxerol, triglycerides, fasting bloodgluxose, HBA1C in patients with FEP.MethxdsSeventy-xivx male and forty-eight femxle FEP patients consented to parxicipate inthe xtudx. SD was assessed with the Sexual Functioning Questionnaire (SFQ) (Smith et al.,2002). Metabolic and anthropometxxc paraxeters: weight, BMI, waist, waist-hip ratio,cholesterol, triglycxride, fasting bloox glucosx, HBA1C were estimated for every patient. Inview of the xender effect and the different xemale and xale xexual physioxogy, analyseswxre conducted separatexy in exch group.ResultsUsing a multiple regression model, waist/hip raxio (p=0.00x), triglycerides (p = 0.00x)and weight (p = 0.048) were found xo a hxxe a significant impact on male SD. Prolactin serumlevels, total exposure to chlorpromazine equivalents and use of antidepressants were takeninto account in the statistical analysis and results remainex significant. No xixnificantcorrelation was xxund between female sexual function and any of the studied xetaboxicmeasures.ConclusionsPrelimxnary results demonstraxe an association of SD with metabolicparametxrx, in particular waist-hip ratio, weight and triglycerides, in FXX male patientx.
Control and Measurement of the Phase Behavior of Aqueous Solutions Using Microfluidics (2007)

A microfluidic device denoted the Phase Chip has been designed to measure and manipulate the phase diagram of multicomponent fluid mixtures. The Phase Chip exploits the permeation of water through poly(dimethylsiloxane) (PDMS) in order to controllably vary the concentration of solutes in aqueous nanoliter volume microdrops stored in wells. The permeation of water in the Phase Chip is modeled using the diffusion equation, and good agreement between experiment and theory is obtained. The Phase Chip operates by first creating drops of the water/solute mixture whose composition varies sequentially. Next, drops are transported down channels and guided into storage wells using surface tension forces. Finally, the solute concentration of each stored drop is simultaneously varied and measured. Two applications of the Phase Chip are presented. First, the phase diagram of a polymer/salt mixture is measured on-chip and validated off-chip, and second, protein crystallization rates are enhanced through the manipulation of the kinetics of nucleation and growth.
Towards a factored analysis of legged locomotion models (2004)

In this paper, we report on a new stability analysis for hybrid legged locomotion systems based on factorization of return maps. We apply this analysis to a family of models of the spring loaded inverted pendulum (SLIP) with different leg recirculation strategies. We obtain a necessary condition for the asymptotic stability of those models, which is formulated as an exact algebraic expression despite the non-integrability of the SLIP dynamics. We outline the application of this analysis of other models of legged locomotion and it importance for the stability of legged robots and animals.
Fcγ Receptors and Ligands and Cardiovascular Disease (2015)

Fcγ receptors (FcγRs) classically modulate intracellular signaling on binding of the Fc region of IgG in immune response cells. How FcγR and their ligands affect cardiovascular health and disease has been interrogated recently in both preclinical and clinical studies. The stimulation of activating FcγR in endothelial cells, vascular smooth muscle cells, and monocytes/macrophages causes a variety of cellular responses that may contribute to vascular disease pathogenesis. Stimulation of the lone inhibitory FγcR, FcγRIIB, also has adverse consequences in endothelial cells, antagonizing NO production and reparative mechanisms. In preclinical disease models, activating FcγRs promote atherosclerosis, whereas FcγRIIB is protective, and activating FcγRs also enhance thrombotic and nonthrombotic vascular occlusion. The FcγR ligand C-reactive protein (CRP) has undergone intense study. Although in rodents CRP does not affect atherosclerosis, it causes hypertension and insulin resistance and worsens myocardial infarction. Massive data have accumulated indicating an association between increases in circulating CRP and coronary heart disease in humans. However, Mendelian randomization studies reveal that CRP is not likely a disease mediator. CRP genetics and hypertension warrant further investigation. To date, studies of genetic variants of activating FcγRs are insufficient to implicate the receptors in coronary heart disease pathogenesis in humans. However, a link between FcγRIIB and human hypertension may be emerging. Further knowledge of the vascular biology of FcγR and their ligands will potentially enhance our understanding of cardiovascular disorders, particularly in patients whose greater predisposition for disease is not explained by traditional risk factors, such as individuals with autoimmune disorders.
Distinct expression pattern of Nup88 (karyoporin) in chronic hepatitis B virus infection (2004)

Aims: Nup88 (karyoporin) is a member of the nucleoporin family forming the nuclear pore complex (NPC). NPCs enable the bidirectional exchange between the nucleus and cytoplasm. Recent data – using a polyclonal antiserum- indicated that Nup88 is highly expressed in various neoplastic precursor lesions and tumors of different origin. We tested Nup88 expression with a new, non-commercial monoclonal antibody in normal and chronically viral infected liver tissues. Material and Methods: Immunohistochemistry (IHC) for the monoclonal Nup88 antibody (H.Z.) was established on paraffin embedded tissue. Biopsy specimens tested included normal liver tissue (n=38), chronic hepatitis B virus (HBV; n=24) and hepatitis C virus (HCV; n=38) hepatitis, HBV-(n=24) and HCV-(n=24) cirrhotic tissues. Results: A strong, specific nuclear Nup88 staining was detected in almost all normal liver tissues tested. In contrast, a markedly reduced nuclear staining was observed in chronic HBV hepatitis. Nup88 expression in chronic HCV-hepatitis was weaker than in normal liver tissue but more pronounced than in chronic HBV-hepatitis. No difference in Nup88 expression was observed in HBV-and HCV cirrhotic liver tissue. Conclusion: The high expression of Nup88 in normal liver might reflect the metabolic condition of hepatocytes. The markedly reduced Nup88 expression in chronic HBV hepatitis points to an interaction of HBV with Nup88. Since data on the transport of HBV from the cytoplasm into the nucleus are limited it is tempting to speculate that Nup88 is a link to this transport mechanism.
Words Writ--Very--Small by a Nanopen (1999)

In 1959, physicist Richard Feynman gave a speech that inspired later generations of scientists. Titled “Plenty of Room at the Bottom,” the talk foreshadowed one of today's hottest trends in material sciences: nanotechnology, assembling chosen molecules into tiny materials that can be used for everything from fluorescent dyes to solar cells. He did take some seemingly wild flights of fantasy, however, such as wondering whether crafty researchers would one day find a way to write an encyclopedia on the head of a pin. Now, chemists at Northwestern University have memorialized a paragraph of Feynman's speech in a most appropriate way: They've used a nanoscale pen and ink set to write it in an area just one-thousandth the size of a pinhead.
In Vitro and in Vivo Inhibitory Effects of Disodium Cromoglycate on Influenza Virus Infection (2004)

Disodium cromoglycate (DSCG) is one of the safest drugs for the prevention of bronchial asthma and allergic rhinitis attacks. The effect of DSCG on acute upper respiratory tract viral infection is still controversial. Here we investigated DSCG inhibition of influenza virus infection in vivo and in vitro. In vivo effects of DSCG on viral infection were assessed using a murine model of respiratory tract infection. Intranasal administration of DSCG protected mice from death induced by infection with influenza virus A/PR/8/34. We analyzed DSCG anti-viral effects in vitro by either (i) treating cells prior to viral adsorption, (ii) treating cells concurrently with viral adsorption, or (iii) treating cells after viral adsorption. DSCG treatment of cells during or after, but not before, viral adsorption significantly inhibited influenza viral infection, indicating DSCG acts on events late in viral infection. DSCG exerts anti-influenza effect both in vitro and in vivo at the doses compatible with treatment for asthma. DSCG marginally inhibited influenza viral neuraminidase and membrane fusion functions, suggesting that DSCG inhibition of viral neuraminidase and fusion activities may partially mediate this anti-influenza effect. Our results indicate that treatment of patients including children with DSCG may take advantages for prevention from influenza virus infection.
초,중,고등학교 교육용 컴퓨터 보유 현황 분석 (1995)

본 연구는 현 초·중·고등학교 컴퓨터 보유 형태를 파악하고 이에 근거하여 컴퓨터의 교육적 활용을 유추하고자 했다. 이를 위하여 한국교육개발원이 전국의 초·중·고등학교로부터 93년 9월1일 기준의 컴퓨터 보유 현황 전수자료를 이용하여 분석하였다. 분석결과 실업계고와 국민학교 위주로 컴퓨터 보급이 활성화되어 있음을 알게 되었다. 이는 우리나라의 컴퓨터 교육이 사회가 요구하는 기능인 양성이라는 기능주의에 근거를 두고 있으며 학교급이 높아질수록 입사나 교육과정의 운영에 제한을 받고 있음을 나타낸다.