gebhart/patrepeval huggingface dataset:
Relaxed space switch crosstalk requirements: crosstalk analysis in the presence of laser wavelength referencing error and drift (2002)

We present for the first time analytical expressions characterizing homodyne beat noise effects in the presence of laser wavelength referencing error and drift, and show that for typical referencing error and drift values the crosstalk requirements for large space switches in optical cross-connects can be relaxed by up to fourteen decibels.
Plant Regeneration via Somatic Embryogenesis from Cotyledon Protoplasts of Tomato(Lycopersicon esculentum Mill.) (1994)

Plant regeneration from cotylendon protoplasts of tomato (Lycopersicon esculentum cv. Kyoryokutoko) was obtained by somatic embryogenesis. Protoplasts were isolated from cotyledons of 8-10 day old in vitro-grown seedlings raised in a controlled chamber (20 °C, 3, 000-4, 500 lux, 16h). During initial protoplast culture, the addition of TM-2 medium (Shahin 1985) containing 0.15M sucrose, at 4 day intervals (sucrose concentrations were progressively reduced, 0.2M-0.17M-0.16M), resulted in vigorous cell divisions and prevented the browning of the cells. Somatic embryogenesis was induced from up to 50% of selected greenish calli when cultured on TM-4 medium supplemented with 2.0 mg/l zeatin riboside. Multiple adventitious shoots were formed by clonal propagation of somatic embryos on TM-4 or Murashige and Skoog (1962) medium containing 1.0 mg/l zeatin and 0.1 mg/l indoleacetic acid. Ninety-eight regenerated plants obtained from rooting shoots on Murashige and Skoog medium (0.8% agar, 20 g/l sucrose) were grown to maturity.
Computer-Assisted Color Classification of Peanut Pods1 (2002)

Abstract Mesocarp color classification in the Hull Scrape Maturity Method is the most important step in determining peanut maturity and optimum harvest date. This research involved the development of an image acquisition system and a software procedure for color classification. Images of peanut pods that had been sorted manually into color classes and subclasses were used in computer training. After training with sorted color classes, the computer-assisted procedure correctly identified and classified the peanut pods, with a 99% precision for the same sample in different alignment, and with a 95% precision for different size samples taken from the same population. Pod size measurement with a resolution of 0.1 mm was also performed using image processing techniques.
Disruption of hyaluronan synthase-2 abrogates normal cardiac morphogenesis and hyaluronan-mediated transformation of epithelium to mesenchyme (2000)

We identified hyaluronan synthase-2 (Has2) as a likely source of hyaluronan (HA) during embryonic development, and we used gene targeting to study its function in vivo. Has2(-/-) embryos lack HA, exhibit severe cardiac and vascular abnormalities, and die during midgestation (E9.5-10). Heart explants from Has2(-/-) embryos lack the characteristic transformation of cardiac endothelial cells into mesenchyme, an essential developmental event that depends on receptor-mediated intracellular signaling. This defect is reproduced by expression of a dominant-negative Ras in wild-type heart explants, and is reversed in Has2(-/-) explants by gene rescue, by administering exogenous HA, or by expressing activated Ras. Conversely, transformation in Has2(-/-) explants mediated by exogenous HA is inhibited by dominant-negative Ras. Collectively, our results demonstrate the importance of HA in mammalian embryogenesis and the pivotal role of Has2 during mammalian development. They also reveal a previously unrecognized pathway for cell migration and invasion that is HA-dependent and involves Ras activation.
Donor-Acceptor Distance Distributions in a Double-Labeled Fluorescent Oligonucleotide Both as a Single Strand and in Duplexes (1995)

A 16-mer deoxyribonucleotide was labeled at the 5'-end with x-rhodamine and at the 3'-end with fluorescein. Förster resonance energy transfer was used to determine the distribution, P(R), of donor-acceptor distances for the oligomer in three duplex structures (hybridized to complementary strands having 10, 16, and 24 bases) and as a single strand; measurements were made in 0.18 M NaCl and 1 M KCl solutions. These distributions were derived from lifetime measurements made in the frequency domain using a multiharmonic frequency phase fluorometer. The fluorescein fluorescence decay for each duplex structure was fit very well with P(R) modeled as a shifted Gaussian. On the basis of the mean donor-acceptor distance, these structures for the 16-mer and the 24-mer were consistent with that of B-DNA. For the 16-mer duplex in 0.18 M NaCl, the distribution was centered at 68.4 A with sigma = 6.4 A. For the 10-mer duplex, the mean donor-acceptor distance, 61.8 A, is much larger than that for a structure with the fluorophores extended perpendicular to the helix axis. For the single-strand data, various high-quality fits yielded physically unreasonable distributions or could not accurately account for the acceptor response. Considered analyses suggested that the single-strand distribution was best represented by a shifted Gaussian, with R = 51.5 A and sigma = 10.0 A in 0.18 M NaCl. In all cases, sigma increased and R decreased in 1 M KCl relative to their values in 0.18 M NaCl, consistent with the increased flexibility of the polymer.
Nanoparticles in the ocular drug delivery. (2013)

Ocular drug transport barriers pose a challenge for drug delivery comprising the ocular surface epithelium, the tear film and internal barriers of the blood-aqueous and blood-retina barriers. Ocular drug delivery efficiency depends on the barriers and the clearance from the choroidal, conjunctival vessels and lymphatic. Traditional drug administration reduces the clinical efficacy especially for poor water soluble molecules and for the posterior segment of the eye. Nanoparticles (NPs) have been designed to overcome the barriers, increase the drug penetration at the target site and prolong the drug levels by few internals of drug administrations in lower doses without any toxicity compared to the conventional eye drops. With the aid of high specificity and multifunctionality, DNA NPs can be resulted in higher transfection efficiency for gene therapy. NPs could target at cornea, retina and choroid by surficial applications and intravitreal injection. This review is concerned with recent findings and applications of NPs drug delivery systems for the treatment of different eye diseases.
The EAP-TLS Authentication Protocol (2008)

This document specifies an Internet standards track protocol for the Internet community, and requests discussion and suggestions for improvements.
Mammalian capping enzyme complements mutant Saccharomyces cerevisiae lacking mRNA guanylyltransferase and selectively binds the elongating form of RNA polymerase II (1997)

5'-Capping is an early mRNA modification that has important consequences for downstream events in gene expression. We have isolated mammalian cDNAs encoding capping enzyme. They contain the sequence motifs characteristic of the nucleotidyl transferase superfamily. The predicted mouse and human enzymes consist of 597 amino acids and are 95% identical. Mouse cDNA directed synthesis of a guanylylated 68-kDa polypeptide that also contained RNA 5'-triphosphatase activity and catalyzed formation of RNA 5'-terminal GpppG. A haploid strain of Saccharomyces cerevisiae lacking mRNA guanylyltransferase was complemented for growth by the mouse cDNA. Conversion of Lys-294 in the KXDG-conserved motif eliminated both guanylylation and complementation, identifying it as the active site. The K294A mutant retained RNA 5'-triphosphatase activity, which was eliminated by N-terminal truncation. Full-length capping enzyme and an active C-terminal fragment bound to the elongating form and not to the initiating form of polymerase. The results document functional conservation of eukaryotic mRNA guanylyltransferases from yeast to mammals and indicate that the phosphorylated C-terminal domain of RNA polymerase II couples capping to transcription elongation. These results also explain the selective capping of RNA polymerase II transcripts.
An Expanded Two-State Model Accounts for Homotropic Cooperativity in Biosynthetic Threonine Deaminase from Escherichia coli (1995)

The linkage between substrate and regulatory effector binding to separate sites on allosteric enzymes results in shifts in their sigmoidal kinetics to regulate metabolism. Control of branched chain amino acid biosynthesis in Escherichia coli occurs in part through shifts in the sigmoidal dependence of alpha-ketobutyrate production promoted by isoleucine and valine binding to biosynthetic threonine deaminase. The structural similarity of threonine, valine, and isoleucine have given rise to suggestions that there may be competition among different ligands for the same sites on this tetrameric enzyme, resulting in a complex pattern of regulation. In an effort to provide a coherent interpretation of the cooperative association of ligands to the active sites and to the effector sites of threonine deaminase, binding studies using single amino acid variants were undertaken. A previously-isolated, feedback-resistant mutant identified in Salmonella typhimurium, ilvA219, has been cloned and sequenced. The phenotype is attributable to a single amino acid substitution in the regulatory domain of the enzyme in which leucine at position 447 is substituted with phenylalanine. The mutant exhibits hyperbolic saturation curves in both ligand binding and steady-state kinetics. These results, in addition to calorimetric and spectroscopic measurements of isoleucine and valine binding, indicate that the low affinity (T) state is destabilized in the mutant and that it exists predominantly in the high affinity (R) conformation in the absence of ligands, providing an explanation for its resistance to isoleucine. Chemical and spectroscopic analyses of another mutant, in which alanine has replaced an essential lysine at position 62 that forms a Schiff base with pyridoxal phosphate, indicate that the cofactor is complexed to exogenous threonine and is therefore unable to bind additional amino acids at the active sites. Isoleucine and valine binding to this inactive, active site-saturated enzyme revealed that it too was stabilized in the R state, yielding binding constants in excellent agreement with the leucine to phenylalanine mutant. The lysine to alanine mutant was further utilized to demonstrate that both threonine and 2-aminobutyrate bind with stronger affinity to the regulatory sites than to the active sites. A direct consequence of these results is that substrates and analogs have a synergistic effect on the allosteric transition since, in effect, they act as both homotropic and heterotropic effectors.(ABSTRACT TRUNCATED AT 250 WORDS)
Advances in domain independent linear text segmentation (2000)

This paper describes a method for linear text segmentation which is twice as accurate and over seven times as fast as the state-of-the-art (Reynar, 1998). Inter-sentence similarity is replaced by rank in the local context. Boundary locations are discovered by divisive clustering.
In Vitro Evaluation of Chemically Cross-Linked Shape-Memory Acrylate−Methacrylate Copolymer Networks as Ocular Implants (2010)

Acrylates have been used in ophthalmic practice as a paradigmatic implant material for decades, especially as intraocular lens for their excellent transparency. A novel polymeric shape memory system of chemically cross-linked acrylate-methacrylate copolymer networks was developed and characterized in this study. The thermomechanical properties, shape memory properties, transparency, and surface wettability as well as cytotoxicity were systematically evaluated to mimic the in vivo situation by differential scanning calorimetry (DSC), tensile tests, spectrophotometer, Abbe refractometer, contact angle measurements, and MTT assay. It was found that the chemically cross-linked copolymer network behaves as an elastomer capable of arbitrary shaping above the glass-transition temperature. Transition temperatures of the networks were tunable through the change of the composition of monomers.
A new synthesis of indolizines via thermal cyclisation of 2-pyridyl derivatives (1990)

M. L. Bode and P. T. Kaye, J. Chem. Soc., Perkin Trans. 1, 1990, 2612 DOI: 10.1039/P19900002612
Physical Activity Promotion: Experiences and Evaluation of the Agita São Paulo Program Using the Ecological Mobile Model (2004)

A sedentary lifestyle, which affects 50–80% of the world’s population, is associated with the main risk factors for chronic diseases. For this reason stimulating an active lifestyle is critical to control and prevent these illnesses. In Brazil, as in other developing countries, there are few available data to determine the physical activity (PA) level of the population. The first national household survey in Brazil to include PA was the Living Standards Measurement Survey conducted from March 1996 to February 1997. In that survey 11,033 people (over 20 years of age) were evaluated concerning their leisure-time physical activities (LTPA). According to the results only 13% of the Brazilians surveyed reported performing 30 min or more of LTPA on 1 or more days of the week, and only 3.3% reported doing the recommended minimum of 30 min on 5 or more days of the week. In the State of Sao Paulo, data published in 1990 showed that a sedentary lifestyle was prevalent among 69.3% males and females (aged 18 to 70). Another important issue is the cost of this lifestyle in the health system, which is estimated by the Centers for Disease Control and Prevention (CDC) to represent about 70% of all health expenses. According to 2002 data (CELAFISCS & CDC, unpublished data), 4% (US$37.5 million) of the direct cost in public health in the State of Sao Paulo is related to a sedentary lifestyle. The Agita Sao Paulo Program (the Program) was developed to address this problem. The Program is a multilevel plan that promotes messages about the health benefits of PA and coordinates activities and interventions for broader PA opportunities among more than 37 million inhabitants of the Journal of Physical Activity and Health, 2004, 1, 81-97 © 2004 Human Kinetics Publishers, Inc.
On-Demand Resource Provisioning for BPEL Workflows Using Amazon's Elastic Compute Cloud (2009)

BPEL is the de facto standard for business process modeling in today's enterprises and is a promising candidate for the integration of business and Grid applications. Current BPEL implementations do not provide mechanisms to schedule service calls with respect to the load of the target hosts. In this paper, a solution that automatically schedules workflow steps to underutilized hosts and provides new hosts using Cloud computing infrastructures in peak-load situations is presented. The proposed approach does not require any changes to the BPEL standard. An implementation based on the ActiveBPEL engine and Amazon's Elastic Compute Cloud is presented.
Osteoporosis in Men with Idiopathic Hypogonadotropic Hypogonadism (1987)

To assess the effect of testosterone deficiency on skeletal integrity in men, we determined bone density in 23 hypogonadal men with isolated gonadotropin-releasing hormone deficiency and compared those values with ones from controls. Cortical bone density, as assessed by single-photon absorptiometry of the nondominant radius, ranged from 0.57 to 0.86 g/cm2 (mean +/- SE, 0.71 +/- 0.02) in patients with fused epiphyses and from 0.57 to 0.67 g/cm2 (mean, 0.61 +/- 0.01) in patients with open epiphyses, both of which were significantly (p less than 0.001) lower than normal. Spinal trabecular bone density, as assessed by computed tomography, was similarly decreased (p less than 0.0001) and ranged from 42 to 177 mg K2HPO4/cm3 (mean, 112 +/- 7). Cortical bone density was at least 2 SD below normal in 16 of 23 men, and 8 men had spinal bone densities below the fracture threshold of 80 to 100 mg K2HPO4/cm3. Osteopenia was equally severe in men with immature and mature bone ages, suggesting that abnormal bone development plays an important role in the osteopenia of men with idiopathic hypogonadotropic hypogonadism.
Fibronectin Isoform Distribution in the Mouse II. Differential Distribution of the Alternatively Spliced EIIIB, EIIIA, and V Segments in the Adult Mouse (1996)

The alternatively spliced EIIIB, EIIIA, and V segments of fibronectin (FN) show widespread codistribution in the mouse embryo, suggesting that EIIIB+, EIIIA+, and V+ isoforms serve to facilitate morphogenesis and organogenesis (Peters, JH, and Hynes, RO, 1996, this issue). To gain further clues to functions of these segments, we have used segment-specific anti-FN antibodies to perform immunofluorescence microscopy on tissue sections obtained from mice aged 9 to 15 weeks. Staining for each of the three spliced segments, relative to that for the total FN pool, was reduced in the adult as compared with the embryo. Anti-V antibodies produced patterns which were most similar to those obtained with anti-total FN antibodies, localizing to basement membranes, connective tissues subjacent to epithelia, walls of blood vessels, and cartilage. Anti-EIIIA antibodies produced the next most widespread pattern, which included prominent staining of the walls of blood vessels of all sizes, the lung interstitium, and smooth muscle associated with the gastrointestinal (GI), genitourinary (GU), and respiratory tracts. Although anti-EIIIB antibodies produced the faintest and most restricted pattern of staining, EIIIB+ FN could be detected in the walls of some smaller blood vessels, smooth muscle of the GI, GU, and respiratory tracts, as well as within cartilaginous structures, and eye. There were quantitative and/or qualitative differences in the staining patterns produced by the three segment-specific antibodies in a variety of tissues, including liver, cartilage, synovium, cornea, muscle, peripheral nerve, and lymph node. These findings suggest that each of the spliced segments of the FN molecule may occupy unique physical or functional positions within the extracellular matrix of the adult mouse.
The Potential Use of N-Myristoyltransferase as a Biomarker in the Early Diagnosis of Colon Cancer (2011)

Colon cancer is one of the most common malignant diseases and a major cause of mortality in the Western world. Metastasis to lymph nodes and other gastrointestinal organs, especially to the liver and lungs, is most common and occurs in up to 25% of cancer patients when initially diagnosed. The majority of colon cancers develop from noncancerous adenomatous polyps on the lining of the colon which grow over the years to become cancerous. If detected early, the surgical resections of the growth, often in combination with chemotherapy, significantly increases life expectancy. We have shown that the enzyme N-myristoyltransferase (NMT) which carries out lipid modification of several proteins (including many of those involved in oncogenesis) is expressed at higher levels in cancerous tissues from the colon. We have also shown that in peripheral blood mononuclear cells (PBMC) and bone marrow (BM) cells collected from colon cancer patients and from azoxymethane-induced rats the expression and localization of NMT is altered. We have observed strong positivity for NMT in immunohistochemical analysis for PBMC from colon cancer patients as compared to control groups. Furthermore, in the bone marrow (BM) mononuclear cells, NMT was found to be confined to the nuclei whereas in control groups it was observed to be located in the cytoplasm. In conclusion, this strikingly differential localization offers the basis of a potential investigational tool for screening or diagnosis of individuals at risk for or suspected of having colon cancer.
Theory of Dislocations (1968)

Dislocations in Isotropic Continua. Effects of Crystal Structure on Dislocations. Dislocation-Point-Defect Interactions at Finite Temperatures. Groups of Dislocations. Appendixes. Author and Subject Indexes.
Preparation of Pb(Zr,Ti)O3 Thin Films by Sol-Gel Technique (1994)

In the preparation of P b ( Z r , T i ) O 3 thin films by the sol-gel technique, the following procedures are involved: evaporation of solvents, thermal decomposition of organometallic compounds and crystallization. In this study, a thermodynamic approach was employed for the sol-gel technique, and the processing conditions were determined. For the processes of evaporation and thermal decomposition, the processing conditions were determined by applying Ozawa's method [Bull. Chem. Soc. Jpn. 38 (1965) 1881]. By means of both the X-ray diffraction technique and scanning electron microscope technique, crystal structure and surface morphology were evaluated. It was observed that the perovskite phase began to form at 500° C and was completed at 600° C. Moreover, a dense Pb(Zr,Ti)O 3 thin film was also formed by annealing at 700° C for 120 min.
Cell Adhesion and Short-Term Patency in Human Endothelium Preseeded 1.5-mm Polytetrafluoroethylene Vascular Grafts: An Experimental Study (1997)

It has been shown that endothelialization improves short-term patency of 1.5-mm expanded polytetrafluoroethylene vascular grafts. A model for endothelialization of 1.5-mm expanded polytetrafluoroethylene vascular grafts with human endothelial cells is described. In this model, the adherence of endothelial cells was increased significantly in grafts coated with serum proteins and collagen. By means of this protocol, graft patency was tested after implantation in two animal models: the rat aorta and the rabbit common carotid artery. Anastomosis was performed with a 3M Precise Microvascular Anastomotic System. In both animal models, no significant loss of endothelial cells in the precoated grafts (rat, n = 8) were noted 1 hour after blood flow restoration. All uncoated grafts showed significant endothelial cell loss. In the rabbit model, all nonendothelialized grafts (n = 8) clotted 5 to 25 minutes after flow restoration. Seven (n = 8) endothelialized grafts showed no clotting during 1 hour's observation: one clotted immediately for a patency rate of 87.5 percent. These results indicate that endothelialization of 1.5-mm grafts is practical. Furthermore, adhesion of endothelial cells to the graft walls is not affected by short-term, pulsatile, high-pressure blood flow.
The combination of oral acitretin and bath PUVA for the treatment of severe psoriasis (1997)

Journal Article The combination of oral acitretin and bath PUVA for the treatment of severe psoriasis Get access S. MUCHENBERGER, S. MUCHENBERGER Photodermatology Unit, Department of Dermatology, University Medical Centre, Freiburg, Germany Search for other works by this author on: Oxford Academic Google Scholar E. SCHöPF, E. SCHöPF Photodermatology Unit, Department of Dermatology, University Medical Centre, Freiburg, Germany Search for other works by this author on: Oxford Academic Google Scholar J.C. SIMON J.C. SIMON Photodermatology Unit, Department of Dermatology, University Medical Centre, Freiburg, Germany J.C. Simon MD, Department of Dermatology, Hauptstraβe 7, D‐79104 Freiburg, Germany. Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 137, Issue 4, 1 October 1997, Pages 587–589, https://doi.org/10.1111/j.1365-2133.1997.tb03791.x Published: 01 October 1997 Article history Accepted: 16 May 1997 Published: 01 October 1997
The Macroheterogeneity of Type I Tyrosine-rich Proteins of Merino Wool (1974)

The cystine-poor family of tyrosine-rich proteins of wool as their S-carboxymethyl derivatives has been shown by chromatography on quaternary ammonium ethylcellulose (QAE-cellulose) at pH 10· 5 to consist of 10 groups of proteins which span a threefold range of tyrosine content and vary in many other amino acids, particularly S-carboxymethylcysteine. Electrophoresis at pH 8·9 revealed that most fractions contain two or more components and by further chromatography of five of these groups at pH 8·3 on DEAE-cellulose 10 components have been isolated. The amino acid composition suggests that the components within each QAE-cellulose group are very closely related.