gebhart/patrepeval huggingface dataset:
Cloning and Sequencing of cDNAs Coding for the Human Intra-Acrosomal Antigen SP-101 (1990)

cDNAs coding for the intra-acrosomal protein SP-10 were cloned and characterized as a first step in understanding the expression of this antigen during spermatogenesis. Three overlapping SP-10-specific cDNAs were isolated from a human testes cDNA expression library. These cDNAs hybridized to a 1.35-kb mRNA that was present in human testes but was not found in liver or placenta. Complete sequencing of these cDNAs, designated SP-10-5, SP-10-8, and SP-10-10, produced an 1117-bp sequence containing a 265-amino acid-coding region for the SP-10 protein. Hydrophobicity plots generated from the deduced amino acid sequence showed a very hydrophobic amino terminus characteristic of a signal peptide. Sequence data showed that three different amino acid repeats occurred a total of 16 times in the central third of the SP-10 protein. Interestingly, cDNA SP-10-10 has an internal 57-base pair (19 amino acids) in-frame deletion that is not present in SP-10-5, suggesting that alternative splicing generates more than one SP-10 mRNA. The SP-10 protein appears to be a unique acrosomal protein, based on previous immunohistological data and the observation that SP-10 cDNA sequences did not show any significant homology to other sequences found in the Genbank, National Biomedical Research Foundation, or Swiss sequence banks. A recombinant SP-10 fusion protein was produced in an Escherichia coli expression vector and used to generate a polyclonal antiserum. This antiserum stained the acrosomal cap in situ and reacted with a similar set of peptides on Western blots as did a monoclonal antibody to SP-10.
The Scholarly Vocation and Library Science (1991)

The author analyzes the scholarly productivity of library school faculty as compared to other disciplines, and poses some hard questions as well as some hopeful answers
Neutralization of the AIDS Retrovirus by Antibodies to a Recombinant Envelope Glycoprotein (1986)

Mammalian cell lines have been engineered to produce a secreted form of the AIDS retrovirus envelope glycoprotein. The recombinant protein has been isolated from growth-conditioned culture media and used to immunize animals. Antibodies directed against the recombinant molecule were found to react with the envelope glycoprotein produced in virus-infected cells. Furthermore, these antibodies were able to directly inactivate the AIDS retrovirus in a neutralization assay in vitro. The expression system reported here should provide sufficient quantities of the AIDS retrovirus envelope protein for biological and vaccination studies.
SLM peak-power reduction without explicit side information (2001)

Selected mapping (SLM) peak-power reduction is distortionless as it selects the actual transmit signal from a set of alternative signals, which all represent the same information. The specific signal generation information needs to be transmitted and carefully protected against bit errors. Here, me propose an extension of SLM, which employs scrambling and refrains from the use of explicit side information in the receiver. Some additional complexity and nearly vanishing redundancy is introduced to achieve markedly improved transmit signal statistics. Even though SLM is applicable with any modulation, we concentrate on orthogonal frequency-division multiplexing (OFDM) in this letter.
A new and efficient asymmetric synthesis of 1‐amino‐1‐alkylphosphonic acids (2000)

A new and efficient method for the asymmetric synthesis of 1-amino-1-alkyl phosphonic acids is described. It involves a diastereoselective alkylation of a bicyclic chloromethyl phosphonamide derived from (S)-2-anilinomethylpyrrolidine, with subsequent conversions leading to an amino compound by the Staudinger method. Acid hydrolysis affords the target molecule of high enantiomeric purity and good yield. © 2000 John Wiley & Sons, Inc. Heteroatom Chem 11:528–535, 2000
Fast and robust blind-equalization based on cyclic prefix (2003)

The cyclic prefix is commonly used in the context of frequency domain equalization in DMT channels. We observe that it can be used in more general contexts and show its advantages in blind equalization, especially for non-minimum phase channels.
Data collection handbook to support modeling the impacts of radioactive material in soil (1993)

A pathway analysis computer code called RESRAD has been developed for implementing US Department of Energy Residual Radioactive Material Guidelines. Hydrogeological, meteorological, geochemical, geometrical (size, area, depth), and material-related (soil, concrete) parameters are used in the RESRAD code. This handbook discusses parameter definitions, typical ranges, variations, measurement methodologies, and input screen locations. Although this handbook was developed primarily to support the application of RESRAD, the discussions and values are valid for other model applications.
Dendritic Cell Vaccines for Cancer Stem Cells (2009)

Accumulating evidence suggests that only a fraction of neoplastic cells, defined as cancer stem cells (CSC), are responsible for tumor perpetuation. Recent data suggest that neurospheres (NS) from glioblastoma multiforme (GBM) are enriched in CSC. The characterization of this subpopulation of brain tumor cells with a potent tumorigenic activity supports the cancer stem cell hypothesis in solid tumors and may imply that cancer cells are differentially targeted by treatments, including dendritic cell (DC) immunotherapy. To test therapeutic strategies, a good model mimicking the characteristics of GBM-NS and GBM-AC (Adherent Cells) was necessary. One of the most frequently used murine brain tumor models is the GL261 glioma cell line. To see whether GL261 cells could mimic the growth of human GBM-CSC we let them grow in EGF/bFGF without serum. After 5 days neurospheres were visible in the culture medium and were proliferating continuously. The characterization in vivo and in vitro demonstrates that GL261-NS satisfy criteria used to identify CSC and are more immunogenic than AC. DC loaded with GL261-NS lysates protect mice against tumors from both GL261-NS and GL261-AC. Our results suggest that only DC vaccination against neurospheres can restrain the growth of a highly infiltrating and aggressive model of glioma and may have implications for the design of novel, more effective immunotherapy trials for malignant glioma and possibly other malignancies.
Cyclic AMP regulates somatostatin mRNA accumulation in primary diencephalic cultures and in transfected fibroblast cells (1986)

Although the factors controlling the secretion of the neuropeptide somatostatin have been extensively studied, little is known about the mechanisms that control somatostatin biosynthesis. Somatostatin secretion is regulated by numerous agents that increase intracellular levels of cAMP. We sought to determine whether cAMP also regulates somatostatin mRNA accumulation. We found that forskolin elicited an increase in somatostatin secretion and mRNA levels in primary cultures of rat diencephalic cells. Another secretagogue, KCl, was as effective as forskolin in causing somatostatin secretion but had no effect on mRNA accumulation. Somatostatin expression in fibroblast cells transfected with the somatostatin gene was also regulated by forskolin. These results demonstrate that somatostatin mRNA accumulation can be regulated through a cAMP-dependent pathway, that this pathway is operative in heterologous cells transfected with the somatostatin gene, and that stimulation of somatostatin secretion and mRNA accumulation can be uncoupled from one another.
Endoplasmic Reticulum Stress Signaling in Disease (2006)

The extracellular space is an environment hostile to unmodified polypeptides. For this reason, many eukaryotic proteins destined for exposure to this environment through secretion or display at the cell surface require maturation steps within a specialized organelle, the endoplasmic reticulum (ER). A complex homeostatic mechanism, known as the unfolded protein response (UPR), has evolved to link the load of newly synthesized proteins with the capacity of the ER to mature them. It has become apparent that dysfunction of the UPR plays an important role in some human diseases, especially those involving tissues dedicated to extracellular protein synthesis. Diabetes mellitus is an example of such a disease, since the demands for constantly varying levels of insulin synthesis make pancreatic beta-cells dependent on efficient UPR signaling. Furthermore, recent discoveries in this field indicate that the importance of the UPR in diabetes is not restricted to the beta-cell but is also involved in peripheral insulin resistance. This review addresses aspects of the UPR currently understood to be involved in human disease, including their role in diabetes mellitus, atherosclerosis, and neoplasia.
A vectorization technique of hashing and its application to several sorting algorithms (2002)

A vectorized algorithm for entering data into a hash table is presented. A program that enters multiple data could not be executed on vector processors by conventional vectorization techniques because of data dependences. The proposed method enables execution of multiple data entry by conventional vector processors and improves the performance by a factor of 12.7, compared with the normal sequential method, when 4099 pieces of data are entered on the Hitachi S-810. This method is applied to address calculation sorting and the distribution counting sort, whose main part was unvectorizable by previous techniques. It improves performance by a factor of 12.8 when n=2/sup 14/ on the S-810.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
The vagaries of variegating transgenes (1996)

Expression of transgenes in mice, when examined with assays that can distinguish individual cells, is often found to be heterocellular, or variegated. Line-to-line variations in expression of a transgene may be due largely to differences in the proportion of cells in which it is expressed. Variegated silencing by centromeric heterochromatin is well described, but other factors may also affect transgene silencing in mice. Tandem arrays of transgenes themselves form heterochromatin, and some cell lineages may tend to silence transgenes because of extensive facultative heterochromatin in their nuclei. The cis-acting transcriptional control elements within a transgene inhibit silencing, and strain-specific differences in chromatin proteins may strongly influence the extent of variegation. The accessibility of multiple differentiated cell lineages in mice suggests that they may provide a tool for dissecting the role of chromatin-mediated silencing in cell differentiation and tissue-specific gene expression.
Communications among data and science centers (1990)

The ability to electronically access and query the contents of remote computer archives is of singular importance in space and earth sciences; the present evaluation of such on-line information networks' development status foresees swift expansion of their data capabilities and complexity, in view of the volumes of data that will continue to be generated by NASA missions. The U.S.'s National Space Science Data Center (NSSDC) manages NASA's largest science computer network, the Space Physics Analysis Network; a comprehensive account is given of the structure of NSSDC international access through BITNET, and of connections to the NSSDC available in the Americas via the International X.25 network.
Plasma adiponectin is decreased in nonalcoholic fatty liver disease (2005)

These data support a role for low circulating adiponectin in the pathogenesis of NAFLD and confirm the strict association between reduced adiponectin production by adipose tissue, NAFLD and insulin resistance.
Mouse/human chimeric monoclonal antibody in man: kinetics and immune response. (1989)

A mouse/human chimeric monoclonal antibody (mAb) composed of the variable regions of murine 17-1A mAb and the constant regions of human IgG-1K immunoglobulin was administered to 10 patients with metastatic colon cancer. Four patients received single infusion of 10 mg (n = 2) or 40 mg (n = 2). Six patients received three infusion of 10 mg (n = 3) or 40 mg (n = 3) at 2-week intervals. The pharmacokinetics were similar at both dose levels and at the second and third infusions. The plasma disappearance curves were best fit by a two-compartment model having a mean alpha T1/2 of 17.5 hr (range 13-23 hr) and a mean beta T1/2 of 100.5 hr (range 65-139 hr). One patient who received three 40-mg doses of chimeric IgG-1K 17-1A mAb (day 0, 14, and 28) was the only patient to exhibit a detectable but modest antibody reactivity to chimera on days 63 and 84. The antibody reactivity was inhibited by murine 17-1A mAb, indicating that the antibody response was directed to the murine variable region of the chimera. The patients had no toxic or allergic reactions. This chimeric form of 17-1A mAb has an approximate 6-fold longer circulation time and appears to be substantially less immunogenic than its murine counterpart. These characteristics may provide an advantage in the clinical application of such chimeric molecules in therapeutic trials in humans.
Genetic and Functional Profiling of Crohn's Disease: Autophagy Mechanism and Susceptibility to Infectious Diseases (2013)

Crohn's disease is a complex disease in which genome, microbiome, and environment interact to produce the immunological background of the disease. Disease in childhood is more extensive and characterized by a rapid progression, leading to severe repercussions in the course of the disorder. Several genetic variations have been associated with an increased risk of developing the disease and most of these are also implicated in other autoimmune disorders. The gut has many tiers of defense against incursion by luminal microbes, including the epithelial barrier and the innate and adaptive immune responses. Moreover, recent evidence shows that bacterial and viral infections, as well as inflammasome genes and genes involved in the autophagy process, are implicated in Crohn's disease pathogenesis. The aim of this review is to establish how much the diagnostic system can improve, thus increasing the success of Crohn's disease diagnosis. The major expectation for the near future is to be able to anticipate the possible consequences of the disease already in childhood, thus preventing associated complications, and to choose the best treatment for each patient.
The United Kingdom’s Role in the Community (1990)

The United Kingdom's role in the European Community is greater than would be indicated by a study of objective factors such as population, GDP per head and growth rates alone. In population size the United Kingdom is behind West Germany and Italy and only slightly ahead of France. The United Kingdom's GDP in 1987 was the sixth largest in the world behind the USA, Japan, USSR, West Germany and France but its GDP per head was eighteenth in the world ranking. In the Community, Luxembourg, Denmark, West Germany, France, the Netherlands and, arguably, Italy, had higher GDPs per head than Britain. The United Kingdom's growth rates, with rare exceptional periods, have also been lower. It is possible, if you choose your base date carefully, to argue that her growth rate in the 1980s has been the highest in Europe, but that involves a comparison from a very low starting point. Other measures such as the provision of health, welfare and social security services also put Britain fairly low in the league.
A Role for Glial Cell–Derived Neurotrophic Factor–Induced Expression by Inflammatory Cytokines and RET/GFRα1 Receptor Up-regulation in Breast Cancer (2007)

By screening a tissue microarray of invasive breast tumors, we have shown that the receptor tyrosine kinase RET (REarranged during Transfection) and its coreceptor GFR alpha 1 (GDNF receptor family alpha-1) are overexpressed in a subset of estrogen receptor-positive tumors. Germ line-activating oncogenic mutations in RET allow this receptor to signal independently of GFR alpha 1 and its ligand glial cell-derived neurotrophic factor (GDNF) to promote a spectrum of endocrine neoplasias. However, it is not known whether tumor progression can also be driven by receptor overexpression and whether expression of GDNF, as has been suggested for other neurotrophic factors, is regulated in response to the inflammatory microenvironment surrounding many epithelial cancers. Here, we show that GDNF stimulation of RET(+)/GFR alpha 1(+) MCF7 breast cancer cells in vitro enhanced cell proliferation and survival, and promoted cell scattering. Moreover, in tumor xenografts, GDNF expression was found to be up-regulated on the infiltrating endogenous fibroblasts and to a lesser extent by the tumor cells themselves. Finally, the inflammatory cytokines tumor necrosis factor-alpha and interleukin-1 beta, which are involved in tumor promotion and development, were found to act synergistically to up-regulate GDNF expression in both fibroblasts and tumor cells. These data indicate that GDNF can act as an important component of the inflammatory response in breast cancers and that its effects are mediated by both paracrine and autocrine stimulation of tumor cells via signaling through the RET and GFR alpha 1 receptors.
Cell Surface Markers of Functional Phenotypic Corneal Endothelial Cells (2014)

These markers will be useful for quality control to characterize the phenotype of cells destined for tissue engineering-based therapy. In addition, this selection protocol will provide a novel method for purification of functional cells.
Perpendicular magnetic media by anodic oxidation method and their recording characteristics (1985)

Magnetic alumite films containing electrodeposited Ni, Co and Fe were successfully prepared by employing an additional new process in the conventional anodic oxidation method in order to obtain suitable perpendicular coersive force below 1000 Oe. By this new process, the diameter of the micropores in the oxide layer was widened above 400 Å. The recording characteristics of the 5.25 inch rigid disks were evaluated using a Mn-Zn ferrite ring head with a gap length of 0.5μm. A recording density D50 of 42 kBPI was obtained for the disk with a media thickness of 1.5μm and a coercivity of 950 Oe. The output voltage was 1.2μ Vpp / μm. turn at 20 kBPI. Higher recording density of D50 was expected for the disk with a media thickness below 1.5 μm. It was shown that the present medium exhibit higher potential for recording than a conventional plated medium at high recording density region.
In Context: Responding to Family Homelessness in WA (2007)

It is understood that the risk factors for homelessness are wide ranging and complex and stem from individual circumstances and also systemic or structural factors (Wyatt, 2005); therefore it is essential that intervention responses are equally diverse and flexible.
Detailed Structure of Hairy Mixed Micelles Formed by Phosphatidylcholine and PEGylated Phospholipids in Aqueous Media (2005)

Aqueous dispersions of mixed egg yolk phosphatidylcholine (PC) and poly(ethylene glycol) (PEG) modified distearoyl phosphatidylethanolamine (DSPE) were investigated with the purpose of determining shape, size, and conformation of the formed mixed micelles. The samples were prepared at a range of DSPEPEG to PC molar ratios ([DSPEPEG/PC] from 100:0 to 30:70) and with, respectively, DSPEPEG2000 and DSPEPEG5000, where 2000 and 5000 refer to the molar masses of the PEG chains. Particle shape and internal structure were studied using small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS). The contrast of the micelles is different for X-rays and neutrons, and by combining SANS and SAXS, complementary information about the micelle structure was obtained. The detailed structure of the micelles was determined in a self-consistent way by fitting a model for the micelles to SANS and SAXS data simultaneously. In general, a model for the micelles with a hydrophobic core, surrounded by a dense hydrophilic layer that is again surrounded by a corona of PEG chains in the form of Gaussian random coils attached to the outer surface, is in good agreement with the scattering data. At high DSPEPEG contents, nearly spherical micelles are formed. As the PC content increases the micelles elongate, and at a DSPEPEG/PC ratio of 30:70, rodlike micelles longer than 1000 angstroms are formed. We demonstrate that by mixing DSPEPEG and PC a considerable latitude in controlling the particle shape is obtained. Our results indicate that the PEG chains in the corona are in a relatively unperturbed Gaussian random coil conformation even though the chains are far above the coil-coil overlap concentration and, therefore, interpenetrating. This observation in combination with the observed growth behavior questions that the "mushroom-brush"transition is the single dominating factor for determining the particle shape as assumed in previous theoretical work (Hristova, K.; Needham, D. Macromolecules 1995, 28, 991-1002).
Sensitivity and Specificity of a Rapid Whole-Blood Assay for D-Dimer in the Diagnosis of Pulmonary Embolism (1998)

A normal D-dimer test result is useful in excluding pulmonary embolism in patients with a low pretest probability of pulmonary embolism or a nondiagnostic lung scan.
Apoptosis: target of cancer therapy. (2002)

Recent knowledge on apoptosis has made it possible to devise novel approaches, which exploit this process to treat cancer. In this review, we discuss in detail approaches to induce tumor cell apoptosis, their mechanism of action, stage of development, and possible drawbacks. Finally, the obstacles yet to be overcome and the perspectives for potential clinical use of apoptosis-triggering approaches in cancer therapy in the future are assessed.
Inhibition of Rac GTPase signaling and downstream prosurvival Bcl-2 proteins as combination targeted therapy in MLL-AF9 leukemia (2011)

The Rac family of small Rho GTPases coordinates diverse cellular functions in hematopoietic cells including adhesion, migration, cytoskeleton rearrangements, gene transcription, proliferation, and survival. The integrity of Rac signaling has also been found to critically regulate cellular functions in the initiation and maintenance of hematopoietic malignancies. Using an in vivo gene targeting approach, we demonstrate that Rac2, but not Rac1, is critical to the initiation of acute myeloid leukemia in a retroviral expression model of MLL-AF9 leukemogenesis. However, loss of either Rac1 or Rac2 is sufficient to impair survival and growth of the transformed MLL-AF9 leukemia. Rac2 is known to positively regulate expression of Bcl-2 family proteins toward a prosurvival balance. We demonstrate that disruption of downstream survival signaling through antiapoptotic Bcl-2 proteins is implicated in mediating the effects of Rac2 deficiency in MLL-AF9 leukemia. Indeed, overexpression of Bcl-xL is able to rescue the effects of Rac2 deficiency and MLL-AF9 cells are exquisitely sensitive to direct inhibition of Bcl-2 family proteins by the BH3-mimetic, ABT-737. Furthermore, concurrent exposure to NSC23766, a small-molecule inhibitor of Rac activation, increases the apoptotic effect of ABT-737, indicating the Rac/Bcl-2 survival pathway may be targeted synergistically.