gebhart/patrepeval huggingface dataset:
The Electricidal Effect: Reduction of Staphylococcus and Pseudomonas Biofilms by Prolonged Exposure to Low-Intensity Electrical Current (2008)

The activity of electrical current against planktonic bacteria has previously been demonstrated. The short-term exposure of the bacteria in biofilms to electrical current in the absence of antimicrobials has been shown to have no substantial effect; however, longer-term exposure has not been studied. A previously described in vitro model was used to determine the effect of prolonged exposure (i.e., up to 7 days) to low-intensity (i.e., 20-, 200-, and 2,000-microampere) electrical direct currents on Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis biofilms. Dose- and time-dependent killing was observed. A maximum of a 6-log(10)-CFU/cm(2) reduction was observed when S. epidermidis biofilms were exposed to 2,000 microamperes for at least 2 days. A 4- to 5-log(10)-CFU/cm(2) reduction was observed when S. aureus biofilms were exposed to 2,000 microamperes for at least 2 days. Finally, a 3.5- to 5-log(10)-CFU/cm(2) reduction was observed when P. aeruginosa biofilms were exposed to electrical current for 7 days. A higher electrical current intensity correlated with greater decreases in viable bacteria at all time points studied. In conclusion, low-intensity electrical current substantially reduced the numbers of viable bacteria in staphylococcal or Pseudomonas biofilms, a phenomenon we have labeled the "electricidal effect."
Clustering of connexin 43–enhanced green fluorescent protein gap junction channels and functional coupling in living cells (2000)

Communication-incompetent cell lines were transfected with connexin (Cx) 43 fused with enhanced green fluorescent protein (EGFP) to examine the relation between Cx distribution determined by fluorescence microscopy and electrical coupling measured at single-channel resolution in living cell pairs. Cx43-EGFP channel properties were like those of wild-type Cx43 except for reduced sensitivity to transjunctional voltage. Cx43-EGFP clustered into plaques at locations of cell-cell contact. Coupling was always absent in the absence of plaques and even in the presence of small plaques. Plaques exceeding several hundred channels always conferred coupling, but only a small fraction of channels were functional. These data indicate that clustering may be a requirement for opening of gap junction channels.
Respiratory Syncytial Virus and Influenza A Infections in the Hospitalized Elderly (1995)

Respiratory syncytial virus (RSV) infections in the institutionalized elderly have been described; however, there is little information on the impact of RSV infection on community-dwelling elderly. The purpose of this study was to determine the relative numbers of hospitalizations associated with RSV infection and compare the clinical manifestations with influenza A infection. Between November and April during 1989-1992, persons > or = 65 years old hospitalized with acute cardiopulmonary conditions or influenza-like illnesses were evaluated. Evaluation included viral culture, RSV antigen detection, and serologic analysis; 159 (10%) of 1580 had RSV infection and 221 (11%) of 2091 had influenza A. RSV and influenza A cases occurred simultaneously throughout the 3 years. Clinical manifestations were similar; however, patients with RSV infection were more likely to receive therapy for bronchospasm. Death rates were 10% and 6% for RSV infection and influenza A, respectively. RSV infection is the cause of serious disease in community-dwelling older persons.
The regulation of OLFM4 expression in myeloid precursor cells relies on NF‐κB transcription factor (2008)

The human olfactomedin 4 gene (OLFM4, also known as hGC-1, GW112) is thought to be a useful marker for early myeloid development. To understand the molecular mechanisms underlying granulocyte colony-stimulating factor (G-CSF)-stimulated OLFM4 expression, we characterized the promoter region of OLFM4. The 35-bp region (-101 to -66) of the proximal promoter regulated reporter gene expression, and mutation of the nuclear factor (NF)-kappaB binding site within the promoter abolished the binding of the transcription factor and the ability to regulate OLFM4 expression. G-CSF increased reactive oxygen species (ROS) production in human CD34(+) cells, which was abrogated by inhibition of phosphatidylinositol 3-kinase (PI3K) or NADPH oxidase. Phosphorylation of ERK1/2 mitogen-activated protein kinase (MAPK) induced by G-CSF inhibited by the antioxidant N-acetyl-L-cysteine (NAC), ERK1/2 inhibitor PD98059, or U0126. However, phosphorylation of signal transducer and activator of transcription (STAT)3 was only partially inhibited by NAC, but not by PD98059 or U0126. Inhibition of the ERK pathway remarkably decreased OLFM4 expression and this inhibition required NF-kappaB transcription factor. Inhibition of ROS or the ERK pathway remarkably decreased G-CSF-induced OLFM4 expression. Our results suggest that G-CSF-induced expression of OLFM4 is regulated by the transcription factor NF-kappaB, and that this induction is mediated by the ERK1/2 MAPK signaling pathway through PI3K-driven ROS production.
Write a Classifier: Zero-Shot Learning Using Purely Textual Descriptions (2013)

The main question we address in this paper is how to use purely textual description of categories with no training images to learn visual classifiers for these categories. We propose an approach for zero-shot learning of object categories where the description of unseen categories comes in the form of typical text such as an encyclopedia entry, without the need to explicitly defined attributes. We propose and investigate two baseline formulations, based on regression and domain adaptation. Then, we propose a new constrained optimization formulation that combines a regression function and a knowledge transfer function with additional constraints to predict the classifier parameters for new classes. We applied the proposed approach on two fine-grained categorization datasets, and the results indicate successful classifier prediction.
Development and Application of an ELISA Kit for the Detection of Milk Progesterone in Dairy Cows (2014)

Progesterone (P4) is a steroid gonadal hormone that is mainly produced from the corpus luteum and placenta and has various biological functions, especially reproductive regulation. It is important to establish a specific and sensitive P4 enzyme-linked immunosorbent assay (ELISA) for the study of ovary activity and reproductive disorders. Therefore, we prepared a monoclonal antibody (MAb) in a completed antigen (11α-OH-P4-HS-OVA). Based on the MAb and our previously prepared completed antigen, a highly specific and sensitive ELISA was developed. In the present study, a competitive ELISA for the determination of P4 was described in dairy cow milk. It was found that P4 concentration in milk samples from five pregnant cows was significantly higher than that from five estrus cows. The diagnosis rate for pregnancy and non-pregnancy in 54 dairy cows were 93.3% and 95.8%, respectively, at 19 to 23 days after pregnancy by detecting milk P4 concentration. In summary, the developed ELISA is a potential tool for P4 research and offers an alternative, simple, rapid technique for detecting P4, especially in future large clinical investigations on pregnancy identification and reproductive disorders in dairy farms in China.
Preoperative Sensitivity and Specificity for Early-Stage Ovarian Cancer When Combining Cancer Antigen CA-125II, CA 15-3, CA 72-4, and Macrophage Colony-Stimulating Factor Using Mixtures of Multivariate Normal Distributions (2004)

Efficiently combining information on CA-125II, CA 72-4, and M-CSF significantly increased preoperative early-stage sensitivity from 45% with CA-125II alone to 70%, while maintaining 98% first-line specificity. Screening trials with these markers using MDA followed by referral to ultrasound may maintain previously high levels of specificity and PPV, while significantly increasing early-stage screening sensitivity. MDA is a useful, biologically justified method for combining biomarkers.
Efficacy of and tolerance to mild induced hypothermia after out-of-hospital cardiac arrest using an endovascular cooling system (2007)

The intravascular cooling system is effective, safe and allows a target temperature to be reached fairly rapidly and steadily over a period of 36 hours.
Capillary electrophoresis separation of the desamino degradation products of oxytocin (2013)

Oxytocin (OT) is an endogenous and therapeutic hormone necessary for maternal health. It is also the subject of fast growing research in the field of behavioral science. This article describes a rapid CE method using UV detection at 214 nm for the determination of the deamidation products of OT. Deamidation is the most common degradation pathway of peptides and proteins and can lead to reduced therapeutic efficiency of biopharmaceuticals. To achieve a separation of the seven structurally similar desamino peptides from OT, 11 mM sulfobutyl ether β-CD and 10% v/v MeOH were added to a BGE of 50 mM phosphate buffer at pH 6.0. The assay is linear within ≤5-100 μM for all species with a total analysis time of 12 min. The method was then applied to monitor the heat-stress degradation of OT at 70°C, where all seven desamino species were observed over a 96 h period.
Molecular cloning and gene organization of the mouse mitochondrial 3,2‐trans‐enoyl‐CoA isomerase (1993)

3,2-trans-enoyl-CoA isomerase (mECI, E.C. 5.3.3.8) is the key enzyme of mitochondrial beta-oxidation of unsaturated fatty acids. A mouse cDNA clone spanning the entire coding region of mECI was isolated and sequenced. Subsequently, two overlapping genomic clones containing the complete mECI gene were isolated and characterized. The mouse mECI cDNA comprises an open reading frame of 867 bp, encoding a protein of 32 kDa. The mECI gene, spanning about 15 kb, consists of seven exons. Multiple transcription starts were determined by primer extension experiments. Knowledge of the gene organization and availability of genomic clones for mouse mECI will facilitate the study of unsaturated fatty acid metabolism in normal and pathological states.
3DString (2006)

Classification of 3D objects remains an important task in many areas of data management such as engineering, medicine or biology. As a common preprocessing step in current approaches to classification of voxelized 3D objects, voxel representations are transformed into a feature vector description.In this article, we introduce an approach of transforming 3D objects into feature strings which represent the distribution of voxels over the voxel grid. Attractively, this feature string extraction can be performed in linear runtime with respect to the number of voxels. We define a similarity measure on these feature strings that counts common k-mers in two input strings, which is referred to as the spectrum kernel in the field of kernel methods. We prove that on our feature strings, this similarity measure can be computed in time linear to the number of different characters in these strings. This linear runtime behavior makes our kernel attractive even for large datasets that occur in many application domains. Furthermore, we explain that our similarity measure induces a metric which allows to combine it with an M-tree for handling of large volumes of data. Classification experiments on two published benchmark datasets show that our novel approach is competitive with the best state-of-the-art methods for 3D object classification.
Some approaches to best-match file searching (1973)

The problem of searching the set of keys in a file to find a key which is closest to a given query key is discussed. After “closest,” in terms of a metric on the the key space, is suitably defined, three file structures are presented together with their corresponding search algorithms, which are intended to reduce the number of comparisons required to achieve the desired result. These methods are derived using certain inequalities satisfied by metrics and by graph-theoretic concepts. Some empirical results are presented which compare the efficiency of the methods.
500-nm Optical Gain Anisotropy of Semipolar (11\bar22) InGaN Quantum Wells (2009)

We studied the effect of carrier population on light emission polarization of green InGaN quantum wells (QWs) on the semipolar (1122) plane. The 3 nm thick QWs emitting light at about 540 nm at low pumping power have electrical field (E) component E∥[1123] stronger than that E∥[1100]. However, we found that increasing the pumping power changed the sign of the polarization ratio. Using the varied stripe length (VSL) method, we measured the optical gain for light propagating ∥[1123] direction to be ∼2 times that of light propagating ∥[1100] direction. We explain this behavior by inhomogeneous QW state filling.
3D memory with shared lithography steps: The memory industry's plan to &#x201C;cram more components onto integrated circuits&#x201D; (2014)

In his 1965 paper titled “cramming more components onto integrated circuits” [1], Gordon Moore predicted the number of components on a chip would increase exponentially with time due to continual reduction of feature sizes. That paradigm has continued successfully for the past 50 years, but cracks are starting to appear. Lithography is becoming prohibitively expensive and component quality is expected to degrade significantly beyond the 7nm node. To lower cost per bit further without relying on feature size scaling, monolithic 3D flash memories are being introduced where lithography steps are shared among multiple memory layers. In this paper, I review the flash memory industry's direction and describe 3D concepts that can scale other parts of the memory hierarchy.
Comparison of two touchpad-based methods for numeric entry (2002)

Small hand-held touchpads can be used to replace stylus-based digitizing tablets when the use of a stylus is not convenient. In text entry tasks where the writing surface is held in a hand the error rate becomes a problem. The small size of strokes compared to the width of the fingertip and the additional imprecision caused by the interaction of the pad and finger movements make input very imprecise. We describe a new improved clock-face based stroke system for entering numbers with a touchpad. In a 20-session user study with 6 users we found slightly better throughput of successfully entered numbers with the proposed new system. This advantage was mainly due to lower error rate with the new system. User preference similarly slightly favored the new system over an earlier straightforward proposal based on the clock metaphor
Non-proximate detection of explosives and chemical warfare agent simulants by desorption electrospray ionization mass spectrometry (2006)

Desorption electrospray ionization (DESI) mass spectrometry is used for the selective and sensitive detection of trace amounts of explosives and chemical warfare agent simulants from ambient surfaces at distances of up to 3 meters from the mass spectrometer.
Botulinum Toxin Type A Inhibits Calcitonin Gene-Related Peptide Release From Isolated Rat Bladder (2006)

BTX-A application inhibits the evoked release of CGRP from afferent nerve terminals in isolated rat bladder. This finding suggests a potential clinical benefit of BTX-A for the treatment of interstitial cystitis or sensory urgency.
Aluminum-Germanium eutectic bonding for 3D integration (2009)

Low-temperature aluminum-germanium (Al-Ge) eutectic bonding has been investigated for monolithic three-dimensional integrated circuits (3DIC) applications. Successful bonds using Al-Ge bilayer films as thin as 157 nm were achieved at temperatures as low as 435degC, when applying 200 kPa down-pressure for 30 minutes. The liquid phase of the eutectic composition ensured a seamless and void-free bond. The fracture energy of the Al-Ge bond (630 nm thick) was measured to be G <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">c</sub> = 50.5 plusmn 12.7 J/m <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> , using double cantilever beam thin-film adhesion measurement technique. An array of silicon islands was attached onto an amorphous SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> wafer using low-temperature Al-Ge bonding. These islands could be used to form devices on upper layers of monolithically integrated 3DICs.
Design of near perfect reconstruction oversampled filter banks for subband adaptive filters (1999)

In this brief, a design algorithm for real-valued and complex-valued oversampled filter banks which yield a low level of inband alias and enable simple subband adaptive structures is presented. The filter banks are either based on complex modulation of a real-valued low-pass prototype or on the direct or modulated setups of real-valued filter banks. If real-valued filter banks are required, then the different channels will have different subsampling ratios so that the bandpass sampling theorem is not violated. This brief also presents design examples of real-valued and complex-valued filter banks.
P4–104: Hypoxia facilitates Alzheimer's disease pathogenesis (2006)

The pathogenesis of sporadic Alzheimer's disease (AD) is largely unknown. Patients with stroke or cerebral infarction showed poorer cognitive performance and greater severity of clinical dementia. Vascular risk factors have been suggested to be associated with development of AD. A reduced cerebral perfusion is a common vascular component among AD risk factors. Hypoxia is a direct consequence of hypoperfusion. The hypoxia signal transduction pathway plays a major role in vascular development and ischemia, as well as neurodegeneration. Hypoxia inducible transcription factor (HIF1) is a central mediator of hypoxia signal transduction pathway through which cells in the brain respond to reduced oxygen tension. To study if hypoxia is involved in AD pathogenesis. GSA assay was performed to identify the HRE in human and mouse BACE1 promoter. Immunohistochemical staining was used to check the plaques in the brains of mutant APP mice. The Morris water–maze test was used to test the hippocampus dependent spatial memory of APP23 mice after hypoxia treatment. Hypoxia increased APP CTF production by increasing BACE1 expression and transcription in cells. BACE1 promoter contains a functional hypoxia responsive element (HRE). BACE1 expression was significantly upregulated in mice under hypoxia treatment. Furthermore, hypoxia condition deteriorated the memory impairment in mutant APP transgenic mice. Our results suggested that hypoxia facilitated AD pathogenesis and interventions that improve cerebral perfusion might benefit AD patients.
Wavelet bicoherence: A new turbulence analysis tool (1995)

A recently introduced tool for the analysis of turbulence, wavelet bicoherence [van Milligen, Hidalgo, and Sánchez, Phys. Rev. Lett. 16, 395 (1995)], is investigated. It is capable of detecting phase coupling—nonlinear interactions of the lowest (quadratic) order—with time resolution. To demonstrate its potential, it is applied to numerical models of chaos and turbulence and to real measurements. It detected the coupling interaction between two coupled van der Pol oscillators. When applied to a model of drift wave turbulence relevant to plasma physics, it detected a highly localized coherent structure. Analyzing reflectometry measurements made in fusion plasmas, it detected temporal intermittency and a strong increase in nonlinear phase coupling coinciding with the L/H (low-to-high confinement mode) transition.
Comparative analysis of growth factors in normal and pathologic human prostates (1988)

Growth factors, as detected by DNA synthesis stimulating activity for BALB/c 3T3 cells, in normal, benign hypertrophic and cancerous human prostates were analyzed. The total content (units per gram of tissue) in benign hypertrophic prostate was two to four times higher than those in normal and cancerous prostates. In all the three groups, heparin-binding growth factor, growth factor adsorbed to heparin-Sepharose in the presence of 0.5 NaCl. accounted for about 80-95% of the total growth factor content. Qualitative analysis using a heparin-Sepharose column revealed two types of heparin-binding growth factor in the prostates, one eluted from the column at 1.2-1.3 M NaCl and the other at 1.5-1.8 M NaCl. The latter was the predominant type in all groups. In addition to the growth factors detected with BALB/c 3T3, a growth factor with specific action upon MC3T3-E1 mouse osteoblasts was found in prostatic cancer, but not in normal and benign hypertrophic prostates.
Genetic Contributions to Understanding Polyketide Synthases (1997)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTGenetic Contributions to Understanding Polyketide SynthasesDavid A. HopwoodView Author Information John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UKCite this: Chem. Rev. 1997, 97, 7, 2465–2498Publication Date (Web):November 10, 1997Publication History Received24 January 1997Revised23 April 1997Published online10 November 1997Published inissue 1 November 1997https://pubs.acs.org/doi/10.1021/cr960034ihttps://doi.org/10.1021/cr960034iresearch-articleACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views3852Altmetric-Citations558LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Bacteria,Biosynthesis,Genetics,Metabolism,Peptides and proteins Get e-Alerts