gebhart/patrepeval huggingface dataset:
Vigintiphobia, Unbound Bilirubin, and Auditory Brainstem Responses (1994)

These results suggest that measuring UBC may help in evaluating the possible risk of bilirubin encephalopathy in full-term newborns when there is vigintiphobia (fear of 20).
A New Mechanism of Action Proposed for Ramoplanin (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTA New Mechanism of Action Proposed for RamoplaninMei-Chu Lo, Hongbin Men, Arthur Branstrom, Jeremiah Helm, Nan Yao, Robert Goldman, and Suzanne WalkerView Author Information Department of Biology, Incara Pharmaceuticals Cranbury, New Jersey 08512 Department of Chemistry and Princeton Materials Institute Princeton University, Princeton, New Jersey 08544 Cite this: J. Am. Chem. Soc. 2000, 122, 14, 3540–3541Publication Date (Web):March 23, 2000Publication History Received18 January 2000Published online23 March 2000Published inissue 1 April 2000https://doi.org/10.1021/ja000182xCopyright © 2000 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views671Altmetric-Citations75LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (184 KB) Get e-AlertsSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Inhibition,Lipids,Membranes,Peptides and proteins,Polymerization Get e-Alerts
Whole genome protein domain analysis using a new method for domain clustering (1999)

Abstract We present the outcome of a systematic analysis of protein domain shuffling in 17 completed microbial genomes. This analysis has been performed using MKDOM Version 2, a completely new version of the domain clustering program MKDOM based on PSI-BLAST recursive homology searches. It allows to delineate the most frequent protein domain building blocks, which domains are found specifically in Bacteria, Archaea or yeast, and which domains are shared between two or all three domains of life. The latter are good candidates as the basic protein building blocks underlying all forms of cellular life. Statistics of multi-domain proteins indicate that some organisms such as Bacillus subtilis or Mycobacterium tuberculosis contain an abnormally high number of large multi-domain proteins. We also provide examples of highly shuffled or circularly permutated domains. A WWW graphical interface has been made available to interactively browse domain arrangements of proteins in all 17 genomes, at http://www.toulouse.inra.fr/prodomCG.html.
Purification and Characterization of TAFI, a Thrombin-activable Fibrinolysis Inhibitor (1995)

Previous studies demonstrated that tissue plasminogen activator-induced fibrinolysis in vitro is retarded in the presence of prothrombin (II) activation and that the anticoagulant-activated protein C appears profibrinolytic by preventing the formation of thrombin (IIa)-like activity during fibrinolysis. To disclose the molecular connection between the generation of IIa and the inhibition of fibrinolysis, a lysis assay that is sensitive to the antifibrinolytic effect of II activation was developed and was used to purify a 60-kDa single-chain protein from human plasma. Because the lysis of a clot, produced from purified components, is retarded when this protein is present and when II activation occurs in situ, the protein was named TAFI (thrombin-activatable fibrinolysis inhibitor). TAFI is cleaved by IIa yielding 35-, 25-, and 14-kDa products. Amino-terminal sequence analyses identified TAFI as a precursor of a plasma carboxypeptidase B (CPB). Formation of the 35-kDa product correlates with both prolongation of lysis time and CPB-like activity. Prolongation of lysis time saturates at about 125 nM TAFI. Activated TAFI inhibits the activation of Glu-plasminogen but does not prolong the lysis of clots formed in the presence of Lys-plasminogen. 2-Guanidinoethylmercaptosuccinic acid, a competitive inhibitor of CPB, completely inhibits prolongation of lysis by activated TAFI in a purified system and the prolongation induced by II activation in barium-adsorbed plasma. This suggests that TAFI accounts for the antifibrinolytic effect that accompanies prothrombin activation and that activated protein C appears profibrinolytic by attenuating TAFI activation.
Differences in the Appearance of Antibodies to Structural Components of Measles Virus after Immunization with Inactivated and Live Virus (1975)

Children immunized with inactivated (Tween 80 and diethyl ether, TE) measles vaccine, attenuated live vaccine, or a combination of the two types were studied with regard to the production of hemagglutination-inhibiting (HAI), hemolysis-inhibiting (HLI), and nucleocapsid complement-fixing (CF) antibodies. For identification of antibodies to envelope components distinct from hemagglutinin. HLI tests were done both before and after removal of HAI antibodies with TE-treated virus antigen. Immunization with three or four doses of TE vaccine led to the production of HAI antibodies and, in some cases, of nucleocapsid CF antibodies. Non-HAI HLI antibodies were not detected. In contrast, live vaccine caused the appearance of non-HAI HLI antibodies and, in the majority of cases, nucleocapsid CF antibodies. After combined immunization with TE vaccine and attenuated vaccine, non-HAI HLI antibodies were absent from 25 of 29 children. Most children exposed to wild measles virus after immunization with TE vaccine alone developed a poorer non-HAI HLI antibody response than children who had had normal measles. The latter infections resulted in a more pronounced non-HAI HLI and nucleocapsid CF antibody response than that observed after infections with vaccine virus. An even more accentuated response involving these antibodies was seen in patients with subacute sclerosing panencephalitis. Thus the failure of inactivated measles vaccines to prevent infection may be due to the absence of an envelope component responsible for the production of non-HAI HLI antibodies.
Hierarchical discriminant analysis for image retrieval (1999)

A self-organizing framework for object recognition is described. We describe a hierarchical database structure for image retrieval. The self-organizing hierarchical optimal subspace learning and inference framework (SHOSLIF) system uses the theories of optimal linear projection for optimal feature derivation and a hierarchical structure to achieve logarithmic retrieval complexity. A space-tessellation tree is generated using the most expressive features (MEF) and most discriminating features (MDF) at each level of the tree. The major characteristics of the analysis include: (1) avoiding the limitation of global linear features by deriving a recursively better-fitted set of features for each of the recursively subdivided sets of training samples; (2) generating a smaller tree whose cell boundaries separate the samples along the class boundaries better than the principal component analysis, thereby giving a better generalization capability (i.e., better recognition rate in a disjoint test); (3) accelerating the retrieval using a tree structure for data pruning, utilizing a different set of discriminant features at each level of the tree. We allow for perturbations in the size and position of objects in the images through learning. We demonstrate the technique on a large image database of widely varying real-world objects taken in natural settings, and show the applicability of the approach for variability in position, size, and 3D orientation. This paper concentrates on the hierarchical partitioning of the feature spaces.
Cell-Specific Interaction of Retinoic Acid Receptors with Target Genes in Mouse Embryonic Fibroblasts and Embryonic Stem Cells (2009)

All-trans retinoic acid (RA) induces transforming growth factor beta (TGF-beta)-dependent autocrine growth of mouse embryonic fibroblasts (MEFs). We have used chromatin immunoprecipitation to map 354 RA receptor (RAR) binding loci in MEFs, most of which were similarly occupied by the RAR alpha and RAR gamma receptors. Only a subset of the genes associated with these loci are regulated by RA, among which are several critical components of the TGF-beta pathway. We also show RAR binding to a novel series of target genes involved in cell cycle regulation, transformation, and metastasis, suggesting new pathways by which RA may regulate proliferation and cancer. Few of the RAR binding loci contained consensus direct-repeat (DR)-type elements. The majority comprised either degenerate DRs or no identifiable DRs but anomalously spaced half sites. Furthermore, we identify 462 RAR target loci in embryonic stem (ES) cells and show that their occupancy is cell type specific. Our results also show that differences in the chromatin landscape regulate the accessibility of a subset of more than 700 identified loci to RARs, thus modulating the repertoire of target genes that can be regulated and the biological effects of RA.
Assembly and secretion of heavy chains that do not associate posttranslationally with immunoglobulin heavy chain-binding protein. (1987)

Heavy chain-binding protein (BiP) associates posttranslationally with nascent Ig heavy chains in the endoplasmic reticulum (ER) and remains associated with these heavy chains until they assemble with light chains. The heavy chain-BiP complex can be precipitated by antibody reagents against either component. To identify sites on heavy chain molecules that are important for association with BiP, we have examined 30 mouse myelomas and hybridomas that synthesize Ig heavy chains with well characterized deletions. Mutant Ig heavy chains that lack the CH1 domain could not be demonstrated to associate with BiP, whereas mutant Ig heavy chains with deletions of the CH2 or CH3 domain were still able to associate with BiP. In two light chain negative cell lines that produced heavy chains with deletions of the CH1 domain, free heavy chains were secreted. When Ig assembly and secretion were examined in mutants that did not associate with BiP, and were compared with normal parental lines, it was found that the rate of Ig secretion was increased in the mutant lines and that the Ig molecules were secreted in various stages of assembly. In one mutant line (CH1-) approximately one-third of the secreted Ig molecules were incompletely assembled, whereas the Ig molecules secreted by the parental line were completely assembled. Our data show the CH1 domain to be important for association with BiP and that when this association does not occur, incompletely assembled heavy chains can be secreted. This implies a role for BiP in preventing the transport of unassembled Ig molecules from the ER.
The Heme-regulated Eukaryotic Initiation Factor 2α Kinase (2001)

Nitric oxide (NO) has been reported to inhibit protein synthesis in eukaryotic cells by increasing the phosphorylation of the alpha-subunit of eukaryotic initiation factor (eIF) 2. However, the mechanism through which this increase occurs has not been characterized. In this report, we examined the effect of the diffusible gases nitric oxide (NO) and carbon monoxide (CO) on the activation of the heme-regulated eIF2alpha kinase (HRI) in rabbit reticulocyte lysate. Spectral analysis indicated that both NO and CO bind to the N-terminal heme-binding domain of HRI. Although NO was a very potent activator of HRI, CO markedly suppressed NO-induced HRI activation. The NO-induced activation of HRI was transduced through the interaction of NO with the N-terminal heme-binding domain of HRI and not through S-nitrosylation of HRI. We postulate that the regulation of HRI activity by diffusible gases may be of wider physiological significance, as we further demonstrate that NO generators increase eIF2alpha phosphorylation levels in NT2 neuroepithelial and C2C12 myoblast cells and activate HRI immunoadsorbed from extracts of these non-erythroid cell lines.
Echocardiography of the Aortic Root (1968)

The echo pattern of the aortic root is elicited by locating the typical echo of the mitral valve and then angulating the transducer medially and sometimes cephalically. The characteristic echo pattern of the aortic root consists of paired undulating signals three to five cm apart. These signals move anteriorly during systole and posteriorly during diastole. Their position is central in relation to echoes arising from the mitral and tricuspid valves, corresponding to the anatomic position of the aortic root. The movement pattern is identical to the mitral annulus, which also represents a portion of the fibrous skeleton of the heart. Lesser echoes originating between the undulating margins of the aortic root were identified as arising from the valve cusps by correlating their motion with the production of the cardiac sounds. Further support was gained by recording abnormally intense and distorted signals in patients with calcific aortic stenosis. Anatomic validation of the aortic origin of these echoes was obtained by means of ultrasonic contrast injections made during radiologic studies of the aortic root. Saline was injected in the supravalvular position during continuous echocardiographic recording and was detected as a cloud of echoes limited by the parallel signals of the aortic root. Systolic movement of the aortic cusps was accompanied by the delivery of non-contrast blood from the left ventricle which produced defects in the contrast image paralleling the excursion of the linear signals from the cusps.
Extra-lymphatic solid tumor growth is not immunologically ignored and results in early induction of antigen-specific T-cell anergy: dominant role of cross-tolerance to tumor antigens. (2003)

A better understanding of how solid malignancies arise in an immunocompetent host, avoid immune recognition, and ultimately progress to widely disseminated cancer is essential to effectively harness the immune system against solid tumors. Because of their extra-lymphatic localization, it has been proposed that solid malignancies are just ignored by the immune system, thereby allowing their uncontrolled growth and dissemination. Alternatively, as most of the solid tumors are unable to express costimulatory molecules, the "signal one without signal two" model of tolerance induction has been frequently evoked to account for the failure of the immune system to reject antigenic tumors in vivo. In this study, we showed, however, that the extra-lymphatic growth of solid tumors is not immunologically ignored by the lymphoid compartment, resulting instead in the early induction of antigen-specific CD4(+) T-cell tolerance. Furthermore, analysis of parent-into-F1 bone marrow (BM) chimeras demonstrates that presentation of tumor antigens by BM-derived antigen-presenting cells represents the dominant mechanism in solid tumor-induced CD4(+) T-cell tolerance. Our findings of early development of antigen-specific T-cell unresponsiveness mediated by BM-derived antigen-presenting cells, not only provides a plausible explanation for the failure of the immune system to reject antigenic solid tumors in vivo, but more importantly, they have identified a barrier that, if appropriately manipulated, may lead to approaches to effectively harness the immune system against solid malignancies.
An electroacoustic recording device for wireless sensing of neural signals (2013)

Elimination of wires connecting neural recording electrodes to external electronics is highly desired, particularly in survival animal studies, due to neural damage and the device failures caused by these wires. In this study, an electroacoustic device for sensing and wireless transmission of neural signals to an external unit is proposed and results from a prototype are presented. In this method, the neural signals modulate the acoustic pulse amplitudes generated by a small piezoelectric element that is implanted at the recording site. The acoustics waves are detected wirelessly outside the nervous system by another piezoelectric transducer and the neural signals are extracted by amplitude demodulation. To test the prototype, a sinusoidal signal with 100µVpp amplitude was applied in phosphate buffered saline to simulated neural signals and the external transducer was placed 10mm away from the recording element. The results show that a sinusoidal signal of the given amplitude could be wirelessly sensed and reconstructed with a signal-to-noise ratio of 14dB.
CDDO-Me: A Novel Synthetic Triterpenoid for the Treatment of Pancreatic Cancer (2010)

Pancreatic ductal adenocarcinoma (PDA) is one of the most lethal human malignancy with dismal prognosis and few effective therapeutic options. Novel agents that are safe and effective are urgently needed. Oleanolic acid-derived synthetic triterpenoids are potent antitumorigenic agents, but their efficacy or the mechanism of action for pancreatic cancer has not been adequately investigated. In this study, we evaluated the antitumor activity and the mechanism of action of methyl-2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oate (CDDO-Me), a oleanane-derived synthetic triterpenoid for human pancreatic cancer cell lines. CDDO-Me inhibited the growth of both K-ras mutated (MiaPaca2, Panc1 and Capan2) and wild-type K-ras (BxPC3) pancreatic cancer cells at very low concentrations. The growth inhibitory activity of CDDO-Me was attributed to the induction of apoptosis characterized by increased annexin-V-FITC binding and cleavage of PARP-1 and procaspases-3, -8 and-9. In addition, CDDO-Me induced the loss of mitochondrial membrane potential and release of cytochrome C. The antitumor activity of CDDO-Me was associated with the inhibition of prosurvival p-Akt, NF-κB and mammalian target of rapamycin (mTOR) signaling proteins and the downstream targets of Akt and mTOR, such as p-Foxo3a (Akt) and p-S6K1, p-eIF-4E and p-4E-BP1 (mTOR). Silencing of Akt or mTOR with gene specific-siRNA sensitized the pancreatic cancer cells to CDDO-Me, demonstrating Akt and mTOR as molecular targets of CDDO-Me for its growth inhibitory and apoptosis-inducing activity.
Growth of human megakaryocyte colonies in culture from fetal, neonatal, and adult peripheral blood cells: ultrastructural analysis. (1979)

Megakaryocyte colonies can be grown in culture from human blood cells and fetal liver cells in plasma clot containing erythropoietin. Megakaryocyte progenitors were found in a fraction of mononuclear cells isolated by Ficoll density gradient centrifugation from adult, neonatal, and fetal blood. Megakaryocytes were identified by their morphology and particularly by their polylobulated nucleus when examined by light microscopy. The megakaryocytic nature of large cells was clearly confirmed by the presence of platelet peroxidase, demarcation membranes, and alpha-granules detected by electron microscopy; in addition mature small megakaryocytes were recognized. Megakaryocyte colonies were seen after 9 days of culture and consisted of 2 to 20 cells. The colonies were pure or mixed with the burst erythroblasts. The mixed colonies were numerous in fetal and neonatal cultures, while pure megakaryocyte colonies were seen three times more frequently in those from adult blood. The total number of colonies was also much lower in adult cultures. In colonies derived from neonatal and fetal cells, megakaryocytes often reached a more complete maturation than in those from the adults, proceeding as far as platelet shedding. This study demonstrates for the first time that a megakaryocyte committed cell present in human blood can develop megakaryocyte colonies in culture.
The Preparation and Properties of Codeinone1 (1950)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Preparation and Properties of Codeinone1Stephen P. Findlay and Lyndon F. SmallCite this: J. Am. Chem. Soc. 1950, 72, 7, 3247–3249Publication Date (Print):July 1, 1950Publication History Published online1 May 2002Published inissue 1 July 1950https://pubs.acs.org/doi/10.1021/ja01163a126https://doi.org/10.1021/ja01163a126research-articleACS PublicationsRequest reuse permissionsArticle Views173Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Antioxidants reduce cone cell death in a model of retinitis pigmentosa (2006)

Retinitis pigmentosa (RP) is a label for a group of diseases caused by a large number of mutations that result in rod photoreceptor cell death followed by gradual death of cones. The mechanism of cone cell death is uncertain. Rods are a major source of oxygen utilization in the retina and, after rods die, the level of oxygen in the outer retina is increased. In this study, we used the rd1 mouse model of RP to test the hypothesis that cones die from oxidative damage. A mixture of antioxidants was selected to try to maximize protection against oxidative damage achievable by exogenous supplements; alpha-tocopherol (200 mg/kg), ascorbic acid (250 mg/kg), Mn(III)tetrakis (4-benzoic acid) porphyrin (10 mg/kg), and alpha-lipoic acid (100 mg/kg). Mice were treated with daily injections of the mixture or each component alone between postnatal day (P)18 and P35. Between P18 and P35, there was an increase in two biomarkers of oxidative damage, carbonyl adducts measured by ELISA and immunohistochemical staining for acrolein, in the retinas of rd1 mice. The staining for acrolein in remaining cones at P35 was eliminated in antioxidant-treated rd1 mice, confirming that the treatment markedly reduced oxidative damage in cones; this was accompanied by a 2-fold increase in cone cell density and a 50% increase in medium-wavelength cone opsin mRNA. Antioxidants also caused some preservation of cone function based upon photopic electroretinograms. These data support the hypothesis that gradual cone cell death after rod cell death in RP is due to oxidative damage, and that antioxidant therapy may provide benefit.
A reconfigurable RF power amplifier biasing scheme (2004)

A reconfigurable power amplifier (PA) is studied in this paper, dedicated to multi-mode, multi-standard radio frequency front-end (RFFE), from a low cost approach. The reconfigurable amplifier topology is presented, made up of two-stages independently controllable by the biasing scheme proposed which allows the dynamic modification of the quiescent current of the RF transistor, to adapt both its linearity and its output power in order to fulfill different standards specifications.