gebhart/patrepeval huggingface dataset:
Glycosidase families (1998)

Conference Article| May 01 1998 Glycosidase families B. Henrissat B. Henrissat Centre de Recherches sur les Macromolécules Végétales1, CNRS, BP 53, F-38041, Grenoble, cedex 9, France Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd Received: November 19 1997 Online ISSN: 1470-8752 Print ISSN: 0300-5127 © 1998 Biochemical Society1998 Biochem Soc Trans (1998) 26 (2): 153–156. https://doi.org/10.1042/bst0260153 Article history Received: November 19 1997 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation B. Henrissat; Glycosidase families. Biochem Soc Trans 1 May 1998; 26 (2): 153–156. doi: https://doi.org/10.1042/bst0260153 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: HCA, hydrophobic cluster analysis This content is only available as a PDF. © 1998 Biochemical Society1998 Article PDF first page preview Close Modal You do not currently have access to this content.
Pulsing of dendritic cells with cell lysates from either B16 melanoma or MCA-106 fibrosarcoma yields equally effective vaccines against B16 tumors in mice (1998)

These results demonstrate that immunization with antigen-pulsed DC confers cellular immunity, retards tumor growth, and prolongs the survival of tumor-challenged mice. The ability of MCA-106 cell lysate-pulsed DC vaccines to inhibit the growth of subcutaneous B16 tumors also suggests the presence of shared tumor-associated antigens between these two histologically distinct tumors.
Aminopeptidase N (CD13) Is a Molecular Target of the Cholesterol Absorption Inhibitor Ezetimibe in the Enterocyte Brush Border Membrane (2004)

Intestinal cholesterol absorption is an important regulator of serum cholesterol levels. Ezetimibe is a specific inhibitor of intestinal cholesterol absorption recently introduced into medical practice; its mechanism of action, however, is still unknown. Ezetimibe neither influences the release of cholesterol from mixed micelles in the gut lumen nor the transfer of cholesterol to the enterocyte brush border membrane. With membrane-impermeable Ezetimibe analogues we could demonstrate that binding of cholesterol absorption inhibitors to the brush border membrane of small intestinal enterocytes from the gut lumen is sufficient for inhibition of cholesterol absorption. A 145-kDa integral membrane protein was identified as the molecular target for cholesterol absorption inhibitors in the enterocyte brush border membrane by photoaffinity labeling with photoreactive Ezetimibe analogues (Kramer, W., Glombik, H., Petry, S., Heuer, H., Schafer, H. L., Wendler, W., Corsiero, D., Girbig, F., and Weyland, C. (2000) FEBS Lett. 487, 293-297). The 145-kDa Ezetimibe-binding protein was purified by three different methods and sequencing revealed its identity with the membrane-bound ectoenzyme aminopeptidase N ((alanyl)aminopeptidase; EC 3.4.11.2; APN; leukemia antigen CD13). The enzymatic activity of APN was not influenced by Ezetimibe (analogues). The uptake of cholesterol delivered by mixed micelles by confluent CaCo-2 cells was partially inhibited by Ezetimibe and nonabsorbable Ezetimibe analogues. Preincubation of confluent CaCo-2 cells with Ezetimibe led to a strong decrease of fluorescent APN staining with a monoclonal antibody in the plasma membrane. Independent on its enzymatic activity, aminopeptidase N is involved in endocytotic processes like the uptake of viruses. Our findings suggest that binding of Ezetimibe to APN from the lumen of the small intestine blocks endocytosis of cholesterol-rich membrane microdomains, thereby limiting intestinal cholesterol absorption.
A combined 10/125 Mbaud twisted-pair line driver with programmable performance/power features (2002)

With shrinking supply voltages and portable local area networks (LANs) becoming more prevalent, it is becoming essential to implement transceivers dissipating minimal power. One important aspect of a transceiver is the twisted-pair driver. For 10/100 Ethernet, a typical push-pull driver can be implemented drawing 10 mA in 100Base-TX mode and 32 mA in 10Base-T mode. However, such a driver is difficult to implement using supplies lower than 3.3 V. Here, a driver is implemented in 3.3 V with the anticipation that the architecture is applicable below 3.3 V. Mechanisms allow for lower power dissipation options as a trade-off for performance.
Carboxyfluorescein succinimidyl ester‐based proliferative assays for assessment of T cell function in the diagnostic laboratory (1999)

Immune deficiency diseases are often accompanied by abnormalities in one or both arms of the specific immune system. Impairment can often be detected as a decrease in the number of T or B lymphocytes or their products in the circulation, but questions are often asked as to the functional capabilities of T lymphocytes in patients with recurrent infections. Function of T cells has traditionally been measured by their uptake of [3H]- thymidine following stimulation with antigen or mitogen in vitro. However, the ability of carboxyfluorescein succinimidyl ester (CFSE) to label lymphocytes intracellularly and track their mitotic activity by progressive two-fold reduction in fluorescence intensity prompted an alternative methodology based on flow cytometry, an approach which has the advantage of allowing specific gating on particular T cell subsets and simultaneous assessment of activation markers. This method was therefore evaluated for T cell responses to mitogen and antigen. Phytohaemagglutinin-induced blast transformation of CFSE-labelled T cells was reflected by an increase in forward and orthogonal light scatter and a progressive two-fold decrease in CFSE fluorescence intensity. These changes allowed the derivation of various measures of mitotic activity, which correlated well with [3H]-thymidine uptake. Patients with T cell functional deficiencies showed impairment in their responses by both assays, whereas the CFSE-based assay demonstrated that impaired blastogenesis was not simply due to depressed T cell numbers. Concomitant measurement of the activation markers CD69 and CD25 showed that CD69 was rapidly expressed on non-mitotic cells and that this expression was progressively diluted with subsequent rounds of cell division. In contrast, CD25 expression was unaffected by cell cycle, but was expressed in proportion to the PHA dose. Antigen-specific responsiveness to Candida was also assessed using a CFSE-based assay. Initial gating on the relatively minor population of T cells that underwent blast transformation demonstrated progressive twofold dilutions of CFSE intensity in responsive cells. These normal Candida responses, found in patients who had recovered from Candida infection, contrasted with those who had not been infected with Candida or who had chronic recurrent infection, in whom neither blast transformation nor significant mitosis could be detected. Again, there was good correlation with [3H]-thymidine uptake. The CFSE-based assays are equivalent to traditional measures of mitogen- and antigen-specific T cell responsiveness in the diagnostic laboratory and have significant advantages in terms of decreased labour intensiveness, avoidance of radioactivity, the ability to gate on a specific population of lymphocytes and the concomitant measurement of activation markers.
Light Trapping in Thin-Film Silicon Solar Cells for Superstrate and Substrate Configurations (2010)

Abstract not Available.
Radiation Effects on Polymers (1991)

Recent or picosecond (PS) and nanosecond (NS) electron beam (EB), ion beam (IB), and synchrotron radiation (SR) pulse radiolysis studies on polymers are reviewed. Transient absorption and emission spectra of short-lived reactive intermediates such as electrons, radical anions and cations, excited states and neutral radicals have been observed in irradiated solid polymers and polymers solutions, such as polyethylene model compounds and ethylene-propylene copolymers, polystyrene and related compounds including resists, and sigma-conjugated polymers such as polysilanes and polygermanes. Very recent progress in our picosecond EB pulse radiolysis systems, especially new LL (linac-laser) twin system, is also shown. IB and SR pulse radiolysis systems (1 ns and subnanosecond time resolution for emission spectroscopy, respectively) are developed very recently and applied to polymers. The history of pulse radiolysis studies on polymers is also reviewed shortly.
Effect of carbon doping on buffer leakage in AlGaN/GaN high electron mobility transistors (2004)

Carbon doping via CBr4 in AlGaN/GaN high electron mobility transistors grown by rf-plasma-assisted molecular beam epitaxy on 4H–SiC (0001) was investigated as a means to reduce buffer leakage. For carbon doping in the first 400 nm of the structure, a significant decrease in buffer leakage was observed with increasing overall carbon concentration. A carbon doping scheme in which the level of doping is tapered from 6×1017 cm−3 down to 2×1017 cm−3 was found to result in sufficiently low drain-source leakage currents. The effect of thickness of the GaN:C layer was explored as well as the effect of thickness of the subsequent unintentionally doped GaN layer. For structures with reduced leakage, rf I–V and power measurements revealed better performance in structures in which the two-dimensional electron gas was spaced at a large distance from the GaN:C layer. Possible sources and locations of unintentional free carriers contributing to leakage in these structures are discussed in light of the results.
The role of calcium ions in the closing of K channels. (1986)

The effects of external Ca ion on K channel properties were studied in squid giant axons. Increasing the Ca concentration from 20 to 100 mM slowed K channel opening, and was kinetically equivalent to decreasing the depolarizing step by approximately 25 mV. The same Ca increase had a much smaller effect on closing kinetics, equivalent to making the membrane potential more negative by approximately mV. With regard to the conductance-voltage curve, this Ca increase was about equivalent to decreasing the depolarizing step by approximately 10 mV. The presence of K or Rb in the bath slowed closing kinetics and made the time course more complex: there were pronounced slow components in Rb and, to a lesser extent, in K. Increasing the Ca concentration strongly antagonized the slowing caused by Rb or K. Thus, Ca has a strong effect on closing kinetics only in the presence of these monovalent cations. Rb and K do not significantly alter opening kinetics, nor do they alter Ca's ability to slow opening kinetics. High Ca slightly affects the instantaneous I-V curve by selectively depressing inward current at negative voltages. The results imply that Ca has two actions on K channels, and in only one, the action on closing, does it compete with monovalent cations. We propose (a) that opening kinetics are slowed by binding of Ca to negatively charged parts of the gating apparatus that are at the external surface of the channel protein when the channel is closed; monovalent cations do not compete effectively in this action; (b) Ca (or possibly Mg) normally occupies closed channels and has a latching effect. External K or Rb competes with Ca for channel occupancy. Channels close sluggishly when occupied by a monovalent cation and tend to reopen. Thus, slow closing results from occupancy by K or Rb instead of Ca. The data are well fit by a model based on these ideas.
Influence of chronic captopril therapy on the infarcted left ventricle of the rat. (1985)

To determine whether the relationship between infarct size and ventricular performance, volume, and compliance could be altered favorably, captopril was administered to rats for 3 months following coronary artery ligation. Baseline left and right ventricular and systemic arterial pressures and aortic blood flow, and maximal stroke volume and cardiac indices attained during a volume loading, were measured. Passive pressure-volume relations of the left ventricle were determined, and the slopes of segments of this relation were analyzed to characterize ventricular chamber stiffness. In untreated rats, left ventricular end-diastolic pressure progressively rose (from 5-28 mm Hg) as a function of infarct size, whereas, in captopril-treated rats, filling pressure remained within normal limits (5 +/- 1 mm Hg) in all but those with extensive infarcts. Chronic captopril therapy reduced baseline mean arterial pressure and total peripheral resistance, yet maintained cardiac and stroke outputs in rats both with and without infarcts. In untreated rats, maximal pumping ability progressively declined with increasing infarct size, whereas, in captopril-treated rats, peak stroke volume index remained within normal limits in all but those with extensive infarcts. The in vitro left ventricular volumes of captopril-treated rats were significantly less than those of untreated rats. The maintenance of forward output from a lesser dilated left ventricle yielded an index of ejection fraction for treated rats with moderate and large infarcts that was significantly elevated compared with that of untreated rats with infarcts of comparable size. Left ventricular chamber stiffness, which fell as infarct size increased in untreated rats, was normalized by chronic captopril therapy. Thus, captopril attenuated the left ventricular remodeling (dilation) and deterioration in performance that were observed in rats with chronic myocardial infarction.
Detection of Viable Cryptosporidium parvum Using DNA-Modified Liposomes in a Microfluidic Chip (2001)

This paper describes a microfluidic chip that enables the detection of viable Cryptosporidium parvum by detecting RNA amplified by nucleic-acid-sequence-based amplification (NASBA). The mRNA serving as the template for NASBA is produced by viable C. parvum as a response to heat shock. The chip utilizes sandwich hybridization by hybridizing the NASBA-generated amplicon between capture probes and reporter probes in a microfluidic channel. The reporter probes are tagged with carboxyfluorescein-filled liposomes. These liposomes, which generate fluorescence intensities not obtainable from single fluorophores, allow the detection of very low concentrations of targets. The limit of detection of the chip is 5 fmol of amplicon in 12.5 microL of sample solution. Samples of C. parvum that underwent heat shock, extraction, and amplification by NASBA were successfully detected and clearly distinguishable from controls. This was accomplished without having to separate the amplified RNA from the NASBA mixture. The microfluidic chip can easily be modified to detect other pathogens. We envision its use in mu-total analysis systems (mu-TAS) and in DNA-array chips utilized for environmental monitoring of pathogens.
Curvature maps for local shape comparison (2006)

The ability to identify similarities between shapes is important for applications such as medical diagnosis, object registration and alignment, and shape retrieval. In this paper we present a method, the curvature map that uses surface curvature properties in a region around a point to create a unique signature for that point. These signatures can then be compared to determine the similarity of one point to another. To gather curvature information around a point we explore two techniques, rings (which use the local topology of the mesh) and geodesic fans (which trace geodesies along the mesh from the point). We explore a variety of comparison functions and provide experimental evidence for which ones provide the best discriminatory power. We show that curvature maps are both more robust and provide better discrimination than simply comparing the curvature at individual points.
Epidemiological survey of the orthopaedic status of severe haemophilia A and B patients in France (2000)

One hundred and 16 patients contributed to an analysis of the impact of the consequences of severe haemophilia A or B (factor levels < 2%) on orthopaedic status, resources consumed in relation to this status and resultant cost, and quality of life as perceived by the patient, using the MOS 36-Item-Short-Form Health Survey (SF-36). This French cross-sectional study involved outpatients regularly attending a haemophilia treatment centre. Data were collected retrospectively over a period of 1 year by the physician of the haemophilia treatment centre. Patients had a mean age of 23, and consisted of 50% students, 25% salaried workers, 17.2% with no professional activity and 7.8% physically impaired; 82.8% of them had type A haemophilia. Mean pain score was 2.5 per patient for the six main joints; 7.7 for the clinical score and 18.8 for the radiological score, with a mean number of bleeds of 16.3 per year per patient. During the year prior to inclusion, and because of their orthopaedic status, 22.4% of patients were hospitalized, 76.7% attended for an outpatient visit and 76.7% required at least one special investigation; 97.4% received replacement therapy, 41.4% required treatment for joint pain and 42.2% orthopaedic equipment. The less affected dimensions were the physical function (76.8 +/- 22. 2) and the social relations (76.1 +/- 23.1). Least good quality of life scores concerned the pain (60.2 +/- 25.2), perception of general health (59.3 +/- 23.1) and vitality (57.8 +/- 19.5) dimensions. The age was a discriminant criterion since quality of life was better in patients of the 18-23 age group for five dimensions. Mean annual treatment costs of a patient with severe haemophilia were determined as 425 762 French francs ($73 029). Loss of production was estimated at a mean of 4609 French francs ($791) per active patient over the course of the year. Results showed indirect evidence of the usefulness of early home treatment.
Sterols. IX. Isolation of epi-Pregnanol-3-one-20 from Human Pregnancy Urine (1937)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSterols. IX. Isolation of epi-Pregnanol-3-one-20 from Human Pregnancy UrineRussell E. Marker, Oliver Kamm, and Ralph V. McGrewCite this: J. Am. Chem. Soc. 1937, 59, 4, 616–618Publication Date (Print):April 1, 1937Publication History Published online1 May 2002Published inissue 1 April 1937https://pubs.acs.org/doi/10.1021/ja01283a006https://doi.org/10.1021/ja01283a006research-articleACS PublicationsRequest reuse permissionsArticle Views54Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Superovulation Using CIDR in Holstein Cows. (1994)

Three trials were conducted to investigate the superovulatory response of Control Internal Drug Releasing device (CIDR(R)). Forty-six Holstein cows were divided into three treatment groups per trial; CIDR(R) without progesterone (P4) (Control), CIDR(R) containing 1.9 g P4 for 12 days (CIDR(R)-1) and the two CIDRs(R) with P4 replacing with a new CIDR(R) on day 9 of the 12 day insertion period (CIDR(R)-2). Follicle stimulating hormone (FSH) were administered on day 9 up to the time of CIDR(R) removal and additional prostaglandin F2 injections were done on day 11 of the 12 day insertion period. Ovulation rate, recovered and transferable embryos, and P, and estradiol-17 (E2) levels in blood plasma were evaluated and compared among the treatment groups. There were no statistical differences on the mean (} SEM) ovulation rate, recovered and transfer-
<title>Efficient accurate liquid crystal digital light deflector</title> (1999)

The Digital Light Deflector (DLD) can be used to deflect a laser beam without using moving parts. The DLD consists of cascaded pairs of deflecting and switching elements. In each stage, a birefringent element deflects the incident beam by two possible angles. The accompanying electro-optic switch selects the incident linear polarization state, thus deciding the output deflection for that stage. By cascading together N stages with successively increasing deflection angles, 2-to-the-power-N output angles are possible. Many earlier DLD devices utilized inorganic electro-optic materials and required high power. The only prior low-power DLD, using nematic liquid crystal deflectors, suffered from significant scattering losses and was not optimized for wide-angle steering. We present an improved liquid crystal DLD (LC-DLD). This LC-DLD makes use of smectic A liquid crystalline deflecting prisms, resulting in a substantial reduction of scattering losses. This device can also be designed to reduce steering error at wide angles. Steering error reduction is accomplished by optimizing the geometry and orientation of each deflector. Fabrication methods, guidelines for choice of viable smectic materials, and design optimization techniques are discussed.
Synthesis and characterization of 1- and 2-monoglycerides of anteiso fatty acids (1966)

The branched-chain fatty acids D-(+)-12-methyltetradecanoic acid (C(15) anteiso) and D-(+)-14-methylhexadecanoic acid (C(17) anteiso) were isolated from the lipids of Listeria monocytogenes and their 1- and 2-monoglycerides were prepared. Reaction intermediates and products were purified without isomerization by column chromatography. Thin-layer chromatography on Florisil impregnated with boric acid and nuclear magnetic resonance were used in characterizing the 1- and 2-monoglycerides. The value of the latter method for analyzing glyceride structure is discussed.
Effective Treatment of Advanced Multiple Myeloma Refractory to Alkylating Agents (1984)

Twenty-nine patients with advanced refractory multiple myeloma were treated with intermittent high-dose dexamethasone in combination with four-day infusions of vincristine and doxorubicin. Rapid and marked tumor-mass reduction (greater than 75 per cent) was noted in 14 of 20 patients whose disease was resistant to alkylating agents and in 3 of 9 patients with additional resistance to doxorubicin--a result far superior to those in previous trials with similar patients. In responsive patients, remissions were of excellent quality, and survival was significantly longer than in unresponsive patients.