gebhart/patrepeval huggingface dataset:
Human glial fibrillary acidic protein: complementary DNA cloning, chromosome localization, and messenger RNA expression in human glioma cell lines of various phenotypes. (1991)

Glial fibrillary acidic protein (GFAP) is a constituent of intermediate filaments of glial cells of the astrocyte lineage. We cloned a human GFAP complementary DNA, deduced the amino acid sequence, and established the chromosomal location (17q21) of the GFAP gene by Southern blot hybridization of somatic cell hybrids and by in situ hybridization. The authenticity of the complementary DNA was proven by expressing it in glioma cells lacking endogenous GFAP; after microinjection of the complementary DNA, such cells became positive for staining with GFAP antibodies. The levels of fibronectin (FN) and GFAP mRNA of ten human glioblastoma cell lines, determined by Northern blot hybridization of RNA, were related to other phenotypic characteristics [cell morphology and expression of the genes encoding platelet-derived growth factor (PDGF) receptors]. A high expression of GFAP mRNA was found only in cells lacking fibronectin mRNA and protein. Glioma cells with a fibroblastic phenotype (bipolar, FN+/GFAP-) were found to express both types of PDGF receptors (alpha and beta). Relatively high levels of PDGF alpha-receptor mRNA, in the absence of beta-receptor expression, were found in cell lines that express GFAP and lack detectable levels of fibronectin mRNA. The findings are compatible with the idea that the genes encoding PDGF receptors in glioma cells are regulated in concert with other genes, the expression of which may reflect the developmental program of normal glia cell lineages.
Human membrane type‐4 matrix metalloproteinase (MT4‐MMP) is encoded by a novel major transcript: isolation of complementary DNA clones for human and mouse mt4‐mmp transcripts (1999)

Five distinct membrane-type matrix metalloproteinases (MT-MMP) have been reported by cDNA cloning. However, the mt4-mmp gene product (MMP-17) has not been identified yet in spite of the cDNA isolation [Puente et al. (1996), Cancer Res. 56, 944-949]. In this study, we re-examined the transcripts for human mt4-mmp by 5' RACE and identified two types of transcripts. The minor one corresponded to the cDNA reported by Puente et al. and failed to express protein, and the other is the major transcript that has an extended open reading frame and expressed 67 and 71 kDa translation products. Thus, functional mt4-mmp has been identified for the first time.
The Preventive Treatment of Migraine (2006)

Preventive therapies are well suitable for migraineurs with attack-related disability despite appropriate acute care.
Transform-domain Wyner-Ziv codec for video (2004)

In current interframe video compression systems, the encoder performs predictive coding to exploit the similarities of successive frames. The Wyner-Ziv Theorem on source coding with side information available only at the decoder suggests that an asymmetric video codec, where individual frames are encoded separately, but decoded conditionally (given temporally adjacent frames) could achieve similar efficiency. We propose a transformdomain Wyner-Ziv coding scheme for motion video that uses intraframe encoding, but interframe decoding. In this system, the transform coefficients of a Wyner-Ziv frame are encoded independently using a scalar quantizer and turbo coder. The decoder uses previously reconstructed frames to generate side information to conditionally decode the Wyner-Ziv frames. Simulation results show significant gains above DCT-based intraframe coding and improvements over the pixel-domain Wyner-Ziv video coder.
Evaluation of off‐target effects of gapmer antisense oligonucleotides using human cells (2019)

Antisense oligonucleotide (ASO) has the potential to induce off-target effects due to complementary binding between the ASO and unintended RNA with a sequence similar to the target RNA. Conventional animal studies cannot be used to assess toxicity induced by off-target effects because of differences in the genome sequence between humans and other animals. Consequently, the assessment of off-target effects with in silico analysis using a human RNA database and/or in vitro expression analysis using human cells has been proposed. Our previous study showed that the number of complementary regions of ASOs with mismatches in the human RNA sequences increases dramatically as the number of tolerated mismatches increases. However, to what extent the expression of genes with mismatches is affected by off-target effects at the cellular level is not clear. In this study, we evaluated off-target effects of gapmer ASOs, which cleave the target RNA in an RNase H-dependent manner, by introducing the ASO into human cells and performing microarray analysis. Our data indicate that gapmer ASOs induce off-target effects depending on the degree of complementarity between the ASO and off-target candidate genes. Based on our results, we also propose a scheme for the assessment of off-target effects of gapmer ASOs.
Induction of remission by interferon-alpha in patients with chronic active ulcerative colitis. (1995)

In our opinion, IFN-alpha 2a is a well-tolerated, non-toxic, easily applicable and outstanding drug, which could become the treatment of choice for first-line therapy of chronic active ulcerative colitis, especially in patients who show resistance to other drugs.
Structure-Function Analysis of the Prosegment of the Proprotein Convertase PC5A (2003)

To investigate if some residues within the prosegment of PC5A are important for its optimal proteolytic function, various PC5A mutants were cellularly expressed, and their processing activities were compared using pro-vascular endothelial growth factor C (pro-VEGF-C) as a substrate. Although wild type PC5A almost completely processes pro-VEGF-C, a prosegment deletion as well as both P1 mutants of the primary (R116A) and secondary (R84A) autocatalytic cleavage sites are inactive. The in vitro inhibitory potency of various decapeptides mimicking the C-terminal sequence of PC5 prosegment (pPC5) revealed that the native (107)QQVVKKRTKR(116) peptide is a nanomolar inhibitor, whereas its P6 mutant K111H is more selective toward PC5A than Furin. In vitro activity assays using the bacterially expressed pPC5 and its mutants revealed them to be very potent nanomolar inhibitors (IC(50)) and only approximately 6-fold more selective inhibitors of PC5A versus Furin. Expression of the preprosegment of PC5 (ppPC5) and its mutants in Chinese hamster ovary FD11 cells overexpressing pro-VEGF-C with either PC5A or Furin showed them to be as good inhibitors of PC5A as the serpin alpha1-antitrypsin Portland (alpha1-PDX), ppFurin, or ppPACE4 but less potent toward overexpressed Furin. In conclusion, cleavages of the prosegment of PC5A at both Arg(116) and Arg(84) are required for PC5A cellular activity, and ppPC5 is a very potent but modestly selective cellular inhibitor of PC5A.
Molecular Basis of the Interaction between Proapoptotic Truncated BID (tBID) Protein and Mitochondrial Carrier Homologue 2 (MTCH2) Protein (2012)

The molecular basis of the interaction between mitochondrial carrier homologue 2 (MTCH2) and truncated BID (tBID) was characterized. These proteins participate in the apoptotic pathway, and the interaction between them may serve as a target for anticancer lead compounds. In response to apoptotic signals, MTCH2 recruits tBID to the mitochondria, where it activates apoptosis. A combination of peptide arrays screening with biochemical and biophysical techniques was used to characterize the mechanism of the interaction between tBID and MTCH2 at the structural and molecular levels. The regions that mediate the interaction between the proteins were identified. The two specific binding sites between the proteins were determined to be tBID residues 59-73 that bind MTCH2 residues 140-161, and tBID residues 111-125 that bind MTCH2 residues 240-290. Peptides derived from tBID residues 111-125 and 59-73 induced cell death in osteosarcoma cells. These peptides may serve as lead compounds for anticancer drugs that act by targeting the tBID-MTCH2 interaction.
Liquid Fragility and the Glass Transition in Water and Aqueous Solutions (2002)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLiquid Fragility and the Glass Transition in Water and Aqueous SolutionsC. A. AngellView Author Information Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604 Cite this: Chem. Rev. 2002, 102, 8, 2627–2650Publication Date (Web):July 30, 2002Publication History Received18 September 2001Published online30 July 2002Published inissue 1 August 2002https://pubs.acs.org/doi/10.1021/cr000689qhttps://doi.org/10.1021/cr000689qresearch-articleACS PublicationsCopyright © 2002 American Chemical SocietyRequest reuse permissionsArticle Views5412Altmetric-Citations526LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Amorphous materials,Glass transition,Liquids,Solution chemistry,Viscosity Get e-Alerts
Kempe,Margery (2017)

Margery Kempe (c. 1373–after 1438) was a middle‐class, married laywoman and mother who had numerous visions of God and Christ. Her visionary encounters are documented in the Middle English Book of Margery Kempe , which she dictated in collaboration with a priest. Her spiritual life is characterized by affective mysticism, visions, and public weeping; her unconventional life led to numerous controversial encounters with male clerical authorities. In her life, she was accused of Lollardy and hypocrisy, particularly with regard to her sexuality and desire to live chastely with her husband. The Book is also notable for its use of hagiographic conventions, although Kempe herself was never canonized.
Aggregation of IgG Globulin in Vivo. II. Physicochemical Properties of the Isolated Protein* (1966)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAggregation of IgG Globulin in Vivo. II. Physicochemical Properties of the Isolated Protein*Shaul Kochwa, Eugene Smith, Marilyn Brownell, and Louis R. WassermanCite this: Biochemistry 1966, 5, 1, 277–285Publication Date (Print):January 1, 1966Publication History Published online1 May 2002Published inissue 1 January 1966https://pubs.acs.org/doi/10.1021/bi00865a036https://doi.org/10.1021/bi00865a036research-articleACS PublicationsRequest reuse permissionsArticle Views80Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Ripple Effect: Emotional Contagion and its Influence on Group Behavior (2002)

Group emotional contagion, the transfer of moods among people in a group, and its influence on work group dynamics was examined in a laboratory study of managerial decision making using multiple, convergent measures of mood, individual attitudes, behavior, and group-level dynamics. Using a 2 times 2 experimental design, with a trained confederate enacting mood conditions, the predicted effect of emotional contagion was found among group members, using both outside coders' ratings of participants' mood and participants' self-reported mood. No hypothesized differences in contagion effects due to the degree of pleasantness of the mood expressed and the energy level with which it was conveyed were found. There was a significant influence of emotional contagion on individual-level attitudes and group processes. As predicted, the positive emotional contagion group members experienced improved cooperation, decreased conflict, and increased perceived task performance. Theoretical implications and practical ramifications of emotional contagion in groups and organizations are discussed.
Clinical Experience with Expanded Polytetrafluoroethylene Gore-Tex® Surgical Membrane for Pericardial Closure: A Study of 110 Cases (1988)

Complete closure of the pericardium after cardiac operations has the advantage of avoiding injury of the heart and great vessels during reoperation. Between 1985 and 1987, the pericardium was closed with Gore-Tex Surgical Membrane (SM) in a selected series of 110 patients 1 month to 76 years of age. Fifty-three patients had congenital heart lesions and 57 patients had acquired heart disease. Overall hospital mortality was 3/110 cases. In no instance was there a relationship between occurrence of death and pericardial closure with SM. There was one episode of cardiac tamponade on the seventh postoperative day. One patient developed fever and leukocytosis due to a mediastinal hematoma. During a mean follow-up of 15 months, four patients had to be reoperated upon three, four, eight weeks, and eight months after primary operation. The anterior wall of the heart had no adhesion with the SM and the other parts of pericardium could be dissected easily. Scanning electron microscopic examination of the explanted SM patches showed neither cellular ingrowth nor immunocompetent cellular elements. The Gore-Tex Surgical Membrane has the advantages of easy availability and lack of reaction between its surface and the epicardium and pericardium. We believe its routine use should be encouraged in patients with high probability of reoperation after repair of complex cardiac anomalies, implantation of bioprostheses, coronary revascularization for one- or two-vessel disease, and repair of degenerative disease of the ascending aorta.
대구경 현장타설말뚝에 대한 변형봉 센서의 현장적용성에 관한 연구 (2003)

강관 내부 속채움한 현장타설말뚝에 대한 기존의 하중전이 측정에서는 강관의 변형률만 측정하고 콘크리트의 변형률은 강관과 동일하다고 가정하였으며, 시방서에 규정한 방법으로 구한 강관과 콘크리트의 탄성계수를 이용하여 말뚝구성부재의 응력 및 축하중을 산정하였다. 그러나 강관의 변형률만 측정하여 강관과 콘크리트가 완전차성 거동하는 것으로 산정한 축하중은 실제 하중값과 상당한 차이를 보이고 있어 강관 내부 속채움한 현장타설말뚝의 거동을 정확히 분석할 수 없었다. 본 연구에서는 현장에서 제작한 콘크리트 체의 압축강도 시험을 통하여 탄성계수를 구하고 강재와 콘크리트의 변형률을 각각 측정할 수 있는 변형봉 센서를 이용하여 새로운 말뚝축하중 측정 방법을 제안하였다. 변형봉 센서를 사용하여 말뚝축하중을 산정할 경우 콘크리트의 탄성계수는 현장에서 제작한 콘크리트 공시체의 압축강도 시험에서 구하였으며, (0.2 ~O.6)f_(ck)의 응력 범위에 해당하는 평균접선기운기를 탄성계수로 사용하였다. 세개의 현장타설말뚝에 대해 수행된 하중전이 측정 실험 결과를 이용하여 현장 적용성을 확인하였다. 변형봉 센서의 적용성은 대구경 현장타설말뚝에 대한 축하중 분포도를 통하여 확인하였는데, 말뚝머리에서 계산된 하중은 강관 내부 속채움한 현장타설말뚝의 경우 실제작용하중에 비하여 -11 ~-16% 오차를 나타내었으며, 현장타설 철근콘크리트말뚝의 경우 3.4% 오차를 나타내었다.
Protein Tyrosine Phosphatases: Mechanism of Catalysis and Substrate Specificity (1994)

This chapter contains sections titled: Introduction Mechanism of Catalysis Substrate Specificity Conclusion and Perspective
Chemistry of oxaziridines. 13. Synthesis, reactions, and properties of 3-substituted 1,2-benzisothiazole 1,1-dioxide oxides (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemistry of oxaziridines. 13. Synthesis, reactions, and properties of 3-substituted 1,2-benzisothiazole 1,1-dioxide oxidesFranklin A. Davis, James C. Towson, Dhiru B. Vashi, R. ThimmaReddy, John P. McCauley Jr., Mark E. Harakal, and Donald J. GosciniakCite this: J. Org. Chem. 1990, 55, 4, 1254–1261Publication Date (Print):February 1, 1990Publication History Published online1 May 2002Published inissue 1 February 1990https://pubs.acs.org/doi/10.1021/jo00291a028https://doi.org/10.1021/jo00291a028research-articleACS PublicationsRequest reuse permissionsArticle Views2550Altmetric-Citations74LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Generation of 98 fs optical pulses directly from an erbium-doped fibre ring laser at 1.57 μm (1993)

Optical pulses as short as 98 fs at 1.57 μm have been successfully generated for the first time directly from a laserdiode pumped erbium-doped fibre laser with a nonlinear amplifying loop mirror. By using a low GVD polarisation sensitive isolator and reducing the fibre length in a nonlinear loop, 124 fs pulses were obtained. They were further shortened to 98 fs by installing an EDFA as an active pulse compressor and a positive GVD fibre for chirp compensation.
Mitochondrial Gene Therapy Augments Mitochondrial Physiology in a Parkinson's Disease Cell Model (2009)

Neurodegeneration in Parkinson's disease (PD) affects mainly dopaminergic neurons in the substantia nigra, where age-related, increasing percentages of cells lose detectable respiratory activity associated with depletion of intact mitochondrial DNA (mtDNA). Replenishment of mtDNA might improve neuronal bioenergetic function and prevent further cell death. We developed a technology ("ProtoFection") that uses recombinant human mitochondrial transcription factor A (TFAM) engineered with an N-terminal protein transduction domain (PTD) followed by the SOD2 mitochondrial localization signal (MLS) to deliver mtDNA cargo to the mitochondria of living cells. MTD-TFAM (MTD = PTD + MLS = "mitochondrial transduction domain") binds mtDNA and rapidly transports it across plasma membranes to mitochondria. For therapeutic proof-of-principle we tested ProtoFection technology in Parkinson's disease cybrid cells, using mtDNA generated from commercially available human genomic DNA (gDNA; Roche). Nine to 11 weeks after single exposures to MTD-TFAM + mtDNA complex, PD cybrid cells with impaired respiration and reduced mtDNA genes increased their mtDNA gene copy numbers up to 24-fold, mtDNA-derived RNAs up to 35-fold, TFAM and ETC proteins, cell respiration, and mitochondrial movement velocities. Cybrid cells with no or minimal basal mitochondrial impairments showed reduced or no responses to treatment, suggesting the possibility of therapeutic selectivity. Exposure of PD but not control cybrid cells to MTD-TFAM protein alone or MTD-TFAM + mtDNA complex increased expression of PGC-1alpha, suggesting activation of mitochondrial biogenesis. ProtoFection technology for mitochondrial gene therapy holds promise for improving bioenergetic function in impaired PD neurons and needs additional development to define its pharmacodynamics and delineate its molecular mechanisms. It also is unclear whether single-donor gDNA for generating mtDNA would be a preferred therapeutic compared with the pooled gDNA used in this study.
Critical Flanking Sequences of PU.1 Binding Sites in Myeloid-specific Promoters (1999)

The myeloid-specific transcription factor PU.1 is essential for expression of p47(phox), a component of the superoxide-forming phagocyte NADPH oxidase. The consensus PU.1 binding sequence (GAGGAA) is located on the non-coding strand from position -40 to -45 relative to the transcriptional start site of the p47phox promoter. A promoter construct extending to -46 was sufficient to drive tissue-specific expression of the luciferase reporter gene, but extension of the promoter from -46 to -48 resulted in a significant increase in reporter expression. Mutations of the nucleotides G at -46 and/or T at -47 reduced both reporter expression and PU.1 binding, whereas mutations at -48 had no effect. The PU.1 binding avidity of these sequences correlated closely with their capacity to dictate reporter gene transcription. In parallel studies on the functional PU.1 site in the promoter of CD18, mutations of nucleotides G and T at positions -76 and -77 (corresponding to -46 and -47, respectively, of the p47phox promoter) reduced PU.1 binding and nearly abolished the contribution of this element to promoter activity. We conclude that the immediate flanking nucleotides of the PU.1 consensus motif have significant effects on PU.1 binding avidity and activity and that this region is the dominant cis element regulating p47phox expression.
Effects of nocturnal gamma-hydroxybutyrate on sleep/waking patterns in narcolepsy-cataplexy. (1980)

Continuous 48-hour polygraphic recordings of sleep/waking patterns were performed on 14 patients with narcolepsy-cataplexy before and after 7-10 days of treatment of their nocturnal sleep with gamma-hydroxybutyrate (GBH). GBH improved the quality of night sleep by increasing the amount of slow wave sleep, reducing stage I, increasing sleep efficiency (percentage of time in bed spent asleep), and reducing the number of periods of short sleep under 15 minutes. Also nighttime REM sleep was reduced in latency and became less fragmented. The daytime period contained less slow wave sleep and REM sleep, and fewer episodes of prolonged sleep. Patients experienced reduction or loss of daytime attacks of irresistible sleep, cataplectic attacks, and other auxiliary symptoms. Residual daytime drowsiness subsequently improved on low doses of methylphenidate. Tolerance did not develop and there were no serious toxic side-effects. Four of the patients had been refractory to previous combinations of antidepressants and high doses of stimulants.
[Normalizing effect of intermittent hypoxic training on the function of endothelium in experimental diabetes mellitus]. (2007)

The purpose of the study was to investigate changes of endothelial function at experimental diabetes mellitus under the action of interval hypoxic trainings (IHT). A decrease of hyperglycemia and normalization of endothelium-dependent vascular reactions are shown after IHT. Activity of constitutive NOS in the heart after IHT increases considerably while activity of inducible NOS decreases sharply. Considerable diminution of iNOS activity after IHT also registered in aorta. Nitrite-anion content (NO2-) increased both in the heart and in aorta. IHT results in diminishing level of such markers of oxidizing stress as a hydrogen peroxide and dien conjugates. Thus, the action of IHT consist in diminishing ofhyperglycemia, decline of expressed of oxidizing stress, partial normalization of synthesis of nitric oxide and renewal of endothelial function in rats with experimental diabetes.