gebhart/patrepeval huggingface dataset:
Control of Lactose Transport, β-Galactosidase Activity, and Glycolysis by CcpA in Streptococcus thermophilus : Evidence for Carbon Catabolite Repression by a Non-Phosphoenolpyruvate-Dependent Phosphotransferase System Sugar (2000)

Streptococcus thermophilus, unlike many other gram-positive bacteria, prefers lactose over glucose as the primary carbon and energy source. Moreover, lactose is not taken up by a phosphoenolpyruvate-dependent phosphotransferase system (PTS) but by the dedicated transporter LacS. In this paper we show that CcpA plays a crucial role in the fine-tuning of lactose transport, beta-galactosidase (LacZ) activity, and glycolysis to yield optimal glycolytic flux and growth rate. A catabolite-responsive element (cre) was identified in the promoter of the lacSZ operon, indicating a possible role for regulation by CcpA. Transcriptional analysis showed a sevenfold relief of repression in the absence of a functional CcpA when cells were grown on lactose. This CcpA-mediated repression of lacSZ transcription did not occur in wild-type cells during growth on galactose, taken up by the same LacS transport system. Lactose transport during fermentation was increased significantly in strains carrying a disrupted ccpA gene. Moreover, a ccpA disruption strain was found to release substantial amounts of glucose into the medium when grown on lactose. Transcriptional analysis of the ldh gene showed that expression was induced twofold during growth on lactose compared to glucose or galactose, in a CcpA-dependent manner. A reduced rate of glycolysis concomitant with an increased lactose transport rate could explain the observed expulsion of glucose in a ccpA disruption mutant. We propose that CcpA in S. thermophilus acts as a catabolic regulator during growth on the preferred non-PTS sugar lactose. In contrast to other bacteria, S. thermophilus possesses an overcapacity for lactose uptake that is repressed by CcpA to match the rate-limiting glycolytic flux.
Tolerability, Pharmacodynamics, and Pharmacokinetics Studies of Depsipeptide (Romidepsin) in Patients with Acute Myelogenous Leukemia or Advanced Myelodysplastic Syndromes (2008)

Depsipeptide therapy can be administered with acceptable short-term toxicity. However, gastrointestinal symptoms and fatigue seem to be treatment-limiting after multiple cycles. Depsipeptide monotherapy has limited clinical activity in unselected AML/MDS patients.
Plasminogen fragment K1–5 improves survival in a murine hepatocellular carcinoma model (2006)

PlgK1-5 and PlgK1-4 effectively inhibited HCC growth. As PlgK1-5 could also prolong the survival time, inducing complete tumour elimination in half of the AdPlgK1-5-treated mice, PlgK1-5 might be the most potential plasminogen fragment for treatment of experimental HCC.
Phase‐sensitive inversion recovery for myocardialT1mapping with motion correction and parametric fitting (2012)

The assessment of myocardial fibrosis and extracellular volume requires accurate estimation of myocardial T1 s. While image acquisition using the modified Look-Locker inversion recovery technique is clinically feasible for myocardial T1 mapping, respiratory motion can limit its applicability. Moreover, the conventional T1 fitting approach using the magnitude inversion recovery images can lead to less stable T1 estimates and increased computational cost. In this article, we propose a novel T1 mapping scheme that is based on phase-sensitive image reconstruction and the restoration of polarity of the MR signal after inversion. The motion correction is achieved by registering the reconstructed images after background phase removal. The restored signal polarity of the inversion recovery signal helps the T1 fitting resulting in improved quality of the T1 map and reducing the computational cost. Quantitative validation on a data cohort of 45 patients proves the robustness of the proposed method against varying image contrast. Compared to the magnitude T1 fitting, the proposed phase-sensitive method leads to less fluctuation in T1 estimates.
Mechanisms and Development of Etch Resistance for Highly Aromatic Monomolecular Etch Masks - Towards Molecular Lithography (2007)

The road map of the semiconductor industry has followed Moores Law over the past few decades. According to Moores Law the number of transistors in an integrated circuit (IC) will double for a minimum component cost every two years. The features made in an IC are produced by photolithography. Industry is now producing devices at the 65 nm node, however, for every deceasing node size, both the materials and processes used are not only difficult but also expensive to develop. Ultimately, the feature size obtainable via photolithography is dependent on the wavelength used in the process. The limitations of photolithography will eventually make Moores Law unsustainable. Therefore, new methodologies of creating features in the semiconductor substrate are desired. \nHere we present a new way to make patterns in silicon (Si) and silicon dioxide (SiO2), molecular lithography. Individual molecules and polymers, in a monolayer, serves directly as the etch mask; eliminating the photolighographic size limitation of light at a specific wavelength. The Ohnishi- and Ring parameter suggests that cyclic carbon rich molecules have a high resistance towards the plasma process, used to create the features in the substrate. Therefore highly aromatic molecules were investigated as candidates for molecular lithography. \nA monolayer of poly cyclic hydrocarbons, fullerene containing polymer, and fullerene molecules were created using the versatile photochemistry of benzophenone as the linker between the substrate and the material. First, a chlorosilane benzophenone derivative was attached to the Si/SiO2 surface. A thin film of the desired material is then created on top of the silane benzophenone layer. Irradiation at ~350 nm excites the benzophenone and reacts with neighboring alkyl chains. After covalent attachment the non-bonded molecules are extracted from the surface using a Soxhlet apparatus. Self-assembly, molecular weight, and wetting properties of the material dictates the features shape and size. These features are then serving as an etchmask in a fluorine plasma. The organic etch resist is then removed either in an oxygen plasma or in a piranha solution. AFM analysis revealed that 3 to 4 nm wide defined structures were obtained using C96 as the etch mask. This is about ten times smaller then industry standards. Also a depth profile of 50 nm, which is the minimum feature depth used in industry, was created using a fullerene containing polymer as the etch mask. Directionality and control over the shape and sizes of the features are naturally critical for implementing this technology in device fabrication. Therefore, alignment of the materials used has also been examined. \nMonolayers of highly stable molecules has successfully been used as etch masks. Further research and development could implement molecular lithography in device fabrication. Self-assembly among other forces would dictate which materials could be used successfully as a molecular resist.
CH3NH3Cl-Assisted One-Step Solution Growth of CH3NH3PbI3: Structure, Charge-Carrier Dynamics, and Photovoltaic Properties of Perovskite Solar Cells (2014)

We demonstrate a novel one-step solution approach to prepare perovskite CH3NH3PbI3 films by adding CH3NH3Cl (or MACl) to the standard CH3NH3PbI3 precursor (equimolar mixture of CH3NH3I and PbI2) solution. The use of MACl strongly affects the crystallization process of forming pure CH3NH3PbI3, leading not only to enhanced absorption of CH3NH3PbI3 but also to significantly improved coverage of CH3NH3PbI3 on a planar substrate. Compared to the standard one-step solution approach for CH3NH3PbI3, using MACl improves the performance of CH3NH3PbI3 solar cells from about 2% to 12% for the planar cell structure and from about 8% to 10% for the mesostructured device architecture. Although we find no significant effect of using MACl on charge transport and recombination in mesostructured perovskite cells, the recombination resistance for planar cells increases by 1–2 orders of magnitude by using MACl. These results suggest that this new one-step solution approach is promising for controlling CH3NH3PbI3 growth to achieve high-performance perovskite solar cells.
Impact of Ningbo Port Logistics on Regional Economic Development of Zhejiang Province (2019)

In related academic circles, the research on port logistics industry and regional economic development has become a hot topic in recent years, and there are already extensive studies about this topic. Taking Ningbo Port as an example, this paper analyzed its logistics development status, and discussed the existing problems. Besides, taking the time series data of Ningbo Port from 2007 to 2016 as sample data, and using the quantitative analysis methods such as correlation analysis and regression analysis, it systematically studied the interaction between the port development and regional economic development. Finally, on the basis of the problems, it came up with some recommendations.
A noninverting genome of a viable herpes simplex virus 1: presence of head-to-tail linkages in packaged genomes and requirements for circularization after infection (1985)

The wild-type herpes simplex virus 1 genome consists of two components, L and S, which invert relative to each other, giving rise to four isomers. Previously we reported the construction of a herpes simplex virus 1 genome, HSV-1(F)I358, from which 15 kilobase pairs of DNA spanning the junction between L and S components were deleted and which no longer inverted (Poffenberger et al., Proc. Natl. Acad. Sci. U.S.A. 80:2690-2694, 1983). Further studies on the structure of HSV-1(F)I358 revealed the presence of two submolar populations among packaged DNA. The first, comprising no more than 10% of total packaged DNA, consisted of defective genomes with a subunit size of 36 kilobase pairs. The results suggest that this population arose by recombination through a directly repeated sequence inserted in place of the deleted L-S junction. The second minor population consisted of HSV-1(F)I358 DNA linked head-to-tail. Analyses of the structure of HSV-1(F)I358 DNA after infection indicated that the fraction of total DNA linked head-to-tail increased to approximately 40 to 50% within 30 min after exposure of cells to virus. The formation of head-to-tail linkages did not require de novo protein synthesis. Our interpretation of the results is that the termini of full-length DNA molecules are held together during packaging, that a small fraction of the termini is covalently linked during or after packaging, and that the remainder is covalently joined after the release of viral DNA from the infecting virus by either host or viral factors introduced into the cell during infection.
Web page scoring systems for horizontal and vertical search (2002)

Page ranking is a fundamental step towards the construction of effective search engines for both generic (horizontal) and focused (vertical) search. Ranking schemes for horizontal search like the PageRank algorithm used by Google operate on the topology of the graph, regardless of the page content. On the other hand, the recent development of vertical portals (vortals) makes it useful to adopt scoring systems focussed on the topic and taking the page content into account.In this paper, we propose a general framework for Web Page Scoring Systems (WPSS) which incorporates and extends many of the relevant models proposed in the literature. Finally, experimental results are given to assess the features of the proposed scoring systems with special emphasis on vertical search.
Protein tyrosine kinase inhibitors in cancer treatment (1997)

Competition in the field of tyrosine kinase inhibitors has increased in the last few years. New receptor and non-receptor tyrosine kinases have been identified as attractive targets for drug discovery programs. A pharmacophore model of the ATP-binding site of the epidermal growth factor-receptor (EGF-R) tyrosine kinase has been developed and successfully used for the rational design of tyrosine kinase inhibitors. Several inhibitor classes containing a phenylamino-pyrimidine moiety in their structure have provided highly selective ATP-competitive tyrosine kinase inhibitors, active in the low nanomolar or even picomolar range, thus proving that the ATP-binding site of tyrosine kinases is an attractive target for the design of anticancer drugs. More and more examples of inhibitors against several tyrosine kinases are being described which, in addition to showing potent and selective in vitro<> activity, exhibit potency in relevant cellular and in vivo models.<> Presently, several tyrosine kinase inhibitors with an interesting in vitro and in vivo profile are in preclinical evaluation and are expected to enter Phase I clinical trials later this year or early next year. This review summarises development of the last two years in the design and biological profiling of tyrosine kinase inhibitors with special focus on the phenylamino-pyrimidine-containing classes of compounds.
Interferons and other regulatory cytokines (1988)

From an Antiviral Factor to a Family of Multifunctional Cytokines The Interferon Gene Family Induction of IFN-+ga+n and IFN-+gb+n Interferon Receptors Interferons as Multifunctional Gene Activators The Antiviral Activity of Interferons The Effects of Interferons on Cell Growth and Division Interferons and Hematopoiesis Modulation of the Expression of the Major Histocompatibility Antigens Macrophages as Interferon Producers and Interferons as Modulators of Macrophage Activity Production of IFN-+gg+n by T Cells and Modulation of T Cell, B Cell, and NK Cell Activity by Interferons The Effects of Interferons on Immediate and Delayed Hypersensitivity Interaction of Interferons, Tumor Necrosis Factor, Interleukin-1, and Interleukin-2 as Part of the Cytokine Network The Effects of Interferons on Tumor Cells The Genetics of Interferon Production and Action The Presence and Possible Pathogenic Role of Interferons in Disease.
Comparative Studies on Structural and Antigenic Properties of Two Serotypes of Infectious Bursal Disease Virus (1988)

The electrophoretic mobilities of the two genome segments and the structural polypeptides of the chicken strain Cu-1 (serotype I) and the turkey isolate 23/82 (serotype II) of infectious bursal disease virus were compared. There is a close antigenic relationship between the smaller of the two major structural proteins (32K) of both strains. Neutralizing monoclonal antibodies are induced by the larger protein (40K in Cu-1) which differentiates between the two serotypes. The 40K structural protein also has epitopes which do not induce neutralizing antibodies and which are common to both strains. There is evidence that the antigenic region responsible for the production of neutralizing antibodies is highly conformation-dependent. Passively administered neutralizing antibodies directed against the 40K structural polypeptide of Cu-1 confer protective immunity to susceptible chickens, whereas antibodies directed against the 32K structural protein do not have any protective effect.