gebhart/patrepeval huggingface dataset:
Three-Dimensional Cardiac Tissue Engineering Using a Thermoresponsive Artificial Extracellular Matrix (2004)

The purpose of this study was to try to reconstitute three-dimensional cardiac tissue using a thermoresponsive artificial extracellular matrix, poly (N-isopropylacrylamide)-grafted gelatin (PNIPAM-gelatin), as the scaffold. PNIPAM-gelatin solution gels almost immediately when heated above 34 degrees C. We thought this property could become advantageous as scaffolding for reconstituting three-dimensional tissue. Because PNIPAM-gelatin solution gels so quickly, all seeded cells in PNIPAM-gelatin solution would become entrapped and uniformly distributed toward three dimensions. Thus it would be possible to reconstitute three-dimensional tissue by a very simple method of mixing cells and PNIPAM-gelatin solution. Fetal rat cardiac cells were mixed with PNIPAM-gelatin solution, incubated at 37 degrees C to allow the mixture to gel, and cultured in vitro. To define suitable culture conditions the following parameters were tested: (1) PNIPAM-gelatin concentration, 0.04 approximately 0.125 mg/ml; (2) cell seeding density, 1 approximately 50 x 10(6) cells/ml; and (3) addition or not of hyaluronic acid. With a PNIPAM-gelatin concentration of 0.05 mg/ml, a cell seeding density of 50 x 10(6) cells/ml, and the addition of hyaluronic acid, tissue was reconstituted and it contracted synchronously. After hematoxylin and eosin staining, the cells reconstituted three-dimensional tissue, and the tissue cross-section was approximately 60 microm thick.
The TRAP220 component of a thyroid hormone receptor- associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion (1998)

Cognate cDNAs are described for 2 of the 10 thyroid hormone receptor-associated proteins (TRAPs) that are immunopurified with thyroid hormone receptor alpha (TRalpha) from ligand-treated HeLa (alpha-2) cells. Both TRAP220 and TRAP100 contain LXXLL domains found in other nuclear receptor-interacting proteins and both appear to reside in a single complex with other TRAPs (in the absence of TR). However, only TRAP220 shows a direct ligand-dependent interaction with TRalpha, and these interactions are mediated through the C terminus of TRalpha and (at least in part) the LXXLL domains of TRAP220. TRAP220 also interacts with other nuclear receptors [vitamin D receptor, retinoic acid receptor alpha, retinoid X receptor alpha, peroxisome proliferation-activated receptor (PPAR) alpha, PPARgamma and, to a lesser extent, estrogen receptor] in a ligand-dependent manner, whereas TRAP100 shows only marginal interactions with estrogen receptor, retinoid X receptor alpha, PPARalpha, and PPARgamma. Consistent with these results, TRAP220 moderately stimulates human TRalpha-mediated transcription in transfected cells, whereas a fragment containing the LXXLL motifs acts as a dominant negative inhibitor of nuclear receptor-mediated transcription both in transfected cells (TRalpha) and in cell free transcription systems (TRalpha and vitamin D receptor). These studies indicate that TRAP220 plays a major role in anchoring other TRAPs to TRalpha during the function of the TRalpha-TRAP complex and, further, that TRAP220 (possibly along with other TRAPs) may be a global coactivator for the nuclear receptor superfamily.
A Graphene Field-Effect Device (2007)

In this letter, a top-gated field-effect device (FED) manufactured from monolayer graphene is investigated. Except for graphene deposition, a conventional top-down CMOS-compatible process flow is applied. Carrier mobilities in graphene pseudo-MOS structures are compared to those obtained from the top-gated Graphene-FEDs. The extracted values exceed the universal mobility of silicon and silicon-on-insulator MOSFETs
Binding of Tenascin-C to Soluble Fibronectin and Matrix Fibrils (1995)

The small splice variant of tenascin-C (TN) has eight fibronectin type III (FN3) domains. The major large splice variant has three (in chicken) or seven (in human) additional FN3 domains inserted between domains five and six. Chiquet-Ehrismann et al. (Chiquet-Ehrismann, R., Matsuoka, Y., Hofer, U., Spring, J., Bernasconi, C., and Chiquet, M. (1991, Cell Regul. 2, 927-938) demonstrated that the small variant bound preferentially to fibronectin in enzyme-linked immunosorbent assay, and only the small variant was incorporated into the matrix by cultures of chicken fibroblasts. Here we have studied human TN, and confirmed that the small variant binds preferentially to purified fibronectin and to fibronectin-containing extracellular matrix. Thus this differential binding appears to be conserved across vertebrate species. Using bacterial expression proteins, we mapped the major binding site to the third FN3 domain of TN. Consistent with this mapping, a monoclonal antibody against an epitope in this domain did not stain TN segments bound to cell culture matrix fibrils. The enhanced binding of the small TN variant suggests the existence of another, weak binding site probably in FN3 domains 6-8, which is only positioned to bind fibronectin in the small splice variant. This binding of domains 6-8 may involve a third molecule present in matrix fibrils, as the enhanced binding of small TN was much more prominent to matrix fibrils than to purified fibronectin.
Preparation and reactivity studies of highly versatile, nickel-based polymerization catalyst systems (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation and reactivity studies of highly versatile, nickel-based polymerization catalyst systemsTimothy J. Deming and Bruce M. NovakCite this: Macromolecules 1993, 26, 25, 7089–7091Publication Date (Print):December 1, 1993Publication History Published online1 May 2002Published inissue 1 December 1993https://pubs.acs.org/doi/10.1021/ma00077a059https://doi.org/10.1021/ma00077a059research-articleACS PublicationsRequest reuse permissionsArticle Views375Altmetric-Citations70LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Coherence and Coreference* (1979)

Coherence in conversations and in texts can be partially characterized by a set of coherence relations, motivated ultimately by the speaker's or writer's need to be understood. In this paper, formal definitions are given for several coherence relations, based on the operations of an inference system; that is, the relations between successive portions of a discourse are characterized in terms of the inferences that can be drawn from each. In analyzing a discourse, it is frequently the case that we would recognize it as coherent, in that it would satisfy the formal definition of some coherence relation, if only we could assume certain noun phrases to be coreferential. In such cases, we will simply assume the identity of the entities referred to, in what might be called a “petty conversational implicature,” thereby solving the coherence and coreference problems simultaneously. Three examples of different kinds of reference problems are presented. In each, it is shown how the coherence of the discourse can be recognized, and how the reference problems are solved, almost as a by‐product, by means of these petty conversational implicatures.
Object browsing and searching in a camera network using graph models (2012)

This paper proposes a novel system to assist human image analysts to effectively browse and search for objects in a camera network. In contrast to the existing approaches that focus on finding global trajectories across cameras, the proposed approach directly models the relationship among raw camera observations. A graph model is proposed to represent detected/tracked objects, their appearance and spatial-temporal relationships. In order to minimize communication requirements, we assume that raw video is processed at camera nodes independently to compute object identities and trajectories at video rate. However, this would result in unreliable object locations and/or trajectories. The proposed graph structure captures the uncertainty in these camera observations by effectively modeling their global relationships, and enables a human analyst to query, browse and search the data collected from the camera network. A novel graph ranking framework is proposed for the search and retrieval task, and the absorbing random walk algorithm is adapted to retrieve a representative and diverse set of video frames from the cameras in response to a user query. Preliminary results on a wide area camera network are presented.
Characterization of a glucocorticoid-sensitive human lymphoid cell line. (1977)

A human lymphoblastoid cell line (CEM), the growth of which is inhibited by glucocorticoids, is described. Although growth of the original uncloned cell line is only slightly retarded by dexamethasone, sensitive clones were isolated in which growth is completely blocked by 2 to 3 days of exposure to 10(-6) M dexamethasone. After 4 to 5 days, these cells become pyknotic and lyse. The inhibitory affect of dexamethasone first become apparent in suspension culture at a concentration of about 5 X 10(-8) M. Receptor analysis showed the presence of specific glucocorticoid receptors with an apparent dissociation constant for dexamethasone of about 1.3 X 10(-8) M both in the sensitive and in one resistant clone analyzed. Ability to displace dexamethasone from the receptor is correlated with the known glucocorticoid activity of all steroids tested, as is their ability to inhibit cloning of sensitive cells in agarose. These results indicate that the specificity of inhibitory effects is related to receptor specificity of inhibitory effects is related to receptor specificity. Dexamethasone is a potent inhibitor when cells are cloned in agarose, having a marked effect even at a concentration of 7 X 10(-9) M. CEM cells thus provide human cell lines suitable for in vitro analysis of steroid effects on leukemic cells.
A RECEPTORS (2001)

RETURN TO ISSUEPREVNewsNEXTA RECEPTORSCompounds that activate or inhibit adenosine A receptors are being studied for potential therapeutic use in heart disease and cancerSTU BORMANView Author Information C&EN WASHINGTONCite this: Chem. Eng. News 2001, 79, 7, 37–40Publication Date (Print):February 12, 2001Publication History Published online13 November 2010Published inissue 12 February 2001https://pubs.acs.org/doi/10.1021/cen-v079n007.p037https://doi.org/10.1021/cen-v079n007.p037newsACS PublicationsCopyright © 2001 American Chemical SocietyArticle Views11Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Nucleic acids,Receptors Get e-Alerts
MILD HEAD INJURY (1992)

We reviewed the records of 1538 mild head injury patients admitted during a 4 1/2-year period to the Southern New Jersey Regional Trauma Center. All patients had experienced brief loss of consciousness or amnesia, but had a normal or near normal neurologic examination on admission, with Glasgow Coma Scale (GCS) scores of 13-15 and no focal neurologic deficit. Routine urgent cranial CT scans were obtained on all patients, and correlations between skull fractures and intracranial lesions investigated. Two hundred sixty-five patients (17.2%) harbored 340 lesions on CT scans, of which 131 were fractures and 209 were intracranial abnormalities. Fifty-eight patients needed surgery for their intracranial lesions; 23 of them had no skull fractures. None of the 1339 patients without CT evidence of intracranial lesions deteriorated under observation. We conclude that clinical observation with or without skull x-ray films is inadequate to rule out potentially dangerous intracranial lesions in apparently mild head injuries. If there is a history of loss of consciousness or amnesia, an immediate CT scan is indicated. If the results of the CT scan are normal and there are no other indications for admission, these patients may be safely discharged.
CRIGOS: a compact robot for image-guided orthopedic surgery (1999)

The CRIGOS (compact robot for image-guided orthopedic surgery) project was set up for the development of a compact surgical robot system for image-guided orthopedic surgery based on user requirements. The modular system comprises a compact parallel robot and a software system for planning of the surgical interventions and for supervision of the robotic device. Because it is not sufficient to consider only technical aspects in order to improve in clinical routine the therapeutic outcome of conventional interventions, a user-centered and task-oriented design process has been developed which also takes human factors into account. The design process for the CRIGOS system was started from requirement analysis of various orthopedic interventions using information gathered from literature, questionnaires, and workshops with domain experts. This resulted in identification of conventional interventions for which the robotic system would improve the medical and procedural quality. A system design concept has been elaborated which includes definitions of components, functionalities, and interfaces. Approaches to the acquisition of calibrated X-rays will be presented in the paper together with design and evaluation of a first human-computer interface. Finally, the first labtype parallel robot based on low-cost standard components is presented together with the first evaluation results concerning positioning accuracy.
Human models of aging and longevity (2008)

No single model can be considered the gold standard for the study of aging and longevity, instead the combination of results obtained from different models must be considered in order to better understand these complex phenomena. We propose that a systems biology concept such as that of 'bow-tie' architecture, useful for managing information flow, could help in this demanding task.
In vitro and in vivo activity of MT201, a fully human monoclonal antibody for pancarcinoma treatment (2002)

In our study, a novel, fully human, recombinant monoclonal antibody of the IgG1 isotype, called MT201, was characterized for its binding properties, complement-dependent (CDC) and antibody-dependent cellular cytotoxicity (ADCC), as well as for its in vivo antitumor activity in a nude mouse model. MT201 was found to bind its target, the epithelial cell adhesion molecule (Ep-CAM; also called 17-1A antigen, KSA, EGP-2, GA733-2), with low affinity in a range similar to that of the clinically validated, murine monoclonal IgG2a antibody edrecolomab (Panorex(R)). MT201 exhibited Ep-CAM-specific CDC with a potency similar to that of edrecolomab. However, the efficacy of ADCC of MT201, as mediated by human immune effector cells, was by 2 orders of magnitude higher than that of edrecolomab. Addition of human serum reduced the ADCC of MT201 while it essentially abolished ADCC of edrecolomab within the concentration range tested. In a nude mouse xenograft model, growth of tumors derived from the human colon carcinoma line HT-29 was significantly and comparably suppressed by MT201 and edrecolomab. The fully human nature and the improved ADCC of MT201 with human effector cells will make MT201 a promising candidate for the clinical development of a novel pan-carcinoma antibody that is superior to edrecolomab.
Cytostatic effects of bleomycin and cis-platinum in A549 human lung tumour cells using autofeeder conditions. (1983)

Residual colony forming ability (CFA) of cells from a human alveolar carcinoma (A549) was determined after 48 hours of contact with bleomycin (BLM) or cisdichlorodiammineplatinum (II) (DDP) using feeder cells arising from parallel cultures left in contact with sublethal BLM or DDP concentrations. The CFA of untreated cells was 60 to 80% (45% with X-irradiated feeder cells). Growing cultures were more resistant to DDP and more sensitive to BLM than plateau phase cultures. Subclones arising from a first treatment were resistant to a second BLM or X-ray treatment, but more sensitive to a second DDP treatment.
Measuring depth depends on frequency in electrical skin impedance measurements (1999)

Background/aims: Multi‐frequency electrical measurements in vivo on stratum corneum are frequently reported in the literature. It is well known that the measured volume of skin is dependent on measuring frequency, and we wanted to investigate to what extent viable skin influences the measured impedance as a function of frequency. Methods: Finite element modelling (FEM) was used to investigate the current and potential distribution in the skin when measuring impedance with two concentric surface electrodes. Results: The study showed that the impedance measured reflects mainly the stratum corneum at low frequencies, in this case below 1 kHz, and that the viable skin dominates at higher frequencies. Conclusion: It is concluded that a multi‐frequency measurement on isolated stratum corneum is not possible in vivo with conventional techniques, except within a very narrow and low frequency range.
Building blocks for protein interaction devices (2010)

Here, we propose a framework for the design of synthetic protein networks from modular protein–protein or protein–peptide interactions and provide a starter toolkit of protein building blocks. Our proof of concept experiments outline a general work flow for part–based protein systems engineering. We streamlined the iterative BioBrick cloning protocol and assembled 25 synthetic multidomain proteins each from seven standardized DNA fragments. A systematic screen revealed two main factors controlling protein expression in Escherichia coli: obstruction of translation initiation by mRNA secondary structure or toxicity of individual domains. Eventually, 13 proteins were purified for further characterization. Starting from well-established biotechnological tools, two general–purpose interaction input and two readout devices were built and characterized in vitro. Constitutive interaction input was achieved with a pair of synthetic leucine zippers. The second interaction was drug-controlled utilizing the rapamycin-induced binding of FRB(T2098L) to FKBP12. The interaction kinetics of both devices were analyzed by surface plasmon resonance. Readout was based on Förster resonance energy transfer between fluorescent proteins and was quantified for various combinations of input and output devices. Our results demonstrate the feasibility of parts-based protein synthetic biology. Additionally, we identify future challenges and limitations of modular design along with approaches to address them.
Rheology of Sodium Hyaluronate under Physiological Conditions (2001)

Sodium hyaluronate NaHA in phosphate-buffered saline behaves as a typical polyelectrolyte in the high-salt limit, as Newtonian viscosities are observed over a wide range of shear rates. There is no evidence of intermolecule hydrogen bonding causing gel formation in NaHA solutions without protein present. The concentration dependences of viscosity, relaxation time, and terminal modulus are consistent with observations on flexible, neutral polymers in good solvents, which are known to be in the same universality class as flexible polyelectrolytes in the presence of excess salt.
Drusen and Disciform Macular Detachment and Degeneration (1973)

Follow-up studies on 200 patients who had macular drusen were done for an average of four years. Loss of central vision was caused by disciform detachment of the pigment epithelium and retina, or less often by geographic atrophy of the pigment epithelium and retina. The average age of onset of loss of central vision in the first eye was 66 years and in the second eye 70 years. No clinical fluorescein angiographic or electrophysiologic criteria were found to differentiate patients with familial from those with so-called senile drusen. probably all patients with macular drusen have the same autosomal dominant heredodegenerative disease, which rarely causes significant loss of central vision prior to the sixth and seventh decades of life. Fifty-three patients were treated with photocoagulation, the value of which is still uncertain.
Signal Processing for FMCW SAR (2007)

The combination of frequency-modulated continuous-wave (FMCW) technology and synthetic aperture radar (SAR) techniques leads to lightweight cost-effective imaging sensors of high resolution. One limiting factor to the use of FMCW sensors is the well-known presence of nonlinearities in the transmitted signal. This results in contrast- and range-resolution degradation, particularly when the system is intended for high-resolution long-range applications, as it is the case for SAR. This paper presents a novel processing solution, which solves the nonlinearity problem for the whole range profile. Additionally, the conventional stop-and-go approximation used in pulse-radar algorithms is not valid in FMCW SAR applications under certain circumstances. Therefore, the motion within the sweep needs to be taken into account. Analytical development of the FMCW SAR signal model, starting from the deramped signal and without using the stop-and-go approximation, is presented in this paper. The model is then applied to stripmap, spotlight, and single-transmitter/multiple-receiver digital-beamforming SAR operational mode. The proposed algorithms are verified by processing real FMCW SAR data collected with the demonstrator system built at the Delft University of Technology.