gebhart/patrepeval huggingface dataset:
Characterization of COX9, the nuclear gene encoding the yeast mitochondrial protein cytochrome c oxidase subunit VIIa. Subunit VIIa lacks a leader peptide and is an essential component of the holoenzyme. (1986)

The gene COX9 for subunit VIIa of cytochrome c oxidase from Saccharomyces cerevisiae has been cloned with the aid of an oligonucleotide probe. From the nucleotide sequence of COX9, we deduce that subunit VIIa is derived from a precursor that is 59 amino acids in length (Mr = 6963). This precursor is longer than mature subunit VIIa by one amino acid at its NH2 terminus and four amino acids at its COOH terminus. COX9 exists as a single copy in the haploid genome of S. cerevisiae and produces one major transcript. When the genomic copy of COX9 is removed, cells lack a functional cytochrome c oxidase holoenzyme. From the predicted secondary structure of subunit VIIa, previous data concerning its relationship to the lipid bilayer of the inner membrane and the location of its hydrophobic domains (Power, S.D., Lochrie, M.A., and Poyton, R.O. (1986) J. Biol. Chem. 261, 9206-9209) and the finding that it is essential for the holoenzyme, we propose a model for subunit VIIa which suggests that this small integral protein plays a role in holoenzyme assembly or stability.
Association between Plasma Homocysteine Concentrations and Extracranial Carotid-Artery Stenosis (1995)

High plasma homocysteine concentrations and low concentrations of folate and vitamin B6, through their role in homocysteine metabolism, are associated with an increased risk of extracranial carotid-artery stenosis in the elderly.
Mobile augmented reality for books on a shelf (2011)

Retrieving information about books on a bookshelf by snapping a photo of book spines with a mobile device is very useful for bookstores, libraries, offices, and homes. In this paper, we develop a new mobile augmented reality system for book spine recognition. Our system achieves very low recognition delays, around 1 second, to support real-time augmentation on a mobile device's viewfinder. We infer user interest by analyzing the motion of objects seen in the viewfinder. Our system initiates a query during each low-motion interval. This selection mechanism eliminates the need to press a but ton and avoids using degraded motion-blurred query frames during high-motion intervals. The viewfinder is augmented with a book's identity, prices from different vendors, average user rating, location within the enclosing bookshelf, and a digital compass marker. We present a new tiled search strategy for finding the location in the bookshelf with improved accuracy in half the time as in a previous state-of-the-art system. Our AR system has been implemented on an Android smartphone.
Generation of Papillomavirus-Immortalized Cell Lines from Normal Human Ectocervical, Endocervical, and Vaginal Epithelium that Maintain Expression of Tissue-Specific Differentiation Proteins1 (1997)

We have established and characterized three cell lines from normal human vaginal, ectocervical, and endocervical epithelia immortalized by expression of human papillomavirus 16/E6E7. The lines (VK2/E6E7, Ect1/E6E7, and End1/E6E7) displayed distinctive morphologies at the level of light microscopy when cultured in calcium-supplemented (0.4 mM) keratinocyte serum-free medium and maintained a stable phenotype after more than 1 yr of continuous passage. They were compared to primary cell cultures and epithelial cells in sections of the respective native tissues for expression of epithelial differentiation proteins. All cell lines expressed cytokeratin (CK) 8, CK18, and CK19, and some cells in all three cell lines expressed CK16, involucrin, and the secretory component of the polymeric immunoglobulin receptor. The vaginal and ectocervical cell lines expressed CK10 and CK13, whereas the endocervical line did not. With the exception of CK8 and CK18 expression, the morphological and immunocytochemical characteristics of the immortalized lines closely resembled those of their respective tissues of origin and primary cultures, and all differed significantly from the HeLa cervical adenocarcinoma cell line. These new cell lines may provide the basis for valid, reproducible in vitro models for studies on cervicovaginal physiology and infections and for testing pharmacological agents for intravaginal application.
Immunolocalization of tissue inhibitor of metalloproteinases (timp) in human cells characterization and use of a specific antiserum (1985)

A specific antiserum to pure human amniotic fluid metalloproteinase inhibitor (TIMP) was raised in a sheep. This antiserum was used to demonstrate: firstly, the immunological identity of the TIMP activities from amniotic fluid and culture medium of human foetal lung fibroblasts; and secondly, by indirect immunofluorescence, the secretion of TIMP by human foetal lung fibroblasts, chondrocytes, epithelial cells and smooth muscle cells. The phorbol ester, 12-O-tetradecanoylphorbol-13-acetate, was used to stimulate secretion of TIMP by human foetal lung fibroblasts and the ionophore monensin was used to demonstrate intracellular accumulation of TIMP in the Golgi apparatus of these cells. These results are discussed in relation to other inhibitors of collagenase reported in the literature, which are probably identical to TIMP.
A novel synthetic oleanane triterpenoid, 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid, with potent differentiating, antiproliferative, and anti-inflammatory activity. (1999)

The new synthetic oleanane triterpenoid 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid (CDDO) is a potent, multifunctional molecule. It induces monocytic differentiation of human myeloid leukemia cells and adipogenic differentiation of mouse 3T3-L1 fibroblasts and enhances the neuronal differentiation of rat PC12 pheochromocytoma cells caused by nerve growth factor. CDDO inhibits proliferation of many human tumor cell lines, including those derived from estrogen receptor-positive and -negative breast carcinomas, myeloid leukemias, and several carcinomas bearing a Smad4 mutation. Furthermore, it suppresses the abilities of various inflammatory cytokines, such as IFN-gamma, interleukin-1, and tumor necrosis factor-alpha, to induce de novo formation of the enzymes inducible nitric oxide synthase (iNos) and inducible cyclooxygenase (COX-2) in mouse peritoneal macrophages, rat brain microglia, and human colon fibroblasts. CDDO will also protect rat brain hippocampal neurons from cell death induced by beta-amyloid. The above activities have been found at concentrations ranging from 10(-6) to 10(-9) M in cell culture, and these results suggest that CDDO needs further study in vivo, for either chemoprevention or chemotherapy of malignancy as well as for neuroprotection.
Sucrose Control of Phytochrome A Signaling in Arabidopsis (1997)

Paul P. Dijkwel, Casper Huijser, Peter J. Weisbeek, Nam-Hai Chua, Sjef C. M. Smeekens, Sucrose Control of Phytochrome A Signaling in Arabidopsis, The Plant Cell, Vol. 9, No. 4 (Apr., 1997), pp. 583-595
A Simple Solid-Phase Radioimmunoassay for Glutamic Acid Decarboxylase(GAD) Antibodies in Patients with Diabetes Mellitus. (1996)

Antibodies to glutamic acid decarboxylase (GAD Ab's) provide an important serologic marker for insulin-dependent diabetes mellitus (IDDM), but the diagnostic sensitivity and specificity of GAD antibodies have yet to be defined in assay systems suitable for screening a large number of samples. Therefore, using affinity purified GAD from rat brain we have developed a simple and sensitive solid-phase radioimmunoassay for measuring antibodies against the isoforms GAD65 and GAD67 and have examined a large number of serum samples from Japanese patients with IDDM of short-duration (less than 3 years) as well as from those with non-insulin-dependent diabetes mellitus (NIDDM). We detected GAD Ab in 62% (37 of 60) of the patients with IDDM, but only in 8% (4 of 51) of those with NIDDM. Almost all the patients with other autoimmune diseases and the healthy controls had no GAD Ab at all. Seventy-eight percent of the IDDM patients with GAD Ab concomitantly had islet cell cytoplasmic antibody (ICA), and there was a significant correlation between both autoantibody levels. This assay provides for rapid and simple screening for the early diagnosis of IDDM.
Performance Characteristics of a Cystatin C Immunoassay with Avian Antibodies (2007)

Gentian cystatin C PETIA is shown to have excellent performance between methods . Interference results are improved due to avian antibodies and a broader span of the calibration curve. Avian antibodies are also known to have better immune response than mammalian antibodies towards mammalian antigens.
Software Reflexion Models: Bridging the Gap between Source and High-Level Models (1995)

Software engineers often use high-level models (for instance, box and arrow sketches) to reason and communicate about an existing software system. One problem with high-level models is that they are almost always inaccurate with respect to the system&amp;apos;s source code. We have developed an approach that helps an engineer use a high-level model of the structure of an existing software system as a lens through which to see a model of that system&amp;apos;s source code. In particular, an engineer defines a high-level model and specifies how the model maps to the source. A tool then computes a software reflexion model that shows where the engineer&amp;apos;s high-level model agrees with and where it differs from a model of the source. The paper provides a formal characterization of reflexion models, discusses practical aspects of the approach, and relates experiences of applying the approach and tools to a number of different systems. The illustrative example used in the paper describes the application of refle...
SEQUENCES OF INTEREST: Complementary Deoxyribonucleic Acid Cloning of a Novel Transforming Growth Factor-β Messenger Ribonucleic Acid from Chick Embryo Chondrocytes (1988)

Transforming growth factor beta 1 (TGF beta 1) has been purified and the mRNA cloned from a number of mammalian species including human, murine, bovine, porcine, and simian. Using a human TGF beta 1 cDNA probe, we have detected two distinct TGF beta RNAs in cultured primary chick embryo chondrocytes. One of these RNAs, migrating at about 1.7 kilobases, shows similarity to mammalian TGF beta 1. The second RNA, migrating at about 3 kilobases, is a novel TGF beta mRNA which we have named TGF beta 3. Clones corresponding to each of these RNAs were isolated from a cultured primary chick embryo chondrocyte cDNA library. Two cDNA clones for TGF beta 3, pTGFB-ChX17 and pTGFB-ChX25, contained a 39 nucleotide-long 5'-untranslated region, a 1236 nucleotide-long coding region, and a 911 nucleotide-long 3'-untranslated region. The predicted protein includes a signal peptide of 20-23 amino acids as in human TGF beta 1 and 2, and a precursor protein consisting of 412 amino acids, which can be cleaved at a lys-arg site to produce a 112 amino acid processed peptide containing nine cysteine residues in the same positions as in human TGF beta 1 and 2. At the nucleotide level, the processed coding region of TGF beta 3 shows 72% and 76% identity with the processed coding regions of human TGF beta 1 and TGF beta 2, respectively; at the amino acid level, TGF beta 3 shows 76% identity with TGF beta 1 and 79% identity with TGF beta 2.(ABSTRACT TRUNCATED AT 250 WORDS)
Diferencias de género en la salud autopercibida en Corea del Sur en comparación con Estados Unidos (2016)

Se abordan las diferencias de genero como clave para la reduccion de la brecha de salud. Este estudio se plantea describir las diferencias de genero en salud autoinformada en Corea del Sur y Estados Unidos. Un total de 33.240 participantes de los KNHNES y 39.646 de la NHNES se incluyeron en el estudio. Se realizo un analisis de regresion logistica multiple para identificar las diferencias de genero en salud autoinformada. Esta fue calificada como pobre por el 18,8% y 16,3% de los participantes en Corea del Sur y en los Estados Unidos, respectivamente. Los resultados indicaron que las mujeres de Corea del Sur tienen mayor riesgo de mala salud autoinformada, difiriendo de las mujeres estadounidenses. El grupo de edad 20-39 anos tuvo un mayor riesgo de mala salud autoinformada, tanto en Corea del Sur como en Estados Unidos. Se sugiere que los roles de genero tradicionales de Corea del Sur afectan negativamente a las mujeres. Por lo tanto, el bienestar de Corea del Sur debe ser mejorado para reducir estas brechas de salud entre paises mediante la aplicacion de leyes que tengan en cuenta el genero y el grupo de edad de los beneficiarios.
Adult onset immunodeficiency caused by inherited adenosine deaminase deficiency. (1994)

Adenosine deaminase (ADA) deficiency is identified here as a cause of adult onset immunodeficiency. Two sisters who noted recurrent, predominantly chest infections in their twenties were found in their thirties to have CD4+ lymphopenia and lymphocyte ADA activity of approximately 5% of the lower limit of normal. Immune function, measured by proliferation of PBMCs in vitro to mitogens and specific Ags, was impaired. Inheritance of a polymorphic marker showed that both patients were heterozygous at the ADA locus. In the paternal allele there was a deletion resulting from homologous recombination between two alu elements that normally flank the first exon and the polymorphic marker. The recombination site was distinct from that in similar deletions described in two infants having severe combined immunodeficiency. This allele is predicted to result in a null phenotype. In the mutant allele inherited from the mother, a C to T transition in a CpG dinucleotide changed the codon for arginine 211, which lies in a conserved sequence close to the active site, to that for cysteine. This mutation has been observed previously in a child in whom the other allele was also a null mutation, but who was diagnosed as having partial ADA deficiency because immune function was apparently normal. The late onset of immunodeficiency in our patients suggests that immune function in children with partial ADA deficiency may deteriorate with time and that ADA deficiency should be regarded as a possible cause of adult onset immune dysfunction of unknown etiology.
GroupLens (1997)

article Free Access Share on GroupLens: applying collaborative filtering to Usenet news Authors: Joseph A. Konstan Computer Science Department, The University of Minnesota, Minneapolis and Net Perceptions, Eden Prairie, Minn. Computer Science Department, The University of Minnesota, Minneapolis and Net Perceptions, Eden Prairie, Minn.View Profile , Bradley N. Miller Net Perceptions, Inc., Eden Prairie, Minn. Net Perceptions, Inc., Eden Prairie, Minn.View Profile , David Maltz Computer Science Department, Carnegie Mellon University Computer Science Department, Carnegie Mellon UniversityView Profile , Jonathan L. Herlocker computer science Department, The University of Minnesota computer science Department, The University of MinnesotaView Profile , Lee R. Gordon Net Perceptions, Inc., Eden Prairie, Minn. and The University of Minnesota, Minneapolis Net Perceptions, Inc., Eden Prairie, Minn. and The University of Minnesota, MinneapolisView Profile , John Riedl computer Science Department, University of Minnesota, Net Perceptions, Inc. computer Science Department, University of Minnesota, Net Perceptions, Inc.View Profile Authors Info & Claims Communications of the ACMVolume 40Issue 3March 1997 pp 77–87https://doi.org/10.1145/245108.245126Published:01 March 1997Publication History 1,710citation7,062DownloadsMetricsTotal Citations1,710Total Downloads7,062Last 12 Months602Last 6 weeks78 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Cytochrome c Release and Apoptosis Induced by Mitochondrial Targeting of Nuclear Orphan Receptor TR3 (2000)

TR3, an immediate-early response gene and an orphan member of the steroid-thyroid hormone-retinoid receptor superfamily of transcription factors, regulates apoptosis through an unknown mechanism. In response to apoptotic stimuli, TR3 translocates from the nucleus to mitochondria to induce cytochrome c release and apoptosis. Mitochondrial targeting of TR3, but not its DNA binding and transactivation, is essential for its proapoptotic effect. Our results reveal a mechanism by which a nuclear transcription factor translocates to mitochondria to initiate apoptosis.
Protein localization by fluorescent microscopy : a practical approach (2000)

Preface 1. Basic Immunofluorescence 2. Immunofluorescent Labelling of Sections 4. Instruments for Fluorescence Imaging 5. Fluorescence Microscopy of Living Vertebrate Cells 6. Visualising Fluorescence in Drosophila - optimal detection in thick specimens 7. Green Fluorescent Protein in Plants 8. Fluorescence Microscopy in Yeast Index
Identification of the Cystic Fibrosis Gene: Chromosome Walking and Jumping (1989)

An understanding of the basic defect in the inherited disorder cystic fibrosis requires cloning of the cystic fibrosis gene and definition of its protein product. In the absence of direct functional information, chromosomal map position is a guide for locating the gene. Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than 500,000 base pairs, from the cystic fibrosis region on the long arm of human chromosome 7. Several transcribed sequences and conserved segments were identified in this cloned region. One of these corresponds to the cystic fibrosis gene and spans approximately 250,000 base pairs of genomic DNA.
Oxidation of wool: the lead acetate test for hydrogen peroxide bleached wool (1936)

The lead acetate test for distinguishing between untreated and hydrogen peroxide bleached wool has been investigated. The degree of darkening of the wool during lead acetate treatments is a function of pH, the maximum difference between untreated and bleached wool occurring at pH 5. The results indicate that hydrogen peroxide partially oxidizes the sulfur in wool and prevents the formation of lead sulfide. A mechanism by which lead sulfide is formed in untreated wool is described.
Low Abundance of Circulating Tumor DNA in Localized Prostate Cancer (2019)

Our findings demonstrate clear differences between localized and advanced prostate cancer with respect to the dissemination and detectability of ctDNA. Because allele-specific alterations in ctDNA are below the threshold for detection in localized prostate cancer, other approaches to identify cell-free nucleic acids of tumor origin may demonstrate better specificity for aggressive disease.
Extraction of symmetry properties using correlation with rotated and reflected images (1993)

Abstract The symmetrical properties of an image or a figure are important in visual psychology and computer vision. This paper proposes a new method to extract the properties of rotational symmetry and reflectional symmetry from planar two‐dimensional images. In the detection of the rotational symmetry, first, the original image for a quantized range of angles of rotation is rotated. The rotated results are convolved with the image using directional features. Then a set of affine transformations is applied to the parameter space to obtain the correlation results using each pixel as the center of rotation. The periodicity of the correlation along the rotation angle for each point is evaluated. Finally, the local maximum correlation is determined that indicates the center and the number of folds of the rotational symmetry. In the detection of the reflectional symmetry, the image for the lines in the possible quantized directions is reflected. Then the correlation between the resulting images and the original image is calculated. The transformation that maximizes the correlation directly indicates the direction of the axis of the reflectional symmetry and the translation amount of the glide symmetry. This method does not require any segmentation nor knowledge of the centroid position. The method in this paper evaluates the transformation that matches the images even though the match is a partial one; thus, it can extract the symmetrical property that exists only partially. Although a large amount of computation and storage capacity is required, the method is fundamental, versatile, and suited to the parallel computation.
Genome-wide gene expression profiles of thyroid carcinoma: Identification of molecular targets for treatment of thyroid carcinoma (2008)

In order to clarify the molecular mechanism involved in thyroid carcinogenesis and to identify candidate molecular targets for diagnosis and treatment, we analyzed genome-wide gene expression profiles of 18 papillary thyroid carcinomas with a microarray representing 38,500 genes in combination with laser microbeam microdissection. We identified 243 transcripts that were commonly up-regulated and 138 transcripts that were down-regulated in thyroid carcinoma. Among these 243 transcripts identified, only 71 transcripts were reported as up-regulated genes in previous microarray studies, in which bulk cancer tissues and normal thyroid tissues were used for the analysis. We further selected genes that were overexpressed very commonly in thyroid carcinoma, though were not expressed in the normal human tissues examined. Among them, we focused on the regulator of G-protein signaling 4 (RGS4) and knocked-down its expression in thyroid cancer cells by small-interfering RNA. The effective down-regulation of its expression levels in thyroid cancer cells significantly attenuated viability of thyroid cancer cells, indicating the significant role of RGS4 in thyroid carcinogenesis. Our data should be helpful for a better understanding of the tumorigenesis of thyroid cancer and could contribute to the development of diagnostic tumor markers and molecular-targeting therapy for patients with thyroid cancer.
Genetic Relationships of Soybean Mutants for Different Linolenic Acid Contents (1998)

The normal content (80–90 g kg −1 ) of linolenic acid in soybean [ Glycine max (L.) Merr.] oil adversely affects oil flavor and stability. A new mutant (M‐24) with lower linolenic acid content (62 g kg −1 ) was developed by x‐ray irradiation. Our objective was to determine the inheritance of linolenic acid content in M‐24 and to determine the genetic relationships of this trait with the fan and fanx loci known to control linolenica acid in M‐5 and KL‐8 mutant, respectively. Reciprocal crosses were made between each mutant and ‘Bay’, and among the three mutants No maternal or cytoplasmic effects were observed in any of the crosses. Data from F 2 seeds of the cross M‐24 ✕ Bay indicated that linolenic acid content in M‐24 was controlled by an allele at a single locus with no dominance effects. In the cross of M‐24 ✕ KL‐8, F 2 segregation indicated that linolenic acid content in M‐24 and KL‐8 was controlled by two different alleles at the same locus. Fort he M‐5 ✕ M‐24 cross, F 2 segregation patterns and the segregation of F 3 seeds from individual F 2 plants indicated that M‐5 and M‐24 mutants have alleles at different independent loci that control linolenic acid content. Therefore, the allele in M‐24 is designated as fanx α (M‐24) to distinguish it from those of fan (M‐5) and fanx (KL‐8). The segregate with the fanfanfanx α fanx α genotype can be considered as an important germplatsm that can reduce the linolenic acid content in soybean oil.