gebhart/patrepeval huggingface dataset:
Cell differentiation in Dictyostelium under submerged conditions. (1977)

Hitherto it has not been possible to obtain spore and stalk cell differentiation of the cellular slime molds in submerged cultures. It is shown here that cells, when placed in roller tubes under an atmosphere of oxygen, will form clumps and differentiate in 48-72 hr into mature spores and stalk cells. Although differentiation occurs without the normal morphogenetic movements, there is the appearance of an anteroposterior polarity of the cells in the clump. In addition to oxygen we examined a number of other factors that affect differentiation.
Patterned orocutaneous therapy improves sucking and oral feeding in preterm infants (2008)

The NTrainer patterned orocutaneous therapy effectively accelerates non-nutritive suck development and oral feeding success in preterm infants who are at risk for oromotor dysfunction.
Epitaxial Thin Films of ATiO3–xHx (A = Ba, Sr, Ca) with Metallic Conductivity (2012)

Epitaxial thin films of titanium perovskite oxyhydride ATiO(3-x)H(x) (A = Ba, Sr, Ca) were prepared by CaH(2) reduction of epitaxial ATiO(3) thin films deposited on a (LaAlO(3))(0.3)(SrAl(0.5)Ta(0.5)O(3))(0.7) substrate. Secondary ion mass spectroscopy detected a substantial amount and uniform distribution of hydride within the film. SrTiO(3)/LSAT thin film hydridized at 530 °C for 1 day had hydride concentration of 4.0 × 10(21) atoms/cm(3) (i.e., SrTiO(2.75)H(0.25)). The electric resistivity of all the ATiO(3-x)H(x) films exhibited metallic (positive) temperature dependence, as opposed to negative as in BaTiO(3-x)H(x) powder, revealing that ATiO(3-x)H(x) are intrinsically metallic, with high conductivity of 10(2)-10(4) S/cm. Treatment with D(2) gas results in hydride/deuteride exchange of the films; these films should be valuable in further studies on hydride diffusion kinetics. Combined with the materials' inherent high electronic conductivity, new mixed electron/hydride ion conductors may also be possible.
Localized CO 2 -laser treatment for mitigation of 351-nm damage growth in fused silica (2002)

A technique for inhibiting the growth of laser-induced surface damage on fused silica, initiated and propagated at the 351-nm laser wavelength, has been investigated. The technique exposes the damage sites to single pulses of a CO<SUB>2</SUB> laser operating at the 10.6 micrometers wavelength at or near beam focus. This method results in a very localized treatment of the laser damage site and modifies the site such that laser damage does not propagate further. A laser damage site initiated with a single pulse of 355-nm laser light at approximately 45 J cm<SUP>-2</SUP> and 7.5-ns pulse duration grows rapidly upon further illumination at 8 J cm<SUP>-2</SUP> with 100% probability. Treatment of these sites with single pulses of 10.6 micrometers laser light for one second at a power level of between 17 and 37 Watts with a beam diameter of 5 mm alters the damage site such that it does not grow with subsequent 351-nm laser illumination at 8 J cm<SUP>-2</SUP> 10-ns pulse duration for > 1000 shots. The technique has been found to be 100% effective at stopping the growth of the laser damage.
Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: characteristics of the model (1976)

Although the suckling mouse assay is widely used for the detection of heat-stable Escherichia coli enterotoxin (ST), few data have been published concerning the reproducibility, optimal growth, and test conditions of this assay. Four strains of toxigenic E. coli known to elaborate both heat-labile enterotoxin and ST or ST alone were used to study these parameters. ST activity after heat treatment and the effect of purified choleragen were also examined. ST production was optimal in Casamino Acids-yeast extract media, but both Trypticase soy and brain heart infusion broths resulted in several false negative reactions. Growing cultures in roller tubes was the most reliable method of ST production. Shaking-flask cultures and stationary-grown cultures resulted in suboptimal ST production in several strains. Optimal mouse incubation time was 3 h, and fluid secretion did not rise thereafter. Adequate toxin production occurred after 16 to 24 h of incubation. The coefficient of variation of various toxins tested on many occasions varied between 10.5 and 15.7%. Toxin activity was stable for 6 months when frozen at - 20 C. There was no decrease in ST activity when heated at 65 C for 15 min, but a small decrease was observed in two of four strains after heating at 100 C for 30 min. Choleragen, tested at various doses and at multiple times, gave uniformly negative results. These studies indicate that when done under the proper conditions, the suckling mouse assay is a simple, rapid, and reproducible assay for E. coli ST.
The age of the universe, dark matter, and structure formation (1998)

The National Academy of Sciences (NAS) sponsored a 2-day colloquium titled “The Age of the Universe, Dark Matter, and Structure Formation,” March 21–23, 1997, at the Arnold and Mabel Beckman Center of the National Academies of Science and Engineering in Irvine, CA. Eighty-eight cosmologists who represented a wide range of individual scientific disciplines attended the colloquium. Also present were two science reporters. The purpose of the colloquium was to address the three interconnected problems that have the center stage in modern physical cosmology today. They are: ( i ) the age of the universe, ( ii …
A Novel Peptide-IgG Conjugate, CAP18106-138-IgG, that Binds and Neutralizes Endotoxin and Kills Gram-Negative Bacteria (1997)

Although type-specific IgG directed to the O-polysaccharide antigen of bacterial lipopolysaccharide (LPS) is protective in most models of LPS or bacterial challenge, no currently available IgG binds to LPS from all gram-negative bacteria. The ability of a peptide-IgG conjugate, CAP18(106-138)-IgG, to bind and neutralize LPS, to kill gram-negative bacteria, and to protect in a sensitized mouse model of LPS toxicity was studied. CAP18(106-138)-IgG bound LPS from multiple gram-negative bacteria in four different binding assays. In a fluid-phase RIA, half-maximal binding of 5 microg/mL 3H-labeled LPS occurred at 5-10 microg/mL CAP18(106-138)-IgG, similar to binding with monoclonal type-specific IgG. CAP18(106-138)-IgG neutralized LPS, as assessed by LPS-induced coagulation of limulus amebocyte lysate and production of tumor necrosis factor in vitro, was bactericidal for a wide range of gram-negative bacteria, and decreased LPS-induced lethality in sensitized mice. Antibacterial peptide-IgG conjugates merit further study as a novel adjunctive therapy for gram-negative sepsis.
Constitutively active mutants of the alpha 2-adrenergic receptor (1993)

We have mutated a single residue, Thr373 [corrected], in the C-terminal portion of the third intracellular loop of the alpha 2C10-adrenergic receptor into five different amino acids. In analogy with the effect of similar mutations in the alpha 1B- and beta 2-adrenergic receptors, these substitutions resulted in two major biochemical modifications: 1) increased constitutive activity of the alpha 2-adrenergic receptor leading to agonist-independent inhibition of adenylyl cyclase and 2) increased affinity of the receptor for binding agonist but not antagonists. The increased constitutive activity of the mutated alpha 2-adrenergic receptors could be inhibited by pertussis toxin, clearly indicating that it results from spontaneous ligand-independent receptor coupling to Gi. In contrast, the increased affinity of the mutant receptors for binding agonists was unaffected by pertussis toxin treatment, indicating that this is an inherent property of the receptors not dependent on interaction with Gi. Coexpression of the receptor mutants with the receptor-specific kinase, beta ARK1, indicated that the constitutively active alpha 2-adrenergic receptors are substrates for beta-adrenergic receptor kinase (beta ARK)-mediated phosphorylation even in the absence of agonist. These findings strengthen the idea that constitutively active adrenergic receptors mimic the "active" state of a G protein-coupled receptor adopting conformations similar to those induced by agonist when it binds to wild type receptors. In addition, these results extend the notion that in the adrenergic receptor family the C-terminal portion of the third intracellular loop plays a general role in the processes involved in receptor activation.
MYC Can Induce DNA Breaks In vivo and In vitro Independent of Reactive Oxygen Species (2006)

MYC overexpression is thought to initiate tumorigenesis by inducing cellular proliferation and growth and to be restrained from causing tumorigenesis by inducing cell cycle arrest, cellular senescence, and/or apoptosis. Here we show that MYC can induce DNA breaks both in vitro and in vivo independent of increased production of reactive oxygen species (ROS). We provide an insight into the specific circumstances under which MYC generates ROS in vitro and propose a possible mechanism. We found that MYC induces DNA double-strand breaks (DSBs) independent of ROS production in murine lymphocytes in vivo as well as in normal human foreskin fibroblasts (NHFs) in vitro in normal (10%) serum, as measured by gammaH2AX staining. However, NHFs cultured in vitro in low serum (0.05%) and/or ambient oxygen saturation resulted in ROS-associated oxidative damage and DNA single-strand breaks (SSBs), as measured by Ape-1 staining. In NHFs cultured in low versus normal serum, MYC induced increased expression of CYP2C9, a gene product well known to be associated with ROS production. Specific inhibition of CYP2C9 by small interfering RNA was shown to partially inhibit MYC-induced ROS production. Hence, MYC overexpression can induce ROS and SSBs under some conditions, but generally induces widespread DSBs in vivo and in vitro independent of ROS production.
Composition and Antimicrobial Activity of the Essential Oils of Micromeria cristata subsp. phrygia and the Enantiomeric Distribution of Borneol (2001)

Water-distilled essential oils from herbal parts of Micromeria cristata (Hampe) Griseb. subsp. phrygia P. H. Davis (Endemic) (Lamiaceae) collected from three different localities were analyzed by GC-MS. The major component characterized in the three oils was borneol (27-39%). Other main components were determined as camphor (9-15%), caryophyllene oxide (4-6%), and trans-verbenol (4-6%) in the oils. Enantiomeric distributions of borneol and camphor in the oils were determined on a fused silica Lipodex-E capillary column using a multidimensional GC-MS system. The three essential oils and both enantiomers of borneol have been evaluated for their antimicrobial activity. They showed inhibitory effects on Gr (-) and Gr (+) pathogenic microorganisms.
Neue Enzyminhibitoren aus Mikroorganismen (1979)

A literature survey on the structures and specificities of enzyme inhibitors isolated from microorganisms is presented, with special emphasis on protease inhibitors and inhibitors of adrenaline metabolism.
Monoclonal Antibodies for Non‐Hodgkin′s Lymphoma: State of the Art and Perspectives (2010)

Monoclonal antibodies have been the most successful therapeutics ever brought to cancer treatment by immune technologies. The use of monoclonal antibodies in B-cell Non-Hodgkin's lymphomas (NHL) represents the greatest example of these advances, as the introduction of the anti-CD20 antibody rituximab has had a dramatic impact on how we treat this group of diseases today. Despite this success, several questions about how to optimize the use of monoclonal antibodies in NHL remain open. The best administration schedules, as well as the optimal duration of rituximab treatment, have yet to be determined. A deeper knowledge of the mechanisms underlying resistance to rituximab is also necessary in order to improve the activity of this and of similar therapeutics. Finally, new antibodies and biological agents are entering the scene and their advantages over rituximab will have to be assessed. We will discuss these issues and present an overview of the most significant clinical studies with monoclonal antibodies for NHL treatment carried out to date.
Local drug delivery (2000)

Intensive research efforts are now focused on the development of new strategies for more effective delivery of drugs to the central nervous system. These strategies include chemical modification of drugs, disruption of the blood-brain barrier, and utilization of alternative routes for drug delivery. This paper focuses on local drug delivery for the treatment of brain tumors. It reviews papers published in the past year on local chemotherapy and immunotherapy. Other aspects of local drug delivery are discussed, including convection-enhanced delivery and drug delivery via a controlled-release microchip.
Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries (2018)

This article provides a status report on the global burden of cancer worldwide using the GLOBOCAN 2018 estimates of cancer incidence and mortality produced by the International Agency for Research on Cancer, with a focus on geographic variability across 20 world regions. There will be an estimated 18.1 million new cancer cases (17.0 million excluding nonmelanoma skin cancer) and 9.6 million cancer deaths (9.5 million excluding nonmelanoma skin cancer) in 2018. In both sexes combined, lung cancer is the most commonly diagnosed cancer (11.6% of the total cases) and the leading cause of cancer death (18.4% of the total cancer deaths), closely followed by female breast cancer (11.6%), prostate cancer (7.1%), and colorectal cancer (6.1%) for incidence and colorectal cancer (9.2%), stomach cancer (8.2%), and liver cancer (8.2%) for mortality. Lung cancer is the most frequent cancer and the leading cause of cancer death among males, followed by prostate and colorectal cancer (for incidence) and liver and stomach cancer (for mortality). Among females, breast cancer is the most commonly diagnosed cancer and the leading cause of cancer death, followed by colorectal and lung cancer (for incidence), and vice versa (for mortality); cervical cancer ranks fourth for both incidence and mortality. The most frequently diagnosed cancer and the leading cause of cancer death, however, substantially vary across countries and within each country depending on the degree of economic development and associated social and life style factors. It is noteworthy that high-quality cancer registry data, the basis for planning and implementing evidence-based cancer control programs, are not available in most low- and middle-income countries. The Global Initiative for Cancer Registry Development is an international partnership that supports better estimation, as well as the collection and use of local data, to prioritize and evaluate national cancer control efforts. CA: A Cancer Journal for Clinicians 2018;0:1-31. © 2018 American Cancer Society.
Diabetic Retinopathy: Mitochondrial Dysfunction and Retinal Capillary Cell Death (2005)

Oxidative stress is increased in the retina in diabetes; the levels of oxidatively modified DNA and nitrosylated proteins are elevated, and antioxidant defense enzymes are impaired. The levels of superoxides are elevated in the retina, and the mitochondria become dysfunctional with proapoptotic protein, Bax, translocating from the cytosol into the mitochondria, and cytochrome c leaking out from the mitochondria. This is accompanied by increased retinal capillary cell apoptosis, and the formation of acellular capillaries and pericyte ghosts, the early signs of retinopathy in animal models of diabetic retinopathy. Inhibition of superoxides inhibits glucose -induced mitochondrial dysfunction, activation of caspase-3, and cell death in retinal capillary cells. In animal models, long-term administration of lipoic acid or other antioxidants inhibits the development of diabetic retinopathy via inhibition of accumulation of oxidatively modified DNA and nitrotyrosine and capillary cell apoptosis in the retina. Understanding the role of mitochondria in the development of retinopathy in diabetes should help identify therapies that can neutralize superoxides and inhibit their dysfunction and, ultimately, the development of retinopathy.
Isolated dual trapped ion cell assembly for Fourier transform ion cyclotron resonance mass spectrometry (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIsolated dual trapped ion cell assembly for Fourier transform ion cyclotron resonance mass spectrometrySteven A. Hofstadler and David A. LaudeCite this: Anal. Chem. 1991, 63, 18, 2001–2007Publication Date (Print):September 15, 1991Publication History Published online1 May 2002Published inissue 15 September 1991https://pubs.acs.org/doi/10.1021/ac00018a019https://doi.org/10.1021/ac00018a019research-articleACS PublicationsRequest reuse permissionsArticle Views38Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Post‐transcriptional gene silencing of ACC synthase in tomato results from cytoplasmic RNA degradation (1997)

Summary Transgenic tomato plants with the coding region of a predominantly fruit specific ACC synthase gene ( LE‐ACS2 ) driven by the CAMV35S promoter, fall into two phenotypic classes: (i) epinastic plants and (ii) plants which appear vegetatively normal with ripening impaired fruit. In leaf tissue from epinastic plants the transgene RNA pool is high, and in the vegetatively normal plants the transgene transcript level is very low. While the epinastic phenotype results from the expected high level expression of the ACC synthase transgene throughout the plant, the vegetatively normal, ripening impaired phenotype is a consequence of the silencing of both the transgene and the corresponding endogenous gene. As in several other cases of gene silencing, the mechanism is post‐transcriptional. Transgene RNA pools are similar in nuclei of epinastic and vegetatively normal tissue. However, the transgene mRNA pool is dramatically reduced in the cytoplasm of vegetatively normal plants, and furthermore, this reduction results from degradation from the 3′ end of the transcript. This degradation probably occurs on the polysomes where the transgene transcripts are localized.
Using Standardized Serum Creatinine Values in the Modification of Diet in Renal Disease Study Equation for Estimating Glomerular Filtration Rate (2006)

The 4-variable MDRD Study equation provides reasonably accurate GFR estimates in patients with chronic kidney disease and a measured GFR of less than 90 mL/min per 1.73 m2. By using the reexpressed MDRD Study equation with the standardized serum creatinine assay, clinical laboratories can report more accurate GFR estimates.
Enhanced two-photon biosensing with double-clad photonic crystal fibers (2003)

A double-clad photonic crystal fiber was used to improve detection efficiency over a standard single-mode fiber in a two-photon fluorescence detection scheme in which the dye was excited and the fluorescence was detected back through the same fiber.
DLR-Hand II: next generation of a dextrous robot hand (2002)

This paper outlines the 2nd generation of multisensory hand design at DLR, based on the results of the DLR Hand I we analysed. An open skeleton structure for better maintenance with semi-shell housing and the new automatically reconfigurable palm have been equipped with more powerful actuators to reach 30 N on the fingertip. The newly designed sensors as the 6-DOF fingertip force torque sensor, the integrated electronics and the new communication architecture with a reduction of cabling to the hand to only 12 lines, are outlined. The Cartesian impedance control of all the fingers completes the new 13-DOF hand.
A grid simulator with control of single-phase power converters in D-Q rotating frame (2003)

A control method in the direct-quadrature (DQ) rotating frame for a single-phase inverter or PFC rectifier is proposed to provide an infinite control gain (theoretically), thus zero steady state error at its fundamental frequency to achieve superior steady state and dynamic performance. This new control method transforms the physical "real circuit", in conjunction with an "imaginary orthogonal circuit", from the stationary frame to the DQ rotating frame so that the steady state voltage/current in the DQ frame become DC variables. The new control concept is validated through a single-phase grid simulator used for utility compatibility tests, as described in IEEE P1547 draft standard, IEEE Std 929, UL 1741 and NYSIR, for single-phase distributed generators (DG), such as residential fuel cell generators or photovoltaic power conditioning systems. Experimental results in steady state with programmable simulated utility line impedance are given along with the dynamic transient response for NYSIR type test. The control concept is also applicable to zero-sequence control for three-phase four-wire systems.
Oral solution of levodopa ethylester for treatment of response fluctuations in patients with advanced Parkinson's disease (2002)

Levodopa ethylester (LDEE), a highly soluble prodrug of levodopa, may overcome the impaired absorption of regular levodopa, due mainly to a combination of levodopa's poor solubility and delayed gastric emptying. We conducted a double-blind, levodopa-controlled, multicenter study of oral LDEE solution compared with standard levodopa-carbidopa (LD-CD) tablets. Sixty-two patients with Parkinson's disease who had "delayed on" and "no-on" subtypes of response fluctuations were randomly assigned for treatment with LDEE-CD or LD-CD 250/25 mg for 4 weeks (phase A). Only the first morning and first post-lunch dose of LD were replaced. This was followed by a 2-week extension with a supplementation of carbidopa (25 mg) to each replaced dose (phase B). Patients filled home diaries 2 weeks before and during the trial period in which times of turning on and off for the two doses were reported. In phase A, mean latency to turning on was reduced by 21% (morning dose) and 17% (post-lunch dose) in the LDEE-CD group. Percentage of no-on episodes after the post-lunch dose was decreased by 21% in the LDEE-CD group but increased by 36% in the LD-CD group (P < 0.01). In phase B, LDEE-CD decreased latencies to on after the morning and post-lunch doses and no-on episodes after the post-lunch dose. The beneficial effects of LDEE were supported by the pharmacokinetic data. Results indicate that LDEE solution is beneficial in ameliorating delayed on and no-on response fluctuations. This effect of LDEE is due to more rapid levodopa absorption.