gebhart/patrepeval huggingface dataset:
Endothelial progenitor cells: identity defined? (2008)

In the past decade, researchers have gained important insights on the role of bone marrow (BM)-derived cells in adult neovascularization. A subset of BM-derived cells, called endothelial progenitor cells (EPCs), has been of particular interest, as these cells were suggested to home to sites of neovascularization and neoendothelialization and differentiate into endothelial cells (ECs) in situ, a process referred to as postnatal vasculogenesis. Therefore, EPCs were proposed as a potential regenerative tool for treating human vascular disease and a possible target to restrict vessel growth in tumour pathology. However, conflicting results have been reported in the field, and the identification, characterization, and exact role of EPCs in vascular biology is still a subject of much discussion. The focus of this review is on the controversial issues in the field of EPCs which are related to the lack of a unique EPC marker, identification challenges related to the paucity of EPCs in the circulation, and the important phenotypical and functional overlap between EPCs, haematopoietic cells and mature ECs. We also discuss our recent findings on the origin of endothelial outgrowth cells (EOCs), showing that this in vitro defined EC population does not originate from circulating CD133(+) cells or CD45(+) haematopoietic cells.
Equilibrium Ring-Closing Metathesis (2009)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTEquilibrium Ring-Closing MetathesisSebastien Monfette and Deryn E. Fogg*View Author Information Department of Chemistry and Centre for Catalysis Research & Innovation, University of Ottawa, 10 Marie Curie, Ottawa, Ontario, K1N 6N5, CanadaCite this: Chem. Rev. 2009, 109, 8, 3783–3816Publication Date (Web):June 19, 2009Publication History Received3 December 2008Published online19 June 2009Published inissue 12 August 2009https://pubs.acs.org/doi/10.1021/cr800541yhttps://doi.org/10.1021/cr800541yreview-articleACS PublicationsCopyright © 2009 American Chemical SocietyRequest reuse permissionsArticle Views12104Altmetric-Citations336LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Catalysts,Equilibrium,Hydrocarbons,Metathesis,Oligomers Get e-Alerts
Subcutaneous Insulin Absorption Explained by Insulin's Physicochemical Properties: Evidence From Absorption Studies of Soluble Human Insulin and Insulin Analogues in Humans (1991)

Association state is a major determinant of rates of absorption of insulin and insulin analogues. The lag phase and the subsequent increasing rate of subcutaneous soluble insulin absorption can be explained by the associated state of native insulin in pharmaceutical formulation and its progressive dissociation into smaller units during the absorption process.
Molecular Strategy for Detecting Metastatic Cancers with Use of Multiple Tumor-specific MAGE-A Genes (2001)

The uMAGE-A reverse transcription-PCR/ECL assay provides a practical and sensitive approach for detection of various metastatic cancers in tissues and blood.
Intracardiac Stimulation of Human Parasympathetic Nerve Fibers Induces Negative Dromotropic Effects: (1998)

Electrical stimulation of parasympathetic nerve fibers near the posteroseptal and anteroseptal areas could induce a negative dromotropic effect, and this effect was preserved after successful radiofrequency ablation of slow pathway and isthmus conduction.
A novel route for the preparation of narrow particle size distribution emulsions and microcapsules† (1999)

A process is described for making emulsions, and microcapsules derived from them, of known, narrow particle size distribution. This can greatly reduce problems of thermodynamic instability and degradation, and improve biological performance by permitting close control of the release rate of capsules. © 1999 Society of Chemical Industry
Assessment of Transient Changes in Corneal Hydration Using Confocal Raman Spectroscopy (2003)

The bovine eye experiments demonstrate that significant changes in corneal hydration are realized under different drying conditions and treatment methodologies. This study elucidates the nature of transient changes in corneal hydration in a bovine model and suggests the need for further study of the role of such variations in surgical outcome for excimer laser corneal refractive procedures.
Computer vision applied to super resolution (2003)

Super-resolution (SR) restoration aims to solve the following problem: given a set of observed images, estimate an image at a higher resolution than is present in any of the individual images. Where the application of this technique differs in computer vision from other fields is in the variety and severity of the registration transformation between the images. In particular this transformation is generally unknown, and a significant component of solving the SR problem in computer vision is the estimation of the transformation. The transformation may have a simple parametric form, or it may be scene dependent and have to be estimated for every point. In either case the transformation is estimated directly and automatically from the images. We describe the two key components that are necessary for successful SR restoration: the accurate alignment or registration of the LR images and the formulation of an SR estimator that uses a generative image model together with a prior model of the super-resolved image itself. As with many other problems in computer vision, these different aspects are tackled in a robust, statistical framework.
Biting Behavior, Aggression, and Seizures (2005)

Anatomic and electrophysiologic data in our patients suggest that a model of dual--temporal and frontal--dysfunction could account for the occurrence of ictal/postictal BB. Behavioral data suggest also that BB and related aggressive gestures can be considered as the emergence of instinctive behaviors with an adaptative significance of defense of the peripersonal space.
Overexpression of neurone glial‐related cell adhesion molecule is an independent predictor of poor prognosis in advanced colorectal cancer (2011)

A downstream target of the Wnt pathway, neurone glial-related cell adhesion molecule (Nr-CAM) has recently been implicated in human cancer development. However, its role in colorectal cancer (CRC) pathobiology and clinical relevance remains unknown. In this study, we examined the clinical significance of Nr-CAM protein expression in a retrospective series of 428 CRCs using immunohistochemistry and tissue microarrays. Cox proportional hazards regression was used to calculate hazard ratios (HR) of mortality according to various clinicopathological features and molecular markers. All CRC samples were immunoreactive for Nr-CAM protein expression, compared to 10 ⁄ 245 (4%) matched normal tissue (P < 0.0001). Of 428 CRC samples, 97 (23%) showed Nr-CAM overexpression, which was significantly associated with nodal (P = 0.012) and distant (P = 0.039) metastasis, but not with extent of local invasion or tumor size. Additionally, Nr-CAM overexpression was associated with vascular invasion (P = 0.0029), p53 expression (P = 0.036), and peritoneal metastasis at diagnosis (P = 0.013). In a multivariate model adjusted for other clinicopathological predictors of survival, Nr-CAM overexpression correlated with a significant increase in disease-specific (HR 1.66; 95% confidence interval 1.11-2.47; P = 0.014) and overall mortality (HR 1.57; 95% confidence interval 1.07-2.30; P = 0.023) in advanced but not early stage disease. Notably, 5-fluorouracil-based chemotherapy conferred significant survival benefit to patients with tumors negative for Nr-CAM overexpression but not to those with Nr-CAM overexpressed tumors. In conclusion, Nr-CAM protein expression is upregulated in CRC tissues. Nr-CAM overexpression is an independent marker of poor prognosis among advanced CRC patients, and is a possible predictive marker for non-beneficence to 5-fluorouracil- based chemotherapy.
Survival of the Myeloid Progenitor Cell Line FDC-P1 is Prolonged by Interferon-γ or Interleukin-4 (1992)

Continuous proliferation of the immortalized myeloid progenitor cell line FDC-P1 depends on stimulation with either interleukin-3 (IL-3) or granulocyte-macrophage colony stimulating factor (GM-CSF). Two other cytokines, interferon-γ (IFN-γ) and IL-4, were found to prolong FDC-P1 survival for several days. Surviving cells incorporated [3H]thymidine and a minority completed up to 3 cell divisions before dying. This transient proliferative response was a direct effect of IFN-γ and IL-4 since these cytokines did not induce production of detectable IL-3 or GM-CSF and the response was unaffected by cell concentration. IL-6, a constitutive product of FDC-P1 cells whose secretion was increased by IL-3, GM-CSF and IL-4 but not by IFN-γ, was not responsible for the proliferative response. FDC-P1 lines that constirutively expressed the cell cycle-associated oncogene myc or the survival-associated oncogene bcl-2 also responded only transiently to IFN-γ or IL-4, indicating that expression of these genes did not complement the signals delivered by IFN-γ or IL-4. By contrast, the protein kinase C activator phorbol 12-myristate 13-acetate (PMA) prolonged survival of FDC-P1 cells on its own and potentiated the response to IFN-γ or IL-4, although the combination of stimuli did not support long-term growth. It is concluded that IFN-γ and IL-4 trigger only some of the signalling events that lead to mitogenesis; these events are complemented by stimulation with PMA but additional signals are required for sustained proliferation.
Folding Proteome of Yarrowia lipolytica Targeting with Uracil Permease Mutants (2010)

The acquisition of the correct folding of membrane proteins is a crucial process that involves several steps from the recognition of nascent protein, its targeting to the endoplasmic reticulum membrane, its insertion, and its sorting to its final destination. Yarrowia lipolytica is a hemiascomycetous dimorphic yeast and an alternative eukaryotic yeast model with an efficient secretion pathway. To better understand the quality control of membrane proteins, we constructed a model system based on the uracil permease. Mutated forms of the permease were stabilized and retained in the cell and made the strains resistant to the 5-fluorouracil drug. To identify proteins involved in the quality control, we separated proteins extracted in nondenaturing conditions on blue native gels to keep proteins associated in complexes. Some gel fragments where the model protein was immunodetected were subjected to mass spectrometry analysis. The proteins identified gave a picture of the folding proteome, from the translocation across the endoplasmic reticulum membrane, the folding of the proteins, to the vesicle transport to Golgi or the degradation via the proteasome. For example, EMC complex, Gsf2p or Yet3p, chaperone membrane proteins of the endoplasmic reticulum were identified in the Y. lipolytica native proteome.
Generation and functional analyses for base-substitution mutants of the adenovirus 2 major late promoter (1984)

The function of guanosine residues surrounding the TATA box of the adenovirus 2 major late promoter (MLP) in promoting efficient transcription initiation and in selecting a specific transcription start site in vitro has been examined. Multiple and single base substitutions (G----A) were generated in this region (from -63 to +25 relative to the cap site, +1) of the MLP. The promoter activities of the wild type and 21 mutants were assayed in an in vitro transcription system using whole cell extract (WCE) prepared from HeLa cells. The results suggest that the strings of G residues immediately adjacent to the TATA box are not required for full promoter activity in vitro. These G residues also appear not to be involved in the selection of a specific transcription start site by RNA polymerase II in vitro, since the identical cap site was used by wild-type and mutated MLP's. However, two G residues (-55 and -57) were identified as part of an upstream promoter element: a G----A transition at either -55 or -57 resulted in a 2.6 fold reduction in promoter activity. However, neither single nor double G----A transitions at -62 and -63 had an effect on promoter activity.
An exon skipping screen identifies antitumor drugs that are potent modulators of pre-mRNA splicing, suggesting new therapeutic applications (2020)

Agents that modulate pre-mRNA splicing are of interest in multiple therapeutic areas, including cancer. We report our recent screening results with the application of a cell-based Triple Exon Skipping Luciferase Reporter (TESLR) using a library that is composed of FDA approved drugs, clinical compounds, and mechanistically characterized tool compounds. Confirmatory assays showed that three clinical antitumor therapeutic candidates (milciclib, PF-3758309 and PF-562271) are potent splicing modulators and that these drugs are, in fact, nanomolar inhibitors of multiple kinases involved in the regulation the spliceosome. We also report the identification of new SF3B1 antagonists (sudemycinol C and E) and show that these antagonists can be used to develop a displacement assay for SF3B1 small molecule ligands. These results further support the broad potential for the development of agents that target the spliceosome for the treatment of cancer and other diseases, as well as new avenues for the discovery of new chemotherapeutic agents for a range of diseases.
Fiber Bragg grating differential strain sensor (2004)

In the paper a parametric, fiber optic strain sensor with Bragg gratings, in a differential scheme is described. The possibility of compensating the effect of temperature on the accuracy of the strain processing is shown.
Lysine acetylation can generate highly charged enzymes with increased resistance toward irreversible inactivation (2008)

This paper reports that the acetylation of lysine epsilon-NH3(+) groups of alpha-amylase--one of the most important hydrolytic enzymes used in industry--produces highly negatively charged variants that are enzymatically active, thermostable, and more resistant than the wild-type enzyme to irreversible inactivation on exposure to denaturing conditions (e.g., 1 h at 90 degrees C in solutions containing 100-mM sodium dodecyl sulfate). Acetylation also protected the enzyme against irreversible inactivation by the neutral surfactant TRITON X-100 (polyethylene glycol p-(1,1,3,3-tetramethylbutyl)phenyl ether), but not by the cationic surfactant, dodecyltrimethylammonium bromide (DTAB). The increased resistance of acetylated alpha-amylase toward inactivation is attributed to the increased net negative charge of alpha-amylase that resulted from the acetylation of lysine ammonium groups (lysine epsilon-NH3(+) --> epsilon-NHCOCH3). Increases in the net negative charge of proteins can decrease the rate of unfolding by anionic surfactants, and can also decrease the rate of protein aggregation. The acetylation of lysine represents a simple, inexpensive method for stabilizing bacterial alpha-amylase against irreversible inactivation in the presence of the anionic and neutral surfactants that are commonly used in industrial applications.
A New Heterostructure for 2DEG System with a Si Atomic-Planar-Doped AlAs-GaAs-AlAs Quantum Well Structure Grown by MBE (1985)

Using Si atomic-planar-doping technology, a new heterostructure, called “Planar-Doped Quantum Well (PD-QW) Heterostructure”, which has a single Si-doped Ga plane with sheet concentration of 0.6–4 ×10 12 cm -2 in an AlAs-GaAs-AlAs quantum well structure as a 2DEG supplying layer was developed. It exhibited 2DEG mobility of 8.7 ×10 4 cm 2 /Vs at 77 K with sheet concentration of 1.0 ×10 12 cm -2 , in addition to much reduced persistent photoconductivity at low temperatures. The sheet resistivity of the 2DEG layer in this material (about 70 Ω/\Box at 77 K) is the lowest observed so far in selectively doped single-interface heterostructures.
A photochemical approach to the synthesis of (±)-biotin (1981)

A synthesis of (±)-biotin 1 based on a photochemical cycloaddition reaction of l,3-diacetylimidazolin-2-one 3 is presented. Oxidative rearrangement of the cyclobutanol 5 has provided the key intermediate 6 having the necessary stereochemical features for elaboration of n-pentyl biotin 2.
A novel injectable scaffold for cartilage tissue engineering using adipose‐derived adult stem cells (2007)

Articular cartilage has a limited self-regenerative capacity. Thus, treatment of cartilage lesions is a major challenge. Tissue engineering using a variety of biomaterials is a promising solution to the problem of cartilage damage. In this in vitro study, we investigated the effect of the presence of cartilage-tissue chondroitin-sulfate (CS) in a fibrin scaffold on the differentiation of adipose-derived adult stem cells (ADAS cells) into chondrocytes. Isolated rabbit ADAS cells were cultured in fibrin matrices with and without CS for up to 14 days. ADAS cells differentiated into chondrocytes in both matrices, but cell proliferation, glycoaminoglycans content, and type II collagen expression were significantly higher in the fibrin-CS matrices than those in the fibrin matrices alone. Histological examination and scanning electronic microscopy revealed the fibrin-CS matrices exceeded in inducing differentiation of ADAS cells into chondrocytes in terms of tissue morphological characteristics. We concluded that the fibrin-CS matrices mimicking native cartilage extracellular matrix could act as a three-dimensional scaffold for cartilage tissue engineering and have the potential for promoting ADAS cells differentiation into chondrocytes.
Implementing a Protected Zone in a Reconfigurable Processor for Isolated Execution of Cryptographic Algorithms (2009)

We design and realize a protected zone inside a reconfigurable and extensible embedded RISC processor for isolated execution of cryptographic algorithms. The protected zone is a collection of processor subsystems such as functional units optimized for high-speed execution of integer operations, a small amount of local memory, and general- and special-purpose registers. We outline the principles for secure software implementation of cryptographic algorithms in a processor equipped with the protected zone. We also demonstrate the efficiency and effectiveness of the protected zone by implementing major cryptographic algorithms, namely RSA, elliptic curve cryptography, and AES in the protected zone. In terms of time efficiency, software implementations of these three cryptographic algorithms outperform equivalent software implementations on similar processors reported in the literature. The protected zone is designed in such a modular fashion that it can easily be integrated into any RISC processor; its area overhead is considerably moderate in the sense that it can be used in vast majority of embedded processors. The protected zone can also provide the necessary support to implement TPM functionality within the boundary of a processor.
Investigations with a model surface wave transmission line (1957)

This paper summarizes the results of experimental studies conducted with a scale model of a surface wave transmission line (swt line) for long-distance transmission. The purpose of the experiments was to determine the effect of supports, bends, and sags on the transmission loss of such a line. Also, the coupling between parallel identical lines has been measured and compared with the theory. Formulas and other information are given which may be applied to the design of a swt line for long-distance transmission.
Application of the bacteriophage Mu-driven system for the integration/amplification of target genes in the chromosomes of engineered Gram-negative bacteria—mini review (2011)

The advantages of phage Mu transposition-based systems for the chromosomal editing of plasmid-less strains are reviewed. The cis and trans requirements for Mu phage-mediated transposition, which include the L/R ends of the Mu DNA, the transposition factors MuA and MuB, and the cis/trans functioning of the E element as an enhancer, are presented. Mini-Mu(LR)/(LER) units are Mu derivatives that lack most of the Mu genes but contain the L/R ends or a properly arranged E element in cis to the L/R ends. The dual-component system, which consists of an integrative plasmid with a mini-Mu and an easily eliminated helper plasmid encoding inducible transposition factors, is described in detail as a tool for the integration/amplification of recombinant DNAs. This chromosomal editing method is based on replicative transposition through the formation of a cointegrate that can be resolved in a recombination-dependent manner. (E-plus)- or (E-minus)-helpers that differ in the presence of the trans-acting E element are used to achieve the proper mini-Mu transposition intensity. The systems that have been developed for the construction of stably maintained mini-Mu multi-integrant strains of Escherichia coli and Methylophilus methylotrophus are described. A novel integration/amplification/fixation strategy is proposed for consecutive independent replicative transpositions of different mini-Mu(LER) units with "excisable" E elements in methylotrophic cells.
Antiproliferative Potencies of Interferons on Melanoma Cell Lines and Xenografts: Higher Efficacy of Interferon β (1992)

The finding that melanoma cell lines differ intrinsically in their sensitivity to IFNs may explain differences in clinical response. Our results suggest that IFN-beta may be the most effective IFN in the treatment of melanoma, although confirmation will require clinical trials involving large numbers of patients.
Multi-Antenna Communications over Rapidly Fading Channels Estimation and Space-Time Coding (2002)

This 3-year project dealt with development and testing of efficient algorithms for modeling and (blind) estimation of time-varying communication channels, and the resulting (self-recovering) antenna receivers and equalizers in rapidly fading mobile battlefield scenarios. Highlights of recent results include space-time coded transmit- and receive-diversity to combat noise, oscillator drifts, and Doppler effects; optimal wedding of beamforming with space-time coding; space-time-frequency coding for use over frequency-selective channels; space-time-Doppler coding for time-selective channels; and optimal training over doubly-selective channels.