gebhart/patrepeval huggingface dataset:
Modification of Aldose Reductase by S-Nitrosoglutathione (1997)

Kinetic and structural changes in recombinant human aldose reductase (AR) due to modification by S-nitrosoglutathione (GSNO) were investigated. Incubation of the enzyme with 10-50 microM GSNO led to a time- and concentration-dependent inactivation of the enzyme, with a second-order rate constant of 0.087 +/- 0.009 M-1 min-1. However, upon exhaustive modification, 30-40% of the enzyme activity was retained. The non-inactivated enzyme displayed a 2-3-fold change in Km for NADPH and Km fordl-glyceraldehyde, whereas the Km for the lipid peroxidation product, 4-hydroxy-2-trans nonenal (HNE), was comparable to that of the untreated enzyme. The residual activity of the enzyme after GSNO treatment was less sensitive to inhibition by the active site inhibitor sorbinil or to activation by sulfate. Significantly higher catalytic activity was retained when the enzyme was modified in the presence of NADPH, suggesting relatively low reactivity of the E-NADPH complex with GSNO. The modification site was identified using site-directed mutants in which each of the solvent-exposed cysteines of the enzyme was replaced individually by serine. The mutant C298S was insensitive to GSNO, whereas the sensitivity of the mutants C303S and C80S was comparable to that of the wild-type enzyme. Electrospray ionization mass spectroscopy of the GSNO-modified enzyme revealed a major modified species (70% of the protein) with a molecular mass that was 306 Da higher than that of the untreated enzyme, which is consistent with the addition of a single glutathione molecule to the enzyme. The remaining 30% of the protein displayed a molecular mass that was not significantly different from that of the native enzyme. No nitrosated forms of the enzyme were observed. These results suggest that inactivation of AR by GSNO is due to the selective formation of a single mixed disulfide between glutathione and Cys-298 located at the NADP(H)-binding site of the enzyme.
Effect of cellular changes and onset of humoral immunity on the replication of porcine reproductive and respiratory syndrome virus in the lungs of pigs (2000)

Twenty-two 4- to 5-week-old gnotobiotic pigs were intranasally inoculated with 10(6.0) TCID(50) of porcine reproductive and respiratory syndrome virus (PRRSV) (Lelystad) and euthanized at different time intervals post-inoculation (p.i.). Bronchoalveolar lavage (BAL) cell populations were characterized, together with the pattern of virus replication and appearance of antibodies in the lungs. Total BAL cell numbers increased from 140x10(6) at 5 days p.i. to 948x10(6) at 25 days p.i. and remained at high levels until the end of the experiment. The number of monocytes/macrophages, as identified by monoclonal antibodies 74-22-15 and 41D3, increased two- to fivefold between 9 and 52 days p.i. with a maximum at 25 days p.i. Flow cytometry showed that the population of differentiated macrophages was reduced between 9 and 20 days p.i. and that between the same time interval, both 74-22-15-positive and 41D3-negative cells, presumably monocytes, and 74-22-15- and 41D3-double negative cells, presumably non-phagocytes, entered the alveolar spaces. Virus replication was highest at 7 to 9 days p.i., decreased slowly thereafter and was detected until 40 days p.i. Anti-PRRSV antibodies were detected starting at 9 days p.i. but neutralizing antibodies were only demonstrated in one pig euthanized at 35 days and another at 52 days p.i. The decrease of virus replication in the lungs from 9 days p.i. can be attributed to (i) shortage of susceptible differentiated macrophages, (ii) lack of susceptibility of the newly infiltrated monocytes and (iii) appearance of anti-PRRSV antibodies in the lungs. Neutralizing antibodies may contribute to the clearance of PRRSV from the lungs.
Characterization of Tumor-Suppressive Function of SOX6 in Human Esophageal Squamous Cell Carcinoma (2010)

We provided the first evidence that SOX6 is a novel tumor-suppressor gene in ESCC development and is a potential prognostic marker in ESCC.
An Efficient Coarse-to-Fine Indexing Technique for Fast Text Retrieval in Historical Documents (2012)

In this paper, we present a fast text retrieval system to index and browse degraded historical documents. The indexing and retrieval strategy is designed in a two level, coarse-to-fine approach, to increase the speed of the retrieval process. During the indexing step, the text parts in the images are encoded into sequences of primitives, obtained from two different codebooks: a coarse one corresponding to connected components and a fine one corresponding to glyph primitives. A glyph consists of a single character or a part of a character according to the shape complexity. During the querying step, the coarse and the fine signature are generated from the query image using both codebooks. Then, a bi-level approximate string matching algorithm is applied to find similar words, using coarse approach first, and then the fine approach if necessary, by exploiting predetermined hypothetical locations. An experimental evaluation on datasets of real life document images, gathered from historical books of different scripts, demonstrated the speed improvement and good accuracy in presence of degradation.
Potential agents for removal of actinides from waste solutions (1996)

The uptake of Th(IV) from nitric acid and hydrochloric acid solutions by chelating ion exchange resins containing catechol, 1,2- hydroxypyridinone (1,2-HOPO) and 3,4-hydroxypyridinone (3,4-HOPO) functional groups, has been investigated. These polystyrene based materials show excellent kinetics for uptake of Th(IV) and have a high loading capacity. Liquid/liquid extractants have also been synthesized by addition of lipophilic side chains to the chelating groups (1,2-HOPO; 3,4-HOPO; 3,2-HOPO; catecholamide; terephthalamide). The initial evaluation of the extraction properties has been carried out.
Does IgE Bind to and Activate Eosinophils from Patients with Allergy? (1999)

Human eosinophils have been reported to express both the mRNA and protein for the high affinity IgE receptor (FcepsilonRI); it is speculated that this receptor plays a role in eosinophil mediator release in allergic diseases. However, questions still remain. How much of the FcepsilonRI protein is actually expressed on the cell surface of the eosinophil? If they are present, are these IgE receptors associated with effector functions of eosinophils? To address these issues, we studied blood eosinophils from patients with ragweed hay fever. A high level of low affinity IgG receptor (FcgammaRII, CD32), but no expression of FcepsilonRI, was detectable on the eosinophil surface by standard FACS analysis. However, after in vitro sensitization with biotinylated chimeric IgE (cIgE), cell-bound cIgE was detected by PE-conjugated streptavidin. This cIgE binding was partially inhibited by anti-FcepsilonRI mAb, suggesting that eosinophils do express minimal amounts of FcepsilonRI detectable only by a sensitive method. Indeed, FACS analysis of whole blood showed that eosinophils express approximately 0.5% of the FcepsilonRI that basophils express. When stimulated with human IgE or anti-human IgE, these eosinophils did not exert effector functions; there was neither production of leukotriene C4 or superoxide anion nor any detectable degranulation response. In contrast, eosinophils possessed membrane-bound human IgG and showed functional responses when stimulated with human IgG or anti-human IgG. Thus, IgG and/or cytokines, such as IL-5, appear to be more important for eosinophil activation in allergic diseases than IgE.
Bacterial Contamination of Blood Components (2005)

Blood for transfusion is a potential source of infection by a variety of known and unknown transmissible agents. Over the last 20 years, astounding reductions in the risk of viral infection via allogeneic blood have been achieved. As a result of this success, bacterial contamination of blood products has emerged as the greatest residual source of transfusion-transmitted disease. This paper summarizes the current status of detection, prevention, and elimination of bacteria in blood products for transfusion.
What is a missed dose? Implications for construct validity and patient adherence (2006)

The construct 'missed dose' is central to many assessments of medication adherence. However, few studies have investigated how patients or clinicians conceptualize missed doses or the extent of the concordance or discordance between clinicians and patients. To address this gap we conducted semi-structured interviews with 45 sero-positive African American adults taking HAART and 17 of their clinicians. Results reveal large variability in missed dose conceptions among both patients and physicians and significant differences between the two groups. Overall, patients reported a stricter definition of missed dose than did clinicians. Fifty-five percent of patients thought that a pill-taking delay of six hours beyond the prescribed dosing time constituted a missed dose, by comparison, only one physician agreed with this assessment. More than one-third of patients thought that the proper response to a missed dose would be to skip it altogether, but only about 12% of clinicians agreed. These findings have implications for the construct validity of self-report measures of adherence, for patient adherence behaviours based on missed dose conceptions and for patient-physician relationships and communication within the clinical environment.
20 ${\hbox{kA/cm}}^{2}$ Process Development for Superconducting Integrated Circuits With 80 GHz Clock Frequency (2007)

Results of the development of an advanced fabrication process for superconductor integrated circuits (ICs) with 20 kA/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> Nb/AlO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</sub> /Nb Josephson junctions is presented. The process has 4 niobium superconducting layers, one MoN <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</sub> resistor layer with 4.0 Ohm per square sheet resistance for the junction shunting and circuit biasing, and employs circular Josephson junctions with the minimum diameter of 1 mum; total 11 photolithography levels. The goal of this process development is the demonstration of the feasibility of 80 GHz clock speeds in superconducting ICs for digital signal processing (DSP) and high performance computing. Basic components of rapid single flux quantum (RSFQ) logic such as DC/SFQ, SFQ/DC converters, Josephson transmission lines (JTLs), and simple digital circuits such as T-flip-flops and 4-bit digital counters have been fabricated and tested. The T-flip-flops were shown to operate up to 400 GHz with the widest margin of operation of plusmn13% at 325 GHz. Digital testing results on the 4-bit counters as well as the junctions, resistors, and other process parameters are also presented. Prospects for yet higher speeds and very large scale integration are discussed.
IM-05 * MULTISPECIFIC CAR T CELLS FOR THE TREATMENT OF HIGH GRADE GLIOMA (2015)

BACKGROUND: High grade gliomas (HGG) carry a dismal prognosis. Our group has shown that patients' HER2-specific chimeric antigen receptor (CAR) T-cells kill autologous HGG, yet 40% of tumors recur in experimental animals. Recurrences are composed of HER2 negative escape variant cells that maintain positivity for two other glioma antigens, IL13Rα2 and EphA2. We hypothesized that co-targeting of these escape variants will enhance the anti-glioma activity of CAR T cells. OBJECTIVES: Objectives: The intent of this project is to develop a multi-specific T-cell product for the adoptive immunotherapy of HGG. DESIGN/METHOD: We generated and tested the effector functions of patients' T-cells co-expressing CARs for HER2, IL13Rα2 and EphA2 against autologous HGG cells derived from surgical excision samples. RESULTS: Primary patient HGG samples exhibited varied expression of the three target antigens, HER2, IL13Rα2 and EphA2. In order to render single patients' T cells tri-specific, we designed a transgene incorporating 3 encoding regions for HER2, IL13Rα2 and EphA2 CARs separated by 2A sequences in a single cassette driven by a CMV promoter. We used a retroviral system to stably integrate this transgene into the primary T cell genome. CAR-specific flow cytometry indicated proportionate stable expression of individual CAR molecules on the T cell surface. These T cells show distinct specificity for each of the three glioma antigens evidenced by activation, proliferation, and cytolytic function in immunoassays. Further, preliminary in vitro testing in glioma cell lines shows the ability to target populations of tumor cells that are not eliminated by unspecific products. CONCLUSION: The heterogeneity of antigenic expression in HGG and antigen escape justify targeting multiple glioma antigens simultaneously. We have successfully generated and tested a multi-specific T cell product for adoptive transfer that could offset antigen escape and exhibit an enhanced anti-glioma efficacy.
Biomimetic Collagen–Hydroxyapatite Composite Fabricated via a Novel Perfusion-Flow Mineralization Technique (2012)

Prevalent three-dimensional scaffolds for bone tissue engineering are mineralized collagen-hydroxyapatite (Col/HA) composites. Conventional mineralization techniques are either to coat collagen scaffold surfaces with minerals or to simply mix collagen and mineral nanoparticles together. These conventional in vitro collagen mineralization methods are different from the in vivo bone formation process and often result in scaffolds that are not suitable for bone tissue engineering. In this study, a unique perfusion-flow (i.e., dynamic) in conjunction with a previously described polymer-induced liquid-precursor (PILP) method was used to fabricate a porous Col/HA composite. The dynamic flow emulated the physiological extracellular fluid flow containing the mineralization ions, while the PILP method facilitated the deposition of the HA crystals within the collagen fibrils (i.e., intrafibrillar mineralization). By utilizing a dynamic PILP technique to mimic the in vivo bone formation process, the resultant Col/HA composite has a similar structure and compositions like human trabecular bone. A comparison of the dynamic and static mineralization methods revealed that the novel dynamic technique facilitates more efficient and homogenous mineral deposition throughout the Col/HA composite. The dynamic intrafibrillar mineralization method generated stiff Col/HA composites with excellent surface property for cell attachment and growth. The human mesenchymal stem cells cultured on the Col/HA composites quickly remodeled the scaffolds and resulted in constructs with an extensive cell-derived extracellular matrix network.
Cyanobacterial phycobilisomes: Selective dissociation monitored by fluorescence and circular dichroism (1980)

Phycobilisomes are supramolecular assemblies of phycobiliproteins responsible for photosynthetic light collection in red algae and cyanobacteria. They can be selectively dissociated by reduction of temperature and buffer concentration. Phycobilisomes isolated from Fremyella diplosiphon transfer energy collected by C-phycoerythrin and C-phycocyanin to allophycocyanin. The energy transfer to allophycocyanin is nearly abolished at 2 degrees C, as indicated by a blue shift in fluorescence emission, and is accompanied by a decrease in the circular dichroism in the region of allophycocyanin absorbance. Further dissociation of the phycobilisomes can be attained by reduction of buffer concentration and holding at 2 degrees C. Energy transfer to C-phycocyanin is nearly abolished, and decreases occur in the circular dichroism in the region of C-phycocyanin and C-phycoerythrin absorbance. Complete dissociation of the phycobilisomes at low buffer concentration and 2 degrees C requires extended time. Energy transfer to C-phycocyanin is further reduced and the circular dichroism maximum of C-phycoerythrin at 575 nm is lost. Circular dichroism provides information on the hexamer-monomer transitions of the phycobiliproteins, whereas fluorescence is indicative of hexamer-hexamer interactions. We consider that hydrophobic interactions are fundamental to the maintenance of the structure and function of phycobilisomes.
Optimizing the beam pattern of a forward-viewing ring-annular ultrasound array for intravascular imaging (2002)

Intravascular ultrasound (IVUS) imaging systems using circumferential arrays mounted on cardiac catheter tips fire beams orthogonal to the principal axis of the catheter. The system produces high resolution cross-sectional images but must be guided by conventional angioscopy. A real-time forward-viewing array, integrated into the same catheter, could greatly reduce radiation exposure by decreasing angiographic guidance. Unfortunately, the mounting requirement of a catheter guide wire prohibits a full-disk imaging aperture. Given only an annulus of array elements, prior theoretical investigations have only considered a circular ring of point transceivers and focusing strategies using all elements in the highly dense array, both impractical assumptions. In this paper, we consider a practical array geometry and signal processing architecture for a forward-viewing IVUS system. Our specific design uses a total of 210 transceiver firings with synthetic reconstruction for a given 3-D image frame. Simulation results demonstrate this design can achieve side-lobes under -40 dB for on-axis situations and under -30 dB for steering to the edge of a 80 degrees cone.
Network layer mobility: an architecture and survey (1996)

We explore various network layer concepts that play a crucial role in the design of mobile networking systems. We show that mobility is essentially an address translation problem and is best resolved at the network layer. We describe services that must be supported at the network layer to carry out the task of address translation. Using these service primitives as building blocks, we describe a network-layer architecture which enables smooth integration of mobile end systems within the existing Internet. A summary of some of the key mobile IP proposals is presented, and it is shown that each proposal can be viewed as a special case of the architecture outlined in this article.
PHARMACOLOGY AND THERAPEUTICS ETRETINATE THERAPY FOR PSORIASIS AND OTHER KE RATINIZING DISORDERS: A 10‐YEAR RETROSPECTIVE STUDY IN SINGAPORE (1993)

Etretinate is effective for treating psoriasis and other keratinizing disorders. Combination therapy is preferred in chronic plaque psoriasis. Adverse effects are common, but mild and tolerable.
Non-destructive on-chip cell sorting system with real-time microscopic image processing (2004)

Studying cell functions for cellomics studies often requires the use of purified individual cells from mixtures of various kinds of cells. We have developed a new non-destructive on-chip cell sorting system for single cell based cultivation, by exploiting the advantage of microfluidics and electrostatic force. The system consists of the following two parts: a cell sorting chip made of poly-dimethylsiloxane (PDMS) on a 0.2-mm-thick glass slide, and an image analysis system with a phase-contrast/fluorescence microscope. The unique features of our system include (i) identification of a target from sample cells is achieved by comparison of the 0.2-microm-resolution phase-contrast and fluorescence images of cells in the microchannel every 1/30 s; (ii) non-destructive sorting of target cells in a laminar flow by application of electrostatic repulsion force for removing unrequited cells from the one laminar flow to the other; (iii) the use of agar gel for electrodes in order to minimize the effect on cells by electrochemical reactions of electrodes, and (iv) pre-filter, which was fabricated within the channel for removal of dust contained in a sample solution from tissue extracts. The sorting chip is capable of continuous operation and we have purified more than ten thousand cells for cultivation without damaging them. Our design has proved to be very efficient and suitable for the routine use in cell purification experiments.
The oxidative cleavability of protein cross-linking reagents containing organoselenium bridges (1990)

The intensive use of cleavable cross-linking reagents to study macromolecular biological interactions has shown a demand for optimizing these reagents in such a way that the involved macromolecules remain intact. The present work focuses on the development of selenium linkers that are cleavable by mild oxidation. The efficiency of cross-linking and subsequent cross-linker cleavage with a new series of such homo- or heterobifunctional cross-linking reagents have been tested in a simple model system, consisting of albumin and cytochrome c. Resultant, or residual, covalent complex formation is examined by SDS-polyacrylamide gel electrophoresis. From this work it can be concluded that diallyl selenides are readily cleaved by mild oxidation, whereas dialkyl selenides and benzyl alkyl selenides can only be cleaved when the alkyl part of the selenide has an electron-withdrawing group next to the beta-carbon from selenium.
The Synthesis and Incorporation in Oligonucleotides of a Thymidine Dimer Containing an Internucleoside Phosphinate Linkage (1995)

A phosphinic acid protecting group strategy has been applied to the synthesis of a dinucleotide analogue containing a phosphinate as replacement for the phosphate in a thymidine-thymidine dimer. The dinucleotide modification was readily incorporated into deoxy-nucleic acid via phosphoramidate chemistry. The biophysical profile, with respect to binding to the RNA complement, has been assessed as both a protected ethyl ester and as the free phosphinate.
Introduction (1959)

This bibliography brings up to 1959 the one published by Stillman and Dunlop. In general, the same basis of selection has been followed in this compilation; but in addition to Analytical Chemistry and The Analyst, Analytica Chimica Acta has been covered, because of its connection with the International Union of Pure and Applied Chemistry. The references are not necessarily limited to the use of photoelectric spectrophotometers, as other means of detection are used. A small number of papers deal with ultraviolet measurements, as reagents are being found which make work in this region feasible. Thus far, similar application of reagents to make inorganic systems measurable in the infrared region apparently has found little use. A few references concern substances not in the ionic condition.