gebhart/patrepeval huggingface dataset:
Applications of the piezoelectric crystal detector in analytical chemistry (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTApplications of the piezoelectric crystal detector in analytical chemistryJ. Hlavay and G. G. GuilbaultCite this: Anal. Chem. 1977, 49, 13, 1890–1898Publication Date (Print):November 1, 1977Publication History Published online1 May 2002Published inissue 1 November 1977https://pubs.acs.org/doi/10.1021/ac50021a007https://doi.org/10.1021/ac50021a007research-articleACS PublicationsRequest reuse permissionsArticle Views591Altmetric-Citations168LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
dSarm/Sarm1 Is Required for Activation of an Injury-Induced Axon Death Pathway (2012)

Axonal and synaptic degeneration is a hallmark of peripheral neuropathy, brain injury, and neurodegenerative disease. Axonal degeneration has been proposed to be mediated by an active autodestruction program, akin to apoptotic cell death; however, loss-of-function mutations capable of potently blocking axon self-destruction have not been described. Here, we show that loss of the Drosophila Toll receptor adaptor dSarm (sterile α/Armadillo/Toll-Interleukin receptor homology domain protein) cell-autonomously suppresses Wallerian degeneration for weeks after axotomy. Severed mouse Sarm1 null axons exhibit remarkable long-term survival both in vivo and in vitro, indicating that Sarm1 prodegenerative signaling is conserved in mammals. Our results provide direct evidence that axons actively promote their own destruction after injury and identify dSarm/Sarm1 as a member of an ancient axon death signaling pathway.
Synthesis of a cyclic analog of oxidized glutathione by an intersite reaction in a swollen polymer network (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of a cyclic analog of oxidized glutathione by an intersite reaction in a swollen polymer networkKuldeep K. Bhargava, Virender K. Sarin, Nguyen Le Trang, Anthony Cerami, and R. B. MerrifieldCite this: J. Am. Chem. Soc. 1983, 105, 10, 3247–3251Publication Date (Print):May 1, 1983Publication History Published online1 May 2002Published inissue 1 May 1983https://pubs.acs.org/doi/10.1021/ja00348a047https://doi.org/10.1021/ja00348a047research-articleACS PublicationsRequest reuse permissionsArticle Views234Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
In VivoObservations on Testosterone and Estradiol-17β Protein Binding in Women (1969)

The protein binding of radioactive testosterone and estradiol was studied following in vivo injection of the steroids. Serum samples from pregnant and nonpregnant women were partitioned sequentially by continuous paper electrophoresis, Sephadex column filtration and polyacrylamide gel electrophoresis. Non-albumin protein binding of each steroid was found to be increased during pregnancy. The protein binding patterns of testosterone and estradiol differed with respect to the proportion bound to albumin and their more specific binding protein (s). Testosterone-binding protein (TBP) and estradiol-binding protein (EBP) could not be separated in these studies. TBP-EBP had the properties of an α2-globulin on polyacrylamide gel electrophoresis.
Ig A EGFR antibodies mediate tumour killing in vivo (2013)

Currently all approved anti-cancer therapeutic monoclonal antibodies (mAbs) are of the IgG isotype, which rely on Fcgamma receptors (FcγRs) to recruit cellular effector functions. In vitro studies showed that targeting of FcαRI (CD89) by bispecific antibodies (bsAbs) or recombinant IgA resulted in more effective elimination of tumour cells by myeloid effector cells than targeting of FcγR. Here we studied the in vivo anti-tumour activity of IgA EGFR antibodies generated using the variable sequences of the chimeric EGFR antibody cetuximab. Using FcαRI transgenic mice, we demonstrated significant in vivo anti-tumour activity of IgA2 EGFR against A431 cells in peritoneal and lung xenograft models, as well as against B16F10-EGFR cells in a lung metastasis model in immunocompetent mice. IgA2 EGFR was more effective than cetuximab in a short-term syngeneic peritoneal model using EGFR-transfected Ba/F3 target cells. The in vivo cytotoxic activity of IgA2 EGFR was mediated by macrophages and was significantly decreased in the absence of FcαRI. These results support the potential of targeting FcαRI for effective antibody therapy of cancer.
Quantifying how location and dose of botulinum toxin injections affect muscle paralysis (1993)

Despite the widespread use of botulinum toxin to treat muscle dystonias, no method exists to quantify muscle paralysis in either human or nonhuman models. In this study we examined how the location, dose, and volume of botulinum injection affects paralysis in the rat tibialis anterior muscle. Paralysis was quantified by electrically stimulating the nerve to the tibialis anterior and then staining sections of the muscle for glycogen. The areas of glycogen-containing fibers represented regions of botulinum action. The results showed that the most important injection technique is to inject botulinum directly into the motor endplate region of a muscle. Injections only 0.5 cm from the motor endplate resulted in a 50% decrease in paralysis. Increases in dose increased paralysis, however, some of that increase was simply due to the increased volume of injection. Thus, delivering toxin in small volumes near the MEP band of a muscle should produce the most effective paralysis.
Activation of natural killer T cells by liposomal glycosphingolipids increases the efficacy of liposomal doxorubicin against DMBA-induced tumors in mice (VAC10P.968) (2014)

Abstract Natural Killer T (NKT) cells show potent antitumor activity upon activation with their specific ligands. Glycosphingolipids (GSLs) isolated from Sphingomonas paucimobilis were incorporated into DPPC-liposomes to prepare GSL-incorporated liposomes (GSL-liposomes). Treatment of tumor-bearing mice with a combination of GSL-liposomes and doxorubicin liposomes resulted into slowing down of the progression of tumors and increase in the survival of mice. Splenocytes obtained from mice treated with GSL-liposomes produced higher levels of IFN-γ and IL-12 compared to mice that did not receive GSL-liposomes. Mice pretreated with GSL-liposomes showed low frequency of tumors after DMBA exposure. These findings suggest that co-administration of NKT cells activating GSL-liposomes and anti-cancer drug loaded liposomes may be proved a better option for the treatment of cancer.
A Calorimetric Study of the Mechanism and Thermodynamics of the Lithium Hydride-Water Reaction at Elevated Temperatures (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA Calorimetric Study of the Mechanism and Thermodynamics of the Lithium Hydride-Water Reaction at Elevated TemperaturesJonathan Phillips, Michael C. Bradford, and Martin KlancharCite this: Energy Fuels 1995, 9, 4, 569–573Publication Date (Print):July 1, 1995Publication History Published online1 May 2002Published inissue 1 July 1995https://pubs.acs.org/doi/10.1021/ef00052a001https://doi.org/10.1021/ef00052a001research-articleACS PublicationsRequest reuse permissionsArticle Views143Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Platelet Serotonergic System in Depression and Following Sertraline Treatment (1988)

This preliminary study examined the effect of 8 weeks' treatment with the serotonin (5HT) uptake inhibitor, sertraline, on the platelet serotonergic system in depression. Patient pre-treatment platelet 5HT uptake rates and 5HT-mediated platelet aggregation responses were significantly reduced compared to the control values. Binding of the tritiated 5HT2 receptor antagonist, 3H-ketanserin, to platelet membranes from the patient group was increased above control levels. All the patients in the study had recovered from the depressive episode, assessed using the Hamilton depression rating scale, following 8 weeks' sertraline treatment. Eight weeks' sertraline treatment also resulted in a normalization of the biochemical parameters examined. Therefore, we conclude that sertraline is an effective antidepressant and these results confirm previous reports of abnormal platelet serotonin transport and aggregation response as putative markers of the depressed state.
Low Modulus Biomimetic Microgel Particles with High Loading of Hemoglobin (2012)

We synthesized extremely deformable red blood cell-like microgel particles and loaded them with bovine hemoglobin (Hb) to potentiate oxygen transport. With similar shape and size as red blood cells (RBCs), the particles were fabricated using the PRINT (particle replication in nonwetting templates) technique. Low cross-linking of the hydrogel resulted in very low mesh density for these particles, allowing passive diffusion of hemoglobin throughout the particles. Hb was secured in the particles through covalent conjugation of the lysine groups of Hb to carboxyl groups in the particles via EDC/NHS coupling. Confocal microscopy of particles bound to fluorescent dye-labeled Hb confirmed the uniform distribution of Hb throughout the particle interior, as opposed to the surface conjugation only. High loading ratios, up to 5 times the amount of Hb to polymer by weight, were obtained without a significant effect on particle stability and shape, though particle diameter decreased slightly with Hb conjugation. Analysis of the protein by circular dichroism (CD) spectroscopy showed that the secondary structure of Hb was unperturbed by conjugation to the particles. Methemoglobin in the particles could be maintained at a low level and the loaded Hb could still bind oxygen, as studied by UV-vis spectroscopy. Hb-loaded particles with moderate loading ratios demonstrated excellent deformability in microfluidic devices, easily deforming to pass through restricted pores half as wide as the diameter of the particles. The suspension of concentrated particles with a Hb concentration of 5.2 g/dL showed comparable viscosity to that of mouse blood, and the particles remained intact even after being sheared at a constant high rate (1000 1/s) for 10 min. Armed with the ability to control size, shape, deformability, and loading of Hb into RBC mimics, we will discuss the implications for artificial blood.
Antibody responses in buffalos immunized with Clostridium perfringens beta and epsilon toxoids (2001)

The antibody responses to toxoids were measured to investigate whether Clostridium perfringens beta and epsilon toxoids induced protective humoral immune responses in buffalos. Total of 24 buffalos were divided into 4 groups (n = 6), beta toxoid, epsilon toxoid, combination and control groups. These buffalo groups were administered each of the designated toxoids. Immunizations in the beta and epsilon toxoid groups induced strong antibody responses. The neutralizing antibody titres from the beta and epsilon toxoid groups were equally log101.2 on day 21 after inoculation whereas there was no antibody titre detected from the control group. A statistically significant (P < 0.01) increase in antibody titre was observed from day 0 to day 14 and 21 after inoculation. The antibody production did not vary significantly due to day of inoculation and toxoid interactions.
Monoclonal antibodies recognize different parts of Z-DNA. (1982)

Brominated poly(dG-dC) . poly(dG-dC), which forms a stable Z-DNA helix under physiological salt conditions, was used as an antigen to produce monoclonal antibodies from mice. The anti-Z-DNA antibodies were specific for the Z conformation as was shown by direct binding studies and a competitive solid phase assay. Competition could only be seen by brominated poly(dG-dC) . poly(dG-dC) under low and high salt conditions and unmodified poly(dG-dC) . poly(dG-dC) under high salt conditions. No binding could be seen to a number of other polynucleotides, including native and denatured DNA. Among the anti-Z-DNA monoclonal antibodies, examples differed in specificity: binding of one monoclonal antibody was blocked by methylation of cytosine; whereas the other antibody reacted equally well with the unmodified polymer or methyl cytosine-containing polymer in high ionic strength. The two antibodies are specific for different localized features of Z-DNA structure.
pl50/95, Third member of the LFA‐1/CR3 polypeptide family identified by anti‐Leu M5 monoclonal antibody (1985)

Monoclonal antibody (mAb) anti-Leu M5 reacts with a two-chain molecule composed of a 150-kDa alpha subunit noncovalently associated with a 95-kDa beta subunit and probably is specific for an epitope on the 150-kDa alpha chain. This p150/95 antigen is the third member of a family of polypeptides sharing a common 95-kDa beta chain, which includes the lymphocyte function-associated antigen LFA-1 (p177/95) and complement receptor CR3 (Mo1/MAC-1/OKM1; p165/95) antigens. Sequential immunoprecipitation with anti-p95 beta chain mAb specifically removed the antigens detected by anti-LFA-1, anti-CR3 and anti-Leu M5 mAb. Certain patients with recurrent bacterial infections are genetically deficient in expression of the LFA-1 and Mo1 antigens, and have impaired granulocyte function. Granulocytes from a patient with this disease also failed to react with anti-Leu M5. Stimulation of normal granulocytes with f-Met-Leu-Phe, C5a-desArg, or calcium ionophore resulted in increased expression of Mo1 and Leu M5 antigens on the cell surface, but did not significantly increase expression of LFA-1 antigen. In functional assays, anti-Leu M5 did not inhibit T cell-mediated or natural killer cell-mediated cytotoxicity. In addition, anti-Leu M5 neither inhibited the binding of complement-coated particles to CR1 or CR3 nor did it affect the binding of EC3dg to neutrophils (CR4). These studies clearly indicate that the p150/95 antigen recognized by the anti-Leu M5 antibody is a structurally distinct member of the LFA-1/CR3 family.
Biological Properties of Structurally Related α-Helical Cationic Antimicrobial Peptides (1999)

A series of alpha-helical cationic antimicrobial peptide variants with small amino acid changes was designed. Alterations in the charge, hydrophobicity, or length of the variant peptides did not improve the antimicrobial activity, and there was no statistically significant correlation between any of these factors and the MIC for Pseudomonas aeruginosa, Escherichia coli, or Salmonella typhimurium. Individual peptides demonstrated synergy with conventional antibiotics against antibiotic-resistant strains of P. aeruginosa. The peptides varied considerably in the ability to bind E. coli O111:B4 lipopolysaccharide (LPS), and this correlated significantly with their antimicrobial activity and ability to block LPS-stimulated tumor necrosis factor and interleukin-6 production. In general, the peptides studied here demonstrated a broad range of activities, including antimicrobial, antiendotoxin, and enhancer activities.
High‐resolution chromosome arm 5p array CGH analysis of small cell lung carcinoma cell lines (2004)

Genomic amplification of regions on chromosome arm 5p has been observed frequently in small cell lung cancer (SCLC), implying the presence of multiple oncogenes on this arm. Although conventional comparative genomic hybridization (CGH) detects gross chromosomal copy number changes, gene discovery requires a higher-resolution approach in order to identify regions of alteration precisely. To identify candidate genes on this chromosome arm, we developed a high-resolution, 10-clone-per-megabase bacterial artificial chromosome CGH array for 5p and examined a panel of 15 SCLC cell lines. Utilization of this CGH array has allowed the fine-mapping of breakpoints to regions as small as 200 kb in a single experiment. In addition to reporting our observations of aberrations at the well-characterized SKP2 and TERT loci, we describe the identification of microdeletions that have escaped detection by conventional screens and the identification TRIO and ANKH as novel putative oncogenes.
The peptide product of a 5' leader cistron in the beta 2 adrenergic receptor mRNA inhibits receptor synthesis. (1994)

The 5' leader region of mammalian beta 2 adrenergic receptor messenger RNAs (mRNA) have a short open reading frame (sORF) preceding the receptor cistron. Mutational inactivation of the sORF start codon increased beta 2 receptor expression and translation 1.9-fold from beta 2 receptor genes transfected into COS-7 cells. sORF inactivation also increased receptor synthesis 2.4-fold in a cell-free expression system that synthesizes functional beta 2 receptor in vitro. Translational initiation at the sORF was demonstrated both in vitro and in transfected COS-7 cells using an epitope-tagged fusion protein. Using the fusion protein as a reporter for initiation at the sORF shows that 5' leader mutations which increase translation of the sORF decrease receptor translation. Mutation analysis of the 5' leader region and peptide coding sequences suggests the peptide itself inhibits beta 2 receptor expression. Consistent with this hypothesis, a synthetic peptide corresponding to the peptide encoded by the beta 2 receptor sORF potently inhibits translation in vitro. Our results suggest that a nonoverlapping cistron in the beta 2 receptor mRNA 5' leader region is translated and the resulting peptide inhibits receptor translation.
Predicting Pharmaceutical Incompatibilities of Parenteral Medications (1968)

On the basis of data on the use of intravenous additives obtained from a survey of patient charts at a medical center hospital, the authors review the potential reactions of the penicillins (penicillin G, methicillin, phenethicillin and ampidllin), the tetracyclines, B complex vitamins, vitamin C, hydrocortisone and aminophylline. The influence of Ae acidic or basic character of the additives on their reactivity is emphasized. Using available data from chemicd incompatibility studies, a method of estimating the reaction rate of some additives is shown by calculating the time required for 10 percent of the drug to react. The authors emphasize the deficiencies and inconsistencies of published compatibility charts, particularly with respect to chemical incompatibilities, and urge hospital pharmacists to become better informed in this area.
Software Clustering based on Omnipresent Object Detection (2005)

The detection of omnipresent objects can be an important aid to the process of understanding a large software system. As a result, various detection techniques have been presented in the literature. However, these techniques do not take the subsystem structure into account when deciding whether an object is omnipresent or not. In this paper, we present a new set of detection methods for omnipresent objects that maintain that an object needs to be connected to a large number of subsystems before it is deemed omnipresent. We compare this novel approach to existing ones. We also introduce a framework that can improve the effectiveness of existing software clustering algorithms by combining them with an omnipresent object detection method. Experiments with two large software systems demonstrate the usefulness of this framework.
Plasticity of astroglia: Evidence supporting process elongation by “stretch” (1988)

The structural plasticity of cerebral astroglia was investigated in vivo by implantation experiments. Immunocytochemical markers for glia filament protein were used to identify the astrocytes. First it was established that implanted nitrocellulose filters provided a substrate for astrocytes from different brain regions of young rats. Astrocytes attached to the filter and projected fine processes into it. Longer implantation times increased the density and length of glial processes within filter spaces. Astrocytes that penetrated the filters implanted in the tectum exhibited more processes than those in the cortex, suggesting regional differences of astrocyte distributions. Second it was observed that astrocytes that attached to the filter formed elongated processes when they were tethered within an expanding matrix. This was shown by implanting the nitrocellulose filter together with PC12 cells that continued to grow. The implantation of neither PC12 cells without filters nor nitrocellulose filters alone induced the formation of elongated astroglia with parallel aligned processes, resembling radial glia. Such glial forms only occurred in the filter/PC12 cell cografts. This indicates that processes of astrocytes adherent to nitrocellulose filters could be stretched in response to expansion of the surrounding tissue.
Emergence of epidemic dengue/dengue hemorrhagic fever as a public health problem in the Americas. (1993)

The incidence of dengue and dengue hemorrhagic fever has increased dramatically in the past 15 years in most urban centers of the tropics. Coincident with this increase has been the emergence of epidemic dengue hemorrhagic fever in the American region. The current changing disease pattern in the Americas is very similar to that which occurred in southeast Asia 30 years ago. The similarities in the evolution of severe disease in the two regions and the possible reasons for the changing disease pattern are discussed.