gebhart/patrepeval huggingface dataset:
Molecular mechanisms of resistance to Rituximab and pharmacologic strategies for its circumvention (2009)

The introduction of Rituximab has greatly improved therapeutic options for patients with B-cell non-Hodgkin lymphoma (B-NHL). However, a substantial fraction of patients with aggressive B-NHL fails first-line therapy, and most patients with relapsing indolent B-NHL eventually acquire Rituximab resistance. Molecular understanding of the underlying mechanisms facilitates the development of pharmacologic strategies to overcome resistance. Rituximab exerts its activity on CD20-expressing B-cells by indirect and direct effector mechanisms. Indirect mechanisms are complement-dependent cytotoxicity (CDC), and antibody-dependent cell-mediated cytotoxicity (ADCC). Direct activities, such as growth inhibition, induction of apoptosis and chemosensitisation, have been reported, but are less defined. Moreover, the relative contribution of CDC, ADCC and direct mechanisms to the activity of Rituximab in vivo is unclear. Down-regulation of CD20 and expression of complement inhibitors have been described as escape mechanisms in B-NHL. Recent reports suggest that deregulated phosphoinositide-3-kinase (PI3K)/Akt, mitogen-activated kinases (MAPK) and nuclear-factor kappaB (NF-kappaB), as well as up-regulation of anti-apoptotic proteins may determine the efficacy of Rituximab to kill B-NHL cells in vitro and in vivo. The latter signalling pathways are attractive targets for pharmacologic modulation of resistance to Rituximab. With the advent of new inhibitors and antibodies, rationally designed clinical trials addressing Rituximab resistance are feasible.
Solubilization and reconstitution of the membrane‐bound carotenogenic enzymes from daffodil chromoplasts (1985)

The membrane-bound carotenogenic enzymes of daffodil (Narcissus pseudonarcissus) chromoplast membranes, i.e. dehydrogenation, cis-trans isomerization and cyclization activities, were solubilized in an enzymatically inactive form using the zwitterionic detergent 3-[(3-cholamidopropyl)-dimethylamino]-1-propanesulfonate (Chaps). Full enzymatic activities were regained upon reconstitution of the solubilized proteins into liposomes. These preparations converted radiolabelled cis-phytoene into beta-carotene in a high yield. The reconstituted enzymatic sequence behaved as a tight 'assembly line'. In the enzymatic sequence of the reconstituted system the cis-trans isomerization reaction occurred on the stage of cis-phytofluene after a dehydrogenation of cis-phytoene.
Large-Area Domain Alignment in Block Copolymer Thin Films Using Electric Fields (1998)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTLarge-Area Domain Alignment in Block Copolymer Thin Films Using Electric FieldsP. Mansky, J. DeRouchey, T. P. Russell, J. Mays, M. Pitsikalis, T. Morkved, and H. JaegerView Author Information Polymer Science and Engineering Department, Silvio O. Conte Center for Polymer Research, University of Massachusetts, Amherst, Massachusetts 01003 Department of Chemistry, University of Alabama at Birmingham, Birmingham, Alabama 35294-1240 James Franck Institute and Department of Physics, University of Chicago, Chicago, Illinois 60037 Cite this: Macromolecules 1998, 31, 13, 4399–4401Publication Date (Web):June 9, 1998Publication History Received26 February 1998Revised4 May 1998Published online9 June 1998Published inissue 1 June 1998https://pubs.acs.org/doi/10.1021/ma980299uhttps://doi.org/10.1021/ma980299urapid-communicationACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views997Altmetric-Citations150LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Copolymers,Electric fields,Electrodes,Scattering,Thin films Get e-Alerts
SilkTouch: A New Technology for Skin Resurfacing in Aesthetic Surgery (1995)

We describe layer-by-layer char-free facial skin resurfacing at very low CO2 laser power levels with a miniature "SilkTouch" microprocessor-controlled optomechanical flashscanner. This device provides excellent ablation depth control with minimal thermal damage to the dermis. Indications for the laser in aesthetic surgery include perioral, lips, and periorbital wrinkles, among others.
Multinuclear NMR Study of the Effect of Acid Concentration on Ion Transport in Phosphoric Acid Doped Poly(benzimidazole) Membranes (2012)

(1)H and (31)P NMR spectra, line widths, spin-lattice relaxation times (T(1)), and (1)H self-diffusion coefficients (D) were determined for two distinct poly(benzimidazole) (PBI) proton exchange membranes (PEM), para-PBI and dihydroxy-PBI (2OH-PBI), both incorporating varying concentrations of phosphoric acid. The study was performed over the temperature range of 20-180 °C, for phosphoric acid concentrations of 30, 50, and 70 wt %. Of the two samples, less mobility was indicated for the 2OH-PBI compared with the para-PBI at all acid concentrations. It was also observed that increasing the acid content resulted in an increase in the temperature at which the T(1) minimum or plateau occurred. (31)P spectra reveal the presence of pyrophosphates and in the case of the 50 and 70 wt % para-PBI samples higher oligomers such as tripolyphosphates. (1)H D data showed the 30 wt % para-PBI having almost identical values as the 70 wt % 2OH-PBI over the entire temperature range. In general, stronger short- and long-range interactions were observed in the 2OH-PBI matrix, yielding reduced translational proton transport compared to that of para-PBI. While these stronger interactions hinder translational proton diffusion, they could enhance proton transport by the Grotthuss or structure diffusion mechanism, the more favorable transport mechanism. Activation energies obtained from the (1)H D data supports a proton-hopping mechanism, with possible assistance from fast exchange between phosphate groups.
Low-Dose Inhaled Corticosteroids and the Prevention of Death from Asthma (2000)

The regular use of low-dose inhaled corticosteroids is associated with a decreased risk of death from asthma.
Low-Loss Plasmonic Metamaterials (2011)

New materials are being developed that meet the requirements for nanoscale photonics.
Clastogen‐induced micronuclei in peripheral blood erythrocytes: The basis of an improved micronucleus test (1980)

Abstract Micronuclei induced in bone marrow erythroblasts by clastogenic chemicals are easily detected in peripheral blood. In mice treated with nitrogen mustard, 7,12 dimethylbenz(a)anthracene, or cyclophosphamide, the peak incidence of micronucleated polychromatic erythrocytes in peripheral blood was at least as great as the maximum incidence in bone marrow. In each case the peak incidence in blood occurred on the day following the peak incidence observed in bone marrow. Thus, for general genetic screening purposes, monitoring micronuclei in peripheral blood rather than in bone marrow smears provides at least equal sensitivity, offers greater simplicity in sample preparation and scoring, permits multiple sampling of treated animals, and may also facilitate automated scoring and human cytogenetic monitoring.
Composition of A‐150 tissue‐equivalent plastic (1977)

In recent years, the use of tissue-equivalent materials has become quite common in fast-neutron dosimetry, with the A-150 plastic developed by Shonka et al. probably the most popular. Information on this specific plastic is scantily reported in the literature and as a consequence a preponderance of authors unknowingly reference an article by Shonka describing an early version of a tissue substitute plastic but having a different elemental composition than the present A-150 formulation. We have reviewed the results of 21 chemical analyses which have occurred over a time span of four years on a total of 14 samples of A-150 plastic and based on these data and the formulation of the plastic, have arrived at a suggested composition for A-150 tissue-equivalent plastic. The ambiguities of water absorption by nylon, one of the components of the plastic, and the uncertainty this reflects in the composition of the plastic were evaluated.
Probing the structure of the warfarin‐binding site on human serum albumin using site‐directed mutagenesis (2002)

The binding of warfarin to the following human serum albumin (HSA) mutants was examined: K195M, K199M, F211V, W214L, R218M, R222M, H242V, and R257M. Warfarin bound to human serum albumin (HSA) exhibits an intrinsic fluorescence that is approximately 10-fold greater than the corresponding signal for warfarin in aqueous solution. This property of the warfarin/HSA complex has been widely used to determine the dissociation constant for the interaction. In the present study, such a technique was used to show that specific substitutions in subdomain 2A altered the affinity of HSA for warfarin. The fluorescence of warfarin/mutant HSA complexes varied widely from the fluorescence of the warfarin/wild-type HSA complex at pH = 7.4, suggesting changes in the structure of the complex resulting from specific substitutions. The fluorescence of the warfarin/wild-type HSA complex increases about twofold as the pH is increased from 6.0 to 9.0 due to the neutral-to-base (N-B) transition, a conformational change that occurs in HSA as a function of pH. Changes in the fluorescence of warfarin/mutant HSA complexes as a function of pH suggests novel behavior for most HSA species examined. For the HSA mutants F211V and H242V, the midpoint of the N-B transition shifts from a wild-type pH of 7.8 to a pH value of 7.1-7.2.
The relevance of a hypoxic tumour microenvironment in prostate cancer (2009)

Research into the hypoxic tumour microenvironment is accelerating and the reversal of hypoxia is increasingly being suggested as a mechanism for improving cancer treatment. Recent studies have suggested that hypoxia is also a feature in prostate cancer and is associated with a poor prognosis. Hypoxia has been shown to cause radio-resistance and hence hamper one of the major treatments for prostate cancer. However, unlike other solid tumours, such as cervical and head-and-neck cancer, there are inconsistencies and unanswered questions about the relevance of hypoxia in prostate cancer. This review outlines the role of low-oxygen conditions in prostate cancer and the areas where further studies are required.
Isolation and characterization of homogeneous acetate kinase from Salmonella typhimurium and Escherichia coli. (1986)

Acetate kinase from Salmonella typhimurium and Escherichia coli was purified to electrophoretic homogeneity. The amino acid compositions of both proteins were similar, and the apparent molecular weights were the same, about 40,000 for the putative monomers. The native proteins gave higher molecular weights, suggesting that the enzymes may be oligomers, perhaps with two polypeptide subunits. Steady-state kinetic studies were performed with the enzymes isolated from both organisms and the kinetic constants were determined. The Km values were 0.07 and 7 mM for ATP and acetate, respectively. In contrast to earlier studies using less pure preparations, the homogeneous enzymes from both strains were active only with acetate but not with propionate or butyrate. The enzyme activity was cold-labile, and the length of reactivation time in the presence of Mg X ATP and acetate was dependent on protein concentration, suggesting that the monomer may not be catalytically active. The enzyme was phosphorylated with [gamma-32P]ATP and the phosphoprotein was isolated. Phosphoacetate kinase was capable of transferring the phosphate group to either ADP or acetate. The accompanying paper (Fox, D. K., Meadow, N. D., and Roseman, S. (1986) J. Biol. Chem. 261, 13498-13503) shows that the phosphoryl group of phosphoacetate kinase can also be reversibly transferred to Enzyme I of the phosphoenolpyruvate:glycose phosphotransferase system.
Nucleic Acid Modulation of Gene Expression: Approaches for Nucleic Acid Therapeutics Against Cancer (2005)

Most cancers are characterized by abnormal gene expression, which is thought to contribute to the pathogenesis and maintenance of the malignant phenotype; abnormal proliferation, maturation, and apoptosis. Silencing such genes would appear to be a rational approach to the therapy of cancer, and some preliminary clinical studies support this concept. Of the strategies available, the anti-mRNA gene silencing approach has attracted much attention and is the focus of this review. This strategy includes three types of agents: (1) single-stranded antisense oligonucleotides; (2) catalytically active oligonucleotides, such as ribozymes, and DNAzymes that possess inherent RNA cleaving activity; and (3) small interfering RNA (siRNA) molecules that induce RNA interference (RNAi). Among these agents, antisense oligonucleotides, especially phosphorothioate (PS) oligonucleotides, have been the most frequently used in clinical trials. In this article, we provide an overview of anti-mRNA gene silencing agents and their development for use as cancer therapeutics.
Increased axon regeneration in astrocytes grown in the presence of proteoglycan synthesis inhibitors (1995)

ABSTRACT We have recently reported that the critical difference between astrocytic cell lines that are good or poor promoters of axon growth lies in the extracellular matrix. We demonstrated that much of this difference between matrix produced by permissive and non-permissive cell lines could be ascribed to one or more dermatan/keratan sulphate proteoglycans and that these proteoglycans are able to block the neurite-promoting effect of laminin. These proteoglycans are also produced by cultures of primary astrocytes. In the present study, we have demonstrated that treatment of both astrocytic cell lines and primary astro-cytes with inhibitors of proteoglycan synthesis, β-D-xylosides and sodium chlorate, can strongly influence the axon growth promoting properties of both matrix and whole cells. Dorsal root ganglia grown on matrix or in con-ditioned medium from cultures treated with β-D-xylosides or sodium chlorate had twice as many axons and the axons grew to twice the length as in control cultures. Following treatment of Neu7 cells with proteoglycan synthesis inhibitors there was also a significant reduction in the ability of Neu7 conditioned medium to block the neurite-promoting effect of laminin. Dorsal root ganglia grown on Neu7 cells treated with sodium chlorate extended 2 to 3 times the number of axons for approximately 300 mm longer distance than on control cultures. Treatment of Neu7 cells with β-D-xylosides, however, did not make the cells less inhibitory to axon growth. We have also examined the effects of proteoglycan synthesis inhibitors on three-dimensional primary astrocyte cultures, which closely mimic the in vivo effects of astrocytes on axon growth. Here again, dorsal root ganglia extended many more axons for a longer distance than in control cultures. We conclude from these results that interfering with astrocyte proteo-glycan synthesis makes them better able to support axon regeneration.
Speaker-independent name retrieval from spellings using a database of 50000 names (1991)

A system that recognizes names spelled with pauses between letters using high quality speech is described. The system uses neural network classifiers to locate and classify letters, then searches a database of names to find the best match to the letter scores. The directory name retrieval system was evaluated on 1020 names provided by 34 speakers who were not used to train the system. Using a database of 50000 names, 972, or 95.3%, were correctly identified as the first choice. Of the remaining 48 names, all but 10 were in the top three choices. 99% of letters were correctly located, although speakers failed to pause completely about 10% of the time. Classification of individual spoken letters that were correctly located was 93%.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A multi-layer fuzzy logical model for emotional speech perception (2005)

Perceiving emotion from speech plays an important role in human communication. In the filed of computer science, previous works tried to build the relationship between categories of emotion in speech and acoustic features by statistics. But they do not provide ways and means of both perception and expression. According to an observation of a phenomenon how we perceive emotion from speech, a threelayer model is proposed in this paper. A new layer primitive features which is a linguistic form of adjectives for describing speech voice is added. The purpose of this study is building and proving the model by a two-phase approach. In this paper, we introduce to the model and describe the first phase of building of the model using a top-down method to build two relationships. Firstly, the building of the first relationship between the emotional speech and the primitive feature is described. Three experiments to select suitable primitive features and the application of fuzzy inference system to build the relationship are then described. Finally, the building of the second relationship between the primitive feature and the acoustic features are demonstrated. The resultant relationships show significance of the proposed three-layer model.
Effect of Adenovirus-mediated Nitric Oxide Synthase Gene Transfer on Vasospasm after Experimental Subarachnoid Hemorrhage (2000)

These results demonstrate that adenovirus vectors can be used to transfer genes to cells in the subarachnoid space of dogs. Enough NO can be produced in the absence of SAH to dilate the basilar artery. After SAH, however, NO plus a cofactor can dilate arteries in vitro, but not enough NO is generated in the subarachnoid space to prevent vasospasm, perhaps owing to the scavenging of NO by hemoglobin.
Specific fluorogenic substrates for neprilysin (neutral endopeptidase, EC 3.4.24.11) which are highly resistant to serine- and metalloproteases (1997)

Two intramolecularly quenched fluorogenic peptides containing o-aminobenzoyl (Abz) and ethylenediamine 2,4-dinitrophenyl (EDDnp) groups at amino- and carboxyl-terminal amino acid residues, Abz-DArg-Arg-Leu-EDDnp (Abz-DRRL-EDDnp) and Abz-DArg-Arg-Phe-EDDnp (Abz-DRRF-EDDnp), were selectively hydrolyzed by neutral endopeptidase (NEP, enkephalinase, neprilysin, EC 3.4.24.11) at the Arg-Leu and Arg-Phe bonds, respectively. The kinetic parameters for the NEP-catalyzed hydrolysis of Abz-DRRL-EDDnp and Abz-DRRF-EDDnp were K(m) = 2.8 microM, kcat = 5.3 min-1, kcat/K(m) = 2 min-1 microM-1 and K(m) = 5.0 microM, kcat = 7.0 min-1, kcat/K(m) = 1.4 min-1 microM-1, respectively. The high specificity of these substrates was demonstrated by their resistance to hydrolysis by metalloproteases [thermolysin (EC 3.4.24.2), angiotensin-converting enzyme (ACE; EC 3.4.24.15)], serineproteases [trypsin (EC 3.4.21.4), alpha-chymotrypsin (EC 3.4.21.1)] and proteases present in tissue homogenates from kidney, lung, brain and testis. The blocked amino- and carboxyl-terminal amino acids protected these substrates against the action of aminopeptidases, carboxypeptidases and ACE. Furthermore, DR amino acids ensured total protection of Abz-DRRL-EDDnp and Abz-DRRF-EDDnp against the action of thermolysin and trypsin. Leu-EDDnp and Phe-EDDnp were resistant to hydrolysis by alpha-chymotrypsin. The high specificity of these substrates suggests their use for specific NEP assays in crude enzyme preparations.
Air-entrainment characteristics in a plunging water jet system using rectangular nozzles with rounded ends (2004)

When a water jet impinges a pool of water at rest, air bubbles may be entrained and carried some way below the pool's free surface. This process is called air-entrainment or aeration by a plunging water jet. In this study, air-entrainment characteristics such as volumetric airflow rate, air-entrainment rate, and bubble penetration depth in a plunging jet system using inclined rectangular nozzles with rounded ends were studied depending on effective jet width at impact point. An empirical correlation for an inclined rectangular nozzle with rounded ends is presented to predict the air-entrainment rate as a function of effective jet expansion ratio. Water SA Vol.29(1) 2003: 35-38
Mesenchymal stem cells protect against obstruction-induced renal fibrosis by decreasing STAT3 activation and STAT3-dependent MMP-9 production (2016)

STAT3 is a transcription factor implicated in renal fibrotic injury, but the role of STAT3 in mesenchymal stem cell (MSC)-induced renoprotection during renal fibrosis remains unknown. We hypothesized that MSCs protect against obstruction-induced renal fibrosis by downregulating STAT3 activation and STAT3-induced matrix metalloproteinase-9 (MMP-9) expression. Male Sprague-Dawley rats underwent renal arterial injection of vehicle or MSCs (1 × 10<sup>6</sup>/rat) immediately before sham operation or induction of unilateral ureteral obstruction (UUO). The kidneys were harvested after 4 wk and analyzed for collagen I and III gene expression, collagen deposition (Masson's trichrome), fibronectin, α-smooth muscle actin, active STAT3 (p-STAT3), MMP-9, and tissue inhibitor of matrix metalloproteinases 1 (TIMP-1) expression. In a separate arm, the STAT3 inhibitor S3I-201 (10 mg/kg) vs. vehicle was administered to rats intraperitoneally just after induction of UUO and daily for 14 days thereafter. The kidneys were harvested after 2 wk and analyzed for p-STAT3 and MMP-9 expression, and collagen and fibronectin deposition. Renal obstruction induced a significant increase in collagen, fibronectin, α-SMA, p-STAT3, MMP-9, and TIMP-1 expression while exogenously administered MSCs significantly reduced these indicators of obstruction-induced renal fibrosis. STAT3 inhibition with S3I-201 significantly reduced obstruction-induced MMP-9 expression and tubulointerstitial fibrosis. These results demonstrate that MSCs protect against obstruction-induced renal fibrosis, in part, by decreasing STAT3 activation and STAT3-dependent MMP-9 production.
Expression of Mcm7 and Cdc6 in oral squamous cell carcinoma and precancerous lesions. (2009)

High expressions of Mcm7 and Cdc6 are correlated with the development and metastasis of OSCC and may become a molecular marker for the early diagnosis and prognosis prediction for OSCC.