gebhart/patrepeval huggingface dataset:
Millimeter-Wave Enhanced Local Area Systems: A High-Data-Rate Approach for Future Wireless Networks (2014)

Wireless data traffic is projected to skyrocket 10 000 fold within the next 20 years. To tackle this incredible increase in wireless data traffic, a first approach is to further improve spectrally efficient systems such as 4G LTE in bands below 6 GHz by using more advanced spectral efficiency techniques. However, the required substantial increase in system complexity along with fundamental limits on hardware implementation and channel conditions may limit the viability of this approach. Furthermore, the end result would be an extremely spectrally efficient system with little room for future improvement to meet the ever-growing wireless data usage. The second approach is to move up in frequency, into an unused nontraditional spectrum where enormous bandwidths are available, such as at millimeter wave (mmWave). The mmWave option enables the use of simple air interfaces since large bandwidths can be exploited (e.g., 2 GHz) to achieve high data rates rather than relying on highly complex techniques originally aimed at achieving a high spectral efficiency with smaller bandwidths. In addition, mmWave systems will easily evolve to even higher system capacities, because there will be plenty of margin to improve the spectral efficiency as data demands further increase. In this paper, a case is made for using mmWave for a fifth generation (5G) wireless system for ultradense networks by presenting an overview of enhanced local area (eLA) technology at mmWave with emphasis on 5G requirements, spectrum considerations, propagation and channel modeling, air-interface and multiantenna design, and network architecture solutions.
Novel Plasmid-Borne Gene qacJ Mediates Resistance to Quaternary Ammonium Compounds in Equine Staphylococcus aureus , Staphylococcus simulans , and Staphylococcus intermedius (2003)

We identified a novel plasmid-borne gene (designated qacJ) encoding resistance to quaternary ammonium compounds (QACs) in three staphylococcal species associated with chronic infections in four horses. qacJ was located on a 2,650-bp plasmid (designated pNVH01), a new member of the pC194 family of rolling-circle replication plasmids. The 107-amino-acid protein, QacJ, showed similarities to known proteins of the small multidrug resistance family: Smr/QacC (72.5%), QacG (82.6%), and QacH (73.4%). The benzalkonium chloride MIC for a qacJ-containing recombinant was higher than those for otherwise isogenic recombinants expressing Smr, QacG, or QacH. Molecular epidemiological analyses by pulsed-field gel electrophoresis suggested both the clonal spread of a qacJ-harboring Staphylococcus aureus strain and the horizontal transfer of pNVH01 within and between different equine staphylococcal species. The presence of pNVH01 of identical nucleotide sequence in different staphylococcal species suggests that recent transfer has occurred. In three of the horses, a skin preparation containing cetyltrimethylammonium bromide had been used extensively for several years; this might explain the selection of staphylococci harboring the novel QAC resistance gene.
Phage display of a catalytic antibody to optimize affinity for transition-state analog binding (1997)

Catalytic antibodies have shown great promise for catalyzing a tremendously diverse set of natural and unnatural chemical transformations. However, few catalytic antibodies have efficiencies that approach those of natural enzymes. In principle, random mutagenesis procedures such as phage display could be used to improve the catalytic activities of existing antibodies; however, these studies have been hampered by difficulties in the recombinant expression of antibodies. Here, we have grafted the antigen binding loops from a murine-derived catalytic antibody, 17E8, onto a human antibody framework in an effort to overcome difficulties associated with recombinant expression and phage display of this antibody. "Humanized" 17E8 retained similar catalytic and hapten binding properties as the murine antibody while levels of functional Fab displayed on phage were 200-fold higher than for a murine variable region/human constant region chimeric Fab. This construct was used to prepare combinatorial libraries. Affinity panning of these resulted in the selection of variants with 2- to 8-fold improvements in binding affinity for a phosphonate transition-state analog. Surprisingly, none of the affinity-matured variants was more catalytically active than the parent antibody and some were significantly less active. By contrast, a weaker binding variant was identified with 2-fold greater catalytic activity and incorporation of a single substitution (Tyr-100aH --> Asn) from this variant into the parent antibody led to a 5-fold increase in catalytic efficiency. Thus, phage display methods can be readily used to optimize binding of catalytic antibodies to transition-state analogs, and when used in conjunction with limited screening for catalysis can identify variants with higher catalytic efficiencies.
Sensor validation for power plants using adaptive backpropagation neural network (1990)

Signal validation and process monitoring problems in many cases require the prediction of one or more process variables in a system. The feasibility of using neural networks to characterize one variable as a function of other related variables is studied. The backpropagation network (BPN) is used to develop models of signals from both a commercial power plant and the Experimental Breeder Reactor-II (EBR-II). Several innovations are made in the algorithm, the most significant of which is the progressive adjustment of the sigmoidal threshold function and weight updating terms.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Radiological Comparison of Instrumented Posterior Lumbar Interbody Fusion With One or Two Closed-Box Plasmapore Coated Titanium Cages (2008)

In the long-term, instrumented PLIF with a single closed-box titanium cage shows similar radiologic results compared to fusion with 2 titanium cages. Implantation of a single titanium closed-box cage in an instrumented PLIF seems to be adequate in case of degenerative lumbar spinal disease.
Scalable kernel performance for internet servers under realistic loads (1998)

UNIX Internet servers with an event-driven architecture often perform poorly under real workloads, even if they perform well under laboratory benchmarking conditions. We investigated the poor performance of eventdriven servers. We found that the delays typical in widearea networks cause busy servers to manage a large number of simultaneous connections. We also observed that the select system call implementation in most UNIX kernels scales poorly with the number of connections being managed by a process. The UNIX algorithm for allocating file descriptors also scales poorly. These algorithmic problems lead directly to the poor performance of eventdriven servers. We implemented scalable versions of the select system call and the descriptor allocation algorithm. This led to an improvement of up to 58% in Web proxy and Web server throughput, and dramatically improved the scalability of the system. 1 Introduction Many Web servers and proxies are implemented as as single-threaded event-driv...
Specific induction of secreted proteins by transforming growth factor-beta and 12-O-tetradecanoylphorbol-13-acetate. Relationship with an inhibitor of plasminogen activator. (1987)

To examine the mechanisms by which transforming growth factors (TGFs) regulate the proliferation of eukaryotic cells, five cell lines, from different species and tissues, were treated with three agents that inhibit DNA synthesis and proliferation: BSC-1 cell-derived growth inhibitor (GI/TGF-beta), platelet-derived transforming growth factor-beta (TGF-beta), and 12-O-tetradecanoylphorbol-13-acetate. The cell lines tested were mink lung CCL 64 epithelial cells, Maloney sarcoma virus-transformed CCL 64.1, monkey kidney BSC-1 epithelial cells, human epidermoid A431 cells, and mouse embryo AKR-2B (clone 84A) cells. All cell lines responded to one or more of these agents by synthesizing and secreting a 48 to 51-kDa protein (IIP48). The TGF-beta s and 12-O-tetradecanoylphorbol-13-acetate had little or no effect on the incorporation of [35S] methionine into other secreted proteins or on the pattern of [35S]methionine-labeled intracellular proteins analyzed by one-dimensional, sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The maximum increase in induction of IIP48 varied from 2-fold to greater than 800-fold compared with the controls and occurred within 6 h of adding GI/TGF-beta to CCL 64 cells. Actinomycin D, alpha-amanitin, or 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole selectively decreased both the control and induced levels of IIP48 even after as little as 6 h of incubation. Thus, it appears that IIP48 mRNA turns over rapidly. Induction of IIP48 was dissociated from the inhibition of DNA synthesis by GI/TGF-beta. However, we found that epidermal growth factor and GI/TGF-beta act synergistically to increase the secreted level of IIP48. Others have shown that epidermal growth factor and TGF-beta act synergistically to stimulate growth of cells in agar. IIP48 from CCL 64, BSC-1, and AKR-2B cells is specifically immunoprecipitated by antibody to bovine plasminogen activator inhibitor. We found previously that TGF-beta also inhibits the production of major excreted protein, a thiol protease. It is proposed that TGF-beta is able to promote anchorage-independent growth of untransformed cells because of its ability to inhibit the production of secreted proteases and to increase the production of protease inhibitors.
Recovering the true targets of specific ligands by virtual screening of the protein data bank (2004)

The Protein Data Bank (PDB) has been processed to extract a screening protein library (sc-PDB) of 2148 entries. A knowledge-based detection algorithm has been applied to 18,000 PDB files to find regular expressions corresponding to either protein, ions, co-factors, solvent, or ligand atoms. The sc-PDB database comprises high-resolution X-ray structures of proteins for which (i) a well-defined active site exists, (ii) the bound-ligand is a small molecular weight molecule. The database has been screened by an inverse docking tool derived from the GOLD program to recover the known target of four unrelated ligands. Both the database and the inverse screening procedures are accurate enough to rank the true target of the four investigated ligands among the top 1% scorers, with 70-100 fold enrichment with respect to random screening. Applying the proposed screening procedure to a small-sized generic ligand was much less accurate suggesting that inverse screening shall be reserved to rather selective compounds.
A Critical Role for Antigen-Specific Th1 Cells in Acute Liver Injury in Mice (1999)

A novel liver injury model was established in mice by targeting of OVA-containing liposomes into the liver, followed by adoptive transfer of OVA-specific Th1 cells. Combined treatment of mice with OVA-containing liposomes and Th1 cell transfer caused an increase in serum transaminase activity that was paralleled with an elevation of serum IFN-gamma levels. In sharp contrast, OVA-specific Th2 cell transfer resulted in an increase of serum IL-4 levels, but did not induce liver injury. Neither NK, NK T, nor CD8+ T cells were required for the Th1-induced liver injury. The liver injury was blocked by anti-IFN-gamma mAb and anti-TNF-alpha mAb, but not by anti-Fas ligand mAb. The Fas/Fas ligand independency was also demonstrated using Fas-deficient lpr mice. These findings indicate that Th1 cells are the major effector cells in acute liver injury.
Isolation and characterization of cell membranes. (1989)

The steps outlined above should provide a framework for developing and evaluating a scheme for isolating a particular membrane system of interest. Further characterization often focuses on the specific research interests of an investigator. Most subsequent techniques will probably involve biochemical analyses of protein and lipid components and biophysical analyses of membrane properties. The techniques to be used, such as SDS gel electrophoresis, enzyme kinetics, receptor binding, protein purification, etc. are not unique to membrane systems and will not be described in detail. However, it is likely that the functional relevance and success of subsequent analyses will be aided if the starting membranes are as homogeneous and well-characterized as possible. Even if some of the fractionation steps are bypassed, such as might occur if a specific membrane protein is detergent-extracted, purified, and reconstituted directly from a crude homogenate, a full understanding of membrane protein function will probably require complementary studies on intact cells and isolated membranes.
The Importance of Being Expert (2015)

Crowdsourcing is a computational paradigm whose distinctive feature is the involvement of human workers in key steps of the computation. It is used successfully to address problems that would be hard or impossible to solve for machines. As we highlight in this work, the exclusive use of nonexpert individuals may prove ineffective in some cases, especially when the task at hand or the need for accurate solutions demand some degree of specialization to avoid excessive uncertainty and inconsistency in the answers. We address this limitation by proposing an approach that combines the wisdom of the crowd with the educated opinion of experts. We present a computational model for crowdsourcing that envisions two classes of workers with different expertise levels. One of its distinctive features is the adoption of the threshold error model, whose roots are in psychometrics and which we extend from previous theoretical work. Our computational model allows to evaluate the performance of crowdsourcing algorithms with respect to accuracy and cost. We use our model to develop and analyze an algorithm for approximating the best, in a broad sense, of a set of elements. The algorithm uses naïve and expert workers to find an element that is a constant-factor approximation to the best. We prove upper and lower bounds on the number of comparisons needed to solve this problem, showing that our algorithm uses expert and naïve workers optimally up to a constant factor. Finally, we evaluate our algorithm on real and synthetic datasets using the CrowdFlower crowdsourcing platform, showing that our approach is also effective in practice.
A cardioprotective role for the endothelial protein C receptor in lipopolysaccharide-induced endotoxemia in the mouse (2004)

A model of gram-negative lethal endotoxin shock, involving continuous peritoneal infusion of lipopolysaccharide (LPS), has been applied to wild-type (WT) mice and mice with a severe deficiency of endothelial protein C receptor (EPCR(delta/delta)). The survival of EPCR(delta/delta) mice was significantly diminished as compared to WT mice after administration of LPS via this route. Heart rates and central blood pressures also were significantly more depressed in EPCR(delta/delta) mice, indicating that the receptor-based protein C (PC) pathway functions in regulation of hemodynamic properties in the mouse. Further, heart muscle damage was more severe in EPCR(delta/delta) mice as compared to WT mice after endotoxin administration, as revealed by the more elevated plasma myoglobin levels in EPCR(delta/delta) mice and by microscopic examination of stained heart sections. Neutrophil infiltration was more pronounced in heart tissue of EPCR(delta/delta) mice, perhaps in response to the greatly increased expression level of the chemokine, MIP-2, which also significantly more up-regulated in the LPS-treated EPCR(delta/delta) mouse cohort. In conclusion, a severe deficiency of EPCR adversely affects survival of mice subjected to continuous infusion of endotoxin, via contributions of more responsive hemodynamic and cardiac alterations, thus suggesting that, among its other functions, the PC-based receptor system has a cardioprotective role after acute inflammatory challenge.
Controlling non-inertial cavitation microstreaming for applications in biomedical research (2012)

Within this paper non-inertial cavitation microstreaming is investigated as a method for applying a mechanical stress to biological cells in vitro. A microfluidic device is designed in which microstreaming generated around microbubbles adhered to the floor of the acoustic chamber can be targeted at cells. The repeatability and controllability of microstreaming are investigated by computing μPIV vector fields of microstreaming flows. The uniformity of the acoustic pressure field in the x-y plane of the acoustic chamber is characterized by measuring the prevalence of microstreaming throughout the device.
[The molecular basis of resistance to anti-influenza drugs]. (2006)

Influenza A viruses resistant to adamantanes(amantadine and rimantadine) have drastically increased in the last few years. Moreover, these adamantane-resistant viruses have acquired resistance without compromising viral pathogenicity and transmissibility. Viruses resistant to the other anti-influenza drugs, neuraminidase (NA) inhibitors, have also been shown to emerge at a rate higher than previously thought. In addition, several recent observations suggest that human-to-human transmission of variants resistant to NA inhibitors may have occurred, contrary to earlier predictions that such variants were much less likely to be transmitted. Although the prevalence of NA inhibitor resistants remains low, surveillance of drug-resistant influenza viruses in communities is essential. Here, we review antiviral resistance in influenza viruses and the molecular mechanisms of the acquisition of resistance to these drugs.
Protection of weanling hamsters from experimental infection with wild‐type parainfluenza virus type 3 (para 3) by cold‐adapted mutants of para 3 (1986)

Parainfluenza virus type 3 (para 3) was adapted to replicate at 20 degrees C, a nonpermissive temperature for wild-type (wt) para 3. Serial passage at 20 degrees C resulted in the generation of cold-adapted (ca) and temperature-sensitive (ts) mutants. These mutant viruses have been characterized both in vitro and in vivo [Belshe and Hissom (1982): Journal of Medical Virology 10:235-242; Crookshanks and Belshe (1984): Journal of Medical Virology 13:243-249]. We now report the evaluation of three mutants (clone 1150, passaged 12 times in the cold [cp12], clone 1146, passaged 18 times in the cold [cp18], and clone 1328, passaged 45 times in the cold [cp45]) for their ability to protect hamsters from infection by wild-type para 3. Ether-anesthetized male syrian hamsters were intranasally vaccinated with either wt para 3 (clone 127) or one of the ca para 3 mutants and on day 28 post-vaccination; each animal was intranasally challenged with 10(5.0) pfu of wt para 3. On days 1, 2, 3, and 4 post-challenge, 4 to 13 hamsters from each group were sacrificed, and the quantity of para 3 in the nasal turbinates and lungs was determined. Wt virus induced protection from challenge. cp12, cp18, and cp45 reduced the peak titer of wt replication in the lungs by greater than 100-fold, tenfold, and tenfold, respectively. The duration of virus replication was shortened also by intranasal vaccination with the mutants. These data give evidence of an inverse relationship between the degree of protection induced by vaccination with cold-adapted mutants and the number of passages of the virus in the cold.
Composition and arrangement of genes define the strength of IRES-driven translation in bicistronic mRNAs (2001)

In addition to the cap-dependent mechanism, eukaryotic initiation of translation can occur by a cap-independent mechanism which directs ribosomes to defined start codons enabled by internal ribosome entry site (IRES) elements. IRES elements from poliovirus and encephalomyocarditis virus are often used to construct bi- or oligocistronic expression vectors to co-express various genes from one mRNA. We found that while cap-dependent translation initiation from bicistronic mRNAs remains comparable to monocistronic expression, internal initiation mediated by these viral IRESs is often very inefficient. Expression of bicistronic expression vectors containing the hepatitis B virus core antigen (HBcAg) together with various cytokines in the second cistron of bicistronic mRNAs gave rise to very low levels of the tested cytokines. On the other hand, the HBcAg was well expressed when positioned in the second cistron. This suggests that the arrangement of cistrons in a bicistronic setting is crucial for IRES-dependent translation of the second cistron. A systematic examination of expression of reporter cistrons from bicistronic mRNAs with respect to position was carried out. Using the dual luciferase assay system we show that the composition of reading frames on a bicistronic mRNA and the order in which they are arranged define the strength of IRES-dependent translation. Although the cellular environment and the nature of the IRES element influence translation strength the dominant determinant is the nature and the arrangement of cistrons on the mRNA.
Indexing visual representations through the complexity map (2002)

In differential geometry curves are characterized as mappings from an interval to the plane. In topology curves are characterized as a Hausdorff space with certain countability properties. Neither of these definitions captures the role that curves play in vision, however, in which curves can denote simple objects (such as a straight line), or complicated objects (such as a jumble of string). The difference between these situations is in part a measure of their complexity, and in part a measure of their dimensionality. Note that the map defining such curves is unknown, as is the proper way to represent them. We propose a formal complexity theory of curves appropriate for computational vision in general, and for problems like separating straight lines from jumbles in particular. The theory is applied to the problem of perceptual grouping.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>