gebhart/patrepeval huggingface dataset:
E1AF/PEA3 Activates the Rho/Rho-Associated Kinase Pathway to Increase the Malignancy Potential of Non–Small-Cell Lung Cancer Cells (2005)

E1AF/PEA3, an Ets family transcription factor, is frequently overexpressed in non-small-cell lung cancers (NSCLCs). Overexpression of E1AF increases motility and invasion of VMRC-LCD and NCI-H226 NSCLC cells, which lack endogenous E1AF expression, and the effect is synergistically increased by hepatocyte growth factor (HGF). The small GTPase Rho/Rho-associated kinase (ROCK) pathway is also involved in motility and invasion. To determine the role of the Rho/ROCK pathway in malignant phenotypes induced by E1AF, we analyzed VMRC-LCD cells transfected with an E1AF expression vector (LCD-E1AF cells) or with empty vector (LCD-vector cells). LCD-E1AF cells had more GTP-bound (active) Rho than LCD-vector cells and Rho activation was synergistically increased by HGF. The Rho activation by E1AF and HGF was also shown in NCI-H226 cells. Phosphorylation of myosin light chain (MLC), a downstream effector of ROCK signaling, was higher in LCD-E1AF cells than in LCD-vector cells, especially under HGF treatment. A specific ROCK inhibitor, Y27632, strongly suppressed MLC phosphorylation, cell motility, and invasion. In nude mice implanted s.c. and intrapulmonarily, LCD-E1AF cells made more local tumors than LCD-vector cells (six of six versus one of seven mice and four of seven versus one of seven mice, respectively). Three of the four mice with lung tumors from LCD-E1AF cells had lymph node metastases whereas the mouse with LCD-vector tumors did not. LCD-E1AF tumors showed higher MLC phosphorylation than LCD-vector tumors. These results suggest that E1AF activates the Rho/ROCK pathway in an HGF-enhanced manner and its activation is important in E1AF-induced motility and invasion as well as tumorigenesis and metastasis in NSCLC cells.
Delayed Contrast-Enhanced MRI of the Coronary Artery Wall in Takayasu Arteritis (2012)

Our findings suggest that LGE of the coronary artery wall seems to be common in patients with TA and similarly pronounced as in CAD patients. The observed coronary LGE seems to be rather unspecific, and differentiation between coronary vessel wall fibrosis and inflammation still remains unclear.
Understanding wide-band MOS transistors (1990)

A quantitative understanding of MOS-transistor speed has been slow to emerge because of the absence of a commonly agreed-upon figure of merit for MOS-transistor speed and a lack of familiarity among designers with MOS-amplifier topologies. It is suggested that these problems can be addressed through the use of the unity-gain current frequency (f/sub T/) as a figure of merit for MOS transistors, the use of f/sub T/ in the prediction of amplifier bandwidth, and a wider familiarity among designers with practical examples of MOS wideband amplifiers. The use of f/sub T/ as a figure of merit is discussed, and the achievable amplifier bandwidths are determined. Increasing the f/sub T/ of an MOS device by making the g/sub m/ larger or the C/sub g/ smaller, or both, is discussed. Wideband CMOS amplifiers are considered.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Efficient heuristics for the minimum shortest path Steiner arborescence problem with applications to VLSI physical design (1997)

Given an undirected graph G = (K E) with positive edge weights (lengths) w : E + R+, a set of terminals (sinks) N C V, and a unique root node r E N, a shortest-path Ste%er arborescence (simply called an arborescence in the following) is a Steiner tree rooted at T spanning all terminals in N such that every source-to-sink path is a shortest path in G. Given a triple (G, N, T), the Minimum Shortest-Path Steiner Arborescence (MSPSA) problem seeks an arborescence with minimum weight. The MSPSA problem has various applications in the areas of VLSI physical design, multicast network communication, and supercomputer message routing; various cases have been studied in the literature. In this paper, we propose three efficient heuristics for the MSPSA problem and present applications to VLSI physical design. Experiments indicate that our algorithms generate near-optimal results and achieve speedups of several orders of magnitude over existing algorithms.
Multilevel Path Planning for Nonholonomic Robots Using Semiholonomic Subsystems (1998)

We present a new and complete multilevel approachfor solving path- planning problems for nonholonomic robots. At the first level, a path is found that disrespects (some of) the nonholonomic constraints. At each of the next levels, a new path is generated by transformation of the path generated at the previous level. The transformation is such that more nonholonomic constraints are respected than at the previous level. At the final level, all nonholonomic constraints are respected. We present two techniques for these transformations. The first, which we call the pick and link technique, repeatedly picks pieces from the given path, and tries to replace them by more feasible ones. The second technique restricts the free configuration space to a "tube" around the given path, and a road map that captures the free-space connectivity within this tube is constructed by the prob abilistic path planner. From this road map we retrieve a new, more feasible path. In the intermediate levels, we plan paths for what we refer to as semiholonomic subsystems. Such systems are obtained by taking real (physical) systems, and removing some of their nonholonomic constraints. In this paper, we apply the scheme to carlike robots pulling trail ers, that is, tractor-trailer robots. In this case, the real system is the tractor-trailer robot, and the ignored constraints in the semiholo nomic subsystems are the kinematic ones on the trailers. These are the constraints of rolling without slipping, on the trailer's wheels. Experimental results are given that illustrate the time efficiency of the resulting planner. In particular, we show that using the multilevel scheme leads to significantly better performance (in computation time and path shape) than direct transformations to feasible paths.
Three-Dimensional Scaffolds for Tissue Engineering Applications: Role of Porosity and Pore Size (2013)

Tissue engineering applications commonly encompass the use of three-dimensional (3D) scaffolds to provide a suitable microenvironment for the incorporation of cells or growth factors to regenerate damaged tissues or organs. These scaffolds serve to mimic the actual in vivo microenvironment where cells interact and behave according to the mechanical cues obtained from the surrounding 3D environment. Hence, the material properties of the scaffolds are vital in determining cellular response and fate. These 3D scaffolds are generally highly porous with interconnected pore networks to facilitate nutrient and oxygen diffusion and waste removal. This review focuses on the various fabrication techniques (e.g., conventional and rapid prototyping methods) that have been employed to fabricate 3D scaffolds of different pore sizes and porosity. The different pore size and porosity measurement methods will also be discussed. Scaffolds with graded porosity have also been studied for their ability to better represent the actual in vivo situation where cells are exposed to layers of different tissues with varying properties. In addition, the ability of pore size and porosity of scaffolds to direct cellular responses and alter the mechanical properties of scaffolds will be reviewed, followed by a look at nature's own scaffold, the extracellular matrix. Overall, the limitations of current scaffold fabrication approaches for tissue engineering applications and some novel and promising alternatives will be highlighted.
Partial Orders for Efficient BMC of Concurrent Software (2013)

The vast number of interleavings that a concurrent program can have is typically identified as the root cause of the difficulty of automatic analysis of concurrent software. Weak memory is generally believed to make this problem even harder. We address both issues by modelling programs' executions with partial orders rather than the interleaving semantics (SC). We implemented a software analysis tool based on these ideas. It scales to programs of sufficient size to achieve first-time formal verification of non-trivial concurrent systems code over a wide range of models, including SC, Intel x86 and IBM Power.
HUMAN PLASMA PROTEINASE INHIBITORS (1983)

The Hippo pathway was initially discovered in Drosophila melanogaster as a key regulator of tissue growth. It is an evolutionarily conserved signaling cascade regulating numerous biological processes, including cell growth and fate decision, organ size ...Read More
Measuring Rates of Ubiquitin Chain Formation as a Functional Readout of Ligase Activity (2012)

Specificity within the pathways of ubiquitin conjugation are defined by protein-binding affinities among the components. Enzyme kinetics provides a facile high-resolution experimental approach for quantitating such protein-binding affinities and yields additional mechanistic insights into the transition state of the enzyme-catalyzed reaction. Most ubiquitin ligases form free polyubiquitin chains at a slow rate in the absence of their cognate target protein as a normal step in their overall catalytic cycle. Rates of polyubiquitin chain formation can, therefore, be used as a reporter function kinetically to characterize binding interactions within the ligation pathway. We describe experimental approaches for: (1) precisely quantitating functional E1 and E2 concentrations by their stoichiometric formation of (125)I-ubiquitin thiolester; (2) semiquantitative screens to define the cognate E2(s) for ubiquitin ligases based on their ability to support polyubiquitin chain formation; (3) initial rate studies to quantify K (m) and k (cat) as a measure of the ability of specific E2-ubiquitin thiolester substrates to support ligase-catalyzed polyubiquitin chain formation; and (4) an isopeptidase T-based technique for distinguishing between free and conjugated polyubiquitin chains formed in the functional assays. These kinetic methods provide mechanistic insights that are otherwise inaccessible by other experimental approaches and yield a precision in characterizing protein interactions that exceeds that of other techniques.
Günstigerer Verlauf des Herpes zoster bei immunsupprimierten Patienten unter Behandlung mit Fibroblasteninterferon (1984)

In a randomized study with patients suffering from herpes zoster spread of eruptions was inhibited and segmental pain was reduced rapidly by continuous infusion of daily 0.5 X 10(6) IE fibroblast interferon/kg body weight for 3-5 days. Postherpetic neuralgia was observed more rarely and interferon healing of eruptions was accelerated by interferon compared to the controls.
Ligands for natural killer cell receptors: redundancy or specificity (2001)

Several inhibitory and activating receptors involved in natural killer cell activation have been characterized. The increasing knowledge about their ligands, including classical MHC class I molecules, non-classical MHC class I molecules and MHC class I-related molecules, is shedding new light on the targets of innate immune recognition. While classical MHC class I molecules are constitutively expressed, some MHC class I-related (MIC) molecules, however, are stress-induced by ill-defined stimuli. Two families of ligands for the human activating NKG2D receptor have been identified. These are the MIC proteins encoded by two highly polymorphic genes within the MHC class I and the retinoic acid-inducible early gene-1-like (also designated UL16-binding) proteins encoded by genes outside the MHC. For the mouse NKG2D receptor, one family, containing at least five distinct ligands, has been described. A better understanding about how targets signal their distress, which renders them susceptible to natural killer (NK)-cell attack, will help to define the role of NK cells in antimicrobial and antitumor immunity and transplantation.
Neurogenic mechanisms in bronchial inflammatory diseases (2004)

Neurogenic inflammation encompasses the release of neuropeptides from airway nerves leading to inflammatory effects. This neurogenic inflammatory response of the airways can be initiated by exogenous irritants such as cigarette smoke or gases and is characterized by a bi-directional linkage between airway nerves and airway inflammation. The event of neurogenic inflammation may participate in the development and progression of chronic inflammatory airway diseases such as allergic asthma or chronic obstructive pulmonary disease (COPD). The molecular mechanisms underlying neurogenic inflammation are orchestrated by a large number of neuropeptides including tachykinins such as substance P and neurokinin A, or calcitonin gene-related peptide. Also, other biologically active peptides such as neuropeptide tyrosine, vasoactive intestinal polypeptide or endogenous opioids may modulate the inflammatory response and recently, novel tachykinins such as virokinin and hemokinins were identified. Whereas the different aspects of neurogenic inflammation have been studied in detail in laboratory animal models, only little is known about the role of airway neurogenic inflammation in human diseases. However, different functional properties of airway nerves may be used as targets for future therapeutic strategies and recent clinical data indicates that novel dual receptor antagonists may be relevant new drugs for bronchial asthma or COPD.
Temperature dependence of the thermoplastic formability in bulk metallic glasses (2011)

The temperature dependence of the maximum strain a bulk metallic glass (BMG) can undergo in its supercooled liquid state under specified conditions is determined. This formability is measured experimentally over a wide temperature range using Zr35Ti30Cu8.25Be26.75 as an example BMG. When considering five different BMG formers, we found that the formability continuously increases with temperature. This behavior is ubiquitous among BMGs whose crystallization behavior can be described by steady state nucleation and diffusion limited growth. Therefore, in order to maximize the formability during thermoplastic forming of BMGs, the highest processing temperature should be chosen at which crystallization can still be avoided.
Prolonged allergen challenge in mice leads to persistent airway remodelling (2004)

In conclusion, we have demonstrated that prolonged allergen challenge results in persistent airway wall remodelling.
Replicon clusters may form structurally stable complexes of chromatin and chromosomes (1994)

Nuclear DNA replication was monitored 'in situ' in pea nuclei with the bromodeoxyuridine antibody technique. The labelling appeared to be restricted to a number of finely distinct spots. The labelling was followed through three subsequent cell cycles in meristematic and differentiating pea root cells. The results show that the spots as seen just after the labelling persist distinctly over the mitotic chromosomes as well as in the nuclei of the following cell cycles up to 44 hours after the pulse. Moreover, they are also present in the nuclei of differentiating cells. The spots over the mitotic chromosomes in specific cases give rise to a dynamic banding. Nuclei of the second and third cycle show absence of labelling in specific zones, owing to the segregation of the labelled strands of chromosomal DNA. The maintenance of the spotted appearance of the replication clusters through all stages of the three subsequent cell cycles may be an indication in favour of the hypothesis that such clusters represent structurally stable replicon complexes held together by the nuclear matrix and the chromosome scaffold.
Effects of Types of Board Games on Spatial Abilities and Social Skills of Young Children (2003)

This study examined the differences in spatial abilities and social skills of young children using two different types of board games. Subjects consisted of 57 four year old children and 60 five year old children. Fifty eight were assigned to strategy-based board games, and 59 were assigned to chance-based board games. The same type of board game was given to each group. Each child played the board game 30 times. The results show that strategy-based board games have positive effects on spatial abilities, as well as on social skills in young children; there are age differences but no gender differences in both abilities. The findings of this study suggest that the spatial ability and social skills of young children can be increased using strategy-based games. This study proposes that early childhood educators need to consider the use of chance-based board games with a dice and spinner in preschool settings.
Infection and Autoimmunity as Etiologic Factors in Schizophrenia: A Review and Reappraisal (1993)

The focus of schizophrenia research has been turning from studies of structural and functional brain abnormalities to an increasing emphasis on possible etiologic factors. One etiologic hypothesis is that schizophrenia is the result of an infection (especially by a virus) or of an autoimmune reaction (perhaps following an infection) against central nervous system (CNS) tissue. Indirect evidence supporting this hypothesis includes possible geographic variance in the prevalence of schizophrenia, a season-of-birth effect, and observed associations between schizophrenia and prenatal exposure to viral epidemics. Several studies of cell-based and humoral immunity, as well as studies of cytokines, have indicated abnormalities in the immune function of schizophrenia patients, but many of these findings have not been replicated consistently. In addition, most observed alterations in immune function have been modest in degree and nonspecific. Attempts to identify a specific infectious agent or an antibody directed against CNS tissue have not produced a consistently replicable finding. In summary, no research evidence to date irrefutably indicates an infectious or autoimmune etiologic process in schizophrenia. It is probably unreasonable, however, to view schizophrenia as having a single cause. It is much more likely to be a heterogeneous disorder resulting from interactions between multiple factors, including the person's genetic endowment and various environmental influences. Infectious agents or CNS autoantibodies may well be among these environmental variables. A major current emphasis is on studying potential interactions between exposure to an infection or an autoimmune response and key early phases of brain development. A corresponding priority in the research agenda will be the development of animal models of CNS development that might elucidate the pathogenic mechanisms of such an interaction.
Complexes of Ras⋅GTP with Raf-1 and Mitogen-Activated Protein Kinase Kinase (1993)

The guanosine triphosphate (GTP)-binding protein Ras functions in regulating growth and differentiation; however, little is known about the protein interactions that bring about its biological activity. Wild-type Ras or mutant forms of Ras were covalently attached to an insoluble matrix and then used to examine the interaction of signaling proteins with Ras. Forms of Ras activated either by mutation (Gly12Val) or by binding of the GTP analog, guanylyl-imidodiphosphate (GMP-PNP) interacted specifically with Raf-1 whereas an effector domain mutant, Ile36Ala, failed to interact with Raf-1. Mitogen-activated protein kinase (MAP kinase) activity was only associated with activated forms of Ras. The specific interaction of activated Ras with active MAP kinase kinase (MAPKK) was confirmed by direct assays. Thus the forming of complexes containing MAPKK activity and Raf-1 protein are dependent upon the activity of Ras.
Investigation of near-infrared autofluorescence imaging for the detection of breast cancer (2005)

Detection of breast cancer in fresh tissue obtained from surgery is investigated using near-infrared autofluorescence imaging under laser excitation at 532 and 632.8 nm. The differences in intensity between the three main components of breast tissue (cancer, fibrous, and adipose) are estimated and compared to those obtained from cross-polarized light scattering images recorded under polarized illumination at 700 nm. The optical spectroscopic images for each tissue sample were subsequently compared with the histopathology slides. The experimental results indicate that the intensity of the near-infrared emission is considerably different in breast cancer compared to that of the adjacent nonneoplastic tissues (adipose and fibrous tissue). The experimental results suggest that 632.8-nm excitation offers key advantages compared to 532 nm excitation.
The roles of CD4 and CD8 in T cell activation. (1991)

CD4 and CD8 T cell surface molecules play a role in T cell recognition and activation by binding to their respective class II and class I major histocompatibility complex (MHC) ligands on an antigen presenting cell (APC). Though CD4 and CD8 are capable of binding to MHC molecules in the absence of the T cell receptor (TCR), increasing evidence suggests that they may primarily function by complexing with the TCR to form a 'co-receptor' for recognition of antigen-bound MHC. Using gene transfer studies we have demonstrated that CD4 and CD8 can augment antigen-induced IL-2 production through different mechanisms dependent on whether or not they can bind MHC independently of the TCR or complexed with the TCR. Under circumstances where CD4 and CD8 can bind to the same MHC ligand as the TCR, they potentiate antigen-induced IL-2 production maximally by a mechanism in large part dependent on their cytoplasmic tails. Enhancement of antigen-induced IL-2 production can also occur under circumstances where CD4 and CD8 bind on MHC ligand distinct from that recognized by the TCR. In this instance, the magnitude of this enhancement is not as great and appears (at least for CD8) to be independent of the cytoplasmic tail and the associated p56lck. The dependence of co-receptor function on the cytoplasmic tail of CD4 or CD8 may reflect the activity of the associated intracellular tyrosine kinase p56lck.(ABSTRACT TRUNCATED AT 250 WORDS)
Abdominal Aortic Aneurysm Morphology in Candidates for Endovascular Repair Evaluated With Spiral Computed Tomography and Digital Subtraction Angiography (1999)

AAA anatomy varies widely and independently of the aneurysm size. Therefore, the maximum size of the aneurysm is a poor predictor of whether or not an aneurysm is suitable for endovascular repair. The discrepancy between angiographic and axial CT measurements suggests that neither alone is sufficient as a preoperative imaging technique when evaluating a patient for an endovascular graft procedure.
Novel Synthesis and Structural Analysis of Ferrihydrite (2012)

Naturally occurring ferrihydrite is both impure and difficult to isolate, so the numerous applications and interesting properties of ferrihydrite have spurred the development of various synthetic techniques. Nearly all techniques are based on the hydrolysis of an iron salt and require careful control of temperature, pH, and concentration. In this Article, we report a new synthetic method which does not require such control and is perhaps the fastest and simplest route to synthesizing ferrhydrite. XRD, TEM, BET, and chemical purity characterizations show that the chemically pure, 2-line ferrihydrite product consists of crystallites 2-6 nm in diameter which aggregate to form mesoporous, high surface area agglomerates that are attractive candidates for the many adsorption applications of ferrihydrite. X-ray PDF data were also collected for the ferrihydrite product and refined against the hexagonal structural model recently proposed by Michel et al. These analyses suggest that ferrihydrite has a consistent, repeatable structure independent of variation in the synthetic method, water content of the sample, or particle size of the crystallites, and this structure can be adequately described by the proposed hexagonal model.