gebhart/patrepeval huggingface dataset:
A New Heparin‐Bonded Dense Membrane Lung Combined with Minimal Systemic Heparinization Prolonged Extracorporeal Lung Assist in Goats (1998)

Heparin was covalently bonded to a new hollow-fiber dense membrane artificial lung and extracorporeal circuit using a silane coupling agent and polyethyleneimine. This study investigated whether prolonged, venoarterial bypass extracorporeal lung assist (V-A bypass ECLA) could be sustained in a goat by the combination of the new membrane lung and minimal systemic heparinization. We maintained ECLA with the hollow-fiber lungs (surface area, 0.8 m2) and circuits by titrating the activated clotting time (ACT) to below 150 s with minimal systemic heparinization in 5 goats. The outcome was assessed from the function of the artificial lung via macro and microscopic examinations after the experiments and the incidence of systemic complications. The 5 goats were maintained on ECLA for 6 to 27 days. The bypass flow rate, blood gases at the return and drainage sites, platelet counts, and platelet aggregation activity were well maintained. Although the hemoglobin concentration, hematocrit, and plasma protein at the start of the ECLA were significantly lower than the pre-ECLA values due to hemodilution, the values remained stable during ECLA. A cerebral infarction occurred in 1 goat. However, in the other 4 goats, no complications such as bleeding, thrombosis, or plasma leakage from the artificial lung were observed. Although several thrombi were observed in the stagnant area of the artificial lung, these local thrombi did not cause the function of the artificial lung to deteriorate. We found that this new type of highly biocompatible, dense membrane artificial lung, when combined with minimal systemic heparinization, prolonged ECLA without the deterioration of the artificial lung function and was suitable for prolonged ECLA.
Body Metabolism Provides a Foundation for Noninvasive Blood Glucose Monitoring (2004)

The metabolic oxidation of glucose in the human body, also known as cellular respiration, provides most of the energy necessary for cellular activities. Several authors (1–4) have suggested that the relationship between blood glucose concentration and physiological parameters relates to metabolism. Until now, this phenomenon has not been utilized to calculate blood glucose concentration (5–7). Here we describe the noninvasive measurements of glucose concentration in the blood by taking into account the body heat generated by glucose oxidation and local oxygen supply. We arrived at this concept from observing that the circadian rhythm of the human body conforms to the subtle balance seen among metabolic heat, local oxygen supply, and glucose concentration. We have developed a system that integrates both thermal and spectroscopic sensors, which simultaneously measures these related parameters and subsequently physicochemically derives the concentration of blood glucose. Our discovery shows that blood glucose concentration can be accurately and reliably measured by this novel, noninvasive technique.

Our concept is …
Biosynthesis of the carbohydrate portion of immunoglobulin radiochemical and chemical analysis of the carbohydrate moieties of two myeloma proteins purified from different subcellular fractions of plasma cells (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBiosynthesis of the carbohydrate portion of immunoglobulin radiochemical and chemical analysis of the carbohydrate moieties of two myeloma proteins purified from different subcellular fractions of plasma cellsFritz MelchersCite this: Biochemistry 1971, 10, 4, 653–659Publication Date (Print):February 16, 1971Publication History Published online1 May 2002Published inissue 16 February 1971https://doi.org/10.1021/bi00780a017Request reuse permissionsArticle Views34Altmetric-Citations55LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (759 KB) Get e-Alertsclose Get e-Alerts
Novel single molecule precursor routes for the direct synthesis of highly monodispersed quantum dots of cadmium or zinc sulfide or selenide (1998)

Good quality highly mono-dispersed nanoparticles of II–VI binary chalcogenides ME (M = Zn or Cd, E = S or Se) have been prepared in a ‘one-pot’ synthesis by the thermolysis in tri-n-octylphosphine oxide (TOPO, e.g. at 200 °C for 45 min, ca. 50 Å ± 2.5 Å diameter) of the corresponding bis[methyl(n-hexyl)di-thio or -seleno]carbamato {M[E2CNMe(C6H13)]2, E = S or Se} complexes; the CdS, CdSe and ZnSe particles have been characterized by electronic spectroscopy, photoluminiscence, X-ray diffraction and electron microscopy (SAED, SEM and TEM).
Survival and prognosis among 1545 patients with contemporary polycythemia vera: an international study (2013)

Under the auspices of an International Working Group, seven centers submitted diagnostic and follow-up information on 1545 patients with World Health Organization-defined polycythemia vera (PV). At diagnosis, median age was 61 years (51% females); thrombocytosis and venous thrombosis were more frequent in women and arterial thrombosis and abnormal karyotype in men. Considering patients from the center with the most mature follow-up information (n=337 with 44% of patients followed to death), median survival (14.1 years) was significantly worse than that of the age- and sex-matched US population (P<0.001). In multivariable analysis, survival for the entire study cohort (n=1545) was adversely affected by older age, leukocytosis, venous thrombosis and abnormal karyotype; a prognostic model that included the first three parameters delineated risk groups with median survivals of 10.9-27.8 years (hazard ratio (HR), 10.7; 95% confidence interval (CI): 7.7-15.0). Pruritus was identified as a favorable risk factor for survival. Cumulative hazard of leukemic transformation, with death as a competing risk, was 2.3% at 10 years and 5.5% at 15 years; risk factors included older age, abnormal karyotype and leukocytes ≥15 × 10(9)/l. Leukemic transformation was associated with treatment exposure to pipobroman or P32/chlorambucil. We found no association between leukemic transformation and hydroxyurea or busulfan use.
Loss-of-function polymorphisms in the filaggrin gene are associated with an increased susceptibility to chronic irritant contact dermatitis: a case-control study (2008)

Our study shows that FLG null alleles are associated with increased susceptibility to CICD; whether or not the FLG null allele is an independent risk factor needs further study.
Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis (2008)

Seeds represent the main source of nutrients for animals and humans, and knowledge of their biology provides tools for improving agricultural practices and managing genetic resources. There is also tremendous interest in using seeds as a sustainable alternative to fossil reserves for green chemistry. Seeds accumulate large amounts of storage compounds such as carbohydrates, proteins and oils. It would be useful for agro-industrial purposes to produce seeds that accumulate these storage compounds more specifically and at higher levels. The main metabolic pathways necessary for oil, starch or protein accumulation are well characterized. However, the overall regulation of partitioning between the various pathways remains unclear. Such knowledge could provide new molecular tools for improving the qualities of crop seeds (Focks and Benning, 1998, Plant Physiol. 118, 91). Studies to improve understanding of the genetic controls of seed development and metabolism therefore remain a key area of research. In the model plant Arabidopsis, genetic analyses have demonstrated that LEAFY COTYLEDON genes, namely LEC1, LEC2 and FUSCA3 (FUS3), are key transcriptional regulators of seed maturation, together with ABSCISIC ACID INSENSITIVE 3 (ABI3). Interestingly, LEC2, FUS3 and ABI3 are related proteins that all contain a 'B3' DNA-binding domain. In recent years, genetic and molecular studies have shed new light on the intricate regulatory network involving these regulators and their interactions with other factors such as LEC1, PICKLE, ABI5 or WRI1, as well as with sugar and hormonal signaling. Here, we summarize the most recent advances in our understanding of this complex regulatory network and its role in the control of seed maturation.
Water-in-Carbon Dioxide Microemulsions with a Fluorocarbon-Hydrocarbon Hybrid Surfactant (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTWater-in-Carbon Dioxide Microemulsions with a Fluorocarbon-Hydrocarbon Hybrid SurfactantKristi Harrison, Jacqueline Goveas, Keith P. Johnston, and Edgar A. O'Rear IIICite this: Langmuir 1994, 10, 10, 3536–3541Publication Date (Print):October 1, 1994Publication History Published online1 May 2002Published inissue 1 October 1994https://pubs.acs.org/doi/10.1021/la00022a028https://doi.org/10.1021/la00022a028research-articleACS PublicationsRequest reuse permissionsArticle Views700Altmetric-Citations252LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Characterization of plasma lipoproteins separated and purified by agarose-column chromatography (1974)

1. A simple method for isolation of individual human plasma lipoprotein classes is presented. In this technique, lipoproteins are removed from plasma at d1.225 by ultracentrifugation, after which they are separated and purified by agarose-column chromatography. 2. Three major classes are obtained after agarose-column chromatography. Separation between classes is excellent; more than 95% of the lipoproteins eluted from the column are recovered in the form of a purified lipoprotein class. 3. Each lipoprotein class was characterized immunologically, chemically, electrophoretically and by electron microscopy. A comparison of the properties of the column-isolated lipoproteins was made with very-low-density lipoproteins, low-density lipoproteins, and high-density lipoproteins separated by sequential ultracentrifugation at densities of 1.006, 1.063 and 1.21 respectively. 4. By each criterion, peak-I lipoproteins from the agarose column are the same as very-low-density lipoproteins, peak-II lipoproteins are the same as low-density lipoproteins, and peak-III lipoproteins are the same as high-density lipoproteins. Thus the lipoprotein classes isolated by both methods are similar if not identical. 5. The agarose-column separation technique offers the advantage of a two- to three-fold saving in time. In addition, the column-elution pattern serves as a recording of the size distribution of lipoproteins in plasma. 6. The most complete characterization is reported for human plasma lipoproteins. The results with rhesus-monkey and rabbit lipoproteins were identical.
Wordspotting for voice editing and audio indexing (1992)

No abstract available.
Relaxation on a mesh: a formalism for generalized localization (2002)

This paper considers two problems which at first sight appear to be quite distinct: localizing a robot in an unknown environment and calibrating an embedded sensor network. We show that both of these can be formulated as special cases of a generalized localization problem. In the standard localization problem, the aim is to determine the pose of some object (usually a mobile robot) relative to a global coordinate system. In our generalized version, the aim is to determine the pose of all elements in a network (both fixed and mobile) relative to an arbitrary global coordinate system. We have developed a physically inspired 'mesh-based' formalism for solving such problems. This paper outlines the formalism, and describes its application to the concrete tasks of multirobot mapping and calibration of a distributed sensor network. The paper presents experimental results for both tasks obtained using a set of Pioneer mobile robots equipped with scanning laser range-finders.
Anticoagulant peptides. Nature of the interaction of the C-terminal region of hirudin with a noncatalytic binding site on thrombin (1987)

A series of 20 C-terminal fragment analogues of the anticoagulant peptide hirudin were synthesized by solid-phase techniques in order to investigate the nature of the thrombin-hirudin interaction. Inhibition of plasma fibrin clot formation by thrombin in vitro was used as a measure of anticoagulant activity. In the minimum region necessary for detectable anticoagulant activity, hirudin56-64, positions Phe56, Glu57, Ile59, Pro60, and Leu64 are sensitive to modification. These residues are apparently important for direct interaction with thrombin or for maintaining a favorable conformation for the interaction. On the basis of conformational analysis of this region by computational methods, a "kinked" amphipathic alpha-helical structure, which orients all of the residues most critical for activity on one face of the helix, is proposed.
Structure of glycerol-3-phosphate dehydrogenase (GPD1) fromSaccharomyces cerevisiaeat 2.45 Å resolution (2012)

The interconversion of glycerol 3-phosphate and dihydroxyacetone phosphate by glycerol-3-phosphate dehydrogenases provides a link between carbohydrate and lipid metabolism and provides Saccharomyces cerevisiae with protection against osmotic and anoxic stress. The first structure of a glycerol-3-phosphate dehydrogenase from S. cerevisiae, GPD1, is reported at 2.45 Å resolution. The asymmetric unit contains two monomers, each of which is organized with N- and C-terminal domains. The N-terminal domain contains a classic Rossmann fold with the (β-α-β-α-β)2 motif typical of many NAD+-dependent enzymes, while the C-terminal domain is mainly α-helical. Structural and phylogenetic comparisons reveal four main structure types among the five families of glycerol-3-phosphate and glycerol-1-phosphate dehydrogenases and reveal that the Clostridium acetobutylican protein with PDB code 3ce9 is a glycerol-1-phosphate dehydrogenase.
Evidence for the expression of a second CD6 ligand by synovial fibroblasts (2000)

Taken together with previous data regarding CD6 and CD166 function, the present findings strongly suggest the existence of a second CD6 ligand distinct from CD166, which can be expressed by synovial fibroblasts as well as other cells.
MassBank: a public repository for sharing mass spectral data for life sciences (2010)

MassBank is the first public repository of mass spectra of small chemical compounds for life sciences (<3000 Da). The database contains 605 electron-ionization mass spectrometry (EI-MS), 137 fast atom bombardment MS and 9276 electrospray ionization (ESI)-MS(n) data of 2337 authentic compounds of metabolites, 11 545 EI-MS and 834 other-MS data of 10,286 volatile natural and synthetic compounds, and 3045 ESI-MS(2) data of 679 synthetic drugs contributed by 16 research groups (January 2010). ESI-MS(2) data were analyzed under nonstandardized, independent experimental conditions. MassBank is a distributed database. Each research group provides data from its own MassBank data servers distributed on the Internet. MassBank users can access either all of the MassBank data or a subset of the data by specifying one or more experimental conditions. In a spectral search to retrieve mass spectra similar to a query mass spectrum, the similarity score is calculated by a weighted cosine correlation in which weighting exponents on peak intensity and the mass-to-charge ratio are optimized to the ESI-MS(2) data. MassBank also provides a merged spectrum for each compound prepared by merging the analyzed ESI-MS(2) data on an identical compound under different collision-induced dissociation conditions. Data merging has significantly improved the precision of the identification of a chemical compound by 21-23% at a similarity score of 0.6. Thus, MassBank is useful for the identification of chemical compounds and the publication of experimental data.
Water quality and occurrences of protozoa and metazoa in two constructed wetlands treating different wastewaters in Thailand (1997)

Two constructed wetlands were evaluated regarding their performance in treating different wastewaters under the tropical conditions of Thailand. The first one was for treating the domestic sewage from a governmental institute, whereas the latter was used to improve the quality of the effluent from a central aerated lagoon, for which the influent had been already pretreated by about 100 factories in an industrial estate. The hydraulic loading rate and the hydraulic retention time were 0.015 m3/m2.d and 28 days and 0.3 m3/m2.d and 3 days for the two wetland systems, respectively. The physical and chemical water quality and the occurrences of protozoa and metazoa were monitored for three seasons, each of which had samples taken twice a week for two months. The temperature was quite constant throughout the whole year, ie., 26 to 31°C and 25 to 34°C for the two wetlands, respectively. The water quality of the wetland effluents was rather good; the BOD and TKN were only 4.5 and 3.3 mg/l and 2.7 and 6.8 mg/l for the two cases. A relationship between the filtered BOD and the zoological characteristics was established. As a result, a simple glance at the occurrence of certain metazoa and protozoa can rapidly but approximately predict the water quality of the system.
Next-generation gene targeting in the mouse for functional genomics (2009)

In order to elucidate ultimate biological function of the genome, the model animal system carrying mutations is indispensable. Recently, large-scale mutagenesis projects have been launched in various species. Especially, the mouse is considered to be an ideal model to human because it is a mammalian species accompanied with well-established genetic as well as embryonic technologies. In 1990', large-scale mouse mutagenesis projects firstly initiated with a potent chemical mutagen, N-ethyl-N-nitrosourea (ENU) by the phenotype-driven approach or forward genetics. The knockout mouse mutagenesis projects with trapping/conditional mutagenesis have then followed as Phase II since 2006 by the gene-driven approach or reverse genetics. Recently, the next-generation gene targeting system has also become available to the research community, which allows us to establish and analyze mutant mice carrying an allelic series of base substitutions in target genes as another reverse genetics. Overall trends in the large-scale mouse mutagenesis will be reviewed in this article particularly focusing on the new advancement of the next-generation gene targeting system. The drastic expansion of the mutant mouse resources altogether will enhance the systematic understanding of the life. The construction of the mutant mouse resources developed by the forward and reverse genetic mutagenesis is just the beginning of the annotation of mammalian genome. They provide basic infrastructure to understand the molecular mechanism of the gene and genome and will contribute to not only basic researches but also applied sciences such as human disease modelling, genomic medicine and personalized medicine.
The Design of Impedance-Matching Networks for Radio-Frequency and Microwave Amplifiers (1985)

The design of impedance-matching networks for radio-frequency and microwave amplifiers , The design of impedance-matching networks for radio-frequency and microwave amplifiers , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
TMPRSS2–ERG gene fusion prevalence and class are significantly different in prostate cancer of caucasian, african‐american and japanese patients (2010)

The prevalence and class of TMPRSS2-ERG are significantly different in PCa of Caucasian, African-American, and Japanese patients. Future studies of the molecular pathways implicated in TMPRSS2-ERG gene fusion may shed light on the disparity in prevalence and mortality of PCa among different ethnic groups and help design better prevention and treatment strategies.
水稻 ATP-Binding Cassette (ABC) 轉運蛋白OsABCG36 的功能性分析 (2011)

ABC 轉運蛋白 (ATP-Binding Cassette transporter) 為一廣泛存在於各物種的龐大膜轉運蛋白家族，在多種基質的運輸上扮演重要角色，包括胺基酸、多肽鏈、脂質及金屬離子等。水稻具有超過130個 ABC 轉運蛋白，但對其功能了解非常少。我們過去的研究發現，屬於水稻PDR類的ABC轉運蛋白基因OsABCG36，其表現受到多種重金屬高度誘導。為瞭解其在水稻中扮演的角色，本研究進一步針對OsABCG36進行功能性分析。酵母菌功能性分析結果顯示，大量表現 OsABCG36 會專一性的促進鈷顯著累積而對酵母菌造成毒害。水稻功能性分析顯示，OsABCG36-RNAi基因靜默水稻對於鈷具有較高的敏感性；相反地，OsABCG36-OE 大量表現水稻具有較高的鈷耐受性。鈷累積量分析發現不論OsABCG36-RNAi及OsABCG36-OE都與野生型無明顯差異。次細胞定位結果顯示，OsABCG36-GFP融合蛋白可能位於葉綠體與細胞膜上。基因表現分析結果顯示，OsABCG36 基因表現會隨著鈷處理濃度和時間而受到大幅誘導，其中梗稻TNG67和秈稻TN1根部以2.5 mM氯化鈷處理，可分別誘導856倍及2536倍，在地上部則分別誘導22倍及9.8倍。啟動子特性分析顯示，POsABCG36(1K)/GUS、POsABCG36(1.5K)/GUS以及POsABCG36(2K)/GUS轉殖水稻在葉、葉環、葉鞘及節皆有GUS表現，並受鈷誘導。分析隱子 (intron) 對OsABCG36啟動子的活性影響發現，第一個隱子可增加重金屬誘導性，第二個隱子可增強啟動子在葉部活性，第三個隱子則抑制啟動子活性。以鈷處理水稻幼苗發現，高濃度的鈷會造成水稻的毒害，並抑制水稻幼苗根部的發育。以上結果顯示，OsABCG36是一個鈷轉運蛋白，在酵母菌中可能為一個鈷吸收轉運蛋白，在植物中，OsABCG36可能位於葉綠體與細胞膜上，並影響細胞內的鈷分布
Sites of interaction of the CCA end of peptidyl-tRNA with 23S rRNA. (1991)

Oligonucleotide fragments derived from the 3' CCA terminus of acylated tRNA, such as CACCA-(AcPhe), UACCA-(AcLeu), and CAACCA-(fMet), bind specifically to ribosomes in the presence of sparsomycin and methanol [Monro, R. E., Celma, M. L. & Vazquez, D. (1969) Nature (London) 222, 356-358]. All three oligonucleotides protect a characteristic set of bases in 23S rRNA from chemical probes: G2252, G2253, A2439, A2451, U2506, and U2585. A2602 shows enhanced reactivity. These account for most of the same bases that are protected when peptidyl-tRNA analogues such as AcPhe-tRNA are bound to the ribosomal P site, and correspond precisely to those bases whose protection is abolished by removal of the 3'-CA end of tRNA. We conclude that most of the observed interactions between tRNA and 23S rRNA in the 50S ribosomal P site involve the conserved CCA terminus of tRNA. Sparsomycin may inhibit protein synthesis by stabilizing interaction between the peptidyl-CCA and the 23S P site, preventing formation of the intermediate A/P hybrid state.