gebhart/patrepeval huggingface dataset:
Toward Repositioning Niclosamide for Antivirulence Therapy of Pseudomonas aeruginosa Lung Infections: Development of Inhalable Formulations through Nanosuspension Technology (2015)

Inhaled antivirulence drugs are currently considered a promising therapeutic option to treat Pseudomonas aeruginosa lung infections in cystic fibrosis (CF). We have recently shown that the anthelmintic drug niclosamide (NCL) has strong quorum sensing (QS) inhibiting activity against P. aeruginosa and could be repurposed as an antivirulence drug. In this work, we developed dry powders containing NCL nanoparticles that can be reconstituted in saline solution to produce inhalable nanosuspensions. NCL nanoparticles were produced by high-pressure homogenization (HPH) using polysorbate 20 or polysorbate 80 as stabilizers. After 20 cycles of HPH, all formulations showed similar properties in the form of needle-shape nanocrystals with a hydrodynamic diameter of approximately 450 nm and a zeta potential of -20 mV. Nanosuspensions stabilized with polysorbate 80 at 10% w/w to NCL (T80_10) showed an optimal solubility profile in simulated interstitial lung fluid. T80_10 was successfully dried into mannitol-based dry powder by spray drying. Dry powder (T80_10 DP) was reconstituted in saline solution and showed optimal in vitro aerosol performance. Both T80_10 and T80_10 DP were able to inhibit P. aeruginosa QS at NCL concentrations of 2.5-10 μM. NCL, and these formulations did not significantly affect the viability of CF bronchial epithelial cells in vitro at microbiologically active concentrations (i.e., ≤10 μM). In vivo acute toxicity studies in rats confirmed no observable toxicity of the NCL T80_10 DP formulation upon intratracheal administration at a concentration 100-fold higher than the anti-QS activity concentration. These preliminary results suggest that NCL repurposed in the form of inhalable nanosuspensions has great potential for the local treatment of P. aeruginosa lung infections as in the case of CF patients.
A rapid noninvasive blood pressure measurement method for discrete value and full waveform determination (1996)

A new noninvasive measurement method providing rapid measurement of systemic arterial blood pressure (BP) and its validation is described. The method combines precisely timed electrocardiographic-gated rapid release of occluding counter-pressure (600 mmHg/s) with photoplethysmographic detection of radial artery filling to measure arterial opening pressure. A complete BP waveform is reconstructed from multiple repetitions of the measurement cycle at successively increasing time intervals relative to the electrocardiographic signal. Systolic and diastolic values can be measured within two to four cardiac cycles at the peak and trough of the BP wave. The new method was compared with sphygmomanometry in 26 randomly selected subjects over a sphygmomanometric pressure range of 53-110 (diastolic) and 100-190 mmHg (systolic). The mean pressure differences between the sphygmomanometric and new methods were -1.3 +/- 15.2 (SD) (systolic) and 0.7 +/- 9.9 mmHg (diastolic), and corresponding BP values measured by these methods were highly correlated [P < 0.001; R2 = 0.87 (systolic); R2 = 0.80 (diastolic)]. The new method was compared with sphygmomanometry and intra-arterial BP in six patients. These tests confirmed the method's validity compared with established methods. The new method was ostensibly immune to mechanical perturbations when tested during cycle ergometry at 60 W. The new method may facilitate the study of circulatory phenomena previously inaccessible by available noninvasive methods and minimizes patient discomfort and circulatory arrest at the measurement site.
Robust execution of temporally flexible plans for bipedal walking devices (2006)

Robotic wheeled rovers have been successfully controlled by activity execution systems that use plans with temporal flexibility to adapt to disturbances. These systems abstract away the detailed dynamic constraints of the controlled device. To control dynamic, devices, such as agile bipeds, we extend this execution paradigm to incorporate detailed dynamic constraints.

Building upon prior work on dispatchable plan execution, we introduce a novel approach to flexible plan execution that achieves robustness by exploiting spatial as well as temporal plan flexibility. To accomplish this, we first transform the high-dimensional system into a set of low dimensional, weakly-coupled systems. Second, to coordinate these systems, we compile a plan into a flow tube description, representing all legal trajectories and their temporal coordination. Finally, the problem of runtime plan dispatching is reduced to maintaining state trajectories in their associated flow tubes. The approach is validated using a high fidelity biped simulation.
A non-orthogonal coupled mode theory for super-modes inside multi-core fibers (2014)

In this paper, a non-orthogonal coupled mode theory is proposed to analyze the super-modes of multi-core fibers (MCFs). The theory is valid in the strong coupling regime and can provide accurate analytical formulas for the super-modes inside MCFs. MCFs with circularly distributed cores are analyzed as an example. Analytical formulas are derived both for the refractive indexes and the eigen vectors of the super-modes. It is rigorously revealed that the eigen vectors for the super-modes of such MCFs are the row vectors of the inverse discrete Fourier transform (IDFT) matrix. Therefore, by pre-coding the signal channels via IDFT, one is able to generate the super-modes for the MCFs with circularly distributed cores.
Biotin-functionalized self-assembled monolayers on gold: surface plasmon optical studies of specific recognition reactions (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBiotin-functionalized self-assembled monolayers on gold: surface plasmon optical studies of specific recognition reactionsL. Haeussling, H. Ringsdorf, F. J. Schmitt, and W. KnollCite this: Langmuir 1991, 7, 9, 1837–1840Publication Date (Print):September 1, 1991Publication History Published online1 May 2002Published inissue 1 September 1991https://pubs.acs.org/doi/10.1021/la00057a001https://doi.org/10.1021/la00057a001research-articleACS PublicationsRequest reuse permissionsArticle Views1873Altmetric-Citations227LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The impact of improved phosphorus control: use of sevelamer hydrochloride in patients with chronic renal failure (2002)

Phosphorus control is a primary goal in the care of patients with end-stage renal disease (ESRD). Sevelamer hydrochloride, a novel calcium-free, aluminum-free phosphate binder, allows physicians to control serum phosphorus in patients with ESRD without increasing serum calcium levels or contributing an excess calcium load. Clinical studies have shown that sevelamer provides sustained reduction in markers of soft-tissue and cardiac calcification, specifically serum phosphorus, calciumxphosphorus product, parathyroid hormone and also improves blood lipid profiles. Thus, sevelamer hydrochloride offers the promise of impacting cardiac calcification and thereby reducing patient morbidity and mortality. Long-term studies are underway to evaluate these potential benefits. This paper reviews sevelamer studies to date and addresses ongoing strategies for improving clinical management of phosphorus in ESRD.
Presentation of the Self Antigen Myelin Basic Protein by Dendritic Cells Leads to Experimental Autoimmune Encephalomyelitis (1999)

Bone marrow (BM)-derived dendritic cells (DC) are potent stimulators of naive CD4+ T cell activation. Because DC are efficient at Ag processing and could potentially present self Ags, we investigated the role of DC in the presentation of an encephalitogenic peptide from myelin basic protein (Ac1-11) in the induction of experimental autoimmune encephalomyelitis (EAE). To determine if DC could prime for EAE, we transferred DC pulsed with Ac1-11 or with medium alone into irradiated mice in combination with CD4+ T cells isolated from a mouse transgenic for a TCR specific for Ac1-11 + I-Au. Mice transferred with Ac1-11-pulsed DC developed EAE 7-10 days later, whereas mice receiving medium-pulsed DC did not. By day 15, all mice given peptide-loaded DC had signs of tail and hind limb paralysis, and by day 20 infiltration of Ac1-11-specific CD4+ T cells was detected in the brain parenchyma. We also demonstrated interactions between Ac1-11-pulsed DC and Ac1-11-specific T cells in the lymph nodes 24 h following adoptive transfer of both cell populations. These data show that DC can efficiently present the self Ag myelin basic protein Ac1-11 to Ag-specific T cells in the periphery of mice to induce EAE.
Ultra-high speed AlGaAs/GaAs heterojunction bipolar transistors (2003)

The characteristic device performance of AlGaAs/GaAs ballistic collection transistors (BCTs) and applications of this device to integrated circuits are considered. The BCT structure reduces the collector transit times, which leads to an increase in cutoff frequency. Although near-ballistic collection only occurs in a relatively narrow collector voltage range, the cutoff frequency can be maximized at a desired collector voltage. This feature is useful when applying BCTs to logic gates. A propagation delay time of 1.9 ps/gate has been obtained in an ECL (emitter coupled logic) ring oscillator configured of BCTs. The maximum toggle frequency of a one-by-two frequency divider consisting of eight NOR-gates is as high as 20.2 GHz. These results demonstrate the high-speed potential of BCTs.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A fast distributed network restoration algorithm (2002)

The authors present a fast distributed network restoration algorithm for restoring disrupted traffic in a digital cross-connect system (DCS) based fiber network due to fiber span cut. The proposed algorithm is based on a two-prong approach where both disrupted ends simultaneously send out restore messages as opposed to the conventional approaches where one end is selected as the sender and only the sender sends out restore messages. It is shown that the proposed algorithm outperforms those distributed network restoration algorithms based on the sender-chooser approach, and are capable of rerouting the disrupted traffic in sub-seconds. The simulation results of the performance of the new algorithm and other known distributed network restoration algorithms are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A conceptual study of OFDM-based multiple access schemes: Part 2 - Channel estimation in the uplink (1996)

This paper deals with the design of channel estimators in the uplink of an OFDM based multi-user system. First, we propose a user allocation scheme that is based on the aim to use the correlation of the radio channel for channel estimation purposes. Further, we present the linear MMSE channel estimator based on a few pilot tones. We present an error analysis that proves valuable in the evaluation of linear channel estimators under mismatch. Finally, we present some results on the design of linear channel estimators and pilot patterns, showing by means of examplesthe importance of careful pilot positioning.
Effects of Gamma Irradiation on the Transduction of Dividing and Nondividing Cells in Brain and Muscle of Rats by Adeno-Associated Virus Vectors (1996)

Vectors based on adeno-associated virus (AAV) are under investigation for use in gene therapy applications. Critical aspects of AAV vector biology remain undefined, in particular the intracellular events and activities mediating transduction and determining host cell permissiveness for transduction. Using cultured primary human fibroblasts, we previously showed that AAV vectors preferentially, but not exclusively, transduce cells in the S phase of the cell cycle, and that transduction can be markedly enhanced by pretreatment of target cells with physical and chemical agents that perturb DNA metabolism. In this study, we tested whether similar improvements in AAV vector performance might be achievable in vivo. The adult rat brain and overlying scalp muscle were selected for vector inoculation because of the presence of well-defined populations of dividing, quiescent, and post-mitotic cells, and gamma irradiation was chosen as a reproducible means of inducing DNA repair in these cells. We find that gamma irradiation markedly enhances the transduction of dividing cell populations in the pia-arachnoid and choroid epithelium within the central nervous system, and of mature nondividing muscle cells in the scalp, whereas gamma irradiation did not increase the basal transduction level of post-mitotic neurons in the hippocampus. These data confirm that replicative cellular DNA synthesis is not required for transduction by AAV vectors and show that the mitotic state of target cells is not necessarily predictive of responsiveness to transduction-enhancing treatments. Most importantly, these data demonstrate that target cells can be manipulated in vivo to render them more permissive for AAV vector transduction.
Cortisol directly determined in serum by fluoroimmunoassay with magnetizable solid phase. (1980)

We developed a direct fluoroimmunoassay for cortisol in serum. This method involves cortisol labeled at the 3 position with fluorescein, and antibodies to cortisol coupled to magnetizable cellulose/iron oxide particles. Sodium salicylate is used as a blocking agent to prevent interference from endogenous binding proteins in serum. Serum sample and labeled cortisol are incubated with the antibody-coupled solid phase for 30 min. The solid phase is then separated and washed to remove free labeled cortisol and endogenous fluorophores of the sample. Finally we elute the antibody-bound fraction of the labeled cortisol into an alkaline methanolic medium and measure its fluorescence. The separation, wash, and elution steps are facilitated by magnetic sedimentation. The assay is sufficiently sensitive, specific, and reliable for routine use and correlates acceptably (r = 0.92) with an established radioimmunoassay. Precision (CV) ranged from 4 to 10% in experiments on three pooled sera; analytical recovery for sera supplemented with as much as 360 microgram of cortisol per liter was 91 to 109%.
Morphology and Properties of Polyester/Exfoliated Graphite Nanocomposites (2008)

Nanocomposites reinforced with graphite platelets were compared to those with functionalized graphite sheets (FGS) prepared by partial pyrolysis of graphite oxide. Melt dispersion in poly(ethylene-2,6-naphthalate) (PEN) was quantified using a range of characterization techniques: electron microscopy, X-ray scattering, melt rheology, electrical conductivity, gas barrier, and mechanical properties. Conductivity percolation was obtained with as little as 0.3 vol % FGS, whereas 3 vol % was required for graphite. The threshold concentrations of FGS and graphite for rigidity percolation determined with melt rheology were in good agreement with conductivity percolation. Hydrogen permeability of PEN with 4 wt % FGS was decreased by 60% while the same amount of graphite reduced permeability only 25%. Structural differences between graphite and FGS were characterized with atomic force microscopy (AFM), Raman spectroscopy, and X-ray photoelectron spectroscopy (XPS). The highly exfoliated morphology of FGS was maintained in the composites as revealed by electron microscopy and X-ray scattering while graphite layers remained stacked together even after melt processing. Even though the tensile stiffness and dimensional stability of PEN were improved, the extent of reinforcement with FGS for these two properties was not as significant. This was attributed to the wrinkled structure of FGS and atomistic defects.
CD27, a Member of the Tumor Necrosis Factor Receptor Superfamily, Activates NF-κB and Stress-activated Protein Kinase/c-Jun N-terminal Kinase via TRAF2, TRAF5, and NF-κB-inducing Kinase (1998)

CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily and is expressed on T, B, and NK cells. The signal via CD27 plays pivotal roles in T-T and T-B cell interactions. Here we demonstrate that overexpression of CD27 activates NF-kappaB and stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK). Deletion analysis of the cytoplasmic domain of CD27 revealed that the C-terminal PIQEDYR motif was indispensable for both NF-kappaB and SAPK/JNK activation and was also required for the interaction with TNF receptor-associated factor (TRAF) 2 and TRAF5, both of which have been implicated in NF-kappaB activation by members of the TNF-R superfamily. Co-transfection of a dominant negative TRAF2 or TRAF5 blocked NF-kappaB and SAPK/JNK activation induced by CD27. Recently, a TRAF2-interacting kinase has been identified, termed NF-kappaB-inducing kinase (NIK). A kinase-inactive mutant NIK blocked CD27-, TRAF2-, and TRAF5-mediated NF-kappaB and SAPK/JNK activation. These results indicate that TRAF2 and TRAF5 are involved in NF-kappaB and SAPK/JNK activation by CD27, and NIK is a common downstream kinase of TRAF2 and TRAF5 for NF-kappaB and SAPK/JNK activation.
Liver repopulation for the treatment of metabolic diseases (2001)

Orthotopic liver transplantation is the treatment of choice for several inborn errors of metabolism. Unfortunately, the supply of donor organs is limiting and therefore many patients cannot benefit from this therapy. In contrast, hepatocyte transplantation could potentially overcome the shortage in donor livers by use of cells from a single donor for multiple recipients. In classic hepatocyte transplantation, however, only 1% of the liver mass or less can be replaced by donor cells. Recently, though, it has been shown in animal models that >90% of host hepatocytes can be replaced by a small number of transplanted donor cells in a process we term 'therapeutic liver repopulation'. This phenomenon is analogous to repopulation of the haematopoietic system after bone marrow transplantation. Liver repopulation occurs when transplanted cells have a growth advantage in the setting of damage to recipient liver cells. It has been discovered that transplanted cells from extrahepatic sources such as the adult pancreas or bone marrow can also be used for liver repopulation. Because bone marrow donors are widely available, this finding raises the hope of therapeutic application of these cells in the future. Here, the current knowledge regarding therapeutic liver repopulation and the hopeful implications for treatment of liver diseases will be discussed.
The D2 Dopamine Receptor Gene as a Determinant of Reward Deficiency Syndrome (1996)

The dopaminergic system, and in particular the dopamine D2 receptor, has been profoundly implicated in reward mechanisms in the brain. Dysfunction of the D2 dopamine receptors leads to aberrant substance seeking behaviour (alcohol, drug, tobacco, and food) and other related behaviours (pathological gambling, Tourette's syndrome, and attention deficit hyperactivity disorder). We propose that variants of the D2 dopamine receptor gene are important common genetic determinants of the 'reward deficiency syndrome'.
Version 2 of the Reliable Data Protocol (RDP) (1990)

This RFC suggests several updates to the specification of the Reliable
Data Protocol (RDP) in RFC-908 based on experience with the protocol.
This revised version of the protocol is experimental.
Studies of the pathogenesis of anemia of inflammation: Mechanism of impaired erythropoiesis (1983)

Cats with induced sterile abscesses developed a hematologic disorder consistent with anemia of inflammation. Serum iron concentrations decreased while the abscess was present, but erythropoietin concentrations did not change significantly. Cobalt administration to control (healthy) cats resulted in polycythemia, reticulocytosis, and hyperferremia. Cats with abscesses responded to cobalt similarly; however, magnitudes of the polycythemia and reticulocytosis were less. Constant infusion of ferric citrate (IV) into cats with sterile abscesses maintained serum iron concentration in the normal to high range. The iron infusion did not prevent the anemia, but did enable the bone marrow to respond to the anemia.
Visualization of the paranemic joining of homologous DNA molecules catalyzed by the RecA protein of Escherichia coli. (1986)

In reactions catalyzed by the RecA protein of Escherichia coli, synapsis between two DNA molecules is believed to occur even in the absence of free homologous DNA ends and to involve a metastable interaction termed paranemic joining. We have used electron microscopic methods to visualize synapse formation between supertwisted M13 double-stranded DNA (dsDNA) and linear M13 mp7 single-stranded DNA (ssDNA) with non-M13 sequences at its ends. These non-M13 sequences block strand invasion and make this pairing equivalent to the joining of two fully circular molecules. We observed a high frequency of joining when the ssDNA was initially assembled into presynaptic filaments with RecA protein. Cleavage of the dsDNA in the joined complexes by Hpa I revealed that the joint was at a site of homology. In these joints, the dsDNA entered the presynaptic filament over a length of 360 +/- 80 base pairs, not visibly altering its ultrastructure, and then dissociated from the filament. Although the dsDNA in the complexes appeared topologically relaxed, deproteinization released supertwisted dsDNA, indicating that the dsDNA was unwound by 34 degrees per base pair in the paranemic joint. When supertwisted M13 dsDNA was paired with circular M13 ssDNA, similar joints were observed and both DNA circles appeared topologically relaxed.
Targeting HMGB1 in the treatment of sepsis (2014)

It will be important to evaluate the efficacy of HMGB1-targeting strategies for the clinical management of human sepsis in the future.
A near-optimal algorithm for computing the entropy of a stream (2007)

We describe a simple algorithm for approximating the empirical entropy of a stream of m values in a single pass, using O(e-2 log(δ-1) log m) words of space. Our algorithm is based upon a novel extension of a method introduced by Alon, Matias, and Szegedy [1]. We show a space lower bound of Ω(e-2 / log(e-1)), meaning that our algorithm is near-optimal in terms of its dependency on e. This improves over previous work on this problem [8, 13, 17, 5]. We show that generalizing to kth order entropy requires close to linear space for all k ≥ 1, and give additive approximations using our algorithm. Lastly, we show how to compute a multiplicative approximation to the entropy of a random walk on an undirected graph.