gebhart/patrepeval huggingface dataset:
Molotov Cocktails to Mass Marches: Strategic Nonviolence, Symbolic Violence, and the Mobilizing Effect of Riots (2021)

What effects do violent protests have on social movement mobilizations? In recent decades, the field of nonviolence studies has popularized a strategic nonviolence framework to understand activist tactics. This framework is problematic in two ways. First, dominant theories argue that violent protest actions demobilize nonviolent protest. However, there is less empirical support for this claim than often assumed. Current quantitative findings on the demobilizing effects of violent protest rely on a false dichotomy between violence and nonviolence that obscures the effects of low-level violent actions. Through statistical analysis of protest trends in the US over 72 years, I show that riots have an overall mobilizing impact on nonviolent protests. Second, the strategic nonviolence framing encourages an instrumental view of tactics that is prone to miss the symbolic and emotional aspects of different types of actions. Through qualitative interviews with participants in the black bloc tactic, I explore the experiential effects of the riot, and find that rioting can have deeply empowering emotional impacts on participants, with lasting effects that sustain activists’ political engagement. In combination, these results demonstrate that low-level violent actions interact with movements in more dynamic ways than dominant theories have understood. [Article copies available for a fee from The Transformative Studies Institute. E-mail address: journal@transformativestudies.org Website: http://www.transformativestudies.org ©2021 by The Transformative Studies Institute. All rights reserved.]
MMSET deregulation affects cell cycle progression and adhesion regulons in t(4;14) myeloma plasma cells (2008)

In conclusion, dysregulation of MMSET affects the expression of several genes involved in the regulation of cell cycle progression, cell adhesion and survival.
Metal Nitride/Graphene Nanohybrids: General Synthesis and Multifunctional Titanium Nitride/Graphene Electrocatalyst (2011)

A facile, efficient, and general strategy is developed for the fabrication of a new class of nanohybrids consisting of nitrogen-doped graphene functionalized with metal nitride nanoparticles. The graphene decorated with titanium nitride nanoparticles is explored for multifunctional electrocatalytic applications, i.e., as a low-cost counter electrode for I(3)(-) reduction in dye-sensitized solar cells and for nicotinamide adenine dinucleotide (NADH) oxidation in dehydrogenase enzyme-based biosensors.
Immunocontraception with Zona pellucida Proteins (2000)

The prospect of an immunological approach to contraception that would disrupt the process of fertilisation itself has resulted in a considerable interest into research in this area. It has been known for some time that antibodies raised against the zona pellucida (ZP) can suppress fertility very effectively. However, the initial optimism of this approach has been marred by the appearance of an ovarian pathology characterised by disruption of folliculogenesis and depletion of the primordial follicle pool. Adverse auto-immune reactions have been observed in the ovaries of mice after the induction of immunity with mouse ZP3 epitopes. However, this was associated with lymphocytic infiltration of the ovarian stroma, which could be circumvented by careful selection of B-cell epitopes to induce reversible infertility. In order to identify similar epitopes on primate ZP3, epitope-mapping studies were performed and incorporated into chimeric vaccines that included a promiscuous T-helper cell epitope. Both single and triple peptide vaccines have been evaluated in vivo and no detrimental effects on ovarian function were observed. The resulting high titre antibodies bound exclusively to the ZP of marmoset and human ovarian sections and could suppress in vitro human sperm-egg binding by approximately 60%, but did not prevent pregnancy in actively immunised female marmosets. Thus, considerable research is still required to identify a combination of ZP3 epitopes that will induce infertility free of any unwanted side effects.
Linear Models and Empirical Bayes Methods for Assessing Differential Expression in Microarray Experiments (2004)

The problem of identifying differentially expressed genes in designed microarray experiments is considered. Lonnstedt and Speed (2002) derived an expression for the posterior odds of differential expression in a replicated two-color experiment using a simple hierarchical parametric model. The purpose of this paper is to develop the hierarchical model of Lonnstedt and Speed (2002) into a practical approach for general microarray experiments with arbitrary numbers of treatments and RNA samples. The model is reset in the context of general linear models with arbitrary coefficients and contrasts of interest. The approach applies equally well to both single channel and two color microarray experiments. Consistent, closed form estimators are derived for the hyperparameters in the model. The estimators proposed have robust behavior even for small numbers of arrays and allow for incomplete data arising from spot filtering or spot quality weights. The posterior odds statistic is reformulated in terms of a moderated t-statistic in which posterior residual standard deviations are used in place of ordinary standard deviations. The empirical Bayes approach is equivalent to shrinkage of the estimated sample variances towards a pooled estimate, resulting in far more stable inference when the number of arrays is small. The use of moderated t-statistics has the advantage over the posterior odds that the number of hyperparameters which need to estimated is reduced; in particular, knowledge of the non-null prior for the fold changes are not required. The moderated t-statistic is shown to follow a t-distribution with augmented degrees of freedom. The moderated t inferential approach extends to accommodate tests of composite null hypotheses through the use of moderated F-statistics. The performance of the methods is demonstrated in a simulation study. Results are presented for two publicly available data sets.
Enhancement of red blood cell transfusion compatibility using CRISPR‐mediated erythroblast gene editing (2018)

Regular blood transfusion is the cornerstone of care for patients with red blood cell (RBC) disorders such as thalassaemia or sickle-cell disease. With repeated transfusion, alloimmunisation often occurs due to incompatibility at the level of minor blood group antigens. We use CRISPR-mediated genome editing of an immortalised human erythroblast cell line (BEL-A) to generate multiple enucleation competent cell lines deficient in individual blood groups. Edits are combined to generate a single cell line deficient in multiple antigens responsible for the most common transfusion incompatibilities: ABO (Bombay phenotype), Rh (Rh<sub>null</sub>), Kell (<i>K</i><sub>0</sub>), Duffy (Fy<sub>null</sub>), GPB (S-s-U-). These cells can be differentiated to generate deformable reticulocytes, illustrating the capacity for coexistence of multiple rare blood group antigen null phenotypes. This study provides the first proof-of-principle demonstration of combinatorial CRISPR-mediated blood group gene editing to generate customisable or multi-compatible RBCs for diagnostic reagents or recipients with complicated matching requirements.
β‐Adrenergic receptor stimulation of L‐type Ca2+ channels in rabbit portal vein myocytes involves both αs and βγ G protein subunits (2001)

1. Previous studies have shown that purified G protein alphas and betagamma subunits stimulate vascular L-type Ca2+ channels through protein kinase A and C (PKA and PKC), respectively. The present study tested whether activation of endogenous G proteins via beta-adrenergic receptor binding also stimulates vascular Ca2+ channels through both Galphas and Gbetagamma and the subsequent activation of PKA and PKC. 2. Peak Ba2+ current (IBa) in freshly isolated rabbit portal vein smooth muscle cells was significantly increased by bath application of 0.5 microM isoproterenol (isoprenaline; ISO) when measured using the whole-cell patch clamp method (53 +/- 3 % increase, n = 15). Stimulation of IBa by ISO was partially reversed by a PKA inhibitor, KT 5720, or a PKC inhibitor, calphostin C, and completely blocked when cells were pretreated with both KT 5720 and calphostin C. 3. Dialysis of cells with polyclonal antibody to Galphas significantly reduced but did not completely eliminate ISO-induced stimulation of IBa. The remaining stimulation was abolished by calphostin C. Dialysis of cells with a polyclonal antibody to Gbeta also significantly reduced ISO-induced stimulation and the remaining stimulation was abolished by KT 5720. Dialysis of cells with both antibodies completely prevented the stimulation of IBa by ISO. 4. ISO-induced stimulation of IBa was reversed by ICI-118,551, a specific beta2-adrenoceptor antagonist, but not by CGP 20712A, a specific beta1-adrenoceptor antagonist. In addition, the beta2-adrenoceptor agonist zinterol significantly increased peak IBa while the beta1-adrenoceptor agonist dobutamine and beta3-adrenoceptor agonist BRL 37344A had little effect on peak IBa. 5. These data suggest that beta-adrenergic receptor stimulation of vascular L-type Ca2+ channels involves both alphas and betagamma G-protein subunits, which exert their effects through PKA and PKC, respectively.
Cell characterization using a protein-functionalized pore (2008)

We demonstrate a highly-sensitive and label-free method for characterizing cells based on cell-surface receptors. The method involves measuring a current pulse generated when an individual cell passes through an artificial pore. When the pore is functionalized with proteins, specific interactions between a cell-surface marker and the functionalized proteins retard the cell, thus leading to an increased pulse duration that indicates the presence of that specific biomarker. For proof-of-principle, we successfully screened murine erythroleukemia cells based on their CD34 surface marker in both a single and mixed population of cells. Further, we developed a unified constrained statistical model for estimating the ratios of cells in a mixed population. Finally, we demonstrated our ability to screen a small number of cells (hundreds or less) with high accuracy and sensitivity. Overall, our pore-based method is broadly applicable and, in the future, could provide a full range of in vitro cell-based assays.
A novel promoter controls Cyp19a1 gene expression in mouse adipose tissue (2009)

Taken together, this research identified a novel, adipose-specific first exon of Cyp19a1 and its hormonally regulated promoter region in male murine gonadal fat. These results expand the known 5'-regulatory region of the murine Cyp19a1 gene to 75 kb upstream of the translation start site. Cyp19a1 expression in mouse adipose tissue may play an important role in reproductive biology and lipid metabolism.
Word Co-occurrence Augmented Topic Model in Short Text (2015)

The large amount of text on the Internet cause people hard to understand the meaning in a short limit time. Topic models (e.g. LDA and PLSA) has been proposed to summarize the long text into several topic terms. In the recent years, the short text media such as tweet is very popular. However, directly applies the transitional topic model on the short text corpus usually gating non-coherent topics. Because there is no enough words to discover the word co-occurrence pattern in a short document. The Bi-term topic model (BTM) has been proposed to improve this problem. However, BTM just consider simple bi-term frequency which cause the generated topics are dominated by common words. In this paper, we solve the problem of the frequent bi-term in BTM. Thus, we proposed an improvement of word co-occurrence method to enhance the topic models. We apply the word co-occurrence information to the BTM. The experimental result that show our PMI-β-BTM gets well result in the both of regular short news title text and the noisy tweet text. Moreover, there are two advantages in our method. We do not need any external data and our proposed methods are based on the original topic model that we did not modify the model itself, thus our methods can easily apply to some other existing BTM based models.
Lignin modification by epoxidation (2008)

The chemical modification of lignin through hydroxymethylation and epoxidation is a way to develop new application fields and improvement of lignin performances. In this paper the influence of reaction conditions was studied using different samples of unmodified and modified lignins from annual plants (Wheat straw and Sarkanda grass). The progress of reactions was monitored by evaluating the epoxy index. The resulting products were characterized by FTIR-spectra, UV-Vis spectroscopy, and thermogravimetry analysis. The products obtained were tested to be used in composite formulation for wood applications.
Predicting sequence-dependent melting stability of short duplex DNA oligomers (1997)

Many important applications of DNA sequence-dependent hybridization reactions have recently emerged. This has sparked a renewed interest in analytical calculations of sequence-dependent melting stability of duplex DNA. In particular, for many applications it is often desirable to accurately predict the transition temperature, or tm of short duplex DNA oligomers (approximately 20 base pairs or less) from their sequence and concentration. The thermodynamic analytical method underlying these predictive calculations is based on the nearest-neighbor model. At least 11 sets of nearest-neighbor sequence-dependent thermodynamic parameters for DNA have been published. These sets are compared. Use of the nearest-neighbor sets in predicting tm from the DNA sequence is demonstrated, and the ability of the nearest-neighbor parameters to provide accurate predictions of experimental tm's of short duplex DNA oligomers is assessed.
Bisphosphonates – A Word of Caution (2004)

There have been recent reports from various centres of a possible association between avascular necrosis of the jaws and the use of bisphosphonates. This commentary reviews the available data and alerts clinicians to a possible adverse reaction in the oral cavity to a group of drugs that are commonly prescribed.
Silicon-on-quartz for low power electronic applications (2002)

We have developed a novel Silicon-on-Quartz (SOQ) technology for fabricating SOI devices and circuits for low power electronics applications. SOQ substrate is fabricated by direct bonding of a silicon wafer with an etch-stop layer, and a quartz wafer, at room temperature, followed by thinning using surface grinding and selective etching techniques. We have successfully fabricated SOQ CMOS devices and demonstrated transistors with high mobility and low leakage currents. Thermal stress management techniques employed for the successful fabrication of the SOQ CMOS devices and the advantages of the SOQ approach for low power electronics are discussed.
The translational potential for target validation and therapy using intracellular antibodies in oncology. (2014)

Antibody-based molecules can be delivered into cells either by intracellular expression or through cellular uptake. We describe technologies for identification and expression of intracellular antibodies for target validation, intracellular immunization and tumor therapy, such as intracellular antibody capture technology, suicide or silencing technology, antigen-antibody interaction dependent apoptosis and their application for inhibition of oncogenic intracellular proteins and induction of apoptosis. These strategies have to be viewed in the context that inhibition of protein-protein interactions by small molecules is often limited due to their large interaction surface. We summarize antibodies with the ability to penetrate cells and strategies to induce uptake of antibodies after modification with protein transduction domains. Interference in oncogenic pathways is described for moieties based on antibody 3E10, which translocates into the nucleus after extracellular administration. Finally, we discuss examples of tumor immunotherapy and vaccination against intracellular antigens, and possible interactions mediating their mode of action.
Standardization of <i>Dermatophagoides pteronyssinus:</i> Assessment of Potency and Allergen Content in Ten Coded Extracts (1985)

As part of the studies to establish an international reference preparation of Dermatophagoides pteronyssinus allergens, ten coded extracts of this mite were assessed in four laboratories. The extracts were compared for total potency using direct RAST, RAST inhibition and quantitative skin tests, and also for composition and major allergen content using crossed immunoelectrophoresis, crossed radioimmunoelectrophoresis, rocket immunoelectrophoresis and radioimmunoassay. In addition, the source materials were examined by light microscopy, and the extracts were examined for the presence of proteins/allergens derived from the culture media. To help with standardization, a new reference pool of sera from patients allergic to D. pteronyssinus was also established (National Institute of Biological Standards and Control, NIBSC, 82/528). The results showed that techniques are available for measurements of potency and allergen content. In several of the extracts, culture medium derived allergens and antigens were demonstrated. It also became clear that extracts varied not only in their total potency but also in the distribution of the identifiable allergens. In particular, extracts derived from isolated mites contained more AgX and/or Ag 23 relative to their content of antigen P1 (= Ag42). These studies lead to the choice of an extract for an international reference preparation (NIBSC 82/518) and helped to establish the methods used subsequently in the international collaborative study.
Spatial assessment of articular cartilage proteoglycans with Gd‐DTPA‐enhanced T1 imaging (2002)

In Gd-DTPA-enhanced T(1) imaging of articular cartilage, the MRI contrast agent with two negative charges is understood to accumulate in tissue inversely to the negative charge of cartilage glycosaminoglycans (GAGs) of proteoglycans (PGs), and this leads to a decrease in the T(1) relaxation time of tissue relative to the charge in tissue. By assuming a constant relaxivity for Gd-DTPA in cartilage, it has further been hypothesized that the contrast agent concentration in tissue could be estimated from consecutive T(1) measurements in the absence or presence of the contrast agent. The spatial sensitivity of the technique was examined at 9.4 T in normal and PG-depleted bovine patellar cartilage samples. As a reference, spatial PG concentration was assessed with digital densitometry from safranin O-stained cartilage sections. An excellent linear correlation between spatial optical density (OD) of stained GAGs and T(1) with Gd-DTPA was observed in the control and chondroitinase ABC-treated cartilage specimens, and the MR parameter accounted for approximately 80% of the variations in GAG concentration within samples. Further, the MR-resolved Gd-DTPA concentration proved to be an even better estimate for PGs, with an improved correlation. However, the linear relation between MR parameters and PG concentration did not apply in the deep tissue, where MR measurements overestimated the PG content. While the absolute [Gd-DTPA] determination may be prone to error due to uncertainty of relaxivity in cartilage, or to other contributing factors such as variations in tissue permeability, the experimental evidence highlights the sensitivity of this technique to reflect spatial changes in cartilage PG concentration in normal and degenerated tissue.
Synthesis of open metal carbonyl cluster compounds. The reactions of closo-sulfido metal carbonyl cluster compounds with hydrogen sulfide. Synthesis and crystal and molecular structure of H2Os5(CO)14(.mu.3-S)2 (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of open metal carbonyl cluster compounds. The reactions of closo-sulfido metal carbonyl cluster compounds with hydrogen sulfide. Synthesis and crystal and molecular structure of H2Os5(CO)14(.mu.3-S)2Richard D. Adams, Istvan T. Horvath, and Li Wu YangCite this: Organometallics 1983, 2, 9, 1257–1258Publication Date (Print):September 1, 1983Publication History Published online1 May 2002Published inissue 1 September 1983https://pubs.acs.org/doi/10.1021/om50003a036https://doi.org/10.1021/om50003a036research-articleACS PublicationsRequest reuse permissionsArticle Views93Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Global measures of coherence for edge detector evaluation (2003)

We propose a class of benchmarks for edge detector evaluation that require no ground truth. Each benchmark consists of a large number of images of a carefully designed scene for which we enforce a constraint on the edges, for example, that they are co-linear. We sample the space of edge appearances as densely as possible by capturing the images under widely varying imaging conditions. Not only do we change the viewing geometry and the illumination direction, but we also vary the camera parameters and the physical properties of the objects in the scene. We show that the degrees to which the constraints hold in the output edge-maps can be used as highly discriminating measures of edge detector performance. The code, images, and results which form our benchmarks are all available from the website http://www.cs.columbia.edu/CAVE/. The code and images enable a user to compare any new detector against several previous ones with minimal effort.