gebhart/patrepeval huggingface dataset:
A carrier injection type optical switch in GaAs using free carrier plasma dispersion with wavelength range from 1.06 to 1.55 mu m (1989)

The switching current for the device is about 90 mA at all in-range wavelengths. Its broad wavelength range is suitable for optical signal-processing applications in wavelength-division multiplexing transmission systems. High coupling efficiency to a single-mode fiber is achieved by using a small- Delta waveguide structure with carrier blocking layers. The effective refractive index change in the waveguide per unit of the injected current density is doubled by the carrier blocking layers without reducing coupling efficiency.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Artificial Neural Networks Analysis of Surface-Enhanced Laser Desorption/Ionization Mass Spectra of Serum Protein Pattern Distinguishes Colorectal Cancer from Healthy Population (2004)

The combination of SELDI-TOF mass spectrometry with the artificial neural networks in the analysis of serum protein yields significantly higher sensitivity and specificity values for the detection and diagnosis of colorectal cancer.
Neutralization of adenoviruses: kinetics, stoichiometry, and mechanisms (1988)

Kinetic curves for neutralization of adenovirus type 2 with anti-hexon serum revealed no lag periods even when the serum was highly diluted or when the temperature was lowered to 4 degrees C, thus indicating a single-hit mechanism. Multiplicity curves determined with anti-hexon serum displayed a linear correlation between the degree of neutralization and dilution of antiserum. Neutralization values experimentally obtained under steady-state conditions fully fitted a single-hit model based on Poisson calculations. Quantitation of the amount of 125I-labeled type-specific anti-hexon antibodies needed for full neutralization of adenovirus showed that 1.4 antibodies were attached per virion under such conditions. Virions already attached to HeLa cells at 4 degrees C were, to a large extent, neutralizable by anti-hexon serum, whereas anti-fiber and anti-penton base antisera were negative. It is suggested that adenovirus may be neutralized by two pathways: aggregation of the virions (extracellular neutralization) as performed by anti-fiber antibodies and blocking of virion entrance from the acidic endosomes into the cytoplasm (intracellular neutralization). The latter effect could be obtained by (i) covering of the penton bases, as performed by anti-penton base antibodies, thereby preventing interaction between the penton bases and the endosomal membrane, which results in trapping of virions within endosomes, and (ii) inhibition of the low-pH-induced conformational change of the viral capsid, which seems to occur in the endosomes and is necessary for proper exposure of the penton bases, as performed by anti-hexon antibodies.
Prediction of pseudophakic capsular bag diameter based on biometric variables12 (1999)

The capsular bag diameter in vivo correlated negatively with corneal power (P) and positively with AL. The regression formula used may serve as a tool for preoperative identification of eyes with a very large or very small CBDm.
A novel developmentally regulated gene in lung mesenchyme: homology to a tumor-derived trypsin inhibitor (1999)

We used differential display-PCR (DD-PCR) to identify glucocorticoid-inducible genes that regulate lung development in late gestation. DD-PCR, a method to screen for differentially expressed genes, is based on a comparison of mRNAs isolated from a subset of two or more cell populations by analysis of RT-PCR products on DNA-sequencing gels. We isolated cDNA probes representing mRNAs expressed in primary cultures of rat lung fibroblasts, but not in epithelial cells, on fetal day 20. A day 20 glucocorticoid-treated fibroblast cDNA library was screened with a single probe to isolate the 3.1-kb cDNA late-gestation lung 1 (LGL1; GenBank accession no. AF109674) encoding a deduced polypeptide of 188 amino acids. Northern analysis confirmed that LGL1 is expressed in human, rat, and mouse fetal lungs, induced by glucocorticoid, developmentally regulated in fibroblasts but not detectable in epithelium. In situ hybridization confirmed LGL1 expression in the mesenchyme, but not in the epithelium, of fetal rat lung, kidney, and gut. The predicted LGL1 gene product (lgl1) showed 81% homology to P25TI, a polypeptide trypsin inhibitor recently identified in human glioblastoma and neuroblastoma cells but not detected in normal human tissues. Both lgl1 and P25TI belong to the CRISP family of cysteine-rich extracellular proteins. Trypsin is produced by both normal bronchial epithelial and lung adenocarcinoma cells. Although additional studies will be necessary to clearly establish a functional role for lgl1, we propose that lgl1 has a role in normal lung development that is likely to be via regulation of extracellular matrix degradation.
Tropisms of AAV for Subretinal Delivery to the Neonatal Mouse Retina and Its Application for In Vivo Rescue of Developmental Photoreceptor Disorders (2013)

In the current study, we report suitable AAV tropisms for delivery into the developing mouse retina. Using AAV2/5 in photoreceptor cells, we demonstrated the possibility of gene replacement for the developmental disorder and subsequent degeneration of retinal photoreceptors caused by the absence of Crx.
Compact Nonlinear Model of an Implantable Electrode Array for Spinal Cord Stimulation (SCS) (2013)

We describe the construction of a model of the electrode-electrolyte interface and surrounding electrolyte in the case of a platinum-electrode array intended for spinal-cord stimulation (SCS) application. We show that a finite, two-dimensional, resistor array provides a satisfactory model of the bulk electrolyte, and we identify the complexity required of that resistor array. The electrode-electrolyte interface is modelled in a fashion suitable for commonly-available, compact simulators using a nonlinear extension of the model of Franks (IEEE Trans. Biomed. Eng., vol. 52 , no. 7 , pp. 1295-1302, Jul. 2005) that incorporates diodes and a memristor. The electrode-electrolyte interface model accounts for the nonlinear current-overpotential characteristic and diffusion-limiting effects. We characterise a commercial, implantable, electrode array, fit the model to it, and show that the model successfully predicts subtle operational characteristics.
Insights into the action of the superfamily of cholesterol-dependent cytolysins from studies of intermedilysin (2005)

The cholesterol-dependent cytolysins (CDCs), a superfamily of pore-forming toxins, are characterized by a conserved undecapeptide motif that is believed to be critical for membrane recognition by means of cholesterol. Intermedilysin (ILY), an unusual member of the CDCs, exhibits specificity for human cells and contains nonconservative substitutions in the motif. We show that the cellular specificity of ILY is based on its ability to specifically bind to human cells and does not involve some other feature of the CDC mechanism. Furthermore, cellular recognition by ILY appears to be encoded in domain 4 alone but does not involve the variant undecapeptide of ILY. We show that the undecapeptide is involved in the prepore-to-pore conversion of ILY and so demonstrate a direct connection between the structure of the undecapeptide and the prepore-to-pore transition. We have determined the crystal structure of ILY, which, when compared to the known structure of a prototypical CDC, suggests that the basic aspects of its 3D structure are likely to be conserved in all CDCs.
Biosensor Based on Cellobiose Dehydrogenase for Detection of Catecholamines (2004)

A cellobiose dehydrogenase (CDH)-modified graphite electrode was designed for amperometric detection of catecholamines in the flow injection mode, by their recycling between the graphite electrode (+300 mV vs Ag|AgCl) and the reduced FAD cofactor of adsorbed CDH, resulting in an amplified response signal. The high efficiency of the enzyme-catecholamine reaction leads to a detection limit below 1 nM and a sensitivity of 15.8 A.M(-1) x cm(-2) (approximately 1150 nA/microM) for noradrenaline, with a coverage of less than 2.5 microg of CDH adsorbed on the electrode surface (0.073 cm(2)). Working parameters such as pH, cellobiose concentration, carrier buffer, and applied potential were optimized, using hydroquinone as a model analyte. The sensitivity, linear range, and amplification factor can be modulated by the steady-state concentration of cellobiose in the flow buffer. The response of the sensor decreases only 2% when run continuously for 4 h in the flow injection mode. The response peak maximum is obtained within 6 s at a flow rate of 0.5 mL/min, representing the time of the entire sample segment to pass the electrode. CDH enzymes from Phanerochaete chrysosporium and Sclerotium rolfsii were investigated, providing different characteristics of the sensor, with sensors made with CDH from P. chrysosporium being the better ones.
High-resolution, high-speed microscanner in single-crystalline silicon actuated by self-aligned dual-mode vertical electrostatic combdrive with capability for phased array operation (2004)

We present micromirrors actuated by self-aligned, high aspect ratio, vertical electrostatic combdrives with multi-level electrical isolation that allows bi-directional and dual-mode (independent rotation and piston motion) operation. The fabrication process is based on self-aligned DRIE of SOI wafers with two device layers. The two oxide layers provide electrode isolation and etch stops for thickness control. Only front-side processing is required. We demonstrate micromirrors of this type with /spl plusmn/9 degrees of optical scanning and 7.5 /spl mu/m of piston motion under static actuation. Micromirrors designed for resonant scanning have optical scan angles up to /spl plusmn/25 degrees at 13.5 kHz.
Stem cell technology and bioceramics: From cell to gene engineering (1999)

Mesenchymal stem cells reside in bone marrow and, when these cells are incorporated into porous ceramics, the composites exhibit osteo-chondrogenic phenotypic expression in ectopic (subcutaneous and intramuscular) or orthotopic sites. The expressional cascade is dependent upon the material properties of the delivery vehicle. Bioactive ceramics provide a suitable substrate for the attachment of the cells. This is followed by osteogenic differentiation directly on the surface of the ceramic, which results in bone bonding. Nonbioactive materials show neither surface-dependent cell differentiation nor bone bonding. The number of mesenchymal stem cells in fresh adult bone marrow is small, about one per one-hundred-thousand nucleated cells, and decreases with donor age. In vitro cell culture technology can be used to mitotically expand these cells without the loss of their developmental potency regardless of donor age. The implanted composite of porous ceramic and culture-expanded mesenchymal stem cells exhibits in vivo osteo-chondrogenic differentiation. In certain culture conditions, these stem cells differentiate into osteoblasts, which make bone matrix on the ceramic surface. Such in vitro prefabricated bone within the ceramic provides immediate new bone-forming capability after in vivo implantation. Prior to loading of the cultured, marrow-derived mesenchymal stem cells into the porous ceramics, exogenous genes can be introduced into these cells in culture. Combining in vitro manipulated mesenchymal stem cells with porous ceramics can be expected to effect sufficient new bone-forming capability, which can thereby provide tissue engineering approaches to patients with skeletal defects in order to regenerate skeletal tissues.
Reactions on Polymer Supports (1964)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReactions on Polymer SupportsR. L. Letsinger, M. J. Kornet, V. Mahadevan, and D. M. JerinaCite this: J. Am. Chem. Soc. 1964, 86, 23, 5163–5165Publication Date (Print):December 1, 1964Publication History Published online1 May 2002Published inissue 1 December 1964https://pubs.acs.org/doi/10.1021/ja01077a024https://doi.org/10.1021/ja01077a024research-articleACS PublicationsRequest reuse permissionsArticle Views442Altmetric-Citations113LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Altered Expression of Neurotensin Receptors Is Associated with the Differentiation State of Prostate Cancer (2010)

In prostate cancer, traditional treatments such as androgen response manipulation often provide only temporary resolution of disease, with emergence of a more aggressive, androgen-independent tumor following initial therapy. To treat recurrent disease, cell surface proteins that are specifically overexpressed on malignant cells may be useful for generating targeted therapeutics. Recent evidence suggests that neurotensin receptors (NTR) are recruited in advanced prostate cancer as an alternative growth pathway in the absence of androgens. In this study, we assessed the potential use of these receptors as targets by analyzing NTR expression patterns in human prostate cell lines and primary prostate tumor cell cultures derived from patient samples. In primary tumor cell cultures, NTR1 was upregulated in cells with a basal phenotype (cytokeratin 1/5/10/14+), whereas NTR2 and NTR3 were upregulated in cells with luminal phenotype (cytokeratin 18+). Similar patterns of NTR expression occurred in benign prostate tissue sections, implicating differentiation state as a basis for the differences observed in tumor cell lines. Our findings support the use of NTRs as tools for therapeutic targeting in prostate cancers composed of both poorly differentiated and/or well-differentiated cells.
Annealing influence on the Gilbert damping parameter and the exchange constant of CoFeB thin films (2014)

We present a study of the influence of the annealing temperature Ta on the magnetic properties of Co40Fe40B20 thin films. Using a vector network analyzer ferromagnetic resonance (VNA-FMR) setup and the magneto-optical Kerr effect, the dependence of the Gilbert damping parameter α, the exchange constant A, the saturation magnetization as well as the coercive field and the Kerr signal on Ta is reported. Additionally, the correlation with the crystalline properties of the films studied by X-ray diffractometry is discussed. We found that while the damping parameter α and the coercive field show sharp changes starting at a certain Ta value, the exchange constant A and the Kerr signal show a steady evolution. A differential modification of the film surface compared to the bulk is discussed as a possible reason. In any case, we found that the low damping values are preserved at the first onset of crystallization.
Detecting Hg2+ Ions with an ICT Fluorescent Sensor Molecule:  Remarkable Emission Spectra Shift and Unique Selectivity (2006)

A fluorescent ratiometric Hg2+ ion sensor RMS, based on a coumarin platform coupled with a tetraamide receptor, is presented. This sensor, employing the ICT mechanism, could be used to specifically detect Hg2+ ions in a neutral buffered water solution with an approximately 100-nm blue shift in emission spectra.
Review of naltrexone, a long-acting opiate antagonist. (1984)

The basis for using narcotic antagonists for the treatment of opiate addiction is discussed briefly, and the chemistry, pharmacology, pharmacokinetics, clinical efficacy, adverse effects, and dosage of naltrexone hydrochloride, an opiate antagonist drug, are reviewed. Naltrexone is rapidly and completely absorbed after oral administration but undergoes substantial first-pass extraction and metabolism by the liver. Naltrexone has a half-life of 3.9-10.3 hours and a slow terminal elimination-phase half-life of 96 hours. The major metabolite of naltrexone is 6-beta-naltrexol, which is present in plasma in greater concentrations than the parent drug. Problems in study design and patient adherence to treatment have made assessment of naltrexone's clinical efficacy difficult; however, studies have consistently demonstrated that patients who remained off opiates longer were those who took naltrexone longer. Factors associated with successful treatment outcomes include sustained therapy with naltrexone, participation in multidisciplinary programs of behavioral therapy and psychotherapy, and good family and social support systems. Gastrointestinal irritation and, in some studies, clinically insignificant increases in blood pressure, have been the most common adverse effects. Currently available data suggest that naltrexone is a very safe drug. The usual dosage of naltrexone hydrochloride is 50 mg orally once daily or 350 mg orally per week in three divided doses. Patients should be detoxified and opiate free for two to five days before initiation of naltrexone therapy. Naltrexone appears to be a useful adjunct to therapy in opiate addicts who are well motivated and who have strong psychological support systems.
XML Document Analysis based on Similarity (2002)

XML allows users to define elements using arbitrary words and organize them in a nested structure. These features of XML offer both challenges and opportunities in information retrieval and document management. In this paper, we propose a new methodology for computing similarity considering XML semantics - meanings of the elements and nested structures of XML documents. We generate extended-element vectors, using thesaurus, to normalize synonyms, compound words, and abbreviations and build similarity matrix using them. And then we compute similarity between XML elements. We also discover and minimize XML structure using automata(NFA(Nondeterministic Finite Automata) and DFA(Deterministic Finite automata). We compute similarity between XML structures using similarity matrix between elements and minimized XML structures. Our methodology considering XML semantics shows 100% accuracy in identifying the category of real documents from on-line bookstore.
Inhibitor-Sensitive FGFR1 Amplification in Human Non-Small Cell Lung Cancer (2011)

These studies show that FGFR1 amplification is common in squamous cell lung cancer, and that FGFR1 may represent a promising therapeutic target in non-small cell lung cancer.
High Molecular Weight Polymethacrylates by AGET ATRP under High Pressure (2008)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTHigh Molecular Weight Polymethacrylates by AGET ATRP under High PressurePiotr Kwiatkowski, Janusz Jurczak, Joanna Pietrasik, Wojciech Jakubowski, Laura Mueller, and Krzysztof MatyjaszewskiView Author Information Institute of Organic Chemistry, Polish Academy of Sciences, ul Kasprzaka 44/52, 01-224 Warsaw, Poland; Institute of Polymer and Dye Technology, Technical University of Łódź , ul Stefanowskiego 12/16, 90-924 Łódź, Poland; and Center for Macromolecular Engineering, Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213 Cite this: Macromolecules 2008, 41, 4, 1067–1069Publication Date (Web):January 25, 2008Publication History Received12 December 2007Revised14 January 2008Published online25 January 2008Published inissue 1 February 2008https://pubs.acs.org/doi/10.1021/ma702770uhttps://doi.org/10.1021/ma702770urapid-communicationACS PublicationsCopyright © 2008 American Chemical SocietyRequest reuse permissionsArticle Views2517Altmetric-Citations134LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Kinetic parameters,Monomers,Organic compounds,Polymerization,Radical polymerization Get e-Alerts
A new GFP-tagged line reveals unexpected Otx2 protein localization in retinal photoreceptors (2007)

Abstract Background Dynamic monitoring of protein expression and localization is fundamental to the understanding of biological processes. The paired-class homeodomain-containing transcription factor Otx2 is essential for normal head and brain development in vertebrates. Recent conditional knockout studies have pointed to multiple roles of this protein during late development and post-natal life. Yet, later expression and functions remain poorly characterized as specific reagents to detect the protein at any stage of development are still missing. Results We generated a new mouse line harbouring an insertion of the GFP gene within the Otx2 coding sequence to monitor the gene activity while preserving most of its functions. Our results demonstrate that this line represents a convenient tool to capture the dynamics of Otx2 gene expression from early embryonic stages to adulthood. In addition, we could visualize the intracellular location of Otx2 protein. In the retina, we reinterpret the former view of protein distribution and show a further level of regulation of intranuclear protein localization, which depends on the cell type. Conclusion The GFP-tagged Otx2 mouse line fully recapitulates previously known expression patterns and brings additional accuracy and easiness of detection of Otx2 gene activity. This opens up the way to live imaging of a highly dynamic actor of brain development and can be adapted to any mutant background to probe for genetic interaction between Otx2 and the mutated gene.
Nucleotide sequence of the cellobiohydrolase gene fromTrichoderma viride (1990)

Journal Article Nucleotide sequence of the cellobiohydrolase gene from Trichoderma viride Get access Cheng Cheng, Cheng Cheng Faculty of Agriculture, Nagoya UniversityChikusa, Nagoya 464, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Norihiro Tsukagoshi, Norihiro Tsukagoshi Faculty of Agriculture, Nagoya UniversityChikusa, Nagoya 464, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Shigezo Udaka Shigezo Udaka * Faculty of Agriculture, Nagoya UniversityChikusa, Nagoya 464, Japan * To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 18, Issue 18, 25 September 1990, Page 5559, https://doi.org/10.1093/nar/18.18.5559 Published: 25 September 1990 Article history Received: 09 August 1990 Published: 25 September 1990