gebhart/patrepeval huggingface dataset:
TRANSALDOLASE: THE FORMATION OF FRUCTOSE-6-PHOSPHATE FROM SEDOHEPTULOSE-7-PHOSPHATE (1953)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTRANSALDOLASE: THE FORMATION OF FRUCTOSE-6-PHOSPHATE FROM SEDOHEPTULOSE-7-PHOSPHATEB. L. Horecker and P. Z. SmyrniotisCite this: J. Am. Chem. Soc. 1953, 75, 8, 2021–2022Publication Date (Print):April 1, 1953Publication History Published online1 May 2002Published inissue 1 April 1953https://doi.org/10.1021/ja01104a532RIGHTS & PERMISSIONSArticle Views164Altmetric-Citations59LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (266 KB) Get e-Alerts Get e-Alerts
A novel current scaling active pixel sensor with correlated double sampling readout circuit for real time medical x-ray imaging (2007)

This paper presents a new current-programmed, current-output active pixel sensor (APS) based on a novel current scaling scheme and suitable for real time X-ray imaging (fluoroscopy) and a current mode CMOS readout circuit. The circuit is designed using hydrogenated amorphous silicon (a-Si:H) thin film transistor (TFT) technology. The simulation results based on the measured characteristics of a-Si:H TFTs show that the proposed pixel circuit can successfully scale up the current data during readout and therefore provide higher gain compared with the conventional current mode APS circuits. The design can also compensate for characteristic variations (e.g. mobility and threshold voltage shift) in a-Si:H TFTs under prolonged gate voltage stress. The readout circuit exploits correlated double sampling (CDS) technique to reduce the offset current, low frequency noise and fixed-pattern noise (FPN) on the array operation.
Coronary Artery Calcification Is an Independent Stroke Predictor in the General Population (2013)

CAC is an independent stroke predictor in addition to classical risk factors in subjects at low or intermediate vascular risk.
Project Managing HealthCare 2000 (1996)

Successful projects do not just happen - they have to be carefully orchestrated. This paper tells the story of the HealthCare 2000 project from the project manager's perspective. It follows the project from its conception, through its planning and development to its current status today, more than halfway through the project. In hindsight, we are able to reflect on the factors that contribute or detract from the success of a project, and at little more than the halfway mark, ensure that the project comes to a successful completion.
Seroprevalence of GB virus C and persistence of RNA and antibody (1997)

Exposure to GB virus C (GBV-C) was determined in several U.S. populations by both reverse-transcription-polymerase chain reaction (RT-PCR) and by an enzyme linked immunosorbent assay (ELISA) for antibodies to mammalian cell-expressed GBV-C envelope protein, E2 (GBV-C E2). Most individuals exposed to GBV-C were either RNA positive/ELISA negative or ELISA positive/RNA negative. Exposure, therefore, was measured as the sum of GBV-C RNA positive and GBV-C E2 antibody positive specimens, and was higher in commercial plasmapheresis donors (40.5%) than in volunteer blood donors (5.5%). In intravenous drug users (IVDUs), GBV-C exposure was 89.2%. Serial bleed specimens tested for GBV-C RNA indicate that some patients remain viremic for at least 3 years and fail to produce detectable antibodies to GBV-C E2. In other exposed individuals who tested negative for GBV-C RNA, antibodies to E2 appear to be similarly long-lived (greater than 3 years) with a fairly constant titer (ranging in reciprocal endpoint dilution from 336 to 21,504). Since the detection of GBV-C RNA and GBV-C E2 antibody are mutually exclusive in most exposed individuals, studies pertaining to incidence and prevalence of GBV-C infection require both antibody and nucleic acid detection.
Transition of Plasmodium Sporozoites into Liver Stage-Like Forms Is Regulated by the RNA Binding Protein Pumilio (2011)

Many eukaryotic developmental and cell fate decisions that are effected post-transcriptionally involve RNA binding proteins as regulators of translation of key mRNAs. In malaria parasites (Plasmodium spp.), the development of round, non-motile and replicating exo-erythrocytic liver stage forms from slender, motile and cell-cycle arrested sporozoites is believed to depend on environmental changes experienced during the transmission of the parasite from the mosquito vector to the vertebrate host. Here we identify a Plasmodium member of the RNA binding protein family PUF as a key regulator of this transformation. In the absence of Pumilio-2 (Puf2) sporozoites initiate EEF development inside mosquito salivary glands independently of the normal transmission-associated environmental cues. Puf2- sporozoites exhibit genome-wide transcriptional changes that result in loss of gliding motility, cell traversal ability and reduction in infectivity, and, moreover, trigger metamorphosis typical of early Plasmodium intra-hepatic development. These data demonstrate that Puf2 is a key player in regulating sporozoite developmental control, and imply that transformation of salivary gland-resident sporozoites into liver stage-like parasites is regulated by a post-transcriptional mechanism.
Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation (2007)

Recent advances in understanding the cellular and molecular signaling pathways and global transcriptional regulators of adult mesenchymal stem cells have provided new insights into their biology and potential clinical applications, particularly for tissue repair and regeneration. This review focuses on these advances, specifically in the context of self-renewal and regulation of lineagespecific differentiation of mesenchymal stem cells. In addition we review recent research on the concept of stem cell niche, and its relevance to adult mesenchymal stem cells.
Preeclampsia is Associated with Lower Percentages of Regulatory T Cells in Maternal Blood (2009)

We conclude that a deficiency of regulatory T cells may play a role in the pathophysiology of preeclampsia.
Multiplexed diode-laser sensor system for simultaneous H_2O, O_2, and temperature measurements (1994)

A multiplexed diode-laser sensor system has been developed to monitor multiple species and measure multiple flowfield parameters along a single path with optical fibers and scanned-laser absorption techniques. Two InGaAsP lasers were tuned over H2O transitions near 1347 and 1392 nm, and an AlGaAs laser was tuned over an O2 transition near 760.65 nm to record fully resolved absorption concurrently at a repetition rate of 2 kHz. The system was applied to measure H2O, O2, and temperature simultaneously in a heated static cell and in room air. Wavelength modulation with second-harmonic detection, applied simultaneously in the O2 measurements, yielded a minimum detectable absorbance of 1.6 × 10−6/Hz1/2 and an O2 detectivity of 6.3 parts in 106 in a 1-m path length.
Adaptive control of a class of nonlinear discrete-time systems using neural networks (1995)

Layered neural networks are used in a nonlinear self-tuning adaptive control problem. The plant is an unknown feedback-linearizable discrete-time system, represented by an input-output model. To derive the linearizing-stabilizing feedback control, a (possibly nonminimal) state-space model of the plant is obtained. This model is used to define the zero dynamics, which are assumed to be stable, i.e., the system is assumed to be minimum phase. A linearizing feedback control is derived in terms of some unknown nonlinear functions. A layered neural network is used to model the unknown system and generate the feedback control. Based on the error between the plant output and the model output, the weights of the neural network are updated. A local convergence result is given. The result says that, for any bounded initial conditions of the plant, if the neural network model contains enough number of nonlinear hidden neurons and if the initial guess of the network weights is sufficiently close to the correct weights, then the tracking error between the plant output and the reference command will converge to a bounded ball, whose size is determined by a dead-zone nonlinearity. Computer simulations verify the theoretical result.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Wnt10b Inhibits Obesity in ob/ob and Agouti Mice (2007)

The Wnt family of secreted signaling molecules has profound effects on diverse developmental processes, including the fate of mesenchymal progenitors. While activation of Wnt signaling blocks adipogenesis, inhibition of endogenous Wnt/beta-catenin signaling by Wnt10b promotes spontaneous preadipocyte differentiation. Transgenic mice with expression of Wnt10b from the FABP4 promoter (FABP4-Wnt10b) have less adipose tissue when maintained on a normal chow diet and are resistant to diet-induced obesity. Here we demonstrate that FABP4-Wnt10b mice largely avert weight gain and metabolic abnormalities associated with genetic obesity. FABP4-Wnt10b mice do not gain significant body weight on the ob/ob background, and at 8 weeks of age, they have an approximately 70% reduction in visceral and subcutaneous adipose tissues compared with ob/ob mice. Similarly, on the lethal yellow agouti (A(y)) background, FABP4-Wnt10b mice have 50-70% less adipose tissue weight and circulating leptin at 5 months of age. Wnt10b-Ay mice are more glucose tolerant and insulin sensitive than A(y) controls, perhaps due to reduced expression and circulation of resistin. Reduced expression of inflammatory cytokines may also contribute to improved glucose homeostasis.
Tumor-Derived Heat Shock Protein 70 Peptide Complexes Are Cross-Presented by Human Dendritic Cells (2002)

Our study demonstrates that tumor-derived heat shock protein (HSP)70 chaperones a tyrosinase peptide and mediates its transfer to human immature dendritic cells (DCs) by receptor-dependent uptake. Human tumor-derived HSP70 peptide complexes (HSP70-PC) thus have the immunogenic potential to instruct DCs to cross-present endogenously expressed, nonmutated, and tumor antigenic peptides that are shared among tumors of the melanocytic lineage for T cell recognition. T cell stimulation by HSP70-instructed DCs is dependent on the Ag bound to HSP70 in that only DCs incubated with HSP70-PC purified from tyrosinase-positive (HSP70-PC/tyr(+)) but not from tyrosinase-negative (HSP70-PC/tyr(-)) melanoma cells resulted in the specific activation of the HLA-A*0201-restricted tyrosinase peptide-specific cytotoxic T cell clone. HSP70-PC-mediated T cell stimulation is very efficient, delivering the tyrosinase peptide at concentrations as low as 30 ng/ml of HSP70-PC for T cell recognition. Receptor-dependent binding of HSP70-PC and active cell metabolism are prerequisites for MHC class I-restricted cross-presentation and T cell stimulation. T cell stimulation does not require external DC maturation signals (e.g., exogenously added TNF-alpha), suggesting that signaling DC maturation is an intrinsic property of the HSP70-PC itself and related to receptor-mediated binding. The cross-presentation of a shared human tumor Ag together with the exquisite efficacy are important new aspects for HSP70-based immunotherapy in clinical anti-cancer vaccination strategies, and suggest a potential extension of HSP70-based vaccination protocols from a patient-individual treatment modality to its use in an allogeneic setting.
Head Injury (1990)

Despite progress in the management of increased pressure following head injury, infection remains the most common complication in survivors. This study attempted to better define the immunologic deficit that occurs immediately and in the early post-recovery period following severe head injury. Twenty-seven patients admitted with the primary diagnosis of severe head injury (mean Glasgow Coma Score, 6.75) were studied within 24 hours of injury and at weekly intervals. T-cell proliferative response to mitogen stimulation was assessed. T-cell antigen expression following PHA stimulation was assessed for helper (CD4) and suppressor (CD8) subpopulations: the early T-cell activation antigens interleukin-2 receptor (IL2R) and transferrin receptor (TFR), and the late antigen (HLA-DR) using flow cytometry. Polymorphonuclear leukocyte function was assessed using oxidative burst. There was a reduction in the proliferative response of T cells to mitogen stimulation. The B-cell response seemed unaffected. The diminished proliferative response was accompanied by diminished expression of early activation antigens (IL2R and TFR) and late (following DNA synthesis) (HLA-DR). This was seen primarily on helper/inducer cells (CD4+). Polymorphonuclear leukocyte function was unaffected.
Upregulation of the RAS‐GTPase activating protein (GAP)‐binding protein (G3BP) in proliferating RPE cells (1999)

Cultured human retinal pigment epithelial (RPE) cells of different passages (P0 and P3) were used as a model system to examine changes in gene expression in proliferating RPE cells by polymerase chain reaction (PCR)-based differential expressed mRNA analysis (DEmRNA-PCR). DEmRNA-PCR showed enhanced expression of a specific RNA in P3 compared with P0. Sequence alignment displayed its identity with the 3′-end of the coding sequence of the human RAS-GTPase activating protein (GAP)-binding protein (G3BP). Confirmation of the induced expression of G3BP was performed by gene-specific reverse transcription-polymerase chain reaction (RT-PCR) of freshly prepared human RPE cells and of cultured cells of P0, P3 and P8 and by immunohistochemistry of cultivated retinal pigment epithelial cells in an artificial lesion assay. The expression of G3BP mRNA increased with the number of passages. G3BP protein expression increased in cells repopulating the artificial lesion. DEmRNA-PCR in RPE cells with subsequent sequence analysis led to the characterization of dedifferentiation- and proliferation-dependent expression of a previously undetected gene product in cultured RPE cells with a possible role in modifying signal transduction responses that may have implications on the treatment of proliferative vitreoretinopathy. J. Cell. Biochem. 74:194–201, 1999. © 1999 Wiley-Liss, Inc.
Coenzyme Q10 for Prevention of Anthracycline-Induced Cardiotoxicity (2005)

Preclinical and clinical studies suggest that anthracycline-induced cardiotoxicity can be prevented by administering coenzyme Q10 during cancer chemotherapy that includes drugs such as doxorubicin and daunorubicin. Studies further suggest that coenzyme Q10 does not interfere with the antineoplastic action of anthracyclines and might even enhance their anticancer effects. Preventing cardiotoxicity might allow for escalation of the anthracycline dose, which would further enhance the anticancer effects. Based on clinical investigation, although limited, a cumulative dose of doxorubicin of up to 900 mg/m2, and possibly higher, can be administered safely during chemotherapy as long as coenzyme Q10 is administered concurrently. The etiology of the dose-limiting cardiomyopathy that is induced by anthracyclines can be explained by irreversible damage to heart cell mitochondria, which differ from mitochondria of other cells in that they possess a unique enzyme on the inner mitochondrial membrane. This enzyme reduces anthracyclines to their semiquinones, resulting in severe oxidative stress, disruption of mitochondrial energetics, and irreversible damage to mitochondrial DNA. Damage to mitochondrial DNA blocks the regenerative capability of the organelle and ultimately leads to apoptosis or necrosis of myocytes. Coenzyme Q10, an essential component of the electron transport system and a potent intracellular antioxidant, appears to prevent damage to the mitochondria of the heart, thus preventing the development of anthracycline-induced cardiomyopathy.
Differentiation of myoblasts with nerve cells on microcarriers in culture. (1985)

Differentiation of embryonic rat and chick myoblasts was investigated using a tridimensional support made of positively charged, uncoated DEAE-cellulose microcarriers (MC). Following rapid cell attachment, the MC interconnected to form large cell-MC conglomerates which remained floating in the nutrient medium. Cells within the conglomerates fused to form myotubes which synthesized muscle-specific proteins such as: creatine kinase, acetylcholinesterase, acetylcholine receptors, and contracted in response to electrical stimulation. Myotubes, at different stages of differentiation, showed characteristic morphology (as observed by transmission and scanning electron-microscopies). Upon addition of dissociated spinal cord cells to these muscle-MC cultures, intensive sprouting of nerve fibres took place. After a few days an extensive network of nerve fibres was formed on the top of muscle myotubes and nerve-muscle contacts were established.
Parallel Synthesis of Alkyl Tetrazole Derivatives Using Solid Support Chemistry (1999)

The synthesis of several omega-chloroalkyl tetrazoles and their subsequent attachment to a solid support is described. Using an in situ Finkelstein reaction, a variety of nucleophiles were alkylated and then cleaved from the resin to give pure alkyl tetrazole derivatives. A sample library of 5 x 6 demonstrates the general utility of this sequence.
Variable antigen genes of the relapsing fever agent Borrelia hermsii are activated by promoter addition (1991)

Borrelia hermsii, an agent of relapsing fever, avoids the host's immune response by means of multiphasic antigenic variation. Serotype specificity is determined by variable antigens called the Vmp lipoproteins. Through recombination between linear plasmids a formerly silent vmp gene replaces another vmp gene at a telomeric expression locus. We examined strain HS1 borreliae before and after a switch from serotype 7 to serotype 21. The nucleotide sequences of 5' regions of silent and expressed vmp7 and vmp21 were determined. Silent and active vmp7 and vmp21 genes shared a block of homologous sequences surrounding their 5' ends. Sequences upstream of silent vmp7 and vmp21 genes lacked the promoter and substantially differed from each other. In this antigenic switch a vmp gene was activated by a recombination that placed it downstream of a promoter.
Mutational analysis of the role of ADP-ribosylation activity and GM1 -binding activity in the adjuvant properties of theEscherichia coli heat-labile enterotoxin towards intranasally administered keyhole limpet hemocyanin (1998)

The Escherichia coli heat-labile enterotoxin (LT) is known for its potent mucosal immunoadjuvant activity towards co-administered antigens. LT is composed of one A subunit, which has ADP-ribosylation activity, and a homopentameric B subunit, which has high affinity for the toxin receptor, ganglioside GM1. In previous studies, we have investigated the role of the LTA and LTB subunits in the adjuvanticity of LT towards influenza virus hemagglutinin (HA), administered intranasally to mice. We now studied the adjuvant properties of LT and LT variants towards keyhole limpet hemocyanin (KLH), which, in contrast to HA, does not bind specifically to mucosal surfaces. It is demonstrated that LT mutants without ADP-ribosylation activity, as well as LTB, retain mucosal immunoadjuvant activity when administered intranasally to mice in conjunction with KLH. As with influenza HA, adjuvanticity of LTB required GM1-binding activity, whereas GM1-binding was not essential for adjuvant activity of LT. Furthermore, we found that also recombinant LTA alone acts as a potent mucosal adjuvant, and that this adjuvanticity is independent of ADP-ribosylation activity. It is concluded that binding of the antigen to mucosal surfaces does not play an essential role in the immunostimulation by LT and LT variants, and that both recombinant LTA and LTB represent powerful nontoxic mucosal adjuvants.
Preferential expression of a plant cystatin at nematode feeding sites confers resistance to Meloidogyne incognita and Globodera pallida (2003)

The expression patterns of three promoters preferentially active in the roots of Arabidopsis thaliana have been investigated in transgenic potato plants in response to plant parasitic nematode infection. Promoter regions from the three genes, TUB-1, ARSK1 and RPL16A were linked to the GUS reporter gene and histochemical staining was used to localize expression in potato roots in response to infection with both the potato cyst nematode, Globodera pallida and the root-knot nematode, Meloidogyne incognita. All three promoters directed GUS expression chiefly in root tissue and were strongly up-regulated in the galls induced by feeding M. incognita. Less activity was associated with the syncytial feeding cells of the cyst nematode, although the ARSK1 promoter was highly active in the syncytia of G. pallida infecting soil grown plants. Transgenic potato lines that expressed the cystatin OcIDeltaD86 under the control of the three promoters were evaluated for resistance against Globodera sp. in a field trial and against M. incognita in containment. Resistance to Globodera of 70 +/- 4% was achieved with the best line using the ARSK1 promoter with no associated yield penalty. The highest level of partial resistance achieved against M. incognita was 67 +/- 9% using the TUB-1 promoter. In both cases this was comparable to the level of resistance achieved using the constitutive cauliflower mosaic virus 35S (CaMV35S) promoter. The results establish the potential for limiting transgene expression in crop plants whilst maintaining efficacy of the nematode defence.
Experiments on Learning by Back Propagation. (1986)

Rumelhart, Hinton and Williams [Rumelhart et al. 86] describe a learning procedure for layered networks of deterministic, neuron-like units. This paper describes further research on the learning procedure. We start by describing the units, the way they are connected, the learning procedure, and the extension to iterative nets. We then give an example in which a network learns a set of filters that enable it to discriminate formant-like patterns in the presence of noise. The speed of learning is strongly dependent on the shape of the surface formed by the error measure in &amp;amp;quot;weight space. &amp;amp;quot; We give examples of the shape of the error surface for a typical task and illustrate how an acceleration method speeds up descent in weight space. The main drawback of the learning procedure is the way it scales as the size of the task and the network increases. We give some preliminary results on scaling and show how the magnitude of the optimal weight changes depends on the fan-in of the units. Additional results illustrate the effects on learning speed of the amount of interaction between the weights. A variation of the learning procedure that back-propagates desired state information rather than error gradients is developed and compared with the standard procedure. Finally, we discuss the relationship between our iterative networks and the &amp;amp;quot;analog &amp;amp;quot; networks described by Hopfield and Tank [Hopfield and Tank 85]. The learning procedure can discover appropriate weights in their kind of network, as well as determine an optimal schedule for varying the nonlinearity of the units during a search. 1.