gebhart/patrepeval huggingface dataset:
A simulation model for predicting the effect of advertisement schedules (1977)

Advertisement Scheduling refers to the detailing of where advertisements for a product/idea should appear in different media vehicles (TV shows, magazines, etc.) so as to maximize the effect on the target population. The effect is usually hoped to be favorable and influence the population to go out and buy the product/idea.Media scheduling models provide solutions that describe the number of advertisements to be inserted in different media vehicles over a given time horizon. These models do not specify the precise timing of the insertions, as it is usually assumed that within a given time interval there are not many choices for any one media option. (e.g. there is only one Reader's Digest each month).But if TV or radio is considered the situation becomes more difficult. The number of choices even within a week is large (e.g. there are five “To-night” shows in a week); and many of the desired spots may not be available because of high demand. Therefore the detailed schedules which take into consideration the availability of specific spots must be evaluated. As the relationships that exist between exposure to advertisements, their timing and the effect on the individuals are complex analytical methods are not very useful.The simulation model described below evaluates different advertisement schedules for their effect on the target population. The effect can be in terms of any one of a number of variables - probability of purchase, awareness of brand's advertisement, intention to purchase etc. In this model awareness of the brand's advertisement is used as the desired variable. Although not directly influencing the market share of the brand, this variable is considered quite important by marketing managers.The model is a micro-simulation model - it stimulates the responses of individuals from the sample population. It can simulate single period and multi-periods.
Nucleotide sequence of a secondary attachment site for bacteriophage lambda on the Escherichia coli chromosome (1979)

The nucleotide sequence of a secondary attachment site for bacteriophage lambda was determined in a region near the rrnB gene at 88 min on the E. coli chromosome. The sequence has a 8 base pair interrupted homology GCT TTTTA to the common core of the primary attachment site (attB) and the corresponding phage sequence (attP). The site of crossover during integration lies probably between nucleotides −3 and +1. The flanking regions have no obvious homology to the arms of either attP or attB.
Acyl-coenzyme A:cholesterol acyltransferases (2009)

The enzymes acyl-coenzyme A (CoA):cholesterol acyltransferases (ACATs) are membrane-bound proteins that utilize long-chain fatty acyl-CoA and cholesterol as substrates to form cholesteryl esters. In mammals, two isoenzymes, ACAT1 and ACAT2, encoded by two different genes, exist. ACATs play important roles in cellular cholesterol homeostasis in various tissues. This chapter summarizes the current knowledge on ACAT-related research in two areas: 1) ACAT genes and proteins and 2) ACAT enzymes as drug targets for atherosclerosis and for Alzheimer's disease.
Identification of a Gene Cluster for the Biosynthesis of a Long, Galactose-Rich Exopolysaccharide in Lactobacillus rhamnosus GG and Functional Analysis of the Priming Glycosyltransferase (2009)

Cell surface polysaccharides have an established role as virulence factors in human bacterial pathogens. Less documented are the biosynthesis and biological functions of surface polysaccharides in beneficial bacteria. We identified a gene cluster that encodes the enzymes and regulatory and transporter proteins for the different steps in the biosynthesis of extracellular polysaccharides (EPS) of the well-documented probiotic strain Lactobacillus rhamnosus GG. Subsequent mutation of the welE gene, encoding the priming glycosyltransferase within this cluster, and comparative phenotypic analyses of wild-type versus mutant strains confirmed the specific function of this gene cluster in the biosynthesis of high-molecular-weight, galactose-rich heteropolymeric EPS molecules. The phenotypic analyses included monomer composition determination, estimation of the polymer length of the isolated EPS molecules, and single-molecule force spectroscopy of the surface polysaccharides. Further characterization of the welE mutant also showed that deprivation of these long, galactose-rich EPS molecules results in an increased adherence and biofilm formation capacity of L. rhamnosus GG, possibly because of less shielding of adhesins such as fimbria-like structures.
Tumor antigen presentation by murine epidermal cells (1991)

The ability of epidermal Langerhans cells to present Ag for CD4-dependent immunity is well documented, and it has been hypothesized that Langerhans cells participate in the generation of immunity against incipient epidermal neoplasms by presentation of tumor-associated Ag in situ. This study examined the ability of murine epidermal cells (EC) to present tumor-associated Ag for the induction of in vivo antitumor immunity. Murine epidermal cells were deleted of Thy-1-bearing cells, cultured in 50 U/ml granulocyte-macrophage-CSF for 14 to 18 h, and pulsed with tumor fragments (TF) derived from S1509a-fibrosarcoma cells. These TF-pulsed EC were injected s.c. into syngeneic recipients at weekly intervals for a total of three immunizations and challenged with viable S1509a tumor cells 1 wk after the last immunization. Control animals received TF-pulsed allogeneic EC or EC treated identically but not pulsed with TF. EC that were pulsed with tumor cell fragments were able to induce protective immunity to tumor growth in vivo and to immunize for a significant delayed-type hypersensitivity response to injected tumor cells. The induction of antitumor immunity with TF-pulsed EC was genetically restricted, and culture of EC in granulocyte-macrophage-CSF was required for development of significant immunity. Furthermore, deletion of I-A+ cells by antibody and complement-mediated lysis eliminated the generation of immunity. Thus, I-A+ epidermal cells are capable of presenting S1509a tumor Ag for the generation of protective antitumor immunity in vivo.
MONITORING OF SOLUBLE HLA ALLOANTIGENS AND ANTI-HLA ANTIBODIES IDENTIFIES HEART ALLOGRAFT RECIPIENTS AT RISK OF TRANSPLANT-ASSOCIATED CORONARY ARTERY DISEASE1 (1996)

The development of accelerated transplant-related coronary artery disease (T-CAD) is the major obstacle to long-term survival of cardiac allografts. We have investigated the role of various demographic and immunologic parameters as prognostic indicators of T-CAD in a population of 274 heart allograft recipients. Our data demonstrate that patients who experience more than 1 episode of acute rejection per year and/or develop antidonor HLA antibodies are at increased risk of developing T-CAD. Using HLA-A2 as a marker for the release of soluble HLA antigens from the donor, we established that recipients displaying circulating donor alloantigens for more than 26 weeks following transplantation are at increased risk of developing T-CAD (P=0.008). This association suggests that the release of alloantigens from the allograft is indicative of chronic injury and/or that it stimulates chronic rejection via the indirect allorecognition pathway. Our findings indicate that patients at risk of developing T-CAD can be identified by monitoring the release of donor alloantigens and production of antidonor HLA antibodies following transplantation.
CPT-11 in human colon-cancer cell lines and xenografts: Characterization of cellular sensitivity determinants (1997)

CPT-11, a new semisynthetic derivative of camptothecin, is active in a number of tumor types in the clinic, including colon cancer. CPT-11 is a drug that is converted into the active metabolite SN-38 by a carboxylesterase. Experiments were performed to obtain more insight in the cellular characteristics in 5 unselected human colon-cancer cell lines that account for the differential sensitivity to CPT-11 and SN-38. In vitro, the sensitivity to CPT-11 and SN-38 was highest in LS174T and COLO 320 cells, intermediate in SW1398 cells and lowest in COLO 205 and WiDr cells. SN-38 was 130 to 570 times more active than CPT-11. CPT-11 induced complete remissions in 6 out of 12 COLO 320 tumors grown as subcutaneous xenografts, but was not effective in WiDr tumors. The cellular carboxylesterase activity did not relate to the sensitivity to CPT-11. The enzyme activity was higher in normal mouse tissues, i.e., serum and liver, than in COLO 320 or WiDr xenografts, indicating that tumor carboxylesterase is of minor importance for CPT-11 efficacy. The topoisomerase-1 mRNA expression in tumor cells was not predictive of the antiproliferative effects of CPT-11 or SN-38. We observed a positive relationship between the DNA topoisomerase-1 activity and the cellular sensitivity to carboxylesterase-activated CPT-11 (r = 0.75, p < 0.1) as well as to SN-38 (r = 0.89, p < 0.05). The higher topoisomerase-1 activity in COLO 320 cells and tumors when compared with that in WiDr cells and tumors reflected the differences in sensitivity to the drug(s). In conclusion, the DNA topoisomerase-1 activity was the best determinant for CPT-11/SN-38 sensitivity in this panel of unselected human colon-cancer cell lines.
Studies on Heterotypic Interference between Influenza A and B Viruses: A Differential Inhibition of the Synthesis of Viral Proteins and RNAs (1983)

Mixed infection of MDCK cells with influenza A and influenza B viruses leads to a reduction in the rate of synthesis of haemagglutinin (HA) and nucleoprotein (NP) as compared to their rate of synthesis in cells separately infected with these viruses. The reduction is much stronger for influenza A virus proteins. The synthesis of the nonstructural NS1 protein of both viruses is relatively resistant to the heterotypic interference. The synthesis of virus-specific mRNAs exhibits the same pattern: the formation of the transcripts of HA and NP genes is much more drastically reduced than the synthesis of NS gene transcripts. The effect is strongly dependent on the multiplicity of infection and on the ratio of influenza A and B viruses in the inoculum. Primary transcription in the presence of cycloheximide is almost unchanged in doubly infected cells as compared to single infection, and no indication of differential inhibition has been observed. The results are discussed in connection with the mechanism of heterotypic interference and the regulation of influenza virus protein synthesis.
Use of Oligonucleotides Carrying Photolabile Groups for the Control of the Deposition of Nanoparticles in Surfaces and Nanoparticle Association (2011)

An oligodeoxynucleotide hairpin containing a photolabile 2-nitrobenzyl group in the loop and terminated with a thiol function was prepared. The photocleavage of such a hairpin on gold yields a surface activated with a single stranded oligonucleotide which can be utilised to direct the assembly of nanoparticles conjugated with a complementary strand. Analysis of photocleaved surfaces gives nanoparticle coverage one order of magnitude higher than nonphotocleaved surfaces. This illustrates the ability of photocleavable hairpins to direct the assembly of nanomaterials on conducting materials. The conjugation of the photocleavable hairpin to a gold nanoparticle allows the observation of intermolecular interactions between hairpins linked in different nanoparticles, by comparing the thermal dissociations of a hairpin-nanoparticle conjugates at 260 nm and 520 nm. We have also shown that it is possible to permanently alter the physiochemical properties of DNA-nanoparticles by the introduction of a photocleavable group. Indeed for the first time it has been shown that by exposure to UV light the disassembly of nanoparticle aggregates can be induced.
Raman Spectroscopy for the in Situ Identification of Cocaine and Selected Adulterants (2000)

We demonstrate the in situ identification of crack cocaine and cocaine·HCl by using a fiber-optic Raman probe and a portable Raman spectrograph. The Raman spectrum of freebase cocaine (crack) is obtained in just seconds without any sample preparation, and differs significantly from that of cocaine·HCl. We also show that the Raman spectra of these drugs are easily distinguishable from common cutting agents and impurities such as benzocaine and lidocaine. Another advantage of using Raman spectroscopy is that the drugs can be identified while contained in transparent containers, such as clear plastic evidence containers that are used to store drug evidence and to maintain chain of custody. We also demonstrate the in situ Raman identification of drugs separated by thin-layer chromatography. We discuss the utility of surface-enhanced Raman spectroscopy (SERS) in toxicological drug screening and present preliminary SERS data for cocaine in solution using colloidal silver. We believe this to be the first published SERS spectrum of freebase cocaine.
Improved Body Distribution of14C-labelled AZT bound to Nanoparticles in Rats determined by Radioluminography (1998)

The objective of the present study is to visualize differences in the body distribution between radiolabelled AZT bound to nanoparticles and a control solution. Polyhexylcyanoacrylate nanoparticles were manufactured by emulsion polymerization in the presence of AZT and an ionic emulsifier, bis(2-ethylhexyl) sulfosuccinate sodium. The AZT-control solution was equally prepared, but contained no monomer. The two preparations were administered either by i.v. injection or perorally by gavage. After determined time points the animals were sacrificed using carbon dioxide. The cadavers were shock-frozen in cellulose gel and cut into slices using a cryomicrotome. The tissue cross sections were fixed on an adhesive tape and then were freeze dried. The quantification of the radioactive AZT in the different organs and tissues was performed by radioluminography, and the images were generated on a computer. After i.v. injection of AZT-nanoparticles, a high amount of the AZT label was found in the organs belonging to the reticuloendothelial system. In these organs the radioactivity was inhomogeneously distributed showing that the uptake of the particle-associated radioactivity depended on the type of the cells located in the organs and was consistent with uptake by macrophages. The highest radioactivities were found in the GI-tract and in the liver. A difference in the elimination pathway between AZT-control solution and AZT bound to nanoparticles also was visible on the images. Similar results were obtained after oral administration. Of course, with the latter route a larger portion of AZT remained in the GI-tract especially after administration of nanoparticle-bound drug. These results confirmed those obtained by a classically performed quantitative whole body distribution study using liquid scintillation. This demonstrates that radioluminography is a useful method to study the organ distribution of drugs bound to nanoparticles.
Long-acting testosterone undecanoate for parenteral testosterone therapy (2006)

Testosterone as a compound for the treatment of testosterone deficiency has been available for almost 70 years; however, the pharmaceutical formulations have been less than ideal. Injectable testosterone esters have been used traditionally for treatment, but they generate supranormal testosterone levels shortly after the 2–3-weekly injections, when testosterone levels then decline very rapidly becoming subnormal in the days before the next injection. Testosterone undecanoate is a new injectable testosterone preparation with a considerably better pharmacokinetic profile. After two initial injections with a 6-week interval, the following intervals between two injections are almost always 12 weeks. Plasma testosterone levels with this preparation are almost always in the normal range. Side effects experienced with conventional testosterone esters are almost nil with this preparation.
Purification, crystallization and preliminary X-ray analysis of the Fab fragment from MNAC13, a novel antagonistic anti-tyrosine kinase A receptor monoclonal antibody (2001)

The monoclonal antibody MNAC13 is a potent antagonist that prevents the binding of nerve-growth factor (NGF) to its tyrosine kinase A receptor (TrkA) in a variety of systems. Structural studies of the FabMNAC13 fragment were performed to gain insights into the mechanism of action of this potentially therapeutic monoclonal antibody. The optimal conditions for crystallization of FabMNAC13 were determined. Crystals appeared as prismatic bundles, displayed P2(1)2(1)2(1) space-group symmetry and diffracted to a resolution of 1.8 A. The unit-cell parameters were determined to be a = 52.73, b = 67.55, c = 111.43 A. The data set was 99.5% complete. Molecular replacement was performed, resulting in a correlation coefficient of 0.55 and an R value of 0.40. The structure refinement is now in progress.
ISG15 enhances the innate antiviral response by inhibition of IRF-3 degradation. (2006)

The transcription factor, interferon regulatory Factor 3 (IRF-3) plays a critical role in the activation of an antiviral innate immune response. However the transcriptional activity of IRF-3 is tightly regulated by a proteosome mediated degradation. We describe here a novel mechanism by which the activity of IRF-3 is stabilized in infected cells. We have shown that both interferon treatment and NDV infection profoundly increase conjugation of interferon induced ubiquitin- like protein ISG15 to cellular proteins. ISGylated IRF-3 could be detected both in interferon treated and virus-infected cells. ISG15, subverts the ubiquitin mediated degradation of IRF-3 in NDV infected 2fTGH cells and enhances the NDV mediated transactivation of interferonbeta promoter and the translocation of activated IRF-3 to the nucleus. The relative levels of IRF-3 were significantly lower in NDV infected ISG15 null MEF, than in wt MEF. While ISG15 null MEF were more permissive to VSV replication their sensitivity to the antiviral effect of interferon was not modulated. These results reveal that virus mediated subversion of the antiviral response by proteolysis of IRF-3 is counteracted by induction of ISG15 expression and that ISGylation provides a feedback mechanism, which enhances the host innate antiviral response via IRF-3 stabilization.
Manipulation of Human Ovarian Function: Physiological Concepts and Clinical Consequences* (1997)

I. Introduction II. Dynamics of Normal Human Follicle Growth and Selection A. Gonadotropin-independent and -dependent follicle growth B. Intrafollicular endocrine changes C. Are estrogens needed for follicle development? D. In vivo regulation of follicle maturation in the monkey E. The FSH threshold and window concept for in vivo follicle growth 1. FSH threshold and follicle recruitment 2. FSH window and single dominant follicle selection 3. Dominant follicle development F. Modulation of FSH action 1. Heterogeneity of FSH 2. Direct interference with FSH action 3. Intraovarian interference with FSH action by growth factors III. Gonadotropin Induction of Ovulation A. The concept of monofollicle growth in anovulatory patients B. Conditions affecting treatment outcome 1. Patient-related factors 2. Hormone preparation-related factors 3. Other factors involved C. Commonly used step-up dose regimens 1. Conventional step-up protocol 2. Low-dose, step-up protocol D. Potential for a step-down dose regimen IV. Stero...
Pyrimidine Salvage Pathways In Toxoplasma Gondii (1993)

Pyrimidine salvage enzyme activities in cell-free extracts of Toxoplasma gondii were assayed in order to determine which of these enzyme activities are present in these parasites. Enzyme activities that were detected included phosphoribosyltransferase activity towards uracil (but not cytosine or thymine), nucleoside phosphorylase activity towards uridine, deoxyuridine and thymidine (but not cytidine or deoxycytidine), deaminase activity towards cytidine and deoxycytidine (but not cytosine, cytidine 5'-monophosphate or deoxycytidine 5'-monophosphate), and nucleoside 5'-monophosphate phosphohydrolase activity towards all nucleotides tested. No nucleoside kinase or phosphotransferase activity was detected, indicating that T. gondii lack the ability to directly phosphorylate nucleosides. Toxoplasma gondii appear to have a single non-specific uridine phosphorylase enzyme which can catalyze the reversible phosphorolysis of uridine, deoxyuridine and thymidine, and a single cytidine deaminase activity which can deaminate both cytidine and deoxycytidine. These results indicate that pyrimidine salvage in T. gondii probably occurs via the following reactions: cytidine and deoxycytidine are deaminated by cytidine deaminase to uridine and deoxyuridine, respectively; uridine and deoxyuridine are cleaved to uracil by uridine phosphorylase; and uracil is metabolized to uridine 5'-monophosphate by uracil phosphoribosyltransferase. Thus, uridine 5'-monophosphate is the end-product of both de novo pyrimidine biosynthesis and pyrimidine salvage in T. gondii.
Depth Image Based Rendering for 3D Data Service Over T-DMB (2008)

In this paper, we present a depth image based rendering technique for 3D data service over T-DMB. 3D service over T-DMB is very attractive because the single user environment of T-DMB is suitable to glassless 3D viewing. However, the bit budget for depth transmission over T-DMB is very limited with 32 Kbps for data service. Depth image based rendering can resolve this problem because the corresponding depth sequence can be compressed effectively. However, depth image based rendering has also some problems such as a large computational cost for generating virtual images and generation of holes by disocclusion. Therefore, we propose the new depth preprocessing method based on adaptive smoothing and the simultaneous method for generating an auto-stereoscopic image. In the proposed depth preprocessing method, the discontinuity preserving smoothing, followed by the adaptive smoothing based on gradient direction, is conducted by generalizing the conduction function of the anisotropic smoothing. As a result, the reduction of both bit rate required for transmission and holes is achieved. Additionally, the simultaneous method for generating an auto-stereoscopic image can resolve problems of limited memory and large computational complexity. Some experiments show that the proposed scheme can be efficiently employed for 3D data service over T-DMB.