gebhart/patrepeval huggingface dataset:
Cytotoxicity of rhenium(I) alkoxo and hydroxo carbonyl complexes in murine and human tumor cells. (2000)

The rhenium(I) alkoxo/hydroxo carbonyl complexes were shown to be very potent in suspended tumor cell lines in suppressing growth but were more selective in inhibiting the growth of cultures from solid tumors. Their mode of action in L1210 lymphoid leukemia cells indicated that they were not alkylating agents but interfered with nucleic acid metabolism at multiple enzyme sites, e.g. dihydrofolate reductase, PRPP-amido transferase, thymidine kinase, with DNA strand scission after 60 min incubation. These compounds did not function mechanistically exclusively as cisplatin derivatives causing intrastrand linkages of DNA but rather they mimicked the metal complexes of aminecarboxyboranes, furan oximes, N-substituted thiosemicarbazones, trifluoromethyl borons and ferratricarbadecarbanyl complexes acting as antimetabolites.
Value of the Modified Hodge Test for Detection of Emerging Carbapenemases in Enterobacteriaceae (2011)

The modified Hodge test has an excellent sensitivity for detecting enterobacterial isolates producing Ambler class A (KPC) and class D (OXA-48) carbapenemases. Its sensitivity is low for NDM-1 producers (50%) but is increased to 85.7% by adding ZnSO(4) (100 μg/ml) in the culture medium. However, this test has a low specificity and is time-consuming.
Intermixing at the tantalum oxide/silicon interface in gate dielectric structures (1998)

Metal oxides with high dielectric constants have the potential to extend scaling of transistor gate capacitance beyond that of ultrathin silicon dioxide. However, during deposition of most metal oxides on silicon, an interfacial region of SiOx can form that limits the specific capacitance of the gate structure. We have examined the composition of this layer using high-resolution depth profiling of medium ion energy scattering combined with infrared spectroscopy and transmission electron microscopy. We find that the interfacial region is not pure SiO2, but is a complex depth-dependent ternary oxide of Si–Tax–Oy with a dielectric constant at least twice that of pure SiO2 as inferred from electrical measurements. High-temperature annealing crystallizes the Ta2O5 film and converts the composite oxide to a more pure SiO2 layer with a lower capacitance density. Using low postanneal temperatures, a stable composite oxide structure can be obtained with good electrical properties and an effective SiO2 thickness of less than 2 nm with ∼10 nm of composite oxide.
C-Type Natriuretic Peptide Plasma Levels Increase in Patients with Chronic Heart Failure as a Function of Clinical Severity (2005)

Plasma CNP elevation is related to clinical and functional disease severity. These findings suggest a pathophysiological role for this peptide that, for its vasorelaxing activity, could influence the endothelial vasomotor response in CHF.
Prodrug Strategies for Enhancing the Percutaneous Absorption of Drugs (2014)

The transdermal application of drugs has attracted increasing interest over the last decade or so, due to the advantages it offers, compared to other delivery methods. The development of an efficient means of transdermal delivery can increase drug concentrations, while reducing their systemic distribution, thereby avoiding certain limitations of oral administration. The efficient barrier function of the skin, however, limits the use of most drugs as transdermal agents. This limitation has led to the development of various strategies to enhance drug-skin permeation, including the use of penetration enhancers. This method unfortunately has certain proven disadvantages, such as the increased absorption of unwanted components, besides the drug, which may induce skin damage and irritancy. The prodrug approach to increase the skin's permeability to drugs represents a very promising alternative to penetration enhancers. The concept involves the chemical modification of a drug into a bioreversible entity that changes both its pharmaceutical and pharmacokinetic characteristics to enhance its delivery through the skin. In this review; we report on the in vitro attempts and successes over the last decade by using the prodrug strategy for the percutaneous delivery of pharmacological molecules.
Software radio architecture with smart antennas: a tutorial on algorithms and complexity (1999)

There has been considerable interest in using antenna arrays in wireless communication networks to increase the capacity and decrease the cochannel interference. Adaptive beamforming with smart antennas at the receiver increases the carrier-to-interference ratio (CIR) in a wireless link. This paper considers a wireless network with beamforming capabilities at the receiver which allows two or more transmitters to share the same channel to communicate with the base station. The concrete computational complexity and algorithm structure of a base station are considered in terms of a software radio system model, initially with an omnidirectional antenna. The software radio computational model is then expanded to characterize a network with smart antennas. The application of the software radio smart antenna is demonstrated through two examples. First, traffic improvement in a network with a smart antenna is considered, and the implementation of a hand-off algorithm in the software radio is presented. The blocking probabilities of the calls and total carried traffic in the system under different traffic policies are derived. The analytical and numerical results show that adaptive beamforming at the receiver reduces the probability of blocking and forced termination of the calls and increases the total carried traffic in the system. Then, a joint beamforming and power control algorithm is implemented in a software radio smart antenna in a CDMA network. This shows that, by using smart antennas, each user can transmit with much lower power, and therefore the system capacity increases significantly.
Intracellular cyclic AMP concentration is decreased in Salmonella typhimurium fur mutants The GenBank accession number for the sequence reported in this paper is AF268282. (2002)

It is known that the Fur protein negatively regulates iron-uptake systems in different bacterial species, including Salmonella typhimurium. In this study it has been shown that the intracellular concentration of cyclic AMP (cAMP) is lower in a knockout S. typhimurium fur mutant than in the wild-type strain. According to this, the expression of two cAMP-regulated genes, such as pepE (encoding an alpha-aspartyl dipeptidase) and the Escherichia coli lac operon, is decreased in S. typhimurium fur cells in comparison with wild-type cells. Introduction of an additional mutation in cpdA, encoding a cyclic 3',5'-cAMP phosphodiesterase, recovers wild-type intracellular cAMP concentration in the S. typhimurium fur mutant. Likewise, expression of pepE and the E. coli lac operon was the same in the S. typhimurium fur cpdA double mutant and the wild-type strain. Moreover, these results also demonstrate that the S. typhimurium Fur protein positively regulates the expression of the flhD master operon governing the flagellar regulon. This positive control must be mediated by binding of the S. typhimurium Fur protein to the flhD promoter as indicated by the fact that this promoter tests positive in a Fur titration assay.
Improved lumen visualization in metallic vascular implants by reducing RF artifacts (2001)

In this study, a method is proposed for MRI of the lumen of metallic vascular implants, like stents or vena cava filters. The method is based on the reduction of artifacts caused by flow, susceptibility, and RF eddy currents. Whereas both flow artifacts and susceptibility artifacts are well understood and documented, RF artifacts are not. Therefore, the present study comprises an in-depth theoretical explanation of the factors governing the severity of these RF artifacts. It is explained that the RF caging inside cage-like implants is caused by disturbances of the send and receive sensitivities due to coupling between the loops in the implant and the MR scanner's send and receive coils. A scaled excitation angle model describing the behavior of the signal intensity inside the implants as a function of the applied nominal excitation angle is introduced. This theoretical model was validated in phantom experiments. Reduced signal from within implants due to the caging problem could be restored by increasing the applied RF power in the excitation pulse, without exceeding the generally accepted SAR safety limits. The method was tested in vitro and in vivo in a pig model and allowed adequate depiction of the interior of a nitinol stent and that of a vena cava filter in contrast-enhanced MR angiograms. Magn Reson Med 47:171-180, 2002.
Twenty-five milligrams of clomiphene citrate presents positive effect on treatment of male testosterone deficiency - a prospective study (2012)

The CC was effective in stimulating the endogenous production of testosterone. A lower level of total cholesterol was verified after three months of treatment. This medication should be considered as a therapeutic option for some patients with symptomatic male testosterone deficiency.
Modification of pre-mRNA splicing by antisense oligonucleotides. (1997)

Antisene oligonucleotides have been extensively studied as agents that inhibit the expression of undesirable genes in a sequence specific manner. Results reviewed in this article show that antisene oligonucleotides can also restore the expression of genes inactivated by mutations causing genetic diseases. In this novel application, antisene oligonucleotides block aberrant splice sites created by the mutations, forcing the spliceosomes to form at correct splice sites, thus restoring the proper splicing pathway and consequently the activity of the damaged gene.
EpCAM and α-Fetoprotein Expression Defines Novel Prognostic Subtypes of Hepatocellular Carcinoma (2008)

The heterogeneous nature of hepatocellular carcinoma (HCC) and the lack of appropriate biomarkers have hampered patient prognosis and treatment stratification. Recently, we have identified that a hepatic stem cell marker, epithelial cell adhesion molecule (EpCAM), may serve as an early biomarker of HCC because its expression is highly elevated in premalignant hepatic tissues and in a subset of HCC. In this study, we aimed to identify novel HCC subtypes that resemble certain stages of liver lineages by searching for EpCAM-coexpressed genes. A unique signature of EpCAM-positive HCCs was identified by cDNA microarray analysis of 40 HCC cases and validated by oligonucleotide microarray analysis of 238 independent HCC cases, which was further confirmed by immunohistochemical analysis of an additional 101 HCC cases. EpCAM-positive HCC displayed a distinct molecular signature with features of hepatic progenitor cells including the presence of known stem/progenitor markers such as cytokeratin 19, c-Kit, EpCAM, and activated Wnt-beta-catenin signaling, whereas EpCAM-negative HCC displayed genes with features of mature hepatocytes. Moreover, EpCAM-positive and EpCAM-negative HCC could be further subclassified into four groups with prognostic implication by determining the level of alpha-fetoprotein (AFP). These four subtypes displayed distinct gene expression patterns with features resembling certain stages of hepatic lineages. Taken together, we proposed an easy classification system defined by EpCAM and AFP to reveal HCC subtypes similar to hepatic cell maturation lineages, which may enable prognostic stratification and assessment of HCC patients with adjuvant therapy and provide new insights into the potential cellular origin of HCC and its activated molecular pathways.
Exploring the Specificity Pockets of Two Homologous SH3 Domains Using Structure-Based, Split-Pool Synthesis and Affinity-Based Selection (1998)

Split-pool synthesis was used to prepare large numbers of spatially-separated molecules and thereby to investigate the specificity pockets of similar SH3 domains found in the tyrosine kinases Src and Hck. By taking into account the structure of the Src SH3 domain complexed to a ligand containing non-peptide-binding elements, the molecules were designed to complement the topography of the protein's binding pocket. This procedure led to the discovery of ligands having greater affinity and enhanced selectivity for the Src SH3 domain. It also yielded non-natural ligands that bind selectively to the Hck SH3 domain. Insights gained from this strategy may facilitate the discovery of molecules useful for evaluating the cellular function of SH3 domain-containing proteins.
Making a Back-Illuminated Imager with Back-Side Contact and Alignment Markers (2008)

A design modification and a fabrication process that implements the modification have been conceived to solve two problems encountered in the development of back-illuminated, back-sidethinned complementary metal oxide/ semiconductor (CMOS) image-detector integrated circuits. The two problems are (1) how to form metal electrical-contact pads on the back side that are electrically connected through the thickness in proper alignment with electrical contact points on the front side and (2) how to provide alignment keys on the back side to ensure proper registration of backside optical components (e.g., microlenses and/or color filters) with the front-side pixel pattern. The essence of the design modification is to add metal plugs that extend from the desired front-side locations through the thickness and protrude from the back side of the substrate. The plugs afford the required front-to-back electrical conduction, and the protrusions of the plugs serve as both the alignment keys and the bases upon which the back-side electrical-contact pads can be formed.
Troglitazone inhibits angiotensin II‐induced DNA synthesis and migration in vascular smooth muscle cells (1997)

Angiotensin II (AII) plays a crucial role in controlling the proliferation and migration of vascular smooth muscle cells (VSMCs). The present study was undertaken to determine if troglitazone (Tro) has an effect on the G-protein coupled signaling through AII type I (AT-1) receptors in cultured rat aortic VSMCs. AII-induced MAP kinase activation was inhibited 67.9% by Tro. AII-induced DNA synthesis and migration was completely inhibited by Tro or by the AT-1 receptor blocker irbesartan. The present study demonstrates that troglitazone inhibits AII-induced DNA synthesis, migration and MAP kinase activation in VSMCs which are important molecular events for the development of neointimal hyperplasia and atherosclerosis.
The Serine Protease HtrA1 Specifically Interacts and Degrades the Tuberous Sclerosis Complex 2 Protein (2010)

Hamartin and tuberin are products of the tumor suppressor genes TSC1 and TSC2, respectively. Mutations affecting either gene result in the tuberous sclerosis syndrome, a neurologic genetic disorder characterized by the formation of multiple benign tumors or hamartomas. In this study, we report the identification of TSC2, but not TSC1, as a substrate of HtrA1, a member of the human HtrA family proteins of serine proteases. We show the direct interaction and colocalization in the cytoplasm of HtrA1 and TSC2 and that HtrA1 cleaves TSC2 both in vitro and in vivo. Finally, we show that alterations in HtrA1 expression cause modifications in phosphorylation status of two downstream targets of TSC2: 4E-BP1 and S6K. Our data suggest that, under particular physiologic or pathologic conditions, HtrA1 degrades TSC2 and activates the downstream targets. Considering that HtrA1 levels are significantly increased during embryogenesis, we speculate that one of the targets of HtrA1 activity during fetal development is the TSC2-TSC1 pathway.
Experimental investigation of magnetically actuated separation using tangential microfluidic channels and magnetic nanoparticles (2014)

A novel continuous switching/separation scheme of magnetic nanoparticles (MNPs) in a sub-microlitre fluid volume surrounded by neodymium permanent magnet is studied in this work using tangential microfluidic channels. Polydimethylsiloxane tangential microchannels are fabricated using a novel micromoulding technique that can be done without a clean room and at much lower cost and time. Negligible switching of MNPs is seen in the absence of magnetic field, whereas 90% of switching is observed in the presence of magnetic field. The flow rate of MNPs solution had dramatic impact on separation performance. An optimum value of the flow rate is found that resulted in providing effective MNP separation at much faster rate. Separation performance is also investigated for a mixture containing non-magnetic polystyrene particles and MNPs. It is found that MNPs preferentially moved from lower microchannel to upper microchannel resulting in efficient separation. The proof-of-concept experiments performed in this work demonstrates that microfluidic bioseparation can be efficiently achieved using functionalised MNPs, together with tangential microchannels, appropriate magnetic field strength and optimum flow rates. This work verifies that a simple low-cost magnetic switching scheme can be potentially of great utility for the separation and detection of biomolecules in microfluidic lab-on-a-chip systems.
Eine neue Methode zur Herstellung gemischter Disulfide. Vorläufige Mitteilung (1976)

A New Synthesis of Unsymmetrical Disulfides. A new synthesis of unsymmetrical disulfides 3a via alkylthio‐dialkyl‐sulfonium salts 6 is reported.
Immunoglobulin and T‐cell receptor gene rearrangement in Castleman's disease: molecular genetic analysis (2005)

The lymphoid cells in CD are most commonly polyclonal in origin, which supports a non-neoplastic origin. However, rare cases may show lymphocyte monoclonality, which could represent the development of a neoplastic population. The latter cases should be followed closely.
Inhaled Insulin Provides Improved Glycemic Control in Patients With Type 2 Diabetes Mellitus Inadequately Controlled With Oral Agents<subtitle>A Randomized Controlled Trial</subtitle> (2003)

The addition of preprandial INH to existing OHAs improves glycemic control without the need for injections in patients with type 2 diabetes mellitus failing to achieve satisfactory control with OHAs alone.
Understanding the Effect of Co3+ Substitution on the Electrochemical Properties of Lithium-Rich Layered Oxide Cathodes for Lithium-Ion Batteries (2014)

The effect of the substitution of Co3+ for equal amounts of Mn4+ and Ni2+ in the lithium-rich layered oxide cathode Li[Li0.2Ni0.2–x/2Mn0.6–x/2Cox]O2 (0 ≤ x ≤ 0.24) has been investigated systematically. Electrochemical charge/discharge measurements in lithium-ion cells indicate that the oxygen loss from the lattice during the first charge is a kinetically slow process, but it can be improved by the substitution of Co3+ due to an overlap of the Co3+/4+:t2g band with the top of the O2–:2p band and an increase in metal–oxygen covalence. Although the increased oxygen loss with increasing Co3+ substitution leads to a higher reversible capacity, the first cycle discharge voltage decreases slightly due to the higher concentration of Mn3+ ions created during the first discharge. However, both the capacity fade and the voltage decay increase during extended cycling with increasing Co3+ substitution due to an increasing migration of the transition-metal ions to the lithium layer, which is promoted by the higher number of oxide-ion vacancies in the lattice, to form a spinel-like phase.
Multimedia Systems, Standards, and Networks (2000)

Communication standards - Gotterdammerung? ITU H H.323 and H. 324 standards H.263 (including H.263+) and other ITU-T video coding standards overview of the MPEG standards a review of MPEG-4 general audio coding synthetic audio and SNHC audio in MPEG-4 MPEG-4 visual standard overview MPEG-4 natural video coding - part 1 MPEG-4 natural video coding - part 2 MPEG-4 texture coding MPEG-4 synthetic video MPEG-4 systems - overview MPEG-4 systems - elementary stream management and delivery MPEG-4 scene representation and interactivity Java in MPEG-4 (MPEG-J) MPEG-4 players implementation multimedia transport in ATM networks delivery and control of MPEG-4 content over IP networks multimedia over wireless multimedia search and retrieval image retrieval in digital laboratories MPEG-7 status and directions.
Hyaluronan (hyaluronic acid) and hyaluronectin in the extracellular matrix of human breast carcinomas: Comparison between invasive and non‐invasive areas (1992)

We performed quantitative determination of the distribution of hyaluronan (hyaluronic acid, HA) and the HA-binding protein, hyaluronectin (HN), 2 components of the extracellular matrix of tumor desmoplasia, within 71 human breast carcinomas. Results showed that HA and HN were more elevated in tumoral than in non-tumoral adjacent tissue, and that the peripheral invasive area of tumors contained increased levels of HA and HN as compared with the central non-invasive area (p less than 10(-3) and p less than 10(-5) respectively). HN and HA levels of 61 ductal carcinomas were related to the histological grade of tumors; no significant difference was found between grades for HA; HN was found to be significantly lower in grade III than in grade II tumors (p less than 0.01). HA and HN rates were correlated in grade I and grade II tumors and were not correlated in grade III. Mean percentage of HA saturation level by HN for whole tumors was found to be less than 4%, indicating that HA is essentially free of proteins and could be used as a target for cancer diagnosis or therapy.
Cell Activation and Apoptosis by Bacterial Lipoproteins Through Toll-like Receptor-2 (1999)

Apoptosis is implicated in the generation and resolution of inflammation in response to bacterial pathogens. All bacterial pathogens produce lipoproteins (BLPs), which trigger the innate immune response. BLPs were found to induce apoptosis in THP-1 monocytic cells through human Toll-like receptor-2 (hTLR2). BLPs also initiated apoptosis in an epithelial cell line transfected with hTLR2. In addition, BLPs stimulated nuclear factor-kappaB, a transcriptional activator of multiple host defense genes, and activated the respiratory burst through hTLR2. Thus, hTLR2 is a molecular link between microbial products, apoptosis, and host defense mechanisms.
Modulation of doxorubicin resistance in a doxorubicin-resistant human leukaemia cell by an immunoliposome targeting transferring receptor (1997)

Using a doxorubicin-resistant subline (K562/ADM) of human leukaemia K562 cells (Tsuruo et al, 1986), the effect of immunoliposomes that targeted a cellular transferrin receptor (TFR) was examined by neutralization of doxorubicin (DOX) resistance. OKT9-CIL, prepared by conjugation of DOX-encapsulated liposome with an anti-TFR monoclonal antibody, OKT9 (Aisenberg and Wilkes, 1980), showed similar binding to both K562 and K562/ADM. Although an 80-fold higher sensitivity to free DOX on cell growth inhibition in K562 than in K562/ADM was found, the difference was clearly diminished after OKT9-CIL treatment through the increased sensitivity of K562/ADM. The cellular DOX level 30 min after the exposure of free DOX was 45-fold lower in K562/ADM than in K562, whereas nearly equivalent DOX levels were detected in K562 and K562/ADM after OKT9-CIL treatment. In addition, DOX in K562/ADM in the free DOX treatment was efficiently excreted by 54% within 120 min of incubation, whereas almost all DOX supplied by OKT9-CIL remained uncleared. Fluorescence microscopic observation showed that OKT9-CIL was internalized into juxtanuclear vesicles in K562/ADM cells. These results suggest that OKT9-CIL has a potency to accumulate DOX, resulting in augmentation of DOX cytotoxicity in DOX-resistant tumour cells.