gebhart/patrepeval huggingface dataset:
Correlation of antiangiogenic and antitumor efficacy of N-biphenyl sulfonyl-phenylalanine hydroxiamic acid (BPHA), an orally-active, selective matrix metalloproteinase inhibitor. (1999)

The antiangiogenic activity and antitumor efficacy of a newly developed matrix metalloproteinase (MMP) inhibitor were examined. N-biphenyl sulfonyl-phenylalanine hydroxiamic acid (BPHA) potently inhibits MMP-2, -9, and -14, but not MMP-1, -3, or -7. In contrast, (-)BPHA, an enantiomer of BPHA, was inactive against all MMPs tested. Daily oral administration of 200 mg/kg BPHA, but not (-)BPHA in mice resulted in potent inhibition of tumor-induced angiogenesis, primary tumor growth, and liver metastasis. The growth inhibition activity of BPHA was 48% and 45% in a B16-BL6 melanoma and F2 hemangio-endothelioma model, respectively. BPHA also showed 42% inhibition of the liver metastasis of C-1H human colon carcinoma cells. These results indicate that selective MMP inhibition is correlated with antiangiogenic and antitumor efficacy and that the selective MMP inhibitor BPHA has therapeutic potential.
Overview of Extracellular Matrix (1998)

The extracellular matrix provides an environment for cells. It is produced, assembled, and modified by cells and in turn, it modifies the functions and behavior of the cells it encounters. The molecules that make up the matrix are diverse in both structure and function. This well-illustrated unit provides an introduction to the structure and function of the major components of the extracellular matrix and serves as a background for the other units in the chapter, which include protocols for isolation and analysis of individual components.
Antagonistic Effects of β-Site Amyloid Precursor Protein-cleaving Enzymes 1 and 2 on β-Amyloid Peptide Production in Cells (2003)

The deposition of extracellular beta-amyloid peptide (A beta) in the brain is a pathologic feature of Alzheimer's disease. The beta-site amyloid precursor protein cleaving enzyme 1 (BACE1), an integral membrane aspartyl protease responsible for cleavage of amyloid precursor protein (APP) at the beta-site, promotes A beta production. A second integral membrane aspartyl protease related to BACE1, referred to as beta-site amyloid precursor protein cleaving enzyme 2 (BACE2) has also been demonstrated to cleave APP at the beta-cleavage site in transfected cells. The role of endogenous BACE2 in A beta production remains unresolved. We investigated the role of endogenous BACE2 in A beta production in cells by selective inactivation of its transcripts using RNA interference. We are able to reduce steady state levels for mRNA for each enzyme by >85%, and protein amounts by 88-94% in cells. Selective inactivation of BACE1 by RNA interference results in decreased beta-cleaved secreted APP and A beta peptide secretion from cells, as expected. Selective inactivation of BACE2 by RNAi results in increased beta-cleaved secreted APP and A beta peptide secretion from cells. Simultaneous targeting of both enzymes by RNA interference does not have any net effect on A beta released from cells. Our observations of changes in APP metabolism and A beta are consistent with a role of BACE2 in suppressing A beta production in cells that co-express both enzymes.
JC‐1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess ΔΨ changes in intact cells: implications for studies on mitochondrial functionality during apoptosis (1997)

The sensitivity and specificity of three fluorescent probes used for cytofluorimetric analysis of mitochondrial membrane potential (delta psi) were studied in the U937 human cell line. First, the role of plasmamembrane in influencing the binding of the probes to mitochondria has been investigated. The depolarization of plasmamembrane with high doses of extracellular KCl had no immediate effects on the loading of JC-1, DiOC6(3) and rhodamine 123 (R123). However, after a few hours of culture in the presence of KCl, significant changes were observed only in cells stained with DiOC6(3). Second, a comparative study was performed concerning the effects of agents capable of collapsing deltapsi. While adding FCCP to cell cultures resulted in consistent changes in the fluorescence emission of both JC-1 and DiOC6(3) - but not of R123 - only cells stained with JC-1 responded to valinomycin. On the whole, our data indicate that JC-1 is a reliable probe for analyzing delta psi changes with flow cytometry, while the others show a lower sensitivity (R123), or a non-coherent behaviour, due to a high sensitivity to changes in plasmamembrane potential [DiOC6(3)]. These data cast some doubts on those studies that, using fluorescent probes that have a low sensitivity to delta psi, hypothesized that the fall in delta psi is one of the early events, if not one of the main causes, of apoptosis.
Mitochondrial Sequence Analysis for Forensic Identification Using Pyrosequencing Technology (2002)

Over recent years, requests for mtDNA analysis in the field of forensic medicine have notably increased, and the results of such analyses have proved to be very useful in forensic cases where nuclear DNA analysis cannot be performed. Traditionally, mtDNA has been analyzed by DNA sequencing of the two hypervariable regions, HVI and HVII, in the D-loop. DNA sequence analysis using the conventional Sanger sequencing is very robust but time consuming and labor intensive. By contrast, mtDNA analysis based on the pyrosequencing technology provides fast and accurate results from the human mtDNA present in many types of evidence materials in forensic casework. The assay has been developed to determine polymorphic sites in the mitochondrial D-loop as well as the coding region to further increase the discrimination power of mtDNA analysis. The pyrosequencing technology for analysis of mtDNA polymorphisms has been tested with regard to sensitivity, reproducibility, and success rate when applied to control samples and actual casework materials. The results show that the method is very accurate and sensitive; the results are easily interpreted and provide a high success rate on casework samples. The panel of pyrosequencing reactions for the mtDNA polymorphisms were chosen to result in an optimal discrimination power in relation to the number of bases determined.
Chromium and exercise training: effect on obese women (1997)

Chromium supplementation may affect various risk factors for coronary artery disease (CAD) and non-insulin-dependent diabetes mellitus (NIDDM), including body weight and composition, basal plasma hormone and substrate levels, and response to an oral glucose load. This study examined the effects of chromium supplementation (400 micrograms.d-1), with or without exercise training, on these risk factors in young, obese women. Chromium picolinate supplementation resulted in significant weight gain in this population, while exercise training combined with chromium nicotinate supplementation resulted in significant weight loss and lowered the insulin response to an oral glucose load. We conclude that high levels of chromium picolinate supplementation are contraindicated for weight loss in young, obese women. Moreover, our results suggest that exercise training combined with chromium nicotinate supplementation may be more beneficial than exercise training alone for modification of certain CAD and NIDDM risk factors.
Application of FMEA on the management of adverse nursing event in Obstetrics department (2012)

Objective To explore the effect of failure mode and effects analysis on the management of adverse nursing event in obstetrics department.Methods A total of 1 878 cases admitted to Gansu maternity and child-care hospital form July to December in 2010were assigned to the control group,2 016 cases from January to June were assigned to the observation group,Routine nursing was delivered to the control group,while failure mode and effect analysis additionally was applied to the observation group.The high risk factors of adverse nursing event and the high risk work process of nursing were prospectively analyzed,assessed and the RPN was figured out,then a priority action plan and interventions were implemented.Results The RPN of failure mode in the observation group was significantly lower than that in the control group (P ＜ 0.05) ; 67 adverse nursing events were reported in the observation group while 135 cases in the control group,and the difference was statistically significant difference (P ＜ 0.05 ).Conclusions The application of failure mode and effect analysis on adverse nursing events can improve the prediction and activeness.It also can decrease the incidence of adverse nursing events and improve medical safety.

Key words: 
Failure mode and effect analysis; Obstetrics; Adverse nursing event; Management
Myostatin Signals through a Transforming Growth Factor β-Like Signaling Pathway To Block Adipogenesis (2003)

Myostatin, a transforming growth factor beta (TGF-beta) family member, is a potent negative regulator of skeletal muscle growth. In this study we characterized the myostatin signal transduction pathway and examined its effect on bone morphogenetic protein (BMP)-induced adipogenesis. While both BMP7 and BMP2 activated transcription from the BMP-responsive I-BRE-Lux reporter and induced adipogenic differentiation, myostatin inhibited BMP7- but not BMP2-mediated responses. To dissect the molecular mechanism of this antagonism, we characterized the myostatin signal transduction pathway. We showed that myostatin binds the type II Ser/Thr kinase receptor. ActRIIB, and then partners with a type I receptor, either activin receptor-like kinase 4 (ALK4 or ActRIB) or ALK5 (TbetaRI), to induce phosphorylation of Smad2/Smad3 and activate a TGF-beta-like signaling pathway. We demonstrated that myostatin prevents BMP7 but not BMP2 binding to its receptors and that BMP7-induced heteromeric receptor complex formation is blocked by competition for the common type II receptor, ActRIIB. Thus, our results reveal a strikingly specific antagonism of BMP7-mediated processes by myostatin and suggest that myostatin is an important regulator of adipogenesis.
干旱、人类活动与火山灰农业——史前短期文化—生态变迁研究 (1993)

背景伍帕特基国家保护区(图一)所在乡野,依任何标准看都是干旱地区。这里的植被群是沙漠草地和沙漠灌木丛(Brown 1982:115—21;Turner 1982:145—55),基底是曾经折叠和断层、已丧失原始状态的多孔砂岩和石灰岩(Strahler 1944;Sehroeder 1977:4—5)。由于风大、年蒸发率(140cm)高、湿度相当低,水份散失极快,多孔基
MicroRNAs (2008)

MicroRNAs (miRNAs) are small, noncoding RNAs with regulatory functions, which play an important role in many human diseases, including cancer. An emerging number of studies show that miRNAs can act either as oncogenes or as tumor suppressor genes or sometimes as both. Germline, somatic mutations and polymorphisms can contribute to cancer predisposition. miRNA expression levels have diagnostic and prognostic implications, and their roles as anticancer therapeutic agents is promising and currently under investigation.
High-Speed Separation of Antisense Oligonucleotides on a Micromachined Capillary Electrophoresis Device (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHigh-Speed Separation of Antisense Oligonucleotides on a Micromachined Capillary Electrophoresis DeviceCarlo S. Effenhauser, Aran. Paulus, Andreas. Manz, and H. Michael. WidmerCite this: Anal. Chem. 1994, 66, 18, 2949–2953Publication Date (Print):September 15, 1994Publication History Published online1 May 2002Published inissue 15 September 1994https://pubs.acs.org/doi/10.1021/ac00090a024https://doi.org/10.1021/ac00090a024research-articleACS PublicationsRequest reuse permissionsArticle Views640Altmetric-Citations277LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Vesicular stomatitis virus G proteins with altered glycosylation sites display temperature-sensitive intracellular transport and are subject to aberrant intermolecular disulfide bonding. (1988)

In this report we have extended our studies on a panel of vesicular stomatitis virus G proteins with altered glycosylation sites. These mutant proteins were generated by oligonucleotide-directed mutagenesis of the coding sequence to create new consensus sites for asparagine-linked oligosaccharide addition. We report that the intracellular transport of most of the mutant proteins is temperature-sensitive, implying a polypeptide folding step is affected. In addition, we find that the nonglycosylated G protein and those mutant proteins which lack oligosaccharides at the normal positions are subject to aberrant intermolecular disulfide bonding, leading to the accumulation of large complexes in the endoplasmic reticulum. These results imply that carbohydrate plays an indirect role in the intracellular transport of G protein.
Diffuse large B-cell lymphoma with renal involvement: Outcome and risk of central nervous system relapse (2008)

8580 Background: Renal involvement is uncommon in diffuse large B-cell lymphoma. In a recent study, our group found that renal involvement was an independent risk factor for central nervous system (CNS) relapse (Villa, ASCO 2007). We reviewed the clinical features, risk of CNS involvement, and survival for an expanded cohort of patients. Methods: All patients with DLBCL and renal extranodal involvement diagnosed from January 1, 1982 to August 1, 2007 at the British Columbia Cancer Agency were retrospectively identified in the Lymphoid Cancer Database. Patients were included if they were >16 years old, had advanced stage (stage III /IV, or stage I /II with B symptoms or bulky (>10cm)) disease, were treated with curative intent and were free of CNS involvement at diagnosis. CNS relapse was diagnosed by CSF cytology, radiology or clinically. Results: There were 46(2%) patients with DLBCL with renal involvement at diagnosis and the following characteristics n(%): M:F 2.3:1; median age 59 y; 19(41) had bilateral renal involvement; 42(91) had stage IV disease; 43(94) IPI 3,4 or 5; 7(15) had GFR <30mL/min; 39(85) had high LDH. Nine(20) patients received CHOP plus rituximab (CHOPR), 27(59) received CHOP-like regimens and only 8(17) received intrathecal prophylaxis. In total, 15 patients (33%) have had a CNS relapse (leptomeninges 9; brain parenchyma 5; both 1). The median time to CNS relapse was 5.6 mos. There was no significant difference in presenting clinical features between those who had a CNS relapse and those who did not. The addition of rituximab did not reduce the risk of CNS relapse (6/19 32% CHOPR vs 9/27 33% CHOP-like, p=.90) or time to CNS relapse (p=.49). The addition of intrathecal chemotherapy was not protective. The 5y overall and progression-free survivals for the whole cohort were 29% and 28%, respectively, suggesting that salvage therapies are ineffective. Patients receiving CHOPR chemotherapy had improved 5y progression free (43% vs 18%, p=.12) and overall (49% vs 19%, p=.04) survival. Conclusion: There is an exceptionally high incidence of CNS relapse in patients with DLBCL and kidney involvement at diagnosis. The addition of rituximab appears to improve overall survival in this poor risk population, likely through improved control of systemic disease. No significant financial relationships to disclose.
Colored timed Petri-net and GA based approach to modeling and scheduling for wafer probe center (2003)

In this paper, we propose architecture to simulate wafer probe. We use a modeling tool named CTPN (colored-timed Petri nets) to model all testing flow. With CTPN model, we can predict the delivery date of any specific product under some scheduling policies efficiently and precisely. In the scheduling phase, we combine two popular methods to contract high-quality schedules. One is to select machines for lots and the other is to select lots for machines. In each method, we use the GA-based approach to search for the optimal combination of a number of heuristic rules. This CTPN-based GA scheduler and helps us to find the good solution so as to meet the requirements in the complicated environment.
Mercury vapor detection with a self-sensing, resonating piezoelectric cantilever (2003)

A microcantilever with an integrated piezoelectric film is demonstrated as a mercury vapor detector. The cantilever is self-sensing and self-actuating, and therefore does not need alignment of an external, optical detection system. This gives the new sensor system an advantage in array applications. Mercury vapor, when adsorbed onto gold on the cantilever, causes the stiffness, and therefore the natural frequency, of the cantilever to increase as a result of mercury gold amalgamation. This shift is detected using the piezoelectric portion of the cantilever in conjunction with a bridge circuit and amplifier. A mercury concentration of 93 ppb in nitrogen is detected.
Handsfree Decision Support: Toward a Non-invasive Human-Computer Interface*. (1995)

We describe our efforts to develop and test a prototype handsfree human–computer interface that allows healthcare personnel to access computer-based records and decision support without interfering with their daily clinical interactions and procedures. The methods are based on the integration of a computer-based decision-theoretic diagnostic system, speaker-independent speech-understanding, and a head-mounted, transmissive personal display that has the ability to embed translucent computer-based data, images, and inference results in a user’s field of view. The handsfree techniques highlight opportunities for user interfaces that can provide real-time and interactive decision support with minimal changes to existing patterns of clinical activity. We review the components of the system and describe the system’s performance and limitations. We describe methods for enhancing speech understanding for noisy environments by coupling the output of the Bayesian inference procedures with user models to provide methods that dynamically influence the language models used in speech recognition. Finally, we present several areas of ongoing and future research and development.
A new probe for super-resolution imaging of membranes elucidates trafficking pathways (2014)

The molecular composition of the organelles involved in membrane recycling is difficult to establish as a result of the absence of suitable labeling tools. We introduce in this paper a novel probe, named membrane-binding fluorophore-cysteine-lysine-palmitoyl group (mCLING), which labels the plasma membrane and is taken up during endocytosis. It remains attached to membranes after fixation and permeabilization and can therefore be used in combination with immunostaining and super-resolution microscopy. We applied mCLING to mammalian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromuscular junctions, and mammalian tissue. mCLING enabled us to study the molecular composition of different trafficking organelles. We used it to address several questions related to synaptic vesicle recycling in the auditory inner hair cells from the organ of Corti and to investigate molecular differences between synaptic vesicles that recycle actively or spontaneously in cultured neurons. We conclude that mCLING enables the investigation of trafficking membranes in a broad range of preparations.
N-terminal Peptides of Stromal Cell-derived Factor-1 with CXC Chemokine Receptor 4 Agonist and Antagonist Activities (1998)

Peptides corresponding to the N-terminal 9 residues of stromal cell-derived factor-1 (SDF-1) have SDF-1 activity. SDF-1, 1-8, 1-9, 1-9 dimer, and 1-17 induced intracellular calcium and chemotaxis in T lymphocytes and CEM cells and bound to CXC chemokine receptor 4 (CXCR4). The peptides had similar activities to SDF-1 but were less potent. Whereas native SDF-1 had half-maximal chemoattractant activity at 5 nM, the 1-9 dimer required 500 nM and was therefore 100-fold less potent. The 1-17 and a 1-9 monomer analog were 4- and 36-fold, respectively, less potent than the 1-9 dimer. Both the chemotactic and calcium response of the 1-9 dimer was inhibited by an antibody to CXCR4. The basis for the enhanced activity of the dimer form of SDF-1, 1-9 is uncertain, but it could involve an additional fortuitous binding site on the 1-9 peptide in addition to the normal SDF-1, 1-9 site. A 1-9 analog, 1-9[P2G] dimer, was found to be a CXCR4 antagonist. Overall this study shows that the N-terminal peptides are CXCR4 agonists or antagonists, and these could be leads for high affinity ligands.
Towards poly(ester) nanoparticles: recent advances in the synthesis of functional poly(ester)s by ring-opening polymerization (2010)

The recent trends in the synthesis of functional poly(ester)s by ring-opening polymerization (ROP) are reviewed. While the use of ROP processes for the synthesis of poly(lactide), poly(lactide-co-glycolide) and several poly(lactone)s has been well studied, the paucity of functional groups available for further reaction limits their application. Recent efforts to expand this available functionality are reviewed focusing on the application of renewable resources in the synthesis of new monomers and the utilization of click chemistry to provide common intermediate polymers in the manipulation of poly(ester) functionality. In turn these advances are leading to a new generation of precisely controlled nanoparticles comprised entirely of poly(ester)s.
Folding of an all-beta protein: independent domain folding in gamma II-crystallin from calf eye lens. (1990)

gamma II-crystallin from calf eye lens consists of two homologous domains, each composed of two similar "Greek key" motifs. As a consequence of the bilobal structure, a biphasic transition is seen upon unfolding by urea at low pH (monitored by circular dichroism, fluorescence emission, and ultracentrifugal analysis). In 3.3 +/- 0.5 M urea, a stable intermediate is formed at equilibrium, whereas 5.5 M urea causes maximum denaturation. Unfolding/folding kinetics display a complex pattern characterized by two kinetic phases. Both reactions exhibit strong dependence on the urea concentration; in the range of the respective transition, their rates are extremely slow (k approximately 1 x 10(-4)s-1). The kinetic mechanism of unfolding and refolding may be described by a three-state model: native in equilibrium intermediate in equilibrium denatured. The rate-determining steps are domain folding rather than domain pairing or proline isomerization. Kinetic analysis of the unfolding/folding of the intermediate populated in 3.0 M urea, pH 2.0, reveals that the kinetic and the equilibrium intermediates have similar structures. Limited proteolysis of gamma II-crystallin by pepsin in 3 M urea, pH 2, allows the NH2-terminal domain of the protein to be isolated. Unfolding/refolding of the fragment parallels the second transition in the above scheme, thus proving that the intermediate contains the COOH-terminal domain in its random state, whereas the NH2-terminal domain is still in its native conformation. In conclusion, folding of gamma II-crystallin proceeds through the independent sequential structuring of the domains.