gebhart/patrepeval huggingface dataset:
Syntactic segmentation and labeling of digitized pages from technical journals (1993)

A method for extracting alternating horizontal and vertical projection profiles are from nested sub-blocks of scanned page images of technical documents is discussed. The thresholded profile strings are parsed using the compiler utilities Lex and Yacc. The significant document components are demarcated and identified by the recursive application of block grammars. Backtracking for error recovery and branch and bound for maximum-area labeling are implemented with Unix Shell programs. Results of the segmentation and labeling process are stored in a labeled x-y tree. It is shown that families of technical documents that share the same layout conventions can be readily analyzed. Results from experiments in which more than 20 types of document entities were identified in sample pages from two journals are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
The Mollring Cutter™ Remote Endarterectomy: Preliminary Experience with a New Endovascular Technique for Treatment of Occlusive Superficial Femoral Artery Disease (1995)

Purpose: To evaluate the feasibility and efficacy of the Mollring Cutter™ remote endarterectomy (EA) technique in the treatment of occlusive superficial femoral artery (SFA) disease. Methods: A new device was developed to perform remote EA of occluded SFAs through a single groin incision. The technique initially uses a conventional ring stripper, which is exchanged for a double-ring cutter that transects the distal atheroma core. The entire core and cutter are removed simultaneously, and a Palmaz stent is implanted to secure the distal intimal flap. Results: Twenty-six consecutive patients with disabling claudication or critical ischemia were treated for long segment occlusions (n = 23) and multiple stenoses (n = 3). Patients were evaluated on the intention-to-treat basis. Clinical and anatomic success was achieved in all 26 SFAs. Average length of the endarterectomized segments was 29.9 cm (range 23 to 41). Technical problems were encountered intraoperatively in four patients, including one who required a second incision at the distal SFA to retrieve a broken ring. Recovery was uneventful in all patients. At up to 6-month follow-up, one reocclusion has been seen at 5 months. Conclusions: Based on this early experience, remote EA with the Mollring Cutter appears to be a feasible and effective method for treating occlusive SFA disease. Obviating the need for a second incision at knee level, this technique offers a less invasive approach that should facilitate postoperative recovery and earlier discharge.
Cryolipolysis™ for subcutaneous fat layer reduction (2009)

Although the mechanism of action for cryolipolysis is not yet completely understood, the efficacy and safety of this non-invasive procedure for fat layer reduction has been demonstrated in the studies available to data. Further studies willl assist in identifying the mechanism and elucidate the full potential of this technology to perform safe, non-invasive fat reduction for areas of local fat accumulation.
Effect of Fiber Diameter and Alignment of Electrospun Polyurethane Meshes on Mesenchymal Progenitor Cells (2009)

Effective strategies to guide cell alignment and the deposition of an oriented extracellular matrix are critical for the development of anisotropic engineered tissues suitable for the repair of ligament defects. Electrospinning is a promising means to create meshes that can align adherent cells, but the effect of fiber mesh architecture on differentiation has not been examined closely. Therefore, the goal of this study was to determine the effect of fiber diameter and the degree of fiber alignment on mesenchymal progenitor cell morphology, proliferation, and ligament gene expression. Specifically, a poly(ester urethane)urea elastomer was electrospun onto rigid supports under conditions designed to independently vary the mean fiber diameter (from 0.28 to 2.3 microm) and the degree of fiber alignment. Bone marrow stromal cells--seeded onto supported meshes--adhered to and proliferated on all surfaces. Cells assumed a more spindle-shaped morphology with increasing fiber diameter and degree of fiber alignment, and oriented parallel to fibers on aligned meshes. Expression of the ligament markers collagen 1alpha1, decorin, and tenomodulin appeared to be sensitive to fiber diameter and greatest on the smallest fibers. Concurrently, expression of the transcription factor scleraxis appeared to decrease with increasing fiber alignment. These results suggest that the formation of a ligament-like tissue on electrospun scaffolds is enhanced when the scaffolds consist of aligned submicron fibers.
Optical parametric oscillation in quasi-phase-matched GaAs (2004)

We demonstrate an optical parametric oscillator (OPO) based on GaAs. The OPO utilizes an all-epitaxially-grown orientation-patterned GaAs crystal that is 0.5 mm thick, 5 mm wide, and 11 mm long, with a domain reversal period of 61.2 microm. Tuning either the near-IR pump wavelength between 1.8 and 2 microm or the temperature of the GaAs crystal allows the mid-IR output to be tuned between 2.28 and 9.14 microm, which is limited only by the spectral range of the OPO mirrors. The pump threshold of the singly resonant OPO is 16 microJ for the 6-ns pump pulses, and the photon conversion slope efficiency reaches 54%. We also show experimentally the possibility of pump-polarization-independent frequency conversion in GaAs.
Immunochemistry of mammalian cholinesterases. (1986)

Advances in the study of cholinesterase biology have been facilitated by the development of polyclonal and monoclonal antibodies to acetylcholinesterase (AChE) (EC 3.1.1.7) and butyrylcholinesterase (BuChE) (EC 3.1.1.8) in several laboratories. Our work has focused on murine monoclonal antibodies to the mammalian enzymes. Two dozen antibodies are now in hand, with primary specificity for the AChE of human red blood cells, rabbit brain, and rat brain, and for the BuChE of human plasma. These antibodies exhibit a restricted but useful range of affinities for other mammalian cholinesterases of corresponding types. Several applications are described, including an analysis of BuChE phylogeny within the higher primates, an immunodisplacement assay of AChE in normal human red blood cells and cells from patients with paroxysmal nocturnal hemoglobinuria, a study of immunochemical differences between membrane-associated and soluble AChE of rabbit brain, and initial work on the immunofluorescence cytochemistry of the rat brain.
Effect of radiation sterilization and aging on ultrahigh molecular weight polyethylene (1981)

The mechanical properties of polyethylene components used in prosthesis are altered after fabrication by the sterilization procedure and by the environmental and mechanical aging which occurs after implantation. To assess the importance and extent of these alterations, ultrahigh molecular weight polyethylenes from two sources, Hercules 1900 and RCH 1000C, were subjected to gamma-ray irradiation and aging in serum and argon environments. Changes induced by these treatments in the structural and mechanical parameters have been determined by a variety of experimental techniques. The effect of irradiation is to introduce crosslinks and to increase the degree of crystallinity resulting in changes in the tensile properties in the direction of higher stiffness and reduced ductility. Aging for six months produced similar changes with subtle differences. The results suggest that the initially low degree of crystallinity which results from the sluggish mobility of the very long chain molecules offers the opportunity for significant changes in the properties during use in the long run.
Designing a Polycationic Probe for Simultaneous Enrichment and Detection of MicroRNAs in a Nanopore (2013)

The nanopore sensor can detect cancer-derived nucleic acid biomarkers such as microRNAs (miRNAs), providing a noninvasive tool potentially useful in medical diagnostics. However, the nanopore-based detection of these biomarkers remains confounded by the presence of numerous other nucleic acid species found in biofluid extracts. Their nonspecific interactions with the nanopore inevitably contaminate the target signals, reducing the detection accuracy. Here we report a novel method that utilizes a polycationic peptide-PNA probe as the carrier for selective miRNA detection in the nucleic acid mixture. The cationic probe hybridized with microRNA forms a dipole complex, which can be captured by the pore using a voltage polarity that is opposite the polarity used to capture negatively charged nucleic acids. As a result, nontarget species are driven away from the pore opening, and the target miRNA can be detected accurately without interference. In addition, we demonstrate that the PNA probe enables accurate discrimination of miRNAs with single-nucleotide difference. This highly sensitive and selective nanodielectrophoresis approach can be applied to the detection of clinically relevant nucleic acid fragments in complex samples.
Direct Visualization of HIV-1 Replication Intermediates Shows that Capsid and CPSF6 Modulate HIV-1 Intra-nuclear Invasion and Integration (2015)

Direct visualization of HIV-1 replication would improve our understanding of the viral life cycle. We adapted established technology and reagents to develop an imaging approach, ViewHIV, which allows evaluation of early HIV-1 replication intermediates, from reverse transcription to integration. These methods permit the simultaneous evaluation of both the capsid protein (CA) and viral DNA genome (vDNA) components of HIV-1 in both the cytosol and nuclei of single cells. ViewHIV is relatively rapid, uses readily available reagents in combination with standard confocal microscopy, and can be done with virtually any HIV-1 strain and permissive cell lines or primary cells. Using ViewHIV, we find that CA enters the nucleus and associates with vDNA in both transformed and primary cells. We also find that CA's interaction with the host polyadenylation factor, CPSF6, enhances nuclear entry and potentiates HIV-1's depth of nuclear invasion, potentially aiding the virus's integration into gene-dense regions.
Synthesis of 2-quinazolinonyl imidazolidinones (2003)

2-Chloromethyl-3-methylquinazolin-4(3H)-one 1 is converted to the azide 4 which easily undergoes cyclisation to give 2-quinazolinonyl imidazolidinones 5. The azides, however, yield 2,3-dimethylquinazolin-4(3H)-one 6 andlor 2-aryl- aminomethyl-3-methylquinazolin-4(3H)-one 2 under thermal, microwave and in acidic medium.
In vitro modulation of tumor progression-associated properties of hormone refractory prostate carcinoma cell lines by cytokines (1996)

Cytokine treatment can effectively alter several prostate carcinoma properties that are closely associated with tumor invasion and a metastatic phenotype, suggesting that immunotherapy via the local delivery of cytokines may have a potentially therapeutic role in the treatment of hormone-refractory prostate cancer through both direct and indirect antitumor mechanisms.
Free-Locomotion of Underwater Vehicles Actuated by Ionic Polymer Metal Composites (2009)

In this paper, we develop a modeling framework for studying free-locomotion of biomimetic underwater vehicles propelled by vibrating ionic polymer metal composites (IPMCs). The motion of the vehicle body is described using rigid body dynamics in fluid environments. Hydrodynamic effects, such as added mass and damping, are included in the model to enable a thorough description of the vehicle's surge, sway, and yaw motions. The time-varying actions exerted by the vibrating IPMC on the vehicle body, including thrust, lift, and moment, are estimated by combining force and vibration measurements with reduced order modeling based on modal analysis. The model predictions are validated through experimental results on a miniature remotely controlled fish-like robotic swimmer.
The Influence of Laboratory Aging Method on the Rheological Properties of Asphalt Binders (2002)

Abstract Asphalt binders used in asphalt concrete roadway pavements experience aging during construction and subsequently during their service lives. Aging is the combined effect of the evaporation of volatile compounds and the chemical reaction of residual compounds with oxygen. This aging affects the rheological properties of asphalt binders. The SuperpaveTM testing and specification system uses two laboratory procedures for aging of binders prior to measuring their rheological properties, namely the rolling thin film oven (RTFO) and the pressure aging vessel (PAV). These two procedures are used to simulate the aging that takes place during construction and during service, respectively. This paper examines whether the SuperpaveTM prescribed sequence of binder aging procedures (i.e., the RTFO followed by the PAV) is necessary, or whether similar binder rheological properties are obtained using the PAV procedure only. For this purpose, three binders were tested, namely an unmodified PG 64-28, an SBS polymer-modified binder of the same grade, and an SBS polymer-modified PG 76-28. The low temperature and fatigue rheological properties were measured by a bending beam rheometer (BBR) and a dynamic shear rheometer (DSR), respectively. The findings of the study suggest that, with a few exceptions, the rheological properties measured after aging with the RTFO followed by PAV are significantly different than those obtained after PAV aging only.
Tumor suppression by autophagy through the management of metabolic stress (2008)

Autophagy plays a critical protective role maintaining energy homeostasis and protein and organelle quality control. These functions are particularly important in times of metabolic stress and in cells with high energy demand such as cancer cells. In emerging cancer cells, autophagy defect may cause failure of energy homeostasis and protein and organelle quality control, leading to the accumulation of cellular damage in metabolic stress. Some manifestations of this damage, such as activation of the DNA damage response and generation of genome instability may promote tumor initiation and drive cell-autonomous tumor progression. In addition, in solid tumors, autophagy localizes to regions that are metabolically stressed. Defects in autophagy impair the survival of tumor cells in these areas, which is associated with increased cell death and inflammation. The cytokine response from inflammation may promote tumor growth and accelerate cell non-autonomous tumor progression. The overreaching theme is that autophagy protects cells from damage accumulation under conditions of metabolic stress allowing efficient tolerance and recovery from stress, and that this is a critical and novel tumor suppression mechanism. The challenge now is to define the precise aspects of autophagy, including energy homeostasis and protein and organelle turnover, that are required for the proper management of metabolic stress that suppress tumorigenesis. Furthermore, we need to be able to identify human tumors with deficient autophagy, and to develop rational cancer therapies that take advantage of the altered metabolic state and stress responses inherent to this autophagy defect.
Galectin-3 in Cardiac Remodeling and Heart Failure (2010)

Galectin-3 is a member of the galectin family, which consists of animal lectins that bind beta-galactosides. Recently, a role for galectin-3 in the pathophysiology of heart failure has been suggested. It was observed that galectin-3 is specifically upregulated in decompensated heart failure compared with compensated heart failure in animal models of heart failure. This has been associated with activation of fibroblasts and macrophages, which are a hallmark of cardiac remodeling. Therefore, galectin-3 may be a culprit biomarker in heart failure. Initial clinical observations indicate that galectin-3 may be a useful biomarker for decompensated heart failure, with incremental value over well-used "pressure-dependent" biomarkers, such as B-type natriuretic peptide. Future studies should focus on galectin-3 biology to better address the usefulness of galectin-3 as a biomarker and probe the usefulness of anti-galectin-3 therapy in treating heart failure.
The Localization and Activity of Sphingosine Kinase 1 Are Coordinately Regulated with Actin Cytoskeletal Dynamics in Macrophages (2007)

The physiologic and pathologic functions of sphingosine kinase (SK) require translocation to specific membrane compartments. We tested the hypothesis that interactions with actin filaments regulate the localization of SK1 to membrane surfaces, including the plasma membrane and phagosome. Macrophage activation is accompanied by a marked increase in association of SK1 with actin filaments. Catalytically-inactive (CI)- and phosphorylation-defective (PD)-SK1 mutants exhibited reductions in plasma membrane translocation, colocalization with cortical actin filaments, membrane ruffling, and lamellipodia formation, compared with wild-type (WT)-SK1. However, translocation of CI- and PD-SK1 to phagosomes were equivalent to WT-SK1. SK1 exhibited constitutive- and stimulus-enhanced association with actin filaments and F-actin-enriched membrane fractions in both intact macrophages and a novel in vitro assay. In contrast, SK1 bound G-actin only under stimulated conditions. Actin inhibitors disrupted SK1 localization and modulated its activity. Conversely, reduction of SK1 levels or activity via RNA interference or specific chemical inhibition resulted in dysregulation of actin filaments. Thus, the localization and activity of SK1 are coordinately regulated with actin dynamics during macrophage activation.
Prevalent mutations in prostate cancer (2005)

Quantitative and structural genetic alterations cause the development and progression of prostate cancer. A number of genes have been implicated in prostate cancer by genetic alterations and functional consequences of the genetic alterations. These include the ELAC2 (HPC2), MSR1, and RNASEL (HPC1) genes that have germline mutations in familial prostate cancer; AR, ATBF1, EPHB2 (ERK), KLF6, mitochondria DNA, p53, PTEN, and RAS that have somatic mutations in sporadic prostate cancer; AR, BRCA1, BRCA2, CHEK2 (RAD53), CYP17, CYP1B1, CYP3A4, GSTM1, GSTP1, GSTT1, PON1, SRD5A2, and VDR that have germline genetic variants associated with either hereditary and/or sporadic prostate cancer; and ANXA7 (ANX7), KLF5, NKX3-1 (NKX3.1), CDKN1B (p27), and MYC that have genomic copy number changes affecting gene function. More genes relevant to prostate cancer remain to be identified in each of these gene groups. For the genes that have been identified, most need additional genetic, functional, and/or biochemical examination. Identification and characterization of these genes will be a key step for improving the detection and treatment of prostate cancer.
The Cytological Localization of Erythropoietin in the Human Kidney Using the Fluorescent Antibody Technique (1971)

Intense fluorescent staining of cells in the glomerular tuft has been demonstrated in an anemic human kidney following application of an antibody to erythropoietin using the indirect fluorescent antibody technique. The fluorescence was more localized in the cytoplasm of epithelial cells in the periphery of the glomerular tuft. A slight amount of fluorescent staining in the glomeruli was blocked by a highly purified preparation of human urinary erythropoietin. The present studies suggest that the visceral epithelial cell of the glomerular tuft is the most likely source of erythropoietin.
Arms and the physicist (1995)

The essays in this volume present Herbert York's historical and political analyses of the major events of the arms race. York is the former director of research and engineering at the Defence Department, former chief U.S. negotiator for the Comprehensive Test Ban Treaty, and former director of Lawrence Livermore National Laboratory. Reflecting on his wide-ranging career and involvement in national security and the role of nuclear technology. Historians of science and physics, political scientists, and interested lay readers.
Cooperative Avoidance Control for Multiagent Systems (2007)

The objective of this paper is to present a methodology for designing cooperative control laws for individual agents that guarantee collision avoidance in multiagent systems. The proposed avoidance control laws are easy to design and implement, and may be directly appended to the optimal control laws of the individual agents within the cooperation framework. The avoidance control laws are computed using value functions that resemble the behavior of barrier functions in the static optimization theory. The most attractive feature of the proposed optimization scheme is the fact that the avoidance laws are active only in the bounded sensing regions of each individual agent, and they do not interfere with the agents’ individual optimal control laws outside of these regions.
New Sandwich‐Type Enzyme‐Linked Immunosorbent Assay for Human MxA Protein in a Whole Blood Using Monoclonal Antibodies Against GTP‐Binding Domain for Recognition of Viral Infection (2012)

We have developed a new ELISA for measuring MxA protein in human whole blood using mAbs specific for the GTP-binding domain of MxA. This ELISA has analytical performance enough for routine clinical assay and can be used in detecting the possibility of viral infection.
Low‐Temperature Wafer Bonding of Surfaces Using a Reactive Sputtered Oxide (1994)

A low‐temperature silicon wafer bonding technique has been demonstrated to give bond strengths as high as 2.4 MPa for anneal temperatures as low as 400°C. The technique involves RF magnetron reactive sputter deposition of nonstoichiometric films on one or both surfaces prior to contacting at room temperature. Surfaces investigated include bare silicon, thermally grown silicon dioxide, and low pressure chemical vapor deposited silicon nitride. Experimental results provide new evidence that removal of oxygen or water from the bonding interface during annealing is critical to the formation of strong interfacial bonds.