gebhart/patrepeval huggingface dataset:
A CMOS 6-Bit 16-GS/s Time-Interleaved ADC Using Digital Background Calibration Techniques (2011)

An 8-channel 6-bit 16-GS/s time-interleaved analog- to-digital converter (TI ADC) was fabricated using a 65 nm CMOS technology. Each analog-to-digital channel is a 6-bit flash ADC. Its comparators are latches without the preamplifiers. The input-referred offsets of the latches are reduced by digital offset calibration. The TI ADC includes a multi-phase clock generator that uses a delay-locked loop to generate 8 sampling clocks from a reference clock of the same frequency. The uniformity of the sampling intervals is ensured by digital timing-skew calibration. Both the offset calibration and the timing-skew calibration run continuously in the background. At 16 GS/s sampling rate, this ADC chip achieves a signal-to-distortion-plus-noise ratio (SNDR) of 30.8 dB. The chip consumes 435 mW from a 1.5 V supply. The ADC active area is 0.93 × 1.58 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> .
New Human CD22/Siglec‐2 Ligands with a Triazole Glycoside (2017)

CD22 is a member of the Siglec family. Considerable attention has been drawn to the design and synthesis of new Siglec ligands to explore target biology and innovative therapies. In particular, CD22-ligand-targeted nanoparticles with therapeutic functions have proved successful in preclinical settings for blood cancers, autoimmune diseases, and tolerance induction. Here we report the design, synthesis and affinity evaluation of a new class of Siglec ligands: namely sialic acid derivatives with a triazole moiety replacing the natural glycoside oxygen atom. In addition, we describe important and surprising differences in binding to CD22 expressed at the cell surface for compounds with distinct valences. The new class of compounds might serve as a template for the design of ligands for other members of the Siglec family and next-generation CD22-ligand-based targeted therapies.
Refractory Epilepsy: One Size Does Not Fit All (2006)

A unifying definition of refractory epilepsy has been hotly debated but, to date, has not been agreed upon. Evidence from clinical trials indicates that some patients actually are not refractory, as many will partially respond to add-on treatment or will worsen when antiepileptic drugs (AEDs) are removed. There are several important issues relating to the assessment of AED response that routinely have not been addressed in the determination of treatment responsiveness, such as incorporating baseline seizure severity, including partial response rather than solely an all-or-none response, and the consideration of variability in response over time.
The PowerPC Architecture: A Specification for a New Family of RISC Processors (1994)

This is the official technical description of the PowerPC architecture and its hardware conventions, developed jointly by IBM, Motorola, and Apple. The book is an essential reference for hardware and system-software designers and applications programmers developing a range of products using implementations of the PowerPC family of microprocessors-from palmtops to teraFLOPS. The PowerPC architecture provides a stable base for software, allowing applications that run on one PowerPC processor to run consistently on any other PowerPC processor. In addition, well-designed operating systems can be moved from one processor implementation to another by making only a few minor changes. To achieve this, the specification of the architecture has been structured into three Books, corresponding to a distinct level of the architecture: Book I, User Instruction Set Architecture, describes the registers, instructions, storage model, and execution model that are available to all application programs. Book II, Virtual Environment Architecture, describes features of the architecture that permit application programs to create or modify code, to share data among programs in a multiprocessing system, and to optimize the performance of storage accesses. Book III, Operating Environment Architecture, describes features of the architecture that permit operating systems to allocate and manage storage, to handle errors encountered by application programs, to support I/O devices, and to provide the other services expected of secure, modern multiprocessor operating systems. An important feature of these specifications is that they only constrain implementations on matters that affect software compatibility. Even more significant, they specify the architecture in a manner that is independent of implementation. The PowerPC Architecture is a must for anyone who needs to understand the levels of compatibility between different processors in the PowerPC family-the 601 microprocessor, the 603 (low-end, battery-powered requirements), 604 (optimized price/performance for scaleable symmetric multiprocessors), and the 620 (for high-end technical and commercial requirements about performance).
Directed Evolution of a Novel Adeno-associated Virus (AAV) Vector That Crosses the Seizure-compromised Blood–Brain Barrier (BBB) (2009)

DNA shuffling and directed evolution were employed to develop a novel adeno-associated virus (AAV) vector capable of crossing the seizure-compromised blood-brain barrier (BBB) and transducing cells in the brain. Capsid DNA from AAV serotypes 1-6, 8, and 9 were shuffled and recombined to create a library of chimeric AAVs. One day after kainic acid-induced limbic seizure activity in rats, the virus library was infused intravenously (i.v.), and 3 days later, neuron-rich cells were mechanically dissociated from seizure-sensitive brain sites, collected and viral DNA extracted. After three cycles of selection, green fluorescent protein (GFP)-packaged clones were administered directly into brain or i.v. 1 day after kainic acid-induced seizures. Several clones that were effective after intracranial administration did not transduce brain cells after the i.v. administration. However, two clones (32 and 83) transduced the cells after direct brain infusion and after i.v. administration transduced the cells that were localized to the piriform cortex and ventral hippocampus, areas exhibiting a seizure-compromised BBB. No transduction occurred in areas devoid of BBB compromise. Only one parental serotype (AAV8) exhibited a similar expression profile, but the biodistribution of 32 and 83 diverged dramatically from this parental serotype. Thus, novel AAV vectors have been created that can selectively cross the seizure-compromised BBB and transduce cells.
Cyclin-dependent kinase 5 activity regulates pain signaling (2006)

Several molecules and cellular pathways have been implicated in nociceptive signaling, but their precise molecular mechanisms have not been clearly defined. Cyclin-dependent kinase 5 (Cdk5) is a proline-directed serine/threonine kinase implicated in the development and disease of the mammalian nervous system. The precise role of this kinase in sensory pathways has not been well characterized. Here we report a molecular role for Cdk5 in nociception. We identified the expression of Cdk5 and its activator p35 in nociceptive neurons, which is modulated during a peripheral inflammatory response. Increased calpain activity in sensory neurons after inflammation resulted in the cleavage of p35 to p25, which forms a more stable complex with Cdk5 and, consequently, leads to elevation of Cdk5 activity. p35 knockout mice (p35(-/-)), which exhibit significantly decreased Cdk5 activity, showed delayed responses to painful thermal stimulation compared with WT controls. In contrast, mice overexpressing p35, which exhibit elevated levels of Cdk5 activity, were more sensitive to painful thermal stimuli than were controls. In conclusion, our data demonstrate a role for Cdk5/p35 activity in primary afferent nociceptive signaling, suggesting that Cdk5/p35 may be a target for the development of analgesic drugs.
Seasonal Affective Disorder (1984)

Seasonal affective disorder (SAD) is a syndrome characterized by recurrent depressions that occur annually at the same time each year. We describe 29 patients with SAD; most of them had a bipolar affective disorder, especially bipolar II, and their depressions were generally characterized by hypersomnia, overeating, and carbohydrate craving and seemed to respond to changes in climate and latitude. Sleep recordings in nine depressed patients confirmed the presence of hypersomnia and showed increased sleep latency and reduced slow-wave (delta) sleep. Preliminary studies in 11 patients suggest that extending the photoperiod with bright artificial light has an antidepressant effect.
Cancer Cell Cycles (1996)

Uncontrolled cell proliferation is the hallmark of cancer, and tumor cells have typically acquired damage to genes that directly regulate their cell cycles. Genetic alterations affecting p16(INK4a) and cyclin D1, proteins that govern phosphorylation of the retinoblastoma protein (RB) and control exit from the G1 phase of the cell cycle, are so frequent in human cancers that inactivation of this pathway may well be necessary for tumor development. Like the tumor suppressor protein p53, components of this "RB pathway," although not essential for the cell cycle per se, may participate in checkpoint functions that regulate homeostatic tissue renewal throughout life.
Analysis and design of 3 stage voltage rectifier multiplier and 2 stage multi-phase voltage doubler for and energy harvesting system (2012)

The reliability and the efficiency of the wireless link between the TX/RX for wireless sensors devices depends on the environmental conditions such as change in the physical distance or changes in the orientation between transmitter and receiver. So the need arises to monitor and the ability to adjust the wireless link between TX/RX without interrupting the operation of the wireless sensor. Also the ambient wireless energy can also be harvested to power the wireless sensor circuitry. We propose an N-Stage Voltage Multiplier/Rectifier built in AMI06 process using a Schottky diode to convert the ambient RF Energy into DC voltage which can be measured to evaluate the strength of the Wireless link. The proposed system can be used to monitoring and vary the wireless link parameters such as the resonant matching condition between TX/RX antenna coil and physical alignment without interrupting the operation of the wireless sensor. Also the DC energy harvested can be boosted further by our proposed multiphase charge pump to a higher DC Level which can be used by the wireless sensor circuitry. The DC power harvested can also be used alongside the on board battery which will lead to increase in battery efficiency. The voltage multiplier/rectifier and charge pump involving the power stage and the feedback circuitry can be built on the same die as the wireless sensor circuitry which can lead to less bulky system.
Robustness and Vulnerability of Scale-Free Random Graphs (2004)

Recently many new "scale-free" random graph models have been introduced, motivated by the power-law degree sequences observed in many large-scale, real-world networks. Perhaps the best known, the Barabási-Albert model, has been extensively studied from heuristic and experimental points of view. Here we consider mathematically two basic characteristics of a precise version of this model, the LCD model, namely robustness to random damage, and vulnerability to malicious attack. We show that the LCD graph is much more robust than classical random graphs with the same number of edges, but also more vulnerable to attack. In particular, if vertices of the _n_-vertex LCD graph are deleted at random, then as long as any positive proportion remains, the graph induced on the remaining vertices has a component of order _n_. In contrast, if the deleted vertices are chosen maliciously, a constant fraction less then 1 can be deleted to destroy all large components. For the Barabási-Albert model, these questions have been studied experimentally and heuristically by several groups.
The regulation of megakaryocyte and platelet production (1989)

Thrombopoietin, a hormone that regulates blood platelet production, is now recognized to be an important in vivo hematopoietic stimulator. In this concise review, the background on thrombopoietin, development of assays, and identification of sources of the hormone are summarized, along with brief descriptions of other controlling factors, sites of thrombopoietin production, results of producing antibodies against the factor, sites of action of thrombopoietin both in vitro and in vivo, and its effect on blood platelet production. Suitable assays and stable sources of thrombopoietin have now been identified and their development will permit production of recombinant material. Once the gene is cloned, it is expected that recombinant thrombopoietin will be invaluable for treating patients with several platelet production problems.
Early CDT-Lung: An Immunobiomarker Test as an Aid to Early Detection of Lung Cancer (2011)

Abstract Recent publications have reported the technical and clinical validation of EarlyCDT-Lung, an autoantibody test which detected elevated autoantibodies in 40% of lung cancers at diagnosis. This manuscript reports the results of EarlyCDT-Lung run on four new (postvalidation) data sets. The following four cohorts of patients (n = 574) with newly diagnosed lung cancer were identified: group 1 (n = 122), 100% small cell lung cancer (SCLC); group 2 (n = 249), 97% non-small cell lung cancer (NSCLC); group 3 (n = 122), 100% NSCLC; group 4 (n = 81), 62% NSCLC. Serum samples were obtained after diagnosis, prior to any anticancer treatment. Autoantibody levels were measured against a panel of six tumor-related antigens (p53, NY-ESO-1, CAGE, GBU4–5, Annexin 1, and SOX2) in the EarlyCDT-Lung panel and previously established cutoffs applied. In groups 2, 3, and 4, patients were individually matched by gender, age, and smoking history to a control individual with no history of malignant disease. Assay sensitivity was tested in relation to cancer type and stage, and in the matched normals to demographic variables. The autoantibody panel showed sensitivity/specificity of 57%/n.d (not done) for SCLC in group 1, 34%/87% for NSCLC in group 2, 31% and 84% for NSCLC in group 3, and 35%/89% for NSCLC and 43%/89% for SCLC in group 4. There was no significant difference in positivity of EarlyCDT-Lung and different lung cancer stages. These studies confirm the value of an autoantibody assay, EarlyCDT-Lung, as an aid to detecting lung cancer in patients at high risk of the disease. Cancer Prev Res; 4(7); 1126–34. ©2011 AACR.
Elevated Expression of the Apoptotic Regulator Mcl-1 at the Time of Leukemic Relapse (1998)

Bcl-2, Bcl-xL, and Mcl-1 are three related intracellular polypeptides that have been implicated as negative regulators of apoptosis. In contrast, the partner protein Bax acts as a positive regulator of apoptosis. Based on the observation that all four of these polypeptides are expressed in a variety of acute myelogenous leukemia (AML) and acute lymphocytic leukemia (ALL) cell lines, cellular levels of these polypeptides were examined by immunoblotting in bone marrow samples harvested from 123 adult AML patients and 36 adult ALL patients before initial antileukemic therapy. Levels of Bcl-2, Mcl-1, Bcl-xL, and Bax each varied over a more than 10-fold range in different pretreatment leukemia specimens. When the 54 AML and 23 ALL samples that contained greater than 80% malignant cells were examined in greater detail, it was observed that pretreatment levels of Bcl-2 and Mcl-1 correlated with each other (R = .44, P < .001 for AML and R = .79, P < .0001 for ALL). In addition, a weak negative correlation between Bax expression and age was observed in AML samples (R = -0.35, P < .02) but not ALL samples. There was no relationship between pretreatment levels of these polypeptides and response to initial therapy. However, examination of 19 paired samples (the first harvested before chemotherapy and the second harvested 23 to 290 days later at the time of leukemic recurrence) revealed a greater than or equal to twofold increase in Mcl-1 levels in 10 of 19 pairs (7 of 15 AML and 3 of 4 ALL) at recurrence. In contrast, 2 of 19 pairs contained twofold less Mcl-1 at the time of recurrence. Approximately equal numbers of samples showed twofold increases and decreases in Bcl-2 (5 increases, 3 decreases) and Bcl-xL (1 increase, 4 decreases) at recurrence. Bax levels did not show a twofold decrease in any patient. these results, coupled with recent observations that cells overexpressing Mcl-1 are resistant to a variety of chemotherapeutic agents, raise the possibility that some chemotherapeutic regimens might select for leukemia cells with elevated levels of this particular apoptosis inhibitor.
Efficient Delivery of Bioactive Antibodies into the Cytoplasm of Living Cells by Charge‐Conversional Polyion Complex Micelles (2010)

Nichts wie rein! Immunglobulin G (IgG) kann nach Ladungsumkehr und Behandlung mit einem kationischen Blockcopolymer in Form von Polyionkomplex(PIC)-Micellen in das Plasma lebender Zellen eingebracht werden (siehe Bild). In der Zelle erlangt IgG in Abhängigkeit vom pH-Wert seine biologische Wirkung zurück, sodass das Wachstum humaner Hepatomzellen durch IgG-Bindung an intrazelluläre Zielmoleküle gesteuert werden kann. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Synthesis of Functionalized 1,8‐Naphthyridinones and Their Evaluation as Novel, Orally Active CB1 Receptor Inverse Agonists. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients (2015)

Liquid biopsies come of age offering unexploited potential to monitor and react to tumor evolution. We developed a cost-effective assay to non-invasively determine the immune status of glioblastoma (GBM) patients. Employing newly developed printed peptide microarrays we assessed the B-cell response against tumor-associated antigens (TAAs) in 214 patients. Firstly, sera of long-term (36+ months, LTS, n=10) and short-term (6-10 months, STS, n=14) surviving patients were screened for prognostic antibodies against 1745 13-mer peptides covering known TAAs (TNC, EGFR, GLEA2, PHF3, FABP5, MAGEA3). Next, survival associations were investigated in two retrospective independent multicenter validation sets (n=61, n=129, all IDH1-wildtype). Reliability of measurements was tested using a second array technology (spotted arrays). LTS/STS screening analyses identified 106 differential antibody responses. Evaluating the Top30 peptides in validation set 1 revealed three prognostic peptides. Prediction of TNC peptide VCEDGFTGPDCAE was confirmed in a second set (p=0.043, HR=0.66 [0.44-0.99]) and was unrelated to TNC protein expression. Median signals of printed arrays correlated with pre-synthesized spotted microarrays (p<0.0002, R=0.33). Multiple survival analysis revealed independence of age, gender, KPI and MGMT status. We present a novel peptide microarray immune assay that identified increased anti-TNC VCEDGFTGPDCAE serum antibody titer as a promising non-invasive biomarker for prolonged survival.
Characterization of human tracheal epithelial cells transformed by an origin-defective simian virus 40. (1988)

To facilitate understanding of the mechanisms underlying pulmonary diseases, including lung cancer and cystic fibrosis, we have transformed and characterized cultures of human tracheal epithelial cells. Cells were transfected by calcium phosphate precipitation with a plasmid containing a replication-defective simian virus 40 (SV40) genome. Colonies of cells with enhanced growth potential were isolated and analyzed for transformation- and epithelial-specific characteristics. Precrisis cells were observed to express the SV40 large tumor antigen, produce cytokeratins, have microvilli, and form tight junctions. After crisis, cells continued to express the SV40 large tumor antigen as well as epithelial-specific cytokeratins and to display the apical membrane microvilli. Apical membrane Cl channels were opened in postcrisis cells exposed to 50 microM forskolin. These channels showed electrical properties similar to those observed in primary cultures. The postcrisis cells have been in culture for greater than 250 generations and are potentially "immortal." In addition to providing a useful in vitro model for the study of ion transport by human airway epithelial cells, the cells can be used to examine stages of neoplastic progression.
The First Formal Asymmetric Synthesis of Phorbol (1997)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTThe First Formal Asymmetric Synthesis of PhorbolPaul A. Wender, Kenneth D. Rice, and Mark E. SchnuteView Author Information Department of Chemistry, Stanford University Stanford, California 94305 Cite this: J. Am. Chem. Soc. 1997, 119, 33, 7897–7898Publication Date (Web):August 20, 1997Publication History Received26 February 1997Published online20 August 1997Published inissue 1 August 1997https://pubs.acs.org/doi/10.1021/ja9706256https://doi.org/10.1021/ja9706256rapid-communicationACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views8202Altmetric-Citations174LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Alcohols,Cyclization,Ethers,Oxidation,Stereoselectivity Get e-Alerts