gebhart/patrepeval huggingface dataset:
An Impaired Breeding Phenotype in Mice with a Genetic Deletion of Beta-2 Microglobulin and Diminished MHC Class I Expression: Role in Reproductive Fitness1 (2007)

Beta-2 microglobulin (B2M) plays a pivotal role in the biology of mammals, including its association with major histocompatibility complex (MHC) Class I gene products. The latter molecules have been shown to affect reproduction in both mice and humans, although the exact mechanism is still unknown. Here we report the results of a longitudinal study of the reproductive performance of a genetically modified B2m deficient mouse strain with low MHC Class I expression. Our data show that this mouse strain has an impaired reproductive performance. However, the mice superovulate well and show a normal estrous cycle. Breeding studies from crosses between the transgenic mice and the wild-type parental strain show that B2m deficient mice have a significantly lower frequency of mating than the control B2m+/+ mice. In addition, the litter size and weaning success of B2m deficient mice were lower than the control. Perinatal lethality of the B2m deficient offspring was also inflicted by cannibalism of the young pups by the B2m deficient female. The impaired breeding phenotype (IBP) can be reversed by reintroducing the B2m gene in F1 heterozygous B2m+/- animals; thus the presence of B2M confers a normal breeding pattern. The acquisition of an impaired breeding phenotype (IBP) as a result of the knockout of B2m directly implicates B2M in the reproductive cycle of mice and raises the possibility of an effect of B2M on the reproduction of other mammals.
Designing Large Triangular Chiral Macrocycles:  Efficient [3 + 3] Diamine−Dialdehyde Condensations Based on Conformational Bias (2000)

Triangular 30- and 27-membered hexaiminomacrocycles 4 and 5 of D(3) and C(3) symmetry, respectively, are readily obtained by unprecedented [3 + 3] cyclocondensation of (R,R)-1, 2-diaminocyclohexane with, accordingly, terephthalaldehyde and isophthalaldehyde. The course of the reaction, leading to macrocyclization, is governed by conformational constraints imposed on the structural components of the intermediate products, as shown by molecular modeling. X-ray analysis of cocrystal 4.AcOEt revealed that the macrocycle symmetry significantly departs from ideal D(3) symmetry due to crystal environment. Cyclic hexaamines 6 and 7 were prepared by sodium borohydride reduction of 4 and 5, respectively.
Characterisation of Acute Murine Dextran Sodium Sulphate Colitis: Cytokine Profile and Dose Dependency (2000)

Minor variations in fluid consumption do not affect the severity of DSS-induced injury in mice. The acute murine DSS colitis model is useful for studying the pathophysiological aspects of colonic inflammatory diseases as IBD and for evaluating new potential therapeutic agents
Hydrolyticmicroneedles as Transdermal Drug Delivery System (2007)

We developed hydrolytic microneedles as a transdermal drug delivery system to overcome the shortcomings of existing microneedle-systems: long-term stay of broken needles in the skin and high cost for safe disposal of used needles. The present microneedles spontaneously dissolve by hydrolysis in human skin as well as in the (boiled) water. We verified their basic utility through experiments.
Reconstitution of nucleotide excision nuclease with UvrA and UvrB proteins from Escherichia coli and UvrC protein from Bacillus subtilis. (1990)

Recently, an open reading frame which has a deduced amino acid sequence that shows 38% homology to Escherichia coli UvrC protein was found upstream of the aspartokinase II gene (ask) in Bacillus subtilis (Chen, N.-Y., Zhang, J.-J., and Paulus, H. (1989) J. Gen. Microbiol. 135, 2931-2940). We found that plasmids containing this open reading frame complement the uvrC mutations in E. coli. We joined the open reading frame to a tac promoter to amplify the gene product in E. coli and purified the protein to near homogeneity. The apparent molecular weight of the gene product is 69,000, which is consistent with the calculated molecular weight of 69,378 fro the deduced gene product of the open reading frame. The purified gene product causes the nicking of DNA at the 8th phosphodiester bond 5' and the 5th phosphodiester bond 3' to a thymine dimer when mixed with E. coli UvrA and UvrB proteins and a DNA substrate containing a uniquely located thymine dimer. We conclude that the gene product of the open reading frame is the B. subtilis UvrC protein. Our results suggest that the B. subtilis nucleotide excision repair system is quite similar to that of E. coli. Furthermore, complementation of the UvrA and UvrB proteins from a Gram-negative bacterium with the UvrC protein of Gram-positive B. subtilis indicates a significant evolutionary conservation of the nucleotide excision repair system.
Cleavage of influenza A virus H1 hemagglutinin by swine respiratory bacterial proteases (1997)

Cleavage of influenza A virus hemagglutinin (HA) is required for expression of fusion activity and virus entry into cells. Extracellular proteases are responsible for the proteolytic cleavage activation of avirulent avian and mammalian influenza viruses and contribute to pathogenicity and tissue tropism. The relative contributions of host and microbial proteases to cleavage activation in natural infection remain to be established. We examined 23 respiratory bacterial pathogens and 150 aerobic bacterial isolates cultured from the nasal cavities of pigs for proteolytic activity. No evidence of secreted proteases was found for the bacterial pathogens, including Haemophilus parasuis, Pasteurella multocida, Actinobacillus pleuropneumoniae, Bordetella bronchiseptica, and Streptococcus suis. Proteolytic bacteria were isolated from 7 of 11 swine nasal samples and included Staphylococcus chromogenes, Staphylococcus hyicus, Aeromonas caviae, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Enterococcus sp. Only P. aeruginosa secreted a protease, elastase, that cleaved influenza virus HA. However, compared to trypsin, the site of cleavage by elastase was shifted one amino acid in the carboxy-terminal direction and resulted in inactivation of the virus. Under the conditions of this study, we identified several bacterial isolates from the respiratory tracts of pigs that secrete proteases in vitro. However, none of these proteolytic isolates demonstrated direct cleavage activation of influenza virus HA.
2‐{2‐[3‐(Pyridin‐3‐yloxy)phenyl]‐2H‐tetrazol‐5‐yl}pyridine: A Highly Potent, Orally Active, Metabotropic Glutamate Subtype 5 (mGlu5) Receptor Antagonist. (2005)

Abstract For Abstract see ChemInform Abstract in Full Text.
SIRT7 an emerging sirtuin: deciphering newer roles. (2013)

Sirtuin7 (SIRT7) is a NAD(+)-dependent protein deacetylase which belongs to sirtuin family. Although sirtuin proteins have attracted a great deal of attention, little is known about SIRT7. SIRT7 participates in rDNA transcription in the nucleolus. However, recent reports show that SIRT7 has additional novel functions. This review summarizes all findings about SIRT7, and highlights its critical roles in cellular functions.
Scheduling Single-Armed Cluster Tools With Reentrant Wafer Flows (2006)

A cluster tool for semiconductor manufacturing consists of several single-wafer processing chambers and a wafer-handling robot in a closed environment. The use of cluster tools is extended to reentrant processes such as atomic layer deposition, where a wafer visits a processing chamber more than once. Such a reentrant wafer How complicates scheduling and control of the cluster tool and often causes deadlocks. We examine the scheduling problem for a single-armed cluster tool with various reentrant wafer flows. We develop a convenient method of modeling tool operational behavior with reentrant wafer flows using Petri nets. By examining the net model, we then develop a necessary and sufficient condition for preventing a deadlock. We also show that the cycle time for the asymmetric choice Petri net model for a reentrant wafer How can be easily computed by using the equivalent event graph model. From the results, we systematically develop a mixed integer programming model for determining the optimal tool operation sequence, schedule, and cycle time. We also extend a workload measure for cluster tools with reentrant wafer flows. Finally, we discuss how our results can be used for engineering a cluster tool. We compare two proposed strategies, sharing and dedicating, of operating the parallel processing chambers for identical process steps.
Mild Cognitive Impairment (1999)

Patients who meet the criteria for MCI can be differentiated from healthy control subjects and those with very mild AD. They appear to constitute a clinical entity that can be characterized for treatment interventions.
Efficient insertional mutagenesis in gram-positive bacteria (1995)

Abstracts for oral and poster presentations*INRA, laboratoire de Génétique Microbienne, Jouy-en-Josas (FRA) Diffusion du document : INRA, laboratoire de Génétique Microbienne, Jouy-en-Josas (FRA)
Culpabegreppet – evigt samma ytuttryck, evigt nya konkretiserande avvägningar (2011)

Finns det nagot mer valkant och evigt inom juridiken an det anda sedan romartiden standigt och oforanderligt vidareforda culpauttrycket? Alla kanner ju till det och kan anvanda begreppets ordvandning for att rattsligt forsta och formedla att nagon handlat fel, varit vardslos, oaktsam, slarvig eller … ja, culpos, helt enkelt. Men finns det verkligen ett entydigt innehall i alla dessa utsagor som nyttjar de just namnda glosorna – eller ligger homogeniteten blott i det sprakliga uttrycket for de alltefterhand varierande varderingarna och avvagningarna kring ansvarsproblemet? Givetvis det senare – och det vet egentligen ocksa alla jurister (men ibland kan det vara behagligt att gomma sig bakom det forsatligt otvetydiga ordet). Istallet for att vara en allman handlingsregel eller en moraliserande benamning pa avvikelsen fran det redan fastlagda ratta handlingssattet, handlar culparegeln om en metod for att pa ett specialiserat satt avvaga de komponenter som talar for och emot ansvar i en konkret situation. En ny exemplarisk dom fran Hogsta domstolen demonstrerar detta, och far darfor i denna artikel tjana som utgangspunkt for en analys av komponenterna i detta sa kanda, men anda markligt undflyende, rattsliga begrepp.
Preparation and properties of nido-carborane-specific monoclonal antibodies for potential use in boron neutron capture therapy for cancer. (1995)

As the first step of a research program aimed at developing a bispecific monoclonal antibody system for the delivery of boron-rich molecules to tumor cells for boron neutron capture therapy, monoclonal antibodies (mAbs) were produced against an anionic nido-carborane derivative, 4-[7,8-dicarbadodecahydroundecaborat(-1)-7-yl]butanoic acid. Two IgG subclass mAbs, designated HAW101 and HAW102, were identified that specifically bound the anionic nido-carborane hapten, as well as a variety of other anionic nido-carborane cage derivatives. By using surface plasmon resonance technology, the affinity constants of HAW101 and HAW102 were determined to be 1.9 x 10(9) and 6.8 x 10(8) M-1, respectively. A diverse array of 7-substituted and 7,8-disubstituted anionic nido-carborane derivatives reacted with the mAb HAW101 in competition ELISA, whereas anionic closo-polyhedral boranes showed negligible binding, suggesting a role for the open nido-carborane cage structure. These results suggest that mAbs such as HAW101, which bind anionic nido-carboranes, are useful in the development of bispecific mAbs for specific targeting and enhanced boron delivery to tumor sites.
Interleukins: Biological Properties and Therapeutic Potential (1991)

Abstarct Interleukins are biologically active glycoproteins derived primarily from activated lymphocytes and macrophages. Tremendous insight into the biochemical and biological properties of interleukins has been gained with advances in recombinant DNA technology, protein purification, and cell-culture techniques. The biological properties of interleukins include induction of T-lymphocyte activation and proliferation, augmentation of neutrophil, macrophage, and T-lymphocyte cytotoxicity, and promotion of B lymphocyte and multilineage bone marrow stem-cell precursor growth and differentiation. Interleukins may play a role in the pathogenesis of several important diseases. Interleukin therapy is likely to play an important role in the treatment of cancer, infectious diseases, and immunodeficiency syndromes.
The Adsorption of Sensitizing Dyes in Photographic Emulsions (1952)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Adsorption of Sensitizing Dyes in Photographic EmulsionsW. West, B. H. Carroll, and D. H. WhitcombCite this: J. Phys. Chem. 1952, 56, 9, 1054–1067Publication Date (Print):September 1, 1952Publication History Published online1 May 2002Published inissue 1 September 1952https://pubs.acs.org/doi/10.1021/j150501a007https://doi.org/10.1021/j150501a007research-articleACS PublicationsRequest reuse permissionsArticle Views133Altmetric-Citations41LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
SmartFinger (2013)

In this paper, we demonstrate a method to create a seamless information media 'channel' between the physical and digital worlds. Our prototype, SmartFinger, aims to achieve this goal with a finger-worn camera, which continuously captures images for the extraction of information from our surroundings. With this metaphorical channel, we have created a software architecture which allows users to capture and interact with various entities in our surroundings. The interaction design space of SmartFinger is discussed in terms of smart-connection, smart-sharing and smart-extraction of information. We believe this work will create numerous possibilities for future explorations.
Exhaled breath condensate eicosanoids and sputum eosinophils in asthmatic children: A pilot study (2004)

Cysteinyl leukotrienes (cys-LTs), LTB4 and 8-isoprostane are increased in the exhaled breath condensate (EBC) from asthmatic patients. The aim of this study was to investigate whether the measurement of cys-LTs, LTB4 and 8-isoprostane in EBC can reflect the level of airway inflammation assessed by induced sputum in asthmatic children sensitized to house dust mite (HDM) during natural avoidance of HDM allergens. Twelve children were evaluated at the time of admission (T0) and after 3 months of stay (T1) at the Istituto Pio XII (Misurina, Italian Dolomites 1756 m). Sputum eosinophil percentage and measurement of cys-LTs, LTB4 and 8-isoprostanes in the breath condensate at T0 and T1 were evaluated. Eosinophil percentage in induced sputum was 8.5 +/- 1.1% at T0 and 3.5 +/- 0.4% at T1 (p = 0.011). Neutrophil percentage in sputum was 1.1 +/- 0.5% at T0 and 1.5 +/- 1.0% at T1 (ns). Cys-LTs mean level was 14.24 +/- 4.53 pg/ml at T0 and 4.65 +/- 0.68 pg/ml at T1 (p = 0.0125). LTB4 level was 2.36 +/- 0.19 pg/ml at T0 and 2.41 +/- 0.23 pg/ml at T1 (ns). 8-Isoprostane level reduced from 17.47 +/- 3.18 pg/ml at T0 to 7.36 +/- 3.26 pg/ml at T1 (p = 0.003). This study show that exhaled cys-LTs and 8-isoprostane, as well as eosinophil percentage in induced sputum, are reduced after allergen avoidance in asthmatic children suggesting a potential application of EBC for the non-invasive evaluation of airway inflammation in asthma in allergic asthmatic children.
Safety assessment of nicotinamide riboside, a form of vitamin B3 (2016)

Nicotinamide riboside (NR) is a naturally occurring form of vitamin B<sub>3</sub> present in trace amounts in some foods. Like niacin, it has been shown to be a precursor in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). The safety of Niagen™, a synthetic form of NR, was determined using a bacterial reverse mutagenesis assay (Ames), an in vitro chromosome aberration assay, an in vivo micronucleus assay, and acute, 14-day and 90-day rat toxicology studies. NR was not genotoxic. There was no mortality at an oral dose of 5000 mg/kg. Based on the results of a 14-day study, a 90-day study was performed comparing NR at 300, 1000, and 3000 mg/kg/day to an equimolar dose of nicotinamide at 1260 mg/kg/day as a positive control. Results from the study show that NR had a similar toxicity profile to nicotinamide at the highest dose tested. Target organs of toxicity were liver, kidney, ovaries, and testes. The lowest observed adverse effect level for NR was 1000 mg/kg/day, and the no observed adverse effect level was 300 mg/kg/day.
The growth of a xenograft breast cancer tumor model with engineered hyaluronan-accumulating stroma is dependent on hyaluronan and independent of CD44 (2019)

Hyaluronan accumulation in the tumor microenvironment is associated with poor prognosis in several solid human cancers. To understand the role of stromal hyaluronan in tumor progression, we engineered 3T3HAS3, a hyaluronan-producing fibroblast cell line, by lentiviral transduction of Balb/c 3T3 cells with the <i>human hyaluronan synthase 3</i> <i>(HAS3)</i> gene. 3T3HAS3 cells significantly enhanced tumor growth when co-grafted with MDA-MB-468 cells in nude mice. Immunohistochemical analysis of the xenograft tumors showed that MDA-MB-468 cells were surrounded by hyaluronan-accumulating stroma, closely resembling the morphology observed in human breast cancer specimens. Tumor growth of MDA-MB-468 + 3T3HAS3 co-grafts was greatly reduced upon hyaluronan degradation by lentiviral transduction of a human hyaluronidase gene in 3T3HAS3 cells, or by systemic administration of pegvorhyaluronidase alfa (PEGPH20). In contrast, the growth of the co-graft tumors was not inhibited when CD44 expression was reduced or ablated by small hairpin RNA-mediated CD44 knockdown in MDA-MB-468 cells, CD44 CRISPR knockout in 3T3HAS3 cells, or by grafting these cells in CD44 knockout nude mice. Collectively, these data demonstrate that tumor growth of an engineered xenograft breast cancer model with hyaluronan-accumulating stroma can be dependent on hyaluronan and independent of CD44.
A Papaver somniferum 10-Gene Cluster for Synthesis of the Anticancer Alkaloid Noscapine (2012)

Noscapine is an antitumor alkaloid from opium poppy that binds tubulin, arrests metaphase, and induces apoptosis in dividing human cells. Elucidation of the biosynthetic pathway will enable improvement in the commercial production of noscapine and related bioactive molecules. Transcriptomic analysis revealed the exclusive expression of 10 genes encoding five distinct enzyme classes in a high noscapine-producing poppy variety, HN1. Analysis of an F(2) mapping population indicated that these genes are tightly linked in HN1, and bacterial artificial chromosome sequencing confirmed that they exist as a complex gene cluster for plant alkaloids. Virus-induced gene silencing resulted in accumulation of pathway intermediates, allowing gene function to be linked to noscapine synthesis and a novel biosynthetic pathway to be proposed.
Electrical Remodeling due to Atrial Fibrillation in Chronically Instrumented Conscious Goats (1997)

Electrical remodeling by AF is not mediated by changes in autonomic tone, ischemia, stretch, or ANF. The high rate of electrical activation itself provides the stimulus for the AF-induced changes in AERP.
Steroids versus other immune modulators in the management of allergic dermatoses (2006)

Patients seeking relief from atopic dermatitis have historically had few really effective and safe therapeutic options. Topical calcineurin inhibitors represent an exciting new therapy for atopic dermatitis without the side-effect profile associated with topical corticosteroids. Nonsteroidal formulations incorporating glycyrrhetinic acid/telmesteine/Vitis vinifera extract and palmitoylethanolamide as 'active' ingredients recently entered the market, stressing antipruritic, antiinflammatory, and skin barrier repair. This confabulates against previously designed topical therapy paradigms. These new products may be used as monotherapy or alternatives to steroid agents.
Materials science of DNA (2008)

This article critically reviews the emerging field of DNA materials science, which is expected to add a new horizon to the conventional materials science landscape. The introduction provides a short overview on recent developments in the non-biological application of DNA and DNA science. We discuss the roles of DNA in photonics and optoelectronics, DNA-assisted preparation of inorganic nanoparticles and aggregates, and electrical and magnetic properties of DNA and their influence on nanoscience and technology. We also explore the potential applications of DNA in the development of innovative memory and communication devices. Self-assembly and new sensing technologies based on DNA are only briefly discussed, partly because of the many excellent published reviews on these subjects.
Electrocatalytic Activity and Stability of Nitrogen-Containing Carbon Nanotubes in the Oxygen Reduction Reaction (2009)

Nitrogen-containing carbon nanotubes (NCNTs) were prepared via pyrolysis of acetonitrile over cobalt catalysts at different temperatures to control the nitrogen content. The changes in the chemical and structural properties of undoped CNTs and NCNTs were investigated using high-resolution X-ray photoelectron and Raman spectroscopy. The NCNTs prepared at 550 °C had a higher amount of pyridinic groups and edge plane exposure than the ones prepared at 750 °C. The thermal stability and transformation of these nitrogen functional groups was studied using deconvoluted XP N 1s spectra. The NCNTs show a considerably higher activity in the oxygen reduction reaction in acidic electrolyte compared with undoped CNTs as demonstrated by cyclic voltammetry, rotating disk electrode measurements, and the redox-competition mode of scanning electrochemical microscopy (RC-SECM). Particularly, the NCNT sample prepared at 550 °C exhibited the highest activity, which was about 1 order of magnitude lower than that of a commercial Pt/C sample containing 20 wt % Pt. The oxygen reduction reaction (ORR) performance of this sample showed hardly any signs of deterioration after 3 days, as determined by voltammetric stability tests in H2SO4.