gebhart/patrepeval huggingface dataset:
Insulin and Insulin-like Growth Factor-1 Regulate Tau Phosphorylation in Cultured Human Neurons (1997)

Hyperphosphorylated tau is the major component of paired helical filaments in neurofibrillary lesions associated with Alzheimer's disease. Hyperphosphorylation reduces the affinity of tau for microtubules and is thought to be a critical event in the pathogenesis of this disease. Recently, glycogen-synthase kinase-3 has been shown to phosphorylate tau in vitro and in non-neuronal cells transfected with tau. The activity of glycogen-synthase kinase-3 can be down-regulated in response to insulin or insulin-like growth factor-1 through the activation of the phosphatidylinositol 3-kinase pathway. We therefore hypothesize that insulin or insulin-like growth factor-1 may affect tau phosphorylation through the inhibition of glycogen-synthase kinase-3 in neurons. Using cultured human neuronal NT2N cells, we demonstrate that glycogen-synthase kinase-3 phosphorylates tau and reduces its affinity for microtubules and that insulin and insulin-like growth factor-1 stimulation reduces tau phosphorylation and promotes tau binding to microtubules. We further demonstrate that these effects of insulin and insulin-like growth factor-1 are mediated through the inhibition of glycogen-synthase kinase-3 via the phosphatidylinositol 3-kinase/protein kinase B signaling pathway.
Efficacy, patient‐reported outcomes and safety profile of ATX‐101 (deoxycholic acid), an injectable drug for the reduction of unwanted submental fat: results from a phase III, randomized, placebo‐controlled study (2014)

ATX-101 was effective and well tolerated, and may be an alternative to surgery for patients desiring improvement of their submental profile.
Receptor-Mediated Clearance of Aspergillus Galactomannan (1987)

The fate of radiolabeled Aspergillus fumigatus galactomannan was studied after intravenous injection into rabbits and rats. At 1 hr, the liver contained 35% of the injected dose in rabbits and 30% in rats. Excretion of galactomannan into the urine, measured in rabbits, was another major catabolic route and accounted for 35% of the dose by 24 hr. Immunization of rabbits increased hepatic uptake and decreased urinary excretion. Hepatic uptake in unimmunized rats could be decreased by Saccharomyces cerevisiae mannan, alpha-methyl mannoside, and N-acetylglucosamine, known inhibitors of the macrophage mannose receptor. Autoradiography showed hepatic radiolabeled galactomannan to be concentrated in Kupffer cells, which express the mannosyl receptor for glycoproteins. Macrophage mannosyl receptors may constitute a general mechanism for clearing fungal mannans from the bloodstream.
Trichothecene 3-O-Acetyltransferase Protects Both the Producing Organism and Transformed Yeast from Related Mycotoxins (1998)

Trichothecene mycotoxins such as deoxynivalenol, 4,15-diacetoxyscirpenol, and T-2 toxin, are potent protein synthesis inhibitors for eukaryotic organisms. The 3-O-acetyl derivatives of these toxins were shown to reduce their in vitro activity significantly as assessed by assays using a rabbit reticulocyte translation system. The results suggested that the introduction of an O-acetyl group at the C-3 position in the biosynthetic pathway works as a resistance mechanism for Fusarium species that produce t-type trichothecenes (trichothecenes synthesized via the precursor trichotriol). A gene responsible for the 3-O-acetylation reaction, Tri101, has been successfully cloned from a Fusarium graminearum cDNA library that was designed to be expressed in Schizosaccharomyces pombe. Fission yeast transformants were selected for their ability to grow in the presence of T-2 toxin, and this strategy allowed isolation of 25 resistant clones, all of which contained a cDNA for Tri101. This is the first drug-inactivating O-acetyltransferase gene derived from antibiotic-producing organisms. The open reading frame of Tri101 codes for a polypeptide of 451 amino acid residues, which shows no similarity to any other proteins reported so far. TRI101 from recombinant Escherichia coli catalyzes O-acetylation of the trichothecene ring specifically at the C-3 position in an acetyl-CoA-dependent manner. By using the Tri101 cDNA as a probe, two least overlapping cosmid clones that cover a region of 70 kilobase pairs have been isolated from the genome of F. graminearum. Other trichothecene biosynthetic genes, Tri4, Tri5, and Tri6, were not clustered in the region covered by these cosmid clones. These new cosmid clones are considered to be located in other parts of the large biosynthetic gene cluster and might be useful for the study of trichothecene biosynthesis.
Packet-pair sizing for controlling packet dispersion on wired heterogeneous networks (2013)

The packet-pair structure is a widely used probing technique for measuring link capacity over network paths. In packet-pair probing, the probe-gap value at the link of interest (e.g., the link with the smallest capacity or any link along a path) is determined for link-capacity measurement, which can be affected if dispersion (i.e., separation) of the packet pair is created by the heterogeneous link capacities and cross traffic over an end-to-end path. In this paper, we present an analytical model concerning the effect of heterogeneous link capacities and cross traffic on the packet-pair structure over a multiple-hop path, and define the constraints on the packet sizing for accurate end-link (i.e., the link connected to the destination node of an end-to-end path) capacity measurement. We test the model through numerical evaluations and experimentally, on end-to-end paths with heterogeneous links and different cross traffic loads. The experiments are performed on a testbed and in the Internet.
Collagens of the Chicken Eggshell Membranes (1991)

An immunohistochemical analysis of the eggshell membranes shows the occurrence of type X collagen while type I collagen was not detected by using an appropriate monoclonal antibody with untreated shell membranes. A positive immuno-reaction for type I collagen was obtained after digestion of the shell membranes with pepsin. These observations indicate the possibility that type I collagen epitope was masked by type X collagen and that type X collagen may serve as an inhibitory boundary for biomineralization.
Mass Spectrometric Characterization of the Affinity-Purified Human 26S Proteasome Complex (2007)

The 26S proteasome is a multisubunit complex responsible for degradation of ubiquitinated substrates, which plays a critical role in regulating various biological processes. To fully understand the function and regulation of the proteasome complex, an important step is to elucidate its subunit composition and posttranslational modifications. Toward this goal, a new affinity purification strategy has been developed using a derivative of the HB tag for rapid isolation of the human 26S proteasome complex for subsequent proteomic analysis. The purification of the complex is achieved from stable 293 cell lines expressing a HB-tagged proteasome subunit and by high-affinity streptavidin binding with TEV cleavage elution. The complete composition of the 26S proteasome complex, including recently assigned new subunits, is identified by LC-MS/MS. In addition, all known proteasome activator proteins and components involved in the ubiquitin-proteasome degradation pathway are identified. Aside from the subunit composition, the N-terminal modification and phosphorylation of the proteasome subunits have been characterized. Twelve novel phosphorylation sites from eight subunits have been identified, and N-terminal modifications are determined for 25 subunits, 12 of which have not been previously reported in mammals. We also observe different N-terminal processing of subunit Rpn2, which results in identification of two different N-termini of the protein. This work presents the first comprehensive characterization of the human 26S proteasome complex by affinity purification and tandem mass spectrometry. The detailed proteomic profiling obtained here is significant to future studies aiming at a complete understanding of the structure-function relationship of the human 26S proteasome complex.
Maximizing CNN Accelerator Efficiency Through Resource Partitioning (2017)

Convolutional neural networks (CNNs) are revolutionizing machine learning, but they present significant computational challenges. Recently, many FPGA-based accelerators have been proposed to improve the performance and efficiency of CNNs. Current approaches construct a single processor that computes the CNN layers one at a time; the processor is optimized to maximize the throughput at which the collection of layers is computed. However, this approach leads to inefficient designs because the same processor structure is used to compute CNN layers of radically varying dimensions.
The creation of nanojunctions (2010)

This review describes recent progress in creation of nanojunctions between individual nanoobjects. The accomplishments of various strategies used for nanojunction creation are highlighted and the corresponding challenges are discussed. The possible ongoing development for the creation of device-oriented nanojunctions is speculated upon.
Facial thermography is a sensitive tool to determine antihistaminic activity: comparison of levocetirizine and fexofenadine (2006)

Facial thermography is an objective, non-invasive and sensitive method to study antihistaminic activity at the nose level. Levocetirizine and fexofenadine demonstrate the same activity at 2 h after drug intake, but levocetirizine has a more sustained activity at 24 h.
Report on computational assessment of Tumor Infiltrating Lymphocytes from the International Immuno-Oncology Biomarker Working Group (2020)

Assessment of tumor-infiltrating lymphocytes (TILs) is increasingly recognized as an integral part of the prognostic workflow in triple-negative (TNBC) and HER2-positive breast cancer, as well as many other solid tumors. This recognition has come about thanks to standardized visual reporting guidelines, which helped to reduce inter-reader variability. Now, there are ripe opportunities to employ computational methods that extract spatio-morphologic predictive features, enabling computer-aided diagnostics. We detail the benefits of computational TILs assessment, the readiness of TILs scoring for computational assessment, and outline considerations for overcoming key barriers to clinical translation in this arena. Specifically, we discuss: 1. ensuring computational workflows closely capture visual guidelines and standards; 2. challenges and thoughts standards for assessment of algorithms including training, preanalytical, analytical, and clinical validation; 3. perspectives on how to realize the potential of machine learning models and to overcome the perceptual and practical limits of visual scoring.
Mcl-1 as a therapeutic target in acute myelogenous leukemia (AML) (2013)

The B-cell lymphoma-2 (Bcl-2) family of proteins regulates the intrinsic, or mitochondrial pathway of apoptosis, the final common mechanism of cell death in response to a variety of physiologic and pharmacologic signals, and plays a central role in AML pathogenesis, prognosis and responsiveness to chemotherapy. Traditionally thought to be an important survival factor for multiple myeloma cells, the anti-apoptotic Bcl-2 family protein myeloid cell leukemia-1 (Mcl-1) has recently been shown in preclinical studies to be critical to the development and maintenance of AML, making it an attractive therapeutic target in this disease. Several characteristics, such as its very short half-life, distinguish Mcl-1 from other anti-apoptotic Bcl-2 family members. Additionally, Mcl-1 levels are regulated by a large number of pathways affecting its transcription, translation and degradation. A variety of approaches exploiting these features has been developed to inhibit directly or indirectly the anti-apoptotic function of Mcl-1. Many of these lend themselves well to combination therapies, leading to striking synergism, at least in preclinical models. In this brief review, we highlight some of the more promising strategies targeting Mcl-1 in AML, with a particular emphasis on rational combinations of novel agents.
Prevalence of Diabetic Retinopathy in the United States, 2005-2008 (2010)

In a nationally representative sample of US adults with diabetes aged 40 years and older, the prevalence of diabetic retinopathy and vision-threatening diabetic retinopathy was high, especially among Non-Hispanic black individuals.
3‐Substituted imidazo[1,5‐a]pyridines (1979)

Abstract Cyclization of amides of 2‐aminomethylpyridine gave imidazo[1,5‐ a ]pyridines. In several examples the literature preparation (phosphorus oxychloride) gave extensive tar formation. The use of phosphorus trichloride‐triethylamine (−20°) gave the desired imidazo[1,5‐ a ]pyridines.
Adaptive Processing of Top-k Queries in XML (2005)

The ability to compute top-k matches to XML queries is gaining importance due to the increasing number of large XML repositories. The efficiency of top-k query evaluation relies on using scores to prune irrelevant answers as early as possible in the evaluation process. In this context, evaluating the same query plan for all answers might be too rigid because, at any time in the evaluation, answers have gone through the same number and sequence of operations, which limits the speed at which scores grow. Therefore, adaptive query processing that permits different plans for different partial matches and maximizes the best scores is more appropriate. In this paper, we propose an architecture and adaptive algorithms for efficiently computing top-k matches to XML queries. Our techniques can be used to evaluate both exact and approximate matches where approximation is defined by relaxing XPath axes. In order to compute the scores of query answers, we extend the traditional tf*idf measure to account for document structure. We conduct extensive experiments on a variety of benchmark data and queries, and demonstrate the usefulness of the adaptive approach for computing top-k queries in XML.
Strongly Interacting Plasmon Nanoparticle Pairs:  From Dipole−Dipole Interaction to Conductively Coupled Regime (2004)

We have investigated the optical response of periodic arrays of metallic (gold) nanoparticles composed of a pair of particles on each lattice site. By varying the interparticle separation within the pairs from dielectric proximity to conductive contact on a nanometer scale, we observe an abrupt, large renormalization as well as a splitting of the surface plasmon polariton energy. These spectral anomalies are ascribed to a transition whereupon the interparticle dipole−dipole interaction is shunted and the plasmon polaritons exhibit multipolar behavior, including a very high local concentration of electromagnetic energy in the vicinity of their conductive contact.
Evidence for N-acetoxy-N-2-acetylaminofluorene induced covalent-like binding of some nonhistone proteins to DNA in chromatin (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEvidence for N-acetoxy-N-2-acetylaminofluorene induced covalent-like binding of some nonhistone proteins to DNA in chromatinGerard Metzger and Harold WerbinCite this: Biochemistry 1979, 18, 4, 655–659Publication Date (Print):February 20, 1979Publication History Published online1 May 2002Published inissue 20 February 1979https://pubs.acs.org/doi/10.1021/bi00571a016https://doi.org/10.1021/bi00571a016research-articleACS PublicationsRequest reuse permissionsArticle Views28Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Ultra-low resistive ohmic contacts on n-GaN using Si implantation (1997)

Implanted ohmic contacts were made on molecular beam epitaxy grown GaN materials. Si was implanted at a doping density of about 4×1020 cm-3 to decrease the contact resistance of the contact, followed by an activation anneal at 1150 °C for 30 s. The overlay metal Ti/Au was evaporated. Four-probe measurements were performed on transmission line model patterns. The measured maximum contact resistance was 0.097 Ω mm and the apparent specific contact resistance was 3.6×10−8 Ω cm2.
General LC-MS/MS Method Approach to Quantify Therapeutic Monoclonal Antibodies Using a Common Whole Antibody Internal Standard with Application to Preclinical Studies (2012)

Ligand binding assays (LBAs) are widely used for therapeutic monoclonal antibody (mAb) quantification in biological samples. Major limitations are long method development times, reagent procurement, and matrix effects. LC-MS/MS methods using signature peptides are emerging as an alternative approach, which typically use a stable isotope labeled signature peptide as the internal standard (IS). However, a new IS has to be generated for every candidate, and the IS may not correct for variations at all processing steps. We have developed a general LC-MS/MS method approach employing a uniformly heavy-isotope labeled common whole mAb IS and a common immunocapture for sample processing. The method was streamlined with automation for consistency and throughput. Method qualification of four IgG(2) and four IgG(1) mAbs showed sensitivity of 0.1 μg/mL and linearity of 0.1-15 μg/mL. Quality control (QC) data of these eight mAbs were accurate and precise. The QC performance of the whole molecule labeled IS was better than those of synthetic labeled IS peptides tested. The pharmacokinetic results of two mAbs (an IgG(2) and IgG(1) candidate) dosed in rats were comparable to those of LBA. The general LC-MS/MS method approach overcomes the limitations of current methods to reduce time and resources required for preclinical studies.
Carbohydrate utilization in Streptococcus thermophilus: characterization of the genes for aldose 1-epimerase (mutarotase) and UDPglucose 4-epimerase (1990)

The complete nucleotide sequences of the genes encoding aldose 1-epimerase (mutarotase) (galM) and UDPglucose 4-epimerase (galE) and flanking regions of Streptococcus thermophilus have been determined. Both genes are located immediately upstream of the S. thermophilus lac operon. To facilitate the isolation of galE, a special polymerase chain reaction-based technique was used to amplify the region upstream of galM prior to cloning. The galM protein was homologous to the mutarotase of Acinetobacter calcoaceticus, whereas the galE protein was homologous to UDPglucose 4-epimerase of Escherichia coli and Streptomyces lividans. The amino acid sequences of galM and galE proteins also showed significant similarity with the carboxy-terminal and amino-terminal domains, respectively, of UDPglucose 4-epimerase from Kluyveromyces lactis and Saccharomyces cerevisiae, suggesting that the yeast enzymes contain an additional, yet unidentified (mutarotase) activity. In accordance with the open reading frames of the structural genes, galM and galE were expressed as polypeptides with apparent molecular masses of 39 and 37 kilodaltons, respectively. Significant activities of mutarotase and UDPglucose 4-epimerase were detected in lysates of E. coli cells containing plasmids encoding galM and galE. Expression of galE in E. coli was increased 300-fold when the gene was placed downstream of the tac promoter. The gene order for the gal-lac gene cluster of S. thermophilus is galE-galM-lacS-lacZ. The flanking regions of these genes were searched for consensus promoter sequences and further characterized by primer extension analysis. Analysis of mRNA levels for the gal and lac genes in S. thermophilus showed a strong reduction upon growth in medium containing glucose instead of lactose. The activities of the lac (lactose transport and beta-galactosidase) and gal (UDPglucose 4-epimerase) proteins of lactose- and glucose-grown S. thermophilus cells matched the mRNA levels.
Cys‐scanning mutagenesis: a novel approach to structure—function relationships in polytopic membrane proteins (1998)

The entire lactose permease of Escherichia coli, a polytopic membrane transport protein that catalyzes beta-galactoside/H+ symport, has been subjected to Cys-scanning mutagenesis in order to determine which residues play an obligatory role in the mechanism and to create a library of mutants with a single-Cys residue at each position of the molecule for structure/function studies. Analysis of the mutants has led to the following: 1) only six amino acid side chains play an irreplaceable role in the transport mechanism; 2) positions where the reactivity of the Cys replacement is increased upon ligand binding are identified; 3) positions where the reactivity of the Cys replacement is decreased by ligand binding are identified; 4) helix packing, helix tilt, and ligand-induced conformational changes are determined by using the library of mutants in conjunction with a battery of site-directed techniques; 5) the permease is a highly flexible molecule; and 6) a working model that explains coupling between beta-galactoside and H+ translocation. structure-function relationships in polytopic membrane proteins.
THE PREPARATION OF SOME RARE EARTH FORMATES AND THEIR CRYSTAL STRUCTURES (1962)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTHE PREPARATION OF SOME RARE EARTH FORMATES AND THEIR CRYSTAL STRUCTURESI. Mayer, M. Steinberg, F. Feigenblatt, and A. GlasnerCite this: J. Phys. Chem. 1962, 66, 9, 1737–1738Publication Date (Print):September 1, 1962Publication History Published online1 May 2002Published inissue 1 September 1962https://pubs.acs.org/doi/10.1021/j100815a502https://doi.org/10.1021/j100815a502research-articleACS PublicationsRequest reuse permissionsArticle Views183Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
The Catalytic Dimerization of Oleic Acid in a Continuous Flow Reactor (1972)

Abstract In this investigation a fixed bed reactor, packed with montmorillonite grains, has been used for the semi‐continuous dimerization. The effect of the process parameters, such as temperature, catalyst diameter, water content of the oleic acid and space velocity, on the dimerization yield has been determined. The activity of the montmorillonite decreases slowly in each run. This process may compare favourably to the batch process as used at present in the industry.