gebhart/patrepeval huggingface dataset:
Functional Activity of Substantia Nigra Grafts Reinnervating the Striatum: Neurotransmitter Metabolism and [14C]2‐Deoxy‐d‐glucose Autoradiography (1982)

Dopaminergic innervation of the caudate nucleus in adult rats can be partially restored by the grafting of embryonic substantia nigra into the overlying parietal cortex with concomitant compensation of certain behavioral abnormalities. In this study the function of such grafts was investigated neurochemically by quantification of transmitter metabolism and glucose utilization in the reinnervated target. Rats with unilateral 6-hydroxydopamine lesions of the nigrostriatal bundle received a single graft to the dorsal caudate-putamen and were screened for rotational behavior following 5 mg/kg methamphetamine. The grafts restored dopamine concentrations in the caudate-putamen from initially less than 0.5% to an average of 13.6% of normal in rats with behavioral compensation. The ratio of 3,4-dihydroxyphenylacetic acid to dopamine, which is a measure of the rate of transmitter turnover, were equivalent in transplanted and normal control rats. Moreover, measurements of DOPA accumulation for a 30-min period after DOPA decarboxylase inhibition indicated similar fractional dopamine turnover rates in normal and transplant-reinnervated tissues. Correlations between rotational behavior and dopamine concentrations showed that reinnervation to only 3% of normal was sufficient to counterbalance the motor asymmetry. Measurements of glucose utilization by [14C]deoxyglucose autoradiography indicated equivalent metabolic rates for the grafted tissue and the intact substantia nigra. 6-Hydroxydopamine denervation of the caudate-putamen had no significant effect on neuronal metabolism in that region, nor did subsequent reinnervation from a graft. Grafts, however, were associated with a 16% reduction of glucose uptake in the ipsilateral globus pallidus, indicating a significant transsynaptic influence of the nigral transplants on neuronal metabolism in the host brain. Overall the results indicate that behaviorally functional neuronal grafts spontaneously metabolize dopamine and utilize glucose at rates characteristic of the intact nigrostriatal system. This provides further evidence that ectopic intracortical nigral transplants can reinstate dopaminergic neurotransmission in regions of the host brain initially denervated by the 6-hydroxydopamine lesion.
Vaccination of different strains of mice against cutaneous leishmaniosis: usefulness of membrane antigens encapsulated into liposomes by intraperitoneal and subcutaneous administration. (1997)

The objective of this study was to assess the usefulness of parasite-surface molecules reconstituted into liposomes to vaccinate four different strains of mice (C57BL/10, CBA/ca, C57BL/6 and NZB) with different levels of susceptibility to L. m. mexicana infection and to find out possible increases in specific antibody response after vaccination, but before infection with virulent promastigotes. Mice were vaccinated with parasite membrane antigens incorporated into liposomes and antibody levels were recorded. Vaccination was effective to protect CBA/ca and C57BL/6 but not C57BL/10 mice and NZB animals were naturally resistant. Intraperitoneal (ip) was more effective than the subcutaneous (sc) route of inoculation, and the induction of disease-resistance correlated with the production of IgG anti-Leishmania in CBA/ca, C57BL/6 and C57BL/10 mice.
Dynamic Features of Prothrombin Interaction with Phospholipid Vesicles of Different Size and Composition:  Implications for Protein−Membrane Contact (1996)

The dynamics of prothrombin interaction with membrane vesicles of different size and composition was investigated to ascertain the impact of membrane surface characteristics and particle size on this interaction. Dissociation rates were highly sensitive to membrane composition and varied from about 20/s for membranes of 10% PS to 0.1/s for membranes of 50% PS. Overall affinity also varied by more than two orders of magnitude. Very small differences between prothrombin binding to SUV versus LUV were found. Association with large unilamellar vesicles (LUV of 115 nm diameter) was about 4-fold slower, when expressed on the basis of binding sites, than association with small unilamellar vesicles (SUV, 30 nm diameter) of the same composition. Both reactions proceeded at less than 25% of the collisional limit so that the differences were largely due to intrinsic binding properties. Vesicles of 325 nm diameter showed even slower association velocities. Dissociation rates from LUV were about 2-fold slower than from SUV. Again, these differences arose primarily from intrinsic binding properties. Dissociation conformed to a single first order reaction over a wide range of protein occupancy on the membrane. At very high packing density, the dissociation rate increased by about 2-fold. At equilibrium, prothrombin preferred binding to SUV over LUV by about 2-fold. This very small difference, despite substantial differences in phospholipid headgroup packing and hydrocarbon exposure, appeared inconsistent with an important role for protein insertion into the hydrocarbon region of the membrane. However, prothrombin-membrane interaction may arise from a series of interaction forces that have compensating features at equilibrium. The small differences in prothrombin binding to SUV versus LUV, together with differences in the number of protein binding sites per vesicle, were important to identify mechanisms of substrate delivery to the active site of the prothrombinase enzyme [Lu, Y., & Nelsestuen, G. L. (1996) Biochemistry 35, 8201-8209].
Fibroblast heterogeneity in the healing wound (1995)

Although fibroblasts are traditionally described as static cells providing framework and support for tissues, there is an accumulating body of evidence showing that fibroblasts are a dynamic cell type which exist in functionally and morphologically heterogeneous subpopulations. Fibroblast subsets have been shown to play a critical role in the production and regulation of extracellular matrix components, in wound repair and regeneration, and have been implicated in the pathogenesis of fibrotic conditions. We have reviewed the evidence supporting heterogeneity of fibroblasts from pulmonary, periodontal, and dermal tissues. In addition, we will explore the role fibroblast subpopulations may play in the complex process of wound repair and regeneration.
Development of a Transparent and Ultrahydrophobic Glass Plate (1993)

A transparent glass plate was roughened at the submicron level, and then a chemically adsorbed monolayer of a fluorocarbon compound was formed along the glass surface. The monolayer was prepared using the chemical coupling reaction accompanying dehydrochlorination between a chlorosilyl group of the heptadeca-fluorodecyltrichlorosilane (CF 3 (CF 2 ) 7 (CH 2 ) 2 SiCl 3 : HFTS) surface-active agent and a hydroxyl group on the glass surface. The contact angle of the glass surface relative to water was approximately 155 degrees with transmittance kept at 92%. This glass plate may be useful for various products, such as motor vehicles and building window glass.
Immobilization of antibodies on the self-assembled monolayer by antigen-binding site protection and immobilization kinetic control (2011)

The orientation of the biological molecule immobi-lized on a solid surface has been critical in devel-opment of various applications. In this study, ori-entation of antibody was retained by protecting the antigen-binding site of the antibody prior to immo-bilization to -functionalized mixed self-assembled monolayer (SAM) of 12-mercaptododecanoic acid and 1-heptanethiol. More importantly, the number of immobilization bonds formed between each an-tigen-binding site protected antibody molecule and the solid surface was controlled by optimizing the mole fraction of the activated carboxyl group of the linker molecules in the mixed SAM. The amount of antibody used in this study was approximately equivalent to the amount for one monolayer surface coverage. The resulting activity of protected immo-bilized antibody was about 10 fold higher than that of random immobilized antibody
Textbook of Surgery (1993)

The development of surgery molecular biology in surgery clinical outcomes in surgery homeostasis shock fluid and electrolyte management of the surgical patient principles of preoperative preparation of the surgical patient blood transfusions and disorders of surgical bleeding metabolism in surgical patients surgical aspects of diabetes mellitus anaesthesia wound healing burns principles of operative surgery surgical infections bites and stings trauma surgical complications acute renal failure in surgical patients transplantation immunobiology and immunotherapy of neoplastic disease, melanoma, soft tissue sacromas, tumor markers the breast the thyroid gland the parathyroid glands the pituitary and adrenal gland the oesophagus laparoscopic surgery abdominal wall, umbilicus, peritoneum, mesenteries, omentum and retroperitoneum the acute abdomen the stomach and duodenum the small intestine the colon and rectum the liver the biliary system the pancreas the spleen hernias paediatric surgery surgical disorders of the ears, nose, paranasal sinuses, pharynx and larynx plastic and maxillofacial surgery neurosurgery fractures and dislocations replantation of amputated limbs and digits gynaecolocy the urinary system the male genital system disorders of the lymphatic system venous disorders pulmonary embolism disorders of the arterial system disorders of the lungs, pleura and chest wall the mediastinum surgical disorders of the pericardium the heart.
Principles of hormone replacement therapy in the menopause (2000)

17 beta-estradiol, conjugated equine estrogens, esterified estrogens, and estriol constitute postmenopausal replacement therapy, all of which are in clinical use as oral preparations. Non-oral routes--matrix and reservoir patches, gel--were developed for estradiol, as was the intravaginal administration of estriol and estradiol. Daily doses of 1 mg estradiol(valerate) or 25 micrograms estradiol delivered via a patch or 0.5 mg gel or 0.3 mg conjugated equine estrogens are often sufficient to alleviate climacteric symptoms. Bone resorption may be effectively reduced and bone mineral density maintained by 1 mg estradiol or 25 micrograms transdermal estradiol. Maximal bone sparing dosages are 2 mg estradiol, 50 micrograms transdermal estradiol, 0.625 mg conjugated equine estrogens, and 1.25 mg estrone Estriol, predominantly used for the prevention and or treatment of urogenital symptoms, has no bone sparing effect at the doses in clinical use. Non-oral administration of estradiol may be superior in diabetic women and those with hypertriglyceridemia due to the different metabolism which does not mainly involve the hepatic first pass effect. Epidemiological data do not support any preference of oral versus non-oral routes of administration regarding side-effects such as venous thromboembolism. Progestogens--natural progesterone, derivatives structurally related to progesterone and testosterone, respectively--are necessary for endometrial protection. Sequential use of a progestogen for at least 10 days per month, preferably 12-14 days abolishes the increased incidence of endometrial hyperplasia which is likely to develop with unopposed use of estrogen. Observational studies do not suggest any superiority of a given progestogen regarding cardiovascular risk, prevention of osteoporosis, and cognitive function in postmenopausal women on estrogen replacement therapy. Tibolone, a derivative of norethindrone, is yet another option for replacement therapy. The recommended dose for treatment of climacteric symptoms and prevention of bone loss is 2.5 mg. Controlled clinical studies do not suggest that this compound is superior in achieving amenorrhea compared with continuous combined estrogen progestogen replacement therapy, as available data are inconsistent. In early postmenopause the sequential use of a progestogen in conjunction with an estrogen is the preferred treatment option. With advancing postmenopausal age either continuous combined replacement or tibolone may be choices in case withdrawal bleeding is no longer acceptable for women. However, there are no rigid age limit when to change treatments, the selection of which is largely influenced by the preference of the individual's acceptance of withdrawal bleeding.
In Vivo and In Vitro Specificity of Protein Tyrosine Kinases for Immunoglobulin G Receptor (FcγRII) Phosphorylation (1996)

Human B cells express four immunoglobulin G receptors, FcgammaRIIa, FcgammaRIIb1, FcgammaRIIb2, and FcgammaRIIc. Coligation of either FcgammaRII isoform with the B-cell antigen receptor (BCR) results in the abrogation of B-cell activation, but only the FcgammaRIIa/c and FcgammaIIb1 isoforms become phosphorylated. To identify the FcgammaRII-phosphorylating protein tyrosine kinase (PTK), we used the combination of an in vitro and an in vivo approach. In an in vitro assay using recombinant cytoplasmic tails of the different FcgammaRII isoforms as well as tyrosine exchange mutants, we show that each of the BCR-associated PTKs (Lyn, Blk, Fyn, and Syk) shows different phosphorylation patterns with regard to the different FcgammaR isoforms and point mutants. While each PTK phosphorylated FcgammaRIIa/c, FcgammaRIIb1 was phosphorylated by Lyn and Blk whereas FcgammaRIIb2 became phosphorylated only by Blk. Mutants lacking both tyrosine residues of the immune receptor tyrosine-based activation motif (ITAM) of FcgammaRIIa/c were not phosphorylated by Blk and Fyn, while Lyn-mediated phosphorylation was dependent on the presence of the C-terminal tyrosine of the ITAM. Results obtained in assays using an FcgammaR- B-cell line transfected with wild-type or mutated FcgammaRIIa demonstrated that exchange of the C-terminal tyrosine of the ITAM of FcgammaRIIa/c was sufficient to abolish FcgammaRIIa/c phosphorylation in B cells. Additionally, we could show that Lyn and Fyn bind to FcgammaRIIa/c, with the ITAM being necessary for association. Comparison of the phosphorylation pattern of each PTK observed in vitro with the phosphorylation pattern observed in vivo suggests that Lyn is the most likely candidate for FcgammaRIIa/c and FcgammaRIIb1 phosphorylation in vivo.
Continuous Synthesis of Monodisperse Ag Nanocubes (2017)

The precise control of both the size and shape of Ag nanoparticles strongly influences their optical properties. Although the synthesis of Ag nanocubes with sharp corners and edges has been demonstrated, the ability to scale these approaches with high selectivity remains elusive. In this study, a continuous flow microwave-assisted reactor was used to separate nucleation from growth events, which provides a method to synthesize very uniform single crystalline Ag nanocubes. Nucleation in the microwave zone was enhanced through seed-mediated processes by sulfide formation, and a chemical regulator was used in the growth zone to further improve the sharpness of the edges of the nanocubes. Transmission electron microscopy and optical properties were used to optimize the reaction conditions and Ag nanocubes with edge lengths of 28 and 45 nm were readily synthesized with narrow particle size distributions and high selectivities (>70%). Nanocubes with 28 nm edge lengths were used to prepare films to demonstrate the detection of Rhodamine 6G with concentrations down to 10 nM using surface enhanced Raman spectroscopy. These results indicate that continuous flow approaches have the potential to produce large quantities of uniform Ag nanocubes that can be used for sensing or other applications.
3D light scanning macrography (2001)

The technique of 3D light scanning macrography permits the non‐invasive surface scanning of small specimens at magnifications up to 200×. Obviating both the problem of limited depth of field inherent to conventional close‐up macrophotography and the metallic coating required by scanning electron microscopy, 3D light scanning macrography provides three‐dimensional digital images of intact specimens without the loss of colour, texture and transparency information. This newly developed technique offers a versatile, portable and cost‐efficient method for the non‐invasive digital and photographic documentation of small objects. Computer controlled device operation and digital image acquisition facilitate fast and accurate quantitative morphometric investigations, and the technique offers a broad field of research and educational applications in biological, medical and materials sciences.
Cognitive disorders related to immune dysfunction: Novel animal models for drug development (1986)

Abstract With the recent emphasis on drug development for mental disorders of late life, a need has arisen for animal models useful in preclinical evaluation of potential treatment strategies. Although the animal models now available exhibit behavioral and neurological commonalities with their target clinical syndromes, few models incorporate etiologic similarities. A review of the literature is presented relating immune factors to aging‐associated cognitive disorders, and parallels are drawn to immune factors and related behavioral impairments in autoimmune New Zealand black and nonautoimmune C57BL/6 mice. These mouse strains are proposed as potentially useful immunologic models for both theoretical investigation and drug development.
Teletraffic analysis and mobility modeling of PCS networks (1999)

Channel holding time is of primary importance in teletraffic analysis of PCS networks. This quantity depends on user's mobility which can be characterized by the cell residence time. We show that when the cell residence time is not exponentially distributed, the channel holding time is not exponentially distributed either, a fact also confirmed by available field data. In order to capture the essence of PCS network behaviour, including the characterization of channel holding time, a correct mobility model is therefore necessary. The new model must be good enough to fit field data, while at the same time resulting in a tractable queueing system. We propose a new mobility model, called the hyper-Erlang distribution model, which is consistent with these requirements. Under the new realistic operational assumption of this model, in which the cell residence time is generally distributed, we derive analytical results for the channel holding time distribution, which are readily applicable to the hyper-Erlang distribution models. Using the derived analytical results we demonstrate how the distribution of the cell residence time affects the channel holding time distribution. The results presented in this paper can provide guidelines for field data processing in PCS network design and performance evaluation.
Anatomy of the Auriculotemporal Nerve: Variations in Its Relationship to the Superficial Temporal Artery and Implications for the Treatment of Migraine Headaches (2010)

There are variations in the relationship between the auriculotemporal nerve and the superficial temporal artery. These variations may serve as an anatomical explanation for this point as a source of migraine headaches in some patients. A topographical map of the relationship between these two structures may serve as a guide for surgeons interested in decompressing the nerve from the artery when indicated.
Fetal loss in systemic lupus: association with anticardiolipin antibodies (1985)

SummaryTwenty-three patients with systemic lupus erythematosus who had had one or more spontaneous abortions or intra-uterine deaths were studied retrospectively and compared with a control group. An association was found between maternal anticardiolipin antibodies and fetal loss. This correlation may have therapeutic implications.
Immunodetection of Human Fibrin Using Monoclonal Antibody-64C5 in an Extracorporeal Chicken Model (1985)

The potential use of a fibrin-specific monoclonal antibody-64C5 as a thrombus detecting agent was examined in a quantifiable extracorporeal circulatory model using the chicken. This species was selected because antibody-64C5 did not cross-react with chicken fibrin. Human fibrinogen was clotted with human thrombin and factor XIII on filter paper disks, which were then inserted into an ex-vivo chamber that interrupted an extracorporeal loop between a carotid artery and a jugular vein. Antibody-64C5 was injected intravenously and after an equilibration period, disks bearing human fibrin were placed in the extracorporeal chamber and exposed to circulating antibody. Uptake which was quantified by treating the disks with 125I-goat antimouse-(Fab')2 was rapid, with 70 percent of maximal uptake occurring within 10 min. Fibrinolysis, although measurable, had no adverse effect upon uptake of the monoclonal antifibrin-64C5.
Pea Albumin 1 Subunit b (PA1b), a Promising Bioinsecticide of Plant Origin (2011)

PA1b (Pea Albumin 1, subunit b) is a peptide extract from pea seeds showing significant insecticidal activity against certain insects, such as cereal weevils (genus Sitophilus), the mosquitoes Culex pipiens and Aedes aegyptii, and certain species of aphids. PA1b has great potential for use on an industrial scale and for use in organic farming: it is extracted from a common plant; it is a peptide (and therefore suitable for transgenic applications); it can withstand many steps of extraction and purification without losing its activity; and it is present in a seed regularly consumed by humans and mammals without any known toxicity or allergenicity. The potential of this peptide to limit pest damage has stimulated research concerning its host range, its mechanism of action, its three-dimensional structure, the natural diversity of PA1b and its structure-function relationships.
Colocalization of Inflammatory Response with B7-H1 Expression in Human Melanocytic Lesions Supports an Adaptive Resistance Mechanism of Immune Escape (2012)

Although many human cancers such as melanoma express tumor antigens recognized by T cells, host immune responses often fail to control tumor growth for as yet unexplained reasons. Here, we found a strong association between melanocyte expression of B7-H1 (PD-L1), an immune-inhibitory molecule, and the presence of tumor-infiltrating lymphocytes (TILs) in human melanocytic lesions: 98% of B7-H1(+) tumors were associated with TILs compared with only 28% of B7-H1(-) tumors. Indeed, B7-H1(+) melanocytes were almost always localized immediately adjacent to TILs. B7-H1/TIL colocalization was identified not only in melanomas but also in inflamed benign nevi, indicating that B7-H1 expression may represent a host response to tissue inflammation. Interferon-γ, a primary inducer of B7-H1 expression, was detected at the interface of B7-H1(+) tumors and TILs, whereas none was found in B7-H1(-) tumors. Therefore, TILs may actually trigger their own inhibition by secreting cytokines that drive tumor B7-H1 expression. Consistent with this hypothesis, overall survival of patients with B7-H1(+) metastatic melanoma was significantly prolonged compared with that of patients with B7-H1(-) metastatic melanoma. Therefore, induction of the B7-H1/PD-1 pathway may represent an adaptive immune resistance mechanism exerted by tumor cells in response to endogenous antitumor activity and may explain how melanomas escape immune destruction despite endogenous antitumor immune responses. These observations suggest that therapies that block this pathway may benefit patients with B7-H1(+) tumors.