gebhart/patrepeval huggingface dataset:
THE ROSENMUND-von BRAUN NITRILE SYNTHESIS1 (1941)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTHE ROSENMUND-von BRAUN NITRILE SYNTHESIS1C. F. KOELSCH and A. G. WHITNEYCite this: J. Org. Chem. 1941, 06, 6, 795–803Publication Date (Print):November 1, 1941Publication History Published online1 May 2002Published inissue 1 November 1941https://pubs.acs.org/doi/10.1021/jo01206a002https://doi.org/10.1021/jo01206a002research-articleACS PublicationsRequest reuse permissionsArticle Views2045Altmetric-Citations45LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Immunologic Self-Tolerance Maintained by Cd25+Cd4+Regulatory T Cells Constitutively Expressing Cytotoxic T Lymphocyte–Associated Antigen 4 (2000)

This report shows that cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) plays a key role in T cell-mediated dominant immunologic self-tolerance. In vivo blockade of CTLA-4 for a limited period in normal mice leads to spontaneous development of chronic organ-specific autoimmune diseases, which are immunopathologically similar to human counterparts. In normal naive mice, CTLA-4 is constitutively expressed on CD25(+)CD4(+) T cells, which constitute 5-10% of peripheral CD4(+) T cells. When the CD25(+)CD4(+) T cells are stimulated via the T cell receptor in vitro, they potently suppress antigen-specific and polyclonal activation and proliferation of other T cells, including CTLA-4-deficient T cells, and blockade of CTLA-4 abrogates the suppression. CD28-deficient CD25(+)CD4(+) T cells can also suppress normal T cells, indicating that CD28 is dispensable for activation of the regulatory T cells. Thus, the CD25(+)CD4(+) regulatory T cell population engaged in dominant self-tolerance may require CTLA-4 but not CD28 as a costimulatory molecule for its functional activation. Furthermore, interference with this role of CTLA-4 suffices to elicit autoimmune disease in otherwise normal animals, presumably through affecting CD25(+)CD4(+) T cell-mediated control of self-reactive T cells. This unique function of CTLA-4 could be exploited to potentiate T cell-mediated immunoregulation, and thereby to induce immunologic tolerance or to control autoimmunity.
A new minimally invasive procedure for pudendal nerve stimulation to treat neurogenic bladder: Description of the method and preliminary data (2005)

Chronic pundedal nerve stimulation is feasible. Neurophysiological guidance is mandatory to place the lead near the pudendal nerve either using perineal or posterior approach. Further studies must be carried out to identify the best stimulation parameters and to verify the long term results.
Rolling in P-selectin-deficient mice is reduced but not eliminated in the dorsal skin (1995)

P-selectin-mediated rolling is believed to be important in the recruitment of leukocytes to tissue after ischemia-reperfusion injury. The dorsal skin chamber was used to examine differences in the rolling and stable adhesion of circulating leukocytes in subcutaneous (SC) vessels of P-selectin-deficient and age-matched wild-type mice, both under basal conditions and after ischemia-reperfusion. Rolling in the postcapillary venules in SC tissue of P-selectin-deficient mice was significantly lower than that in wild-type mice under the basal conditions and post-ischemia-reperfusion (P < .05), but was not eliminated by the deletion of the P-selectin gene. No significant difference between P-selectin-deficient and wild-type mice in shear rate or leukocyte-endothelial adhesion was observed up to 24 hours after ischemia-reperfusion. These results show that P-selectin-mediated rolling is not a prerequisite for ischemia-reperfusion-induced leukocyte-endothelial adhesion in the skin.
Large Scale Generation of Functional Megakaryocytes From Human Embryonic Stem Cells (hESCs) Under Stromal-Free Conditions. (2009)

Abstract Abstract 2540 Poster Board II-517 Platelets collected from donors have very limited shelf life and are increasingly needed for transfusions. In contrast to donor dependent cord blood or bone marrow CD34+ stem cells, hESCs are a promising alternative source for continuous in vitro production of platelets under controlled conditions. Current procedures for in vitro generation of megakaryocytes/platelets from hESCs are not efficient and require undefined animal stromal cells. We have developed a novel system to generate megakaryocytes (MKs) from human ES cells under serum and stromal-free conditions. In the current system, hESCs are directed towards megakaryocytes through distinct steps including embryoid body formation and hemangioblast development (Lu et al, Nature Methods, 4:501–509, 2007). A transient bi-potential cell population expressing both CD41a and CD235a markers has been identified at the end of hemangioblast culture. These cells are capable of generating both MKs and erythroid cells as demonstrated by FACS sorting and CFU assays. TPO, SCF and IL-11 are used to further direct MK differentiation of hemangioblasts derived from human ES cells in suspension culture. Currently, up to 2.5×107 MKs (CD41a+) can be generated from 1×106 hESCs, which is approximately 10 times more efficient than recently reported methods (Takayama et al Blood, 111(11):5298–5306, 2008). Without further purification, &gt;90% of live cells from the suspension cultures are CD41a+ and the majority of these cells are also CD42b+ (&gt;70%). These in vitro derived MK cells have morphological characteristics of mature, polyploid MKs as shown by Giemsa staining and immunofluorescent staining of vWF in cytoplasmic granules. Importantly, proplatelet forming cells are constantly observed at the late stage of MK culture indicating that MKs generated in this system are able to undergo terminal differentiation under feeder-free conditions. Platelet-like particles are also detected in culture media by FACS. When plated on OP9 cells, these MKs generate functional platelets that are responsive to thrombin stimulation. In summary, we have established a novel system for the generation of platelet-producing MKs from human ES cells that is suitable for scale up and future preclinical and clinical studies. Disclosures: Li: Stem Cell &amp; Regenerative Medicine International: Employment. Lu:Stem Cell &amp; Regenerative Medicine International: Employment. Feng:Stem Cell &amp; Regenerative Medicine International: Employment. Lanza:Stem Cell &amp; Regenerative Medicine International/Advanced Cell Technology, Inc: Employment.
Metallo-dielectric electromagnetic bandgap structures for suppression and isolation of the parallel-plate noise in high-speed circuits (2003)

A novel approach for the suppression of the parallel-plate waveguide (PPW) noise in high-speed printed circuit boards is presented. In this approach, one of the two conductors forming the PPW is replaced by an electromagnetic bandgap (EBG) surface. The main advantage of the proposed approach over the commonly practiced methods is the omnidirectional noise suppression it provides. For this purpose, two EBG structures are initially designed by utilizing an approximate circuit model. Subsequently, the corresponding band structures are characterized by analytical solutions using the transverse resonance method, as well as full-wave finite-element simulations. The designed EBG surfaces were fabricated and employed in a number of PPW test boards. The corresponding frequency-domain measurements exhibited bandgaps of approximately 2.21 and 3.35 GHz in the frequency range below 6 GHz. More importantly, suppression of the PPW noise by 53% was achieved based on time-domain reflectometry experiments, while maintaining the signal transmission quality within the required specifications for common signaling standards.
PDL oxide enabled pitch doubling (2008)

A double patterning (DP) process is introduced with application for advanced technology nodes. This DP technique is enabled by a novel low-temperature pulsed deposition layer (PDL<sup>TM</sup>) oxide film which is deposited directly on patterned photoresist. In this article, we will report the results of fabrication of sub-50nm features on a 100nm pitch by the PDL-spacer DP process using 0.85 NA dry ArF lithography. This result represents the potential of the PDL-based DP to significantly enhance the resolution of the patterning process beyond the limits of optical lithography. Components of CD variance for this spacer DP scheme will be discussed.
Low cost and precise localization in a remote-powered wireless sensor and identification system (2011)

A low cost and precise localization system based on a remote-powered UWB-RFID tag is presented for wireless identification, sensing, positioning and tracking. Our contribution is to utilize the Impulse Radio Ultra wideband (IR-UWB) communication in a RFID system. Such as conventional RFIDs, a tag captures energy from the received RF signal transmitted by a reader which also carries data and clock. However, instead of backscattering, an Impulse-UWB transmitter is used. By a low power design operation distance of 13.9 meters is achieved. A network consist of several readers provide power and retrieve data from the tags in a wide area. Due to the fine time resolution of the ultra-short pulse in IR-UWB, the UWB receiver in the readers are able to accurately approximate the time of arrival of the signal and based on the time-difference-of-arrival algorithm the position of the tag can be estimated precisely. In the line-of-sight scenario by a two-step acquisition system, ±16.8cm accuracy can be achieved. By a new communication protocol proposed based on slotted-ALOHA anti-collision algorithm, 2000 tags per second can be read. The tag circuitry is designed and implemented in CMOS 180nm technology in a single chip solution.
Expression of Single Chain Antibodies (ScFvs) for c‐myc Oncoprotein in Recombinant Escherichiacoli Membranes by Using the Ice‐Nucleation Protein of Pseudomonassyringae (2000)

The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. In this study, the INP from Pseudomonas syringae was applied as a fusion partner with the single chain antibody fragment (ScFv) against the human oncoprotein c-myc. Two new plasmids pNinaZ-myc and pNinaZScFv-BsaA1 were constructed and cloned into Escherichia coli JM109. The expression of the fusion protein was successfully demonstrated in the cloned cells. The fusion proteins had no effect on the viability of the host cells. Ice nucleation activity measurements and flow cytometry studies were followed to investigate the membrane expression of the fusion protein.
Analysis of the femtosecond isomerization reaction mechanism of rhodopsin (1996)

Summary form only given. Visual excitation is initiated by the absorption of a photon by the 11-cis retinal chromophore bound within the pigment called rhodopsin. The photochemical cis-trans isomerization in rhodopsin is complete in only 200 fs. The extreme speed of this process suggests that the photochemistry involves nonstationary states or vibrational coherence.
Protein expression from exogenous mRNA: Uptake by receptor-mediated endocytosis and trafficking via the lysosomal pathway (2011)

Insertional mutagenesis and the inherent risk of malignancy compromise the clinical use of DNA-based therapies. Being a transient copy of genetic material, mRNA is a safe alternative, overcoming this limitation. As a prerequisite for the development of efficient mRNA-based therapies, we investigated the cellular uptake and intracellular fate of mRNA for the first time. To this end we determined cell-type, dose and energy dependence of mRNA internalisation. Moreover, we employed markers for uptake pathways and cellular compartments to analyse the route of mRNA internalisation and its intracellular destination. Finally, we addressed the involvement of receptors and their nature using a competitor-based approach. We found that all cell types tested were amenable to uptake and expression of naked mRNA. Internalisation mainly occurred via caveolae/lipid raft-rich membrane domains and involved scavenger-receptor(s). Following endocytosis, mRNA eventually accumulated in lysosomes, while part of it escaped into the cytosol giving rise to protein synthesis. Taken together, our findings provide unprecedented insights into the internalisation and trafficking of exogenous mRNA, greatly facilitating the development of effective mRNA-based therapies in the future.
A comparative study of the iron-clearing properties of desferrithiocin analogues with desferrioxamine B in a Cebus monkey model (1993)

A comparative study of the iron-clearing properties of subcutaneously administered desferrioxamine B (DFO) with those of orally administered desferrithiocin sodium salt (1), desmethyl desferrithiocin (2), desazadesmethyl desferrithiocin sodium salt (3), desazadesmethyl desferrithiocin pivaloyloxymethyl ester (4), and desazadesmethyl-5,5- dimethyl desferrithiocin (5) in an iron-loaded Cebus monkey model and a non-iron overloaded bile duct-cannulated rat model is presented. All six drugs, which performed well in rodent studies, demonstrated increased efficiency in the Cebus monkey model. When administered to rodents at a daily dosage of 384 mumol/kg over a period of 10 days, drug 1 demonstrated severe renal toxicity. whereas drugs 3, 4, and 5 exhibited severe gastrointestinal (GI) toxicity. Under the same experimental protocol, drug 2 did not show significant toxic side effects. In addition, to further evaluate the iron-clearing properties of analogue 2, a dose-response study was performed in the primates that showed that iron excretion increased in a dose-dependent fashion.
Biosynthesis of caffeic acid in Escherichia coli using its endogenous hydroxylase complex (2012)

We have successfully established a novel pathway and constructed an E. coli strain for the production of caffeic acid. This work forms a basis for further improvement in production, as well as opens the possibility of microbial synthesis of more complex plant secondary metabolites derived from caffeic acid. In addition, we have identified that TAL is the rate-limiting enzyme in this pathway. Thus, exploration for more active TALs via bio-prospecting and protein engineering approaches is necessary for further improvement of caffeic acid production.
Effect of flax seed ingestion on the menstrual cycle. (1993)

Lignans are a group of phytochemicals shown to have weakly estrogenic and antiestrogenic properties. Two specific lignans, enterodiol and enterolactone, are absorbed after formation in the intestinal tract from plant precursors particularly abundant in fiber-rich food and are excreted in the urine. We evaluated the effect of the ingestion of flax seed powder, known to produce high concentrations of urinary lignans, on the menstrual cycle in 18 normally cycling women, using a balanced randomized cross-over design. Each subject consumed her usual omnivorous, low fiber (control) diet for 3 cycles and her usual diet supplemented with flax seed for another 3 cycles. The second and third flax cycles were compared to the second and third control cycles. Three anovulatory cycles occurred during the 36 control cycles, compared to none during the 36 flax seed cycles. Compared to the ovulatory control cycles, the ovulatory flax cycles were consistently associated with longer luteal phase (LP) lengths (mean +/- SEM, 12.6 +/- 0.4 vs. 11.4 +/- 0.4 days; P = 0.002). There were no significant differences between flax and control cycles for concentrations of either estradiol or estrone during the early follicular phase, midfollicular phase, or LP. Although flax seed ingestion had no significant effect on LP progesterone concentrations, the LP progesterone/estradiol ratios were significantly higher during the flax cycles. Midfollicular phase testosterone concentrations were slightly higher during flax cycles. Flax seed ingestion had no effect on early follicular phase concentrations of DHEA-S, PRL, or sex hormone-binding globulin. Our data suggest a significant specific role for lignans in the relationship between diet and sex steroid action, and possibly between diet and the risk of breast and other hormonally dependent cancers.
Reprints (1986)

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Effects of photochemically immobilized polymer coatings on protein adsorption, cell adhesion, and the foreign body reaction to silicone rubber (1999)

Photochemical immobilization technology was utilized to covalently couple polymers to silicone rubber either at multiple points along a polymer backbone or at the endpoint of an amphiphilic chain. The coating variants then were tested in vitro and in vivo for improvement of desired responses compared to uncoated silicone rubber. All coating variants suppressed the adsorption of fibrinogen and immunoglobulin G, and most also inhibited fibroblast growth by 90-99%. None of the coating variants inhibited monocyte or neutrophil adhesion in vitro. However, the surfaces that supported the highest levels of monocyte adhesion also elicited the lowest secretion of pro-inflammatory cytokines. None of the materials elicited a strong inflammatory response or significantly (p< 0.05) reduced the thickness of the fibrous capsule when implanted subcutaneously in rats. Overall, the most passivating coating variant was an endpoint immobilized polypeptide that reduced protein adsorption, inhibited fibroblast growth by 90%, elicited low cytokine secretion from monocytes, and reduced fibrous encapsulation by 33%. In general, although some coating variants modified the adsorption of proteins and the behavior of leukocytes or fibroblasts in vitro, none abolished the development of a fibrous capsule in vivo.
Performance analysis of thread mappings with a holistic view of the hardware resources (2012)

With the shift to chip multiprocessors, managing shared resources has become a critical issue in realizing their full potential. Previous research has shown that thread mapping is a powerful tool for resource management. However, the difficulty of simultaneously managing multiple hardware resources and the varying nature of the workloads have impeded the efficiency of thread mapping algorithms. To overcome the difficulties of simultaneously managing multiple resources with thread mapping, the interaction between various microarchitectural resources and thread characteristics must be well understood. This paper presents an in-depth analysis of PARSEC benchmarks running under different thread mappings to investigate the interaction of various thread mappings with microarchitectural resources including, L1 I/D-caches, I/D TLBs, L2 caches, hardware prefetchers, off-chip memory interconnects, branch predictors, memory disambiguation units and the cores. For each resource, the analysis provides guidelines for how to improve its utilization when mapping threads with different characteristics. We also analyze how the relative importance of the resources varies depending on the workloads. Our experiments show that when only memory resources are considered, thread mapping improves an application's performance by as much as 14% over the default Linux scheduler. In contrast, when both memory and processor resources are considered the mapping algorithm achieves performance improvements by as much as 28%. Additionally, we demonstrate that thread mapping should consider L2 caches, prefetchers and off-chip memory interconnects as one resource, and we present a new metric called L2-misses-memory-latency-product (L2MP) for evaluating their aggregated performance impact.
Modeling of Interaction of Hydraulic Fractures in Complex Fracture Networks (2012)

Abstract A recently developed unconventional fracture model (UFM) is able to simulate complex fracture network propagation in a formation with pre-existing natural fractures. Multiple fracture branches can propagate simultaneously and intersect/cross each other. Each open fracture exerts additional stresses on the surrounding rock and adjacent fractures, which is often referred to as "stress shadow" effect. The stress shadow can cause significant restriction of fracture width, leading to greater risk of proppant screenout. It can also alter the fracture propagation path and drastically affect fracture network patterns. It is hence critical to properly model the fracture interaction in a complex fracture model. A method for computing the stress shadow in a complex hydraulic fracture network is presented. The method is based on an enhanced 2D Displacement Discontinuity Method with correction for finite fracture height. The computed stress field is compared to 3D numerical simulation in a few simple examples and shows the method provides a good approximation for the 3D fracture problem. This stress shadow calculation is incorporated in the UFM. The results for simple cases of two fractures shows the fractures can either attract or repel each other depending on their initial relative positions and compares favorably with an independent 2D non-planar hydraulic fracture model. Additional examples of both planar and complex fractures propagating from multiple perforation clusters are presented, showing that fracture interaction controls the fracture dimension and propagation pattern. In a formation with small stress anisotropy, fracture interaction can lead to dramatic divergence of the fractures as they tend to repel each other. However, even when stress anisotropy is large and fracture turning due to fracture interaction is limited, stress shadowing still has a strong effect on fracture width, which affects the injection rate distribution into multiple perforation clusters, and hence overall fracture network geometry and proppant placement.
Epicutaneous exposure of protein antigen induces a predominant Th2-like response with high IgE production in mice (1996)

Hapten-induced contact hypersensitivity has been well-characterized in humans as well as in animal models. However, it is not clear whether or not protein Ag can directly sensitize epicutaneously and induce a primary immune response. We demonstrated in this study, for the first time, that through epicutaneous exposure protein Ag in the absence of adjuvant sensitizes animals and induces a predominant Th2-like response. Furthermore, mice receiving repeated protein Ag sustained elevated levels of specific IgE. This animal model can be used to investigate the molecular mechanism controlling the differential Th1/Th2 development in skin diseases.