gebhart/patrepeval huggingface dataset:
The effect of supplements of formaldehyde-treated casein on the partitioning of nutrients between cow and calf in lactating Bos indicus × Bos taurus heifers fed a roughage diet (1988)

The effect of a supplement of formaldehyde-treated casein on partition of nutrients and productivity of Bos indicus x Bos taurus (50% x 50%) first-calf heifers was studied. Sixteen pregnant heifers were fed a long-chopped roughage diet (13 g N/kg organic matter) ad lib. for approximately 3 weeks before calving and for 16 weeks after calving. Half the animals were supplemented with formaldehyde-treated casein (300 g per day). There were also 8 non-pregnant, non-lactating controls, half of which were supplemented with formaldehyde-treated casein. Supplementation had no significant effect on intake, irrespective of the physiological state of the heifers. There was a significant (P &amp;lt; 0.05) positive multiple regression between peak intake during lactation and the weight change of both heifer and calf in the first 8 weeks of lactation. Milk yield was not significantly affected by supplementation for the first 8 weeks. In the second half of lactation supplemented heifers produced significantly (P &amp;lt; 0.05) less milk, but not less milk solids, than unsupplemented heifers. During the latter half of lactation there was a highly significant (P &amp;lt; 0.001) positive relationship between milk yield and lactose secretion. The mean (¦ s.e.m.) length of the period of anoestrus after calving was 17 ¦ 2.5 and 12 ¦ 1.7 weeks for unsupplemented and supplemented heifers respectively. The length of the period of anoestrus was positively related to milk production (P &amp;lt; 0.01) and peak intake during lactation (P &amp;lt; 0.05). Supplemented lactating heifers had significantly (P &amp;lt; 0.05) higher plasma insulin concentrations and significantly (P &amp;lt; 0.01) lower plasma growth hormone concentrations than unsupplemented lactating heifers. In supplemented heifers in the second 8 weeks of lactation plasma insulin concentration was significantly (P &amp;lt; 0.01) negatively correlated with milk yield and yield of milk constituents. Supplementation with formaldehyde-treated casein did not increase roughage intake but did affect the partition of nutrients via a positive insulin and negative growth hormone response. Nutrients were preferentially diverted into maternal body growth rather than milk synthesis in the second half of lactation.
TMC114, a Novel Human Immunodeficiency Virus Type 1 Protease Inhibitor Active against Protease Inhibitor-Resistant Viruses, Including a Broad Range of Clinical Isolates (2005)

The purpose of this study was to characterize the antiviral activity, cytotoxicity, and mechanism of action of TMC114, a novel human immunodeficiency virus type 1 (HIV-1) protease inhibitor (PI). TMC114 exhibited potent anti-HIV activity with a 50% effective concentration (EC50) of 1 to 5 nM and a 90% effective concentration of 2.7 to 13 nM. TMC114 exhibited no cytotoxicity at concentrations up to 100 muM (selectivity index, >20,000). All viruses in a panel of 19 recombinant clinical isolates carrying multiple protease mutations and demonstrating resistance to an average of five other PIs, were susceptible to TMC114, defined as a fold change in EC50 of <4. TMC114 was also effective against the majority of 1,501 PI-resistant recombinant viruses derived from recent clinical samples, with EC50s of <10 nM for 75% of the samples. In sequential passage experiments using HIV-1 LAI, two mutations (R41T and K70E) were selected. One selected virus showed a 10-fold reduction in susceptibility to TMC114, but <10-fold reductions in susceptibility to the current PIs (atazanavir was not assessed), except saquinavir. However, when the selected mutations were introduced into a laboratory strain by site-directed mutagenesis, they had no effect on susceptibility to TMC114 or other PIs. There was no evidence of antagonism between TMC114 and any currently available PIs or reverse transcriptase inhibitors. Combinations with ritonavir, nelfinavir, and amprenavir showed some evidence of synergy. These results suggest that TMC114 is a potential candidate for the treatment of both naive and PI-experienced patients with HIV.
Relationships between gene trees and species trees. (1988)

It is well known that a phylogenetic tree (gene tree) constructed from DNA sequences for a genetic locus does not necessarily agree with the tree that represents the actual evolutionary pathway of the species involved (species tree). One of the important factors that cause this difference is genetic polymorphism in the ancestral species. Under the assumption of neutral mutations, this problem can be studied by evaluating the probability (P) that a gene tree has the same topology as that of the species tree. When one gene (allele) is used from each of the species involved, the probability can be expressed as a simple function of Ti = ti/(2N), where ti is the evolutionary time measured in generations for the ith internodal branch of the species tree and N is the effective population size. When any of the Ti's is less than 1, the probability P becomes considerably less than 1.0. This probability cannot be substantially increased by increasing the number of alleles sampled from a locus. To increase the probability, one has to use DNA sequences from many different loci that have evolved independently of each other.
Bacteriological evidence of antibiotic failure in pneumococcal lower respiratory tract infections (2002)

The global pandemic of antimicrobial resistance, particularly in the pneumococcus, has had a major impact on the management of community-acquired pneumonia. A number of prospective and retrospective studies have analysed the impact of penicillin resistance on clinical outcome in pneumonia. Pharmacodynamic principles predicting success when the antibiotic dose exceeds the minimum inhibitory concentration (MIC) for 40-50% of the dosing interval have proved remarkably accurate. There is no evidence of bacteriological failure of penicillins active against resistant strains. There is a single report of the failure of the less active agent, ticarcillin. High dose oral and intravenous amoxicillin should treat strains with MICs < or = 4 microg x mL(-1), as should high doses of intravenous penicillin, ceftriaxone and cefotaxime. Strains of pneumococci resistant to these agents at an MIC > or = 8 microg x mL(-1) are rare at the present time. Most other cephalosporins are less active and should not be used empirically for drug-resistant Streptococcus pneumoniae. Bacteriological failures of cefazolin, cefuroxime and ceftazidime have been reported. There is increasing evidence of bacteriologically confirmed macrolide failure of pneumonia therapy at MICs > or = 4 microg x mL(-1). The molecular basis of the resistance is irrelevant if the MIC is in that range or higher. Double mutants in the parC and gyrA genes lead to fluoroquinolone resistance that has been found to cause bacteriological failure of the fluoroquinolones, particularly levofloxacin and ciprofloxacin, in the management of pneumonia and exacerbations of chronic bronchitis. Two mutations in these genes can greatly increase the MICs of all the marketed fluoroquinolones, and raise the prospect of failure of therapy even with the more active ones. However, demonstration of bacteriological failure of gatifloxacin or moxifloxicin has not yet been reported. High dose, active beta-lactams or fluoroquinolones with enhanced activity against Gram positive pathogens remain the drugs of choice for the management of community-acquired pneumonia caused by the drug-resistant pneumococcus.
Inhibition of p70S6K2 down-regulates Hedgehog/GLI pathway in non-small cell lung cancer cell lines (2009)

We report herein that inhibition of p70S6K2, known as a downstream effector of the PI3K pathway, remarkably decreases GLI-mediated transactivation in NSCLC by reducing phosphorylated-GSK3beta followed by GLI1 degradation. These results infer that p70S6K2 is a potential therapeutic target for NSCLC with hyperactivated HH/GLI pathway.
Relationship of alveolar permeability and lung inflammation in patients with active diffuse infiltrative lung disease detected by 99Tcm-DTPA radioaerosol inhalation lung scintigraphy and quantitative 67Ga lung scans (1994)

The alveolar permeability (AP) in 21 patients with active diffuse infiltrative lung disease (ILD) was measured by 99Tcm-diethylenetriaminapentaacetate (DTPA) radioaerosol inhalation lung scintigraphy. The degree of AP damage in ILD was presented as the slope (% min-1) of the time-activity curve from the dynamic lung image. Meanwhile, the quantitative Ga lung scan (GA) was performed as a Ga uptake index (GUI) to evaluate the severity of lung inflammation in active ILD. The results show that no good correlation between the degree of AP damage and lung inflammation was found. When the patients were divided into two groups of (A) eight active ILD with normal chest X-ray (X-ray) and (B) 13 active ILD with abnormal X-ray, there was no significant difference between groups A and B for the DTPA and Ga results. In conclusion, the relationship between the degree of AP damage and lung inflammation in active ILD is not significant. This was not consistent with X-ray findings. There was, however, a significant difference between those with ILD and normal controls.
La matière organique en contexte archéologique : problématiques et approches (2006)

National audience
Thermal Denaturation:  A Useful Technique in Peptide Mass Mapping (2000)

The use of thermal denaturation of proteins prior to in-solution digestion and mass spectral peptide mass mapping is reported. Thermal denaturation is preferred over chemical denaturation because it does not require purification/concentration prior to mass spectral analysis. Enzymatic digestions of proteins that are resistant to proteolysis are significantly enhanced by thermal denaturation. Native proteins that are sensitive to proteolysis show similar or slightly lower digestion yields following thermal denaturation. Proteins that are resistant to digestion become more susceptible to digestion, independent of protein size, following thermal denaturation. For example, amino acid sequence coverage from digest fragments increases from 15 to 86% in myoglobin and from 0 to 43% in ovalbumin. This leads to more rapid and reliable protein identification by MALDI peptide mass mapping. Although some proteins aggregate upon thermal denaturation, the protein aggregates are easily digested by trypsin and generate sufficient numbers of digest fragments for protein identification.
Developing an institutional framework for rewarding excellence in teaching: a case study (1998)

Outlines an institutional framework for identifying and rewarding excellence in teaching, drawing on an initiative developed at the University of Lincolnshire and Humberside. Describes the strategies adopted by the excellence in teaching working group, the outcomes of the project, and identifies how these have been implemented in the university’s promotions policy. Case study offers a framework for practitioners involved in raising the quality and profile of teaching in higher education through a recognition of the achievement of excellence.
Dual targeting of the proteasome regulates survival and homing in Waldenström macroglobulinemia (2008)

Waldenström macroglobulinemia (WM) is an incurable low-grade B-cell lymphoma characterized by high protein turnover. We dissected the biologic role of the proteasome in WM using 2 proteasome inhibitors, NPI-0052 and bortezomib. We found that NPI-0052 inhibited proliferation and induced apoptosis in WM cells, and that the combination of NPI-0052 and bortezomib induced synergistic cytotoxicity in WM cells, leading to inhibition of nuclear translocation of p65NF-kappaB and synergistic induction of caspases-3, -8, and -9 and PARP cleavage. These 2 agents inhibited the canonical and noncanonical NF-kappaB pathways and acted synergistically through their differential effect on Akt activity and on chymotrypsin-like, caspaselike, and trypsinlike activities of the proteasome. We demonstrated that NPI-0052-induced cytotoxicity was completely abrogated in an Akt knockdown cell line, indicating that its major activity is mediated through the Akt pathway. Moreover, we demonstrated that NPI-0052 and bortezomib inhibited migration and adhesion in vitro and homing of WM cells in vivo, and overcame resistance induced by mesenchymal cells or by the addition of interleukin-6 in a coculture in vitro system. Theses studies enhance our understanding of the biologic role of the proteasome pathway in WM, and provide the preclinical basis for clinical trials of combinations of proteasome inhibitors in WM.
CD68 expression is markedly different in Crohnʼs disease and the colitis associated with chronic granulomatous disease (2009)

This phenomenon provides physicians with a simple and valuable tool to identify CGD in patients with colonic inflammation.
The Theory and Design of Fractal Antenna Arrays (2009)

One of the most fruitful areas of fractal electrodynamics research to date concerns the application of fractal geometry to antenna engineering. In this chapter we provide a comprehensive overview of recent developments in the field of fractal antenna engineering, with particular emphasis placed on the theory and design of fractal arrays. Several important properties of fractal arrays will be presented and discussed in this chapter. These include the frequency-independent multiband characteristics of fractal arrays, schemes for realizing low sidelobe designs, systematic approaches to thinning, and the ability to develop rapid beamforming algorithms by exploiting the recursive nature of fractals.
Hemophagocytic lymphohistiocytosis: pathogenesis and treatment (2013)

Hemophagocytic lymphohistiocytosis (HLH) is not an independent disease but rather a life-threatening clinical syndrome that occurs in many underlying conditions and in all age groups. HLH is the consequence of a severe, uncontrolled hyperinflammatory reaction that in most cases is triggered by an infectious agent. Persistent stimulation of lymphocytes and histiocytes results in hypercytokinemia, leading to the characteristic symptoms of HLH. Genetic defects in familial HLH and in immunodeficiency syndromes associated with albinism affect the transport, processing, and function of cytotoxic granules in natural killer cells and cytotoxic T lymphocytes. This leads to defective killing of target cells and a failure to contract the immune response. The defects are increasingly found also in adolescents and adults. Acquired HLH occurs in autoinflammatory and autoimmune diseases (macrophage activation syndrome) and in patients with iatrogenic immunosuppression or with malignancies, but also in otherwise healthy persons with infections. Treatment of HLH aims at suppressing hypercytokinemia and eliminating the activated and infected cells. In genetic HLH, hematopoietic stem cell transplantation (HSCT) is needed for the correction of the immune defect. Treatment modalities include immunosuppressive, immunomodulatory, and cytostatic drugs; T-cell antibodies; and anticytokine agents. Using immunochemotherapy, familial HLH, which had been invariably fatal, has become a curable disease with more than 50% survivors. Reduced intensity conditioning for HSCT, which is associated with less transplantation-related mortality, will further improve cure rates.
The rat albumin promoter: cooperation with upstream elements is required when binding of APF/HNF1 to the proximal element is partially impaired by mutation or bacterial methylation. (1989)

We have characterized in the accompanying paper (P. Herbomel, A. Rollier, F. Tronche, M.-O. Ott, M. Yaniv, and M. C. Weiss, Mol. Cell. Biol. 9:4750-4758, 1989) six different elements in the albumin promoter. One of them, the proximal element (PE), is the binding site for a strictly liver specific factor, APF/HNF1. This binding site contains a bacterial DAM DNA methylase methylation target sequence which, when methylated, decreases the affinity of the protein for this element. When the different albumin promoter constructions were prepared in an Escherichia coli deoxyadenosine methylase-negative strain, the respective contributions of the elements to the overall promoter activity were strikingly different. An intact proximal element plus the TATA box gave almost full transcriptional activity in transient transfection experiments and only in differentiated hepatoma cells of line H4II, whereas the distal elements (distal element III [DEIII], the NF1-binding site DEII, and the E/CBP-binding site DEI) had become essentially dispensable. Mutations affecting the CCAAT box showed only a two- to threefold decrease. When PE was methylated, mutated, or replaced by the homologous element from the alpha-fetoprotein gene, activity in the context of the short promoter (PE plus the TATA box) was abolished. However, activity was restored in the presence of the upstream elements, showing that cooperation with factors binding to the CCAAT box and distal elements favors the functional interaction of the liver-specific APF/HNF1 factor with lower-affinity binding sites.
Effect of low doses of interleukin-2 injected perilymphatically and peritumorally in patients with advanced primary head and neck squamous cell carcinoma. (1989)

For 10 days, four patients with advanced squamous cell carcinoma of the head and neck received two daily injections of 100 units of natural IL-2, one around the tumor, the second near the tumor-draining lymph nodes. No side effects were observed. Histologic and ultrastructural examination of tumor and lymph nodes recovered at surgery showed that in many instances neoplastic cells were intermingled with numerous lymphocytes and eosinophils. Lymph nodes displayed hyperplasia of both cortical and paracortical areas and epithelioid venules infiltrated by lymphocytes and granulocytes. In a few cases, a decrease or disappearance of neoplastic lesions was also documented both clinically and histologically.
Use of Plastic Commercial Off-the-Shelf (COTS) Microcircuits for Space Applications (2003)

Commercial-off-the-shelf (COTS) plastic encapsulated microcircuits (PEM) are candidate-packaging technologies for spacecraft due to their enhanced performance, lower weight and lower cost. Much of the electronics used in space applications would be considered obsolete by everyday standards. This is primarily due the cost and time required for full space qualification. In order to gain the performance available in today’s electronics, COTS PEMs offer a viable path. PEMs can weigh half as much as their counter part ceramic packages. A lighter package results in a smaller overall payload for the same board functionality, a concern of critical importance for space missions because the payload mass dictates the launch vehicle requirements. Costs can be potentially reduced by using screening, accelerated testing or partial qualification techniques to complement the existing commercial qualification as well as by the reduced package materials costs. Assessing the risk associated with potentially lower reliability devices, engineers within the commercial and aerospace industries are using trade-off and risk analysis to aid in reducing spacecraft system cost while increasing performance and maintaining high reliability. In this paper we will outline the issues facing the use of COTS PEMs for spaceflight hardware from the aspect of both the electronic active devices as well as their packages. Finally, we will provide some guidelines for their use.
“Saying One Thing and Doing Another”: Examining the Impact of Event Order on Hypocrisy Judgments of Others (2005)

This study investigated whether the temporal order of people's expressed statements and their behaviors affected others' judgments of hypocrisy, and why. It was proposed that hypocrisy would be greater when a statement establishing a personal standard preceded a behavior violating that standard as opposed to the reverse order. This order effect occurred in three studies, generalizing across two topic areas (healthy living and safe sex) and for both normative and non-normative statements (pro/anti-safe sex). Mediation analyses indicated that the reverse order mitigated against hypocrisy because the target's inconsistency was attributed to dispositional change. The discussion addresses additional variables likely to affect hypocrisy and the relationship of this research to hypocrisy paradigms in dissonance.
Molecular basis of ocular abnormalities associated with proximal renal tubular acidosis (2001)

Proximal renal tubular acidosis associated with ocular abnormalities such as band keratopathy, glaucoma, and cataracts is caused by mutations in the Na(+)-HCO(3)(-) cotransporter (NBC-1). However, the mechanism by which NBC-1 inactivation leads to such ocular abnormalities remains to be elucidated. By immunological analysis of human and rat eyes, we demonstrate that both kidney type (kNBC-1) and pancreatic type (pNBC-1) transporters are present in the corneal endothelium, trabecular meshwork, ciliary epithelium, and lens epithelium. In the human lens epithelial (HLE) cells, RT-PCR detected mRNAs of both kNBC-1 and pNBC-1. Although a Na(+)-HCO(3)-cotransport activity has not been detected in mammalian lens epithelia, cell pH (pH(i)) measurements revealed the presence of Cl(-)-independent, electrogenic Na(+)-HCO(3)-cotransport activity in HLE cells. In addition, up to 80% of amiloride-insensitive pH(i) recovery from acid load in the presence of HCO(3)(-)/CO(2) was inhibited by adenovirus-mediated transfer of a specific hammerhead ribozyme against NBC-1, consistent with a major role of NBC-1 in overall HCO(3)-transport by the lens epithelium. These results indicate that the normal transport activity of NBC-1 is indispensable not only for the maintenance of corneal and lenticular transparency but also for the regulation of aqueous humor outflow.
An antisense oligonucleotide against SOD1 delivered intrathecally for patients with SOD1 familial amyotrophic lateral sclerosis: a phase 1, randomised, first-in-man study (2013)

The ALS Association, Muscular Dystrophy Association, Isis Pharmaceuticals.
Gene Mapping of Rotavirus Double-Stranded RNA Segments by Northern Blot Hybridization: Application to Segments 7, 8, and 9 (1983)

Cloned DNA copies of double-stranded RNA segments 7, 8, and 9 of UK bovine rotavirus were nick-translated with [alpha-(32)P]ATP and hybridized to double-stranded RNA of various rotavirus strains which had been separated on long polyacrylamide gels and then transferred to o-aminophenylthioether paper. Specific hybridization of the UK calf clones to the separated RNA segments allowed the corresponding genes of four different rotaviruses to be rapidly determined.
A Picture Browsing System (1981)

We describe a system for storing pictures in a database and retrieving them from a remote location over a low bit-rate channel. Even with compression techniques, it takes a long time to transmit a picture, making search for a picture of desired attributes time-consuming. Our system improves the human interaction with the picture database by constructing an auxiliary text database containing a list of attributes of each picture, a hierarchical encoder-decoder, and a light pen to select the areas of picture buildup. Picture searching (or browsing) takes place in two stages: in the first stage, knowing the required picture attributes, a user selects a subset of pictures by matching attributes to the text database; in the second stage, these selected pictures are displayed hierarchically so that a low resolution picture is reproduced first and made sharper gradually. A light pen allows the user to give priority to upgrade selected areas or reject a picture. Our techniques of hierarchical coding are simple to implement. Informal tests indicate that it is much easier to browse through a picture database using this system and that the time to retrieve a picture of given attributes decreases considerably compared to sequential picture presentation.