gebhart/patrepeval huggingface dataset:
Registering multiview range data to create 3D computer objects (1995)

Concerns the problem of range image registration for the purpose of building surface models of 3D objects. The registration task involves finding the translation and rotation parameters which properly align overlapping views of the object so as to reconstruct from these partial surfaces, an integrated surface representation of the object. The registration task is expressed as an optimization problem. We define a function which measures the quality of the alignment between the partial surfaces contained in two range images as produced by a set of motion parameters. This function computes a sum of Euclidean distances from control points on one surfaces to corresponding points on the other. The strength of this approach is in the method used to determine point correspondences. It reverses the rangefinder calibration process, resulting in equations which can be used to directly compute the location of a point in a range image corresponding to an arbitrary point in 3D space. A stochastic optimization technique, very fast simulated reannealing (VFSR), is used to minimize the cost function. Dual-view registration experiments yielded excellent results in very reasonable time. A multiview registration experiment took a long time. A complete surface model was then constructed from the integration of multiple partial views. The effectiveness with which registration of range images can be accomplished makes this method attractive for many practical applications where surface models of 3D objects must be constructed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
An Introduction to Helium Ion Microscopy (2006)

Extended abstract of a paper presented at Microscopy and Microanalysis 2006 in Chicago, Illinois, USA, July 30 – August 3, 2005
Programming with transactional coherence and consistency (TCC) (2004)

Transactional Coherence and Consistency (TCC) offers a way to simplify parallel programming by executing all code within transactions. In TCC systems, transactions serve as the fundamental unit of parallel work, communication and coherence. As each transaction completes, it writes all of its newly produced state to shared memory atomically, while restarting other processors that have speculatively read stale data. With this mechanism, a TCC-based system automatically handles data synchronization correctly, without programmer intervention. To gain the benefits of TCC, programs must be decomposed into transactions. We describe two basic programming language constructs for decomposing programs into transactions, a loop conversion syntax and a general transaction-forking mechanism. With these constructs, writing correct parallel programs requires only small, incremental changes to correct sequential programs. The performance of these programs may then easily be optimized, based on feedback from real program execution, using a few simple techniques.
Pulsed-spray thrombolysis of arterial and bypass graft occlusions. (1991)

Pulsed-spray thrombolysis is accomplished through forceful injection of a spray of highly concentrated urokinase into clot by using catheters with multiple side holes. We previously reported the immediate technical efficacy of the method in eight arterial and 10 bypass graft occlusions. We now describe the clinical efficacy of the method in a second, larger series of 23 native artery occlusions and 25 bypass graft occlusions. Transluminal angioplasty was performed after thrombolysis in 21 of the arteries and 24 of the bypass grafts. Initial thrombolysis was observed in all artery occlusions and all but one bypass graft occlusion with an average time for pulsed-spray lysis of 65 +/- 28 min in native arteries and 93 +/- 38 min in bypass grafts. Recanalization with improvement in symptoms or distal pulses after thrombolysis and angioplasty was achieved in 74% of treated arterial occlusions and 92% of treated graft occlusions. Of the 15 arteries that were recanalized and did not require adjunctive surgery, seven remained patent at 3-28 months follow-up. Nine of 23 recanalized bypass grafts required early adjunctive surgery. Of the nine synthetic and five saphenous vein grafts successfully recanalized and not requiring surgical revision, the mean patency was 4.3 +/- 3.1 months and 3.0 +/- 2.2 months, respectively. Minor complications were seen in 23% of cases. The two major complications (4%) involved one groin hematoma requiring surgery and one episode of gastrointestinal hemorrhage. We conclude that combined pulsed-spray thrombolysis and angioplasty achieve rapid and consistent arterial and graft recanalization with minimal risk. The method offers a favorable alternative to standard thrombolytic therapy of arterial occlusions. In occluded synthetic and vein bypass grafts, the technique is sometimes beneficial, either alone or combined with surgical revision.
Compact multibeam imaging antenna for automotive radars (2003)

A compact multi-beam 77 GHz antenna has been developed for automotive applications. The antenna consists of a hemispherical Teflon lens with backside metallization, which is fed by endfire tapered slot antennas (TSAs) on a 127 /spl mu/m-thick Duroid/sup TM/ substrate. The TSAs are arranged in a circular arc covering 40/spl deg/ around the hemispherical lens. The measured patterns result in E and H-plane patterns with a 3 dB beamwidth of 5.5/spl deg/, a sidelobe level of -18 to -20 dB and a cross-polarization level of -19 dB. The calculated efficiency of this antenna system is 54%. The off-axis antenna patterns are identical to the broadside patterns due to the symmetrical structure of the antenna. The antenna can provide coverage using a circular feed array around the hemispherical Teflon lens.
The neurofibromatosis 2 (NF2) tumor suppressor gene encodes multiple alternatively spliced transcripts (1994)

Neurofibromatosis type 2 (NF2) is an autosomal dominantly-inherited disorder predisposing affected individuals to tumors of multiple cell types in the central nervous system, including meningiomas. A candidate tumor suppressor gene for this disorder has recently been cloned; the protein product of this gene has a predicted role in linking integral membrane proteins with the cytoskeleton. Utilizing reverse transcription-polymerase chain reaction (RT-PCR) analyses, we have identified a number of alternatively spliced transcription products encoded by the NF2 gene. These alternative splice variants were detected in RNA isolated from several sources, including primary leptomeningeal tissue and an established line of leptomeningeal cells (LMC). Several of these variants delete previously identified coding regions of this gene. Moreover, two of these splice variants add previously unrecognized exons to the NF2 coding region. These identified splice forms will serve as natural reagents for the functional dissection of the NF2 protein product(s). They also should be considered in studies investigating mutations of this gene in members of NF2 families and in tumor analyses.
Spastic paraplegia, epilepsy, and mental retardation in several members of a family: A novel genetic disorder (1993)

We report on a family in which an association between spastic paraplegia and epilepsy has been observed. This disorder is an autosomal dominant trait with incomplete penetrance and variable expressivity. The onset was limited to the first four decades of life; the symptoms were typically those of progressive weakness and spasticity of lower limbs. Epilepsy was present in members of three of the four generations on whom we have information. The concomitance of spastic paraplegia and epilepsy in several members of the same family is unlikely to be fortuitous and probably represents the pleiotropic effect of a single mutant gene.
System verification of the JMEX mission residual motion requirements with integrated modeling (2005)

The Jupiter Magnetospheric Explorer (JMEX) is a UV observatory operating in an earth orbit proposed as part of NASA's Small Explorer (SMEX) class of missions. To meet mission requirements the residual jitter portion of the imaging error budget is set at 0.079 arcsec (3&#963;) over a 33.3 ms frame integration time and 0.01 arcsec (3&#963;) for all frequency content higher than 15 Hz. These requirements are challenging for a small, low cost mission and require some innovative system solutions to achieve these goals. The solution, discussed in the paper, was to combine several jitter rejection techniques fine-balanced reaction wheel mounted on an isolation assembly, post processing using science images and reaction wheel momentum control. This paper focuses primarily on meeting the high frequency portion of the requirements. To facilitate system performance verification, we leveraged an integrated model toolset, EOSyM (End-to-end Optical System Model), developed and used on various other advanced space-based missions over the last 9 years. Starting with individual subsystem models for the reaction wheel disturbances, the coupled payload/ spacecraft structural dynamics model, and the optical design, we were able to evaluate the end-to-end LOS performance under varying reaction wheel speeds. At the end we found that the requirements could be met by maintaining the reaction wheels operating range within a well-defined speed band. This paper describes the mission, the technical challenges, the integrated model, and system performance results.
Regression to the mean: what it is and how to deal with it (2004)

RTM is a ubiquitous phenomenon in repeated data and should always be considered as a possible cause of an observed change. Its effect can be alleviated through better study design and use of suitable statistical methods.
Pulse Transit Time in the Analysis of Autonomic Nervous System Effects on the Cardiovascular System (1980)

ABSTRACT The purpose of this study was the development of a simple method for assessing autonomic nervous system (ANS) effects on the cardiovascular system. Pulse transit time (PTT) is the interval between ventricular electrical activity and the peripheral appearance of the pulse (here measured by ear densitography). Six normal subjects were studied with six drugs which either enhanced or blocked activity of one of the three major divisions of the ANS: parasympathetic, beta‐sympathetic, alpha‐sympathetic. Data analysis revealed characteristic patterns of change in PTT and heart rate (HR) which permitted differentiation of five of the six drugs' effects. Vagal enhancement (edrophonium) and alpha‐sympathetic enhancement (phenylephrine)—which reflexly also increases vagal tone to the heart—could not be distinguished from one another unless blood pressure also was monitored. Monitoring of PTT and HR offers a simple, non‐invasive method which can be used on‐line to evaluate ANS effects on the cardiovascular system.
High-Speed in Situ X-ray Scattering of Carbon Nanotube Film Nucleation and Self-Organization (2012)

The production of high-performance carbon nanotube (CNT) materials demands understanding of the growth behavior of individual CNTs as well as collective effects among CNTs. We demonstrate the first use of grazing incidence small-angle X-ray scattering to monitor in real time the synthesis of CNT films by chemical vapor deposition. We use a custom-built cold-wall reactor along with a high-speed pixel array detector resulting in a time resolution of 10 msec. Quantitative models applied to time-resolved X-ray scattering patterns reveal that the Fe catalyst film first rapidly dewets into well-defined hemispherical particles during heating in a reducing atmosphere, and then the particles coarsen slowly upon continued annealing. After introduction of the carbon source, the initial CNT diameter distribution closely matches that of the catalyst particles. However, significant changes in CNT diameter can occur quickly during the subsequent CNT self-organization process. Correlation of time-resolved orientation data to X-ray scattering intensity and height kinetics suggests that the rate of self-organization is driven by both the CNT growth rate and density, and vertical CNT growth begins abruptly when CNT alignment reaches a critical threshold. The dynamics of CNT size evolution and self-organization vary according to the catalyst annealing conditions and substrate temperature. Knowledge of these intrinsically rapid processes is vital to improve control of CNT structure and to enable efficient manufacturing of high-density arrays of long, straight CNTs.
Cartilage tissue engineering with novel nonwoven structured biomaterial based on hyaluronic acid benzyl ester (1998)

The aim of this study was to investigate the possibility of using the benzyl ester of hyaluronic acid (HYAFF 11), a recently developed semisynthetic resorbable material, as a scaffold for the culture of human nasoseptal chondrocytes in tissue-engineering procedures of cartilage reconstruction. Different techniques such as immunohistochemistry, scanning electron microscopy, and confocal laser scanning microscopy were used to study the behavior, morphology, and phenotype expression of the chondrocytes, which were initially expanded and then seeded on the material. The nonwoven cell carrier allowed good viability and adhesivity of the cells without any surface treatment with additional substances. Furthermore, the cultured cells expressed cartilage-specific collagen type II, indicating that they were able to redifferentiate within the scaffold of HYAFF 11 and were able to retain a chondrocyte phenotype even after a long period of in vitro conditions. Nevertheless, the expression of collagen type I, which was produced by dedifferentiated or incompletely redifferentiated chondrocytes, was noticeable. Additional data were obtained by subcutaneous implantation of samples seeded with human cells in the in vivo model of the athymic nude mouse. The results after 1 month revealed the development of tissue similar to hyaline cartilage. This study is promising for the use of this scaffold for tissue engineering of cartilage replacements.
Understanding Web Services: XML, WSDL, SOAP, and UDDI (2002)

(NOTE: Each chapter concludes with a Summary.) Preface. Acknowledgments. Introduction. 1. Introducing Web Services. The Basics of Web Services. A Simple Example: Searching for Information. The Next Generation of the Web. Interacting with Web Services. RPC-Oriented Interactions. Document-Oriented Interactions. The Technology of Web Services. Usage Example. XML: The Foundation. WSDL: Describing Web Services. SOAP: Accessing Web Services. UDDI: Publishing and Discovering Web Services. XML for Business Collaboration: ebXML. Web Services versus Other Technologies. Additional Technologies. Vendor Approaches to Web Services. 2. Describing Information: XML. A Simple Example. Instance and Schema. Data Type and Programming Language. More on XML Schemas and DTDs. Processing XML Documents. Namespaces. Transformation. XSLT. XPath. Document Structure. Mapping Tools. A Simple Example (Revisited). XML Specifications and Information. XML Specifications Related to Web Services. General Information. 3. Describing Web Services: WSDL. WSDL Basics. WSDL Elements. The Extensible WSDL Framework. Defining Message Data Types. Defining Operations on Messages. Mapping Messages to Protocols. Putting It All Together. Importing WSDL Elements. WSDL-Related Namespaces. Extensions for Binding to SOAP. 4. Accessing Web Services: SOAP. A Simple Example. The SOAP Specification. SOAP Envelope. SOAP Header. SOAP Body. SOAP Faults. RPC Convention. Data Type Mapping. HTTP Binding. Version Control. SOAP Message Processing. SOAP Use of Namespaces. Changes in the v1.2 Draft. SOAP Multipart MIME Attachments. SOAP in the Context of Existing Systems. SOAP's Future Directions. 5. Finding Web Services: UDDI Registry. The UDDI Organization. The Concepts Underlying UDDI. How UDDI Works. UDDI Data Model. Generic Data. The Business Entity. The Binding Template. The tModel. UDDI SOAP APIs. Inquiry APIs. Publisher APIs. Usage Scenario. Updating the Registry. Retrieving Information. Using WSDL with UDDI. UDDI for Private Use. UDDI Support for SOAP, Complex Business Relationships, and Unicode. SOAP. Unicode. 6. An Alternative Approach: ebXML. Overview of ebXML. A Simple Example. Deploying ebXML. The ebXML Specifications. Architectural Overview. 7. Web Services Architecture: Additional Technologies. Security. WS-License and WS-Security. Process Flow. XLANG. Transaction Coordination. BTP. Extended Transactions. Messaging. WS-Inspection. WS-Referral. WS-Routing. BEEP. Reliable HTTP. Web Services Foundations. RosettaNet. XML-RPC. 8. Implementing Web Services. Implementation Architectures. The Major Implementation Streams. Microsoft's .NET. J2EE and Application Servers. Application Server Vendor View. Java APIs for Web Services. J2EE Initiatives for Additional Technologies. Understanding .NET versus J2EE. Vendor Views on Adoption of Web Services Technologies. The Questionnaire. BEA Systems. Cape Clear. Hewlett-Packard. IBM. IONA. Microsoft. Oracle. Sun Microsystems. Systinet. Others. Implementations of ebXML. Index. 0201750813T05082002
Anticoagulant protein C pathway defective in majority of thrombophilic patients [see comments] (1993)

A defect involving poor anticoagulant response to activated protein C (APC), an anticoagulant serine protease known to inactivate factors Va and VIIIa in plasma, was recently reported and the existence of a novel APC cofactor was suggested. To define the frequency of this defect among 25 venous thrombophilic patients with no identifiable laboratory test abnormality and among 22 patients previously identified with heterozygous protein C or protein S deficiency, the APC-induced prolongation of the activated partial thromboplastin time assay for these patients was compared with results for 35 normal subjects. The results show that this new defect in anticoagulant response to APC is surprisingly present in 52% to 64% of the 25 patients, ie, in the majority of previously undiagnosed thrombophilia cases, but is not present in 20 of 22 heterozygous protein C or protein S deficient patients, suggesting that the new factor is a risk factor independent of protein C or protein S deficiency. The results demonstrate that abnormalities in the anticoagulant protein C pathway are present in the majority of thrombophilic patients.
Autologous platelets as a source of proteins for healing and tissue regeneration (2003)

Platelets are known for their role in haemostasis where they help prevent blood loss at sites of vascular injury. To do this, they adhere, aggregate and form a procoagulant surface leading to thrombin generation and fibrin formation. Platelets also release substances that promote tissue repair and influence the reactivity of vascular and other blood cells in angiogenesis and inflammation. They contain storage pools of growth factors including PDGF, TGF-beta?and VEGF as well as cytokines including proteins such as PF4 and CD40L. Chemokines and newly synthesised active metabolites are also released. The fact that platelets secrete growth factors and active metabolites means that their applied use can have a positive influence in clinical situations requiring rapid healing and tissue regeneration. Their administration in fibrin clot or fibrin glue provides an adhesive support that can confine secretion to a chosen site. Additionally,the presentation of growth factors attached to platelets and/or fibrin may result in enhanced activity over recombinant proteins. Dental implant surgery with guided bone regeneration is one situation where an autologous platelet-rich clot clearly accelerates ossification after tooth extraction and/or around titanium implants. The end result is both marked reductions in the time required for implant stabilisation and an improved success rate. Orthopaedic surgery, muscle and/or tendon repair, reversal of skin ulcers, hole repair in eye surgery and cosmetic surgery are other situations where autologous plate-lets accelerate healing. Our aim is to review these advances and discuss the ways in which platelets may provide such unexpected beneficial therapeutic effects.
N-hydroxysulfosuccinimide active esters: bis(N-hydroxysulfosuccinimide) esters of two dicarboxylic acids are hydrophilic, membrane-impermeant, protein cross-linkers (1982)

We have synthesized and characterized N-hydroxysulfosuccinimide, a new hydrophilic ligand for the preparation of active esters. We have incorporated this ligand into two new protein cross-linking reagents, 3,3'-dithiobis(sulfosuccinimidyl propionate) and bis(sulfosuccinimidyl) suberate. In experiments with rabbit muscle aldolase, it is demonstrated that both of these reagents are highly efficient protein cross-linkers at physiological pH and that 3,3'-dithiobis(sulfosuccinimidyl propionate) is quantitatively cleavable by reduction under mild conditions. In experiments with intact human erythrocytes and erythrocyte membranes, it is shown that both reagents are membrane impermeant and that when erythrocytes are treated with either reagent, both cross-link subunits of the anion channel (band 3) to covalent dimers at the extracytoplasmic membrane face.
Nanosecond pulse Generator using fast recovery diodes for cell electromanipulation (2005)

Design and operation of a fast recovery diode based pulse generator is presented. The generator produces 3.5-ns-wide, 1200 V amplitude unipolar pulses or +/-600-V bipolar pulses into 50-/spl Omega/ load at the maximum repetition rate of 100 kHz. Pulses shorter than 10 ns are essential for the studies of biological cell response to high electric fields while avoiding ordinary electroporation effects dominant at long pulses. Bipolar pulses are used for the studies of biological cell response to high electric fields when the net transfer of charge is undesirable. The bipolar pulse is produced from a unipolar pulse with the help of a shorted transmission line. This transmission line delays and inverts the initial pulse, so the output is the sum of the initial and the inverted and delayed pulses. The use of mass-produced fast recovery surface-mount rectifier diodes in this circuit substantially simplifies the generator and results in low cost and very small footprint. Similar diode switched pulse generators have been described in the literature using mostly custom fabricated snap-recovery diodes. Here we give an example of an ordinary low-cost diode performing similarly to the custom fabricated counterpart. The diode switched circuit relaxes the requirement on the speed of the main closing switch; in our case, a low-cost power metal-oxide semiconductor field-effect transistor (MOSFET)-saturable core transformer combination.
Cloning, expression, purification, and characterization of the acid -mannosidase from Trypanosoma cruzi (1998)

The acid alpha-mannosidase of Trypanosoma cruzi is a broad-specificity hydrolase involved in the catabolism of glycoconjugates, presumably in the digestive vacuole. We have cloned the alpha-mannosidase gene from a T.cruzi epimastigote genomic library. The alpha-mannosidase gene was determined to be single copy by Southern analysis, and similar sequences were not detected in genomic digests of either Trypanosoma brucei or Leishmania donovani. The coding region was subcloned into the Pichia pastoris expression vector pPICZ, and alpha-mannosidase activity was detected in the medium of induced cultures. The recombinant alpha-mannosidase demonstrated a pH optimum, inhibition by swainsonine, Km, and substrate specificity consistent with the characteristics of the alpha-mannosidase previously purified from T.cruzi epimastigotes. The recombinant enzyme was purified 103-fold from the culture medium of Pichia pastoris and had a native molecular mass of 359 kDa by gel filtration. A combination of SDS-PAGE, deglycosylation with endo H, and NH2-terminal sequencing indicates that the enzyme is originally synthesized as a homodimeric polypeptide that is subsequently cleaved to form a heterotetramer composed of 57 and 46 kDa subunits. A polyclonal antibody raised to the recombinant enzyme was shown to immunoprecipitate the alpha-mannosidase from T.cruzi cell extracts and will be used in future immunolocalization studies.