gebhart/patrepeval huggingface dataset:
PRMT8, a New Membrane-bound Tissue-specific Member of the Protein Arginine Methyltransferase Family (2005)

Protein arginine methylation is a common post-translational modification that has been implicated in signal transduction, RNA processing, transcriptional regulation, and DNA repair. A search of the human genome for additional members of the protein arginine N-methyltransferase (PRMT) family of enzymes has identified a gene on chromosome 12 that we have termed PRMT8. This novel enzyme is most closely related to PRMT1, although it has a distinctive N-terminal region. The unique N-terminal end harbors a myristoylation motif, and we have shown here that PRMT8 is indeed modified by the attachment of a myristate to the glycine residue after the initiator methionine. The myristoylation of PRMT8 results in its association with the plasma membrane. The second singular property of PRMT8 is its tissue-specific expression pattern; it is largely expressed in the brain. A glutathione S-transferase fusion protein of PRMT8 has type I PRMT activity, catalyzing the formation of omega-NG-monomethylated and asymmetrically omega-NG,NG-dimethylated arginine residues on a recombinant glycine- and arginine-rich substrate. PRMT8 is thus an active arginine methyltransferase that is membrane-associated and tissue-specific, two firsts for this family of enzymes.
A Stereospecific Route to 6-Substituted 7-Azabicyclo [3.2.1] oct-2-enes. (1983)

Abstract N-Carbethoxy-3-exo- or 3-endo-substituted-2-azabicyclo-[2.2.2]oct-5-enes 3 provide the title compounds 1 by a stereospeci-fic route involving bromination, elimination, and reductive dehalo-genation.
Dynamic channel assignment in cellular radio (2002)

Dynamic channel assignment algorithms for cellular systems are developed. The algorithms are compared with an easily simulated bound. Using this bound, it is demonstrated that in the case of homogeneous spatial traffic distribution, some of these algorithms are virtually unbeatable by any channel assignment algorithm. These algorithms are shown to be feasible for implementation in current cellular systems. For the examples considered, in the interesting range of blocking probabilities (2-4%), the dynamic channel assignment algorithms yielded an increase of 60-80% in the carried traffic over the best-known fixed channel assignment.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Protective effect of human granulocyte colony-stimulating factor on microbial infection in neutropenic mice (1987)

A purified human granulocyte colony-stimulating factor (hG-CSF) was studied for its protective effect on the induction of neutropenia and enhanced susceptibility to microbial infections in mice receiving cyclophosphamide (CPA). A severe reduction in peripheral blood neutrophils was induced 4 days after injection with 200 mg of CPA per kg although the level normalized rapidly thereafter. When mice were injected subcutaneously once a day with 2.5 micrograms of hG-CSF beginning on the day after CPA injection, the reduction was prevented markedly, even 4 days later. On the other hand, in mice receiving CPA 4 days prior to infection, a weakened resistance to intraperitoneal challenge with a strain of Pseudomonas aeruginosa was induced. This weakened resistance was dose-dependently restored to normal by four daily injections with hG-CSF. A daily dose of 1.0 microgram was required for complete restoration, although hG-CSF did not directly inhibit bacterial growth in vitro. In hG-CSF-treated mice, morphologically mature neutrophils migrated rapidly into the peritoneal cavities where bacteria were inoculated, followed by a rapid elimination of bacteria from the locality as compared with controls. In addition, the same treatment with hG-CSF was able to protect significantly against systemic infections caused by Serratia marcescens, Escherichia coli, Staphylococcus aureus, and Candida albicans. These data show the possibility that prophylactic therapy with hG-CSF may augment the resistance of immunocompromised patients to infections.
Comparative material issues for fast reliable switching in STT-RAMs (2011)

With its fast write and read, small cell size, non-volatility and excellent endurance, Spin Transfer Torque-RAM (STT-RAM) has a high potential of dominating the embedded and standalone memory world in the near future. In this paper, the suitability of different classes of magnetic materials constituting the STT-RAM free layer is reviewed for faster switching and thermal stability. We identify the following material classes for faster switching in the thermally stable free-layer of a STT-RAM: (a) In-plane materials with high H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">K</sub> and low M <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">S</sub> . While the high H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">K</sub> deters the magnetization during the easy to hard axis switching, it helps with switching past the equator, making the switching speeds for high and low H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">K</sub> materials comparable. However, high H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">K</sub> materials benefit from higher thermal stability. (b) Perpendicular materials with low damping have the same switching speed as in-plane materials but greater switching probability because of a lower critical current. The demagnetization field helps the free layer to start switching to the hard axis, but hinders it from switching further to the easy axis beyond the equator. (c) Anti-ferromagnetically capped partially-perpendicular materials. Capping with a Va layer decreases the demagnetization field, which promotes faster switching.
Correlation of Cyclooxygenase-2 Expression With Molecular Markers, Pathological Features and Clinical Outcome of Transitional Cell Carcinoma of the Bladder (2003)

COX-2 is commonly expressed in patients with bladder TCC. Using the cutoff of 10% abnormal COX-2 expression is associated with the degree of invasiveness, alterations in TGF-beta1 and pRB/p16 pathways, and loss of cell adhesion. While COX-2 expression has limited prognostic value in patients with bladder TCC, it may serve as a target for therapy with selective COX-2 inhibitors.
Denaturation of circular or linear DNA facilitates targeted integrative transformation of Streptomyces coelicolor A3(2): possible relevance to other organisms (1997)

Using Streptomyces coelicolor A3(2) protoplasts, the number of transformants obtained by homologous recombination of incoming double-stranded circular DNA with the recipient chromosome was greatly stimulated by simple denaturation of the donor DNA. This procedure was very effective with inserts over a ca. 100-fold size range, the largest tested being ca. 40-kb inserts in cosmids. These observations led to transformation experiments with linearized cloned DNA and randomly sheared genomic DNA. In both cases, DNA denaturation led to significant levels of transformation. Most of the transformants had resulted from the predicted homologous recombination events. A number of genetic manipulations will be made easier or possible by these procedures.
Assertion: Prefetching With GET Is Not Good (2001)

In this study, we develop and apply a methodology to reduce an existing monitoring network to find an optimal configuration of a smaller network. We use a criterion based on locally weighted regression with two different weight functions. The methodology is applied to the Dutch national SO2 network and offers the possibility to include different politically relevant options in the model by weight criteria. Because full enumeration of all monitoring networks is impossible, a combinatorial search algorithm is applied to find a (sub)optimal solution. If different (optimal) monitoring networks result from different criteria, then the best can be selected.
StarGAN-VC: Non-parallel many-to-many voice conversion with star generative adversarial networks (2018)

This paper proposes a method that allows non-parallel many-to-many voice conversion (VC) by using a variant of a generative adversarial network (GAN) called StarGAN. Our method, which we call StarGAN-VC, is noteworthy in that it (1) requires no parallel utterances, transcriptions, or time alignment procedures for speech generator training, (2) simultaneously learns many-to-many mappings across different attribute domains using a single generator network, (3) is able to generate converted speech signals quickly enough to allow real-time implementations and (4) requires only several minutes of training examples to generate reasonably realistic-sounding speech. Subjective evaluation experiments on a non-parallel many-to-many speaker identity conversion task revealed that the proposed method obtained higher sound quality and speaker similarity than a state-of-the-art method based on variational autoencoding GANs.
Fiber optic chemical sensors using a modified conducting polymer cladding (2001)

A new class ofsensors has been designed and prepared based on replacing the original cladding material on a small section of an optical fiber with a conducting polymer or other environmentally sensitive material. Vapor induced chemical interactions with the polymer result in refractive index and optical absorption changes in the polymer cladding. These changes lead to an optical intensity modulation induced within the multi-mode optical fiber. Polyaniline and polypyrrole were used as the modified cladding material on the fiber core. An in-situ deposition method was used to produce uniform thin film coatings of the electronic polymer on the optical fiber. It was found that optimization ofthe sensor sensitivity can be achieved by selecting the proper incident wavelength, excitation conditions, and optical detection technique. Chemical sensors were developed and tested for detection ofHCl and NH3 vapors along with the reducing agent hydrazine. The results clearly demonstrate that conjugated polymer coated fiber optics represent a promising new approach for the detection of volatile toxic gasses.
Compact, multi-exposure speckle contrast optical spectroscopy (SCOS) device for measuring deep tissue blood flow (2017)

Speckle contrast optical spectroscopy (SCOS) measures absolute blood flow in deep tissue, by taking advantage of multi-distance (previously reported in the literature) or multi-exposure (reported here) approach. This method promises to use inexpensive detectors to obtain good signal-to-noise ratio, but it has not yet been implemented in a suitable manner for a mass production. Here we present a new, compact, low power consumption, 32 by 2 single photon avalanche diode (SPAD) array that has no readout noise, low dead time and has high sensitivity in low light conditions, such as <i>in vivo</i> measurements. To demonstrate the capability to measure blood flow in deep tissue, healthy volunteers were measured, showing no significant differences from the diffuse correlation spectroscopy. In the future, this array can be miniaturized to a low-cost, robust, battery operated wireless device paving the way for measuring blood flow in a wide-range of applications from sport injury recovery and training to, on-field concussion detection to wearables.
Effect of PS-PVD production throughput on Si nanoparticles for negative electrode of lithium ion batteries (2018)

Silicon nanoparticles (Si-NPs) have been produced by plasma spray physical vapor deposition at throughput as high as 1 kg h−1 (17 g min−1) and the effect on the battery performance is investigated. When the Si powder feed-rate is changed from 1 to 17 g min−1, although the average primary particle size increases to 50 nm, the cycle capacity of the batteries using these Si-NPs is improved slightly owing to their less agglomerated structure. In contrast, when Ni is added to Si feedstock, the cycle capacity is improved at 1 g min−1 due to modified Si-NP structure having SiNi2 interface. Whereas, the batteries with the Si-NP produced at 17 g min−1 shows significant decrease in the cycle capacity because of the excess Ni silicide formation that is resulted from the elevated co-condensation point and the increased reaction area at high throughputs despite the constant Ni concentration in the feedstock.
Interleukin 2 (IL2) PE40 is cytotoxic to cells displaying either the p55 or p70 subunit of the IL2 receptor. (1988)

IL2-PE40 is a chimeric protein composed of human interleukin 2 (IL2) genetically fused to the amino terminus of a modified form of pseudomonas exotoxin (PE). Internalization of IL2 via the individual p55 and p70 subunits of the IL2 receptor was studied using IL2-PE40 on several mouse and human cell lines expressing either the p55, the p70, or both IL2 receptor subunits. Internalization was assessed by measuring inhibition of protein synthesis caused by the toxin moiety of IL2-PE40. The results demonstrate that IL2 internalization is mediated by either the p55 receptor subunit or by the p70 subunit but is much more efficient when high affinity receptors composed of both subunits are present. IL2-PE40 is a powerful reagent for studying IL2 receptor interactions and for analyzing pathways of the immune response and its regulation.
Quantitative Metabolome Analysis Using Capillary Electrophoresis Mass Spectrometry (2003)

A new approach for the comprehensive and quantitative analysis of charged metabolites by capillary electrophoresis mass spectrometry (CE-MS) is proposed. Metabolites are first separated by CE based on charge and size and then selectively detected using MS by monitoring over a large range of m/z values. This method enabled the determination of 352 metabolic standards and its utility was demonstrated in the analysis of 1692 metabolites from Bacillus subtilis extracts, revealing significant changes in metabolites during B. subtilis sporulation.
Acoustical Pattern Recognition of Knee Joint Diseases (1972)

An improved sound retrieval system and more accurate analysis technique were developed to extract distinguishing acoustical signatures of sound emitted by normal and pathological knee joints during active movements. Background noise was minimized using the principle of “noise cancellation” with a double microphone-differential amplifier setup. Two identical sensitive condenser microphones and an FM recorder with flat responses in the audio range were used. The knee noise was found to be transient in nature and recordings were made on magnetic tape “loops” for “real time” analysis. Waveform, spectral, and statistical (correlation and probability) analyses were performed. Preliminary studies of 30 knee recordings covering normal, rheumatoid arthritic, degenerative knees, and knees with Reiter's syndrome showed that their respective waveforms, spectral patterns, and statistical properties are, on the average, unique and proved to be a promising nondestructive diagnostic tool for early detection of the type and extent of knee joint damage.
Lineage Tracing Evidence for In Vitro Dedifferentiation but Rare Proliferation of Mouse Pancreatic β-Cells (2007)

Understanding and manipulating pancreatic beta-cell proliferation is a major challenge for pancreas biology and diabetes therapy. Recent studies have raised the possibility that human beta-cells can undergo dedifferentiation and give rise to highly proliferative mesenchymal cells, which retain the potential to redifferentiate into beta-cells. To directly test whether cultured beta-cells dedifferentiate, we applied genetic lineage tracing in mice. Differentiated beta-cells were heritably labeled using the Cre-lox system, and their fate in culture was followed. We provide evidence that mouse beta-cells can undergo dedifferentiation in vitro into an insulin-, pdx1-, and glut2-negative state. However, dedifferentiated beta-cells only rarely proliferate under standard culture conditions and are eventually eliminated from cultures. Thus, the predominant mesenchymal cells seen in cultures of mouse islets are not of a beta-cell origin.
Adverse Events Associated with Childhood Vaccines (1994)

Childhood immunization is one of the major public health measures of the 20th century and is now receiving special attention from the Clinton administration. At the same time, some parents and health professionals are questioning the safety of vaccines because of the occurrence of rare adverse events after immunization.This volume provides the most thorough literature review available about links between common childhood vaccines--tetanus, diphtheria, measles, mumps, polio, Haemophilus influenzae b, and hepatitis B--and specific types of disorders or death.The authors discuss approaches to evidence and causality and examine the consequences--neurologic and immunologic disorders and death--linked with immunization. Discussion also includes background information on the development of the vaccines and details about the case reports, clinical trials, and other evidence associating each vaccine with specific disorders.This comprehensive volume will be an important resource to anyone concerned about the immunization controversy: public health officials, pediatricians, attorneys, researchers, and parents.
Soybean seed lectin gene and flanking nonseed protein genes are developmentally regulated in transformed tobacco plants. (1986)

We introduced a 17.1-kilobase soybean DNA fragment containing the lectin gene and at least four nonseed protein genes into the tobacco genome. As in soybean plants, lectin mRNA is present in tobacco seeds, accumulates and decays during tobacco seed development, and is translated into a protein that accumulates prior to dormancy. Each soybean nonseed protein mRNA is present in tobacco leaves, roots, stems, and seeds at levels similar to that found in soybean plants. We conclude that a differentially expressed soybean gene cluster is correctly regulated in transformed tobacco plants and that sequences controlling their expression are recognized by regulatory factors present in tobacco cells.
Inhibition of angiogenesis by suramin. (1992)

In this study, we have determined the ability of suramin to inhibit angiogenesis in the chick chorioallantoic membrane. Suramin alone showed significant angiostatic activity in a dose-related manner. Suramin also potentiated the activity of the angiostatic steroids, cortisol-21-phosphate, 17 alpha-hydroxyprogesterone, tetrahydrocortisol, and tetrahydrocortexolone. The presence of heparin decreased the angiostatic activity of suramin. These results suggest that suramin may decrease tumor growth by inhibiting angiogenesis. These novel findings indicate that suramin, perhaps in combination with angiostatic steroids, may be the basis for important new therapeutic approaches for diseases of neovascularization.
A fatty acid‐binding protein 7/RXRβ pathway enhances survival and proliferation in triple‐negative breast cancer (2012)

FABP7 has been implicated in tumour cell proliferation, cell migration, and poor prognosis in patients with high-grade astrocytoma and melanoma. In this study, we examine FABP7 expression in a cohort of 176 primary breast cancers by gene profiling and tissue microarray immunostaining. We show that FABP7 is significantly up-regulated in triple-negative breast cancer. Elevated FABP7 levels are associated with poor prognosis, absence of oestrogen and progesterone hormone receptors (ER, PR) and HER2, increased cell proliferation, and high tumour grade. Depletion of FABP7 in the ER/PR-negative cell line, MDA-MB-435S, significantly reduced cell growth rate and sensitized the cells to growth inhibition by omega-3 docosahexaenoic acid (DHA). A target of DHA-bound FABP7 in the nucleus is RXRβ, a retinoid-activated nuclear receptor that functions as a transcription factor by either homodimerizing or heterodimerizing with other nuclear receptors such as PPARs. Based on our microarray data, RXRβ, like FABP7, is an adverse prognostic factor for breast cancer. We propose that the DHA-FABP7-RXRβ pathway promotes cell survival/proliferation in triple-negative breast cancer. Targeting this pathway may thus provide an alternate route for the treatment of triple-negative breast cancer.
CD14 is involved in control of human immunodeficiency virus type 1 expression in latently infected cells by lipopolysaccharide. (1992)

Lipopolysaccharide (LPS) potently stimulates human immunodeficiency virus type 1 (HIV-1) long terminal repeat-directed transcription in transfected monocyte-macrophage cell lines and dramatically increases HIV-1 production in the latently infected monocyte-macrophage-like cell line U1. This response to LPS, however, can only be observed after pretreatment of the U1 cells with granulocyte-macrophage colony-stimulating factor (GM-CSF). CD14, the differentiation antigen that acts as a receptor for complexes of LPS and LPS-binding protein, is now demonstrated to be involved in LPS-induced stimulation of HIV-1 replication. CD14 is shown to be expressed on a subpopulation of U1 cells only after treatment with GM-CSF and correlates with HIV-1 production stimulated by LPS. Importantly, only those U1 cells that express CD14 can be induced by LPS to upregulate HIV-1 production. In addition, a monoclonal antibody directed against CD14 can block LPS-induced stimulation of HIV-1 production from these latently infected cells.