gebhart/patrepeval huggingface dataset:
Experimental brown spider bite in the guinea pig: Results of treatment with dapsone or hyperbaric oxygen (1994)

The brown recluse spider (Loxosceles reclusa) is a common inhabitant of the central United States. Loxoceles envenomation is a cause of significant medical problems, causing a necrotic skin lesion that often becomes a problem wound. Treatment of the skin lesion is controversial, with no current widely accepted therapy. The effects of treatment with hyperbaric oxygen or dapsone on brown spider bites were studied using guinea pigs. Progression and healing of experimental brown spider bites were measured in animals treated or pretreated with dapsone or hyperbaric oxygen and in controls. There was a significant decrease in lesion size in animals pretreated with hyperbaric oxygen at days 3,5, and 6 as compared to dapsone-pretreated animals or untreated controls. However, there were no differences noted between groups when treatment was begun 36 h after envenomation. There was a nonsignificant increase ( p = 0.12) noted in myeloperoxidase levels from lesions of animals pretreated with hyperbaric oxygen. No other differences in levels of catalase, glutathione, or superoxide dismutase activity were noted between treatment groups. These results suggest that hyperbaric oxygen may have potential in the treatment of brown spider bites; however, further study is necessary to prove its efficacy.
Deterministic Lateral Displacement: The Next-Generation CAR T-Cell Processing? (2018)

Reliable cell recovery and expansion are fundamental to the successful scale-up of chimeric antigen receptor (CAR) T cells or any therapeutic cell-manufacturing process. Here, we extend our previous work in whole blood by manufacturing a highly parallel deterministic lateral displacement (DLD) device incorporating diamond microposts and moving into processing, for the first time, apheresis blood products. This study demonstrates key metrics of cell recovery (80%) and platelet depletion (87%), and it shows that DLD T-cell preparations have high conversion to the T-central memory phenotype and expand well in culture, resulting in twofold greater central memory cells compared to Ficoll-Hypaque (Ficoll) and direct magnetic approaches. In addition, all samples processed by DLD converted to a majority T-central memory phenotype and did so with less variation, in stark contrast to Ficoll and direct magnetic prepared samples, which had partial conversion among all donors (<50%). This initial comparison of T-cell function infers that cells prepared via DLD may have a desirable bias, generating significant potential benefits for downstream cell processing. DLD processing provides a path to develop a simple closed system that can be automated while simultaneously addressing multiple steps when there is potential for human error, microbial contamination, and other current technical challenges associated with the manufacture of therapeutic cells.
Aqueous redox polymerization of acrylamide initiated by citric acid/permanganate (1976)

Abstract The homogeneous redox polymerization of acrylamide by the citric acid/permanganate initiating system was investigated at 35±0,2°C under nitrogen. Variation of the activator concentration in the range of 2,5.10 −3 to 20,0.10 −3 mol/dm 3 had no effect on the rate of polymerization. The initial rate increased with the increasing catalyst concentration and a value of nearly unity of the catalyst exponent confirmed a unimolecular chain termination process. The rate varied linearly with the monomer concentration over a wide range (5,0.10 −2 to 20,0.10 −2 mol/dm 3 ). The initial rate increased with the increasing temperature but the maximum conversion showed a decrease as the temperature was raised above 35°C. The energy of activation, in the temperature range of 25 to 45°C, was found to be 55,90kJ/mol (13,36 kcal/mol). The addition of neutral salts, Co(NO 3 ) 2 , Ni(NO 3 ) 2 , organic solvents, and complexing agents, all water soluble, reduced the rate and percentage of conversion. The introduction of MnSO 4 or of more catalyst (at intermediate stages) into the system increased both the rate and limiting conversion.
Probiotics in marine larviculture (2006)

Owing to the problem of antibiotic resistance and subsequent reluctance of using antibiotics, the use of probiotics in larviculture is becoming increasingly popular. During the early stages of development, manipulation of the larval digestive system seems possible through the addition of probiotics either through the culture water or via the livefood. Well-studied probiotics used in human medicine and terrestrial agriculture have proved to be successful in aquaculture and therefore reduce the need for extensive biosafety trials. The selection of probiotics requires various in vitro screening experiments, which assay for the production of antagonist compounds, their growth in and attachment to fish intestinal mucus, and the production of other beneficial compounds such as vitamins, fatty acids and digestive enzymes. Further information regarding probiont suitability can be obtained from its identification, interaction with livefood and host pathogenicity. Finally, pilot-scale in vivo tests need be performed, after which a production cost-benefit analysis to determine its economic viability needs to be undertaken.
A centralized gene-based HIV-1 vaccine elicits broad cross-clade cellular immune responses in rhesus monkeys (2008)

One of the major challenges that must be met in developing an HIV-1 vaccine is devising a strategy to generate cellular immunity with sufficient breadth to deal with the extraordinary genetic diversity of the virus. Amino acids in the envelopes of viruses from the same clade can differ by >15%, and those from different clades can differ by >30%. It has been proposed that creating immunogens using centralized HIV-1 gene sequences might provide a practical solution to this problem. Such centralized genes can be generated by employing a number of different strategies: consensus, ancestral, or center of tree sequences. These computer-generated sequences are a shorter genetic distance from any two contemporary virus sequences than those contemporary sequences are from each other. The present study was initiated to evaluate the breadth of cellular immunity generated through immunization of rhesus monkeys with vaccine constructs expressing either an HIV-1 global consensus envelope sequence (CON-S) or a single patient isolate clade B envelope sequence (clade B). We show that vaccine immunogens expressing the single centralized gene CON-S generated cellular immune responses with significantly increased breadth compared with immunogens expressing a wild-type virus gene. In fact, CON-S immunogens elicited cellular immune responses to 3- to 4-fold more discrete epitopes of the envelope proteins from clades A, C, and G than did clade B immunogens. These findings suggest that immunization with centralized genes is a promising vaccine strategy for developing a global vaccine for HIV-1 as well as vaccines for other genetically diverse viruses.
Proper Costimulation of Tumor-Reactive T Lymphocytes May Provide a Key to Unlock Their Antitumor Activity (2003)

Commentary to: Tumor Expression of 4-1BB Ligand Sustains Tumor Lytic T CellsHua Zhang, Melinda S. Merchant, Kevin S. Chua, Chand Khanna, Lee J. Helman, Bill Telford, Yvona Ward, Jeffrey Summers, Jeff Toretsky, Elaine K. Thomas, Carl H. June and Crystal L. Mackall
Effects of a Dopamine Agonist on the Pharmacodynamics of Levodopa in Parkinson Disease (2010)

Pramipexole augmented the motor response to levodopa beyond a simple additive effect and increased the severity of levodopa-induced dyskinesia. When considering a combination of these therapies, an appropriate balance should be maintained regarding gain of motor function vs worsening of dyskinesia. Trial Registration clinicaltrials.gov Identifier: NCT00666653.
Analysis of major antigens of Haemophilus (Actinobacillus) pleuropneumoniae and related organisms (1987)

Outer membrane protein (OMP)-enriched extracts and whole-cell protein preparations of Haemophilus (Actinobacillus) pleuropneumoniae and related organisms were examined by polyacrylamide gel electrophoresis and immunoblotting. Both the OMP-enriched and whole-cell protein profiles of Actinobacillus suis, A. pleuropneumoniae (NAD-independent biovar), A. lignieresii, and Pasteurella haemolytica were very similar to those of H. pleuropneumoniae serotypes 1 to 8. Antisera prepared against H. pleuropneumoniae typically recognized three major OMP antigens with approximate molecular weights of 17,000 (17K), 32K, and 42K in immunoblots of H. pleuropneumoniae serotypes 1 to 8, Actinobacillus spp., and P. haemolytica. Antisera prepared against Actinobacillus spp. and Haemophilus sp. "minor group" also recognized these 17K, 32K, and 42K antigens. Using absorbed sera, we demonstrated that the 17K antigen had an epitope (or epitopes) common to all the gram-negative organisms examined, including Escherichia coli. The 32K and 42K antigens had epitopes common to members of the family Pasteurellaceae but, in the case of the 32K antigen, also contained unique epitopes. These results provide a basis for understanding the lack of specificity of serodiagnostic tests for H. pleuropneumoniae infection and provide another line of evidence for the association of H. pleuropneumoniae with the genus Actinobacillus.
A rare-cell detector for cancer (2004)

Although a reliable method for detection of cancer cells in blood would be an important tool for diagnosis and monitoring of solid tumors in early stages, current technologies cannot reliably detect the extremely low concentrations of these rare cells. The preferred method of detection, automated digital microscopy (ADM), is too slow to scan the large substrate areas. Here we report an approach that uses fiber-optic array scanning technology (FAST), which applies laser-printing techniques to the rare-cell detection problem. With FAST cytometry, laser-printing optics are used to excite 300,000 cells per sec, and emission is collected in an extremely wide field of view, enabling a 500-fold speed-up over ADM with comparable sensitivity and superior specificity. The combination of FAST enrichment and ADM imaging has the performance required for reliable detection of early-stage cancer in blood.
Bimetallic redox sites for photochemical CO2 splitting in mesoporous silicate sieve (2005)

Binuclear redox sites consisting of a Ti or Zr oxo-bridged to a Cu(I) or Sn(II) center have been covalently anchored on the pore surface of MCM-41 silicate sieve by a stepwise grafting procedure. The bimetallic sites feature a metal-to-metal charge-transfer (MMCT) absorption extending deep into the visible region. Covalent anchoring is manifested by low-frequency infrared modes. Irradiation of the MMCT chromophore of ZrCu(I)–MCM-41 loaded with 1 atm of CO 2 gas at RT resulted in growth of CO (2150 cm –1 ) and H 2 O (1600 cm –1 ). Photolysis experiments using 13 CO 2 and C 18 O 2 demonstrate that carbon monoxide and the oxygen atom of the water product originate from CO 2 , indicating splitting of the CO 2 upon excitation of the MMCT moiety. This is the first observation of CO 2 photoreduction at a binuclear MMCT site at the gas-solid interface. .
Development of an extruder-feeder biomass direct liquefaction process (1991)

As an abundant, renewable, domestic energy resource, biomass could help the United States reduce its dependence on imported oil. Biomass is the only renewable energy technology capable of addressing the national need for liquid transportation fuels. Thus, there is an incentive to develop economic conversion processes for converting biomass, including wood, into liquid fuels. Through research sponsored by the US DOE's Biomass Thermochemical Conversion Program, the University of Arizona has developed a unique biomass direct liquefaction system. The system features a modified single-screw extruder capable of pumping solid slurries containing as high as 60 wt% wood flour in wood oil derived vacuum bottoms at pressures up to 3000 psi. The extruder-feeder has been integrated with a unique reactor by the University to form a system which offers potential for improving high pressure biomass direct liquefaction technology. The extruder-feeder acts simultaneously as both a feed preheater and a pumping device for injecting wood slurries into a high pressure reactor in the biomass liquefaction process. An experimental facility was constructed and following shakedown operations, wood crude oil was produced by mid-1985. By July 1988, a total of 57 experimental continuous biomass liquefaction runs were made using White Birch wood feedstock. Good operability was achieved at slurry feed rates up to 30 lb/hr, reactor pressures from 800 to 3000 psi and temperatures from 350{degree}C to 430{degree}C under conditions covering a range of carbon monoxide feed rates and sodium carbonate catalyst addition. Crude wood oils containing as little as 6--10 wt% residual oxygen were produced. 38 refs., 82 figs., 26 tabs.
Activating Mutations in Cytokine Receptors: Implications for Receptor Function and Role in Disease (1997)

RECEPTORS FOR MANY cytokines and growth factors are triggered by the binding of their cognate ligands to assemble into an active multi-subunit complex that frequently comprises a homodimer of the ligand-binding subunit. This results in the activation of intracellular protein tyrosine kinase
Advances toward Gene Therapy for Hemophilia at the Millennium (1999)

Hemophilia A and hemophilia B are both X chromosome-linked recessive bleeding disorders that affect about 1 in 5000 males and result from a deficiency in the coagulation factors VIII and IX, respectively. Severely affected individuals require frequent administration of factor VIII or factor IX preparations derived from human plasma, and more recently, from recombinant DNA technology. Although these preparations have greatly reduced the contamination with blood-borne pathogens, there still exist significant limitations with protein replacement therapy. As we elucidate more about the expression, structure, and function of these coagulation factors new avenues will open for the development of novel genetically improved coagulation factors. Several aspects of the hemophilias A and B make these diseases attractive candidates for gene therapy. These advantages include the following: (1) these proteins are readily delivered into the circulation from a variety of different cell types; (2) low levels of expression would significantly improve the management of bleeding episodes in these patients; (3) it is unlikely that regulated expression of these proteins will be required; and (4) there are excellent animal models for these diseases. Although progress with gene transfer of factor IX has proceeded at a greater rate than factor VIII, success with both molecules has demonstrated partial persistent correction in mouse and canine models of hemophilia A and B. The information gained from these animal studies has provided new insights into gene therapeutic approaches for genetic diseases. In addition, several gene therapy clinical studies for the treatment of hemophilia A and B were initiated in 1999.
Monoclonal antibody G6K12 specific for membrane‐associated differentiation marker of human stratified squamous epithelia and squamous cell carcinoma (1993)

Monoclonal antibody (G6K12) specific for differentiated keratinocytes was developed using in vitro immunization against the SCC-25 cell line. G6K12 only recognized stratified portions of cultured SCC-25 cells. Immunohistochemical examination using normal human oral mucosa showed that specific G6K12-reactivity was limited to the lower spinous-cell layers, while this antibody weakly bound to cells in basal-cell layers as well as in the upper spinous, granular and cornified-cell layers. G6K12 was also reactive to keratinocytes in most moderately- and well-differentiated SCC tissues. Immunoelectron microscopic examination further demonstrated that the G6K12-immunoreactive area was at the outer surface of the entire plasma membrane, including the microvilli of stratified SCC-25. G6K12-binding was reduced 50% by the treatment of native cells with glycoendoceramidase for 2 h. These results suggest that G6K12 recognizes a plasma membrane-anchored glycoconjugate which is specific for differentiated keratinocytes.
Total Knee Arthroplasty (1985)

Over the past 15 years, total knee arthroplasty has evolved to a substantial degree with respect to both design and technique. Although initially it lagged well behind the "state of the art" for total hip arthroplasty, it now appears to have surpassed the older procedure, particularly with respect to durability. The management of severe deformities and the improvement of the eventual range of motion remain challenges, but both are being met with increasing success.
Interictal spike analysis using stochastic point process (2004)

Interictal spikes are important indicators of epileptic focus (foci). The spiky events in EEG waveforms recorded from different regions of the brain provide important information about the dynamic transitions of epileptic activity. We extract sequences of spikes, called spike trains, from individual EEG channels and evaluate them using the stochastic cross-correlogram, where the time of occurrence of each spike in a single channel is statistically correlated to the times of occurrences of spikes in other channels. A probability density function (pdf) is constructed which provides a distribution of the time intervals between the appearances of spike train events across a pair of channels. The time interval corresponding to the maximum of the pdf indicates a likelihood of latency between the two spiky events during the specified observation period. The higher the peak value, the stronger the cross-correlation between the two spike trains. We have applied the cross-correlogram analysis to subdural EEC data recorded from multiple electrodes spread over different regions of the brain. We calculated the maximum peak interval of the cross-correlogram between every pair of spike trains to observe the dynamical transitions of the interictal activity in the temporal and spatial domain. Our results show that, by inspecting the dynamic developments of spike events in these domains, a powerful tool is obtained for the diagnosis of epileptic focus (foci)
Recombinant Modified Vaccinia Virus Ankara Expressing a Soluble Form of Glycoprotein B Causes Durable Immunity and Neutralizing Antibodies against Multiple Strains of Human Cytomegalovirus (2004)

Human cytomegalovirus (CMV) is a viral pathogen that infects both genders, who remain asymptomatic unless they receive immunosuppressive drugs or acquire infections that cause reactivation of latent virus. CMV infection also causes serious birth defects following primary maternal infection during gestation. A safe and effective vaccine to limit disease in this population continues to be elusive. A well-studied antigen is glycoprotein B (gB), which is the principal target of neutralizing antibodies (NAb) towards CMV in humans and has been implicated as the viral partner in the receptor-mediated infection by CMV in a variety of cell types. Antibody-mediated virus neutralization has been proposed as a mechanism by which host immunity could modify primary infection. Towards this goal, an attenuated poxvirus, modified vaccinia virus Ankara (MVA), has been constructed to express soluble CMV gB (gB680-MVA) to induce CMV NAb. Very high levels of gB-specific CMV NAb were produced after two doses of the viral vaccine. NAb were durable within a twofold range for up to 6 months. Neutralization titers developed in immunized mice are equivalent to titers found clinically after natural infection. This viral vaccine, expressing gB derived from CMV strain AD169, induced antibodies that neutralized CMV strains of three different genotypes. Remarkably, preexisting MVA and vaccinia virus (poxvirus) immunity did not interfere with subsequent immunizations of gB680-MVA. The safety characteristics of MVA, combined with the robust immune response to CMV gB, suggest that this approach could be rapidly translated into the clinic.
N-Terminal Extension of Human Immunodeficiency Virus Capsid Protein Converts the In Vitro Assembly Phenotype from Tubular to Spherical Particles (1998)

Expression of retroviral Gag polyproteins is sufficient for morphogenesis of virus-like particles with a spherical immature protein shell. Proteolytic cleavage of Gag into the matrix (MA), capsid (CA), nucleocapsid (NC), and p6 domains (in the case of human immunodeficiency virus [HIV]) leads to condensation to the mature cone-shaped core. We have analyzed the formation of spherical or cylindrical particles on in vitro assembly of purified HIV proteins or inside Escherichia coli cells. CA protein alone yielded cylindrical particles, while all N-terminal extensions of CA abolished cylinder formation. Spherical particles with heterogeneous diameters or amorphous protein aggregates were observed instead. Extending CA by 5 amino acids was sufficient to convert the assembly phenotype to spherical particles. Sequences C-terminal of CA were not required for sphere formation. Proteolytic cleavage of N-terminally extended CA proteins prior to in vitro assembly led to the formation of cylindrical particles, while proteolysis of in vitro assembly products caused disruption of spheres but not formation of cylinders. In vitro assembly of CA and extended CA proteins in the presence of cyclophilin A (CypA) at a CA-to-CypA molar ratio of 10:1 yielded significantly longer cylinders and heterogeneous spheres, while higher concentrations of CypA completely disrupted particle formation. We conclude that the spherical shape of immature HIV particles is determined by the presence of an N-terminal extension on the CA domain and that core condensation during virion maturation requires the liberation of the N terminus of CA.
Channel Estimation and Performance Analysis of One-Bit Massive MIMO Systems (2017)

This paper considers channel estimation and system performance for the uplink of a single-cell massive multiple-input multiple-output system. Each receiver antenna of the base station is assumed to be equipped with a pair of one-bit analog-to-digital converters to quantize the real and imaginary part of the received signal. We first propose an approach for channel estimation that is applicable for both flat and frequency-selective fading, based on the Bussgang decomposition that reformulates the nonlinear quantizer as a linear function with identical first- and second-order statistics. The resulting channel estimator outperforms previously proposed approaches across all SNRs. We then derive closed-form expressions for the achievable rate in flat fading channels assuming low SNR and a large number of users for the maximal ratio and zero forcing receivers that takes channel estimation error due to both noise and one-bit quantization into account. The closed-form expressions, in turn, allow us to obtain insight into important system design issues such as optimal resource allocation, maximal sum spectral efficiency, overall energy efficiency, and number of antennas. Numerical results are presented to verify our analytical results and demonstrate the benefit of optimizing system performance accordingly.