gebhart/patrepeval huggingface dataset:
Rapid chromatographic technique for preparative separations with moderate resolution (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRapid chromatographic technique for preparative separations with moderate resolutionW. Clark Still, Michael Kahn, and Abhijit MitraCite this: J. Org. Chem. 1978, 43, 14, 2923–2925Publication Date (Print):July 1, 1978Publication History Published online1 May 2002Published inissue 1 July 1978https://doi.org/10.1021/jo00408a041RIGHTS & PERMISSIONSArticle Views50070Altmetric-Citations5333LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (399 KB) Get e-Alerts Get e-Alerts
RISK FACTORS FOR GRAFT FAILURE DUE TO RECURRENT HEPATITIS C AFTER LIVER TRANSPLANTATION. (2004)

O367 Aims: Recurrence of hepatitis C (HCV)-infection after liver transplantation (OLT) in HCV positive patients is almost universal. Severity of graft hepatitis increases during the long term follow-up and up to 30% of patients develop severe graft hepatitis and cirrhosis. However, there are still no clear predictors for severe recurrence. The aim of this study was to examine the 10-years outcome and risk factors for graft failure due to HCV recurrence. Methods: In a prospective analysis 232 OLT‘s in 209 HCV positive patients with a median age of 53 years were analyzed. Immunosuppression was based on Cyclosporine A or Tacrolimus in different protocols. Predictors for outcome were genotype, viremia, donor variables, recipient demographics, immunosuppression, year of transplant, and HLA compatibilities. Serial biopsies were performed 1-, 3-, 5-, 7-, and 10 years after OLT. Results: Actuarial 5-, and 10-year patient survival was 75.8% and 68.8%. 28/81 (35%) graft losses were related to HCV recurrence. 10/28 patients suffered from severe cholestatic hepatitis and the remaining 18 patients developed graft loss due to recurrent cirrhosis. Significant risk factors for recurrent cirrhosis were donor age > 33 years and 1 or more HLA matches. Female donor gender and multiple steroid pulse therapies contributed to an increased risk for the development of severe cholestatic hepatitis. The overall fibrosis progression rate was not linear. The fibrosis scores were 1.2 after one, 1.7 after three, 1.9 after five, 2.1 after 7 seven and 2.2 after ten years. The 39 recipients with fibrosis stages 3 or 4 in the 1-year biopsy had a significantly reduced survival rate, while fibrosis stage 0-2 indicated excellent survival. Independent risk factors for progression to fibrosis at one year were HCV genotype 1 and 4 (p=0.01) and donor age>33 years (p=0.01). Conclusions: Our data show that long-term survival in hepatitis C positive patients is similar compared to other indications. However, 35% of the graft losses are related to the underlying disease. In these patients we have to divide in different forms of recurrent hepatitis C with different risk factors. Our analysis show that recurrent cirrhosis is common in older donors and related to HLA dependent immunological response of the recipient. In contrast to this cholestatic fibrosing occurs in transplants with female donors receiving multiple steroid pulse therapies indicating overimmunosuppression. This data provide information on the course of recurrent hepatitis C and may be helpful to individualize the treatment of transplanted patients.
The mast cell binding site on human immunoglobulin E (1988)

Antibodies of the immunoglobulin E isotype sensitize mast cells and basophils for antigen-induced mediator release by binding through the Fc portion to a high-affinity receptor (Fc epsilon R1, Ka = 10(9)M-1) on the cell surface causing the clinical manifestations of type I hypersensitivity. As the amino acid sequence of the human epsilon chain is now known, attempts have been made to map the Fc epsilon R1 binding site on IgE to a fragment smaller than Fc epsilon using proteolytic cleavage products, none of which proved to be active. Cleavage between the C epsilon 2 and C epsilon 3 domains released two inactive fragments, suggesting that the junction between these segments could be important in receptor binding. This region is protected against protease digestion in the rat IgE complex with the receptor of rat basophilic leukaemia cells. Here we report the mapping of the mast cell receptor binding site on human IgE to a sequence of 76 amino acids at the C epsilon 2/C epsilon 3 junction. Recombinant peptides containing this sequence inhibit passive sensitization of skin mast cells in vivo and sensitize mast cells to degranulation by anti-IgE in vitro almost as efficiently as a myeloma IgE. Fragments containing the separate domains are inactive. Additional sequences are required for rapid assembly of fragments into disulphide-linked dimers, suggesting that a single chain can form the active site. In a three-dimensional model of the human Fc epsilon, the two identical segments are far apart. Each folds to generate a cleft between the C epsilon 2 and C epsilon 3 domains on the surface of the Fc epsilon. The docking of IgE on to mast cells could take place within this cleft.
Digital Supply Chain Management in the Videogames Industry: A Systematic Literature Review (2020)

Abstract As industries mature, they rely more heavily on supply chain management (SCM) to ensure effective operations leading to greater levels of organisational performance. SCM has been widely covered in many industrial areas and, in line with other burgeoning sectors such as Tourism, an industry focus provides the opportunity to look in-depth at the context-based factors that affect SCM. Developments in digital distribution and rapid technological innovations have resulted in an increased focus on Digital Supply Chains (DSCs), which bring about significant changes to how consumers, customers, suppliers, and manufacturers interact, affecting supply chain design and processes. Through a systematic review of the Videogames Industry Supply Chain Management literature, which serves as a pertinent contextual example of a DSC, we look at how supply chains are affected by structural, market and technological change, such as increased platformisation, disintermediation and the proliferation of digital distribution. We distil these findings into a new research agenda, which identifies themes in line with extant DSC research, provides a series of relevant practice recommendations and identifies opportunities for future research.
Fiber optic sensor for calcium(2+) based on an induced change in the conformation of the protein calmodulin (1994)

A fiber optic sensor that exploits the natural selectivity of the Ca(2+)-binding protein calmodulin (CaM) is described. In this sensor, a dialysis membrane is used to entrap a fluorescein-labeled CaM (F-CaM) solution at the common end of a bifurcated fiber optic bundle. Ca2+ ions in a sample solution can diffuse through the membrane and bind to the F-CaM. Upon binding with Ca2+, CaM undergoes a conformational change that induces a change in the fluorescence of the attached fluorescein tag. This change in fluorescence can be related to the concentration of Ca2+ in the sample solution. The detection limit for the sensor is 5 x 10(-8) M Ca2+. The sensor has no interference by Mg2+ at concentrations as high as 10(-2) M.
Identifying and modifying protein-DNA and protein-protein interactions using a bacterial two-hybrid selection system (2001)

A bacterial two-hybrid system based on transcriptional activation in E. coli has recently been described. A variety of different protein-DNA and protein-protein interactions from bacteria, yeast, and humans have been studied using this bacterial-based system. The method, because it is based in bacteria, offers significant advantages relative to its yeast counterpart including the ability to analyze complex libraries > 10(8) in size, ease of use, and speed. The ability to easily and rapidly process very large libraries make this system a powerful tool for identifying, modifying, or optimizing protein-DNA and protein-protein interactions.
ATP site-directed competitive and irreversible inhibitors of protein kinases (2000)

Several tyrosine and serine/threonine protein kinases have emerged in the last few years as attractive targets in the search for new therapeutic agents being applicable in many different disease indications. Initially, inhibition of these protein kinases by ATP site-directed inhibitors was considered less prone to success, but medicinal chemists from both academia and industry have been able to impart potency and selectivity to a limited number of scaffolds by modulating and fine-tuning the interactions of the modified template with the ATP binding site of the selected kinase. The chemical templates that have been used in the synthesis of ATP site-directed protein kinase inhibitors are reviewed with emphasis on the kinase inhibitors that have entered or are about to enter clinical trials. Examples have been selected to illustrate how structure-based design approaches and new methods to increase compound diversity have had an impact on this area of research.
Core-shell silicon nanowire solar cells (2013)

Silicon nanowires can enhance broadband optical absorption and reduce radial carrier collection distances in solar cell devices. Arrays of disordered nanowires grown by vapor-liquid-solid method are attractive because they can be grown on low-cost substrates such as glass, and are large area compatible. Here, we experimentally demonstrate that an array of disordered silicon nanowires surrounded by a thin transparent conductive oxide has both low diffuse and specular reflection with total values as low as < 4% over a broad wavelength range of 400 nm < λ < 650 nm. These anti-reflective properties together with enhanced infrared absorption in the core-shell nanowire facilitates enhancement in external quantum efficiency using two different active shell materials: amorphous silicon and nanocrystalline silicon. As a result, the core-shell nanowire device exhibits a short-circuit current enhancement of 15% with an amorphous Si shell and 26% with a nanocrystalline Si shell compared to their corresponding planar devices.
Adenovirus-mediated transfer of the p53 gene produces rapid and generalized death of human glioma cells via apoptosis. (1996)

Wild-type p53 is involved in several aspects of cell cycle control and suppression of transformation, inducing either apoptosis or G1 block in cell cycle progression. Using a recombinant adenovirus containing the wild-type p53 cDNA, the biological effects of the newly expressed wild-type p53 protein were examined in six human glioma cell lines. Three cell lines (U-251 MG, U-373 MG, and A-172) expressed endogenous mutant p53, and the other three (U-87 MG, EFC-2, and D54 MG) expressed wild-type p53. The restoration of normal p53-encoded protein in the mutant cell lines induced apoptosis as assessed by morphological studies using nuclear staining, electron microscopy, and flow cytometric assays. In wild-type p53 cell lines, however, the overexpression of wild-type p53 did not result in apoptosis but inhibited cellular proliferation rather drastically and modified the neoplastic phenotype. Differential effects suggest two pathways for glioma oncogenesis and a possible therapeutic strategy.
Ureteral endometriosis: A case report (2017)

A case of endometriosis of ureter in the Department of Gynaecology and Obstetrics of the Third Affiliated Hospital of Inner Mongolia Medical School was collected and analyzed on the basis of diagnosis, physical examination and treatment. The disease was rare due to its atypical clinical symptoms so that it was difficult to diagnose. The paper aims to expand our thinking and improve the gynecologists awareness and the level of diagnosis and treatment of rare diseases through the clinical analysis of the case.
An architecture for privacy-sensitive ubiquitous computing (2004)

Privacy is the most often-cited criticism of ubiquitous computing, and may be the greatest barrier to its long-term success. However, developers currently have little support in designing software architectures and in creating interactions that are effective in helping end-users manage their privacy. To address this problem, we present Confab, a toolkit for facilitating the development of privacy-sensitive ubiquitous computing applications. The requirements for Confab were gathered through an analysis of privacy needs for both end-users and application developers. Confab provides basic support for building ubiquitous computing applications, providing a framework as well as several customizable privacy mechanisms. Confab also comes with extensions for managing location privacy. Combined, these features allow application developers and end-users to support a spectrum of trust levels and privacy needs.
Deletion of chromosome 11p15, p12, q22, q23-24 loci in human prostate cancer (1997)

Loss of heterozygosity (LOH) on chromosome 11 is frequently altered in various epithelial cancers. The present study was designed to investigate LOH on chromosome 11 in microdissected samples of normal prostatic epithelium and invasive carcinoma from the same patients. For this purpose, DNA was extracted from the microdissected normal and tumor cells of 38 prostate cancers, amplified by polymerase chain reaction PCR and analyzed for LOH on chromosome 11 using 9 different polymorphic DNA markers (D11S1307, D11S989, D11S1313, D11S898, D11S940, D11S1818, D11S924, D11S1336 and D11S912). LOH on chromosome 11 was identified in 30 of 38 cases (78%) with at least one marker. Four distinct regions of loss detected were: 1) at 11p15, at loci between D11S1307 and D11S989; 2) at 11p12, on locus D11S131 (11p12); 3) at 11q22, on loci D11S898, D11S940 and D11S1818; and 4) at 11q23-24, on loci between D11S1336 and D11S912. We found 25% of the tumors with LOH at 11p15; 39% had LOH at 11p12; 66% had LOH at 11q22; and 47% had LOH at 11q23-24. These deletions at 11p15, 11p12, 11q22 and 11q23-24 loci were not related to the stage or grade of the tumor.
Coordinate occupancy of AP-1 sites in the vitamin D-responsive and CCAAT box elements by Fos-Jun in the osteocalcin gene: model for phenotype suppression of transcription. (1990)

Osteocalcin, a bone-specific protein and marker of the mature osteoblast, is expressed only in nonproliferating osteoblasts in a mineralizing extracellular matrix, while type I collagen is expressed in proliferating cells. The nuclear proteins encoded by the c-fos and c-jun protooncogenes are expressed during the proliferation period of osteoblast phenotype development. We present evidence that AP-1 (HeLa cell-activating protein 1) sites residing within two promoter elements of the osteocalcin gene bind the Fos-Jun protein complex: the osteocalcin box (OC box; nucleotides -99 to -76), which contains a CCAAT motif as a central element and influences tissue-specific basal levels of osteocalcin gene transcription, and the vitamin D-responsive element (VDRE; nucleotides -462 to -440), which mediates enhancement of osteocalcin gene transcription. Gel electrophoretic mobility-shift analysis demonstrated high AP-1 binding activity in proliferating osteoblasts and dramatic changes in this activity after the down-regulation of proliferation and the initiation of extracellular-matrix mineralization in primary cultures of normal diploid osteoblasts. Methylation interference analysis established at single nucleotide resolution that purified recombinant Fos and Jun proteins bind in a sequence-specific manner to the AP-1 sites within the VDRE and OC box. Similarly, an AP-1 motif within a putative VDRE of the alkaline phosphatase gene, which is also expressed after the completion of proliferation, binds the Fos-Jun complex. These results support a model in which coordinate occupancy of the AP-1 sites in the VDRE and OC box in proliferating osteoblasts may suppress both basal level and vitamin D-enhanced osteocalcin gene transcription as well as transcription of other genes associated with osteoblast differentiation--a phenomenon we describe as phenotype suppression. This model is further supported by binding of the Fos-Jun complex at an AP-1 site in the type alpha I collagen promoter that is contiguous with, but not overlapping, the VDRE. Such a sequence organization in the collagen VDRE motif is compatible with vitamin D modulation of collagen but not with osteocalcin and alkaline phosphatase expression in proliferating osteoblasts.
Patterns of ethanol‐induced cell death in the developing nervous system of mice; Neural fold states through the time of anterior neural tube closure (1992)

Vital staining and routine histological analyses of mouse embryos 12 h after acute maternal ethanol administration (2.9 g/kg) illustrated that selected neuronal cell populations are killed. At the time of treatment, embryos had 5-15 somite pairs, corresponding to the developmental stages occurring in humans during the fourth week of post-fertilization; i.e. when neural folds are present and neural tube fusion begins. Affected cell populations in embryos having 6-26 somite pairs (up to the stage of anterior neuropore closure) were in discrete locations in the alar and basal plates of the rhombencephalon, in the otic placode/vesicle, and in the regions of the epibranchial placodes, olfactory placodes and trigeminal ganglion. The potential basis for the vulnerability of these cell populations to ethanol-induced cell death is discussed. Our understanding of the scope of ethanol-induced CNS damage is dependent upon further defining ethanol-sensitive cell populations at all stages of CNS development.
New small molecule immunosuppressants for transplantation: review of essential concepts. (1994)

More immunosuppressive drugs than ever have recently graduated from the laboratory to extensive clinical trials of their safety and efficacy in patients undergoing transplantation. Although none of these drugs is perfect, they control different forms of rejection in stringent animal models more effectively than other immunosuppressants; yet these novel molecules suppress the immune system far more specifically than steroids and regimens that cause lymphopenia. Cyclosporin G and IMM 125 (analogues of cyclosporine) and FK506 are the only drugs that selectively inhibit T-cell proliferation by blocking cytokine synthesis. The primary action of rapamycin and leflunomide appears to be an inhibition of the actions of cytokines and growth factors on T, B, and some nonimmune cells. T and B cells are more sensitive than nonimmune cells to the depletion of purines and pyrimidines caused by mizoribine, mycophenolic acid, and brequinar sodium. Nucleotide depletion causes interruption of DNA synthesis and glycosylation of adhesion molecules in immune cells. Further differentiation of T and B cells after proliferation into fully functional immune cells is inhibited by unknown mechanisms by brequinar and deoxyspergualin. On the basis of preclinical studies, these drugs may effectively suppress clinical rejection that is (1) acute (all drugs), (2) chronic (rapamycin, leflunomide, and mycophenolic acid), or (3) antibody-mediated (brequinar, deoxyspergualin, mycophenolic acid, and rapamycin). Some drugs (FK506, deoxyspergualin, mycophenolic acid, rapamycin, and leflunomide) may reverse acute rejection refractory to conventional immunosuppression. These new drugs not only block different biochemical steps that normally lead to fully functional T and B cells after stimulation by alloantigen, but their toxicity profiles also differ. Results from preclinical studies predict that use of selected combinations of these drugs in patients will be more effective, less nephrotoxic, less myelotoxic, and less broadly immunosuppressive than current regimens based on cyclosporine, T-cell depletion, steroids, and azathioprine ... at least, that's the idea! Or as former Vice President Dan Quayle said, "It's a question of whether we're going to go forward into the future, or past to the back."
ChemInform Abstract: Synthesis and Reactions of Pyrazolo(3,4‐c)quinoline Derivatives. (1988)

Abstract The title compounds (V) are synthesized as shown.
Andromeda: A Peptide Search Engine Integrated into the MaxQuant Environment (2011)

A key step in mass spectrometry (MS)-based proteomics is the identification of peptides in sequence databases by their fragmentation spectra. Here we describe Andromeda, a novel peptide search engine using a probabilistic scoring model. On proteome data, Andromeda performs as well as Mascot, a widely used commercial search engine, as judged by sensitivity and specificity analysis based on target decoy searches. Furthermore, it can handle data with arbitrarily high fragment mass accuracy, is able to assign and score complex patterns of post-translational modifications, such as highly phosphorylated peptides, and accommodates extremely large databases. The algorithms of Andromeda are provided. Andromeda can function independently or as an integrated search engine of the widely used MaxQuant computational proteomics platform and both are freely available at www.maxquant.org. The combination enables analysis of large data sets in a simple analysis workflow on a desktop computer. For searching individual spectra Andromeda is also accessible via a web server. We demonstrate the flexibility of the system by implementing the capability to identify cofragmented peptides, significantly improving the total number of identified peptides.
Magnetic flux amplification by Lenz lenses (2013)

Tailoring magnetic flux distribution is highly desirable in a wide range of applications such as magnetic sensors and biomedicine. In this paper we study the manipulation of induced currents in passive devices in order to engineer the distribution of magnetic flux intensity in a given region. We propose two different approaches, one based on especially designed wire loops (Lenz law) and the other based on solid conductive pieces (eddy currents). The gain of such devices is mainly determined by geometry giving perspective of high amplification. We consistently modeled, simulated, and executed the proposed devices. Doubled magnetic flux intensity is demonstrated experimentally for a moderate aspect ratio.
Thin endometrium in assisted reproductive technology (2008)

Various recent modalities proposed for the treatment of thin endometrium seem to be useless and inefficient from an evidence-based medicine point of view. At the moment, evaluation of endometrium using different ultrasonographic markers seems to be superior to all those therapies.
A Radio-Over-Fiber Downstream Link Employing Carrier-Suppressed Modulation Scheme to Regenerate and Transmit Vector Signals (2007)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> In this letter, the optical carrier-suppressed (OCS) modulation scheme is employed to transmit vector signals in radio-over-fiber systems. The proposed architecture employing the OCS modulation scheme needs only a balanced single driven Mach–Zehnder optical modulator, and has advantages of dispersion immunity and doubled frequency. Both detailed theoretical analysis and experiments are presented to demonstrate the feasibility of the proposed architecture. </para>