gebhart/patrepeval huggingface dataset:
Adipogenic Differentiation Is Impaired in Replicative Senescent Human Subcutaneous Adipose-Derived Stromal/Progenitor Cells (2013)

We demonstrate that adipose-derived stromal/progenitor cells isolated from abdominal subcutaneous fat pads of adult donors successively enter replicative senescence after long-term cultivation. This is characterized by enlarged cell size, flattened morphology, and upregulated senescence-associated β-galactosidase activity. Moreover, the senescence- associated cyclin-dependent kinase inhibitors p16(Ink4A) and p21(Cip1) were induced correlating with activation of the G1/S cell cycle inhibitor retinoblastoma protein and terminal proliferation arrest. The number of cells in the adipose-derived stromal/progenitor cell population with high adipogenic capacity declined inversely with the increase of senescent cells. Adipogenic hormone cocktail induced expression of the adipogenic key regulators peroxisome proliferator-activated receptor-γ2 and CCAAT/enhancer-binding protein α was significantly reduced in senescent adipose-derived stromal/ progenitor cells. Furthermore, the expression of the adipogenic differentiation genes fatty acid binding protein-4, adiponectin, and leptin and the formation of fat droplets were impaired. We conclude cellular senescence contributes to dysfunctions in adipose-derived stromal/progenitor cell replication, adipogenesis, triglyceride storage, and adipokine secretion.
Successful treatment of intracranial gliomas in rat by oligodeoxynucleotides containing CpG motifs. (2000)

Phosphorothioate oligodeoxynucleotides with CpG motifs (CpG-ODNs) activate various immune cell subsets and induce production of numerous cytokines. To evaluate whether CpG-ODNs can induce rejection of established tumors, Lewis rats were inoculated intracerebrally with syngeneic CNS-1 glioma cells and subsequently injected with CpG-ODNs into the tumor bed. Although all of the control rats (n = 14) died within 23 days, 88% of the animals (n = 8) treated with a single CpG-ODN injection 5 days after tumor inoculation showed long-term survival (>90 days; P < 0.002). CpG-ODNs increased tumoral infiltration with macrophage/microglial cells, CD8, and natural killer lymphocytes. CpG-ODN-cured animals were further protected against a second tumor challenge. CpG-ODNs had no effect on a s.c. CNS1 tumor in nude mice, which suggested that CpG-ODN is not directly cytotoxic and that immunostimulation is required for the antitumoral effect. These findings suggest that intratumoral injections of CpG-ODNs represent a new immunotherapeutic approach in human gliomas, which overcome the need for the selection and purification of a tumoral antigen.
Laboratory Diagnosis of Visceral Leishmaniasis (2002)

The group of diseases known as the leishmaniases are caused by obligate intracellular protozoa of the genus Leishmania ([39][1]). Natural transmission of leishmania is carried out by a certain species of sandfly of the genus Phlebotomus (Old World) or Lutzomyia (New World). These are present in
Integrin-Associated Protein Stimulates α2β1-Dependent Chemotaxis via GI-Mediated Inhibition of Adenylate Cyclase and Extracellular-Regulated Kinases (1999)

Integrin-associated protein (IAP/CD47) augments the function of alpha2beta1 integrin in smooth muscle cells (SMC), resulting in enhanced chemotaxis toward soluble collagen (Wang, X-Q., and W.A. Frazier. 1998. Mol. Biol. Cell. 9:865). IAP-deficient SMC derived from IAP(-/-) animals did not migrate in response to 4N1K (KRFYVVMWKK), a peptide agonist of IAP derived from the COOH-terminal domain of thrombospondin-1 (TSP1). When normal SMC were preincubated with 4N1K or an anti-alpha2beta1 function-stimulating antibody, cell migration to soluble collagen was significantly enhanced. 4N1K-induced chemotaxis was blocked by treatment of SMC with pertussis toxin indicating that IAP acts through Gi. In agreement with this, 4N1K evoked a rapid decrease in cAMP levels which was intensified in the presence of collagen, and forskolin and 8-Br-cAMP both inhibited SMC migration stimulated via IAP. 4N1K strongly inhibited extracellular regulated kinase (ERK) activation in SMC attaching to collagen and reduced basal ERK activity in suspended SMC. Pertussis toxin treatment of SMC significantly activated ERK, suggesting that an inhibitory input was alleviated. Inhibition of ERK activity by (a) the MAP kinase kinase (MEK) inhibitor, PD98059, (b) antisense oligonucleotide depletion of ERK, and (c) expression of mitogen-activated protein (MAP) kinase phosphatase-1 in SMC all led to increased migration to collagen, 4N1K, or 4N1K plus collagen. Thus, IAP stimulates alpha2beta1 integrin-mediated SMC migration via Gi-mediated inhibition of ERK activity and suppression of cyclic AMP levels. Both of these signaling pathways could directly modulate the state of the integrin as well as impact downstream components of the cell motility apparatus.
A New Bone Marrow Transplantation Method for the Prevention of Graft- Versus-Host Disease (2005)

A new bone marrow transplantation (BMT) method, "intra-bone marrow (IBM)-BMT" has recently been developed. This method was found to prevent not only graft-versus-host (GvH) reaction but also host-versus-graft (HvG) reaction, since IBM-BMT can efficiently recruit donor-derived stromal cells (including mesenchymal stem cells: MSCs), which produce immunosuppressive cytokines. This paper shows that IBM-BMT prevents GvHD even when donor lymphocyte infusion (DLI) is carried out, and that the combination of IBM-BMT + DLI not only prevents GvHD but also inhibits the growth of solid tumors in mice. In addition, it has been shown that IBM-BMT will be applicable to the treatment of various intractable diseases. Keywords: Bone marrow transplantation, graft-versus-host reaction, host-versus-graft reaction, donor lymphocyte infusion, graft-versus-tumor reaction
Theoretical analysis of subwavelength high contrast grating reflectors (2010)

A simple analytic analysis of the ultra-high reflectivity feature of subwavelength dielectric gratings is developed. The phenomenon of ultra high reflectivity is explained to be a destructive interference effect between the two grating modes. Based on this phenomenon, a design algorithm for broadband grating mirrors is suggested.
Electrophysiological response patterns of primary sensory cortices in migraine (2006)

Migraine is an ictal disorder characterised by a particular vulnerability of patients to sensory overload, both during and outside of the attack. Central nervous system dysfunctions are supposed to play a pivotal role in migraine. Electroneurophysiological methods, which aim to investigate sensory processing, seem thus particularly appropriate to study the pathophysiology of migraine. We have thus reviewed evoked potential studies performed in migraine patients. Although results are in part contradictory, these studies nonetheless demonstrate an interictal dysfunction of sensory cortices, and possibly of subcortical structures, in migraine with and without aura. The predominant abnormality is a deficient habituation of evoked responses to repeated stimuli, probably due to cortical, and possibly widespread neural, "dysexcitability".
Ultracentrifuge studies of the binding of IgG of different subclasses to the Clq subunit of the first component of complement (1976)

Normal IgG and myeloma proteins of the IgG1, 2, 3,and 4 subclasses were mixed with human Clq and studied in the analytical ultracentrifuge for complex formation. Binding of IgG to Clq is apparent both from the enlargement of area and from the increase in sedimentation rate of the well-separated schlieren peak of Clq. The accurate determination of binding parameters requires that sedimentation rates be corrected for hydrodynamic interaction, and area measurements corrected for the Johnston-Ogston effect. At the highest immunoglobulin concentrations employed in these studies more than ten IgG molecules are bound to each Clq. If we assume that the number of binding sites must be an integral multiple of 6, then the data best support a 12 binding site model, although an 18 site model cannot be rule out. Myeloma IgG proteins of all subclasses bind to Clq, with affinities decreasing in the order G3 greater than G1 greater than G2 greater than G4. No binding of IgA to Clq could be detected.
Enhancement of expression of exogenous genes by 2-aminopurine. Regulation at the post-transcriptional level. (1991)

In human and mouse cell lines, expression of exogenous genes was enhanced by treatment with 2-aminopurine (2-AP). Chloramphenicol acetyltransferase (CAT) and neomycin phosphotransferase activities were increased by up to 50-fold upon 2-AP treatment of cells permanently transfected with genes encoding these enzymes. Neomycin phosphotransferase activity was also increased by this treatment in cells infected with a retroviral vector carrying the neomycin phosphotransferase gene. 2-AP-mediated increase in CAT activity was observed in various cell lines which had been permanently transfected with chimeric CAT genes containing transcriptional regulatory elements of the interferon-inducible genes 6-16 and 561, SV40 early genes, mouse mammary tumor viral gene, or metallothionein II gene. The increase in the cellular CAT enzymatic activity was due to an elevated level of CAT protein. The 2-AP-mediated enhancement of CAT expression was operative at the translational level; the rate of transcription of CAT mRNA or its steady-state level was affected only marginally. The translational up-regulation by 2-AP was restricted to the genes introduced from outside; there was no gross change in the rate of synthesis of other cellular proteins.
Blockade of interleukin-6 signaling augments regulatory T-cell reconstitution and attenuates the severity of graft-versus-host disease (2009)

Graft-versus-host disease (GVHD) is the major complication after allogeneic bone marrow transplantation and is characterized by the overproduction of proinflammatory cytokines. In this study, we have identified interleukin-6 (IL-6) as a critical inflammatory cytokine that alters the balance between the effector and regulatory arms of the immune system and drives a proinflammatory phenotype that is a defining characteristic of GVHD. Our results demonstrate that inhibition of the IL-6 signaling pathway by way of antibody-mediated blockade of the IL-6 receptor (IL-6R) markedly reduces pathologic damage attributable to GVHD. This is accompanied by a significant increase in the absolute number of regulatory T cells (Tregs) that is due to augmentation of thymic-dependent and thymic-independent Treg production. Correspondingly, there is a significant reduction in the number of T helper 1 and T helper 17 cells in GVHD target organs, demonstrating that blockade of IL-6 signaling decreases the ratio of proinflammatory T cells to Tregs. These studies demonstrate that antibody blockade of the IL-6R serves to recalibrate the effector and regulatory arms of the immune system and represents a novel, potentially clinically translatable, strategy for the attenuation of GVHD.
Overview and Principles of Internet Traffic Engineering (2002)

This memo describes the principles of Traffic Engineering (TE) in the Internet. The document is intended to promote better understanding of the issues surrounding traffic engineering in IP networks, and to provide a common basis for the development of traffic engineering capabilities for the Internet. The principles, architectures, and methodologies for performance evaluation and performance optimization of operational IP networks are discussed throughout this document.
Characterization of the melt removal rate in laser cutting of thick-section stainless steel (2010)

The efficiency of the laser cutting process depends on both the rate of melting and rate of melt removal from the cut kerf. The depth of flow separation and the dross attachment on the lower cut edge relate to the efficiency of the melt removal process and can be used to characterize the rate of melt removal from the cut kerf. The melt flow velocity and melt film thickness are formulated in this study by consideration of the fundamentals of viscous incompressible fluid flow. The calculated melt flow velocity and melt film thickness are correlated with the depth of flow separation on the 10 mm stainless steel AISI 304 (EN 1.4301) laser cut edge. The effects of process parameters—including assist gas pressure, nozzle diameter, nozzle standoff, focal point position, and cutting speed—on the depth of flow separation and the dross attachment on the lower cut edge are investigated. The assist gas pressure, nozzle diameter, and focal point position are found to significantly affect the efficiency of melt removal from the cut kerf.
Some Potential Errors in the Identification of Hail Swaths and Seeded Storms (1996)

In many hail supression projects currently under development in western Europe, ground generator networks are deployed. The plumes of AgI emitted by these, containing hgh concentrations of ice nuclei, follow the wind trajectory. When an evaluation of the Project needs to be made, it is essential to be able to determine whether storms which gave rise to hailfalls were seeded or not, and to establish the exact extent of the zones affected. In this study, a discussion will be made of the criteria which need to be applied in order to avoid errors in the evaluation of seeded and non-seeded thunderstorm samples.
FEMALES TOLERATE THE DELETERIOUS CONSEQUENCES OF HEMORRHAGE AND SUBSEQUENT SEPSIS BETTER THAN MALES (1999)

Diodato, M. D.; Knöferl, M. W.; Angele, M. K.; Schwacha, M. G.; Cioffi, W. G.; Bland, K. I.; Chaudry, I. H. Author Information
Physical linkage of the genes for platelet membrane glycoproteins IIb and IIIa. (1988)

The fibrinogen receptor on human platelets is a prototypic member of the integrin family and is composed of subunit glycoproteins IIb (gpIIb) and IIIa (gpIIIa) in a 1:1 stoichiometric ratio. We have isolated cDNA clones for gpIIb and gpIIIa and localized both genes to chromosome 17. In the current study, several approaches were used to localize and map the genes for gpIIb and gpIIIa. A preliminary evaluation of subchromosomal localization was performed by using a panel of mouse-human somatic cell hybrids that contain different amounts of the long arm of human chromosome 17. Southern hybridization to the DNA of these hybrids shows that both genes map near the thymidine kinase gene. In situ hybridization to intact human chromosomes localized both genes to the 17q21-22 region. To better define the physical distance between the two genes, we examined the genomic hybridization pattern of each cDNA probe to high molecular weight restriction fragments separated by pulsed-field gel electrophoresis. Serial hybridizations of the same filter have allowed construction of long-range Mlu I and Sfi I restriction maps spanning more than 500 kilobases. Finally, nonoverlapping portions of the cDNAs for both gpIIb and gpIIIa were used to probe Sfi I digests of genomic DNA separated by field-inversion gels. This confirmed that the genes are physically linked within the same 260-kilobase Sfi I fragment and suggests that the gene for gpIIb is located on the 3' side of the gene for gpIIIa. These results suggest that coordinate expression of gpIIb and gpIIIa may depend on physical proximity.
Matrix-Mediated Synthesis of Nanocrystalline γ-Fe2O3: A New Optically Transparent Magnetic Material (1992)

A new magnetic material with appreciable optical transmission in the visible region at room temperature has been isolated as a gamma-Fe(2)O(3)/polymer nanocomposite. The synthesis is carried out in an ion-exchange resin at 60 degrees C. Magnetization and susceptibility data demonstrate loading-dependent saturation moments as high as 46 electromagnetic units per gram and superparamagnetism for lower loadings where particle sizes are less than 100 angstroms. Optical absorption studies show that the small-particle form of gamma-Fe(2)O(3) is considerably more transparent to visible light than the single-crystal form. The difference in absorption ranges from nearly an order of magnitude in the "red" spectral region to a factor of 3 at 5400 angstroms. The magnetization of the nanocomposite is greater by more than an order of magnitude than those of the strongest room-temperature transparent magnets, FeBO(3) and FeF(3).
Cover Picture: Green Microwave Synthesis of MIL‐100(Al, Cr, Fe) Nanoparticles for Thin‐Film Elaboration (Eur. J. Inorg. Chem. 32/2012) (2012)

The front cover picture shows nanoparticles of mesoporous metal trimesate MIL-100(Al, Cr or Fe) metal–organic framework, which were synthesized by a microwave-assisted solvothermal route using green solvents. The optimization of yield, crystallinity, and particle size was achieved through the control of synthetic parameters. The particle size strongly depends on the nature of the metallic cation and the organic precursor, leading to nanoparticles smaller than 100 nm for iron- and chromium-based MIL-100 . Finally, stable colloidal solutions of iron and chromium MIL-100 nanoparticles were obtained to prepare thin films of high optical quality by dip coating, and their porosity was investigated by environmental ellipsometry. Details are discussed in the article by P. Horcajada, C. Serre, C. Boissière et al. on p. 5165 ff.
Electrochemical Insertion/extraction of Lithium in Multiwall Carbon Nanotube/Sb and SnSb₀.₅ Nanocomposites (2003)

Multiwall carbon nanotubes (CNTs) were synthesized by catalytic chemical vapor deposition of acetylene and used as templates to prepare CNT-Sb and CNT-SnSb₀.₅ nanocomposites via the chemical reduction of SnCl₂ and SbCl₃ precursors. SEM and TEM imagings show that the Sb and SnSb₀.₅ particles were uniformly dispersed in the CNT web and on the outside surface of CNTs. These CNT-metal composites are active anode materials for lithium ion batteries, showing improved cyclability compared to unsupported Sb and SnSb particles; and higher reversible specific capacities than CNTs. The improvement in cyclability may be attributed to the nanoscale dimensions of the metal particles and CNT’s role as a buffer in containing the mechanical stress arising from the volume changes in electrochemical lithium insertion and extraction reactions.
Transfected Cyclic Nucleotide–Gated Channels as Biosensors (2000)

The cyclic nucleotides, cyclic AMP and cyclic GMP, like intracellular calcium, are involved in a vast array of intracellular signaling processes. But knowledge of the cellular and subcellular concentrations of cAMP and cGMP, and of the kinetics and spatial distributions of the changes of their concentration in situ, have lagged far behind our knowledge of the corresponding features of calcium signaling, which were propelled by developments in calcium-sensing dyes and imaging technology (Tsien 1989, Tsien 1998). In this issue, J.W. Karpen and colleagues advance the issue of cAMP measurement in living cells by developing and convincingly calibrating the use of transfected cyclic nucleotide–gated channels as cAMP sensors (Rich et al. 2000).
Distinct IL-2 Receptor Signaling Pattern in CD4+CD25+ Regulatory T Cells (2004)

Despite expression of the high-affinity IL-2R, CD4(+)CD25(+) regulatory T cells (Tregs) are hypoproliferative upon IL-2R stimulation in vitro. However the mechanisms by which CD4(+)CD25(+) T cells respond to IL-2 signals are undefined. In this report, we examine the cellular and molecular responses of CD4(+)CD25(+) Tregs to IL-2. IL-2R stimulation results in a G(1) cell cycle arrest, cellular enlargement and increased cellular survival of CD4(+)CD25(+) T cells. We find a distinct pattern of IL-2R signaling in which the Janus kinase/STAT pathway remains intact, whereas IL-2 does not activate downstream targets of phosphatidylinositol 3-kinase. Negative regulation of phosphatidylinositol 3-kinase signaling and IL-2-mediated proliferation of CD4(+)CD25(+) T cells is inversely associated with expression of the phosphatase and tensin homologue deleted on chromosome 10, PTEN.
Continuous, Noninvasive Measurement of Pulsus Paradoxus in Patients With Acute Asthma (1996)

Our measurement technique for PP is a useful objective indicator of the severity of asthma. Pulsus paradoxus correlates with clinical score, peak expiratory flow rate, and oxygen saturation. Furthermore, an elevated PP is associated with the decision to admit a patient for further treatment. These results suggest that this technique may represent a useful research tool for the assessment of acute, severe asthma. Further development of the technology may allow for a clinical application.
Strategies for handover and dynamic channel allocation in micro-cellular mobile radio systems (2003)

Microcellular structures are the central means for increasing the network capacity of mobile radio systems. By examining existing dynamic channel allocation algorithms, the authors identify a class of adaptive algorithms best suited for such microcellular environments. Applicable algorithms of this class require intracell handover and are to a large extent decentralized. One new algorithm of this class is introduced and evaluated by means of simulation. The proposed algorithm (DYNINF) has been evaluated with respect to performance criteria such as blocking probability, interference probability, and number of handovers (stability). It is shown that, based on the capacity improvement achieved by microcells, no relevant further improvement of the capacity can be realized, but that the algorithm is very well suited to cope with the problems of microcells, e.g. discrepancies between the network plan and the real situation and heavy variations of the traffic and interference conditions.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Ipsilon Flow Management Protocol Specification for IPv4 Version 1.0 (1996)

The Ipsilon Flow Management Protocol (IFMP), is a protocol for allowing a node to instruct an adjacent node to attach a layer 2 label to a specified IP flow. The label allows more efficient access to cached routing information for that flow. The label can also enable a node to switch further packets belonging to the specified flow at layer 2 rather than forwarding them at layer 3.