gebhart/patrepeval huggingface dataset:
Vitamin A as an enzyme that catalyzes the reduction of MTT to formazan by vitamin C (2000)

The tetrazolium salt 3(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) is reduced to formazan by the succinate dehydrogenase system of active mitochondria, and hence, specifically used to assay for the viable cells, such as measurement of cell proliferation, cytotoxicity, and cell number. However, in the present study we have shown that some component specifically present in M199 but not in RPMI 1640 media can reduce MTT to formazan in the absence of a living system. Further study revealed that ascorbic acid reduced MTT to formazan, which was profoundly increased by a very small amount of retinol, whereas retinol alone had no effect. Oxidation of ascorbic acid by H(2)O(2) destroyed its ability to reduce MTT. The rate of MTT reduction was directly proportional to the concentration of MTT in the absence of retinol, but approached a zero-order state beyond a certain concentration of MTT in the presence of retinol. Furthermore, retinol remained unchanged after the completion of the reaction. Taken together, these results showed that retinol acts as a reductase that catalyzes the reduction of MTT to formazan using ascorbic acid as the cosubstrate (electron donor).
Nanoscale Particles Removal on an Extreme Ultra-Violet Lithography (EUVL) Mask Layer (2006)

Abstract not Available.
Agonist- and voltage-gated calcium entry in cultured mouse spinal cord neurons under voltage clamp measured using arsenazo III (1987)

Spinal cord neurons is dissociated cell culture were loaded with the calcium indicator arsenazo III using the whole-cell patch-clamp recording technique. Under voltage-clamp, depolarizing voltage steps evoked transient increases in absorbance at 660 nm, with no change at 570 nm, the isosbestic wavelength for calcium-arsenazo III complexes. The optical response occurred with a threshold depolarization to -30 mV, peaked at +10 mV, and decreased with further depolarization, consistent with an elevation of cytoplasmic free calcium resulting from Ca2+ flux through voltage-dependent calcium channels. Inward current responses to the excitatory amino acids N-methyl-D-aspartic acid (NMDA) and L-glutamate were also accompanied by calcium transients; these were dose-dependent, varied with the driving force for inward current, and were blocked by extracellular Mg2+ in a voltage-dependent manner, suggesting Ca2+ flux through NMDA-receptor channels. Responses to kainate, quisqualate, and GABA were not accompanied by comparable calcium transients. [Ca2+]i transients evoked by depolarizing voltage steps were of maximal amplitude at the start of recording and declined with time, reflecting rundown of voltage-dependent calcium channels. In contrast, [Ca2+]i transients evoked by NMDA gradually increased in amplitude during periods of whole-cell recording lasting 1-2 hr. Procedures resulting in loading of the neuron with Ca2+ accelerated the increase in amplitude of [Ca2+]i transients evoked by NMDA, but slowed the decay of [Ca2+]i transients evoked by voltage steps. Our results provide evidence for 2 independent sources of transmembrane Ca2+ flux in vertebrate neurons, through voltage-gated calcium channels and through NMDA-receptor channels. The Ca2+ flux gated by NMDA-receptor-specific agonists may play a role in synaptic plasticity, in regulating excitability, and in the excitotoxic response to excitatory amino acids.
Phase I Study of ARN-509, a Novel Antiandrogen, in the Treatment of Castration-Resistant Prostate Cancer (2013)

ARN-509 was safe and well tolerated, displayed dose-proportional pharmacokinetics, and demonstrated pharmacodynamic and antitumor activity across all dose levels tested. A maximum efficacious dose of 240 mg daily was selected for phase II exploration based on integration of preclinical and clinical data.
Evidence That the Rab3a-binding Protein, Rabphilin3a, Enhances Regulated Secretion. (1995)

Rabphilin3a had been identified in brain as a Rab3a-binding protein and may serve as an effector for Rab3a function. We have cloned a splice variant of brain-Rabphilin3a from a bovine adrenal chromaffin cell cDNA library and investigated the function of the protein in regulated exocytosis in bovine chromaffin cells. The predicted amino acid sequence of chromaffin cell (c-) Rabphilin3a was identical with that of brain (b-) Rabphilin3a except for a 6-amino-acid insert VFSLSA in the amino-terminal half of the protein. An antibody directed against a carboxyl-terminal peptide recognized an 85-kDa protein in COS7 cells transfected with the cDNA in a mammalian expression vector. A band of similar mobility was enriched in a fraction of highly purified chromaffin granule membranes, consistent with the Rabphilin3a being associated with chromaffin granule membranes. Overexpression of either chromaffin cell or brain Rabphilin3a by transfection with the corresponding cDNAs in mammalian expression vectors enhanced DMPP-induced secretion of co-expressed human growth hormone (GH) approximately 30%. Chromaffin cells transfected with a plasmid with the entire coding sequence of c-Rabphilin3a inserted in the antisense orientation inhibited secretion of co-expressed GH by approximately 30%. Rabphilin3a mutants lacking one or both of the carboxyl-terminal C2 domains strongly inhibited DMPP-stimulated exocytosis. The single C2 domain deletion also strongly inhibited Ca(2+)-dependent secretion from digitonin-permeabilized cells. These data indicate that Rabphilin3a is a positive regulator of exocytosis. Because the C2 deletion mutants contain the amino-terminal Rab3a-GTP binding domain, they may inhibit secretion by competing with endogenous Rabphilin3a for interaction with Rab3a-GTP without being able to mimic the functional effects of full-length Rabphilin3a.
Monolithic Silicon Photonics at 25 Gb/s (2016)

Monolithic CMOS photonics seeks to minimize total transceiver cost by simplifying packaging, design and test. Here we examine 25 Gb/s applications in the context of integrated transistor performance and demonstrate a 4λ×25 Gb/s reference design.
Characterization and acidic properties of silica pillared titanates (2001)

Reaction of alkylammonium titanates with tetraethylorthosilicate (TEOS) resulted in the intercalation and polymerization of TEOS between the titanate sheets. After heating to 500 °C, the basal spacing of pillared silica titanates increased with increasing length of the alkylamine chains and the amount of silicon intercalated. Although the layers of pillared titanates were expanded by the silica species, and the specific surface area increased, only negligible micropores were formed, suggesting that the interlayer region was “stuffed” with the pillaring species. The adsorption of pyridine indicated the formation of strong Brönsted acid sites up to 300 °C. In addition some basic Lewis sites were detected on silica pillared titanates.
Hydrolysis of Vegetable Oils in Sub- and Supercritical Water (1997)

Water, in its subcritical state, can be used as both a solvent and reactant for the hydrolysis of triglycerides. In this study, soybean, linseed, and coconut oils were successfully and reproducibly hydrolyzed to free fatty acids with water at a density of 0.7 g/mL and temperatures of 260−280 °C. Under these conditions the reaction proceeds quickly, with conversion of greater than 97% after 15−20 min. Some geometric isomerization of the linolenic acids was observed at reaction temperatures as low as 250 °C. Reactions carried out at higher temperatures and pressures, up to the critical point of water, produced either/or degradation, pyrolysis, and polymerization, of the oils and resultant fatty acids.
Depletion of Murine CD4+ T Lymphocytes Prevents Antigen-Induced Airway Hyperreactivity and Pulmonary Eosinophilia (1994)

Abstract The pathogenesis of asthma remains unclear. An in vivo murine model of antigen-induced airway hyperreactivity and inflammation was developed to investigate the possibility, suggested by a wealth of descriptive human data, that alterations in immunoregulation are important in the genesis of airway hyperreactivity. A/J mice developed airway hyperreactivity and markedly increased numbers of pulmonary inflammatory cells following intraperitoneal sensitization and intratracheal challenge with sheep red blood cells. Notably, eosinophils were a prominent component of the inflammatory infiltrate. The dependence of these phenomena, both pathologic and functional, on CD4+ T lymphocytes was investigated by in vivo depletion of CD4+ cells using the anti-CD4 mAb GK1.5. When administered before antigen challenge, GK1.5 completely prevented both airway hyperreactivity and the infiltration of eosinophils. This model provides the first direct demonstration of the dependence of airway hyperreactivity upon CD4+ T lymphocytes, and the results are consistent with the possibility that eosinophils are effectors of this response.
Noninvasive Detection of HER2 Amplification with Plasma DNA Digital PCR (2013)

Analysis of plasma DNA with digital PCR has the potential to screen for the acquisition of HER2 amplification in metastatic breast cancer. This approach could potentially be adapted to the analysis of any locus amplified in cancer.
Prolonged Reactive Oxygen Species Generation and Nuclear Factor-κB Activation after a High-Fat, High-Carbohydrate Meal in the Obese (2007)

High-fat, high-carbohydrate meals induced a significantly more prolonged and greater oxidative and inflammatory stress in the obese. This may contribute to the increased atherogenic risk in obesity.
Targeting Microtubules by Natural Agents for Cancer Therapy (2014)

Natural compounds that target microtubules and disrupt the normal function of the mitotic spindle have proven to be one of the best classes of cancer chemotherapeutic drugs available in clinics to date. There is increasing evidence showing that even minor alteration of microtubule dynamics can engage the spindle checkpoint, arresting cell-cycle progression at mitosis and subsequently leading to cell death. Our improved understanding of tumor biology and our continued appreciation for what the microtubule targeting agents (MTAs) can do have helped pave the way for a new era in the treatment of cancer. The effectiveness of these agents for cancer therapy has been impaired, however, by various side effects and drug resistance. Several new MTAs have shown potent activity against the proliferation of various cancer cells, including resistance to the existing MTAs. Sustained investigation of the mechanisms of action of MTAs, development and discovery of new drugs, and exploring new treatment strategies that reduce side effects and circumvent drug resistance could provide more effective therapeutic options for patients with cancer. This review focuses on the successful cancer chemotherapy from natural compounds in clinical settings and the challenges that may abort their usefulness.
The Atypical Protein Kinase C-interacting Protein p62 Is a Scaffold for NF-κB Activation by Nerve Growth Factor (2001)

Nerve growth factor (NGF) binding to both p75 and TrkA neurotrophin receptors activates the transcription factor nuclear factor kappaB (NF-kappaB). Here we show that the atypical protein kinase C-interacting protein, p62, which binds TRAF6, selectively interacts with TrkA but not p75. In contrast, TRAF6 interacts with p75 but not TrkA. We demonstrate the formation of a TRAF6-p62 complex that serves as a bridge linking both p75 and TrkA signaling. Of functional relevance, transfection of antisense p62-enhanced p75-mediated cell death and diminished NGF-induced differentiation occur through a mechanism involving inhibition of IKK activity. These findings reveal a new function for p62 as a common platform for communication of both p75-TRAF6 and TrkA signals. Moreover, we demonstrated that p62 serves as a scaffold for activation of the NF-kappaB pathway, which mediates NGF survival and differentiation responses.
Prehistory of the Notion of Circulating Nucleic Acids in Plasma/Serum (CNAPS) (2006)

In the late 50s and early 60s of the last century, a theoretical fight was taking place between Western and Russian scientists about the theory explaining the mechanism of evolution. According to neo-Darwinism, evolution was the result of hazard and necessity, that is, mutations arriving by chance favoring the survival of the fittest. For the Russian geneticists, acquired characteristics were the basis of evolution, that is, the environment modified the characteristics of the gene. One of the main experiments on which the Russian geneticists based their theory was the transmission of hereditary characteristics by a special technique of grafting between two varieties of plants-a mentor plant and a pupil plant. The pupil variety being entirely dependent on the development of the mentor plant its hereditary characteristics were modified accordingly. In the Western world these experiments were regarded with doubt. We were among the few who tried to repeat this kind of experiment. After three generations of grafting between two varieties of eggplant, we succeeded in obtaining hereditary modifications of the pupil plants, which acquired some of the characteristics of the mentor variety. The linkage between some hereditary characteristics of the mentor plant were broken, the segregation of the offspring was abnormal, dominant characteristics appearing in the offspring of a recessive plant. Rather than adopting the views of the Russian scientists about acquired characteristics, we suggested that DNA was circulating between the mentor and pupil plants and assumed that some nucleic acid molecules bearing genetic information could enter the somatic and reproductive cells of the pupil plant at a propitious moment and remain active.
Sodium-taurocholate-induced acute necrotizing pancreatitis does not affect jejunal oxygenation in pigs (1994)

Our results demonstrate that, under conditions of sustained systemic hemodynamics, jejunal oxygen transport and mucosal oxygenation are well maintained during the early course of sodium-taurocholate-induced acute necrotizing pancreatitis.
Numerical determination of hysteresis parameters for the modeling of magnetic properties using the theory of ferromagnetic hysteresis (1992)

The authors describe how the various model parameters needed to describe hysteresis on the basis of the Jiles-Atherton theory can be calculated from experimental measurements of the coercivity, remanence, saturation magnetization, initial anhysteretic susceptibility, initial normal susceptibility, and maximum differential susceptibility. The determination of hysteresis parameters based on this limited set of magnetic properties is of the most practical use since these are the properties of magnetic materials that are most likely to be available.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Local Utility Power Line Communications in the 3–500 kHz Band: Channel Impairments, Noise, and Standards (2012)

Future smart grid systems will intelligently monitor and control energy flows to improve the efficiency and reliability of power delivery. This monitoring and control requires low-delay, highly reliable communication between customers, local utilities, and regional utilities. A vital part of future smart grids is the two way communication links between smart meters at the customer sites and a (decentralized) command and control center operated by the local utility. To enable these two-way communication links, narrowband power line communication (PLC) systems operating in the 3-500 kHz band are attractive because they can be deployed over existing outdoor power lines. Power lines, however, have traditionally been designed for one-directional power delivery and remain hostile environments for communication signal propagation. In this article, we review signal processing approaches to model channel impairments and impulsive noise and mitigate their effects in narrowband PLC systems. We examine ways to improve the communication performance based on current and emerging standards.
Methimazole (2-Mercapto 1-Methyl Imidazole) in Psoriasis – Results of an Open Trial (1993)

Methimazole, an antithyroid drug, was orally administered, in an open trial, in a dose of 20 mg every 12 h for 8 weeks to 8 volunteers with long-standing psoriasis. 3-mm punch biopsies were taken from the lesions at the start and at the end of the study. Clinical response was assessed using the Psoriasis Areas Severity Index score. Methimazole produced marked to moderate improvement in the clinical scores in the majority of patients. Histological scores were also significantly improved in all patients. Unexpectedly, thyroid function tests were not affected by methimazole therapy in all but one patient, and none of the patients developed drug-induced cytopenia. Methimazole may be an effective therapeutic agent in the management of psoriasis; it most probably exerts its therapeutic effect by acting on the immune system.
Evaluation of the hepatic NK cell response duringthe early phase of Fasciola hepatica infection in rats (2002)

The in situ distribution of NK cells in rat liver during the first 28 days of an experimental infection with F hepatica was investigated. NK cells were distributed homogeneously throughout the hepatic parenchyma in uninfected animals. The total number of hepatic mononuclear cells increased significantly following infection, but the proportion of NK cells did not change. After infection, these cells were found around the portal space, around the centrolobular vein, in the periportal fibrosis and in the band of collagen. However, no NK cells could be detected in or around the granuloma during infection. The frequency of both I L-2- and IFNgamma-producing NK cells was higher on day 7 postinfection (pi) but only the percentage of IFNgamma -CD161+ subsets remained elevated thereafter, whereas the percentage of both IL-2+CD161+ and IL-4+CD161+ subsets returned to the baseline. The number of CD161+IL10+ cells did not change significantly. These results suggest that NK cells could be another source for the early production of IFNgamma but provide no evidence that these cells are involved in early events associated with granuloma formation.
Palindromic Nucleotide Analysis in Human T Cell Receptor Rearrangements (2012)

Diversity of T cell receptor (TCR) genes is primarily generated by nucleotide insertions upon rearrangement from their germ line-encoded V, D and J segments. Nucleotide insertions at V-D and D-J junctions are random, but some small subsets of these insertions are exceptional, in that one to three base pairs inversely repeat the sequence of the germline DNA. These short complementary palindromic sequences are called P nucleotides. We apply the ImmunoSeq deep-sequencing assay to the third complementarity determining region (CDR3) of the β chain of T cell receptors, and use the resulting data to study P nucleotides in the repertoire of naïve and memory CD8(+) and CD4(+) T cells. We estimate P nucleotide distributions in a cross section of healthy adults and different T cell subtypes. We show that P nucleotide frequency in all T cell subtypes ranges from 1% to 2%, and that the distribution is highly biased with respect to the coding end of the gene segment. Classification of observed palindromic sequences into P nucleotides using a maximum conditional probability model shows that single base P nucleotides are very rare in VDJ recombination; P nucleotides are primarily two bases long. To explore the role of P nucleotides in thymic selection, we compare P nucleotides in productive and non-productive sequences of CD8(+) naïve T cells. The naïve CD8(+) T cell clones with P nucleotides are more highly expanded.
Single-walled carbon nanotube probes for AFM imaging (2004)

This paper discusses fabrication and characterization of single-walled carbon nanotube (SWNT) AFM probes. By selectively deposit catalytic nanoparticles onto commercial AFM probes with silicon tips, we are able to grow SWNTs on the wafer scale, with 30-50% usable probe yield. We also studied shortening of carbon nanotubes to achieve proper mechanical strength required for AFM application. Comparison shows that SWNT probes consistently reveal 50-100% more surface features than silicon probes. These probes are also successfully used in DNA imaging in a liquid solution.
17.7% efficiency large area multicrystalline silicon solar cell using screen-printed metallization technique (2005)

This paper describes the development of an industrial processing sequence that employs the screen printing and firing for large area multicrystalline silicon (mc-Si) solar cells. A record high efficiency mc-Si solar cell of 17.7%; cell area: 232.5 cm/sup 2/ has been achieved using Kyocera's original cast mc-Si material. The diffusion length of mc-Si material was improved after cell processing especially at the region that had low diffusion length by gettering or passivation effects. The diffusion length after cell processing was about 270 /spl mu/m, and this value corresponds to the value estimated internal quantum efficiency curve of PC-1D simulation very well. The photovoltaic module has been fabricated using 48 cells that have average efficiency of 17.2% with this type of cell. The record module efficiency of 15.7% has been achieved.
Structural insights into the inhibited states of the Mer receptor tyrosine kinase (2008)

The mammalian ortholog of the retroviral oncogene v-Eyk, and a receptor tyrosine kinase upstream of antiapoptotic and transforming signals, Mer (MerTK) is a mediator of the phagocytic process, being involved in retinal and immune cell clearance and platelet aggregation. Mer knockout mice are viable and are protected from epinephrine-induced pulmonary thromboembolism and ferric chloride-induced thrombosis. Mer overexpression, on the other hand, is associated with numerous carcinomas. Although Mer adaptor proteins and signaling pathways have been identified, it remains unclear how Mer initiates phagocytosis. When bound to its nucleotide cofactor, the high-resolution structure of Mer shows an autoinhibited alphaC-Glu-out conformation with insertion of an activation loop residue into the active site. Mer complexed with compound-52 (C52: 2-(2-hydroxyethylamino)-6-(3-chloroanilino)-9-isopropylpurine), a ligand identified from a focused library, retains its DFG-Asp-in and alphaC-Glu-out conformation, but acquires other conformational changes. The alphaC helix and DFGL region is closer to the hinge region and the ethanolamine moiety of C52 binds in the groove formed between Leu593 and Val601 of the P-loop, causing a compression of the active site pocket. These conformational states reveal the mechanisms of autoinhibition, the pathophysiological basis of disease-causing mutations, and a platform for the development of chemical probes.
Relationship between haemagglutination-inhibiting antibody titres and clinical protection against influenza: development and application of a bayesian random-effects model (2010)

Although limitations in the data used should not be overlooked, the relationship derived in this analysis provides a means to predict the efficacy of inactivated influenza vaccines when only immunogenicity data are available. This relationship can also be useful for comparing the efficacy of different influenza vaccines based on their immunological profile.
Tuning up the compression and applying the choke for better horsepower in resuscitation* (2006)

Department of Anesthesia and Critical Care, Massachusetts General Hospital, Harvard Medical School, Boston, MA