gebhart/patrepeval huggingface dataset:
Peroxisomes as a source of superoxide and hydrogen peroxide in stressed plants (1996)

Conference Article| May 01 1996 Peroxisomes as a source of superoxide and hydrogen peroxide in stressed plants L. A. del Rio; L. A. del Rio * *To whom correspondence should be addressed Search for other works by this author on: This Site PubMed Google Scholar J. M. Palma; J. M. Palma 1Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apdo. 419, E-18080 Granada, Spain Search for other works by this author on: This Site PubMed Google Scholar L. M. Sandalio; L. M. Sandalio 1Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apdo. 419, E-18080 Granada, Spain Search for other works by this author on: This Site PubMed Google Scholar F. J. Corpas; F. J. Corpas 1Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apdo. 419, E-18080 Granada, Spain Search for other works by this author on: This Site PubMed Google Scholar G. M. Pastori; G. M. Pastori 1Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apdo. 419, E-18080 Granada, Spain Search for other works by this author on: This Site PubMed Google Scholar P. Bueno; P. Bueno 1Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apdo. 419, E-18080 Granada, Spain Search for other works by this author on: This Site PubMed Google Scholar E. López-Huertas E. López-Huertas 1Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apdo. 419, E-18080 Granada, Spain Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1996) 24 (2): 434–438. https://doi.org/10.1042/bst0240434 Article history Received: October 31 1995 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation L. A. del Rio, J. M. Palma, L. M. Sandalio, F. J. Corpas, G. M. Pastori, P. Bueno, E. López-Huertas; Peroxisomes as a source of superoxide and hydrogen peroxide in stressed plants. Biochem Soc Trans 1 May 1996; 24 (2): 434–438. doi: https://doi.org/10.1042/bst0240434 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: SOD, superoxide dismutase, PMP, peroxisomal membrane polypeptide This content is only available as a PDF. © 1996 Biochemical Society1996 Article PDF first page preview Close Modal You do not currently have access to this content.
Serum Prostate Specific Antigen Levels During Hyperthermia Treatment of Benign Prostatic Hyperplasia (1990)

To understand better the tissue and cell changes occurring in the human prostate as a reaction to heat, we used circulating prostate specific antigen as a marker to detect cell injury. The prostate in 18 patients with benign prostatic hyperplasia was heated to 42 +/- 1.5C with local microwaves at 915 MHz. Each treatment sessions lasted 1 hour and the patients underwent 5 treatment sessions. A total of 90 treatment sessions was performed among 18 patients. No significant difference was observed between pre-treatment and post-treatment serum prostate specific antigen levels. We conclude that local hyperthermia is an atraumatic treatment modality that does not exert its effect on the hyperplastic prostate via a cytotoxic insult to prostate epithelial tissues.
Structural and functional investigations of the murine cavernosal nerve: a model system for serial spatio‐temporal study of autonomic neuropathy (2007)

These results show that it is possible to perform integrated cavernosal pressure monitoring and ultrastructural and electrophysiological studies in this model. These yielded accurate data about the erectile status of the penis, and the state of unmyelinated and myelinated fibres in the DNP and cavernosal nerves of the same animal. This study provides a useful template for future studies of experimental diabetic autonomic neuropathy.
Type II collagen-induced arthritis in mice. I. Major histocompatibility complex (I region) linkage and antibody correlates. (1981)

A model of arthritis was established by the injection of type II collagen into mice. Only mice bearing the H-2q haplotype were susceptible to the disease. Susceptibility was further mapped by the use of recombinant strains on the Iq locus. Type II collagen arthritis was observed in the (resistant X susceptible) F1 cross. Mice strains were designated high, intermediate, or low responders with respect to the anti-type II antibody levels measured by radioimmunoassay. Arthritis-susceptible strains were all classified as high antibody responders. The clinical and histological appearance of type II collagen arthritis in the mouse indicates that it may be a good animal model for the investigation of various immunogenetic traits in rheumatoid arthritis.
Shape and model from specular motion (2002)

This work investigates visual characteristics of specular surfaces during rotation, and gives approaches to qualitatively identify and quantitatively recover shapes of these surfaces. Continuous images are taken when an object rotates. We look at specularly reflected patterns on the surfaces and their motion in the EPIs parallel to the rotation plane, from which estimation of each surface point and construction of the object model are carried out. We find very simple and direct methods to fulfill this objective; linear equations for multiple lights illumination, and a 1st-order differential equation for single light illumination. The motion of specular reflection has nice global characteristics in EPI. The surface types range from very shiny metal surfaces to surfaces with only week specular reflectance. We give both simulation and experiments on real objects.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Bovine cartilage types VI and IX collagens. Characterization of their forms in vivo (1989)

1. Collagens were extracted from bovine cartilage by 4 M-guanidinium chloride in the presence of proteinase inhibitors and identified by immunoblotting with specific anti-collagen sera. 2. The collagens retained their native conformations (shown by the resistance of their triple-helical domains to pepsin digestion), and the molecular masses of their component alpha-chains indicated that the chains were intact. 3. Type VI collagen was extracted as a large-molecular-mass disulphide-bonded aggregate composed of components of molecular mass 140 kDa and 200-240 kDa, and was therefore similar to type VI collagen identified in noncartilaginous tissues. Immunoblotting established the 200-240 kDa components as intact forms of the alpha 3(VI) chain. 4. Type IX collagen consisted of three clearly separable components of molecular mass 84 kDa, 72 kDa and 66 kDa, which were assigned to the alpha 1(IX)-, alpha 3(IX)- and alpha 2(IX)-chains respectively, and a large proportion of this collagen had no covalently bound glycosaminoglycan attached to the alpha 2(IX)-chain. 5. Differences between the type IX collagen extracted from bovine cartilage and that identified in biosynthetic studies on chick cartilage are discussed.
Genetic Variability for Flooding Tolerance in Soybeans (1994)

The flooding tolerance of U.S. soybean [ Glycine max (L.) Merr.] cultivars under field conditions has not been reported. In this study, 84 soybean cultivars of maturity groups II, III, and IV were grown at two locations, London and Lakeview, in Ohio. The cultivars differed in their resistance and tolerance to Phytophthora sojae M.J. Kaufmann &amp; J. W. Gerdemann as determined in separate field and laboratory studies. While the production practices and historic yield potential at the two locations were similar, the nearly flat, slowly permeable Milford silty clay soil at Lakeview was saturated during most of July 1992 because of excessive rainfall (17 cm more than normal). The average yield of the 84 soybean cultivars at the flooded location (Lakeview) was 25% lower than at the non‐flooded location (London). Flooding tolerance can be defined as high yield under flooding stress. When the 84 soybean cultivars were ranked in their flooding tolerance according to this definition, many cultivars were found to exhibit both flood tolerance at Lakeview and high yield at London. Flooding tolerance was independent from Phytophthora tolerance, Phytophthora resistance, and plant relative maturity. This study documented that differences in flooding tolerance exist among U.S. soybean cultivars.
An effective mechanism for supporting content-based routing in scalable Web server clusters (2003)

Clustered server architecture has been employed for many years on the Internet as a way to increase performance, reliability and scalability in the presence of the Internet's explosive growth. Routing mechanism for mapping requests to individual servers within cluster is at the heart of any server clustering techniques. In this paper, we first analyze the deficiencies of existing request routing approaches. Based on these observations, we argue that the request routing mechanism in the cluster-based server should factor in the content of a request in making decisions. Thus, we design and implement a new mechanism to efficiently support content-based routing in the Web server clusters. Performance evaluation on a prototype implementation demonstrates substantial performance improvements over contemporary routing scheme that use weighted round-robin to distribute requests. The proposed mechanism can enable many new capabilities in cluster-based server, such as sophisticated load balancing, differentiated service, special content deployment, session integrity, etc.
Definitions of remission for rheumatoid arthritis and review of selected clinical cohorts and randomised clinical trials for the rate of remission. (2007)

Various definitions of remission in rheumatoid arthritis (RA) have been proposed. The ACR (American College of Rheumatology--formerly ARA, American Rheumatism Association) remission criteria are strict and include nonspecific symptoms such as fatigue. More recently remission according to the Disease Activity Index (DAS) and DAS28 has been described. However, patients who meet the DAS28 remission cut point of < 2.6 may nonetheless have tender and/or swollen joints. The ACR remission criteria are more rigorous than the requirement of DAS28 <2.6. Newer tools for evaluation of RA activity include the Simplified Disease Activity Index (SDAI) and Clinical Disease Activity Index (CDAI), and cut points for remission according to these new indices have been defined. However, all available remission criteria may ignore important aspects of RA, including physical function and radiographic damage.
Retroviral vector design for long-term expression in murine hematopoietic cells in vivo (1994)

A series of retroviral vectors containing the human glucocerebrosidase (GC) cDNA driven by various promoters have been constructed in an attempt to discover which vector design can most efficiently transduce murine hematopoietic stem cells (HSCs) and drive expression of the transferred gene in hematopoietic cells of mice reconstituted with the transduced stem cells. The simplest vector, LG, in which the GC gene is driven by the viral LTR, was the most efficient vector at infecting HSCs, with an average viral copy number in hematopoietic tissues of 3 copies/cell in recipient mice. In general, the viral vectors that contained any additional promoters or enhancers to drive expression of either the GC gene or a selectable marker gene (Neo) had lower titers and/or transduced HSCs at a lower efficiency. This was seen most markedly when the human phosphoglycerate (PGK) promoter was used to drive the human GC cDNA. Despite repeated attempts to obtain a high titer producer clone, this virus consistently produced low titers and subsequently resulted in the lowest proviral copy numbers in long-term reconstituted mice. Only the viral LTR and PGK promoter were capable of driving significant levels of human GC RNA in hematopoietic cells of long-term reconstituted mice, with a much lower level of RNA generated by an internal herpes TK or SV40 immediate early promoter. Insertion of the internal transcription unit in the opposite orientation relative to the viral LTRs had a detrimental effect on gene expression. The levels of RNA generated by a hybrid LTR containing the myeloproliferative sarcoma virus enhancer were higher in bone marrow-derived macrophages than in nonadherent cells of the bone marrow when compared with the LG vector. The presence of an internal promoter to drive expression of the human GC cDNA did not seem to have a detrimental effect on expression levels from the viral LTR. In fact, in the presence of an internal TK or PGK promoter expression from the LTR was increased despite the presence of lower proviral copy numbers. Insertion of a second gene (Neo) into the vector had a negative impact on long-term expression in hematopoietic cells in vivo; however, this seems to be due solely to the lower transduction efficiency of this vector. Overall, the highest levels of GC activity in macrophages of long-term reconstituted mice were generated by the LG vector; however, these levels were variable.(ABSTRACT TRUNCATED AT 400 WORDS)
Mapping of Candida albicans oligomannosidic epitopes by using monoclonal antibodies (1992)

Six monoclonal antibodies (MAbs) from various laboratory sources (EB-CA1, EB-CA2, H5, AF1, C6, and 5B2), reacting with the polysaccharidic moieties of Candida albicans mannoproteins, were used for epitope mapping by an enzyme-linked immunosorbent assay (ELISA) with neoglycolipids and by Western blotting (immunoblotting) of a C. albicans germ tube extract. The ELISA involved neoglycolipids constructed from three families of oligomannosides released by sequential depolymerization of C. albicans phosphopeptidomannan by acid hydrolysis (NGLH), beta-elimination (NGLO), and acetolysis (NGLA). All of the MAbs exhibited low reactivities against NGLO. MAbs EB-CA1, EB-CA2, and H5 reacted mainly against NGLA, and MAbs C6 and AF1 recognized mainly NGLH, whereas MAb 5B2 reacted with both families of neoantigens. When this method was compared with Western blotting, strong reactivity to NGLA was associated with the presence of epitopes shared by high-molecular-weight mannoproteins, whereas strong reactivity to NGLH was associated with a reactivity to a family of 14- to 18-kDa antigens. The reactivity of MAb 5B2 was associated with both high-molecular-weight mannoproteins and the 14- to 18-kDa antigens. In relation to the present knowledge about the structure of the C. albicans phosphopeptidomannan oligomannosidic repertoire, these results provide preliminary data concerning the molecular basis of the recognition of mannopyranosyl sequences by MAbs and their distribution among C. albicans mannoproteins.
Operation of Graphene Transistors at Gigahertz Frequencies (2008)

Top-gated graphene transistors operating at high frequencies (gigahertz) have been fabricated and their characteristics analyzed. The measured intrinsic current gain shows an ideal 1/f frequency dependence, indicating a FET-like behavior for graphene transistors. The cutoff frequency f(T) is found to be proportional to the dc transconductance g(m) of the device, consistent with the relation f(T) = g(m)/(2piC(G)). The peak f(T) increases with a reduced gate length, and f(T) as high as 26 GHz is measured for a graphene transistor with a gate length of 150 nm. The work represents a significant step toward the realization of graphene-based electronics for high-frequency applications.
Introductory lecture. Studies of the liquid/solid interface by scanning tunnelling microscopy and scanning electrochemical microscopy (1992)

The application of scanning tunnelling microscopy (STM) and scanning electrochemical microscopy (SECM) to studies of the liquid/solid interface, especially electrode surfaces, is reviewed. Some general principles describing features of the images obtained by these techniques are proposed and illustrated by examples taken from recent work in the authors' laboratory.
Mitochondrial abnormalities in Alzheimer brain: Mechanistic implications (2005)

Reductions in cerebral metabolism sufficient to impair cognition in normal individuals also occur in Alzheimer's disease (AD). The degree of clinical disability in AD correlates closely to the magnitude of the reduction in brain metabolism. Therefore, we tested whether impairments in tricarboxylic acid (TCA) cycle enzymes of mitochondria correlate with disability. Brains were from patients with autopsy-confirmed AD and clinical dementia ratings (CDRs) before death. Significant (p < 0.01) decreases occurred in the activities of the pyruvate dehydrogenase complex (-41%), isocitrate dehydrogenase (-27%), and the alpha-ketoglutarate dehydrogenase complex (-57%). Activities of succinate dehydrogenase (complex II) (+44%) and malate dehydrogenase (+54%) were increased (p < 0.01). Activities of the other four TCA cycle enzymes were unchanged. All of the changes in TCA cycle activities correlated with the clinical state (p < 0.01), suggesting a coordinated mitochondrial alteration. The highest correlation was with pyruvate dehydrogenase complex (r = 0.77, r2= 0.59). Measures to improve TCA cycle metabolism might benefit AD patients.
Net by Net Routing with a New Path Search Algorithm (2000)

Net by net routing is still a very important technique used to make connections in VLSI circuits. The maze routing algorithms used for this purpose correspond to shortest path searches derived from basic BFS or from A*, with many dedicated improvements. This paper proposes the use of a new path search algorithm, LCS*, for routing individual connections in VLSI circuits. LCS* is a generic and simultaneous bidirectional heuristic algorithm which is faster than A* in most graph domains, such as VLSI routing grids. This is achieved by using dynamic estimation with the separation of computed values for open and closed nodes. Results show that the running time is reduced and little storage space is needed.
Gene V Protein Dimerization and Cooperativity of Binding to Poly(dA) (1996)

Gene V protein of bacteriophage f1 is a dimeric protein that binds cooperatively to single-stranded nucleic acids. In order to determine whether a monomer-dimer equilibrium has an appreciable effect upon the thermodynamics of gene V protein binding to nucleic acids, the dissociation constant for the protein dimer was investigated using size-exclusion chromatography. At concentrations ranging from 5 x 10(-10) to 1.2 x 10(-5) M, the Stokes radius of the protein was that expected of the dimer of the gene V protein. The Stokes radius of the protein was also independent of salt concentration from 0.2 to 1.0 M NaCl in a buffer containing 10 mM Tris-HCl, pH 7.4, and 1 mM EDTA. The binding of the dimeric gene V protein to poly(dA) was studied using a simplified lattice model for protein-protein interactions adapted for use with a dimeric protein that binds simultaneously to two strands of nucleic acid. Interpretation of the salt dependence, C = [d log(Kint omega)]/[d log(NaCl)], of binding of such a dimeric protein to nucleic acid using the theory of Record et al. (Record, M. T., et al. (1976) J. Mol. Biol. 107, 145-158) indicates that C is a function of the numbers of cations and anions released from protein and nucleic acid upon binding of the dimer, not of the monomer. Cooperativity of gene V protein binding to poly(dA) was studied with titration experiments that are sensitive to the degree of cooperativity of binding. The cooperativity factor omega, defined as the ratio of the binding constant for a site adjacent to a previously bound dimer to that for an isolated site, was found to be relatively insensitive to salt, with a value in the range of 2000-7000 for binding to poly(dA) at 3 degrees C and at 23 degrees C. This high cooperativity factor supports the suggestion that protein-protein contacts play a major role in the formation of the superhelical gene V protein-single-stranded nucleic acid complex.
Acceleration Effect of Lewis Acid in Allylboration of Aldehydes:  Catalytic, Regiospecific, Diastereospecific, and Enantioselective Synthesis of Homoallyl Alcohols (2002)

The addition of pinacol allylboronic esters to aromatic and aliphatic aldehydes smoothly occurred at -78 degrees C in toluene in the presence of a catalytic amount of AlCl3 or Sc(OTf)3 (10 mol %) to give the corresponding homoallyl alcohols in high yields. The reactions proceeded regio- and diastereospecifically, yielding the isomerically pure syn- and anti-homoallyl alcohols from (Z)- and (E)-allylboronic esters, respectively. The protocol was also applied to enantioselective reactions by using a chiral Lewis acid catalyst.
The gene for autosomal dominant spinocerebellar ataxia (SCA1) maps telomeric to the HLA complex and is closely linked to the D6S89 locus in three large kindreds. (1991)

We studied three large kindreds with the HLA-linked form of spinocerebellar ataxia (SCA1) in order to localize the SCA1 locus on the short arm of chromosome 6 (6p). Two loci containing highly informative dinucleotide repeat sequences were used for linkage analysis. These two loci are D6S89, which is telomeric to the HLA region, and T complex-associated testes-expressed 1 (TCTE1), centromeric to HLA. Pairwise linkage analysis of SCA1 and D6S89 revealed a maximum lod score of 5.86 in the Houston SCA1 (HSCA1) kindred and of 8.08 in the Calabrian SCA1 (SCA1) kindreds, at recombination fractions of .050 and .022, respectively. A maximum pairwise lod score of 4.54 at a recombination frequency of .100 was obtained for SCA1 and TCTE1 in the HSCA1 kindred. No evidence for linkage was detected between TCTE1 and SCA1 in the CSCA1 kindreds. Multilocus linkage analysis of SCA1, HLA, and D6S89 in all three kindreds provided strong evidence for localization of the SCA1 locus telomeric to the HLA regions. However, multilocus linkage analysis of SCA1, HLA, and TCTE1 with HSCA1 family genotypes indicated the possibility of a location of the SCA1 locus centromeric to HLA. An analysis of HSCA1 recombinants in this region of chromosome 6 revealed relatively high recombination frequencies between HLA and each of the other two markers and relatively low frequencies between the latter and SCA1, predicting that the SCA1 locus would tend to segregate away from HLA together with D6S89 or TCTE1, as found with the three-point linkage analyses for this family.(ABSTRACT TRUNCATED AT 250 WORDS)