gebhart/patrepeval huggingface dataset:
Optimizing heart failure therapy with implantable sensors (2012)

Abstract Heart failure (HF)‐related hospitalization is associated with significant mortality and morbidity and can be prevented by early intervention. Implantable sensors detect early pathophysiological changes in HF, using an accelerometer, a paced electrogram, impedance and pressure sensors in implanted intracardiac leads, or stand‐alone devices. Such sensors monitor daily activity, QT and ST intervals, pulmonary fluid, and intracardiac pressures at various points. Sensor data are available either by patient's or physician's regular interrogation, or using remote patient monitoring. Different sensors have different levels of sensitivity and specificity for HF detection, and they have the ability to antedate HF exacerbation and thereby allow for the initiation of intervention to avert decompensation. Clinical studies suggest that alone or in combination, such sensors have a greater beneficial impact than conventional therapy on acute HF outcome.
Dependence of Phosphorescent Emitter Orientation on Deposition Technique in Doped Organic Films (2016)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTDependence of Phosphorescent Emitter Orientation on Deposition Technique in Doped Organic FilmsThomas Lampe*†, Tobias D. Schmidt†, Matthew J. Jurow‡, Peter I. Djurovich‡, Mark E. Thompson‡, and Wolfgang Brütting*†View Author Information† Institute of Physics, University of Augsburg, 86135 Augsburg, Germany‡ Department of Chemistry, University of Southern California, Los Angeles, California 90089, United States*T. Lampe. E-mail: [email protected]*W. Brütting. E-mail: [email protected]Cite this: Chem. Mater. 2016, 28, 3, 712–715Publication Date (Web):January 21, 2016Publication History Received27 November 2015Revised21 January 2016Published online26 January 2016Published inissue 9 February 2016https://pubs.acs.org/doi/10.1021/acs.chemmater.5b04607https://doi.org/10.1021/acs.chemmater.5b04607rapid-communicationACS PublicationsCopyright © 2016 American Chemical SocietyRequest reuse permissionsArticle Views2319Altmetric-Citations54LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Deposition,Layers,Materials,Molecules,Quantum mechanics Get e-Alerts
SNP discovery via 454 transcriptome sequencing (2007)

A massively parallel pyro-sequencing technology commercialized by 454 Life Sciences Corporation was used to sequence the transcriptomes of shoot apical meristems isolated from two inbred lines of maize using laser capture microdissection (LCM). A computational pipeline that uses the POLYBAYES polymorphism detection system was adapted for 454 ESTs and used to detect SNPs (single nucleotide polymorphisms) between the two inbred lines. Putative SNPs were computationally identified using 260,000 and 280,000 454 ESTs from the B73 and Mo17 inbred lines, respectively. Over 36,000 putative SNPs were detected within 9980 unique B73 genomic anchor sequences (MAGIs). Stringent post-processing reduced this number to > 7000 putative SNPs. Over 85% (94/110) of a sample of these putative SNPs were successfully validated by Sanger sequencing. Based on this validation rate, this pilot experiment conservatively identified > 4900 valid SNPs within > 2400 maize genes. These results demonstrate that 454-based transcriptome sequencing is an excellent method for the high-throughput acquisition of gene-associated SNPs.
Resolution in Plenoptic Cameras (2009)

Derivation and analysis of sampling patterns of traditional and focused plenoptic cameras show the former rotates pixels π/2 in phase space, while the latter does not. These results are interpreted regarding the cameras’ spatial resolution.
Mesenchymal Stem Cells in Osteobiology and Applied Bone Regeneration (1998)

Bone marrow contains a population of rare progenitor cells capable of differentiating into bone, cartilage, muscle, tendon, and other connective tissues. These cells, referred to as MSCs, can be purified and culture expanded from animals and humans. This review summarizes recent experimentation focused on characterizing the cellular aspects of osteogenic differentiation, and exploration of the potential for using autologous stem cell therapy to augment bone repair and regeneration. The authors have completed an array of preclinical studies showing the feasibility and efficacy of MSC based implants to heal large osseous defects. After confirming that syngeneic rat MSCs could heal a critical size segmental defect in the femur, it was established that human MSCs form bone of considerable mechanical integrity when implanted in an osseous defect in an immunocompromised animal. Furthermore, bone repair studies in dogs verify that the technology is transferable to large animals, and that the application of this technology to patients at geographically remote sites is feasible. These studies suggest that by combining MSCs with an appropriate delivery vehicle, it may be possible to offer patients new therapeutic options.
Characterization of transposable element-associated mutations that alter yeast alcohol dehydrogenase II expression. (1983)

Seven cis-dominant, constitutively expressed mutations of the normally glucose-repressible isozyme of alcohol dehydrogenase (ADHII) from the yeast Saccharomyces cerevisiae are caused by insertion of transposable elements from the Ty1 family in front of the ADHII structural gene (ADR2) (V. M. Williamson, E. T. Young, and M. Ciriacy, Cell 23:605-614, 1981). We cloned ADR2 with its associated Ty1 element from five S. cerevisiae strains carrying these mutations. Comparison of the Ty1 elements by heteroduplex studies and restriction enzyme analyses indicated that four were very similar; the fifth, although the same size as the others (about 5.6 kilobases), differed by the presence of two large substitutions of approximately 1 and 2 kilobases. The DNA sequences of the terminal direct repeats (deltas) were very homologous but not identical and were similar to previously reported Ty1 element direct repeats. We determined the 5'-flanking sequences of the ADR2 gene isolated from a wild-type strain and from five Ty1-associated mutations. The 5-base pair target sequence at the site of Ty1 insertion was present at both ends of each Ty1 element. The sites of insertion of the elements were all different and occurred from 125 to 210 base pairs in front of the coding region of ADR2. The 5' end of the major transcript as determined by S1 mapping was the same in wild-type cells and in Ty1-associated constitutive mutants and was approximately 54 base pairs upstream from the coding region. ADR2 transcripts were not detected when a solo delta sequence was present in the 5'-flanking region of this gene.
Identification of residues in the V domain of CD80 (B7-1) implicated in functional interactions with CD28 and CTLA4. (1995)

The CD80 (B7-1) molecule is a 45-60-kD member of the immunoglobulin superfamily that is expressed on a variety of cell types of haematopoietic origin. CD80 can provide a critical costimulatory signal to T cells by interacting with the T cell surface molecule CD28. CD80 also binds to the CD28-related molecule CTLA4, which is expressed on activated T cells, Recently, additional ligands of CD28 and CTLA4 have been described in mice and humans. One of them, CD86 (B-70 or B7-2) was characterized at the molecular level. Although similar in predicted structure to CD80, it is distantly related in amino acid sequence. In this study, human CD80 mutants were generated and tested for their ability to maintain the interaction with CD28 leading to adhesion and enhanced IL-2 production. Two hydrophobic residues in the V-like domain of CD80 were identified as critical for binding to CD28 and are also important for the interaction with CTLA4. These residues are adjacent to the epitope of the BB1 antibody, which inhibits CD28-CD80 interactions. One of these residues, Y87, is conserved in all CD80 and CD86 cloned from various species. These results being to unravel the structural requirements for binding to CD28 and CTLA4.
Tyrosine phosphorylation in Myxococcus xanthus, a multicellular prokaryote (1996)

Tyrosine phosphorylation is an extremely rare event in prokaryotes, occurring almost exclusively in multicellular eukaryotes. We have identified, for the first time, by the use of antiphosphotyrosine monoclonal antibody and Western blot (immunoblot) analysis, two tyrosine-phosphorylated membrane proteins in the multicellular prokaryote Myxococcus xanthus. The pattern of tyrosine phosphorylation was shown to change during development, indicating a possible role for this regulatory modification during two stages of development, i.e., aggregation and sporulation. Furthermore, the altered pattern of tyrosine phosphorylation observed in a variety of signaling mutants was shown to differ from that observed in the wild type, suggesting further the possible involvement of tyrosine phosphorylation during the development program.
Quantum cryptographic system based on silica-based planar lightwave circuits (2004)

We constructed a fast-data-encoding quantum cryptographic system using integrated-optic interferometers based on planar lightwave circuit technology, and confirmed its operation and stability. Experimental results show that our system is useful for implementing a practical four-state quantum key distribution system.
Self-assembled polyelectrolyte multilayer modified Nafion membrane with suppressed vanadium ion crossover for vanadium redox flow batteries (2008)

The crossover of vanadium ions through proton-exchange membranes such as those of Nafion is the chief reason that results in the low energy efficiency and high self-discharge rate of vanadium redox flow batteries (VRB). With respect to applicability, the ideal proton-exchange membrane used in VRB should possess simultaneously high proton conductivity and low vanadium ion permeability. Here, we report a novel approach using a polyelectrolyte layer-by-layer self-assembly technique to fabricate a barrier layer onto the surface of Nafion membrane by alternate adsorption of polycation poly(diallyldimethylammonium chloride) (PDDA) and polyanion poly(sodium styrene sulfonate) (PSS), which can suppress the crossover of vanadium ions. The Nafion–[PDDA-PSS]n membrane (n = the number of multilayers) obtained shows much lower vanadium ion permeability compared with plain Nafion membrane. Accordingly, the VRB with Nafion–[PDDA-PSS]n membrane exhibits a higher coulombic efficiency (CE) and energy efficiency (EE) together with a slower self-discharge rate than that of Nafion system. The highest CE of 97.6% and EE of 83.9% can be achieved at charge–discharge current density of 80 mA cm−2 and 20 mA cm−2, respectively.
Long-term results of interstitial implant in locally advanced cervical cancer:A retrospective study of 50 cases (1996)

A retrospective study was conducted to assess the impact of different clinical parameters on the survival (S), disease free survival (DFS), pelvic recurrence (LR), distant metastases (DM), and the development of major complications in 50 patients with locally advanced cervical cancer. The parameters were: early stage (I–II) vs. late (III–IV); tumor size, ≤6 cm vs. >6 cm; one interstitial implant (T) vs. two T vs. T + tandem-ovoid insertion (T/O); dose to point A, ≤8000 cGy vs. >8000 cGy; dose to point B, ≤6500 cGy vs. >6500 cGy; dose to point P, ≤5500 cGy vs. >5500 cGy; bladder and rectum dose, ≤7000 cGy vs. >7000 cGy; chemotherapy vs. no chemotherapy. The median age was 48 years and the median follow-up was 23.5 months. The treatment consisted of external irradiation, low dose rate T alone (34 patients) or in combination with T/O (16 patients). Radiation and chemotherapy (CT) were given in 28 patients while 22 had no CT. At 60 months, the patients with an early and late stage had S rates of 57% and 17% respectively, P = 0.0071 and DFS rates of 57% and 22% respectively, P = 0.0031. While LR was not significantly different between patients with early and late stage, P = 0.1807, DM was, P = 0.0067. No other variable was statistically correlated with S, DFS and LR. For the whole group, LR was 42%. Twenty-two patients (44%) had DM. Six patients (12% crude incidence rate) experienced major complications: two rectovaginal fistulae, one rectal ulceration and two small bowel obstructions requiring colostomy and one hemorrhagic cystitis requiring hospitalization. Radiat Oncol Invest 1996;4:33–40. © 1996 Wiley-Liss, Inc.
A review of bispecific antibodies and antibody constructs in oncology and clinical challenges (2019)

Bispecific antibodies (bsAbs) are antibodies that bind two distinct epitopes to cancer.. For use in oncology, one bsAb has been approved and 57 bsAbs are in clinical trials, none of which has reached phase 3. These bsAbs show great variability in design and mechanism of action. The various designs are often linked to the mechanisms of actions. The majority of bsAbs engage immune cells to destroy tumor cells. However, some bsAbs are also used to deliver payloads to tumors or to block tumor signaling pathways. This review provides insight into the choice of construct for bsAbs, summarizes the clinical development of bsAbs in oncology and identifies subsequent challenges.
Construction of a P450c27 fusion enzyme: a useful tool for analysis of vitamin D3 25-hydroxylase activity (1996)

Liver mitochondrial P450c27, encoded by the CYP27 gene, can catalyse the 25-hydroxylation of vitamin D3 and the 27-hydroxylation of sterols. To facilitate the study of this enzyme in cell culture systems, we engineered a fusion protein consisting of P450c27 coupled to its electron-transport accessory proteins, ferredoxin and ferredoxin reductase, and assessed its enzyme activity by measuring the C-25 and C-27 (side-chain) hydroxylation of 1 alpha-hydroxyvitamin D3 (1 alpha-OH-D3). When incubated with 1 alpha-OH-D3, COS-1 cells transfected with a vector expressing the fusion protein produced 1 alpha,25-(OH)2D2 and 1 alpha,27-(OH)2D3 about four times more efficiently than did cells transfected with three individual components of the fusion. When incubated with the natural substrate, vitamin D3, the efficiency of hydroxylation was lower than that for 1 alpha-OH-D3 but still 1.7-fold higher for the fusion protein than for its individual components. The fusion protein was also able to reproduce qualitatively and quantitatively the activity shown by P450c27 in liver cells in situ. The P450c27-ferredoxin reductase-ferredoxin fusion construct represents a valuable tool for establishing the substrate specificity of this liver cytochrome and for evaluating its potential for activating pro-drug analogues of vitamin D.
αVβ3 Integrin-Targeted Radionuclide Therapy with 64Cu-cyclam-RAFT-c(-RGDfK-)4 (2016)

Abstract The transmembrane cell adhesion receptor αVβ3 integrin (αVβ3) has been identified as an important molecular target for cancer imaging and therapy. We have developed a tetrameric cyclic RGD (Arg-Gly-Asp) peptide–based radiotracer 64Cu-cyclam-RAFT-c(-RGDfK-)4, which successfully captured αVβ3-positive tumors and angiogenesis by PET. Here, we subsequently evaluated its therapeutic potential and side effects using an established αVβ3-positive tumor mouse model. Mice with subcutaneous U87MG glioblastoma xenografts received single administrations of 37 and 74 MBq of 64Cu-cyclam-RAFT-c(-RGDfK-)4 (37 MBq/nmol), peptide control, or vehicle solution and underwent tumor growth evaluation. Side effects were assessed in tumor-bearing and tumor-free mice in terms of body weight, routine hematology, and hepatorenal functions. Biodistribution of 64Cu-cyclam-RAFT-c(-RGDfK-)4 with ascending peptide doses (0.25–10 nmol) and with the therapeutic dose of 2 nmol were determined at 3 hours and at various time points (2 minutes–24 hours) postinjection, respectively, based on which radiation-absorbed doses were estimated. The results revealed that 64Cu-cyclam-RAFT-c(-RGDfK-)4 dose dependently slowed down the tumor growth. The mean tumor doses were 1.28 and 1.81 Gy from 37 and 74 MBq of 64Cu-cyclam-RAFT-c(-RGDfK-)4, respectively. Peptide dose study showed that the tumor uptake of 64Cu-cyclam-RAFT-c(-RGDfK-)4 dose dependently decreased at doses ≥1 nmol, indicating a saturation of αVβ3 with the administered therapeutic doses (1 and 2 nmol). Combined analysis of the data from tumor-bearing and tumor-free mice revealed no significant toxicity caused by 37–74 MBq of 64Cu-cyclam-RAFT-c(-RGDfK-)4. Our study demonstrates the therapeutic efficacy and safety of 64Cu-cyclam-RAFT-c(-RGDfK-)4 for αVβ3-targeted radionuclide therapy. 64Cu-cyclam-RAFT-c(-RGDfK-)4 would be a promising theranostic drug for cancer imaging and therapy. Mol Cancer Ther; 15(9); 2076–85. ©2016 AACR.
Prostaglandins Promote and Block Adipogenesis through Opposing Effects on Peroxisome Proliferator-activated Receptor γ (1998)

Fat cell differentiation is a critical aspect of obesity and diabetes. Dietary fatty acids are converted to arachidonic acid, which serves as precursor of prostaglandins (PGs). PGJ2 derivatives function as activating ligands for peroxisome proliferator-activated receptor gamma (PPAR gamma), a nuclear hormone receptor that is central to adipogenic determination. We report here that PGF2 alpha blocks adipogenesis through activation of mitogen-activated protein kinase, resulting in inhibitory phosphorylation of PPAR gamma. Both mitogen-activated protein kinase activation and PPAR gamma phosphorylation are required for the anti-adipogenic effects of PGF2 alpha. Thus, PG signals generated at a cell surface receptor regulate the program of gene expression required for adipogenesis by modulating the activity of a nuclear hormone receptor that is directly activated by other PG signals. The balance between PGF2 alpha and PGJ2 signaling may thus be central to the development of obesity and diabetes.
Rapid Molecular Characterization of Clostridium difficile and Assessment of Populations of C. difficile in Stool Specimens (2009)

Our laboratory has developed testing methods that use real-time PCR and pyrosequencing analysis to enable the rapid identification of potential hypervirulent Clostridium difficile strains. We describe a real-time PCR assay that detects four C. difficile genes encoding toxins A (tcdA) and B (tcdB) and the binary toxin genes (cdtA and cdtB), as well as a pyrosequencing assay that detects common deletions in the tcdC gene in less than 4 h. A subset of historical and recent C. difficile isolates (n = 31) was also analyzed by pulsed-field gel electrophoresis to determine the circulating North American pulsed-field (NAP) types that have been isolated in New York State. Thirteen different NAP types were found among the 31 isolates tested, 13 of which were NAP type 1 strains. To further assess the best approach to utilizing our conventional and molecular methods, we studied the populations of C. difficile in patient stool specimens (n = 23). Our results indicated that 13% of individual stool specimens had heterogeneous populations of C. difficile when we compared the molecular characterization results for multiple bacterial isolates (n = 10). Direct molecular analysis of stool specimens gave results that correlated well with the results obtained with cultured stool specimens; the direct molecular analysis was rapid, informative, and less costly than the testing of multiple patient stool isolates.
Comprehensive support for management of enterprise applications (2008)

During the last decades, performance of available hardware resources constantly increased (Moore, 1965), which enabled the assignment of more and more complex tasks to software systems. As one consequence, the inherent complexity of these software systems also increases, influencing all phases of their lifecycle. The concept of component orientation (CO) (Szyperski, 1999) allows the development of software systems in a modular way through functional decomposition. Administration and maintenance of software systems are addressed by the vision of autonomic computing (AC) (Horn, 2001), based on the idea to assign low level administrative tasks to the system itself. With mKernel an AC-infrastructure for component oriented enterprise applications is provided, based on the enterprise Java bean (EJB) standard, version 3.0 (DeMichiel and Keith, 2006). In contrast to existing approaches, the main advantage of mKernel lies within its standard compliance, not prescribing any additional guidelines for the development of applications to enable their autonomous management. It is realized as plugin for an existing container, not requiring any adjustment of the underlying implementation. Moreover, it provides a very fine grained interface for inspection and manipulation of the managed system, taking the specifics of the supported standard into account. Within this paper we present the opportunities provided by mKernel to control a managed system.
Temperature corrected thromboelastometry in hypothermic trauma patients (2008)

Delhaye, O.; Wavreille, G.; Tournoys, A.; Garrigue, D.; Tavernier, B. Author Information
Interdependency of Brassinosteroid and Auxin Signaling in Arabidopsis (2004)

How growth regulators provoke context-specific signals is a fundamental question in developmental biology. In plants, both auxin and brassinosteroids (BRs) promote cell expansion, and it was thought that they activated this process through independent mechanisms. In this work, we describe a shared auxin:BR pathway required for seedling growth. Genetic, physiological, and genomic analyses demonstrate that response from one pathway requires the function of the other, and that this interdependence does not act at the level of hormone biosynthetic control. Increased auxin levels saturate the BR-stimulated growth response and greatly reduce BR effects on gene expression. Integration of these two pathways is downstream from BES1 and Aux/IAA proteins, the last known regulatory factors acting downstream of each hormone, and is likely to occur directly on the promoters of auxin:BR target genes. We have developed a new approach to identify potential regulatory elements acting in each hormone pathway, as well as in the shared auxin:BR pathway. We show that one element highly overrepresented in the promoters of auxin- and BR-induced genes is responsive to both hormones and requires BR biosynthesis for normal expression. This work fundamentally alters our view of BR and auxin signaling and describes a powerful new approach to identify regulatory elements required for response to specific stimuli.
Specific Inhibition of Viral Ribonucleic Acid Replication by Gliotoxin (1968)

Gliotoxin inhibits intracellular replication of poliovirus in HeLa cells at a stage subsequent to adsorption and penetration of virus. The sensitive step is synthesis of viral RNA: synthesis of viral protein is unaffected except as a consequence of blockade of RNA synthesis. Concentrations of gliotoxin sufficient to block viral RNA synthesis completely do not affect cellular RNA synthesis.