gebhart/patrepeval huggingface dataset:
Imaging brain amyloid in Alzheimer's disease with Pittsburgh Compound‐B (2004)

This report describes the first human study of a novel amyloid-imaging positron emission tomography (PET) tracer, termed Pittsburgh Compound-B (PIB), in 16 patients with diagnosed mild AD and 9 controls. Compared with controls, AD patients typically showed marked retention of PIB in areas of association cortex known to contain large amounts of amyloid deposits in AD. In the AD patient group, PIB retention was increased most prominently in frontal cortex (1.94-fold, p = 0.0001). Large increases also were observed in parietal (1.71-fold, p = 0.0002), temporal (1.52-fold, p = 0.002), and occipital (1.54-fold, p = 0.002) cortex and the striatum (1.76-fold, p = 0.0001). PIB retention was equivalent in AD patients and controls in areas known to be relatively unaffected by amyloid deposition (such as subcortical white matter, pons, and cerebellum). Studies in three young (21 years) and six older healthy controls (69.5 +/- 11 years) showed low PIB retention in cortical areas and no significant group differences between young and older controls. In cortical areas, PIB retention correlated inversely with cerebral glucose metabolism determined with 18F-fluorodeoxyglucose. This relationship was most robust in the parietal cortex (r = -0.72; p = 0.0001). The results suggest that PET imaging with the novel tracer, PIB, can provide quantitative information on amyloid deposits in living subjects.
Alternate splicing of mRNAs encoding human mast cell growth factor and localization of the gene to chromosome 12q22-q24. (1991)

Human mast cell growth factor (MGF) complementary DNAs (cDNAs) were cloned from HeLa cells using the polymerase chain reaction with oligonucleotides corresponding to murine and human MGF sequences. Sequencing of the cloned human MGF polymerase chain reaction products revealed two types of cDNA: a full length form corresponding in size to the murine cDNA, and an alternately spliced clone with a deletion of the sixth exon of the gene. Since membrane-bound MGF is predicted to be proteolytically cleaved within the sequences encoded by exon 6 to generate a soluble protein, this alternately spliced cDNA would likely encode a noncleavable, membrane-bound form of MGF. No difference in biological activity on human bone marrow cells was observed with recombinant, soluble forms of both types of human MGF protein. Our previous localization of the murine MGF gene to the Sl locus on chromosome 10 suggested (via conserved linkage groups) that the human MGF gene would be located on human chromosome 12. Therefore, rodent-human somatic cell hybrids with or without an entire human chromosome 12 and hybrids retaining partial 12 were tested by Southern blot analysis and used to show the presence of the human Mgf locus at chromosome region 12q. Chromosomal in situ hybridization localized the gene to 12q22-q24 in the region predicted by the comparative mapping of the murine Mgf/Sl locus.
Stent wire cutting during coronary directional atherectomy (1993)

Directional atherectomy represents one potential approach for the treatment of restenosis in stented coronary arteries. In this case report we demonstrate an important pitfall of this angioplasty technique for stent restenosis--inadvertent entanglement of the stent wire in the device. While cutting and removal of part of the wire was achieved in the case presented, this potential adverse event limits the applicability of directional atherectomy for restenosis in coronary stents.
A role for pericytes as microenvironmental regulators of human skin tissue regeneration (2009)

The cellular and molecular microenvironment of epithelial stem and progenitor cells is poorly characterized despite well-documented roles in homeostatic tissue renewal, wound healing, and cancer progression. Here, we demonstrate that, in organotypic cocultures, dermal pericytes substantially enhanced the intrinsically low tissue-regenerative capacity of human epidermal cells that have committed to differentiate and that this enhancement was independent of angiogenesis. We used microarray analysis to identify genes expressed by human dermal pericytes that could potentially promote epidermal regeneration. Using this approach, we identified as a candidate the gene LAMA5, which encodes laminin alpha5, a subunit of the ECM component laminin-511/521 (LM-511/521). LAMA5 was of particular interest as we had previously shown that it promotes skin regeneration both in vitro and in vivo. Analysis using immunogold localization revealed that pericytes synthesized and secreted LAMA5 in human skin. Consistent with this observation, coculture with pericytes enhanced LM-511/521 deposition in the dermal-epidermal junction of organotypic cultures. We further showed that skin pericytes could also act as mesenchymal stem cells, exhibiting the capacity to differentiate into bone, fat, and cartilage lineages in vitro. This study suggests that pericytes represent a potent stem cell population in the skin that is capable of modifying the ECM microenvironment and promoting epidermal tissue renewal from non-stem cells, a previously unsuspected role for pericytes.
Multivalent Anti-CD20/Anti-CD22 Bispecific Antibody Fusion Proteins Made by the DNL Method Show Potent Lymphoma Cytotoxicity. (2006)

Abstract Purpose: Multivalent and either monospecific or bispecific antibodies have shown the potential for improved efficacy in a number of preclinical studies. A panel of such agents based on hLL2 (epratuzumab, anti-CD22) and hA20 (humanized anti-CD20) was made and evaluated for cytotoxicity in vitro and anti-tumor activity in vivo. Methods: The Dock and Lock (DNL) method (Rossi et al., Proc. Natl. Acad. Sci. USA, 2006,103:6841) is a novel platform technology for the site-specific and covalent assembly of modular components. DNL was utilized to generate two hexavalent monospecific (hex-hA20 and hex-hLL2), and two hexavalent bispecific (DNL-1 and DNL-2) stably tethered complexes, each composed of four Fab fragments conjugated to an IgG at the latter’s carboxyl termini of the heavy chain. DNL-1 consists of four hA20 Fab fragments tethered to an hLL2 IgG. DNL-2 consists of four hLL2 Fab fragments tethered to an hA20 IgG. Results: For each construct, the DNL method produced a single, defined, homogeneous structure that is stable in serum. All of the constituent Fab fragments are functional, with binding affinities similar to the parental MAbs. In vitro analyses using human Burkitt lymphoma cell lines demonstrate that both the monospecific and bispecific constructs induce strong cell adhesion and potently inhibit cell growth without the requirements for further cross-linking. DNL-1, DNL-2 and hex-hA20 each completely inhibited the growth of cultured Daudi cells at concentrations as low as 1 nM and exhibited two to three logs greater potency than that of hA20 IgG or rituximab under the same experimental conditions. Anti-tumor efficacy was demonstrated in SCID mice bearing disseminated Daudi lymphoma. Treatment with 4 μg of hex-hA20 or DNL-2 at 1, 4 and 7 days post intravenous injection of 1 × 107 Daudi cells resulted in significantly increased survival of tumor-bearing mice compared to controls treated with saline. Conclusion: These findings suggest that the DNL method can be used to make multivalent proteins with potent anti-tumor activity, as shown here with anti-CD20/anti-CD22 bispecific antibody fusion proteins.
A Multiuser TRDMA Uplink System with 2D Parallel Interference Cancellation (2014)

The concept of Time-Reversal Division Multiple Access (TRDMA) has recently been proposed as a promising medium access technology for wireless broadband communications. Previous work on TRDMA mainly focuses on the multi-user downlink system. In this paper, we first introduce a TRDMA-based multi-user uplink architecture and then propose a 2D parallel interference cancellation scheme to enhance system performance. The TRDMA uplink architecture keeps the cost of end-users at a minimum level, and reuses the processing power at the base station that has already been made available for the downlink. The proposed 2D parallel interference cancellation scheme utilizes tentative decisions of detected symbols to effectively cancel both inter-symbol interference and inter-user interference, which significantly improve the bit-error-rate (BER) performance in high SNR regime. To further improve BER performance, a multi-stage processing can be implemented by cascading multiple stages of the proposed 2D interference cancellation, with a total delay that increases linearly with the number of stages, but independent of the number of users. The BER performance of the single-stage cancellation is analyzed, and the approximated theoretical result well matches the simulation results. Simulations are provided for up-to 3 stages of interference cancellation and compared with the basic TRDMA system without interference cancellation.
A recombinant retroviral system for rapid in vivo analysis of human immunodeficiency virus type 1 susceptibility to reverse transcriptase inhibitors (1997)

We have developed a new recombinant retroviral system in which a library of infectious molecular clones of human immunodeficiency virus type 1 (HIV-1) is constructed with reverse transcriptase (RT) genes derived from viral RNA sequences in plasma. HIV-1 RT is amplified from plasma HIV-1 RNA by nested RT-PCR and cloned into a RT-defective HIV-1 proviral vector (xxLAI-np), generating 10(3) to 10(4) recombinant proviral clones from each reaction. The bulk cloning products or individual molecular clones are transfected into MT-2 cells to generate infectious virus. The resultant viruses are assayed for drug susceptibility in CD4+ cell lines to determine either the dominant phenotype of the recombinant virus mixture or the phenotypes of the individual viral clones. DNA sequencing of the cloned RT genes can identify mutations associated with phenotypic resistance of clonal mixtures or individual clones. This method can be used to rapidly detect the in vivo emergence of HIV-1 quasispecies resistant to RT inhibitors.
Airborne traffic surveillance systems (2004)

Timely information about highway traffic conditions is very important for the Department of Transportation (DOT) and other relevant agencies. Such live information would be very important when traffic incidents or accidents occur. An aerial view is the best for traffic situations, particularly over highways. Unmanned aircraft equipped with video cameras and/or other sensors may be able to deliver the necessary information through video images with relatively low operational costs and risks to human life. ATSS (Airborne Traffic Surveillance System), a project funded by the Florida Department of Transportation, attempts to make this vision a reality. This paper describes how the University of Florida research team implemented a system for ATSS from the ground up, using unmanned aerial vehicles, digital video encoding, and transmission of data and multimedia video streams over FDOT's microwave IP networks.
Structural features in the NH2-terminal region of a model eukaryotic signal peptide influence the site of its cleavage by signal peptidase. (1990)

The 20-amino acid signal peptide of human pre (delta pro)apolipoprotein A-II contains the tripartite domain structure typical of eukaryotic prepeptides, i.e. a positively charged NH2-terminal (n) region, a hydrophobic core (h) region, and a COOH-terminal polar domain (c region). This signal sequence has multiple potential sites for cotranslational processing making it an attractive model for assessing the consequences of systematic structural alterations on the site selected for signal peptidase cleavage. We previously analyzed 40 mutant derivatives of this model preprotein using an in vitro translation/canine microsome processing assay. The results showed that the position of the boundary between the h and c regions and properties of the -1 residue are critical in defining the site of cotranslational cleavage. To investigate whether structural features in the NH2-terminal region of signal peptides play a role in cleavage specificity, we have now inserted various amino acids between the positively charged n region (NH2-Met-Lys) and the h region of a "parental" pre(delta pro)apoA-II mutant that has roughly equal cleavage between Gly18 decreases and Gly20 decreases. Movement of the n/h boundary toward the NH2 terminus results in a dramatic shift in cleavage to Gly18 decreases. Replacement of the Lys2 residue with hydrophilic, negatively charged residues preserves the original sites of cleavage. Replacement with a hydrophobic residue causes cleavage to shift "upstream." Simultaneous alteration of the position of n/h and h/c boundaries has an additive effect on the site of signal peptidase cleavage. None of these mutations produced a marked decrease in the efficiency of in vitro cotranslational translocation or cleavage. However, in sequence contexts having poor signal function, introduction of hydrophobic residues between the n and h regions markedly improved the efficiency of translocation/processing. We conclude that the position of the n/h boundary as well as positioning of the h/c boundary affects the site of cleavage chosen by signal peptidase.
[New therapeutic possibilities with low-affinity NMDA receptor antagonists]. (1996)

Glutamate receptor antagonists with selective action at the N-methyl-D-aspartate (NMDA) receptor are promising agents for the neuroprotective and symptomatic pharmacotherapy of various neuropsychiatric disorders. Although NMDA receptor antagonists of the phencyclidine (PCP) type are precluded from clinical use because of their psychotomimetic properties, amantadine and memantine have been administered to human patients with idiopathic Parkinson's disease and spasticity for many years without serious adverse effects. The mechanisms underlying these differences in psychotogenicity of different NMDA receptor antagonist are currently being discussed. Different affinity to the PCP binding site of the NMDA receptor, region-specific pharmacology, as well as different binding profiles to neurotransmitter receptors other than the NMDA type glutamate receptor, most likely play a role in determining whether an NMDA receptor antagonist drug will be tolerated clinically or not.
Molecular characterization of human IgG monoclonal antibodies specific for the major birch pollen allergen Bet v 1. Anti‐allergen IgG can enhance the anaphylactic reaction (1999)

We report the molecular characterization of five human monoclonal antibodies, BAB1-5 (BAB1: IgG(1); BAB4: IgG(2); BAB2, 3, 5: IgG(4)), with specificity for the major birch pollen allergen, Bet v 1. BAB1-5 were obtained after immunotherapy and contained a high degree of somatic mutations indicative of an antigen-driven affinity maturation process. While BAB1 inhibited the binding of patients IgE to Bet v 1, BAB2 increased IgE recognition of Bet v 1, and, even as Escherichia coli-expressed Fab, augmented Bet v 1-induced immediate type skin reactions. The demonstration that IgG antibodies can enhance allergen-induced allergic reactions is likely to explain the unpredictability of specific immunotherapy.
Coding a terminated bus for low power (2002)

Coding was proposed as a general method of decreasing power dissipation for the I/O. Lower power dissipation can be obtained by using extra bus liner for coding the data. This paper presents an application of the general theory of limited-weight codes for a class of parallel terminated buses with pull-up terminators (e.g. Rambus). Power dissipation on such a bus-line is larger for a logical 1 and it follows that patterns with few 1s should be chosen. A perfect k/2-limited weight code equivalent to the previously proposed Bus-Invert method and a novel non-perfect 3-limited weight code are described. Both codes can be algorithmically generated and practical issues related to their implementation on the Rambus are discussed.
Fast purification of a functional elongator tRNAmetexpressed from a synthetic genein vivo (1988)

Journal Article Fast purification of a functional elongator tRNA met expressed from a synthetic gene in vivo Get access Thierry Meinnel, Thierry Meinnel Laboratoire de Biochiinie, Ecole PolytechniqueUA no. 240 du CNRS, 91128 Palaiseau Cedex, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Yves Mechulam, Yves Mechulam Laboratoire de Biochiinie, Ecole PolytechniqueUA no. 240 du CNRS, 91128 Palaiseau Cedex, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Guy Fayat Guy Fayat Laboratoire de Biochiinie, Ecole PolytechniqueUA no. 240 du CNRS, 91128 Palaiseau Cedex, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 16, Issue 16, 25 August 1988, Pages 8095–8112, https://doi.org/10.1093/nar/16.16.8095 Published: 25 August 1988 Article history Received: 30 June 1988 Accepted: 18 July 1988 Published: 25 August 1988
xylE functions as an efficient reporter gene in Streptomyces spp.: use for the study of galP1, a catabolite-controlled promoter (1989)

We describe the development of a convenient and sensitive reporter gene system for Streptomyces spp. based on the use of a promoterless copy of the xylE gene of Pseudomonas putida. The xylE gene product is a catechol dioxygenase, which converts the colorless substrate catechol to an intensely yellow hydroxymuconic semialdehyde. A promoterless copy of xylE was placed under the transcriptional control of galP1, a glucose-repressed and galactose-induced promoter from Streptomyces lividans, and its expression was examined in bacterial colonies on agar plates or in liquid cultures grown in the presence of glucose or galactose as the sole carbon source. On plates, colonies of bacteria grown on galactose turned bright yellow within a few minutes of being sprayed with a solution of catechol, whereas colonies on glucose-containing plates remained white or only slightly colored, even after extensive incubation. Activity of galP1-xylE fusions was conveniently measured in crude cell extracts with a simple colorimetric assay and was shown to faithfully reflect intracellular RNA levels, as determined by quantitative dot blots. Moreover, differences in expression levels of xylE fusions driven by mutant galP1 promoters were readily apparent in color reactions on plates. The properties of xylE as a reporter gene thus make it suitable not only for quantitatively monitoring expression of regulated promoters in Streptomyces spp. but also for recovering mutations that alter the expression levels of promoters of interest.
Theory of mixed-state effect on NMR relaxation measurements in iron pnictide superconductors (2011)

Based on a phenomenological model with ${s}_{\ifmmode\pm\else\textpm\fi{}}$ or $s$-wave pairing symmetry, the spatially resolved nuclear magnetic resonance (NMR) relaxation rate in the iron pnictides is investigated by solving Bogoliubov-de Gennes equations. Taking into account the presence of a magnetic field, our result for the $s\ifmmode\pm\else\textpm\fi{}$ pairing is in qualitative agreement with recent NMR experiments, while for the $s$-wave pairing, a coherence peak shows up right below ${T}_{c}$ in apparent contradiction with experimental observations, thus excluding the $s$-wave pairing. We also propose that the spin-lattice relaxation rate (SLRR) should follow an exponential relation when the temperature is lowered below $T/{T}_{c}\ensuremath{\approx}0.1$ down to $0.01$. It is noted that the SLRR cannot be entirely determined by the local density of states; the mixed-state effect and multiorbital physics must be considered.
Discovery of 6-Oxo-3-(2-phenylpyrazolo[1,5-a]pyridin-3-yl)-1(6H)-pyridazinebutanoic Acid (FK 838):  A Novel Non-Xanthine Adenosine A1Receptor Antagonist with Potent Diuretic Activity (1999)

ADVERTISEMENT RETURN TO ISSUELetterNEXTDiscovery of 6-Oxo-3-(2-phenylpyrazolo[1,5-a]pyridin-3-yl)-1(6H)-pyridazinebutanoic Acid (FK 838): A Novel Non-Xanthine Adenosine A1 Receptor Antagonist with Potent Diuretic ActivityAtsushi Akahane, Hirohito Katayama, Takafumi Mitsunaga, Takeshi Kato, Takayoshi Kinoshita, Yasuhiro Kita, Takahiro Kusunoki, Takao Terai, Keizo Yoshida, and Youichi ShiokawaView Author Information Medicinal Chemistry Research Laboratories, Fujisawa Pharmaceutical Company, Ltd., 1-6, 2-chome, Kashima, Yodogawa-ku, Osaka 532-8514, Japan Cite this: J. Med. Chem. 1999, 42, 5, 779–783Publication Date (Web):February 20, 1999Publication History Received30 November 1998Published online20 February 1999Published inissue 1 March 1999https://pubs.acs.org/doi/10.1021/jm980671whttps://doi.org/10.1021/jm980671wrapid-communicationACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views669Altmetric-Citations93LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Antagonists,Nucleic acids,Receptors,Rodent models,Solubility Get e-Alerts
The Reaction of Phenylglyoxal with Arginine Residues in Proteins (1968)

The experiments described in this communication were carried out in order to investigate the usefulness of phenylglyoxal as a reagent for the chemical modification of arginine residues in proteins. Phenylglyoxal reacts with the guanido group of the arginine residue under mild conditions, pH 7 to 8 and 25°. The derivative, which contains two phenylglyoxal moieties per guanido group, is sufficiently stable, particularly under mildly acidic conditions (below pH 4) to permit the isolation of labeled peptides. At neutral or alkaline pH values, the derivative is decomposed slowly. About 80% of the original arginine can be regenerated by incubation of the derivative in the absence of excess reagent for 48 hours at 37° and about pH 7. Bovine pancreatic ribonuclease A (0.5% solution) is inactivated to the extent of 80 to 90% within 30 min by reaction with a 1.5% solution of phenylglyoxal at pH 8.0 and 25°. Of the 4 arginine residues in the molecule, 2 to 3 are modified. Analysis of tryptic-chymotryptic hydrolysates of the inactivated protein show that condensation occurs primarily at arginine residues 39 and 85 in the sequence. These are the 2 arginine residues which x-ray diffraction studies have shown to be closest to the active site of the enzyme. In this modified ribonuclease, the 2 histidine residues, 12 and 119, continue to be susceptible to specific alkylation by iodoacetate. In general, phenylglyoxal (14C-labeled, if desired) may be useful for modification of accessible arginine residues in proteins and may be potentially useful for reversible coverage of arginine residues so that tryptic hydrolysis can take place at lysine residues only. When proteins are treated with a large excess of the reagent for a long period, reaction may also occur with e-amino groups of lysine residues. In all cases, any available α-amino group is deaminated to give a residue of the corresponding α-keto acid. The reagent is very effective in deaminating small peptides to the corresponding α-keto acyl peptides, thus permitting the identification of the end groups by subtractive analysis.