gebhart/patrepeval huggingface dataset:
Wound Healing and Angiogenic Properties of Supernatants from Lactobacillus Cultures (2003)

Extracts or supernatants from cultures of Lactobacilli are used for their medicinal effects, including wound healing and immune system stimulating activity. We have studied the in vivo and in vitro effects of supernatants from bacterial cultures of two strains of Lactobacillus (LS) on tissue repair and angiogenesis. Subcutaneous injection of LS into rodent ears led to proliferation of blood vessels that also exhibited strong immunostaining for Flk-1 receptor. Some inflammatory cells were scattered among the blood vessels. The continuous influx of polymorphonuclear leukocytes (PMNs) and macrophages into transcutaneous wounds in mice treated with LS resulted in prolonged inflammatory phase of wound healing and delayed wound closure, including reepithelialization. Subcutaneous injection of Matrigel impregnated with LS into the abdominal wall led to rapid and transient influx of PMNs in the vicinity of the gel. LS stimulated the proliferation of murine macrophage J774.A1 cell line and porcine lymphocytes but not that of murine fibroblast AKR-2B cells. LS also induced production of TNF-alpha by J774.A1 cells and by porcine kidney epithelial LLC-PK1 cells. LS did not appear to have an effect on collagen production. In conclusion, our study demonstrates the potential of LS to function as a stimulator of the inflammatory stage of tissue repair, TNF-alpha production, and of angiogenesis.
Nuclear factor-κB is constitutively activated in primitive human acute myelogenous leukemia cells (2001)

Human acute myelogenous leukemia (AML) is thought to arise from a rare population of malignant stem cells. Cells of this nature, herein referred to as leukemic stem cells (LSCs), have been documented for nearly all AML subtypes and appear to fulfill the criteria for stem cells in that they are self-renewing and give rise to the cells found in many leukemic populations. Because these cells are likely to be critical for the genesis and perpetuation of leukemic disease, the present studies sought to characterize unique molecular properties of the LSC population, with particular emphasis on the transcription factor, nuclear factor-kappaB (NF-kappaB). Previous experiments have shown that unstimulated human CD34(+) progenitor cells do not express NF-kappaB. In contrast, primary AML CD34(+) cells display readily detectable NF-kappaB activity as assessed by electrophoretic mobility shift assay and gene expression studies. Furthermore, detailed analyses of enriched AML stem cells (CD34(+)/CD38(-)/CD123(+)) indicate that NF-kappaB is also active in the LSC population. Given the expression of NF-kappaB in leukemic, but not normal primitive cells, the hypothesis that inhibition of NF-kappaB might induce leukemia-specific apoptosis was tested by treating primary cells with the proteasome inhibitor MG-132, a well-known inhibitor of NF-kappaB. Leukemic CD34(+)/CD38(-) cells displayed a rapid induction of cell death in response to MG-132, whereas normal CD34(+)/CD38(-) cells showed little if any effect. Taken together, these data indicate that primitive AML cells aberrantly express NF-kappaB and that the presence of this factor may provide unique opportunities to preferentially ablate LSCs.
Self-Similar Chain of Metal Nanospheres as an Efficient Nanolens (2003)

As an efficient nanolens, we propose a self-similar linear chain of several metal nanospheres with progressively decreasing sizes and separations. To describe such systems, we develop the multipole spectral expansion method. Optically excited, such a nanolens develops the nanofocus ("hottest spot") in the gap between the smallest nanospheres, where the local fields are enhanced by orders of magnitude due to the multiplicative, cascade effect of its geometry and high Q factor of the surface plasmon resonance. The spectral maximum of the enhancement is in the near-ultraviolet region, shifting toward the red region as the separation between the spheres decreases. The proposed system can be used for nanooptical detection, Raman characterization, nonlinear spectroscopy, nanomanipulation of single molecules or nanoparticles, and other applications.
Different Anomeric Sugar Bound States of Maltose Binding Protein Resolved by a Cytolysin A Nanopore Tweezer (2020)

Conformational changes of proteins are essential to their functions. Yet it remains challenging to measure the amplitudes and time scales of protein motions. Here we show that the cytolysin A (ClyA) nanopore was used as a molecular tweezer to trap a single maltose-binding protein (MBP) within its lumen, which allows conformation changes to be monitored as electrical current fluctuations in real time. In contrast to the current two state binding model, the current measurements revealed three distinct ligand-bound states for MBP in the presence of reducing saccharides. Our analysis reveals that these three states represented MBP bound to different isomers of reducing sugars. These findings contribute to the understanding of the mechanism of substrate recognition by MBP and illustrate that the nanopore tweezer is a powerful, label-free, single-molecule approach for studying protein conformational dynamics under functional conditions.
Noninvasive in vivo glucose sensing on human subjects using mid-infrared light (2014)

Mid-infrared quantum cascade laser spectroscopy is used to noninvasively predict blood glucose concentrations of three healthy human subjects in vivo. We utilize a hollow-core fiber based optical setup for light delivery and collection along with a broadly tunable quantum cascade laser to obtain spectra from human subjects and use standard chemo-metric techniques (namely partial least squares regression) for prediction analysis. Throughout a glucose concentration range of 80-160 mg/dL, we achieve clinically accurate predictions 84% of the time, on average. This work opens a new path to a noninvasive in vivo glucose sensor that would benefit the lives of hundreds of millions of diabetics worldwide.
A hierarchical and topological classification of linear sensor networks (2009)

Considerable advancements in the technology of wireless sensor networks (WSNs) are taking place due to smaller, and more efficient electronic devices which are capable of increased processing power, and communication capabilities. In addition, the cost of such devices is constantly decreasing, which makes it possible to employ large quantities of networked sensors in numerous commercial, environmental, military and health care applications. A lot of these applications involve lining up the sensor nodes in a linear structure giving rise to a new class of WSNs, which is defined in this work as Linear Sensor Networks (LSNs). This paper identifies some of the applications that might use such networks and offers a classification of the different types of LSNs from a topological and hierarchical points of view. Finally, a motivation for designing specialized protocols that take advantage of the linearity of such networks in order to increase reliability, efficiency, energy savings, and network lifetime is offered along with the new research issues, challenges and opportunities that exist in this field.
Straighforward in-line processing for a 16.8% efficient mc-Si solar cell (2005)

A simple in-line industrial process has been developed for commercial multicrystalline silicon (mc-Si) which results in solar cells with an average efficiency of 16.5%. The best cell has an efficiency of 16.8%. These are the highest efficiencies reported for full inline processing. The process consists of an acidic etch for texturing, homogeneous spin-on phosphorous and a belt furnace emitter diffusion, MicroWave PECVD for silicon nitride layers, and screen-printed metallization. The silicon nitride layer serves as antireflection coating and provides bulk and surface passivation. Detailed characterization and computer simulation showed that implementation of already proven technologies in the current cell processing could lead to efficiencies close to 18%.
GENETICS OF CULTIVATED CUCURBITS (1944)

Journal Article GENETICS OF CULTIVATED CUCURBITS Get access C. F. POOLE C. F. POOLE *Cytologist, Division of Fruit and Vegetable Crops and Diseases, Bureau of Plant Industry, United States Department of Agriculture. Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Heredity, Volume 35, Issue 4, April 1944, Pages 122–128, https://doi.org/10.1093/oxfordjournals.jhered.a105364 Published: 01 April 1944
[LAV type II: a second retrovirus associated with AIDS in West Africa]. (1986)

A second retrovirus, named LAV-II, has been isolated from two West-African patients with AIDS. By its genomic sequences and its proteins, this virus is different from the LAV-I/HTLV-III, isolated from U.S.A., Europe and Central Africa. It differs also from STLV-III, isolated from Rhesus Macaques with AIDS, but displays an antigenic relationship with the latter virus, at the level of its external envelope protein. The tropism of LAV-II for T4 lymphocytes and the induction of cytopathic effect in infected cells are similar to those of LAV-I.
Analysis of B-Scan Speckle Reduction by Resolution Limited Filtering (1982)

Much of the signal energy due to speckle in ultrasound images is shown to be of higher spatial frequency than the intrinsic pulse shape limited resolution in B-scan ultrasound images. A significant increase in signal to noise ratio can therefore be obtained by resolution limited spatial filtering which selectively removes energy of higher spatial frequency than the pulse envelope resolution limit. The concept is illustrated by resolution limited filtering ECG gated B-scan echocardiographic images. Signal to noise improvement is illustrated by comparing time-motion displays generated from both processed and unprocessed images.
Detection of Generalized-Roughness Bearing Fault by Spectral-Kurtosis Energy of Vibration or Current Signals (2009)

Generalized roughness is the most common damage occurring to rolling bearings. It produces a frequency spreading of the characteristic fault frequencies, thus making it difficult to detect with spectral or envelope analysis. A statistical analysis of typical bearing faults is proposed here in order to identify the spreading bandwidth related to specific conditions, relying on current or vibration measurements only. Then, a diagnostic index based on the computation of the energy in the previously defined bandwidth is used to diagnose bearing faults. The proposed method was validated experimentally with vibration signals, with robust and reliable results. The same procedure can be extended to current signals.
Efficient Transformation of Azides to Primary Amines Using the Mild and Easily Accessible CeCl3·7H2O/NaI System (2008)

Because of the nitrogen functionality, the azido group plays an important role in the synthesis of amines, and numerous reduction methods of azides to primary amines are reported. Recent reports have highlighted the capability of NaI as a useful reagent for this transformation when it is used in combination with a Lewis acid promoter. However, these methods often suffer from harsh reaction conditions; for this reason, the development of a simple and efficient protocol using NaI in presence of inexpensive and readily available cerium salts Lewis acids would extend the scope of this organic transformation. In continuation of our interest on the use of the CeCl3.7H2O/NaI system, in this paper we report how azides undergo reduction by NaI in the presence of CeCl3.7H2O in refluxing acetonitrile under neutral conditions to produce the corresponding primary amines. The rate and yield of the reaction are considerably improved by employing this microwave-assisted procedure, and this may be of value for the preparation of densely functionalized molecules having biological and pharmaceutical activities.
The Relationship between Quadriceps Muscle Force, Knee Flexion, and Anterior Cruciate Ligament Strain in an in Vitro Simulated Jump Landing (2005)

These results suggest that even in the presence of knee flexor muscle forces, the increase in quadriceps force required to prevent the knee from flexing during landing can place the anterior cruciate ligament at risk for large strains.
Series Introduction: Stem cells near the century mark (2000)

The concept of a stem cell, now nearing its hundredth year as one of the organizing principles of developmental biology, shows no sign of losing its youthful luster. At a time when the transfer of biological concepts to clinical practice drives much of science, the properties of stem cells in various tissues are attracting increasing levels of interest. This attention is not restricted to the germ line and blood, the traditional domain of stem cells as defined by early 20th-century studies on animal development, but has been extended to tissues not typically thought to turn over. As novel sites, properties, and functions have been identified for these cells, the definition of a stem cell has shifted repeatedly. Like some stem cells, this concept has expanded greatly and has displayed a remarkable degree of plasticity.
Associations between branched chain amino acid intake and biomarkers of adiposity and cardiometabolic health independent of genetic factors: A twin study (2016)

Higher intakes of BCAA were associated, independently of genetics, with lower insulin resistance, inflammation, blood pressure and adiposity-related metabolites. The BCAA intake associated with our findings is easily achievable in the habitual diet.
A simplified protocol for fast plasmid DNA sequencing (1990)

Journal Article A simplified protocol for fast plasmid DNA sequencing Get access J. Zimmermann, J. Zimmermann European Molecular Biology LaboratoryPostfach 102209, 6900 Heidelberg, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar H. Voss, H. Voss European Molecular Biology LaboratoryPostfach 102209, 6900 Heidelberg, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar C. Schwager, C. Schwager European Molecular Biology LaboratoryPostfach 102209, 6900 Heidelberg, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar J. Stegemann, J. Stegemann European Molecular Biology LaboratoryPostfach 102209, 6900 Heidelberg, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar H. Erfle, H. Erfle European Molecular Biology LaboratoryPostfach 102209, 6900 Heidelberg, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar K. Stucky, K. Stucky European Molecular Biology LaboratoryPostfach 102209, 6900 Heidelberg, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar T. Kristensen, T. Kristensen European Molecular Biology LaboratoryPostfach 102209, 6900 Heidelberg, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar W. Ansorge W. Ansorge * European Molecular Biology LaboratoryPostfach 102209, 6900 Heidelberg, FRG *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 18, Issue 4, 25 February 1990, Page 1067, https://doi.org/10.1093/nar/18.4.1067 Published: 25 February 1990 Article history Received: 19 January 1990 Published: 25 February 1990
Natural Language Processing in Medicine: An Overview (1996)

An overview is given of natural language processing applications in medicine. An attempt has been made to enumerate the most important and known international projects and to summarize their goals, principles, methods and results. A section is devoted to projects involving the Dutch language. A more general discussion about the two fundamental approaches concerning medical language understanding is provided. An extensive bibliography may be useful for those wishing to explore this research domain.
Phosphorylcholine on the Lipopolysaccharide of Haemophilus influenzae Contributes to Persistence in the Respiratory Tract and Sensitivity to Serum Killing Mediated by C-reactive Protein (1998)

Haemophilus influenzae undergoes phase variation in expression of the phosphorylcholine (ChoP) epitope, a structure present on several invasive pathogens residing in the human respiratory tract. In this study, structural analysis comparing organisms with and without this epitope confirmed that variants differ in the presence of ChoP on the cell surface-exposed outer core of the lipopolysaccharide. During nasopharyngeal carriage in infant rats, there was a gradual selection for H. influenzae variants that express ChoP. In addition, genotypic analysis of the molecular switch that controls phase variation predicted that the ChoP+ phenotype was predominant in H. influenzae in human respiratory tract secretions. However, ChoP+ variants of nontypable H. influenzae were more sensitive to the bactericidal activity of human serum unrelated to the presence of naturally acquired antibody to ChoP. Serum bactericidal activity required the binding of C-reactive protein (CRP) with subsequent activation of complement through the classical pathway. Results of this study suggested that the ability of H. influenzae to vary expression of this unusual bacterial structure may correlate with its ability both to persist on the mucosal surface (ChoP+ phenotype) and to cause invasive infection by evading innate immunity mediated by CRP (ChoP- phenotype).
Efficacy and safety of lebrikizumab (an anti-IL-13 monoclonal antibody) in adults with moderate-to-severe atopic dermatitis inadequately controlled by topical corticosteroids: A randomized, placebo-controlled phase II trial (TREBLE) (2018)

When combined with TCS, lebrikizumab 125 mg taken every 4 weeks led to a significant improvement and was well tolerated in patients with moderate-to-severe AD.
Identification of a Novel 27‐kDa Protein from Mycobacterium tuberculosis Culture Fluid by a Monoclonal Antibody Specific for the Mycobacterium tuberculosis Complex (1993)

Mycobacterium tuberculosis antigens inducing species-specific immune responses are likely to be particularly important for serodiagnosis or for skin testing of tuberculosis. In the present study, we describe the characterization of two novel monoclonal antibodies (MoAbs) A3h4 (IgG2a) and B5g1 (IgM) that are directed to M. tuberculosis 27-kDa and 25-kDa proteins respectively. Specificity analysis by immunoblotting using 20 different species of mycobacterial sonicates revealed that MoAb A3h4 was specific for M. tuberculosis complex alone while MoAb B5g1 showed a limited cross-reactivity. Direct comparison with previously characterized MoAbs revealed that these MoAbs A3h4 and B5g1 defined new antigenic determinants of M. tuberculosis. By using M. tuberculosis complex-specific MoAb A3h4 we have identified a distinct 27-kDa protein in the M. tuberculosis H37Rv culture fluid. Since this MoAb did not bind to the previously characterized MPT44, MPT59, MPT45, MPT51 and MPT64 proteins as well as the 23-kDa superoxide dismutase (SOD) protein of M. tuberculosis, we conclude that MoAb A3h4 recognizes a novel protein in the M. tuberculosis H37Rv culture fluid. Studies of the subcellular distribution of these MoAb-reactive proteins indicate that the MoAb A3h4-reactive 27-kDa protein is present not only in the culture fluid but also in the cytosol and the cell wall of M. tuberculosis. By contrast, B5g1-reactive protein is mainly a cytosolic protein. When these MoAbs were tested in a previously established ELISA with intact mycobacteria derived from early cultures, only MoAb A3h4 showed the positive reactivity to mycobacteria belonging to the M. tuberculosis complex. In addition, during the present comparative studies of MoAbs we have also found that the previously described MoAb F116-5, which is known to recognize the mycobacterial 23-kDa SOD protein [17], cross-reacted with the MPT44, MPT59, MPT45 and MPT51 secreted proteins but not with MPT64 and MPB70. These findings indicate that the family of four secreted proteins of M. tuberculosis share a common epitope with M. tuberculosis SOD protein.
Strain and species identification by restriction fragment length polymorphisms in the ribosomal DNA repeat of Candida species (1987)

Restriction fragment length polymorphisms in the ribosomal DNA (rDNA) have been shown to be a useful criterion for distinguishing among various isolates of Candida albicans. In a sample of 12 clinical isolates, we found six different classes based on variations in the fragments produced from genomic DNA by EcoRI and visualized after Southern transfer by being probed with a plasmid containing Saccharomyces cerevisiae rDNA. Some of the classes appeared to be heterozygous at the rDNA locus. Similar digestion of other Candida species showed that each could be identified on the basis of its restriction patterns. Since these are highly reiterated genes, the differences were apparent on ethidium bromide-stained gels; Southern transfers were not necessary. EcoRI restriction maps of the rDNA of C. albicans, C. stellatoidea, C. tropicalis, and C. guilliermondii were determined.
Uterine Papillary Serous Carcinoma (2011)

Uterine papillary serous carcinoma (UPSC) is a histologic variant of endometrial cancer that accounts for only 10% of new cases of uterine cancer but is responsible for 40% of deaths from the disease. UPSC is an aggressive tumor with a predilection for early spread beyond the uterus. Treatment for UPSC typically entails surgery and in most women is followed by multimodality adjuvant therapy. In this review, we describe the epidemiology, natural history, treatment, and outcome of UPSC.
Autoantigenicity of Human Sperm: Molecular Identities of Sperm Antigens Recognized by Sera of Immunoinfertile Men in the Immunoprecipitation Procedure (1995)

Autoantigenicity of human sperm as related to immunoinfertility was investigated using the immunoprecipitation procedure. Sera from immunoinfertile men specifically immunoprecipitated six specific bands of 135, 95, and 65 kD and double bands of 47, 41, and 23 kD that were not recognized by the sera from fertile men. Of these six specific bands, three bands of 65-, 47-, and 23-kD molecular identities were recognized by > 60% of immunoinfertile sera, and 47 and 23 kD may correspond to the dimeric and monomeric forms of FA-1 antigen, respectively. Seminal plasma from immunoinfertile men specifically recognized three specific bands of 95, 37, and 16 kD; only the 95-kD band was also recognized with the immunoinfertile sera. These specific antigens may find applications in immunocontraception and immunoinfertility in humans.
Comparative Analysis of the Effect of Aminoglycosides on Bacterial Protein Synthesis in vitro (1979)

The effect of aminoglycoside antibiotics on bacterial protein synthesis in an initiating system was investigated. Three classes of compounds can be differentiated as to their response. (1) Antibiotics (streptomycin, bluensomycin and hygromycin B) which inhibit polypeptide synthesis in a monophasic way indicating a single major inhibitory site. Polypeptides made under partially inhibitory conditions possess the same size as those produced without the drug. (2) Compounds containing 2‐deoxystreptamine in a diglycosylated form (gentamicins B, C 1 , C 1a and C 2 ; sisomicin; kanamycin; neomycin; tobramycin; lividomycin A and paromomycin) inhibit protein synthesis in the triphasic way recently reported for gentamicin C complex which indicates multiple interactions of these antibiotics with the ribosome. These compounds also share the property of promoting the synthesis of longer than normal peptides due to termination errors. (3) The disaccharidic sisomicin and lividomycin cleavage products, garamine and lividamine respectively, give a biphasic response, namely stimulation of the rate of polypeptide synthesis at low concentration and inhibition at increasing concentrations. Aminoglycosides of class 2 (when present at second‐phase concentration) can revert the inhibition of protein synthesis caused by each of the class 1 compounds. Concomitantly, ‘read‐through’ products are accumulated.