gebhart/patrepeval huggingface dataset:
Stimulation of the Ventral Subiculum of the Hippocampus Evokes Glutamate Receptor‐mediated Changes in Dopamine Efflux in the Rat Nucleus Accumbens (1997)

The effects of electrical stimulation of the ventral subiculum/CA1 region of the hippocampus on changes in dopamine oxidation current (corresponding to dopamine efflux) in the nucleus accumbens were examined using in vivo chronoamperometry with stearate-graphite paste electrodes in urethane-anaesthetized rats. Burst-patterned monophasic pulses (10-100 Hz/burst delivered at 0.8-4 Hz) evoked a three-component change in dopamine efflux in the nucleus accumbens with an initial transient increase in the dopamine signal above baseline, followed by an immediate decrease below baseline, and thereafter by a prolonged increase in the dopamine signal above baseline. 6-Hydroxydopamine lesions of the mesoaccumbens dopamine pathway or transection of the fimbria-fornix blocked all of the evoked changes in the dopamine signal. Both the first and third components of enhanced dopamine efflux were blocked by microinfusion into the nucleus accumbens of the ionotropic glutamate receptor antagonists (+/-)-2-amino-5-phosphonopentanoic acid, 6,7-dinitroquinoxaline-2,3-dione and kynurenate. Burst stimulation-evoked decreases in the dopamine signal were abolished following microinfusions into the nucleus accumbens of the metabotropic glutamate receptor antagonist (+)-alpha-methyl-4-carboxyphenylglycine. These results suggest that ventral subiculum/CA1 glutamatergic inputs to the nucleus accumbens may presynaptically modulate dopamine efflux by synaptic activation of both ionotropic and metabotropic glutamate receptors in the nucleus accumbens. These glutamate-dopamine interactions may constitute part of the mechanisms by which hippocampal signals are integrated through selective modulation of dopamine release in the nucleus accumbens in both physiological and pathological conditions.
Effects of dry heat and saturated steam on the physical properties of chitosan (1999)

Heat may be employed to facilitate the processing of chitosan and to confer sterility on chitosan-based medical products. In this study, changes were analyzed of the physical properties of purified chitosan heated at 60 to 160°C under specified conditions for periods ranging from 0.5 to 4 h. Two forms of heat were used for processing: dry heat generated by a convection oven and saturated steam generated by an autoclave. Dry heat at ≤80°C resulted in less rigid chains with lower glass transition temperature, improved aqueous solubility, and slightly higher [η]. At higher temperatures, dry heat produced chromophores, which may be related to interchain crosslink formation involving the NH2 groups. The [η] and aqueous solubility of the samples decreased with temperatures ≥120°C. The coloration of the samples intensified from yellow to brown with increasing temperatures and duration of heat exposure. Chitosan heated at 160°C for ≥2 h was insoluble in the 0.2 M acetic acid/0.1 M sodium acetate solvent. The rate and extent of the thermal reactions were increased in the presence of saturated steam; the autoclaved samples became insoluble after 2 h of heating at 115°C and after 1 h at ≥120°C. On the other hand, the physical changes induced by dry heat at ≤120°C were not affected significantly by heating the chitosan samples under anoxic conditions. © 1999 John Wiley & Sons, Inc. J Biomed Mater Res (Appl Biomater) 48: 111–116, 1999
Reverse transcriptase of mouse mammary tumour virus: expression in bacteria, purification and biochemical characterization (1998)

We have constructed a plasmid that induces in bacteria the synthesis of an enzymically active reverse transcriptase (RT) of mouse mammary tumour virus (MMTV), a retrovirus with a typical B-type morphology. The highest catalytic activity was detected only when 27 residues from the C-terminus of the protease were included in the N-terminus of the recombinant RT, after an extra deoxyadenosine was added between the pro and pol genes to overcome the -1 frameshift event (which occurs naturally in virus-infected cells). The recombinant protein with a six-histidine tag was purified to homogeneity by a two-column purification procedure, Ni2+ nitriloacetic acid/agarose followed by carboxymethyl-Sepharose chromatography. Unlike most RTs, the purified MMTV RT is enzymically active as a monomer even after binding a DNA substrate. Like all RTs studied, the recombinant MMTV RT possesses RNA-dependent and DNA-dependent DNA polymerase activities as well as RNase H activity, all of which show a preference for Mg2+ over Mn2+ ions. Other features of these enzymic activities, such as extension of DNA primers, processivity of DNA synthesis, pH dependence, steady-state kinetic constants, effects of Na+ or K+ ions and sensitivity to a thiol-specific reagent and to a zinc chelator, have been evaluated. The catalytic properties of MMTV RT were compared with those of the well-studied RT of HIV-1, the causative agent of AIDS. Interestingly, MMTV RT exhibits a high sensitivity to nucleoside triphosphate analogues (which are known to be potent inhibitors of HIV RTs and are being used as the major anti-AIDS drugs), as high as that of HIV-1 and HIV-2 RTs. Furthermore the recombinant MMTV RT shows a processivity of DNA synthesis higher than that of HIV-1 RT.
Mapping of the human gene for epidermal growth factor receptor (EGFR) on the p13→q22 region of chromosome 7 (1983)

We previously reported that the structural gene for epidermal growth factor receptor (EGFR) can be mapped to the p22 leads to qter region of human chromosome 7 (Shimizu et al., 1979, 1980). In the present study, we produced two series of human-mouse cell hybrids by fusing mouse A9 cells that are deficient in EGFR with the human diploid fibroblast lines GM1356, 46,XX,t(1;7)(p34;p13), and GM2068, 46,XX,t(6;7)(q27;q22), both of which possess EGF receptors. Expression of EGF binding ability in the former series of cell hybrids was correlated with the retention of the human translocation chromosome containing the 7p13 leads to qter region, and in the latter series of cell hybrid it was correlated with the retention of the human translocation chromosome containing the 7pter leads to q22 region. Therefore, the EGFR gene can be localized in the p13 leads to q22 region of chromosome 7.
Ultrahigh-resolution, high-speed, Fourier domain optical coherence tomography and methods for dispersion compensation (2004)

Ultrahigh-resolution optical coherence tomography uses broadband light sources to achieve axial image resolutions on the few micron scale. Fourier domain detection methods enable more than an order of magnitude increase in imaging speed and sensitivity, thus overcoming the sensitivity limitations inherent in ultrahigh-resolution OCT using standard time domain detection. Fourier domain methods also provide direct access to the spectrum of the optical signal. This enables automatic numerical dispersion compensation, a key factor in achieving ultrahigh image resolutions. We present ultrahigh-resolution, high-speed Fourier domain OCT imaging with an axial resolution of 2.1 ìm in tissue and 16,000 axial scans per second at 1024 pixels per axial scan. Ultrahigh-resolution spectral domain OCT is shown to provide a ~100x increase in imaging speed when compared to ultrahigh-resolution time domain OCT. In vivo imaging of the human retina is demonstrated. We also present a general technique for automatic numerical dispersion compensation, which is applicable to spectral domain as well as swept source embodiments of Fourier domain OCT.
Interferon induces proliferation in leukemic and normal B‐cell subsets (1986)

3H-thymidine incorporation following stimulation with interferon (IFN) in vitro was investigated in cell cultures from peripheral blood of patients with chronic lymphocytic leukemia (CLL), spleens from necro-kidney transplants and healthy blood donors. It was demonstrated, that IFN can induce a proliferative response in some normal as well as leukemic B lymphocyte subsets. The responses were not T-cell dependent. The results indicate, that B-cell subsets that proliferate in the presence of IFN, are present in higher proportions in spleen than in peripheral blood, and that they constitute a portion of the leukemic blood lymphocyte pool in some patients with CLL. We have previously demonstrated, that IFN induces varying degrees of transformation and differentiation in blood lymphocytes from a majority of CLL patients. The functional characteristics of different B-cell subsets, and their heterogeneous distribution in leukemia, may be important for the results of IFN treatment in various malignant B-cell disorders.
A review of fMRI studies during visual emotive processing in major depressive disorder (2014)

In general, depressed patients have increased activation to emotive, especially negative, visual stimuli in regions involved in affective processing, with the exception of certain PFC regions; this pattern tends to normalize with treatment.
[Tumor markers. Personal experience--screening of breast cancer by determining CEA in nipple discharge]. (1991)

Secondary prevention, detection and treatment at an early stage, may be the only means of controlling breast cancer. This is rational behind screening for breast cancer. We have previously reported that CEA measurement in nipple discharge is a useful adjunct in the diagnosis of nonpalpable breast cancer. As an extension, a dot-immunobinding assay was developed to screen a large number of patients with nipple discharge for nonpalpable breast cancer. This article is a review of the current status of CEA assay in nipple discharge for mass screening of breast cancer. False positive and negative cases will be also described.
Detection of differentially expressed genes in primary tumor tissues using representational differences analysis coupled to microarray hybridization (1998)

The identification of differential gene expressionbetween cells is a frequent goal in modern biological research. Here we demonstrate the coupling of representational difference analysis (RDA) of cDNA with microarray analysis of the output for high throughput screening. Two primary Ewing's sarcoma tissue samples with different biological behavior in vivo were compared by RDA: one which was metastatic and progressed rapidly; the other localized and successfully treated. A modified RDA protocol that minimizes the necessary starting material was employed. After a reduced number of subtractive rounds, the output of RDA was shotgun cloned into a plasmid vector. Inserts from individual colonies from the subtracted library were amplified with vector-specific primers and arrayed at high density on glass slides. The arrays were then hybridized with differentially fluorescently labeled starting amplicons from the two tissues and fluorescent signals were measured at each DNA spot. We show that the relative amounts of fluorescent signal correlate well with the abundance of fragments in the RDA amplicon and in the starting mRNA. In our system, we analyzed 192 products and 173 (90%) were appropriately detected as being >2-fold differentially expressed. Fifty unique, differentially expressed clones were identified. Therefore, the use of RDA essentially provides an enriched library of differentially expressed genes, while analysis of this library with microarrays allows rapid and reproducible screening of thousands of DNA molecules simultaneously. The coupling of these two techniques in this system resulted in a large pool of differentially expressed genes.
A Faster Software Implementation of the Supersingular Isogeny Diffie-Hellman Key Exchange Protocol (2017)

Since its introduction by Jao and De Feo in 2011, the supersingular isogeny Diffie-Hellman (SIDH) key exchange protocol has positioned itself as a promising candidate for post-quantum cryptography. One salient feature of the SIDH protocol is that it requires exceptionally short key sizes. However, the latency associated to SIDH is higher than the ones reported for other post-quantum cryptosystem proposals. Aiming to accelerate the SIDH runtime performance, we present in this work several algorithmic optimizations targeting both elliptic-curve and field arithmetic operations. We introduce in the context of the SIDH protocol a more efficient approach for calculating the elliptic curve operation <inline-formula><tex-math notation="LaTeX">$P+[k]Q$</tex-math></inline-formula> . Our strategy achieves a factor 1.4 speedup compared with the popular variable-three-point ladder algorithm regularly used in the SIDH shared secret phase. Moreover, profiting from pre-computation techniques our algorithm yields a factor 1.7 acceleration for the computation of this operation in the SIDH key generation phase. We also present an optimized evaluation of the point tripling formula, and discuss several algorithmic and implementation techniques that lead to faster field arithmetic computations. A software implementation of the above improvements on an Intel Skylake Core i7-6700 processor gives a factor 1.33 speedup against the state-of-the-art software implementation of the SIDH protocol reported by Costello-Longa-Naehrig in CRYPTO 2016.
Novel antimicrobial peptides derived from human immunodeficiency virus type 1 and other lentivirus transmembrane proteins (1997)

We have previously described a conserved set of peptides derived from lentiviral envelope transmembrane proteins that are similar to the natural antimicrobial peptides cecropins and magainins in overall structure but bear no sequence homology to them or other members of their class. We describe here an evaluation of the antimicrobial properties of these virally derived peptides, designated lentivirus lytic peptides (LLPs). The results of this study demonstrate that they are potent and selective antibacterial peptides: the prototype sequence, LLP1, is bactericidal to both gram-positive and gram-negative organisms at micromolar concentrations in 10 mM phosphate buffer. Furthermore, LLP1 kills bacteria quite rapidly, causing a 1,000-fold reduction in viable organisms within 50 s. Peptides corresponding to sequences from three lentivirus envelope proteins were synthesized and characterized. Several of these peptides are selective, killing bacteria at concentrations 50- to 100-fold lower than those required to lyse erythrocytes. Development of antimicrobial agents based on these peptides may lead to improved therapeutics for the management of a variety of infectious diseases.
Human CD8 transgene regulation of HLA recognition by murine T cells. (1995)

A series of human CD8 transgenic (hCD8 Tg) mice with differential expression in the thymus and periphery were produced to investigate CD8 coreceptor regulation of repertoire selection and T cell responses. Expression of hCD8 markedly enhanced responses to both HLA class I molecules and hybrid A2/Kb molecules providing functional evidence for a second interaction site, outside of the alpha 3 domain, which is essential for optimal coreceptor function. Peripheral T cell expression of hCD8 was sufficient to augment responsiveness to HLA class I, as hCD8 Tg mice which lacked thymic expression responded as well as mice expressing hCD8 in the thymus and periphery. Both murine CD8+ and CD4+ T cells expressing hCD8 transgenes exhibited markedly enhanced responses to foreign HLA class I, revealing the ability of T cell receptor repertoires selected on either murine class I or class II to recognize human class I major histocompatibility complex (MHC). In contrast to recognition of foreign class I, thymic expression of hCD8 transgenes was absolutely required to enhance recognition of antigenic peptide restricted by self-HLA class I. Thus, our studies revealed disparate requirements for CD8 coreceptor expression in the thymus for selection of a T cell repertoire responsive to foreign MHC and to antigenic peptides bound to self-MHC, providing a novel demonstration of positive selection that is dependent on human CD8.
A Practical Differentially Private Random Decision Tree Classifier (2009)

In this paper, we study the problem of constructing private classifiers using decision trees, within the framework of differential privacy. We first construct privacy-preserving ID3 decision trees using differentially private sum queries. Our experiments show that for many data sets a reasonable privacy guarantee can only be obtained via this method at a steep cost of accuracy in predictions. We then present a differentially private decision tree ensemble algorithm using the random decision tree approach. We demonstrate experimentally that our approach yields good prediction accuracy even when the size of the datasets is small. We also present a differentially private algorithm for the situation in which new data is periodically appended to an existing database. Our experiments show that our differentially private random decision tree classifier handles data updates in a way that maintains the same level of privacy guarantee.
On the theory of self-diffusion in a polymer gel (1984)

The problem of self-diffusion in a random medium consisting of a solvent and a fixed set of scattering centers (obstacles) is discussed. A new equation describing diffusion of a Brownian particle in such a system is derived. We find that the self-diffraction coefficient of the particle depends on the concentration of scattering centers like D/D0=1−A√C0−BC0+⋅⋅⋅, where A and B are positive constants, depending on the size of particle and repulsive range of scatterers, and D0 is the diffusion coefficient in a pure solvent.
Hybrid tracking for outdoor augmented reality applications (2002)

We've developed a fully mobile, wearable AR system that combines a vision-based tracker (primarily software algorithms) that uses natural landmarks, with an inertial tracker (custom hardware and firmware) based on silicon micromachined accelerometers and gyroscopes. Unlike other vision-based and hybrid systems, both components recover the full 6 DOF pose. Fusing the two tracking subsystems gives us the benefits of both technologies, while the sensors' complementary nature helps overcome sensor-specific deficiencies. Our system is tailored to affordable, lightweight, energy-efficient mobile AR applications for urban environments, especially the historic centers of European cities.
Nile Red as a solvatochromic dye for measuring solvent strength in normal liquids and mixtures of normal liquids with supercritical and near critical fluids (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNile Red as a solvatochromic dye for measuring solvent strength in normal liquids and mixtures of normal liquids with supercritical and near critical fluidsJerry F. Deye, T. A. Berger, and Albert G. AndersonCite this: Anal. Chem. 1990, 62, 6, 615–622Publication Date (Print):March 15, 1990Publication History Published online1 May 2002Published inissue 15 March 1990https://pubs.acs.org/doi/10.1021/ac00205a015https://doi.org/10.1021/ac00205a015research-articleACS PublicationsRequest reuse permissionsArticle Views3252Altmetric-Citations309LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Effects of aging on the immunopathologic response to sepsis (2009)

Aged mice are more likely to die of sepsis than young mice when subjected to an equivalent insult, and this is associated with increases in both systemic and local inflammation. There is an exaggerated local but not systemic inflammatory response in aged mice compared with young mice when mortality is similar. This suggests that systemic processes that culminate in death may be age independent, but the local inflammatory response may be greater with aging.
Pharmacokinetics and Tissue Penetration of Cefoxitin in Obesity (2011)

Obese surgical patients have impaired tissue penetration of the prophylactic antibiotic cefoxitin, and inadequate tissue concentrations despite increased clinical dose (2 g). Inadequate tissue antibiotic concentrations may be a factor in the increased risk of SSIs in obese surgical patients. Additional studies are needed to define doses achieving adequate tissue concentrations.
Hexabenzo[4.4.4]propellane:  A Helical Molecular Platform for the Construction of Electroactive Materials (2007)

Helical hexabenzo[4.4.4]propellane (a relative of hexaphenylethane) and its derivatives are synthesized and their structures are established by X-ray crystallography. Isolation and X-ray crystallographic characterization of a robust trication-radical salt of hexamethoxypropellane derivative confirms that its framework is stable toward oxidative (aliphatic) C-C bond cleavage. It is also demonstrated that propellane can be easily brominated at the 4,4'-positions of the biphenyl linkages for its usage as a molecular platform for the preparation of electroactive materials.
Effective Stabilization of Curcumin by Association to Plasma Proteins: Human Serum Albumin and Fibrinogen (2009)

The use of curcumin as an effective wound healing agent is of significant interest currently. It is well established that curcumin undergoes rapid degradation in physiological buffer by hydrolysis. The means by which curcumin is stabilized at the wound site to enable healing is poorly understood because blood plasma is composed of approximately 92% water. Plasma proteins, which constitute the remaining 6-8%, has been shown to stabilize curcumin. It is, however, still unclear which proteins are responsible for this phenomenon. In this study, the effects of major plasma proteins, which include human serum albumin (HSA), fibrinogen, immunoglobulin G (IgG), and transferrin, on stabilizing curcumin are investigated. In particular, we investigate their effects on the hydrolysis of curcumin at pH 7.4. In the presence of both transferrin and IgG, curcumin continues to undergo rapid hydrolysis but this reaction is suppressed by the presence of either HSA or fibrinogen with an impressive yield of approximately 95%. Furthermore, the binding constants of curcumin to HSA and fibrinogen are on the order of 10(4) and 10(5) M(-1), respectively. The binding constants of transferrin and IgG, however, are at least 1 order of magnitude less than those of HSA and fibrinogen. The results support that strong binding occurs at the hydrophobic moieties of HSA and fibrinogen, excluding water access. Therefore, strong interactions with HSA and fibrinogen inhibit hydrolysis of curcumin and in turn lead to effective suppression of degradation.