gebhart/patrepeval huggingface dataset:
Targeting of metastatic renal cell carcinoma with the chimeric monoclonal antibody G250 labeled with (131)I or (111)In: an intrapatient comparison. (2003)

(111)In-ITC-DTPA-cG250 outperformed (131)I-cG250 for visualization of metastatic RCC lesions, not just because of the superior gamma camera characteristics of (111)In, but more importantly, also because higher tumor:blood ratios were obtained. The higher activities of (111)In-ITC-DTPA-cG250 in metastatic lesions might be caused by internalization and subsequent intracellular retention of the radiolabel, implying that in future radioimmunotherapy trials with cG250 in RCC patients, the use of a residualizing radionuclide should be considered.
Functional characterization of a cAMP-responsive element of the rat insulin I gene. (1990)

Analogues of cAMP have been reported to increase insulin mRNA levels in normal rat beta-cells and hamster insulinoma cells (HIT). To define the mechanisms by which cAMP modulates insulin gene expression, we first investigated its effects on the transcriptional rate of the insulin gene in HIT cells. Nuclear run-on assays revealed a 4-fold increase in transcription observed as early as 1 h after stimulation. To characterize the cis-acting sequences of the rat insulin I gene promoter and the trans-acting factors mediating the cAMP effect on insulin gene transcription, we constructed DNA plasmids containing various lengths of the rat insulin I gene 5'-flanking region linked to the bacterial reporter gene, chloramphenicol acetyltransferase (CAT). Studies of the transcriptional activity of 5'-deletionally and pointly mutated plasmids after transfection into HIT cells revealed the presence of a cAMP-responsive element (CRE), TGACGTCC, between -177 and -184 relative to the transcriptional start site, whose sequence closely matches the previously defined CREs, present in cAMP-responsive genes. Gel retardation and Southwestern assays identify a protein of molecular weight approximately 43,000, binding specifically to the insulin CRE. We conclude that the rat insulin I gene is regulated by cAMP through a CRE and that the nuclear protein interacting with it might be similar or identical to the previously purified cAMP-responsive protein, CREB.
Improving flash write performance by using update frequency (2013)

Solid-state drives (SSDs) are quickly becoming the default storage medium as the cost of NAND flash memory continues to drop. However, flash memory introduces new challenges, as data cannot be eciently updated in-place. To overcome the technology's limitations, SSDs incorporate a software Flash Translation Layer (FTL) that implements out-of-place updates, typically by storing data in a log-structured fashion. Despite a large number of existing FTL algorithms, SSD performance, predictability, and lifetime remain an issue, especially for the write-intensive workloads specific to database applications. In this paper, we show how to design FTLs that are more efficient by using the I/O write skew to guide data placement on flash memory. We model the relationship between data placement and write performance for basic I/O write patterns and detail the most important concepts of writing to flash memory: i) the trade-o between the extra capacity available and write overhead, ii) the benefit of adapting data placement to write skew, iii) the impact of the cleaning policy, and iv) how to estimate the best achievable write performance for a given I/O workload. Based on the findings of the theoretical model, we propose a new principled data placement algorithm that can be incorporated into existing FTLs. We show the benefits of our data placement algorithm when running micro-benchmarks and real database I/O traces: our data placement algorithm reduces write overhead by 20% - 75% when compared to state-of-art techniques.
Building GCC (2004)

Building GCC from scratch is generally perceived as a difficult, complex, and risky undertaking. We respectfully disagree. Admittedly, the process of compiling a compiler from source is complex, but the GNU development team takes care of most of the complexity for you. Building the compiler imposes no risk at all, but installing it, or, rather, installing it incorrectly, can destabilize your system. You can, however, install the newly built compiler as a supplemental or secondary compiler, rather than as the system compiler, and completely sidestep this risk. On the other hand, if you follow the instructions in this chapter, you can install GCC 3.3 as your primary compiler without impacting your system's overall stability. One of the primary points of this chapter is to demonstrate that compiling GCC from scratch is not difficult. It requires some care and attention to detail—and lots of time!—but, as with many tasks, good instructions make it simple to do. If you need some motivation to undertake the upgrade, the section toward the end of this chapter titled "What Is New in GCC 3?" should suffice. It covers a lot of the improvements that make it worthwhile to upgrade.KeywordsSource CodeTest SuiteDisk SpaceSource TreeHome DirectoryThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Magnetic field imaging with microfabricated optically-pumped magnetometers (2017)

A multichannel imaging system is presented, consisting of 25 microfabricated optically-pumped magnetometers. The sensor probes have a footprint of less than 1 cm<sup>2</sup> and a sensitive volume of 1.5 mm × 1.5 mm × 1.5 mm and connect to a control unit through optical fibers of length 5 m. Operating at very low ambient magnetic fields, the sensor array has an average magnetic sensitivity of 24 fT/Hz<sup>1/2</sup>, with a standard deviation of 5 fT/Hz<sup>1/2</sup> when the noise of each sensor is averaged between 10 and 50 Hz. Operating in Earth's magnetic field, the magnetometers have a field sensitivity around 5 pT/Hz<sup>1/2</sup>. The vacuum-packaged sensor heads are optically heated and consume on average 76 ± 7 mW of power each. The heating power is provided by an array of eight diode lasers. Magnetic field imaging of small probe coils was obtained with the sensor array and fits to the expected field pattern agree well with the measured data.
Blockade of T Lymphocyte Costimulation with Cytotoxic T Lymphocyte–Associated Antigen 4–Immunoglobulin (Ctla4ig) Reverses the Cellular Pathology of Psoriatic Plaques, Including the Activation of Keratinocytes, Dendritic Cells, and Endothelial Cells (2000)

Efficient T cell activation is dependent on the intimate contact between antigen-presenting cells (APCs) and T cells. The engagement of the B7 family of molecules on APCs with CD28 and CD152 (cytotoxic T lymphocyte-associated antigen 4 [CTLA-4]) receptors on T cells delivers costimulatory signal(s) important in T cell activation. We investigated the dependence of pathologic cellular activation in psoriatic plaques on B7-mediated T cell costimulation. Patients with psoriasis vulgaris received four intravenous infusions of the soluble chimeric protein CTLA4Ig (BMS-188667) in a 26-wk, phase I, open label dose escalation study. Clinical improvement was associated with reduced cellular activation of lesional T cells, keratinocytes, dendritic cells (DCs), and vascular endothelium. Expression of CD40, CD54, and major histocompatibility complex (MHC) class II HLA-DR antigens by lesional keratinocytes was markedly reduced in serial biopsy specimens. Concurrent reductions in B7-1 (CD80), B7-2 (CD86), CD40, MHC class II, CD83, DC-lysosomal-associated membrane glycoprotein (DC-LAMP), and CD11c expression were detected on lesional DCs, which also decreased in number within lesional biopsies. Skin explant experiments suggested that these alterations in activated or mature DCs were not the result of direct toxicity of CTLA4Ig for DCs. Decreased lesional vascular ectasia and tortuosity were also observed and were accompanied by reduced presence of E-selectin, P-selectin, and CD54 on vascular endothelium. This study highlights the critical and proximal role of T cell activation through the B7-CD28/CD152 costimulatory pathway in maintaining the pathology of psoriasis, including the newly recognized accumulation of mature DCs in the epidermis.
ThruChip Interface for 3D system integration (2010)

ThruChip Interface (TCI) employing inductive coupling bares comparison with Through Silicon Via (TSV) in terms of data rate (11Gb/s/ch), reliability (BER<;10-14), and energy dissipation (0.14pJ/b). It is less expensive than TSV by 20c/chip, though, since it is implemented by digital circuits in a standard CMOS. ESD protection devices can be eliminated to further lower delay, power, and area. It exhibits high noise immunity and alignment tolerance. It provides with an AC coupling link to make interface design easy under multiple/variable VDD's. The cost/performance will further be improved exponentially by thinning chip thickness. Applications include Solid-State Drive (SSD) by stacking NAND Flash memories, a high-speed low-power DRAM interface, bus probing through package for debugging, and non-contact wafer testing. Wireless power delivery by using inductive coupling will further widen the applications. This talk will cover basics, applications, and future perspectives of the TCI.
Synthesis of Phosphatidylinositol 3-Kinase (PI3K) Inhibitory Analogues of the Sponge Meroterpenoid Liphagal (2010)

Analogues of the sponge meroterpenoid liphagal have been synthesized and evaluated for inhibition of PI3Kα and PI3Kγ as part of a program aimed at developing new isoform-selective PI3K inhibitors. One of the analogues, compound 24, with IC₅₀ values of 66 nM against PI3Kα and 1840 nM against PI3Kγ, representing a 27-fold preference for PI3Kα, exhibited enhanced chemical stability and modestly enhanced potency and selectivity compared with the natural product liphagal.
Blocking translocation of cell surface molecules from the ER to the cell surface by intracellular antibodies targeted to the ER (2007)

Intracellular antibodies (intrabodies) constitute a potent tool to neutralize the function of target proteins inside specific cell compartments (cytosol, nucleus, mitochondria and ER). The intrabody technology is an attractive alternative to the generation of gene-targeted knockout animals and complements or replaces knockdown techniques such as antisense-RNA, RNAi and RNA aptamers. This article focuses on intrabodies targeted to the ER. Intracellular anti-bodies expressed and retained inside the ER (ER intrabodies) are shown to be highly efficient in blocking the translocation of secreted and cell surface molecules from the ER to the cell surface. The advantage of ER intrabodies over cytoplasmic intrabodies is that they are correctly folded and easier to select. A particular advantage of the intrabody technology over existing ones is the possibility of inhibiting selectively post-translational modifications of proteins. The main applications of ER intrabodies so far have been (i) inactivation of oncogenic receptors and (ii) functional inhibition of virus envelope proteins and virus-receptor molecules on the surface of host cells. In cancer research, the number of in vivo mouse models for evaluation of the therapeutic potential of intrabodies is increasing. In the future, endosomal localized receptors involved in bacterial and viral infections, intracellular oncogenic receptors and enzymes involved in glycosylation of tumour antigens might be new targets for ER intrabodies.
Potential Anticancer Agents.1 LXXVII. Synthesis of Nucleosides of Purine-6-thiol (6-Mercaptopurine) Containing “Fraudulent” Sugars (1962)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPotential Anticancer Agents.1 LXXVII. Synthesis of Nucleosides of Purine-6-thiol (6-Mercaptopurine) Containing "Fraudulent" SugarsElmer J. Reist, Allen Benitez, William W. Lee, B. R. Baker, and Leon GoodmanCite this: J. Org. Chem. 1962, 27, 9, 3279–3283Publication Date (Print):September 1, 1962Publication History Published online1 May 2002Published inissue 1 September 1962https://pubs.acs.org/doi/10.1021/jo01056a072https://doi.org/10.1021/jo01056a072research-articleACS PublicationsRequest reuse permissionsArticle Views79Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A comprehensive nomenclature for serine proteases with homology to tissue kallikreins (2006)

The human kallikrein locus on chromosome 19q13.3-13.4 contains kallikrein 1--the tissue kallikrein--and 14 related serine proteases. Recent investigations into their function and evolution have indicated that the present nomenclature for these proteins is inadequate or insufficient. Here we present a new nomenclature in which proteins without proven kininogenase activity are denoted kallikrein-related peptidase. Names are also given to the unique rodent proteins that are closely related to kallikrein 1.
Structural effects induced by removal of a disulfide-bridge: the X-ray structure of the C30A/C51A mutant of basic pancreatic trypsin inhibitor at 1.6 Å (1990)

The X-ray structure of a variant of basic pancreatic trypsin inhibitor (BPTI) has been analyzed to determine the structural accommodation resulting from removal of a disulfide cross-link in a protein. The disulfide removed, Cys30-Cys51, has been implicated in both the folding pathway of the protein and its overall thermal stability. In the variant studied, C30A/C51A, the disulfide cysteines were replaced by less bulky alanines. The atomic displacements observed for C30A/C51A indicate a set of concerted shifts of two segments of chains, which together significantly diminish a packing defect at the site of the removed cysteine sulfur atoms. The observed structural changes are distributed asymmetrically around the sites of mutation, indicating that the adjacent beta-sheet is more resistant to the perturbation than the alpha-helix on the opposite side of the disulfide bond. The thermal parameters of groups involved in the structural accommodation are not significantly altered. A comparison of the X-ray structures reported for native BPTI determined in three different crystal forms indicates that the magnitude of its conformational variability exceeds that of the structural changes caused by the disulfide removal. This emphasizes the necessity of using isomorphous crystal systems to determine the relatively small effects due to mutation.
Eleven Plasma Proteins as Indicators of Protein Nutritional Status in Very Low Birth Weight Infants (1990)

Concentrations of 11 plasma proteins were measured in 28 healthy, growing, very low birth weight, appropriate-for-gestational-age infants fed varying levels of human milk protein intake (range 1.7 to 3.9 g/kg per day). Significant positive correlations were found between mean protein intake and concentrations of 7 of the plasma proteins studied (transthyretin, retinol-binding protein, and transferrin: P less than .001; vitamin D-binding protein and apolipoprotein B: P less than .01; albumin and apolipoprotein A I: P less than .05). A weak negative correlation with mean protein intake was seen for the plasma level of orosomucoid, whereas no significant correlations were found for the plasma concentrations of fibronectin and alpha 1-antichymotrypsin. Protein intake, not energy intake, constituted the main contribution to the changes in the concentrations of transthyretin, retinol-binding protein, and transferrin. The levels of plasma transthyretin and transferrin were also strongly correlated with weight and length growth of the infants during the study as well as with other indicators of protein nutritional status such as preprandial concentrations of plasma amino acids and serum and urine urea. These data indicate that of the 11 plasma proteins studied, transthyretin, transferrin, and retinol-binding protein are the most suitable to evaluate protein nutritional status in very low birth weight infants.
The role of HLA class I antigens in recognition of melanoma cells by tumor-specific cytotoxic T lymphocytes. Evidence for shared tumor antigens. (1989)

CTL lines were established in vitro by stimulating patient lymphocytes with autologous melanoma cells in the presence of IL-2. Resulting CTL lines lysed autologous melanoma and failed to lyse several allogeneic melanomas or K562. The mechanism of target cell recognition by autologous tumor-specific CTL was evaluated in this system, using several CTL lines: DT6, DT105, DT141, DT166, DT169, and DT179. Autologous melanoma lysis was inhibited by W6/32, mAb directed against HLA class I Ag, but not by L243, mAb directed against HLA class II Ag. CTL from DT6, DT141, DT166, DT169, and DT179 lysed fresh and cultured allogeneic melanomas, which shared the HLA-A2 Ag, but failed to lyse allogeneic melanomas, which shared B-region or C-region Ag, or shared no HLA class I Ag. CTL from DM141 lysed DM93, which shared A2 and Bw6, but failed to lyse DM105, which shared only Bw6. DM105 CTL failed to lyse allogeneic melanomas that shared HLA-A1, or that shared B or C region Ag, but they did lyse allogeneic melanoma DM49, which expressed an A region Ag that either was A10 or was serologically cross-reactive with A10. A T cell leukemia line, three EBV transformed B cell lines, and a pancreatic cancer line, all of which expressed HLA-A2, were not lysed by DM6 or DM179 CTL. Furthermore, HLA-matched nonmelanomas failed to inhibit autologous tumor lysis in cold target inhibition assays, whereas an HLA-A2+ allogeneic melanoma, DM93, inhibited autologous tumor lysis as effectively as the autologous tumor itself. HLA-A2, and possibly other HLA-A-region Ag, appear to function in HLA-restricted recognition of shared melanoma associated Ag by CTL.
A dichotomous role for nitric oxide during acuteToxoplasma gondiiinfection in mice (1997)

Production of nitric oxide by macrophages is believed to be an important microbicidal mechanism for a variety of intracellular pathogens, including Toxoplasma gondii. Mice with a targeted disruption of the inducible nitric oxide synthase gene (iNOS) were infected orally with T. gondii tissue cysts. Time to death was prolonged compared with parental controls. Histologic analysis of tissue from infected mice showed scattered small foci of inflammation with parasites in various tissues of iNOS-/- mice, whereas tissue from the parental C57BL/6 mice had more extensive tissue inflammation with few visible parasites. In particular, extensive ulceration and necrosis of distal small intestine and fatty degeneration of the liver was seen in the parental mice at day 7 postinfection, as compared with the iNOS-/- mice where these tissues appeared normal. Serum interferon gamma and tumor necrosis factor alpha levels postinfection were equally elevated in both mouse strains. Treatment of the parental mice with a NO synthase inhibitor, aminoguanidine, prevented early death in these mice as well as the hepatic degeneration and small bowel necrosis seen in acutely infected control parentals. These findings indicate that NO production during acute infection with T. gondii can kill intracellular parasites but can be detrimental, even lethal, to the host.
Postgrowth tuning of cavity resonance for multiple-wavelength laser and detector arrays (1998)

Summary form only given. Multiple-wavelength arrays of vertical-cavity lasers (VCLs) and detectors are important for wavelength-division-multiplexing systems. In-plane variation of the cavity resonant wavelength usually requires growth on patterned substrates or two successive growths and a complicated processing. It was recently observed that the lateral wet oxidation of an AlGaAs layer can result in partial vertical oxidation of an adjacent layer. We propose to use this combined lateral-vertical oxidation to change the optical thickness of a cavity after the epitaxial growth of both Bragg mirrors.
P3HT:PCBM, Best Seller in Polymer Photovoltaic Research (2011)

In the fi eld of polymer-based photovoltaic cells, poly(3-hexylthiophene) (P3HT) and 1-(3-methoxycarbonyl)propyl-1-phenyl[6,6]C₆₁ (PCBM) are, to date, the most-studied active materials around the world for the bulk-heterojunction structure. Various power-conversion effi ciencies are reported up to approximately 5%. This Research News article is focused on a survey of the tremendous literature published between 2002 and 2010 that exhibits solar cells based on blends of P3HT and PCBM.
An automated front‐end monitor for anthrax surveillance systems based on the rapid detection of airborne endospores (2007)

A fully automated anthrax smoke detector (ASD) has been developed and tested. The ASD is intended to serve as a cost effective front-end monitor for anthrax surveillance systems. The principle of operation is based on measuring airborne endospore concentrations, where a sharp concentration increase signals an anthrax attack. The ASD features an air sampler, a thermal lysis unit, a syringe pump, a time-gated spectrometer, and endospore detection chemistry comprised of dipicolinic acid (DPA)-triggered terbium ion (Tb(3+)) luminescence. Anthrax attacks were simulated using aerosolized Bacillus atrophaeus spores in fumed silica, and corresponding Tb-DPA intensities were monitored as a function of time and correlated to the number of airborne endospores collected. A concentration dependence of 10(2)-10(6) spores/mg of fumed silica yielded a dynamic range of 4 orders of magnitude and a limit of detection of 16 spores/L when 250 L of air were sampled. Simulated attacks were detected in less than 15 min.