gebhart/patrepeval huggingface dataset:
Novel Synthesis of Poly(p-phenylene sulfide) from Cyclic Disulfide Oligomers (1996)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTNovel Synthesis of Poly(p-phenylene sulfide) from Cyclic Disulfide OligomersYong Ding and Allan S. HayView Author Information Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, PQ H3A 2K6, Canada Cite this: Macromolecules 1996, 29, 13, 4811–4812Publication Date (Web):June 17, 1996Publication History Received7 February 1996Revised17 April 1996Published online17 June 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/ma960195ohttps://doi.org/10.1021/ma960195orapid-communicationACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views934Altmetric-Citations40LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Aromatic compounds,Disulfides,Oligomers,Polymers,Sulfides Get e-Alerts
Establishment of mouse induced pluripotent stem cells selected for Nanog expression (2008)

Embryonic stem (ES)-like cells can be induced from either mouse embryonic or adult fibroblasts by introducing four factors (Oct3/4, Sox2, c-Myc, and Klf4) and by selection for Fbx15 expression. These Fbx15 PS (induced pluripotent stem) cells are similar to ES cells in their morphology, proliferation and teratoma formation. Fbx15 PS cells, however, showed a different gene expression pattern from ES cells and they failed to produce adult chimeras. More ES-like PS cells might be generated by using a more ES specific marker than Fbx15. Based on this hypothesis, we focused on Nanog in this study, because of its essential role in pluripotency. Embryonic fibroblasts (MEF) were isolated from Nanog reporter mice, and Nanog PS cells were established by retrovirus-mediated transfection of the aforementioned four factors. Nanog PS cells showed morphology, proliferation, and teratoma formation similar to those of ES cells. These Nanog PS cells highly expressed ES cell markers, such as Nanog, Fbx15, ERas, and ESG1. Transgene expression of the four factors was strongly silenced in Nanog PS cells. When transplanted into blastocysts, adult chimeric mice were obtained from Nanog PS cells. Moreover, Nanog PS cells were transmitted through the germline to the next generation from the chimeric mice. Around 20% of the offspring, however, formed tumors, in which the reactivation of retroviral c-Myc was observed. These data demonstrated that germ-line competent PS cells can be obtained from MEF by the four factors and Nanog-selection. However, the retroviral transduction of c-Myc should be replaced by other methods prior to future medical application.
Functional and Transcriptional Characterization of Human Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Myocardial Infarction (2009)

Taken together, we report a more efficient method for derivation of hESC-ECs that express appropriate patterns of endothelial genes, form functional vessels in vivo, and improve cardiac function. These studies suggest that hESC-ECs may provide a novel therapy for ischemic heart disease in the future.
Wide field of view catadioptrical Head Mounted Display (2002)

人工現実感や複合現実感, ロボットのテレオペレーションなどの応用において, ヘッドマウントディスブレイ (HMD) が, 数多く利用されている。しかし, その多くは, 視野角が60度程度と狭く, 人に与える臨場感が低いという問題を持っていた。本研究では, 曲面ミラーを組み合わせることで約180度の水平視野を実現した広視野角HMDを提案する。
Short low- and medium-frequency antenna performance (2005)

Lately, short antennas have attracted the attention of the broadcast and communication communities. This kind of antenna has been used since the 1920s. Top-loaded monopoles are the logical antennas to be used in order to get a low-profile antenna and performance according to the needs of the broadcaster and for communication. In this paper, top-loaded monopoles have been studied exhaustively using the transmission-line technique. Improved expressions for the antenna's radiation resistance have been obtained, taking into account the top-base current relationship and under different top-loading conditions. This idea, of using an equivalent transmission-line technique, has been used since the 1920s in order to obtain the antenna's input reactance. Using this old idea, the novel approach here permits obtaining the near- and far-field expressions from the current distribution on the antenna structure. A near-field calculation is used to determine the surface-current density on the ground plane. The power dissipation is calculated from the artificial and natural ground-plane surface-current densities, and the ground plane equivalent loss resistance is obtained. In all cases, as a first approximation, a half-wavelength ground-plane radius has been used, because this is the maximum distance covered by the ground surface current under the antenna, closing the antenna's electric circuit. Beyond this distance, the ground currents do not return to the antenna generator, and are taken into account in the surface-wave propagation calculations. The half-wavelength ground-plane surface is partially occupied by the metallic radial ground system, and the remainder consists of the natural soil. Artificial ground-plane behavior is paramount in obtaining the best performance for a short antenna. This kind of antenna could perform very close to a standard quarter-wave monopole, if it has optimum dimensions. For these reasons, a short antenna and the corresponding artificial ground plane have been analyzed, modifying the number of radials and their lengths, in order to achieve optimum performance, or to obtain maximum field strength with several soil conditions for the Earth's surface. A very simple and efficient antenna can be obtained, giving to the broadcast and communication communities a product that can fulfil the required performance of radiating a high-quality AM signal or digital transmission in the MF band, and good speech quality in the LF band.
mRNA Capping Enzyme Requirement forCaenorhabditis elegans Viability (2003)

Capping of the initiated 5' ends of RNA polymerase II products is evolutionarily and functionally conserved from yeasts to humans. The m(7)GpppN cap promotes RNA stability, processing, transport, and translation. Deletion of capping enzymes in yeasts was shown to be lethal due to rapid exonucleolytic degradation of uncapped transcripts or failure of capped but unmethylated RNA to initiate protein synthesis. Using RNA interference and Caenorhabditis elegans we have found that RNA capping is also essential for metazoan viability. C. elegans bifunctional capping enzyme was cloned, and capping activity by the expressed protein as well as growth complementation of yeast deletion strains missing either RNA triphosphatase or guanylyltransferase required terminal sequences not present in the previously isolated cel-1 clone. By RNA interference analysis we show that cel-1 is required for embryogenesis. cel-1(RNAi) embryos formed cytoplasmic granules characteristic of a phenocluster of RNA processing genes and died early in development.
Stomach Organ and Cell Lineage Differentiation: From Embryogenesis to Adult Homeostasis (2016)

Gastric diseases cause considerable worldwide burden. However, the stomach is still poorly understood in terms of the molecular-cellular processes that govern its development and homeostasis. In particular, the complex relationship between the differentiated cell types located within the stomach and the stem and progenitor cells that give rise to them is significantly understudied relative to other organs. In this review, we will highlight the current state of the literature relating to specification of gastric cell lineages from embryogenesis to adulthood. Special emphasis is placed on substantial gaps in knowledge about stomach specification that we think should be tackled to advance the field. For example, it has long been assumed that adult gastric units have a granule-free stem cell that gives rise to all differentiated lineages. Here we will point out that there are also other models that fit all extant data, such as long-lived lineage-committed progenitors that might serve as a source of new cells during homeostasis.
A Simple Method for Delivering Morpholino Antisense Oligos into the Cytoplasm of Cells (1996)

We report a simple and effective means for delivering Morpholino antisense oligos into the cytosol of cultured anchorage-dependent animal cells. This method, referred to as scrape-loading, is carried out in a matter of seconds, uses a common inexpensive laboratory implement, and has minimal detrimental impact on the cells. Using this delivery method, a Morpholino oligo present at 0.1 microM and 1 microM in the extracellular medium inhibited its targeted genetic sequence within cultured Hela cells at levels of 56% and 85%, respectively. Lack of inhibition by two control Morpholino oligos at concentrations up to 3 microM indicates good sequence specificity by this structural type. Also described is a test system for simple, rapid, and sensitive quantitation of antisense activity in cultured cells.
Single-shot measurement of temporal phase shifts by frequency-domain holography (2000)

Frequency-domain holography is used to measure ultrafast phase shifts induced either by the nonlinear susceptibility ?(3) of fused silica or by ionization fronts in air over a temporal region of 1 ps with 70-fs resolution in a single shot. The use of an imaging spectrometer adds one-dimensional spatial resolution to the single-shot temporal measurements.
Photoinduced Magnetization in Copper Octacyanomolybdate (2005)

This article describes the studies of a photomagnetic cyanide-bridged Cu-Mo bimetallic assembly, Cu(II)(2)[Mo(IV)(CN)(8)].8H(2)O (Cu(II), S = (1)/(2); Mo(IV), S = 0) (1), which has an intervalence transfer (IT) band from Mo(IV)-CN-Cu(II) to Mo(V)-CN-Cu(I) around 480 nm. Wide-angle X-ray scattering and X-ray spectroscopic studies provide precise information about the 3D connectivity and the local environment of the transition metal ions. Irradiating with blue light causes solid 1 to exhibit a spontaneous magnetization (Curie temperature = 25 K). The thermal reversibility is carefully studied and shows the long-time stability of the photoinduced state up to 100 K. Photoreversibility is also observed; i.e., the magnetization is induced by irradiation with light below 520 nm, while the magnetization is reduced by irradiation with light above 520 nm. The UV-vis absorption spectrum after irradiation shows a decrease of the IT band and the appearance of the reverse-IT band in the region of 600-900 nm (lambda(max) = 710 nm). This UV-vis absorption spectrum is recovered to the original spectrum by irradiation with 658-, 785-, and 840-nm light. In this photomagnetic effect, the excitation of the IT band causes an electron transfer from Mo(IV) to Cu(II), producing a ferromagnetic mixed-valence isomer of Cu(I)Cu(II)[Mo(V)(CN)(8)].8H(2)O (Cu(I), S = 0; Cu(II), S = (1)/(2); Mo(V), S = (1)/(2)) (1'). 1' returns to 1 by irradiation of the reverse-IT band, which obeys the scheme for the potential energy surface in mixed-valence class II compounds.
Notch signaling modulates proliferation and differentiation of intestinal crypt base columnar stem cells (2011)

Notch signaling is known to regulate the proliferation and differentiation of intestinal stem and progenitor cells; however, direct cellular targets and specific functions of Notch signals had not been identified. We show here in mice that Notch directly targets the crypt base columnar (CBC) cell to maintain stem cell activity. Notch inhibition induced rapid CBC cell loss, with reduced proliferation, apoptotic cell death and reduced efficiency of organoid initiation. Furthermore, expression of the CBC stem cell-specific marker Olfm4 was directly dependent on Notch signaling, with transcription activated through RBP-Jκ binding sites in the promoter. Notch inhibition also led to precocious differentiation of epithelial progenitors into secretory cell types, including large numbers of cells that expressed both Paneth and goblet cell markers. Analysis of Notch function in Atoh1-deficient intestine demonstrated that the cellular changes were dependent on Atoh1, whereas Notch regulation of Olfm4 gene expression was Atoh1 independent. Our findings suggest that Notch targets distinct progenitor cell populations to maintain adult intestinal stem cells and to regulate cell fate choice to control epithelial cell homeostasis.
Structure Change Associated with the [MII/III 1,4,7-Triazacyclononane-N,N′,N′′-triacetate (TCTA)]−/0 Electron Transfers (M = Mn, Fe, and Ni): Crystal Structure for [FeII(H2O)6][FeII(TCTA)]2 (2010)

Heterogeneous electron-transfer rate measurements using the scanning electrochemical microscope are reported for the [M(TCTA)](-/0) couples (M = Mn, Fe, and Ni) in aqueous solution. Solution IR spectroscopy indicates that N(3)O(3) coordination is preserved for each couple within the pH range of 2-4, and susceptibility measurements indicate little or no interference from spin-state changes at room temperature. Marcus-Hush expressions were used to quantitatively relate structural differences between oxidation states to measured standard heterogeneous electron-transfer rate constants. Good correlation was obtained for the Fe couple, and structural changes associated with the Mn and Ni couples were estimated. In addition, the structure of the Fe(II) complex was determined by X-ray crystallography. The molecule [Fe(H(2)O)(6)][Fe(TCTA)](2) is trigonal, space group P3(1)/c (no. 159) with a = b = 12.530(3) Å, c = 12.656(4) Å, and Z = 2. A notable feature of the structure is that the [Fe(TCTA)](-) complex is distributed between two different geometries, one being rigorously trigonal prismatic and the other having a 26° antiprismatic twist.
Methods for Measurement of Intracellular Magnesium:NMR and Fluorescence (1991)

Macrophage polarization refers to how macrophages have been activated at a given point in space and time. Polarization is not fixed, as macrophages are sufficiently plastic to integrate multiple signals, such as those from microbes, damaged tissues, and ...Read More
Depicting Safety Profile of TAAR1 Agonist Ulotaront Relative to Reactions Anticipated for a Dopamine D2-Based Pharmacological Class in FAERS (2021)

NCT0296938, NCT00088634, NCT00549718, NCT00615433, NCT00790192.
Calix[3]indoles, new macrocyclic tris(indolylmethylene) compounds with 2,7-linkages (1993)

A series of macrocyclic tris(indolylmethylene) compounds (calix[3]indoles] can be obtained from 7- or 2-hydroxymethylindoles or from the combination of either an indole with a bis(hydroxymethyl)-2,7′-diindolylmethane or a bis(hydroxymethyl)indole with a 2,7′-diindolylmethane; an isomeric series can be obtained from the combination of an indole with a bis(hydroxymethyl)-2,2′-diindolylmethane.
TCR β-Chain Sharing in Human CD8+ T Cell Responses to Cytomegalovirus and EBV (2008)

The CD8(+) TCR repertoires specific for many immunogenic epitopes of CMV and EBV are dominated by a few TCR clonotypes and involve public TCRs that are shared between many MHC-matched individuals. In previous studies, we demonstrated that the observed sharing of epitope-specific TCRbeta chains between individuals is strongly associated with TCRbeta production frequency, and that a process of convergent recombination facilitates the more efficient production of some TCRbeta sequences. In this study, we analyzed a total of 2836 TCRbeta sequences from 23 CMV-infected and 10 EBV-infected individuals to investigate the factors that influence the sharing of TCRbeta sequences in the CD8(+) T cell responses to two immunodominant HLA-A*0201-restricted epitopes from these viruses. The most shared TCRbeta amino acid sequences were found to have two features that indicate efficient TCRbeta production, as follows: 1) they required fewer nucleotide additions, and 2) they were encoded by a greater variety of nucleotide sequences. We used simulations of random V(D)J recombination to demonstrate that the in silico TCRbeta production frequency was predictive of the extent to which both TCRbeta nucleotide and amino acid sequences were shared in vivo. These results suggest that TCRbeta production frequency plays an important role in the interindividual sharing of TCRbeta sequences within CD8(+) T cell responses specific for CMV and EBV.
Hypothermia for the treatment of ischemic and hemorrhagic stroke (2009)

Hypothermia is considered nature's "gold standard" for neuroprotection, and its efficacy for improving outcome in patients with hypoxic-ischemic brain injury as a result of cardiac arrest is well-established. Hypothermia reduces brain edema and intracranial pressure in patients with traumatic brain injury. By contrast, only a few small pilot studies have evaluated hypothermia as a treatment for acute ischemic stroke, and no controlled trials of hypothermia for hemorrhagic stroke have been performed. Logistic challenges present an important barrier to the widespread application of hypothermia for stroke, most importantly the need for high-quality critical care to start immediately in the emergency department. Rapid induction of hypothermia within 3 to 6 hrs of onset has been hampered by slow cooling rates, but is feasible. Delayed cooling for the treatment of cytotoxic brain edema does not provide definitive or lasting treatment for intracranial mass effect, and should not be used as an alternative to hemicraniectomy. Sustained fever control is feasible in patients with intracerebral and subarachnoid hemorrhage, but has yet to be tested in a phase III study. Important observations from studies investigating the use of hypothermia for stroke to date include the necessity for proactive antishivering therapy for successful cooling, the importance of slow controlled rewarming to avoid rebound brain edema, and the high risk for infectious and cardiovascular complications in this patient population. More research is clearly needed to bring us closer to the successful application of hypothermia in the treatment for stroke.
Trk inhibitor attenuates the BDNF/TrkB-induced protection of neuroblastoma cells from etoposidein vitroandin vivo (2015)

TrkB activation by brain-derived neurotrophic factor (BDNF) contributes to chemo-resistance in neuroblastoma (NB). AZD6918 is a novel potent and selective inhibitor of the Trk tyrosine kinases. In this study we evaluated the effect of AZD6918 on the sensitivity of TrkB-expressing NB cells or tumors to etoposide, a topoisomerase II inhibitor. TrkB-expressing NB cells were treated with AZD6918 and etoposide in the presence or absence of BDNF in vitro and cell survival was determined. NB xenograft tumors were treated with AZD6918 and etoposide, either alone or in combination in vivo, and the anti-tumor growth effect or mice survival advantage was evaluated. Our study showed that AZD6918 induced cell death as a single agent and attenuated BDNF/TrkB-induced protection from etoposide in vitro. Although AZD6918 alone didn't show anti-tumor growth effect or survival advantage in vivo, a combination of AZD6918 and etoposide had a statistically significant stronger anti-tumor growth effect and survival advantage compared to treatment with either agent alone. Our data indicate that as a Trk inhibitor AZD6918 increased the sensitivity of NB to etoposide. These results extend the spectrum of cytotoxic drugs whose efficacy is increased in combination with Trk inhibitors and support the combination of Trk inhibitors and cytotoxic drugs for NB treatment.
Networked MIMO with clustered linear precoding (2009)

A clustered base transceiver station (BTS) coordination strategy is proposed for a large cellular MIMO network, which includes full intra-cluster coordination-to enhance the sum rate-and limited inter-cluster coordination-to reduce interference for the cluster edge users. Multi-cell block diagonalization is used to coordinate the transmissions across multiple BTSs in the same cluster. To satisfy per-BTS power constraints, three combined precoder and power allocation algorithms are proposed with different performance and complexity tradeoffs. For inter-cluster coordination, the coordination area is chosen to balance fairness for edge users and the achievable sum rate. It is shown that a small cluster size (about 7 cells) is sufficient to obtain most of the sum rate benefits from clustered coordination while greatly relieving channel feedback requirement. Simulations show that the proposed coordination strategy efficiently reduces interference and provides a considerable sum rate gain for cellular MIMO networks.
Recombinant human leukocyte interferon produced in bacteria has antiproliferative activity. (1981)

Recombinant human leukocyte interferon synthesized by Escherichia coli possesses antiproliferative activity in addition to antiviral activity. When the ability to inhibit multiplication of lymphoblastoid Daudi cells was examined, the growth-inhibitory capacity of recombinant leukocyte interferon was equivalent to that exhibited by crude human leukocyte interferon or by the homogeneous gamma 2 species of leukocyte interferon synthesized by human cells.
Purification and characterization of active and latent forms of recombinant plasminogen activator inhibitor 1 produced in Escherichia coli (1992)

Plasminogen activator inhibitor 1 (PAI-1), the principal physiological inhibitor of tissue plasminogen activator (tPA), is a protein of 379 amino acids and belongs to the SERPIN family of serine protease inhibitors. We have previously described methods to express [Sisk et al. (1990) Gene 96, 305-309] and purify [Reilly et al. (1990) J. Biol. Chem. 265, 9570-9574] a highly active form of the protein in substantial amounts, from Escherichia coli. Further analyses of this material showed the presence of small but significant amounts of latent rPAI-1. The present paper describes for the first time purification of latent and active forms of rPAI-1 from a single preparation, as well as the functional and structural characteristics of the two forms. Latent rPAI-1, which has properties similar to the latent forms described by other groups, was separated from active rPAI-1 by high-resolution ion-exchange chromatography or by affinity chromatography using immobilized anhydrotrypsin. It had low intrinsic activity (< 5% of active rPAI-1) and was partially reactivated by guanidine hydrochloride treatment or by incubation with vitronectin. Conversion of the active rPAI-1 to the latent form was influenced by temperature and additives including sucrose, EDTA, and arginine. Active and latent rPAI-1 did not show any obvious differences in their primary structures and displayed remarkably similar secondary structures as determined by circular dichroism spectral analyses. However, they did exhibit differences in tryptophan fluorescence, suggesting tertiary structural differences between the two forms.