gebhart/patrepeval huggingface dataset:
Investigation into possible causes of interference in serum testosterone measurement in women (2006)

Given that DHEA-S is present in very variable amounts in blood, it is possible that even with a low cross reactivity, DHEA-S or one of its metabolites significantly interferes in the testosterone assay when at higher concentrations.
Roles of histamine, complement and xanthine oxidase in thermal injury of skin. (1989)

The pathogenesis of burn edema in the skin of rats appears to be related to a role for histamine, xanthine oxidase and oxygen radicals. Histamine and its metabolic derivatives increase the catalytic activity of xanthine oxidase (but not xanthine dehydrogenase) in rat plasma and in rat pulmonary artery endothelial cells. In thermally injured rats levels of plasma histamine and xanthine oxidase rise in parallel, in association with increases in uric acid. Burn edema is greatly attenuated by treatment of rats with the mast cell stabilizer, cromolyn, by complement depletion and by treatment with the H2 receptor antagonist, cimetidine, but is unaffected by neutrophil depletion. These studies suggest the following pathogenesis of burn edema: thermal trauma causes complement activation with anaphylatoxin release and mast cell secretion of histamine, leading to enhancement of xanthine oxidase activity and increased production of oxygen radicals which damage endothelial cells.
Formation of Titanium Oxide Nanotube (1998)

Nanotubes composed of various materials such as carbon, boron nitride, and oxides have been studied recently. In this report, the discovery of a new route for the synthesis of a nanotube made of titanium oxide is presented. Needle-shaped TiO2 crystals (anatase phase) with a diameter of ≈8 nm and a length of ≈100 nm were obtained when sol−gel-derived fine TiO2-based powders were treated chemically (e.g., for 20 h at 110 °C) with a 5−10 M NaOH aqueous solution. It was found by observation using a transmission electron microscope that the needle-shaped products have a tube structure. The TiO2 nanotubes have a large specific surface area of ≈400 m2·g-1. TiO2 nanotubes obtained in the present work are anticipated to have great potential for use in the preparation of catalysts, adsorbants, and deodorants with high activities, because their specific surface area is greatly increased. If metallic-, inorganic-, or organic-based materials can be inserted into the TiO2 nanotubes, novel characteristics such as electric, electromagnetic, or chemical properties may be induced in the TiO2 materials.
Paper Spray Ionization of Noncovalent Protein Complexes (2014)

Paper spray (PS) ionization, an ambient ionization method, has previously been explored as a direct and fast method for mass spectrometric analysis of complex mixtures. It has been applied to the analysis of a wide variety of compounds, mostly small molecules. The work reported here extends the application of PS ionization to noncovalent protein complexes on an ion mobility tandem mass spectrometer. Similar mass spectra for protein complexes were obtained by PS ionization and nanoflow electrospray ionization (nESI), indicating that intact protein complexes can be preserved in PS ionization. In addition, collisional cross sections measured by ion mobility provide evidence that the protein assemblies may remain compact by PS ionization. With PS, it is possible to detect hemoglobin tetramer from a blood sample with minimal sample preparation. This is the first report to show that PS ionization is a promising ionization method for nonconvalent protein complexes.
Ketone-Catalyzed Asymmetric Epoxidation Reactions (2004)

This Account summarizes our efforts in developing organic ketone catalysts for enantioselective and diastereoselective epoxidation of olefins. We have developed an efficient and general protocol for epoxidation of olefins using dioxiranes generated in situ from activated ketones and Oxone (2KHSO5.KHSO4.K2SO4) in a homogeneous aqueous solvent system. We have also developed chiral ketone catalysts for highly enantioselective epoxidation of unfunctionalized trans-olefins and trisubstituted olefins. Excellent diastereoselectivities have been achieved for epoxidation of substituted cyclohexenes with ketone catalysts.
High-speed analog complex-amplitude liquid-crystal light modulator (1994)

A homogeneously aligned smectic A* liquid-crystal quarter-wave retarder is used to produce analog complex-amplitude modulation of light through the electroclinic effect. The device consists of a reflection-mode polarization rotative phase shifter enhanced by an asymmetric resonator. With an entrance mirror reflectivity of 70%, an analog modulation of 65% of a fringe was observed. The 10-90% response time of the liquid crystal was measured to be 25 micros at 25 degrees C.
New Aspects of Combining Echo Cancellers with Beamformers (2006)

In this contribution, we introduce a novel framework for combining approaches for acoustic echo cancellation and beamforming. Classical schemes of combination incorporate the simple concatenation of both subsystems. However, if the echo cancellers come first, they cannot exploit the noise reduction capabilities of the beamformer. In the other setup, a time-variant beamformer can heavily disturb the succeeding echo canceller's convergence. The new approach establishes a possibility to smoothly switch between two possible target functions: the mean squared errors at the beamformer's inputs and its output, respectively. Thus, one combined system benefits from advantages of both classical schemes. The paper contains a theoretical analysis of possible solutions for preceding echo cancellers, which are adapted using the beamformer output. The appropriate modification of the normalized least mean squares algorithm is derived too.
Refining the Prostate Cancer Genetic Association within the JAZF1 Gene on Chromosome 7p15.2 (2010)

Future studies should identify all variants in high linkage disequilibrium with rs10486567 and evaluate their functional significance for PrCa.
Aggregation of nickel(II), copper(II), and zinc(II) derivatives of water-soluble porphyrins (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAggregation of nickel(II), copper(II), and zinc(II) derivatives of water-soluble porphyrinsRobert F. Pasternack, Lynn Francesconi, Don Raff, and Esther SpiroCite this: Inorg. Chem. 1973, 12, 11, 2606–2611Publication Date (Print):November 1, 1973Publication History Published online1 May 2002Published inissue 1 November 1973https://doi.org/10.1021/ic50129a023Request reuse permissionsArticle Views1594Altmetric-Citations192LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (719 KB) Get e-Alertsclose Get e-Alerts
A study of biofilm-based wound management in subjects with critical limb ischaemia (2008)

Objective: Bacterial biofilms cause or complicate numerous medical conditions, including chronic wounds. Biofilm-based wound care (BBWC) management strategies that suppress biofilm have been designed and are used extensively at the Southwest Regional Wound Care Center in Lubbock, Texas and are described in this article. This retrospective single-centre study was designed to evaluate the frequency of complete healing in subjects with a chronic wound in a limb with critical limb ischaemia (CLI) when managed using BBWC. Method: Of the 4500 subjects admitted with wounds between August 2002 and January 2006, 1400 subjects’ TCpO2 levels were measured, and 266 included were identified as having CLI (TCpO2 &lt;20mmHg). Of these, 190 subjects were considered in the analysis because they received a substantial course of therapy (more than five visits). Each subject was individually managed to reinforce natural healing and suppress bacterial biofilm. Successful healing was defined as complete closure by March 2007. Results: Of the 190 subjects with CLI, 146 (77%) healed completely, and 44 (23%) were categorised as non-healing. The healed group included 47% (68/146) with osteomyelitis and 69% (101/146) with diabetes mellitus. In the non-healed group, 75% (33/44) had osteomyelitis and 77% (34/44) had diabetes mellitus. Ninety-one per cent (30/33) of the subjects without osteomyelitis or diabetes mellitus healed, and 67% (53/79) of the subjects with both osteomyelitis and diabetes mellitus healed. Conclusion: When comparing the healing frequency in this study with a previously published study, BBWC strategies significantly improved healing frequency. These findings demonstrate that effectively managing the biofilm in chronic wounds is an important component of consistently transforming ‘non-healable’ wounds into healable wounds. Declaration of interest: Randall Wolcott has patented the combination of lactoferrin and xylitol discussed in this article.
A Microscopic and Spectroscopic Study of Interactions between Carbon Nanotubes and a Conjugated Polymer (2002)

Production of stable polymer−nanotube composites depends on good wetting interaction between polymer and nanotube, which is polymer specific, and depends in particular on chain conformation. In this paper, we examine this interaction for a conjugated, semiconducting polymer by a range of microscopic and spectroscopic techniques, to gain a greater understanding of the binding. Several interesting effects are observed, including an order to the interaction between the polymer and nanotube, the tendency of defects in the nanotube structure to nucleate crystal growth, and substantial changes in the spectroscopic behavior of the polymer due to the effect of the nanotubes on polymer conformation. This is substantiated by computational modeling, which demonstrates that these conformational modifications are due to the interaction with the nanotubes.
Filtered multitone modulation for very high-speed digital subscriber lines (2002)

A filter-bank modulation technique called filtered multitone (FMT) and its application to data transmission for very high-speed digital subscriber line technology are described. The proposed scheme leads to significantly lower spectral overlapping between adjacent subchannels than for known multicarrier techniques such as discrete multitone (DMT) or discrete wavelet multitone. FMT modulation mitigates interference due to echo and near-end crosstalk signals, and increases the system throughput and reach. Signal equalization in an FMT receiver is accomplished in the form of per-subchannel symbol-spaced or fractionally spaced linear or decision-feedback equalization. The problem of channel coding for this type of modulation is also addressed, and an approach that allows combined removal of intersymbol-interference via precoding and trellis coding is described. Furthermore, practical design aspects regarding filter-bank realization, initial transceiver training, adaptive equalization, and timing recovery are discussed. Finally, simulation results of the performance achieved by FMT modulation for very high-speed digital subscriber line systems, where upstream and downstream signals are separated by frequency-division duplexing, are presented and compared with DMT modulation.
Needle and catheter navigation using electromagnetic tracking for computer-assisted C-arm CT interventions (2007)

Integrated solutions for navigation systems with CT, MR or US systems become more and more popular for medical products. Such solutions improve the medical workflow, reduce hardware, space and costs requirements. The purpose of our project was to develop a new electromagnetic navigation system for interventional radiology which is integrated into C-arm CT systems. The application is focused on minimally invasive percutaneous interventions performed under local anaesthesia. Together with a vacuum-based patient immobilization device and newly developed navigation tools (needles, panels) we developed a safe and fully automatic navigation system. The radiologist can directly start with navigated interventions after loading images without any prior user interaction. The complete system is adapted to the requirements of the radiologist and to the clinical workflow. For evaluation of the navigation system we performed different phantom studies and achieved an average accuracy of better than 2.0 mm.
Adverse Tissue Reactions to Bioabsorbable Fixation Devices (2000)

Among 2528 patients operated on using pins, rods, bolts, and screws made of polyglycolic acid or polylactic acid, 108 (4.3%) were affected by a clinically significant local inflammatory, sterile tissue reaction. The three most common indications for the use of these fixation devices were a displaced malleolar fracture, a chevron osteotomy for hallux valgus, and a displaced fracture of the radial head. In 107 patients, the reaction was elicited by a polyglycolic acid implant, and in one patient by a polylactic acid implant. The incidences were 5.3% (107 of 2037) and 0.2% (one of 491), respectively. The adverse tissue responses to polyglycolic acid were seen 11 weeks after the operation, on average, whereas the reaction to polylactic acid occurred 4.3 years after fixation of an ankle fracture. The mild reactions consisted of a painful erythematous papule of a few weeks' duration. Those of medium severity had a sinus that discharged remnants of the implant for up to 6 months. In the patients affected by severe reactions, extensive osteolytic lesions developed at the implant tracks. The histopathologic picture was that of a nonspecific foreign body reaction. In four patients with vigorous reactions, an arthrodesis of the wrist or ankle later was necessary because of severe osteoarthritis. Several markers of increased risk of the occurrence of a foreign body reaction were found. These included a poorly vascularized bone section such as scaphoid, use of a quinone dye as an additive in the polymer, and an implant geometry with large surface area (screw versus pin or rod). For polyglycolic acid implants, the risk of an adverse tissue response in a given clinical situation can be estimated from the findings of this study. For slow degrading polymers like polylactic acid, however, the ultimate biocompatibility still is unsettled, and additional clinical research with long followup is required.
Hash Joins and Hash Teams in Microsoft SQL Server (1998)

The query execution engine in Microsoft SQL Server employs hash-based algorithms for inner and outer joins, semi-joins, set operations (such as intersection), grouping, and duplicate removal. The implementation combines many techniques proposed individually in the research literature but never combined in a single implementation, neither in a product nor in a research prototype. One of the paper’s contributions is a design that cleanly integrates most existing techniques. One technique, however, which we call hash teams and which has previously been described only in vague terms, has not been implemented in prior research or product work. It realizes in hash-based query processing many of the benefits of interesting orderings in sort-based query processing. Moreover, we describe how memory is managed in complex and bushy query evaluation plans with multiple sort and hash operations. Finally, we report on the effectiveness of hashing using two very typical database queries, including the performance effects of hash teams.
2000 Fleming Lecture. The origin and evolution of hepatitis viruses in humans (2001)

The spread and origins of hepatitis C virus (HCV) in human populations have been the subject of extensive investigations, not least because of the importance this information would provide in predicting clinical outcomes and controlling spread of HCV in the future. However, in the absence of historical and archaeological records of infection, the evolution of HCV and other human hepatitis viruses can only be inferred indirectly from their epidemiology and by genetic analysis of contemporary virus populations. Some information on the history of the latter may be obtained by dating the time of divergence of various genotypes of HCV, hepatitis B virus (HBV) and the non-pathogenic hepatitis G virus (HGV)/GB virus-C (GBV-C). However, the relatively recent times predicted for the origin of these viruses fit poorly with their epidemiological distributions and the recent evidence for species-associated variants of HBV and HGV/GBV-C in a wide range of non-human primates. The apparent conservatism of viruses over long periods implied by these latter observations may be the result of constraints on sequence change peculiar to viruses with single-stranded genomes, or with overlapping reading frames. Large population sizes and intense selection pressures that optimize fitness may be the factors that set virus evolution apart from that of their hosts.
Six Degree-of-Freedom Haptic Rendering Using Spatialized Normal Cone Search (2005)

This paper describes a haptic rendering algorithm for arbitrary polygonal models using a six degree-of-freedom haptic interface. The algorithm supports activities such as virtual prototyping of complex polygonal models and adding haptic interaction to virtual environments. The underlying collision system computes local extrema in distance between the model controlled by the haptic device and the rest of the scene. The haptic rendering computes forces and torques on the moving model based on these local extrema. The system is demonstrated on models with tens of thousands of triangles and developed in an accessibility application for finding collision-free paths.
Temporal stability of nerve cuff electrode recruitment properties (2002)

The recruitment properties of multiple contact nerve cuff electrodes chronically implanted on the cat sciatic nerve were measured, and the week to week variability was documented. The variability in recruitment properties was greatest between 1 week and 8 weeks post-implant. After 8 weeks the session to session changes were significantly smaller than in the early post-implant period. No trends were observed in the recruitment patterns that would suggest any damage to the nerve occurring during the implant period. These results suggest that the implant is safe and that tissue encapsulation acts to stabilize the cuff electrode and prevent relative movement between the cuff and the nerve trunk. This study, combined with previous studies, demonstrates that multiple contact spiral nerve cuff electrodes can be used in long-term implants to activate discrete regions of peripheral nerve trunks.
Interferon alpha increases the frequency of interferon gamma-producing human CD4+ T cells. (1993)

An increased ratio of T helper type 2 (Th2)- vs Th1-like cells contributes to the immune dysregulation in allergic disease situations and in many chronic infections, including AIDS. Th2-type immune responses are characterized by Th cells that produce increased levels of interleukin-4 (IL-4) and decreased levels of interferon gamma (IFN-gamma). The induction of either a Th1- or a Th2-like phenotype may be critically controlled by the antigen-presenting cells and their cytokines, e.g., IFN-alpha. In this study we have determined the frequencies of potential IL-4- and/or IFN-gamma-producing T cells in the peripheral blood of randomly selected healthy individuals, and analyzed whether IFN-alpha controls IL-4 and/or IFN-gamma production. Purified CD4+ or CD8+ T cells were stimulated for 24 h via the T cell receptor/CD3 complex in the presence or absence of IFN-alpha, and single IL-4- and IFN-gamma-secreting cells were detected in enzyme-linked immunospot assays. In the absence of IFN-alpha, CD4 cells produced IFN-gamma at frequencies of 1:50-300, and produced IL-4 at frequencies of 1:110-<1:100,000. Addition of IFN-alpha during the activation of CD4 cells increased the levels of IFN-gamma mRNA. As a consequence, the numbers of IFN-gamma-producing CD4 cells and the amounts of secreted IFN-gamma increased 10-fold. In contrast, IFN-alpha did not increase the frequency of IL-4-secreting CD4 cells. In the absence of IFN-alpha, addition of exogenous IL-4 to cultures of CD4 cells suppressed IFN-gamma secretion by 70%. However, in the presence of IFN-alpha, IL-4 did not display any suppressive effect. Compared with CD4 cells, CD8 cells produced IFN-gamma more frequently (1:5-10) but IL-4 less frequently (1:5,300 to < 1:100,000). IFN-alpha did not display any effect on the frequency of either IFN-gamma or IL-4 production by CD8 cells. Taken together the results indicate that IFN-alpha increases the frequency of IFN-gamma-secreting CD4 Th cells and antagonizes the suppressive effect of IL-4 on IFN-gamma production. As a consequence, IFN-alpha may favor the induction and maintenance of Th1-like cells and thereby counteract Th2-driven allergic immune responses.
Highly efficient CRISPR/Cas9-mediated transgene knockin at the H11 locus in pigs (2015)

Transgenic pigs play an important role in producing higher quality food in agriculture and improving human health when used as animal models for various human diseases in biomedicine. Production of transgenic pigs, however, is a lengthy and inefficient process that hinders research using pig models. Recent applications of the CRISPR/Cas9 system for generating site-specific gene knockout/knockin models, including a knockout pig model, have significantly accelerated the animal model field. However, a knockin pig model containing a site-specific transgene insertion that can be passed on to its offspring remains lacking. Here, we describe for the first time the generation of a site-specific knockin pig model using a combination of CRISPR/Cas9 and somatic cell nuclear transfer. We also report a new genomic "safe harbor" locus, named pH11, which enables stable and robust transgene expression. Our results indicate that our CRISPR/Cas9 knockin system allows highly efficient gene insertion at the pH11 locus of up to 54% using drug selection and 6% without drug selection. We successfully inserted a gene fragment larger than 9 kb at the pH11 locus using the CRISPR/Cas9 system. Our data also confirm that the gene inserted into the pH11 locus is highly expressed in cells, embryos and animals.