gebhart/patrepeval huggingface dataset:
Metastatic Melanoma Cells in the Sentinel Node Cannot Be Ignored (2009)

These results suggest that patients with submicrometastatic SN disease should not be treated as node-negative, as it appears to represent a more biologically aggressive melanoma, associated with a substantially faster time to recurrence. We cannot agree with recent proposals that patients with very small tumor burden in the SN can be treated as node-negative and be spared completion node dissection.
Antibiotic activity against intraleukocytic Staphylococcus aureus in vitro and in experimental mastitis in mice (1983)

The activities of a range of antibiotics on Staphylococcus aureus organisms that survive within bovine neutrophils in vitro were studied in mice. Cloxacillin, floxacillin, and cephradine failed to kill intracellular staphylococci but increased the organisms' sensitivity to killing by lysostaphin after neutrophil disruption. Fusidate and clindamycin caused an apparent small reduction in viable intraleukocytic S aureus, whereas novobiocin did not demonstrate intracellular activity. Substantial intracellular bactericidal effects were shown in vitro by rifampin and rifamycin SV, even at concentrations in slight excess of the minimum inhibitory concentration. In a mouse model of chronic mastitis, intramammary therapy with rifampin was more effective in reducing viable S aureus in infected glands than was therapy with rifamycin SV.
Isolation and Properties of the DNA of African Swine Fever (ASF) Virus (1976)

African swine fever (ASF) virus was grown either in swine macrophages or in VERO cells and purified free of cell DNA. Virus DNA was isolated from virions as a molecule with a sedimentation coefficient of 60S and a contour of 58 +/- 3 mum. .these two values give a mol. wt. of 102 +/- 5 X 10(6) and 107 +/- 5 X 10(6), respectively, for the genome of ASF virus. Denatured DNA fragments from ASF virus reassociate with a C0t1/2 value of 0-60 +/- 0-05 MS, which compared with the corresponding value for T4 DNA gives for the molecular mass of ASF virus DNA a value of 102 +/- 8 X 10(6) daltons. Only virus DNA is synthesized ASF virus-infected swine macrophages.
UV−Vis Spectroscopy of Iodine Adsorbed on Alkali-Metal-Modified Zeolite Catalysts for Addition of Carbon Dioxide to Ethylene Oxide (1999)

The basicity of alkali-metal-exchanged (Na, K, Cs) zeolites X and Y was probed by UV−vis diffuse reflectance spectroscopy of adsorbed iodine. The observed blue shift in the visible absorption spectrum of adsorbed iodine, compared to gaseous iodine, correlated well with the negative charge on the framework oxygen atoms calculated from the Sanderson electronegativity equalization principle. The blue shifts associated with iodine adsorbed on classical catalytic supports like silica, alumina, and magnesia suggest that the iodine adsorption technique for probing basicity is applicable to a wide variety of solids. Iodine was also adsorbed on X and Y zeolites containing occluded cesium oxide formed by decomposition of impregnated cesium acetate. However, the iodine appeared to irreversibly react on these strongly basic samples, possibly forming an adsorbed triiodide ion. As a complement to the adsorption studies, the activity of alkali-metal-containing zeolites for the base-catalyzed formation of ethylene carbonate from ethylene oxide and carbon dioxide was investigated. Among the ion-exchanged zeolites, the cesium form of zeolite X exhibited the highest activity for ethylene carbonate formation. The catalytic activity of a zeolite containing occluded cesium was even higher than that of a cesium-exchanged zeolite. The presence of water adsorbed in zeolite pores promoted the rate of ethylene carbonate formation for both cesium-exchanged and cesium-impregnated zeolite X.
Beam steering with pulsed two-dimensional transducer arrays (1991)

The major problem facing the development of 2-D arrays for imaging is the complexity arising from the large number of elements anticipated in such transducers. The authors have undertaken a theoretical investigation of the focusing and steering properties of pulsed 2-D arrays to characterize the parameters required for medical imaging, such as element size, spacing, and number of elements. Details of the computational methods employed are presented, as well as a discussion of the steered beam properties of wideband 2-D arrays. The effects of apodization and element cross-coupling on the beam properties of a 2-D transducer array are examined. The beam properties of various sparse arrays with elements randomly distributed over the aperture of the transducer are discussed.
Cytotoxic effects of gemcitabine-containing regimens against human non-small cell lung cancer cell lines which express different levels of p185neu. (1996)

A novel pyrimidine analogue, gemcitabine, has been found to inhibit DNA replication and repair. We speculated that gemcitabine in combination with DNA-damaging agents might be more active against high- than low-p185neu expressing non-small cell lung cancer (NSCLC) cells because the high-p185neu expressors were proposed to posses a more effective DNA repair ability. We therefore compared the combination effects of gemcitabine plus cisplatin, gemcitabine plus etoposide, and cisplatin plus etoposide in a panel of 12 NSCLC cell lines. We also investigated the correlations between the level of p185neu and the cytotoxicities of each single agent and the three combinations. We found that as single agents the cytotoxicities of cisplatin and etoposide but not gemcitabine were significantly correlated with the level of p185neu. In contrast to the tight cross-resistance between cisplatin and etoposide, gemcitabine demonstrated little cross-resistance to either etoposide or cisplatin. Both gemcitabine-containing combinations demonstrated equivalent or more active cytotoxicities compared to cisplatin plus etoposide, with gemcitabine plus cisplatin showing a greater synergistic activity which was effect (dose) dependent. The effect of cisplatin plus etoposide was not p185neu related, whereas gemcitabine-containing regimens, especially gemcitabine plus cisplatin, had a greater cytotoxicity against the high- than the low-p185neu expressors. Our findings indicate that gemcitabine in combination with cisplatin is active against NSCLC cells in vitro. The gemcitabine-cisplatin interaction is more active than the etoposide-cisplatin interaction in cells with high-p185neu expression.
Magnetic resonance imaging. 2nd edition (1987)

This book includes an updated physics section, and expanded coverage of clinical applications of magnetic resonance imaging.
Preparation and characterization of biotinylated red blood cells (1994)

Biotinylation of intact mammalian red blood cells (RBC) was performed either by attachment to the amino groups by means of biotin N-hydrosuccinimide ester (NHS-biotin) or by oxidation of the induced aldehyde groups of the RBC membrane by biotin hydrazide. Comparison of these different procedures showed that biotinylation by NHS-biotin provided the highest cell recovery (> 90%), the binding of approximately 1000 biotin molecules/cell (on mouse RBC) and the 24 h survival in circulation was unaffected. In contrast, biotin hydrazide produced cell recovery in the 5-30% range (depending on the extent of oxidation), an undetectable number of molecules of biotin/cell and negligible 24 h survival. Among the NHS derivatives of biotin, further studies were performed on those containing a spacer arm of 2.2 nm (22 A) [sulphosuccinimidyl-6-(biotinamido)-hexanoate]. In vitro this derivative was similar to, or better than, the NHS-biotin in terms of cell recovery and the number of molecules/cell. In vivo this derivative showed a 24 h circulation survival similar to that of NHS-biotin. Unfortunately, biotin bound with such a spacer arm is lost after a few days of RBC circulation, probably due to plasma biotinidase. Possible applications of biotinylated RBCs include the in vivo measurement of RBC volume, the RBC survival and the delivery of enzyme and antigens.
Steep channel profiles in n/pMOS controlled by boron-doped Si:C layers for continual bulk-CMOS scaling (2009)

Steep channel impurity-profiles formed by Si:C+Si epitaxial growth have been extensively studied. Especially in pMOS, several concerns are solved by boron-doping underneath Si:C layers. Finally, performance improvement realized by steep channel profiles has been demonstrated in both nMOS and pMOS with the same epitaxial channel structure.
The mechanical properties of the human subcalcaneal fat pad in compression. (1990)

The subcalcaneal fat pads of Homo sapiens were subjected to cyclic compressive loading in a materials testing machine. Rates of loading and the absolute loads to which pads were subjected were chosen to simulate the pattern of forces that the pad would be exposed to during the ground contact phase of the running step. Heel pads were found to be resilient, returning approximately 70% of the energy used to deform them. This was modified little by changes in loading frequency. A reduction of temperature from 37 degrees C to 10 degrees C produced a small, but significant, increase in the percentage energy dissipation.
The Epidermal Growth Factor Receptor Regulates Interaction of the Human DF3/MUC1 Carcinoma Antigen with c-Src and β-Catenin (2001)

The DF3/MUC1 mucin-like, transmembrane glycoprotein is aberrantly overexpressed in most human carcinomas. The MUC1 cytoplasmic domain interacts with the c-Src tyrosine kinase and thereby increases binding of MUC1 and beta-catenin. In the present work, coimmunoprecipitation studies demonstrate that MUC1 associates constitutively with the epidermal growth factor receptor (EGF-R) in human ZR-75-1 breast carcinoma cells. Immunofluorescence studies show that EGF-R and MUC1 associate at the cell membrane. We also show that the activated EGF-R phosphorylates the MUC1 cytoplasmic tail on tyrosine at a YEKV motif that functions as a binding site for the c-Src SH2 domain. The results demonstrate that EGF-R-mediated phosphorylation of MUC1 induces binding of MUC1 to c-Src in cells. Moreover, in vitro and in vivo studies demonstrate that EGF-R increases binding of MUC1 and beta-catenin. These findings support a novel role for EGF-R in regulating interactions of MUC1 with c-Src and beta-catenin.
Texas Red-X and Rhodamine Red-X, New Derivatives of Sulforhodamine 101 and Lissamine Rhodamine B with Improved Labeling and Fluorescence Properties (1996)

Texas Red sulfonyl chloride (TR-SC) and Lissamine rhodamine B sulfonyl chloride (LRB-SC) are popular dyes often used to prepare red fluorescent conjugates that are useful second labels in combination with fluorescein. Unfortunately, being sulfonyl chloride derivatives, both are unstable to moisture during storage and prone to hydrolysis in the conjugation reaction. Their instability causes the percentage of reactive dye to vary from lot to lot and requires use of low temperatures and a relatively high pH to optimize conjugation efficiency. Succinimidyl esters of the aminohexanoic acid sulfonamides of both dyes have been prepared, which are designated Texas Red-X succinimidyl ester (TR-X-SE) and Rhodamine Red-X succinimidyl ester, respectively. Their spectral properties are similar to those of their sulfonyl chloride analogs; moreover, incorporation of the succinimidyl ester at the end of the aliphatic chain spacer facilitates conjugation, decreases precipitation of proteins during conjugation and storage, and usually increases the fluorescence yield of the conjugate. Comparison of the rate of hydrolysis of TR-SC with that of TR-X-SE shows that, while the former was completely hydrolyzed within 5 min by exposure to water, TR-X-SE retains most of its reactivity for more than an hour. The reactivity of both new derivatives is high between pH 7.5 and 8.5, allowing conjugation of proteins that do not tolerate the high pH required for reaction with sulfonyl chlorides. In addition, Texas Red maleimides and haloacetamides containing spacer groups were prepared for labeling sulfhydryl groups. A Texas Red-X derivative of phalloidin has also been prepared, and its use for labeling F-actin has been characterized.
Cilia-based flow network in the brain ventricles (2016)

Cerebrospinal fluid conveys many physiologically important signaling factors through the ventricular cavities of the brain. We investigated the transport of cerebrospinal fluid in the third ventricle of the mouse brain and discovered a highly organized pattern of cilia modules, which collectively give rise to a network of fluid flows that allows for precise transport within this ventricle. We also discovered a cilia-based switch that reliably and periodically alters the flow pattern so as to create a dynamic subdivision that may control substance distribution in the third ventricle. Complex flow patterns were also present in the third ventricles of rats and pigs. Our work suggests that ciliated epithelia can generate and maintain complex, spatiotemporally regulated flow networks.
Dietary omega 3 fatty acids and endothelium-dependent relaxations in porcine coronary arteries (1989)

Dietary supplementation with cod-liver oil significantly augments endothelium-dependent relaxations in porcine coronary arteries. The present study was designed to examine the effect of dietary administration of omega 3 polyunsaturated fatty acids (mainly eicosapentaenoic acid, the major component of fish oil) on endothelium-dependent relaxations in porcine coronary arteries. Male Yorkshire pigs were maintained 4 wk on a regular diet with or without supplementation with purified eicosapentaenoic acid (3.5 g/day) and docosahexaenoic acid (1.5 g/day). Endothelium-dependent relaxations were examined in vitro. In rings from the treated group, endothelium-dependent relaxations were augmented in response to bradykinin, serotonin, and ADP, but not to the calcium ionophore A23187. These augmentations were not altered by indomethacin but were significantly inhibited by methylene blue, an inhibitor of guanylate cyclase. In the treated group, endothelium-dependent relaxations to aggregating platelets also were significantly augmented; platelet-induced contractions of quiescent rings were inhibited more by the presence of the endothelium than in arteries from the control group. Bioassay experiments demonstrated that the release of endothelium-derived relaxing factor(s) by bradykinin and relaxations of the vascular smooth muscle to the factor(s) were greater in arteries from the treated group. These observations indicate that dietary omega 3 polyunsaturated fatty acids augment receptor-operated endothelium-dependent relaxations, partly due to the augmented release of endothelium-derived relaxing factor(s) and partly due to the augmented relaxation of the vascular smooth muscle to the factor(s).
Comparison of flow cytometry and laser scanning cytometry for the intracellular evaluation of adenoviral infectivity and p53 protein expression in gene therapy (1998)

The determination of recombinant adenoviral (rAd) infectivity and p53 protein expression is important for the evaluation of rAd vectors containing the p53 gene (rAd-CMV-p53) for gene therapy. We have previously reported that rAd5-CMV-p53 vectors can be assessed for infectivity and concomitant p53 protein expression in single- and two-color assays using intracellular staining methodology and flow cytometric analysis. We have compared the flow cytometry-based assays for rAd infectivity (hexon protein) and p53 protein expression with the new slide-based laser scanning cytometry (LSC). We report that LSC analysis of rAd-CMV-p53-infected human 293 cells correlated very well with flow cytometric analysis across a wide range of viral infectivity for both infectivity assessment (r2 = 0.97) and p53 protein expression (r2 = 0.96). Absolute values for infective titer and p53 protein expression titer from an rAd5-CMV-p53 production batch were similar and within experimental error with the two different analytical methods. Finally, bivariate format analysis of rAd-CMV-p53-infected cells revealed comparable results between LSC and flow cytometric analysis. LSC is a reliable and useful tool for the intracellular staining for adenoviral hexon protein expression for determining infectivity and for p53 protein expression from an expressed p53 transgene.
‘Shotgun DNA synthesis’ for the high-throughput construction of large DNA molecules (2012)

We developed a highly scalable 'shotgun' DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments, and tagged with 20 bp degenerate barcode primer pairs. An optimal set of error-free fragments were identified by high-throughput DNA sequencing, selectively amplified using the barcode sequences, and successfully assembled into the target gene cluster.
Engineering Enzyme Specificity by "Substrate-Assisted Catalysis" (1987)

A novel approach to engineering enzyme specificity is presented in which a catalytic group from an enzyme is first removed by site-directed mutagenesis causing inactivation. Activity is then partially restored by substrates containing the missing catalytic functional group. Replacement of the catalytic His with Ala in the Bacillus amyloliquefaciens subtilisin gene (the mutant is designated His64Ala) by site-directed mutagenesis reduces the catalytic efficiency (kcat/Km) by a factor of a million when assayed with N-succinyl-L-Phe-L-Ala-L-Ala-L-Phe-p-nitroanilide (sFAAF-pNA). Model building studies showed that a His side chain at the P2 position of a substrate bound at the active site of subtilisin could be virtually superimposed on the catalytic His side chain of this serine protease. Accordingly, the His64Ala mutant hydrolyzes a His P2 substrate (sFAHF-pNA) up to 400 times faster than a homologous Ala P2 or Gln P2 substrate (sFAAF-pNA or sFAQF-pNA) at pH 8.0. In contrast, the wild-type enzyme hydrolyzes these three substrates with similar catalytic efficiencies. Additional data from substrate-dependent pH profiles and hydrolysis of large polypeptides indicate that the His64Ala mutant enzyme can recover partially the function of the lost catalytic histidine from a His P2 side chain on the substrate. Such "substrate-assisted catalysis" provides a new basis for engineering enzymes with very narrow and potentially useful substrate specificities. These studies also suggest a possible functional intermediate in the evolution of the catalytic triad of serine proteases.
Free and Supported Phosphorus Ylides as Strong Neutral Brønsted Bases (1999)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTFree and Supported Phosphorus Ylides as Strong Neutral Brønsted BasesStéphanie Goumri-Magnet, Olivier Guerret, Heinz Gornitzka, Jean Bernard Cazaux, Dennis Bigg, Francisco Palacios, and Guy BertrandView Author Information Laboratoire de Chimie de Coordination, 205 route de Narbonne, 31077 Toulouse Cedex, France, Expansia S. A., route d'Avignon, BP 6, 30390 Aramon Cedex, France, Institut Henri Beaufour, 17, avenue Henri descartes, 92350 Le Plessis Robinson, France, and Facultad de Farmacia, Universidad del Pais Vasco, Aparrado 450, 01080 Vitoria, Spain Cite this: J. Org. Chem. 1999, 64, 10, 3741–3744Publication Date (Web):April 30, 1999Publication History Received3 November 1998Published online30 April 1999Published inissue 1 May 1999https://pubs.acs.org/doi/10.1021/jo9822030https://doi.org/10.1021/jo9822030brief-reportACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views1055Altmetric-Citations22LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Deprotonation,Nuclear magnetic resonance spectroscopy,Organic polymers,Polymers,Salts Get e-Alerts
BigHand2.2M Benchmark: Hand Pose Dataset and State of the Art Analysis (2017)

In this paper we introduce a large-scale hand pose dataset, collected using a novel capture method. Existing datasets are either generated synthetically or captured using depth sensors: synthetic datasets exhibit a certain level of appearance difference from real depth images, and real datasets are limited in quantity and coverage, mainly due to the difficulty to annotate them. We propose a tracking system with six 6D magnetic sensors and inverse kinematics to automatically obtain 21-joints hand pose annotations of depth maps captured with minimal restriction on the range of motion. The capture protocol aims to fully cover the natural hand pose space. As shown in embedding plots, the new dataset exhibits a significantly wider and denser range of hand poses compared to existing benchmarks. Current state-of-the-art methods are evaluated on the dataset, and we demonstrate significant improvements in cross-benchmark performance. We also show significant improvements in egocentric hand pose estimation with a CNN trained on the new dataset.
NOW: ANTI-COLLISION RADAR FOR YOUR CAR (1986)

Cholinergic neurons in the basal forebrain and the brainstem form extensive projections to a number of thalamic nuclei. Activation of cholinergic afferents during distinct behavioral states can regulate neuronal firing, transmitter release at glutamatergic and GABAergic synapses, and synchrony in thalamic networks, thereby controlling the flow of sensory information. These effects are thought to be mediated by slow and persistent increases in extracellular ACh levels, resulting in the modulation of populations of thalamic neurons over large temporal and spatial scales. However, the synaptic mechanisms underlying cholinergic signaling in the thalamus are not well understood. Here, we demonstrate highly reliable cholinergic transmission in the mouse thalamic reticular nucleus (TRN), a brain structure essential for sensory processing, arousal, and attention. We find that ACh release evoked by low-frequency stimulation leads to biphasic excitatory-inhibitory (E-I) postsynaptic responses, mediated by the activation of postsynaptic α4β2 nicotinic ACh receptors (nAChRs) and M2 muscarinic ACh receptors (mAChRs), respectively. In addition, ACh can bind to mAChRs expressed near cholinergic release sites, resulting in autoinhibition of release. We show that the activation of postsynaptic nAChRs by transmitter release from only a small number of individual axons is sufficient to trigger action potentials in TRN neurons. Furthermore, short trains of cholinergic synaptic inputs can powerfully entrain ongoing TRN neuronal activity. Our study demonstrates fast and precise synaptic E-I signaling mediated by ACh, suggesting novel computational mechanisms for the cholinergic control of neuronal activity in thalamic circuits.