gebhart/patrepeval huggingface dataset:
Reducing regulatory barriers to private - sector participation in Latin America's water and sanitation services (1994)

The lack of an appropriate regulatory environment is a principal factor behind inadequate water and sanitation services in many parts of Latin America. Many governments recognize the need to improve cost recovery and accountability in services - and increasingly see private sector participation as a tool for improving efficiency and attracting commercial sources of investment finance. Consultants interviewed representatives of private companies that recently contended for contracts to provide water and sanitation services in four Latin American cities (Buenos Aires, Caracas, Mexico City, and Santiago). These private operators identify the regulatory conditions they look for deciding whether to participate in a bid. On the basis of the interviews, the authors identified nine conditions. (1) Specify key terms and conditions of regulation in the contract, leaving little discretionary power to the regulating authority. In particular, specify the key aspects of regulation (such as price, quantity, and quality) in the contract. (2) Spell out credible procedures for the fair resolution of disagreements about contractual or regulatory matters. (3) Carefully specify credible technical objectives which the contractor will be expected to achieve under the contract. (4) See that government tariff policies support the principle of cost recovery for water services - and that tariff adjustment formulas adequately reflect changes in costs, inflation, and the exchange rate. (5) If historical collection rates do not indicate consumers'willingness to pay for services such as tariffs that reflect the cost of service, allow an adequate period of time to phase in higher tariffs - and give the operator adequate protection from nonpayers (either the right to cut off service or recourse to another source of payment). (6) Review public works law, contract law, and accounting practices and, if necessary, amend them in advance to ensure that they accommodate and protect any long-term investments foreseen under build-own-transfer or concession-type arrangements. (7) Eliminate unnecessary and bureaucratic administrative requirements that make bidding expensive. (8) Make a contract and expected profits big enough to warrant the high fixed cost of bidding. (9) Provide the education and outreach needed to inform consumers and secure the support of labor interests. In addition, the firms interviewed said that host countries would be better able to attract private-sector providers if they: (a) used reputable outside technical, legal, and financial advisors; (b) allowed local and foreign banks that finance investments to review and comment on proposed contracts and participate in negotiations; and (c) reduced the cost of bidding for small contracts.
Quantitative Detection of Telomerase Components by Real-Time, Online RT-PCR Analysis with the LightCycler (2003)

Telomeres are specialized DNA/protein structures, located at the ends of eukaryotic chromosomes, that consist of small, tandemly repeated DNA sequences. They have an essential role in the stable maintenance of the eukaryotic chromosome by preventing nucleolytic degradation, end-to-end fusion, irregular recombination, and other events that are normally lethal to a cell (1). In most normal tissues telomeres progressively shorten with each replication cycle as a result of the end-replication problem (2). Because germline cells, stem cells, and especially tumor cells exhibit a prolonged or even infinite life span, it was proposed that these cells must possess a particular mechanism for maintaining telomere length (3).
Specific metaphase and interphase detection of the breakpoint region in 8q24 of burkitt lymphoma cells by triple‐color fluorescence in situ hybridization (1992)

Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma cell line J1 was used for the specific delineation of this breakpoint in individual tumor cells. With this approach, tumor-specific breakpoints in translocation chromosomes can be detected at all stages of the cell cycle with high specificity.
Diagnostic accuracy of 18F amyloid PET tracers for the diagnosis of Alzheimer’s disease: a systematic review and meta-analysis (2015)

Imaging or tissue biomarker evidence has been introduced into the core diagnostic pathway for Alzheimer's disease (AD). PET using (18)F-labelled beta-amyloid PET tracers has shown promise for the early diagnosis of AD. However, most studies included only small numbers of participants and no consensus has been reached as to which radiotracer has the highest diagnostic accuracy. First, we performed a systematic review of the literature published between 1990 and 2014 for studies exploring the diagnostic accuracy of florbetaben, florbetapir and flutemetamol in AD. The included studies were analysed using the QUADAS assessment of methodological quality. A meta-analysis of the sensitivity and specificity reported within each study was performed. Pooled values were calculated for each radiotracer and for visual or quantitative analysis by population included. The systematic review identified nine studies eligible for inclusion. There were limited variations in the methods between studies reporting the same radiotracer. The meta-analysis results showed that pooled sensitivity and specificity values were in general high for all tracers. This was confirmed by calculating likelihood ratios. A patient with a positive ratio is much more likely to have AD than a patient with a negative ratio, and vice versa. However, specificity was higher when only patients with AD were compared with healthy controls. This systematic review and meta-analysis found no marked differences in the diagnostic accuracy of the three beta-amyloid radiotracers. All tracers perform better when used to discriminate between patients with AD and healthy controls. The sensitivity and specificity for quantitative and visual analysis are comparable to those of other imaging or biomarker techniques used to diagnose AD. Further research is required to identify the combination of tests that provides the highest sensitivity and specificity, and to identify the most suitable position for the tracer in the clinical pathway.
BCG induced CD4+ cytotoxic T cells from BCG vaccinated healthy subjects: relation between cytotoxicity and suppression in vitro. (1987)

Mycobacterial antigen specific cytotoxic T cells killing antigen-pulsed antigen presenting cells (APC) were induced from peripheral blood mononuclear cells (PBMC) of BCG-vaccinated healthy subjects after activation in vitro with BCG. CD8+ depleted cells were as effective as PBMC, indicating that CD4+ cells play a dominant role in this phenomenon. CD4+ T cell clones raised against BCG also exhibited mycobacterial antigen specific cytotoxicity and suppressed BCG-driven selfproliferation. However, the same clones could either suppress or enhance the proliferation of other T cell clones from the same subject. The possible function in vivo of the cytotoxicity mediated by CD4+ T cells is discussed.
Separation of catechin compounds from different teas (2006)

Catechin compounds from Korean and Chinese green tea, and pu-erh, Indian black, Longjing, Tieguanyin, Bamboo, Jasmine, Oolong, Flower, Red teas, as potential anticancer and antioxidant components, were target material in this work. After extracting the green tea with water at 50 degrees C for 4 h, the extract was partitioned with water/chloroform, which was best suited to remove caffeine impurity from the extract. Further, the resulting extract was partitioned with water/ethyl acetate to deeply purify the five catechin compounds epigallocatechin, (+) catechin, epicatechin, epigallocatechin gallate and epicatechin gallate. The extracted samples were analyzed by reversed-phase high performance liquid chromatography. The mobile phase applied was the binary system of A (water/acetic acid, 100/0.1 vol%) and B (acetonitrile/acetic acid 100/0.1 vol%) from 90:10 to 70:30 (A:B vol%) in a linear gradient over 30 min time. The amount of catechin compounds extracted from Chinese green tea was 114.65% higher than from the Korean green tea. Comparing various tea sorts, the green teas contained more than 1.7 times of the five catechin compounds contained in other teas.
Validation of a simplified grading of Gram stained vaginal smears for use in genitourinary medicine clinics (2002)

This simplified assessment of Gram stained smears can be used as an alternative to Amsel's criteria and is more applicable for use in busy GUM clinics.
Nicotinic Acid Side Effects and Toxicity: A Review (1970)

The author reviews the literature on the possible toxicity of nicotinic acid, whose therapeutic value in the treatment of schizophrenia is still in dispute. While the drug appears to be relatively harmless, caution in prescribing it is advised, especially for women of childbearing age and for patients with a history of ulcer, gout, diabetes, or liver disease.
Hemostatic Efficacy of Two Advanced Dressings in an Aortic Hemorrhage Model in Swine (2005)

Both chitosan dressing and fibrin sealant dressing stopped initial arterial bleeding that could not be controlled by the standard army field dressing. However, although the fibrin sealant dressing secured hemostasis for up to 4 days, the chitosan dressing consistently failed within 2 hours after application. There may be a risk of rebleeding for high-pressure arterial wounds treated with chitosan dressings, particularly in situations where definitive care is delayed substantially.
Probing Hot-Electron Dynamics at Surfaces with a Cold Scanning Tunneling Microscope (1999)

We report on a novel approach to measure the phase relaxation length and femtosecond lifetime of hot quasiparticles on metal surfaces. A 4 K scanning tunneling microscope has been used to study the spatial decay of interference patterns in the local density of states for surface state electrons on Ag(111) and Cu(111). This decay is governed by inelastic electron-electron scattering. We find a $({E\ensuremath{-}E}_{F}{)}^{\ensuremath{-}2}$ energy dependence of the lifetimes for both Ag and Cu, and our values are comparable to the corresponding bulk electron lifetimes. This indicates that electron-electron interaction of hot surface state electrons with the Fermi sea is dominated by the underlying bulk electrons.
Adenovirus‐mediated gene transfer to murine retinal cells in vitro and in vivo (1994)

Adenovirus-mediated gene transfer to retinal cells was evaluated using the replication-defective recombinant adenovirus vector Ad2/CMVlacZ-1 (coding for beta-galactosidase) both in an in vitro murine culture model and in vivo in adult mice. In vitro, no difference in infectability of neuronal and glial cells was observed, and 50% of neurons expressed the exogenous gene at low viral concentration (10 pfu/cell). In vivo, intraocular injection of 3 x 10(6) pfu Ad2/CMVlacZ-1 resulted in expression of the transferred beta-galactosidase gene in retinal pigment epithelium and ganglion cells. These results demonstrate that Ad2/CMVlacZ-1 is an effective vector for gene transfer into retinal cells.
Role of the β‐amyloid protein in Alzheimer's disease (1995)

A major histopathological hallmark of Alzheimer's disease (AD) is the presence of amyloid deposits in the parenchyma of the amygdala, hippocampus, and neocortex. The principal component of amyloid is the beta-amyloid protein (A beta), a 39-43 amino acid peptide composed of a portion of the transmembrane domain and the extracellular domain of the amyloid precursor protein (APP). APP occurs as several A beta-containing isoforms of 695, 751, and 770 amino acids, with the latter two APP containing a domain that shares structural and functional homologies with Kunitz serine protease inhibitors. In cultured cells, APP mature through the constitutive secretory pathway, and some cell surface-bound APP are cleaved by an enzyme, designated as alpha-secretase, within the A beta domain, an event that precludes A beta amyloidogenesis. Several studies have delineated two additional pathways of APP processing: first, an endosomal/lysosomal pathway generates a complex set of APP-related membrane-bound fragments, some of which contain the entire A beta sequence; and, second, by mechanisms which are not fully understood, A beta 1-40 is secreted into the conditioned medium in vitro and is present in cerebrospinal fluid in vivo. The intracellular sites of enzymes responsible for proteolytic cleavage at the NH2 and COOH termini of A beta, termed gamma- and beta-secretase, respectively, have not been identified. Finally, recent molecular genetic investigations have identified a variety of mutations in APP that segregate with early-onset familial AD and with hereditary cerebral hemorrhage with amyloid, Dutch type (HCHWA-D). Several of these mutations appear to influence APP processing and result in the production of higher levels or longer A beta-related peptides that are inherently more fibrillogenic. Although a variety of lines of evidence implicates APP/A beta in AD, the mechanisms by which A beta influences the biology and vulnerability of neural cells are not fully understood but are very active areas of investigation. This review focuses on the present state of our understanding of APP and A beta in the context of AD.
Structural Characterization of the Degradation Products of a Minor Natural Sweet Diterpene Glycoside Rebaudioside M under Acidic Conditions (2014)

Degradation of rebaudioside M, a minor sweet component of Stevia rebaudiana Bertoni, under conditions that simulated extreme pH and temperature conditions has been studied. Thus, rebaudioside M was treated with 0.1 M phosphoric acid solution (pH 2.0) and 80 °C temperature for 24 h. Experimental results indicated that rebaudioside M under low pH and higher temperature yielded three minor degradation compounds, whose structural characterization was performed on the basis of 1D (1H-, 13C-) &amp; 2D (COSY, HSQC, HMBC) NMR, HRMS, MS/MS spectral data as well as enzymatic and acid hydrolysis studies.
Tandem Synthesis of Core−Shell Brush Copolymers and Their Transformation to Peripherally Cross-Linked and Hollowed Nanostructures (2006)

Core-shell brush copolymers were prepared on the basis of a tandem synthetic strategy and used as single molecular templates for the preparation of polymeric nanomaterials. An alkoxyamine-functionalized norbornene monomer was prepared and then polymerized by ring-opening metathesis polymerization. The well-defined polymer (Mn = 122 kDa, Mw/Mn = 1.13) contained one alkoxyamine functionality per repeat unit and was then used as a polyfunctional macroinitiator for sequential nitroxide-mediated radical polymerizations of isoprene and tert-butyl acrylate. The resulting well-defined brush copolymer (Mn = 1410 kDa, Mw/Mn = 1.23) was transformed to an amphiphilic core-shell brush copolymer comprising poly(isoprene)-b-poly(acrylic acid) grafts by hydrolysis. Subsequent cross-linking of the poly(acrylic acid) block segments afforded peripherally cross-linked brush copolymer nanostructures, which served, finally, as templates for hollowed nanoscale frameworks by ozonolysis of the poly(isoprene)-based cores. Each transformation led to dramatic changes in the nanoscale composition and structure which were detected by combinations of spectroscopic measurements, atomic force microscopy imaging in the solid state, and/or dynamic light-scattering characterization in aqueous solution.
What is this thing called pain? (2010)

To paraphrase Cole Porter's famous 1926 song, "What is this thing called pain? This funny thing called pain, just who can solve its mystery?" Pain, like love, is all consuming: when you have it, not much else matters, and there is nothing you can do about it. Unlike love, however, we are actually beginning to tease apart the mystery of pain. The substantial progress made over the last decade in revealing the genes, molecules, cells, and circuits that determine the sensation of pain offers new opportunities to manage it, as revealed in this Review series by some of the foremost experts in the field.
Gain and loss of function of ALS-related mutations of TARDBP (TDP-43) cause motor deficits in vivo (2009)

TDP-43 has been found in inclusion bodies of multiple neurological disorders, including amyotrophic lateral sclerosis, frontotemporal dementia, Parkinson's disease and Alzheimer's disease. Mutations in the TDP-43 encoding gene, TARDBP, have been subsequently reported in sporadic and familial ALS patients. In order to investigate the pathogenic nature of these mutants, the effects of three consistently reported TARDBP mutations (A315T, G348C and A382T) were tested in cell lines, primary cultured motor neurons and living zebrafish embryos. Each of the three mutants and wild-type (WT) human TDP-43 localized to nuclei when expressed in COS1 and Neuro2A cells by transient transfection. However, when expressed in motor neurons from dissociated spinal cord cultures these mutant TARDBP alleles, but less so for WT TARDBP, were neurotoxic, concomitant with perinuclear localization and aggregation of TDP-43. Finally, overexpression of mutant, but less so of WT, human TARDBP caused a motor phenotype in zebrafish (Danio rerio) embryos consisting of shorter motor neuronal axons, premature and excessive branching as well as swimming deficits. Interestingly, knock-down of zebrafisfh tardbp led to a similar phenotype, which was rescued by co-expressing WT but not mutant human TARDBP. Together these approaches showed that TARDBP mutations cause motor neuron defects and toxicity, suggesting that both a toxic gain of function as well as a novel loss of function may be involved in the molecular mechanism by which mutant TDP-43 contributes to disease pathogenesis.
Determination of Feasibility and Utility of Microscope-Integrated Optical Coherence Tomography During Ophthalmic Surgery (2015)

The DISCOVER study demonstrates the feasibility of real-time iOCT with a microscope-integrated iOCT system for ophthalmic surgery. The information gained from iOCT appears to allow surgeons to assess subtle details in a unique perspective from standard en face visualization, which can affect surgical decision making some of the time, although the effect of these changes in decision making on outcomes remains unknown. A prospective randomized masked trial is needed to confirm these results.
Efficient Collision Detection of Complex Deformable Models using AABB Trees (1997)

Abstract We present a scheme for exact collision detection between complex models undergoing rigid motion and deformation. The scheme relies on a hierarchical model representation using axis-aligned bounding boxes (AABBs). Recent work has shown that AABB trees are slower than oriented bounding box (OBB) trees for performing overlap tests. In this paper, we describe a way to speed up overlap tests between AABBs, such that for collision detection of rigid models, the difference in performance between the two representations is greatly reduced. Furthermore, we show how to update an AABB tree quickly as a model is deformed. We thus find AABB trees to be the method of choice for collision detection of complex models undergoing deformation. In fact, because they are not much slower to test, are faster to build, and use less storage than OBB trees, AABB trees might be a reasonable choice for rigid models as well.
ChemInform Abstract: Comparative Study of Selected Reagents for Carboxyl Activation. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Layered penumbrae: an effective 3D feedback technique (2002)

Shadows serve as effective cues for a user to recognize spatial relations among objects in 3D space. However simple shadows typically used in 3D interfaces are often insufficient for the purpose, and rendering of shadows with real penumbra are not only too costly for interactive interfaces, but also do not enhance their capabilities as spatial cues. Our proposal, layered penumbra is a new interactive feedback technique that overcomes these deficiencies; it positively enhances the user's perception of spatial relations, looks natural to the eye, and furthermore can be rendered fast enough for interactive 3D interfaces. The layered penumbra has three layers (umbra, penumbra, and the middle layer) separated distinctly at their perimeters. A layered penumbra is drawn as a series of simple orthogonal projections of an object locally magnified/shrunken.
Compact fiber lasers for efficient high-power generation (2006)

We report measurements of the performance of mode-filtered, Yb-doped, double-clad fiber amplifiers (30 &#956;m core diameter) seeded with passively Q-switched, Nd:YAG microchip lasers operating at 1 kHz repetition rate and pulse durations of 0.38 ns and 2.3 ns. The amplified pulses were fully characterized spectrally, temporally, and spatially. The output beam quality was diffraction-limited (M<sup>2</sup> < 1.2). With the 0.38 ns seed laser, we obtained a maximum peak power of 1.27 MW, corresponding to a peak in-fiber irradiance of 440 GW/cm2. With the 2.3 ns seed laser, we obtained a maximum pulse energy of 1.1 mJ, corresponding to an in-fiber fluence of 410 J/cm<sup>2</sup>. This irradiance and fluence are the highest observed, to our knowledge, for ns-duration pulses, which bodes well for further power scaling with largercore fibers. Nonlinear processes resulted in spectral broadening and distortion of the pulse temporal profiles. At 1.27 MW peak power, the linewidth containing 80% of the pulse energy was 0.55 nm, and the linewidth at 1.05 mJ pulse energy was 1.0 nm. The experimental measurements were compared with the initial results of a pulse-amplification model containing no adjustable parameters. The model predictions are in excellent agreement with the measured pulse energies and small-signal gains. The laser systems employed a simple architecture, consisting of passively Q-switched oscillators; single-stage, single-pass, cw-pumped amplifiers; conventional, solid fibers; and < 6 cm coil diameters. This approach is suitable for use in practical applications requiring compact, rugged, and efficient laser sources.
From charity to social work: Mary E. Richmond and the creation of an American profession (2004)

Examiantion of the development of modern social work from its roots in late 19th Century charity through the 1920's, focusing on the life and leadership of Mary E. Richmond. Highlights the important role of charity organization philosophy in the transformation from voluntary charity to professional social work.
Estimates of forest canopy height and aboveground biomass using ICESat (2005)

Exchange of carbon between forests and the atmosphere is a vital component of the global carbon cycle. Satellite laser altimetry has a unique capability for estimating forest canopy height, which has a direct and increasingly well understood relationship to aboveground carbon storage. While the Geoscience Laser Altimeter System (GLAS) onboard the Ice, Cloud and land Elevation Satellite (ICESat) has collected an unparalleled dataset of lidar waveforms over terrestrial targets, processing of ICESat data to estimate forest height is complicated by the pulse broadening associated with large‐footprint, waveform‐sampling lidar. We combined ICESat waveforms and ancillary topography from the Shuttle Radar Topography Mission to estimate maximum forest height in three ecosystems; tropical broadleaf forests in Brazil, temperate broadleaf forests in Tennessee, and temperate needleleaf forests in Oregon. Final models for each site explained between 59% and 68% of variance in field‐measured forest canopy height (RMSE between 4.85 and 12.66 m). In addition, ICESat‐derived heights for the Brazilian plots were correlated with field‐estimates of aboveground biomass (r 2 = 73%, RMSE = 58.3 Mgha −1 ).