gebhart/patrepeval huggingface dataset:
Conceptual Design for Configuration and Reconfiguration of Customizable Products (2000)

The development and maintenance of product configuration systems is faced with increasing challenges caused by the growing complexity of the underlying knowledge bases. Effective knowledge acquisition is needed since configurator development time is strictly limited, i.e. the product and the corresponding configuration system have to be developed in parallel. In this paper we show how to employ a standard design language (Unified Modeling Language UML) in order to represent the configuration knowledge on a conceptual level. The two constituent parts of the conceptual configuration model are the component model and a set of corresponding functional architectures defining which requirements can be imposed to the product. The conceptual configuration model is translated into an executable logic representation. Using this representation we show how to employ model-based diagnosis for diagnosing a faulty configuration knowledge base, infeasible requirements, and for reconfiguring old configurations.
Leakage power reduction for clock gating scheme on PD-SOI (2004)

This paper presents a technique for reducing leakage power of the circuits employing a clock gating scheme on Partially Depleted Silicon On Insulator (PD-SOI). To reduce leakage power while a local clock is disabled, V/sub th/ of each transistor is dynamically controlled by body biasing corresponding to the mode of the local clock. Using PD-SOI is the key to control V/sub th/ within one clock cycle by forward biasing, where V/sub th/ without biasing is designed higher than usual to reduce leakage power. The SPICE simulation results have shown that the proposed technique reduces leakage power by 82% with small area penalty.
Characterization of human interleukin 2 derived from Escherichia coli (1985)

Interleukin 2 isolated from Escherichia coli cells expressing the human interleukin gene has been characterized. The observed properties of the protein have been compared with those properties which can be deduced from the DNA sequence alone and the published properties of natural human interleukin 2. The purified E. coli-derived interleukin 2 is a monomeric protein of Mr 15 000 with a sedimentation velocity of 1.86S. The amino acid composition of the protein and isoelectric point (7.7) are consistent with that part of the translated DNA sequence of the gene corresponding to the mature protein. A single disulphide bridge was identified between Cys-58 and Cys-105. C.d. suggested that interleukin 2 is predominantly alpha-helical in secondary structure. The E. coli-derived protein differed from natural interleukin 2 in the presence of N-terminal methionine and also in the absence of a carbohydrate moiety. Removal of the coding region for the first three amino acids of the natural interleukin 2 protein sequence (Ala-Pro-Thr) by site-specific mutagenesis resulted in a protein with N-terminal serine. The possibility that the specificity of the E. coli ribosomal methionine aminopeptidase may not recognize the sequence NH2-Met-Xaa-Pro is discussed (where Xaa is any amino acid residue).
Arylboronic Acids. VIII. Reactions of Boronophthalide1 (1964)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTArylboronic Acids. VIII. Reactions of Boronophthalide1R. R. Haynes and H. R. SnyderCite this: J. Org. Chem. 1964, 29, 11, 3229–3233Publication Date (Print):November 1, 1964Publication History Published online1 May 2002Published inissue 1 November 1964https://pubs.acs.org/doi/10.1021/jo01034a025https://doi.org/10.1021/jo01034a025research-articleACS PublicationsRequest reuse permissionsArticle Views490Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Convenient Syntheses of 7-Hydroxy-1-(hydroxymethyl)- 3-(thymin-1-yl)-2,5-dioxabicyclo[2.2.1]heptanes:  α-l-Ribo- and α-l-Xylo-Configured LNA Nucleosides (2000)

Synthesis of the diastereoisomeric LNA (locked nucleic acid) nucleosides 1-(2-O,4-C-methylene-alpha-L-ribofuranosyl)thymine (6) and 1-(2-O,4-C-methylene-alpha-L-xylofuranosyl)thymine (13) are reported via convenient reaction cascades from di-O-p-toluenesulfonyl and tri-O-methanesulfonyl nucleoside derivatives 3, 7, and 10.
Increased activity of membrane-associated nucleoside diphosphate kinase and inhibition of cAMP synthesis in failing human myocardium (2001)

The amount and activity of nucleoside diphosphate kinase are increased in sarcolemmal membranes of failing human myocardium, resulting in a substantial receptor-independent inhibition of adenylyl cyclase activity.
Activated B cells express receptors for, and proliferate in response to, pure interleukin 2. (1984)

In this study we investigated whether interleukin 2 (IL-2) acts on B cell proliferation and whether activated B cells express IL-2 receptors. First, the functional activity of immunoaffinity-purified or recombinant human IL-2 was studied in a B blast assay using positively selected murine surface Ig-positive cells that had been activated by lipopolysaccharide (LPS) plus anti-Ig antibodies (anti-Ig). In this assay, T cells were not detected by fluorescence-activated cell sorter analysis. It was found that both IL-2 preparations led to optimal B cell proliferation compared with supernatants obtained from murine or human spleen cells or murine cloned T helper cells. Second, we observed that the IL-2 requirement in this assay was about the same as in a proliferation assay using lectin-activated polyclonal murine Lyt-2-positive T cells. Third, analysis of the binding of radiolabeled immunoaffinity-purified IL-2 to B cells indicated that LPS plus anti-Ig-activated B cells expressed a mean of 3,500 IL-2 receptors per cell with an apparent dissociation constant of 150 pM. However, neither nonactivated B cells nor B cells activated by LPS alone exhibited significant specific IL-2 binding. The functional and the receptor data are consistent with the conclusion that IL-2 is a growth factor not only for T cells but also for B cells.
High piezoelectric performance of poly(lactic acid) film manufactured by solid-state extrusion (2014)

Recently, the application of uniaxially stretched poly(l-lactic acid) (PLLA) films to speakers, actuators, and pressure sensors has been attempted, taking advantage of their piezoelectric performance. However, the shear piezoelectric constant d14 of uniaxially stretched PLLA film is conventionally 6–10 pC N−1. To realize a high sensitivity of pressure sensors, compact speakers, and actuators, and a low driving voltage, further improvement of the piezoelectric performance is desired. In this study, we carried out solid-state extrusion (SSE) to stretch and orient poly(d-lactic acid) (PDLA) and verified its effects on piezoelectric performance. By SSE, we were able to improve the mechanical strength and elastic modulus of PDLA samples. Furthermore, the d14 of the samples was significantly increased to approximately 20 pC N−1.
WWOX Gene Expression Abolishes Ovarian Cancer Tumorigenicity In vivo and Decreases Attachment to Fibronectin via Integrin α3 (2009)

The WW domain-containing oxidoreductase (WWOX) gene is located at FRA16D, a common fragile site involved in human cancer. Targeted deletion of Wwox in mice causes increased spontaneous tumor incidence, confirming that WWOX is a bona fide tumor suppressor gene. We show that stable transfection of WWOX into human PEO1 ovarian cancer cells, containing homozygous WWOX deletion, abolishes in vivo tumorigenicity, but this does not correlate with alteration of in vitro growth. Rather, WWOX restoration in PEO1, or WWOX overexpression in SKOV3 ovarian cancer cells, results in reduced attachment and migration on fibronectin, an extracellular matrix component linked to peritoneal metastasis. Conversely, siRNA-mediated knockdown of endogenous WWOX in A2780 ovarian cancer cells increases adhesion to fibronectin. In addition, whereas there is no WWOX-dependent difference in cell death in adherent cells, WWOX-transfected cells in suspension culture display a proapoptotic phenotype. We further show that WWOX expression reduces membranous integrin alpha(3) protein but not integrin alpha(3) mRNA levels, and that adhesion of PEO1 cells to fibronectin is predominantly mediated through integrin alpha(3). We therefore propose that WWOX acts as an ovarian tumor suppressor by modulating the interaction between tumor cells and the extracellular matrix and by inducing apoptosis in detached cells. Consistent with this, the suppression of PEO1 tumorigenicity by WWOX can be partially overcome by implanting these tumor cells in Matrigel. These data suggest a possible role for the loss of WWOX in the peritoneal dissemination of human ovarian cancer cells.
Recombinant human bone morphogenetic protein 2B stimulates PC12 cell differentiation: potentiation and binding to type IV collagen. (1992)

Bone morphogenetic protein 2B (BMP 2B, also known as BMP 4) induces cartilage and bone morphogenesis in ectopic extraskeletal sites. BMP 2B is one of several bone morphogenetic proteins which along with activins and inhibins are members of the transforming growth factor-beta (TGF-beta) family. Both BMP 2B and activin A, but not TGF-beta 1, induce rat pheochromocytoma PC12 neuronal cell differentiation and expression of VGF, a nervous system-specific mRNA. PC12 cells exhibited approximately 2,500 receptors per cell for BMP 2B with an apparent dissociation constant of 19 pM. Extracellular matrix components, including fibronectin, laminin, and collagen type IV potentiated the activity of BMP and activin A, with the latter being the most active. Direct experiments demonstrated that radioiodinated BMP 2B bound to collagen type IV better than to either laminin or fibronectin. These data demonstrate a common neurotrophic activity of both BMP 2B and activin A, and suggest that these regulatory molecules alone and in conjunction with extracellular matrix components may play a role in both the development and repair of nervous tissue.
Isolation and Partial Characterization of SAA—An Amyloid-Related Protein from Human Serum (1976)

A 12,000 dalton serum amyloid A protein (SAA) has been isolated by chromatography on Sephadex in 10% formic acid. It is similar immunologically to the previously characterized 8500 dalton tissue amyloid A (AA) protein. The results of amino acid analyses, peptide maps, and the identity of the first 11 residues of the SAA and AA proteins support the idea that AA represents the amino terminal fragment of SAA and is derived from it by proteolysis.
Conformational thermostabilization of the β1-adrenergic receptor in a detergent-resistant form (2008)

There are approximately 350 non-odorant G protein-coupled receptors (GPCRs) encoded by the human genome, many of which are predicted to be potential therapeutic targets, but there are only two structures available to represent the whole of the family. We hypothesized that improving the detergent stability of these receptors and simultaneously locking them into one preferred conformation will greatly improve the chances of crystallization. We developed a generic strategy for the isolation of detergent-solubilized thermostable mutants of a GPCR, the beta1-adrenergic receptor. The most stable mutant receptor, betaAR-m23, contained six point mutations that led to an apparent T(m) 21 degrees C higher than the native protein, and, in the presence of bound antagonist, betaAR-m23 was as stable as bovine rhodopsin. In addition, betaAR-m23 was significantly more stable in a wide range of detergents ideal for crystallization and was preferentially in an antagonist conformation in the absence of ligand.
Renal Carcinoma in Patients Undergoing Nephrectomy: Analysis of Survival and Prognostic Factors (1992)

A series of 155 patients who underwent nephrectomy for renal carcinoma between 1965 and 1985 at Manchester Royal Infirmary were analysed for survival in relationship to presenting features, surgical staging and histopathology. Univariate and multivariate analyses were carried out. Five-year survival estimates for stage 1 disease were 81%, for stage 2 disease 65%, for stage 3 disease 39% and for stage 4 disease 6%. An erythrocyte sedimentation rate (ESR) greater than 30 mm/h was associated with worse survival and a history of hypertension was associated with better survival. Renal vein invasion alone was related to worse survival. Perinephric fat invasion was also associated with worse survival and this association in the multivariate analysis was more significant than expected, suggesting that the principles of radical surgery should be observed. The presence of granular cells as opposed to clear cells worsened survival. Patients with papillary tumours had a better survival than those with solid tumours.
Inhibition of angiogenesis by interleukin-12 is mediated by the interferon-inducible protein 10 (1996)

Interleukin 12 (IL-12), a multifunctional cytokine produced by macrophages and B-cell lines, induces interferon-gamma (IFN-gamma) production, stimulates growth of both T and natural killer cells, promotes Th1-type helper T-cell responses, and inhibits neovascularization. Because the human interferon-inducible protein 10 (IP-10) can also inhibit neovascularization, we tested whether IP-10, induced by IL-12 through the intermediate IFN-gamma, might be a mediator of IL-12 angiogenesis inhibition. We report here that murine IL-12 profoundly inhibited basic fibroblast growth factor (bFGF)-induced Matrigel neovascularization in vivo, and that this effect of IL-12 was neutralized by systemic administration of antibodies to either murine IFN-gamma or IP-10. Murine IL-12 induced murine IP-10 expression in mouse splenocytes, and human IFN-gamma induced human IP-10 expression in purified human endothelial cells, suggesting that IL-12 can induce IP-10 expression in certain cells. These results document the important role of IP-10 as a mediator of angiogenesis inhibition by IL-12, and raise the possibility that IP-10 may also contribute to the antitumor effect of IL-12.
The Th17/Treg Immune Imbalance in Ulcerative Colitis Disease in a Chinese Han Population (2016)

Th17/Treg immune imbalance might play a crucial role in the development of UC. To induce the production of Treg cells and TGF-β1, inhibit the level of Th17 and IL-17, and thus recover the Th17/Treg immune balance might imply new therapeutic targets in UC management.
Pulmonary oedema after percutaneous ASD-closure (2010)

Although surgery is the gold standard, percutaneous closure of an atrial septal defect (ASD) is gaining popularity. Nonoperative device closure is applicable only to secundum defects with appropriate anatomic characteristics. A 27-year-old man was referred for closure of a symptomatic secundum type ASD and suffered from a dramatic change in his clinical status due to pulmonary oedema immediately after ASD-closure. The preexisting hypertrophic cardiomyopathy, not known at the moment of the closure procedure, was responsible for this dramatic evolution. Successful occlusion of the left-to-right shunt (resulting in a closure of an "escape mechanism") imparted an acute volume loading to the patient's poorly compliant left ventricle resulting in pulmonary oedema due to hyperacute diastolic heart failure. This case report tries to highlight the haemodynamic features related to ASD closure in the presence of hypertrophic cardiomyopathy. Implications for closure procedures in the presence of poorly compliant left ventricles are discussed.
A simple colorimetric method for the determination of sugars in fruit and vegetables (1980)

Abstract A rapid, manual colorimetric method for determining glucose, fructose and sucrose in fruit and vegetables is described. The method is based on the determination of reducing sugars before and after invertase digestion using p ‐hydroxybenzoic acid hydrazide (PAHBAH). Total fructose (fructose plus fructose in sucrose) is determined using 2‐thiobarbituric acid and the component sugars calculated. Addition of sugars to fruit extracts gave good recoveries (97.4–101.8%) for glucose, fructose and sucrose.
Modeling Valuations for EuroQol Health States (1997)

The model presented in this article appears to predict the values of the states for which there are direct observations and, thus, can be used to interpolate values for the states for which no direct observations exist.
Fast-Fourier-Transform Method for Calculation of SAR Distributions in Finely Discretized Inhomogeneous Models of Biological Bodies (1984)

The paper describes a novel iterative approach for calculations of specific absorption rate (SAR) distributions in arbitrary, lossy, dielectric bodies. To date, the method has been used for 2-D problems where its accuracy has been confirmed by comparison with the analytic solutions for homogeneous and layered, circular, cylindrical bodies. With computation times that are proportional to N log/sub 2/N rather than N/sup 2/ to N/sup 3/ for the method of moments, the present approach should be extendable to 3-D bodies with N= 10/sup 4/to 10/sup 5/ cells allowing, thereby, details of SAR distributions that are needed for EM hyperthermia, as well as for assessing biological effects.
Cytoplasmic Dynein Heavy Chain 1b Is Required for Flagellar Assembly inChlamydomonas (1999)

A second cytoplasmic dynein heavy chain (cDhc) has recently been identified in several organisms, and its expression pattern is consistent with a possible role in axoneme assembly. We have used a genetic approach to ask whether cDhc1b is involved in flagellar assembly in Chlamydomonas. Using a modified PCR protocol, we recovered two cDhc sequences distinct from the axonemal Dhc sequences identified previously. cDhc1a is closely related to the major cytoplasmic Dhc, whereas cDhc1b is closely related to the minor cDhc isoform identified in sea urchins, Caenorhabditis elegans, and Tetrahymena. The Chlamydomonas cDhc1b transcript is a low-abundance mRNA whose expression is enhanced by deflagellation. To determine its role in flagellar assembly, we screened a collection of stumpy flagellar (stf) mutants generated by insertional mutagenesis and identified two strains in which portions of the cDhc1b gene have been deleted. The two mutants assemble short flagellar stumps (<1-2 micrometer) filled with aberrant microtubules, raft-like particles, and other amorphous material. The results indicate that cDhc1b is involved in the transport of components required for flagellar assembly in Chlamydomonas.
c-kit Is Expressed in Soft Tissue Sarcoma of Neuroectodermic Origin and Its Ligand Prevents Apoptosis of Neoplastic Cells (1998)

During development, mice with mutations of stem cell factor (SCF) or its receptor c-kit exhibit defects in melanogenesis, as well as hematopoiesis and gonadogenesis. Consequently, accumulating evidence suggests that the c-kit/SCF system plays a crucial role in all of these processes and in tumors which derive from them. Especially in neuroblastoma (infant tumors of neuroectoderm crest derivation such as melanocytes) it would appear that an autocrine loop exists between c-kit and SCF, and that the functional block of the c-kit receptors with monoclonal antibodies (MoAbs) results in a significant decrease in cellular proliferation. We studied the expression and role of c-kit and SCF in cell lines of soft tissue sarcoma of neuroectodermic origin, such as Ewing's sarcoma (ES) and peripheral neuro-ectodermal tumors (PNET). Using flow cytometry with MoAb CD117 PE, c-kit expression was highlighted in all six of the cell lines examined. This receptor was specifically and functionally activated by SCF, as shown by the binding experiments and the intracellular phosphotyrosine and immunoprecipitation studies that were performed. Using reverse transcriptase polymerase chain reaction analysis, five of the six cellular lines expressed the mRNA of SCF. In the medium measured by using an enzyme- linked immunosorbent assay, low concentrations of SCF were found: only the TC32 cellular line produced significantly higher levels (32 pg) than control. In serum-free culture the addition of SCF reduced the percentage of apoptotic cells from 25% to 90% in five out of the six cellular lines. This observation was confirmed by (1) the functional block of c-kit with MoAb: after 7 days of culture more than 30% of the cells were apoptotic (range 31.5% to 100%) in five out of six cell lines and there was also a decrease in the percentage of cells in phase S, and (2) c-kit antisense oligonucleotides: in the cellular lines treated with oligonucleotides (in relation to the untreated lines) there was a notable reduction (P < .001) both in the absolute number of cells and the 3H-thymidine uptake. These results indicate that ES and PNET express c-kit and its ligand SCF and that SCF is capable of protecting the tumor cells against apoptosis. Furthermore, the reverse transcriptase-polymerase chain reaction performed on the biopsies revealed the presence of mRNA both of SCF and c-kit in practically all of the samples studied. Our in vitro data lead us to assume that SCF may also inhibit tumor cell apoptosis in vivo.
ERSP: a software platform and architecture for the service robotics industry (2005)

In this paper we describe the need for, and the characteristics of a software architecture for commercial robotic products. We describe the Evolution Robotics Software Platform (ERSP/spl trade/), which provides a commercial-grade software architecture for mobile robots. The architecture has been designed to be modular, scalable, lightweight, portable, and reusable. It follows a hybrid model of data flow, combining behavior-based processing modules for real-time reactions with event-based task planning routines. We provide a detailed description of the main architectural components and some basic usage studies. We also highlight areas where compromises have been made and for which continuing work is needed.
Phospholipid binding properties of human placental anticoagulant protein-I, a member of the lipocortin family (1989)

Human placental anticoagulant protein-I (PAP-I) is a member of the lipocortin/calpactin/annexin family of Ca2+-dependent phospholipid binding proteins. PAP-I was labeled with fluorescein 5-isothiocyanate (1 mol/mol); this derivative had anticoagulant activity identical to the unlabeled protein and could be used to measure Ca2+-dependent binding to phospholipid vesicles through changes in fluorescence quenching. At 1.2 mM Ca2+, 0.50 M ionic strength, pH 7.4, 25 degrees C, fluorescein-labeled PAP-I bound to phospholipid vesicles containing 80% phosphatidylcholine, 20% phosphatidylserine with a Kd of 1.2 +/- 0.2 nM (mean +/- S.D.). At an ionic strength of 0.15 M, the Kd decreased to less than 0.1 nM. Prothrombin and factor Xa both competed with fluorescein-labeled PAP-I for binding to anionic phospholipid vesicles, but with affinities at least 1000-fold weaker than PAP-I. PAP-I bound only weakly (Kd greater than 2 x 10(-5) M) to neutral or anionic phospholipid monomers, and this binding was not calcium-dependent. These results show that the affinity of PAP-I for anionic phospholipid surfaces is sufficient to explain its potency as an in vitro anticoagulant.