gebhart/patrepeval huggingface dataset:
Quadriceps contracture due to repeated intramuscular injections - Report of operative treatment. (1987)

Forty-one patients with quadriceps contracture involving 49 limbs due to repeated intramuscular injections were treated between 1976 and 1985. Adequate follow-up studies were obtained from 37 patients (45 limbs). The operations were performed following the muscle belly approach which includes transverse division of rectus and scarred portion of vasti. The result was generally excellent. In all cases, the angle of knee flexion on prone position became better than 60° and all cases except 2 gained better than 130° of knee flexion in supine position. To prevent large dimple of thigh, we divided rectus in two steps at some distance.
Inflammatory Markers and Risk of Heart Failure in Elderly Subjects Without Prior Myocardial Infarction (2003)

In our elderly, community-based sample, a single determination of serum inflammatory markers, particularly elevated IL-6, was associated with increased risk of CHF in people without prior myocardial infarction. Additional epidemiological investigations are warranted to confirm the contribution of inflammation to the pathogenesis of CHF in the general population.
Engineering-enhanced Protein Secretory Expression in Yeast with Application to Insulin (2002)

Adaptation to efficient heterologous expression is a prerequisite for recombinant proteins to fulfill their clinical and biotechnological potential. We describe a rational strategy to optimize the secretion efficiency in yeast of an insulin precursor by structure-based engineering of the folding stability. The yield of a fast-acting insulin analogue (Asp(B28)) expressed in yeast was enhanced 5-fold by engineering a specific interaction between an aromatic amino acid in the connecting peptide and a phenol binding site in the hydrophobic core of the molecule. This insulin precursor is characterized by significantly enhanced folding stability. The improved folding properties enhanced the secretion efficiency of the insulin precursor from 10 to 50%. The precursor remains fully in vitro convertible to mature fast-acting insulin.
Amphipathic Peptide-Based Fusion Peptides and Immunoconjugates for the Targeted Ablation of Prostate Cancer Cells (2007)

We describe the design, generation, and in vitro evaluation of targeted amphipathic fusion peptides and immunoconjugates for the ablation of prostate cancer cells. The overexpression of the prostate-specific membrane antigen (PSMA) was exploited as means to specifically deliver cytotoxic peptides to prostate cancer cells. Cationic amphipathic lytic peptides were chosen as cytotoxic agents due to their ability to depolarize mitochondrial membranes and induce apoptosis. Specific delivery of the lytic peptide was facilitated by PSMA-targeting peptides and antibodies. Our results indicate that although the use of PSMA-targeted peptides only modestly enhanced the cytotoxic activity of the lytic peptide, peptide-antibody conjugates were two orders of magnitude more potent than untargeted peptide. In addition to quantifying the cytotoxic activities of the individual constructs, we also investigated the mechanisms of cell death induced by the fusion peptides and immunoconjugates. Although fusion peptides induced oncotic/necrotic death in cells, treatment with immunoconjugates resulted in apoptotic death. In summary, immunoconjugates based on lytic peptides are a promising class of therapeutics for prostate cancer therapy and warrant further investigation.
“深”观中国杯——六届中国杯帆船赛 (2012)

本次中国杯吸引了33个国家和地区的99支船队报名参赛，赛事规模创历史之最；徐莉佳助阵的华帝聚能队不负重望，以5场第一、2场第二的绝对优势获得了博纳多40．7组总冠军；中国杯不仅带动了深圳商业发展，也让帆船世界商机无限。
Bioinformatic Clonality Analysis of Next-Generation Sequencing-Derived Viral Vector Integration Sites (2012)

Clonality analysis of viral vector-transduced cell populations represents a convincing approach to dissect the physiology of tissue and organ regeneration, to monitor the fate of individual gene-corrected cells in vivo, and to assess vector biosafety. With the decoding of mammalian genomes and the introduction of next-generation sequencing technologies, the demand for automated bioinformatic analysis tools that can rapidly process and annotate vector integration sites is rising. Here, we provide a publicly accessible, graphical user interface-guided automated bioinformatic high-throughput integration site analysis pipeline. Its performance and key features are illustrated on pyrosequenced linear amplification-mediated PCR products derived from one patient previously enrolled in the first lentiviral vector clinical gene therapy study. Analysis includes trimming of vector genome junctions, alignment of genomic sequence fragments to the host genome for the identification of integration sites, and the annotation of nearby genomic elements. Most importantly, clinically relevant features comprise the determination of identical integration sites with respect to different time points or cell lineages, as well as the retrieval of the most prominent cell clones and common integration sites. The resulting output is summarized in tables within a convenient spreadsheet and can be further processed by researchers without profound bioinformatic knowledge.
The Upregulation of PI3K/Akt and MAP Kinase Pathways is Associated with Resistance of Microtubule‐Targeting Drugs in Prostate Cancer (2015)

Resistance is a significant limitation to the effectiveness of cancer therapies. The PI3K/Akt and MAP kinase pathways play important roles in a variety of normal cellular processes and tumorigenesis. This study is designed to explore the relationship of these signaling pathways with multidrug resistance in prostate cancer (PCa). The PI3K/Akt and MAP kinase pathways were investigated utilizing paclitaxel resistant DU145-TxR PCa cells and their parental non-resistant DU145 cells to determine their relationship with resistance to paclitaxel and other anticancer drugs. Our results demonstrate that the PI3K/Akt and MAP kinase pathways are upregulated in DU145-TxR cells compared to the DU145 cells. Inactivating these pathways using the PI3K/Akt pathway inhibitor LY294002 or the MAP kinase pathway inhibitor PD98059 renders the DU145-TxR cells more sensitive to paclitaxel. We investigated the effects of these inhibitors on other anticancer drugs including docetaxel, vinblastine, doxorubicin, 10-Hydroxycamptothecin (10-HCPT) and cisplatin and find that both inhibitors induces DU145-TxR cells to be more sensitive only to the microtubule-targeting drugs (paclitaxel, docetaxel and vinblastine). Furthermore, the treatment with these inhibitors induces cleaved-PARP production in DU145-TxR cells, suggesting that apoptosis induction might be one of the mechanisms for the reversal of drug resistance. In conclusion, the PI3K/Akt and MAP kinase pathways are associated with resistance to multiple chemotherapeutic drugs. Inactivating these pathways renders these PCa cells more sensitive to microtubule-targeting drugs such as paclitaxel, docetaxel and vinblastine. Combination therapies with novel inhibitors of these two signaling pathways potentially represents a more effective treatment for drug resistant PCa.
Enhanced expression of intracellular adhesion molecule-1 and P-selectin in the diabetic human retina and choroid. (1995)

Elevated expression of intercellular adhesion molecule-1 (ICAM-1) as well as E- and P-selectin occurs on the vascular endothelium in a number of disease states and is thought to play an early critical role in the adhesion of circulating leukocytes to the endothelium. The goal of the present study was to investigate the immunolocalization of these molecules in the retina and choroid of postmortem human tissue sections from nondiabetic and diabetic subjects. Whereas ICAM-1 was localized primarily within the choriocapillaris of nondiabetic subjects, immunoreactivity in diabetics was significantly elevated throughout the choroidal vasculature and within retinal blood vessels (P < 0.05). In the choroid, P-selectin was most prominent in veins of the nondiabetic, whereas in diabetics, P-selectin was significantly elevated in arteries (P < 0.001) and veins (P < 0.05) and, in some cases, was also observed in choriocapillaris. P-selectin immunoreactivity was not observed in the retina of any subject. E-selectin immunoreactivity was not observed in choroid or retina in any subjects. Neutrophil numbers per square millimeter of tissue were significantly elevated in diabetic choroid (P < 0.05) and retina (P < 0.001). Our results demonstrate that ICAM-1 and P-selectin are constitutively expressed in the normal choroid and are upregulated in the choroidal vasculature in diabetes, but only ICAM-1 was upregulated in the retina of diabetic subjects. Increased cell adhesion molecule expression may contribute to the retinal and choroidal microangiopathy observed in diabetics by enhancing leukocyte adhesion and consequently the incidence of capillary obstruction and endothelial cell injury.
The angiopathy of subarachnoid hemorrhage: angiographic and morphologic correlates. (1983)

In patients with subarachnoid hemorrhage, particularly hemorrhage due to aneurysmal rupture, there was a positive significant relation between angiographic vessel constriction and vessel pathology (angiopathy). Furthermore, there was a positive relationship between post-hemorrhage survival time and the severity of angiopathy. Factors such as age, sex, operations, steroid and CSF pressure seemed to have little affect on angiopathy following hemorrhage. Pathological changes were primarily limited to the involved major cerebral vessels themselves, with their branches rarely being affected. While intramural vascular hemorrhage was a common pathological feature in vessels showing severe pathology, the mere presence of blood surrounding an artery seemed to have little influence on vessel alterations.
Androgen receptor-mTOR crosstalk is regulated by testosterone availability: implication for prostate cancer cell survival. (2010)

The compensatory increase of AR function due to a repressed mTOR signal is advantageous for survival. Disrupting this loop at the time of initiation of androgen deprivation therapy may delay, or even prevent, the recurrence of prostate cancer.
Functional Properties of a Phenylalanine Ammonia-Lyase Promoter from Arabidopsis (1990)

Stephan Ohl, Susan A. Hedrick, Joanne Chory, Christopher J. Lamb, Functional Properties of a Phenylalanine Ammonia-Lyase Promoter from Arabidopsis, The Plant Cell, Vol. 2, No. 9 (Sep., 1990), pp. 837-848
Color from hierarchy: Diverse optical properties of micron-sized spherical colloidal assemblies (2015)

Materials in nature are characterized by structural order over multiple length scales have evolved for maximum performance and multifunctionality, and are often produced by self-assembly processes. A striking example of this design principle is structural coloration, where interference, diffraction, and absorption effects result in vivid colors. Mimicking this emergence of complex effects from simple building blocks is a key challenge for man-made materials. Here, we show that a simple confined self-assembly process leads to a complex hierarchical geometry that displays a variety of optical effects. Colloidal crystallization in an emulsion droplet creates micron-sized superstructures, termed photonic balls. The curvature imposed by the emulsion droplet leads to frustrated crystallization. We observe spherical colloidal crystals with ordered, crystalline layers and a disordered core. This geometry produces multiple optical effects. The ordered layers give rise to structural color from Bragg diffraction with limited angular dependence and unusual transmission due to the curved nature of the individual crystals. The disordered core contributes nonresonant scattering that induces a macroscopically whitish appearance, which we mitigate by incorporating absorbing gold nanoparticles that suppress scattering and macroscopically purify the color. With increasing size of the constituent colloidal particles, grating diffraction effects dominate, which result from order along the crystal's curved surface and induce a vivid polychromatic appearance. The control of multiple optical effects induced by the hierarchical morphology in photonic balls paves the way to use them as building blocks for complex optical assemblies--potentially as more efficient mimics of structural color as it occurs in nature.
ICICLES: Self-Tuning Samples for Approximate Query Answering (2000)

Approximate query answering systems provide very fast alternatives to OLAP systems when applications are tolerant to small errors in query answers. Current sampling-based approaches to approximately answer aggregate queries over foreign key joins suffer from the following drawback. All tuples in relations are deemed equally important for answering queries even though, in reality, OLAP queries exhibit locality in their data access. Consequently, they may waste precious real estate by sampling tuples that are not required at all or required very rarely. In this paper, we introduce icicles, a new class of samples that tune themselves to a dynamic workload. Intuitively, the probability of a tuple being present in an icicle is proportional to its importance for answering queries in the workload. Therefore, an icicle consists of more tuples from a subset of the relation that is required to answer more queries in the workload. Consequently, the accuracy of approximate answers obtained by using icicles is better than a static uniform random sample. We show, analytically, that for a certain class of queries reflected by the workload, icicles yield more accurate answers. In a detailed experimental study, we examine the validity and performance of icicles.
Detection of Rare Mutant Alleles by Restriction Endonuclease-mediated Selective-PCR: Assay Design and Optimization (2000)

Implementation of the assays described may facilitate development of REMS-PCR assays targeted to other loci associated with disease.
New drugs in prostate cancer (2006)

The growing field of targeted molecular therapy of prostate cancer has opened up numerous opportunities for therapeutic impact. Knowledge of the molecular determinants of progression, relapse after local therapy, chemotherapeutic resistance, and hormone refractoriness remains essential in the rational design of clinical trials of these agents. Given the complexity, heterogeneity, and crosstalk of molecular pathways and the molecular lesions in prostate cancer, combination or sequential therapy may be a necessary step towards significant therapeutic progress. Novel translational clinical trial methodologies may assist in a more rapid identification of active compounds at biologically active doses for phase-III testing.
The Particle Problem in the General Theory of Relativity (1935)

The writers investigate the possibility of an atomistic theory of matter and electricity which, while excluding singularities of the field, makes use of no other variables than the ${g}_{\ensuremath{\mu}\ensuremath{\nu}}$ of the general relativity theory and the ${\ensuremath{\phi}}_{\ensuremath{\mu}}$ of the Maxwell theory. By the consideration of a simple example they are led to modify slightly the gravitational equations which then admit regular solutions for the static spherically symmetric case. These solutions involve the mathematical representation of physical space by a space of two identical sheets, a particle being represented by a "bridge" connecting these sheets. One is able to understand why no neutral particles of negative mass are to be found. The combined system of gravitational and electromagnetic equations are treated similarly and lead to a similar interpretation. The most natural elementary charged particle is found to be one of zero mass. The many-particle system is expected to be represented by a regular solution of the field equations corresponding to a space of two identical sheets joined by many bridges. In this case, because of the absence of singularities, the field equations determine both the field and the motion of the particles. The many-particle problem, which would decide the value of the theory, has not yet been treated.
Safety and immunogenicity of the RTS,S/AS02A candidate malaria vaccine in children aged 1–4 in Mozambique (2006)

The RTS,S/AS02A was found to be safe and well tolerated. Serious adverse events were balanced between both groups and none was related to vaccination. The frequency of adverse events reported with RTS, S/AS02A was comparable to previous studies in children. Grade 3 AEs were infrequent (one case of pain, one of fever in each group and some swelling greater than 20 mm in diameter), transient and resolved without sequelae. RTS,S/AS02A was highly immunogenic for anti-circumsporozoite protein antibody response and induced a strong anti-hepatitis-B surface antigen response.
Cartilage Substitutes: Overview of Basic Science and Treatment Options (2001)

Articular cartilage defects that are symptomatic and refractory to nonoperative treatment represent a clinical management challenge. Although there have been important advances in stimulating intrinsic repair mechanisms, cartilage regeneration, and other substitution techniques, to date none has unlocked the understanding necessary to duplicate normal articular cartilage. The objectives of treatment of cartilage lesions are to obtain pain relief, reduce effusions and inflammation, restore function, reduce disability, and postpone or alleviate the need for prosthetic replacement. As the field of articular cartilage repair continues to evolve rapidly, the most appropriate treatment option for an individual patient should be based on the pathologic characteristics of the lesion and the patient's symptoms and expectations. The orthopaedic surgeon needs to be familiar with both the existing and the newly emerging cartilage treatment techniques in order to best educate patients and meet their expectations for long-term benefits.
Evidence that a slowly cycling subpopulation of adult murine epidermal cells retains carcinogen. (1986)

The distribution and persistence of radioactively labeled benzo(a)pyrene [B(a)P] in the skin of adult female SENCAR mice were investigated by autoradiography of epidermal whole mounts and cross-sections at intervals following a single initiating application of 200 nmol of either [3H]B(a)P (2 mCi) or [14C]B(a)P (23 muCi). One day after treatment, the entire thickness of the skin was labeled; the grain density was greatest over hair follicles, sebaceous glands, and interfollicular epidermis. At 1 and 2 weeks, decreases in the nuclear grain density were consistent with the overall pattern of epidermal renewal. One month after treatment, carcinogen label-retaining cells made up approximately 2% of the interfollicular basal cells. They were also present in the hair follicles, approximately 4 and 5% of basal cells in the infundibulum and external root sheath, respectively. They were rare in the germ region and dermal papilla. Carcinogen label-retaining cells were compared with slowly cycling [3H]thymidine label-retaining cells and "maturing" basal cells, two distinct proliferative subsets of adult murine epidermis. Carcinogen label-retaining cells were found to have characteristics of the slowly cycling cells: (a) most of the carcinogen labeled nuclei were found in the central regions of the epidermal proliferative units; (b) treatment of the carcinogen label-retaining cells with 2 micrograms of 12-O-tetradecanoylphorbol-13-acetate elicited labeled mitoses within 1 day, and a general decrease in grain density over basal nuclei. In contrast, maturing basal cells 4 days after a single injection of [3H]thymidine were found at the periphery of the epidermal proliferative units. Within 1 day after treatment with 2 micrograms of 12-O-tetradecanoylphorbol-13-acetate, maturing basal cells were displaced to the suprabasal layers. Double isotope-double emulsion autoradiographs demonstrated doubly labeled cells 1 month after continuous labeling with [3H]thymidine and [14C]B(a)P and provide evidence that the radioactive carcinogen is retained by the slowly cycling [3H]thymidine label-retaining cells. These observations suggest that a slowly cycling population of epidermal cells may be relevant to the initiation phase of two-stage carcinogenesis.
An immune system for cyberspace (2002)

There is legitimate concern that, within the next few years, the Internet will provide a fertile medium for new breeds of computer viruses capable of spreading orders of magnitude faster than they do today. To counter this threat, we have developed an immune system for computers that senses the presence of a previously unknown, pathogen, and then within minutes automatically derives and deploys a prescription for detecting and removing it. The system is being integrated with a commercial anti-virus product, IBM AntiVirus, and is expected to be offered as a pilot in late 1997.
Churg-Strauss syndrome: update on recent developments (2002)

Churg-Strauss syndrome (CSS) is a form of primary vasculitis characterized by allergy and angiitis. In the organ systems involved (lung, heart, peripheral nervous system, and so forth), eosinophilic infiltration can be found. Eosinophilia and normochromic normocytic anemia are leading laboratory findings together with elevated IgE. New seromarkers for the activation of endothelial cells, lymphocytes, and eosinophils (soluble thrombomodulin, soluble interleukin-2 receptor, eosinophil cationic protein) may be able to predict a relapse. Antineutrophil cytoplasmic antibodies are found in only approximately 50% of all patients with CSS, and their diagnostic value is questionable. Etiologically, hyperresponsiveness to an antigenic stimulus seems to underlie the syndrome. In asthmatics, cysteinyl leukotriene receptor type 1 antagonists are reported to trigger the disease. Cytokine profile findings on the cells involved in CSS remain contradictory. Some think CSS may be a Th2-mediated disease; its pathophysiology is not known fully. Interleukin-5 and tumor necrosis factor-alpha are elevated in serum and fluid of bronchoalveolar lavage, suggesting target cytokines for future treatment protocols. Treatment consists of glucocorticoid monotherapy. Data on outcome and effectiveness is lacking for other immunosuppressive regimens, such as cyclophosphamide or glucocorticoid plus cyclophosphamide. Treatment with interferon-alpha has been effective in patients refractory to glucocorticoid plus cyclophosphamide.