gebhart/patrepeval huggingface dataset:
Inhibition of reovirus type 3 binding to host cells by sialylated glycoproteins is mediated through the viral attachment protein (1987)

The interaction of mammalian reoviruses with sialylated glycoproteins was studied and found to be highly serotype specific in that attachment of type 3 Dearing reovirus to murine L cell receptors could be strongly inhibited by bovine submaxillary mucin (BSM), fetuin, and alpha 1 acid glycoprotein, albeit at different efficiencies, whereas attachment of type 1 Lang reovirus was inhibited only by fetuin. We subsequently demonstrated, by using reassortants between type 3 and 1 reoviruses, that inhibition of reovirus attachment to cell receptors was specified by the viral attachment protein gene S1. Using a solid-phase binding assay, we further demonstrated that the ability of reovirus type 3 or reassortant 1HA3 and the inability of reovirus type 1 or reassortant 3HA1 to bind avidly to BSM was a property of the viral S1 genome segment and required the presence of sialic acid residues on BSM oligosaccharides. Taken together, these results demonstrated that there is a serotype-specific difference in the ability of the reovirus attachment protein, sigma 1, to interact with sialylated oligosaccharides of glycoproteins. Interaction of reovirus type 3 with sialylated oligosaccharides of BSM is dramatically affected by the degree of O-acetylation of their sialic acid residues, as indicated by the findings that chemical removal of O-acetyl groups stimulated reovirus type 3 attachment to BSM, whereas preferential removal of residues lacking or possessing reduced amounts of O-acetyl groups per sialic acid molecule with Vibrio cholerae sialidase abolished binding. We also demonstrated that BSM was 10 times more potent in inhibiting attachment of infectious reovirus to L cells than was V. cholerae-treated BSM. The results are consistent with the hypothesis that sialylated oligosaccharides on host cells or erythrocytes may act as binding sites or components of binding sites for type 3 reovirus through a specific interaction with the virus attachment protein.
Co‐infection ratios versus inflammation, growth factors and progression of early atheromas (2006)

Mycoplasma pneumoniae (MP) and Chlamydophila pneumoniae (CP) antigens are encountered in complicated atheromas and may be implicated in the diversity of atherosclerotic lesions. Mycoplasma can downregulate the immune system, altering levels of inflammation, which may favor the proliferation of other co-infectious agents. In the present study we analyze whether initially stable human atheromas exhibit different ratios of MP/CP antigens compared to ongoing atheromatous lesions. Two groups were examined for the presence of inflammatory cells, macrophages, growth factors and infectious agents: Group I (GI), n=16, early stable atheromas, <4 CD68(+) macrophages/400 x field, showing a normal distribution and a fibrous cap; Group II (GII), n=14, growing atheromas, > or =4 CD68+ cells/400 x field, lacking a fibrous cap, showing a non-normal macrophage distribution. The amounts of CP (but not MP) antigens and lymphocytes in GI were significantly lower than in GII. MP/CP ratios were higher in GI. MP correlated with CP and PDGFB in GI (r=0.79 and r=0.83, p<0.001), but not in GII (r=-0.4 and r=-0.08, p=0.81). MP and CP antigens are already present in early atheromas, and a higher MP/CP ratio correlates with increased growth factors, lower inflammation and plaque stability.
A new model of visceral pain and referred hyperalgesia in the mouse (2001)

The generation of transgenic mice that lack or overexpress genes relevant to pain is becoming increasing common. However, only one visceral pain model, the writhing test, is widely used in mice. Here we describe a novel model, chemical stimulation of the colon, which we have developed in mice. Mice of either sex were injected i.v. with 30 mg/kg Evan's Blue for subsequent determination of plasma extravasation. For behavioural testing, they were placed on a raised grid and 50 microl of saline, mustard oil (0.25-2.5%) or capsaicin (0.03-0.3%) was administered by inserting a fine cannula into the colon via the anus. Visceral pain-related behaviours (licking abdomen, stretching, contractions of abdomen etc) were counted for 20 min. Before intracolonic administration, and 20 min after, the frequency of withdrawal responses to the application of von Frey probes to the abdomen was tested. The colon was removed post-mortem and the Evan's Blue content measured. Mustard oil and capsaicin administration evoked dose-dependent visceral pain behaviours, referred hyperalgesia (significant increase in responses to von Frey hairs) and colon plasma extravasation. The peak behavioural responses were evoked by 0.1% capsaicin and by 1% mustard oil respectively. The nociceptive behavioural responses were dose-dependently reversed by morphine (ED50 = 1.9 +/- 1 mg/kg s.c.). We conclude that this model represents a useful tool both for phenotyping mutant mice and for classical pharmacology since information on visceral pain, referred hyperalgesia and colon inflammation can all obtained from the same animal.
Wavelet-based nonlinear AGC method for hearing aid loudness compensation (2000)

Hearing impairment can often be corrected by medical or surgical treatment, provided the loss is not due to cochlear pathology (sensorineural loss). For sensorineural loss, the only corrective action is to wear a hearing aid. Many perceptual differences between normal and sensorineural hearing-impaired listeners are due to differences in the dB levels at which sound is detected by the ear (auditory threshold) and the associated dynamic range over which it is comfortable to listen to (loudness sensation). To adequately compensate for this, the processing of the auditory signal should be nonlinear and time-varying. Two wavelet-based compression algorithms have been developed: automatic gain control (AGC) with linear amplification and nonlinear compression AGC. The nonlinear AGC is a compression algorithm, which models loudness sensation. The wavelet transform separates the input into seven frequency bands corresponding to the critical bands of the human auditory system. For each frequency band, multiplying the wavelet coefficients by the gain can amplify or compress nonlinearly and smoothly, depending on the signal level, time and spectrum. Results suggest that the nonlinear approach, while maintaining the general spectral structure of the signal, is perceptually superior to linear AGC and compensates better for audibility loss.
Targeting ischemic brain injury with intravenous immunoglobulin (2007)

Brain ischemia induces an inflammatory response that contributes to neuronal cell death. Because of its ability to block multiple molecular events, IVIG may have particularly strong neuroprotective action against ischemic brain injury. In light of the extensive clinical experience with IVIG for other indications, development of clinical trials to evaluate the use of IVIG in human stroke patients are warranted.
Generalized Support Vector Machines (2000)

± 6 z 6 q u u¤ q q z ¤ 6 q p z u x x s Y « m u m
Infective substructures of measles virus from acutely and persistently infected cells (1979)

Ribonucleoprotein from cells acutely or persistently infected with measles virus were shown to be infectious by the calcium phosphate technique. Very little or no infectivity was obtained when calcium phosphate precipitation was omitted. Electron microscopy showed that the majority of ribonucleoprotein structures isolated from acutely infected cells were folded, whereas those from persistently infected cells were linear in appearance.
Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3 (2013)

Ubiquitination by HECT E3 enzymes regulates myriad processes, including tumor suppression, transcription, protein trafficking, and degradation. HECT E3s use a two-step mechanism to ligate ubiquitin to target proteins. The first step is guided by interactions between the catalytic HECT domain and the E2∼ubiquitin intermediate, which promote formation of a transient, thioester-bonded HECT∼ubiquitin intermediate. Here we report that the second step of ligation is mediated by a distinct catalytic architecture established by both the HECT E3 and its covalently linked ubiquitin. The structure of a chemically trapped proxy for an E3∼ubiquitin-substrate intermediate reveals three-way interactions between ubiquitin and the bilobal HECT domain orienting the E3∼ubiquitin thioester bond for ligation, and restricting the location of the substrate-binding domain to prioritize target lysines for ubiquitination. The data allow visualization of an E2-to-E3-to-substrate ubiquitin transfer cascade, and show how HECT-specific ubiquitin interactions driving multiple reactions are repurposed by a major E3 conformational change to promote ligation. DOI:http://dx.doi.org/10.7554/eLife.00828.001.
Carbohydrate-Based Vaccines against HIV/AIDS (2006)

The human immunodeficiency virus (HIV) is the cause of AIDS. Despite tremendous efforts in the past two decades, an effective HIV/AIDS vaccine capable of inducing broadly neutralizing antibody response in humans is still not on the horizon. HIV has evolved a number of mechanisms to escape the host immune surveillance, which account for the difficulties in HIV vaccine development. The outer envelope glycoprotein gp120 is heavily glycosylated. The carbohydrates cover a major area of the protein surface and constitute a strong defense mechanism for the virus to evade host immune attacks. Accumulating evidence has implicated that the HIV-1 carbohydrates themselves could also serve as targets for vaccines. A notable example is the broadly neutralizing antibody 2G12 that exerts its anti-HIV activity by targeting a multivalent oligomannose cluster on gp120. The present review provides an overview on the structure and function of HIV-1 carbohydrates as well as their relevance to HIV vaccine design. Recent work on the synthesis and antigenicity of HIV-1 carbohydrate epitopes and their mimics is highlighted.
Effects of platelet-derived growth factor and transforming growth factor-beta 1 on the synthesis of a large versican-like chondroitin sulfate proteoglycan by arterial smooth muscle cells. (1991)

Platelet-derived growth factor (PDGF) and transforming growth factor-beta 1 (TGF-beta 1) increase [35S]sulfate incorporation into proteoglycan (PG) by monkey arterial smooth muscle cells but have opposite effects on cell proliferation. The combination of these two growth regulatory peptides has an additive effect on PG synthesis but no effects on cell proliferation. The time course of sulfate incorporation after stimulation indicates that both growth factors cause maximal incorporation of sulfate into glycosaminoglycan chains by 12-18 h. The PG that is most affected is a large CSPG (Mr approximately 1.2 x 10(6)) which can be immunoprecipitated by an antibody against versican, a large CSPG synthesized by human skin fibroblasts. The hydrodynamic size of this molecule increases after PDGF and TGF-beta 1 stimulation, but the size of the core glycoprotein (Mr approximately 450,000) remains the same. Treatment with either growth factor leads to an increase in the amount of core glycoprotein for this PG. This increase correlates with an increase in the steady state level of mRNA identified by hybridization to a cDNA encoding versican. The two growth factors also increase the glycosaminoglycan chain length of this PG accounting for the greater hydrodynamic size of the molecule after stimulation. In contrast, PDGF and not TGF-beta 1 changes the composition of the glycosaminoglycan chains attached to this PG by doubling the ratio of chondroitin 6-sulfate to chondroitin 4-sulfate. These results indicate that although both of these growth factors increase the net synthesis of a large versican like CSPG, they differ in their effects on the structure of the glycosaminoglycan chains. These post-translational modifications may relate to the growth state of the cells.
Three-dimensional structure of an open form of the surface layer from the fish pathogen Aeromonas salmonicida (1989)

Cell-free culture supernatants of a lipopolysaccharide (LPS) O-polysaccharide-deficient, single-insertion transposon mutant of the tetragonal surface protein array (S layer)-containing fish pathogen Aeromonas salmonicida were examined by electron microscopy. Negative staining showed that the S layer was released as sheets of tetragonal material, indicating that although surface retention of assembled S layer requires the presence of wild-type LPS oligosaccharides, initial assembly of S-layer subunits into sheets does not require the presence of O-polysaccharide chains. The three-dimensional structure of the S layer was reconstructed from tilted micrographs of the released sheets. Horizontal sections through this reconstruction showed that the released sheets were composed of two identical S layers that were perfectly in register. The reconstructed layer had a lattice constant of 12.5 nm. At a resolution of 1.6 nm, the layer consisted of a major tetragon at one fourfold axis of symmetry and a minor tetragon at the second fourfold axis of symmetry. The core, composed of four of the major domains, contained a large depression and was located toward the inside of the layer. The minor tetragon provided connectivity within the layer and was located toward the outer surface of the layer. Projections through the double layer gave a type I (closed) pattern (M. Stewart, T. J. Beveridge, and T. J. Trust, J. Bacteriol. 166:120-127, 1986), yet projections through the single layer indicated that the type II (open) pattern was present. This open pattern was indistinguishable from that seen in S layer released from the surfaces of wild-type cells.
Not so easy being green (2001)

Small and medium enterprises (SMEs) believe that the environment is an important issue, and support ecological protection, but they seem to fall short in their awareness of formal environmental management systems, specific environmental laws and/or remediation processes.
DTR-GAN: Dilated Temporal Relational Adversarial Network for Video Summarization (2018)

The large amount of videos popping up every day, make it is more and more critical that key information within videos can be extracted and understood in a very short time. Video summarization, the task of finding the smallest subset of frames, which still conveys the whole story of a given video, is thus of great significance to improve efficiency of video understanding. In this paper, we propose a novel Dilated Temporal Relational Generative Adversarial Network (DTR-GAN) to achieve frame-level video summarization. Given a video, it can select a set of key frames, which contains the most meaningful and compact information. Specifically, DTR-GAN learns a dilated temporal relational generator and a discriminator with three-player loss in an adversarial manner. A new dilated temporal relation (DTR) unit is introduced for enhancing temporal representation capturing. The generator aims to select key frames by using DTR units to effectively exploit global multi-scale temporal context and to complement the commonly used Bi-LSTM. To ensure that the summaries capture enough key video representation from a global perspective rather than a trivial randomly shorten sequence, we present a discriminator that learns to enforce both the information completeness and compactness of summaries via a three-player loss. The three-player loss includes the generated summary loss, the random summary loss, and the real summary (ground-truth) loss, which play important roles for better regularizing the learned model to obtain useful summaries. Comprehensive experiments on two public datasets SumMe and TVSum show the superiority of our DTR-GAN over the state-of-the-art approaches.
A Controlled Trial of Nonoxynol 9 Film to Reduce Male-to-Female Transmission of Sexually Transmitted Diseases (1998)

The use of a nonoxynol 9 vaginal film did not reduce the rate of new HIV, gonorrhea, or chlamydia infection in this group of sex workers who used condoms and received treatment for sexually transmitted diseases.
Intermittent Positive-Pressure Ventilation, Chest Compression Synchronized Ventilation, Bilevel Ventilation, Continuous Chest Compression, Active Compression Decompression, and Impedance Threshold Device—The Complexity of Ventilation During Cardiopulmonary Resuscitation* (2014)

Services des Urgences, Hôpital Lariboisière, University Paris Diderot, Paris, France * See also p. e89. The author has disclosed that he does not have any potential conflicts of interest.
The Du Art Frame Count Cueing System (1981)

Computer technology now provides an alternative to the traditional system of notching motion picture film to insure proper handling during processing. Du Art Laboratories has developed a system which utilizes a minicomputer for processing from the negative room through timing, printing, and into print correction. The use of computer technology, coupled with a sophisticated frame counting technique, makes film timing and cueing easier and safer, reducing film handling and lessening the possibility of scratches or errors.
High‐Performance n‐ and p‐Type Single‐Crystal Organic Transistors with Free‐Space Gate Dielectrics (2004)

A novel single-crystal organic field-effect transistor (OFET) is described, in which the gate dielectric is replaced by a thin gap that can be occupied by a gas or vacuum. When used in combination with high-quality rubrene or tetracyanoquinodimethane (TCNQ) crystals, n- and p-devices with extremely good mobilities and normalized sub-threshold slopes, representing the ultimate in OFET performance, are realized. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2089/2004/c1017_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Heparin-induced thrombocytopenia (2004)

Heparin is widely used for the prevention and treatment of thrombotic and particularly cardiovascular disorders. Unfortunately, 0.5 to 3.0% of patients given heparin develop an immune reaction, commonly termed Type II heparin-induced thrombocytopenia (HIT). This is characterized by a moderate thrombocytopenia and in some patients, a venous or arterial thrombosis. This frequently leads to disastrous sequelae, such as limb amputation and death. The pathophysiological basis of this serious adverse drug reaction is the production of an immunoglobulin G antibody that reacts with an antigenic complex consisting of heparin and platelet factor 4. A significant risk factor for the development of HIT is recent surgery, and the frequency of developing an antiheparin-platelet factor 4 or HIT antibody is particularly high in cardiac surgery patients, although surprisingly, only a few of these patients actually develop the clinical syndrome of HIT. This review will discuss the frequency, pathophysiology, clinical features, diagnosis and management of HIT.
Activity Prediction: A Twitter-based Exploration (2012)

Social media platforms allow users to share their messages with everyone else. In microblogs, e.g., Twitter, people mostly report on what they did, they talk about current activities, and mention things they plan to do in the near future. In this paper, we propose the task of activity prediction, that is, trying to establish a set of activities that are likely to become popular at a later time. We perform a small-scale initial experiment, in which we try to predict popular activities for the coming evening using Dutch Twitter data. Our experiment shows the feasibility and challenges of the task, with a simple method resulting in human-readable activities. This exploration also identifies several issues (e.g., temporal phrases and activity classification) that need to be addressed in future work.
Pore-Spanning Lipid Bilayers Visualized by Scanning Force Microscopy (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTPore-Spanning Lipid Bilayers Visualized by Scanning Force MicroscopyChristian Hennesthal and Claudia SteinemView Author Information Institut für Biochemie, Westfälische Wilhelms-Universität Wilhelm-Klemm-Strasse 2, 48149 Münster, Germany Cite this: J. Am. Chem. Soc. 2000, 122, 33, 8085–8086Publication Date (Web):August 8, 2000Publication History Received16 March 2000Revised7 July 2000Published online8 August 2000Published inissue 23 August 2000https://pubs.acs.org/doi/10.1021/ja000940jhttps://doi.org/10.1021/ja000940jrapid-communicationACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views1071Altmetric-Citations77LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Gold,Layers,Membranes,Oxides,Vesicles Get e-Alerts