gebhart/patrepeval huggingface dataset:
Sweat Testing in Opioid Users with a Sweat Patch (1996)

For many years, toxicologists have detected the presence of drugs of abuse in biological materials using blood or urine. In recent years, remarkable advances in sensitive analytical techniques have enabled the analysis of drugs in unconventional samples such as sweat. In a study conducted in a detoxification center, sweat patches were applied to 20 known heroin abusers. Subjects wore the patch with minimal discomfort for five days. During the same period, two urine specimens were also collected. Target drugs analyzed either by gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-mass spectrometry (LC-MS) included opiates (heroin, 6-monoacetylmorphine, morphine, codeine), cocaine (cocaine, benzoylecgonine, ecgonine methyl ester), delta 9-tetrahydrocannabinol, benzodiazepines (nordiazepam, oxazepam), amphetamines (amphetamine, methamphetamine, methylenedioxyamphetamine [MDA], methylenedioxymethamphetamine [MDMA], methylenedioxyethylamphetamine [MDEA]), and buprenorphine. Patches were positive for opiates in 12 cases. Heroin (37-175 ng/patch) and/or 6-acetylmorphine (60-2386 ng/patch) were identified in eight cases, and codeine exposure (67-4018 ng/patch) was determined in four cases. When detected, heroin was always present in lower concentrations than 6-acetylmorphine, which was the major analyte found in sweat. Cocaine (324 ng/patch) and metabolites were found in only one case. delta 9-Tetrahydrocannabinol (4-38 ng/patch) was identified in nine cases. Benzodiazepine concentrations were very low, ranging from 2 to 44 and from 2 to 15 ng/patch for nordiazepam and oxazepam, respectively. MDEA (121 ng/patch) and its metabolite, MDA (22 ng/patch), were detected in one case. Buprenorphine, which was administered as therapy under close medical supervision, was detected in the range 1.3-153.2 ng/patch with no apparent relationship between the daily dose and amount excreted in sweat. All the urine tests were consistent with the sweat findings, but to identify the same drugs it was necessary to test two urine specimens along with only one sweat specimen. It was concluded that sweat testing appears to offer the advantage of being a relatively noninvasive means of obtaining a cumulative estimate of drug exposure over the period of a week. This new technology may find useful applications in the treatment and monitoring of substance abusers, as the patch provides a long-term continuous monitor of drug exposure or noncompliance.
Preparation and in vitro transfection efficiency of chitosan microspheres containing plasmid DNA:poly(L-lysine) complexes. (2004)

As a conclusion, pDNA:PLL complexes could be encapsulated into chitosan microspheres with maintaining their structural and functional integrity and this system may be a good alternative for polycation based gene carriers.
Liposome-incorporated ciprofloxacin in treatment of murine salmonellosis (1993)

We used a dehydration-rehydration procedure in order to efficiently incorporate ciprofloxacin into phospholipid vesicles (liposomes), which we then used to treat BALB/c mice that had been infected per os with Salmonella dublin. A single injection of liposome-incorporated ciprofloxacin (LIC) was 10 times more effective than a single injection of free drug at preventing mortality. When free ciprofloxacin was administered twice daily for 5 days, it was more effective than when it was administered as a single dose. Treatment with LIC produced dose-dependent decreases in bacterial counts in spleen, stool, and Peyer's patches, indicating that the drug had distributed to all areas of inflammation, not just to the major reticuloendothelial system organs. Although LIC was cleared rapidly from the blood, drug persisted in the liver and spleen for at least 48 h after administration of a dose of LIC. We attribute the enhanced activity of LIC to the concentration and persistence of active drug in the infected organs. Our results confirm the fact that ciprofloxacin is effective therapy for systemic salmonella infection and show that LIC is even more effective than aqueous ciprofloxacin in our model.
The SPOSAD Architectural Style for Multi-tenant Software Applications (2011)

A multi-tenant software application is a special type of highly scalable, hosted software, in which the application and its infrastructure are shared among multiple tenants to save development and maintenance costs. The limited understanding of the underlying architectural concepts still prevents many software architects from designing such a system. Existing documentation on multi-tenant software architectures is either technology-specific or database-centric. A more technology-independent perspective is required to enable wide-spread adoption of multi-tenant architectures. We propose the SPOSAD architectural style, which describes the components, connectors, and data elements of a multi-tenant architecture as well as constraints imposed on these elements. This paper describes the benefits of a such an architecture and the trade-offs for the related design decisions. To evaluate our proposal, we illustrate how concepts of the style help to make current Platform-as-a-Service (PaaS) environments, such as Force.com, Windows Azure, and Google App Engine scalable and customizable.
A Convenient Preparation of 1-Diazopropane (1964)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA Convenient Preparation of 1-DiazopropaneJohn R. Dyer, R. B. Randall Jr., and Howard M. DeutschCite this: J. Org. Chem. 1964, 29, 11, 3423–3424Publication Date (Print):November 1, 1964Publication History Published online1 May 2002Published inissue 1 November 1964https://pubs.acs.org/doi/10.1021/jo01034a517https://doi.org/10.1021/jo01034a517research-articleACS PublicationsRequest reuse permissionsArticle Views176Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Conversational Telephone Messaging System (1984)

The Phone Slave is an intelligent answering machine, conversing with callers to format messages and relaying personal greetings to identified partles. Its owner can access these voice messages as well as electronic mail via speech recognition or Touch-Tones over the phone network. Access to both lncoming and outgoing messages, an on-line directory, and autodial features are also provided by a touch-sensitive color monitor.
Excellence in Research: Creative Organizational Responses at Berkeley, Harvard, MIT, and Stanford (1985)

Research environments of four leading universities were studied with focus on organizational responses for encouraging collaboration in research there, as well as implications for encouraging excellence in research at other institutions. (ERIC ED 259 624, 41 pp, MF $.97, PC $5.34).
Recent Advances in Petroleum Microbiology (2003)

Recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. The physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. New molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystems. By establishing conditions which maximize rates and extents of microbial growth, hydrocarbon access, and transformation, highly accelerated and bioreactor-based petroleum waste degradation processes have been implemented. Biofilters capable of removing and biodegrading volatile petroleum contaminants in air streams with short substrate-microbe contact times (<60 s) are being used effectively. Microbes are being injected into partially spent petroleum reservoirs to enhance oil recovery. However, these microbial processes have not exhibited consistent and effective performance, primarily because of our inability to control conditions in the subsurface environment. Microbes may be exploited to break stable oilfield emulsions to produce pipeline quality oil. There is interest in replacing physical oil desulfurization processes with biodesulfurization methods through promotion of selective sulfur removal without degradation of associated carbon moieties. However, since microbes require an environment containing some water, a two-phase oil-water system must be established to optimize contact between the microbes and the hydrocarbon, and such an emulsion is not easily created with viscous crude oil. This challenge may be circumvented by application of the technology to more refined gasoline and diesel substrates, where aqueous-hydrocarbon emulsions are more easily generated. Molecular approaches are being used to broaden the substrate specificity and increase the rates and extents of desulfurization. Bacterial processes are being commercialized for removal of H(2)S and sulfoxides from petrochemical waste streams. Microbes also have potential for use in removal of nitrogen from crude oil leading to reduced nitric oxide emissions provided that technical problems similar to those experienced in biodesulfurization can be solved. Enzymes are being exploited to produce added-value products from petroleum substrates, and bacterial biosensors are being used to analyze petroleum-contaminated environments.
System Combination for Multi-document Summarization (2015)

We present a novel framework of system combination for multi-document summarization.
A thrombin receptor function for platelet glycoprotein Ib–IX unmasked by cleavage of glycoprotein V (2001)

Glycoprotein (GP) V is a major substrate cleaved by the protease thrombin during thrombin-induced platelet activation. Previous analysis of platelets from GP V-null mice suggested a role for GP V as a negative modulator of platelet activation by thrombin. We now report the mechanism by which thrombin activates GP V -/- platelets. We show that proteolytically inactive forms of thrombin induce robust stimulatory responses in GP V null mouse platelets, via the platelet GP Ib--IX--V complex. Because proteolytically inactive thrombin can activate wild-type mouse and human platelets after treatment with thrombin to cleave GP V, this mechanism is involved in thrombin-induced platelet aggregation. Platelet activation through GP Ib-IX depends on ADP secretion, and specific inhibitors demonstrate that the recently cloned P2Y(12) ADP receptor (G(i)-coupled ADP receptor) is involved in this pathway, and that the P2Y(1) receptor (G(q)-coupled ADP receptor) may play a less significant role. Thrombosis was generated in GP V null mice only in response to catalytically inactive thrombin, whereas thrombosis occurred in both genotypes (wild type and GP V null) in response to active thrombin. These data support a thrombin receptor function for the platelet membrane GP Ib--IX--V complex, and describe a novel thrombin signaling mechanism involving an initiating proteolytic event followed by stimulation of the GP Ib--IX via thrombin acting as a ligand, resulting in platelet activation.
K+ inward rectifier currents in reactive astrocytes from adult rat brain (1999)

We characterized inward rectifier (K(IR)) currents in reactive astrocytes activated by CNS injury. We used primary cultures of reactive astrocytes obtained from gelatin sponge implants in adult rat brains, a system that yielded highly purified, homogeneous cultures with >95% of cells positive for GFAP, vimentin, and S-100beta. Ionic channels were studied in 1-21-day-old primary cultures using a nystatin-perforated patch clamp technique. Fast Na(+) currents were identified in <2% of cells. Most cells exhibited outward currents positive to -50 mV, with one component being sensitive to charybdotoxin, iberiotoxin, and tetraethylammonium chloride, and another component being sensitive to 4-aminopyridine. Two populations of cells were distinguished, based on presence or absence of Ba(2+)-sensitive K(IR) current negative to the K(+) reversal potential (E(k)), with >80% of cells expressing K(IR) currents. In contrast to previous reports on mammalian astrocytes, the current-voltage curve showed no appreciable current between E(k) and -50 mV, reflecting strong rectification by K(IR) channels. The magnitude of K(IR) current at -130 mV (I(-)(130)) did not change significantly during 21 days in culture (123 cells), suggesting constitutive expression of K(IR) channels. The fraction of K(IR)-negative cells was not affected by serum-starvation for 16-24 h. In cells with I(-)(130) >/= -30 pA, the membrane potential was invariably near E(k) and depolarized appreciably on addition of Ba(2+), but in cells with I(-)(130) < -30 pA, resting potentials ranged from -40 mV to -90 mV. We conclude that most adult reactive astrocytes constitutively express K(IR) channel(s) that exhibit strong rectification not previously observed in mammalian astrocytes.
Investigation of Cr-doped MgO and Sc2O3 as potential laser sources for the near infrared spectral range (2000)

Cr-doped MgO and Sc 2 O 3 were spectroscopically investigated with respect to their feasibility as solid state laser materials. The crystal field parameters of the octahedrally coordinated Cr 3+ were determined to be Dq = 1615 cm -1 , B = 586 cm -1 , and C = 3249 cm -1 , thus Dq/B = 2.75 and C/B = 5.54 for Cr 3+ : MgO and Dq = 1490 cm -1 , B = 590 cm -1 , C = 3227 cm -1 , thus Dq/B = 2.53, and C/B = 5.47 for Cr 3+ : Sc 2 O 3 . The Cr 3+ emission in these crystals covers the spectral region between 700 nm and 1100 nm. Besides the Cr 3+ -ion, in Sc 2 O 3 another optical active center correlated with chromium is observed. This center exhibits absorption between 750 nm and 1100 nm and emission between 1050 nm and 1800 nm. For Cr 3+ : MgO room temperature, continuous wave laser oscillation under argon ion laser pumping was realized.
FReeNet: Multi-Identity Face Reenactment (2019)

This paper presents a novel multi-identity face reenactment framework, named FReeNet, to transfer facial expressions from an arbitrary source face to a target face with a shared model. The proposed FReeNet consists of two parts: Unified Landmark Converter (ULC) and Geometry-aware Generator (GAG). The ULC adopts an encode-decoder architecture to efficiently convert expression in a latent landmark space, which significantly narrows the gap of the face contour between source and target identities. The GAG leverages the converted landmark to reenact the photorealistic image with a reference image of the target person. Moreover, a new triplet perceptual loss is proposed to force the GAG module to learn appearance and geometry information simultaneously, which also enriches facial details of the reenacted images. Further experiments demonstrate the superiority of our approach for generating photorealistic and expression-alike faces, as well as the flexibility for transferring facial expressions between identities.
Combinatorial Synthesis of Peptide Arrays onto a Microchip (2007)

Arrays promise to advance biology through parallel screening for binding partners. We show the combinatorial in situ synthesis of 40,000 peptide spots per square centimeter on a microchip. Our variant Merrifield synthesis immobilizes activated amino acids as monomers within particles, which are successively attracted by electric fields generated on each pixel electrode of the chip. With all different amino acids addressed, particles are melted at once to initiate coupling. Repetitive coupling cycles should allow for the translation of whole proteomes into arrays of overlapping peptides that could be used for proteome research and antibody profiling.
Successful Treatment of Experimental Escherichia coli Infections in Mice Using Phage: its General Superiority over Antibiotics (1982)

Anti-K 1 phages were more active in vitro and in vivo against an 018:K1:H7 ColV+ Escherichia coli strain, designated MW, than were other phages. A single intramuscular dose of one anti-K1 phage was more effective than multiple intramuscular does of tetracycline, ampicillin, chloramphenicol, or trimethoprim plus sulphafurazole in curing mice of a potentially lethal intramuscularly or intracerebrally induced infection of MW; it was at least as effective as multiple intramuscular doses of streptomycin. When MW and the phage were inoculated into different gastrocnemius muscles of the same mice, a rapid reduction in numbers of MW organisms occurred in the MW-inoculated muscle and in other tissues; the numbers of phage particles in the MW-inoculated muscle increased rapidly and greatly. MW failed to proliferate in the brains of intracerebrally infected mice that had been inoculated intramuscularly with the phage at the same time; many more phage particles were found in the brains of these mice than in other sites. The few phage-resistant mutants of MW found in the phage-treated mich were K1-; previous studies had shown such mutants to be of greatly reduced virulence. The phage administered intramuscularly 3-5 d before challenge with a potentially lethal intramuscularly induced infection of MW was protective, the protective effect varying between phage propagated on different bacterial strains.
Full-field characterization of femtosecond pulses by spectrum and cross-correlation measurements (1999)

We present a practical and accurate technique for retrieving the amplitude and the phase of ultrashort pulses from a nonlinear (second-order) intensity cross correlation and the spectrum that overcomes shortcomings of previous attempts. We apply the algorithm to theoretical and experimental data and compare it with frequency-resolved optical gating.
Isometric Collision Rules for the Four-Dimensional FCHC Lattice Gas (1987)

Collision r ules are present ed for th e four-dimensional face­ centered-hypercubi c-lattice (FCHC). The velocity set after collision is deduced from th e velocity set be fore collision by an isometry, cho­ sen so as to pr eserve th e momentum and minimiz e t he viscosity . A detailed implementation recipe is given. Th e shea r viscosity is ca m­ puted ; th e result shows that essentiall y all memory of t he previous velocities is lost at each collision. A not her set of collision rule s, based on a r andom choice of t he output state, has similar pr ope rties . The isometri c p rin cipl e c an also be applied to t he two-dimension al squar e (HPP) an d triangular (FHP) latti ces : o ne recovers t he usual rule s with minor differ ences .
Nanowire Substrate-Based Laser Scanning Cytometry for Quantitation of Circulating Tumor Cells (2012)

We report on the development of a nanowire substrate-enabled laser scanning imaging cytometry for rare cell analysis in order to achieve quantitative, automated, and functional evaluation of circulating tumor cells. Immuno-functionalized nanowire arrays have been demonstrated as a superior material to capture rare cells from heterogeneous cell populations. The laser scanning cytometry method enables large-area, automated quantitation of captured cells and rapid evaluation of functional cellular parameters (e.g., size, shape, and signaling protein) at the single-cell level. This integrated platform was first tested for capture and quantitation of human lung carcinoma cells from a mixture of tumor cells and leukocytes. We further applied it to the analysis of rare tumor cells spiked in fresh human whole blood (several cells per mL) that emulate metastatic cancer patient blood and demonstrated the potential of this technology for analyzing circulating tumor cells in the clinical settings. Using a high-content image analysis algorithm, cellular morphometric parameters and fluorescence intensities can be rapidly quantitated in an automated, unbiased, and standardized manner. Together, this approach enables informative characterization of captured cells in situ and potentially allows for subclassification of circulating tumor cells, a key step toward the identification of true metastasis-initiating cells. Thus, this nanoenabled platform holds great potential for studying the biology of rare tumor cells and for differential diagnosis of cancer progression and metastasis.
Comparison of the DNA Extraction Methods for Polymerase Chain Reaction Amplification from Formalin-Fixed and Paraffin-Embedded Tissues (2001)

To obtain an adequate quality and quantity of DNA from formalin-fixed and paraffin-embedded tissue, six different DNA extraction methods were compared. Four methods used deparaffinization by xylene followed by proteinase K digestion and phenol-chloroform extraction. The temperature of the different steps was changed to obtain higher yields and improved quality of extracted DNA. The remaining two methods used microwave heating for deparaffinization. The best DNA extraction method consisted of deparaffinization by microwave irradiation, protein digestion with proteinase K at 48 degrees C overnight, and no further purification steps. By this method, the highest DNA yield was obtained and the amplification of a 989-base pair beta-globin gene fragment was achieved. Furthermore, DNA extracted by means of this procedure from five gastric carcinomas was successfully used for single strand conformation polymorphism and direct sequencing assays of the beta-catenin gene. Because the microwave-based DNA extraction method presented here is simple, has a lower contamination risk, and results in a higher yield of DNA compared with the ordinary organic chemical reagent-based extraction method, it is considered applicable to various clinical and basic fields.
Combined Chemical‐Enzymatic Assembly of Aminoglycoside Derivatives with N‐1‐AHB Side Chain (2008)

Abstract A series of unprotected pseudo‐disaccharides and pseudo‐trisaccharides of 2‐deoxystreptamine‐containing aminoglycosides have been selectively acylated at the N‐1 position with the valuable ( S )‐4‐amino‐2‐hydroxybutanoyl (AHB) pharmacophore by using the recombinant BtrH and BtrG enzymes from butirosin biosynthesis in combination with a synthetic acyl donor. The process was optimized by performing two enzymatic steps in a sequential manner without purification of the intermediate product.