gebhart/patrepeval huggingface dataset:
Murine mucopolysaccharidosis type VII: long term therapeutic effects of enzyme replacement and enzyme replacement followed by bone marrow transplantation. (1997)

We demonstrated previously that short term administration of recombinant beta-glucuronidase to newborn mice with mucopolysaccharidosis type VII reduced lysosomal storage in many tissues. Lysosomal storage accumulated gradually after cessation of enzyme replacement therapy. Mice alive at 1 yr of age had decreased bone deformities and less lysosomal storage in cortical neurons. Here we compare the effects of long term enzyme replacement initiated either at birth or at 6 wk of age, and of enzyme administration initiated at birth followed by syngeneic bone marrow transplantation (BMT) at 5 wk of age. Several mice from each treatment group lived to at least 1 yr of age. Liver and spleen samples had beta-glucuronidase levels ranging from 2.4 to 19.8% of normal and showed a parallel decrease in lysosomal storage. The combination of enzyme replacement therapy followed by BMT reduced lysosomal distension in meninges, corneal fibroblasts, and bone when compared with treatment with enzyme alone. Mice treated at birth had less lysosomal storage in some neurons of the brain and the skeletal dysplasia was less severe when compared to mice whose treatment was delayed until 6 wk of age. We conclude that both enzyme replacement alone and early enzyme replacement followed by BMT have long term positive effects on murine mucopolysaccharidosis type VII. In addition, treatment started at birth is far more effective than treatment initiated in young adults.
Redesigning the DNA‐binding specificity of a zinc finger protein: A data base‐guided approach (1992)

A peptide corresponding to the three zinc finger domains of the human transcription factor Sp1 has been expressed and found to bind a consensus Sp1 binding site with the sequence 5'-GGGGCGGGG-3'. Examination of the amino acid distributions within a large zinc finger sequence data base and chemical arguments suggested that a particular Arg to Gln sequence change might convert binding specificity to 5'-GGGGCAGGG-3'. Experimental tests of this hypothesis revealed that such a change could be induced only when two other sequence changes, deduced from examination of sequence correlations, were made as well. These results provide the most direct information to date about how zinc finger proteins might recognize adenine-containing binding sites and bear on the existence and nature of any code between zinc finger protein and binding site sequences.
Measuring method of three-dimensional position and orientation of a moving object for medical robots (2002)

A measuring method of 3-D position and orientation of a moving object has been developed, which employed a 2-D position measuring instrument (with only one camera) and three light points attached to the moving object. In order to increase the accuracy of the 3-D measurement, the positions of pupils of the optical system for the 2-D position measuring instrument were introduced to derive the theoretical equations, and the geometrical relation between three light points in the object space was determined experimentally using the 2-D position measuring instrument before conducting 3-D measurements. Based on the principle of our measuring method, static 3-D measurements were conducted. The results showed that the maximum measurement errors of the X, Y, and Z coordinates of three light points were 0.05 mm, 0.1 mm, and 3 mm respectively within the measuring range from Z=-360 mm to 2=-760 mm. The accuracy of the measurement will be increased to the resolution of the 2-D position measuring instrument by improving the experimental apparatus and method.
Prevention of chemotherapy-induced ulcerative mucositis by transforming growth factor beta 3. (1994)

Mucositis is a common, dose-limiting complication in patients receiving cancer chemotherapy, which appears to be a consequence of the rate of epithelial proliferation. The beta transforming growth factors have been shown to be negative regulators of epithelial cell proliferation. Here we show that transforming growth factor beta 3 administration reduced proliferation of oral epithelium in vitro and in vivo. Topical application of transforming growth factor beta 3 to the oral mucosa of the Syrian golden hamster prior to chemotherapy significantly reduced the incidence, severity, and duration of oral mucositis, reduced chemotherapy-associated weight loss, and increased survival.
”tillinni jipiri riviri killiri birg mid riksmik” : Öyvind Fahlströms översättningsstrategier (2010)

”tillinni jipiri riviri killiri birg mid riksmik” : Oyvind Fahlstroms oversattningsstrategier
Battery life estimation of mobile embedded systems (2001)

Since battery life directly impacts the extent and duration of mobility, one of the key considerations in the design of a mobile embedded system should be to maximize the energy delivered by the battery, and hence the battery lifetime. To facilitate exploration of alternative implementations for a mobile embedded system, in this paper we address the issue of developing a fast and accurate battery model, and providing a framework for battery life estimation of Hardware/Software (HW/SW) embedded systems. We introduce a stochastic model of a battery, which can simultaneously model two key phenomena affecting the battery life and the amount of energy that can be delivered by the battery: the rate capacity effect and the recovery effect. We model the battery behavior mathematically in terms of parameters that can be related to physical characteristics of the electro-chemical cell. We show how this model can be used for battery life estimation of a HW/SW embedded system, by calculating battery discharge demand waveforms using a power co-estimation technique. Based on the discharge demand, the battery model estimates the battery lifetime as well as the delivered energy. Application of the battery life estimation methodology to three system implementations of an example TCP/IP network interface subsystem demonstrates that different system architectures can have significantly different delivered energy and battery lifetimes.
Optimization of Pyrazoles as Phenol Surrogates to Yield Potent Inhibitors of Macrophage Migration Inhibitory Factor (2018)

Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that is implicated in the regulation of inflammation, cell proliferation, and neurological disorders. MIF is also an enzyme that functions as a keto-enol tautomerase. Most potent MIF tautomerase inhibitors incorporate a phenol, which hydrogen bonds to Asn97 in the active site. Starting from a 113-μm docking hit, we report results of structure-based and computer-aided design that have provided substituted pyrazoles as phenol alternatives with potencies of 60-70 nm. Crystal structures of complexes of MIF with the pyrazoles highlight the contributions of hydrogen bonding with Lys32 and Asn97, and aryl-aryl interactions with Tyr36, Tyr95, and Phe113 to the binding.
Cx31.1 acts as a tumour suppressor in non‐small cell lung cancer (NSCLC) cell lines through inhibition of cell proliferation and metastasis (2011)

Reduced connexin expression and loss of gap junction function is a characteristic of many cancers, including lung cancer. However, there are little reports about the relation between Cx31.1 and lung cancer. This study was conducted to investigate the effect of Cx31.1 on non-small cell lung cancer (NSCLC). We found that the Cx31.1 was down-regulated in NSCLC cell lines, and the expression levels were reversely related with their metastatic potential. We ectopically expressed Cx31.1 in H1299 NSCLC cell line to examine the influence of Cx31.1 overexpression. The results showed that overexpression of Cx31.1 in H1299 cells reduced cell proliferation, induced a delay in the G(1) phase, inhibited anchorage-independent growth and suppressed cell migration and invasion. The cell cycle delay and cell migration and invasion suppressive effects of Cx31.1 were partially reversed by siRNA targeting mRNA of Cx31.1. Moreover, xenografts of Cx31.1 overexpressing H1299 cells showed reduced tumourigenicity. These results suggested that Cx31.1 has tumour-suppressive properties. Further investigation indicated that cyclin D3 may be responsible for Cx31.1-induced G(1) phase delay. Importantly, Cx31.1 increased the expression of epithelial markers, such as cytokeratin 18, and decreased expression of mesenchymal markers, such as vimentin, indicating a Cx31.1-mediated partial shift from a mesenchymal towards an epithelial phenotype. We concluded that Cx31.1 inhibit the malignant properties of NSCLC cell lines, the mechanisms under this may include regulation of EMT.
4-Hydroxynonenal triggers an epidermal growth factor receptor-linked signal pathway for growth inhibition (1999)

Lipid peroxidation has been implicated in the pathogenesis of various diseases. As a major product of membrane lipid peroxidation, 4-hydroxynonenal (HNE) appears after various kinds of oxidative stress, and is known to induce cell growth inhibition. We here analysed the HNE-mediated signal transduction cascade for the growth inhibition of human epidermoid carcinoma A431 cells. HNE dose-dependently induced phosphorylation of multiple cellular proteins including epidermal growth factor receptor (EGFR) in A431 cells, and rapidly upregulated the catalytic actions of EGFR for autophosphorylation and for phosphorylation of casein as an exogenous substrate. Immunoblot analysis by use of HNE-specific antibody demonstrated the binding of HNE to EGFR along with its activation. This binding, which did not induce cross-linking of EGFR, caused a capping of the receptor on the cell surface which mimicked the capping induced by EGF. Phosphorylation and activation of EGFR were followed by phosphorylation of adaptor protein Shc and activation of MAP kinase. Both genistein as a wide spectrum protein tyrosine kinase inhibitor and AG1478 as a specific EGFR tyrosine phosphorylation blocker inhibited activation of EGFR and MAP kinase by HNE. The same inhibitors prevented HNE-mediated growth inhibition, suggesting a close linkage between EGFR/MAP kinase activation and growth inhibition after exposure to HNE. Our results suggest that EGFR may be one of the primary targets of HNE for an oxidative stress-linked cell growth inhibition.
Espiritualidade Cristã Contemporânea à Brasileira e Suas Raízes Antimaçônicas (2020)

O presente artigo buscou refletir sobre a história da antimaçonaria, presente no imaginário popular do cristianismo brasileiro. Ao longo do texto, foi possível observar que o potencial político da Maçonaria e sua filosofia progressista, foram fatores determinantes para as condenações advindas do papado romano. Do mesmo modo, este artigo explorou o pensamento de pensadores adeptos do antimaçonismo, uma vez que durante os séculos XIX e XX a Igreja Católica Romana patrocinou alguns desses autores. Por fim, foi possível observar que após a descoberta de diversas fraudes e conspirações contra a Maçonaria, o imaginário popular ainda é constantemente alimentado por histórias e fantasias nascidas de lendas e suposições.
Full-length cDNAs: more than just reaching the ends (2001)

The development of functional genomic resources is essential to understand and utilize information generated from genome sequencing projects. Central to the development of this technology is the creation of high-quality cDNA resources and improved technologies for analyzing coding and noncoding mRNA sequences. The isolation and mapping of cDNAs is an entrée to characterizing the information that is of significant biological relevance in the genome of an organism. However, a bottleneck is often encountered when attempting to bring to full-length (or at least full-coding) a number of incomplete cDNAs in parallel, since this involves the nonsystematic, time consuming, and labor-intensive iterative screening of a number of cDNA libraries of variable quality and/or directed strategies to process individual clones (e.g., 5' rapid amplification of cDNA ends). Here, we review the current state of the art in cDNA library generation, as well as present an analysis of the different steps involved in cDNA library generation.
A monoclonal antibody specific for two-chain urokinase-type plasminogen activator. Application to the study of the mechanism of clot lysis with single-chain urokinase-type plasminogen activator in plasma (1990)

A murine monoclonal antibody (MA-12E6A8) was raised against human urokinase-type plasminogen activator (u-PA), which, in an enzyme-linked immunosorbent assay (ELISA), reacted 15,000-fold better with recombinant two-chain u-PA (rtcu-PA) than with recombinant single-chain u-PA (rscu-PA). The antibody had no effect on the activity of rtcu-PA or on its inhibition by a chloromethylketone, but reduced the inhibition of rtcu-PA by recombinant plasminogen activator inhibitor-1 (rPAI-1) at least 10-fold. The dissociation constant of the rtcu-PA/MA- 12E6A8 complex was 7 nmol/L. An ELISA was developed using MA-12E6A8 as capture antibody and a horseradish peroxidase conjugated u-PA specific antibody for tagging. It recognized free and active site blocked rtcu- PA but not rtcu-PA in complex with rPAI-1 or with alpha 2-antiplasmin. This ELISA was used to monitor the generation of rtcu-PA during fibrin clot lysis with rscu-PA in human plasma. Addition of 5 micrograms/mL rscu-PA to 3 mL plasma containing a 0.2 mL 125I-fibrin labeled plasma clot caused 50% clot lysis in 62 +/- 13 minutes (mean +/- SD, n = 6), at which time 99 +/- 28 ng/mL rtcu-PA was detected but no fibrinogen breakdown had occurred. Fifty percent fibrinogen breakdown did occur only when rtcu-PA had reached a level of 1,000 +/- 270 ng/mL (at 150 +/- 21 minutes). rscu-PA, 2 micrograms/mL, induced 50% clot lysis in 160 +/- 41 minutes (n = 6); no fibrinogen degradation occurred within 4 hours and rtcu-PA levels did not exceed 80 ng/mL. In the absence of a fibrin clot, 5 micrograms/mL rscu-PA added to human plasma did not result in significant generation of rtcu-PA (less than 50 ng/mL after 4 hours) and no fibrinogen degradation was observed. These results indicate that clot lysis with rscu-PA in a plasma milieu does not require extensive systemic conversion of rscu-PA to rtcu-PA, and that fibrinogen degradation occurs secondarily to systemic conversion of rscu-PA to rtcu-PA.
Suppression of Endogenous IL-10 Gene Expression in Dendritic Cells Enhances Antigen Presentation for Specific Th1 Induction: Potential for Cellular Vaccine Development (2000)

A new paradigm for designing vaccines against certain microbial pathogens, including Chlamydia trachomatis, is based on the induction of local mucosal Th1 response. IL-10 is an anti-inflammatory cytokine that exerts negative immunoregulatory influence on Th1 response. This study investigated whether biochemical modulation of endogenous IL-10 expression at the level of APCs is a practical strategy for enhancing the specific Th1 response against pathogens controlled by Th1 immunity. The results revealed that the high resistance of genetically engineered IL-10-/- (IL-10KO) mice to genital chlamydial infection is a function of the predilection of their APCs to rapidly and preferentially activate a high Th1 response. Thus, in microbiological analysis, IL-10KO mice suffered a shorter duration of infection, less microbial burden, and limited ascending infection than immunocompetent wild-type mice. Also, IL-10KO were resistant to reinfection after 8 wk of the primary infection. Cellular and molecular immunologic evaluation indicated that IL-10KO mice induced greater frequency of chlamydial-specific Th1 response following C. trachomatis infection. Moreover, IL-10KO APCs or antisense IL-10 oligonucleotide-treated wild-type APCs were potent activators of Th1 response from naive or immune T cells. Furthermore, both Ag-pulsed dendritic cells from IL-10KO mice and IL-10 antisense-treated dendritic cells from wild-type mice were efficient cellular vaccines in adoptive immunotherapeutic vaccination against genital chlamydial infection. These findings may furnish a novel immunotherapeutic strategy for boosting the Th1 response against T cell-controlled pathogens and tumors, using IL-10-deficient APCs as vaccine delivery agents.
Cyclo(L-leucyl-α,β-dehydrophenylalanine): the first diketopiperazine containing an α,β-dehydrophenylalanine residue (2005)

The title compound (systematic name: 3-benzylidene-6-isobutylpiperazine-2,5-dione), C15H18N2O2, an alpha,beta-dehydrophenylalanine containing diketopiperazine, crystallizes in the space group P1 with two molecules in the asymmetric unit arranged antiparallel to one another. The alpha,beta-dehydrophenylalanine (DeltaPhe) residue in this cyclic peptide retains its planarity but deviates from the standard conformations observed in its linear analogues. Each type of molecule forms a linear chain with molecules of the same type via pairwise N-H...O hydrogen bonds, while weaker C-H...O interactions link the chains together to form a three-dimensional network.
Modal Overlap and Dissipation Effects of a Cantilever Beam with Multiple Attached Oscillators (2000)

This work was prompted by a study performed by Strasberg [7] in which numerous small spring-mass-damper systems are attached to a large suspended mass representing the master structure. The isolated natural frequency of each attached system was selected to match in average the natural frequency of the isolated master structure. Strasberg found that the critical issue when an impulse excitation is applied to the master structure is the bandwidth of the isolated attached systems in comparison to the spacing between the natural frequencies of the system. Modal overlap, which corresponds to bandwidths that exceed the spacing of those frequencies, was shown to greatly influence the response of the master structure. Light damping, for which there is little or no modal overlap, corresponds to an impulse response that consists of a sequence of nearly periodic exponentially decaying pulses, and the transfer function for harmonic excitation of the master structure indicates that the substructure acts as a vibration absorber for the master structure. Increased damping leads to modal overlap, with the result that the impulse response consists of a single decaying pulse. The frequency domain transfer function indicates that the vibration absorber effect is enhanced. The present work explores these issues for continuous systems by replacing the one degree of freedom master structure with a cantilever beam. The system parameters are selected to match Strasberg’s model, with the suspended oscillators placed randomly along the beam. The beam displacement is represented as a Ritz series whose basis functions are the cantilever beam modes. The coupled equations are solved by a state-space eigenmode analysis that yields a closed form representation of the response in terms of the complex eigenmode properties. The continuous fuzzy structure is shown not to display the transfer of energy between the master structure and the substructure that was exhibited by the discrete fuzzy structure, apparently because of the asynchronous motion of the attachment points resulting from the spatial variability of the beam’s motion. The vibration absorber effect for harmonic excitation is only obtained for the heavy damping in the case of a beam.
Novel Anionic Oligomerization by a New, Sequential Generation of Organolithium Compounds (1996)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTNovel Anionic Oligomerization by a New, Sequential Generation of Organolithium CompoundsKeiji Maruoka, Masataka Oishi, and Hisashi YamamotoView Author Information School of Engineering, Nagoya University, Chikusa, Nagoya 464-01, Japan Cite this: Macromolecules 1996, 29, 9, 3328–3329Publication Date (Web):April 22, 1996Publication History Received13 November 1995Revised18 February 1996Published online22 April 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/ma951683jhttps://doi.org/10.1021/ma951683jrapid-communicationACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views245Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Anionic polymerization,Hydrocarbons,Oligomerization,Oligomers,Solvents Get e-Alerts
Answering Queries with Aggregation Using Views (1996)

We present novel algorithms for the problem of using materialized views to compute answers to SQL queries with grouping and aggregation, in the presence of multiset tables. In addition to its obvious potential in query optimization, this problem is important in many applications, such as data warehousing, very large transaction recording systems, global information systems and mobile computing, where access to local or cached materialized views may be cheaper than access to the underlying database. Our contributions are the following: First, we show that in the case where the query has grouping and aggregation but the views do not, a view is usable in answering a query only if there is an isomorphism between the view and a portion of the query. Second, when the views also have grouping and aggregation we identify conditions under which the aggregation information present in a view is sufficient to perform the aggregation computations required in the query. The algorithms we describe fo...
Recombinant glycoprotein product quality in proliferation-controlled BHK-21 cells (1999)

We analyzed product quality to determine the applicability of proliferation-controlled mammalian cells for recombinant pharmaceutical protein production. Baby hamster kidney (BHK)-21 cells were engineered to express a dicistronic, stabilized, self-selecting growth control system consisting of a beta-estradiol-activatable transcription factor IRF-1 fusion protein. IRF-1 activity led to a reduced growth rate, whereas productivity, protein integrity, and glycosylation pattern of the industrially relevant secreted pharmaceutical glycoprotein erythropoietin remained consistent, showing that this technique has the potential for improving the consistency of high-quality pharmaceutical products and thus warrants further development.
Water Soluble PDCA Derivatives for Selective Ln(III)/An(III) and Am(III)/Cm(III) Separation (2014)

The synthesis and solvent extraction behavior of dipicolinic acid (pyridine-2,6-dicarboxylic acid, PDCA) and their derivatives have been studied for possible use in selective back-extraction of actinides, especially americium. The extraction was performed from an organic phase containing a mixture of trivalent actinides and lanthanides pre-extracted with N,N,N’,N’-tetraoctyl diglycolamide (TODGA). The efficiency of the back-extraction was enhanced when the picolinate platform was used in a heterocyclic decadentate ligand called h4tpaen. Beyond selective An/Ln extraction, the aqueous soluble h4tpaen ligand seemed a potential reagent for an intra-group Am(III)/Cm(III) separation with a separation factor SFCm/Am of about 3.5.