gebhart/patrepeval huggingface dataset:
The IE2 regulatory protein of human cytomegalovirus induces expression of the human transforming growth factor beta1 gene through an Egr-1 binding site (1996)

Increases in transforming growth factor beta1 (TGF-beta1) mRNA and biological activity in the early phase of human cytomegalovirus (CMV) infection in fibroblasts are paralleled by increased TGF-beta1-chloramphenicol acetyltransferase (CAT) reporter gene activity. To determine how CMV infection transactivates the TGF-beta1 promoter, we examined the effects of the cotransfected IE2 regulatory protein of human CMV on 5'-deleted TGF-beta1 promoter-CAT reporter genes in transient DNA transfection assays. Two upstream TGF-beta1 promoter regions each containing an Egr-1 consensus site were shown to be important for IE2-induced transactivation in a cell type that displayed greatly reduced nonspecific activity. Furthermore, transfer of an Egr-l site from between positions -125 and -98, but not point mutant versions of this site, to a heterologous promoter also conveyed IE2 responsiveness. Addition of an IE2 expression vector or use of the U373 A45 astrocytoma cell line expressing IE2 also produced synergistic stimulation of GAL4-Egr-l-mediated activation of a target promoter containing GAL4 binding sites. The 80-kDa IE2 protein present in A45 cells proved to selectively bind to glutathione S-transferase (GST)-Egr-1 beads. The results of in vitro protein binding assays also revealed that an intact in vitro-translated IE2 protein bound directly to the GST-Egr-1 fusion protein through the zinc finger domain of the Egr-1 protein and that this binding activity was abolished by deletion of parts of the zinc finger DNA-binding domain. Similarly, the Egr-1 protein was found to associate preferentially with a small region within the C-terminal half of the IE2 protein adjacent to the DNA-binding and dimerization domains that are important for both transactivation and downregulation. We conclude from these observations that IE2 may regulate transcription of the TGF-beta1 gene as well as other potential cellular targets by virtue of its ability to interact with the Egr-1 DNA-binding protein.
Nestin expression in the cell lines derived from glioblastoma multiforme (2006)

Detailed information concerning the pattern of the nestin cytoskeleton in glioblastoma cell lines and especially the demonstration of nestin in the nucleus represent an important background for further studies of nestin re-expression in relationship to tumor malignancy and invasive potential.
Blood Endothelial Cells (2007)

In 1963, in an attempt to define the source of endothelium on vascular grafts, Stump and colleagues suspended a Dacron patch within the lumen of a prosthetic vascular graft in the aorta of a juvenile pig (1). As early as 14 days following placement, islands of endothelial cells (ECs) were identified on the patch surface. Because the patch had been isolated from contact with both the prosthesis and native vascular tissue, these findings implicated circulating blood as the source of ECs. These observations were not actively pursued from an experimental or clinical context until recently.
Fabrication and packaging of microbump interconnections for 3D TSV (2009)

Memory bandwidth has become a bottleneck to processor performance for tera-scale computing needs. To reduce this obstacle, a revolution in package technologies is required for tera-scale computing requirements. 3D TSV (Through Silicon Via) stacking is believed to be one of the technologies that can meet those requirements. In advanced 3D stacking technologies, one of the important steps is to develop and assemble fine pitch, high density solder microbumps. This type of solder microbump in flip chip interconnection provides a high wiring density in silicon die with a high-performance signal and power connection. There is a growing interest in the development and study of this new type of chip stacking and bonding approach for both existing and future devices. This paper will highlight the developments of ultra fine pitch and high density solder microbumps for advanced 3D stacking technologies. A Cu/SnAg solder microbump with 50/40 mum in pitch was fabricated at the silicon wafer level by an electroplating method. The total thickness of the plated Cu and SnAg microbump was 20 um. The under bump metallurgy (UBM) layer on the Si carrier used thin film based metal layers. The assembly of the Si chip and the Si carrier was conducted with the thermocompression flip chip bonder at different temperatures, times and pressures and the optimized bonding conditions were obtained. After assembly, the underfill process was carried out to fill the gap and achieve a void free underfilling using a material with a fine filler size. Finally, various reliability tests were carried out for mechanical characterization of microbump interconnections.
Reciprocal Activation of Transcription Factors Underlies the Dichotomy between Proliferation and Invasion of Glioma Cells (2013)

Histology of malignant glioma depicts dense proliferative areas rich in angiogenesis as well as dissemination of neoplastic cells into adjacent brain tissue. Although the mechanisms that trigger transition from proliferative to invasive phenotypes are complex, the dichotomy of cell proliferation and migration, the "Go or Grow" hypothesis, argues for specific and coordinated regulation of these phenotypes. We investigated transcriptional elements that accompany the phenotypes of migration and proliferation, and consider the therapeutic significance of the "Go or Grow" hypothesis. Interrogation of matched core and rim regions from human glioblastoma biopsy specimens in situ (n = 44) revealed higher proliferation (Ki67 labeling index) in cells residing at the core compared to the rim. Profiling activated transcription factors in a panel of migration-activated versus migration-restricted GBM cells portrayed strong NF-κB activity in the migratory cell population. In contrast, increased c-Myc activity was found in migration-restricted proliferative cells. Validation of transcriptional activity by NF-κB- or c-Myc-driven GFP or RFP, respectively, showed an increased NF-κB activity in the active migrating cells, whereas the proliferative, migration restricted cells displayed increased c-Myc activity. Immunohistochemistry on clinical specimens validated a robust phosphorylated c-Myc staining in tumor cells at the core, whereas increased phosphorylated NF-κB staining was detected in the invasive tumor cells at the rim. Functional genomics revealed that depletion of c-Myc expression by siRNA oligonucleotides reduced cell proliferation in vitro, but surprisingly, cell migration was enhanced significantly. Conversely, inhibition of NF-κB by pharmacological inhibitors, SN50 or BAY-11, decreased both cell migration in vitro and invasion ex vivo. Notably, inhibition of NF-κB was found to have no effect on the proliferation rate of glioma cells. These findings suggest that the reciprocal and coordinated suppression/activation of transcription factors, such as c-Myc and NF-κB may underlie the shift of glioma cells from a "growing-to-going" phenotype.
Synthesis and anticonvulsant activity of 1-phenylcyclohexylamine analogs (1990)

Thirty-eight analogues of 1-phenylcyclohexylamine (PCA), a phencyclidine (PCP) derivative, were examined for their activities in the mouse maximal electroshock (MES) seizure test and in a motor-toxicity assay. In addition, we determined the binding affinities of the compounds for PCP acceptor sites in rat brain membranes labeled with [3H]-1-[1-(2-thienyl)cyclohexyl]piperidine. Many of the analogues were protective against MES seizures (ED50s of 5-41 mg/kg, ip) and all of these compounds caused motor toxicity. The potencies in the motor toxicity and MES seizure tests showed a moderate correlation with the affinities for PCP sites. Several analogues exhibited a greater separation of potencies in the motor toxicity and MES seizure tests than did the parent compound PCA. These were obtained by (i) 3-methylation of the cyclohexyl ring trans to the phenyl ring, (ii) methoxylation at the ortho position on the phenyl ring, and (iii) contraction of the cyclohexane ring to form the corresponding cyclopentane.
The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. (1984)

A murine hybridoma cell line was obtained producing monoclonal antibody specific for human beta 2-microglobulin (beta 2m). The antibody reacts strongly with cell-associted human beta 2m, weakly with the cells of the monkey Cercopithecus aethiops, but not with any other non-primate cells tested. It is of IgG2a isotype, its pI is 6.9-7.1 and dissociation constant of its complex with soluble beta 2m is K = 1.5 X 10(-8) mol/l. The antibody also binds to beta 2m after electrophoretic separation under the reducing and denaturating conditions and blotting onto nitrocellulose. Immobilization of this antibody on Sepharose 4B yields an efficient immunosorbent for purification of beta 2m.
Prognostic value of bone marrow angiogenesis in multiple myeloma. (2000)

We studied the prognostic value of angiogenesis grading and microvessel density estimation in newly diagnosed multiple myeloma. Seventy-five patients with newly diagnosed myeloma, treated on Eastern Cooperative Oncology Protocol E9486 and Intergroup study 0141 (S9321) at the Mayo Clinic, were studied. Bone marrow microvessels were examined using immunohistochemical staining for von Willebrand factor. Determination of microvessel density and angiogenesis grading was done in a blinded manner. There was a strong correlation between microvessel density and the plasma cell labeling index, rho 0.42, P < 0.001. Angiogenesis grade was also significantly associated with the plasma cell labeling index. Fifteen % of patients with low-grade angiogenesis had a high labeling index (>1%). In contrast, 47% of patients with intermediate or high-grade angiogenesis had high labeling indices (P = 0.02). Overall survival was significantly different among those with high-, intermediate-, and low-grade angiogenesis, with median times of 2, 4, and 4.4 years, respectively (P = 0.02). Similarly, patients with microvessel density >50/x400 field had poorer survival compared with those with 50 or fewer microvessels/field, median survival 2.6 versus 5.1 years, respectively (P = 0.004). There was a strong association between angiogenesis grade and microvessel density (P < 0.001). We conclude that bone marrow angiogenesis is a predictor of poor survival in newly diagnosed myeloma. Angiogenesis is correlated with the plasma cell labeling index but not the bone marrow plasma cell percentage. A simple visual grading of angiogenesis is an efficient alternative to microvessel density estimation.
Lovastatin inhibits tumor growth and lung metastasis in mouse mammary carcinoma model: a p53-independent mitochondrial-mediated apoptotic mechanism (2004)

The effects of lovastatin, a potent inhibitor of hydroxymethylglutaryl-coenzyme A reductase, were studied in a mouse model of metastatic mammary cancer carrying a p53 mutation. Mice bearing mammary tumors, induced by inoculation of syngeneic BALB/c mice with BJMC3879 cells, were treated with lovastatin at 0, 25 and 50 mg/kg three times a week. Tumor volumes were significantly reduced in a dose-dependent manner throughout the 6 week study and were associated with both a decrease in DNA synthesis and an increase in apoptosis. The high dose of lovastatin also inhibited lung metastasis. In a corollary in vitro study, flow cytometric analyses of lovastatin-treated mammary cancer cells additionally showed cell cycle arrest at G1 phase and decreases in S and G2/M phases. Laser scanning cytometric analyses further demonstrated that cancer cells in S and G2/M were particularly susceptible to the effects of lovastatin. Transmission electron microscopic evaluation of TUNEL-confirmed apoptotic bodies in lovastatin-treated mammary carcinoma cells revealed many free 3'-OH ends of DNA in condensed chromatin within fragmented nuclei that occasionally assumed a characteristic half-moon shape. Consistent with initiation of apoptosis, cellular caspase-8, caspase-9 and caspase-3 activities were elevated in lovastatin-treated cells. The mitochondrial membrane potential was also decreased, with subsequent release of cytochrome c. However, lovastatin-induced cell death was significantly reduced by the broad spectrum caspase inhibitor z-VAD-fmk, as well as the caspase-9 inhibitor z-LEHD-fmk and the caspase-3 inhibitor z-DEVD-fmk, but not by the specific caspase-8 inhibitor z-IETD-fmk. Since immunoelectron microscopy showed translocation of Bax to the mitochondria in lovastatin-treated cells, lovastatin-induced apoptosis may, therefore, be ultimately dependent on Bax induction of cytochrome c release. These results suggest that lovastatin may be useful as an adjuvant therapy in breast cancers containing p53 mutations due to its ability to both suppress DNA synthesis and induce p53-independent mitochondria-mediated apoptosis.
In vivo gene electroporation in skeletal muscle with special reference to the duration of gene expression. (2001)

To determine the limits of the duration of in vivo transferred foreign gene expression, we conducted electroporation (EP), a powerful non-viral means of gene transfer for living animals, into skeletal muscle of rats and mice with a luciferase, GFP or erythropoietin (EPO)-encoding reporter plasmid. The luciferase reporter plasmid was used for optimization of EP conditions, while GFP and EPO plasmids were used for monitoring the duration of gene expression. In the rat, increased hematocrit levels were maintained for at least 9 weeks with approximately a 3-fold increase in plasma EPO protein concentration at 4 weeks post-transfection. In the mouse, the GFP plasmid transfer confirmed that the reporter gene expression lasted as long as 3 months post-transfection. By introducing the EPO gene in vivo in the mouse, increased hematocrit levels revealed that duration of reporter gene expression was at least 14.5 months after in vivo gene EP into skeletal muscle. These results implicate an excellent potential of in vivo gene EP, applicable to both experimental and therapeutic purposes.
The Role of Zinc Binding in the Biological Activity of Botulinum Toxin (2001)

Botulinum toxin is a zinc-dependent endoprotease that acts on vulnerable cells to cleave polypeptides that are essential for exocytosis. To exert this poisoning effect, the toxin must proceed through a complex sequence of events that involves binding, productive internalization, and intracellular expression of catalytic activity. Results presented in this study show that soluble chelators rapidly strip Zn(2+) from its binding site in botulinum toxin, and this stripping of cation results in the loss of catalytic activity in cell-free or broken cell preparations. Stripped toxin is still active against intact neuromuscular junctions, presumably because internalized toxin binds cytosolic Zn(2+). In contrast to soluble chelators, immobilized chelators have no effect on bound Zn(2+), nor do they alter toxin activity. The latter finding is because of the fact that the spontaneous loss of Zn(2+) from its coordination site in botulinum toxin is relatively slow. When exogenous Zn(2+) is added to toxin that has been stripped by soluble chelators, the molecule rebinds cation and regains catalytic and neuromuscular blocking activity. Exogenous Zn(2+) can restore toxin activity either when the toxin is free in solution on the cell exterior or when it has been internalized and is in the cytosol. The fact that stripped toxin can reach the cytosol means that the loss of bound Zn(2+) does not produce conformational changes that block internalization. Similarly, the fact that stripped toxin in the cytosol can be reactivated by ambient Zn(2+) or exogenous Zn(2+) means that productive internalization does not produce conformational changes that block rebinding of cation.
Surgical margins and survival after head and neck cancer surgery (2006)

Our findings suggest that positive surgical margin status is associated with increased mortality. This association also generally persists after adjustment for tumor size, stage, and adjuvant therapy.
People Identification with Limited Labels in Privacy-Protected Video (2006)

People identification is an essential task for video content analysis in a surveillance system. To construct a good classifier requires a large amount of training data, which may not be obtained in some scenario. In this paper, we propose an approach to augment insufficient training data by labeling identical video images that have removed people's identities by masking faces. We show user study results that human subjects can perform reasonably well in labeling pairwise constraints from face obscured images. We also present a new discriminative learning algorithm WPKLR to handle uncertainties in pairwise constraints. The effectiveness of the proposed approach is demonstrated using video captured in a nursing home environment. The experiments show that the WPKLR approach can obtain a high accuracy of people identification using limited labeled data and noisy pairwise constraints, and meanwhile minimize the risk of exposing people's identities
Screening for Ovarian Cancer (1994)

Available evidence does not support either screening of pre- or postmenopausal women without a family history of ovarian cancer or routine screening in women with a family history of ovarian cancer in one or more relatives (without evidence of a hereditary cancer syndrome). Women from a family with the rare hereditary ovarian cancer syndrome are at high risk for the disease and should be referred to a gynecologic oncologist.
Lipids of Bovine and Human Milks: A Comparison (1990)

Human and bovine milks contain about 3 to 5% total lipid, existing as emulsified globules 2 to 4 microns in diameter and coated with a membrane derived from the secreting cell. About 98% or more of the lipid is triacylglycerol, which is found in the globule. Phospholipids are about .5 to 1% of total lipids and sterols are .2 to .5%; these are mostly located in the globule membrane. Cholesterol is the major sterol. The major differences are in fatty acid composition, triacylglycerol structure, and the response of fatty acids in human milk to changes in diet. Bovine milk contains substantial quantities of 4:0 to 10:0, about 2% 18:2, and almost no other long-chain polyunsaturated fatty acids. The fatty acid composition is not altered by ordinary changes in diet. Human milk contains very little 4:0 to 10:0, 10 to 14% 18:2, and small quantities of other polyunsaturates. The triacylglycerol structure differs, with much of the sn-2 position occupied by 16:0 in human milk and 4:0 to 10:0 at sn-3 in bovine milk. The effects of milk cholesterol and fatty acids on human blood cholesterol levels are discussed.
Oil Industry First Interwell Trial of Reservoir Nanoagent Tracers (2016)

Abstract This manuscript reports the industry's first proven reservoir nanoagents' design and describes a successful multi-well field trial using these inexpensive and environmentally friendly nanoparticles that offer an important advantage of fast and cheap fluorometric detection. Our fundamental nanoparticle tracer template, A-Dots or Arab-D dots, is intentionally geared towards the harsh but prolific Arab-D carbonate reservoir environment of 100°C temperature, 150,000 ppm salinity, and an abundant presence of divalent ions in the connate water. The A-Dots were manufactured on a scale of one metric ton from affordable and easily available commodity chemicals. They were injected into a watered-out part of the field and monitored at four nearby producer wells for two years. Monitoring of four neighbouring producer wells over a period of 26 months confirmed nanoparticles' breakthrough at a single producer nearly 500 m from the injector at the reservoir level, thus, proving the nanoparticles' mobility and transport capability. The maximum concentration of the nanoagent in produced water was observed about 10 months after the injection matching the behavior of conventional small-molecule tracers used in the same pair of wells previously. The rate of A-Dots production correlated with the rate of water injection at the original injector well and followed it closely with a 10-month delay. This test bolstered our previous observations of satisfactory recovery of A-Dots in a single-well test by confirming their reservoir stability on industry relevant time scales and demonstrating the feasibility of their industrial production. The importance of this accomplishment is not in how sophisticated the sensing functionality of the tracer design is but rather in the nanoparticle stability, mobility, scalability, and field application potentials. Our findings render the concept of having active, reactive, and even communicative, in-situ reservoir nanoagents for underground sensing and intervention a well anticipated near-future reality.
Polyhedra Self-Assembled from DNA Tripods and Characterized with 3D DNA-PAINT (2014)

DNA self-assembly has produced diverse synthetic three-dimensional polyhedra. These structures typically have a molecular weight no greater than 5 megadaltons. We report a simple, general strategy for one-step self-assembly of wireframe DNA polyhedra that are more massive than most previous structures. A stiff three-arm-junction DNA origami tile motif with precisely controlled angles and arm lengths was used for hierarchical assembly of polyhedra. We experimentally constructed a tetrahedron (20 megadaltons), a triangular prism (30 megadaltons), a cube (40 megadaltons), a pentagonal prism (50 megadaltons), and a hexagonal prism (60 megadaltons) with edge widths of 100 nanometers. The structures were visualized by means of transmission electron microscopy and three-dimensional DNA-PAINT super-resolution fluorescent microscopy of single molecules in solution.
A simplified twenty‐eight–joint quantitative articular index in rheumatoid arthritis (1989)

We describe a joint index that includes only 28 joints: 10 proximal interphalangeal joints of the fingers, 10 metacarpophalangeal joints, and the wrists, elbows, shoulders, and knees. These joints are evaluated for swelling, tenderness, and limited motion, with the findings scored as abnormal or normal. The 28-joint index is considerably easier to use than traditional methods, and appears to yield as much information in terms of correlation with other measures of clinical status, including hand radiograph scores, American Rheumatism Association functional class, grip strength, walking time, and activities of daily living questionnaire scores (r = 0.25-0.53, P less than 0.001). Simplified joint counts might facilitate acquisition of quantitative articular data in research and clinical rheumatologic settings.
MARKETING AND THE DEMOGRAPHIC PERSPECTIVE (1986)

Demographic data have proven quite useful in the structuring of some marketing strategies. However, the development of a demographic perspective, which would extend the use of what marketers call demographics, has not been forthcoming. This article offers both an explanation of the demographic perspective—how it differs from demographics—along with examples of how this perspective can make demographic data more useful to marketers.
Why do veins appear blue? A new look at an old question (1996)

We investigate why vessels that contain blood, which has a red or a dark red color, may look bluish in human tissue. A CCD camera was used to make images of diffusely reflected light at different wavelengths. Measurements of reflectance that are due to model blood vessels in scattering media and of human skin containing a prominent vein are presented. Monte Carlo simulations were used to calculate the spatially resolved diffuse reflectance for both situations. We show that the color of blood vessels is determined by the following factors: (i) the scattering and absorption characteristics of skin at different wavelengths, (ii) the oxygenation state of blood, which affects its absorption properties, (iii) the diameter and the depth of the vessels, and (iv) the visual perception process.