gebhart/patrepeval huggingface dataset:
Induction of CD45 Expression and Proliferation in U-266 Myeloma Cell Line by Interleukin-6 (1998)

Recently, there has been an increasing interest in the expression pattern and biological significance of the CD45 molecule in myeloma cells. In this study, we have further defined the phenotypic pattern of CD45 expression on myeloma cells. Using a panel of myeloma cell lines, we showed that CD45 showed a remarkably heterogeneous pattern of expression. Whereas some cell lines were CD45+ and others were CD45−, the U-266 cell line, although predominantly CD45−, still had a considerable subpopulation of CD45+ cells. Among the myeloma cell lines examined, there was a direct correlation between interleukin-6 (IL-6) dependency and CD45 positivity. Moreover, we showed that IL-6 stimulation led to the induction of expression of CD45 and cellular proliferation. Using independent experimental approaches, we could show that the IL-6–induced effects were closely linked to CD45 expression. First, sorting out CD45+ and CD45− subsets of U-266 cell line followed by IL-6 stimulation, only the CD45+ cells showed a proliferative advantage after IL-6 stimulation. Second, IL-6 stimulation of sorted CD45−cells was gradually followed by phenotypic conversion to CD45+ cells that started after 2 days as judged by the detection of CD45 mRNA by reverse transcription polymerase chain reaction (RT-PCR) and immunophenotypic analysis by flow cytometry. Withdrawal of IL-6 from the medium led to gradual loss of CD45 expression in CD45+ flow-sorted U-266 cells. Third, the use of vanadate, a potent inhibitor of protein tyrosine phosphatase (PTP), abrogated the IL-6–induced proliferation in the CD45+ myeloma cells. On the other hand, cellular proliferation induced by IL-6 was not affected by the serine-threonine phosphatase inhibitor okadaic acid. Our data show that the expression pattern of CD45 in myeloma cell lines is heterogeneous and show for the first time that CD45 expression can be induced by IL-6 stimulation. Finally, these data shed some light on the biological role of CD45 in myeloma by determining the proliferative population among myeloma cells.
Post Office Protocol - Version 3 (1993)

The Post Office Protocol - Version 3 (POP3) is intended to permit a
workstation to dynamically access a maildrop on a server host in a
useful fashion. [STANDARDS-TRACK]
Hypertension and Antihypertensive Therapy as Risk Factors for Type 2 Diabetes Mellitus (2000)

Concern about the risk of diabetes should not discourage physicians from prescribing thiazide diuretics to nondiabetic adults who have hypertension. The use of beta-blockers appears to increase the risk of diabetes, but this adverse effect must be weighed against the proven benefits of beta-blockers in reducing the risk of cardiovascular events.
Audio Classification Based on MPEG-7 Spectral Basis Representations (2004)

In this paper, we present an MPEG-7-based audio classification and retrieval technique targeted for analysis of film material. The technique consists of low-level descriptors and high-level description schemes. For low-level descriptors, low-dimensional features such as audio spectrum projection based on audio spectrum basis descriptors is produced in order to find a balanced tradeoff between reducing dimensionality and retaining maximum information content. High-level description schemes are used to describe the modeling of reduced-dimension features, the procedure of audio classification, and retrieval. A classifier based on continuous hidden Markov models is applied. The sound model state path, which is selected according to the maximum-likelihood model, is stored in an MPEG-7 sound database and used as an index for query applications. Various experiments are presented where the speaker- and sound-recognition rates are compared for different feature extraction methods. Using independent component analysis, we achieved better results than normalized audio spectrum envelope and principal component analysis in a speaker recognition system. In audio classification experiments, audio sounds are classified into selected sound classes in real time with an accuracy of 96%.
An adaptive low-power transmission scheme for on-chip networks (2002)

Systems-on-Chip (SoC) are evolving toward complex heterogeneous multiprocessors made of many predesigned macrocells or subsystems with application-specific interconnections. Intra-chip interconnects are thus becoming one of the central elements of SoC design and pose conflicting goals in terms of low energy per transmitted bit, guaranteed signal integrity, and ease of design. This work introduces and shows first results on a novel interconnect system which uses low-swing signalling, error detection codes, and a retransmission scheme; it minimises the interconnect voltage swing and frequency subject to workload requirements and S/N conditions. Simulation results show that tangible savings in energy can be attained while achieving at the same time more robustness to large variations in actual workload, noise, and technology quality (all quantities easily mispredicted in very complex systems and advanced technologies). It can be argued that traditional worst-case correct-by-design paradigm will be less and less applicable in future multibillion transistor SoC and deep sub-micron technologies; this work represents a first example towards robust adaptive designs.
The aldo-keto reductase AKR1C3 is a novel suppressor of cell differentiation that provides a plausible target for the non-cyclooxygenase-dependent antineoplastic actions of nonsteroidal anti-inflammatory drugs. (2003)

We and others have demonstrated expression of the aldo-keto reductase AKR1C3 in myeloid leukemia cell lines and that inhibitors of the enzyme, including nonsteroidal anti-inflammatory drugs (NSAIDs), promote HL-60 differentiation in response to all-trans retinoic acid (ATRA) and 1alpha,25-dihydroxyvitamin D3 (D3). Here, we demonstrate that overexpression of AKR1C3 reciprocally desensitizes HL-60 cells to ATRA and D3, thus confirming the enzyme as a novel regulator of cell differentiation. AKR1C3 possesses marked 11-ketoreductase activity converting prostaglandin (PG) D2 to PGF2alpha. Supplementing HL-60 cultures with PGD2 mimicked treatment with AKR1C3-inhibitors by enhancing the differentiation of the cells in response to ATRA. However, PGD2 is chemically unstable, being converted first to PGJ2 and then stepwise to 15-deoxy-Delta(12,14)-prostaglandin J2(15Delta-PGJ2), a natural ligand for the peroxisome proliferator-activated receptor-gamma (PPARgamma). Consistent with this, PGD2 was rapidly converted to PGJ2 under normal tissue culture conditions but not in the presence of recombinant AKR1C3 when PGF2alpha was predominantly formed. In addition, PGJ2 but not PGF2alpha recapitulated the potentiation of HL-60 differentiation by PGD2 and AKR1C3 inhibitors. Furthermore, the capacity of all of these treatments to potentiate HL-60 cell differentiation was significantly reduced in the presence of the PPARgamma-antagonist GW 9662. We conclude that AKRIC3 protects HL-60 cells against ATRA and D3-induced cell differentiation by limiting the production of natural PPARgamma ligands via the diversion of PGD2 toward PGF2alpha and away from PGJ2. In addition, these observations identify AKR1C3 as plausible target for the non-cyclooxygenase-dependent antineoplastic actions of NSAIDs.
Inhibitory effect of aspirin on azoxymethane-induced colon carcinogenesis in F344 rats (1993)

Epidemiologic studies suggest that sustained use of aspirin may reduce the risk of development of and mortality due to colon cancer. Previous preclinical studies have shown that several non-steroidal anti-inflammatory drugs act as potential chemopreventive agents in experimentally induced colon cancer models. The present study was designed to investigate the chemopreventive effect of 40 and 80% maximum tolerated dose (MTD) levels of aspirin administered in the diet on azoxymethane (AOM)-induced colon carcinogenesis in male F344 rats. The MTD of aspirin as determined in male F344 rats was 500 p.p.m. Beginning at 5 weeks of age, all animals were randomly divided into various experimental groups (48 rats/group) and fed one of the semipurified diets containing 0, 200 p.p.m. (40% MTD), or 400 ppm (80% MTD) of aspirin. Two weeks later, all animals (36 rats/group) except the vehicle-treated groups (12 rats/group) were administered s.c. injections of AOM at a dose level of 15 mg/kg body wt, once weekly for 2 weeks. All animals were continued on their respective dietary regimen for additional 52 weeks and necropsied. Histopathologic evaluation of colon tumors was performed by routine procedures. Basal levels and ex vivo production of colonic mucosal and tumor prostaglandin E2 (PGE2) were measured in all groups. The results indicate that daily oral administration of 200 and 400 p.p.m. aspirin significantly inhibited the incidence (% animals with tumors) and multiplicity (tumors/animal) of invasive adenocarcinomas of the colon as well as the size of adenocarcinomas. Colonic mucosal and tumor PGE2 levels (basal and ex vivo production) were significantly reduced in animals administered 200 and 400 p.p.m. aspirin. The results of this study support the epidemiologic evidence that ingestion of aspirin inhibits colon carcinogenesis. Although the precise mechanisms of aspirin-induced colon tumor inhibition remain to be determined, it is likely that the effect may be mediated through the modulation of prostaglandin synthesis.
BglBrick vectors and datasheets: A synthetic biology platform for gene expression (2011)

The standardized collection of vectors presented here allows the user to rapidly construct and test the expression of genes with various combinations of promoter strength, inducible expression system, copy number, and antibiotic resistance. The quantitative datasheets created for these vectors will increase the predictability of gene expression, especially when multiple plasmids and inducers are utilized.
Adenosine A2b receptors evoke interleukin-8 secretion in human mast cells. An enprofylline-sensitive mechanism with implications for asthma. (1995)

Adenosine potentiates mast cell activation, but the receptor type and molecular mechanisms involved have not been defined. We, therefore, investigated the effects of adenosine on the human mast cell line HMC-1. Both the A2a selective agonist CGS21680 and the A2a/A2b nonselective agonist 5'-N-ethylcarboxamidoadenosine (NECA) increased cAMP, but NECA was fourfold more efficacious and had a Hill coefficient of 0.55, suggesting the presence of both A2a and A2b receptors. NECA 10 microM evoked IL-8 release from HMC-1, but CGS21680 10 microM had no effect. In separate studies we found that enprofylline, an antiasthmatic previously thought to lack adenosine antagonistic properties, is as effective as theophylline as an antagonist of A2b receptors at concentrations achieved clinically. Both theophylline and enprofylline 300 micro completely blocked the release of IL-8 by NECA. NECA, but not CGS21680, increases inositol phosphate formation and intracellular calcium mobilization through a cholera and pertussis toxin-insensitive mechanism. In conclusion, both A2a and A2b receptors are present in HMC-1 cells and are coupled to adenylate cyclase. In addition, A2b receptors are coupled to phospholipase C and evoke IL-8 release. This effect is blocked by theophylline and enprofylline, raising the possibility that this mechanism contributes to their antiasthmatic effects.
A study to evaluate the effects of intranasal dexmedetomidine as a premedicant in paediatric patients undergoing cardiac surgeries (2015)

Background Premedication is defined as the administration of drugs before anaesthesia to allay apprehension, produce sedation and facilitate the administration of anaesthesia to the patient. The current study is a prospective randomized double-blinded trail conducted to evaluate the effects of intranasal dexmedetomidine over placebo (0.9% saline) as a premedicant in paediatric cardiac surgeries. Patients and methods A total of 60 children of ASA physical status I and II, between the ages of 2 and 8 years undergoing various cardiac surgeries were assigned randomly into two groups. In group D, patients received intranasal dexmedetomidine (2 μg/kg), and in group P, patients received intranasal saline. At intervals of 10, 20, 30 and 45 min after intranasal administration of the study drug, parameters such as heart rate, blood pressure, respiratory rate and SpO 2 (oxygen saturation) were monitored. At 45 min, sedation, the ease of separation and intravenous cannula acceptance were evaluated. Results Statistically significant reductions in heart rate and blood pressure were observed from 30 min onwards in group D (P < 0.05) when compared with the placebo group. In the study, the sedation score in group D was 3.23 ± 0.568 when compared with 1.13 ± 0.345 in the placebo group (P = 0.0001). The ease of parental separation was 2.63 ± 0.614 in group D compared with 1.1 ± 0.305 in the saline group (P = 0.0001). The intravenous cannula acceptance score in group D was 2.1 ± 0.547 when compared with 1.06 ± 0.253 in group P (P = 0.0001). ConclusionIntranasal dexmedetomidine (2 μg/kg) provided better sedation, parental separation and intravenous cannula acceptance than placebo in children undergoing cardiac surgeries.
Implication of neutral polysaccharides associated to alginate in inhibition of murine macrophage response to Pseudomonas aeruginosa (2006)

There is evidence that exopolysaccharides (EPS) contribute to the persistence of Pseudomonas aeruginosa in cystic fibrosis lung. However, the relationship between the chemical composition of EPS and the modulation of phagocytic cells is poorly understood. In order to evaluate the role of the chemical composition of EPS in macrophage behavior changes, we pretreated macrophages with characterized EPS and assessed P. aeruginosa phagocytosis and reactive oxygen intermediate (ROI) production. The results showed that alginate and neutral polysaccharides are involved in phagocytic impairment of P. aeruginosa. Moreover, alginates were able to prime macrophages for increased P. aeruginosa-induced macrophage oxidative burst as determined by chemiluminescence. In contrast, neutral polysaccharides are responsible for the decrease of ROI by a scavenging effect evaluated by the xanthine-xanthine oxidase system. This study showed that the content of P. aeruginosa EPS in alginate, but also in neutral polysaccharides, influences the behavior of strains towards phagocytosis and macrophage oxidative burst.
Ribonuclease H: the enzymes in eukaryotes (2009)

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. There are two main types of RNase H, and at least one of them is present in most organisms. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts. Eukaryotic RNase H1 has acquired a hybrid binding domain that confers processivity and affinity for the substrate, whereas eukaryotic RNase H2 is composed of three different proteins: the catalytic subunit (2A), similar to the monomeric prokaryotic RNase HII, and two other subunits (2B and 2C) that have no prokaryotic counterparts and as yet unknown functions, but that are necessary for catalysis. In this minireview, we discuss some of the most recent findings on eukaryotic RNases H1 and H2, focusing on the structural data on complexes between human RNase H1 and RNA/DNA hybrids that had provided great detail of how the hybrid binding- and RNase H-domains recognize and cleave the RNA strand of the hybrid substrates. We also describe the progress made in understanding the in vivo function of eukaryotic RNases H. Although prokayotes and some single-cell eukaryotes do not require RNases H for viability, in higher eukaryotes RNases H are essential. Rnaseh1 null mice arrest development around E8.5 because RNase H1 is necessary during embryogenesis for mitochondrial DNA replication. Mutations in any of the three subunits of human RNase H2 cause Aicardi-Goutières syndrome, a human neurological disorder with devastating consequences.
Activation of intrinsic blood coagulation by ellagic acid: insoluble ellagic acid-metal ion complexes are the activating species (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTActivation of intrinsic blood coagulation by ellagic acid: insoluble ellagic acid-metal ion complexes are the activating speciesPaul E. Bock, K. R. Srinivasan, and Joseph D. ShoreCite this: Biochemistry 1981, 20, 25, 7258–7266Publication Date (Print):December 1, 1981Publication History Published online1 May 2002Published inissue 1 December 1981https://pubs.acs.org/doi/10.1021/bi00528a032https://doi.org/10.1021/bi00528a032research-articleACS PublicationsRequest reuse permissionsArticle Views697Altmetric-Citations60LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
An Investigation of the Effect of Carbon Black Structure, Polymer Morphology, and Processing History on the Electrical Conductivity of Carbon‐Black‐Filled Thermoplastics (1984)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation D. M. Bigg; An Investigation of the Effect of Carbon Black Structure, Polymer Morphology, and Processing History on the Electrical Conductivity of Carbon‐Black‐Filled Thermoplastics. J. Rheol. 1 October 1984; 28 (5): 501–516. https://doi.org/10.1122/1.549758 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentThe Society of RheologyJournal of Rheology Search Advanced Search |Citation Search
Evaluation and Treatment of Hypertriglyceridemia: An Endocrine Society Clinical Practice Guideline (2012)

The Task Force recommends that the diagnosis of hypertriglyceridemia be based on fasting levels, that mild and moderate hypertriglyceridemia (triglycerides of 150-999 mg/dl) be diagnosed to aid in the evaluation of cardiovascular risk, and that severe and very severe hypertriglyceridemia (triglycerides of > 1000 mg/dl) be considered a risk for pancreatitis. The Task Force also recommends that patients with hypertriglyceridemia be evaluated for secondary causes of hyperlipidemia and that subjects with primary hypertriglyceridemia be evaluated for family history of dyslipidemia and cardiovascular disease. The Task Force recommends that the treatment goal in patients with moderate hypertriglyceridemia be a non-high-density lipoprotein cholesterol level in agreement with National Cholesterol Education Program Adult Treatment Panel guidelines. The initial treatment should be lifestyle therapy; a combination of diet modification and drug therapy may also be considered. In patients with severe or very severe hypertriglyceridemia, a fibrate should be used as a first-line agent.
Data logging technology in ambulatory medical instrumentation (2001)

This paper reviews the advancements made in ambulatory data logging used in the study of human subjects since the inception of the analogue tape based data logger in the 1960s. Research into the area of ambulatory monitoring has been rejuvenated due to the development of novel storage technologies during the 1990s. Data logging systems that were previously impractical due to lack of processing power, practical size and cost are now available to the practitioner. An overview of the requirements of present day ambulatory data logging is presented and analogue tape, solid-state memory and disk drive storage recording systems that have been described in the literature are investigated in detail. It is proposed that digital based technology offers the best solution to the problems encountered during human based data logging. The appearance of novel digital storage media will continue the trend of increased recording durations, signal resolution and number of parameters thus allowing the momentum gained throughout the last several decades to continue.
The unique rht-MOF platform, ideal for pinpointing the functionalization and CO2adsorption relationship (2011)

The uniqueness of the rht-MOF platform, based on the singular (3,24)-connected net, allows for the facile design and synthesis of functionalized materials for desired applications. Here we designed a nitrogen-rich trefoil hexacarboxylate (trigonal tri-isophthalate) ligand, which serves to act as the trigonal molecular building block while concurrently coding the formation of the targeted truncated cuboctahedral supermolecular building block (in situ), and enhancing the CO(2) uptake in the resultant rht-MOF.
Colorimetric Sensor Arrays for Volatile Organic Compounds (2006)

The development of a low-cost, sensitive colorimetric sensor array for the detection and identification of volatile organic compounds (VOCs) is reported. Using an array composed of chemoresponsive dyes, enormous discriminatory power is possible in a simple device that can be imaged easily with an ordinary flatbed scanner. Excellent differentiation of closely related organic compounds can be achieved, and a library of 100 VOCs is presented. The array discriminates among VOCs by probing a wide range of intermolecular interactions, including Lewis acid/base, Brønsted acid/base, metal ion coordination, hydrogen bonding, and dipolar interactions. Importantly, by proper choice of dyes and substrate, the array is essentially nonresponsive to changes in humidity.
Light Sensitive Chloramphenicol Analogues. (1975)

Some chloramphenicol analogues have been prepared in order to find reagents which could be used in a photoaffinity labelling study of the peptidayl transferase center of the E. coli ribosome. The compounds were tested for antibiotic activity in vitro in the "fragment reaction" assay, and for their ability to generate very reactive species upon irradiation.