gebhart/patrepeval huggingface dataset:
A phase I study of bolus versus continuous infusion of the anti-CD19 immunotoxin, IgG-HD37-dgA, in patients with B-cell lymphoma (1996)

IgG-HD37-SMPT-dgA is a deglycosylated ricin A chain (dgA)-containing immunotoxin (IT) prepared by conjugating the monoclonal murine (MoAb) anti-CD19 antibody, HD37, to dgA using the heterobifunctional hindered disulfide linker, N-succinimidyl-oxycarbonyl-alpha-methyl-alpha-(2-pyridyldithio) toluene (SMPT). In this report, we have used two regimens for the administration of IgG-HD37-SMPT-dgA to patients with non-Hodgkin's lymphoma (NHL) in two concomitant phase I trials. One trial examined four intermittent bolus infusions administered at 48-hour intervals. The other studied a continuous infusion (CI) administered over the same 8-day period. In the intermittent bolus regimen, the maximum tolerated dose (MTD) was 16 mg/m2/8 d and the dose-limiting toxicity (DLT) consisted of vascular leak syndrome (VLS), aphasia, and evidence of rhabdomyolysis encountered at 24 mg/m2/8 d. Using the CI regimen, the MTD was defined by VLS at 19.2 mg/m2/8 d. At the MTD of both regimens, a novel toxicity, consisting of acrocyanosis with reversible superficial distal digital skin necrosis in the absence of overt evidence of systemic vasculitis, occurred in 3 patients. Of 23 evaluable patients on the bolus schedule, there was 1 persisting complete response (CR; > 40 months) and 1 partial response (PR). Of 9 evaluable patients on the continuous infusion regimen, there was 1 PR. Pharmacokinetic parameters for the bolus regimen at the MTD showed a mean maximum serum concentration (Cmax) of 1,209 +/- 430 ng/mL, with a median T1/2 beta for all courses of 18.2 hours (range, 10.0 to 80.0 hours), a volume of distribution (Vd) of 10.9 L (range, 3.1 to 34.5 L), and a clearance (CL) of 0.45 L/h (range, 0.13 to 2.3 L/h). For the CI regimen at MTD, the mean Cmax was 963 +/- 473 ng/mL, with a median T1/2 beta for all courses of 22.8 hours (range, 24.1 to 30.6 hours), a Vd of 9.4 L (range, 4.4 to 19.5 L), and a CL of 0.32 L/h (range, 0.12 to 0.55 L/h). Twenty-five percent of the patients on the bolus infusion regimen and 30% on the CI regimen made antibody against mouse Ig (HAMA) and/or ricin A chain antibody (HARA). We conclude that this IT can be administered safely and that both regimens achieve comparable peak serum concentrations at the MTD; these concentrations are similar to those achieved previously using other regimens with IgG-dgA ITs at their respective MTDs. Thus, toxicity is related to the serum level of the IT and does not differ with different targeting MoAbs.
Role of Carnitine Acetyltransferases in Acetyl Coenzyme A Metabolism in Aspergillus nidulans (2011)

The flow of carbon metabolites between cellular compartments is an essential feature of fungal metabolism. During growth on ethanol, acetate, or fatty acids, acetyl units must enter the mitochondrion for metabolism via the tricarboxylic acid cycle, and acetyl coenzyme A (acetyl-CoA) in the cytoplasm is essential for the biosynthetic reactions and for protein acetylation. Acetyl-CoA is produced in the cytoplasm by acetyl-CoA synthetase during growth on acetate and ethanol while β-oxidation of fatty acids generates acetyl-CoA in peroxisomes. The acetyl-carnitine shuttle in which acetyl-CoA is reversibly converted to acetyl-carnitine by carnitine acetyltransferase (CAT) enzymes is important for intracellular transport of acetyl units. In the filamentous ascomycete Aspergillus nidulans, a cytoplasmic CAT, encoded by facC, is essential for growth on sources of cytoplasmic acetyl-CoA while a second CAT, encoded by the acuJ gene, is essential for growth on fatty acids as well as acetate. We have shown that AcuJ contains an N-terminal mitochondrial targeting sequence and a C-terminal peroxisomal targeting sequence (PTS) and is localized to both peroxisomes and mitochondria, independent of the carbon source. Mislocalization of AcuJ to the cytoplasm does not result in loss of growth on acetate but prevents growth on fatty acids. Therefore, while mitochondrial AcuJ is essential for the transfer of acetyl units to mitochondria, peroxisomal localization is required only for transfer from peroxisomes to mitochondria. Peroxisomal AcuJ was not required for the import of acetyl-CoA into peroxisomes for conversion to malate by malate synthase (MLS), and export of acetyl-CoA from peroxisomes to the cytoplasm was found to be independent of FacC when MLS was mislocalized to the cytoplasm.
Discovery of Small-Molecule Inhibitors of the NFAT−Calcineurin Interaction by Competitive High-Throughput Fluorescence Polarization Screening (2004)

The direct protein-protein interaction between the phosphatase calcineurin and transcription factor NFAT plays important roles in a number of crucial mammalian cell signaling and regulatory events, such as activation of T cells and developmental genetic programs. In this paper, we report on the identification of small organic molecules for the targeted disruption of the NFAT-calcineurin interaction in vitro. In the preceding paper (21), we devise a theoretical and procedural framework for high-throughput fluorescence polarization screens to aid in this effort. The results presented here ground on this work and illustrate the stringency and successful general applicability of our approach. The identified compounds provide valuable molecular tools for probing calcineurin signaling and for the NFAT-specific inhibition of calcineurin in cells and organisms.
On Designing Fuzzy Controllers for a Class of Nonlinear Networked Control Systems (2008)

This paper is concerned with controller design for a class of nonlinear networked control systems. These systems are approximated by uncertain linear networked Takagi-Sugeno (T-S) models with both network-induced delay and data packet dropout. Sufficient conditions are derived for the existence of a fuzzy controllers. Then, an iterative algorithm for the controller design is proposed. A control problem of a flexible-joint robot arm system is studied to show the effectiveness of the iterative algorithm.
A 72K CMOS Channelless Gate Array with Embedded 1Mbit Dynamic RAM (1988)

A lMbit DRAM is embedded in 72K raw gates channelless gate array with 1 .Opm HC2MOS twin well technology. The DRAM design is optimized for embedding, such as the adoption of no substrate bias design and p-well protected nchannel memory cells. The typical delay time of the gate array is 0.4ns and the worst case access time of the DRAM is 60ns.
Anti-Amyloid Activity of the C-Terminal Domain of proSP-C against Amyloid β-Peptide and Medin (2009)

Amyloid fibrils are found in approximately 25 different diseases, including Alzheimer's disease. Lung surfactant protein C (SP-C) forms fibrils in association with pulmonary disease. It was recently found that the C-terminal domain of proSP-C (CTC), which is localized to the endoplasmic reticulum (ER) lumen, protects the transmembrane (TM) part of (pro)SP-C from aggregation into amyloid until it has a folded into an alpha-helix. CTC appears to have a more general anti-amyloid effect by also acting on TM regions of other proteins. Here we investigate interactions of CTC with the amyloid beta-peptide (Abeta) associated with Alzheimer's disease and medin, a peptide that forms fibrils in the most common form of human amyloid. CTC prevents fibril formation in Abeta and medin and forms a complex with Abeta oligomers, as judged by size-exclusion chromatography and electrospray ionization mass spectrometry. These data suggest that CTC functions as a chaperone that acts preferentially against unfolded TM segments and structural motifs found during amyloid fibril formation, a mechanism that may be exploited in forming a basis for future anti-amyloid therapy.
Prostaglandins E1 and E2 interact with prostaglandin F2alpha to regulate initiation of DNA replication and cell division in swiss 3T3 cells. (1982)

Prostaglandin (PG) E1 or E2 added at 2-1,000 ng/ml to quiescent cultures of Swiss 3T3 cells synergistically enhanced the rate of initiation of DNA replication stimulated by PGF2 alpha alone or with insulin. Neither PGD2 nor PGF1 alpha had any effect with PGF2 alpha. An increase in the rate of entry into S phase also occurred when PGE1 or PGE2 was added 8 or 15 hr after addition of PGF2 alpha. However, adding PGE1 and PGE2 together with PGF2 alpha did not further enhance the synergistic effect observed with PGE1 or PGE2 separately. The synergistic effect was also observed in stimulation of 2-deoxyglucose uptake but not in early changes of intracellular levels of cAMP. These results may be relevant in understanding the control of fibroblastic proliferation in wound healing and may provide an alternative mechanism for oncogenic transformation.
The Biological chemistry of magnesium (1995)

Fundamentals of inorganic chemistry experimental methods transport and storage metalloproteins and metalloenzymes - oxygen carriers and hydrolases, redox chemistry alkali and alkaline earth metals metals in the regulation of biochemical events cell toxicity and chemotherapeutics metal complexes as probes of structure and reactivity case studies. (Part contents).
Evaluation of trans-9-18F-fluoro-3,4-Methyleneheptadecanoic acid as a PET tracer for myocardial fatty acid imaging. (2005)

The cyclopropyl moiety in (18)F-FCPHA does have a significant influence on heart accumulation, as suggested by the high heart-to-blood ratio and the fast blood clearance in rats. These results, along with the remarkable quality of the PET images, indicate the potential of this new class of labeled fatty acids for use in studying heart disease by PET.
A VLSI architecture for a real-time code book generator and encoder of a vector quantizer (1994)

Image compression applications use vector quantization (VQ) for its high compression ratio and image quality. The current VQ hardware employs static instead of dynamic code book generation as the latter demands intensive computation and corresponding expensive hardware even though it offers better image quality. This paper describes a VLSI architecture for a real-time dynamic code book generator and encoder of 512/spl times/512 images at 30 frames/s. The four-chip 0.8 /spl mu/m CMOS design implements a tree of Kohonen self-organizing maps, and consists of two VQ processors and two image buffer memory chips. The pipelined VQ processor contains a computational core for both code book generation and encoding, and is scalable to processing larger frames.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Stem cells: a problem in asymmetry (1988)

ABSTRACT The special property of stem cells is that their development is asymmetric. They give rise both to cells that are identical to themselves and to cells that are different. The mechanism that provides this asymmetry may be intrinsic or extrinsic. Such mechanisms are considered within the context of other systems where asymmetric development occurs. The specification of mating types in yeast provides a clear example of a stem cell system generated intrinsically. In fission yeast it appears that the asymmetry is due to chromosomal differences: this is the only known mechanism for intrinsic asymmetry. While there is good evidence for intrinsic asymmetry in both plants and invertebrates - particularly the nematode - the mechanism is not known. In insects and vertebrates there is no well established example of intrinsic asymmetry if one excludes asymmetric cytoplasmic localization during cleavage of the egg. Asymmetry is thus due to environmental influences. Stem cell systems are usually well structured and the cell’s behaviour seems to be position-dependent. This is well established for the stem cells of hydra. By contrast it is claimed that the mammalian haemopoietic system is generated by an intrinsic, asymmetric, probabilistic mechanism - the validity of this view is questioned.
<title>Feature management for large video databases</title> (1993)

Large video databases, as well as many other applications involving video, such as multimedia, training, and `movie-on-demand' systems, require efficient steps to manipulate the enormous amount of data associated with full motion video. In this paper the incoming video is systematically and efficiently reduced via a frame selection procedure which takes advantage of the fact that the incoming video is encoded using one of several existing DCT- based standards. The procedure is performed in the frequency domain prior to video decoding. Further refinement in the frame selection step is achieved using a robust metric based upon the color histogram of the selected subset of decoded frames. The procedure is presented in detail and several examples are exhibited.
Characterization of Patterned Self-Assembled Monolayers and Protein Arrays Generated by the Ink-Jet Method (2002)

Commercial ink-jet printers were used with little modification to deposit alkanethiols onto gold substrata and several proteins onto silica supports. The resulting patterns of alkanethiols form self-assembled layers comparable to those obtained by microcontact printing or solution adsorption. The method has been used successfully to create binary chemical gradients and patterns of tertiary functionality. The proteins form dense patterns on the substrates and seem to maintain their configuration as measured by their ability to bind their specific ligands. Four different proteins were printed simultaneously, allowing for positive and negative controls. This “drop-on-demand” printing method is an inexpensive, flexible alternative to current binary technologies of chemically functionalizing surfaces.
Probing the size of proteins with glass nanopores (2014)

Single molecule studies using nanopores have gained attention due to the ability to sense single molecules in aqueous solution without the need to label them. In this study, short DNA molecules and proteins were detected with glass nanopores, whose sensitivity was enhanced by electron reshaping which decreased the nanopore diameter and created geometries with a reduced sensing length. Further, proteins having molecular weights (MW) ranging from 12 kDa to 480 kDa were detected, which showed that their corresponding current peak amplitude changes according to their MW. In the case of the 12 kDa ComEA protein, its DNA-binding properties to an 800 bp long DNA molecule was investigated. Moreover, the influence of the pH on the charge of the protein was demonstrated by showing a change in the translocation direction. This work emphasizes the wide spectrum of detectable molecules using nanopores from glass nanocapillaries, which stand out because of their inexpensive, lithography-free, and rapid manufacturing process.
Development of Single Nucleotide Polymorphism Markers for the Wheat Curl Mite Resistance Gene Cmc4 (2019)

ABSTRACT Wheat curl mite ( Aceria tosichella Keifer) is an important wheat ( Triticum aestivum L. em. Thell.) pest in many wheat‐growing regions worldwide. Mite feeding damage not only directly affects wheat yield, but A. tosichella also transmits Wheat streak mosaic virus (WSMV). Wheat resistance to A. tosichella , therefore, helps control WSMV. OK05312 (PI 670019) is an advanced breeding line released from Oklahoma that shows a high level of A. tosichella resistance. To map the gene(s) conditioning wheat resistance to A. tosichella in OK05312, a genetic linkage map was constructed using single nucleotide polymorphism (SNP) markers derived from genotyping‐by‐sequencing (GBS) and a population of 186 recombinant inbred lines (RILs) from the cross ‘Jerry’ (PI 632433)/OK05312. Seedlings of both parents and the RIL population were infested by A. tosichella Biotype 1 in greenhouse experiments. One major quantitative trait locus was identified on the short arm of chromosome 6D, which corresponds to the previously reported gene Cmc4 for A. tosichella resistance. This gene explained up to 71% of the phenotypic variation and was delimited in a 1.7‐Mb (∼3.3‐cM) region by SNPs 370SNP7523 and 370SNP1639. We successfully converted 12 GBS‐SNPs into Kompetitive allele specific polymerase chain reaction (KASP) markers. Two of them tightly linked to Cmc4 were validated to be highly diagnostic in a US winter wheat population and can be used for marker‐assisted breeding for incorporation of Cmc4 into new wheat cultivars.
Multiwaveforms generator dedicated to selective and continuous stimulations of the bladder (2004)

We present a stimulation system composed of a subcutaneous implantable stimulator and an external controller. It performs selective and continuous stimulations for bladder voiding and its hyperreflexia reduction. A flexible selective stimulation and a measurement technique to monitor the electrodes-nerve contact are proposed. All stimulation parameters are sent wirelessly by an external controller and stored in a RAM. The modular architecture of the design makes the device an expandable multichannel stimulation system. The stimulator has been designed and fabricated in the CMOS 0.18 um technology. It has been tested and its functionality proven.
Oral Administration of Myelin Basic Protein Is Superior to Myelin in Suppressing Established Relapsing Experimental Autoimmune Encephalomyelitis (1999)

Oral administration of a myelin component, myelin basic protein (MBP), induces immunological unresponsiveness to CNS Ags and ameliorates murine relapsing experimental autoimmune encephalomyelitis (REAE). However, a recent clinical trial in which multiple sclerosis patients were treated with repeated doses of oral myelin was unsuccessful in reducing disease exacerbations. Therefore, we directly compared the tolerizing capacity of myelin vs MBP during REAE in B10.PL mice. Oral administration of high doses of myelin, either before disease induction or during REAE, did not provide protection from disease or decrease in vitro T cell responses. In contrast, repeated oral administration of high doses of MBP suppressed established disease and MBP-specific T cell proliferation and cytokine responses. The frequency of IL-2-, IFN-gamma-, and IL-5-secreting MBP-specific T cells declined with MBP feeding, implicating anergy and/or deletion as the mechanism(s) of oral tolerance after high Ag doses. We have previously shown that the dosage and timing of Ag administration are critical parameters in oral tolerance induction. Studies presented here demonstrate that Ag homogeneity is also important, i.e., homogeneous Ag (MBP) is more effective at inducing oral tolerance than heterogeneous Ag (myelin).
Efficient Synthesis of Selectively Protected l-Dopa Derivatives from l-Tyrosine via Reimer−Tiemann and Dakin Reactions (1997)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTEfficient Synthesis of Selectively Protected l-Dopa Derivatives from l-Tyrosine via Reimer−Tiemann and Dakin ReactionsMichael E. Jung and Tsvetelina I. LazarovaView Author Information Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095-1569 Cite this: J. Org. Chem. 1997, 62, 5, 1553–1555Publication Date (Web):March 7, 1997Publication History Received11 November 1996Published online7 March 1997Published inissue 1 March 1997https://pubs.acs.org/doi/10.1021/jo962099rhttps://doi.org/10.1021/jo962099rbrief-reportACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views3623Altmetric-Citations68LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Aldehydes,Aromatic compounds,Oxidation,Oxides,Reaction products Get e-Alerts
Treatment of Refractory/Recurrent C. difficile-associated Disease by Donated Stool Transplanted Via Colonoscopy (2010)

Fecal transplantation via colonoscopy is a safe, effective treatment regimen for refractory/recurrent CDAD. Our 12 patients had an immediate and durable response rate of 100%. Fecal transplantation is a promising treatment for refractory/recurrent C. difficile infection. Its use and efficacy should be pursued in prospective controlled trials.
Humoral defending mechanisms in children of smoking parents. (1987)

In autumn and spring a group of 132 ten-year-old school children (54.5% from families of smokers) were examined for blood content of immunoglobulins (IgG, IgA, (gM, in autumn including also IgE), lysozyme (LYS) and the so called acute reactants (alpha-1-antitrypsin = A1AT; alpha-2-macroglobulin = A2M; transferrin = TRF; ceruloplasmin = CPL); and for saliva sIgA and sLYS. Autumn examination detected significantly higher mean values of IgE in children from families of smokers, while other mean differences remained insignificant. Spring examination revealed significant differences in the means of IgA levels children from families of smokers (FS) had significantly lower levels of IgA while their saliva sIgA values were significantly higher. Mean spring CPL levels in FS were significantly higher. Analysis of distribution curves of autumn examination showed a significant shift of A1AT towards higher values in boys from FS. Girls from FS exhibited a shift of LYS towards lower values. Spring examination in boys FS evidenced a shift of CPL and sIgA values towards higher values; the curve of serum IgA levels split distinctly into two subgroups. In girls from FS the only change observed during the spring examination was a shift of A2M levels towards higher values with an indication of a split. To conclude, passive smoking in school children is responsible for a number of significant changes, the latter being more frequent and marked in spring when the children's organism is weakened by many other unfavourable circumstances. More significant changes were seen in boys.
TREATMENT OF PRURITUS VULVAE BY MULTIPLE INTRADERMAL INJECTIONS OF ALCOHOL. A DOUBLE‐BLIND STUDY (1979)

A double blind study is described in which 17 patients with intractable pruritus vulvae were treated by multiple intradermal injections of absolute alcohol. In each patient half the vulva was treated with alcohol whilst the other half was injected with water. Treatment with alcohol was significantly better than treatment with water (p less than 0.001).