gebhart/patrepeval huggingface dataset:
The natural course of chronic hepatitis C (1994)

In 1988, investigators from the Chiron Company (USA) detected the non-A, non-B agent and named it hepatitis C virus (HCV). An anti-HCV antibody assay (ELISA) and subsequently confirmation tests (immunoblot and polymerase chain reaction) were developed. HCV exposure results in a chronic infection in a majority of cases. This chronic infection is associated with slowly progressive chronic liver disease. Chronic HCV infection is, like HBV, also associated with the development of hepatocellular carcinoma. Most HCV carriers are infected by parenteral routes. Intravenous drug users have the highest risk of becoming infected. Intrafamiliar spread is seen in certain parts of the world but sexual and perinatal transmission does not play an important role in spreading the infection. Antiviral therapy (alpha-interferon) in patients with chronic hepatitis C will normalize liver function tests in about 25% of the cases.
Investigation of dirithromycin and erythromycylamine uptake by human neutrophils in vitro (1994)

Dirithromycin, a new semisynthetic 14-membered-ring macrolide was avidly concentrated by human neutrophils in a time- but not concentration-dependent manner with mean cellular/extracellular, concentration ratios (C/E) of 9 within the first 5 min and up to 47 at 120 min. Erythromycylamine, the hydrolysis product of dirithromycin, was concentrated significantly less by neutrophils, reaching C/E values of 4 and 19 (at 5 and 120 min). A point of interest was the interindividual variability in the antibiotic uptake kinetics; in particular, 7 out of 47 neutrophil samples from different healthy volunteers displayed very slow uptake of both drugs (C/E values at 30 min: dirithromycin, 5.8; erythromycylamine, 4.6). The reason(s) for this is unknown. The uptake of both drugs was decreased at acidic pH and increased at basic pH. Chloroquine, an antimalarial drug which is concentrated in and alkalinizes azurophilic granules, reduced uptake by half. Metabolic inhibitors (2-4 dinitrophenol, sodium fluoride, potassium cyanide and sodium azide) did not impair the uptake of either drug but, interestingly, ouabain, an inhibitor of membrane Na+/K+ ATPase activity, impaired uptake by about 30%. Competitive inhibitors of some transport systems identified on neutrophil membrane (nucleosides, D-glucose and various aminoacids) did not alter the uptake of either drug. Dirithromycin and to a lesser extent, erythromycylamine, reached intracellular concentrations much higher than those required to inhibit the growth of sensitive microorganisms. Although the mechanism of uptake is not clear, one interesting hypothesis involves trapping by protonation into acidic compartments of neutrophils.
Increasing file system availability through second-class replication (2002)

High availability in the Coda file system is achieved through two types of data replication: first-class replication, in which entire servers are replicated, and second-class replication, otherwise known as client caching. The two are complementary: server replication increases the availability of the entire file store, whereas caching permits operation in the event of total disconnection. A particularly valuable use of disconnected operation is the graceful integration of portable computers into the system. The use of second-class replication and its contribution to the system availability and disconnected operation are discussed. Transparent disconnected operation dictates a much-expanded role for client cache managers, effectively turning them into pseudoservers. Three phases of pseudoserver operation-hoarding, server emulation and reintegration-are described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Antibiotic Prophylaxis in Orthopedic Prosthetic Surgery (2001)

Staphylococci account for approximately 70% of postoperative infections in orthopedic prosthetic surgery, with the leading organism being Staphylococcus epidermidis. Therefore, the antibiotics most widely used for prophylaxis are cefazolin, cefamandole and cefuroxime, by virtue of their excellent activity against these pathogens. However, methicillin-resistant coagulase-positive and -negative staphylococci are increasingly being reported as the causative agents of postoperative infection in clean prosthetic surgery, therefore prompting the use of glycopeptide antibiotics (vancomycin and teicoplanin) in the prophylaxis for total joint replacement, particularly in hospitals in which there is high methicillin-resistance among these pathogens. Furthermore, in total primary knee replacement (TKR) performed with tourniquet application, regional prophylaxis with teicoplanin (i.e. the injection of teicoplanin into a foot vein of the leg to be operated on after occlusion of the systemic circulation by inflating the tourniquet) has been shown to be a safe and valuable prophylactic technique. Apart from providing teicoplanin concentrations in the operative field higher than those achievable by conventional systemic prophylaxis (i.e. by injecting the antibiotic into an arm vein before application of the tourniquet), regional prophylaxis was found in a prospective, controlled, open study on patients undergoing primary monolateral or bilateral TKR to result in a rate of postoperative infection similar to those achievable with conventional prophylactic regimens.
Synthesis of sugar-substituted cyclic and polymeric phosphazenes and their oxidation, reduction, and acetylation reactions (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of sugar-substituted cyclic and polymeric phosphazenes and their oxidation, reduction, and acetylation reactionsHarry R. Allcock and Angelo G. ScopelianosCite this: Macromolecules 1983, 16, 5, 715–719Publication Date (Print):May 1, 1983Publication History Published online1 May 2002Published inissue 1 May 1983https://pubs.acs.org/doi/10.1021/ma00239a001https://doi.org/10.1021/ma00239a001research-articleACS PublicationsRequest reuse permissionsArticle Views283Altmetric-Citations62LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Emtricitabine Prodrugs with Improved Anti-HIV Activity and Cellular Uptake (2012)

Three fatty acyl conjugates of (-)-2',3'-dideoxy-5-fluoro-3'-thiacytidine (FTC, emtricitabine) were synthesized and evaluated against HIV-1 cell-free and cell-associated virus and compared with the corresponding parent nucleoside and physical mixtures of FTC and fatty acids. Among all the compounds, the myristoylated conjugate of FTC (5, EC(50) = 0.07-3.7 μM) displayed the highest potency. Compound 5 exhibited 10-24 and 3-13-times higher anti-HIV activity than FTC alone (EC(50) = 0.7-88.6 μM) and the corresponding physical mixtures of FTC and myristic acid (14, EC(50) = 0.2-20 μM), respectively. Cellular uptake studies confirmed that compound 5 accumulated intracellularly after 1 h of incubation and underwent intracellular hydrolysis in CCRF-CEM cells. Alternative studies were conducted using the carboxyfluorescein conjugated with FTC though β-alanine (12) and 12-aminododecanoic acid (13). Acylation of FTC with a long-chain fatty acid in 13 improved its cellular uptake by 8.5-20 fold in comparison to 12 with a short-chain β-alanine. Compound 5 (IC(90) = 15.7-16.1 nM) showed 6.6- and 35.2 times higher activity than FTC (IC(90) = 103-567 nM) against multidrug resistant viruses B-NNRTI and B-K65R, indicating that FTC conjugation with myristic acid generates a more potent analogue with a better resistance profile than its parent compound.
Effective distributed scheduling of parallel workloads (1996)

We present a distributed algorithm for time-sharing parallel workloads that is competitive with coscheduling. Implicit scheduling allows each local scheduler in the system to make independent decisions that dynamically coordinate the scheduling of cooperating processes across processors. Of particular importance is the blocking algorithm which decides the action of a process waiting for a communication or synchronization event to complete. Through simulation of bulk-synchronous parallel applications, we find that a simple two-phase fixed-spin blocking algorithm performs well; a two-phase adaptive algorithm that gathers run-time data on barrier wait-times performs slightly better. Our results hold for a range of machine parameters and parallel program characteristics. These findings are in direct contrast to the literature that states explicit coscheduling is necessary for fine-grained programs. We show that the choice of the local scheduler is crucial, with a priority-based scheduler performing two to three times better than a round-robin scheduler. Overall, we find that the performance of implicit scheduling is near that of coscheduling (+/- 35%), without the requirement of explicit, global coordination.
Reactivity Assays of Surface Hydroxyl Chain Ends of Poly(ethylene terephthalate) (PET) Film and Membranes Using Original3H- and Fluorine-Labeled Derivatization Reagents (1996)

The hydroxyl chain ends (PET-OH) displayed on the surface of poly(ethylene terephthalate) film and track-etched membranes of two different porosities were assayed by derivatization with reagents containing 3H and fluorine tags. After activation by tosylation, the resulting sulfonate esters (PET-OTs) were substituted with l-N-(trifluoroethyl)[4,5-3H]lysinamide. On the other hand, direct coupling of PET-OH with heptafluorobutyl (p-isocyanatobenzoyl)[2-3H]glycinate was performed; subsequent treatement with l-[4,5-3H]lysine partially gave the ester substitution. All the samples were analyzed by liquid scintillation counting (LSC) and X-ray photoelectron spectroscopy (XPS). The ratios of derivatization recorded by LSC measurements, for membranes currently used as substrates in cell cultivation, were within 15−30 pmol/cm2 of fixed amino acid labels. From XPS, we determined that 0.5−1% of the polymer units were derivatized in about 50 Å depth. This study establishes practical conditions for the covalent anchorage of biologically active molecules on PET samples.
A self‐assembled antibody electrode for HT29 tumour cells (1997)

The present paper describes a self-assembled monoclonal-antibody immunosensor for HT29 tumour cells. This immunosensor is composed of two parts. One is a three-electrode system and the other is a layer of anti-(human colonic tumour) monoclonal antibody DF-I, which self-assembled on to the gold electrode. Two kinds of cells, HT29 tumour cells and normal fibroblast cells, were compared using the antibody electrode. The result was the same as that obtained by using immune luminescence. The HT29 cells can be captured specifically by the assembled anti-HT29 monoclonal-antibody electrode and can be measured by an electrochemical method.
Idiopathische hypercalcemie bij een Perzische kat (2013)

In deze casuïstiek wordt een kat met idiopathische hypercalcemie beschreven. Een mannelijke,gecastreerde Perzische kat van zeven jaar werd aangeboden omwille van partiële anorexie die reedsdrie jaar aanwezig was. Op het bloedonderzoek werd een matige hypercalcemie in combinatiemet hypofosfatemie gezien. Door middel van uitgebreide diagnostiek werd vastgesteld dat de kat‘polycystic kidney disease’ en chronische nierziekte (IRIS fase 2) had. De nierziekte lag echter nietaan de basis van de hypercalcemie gezien de concentratie parathyroïd hormoon normaal was ende kat hypofosfatemie en een gestegen geïoniseerde calciumconcentratie vertoonde. De diagnosevan idiopathische hypercalcemie werd gesteld. De symptomen verdwenen en de calciumspiegelnormaliseerde na behandeling met glucocorticoïden.
Serine‐202 is the putative precursor of the active site dehydroalanine of phenylalanine ammonia lyase Site‐directed mutagenesis studies on the enzyme from parsley (Petroselinum crispum L.) (1994)

To investigate the possible role of serine as a precursor of dehydroalanine at the active site of phenylalanine ammonia lyase, two serines, conserved in all known PAL and histidase sequences, were changed to alanine by site-directed mutagenesis. The resulting mutant genes were subcloned into the expression vector pT7.7 and the gene products were assayed for PAL activity. Mutant PALMutS209A showed the same catalytic property as wild-type PAL, whereas mutant PALMutS202A was devoid of catalytic activity, indicating that serine-202 is the most likely precursor of the active site dehydroalanine.
Spatiotemporal modulation for mid-air haptic feedback from an ultrasonic phased array (2018)

&amp;lt;!--StartFragment--&amp;gt;A tactile sensation can be experienced by focusing airborne ultrasound using a phased array. Nonlinear acoustic pressure alone is difficult to perceive so traditional methods use amplitude modulation in the range of 10–300 Hz to stimulate nerves on the hand most sensitive to those frequencies. We demonstrate that through rapid translation of focus points similar results can be obtained using spatiotemporal modulation. This allows for volumetric sensations to be created using maximum power possible from the array. This is made possible through a solving approach using a pseudo-inverse of the activation matrix followed by a power iteration eigenvalue solution. Advantages versus amplitude modulation and consequences for parametric audio will be discussed.&amp;lt;!--EndFragment--&amp;gt;
Scaleable chromatographic purification process for recombinant adeno-associated virus (rAAV) (2000)

This study demonstrates the feasibility of using column chromatography alone for the isolation of highly purified rAAV vector. The methods described here are advancements in procedures to purify rAAV and are adaptable for commercial production of clinical-grade rAAV vector.
Change in osmolarity of disodium cromoglycate solution and protection against exercise-induced bronchospasm in children with asthma (1996)

It has been suggested that osmolarity and/or nebulizer output may affect the protective effects of disodium cromoglycate (DSCG) in asthma. The aim of this study was to evaluate the influence of osmolarity of the DSCG solution on exercise-induced bronchospasm (EIB) in children with bronchial asthma. A jet nebulizer was used for DSCG inhalation in Study 1 and an ultrasonic nebulizer in Study 2. Thirteen asthmatic children (7 males and 6 females, aged 6-14 yrs) were enrolled in Study 1, and nine asthmatic children (5 males and 4 females, aged 9-13 yrs) in Study 2. After pretreatment with saline (control), hypotonic DSCG or isotonic DSCG, children underwent exercise challenge with a cycle ergometer. The percentage fall in forced expiratory volume in one second (FEV1) was measured at 5 and 15 min postexercise. The data were compared by analysis of variance (ANOVA). Both in Study 1 and Study 2, there were no significant differences in minute ventilation volume or maximum heart rate during exercise between the different treatment groups. Both hypotonic and isotonic DSCG significantly reduced the maximum percentage fall in FEV1. There were no significant differences in protective effects between hypotonic and isotonic DSCG in either study. We conclude that the efficacy of hypotonic and isotonic disodium cromoglycate solutions is similar for protection against exercise-induced bronchospasm. Hypotonic disodium cromoglycate seems to be clinically effective for prevention of exercise-induced bronchospasm and treatment of asthmatic children.
c-maf Promotes T Helper Cell Type 2 (Th2) and Attenuates Th1 Differentiation by Both Interleukin 4–dependent and –independent Mechanisms (1998)

The c-maf protooncogene is a T helper cell type 2 (Th2)-specific transcription factor that activates the interleukin (IL)-4 promoter in vitro. Although it has been postulated that c-maf directs the Th2-specific expression of the IL-4 gene in vivo, direct evidence that c-maf functions during the differentiation of normal, primary T cells is lacking. We now demonstrate that overexpression of c-maf in vivo skews the Th immune response along a Th2 pathway, as evidenced by increased production of Th2 cytokines and the IL-4-dependent immunoglobulins, IgG1 and IgE. The overproduction of IgGl and IgE in the CD4 promoter/c-maf transgenic mice was IL-4 dependent since this was not observed in c-maf transgenic mice bred onto an IL-4-deficient background. Ectopic expression of c-maf in mature Th1 cells did not confer on them the ability to produce IL-4, but did decrease the production of IFN-gamma. The attenuation of Th1 differentiation by c-maf overexpression occurred by a mechanism that was independent of IL-4 and other Th2 cytokines, and could be overcome by IL-12. These studies demonstrate that c-maf promotes Th2 differentiation by IL-4-dependent mechanisms and attenuates Th1 differentiation by Th2 cytokine-independent mechanisms.
Induction of a Mucosal Cytotoxic T-Lymphocyte Response by Intrarectal Immunization with a Replication-Deficient Recombinant Vaccinia Virus Expressing Human Immunodeficiency Virus 89.6 Envelope Protein (1998)

To improve the safety of recombinant vaccinia virus vaccines, modified vaccinia virus Ankara (MVA) has been employed, because it has a replication defect in most mammalian cells. Here we apply MVA to human immunodeficiency virus type 1 (HIV-1) vaccine development by incorporating the envelope protein gp160 of HIV-1 primary isolate strain 89.6 (MVA 89.6) and use it to induce mucosal cytotoxic-T-lymphocyte (CTL) immunity. In initial studies to define a dominant CTL epitope for HIV-1 89.6 gp160, we mapped the epitope to a sequence, IGPGRAFYAR (from the V3 loop), homologous to that recognized by HIV MN loop-specific CTL and showed that HIV-1 MN-specific CTLs cross-reactively recognize the corresponding epitope from strain 89.6 presented by H-2Dd. Having defined the CTL specificity, we immunized BALB/c mice intrarectally with recombinant MVA 89.6. A single mucosal immunization with MVA 89.6 was able to elicit long-lasting antigen-specific mucosal (Peyer's patch and lamina propria) and systemic (spleen) CTL responses as effective as or more effective than those of a replication-competent vaccinia virus expressing 89.6 gp160. Immunization with MVA 89.6 led to (i) the loading of antigen-presenting cells in vivo, as measured by the ex vivo active presentation of the P18-89.6 peptide to an antigen-specific CTL line, and (ii) the significant production of the proinflammatory cytokines (interleukin-6 and tumor necrosis factor alpha) in the mucosal sites. These results indicate that nonreplicating recombinant MVA may be at least as effective for mucosal immunization as replicating recombinant vaccinia virus.
Nonparametric information fusion for motion estimation (2003)

The problem of information fusion appears in many forms in vision. Tasks such as motion estimation, multimodal registration, tracking, and robot localization, often require the synergy of estimates coming from multiple sources. Most of the fusion algorithms, however, assume a single source model and are not robust to outliers. If the data to be fused follow different underlying models, the traditional algorithms would produce poor estimates. We present in this paper a nonparametric approach to information fusion called variable-bandwidth density-based fusion (VBDF). The fusion estimator is computed as the location of the most significant mode of a density function, which takes into account the uncertainty of the estimates to be fused. A mode detection scheme is presented, which relies on variable-bandwidth mean shift computed at multiple scales. We show that the proposed estimator is consistent and conservative, while handling naturally outliers in the data and multiple source models. The new theory is tested for the task of multiple motion estimation. Numerous experiments validate the theory and provide very competitive results.
Mass spectrometic detection for capillary isoelectric focusing separations of complex protein mixtures (2000)

Capillary isoelectric focusing (CIEF) can provide high-resolution separations of complex protein mixtures, but until recently it has primarily been used with conventional UV detection. This technique would be greatly enhanced by much more information-rich detection methods that can aid in protein characterization. We describe progress in the development of the combination of CIEF with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry and its application to proteome characterization. Studies have revealed 400-1000 putative proteins in the mass range of 2-100 kDa from total injections of approximately 300 ng protein in single CIEF-FTICR analyses of cell lysates for both Escherichia coli (E. coli) and Deinococcus radiodurans (D. radiodurans). We also demonstrate the use of isotope labeling of the cell growth media to improve mass measurement accuracy and provide a means for quantitative proteome-wide measurements of protein expression. The ability to make such comprehensive and precise measurements of differences in protein expression in response to cellular perturbations should provide new insights into complex cellular processes.
Voxel‐Based Morphometry Reveals Gray Matter Abnormalities in Migraine (2007)

Our study shows that migraine is associated with a significant gray matter reduction in several of the cortical areas involved in pain circuitry. In addition, we found a significant correlation between frequency of migraine attacks and signal alteration in the Anterior Cingulate Cortex. Our data provide new insight into migraine pathophysiology and support the concept that migraine may be a progressive disorder.
Neural Optimizer Search with Reinforcement Learning (2017)

We present an approach to automate the process of discovering optimization methods, with a focus on deep learning architectures. We train a Recurrent Neural Network controller to generate a string in a domain specific language that describes a mathematical update equation based on a list of primitive functions, such as the gradient, running average of the gradient, etc. The controller is trained with Reinforcement Learning to maximize the performance of a model after a few epochs. On CIFAR-10, our method discovers several update rules that are better than many commonly used optimizers, such as Adam, RMSProp, or SGD with and without Momentum on a ConvNet model. We introduce two new optimizers, named PowerSign and AddSign, which we show transfer well and improve training on a variety of different tasks and architectures, including ImageNet classification and Google's neural machine translation system.