gebhart/patrepeval huggingface dataset:
A novel integrin specificity exemplified by binding of the alpha v beta 5 integrin to the basic domain of the HIV Tat protein and vitronectin. (1993)

Several studies have addressed the interaction of the HIV Tat protein with the cell surface. Our analysis of the cell attachment-promoting activity of Tat and peptides derived from it revealed that the basic domain of Tat, not the arg-gly-asp (RGD) sequence, is required for cell attachment to Tat. Affinity chromatography with Tat peptides and immunoprecipitation with various anti-integrin antibodies suggest that the vitronectin-binding integrin, alpha v beta 5, is the cell surface protein that binds to the basic domain of Tat. The Tat basic domain contains the sequence RKKRRQRRR. A related sequence, KKQRFRHRNRKG, present in the heparin-binding domain of an alpha v beta 5 ligand, vitronectin, also bound alpha v beta 5 in affinity chromatography and, in combination with an RGD peptide, was an inhibitor of cell attachment to vitronectin. The alpha v beta 5 interaction with these peptides was not solely due to high content of basic amino acids in the ligand sequences; alpha v beta 5 did not bind substantially to peptides consisting entirely of arginine or lysine, whereas a beta 1 integrin did bind to these peptides. The interaction of alpha v beta 5 with Tat is atypical for integrins in that the binding to Tat is divalent cation independent, whereas the binding of the same integrin to an RGD-containing peptide or to vitronectin requires divalent cations. These data define an auxiliary integrin binding specificity for basic amino acid sequences. These basic domain binding sites may function synergistically with the binding sites that recognize RGD or equivalent sequences.
Gate Injection Transistor (GIT)—A Normally-Off AlGaN/GaN Power Transistor Using Conductivity Modulation (2007)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> We have developed a normally-off GaN-based transistor using conductivity modulation, which we call a gate injection transistor (GIT). This new device principle utilizes hole-injection from the p-AlGaN to the AlGaN/GaN heterojunction, which simultaneously increases the electron density in the channel, resulting in a dramatic increase of the drain current owing to the conductivity modulation. The fabricated GIT exhibits a threshold voltage of 1.0 V with a maximum drain current of 200 mA/mm, in which a forward gate voltage of up to 6 V can be applied. The obtained specific <emphasis emphasistype="smcaps">ON</emphasis>-state resistance (<formula formulatype="inline"><tex>$R_{ \scriptstyle{\rm ON} } \cdot A$</tex></formula>) and the <emphasis emphasistype="smcaps">OFF</emphasis>-state breakdown voltage (<formula formulatype="inline"><tex>$\hbox{BV}_{\rm ds}$</tex></formula>) are 2.6 <formula formulatype="inline"><tex>$\hbox{m}\Omega\cdot \hbox{cm}^{2}$</tex></formula> and 800 V, respectively. The developed GIT is advantageous for power switching applications. </para>
Heteromeric and Homomeric Transforming Growth Factor-β Receptors Show Distinct Signaling and Endocytic Responses in Epithelial Cells (1998)

Transforming growth factor-beta (TGF-beta) induces distinct responses dependent upon the cellular context. It is unclear whether the initial receptor interactions identified in one cell type will be operative in another. Utilizing a chimeric receptor strategy we have examined the signaling and endocytic activity of both heteromeric (type I/type II) and homomeric (type I/type I or type II/type II) TGF-betaR interactions in Mv1Lu epithelial cells. In agreement with that observed in mesenchymal cells, all TGF-betaR signaling in Mv1Lu cells required the formation of a heteromeric type I-type II receptor complex. However, the initial endocytic response to TGF-betaR oligomerization was distinctly regulated in the two cell types. While heteromeric TGF-beta receptors were internalized and down-regulated, homomeric TGF-betaR interactions showed diminished endocytic activity in Mv1Lu cells. This contrasts to that observed in mesenchymal cultures where ligand bound to TGF-betaR homomers was internalized, yet the receptors were not down-regulated. Moreover, while previous reports have suggested that mutations at serine 172 or threonine 176 in the type I TGF-betaR separated transcriptional from proliferative responses, we found no separation of pathways or effect on initial endocytic activity when the analogous mutations were made in the chimeric receptors.
Design of Novel Antibiotics that Bind to the Ribosomal Acyltransfer Site (2002)

The structure of neamine bound to the A site of the bacterial ribosomal RNA was used in the design of novel aminoglycosides. The design took into account stereo and electronic contributions to interactions between RNA and aminoglycosides, as well as a random search of 273 000 compounds from the Cambridge structural database and the National Cancer Institute 3-D database that would fit in the ribosomal aminoglycoside-binding pocket. A total of seven compounds were designed and subsequently synthesized, with the expectation that they would bind to the A-site RNA. Indeed, all synthetic compounds were found to bind to the target RNA comparably to the parent antibiotic neamine, with dissociation constants in the lower micromolar range. The synthetic compounds were evaluated for antibacterial activity against a set of important pathogenic bacteria. These designer antibiotics showed considerably enhanced antibacterial activities against these pathogens, including organisms that hyperexpressed resistance enzymes to aminoglycosides. Furthermore, analyses of four of the synthetic compounds with two important purified resistance enzymes for aminoglycosides indicated that the compounds were very poor substrates; hence the activity of these synthetic antibiotics does not appear to be compromised by the existing resistance mechanisms, as supported by both in vivo and in vitro experiments. The design principles disclosed herein hold the promise of the generation of a large series of designer antibiotics uncompromised by the existing mechanisms of resistance.
Anterior subluxation of the cervical spine: hyperflexion sprain. (1981)

Anterior subluxation (hyperflexion sprain) is localized, purely ligamentous disruption of the cervical spine caused by a limited flexion force. When associated with a simple wedge fraction, also a flexion injury, anterior subluxation may be the more significant lesion. Radiographically, anterior subluxation is characterised by (1) a localized kyphotic angulation at the level of injury; (2) anterior rotation, or displacement, of the subluxed vertebra; (3) anterior narrowing and posterior widening of the disc space; (4) widening of the space between the subluxed vertebral body and the subjacent articular masses; (5) displacement of the inferior articulating facets of the subluxed vertebra with respect to their contiguous subjacent facets; and (6) widening of the interspinous space ("fanning"). The localized kyphotic angulation at the level of ligamentous disruption distinguishes pathologic anterior subluxation from diffuse "reversal of the normal cervical lordosis"; produced by voluntary positioning or muscle spasm. Anterior subluxation is clinically significant because of the approximate 20% incidence of delayed instability due to impaired ligamentous healing.
Dual band switched-parasitic wire antennas for communications and direction finding (2002)

Wire dipole and monopole switched-parasitic antennas have been designed to operate at 900 and 1900 MHz simultaneously. The array consists of a centre driven element circled by four parasitic elements symmetrically arranged. DC levels applied to p.i.n. diodes on each parasitic element allow full 360 degree coverage in the horizontal plane. The design of the antenna was undertaken using the genetic algorithm technique with the NEC2 solver. The design was optimised for element spacing and lumped impedance size and position. The cost function included the input impedance and directional characteristics at both frequencies. The final design has a front to back ratio of approximately 15 dB, S/sub 11/ less than -35 dB and 360 degree coverage of within 1.5 dB of the main lobe gain for both frequencies.
Mass Spectrometry-Based Proteolytic Mapping for Rapid Virus Identification (2002)

A novel method is proposed for rapid identification of viruses and other organisms that show a low number of biomarkers, based on the construction of databases of organism-specific tryptic peptide masses. The peptide products of any protease that cuts at specific residues can be accommodated. Experimentally, a sample of intact virus, e.g., one collected from the atmosphere, is digested with a selective protease for a short time, and the digestion products are analyzed by MALDI-TOF mass spectrometry without fractionation or purification. In the present proof of concept, the Sindbis virus AR 339 was identified by using the masses of observed tryptic peptide products to query a database composed of tryptic peptide masses generated in silico for six viruses whose genomes have been sequenced. Two algorithms were tested for identification--a direct score-ranking algorithm and an algorithm that evaluates the probability of random matching. The Sindbis virus was unambiguously identified by either approach. The influence of factors such as experimental mass accuracy, number of missed cleavages, and database size on the identification algorithms has also been evaluated, with the objective of extending the approach to other microorganisms.
Advanced multiplexed analysis with the FlowMetrixTM system (1997)

Abstract The FlowMetrixTM System is a multiplexed data acquisition and analysis platform for flow cytometric analysis of microsphere-based assays that performs simultaneous measurement of up to 64 different analytes. The system consists of 64 distinct sets of fluorescent microspheres and a standard benchtop flow cytometer interfaced with a personal computer containing a digital signal processing board and Windows95®-based software. Individual sets of microspheres can be modified with reactive components such as antigens, antibodies, or oligonucleotides, and then mixed to form a multiplexed assay set. The digital signal-processing hardware and Windows95-based software provide complete control of the flow cytometer and perform real-time data processing, allowing multiple independent reactions to be analyzed simultaneously. The system has been used to perform qualitative and quantitative immunoassays for multiple serum proteins in both capture and competitive inhibition assay formats. The system has also been used to perform DNA sequence analysis by multiplexed competitive hybridization with 16 different sequence-specific oligonucleotide probes.
Biomechanical pulping of loblolly pine with different strains of the white-rot fungus Ceriporiopsis subvermispora (1992)

Des copeaux de P.taeda ont ete traites par cinq souches de Ceriporiopsis subvermispora pendant quatre semaines avant mise en pâte mecanique sur raffineur. Les pertes de poids des copeaux lors du traitement fongique ont ete de 4 a 7%. La consommation d'energie lors du defibrage et du raffinage a diminue de 21 a 37%. Les resistances a l'eclatement et au dechirement ont ete ameliorees respectivement de 33-46% et de 47-60%. La resistance a la traction n'a pas ete modifiee, de meme que l'opacite. La blancheur et le coefficient de diffusion de la lumiere ont ete diminues.
Posthypotensive generation of superoxide free radicals--possible role in the pathogenesis of the intestinal mucosal damage. (1984)

Graded small intestinal ischemia was induced in cats by partial occlusion of the superior mesenteric artery. Specimens from the small intestine were studied at the end of the hypotensive period, 10 min and 60 min posthypotensively . Specimens obtained late during hypotension revealed characteristic mucosal damage in all cases. However, the intestinal mucosal damage was more pronounced in the specimens taken posthypotensively . This aggravation was prevented if the cats were given superoxide dismutase (SOD) during the hypotensive period. Further, the intestinal tissue concentration of total glutathione was reduced posthypotensively . It is suggested that the posthypotensive aggravation of the intestinal mucosal damage is caused by generation of cytotoxic oxygen free radicals. Data favouring the suggestion that the glutathione-glutathione peroxidase system is an important generator of free radicals in intestinal ischemia is presented.
Metabolism of d -Arabinose: Origin of a d -Ribulokinase Activity in Escherichia coli (1971)

The kinase responsible for the phosphorylation of d-ribulose was purified 45.5-fold from a strain of Escherichia coli K-12 capable of growth on d-arabinose with no separation of d-ribulo- or l-fuculokinase activities. Throughout the purification, the ratios of activities remained essentially constant. A nonadditive effect of combining both substrates in an assay mixture; identical K(m) values for adenosine triphosphate with either l-fuculose or d-ribulose as substrate; and, the irreversible loss of activity on both substrates, after removal of magnesium ions from the enzyme preparation, suggest that the dual activity is due to the same enzyme. A fourfold greater affinity of the enzyme for l-fuculose than for d-ribulose, as well as a higher relative activity on l-fuculose, suggest that the natural substrate for this enzyme is l-fuculose. The product of the purified enzyme, with d-ribulose as substrate, was prepared. The ratio of total phosphorous to ribulose phosphate was 1.01:1, indicating that the product was ribulose monophosphate. The behavior of the kinase product in the cysteine-carbazole and orcinol reactions, as well as the results of periodate oxidation assays, provided evidence that it was not d-ribulose-5-phosphate. Reaction of this compound with a cell-free extract of E. coli possessing l-fuculose-l-phosphate aldolase activity resulted in the production of dihydroxyacetone phosphate and glycolaldehyde. The kinase product failed to reduce 2,3,5-triphenyltetrazolium and possessed a half-life of approximately 1.5 min in the presence of 1 n HCl at 100 C. These properties suggested that the phosphate group was attached to carbon atom 1 of d-ribulose.
Non-invasive Continuous Blood Pressure Monitoring Based on PWTT (2011)

Most non-invasive continuous blood pressure measurements are based on oscillometric methods that use electronic pump, oscillometric electronic sensor and computer aided analysis to display blood pressure. This device is inconvenience for patience due to prolong pumping activities. Several studies have shown that regression model, a function of pulse wave transit time (PWTT), is adequate to estimate systolic blood pressure (SBP). However, the model is unique for each person hence not suitable for general use for continuous measurement of BP. This paper proposed a new generalized regression model that is based on average slopes of those regression models. It has been shown that the proposed model is not unique to a person and adequate to estimate the SBP for various subjects. Thus, the proposed model may be used for non-invasive continuous SBP monitoring.
[Interferon alpha in the treatment of non-Hodgkin's lymphomas of low malignancy]. (1998)

Incidence of non Hodgkin's lymphomas (NHL) has been increased regularly during the last two decades. Overall survival did not progress at all during this period. According to the results of preliminary studies, alpha interferon is an attractive approach for NHL treatment. The review analyze published randomized controlled trials which tested interferon alpha either in addition with polychemotherapy or as maintenance of chemotherapy-induced response in disseminated low grade NHL. After literature search, nine studies have been included. Interpretation of results was complicated by various patient's selection criteria (age, tumoral burden, histology) and heterogeneous treatment schemes (interferon schedule and dose, chemotherapy combination). Significant overall improvement was observed in two studies while only relapse free survival and time to treatment failure were improved in seven trials, always in interferon group. Significant observed toxicities were hematologic ones and asthenia since they led either to dose adjustment or to interferon interruption. Finally, we cannot recommend interferon use out of prospective trials. Further studies are warranted to confirm overall survival benefit and to define optimal strategy to use this molecule.
Novel technologies for the detection and mitigation of drowsy driving (2014)

In the human control of motor vehicles, there are situations regularly encountered wherein the vehicle operator becomes drowsy and fatigued due to the influence of long work days, long driving hours, or low amounts of sleep. Although various methods are currently proposed to detect drowsiness in the operator, they are either obtrusive, expensive, or otherwise impractical. The method of drowsy driving detection through the collection of Steering Wheel Movement (SWM) signals has become an important measure as it lends itself to accurate, effective, and cost-effective drowsiness detection. In this dissertation, novel technologies for drowsiness detection using Inertial Measurement Units (IMUs) are investigated and described. IMUs are an umbrella group of kinetic sensors (including accelerometers and gyroscopes) which transduce physical motions into data. Driving performances were recorded using IMUs as the primary sensors, and the resulting data were used by artificial intelligence algorithms, specifically Support Vector Machines (SVMs) to determine whether or not the individual was still fit to operate a motor vehicle. Results demonstrated high accuracy of the method in classifying drowsiness. It was also shown that the use of a smartphone-based approach to IMU monitoring of drowsiness will result in the initiation of feedback mechanisms upon a positive detection of drowsiness. These feedback mechanisms are intended to notify the driver of their drowsy state, and to dissuade further driving which could lead to crashes and/or fatalities. The novel methods not only demonstrated the ability to qualitatively determine a drivers drowsy state, but they were also low-cost, easy to implement, and unobtrusive to drivers. The efficacy, ease of use, and ease of access to these methods could potentially eliminate many barriers to the implementation of the technologies. Ultimately, it is hoped that these findings will help enhance traveler safety and prevent deaths and injuries to users.
Distributed output encoding for multi-class pattern recognition (2003)

Fingerspelling recognition and handshape recognition are two examples of real-world, multi-class recognition problems consisting of 26 and 78 classes respectively. While it is theoretically possible to solve any multi-class problem with a single "smart" classifier the complexity of such a classifier is usually prohibitively high. This paper looks at several approaches to solving a numerous multi-class recognition problem and discusses in detail a method involving coded output. Experiments are conducted using biomechanical data from a human hand as input, but work is continuing concerning the extraction of this data from multi-view hand images alone. Code generation is discussed and results are presented for several different coded output cases including the Hamming, Golay, and several hybrid codes. Conclusions show that the recognition accuracy increases proportionally to code length.
Microfluidic laser embedded in glass by three-dimensional femtosecond laser microprocessing (2004)

Microfluidic dye lasers three-dimensionally embedded in glass have been fabricated for what is believed to be the first time by integrating micro-optical and microfluidic components by use of a femtosecond laser. By pumping the microfluidic laser, in which the microfluidic chamber was filled with the laser dye Rhodamine 6G dissolved in ethanol, with a frequency-doubled Nd:yttrium aluminum garnet laser, lasing action was confirmed by analysis of the emission spectra at different pump powers. In addition, by arranging two microfluidic chambers serially in the glass, we built a microfluidic twin laser that produces an array of two simultaneous laser emissions with one pump laser.
Thalidomide Metabolites and Analogues. 3. Synthesis and Antiangiogenic Activity of the Teratogenic and TNFα-Modulatory Thalidomide Analogue 2-(2,6-Dioxopiperidine-3-yl)phthalimidine (2003)

Versatile synthesis of the teratogenic, TNFalpha-modulatory, and antiangiogenic thalidomide analogue 2-(2,6-dioxopiperidine-3-yl)phthalimidine (1) and its direct antiangiogenic properties are described. With thalidomide or thalidomide derivatives as precursors, the synthesis involved either carbonyl reduction/thiation-desulfurization or carbonyl reduction/acyliminium ion reduction protocols. Compared to earlier studies with thalidomide, which was only active with microsomal treatment, 1 exhibited marginal inhibitory activity in the rat aortic ring assay, thereby demonstrating the requirement for metabolic activation.
Characterization and proposed nomenclature of epidemic strains of methicillin-resistant Staphylococcus aureus in Canada. (1999)

We hope that standardized nomenclature for identifying epidemic MRSA strains prevalent in Canadian hospitals will be helpful to physicians and infection control practitioners attempting to understand and control the spread of the organism in health-care facilities. It is anticipated that as MRSA continues to evolve in Canadian health-care facilities other strains may be recognized as "epidemic"; as these strains become better characterized they may be added to those designated above. Laboratory physicians and infection control personnel are invited to submit strains that may warrant characterization and designation as a Canadian epidemic strain to the Laboratory Centre for Disease Control, Health Canada, Winnipeg, Manitoba.
Metabolism of Fructooligosaccharides by Lactobacillus paracasei 1195 (2003)

Fermentation of fructooligosaccharides (FOS) and other oligosaccharides has been suggested to be an important property for the selection of bacterial strains used as probiotics. However, little information is available on FOS transport and metabolism by lactic acid bacteria and other probiotic bacteria. The objectives of this research were to identify and characterize the FOS transport system of Lactobacillus paracasei 1195. Radiolabeled FOS was synthesized enzymatically from [(3)H]sucrose and purified by column and thin-layer chromatography, yielding three main products: glucose (G) alpha-1,2 linked to two, three, or four fructose (F) units (GF(2), GF(3), and GF(4), respectively). FOS hydrolysis activity was detected only in cell extracts prepared from FOS- or sucrose-grown cells and was absent in cell supernatants, indicating that transport must precede hydrolysis. FOS transport assays revealed that the uptake of GF(2) and GF(3) was rapid, whereas little GF(4) uptake occurred. Competition experiments showed that glucose, fructose, and sucrose reduced FOS uptake but that other mono-, di-, and trisaccharides were less inhibitory. When cells were treated with sodium fluoride, iodoacetic acid, or other metabolic inhibitors, FOS transport rates were reduced by up to 60%; however, ionophores that abolished the proton motive force only slightly decreased FOS transport. In contrast, uptake was inhibited by ortho-vanadate, an inhibitor of ATP-binding cassette transport systems. De-energized cells had low intracellular ATP concentrations and had a reduced capacity to accumulate FOS. These results suggest that FOS transport in L. paracasei 1195 is mediated by an ATP-dependent transport system having specificity for a narrow range of substrates.