gebhart/patrepeval huggingface dataset:
Lymphotactin is produced by NK cells and attracts both NK cells and T cells in vivo (1997)

Injection of lymphotactin (Lptn) into the peritoneum caused an influx of lymphocytes at 24 h. Phenotypic analysis of the cellular influx showed that a large proportion of these cells were T lymphocytes; however, a large number of NK cells was also present. This effect of murine Lptn (mLptn) was specific since the cellular influx was blocked with a mLptn-specific mAb. Similar results were observed when Lptn was injected s.c. and the tissue was analyzed by immunohistochemistry using an anti-CD3epsilon mAb. Microchemotaxis assays confirmed that murine NK cells respond to mLptn, and also showed human NK clones to be similarly responsive to recombinant human Lptn (rhLptn). Immunohistochemical analysis of IL-2-activated murine NK cells and Northern analysis of human NK clones revealed that these cells also produce Lptn, suggesting that a self-regulatory migration mechanism exists in NK cells. Together these data confirm, in vivo, the lymphocyte specificity of Lptn previously observed in vitro and extend its chemotactic effects to the NK cell lineage. We also investigated the functional consequences of truncating the carboxyl terminus of hLptn. This truncated molecule (which is missing the carboxyl-terminal 22 amino acids of hLptn) had no detectable activity on human PBLs. In addition, while hLptn was found to attract murine splenocytes in vitro, the carboxyl-terminal truncated hLptn was again inactive on murine splenocytes. This observation indicates the presence of structural features in the carboxyl terminus of Lptn that are necessary for its biologic activity.
Ia-antigen-T-cell interactions for a thymus-independent antigen composed of D amino acids. (1993)

Synthetic polypeptide antigens of L amino acids, although bearing repeating sequences, are thymus-dependent (L-TD), whereas the same polymers composed of D amino acids are thymus-independent (D-TI), probably due to a slower rate of metabolism. Yet we found that lymph-node cells of BALB/c mice immunized with D-TI proliferate in response to it in vitro. To follow T-cell activation by D-TI, we established T-cell hybridomas to D-TI and to its analog composed of L isomers, L-TD, for comparison. The T-cell hybridomas express membrane alpha/beta T-cell receptors and secrete interleukin 2 upon stimulation with the respective antigen. In addition, D-TI-specific hybridomas are stimulated, to a lesser extent, by the L-TD antigen, whereas only some L-TD-specific hybridomas recognize D-TI. Moreover, biotinylated analogs of D-TI and L-TD bind to splenic antigen-presenting cells (APCs) from BALB/c mice. Binding is inhibited by an excess of nonbiotinylated L-TD, and by an excess of a peptide comprising residues 259-271 of the human acetylcholine receptor alpha subunit, which binds to I-Ad and I-Ed molecules without prior processing. Analysis of APC lysates following incubation of the APCs with biotinylated D-TI and L-TD reveals that the biotinylated antigen moiety is associated with Ia molecules. D-TI and L-TD bind to Ia molecules on intact APCs with similar KD values, 5 x 10(-8) M and 3 x 10(-8) M, respectively. However, D-TI has faster kinetics of binding than L-TD, probably due to different processing requirements. Hence, we have demonstrated a major histocompatibility complex class II-mediated T-cell response to a thymus-independent antigen.
The Binding of Calcium to the B-repeat Segment of SdrD, a Cell Surface Protein of Staphylococcus aureus (1998)

In the Sdr family of Staphylococcus aureus cell surface proteins, three recently cloned members (Josefsson, E., McCrea, K., Ni Eidhin, D., O'Connell, D., Cox, J. A., Hook, M., and Foster, T. (1998) Microbiology, in press) display variable numbers of B-repeats, i.e. segments of 110-113 residues that probably make up one folding unit. Each B-repeat contains one conserved EF-hand motif and two acidic stretches. Equilibrium dialysis revealed that segment B1-B5 of SrdD contains 14 Ca2+-binding sites with high affinity ([Ca2+]0.5, 4 microM), whereas flow dialysis yielded 5 sites of high affinity (class I) and 10 of low affinity (class II). The discrepancy could be explained by the slow induction of high affinity in the class II sites. Kinetic experiments using fluorescent Ca2+ indicators corroborated slow binding of Ca2+ at the latter sites. Circular dichroism and Trp fluorescence showed that, whereas the Ca2+ form is well folded, the metal-free form seems strongly disorganized. The Ca2+-induced conformational changes comprise both fast and slow steps, giving thus a structural support for the induction of class II Ca2+-binding sites. The B-repeats may act as rulers or springs that modulate the distance between the interactive A region and the bacterial cell surface.
Blind Source Separation and Independent Component Analysis: A Review (2005)

Blind source separation (BSS) and independent component analysis (ICA) are generally based on a wide class of unsupervised learning algorithms and they found potential applications in many areas from engineering to neuroscience. A recent trend in BSS is to consider problems in the framework of matrix factorization or more general signals decomposition with probabilistic generative and tree structured graphical models and exploit a priori knowledge about true nature and structure of latent (hidden) variables or sources such as spatio-temporal decorrelation, statistical independence, sparseness, smoothness or lowest complexity in the sense e.g., of best predictability. The possible goal of such decomposition can be considered as the estimation of sources not necessary statistically independent and parameters of a mixing system or more generally as finding a new reduced or hierarchical and structured representation for the observed (sensor) data that can be interpreted as physically meaningful coding or blind source estimation. The key issue is to find a such transformation or coding (linear or nonlinear) which has true physical meaning and interpretation. We present a review of BSS and ICA, including various algorithms for static and dynamic models and their applications. The paper mainly consists of three parts: (1) BSS algorithms for static models (instantaneous mixtures); (2) extension of BSS and ICA incorporating with sparseness or non-negativity constraints; (3) BSS algorithms for dynamic models (convolutive mixtures).
Stearyl-norleucine-vasoactive intestinal peptide (VIP): a novel VIP analog for noninvasive impotence treatment. (1994)

The present report relates to pharmaceutical composition for the treatment of male impotence. The transdermal application of a potent derivative of vasoactive intestinal peptide (VIP) coupled to a suitable hydrophobic moiety (e.g. stearyl-VIP) in a suitable ointment composition (e.g. Sefsol) enhances sexual activity and erection formation in a variety of impotence models in rats (sterile rats, diabetic rats, and animals with high blood pressure). Furthermore, exchange of the methionine in position 17 with norleucine enhances biological activity. Thus, stearyl-Nle17-VIP may be considered useful for the treatment of impotence.
Cell-contact-dependent signalling in axon growth and guidance: Eph receptor tyrosine kinases and receptor protein tyrosine phosphatase β (1998)

The growth and guidance of axons involves the recognition of complex environmental cues by receptor proteins on the surface of the growth cone and their interpretation by cellular machinery, leading to changes in cellular behaviour. Recent advances have demonstrated that the ligands for Eph receptor tyrosine kinases, the ephrins, act as repulsive axon guidance cues, and that Eph receptors are required for correct axonal navigation in vivo. Members of the receptor protein tyrosine phosphatase (RPTP) family also play important roles in axon guidance and growth. RPTP beta and Eph receptors interact with cell-surface-bound ligands, and there is increasing evidence that both transmembrane ephrins and contactin, a ligand for RPTP beta, may possess an intrinsic signalling function. Thus, the cell-contact-dependent interactions between these receptors and ligands may lead to initiation of bidirectional signals that regulate axonal growth and migration.
Polymicrobial sepsis selectively activates peritoneal but not alveolar macrophages to release inflammatory mediators (interleukins-1 and -6 and tumor necrosis factor). (1992)

While a number of clinical studies indicate that elevated serum cytokine [interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor (TNF)] levels are associated with enhanced mortality in sepsis, the time course and the role that different macrophage (M phi) populations play in releasing these cytokines remain to be determined. To study this, polymicrobial sepsis was induced in C3H/HeN mice by cecal ligation and puncture (CLP). The animals were then sacrificed at 1, 4, or 24 hr post-CLP. Blood was taken for serum cytokine level determination. Macrophages, of either peritoneal (PM phi) or alveolar (AM phi) origin, were harvested by lavage, and their innate vs. inducible cytokine productive capacities were assessed by incubation with or without endotoxin (lipopolysaccharide; LPS). Serum levels of TNF were significantly enhanced 1 hr post-CLP (CLP = 3.8 +/- 2.4* vs. sham = 0.4 +/- 0.9 U/ml; P less than 0.05 by t test). However, not until 4 hr post-CLP were marked increases in IL-6 observed (CLP = 318.0 +/- 209.0* vs. sham = 1.1 +/- 0.5 U/ml), which remained elevated through 24 hr post-CLP (CLP = 11.3 +/- 15.0* vs. sham = 0.03 +/- 0.02 U/ml). Cytokine release (IL-1, IL-6, TNF) from PM phi (without the addition of LPS) was detectable only in cells harvested 1 h following CLP. Alveolar M phi from septic mice showed little in vivo activation. Septic PM phi IL-1 and IL-6 production was markedly depressed at all time points with LPS stimulation, but TNF release remained unaltered.(ABSTRACT TRUNCATED AT 250 WORDS)
Selective Reductions. VII. Reaction of Lithium Trimethoxyaluminohydride with Selected Organic Compounds Containing Representative Functional Groups1 (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelective Reductions. VII. Reaction of Lithium Trimethoxyaluminohydride with Selected Organic Compounds Containing Representative Functional Groups1Herbert C. Brown and Paul M. WeissmanCite this: J. Am. Chem. Soc. 1965, 87, 24, 5614–5620Publication Date (Print):December 1, 1965Publication History Published online1 May 2002Published inissue 1 December 1965https://pubs.acs.org/doi/10.1021/ja00952a018https://doi.org/10.1021/ja00952a018research-articleACS PublicationsRequest reuse permissionsArticle Views649Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Advanced pancreatic adenocarcinoma: a review of current treatment strategies and developing therapies (2015)

Pancreatic adenocarcinoma is one of the deadliest solid malignancies. A large proportion of patients are diagnosed with locally advanced or metastatic disease at the time of presentation and, unfortunately, this severely limits the number of patients who can undergo surgical resection, which offers the only chance for cure. Recent therapeutic advances for patients with advanced pancreatic cancer have extended overall survival, but prognosis still remains grim. Given that traditional chemotherapy is ineffective in curing advanced pancreatic adenocarcinoma, current research is taking a multidirectional approach in the hopes of developing more effective treatments. This article reviews the major clinical trial data that is the basis for the current chemotherapy regimens used as first- and second-line treatments for advanced pancreatic adenocarcinoma. We also review the current ongoing clinical trials, which include the use of agents targeting the oncogenic network signaling of K-Ras, agents targeting the extracellular matrix, and immune therapies.
Altered Pathogenesis of Porcine Respiratory Coronavirus in Pigs due to Immunosuppressive Effects of Dexamethasone: Implications for Corticosteroid Use in Treatment of Severe Acute Respiratory Syndrome Coronavirus (2007)

The pathogenesis and optimal treatments for severe acute respiratory syndrome (SARS) are unclear, although corticosteroids were used to reduce lung and systemic inflammation. Because the pulmonary pathology of porcine respiratory coronavirus (PRCV) in pigs resembles SARS, we used PRCV as a model to clarify the effects of the corticosteroid dexamethasone (DEX) on coronavirus (CoV)-induced pneumonia. Conventional weaned pigs (n = 130) in one of four groups (PRCV/phosphate-buffered saline [PBS] [n = 41], PRCV/DEX [n = 41], mock/PBS [n = 23], and mock/DEX [n = 25]) were inoculated intranasally and intratracheally with the ISU-1 strain of PRCV (1 x 10(7) PFU) or cell culture medium. DEX was administered (once daily, 2 mg/kg of body weight/day, intramuscularly) from postinoculation day (PID) 1 to 6. In PRCV/DEX pigs, significantly milder pneumonia, fewer PRCV-positive cells, and lower viral RNA titers were present in lungs early at PID 2; however, at PID 4, 10, and 21, severe bronchointerstitial pneumonia, significantly higher numbers of PRCV-positive cells, and higher viral RNA titers were observed compared to results for PRCV/PBS pigs. Significantly lower numbers of CD2(+), CD3(+), CD4(+), and CD8(+) T cells were also observed in lungs of PRCV/DEX pigs than in those of PRCV/PBS pigs at PID 8 and 10, coincident with fewer gamma interferon (IFN-gamma)-secreting cells in the tracheobronchial lymph nodes as determined by enzyme-linked immunospot assay. Our results confirm that DEX treatment alleviates PRCV pneumonia early (PID 2) in the infection but continued use through PID 6 exacerbates later stages of infection (PID 4, 10, and 21), possibly by decreasing cellular immune responses in the lungs (IFN-gamma-secreting T cells), thereby creating an environment for more-extensive viral replication. These data have potential implications for corticosteroid use with SARS-CoV patients and suggest a precaution against prolonged use based on their unproven efficacy in humans, including possible detrimental secondary effects.
Broad spectrum late blight resistance in potato differential set plants MaR8 and MaR9 is conferred by multiple stacked R genes (2011)

Phytophthora infestans is the causal agent of late blight in potato. The Mexican species Solanum demissum is well known as a good resistance source. Among the 11 R gene differentials, which were introgressed from S. demissum, especially R8 and R9 differentials showed broad spectrum resistance both under laboratory and under field conditions. In order to gather more information about the resistance of the R8 and R9 differentials, F1 and BC1 populations were made by crossing Mastenbroek (Ma) R8 and R9 clones to susceptible plants. Parents and offspring plants were examined for their pathogen recognition specificities using agroinfiltration with known Avr genes, detached leaf assays (DLA) with selected isolates, and gene-specific markers. An important observation was the discrepancy between DLA and field trial results for Pi isolate IPO-C in all F1 and BC1 populations, so therefore also field trial results were included in our characterization. It was shown that in MaR8 and MaR9, respectively, at least four (R3a, R3b, R4, and R8) and seven (R1, Rpi-abpt1, R3a, R3b, R4, R8, R9) R genes were present. Analysis of MaR8 and MaR9 offspring plants, that contained different combinations of multiple resistance genes, showed that R gene stacking contributed to the Pi recognition spectrum. Also, using a Pi virulence monitoring system in the field, it was shown that stacking of multiple R genes strongly delayed the onset of late blight symptoms. The contribution of R8 to this delay was remarkable since a plant that contained only the R8 resistance gene still conferred a delay similar to plants with multiple resistance genes, like, e.g., cv Sarpo Mira. Using this "de-stacking" approach, many R gene combinations can be made and tested in order to select broad spectrum R gene stacks that potentially provide enhanced durability for future application in new late blight resistant varieties.
Erratum: Proteomic analysis of microvesicles derived from human colorectal cancer ascites (2011)

The correct affiliation for Dr. Do-Yong Choi, the first author of this paper, is: 2Department of Molecular Biotechnology, WCU program, Konkuk University, Seoul, Republic of Korea The list of keywords should read as: Ascites / Colorectal cancer / Exosomes / Microvesicles On page 2746, second column, first paragraph, the sentence following the reference to Fig. 1D should read as: Microvesicle-associated marker proteins including Alix, Tsg101, CD81, β-actin, ezrin, intercellular adhesion molecule-1, and catenin β-1 were detected in the purified microvesicles [2, 13, 14]. Likewise on page 2747, the last sentence in the first column should read as: We found that CRC ascites-derived microvesicles contain several adherens junction and tight-junction proteins including catenin α-1, catenin β-1, junctional plakoglobin, and vinculin. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Über isoindigoide Farbstoffe der Pyrrol‐Reihe (1967)

Abstract Der durch Lactam‐Ringschluß aus Phenylketobuttersäure erhaltene Farbstoff besitzt die von Entdecker Kugel vorgeschlagene Struktur 4 eines Phenylpyrrolisoindigos P‐II und ist identisch mit der aus dem Sauerstoff‐Analogon, dem Pechmann‐Farbstoff 6 , erhältlichen Verbindung. Außer P‐II entsteht ein Kondensationsprodukt 5 des hypothetischen Phenyl‐pyrrolons mit Phenylketobuttersäure, das über eine extrem leicht verlaufende Retrocrotonisierungs‐Retroaldol‐Reaktion auch in P‐II übergeführt werden kann. Für Pyrrol‐indol‐isoindigo‐Derivate werden neue Synthesewege aufgezeigt. Pyrrolylpyrrolone 19, 20 und daraus Pyrrolylpyrrol‐isoindigo‐Derivate 27, 28 werden nach einer neuen Methode erstmals erhalten; es sind Abkömmlinge des α.β.α‐verknüpften Tetrapyrrols.
Online Adaptation of Convolutional Neural Networks for Video Object Segmentation (2017)

We tackle the task of semi-supervised video object segmentation, i.e. segmenting the pixels belonging to an object in the video using the ground truth pixel mask for the first frame. We build on the recently introduced one-shot video object segmentation (OSVOS) approach which uses a pretrained network and fine-tunes it on the first frame. While achieving impressive performance, at test time OSVOS uses the fine-tuned network in unchanged form and is not able to adapt to large changes in object appearance. To overcome this limitation, we propose Online Adaptive Video Object Segmentation (OnAVOS) which updates the network online using training examples selected based on the confidence of the network and the spatial configuration. Additionally, we add a pretraining step based on objectness, which is learned on PASCAL. Our experiments show that both extensions are highly effective and improve the state of the art on DAVIS to an intersection-over-union score of 85.7%.
Preparation and Photochemistry of 5,6-Cyclopropyluridines and of Bicyclic Isomers of Thymines (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation and Photochemistry of 5,6-Cyclopropyluridines and of Bicyclic Isomers of ThyminesT. Kunieda and B. WitkopCite this: J. Am. Chem. Soc. 1969, 91, 27, 7751–7752Publication Date (Print):December 1, 1969Publication History Published online1 May 2002Published inissue 1 December 1969https://pubs.acs.org/doi/10.1021/ja50001a043https://doi.org/10.1021/ja50001a043research-articleACS PublicationsRequest reuse permissionsArticle Views96Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
<title>Wavefront coding: jointly optimized optical and digital imaging systems</title> (2000)

Many of the limitations of traditional optical-only imaging systems can be eliminated with jointly optimized optical and digital imaging systems. Jointly optimized optical and digital imaging systems exploit the complementary aspects of optics and digital signal processing to form systems with characteristics not possible with traditional optics-only systems. For example, in traditional imaging systems light gathering and large depth of field are competing goals and are inversely related. On the other hand, in optimized optical/digital imaging systems light gathering and large depth of field can be independent parameters. Instead of requiring a small aperture to produce a large depth of field, a large aperture and a large depth of field are both possible and practical. We can jointly optimized optical and digital imaging systems Wavefront Coded imaging systems. Concepts of Wavefront Coding are illustrated below through an athermalized, refractive, silicon/germanium IR imaging system with aluminum optical mounts subject to an ambient temperature range of -20 degree(s)C to +70 degree(s)C.