gebhart/patrepeval huggingface dataset:
Scanning spreading resistance microscopy and spectroscopy for routine and quantitative two-dimensional carrier profiling (2002)

As emphasized in the International Technological Roadmap for Semiconductors (ITRS), two-dimensional carrier profiling is one of the key elements in support of technology development. Scanning spreading resistance microscopy (SSRM) has been demonstrated to have attractive concentration sensitivity, an easy quantification, and is applicable to complementary metal–oxide–semiconductor Si and InP structures. Its commercial implementation and availability together with an ample supply of appropriate (diamond based) tips has enabled its more widespread use during recent years. In this article we propose a number of measurement procedures and software tools for its more reliable and fast routine application. First we present a program for the automatic generation of calibration curves and the fast quantification of one-dimensional and two-dimensional resistivity (and carrier) profiles. In view of the large tip consumption, a fast evaluation and calibration of newly mounted conductive tips is a major issue. Furthermore, the fast extraction of the underlying carrier distributions leads to an easier data interpretation. We also propose an overview of the implementation and of the applications of the scanning spreading resistance spectroscopy (SSRS), where a full I–V curve is collected at each measurement point. SSRS proves to be particularly interesting to study the point-contact characteristics (in scanning mode) and simplifies significantly the junction delineation. SSRS can also be used for the selection of the optimal bias settings for the quantification procedure.
Immunogenically optimized peptides derived from natural mutants of HIV CTL epitopes and peptide combinatorial libraries (2008)

Two strategies were aimed at identifying immunogenically optimized peptides for the potential use in the formulation of an effective prophylactic or therapeutic HIV-1 vaccine. Three CTL epitopes were investigated: Gag p24(19-27) TV9, Gag p17(77-85) SL9, and RT(309-317) IV9. The first strategy derives from the hypothesis that a number of rare mutant CTL epitopes of HIV-1 may be more immunogenic than the common ones. As such, these rare mutant sequences might be highly effective in generating cross reactive anti-HIV-1 CTL responses against a range of mutant sequences. As anticipated, several rare mutant peptide sequences were identified that generated strong CTL responses against both the consensus sequences and several naturally occurring mutants in human PBL cultures primed ex vivo and in HLA-A2 transgenic mice immunized in vivo. Finally, to reach beyond the sequence diversity of the "natural" library of mutated sequences, a synthetic combinatorial peptide library was screened with a TV9 specific T-cell line; this resulted in the identification of an immunogenically optimized mimic peptide sequence that provoked highly effective CTL immune responses against TV9 and mutants. Sequence homologies between the natural mutants and synthetic mimic may provide insight into key contact positions in the MHC/TCR/peptide complex.
Noncovalent Attachment of NAD+ Cofactor onto Carbon Nanotubes for Preparation of Integrated Dehydrogenase-Based Electrochemical Biosensors (2010)

This study describes a facile approach to the preparation of integrated dehydrogenase-based electrochemical biosensors through noncovalent attachment of an oxidized form of beta-nicotinamide adenine dinucleotide (NAD(+)) onto carbon nanotubes with the interaction between the adenine subunit in NAD(+) molecules and multiwalled carbon nanotubes (MWCNTs). X-ray photoelectron spectroscopic and cyclic voltammetric results suggest that NAD(+) is noncovalently attached onto MWCNTs to form an NAD(+)/MWCNT composite that acts as the electronic transducer for the integrated dehydrogenase-based electrochemical biosensors. With glucose dehydrogenase (GDH) as a model dehydrogenase-based recognition unit, electrochemical studies reveal that glucose is readily oxidized at the GDH/NAD(+)/MWCNT-modified electrode without addition of NAD(+) in the phosphate buffer. The potential for the oxidation of glucose at the GDH/NAD(+)/MWCNT-modified electrode remains very close to that for NADH oxidation at the MWCNT-modified electrode, but it is more negative than those for the oxidation of glucose at the MWCNT-modified electrode and for NADH oxidation at a bare glassy carbon electrode. These results demonstrate that NAD(+) molecules stably attached onto MWCNTs efficiently act as the cofactor for the dehydrogenases. MWCNTs employed here not only serve as the electronic transducer and the support to confine NAD(+) cofactor onto the electrode surface, but also act as the electrocatalyst for NADH oxidation in the dehydrogenase-based electrochemical biosensors. At the GDH/NAD(+)/MWCNT-based glucose biosensor, the current is linear with the concentration of glucose being within a concentration range from 10 to 300 microM with a limit of detection down to 4.81 microM (S/N = 3). This study offers a facile and versatile approach to the development of integrated dehydrogenase-based electrochemical devices, such as electrochemical biosensors and biofuel cells.
gridlock , an HLH Gene Required for Assembly of the Aorta in Zebrafish (2000)

The first artery and vein of the vertebrate embryo assemble in the trunk by migration and coalescence of angioblasts to form endothelial tubes. The gridlock (grl) mutation in zebrafish selectively perturbs assembly of the artery (the aorta). Here it is shown that grl encodes a basic helix-loop-helix (bHLH) protein belonging to the Hairy/Enhancer of the split family of bHLH proteins. The grl gene is expressed in lateral plate mesoderm before vessel formation, and thereafter in the aorta and not in the vein. These results suggest that the arterial endothelial identity is established even before the onset of blood flow and implicate the grl gene in assignment of vessel-specific cell fate.
HOW DO FISH OILS AFFECT VASCULAR FUNCTION? (1995)

1. This is a review on the mechanisms by which fish oils affect vascular function and how such changes contribute to their documented cardioprotective effects. 2. Evidence that fish oils depress vascular responses to contractile agents will be examined. It is concluded that this effect of fish oils is mediated predominantly by alterations in prostanoid profile. 3. Effects of fish oils on arterial relaxation are discussed with particular emphasis on endothelium dependent relaxation. It is suggested that the functional impairment of endothelium dependent relaxation documented in a number of cardiovascular disease states can be reversed by dietary fish oils. 4. In addition, possible effects of fish oils on growth factors, inositol trisphosphate and lipid metabolism, the sympathetic nervous system, rheological and membrane properties and inducible nitric oxide are reviewed.
A highly conserved cryptic epitope in the receptor-binding domains of SARS-CoV-2 and SARS-CoV (2020)

ABSTRACT The outbreak of COVID-19, which is caused by SARS-CoV-2 virus, continues to spread globally, but there is currently very little understanding of the epitopes on the virus. In this study, we have determined the crystal structure of the receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) protein in complex with CR3022, a neutralizing antibody previously isolated from a convalescent SARS patient. CR3022 targets a highly conserved epitope that enables cross-reactive binding between SARS-CoV-2 and SARS-CoV. Structural modeling further demonstrates that the binding site can only be accessed when at least two RBDs on the trimeric S protein are in the “up” conformation. Overall, this study provides structural and molecular insight into the antigenicity of SARS-CoV-2. ONE SENTENCE SUMMARY Structural study of a cross-reactive SARS antibody reveals a conserved epitope on the SARS-CoV-2 receptor-binding domain.
Isolation of a Novel Receptor cDNA Establishes the Existence of Two PDGF Receptor Genes (1989)

A genomic sequence and cloned complementary DNA has been identified for a novel receptor-like gene of the PDGF receptor/CSF1 receptor subfamily (platelet-derived growth factor receptor/colony-stimulating factor type 1 receptor). The gene recognized a 6.4-kilobase transcript that was coexpressed in normal human tissues with the 5.3-kilobase PDGF receptor messenger RNA. Introduction of complementary DNA of the novel gene into COS-1 cells led to expression of proteins that were specifically detected with antiserum directed against a predicted peptide. When the new gene was transfected into COS-1 cells, a characteristic pattern of binding of the PDGF isoforms was observed, which was different from the pattern observed with the known PDGF receptor. Tyrosine phosphorylation of the receptor in response to the PDGF isoforms was also different from the known receptor. The new PDGF receptor gene was localized to chromosome 4q11-4q12. The existence of genes encoding two PDGF receptors that interact in a distinct manner with three different PDGF isoforms likely confers considerable regulatory flexibility in the functional responses to PDGF.
Vena Tech-LGM filter: long-term results of a prospective study. (1993)

Efficiency and tolerance of the Vena Tech-LGM filter were assessed in 142 patients prospectively studied during a 2-6-year follow-up period. No patients were lost to follow-up, and 137 (91.3%) scheduled control examinations were performed. Fifty-three patients died of causes unrelated to thromboembolic disease. Pulmonary embolism was suspected in five surviving patients (3.5%). Sixteen retractions (18.4%), 16 intracaval migrations (18.4%), and one tilt were observed. Filter patency studied at Doppler ultrasonography, with or without phlebocavography, gave the following results according to the Kaplan-Meier actuarial method: 92% patency after 2 years of follow-up, 80% after 4 years, and 70% after 6 years. Vena Tech-LGM filter obstruction was related to retraction (P < 10(-6)) or distal migration (P < .004). Occurrence of trophic disease in the lower limbs during the follow-up period was related to the initial level of deep venous thrombosis (P = .03) and had no relation to the patency of the filter. The filter was effective in preventing pulmonary embolism, with 70% demonstrating long-term patency, and no deleterious effects were imputed to obstructions observed during follow-up.
Detection of Humoral Antibody Response to Polyoma Tumor-Specific Cell-Surface Antigen (1970)

Journal Article Detection of Humoral Antibody Response to Polyoma Tumor-Specific Cell-Surface Antigen Get access Chou-Chik Ting, Chou-Chik Ting Immunology Branch, National Cancer Institute,4Bethesda, Maryland 20014 Search for other works by this author on: Oxford Academic PubMed Google Scholar Ronald B. Herberman Ronald B. Herberman Immunology Branch, National Cancer Institute,4Bethesda, Maryland 20014 Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 44, Issue 3, March 1970, Pages 729–737, https://doi.org/10.1093/jnci/44.3.729 Published: 01 March 1970 Article history Received: 17 November 1969 Accepted: 02 December 1969 Published: 01 March 1970
Protection by Group II Phospholipase A2 Against Staphylococcus aureus (1999)

Group II phospholipase A2 (PLA2) is an enzyme that has marked antibacterial properties in vitro. To define the role of group II PLA2 in the defense against Staphylococcus aureus, we studied host responses in transgenic mice expressing human group II PLA2 and group II PLA2-deficient C57BL/6J mice in experimental S. aureus infection. After the administration of S. aureus, the transgenic mice showed increased expression of group II PLA2 mRNA in the liver and increased concentration of group II PLA2 in serum, whereas the PLA2-deficient mice completely lacked the PLA2 response. Expression of human group II PLA2 resulted in reduced mortality and improved the resistance of the mice by killing the bacteria as indicated by low numbers of live bacteria in their tissues. Human group II PLA2 was responsible for the bactericidal activity of transgenic mouse serum. These results suggest a possible role for group II PLA2 in the innate immunity against S. aureus infection.
Participation of the Humoral Immune System in the Myeloma-Specific Transplantation Resistance (1978)

Abstract Studies are reported that examine the participation of humoral and cellular responses in the myeloma-specific transplantation resistance induced by preimmunization of BALB/c mice with purified myeloma protein M315. A plasmacytoma spleen colony-forming assay was used to provide a quantitative estimate of the tumor immunity in recipients of unfractionated spleen cells or serum from mice immunized four to seven times with M315. The primary findings were: 1) that the tumor immunity can be transferred by immune spleen cells provided that a boost of M315 is given to adoptive hosts, and 2) that passively transferred serum containing anti-M315 idiotype antibody (a-Id315) can also inhibit tumor growth. Adoptive transfer was successful in the presence of minute amounts of a-Id315, whereas passive transfer required relatively large amounts of activity. The passive transfer experiments involved an extended injection schedule and thus could not discriminate between direct effects of antibody or idiotype-specific humoral substances on tumor cells or inductive effects on the cellular immune system. Experiments examining the properties of the immune elements involved in the transplantation immunity demonstrated that, once established, they are resistant to acute radiation (to 800 R) and cortisone damage.
The Spread of English and Its Impact (2009)

Body Mass Index (BMI) is a risk indicator for some eye diseases. However, the association between BMI and Visual Impairment (VI) was not quite certain in Chinese students. Our aim was to assess the relationship between BMI and VI with a cross-sectional study. A total of 3771 students aged 6-21 years, including 729 with VI, were sampled from 24 schools in Huangpi District of central China to participate in the study. A multistage stratified cluster random sampling was adopted. Each of the students answered a questionnaire and had physical and eye examinations. The association between BMI and VI was examined with logistic regression and threshold effect analysis. The prevalence of VI was 19.33% (729/3771). Compared to normal and underweight, overweight/obese students showed a stronger relation with VI in age- and sex-adjusted (Odds Ratio (OR) = 16.16, 95% Confidence Interval (CI): 12.37-21.09, <i>p</i> < 0.001) and multivariable models (OR = 8.32, 95% CI: 6.13-11.30, <i>p</i> < 0.001). There was a nonlinear dose-response relation between levels of BMI and the prevalence of VI (<i>p</i> < 0.001). A high level of BMI (≥19.81 kg/m²) was associated with a higher VI prevalence (adjusted OR = 1.20, 95% CI: 1.15-1.25, <i>p</i> < 0.001). In conclusion, the study demonstrated BMI levels were significantly associated with the prevalence of VI.
Bolometric and nonbolometric radio frequency detection in a metallic single-walled carbon nanotube (2011)

We characterize radio frequency detection in a high-quality metallic single-walled carbon nanotube. At a bath temperature of 77 K, only bolometric (thermal) detection is seen. At a bath temperature of 4.2 K and low bias current, the response is due instead to the electrical nonlinearity of the non-Ohmic contacts. At higher bias currents, the contacts recover Ohmic behavior and the observed response agrees well with the calculated bolometric responsivity. The bolometric response is expected to operate at terahertz frequencies, and we discuss some of the practical issues associated with developing high frequency detectors based on carbon nanotubes.
Botulinum Toxin Type A Injections Into the Trigone to Treat Idiopathic Overactive Bladder do Not Induce Vesicoureteral Reflux (2007)

Botulinum toxin A injection into the trigone does not induce de novo vesicoureteral reflux in patients with nonneurogenic overactive bladder. The therapeutic value of this approach remains to be confirmed and compared to other injection designs.
Role of 2',5'-oligo(adenylic acid) polymerase in the degradation of ribonucleic acid linked to double-stranded ribonucleic acid by extracts of interferon-treated cells (1980)

RNA covalently linked to double-stranded RNA (dsRNA) is preferentially degraded in extracts of interferon-treated HeLa cells [Nilsen, T. W., & Baglioni, C. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 2600-2604]. The size of the dsRNA required for this preferential degradation has been determined by annealing poly(I) of known length to the poly(C) tract of encephalomyocarditis virus (EMCV) RNA or by annealing poly(U) to poly(A) of known length of vesicular stomatitis virus mRNA. The dsRNA must be longer than about 60 base pairs to observe the preferential degradation of RNA. Moreover, triple-stranded regions that do not activate synthesis of 2',5'-oligo(A) and ethidium bromide, which intercalates in dsRNA and blocks 2',5'-olido(A) polymerase activation, prevent this degradation. Ethidium also blocks the degradation of the replicative intermediate of EMCV by extracts of interferon-treated cells. These experiments indicate that synthesis of 2',5'-oligo(A) is required for the degradation of RNA linked to dsRNA. The 2',5'-oligo(A)-dependent endonuclease does not cleave single- or double-stranded DNA, nor does it cleave homopolyribonucleotides. The potential role of the 2',5'-oligo(A) polymerase/endonuclease system in the inhibition of viral RNA replication is discussed.
Derivatization of Carbon Surfaces by Anodic Oxidation of Arylacetates. Electrochemical Manipulation of the Grafted Films (1997)

Electrochemical oxidation of the corresponding carboxylates (Kolbe reaction) offers a simple and efficient means to solidly attach a monolayer of arylmethyl groups on carbon surfaces. The starting molecules are easily accessible, and the presence of appropriate substituents on the phenyl rings makes this type of derivatization a convenient starting point for further chemical modifications. The extent of the surface modification can be followed by cyclic voltammetry as a function of the number of successive potential cycles. The presence of the groups on the surface, their arrangement, and their concentration are determined by the same technique as well as by X-ray photoelectron spectroscopy and scanning electron tunneling microscopy after transfer of the derivatized electrode into a pure electrolyte solution. The fact that reductive and oxidative cyclic voltammograms of the derivatized electrodes can be obtained demonstrates that the grafted groups are in electrical contact with the electrode by means of electron hopping to or from the aryl moieties they contain. After the electrode has been passivated at the potential whereby anodic oxidation occurs, a second, more positive wave appears. Oxidation at the potential of this second wave triggers a further grafting process leading to a denser film. Electrochemical manipulations allow not only the attachment of electroactive films on the carbon surface but also their erasing by setting the electrode potential at a value where the attached groups are oxidized.
Involvement of gamma interferon in antibody enhancement by adjuvants (1990)

In a previous study the adjuvant action of a monophosphoryl lipid A, a nontoxic derivative of endotoxic lipopolysaccharide (LPS), was found to be negated by a monoclonal anti-gamma interferon (anti-IFN-gamma) antibody. The present investigation centered on three other adjuvants of diverse microbial origins, testing for their capacity to affect the release of IFN-gamma as an explanation for their antibody-enhancing action. The adjuvant action of each of the three, a wild-type LPS, synthetic poly(A)-poly(U) complexes, and a synthetic muramyl dipeptide, n-acetylmuramyl-L-alanyl-D-glutaminyl-n-butyl ester (murabutide), was transferable by adjuvant-stimulated T cells to normal spleen cells on coculture. Supernatant fluids from these T cells contained increased levels of IFN-gamma. Addition of a monoclonal anti-IFN-gamma antibody to adjuvant-stimulated spleen cell cultures reduced the adjuvant action by approximately one-half. Removal of natural killer cells from spleen cell populations prior to culture with antigen had no effect on the enhancement induced by LPS and monophosphoryl lipid A. It was concluded that the enhancement induced by the adjuvants LPS, poly(A)-poly(U), and murabutide is mediated in part by their action on T cells resulting in release of IFN-gamma suggesting activation of a common transmembrane signal.
A new frequency channel assignment algorithm in high capacity mobile communication systems (1982)

A new algorithm for frequency channel assignment in mobile radio communication is proposed. The algorithm uses flexible fixed channel assignment which enables the calls having all their nominal channels busy to borrow channels from the neighboring cells provided that co-channel interference will not result. The borrowed channel cannot be used in three interfering cells; therefore reassignment strategy is used when a call is terminated on a nominal channel to switch a call in progress in a borrowed channel to that nominal one and set free the borrowed channel in the three interfering cells. Thus the traffic carried on borrowed channels is minimized. Also different call switching strategies are proposed to give priority to some channels to be used by the nominal cells and other channels to be borrowed by neighboring cells. These strategies considerably reduce the blocking probability. A simulation study of the algorithm was carried out on 49 hexagonal zones having uniform traffic density. Also a combined telephone and dispatch traffic was included in the simulation study. The results showed that the new algorithm is better than the other channel assignment schemes. This can be applied in high capacity mobile communications systems to utilize the spectrum efficiently.
Curves and surfaces for CAGD a practical guide (2002)

This fifth edition has been fully updated to cover the many advances made in CAGD and curve and surface theory since 1997, when the fourth edition appeared. Material has been restructured into theory and applications chapters. The theory material has been streamlined using the blossoming approach; the applications material includes least squares techniques in addition to the traditional interpolation methods. In all other respects, it is, thankfully, the same. This means you get the informal, friendly style and unique approach that has made Curves and Surfaces for CAGD: A Practical Guide a true classic. The book's unified treatment of all significant methods of curve and surface design is heavily focused on the movement from theory to application. The author provides complete C implementations of many of the theories he discusses, ranging from the traditional to the leading-edge. You'll gain a deep, practical understanding of their advantages, disadvantages, and interrelationships, and in the process you'll see why this book has emerged as a proven resource for thousands of other professionals and academics. * Provides authoritative and accessible information for those working with or developing computer-aided geometric design applications. * Covers all significant CAGD curve and surface design techniques-from the traditional to the experimental. * Includes a new chapter on recursive subdivision and triangular meshes. * Presents topical programming exercises useful to professionals and students alike. * Offers complete C implementations of many of the book's examples via a companion Web site
Pyrrolidinoindoline Alkaloids from Psychotria colorata (1998)

Fractionation of an alkaloid extract of Psychotria colorata flowers led to the isolation of six alkaloids, identified by UV, 1D and 2D NMR, and MS as (-)-calycanthine, isocalycanthine, (+)-chimonanthine, hodgkinsine, quadrigemine C, and a new alkaloid (1), whose structure was deduced by X-ray analysis to be (8-8a),(8'-8'a)-tetradehydroisocalycanthine 3a(R), 3'a(R).