gebhart/patrepeval huggingface dataset:
SALL4 is a robust stimulator for the expansion of hematopoietic stem cells (2011)

HSCs are rare cells that have the unique ability to self-renew and differentiate into cells of all hematopoietic lineages. The lack of donors and current inability to rapidly and efficiently expand HSCs are roadblocks in the development of successful cell therapies. Thus, the challenge of ex vivo human HSC expansion remains a fertile and critically important area of investigation. Here, we show that either SALL4A- or SALL4B-transduced human HSCs obtained from the mobilized peripheral blood are capable of rapid and efficient expansion ex vivo by >10 000-fold for both CD34(+)/CD38(-) and CD34(+)/CD38(+) cells in the presence of appropriate cytokines. We found that these cells retained hematopoietic precursor cell immunophenotypes and morphology as well as normal in vitro or vivo potential for differentiation. The SALL4-mediated expansion was associated with enhanced stem cell engraftment and long-term repopulation capacity in vivo. Also, we demonstrated that constitutive expression of SALL4 inhibited granulocytic differentiation and permitted expansion of undifferentiated cells in 32D myeloid progenitors. Furthermore, a TAT-SALL4B fusion rapidly expanded CD34(+) cells, and it is thus feasible to translate this study into the clinical setting. Our findings provide a new avenue for investigating mechanisms of stem cell self-renewal and achieving clinically significant expansion of human HSCs.
Visualizing Metabolically Labeled Glycoconjugates of Living Cells by Copper‐Free and Fast Huisgen Cycloadditions (2008)

CuI-free click: 4-Dibenzocyclooctynol reacts, in the absence of a CuI catalyst, exceptionally fast with azido-containing saccharides and amino acids to give stable triazoles. A biotin-modified derivative is ideally suited for visualizing and tracking glycoconjugates of living cells that are metabolically labeled with azido-containing monosaccharides (see image). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z705456_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Small and Practical BERT Models for Sequence Labeling (2019)

Henry Tsai, Jason Riesa, Melvin Johnson, Naveen Arivazhagan, Xin Li, Amelia Archer. Proceedings of the 2019 Conference on Empirical Methods in Natural Language Processing and the 9th International Joint Conference on Natural Language Processing (EMNLP-IJCNLP). 2019.
Factor Xa inhibitors (1999)

Factor Xa is a key enzyme in the production of thrombin from its precursor prothrombin and is an important target for antithrombotic drug development. The patent literature for the last four to five years illustrates a variety of peptide and non-peptide Factor Xa inhibitors, including small proteins isolated from natural sources, rationally designed peptides and peptidomimetics based on substrate cleavage sites, and non-peptide small molecules derived from screening and rational design. This report reviews the status of the patent literature in the field of Factor Xa inhibitors through 1998.
Geometric methods in computational fluid dynamics (1980)

General methods for the construction of geometric computational fluid dynamic algorithms are presented which simulate a variety of flow fields in various nontrivial regions. Included are: basic developments with tensors; various forms for the equations of motion; generalized numerical methods and boundary conditions; and methods for mesh generation to meet the strong geometric constraints of turbomachines. Coordinate generation is shown generally to yield mesh descriptions from one or more transformations that are smoothly joined together to form a composite mesh.
Monolithically integrated K/Ka array-based direction finding subsystem (2012)

A wideband K through Ka-band, surface micromachined, monolithically integrated direction finding (DF) front-end is demonstrated and used to determine the angle of arrival of an incoming plane wave utilizing phase/amplitude comparison. The designed subsystem employs a two-element Vivaldi antenna array directly connected with a micro-coaxial 180° hybrid to produce the desired beams. Measurements confirm that the developed front-end operate from 18 to 40 GHz.
Functional Copy-Number Alterations in Cancer (2008)

Understanding the molecular basis of cancer requires characterization of its genetic defects. DNA microarray technologies can provide detailed raw data about chromosomal aberrations in tumor samples. Computational analysis is needed (1) to deduce from raw array data actual amplification or deletion events for chromosomal fragments and (2) to distinguish causal chromosomal alterations from functionally neutral ones. We present a comprehensive computational approach, RAE, designed to robustly map chromosomal alterations in tumor samples and assess their functional importance in cancer. To demonstrate the methodology, we experimentally profile copy number changes in a clinically aggressive subtype of soft-tissue sarcoma, pleomorphic liposarcoma, and computationally derive a portrait of candidate oncogenic alterations and their target genes. Many affected genes are known to be involved in sarcomagenesis; others are novel, including mediators of adipocyte differentiation, and may include valuable therapeutic targets. Taken together, we present a statistically robust methodology applicable to high-resolution genomic data to assess the extent and function of copy-number alterations in cancer.
IGF-I- and IGFBP-3-Expression in Cultured Human Preadipocytes and Adipocytes (2000)

The expression and secretion of IGF-I and IGFBP-3 were investigated in cultured human preadipocytes and in in vitro differentiated adipocytes derived from human subcutaneous adipose tissue under chemically defined culture conditions. Human preadipocytes expressed mRNAs for IGF-I and IGFBP-3 and secreted the corresponding proteins into the culture medium as measured by sensitive radioimmunoassays. In human adipocytes; specific mRNA-expression was comparable to that found in preadipocytes, but IGF-I secretion was increased 10-fold (3.87 +/- 0.69 vs. 0.41 +/- 0.11 ng/ml/10(6) cells/48 hrs, p < 0.05) and IGFBP-3 secretion 2.5-fold (7.34+/-1.15 vs. 3.27+/-0.38 ng/ml/10(6) cells/48 hrs, p<0.05) in the presence of adipogenic medium probably resulting in an increase of unbound IGF-I. Under serum-free, chemically defined conditions human growth hormone (hGH) and insulin were found to be positive regulators and cortisol was found to be a negative regulator of IGF-I and IGFBP-3 secretion in preadipocytes. In cultured human adipocytes, hGH showed no effect on IGF-I and IGFBP-3 secretion, whereas insulin stimulated and cortisol inhibited the secretion of both proteins. We conclude that IGF-I and IGFBP-3 may not only exert their actions in human adipose tissue via circulation, but also in an auto/paracrine way.
CD69 is expressed on platelets and mediates platelet activation and aggregation. (1990)

CD69, a surface dimer so far considered an early activation antigen restricted to lymphocytes, was found constitutively expressed on human platelets. Biochemical analysis revealed that platelet CD69 appears on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a broad 55-65-kD band, in which three 55-, 60-, and 65-kD components were detectable when nonreduced, and as two 28- and 32-kD bands when reduced, corresponding to the two disulfide-linked chains of the dimer. It therefore closely resembles lymphoid CD69, although the resolution of the three bands under nonreducing conditions is not usually seen in lymphoid cells. Moreover, as CD69 expressed on activated lymphocytes and CD3bright thymocytes, both chains are constitutively phosphorylated. CD69 stimulation by anti-Leu-23 monoclonal antibodies induced platelet aggregation in a dose-dependent fashion. This effect was associated with Ca2+ influx and platelet degranulation, as revealed by adenosine triphosphate release. In addition, CD69 stimulation in platelets induced production of thromboxane B2 and PGE2, suggesting activation of arachidonic acid metabolism by cycloxygenase. As observed for CD69-mediated T cell activation, platelet activation through CD69 requires molecular crosslinking. These results suggest that CD69 may function as an activating molecule on platelets, as on lymphocytes, and point toward a more general role of this surface dimer in signal transduction.
Cyclodialysis with Hydroxyethyl Methacrylate Capillary Strip (HCS) (1972)

Research Articles| March 26 2010 Cyclodialysis with Hydroxyethyl Methacrylate Capillary Strip (HCS): Animal Experiments with a New Approach in Glaucoma Drainage Surgery Subject Area: Ophthalmology L. Krejčí L. Krejčí Second Eye Department, Charles University, Prague (Dir.: Doc. Dr. Sc. J. Votočková) Search for other works by this author on: This Site PubMed Google Scholar Ophthalmologica (1972) 164 (2): 113–121. https://doi.org/10.1159/000306718 Article history Published Online: March 26 2010 Content Tools Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation L. Krejčí; Cyclodialysis with Hydroxyethyl Methacrylate Capillary Strip (HCS): Animal Experiments with a New Approach in Glaucoma Drainage Surgery. Ophthalmologica 1 February 1972; 164 (2): 113–121. https://doi.org/10.1159/000306718 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsOphthalmologica Search Advanced Search This content is only available via PDF. 1972Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Article PDF first page preview Close Modal You do not currently have access to this content.
A model of configuration complexity and its application to a change management system (2005)

The complexity of configuring computing systems is a major impediment to the adoption of new information technology (IT) products and greatly increases the cost of IT services. This paper develops a model of configuration complexity and demonstrates its value for a change management system. The model represents systems as a set of nested containers with configuration controls. From this representation, we derive various metrics that indicate configuration complexity, including execution complexity, parameter complexity, and memory complexity. We apply this model to a J2EE-based enterprise application and its associated middleware stack to assess the complexity of the manual configuration process for this application. We then show how an automated change management system can greatly reduce configuration complexity.
The Netflix Prize (2007)

Netflix released a dataset containing 100 million anonymous movie ratings and challenged the data mining, machine learning and computer science communities to develop systems that could beat the accuracy of its recommendation system, Cinematch. We briefly describe the challenge itself, review related work and efforts, and summarize visible progress to date. Other potential uses of the data are outlined, including its application to the KDD Cup 2007.
Electrical damage induced by ion beam etching of GaAs (1988)

We have examined the electrical damage induced in GaAs by ion milling and ion beam assisted etching, relevant to the fabrication of small conducting structures. The depth of the damage was measured by Schottky barrier measurements with in situ deposited gold contacts and by resistance and mobility measurements of etched two-dimensional electron gas structures. The effect of exposed etched sidewalls on the conductivity of narrow wires was examined for GaAs/AlGaAs structures. We find that it is possible to create wires narrower than surface depletion lengths by defining the structures through ion beam induced damage without exposing the sidewalls. In particular, narrow conducting wires can be defined solely by etching the thin-undoped-GaAs cap layer atop the modulation doped material.
Cutting Edge: Peripheral Neuropeptides Attract Immature and Arrest Mature Blood-Derived Dendritic Cells (2001)

Dendritic cells (DC) are highly motile and play a key role in mediating immune responses in various tissues and lymphatic organs. We investigated locomotion of mononuclear cell-derived DC at different maturation stages toward gradients of sensory neuropeptides in vitro. Calcitonin gene-related peptide, vasoactive intestinal polypeptide, secretin, and secretoneurin induced immature DC chemotaxis comparable to the potency of RANTES, whereas substance P and macrophage-inflammatory protein-3beta stimulated immature cell migration only slightly. Checkerboard analyses revealed a true chemotactic response induced by neuropeptides. Upon maturation of DC, neuropeptides inhibited spontaneous, macrophage-inflammatory protein-3beta- and 6Ckine-induced cell migration. Maturation-dependent changes in migratory behavior coincided with distinct neuropeptide-induced signal transduction in DC. Peripheral neuropeptides might guide immature DC to peripheral nerve fibers where high concentrations of these peptides can arrest the meanwhile matured cells. It seems that one function of sensory nerves is to fasten DC at sites of inflammation.
Expression and Characterization of Group AStreptococcusExtracellular Cysteine Protease Recombinant Mutant Proteins and Documentation of Seroconversion during Human Invasive Disease Episodes (1998)

A recent study with isogenic strains constructed by recombinant DNA strategies unambiguously documented that a highly conserved extracellular cysteine protease expressed by Streptococcus pyogenes (group A Streptococcus [GAS]) is a critical virulence factor in a mouse model of invasive disease (S. Lukomski, S. Sreevatsan, C. Amberg, W. Reichardt, M. Woischnik, A. Podbielski, and J. M. Musser, J. Clin. Invest. 99:2574-2580, 1997). To facilitate further investigations of the streptococcal cysteine protease, recombinant proteins composed of a 40-kDa zymogen containing a C192S amino acid substitution that ablates enzymatic activity, a 28-kDa mature protein with the C192S replacement, and a 12-kDa propeptide were purified from Escherichia coli containing His tag expression vectors. The recombinant C192S zymogen retained apparently normal structural integrity, as assessed by the ability of purified wild-type streptococcal cysteine protease to process the 40-kDa molecule to the 28-kDa mature form. All three recombinant purified proteins retained immunologic reactivity with polyclonal and monoclonal antibodies. Humans with a diverse range of invasive disease episodes (erysipelas, cellulitis, pneumonia, bacteremia, septic arthritis, streptococcal toxic shock syndrome, and necrotizing fasciitis) caused by six distinct M types of GAS seroconverted to the streptococcal cysteine protease. These results demonstrate that this GAS protein is expressed in vivo during the course of human infections and thereby provide additional evidence that the cysteine protease participates in host-pathogen interactions in some patients.
Clinical evaluation of a latex agglutination test for streptococcal pharyngitis (1988)

The accuracy and clinical utility of a latex agglutination test were compared with anaerobic throat culture on selective media for detection of Group A streptococcal pharyngitis in an urban pediatric emergency department. Among 255 symptomatic cases prevalence of positive culture was 29% and antigen test sensitivity was 55%. Among 100 asymptomatic controls prevalence of positive culture was 20% and antigen test sensitivity was 20%. Controls with positive cultures and cases with false negative antigen tests had significantly fewer colonies on culture than cases with true positive antigen tests (P less than 0.01). Symptoms and clinical findings were not associated with antigen test result or number of colonies on culture. Eighty percent of patients with positive cultures received treatment when the antigen test was used as an adjunct to culture, compared with a 57% treatment rate among the subgroup in whom follow-up treatment was attempted based on positive culture results alone (P less than 0.05). We conclude that: (1) the antigen test had lower sensitivity in routine clinical use than previously reported; (2) the high rate of false negative tests may result, in part, from a high proportion of specimens with low colony counts; and (3) the availability of the antigen test as an adjunct to culture significantly increased treatment rates.
Neural-Network-Based Signature Recognition for Harmonic Source Identification (2005)

This paper proposes a neural-network (NN)-based approach to nonintrusive harmonic source identification. In this approach, NNs are trained to extract important features from the input current waveform to uniquely identify various types of devices using their distinct harmonic "signatures". Such automated, noninvasive device identification will be critical in future power-quality monitoring and enhancement systems. Several NN-based classification models including multilayer perceptron (MLP), radial basis function (RBF) network, and support vector machines (SVM) with linear, polynomial, and RBF kernels were developed for signature extraction and device identification. These models were trained and tested using spike train data gathered from the Fourier analysis of the input current waveform in the presence of multiple devices. The performance of these models was compared in terms of their accuracy, generalization ability, and noise tolerance limits. The results showed that MLPs and SVM were both able to determine the presence of devices based on their harmonic signatures with high accuracy. MLP was found to be the best signature identification method because of its low computational requirements and ability to extract the information necessary for highly accurate device identification.
4E-BP1, a multifactor regulated multifunctional protein (2016)

Eukaryotic translation initiation factor 4E (eIF4E)-binding protein 1 (4E-BP1) is a member of a family of translation repressor proteins, and a well-known substrate of mechanistic target of rapamycin (mTOR) signaling pathway. Phosphorylation of 4E-BP1 causes its release from eIF4E to allow cap-dependent translation to proceed. Recently, 4E-BP1 was shown to be phosphorylated by other kinases besides mTOR, and overexpression of 4E-BP1 was found in different human carcinomas. In this review, we summarize the novel findings on mTOR independent 4E-BP1 phosphorylation in carcinomas. The implications of overexpression and possible multi-function of 4E-BP1 are also discussed.
Metabolie Transformation of Colchicine. I. The Oxidative Formation of Products from Colchicine in the Udenfriend System (1973)

Article Metabolie Transformation of Colchicine. I. The Oxidative Formation of Products from Colchicine in the Udenfriend System was published on January 1, 1973 in the journal Biological Chemistry (volume 354, issue 1).
Local Enhancement for Robust Face Detection in Poor SNR Images (2009)

Face detection is an important preprocessing stage for automated facial recognition systems. Imaging in real world environment experience substantial variation in lighting and weather conditions. Surveillance systems are a typical example of real world imaging systems. Face detection is based upon typical haar like patterns appearing in facial images. Performance of even most successful face detection algorithms such as viola’s work, typically fail in poor lighting or in low SNR (Signal to Noise Ratio) images. Therefore subsequent stage of image enhancement after image acquisition is employed to enhance the faces or face patterns in low lighting images.Proposed research work addressed the face detection in automotive environment. Algorithm addressed the situation of low lighting condition and enhanced the images captured in low lighting conditions with lights switched on. Faces of people in automobile cabin go in complete darkness and difficult to enhance. Proposed scheme is based upon local enhancement method and considerable results are achieved.
Expressions of Three Members of ACS Gene Family Induced by Ethephon Relationship of Ethylene Production and Sugar Accumulation in Sugarcane Stalks (2008)

摘要： ACC合酶(ACS)是高等植物乙烯生物合成途径中的限速酶。为了弄清楚ACS基因在甘蔗茎中的表达与乙烯释放量、蔗糖积累的关系，在克隆到甘蔗ACS基因3个成员(Sc-ACS1、Sc-ACS2和Sc-ACS3)的基础上，分别以它们作为探针，Northern杂交检验他们在茎中的时空表达。结果表明，甘蔗生长后期，ACS基因三个成员在茎中不同品种和节间持续表达，Sc-ACS1和Sc-ACS2表达维持较低水平，Sc-ACS3维持较高水平。乙烯利处理明显提高3个基因在茎的未成熟和正在成熟节间的表达，且Sc-ACS1和Sc-ACS2在未成熟节间的表达持续时间长达一个月，而Sc-ACS3在未成熟节间的较高水平表达可持续45 d。该结果与甘蔗节间尤其是未成熟和正在成熟节间的乙烯释放量增加以及糖分积累的效应基本一致。相关分析表明，GT17品种乙烯释放量与蔗糖分在处理后14 d和28 d分别极显著和显著负相关，但B1品种未达显著水平。