gebhart/patrepeval huggingface dataset:
Expediting Prior Approval and Containing Third‐Party Costs for Dental Carea (1992)

Dental insurance carriers frequently require referral of radiographs for determination of prior approval. Radiographs are also often used for obtaining expert opinions before finalizing diagnoses and establishing treatment plans. Traditionally, such referrals have been carried out using the original or duplicate films transmitted through the postal system. Studies concerning alternative communication media for data transmission are presented, namely, the use of switched-digital telephone lines and of electronic mail networks.
Phenolic Ester-Based Initiators for Transition Metal Mediated Living Polymerization (1999)

A range of phenolic esters derived form the esterification of substituted phenols with 2-bromoisobutyryl bromide and 2-chloroisobutyryl chloride have been demonstrated to be effective atom transfer polymerization initiators in conjunction with N-(n-octyl)-2-pyridylmethanimine and copper(I) chloride and bromide. All initiators have been fully characterized. Methoxy, phenyl ether, primary aryl amino, aldehyde, nitro, and benzyl ether functional initiators lead to efficacious atom transfer polymerization without imparting any detrimental effects on the polymerization. Polymerizations have been shown to proceed with excellent first-order kinetics with small amount of termination, as seen by narrow polydispersity indices, often below 1.10. Rates of polymerization are of the of the order of 1 × 10-4 s-1, which gives PMMA of approximately 10 000 g mol-1 after 4 h under typical polymerization conditions. Mn increases linearly with conversion. Polymerization of a range of alkyl methacrylates is demonstrated including ethyl methacrylate, butyl methacrylate (iso- and n-) while tert-butyl methacrylate terminates early in the reaction, with the production of a broad molar mass distribution polymer. Polymerization of styrene proceeds effectively, but at a much slower rate than for MMA. Polymerization of MMA with a chloro functional initiator and CuCl proceeds at approximately half the rate of the bromo analogues with a broadening of PDI. Difunctional and trifunctional initiators are derived from the appropriate polyphenols. The multifunctional initiators are shown to be effective atom transfer initiators to give narrow PDI polymer with controlled Mn. The role of deactivating copper(II) species is further seen by the effect of the polymerization rate in a range of kinetic experiments. Esterified phenols are an extremely versatile and simple route into α-functional polymers via atom transfer polymerization.
Use of data permutation to reduce the peak‐to‐average power ratio of an OFDM signal (2002)

Abstract A set of fixed permutations is used in this paper to reduce the peak‐to‐average power ratio (PAR) of an orthogonal frequency division multiplexing (OFDM) signal. For this technique, K − 1 interleavers are used to produce K − 1 permuted sequences from the same information sequence. The peak powers of the permuted sequences and the original information sequence are computed using K inverse discrete Fourier transforms; the sequence with the lowest PAR is chosen for transmission. Before the optimization process begins the identity of each interleaver is embedded into the data frame as side information (SI). SI which is critical to the receiver operation, is coded using a simple forward error correction code in order to increase its reliability. An adaptive approach is proposed for the reduction of this technique's complexity. Furthermore, theoretical expressions are derived for the complementary cumulative distribution function of the PAR and for the average number of permutations required by the adaptive approach. Computer simulations are performed for finding the PAR reduction capability of several types of interleavers. It is subsequently found that random interleavers and odd–even symmetric interleavers are performing equally well in reducing the PAR. Results are also presented for the out of band radiation and the bit error rate performance of interleaved OFDM (IOFDM) and conventional OFDM in an additive white Gaussian noise channel. IOFDM also has less adjacent channel interference than that of conventional OFDM. Copyright © 2002 John Wiley &amp; Sons, Ltd.
Optimization of Coupled and Decoupled Hydrogen Moderators for a Short-pulse Spallation Source (2003)

Neutronic performance of coupled and decoupled hydrogen moderators for a short pulse spallation source was extensively studied by calculations to seek the optimal parameters, especially the para/ortho hydrogen ratio. A decoupled 100% para-hydrogen moderator gives excellent pulse characteristics at lower energies. Gadolinium (Gd) poisoning is useful to obtain narrower pulses up to about 70 meV at a reasonable intensity penalty. At higher energies, where the pulse tail decay characteristics become most important, the choice of the reflector material and the decoupling energy (E d ) play an important role. One important technical issue is the radiation damage of a Gd-poison and a boron carbide decoupler. We discuss the possibility of using a composite decoupler material such as Cd + In (indium) + Eu (europium). In a coupled moderator a 100% para-hydrogen moderator of a larger volume gives an additional intensity gain over a normal one but the gain is not as large as in a decoupled one.
Mutations in the Substrate Binding Site of Thrombin-activatable Fibrinolysis Inhibitor (TAFI) Alter Its Substrate Specificity (2003)

Thrombin-activable fibrinolysis inhibitor (TAFI) is a zymogen that inhibits the amplification of plasmin production when converted to its active form (TAFIa). TAFI is structurally very similar to pancreatic procarboxypeptidase B. TAFI also shares high homology in zinc binding and catalytic sites with the second basic carboxypeptidase present in plasma, carboxypeptidase N. We investigated the effects of altering residues involved in substrate specificity to understand how they contribute to the enzymatic differences between TAFI and carboxypeptidase N. We expressed wild type TAFI and binding site mutants in 293 cells. Recombinant proteins were purified and characterized for their activation and enzymatic activity as well as functional activity. Although the thrombin/thrombomodulin complex activated all the mutants, carboxypeptidase B activity of the activated mutants against hippuryl-arginine was reduced. Potato carboxypeptidase inhibitor inhibited the residual activity of the mutants. The functional activity of the mutants in a plasma clot lysis assay correlated with their chromogenic activity. The effect of the mutations on other substrates depended on the particular mutation, with some of the mutants possessing more activity against hippuryl-His-leucine than wild type TAFIa. Thus mutations in residues around the substrate binding site of TAFI resulted in altered C-terminal substrate specificity.
Novel defensin subfamily from spinach (Spinacia oleracea) (1998)

Antimicrobial peptides (So-D1-7) were isolated from a crude cell wall preparation from spinach leaves (Spinacia oleracea cv. Matador) and, judged from their amino acid sequences, six of them (So-D2-7) represented a novel structural subfamily of plant defensins (group IV). Group-IV defensins were also functionally distinct from those of groups I-III. They were active at concentrations < 20 microM against Gram-positive (Clavibacter michiganensis) and Gram-negative (Ralstonia solanacearum) bacterial pathogens, as well as against fungi, such as Fusarium culmorum, F. solani, Bipolaris maydis, and Colletotrichum lagenarium. Fungal inhibition occurred without hyphal branching. Group-IV defensins were preferentially distributed in the epidermal cell layer of leaves and in the subepidermal region of stems.
Nuclear Translocation of the p75 Neurotrophin Receptor Cytoplasmic Domain in Response to Neurotrophin Binding (2005)

The intracellular domain of the p75 neurotrophin receptor (p75ICD) can be released by gamma-secretase in response to the previous activation of alpha-secretase by phorbol esters. However, ligand-dependent release of p75ICD has yet to be described. We show here that nerve growth factor can induce the release of p75ICD and facilitate its translocation to the nucleus in a gamma-secretase-dependent manner. This effect was observed in RN22 schwannoma cells cultured under serum-free conditions, as well as in Schwann cells, and it was mimicked by other neurotrophins, such as brain-derived neurotrophic factor or neurotrophin-3. Unlike other known examples of regulated intramembrane proteolysis, ligand-dependent release of p75ICD did not need the previous activation of alpha-secretase. These results suggest that nuclear translocation of p75ICD may represent a novel neurotrophin-mediated signaling pathway.
Three-Dimensional Structure of Human Follicle-Stimulating Hormone (2001)

The crystal structure of a betaThr26Ala mutant of human follicle-stimulating hormone (hFSH) has been determined to 3.0 A resolution. The hFSH mutant was expressed in baculovirus-infected Hi5 insect cells and purified by affinity chromatography, using a betahFSH-specific monoclonal antibody. The betaThr26Ala mutation results in elimination of the betaAsn24 glycosylation site, yielding protein more suitable for crystallization without affecting the receptor binding and signal transduction activity of the glycohormone. The crystal structure has two independent hFSH molecules in the asymmetric unit and a solvent content of about 80%. The alpha- and betasubunits of hFSH have similar folds, consisting of central cystine-knot motifs from which three beta-hairpins extend. The two subunits associate very tightly in a head-to-tail arrangement, forming an elongated, slightly curved structure, similar to that of human chorionic gonadotropin (hCG). The hFSH heterodimers differ only in the conformations of the amino and carboxy termini and the second loop of the beta-subunit (L2beta). Detailed comparison of the structures of hFSH and hCG reveals several differences in the beta-subunits that may be important with respect to receptor binding specificity or signal transduction. These differences include conformational changes and/or differential distributions of polar or charged residues in loops L3beta (hFSH residues 62-73), the cystine noose, or determinant loop (residues 87-94), and the carboxy-terminal loop (residues 94-104). An additional interesting feature of the hFSH structure is an extensive hydrophobic patch in the area formed by loops alphaL1, alphaL3, and betaL2. Glycosylation at alphaAsn52 is well known to be required for full signal transduction activity and heterodimer stability. The structure reveals an intersubunit hydrogen bonding interaction between this carbohydrate and betaTyr58, an indication of a mechanism by which the carbohydrate may stabilize the heterodimer.
Oxidation of the albumin thiol to sulfenic acid and its implications in the intravascular compartment (2009)

Human serum albumin (HSA) is the most abundant protein in the intravascular compartment. It possesses a single thiol, Cys34, which constitutes ~80% of the total thiols in plasma. This thiol is able to scavenge plasma oxidants. A central intermediate in this potential antioxidant activity of human serum albumin is sulfenic acid (HSA-SOH). Work from our laboratories has demonstrated the formation of a relatively stable sulfenic acid in albumin through complementary spectrophotometric and mass spectrometric approaches. Recently, we have been able to obtain quantitative data that allowed us to measure the rate constants of sulfenic acid reactions with molecules of analytical and biological interest. Kinetic considerations led us to conclude that the most likely fate for sulfenic acid formed in the plasma environment is the reaction with low molecular weight thiols to form mixed disulfides, a reversible modification that is actually observed in ~25% of circulating albumin. Another possible fate for sulfenic acid is further oxidation to sulfinic and sulfonic acids. These irreversible modifications are also detected in the circulation. Oxidized forms of albumin are increased in different pathophysiological conditions and sulfenic acid lies in a mechanistic junction, relating oxidizing species to final thiol oxidation products.
The Personal Injury Exclusion: Congress Gets Physical but Leaves the Exclusion Emotionally Distressed (1997)

Shortly after the income tax became a permanent part of the federal tax landscape in 1913, Congress added a provision to the Internal Revenue Code allowing taxpayers to exclude from income “any damages” received “on account of personal injury or sickness.” Since its enactment, § 104(a)(2) has been the subject of intense scrutiny and constant litigation. Part II of this article reviews the history of the personal injury exclusion. It finds that, at its inception, the exclusion actually constituted two separate exclusions: a statutory exclusion for physical injuries and a nonstatutory exclusion for nonphysical injuries. It examines the policy rationale for both exclusions and determines that each resulted from a questionable use of precedent by early tax policymakers. Eventually, the two exclusions were merged, first by the courts and then by Congress, as a result of the 1989 amendment. This historical review leads to two conclusions. First, if Congress, by amending § 104(a)(2), is stating that the exclusion never was intended to apply to nonphysical injuries, despite the 1989 amendment, then Congress’s position is correct and both the Service and the courts should reject the extension of § 104(a)(2) to nonphysical injuries in all preenactment cases. Second, and more importantly, history reveals that the original justification for the physical injury exclusion was seriously flawed and should have been rejected by both Congress and the courts. Part III then examines amended § 104(a)(2) and questions whether Congress’s decision to draw a line between physical and nonphysical injuries and sickness can be applied sensibly. Congress’s attempt to provide a limited exclusion for the nonphysical injury of emotional distress creates serious interpretive difficulties that courts are likely to wrestle with in the near future. Part IV asks the question Did Congress go far enough? It reviews human capital theory and its recent metamorphosis and suggests that this theory remains a suspect foundation for the personal injury exclusion. Consequently, Part IV concludes that Congress was wrong; the only appropriate congressional action with respect to § 104(a)(2) was repeal.
Effect of Chronic Staphylococcal Mastitis on Mitogenic Responses of Bovine Lymphocytes (1985)

We compared mitogenic responses of milk and peripheral blood lymphocytes from nonmastitic (control) cows and cows with experimentally induced staphylococcal mastitis in one gland. Milk lymphocytes from infected glands were essentially unresponsive to Concanavalin A, phytohemagglutinin-P, and pokeweed mitogen. Proliferative responses of milk lymphocytes from uninfected glands of infected cows were not as depressed as those from infected glands but were significantly less than those of milk lymphocytes from control cows. Proliferative responses of peripheral blood lymphocytes from control and infected cows to Concanavalin A and phytohemagglutinin-P were similar; however, peripheral blood lymphocytes from infected cows responded in reduced fashion to pokeweed mitogen compared with peripheral blood lymphocytes from control animals. These observations demonstrate that in vitro lymphocyte blastogenesis is markedly depressed during infection, suggesting that in vivo lymphocyte function is compromised, possibly contributing to the chronicity of staphylococcal mastitis.
Retrovirus-mediated gene transfer of human adenosine deaminase: expression of functional enzyme in murine hematopoietic stem cells in vivo. (1987)

Simplified Moloney murine leukemia virus-based recombinant retrovirus vectors have been constructed which transduce human adenosine deaminase (ADA) cDNA. ADA transcription is under the control of the constitutive promoter for the human X chromosome phosphoglycerate kinase (pgk) gene. In these simplified vectors, dominant selectable markers are not included and selection is dependent on overproduction of functional ADA enzyme. Primary murine hematopoietic cells were infected with helper-free recombinant ADA virus generated from Psi-2 packaging cells. Protein analysis revealed that human ADA enzyme was expressed in progenitor-derived hematopoietic colonies in vitro and CFU-S-derived spleen colonies in vivo. Enzyme expression was dependent on transcription from the pgk promoter. ADA expression in primary murine hematopoietic cells directed by the internal promoter was not adversely affected by the presence of the Moloney virus long terminal repeat enhancer sequence. Use of these vectors allows systematic evaluation of the effects of specific sequences in recombinant retrovirus vectors on expression in primary murine hematopoietic cells in vivo.
Electronic effects of Ziegler-Natta polymerization of propylene and ethylene using soluble metallocene catalysts (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectronic effects of Ziegler-Natta polymerization of propylene and ethylene using soluble metallocene catalystsIk Mo Lee, William J. Gauthier, JoAnne M. Ball, Bhagavathi Iyengar, and Scott CollinsCite this: Organometallics 1992, 11, 6, 2115–2122Publication Date (Print):June 1, 1992Publication History Published online1 May 2002Published inissue 1 June 1992https://pubs.acs.org/doi/10.1021/om00042a027https://doi.org/10.1021/om00042a027research-articleACS PublicationsRequest reuse permissionsArticle Views1168Altmetric-Citations190LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
2',5'-Oligoadenylate:antisense chimeras. Synthesis and properties (1993)

We have synthesized a novel bioconjugate which joins an antisense oligonucleotide to a unique and potent inhibitor of translation,pn5'A2'(p5'A2')mp5'A(2-5A). Two residues of 4-hydroxybutyl phosphate were employed as linkers to attach the 2',5'-oligoadenylate moiety through its 2'-terminus to the 5'-terminus of the chosen antisense sequence, (dT)20. The syntheses were carried on a solid support according to the phosphite triester method of DNA synthesis (Letsinger, R.L., Lunsford, W.B. (1976) J. Am. Chem. Soc. 98, 3655-3661; Beaucage, S.L., and Caruthers, M.H. (1981) Tetrahedron Lett. 22, 1859-1862). The generated 2-5A antisense chimeras retained both the ability of the 2-5A molecule to activate the 2-5A-dependent RNase as well as the ability of the oligo(dT) moiety to hybridize to the complementary poly(A). Moreover, the chimera, when annealed to its target nucleic acid sequence, was still effectively bound to the 2-5A-dependent nuclease. The methodology described represents a new approach to the selective modulation of mRNA expression.
Social Network Analysis (1994)

Social network analysis is used widely in the social and behavioral sciences, as well as in economics, marketing, and industrial engineering. The social network perspective focuses on relationships among social entities and is an important addition to standard social and behavioral research, which is primarily concerned with attributes of the social units. Social Network Analysis: Methods and Applications reviews and discusses methods for the analysis of social networks with a focus on applications of these methods to many substantive examples. It is a reference book that can be used by those who want a comprehensive review of network methods, or by researchers who have gathered network data and want to find the most appropriate method by which to analyze it. It is also intended for use as a textbook as it is the first book to provide comprehensive coverage of the methodology and applications of the field.
Synthesis and biological evaluation of prodrugs of zidovudine (1990)

A series of prodrugs of zidovudine (AZT) has been synthesized in an effort to enhance the uptake of the prodrugs by the HIV-1 infected cells and to increase the plasma half-life of AZT. The 5'-OH function of AZT was esterified with various acids in the presence of DCC and 4-(dimethylamino)pyridine (DMAP). The prodrug moieties included (a) morpholine and N-phenylpiperazine-1-acetic acid, (b) 1,4-dihydro-1-methyl-3-nicotinic acid, (c) retinoic acid, and (d) certain amino acids. The anti-HIV-1 activity of the esters was determined in peripheral blood lymphocytes. The IC50 for AZT in this system was 0.12 microM whereas for prodrugs it ranged from 0.05 to 0.2 microM. The prodrugs were generally less cytotoxic than AZT except the retinoic acid ester. In vitro hydrolysis of the various esters in human plasma indicated that these agents were relatively stable toward plasma esterases with t1/2 ranging from 10 to 240 min. Drug uptake studies in H9 cells with radiolabeled analogues demonstrated that the retinoic acid ester achieved approximately 4-fold higher intracellular concentration than [3H]AZT. However, 1,4-dihydro-1-methyl-3-[(pyridylcarbonyl)oxy] ester (5) was the most active agent of this series and had a higher therapeutic index than AZT.
Basal cytokeratins and their relationship to the cellular origin and functional classification of breast cancer (2005)

Recent publications have classified breast cancers on the basis of expression of cytokeratin-5 and -17 at the RNA and protein levels, and demonstrated the importance of these markers in defining sporadic tumours with bad prognosis and an association with BRCA1-related breast cancers. These important observations using different technology platforms produce a new functional classification of breast carcinoma. However, it is important in developing hypotheses about the pathogenesis of this tumour type to review the nomenclature that is being used to emphasize potential confusion between terminology that defines clinical subgroups and markers of cell lineage. This article reviews the lineages in the normal breast in relation to what have become known as the 'basal-like' carcinomas.