gebhart/patrepeval huggingface dataset:
Kinetics and thermodynamics of the complex-formation of nickel(II) and cobalt(II) ions with ammonia and pyridine-2-azodimethylaniline in water, at pressures from 1 bar to 2 kbar (1972)

Thermodynamic and kinetic measurements have been made at a series of pressures from 1 bar to 2 kbar on the reactions of nickel(II) and cobalt(II) ions with ammonia and with the large bidentate ligand PADA (pyridine-2-azodimethylaniline) in aqueous solution. The apparatus consisted of a high-pressure cell and a laser temperature-jump apparatus with spectrophotometric detection. A method is described for determining equilibrium constants at high pressures from the amplitude of the optical-density change on reaction. The values found for ΔV≠ are all in the region of +5 to +8 cm3 mol–1. The results are consistent with the interpretation that the transition state involves considerable stretching of the cation-water bond, as postulated in the Eigen-Wilkins mechanism.
ChemInform Abstract: Recent Developments in the Use of N‐Phthaloyl‐Amino Acid Derivatives in Synthesis (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
The c-Abl tyrosine kinase regulates actin remodeling at the immune synapse (2008)

Abstract Actin dynamics during T-cell activation are controlled by the coordinate action of multiple actin regulatory proteins, functioning downstream of a complex network of kinases and other signaling molecules. The c-Abl nonreceptor tyrosine kinase regulates actin responses in nonhematopoietic cells, but its function in T cells is poorly understood. Using kinase inhibitors, RNAi, and conditional knockout mice, we investigated the role of c-Abl in controlling the T-cell actin response. We find that c-Abl is required for normal actin polymerization and lamellipodial spreading at the immune synapse, and for downstream events leading to efficient interleukin-2 production. c-Abl also plays a key role in signaling chemokine-induced T-cell migration. c-Abl is required for the appropriate function of 2 proteins known to be important for controlling actin responses to T-cell receptor (TCR) engagement, the actin-stabilizing adapter protein HS1, and the Rac1-dependent actin polymerizing protein WAVE2. c-Abl binds to phospho-HS1 via its SH2 domains and is required for full tyrosine phosphorylation of HS1 during T-cell activation. In addition, c-Abl is required for normal localization of WAVE2 to the immune synapse (IS). These studies identify c-Abl as a key player in the signaling cascade, leading to actin reorganization during T-cell activation.
The Conformational Properties of Epothilone. (2000)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionThe Conformational Properties of Epothilone.Richard E. Taylor and Jaroslav ZajicekCite this: J. Org. Chem. 2000, 65, 17, 5449–5450Publication Date (Web):July 6, 2000Publication History Published online6 July 2000Published inissue 1 August 2000https://pubs.acs.org/doi/10.1021/jo0040240https://doi.org/10.1021/jo0040240correctionACS PublicationsCopyright © 2000 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views282Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (27 KB) Get e-Alertsclose Get e-Alerts
Evaluation of an Attenuated Vesicular Stomatitis Virus Vector Expressing Interferon-β for Use in Malignant Pleural Mesothelioma: Heterogeneity in Interferon Responsiveness Defines Potential Efficacy (2009)

Abstract Vesicular stomatitis virus (VSV) has shown promise as an oncolytic agent, although unmodified VSV can be neurotoxic. To avoid toxicity, a vector was created by introducing the interferon-beta (IFN-beta) gene (VSV.IFN-beta). We conducted this study to determine the ability of VSV.IFN-beta to lyse human cancer (mesothelioma) cells and to evaluate the potential of this recombinant virus for clinical translation. Four normal human mesothelial and 12 mesothelioma cell lines were tested for their susceptibility to VSV vectors in vitro. VSV.hIFN-beta did not cause cytotoxicity in any normal lines. Only 4 of 12 lines were effectively lysed by VSV.hIFN-beta. In the eight resistant lines, pretreatment with IFN-beta prevented lysis of cells by VSV.GFP, and VSV infection or addition of IFN-beta protein resulted in the upregulation of double-stranded RNA-dependent protein kinase (PKR), myxovirus resistance A (MxA), and 2',5'-oligo-adenylate-synthetase (2'5'-OAS) mRNA. In the susceptible lines, there was no protection by pretreatment with IFN-beta protein and no IFN- or VSV-induced changes in PKR, MxA, and 2'5'-OAS mRNA. This complete lack of IFN responsiveness could be explained by marked downregulation of interferon alpha receptors (IFNARs), p48, and PKR in both the mesothelioma cell lines and primary tumor biopsies screened. Presence of p48 in three tumor samples predicted responsiveness to IFN. Our data indicate that many mesothelioma tumors have partially intact IFN pathways that may affect the efficacy of oncolytic virotherapy. However, it may be feasible to prescreen individual susceptibility to VSV.IFN-beta by immunostaining for the presence of p48 protein.
Studies on the dignosis of depth of invasion in rectal cancer using intrarectal echography, CT and MRI. (1988)

直腸癌の術前検査として直腸内超音波法,computed tomography(CT),およびmagnetic resonance imaging(MRI)を施行し,各診断法による壁深達度診断能について検討した.対象は1981年6月～1987年7月の直腸癌症例163例で,直腸内超音波法を163例に,CTを121例に,MRIを52例に行った.直腸内超音波法では深達度を5群に分類可能であり,M'群:85.7%,SM'群:71.4%,PM'群:60.0%,SS'～S'・A'1～A'2群:90.2%,Si'・Ai'群:64.3%の正診が得られた.CTおよびMRIにより3群に分類した深達度診断では,I群:CT66.7%,MRI80.0%,II群:CT81.5%,MRI91.7%,III群:CT32.1%,MRI50.0%の正診が得られた.詳細な壁深達度診断では直腸内超音波法がもっとも優れた診断法であり,他臓器浸潤の有無に関する診断ではMRIによる検索がもっとも有効であった.各診断法の今後の発達により,より正確な術前診断が可能になると期待される.
Periodontal Regeneration – Intrabony Defects: A Systematic Review From the AAP Regeneration Workshop (2015)

1) Biologics (enamel matrix derivative and recombinant human platelet-derived growth factor-BB plus β-tricalcium phosphate) are generally comparable with demineralized freeze-dried bone allograft and GTR and superior to open flap debridement procedures in improving clinical parameters in the treatment of intrabony defects. 2) Histologic evidence of regeneration has been demonstrated with laser therapy; however, data are limited on clinical predictability and effectiveness. 3) Clinical outcomes appear most appreciably influenced by patient behaviors and surgical approach rather than by tooth and defect characteristics. 4) Long-term studies indicate that improvements in clinical parameters are maintainable up to 10 years, even in severely compromised teeth, consistent with a favorable/good long-term prognosis.
Performance adaptive training control strategy for recovering wrist movements in stroke patients: a preliminary, feasibility study (2009)

The positive outcomes of the preliminary tests motivate the planning of a clinical trial and provide experimental evidence for defining appropriate inclusion/exclusion criteria.
Preparation and Study of 1,8-Di(pyrid-2′-yl)carbazoles (2008)

A series of three derivatives of 1,8-di(pyrid-2'-yl)carbazole were prepared by Stille-type coupling of 2-(tri-n-butylstannyl)pyridine with the appropriate 1,8-dibromocarbazole. The carbazoles were prepared by appropriate substitution methodologies on the parent carbazole or by palladium-catalyzed cyclization of di-(p-tolyl)amine to provide the carbazole ring system. An X-ray structure of the di-tert-butyl derivative confirmed that both pyridyl groups were oriented for favorable intramolecular H-bonding to the central N-H. Two orientations of the molecule were found in the unit cell and this observation was corroborated by two N-H stretching bands in the solid state IR. Substitution of N-H by N-D led to increased emission intensity through diminished intramolecular deactivation of the excited state. The di-tert-butyl derivative formed a tridentate complex with Pd(II), which showed a red-shifted band attributed to an intraligand charge transfer state.
Degradation of specificity in cytolytic T lymphocyte clones. The separate YAC-1-type (NK-like) and P815-type broad specificity killing patterns are both restricted to the larger cells within a clone but may be expressed independently in clones from different mouse strains. (1986)

Ly-2+ T cells cultured at limiting dilution with concanavalin A and irradiated spleen filler cells develop into clones of Ly-2+ cytotoxic T lymphocytes (CTL), which although initially specific, lyse a wide range of target cells by days 8 to 9. This anomalous nonspecific killing is now shown to be a function of the largest cells within the clone, with the majority of CTL behaving normally. Cold-target inhibition experiments demonstrate that two distinct recognition systems determine the broad-range killing, one being typified by a high affinity for P815 tumor cells, the other being typified by an affinity for YAC-1 tumor cells. Mouse strains differ in the probability that CTL in culture will demonstrate one or another of these killing patterns; some develop both, some one, and some neither. When both killing patterns develop, as in cultures of Ly-2+ cells from CBA mice, both are expressed in the same clone. High natural killer (NK) cell strains are those most likely to develop CTL clones with an ability to lyse the NK target YAC-1. The results have implications for the relationship of NK cells to T cells. They also suggest ways of avoiding the problem of anomalous T cell killing.
SNP Genotyping by Multiplexed Solid-Phase Amplification and Fluorescent Minisequencing (2001)

The emerging role of single-nucleotide polymorphisms (SNPs) in clinical association and pharmacogenetic studies has created a need for high-throughput genotyping technologies. We describe a novel method for multiplexed genotyping of SNPs that employs PCR amplification on microspheres. Oligonucleotide PCR primers were designed for each polymorphic locus such that one of the primers contained a recognition site for BbvI (a type IIS restriction enzyme), followed by 11 nucleotides of locus-specific sequence, which reside immediately upstream of the polymorphic site. Following amplification, this configuration allows for any SNP to be exposed by BbvI digestion and interrogated via primer extension, four-color minisequencing. Primers containing 5' acrylamide groups were attached covalently to the solid support through copolymerization into acrylamide beads. Highly multiplexed solid-phase amplification using human genomic DNA was demonstrated with 57 beads in a single reaction. Multiplexed amplification and minisequencing reactions using bead sets representing eight polymorphic loci were carried out with genomic DNA from eight individuals. Sixty-three of 64 genotypes were accurately determined by this method when compared to genotypes determined by restriction-enzyme digestion of PCR products. This method provides an accurate, robust approach toward multiplexed genotyping that may facilitate the use of SNPs in such diverse applications as pharmacogenetics and genome-wide association studies for complex genetic diseases.
Nicotinic Acetylcholine Receptors as Drug Targets (2004)

While it has long been documented that nicotine contained in tobacco leaves gives rise to major public health problems it has also been observed that this alkaloid can have beneficial effects. However, it is only with the identification of a family of genes coding for the neuronal nicotinic acetylcholine receptors and increased knowledge of their expression and function in the central nervous system that these receptors have received attention concerning their potential as drug targets. In light of the latest findings about nicotinic acetylcholine receptors and their involvement in disease states we review the possibility to design new drugs targeted to these ligand-gated channels. Beneficial and possible undesirable actions of agonists, antagonists and allosteric modulators are discussed and placed in perspective of our most recent knowledge.
Human ATP-binding cassette transporter 1 (ABC1): Genomic organization and identification of the genetic defect in the original Tangier disease kindred (1999)

Tangier disease is characterized by low serum high density lipoproteins and a biochemical defect in the cellular efflux of lipids to high density lipoproteins. ABC1, a member of the ATP-binding cassette family, recently has been identified as the defective gene in Tangier disease. We report here the organization of the human ABC1 gene and the identification of a mutation in the ABC1 gene from the original Tangier disease kindred. The organization of the human ABC1 gene is similar to that of the mouse ABC1 gene and other related ABC genes. The ABC1 gene contains 49 exons that range in size from 33 to 249 bp and is over 70 kb in length. Sequence analysis of the ABC1 gene revealed that the proband for Tangier disease was homozygous for a deletion of nucleotides 3283 and 3284 (TC) in exon 22. The deletion results in a frameshift mutation and a premature stop codon starting at nucleotide 3375. The product is predicted to encode a nonfunctional protein of 1,084 aa, which is approximately half the size of the full-length ABC1 protein. The loss of a Mnl1 restriction site, which results from the deletion, was used to establish the genotype of the rest of the kindred. In summary, we report on the genomic organization of the human ABC1 gene and identify a frameshift mutation in the ABC1 gene of the index case of Tangier disease. These results will be useful in the future characterization of the structure and function of the ABC1 gene and the analysis of additional ABC1 mutations in patients with Tangier disease.
6doku: Towards Secure Over-the-Air Preloading of 6LoWPAN Nodes using PHY Key Generation (2015)

Currently, adding a new node to an existing 6Lo-WPAN network is tedious. The user has to plug a cable into each new node so as to preload it with cryptographic material, as well as other configuration settings. This approach not only lacks usability and scalability, but also incurs higher per unit costs due to USB ports. To facilitate preloading, Wilhelm et al. proposed "SUDOKU" - a scheme for preloading nodes wirelessly. SUDOKU incorporates PHY key generation and Diffie-Hellman key exchange. In this paper, we propose a streamlined and more practical version of SUDOKU entitled "6DOKU". 6DOKU dispenses with Diffie-Hellman key exchange, which saves resources. Furthermore, 6DOKU provides the user with a mobile preloading tool, whereas SUDOKU requires the user to preload nodes in an isolated area before installation. 6DOKU not only improves on SUDOKU, but is competitive among other wireless preloading schemes, too. We prototyped 6DOKU's preloading tool as an Android app and give first experimental results.
Effects of dihydrolipoic acid on peptide methionine sulfoxide reductase. Implications for antioxidant drugs. (1998)

By showing that dihydrolipoic acid (DHLA) reactivates oxidatively damaged alpha-1 antiprotease (alpha 1-AP), a new antioxidant property of DHLA is described. For the first time, it is shown that a drug is able to reverse oxidative damage of physiologically essential macromolecules. Until now, only antioxidant properties have been reported that prevent oxidative stress. Repair of oxidized alpha 1-AP is catalysed by peptide methionine sulfoxide reductase (PMSR, EC 1.8.4.6). It is found that DHLA acts as a reducing cofactor for PMSR. Oxidized alpha 1-AP has been implicated in the etiology of certain lung diseases. Generally, by stimulating PMSR, DHLA may exert a curative effect in diseases accompanied by oxidative stress.
Temporal Analysis of Andes Virus and Sin Nombre Virus Infections of Syrian Hamsters (2007)

Andes virus (ANDV) and Sin Nombre virus (SNV) are rodent-borne hantaviruses that cause a highly lethal hemorrhagic fever in humans known as hantavirus pulmonary syndrome (HPS). There are no vaccines or specific drugs to prevent or treat HPS, and the pathogenesis is not understood. Syrian hamsters infected with ANDV, but not SNV, develop a highly lethal disease that closely resembles HPS in humans. Here, we performed a temporal pathogenesis study comparing ANDV and SNV infections in hamsters. SNV was nonpathogenic and viremia was not detected despite the fact that all animals were infected. ANDV was uniformly lethal with a mean time to death of 11 days. The first pathology detected was lymphocyte apoptosis starting on day 4. Animals were viremic and viral antigen was first observed in multiple organs by days 6 and 8, respectively. Levels of infectious virus in the blood increased 4 to 5 logs between days 6 and 8. Pulmonary edema was first detected ultrastructurally on day 6. Ultrastructural analysis of lung tissues revealed the presence of large inclusion bodies and substantial numbers of vacuoles within infected endothelial cells. Paraendothelial gaps were not observed, suggesting that fluid leakage was transcellular and directly attributable to infecting virus. Taken together, these data imply that HPS treatment strategies aimed at preventing virus replication and dissemination will have the greatest probability of success if administered before the viremic phase; however, because vascular leakage is associated with infected endothelial cells, a therapeutic strategy targeting viral replication might be effective even at later times (e.g., after disease onset).
Functional dissociation of CD8 α’s Ig homologue and connecting peptide domains (1996)

The contribution of the CD8 alpha.IgV homologue domain to class I MHC binding was evaluated using a series of chimeric human CD8 alpha:Fc polypeptides incorporating alternative CD8 alpha extracellular domain components. Using a nonisotopic cellfree physical binding assay, those Fc chimeras encompassing the CD8 alpha.IgV homologue domain only (dissociated from the 48-amino acid CD8 alpha connecting peptide) were shown to retain the capacity of the complete CD8 alpha extracellular domain to bind to a recombinant soluble class I MHC alpha 3 domain unit or to intact class I MHC. The specificity of the CD8 alpha:class I MHC alpha 3 domain interaction was verified by mAb and soluble polypeptide blocking experiments. Furthermore, co-precipitation of an Fc chimera incorporating only the CD8 alpha.IgV homologue domain and a recombinant soluble class I MHC alpha 3 domain unit was accomplished. In addition, a glycosylphosphatidylinositol (GPI)-modified variant of the CD8 alpha.IgV homologue domain was generated via chimerization with the GPI signal sequence from decay-accelerating factor. GPI anchorage for this truncated CD8 alpha polypeptide was verified, and its capacity to promote intercellular adhesion through class I MHC binding was shown in a cell:cell binding assay. The findings indicate that the CD8 alpha.IgV homologue domain acts as an independent structural unit when dissociated from the CD8 alpha connecting peptide, and in so doing retains class I MHC binding capacity. This further establishes the principle that Ig superfamily domains from receptor:counter-receptor pairs can interact with each other as isolated units, providing an experimental path for tailoring therapeutically useful IgSF protein derivatives.
Using Selection to Identify and Chemical Microarray to Study the RNA Internal Loops Recognized by 6′‐N‐Acylated Kanamycin A (2007)

Herein, we describe our initial steps towards identifying the RNA secondary structure motifs that are recognized by small molecules. We selected members of an RNA 3x3 internal loop motif library that bind kanamycin A, an RNA-binding aminoglycoside antibiotic, by using only one round of selection. A small internal-loop library was chosen because members are likely to be present in other larger, biologically relevant RNAs. We have identified several internal loops of various size and base composition that kanamycin A prefers to bind. The highest affinity structures are two 5'-UU/3'-CU 2x2 internal loops closed by AU pairs. Binding is specific for the selected internal loops with the highest affinities, since binding to the RNA cassette used to display the library or to DNA is >150-fold weaker. Enzymatic mapping experiments also confirm binding of kanamycin A to the internal loops. This method lays the foundation for finding RNA secondary structure elements that bind small molecules and for interrogating factors affecting RNA-ligand interactions. Information from these and subsequent studies will: 1) facilitate the rational and modular design of drugs or probes that bind target RNAs with high affinity, provided the secondary structure of the target is known and 2) give insight into the potential bystander RNAs that aminoglycosides bind.
Subtyping of hepatitis C virus isolates by a line probe assay using hybridization (1995)

A reverse hybridization test (Inno-LiPA HCV; Innogenetics, N.V., Zwijnaarde, Belgium) was used for typing hepatitis C virus. All 38 samples, typed by PCR with primers from core and NS5 genes, were also genotyped by this test. Of the samples, 33 (87%) had the same subtypes by both assays. The correlations between PCR and Inno-LiPA for individual types were 77% for type I (1a), 90% for type II (1b), 100% for type III (2a), 100% for type IV (2b), and 100% for type V (3a). One of the type III (2a) samples also reacted with type I (1a) probes in the Inno-LiPA test.
High-Dose Intravenous Methylprednisolone for Hantavirus Cardiopulmonary Syndrome in Chile: A Double-Blind, Randomized Controlled Clinical Trial (2013)

NCT00128180.
[Level of thiamine diphosphate in the liver of tumor-bearing animals kept on a diet including an excessive amount of vitamin B 1]. (1986)

In mice bearing solid Ehrlich's carcinoma, food consumption did not differ from that in intact animals throughout the whole experiment. In mice with Ehrlich's ascites carcinoma and in rats bearing Walker's carcinosarcoma 256, the daily feed consumption decreased 2-fold by the terminal period of tumor development. In rats, the amount of vitamin B1, calculated per unit of the feed consumed, exceeded the daily requirements of vitamin B1. The content of thiamine diphosphate in the liver of tumor-bearing animals was 35% as reduced whatever the method of inoculation and site of tumor development (under the skin or inside the abdominal cavity). In rats with Walker's carcinosarcoma 256, transketolase activity was 17% as lowered (P less than 0.01). In mice with Ehrlich's carcinoma, the activity of the enzyme was 21% as decreased (P less than 0.01; an ascitic variety) or 17% as decreased (P less than 0.01; subcutaneously inoculated tumor).