gebhart/patrepeval huggingface dataset:
Inhibition of Human Telomerase by a G-Quadruplex-Interactive Compound (1997)

ADVERTISEMENT RETURN TO ISSUEPREVLetterNEXTInhibition of Human Telomerase by a G-Quadruplex-Interactive CompoundDaekyu Sun, Brian Thompson, Brian E. Cathers, Miguel Salazar, Sean M. Kerwin, John O. Trent, Terence C. Jenkins, Stephen Neidle, and Laurence H. HurleyView Author Information Institute for Drug Development, 14960 Omicron Drive, San Antonio, Texas 78245-3217, College of Pharmacy, The University of Texas at Austin, Austin, Texas 78712-1074, and CRC Biomolecular Structure Unit, The Institute of Cancer Research, Cotswold Road, Sutton, Surrey SM2 5NG, United Kingdom Cite this: J. Med. Chem. 1997, 40, 14, 2113–2116Publication Date (Web):July 4, 1997Publication History Received25 March 1997Published online4 July 1997Published inissue 1 July 1997https://pubs.acs.org/doi/10.1021/jm970199zhttps://doi.org/10.1021/jm970199zrapid-communicationACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views5835Altmetric-Citations687LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Chemical structure,G-Quadruplex,Genetics,Inhibition,Peptides and proteins Get e-Alerts
Vehicle-to-vehicle safety messaging in DSRC (2004)

This paper studies the design of layer-2 protocols for a vehicle to send safety messages to other vehicles. The target is to send vehicle safety messages with high reliability and low delay. The communication is one-to-many, local, and geo-significant. The vehicular communication network is ad-hoc, highly mobile, and with large numbers of contending nodes. The messages are very short, have a brief useful lifetime, but must be received with high probability. For this environment, this paper explores the efficacy of rapid repetition of broadcast messages. This paper proposes several random access protocols for medium access control. The protocols are compatible with the Dedicated Short Range Communications (DSRC) multi-channel architecture. Analytical bounds on performance of the proposed protocols are derived. Simulations are conducted to assess the reception reliability and channel usage of the protocols. The sensitivity of the protocol performance is evaluated under various offered traffic and vehicular traffic flows. The results show our approach is feasible for vehicle safety messages in DSRC.
Transferring international politics in education to national school systems: case studies from Ukraine and Romania on the implementation process of a Council of Europe material (2009)

The paper consists of the guest editorial of issue number 21 of the online journal TiBi (Trends in Bildung International). The editorial gives an overview on the two studies which are presented by the journal. Moreover the context of the studies is explained. This context consists of the policy on education for democratic citizenship by the Council of Europe and in a narrower sense in the Council of Europe's intention to introduce quality assurance of education for democratic citizenship in European schools. The two studies report on approaches to implementation in two different countries. They follow up an earlier comparative study of ten countries. While this more comprehensive study analyses the preconditions and opportunities for implementing a material of the Council of Europe, TiBi presents studies on countries, where real implementation took place. (DIPF/Orig.)
How Does the Plethysmogram Derived from the Pulse Oximeter Relate to Arterial Blood Pressure in Coronary Artery Bypass Graft Patients? (2001)

Statistical comparison was made between measured blood pressure (BP) from arterial line and calculated BP derived from ear pulse oximeter waveform in 10 patients undergoing coronary artery bypass graft surgery. Using 62,077 paired readings, the mean difference for systolic BP, mean BP, and pulse pressure between the 2 methods was -2.6, -1.88, and -1.28 mm Hg, respectively.
HLA class II transgenic mice: models of the human CD4+ T‐cell immune response (1999)

This review examines the field of current HLA class II transgenic mouse models and the individual approaches applied in production of these mice. The majority of these mice have been created with the objective of obtaining a disease model with clinical features mimicking human autoimmune disease. The development process of a different type of HLA class II transgenic mice, which are designed to function as a substitute for a normal human immune system in studies of human autoantigens, is described. Several HLA-DR4 transgenic lines with normally expressed HLA-DR4 molecules have been produced. To obtain adequate positive selection of the HLA-DR4-restricted CD4+ T-cell repertoire in these mice it is essential both to introduce a human CD4 transgene, and to delete the murine major histocompatibility complex (MHC) class II molecules. These HLA-DR4 transgenic mice have been used to determine the immunogenic CD4+ T-cell epitopes of several human autoantigenic proteins.
Cutting Edge: IL-17F, a Novel Cytokine Selectively Expressed in Activated T Cells and Monocytes, Regulates Angiogenesis and Endothelial Cell Cytokine Production (2001)

A novel secreted cytokine, termed IL-17F, was cloned using nested RACE PCR. This cytokine bears homology to IL-17. IL-17F was expressed only in activated CD4(+) T cells and activated monocytes. Recombinant human IL-17F did not stimulate the proliferation of hematopoietic progenitors or the migration of mature leukocytes. However, it markedly inhibited the angiogenesis of human endothelial cells and induced endothelial cells to produce IL-2, TGF-beta, and monocyte chemoattractant protein-1.
Effects of nitrogen fertilizer and elevated CO<sub>2</sub> on dry matter production and yield of rice cultivars (1970)

A pot experiment was conducted at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during the year 2003 to find out the dry matter production and yield of rice cultivars under different nitrogen levels and growing conditions. Thirty-day old single seedlings were transplanted in pot and were placed in 3 growing conditions, such as i) Open top chamber (OTC) with elevated CO2 (570 ± 50 ppm), ii) OTC with ambient CO2 (360 ± 50 ppm), and iii) open field condition. The three nitrogen levels used were, i) control, ii) optimum dose, and iii) supra optimum dose. Three rice cultivars used in the experiment were, i) BRRI dhan 39, ii) Khashkani, and iii) Shakkarkhora. Rice yield and dry matter production respond significantly to different environments. Increasing atmospheric CO2 increased grain yield. Stem dry weight, leaf dry weight, leaf sheath dry weight and root dry weight were increased in elevated CO2 than ambient CO2 and field condition. BRRI dhan 39 gave highest yield (50.82 g/plant) at supra optimum N level in elevated CO2. Local variety gave similar result under elevated CO2 in optimum and supra optimum N levels. The lowest yield (15.09 g/plant) was produced by Shakkorkhora in field condition with no nitrogen application. Key Words: Nitrogen; elevated CO2; yield; dry matter.DOI: 10.3329/bjar.v34i2.5804Bangladesh J. Agril. Res. 34(2): 313-322, June 2009
Comparison of Antiadhesive Treatments using an Objective Rat Model (1999)

Intraperitoneal adhesions are a significant problem (increased morbidity, mortality, and cost) for patients undergoing abdominal procedures. Although a variety of approaches (e.g., fibrinolytic agents, anti-inflammatory drugs, or barrier/separation methods) have been used with some success in preventing adhesions, a comparison of these different modalities has yet to be performed in a model that objectively measures intraperitoneal adhesion formation. Our objectives were to establish an objective, reproducible model of intraperitoneal adhesion formation and to establish efficacy of different treatment modalities in decreasing the strength and extent of intraperitoneal adhesions. In this two-part study, a rat model establishing an objective measure of both the strength and extent of intraperitoneal adhesions was used to compare different treatment modalities. Fibrinolytic agents [recombinant tissue plasminogen activator (rtPA), streptokinase, and urokinase], anti-inflammatory drugs (dexamethasone and tolmetin sodium), and barrier methods [sodium carboxymethylcellulose (CMC), and sodium hyaluronate] and a control group were compared in the first phase. In the second phase, the two most successful agents (rtPA, CMC) were compared both alone and in combination against a commercially available barrier agent (Seprafilm) and a control group. In the first phase of the study, rtPA was the only agent that had a statistically significant effect in decreasing the strength of adhesions. CMC was the only agent that demonstrated a decrease in the extent of adhesions, and the difference tended toward significance. In the second phase, the combination of rtPA and CMC showed a significant decrease in both the strength and extent of adhesions when compared with those of the control group. This decrease was also observed in the group treated with Seprafilm, which showed no difference from the rtPA + CMC group. We conclude that, in this reproducible adhesion model, only the combination of rtPA + CMC and Seprafilm significantly reduced both the strength and the extent of intraperitoneal adhesions.
Brain levels and metabolism of the dopaminergic agonist 2-amino-6,7-dihydroxytetrahydronaphthalene after administration of various prodrugs (1982)

The synthesis of four prodrug diesters (diacetyl, diisobutyryl, dipivaloyl, and dibenzoyl) of the potent dopaminergic agonist 2-amino-6,7-dihydroxytetrahydronaphthalene (6,7-ADTN) is described. The effects of prodrug structure on the levels of 6,7-ADTN in the rat corpus striatum and cerebellum, as well as the levels of the metabolite, 6-hydroxy-7-methoxy-2-aminotetralin, in the corpus striatum, have been determined after intraperitoneal administration. In addition, the striatal levels of 6,7-ADTN after administration of the dibenzoyl analogue via intraperitoneal, subcutaneous, and oral routes have been measured. These prodrugs produce a significant improvement in the penetration and accumulation of 6,7-ADTN in the brain.
CBF and oxygen metabolism in hemodialysis patients: effects of anemia correction with recombinant human EPO (1992)

To investigate the effects of anemia correction with recombinant human erythropoietin (rhEPO) on both cerebral blood flow and oxygen metabolism in hemodialysis (HD) patients, the regional cerebral blood flow (rCBF), oxygen extraction (rOEF), and metabolic rate for oxygen (rCMRO2) were measured by positron emission tomography in five HD patients, and the data were compared with eight nondemented controls who had neither anemia nor uremia. In the HD patients, 1,500 U of rhEPO were administered intravenously three times a week to achieve a 10% increase in hematocrit (Hct) as an absolute value. Before rhEPO administration, the hemispheric rCMRO2 in HD patients was 1.48 +/- 0.09 (SE) ml.min-1.100 ml-1, which was significantly lower than that of 2.18 +/- 0.10 in the controls (P less than 0.01). In contrast, both rCBF and rOEF were significantly greater in the HD patients than the control, being 40 +/- 3 vs. 32 +/- 3 ml.min-1.100 ml-1 for rCBF (P less than 0.02) and 49 +/- 1 vs. 38 +/- 1% for rOEF (P less than 0.001). After treatment with rhEPO, Hct rose significantly from 21 +/- 1 to 31 +/- 1% in HD patients (P less than 0.001). There were significant reductions in both the hemispheric rCBF to 32 +/- 1 ml.min-1.100 ml-1 (P less than 0.01) and rOEF to 42 +/- 1% (P less than 0.01). However, the hemispheric rCMRO2 remained low at 1.58 +/- 0.06 ml.min-1.100 ml-1 even after rhEPO treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
PCR MIMICS: competitive DNA fragments for use as internal standards in quantitative PCR. (1993)

A rapid and reliable method is described for preparing competitive DNA fragments for quantitative PCR. Synthetic DNAs complementary to previously established PCR primers are ligated together with the primers to both ends of a generic DNA fragment whose length differs from the natural target gene PCR product. After a short ligation step, the properly constructed ligation products (i.e., those that have the correct primer templates on opposite sides of the generic DNA fragment) are preferentially amplified by PCR. The generation of competitive PCR fragments, MIMICS, can be completed in a single day. To perform quantitative PCR, known quantities of PCR MIMICS are spiked into PCR amplification reactions containing the experimental cDNA samples. A visual or radioactive comparison of the PCR products can then be used to determine the initial quantity of target gene. We show that competitive PCR MIMICS can be used to accurately measure small changes in mRNA levels.
Crowd modelling in collaborative virtual environments (1998)

Article Crowd modelling in collaborative virtual environments Share on Authors: Soraia R. Musse Computer Graphics Lab., Swiss Federal Institute of Technology, EPFL, DI-LIG, CH 1015 Lausanne, Switzerland Computer Graphics Lab., Swiss Federal Institute of Technology, EPFL, DI-LIG, CH 1015 Lausanne, SwitzerlandView Profile , Christian Babski Computer Graphics Lab., Swiss Federal Institute of Technology, EPFL, DI-LIG, CH 1015 Lausanne, Switzerland Computer Graphics Lab., Swiss Federal Institute of Technology, EPFL, DI-LIG, CH 1015 Lausanne, SwitzerlandView Profile , Tolga Capin Computer Graphics Lab., Swiss Federal Institute of Technology, EPFL, DI-LIG, CH 1015 Lausanne, Switzerland Computer Graphics Lab., Swiss Federal Institute of Technology, EPFL, DI-LIG, CH 1015 Lausanne, SwitzerlandView Profile , Daniel Thalmann Computer Graphics Lab., Swiss Federal Institute of Technology, EPFL, DI-LIG, CH 1015 Lausanne, Switzerland Computer Graphics Lab., Swiss Federal Institute of Technology, EPFL, DI-LIG, CH 1015 Lausanne, SwitzerlandView Profile Authors Info & Claims VRST '98: Proceedings of the ACM symposium on Virtual reality software and technologyNovember 1998 Pages 115–123https://doi.org/10.1145/293701.293716Online:02 November 1998Publication History 42citation1,331DownloadsMetricsTotal Citations42Total Downloads1,331Last 12 Months22Last 6 weeks4 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Possible Roles of Methyl Glucoside andMyo-inositol in the Opening of Cut Rose Flowers (1999)

Methyl glucoside and myo -inositol are present in all organs of rose ( Rosa hybrida L.). To investigate the possible role of these carbohydrates in the opening of cut roses, flowers with a 10, 20 or 40-cm-long stem and a single flower bud (about 1.5 cm in diameter) were placed in water and flower opening and changes in sugar content in flowers and stems examined for 7 d. The longer the stem of the cut flower, the larger was the flower diameter. In stems, the concentration of carbohydrates, including methyl glucoside and myo -inositol markedly decreased before flower opening. In petals, contents of glucose, methyl glucoside and myo -inositol also decreased before flower opening, but those of fructose, sucrose and xylose did not. When glucose and methyl glucoside were added to the vase water (4%) flower opening was clearly promoted; this was accompanied by an increase in methyl glucoside and fructose concentrations in petals. On the contrary, myo -inositol inhibited flower opening, and this was accompanied by an increase in myo -inositol and xylose concentrations in petals. These results suggest that methyl glucoside and/or its metabolites are transported into the petal cells, thereby lowering the osmotic water potential and promoting flower opening. Myo -inositol is not readily metabolized, and exogenous myo -inositol given at a high concentration may act as an extracellular osmolyte, which inhibits water uptake and flower opening. Copyright 1999 Annals of Botany Company
Protocols for Public Key Cryptosystems (1980)

New Cryptographic protocols which take full advantage of the unique properties of public key cryptosystems are now evolving. Several protocols for public key distribution and for digital signatures are briefly compared with each other and with the conventional alternative.
Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion (2009)

Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood. Systematic examination of the P. placenta genome, transcriptome, and secretome revealed unique extracellular enzyme systems, including an unusual repertoire of extracellular glycoside hydrolases. Genes encoding exocellobiohydrolases and cellulose-binding domains, typical of cellulolytic microbes, are absent in this efficient cellulose-degrading fungus. When P. placenta was grown in medium containing cellulose as sole carbon source, transcripts corresponding to many hemicellulases and to a single putative beta-1-4 endoglucanase were expressed at high levels relative to glucose-grown cultures. These transcript profiles were confirmed by direct identification of peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Also up-regulated during growth on cellulose medium were putative iron reductases, quinone reductase, and structurally divergent oxidases potentially involved in extracellular generation of Fe(II) and H(2)O(2). These observations are consistent with a biodegradative role for Fenton chemistry in which Fe(II) and H(2)O(2) react to form hydroxyl radicals, highly reactive oxidants capable of depolymerizing cellulose. The P. placenta genome resources provide unparalleled opportunities for investigating such unusual mechanisms of cellulose conversion. More broadly, the genome offers insight into the diversification of lignocellulose degrading mechanisms in fungi. Comparisons with the closely related white-rot fungus Phanerochaete chrysosporium support an evolutionary shift from white-rot to brown-rot during which the capacity for efficient depolymerization of lignin was lost.
The Cpf1 CRISPR-Cas protein expands genome-editing tools (2015)

CRISPR-Cas systems have immense biotechnological utility. A recent study reveals the potential of the Cpf1 nuclease to complement and extend the existing CRISPR-Cas9 genome-editing tools.
Respiration of Escherichia coli Can Be Fully Uncoupled via the Nonelectrogenic Terminal Cytochrome bd -II Oxidase (2009)

The respiratory chain of Escherichia coli is usually considered a device to conserve energy via the generation of a proton motive force, which subsequently may drive ATP synthesis by the ATP synthetase. It is known that in this system a fixed amount of ATP per oxygen molecule reduced (P/O ratio) is not synthesized due to alternative NADH dehydrogenases and terminal oxidases with different proton pumping stoichiometries. Here we show that P/O ratios can vary much more than previously thought. First, we show that in wild-type E. coli cytochrome bo, cytochrome bd-I, and cytochrome bd-II are the major terminal oxidases; deletion of all of the genes encoding these enzymes results in a fermentative phenotype in the presence of oxygen. Second, we provide evidence that the electron flux through cytochrome bd-II oxidase is significant but does not contribute to the generation of a proton motive force. The kinetics support the view that this system is as an energy-independent system gives the cell metabolic flexibility by uncoupling catabolism from ATP synthesis under non-steady-state conditions. The nonelectrogenic nature of cytochrome bd-II oxidase implies that the respiratory chain can function in a fully uncoupled mode such that ATP synthesis occurs solely by substrate level phosphorylation. As a consequence, the yield with a carbon and energy source can vary five- to sevenfold depending on the electron flux distribution in the respiratory chain. A full understanding and control of this distribution open new avenues for optimization of biotechnological processes.
Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana. (2006)

Here we report on a simple and reproducible system of Agrobacterium-mediated transient gene expression assay that utilizes infiltration of young Nicotiana benthamiana leaves. Although some of the phenomena described in this paper have been already reported by other researchers, here we have further developed them. The highest level of transient gfp gene expression was detected in the youngest leaves of N. benthamiana infiltrated with A. tumefaciens strains AGL0 and EHA105 precultured in the presence of 450-600 microM acetosyringone. Although the maximum level of transient gfp gene expression was restricted presumably by RNA silencing, it was completely suppressed in the presence of the viral protein HC-Pro. The transient expression system described here can be used to identify new viral suppressors of RNA silencing, for detailed analysis of unidentified genes and for industrial production of proteins in plants as well.