gebhart/patrepeval huggingface dataset:
Array element localization for horizontal arrays via Occam’s inversion (1998)

Accurate locations for the individual elements of an acoustic sensor array are required for the application of advanced array processing methods. This paper develops a general method of localizing horizontal line array (HLA) elements which overcomes bandwidth constraints of low-frequency arrays and uncertainty in the experimental configuration. Array elements are localized for two HLA’s associated with the Spinnaker Array, a three-dimensional sensor array located in the high Arctic. Recordings were made of imploding glass light bulbs deployed at a series of locations surrounding the array site. Implosion instants were not measured; hence, the data consist of relative travel times. In addition, the source locations were measured only approximately in the field, and are treated as unknown parameters. The inverse problem of determining hydrophone and source locations is nonunique and ill-conditioned. To determine the most physically meaningful solution, an iterative linearized inversion is developed which applies the method of regularization to include a priori information about the solution. Available a priori information includes source–location estimates from on-ice measurements, depth estimates for the array end points, and the expectation that each HLA is essentially linear. The inversion is formulated to jointly minimize the parameter-estimate residuals and the three-dimensional curvature of each HLA, subject to fitting the array element localization (AEL) data to a statistically appropriate level. Minimizing HLA curvature produces the simplest array shape that is consistent with the data: any deviations from a straight array are definitely required by the data, and are not artifacts of the inversion algorithm or starting model.
aguA , the Gene Encoding an Extracellular α-Glucuronidase from Aspergillus tubingensis , Is Specifically Induced on Xylose and Not on Glucuronic Acid (1998)

An extracellular alpha-glucuronidase was purified and characterized from a commercial Aspergillus preparation and from culture filtrate of Aspergillus tubingensis. The enzyme has a molecular mass of 107 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 112 kDa as determined by mass spectrometry, has a determined pI just below 5.2, and is stable at pH 6.0 for prolonged times. The pH optimum for the enzyme is between 4.5 and 6.0, and the temperature optimum is 70 degrees C. The alpha-glucuronidase is active mainly on small substituted xylo-oligomers but is also able to release a small amount of 4-O-methylglucuronic acid from birchwood xylan. The enzyme acts synergistically with endoxylanases and beta-xylosidase in the hydrolysis of xylan. The enzyme is N glycosylated and contains 14 putative N-glycosylation sites. The gene encoding this alpha-glucuronidase (aguA) was cloned from A. tubingensis. It consists of an open reading frame of 2,523 bp and contains no introns. The gene codes for a protein of 841 amino acids, containing a eukaryotic signal sequence of 20 amino acids. The mature protein has a predicted molecular mass of 91,790 Da and a calculated pI of 5.13. Multiple copies of the gene were introduced in A. tubingensis, and expression was studied in a highly overproducing transformant. The aguA gene was expressed on xylose, xylobiose, and xylan, similarly to genes encoding endoxylanases, suggesting a coordinate regulation of expression of xylanases and alpha-glucuronidase. Glucuronic acid did not induce the expression of aguA and also did not modulate the expression on xylose. Addition of glucose prevented expression of aguA on xylan but only reduced the expression on xylose.
The Klotho gene family and the endocrine fibroblast growth factors (2008)

Recent mouse genetic studies have broadened our understanding of molecular pathways involved in mineral and bile acid homeostasis regulated by FGF23 and FGF19, respectively. The FGF19 subfamily of ligands requires the Klotho gene family of transmembrane proteins for their tissue-specific bioactivity. Further investigations on endocrine axes mediated by the Klotho family and FGF19 subfamily members are expected to provide new insights into the molecular mechanisms by which the endocrine fibroblast growth factors regulate bile acid, energy, and phosphate/vitamin D metabolism.
Alleviation of Systemic Manifestations of Castleman's Disease by Monoclonal Anti-Interleukin-6 Antibody (1994)

Castleman's disease (angiofollicular lymphoid hyperplasia) is a heterogeneous group of lymphoproliferative disorders of uncertain cause1. Two pathologic types, hyaline vascular and plasma-cell disease, have been recognized. The plasma-cell variant of Castleman's disease may be localized or multicentric. Multicentric disease is a systemic lymphoproliferative disorder characterized by lymphadenopathy, hepatosplenomegaly, and constitutional symptoms. Anemia, hypoalbuminemia, and hypergammaglobulinemia are also common. Interleukin-6, a cytokine with pleiotropic effects on the immune system, hematopoiesis, and acute-phase reactions, is a putative growth factor in multiple myeloma and may also be central to the pathophysiology of Castleman's disease2–7. Administration of a murine anti-interleukin-6 monoclonal . . .
Undifferentiated U937 cells transfected with chemoattractant receptors: a model system to investigate chemotactic mechanisms and receptor structure/function relationships (1997)

Transfection of either the C5a receptor or the formyl peptide receptor into undifferentiated U937 cells generated continuously growing cell lines that stably expressed these receptors. The transfected cells displayed significant numbers of cell surface receptors that had ligand binding properties similar to fully differentiated U937 cells. Undifferentiated transfected U937 cells were capable of a ligand-specific calcium flux and showed migratory responses that were qualitatively and quantitatively similar to differentiated cells and were specific for each chemoattractant. Moreover, the chemotactic response could be desensitized by preincubating the cells in a high concentration of ligand and could be blocked by pertussis toxin. These results demonstrate that undifferentiated U937 cells possess the subcellular signaling apparatus and machinery necessary to generate a motile response and that the only missing component for chemotaxis is expression of a chemoattractant receptor. In addition, the results demonstrate that undifferentiated U937 cells transfected with chemoattractant receptors provide a defined model system to study receptor structure/function relationships and may be used to investigate receptor-mediated chemotactic responses in a relevant human myeloid cell.
Risk of disability and mortality due to overweight in a Finnish population. (1990)

Though modest overweight has little impact on mortality it predicts severe functional impairment. A considerable proportion of work disability pensions could probably be prevented by efficient weight control.
Advances in Contrast Enhancement for Optical Coherence Tomography (2006)

Contrast in optical coherence tomography (OCT) images is often limited, particularly when pathological tissue is morphologically or optically similar to normal tissue. In recent years, there has been increasing interest in developing methods for enhancing OCT contrast. In general, contrast can be enhanced by the administration of passive or targeted exogenous contrast agents, or by exploiting linear and nonlinear techniques for sampling the endogenous molecular composition of tissue. Many exogenous agents, in addition to being targeted to specific cells and tissues, can also serve as multifunctional agents, delivering or facilitating therapy as well as providing enhanced contrast for imaging and localization. This paper and presentation will discuss novel OCT contrast enhancing methods designed to selectively identify tissues of interest.
An ancestry informative marker set for determining continental origin: validation and extension using human genome diversity panels (2009)

These data provide additional support for using the 93 AIM set to efficiently identify continental subject groups for genetic studies, to identify study population outliers, and to control for admixture in association studies.
Prevention of graft-versus-host disease by selective depletion of CD6-positive T lymphocytes from donor bone marrow. (1992)

We conclude that selective in vitro T-cell depletion with an anti-T12 monoclonal antibody effectively reduces the incidence of both acute and chronic GVHD after allogeneic BMT without compromising engraftment. Moreover, depletion of CD6-positive cells from donor marrow obviates the need to administer immune suppressive medications to the majority of patients. This approach reduces the morbidity and mortality of allogeneic BMT and permits the BMT of older patients.
Targeted Disruption of LIGHT Causes Defects in Costimulatory T Cell Activation and Reveals Cooperation with Lymphotoxin β in Mesenteric Lymph Node Genesis (2002)

The recently described tumor necrosis factor (TNF) family member LIGHT (herpes virus entry mediator [HVEM]-L/TNFSF14), a ligand for the lymphotoxin (LT)beta receptor, HVEM, and DcR3, was inactivated in the mouse. In contrast to mice deficient in any other member of the LT core family, LIGHT(-/-) mice develop intact lymphoid organs. Interestingly, a lower percentage of LIGHT(-/-)LTbeta(-/-) animals contain mesenteric lymph nodes as compared with LTbeta(-/-) mice, whereas the splenic microarchitecture of LIGHT(-/-)LTbeta(-/-) and LTbeta(-/-) mice shows a comparable state of disruption. This suggests the existance of an additional undiscovered ligand for the LTbeta receptor (LTbetaR) or a weak LTalpha(3)-LTbetaR interaction in vivo involved in the formation of secondary lymphoid organs. LIGHT acts synergistically with CD28 in skin allograft rejection in vivo. The underlying mechanism was identified in in vitro allogeneic MLR studies, showing a reduced cytotoxic T lymphocyte activity and cytokine production. Detailed analyses revealed that proliferative responses specifically of CD8+ T cells are impaired and interleukin 2 secretion of CD4+ T cells is defective in the absence of LIGHT. Furthermore, a reduced 3[H]-thymidine incorporation after T cell receptor stimulation was observed. This for the first time provides in vivo evidence for a cooperative role for LIGHT and LTbeta in lymphoid organogenesis and indicates important costimulatory functions for LIGHT in T cell activation.
Clostridium algidixylanolyticum sp. nov., a psychrotolerant, xylan-degrading, spore-forming bacterium. (2000)

A psychrotolerant Clostridium species was isolated from vacuum-packed, temperature-abused raw lamb. Colonies of this micro-organism on sheep-blood agar were circular with an entire margin, grey-white, translucent and beta-haemolytic. Cells were single, tapered, motile rods. Elliptical subterminal spores were produced in the late stationary growth phase. Spores did not cause swelling of the maternal cells. The micro-organism was obligately anaerobic. In peptone yeast extract glucose starch (PYGS) broth at pH 7.0, the micro-organism grew optimally between 25.5 and 30.0 degrees C. The temperature range for growth was 2.5-32.2 degrees C. At 26 degrees C, the micro-organism grew optimally at pH 6.8 to 7.0. The pH range for anaerobic growth was 4.7-9.1. The micro-organism was saccharoclastic, hydrolysed starch and degraded xylan. The fermentation products formed in PYGS broth were acetate, formate, lactate, ethanol, butyrate, butanol, hydrogen and carbon dioxide. The G + C content of the DNA was 38.4 mol%. Phylogenetic analyses indicated that the strain belongs to cluster XIVa of the genus Clostridium (sensu Collins et al. 1994). The new strain differed from phylogenetically related clostridia in terms of cellular fatty acid composition, soluble protein profiles and phenotypic properties. On the basis of phenotypic and genotypic characterization data, the strain was assigned to a new species, namely Clostridium algidixylanolyticum. The type strain is strain SPL73T (= DSM 12273T).
Grooming the Fleet (2010)

This article presents an overview of a robotic system, named Hull BUG, designed to remove deposits from the undersides of ships. The robot is the product of SeaRobotics, a company in Palm Beach Gardens, FL, USA, that develops unmanned vessels for jobs including deep-water research and coastal surveillance. The current developmental model of the Hull BUG uses a negative pressure vortex regenerative fluid movement assembly to attach itself to the hull. The robot is designed to work without using cleaning chemicals. It measures about 2-feet wide, 2-feet long, and 1-foot high. Weighing 30 pounds, it is designed to be neutrally buoyant in seawater, so it needs less propulsion to negotiate a ship’s hull underwater. The Hull BUG requires no human operator. Onboard sensors provide obstacle avoidance, path planning, and navigation capabilities. These capabilities include a biofilm detector that can distinguish between fouled and groomed surfaces. The Hull BUG has already been tested on many pieces of steel having different types of structures and shapes to model sections of ship hulls.
Endoscopic camera control by head movements for thoracic surgery (2010)

In current video-assisted thoracic surgery, the endoscopic camera is operated by an assistant of the surgeon, which has several disadvantages. This paper describes a system which enables the surgeon to control the endoscopic camera without the help of an assistant. The system is controlled using head movements, so the surgeon can use his/her hands to operate the instruments. The system is based on a flexible endoscope, which leaves more space for the surgeon to operate his/her instruments compared to a rigid endoscope. The endoscopic image is shown either on a monitor or by means of a head-mounted display. Several trial sessions were performed with an anatomical model. Results indicate that the developed concept may provide a solution to some of the problems currently encountered in video-assisted thoracic surgery. The use of a head-mounted display turned out to be a valuable addition since it ensures the image is always in front of the surgeon's eyes.
Adhesion and Activation of Human Platelets Induced by Convulxin Involve Glycoprotein VI and Integrin α21 (1997)

We analyzed the interaction of convulxin (Cvx), a 72-kDa protein isolated from the venom of Crotalus durissus terrificus, with human platelets. Cvx is a potent platelet agonist that induces an increase in the intracellular Ca2+ concentration ([Ca2+]i), granule exocytosis and aggregation. 125I-Labeled Cvx binds specifically and rapidly to platelets at binding sites of high and moderate affinity. Platelets adhere to immobilized Cvx in a time-dependent but cation-independent manner. Platelet exocytosis and aggregation induced by Cvx were inhibited by an anti-integrin alpha2beta1 monoclonal antibody (6F1) and by the Fab fragments of a polyclonal anti-glycoprotein VI (GPVI) antibody. Both the adhesion of platelets to Cvx and the Cvx-induced increase in [Ca2+]i were inhibited by anti-GPVI Fab fragments but not by 6F1. Ligand blotting assay showed that 125I-Cvx binds to a 57-kDa platelet protein with an electrophoretic mobility identical to that of GPVI. In addition, we observed the following: (i) 125I-Cvx binds to GPVI immunoprecipitated by the anti-GPVI antibody from a platelet lysate, and (ii) Cvx inhibits the binding of anti-GPVI IgG to GPVI. Taken together, these results demonstrate that GPVI behaves as a Cvx receptor and that the alpha2beta1 integrin appears to be involved in the later stages of Cvx-induced platelet activation, i.e. exocytosis and aggregation.
Nitric Oxide: Surface Reactions and Removal from Auto Exhaust (1975)

Abstract In the last 10 years an extensive research and development effort has been made to implement the catalytic removal of noxious constituents from automotive exhaust. This effort is finally coming to fruition as this paper is going into print. Vehicles sold in the United States in 1975 will be, in a significant proportion, equipped with catalytic devices. The incorporation of a miniature chemical conversion plant into millions of moving objects operating under widely varying conditions, while being required to assure reliable performance for extended usage periods, has called for the combined skills of scientists, engineers, and designers in several industries. Even so, at present only the catalytic oxidation of carbon monoxide and hydrocarbons has reached the implementation stage. The catalytic removal of nitrogen oxides has turned out to be a still more difficult task. This review will summarize very briefly the present state-of-art of the catalytic devices for the treatment of NO, emissions, but its larger part will be concerned with the scientific background underlying the operation of the devices themselves.
Adenosine 5'-triphosphate-sensitive potassium channel-mediated blood-brain tumor barrier permeability increase in a rat brain tumor model. (2003)

Brain tumor microvessels/capillaries limit drug delivery to tumors by forming a blood-brain tumor barrier (BTB). The BTB overexpresses ATP-sensitive potassium (K(ATP)) channels that are barely detectable in normal brain capillaries, and which were targeted for BTB permeability modulation. In a rat brain tumor model, we infused minoxidil sulfate (MS), a selective K(ATP) channel activator, to obtain sustained, enhanced, and selective drug delivery, including various sized molecules, across the BTB to brain tumors. Glibenclamide, a selective K(ATP) channel inhibitor, significantly attenuated the MS-induced BTB permeability increase. Immunocytochemistry and glibenclamide binding studies showed increased K(ATP) channel density distribution on tumor cells and tumor capillary endothelium, which was confirmed by K(ATP) channel potentiometric assay in tumor cells and brain endothelial cells cocultured with brain tumor cells. MS infusion in rats with brain tumors significantly increased transport vesicle density in tumor capillary endothelial and tumor cells. MS facilitated increased delivery of macromolecules, including Her-2 antibody, adenoviral-green fluorescent protein, and carboplatin, to brain tumors, with carboplatin significantly increasing survival in brain tumor-bearing rats. K(ATP) channel-mediated BTB permeability increase was also demonstrated in a human, brain tumor xenograft model. We conclude that K(ATP) channels are a potential target for biochemical modulation of BTB permeability to increase antineoplastic drug delivery selectively to brain tumors.
Molecular basis of hepatitis B virus serotype variations within the four major subtypes (1992)

Amino acid residues 101 to 180 of hepatitis B surface antigen (HBsAg) were predicted by sequencing the corresponding part of the S gene of hepatitis B virus (HBV) DNA in 46 HBsAg-positive sera, which had been subtyped by immunodiffusion with respect to d/y, w/r, w1 to w4 and q. The sequences of the nine different HBV serotypes defined by these specificities were found to be homogeneous proving that they represent consistent variations of HBV at the genomic level. Residue 127 was found to be important as were Pro, Thr and Leu for w1/w2, w3 and w4, respectively. Five residues were found to differ between ayw1 and ayw2. These were at positions 134 (Phe instead of Tyr), 143 (Thr instead of Ser), 159 (Ala instead of Gly), 161 (Tyr instead of Phe) and 168 (Val instead of Ala). However, all these residues were shared by ayw1 and adw2, implying that Arg122 was also important for w1 expression. All genomes expressing r, apart from one ayr strain, had an Ile126, which might explain the pseudo-allelism of w1 to w4 in relation to r, since this substitution might influence the w epitope. There were two regions where adw4q- and adrq- differed from all the q+ subtypes. These were located at residues 158 and 159, and at residues 177 and 178, where both the q- subtypes had amino acid substitutions in adjacent positions. The mapping of the epitopes defining these antigenic specificities will help to link information on the world-wide distribution of HBsAg subtypes to future molecular epidemiology with regard to HBV.
A new drain-current injection technique for the measurement of off-state breakdown voltage in FETs (1993)

A simple three-terminal technique for measuring the off-state breakdown voltage of FETs is presented. With the source grounded, current is injected into the drain of the on-state device. The gate is then ramped down to shut the device off. In this process, the drain-source voltage rises to a peak and then drops. This peak represents an unambiguous definition of three-terminal breakdown voltage. In the same scan, a measurement of the two-terminal gate-drain breakdown voltage is also obtained. The method offers potential for use in a manufacturing environment, as it is fully automatable. It also enables easy measurement of breakdown voltage in unstable and fragile devices.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Regulation of Human Hair Growth by Steroid Hormones. II. Androstenedione Metabolism in Isolated Hairs (1974)

Androstenedione-1,2-3H metabolism has been measured under standardized conditions in growing and resting hair roots from varying anatomical sites from 5 women and 12 men, 6 of whom had male pattern baldness. 5α-Reduced and 17β-hydroxysteroids were the major metabolites of androstenedione. Hairs from all body regions have the capacity to transform androstenedione into testosterone, but the rate of the reduction of 17-ketosteroids is less than the rate of oxidation so that the 17-keto rather than 17β-hydroxysteroids tend to accumulate in the cell. Since the 5α-reduction is irreversible and conditions favor the formation of 17-ketosteroids, androstanedione is the principal intracellular androgen in the hair root.
Multicenter Phase II Study of the Oral MEK Inhibitor, CI-1040, in Patients With Advanced Non-Small-Cell Lung, Breast, Colon, and Pancreatic Cancer (2004)

CI-1040 was generally well tolerated but demonstrated insufficient antitumor activity to warrant further development in the four tumors tested. PD 0325901, a second generation MEK inhibitor, has recently entered clinical development and, with significantly improved pharmacologic and pharmaceutical properties compared with CI-1040, it may better test the therapeutic potential of MEK inhibition in cancer.
Hepatic progenitor cells in human fetal liver express the oval cell marker Thy-1 (2006)

Hepatic progenitor cells play a major role in regenerating diseased liver. In rodents, progenitors forming hepatocytes or cholangiocytes are identified by the stem cell marker Thy-1. The aim of this study was to ascertain whether progenitor cells expressing Thy-1 could be identified in human fetal liver. Midtrimester human fetal liver was immunostained for Thy-1, cytokeratins 18 and 19, vimentin, CD34, CD45, and fibrinogen. Thy-1+ and Thy-1+CD34+ populations were purified using fluorescence-activated cell sorting (FACS). Immunofluorescence and mRNA expression were used to examine the bipotential nature of purified stem cells. We found that Thy-1+ cells were concentrated in portal tracts but were also scattered in parenchyma. In FACS-prepared cells, 0.18-3.08% (median 0.65%, n = 14) of cells were Thy-1+. Immunophenotyping revealed that some Thy-1+ cells coexpressed cytokeratins 18 and 19, others, fibrinogen and cytokeratin 19. RT-PCR demonstrated that Thy-1+ cells expressed mRNA for Thy-1, cytokeratin 18, and cytokeratin 19, and Thy-1+CD34+ cells expressed mRNA for alpha-fetoprotein, transferrin, and hepatocyte nuclear factor-4alpha. Thy-1+ cells were identified in fetal liver. These cells expressed several lineage markers, including coexpression of biliary and hepatocellular proteins and mRNA. These data suggest that Thy-1 is a marker of liver stem cells in human fetal liver.