gebhart/patrepeval huggingface dataset:
Successful Wide Hybridization Between the Soybean and a Wild Perennial Relative, G. tomentella Hayata1 (1982)

An important source of genetic diversity that has been employed effectively in a variety of breeding programs is that provided by wild relatives of crop plants. In this paper, we report that hybrids between the soybean [ Glycine max (L.) Merr.] and a wild perennial relative, G. tomentella Hayata, were obtained for the first time through in vitro ovule culture. The F 1 plants were vegetatively vigorous and resembled the wild male parent morphologically. The chromosome numbers were consistent with a hybrid origin. The plants flowered profusely but were sterile. The data suggest that the use of wild perennial members of Glycine might become feasible for broadening the germplasm base of the soybean.
Fluorescent monitoring using microfluidics chip and development of syringe pump for automation of enucleation to automate cloning (2009)

We have developed a novel technique for fluorescent monitoring of a bovine oocyte nucleus for an automatic cloning device. Animal cells that have been chemically softened by cytochalasin and stained with Hoechst dye are aspirated into a thin microchannel of a microfluidic chip and stretched thin, allowing the nucleus of the expanded oocyte to be monitored. Half the volume of the oocyte is aspirated into the thin microchannel and a high-velocity fluid flow is generated in the wide microchannel to bisect the oocyte. Then, the half-oocytes are monitored to determine which contains the nucleus. To control flow velocity with high accuracy and rapid response, we also developed a syringe pump that is small, has no backlash, and has highly-accurate volume control and a good response for automatic cutting. In this report, we describe the monitoring method and construction of the syringe pump.
In vivo patterns of expression of urokinase and its inhibitor PAI-1 suggest a concerted role in regulating physiological angiogenesis. (1992)

To evaluate the role of plasminogen activators (PAs) in physiological angiogenesis, we have investigated the in vivo patterns of expression of urokinase-type PA (uPA) and PA-inhibitor type 1 (PAI-1) during neovascularization of ovarian follicles, the corpus luteum, and the maternal decidua. Using in situ hybridization, we detected uPA mRNA in the ovary along the route of capillary extension, originating at the existing ovarian vasculature, extending toward growing follicles, and terminating at the newly formed capillary sheaths surrounding each growing follicle. Following ovulation, uPA mRNA was expressed in capillary sprouts within the developing corpus luteum. During the process of decidual neovascularization, uPA expression was detected in endothelial cell cords traversing the maternal decidua in the direction of the newly implanted embryo. uPA mRNA was not detected in endothelial cells upon completion of neovascularization, suggesting that uPA expression is a part of the angiogenic response. During in vitro "angiogenesis" of cultured aortic explants, uPA was expressed in capillary sprouts but not in underlying endothelial cell sheets, suggesting that the expression of uPA depends on the histological context of the endothelial cell. Interestingly, during corpus luteum development and decidual neovascularization, and in aortic explants, PAI-1 expression was preferentially activated in cells in the vicinity of uPA-expressing capillary-like structures. These findings suggest a functional interplay between uPA- and PAI-1-expressing cells and support the idea that natural PA inhibitors function during angiogenesis to protect neovascularized tissues from excessive proteolysis.
Lock reservation (2002)

Because of the built-in support for multi-threaded programming, Java programs perform many lock operations. Although the overhead has been significantly reduced in the recent virtual machines, One or more atomic operations are required for acquiring and releasing an object's lock even in the fastest cases.This paper presents a novel algorithm called lock reservation. It exploits thread locality of Java locks, which claims that the locking sequence of a Java lock contains a very long repetition of a specific thread. The algorithm allows locks to be reserved for threads. When a thread attempts to acquire a lock, it can do without any atomic operation if the lock is reserved for the thread. Otherwise, it cancels the reservation and falls back to a conventional locking algorithm.We have evaluated an implementation of lock reservation in IBM's production virtual machine and compiler. The results show that it achieved performance improvements up to 53% in real Java programs.
Primary Repair Of Flexor Tendons In The Hand Without Immobilisation – Preliminary Report (1978)

A new approach to the problem of flexor tendon repair within the fibro-osseous canal is presented. Using a technique of bevelling the tendon ends and suturing with a fine suture material, under magnificaiton, a sufficiently strong junction is obtained, which enables immediate active mobilisation without strangulation of the blood supply. The junction can resist gap formation up to tensions of 4 Kg. It is postulated that under these conditions tendon nutrition is minimally interfered with, adhesions do not form, and the tendon heals by its own intrinsic healing ability.
Redirected touching: Warping space to remap passive haptics (2010)

There is an increasing interest in deployable virtual military training systems. Haptic feedback for these training systems can enable users to interact more naturally with the training environment, but is difficult to deploy. Passive haptic feedback is very compelling, but it is also inflexible. Changes made to virtual objects can require time-consuming changes to their physical passive-haptic counterparts. This poster explores the possibility of mapping many differently shaped virtual objects onto one physical object by warping virtual space and exploiting the dominance of the visual system. A first implementation that maps different virtual objects onto dynamically captured physical geometry is presented, and potential applications to deployable military trainers are discussed.
Clonal analysis in the intact mouse embryo by intragenic homologous recombination. (1993)

A method of cell labelling based on homologous recombination has been developed to analyze the lineage of cells in intact embryos. In this method a LacZ gene bearing an inactivating duplication in its coding frame (termed LaacZ gene) is inserted into the genome of mice. Subsequently, in transgenic embryos, homologous recombination within the duplication recreates an open reading frame for beta-galactosidase. The descendants of the modified cell are identified histochemically. This recombination occurs at random and with low frequency. It can occur in cells in which the gene is not transcribed. It can also be intragenic (in adjacent sequences) as single transgene copies are found to recombine. To analyze the lineage of cells in the myotome the expression of the LaacZ gene is driven by a promoter which confers expression specifically to cells of this compartment. The analysis in 40-somite embryos (E11.5) is reported. We show that individual beta-gal+ myotomal clones may contribute to somites from both sides of the animal, in which case the right and the left somites are labelled consecutively and at the same antero-posterior level. It reflects predictable clonal origin of myotomal cells at the primitive streak stage or before and demonstrates three morphogenetic movements in the mesoderm: bilateral, antero-posterior and mediolateral. The results also show that the left-right assignation of myotomal cells in mouse is late, probably occurring during cell migrations at gastrulation. It applies also to presumptive myotomal cells of limb musculature. These results contrast with early left-right assignation of most tissues in Xenopus and Zebrafish. Some clones contribute to distant somites, suggesting persistence of presumptive myotomal cells in the presumptive paraxial mesoderm and clonal organisation of the myotome.
Nonviral Vectors for Gene Delivery (2008)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTNonviral Vectors for Gene DeliveryMeredith A. Mintzer and Eric E. Simanek*View Author Information Department of Chemistry, Texas A&M University, College Station, Texas 77843* To whom correspondence should be addressed. Tel.: (979) 845-4242. Fax: (979) 845-9452. E-mail: [email protected]Cite this: Chem. Rev. 2009, 109, 2, 259–302Publication Date (Web):December 3, 2008Publication History Received10 June 2008Published online3 December 2008Published inissue 11 February 2009https://pubs.acs.org/doi/10.1021/cr800409ehttps://doi.org/10.1021/cr800409ereview-articleACS PublicationsCopyright © 2008 American Chemical SocietyRequest reuse permissionsArticle Views25002Altmetric-Citations2038LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Chemical structure,Gene delivery,Genetics,Polyethylenimine,Toxicity Get e-Alerts
Controlling Flows in Microchannels with Patterned Surface Charge and Topography (2003)

This Account reviews two procedures for controlling the flow of fluids in microchannels. The first procedure involves patterning the density of charge on the inner surfaces of a channel. These patterns generate recirculating electroosmotic flows in the presence of a steady electric field. The second procedure involves patterning topography on an inner surface of a channel. These patterns generate recirculation in the cross-section of steady, pressure-driven flows. This Account summarizes applications of these flow to mixing and to controlling dispersion (band broadening).
Building visual language parsers (1991)

Article Building visual language parsers Share on Authors: Richard Helm I.B.M. Thomas J. Watson Research Center, P.O. Box 704, Yorktown Heights, NY I.B.M. Thomas J. Watson Research Center, P.O. Box 704, Yorktown Heights, NYView Profile , Kim Marruitt I.B.M. Thomas J. Watson Research Center, P.O. Box 704, Yorktown Heights, NY I.B.M. Thomas J. Watson Research Center, P.O. Box 704, Yorktown Heights, NYView Profile , Martin Odersky I.B.M. Thomas J. Watson Research Center, P.O. Box 704, Yorktown Heights, NY I.B.M. Thomas J. Watson Research Center, P.O. Box 704, Yorktown Heights, NYView Profile Authors Info & Claims CHI '91: Proceedings of the SIGCHI Conference on Human Factors in Computing SystemsApril 1991 Pages 105–112https://doi.org/10.1145/108844.108860Online:01 March 1991Publication History 44citation713DownloadsMetricsTotal Citations44Total Downloads713Last 12 Months33Last 6 weeks3 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Hyperactivity and hyperconnectivity of the default network in schizophrenia and in first-degree relatives of persons with schizophrenia (2009)

We examined the status of the neural network mediating the default mode of brain function, which typically exhibits greater activation during rest than during task, in patients in the early phase of schizophrenia and in young first-degree relatives of persons with schizophrenia. During functional MRI, patients, relatives, and controls alternated between rest and performance of working memory (WM) tasks. As expected, controls exhibited task-related suppression of activation in the default network, including medial prefrontal cortex (MPFC) and posterior cingulate cortex/precuneus. Patients and relatives exhibited significantly reduced task-related suppression in MPFC, and these reductions remained after controlling for performance. Increased task-related MPFC suppression correlated with better WM performance in patients and relatives and with less psychopathology in all 3 groups. For WM task performance, patients and relatives had greater activation in right dorsolateral prefrontal cortex (DLPFC) than controls. During rest and task, patients and relatives exhibited abnormally high functional connectivity within the default network. The magnitudes of default network connectivity during rest and task correlated with psychopathology in the patients. Further, during both rest and task, patients exhibited reduced anticorrelations between MPFC and DLPFC, a region that was hyperactivated by patients and relatives during WM performance. Among patients, the magnitude of MPFC task suppression negatively correlated with default connectivity, suggesting an association between the hyperactivation and hyperconnectivity in schizophrenia. Hyperactivation (reduced task-related suppression) of default regions and hyperconnectivity of the default network may contribute to disturbances of thought in schizophrenia and risk for the illness.
A Growth Hormone Agonist Produced by Targeted Mutagenesis at Binding Site 1. (1995)

Growth hormone (GH) is believed to signal by dimerizing its receptor through two binding sites on the hormone. Previous attempts to increase the biopotency of GH by increasing its site 1 affinity have been unsuccessful, which has led to a bias toward engineering site 2 interactions in the quest for creation of super agonists. Here we report that increasing site 1 affinity can markedly increase proliferative bioactivity in FDC-P1 cells expressing full-length GHR. In contrast, we find three site 1 mutants with affinities for site one similar to or greater than wild type GH, which have markedly decreased bioactivity. Through crystal structure analysis of the receptor interactive regions of these GH analogues, we are able to suggest why previous mutagenesis on human GH failed to improve biopotency, and thus provide a new avenue for GH and cytokine agonist design.
Dissociation of tissue uptake of cholesterol ester from that of apoprotein A-I of rat plasma high density lipoprotein: selective delivery of cholesterol ester to liver, adrenal, and gonad. (1983)

The metabolic fate of homologous high density lipoprotein (HDL) was studied in the rat, tracing the apoprotein A-I (apo A-I) and cholesterol ester moieties simultaneously. The apo A-I was labeled with covalently linked 125I-labeled tyramine cellobiose, which accumulates in the cells degrading the apoprotein; [3H]cholesterol ethers, which cannot be hydrolyzed or mobilized after uptake, were incorporated into the lipid core of reconstituted HDL to reflect the fate of the cholesterol esters. Several lines of evidence, including direct comparison with biologically labeled HDL, are presented to support the validity of this approach. The liver was the major organ of cholesterol ether uptake, accounting for 65% of the total; the adrenal gland and ovary were the most active organs per gram (wet) of weight. Uptake of cholesterol ether was 7-fold greater than that of apo A-I in adrenal, 4-fold greater in the ovary, and greater than 2-fold greater in the liver. The remaining tissues took up apo A-I and cholesterol ethers at more nearly equal rates. Transfer of HDL-associated cholesterol ethers and 125I-labeled apo A-I to other lipoprotein fractions was not observed; thus, the results reflect direct uptake from HDL itself. Whereas uptake of low density lipoprotein appears to involve endocytosis of intact particles, uptake of HDL in at least some rat tissues involves additional, more complex, transfer mechanisms.
TREM-1 ligand expression on platelets enhances neutrophil activation (2007)

The triggering receptor expressed on myeloid cells 1 (TREM-1) plays an important role in the innate immune response related to severe infections and sepsis. Modulation of TREM-1-associated activation improves the outcome in rodent models for pneumonia and sepsis. However, the identity and occurrence of the natural TREM-1 ligands are so far unknown, impairing the further understanding of the biology of this receptor. Here, we report the presence of a ligand for TREM-1 on human platelets. Using a recombinant TREM-1 fusion protein, we demonstrate specific binding of TREM-1 to platelets. TREM-1-specific signals are required for the platelet-induced augmentation of polymorphonuclear leukocyte (PMN) effector functions (provoked by LPS). However, TREM-1 interaction with its ligand is not required for platelet/PMN complex formation, which is dependent on integrins and selectins. Taken together, the results indicate that the TREM-1 ligand is expressed by platelets, and the TREM-1/ligand interaction contributes to the amplification of LPS-induced PMN activation. Our results shed new light on our understanding of TREM-1 and its role in the innate inflammatory response in infections and might contribute to the development of future concepts to treat sepsis.
Spatial patterns of spinal cord [14C]-2-deoxyglucose metabolic activity in a rat model of painful peripheral mononeuropathy (1992)

Mao, Jianren∗,a; Price, Donald D.b; Coghill, Robert C.a; Mayer, David J.a; Hayes, Ronald L.c Author Information
Atrial Natriuretic Peptide Inhibits Mitogen-activated Protein Kinase through the Clearance Receptor (1996)

The modulation of the activity of mitogen-activated protein kinase (MAPK) by endogenous growth factors or growth inhibitors provides a potential means of regulating cell proliferation. We determined the effect of the endogenous anti-proliferative peptide, atrial natriuretic peptide (ANP), on the ability of MAPK to phosphorylate myelin basic protein. In astrocytes, MAPK activity was significantly stimulated (up to 3-fold) by three known glial mitogens, endothelin-3, platelet-derived growth factor, or phorbol 12-myristate 13-acetate. ANP inhibited by 55-70% the ability of each of these mitogens to activate MAPK. The effects of ANP were equipotent to those caused by C-ANP 4-23, a peptide that specifically binds to the natriuretic peptide clearance receptor. Additionally, both natriuretic peptides caused a 70-80% inhibition of the sodium vanadate-stimulated MAPK activity, complete inhibition of the okadaic acid-stimulated activity, and inhibition of the mitogen-stimulated phosphorylation of MAPK. To understand the potential mechanism by which the natriuretic peptides act, we found that both ANP and C-ANP inhibited the mitogen-stimulated activity of the immediate upstream kinase in the cascade, MAPK kinase (MEK). C-ANP also strongly inhibited the endothelin-3-, platelet-derived growth factor-, and phorbol 12-myristate 13-acetate-induced stimulation of DNA synthesis in the astrocytes, while both okadaic acid and sodium vanadate significantly reversed these anti-proliferative actions. Our results identify ANP as a peptide hormone that inhibits growth factor-stimulated MAPK. These data suggest that the ability of the natriuretic peptides to inhibit MAPK may be important for their anti-growth actions. This effect likely occurs via the inhibition of upstream kinase(s), including MEK, uniquely resulting from ligand binding to the natriuretic peptide clearance receptor.
Oestrogen Receptors in Macrophages (1990)

The presence of receptors for oestradiol-17 beta and 5 alpha-dihydrotestosterone (5 alpha-DHT) in the human monocytic leukaemia cell line J111 and rat peritoneal macrophages was investigated using whole-cell assays. For both cell types, high-affinity binding species for oestrogen were detected, whereas no indication of specific binding was observed for 5 alpha-DHT. Analysis of the data according to Scatchard showed curved lines, indicating the presence of two different oestrogen-binding species. The dissociation constant (Kd) values for the receptors of the rat peritoneal macrophages were calculated to be 1.4 x 10(-10) M and 3.3 x 10(-9) M, while for the J111 cells, the Kd values were 8.7 x 10(-11) M and 2.5 x 10(-9) M. Sucrose-gradient ultracentrifugation identified one oestrogen-binding species of 7.1S. The receptors had a relatively high affinity for diethylstilboestrol (DES) but did not bind to a monoclonal antibody specific for the classical oestrogen receptor, suggesting that oestrogen receptors in macrophages could be of a different type.
Reagentless Mediated Laccase Electrode for the Detection of Enzyme Modulators (1997)

We have investigated aerobic mediation of electron transfer to a laccase enzyme by the solution redox couples [Os(bpy)(2)Cl(2)](+/0) and [Os(bpy)(2)(MeIm)Cl](2+/+), where bpy is 2,2-bipyridine and MeIm is N-methylimidazole. The factors that influence the homogeneous mediation reaction are investigated and discussed. Investigation of ionic strength, pH, and temperature effects on the kinetics of intermolecular electron transfer elucidates the governing factors in the mediator-enzyme reactions. Coimmobilization of both enzyme and an osmium redox mediator in a hydrogel on glassy carbon electrodes results in a biosensor for the reagentless addressing of enzyme activity, consuming only oxygen present in solution. Thus, these immobilized enzyme biosensors can be utilized for the detection of modulators of laccase activity, such as the inhibitor sodium azide. The enzyme inhibition biosensor can detect levels of azide as low as 2.5 × 10(-6) mol dm(-3) in solution.
Modification of Rab5 with a Photoactivatable Analog of Geranylgeranyl Diphosphate (2001)

A photoprobe analog of geranylgeranyl diphosphate (2-diazo-3,3,3-trifluoropropionyloxy-farnesyl diphosphate or DATFP-FPP) inhibits mevalonate-dependent prenylation of in vitro translated Rab5 in rabbit reticulocyte lysate, suggesting that it competes for lipid binding to the Rab geranylgeranyl transferase. Modification of Rab5 with DATFP-FPP, demonstrated by gel mobility shift and Triton X-114 phase separation experiments, confirms that the enzyme uses the analog as a substrate. The sedimentation of DATFP-modified Rab5 as a larger mass complex on sucrose density gradients indicates that it binds to other factors in rabbit reticulocyte lysate. Most importantly, DATFP-Rab5 cross-links to these soluble factors upon exposure to UV light. Immunoprecipitation with antibodies raised against proteins known to interact with Rab5 reveals that the cross-linked complexes contain Rab escort protein and GDI-1. DATFP-Rab5 also associates with membranes in a guanosine-5'-O-(3-thiotriphosphate)-stimulated manner. However, although prenylated Rab5 can be cross-linked to two unknown membrane-associated factors by the chemical cross-linker disuccinimidyl suberate, these proteins fail to be UV cross-linked to membrane-bound DATFP-Rab5. These results strongly suggest that membrane-associated factors bind Rab5 through protein-protein interactions rather than protein-prenyl interactions. The modification of Rab5 with DATFP-FPP establishes a novel photoaffinity technique for the characterization of prenyl-binding sites.