gebhart/patrepeval huggingface dataset:
Origin of the Difference in Order−Disorder Transition Temperature between Polystyrene-block-poly(2-vinylpyridine) and Polystyrene-block-poly(4-vinylpyridine) Copolymers (2007)

Three polystyrene-block-poly(2-vinylpyridine) (S2VP diblock) and three polystyrene-block-poly(4-vinylpyridine) (S4VP diblock) copolymers with varying molecular weights and block compositions were synthesized via anionic polymerization, and their order−disorder transition temperatures (TODTs) were determined using oscillatory shear rheometry and small-angle X-ray scattering (SAXS). It has been found that for comparable molecular weight and block composition the TODT of S4VP diblock copolymer is exceedingly high compared with that of S2VP diblock copolymer. The experimental observation has been confirmed by theoretical predictions from currently held mean-field theory. For the theoretical predictions, temperature-dependent interaction parameters for the polystyrene (PS)/poly(2-vinylpyridine) (P2VP) pair and the PS/poly(4-vinylpyridine) (P4VP) pair were determined from SAXS profiles obtained at varying temperatures ranging from 125 to 185 °C for a low-molecular-weight (LMW) S2VP diblock copolymer and ranging from 160 to 195 °C for an LMW S4VP diblock copolymer and curve fitting to the Leibler theory. The molecular weights of LMW S2VP and LMW S4VP diblock copolymers employed were 10 200 and 2720, respectively, enabling us to obtain SAXS profiles in the disordered state of the respective block copolymers. The temperature-dependent specific volumes of PS, P2VP, and P4VP were determined at temperatures ranging from 25 to 200 °C using spectroscopic ellipsometry. To find the origin of the experimentally observed difference in TODT between S2VP and S4VP diblock copolymers, the thermally stimulated current method and dielectric relaxation spectroscopy were employed to investigate differences in polarizability between S2VP and S4VP diblock copolymers. It is concluded that much higher TODT of S4VP diblock copolymers as compared with the TODT of S2VP diblock copolymers is attributable to the stronger polarizability of P4VP in S4VP diblock copolymer compared with the polarizability of P2VP in S2VP diblock copolymer.
White organic light-emitting devices with a solution-processed and molecular host-employed emission layer (2005)

The brightness, efficiency, chromaticity, and processibility of white organic light-emitting devices have been markedly improved by forming therein via spin-coating a single white emission layer, in which the red, green, and blue dyes of iridium-based complexes were previously solution-mixed into the host matrix composed of small molecules instead of polymers. Among the hosts studied, the 4,4′-bis(carbazol-9-yl)-biphenyl-based devices performed best in terms of luminance and efficiency for having the lowest-energy barrier for electrons to inject from the hole-blocking layer to the host layer. The device having a pure white emission of (0.34, 0.35) had a maximum power efficiency of 2.9lm∕W at an applicable luminance of 1680cd∕m2, while 5.6lm∕W at 550cd∕m2 for that of (0.34, 0.39).
Active Tamoxifen Metabolite Plasma Concentrations After Coadministration of Tamoxifen and the Selective Serotonin Reuptake Inhibitor Paroxetine (2003)

Endoxifen is an active tamoxifen metabolite that is generated via CYP3A4-mediated N-demethylation and CYP2D6-mediated hydroxylation. Coadministration of paroxetine decreased the plasma concentration of endoxifen. Our data suggest that CYP2D6 genotype and drug interactions should be considered in women treated with tamoxifen.
Procedures to characterize and study P2Z/P2X7 purinoceptor: flow cytometry as a promising practical, reliable tool (2000)

The expression of P2Z/P2X7 purinoceptor in different cell types is well established. This receptor is a member of the ionotropic P2X receptor family, which is composed by seven cloned receptor subtypes (P2X1 - P2X7). Interestingly, the P2Z/P2X7 has a unique feature of being linked to a non-selective pore which allows the passage of molecules up to 900 Da depending on the cell type. Early studies of P2Z/P2X7 purinoceptor were exclusively based on classical pharmacological studies but the recent tools of molecular biology have enriched the analysis of the receptor expression. The majority of assays and techniques chosen so far to study the expression of P2Z/P2X7 receptor explore directly or indirectly the effects of the opening of P2Z/P2X7 linked pore. In this review we describe the main techniques used to study the expression and functionality of P2Z/P2X7 receptor. Additionally, the increasing need and importance of a multifunctional analysis of P2Z/P2X7 expression based on flow cytometry technology is discussed, as well as the adoption of a more complete analysis of P2Z/P2X7 expression involving different techniques.
Cellular Cholesterol Efflux Mediated by Cyclodextrins (1995)

In this study, we compared cholesterol efflux mediated by either high density lipoproteins (HDL3) or beta-cyclodextrins, cyclic oligosaccharides that are able to dissolve lipids in their hydrophobic core. beta-Cyclodextrin, 2-hydroxypropyl-beta-cyclodextrin, and methyl-beta-cyclodextrin at 10 mM induced the release of 50-90% of L-cell [3H]cholesterol after 8 h of incubation, with a major portion of this cholesterol being released in the first 1-2 h of incubation. The cholesterol efflux kinetics are different if cells are incubated with HDL3, which induces a relatively constant rate of release of cholesterol throughout an 8-h incubation. Cholesterol efflux to cyclodextrins was much greater than phospholipid release. To test the hypothesis that maximal efflux rate constants for a particular cell are independent of the type of acceptor, we estimated the maximal rate constants for efflux (Vmax) of cellular cholesterol from L-cells, Fu5AH cells, and GM3468A fibroblasts. The rate constant for HDL3-mediated efflux varied among cell lines in the order Fu5AH > L-cells > fibroblasts. However, these differences were not evident when cyclodextrins were used as cholesterol acceptors. The estimated Vmax values for cyclodextrin-mediated efflux were 3.5-70-fold greater than for HDL3 for the three cell lines. The very high efficiency of cyclodextrins in stimulating cell cholesterol efflux suggests that these compounds can be used in two general ways for studies of atherosclerosis: 1) as research tools to probe mechanisms of cholesterol transport and aspects of membrane structure or 2) as potential pharmacological agents that could modify in vivo cholesterol metabolism and influence the development of the atherosclerotic plaque.
An All‐Inorganic, Stable, and Highly Active Tetraruthenium Homogeneous Catalyst for Water Oxidation (2008)

Oxidation ohne Organik: Ein Tetraruthenium-Polyoxometallat-Hybrid (siehe Bild; Ru blau, O rot, Si gelb, W schwarz) katalysiert die schnelle Oxidation von H2O zu O2 bei Raumtemperatur. Der unter den Reaktionsbedingungen stabile Komplex wurde unter anderem kristallographisch und cyclovoltammetrisch charakterisiert.
The significance of polyunsaturated fatty acids in cutaneous biology (1996)

The skin epidermis displays a highly active metabolism of polyunsaturated fatty acids (PUFA). Dietary deficiency of linoleic acid (LA) and 18-carbon (n-6) PUFA results in characteristic scaly skin disorder and excessive epidermal water loss. Arachidonic acid, a 20-carbon (n-6) PUFA is metabolized via the cyclooxygenase pathway into predominantly prostaglandin E2 (PGE2) PGF2 alpha, and PGD2 and via the lipoxygenase pathway into predominantly 15-hydroxyeicosatetraenoic acid (15-HETE). The prostaglandins modulate normal skin physiological processes at low concentrations and inflammatory reactions at high concentrations. Similarly, the very active epidermal 15-lipoxygenase transforms dihomogammalinolenic acid (DGLA) into 15-hydroxy eicosatrienoic acid (15-HETrE), eicosapentaenoic acid (EPA) into 15-hydroxyeicosapentaenoic acid (15-HEPE) and docosahexaenoic acid (DHA) into 17-hydroxydocosahexaenoic acid (17-HDoHE), respectively. These monohydroxy acids exhibit anti-inflammatory properties. In contrast, the 18-carbon (n-6) PUFA is transformed into 13-hydroxy-9,11-octadecadienoic acid (13-HODE), which exerts antiproliferative properties in the tissue. Thus, the supplementation of diets with appropriate purified vegetable oils and/or fish oil may generate local cutaneous anti-inflammatory metabolites which could serve as a less toxic in vivo monotherapy or as adjuncts to standard therapeutic regimens for the management of skin inflammatory disorders.
Population-Based Incremental Learning: A Method for Integrating Genetic Search Based Function Optimization and Competitive Learning (1994)

Genetic algorithms (GAs) are biologically motivated adaptive systems which have been used, with varying degrees of success, for function optimization. In this study, an abstraction of the basic genetic algorithm, the Equilibrium Genetic Algorithm (EGA), and the GA in turn, are reconsidered within the framework of competitive learning. This new perspective reveals a number of different possibilities for performance improvements. This paper explores popula-tion-based incremental learning (PBIL), a method of combining the mechanisms of a genera-tional genetic algorithm with simple competitive learning. The combination of these two methods reveals a tool which is far simpler than a GA, and which out-performs a GA on large set of optimization problems in terms of both speed and accuracy. This paper presents an empirical analysis of where the proposed technique will outperform genetic algorithms, and describes a class of problems in which a genetic algorithm may be able to perform better. Extensions to this algorithm are discussed and analyzed. PBIL and extensions are compared with a standard GA on twelve problems, including standard numerical optimization func-tions, traditional GA test suite problems, and NP-Complete problems.
Cyclooxygenase-2 Inhibitors in Tumorigenesis (Part I) (1998)

The rate-limiting enzyme in arachidonate metabolism is mediated by enzymes known as cyclooxygenases (COXs). These enzymes catalyze the biosynthesis of prostaglandin H2, the precursor of molecules, such as prostaglandins, prostacyclin, and thromboxanes. The COX enzyme family consists of the classical COX-1 enzyme, which is constitutively expressed in many tissues, and a second enzyme, i.e., COX-2, which is induced by various stimuli, such as mitogens and cytokines, and is involved in many inflammatory reactions. Because nonsteroidal anti-inflammatory drugs inhibit both COX-1 and COX-2, these drugs also cause unwanted side effects, exemplified by gastrointestinal bleeding. Accumulating evidence indicates that nonsteroidal anti-inflammatory drugs can reduce the incidence of colorectal cancers in human and experimental animals and can reduce the polyp number and size in patients with familial adenomatous polyposis. This Part I (of a two-part review) focuses on the discovery of the COXs; their biochemical, molecular, and structural properties; and on the discovery of isozyme-specific inhibitors of COX activity.
Small Molecule RPE65 Antagonists Limit the Visual Cycle and Prevent Lipofuscin Formation (2005)

The accumulation of the lipofuscin fluorophores in retinal pigment epithelial (RPE) cells leads to the blinding degeneration characteristic of Stargardt disease and related forms of macular degeneration. RPE lipofuscin, including the fluorophore A2E, forms in large part as a byproduct of the visual cycle. Inhibiting visual cycle function with small molecules is required to prevent the formation of the retinotoxic lipofuscins. This in turn requires identification of rate-limiting steps in the operation of the visual cycle. Specific, non-retinoid isoprenoid compounds are described here, and shown through in both in vitro and in vivo experiments, to serve as antagonists of RPE65, a protein that is essential for the operation of the visual cycle. These RPE65 antagonists block regeneration of 11-cis-retinal, the chromophore of rhodopsin, thereby demonstrating that RPE65 is at least partly rate-limiting in the visual cycle. Furthermore, chronic treatment of a mouse model of Stargardt disease with the RPE65 antagonists abolishes the formation of A2E. Thus, RPE65 is also on the rate-limiting pathway to A2E formation. These nontoxic isoprenoid RPE65 antagonists are candidates for the treatment of forms of macular degeneration wherein lipofuscin accumulation is an important risk factor. These antagonists will also be used to probe the molecular function of RPE65 in vision.
ELF5-enforced transcriptional networks define an epigenetically regulated trophoblast stem cell compartment in the human placenta (2010)

The first definitive cell fate decision in development occurs at the blastocyst stage with establishment of the trophoblast and embryonic cell lineages. In the mouse, lineage commitment is achieved by epigenetic regulation of a critical gatekeeper gene, the transcription factor Elf5, that reinforces placental cell fate and is necessary for trophoblast stem (TS) cell self-renewal. In humans, however, the epigenetic lineage boundary seems to be less stringent since human embryonic stem (ES) cells, unlike their murine counterparts, harbour some potential to differentiate into trophoblast derivatives. Here, we show that ELF5 is expressed in the human placenta in villous cytotrophoblast cells but not in post-mitotic syncytiotrophoblast and invasive extravillous cytotrophoblast cells. ELF5 establishes a circuit of mutually interacting transcription factors with CDX2 and EOMES, and the highly proliferative ELF5(+)/CDX2(+) double-positive subset of cytotrophoblast cells demarcates a putative TS cell compartment in the early human placenta. In contrast to placental trophoblast, however, ELF5 is hypermethylated and largely repressed in human ES cells and derived trophoblast cell lines, as well as in induced pluripotent stem cells and murine epiblast stem cells. Thus, these cells exhibit an embryonic lineage-specific epigenetic signature and do not undergo an epigenetic reprogramming to reflect the trophoblast lineage at key loci such as ELF5. Our identification of the trophoblast-specific transcriptional circuit established by ELF5 will be instrumental to derive human TS cell lines that truly reflect early placental trophoblast and that will be most beneficial to gain insights into the aetiology of common pregnancy complications, including intra-uterine growth restriction and pre-eclampsia.
An Essential Role for Protein Phosphatases in Hippocampal Long-Term Depression (1993)

The effectiveness of long-term potentiation (LTP) as a mechanism for information storage would be severely limited if processes that decrease synaptic strength did not also exist. In area CA1 of the rat hippocampus, prolonged periods of low-frequency afferent stimulation elicit a long-term depression (LTD) that is specific to the stimulated input. The induction of LTD was blocked by the extracellular application of okadaic acid or calyculin A, two inhibitors of protein phosphatases 1 and 2A. The loading of CA1 cells with microcystin LR, a membrane-impermeable protein phosphatase inhibitor, or calmodulin antagonists also blocked or attenuated LTD. The application of calyculin A after the induction of LTD reversed the synaptic depression, suggesting that phosphatase activity is required for the maintenance of LTD. These findings indicate that the synaptic activation of protein phosphatases plays an important role in the regulation of synaptic transmission.
Peptabody‐EGF: A novel apoptosis inducer targeting ErbB1 receptor overexpressing cancer cells (2006)

The epidermal growth factor receptor (EGFR) plays a central role in cell life by controlling processes such as growth or proliferation. This receptor is commonly overexpressed in a number of epithelial malignancies and its upregulation is often associated with an aggressive phenotype of the tumor. Thus, targeting of EGFR represents a very promising challenge in oncology, and antibodies raised against this receptor have been investigated as potential antitumor agents. Various putative mechanisms of action were proposed for such antibodies, including decreased proliferation, induction of apoptosis, stimulation of the immunological response against targeted cancer cells or combinations thereof. We report here the development of an alternative high affinity molecule that is directed against EGFR. Production of this pentameric protein, named peptabody-EGF, includes expression in a bacterial expression system and subsequent refolding and multimerization of peptabody monomers. The protein complex contains 5 human EGF ligand domains, which confer specific binding towards the extracellular portion of EGFR. Receptor binding of the peptabody-EGF had a strong antiproliferative effect on different cancer cell lines overexpressing EGFR. However, cells expressing constitutive levels of the target receptor were barely affected. Peptabody-EGF treated cancer cells exhibited typical characteristics of apoptosis, which was found to be induced within 30 min after the addition of the peptabody-EGF. In vitro experiments demonstrated a significantly higher binding activity for peptabody-EGF than for the therapeutic monoclonal EGFR antibody Mab-425. Furthermore, the antitumor action provoked by the peptabody-EGF was greatly superior than antibody mediated effects when tested on EGFR overexpressing cancer cell lines. These findings suggest a potential application of this high affinity molecule as a novel tool for anti-EGFR therapy.
Anonymizing bipartite graph data using safe groupings (2008)

Private data often comes in the form of associations between entities, such as customers and products bought from a pharmacy, which are naturally represented in the form of a large, sparse bipartite graph. As with tabular data, it is desirable to be able to publish anonymized versions of such data, to allow others to perform ad hoc analysis of aggregate graph properties. However, existing tabular anonymization techniques do not give useful or meaningful results when applied to graphs: small changes or masking of the edge structure can radically change aggregate graph properties. We introduce a new family of anonymizations, for bipartite graph data, called ( k, l )-groupings. These groupings preserve the underlying graph structure perfectly, and instead anonymize the mapping from entities to nodes of the graph. We identify a class of "safe" ( k, l )-groupings that have provable guarantees to resist a variety of attacks, and show how to find such safe groupings. We perform experiments on real bipartite graph data to study the utility of the anonymized version, and the impact of publishing alternate groupings of the same graph data. Our experiments demonstrate that ( k, l )-groupings offer strong tradeoffs between privacy and utility.
Comparison of Seven Commercial TaqMan Master Mixes and Two Real-Time PCR Platforms Regarding the Rapid Detection of Porcine DNA (2020)

A pig-specific real-time PCR assay based on the mitochondrial <i>ND5</i> gene was developed to detect porcine material in food and other products. To optimize the performance of assay, seven commercial TaqMan master mixes and two real-time PCR platforms (Applied Biosystems StepOnePlus and Bio-rad CFX Connect) were used to evaluate the limit of detection (LOD) as well as the PCR efficiency and specificity. The LODs and PCR efficiencies for the seven master mixes on two platforms were 0.5-5 pg/reaction and 84.96%-108.80%, respectively. Additionally, non-specific amplifications of DNA from other animal samples (human, dog, cow, and chicken) were observed for four master mixes. These results imply that the sensitivity and specificity of a real-time PCR assay may vary depending on master mix and platform used. The best combination of master mix and real-time PCR platform can accurately detect 0.5 pg porcine DNA, with a PCR efficiency of 100.49%.
Endothelial Cytotoxicity Mediated by Serum Antibodies to Heat Shock Proteins of Escherichia coli and Chlamydia pneumoniae (1999)

Serum antibodies against HSP65/60 cross-react with human HSP60, cHSP60, and GroEL; correlate with the presence of antibodies to C pneumoniae and endotoxin; and mediate endothelial cytotoxicity. These findings suggest that humoral immune reactions to bacterial HSPs, such as cHSP60 and GroEL, may play an important role in the process of vascular endothelial injury, which is believed to be a key event in the pathogenesis of atherosclerosis.
Conversation as Planned Behavior (1980)

In this paper, planning models developed in artificial intelligence are applied to the kind of planning that must be carried out by participants in a conversation. A planning mechanism is defined, and a short fragment of a free‐flowing videotaped conversation is described. The bulk of the paper is then devoted to an attempt to understand the conversation in terms of the planning mechanism. This microanalysis suggests ways in which the planning mechanism must be augmented, and reveals several important conversational phenomena that deserve further investigation.
Contrast media reactions: experimental evidence against the allergy theory (1984)

In order to clarify whether adverse reactions to radiographic contrast media may be allergic in nature, it is essential to show that contrast media can act as haptens and induce antibody formation in vivo. Experiments in Hooded Lister rats have been carried out using techniques specifically designed to produce favourable conditions for the production of antibodies. No evidence of any antibody formation was found in any of the experiments. These experiments suggest that radiographic contrast media in their native form do not act as haptens, contrary to previously published data, and it therefore seems unlikely that the majority of adverse reactions to radiographic contrast media are allergic in nature.
[The effect of new synthetic cholesterol derivatives on cholesterol metabolism in cultured rabbit hepatocytes]. (1993)

The effects of three novel synthetic derivatives of cholesterol with ethoxy (I), aminoethoxy (II), azidoethoxy (III) and toluenesulfonyloxyethoxy (IV) groups in the 3 beta-hydroxy position of cholesterol on cholesterol synthesis as well as on apolipoprotein B and bile acid secretion in cultured rabbit hepatocytes have been studied. 3 beta-(2-hydroxyethoxy)-cholest-5-en (I) was used as a standard. It was found that the inhibiting effect of these compounds on cholesterol synthesis depends on their structure. Compound II (1 microgram/ml), which inhibited acetate incorporation into cholesterol by 30-50%, appeared to be the most effective among the other compounds tested. This derivative had no effect on the production of bile acids. Compound III was less effective, while compound IV had no effect on cholesterol synthesis. All the compounds under study reduced by 20-36% the secretion of the total apolipoprotein B as measured by the enzyme-linked immunosorbent assay (ELISA). None of the synthetic cholesterol derivatives influenced the leucine incorporation into the total protein fraction. The results obtained indicate that 3 beta-(2-aminoethoxy)cholest-5-en, the most effective compound among other cholesterol derivatives tested in the study, can serve as a basis for synthesizing novel cholesterol derivatives able to inhibit cholesterol biosynthesis in liver cells and to decrease the secretion of very low density lipoproteins in cultured rabbit hepatocytes.