gebhart/patrepeval huggingface dataset:
Transmural myocardial infarction in the absence of coronary arterial luminal narrowing in a young man with single coronary arterial anomaly (1978)

Reported is a case of single coronary arterial anomaly (SCAA) with its origin from the right sinus of Valsalva as demonstrated by selective coronary arteriography. No branches coursed between the great vessels. Electrocardiography and vectorcardiography were consistent with inferior wall myocardial infarction, although subsequent coronary arteriograms were free of luminal narrowing. While this is an infrequently recognized isolated coronary anomaly, it may carry significant risk of myocardial ishcemia. This risk may not depend alone upon the passage of a branch of SCAA between the great vessels. Evidence of precocious ischemic heart disease should alert the angiographer to look for anomalous coronary anatomy.
Carbon Nanotube Free-Standing Membrane of Pt/SWNTs as Catalyst Layer in Hydrogen Fuel Cells (2007)

A simple and promising fuel-cell architecture is demonstrated using a carbon nanotube free-standing membrane (CNTFSM) made from Pt supported on purified single-walled carbon nanotubes (Pt/SWNT), which act as the catalyst layer in a hydrogen proton exchange membrane fuel cell without the need for Nafion in the catalyst layer. The CNTFSM made from Pt/SWNT at a loading of 0.082 mg Pt cm–2 exhibits higher performance with a peak power density of 0.675 W cm–2 in comparison with a commercially available E-TEK electrocatalyst made of Pt supported on XC-72 carbon black, which had a peak power density of 0.395 W cm–2 at a loading of 0.084 mg Pt cm–2 also without Nafion in the catalyst layer.
The Use of Biomaterials in Islet Transplantation (2011)

Pancreatic islet transplantation is a therapeutic option to replace destroyed β cells in autoimmune diabetes. Islets are transplanted into the liver via the portal vein; however, inflammation, the required immunosuppression, and lack of vasculature decrease early islet viability and function. Therefore, the use of accessory therapy and biomaterials to protect islets and improve islet function has definite therapeutic potential. Here we review the application of niche accessory cells and factors, as well as the use of biomaterials as carriers or capsules, for pancreatic islet transplantation.
Structure-Guided Identification of C3d Residues Essential for Its Binding to Complement Receptor 2 (CD21) (2000)

A vital role for complement in adaptive humoral immunity is now beyond dispute. The crucial interaction is that between B cell and follicular dendritic cell-resident complement receptor 2 (CR2, CD21) and its Ag-associated ligands iC3b and C3dg, where the latter have been deposited as a result of classical pathway activation. Despite the obvious importance of this interaction, the location of a CR2 binding site within C3d, a proteolytic limit fragment of C3dg retaining CR2 binding activity, has not been firmly established. The recently determined x-ray structure of human C3d suggested a candidate site that was remote from the site of covalent attachment to Ag and consisted of an acidic residue-lined depression, which accordingly displays a significant electronegative surface potential. These attributes were consistent with the known ionic strength dependence of the CR2-C3d interaction and with the fact that a significant electropositive surface was apparent in a modeled structure of the C3d-binding domains of CR2. Therefore, we have performed an alanine scan of all of the residues within and immediately adjacent to the acidic pocket in C3d. By testing the mutant iC3b molecules for their ability to bind CR2, we have identified two separate clusters of residues on opposite sides of the acidic pocket, specifically E37/E39 and E160/D163/I164/E166, as being important CR2-contacting residues in C3d. Within the second cluster even single mutations cause near total loss of CR2 binding activity. Consistent with the proposed oppositely charged nature of the interface, we have also found that removal of a positive charge immediately adjacent to the acidic pocket (mutant K162A) results in a 2-fold enhancement in CR2 binding activity.
기술제휴의 동기가 하이테크(High-Tech) 기업의 기술제휴 파트너 선정기준, 거버넌스 및 성과에 미치는 영향에 관한 연구: 거래비용이론과 자원준거이론의 통합적 관점을 중심으로 (2013)

본 연구는 기술제휴 동기의 주요 이론적 배경인 거래비용이론(TCE)과 자원준거이론(RBV)의 기술제휴 파트너 선정기준, 거버넌스, 성과에 대한 설명력을 분석하였다. 기술제휴의 동기인 TCE와 RBV의 통합 분석 관점에서 기술제휴의 각 단계, 즉 기술 제휴 파트너 선정기준, 거버넌스, 성과에 미치는 영향을 측정하였다. 실증연구 결과, 국내 하이테크(High-Tech) 기업의 기술제휴 파트너 선정 시 TCE 관점과RBV 관점이 복합적으로 영향을 미치는 것으로 나타났으며, 거버넌스 유형 선택에는 TCE 관점이 보다 설명력이 있는 것으로 나타났다. 기술제휴의 성과에는 TCE 관점과 RBV 관점모두 관계가 있지만 RBV 관점의 설명력이 다소 큰 것으로 나타났다. 기술제휴 각 단계에 영향을 미치는 양 이론의 세부 변수를 제시하고, 영향을 미치는 원인을 분석하여 제시하였다. 본 연구의 결론에서는 연구결과에 대한 요약과 시사점 및 한계점에 대하여 기술하였다
Sleep Suppression after Basal Forebrain Lesions in the Cat (1968)

Large bilateral preoptic lesions produced complete sleeplessness in two cats. In eight additional cats, similar but smaller lesions resulted in a significant reduction of quiet (slow-wave) sleep by 55 to 73 percent, and active (paradoxical) sleep by 80 to 100 percent. These values were determined by pre- and postlesion 22-hour continuous observations. Complete sleeplessness was followed by lethal exhaustion within a few days, whereas incomplete sleeplessness persisted at maximum levels for 2 to 3 weeks. The suppression of sleep was characterized by a gradual onset during the first 1 to 2 weeks, and a complete or partial recovery after 6 to 8 weeks. The severity of sleep suppression was found to be related to the size and localization of lesions placed specifically within the preoptic area and not to transient disturbances in feeding and temperature regulation.
ENANTIOSELECTIVE CONJUGATE ADDITION REACTION MEDIATED BY CHIRAL LIGANDS (1985)

Abstract Chiral ligand mediated enantioselective conjugate addition reaction of lithiated dithioacetal derivative with prochiral α,β-unsaturated ester gives the corresponding adduct in 67% enantiomeric excess.
Identification of Stream Dispersion Coefficients by Adjoint Sensitivity Method (1999)

A numerical technique is presented for identification of spatially distributed dispersion coefficients for 2D mass transport in vertically mixed rivers and estuaries. The method is based on a nonlinear optimization procedure that minimizes the squared error between observed and computed concentration values. Gradient information is efficiently computed by the adjoint sensitivity method, so that the same effort is required regardless of the number of parameters involved. The method's limitations are presented, and the conditions of identifiability of the dispersion coefficients are investigated. Numerical tests on complex bathymetric configurations are presented to provide guidelines for the optimum location of monitoring stations. A large-scale application is presented corresponding to the upper Potomac estuary.
In vitro and in vivo effects of leukotriene B4 antagonism in a primate model of asthma. (1996)

To test the hypothesis that leukotriene (LT) B4 antagonists may be clinically useful in the treatment of asthma, CP-105,696 was evaluated in vitro, using chemotaxis and flow cytometry assays, and in vivo, using a primate asthma model. CP-105,696 inhibited LTB4-mediated monkey neutrophil chemotaxis (isolated cells, LTB4 = 5 nM) and CD11b upregulation (whole blood, LTB4 = 100 nM) with IC50 values of 20 nM and 16.5 microM, respectively. Using a modification of a previously described in vivo protocol (Turner et al. Am. J. Respir. Crit. Care Med. 1994. 149: 1153-1159), we observed that treatment with CP-105,696 inhibited the acute increase in bronchoalveolar lavage (BAL) levels of IL-6 and IL-8 by 56.9 +/- 13.2% and 46.9 +/- 14.5%, respectively, 4 h after challenge with Ascaris suum antigen (Ag). CP-105,696 tended to reduce the increase in BAL protein levels 0.5 h after Ag challenge by 47.5 +/- 18.3%, but this was not statistically significant. In addition, CP-105,696 prevented the significant 11-fold increase in airway responsiveness to methacholine after multiple Ag challenge. These results suggest that LTB4 partially mediates acute and chronic responses to antigen in an experimental primate asthma model and support the clinical evaluation of LTB4 antagonists in human asthma.
Food-derived serotonergic modulators: effects on mood and cognition (2013)

The most frequently described drugs in the treatment of mood disorders are selective serotonin reuptake and monoamine oxidase (MAO) inhibitors, enhancing serotonin levels in the brain. However, side-effects have been reported for these drugs. Because serotonin levels in the brain are dependent on the availability of the food-derived precursor tryptophan, foods such as chicken, soyabeans, cereals, tuna, nuts and bananas may serve as an alternative to improve mood and cognition. Here we discuss the effects of high- or low-tryptophan-containing food, as well as plant extracts with a modest monoamine reuptake and MAO-A inhibition functional profile, on mood and cognition in healthy and vulnerable human subjects and rodents. Together the studies suggest that there is an inverted U-shaped curve for plasma tryptophan levels, with low and too high tryptophan levels impairing cognition, and moderate to high tryptophan levels improving cognition. This relationship is found for both healthy and vulnerable subjects. Whereas this relationship may also exist for mood, the inverted U-shaped curve for plasma tryptophan levels and mood may be based on different tryptophan concentrations in healthy v. vulnerable individuals. Animal studies are emerging and allow further understanding of effects and the mode of action of food-derived serotonergic components on mood, cognition and mechanisms. Ultimately, insight into the concentrations of tryptophan and other serotonergic components in food having beneficial effects on mood and cognition in healthy, but particularly vulnerable, subjects may support well-being in our highly demanding society.
National Health Service Nursing Homes Again (1991)

Journal Article National Health Service Nursing Homes Again Get access JOHN BOND JOHN BOND Centre for Health Services Research University of Newcastle upon Tyne21 Claremont Place, Newcastle upon Tyne NE2 4AA Search for other works by this author on: Oxford Academic PubMed Google Scholar Age and Ageing, Volume 20, Issue 5, September 1991, Pages 313–315, https://doi.org/10.1093/ageing/20.5.313 Published: 01 September 1991
A retroviral promoter is sufficient to convert proto-src to a transforming gene that is distinct from the src gene of Rous sarcoma virus. (1990)

The src genes of four natural isolates of avian sarcoma viruses differ from cellular proto-src in two genetic substitutions: the promoter of the cellular gene is replaced by a retroviral counterpart, and at least six codons from the 3' terminus are replaced by retroviral or heterologous cell-derived elements. Since virus constructs with a complete proto-src coding region failed to transform avian cells but acquired transforming function by point mutations of various codons, it has been proposed that point mutation is sufficient to convert proto-src to a transforming gene. However, promoter substitution is sufficient to convert two other proto-onc genes, proto-ras and proto-myc, to retroviral transforming genes. In view of this, we have reexamined whether promoter substitution, point mutation, or both are necessary to convert proto-src into a retroviral transforming gene. It was found that a recombinant virus (RpSV), in which the src gene of Rous sarcoma virus (RSV) was replaced by the complete coding region of proto-src, transformed quail and chicken embryo cells. The oncogene of RpSV differs from the src gene of RSV in three genetic properties: (i) it is weaker--e.g., transformed cells are flatter; (ii) it is slower--e.g., focus formation takes 9 to 12 days compared to 4 days for RSV; and (iii) its host range is narrower than that of RSV--e.g., only subsets of heterogeneous embryo cells are transformed by RpSV even after weeks or months. Replacement of the proto-src 3' terminus of RpSV by that of src from RSV generates a recombinant virus (RpvSV) that equals RSV in transforming function. It is concluded that a retroviral promoter, naturally substituted via illegitimate recombination with retroviruses, is sufficient to convert at least three proto-onc genes, src, myc, and ras, to retroviral transforming genes.
Docking of Linear Peptide Antagonists into the Human V1a Vasopressin Receptor (1999)

A novel photoactivatable linear peptide antagonist selective for the V(1a) vasopressin receptor, [(125)I][Lys(3N(3) Phpa)(8)]HO-LVA, was synthesized, characterized, and used to photolabel the human receptor expressed in Chinese hamster ovary cells. Two specific glycosylated protein species at 85-90 and 46 kDa were covalently labeled, a result identical to that obtained with a previous photosensitive ligand, [(125)I]3N(3)Phpa-LVA (Phalipou, S., Cotte, N. , Carnazzi, E., Seyer, R., Mahe, E., Jard, S., Barberis, C., and Mouillac, B. (1997) J. Biol. Chem. 272, 26536-26544). To identify contact sites between the new photoreactive analogue and the V(1a) receptor, the labeled receptors were digested with Lys-C or Asp-N endoproteinases and chemically cleaved with CNBr. Fragmentation with CNBr, Lyc-C, and Asp-N used alone or in combination, led to the identification of a restricted receptor region spanning the first extracellular loop. The results established that sequence Asp(112)-Pro(120) could be considered as the smallest covalently labeled fragment with [(125)I][Lys(3N(3)Phpa)(8)]HO-LVA. Based on the present experimental result and on previous photoaffinity labeling data obtained with [(125)I]3N(3)Phpa-LVA (covalent attachment to transmembrane domain VII), three-dimensional models of the antagonist-bound receptors were constructed and then verified by site-directed mutagenesis studies. Strikingly, these two linear peptide antagonists, when bound to the V(1a) receptor, could adopt a pseudocyclic conformation similar to that of the cyclic agonists. Despite divergent functional properties, these peptide antagonists could interact with a transmembrane-binding site significantly overlapping that of the natural hormone vasopressin.
Outcome and One Year Follow-up of Intra-arterial Staphylokinase in 191 Patients with Peripheral Arterial Occlusion (2000)

Wild-type or equipotent variants of recombinant staphylokinase (rSak) were given intra-arterially (as a 2 mg bolus injection followed by an infusion of 1 mg/h or 0.5 mg/h overnight, with concomitant heparin [1000 IU/h]) to 191 patients of less than 80 years (62 +/- 1 years, mean +/- SEM), with a peripheral arterial occlusion (PAO) of less than 120 days (mean 14 +/- 1 days, median 11 days, 5 to 95 percentiles 3 to 30 days). Ninety nine patients presented with acute or subacute ischemia, 57 with severe claudication, 33 with chronic rest pain and 2 with gangrene. Occlusion occurred in 122 native arteries and in 69 grafts. Revascularization was complete in 83 percent (158/191), partial in 13 percent (24/191) and absent in 4 percent (7/191) after administration of 12 +/- 0.5 mg rSak over 14 +/- 0.7 h. Complete revascularization of acute occlusions of popliteal or more distal arteries was less frequent (60 percent, 15/25) than of acute occlusions of more proximal native arteries (95 percent, 37/39, p <0.001) or grafts (89 percent, 50/56, p = 0.005). Additional endovascular procedures were performed in 47 percent and subsequent elective bypass surgery in 23 percent of patients. Major bleeding occurred in 12 percent (23/191), one month mortality was 3.1 percent (6/191) and one year mortality was 6.9 percent (12/174). However, four patients (2.1 percent) had an intracranial bleeding following therapy: a 85 year old woman with severe diabetic arteriopathy, who was included in violation of the protocol, a 79 and a 74-year-old woman and a 74-year-old man, all with severe hypertension and limb threatening ischemia; these four patients died within two months after treatment. Amputations were performed within the first year in 16 of 162 surviving patients (9.8 percent): in 7 percent (7/96) with an occluded native artery and 14 percent (9/66) with an occluded graft (p = 0.19). No significant difference in lysis rate, one month mortality or one year amputation-free survival was observed in occlusions of recent onset (< or =14 days, n = 126) as compared to occlusions of longer duration (>14 days, n = 65). Treatment was interrupted prematurely in 4 patients because of a suspected allergic reaction. Fibrinogen levels remained unaffected during treatment (3.3 +/- 0.1 g/l before vs. 3.3 +/- 0.1 g/l after infusion, n = 167). In conclusion, rSak appears to be a highly effective thrombolytic agent in patients with PAO, resulting in a low one month mortality (3.1 percent) and a high one year amputation free survival (84 percent), with an acceptable incidence of major bleedings, but with occasional fatal intracranial hemorrhages.
Gene Regulation with Polyvalent siRNA−Nanoparticle Conjugates (2009)

We report the synthesis and characterization of polyvalent RNA-gold nanoparticle conjugates (RNA-Au NPs), nanoparticles that are densely functionalized with synthetic RNA oligonucleotides and designed to function in the RNAi pathway. The particles were rationally designed and synthesized to be free of degrading enzymes, have a high surface loading of siRNA duplexes, and contain an auxiliary passivating agent for increased stability in biological media. The resultant conjugates have a half-life six times longer than that of free dsRNA, readily enter cells without the use of transfection agents, and demonstrate a high gene knockdown capability in a cell model.
PIB‐based polyurethanes. IV. The morphology of polyurethanes containing soft co‐segments* (2009)

Abstract Novel polyurethanes consisting of polyisobutylene (PIB)/poly(tetramethylene oxide) (PTMO) or PIB/poly(hexamethylene carbonate) (PC) soft co‐segments in combination with 4,4′‐methylene‐bis(cyclohexyl isocyanate)/1,6‐hexanediol, 1,4‐butanediol, or 1,6‐hexamethylene diamine hard segments exhibit excellent mechanical properties (upto 31 MPa tensile strength with 700% elongation) together with unprecedented oxidative/hydrolytic stability. A structural model of the morphology of these polyurethanes was developed that reflects this combination of properties. The key new elements of our model are H bridges between the PTMO and PC type soft and urethane hard segments, which compatibilize the soft and hard domains, and the presence of large quantities of chemically resistant PIB soft segments that protect the other oxidatively/hydrolytically vulnerable constituents. A variety of FTIR, DSC, SAXS, AFM, and DMTA experiments strongly support the proposed morphological model. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 6180–6190, 2009
Elevated Circulating Levels of Tumor Necrosis Factor in Severe Chronic Heart Failure (1990)

These findings indicate that circulating levels of tumor necrosis factor are increased in cachectic patients with chronic heart failure and that this elevation is associated with the marked activation of the renin-angiotensin system seen in patients with end-stage cardiac disease.
Autophosphorylation of the DNA-dependent protein kinase catalytic subunit is required for rejoining of DNA double-strand breaks (2002)

Nonhomologous end-joining (NHEJ) is the predominant pathway that repairs DNA double-strand breaks (DSBs) in mammalian cells. The DNA-dependent protein kinase (DNA-PK), consisting of Ku and DNA-PK catalytic subunit (DNA-PKcs), is activated by DNA in vitro and is required for NHEJ. We report that DNA-PKcs is autophosphorylated at Thr2609 in vivo in a Ku-dependent manner in response to ionizing radiation. Phosphorylated DNA-PKcs colocalizes with both gamma-H2AX and 53BP1 after DNA damage. Mutation of Thr2609 to Ala leads to radiation sensitivity and impaired DSB rejoining. These findings establish that Ku-dependent phosphorylation of DNA-PKcs at Thr2609 is required for the repair of DSBs by NHEJ.
Escape from the Acute Wolff-Chaikoff Effect Is Associated with a Decrease in Thyroid Sodium/Iodide Symporter Messenger Ribonucleic Acid and Protein (1999)

In 1948, Wolff and Chaikoff reported that organic binding of iodide in the thyroid was decreased when plasma iodide levels were elevated (acute Wolff-Chaikoff effect), and that adaptation or escape from the acute effect occurred in approximately 2 days, in the presence of continued high plasma iodide concentrations.We later demonstrated that the escape is attributable to a decrease in iodide transport into the thyroid, lowering the intrathyroidal iodine content below a critical inhibitory threshold and allowing organification of iodide to resume.We have now measured the rat thyroid sodium/iodide symporter (NIS) messenger RNA (mRNA) and protein levels, in response to both chronic and acute iodide excess, in an attempt to determine the mechanism responsible for the decreased iodide transport.Rats were given 0.05% NaI in their drinking water for 1 and 6 days in the chronic experiments, and a single 2000-g dose of NaI ip in the acute experiments.Serum was collected for iodine and hormone measurements, and thyroids were frozen for subsequent measurement of NIS, TSH receptor, thyroid peroxidase (TPO), thyroglobulin, and cyclophilin mRNAs (by Northern blotting) as well as NIS protein (by Western blotting).Serum T 4 and T 3 concentrations were significantly decreased at 1 day in the chronic experiments and returned to normal at 6 days, and were unchanged in the acute experiments.Serum TSH levels were unchanged in both paradigms.Both NIS mRNA and protein were decreased at 1 and 6 days after chronic iodide ingestion.NIS mRNA was decreased at 6 and 24 h after acute iodide administration, whereas NIS protein was decreased only at 24 h.TPO mRNA was decreased at 6 days of chronic iodide ingestion and 24 h after acute iodide administration.There were no iodide-induced changes in TSH receptor and thyroglobulin mRNAs.These data suggest that iodide administration decreases both NIS mRNA and protein expression, by a mechanism that is likely to be, at least in part, transcriptional.Our findings support the hypothesis that the escape from the acute Wolff-Chaikoff effect is caused by a decrease in NIS, with a resultant decreased iodide transport into the thyroid.The observed decrease in TPO mRNA may contribute to the iodine-induced hypothyroidism that is common in patients with Hashimoto's thyroiditis.
Single-pixel imaging via compressive sampling (2008)

The authors present a new approach to building simpler, smaller, and cheaper digital cameras that can operate efficiently across a broader spectral range than conventional silicon-based cameras. The approach fuses a new camera architecture based on a digital micromirror device with the new mathematical theory and algorithms of compressive sampling.