gebhart/patrepeval huggingface dataset:
Polishing with T4 orPfupolymerase increases the efficiency of cloning of PCR fragments (1994)

Journal Article Polishing with T4 or Pfu polymerase increases the efficiency of cloning of PCR fragments Get access Gina L. Costa, Gina L. Costa Stratagene Cloning Systems11099 North Torrey Pines Road, La Jolla, CA 92037, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Michael P. Weiner Michael P. Weiner * Stratagene Cloning Systems11099 North Torrey Pines Road, La Jolla, CA 92037, USA * To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 22, Issue 12, 25 June 1994, Page 2423, https://doi.org/10.1093/nar/22.12.2423 Published: 25 June 1994 Article history Received: 05 April 1994 Revision received: 09 May 1994 Accepted: 09 May 1994 Published: 25 June 1994
Different behavior of branched and linear polyethylenimine for gene deliveryin vitro andin vivo (2001)

Branched and linear PEI/DNA complexes differ in their ability to transfect cells. The greater efficiency of linear PEI might be due to an inherent kinetic instability under salt conditions. Understanding how to employ this kinetic instability of linear PEI could help in designing future vectors with greater flexibility and transfection efficiency in vivo.
Hydrogen-bonding self-assembly of multichromophore structures (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHydrogen-bonding self-assembly of multichromophore structuresPaolo Tecilla, Robert P. Dixon, Gregory Slobodkin, David S. Alavi, David H. Waldeck, and Andrew D. HamiltonCite this: J. Am. Chem. Soc. 1990, 112, 25, 9408–9410Publication Date (Print):December 1, 1990Publication History Published online1 May 2002Published inissue 1 December 1990https://pubs.acs.org/doi/10.1021/ja00181a061https://doi.org/10.1021/ja00181a061research-articleACS PublicationsRequest reuse permissionsArticle Views813Altmetric-Citations127LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Preparation and Organization of Nanoscale Polyelectrolyte‐Coated Gold Nanoparticles (2003)

Abstract The layer‐by‐layer (LbL) desposition of oppositely charged polyelectrolytes from adsorption solutions of different ionic strength onto ∼7 nm diameter carboxylic acid‐derivatized gold nanoparticles has been studied. The polyelectrolyte‐modified nanoparticles were characterized by UV‐vis spectrophotometry, microelectrophoresis, analytical ultracentrifugation, and transmission electron microscopy. UV‐vis data showed that the peak plasmon absorption wavelength of the gold nanoparticles red‐shifted after each adsorption step, and microelectrophoresis experiments revealed a reversal in the surface charge of the nanoparticles following deposition of each layer. These data are consistent with the formation of polyelectrolyte layers on the nanoparticles. Analytical ultracentrifugation showed an increase in mean nanoparticle diameter on adsorption of the polyelectrolytes, confirming the formation of gold‐core/polyelectrolyte‐shell nanoparticles. Transmission electron microscopy studies showed no signs of aggregation of the polyelectrolyte‐coated nanoparticles. The adsorption of the polyelectrolyte‐coated gold nanoparticles onto oppositely charged planar supports has also been examined. UV‐vis spectrophotometry and atomic force microscopy showed increased amounts of nanoparticles were adsorbed with increasing ionic strength of the nanoparticle dispersions. This allows control of the nanoparticle surface loading by varying the salt content in the nanoparticle dispersions used for adsorption. The LbL strategy used in this work is expected to be applicable to other nanoparticles (e.g., semiconductors, phosphors), thus providing a facile means for their controlled surface modification through polyelectrolyte nanolayering. Such nanoparticles are envisaged to have applications in the biomedical and bioanalytical fields, and to be useful building blocks for the creation of advanced nanoparticle‐based films.
Clostridium difficile infection in children hospitalized due to diarrhea (2014)

The frequency of Clostridium difficile infection (CDI)-related hospitalizations is increasing. The aim of this study was to determine the extent of CDI among children hospitalized with diarrhea, risk factors or predictors for severe CDI, the prevalence of NAP1, and to compare the course of CDI depending on bacteria toxicity profile. A retrospective analysis of case records of 64 children (age range 3 months- 16 years, median age 2.12 years) with CDI as defined by diarrheal disease and positive polymerase chain reaction (PCR) test (Xpert C. difficile) was conducted. Modified na- tional adult guidelines were usedtoassessthe severityofCDI. CDIs represented 2.7 % of patients with diarrhea (13.5 cases per 1,000 admissions). Thirty-three CDIs (52 %) were community-associated. Antibacterial use preceded CDI in 61 patients (95 %). Seventeen cases (27 %) were binary toxin- positive (CDT+), 13 of which were NAP1 (20.5 %). Over 75 % of CDIs with NAP1 was hospital-acquired, and more often proceeded with generalized infection (p<0.05). Risk factors for severe CDI (34 %) included NAP1 (odds ratio (OR), 4.85; 95 % confidence interval (Cl), 1.23, 21.86) and co-morbidities(OR, 4.25; 95% Cl,1.34, 14.38). Diarrhea ≥10 stools daily was associated with severe CDI (p=0.01). Recur- rence occurred in three patients (4.5 %). There was no mor- tality.C.difficile isanimportantfactorofantibiotic-associated diarrhea in children. Co-morbidities and NAP1 predispose to severe CDI.
Reply to Dr Yu et al: Inadvertent quadriceps weakness following the pericapsular nerve group (PENG) block (2019)

To the Editor, Since the publication of our case report on the pericapsular nerve group (PENG) block,[1][1] we are thrilled to have enthusiastic response from many different centers which reproduced excellent results from this procedure. We are delighted to read the letter from Dr Yu et al [2][2]
Identification of a natural soluble neuropilin-1 that binds vascular endothelial growth factor:In vivoexpression and antitumor activity (2000)

Neuropilin-1 (NRP1) is a 130-kDa transmembrane receptor for semaphorins, mediators of neuronal guidance, and for vascular endothelial growth factor 165 (VEGF(165)), an angiogenesis factor. A 2.2-kb truncated NRP1 cDNA was cloned that encodes a 644-aa soluble NRP1 (sNRP1) isoform containing just the a/CUB and b/coagulation factor homology extracellular domains of NRP1. sNRP1 is secreted by cells as a 90-kDa protein that binds VEGF(165), but not VEGF(121). It inhibits (125)I-VEGF(165) binding to endothelial and tumor cells and VEGF(165)-induced tyrosine phosphorylation of KDR in endothelial cells. The 3' end of sNRP1 cDNA contains a unique, 28-bp intron-derived sequence that is absent in full-length NRP1 cDNA. Using a probe corresponding to this unique sequence, sNRP1 mRNA could be detected by in situ hybridization differentially from full-length NRP1 mRNA, for example, in cells of liver, kidney, skin, and breast. Analysis of blood vessels in situ showed that NRP1, but not sNRP1, was expressed. sNRP1 was functional in vivo. Unlike control tumors, tumors of rat prostate carcinoma cells expressing recombinant sNRP1 were characterized by extensive hemorrhage, damaged vessels, and apoptotic tumor cells. These results demonstrate the existence of a naturally occurring, soluble NRP1 that is expressed differently from intact NRP1 and that appears to be a VEGF(165) antagonist.
Thalidomide and Analogs (1999)

Thalidomide was developed in 1958 by Chemie Grunenthal as an oral sedative. Subsequently, McBride (1) and Lenz (2) described the association between maternal usage of thalidomide and fetal limb defects. The effect of thalidomide as a teratogen was experimentally confirmed in a number of species (3–8). By the time thalidomide was withdrawn from the market in 1962, thousands of babies worldwide were estimated to have been born with thalidomide-related defects. As a consequence, the mechanism by which thalidomide causes birth defects has been intensively investigated. Nevertheless, the mechanism has remained elusive (9).
Caspase Substrates and Cellular Remodeling (2011)

The caspases are unique proteases that mediate the major morphological changes of apoptosis and various other cellular remodeling processes. As we catalog and study the myriad proteins subject to cleavage by caspases, we are beginning to appreciate the full functional repertoire of these enzymes. Here, we examine current knowledge about caspase cleavages: what kinds of proteins are cut, in what contexts, and to what end. After reviewing basic caspase biology, we describe the technologies that enable high-throughput caspase substrate discovery and the datasets they have yielded. We discuss how caspases recognize their substrates and how cleavages are conserved among different metazoan organisms. Rather than comprehensively reviewing all known substrates, we use examples to highlight some functional impacts of caspase cuts during apoptosis and differentiation. Finally, we discuss the roles caspase substrates can play in medicine. Though great progress has been made in this field, many important areas still await exploration.
Human immunodeficiency virus 1 reverse transcriptase (1989)

We have analyzed the kinetics of DNA synthesis catalyzed by reverse transcriptase from human immunodeficiency virus 1 (HIV-1). Reverse transcriptase, overproduced in Escherichia coli and purified to homogeneity, has polymerase and RNase H activity. Reverse transcriptase forms a stable complex with poly(rA).oligo(dT) primer-templates in the absence of Mg2+ and dTTP with an equilibrium dissociation constant of 3 nM. Synthesis from these preformed complexes can be initiated, and restricted to a single processive cycle, by the simultaneous addition of Mg2+, dTTP, and excess competitor RNA. Preformed complexes decay with a maximal half-life of 2-3 min. Synthesis on poly(rA) templates is processive with an incorporation rate of 10-15 nucleotides/s at 37 degrees C. Processivity varies widely with the template used, increasing from a few to greater than 300 nucleotides in the order: poly(dA) less than double-stranded DNA less than single-stranded DNA less than single-stranded RNA less than poly(rA). On double-stranded DNA reverse transcriptase catalyzes limited strand-displacement synthesis of up to 50 nucleotides. On RNA-DNA hybrids significant DNA synthesis is observed only after degradation of the RNA strand by the RNase H activity of reverse transcriptase. Intermolecular strand switching occurs with poly(rA) templates. At low ionic strength reverse transcriptase can use multiple templates with a single primer, leading to products of greater than template length. Reverse transcriptase and primer do not have to dissociate during the exchange of template strands, thus allowing processive DNA synthesis across template borders.
Duration of Breastfeeding and Risk of Overweight: A Meta-Analysis (2005)

Observational studies suggest a longer duration of breastfeeding to be associated dose dependently with a decrease in risk of overweight in later life. The authors performed a comprehensive meta-analysis of the existing studies on duration of breastfeeding and risk of overweight. Studies were included that reported the odds ratio and 95% confidence interval (or the data to calculate them) of overweight associated with breastfeeding and that reported the duration of breastfeeding and used exclusively formula-fed subjects as the referent. Seventeen studies met the inclusion criteria. By meta-regression, the duration of breastfeeding was inversely associated with the risk of overweight (regression coefficient=0.94, 95% confidence interval (CI): 0.89, 0.98). Categorical analysis confirmed this dose-response association (<1 month of breastfeeding: odds ratio (OR)=1.0, 95% CI: 0.65, 1.55; 1-3 months: OR=0.81, 95% CI: 0.74, 0.88; 4-6 months: OR=0.76, 95% CI: 0.67, 0.86; 7-9 months: OR=0.67, 95% CI: 0.55, 0.82; >9 months: OR=0.68, 95% CI: 0.50, 0.91). One month of breastfeeding was associated with a 4% decrease in risk (OR=0.96/month of breastfeeding, 95% CI: 0.94, 0.98). The definitions of overweight and age had no influence. These findings strongly support a dose-dependent association between longer duration of breastfeeding and decrease in risk of overweight.
State Action and the Enforcement of Compulsory Arbitration Agreements Against Employment Discrimination Claims (2000)

For the past few years, the federal courts of appeal have been struggling with the issue of whether employers may enforce compulsory arbitration clauses against employees who bring employment discrimination claims under Title VII or the Americans with Disabilities Act of 1990 ("ADA").Compulsory arbitration clauses require prospective employees, as a condition of obtaining employment, to sign a contract waiving their rights to a jury trial regarding all future causes of action.The debate in the federal courts thus far has focused on whether Congress intended in passing the Civil Rights Act of 1991 ("1991 Act"), which amended Title VII, and the ADA, to preclude the compulsory arbitration of Title VII and ADA claims.Section 118 of the 1991 Act and section 12212 of the ADA each state that "[w]here appropriate and to the extent authorized by law, the use of... arbitration, is encouraged to resolve disputes arising under...
Risk of Uveitis Among People With Psoriasis (2017)

People with severe psoriasis and those with mild psoriasis have an increased risk of uveitis. Clinicians may use this finding as a guide for uveitis risk stratification among patients with different inflammatory presentations on the spectrum of psoriatic disease.
Digital Adaptive IIP2 Calibration Scheme for CMOS Downconversion Mixers (2008)

This paper presents a cancellation scheme of second order intermodulation distortion based on adaptive digital calibration of the RF downconversion mixer. The system utilizes a low-complexity LMS-based equalizer, which correlates differential mode distortion with common mode distortion to estimate optimum settings of the analog IP2 calibration circuit. A tunable IQ mixer prototype, fabricated in a 0.13 mum RF CMOS technology and operating under low voltage supply of 1.5 V, is presented. Experimental results confirm the feasibility of the proposed IP2 calibration method.
31P magnetic resonance spectroscopy in dilated cardiomyopathy and coronary artery disease. Altered cardiac high-energy phosphate metabolism in heart failure. (1992)

Cardiac high-energy phosphate metabolism at rest is normal in LAD stenosis and chronic myocardial infarction in the absence of heart failure. The CP/ATP ratio has low specificity for the diagnosis of dilated cardiomyopathy. However, CP/ATP correlated with the clinical severity of heart failure and may improve during clinical recompensation.
Fibroblast Growth Factor-2 Selectively Stimulates Angiogenesis of Small Vessels in Arterial Tree (2000)

There is a critical need for quantifiable models of angiogenesis in vivo, and in general, differential effects of angiogenic regulators on vascular morphology have not been measured. Because the potent angiogenic stimulators fibroblast growth factor (FGF)-2 (basic FGF) and vascular endothelial growth factor (VEGF) are reported to stimulate angiogenesis through distinct signaling pathways, we hypothesized that FGF-2 stimulates vascular morphology differently than does VEGF and that stimulation of angiogenesis by FGF-2 is directly correlated to FGF receptor density. FGF-2 was applied at embryonic day 7 (E7), E8, or E9 to the quail chorioallantoic membrane (CAM); subsequent response of the arterial tree was measured by the fractal dimension (D(f)), a mathematical descriptor of complex spatial patterns, and by several generational branching parameters that included vessel length density (L(v)). After application of FGF-2 at E7, arterial density increased according to D(f) as a direct function of increasing FGF-2 concentration, and FGF-2 stimulated the growth of small vessels, but not of large vessels, according to L(v) and other branching parameters. For untreated control specimens at E7, L(v) of small vessels and D(f) were 11.1+/-1.6 cm(-1) and 1.38+/-0.01, respectively; at E8, after treatment with 5 microgram FGF-2/CAM for 24 hours, L(v) of small vessels and D(f) increased respectively to 22.8+/-0.7 cm(-1) and 1. 49+/-0.02 compared with 16.3+/-0.9 cm(-1) and 1.43+/-0.02 for PBS-treated control specimens. Application of FGF-2 at E8 and E9 did not significantly increase arterial density. By immunohistochemistry, the expression of 4 high-affinity tyrosine kinase FGF receptors was significantly expressed at E7, when CAM vasculature responded strongly to FGF-2 stimulation, but FGF receptor expression decreased throughout the CAM by E8, when vascular response to FGF-2 was negligible. In conclusion, the "fingerprint" vascular pattern elicited by FGF-2 was distinct from vascular patterns induced by other angiogenic regulators that included VEGF(165), transforming growth factor-beta1, and angiostatin.
Localization of the gangliosides GD2 and GD3 in adhesion plaques and on the surface of human melanoma cells. (1984)

The predominant gangliosides produced by two cultured human melanoma cell lines are GD3 and/or GD2. These gangliosides were found to be cell associated and present in substratum-attached material after cell removal by EDTA. Monoclonal antibodies directed to GD2 and GD3 specified the cell-surface distribution of these gangliosides and localized them in focal adhesion plaques at the interface of cells and their substratum. These attachment sites did not represent indiscriminant membrane fragments remaining after removal of cells with EDTA, because neither melanoma-associated proteoglycan nor class I histocompatibility antigens were detected by their respective antibodies. Our data suggest that the disialogangliosides GD2 and GD3 may be involved in the interaction between human melanoma cells and solid substrata.
A static type system for message passing (1991)

Much research has been performed with the aim of isolating the basic notions of object-oriented languages and of describing them by basic operators which can be embedded in strongly-typed languages. In this context we define an atomic linguistic construct to capture the notion of message passing, and we define a static and strong type system, based on subtyping, for this construct. Message passing is modelled as the application of an overloaded function, whose behavior is determined only at compile-time on the basis of the class which created a distinguished argument, the object “receiving ” the message. By embedding this mechanism in a data types we can obtain the full functionality of object-oriented languages. We show that this approach is strictly more expressive then the usual interpretation of message passing as selection plus application of a functional record field. 1.
Recent Perspectives in Ocular Drug Delivery (2008)

Anatomy and physiology of the eye makes it a highly protected organ. Designing an effective therapy for ocular diseases, especially for the posterior segment, has been considered as a formidable task. Limitations of topical and intravitreal route of administration have challenged scientists to find alternative mode of administration like periocular routes. Transporter targeted drug delivery has generated a great deal of interest in the field because of its potential to overcome many barriers associated with current therapy. Application of nanotechnology has been very promising in the treatment of a gamut of diseases. In this review, we have briefly discussed several ocular drug delivery systems such as microemulsions, nanosuspensions, nanoparticles, liposomes, niosomes, dendrimers, implants, and hydrogels. Potential for ocular gene therapy has also been described in this article. In near future, a great deal of attention will be paid to develop non-invasive sustained drug release for both anterior and posterior segment eye disorders. A better understanding of nature of ocular diseases, barriers and factors affecting in vivo performance, would greatly drive the development of new delivery systems. Current momentum in the invention of new drug delivery systems hold a promise towards much improved therapies for the treatment of vision threatening disorders.
Activation of CPT-11 in mice: identification and analysis of a highly effective plasma esterase. (2000)

The camptothecin prodrug CPT-11 (irinotecan, 7-ethyl-10-[4-(1-piperidino)-1-piperidino]carbonyloxycamptothecin) is converted by esterases to yield the potent topoisomerase I poison SN-38 (7-ethyl-10-hydroxycamptothecin). Recently, a mouse strain (Es1(e)) has been identified that demonstrates reduced plasma esterase activity, and we have monitored the ability of plasma from these mice to metabolize CPT-11. Total plasma esterase activity was reduced 3-fold in Esl(e)mice in comparison to control mice, and this resulted in a 200-fold reduction in SN-38 production after incubation with CPT-11 in vitro. In addition, pharmacokinetic studies of CPT-11 and SN-38 in these animals demonstrated approximately 5-fold less conversion to SN-38. However, extracts derived from tissues from Es1(e) animals revealed total esterase activities similar to those of control mice, and these extracts metabolized CPT-11 with equal efficiency. Northern analysis of RNA isolated from organs indicated that the liver was the primary source of Es-1 gene expression and that very low levels of Es-1 RNA were present in Es1(e) mice. These results suggest that the reduced levels of Es-1 esterase present in Es1(e) mice are due to down-regulation of gene transcription, and that this plasma esterase is responsible for the majority of CPT-11 metabolism in mice.
Mutagenic activity of antileprosy drugs and their derivatives. (1983)

We tested the mutagenic activity of antileprosy drugs (clofazimine, ethionamide, prothionamide, prothionamide-S-oxide, rifampin, and dapsone and many of its derivatives) using the Ames Salmonella/microsome assay system. None of these, including N-acetylated and N-hydroxylated derivatives of dapsone, were found to be positive with or without metabolic activation of this test. However, the sulfoxide and sulfide analogs of dapsone were found to be mutagenic with metabolic activation. These two analogs could not be detected in pharmaceutical preparations of dapsone (less than 0.01%), nor could they be found (in either unconjugated or conjugated form) in urine from volunteers taking a single oral dose of 50 mg of dapsone or from patients receiving daily oral doses of 100 mg of dapsone. Also, urine concentrates from volunteers taking 50 mg of dapsone did not exhibit mutagenic activity in the Ames screen. These results indicate that patients receiving antileprosy therapy with clofazimine, dapsone, ethionamide, prothionamide, and/or rifampin are not being exposed to mutagenic (and thereby possible carcinogenic) drugs.