gebhart/patrepeval huggingface dataset:
Heterogeneous Ion Exchange in a Flowing System (1944)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHeterogeneous Ion Exchange in a Flowing SystemHenry C. ThomasCite this: J. Am. Chem. Soc. 1944, 66, 10, 1664–1666Publication Date (Print):October 1, 1944Publication History Published online1 May 2002Published inissue 1 October 1944https://pubs.acs.org/doi/10.1021/ja01238a017https://doi.org/10.1021/ja01238a017research-articleACS PublicationsRequest reuse permissionsArticle Views3320Altmetric-Citations1358LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Physician acceptance of telemedicine technology: an empirical investigation. (1999)

Fast-growing interest in telemedicine and increased investment in its enabling technology have made physician technology acceptance a growing concern for development and management of telemedicine. At the dawn of large-scale technology implementation by health care organizations around the globe, it is essential to understand physicians' attitudes toward use of telemedicine technology and their intention to use the technology. In this study, we used Theory of Planned Behavior to investigate technology acceptance among physicians who practiced in public tertiary hospitals in Hong Kong. Our data supported the investigated theory and the results suggest that attitude and perceived behavioral control are crucial to physician technology acceptance. Overall, physicians showed positive attitudes toward use of telemedicine technology and exhibited moderate intention to use the technology, primarily for clinical purposes. Implications for development and management of telemedicine also are discussed.
Temperature dependence of anisotropic diffraction in holographic polymer-dispersed liquid crystal memory (2013)

Grating devices using photosensitive organic materials play an important role in the development of optical and optoelectronic systems. High diffraction efficiency and polarization dependence achieved in a holographic polymer-dispersed liquid crystal (HPDLC) grating are expected to provide polarization-controllable optical devices, such as a holographic memory for optically reconfigurable gate arrays (ORGAs). However, the optical property is affected by the thermal modulation around the transition temperature (T(ni)) where the liquid crystal (LC) changes from nematic to isotropic phases. The temperature dependence of the diffraction efficiency in HPDLC grating is investigated using four types of LC composites comprised of LCs and monomers having different physical properties such as T(ni) and anisotropic refractive indices. The holographic memory formed by the LC with low anisotropic refractive index and LC diacrylate monomer implements optical reconfiguration for ORGAs at a high temperature beyond T(ni) of LC.
Nanopore-Based Identification of Individual Nucleotides for Direct RNA Sequencing (2013)

We describe a label-free ribobase identification method, which uses ionic current measurement to resolve ribonucleoside monophosphates or diphosphates in α-hemolysin protein nanopores containing amino-cyclodextrin adapters. The accuracy of base identification is further investigated through the use of a guanidino-modified adapter. On the basis of these findings, an exosequencing approach is envisioned in which a processive exoribonuclease (polynucleotide phosphorylase) presents sequentially cleaved ribonucleoside diphosphates to a nanopore.
RNAi nanomedicines: challenges and opportunities within the immune system (2010)

RNAi, as a novel therapeutic modality, has an enormous potential to bring the era of personalized medicine one step further from notion into reality. However, delivery of RNAi effector molecules into their target tissues and cells remain extremely challenging. Major attempts have been made in recent years to develop sophisticated nanocarriers that could overcome these hurdles. This review will present the recent progress with the challenges and opportunities in this emerging field, focusing mostly on the in vivo applications with special emphasis on the strategies for RNAi delivery into immune cells.
Dose-related effects of psoralen and ultraviolet light on the cell cycle of murine melanoma cells. (1982)

Cloudman (S91) murine melanoma cells were treated with 4'-hydroxymethyltrioxsalen (HMT), a bifunctional psoralen and exposed to long-wavelength (365 nm) ultraviolet light. DNA content of the cells stained with propidium iodide was measured by flow cytometry, and cell cycle phases were delineated from the DNA histograms by using a curve-fitting routine. We found that HMT in combination with long-wavelength (365 nm) ultraviolet irradiation blocked melanoma cells in different phases of the cell cycle, depending on the dose of long-wavelength (365 nm) ultraviolet light and the concentration of HMT. The binding of [3H]HMT to DNA was measured parallel with cell cycle analyses. Treatments with HMT at concentrations corresponding to about 1 HMT bound per 10(6) base pairs of DNA led to the accumulation of cells with predominantly G2 DNA content. At higher concentrations (2 to 3 HMT/10(6) base pairs), the cells were blocked in the S and G1 phases. In conclusion, we have shown that extremely sparse substitution of HMT to DNA blocks melanoma cells in the G2 phase or other phases of the cell cycle in a dose-dependent manner.
IL-6/STAT3 promotes regeneration of airway ciliated cells from basal stem cells (2014)

The pseudostratified airway epithelium of the lung contains a balanced proportion of multiciliated and secretory luminal cells that are maintained and regenerated by a population of basal stem cells. However, little is known about how these processes are modulated in vivo, and about the potential role of cytokine signaling between stem and progenitor cells and their niche. Using a clonal 3D organoid assay, we found that IL-6 stimulated, and Stat3 inhibitors reduced, the generation of ciliated vs. secretory cells from basal cells. Gain-of-function and loss-of-function studies with cultured mouse and human basal cells suggest that IL-6/Stat3 signaling promotes ciliogenesis at multiple levels, including increases in multicilin gene and forkhead box protein J1 expression and inhibition of the Notch pathway. To test the role of IL-6 in vivo genetically, we followed the regeneration of mouse tracheal epithelium after ablation of luminal cells by inhaled SO2. Stat3 is activated in basal cells and their daughters early in the repair process, correlating with an increase in Il-6 expression in platelet-derived growth factor receptor alpha(+) mesenchymal cells in the stroma. Conditional deletion in basal cells of suppressor of cytokine signaling 3, encoding a negative regulator of the Stat3 pathway, results in an increase in multiciliated cells at the expense of secretory and basal cells. By contrast, Il-6 null mice regenerate fewer ciliated cells and an increased number of secretory cells after injury. The results support a model in which IL-6, produced in the reparative niche, functions to enhance the differentiation of basal cells, and thereby acts as a "friend" to promote airway repair rather than a "foe."
In Vitro and In Vivo Variation in Transferrin Receptor Expression on a Human Medulloblastoma Cell Line (1995)

The poor prognosis associated with pediatric central nervous system tumors such as medulloblastoma has led to the development and investigation of a variety of new treatment techniques. Therapeutic agents include targeted-toxin conjugates or immunotoxins that show significant in vitro activity against many brain tumors. Transferrin receptors (TRs) are specific, cell-surface antigens that are expressed preferentially on brain tumors rather than on normal human brain tissue. This antigen has been successfully targeted in human and nonhuman brain tumors in vitro and in vivo. In this study, when TRs were used as a target in the DAOY human medulloblastoma-derived cell line in vitro, a significant level of expression was confirmed by testing the sensitivity to different immunotoxins. To ensure the relevance of the in vitro data to the in vivo situation, we also analyzed TR expression in DAOY tumors growing in athymic mice and rats. Immunocytochemistry, immunohistochemistry, immunobead binding, immunofluorescence, 125iodine-transferrin binding, and Northern blot analysis were used to compare TR expression in DAOY cells in vitro and in vivo. All in vitro assays demonstrated significant TR expression, whereas in vivo, the TR expression was negligible in the DAOY tissue. The results caution against extrapolating in vitro antigen and receptor expression data directly to the in vivo situation. Using a transferrin-toxin conjugate in a nude rat model of leptomeningeal carcinomatosis, we achieved therapeutic efficacy, despite demonstrating reduced TR expression on tumor tissue. With respect to clinical efficacy, the reduced expression of TR on DAOY medulloblastoma in vivo may be less significant than expected because of the extreme potency of immunotoxins observed in central nervous system tumors.
Contribution of Base Excision Repair, Nucleotide Excision Repair, and DNA Recombination to Alkylation Resistance of the Fission Yeast Schizosaccharomyces pombe (2000)

DNA damage is unavoidable, and organisms across the evolutionary spectrum possess DNA repair pathways that are critical for cell viability and genomic stability. To understand the role of base excision repair (BER) in protecting eukaryotic cells against alkylating agents, we generated Schizosaccharomyces pombe strains mutant for the mag1 3-methyladenine DNA glycosylase gene. We report that S. pombe mag1 mutants have only a slightly increased sensitivity to methylation damage, suggesting that Mag1-initiated BER plays a surprisingly minor role in alkylation resistance in this organism. We go on to show that other DNA repair pathways play a larger role than BER in alkylation resistance. Mutations in genes involved in nucleotide excision repair (rad13) and recombinational repair (rhp51) are much more alkylation sensitive than mag1 mutants. In addition, S. pombe mutant for the flap endonuclease rad2 gene, whose precise function in DNA repair is unclear, were also more alkylation sensitive than mag1 mutants. Further, mag1 and rad13 interact synergistically for alkylation resistance, and mag1 and rhp51 display a surprisingly complex genetic interaction. A model for the role of BER in the generation of alkylation-induced DNA strand breaks in S. pombe is discussed.
Acquired cardiovascular disease in adult patients with congenital heart disease (2017)

The adult congenital heart disease (ACHD) population is expanding and ageing. As survival rates of these patients continue to improve, acquired cardiac conditions, such as ischaemic cerebrovascular events and coronary artery disease (CAD), are likely to gain importance.1 However, limited information about the spectrum and the mechanisms of these conditions is available.

With regard to stroke, patients with ACHD are at a low absolute, yet a relative high risk compared with the general population. A retrospective analysis of 29 638 patients from Quebec showed that 1 in 11 men and 1 in 15 women between 18 and 64 years of age had a history of a stroke.2 In Euro Heart Survey on ACHD, in which the inclusion of patients with more complex lesions was quite high, 4% of 4168 patients experienced a cerebrovascular accident or transient ischaemic attack (TIA).3 In the Swedish Patient Registry of congenital heart disease, 0.5% of patients experienced an ischaemic stroke.4 A very low incidence was also found by Bokma et al , who reported that 56 out of 6904 patients had a history of ischaemic stroke/TIA.5 However, it should be taken into account that although population-based studies are of high importance, there are limitations inherited with this type of studies, as some details in the medical records cannot be adequately accessed. Jensen et al showed that in a cohort of 98 patients with cyanotic ACHD, findings consistent with previous stroke diagnosed with a brain MRI were reported in 47% of patients, while only 21% had a known medical history of a stroke, indicating the significant under-reporting and the high prevalence of ‘silent’ cerebrovascular events …
Enrichr: interactive and collaborative HTML5 gene list enrichment analysis tool (2013)

Enrichr is an easy to use intuitive enrichment analysis web-based tool providing various types of visualization summaries of collective functions of gene lists. Enrichr is open source and freely available online at: http://amp.pharm.mssm.edu/Enrichr.
Synthesis and characterization of semi‐interpenetrating polymer networks based on polyurethane and N‐isopropylacrylamide for wound dressing (2008)

Abstract Thermosensitive semi‐interpenetrating polymer networks (semi‐IPNs) composed of crosslinked poly( N ‐isopropylacrylamide) (PNiPAAm) and linear segmented polyurethane urea (SPUU) were synthesized via thermal initiated free radical polymerization. Synthesized semi‐IPNs of various compositions were characterized by Fourier transform infrared spectroscopy, water equilibrium swelling at different temperatures, drug lading, drug release, cell adhesion, and detachment. The semi‐IPN films of all the compositions were transparent in dry state and negative thermosensitivity in their swelling ratio, that is, lower swelling levels with increasing temperature. The drug release study revealed that the rate of drug release is fast in case of pure SPUU compared to PNiPAAm and semi‐IPN film. Drug release depended mainly on solubility of the drugs and physical networks between SPUU and PNiPAAm. Finally NIH3T3 cells were seeded on the semi‐IPN films and found that cells were securely attached and proliferated to confluence. Upon cooling, cells were detached from the semi‐IPN films. Therefore, the semi‐IPN films may be good candidate materials for wound dressing applications. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Effects of intense pulsed light on sun‐damaged human skin, routine, and ultrastructural analysis* (2002)

Under these conditions, IPL induces minimal morphologic changes in mildly sun-damaged skin. Some esthetic improvement may be secondary to clearing of Demodex organisms and reduction of associated lymphocytic infiltrate.
Taurolidine-citrate lock solution (TauroLock) significantly reduces CVAD-associated grampositive infections in pediatric cancer patients (2008)

The use of Taurolidin/Citrate (TauroLock) significantly reduced the number and incidence density of primary catheter-associated BSI due to CoNS and MRSE in pediatric cancer patients.
Structure-Switching Aptamer Triggering Hybridization Chain Reaction on the Cell Surface for Activatable Theranostics (2015)

The ability to probe low-abundance biomolecules or transport a high-load drug in target cells is essential for biology and theranostics. We develop a novel activatable theranostic approach by using a structure-switching aptamer triggered hybridization chain reaction (HCR) on the cell surface, which for the first time creates an aptamer platform enabling real-time activation and amplification for fluorescence imaging and targeting therapy. The aptamer probe is designed not to initiate HCR in its free state but trigger HCR on binding to the target cell via structure switching. The HCR not only amplifies fluorescence signals from a fluorescence-quenched probe for activatable tumor imaging but also accumulates high-load prodrugs from a drug-labeled probe and induces its uptake and conversion into cisplatin in cells for selective tumor therapy. An in vitro assay shows that this approach affords efficient signal amplification for fluorescence detection of target protein tyrosine kinase-7 (PTK7) with a detection limit of 1 pM. Live cell studies reveal that it provides high-contrast fluorescence imaging and highly sensitive detection of tumor cells, while renders high-efficiency drug delivery into tumor cells via an endocytosis pathway. The results imply the potential of the developed approach as a promising platform for early stage diagnosis and precise therapy of tumors.
Biochemical and physical properties of the prion protein from two strains of the transmissible mink encephalopathy agent (1992)

Transmissible mink encephalopathy (TME) has been transmitted to Syrian golden hamsters, and two strains of the causative agent, HYPER (HY) and DROWSY (DY), have been identified that have different biological properties. During scrapie, a TME-like disease, an endogenous cellular protein, the prion protein (PrPC), is modified (to PrPSc) and accumulates in the brain. PrPSc is partially resistant to proteases and is claimed to be an essential component of the infectious agent. Purification and analysis of PrP from hamsters infected with the HY and DY TME agent strains revealed differences in properties of PrPTME sedimentation in N-lauroylsarcosine, sensitivity to digestion with proteinase K, and migration in polyacrylamide gels. PrPC and HY PrPTME can be distinguished on the basis of their relative solubilities in detergent and protease sensitivities. PrPTME from DY-infected brain tissue shared solubility characteristics of PrP from both uninfected and HY-infected tissue. Limited protease digestion of PrPTME revealed strain-specific migration patterns upon polyacrylamide gel electrophoresis. Prolonged proteinase K treatment or N-linked deglycosylation of PrPTME did not eliminate such differences but demonstrated the PrPTME from DY-infected brain was more sensitive to protease digestion than HY PrPTME. Antigenic mapping of PrPTME with antibodies raised against synthetic peptides revealed strain-specific differences in immunoreactivity in a region of the amino-terminal end of PrPTME containing amino acid residues 89 to 103. These findings indicate that PrPTME from the two agent strains, although originating from the same host, differ in composition, conformation, or both. We conclude that PrPTME from the HY and DY strains undergo different posttranslational modifications that could explain differences in the biochemical properties of PrPTME from the two sources. Whether these strain-specific posttranslational events are directly responsible for the distinct biological properties of the HY and DY agent strains remains to be determined.
Tangible bits (1997)

Article Free Access Share on Tangible bits: towards seamless interfaces between people, bits and atoms Authors: Hiroshi Ishii MIT Media Laboratory, Tangible Media Group, 20 Ames Street, Cambridge, MA MIT Media Laboratory, Tangible Media Group, 20 Ames Street, Cambridge, MAView Profile , Brygg Ullmer MIT Media Laboratory, Tangible Media Group, 20 Ames Street, Cambridge, MA MIT Media Laboratory, Tangible Media Group, 20 Ames Street, Cambridge, MAView Profile Authors Info & Claims CHI '97: Proceedings of the ACM SIGCHI Conference on Human factors in computing systemsMarch 1997 Pages 234–241https://doi.org/10.1145/258549.258715Published:27 March 1997Publication History 2,110citation21,884DownloadsMetricsTotal Citations2,110Total Downloads21,884Last 12 Months2,302Last 6 weeks275 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Oscillatory Compared with Continuous High Pressure Sterilization on Bacillus stearothermophilus Spores (1994)

ABSTRACT TO ESTABLISH a sterilization method with minimal heating, the effect of high pressure on bacteriostasis was studied using thermoduric spores of Bacillus stearothermophilus . After exposure to 800 MPa for 60 min at 60°C, the spore count decreased from 10 6 to 10 2 /mL. However, exposure to the same pressure at room temperature did not cause significant change in spore numbers. The synergistic effect of high pressurization on the bacteriostatic action of sucrose fatty acid ester at low concentration (&lt; 10 ppm) was pronounced with sucrose palmitic acid ester but not with sucrose stearic acid ester. Oscillatory pressurization was more effective for spore sterilization. Six cycles oscillation of 5‐min pressurization with 400 MPa at 70°C decreased the spore count from 10 6 to 10 2 /mL, and with 600 MPa, complete sterilization was achieved.
Effect of nasal CPAP treatment on plasma volume, aldosterone and 24‐h blood pressure in obstructive sleep apnoea (1996)

Polycythaemia, peripheral oedema formation and hypertension have classically been described in association with obstructive sleep apnoea (OSA). However, there is very limited information about blood volume in OSA and how it changes during long-term treatment with nasal continuous positive airway pressure (nCPAP). Plasma (PV) and red-cell volumes (RCV), 24-h ambulatory blood pressure (BP), 24-h natriuresis and morning plasma aldosterone, renin activity and atrial natriuretic peptide in 11 men with a mean age of 47 y (range 37-55), apnoea index (AI) of 55 (22-106), body mass index of 36 (30-43) and seated BP of > or = 140/90 mmHg without any medication were measured. BP-measurements were repeated after 3 weeks and all measurements after 3 mo of nCPAP treatment. Aldosterone and 24-h mean heart rates decreased during treatment. Twenty-four-h BP decreased after 3 weeks but that decrease did not persist after 3 mo of treatment. There was a relationship between changes in night-time mean BP and PV and aldosterone. The haematocrit declined in every patient. No significant changes were found in the mean PV or RCV. They were in all instances lower than has earlier been described for normal, non-obese subjects. These data also suggest that OSA causes divergent individual disturbances in blood volume homeostasis which can be corrected by nCPAP.
Chemical Fluid Deposition:  Reactive Deposition of Platinum Metal from Carbon Dioxide Solution (1999)

Reduction of dimethyl(cyclooctadiene)platinum(II) (CODPtMe2) with hydrogen in supercritical carbon dioxide solution at 80 °C yields continuous metal films on solid inorganic and polymer substrates and discreet metal clusters within 200 nm pores of an aluminum oxide membrane. X-ray photoelectron spectroscopy indicates the deposited films are free of ligand-derived contamination and are of comparable purity to those deposited by chemical vapor deposition (CVD) using the identical precursor at 250 °C. The solubility of CODPtMe2 and its ligand-derived reduction products (cyclooctane and methane) in CO2 at the deposition conditions obviates precursor volatility requirements associated with CVD and enhances purity via facilitated desorption of the organic byproducts from the nascent metal surface. The discovery that the reduction of organometallic compounds proceeds cleanly in CO2 is the basis for chemical fluid deposition (CFD), a new, generally useful approach to the low-temperature metallization of polymers and inorganic solids.