gebhart/patrepeval huggingface dataset:
Human CSF-1: Molecular Cloning and Expression of 4-kb cDNA Encoding the Human Urinary Protein (1987)

A 4-kilobase complementary DNA (cDNA) encoding human macrophage-specific colony-stimulating factor (CSF-1) was isolated. When introduced into mammalian cells, this cDNA directs the expression of CSF-1 that is structurally and functionally indistinguishable from the natural human urinary CSF-1. Direct structural analysis of both the recombinant CSF-1 and the purified human urinary protein revealed that these species contain a sequence of at least 40 amino acids at their carboxyl termini which are not found in the coding region of a 1.6-kilobase CSF-1 cDNA that was previously described. These results demonstrate that the human CSF-1 gene can be expressed to yield at least two different messenger RNA species that encode distinct but related forms of CSF-1.
Ultra-Sensitive Colorimetric Assay System Based on the Hybridization Chain Reaction-Triggered Enzyme Cascade Amplification (2016)

A versatile and ultrasensitive colorimetric detection platform has been developed based on the hybridization chain reaction (HCR)-triggered enzyme cascade amplification in this work. The proposal involves the preparation of two different hairpin DNA strands consisting of the H<sub>1</sub>, modified with glucose oxidase (GOx-H<sub>1</sub>) and H<sub>2</sub>, modified with horseradish peroxidase (HRP-H<sub>2</sub>). The H<sub>1</sub> and H<sub>2</sub> were composed of complementary sequence of nucleic acid target (T) and interlaced complementary stem-loop sequences. In the nucleic acid detection, the hybridization of T and its complementary sequence induces the autonomous assembly of GOx-H<sub>1</sub> and HRP-H<sub>2</sub> through the predictable HCR, accompanied by the formation of GOx/HRP enzyme pairs with a multiple enzymatic cascade. In contrast to the crude mixture of free GOx-H<sub>1</sub> and HRP-H<sub>2</sub>, the catalytic performance of enzyme cascade reaction has been significantly enhanced, which can be determined by monitoring the absorbance change of 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS<sup>2-</sup>), a typical substrate with hydrogen peroxide for the HRP. Furthermore, this platform can be utilized in the assay of biological substances by the introduction of corresponding aptamer (Apt), complementary strands (Com), and an assistant hairpin DNA strand (H<sub>Assist</sub>). In view of the signal amplification of HCR and the enhanced catalytic performance of cascaded enzymes, our colorimetric assay system exhibits excellent sensitivity, and the detection limits have been calculated to be 5.2 fM and 0.8 pM for the nucleic acid target (T as a model) and biological substances (ATP as a model), respectively.
Expression of cyclooxygenase-2 in malignant and benign breast tumors. (2003)

Cyclooxygenase-2 (COX-2) is reported to play an important role in carcinogenesis. We examined COX-2 expression in patients with breast cancer and benign breast tumors. Immunohistochemical staining revealed a high level of COX-2 expression in malignant lesions of invasive ductal carcinoma (IDC) at a rate of 40% and a low level of expression in 15% of adjacent normal-appearing breast epithelia. Similarly, in ductal carcinoma in situ (DCIS), a high level of COX-2 expression was found in 80% of malignant lesions and a low level of expression in 50% of normal epithelia. Reverse transcriptional polymerase chain reaction (RT-PCR) performed in 7 of these cases disclosed that COX-2 expression was restricted to the malignant lesion. Further, all 10 cases of fibroadenoma and 10 cases of intraductal papilloma, both of which are benign tumors, had a high level of COX-2 expression. When overexpression of COX-2 was analyzed in relation to the clinicopathological features of the patients, no characteristic correlation was noted. Our results demonstrated that COX-2 is expressed in mammary tissue during tumorigenesis of the breast gland, suggesting that the cyclooxygenase isoenzyme may be a target for the prevention and treatment of breast cancer.
Improving alignment of faces for recognition (2011)

Face recognition systems for uncontrolled environments often work through an alignment, feature extraction, and recognition pipeline. Effective alignment of faces is thus crucial as can be an entry point in the process and poor alignments can greatly affect recognition performance. The task of alignment is particularly difficult when a face comes from highly unconstrained environments or so called faces in the wild. A lot of recent research activity has focused on faces in the wild and even simple similarity or affine transformations have proven both effective and essential to achieving state of the art performance. In this paper we explore a straightforward, fast and effective approach to aligning faces based on detecting facial landmarks using Haar-like image features and a cascade of boosted classifiers. Our approach is reminiscent of widely used face detection approaches, but focused on much more detailed features of a face such eye centres, the nose tip and corners of the mouth. This process generates multiple candidates for each landmark and we present a fast and effective filtering strategy allowing us to find sets of landmarks that are consistent. Our experiments show that this approach can outperform contemporary methods and easily fits into popular processing pipelines for faces in the wild.
Results of Splenectomy for Idiopathic Thrombocytopenic Purpura (1993)

Seventy-two patients underwent splenectomy for idiopathic thrombocytopenic purpura between 1979 and 1990. Mean age at splenectomy was 36.4 years (range 11-73). Indications for splenectomy were corticodependence in 21 cases and resistance to steroids in 44 cases. Thirty-five patients had platelet kinetic studies by 51Cr alloplatelets; 22 of them had splenic sequestration. Hematologic results were evaluated on discharge, at 3 months and in the long term (median follow-up 5.4 years). We had no mortality, morbidity was seen in 7% of the cases. None of the patients suffered from secondary infectious complications. 89% had good results on discharge (> 120 x 10(9)/l), 72.6% at 3 months and 90% on long-term follow-up. Factors associated with good response to splenectomy included a high postoperative platelet count (more than 120 x 10(9)/l on discharge), younger age at the time of surgery, preoperative corticodependence and predominantly splenic sequestration.
Online sensing of palladium in flowing streams (2016)

Rapid palladium (Pd) catalyzed deallylation of an uncoloured reagent within a flowing stream affords a dose dependent colour formation that can be used for convenient online analysis of trace levels of Pd contamination using a modified HPLC instrument. An application to the online sensing of Pd breakthrough from a flow through Pd adsorption cartridge is described. An alternative configuration of the instrumentation allows the rapid (<1 min) and accurate measurement of Pd levels within samples injected via a conventional HPLC autosampler.
Extracellular matrix analogs as carriers for growth factors: In vitro fibroblast behavior (1993)

Repair of connective tissue involves interactions between growth factors (GFs) and extracellular matrix (ECM) molecules. On the other hand, biological biomaterials could be used to carry and deliver GFs to stimulate wound healing. In the present study, fibroblasts were cultivated in a serum-free culture medium onto collagen (type I), hyaluronic acid, chondroitin sulfate, fibronectin, or fibrin. FGF, EGF, or PDGF was incorporated within those substrates. Using immuno- and radiolabeling assays, the distribution of GFs within the ECM analogs was relatively uniform and GFs retained in substrates were dependent on the substrates. Fibroblast replication was determined by the incorporation of [3H]-thymidine and compared to control groups. On collagen or chondroitin sulfate, the incorporation of GFs did not significantly improve cell proliferation. On hyaluronic acid, the incorporation of FGF and PDGF enhanced cell replication at 48 h. The incorporation of PDGF in fibronectin enhanced cell replication. On fibrin, the incorporation of PDGF, EGF, and FGF significantly enhanced cell replication. However, cell replication on FGF-incorporated fibrin was higher by 48 h than that on fibrin in the presence of FGF-supplemented culture medium. Fibrin sustains the biological activity of GFs, FGF in particular, and can be a carrier for GF that stimulates cell replication.
Using Block Copolymer Micellar Thin Films as Templates for the Production of Catalysts for Carbon Nanotube Growth (2004)

We report a novel approach that uses block copolymer micelles as a means to create large area arrays of iron-containing nanoclusters capable of catalyzing the growth of carbon nanotubes (CNTs). The amphiphilic block copolymer poly(styrene-block-acrylic acid) (PS-b-PAA) forms micelles in solution which are capable of self-organizing into ordered structures on surfaces. By spin-coating these solutions onto a variety of substrates, we can create quasi-hexagonal arrays of PAA spheres within a PS matrix. The carboxylic acids groups in the PAA domains can be utilized in an ion-exchange protocol to selectively sequester iron ions, which results in iron-containing nanoclusters that are nearly monodisperse in size (diameter ∼8 nm) and patterned at a density of approximately 1011 particles per cm2. In principle, this route for synthesizing iron-containing nanoclusters offers the capability of controlling the cluster size and spacing by altering the molecular weight of the block copolymer. In this report, we demonstrate the ability of these block-copolymer-templated iron-containing nanocluster arrays to catalyze the growth of CNTs in a thermal chemical vapor deposition (CVD) process. We present transmission electron microscope (TEM) and scanning electron microscope (SEM) images of the as-grown CNTs still attached to their growth substrate, which allows us to characterize both the CNTs and the catalytic nanoclusters after CVD growth.
Architecting Waferscale Processors - A GPU Case Study (2019)

Increasing communication overheads are already threatening computer system scaling. One approach to dramatically reduce communication overheads is waferscale processing. However, waferscale processors [1], [2], [3] have been historically deemed impractical due to yield issues [1], [4] inherent to conventional integration technology. Emerging integration technologies such as Silicon-Interconnection Fabric (Si-IF) [5], [6], [7], where pre-manufactured dies are directly bonded on to a silicon wafer, may enable one to build a waferscale system without the corresponding yield issues. As such, waferscalar architectures need to be revisited. In this paper, we study if it is feasible and useful to build today's architectures at waferscale. Using a waferscale GPU as a case study, we show that while a 300 mm wafer can house about 100 GPU modules (GPM), only a much scaled down GPU architecture with about 40 GPMs can be built when physical concerns are considered. We also study the performance and energy implications of waferscale architectures. We show that waferscale GPUs can provide significant performance and energy efficiency advantages (up to 18.9x speedup and 143x EDP benefit compared against equivalent MCM-GPU based implementation on PCB) without any change in the programming model. We also develop thread scheduling and data placement policies for waferscale GPU architectures. Our policies outperform state-of-art scheduling and data placement policies by up to 2.88x (average 1.4x) and 1.62x (average 1.11x) for 24 GPM and 40 GPM cases respectively. Finally, we build the first Si-IF prototype with interconnected dies. We observe 100% of the inter-die interconnects to be successfully connected in our prototype. Coupled with the high yield reported previously for bonding of dies on Si-IF, this demonstrates the technological readiness for building a waferscale GPU architecture.
Ring Opening of Epoxides with Sodium Azide in Water. A Regioselective pH-Controlled Reaction (1999)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTRing Opening of Epoxides with Sodium Azide in Water. A Regioselective pH-Controlled ReactionFrancesco Fringuelli, Oriana Piermatti, Ferdinando Pizzo, and Luigi VaccaroView Author Information Dipartimento di Chimica, Università degli Studi di Perugia, via Elce di Sotto 8, 06123 Perugia, Italy Cite this: J. Org. Chem. 1999, 64, 16, 6094–6096Publication Date (Web):July 14, 1999Publication History Received2 March 1999Published online14 July 1999Published inissue 1 August 1999https://pubs.acs.org/doi/10.1021/jo990368ihttps://doi.org/10.1021/jo990368ibrief-reportACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views5645Altmetric-Citations100LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Azides,Ethers,Ions,Organic reactions,Selectivity Get e-Alerts
Supercritical Fluid in Polymer Science and Technology. I. Super-Rapid Enzymatic Hydrolysis of Cellulose with Supercritical Water Solubilization Pretreatment. (2001)

セルロースの超臨界水処理実験を, 流通式反応装置を用いて温度360-400℃, 圧力25-33MPa, 処理時間0.1-0.4秒で行った. 温度380℃, 圧力30MPaの超臨界水中では, きわめて短時間 (0.18-0.28秒) の処理でセルロースを高重合度セルロースおよびセロオリゴ糖・単糖へ変換できた. 可溶化セルロースおよびその再結晶化物 (セルロースII型結晶) に対する酵素活性を調べた結果, 未処理セルロースと比較して, 可溶化セルロースでは約13倍, 再結晶化物でも約4倍の酵素活性を示すことを見いだした. 本手法により, 可溶化したセルロースおよびセロオリゴ糖を高効率, かつ短時間の酵素反応 (0.5-24時間) でグルコースおよびセロビオースへ変換しえることを見いだした.
1-millimeter wave interferometer for the measurement of line integral electron density on TFTR (1985)

A two-pass interferometer at 285 GHz has been developed to measure the line-integrated electron density on the horizontal midplane of the toroidal fusion test reactor (TFTR). Presently, the interferometer employs a 2-mW solid-state source to supply the launch wave and a 2-mm klystron oscillator and, a harmonic mixer to provide a superheterodyne front end. The transmission system consists of 25 m of C-band rectangular waveguide, adjustable miter bends, and a spherical mirror in the vacuum vessel with a total round trip transmission loss of 21 dB. The interferometer signal to noise is ≳50 dB. Utilization of a feed forward tracking system provides long-term stable operation. The interferometer routinely provides real-time feedback control for the gas injection system and a permissive for neutral beam operation.
Factor VIII-Fc Fusion Protein Shows Extended Half-Life and Hemostatic Activity in Hemophilia A Dogs. (2009)

Abstract Abstract 545 A recombinant B-domain-deleted factor VIII-Fc (rFVIIIFc) fusion protein was created as an approach to extend the half-life of FVIII. The pharmacokinetics and pharmacodynamics of rFVIIIFc were evaluated in the Chapel Hill colony of hemophilia A dogs. These dogs have a severe hemophilic phenotype comparable to the severe form of human disease with F.VIII &lt; 1%. A single intravenous dose (125 IU/kg) was administered to four dogs and immediately corrected the clotting to normal as measured by whole blood clotting time (WBCT) and aPTT. The WBCT remained below 20 min, the time consistent with FVIII:C &gt; 1%, through approximately 96 h. The range of WBCT in our normal dogs is 8 to 12 min. The concentration of rFVIIIFc in the plasma was measured by ELISA and the terminal half-life was 15.7 ± 1.7 hr. Similar results were obtained when rFVIIIFc was measured using a FVIII-specific chromogenic activity assay (half-life was 15.4 ± 0.3 hr). The concentration vs. time curves were similar using both methods. The activity data were converted to ng/mL using the specific activity of the test article that was used to dose the animals, and these data correlated well with the ELISA data, thus demonstrating that the protein that was measured by ELISA was fully active. Two of the dogs also received a single dose of recombinant B-domain deleted FVIII (rBDD-FVIII, ReFacto®), 114 IU/kg for one dog and 120 IU/kg rBDD-FVIII for the other, and then received rFVIIIFc (125 IU/kg) 72 hr later in a cross over design. Clotting was corrected to normal immediately after dosing with both rBDD-FVIII and rFVIIIFc (determined by WBCT and clotting activity measured using an aPTT assay). However, the WBCT normalization after rFVIIIFc lasted for approximately twice as long compared to rBDD-FVIII and the half-lives determined from the ELISA data for FVIIIFc (15.7 ± 1.7 hr) were twice those determined for rBDD-FVIII (7.0 hr and 6.7 hr). No adverse clinical signs were detected with any of the infusions. Therefore construction of an Fc fusion of FVIII produces a molecule with a defined mechanism of action that has an increased half life and the potential to provide prolonged protection from bleeding. Disclosures: Dumont: Biogen Idec (Syntonix Subsidiary): Employment. Kamphaus:Biogen Idec (Syntonix Subsidiary): Employment. Fraley:Biogen Idec/Syntonix Subsidiary: Employment. Ashworth:Biogen Idec (Syntonix Subsidiary): Employment. Bitonti:Biogen Idec/Syntonix Subsidiary: Employment.
Shadowing Dynamic Scenes with Arbitrary BRDFs (2009)

Abstract We present a real‐time relighting and shadowing method for dynamic scenes with varying lighting, view and BRDFs. Our approach is based on a compact representation of reflectance data that allows for changing the BRDF at run‐time and a data‐driven method for accurately synthesizing self‐shadows on articulated and deformable geometries. Unlike previous self‐shadowing approaches, we do not rely on local blocking heuristics. We do not fit a model to the BRDF‐weighted visibility, but rather only to the visibility that changes during animation. In this manner, our model is more compact than previous techniques and requires less computation both during fitting and at run‐time. Our reflectance product operators can re‐integrate arbitrary low‐frequency view‐dependent BRDF effects on‐the‐fly and are compatible with all previous dynamic visibility generation techniques as well as our own data‐driven visibility model. We apply our reflectance product operators to three different visibility generation models, and our data‐driven model can achieve framerates well over 300Hz.
Nonlinear thermal distortion in YAG rod amplifiers (1998)

Yttrium aluminum garnet (YAG) has thermal, mechanical, and optical properties that vary strongly with temperature. In this paper, we show that when such properties are taken into account the resulting thermally induced distortions in rod amplifiers cannot be described as quadratically varying with rod radius, and thus cannot be completely corrected using a simple spherical lens. In addition, we show that for operation with a coolant temperature at 77 K, thermally induced distortion is minimal and birefringence is substantially reduced when compared to that during operation with a coolant at room temperature.
[Determination of DNA-binding proteins by countercurrent isotachopheresis on nitrocellulose membranes. I. Antibodies to DNA and its adducts]. (1995)

A very strong electroosmotic counterflow was produced on nitrocellulose membranes during isotachophoresis in a system of 0.06 M Tris-HCl (pH 6.7) as the leading electrolyte and 0.012 M Tris-beta-alanine (pII 8.6) as the terminating one. This counterflow was equal in rate and opposite in direction to the migration of the Cl-/beta-alanine boundary. The rate of counterflow was much higher than the rate of migration of any organic anions, including different proteins. Double-stranded and single-stranded DNA or its adducts were fixed on the nitrocellulose membrane, and the membrane was blocked with unrelated proteins. DNA-binding proteins, namely antibodies to DNA, followed by peroxidase-conjugated anti-IgG, were introduced into the counterflow, which transferred them one after another to the DNA spots. Thus, sequential binding and washing was performed automatically. In this way, antibodies were detected to ds- and ss-DNA, to BrdU-DNA, to Z-DNA, to biotinylated DNA and DNA modified with trans-Pt, as well as development of biotinylated DNA dots by steptavidin-peroxidase.
Visualization of individual carbon nanotubes with fluorescence microscopy using conventional fluorophores (2003)

We demonstrate that individual carbon nanotubes (CNTs) can be visualized with fluorescence microscopy through noncovalent labeling with conventional fluorophores. Reversal of contrast in fluorescence imaging of the CNTs was observed when performing labeling procedure in a nonpolar solvent. Our results are consistent with a CNT-fluorophore affinity mediated by hydrophobic interaction. The reverse-contrast images also provide clear indication of nanotube location.
Biosynthesis and Mode of Action of Lantibiotics (2005)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBiosynthesis and Mode of Action of LantibioticsChampak Chatterjee, Moushumi Paul, Lili Xie, and Wilfred A. van der DonkView Author Information Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois Cite this: Chem. Rev. 2005, 105, 2, 633–684Publication Date (Web):February 9, 2005Publication History Received13 July 2004Published online9 February 2005Published inissue 1 February 2005https://pubs.acs.org/doi/10.1021/cr030105vhttps://doi.org/10.1021/cr030105vresearch-articleACS PublicationsCopyright © 2005 American Chemical SocietyRequest reuse permissionsArticle Views7267Altmetric-Citations631LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Chemical structure,Genetics,Lipids,Monomers,Peptides and proteins Get e-Alerts
Effects of chronic heart failure on the responsiveness to angiotensin I and to angiotensin converting enzyme inhibition with cilazapril in rats. (1989)

1. The effects of heart failure due to chronic myocardial infarction on the responsiveness to injected angiotensin I and ACE inhibition by intravenous cilazapril (1 mg kg-1) were evaluated. 2. For this purpose one group of 17 rats with a 4-week old myocardial infarction was compared with a group of 10 sham operated rats. 3. Heart failure increased markedly the responsiveness of the renal and mesenteric vascular beds to ACE inhibition which produced a vasodilation in these two vascular beds. 4. This increased responsiveness was most likely due to a stimulation of the renin-angiotensin system without any change of sensitivity to angiotensin I of the renal and mesenteric vascular beds. 5. Cilazapril produced the same level of ACE inhibition in both groups of rats.