gebhart/patrepeval huggingface dataset:
Long-Term Erythropoietin Expression in Rodents and Non-Human Primates Following Intramuscular Injection of a Replication-Defective Adenoviral Vector (1997)

Erythropoietin (Epo)-responsive anemia is a debilitating complication of chronic renal failure and human immunodeficiency virus (HIV) infection that effects more than 150,000 Americans. Patients with Epo-responsive anemias are currently treated with repeated injections of recombinant human Epo. In the studies described in this report, we have examined the safety and efficacy of using a single intramuscular (i.m.) injection of replication-defective adenoviral vectors (RDAd) encoding Epo for the treatment of Epo-responsive anemias in both mice and non-human primates. Our results demonstrate that there is a threshold dose of virus (2.5-8 x 10(7) pfu/gram of body weight) which is required to obtain long-term Epo expression and polycythemia in both species. A single i.m. injection of mice with 10(9) pfu of an RDAd encoding murine Epo (AdmEpo) resulted in elevations in hematocrits from control values of 49 +/- 0.9% to treated values of 81 +/- 3%, which were stable for more than 1 year. Similarly, a single i.m. injection of a monkey with 4 x 10(11) pfu of an RDAd-encoding simian Epo (AdsEpo) resulted in elevations of hematocrits from control levels of 40% to treated levels of > or =70%, which were stable for 84 days. Intramuscular injection of monkeys with AdsEpo appeared to be safe in that we did not detect abnormalities in chest X-rays, serum chemistries, hematologic, or clotting profiles (apart from elevated hematocrits) or organ histologies during the 84-day time course of the experiment. Taken together, these results suggest the feasibility of using i.m. injection of RDAd for the treatment of Epo-responsive anemias in humans.
Acyclovir prophylaxis for herpes simplex virus infection (1987)

ACV is an effective agent for the treatment and prophylaxis of HSV infections in both IC and immunologically normal individuals. The drug is well tolerated in both populations and is not significantly associated with clinical or laboratory toxicities. Because of the great potential benefit and low risk, organ transplant recipients and patients with hematologic malignancies undergoing induction chemotherapy should be screened routinely for HSV antibodies; seropositive individuals should receive prophylactic ACV during the period of most profound immunosuppression. Immunologically normal individuals with frequently recurring genital HSV or serious complications associated with outbreaks are candidates for long-term suppression with ACV.
Antimicrobial, Antioxidant, and Cytotoxic Activities of the Essential Oil of Tarchonanthus camphoratus (2013)

The leaf essential oil of Tarchonanthuscamphoratus(As teraceae) was obtained by hydrodistillation and analyzed by GC-MS. Fifty-six components were characterized, representing 94.2% of the total oil with oxygenated monoterpenes (48.3%) and oxygenated sesquiterpenes (32.7%) as the major groups. The principal constituents were identified as endo-fenchol (21.2%), trans-pinene hydrate (8.8%), caryophyllene oxide (7.5%), α-terpineol (6.4%), τ-cadinol (6.4%), and α-cadinol (5.2%). The essential oil was evaluated for its antimicrobial activity using a disc diffusion assay resulting in the moderate inhibition of a number of common human pathogenic bacteria, including methicillin-resistant Staphylococcus aureus(MRSA) and the yeast Candida albicans. The inhibition zones varied from 10 to 14mm/disc. Furthermore, the antioxidant capacity of the essential oil was examined using an in vitroradical scavenging activity test. The T. camphoratus essential oil scavenged 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), resulting in an IC 50 value of 5.6 mg/mL. At concentrations of 100 and 50μg/mL, the oil showed cytotoxic activity, with growth inhibition of 59.1% (±4.2), and 16.2% (±8.7) against HT29 tumor cells (human colonic adenocarcinoma cells), respectively(IC 50 = 84.7 ± 7.5 μg/mL).
Incremental learning with sample queries (1998)

The classical theory of pattern recognition assumes labeled examples appear according to unknown underlying class conditional probability distributions where the pattern classes are picked randomly in a passive manner according to their a priori probabilities. This paper presents experimental results for an incremental nearest-neighbor learning algorithm which actively selects samples from different pattern classes according to a querying rule as opposed to the a priori probabilities. The amount of improvement of this query-based approach over the passive batch approach depends on the complexity of the Bayes rule.
TRK-Fused Gene (TFG) Is a New Partner of ALK in Anaplastic Large Cell Lymphoma Producing Two Structurally DifferentTFG-ALK Translocations (1999)

Anaplastic large cell lymphoma (ALCL) is associated with the t(2;5)(p23;q35), which generates the NPM-ALK fusion gene encoding an 80-kD protein. Several studies have suggested that genes other than NPM may be fused to the ALK gene. Here we have identified TRK-fused gene (TFG) as a new ALK partner in 2 ALCL, 1 of which exhibited a t(2;3)(p23;q21). In these cases, TFG was involved in 2 different fusion genes, TFG-ALK(S) and TFG-ALK(L), coding respectively 85-kD and 97-kD chimeric proteins. The ALK breakpoint in these translocations was the same as in the classic t(2;5) translocation. These 2 proteins were both active in an in vitro tyrosine kinase assay showing that the new cloned cDNA sequences are translated into chimeric proteins with functional activity. These findings indicate that TFG can provide an alternative to NPM as a fusion partner responsible for activation of the ALK and the pathogenesis of ALCL.
Physician's cancer chemotherapy drug manual 2008 (2003)

Cobitis elongatoides is a small sized freshwater fish species that is widely distributed in Europe, especially in Croatia. In this study, the complete mitochondrial genome of C. elongatoides is sequenced to be 16,540 bp in length, including 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, a control region and the origin of the light strand replication. The overall base composition of C. elongatoides in descending order is A 29.37%, T 28.53%, C 25.34%, and G 16.76%, with a slight A + T bias. The mitogenome sequence data may provide useful information to the population genetics analysis of C. elongatoides and the elucidation of evolutionary mechanisms in Cobitidae.
Monomeric and dimeric CXCL12 inhibit metastasis through distinct CXCR4 interactions and signaling pathways (2011)

Chemokines and chemokine receptors are extensively and broadly involved in cancer metastasis. Previously, we demonstrated that epigenetic silencing of the chemokine CXCL12 sensitizes breast and colon cancer cells to endocrine signaling and metastasis to distant tissues. Yet, the precise mechanism whereby CXCL12 production by tumor cells regulates dissemination remains unclear. Here, we show that administration of CXCL12 extended survival of tumor-bearing mice by potently limiting metastasis of colorectal carcinoma or murine melanoma. Because secreted CXCL12 is a mixture of monomeric and dimeric species in equilibrium, oligomeric variants that either promote (monomer) or halt (dimer) chemotaxis were used to dissect the mechanisms interrupting carcinoma metastasis. Monomeric CXCL12 mobilized intracellular calcium, inhibited cAMP signaling, recruited β-arrestin-2, and stimulated filamentous-actin accumulation and cell migration. Dimeric CXCL12 activated G-protein-dependent calcium flux, adenylyl cyclase inhibition, and the rapid activation of ERK1/2, but only weakly, if at all, recruited arrestin, stimulated actin polymerization, or promoted chemotaxis. NMR analyses illustrated that CXCL12 monomers made specific contacts with CXCR4 that were lost following dimerization. Our results establish the potential for inhibiting CXCR4-mediated metastasis by administration of CXCL12. Chemokine-mediated migration and β-arrestin responses did not dictate the antitumor effect of CXCL12. We conclude that cellular migration is tightly regulated by selective CXCR4 signaling evoked by unique interactions with distinct ligand quaternary structures.
Globin Gene Silencing in Primary Erythroid Cultures (1997)

There are numerous similarities between the erythroid and megakaryocytic lineages which suggest that commitment to either lineage occurs relatively late in hematopoiesis. Commitment toward megakaryocyte development requires obligatory silencing of erythroid-specific genes. Therefore, we investigated the effects of interleukin-6, a known inducer of thrombocyte production, on globin gene expression during erythroid differentiation. Studies in K562 cells demonstrated inhibition of gamma globin gene mRNA production and chain biosynthesis in the presence of exogenous interleukin-6 which was abrogated by anti-interleukin-6 monoclonal antibody. Similar studies in primary erythroid progenitors showed inhibition of burst-forming unit-erythroid colony formation when interleukin-6 was added late in cultures with decreased gamma and beta globin gene mRNA production. Protein binding studies demonstrated an increase in activator protein-1 binding to its consensus sequence by 24 h of interleukin-6 treatment. Inhibition of activator protein-1 gene activity had no effect on gamma gene silencing by interleukin-6. A potential interleukin-6 response element was identified in the gamma globin gene. Interleukin-6 treatment led to a rapid increase in protein binding to the target DNA sequence. These results suggest that interleukin-6 may play an important role in globin gene silencing during megakaryocytic lineage commitment.
Gate-polarity-dependent doping effects of H 2 O adsorption on graphene/SiO 2 field-effect transistors (2020)

Abstract Due to its huge surface-to-volume ratio, graphene has become one of the most popular materials for sensors. However, H 2 O molecules in atmospheric environments can cause the instability of graphene devices, which greatly limits the practical applications of graphene devices. The charge transfer between graphene and adsorbed H 2 O molecules has been proved previously by first-principle studies, but experimental demonstrations are still lacking. Here, gate-polarity-dependent doping behaviors of adsorbed H 2 O molecules on a graphene/SiO 2 field-effect transistor (GFET) are experimentally investigated. The results indicate that the orientation of the adsorbed H 2 O can be affected by the gate-voltage polarity due to the dipolar interaction, which leads to the amphoteric doping behavior of adsorbed H 2 O molecules. With reducing the graphene layer number, the amphoteric doping behavior is more sensitive to the gate voltage polarity. Our results are helpful for constructing high performance graphene-based sensors and enhancing the stability of GFETs.
The Effect of Mannan Oligosaccharide Supplementation on Body Weight Gain and Fat Accrual in C57Bl/6J Mice (2009)

The prevalence of obesity in industrialized societies has become markedly elevated. In contrast, model organism research shows that reducing caloric intake below ad libitum levels provides many health and longevity benefits. Despite these benefits, few people are willing and able to reduce caloric intake over prolonged periods. Prior research suggests that mannooligosaccharide (MOS or mannan) supplementation can increase lifespan of some livestock and in rodents can reduce visceral fat without reducing caloric intake. Hence, we tested the effect of MOS supplementation as a possible calorie restriction (CR) mimetic (CRM) in mice. C57Bl/6J male mice were fed a high-fat "western" type diet with or without 1% MOS (by weight) supplementation (n = 24/group) from 8 to 20 weeks of age. Animals were housed individually and provided 95% of ad libitum food intake throughout the study. Body weight was measured weekly and body composition (lean and fat mass) measured noninvasively every 3 weeks. Individual fat depot weights were acquired by dissection at study completion. Supplementation of a high-fat diet with 1% MOS tended to reduce total food intake (mean +/- s.d.; control (CON): 293.69 +/- 10.53 g, MOS: 288.10 +/- 11.82 g; P = 0.09) during the study. Moreover, MOS supplementation had no significant effect on final body weight (CON: 25.21 +/- 2.31 g, MOS: 25.28 +/- 1.49 g; P = 0.91), total fat (CON: 4.72 +/- 0.90 g, MOS: 4.82 +/- 0.83 g; P = 0.69), or visceral fat (CON: 1.048 +/- 0.276 g, MOS: 1.004 +/- 0.247 g; P = 0.57). Contrary to previous research, MOS supplementation had no discernable effect on body weight gain or composition during this 12-week study, challenging the potential use of MOS as a CRM or body composition enhancer.
Reliable information service for Internet computer conferencing (2002)

The Conferencing Information Service (CIS) described is developed to provide reliable information to users about ongoing computer conferences throughout the Internet. CIS is composed of three major components: the information daemon (ID), to store and retrieve information about the ongoing conferences in the Internet; the conference announcer (CA), to broadcast conference information to the IDs; the user interface (UI), to retrieve the current conference information from the IDs, to display the retrieved information to the user in an X-window based interface, and to allow the users to start a new conference or join a selected ongoing conference.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Use of an Acellular Dermal Allograft for Dural Replacement: An Experimental Study (1999)

In this porcine model, an allograft of acellular dermis is a nearly ideal dural replacement. AlloDerm, the human equivalent of XenoDerm, would be an allograft of acellular dermis after implantation in human subjects. On the basis of this study and previous work with AlloDerm in other reconstructive applications, it is proposed that this material could be similarly used for duraplasty in human subjects.
Disialoganglioside GD2 anti‐idiotypic monoclonal antibodies (1993)

Disialoganglioside GD2 is widely expressed among neuroblastomas, melanomas, small-cell lung carcinoma, sarcomas and brain tumors. Immunity directed against this antigen may have anti-tumor utility. Since GD2 is poorly immunogenic, anti-idiotypic antibodies may serve as alternative tumor vaccines. Monoclonal antibody 3F8, a murine IgG3 specific for GD2, has shown excellent tumor-targeting ability in vitro and in vivo. LOU/CN rats were immunized with 3F8 and their spleens were used in somatic-cell hybridization, using SP2/0, P3 and Y3 as fusion partners. Six anti-idiotypic (anti-id) MAbs (C2D8, Idio-2, AIG4, C2H7, C4E4, A2A6) were selected based on their reactivity with 3F8 and non-reactivity with murine IgG3 myelomas. Specificity of each anti-id was demonstrated by using various ELISA: (i) lack of direct binding to solid phase myelomas and serum proteins; (ii) inability of other myelomas to inhibit anti-id binding to 3F8; (iii) absence of cross-reactivity of other myelomas to solid-phase anti-id; (iv) lack of inhibition by anti-id of binding of other ganglioside antibodies to their antigens. Antigen specificity was further examined by inhibition of binding of 3F8 to GD2 on immuno-thin-layer chromatography, and by inhibition of 3F8 immunostaining of neuroblastoma cell lines. These 6 antibodies were demonstrated to be distinct, in view of their cross-reactivity, fusion partners and relative strength of binding to 3F8. Anti-GD2 antibodies were induced after immunization with these anti-id antibodies in C57Bl/6 mice. These rat anti-3F8-idiotypic antibodies with exquisite specificity for anti-GD2 antibodies may be useful in vaccine construction.
Comparison among strains of porcine reproductive and respiratory syndrome virus for their ability to cause reproductive failure. (1996)

The effects of PRRSV on reproductive performance are strain dependent and this should be considered in making a tentative diagnosis on the basis of clinical observations.
TCR-Engineered T Cells Meet New Challenges to Treat Solid Tumors: Choice of Antigen, T Cell Fitness, and Sensitization of Tumor Milieu (2013)

Adoptive transfer of T cells gene-engineered with antigen-specific T cell receptors (TCRs) has proven its feasibility and therapeutic potential in the treatment of malignant tumors. To ensure further clinical development of TCR gene therapy, it is necessary to target immunogenic epitopes that are related to oncogenesis and selectively expressed by tumor tissue, and implement strategies that result in optimal T cell fitness. In addition, in particular for the treatment of solid tumors, it is equally necessary to include strategies that counteract the immune-suppressive nature of the tumor micro-environment. Here, we will provide an overview of the current status of TCR gene therapy, and redefine the following three challenges of improvement: "choice of target antigen"; "fitness of T cells"; and "sensitization of tumor milieu." We will categorize and discuss potential strategies to address each of these challenges, and argue that advancement of clinical TCR gene therapy critically depends on developments toward each of the three challenges.
Sequential Expression of OX2 and Thy-1 Glycoproteins on the Neuronal Surface during Development (1987)

The related neuronal surface glycoproteins, Thy-1 and 0X2, are shown here to have a reciprocal pattern of expression during development in rat cerebellum. 0X2 appears at axonogenesis, on neuronal cell bodies and their axons. Immunohistochemical staining for this molecule then gradually wanes, and over the same period the cells acquire their first, weak staining for Thy-1. Intense Thy-1 expression occurs during the period of dendritic growth.
Privacy and accountability for location-based aggregate statistics (2011)

A significant and growing class of location-based mobile applications aggregate position data from individual devices at a server and compute aggregate statistics over these position streams. Because these devices can be linked to the movement of individuals, there is significant danger that the aggregate computation will violate the location privacy of individuals. This paper develops and evaluates PrivStats, a system for computing aggregate statistics over location data that simultaneously achieves two properties: first, provable guarantees on location privacy even in the face of any side information about users known to the server, and second, privacy-preserving accountability (i.e., protection against abusive clients uploading large amounts of spurious data). PrivStats achieves these properties using a new protocol for uploading and aggregating data anonymously as well as an efficient zero-knowledge proof of knowledge protocol we developed from scratch for accountability. We implemented our system on Nexus One smartphones and commodity servers. Our experimental results demonstrate that PrivStats is a practical system: computing a common aggregate (e.g., count) over the data of 10,000 clients takes less than 0.46 s at the server and the protocol has modest latency (0.6 s) to upload data from a Nexus phone. We also validated our protocols on real driver traces from the CarTel project.
Inhibition of the replication of native and 3′‐azido‐2′,3′‐dideoxy‐thymidine (AZT)‐resistant simian immunodeficiency virus (SIV) by 3‐nitrosobenzamide (1993)

The 3-nitrosobenzamide (NOBA) drug abolishes SIV replication sharply at 20 microM concentration when CEM x 174 cells are preincubated for 1 h with the drug prior to viral infection. Treatment of CEM x 174 cells with 20 microM NOBA resulted in the inhibition of the synthesis of the DNA sequence coding for the gag gene, as determined by the PCR technique. Cell viability was directly proportional to the antiviral action of NOBA. Replication of AZT-resistant SIV 23740 in MMU 23740 cells in vitro was suppressed by NOBA in a concentration-dependent manner without significant effects on cell viability. Reverse transcriptase activity of SIVmac239 was unaffected by NOBA up to 800 microM concentration. Preincubation of two SIV strains with NOBA completely abolished their infectivity in human PHA-PBL cells. Replication of two strains of SIV in PHA-PBL cells was also inhibited by NOBA.
THE ORGANIZATION OF BEHAVIORAL REPERTOIRE IN MOTOR CORTEX (2006)

Motor cortex in the primate brain was once thought to contain a simple map of the body's muscles. Recent evidence suggests, however, that it operates at a radically more complex level, coordinating behaviorally useful actions. Specific subregions of motor cortex may emphasize different ethologically relevant categories of behavior, such as interactions between the hand and the mouth, reaching motions, or defensive maneuvers to protect the body surface from impending impact. Single neurons in motor cortex may contribute to these behaviors by means of their broad tuning to idiosyncratic, multijoint actions. The mapping from cortex to muscles is not fixed, as was once thought, but instead is fluid, changing continuously on the basis of feedback in a manner that could support the control of higher-order movement parameters. These findings suggest that the motor cortex participates directly in organizing and controlling the animal's behavioral repertoire.
Oxygen radicals from photoirradiated human hair: An ESR and fluorescence study (2000)

Exposure of hair to light causes degradation, the precise mechanism of which is not completely understood. Oxygen free radicals (superoxide and hydroxyl) are believed to be involved, and a variety of indirect evidence has supported this. However, to date, direct observation of oxyradicals from photoirradiated hair has been lacking. We have used complementary electron spin resonance and fluorescence t chniques to assess oxyradical production when human hair is irradiated under UVA and visible light 0 •--&amp;gt; 320 nm). ESR studies include direct observation of intrinsic melanin and keratin radicals as well as spin trapping with DMPO. The fluorescence t chnique is based on terephthalic acid dianion (TA) as a hydroxyl radical probe. Radical scavengers are used to compete with the probe (TA or DMPO). Our results indicate that (a) oxyradicals are produced uring photoirradiation, (b) terephthalate is a convenient method to study hydroxyl production in hair, (c) radical production upon irradiation varies with hair type, and (d) the effects of radical scavengers suggest potential implications for hair care product development.