gebhart/patrepeval huggingface dataset:
Regulation of Cell Migration by the C2 Domain of the Tumor Suppressor PTEN (2004)

PTEN is a tumor suppressor protein that dephosphorylates phosphatidylinositol 3,4,5 trisphosphate and antagonizes the phosphatidylinositol-3 kinase signaling pathway. We show here that PTEN can also inhibit cell migration through its C2 domain, independent of its lipid phosphatase activity. This activity depends on the protein phosphatase activity of PTEN and on dephosphorylation at a single residue, threonine(383). The ability of PTEN to control cell migration through its C2 domain is likely to be an important feature of its tumor suppressor activity.
Mutated bacteriorhodopsins-versatile media in optical image processing (1994)

Mutated bacteriorhodopsins are considered as versatile media in optical image processing. The following topics are discussed: the biological function of bacteriorhodopsin (BR); the photocycle of BR; light-controlled absorption of BR films; BR films in transmission- and reflection-type dynamic holograms; holographic interferometry (image processing in the time domain); holographic pattern recognition (image processing in the space domain).< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Integrated optics in silicon and SiGe-heterostructures (1996)

This paper reviews various techniques and ideas in the field of integrated optics in silicon, mainly focused on silicon in conjunction with germanium. We will discuss different approaches for waveguides, passive and active components in silicon as well as recent developments in the fabrication and performance of such components. For waveguides in silicon, the characteristics such as losses and spot-sizes are given, showing that silicon could be an attractive candidate for integrated optical devices.
Foot Depth Map Point Cloud Completion using Deep Learning with Residual Blocks (2017)

Fit is extremely important in footwear as fit largely determines performanceand comfort. Current footwear fit estimation mainly usesonly shoe size, which is extremely limited in characterizing theshape of a foot or the shape of a shoe. 3D scanning presents asolution to this, where a foot shape can be captured and virtuallyfit with shoe models. Traditional 3D scanning techniques have theirown complications however, stemming from their need to collectviews covering all aspects of an object. In this work we explore adeep learning technique to compete a foot scan point cloud frominformation contained in a single depth map view. We examine thebenefits of implementing residual blocks in architectures for this application,and find that they can improve accuracies while reducingmodel size and training time.
Probing Charged Impurities in Suspended Graphene Using Raman Spectroscopy (2009)

Charged impurity (CI) scattering is one of the dominant factors that affects the carrier mobility in graphene. In this paper, we use Raman spectroscopy to probe the charged impurities in suspended graphene. We find that the 2D band intensity is very sensitive to the CI concentration in graphene, while the G band intensity is not affected. The intensity ratio between the 2D and G bands, I(2D)/I(G), of suspended graphene is much stronger compared to that of nonsuspended graphene, due to the extremely low CI concentration in the former. This finding is consistent with the ultrahigh carrier mobility in suspended graphene observed in recent transport measurements. Our results also suggest that at low CI concentrations that are critical for device applications, the I(2D)/I(G) ratio is a better criterion in selecting high quality single layer graphene samples than is the G band blue shift.
Lack of correlation between EBV serology and presence of EBV in the Hodgkin and Reed-Sternberg cells of patients with Hodgkin's disease (1997)

Epstein-Barr virus (EBV) is detected in Hodgkin and Reed-Sternberg (HRS) cells in up to 50% of patients with Hodgkin's disease (HD). HD patients have been reported to express high serum titers against EBV antigens, even prior to the diagnosis of HD. Patients with high serum titers have a poorer prognosis. The aim of this study was to examine the relationship between the presence of EBV in HRS cells and the antibody titers reactive with different EBV antigens. Frozen serum and histopathological tissues were available from 107 untreated HD patients diagnosed between 1979 and 1991. The presence of EBV in the HRS cells was evaluated with immunohistochemistry directed against the LMP-1 antigen and/or with in situ hybridization of EBER-1. Analyses were performed of serum titers against early antigen (EA), diffuse (IgA and IgG) and restricted (IgG), virus-capsid antigen (VCA) (IgA and IgG), and EBV-encoded nuclear antigens (EBNA, EBNA 1, EBNA 2A, EBNA 2B, EBNA 6). EBV was detected in 27/107 (25%) tumor specimens, with a higher proportion in the MC group 8/13 (62%) (p < 0.01). IgG VCA and EBNA were detected in 99/107 (93%), evidence of a previous EBV infection. There were no significant relationships between antibody titers reactive with different EBV antigens and detectable EBV in HRS cells. Furthermore, there did not appear to be any relationship between EBV serology or the presence of EBV in HRS cells and clinical outcome. The role of EBV in the development of HD, especially its relationship to the immunological response, remains unclear. Int. J. Cancer 72:394–397, 1997. © 1997 Wiley-Liss, Inc.
A care plan for self-administration of epoetin alfa. (1991)

A care plan was developed to assist nephrology nurses managing the care of patients self-administering epoetin alfa. This is a generic care plan intended to serve as a basis for communication and should be adapted for appropriateness to individual facilities.
Context switching system and architecture for intelligence, surveillance, and reconnaissance (2012)

Given the increasing utilization and dependence on ISR information, operators and imagery analysts monitoring intelligence feeds seek a capability to reduce processing overload in transformation of ISR data to actionable information. The objective they seek is improvement in time critical targeting (TCT) and response time for mission events. Existing techniques addressing this problem are inflexible and lack a dynamic environment for adaptation to changing mission events. This paper presents a novel approach to ISR information collection, processing, and response, called the ISR Context Switching System (ISR-CSS). ISR-CSS enables ground, sea, and airborne sensors to perform preliminary analysis and processing of data automatically at the platform before transferring actionable information back to ground-base operators and intelligence analysts. The on-platform processing includes a catalogue of filtering algorithms concatenated with associated compression algorithms that are automatically selected based on dynamic mission events. The filtering algorithms employ tunable parameters and sensitivities based on the original mission plan along with associated Essential Elements of Information (EEI), data type, and analyst/user preferences. As a mission progresses, ISR-CSS incorporates adaptive parameter updates (model-based, statistics-based, learning-based, and event-driven), providing increased tactical relevant data. If a mission transforms dramatically, where unexpected manual guidance is required, then ISR-CSS allows tactical end-user direct-to-sensor tasking. To address information overload, ISR-CSS provides the provision to filters and prioritize data according to end-user preferences. ISR-CSS dispenses mission-critical and timely actionable information for end-user utilization, enabling faster response to a greater range of threats across the mission spectrum.
Cytochrome c release from mitochondria proceeds by a two-step process (2002)

Cytochrome c is often released from mitochondria during the early stages of apoptosis, although the precise mechanisms regulating this event remain unclear. In this study, with isolated liver mitochondria, we demonstrate that cytochrome c release requires a two-step process. Because cytochrome c is present as loosely and tightly bound pools attached to the inner membrane by its association with cardiolipin, this interaction must first be disrupted to generate a soluble pool of this protein. Specifically, solubilization of cytochrome c involves a breaching of the electrostatic and/or hydrophobic affiliations that this protein usually maintains with cardiolipin. Once cytochrome c is solubilized, permeabilization of the outer mitochondrial membrane by Bax is sufficient to allow the extrusion of this protein into the extramitochondrial environment. Neither disrupting the interaction of cytochrome c with cardiolipin, nor permeabilizing the outer membrane with Bax, alone, is sufficient to trigger this protein's release. This mechanism also extends to conditions of mitochondrial permeability transition insofar as cytochrome c release is significantly depressed when the electrostatic interaction between cytochrome c and cardiolipin remains intact. Our results indicate that the release of cytochrome c involves a distinct two-step process that is undermined when either step is compromised.
Low-Temperature Processed Electron Collection Layers of Graphene/TiO2 Nanocomposites in Thin Film Perovskite Solar Cells (2013)

The highest efficiencies in solution-processable perovskite-based solar cells have been achieved using an electron collection layer that requires sintering at 500 °C. This is unfavorable for low-cost production, applications on plastic substrates, and multijunction device architectures. Here we report a low-cost, solution-based deposition procedure utilizing nanocomposites of graphene and TiO2 nanoparticles as the electron collection layers in meso-superstructured perovskite solar cells. The graphene nanoflakes provide superior charge-collection in the nanocomposites, enabling the entire device to be fabricated at temperatures no higher than 150 °C. These solar cells show remarkable photovoltaic performance with a power conversion efficiency up to 15.6%. This work demonstrates that graphene/metal oxide nanocomposites have the potential to contribute significantly toward the development of low-cost solar cells.
Gels of syndiotacticity-rich poly(vinyl alcohol)–water/dimethyl sulfoxide or – water/ethylene glycol solutions (1987)

Gels of syndiotacticity-rich poly(vinyl alcohol) (s-PVA) in mixed solvents of water/dimethyl sulfoxide (DMSO) or water/ethylene glycol (EG) were made by chilling at the temperatures of 0–70°C from those solutions with the polymer concentrations below 10g/dL. The melting points of the gels were measured warming the gel from the gelling temperature (Tgel) at a constant heating rate. The apparent enthalpy of fusion of a junction of gel, ΔH was estimated from the relation between the apparent melting temperature and the polymer concentration. The s-PVA gels made from the mixtures of the water/lower contents of DMSO or EG had a minimum at lower Tgel and a maximum ΔH at a higher Tgel. On the other hand, the s-PVA gels made from the mixtures of the water/higher contents of them had nearly a maximum ΔH at a higher Tgel. From those results, it was considered that the former gels received a high thermal history while the latter gels received only slight thermal history.
Alternative solutions to hCG induction of ovulation in the mare. (1987)

Injection of hCG (2000-2500 i.u., i.v.) to mares when a follicle reaches 35 mm induces ovulation between 24 and 48 h. However, repeated injections induce antibodies against hCG. We report attempts to induce ovulation without this inconvenience. We called 'response' an ovulation between 24 and 48 h after treatment. The typical response to hCG was obtained in 73% (N = 145) of treated mares. After immunization against hCG, the response (0%, N = 10) was less than in nonimmunized controls (100%, N = 9). Simultaneous injection of dexamethasone and hCG resulted in induction of ovulation (71%, N = 14). However, simultaneous hCG + dexamethasone resulted in antibody formation similar to that induced by hCG alone, when injected repeatedly every 21 days. Neither GnRH (2 mg i.m.) nor partly purified pig LH (26 mg i.v. or s.c.) induced ovulation consistently (40%, N = 30 and 31%, N = 16). Crude horse gonadotrophin (60 mg i.v. or s.c.) induced ovulation (86%, N = 14). Fertility was not different from control (61%, N = 13 vs 40%, N = 10). Crude horse gonadotrophin also induced ovulation in mares previously immunized against hCG (78%, N = 9): 50 or 25 mg gave satisfactory response (86%, N = 29 and 57%, N = 40). We conclude that crude horse gonadotrophin is a good alternative to hCG for the induction of ovulation in mares.
Insulin nanoparticles for transdermal delivery: preparation and physicochemical characterization and in vitro evaluation (2010)

These results suggest that insulin nanoparticles can have a great potential in TDD systems of diabetes chemotherapy.
Protease-Activated Receptor 2 Mediates Eosinophil Infiltration and Hyperreactivity in Allergic Inflammation of the Airway (2002)

Trypsin and mast cell tryptase can signal to epithelial cells, myocytes, and nerve fibers of the respiratory tract by cleaving proteinase-activated receptor 2 (PAR2). Since tryptase inhibitors are under development to treat asthma, a precise understanding of the contribution of PAR2 to airway inflammation is required. We examined the role of PAR2 in allergic inflammation of the airway by comparing OVA-sensitized and -challenged mice lacking or overexpressing PAR2. In wild-type mice, immunoreactive PAR2 was detected in airway epithelial cells and myocytes, and intranasal administration of a PAR2 agonist stimulated macrophage infiltration into bronchoalveolar lavage fluid. OVA challenge of immunized wild-type mice stimulated infiltration of leukocytes into bronchoalveolar lavage and induced airway hyperreactivity to inhaled methacholine. Compared with wild-type animals, eosinophil infiltration was inhibited by 73% in mice lacking PAR2 and increased by 88% in mice overexpressing PAR2. Similarly, compared with wild-type animals, airway hyperreactivity to inhaled methacholine (40 micro g/ml) was diminished 38% in mice lacking PAR2 and increased by 52% in mice overexpressing PAR2. PAR2 deletion also reduced IgE levels to OVA sensitization by 4-fold compared with those of wild-type animals. Thus, PAR2 contributes to the development of immunity and to allergic inflammation of the airway. Our results support the proposal that tryptase inhibitors and PAR2 antagonists may be useful therapies for inflammatory airway disease.
Two Distinct Broadly Neutralizing Antibody Specificities of Different Clonal Lineages in a Single HIV-1-Infected Donor: Implications for Vaccine Design (2012)

Plasma from a small subset of subjects chronically infected with HIV-1 shows remarkable magnitude and breadth of neutralizing activity. From one of these individuals (CH0219), we isolated two broadly neutralizing antibodies (bnAbs), CH01 and VRC-CH31, from two clonal lineages of memory B cells with distinct specificities (variable loop 1 and 2 [V1V2] conformational specificity and CD4-binding site specificity, respectively) that recapitulate 95% of CH0219 serum neutralization breadth. These data provide proof of concept for an HIV-1 vaccine that aims to elicit bnAbs of multiple specificities.
Remdesivir Treatment for COVID 19 in Pregnant Patients with Moderate to Severe Symptoms: Serial Case Report (2021)

Remdesivir protocol for pregnant women with moderate to severe symptoms of COVID-19 has resulted in better clinical improvement with a shorter recovery period and no adverse effects during the hospitalization period. Further studies and RCT are warranted to evaluate the biosafety and effects of Remdesivir in pregnant women.
Electronic spectra of polysilanes (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectronic spectra of polysilanesL. A. Harrah and J. M. ZeiglerCite this: Macromolecules 1987, 20, 3, 601–608Publication Date (Print):March 1, 1987Publication History Published online1 May 2002Published inissue 1 March 1987https://doi.org/10.1021/ma00169a023RIGHTS & PERMISSIONSArticle Views263Altmetric-Citations161LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (1 MB) Get e-Alerts Get e-Alerts
Concomitant neutrophil JAK2V617F mutation screening and PRV‐1 expression analysis in myeloproliferative disorders and secondary polycythaemia (2005)

Polycythaemia vera (PV) is closely associated with both an acquired activating mutation of the JAK2 tyrosine kinase (JAK2(V617F)) in granulocyte-derived DNA and increased granulocyte polycythaemia rubra vera-1 (PRV-1) expression. In order to explore the correlation between these two biological markers and compare their diagnostic utility, mutation analysis for JAK2(V617F) and quantitative measurement of granulocyte PRV-1 expression were performed on the same study sample from 100 participants: 38 with PV, 22 with essential thrombocythaemia (ET), 10 with agnogenic myeloid metaplasia (AMM), 19 with secondary polycythaemia (SP) and 11 healthy volunteers. The respective overall (homozygous) JAK2(V617F) mutational frequencies were 95% (26%), 55% (0%), 30% (0%), 0% and 0%. The corresponding figures for increased PRV-1 expression were 89%, 18%, 20%, 21% and 9%. In patients with either ET or AMM, the likelihood of detecting JAK2(V617F) was significantly higher in the presence of an increased PRV-1 expression (83% vs. 38%; P = 0.05). Similarly, in patients with PV, homozygous as compared with heterozygous JAK2(V617F) correlated with higher levels of PRV-1 expression (P = 0.11). The present study suggests an allele dose-dependent effect of JAK2(V617F) on granulocyte PRV-1 expression. However, compared with the PRV-1 assay, mutation screening for JAK2(V617F) displayed greater accuracy in distinguishing PV from SP.
Antigenic structures recognized by cytotoxic T lymphocytes (1991)

cells, through antibody molecules embedded in their cell surface membrane, collectively recognize an enormous diver- sity of antigens in solution or on cell surfaces, including native and unfolded proteins, peptides, polysaccharides, nucleic acids, steroids, and small organic molecules. The antigen- specific receptors on T cells are very similar to antibodies structurally and in the genetic basis for their diversity. Never- theless, the antigenic structures recognized by T cells consist almost exclusively of peptides associated with integral mem- brane glycoproteins known as MHC’ proteins (because they are encoded in a genetic region called the major histocompat- ibility complex). Thus T cells recognize protein antigens in a fragmented (or “processed”) form on the surface of other cells expressing appropriate MHC molecules (a phenomenon termed MHC “restriction” of the T cell response
The New State of the Art: Cas9 for Gene Activation and Repression (2015)

CRISPR-Cas9 technology has rapidly changed the landscape for how biologists and bioengineers study and manipulate the genome. Derived from the bacterial adaptive immune system, CRISPR-Cas9 has been coopted and repurposed for a variety of new functions, including the activation or repression of gene expression (termed CRISPRa or CRISPRi, respectively). This represents an exciting alternative to previously used repression or activation technologies such as RNA interference (RNAi) or the use of gene overexpression vectors. We have only just begun exploring the possibilities that CRISPR technology offers for gene regulation and the control of cell identity and behavior. In this review, we describe the recent advances of CRISPR-Cas9 technology for gene regulation and outline advantages and disadvantages of CRISPRa and CRISPRi (CRISPRa/i) relative to alternative technologies.
Multifunctional protein processing chip with integrated digestion, solid‐phase extraction, separation and electrospray (2010)

We describe a microfluidic device in which integrated tryptic digestion, SPE, CE separation and electrospray ionization for MS are performed. The chip comprised of 10 × 30 μm channels for CE, and two serially connected 150 μm deep, 800 μm wide channels packed with 40 to 60 μm diameter beads, loaded with either immobilized trypsin, reversed-phase packing or both. On-chip digestion of cytochrome c using the trypsin bed showed complete consumption of the protein in 3 min, in contrast to the 2 h required for conventional solution phase tryptic digestion. SPE of 0.25 μg/mL solutions of the peptides leu-enkephalin, angiotensin II and LHRH gave concentration enhancements in the range of 4.4-12, for a ten times nominal volume ratio. A 100 nM cytochrome c sample concentrated 13.3 times on-chip gave a sequence coverage of 85.6%, with recovery values ranging from 41.2 to 106%. The same sample run without SPE showed only five fragment peaks and a sequence coverage of 41.3%. When both on-chip digestion and SPE (13.3 volume ratio concentration enhancement) were performed on 200 nM cytochrome c samples, a sequence coverage of 76.0% and recovery values of 21-105% were observed. Performing on-chip digestion alone on the same sample gave only one significant fragment peak. The above digestion/peptide concentration step was compared to on-chip protein concentration by SPE followed by on-chip digestion with solution phase trypsin. Both procedures gave similar recovery results; however, much larger trypsin autodigestion interference in the latter approach was apparent.
Large induction of keratinocyte growth factor expression in the dermis during wound healing. (1992)

Recent studies have shown that application of basic fibroblast growth factor (basic FGF) to a wound has a beneficial effect. However, it has not been assessed whether endogenous FGF also plays a role in tissue repair. In this study we found a 160-fold induction of mRNA encoding keratinocyte growth factor (KGF) 1 day after skin injury. This large induction was unique within the family of FGFs, since mRNA levels of acidic FGF, basic FGF, and FGF-5 were only slightly induced (2- to 10-fold) during wound healing, and there was no expression of FGF-3, FGF-4, and FGF-6 detected in normal and wounded skin. High levels of FGF receptor 1 and FGF receptor 2 mRNA and low levels of FGF receptor 3 mRNA were found in both normal and wounded skin. No change in the levels of these transcripts was detected during wound healing. In situ hybridization studies revealed highest levels of KGF mRNA expression in the dermis at the wound edge and in the hypodermis below the wound. In contrast, mRNA encoding the receptor of this growth factor (a splice variant of FGF receptor 2) was predominantly expressed in the epidermis. These results suggest that basal keratinocytes are stimulated by dermally derived KGF during wound healing and implicate a unique role of this member of the FGF family in wound repair.
Nab-Paclitaxel Is an Active Drug in Preclinical Model of Pediatric Solid Tumors (2013)

Nab-paclitaxel showed significant antitumor activity against all pediatric solid tumors associated with an enhanced drug intratumor delivery. Furthermore, testing of nab-paclitaxel in pediatric solid-tumor patient population is under development.