gebhart/patrepeval huggingface dataset:
Self-Transmissible Mercury Resistance Plasmids with Gene-Mobilizing Capacity in Soil Bacterial Populations: Influence of Wheat Roots and Mercury Addition (1998)

A set of mercury resistance plasmids was obtained from wheat rhizosphere soil amended or not amended with mercuric chloride via exogenous plasmid isolation by using Pseudomonas fluorescens R2f, Pseudomonas putida UWC1, and Enterobacter cloacae BE1 as recipient strains. The isolation frequencies were highest from soil amended with high levels of mercury, and the isolation frequencies from unamended soil were low. With P. putida UWC1 as the recipient, the isolation frequency was significantly enhanced in wheat rhizosphere compared to bulk soil. Twenty transconjugants were analyzed per recipient strain. All of the transconjugants contained plasmids which were between 40 and 50 kb long. Eight selected plasmids were distributed among five groups, as shown by restriction digestion coupled with a similarity matrix analysis. However, all of the plasmids formed a tight group, as judged by hybridization with two whole-plasmid probes and comparisons with other plasmids in dot blot hybridization analyses. The results of replicon typing and broad-host-range incompatibility (Inc) group-specific PCR suggested tht the plasmid isolates were not related to any previously described Inc group. Although resistance to copper, resistance to streptomycin, and/or resistance to chloramphenicol was found in several plasmids, catabolic sequences were generally not identified. One plasmid, pEC10, transferred into a variety of bacteria belonging to the beta and gamma subdivisions of the class Proteobacteria and mobilized as well as retromobilized the IncQ plasmid pSUP104. A PCR method for detection of pEC10-like replicons was used, in conjunction with other methods, to monitor pEC10-homologous sequences in mercury-polluted and unpolluted soils. The presence of mercury enhanced the prevalence of pEC10-like replicons in soil and rhizosphere bacterial populations.
Protic Ionic Liquids:  Properties and Applications (2007)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProtic Ionic Liquids: Properties and ApplicationsTamar L. Greaves and Calum J. DrummondView Author Information CSIRO Molecular and Health Technologies (CMHT), Bag 10, Clayton, Vic 3169, Australia, and CSIRO Materials Science and Engineering (CMSE), Private Bag 33, Clayton MDC, Vic 3169, Australia Cite this: Chem. Rev. 2008, 108, 1, 206–237Publication Date (Web):December 21, 2007Publication History Received4 October 2006Published online21 December 2007Published inissue 1 January 2008https://pubs.acs.org/doi/10.1021/cr068040uhttps://doi.org/10.1021/cr068040uresearch-articleACS PublicationsCopyright © 2008 American Chemical SocietyRequest reuse permissionsArticle Views26964Altmetric-Citations2060LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Anions,Catalysts,Cations,Salts,Solvents Get e-Alerts
β‐Alanine containing peptides: γ‐Turns in cyclotetrapeptides (1993)

In the present paper we describe the synthesis, purification, single-crystal x-ray analysis, solution conformational characterization, and conformational energy calculations of the cyclic tetrapeptide cyclo-(beta-Ala-L-Pro-beta-Ala-L-Val). The peptide was synthesized by classical solution methods and the cyclization of the free tetrapeptide was accomplished in good yields in diluted methylene chloride solution using N,N-dicyclohexyl-carbodiimide. The compound crystallizes in the monoclinic space group P2(1) from ethanol with two independent molecules in the unit cell. All peptide bonds are trans. The nmr molecular conformation in the acetonitrile solution as well as that derived from the molecular dynamic simulation in vacuo is quite different from those observed in the solid state and is very similar to that previously observed for the parent compound cyclo- (beta-Ala-L-Pro-beta-Ala-L-Pro).
The Embryo and Its Future1 (2004)

The preimplantation mammalian embryo from different species appears sensitive to the environment in which it develops, either in vitro or in vivo, for example, in response to culture conditions or maternal diet. This sensitivity may lead to long-term alterations in the characteristics of fetal and/or postnatal growth and phenotype, which have implications for clinical health and biotechnological applications. We review the breadth of environmental influences that may affect early embryos and their responses to such conditions along epigenetic, metabolic, cellular, and physiological directions. In addition, we evaluate how embryo environmental responses may influence developmental potential and phenotype during later gestation. We conclude that a complex of different mechanisms may operate to associate early embryo environment with future health.
Human Gene Therapy (1992)

Human gene therapy is a procedure that is being used in an attempt to treat genetic and other diseases. Eleven clinical protocols are under way at the present time, each with scientific and clinical objectives. Human genetic engineering raises unique safety, social, and ethical concerns.
Structural and spectroscopic properties of a copper(I)–bis(N-heterocyclic)carbene complex (2009)

The structural and spectroscopic properties of a Cu(I) complex bearing a methylene-linked bis(N-heterocyclic carbene) ligand, [Cu(2)(mu-Me-mbim)(2)](PF(6))(2) were investigated. X-ray single crystal structure analysis revealed that the complex is binuclear similar to the corresponding silver(I) complex. In [Cu(2)(mu-Me-mbim)(2)](PF(6))(2), cation-pi interaction between copper and the adjacent carbene carbon is observed. On the other hand, the copper-copper interaction is very weak in the crystal and almost negligible in solution. The absorption spectrum of [Cu(2)(mu-Me-mbim)(2)](PF(6))(2) in methanol shows a strong absorption band (epsilon = 23 000 dm(3) mol(-1) cm(-1)) and a weaker shoulder (epsilon = 6200 dm(3) mol(-1) cm(-1)) at 261 nm and 300 nm, respectively. From molecular orbital calculations using TD-DFT, these absorption bands are assigned to the metal-centered transitions with some contribution from the NHC orbitals. The powdered sample of [Cu(2)(mu-Me-mbim)(2)](PF(6))(2) shows bright blue-green phosphorescence with a high quantum yield (43%). The phosphorescence is of dual-emission character at room temperature with peak maxima at 374 nm and 482 nm whereas it changes to a single emission band centered around 500 nm at 77 K. Molecular orbital calculations indicate that the luminescence derives from the triplet MC and MLCT mixed excited states. A methanolic solution of [Cu(2)(mu-Me-mbim)(2)](PF(6))(2) shows yellow-green phosphorescence with a peak maximum at 542 nm. Unlike in the solid state, no dual-emission was observed. These results suggest that the dual emission is caused by differences in the contribution of metal-metal interactions at room temperature in the solid state. The differences in the absorption and emission properties between [Cu(2)(mu-Me-mbim)(2)](PF(6))(2) and the related Cu(I)-diphosphine complex, [Cu(2)(mu-dcpm)(2)](BF(4))(2) are discussed.
Identification of a new serine kinase that activates NF kappa B by direct phosphorylation. (1993)

A novel serine kinase, named nuclear factor kappa B (NF kappa B) kinase, has been shown to be associated with the NF kappa B.I kappa B complex in the cytosol of human primary T lymphocytes. It activates the DNA binding activity of NF kappa B by directly phosphorylating its p65 and p50 subunits. There is no evidence that it phosphorylates I kappa B. Experiments with inhibitors and antisera showed that it is distinct from other known serine kinases such as mitogen-activated protein kinase or Mos. It has an apparent molecular size of 43 kDa determined by the substrate binding assay. This kinase might have a central role in mediating various signals to NF kappa B.
Dynamics of tissue oxygenation in isolated rabbit heart as measured with near-infrared spectroscopy (1999)

We investigated the role of myoglobin (Mb) in supplying O2 to mitochondria during transitions in cardiac workload. Isovolumic rabbit hearts (n = 7) were perfused retrogradely with hemoglobin-free Tyrode solution at 37 degrees C. Coronary venous O2 tension was measured polarographically, and tissue oxygenation was measured with two-wavelength near-infrared spectroscopy (NIRS), both at a time resolution of approximately 2 s. During transitions to anoxia, 68 +/- 2% (SE) of the NIRS signal was due to Mb and the rest to cytochrome oxidase. For heart rate steps from 120 to 190 or 220 beats/min, the NIRS signal decreased significantly by 6.9 +/- 1.3 or 11.1 +/- 2.1% of the full scale, respectively, with response times of 11.0 +/- 0.8 or 9.1 +/- 0.5 s, respectively. The response time of end-capillary O2 concentration ([O2]), estimated from the venous [O2], was 8.6 +/- 0.8 s for 190 beats/min (P < 0.05 vs. NIRS time) or 8.5 +/- 0.9 s for 220 beats/min (P > 0.05). The mean response times of mitochondrial O2 consumption (VO2) were 3.7 +/- 0.7 and 3.6 +/- 0.6 s, respectively. The deoxygenation of oxymyoglobin (MbO2) accounted for only 12-13% of the total decrease in tissue O2, with the rest being physically dissolved O2. During 11% reductions in perfusion flow at 220 beats/min, Mb was 1.5 +/- 0.4% deoxygenated (P < 0.05), despite the high venous PO2 of 377 +/- 17 mmHg, indicating metabolism-perfusion mismatch. We conclude that the contribution of MbO2 to the increase of VO2 during heart rate steps in saline-perfused hearts was small and slow compared with that of physically dissolved O2.
Implementation of adaptive 5400 bit/s modem over frequency selective HF radio links (1992)

The aim is to design, build and test a practical voiccband HF modem that can cope with frequency selective fading. The main objective is to implement the most cost effective system employing the available serial and parallel signal processor devices that can deal with the appropriate detection algorithms as well as simulate the HF channel itself.
Tryptophan 388 in Putative Transmembrane Segment 10 of the Rat Glucose Transporter Glut1 Is Essential for Glucose Transport (1998)

The molecular mechanism of substrate recognition in membrane transport is not well understood. Two amino acid residues, Tyr446 and Trp455 in transmembrane segment 10 (TM10), have been shown to be important for galactose recognition by the yeast Gal2 transporter; Tyr446 was found to be essential in that its replacement by any of the other 19 amino acids abolished transport activity (Kasahara, M., Shimoda, E., and Maeda, M. (1997) J. Biol. Chem. 272, 16721-16724). The Glut1 glucose transporter of animal cells belongs to the same Glut transporter family as does Gal2 and thus might be expected to show a similar mechanism of substrate recognition. The role of the two amino acids, Phe379 and Trp388, in rat Glut1 corresponding to Tyr446 and Trp455 of Gal2 was therefore studied. Phe379 and Trp388 were individually replaced with each of the other 19 amino acids, and the mutant Glut1 transporters were expressed in yeast. The expression level of most mutants was similar to that of the wild-type Glut1, as revealed by immunoblot analysis. Glucose transport activity was assessed by reconstituting a crude membrane fraction of the yeast cells in liposomes. No significant glucose transport activity was observed with any of Trp388 mutants, whereas the Phe379 mutants showed reduced or no activity. These results indicate that the two aromatic amino acids in TM10 of Glut1 are important for glucose transport. However, unlike Gal2, the residue at the cytoplasmic end of TM10 (Trp388, corresponding to Trp455 of Gal2), rather than that in the middle of TM10 (Phe379, corresponding to Tyr446 of Gal2), is essential for transport activity.
Venezuelan equine encephalitis‐specific immunoglobulin responses: Live attenuated TC‐83 versus inactivated C‐84 vaccine (1992)

Venezuelan equine encephalitis (VEE)-specific immunoglobulin responses to the two vaccines, TC-83 (a live attenuated vaccine) and C-84 (a formalin inactivated vaccine derived from the TC-83 strain of virus) were evaluated using an antigen and isotype-specific enzyme-linked immunoadsorbent assay (ELISA). The VEE-specific ELISA for IgG, IgG subclasses, IgA and IgM were developed and standardized using sera from vaccine-exposed and unexposed human subjects. Paired human sera (before and 28 days after immunization) were tested from laboratory workers vaccinated with either TC-83 (Group A: 20 paired sera from subjects receiving a single TC-83 vaccine and with no prior history of vaccination) or C-84 in varying schedules (Group B: 19 paired sera from subjects who had a distant vaccination history to TC-83 but no evidence of neutralizing antibody; Group C: 19 paired sera from subjects receiving their first C-84 vaccination and no prior documented history of vaccination; Group D: 15 paired sera from subjects receiving a C-84 booster vaccination with prior history of C-84 but no TC-83 exposure). Sera were all tested for viral neutralization in vitro using a Vero cell monolayer for culturing virus and establishing 80% plaque reduction for each serum tested. All pre-sera tested demonstrated no plaque reduction neutralization at a level of 80% for a dilution of 1:10. ELISA antibody titers for all pre-sera with no prior VEE exposure through vaccination or possible environmental factors were negative at a titer of 1:160 for IgM, 1:80 for IgG, IgA, and G subclasses.(ABSTRACT TRUNCATED AT 250 WORDS)
Agent-based Intelligent Control System Design for Real-time Distributed Manufacturing Environments (1998)

This research focuses on development of a generic agent-based intelligent control system designer for realtime distributed manufacturing environments. The paper first describes related research into distributed manufacturing and real-time control system, then reports on the intelligent control function design interface and automatic control code generation. Keywords: Multi-agent, manufacturing, real-time control, function block, automatic code generation. 1. INTRODUCTION Over the last several decades, computer control of manufacturing systems has been the focus of extensive research. Advances in microprocessor, computing, networking and interfacing technologies have improved capabilities of industrial automation and control systems substantially over this period. However, these control systems are proprietary and still have problems in areas such as interoperability, scalability, and lack of standard user interfaces. The development of open architecture control systems addresses som...
Using R in Taverna: RShell v1.2 (2009)

Our RShell plugin enables Taverna users to employ R scripts within their workflows in a highly configurable way.
Synthetic stereo acoustic echo cancellation structure with microphone array beamforming for VoIP conferences (2002)

This paper proposes a synthetic stereo acoustic echo cancellation structure with microphone array beamforming for multiple participant voice over Internet protocol (VoIP) conferencing. The proposed acoustic front-end combines a synthetic stereo structure, acoustic echo canceler and microphone array beamformer. Synthetic stereo is used to add spatial information to signals from multiple participants that facilitates distinguishing between participants. Microphone array beamforming is then combined with the single canceler per spatial region echo cancellation structure to reduce noise and reverberation for near-end speech pickup and to cancel echo.
A 32 kDa lipocortin from human mononuclear cells appears to be identical with the placental inhibitor of blood coagulation (1989)

A 32 kDa protein isolated from human mononuclear cells is a member of the lipocortin family, a new group of Ca2+-dependent lipid-binding proteins thought to be involved in the regulation of phospholipase A2, in exocytosis and in membrane-cytoskeleton interactions. Purification of this protein was based on its ability to associate with membrane phospholipids in a Ca2+-dependent manner and its capacity to inhibit purified phospholipase A2 from pig pancreas. Using immunological detection, we show that it is present in various cells involved in the inflammatory and coagulation processes. We present extensive amino acid data that strongly suggest that this protein is identical with a recently described inhibitor of blood coagulation, with endonexin II and with lipocortin V. Sequence alignment with other known proteins show a significant degree of homology with lipocortins I and II, the substrates of the epidermal-growth-factor receptor tyrosine kinase and the oncogene pp60src tyrosine kinase respectively, and with protein II. The possible physiological role of this 32 kDa lipocortin is discussed.
Synthesis of imidazo[1,2‐b]pyridazines: Fenbendazole, oxifenbendazole analogs and related derivatives (1993)

Abstract A series of imidazo[1,2‐ b ]pyridazines has been prepared and evaluated for macrofilaricidal activity against Brugia pahangi or Acanthocheilonema viteae infections in jirds. The imidazo[1,2‐ b ]pyridazine analogs of fen‐bendazole and oxifenbendazole are reported. In addition, several 6‐aminoimidazo[1,2‐ b ]pyridazine derivatives have been prepared. None of these compounds possessed significant activity against human cytomega‐lovirus (HCMV) or filarial infections.
Structural analysis of inhibition of E. coli methionine aminopeptidase: implication of loop adaptability in selective inhibition of bacterial enzymes (2007)

The adaptability of the YHGY loop in E. coli methionine aminopeptidase, and likely in other bacterial methionine aminopeptidases, enables the enzyme active pocket to accommodate inhibitors of differing size. The differences in this adaptable loop between the bacterial and human methionine aminopeptidases is a structural feature that can be exploited to design inhibitors of bacterial methionine aminopeptidases as therapeutic agents with minimal inhibition of the corresponding human enzymes.
Inhibition of Caspase-mediated Anoikis Is Critical for Basic Fibroblast Growth Factor-sustained Culture of Human Pluripotent Stem Cells (2009)

Apoptosis and proliferation are two dynamically and tightly regulated processes that together maintain the homeostasis of renewable tissues. Anoikis is a subtype of apoptosis induced by detachment of adherent cells from the extracellular matrix. By using the defined mTeSR1 medium and collecting freshly detached cells, we found here that human pluripotent stem (PS) cells including embryonic stem (ES) cells and induced pluripotent stem cells are subject to constant anoikis in culture, which is escalated in the absence of basic fibroblast growth factor (bFGF). Withdrawal of bFGF also promotes apoptosis and differentiation of the remaining adherent cells without affecting their cell cycle progression. Insulin-like growth factor 2 (IGF2) has previously been reported to act downstream of FGF signaling to support self-renewal of human ES cells. However, we found that IGF2 cannot substitute bFGF in the TeSR1-supported culture, although endogenous IGF signaling is required to sustain self-renewal of human ES cells. On the other hand, all of the bFGF withdrawal effects observed here can be markedly prevented by the caspase inhibitor z-VAD-FMK. We further demonstrated that the bFGF-repressed anoikis is dependent on activation of ERK and AKT and associated with inhibition of Bcl-2-interacting mediator of cell death and the caspase-ROCK1-myosin signaling. Anoikis is independent of pre-detachment apoptosis and differentiation of the cells. Because previous studies of human PS cells have been focused on attached cells, our findings revealed a neglected role of bFGF in sustaining self-renewal of human PS cells: preventing them from anoikis via inhibition of caspase activation.
Oral infection of Aedes polynesiensis by Wuchereria bancrofti by using parafilm membrane feeding. (1991)

In order to construct a cDNA library from third-stage larvae (L3) of Wuchereria bancrofti var. pacifica, the Parafilm membrane feeding method is proposed for the oral infection of Aedes polynesiensis. Heparinized blood supplemented with 5.10(-3) M ATP was put in the feeder with carbon dioxide provided as additional phagostimulant. The results of this artificial infection feeding method were compared with those obtained when mosquitoes fed directly on the forearm of a microfilaremic patient. The number of females feeding through the artificial membrane was smaller than on the patient's forearm (32.1 vs. 84.8%). The mean number of L3s obtained per female was not statistically different between the 2 feeding methods; however, the total number of L3s obtained from 100 females allowed to feed in each group was twice as high in the natural feeding method.
Lithium batteries : new materials, developments, and perspectives (1994)

Carbons and graphites as substitutes for the lithium anode, J.R. Dahn et al electrode materials based on carbon and graphite intercalation compounds in liquid and polymeric electrolytes, R. Yazami room-temperature polymer electrolytes, M. Alamgir and K.M. Abraham current state of the art on lithium battery electrolytes, L.A. Dominey thin film technology and microbatteries, C. Julien four-volt cathodes for lithium accumulators and the Li-ion battery concept, T. Ohzuku cathode materials synthesized by low temperature techniques, J.P. Pereira-Ramos et al solid-state sodium batteries, K. West comparison of high-power ambient-temperature cells, P. Chenebault implantable lithium power sources, C.F. Holmes commercial cells based on MnO[2] and MnO[2]-related cathodes, T. Nohma et al intercalation in layered and three-dimensional oxides, C. Delmas.
A 660 MB/s interface megacell portable circuit in 0.3 μm-0.7 μm CMOS ASIC (1996)

A byte-wide I/O cell with 660 MB/s data rate uses low-jitter DLL-generated clocks, self-calibrated controlled-current drivers, and linear amplifier input receivers. The CAD techniques developed allow the design to be ported to CMOS processes ranging from 0.7 /spl mu/m to 0.3 /spl mu/m. The chip is 0.9/spl times/3.4 mm/sup 2/ using 0.3 /spl mu/m rules.