gebhart/patrepeval huggingface dataset:
Genetic alterations in bronchial mucosa and plasma DNA from individuals at high risk of lung cancer (2001)

Evidence suggests that the majority of lung cancer patients have tumour-derived genetic alterations in circulating plasma DNA, and that this may be developed as a diagnostic tool. To this end, we have studied 60 individuals attending bronchoscopy clinic, with symptoms suspicious of lung cancer, for genetic alterations in bronchial mucosa biopsy (n = 47) and plasma (n = 40) DNA. Thirteen of 47 individuals from whom biopsies were taken displayed allelic loss of heterozygosity (LOH) in biopsy DNA for at least 1 of 4 markers. All 13 of these individuals had neoplastic tumour cells in their biopsies and were subsequently diagnosed with cancer. Thirteen of 40 individuals from whom plasma was taken displayed a plasma DNA LOH, and 12 of these 13 individuals were subsequently diagnosed with cancer. LOH in plasma was generally representative of LOH in the corresponding biopsy. In terms of sensitivity, using just 4 markers, biopsy LOH and plasma LOH were found in 13 of 44 (30%) and 12 of 29 (41%), respectively, of those patients subsequently diagnosed with cancer. Two patients were positive for LOH in plasma samples that pre-dated a diagnosis of cancer by several months. These data suggest that assay of genetic alterations in circulating plasma DNA may be developed as a useful addition to conventional techniques for the diagnosis of lung cancer.
Peripheral blood eosinophilia in atopic dermatitis. (2007)

The results point to the role of eosinophils in etiopathogenesis of AD. Peripheral blood eosinophilia could serve as a diagnostic parameter in differentiating allergic AD from non-allergic AD.
Somatic Mutation and Light Chain Rearrangement Generate Autoimmunity in Anti–Single-Stranded DNA Transgenic Mrl/lpr Mice (1999)

Antibodies to single-stranded (ss)DNA are expressed in patients with systemic lupus erythematosus and in lupus-prone mouse models such as the MRL/Mp-lpr/lpr (MRL/lpr) strain. In nonautoimmune mice, B cells bearing immunoglobulin site-directed transgenes (sd-tgs) that code for anti-ssDNA are functionally silenced. In MRL/lpr autoimmune mice, the same sd-tgs are expressed in peripheral B cells and these autoantibodies gain the ability to bind other autoantigens such as double-stranded DNA and cell nuclei. These new specificities arise by somatic mutation of the anti-ssDNA sd-tgs and by secondary light chain rearrangement. Thus, B cells that in normal mice are anergic can be activated in MRL/lpr mice, which can lead to the generation of pathologic autoantibodies. In this paper, we provide the first direct evidence for peripheral rearrangement in vivo.
The Schistosome Oligosaccharide Lacto- N -neotetraose Expands Gr1+ Cells That Secrete Anti-inflammatory Cytokines and Inhibit Proliferation of Naive CD4+ Cells: A Potential Mechanism for Immune Polarization in Helminth Infections (2001)

Immunomodulatory oligosaccharides found on helminths also are found in human milk, and both helminths and milk have been shown to be immunosuppressive. We have been examining the immunomodulatory capabilities of two oligosaccharides expressed in milk and on helminth parasites, lacto-N-fucopentaose III and lacto-N-neotetraose (LNnT). In an attempt to dissect mechanisms that lead to Th2 polarization and immune suppression, we examined the early response in mice to the glycoconjugate LNnT-Dextran (LNnT-Dex). We found that injection of LNnT-Dex expanded a cell population, phenotypically defined as Gr1(+)/CD11b(+)/F4/80(+), as early as 2 h after injection. Examination of spontaneous cytokine production showed that this Gr1(+)/F4/80(+) population of cells spontaneously produced low levels of proinflammatory cytokines, but higher levels of IL-10 and TGF-beta ex vivo, compared to peritoneal cells from mice injected with Dex. Gr1(+) cells adoptively suppressed naive CD4(+) T cell proliferation in vitro in response to anti-CD3/CD28 Ab stimulation. Suppression of naive CD4(+) cells involved cell contact and was dependent on IFN-gamma and NO, with a discrete role played by IL-10. Coculture of naive CD4(+)T cells with Gr1(+) suppressor cells did not lead to CD4(+) T cell apoptosis, although it did imprint on naive CD4(+) T cells a response characterized by lower levels of IFN-gamma, coincident with increased IL-13 production. Our results suggest that both human milk and helminth parasites may share a ligand-specific mechanism involved in the generation of anti-inflammatory mediators that suppress Th1-type and inflammatory responses.
The effective drive current in CMOS inverters (2003)

A simple but accurate expression for the effective drive current, I/sub eff/, for CMOS inverter delay is obtained. We show that the choice I/sub eff/=(I/sub H/+I/sub L/)/2, where I/sub L/=I/sub ds/(V/sub gs/=V/sub dd//2,V/sub ds/=V/sub dd/), and I/sub H/=I/sub ds/(V/sub gs/=V/sub dd/,V/sub ds/=V/sub dd//2) is defined, accurately predicts inverter delay when tested against compact models over a variety of conditions and against hardware results in 90 nm node technology. Furthermore, this definition of I/sub eff/ accurately captures the delay behavior of non-traditionally scaled devices, where mobility and V/sub T//V/sub dd/ are scaled in neither a regular nor uniform manner.
Human T cell responses to Japanese encephalitis virus in health and disease (2016)

Japanese encephalitis (JE) virus (JEV) is an important cause of encephalitis in children of South and Southeast Asia. However, the majority of individuals exposed to JEV only develop mild symptoms associated with long-lasting adaptive immunity. The related flavivirus dengue virus (DENV) cocirculates in many JEV-endemic areas, and clinical data suggest cross-protection between DENV and JEV. To address the role of T cell responses in protection against JEV, we conducted the first full-breadth analysis of the human memory T cell response using a synthetic peptide library. Ex vivo interferon-γ (IFN-γ) responses to JEV in healthy JEV-exposed donors were mostly CD8(+) and targeted nonstructural (NS) proteins, whereas IFN-γ responses in recovered JE patients were mostly CD4(+) and targeted structural proteins and the secreted protein NS1. Among patients, a high quality, polyfunctional CD4(+) T cell response was associated with complete recovery from JE. T cell responses from healthy donors showed a high degree of cross-reactivity to DENV that was less apparent in recovered JE patients despite equal exposure. These data reveal divergent functional CD4(+) and CD8(+) T cell responses linked to different clinical outcomes of JEV infection, associated with distinct targeting and broad flavivirus cross-reactivity including epitopes from DENV, West Nile, and Zika virus.
Traumatic brain injury in the United States: an epidemiologic overview (2009)

A basic description of severity and frequency is needed for planning healthcare delivery for any disease process. In the case of traumatic brain injury, severity is typically categorized into mild, moderate, and severe with information from a combination of clinical observation and self-report methodologies. Recent US civilian epidemiological findings measuring the frequency of mortality and morbidity of traumatic brain injury are presented, including demographic and etiological breakdowns of the data. Falls, motor vehicle accidents, and being struck by objects are the major etiologies of traumatic brain injury. US civilian and Army hospitalization trends are discussed and compared. Features of traumatic brain injuries from Operation Iraqi Freedom and Operation Enduring Freedom are discussed.
Gender Preselection in Cattle with Intracytoplasmically Injected, Flow Cytometrically Sorted Sperm Heads1 (1999)

We investigated the development to the blastocyst and subsequent live-offspring stages of in vitro-matured bovine oocytes intracytoplasmically injected with flow cytometrically sorted bull sperm heads. Bull sperm heads, prepared by ultrasound sonication, were distinguished and sorted on the basis of their relative DNA contents using a flow cytometer/cell sorter modified for sorting sperm. By fluorescence in situ hybridization, the proportion of sperm confirmed as having Y specific DNA in the fraction sorted for the Y sperm was 82%. Injection with single sorted sperm heads of in vitro-matured oocytes (cultured for 24 h) resulted in 46.6% cleavage and 6.9% blastocyst development rates. Embryo transfer of 48 blastocysts (Days 7-8) to recipients (one per recipient) resulted in 20.8% pregnancy and 20.8% normal live offspring production rates. The birth of 8 male and 2 female calves represents an 80% sex preselection accuracy rate.
HIV-1 Nef mobilizes lipid rafts in macrophages through a pathway that competes with ABCA1-dependent cholesterol efflux (2012)

HIV infection, through the actions of viral accessory protein Nef, impairs activity of cholesterol transporter ABCA1, inhibiting cholesterol efflux from macrophages and elevating the risk of atherosclerosis. Nef also induces lipid raft formation. In this study, we demonstrate that these activities are tightly linked and affect macrophage function and HIV replication. Nef stimulated lipid raft formation in macrophage cell line RAW 264.7, and lipid rafts were also mobilized in HIV-1-infected human monocyte-derived macrophages. Nef-mediated transfer of cholesterol to lipid rafts competed with the ABCA1-dependent pathway of cholesterol efflux, and pharmacological inhibition of ABCA1 functionality or suppression of ABCA1 expression by RNAi increased Nef-dependent delivery of cholesterol to lipid rafts. Nef reduced cell-surface accessibility of ABCA1 and induced ABCA1 catabolism via the lysosomal pathway. Despite increasing the abundance of lipid rafts, expression of Nef impaired phagocytic functions of macrophages. The infectivity of the virus produced in natural target cells of HIV-1 negatively correlated with the level of ABCA1. These findings demonstrate that Nef-dependent inhibition of ABCA1 is an essential component of the viral replication strategy and underscore the role of ABCA1 as an innate anti-HIV factor.
Mean-shift blob tracking through scale space (2003)

The mean-shift algorithm is an efficient technique for tracking 2D blobs through an image. Although the scale of the mean-shift kernel is a crucial parameter, there is presently no clean mechanism for choosing or updating scale while tracking blobs that are changing in size. We adapt Lindeberg's (1998) theory of feature scale selection based on local maxima of differential scale-space filters to the problem of selecting kernel scale for mean-shift blob tracking. We show that a difference of Gaussian (DOG) mean-shift kernel enables efficient tracking of blobs through scale space. Using this kernel requires generalizing the mean-shift algorithm to handle images that contain negative sample weights.
Probabilistic visual learning for object detection (2002)

We present an unsupervised technique for visual learning which is based on density estimation in high-dimensional spaces using an eigenspace decomposition. Two types of density estimates are derived for modeling the training data: a multivariate Gaussian (for a unimodal distributions) and a multivariate Mixture-of-Gaussians model (for multimodal distributions). These probability densities are then used to formulate a maximum-likelihood estimation framework for visual search and target detection for automatic object recognition. This learning technique is tested in experiments with modeling and subsequent detection of human faces and non-rigid objects such as hands.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Boolean Comparison of Hardware and Flowcharts (1982)

Boolean comparison is a design verification technique in which two logic networks are compared for functional equivalence using analysis rather than simulation. Boolean comparison was used on the IBM 3081 project to establish that hardware flowcharts and the detailed hardware logic design were functionally equivalent. Hardware flowcharts are a graphic form of a hardware description language which describes the logical behavior of the machine in terms of the inputs, outputs, and latches. The logical correctness of the hardware flowcharts was previously established via cycle simulation. The concepts and techniques of Boolean comparison as used on the IBM 3081 project are described.
LSDA: Large Scale Detection Through Adaptation (2014)

A major challenge in scaling object detection is the difficulty of obtaining labeled images for large numbers of categories. Recently, deep convolutional neural networks (CNNs) have emerged as clear winners on object classification benchmarks, in part due to training with 1.2M+ labeled classification images. Unfortunately, only a small fraction of those labels are available for the detection task. It is much cheaper and easier to collect large quantities of image-level labels from search engines than it is to collect detection data and label it with precise bounding boxes. In this paper, we propose Large Scale Detection through Adaptation (LSDA), an algorithm which learns the difference between the two tasks and transfers this knowledge to classifiers for categories without bounding box annotated data, turning them into detectors. Our method has the potential to enable detection for the tens of thousands of categories that lack bounding box annotations, yet have plenty of classification data. Evaluation on the ImageNet LSVRC-2013 detection challenge demonstrates the efficacy of our approach. This algorithm enables us to produce a &gt;7.6K detector by using available classification data from leaf nodes in the ImageNet tree. We additionally demonstrate how to modify our architecture to produce a fast detector (running at 2fps for the 7.6K detector). Models and software are available at
Deep Speech: Scaling up end-to-end speech recognition (2014)

We are proposing a keyword-based query interface for knowledge bases - including relational or deductive databases - based on contextual background knowledge such as suitable join conditions or synonyms. Join conditions could be extracted from existing referential integrity (foreign key) constaints of the database schema. They could also be learned from other, previous database queries, if the database schema does not contain foreign key constraints. Given a textual representation - a word list - of a query to a relational database, one may parse the list into a structured term. The intelligent and cooperative part of our approach is to hypothesize the semantics of the word list and to find suitable links between the concepts mentioned in the query using contextual knowledge, more precisely join conditions between the database tables. We use a knowledge-based parser based on an extension of Definite Clause Grammars (Dcg) that are interweaved with calls to the database schema to suitably annotate the tokens as table names, table attributes, attribute values or relationships linking tables. Our tool DdQl yields the possible queries in a special domain specific rule language that extends Datalog, from which the user can choose one.
Carotid Baroreceptor Stimulation, Sympathetic Activity, Baroreflex Function, and Blood Pressure in Hypertensive Patients (2010)

In animals, electric field stimulation of carotid baroreceptors elicits a depressor response through sympathetic inhibition. We tested the hypothesis that the stimulation acutely reduces sympathetic vasomotor tone and blood pressure in patients with drug treatment-resistant arterial hypertension. Furthermore, we tested whether the stimulation impairs the physiological baroreflex regulation. We studied 7 men and 5 women (ages 43 to 69 years) with treatment-resistant arterial hypertension. A bilateral electric baroreflex stimulator at the level of the carotid sinus (Rheos) was implanted > or =1 month before the study. We measured intra-arterial blood pressure, heart rate, muscle sympathetic nerve activity (microneurography), cardiac baroreflex sensitivity (cross-spectral analysis and sequence method), sympathetic baroreflex sensitivity (threshold technique), plasma renin, and norepinephrine concentrations. Measurements were performed under resting conditions, with and without electric baroreflex stimulation, for > or =6 minutes during the same experiment. Intra-arterial blood pressure was 193+/-9/94+/-5 mm Hg on medications. Acute electric baroreflex stimulation decreased systolic blood pressure by 32+/-10 mm Hg (range: +7 to -108 mm Hg; P=0.01). The depressor response was correlated with a muscle sympathetic nerve activity reduction (r(2)=0.42; P<0.05). In responders, muscle sympathetic nerve activity decreased sharply when electric stimulation started. Then, muscle sympathetic nerve activity increased but remained below the baseline level throughout the stimulation period. Heart rate decreased 4.5+/-1.5 bpm with stimulation (P<0.05). Plasma renin concentration decreased 20+/-8% (P<0.05). Electric field stimulation of carotid sinus baroreflex afferents acutely decreased arterial blood pressure in hypertensive patients, without negative effects on physiological baroreflex regulation. The depressor response was mediated through sympathetic inhibition.
Expression of Ep-CAM in cervical squamous epithelia correlates with an increased proliferation and the disappearance of markers for terminal differentiation. (1996)

Ep-CAM, an epithelial adhesion molecule, is absent in normal squamous epithelia but can be detected in some squamous carcinomas. Using a panel of monoclonal antibodies to keratinocyte differentiation and proliferation markers, we investigated the association of EP-CAM expression with differentiation-related and/or neoplastic changes in cervical epithelium. Normal endocervical glandular epithelium (Both columnar and reserve cells) appeared strongly positive for EP-CAM, whereas ectocervical squamous epithelial cells did not express this molecule. Expression of Ep-CAM (in basal cells) was sometimes observed in morphologically normal ectocervical tissue but only in areas bordering cervical intraepithelial neoplasia (CIN) lesions. At the early stages of neoplasia the expression of Ep-CAM was regularly present in squamous epithelium, in general consistent with the areas of atypical, undifferentiated cells. Thus, in CIN grades I and II, the basal/suprabasal layers of the epithelia were positive, whereas in CIN grade III lesions, up to 100% of the cells over the whole thickness of the epithelium sometimes excluding the very upper layers, expressed Ep-CAM. A clear increase, not only in number of positive cells but also in levels of Ep-CAM expression (intensity) was observed during progression from CIN I to CIN III. Expression of Ep-CAM in ectocervical lesions did not coincide with a reappearance of the simple epithelium cytokeratins (CK8 and CK18). On the other hand, expression of Ep-CAM in atypical cells of CIN lesions correlated with the disappearance of CK13, which normally marks cells undergoing squamous differentiation. As was shown with Ki-67, a marker for proliferating cell populations, the areas of Ep-CAM expression were also the areas of enhanced proliferation. Cells expressing Ep-CAM did not express involucrin, a marker for cells committed to terminal differentiation. In the majority of both squamous and adenocarcinomas of the cervix a strong expression of Ep-CAM was observed, although some decrease in the expression (both the intensity and the number of positive cells), as compared with CIN III lesions, was observed in the areas of squamous differentiation. This study demonstrates that the expression of Ep-CAM in cervical squamous epithelium is associated with abnormal proliferation of cell populations that are not committed to terminal differentiation.
Pathogen-specific recombinant human polyclonal antibodies: biodefence applications (2004)

The potential use of biological agents such as viruses, bacteria or bacterial toxins as weapons of mass destruction has fuelled significant national and international research and development in novel prophylactic or therapeutic countermeasures. Such measures need to be fast-acting and broadly specific, a hallmark of target-specific polyclonal antibodies (pAbs). As reviewed here, pathogen-specific antibodies in the form of human or animal serum have long been recognised as effective therapies in a number of infectious diseases. This review focuses in particular on the potential biowarfare agents prioritised by the National Institute of Allergy and Infectious Diseases and the Centers for Disease Control and Prevention (CDC), referred to as the category A organisms. Furthermore, it is propose that the last decade of development in recombinant antibody technologies offers the possibility for developing highly specific human monoclonal or polyclonal pathogen-specific antibodies. In particular, pathogen-specific polyclonal human antibodies offer certain advantages over existing hyperimmune serum products, monoclonal antibodies, small molecule drugs and vaccines. Here, the rationale for designing pAb-based therapeutics against the CDC category A microbial agents causing anthrax, botulism, plague, smallpox, tularaemia and viral haemorrhagic fevers, as well as the overall design of such therapeutics, are discussed.
The Cobalt Carbonyl-catalyzed Hydroesterification of Butadiene with Carbon Monoxide and Methanol (1973)

Abstract The cobalt carbonyl-catalyzed hydromethoxycarbonylation of butadiene is studied. The reaction proceeds in the presence of a pyridine base to give methyl 3-pentenoate. Isoquinoline is found to be a better solvent for the production of methyl 3-pentenoate than pyridine, not only because the yield of the ester is somewhat higher in the presence of isoquinoline than in the presence of pyridine, but also because it is far less volatile than pyridine, so that the product can be easily separated from the solvent and the catalyst by distillation. Also, the hydromethoxycarbonylation of methyl 3-pentenoate under somewhat different conditions is found to give a good yield of dimethyl adipate. The hydroformylation of methyl 3-pentenoate is studied as well. The effect of such a solvent as THF, benzene, and acetonitrile in increasing the selectivity to methyl 5-formylpentanoate is found to increase in this order: acetonitrile&amp;lt;benzene&amp;lt;THF.
Strategic impact of broadband telecommunications in insurance, publishing, and health care (1992)

The business environment of the insurance, publishing, and health care industries is described, and key points to their operations where broadband telecommunications can play a role are identified. Broadband applications are analyzed as to whether they provide both an operational and strategic benefit to innovating organizations in each of the three industries. The following impacts of broadband are identified: fast, efficient, and differentiated service to customers, reduced costs, targeted marketing, and market expansion. The traffic characteristics of the applications in terms of bandwidth and business are analyzed and shown to map on to the rollout of North American public network services by first implementing the less bursty, lower bandwidth applications requiring the interconnections of a small number of sites, and later those involving more bursty, higher bandwidth traffic and more numerous sites.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>