gebhart/patrepeval huggingface dataset:
HsS versus a balanced salt solution as a corneal wetting agent during routine cataract extraction and lens implantation (1997)

The HsS was significantly more effective than a balanced salt solution in maintaining corneal moisture, clarity, and transparency. The use of HsS may be a safer, more effective option in ophthalmic surgery because it minimizes the obstructed visualization of the surgical field caused by frequent corneal irrigation and loss of surgeon concentration.
The design of a GUI paradigm based on tablets, two-hands, and transparency (1997)

Article The design of a GUI paradigm based on tablets, two-hands, and transparency Share on Authors: Gordon Kurtenbach Alias I Wavefront, 110 Richmond Street East, Toronto, Canada, M5C 1P1 Alias I Wavefront, 110 Richmond Street East, Toronto, Canada, M5C 1P1View Profile , George Fitzmaurice Alias I Wavefront, 110 Richmond Street East, Toronto, Canada, M5C 1P1 Alias I Wavefront, 110 Richmond Street East, Toronto, Canada, M5C 1P1View Profile , Thomas Baudel Alias I Wavefront, 110 Richmond Street East, Toronto, Canada, M5C 1P1 Alias I Wavefront, 110 Richmond Street East, Toronto, Canada, M5C 1P1View Profile , Bill Buxton Alias I Wavefront, 110 Richmond Street East, Toronto, Canada, M5C 1P1 Alias I Wavefront, 110 Richmond Street East, Toronto, Canada, M5C 1P1View Profile Authors Info & Claims CHI '97: Proceedings of the ACM SIGCHI Conference on Human factors in computing systemsMarch 1997 Pages 35–42https://doi.org/10.1145/258549.258574Published:27 March 1997 104citation1,759DownloadsMetricsTotal Citations104Total Downloads1,759Last 12 Months33Last 6 weeks3 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Novel chitosan derivative nanoparticles enhance the immunogenicity of a DNA vaccine encoding hepatitis B virus core antigen in mice (2007)

This study demonstrated the potential of the novel chitosan derivative nanoparticles for safe and effective DNA vaccine delivery.
Ferric carboxymaltose reduces the number of red blood cell units transfused and allows transfusion independence to be obtained in patients with iron deficiency anemia secondary to gastrointestinal chronic blood loss (2016)

In patients with IDA secondary to CBL, FCM significantly reduces the need of transfusions and achieves transfusion independence in half of the cases.
Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate (1981)

Isolates of Escherichia coli from human urinary tract infections frequently express adherence properties found less often among normal intestinal isolates. These properties include adherence to human uroepithelial cells and primary monkey kidney cells, as well as D-mannose-resistant hemagglutination of human erythrocytes, and they are mediated by a pilus type different from type 1. The genes encoding this pilus type (pyelonephritis-associated pili, pap) and those encoding type 1 pili have been cloned from a urinary tract infection isolate of E. coli and transferred to an E. coli K-12 derivative. The recombinant plasmids were found to express functional pili and to endow the new host with all of the adherence properties of the urinary tract infection isolate. Both pilus types were found to be genetically distinct, and unlike the adherence genes from bovine, porcine, and human diarrheal isolates, both were found to be chromosomally encoded.
Characterization of the myosin light-chain-2 gene of Drosophila melanogaster. (1985)

Recombinant DNA clones encoding the Drosophila melanogaster homolog of the vertebrate myosin light-chain-2 (MLC-2) gene have been isolated. This single-copy gene maps to the chromosomal locus 99E. The nucleotide sequence was determined for a 3.4-kilobase genomic fragment containing the gene and for two MLC-2 cDNA clones generated from late pupal mRNA. Comparison of these sequences shows that the gene contains two introns, the positions of which are conserved in the corresponding rat sequence. Extension of a primer homologous to the mRNA reveals two start sites for transcription 12 nucleotides apart. The sequence TATA is not present ahead of the mRNA cap site. There are two major sites of poly(A) addition separated by 356 nucleotides. The protein sequence derived from translation of the cDNA sequence shows a high degree of homology with that for the DTNB myosin light chain (MLC-2) of chicken. A lower degree of sequence homology was seen in comparisons with other evolutionarily related calcium-binding proteins. RNA blots show high levels of expression of several transcripts during the developmental time stages when muscle is being produced. In vitro translation of hybrid-selected RNA produces two polypeptides which comigrate on two-dimensional gels with proteins from Drosophila actomyosin, although the cDNA sequence reveals only one 26-kilodalton primary translation product.
The development of atypical haemolytic-uraemic syndrome is influenced by susceptibility factors in factor H and membrane cofactor protein: evidence from two independent cohorts (2005)

This study suggests that there are naturally occurring susceptibility factors in CFH and MCP for the development of atypical HUS.
Decline in Microbial Studies for Patients with Pulmonary Infections (2004)

There appears to be a substantial decline in the quality of microbiology done in the context of pulmonary infections during the current era, compared with 3 decades ago, and the question posed to the medical community is whether this change is acceptable or reversible. The decline seems to be well documented. In the prepenicillin era, for example, Bullowa [1] reported his own experience with 14000 cases of pneumonia, from 80% of which S. pneumoniae was recovered. In the 1950s, most reports had a yield of pneumococci in patients hospitalized with community-acquired pneumonia of 40%– 70%. During the past 15 years, it has been a challenge to find any US-based study in which the yield of pneumococci was 118%. The experience at Johns Hopkins
Audio-visual speech modeling for continuous speech recognition (2000)

This paper describes a speech recognition system that uses both acoustic and visual speech information to improve recognition performance in noisy environments. The system consists of three components: a visual module; an acoustic module; and a sensor fusion module. The visual module locates and tracks the lip movements of a given speaker and extracts relevant speech features. This task is performed with an appearance-based lip model that is learned from example images. Visual speech features are represented by contour information of the lips and grey-level information of the mouth area. The acoustic module extracts noise-robust features from the audio signal. Finally the sensor fusion module is responsible for the joint temporal modeling of the acoustic and visual feature streams and is realized using multistream hidden Markov models (HMMs). The multistream method allows the definition of different temporal topologies and levels of stream integration and hence enables the modeling of temporal dependencies more accurately than traditional approaches. We present two different methods to learn the asynchrony between the two modalities and how to incorporate them in the multistream models. The superior performance for the proposed system is demonstrated on a large multispeaker database of continuously spoken digits. On a recognition task at 15 dB acoustic signal-to-noise ratio (SNR), acoustic perceptual linear prediction (PLP) features lead to 56% error rate, noise robust RASTA-PLP (relative spectra) acoustic features to 7.2% error rate and combined noise robust acoustic features and visual features to 2.5% error rate.
Fibroblast Growth Factor 2-Retargeted Adenoviral Vectors Exhibit a Modified Biolocalization Pattern and Display Reduced Toxicity Relative to Native Adenoviral Vectors (2000)

Targeted vectors provide a number of advantages for systemic and local gene delivery strategies. Several groups have investigated the utility of using various ligands to alter the tropism of adenovirus (Ad) vectors. We have previously demonstrated that fibroblast growth factor (FGF) ligands can specifically target DNA transfection and Ad transduction through high-affinity FGF receptors (FGFRs). FGFRs are overexpressed in abnormally proliferating tissues, such as malignancies. The present studies explore the effects of retargeting with FGF2 on the tissue localization pattern and the systemic toxicity of Ad in mice. Results of semiquantitative PCR analyses indicate that the distribution of FGF2-Ad vector genome sequences after intravenous administration in mice is altered. Markedly lower amounts (10- to 20-fold) of FGF2-Ad localize to the liver when compared with native Ad. This decrease in liver deposition translates into a significant reduction in subsequent toxicity as measured by serum transaminases and histopathology in mice injected with FGF2-AdHSV-thymidine kinase with and without ganciclovir administration. In an intraperitoneal model of ovarian cancer, FGF2-Ad generates increased transgene expression in tumor tissue when compared with Ad. Taken together, these results indicate that the retargeting of Ad with FGF2 results in a more efficient vector system for systemic and regional gene therapy applications, with concomitant lower levels of systemic toxicity.
Synthesis of I‐Hydroxy‐2,4‐diphenylpyrrolo[2,3‐d] pyridazin‐7(6H)one (1979)

Abstract The synthesis of 1‐hydroxy‐2,4‐diphenylpyrrolo[2,3‐ d ]pyridazin‐7(6 H )one is described.
Hair coloring and waving using oxidases (1991)

For hair coloring and waving, biochemical oxidation using enzymes such as uricase, glucose oxidase, galactose oxidase, laccase, and tyrosinase was investigated in commercial formulations instead of the usual chemical oxidations. Coloring of goat hair was satisfactorily accomplished in the aromatic amine precursor system using uricase and glucose oxidase. While galactose oxidase and tyrosinase showed a slight coloring, laccase did not lead to any coloring. Enzymatic hair waving was evaluated according to the Kirby method. The results ofwaviffg efficiency and wave retention ratio showed that the waving effect with uricase neutralization is almost equal to that with sodium bromide.
Induction of TCR Vβ-Specific CD8+ CTLs by TCR Vβ-Derived Peptides Bound to HLA-E (2001)

Previous studies have identified murine and human regulatory CD8+ T cells specific for TCR-Vbeta families expressed on autologous activated CD4+ T cells. In the mouse, these regulatory CD8+ T cells were shown to be restricted by the MHC class Ib molecule, Qa-1. In the present study, we asked whether HLA-E, the human functional equivalent of Qa-1, binds Vbeta peptides and whether the HLA-E/Vbeta-peptide complex induces and restricts human CD8+ CTLs. We first created stable HLA-E gene transfectants of the C1R cell line (C1R-E). Two putative HLA-E binding nonapeptides identified in human TCR Vbeta1 and Vbeta2 chains (SLELGDSAL and LLLGPGSGL, respectively) were shown to bind to HLA-E. CD8+ T cells could be primed in vitro by C1R-E cells loaded with the Vbeta1 (C1R-E/V1) or Vbeta2 (C1R-E/V2) peptide to preferentially kill C1R-E cells loaded with the respective inducing Vbeta peptide, compared with targets loaded with the other peptides. Priming CD8+ T cells with untreated C1R-E cells did not induce Vbeta-specific CTLs. Of perhaps more physiological relevance was the finding that the CD8+ CTLs primed by C1R-E/V1 also preferentially killed activated autologous TCR Vbeta1+. Similar results were observed in reciprocal experiments using C1R-E/V2 for priming. Furthermore, anti-CD8 and anti-MHC class I mAbs inhibited this Vbeta-specific killing of C1R-E and CD4+ T cell targets. Taken together, the data provide evidence that certain TCR-Vbeta peptides can be presented by HLA-E to further induce Vbeta-specific CD8+ CTLs.
Leukotriene B 4 triggers release of the cathelicidin LL‐37 from human neutrophils: novel lipid‐peptide interactions in innate immune responses (2007)

In humans, the antimicrobial peptide LL-37 and the potent chemotactic lipid leukotriene B4 (LTB4) are important mediators of innate immunity and host defense. Here we show that LTB4, at very low (1 nM) concentrations, strongly promotes release of LL-37 peptides from human neutrophils (PMNs) in a time- and dose-dependent manner, as determined by Western blot, enzyme-linked immunoassay (ELISA), and antibacterial activity. The LTB4-induced LL-37 release is mediated by the BLT1 receptor, and protein phosphatase-1 (PP-1) inhibits the release by suppressing the BLT1-mediated exocytosis of PMN granules. Conversely, LL-37 elicits translocation of 5-lipoxygenase (5-LO) from the cytosol to the perinuclear membrane in PMNs and promotes the synthesis and release of LTB4, particularly from cells primed with LPS or GM-CSF. Furthermore, LL-37 stimulates PMN phagocytosis of Escherichia coli particles, a functional response that is enhanced by LTB4, especially in GM-CSF pretreated cells. In these cells, LL-37 also enhances LTB4-induced phagocytosis. Hence, in human PMNs, positive feedback circuits exist between LL-37 and LTB4 that reciprocally stimulate the release of these mediators with the potential for synergistic bioactions and enhanced immune responses. Moreover, these novel lipid-peptide signaling pathways may offer new opportunities for pharmacological intervention and treatment of chronic inflammatory diseases.
How to Generate Cyclically Permutable Codes From Cyclic Codes (2006)

On the basis of the characteristics of cyclic codes, the codeword space can be partitioned into small subspaces where cyclically shifted codewords of a particular codeword occupy the same subspace. A cyclically permutable code generates codewords belonging to each subspace. However, no approach for the efficient construction of cyclically permutable code from binary cyclic codes has been proposed thus far. In this study, we propose an approach for the efficient and systematic construction of a cyclically permutable code from a cyclic code by utilizing an algebraic property. The proposed coding method improves the robustness of watermarking, particularly for video frames, against a clipping attack
Molecular Cloning and Functional Expression of Human Parathyroid Calcium Receptor cDNAs (1995)

Parathyroid cells express a cell surface receptor, coupled to the mobilization of intracellular Ca2+, that is activated by increases in the concentration of extracellular Ca2+ and by a variety of other cations. This "Ca2+ receptor" (CaR) serves as the primary physiological regulator of parathyroid hormone secretion. Alterations in the CaR have been proposed to underlie the increases in Ca2+ set-point seen in primary hyperparathyroidism due to parathyroid adenoma. We have isolated human CaR cDNAs from an adenomatous parathyroid gland. The cloned receptor, expressed in Xenopus oocytes, responds to extracellular application of physiologically relevant concentrations of Ca2+ and other CaR agonists. The rank order of potency of CaR agonists displayed by the native receptor (Gd3+ > neomycin B > Ca2+ > Mg2+) is maintained by the expressed receptor. The nucleotide sequence of the human CaR cDNA predicts a protein of 1078 amino acids with high sequence similarity to a bovine CaR, and displays seven putative membrane-spanning regions common to G protein-coupled receptors. The deduced protein sequence shows potential sites for N-linked glycosylation and phosphorylation by protein kinase C and has a low level of sequence similarity to the metabotropic glutamate receptors. Comparison of the cDNA sequence to that of the normal human CaR gene showed no alteration in the coding region sequence of the CaR in this particular instance of parathyroid adenoma. Human cDNA clones with differing 5'-untranslated regions were isolated, suggesting alternative splicing of the parathyroid CaR mRNA. A rare variant cDNA clone representing a 10 amino acid insertion into the extracellular domain was also isolated. Northern blot analysis of normal and adenomatous parathyroid gland mRNA identified a predominant transcript of approximately 5.4 kilobases, and less abundant transcripts of approximately 10, 4.8 and 4.2 kilobases in RNA from the adenoma. While there is no evidence for alteration of the primary amino acid sequence of the CaR in this adenoma, modulation of CaR biosynthesis through alternative RNA processing may play a role in set-point alterations.
Pituitary homeobox 2 (PITX2) protects renal cancer cell lines against doxorubicin toxicity by transcriptional activation of the multidrug transporter ABCB1 (2013)

The multidrug resistance (MDR) P-glycoprotein ABCB1 plays a major role in MDR of malignant cells and is regulated by various transcription factors, including Wnt/β-catenin/TCF4. The transcription factor PITX2 (Pituitary homeobox-2) is essential for embryonic development. PITX2 operates by recruiting and interacting with β-catenin to increase the expression of growth-regulating genes, such as cyclin D1/2 and c-Myc. The importance of PITX2 in malignancy is not yet known. Here we demonstrate that in the renal cancer cell lines ACHN and A498, the level of ABCB1 expression and function correlate with nuclear PITX2 localization and PITX2-luciferase reporter gene activity (A498 > ACHN). In A498 cells, doxorubicin toxicity is augmented by the ABCB1 inhibitor, PSC833. PITX2 overexpression increases ABCB1 expression and cell survival in ACHN cells. Silencing of PITX2 by siRNA downregulates ABCB1 and induces a greater chemotherapeutic response to doxorubicin in A498 cells, as determined by MTT cell viability and clonogenic survival assays. Two PITX2 binding sequences were identified in the ABCB1 promoter sequence. PITX2 binding was confirmed by chromatin immunoprecipitation. β-Catenin is not required for PITX2 upregulation of ABCB1 because ABCB1 mRNA increased and doxorubicin toxicity decreased upon PITX2 overexpression in β-catenin(-/-) cells. The data show for the first time that ABCB1 is a target gene of PITX2 transcriptional activity, promoting MDR and cell survival of cancer cells.
CMOS compatible fully integrated Mach-Zehnder interferometer in SOI technology (2000)

We present a fully integrated Mach-Zehnder interferometer in silicon-on-insulator technology. Modulation of the index of refraction is achieved through the plasma dispersion effect resulting in a bandwidth in the 10 MHz range. A particular and innovative design makes this device completely compatible with CMOS technology allowing electronic functions to be integrated on the same substrate. Measurement results, limitations due to thermooptic effect and absorption related to charge injection together with further improvements are discussed.
Design and Evaluation of a Reconfigurable Antenna Array for MIMO Systems (2008)

New reconfigurable antenna array is demonstrated for multiple input multiple output (MIMO) communication systems that improves link capacity in closely spaced antenna arrays. The antenna system consists of an array of two printed dipoles separated by a distance of a quarter wavelength. Each of the dipoles can be reconfigured in length using PIN diode switches. The switch configuration can be modified in a manner adaptive to changes in the environment. The configuration of switches effects the mutual coupling between the array elements, and subsequently, the radiation pattern of each antenna, leading to different degrees of pattern diversity which can be used to improve link capacity. The PIN diode-based reconfigurable antenna solution is first motivated through a capacity analysis of the antenna in a clustered MIMO channel model. A new definition of spatial correlation coefficient is introduced to include the effects of antenna mismatch and radiation efficiency when quantifying the benefit of pattern diversity. Next, the widespread applicability of the proposed technique is demonstrated, relative to conventional half wavelength printed dipoles, using computational electromagnetic simulation in an outdoor and indoor environment and field measurements in an indoor laboratory environment. It is shown for the 2 times 2 system considered in this paper, that an average improvement of 10% and 8% is achieved in link capacity for a signal to noise ratio (SNR) respectively of 10 dB and 20 dB in an indoor environment compared to a system employing non reconfigurable antenna arrays.
Review of quantitative phase-digital holographic microscopy: promising novel imaging technique to resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders (2014)

Quantitative phase microscopy (QPM) has recently emerged as a new powerful quantitative imaging technique well suited to noninvasively explore a transparent specimen with a nanometric axial sensitivity. In this review, we expose the recent developments of quantitative phase-digital holographic microscopy (QP-DHM). Quantitative phase-digital holographic microscopy (QP-DHM) represents an important and efficient quantitative phase method to explore cell structure and dynamics. In a second part, the most relevant QPM applications in the field of cell biology are summarized. A particular emphasis is placed on the original biological information, which can be derived from the quantitative phase signal. In a third part, recent applications obtained, with QP-DHM in the field of cellular neuroscience, namely the possibility to optically resolve neuronal network activity and spine dynamics, are presented. Furthermore, potential applications of QPM related to psychiatry through the identification of new and original cell biomarkers that, when combined with a range of other biomarkers, could significantly contribute to the determination of high risk developmental trajectories for psychiatric disorders, are discussed.
Differential P1-purinergic modulation of human Schlemm's canal inner-wall cells (2005)

Intraocular pressure is directly dependent on aqueous humor flow into, and resistance to flow out of, the eye. Adenosine has complex effects on intraocular pressure. Stimulation of A1 and A2A adenosine receptors changes intraocular pressure oppositely, likely through opposing actions on the outflow of aqueous humor. While the cellular sites regulating outflow resistance are unknown, the cells lining the inner wall of Schlemm's canal (SC) are a likely regulatory site. We applied selective adenosine receptor agonists to SC cells in vitro to compare the responses to A1 and A2A stimulation. Parallel studies were conducted with human inner-wall SC cells isolated by a novel enzyme-assisted technique and with cannula-derived mixed inner- and outer-wall SC cells. A1 agonists increased whole cell currents of both inner-wall and cannula-derived SC cells. An A2A agonist reduced currents most consistently in specifically inner-wall SC cells. Those currents were also increased by A2B, but not consistently affected by A3, stimulation. A1, A2A, and A3 agonists all increased SC-cell intracellular Ca2+. The electrophysiological results are consistent with the possibility that inner-wall SC cells may mediate the previously reported modulatory effects of adenosine on outflow resistance. The results are also consistent with the presence of functional A2B, as well as A1, A2A, and A3 adenosine receptors in SC cells.