gebhart/patrepeval huggingface dataset:
A regulatory role for the ABI3 gene in the establishment of embryo maturation in Arabidopsis thaliana (1995)

ABSTRACT The ABI3 gene product of Arabidopsis is essential for correct completion of seed maturation. A severe mutant allele at this locus results in seed that remain green, fail to establish desiccation tolerance, and that germinate at a developmental stage when wild-type seed will not. Moreover, the formation of leaf primordia and xylem differentiation, both characteristic of germinating wildtype seedlings, can be observed in embryos harvested 12 days after flowering. Thus, mature abi3 embryos reach a developmental state that more closely resembles the character of a developing seedling rather than that of a dormant embryo. Previous studies of this gene have resulted in the suggestion that ABI3 is a transducer of abscisic acid induced seed dormancy. Our results demonstrate that the ABI3 gene product can be most accurately described as one of the major regulators of the transition between embryo maturation and early seedling development, rather than simply a transducer of the abscisic acid signal.
异于去是，或在是其之所是之外（续） (2007)

但与此先于原初的这个关系不还是一种收回（recuperation）吗？让我们将此更仔细地察看一下。应承者之应并不把这不同时话题化，就好像这不同时是被留下来的、被记住的或被历史地重构出来的一样。此应不能进行话题化或进行把握。但这不是出于软弱无力；对不能被容纳者而言就无所谓容纳能力。此一非现在之为不可把握是由于其无比巨大，
Diels—Alder Cycloaddition as an Efficient Tool for Linking π‐Donors onto Fullerene C60 (2007)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Electro-optic bistability in a LiNbO 3 microdisk resonator (2008)

The authors experimentally demonstrate feedback-controlled electro-optic bistability in the whispering gallery travelling-wave optical resonance of a LiNbO3 microdisk. Simulations confirm the physical origin of the behaviour.
Blending Cultural Business Styles (2002)

ADR and CS obtain good clinical results for symptomatic single-level cervical DDD. ADR demonstrated a significant advantage over CS in the improvement of VAS arm and reduced progression of ALOD of the adjacent lower disc. No statistically significant difference of dysphonia or dysphagia between the two groups were seen, attributed to their comparable zero profile.
Sequential expression of protooncogenes during lectin-stimulated mitogenesis of normal human lymphocytes. (1986)

The proliferation of non-neoplastic T lymphocytes is regulated, in part, by the coordinated expression of genes encoding T-cell growth factor (interleukin 2, IL2), IL2 receptors (IL2R), and transferrin receptors (TFR). In addition to growth factors and their receptors, protooncogenes may regulate lymphocyte proliferation. We used cloned cDNAs homologous to 21 different protooncogenes to screen for their expression at the mRNA level in human peripheral blood mononuclear cells (PBMC) stimulated with the mitogenic lectin phytohemagglutinin (PHA), and we compared the time course of accumulation of mRNAs for these protooncogenes to that of mRNAs for the IL2, IL2R, TFR, and histone H3 genes. mRNAs for c-abl, c-ets, c-yes, and N-ras were present in unstimulated PBMC. After stimulation of PBMC by PHA, we detected marked increases within 10 min in the levels of mRNA for c-fos and c-myc; within 6 hr for IL2 and IL2R mRNAs; within 14 hr for c-myb, p53, N-ras, and TFR mRNAs; and within 24-36 hr for H3 mRNA. Expression of c-abl, c-ets, and c-yes increased gradually following stimulation with PHA. None of the other protooncogenes tested was expressed in PBMC. Addition of the protein synthesis inhibitor cycloheximide, before the addition of PHA to cultures, abolished the PHA-induced accumulation of mRNAs for c-myb, N-ras, and TFR, but not of mRNAs for c-fos, c-myc, IL2, and IL2R. These data indicate that c-fos, c-myc, IL2, and IL2R belong to a group of genes expressed early, whereas c-myb, N-ras, and TFR belong to a group of genes expressed later in PHA-activated PBMC, and that the products of the c-fos and c-myc protooncogenes are not required for expression of IL2 or IL2R genes. Addition of purified IL2 augmented the expression of the later-expressed genes c-myb, p53, N-ras, and TFR in PHA-stimulated cultures of PBMC, as well as of the early genes c-myc and IL2R, but not of c-fos and IL2, thus suggesting that PHA and IL2 stimulate the expression of overlapping, but nonidentical, sets of genes in PBMC.
The Epidemiology of Pseudomonas aeruginosa in Oncology Patients in a General Hospital (1989)

Pseudomonas aeruginosa colonization and infection was studied over a 6-mo period in a 36-bed mixed general medical-oncology unit. We used selective media for serial surveillance cultures on 283 patients, the environment, and personnel. Twelve percent of patients were colonized on admission and 10% acquired P. aeruginosa. Using serotyping and multilocus enzyme electrophoresis, we identified 63 genetically distinctive strains; four prevalent strains accounted for 21% of isolates. Only 5 of 33 nosocomial acquisitions were due to horizontal transmission. Nine acquisitions were linked to environmental sources (e.g., sink surfaces), which often harbored antibiotic-resistant strains but posed a risk only to oncology patients. Although significant Pseudomonas infections occurred in only 11% of colonized patients, 63% of colonized severely neutropenic patients--predominantly those who had acquired the prevalent, often environmentally linked strains--developed infections. Thus, P. aeruginosa was a significant pathogen in oncology patients; typing by multilocus enzyme electrophoresis allowed the detection of important environmental sources.
An Upstream Element Containing an ETS Binding Site Is Crucial for Transcription of the Human Presenilin-1 Gene (1999)

Deletion mapping of the human presenilin-1 (PS1) promoter delineated the most active fragment from -118 to +178 in relation to the transcription start site mapped in this study, in both human neuroblastoma SK-N-SH and hepatoma HepG2 cells. 5' deletions revealed that a crucial element controlling over 90% of the promoter activity in these cell lines is located between -22 and -6. A mutation altering only two nucleotides of the ETS consensus sequence present at -12 (GGAA to TTAA) has a similar effect. Electrophoretic mobility shift assays showed that a set of specific complexes between nuclear factors and the PS1 promoter are eliminated by this point mutation, as well as by competition with an ETS consensus oligonucleotide. Competition experiments in DNase I footprinting correlated with electrophoretic mobility shift assays and showed that only one of several footprints over the PS1 promoter is eliminated by competition with an ETS consensus oligonucleotide. It extends from -14 to -6 and surrounds the ETS motif present at -12. Thus, a crucial ETS element is present at -12 and binds a protein(s) recognizing specifically the ETS consensus motif. At least one such complex is eliminated by preincubating the nuclear extract with an antibody with broad cross-reactivity with Ets-1 and Ets-2 proteins, thus confirming that an ETS transcription factor(s) recognizes the -12 motif. Several Sp1 binding motifs at positions -70, -55, and +20 surround this ETS element. Competition DNase I footprinting showed that Sp1-like nuclear factors recognize specifically these sites in both cell lines. Furthermore, a combination of 5' and 3' deletions indicated the presence of positive promoter elements between -96 and -35 as well as between +6 and +42. Thus, transfection and footprinting assays correlate to suggest that Sp1 transcription factor(s) bind at several sites upstream and downstream from the initiation site and activate the transcription of the PS1 promoter. Sequences downstream from the transcription initiation site also contain major control elements. 3' deletions from +178 to +107 decreased promoter activity by 80%. However, further deletion to +42 increased promoter activity by 3-4-fold. Collectively, these data indicate that sequences upstream and downstream from the transcription start site each control over 80% of the promoter activity. Hence, this suggests that protein-protein interactions between factors recognizing downstream and upstream sequences are involved.
Dominant Negative Mutants of Transducin-α That Block Activated Receptor (2006)

Mutations counterpart to dominant negative RasSer17Asn in the alpha-subunits of heterotrimeric G-proteins are known to also produce dominant negative effects. The mechanism of these mutations remains poorly understood. Here, we examined the effects and mechanism of the Ser43Cys and Ser43Asn mutants of transducin-like chimeric Gtalpha* in the visual signaling system. Our analysis showed that both mutants have reduced affinity for GDP and are likely to exist in an empty-or partially occupied-pocket state. S43C and S43N retained the ability to interact with Gtbetagamma and, as heterotrimeric proteins, bind to photoexcited rhodopsin (R*). The interaction with R* is unproductive as the mutants failed to bind GTPgammaS and become activated. S43C and S43N inhibited R*-dependent activation of Gtalpha* and Gtalpha, apparently by blocking R*. Finally, both Gtalpha* mutants lacked interaction with the gamma-subunit of PDE6, an effector protein in phototransduction. These results indicate that the S43C and S43N mutants of Gtalpha* are dominant negative inhibitors that bind and block the activated receptor in a mechanism that parallels that of RasSer17Asn. Dominant negative mutants of Gtalpha sequestering R*, such as S43C and S43N, may become useful instruments in probing the mechanisms of visual dysfunctions caused by abnormal phototransduction signaling.
云端使者 马努特·博尔 (2006)

姚明和奥尼尔哪怕取得再大的成就,在马努特·博尔的身高面前,他们也得自叹弗如,因为2．31米的博尔是 NBA历史上最高的球员,但一次可怕的交通事故,使得博尔目前在身体上和财政上都陷入了难以为继的窘境中。
Insertion of an N-Terminal 6-Aminohexanoic Acid after the 7 Amino Acid Position of Glucagon-Like Peptide-1 Produces a Long-Acting Hypoglycemic Agent (2001)

The use of glucagon-like peptide-1 (GLP-1) as a routine treatment for type 2 diabetes mellitus is undermined by its short biological half-life. A cause of degradation is its cleavage at the N-terminal HAE sequence by the enzyme dipeptidyl peptidase IV (DPP IV). To protect from DPP IV, we have studied the biological activity of a GLP-1 analog in which 6-aminohexanoic acid (Aha) is inserted between histidine and alanine at positions 7 and 8. We have compared the biological activity of this new compound, GLP-1 Aha8, with the previously described GLP-1 8-glycine (GLP-1 Gly8) analog. GLP-1 Aha8 (10 nm) was equipotent with GLP-1 (10 nm) in stimulating insulin secretion in RIN 1046-38 cells. As with GLP-1 Gly8, the binding affinity of GLP-1 Aha8 for the GLP-1 receptor in intact Chinese hamster ovary (CHO) cells expressing the human GLP-1 receptor (CHO/GLP-1R cells) was reduced (IC50: GLP-1, 3.7± 0.2 nm; GLP-1 Gly8, 41 ± 9 nm; GLP-1 Aha8, 22 ± 7 nm). GLP-1 Aha8 was also shown to stimulate intracellular cAMP production 4-fold above basal at concentrations as low as 0.5 nm. However, it exhibited a higher ED50 when compared to GLP-1 and GLP-1 Gly8 (ED50: GLP-1, 0.036 ± 0.002 nm, GLP-1 Gly8, 0.13 ± 0.02 nm, GLP-1 Aha8, 0.58 ± 0.03 nm). A series of d-amino acid-substituted GLP-1 compounds were also examined to assess the importance of putative peptidase-sensitive cleavage sites present in the GLP-1 molecule. They had poor binding affinity for the GLP-1 receptor, and none of these compounds stimulated the production of intracellular cAMP in CHO/GLP-1R cells or insulin secretion in RIN 1046-38 cells. GLP-1 Aha8 (24 nmol/kg) administered sc to fasted Zucker (fa/fa) rats (mean blood glucose, 195 ± 32 mg/dl) lowered blood glucose levels to a nadir of 109 ± 3 mg/dl, and it remained significantly lower for 8 h. Matrix-assisted linear desorption ionization-time of flight mass spectrometry of GLP-1 Aha8 incubated with DPP IV (37 C, 2 h) did not exhibit an N-terminal degradation product. Taken together, these results show that insertion of Aha after the 7 position in GLP-1 produces an effective, long-acting GLP-1 analog, which may be useful in the treatment of type 2 diabetes mellitus.
Development of linear and nonlinear predictive QSAR models and their external validation using molecular similarity principle for anti-HIV indolyl aryl sulfones (2008)

Quantitative structure-activity relationship (QSAR) studies have been carried out on indolyl aryl sulfones, a class of novel HIV-1 non-nucleoside reverse transcriptase inhibitors, using physicochemical, topological and structural parameters along with appropriate indicator variables. The statistical tools used were linear methods (e.g., stepwise regression analysis, partial least squares (PLS), factor analysis followed by multiple regression (FA-MLR), genetic function approximation combined with multiple linear regression (GFA-MLR) and GFA followed by PLS or G/PLS and nonlinear method (artificial neural network or ANN). In case of physicochemical parameters, GFA-MLR generated the best Equation (n = 97, R(2) = 0.862, Q(2) = 0.821). Using topological parameters, the best Equation (based on leave-one-out Q(2)) was obtained with stepwise regression technique (n = 97, R(2) = 0.867, Q(2) = 0.811). When topological and physicochemical parameters were used in combination, statistical quality increased to a great extent (n = 97, R(2) = 0.891, Q(2) = 0.849 from stepwise regression). Furthermore, the whole dataset had been divided into test (25% of whole dataset) and training (remaining 75%) sets. Models were developed based on the training set and predictive potential of such models was checked from the test set. The selection of the training set was based on K-means clustering of the standardized descriptors (topological and physicochemical). In this case also the best results were obtained with stepwise regression (n = 72, R(2) = 0.906, Q(2) = 0.853) but external predictive capacity of this model ([image omitted] ) was inferior to the model developed from GFA-MLR technique (R(2) = 0.883, Q(2) = 0.823, [image omitted] ). However, the squared regression coefficient between observed activity and predicted activity values of the test set compounds for the best linear model, i.e., GFA-MLR (r(2) = 0.736) was lower in comparison to the best nonlinear model developed using artificial neural network (r(2) = 0.781). Thus, based on external validation, the ANN models were superior to the linear models. The predictive potential of the best linear Equation (stepwise regression model) was superior to that of the previously published CoMFA (Q(2) = 0.81, SDEP(Test) = 0.89) on the same data set (Ragno R. et al., J Med Chem 2006, 49, 3172-3184). Furthermore, the physicochemical parameter based models also supported the previous observations based on docking (Ragno R. et al., J Med Chem 2005, 48, 213-223).
Functional and structural studies of a small F-actin binding domain (1992)

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1992.
Sketch-sketch revolution (2011)

We describe Sketch-Sketch Revolution, a new tutorial system that allows any user to experience the success of drawing content previously created by an expert artist. Sketch-Sketch Revolution not only guides users through the application user interface, it also provides assistance with the actual sketching. In addition, the system offers an authoring tool that enables artists to create content and then automatically generates a tutorial from their recorded workflow history. Sketch-Sketch Revolution is a unique hybrid tutorial system that combines in-product, content-centric and reactive tutorial methods to provide an engaging learning experience. A qualitative user study showed that our system successfully taught users how to interact with a drawing application user interface, gave users confidence they could recreate expert content, and was uniformly considered useful and easy to use.
Carbon Formation from Carbon Monoxide-Hydrogen Mixtures over Iron Catalysts.I. Properties of Carbon Formed (1959)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbon Formation from Carbon Monoxide-Hydrogen Mixtures over Iron Catalysts.I. Properties of Carbon FormedP. L. Walker Jr., J. F. Rakszawski, and G. R. ImperialCite this: J. Phys. Chem. 1959, 63, 2, 133–140Publication Date (Print):February 1, 1959Publication History Published online1 May 2002Published inissue 1 February 1959https://pubs.acs.org/doi/10.1021/j150572a002https://doi.org/10.1021/j150572a002research-articleACS PublicationsRequest reuse permissionsArticle Views790Altmetric-Citations110LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Stem Cells for the Treatment of Urinary Incontinence (2010)

Stress urinary incontinence (SUI) is highly prevalent. As of now, there is no minimally invasive long-term treatment available. Adult stem cells are nonimmunogenic and have the ability to self-renew and to differentiate into multiple cell types. Over the past decade, in vivo studies have described periurethral injections of adult-derived stem cells for the treatment of SUI. The ultimate goal has been to achieve a permanent cure for SUI by restoration of the intrinsic and extrinsic urethral sphincter and the surrounding connective tissue, including peripheral nerves and blood vessels. For this purpose, future studies need to focus on delivery systems, cell survival, and functional improvement of the urethral closure mechanism, including improvement of innervation and vascularization.
Divergent Synthesis of 48 Heparan Sulfate-Based Disaccharides and Probing the Specific Sugar–Fibroblast Growth Factor-1 Interaction (2012)

Several biological processes involve glycans, yet understanding their ligand specificities is impeded by their inherent diversity and difficult acquisition. Generating broad synthetic sugar libraries for bioevaluations is a powerful tool in unraveling glycan structural information. In the case of the widely distributed heparan sulfate (HS), however, the 48 theoretical possibilities for its repeating disaccharide call for synthetic approaches that should minimize the effort in an undoubtedly huge undertaking. Here we employed a divergent strategy to afford all 48 HS-based disaccharides from just two orthogonally protected disaccharide precursors. Different combinations and sequence of transformation steps were applied with many downstream intermediates leading up to multiple target products. With the full disaccharide library in hand, affinity screening with fibroblast growth factor-1 (FGF-1) revealed that four of the synthetic sugars bind to FGF-1. The molecular details of the interaction were further clarified through X-ray analysis of the sugar-protein cocrystals. The capability of comprehensive sugar libraries in providing key insights in glycan-ligand interaction is, thus, highlighted.
Avidin is a slow-binding inhibitor of pyruvate carboxylase (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAvidin is a slow-binding inhibitor of pyruvate carboxylaseRonald G. Duggleby, Paul V. Attwood, John C. Wallace, and D. Bruce KeechCite this: Biochemistry 1982, 21, 14, 3364–3370Publication Date (Print):July 1, 1982Publication History Published online1 May 2002Published inissue 1 July 1982https://pubs.acs.org/doi/10.1021/bi00257a018https://doi.org/10.1021/bi00257a018research-articleACS PublicationsRequest reuse permissionsArticle Views393Altmetric-Citations54LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Skin Closure with Dye-Enhanced Laser Welding and Fibrinogen (1991)

The topical application of wavelength-specific dye and fibrinogen has been used to enhance laser closure of vascular anastomoses. We compared the closure of skin incisions by two different dye-enhanced, fibrinogen-based laser welding systems [argon laser (power density 4.78 W/cm2) with fluorescein isothiocyanate dye (n = 32) and diode laser (power density 9.55 W/cm2) with indocyanine green dye (n = 32)] with closure by interrupted 5-0 nylon suture (n = 64) and examined tensile strength, hydroxyproline production, histology, and cosmesis. Two 3-cm full-thickness incisions were made on the shaved backs of 64 rats. One incision was closed with suture, whereas the other, after treatment with the appropriate dye, was welded with either argon- or diode-lasered fibrinogen. At postoperative days 5, 10, 15, and 28, the closure sites were harvested and sectioned for analysis. Initially, wounds closed with argon-lasered fibrinogen showed less inflammatory response, greater collagen production (34.61 +/- 0.74 mg/gm), and greater mean peak stress at rupture (64.85 lbs/in2) than those closed with suture (16.42 +/- 3.20 mg/gm, 26.68 lbs/in2) (p less than 0.05). By 15 days, both argon and diode laser closures are superior in strength and collagen production to suture closure (p less than 0.05). At 28 days, diode laser closures (1315.60 lbs/in2) are stronger than suture closures (998.09 lbs/in2), whereas both are stronger than argon laser closures (813.16 lbs/in2) (p less than 0.05). Cosmetically, argon-welded wounds consistently appeared finer and lacked cross-hatched suture scars.(ABSTRACT TRUNCATED AT 250 WORDS)