gebhart/patrepeval huggingface dataset:
The Preparation of Substituted Hydrazines. I. Alkylhydrazines via Alkylsydnones1 (1955)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Preparation of Substituted Hydrazines. I. Alkylhydrazines via Alkylsydnones1Joseph Fugger, Jack M. Tien, and I. Moyer HunsbergerCite this: J. Am. Chem. Soc. 1955, 77, 7, 1843–1848Publication Date (Print):April 1, 1955Publication History Published online1 May 2002Published inissue 1 April 1955https://pubs.acs.org/doi/10.1021/ja01612a039https://doi.org/10.1021/ja01612a039research-articleACS PublicationsRequest reuse permissionsArticle Views621Altmetric-Citations49LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Endothelial Progenitor Cells Are Rapidly Recruited to Myocardium and Mediate Protective Effect of Ischemic Preconditioning via “Imported” Nitric Oxide Synthase Activity (2005)

These findings provide novel insights about the role of bone marrow-derived cells in ischemic preconditioning and also reveal that distinct mechanisms regulate recovery after ischemia-reperfusion and chronic ischemic injury.
Permutation invariant training of deep models for speaker-independent multi-talker speech separation (2017)

We propose a novel deep learning training criterion, named permutation invariant training (PIT), for speaker independent multi-talker speech separation, commonly known as the cocktail-party problem. Different from the multi-class regression technique and the deep clustering (DPCL) technique, our novel approach minimizes the separation error directly. This strategy effectively solves the long-lasting label permutation problem, that has prevented progress on deep learning based techniques for speech separation. We evaluated PIT on the WSJ0 and Danish mixed-speech separation tasks and found that it compares favorably to non-negative matrix factorization (NMF), computational auditory scene analysis (CASA), and DPCL and generalizes well over unseen speakers and languages. Since PIT is simple to implement and can be easily integrated and combined with other advanced techniques, we believe improvements built upon PIT can eventually solve the cocktail-party problem.
Three‐dimensional high‐resolution volume rendering (HRVR) of computed tomography data: Applications to otolaryngology—head and neck surgery (1991)

Conventional computed tomographic display formats are not optimal for demonstrating three-dimensional anatomic relationships. In otolaryngology--head and neck surgery these critical relationships are often highly complex, and their complete understanding is essential to a successful surgical outcome. A new computer-generated image display format, high-resolution volume rendering (HRVR), facilities the understanding of these critical anatomic relationships by transforming conventional imaging data into clinically relevant 3-D images. Unlike many other 3-D reconstruction algorithms, HRVR suffers minimal data loss in the conversion process, which in turn provides for superior image resolution. This better allows the application of 3-D technology to small or complicated anatomic structures such as those frequently encountered in otolaryngology--head and neck surgery. Advances in computer-controlled manipulations that further enhance the evaluation of desired pathologic features have been achieved. This pilot study contains representative clinical cases chosen to illustrate the potential utility of HRVR in otolaryngology--head and neck surgery. The authors believe HRVR images will enhance the surgeon's understanding of the 3-D anatomic relationships that exist between critical pathologic features and surrounding vital structures.
ComEA, a Bacillus subtilis integral membrane protein required for genetic transformation, is needed for both DNA binding and transport (1995)

The competence-related phenotypes of mutations in each of the four open reading frames associated with the comE locus of Bacillus subtilis are described. comEA and comEC are required for transformability, whereas the products of comEB and of the overlapping comER, which is transcribed in the reverse direction, are dispensable. Loss of the comEA product decreases the binding of DNA to the competent cell surface and the internalization of DNA, in addition to exhibiting a profound effect on transformability. The comEC product is required for internalization but is dispensable for DNA binding. ComEA is shown to be an integral membrane protein, as predicted from hydropathy analysis, with its C-terminal domain outside the cytoplasmic membrane. This C-terminal domain possesses a sequence with similarity to those of several proteins known to be involved in nucleic acid transactions including UvrC and a human protein that binds to the replication origin of the Epstein-Barr virus.
Biochemistry of protein-isocyanate interactions: a comparison of the effects of aryl vs. alkyl isocyanates. (1987)

In addition to their use in the polyurethane and pesticide industries, isocyanates have proven to be useful probes for the exploration of protein structure. This paper focuses on three aspects of isocyanates: their broad reactivity, their reversible interaction with cholinesterases, and the relative hydrolysis rates of alkyl and aryl isocyanates. The broad reactivity of isocyanates as well as the demonstrated affinity labeling of serine and sulfhydryl esterases are discussed. Extension of the affinity labeling studies to include the analysis of the inhibition of cholinesterases by methyl isocyanate shows that methyl isocyanate is not an effective inhibitor of any of the cholinesterases. The inhibition of cholinesterases by alkyl isocyanates shows a pattern of decreased specificity with decreased alkyl chain length. The inhibition of cholinesterases by isocyanates is shown to be reversible, with a maximum rate of reversal seen at physiological pH. This reversal is characteristic of the reaction of an isocyanate with a sulfhydryl group. Finally, the affinity labeling of proteins must compete successfully with the hydrolysis of isocyanates in aqueous solution. The hydrolysis of alkyl isocyanates is shown to be significantly slower than that of the aryl isocyanates.
Immortalization and characterization of a nociceptive dorsal root ganglion sensory neuronal line (2007)

Development of neuroprotective strategies for peripheral neuropathies requires high-throughput drug screening assays with appropriate cell types. Currently, immortalized dorsal root ganglion (DRG) sensory neuronal cell lines that maintain nociceptive sensory neuronal properties are not available. We generated immortalized DRG neuronal lines from embryonic day 14.5 rats. Here, we show that one of the immortalized DRG neuronal lines, 50B11, has the properties of a nociceptive neuron. When differentiated in the presence of forskolin, these cells extend long neurites, express neuronal markers, and generate action potentials. They express receptors and markers of small-diameter sensory neurons and upregulate appropriate receptor populations when grown in the presence of glial cell line-derived neurotrophic factor or nerve growth factor. Furthermore, they express capsaicin receptor transient receptor potential vanilloid family-1 (TRPV-1) and respond to capsaicin with increases in intracellular calcium. In a 96-well plate format, these neurons show a decline in ATP levels when exposed to dideoxycytosine (ddC) in a proper time- and dose-dependent manner. This ddC-induced reduction in ATP levels correlates with axonal degeneration. The immortalized DRG neuronal cell line 50B11 can be used for high-throughput drug screening for neuroprotective agents for axonal degeneration and antinociceptive drugs that block TRPV-1.
Characterization of a Double Cellulose-binding Domain (1996)

Most cellulose-degrading enzymes have a two-domain structure that consists of a catalytic and a cellulose-binding domain (CBD) connected by a linker region. The linkage and the interactions of the two domains represent one of the key questions for the understanding of the function of these enzymes. The CBDs of fungal cellulases are small peptides folding into a rigid, disulfide-stabilized structure that has a distinct cellulose binding face. Here we describe properties of a recombinant double CBD, constructed by fusing the CBDs of two Trichoderma reesei cellobiohydrolases via a linker peptide similar to the natural cellulase linkers. After expression in Escherichia coli, the protein was purified from the culture medium by reversed phase chromatography and the individual domains obtained by trypsin digestion. Binding of the double CBD and its single CBD components was investigated on different types of cellulose substrates as well as chitin. Under saturating conditions, nearly 20 micromol/g of the double CBD was bound onto microcrystalline cellulose. The double CBD exhibited much higher affinity on cellulose than either of the single CBDs, indicating an interplay between the two components. A two-step model is proposed to explain the binding behavior of the double CBD. A similar interplay between the domains in the native enzyme is suggested for its binding to cellulase.
Stabilized Sagnac Optical Fiber Current Sensor Using One Phase And Two Amplitude Modulations (2005)

Novel design of a Sagnac optical fiber current sensor using a LiNbO/sub 3/ phase modulator and a special signal processing allowing both measurement and compensation of the linear bireffingence in the fiber.
Nickel-Catalyzed Decarbonylative Addition of Phthalimides to Alkynes (2008)

An intermolecular nickel-catalyzed addition reaction has been developed where N-arylphthalimides react with alkynes to afford substituted isoquinolones. A mechanistic rationale is proposed, implying nucleophilic attack of Ni(0) to an amide as the primary step of the catalytic cycle.
Folding of subtilisin BPN': characterization of a folding intermediate (1993)

Subtilisin BPN', an extracellular serine protease from Bacillus amyloliquefaciens, requires a 77 amino acid pro-sequence for correct folding in vivo. We report the observation of a metastable folding intermediate during the refolding of wild-type and a proteolytically inactive mutant subtilisin BPN' that lack the pro-sequence. The addition of the pro-sequence as a separate polypeptide chain results in the folding of the intermediate to the native state. The intermediate state of subtilisin is stable at different temperatures, pH values, and salt concentrations for more than a week and retains its competence for folding. The intermediate state possesses a compactness between that of the native and unfolded states. Although it has native-like secondary structure, it shows no distinct near-UV CD spectrum and has a strongly reduced dispersion in the amide and methyl regions of the 1H NMR spectrum. These indicate considerably less tertiary structure than possessed by the native state. However, the intermediate conformation has regions of stable tertiary structure: it has a high-affinity calcium binding site and, after a first noncooperative transition, unfolds with guanidinium chloride in a cooperative process. These results support a folding mechanism for subtilisin BPN' that comprises a high energy transition state, which is lowered by the interaction with the pro-sequence. The similarity to the folding mechanism of alpha-lytic protease supports the hypothesis that a common folding mechanism has been developed through convergent evolution.
Use of DNA and Peptide Nucleic Acid Molecular Beacons for Detection and Quantification of rRNA in Solution and in Whole Cells (2003)

DNA and peptide nucleic acid (PNA) molecular beacons were successfully used to detect rRNA in solution. In addition, PNA molecular beacon hybridizations were found to be useful for the quantification of rRNA: hybridization signals increased in a linear fashion with the 16S rRNA concentrations used in this experiment (between 0.39 and 25 nM) in the presence of 50 nM PNA MB. DNA and PNA molecular beacons were successfully used to detect whole cells in fluorescence in situ hybridization (FISH) experiments without a wash step. The FISH results with the PNA molecular beacons were superior to those with the DNA molecular beacons: the hybridization kinetics were much faster, the signal-to-noise ratio was much higher, and the specificity was much better for the PNA molecular beacons. Finally, it was demonstrated that the combination of the use of PNA molecular beacons in FISH and flow cytometry makes it possible to rapidly collect quantitative FISH data. Thus, PNA molecular beacons might provide a solution for limitations of traditional FISH methods, such as variable target site accessibility, poor sensitivity for target cells with low rRNA content, background fluorescence, and applications of FISH in microfluidic devices.
Nanostructured Fe3O4/SWNT Electrode: Binder‐Free and High‐Rate Li‐Ion Anode (2010)

Utilizing Fe3O4 nanorods as the active Li+ storage material and 5 wt% carbon single-walled nanotubes (SWNTs) as a “conductive net,” a Li-ion anode with a high reversible capacity of 1000 mAh g−1 (∼2000 mAh cm−3) at C rate is demonstrated. The electrodes exhibit high-rate capability and stable capacities of 800 mAh g−1 at 5C and ∼600 mAh g−1 at 10C.
Block Copolymer Solid Battery Electrolyte with High Li-Ion Transference Number (2010)

The electrochemical properties of a solid polymer electrolyte consisting of a diblock copolymer and lithium bis(oxalato)borate, (LiBOB) salt, is reported. The spherical microphase-separated diblock copolymer is composed of a majority poly(ethylene oxide) (PEO) block and a minority random copolymer block of methyl methacrylate (MMA) and lithium salt of methacrylic acid (MAALi), . Salt-optimized electrolyte membranes of the diblock copolymer and LiBOB salt exhibited an average lithium-ion transference number value of 0.9 at room temperature . The solid-state flexible, translucent polymer electrolyte shows a wide electrochemical stability window and excellent interface properties with the lithium metal electrode. The combination of these properties makes these block copolymer membranes viable electrolyte candidates for flexible lithium-ion batteries.
Strong Closed-Shell Interactions in Inorganic Chemistry (1997)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStrong Closed-Shell Interactions in Inorganic ChemistryPekka PyykköView Author Information Department of Chemistry, University of Helsinki, P.O.B. 55 (A.I. Virtasen aukio 1), FIN-00014 Helsinki, Finland Cite this: Chem. Rev. 1997, 97, 3, 597–636Publication Date (Web):May 8, 1997Publication History Received3 April 1996Revised14 February 1997Published online8 May 1997Published inissue 1 May 1997https://pubs.acs.org/doi/10.1021/cr940396vhttps://doi.org/10.1021/cr940396vresearch-articleACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views9458Altmetric-Citations2156LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Chemical structure,Gold,Ions,Oligomers,Particulate matter Get e-Alerts
Semiconductor crystal islands for three-dimensional integration (2010)

The critical operation needed to achieve monolithic three-dimensional integrated circuits is obtaining single-crystal, device-quality semiconductor material for upper layer active circuits without exceeding the thermal budget of underlying devices. Previous attempts at using pulsed laser crystallization of amorphous films for upper layer devices failed to provide large enough single crystals at a low enough temperature. Here, the authors demonstrate a more direct approach to realizing high-quality, single-crystal Si (100) and Ge (100) islands (3–3000 μm across) on amorphous SiO2 substrates. The technique is a form of hydrophilic fusion bonding featuring low temperatures (≤400 °C), chemical mechanical polishing, and chemical surface activation (using NH4OH). The bonds are strong enough (&amp;gt;1 J/m2) to withstand SmartCut® removal of the donor wafer. By bonding arrays of islands rather than one large contiguous layer, the authors effectively avoided the formation of thermally induced voids at the bonding interface, and so dramatically improved yield.
Effects of the endogenous clock and sleep time on melatonin, insulin, glucose and lipid metabolism (1998)

This study was undertaken to determine whether the internal clock contributes to the hormone and metabolic responses following food, in an experiment designed to dissociate internal clock effects from other factors. Nine female subjects participated. They lived indoors for 31 days with normal time cues, including the natural light: darkness cycle. For 7 days they retired to bed from 0000 h to 0800 h. They then underwent a 26-h 'constant routine' (CR) starting at 0800 h, being seated awake in dim light with hourly 88 Kcal drinks. They then lived on an imposed 27-h day (18 h of wakefulness, 9 h allowed for sleep), for a total of 27 days. A second 26-h CR, starting at 2200 h, was completed. During each CR salivary melatonin and plasma glucose, triacylglycerol (TAG), non-essential fatty acids (NEFA), insulin, gastric inhibitory peptide (GIP) and glucagon-like peptide-1 (GLP-1) were measured hourly. Melatonin and body temperature data indicated no shift in the endogenous clock during the 27-h imposed schedule. Postprandial NEFA, GIP and GLP-1 showed no consistent effects. Glucose, TAG and insulin increased during the night in the first CR. There was a significant effect of both the endogenous clock and sleep for glucose and TAG, but not for insulin. These findings may be relevant to the known increased risk of cardiovascular disease amongst shift workers.
Collocation extraction based on modifiability statistics (2004)

We introduce a new, linguistically grounded measure of collocativity based on the property of limited modifiability and test it on German PP-verb combinations. We show that our measure not only significantly outperforms the standard lexical association measures typically employed for collocation extraction, but also yields a valuable by-product for the creation of collocation databases, viz. possible structural and lexical attributes. Our approach is language-, structure-, and domain-independent because it only requires some shallow syntactic analysis (e.g., a POS-tagger and a phrase chunker).
Integrated optoelectronic interconnects using liquid-crystal-on-silicon VLSI (1996)

The various optical interconnection schemes that have been developed for the implementation of neural network architectures are reviewed. Volume holography in photorefra.ct.ive crystals is the most powerful interconnection method for this application. The use of liquid crystal smart pixel technology for the realization of the nonlinear activation function of the neurons is described. A novel method for combining liquid crystal devices and photorefractive holograms that leads to a very compact and versatile package, is presented.
The Hepatitis B Virus X Protein Modulates Hepatocyte Proliferation Pathways To Stimulate Viral Replication (2010)

Worldwide, there are over 350 million people who are chronically infected with the human hepatitis B virus (HBV); chronic HBV infections are associated with the development of hepatocellular carcinoma (HCC). The results of various studies suggest that the HBV X protein (HBx) has a role in the development of HBV-associated HCC. HBx can regulate numerous cellular signal transduction pathways, including those that modulate cell proliferation. Many previous studies that analyzed the impact of HBx on cell proliferation pathways were conducted using established or immortalized cell lines, and when HBx was expressed in the absence of HBV replication, and the precise effect of HBx on these pathways has often differed depending on experimental conditions. We have studied the effect of HBx on cell proliferation in cultured primary rat hepatocytes, a biologically relevant system. We demonstrate that HBx, both by itself and in the context of HBV replication, affected the levels and activities of various cell cycle-regulatory proteins to induce normally quiescent hepatocytes to enter the G(1) phase of the cell cycle but not to proceed to S phase. We linked HBx regulation of cell proliferation to cytosolic calcium signaling and HBx stimulation of HBV replication. Cumulatively, our studies suggest that HBx induces normally quiescent hepatocytes to enter the G(1) phase of the cell cycle and that this calcium-dependent HBx activity is required for HBV replication. These studies identify an essential function of HBx during HBV replication and a mechanism that may connect HBV infections to the development of HCC.
Kinetic Properties of “Soluble” Adenylyl Cyclase (2003)

"Soluble" adenylyl cyclase (sAC) is a widely expressed source of cAMP in mammalian cells that is evolutionarily, structurally, and biochemically distinct from the G protein-responsive transmembrane adenylyl cyclases. In contrast to transmembrane adenylyl cyclases, sAC is insensitive to heterotrimeric G protein regulation and forskolin stimulation and is uniquely modulated by bicarbonate ions. Here we present the first report detailing kinetic analysis and biochemical properties of purified recombinant sAC. We confirm that bicarbonate regulation is conserved among mammalian sAC orthologs and demonstrate that bicarbonate stimulation is consistent with an increase in the V(max) of the enzyme with little effect on the apparent K(m) for substrate, ATP-Mg(2+). Bicarbonate can further increase sAC activity by relieving substrate inhibition. We also identify calcium as a direct modulator of sAC activity. In contrast to bicarbonate, calcium stimulates sAC activity by decreasing its apparent K(m) for ATP-Mg(2+). Because of their different mechanisms, calcium and bicarbonate synergistically activate sAC; therefore, small changes of either calcium or bicarbonate will lead to significant changes in cellular cAMP levels.
What's in a Label? Radioimmunotherapy for Metastatic Prostate Cancer (2013)

Abstract Prostate cancer has a highly tumor-restricted prostate-specific membrane antigen (PSMA) and may be the ideal solid-organ malignancy for treatment with radioimmunotherapy. Encouraging results using lutetium-177–labeled anti-PSMA monoclonal antibody J591 from a phase II study by Tawaga and colleagues support the continued clinical and preclinical development of radioimmunotherapy for solid tumors. Clin Cancer Res; 19(18); 4908–10. ©2013 AACR.