gebhart/patrepeval huggingface dataset:
Correlation of surface acoustic wave device coating responses with solubility properties and chemical structure using pattern recognition (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCorrelation of surface acoustic wave device coating responses with solubility properties and chemical structure using pattern recognitionDavid S. Ballantine, Susan L. Rose, Jay W. Grate, and Hank. WohltjenCite this: Anal. Chem. 1986, 58, 14, 3058–3066Publication Date (Print):December 1, 1986Publication History Published online1 May 2002Published inissue 1 December 1986https://pubs.acs.org/doi/10.1021/ac00127a035https://doi.org/10.1021/ac00127a035research-articleACS PublicationsRequest reuse permissionsArticle Views511Altmetric-Citations145LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Simple Method for the Esterification of Carboxylic Acids (1978)

Addition of 4‐dimethylaminopyridine (DMAP) accelerates the dicyclohexylcarbo‐diimide (DCC)‐activated esterification of carboxylic acids to such an extent that side reactions are eliminated and even sensitive acids such 2,5‐cyclohexadiene‐1‐carboxylic acid readily form the tert ‐butyl ester. DMAP has so far been used mainly as acylation catalyst. equation image
T-cell Differentiation and Immunological Homeostasis in Lymphopenic and Kappa Light Chain Deficient Mice (2002)

T lymphocytes are primarily involved in adaptive, cell-mediated, immune reactions. In this thesis T cells were studied regarding central and peripheral differentiation and homeostatic mechanisms for maintanance of the immune repertoire.The influence by mature T cells on thymic development was studied in C.B-17 scid/scid (SCID) mice, devoid of mature T and B cells, and whose thymocyte development is arrested at the early pro-T cell stage. When mature syngeneic T cells were injected the developmental block was overcome and there was an accumulation of CD4+CD8+ thymocytes. This event was accompanied by the maturation of medullary epithelial cells in thymus which seemed to be driven by CD8+ T cells. In the periphery there was initially a spontaneous T-cell proliferation and later, the majority of the donor T lymphocytes showed a memory phenotype with high expression of CD44 and with an early onset of proliferation and cytokine production upon stimulation. This stable pool of memory type of cells sustained for more than a year following treatment.Treating SCID mice with allogeneic T cells results in graft-versus-host disease (GVHD). Severe GVHD was dependent on the MHC-haplotype of the donor cells and was accompanied by profound alterations of the TCR-Vβ repertoire and with high production of IFN-γ.Kappa light chain (κ)-deficient mice have only half the number of B cells as their normal counterparts but normal levels of immunoglobulins. When T cells from κ-deficient mice were stimulated in vitro there was a bias towards production of B-cell stimulatory type 2 cytokines. This is proposed as a mechanism for the homeostatic control of serum immunoglobulin levels in κ-deficient mice.
Anti-TIM3 Antibody Promotes T Cell IFN-γ–Mediated Antitumor Immunity and Suppresses Established Tumors (2011)

Strategies to activate and rescue exhausted tumor-specific T cells, including the use of monoclonal antibodies (mAb) that block the negative costimulatory receptors CTLA-4 and PD-1 are proving very effective, but TIM3 has been relatively neglected as a target. Here we report an extensive characterization of the therapeutic activity and mechanism of action of an anti-mouse TIM3 mAb against experimental and carcinogen-induced tumors. For the first time we specifically define the mechanism of antitumor action of anti-TIM3 requiring IFN-γ producing CD8(+) T cells and CD4(+) T cells, and a higher ratio of tumor infiltrating CD8(+):CD4(+) T cells correlating with therapeutic success. Interestingly, in some models, anti-TIM3 appeared to be effective sometime before the appearance and accumulation of significant TIM3(+)PD-1(+) T cell populations in tumor bearing mice. Anti-TIM3 displayed modest prophylactic and therapeutic activity against a small fraction of carcinogen-induced sarcomas, but comparative and combination studies of anti-TIM3 with anti-CTLA-4 and anti-PD-1 against experimental and carcinogen-induced tumors suggested that these agents might be well-tolerated and very effective in combination.
La planeación didáctica desde el enfoque por competencias en educación básica (2017)

El siguiente documento presenta una mirada a los elementos necesarios para elaborar una planeacion en cualquiera de los niveles de educacion basica bajo el enfoque por competencias, es decir identificar cuales de dichos elementos son realmente trascendentales para potenciar competencias, por lo que se inicia cuestionando ?Cuales son los elementos indispensables dentro de la planificacion para fomentar competencias en los alumnos? Para responder dicha pregunta se utilizo el metodo de investigacion documental de tipo critica valorativa bajo la lectura de comprension con la cual se busco resolver dudas a partir de cuestionamientos criticos implicitos. De esta manera se obtuvieron como resultados la importancia de ident i f i car elementos cur r i culares , contextuales, teoricos y de aplicacion para basar la planeacion en un enfoque por competencias y finalmente materializarlo en un esquema de planeacion que posibilitaria la articulacion de los elementos.
The Neuronal RNA Binding Protein Nova-1 Recognizes Specific RNA Targets In Vitro and In Vivo (1997)

Nova-1, an autoantigen in paraneoplastic opsoclonus myoclonus ataxia (POMA), a disorder associated with breast cancer and motor dysfunction, is a neuron-specific nuclear RNA binding protein. We have identified in vivo Nova-1 RNA ligands by combining affinity-elution-based RNA selection with protein-RNA immunoprecipitation. Starting with a pool of approximately 10(15) random 52-mer RNAs, we identified long stem-loop RNA ligands that bind to Nova-1 with high affinity (Kd of approximately 2 nM). The loop region of these RNAs harbors a approximately 15-bp pyrimidine-rich element [UCAU(N)(0-2)]3 which is essential for Nova-1 binding. Mutagenesis studies defined the third KH domain of Nova-1 and the [UCAU(N)(0-2)]3 element as necessary for in vitro binding. Consensus [UCAU (N)(0-2)], elements were identified in two neuronal pre-mRNAs, one encoding the inhibitory glycine receptor alpha2 (GlyR alpha2) and a second encoding Nova-1 itself. Nova-1 protein binds these RNAs with high affinity and specificity in vitro, and this binding can be blocked by POMA antisera. Moreover, both Nova-1 and GlyR alpha2 pre-mRNAs specifically coimmunoprecipitated with Nova-1 protein from brain extracts. Thus, Nova-1 functions as a sequence-specific nuclear RNA binding protein in vivo; disruption of the specific interaction between Nova-1 and GlyR alpha2 pre-mRNA may underlie the motor dysfunction seen in POMA.
Ultrasound system for measuring position and orientation of laparoscopic surgery tools (2004)

A new ultrasound wireless positioning system is developed that gives the surgeon the exact 3D location and orientation of the instruments in the patient. The measuring system employs ultrasound markers/transmitters placed on the instruments, outside of the human body. Knowing the dimensions of the usually rigid instruments it is possible to calculate their position and orientation inside the patient from the markers' positions. The detection of the marker positions is realized by an array of ultrasound receivers. Using the cross-correlation method for the time of flight determination (transmitter-receiver) we obtained a resolution of 0.7 mm in determining the marker positions. A wireless radio wave system (RF) is used to trigger the ultrasound markers.
Inhibition of anandamide hydrolysis attenuates nociceptor sensitization in a murine model of chemotherapy-induced peripheral neuropathy (2014)

Painful neuropathy frequently develops as a consequence of commonly used chemotherapy agents for cancer treatment and is often a dose-limiting side effect. Currently available analgesic treatments are often ineffective on pain induced by neurotoxicity. Although peripheral administration of cannabinoids, endocannabinoids, and inhibitors of endocannabinoid hydrolysis has been effective in reducing hyperalgesia in models of peripheral neuropathy, including chemotherapy-induced peripheral neuropathy (CIPN), few studies have examined cannabinoid effects on responses of nociceptors in vivo. In this study we determined whether inhibition of fatty acid amide hydrolase (FAAH), which slows the breakdown of the endocannabinoid anandamide (AEA), reduced sensitization of nociceptors produced by chemotherapy. Over the course of a week of daily treatments, mice treated with the platinum-based chemotherapy agent cisplatin developed robust mechanical allodynia that coincided with sensitization of cutaneous C-fiber nociceptors as indicated by the development of spontaneous activity and increased responses to mechanical stimulation. Administration of the FAAH inhibitor URB597 into the receptive field of sensitized C-fiber nociceptors decreased spontaneous activity, increased mechanical response thresholds, and decreased evoked responses to mechanical stimuli. Cotreatment with CB1 (AM281) or CB2 (AM630) receptor antagonists showed that the effect of URB597 was mediated primarily by CB1 receptors. These changes following URB597 were associated with an increase in the endocannabinoid anandamide in the skin. Our results suggest that enhanced signaling in the peripheral endocannabinoid system could be utilized to reduce nociceptor sensitization and pain associated with CIPN.
Kinetic Evidence for the Formation of Acyl-Enzyme Intermediates in the α-Chymotrypsin-Catalyzed Hydrolyses of Specific Substrates (1964)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTKinetic Evidence for the Formation of Acyl-Enzyme Intermediates in the α-Chymotrypsin-Catalyzed Hydrolyses of Specific SubstratesBurt. Zerner, Richard P. M. Bond, and Myron L. BenderCite this: J. Am. Chem. Soc. 1964, 86, 18, 3674–3679Publication Date (Print):September 1, 1964Publication History Published online1 May 2002Published inissue 1 September 1964https://pubs.acs.org/doi/10.1021/ja01072a016https://doi.org/10.1021/ja01072a016research-articleACS PublicationsRequest reuse permissionsArticle Views559Altmetric-Citations140LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Fibronectin and water content of articular cartilage explants after partial depletion of proteoglycans (1986)

The aim of this study was to test the hypothesis that fibronectin in cartilage of osteoarthritic joints accumulates in increased quantities in response to a reduction in proteoglycan content in the matrix. We determined fibronectin content in an ELISA system, uptake of plasma 125I-fibronectin from the medium, and glycosaminoglycan content by a dye method. The proteoglycan content of canine articular cartilage was reduced by co-incubation of the cartilage with canine synovium, partially purified catabolin, or retinoic acid. Data suggested that proteoglycan loss alone was not sufficient to result in the increased fibronectin accumulation that has been reported in the deteriorating articular cartilage of osteoarthritic joints. Cartilage that lost as much as 75% of its proteoglycan content did not have increased fibronectin content and did not take up more fibronectin from the external medium. A related goal of this study was to evaluate the percentage of water in proteoglycan-depleted but disease-free cartilage, as the percentage of water is known to be elevated in osteoarthritic cartilage. Data indicated that the percentage of water in the depleted cartilage increased in proportion to the decrease in proteoglycan content, but the total amount of water in the cartilage did not increase; water content appeared to be unchanged. Under the conditions used, the proteoglycan content was reduced and the percentage of water was elevated, but fibronectin content was not increased. Thus, the cartilage incubated in vitro did not mimic osteoarthritic cartilage.
PROLONGATION OF PRIMATE CARDIAC XENOGRAFT SURVIVAL WITH CYCLOSPORINE (1987)

Cardiac xenotransplantation in nonprimates using traditional immunosuppression (azathioprine and prednisone) or cyclosporine has been unsuccessful or has required doses of immunosuppressants not tolerated by man. This study sought to determine if primate hearts could be transplanted successfully across genus boundaries using a dose of cyclosporine applicable to human transplantation. The hearts of outbred cynomolgus monkeys (Macaca fascicularis) were heterotopically transplanted into the necks of outbred baboons (Papio anubis). Hyperacute rejection did not occur and there were no cyclosporine-induced malignancies or nephrotoxicity. A 12-fold prolongation of mean cardiac xenograft survival to 77 days was accomplished using parenteral cyclosporine and steroids. The histology of rejection was notable for the appearance of reversible rejection on the 30-day biopsies. The histopathologic and immunologic data support the role of both cell-mediated and humoral mechanisms in primate cardiac xenograft rejection. Neither mixed lymphocyte cultures or cytotoxic antibody assays were predictive of graft loss, but there was a significant increase in their respective levels at the time of cessation of graft function. Thus, significant prolongation of primate cardiac graft survival across a genus boundary was accomplished using a dose of cyclosporine similar to that used in human transplantation.
Direct Activation of Cystic Fibrosis Transmembrane Conductance Regulator Channels by 8-Cyclopentyl-1,3-dipropylxanthine (CPX) and 1,3-Diallyl-8-cyclohexylxanthine (DAX) (1998)

8-Cyclopentyl-1,3-dipropylxanthine (CPX) and 1,3-diallyl-8-cyclohexylxanthine (DAX) are xanthine adenosine antagonists which activate chloride efflux from cells expressing either wild-type or mutant (DeltaF508) cystic fibrosis transmembrane conductance regulator (CFTR). These drugs are active in extremely low concentrations, suggesting their possible therapeutic uses in treating cystic fibrosis. However, knowledge of the mechanism of action of these compounds is lacking. We report here that the same low concentrations of both CPX and DAX which activate chloride currents from cells also generate a profound activation of CFTR channels incorporated into planar lipid bilayers. The process of activation involves a pronounced increase in the total conductive time of the incorporated CFTR channels. The mechanism involves an increase in the frequency and duration of channel opening events. Thus, activation by these drugs of chloride efflux in cells very likely involves direct interaction of the drugs with the CFTR protein. We anticipate that this new information will contribute fundamentally to the rational development of these and related compounds for cystic fibrosis therapy.
In vivo induction of IL-6 by administration of exogenous cytokines and detection of de novo serum levels of IL-6 in tumor-bearing mice. (1989)

We investigated the capacity of several recombinant cytokines to induce IL-6 in vivo in both normal and tumor-bearing (TB) mice. Intravenous administration of human rhTNF-alpha, rhIL-1, rhIL-2, rhIFN-alpha A/D, and rmIFN-gamma were all capable of inducing circulating IL-6. rhTNF-alpha administration caused the greatest induction of IL-6. TB animals consistently produced more IL-6 in response to rhTNF-alpha than did normal mice (2 h after 4 micrograms rhTNF-alpha, TB = 24,100 HGF U/ml, non-TB = 3600 HGF U/ml of IL-6). A single daily i.v. dose of rhTNF-alpha (4 micrograms/mouse/day) for 5 days led to decreased IL-6 induction in TB animals by day 3 of treatment (peak levels of IL-6, day 1 = 72,800 HGF U/ml, day 3 = 23,400 HGF U/ml, day 5 = 26,400 HGF U/ml). rhIL-1 administration also resulted in considerable IL-6 production, although peak values were less than those resulting from administration of rhTNF-alpha. Administration of rhIL-1 induced similar IL-6 levels (TB = 10,025 and non-TB = 10,600 HGF U/ml) in TB and normal mice. Single high doses of rhIL-2, rhIFN-alpha A/D, and rmIFN-gamma induced lower but consistent levels of circulating IL-6 in mice with and without tumor. In addition, the sera of untreated TB mice contained levels of IL-6 which paralleled the extent of tumor burden (serum IL-6 in day 30 MCA 106 TB mice = 420 HGF U/ml). The detection of de novo IL-6 was also confirmed in animals bearing tumors of different histologies (the MCA 102 sarcoma, MCA 38 adenocarcinoma, and B16 melanoma). At no time was IL-6 measurable in the sera of untreated normal mice. The identification of IL-6 was verified by neutralization studies using specific antimurine IL-6 antibody. Although the exact role of IL-6 in TB animals remains to be elucidated, its known pleotrophic immune and metabolic effects may be important in the host response to malignancy.
Retinoic‐acid‐induced limb‐reduction defects: Perturbation of zones of programmed cell death as a pathogenetic mechanism (1989)

Pregnant C57Bl/6J mice were treated with 100 mg/kg body weight of all-trans retinoic acid in sesame oil on day 11.0 of gestation. Among the live fetuses harvested on day 18 of gestation, 100% had mesomelic defects of the limbs as determined by gross examination and skeletal staining. Control fetuses treated with sesame oil had no observable limb malformations. Some treated and control embryos were harvested 12 hr after treatment and examined for patterns of cell death by using the supravital stain Nile blue sulphate and methylene-blue- and acid-fuchsin-stained histological sections. Retinoic-acid-induced cell death in the core of the limb was always associated with the zones of programmed cell death as seen in control embryos of comparable stages. This, in concert with previous studies demonstrating excessive cell death in regions of programmed cell death that correlated with subsequent malformations, leads us to conclude that the pathogenesis of mesomelic malformations has a primary association with the phenomenon of programmed cell death.
Using internet video calls in qualitative (longitudinal) interviews: some implications for rapport (2017)

Forming part of the new ‘methodological frontier’ the use of digital communication technologies has become increasingly commonplace in social research. Whilst audio-only online interviews and asynchronous means of communicating online has been discussed for over two decades, video capabilities; being able to see a participant face-to-face online (e.g. via Skype, Face Time, Google Hangouts) is a much more recent phenomenon. Discussion of such new opportunities has tended to centre on the practicalities and technicalities. Alternatively, this paper moves beyond the recent empiricist focus to reflect critically on the implications of using internet video calls on methodological matters of importance to qualitative (longitudinal) researchers. Drawing on a long-standing qualitative longitudinal study following lives of over 50 young people, the paper focuses on the potentials and pitfalls for rapport of using internet video calls for qualitative interviewing. The findings have resonance for short-term studies, and longitudinal endeavours.
Spectres in storage the colonial legacy of art museums (2016)

Donald Preziosi’s investigations of art history and museology work toward unmasking the ideological apparatus of the museum institution. With the current proliferation of museum studies programs in academia, Preziosi’s disciplinary critique becomes a key pedagogical device to illuminate the subject of museum ethics. In classroom discussions on museological practices of collection, repatriation and exhibition, complex subjects that are further complicated by the politics of imperialism and globalization, I propose organizing students to stage mock trials of specific controversies in which the colonial legacy of the museum resurfaces in the accusations leveled at one another by the contending parties. This strategy helps tackle the problematic ethics of museums and their colonial legacies without compromising the nuanced complexity of the subject.
MULTIMODAL INTERFACES THAT PROCESS WHAT COMES NATURALLY (2000)

During multimodal communication, we speak, shift eye gaze, gesture, and move in a powerful flow of communication that bears little resemblance to the discrete key-board and mouse clicks entered sequentially with a graphical user interface (GUI). A pro-found shift is now occurring toward embracing users ’ natural behavior as the center of the human-computer interface. Multimodal interfaces are being developed that permit our highly skilled and coordinated communicative behavior to control system interactions in a more transparent experience than ever before. Our voice, hands, and entire body, once augmented by sensors such as microphones and cameras, are becoming the ultimate transparent and mobile multimodal input devices. The area of multimodal systems has expanded rapidly during the past five years. Since Bolt’s [1] original “Put That There ” concept demonstration, which processed speech and manual pointing during object manipulation, significant achievements have been made Using our highly skilled and coordinated communication patterns to control computers in a more transparent interface experience. in developing more general multimodal systems. State-of-the-art multimodal speech and gesture systems now process complex gestural input other than pointing, and new systems have been extended to process different mode combinations—the most noteworthy being speech and pen input [9], and speech and lip movements [10]. As a foundation for advancing new multimodal systems, proactive empirical work has generated predictive information on human-computer multimodal interaction, which is being used to