gebhart/patrepeval huggingface dataset:
Blockade of the epidermal growth factor receptor signaling inhibits angiogenesis leading to regression of human renal cell carcinoma growing orthotopically in nude mice. (2002)

We determined whether blockade of the epidermal growth factor-receptor (EGF-R) signaling pathway by oral administration of the EGF-R tyrosine kinase inhibitor PKI166 can inhibit angiogenesis and growth of SN12PM6 human renal cell carcinoma (HRCC) in the kidney of nude mice and whether gemcitabine can potentiate these effects. In vitro treatment of HRCC cells with PKI166 inhibited EGF-R autophosphorylation, which correlated with a decrease in expression of Bcl-xl protein and phosphorylation of signal transducers and activators of transcription, particularly signal transducers and activators of transcription 3. PKI166 also decreased expression of vascular endothelial growth factor and basic fibroblast growth factor in a dose-dependent manner. Oral administration of PKI166 or PKI166 and injected gemcitabine or gemcitabine alone beginning 7 days after implantation of SN12PM6 cells into the kidney of athymic nude mice reduced the volume of tumors by 26, 61, and 23%, respectively. In another experiment 28 days after the orthotopic implantation of SN12PM6 cells, nephrectomy was performed followed by 4 weeks of treatment. Treatment with PKI166 and, more so, PKI166 plus gemcitabine significantly inhibited lung metastasis, corresponding to a significant increase in overall length of survival. EGF-R activation was significantly blocked by therapy with PKI166 and was associated with a significant reduction in expression of vascular endothelial growth factor and interleukin-8, decreased microvessel density, decreased staining of proliferating cell nuclear antigen, and increased tumor cell apoptosis. Collectively, the data indicate that targeting activation of EGF-R on HRCC produces significant therapeutic benefits.
Local coordination structure and electronic structure of the large electron mobility amorphous oxide semiconductor In-Ga-Zn-O: Experiment andab initiocalculations (2007)

Ionic amorphous oxide semiconductors (IAOSs) are new materials for flexible thin film transistors that exhibit field-effect mobilities of $\ensuremath{\sim}10\phantom{\rule{0.3em}{0ex}}{\mathrm{cm}}^{2}\phantom{\rule{0.2em}{0ex}}{\mathrm{V}}^{\ensuremath{-}1}\phantom{\rule{0.2em}{0ex}}{\mathrm{s}}^{\ensuremath{-}1}$ [K. Nomura et al., Nature 488, 432 (2004)]. The local coordination structure in an IAOS, In-Ga-Zn-O ($a$-IGZO), was examined using extended x-ray absorption fine structure analysis combined with ab initio calculations. The short-range ordering and coordination structures in $a$-IGZO are similar to those in the corresponding crystalline phase, $\mathrm{In}\mathrm{Ga}\mathrm{Zn}{\mathrm{O}}_{4}$, and edge-sharing structures consisting of In-O polyhedra remain in the amorphous structure. The ${\mathrm{In}}^{3+}$ $5s$ orbitals form an extended state with a band effective mass of $\ensuremath{\sim}0.2{m}_{e}$ at the conduction band bottom.
Initial fine mapping of the spinach downy mildew resistance locus RPF1 (2013)

Spinach is an important vegetable crop grown in many parts of the world (Correll et al. 2011). It has become increasingly popular due to its nutritional value as it is very high in antioxidants (Prior, 2003). Spinach production, however, is affected by many biotic stresses. Downy mildew, caused by Peronospora farinosa f. sp. spinaciae (Pfs) , is perhaps the most important biotic constraint for spinach production worldwide (Correll et al, 1994). As of 2012, 14 races of Pfs have been reported (Correll et al., 2011). The rapid increase of new races is likely a direct result of substantial changes in spinach production during the past decade, including a rapid expansion of planted acreage, 12-month per year production cycles, high density plantings, reduced usage of rotation crops, and possibly the global movement of the pathogen either as mycelium in seed or as dormant oospores (Inaba and Morinaka, 1984; Inaba et al., 1983). Very few studies have been published on the genetics of resistance to Pfs . However, several more recent studies on the genetics of resistance have been conducted and it has been hypothesized that there are at least six resistance loci (RPF1-RPF6) which control resistance to Pfs races 1-14 (Correll et al., 2007; 2011; Irish et al., 2008). Irish et al. (2008) also identified a molecular marker, designated Dm-1, which was closely linked to the resistance locus RPF1. The Dm-1 marker was a co-dominant marker and the genetic distance between Dm-1 and RPF1 was estimated to be 1.7 cM. In the current study, efforts were initiated to fine-map the Dm-1 marker relative to the RPF1 locus. To address this objective, a spinach BAC library that was previously developed from line NIL1 containing Dm-1 and RPF1 was evaluated. Southern blot hybridizations identified 19 BAC clones that contained Dm-1. Dm-1 was subsequently confirmed in these 19 clones based on PCR analysis. Out of over 75,000 clones evaluated, BAC-end sequencing was conducted on 14 of the 19 BAC clones and the sequence information was used to develop probes for Southern hybridizations. Hybridizations with the 28 probes developed were conducted to identify the BAC-end furthest from the Dm-1 marker. These activities provided important sequence information about the region surrounding the Dm-1 marker, and refined a protocol to map and ultimately clone the RPF1 locus through BAC-based chromosome walking combined with genetic mapping.
Towards Curtailing Speaker Anxiety Via Impromptu and Oral Interpretation (2015)

This article outlines a series of short oral assignments designed to help alleviate some of the anxiety that many students face when giving public presentations. The assignments consist of: giving an impromptu speech, giving a spoken performance of a song or poem of their choice, and a short reading from a children’s book. Each of these assignments are seen as building blocks, methods for reducing nervousness, methods for building confidence, while concurrently developing peer support as students are working towards a more formal presentation later in the semester. There should be a class debriefing/discussion after all three assignments have been completed. Here, students share their personal experiences and provide ideas to each other for successfully presenting their final speeches.
Cholecystokinin octapeptide: a potential growth factor for pancreatic beta cells in diabetic rats. (2004)

CCK-8 could improve blood glucose concentrations in type 1 diabetic rats correlated with an increase in beta cell mass probably potentiated by the chronic hyperglycemic state.
Hydrophobic polyoxins are resistant to intracellular degradation in Candida albicans (1986)

Two novel polyoxins, N-epsilon-(octanoyl)-lysyl-uracil polyoxin C (Oct-Lys-UPOC) and N-gamma-(octyl)-glutaminyluracil polyoxin C (Oct-Gln-UPOC), were synthesized by reacting uracil polyoxin C with the appropriate amino acid p-nitrophenyl ester. Oct-Lys-UPOC and Oct-Gln-UPOC were strong inhibitors (Kis = 1.7 X 10(-6)M) of chitin synthetase from Candida albicans membrane preparations. In a permeabilized-cell assay, Oct-Gln-UPOC had a 10-fold-lower inhibitory activity toward chitin synthetase than did the Oct-Lys-UPOC analog. Both compounds were resistant to hydrolysis by a cell extract of C. albicans H317; however, Oct-Gln-UPOC was hydrolyzed with a half-life of 23 min by a permeabilized-cell preparation. Oct-Lys-UPOC was resistant to hydrolysis by permeabilized cells. Oct-Gln-UPOC and Oct-Lys-UPOC did not compete with the transport of peptides or uridine into the cell. At concentrations up to 2 mM these two new polyoxins were ineffective in the inhibition of cell growth or reduction of cell viability, but they induced aberrant morphologies in C. albicans at a concentration of 0.25 mM. These data suggest that polyoxins containing hydrophobic amino acids retain strong chitin synthetase inhibitory activity and are resistant to cellular hydrolysis. They provide the first example of effective synthetic chitin synthetase inhibitors which are stable inside C. albicans.
Recombinant R-spondin2 and Wnt3a Up- and Down-Regulate Novel Target Genes in C57MG Mouse Mammary Epithelial Cells (2012)

R-spondins (Rspos) comprise a family of four secreted proteins that have important roles in cell proliferation, cell fate determination and organogenesis. Rspos typically exert their effects by potentiating the Wnt/β-catenin signaling pathway. To systematically investigate the impact of Rspo/Wnt on gene expression, we performed a microarray analysis using C57MG mouse mammary epithelial cells treated with recombinant Rspo2 and/or Wnt3a. We observed the up- and down-regulation of several previously unidentified target genes, including ones that encode proteins involved in immune responses, effectors of other growth factor signaling pathways and transcription factors. Dozens of these changes were validated by quantitative real time RT-PCR. Time course experiments showed that Rspo2 typically had little or no effect on Wnt-dependent gene expression at 3 or 6 h, but enhanced expression at 24 h, consistent with biochemical data indicating that Rspo2 acts primarily to sustain rather than acutely increase Wnt pathway activation. Up-regulation of gene expression was inhibited by pre-treatment with Dickkopf1, a Wnt/β-catenin pathway antagonist, and by siRNA knockdown of β-catenin expression. While Dickkopf1 blocked Rspo2/Wnt3a-dependent down-regulation, a number of down-regulated genes were not affected by β-catenin knockdown, suggesting that in these instances down-regulation was mediated by a β-catenin-independent mechanism.
Novel Mutual Prodrug of Retinoic and Butyric Acids with Enhanced Anticancer Activity (2000)

Acyloxylalkyl esters of retinoic acid and small carboxylic acids (C3-5) were evaluated for anticancer activity. The derivative of butyric acid (BA) and all-trans-retinoic acid (ATRA)-retinoyloxymethyl butyrate (RN1)-acting as a mutual prodrug was a more potent inducer of cancer cell differentiation and inhibitor of proliferation than the parent acids. ED50 of RN1 for differentiation induction in HL-60 was over 40-fold lower than that of ATRA. The differentiating activity of ATRA compared to that of the acyloxylalkyl esters derived from butyric (RN1), propionic (RN2), isobutyric (RN3), and pivalic (RN4) acids was found to be: RN1 > RN2 > RN3 > ATRA approximately RN4. This observation implies that the activity of the prodrugs depends on the specific acyl fragment attached to the retinoyl moiety, and the butyroyl fragment conferred the highest potency. The IC50 values for inhibition of Lewis lung (3LLD122) and pancreatic (PaCa2) carcinoma cell line colony formation elicited by RN1 were significantly higher than those of ATRA. In addition to its superiority over ATRA or BA as growth inhibitors of the above cell lines, RN1 was also able to overcome the resistance to ATRA in 3LLD122 cells.
Role of Purine Nucleoside Phosphorylase in Interactions between 2′,3′-Dideoxyinosine and Allopurinol, Ganciclovir, or Tenofovir (2004)

ABSTRACT The level of systemic exposure to 2′,3′-dideoxyinosine (ddI) is increased 40 to 300% when it is coadministered with allopurinol (Allo), ganciclovir (GCV), or tenofovir. However, the mechanism for these drug interactions remains undefined. A metabolic route for ddI clearance is its breakdown by purine nucleoside phosphorylase (PNP). Consistent with previous reports, enzymatic inhibition assays showed that acyclic nucleotide analogs can inhibit the phosphorolysis of inosine. It was further established that the mono- and diphosphate forms of tenofovir were inhibitors of PNP-dependent degradation of ddI ( K i s, 38 nM and 1.3 μM, respectively). Allo and its metabolites were found to be relatively weak inhibitors of PNP ( K i s, &gt;100 μM). Coadministration of tenofovir, GCV, or Allo decreased the amounts of intracellular ddI breakdown products in CEM cells, while they increased the ddI concentrations (twofold increase with each drug at approximately 20 μM). While inhibition of the physiological function of PNP is unlikely due to the ubiquitous presence of high levels of enzymatic activity, phosphorylated metabolites of GCV and tenofovir may cause the increased level of exposure to ddI by direct inhibition of its phosphorolysis by PNP. The discrepancy between the cellular activity of Allo and the weak enzyme inhibition by Allo and its metabolites may be explained by an indirect mechanism of PNP inhibition. This mechanism may be facilitated by the unfavorable equilibrium of PNP and the buildup of one of its products (hypoxanthine) through the inhibition of xanthine oxidase by Allo. These findings support the inhibition of PNP-dependent ddI degradation as the molecular mechanism of these drug interactions.
A high-resolution head-related transfer function and three-dimensional ear model database (2016)

Nowadays immersive three-dimensional virtual scenes have become very popular. This paper presents and provides a database of 48 head-related transfer function (HRTF) datasets and the corresponding three-dimensional ear mesh models of individual subjects, in order to select or model an HRTF dataset for binaural synthesis and auditory reproduction. For easy access, the database of the Institute of Technical Acoustics (RWTH Aachen University, Germany) is accessible online. The HRTF measurement setup and the generation of the three-dimensional models of the ear geometry, reconstructed from magnetic resonance imaging scans, are specifically described in depth.
Immunoassays with rolling circle DNA amplification: A versatile platform for ultrasensitive antigen detection (2000)

We describe an adaptation of the rolling circle amplification (RCA) reporter system for the detection of protein Ags, termed "immunoRCA. " In immunoRCA, an oligonucleotide primer is covalently attached to an Ab; thus, in the presence of circular DNA, DNA polymerase, and nucleotides, amplification results in a long DNA molecule containing hundreds of copies of the circular DNA sequence that remain attached to the Ab and that can be detected in a variety of ways. Using immunoRCA, analytes were detected at sensitivities exceeding those of conventional enzyme immunoassays in ELISA and microparticle formats. The signal amplification afforded by immunoRCA also enabled immunoassays to be carried out in microspot and microarray formats with exquisite sensitivity. When Ags are present at concentrations down to fM levels, specifically bound Abs can be scored by counting discrete fluorescent signals arising from individual Ag-Ab complexes. Multiplex immunoRCA also was demonstrated by accurately quantifying Ags mixed in different ratios in a two-color, single-molecule-counting assay on a glass slide. ImmunoRCA thus combines high sensitivity and a very wide dynamic range with an unprecedented capability for single molecule detection. This Ag-detection method is of general applicability and is extendable to multiplexed immunoassays that employ a battery of different Abs, each labeled with a unique oligonucleotide primer, that can be discriminated by a color-coded visualization system. ImmunoRCA-profiling based on the simultaneous quantitation of multiple Ags should expand the power of immunoassays by exploiting the increased information content of ratio-based expression analysis.
Tunnel transistor (1973)

A tunnel transistor which can be easily obtained by the conventional silicon planar technology will be proposed. It promises fine control of electrical characteristics and high yield. The yield per one wafer in the wafer checking has amounted to 100 percent.
Dopamine D2–Receptor Imaging With 123I-Iodobenzamide SPECT in Migraine Patients Abusing Ergotamine: Does Ergotamine Cross The Blood Brain Barrier? (1993)

Two migraine patients were studied by in vivo SPECT using the dopamine D2-receptor specific radioligand 123I-3-iodo-6-methoxybenzamide (123I-IBZM) during ergotamine abuse and after withdrawal. Results were compared with 15 healthy controls. Striatum/cerebellum and striatum/occipital cortex ratios of count rate density were calculated as a semiquantitative measurement for striatal dopamine D2-receptor binding potential. No differences were found in striatal uptake of 123I-IBZM between healthy controls and the patients when on or off ergotamine. Preliminary evidence suggests that ergotamine may not occupy striatal dopamine D2-receptors to a large extent and thus may not cross the blood brain barrier in large quantities.
Nitric Oxide Regulation of Tissue Free Radical Injury (1996)

We have presented evidence from a broad range of chemical, cell biological, and in vivo studies showing that .NO can mediate tissue-protective reactions during oxidant stress, as well as toxic and tissue prooxidant effects. One predominant factor that has been identified which influences .NO being protective versus toxic is the relative rates of production and concentrations of .NO and the more "traditional" family of reactive oxygen species, including O2.-, H2O2, .OH, LO., LOO., and high valency complexes of iron. Also, since so many anti-neutrophil actions of .NO have been described, it is likely that .NO will serve a protective role in acute inflammatory reactions. One issue is certain--many new truths remain to be revealed, as we continue to develop our understanding of the toxicology of reactive oxygen- and nitrogen-containing species.
Acceleration of Diabetic Wound Healing by an Angiopoietin Peptide Mimetic (2008)

Angiopathies are one of the leading underlying causes of morbidity in diabetic patients. Poorly managed blood glucose levels contribute to vascular defects that manifest themselves in numerous different clinical conditions, including diabetic retinopathy, nephropathy, peripheral artery disease, and compromised wound healing. The angiopoietin family (Angs 1-4) has been shown to play a critical role in the growth and maintenance of vasculature. Here we evaluate the efficacy of a new Ang-based peptidomimetic compound, Vasculotide, on diabetic-related wound healing. Stimulation of endothelial cells (ECs) with Vasculotide results in activation of the Ang receptor, Tie 2, and its associated signaling pathways. This activation promoted biological responses such as EC survival, migration, and matrix metalloproteinase 2 (MMP2) production. We show that Vasculotide alone and in combination with vascular endothelial growth factor (VEGF) results in the production of well-arborized vessels supported by myogenic cells. Using an excisional skin-wound model produced on the back of diabetic B6.Cg-m(+/+)Lepr(db)/J (db/db) mice, we found that Vasculotide-treated wounds presented with decreased wound closure times (p < 0.05) and dramatic increases in granulation tissue (p < 0.01). Although the potential of this novel proangiogenic compound in treating microvascular dysfunction is not strictly limited to topical administration, we provide mechanistic evidence as a proof of principle in support of its efficacious use in diabetic wound healing.
Lithium-ion batteries. A look into the future (2011)

A critical overview of the latest developments in the lithium ion batteries technology is reported. We first describe the evolution in the electrolyte area with particular attention to ionic liquids, discussing the expected application of these room temperature molten salts and listing the issues that still prevent their practical implementation. The attention is then focused on the electrode materials presently considered the most promising for enhancing the energy density of the batteries. At the anode side a discussion is provided on the status of development of high capacity tin and silicon lithium alloys. We show that the morphology that is the most likely to ensure commercial exploitation of these alloy electrodes is that involving carbon-based nanocomposites. We finally touch on super-high-capacity batteries, discussing the key cases of lithium-sulfur and lithium-air and attempting to forecast their chances to eventually reach the status of practically appealing energy storage systems. We conclude with a brief reflection on the amount of lithium reserves in view of its large use in the case of global conversion from gasoline-powered cars to hybrid and electric cars.
Multiframe blind deconvolution of astronomical images (1993)

Maximum-likelihood estimation techniques are presented for the problem of forming object estimates from turbulence-degraded images when the point-spread functions are unknown. The inability of unconstrained maximum-likelihood methods to form meaningful estimates is acknowledged, and iterative algorithms are derived for estimating the object by using both a penalized maximum-likelihood method and a physically meaningful parameterization of the point-spread functions by phase errors distributed over an aperture.
Antibodies to the common leukocyte antigen (T200) inhibit an early phase in the activation of resting human B cells. (1987)

T191, a monoclonal antibody reactive with the T200 common leukocyte antigen, profoundly inhibits an early event(s) associated with alpha-immunoglobulin M (alpha IgM)/T cell replacing factor (TRF) or alpha IgM/recombinant interleukin 1 and 2 (rIL 1 and rIL 2)-induced tonsillar B cell proliferation. Kinetic analysis of T191-mediated inhibition indicated that the antibody exerts its effect within 12 to 24 hr of the initiation of cultures and rapidly loses its activity thereafter. Small resting B cells are most sensitive to T191 inhibition, whereas B cells with increasing buoyant density (presumably reflecting stages of increased activation) become progressively T191 insensitive. Analysis of RNA synthesis subsequent to alpha IgM crosslinking of surface immunoglobulin demonstrated that T191 reduced [3H]uridine incorporation by up to 38% during the first 20 hr of culture. In contrast to the effects seen with alpha IgM stimulated B cells, T191 had no inhibitory effect upon phorbol myristate acetate-induced B cell proliferation. The inhibitory effect upon B cell proliferation observed with T191 is not unique among other alpha-T200 antibodies. Four of five previously described alpha-T200 monoclonal antibodies had similar inhibitory effects (82 to 57% maximum inhibition of [3H]thymidine incorporation). However, 13.3, an alpha-T200 monoclonal antibody previously shown to block natural killer (NK) cell-mediated killing was without effect. Likewise, those antibodies capable of inhibiting B cell proliferation failed to block NK-mediated cytolysis. Antibody binding experiments together with proliferation inhibition studies suggest that all of the monoclonal antibodies tested recognized distinct epitopes on the T200 antigen. Both observations are of significance because they demonstrate that the effects seen with anti-T200 antibodies represent an interference with highly specific functional regions on the T200 molecules.