gebhart/patrepeval huggingface dataset:
Sulphydryl blocker induced small intestinal inflammation in rats: a new model mimicking Crohn’s disease (1997)

Jejunal inflammation induced by the sulphydryl blocker, iodoacetamide, resembles the pathological changes in Crohn's disease. The protective effect of ketotifen and apocynin indicates the contribution of O2- and pro-inflammatory mediators to the pathogenesis of the damage, and may be a novel approach to the treatment of inflammatory bowel disease.
Prospective acquisition correction for head motion with image‐based tracking for real‐time fMRI (2000)

In functional magnetic resonance imaging (fMRI) head motion can corrupt the signal changes induced by brain activation.This paper describes a novel technique called Prospective Acquisition Cor-rEction (PACE) for reducing motion-induced effects on magnetization history.Full three-dimensional rigid body estimation of head movement is obtained by image-based motion detection to a high level of accuracy.Adjustment of slice position and orientation, as well as regridding of residual volume to volume motion, is performed in real-time during data acquisition.Phantom experiments demonstrate a high level of consistency (translation < 40m; rotation < 0.05°) for detected motion parameters.In vivo experiments were carried out and they showed a significant decrease of variance between successively acquired datasets compared to retrospective correction algorithms.
ACP-103, a 5-HT2A receptor inverse agonist. (2006)

ACADIA Pharmaceuticals is developing ACP-103, lead compound in a series of 5-HT2A inverse agonists, as a potential antipsychotic agent and for the potential treatment of insomnia. Phase II clinical trials in treatment-induced psychosis in Parkinson's disease (PD) patients and in schizophrenic patients are ongoing, as are phase II trials evaluating the effects of the drug on PD symptoms and dyskinesias.
Lipoic Acid and N-acetyl Cysteine Decrease Mitochondrial-Related Oxidative Stress in Alzheimer Disease Patient Fibroblasts (2007)

In this study, we evaluated the effect of lipoic acid (LA) and N-acetyl cysteine (NAC) on oxidative [4-hydroxy-2-nonenal, N(epsilon)-(carboxymethyl)lysine and heme oxygenase-1] and apoptotic (caspase 9 and Bax) markers in fibroblasts from patients with Alzheimer disease (AD) and age-matched and young controls. AD fibroblasts showed the highest levels of oxidative stress, and the antioxidants, lipoic acid (1 mM) and/or N-acetyl cysteine (100 microM) exerted a protective effect as evidenced by decreases in oxidative stress and apoptotic markers. Furthermore, we observed that the protective effect of LA and NAC was more pronounced when both agents were present simultaneously. AD-type changes could be generated in control fibroblasts using N-methylprotoporphyrin to inhibit cytochrome oxidase assembly indicating that the the oxidative damage observed was associated with mitochondrial dysfunction. The effects of N-methylprotoporphyrine were reversed or attenuated by both lipoic acid and N-acetyl cysteine. These data suggest mitochondria are important in oxidative damage that occurs in AD. As such, antioxidant therapies based on lipoic acid and N-acetyl cysteine supplementation may be promising.
Topical acetylsalicylic acid versus lidocaine for postherpetic neuralgia: results of a double-blind comparative clinical trial. (1999)

A double-blind comparative clinical trial was performed with topical aspirin versus lidocaine for the treatment of 40 patients with postherpetic neuralgia. The percentage improvement following topical aspirin application was 72.2 +/- 19.9 S.D., while with lidocaine it was 72.8 +/- 25.3 S.D. These results suggest that the effect of topical treatment with aspirin is as good as that with lidocaine, since there was no significant difference (P = 0.778) between the two drugs in respect of pain reduction and accordingly the topical application of acetylsalicylic acid can be equally recommended.
The ubiquitin E3 ligase Nedd4-1 controls neurite development (2010)

Comment on: Kawabe H, Neeb A, Dimova K, Young SM Jr, Takeda M, Katsurabayashi S, et al. Regulation of Rap2A by the ubiquitin ligase Nedd4-1 controls neurite development. Neuron 2010; 65:358-72.
Electron field emission from amorphous tetrahedrally bonded carbon films (1996)

Electron field emission from two amorphous, tetrahedrally bonded diamondlike carbon films, one with (a-tC:N), and a second without nitrogen doping (a-tC), prepared by pulsed laser deposition has been investigated using a scanning probe apparatus with micrometer spatial resolution. Electric fields of 100 V/μm (180 V/μm) were required to initiate emission from our a-tC:N (a-tC) films; however, once emission was established at a particular location, electrons could be drawn at average fields as low as 10 V/μm (60 V/μm) from the same region. The initiation of emission was concomitant with electrical discharges which were observed by video techniques. These discharges left craters with micrometer dimensions on the surfaces of otherwise smooth films.
A diode pumped solid state laser driver for inertial fusion energy (1996)

A comprehensive conceptual design for a diode pumped solid state laser (DPSSL) as a driver for an inertial fusion energy (IFE) power plant is presented. This design is based on recent technical advances that offer potential solutions to difficulties previously associated with the use of a laser for IFE applications. The design was selected by using a systems analysis code that optimizes a DPSSL configuration by minimizing the calculated cost of electricity (COE). The code contains the significant physics relevant to the DPSSL driver, but treats the target chamber and balance of plant costs generically using scaling relations published for the Sombrero KrF laser concept. The authors describe the physics incorporated in the code, predict DPSSL performance and its variations with changes in the major parameters, discuss IFE economics and technical risk, and identify the high leverage development efforts that can make DPSSL driven IFE plants more economically competitive. It is believed that this study is a significant advance over previous conceptual studies of DPSSLs for IFE because it incorporates a new cost effective gain medium, applies a potential solution to the `final optics` problem, and considers the laser physics in substantially greater detail. The result is the introduction of an option for an IFE driver that has relatively low development costs and that builds upon the mature laser technology base already developed for Nova and being developed for the proposed National Ignition Facility. The baseline design of the paper has a product of laser efficiency and target gain of ηG~6.6 and a COE of 8.6 cents/kW.h for a 1 GW(e) plant with a target gain of 76 at 3.7 MJ. Higher ηG(≳11) and lower COEs (≲6.6 cents/kW.h) can be achieved with target gains twice as high
Reaginic antibody formation in the mouse. VII. Depression of the ongoing IgE antibody formation by suppressor T cells. (1976)

The ongoing IgE antibody formation against ovalbumin (OA) in high responder mice was depressed by i.v. injections of either native or urea-denatured ovalbumin (UD-OA). Adoptive transfer experiments to determine the helper function of spleen cells from the treated animals showed that helper function for both IgE and IgG antibody responses diminished after treatment. Evidence was obtained that treatment suppressed the expansion of IgE-G memory cells. When the same treatment with OA or UD-OA was given to OA-primed mice before the appearance of IgE antibody in their serum, OA-specific splenic suppressor T cells were demonstrable. Thus, the transfer of splenic T cells from treated mice into normal mice suppressed the primary IgE and IgG antibody responses of the recipeints to DNP-OA. It was also found that the transfer of the splenic T cells from UD-OA-treated mice into OA-primed mice depressed ongoing IgE antibody formation in the recipients. The results suggested strongly that the decrease of helper function and the depression of ongoing IgE antibody formation by repeated injections of UD-OA was caused by generation of antigen (OA)-specific suppressor T cells.
The Novel Nitric Oxide Synthase Inhibitor 4-amino-tetrahydro-L-biopterine Prevents Brain Edema Formation and Intracranial Hypertension following Traumatic Brain Injury in Mice (2009)

Brain edema formation, resulting in increased intracranial pressure (ICP), is one of the most deleterious consequences of traumatic brain injury (TBI). Nitric oxide (NO) has previously been shown to be involved in the damage of the blood-brain barrier (BBB) and, thus, in the formation of post-traumatic brain edema; however, this knowledge never resulted in a clinically relevant therapeutic option because available NO synthase inhibitors have serious side effects in man. The aim of the current study was to investigate the therapeutic efficacy of VAS203, a novel tetrahydrobiopterine (BH3)-based NOS inhibitor, in experimental TBI. When added to isolated vessels rings obtained from rat basilar and middle cerebral arteries (n = 32-35) VAS203 showed the same vasoconstrictive effect as the classical NO synthase inhibitor L-(G)-nitro-arginine-methylester (L-NAME). VAS203 passed the BBB both in healthy and traumatized mouse brain (C57/BL6, n = 5 per group) and did not show any systemic side effects at therapeutic concentrations. When administered 30 min after experimental TBI (controlled cortical impact, 2.2 mg/kg/min i.v., n = 7 per group), VAS203 prevented any further increase in ICP or deterioration of cerebral blood flow. This effect was dose-dependent and long-lasting (i.e., 24 h after trauma, brain edema formation was still significantly reduced [-40%, p < 0.008; n = 7 per group] and functional improvements were present up to 7 days after TBI [p < 0.02 on post-trauma day 6; n = 8 per group]). Therefore, VAS203 may represent a promising candidate for the treatment of acute intracranial hypertension following TBI.
Comparison of Picornaviral IRES-Driven Internal Initiation of Translation in Cultured Cells of Different Origins (1997)

We recently compared the efficiency of six picornaviral internal ribosome entry segments (IRESes) and the hepatitis C virus (HCV) IRES for their ability to drive internal initiation of translationin vitro. Here we present the results of a similar comparison performed in six different cultured cell lines infected with a recombinant vaccinia virus expressing the T7 polymerase and transfected with dicistronic plasmids. The IRESes could be divided into three groups: (i) the cardiovirus and aphthovirus IRESes (and the HCV element) direct internal initiation efficiently in all cell lines tested; (ii) the enterovirus and rhinovirus IRESes are at least equally efficient in several cell lines, but are extremely inefficient in certain cell types; and (iii) the hepatitis A virus IRES is incapable of directing efficient internal initiation in any of the cell lines used (including human hepatocytes). These are the same three groups found when IRESes were classified according to their activitiesin vitro, or according to sequence homologies. In a mouse neuronal cell line, the poliovirus and other type I IRESes were not functional in an artificial bicistronic context. However, infectious poliovirions were produced efficiently after transfection of these cells with a genomic length RNA. Furthermore, activity of the type I IRESes was dramatically increased upon co-expression of the poliovirus 2A proteinase, demonstrating that while IRES efficiency may vary considerably from one cell type to another, at least in some cases viral proteins are capable of overcoming cell-specific translational defects.
An efficient parallel motion estimation algorithm for digital image processing (1991)

The authors present an efficient block-matching algorithm called the parallel hierarchical one-dimensional search (PHODS) for motion estimation. Instead of finding the two-dimensional motion vector directly, the PHODS finds two one-dimensional displacements in parallel on the two axes (say x and y) independently within the search area. The major feature of this algorithm is that its search speed for the motion vector is faster than that of the other search algorithms on account of its simpler computations and parallelism. Compared with previous research in terms of four measurements, the PHODS can rival those algorithms for performance. The hardware-oriented features of the PHODS, i.e., regularity, simplicity, and parallelism, guarantee that the PHODS is more suitable for hardware realization of a VLSI motion estimator.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Calcium Sparks: Elementary Events Underlying Excitation-Contraction Coupling in Heart Muscle (1993)

Spontaneous local increases in the concentration of intracellular calcium, called "calcium sparks," were detected in quiescent rat heart cells with a laser scanning confocal microscope and the fluorescent calcium indicator fluo-3. Estimates of calcium flux associated with the sparks suggest that calcium sparks result from spontaneous openings of single sarcoplasmic reticulum (SR) calcium-release channels, a finding supported by ryanodine-dependent changes of spark kinetics. At resting intracellular calcium concentrations, these SR calcium-release channels had a low rate of opening (approximately 0.0001 per second). An increase in the calcium content of the SR, however, was associated with a fourfold increase in opening rate and resulted in some sparks triggering propagating waves of increased intracellular calcium concentration. The calcium spark is the consequence of elementary events underlying excitation-contraction coupling and provides an explanation for both spontaneous and triggered changes in the intracellular calcium concentration in the mammalian heart.
Management of the potato cyst nematode (Globodera pallida) with bio-fumigants/stimulants. (2007)

Field trials evaluated the effect of four plant-based bio-fumigants/stimulants on population levels of G. pallida and the resulting potato yields and quality. Three formulations contained seaweed biostimulants (Algifol, Nutridip and Metastim) and one bio-fumigant containing mustard and chilli pepper extracts (Dazitol). These were compared with the fumigant nematicide Nemathorin and untreated control plots. The effect of G. pallida on growing potato crops was assessed by recording haulm characteristics which indicated that the nematicide treatment gave most protection. Levels of PCN juveniles and migratory nematodes were assessed during the trial. Plots treated with Nemathorin and Dazitol had fewest PCN, whilst the highest number of migratory nematodes occurred in fallow plots. Sixteen weeks after planting the nematicide treatment produced highest yield and tuber numbers. Dazitol treatment produced a lower yield but the largest tubers.
Gene Transfer of an Engineered Transcription Factor Promoting Expression of VEGF-A Protects Against Experimental Diabetic Neuropathy (2006)

Peripheral neuropathy is a common, irreversible complication of diabetes. We investigated whether gene transfer of an engineered zinc finger protein transcription factor (ZFP-TF) designed to upregulate expression of the endogenous vascular endothelial growth factor (VEGF)-A gene could protect against experimental diabetic neuropathy. ZFP-TF-driven activation of the endogenous gene results in expression of all of the VEGF-A isoforms, a fact that may be of significance for recapitulation of the proper biological responses stimulated by this potent neuroprotective growth factor. We show here that this engineered ZFP-TF activates VEGF-A in appropriate cells in culture and that the secreted VEGF-A protein induced by the ZFP protects neuroblastoma cell lines from a serum starvation insult in vitro. Importantly, single and repeat intramuscular injections of formulated plasmid DNA encoding the VEGF-A-activating ZFP-TF resulted in protection of both sensory and motor nerve conduction velocities in a streptozotocin-induced rat model of diabetes. These data suggest that VEGF-A-activating ZFP-TFs may ultimately be of clinical utility in the treatment of this disease.
Phospholipid interactions of synthetic peptides representing the N-terminus of HIV gp41 (1990)

Peptides representing the N-terminal 23 residues of the surface protein gp41 of LAV1a and LAVmal strains of the human immunodeficiency virus were synthesized and their interactions with phospholipid vesicles studied. The peptides are surface-active and penetrate lipid monolayers composed of negatively charged but not neutral lipids. Similarly, the peptides induce lipid mixing and solute (6-carboxyfluorescein) leakage of negatively charged, but not neutral, vesicles. Circular dichroism and infrared spectroscopy show that at low peptide:lipid ratios (approximately 1:200), the peptides bind to negatively charged vesicles as alpha-helices. At higher peptide:lipid ratios (1:30), a beta conformation is observed for the LAV1a peptide, accompanied by a large increase in light scattering. The LAVmal peptide showed less beta-structure and induced less light scattering. With neutral vesicles, only the beta conformation and a peptide:lipid ratio-dependent increase in vesicle suspension light scattering were observed for both peptides. We hypothesize that the inserted alpha-helical form causes vesicle membrane disruption whereas the surface-bound beta form induces aggregation.
Ruby Laser Treatment of Nevi Spilus and Nevocytic Nevi using Toshiba Model LRT-301A Ruby Laser System (1990)

Forty-two cases with nevi spilus and forty-eight cases with nevocytic nevi have been treated with Toshiba model LRT-301A ruby laser system. The conditions of ruby laser irradiation were output power of 20J/cm2, pulse width of 2ms and 10×10mm square shaped spot using Kaleidoscope. The results of such treatment and the suitable methods of irradiation were reported.1. Pigmented lesions of nevi spilus were able to be easily removed with one application of the ruby laser. But recurrent pigmentations were occured in 76% of the cases within a 6-months follow-up. In the recurrent cases,there were few complete recurrences. The recurrent macules were getting smaller from the margin of lesions, and getting lighter every time two or more additional laser treatment.2. Pigmented lesions of compound nevocytic nevi were not able to be removed completely with one application of the ruby laser. Intact pigmented lesions in the upper dermis were histologically remained. Then the second application of ruby laser in the dermis was required after removing the the coagulated epidermis with the first application. But four or more additional laser treatment at intervals of four-months period were needed to lighten the lesions of compound nevocytic nevi.
Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence. (1995)

In an effort to identify new genes and analyse their expression patterns, 174,472 partial complementary DNA sequences (expressed sequence tags (ESTs)), totalling more than 52 million nucleotides of human DNA sequence, have been generated from 300 cDNA libraries constructed from 37 distinct organs and tissues. These ESTs have been combined with an additional 118,406 ESTs from the database dbEST, for a total of 83 million nucleotides, and treated as a shotgun sequence assembly project. The assembly process yielded 29,599 distinct tentative human consensus (THC) sequences and 58,384 non-overlapping ESTs. Of these 87,983 distinct sequences, 10,214 further characterize previously known genes based on statistically significant similarity to sequences in the available databases; the remainder identify previously unknown genes. Thirty tissues were sampled by over 1,000 ESTs each; only eight genes were matched by ESTs from all 30 tissues, and 227 genes were represented in 20 or more of the tissues sampled with more than 1,000 ESTs. Approximately 40% of identified human genes appear to be associated with basic energy metabolism, cell structure, homeostasis and cell division, 22% with RNA and protein synthesis and processing, and 12% with cell signalling and communication.
Continuous Glucose Monitoring and the Reality of Metabolic Control in Preschool Children With Type 1 Diabetes (2004)

Twice-daily insulin injection rarely achieves control in preschool children with type 1 diabetes. The CGMS is well tolerated by patients and has the advantage of revealing daily glucose trends missed by SMBG.
Development of antibacterial agents of the nalidixic acid type (1977)

Nalidixic acid I (Nogram®, NegGram®) was introduced into therapy in 1963. This compound, which exhibits a good effect on gram-negative bacteria, is employed for treatment of urinary tract infections. At the time of its introduction into therapy nalidixic acid was the representative of a completely new structural type among chemotherapeutic agents. In the succeeding period, intensive work was carried out on the further development of analogous compounds. The present paper will summarize this work, which hitherto has not been reviewed.
Mesenchymal Stem Cells and Endothelial Progenitor Cells Decrease Renal Injury in Experimental Swine Renal Artery Stenosis Through Different Mechanisms (2012)

Endothelial progenitor cells (EPC) and mesenchymal stem cells (MSC) augment tissue repair but possess slightly different properties. How the cellular phenotype affects the efficacy of this approach in renovascular disease is incompletely understood. This study tested the hypothesis that EPC and MSC protect the poststenotic kidney by blunting different disease pathways. Peripheral blood EPC and adipose-derived MSC were expanded and characterized by cell surface markers (e.g., CD34/kinase insert domain receptor, or CD44/CD90). Single-kidney hemodynamics and function were assessed in pigs after 10 weeks of renal artery stenosis (RAS) treated 4 weeks earlier with an intrarenal infusion of vehicle (n = 7), EPC (RAS+EPC) or MSC (RAS+MSC) (both 10 × 10(6), n = 6), and normal controls (n = 7). Kidney disease mechanisms were evaluated ex vivo. The ability of EPC and MSC to attenuate endoplasmic reticulum (ER) stress was also studied in isolated ER and in tubular cells cocultured with EPC and MSC. Glomerular filtration rate in RAS was lower than controls, increased in RAS+EPC, and further improved in RAS+MSC, although both improved renal blood flow similarly. EPC prominently enhanced renal growth factor expression and decreased oxidative stress, while MSC more significantly attenuated renal inflammation, ER stress, and apoptosis. Furthermore, MSC induced a greater decrease in caspase-3 and CHOP expression in cultured tubular cells through mechanisms involving cell contact. EPC and MSC achieve a comparable decrease of kidney injury in RAS by different mechanisms, although MSC elicited slightly superior improvement of renal function. These results support development of cell-based approaches for management of renovascular disease and suggest cell selection based on the underlying pathophysiology of kidney injury.