gebhart/patrepeval huggingface dataset:
WRINKLED Transcription Factors Orchestrate Tissue-Specific Regulation of Fatty Acid Biosynthesis in Arabidopsis (2012)

Acyl lipids are essential constituents of all cells, but acyl chain requirements vary greatly and depend on the cell type considered. This implies a tight regulation of fatty acid production so that supply fits demand. Isolation of the Arabidopsis thaliana WRINKLED1 (WRI1) transcription factor established the importance of transcriptional regulation for modulating the rate of acyl chain production. Here, we report the isolation of two additional regulators of the fatty acid biosynthetic pathway, WRI3 and WRI4, which are closely related to WRI1 and belong to the APETALA2-ethylene-responsive element binding protein family of transcription factors. These three WRIs define a family of regulators capable of triggering sustained rates of acyl chain synthesis. However, expression patterns of the three WRIs differ markedly. Whereas only WRI1 activates fatty acid biosynthesis in seeds for triacylglycerol production, the three WRIs are required in floral tissues to provide acyl chains for cutin biosynthesis and prevent adherence of these developing organs and subsequent semisterility. The targets of these WRIs encode enzymes providing precursors (acyl chain and glycerol backbones) for various lipid biosynthetic pathways, but not the subsequent lipid-assembling enzymes. These results provide insights into the developmental regulation of fatty acid production in plants.
Wavelets on a Bounded Interval (1992)

The aim of this paper is to present two different approaches to the study of multiresolution analysis and wavelets on a bounded interval. Recently, Meyer obtained orthonormal wavelets on a bounded interval by restricting Daubechies’ scaling functions and wavelets to [0, 1] and applying the Gram-Schmidt procedure to orthonormalize the restrictions. Our own approach — presented in the second part of the paper — is based on the semi-orthogonal Chui-Wang spline-wavelets. In this case we no longer have orthogonality in one scale, but there are explicit formulae for these wavelets.
Antibiotic-impregnated cement use in U.S. hospitals (1992)

The results of a survey of the use of antibiotic-impregnated bone cement and cement beads in U.S. hospitals are reported. A random sample of hospitals was selected from all hospitals registered with the American Hospital Association. A questionnaire designed to characterize the extent of use of the products and the degree of pharmacy involvement was mailed to the pharmacy directors at 547 hospitals nationwide. The response rate was 61.7% (336 evaluable returns). Ninety hospitals (26.9%) reported using antibiotic-impregnated bone cement or cement beads. Product use was significantly greater in urban hospitals, hospitals larger than 200 beds, teaching hospitals, and hospitals with pharmaceutical services in the operating rooms. Most facilities using the products were community hospitals. Total hip arthroplasty, total knee arthroplasty, and chronic osteomyelitis were the most common indications for use. Systemic antibiotics were also administered in the great majority of hospitals reporting use of the products. The products were generally used in fewer than one procedure per month. Aminoglycosides and various cephalosporins were the antibiotics most commonly used; most have not been adequately studied for this use. Although nearly all the hospital pharmacies purchased antibiotics for these products, none mixed cement and only two premanufactured antibiotic beads. About one fourth of the hospitals surveyed reported using antibiotic-impregnated bone cement and cement beads, although the total number of patients being treated was small.
Selectivity Mechanisms in Low Pressure Selective Epitaxial Silicon Growth (1994)

Selective silicon processing at 775 and 850°C using an based chemistry was studied. The selectivity of each experimental condition was quantified by measuring the silicon nuclei density/cm2 on large blanket areas of . The morphology of the selective silicon films was examined for texture and hillocks. The roles of water vapor and atomic hydrogen on the microchemistry of an surface was investigated. Thermodynamic modeling of the effect of different atmospheric leak rates on the water vapor and atomic hydrogen concentrations was carried out with the aid of the SOLGAS program. A new understanding of selectivity is proposed. An surface is an interruption of the bulk continuous random network structure and, as such, has a characteristic density of defect sites. The degree of selectivity observed is determined by the type of species which terminate these defect sites, i.e., by the number of defect sites which are not chemically passivated against the adsorption of silicon species. A universal selective silicon processing regime is predicted and good agreement with a wide range of published selective silicon processes is shown.
Terahertz spectroscopy for the assessment of burn injuriesin vivo (2013)

A diagnosis criterion is proposed for noninvasive grading of burn injuries using terahertz radiation. Experimental results are presented from in vivo terahertz time-domain spectroscopy of second- and third-degree wounds, which are obtained in a 72-hour animal study. During this period, the change in the spectroscopic response of the burned tissue is studied. It is shown that terahertz waves are sensitive not only to the postburn formation of interstitial edema, but also to the density of skin structures derived from image processing analysis of histological sections. Based on these preliminary results, it is suggested that the combination of these two effects, as probed by terahertz spectroscopy of the tissue, may ultimately be used to differentiate partial-thickness burns that will naturally heal from those that will require surgical intervention.
A novel RNA‐based adjuvant combines strong immunostimulatory capacities with a favorable safety profile (2014)

Protein‐ and peptide‐based tumor vaccines depend on strong adjuvants to induce potent immune responses. Here, we demonstrated that a recently developed novel adjuvant based on a non‐coding, long‐chain RNA molecule, termed RNAdjuvant ® , profoundly increased immunogenicity of both antigen formats. RNAdjuvant ® induced balanced, long‐lasting immune responses that resulted in a strong anti‐tumor activity. A direct comparison to Poly(I:C) showed superior efficacy of our adjuvant to enhance antigen‐specific multifunctional CD8 + T‐cell responses and mediate anti‐tumor responses induced by peptide derived from HPV‐16 E7 protein in the syngeneic TC‐1 tumor, a murine model of human HPV‐induced cervical cancer. Moreover, the adjuvant was able to induce functional memory responses that mediated complete tumor remission. Despite its remarkable immunostimulatory activity, our RNA‐based adjuvant exhibited an excellent pre‐clinical safety profile. It acted only locally at the injection site where it elicited a transient but strong up‐regulation of pro‐inflammatory and anti‐viral cytokines as well as cytoplasmic RNA sensors without systemic cytokine release. This was followed by the activation of immune cells in the draining lymph nodes. Our data indicate that our RNA‐based adjuvant is a safe and potent immunostimulator that may profoundly improve the efficacy of a variety of cancer vaccines.
A retrospective study of the relationship between serum urate level and recurrent attacks of gouty arthritis: Evidence for reduction of recurrent gouty arthritis with antihyperuricemic therapy (2004)

The data indicate that reduction of serum urate concentrations to 6 mg/dl or lower will eventually result in a reduced frequency or prevention of future gouty attacks.
Modifications of theE.coliLac repressor for expression in eukaryotic cells: effects of nuclear signal sequences on protein activity and nuclear accumulation (1992)

Eukaryotic expression vectors designed to produce E. coli Lac repressor protein targeted to the nucleus of mammalian cells were constructed. These constructions carry the lac repressor gene (lacI) fused at different positions to a nuclear localization sequence (NLS) from either the SV40 large T antigen or the adenovirus E1a. When the NLS's were fused to the lacI gene at the 5' end, the protein produced exhibited tighter repression of beta-galactosidase expression than the unmodified LacI protein. Localization sequences at the extreme 3' end of the gene generally diminished induction by IPTG, while introduction of the SV40 NLS nine base pairs upstream of the 3' end eliminated repressor activity. When either NLS was placed at the 3' end behind a random nine base pair linker, the activity of the LacI protein depended on the sequence of the linker, and in 9 of 10 linkers tested, activity of the protein was adversely affected. The one exception was the fusion protein from p3'ss, which had the NLS at the 3' end of lacI behind the nine base pair linker, AGC AGC CTG (ser-ser-leu). This protein exhibited efficient nuclear accumulation, strong repressor activity and greater sensitivity to IPTG induction. The functional linker from the p3'ss fusion protein extends the leucine zipper heptad repeat located at the C-terminus of the protein. These data support the role of the leucine zipper in tetramer formation and predict that extension of this zipper will further stabilize the protein. This modified lacI gene should be valuable for improved adaptation of the prokaryotic regulatory system to eukaryotic cells.
A Development of Neurofuzzy System for a Conceptual Design of Ship (1998)

The purpose of this paper is to develope a neurofuzzy system for a ship design which can determine efficiently design values e.g. principal dimensions and hull factors in a conceptual design. The neurofuzzy system for a ship design(NeFHull) applies a information about given input-output data to fuzzy theories and deals these fuzzificated values with neural networks, e.g. first, redefines normalized input-output data ad membership functions and then executes these fuzzficated information with backpropagation neural networks. We use a hybrid learning algorithm in the training of neural networks and examine the usefulness of suggested method through mathematical and mechanical examples.
Neural Induction with a Dopaminergic Phenotype from Human Pluripotent Stem Cells Through a Feeder-Free Floating Aggregation Culture (2013)

Pluripotent stem cells are promising potential sources for cell replacement therapy and are useful research tools for exploring disease mechanisms. Neural cells are one of the cell types that have been most efficiently differentiated through several established protocols. This chapter describes the feeder-free floating aggregation culture system for the induction of dopaminergic neurons. This method is simple and highly efficient for the production of dopaminergic neurons. It has several advantages for application in clinical usage in comparison to the other protocols using either feeder cells or Matrigel.
Spontaneous activation and signaling by overexpressed epidermal growth factor receptors in glioblastoma cells (2003)

Overexpressed epidermal growth receptor factor receptors (EGFRs) are thought to contribute to the malignant phenotype of human glioblastomas (GBMs), but the mechanism is not well understood. We found that SKMG-3 cells, a rare GBM cell line that maintains EGFR gene amplification in vitro, produced high levels of EGFR protein. The cells also expressed the related receptors HER2/neu and HER4, but not HER3. Immunoblots and tryptic phosphopeptide maps showed that the SKMG-3 EGFRs were intact and functional and that a subset of these receptors were spontaneously autophosphorylated. EGF treatment stimulated phosphorylation of the EGFRs as well as the downstream effectors Erk, AKT1, stat3 and c-Cbl. Under minimal growth conditions, the unstimulated SKMG-3 cells contained constitutively phosphorylated Erk and AKTI but no detectable stat3 DNA-binding complexes. The EGFR kinase inhibitor PD158780 reduced the constitutive phosphorylation of the receptor and Erk but not that of AKT1. In contrast, inhibition of phosphatidylinositol-3-kinase (PI3K) blocked the constitutive phosphorylation of Erk and AKT-1 but not the EGFR. We conclude that the SKMG-3 cells represent the subset of GBMs with amplified EGFR genes that overexpress intact receptors. The results also suggest that in some GBMs, signals from overexpressed EGFRs contribute to the constitutive phosphorylation of Erk, but these signals may not required for the constitutive activation of PI3K or AKT1.
Einleitung (1992)

Unter der „Philosophie“ einer Programmiersprache versteht man die Ideen und Intentionen, die zu ihrer Entstehung geführt haben und Grundlage ihrer Definition sind.
PP.11.05 (2015)

Objective: Plasma renin activity (PRA) is accepted as a marker for increased risk of cardiovascular diseases. We examined whether PRA is a risk factor left ventricular structure abnormal in hypertension patients of Han Chinese.Design and method: This cross-sectional study included a total of 879 consecutive Han patients diagnosed essential hypertension who were hospitalization at Department of Hypertension in People's Hospital of Xinjiang Uygur Autonomous Region in China between 2009 and 2013, subjects aged from 30 years to 60 years. All participants who had not taken antihypertensive drugs at least four weeks, or stopped the drug to effect hormone, such as contraception pills, diuretic dihydropyridines calcium antagonist, beta blockers, angiotensin converting enzyme inhibitors and angiotensin II receptor inhibitor. 24-h ambulatory blood pressure monitoring, echocardiography, plasma renin activity and plasma aldosterone were also performed. LV mass index (LVMI) was calculated according to the method of Devereux and Reichek. LV hypertrophy (LVH) was defined as LVMI>=125 g/m2 in men and >=110 g/m2 in women. According to LVMI and RWT, concentric remodeling (CCR), a normal LVMI and RWT>=0.45; concentric hypertrophy (CCH), RWT<0.45 and LVH; eccentric hypertrophy (ECH) RWT>=0.45 and LVH. According to the plasma renin activity lever, participants were classified three renin categories, as follows: low renin, <0.56ng/ml/h (30% of the sample); medium renin, 0.56 to 4.24ng/ml/h (60% of the sample); or high renin,>4.24ng/ml/h(10% of the sample) Results: As compared with low-renin and medium-renin groups, high-renin group had high mean 24-h mean arterial pressure. High-renin group show a high prevalence of LVMI and concentric hypertrophy, eccentric hypertrophy than low and medium renin groups adjusting for sex, age, BMI, and mean 24 hours MAP. Regression models showed that high-renin group showed significantly increased 3.033 fold risk of left ventricular hypertrophy (p < 0.001), compared to low-renin group, as potential confounders for age, gender, BMI, serum creatinine, high-sensitivity C-reactive protein, triglyceride,duration. Conclusions: These results raise the hypothesis that high plasma renin activity may have a deleterious effort on the development of LV structure in essential hypertension.
Molecular Genetics of Alzheimer's Disease (1993)

The part that genetics plays in the origin of Alzheimer's disease (AD) is a complex problem that is only now, in the last few years, beginning to be understood. Progress in the study of the epidemiology of AD, discovery of multiple AD loci, and interpreting how mutations affect and produce the AD phenotype have been the initial keys to unlocking the mysteries of this disease. We now know of the existence of at least three AD loci on chromosomes 14, 19, and 21 and are beginning to understand the role that one of these loci, APP, and its mutations plays in the progression of AD. On future studies using animal modeling and the positional cloning of the other AD loci, a definite model for AD should become evident within the next few years.
Evaluation of mixed‐conducting lanthanum‐strontium‐cobaltite ceramic membrane for oxygen separation (2009)

Abstract In this study, La 0.4 Sr 0.6 CoO 3‐δ (LSC) oxide was synthesized via an EDTA‐citrate complexing process and its application as a mixed‐conducting ceramic membrane for oxygen separation was systematically investigated. The phase structure of the powder and microstructure of the membrane were characterized by XRD and SEM, respectively. The optimum condition for membrane sintering was developed based on SEM and four‐probe DC electrical conductivity characterizations. The oxygen permeation fluxes at various temperatures and oxygen partial pressure gradients were measured by gas chromatography method. Fundamental equations of oxygen permeation and transport resistance through mixed conducting membrane were developed. The oxygen bulk diffusion coefficient (D v ) and surface exchange coefficient (K ex ) for LSC membrane were derived by model regression. The importance of surface exchange kinetics at each side of the membrane on oxygen permeation flux under different oxygen partial pressure gradients and temperatures were quantitatively distinguished from the oxygen bulk diffusion. The maximum oxygen flux achieved based on 1.6‐mm‐thick La 0.4 Sr 0.6 CoO 3‐δ membrane was ∼4.0 × 10 −7 mol cm −2 s −1 at 950°C. However, calculation results show theoretical oxygen fluxes as high as 2.98 × 10 −5 mol cm −2 s −1 through a 5‐μm‐thick LSC membrane with ideal surface modification when operating at 950°C for air separation. © 2009 American Institute of Chemical Engineers AIChE J, 2009
Cell-Based Regeneration of Intervertebral Disc Defects: Review and Concepts (2009)

During the last century low back pain has emerged as a widespread disease often caused by intervertebral disc degeneration (IDD). IDD is a complex problem in which a variety of causes play a role. As IDD causes high costs, corporate interest has led to a number of therapies being developed. Today, these therapies focus not only on minimizing the pain caused by this disease but also on restoring intervertebral disc function. These approaches are often biological and aim to stimulate the regeneration of the intervertebral disc by injection of activator proteins, biomaterials, different cell types or complex cell matrix composites. Genetic engineering of disc cells and in vitro tissue engineering also offer a possibility for curing IDD. This article gives an overview of these concepts.
Ceramide does not mediate the effect of tumour necrosis factor α on superoxide generation in human neutrophils (1994)

The effect of tumour necrosis factor alpha (TNF alpha) on superoxide generation in human neutrophils was investigated using the Nitro Blue Tetrazolium reduction assay. TNF alpha stimulated superoxide generation in a time- and concentration-dependent fashion. The maximally effective concentration of TNF alpha for superoxide generation was 10 nM and maximal response was obtained after 15-20 min. The monoclonal antibody (mAb), utr-1, which was raised against the 75 kDa receptor and behaves as an antagonist, had no effect on superoxide generation, but partially inhibited the response to TNF alpha. mAb htr-9, which was raised against the 55 kDa receptor and behaves as an agonist, mimicked the effect of TNF alpha, but with a lower maximal response. As it has been reported that ceramide might act as a second messenger to mediate many of the effects of TNF alpha, the effects of exogenous sphingomyelinase and the cell-permeable ceramide analogue, C2- ceramide, on production of superoxide anions, induction of priming in response to formylmethionyl-leucyl-phenylalanine, and cell-shape change were examined. Neither sphingomyelinase nor C2-ceramide mimicked the effect of TNF alpha. Ceramide is converted into ceramide 1-phosphate by ceramide kinase and we have measured levels of this metabolite to clarify the effect of TNF alpha on sphingomyelinase activity in neutrophils. Although exogenous sphingomyelinase increased the amount of ceramide 1-phosphate in a time-dependent manner, and C2-ceramide was rapidly converted into C2-ceramide phosphate, TNF alpha had no effect on the level of ceramide 1-phosphate. These results suggest that TNF alpha stimulates superoxide generation through both the 55 kDa and 75 kDa receptors, but that ceramide does not act as an intracellular mediator for TNF alpha in human neutrophils.
Collaborative gaming in augmented reality (1998)

Article Collaborative gaming in augmented reality Share on Authors: Zsolt Szalavári Institute of Computer Graphics, Vienna University of Technology, Karlsplatz 13/186/2, A-1040 Vienna, Austria Institute of Computer Graphics, Vienna University of Technology, Karlsplatz 13/186/2, A-1040 Vienna, AustriaView Profile , Erik Eckstein Institute of Computer Graphics, Vienna University of Technology, Karlsplatz 13/186/2, A-1040 Vienna, Austria Institute of Computer Graphics, Vienna University of Technology, Karlsplatz 13/186/2, A-1040 Vienna, AustriaView Profile , Michael Gervautz Institute of Computer Graphics, Vienna University of Technology, Karlsplatz 13/186/2, A-1040 Vienna, Austria Institute of Computer Graphics, Vienna University of Technology, Karlsplatz 13/186/2, A-1040 Vienna, AustriaView Profile Authors Info & Claims VRST '98: Proceedings of the ACM symposium on Virtual reality software and technologyNovember 1998 Pages 195–204https://doi.org/10.1145/293701.293740Published:02 November 1998 51citation1,852DownloadsMetricsTotal Citations51Total Downloads1,852Last 12 Months62Last 6 weeks4 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Poly(2,5-ethynylenethiophenediylethynylenes), related heteroaromatic analogs, and poly(thieno[3,2-b]thiophenes): synthesis and thermal and electrical properties (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPoly(2,5-ethynylenethiophenediylethynylenes), related heteroaromatic analogs, and poly(thieno[3,2-b]thiophenes): synthesis and thermal and electrical propertiesDenise R. Rutherford, J. K. Stille, C. Michael Elliott, and Veronica R. ReichertCite this: Macromolecules 1992, 25, 9, 2294–2306Publication Date (Print):April 27, 1992Publication History Published online1 May 2002Published inissue 27 April 1992https://pubs.acs.org/doi/10.1021/ma00035a003https://doi.org/10.1021/ma00035a003research-articleACS PublicationsRequest reuse permissionsArticle Views1131Altmetric-Citations74LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Immunology of Reproduction (1993)

This comprehensive review describes the latest developments in reproductive immunology. The main focus is on the broad areas of infertility, pregnancy loss, and immunocontraception. Specific topics covered within infertility include the significance of antisperm antibodies and the sperm antigens involved, cell-mediated immunity, pathogenesis of endometriosis, and reproductive immunology of HIV infection. The section on pregnancy loss describes the role that immunology plays during pregnancy, both in foetal survival as well as in foetal demise.