gebhart/patrepeval huggingface dataset:
Applications to Analytical Chemistry (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTApplications to Analytical ChemistryMatthias OttoCite this: Anal. Chem. 1990, 62, 14, 797A–802APublication Date (Print):July 15, 1990Publication History Published online30 May 2012Published inissue 15 July 1990https://doi.org/10.1021/ac00213a735RIGHTS & PERMISSIONSArticle Views30Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (6 MB) Get e-Alerts Get e-Alerts
Tyr-716 in the Platelet-Derived Growth Factor β-Receptor Kinase Insert Is Involved in GRB2 Binding and Ras Activation (1994)

Ligand stimulation of the platelet-derived growth factor (PDGF) beta-receptor leads to activation of its intrinsic tyrosine kinase and autophosphorylation of the intracellular part of the receptor. The autophosphorylated tyrosine residues mediate interactions with downstream signal transduction molecules and thereby initiate different signalling pathways. A pathway leading to activation of the GTP-binding protein Ras involves the adaptor molecule GRB2. Here we show that Tyr-716, a novel autophosphorylation site in the PDGF beta-receptor kinase insert, mediates direct binding of GRB2 in vitro and in vivo. In a panel of mutant PDGF beta-receptors, in which Tyr-716 and the previously known autophosphorylation sites were individually mutated, only PDGFR beta Y716F failed to bind GRB2. Furthermore, a synthetic phosphorylated peptide containing Tyr-716 bound GRB2, and this peptide specifically interrupted the interaction between GRB2 and the wild-type receptor. In addition, the Y716(P) peptide significantly decreased the amount of GTP bound to Ras in response to PDGF in permeabilized fibroblasts as well as in porcine aortic endothelial cells expressing transfected PDGF beta-receptors. The mutant PDGFR beta Y716F still mediated activation of mitogen-activated protein kinases and an increased DNA synthesis in response to PDGF, indicating that multiple signal transduction pathways transduce mitogenic signals from the activated PDGF beta-receptor.
Knowledge Mapping for Facility Scope Modeling (1993)

This paper demonstrated how knowledge mapping can be applied to develop standard scope templates for facility scope management. We first develop several examples to illustrate this idea. Properties of the knowledge-map representation as a facility scope modeling means are discussed through these examples. We then introduce a contingency evaluation example to show the application of facility scope models developed from the knowledge-map representation. Finally, we conclude that the knowledge-map representation of facility scope models is an important tool for the development of intelligent aids for facility scope management.
High-speed dynamic logic styles for scaled-down CMOS and MTCMOS technologies (2000)

A new high-speed domino circuit, called HS-Domino is developed. HS-Domino resolves the trade-off between performance and noise margins in conventional CD-Domino logic while dissipating low dynamic power with minimal area overhead. A dual-threshold (MTCMOS) implementation of HS-Domino and DDCVS logic is also devised. This implementation achieves low leakage values during standby, while maintaining high performance and low dynamic power during the active mode.
Composite Cores: Pushing Heterogeneity Into a Core (2012)

Heterogeneous multicore systems -- comprised of multiple cores with varying capabilities, performance, and energy characteristics -- have emerged as a promising approach to increasing energy efficiency. Such systems reduce energy consumption by identifying phase changes in an application and migrating execution to the most efficient core that meets its current performance requirements. However, due to the overhead of switching between cores, migration opportunities are limited to coarse-grained phases (hundreds of millions of instructions), reducing the potential to exploit energy efficient cores. We propose Composite Cores, an architecture that reduces switching overheads by bringing the notion of heterogeneity within a single core. The proposed architecture pairs big and little compute μEngines that together can achieve high performance and energy efficiency. By sharing much of the architectural state between the μEngines, the switching overhead can be reduced to near zero, enabling fine-grained switching and increasing the opportunities to utilize the little μEngine without sacrificing performance. An intelligent controller switches between the μEngines to maximize energy efficiency while constraining performance loss to a configurable bound. We evaluate Composite Cores using cycle accurate micro architectural simulations and a detailed power model. Results show that, on average, the controller is able to map 25% of the execution to the little μEngine, achieving an 18% energy savings while limiting performance loss to 5%.
Acute kidney dysfunction and the critically ill. (2006)

Given the devastating effect that acute kidney dysfunction (AKD) has on the clinical course and outcome of critically ill patients, it is incumbent on every intensive care provider to understand the causes and effects of AKD. AKD is common and costly and even mild forms warrant attention. Promising new therapies are being explored. But even after they arrive, attention to first principles of avoiding further injury from volume depletion, hypotension and nephrotoxins will be of the primary concern of Intensive Care Unit (ICU) team. For established AKD, more and possibly sooner renal replacement therapy is likely to be better than less and later.
Combination of Chromium and Biotin Improves Coronary Risk Factors in Hypercholesterolemic Type 2 Diabetes Mellitus: A Placebo‐Controlled, Double‐Blind Randomized Clinical Trial (2007)

Dyslipidemia, often found in type 2 diabetes mellitus (T2DM) patients, plays an important role in the progression of cardiometabolic syndrome. Two essential nutrients, chromium and biotin, may maintain optimal glycemic control. The authors report here a randomized, double-blind placebo-controlled trial (N=348; chromium picolinate and biotin combination [CPB]: 226, placebo: 122; T2DM participants with hemoglobin A1c [HbA1c] >or=7%) evaluating the effects of CPB on lipid and lipoprotein levels. Participants were randomly assigned (2:1 ratio) to receive either CPB (600 microg chromium as chromium picolinate and 2 mg biotin) or a matching placebo once daily for 90 days. Statistical analyses were conducted in all eligible participants. Subsequent supplemental analyses were performed in T2DM participants with hypercholesterolemia (HC) and in those using stable doses of statins. In the primary analysis, CPB lowered HbA1c (P<.05) and glucose (P<.02) significantly compared with the placebo group. No significant changes were observed in other lipid levels. In participants with HC and T2DM, significant changes in total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and atherogenic index were observed in the CPB group (P<.05). Significant decreases in LDL-C, total cholesterol, HbA1c , and very low-density cholesterol levels (P<.05) were observed in the CPB group taking statins. CPB treatment was well tolerated with no adverse effects, dissimilar from those associated with placebo. These data suggest that intervention with CPB improves cardiometabolic risk factors.
Low Nucleotide Diversity in Chimpanzees and Bonobos (2003)

Comparison of the levels of nucleotide diversity in humans and apes may provide much insight into the mechanisms of maintenance of DNA polymorphism and the demographic history of these organisms. In the past, abundant mitochondrial DNA (mtDNA) polymorphism data indicated that nucleotide diversity (pi) is more than threefold higher in chimpanzees than in humans. Furthermore, it has recently been claimed, on the basis of limited data, that this is also true for nuclear DNA. In this study we sequenced 50 noncoding, nonrepetitive DNA segments randomly chosen from the nuclear genome in 9 bonobos and 17 chimpanzees. Surprisingly, the pi value for bonobos is only 0.078%, even somewhat lower than that (0.088%) for humans for the same 50 segments. The pi values are 0.092, 0.130, and 0.082% for East, Central, and West African chimpanzees, respectively, and 0.132% for all chimpanzees. These values are similar to or at most only 1.5 times higher than that for humans. The much larger difference in mtDNA diversity than in nuclear DNA diversity between humans and chimpanzees is puzzling. We speculate that it is due mainly to a reduction in effective population size (N(e)) in the human lineage after the human-chimpanzee divergence, because a reduction in N(e) has a stronger effect on mtDNA diversity than on nuclear DNA diversity. Sequence data from this article have been deposited with the GenBank Data libraries under accession nos. AY 275957-AY 277244.
[Application of 2d NMR techniques in the structure determination of ganosporelactone A and B]. (1991)

Structure and stereochemistry of ganosporelactone A and B isolated from the spores of Ganoderma lucidum have been elucidated by the use of 1H-1H COSY, 1H-13C COSY, 1H-13C COLOC and NOESY 2D NMR spectroscopy. Ganosporelactone A and B are two novel pentacyclic triterpenoids which may be biogenetically derived from lanostane skeleton through the construction of C16 and C23 bond.
A dicentric iso (Y) chromosome in an infertile male. (1985)

A dicentric Y chromosome was detected in a 30-year-old azoospermic male patient who was found to be mosaic for 45,X/46,X,dic Y(qter----p11::p11----qter). The dicentric iso (Y) chromosome was identified conclusively with C-banding, G-banding and Q-banding techniques. The relationship of structural abnormalities of the Y chromosome and azoospermia is discussed.
ACPYPE - AnteChamber PYthon Parser interfacE (2012)

ACPYPE is a tool that simplifies the automatic generation of topology and parameters in different formats for different molecular mechanics programmes, including calculation of partial charges, while being object oriented for integration with other applications.
Insulinotropic action of β-l-glucose pentaacetate (1998)

The metabolism of beta-L-glucose pentaacetate and its interference with the catabolism of L-[U-14C]glutamine, [U-14C]palmitate, D-[U-14C]glucose, and D-[5-3H]glucose were examined in rat pancreatic islets. Likewise, attention was paid to the effects of this ester on the biosynthesis of islet peptides, the release of insulin from incubated or perifused islets, the functional behavior of individual B cells examined in a reverse hemolytic plaque assay of insulin secretion, adenylate cyclase activity in a membrane-enriched islet subcellular fraction, cAMP production by intact islets, tritiated inositol phosphate production by islets preincubated with myo-[2-3H]inositol, islet cell intracellular pH, 86Rb and 45Ca efflux from prelabeled perifused islets, and electrical activity in single isolated B cells. The results of these experiments were interpreted to indicate that the insulinotropic action of beta-L-glucose pentaacetate is not attributable to any nutritional value of the ester but, instead, appears to result from a direct effect of the ester itself on a yet unidentified receptor system, resulting in a decrease in K+ conductance, plasma membrane depolarization, and induction of electrical activity.
Scalable Distributed Stream Join Processing (2015)

Efficient and scalable stream joins play an important role in performing real-time analytics for many cloud applications. However, like in conventional database processing, online theta-joins over data streams are computationally expensive and moreover, being memory-based processing, they impose high memory requirement on the system. In this paper, we propose a novel stream join model, called join-biclique, which organizes a large cluster as a complete bipartite graph. Join-biclique has several strengths over state-of-the-art techniques, including memory-efficiency, elasticity and scalability. These features are essential for building efficient and scalable streaming systems. Based on join-biclique, we develop a scalable distributed stream join system, BiStream, over a large-scale commodity cluster. Specifically, BiStream is designed to support efficient full-history joins, window-based joins and online data aggregation. BiStream also supports adaptive resource management to dynamically scale out and down the system according to its application workloads. We provide both theoretical cost analysis and extensive experimental evaluations to evaluate the efficiency, elasticity and scalability of BiStream.
Cd8 T Cell Detection of Bacterial Infection (2001)

Bacteria remain a major cause of human disease and a formidable foe of the mammalian immune system. Bacter-ial entry into normally sterile compartments has long been recognized as a potential calamity for the mammalian host, in the most extreme circumstances resulting in circulatory collapse and multiorgan failure. In contrast to viruses, most bacteria are self-sufficient, producing their own lipid membranes, cell walls, nucleic acids, and proteins. Although many synthetic pathways used by bacteria and mammals are similar, others are subtly different and some are completely distinct. The mammalian immune system has evolved an array of mechanisms to recognize and respond to these distinct bacterial products upon infection. While it has been appreciated for over a century that the mammalian immune system uses receptors to specifically recognize bacterial products and initiate inflammatory defenses upon infection, the last decade has witnessed the molecular characterization of a number of remarkable pathways of immune defense against bacteria.
Human immunoglobulin VH and D segments on chromosomes 15q11.2 and 16p11.2 (1994)

In addition to the major human immunoglobulin heavy-chain locus on chromosome 14q32.3, VH and D segments are known to be present on chromosomes 15 and 16. We have now amplified and sequenced 24 such VH segments from somatic cell hybrids and have assigned them to 15q11.2 and 16p11.2 using cosmid and yeast artificial chromosome clones. In addition, we have located a cluster of D segments on 15q11.2, previously thought to be located on 14q32.3. We propose that the segments on chromosome 16 arose by an interchromosomal duplication and identify the corresponding region on chromosome 14. Taken together with the completion of a map of the human VH locus on 14q32.3, the total number of VH segments now identified is 117. We can now account for most, if not all human germ-line VH segments.
Lienotoxicity Of Bordetella Pertussis In Mice (1971)

SUMMARY A highly toxic fraction causing dermonecrosis in guinea-pigs and a lethal effect in mice was obtained from Bordetella pertussis phase-1 organisms. Intravenous injection of this toxic fraction into mice produced a considerable reduction in spleen weight within 7 days. The histological findings indicate that B. pertussis causes an atrophy of the spleen. The term “lienotoxicity” is proposed to designate this toxic phenomenon. Whether dermonecrosis, lethality and lienotoxicity are caused by three different agents contained in the preparation studied has not been determined.
A re-appraisal of lactose intolerance (2008)

The terminology relating to lactose intolerance is confusing for clinicians and researchers. Clinicians need to ensure that these problematic terms do not cause diagnostic mistakes and inappropriate treatment. Researchers should be aware of inconsistent terminology in studies and resultant problems with the interpretation of results.
Thermal Behavior of Alkylsiloxane Self-Assembled Monolayers on the Oxidized Si(100) Surface (1997)

The thermal behavior of alkyltrichlorosilane (CH3(CH2)n-1SiCl3) based self-assembled monolayers on the oxidized Si(100) surface has been examined under ultrahigh vacuum conditions for n = 4, 8, and 18. Using high-resolution electron energy loss spectroscopy and contact angle analysis, it is found that the monolayers are stable in vacuum up to about 740 K independent of chain length. Above 740 K the chains begin to decompose through C−C bond cleavage, resulting in the desorption of hydrocarbon fragments. Following this initial desorption step, methyl groups attached to silicon atoms are observed. The siloxane head groups remain on the surface following decomposition of the monolayers until about 1100 K.
Preparation of Ca2+ selective sorbents by molecular imprinting using polymerisable ionophores (1991)

Molecular imprints against calcium and magnesium ions, respectively, were prepared in divinylbenzene-based polymers. A vinylic, Ca2+-selective, neutral ionophore N,N′-dimethyl-N,N′-bis-(4-vinylphenyl)-3-oxapentanediamide was synthesized and used as the ion-complexing monomer. In order to investigate the ion-complexing abilities of this neutral ionophore, this compound was tested as a neutral carrier in the plasticised PVC membrane of an ion-selective electrode. Electrode response and selectivity coefficients recorded for the ionophore proved to be equivalent to the values recorded for similar parent diglycolic acid amides. In analogy with other 3-oxapentanediamide derivatives, the ionophore is expected to form complexes with calcium and magnesium ions with a molar ratio, ligand to metal ion, of 3 : 1 and 2 : 1, respectively. Therefore the metal ion, added to the polymerisation mixture, was expected to act as a template for the ionophore during the polymerisation. The resulting polymers were analysed for their ability to extract calcium ions from methanolic water. The polymers prepared against calcium and magnesium ions were found to bind calcium ions with 6- and 1.7-times lower Kdiss-values, respectively, when compared with reference polymers prepared in the absence of metal ions. The increased binding strength is attributed to the spatial arrangement of ionophore units in the resulting polymers by the template ions during the polymerisation. In addition, the number of binding sites for calcium ions, determined for the respective polymer preparation, fitted well with theoretical values calculated from the stoichiometry of complexation of the ionophore by calcium and magnesium ions, respectively.
Healable, Reconfigurable, Reprocessable Thermoset Shape Memory Polymer with Highly Tunable Topological Rearrangement Kinetics (2017)

The unique capability of topological rearrangement for dynamic covalent polymer networks has enabled various unusual properties (self-healing, solid-state plasticity, and reprocessability) that are not found in conventional thermosets. Achieving these properties in one network in a synergetic fashion can open up new opportunities for shape memory polymer. To accomplish such a goal, the freedom to tune topological rearrangement kinetics is critical. This is, however, challenging to achieve. In this work, two sets of dynamic bonds (urethane and hindered urea) are incorporated into a hybrid network for synthesizing shape memory poly(urea-urethane). By changing the bond ratio, networks with highly tunable topological rearrangement kinetics are obtained. Combining self-healing, solid-state plasticity, and reprocessability in one such shape memory network leads to unusual versatility in its shape-shifting performance.
Tumor-infiltrating lymphocytes for adoptive cell therapy: recent advances, challenges, and future directions (2022)

There are many ongoing areas of research focusing on improving clinical efficacy and optimizing TIL-based ACT. Many strategies have shown a great potential, particularly strategies advancing TIL efficacy (such as increasing and harnessing <i>ex vivo</i> the sub-population of tumor-reactive TILs) and manufacturing processes. Novel approaches can help overcome current limitations and potentially result in TIL-based ACT entering the mainstream of cancer therapy across tumor types.
Polymeric Systems as Nanodevices for siRNA Delivery (2013)

The delivery of small interfering RNAs (siRNAs) is a promising approach to silencing gene expression aimed at treating infections, cancer, neurodegenerative diseases and various other disorders. Recent progress in this area has been achieved with nanodevices possessing multiple properties and assembled with new, biodegradable, synthetic polymers and polysaccharides. Different synthetic routes and multiple strategies, such as multilayer systems and stimuliresponsive polymers, have been developed to attain high efficiencies. This review covers the most important, promising and successful approaches to improve siRNA delivery. It is a concise report on multiple strategies employed, including cell-specific delivery coupling ligands or antibodies with nanodevices to improve siRNA efficiency and specificity.
Multilocus enzyme electrophoresis analysis of the Mycobacterium avium complex and other mycobacteria (1991)

Multilocus enzyme electrophoresis analysis was used to evaluate the Mycobacterium avium complex (MAC), M. paratuberculosis, and nine other mycobacterial species. The average number of alleles per locus was 2.8 for the 35 MAC and 2 M. paratuberculosis strains which represented 24 electrophoretic types (ETs) and two distinct groups. The M. avium group was resolved into 17 ETs and contained the M. paratuberculosis ET. The M. intracellulare group consisted of six ETs. There was complete agreement between Gen-Probe identification and group placement by multilocus enzyme electrophoresis. The mean genetic diversity per locus for the 24 MAC ETs was 0.38. This procedure subdivided some serovars and, if implemented, should prove to be a powerful epidemiologic tool for the MAC. Eleven additional ETs were formed after the data for the other mycobacterial species were pooled with those for the MAC.
Mechanoactive Scaffold Induces Tendon Remodeling and Expression of Fibrocartilage Markers (2008)

Biological fixation of soft tissue-based grafts for anterior cruciate ligament (ACL) reconstruction poses a major clinical challenge. The ACL integrates with subchondral bone through a fibrocartilage enthesis, which serves to minimize stress concentrations and enables load transfer between two distinct tissue types. Functional integration thus requires the reestablishment of this fibrocartilage interface on reconstructed ACL grafts. We designed and characterized a novel mechanoactive scaffold based on a composite of poly-alpha-hydroxyester nanofibers and sintered microspheres; we then used the scaffold to test the hypothesis that scaffold-induced compression of tendon grafts would result in matrix remodeling and the expression of fibrocartilage interface-related markers. Histology coupled with confocal microscopy and biochemical assays were used to evaluate the effects of scaffold-induced compression on tendon matrix collagen distribution, cellularity, proteoglycan content, and gene expression over a 2-week period. Scaffold contraction resulted in over 15% compression of the patellar tendon graft and upregulated the expression of fibrocartilage-related markers such as Type II collagen, aggrecan, and transforming growth factor-beta3 (TGF-beta3). Additionally, proteoglycan content was higher in the compressed tendon group after 1 day. The data suggest the potential of a mechanoactive scaffold to promote the formation of an anatomic fibrocartilage enthesis on tendon-based ACL reconstruction grafts.