gebhart/patrepeval huggingface dataset:
Highly Efficient Red Phosphorescent Dopants in Organic Light‐Emitting Devices (2011)

Highly efficient red phosphorescent dopants in organic light-emitting devices have been explored by using a cyclometalated iridium complex with a fully methylated phenyl ring and a quinoline ring as well as a sterically crowded ancillary ligand. The red phosphorescent devices with these dopants give extremely high external quantum efficiencies.
A Human Monoclonal Antibody to HIV-1 gp41 with Neutralizing Activity Against Diverse Laboratory Isolates (1996)

A potential component that may be useful for passive immunotherapy for HIV-1 is human monoclonal antibodies (HumAbs) possessing potent anti-HIV-1 activity that is directed against conserved regions of the envelope glycoprotein. Such antibodies would, in principle, have the ability to neutralize diverse isolates of HIV-1. To develop such reagents, hybridomas were derived by initial Epstein Barr virus transformation of peripheral blood mononuclear cells (PBMCs) from an asymptomatic HIV-1 seropositive donor followed by fusion with heteromyelomas, and secreted anti-HIV-1 antibodies were further characterized. The specificity of one HumAb, designated as clone 3, was determined by enzyme-linked immunosorbent assay (ELISA) and Western blotting analyses that indicated reactivity to the transmembrane envelope glyco-protein gp41. Synthetic pentadecapeptides overlapping by 10 amino acids were utilized for epitope mapping of clone 3; a decapeptide GCSGKLICTT in the transmembrane gp41 was identified as the epitope. Clone 3 bound to SupT1 cells infected with HTLV-IIIB in fluorescent activated cell sorting analysis. In addition, in vitro biological assays demonstrated that clone 3 possessed neutralization reactivity against diverse laboratory isolates as well as an AZT-resistant isolate. Therefore, clone 3 reactivity defines a conserved neutralizable site on the HIV-1 transmembrane glycoprotein. Clone 3 and the conserved immunogenic epitope on gp41 could be useful in passive and active immunotherapy for the acquired immunodeficiency syndrome (AIDS).
Production of spherical powders on the basis of group IV metals for additive manufacturing (2017)

The method of production of group IV powders for additive manufacturing is presented. Powders are produced in the hydrogenation-dehydrogenation process followed by plasma spheroidization of prepared polygonal powders. The dependence of composition of hydrides on amount of absorbed hydrogen is established for the group IV metals; it allows optimizing the technological parameters of processes for hydrogenation and grinding. It is shown that spherical powders of titanium, Ti–6Al–4V, and Zr–10% Ti of mass fractions of 40–70 μm and not less than 40 μm with calculated spheroidization level achieving 96% may be produced in Ar + 8 vol % H2 thermal plasma flow generated by an arc-jet plasma generator. The average roundness factor is 1.01.
Four years experience with dura mater cardiac valves. (1977)

The clinical use of homologous dura mater cardiac valves was begun in January 1971. Between January 1971 and May 1975, 751 patients received 849 dura mater valves: 428 mitral, 365 aortic, 52 tricuspid and 4 pulmonary. The hospital mortality observed in this series was 13.6% and late mortality was 2.5%. The main causes of death were low-output syndrome in the immediate post-operative period or cardiac failure in the late post-operative period, due to myocardial impairment. Paravalvular leaks and errors in the manufacture of the valve were the main causes of regurgitation both in the immediate and late post-operative period. The incidence of thromboembolism, bacterial and fungal endocarditis was: 0.79%, 0.39%, 0.53% respectively. The results obtained with dura mater valve in a period of four years are satisfactory from the clinical and hemodynamic points of view.
Designing visualizations of social activity (2003)

In this paper, we describe a set of claims that have evolved from our work in designing visual representations of groups in online environments. We argue that these claims can serve as a good starting point for design work, and can drive critical discussions amongst design stakeholders.
A CRITICAL REVIEW ON THE CONSENSUS AROUND THE “WESTPHALIAN SYSTEM” (2012)

The Thirty Years’ War, which devastated Europe between 1618 and 1648, was a complex conflict: it was a religious war; it involved the main powers of the time, dynasty rivalries and rebellions from princes against the Emperor of the Holy Roman-German Empire. There is a consensus in viewing the Peace of Westphalia, which ended the war, as a decisive moment in the history of international relations and the majority of authors considers it the starting point of the modern State-Nation system, sovereign states which have jurisdiction over a territory, which were usually secular and related to one another according to the principle of the balance of power. A critical review of this consensus leads to question each of the above mentioned topics and conclude that the common interpretation has retrospectively transposed political processes which took place only later. In fact, it is likely that, in the 17th century, the pre-modern princely State is still dominant, which will then lead to the modern State-Nation system, a consequence of the emergence of industrial society and nationalism. One may even consider that the Peace of Westphalia delayed the constitution of national States, as far as Germany is concerned. Therefore, to use the terms “Westphalian state” and “Westphalian system” seems rather unadvisable.
NetVLAD: CNN Architecture for Weakly Supervised Place Recognition (2016)

We tackle the problem of large scale visual place recognition, where the task is to quickly and accurately recognize the location of a given query photograph. We present the following four principal contributions. First, we develop a convolutional neural network (CNN) architecture that is trainable in an end-to-end manner directly for the place recognition task. The main component of this architecture, NetVLAD, is a new generalized VLAD layer, inspired by the "Vector of Locally Aggregated Descriptors" image representation commonly used in image retrieval. The layer is readily pluggable into any CNN architecture and amenable to training via backpropagation. Second, we create a new weakly supervised ranking loss, which enables end-to-end learning of the architecture's parameters from images depicting the same places over time downloaded from Google Street View Time Machine. Third, we develop an efficient training procedure which can be applied on very large-scale weakly labelled tasks. Finally, we show that the proposed architecture and training procedure significantly outperform non-learnt image representations and off-the-shelf CNN descriptors on challenging place recognition and image retrieval benchmarks.
Effects of Inhalational Anesthetics on Biochemical Events in Growing Neuronal Tips (1993)

Considering the crucial role of growth cones in the establishment of the neuronal network, the interruption of signal transduction in the growth cone at a time that is critical in directing the neurite extension may evoke a long-lasting alteration in the neural network. Therefore, the effect of inhalational anesthetics on the growth cone enzyme observed in this study may have a major role in the mechanism that induces morphologic or behavioral neuroabnormalities in later life.
Exploiting Circuit‐Specific Spread of Pseudorabies Virus in the Central Nervous System: Insights to Pathogenesis and Circuit Tracers (2002)

The neurotropic alpha-herpesviruses are common mammalian pathogens that invade the peripheral and central nervous system of their hosts. Their ability to invade and spread in the nervous system in a directional manner has been exploited to develop them as neuronal circuit tracers. Tracing viruses spread among synaptically connected neurons and, by assaying brain sections for viral antigen or reporter genes expressed from the viruses, chains of synaptically connected neurons can be visualized. Virulent field strains generally are not good tracers, but some attenuated strains perform well. Live attenuated vaccine strains of pseudorabies virus (PRV), such as PRV Bartha, are among the most popular virus circuit tracers. It may be counterintuitive that attenuation results in improved neural tracing that requires extensive replication and spread in the brain. This report summarizes two lines of experiments directed to resolving this apparent paradox and introduces a new paradigm for tracing viruses.
Bacterial CpG-DNA activates dendritic cellsin vivo: T helper cell-independent cytotoxic T cell responses to soluble proteins (2000)

Receptors for conserved molecular patterns associated with microbial pathogens induce synthesis of co-stimulatory molecules and cytokines in immature dendritic cells (DC), as do antigen-reactive CD4 T helper cells via CD40 signaling. Once activated, antigen-presenting DC may activate CD8 T cell responses in a T helper cell-independent fashion. Using immunostimulatory CpG-oligonucleotides (ODN) mimicking bacterial CpG-DNA, we tested whether CpG-DNA bypasses the need for T helper cells in CTL responses towards proteins by directly activating antigen-presenting DC to transit into professional APC. We describe that immature DC in situ constitutively process soluble proteins and generate CD8 T cell determinants yet CD8 T cell responses remain abortive. Induction of primary antigen-specific CD8 cytotoxic T lymphocyte (CTL)-mediated responses becomes initiated in wild-type as well as T helper cell-deficient mice, provided soluble protein and CpG-ODN are draining into the same lymph node. Specifically we show that CpG-ODN trigger antigen-presenting immature DC within the draining lymph node to acutely up-regulate co-stimulatory molecules and produce IL-12. These results provide new insights for generating in vivo efficient CTL responses to soluble proteins which may influence vaccination strategies.
A new approach to chemotherapy based on molecular biology and nucleic acid chemistry: Matagen (masking tape for gene expression). (1987)

The nucleotide sequences of genes contain information which can potentially be used to understand gene function and thus the biological properties of living organisms. This information can also be used to develop innovative new strategies for chemotherapy employing sequence-specific non-ionic oligonucleoside methylphosphonates. These oligonucleotide analogs, termed Matagen (an acronym for masking tape for gene expression), have the following properties: (1) the negatively charged phosphodiester linkage normally found in nucleic acids is replaced with a non-charged methylphosphonate group which confers increased lipophilicity to the oligomer; (2) the oligomers form stable hydrogen-bonded complexes with complementary nucleic acid sequences and retain the fidelity of Watson-Crick base pairing; (3) the lipophilic oligomers cross the cell membrane and also enter various organs of the body; and (4) the methylphosphonate backbone is inherently resistant to nuclease hydrolysis and thus oligomers are taken up intact from cell culture media and remain stable within the cellular environment. Two general strategies are used to block gene expression by Matagens at the mRNA level in mammalian cells. In the first approach, Matagens complementary to specific sites such as the initiation codon region are used to block translation of mRNA. Thus Matagens specifically inhibit translation of rabbit globin mRNA in cell-free systems and rabbit reticulocytes, and vesicular stomatitis virus protein synthesis, but not cellular protein synthesis, in virus-infected cells. In the second approach, Matagens complementary to splice junctions of precursor mRNAs are used to inhibit splicing. For example, a Matagen complementary to the donor splice junction of simian virus 40 (SV40) large T-antigen mRNA inhibits T-antigen synthesis in SV40-infected cells, and a Matagen complementary to the acceptor splice junction of herpes simplex virus (HSV) immediate early pre-mRNA 4 + 5 inhibits HSV replication in virus-infected cells. Two new types of Matagen, one derivatized with the photoactivatable cross-linking group psoralen and the other derivatized with a hydroxyl radical-producing group, EDTA-Fe(II), have been designed to improve the efficacy of Matagen and to overcome some of the problems inherent in physical binding of Matagens to complementary nucleic acids. The Matagen approach provides a new way to design antiviral and chemotherapeutic agents in a rational manner. It combines nucleic acid chemistry and chemotherapy to form a common basis for drug development as well as to provide fundamental knowledge about organisms and humans.
Enhanced Electron Transfer Dissociation of Peptides Modified at C-terminus with Fixed Charges (2012)

The impact of the conversion of carboxylates in peptides to basic or fixed charge sites on the outcome of electron transfer dissociation (ETD) is evaluated with respect to ETD efficiency and the number of diagnostic sequence ions. Four reagents, including benzylamine (BA), 1-benzylpiperazine (BZP), carboxymethyl trimethylammonium chloride hydrazide (GT), and (2-aminoethyl)trimethylammonium chloride hydrochloride (AETMA), were used for the carboxylate derivatization, with the first two replacing the acidic carboxylate groups with basic functionalities and the latter two introducing fixed charge sites. The ETD efficiencies and Xcorr scores were compared for both nonderivatized and derivatized tryptic and Glu-C peptides from cytochrome c. Derivatization of the carboxylate increases the average charge states, the number of fragment ions, and the dissociation efficiencies of peptides, especially for the fixed charge reagent, AETMA.
Melanoma cell lines from different stages of progression and their biological and molecular analyses (1997)

The biological and molecular characteristics of cell lines from metastatic melanomas have been extensively studied but less is known about cells from the biologically earliest stage of primary melanoma. The overall success rate of establishing permanent cell lines from such lesions is only 10% of that for biologically late primary or metastatic melanomas, although our laboratory now has eight cell lines available. The cells are immortal but show reduced or no proliferation in soft agar and immunodeficient mice when compared with primary melanomas from the biologically advanced vertical growth phase. Metastatic melanoma cell lines from patients with familial melanoma or xeroderma pigmentosum are biologically similar to those from patients with spontaneous melanomas. Irrespective of the malignant stages, deletions and mutations can occur in exons 1-3 of the p16INK4A gene. DNA fingerprinting was then employed to demonstrate the uniqueness of individual cell lines and to confirm the identity of cell lines derived from same patients. These cell lines are an excellent resource to investigate melanoma progression.
The relationship between class I binding affinity and immunogenicity of potential cytotoxic T cell epitopes. (1994)

The relationship between binding affinity for HLA class I molecules and immunogenicity of discrete peptide epitopes has been analyzed in two different experimental approaches. In the first approach, the immunogenicity of potential epitopes ranging in MHC binding affinity over a 10,000-fold range was analyzed in HLA-A*0201 transgenic mice. In the second approach, the antigenicity of approximately 100 different hepatitis B virus (HBV)-derived potential epitopes, all carrying A*0201 binding motifs, was assessed by using PBL of acute hepatitis patients. In both cases, it was found that an affinity threshold of approximately 500 nM (preferably 50 nM or less) apparently determines the capacity of a peptide epitope to elicit a CTL response. These data correlate well with class I binding affinity measurements of either naturally processed peptides or previously described T cell epitopes. Taken together, these data have important implications for the selection of epitopes for peptide-based vaccines, and also formally demonstrate the crucial role of determinant selection in the shaping of T cell responses. Because in most (but not all) cases, high affinity peptides seem to be immunogenic, our data also suggest that holes in the functional T cell repertoire, if they exist, may be relatively rare.
Laboratory Evaluations of Insecticide Product Efficacy for Control of Cimex lectularius (2006)

Journal Article Laboratory Evaluations of Insecticide Product Efficacy for Control of Cimex lectularius Get access David J. Moore, David J. Moore Department of Entomology, Virginia Tech, Blacksburg, VA 24061 Search for other works by this author on: Oxford Academic PubMed Google Scholar Dini M. Miller Dini M. Miller 1 Department of Entomology, Virginia Tech, Blacksburg, VA 24061 1Corresponding author, e-mail: dinim@vt.edu. Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Economic Entomology, Volume 99, Issue 6, 1 December 2006, Pages 2080–2086, https://doi.org/10.1093/jee/99.6.2080 Published: 01 December 2006 Article history Received: 01 December 2005 Accepted: 28 June 2006 Published: 01 December 2006
Camera/IMU Boresight Calibration: New Advances and Performance Analysis (2002)

TM software of Applanix Corporation. First, a brief discussion is presented to summarize the software capabilities in a descriptive fashion. Then, a number of analytical tools implemented in the software, which are needed to do quality assurance and quality control of the GPS, IMU, image, ground control, and datum issues, are briefly introduced. An analytical study has been done to test the analytical tools of the software. This study has been done using real sets of data collected by HJW GeoSpatial Inc of Oakland California, and by the pilot centre (University of Hannover) of the European Organization for Experimental Photogrammetric Research (OEEPE). All data sets were acquired by a 9” x 9” film camera equipped by a 6” lens cone and by Applanix POS/AV TM 510 system. In addition, all data sets had a good number, distribution, and accuracy of ground control points, quality image measurements, and good GPS and IMU data. This allowed starting off with good quality data sets where biases and noises were introduced intentionally for analysis purposes. Different imaging configurations have been studied. All data sets have been run with and without ground control points where their effect is analysed. A summary of the results and analysis is presented together with the relevant references that discussed this topic.
Extension of phase correlation to subpixel registration (2002)

In this paper, we have derived analytic expressions for the phase correlation of downsampled images. We have shown that for downsampled images the signal power in the phase correlation is not concentrated in a single peak, but rather in several coherent peaks mostly adjacent to each other. These coherent peaks correspond to the polyphase transform of a filtered unit impulse centered at the point of registration. The analytic results provide a closed-form solution to subpixel translation estimation, and are used for detailed error analysis. Excellent results have been obtained for subpixel translation estimation of images of different nature and across different spectral bands.
DB2 Parallel Edition (1995)

The rate of increase in database size and response-time requirements has outpaced advancements in processor and mass storage technology. One way to satisfy the increasing demand for processing power and input/output bandwidth in database applications is to have a number of processors, loosely or tightly coupled, serving database requests concurrently. Technologies developed during the last decade have made commercial parallel database systems a reality, and these systems have made an inroad into the stronghold of traditionally mainframe-based large database applications. This paper describes the DB2® Parallel Edition product that evolved from a prototype developed at IBM Research in Hawthorne, New York, and now is being jointly developed with the IBM Toronto laboratory.
PIK3CA mutations in breast cancer: A potential predictive marker (2006)

639 Background: Somatic mutations in phosphoinositide-3-kinase catalytic alpha (PIK3CA) activates Akt, attenuates apoptosis and promotes tumor invasion. The common mutations E545K and H1047R confer resistance to paclitaxel in immortalized breast cancer cell lines. We screened primary breast cancers for PIK3CA mutations in the helical and catalytic domains. Methods: Tumor samples from 61 stage I-III breast cancer patients treated with adjuvant chemo- and/or hormone therapy were examined for PIK3CA mutations E542K, E545K and H1047R by the 5-nuclease assay (TaqMan) using the ABI Prism 7900HT Sequence Detection System. Results: Mutations were found in 17 patients (27.9%): 6 E542K; 5 E545K; 6 H1047R. PIK3CA mutations were not associated with expression of estrogen or progesterone receptors, lymph node metastases, or ERBB2 overexpression. (See table ) Conclusions: PIK3CA mutations occur frequently in breast cancer and could be a novel predictive marker of chemoresistance and an appealing target for therapeutic inhibition, independent of hormone receptors and ERBB2 status. [Table: see text] No significant financial relationships to disclose.
Immunogenicity and Protection Against Influenza H7N3 in Mice by Modified Vaccinia Virus Ankara Vectors Expressing Influenza Virus Hemagglutinin or Neuraminidase (2018)

Influenza subtypes such as H7 have pandemic potential since they are able to infect humans with severe consequences, as evidenced by the ongoing H7N9 infections in China that began in 2013. The diversity of H7 viruses calls for a broadly cross-protective vaccine for protection. We describe the construction of recombinant modified vaccinia virus Ankara (MVA) vectors expressing the hemagglutinin (HA) or neuraminidase (NA) from three H7 viruses representing both Eurasian and North American H7 lineages - A/mallard/Netherlands/12/2000 (H7N3), A/Canada/rv444/2004 (H7N3), and A/Shanghai/02/2013 (H7N9). These vectors were evaluated for immunogenicity and protective efficacy against H7N3 virus in a murine model of intranasal challenge. High levels of H7-, N3-, and N9-specific antibodies, including neutralizing antibodies, were induced by the MVA-HA and MVA-NA vectors. Mice vaccinated with MVA vectors expressing any of the H7 antigens were protected, suggesting cross-protection among H7 viruses. In addition, MVA vectors expressing N3 but not N9 elicited protection against H7N3 virus challenge. Similar outcomes were obtained when immune sera from MVA vector-immunized mice were passively transferred to naïve mice prior to challenge with the H7N3 virus. The results support the further development of an MVA vector platform as a candidate vaccine for influenza strains with pandemic potential.
Functional silencing of hepatic microsomal glucose‐6‐phosphatase gene expression in vivo by adenovirus‐mediated delivery of short hairpin RNA (2004)

An expression cassette containing mouse U6 polymerase III promoter directing expression of short hairpin RNA (shRNA) targeting murine microsomal glucose-6-phosphatase (G6P) transcript was generated. This construct was packaged into an adenoviral (AdV) backbone and viral stocks generated. Mice injected intravenously with AdV-G6PshRNA exhibited a significant reduction in postprandial glucose levels and had significantly elevated steady-state hepatic glycogen stores. Target gene silencing was confirmed by measurements demonstrating a significant reduction in both hepatic G6P transcript level and phosphohydrolase activity. These findings provide evidence that AdV delivery of expressed shRNA can be a productive tool to explore gene function in vivo.