gebhart/patrepeval huggingface dataset:
Role of natriuretic peptide clearance receptor in in vivo control of C-type natriuretic peptide (1995)

C-type natriuretic peptide (CNP) is a newly described 22-amino acid peptide of endothelial cell origin, which has selective cardiovascular actions and is structurally related to atrial natriuretic peptide (ANP). Recent in vitro studies have demonstrated that an important regulatory pathway for the clearance of natriuretic peptides involves binding to a common clearance receptor [natriuretic peptide C receptor (NPR-C)]. Although CNP has also been identified as a ligand for NPR-C in binding assays, no studies have defined the in vivo interaction of CNP with NPR-C. CNP (10 ng.kg-1.min-1) followed by C-ANP-(4-23), a specific ligand for NPR-C blockade, was infused intravenously in two groups (both n = 7) of anesthetized dogs at two different doses (0.1 or 1.0 micrograms.kg-1.min-1) to permit calculation of total metabolic clearance rate (TMCR). C-ANP-(4-23) increased circulating CNP and reduced TMCR in both groups. Pulmonary metabolic clearance rate was negative at baseline, suggesting a net secretion of CNP across the lung, which was increased during CNP infusion and was abolished with NPR-C blockade. Renal and femoral metabolic clearance rates were positive at baseline and increased with CNP infusion. A decrease in cardiac output and cardiac filling pressures in response to CNP administration was potentiated by NPR-C blockade. We conclude that 1) circulating CNP achieved by CNP infusion is regulated by NPR-C in vivo, 2) the pulmonary circulation is a possible site of CNP secretion, 3) the renal and peripheral circulations are sites of CNP clearance, and 4) NPR-C blockade potentiates the selective cardiovascular actions of CNP.
Medical Instrumentation-Application and Design (1978)

Basic Concepts of Medical Instrumentation Basic Sensors and Principles Amplifiers and Signal Processing The Origin of Biopotentials Biopotential Electrodes Biopotential Amplifiers Blood Pressure and Sound Measurement of Flow and Volume of Blood Measurements of the Respiratory System Chemical Biosensors Clinical Laboratory Instrumentation Medical Imaging Systems Therapeutic and Prosthetic Devices Electrical Safety.
Tripping the switch fantastic: how a protein kinase cascade can convert graded inputs into switch-like outputs (1996)

Recent experimental work has shown that the mitogen-activated protein (MAP) kinase cascade can convert graded inputs into switch-like outputs. The cascade could therefore filter out noise (signals of insufficient magnitude or duration) and still respond decisively to supra-threshold stimuli. Here, we explore the biochemical mechanisms likely to be at the root of this behavior.
Application of the sinc basis moment method to the reconstruction of infinite circular cylinders (1988)

A solution of the ultrasonic scattering and inverse scattering problem has been obtained by solving the inhomogeneous Helmholtz wave equation by the sinc basis moment method. In this numerical study, the algorithm of S.A. Johnson and M.L. Tracy (1983) has been applied to the reconstruction of an infinite circular cylinder that is subject to an incident cylindrical wave of ultrasound and is surrounded by a homogeneous coupling medium. For weak scattering cylinders, successful reconstructions have been obtained using the known exact solution for the scattered field as the input data for the algorithm. A detailed discussion of sampling requirements for this algorithm is presented, and the threshold derived correlates well with results of a numerical study of variation of the sampling density. Effects of varying object contrast, object size, grid size, sampling density, and method of iteration are investigated. Because the algorithm is slow, optimization of computation is described.
Biological Activity of Soluble CD100. II. Soluble CD100, Similarly to H-SemaIII, Inhibits Immune Cell Migration (2001)

CD100 is a human 150-kDa homodimer expressed at the surface of most hemopoietic cells, and its gene belongs to the Ig and semaphorin gene families. Semaphorin genes encode soluble and membrane-bound proteins, most of which have been shown to act as chemorepellents on growth cone guidance. CD100 is discrete, as it is a transmembrane leukocyte surface molecule that can also exist in a soluble form. While our previous studies using mAbs suggested that the transmembrane form of CD100 plays a role in lymphocyte activation, no function was shown for its soluble form. Here, we investigated the effect of soluble CD100 in a cell migration assay; both CD100 spontaneously shed from a stable transfectant and soluble recombinant CD100 inhibited spontaneous and chemokine-induced migration of human monocytes. Interestingly, only the dimeric form of CD100 exerted an effect. Moreover, soluble CD100 inhibited migration of cells from monocytic and B cell lineages. A similar inhibitory effect on migration was observed with H-SemaIII, but not H-SemaIV, semaphorins. In addition, both CD100 and H-SemaIII were recognized by two CD100 mAbs in an ELISA, and one of these mAb abolished the inhibitory effect of each of these semaphorins. We also provide evidence that CD100 and H-SemaIII act through the same receptor on immune cells, which is not neuropilin-1. Furthermore, we describe a function on immune cells for H-SemaIII, a semaphorin to date only studied in the nervous system.
TV-Anytime based personalized bi-directional metadata service system (2006)

Nowadays, as the broadcast media are increased and the convergence of broadcast and communications is advanced, users become consumed abundant contents by various devices, at anytime, in anywhere. Moreover, the desire for searching and consuming user-preferred contents is increased. For reasons mentioned above, TV-Anytime metadata standard has been defined, which is the one for supporting an anytime TV program service. In this paper, TV-Anytime standard based service system is proposed, which provides two personalized content services reflecting the user's preference and terminal environment. One is a media library service and the other is a target advertisement service. Media library service is one for transmitting contents selected by user from consumable contents list provided by bi-directional metadata server. For content transmission, two methods - file download and streaming are supported and for a personalized service, it is provided that an adapted streaming service based on multimedia modality conversion technique according to a terminal type such as Set-Top Box and PDA and a usage environment such as network condition. Target advertisement service is one of main broadcast services for end-users, broadcasting stations and advertisers. In bidirectional metadata server, a user profile such as gender and age is reasoned from TV usage history based on TV-Anytime standard and Multistage Classifier algorithm. By this result, targeted advertisement contents are transmitted to end-user.
Identification of loci determining susceptibility to the lethal effects of amyloid precursor protein transgene overexpression (2004)

Phenotypes produced by expression of human amyloid precursor protein (APP) transgenes vary depending on the genetic background of the mouse. FVB/N mice overexpressing human APP695 develop a central nervous system disorder and die prematurely, precluding development of Abeta peptide amyloid plaques. 129S6 mice are resistant to the lethal effects of APP overexpression, allowing sufficient levels of Abeta expression for the development of amyloid plaques and age-dependent memory deficits. To identify the genes that determine susceptibility or resistance to APP we analyzed crosses involving FVB/NCr and 129S6.Tg2576 mice that overexpress 'Swedish' mutant (K670N, M671L) APP695. APP transgene-positive FVB129S6F1 (F1) mice are resistant to the lethal effects of APP overexpression, so FVBxF1 backcross and F2 intercross offspring were produced. Analysis of age of death as a quantitative trait revealed significant linkage to loci on proximal chromosome 14 and on chromosome 9; 129S6 alleles protect against the lethal effects of APP. Within the chromosome 14 interval are segments homologous to regions on human chromosome 10 that have been linked to late onset Alzheimer's disease or to levels of Abeta peptide in plasma. However, analysis of plasma Abeta peptide concentrations at 6 weeks in backcross offspring produced no significant linkage. Similarly, elevation of human Abeta peptide concentrations by expression of mutant presenilin transgenes did not increase the proportion of mice dying prematurely, suggesting that early death reflects effects of APP or fragments other than Abeta.
Activation of the Alternative Complement Pathway in Vitreous is Controlled by Genetics in Age-Related Macular Degeneration (2012)

Increased activation of the alternative complement pathway in vitreous was controlled by disease stage and genetic variation in the complement pathway, supporting a role for complement activation in AMD disease pathogenesis.
On Self Modulation for Generative Adversarial Networks (2018)

Training Generative Adversarial Networks (GANs) is notoriously challenging. We propose and study an architectural modification, self-modulation, which improves GAN performance across different data sets, architectures, losses, regularizers, and hyperparameter settings. Intuitively, self-modulation allows the intermediate feature maps of a generator to change as a function of the input noise vector. While reminiscent of other conditioning techniques, it requires no labeled data. In a large-scale empirical study we observe a relative decrease of $5\%-35\%$ in FID. Furthermore, all else being equal, adding this modification to the generator leads to improved performance in $124/144$ ($86\%$) of the studied settings. Self-modulation is a simple architectural change that requires no additional parameter tuning, which suggests that it can be applied readily to any GAN.
Fluid Orientation on a Tabletop Display: Integrating Rotation and Translation (2004)

Previous research has shown that rotation and orientation of items plays three major roles during the course of tabletop collaboration: comprehension, coordination and communication. Based on these roles of orientation, we designed a novel tabletop rotation mechanism, Rotate N Translate (RNT), which provides integral control of rotation and translation using only a single touch-point for input. We present an empirical evaluation comparing RNT to the standard rotation mechanism that separated control of rotation and translation. Results of this study indicate RNT is more efficient than the separate mechanism and better supports the communicative and comprehensive roles of orientation, without sacrificing support for coordination.
Whole-body and intravital optical imaging of angiogenesis in orthotopically implanted tumors (2001)

The development of drugs for the control of tumor angiogenesis requires a simple, accurate, and economical assay for tumor-induced vascularization. We have adapted the orthotopic implantation model to angiogenesis measurement by using human tumors labeled with Aequorea victoria green fluorescent protein for grafting into nude mice. The nonluminous induced capillaries are clearly visible against the very bright tumor fluorescence examined either intravitally or by whole-body luminance in real time. The orthotopic implantation model of human cancer has been well characterized, and fluorescence shadowing replaces the laborious histological techniques for determining blood vessel density. Intravital images of orthotopically implanted human pancreatic tumors clearly show angiogenic capillaries at both primary and metastatic sites. A quantitative time course of angiogenesis was determined for an orthotopically growing human prostate tumor periodically imaged intravitally in a single nude mouse over a 19-day period. Whole-body optical imaging of tumor angiogenesis was demonstrated by injecting fluorescent Lewis lung carcinoma cells into the s.c. site of the footpad of nude mice. The footpad is relatively transparent, with comparatively few resident blood vessels, allowing quantitative imaging of tumor angiogenesis in the intact animal. Capillary density increased linearly over a 10-day period as determined by whole-body imaging. Similarly, the green fluorescent protein-expressing human breast tumor MDA-MB-435 was orthotopically transplanted to the mouse fat pad, where whole-body optical imaging showed that blood vessel density increased linearly over a 20-week period. These powerful and clinically relevant angiogenesis mouse models can be used for real-time in vivo evaluation of agents inhibiting or promoting tumor angiogenesis in physiological microenvironments.
Influence of Size, Surface, Cell Line, and Kinetic Properties on the Specific Binding of A33 Antigen-Targeted Multilayered Particles and Capsules to Colorectal Cancer Cells (2007)

There has been increased interest in the use of polymer capsules formed by the layer-by-layer (LbL) technique as therapeutic carriers to cancer cells due to their versatility and ease of surface modification. We have investigated the influence of size, surface properties, cell line, and kinetic parameters such as dosage (particle concentration) and incubation time on the specific binding of humanized A33 monoclonal antibody (huA33 mAb)-coated LbL particles and capsules to colorectal cancer cells. HuA33 mAb binds to the A33 antigen present on almost all colorectal cancer cells and has demonstrated great promise in clinical trials as an immunotherapeutic agent for cancer therapy. Flow cytometry experiments showed the cell binding specificity of huA33 mAb-coated particles to be size-dependent, with the optimal size for enhanced selectivity at approximately 500 nm. The specific binding was improved by increasing the dosage of particles incubated with the cells. The level of specific versus nonspecific binding was compared for particles terminated with various polyelectrolytes to examine the surface dependency of antibody attachment and subsequent cell binding ability. The specific binding of huA33 mAb-coated particles is also reported for two colorectal cancer cell lines, with an enhanced binding ratio between 4 and 10 obtained for the huA33 mAb-functionalized particles. This investigation aims to improve the level of specific targeting of LbL particles, which is important in targeted drug and gene delivery applications.
A new therapy for portal systemic encephalopathy. (1986)

This report describes a new therapeutic approach to the treatment of adults with portal systemic encephalopathy using sodium benzoate and sodium phenylacetate, two compounds that increase conjugated nitrogen excretion in the urine and that have been shown to be an effective treatment for children with congenital hyperammonemia. The study was a double-blind cross-over study in which each patient was his own control. All patients either improved their mental status (six of eight) or maintained a mental status comparable to that achieved with conventional therapy [lactulose or neomycin (two of eight)]. All decreased their blood ammonias on these medications and seven of eight improved their portal systemic encephalopathy index. It is suggested that these medications may be useful in the treatment of portal systemic encephalopathy.
A Multiplicative Weights Mechanism for Privacy-Preserving Data Analysis (2010)

We consider statistical data analysis in the interactive setting. In this setting a trusted curator maintains a database of sensitive information about individual participants, and releases privacy-preserving answers to queries as they arrive. Our primary contribution is a new differentially private multiplicative weights mechanism for answering a large number of interactive counting (or linear) queries that arrive online and may be adaptively chosen. This is the first mechanism with worst-case accuracy guarantees that can answer large numbers of interactive queries and is efficient (in terms of the runtime's dependence on the data universe size). The error is asymptotically optimal in its dependence on the number of participants, and depends only logarithmically on the number of queries being answered. The running time is nearly linear in the size of the data universe. As a further contribution, when we relax the utility requirement and require accuracy only for databases drawn from a rich class of databases, we obtain exponential improvements in running time. Even in this relaxed setting we continue to guarantee privacy for any input database. Only the utility requirement is relaxed. Specifically, we show that when the input database is drawn from a smooth distribution - a distribution that does not place too much weight on any single data item - accuracy remains as above, and the running time becomes poly-logarithmic in the data universe size. The main technical contributions are the application of multiplicative weights techniques to the differential privacy setting, a new privacy analysis for the interactive setting, and a technique for reducing data dimensionality for databases drawn from smooth distributions.
Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from POU1F1- and NR5A1-Cell Lineages (2020)

Spliceosome components are significant constituents of the PA molecular machinery and could be used as molecular markers and therapeutic targets. Splicing-related genes and mRNA-isoforms profiles characterize tumor subtypes.
Virus-associated hemophagocytic syndromeA benign histiocytic proliferation distinct from malignant histiocytosis (1979)

Nineteen patients whose bone marrow smears showed histiocytic hyperplasia with prominent hemophagocytosis were found to have a clinicopathologic syndrome associated with active viral infection. High fever, constitutional symptoms, liver function, and coagulation abnormalities and peripheral blood cytopenias were characteristic findings. Hepatosplenomegaly, lymphadenopathy, bilateral pulmonary infiltrates, and skin rash were often present. Fourteen of the patients were immunosuppressed. Active infection by herpes group viruses was documented in 14 patients and by adenovirus in 1. The bone marrow of most patients also showed decreased granulopoiesis and erythropoiesis with normal to increased numbers of megakaryocytes. Treatment generally consisted of supportive therapy and withdrawal of immunosuppressive drugs. Thirteen patients recovered. Lymph node biopsy and autopsy material showed generalized histiocytic hyperplasia with hemophagocytosis. The relationship of this disorder to familial hemophagocytic reticulosis, familial erythrophagocytic lymphohistiocytosis, histiocytic medullary reticulosis, and malignant histiocytosis is discussed. Immunosuppressive and cytotoxic therapy may be contraindicated in the treatment of this virus-associated syndrome.
Antinociception by Epidural and Systemic α2-Adrenoceptor Agonists and Their Binding Affinity in Rat Spinal Cord and Brain (2000)

Spinal antinociception caused by the epidural administration of alpha(2) agonists is well correlated with their binding affinity to spinal alpha(2)-adrenoceptors.
Nucleolar ultrastructure in bovine nuclear transfer embryos (1999)

Nuclear transfer experiments in mammals have attempted to reprogram a donor nucleus to a state equivalent to the zygotic one. Reprogramming of the donor nucleus is, among other features, indicated by a synthesis of ribosomal RNA (rRNA). The initiation of rRNA synthesis is simultaneously reflected in nuclear morphology as a transformation of the nucleolus precursor body into a functional rRNA synthesising nucleolus with a characteristic ultrastructure. We examined nucleolar ultrastructure in bovine in vitro produced (control) embryos and in nuclear transfer embryos reconstructed from a MII phase (nonactivated) or S phase (activated) cytoplasts. Control embryos were fixed at the two-, four-, early eight- and late eight-cell stages; nuclear transfer embryos were fixed at 1 and 3 hr post fusion and at the two-, four-, and eight-cell stages. Control embryos possessed a nucleolar precursor body throughout all three cell cycles. In the eight-cell stage embryo, a primary vacuole appeared as an electron lucid area originating in the centre of the nucleolar precursor body. In nuclear transfer embryos reconstructed from nonactivated cytoplasts, the nuclear envelope was fragmented or completely broken down at 1 hr after fusion and, by 3 hr after fusion, it was restored again. At this time, the reticulated fibrillo-granular nucleolus had an almost round shape. The nucleolar precursor body seen in the two-cell stage nuclear transfer embryos consisted of intermingled filamentous components and secondary vacuoles. A nucleolar precursor body typical for the two-cell stage control embryos was never observed. None of the reconstructed embryos of this group reached the eight-cell stage. Nuclear transfer embryos reconstructed from activated cytoplasts, in contrast, exhibited a complete nuclear envelope at all time intervals after fusion. In the two-cell stage nuclear transfer embryo, the originally reticulated nucleolus of the donor blastomere had changed into a typical nucleolar precursor body consisting of a homogeneous fibrillar structure. A primary vacuole appeared in the four-cell stage nuclear transfer embryos, which was one cell cycle earlier than in control embryos. Only nuclear transfer embryos reconstructed from activated cytoplasts underwent complete remodelling of the nucleolus. The reorganisation of the donor nucleolar architecture into a functionally active nucleolus was observed as early as in the four-cell stage nuclear transfer embryo. These ultrastructural observations were correlated with our autoradiographic data on the initiation of RNA synthesis in nuclear transfer embryos. Mol. Reprod. Dev. 52:253–263, 1999. © 1999 Wiley-Liss, Inc.
The Transcriptome Profile of Human Embryonic Stem Cells as Defined by SAGE (2004)

Human embryonic stem (ES) cell lines that have the ability to self-renew and differentiate into specific cell types have been established. The molecular mechanisms for self-renewal and differentiation, however, are poorly understood. We determined the transcriptome profiles for two proprietary human ES cell lines (HES3 and HES4, ES Cell International), and compared them with murine ES cells and other human tissues. Human and mouse ES cells appear to share a number of expressed gene products although there are numerous notable differences, including an inactive leukemia inhibitory factor pathway and the high preponderance of several important genes like POU5F1 and SOX2 in human ES cells. We have established a list of genes comprised of known ES-specific genes and new candidates that can serve as markers for human ES cells and may also contribute to the "stemness" phenotype. Of particular interest was the downregulation of DNMT3B and LIN28 mRNAs during ES cell differentiation. The overlapping similarities and differences in gene expression profiles of human and mouse ES cells provide a foundation for a detailed and concerted dissection of the molecular and cellular mechanisms governing their pluripotency, directed differentiation into specific cell types, and extended ability for self-renewal.
Parametrically Activated Entangling Gates Using Transmon Qubits (2018)

A central challenge in building a scalable quantum computer with superconducting qubits is the execution of high-fidelity two-qubit gates in the presence of many resonant elements. As more elements are added to the architecture, and as the multiplicity of their couplings grows, the design's frequency space becomes crowded, and performance suffers. The authors present a way to address this difficulty: selective activation of interactions between transmon qubits of fixed frequency and those of tunable frequency. This activation depends on both the amplitude and frequency of modulation, and using the amplitude as an additional condition for resonance alleviates frequency crowding.