gebhart/patrepeval huggingface dataset:
The 5f3 vs. 4f3. Routes to and properties of highly reactive neodymium(III) hydrocarbyl and hydride complexes (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe 5f3 vs. 4f3. Routes to and properties of highly reactive neodymium(III) hydrocarbyl and hydride complexesHeiko Mauermann, Paul N. Swepston, and Tobin J. MarksCite this: Organometallics 1985, 4, 1, 200–202Publication Date (Print):January 1, 1985Publication History Published online1 May 2002Published inissue 1 January 1985https://pubs.acs.org/doi/10.1021/om00120a036https://doi.org/10.1021/om00120a036research-articleACS PublicationsRequest reuse permissionsArticle Views218Altmetric-Citations84LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Assessment of serum N-terminal pro-B-type natriuretic peptide concentration for differentiation of congestive heart failure from primary respiratory tract disease as the cause of respiratory signs in dogs (2009)

Measuring serum NT-proBNP concentration in dogs with respiratory signs helps to differentiate between congestive heart failure and primary respiratory tract disease as an underlying cause.
Julian Apostata. Edited by Klein Richard. Wege der Forschung, Volume 509. Darmstadt, West Germany: Wissenschaftliche Buchgesellschaft, 1978. vi + 531. (1980)

An abstract is not available for this content so a preview has been provided. Please use the Get access link above for information on how to access this content.
Detection of pathogens from periodontal lesions (2004)

PCR is an effective tool for detecting periodontal pathogens in subgingival samples, providing a faster and safer diagnostic tool of periodontal diseases. The method's sensitivity and specificity is conditioned by the choice of the set of primers used.
Epitaxial SnO2 thin films grown on (1012) sapphire by femtosecond pulsed laser deposition (2002)

An ultrafast (100 fs) Ti sapphire laser (780 nm) was used for the deposition of SnO2 thin films. The laser-induced plasma generated from the SnO2 target was characterized by optical emission spectroscopy and electrostatic energy analysis. It was found that the ionic versus excited-neutral component ratio in the plasma plume depends strongly on the amount of background oxygen introduced to the deposition chamber. Epitaxial SnO2 films with high quality and a very smooth surface were deposited on the (1̄012) sapphire substrate fabricated at 700 °C with an oxygen background pressure of ∼0.1 mTorr. The films are single crystalline with the rutile structure, resulting from the high similarity in oxygen octahedral configurations between the sapphire (1̄012) surface and the SnO2 (101) surface. Hall effect measurements showed that the electron mobility of the SnO2 film is lower than that of bulk single crystal SnO2, which is caused by the scattering of conduction electrons at the film surface, substrate/film interface, and crystal defects.
Direct Hemin Transfer from IsdA to IsdC in the Iron-regulated Surface Determinant (Isd) Heme Acquisition System of Staphylococcus aureus (2008)

The iron-regulated surface determinants (Isd) of Staphylococcus aureus, including surface proteins IsdA, IsdB, IsdC, and IsdH and ATP-binding cassette transporter IsdDEF, constitute the machinery for acquiring heme as a preferred iron source. Here we report hemin transfer from hemin-containing IsdA (holo-IsdA) to hemin-free IsdC (apo-IsdC). The reaction has an equilibrium constant of 10 +/- 5 at 22 degrees C in favor of holo-IsdC formation. During the reaction, holo-IsdA binds to apo-IsdC and then transfers the cofactor to apo-IsdC with a rate constant of 54.3 +/- 1.8 s(-1) at 25 degrees C. The transfer rate is >70,000 times greater than the rate of simple hemin dissociation from holo-IsdA into solvent (k transfer = 54.3 s(-1) versus k -hemin = 0.00076 s(-1)). The standard free energy change, Delta G 0, is -27 kJ/mol for the formation of the holo-IsdA-apo-IsdC complex. IsdC has a higher affinity for hemin than IsdA. These results indicate that the IsdA-to-IsdC hemin transfer is through the activated holo-IsdA-apo-IsdC complex and is driven by the higher affinity of apo-IsdC for the cofactor. These findings demonstrate for the first time in the Isd system that heme transfer is rapid, direct, and affinity-driven from IsdA to IsdC. These results also provide the first example of heme transfer from one surface protein to another surface protein in Gram-positive bacteria and, perhaps most importantly, indicate that the mechanism of activated heme transfer, which we previously demonstrated between the streptococcal proteins Shp and HtsA, may apply in general to all bacterial heme transport systems.
Artificial Vision for the Blind by Electrical Stimulation of the Visual Cortex (1979)

Artificial vision for the blind may be feasible by interfacing a television camera with electronics stimulating the visual cortex. The status of a major collaborative effort involving the College of Physicians and Surgeons of Columbia University, the University of Utah, and the University of Western Ontario is reviewed. Results have been very encouraging, although much work remains to be done.
Definition of Mycobacterium tuberculosis culture filtrate proteins by two-dimensional polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and electrospray mass spectrometry (1997)

A number of the culture filtrate proteins secreted by Mycobacterium tuberculosis are known to contribute to the immunology of tuberculosis and to possess enzymatic activities associated with pathogenicity. However, a complete analysis of the protein composition of this fraction has been lacking. By using two-dimensional polyacrylamide gel electrophoresis, detailed maps of the culture filtrate proteins of M. tuberculosis H37Rv were generated. In total, 205 protein spots were observed. The coupling of this electrophoretic technique with Western blot analysis allowed the identification and mapping of 32 proteins. Further molecular characterization of abundant proteins within this fraction was achieved by N-terminal amino acid sequencing and liquid chromatography-mass spectrometry. Eighteen proteins were subjected to N-group analysis; of these, only 10 could be sequenced by Edman degradation. Among the most interesting were a novel 52-kDa protein demonstrating significant homology to an alpha-hydroxysteroid dehydrogenase of Eubacterium sp. strain VPI 12708, a 25-kDa protein corresponding to open reading frame 28 of the M. tuberculosis cosmid MTCY1A11, and a 31-kDa protein exhibiting an amino acid sequence identical to that of antigen 85A and 85B. This latter product migrated with an isoelectric point between those of antigen 85A and 85C but did not react with the antibody specific for this complex, suggesting that there is a fourth member of the antigen 85 complex. Novel N-terminal amino acid sequences were obtained for three additional culture filtrate proteins; however, these did not yield significant homology to known protein sequences. A protein cluster of 85 to 88 kDa, recognized by the monoclonal antibodies IT-57 and IT-42 and known to react with sera from a large proportion of tuberculosis patients, was refractory to N-group analysis. Nevertheless, mass spectrometry of peptides obtained from one member of this complex identified it as the M. tuberculosis KatG catalase/peroxidase. Thus, the detailed mapping of M. tuberculosis proteins, combined with state-of-the-art analytical techniques such as mass spectrometry, provides a basis for further analysis and rapid identification of biologically relevant molecules.
Human Biotransformation (2010)

This chapter contains sections titled: Introduction Biotransformation Versus Transport Pharmacokinetic Variation and Therapeutic Index Parent Drug Versus Metabolite Drugs as Victims and Perpetrators Perpetrator–Perpetrator–Victim Interactions Drug Metabolism from a Chemical Perspective Drug Metabolism from an Enzyme Perspective Reactive Metabolites References
Copper chloride, an inhibitor of protein import into chloroplasts (1995)

We have used the oxidant CuCl2 to study its effect on precursor protein import into chloroplasts and on the components involved. CuCl2 reversibly oxidizes thiol groups, which in turn, can form disulfide bridges. Concentrations of 40 microM CuCl2 almost completely inhibit precursor protein binding and subsequent translocation into chloroplasts. This inhibitory effect is reversible by a dithiothreitol treatment. Disulfide bridges, which form upon oxidation by CuCl2, are build up intramolecular and intermolecular, if the thiol groups are in close vicinity to each other. CuCl2 can thus be used as a thiol cleavable crosslinker without an additional spacer distance between the two targets. When purified outer envelope membranes were treated with CuCl2, a hetero oligomeric complex is detected, consisting of OEP86, OEP75 and OEP34, indicating the close vicinity and protein-protein interaction between polypeptides in situ, which are involved in protein translocation into chloroplasts.
Streptavidin production by Streptomyces avidinii in a laboratory fermenter (1992)

Conference Article| February 01 1992 Streptavidin production by Streptomyces avidinii in a laboratory fermenter LINDA A. HENDY; LINDA A. HENDY 1Department of Biological Sciences, Dublin Institute of Technology, Kevin Street, Dublin 8 Search for other works by this author on: This Site PubMed Google Scholar SHARON McGONIGLE; SHARON McGONIGLE 1Department of Biological Sciences, Dublin Institute of Technology, Kevin Street, Dublin 8 Search for other works by this author on: This Site PubMed Google Scholar URSULA MacEVILLY; URSULA MacEVILLY 1Department of Biological Sciences, Dublin Institute of Technology, Kevin Street, Dublin 8 Search for other works by this author on: This Site PubMed Google Scholar RAYMOND RYAN; RAYMOND RYAN 1Department of Biological Sciences, Dublin Institute of Technology, Kevin Street, Dublin 8 Search for other works by this author on: This Site PubMed Google Scholar MICHEAL G. HARRINGTON MICHEAL G. HARRINGTON *Department of Biochemistry, University College Dublin, Dublin 4 Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1992) 20 (1): 72S. https://doi.org/10.1042/bst020072s Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation LINDA A. HENDY, SHARON McGONIGLE, URSULA MacEVILLY, RAYMOND RYAN, MICHEAL G. HARRINGTON; Streptavidin production by Streptomyces avidinii in a laboratory fermenter. Biochem Soc Trans 1 February 1992; 20 (1): 72S. doi: https://doi.org/10.1042/bst020072s Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1992 Biochemical Society1992 Article PDF first page preview Close Modal You do not currently have access to this content.
Crosslinked PMMA as a high-resolution negative resist for electron beam lithography and applications for physics of low-dimensional structures (1996)

We present a novel technique which employs crosslinked PMMA as a high-resolution negative resist for electron beam lithography. The technique allows the patterning of submicrometre features in an insulating layer, thus simplifying the fabrication process of various multilayer devices. We demonstrate this by reference to specific devices and present simple experimental results which prove the usefulness of the technique.
Multispectral imaging in biology and medicine: Slices of life (2006)

Multispectral imaging (MSI) is currently in a period of transition from its role as an exotic technique to its being offered in one form or another by all the major microscopy manufacturers. This is because it provides solutions to some of the major challenges in fluorescence-based imaging, namely ameliorating the consequences of the presence of autofluorescence and the need to easily accommodate relatively high levels of signal multiplexing. MSI, which spectrally characterizes and computationally eliminates autofluorescence, enhances the signal-to-background dramatically, revealing otherwise obscured targets. While this article concentrates on examples derived from liquid-crystal tunable filter-based technology, the intent is to showcase the advantages of multispectral imaging in general. Some technologies used to generate multispectral images are compatible with only particular optical configurations, such as point-scanning laser confocal microscopy. Band-sequential approaches, such as those afforded by liquid-crystal tunable filters (LCTFs), can be conveniently coupled with a variety of imaging modalities, which, in addition to fluorescence microscopy, include brightfield (nonfluorescent) microscopy as well as small-animal, noninvasive in-vivo imaging. Brightfield microscopy is the chosen format for histopathology, which relies on immunohistochemistry to provide molecularly resolved clinical information. However, in contrast to fluorescent labels, multiple chromogens, if they spatially overlap, are much harder to separate and quantitate, unless MSI approaches are used. In-vivo imaging is a rapidly growing field with applications in basic biology, drug discovery, and clinical medicine. The sensitivity of fluorescence-based in-vivo imaging, as with fluorescence microscopy, can be limited by the presence of significant autofluorescence, a limitation which can be overcome through the utilization of MSI.
Multidirectional sorting modes in deterministic lateral displacement devices (2008)

Deterministic lateral displacement (DLD) devices separate micrometer-scale particles in solution based on their size using a laminar microfluidic flow in an array of obstacles. We investigate array geometries with rational row-shift fractions in DLD devices by use of a simple model including both advection and diffusion. Our model predicts multidirectional sorting modes that could be experimentally tested in high-throughput DLD devices containing obstacles that are much smaller than the separation between obstacles.
Protective role of liposome incorporated lipopolysaccharide antigen of Klebsiella pneumoniae in a rat model of lobar pneumonia. (2004)

In a lobar pneumonia model of Klebsiella pneumoniae, the immunoprotective role of free lipoploysaccharide (LPS) and liposome-incorporated LPS was studied. An alteration in the biological activity of the LPS molecule, in terms of its pyrogenicity and lethal toxicity, was observed on incorporation in the liposome. Compared at equal doses, liposome-incorporated LPS was found to be non-pyrogenic and 10 times less toxic than free LPS. Liposome-incorporated LPS was more effective in providing protection against K. pneumoniae induced lobar pneumonia in rats. The immunological mechanism underlying protection revealed involvement of both nonspecific and specific immune response. Alveolar macrophage activation was observed after 4 and 14 days of treatment with the free and liposome-entrapped forms of LPS, respectively. Specific immunity in terms of plaque-forming cells was seen with both forms of LPS. Delayed type hypersensitivity reaction was observed only with liposome-incorporated LPS. It is concluded that a non-toxic and immunogenic form of K. pneumoniae LPS can be obtained by incorporation of the native preparation into liposomes.
TDP-43 facilitates milk lipid secretion by post-transcriptional regulation of Btn1a1 and Xdh (2020)

Milk lipid secretion is a critical process for the delivery of nutrition and energy from parent to offspring. However, the underlying molecular mechanism is less clear. Here we report that TDP-43, a RNA-binding protein, underwent positive selection in the mammalian lineage. Furthermore, TDP-43 gene (Tardbp) loss induces accumulation of large lipid droplets and severe lipid secretion deficiency in mammary epithelial cells to outside alveolar lumens, eventually resulting in lactation failure and pup starvation within three weeks postpartum. In human milk samples from lactating women, the expression levels of TDP-43 is positively correlated with higher milk output. Mechanistically, TDP-43 exerts post-transcriptional regulation of Btn1a1 and Xdh mRNA stability, which are required for the secretion of lipid droplets from epithelial cells to the lumen. Taken together, our results highlights the critical role of TDP-43 in milk lipid secretion, providing a potential strategy for the screening and intervention of clinical lactation insufficiency.
The Family of GFP‐Like Proteins: Structure, Function, Photophysics and Biosensor Applications. Introduction and Perspective (2005)

ABSTRACT In this issue, we offer a symposium‐in‐print that is focused on several new advancements in fundamental research related to the family of GFP (green fluorescent protein)‐like proteins. A few applied aspects are also included to illustrate the impact this amazing set of colored proteins has made on our understanding of cell biology at the molecular level. The six articles presented here cut across several disciplines ranging from biological function to protein structure to photophysical aspects. These highly original pieces of work include both experimental and computational approaches, and will provide the reader with significant insight into current, state‐of‐the‐art research activities in this very dynamic and fast‐paced field. In the first part of this perspective, I will give a brief overview of the history and salient features of GFPs, cite some examples that illustrate their impact on biotechnology, and provide a brief review of the structural and chemical features that lend these proteins their fascinating appearance. In the second part, I will introduce each of the peer‐reviewed contributions of the participating authors.
Identification of the Escherichia coli sohB gene, a multicopy suppressor of the HtrA (DegP) null phenotype (1991)

We cloned and sequenced the sohB gene of Escherichia coli. The temperature-sensitive phenotype of bacteria that carry a Tn10 insertion in the htrA (degP) gene is relieved when the sohB gene is present in the cell on a multicopy plasmid (30 to 50 copies per cell). The htrA gene encodes a periplasmic protease required for bacterial viability only at high temperature, i.e., above 39 degrees C. The sohB gene maps to 28 min on the E. coli chromosome, precisely between the topA and btuR genes. The gene encodes a 39,000-Mr precursor protein which is processed to a 37,000-Mr mature form. Sequencing of a DNA fragment containing the gene revealed an open reading frame which could encode a protein of Mr 39,474 with a predicted signal sequence cleavage site between amino acids 22 and 23. Cleavage at this site would reduce the size of the processed protein to 37,474 Mr. The predicted protein encoded by the open reading frame has homology with the inner membrane enzyme protease IV of E. coli, which digests cleaved signal peptides. Therefore, it is possible that the sohB gene encodes a previously undiscovered periplasmic protease in E. coli that, when overexpressed, can partially compensate for the missing HtrA protein function.
Solved upscaling problems for implementing deammonification of rejection water (2006)

So far, extremely efficient metabolic pathways for nitrogen removal exclusively by autotrophic organisms are well established in scientific literature but not in practice. This paper presents results from the successful implementation of rejection water deammonification in a full-scale single sludge system at the WWTP Strass, Austria. Anaerobic ammonia oxidising biomass has been accumulated during a 2.5 year start-up period when the reactor size was gradually scaled up in the steps. The pH-controlled deammonification system (DEMON) has reached a design capacity of eliminating approximately 300 kg of nitrogen per day. Energy savings outperform expectations, decreasing the mean specific demand for compressed air from 109 m3(kg N)(-1) to 29 m3(kg N)(-1). Dominance of autotrophic metabolism is confirmed by organic effluent loads topping influent loads.
[Motives for the prescription of antibiotics in the emergency department of the University Hospital Center in Nice. A prospective study]. (2002)

Unjustified antibiotherapy in an emergency unit is generally due to erroneous diagnosis of infection. The lack of documentation and inexperience of the prescribers appear to be the two elements contributing to unjustified antibiotherapy.
Heterogeneity of IgG1 monoclonal anti‐Rh(D): an investigation using ADCC and macrophage binding assays (1987)

Three monoclonal IgG1 anti-Rh(D), UCH D4, ARC 7D5 and UKTS FC3, produced by Epstein-Barr virus transformed cells from Rh(D)-sensitized individuals, were compared with polyclonal single donor anti-D sera and therapeutic immunoglobulin preparations in antibody dependent cellular cytotoxicity (ADCC) and macrophage binding tests. When assayed at equal anti-D concentrations monoclonal antibodies varied considerably in their ADCC and macrophage binding activities: only UKTS FC3 showed significant activity in both assays, but these were substantially lower than those of the polyclonal anti-D sera and immunoglobulins. When examined in different combinations the monoclonal antibodies showed little synergism in mediating red cell destruction by the effector cells. Factors which might contribute to the diverse ADCC and macrophage binding activities of the monoclonal anti-Ds of the same IgG subclass are discussed.
PlutoF—a Web Based Workbench for Ecological and Taxonomic Research, with an Online Implementation for Fungal ITS Sequences (2010)

DNA sequences accumulating in the International Nucleotide Sequence Databases (INSD) form a rich source of information for taxonomic and ecological meta-analyses. However, these databases include many erroneous entries, and the data itself is poorly annotated with metadata, making it difficult to target and extract entries of interest with any degree of precision. Here we describe the web-based workbench PlutoF, which is designed to bridge the gap between the needs of contemporary research in biology and the existing software resources and databases. Built on a relational database, PlutoF allows remote-access rapid submission, retrieval, and analysis of study, specimen, and sequence data in INSD as well as for private datasets though web-based thin clients. In contrast to INSD, PlutoF supports internationally standardized terminology to allow very specific annotation and linking of interacting specimens and species. The sequence analysis module is optimized for identification and analysis of environmental ITS sequences of fungi, but it can be modified to operate on any genetic marker and group of organisms. The workbench is available at http://plutof.ut.ee .
Integrating query thesaurus, and documents through a common visual representation (1991)

retrieval is a highly interactive process dealing with large amounts of information.