gebhart/patrepeval huggingface dataset:
Selection of splice sites in pre-mRNAs with short internal exons. (1991)

Model pre-mRNAs containing two introns and three exons, derived from the human beta-globin gene, were used to study the effects of internal exon length on splice site selection. Splicing was assayed in vitro in HeLa nuclear extracts and in vivo during transient expression in transfected HeLa cells. For substrates with internal exons 87, 104, and 171 nucleotides in length, in vitro splicing proceeded via a regular splicing pathway, in which all three exons were included in the spliced product. Primary transcripts with internal exons containing 23, 29, and 33 nucleotides were spliced by an alternative pathway, in which the first exon was joined directly to the third one. The internal exon was missing from the spliced product and together with two flanking introns was included in a large lariat structure. The same patterns of splicing were retained when transcripts containing 171-, 33-, and 29-nucleotide-long internal exons were spliced in vivo. A transcript containing a 51-nucleotide-long exon was spliced in vitro via both pathways but in vivo generated only a correctly spliced product. Skipping of short internal exons was reversed both in vitro and in vivo when purines in the upstream polypyrimidine tract were replaced by pyrimidines. The changes in the polypyrimidine tract achieved by these substitutions led in vitro to complete (transcripts containing 28 pyrimidines in a row) or partial (transcripts containing 15 pyrimidines in a row) restoration of a regular splicing pathway. Splicing in vivo of these transcripts led exclusively to the spliced product containing all three exons. These results suggest that a balance between the length of the uninterrupted polypyrimidine tract and the length of the exon is an important determinant of the relative strength of the splice sites, ensuring correct splicing patterns of multiintron pre-mRNAs.
Purification of the putA gene product. A bifunctional membrane-bound protein from Salmonella typhimurium responsible for the two-step oxidation of proline to glutamate. (1981)

In this paper we report the purification of a protein which is able to catalyze both the proline oxidase and the pyrroline-5-carboxylic acid dehydrogenase activities necessary for the oxidation of proline to glutamic acid. The purification involves the preparation of a crude membrane pellet, detergent solubilization, ammonium sulfate fractionation, and DEAE-chromatography. We are able to obtain an essentially pure preparation (greater than 95% pure) after only a 52-fold purification, demonstrating that the protein is a major protein in cells fully induced for proline utilization. Both proline oxidase and pyrroline-5-carboxylic acid dehydrogenase activities co-purity throughout our purification. Velocity sedimentation of the purified protein demonstrates that both proline oxidase and pyrroline-5-carboxylic acid dehydrogenase activities co-sediment. Early in the purification procedure we are able to detect two species of protein which have both proline oxidase and pyrroline-5-carboxylic acid dehydrogenase activities. Our procedure purifies only the larger molecular weight species. The purified protein is a dimer composed of identical 132,000-dalton subunits. Analysis of mutants defective for proline utilization demonstrate that the bifunctional enzyme is the putA gene product.
IMP3 Expression in Serous Tumors of the Ovary (2014)

Insulin-like growth factor II mRNA-binding protein (IMP3) is an oncofetal protein involved in embryogenesis, which is expressed in a variety of malignant neoplasms. It is rarely expressed in normal adult tissue and benign tumors. The aim of this study was to evaluate the expression of IMP3 in benign and malignant serous tumors of the ovary. Seventy-nine ovarian tumors were examined for IMP3 expression by immunohistochemical analysis, comprising 16 benign serous tumors, 19 borderline serous tumors, and 44 serous carcinomas. Positive staining was defined as brown staining in the cytoplasm. Negative staining was defined as absent staining or staining of <5% of tumor cells. The intensity of staining (weak, moderate, and strong) and percentage (0% to 100%) of neoplastic cells staining positive for cytoplasmic IMP3 staining were recorded in each case. Moderate to strong cytoplasmic staining for IMP3 was observed in 30 of 44 (68%) serous carcinomas of the ovary; in contrast, <5% of the borderline and benign serous tumors expressed IMP3 ranging from weak to strong cytoplasmic staining. Statistically, the difference in IMP3 expression between these groups of tumors was highly significant (P<0.0001). Our findings demonstrate moderate to strong expression of IMP3 in the majority of ovarian serous carcinomas as compared with benign/borderline serous tumors, which demonstrated weak to strong expression in a small minority (<5%) of the tumors. Thus, IMP3 may be a useful adjunctive tool in the pathologic evaluation of ovarian serous tumors.
<title>Complete-to-overcomplete discrete wavelet transforms for scalable video coding with MCTF</title> (2003)

Techniques for full scalability with motion-compensated temporal filtering (MCTF) in the wavelet-domain (in-band) are presented in this paper. The application of MCTF in the wavelet domain is performed after the production of the overcomplete discrete wavelet transform from the critically-sampled decomposition, a process that occurs at both the encoder and decoder side. This process, which is a complete-to-overcomplete discrete wavelet transform, is critical for the efficiency of the system with respect to scalability, coding performance and complexity. We analyze these aspects of the system and set the necessary constraints for drift-free video coding with in-band MCTF. As a result, the proposed architecture permits the independent operation of MCTF within different resolution levels or even different subbands of the transform and allows the successive refinement of the video information in resolution, frame-rate and quality.
T2 Imaging in Monitoring of Intraparenchymal Real-Time Convection-Enhanced Delivery (2011)

Accuracy of cannula placement and initial infusate distribution may be safely determined by saline infusion without significantly altering the subsequent distribution of the tracer agent. T2 RCD provides detection of intraparenchymal convection- enhanced delivery in the uninjured brain and may predict subsequent distribution of a transgene after viral vector infusion.
Optical Instruments and Techniques (1969)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text , "Optical Instruments and Techniques," Appl. Opt. 8, 1062-1062 (1969) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Complex formation between RAS and RAF and other protein kinases. (1993)

We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizosaccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.
Guiding Adult Mammalian Sensory Axons During Regeneration (2008)

Misdirection of axons after nerve injury impairs successful regeneration of adult neurons. Investigations of axon guidance in development have provided an understanding of pathfinding, but their relevance to regenerating adult axons is unclear. We investigated adult mammalian axon guidance during regeneration after peripheral nerve injury and focused on the effects of the prototypic guidance molecule nerve growth factor (NGF). Adult rat sensory neurons from dorsal root ganglia that expressed the NGF receptor tropomyosin-related kinase A (trkA) were presented with a point source of NGF in vitro. Naive trkA neurons had no net turning response to NGF, but if they had been preconditioned by a peripheral nerve transection in vivo before culturing, their growth cones were attracted toward the NGF gradient. A laminin substrate was required for this behavior and an anti-trkA antibody interrupted turning. These data demonstrate that injured adult mammalian axons can be guided as they regenerate. Moreover, despite the downregulation of trkA mRNA and protein levels within the dorsal root ganglion after injury, sensory neurons retain and increase trkA protein at the injury site where the regenerating axons are found. This may enhance the axonal response to NGF and allow guidance along an NGF gradient created in vivo in the distal nerve stump.
Audio transfer system on PHS using error-protected stereo twin VQ (1998)

We propose an efficient stereo audio coding scheme with error protection based on transformed vector quantization. This scheme includes a new stereo coding method, called adaptive inter-channel prediction stereo, that achieves high-efficiency stereo coding at a low coding bit rate. This scheme also includes an unequal error protection (UEP) method that achieves high-efficiency error protection against radio transmission error. We also report a real-time audio transfer system on the Japanese Personal Handyphone System (PHS) using the proposed coding scheme, which enables a high-quality audio transfer service over mobile channels.
Induction of lysosomal membrane permeabilization by compounds that activate p53-independent apoptosis (2004)

The p53 protein activates cellular death programs through multiple pathways. Because the high frequency of p53 mutations in human tumors is believed to contribute to resistance to commonly used chemotherapeutic agents, it is important to identify drugs that induce p53-independent cell death and to define the mechanisms of action of such drugs. Here we screened a drug library (the National Cancer Institute mechanistic set; 879 compounds with diverse mechanisms of actions) and identified 175 compounds that induced caspase cleavage of cytokeratin-18 in cultured HCT116 colon cancer cells at <5 microM. Interestingly, whereas most compounds elicited a stronger apoptotic response in cells with functional p53, significant apoptosis was observed also in p53-null cells. A subset of 15 compounds showing weak or no dependence on p53 for induction of apoptosis was examined in detail. Of these compounds, 11 were capable of activating caspase-3 in enucleated cells. Seven such compounds with nonnuclear targets were found to induce lysosomal membrane permeabilization (LMP). Translocation of the lysosomal proteases cathepsin B and cathepsin D into the cytosol was observed after treatment with these drugs, and apoptosis was inhibited by pepstatin A, an inhibitor of cathepsin D. Apoptosis depended on Bax, suggesting that LMP induced a mitochondrial apoptotic pathway. We conclude that a large number of potential anticancer drugs induce p53-independent apoptosis and that LMP is a mediator of many such responses.
Receptor−G Protein γ Specificity:  γ11 Shows Unique Potency for A1Adenosine and 5-HT1AReceptors (2001)

G protein coupled receptors activate signal transducing guanine nucleotide-binding proteins (G proteins), which consist of an alpha subunit and a betagamma dimer. Whole cell studies have reported that receptors signal through specific betagamma subtypes. Membrane reconstitution studies with the adenosine A(1) and alpha(2A) adrenergic receptors have reached a similar conclusion. We aimed to test the generality of this finding by comparing the gamma subtype specificity for four G(i)-coupled receptors: alpha(2A) adrenergic; A1 adenosine (A(1)-R); 5-hydroxytryptamine(1A) (5-HT(1A)-R); mu opioid. Membranes were reconstituted with Galpha(i)(1) and five gamma subtypes (dimerized to beta1). Using a sensitive alpha-betagamma binding assay, we show that all recombinant betagamma (except beta1gamma1) had comparable affinity for alpha(i)(1). Using high affinity agonist binding as a measure of receptor-G protein coupling, betagamma-containing gamma11 was the most potent for A(1)-R and 5-HT(1A)-R (p < 0.05, one way ANOVA) while gamma7 was most potent for the other two receptors. gamma11 was 3-8-fold more potent for the A(1)-R than were the other gamma subtypes. Also, gamma11 was 2-8-fold more potent for A(1)-R than at the other receptors, suggesting a unique coupling specificity of the A(1)-R for gamma11. In contrast, the discrimination by receptors for the other betagamma subtypes (beta1 and gamma1, gamma2, gamma7, and gamma10) was limited (2-3-fold). Thus the exquisite betagamma specificity of individual receptors reported in whole cell studies may depend on in vivo mechanisms beyond direct receptor recognition of betagamma subtypes.
PAPR REDUCTION IN MIMO SC-FDMA- A SURVEY (2015)

To meet the requirements of high data rate, third generation partnership project (3GPP) has come across the development of long term evolution (LTE). High data rate can be achieved with the help of an advanced modulation method. Orthogonal Frequency Division Multiple Access (OFDMA) was considered as a modulation method especially in the downlink of the communication systems. It gives many advantages like frequency diversity, increased capacity and robustness against impulse noise. Besides these advantages, a major drawback of OFDMA is its high peak-to-average power ratio (PAPR). High PAPR affects the system by lowering the power amplifier efficiency, increased complexity as well as shorter battery life. In case of uplink communication, where better peak power characteristics benefit the mobile terminals with respect to power efficiency, OFDMA is not a possible solution. To handle such situations, 3GPP has introduced a modified form of OFDMA which is known as Single-Carrier Frequency Division Multiple Access (SC-FDMA). It provides same advantages as OFDMA along with low PAPR. To further improve the bit error rate (BER) performance of the system and power amplifier efficiency Multiple-Input Multiple-Output (MIMO) can be extended to SC-FDMA system. This paper surveys causes, effects and possible solutions of high PAPR in a communication system.
Algorithm for machine perception of synthetically significant rings in complex cyclic organic structures (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAlgorithm for machine perception of synthetically significant rings in complex cyclic organic structuresE. J. Corey and George A. PererssonCite this: J. Am. Chem. Soc. 1972, 94, 2, 460–465Publication Date (Print):January 1, 1972Publication History Published online1 May 2002Published inissue 1 January 1972https://doi.org/10.1021/ja00757a023Request reuse permissionsArticle Views177Altmetric-Citations48LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (727 KB) Get e-Alertsclose Get e-Alerts
Spontaneous volume magnetostriction of Dy2AlFe12Mn4 compound (2006)

Dy_2AlFe_(12 ) Mn_4 化合物的结构和磁性借助于 X 光检查衍射和磁化大小被调查了。Dy_2AlFe_(12 ) Mn_4compound 有六角形的 Th_2Ni_(17 ) 类型结构。否定热扩大被 X 光检查 dilatometry 从 229 ～ 280 K 发现 inDy_2AlFe_(12 ) Mn_4 复合。平均热扩大的系数是α =-3.8 x 10 ~(-5)K~(-1) 。从 105 ～ 270 Khave 的 magnetostrictive 变丑借助于格子参数的试验性的价值之间的差别是计算的，相应价值从顺磁的范围外推。结果证明自发的体积 magnetostrictive 变丑ω_ s 从 6.2 x 10 ~减少(接近零，温度从 105～270 K 增加的-3)，沿着 c 轴的自发的线性 magnetostrictivedeformation λ_ c 比自发的线性 magnetostrictivedeformation λ_大得多一在里面在除了到 249 K 的结束的一样的温度基础飞机。
Murine cytotoxic T lymphocyte recognition of individual influenza virus proteins. High frequency of nonresponder MHC class I alleles. (1988)

We determined the MHC restriction of CTL responses to five individual influenza virus proteins. Four viral proteins failed to be recognized in conjunction with three of the five class I alleles of the H-2k and H-2d haplotypes, while the fifth was recognized only in conjunction with a single allele. This indicates that there is a significant chance that a given class I allele will be associated with low responsiveness or nonresponsiveness for a given foreign protein. This explains, at least in part, why MHC-linked nonresponsiveness is frequently detected in polyclonal antiviral CTL responses. Most importantly, these findings support the idea that responsiveness to foreign antigens is a critical factor in maintaining the high degree of MHC class I polymorphism in outbred populations.
Resistance to chemotherapeutic drugs overcome by c-Myc inhibition in a Lewis lung carcinoma murine model (2003)

Chemotherapy resistance is a significant obstacle in lung cancer therapy, and has been found to frequently correlate with amplification and overexpression of the c-myc oncogene. Earlier studies have shown that c-Myc inhibition alone is not always effective in cancer models. The purpose of this study was to test different dosing regimen, which included commonly used chemotherapeutic drugs in combination with c-Myc inhibition in a Lewis lung syngeneic drug-resistant murine tumor model. Inhibition of c-myc was specifically achieved by using phosphorodiamidate Morpholino oligomer (PMOs), a novel, non-toxic antisense DNA chemistry for inhibition of gene expression by an RNase H-independent mechanism. When administration of cisplatin overlapped with c-myc PMO (AVI-4126) treatment there was no additional effect on tumor growth inhibition compared to cisplatin alone. In contrast, using a dosing regimen in which cisplatin or taxol treatment preceded AVI-4126, a dramatic decrease in tumor growth rate was observed with tumor areas less then 0.5 cm2 in 60% of the animals at the end of the study. This effect was specific to c-Myc inhibition as other antisense PMOs against p21 or Rad51 showed no such effect in combination with chemotherapy. Immunoblot and HPLC-based analysis of tumor lysates at the end of the study confirmed c-Myc inhibition and detection of intact AVI-4126, respectively. In conclusion, AVI-4126 potentiates the efficacy of chemotherapeutic drugs in a manner that is schedule dependent.
Photoresponse Properties of CdSe Single‐Nanoribbon Photodetectors (2007)

Abstract Photodetectors are fabricated from individual single‐crystal CdSe nanoribbons, and the photoresponse properties of the devices are studied systematically. The photodetector shows a high sensitivity towards excitation wavelength with a sharp cut‐off at 710 nm, corresponding to the bandgap of CdSe. The device exhibits a high photo‐to‐dark current ratio of five orders of magnitude at 650 nm, and can function with excellent stability, reproducibility, and high response speed (&lt; 1 ms) in a wide range of switching frequency (up to 300 Hz). The photocurrent of the device shows a power‐law dependence on light intensity. This finding together with the analysis of the light intensity‐dependent response speed reveals the existence of various traps at different energy levels (shallow and deep) in the bandgap. Coating with a thin SiO 2 isolating layer increases the photocurrent but decreases the response speed of the CdSe nanoribbon, which is attributed to reduction of recombination centers on ribbon surface.
Synthesis of 2'-0-Alkyloligoribonucleotides (2003)

2′-O-Alkyloligoribonucleotides possess properties that render them ideally suited for studying RNA processing and for the affinity chromatography of RNA-protein complexes. Particularly useful compounds are those in which alkyl is methyl (1–7), ethyl (8), allyl (9), or butyl (10), with most studies being carried out with the methyl and allyl analogs.
Increased incidence of matrix metalloproteinases in urine of cancer patients. (1998)

Matrix metalloproteinases (MMPs) have been implicated in mechanisms of metastasis in experimental cancer models and in human malignancies. In this study, we used substrate gel electrophoresis (zymography) to determine the frequency of detection of MMPs in urine of patients with a variety of cancers. Three molecular weight classes of urinary MMPs, Mr 72,000, Mr 92,000, and high molecular weight (Mr > or = 150,000) species, were detected reproducibly and correlated with disease status. The Mr 72,000 and Mr 92,000 species were identified as MMP-2 and MMP-9, respectively, by Western blot analysis. The presence of biologically active MMP-2 (P < 0.001) or MMP-9 (P = 0.002) was an independent predictor of organ-confined cancer, and the high molecular weight species (P < 0.001) was an independent predictor of metastatic cancer. This is the first study to demonstrate that analysis of urinary MMPs may be useful in determining disease status in a variety of human cancers, both within and outside of the urinary tract.
Glasses with Medical Applications (2003)

Abstract Bioactive glasses react chemically with the body fluids. The reaction product is an apatite, which, with the intervention of biological drivers, assists the generation of bone matrix and bone growth. The main application of these bioactive glasses in the clinical field is the filling of osseous cavities, manufacture of small parts for middle ear bone replacement and maxilofacial reconstruction and dental applications. Thanks to advances in related medical technologies, bioactive glasses are now undergoing a new stage of development, resulting in different mechanical properties, drug delivery capabilities, bioactive coating of metallic implants, protein and/or cell activation for tissue regeneration and tissue engineering, and are also finding use in biomimetics, nanotechnology, production of hybrid organic‐inorganic materials and as bioactivity accelerators of mineral apatites or as bioactivity inductors of magnetic materials for hyperthermia treatment of osseous tumours. (© Wiley‐VCH Verlag GmbH &amp; Co. KGaA, 69451 Weinheim, Germany, 2003)