gebhart/patrepeval huggingface dataset:
Cytokine profile of human adipose‐derived stem cells: Expression of angiogenic, hematopoietic, and pro‐inflammatory factors (2007)

Adipose tissue serves as a source of adipokines and cytokines with both local and systemic actions in health and disease. In this study, we examine the hypothesis that multipotent human adipose-derived stem cells (ASCs), capable of differentiating along the adipocyte, chondrocyte, and osteoblast pathways, contribute to adipose tissue-derived cytokine secretion. Following exposure to basic fibroblast growth factor (bFGF) or epidermal growth factor (EGF), the ASCs significantly increase their secretion of hepatocyte growth factor (HGF), a cytokine implicated in hematopoiesis, vasculogenesis, and mammary epithelial duct formation. Ascorbic acid synergizes with these inductive factors, further increasing HGF levels. Following exposure to lipopolysaccharide, ASCs increase their secretion of both hematopoietic (granulocyte/monocyte, granulocyte, and macrophage colony stimulating factors, interleukin 7) and proinflammatory (interleukins 6, 8, and 11, tumor necrosis factor alpha) cytokines based on ELISA and RT-PCR. In co-cultures established with umbilical cord blood-derived CD34(+) cells, the ASCs support long-term hematopoiesis in vitro. Furthermore, in short-term 12-day co-cultures, the ASC maintain and expand the numbers of both myeloid and lymphoid progenitors. These observations are consistent with the functionality of the secreted cytokines and confirm recent reports by other laboratories concerning the hematopoietic supportive capability of ASCs. We conclude that the ASCs display cytokine secretory properties similar to those reported for bone marrow-derived mesenchymal stem cells (MSCs).
Transforming Growth Factor-β1 Induces Transdifferentiation of Myoblasts into Myofibroblasts via Up-Regulation of Sphingosine Kinase-1/S1P3 Axis (2010)

The pleiotropic cytokine transforming growth factor (TGF)-beta1 is a key player in the onset of skeletal muscle fibrosis, which hampers tissue repair. However, the molecular mechanisms implicated in TGFbeta1-dependent transdifferentiation of myoblasts into myofibroblasts are presently unknown. Here, we show that TGFbeta1 up-regulates sphingosine kinase (SK)-1 in C2C12 myoblasts in a Smad-dependent manner, and concomitantly modifies the expression of sphingosine 1-phosphate (S1P) receptors (S1PRs). Notably, pharmacological or short interfering RNA-mediated inhibition of SK1 prevented the induction of fibrotic markers by TGFbeta1. Moreover, inhibition of S1P(3), which became the highest expressed S1PR after TGFbeta1 challenge, strongly attenuated the profibrotic response to TGFbeta1. Furthermore, downstream of S1P(3), Rho/Rho kinase signaling was found critically implicated in the profibrotic action of TGFbeta1. Importantly, we demonstrate that SK/S1P axis, known to play a key role in myogenesis via S1P(2), consequently to TGFbeta1-dependent S1PR pattern remodeling, becomes responsible for transmitting a profibrotic, antidifferentiating action. This study provides new compelling information on the mechanism by which TGFbeta1 gives rise to fibrosis in skeletal muscle, opening new perspectives for its pharmacological treatment. Moreover, it highlights the pleiotropic role of SK/S1P axis in skeletal myoblasts that, depending on the expressed S1PR pattern, seems capable of eliciting multiple, even contrasting biological responses.
Pulmonary Vein Isolation With Single Pulse Irreversible Electroporation (2020)

In the 10 patients of this first-in-human study, acute bidirectional electrical PV isolation could be achieved safely by single pulse IRE ablation.
Video Question Answering via Hierarchical Spatio-Temporal Attention Networks (2017)

Open-ended video question answering is a challenging problem in visual information retrieval, which automatically generates the natural language answer from the referenced video content according to the question. However, the existing visual question answering works only focus on the static image, which may be ineffectively applied to video question answering due to the temporal dynamics of video contents. In this paper, we consider the problem of open-ended video question answering from the viewpoint of spatio-temporal attentional encoder-decoder learning framework. We propose the hierarchical spatio-temporal attention network for learning the joint representation of the dynamic video contents according to the given question. We then develop the encoder-decoder learning method with reasoning recurrent neural networks for open-ended video question answering. We construct a large-scale video question answering dataset. The extensive experiments show the effectiveness of our method.
Multiple Functions of the Integrin α6β4 in Epidermal Homeostasis and Tumorigenesis (2006)

Click to increase image sizeClick to decrease image size We thank Ed Roos, Anton Berns, Paul Engelfriet, Luca Borradori, Reinhard Fässler, and Johan de Rooij for critical reading of the manuscript.
A Novel Carbohydrate-Based Therapeutic GCS-100 Overcomes Bortezomib Resistance and Enhances Dexamethasone-Induced Apoptosis in Multiple Myeloma Cells (2005)

Human multiple myeloma is a presently incurable hematologic malignancy, and novel biologically based therapies are urgently needed. GCS-100 is a polysaccharide derived from citrus pectin in clinical development for the treatment of cancer. Here we show that GCS-100 induces apoptosis in various multiple myeloma cell lines, including those resistant to dexamethasone, melphalan, or doxorubicin. Examination of purified patient multiple myeloma cells showed similar results. Specifically, GCS-100 decreases viability of bortezomib/PS-341-resistant multiple myeloma patient cells. Importantly, GCS-100 inhibits multiple myeloma cell growth induced by adhesion to bone marrow stromal cells; overcome the growth advantage conferred by antiapoptotic protein Bcl-2, heat shock protein-27, and nuclear factor-kappaB; and blocks vascular endothelial growth factor-induced migration of multiple myeloma cells. GCS-100-induced apoptosis is associated with activation of caspase-8 and caspase-3 followed by proteolytic cleavage of poly(ADP-ribose) polymerase enzyme. Combined with dexamethasone, GCS-100 induces additive anti-multiple myeloma cytotoxicity associated with mitochondrial apoptotic signaling via release of cytochrome c and Smac followed by activation of caspase-3. Moreover, GCS-100 + dexamethasone-induced apoptosis in multiple myeloma cells is accompanied by a marked inhibition of an antiapoptotic protein Galectin-3, without significant alteration in Bcl-2 expression. Collectively, these findings provide the framework for clinical evaluation of GCS-100, either alone or in combination with dexamethasone, to inhibit tumor growth, overcome drug resistance, and improve outcome for patients with this universally fatal hematologic malignancy.
Design considerations for piezoelectric polymer ultrasound transducers (2000)

Much work has been published on the design of ultrasound transducers using piezoelectric ceramics, but a great deal of this work does not apply when using the piezoelectric polymers because of their unique electrical and mechanical properties. The purpose of this paper is to review and present new insight into seven important considerations for the design of active piezoelectric polymer ultrasound transducers: piezoelectric polymer materials selection, transducer construction and packaging requirements, materials characterization and modeling, film thickness and active area design, electroding selection, backing material design, and front protection/matching layer design. Besides reviewing these design considerations, this paper also presents new insight into the design of active piezoelectric polymer ultrasonic transducers. The design and fabrication of an immersible ultrasonic transducer, which has no adhesive layer between the active element and backing layer, is included. The transducer features direct deposition of poly(vinylidene fluoride-trifluoroethylene) [P(VDF-TrFE)] copolymer onto an insulated aluminum backing substrate. Pulse-echo tests indicated a minimum insertion loss of 37 dB and -6 dB bandwidth of 9.8 to 22 MHz (71%). The use of polymer wear-protection/quarter-wave matching layers is also discussed. Test results on a P(VDF-TrFE) transducer showed that a Mylar/sup TM/ front layer provided a slight increase in pulse-echo amplitude of 15% (or 1.2 dB) and an increase in -6 dB pulse-echo fractional bandwidth from 86 to 95%. Theoretical derivations are reported for optimizing the active area of the piezoelectric polymer element for maximum power transfer at resonance. These derivations are extended to the special case for a low profile (i.e., thin) shielded transducer. A method for modeling the non-linear loading effects of a commercial pulser-receiver is also included.
Interaction of fluorescence probes with acetylcholinesterase. Site and specificity of propidium binding (1975)

A bis-quaternary fluorescence probe, propidium diiodide, has been found to exhibit a tenfold enhancement of fluorescence when bound to acetylcholinesterase from Torpedo california. The complex is characterized by a high affinity, KD = 3.0 times 10-7 M, and 1:1 stoichiometry with the 82,000 molecular weight subunit of acetylcholinesterase. A wide variety of other quaternary ammonium ligands such as decamethonium, gallamine, d-tubocurarine, tetraethylammonium, and tetramethylammonium will completely dissociate propidium from the enzyme as will monovalent and divalent inorganic cations. The competitive dissociation does not show cooperative behavior or a distinct, requirement for occupation of multiple sites of different affinity to produce displacement. While a directly competitive relationship can be illustrated macroscopically, the various quaternary ligands show a different susceptibility toward inorganic cation displacement. The affinity of propidium relative to gallamine increases with ionic strength. This finding indicates that there is not complete equivalence in the negative subsites to which quaternary groups bind. Although edrophoniumwill also displace propidium from the enzyme, the dissociation constant obtained from this competitive relationship is 3.5 orders of magnitude greater than the constants obtained for inhibition of catalysis. By competitive displacement titrations it is shown that the primary binding site of edrophonium is distinct from that of propidium and a ternary complex with the two ligands can form on each subunit. In contrast to edrophonium, the binding of propidium is unaffected by methanesulfonylation of the active center serine and is uncompetitive with the carbamylating substrate, N-methyl-7-dimethylcarbamoxyquinolinium. Thus, it appears that propidium associates with a peripheral anionic center on the enzyme. Although propidium and edrophonium associate at separate sites on acetylcholinesterase, bis-quaternary ligands where the quaternary nitrogens are separated by 14 A displace both ligands from the enzyme with equal effectiveness.
Thermal stimulation of electrical activity of single units of the preoptic region (1963)

Single unit activity has been recorded in the hypothalamus and preoptic region of cats anesthetized with urethan during local hypothalamic heating and cooling. Heating was accomplished by radio frequency current (3.7 megacycles/sec) and heating and cooling by circulating warm and cold water through small steel thermodes placed 4–6 mm on each side of the midline. Local tissue temperature was recorded by thermocouples and by thermistors. It was observed from a study of about 1,000 neurons that a) about one neuron in five increased its rate of spontaneous discharge with increase in local temperature from 32 to 41 C (Q 10 = 5–15); b) 80% of the units did not respond to temperature change by changing their spontaneous discharge rate in the 34–41 C temperature range; c) thermally sensitive neurons were found only in the midline region immediately rostroventral to the anterior commissure; d) no units were found for which the discharge frequency increased by decreasing the local temperature.
Robust Tuning of Machine Directional Predictive Control of Paper Machines (2015)

In this work, a parameter tuning problem of two-degrees-of-freedom model predictive control of industrial paper-making processes is explored to achieve satisfactory time-domain robust closed-loop performance in terms of worst-case overshoots and worst-case settling times, under user-specified parametric uncertainties. An efficient visualization method is first developed to characterize the set of time-domain closed-loop responses in the presence of parametric model–plant mismatch. On the basis of the visualization technique and the unmodality/monotonicity properties of the performance indices with respect to the tuning parameters, the feasibility of the tuning problem can be analyzed, and a three-step iterative line-search based automatic tuning algorithm is proposed to determine the controller parameters that meet the time-domain performance requirements robustly for the given parametric uncertainty specifications. The effectiveness of the algorithm is illustrated by applying the results to a process from stock to conditioned weight in an industrial paper machine and by comparing the performance of the algorithm with that of brutal search.
High-brightness slab-coupled optical waveguide laser arrays (2005)

We have constructed high brightness slab-coupled optical waveguide laser arrays. Devices in the array emit in large nearly circular single-spatial modes. We have shown that optical cross-coupling in closely spaced devices is not an issue for maintaining single-mode output, including the effects of slab-coupling. By appropriate heat-sinking, we have shown linear continuous-wave power densities of 98 W/cm along the array emission aperture.
Color and Constitution. X.1 Absorption of the Merocyanines2 (1951)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTColor and Constitution. X.1 Absorption of the Merocyanines2L. G. S. Brooker, G. H. Keyes, R. H. Sprague, R. H. VanDyke, E. VanLare, G. VanZandt, F. L. White, H. W. J. Cressman, and S. G. Dent Jr.Cite this: J. Am. Chem. Soc. 1951, 73, 11, 5332–5350Publication Date (Print):November 1, 1951Publication History Published online1 May 2002Published inissue 1 November 1951https://pubs.acs.org/doi/10.1021/ja01155a096https://doi.org/10.1021/ja01155a096research-articleACS PublicationsRequest reuse permissionsArticle Views976Altmetric-Citations222LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Metal-assisted chemical etching in HF/H2O2 produces porous silicon (2000)

A simple and effective method is presented for producing light-emitting porous silicon (PSi). A thin (d&amp;lt;10 nm) layer of Au, Pt, or Au/Pd is deposited on the (100) Si surface prior to immersion in a solution of HF and H2O2. Depending on the type of metal deposited and Si doping type and doping level, PSi with different morphologies and light-emitting properties is produced. PSi production occurs on the time scale of seconds, without electrical current, in the dark, on both p- and n-type Si. Thin metal coatings facilitate the etching in HF and H2O2, and of the metals investigated, Pt yields the fastest etch rates and produces PSi with the most intense luminescence. A reaction scheme involving local coupling of redox reactions with the metal is proposed to explain the metal-assisted etching process. The observation that some metal remains on the PSi surface after etching raises the possibility of fabricating in situ PSi contacts.
Antibodies targeting cancer stem cells: A new paradigm in immunotherapy? (2009)

Antibody targeting of cancer is showing clinical and commercial success after much intense research and development over the last 30 years. They still have the potential to delivery long-term cures but a shift in thinking towards a cancer stem cell (CSC) model for tumor development is certain to impact on how antibodies are selected and developed, the targets they bind to and the drugs used in combination with them. CSCs have been identified from many human tumors and share many of the characteristics of normal stem cells. The ability to renew, metabolically or physically protect themselves from xenobiotics and DNA damage and the range of locomotory-related receptors expressed could explain the observations of drug resistance and radiation insensitivity leading to metastasis and patient relapse.Targeting CSCs could be a strategy to improve the outcome of cancer therapy but this is not as simple as it seems. Targets such as CD133 and EpCAM/ESA could mark out CSCs from normal cells enabling specific intervention but indirect strategies such as interfering with the establishment of a supportive niche through anti-angiogenic or anti-stroma therapy could be more effective.This review will outline the recent discoveries for CSCs across the major tumor types highlighting the possible molecules for intervention. Examples of antibody-directed CSC therapies and the outlook for the future development of this emerging area will be given.
Cardiac Stem Cells Fail With Aging (2004)

Information about reprints can be found online at: Reprints: document. Permissions and Rights Question and Answer about this process is available in the located, click Request Permissions in the middle column of the Web page under Services. Further information Editorial Office. Once the online version of the published article for which permission is being requested is can be obtained via RightsLink, a service of the Copyright Clearance Center, not theCirculation Researchin Requests for permissions to reproduce figures, tables, or portions of articles originally publishedPermissions: by guest on March 4,
Frequency of Gastrointestinal Symptoms in Children with Autistic Spectrum Disorders and Association with Family History of Autoimmune Disease (2006)

This is a cross-sectional study that compares lifetime prevalence of gastrointestinal (GI) symptoms in children with autistic spectrum disorders (ASDs) and children with typical development and with other developmental disabilities (DDs) and examines the association of GI symptoms with a family history of autoimmune disease. A structured interview was performed in 50 children with ASD and 2 control groups matched for age, sex, and ethnicity-50 with typical development and 50 with other DDs. Seventy-four percent were boys with a mean age of 7.6 years (SD, ±3.6). A history of GI symptoms was elicited in 70% of children with ASD compared with 28% of children with typical development (p <.001) and 42% of children with DD (p =.03). Abnormal stool pattern was more common in children with ASD (18%) than controls (typical development: 4%, p =.039; DD: 2%, p =.021). Food selectivity was also higher in children with ASD (60%) compared with those with typical development (22%, p =.001) and DD (36%, p =.023). Family history of autoimmune disease was reported in 38% of the ASD group and 34% of controls and was not associated with a differential rate of GI symptoms. In the multivariate analysis, autism (adjusted odds ratio (OR), 3.8; 95% confidence interval (CI), 1.7-11.2) and food selectivity (adjusted OR, 4.1; 95% CI, 1.8-9.1) were associated with GI symptoms. Children with ASD have a higher rate of GI symptoms than children with either typical development or other DDs. In this study, there was no association between a family history of autoimmune disease and GI symptoms in children with ASD.
A new pathway for protein export in Saccharomyces cerevisiae. (1996)

Several physiologically important proteins lack a classical secretory signal sequence, yet they are secreted from cells. To investigate the secretion mechanism of such proteins, a representative mammalian protein that is exported by a nonclassical mechanism, galectin-1, has been expressed in yeast. Galectin-1 is exported across the yeast plasma membrane, and this export does not require the classical secretory pathway nor the yeast multidrug resistance-like protein Ste6p, the transporter for the peptide a factor. A screen for components of the export machinery has identified genes that are involved in nonclassical export. These findings demonstrate a new pathway for protein export that is distinct from the classical secretory pathway in yeast.
Transaction Security System (1991)

Components of previous security systems were designed independently from one another and were often difficult to integrate. Described is the recently available IBM Transaction Security System. It implements the Common Cryptographic Architecture and offers a comprehensive set of security products that allow users to implement end-to-end secure systems with IBM components. The system includes a mainframe host-attached Network Security Processor, high-performance encryption adapters for the IBM Personal Computer and Personal System/2® Micro Channel®, an RS-232 attached Security Interface Unit, and a credit-card size state-of-the-art Personal Security™ card containing a high-performance microprocessor. The application programming interface provides common programming in the host and the workstation and supports all of the Systems Application Architecture™ languages except REXX and RPG. Applications may be written to run on Multiple Virtual Storage (MVS) and PC DOS operating systems.
Amorphous silicon solar cells. Quarterly report No. 3, 1 April 1981-30 June 1981 (1981)

DLTS measurements on both Schottky-barrier and p-i-n a-Si:H solar cells reveal a deep electron trap that could be associated with the Staebler-Wronski effect. In one series of experiments, the density of metastable defects was proportional to the amount of oxygen and nitrogen introduced by an air bleed. Infrared absorption studies have shown that recent films produced in an rf magnetron mode are comparable to films produced in rf capacitive or dc proximity modes. Auger electron spectroscopy has been used to show that a-Si:H films deposited on substrates such as ITO are initially patchy and become continuous after about 40 to 50 A of growth. An automated system for measuring diffusion lengths is now operational and values as large as 0.47 ..mu..m have been measured in a-Si:H under 1-sun light bias. The Hall effect in p-type a-Si:H (0.4 at. % boron) exhibits the correct sign for temperatures <100/sup 0/C but reverses for high temperatures, thus indicating two hole conduction modes. Conversion efficiencies up to 5.2% have been obtained for p-i-n cells with a microcrystalline p layer. Open-circuit voltages as large as 933 mV have been observed in p-i-n structures with carbon-containing p layers. Diffusion length measurements have been shown to bemore » very sensitive to degradation mechanisms such as electron bombardment.« less