gebhart/patrepeval huggingface dataset:
Role of the glycoprotein Ib-binding A1 repeat and the RGD sequence in platelet adhesion to human recombinant von Willebrand factor (1995)

To assess the relative importance of the glycoprotein (GP) Ib binding domain and the RGDS binding site in platelet adhesion to isolated von Willebrand factor (vWF) and to collagen preincubated with vWF, we deleted the A1 domain yielding delta A1-vWF and introduced an aspartate- to-glycine substitution in the RGDS sequence by site-directed mutagenesis (RGGS-vWF). Recombinant delta A1-vWF and RGGS-vWF, purified from transfected baby hamster kidney cells, were compared with recombinant wild-type vWF (WT-vWF) in platelet adhesion under static and flow conditions. Purified mutants were coated on glass or on a collagen type III surface and exposed to circulating blood in a perfusion system. Platelet adhesion under static condition, under flow conditions, and in vWF-dependent adhesion to collagen has an absolute requirement for GPIb-vWF interaction. The GPIIb/IIIa-vWF interaction is required for adhesion to coated vWF under flow conditions. Under static condition and vWF-dependent adhesion to collagen, platelet adhesion to RGGS-vWF is similar as to WT-vWF, but platelet spreading and aggregation are abolished.
How treatment priorities influence triptan preferences in clinical practice: perspectives of migraine sufferers, neurologists, and primary care physicians (2005)

Almotriptan, eletriptan, and rizatriptan were the three triptans closest to the ideal, from the perspectives of migraine sufferers, PCPs, and neurologists alike. The TRIPSTAR model may be a potentially useful decision-support tool to help physicians select the triptan most likely to produce a successful outcome in migraine sufferers.
Crystal structure of recombinant human tissue kallikrein at 2.0 Å resolution (1998)

Human tissue kallikrein, a trypsin-like serine protease involved in blood pressure regulation and inflammation processes, was expressed in a deglycosylated form at high levels in Pichia pastoris, purified, and crystallized. The crystal structure at 2.0 A resolution is described and compared with that of porcine kallikrein and of other trypsin-like proteases. The active and S1 sites (nomenclature of Schechter I, Berger A, 1967, Biochem Biophys Res Commun 27:157-162) are similar to those of porcine kallikrein. Compared to trypsin, the S1 site is enlarged owing to the insertion of an additional residue, cis-Pro 219. The replacement Tyr 228 --> Ala further enlarges the S1 pocket. However, the replacement of Gly 226 in trypsin with Ser in human tissue kallikrein restricts accessibility of substrates and inhibitors to Asp 189 at the base of the S1 pocket; there is a hydrogen bond between O delta1Asp189 and O gammaSer226. These changes in the architecture of the S1 site perturb the binding of inhibitors or substrates from the modes determined or inferred for trypsin. The crystal structure gives insight into the structural differences responsible for changes in specificity in human tissue kallikrein compared with other trypsin-like proteases, and into the structural basis for the unusual specificity of human tissue kallikrein in cleaving both an Arg-Ser and a Met-Lys peptide bond in its natural protein substrate, kininogen. A Zn+2-dependent, small-molecule competitive inhibitor of kallikrein (Ki = 3.3 microM) has been identified and the bound structure modeled to guide drug design.
Significance of CD44 and CD24 as Cancer Stem Cell Markers: An Enduring Ambiguity (2012)

Cancer stem cell population is a subset of cells capable of dictating invasion, metastasis, heterogeneity, and therapeutic resistance in tumours. Eradication of this rare population is a new insight in cancer treatment. However, prospective identification, characterization, and isolation of these CSCs have been a major challenge. Many studies were performed on surface markers for potential identification and isolation of CSCs. Lack of universal expression of surface markers limits their usage and no best combination of markers has yet been confirmed to identify CSCs capable of initiating and metastasizing tumours. CD44, a hyaluronic acid receptor, is one of the most commonly studied surface markers, which is expressed by almost every tumour cell. CD24, a heat stable antigen, is another surface marker expressed in many tumour types. However, their expression and prognostic value in isolating CSCs are still an enduring ambiguity. In this critical review, we assess the role of CD44 and CD24 in tumour initiation, development, and metastasis. We mainly focus on analysing the significance of CD44 and CD24 as CSC surface markers in combination or with other putative markers in different types of cancer.
Biomaterials Engineering and Devices: Human Applications (2000)

and to describe generally accepted practices. The contributors herein have carefully checked to ensure that the drug selections and dosages set forth in this text are accurate and in accord with the standards accepted at the time of publication. Notwithstanding, as new research, changes in government regulations, and knowledge from clinical experience relating to drug therapy and drug reactions constantly occurs, the reader is advised to check the product information provided by the manufacturer of each drug for any change in dosages or for additional warnings and contraindications. This is of utmost importance when the recommended drug herein is a new or infrequently used drug. It is the responsibility of the treating physician to determine dosages and treatment strategies for individual patients. Further, it is the responsibility of the health care provider to ascertain the Food and Drug Administration status of each drug or device used in their clinical practice. The publisher, editors, and authors are not responsible for errors or omissions or for any consequences from the application of the information presented in this book and make no warranty, express or implied, with respect to the contents in this publication.
Temperature-insensitive fiber Bragg grating force sensor via a bandwidth modulation and optical-power detection technique (2006)

A technique for temperature-insensitive force measurement using a single fiber Bragg grating (FBG) based on strain-gradient-induced reflection spectrum-bandwidth modulation and optical-power detection is demonstrated. A specially designed bending cantilever beam (BCB) is used to induce axial-strain gradient along the sensing FBG, resulting in a Bragg bandwidth modulation. The broadening of the FBG spectrum bandwidth and reflection optical power linearly change with the applied force, and both of them are insensitive to spatially uniform temperature variations. For a temperature range from 20 degC to 80 degC, a linear response of force measurement up to 20 N with fluctuation less than 0.8% full-scale is achieved without any temperature compensation. The demodulation process is simplified by optical-power detection via a p-i-n photodiode, and the sensing system is potentially cost-effective
Cg (2003)

The latest real-time graphics architectures include programmable floating-point vertex and fragment processors, with support for data-dependent control flow in the vertex processor. We present a programming language and a supporting system that are designed for programming these stream processors. The language follows the philosophy of C, in that it is a hardware-oriented, general-purpose language, rather than an application-specific shading language. The language includes a variety of facilities designed to support the key architectural features of programmable graphics processors, and is designed to support multiple generations of graphics architectures with different levels of functionality. The system supports both of the major 3D graphics APIs: OpenGL and Direct3D. This paper identifies many of the choices that we faced as we designed the system, and explains why we made the decisions that we did.
Huntingtin Phosphorylation Sites Mapped by Mass Spectrometry (2006)

Huntingtin (Htt) is a large protein of 3144 amino acids, whose function and regulation have not been well defined. Polyglutamine (polyQ) expansion in the N terminus of Htt causes the neurodegenerative disorder Huntington disease (HD). The cytotoxicity of mutant Htt is modulated by proteolytic cleavage with caspases and calpains generating N-terminal polyQ-containing fragments. We hypothesized that phosphorylation of Htt may modulate cleavage and cytotoxicity. In the present study, we have mapped the major phosphorylation sites of Htt using cell culture models (293T and PC12 cells) expressing full-length myc-tagged Htt constructs containing 23Q or 148Q repeats. Purified myc-tagged Htt was subjected to mass spectrometric analysis including matrix-assisted laser desorption/ionization mass spectrometry and nano-HPLC tandem mass spectrometry, used in conjunction with on-target alkaline phosphatase and protease digestions. We have identified more than six novel serine phosphorylation sites within Htt, one of which lies in the proteolytic susceptibility domain. Three of the sites have the consensus sequence for ERK1 phosphorylation, and addition of ERK1 inhibitor blocks phosphorylation at those sites. Other observed phosphorylation sites are possibly substrates for CDK5/CDC2 kinases. Mutation of amino acid Ser-536, which is located in the proteolytic susceptibility domain, to aspartic acid, inhibited calpain cleavage and reduced mutant Htt toxicity. The results presented here represent the first detailed mapping of the phosphorylation sites in full-length Htt. Dissection of phosphorylation modifications in Htt may provide clues to Huntington disease pathogenesis and targets for therapeutic development.
Parkinsonian signs in older people (2003)

The reduced risk of parkinsonian signs in cigarette smokers could reflect a protective effect of smoking on age-related parkinsonian signs in the elderly or an aversion to smoking in elderly persons with mild parkinsonism.
Molecular Cloning and mRNA Expression of Geraniol-inducible Genes in Cultured Shoot Primordia ofMatricaria chamomilla (2002)

Genes for two geraniol-responsive factors, designated McEREBP1 and McWRKY1, from cultured shoot primordia of Matricaria chamomilla were cloned. The deduced amino acid sequences of these genes were highly similar to those of the family of ethylene-responsive element binding proteins and elicitor-induced DNA-binding proteins containing a WRKY domain, respectively. The levels of McEREBP1 and McWRKY1 mRNAs were maximum when measured 1 h after treatment of the cultured cells with geraniol.
Sialyl Lewisxhybridized complement receptor type 1 moderates acid aspiration injury (2001)

The potentially enhanced anti-inflammatory effects of the sialyl Lewis(x) (sLe(x))-decorated version of soluble complement receptor type 1 (sCR1) in moderating acid aspiration injury are examined. HCl was instilled in tracheostomy tubes placed in mice, and extravasation of (125)I-labeled albumin in bronchoalveolar lavage (BAL) fluid was used to calculate the vascular permeability index (PI). Neutrophil counts in BAL fluid and immunohistochemistry were performed. PI was moderated by 82% after treatment with sCR1sLe(x) compared with 54% in sCR1-untreated mice (P < 0.05). Respective reductions in PI in mice treated 0.5 and 1 h after acid aspiration with sCR1sLe(x) of 70 and 57% were greater than the decreases in PI of 45 and 38% observed in respective sCR1-treated groups (P < 0.05). BAL fluid neutrophil counts in sCR1sLe(x)-treated mice were significantly less than those in sCR1-treated animals, which were similar to those in untreated mice. Immunohistochemistry stained for sCR1 only on the pulmonary vascular endothelium of sCR1sLe(x)- but not sCR1-treated mice. In conclusion, sCR1sLe(x) moderates permeability by antagonizing complement activation and neutrophil adhesion. Delayed complement and neutrophil antagonism significantly reduces injury.
Seeded Growth of Highly Luminescent CdSe/CdS Nanoheterostructures with Rod and Tetrapod Morphologies (2007)

We have demonstrated that seeded growth of nanocrystals offers a convenient way to design nanoheterostructures with complex shapes and morphologies by changing the crystalline structure of the seed. By using CdSe nanocrystals with wurtzite and zinc blende structure as seeds for growth of CdS nanorods, we synthesized CdSe/CdS heterostructure nanorods and nanotetrapods, respectively. Both of these structures showed excellent luminescent properties, combining high photoluminescence efficiency (approximately 80 and approximately 50% for nanorods and nanotetrapods, correspondingly), giant extinction coefficients (approximately 2 x 10(7) and approximately 1.5 x 10(8) M(-1) cm(-1) at 350 nm for nanorods and nanotetrapods, correspondingly), and efficient energy transfer from the CdS arms into the emitting CdSe core.
Creating volume models from edge-vertex graphs (1982)

The design of complex geometric models has been and will continue to be one of the limiting factors in computer graphics. A careful enumeration of the properties of topologically correct models, so that they may be automatically enforced, can greatly speed this process. An example of the problems inherent in these methods is the “wire frame” problem, the automatic generation of a volume model from an edge-vertex graph. The solution to this problem has many useful applications in geometric modelling and scene recognition. This paper shows that the “wire frame” problem is equivalent to finding the embedding of a graph on a closed orientable surface. Such an embedding satisfies all the topological properties of physical volumes. Unfortunately graphical embeddings are not necessarily unique. But when we restrict the embedding surface so that it is equivalent to a sphere, and require that the input graph be three-connected, the resulting object is unique. Given these restrictions there exists a linear time algorithm to automatically convert the “wire frame” to the winged edge representation, a very powerful data structure. Applications of this algorithm are discussed and several examples shown.
Development of the Primary Antibody Repertoire (1987)

The ability to generate a diverse immune response depends on the somatic assembly of genes that encode the antigen-binding portions of immunoglobulin molecules. In this article, we discuss the mechanism and control of these genomic rearrangement events and how aspects of this process are involved in generating the primary antibody repertoire.
Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis. (1975)

Closed-circular, superhelical DNA from simian virus 40 (SV40 DNA I) was treated with an excess of DNA-relaxing enzyme in the presence of increasing amounts of ethidium bromide (EtdBr). After removal of the ethidium, each sample consisted of a group of close-circular DNA molecules differing in their number of superhelical turns (tau) around a mean value of tau in a Gaussian-like distribution. The DNA samples were analyzed by electrophoresis in agarose gels under conditions where the electrophoretic mobility was a function of the number of superhelical turns. Since the distributions around tau of DNA molecules of different samples overlappped, the difference in the mean number of superhelical turns from sample to sample, delta tau, could be determined and used to measure the mean number (tau) for native SV40 DNA I. By this criterion, SV40 DNA I contains a Gaussian-like distribution of molecules differing by integral numbers around a mean value of tau = -24 +/- 2 at 37 degree C [in 0.2 M NaC1, 10 mM Tris-HC1 (pH 7.9), and 0.2 mM EDTA]. The heterogeneity in tau is probably a consequence of thermal fluctuations in the DNA helix at the time when the last phospholiester bond is closed in vivo. When correlated to the buoyant shift of completely relaxed SV40 DNA in a CsC1-propidium diiodide gradient, the number of delta tau = 24 +/- 2 of superhelical versus relaxed DNA implies an unwinding of the DNA helix by 26-28 degree upon intercalation of one molecule of EtdBr.
Cutting Edge: Autocrine TGF-β Sustains Default Tolerogenesis by IDO-Competent Dendritic Cells (2008)

CD8(-) and CD8(+) dendritic cells (DCs) are distinct subsets of mouse splenic accessory cells with opposite but flexible programs of Ag presentation, leading to immunogenic and tolerogenic responses, respectively. In this study, we show that the default tolerogenic function of CD8(+) DCs relies on autocrine TGF-beta, which sustains the activation of IDO in response to environmental stimuli. CD8(-) DCs do not produce TGF-beta, yet externally added TGF-beta induces IDO and turns those cells from immunogenic into tolerogenic cells. The acquisition of a suppressive phenotype by CD8(-) DCs correlates with activation of the PI3K/Akt and noncanonical NF-kappaB pathways. These data are the first to link TGF-beta signaling with IDO in controlling spontaneous tolerogenesis by DCs.
Deep Image Homography Estimation (2016)

We present a deep convolutional neural network for estimating the relative homography between a pair of images. Our feed-forward network has 10 layers, takes two stacked grayscale images as input, and produces an 8 degree of freedom homography which can be used to map the pixels from the first image to the second. We present two convolutional neural network architectures for HomographyNet: a regression network which directly estimates the real-valued homography parameters, and a classification network which produces a distribution over quantized homographies. We use a 4-point homography parameterization which maps the four corners from one image into the second image. Our networks are trained in an end-to-end fashion using warped MS-COCO images. Our approach works without the need for separate local feature detection and transformation estimation stages. Our deep models are compared to a traditional homography estimator based on ORB features and we highlight the scenarios where HomographyNet outperforms the traditional technique. We also describe a variety of applications powered by deep homography estimation, thus showcasing the flexibility of a deep learning approach.
Purification and properties of an iron‐sulfur‐containing and pyridoxal‐phosphate‐independent l‐Serine dehydratase from Peptostreptococcus asaccharolyticus (1991)

L-Serine dehydratase with a specific activity of 15 nkat/mg protein was present in the anaerobic eubacterium Peptostreptococcus asaccharolyticus grown either on L-glutamate or L-serine. The enzyme was highly specific for L-serine with the lowest Km = 0.8 mM ever reported for an L-serine dehydratase. L-Threonine (Km = 22 mM) was the only other substrate. V/Km for L-serine was 500 times higher than that for L-threonine. L-Cysteine was the best inhibitor (Ki = 0.3 mM, competitive towards L-serine). The enzyme was purified 400-fold to homogeneity under anaerobic conditions (specific activity 6 mukat/mg). PAGE in the presence of SDS revealed two subunits with similar intensities (alpha, 30 kDa; beta, 25 kDa). The molecular mass of the native enzyme was estimated as 200 +/- 20 kDa (gel filtration) and 180 kDa (gradient PAGE). In the absence of oxygen the enzyme was moderately stable even in the presence of sodium borohydride or phenylhydrazine (5 mM each). However, by exposure to air the activity was lost, especially when the latter agent was added. The enzyme was reactivated by ferrous ion under anaerobic conditions. The inability of several nucleophilic agents to inactivate the enzyme indicated the absence of pyridoxal phosphate. This was confirmed by a microbiological determination of pyridoxal phosphate. However, the enzyme contained 3.8 +/- 0.2 mol Fe and 5.6 +/- 0.3 mol inorganic sulfur/mol heterodimer (55 kDa) indicating the presence of an [Fe-S] center. The enzyme was successfully applied to measure L-serine concentrations in bacterial media and in human sera.
Lyme Disease (2001)

Since its original description nearly 25 years ago,1 Lyme disease has become recognized as an important infectious disease in the United States. The infection, which is caused by the tick-borne spirochete Borrelia burgdorferi, is now endemic in more than 15 states and has been responsible for focal outbreaks in some eastern coastal areas. Lyme borreliosis is also endemic in Europe and Asia, where certain aspects of the disease (erythema migrans, meningopolyneuritis, and acrodermatitis chronica atrophicans) were described in the early and mid-20th century. These syndromes were linked conclusively in 1982 and 1983 with the recovery of a previously unrecognized spirochete . . .
The Influence of Culture Medium and Light Cycle on the Productivity of the Green Algae Neochloris Oleoabundans (2010)

Biofuels from algae are a promising source of alternative energy. One algae species, Neochloris oleoabundans, shows potential for successful biodiesel production, where biodiesel is produced from the neutral lipid content of the algae. Algal neutral lipid content may be influenced and increased by changes in the surrounding environment. One possible way to influence lipid synthesis is through the growth medium. Two published growth mediums were compared using photobioreactors. Two experimental runs were performed with each medium: 1) continuous light and 2) 12 hour on, 12 hour off light cycle. In addition to medium composition, the effect of light cycle on kinetics and energy content was investigated.
BF3·OEt2-Mediated Rearrangement of Cyclopropyl Carbinols:  A Concise Route to Polycyclic Cyclobutanes (2001)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTBF3·OEt2-Mediated Rearrangement of Cyclopropyl Carbinols: A Concise Route to Polycyclic CyclobutanesChristophe Hardouin, Frédéric Taran, and Eric DorisView Author Information CEA/Saclay, Service des Molécules Marquées, Bât. 547, Département de Biologie Cellulaire et Moléculaire, 91191 Gif sur Yvette Cedex, France [email protected]Cite this: J. Org. Chem. 2001, 66, 12, 4450–4452Publication Date (Web):May 22, 2001Publication History Received23 February 2001Published online22 May 2001Published inissue 1 June 2001https://pubs.acs.org/doi/10.1021/jo0102056https://doi.org/10.1021/jo0102056brief-reportACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views652Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Cations,Hydrocarbons,Rearrangement Get e-Alerts
Two Stage Narrowband Interference Mitigation for Transmitted Reference UWB Systems (2007)

Transmitted Reference (TR) UWB using an autocorrelation receiver (AcR) provides a simple architecture and yet robust functionality against multipath propagation. Narrowband interference (NBI), however, can easily jam and saturate the AcR front-end and degrade the performance severely. To cope with this problem, we propose a front-end NBI cancellation technique followed by a linear combining stage. The two-stage mitigation scheme allows the receiver to cope with low SIR scenarios. It provides superior performance than what can be achieved with either of the techniques alone. Computer simulation is conducted in the presence of an IEEE802.11a WLAN service with a random center frequency in the ranges of 5.15-5.875 GHz.