gebhart/patrepeval huggingface dataset:
Ionizing radiation sensitizes erythroleukemic cells but not normal erythroblasts to tumor necrosis factor–related apoptosis-inducing ligand (TRAIL)–mediated cytotoxicity by selective up-regulation of TRAIL-R1 (2001)

Abstract Cytotoxic activity of tumor necrosis factor–related apoptosis-inducing ligand (TRAIL/Apo-2 ligand), used alone or in different combinations with either a low (1.5 Gy) or a high (15 Gy) single dose of ionizing radiation (IR), was investigated on erythroleukemic cells (K562, HEL, Friend, primary leukemic erythroblasts) and on primary CD34+-derived normal erythroblasts. Human recombinant TRAIL alone variably affected the survival/growth of erythroleukemic cells; K562 cells were the most sensitive. Moreover, all erythroleukemic cells were radio-resistant, as demonstrated by the fact that cytotoxicity was evident only after treatment with high-dose (15 Gy) IR. Remarkably, when IR and TRAIL were used in combination, an additive effect was noticed in all erythroleukemic cells. Augmentation of TRAIL-induced cell death by IR was observed with both low and high IR doses and required the sequential treatment of IR 3 to 6 hours before the addition of TRAIL. Conversely, both TRAIL and IR showed a moderate cytotoxicity on primary CD34+-derived normal erythroblasts when used alone, but their combination did not show any additive effect. Moreover, the cytotoxicity of IR plus TRAIL observed in erythroleukemic cells was accompanied by the selective up-regulation of the surface expression of TRAIL-R1 (DR4), and it was completely blocked by the z-Val-Ala-Asp (OMe)-CH2 (z-VAD-fmk) caspase inhibitor. On the other hand, the surface expression of TRAIL-R1 in CD34+-derived normal erythroblasts was unaffected by IR, which induced the up-regulation of the decoy TRAIL-R3. These data demonstrate that treatment with IR provides an approach to selectively sensitize erythroleukemic cells, but not normal erythroblasts, to TRAIL-induced apoptosis through the functional up-regulation of TRAIL-R1.
Tomographical imaging using uniformly redundant arrays (1979)

Recent work in coded aperture imaging has shown that the uniformly redundant array (URA) can image distant planar radioactive sources with no artifacts. This paper investigates the performance of two URA apertures when used in a close-up tomographic imaging system. It is shown that a URA based on m sequences is superior to one based on quadratic residues. The m-sequence array not only produces less noticeable defocus artifacts in tomographic imaging but is also more resilient to some described detrimental effects of close-up imaging. It is shown that, in spite of these close-up effects, the URA system retains tomographic depth resolution even as the source is moved close to the detector. The URAs based on m sequences provide better images than those obtained using random arrays. This compliments previous studies that have shown random arrays to have better tomographical properties than Fresnel zone plates and nonredundant arrays.
Feline muscular dystrophy with dystrophin deficiency. (1989)

This is the first description of a dystrophin-deficient muscular dystrophy in domestic cats. The disorder appears to be of X-linked inheritance because it affected both males of a litter of four kittens. Immunoblotting and immunofluorescent detection of dystrophin showed dystrophin present in control cat muscle but no detectable dystrophin in either affected cat. The feline muscular dystrophy was progressive and histopathologically resembled human Duchenne/Becker muscular dystrophy except for the lack of fat infiltration and the presence of prominent hypertrophy of both muscle fibers and muscles groups in the feline disorder.
Morbilidad por cáncer. Provincia de Matanzas 2001. (2003)

The cancer constitutes a problem of health in the countries developed as second cause of death. In the second half of the century, the countries of Latin America and the Caribbean they have experienced demographic and epidemic changes that have taken to an increment of the non transmissible illnesses, as this. We motivate ourselves to carry out this study with the objective of determining the behaviour of the morbidity by cancer in the county of Matanzas in the year 2001. He/she was carried out an observational epidemic study of descriptive type with the objective of determining the behaviour of the morbidity by cancer in the county of Matanzas in the year 2001. Summary measures were used for qualitative data, you calculate absolute and percents, the results were presented in charts and graphics for the best analysis and interpretation of the same ones. A discreet bigger incidence is presented in the feminine sex with a frank prevalence of the group of 65 year-old age and more. The Carcinoma was the diagnosis morphologic of more incidence. In more than half of the cases the morphologic differences could not be determined prevailing in those carried out the one Differed. The histology diagnosis prevailed with a poor diagnosis cytology. By the biggest percent in the cases you could not determine the clinical stage, in the cases in that he/she was carried out the stage it prevailed I. The clinical extension of more incidence it was the one located.
Enhancement of sample loadings for the analysis of oligosaccharides isolated from Pseudomonas aeruginosa using transient isotachophoresis and capillary zone electrophoresis—electrospray—mass spectrometry (1998)

The analysis of underivatized core oligosaccharides arising from mild acid hydrolysis of lipopolysaccharides from Pseudomonas aeruginosa serotype 05 was achieved using a transient isotachophoretic preconcentration method coupled to capillary zone electrophoresis-electrospray-mass spectrometry (tCITP-CZE-ES-MS). The combination of a tCITP preconcentration step provided a 10- to 50-fold enhancement of sample loading and a corresponding improvement in sensitivity compared to the conventional zone electrophoresis format. Electrophoretic conditions, enabling the separation of these anionic analytes, were developed to determine possible sites of heterogeneity on either the core or the O-chain structures. The tCITP-CZE-ES-MS technique provided unparalleled resolution of the different core glycoforms and oligosaccharides obtained from the acid cleavage of the native endotoxins whether isolated following conventional gel permeation chromatography or obtained from direct hydrolysis of the bacterial isolates. These investigations also highlighted the highly phosphorylated nature of these complex cell membrane components, where the heptose residues of the core oligosaccharide can bear up to six phosphate groups.
Targeting of the 67-kDa Isoform of Glutamic Acid Decarboxylase to Intracellular Organelles Is Mediated by Its Interaction with the NH2-terminal Region of the 65-kDa Isoform of Glutamic Acid Decarboxylase (1995)

The two isoforms of glutamic acid decarboxylase (GAD), GAD67 and GAD65, synthesize the neurotransmitter gamma-aminobutyric acid in neurons and pancreatic beta-cells. Previous studies suggest that GAD67 is a soluble cytosolic protein, whereas GAD65 is membrane-associated. Here, we study the intracellular distribution of GAD67 in neurons, pancreatic beta-cells, and fibroblasts transfected either with GAD65 and GAD67 together or with GAD67 alone. Neuronal GAD67 is partially recovered with GAD65 in membrane-containing pellet fractions and Triton X-114 detergent phases. The two proteins co-immunoprecipitate from extracts of brain and GAD65-GAD67 co-transfected fibroblasts, but not when extracts of GAD65 and GAD67 transfected fibroblasts were mixed and used as a starting material for immunoprecipitation. GAD67 is concentrated in the Golgi complex region in GAD65-GAD67 co-transfected fibroblasts, but not in fibroblasts transfected with GAD67 alone. A pool of neuronal GAD67 co-localizes with GAD65 in the Golgi complex region and in many synapses. The two proteins also co-localize in the perinuclear region of some pancreatic beta-cells. GAD67 interacts with the NH2-terminal region of GAD65, even in the absence of palmitoylation of this region of GAD65. Taken together, our results indicate that GAD65-GAD67 association occurs in vivo and is required for the targeting of GAD67 to membranes.
Catalytic Enantioselective C−H Activation by Means of Metal−Carbenoid-Induced C−H Insertion (2003)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCatalytic Enantioselective C−H Activation by Means of Metal−Carbenoid-Induced C−H InsertionHuw M. L. Davies and Rohan E. J. BeckwithView Author Information Department of Chemistry, State University of New York at Buffalo, Buffalo, New York 14260-3000 Cite this: Chem. Rev. 2003, 103, 8, 2861–2904Publication Date (Web):June 11, 2003Publication History Received3 February 2003Published online11 June 2003Published inissue 1 August 2003https://pubs.acs.org/doi/10.1021/cr0200217https://doi.org/10.1021/cr0200217research-articleACS PublicationsCopyright © 2003 American Chemical SocietyRequest reuse permissionsArticle Views25038Altmetric-Citations1498LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Carbene compounds,Catalysts,Chemical reactions,Stereoselectivity,Substituents Get e-Alerts
Inhibition of autophagy through MAPK14-mediated phosphorylation of ATG5 (2012)

Autophagy is a catabolic mechanism that is important for many biological processes such as cell homeostasis, development and immunity. Though many molecular components of the autophagy pathway have been identified, the signaling pathways regulating the activity of essential autophagy mediators are still poorly defined. We recently demonstrated that the mitogen-activated protein kinase MAPK14 (p38α), when activated by the GADD45B (Gadd45β)-MAP3K4 (MEKK4) signaling complex (but not other MAPK14 activators), is directed to autophagosomes. Therefore, we demonstrated for the first time that MAPK14 operates at this subcellular compartment. Importantly, activation of MAPK14 impairs autophagosome-lysosome fusion and, thus, autophagy. This was demonstrated by increased autophagic flux in MAPK14-deficient as well as in GADD45B-deficient cells. Moreover, we identified a novel post-translational modification of the crucial autophagy mediator ATG5, since MAPK14 directly phosphorylates ATG5 at threonine 75, which is evolutionarily conserved from yeast to human. Using ATG5-deficient cells, which we reconstituted with either a phosphorylation-defective or a phospho-mimetic mutant of ATG5, we demonstrated that phosphorylation of ATG5 results in impaired autophagy.
SCD1 Inhibition Causes Cancer Cell Death by Depleting Mono-Unsaturated Fatty Acids (2012)

Increased metabolism is a requirement for tumor cell proliferation. To understand the dependence of tumor cells on fatty acid metabolism, we evaluated various nodes of the fatty acid synthesis pathway. Using RNAi we have demonstrated that depletion of fatty-acid synthesis pathway enzymes SCD1, FASN, or ACC1 in HCT116 colon cancer cells results in cytotoxicity that is reversible by addition of exogenous fatty acids. This conditional phenotype is most pronounced when SCD1 is depleted. We used this fatty-acid rescue strategy to characterize several small-molecule inhibitors of fatty acid synthesis, including identification of TOFA as a potent SCD1 inhibitor, representing a previously undescribed activity for this compound. Reference FASN and ACC inhibitors show cytotoxicity that is less pronounced than that of TOFA, and fatty-acid rescue profiles consistent with their proposed enzyme targets. Two reference SCD1 inhibitors show low-nanomolar cytotoxicity that is offset by at least two orders of magnitude by exogenous oleate. One of these inhibitors slows growth of HCT116 xenograft tumors. Our data outline an effective strategy for interrogation of on-mechanism potency and pathway-node-specificity of fatty acid synthesis inhibitors, establish an unambiguous link between fatty acid synthesis and cancer cell survival, and point toward SCD1 as a key target in this pathway.
Understanding the 3D layout of a cluttered room from multiple images (2014)

We present a novel framework for robustly understanding the geometrical and semantic structure of a cluttered room from a small number of images captured from different viewpoints. The tasks we seek to address include: i) estimating the 3D layout of the room - that is, the 3D configuration of floor, walls and ceiling; ii) identifying and localizing all the foreground objects in the room. We jointly use multiview geometry constraints and image appearance to identify the best room layout configuration. Extensive experimental evaluation demonstrates that our estimation results are more complete and accurate in estimating 3D room structure and recognizing objects than alternative state-of-the-art algorithms. In addition, we show an augmented reality mobile application to highlight the high accuracy of our method, which may be beneficial to many computer vision applications.
Auction Design for Standard Offer Service (1997)

During the transition to a competitive electricity market, when a consumer does not select an electricity provider, who provides service to the customer and at what price? An auction for this "standard offer service" is a market-based way to assign the service responsibility and to determine its price. We explore the design issues in establishing rules for such an auction.
Optical measurement of cerebral hemodynamics and oxygen metabolism in neonates with congenital heart defects (2010)

We employ a hybrid diffuse correlation spectroscopy (DCS) and near-infrared spectroscopy (NIRS) monitor for neonates with congenital heart disease (n=33). The NIRS-DCS device measured changes during hypercapnia of oxyhemoglobin, deoxyhemoglobin, and total hemoglobin concentrations; cerebral blood flow (rCBF(DCS)); and oxygen metabolism (rCMRO(2)). Concurrent measurements with arterial spin-labeled magnetic resonance imaging (rCBF(ASL-MRI), n=12) cross-validate rCBF(DCS) against rCBF(ASL-MRI), showing good agreement (R=0.7, p=0.01). The study demonstrates use of NIRS-DCS on a critically ill neonatal population, and the results indicate that the optical technology is a promising clinical method for monitoring this population.
Performance evaluation of macro lens in digital close range photogrammetry (2009)

Recently, the documentation and visualization of various cultural heritages have been receiving attention, and a small Buddha such as less than 10 cm tall which was stored in the womb of Buddha is also included in cultural heritages. Zoom lenses are generally used to document these small objects and thus conserve the cultural heritage. However, there exist certain issues pertaining to the use of zoom lenses for such digital documentation. These issues include image sharpness and distortions that occur with changes in focal length setting, and in particular, the depth of field is issue from application standpoint such as documentation of the small cultural heritage. On the other hand, macro lenses can be used to capture sharp images of small objects from the view point of working distance, and its depth of field is related to the aperture of the camera. In order to evaluate the effectiveness of macro lenses in digital close range photogrammetry, macro lens and zoom lens were mounted on a digital single lens reflex camera (Canon EOS20D, 8.2 Mega pixels). This paper deals in a first part with comparative evaluations for both lenses with respect to their lens distortion, imaging mode, and calibration aspects. The results indicated that macro lenses were more suitable for digital close range photogrammetry. Calibration tests are performed to demonstrate the effectiveness and practicability of macro lens in close range photogrammetic applications.
Reciprocal Limbic-Cortical Function and Negative Mood: Converging PET Findings in Depression and Normal Sadness (1999)

Reciprocal changes involving subgenual cingulate and right prefrontal cortex occur with both transient and chronic changes in negative mood. The presence and maintenance of functional reciprocity between these regions with shifts in mood in either direction suggests that these regional interactions are obligatory and probably mediate the well-recognized relationships between mood and attention seen in both normal and pathological conditions. The bidirectional nature of this limbic-cortical reciprocity provides additional evidence of potential mechanisms mediating cognitive ("top-down"), pharmacological (mixed), and surgical ("bottom-up") treatments of mood disorders such as depression.
Functional Analysis of anArabidopsisTranscription Factor, DREB2A, Involved in Drought-Responsive Gene Expression (2006)

Transcription factors DREB1A/CBF3 and DREB2A specifically interact with cis-acting dehydration-responsive element/C-repeat (DRE/CRT) involved in cold and drought stress-responsive gene expression in Arabidopsis thaliana. Intact DREB2A expression does not activate downstream genes under normal growth conditions, suggesting that DREB2A requires posttranslational modification for activation, but the activation mechanism has not been clarified. DREB2A domain analysis using Arabidopsis protoplasts identified a transcriptional activation domain between residues 254 and 335, and deletion of a region between residues 136 and 165 transforms DREB2A to a constitutive active form. Overexpression of constitutive active DREB2A resulted in significant drought stress tolerance but only slight freezing tolerance in transgenic Arabidopsis plants. Microarray and RNA gel blot analyses revealed that DREB2A regulates expression of many water stress-inducible genes. However, some genes downstream of DREB2A are not downstream of DREB1A, which also recognizes DRE/CRT but functions in cold stress-responsive gene expression. Synthetic green fluorescent protein gave a strong signal in the nucleus under unstressed control conditions when fused to constitutive active DREB2A but only a weak signal when fused to full-length DREB2A. The region between DREB2A residues 136 and 165 plays a role in the stability of this protein in the nucleus, which is important for protein activation.
Nanoscale porosity in polymer films: fabrication and therapeutic applications (2010)

This review focuses on current developments in the field of nanostructured bulk polymers and their application in bioengineering and therapeutic sciences. In contrast to well-established nanoscale materials, such as nanoparticles and nanofibers, bulk nanostructured polymers combine nanoscale structure in a macroscopic construct, which enables unique application of these materials. Contemporary fabrication and processing techniques capable of producing nanoporous polymer films are reviewed. Focus is placed on techniques capable of sub-100 nm features since this range approaches the size scale of biological components, such as proteins and viruses. The attributes of these techniques are compared, with an emphasis on the characteristic advantages and limitations of each method. Finally, application of these materials to biofiltration, immunoisolation, and drug delivery are reviewed.
Detection of circulating tumour cells and nodal metastasis by reverse transcriptase-polymerase chain reaction technique. (1997)

This technique may be useful in the early diagnosis of primary or metastatic tumours, in assessing prognosis and in detecting residual disease after treatment.
Highly Selective Oligonucleotide‐Based Sensor for Mercury(II) in Aqueous Solutions (2004)

Mercury gauge: An oligodeoxyribonucleotide(ODN)-based sensor, which carries energy-donor and -acceptor moieties (fluorescein, F, and dabcyl, D, respectively), selectively detects HgII ions in aqueous solution. Upon binding HgII ions, the ODN takes on a hairpin structure (see picture); this results in a decrease in the fluorescence emission through enhanced FRET (fluorescence resonance energy transfer) from F to D. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2004/z54172_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
E3 Ubiquitin Ligase HOIP Attenuates Apoptotic Cell Death Induced by Cisplatin (2014)

The genotoxin cisplatin is commonly used in chemotherapy to treat solid tumors, yet our understanding of the mechanism underlying the drug response is limited. In a focused siRNA screen, using an siRNA library targeting genes involved in ubiquitin and ubiquitin-like signaling, we identified the E3 ubiquitin ligase HOIP as a key regulator of cisplatin-induced genotoxicity. HOIP forms, with SHARPIN and HOIL-1L, the linear ubiquitin assembly complex (LUBAC). We show that cells deficient in the HOIP ligase complex exhibit hypersensitivity to cisplatin. This is due to a dramatic increase in caspase-8/caspase-3-mediated apoptosis that is strictly dependent on ATM-, but not ATR-mediated DNA damage checkpoint activation. Moreover, basal and cisplatin-induced activity of the stress response kinase JNK is enhanced in HOIP-depleted cells and, conversely, JNK inhibition can increase cellular resistance to cisplatin and reverse the apoptotic hyperactivation in HOIP-depleted cells. Furthermore, we show that HOIP depletion sensitizes cancer cells, derived from carcinomas of various origins, through an enhanced apoptotic cell death response. We also provide evidence that ovarian cancer cells classified as cisplatin-resistant can regain sensitivity following HOIP downregulation. Cumulatively, our study identifies a HOIP-regulated antiapoptotic signaling pathway, and we envisage HOIP as a potential target for the development of combinatorial chemotherapies to potentiate the efficacy of platinum-based anticancer drugs.
Optimization of enzyme complexes for lignocellulose hydrolysis (2006)

The ability of a commercial Trichoderma reesei cellulase preparation (Celluclast 1.5L), to hydrolyze the cellulose and xylan components of pretreated corn stover (PCS) was significantly improved by supplementation with three types of crude commercial enzyme preparations nominally enriched in xylanase, pectinase, and beta-glucosidase activity. Although the well-documented relief of product inhibition by beta-glucosidase contributed to the observed improvement in cellulase performance, significant benefits could also be attributed to enzymes components that hydrolyze non-cellulosic polysaccharides. It is suggested that so-called "accessory" enzymes such as xylanase and pectinase stimulate cellulose hydrolysis by removing non-cellulosic polysaccharides that coat cellulose fibers. A high-throughput microassay, in combination with response surface methodology, enabled production of an optimally supplemented enzyme mixture. This mixture allowed for a approximately twofold reduction in the total protein required to reach glucan to glucose and xylan to xylose hydrolysis targets (99% and 88% conversion, respectively), thereby validating this approach towards enzyme improvement and process cost reduction for lignocellulose hydrolysis.