gebhart/patrepeval huggingface dataset:
Microbiological studies on drugs and their raw materials. IV. Sterilization of microbial contaminants in enzyme powder by gamma irradiation. (1978)

The decimal reduction dose of γ-ray on freeze-dried clean Escherichia coli cells and Bacillus subtilis spores was about 7×104 rad and it was about three times higher than that on E. coli cells suspened in saline. E. coli cells contained in Takadiastase or trypsin powder showed quite the same susceptibility to γ-irradiation as they were present cleanly. These enzyme activities were not impaired at all even at the dose of 2×105 rad. When these enzyme powders containing E. coli cells were stored under varied atmospheric relative humidity, the deleterious effect of γ-ray on bacterial cells was highly enhanced in those samples stored under more than 80.5% relative humidities. The additions of excipients, such as glucose and lactose, and of a protectant, L-cysteine, to the bacteria-containing dry enzyme powder did not show any sign of either enhancement or retardation of γ-ray action on enzyme and bacteria. Based on these observations, the utilizability of radiosterilization on biological medicaments is discussed.
Ion formation from charged droplets: Roles of geometry, energy, and time (1993)

The formation of ions from the charged droplets produced in the several spray ionization techniques is viewed as an activated rate process involving field-assisted desorption, in accord with the ideas first set forth by Iribame and Thomson. The novel features of the present treatment are particularly relevant to the unique ability of electrospray ionization to transform large molecules in solution to free ions in the gas phase, with extensive multiple charging. These new features stem mainly from the realization that the spacing of charges on a desorbed ion must relate to the spacing of charges on the surface of the droplet whence it came. The consequences of this "rule" can account for the existence of maxima and minima in the number of charges on the ions of a particular species as well as the nature of the distribution of ions among the intervening charge states. They also explain the dependence of charge state on the configuration in solution of the parent molecule of the desorbed ion. In addition, they provide insight into the sequence in time at which ions in the various charge states leave an evaporating droplet.
Human Seminal Vesicle-Specif ic Antigen is a Substrate for Prostate-Specific Antigen (or P-30)1 (1988)

Human seminal vesicle and prostatic fluids were obtained separately and reconstituted in vitro to test the hypothesis that proteolytic enzymes of prostatic origin would degrade seminal vesicle-specific antigen (SVSA). unable to degrade SVSA. These results demonstrate that SVSA is a substrate for PSA during human semen liquefaction.
Reactive hemophagocytic syndrome in adult systemic disease: Report of twenty‐six cases and literature review (2003)

When RHS occurs in the course of an active systemic disease (situation only reported in cases of systemic lupus or adult Still's disease), immunosuppressive therapy should be used. In contrast, when RHS is present concomitantly with an active infection, immunosuppressive therapy needs to be lowered and antibiotic therapy should be instituted.
Cardiovascular risk and the omega-3 index (2007)

A reliable risk factor for sudden cardiac death (SCD) for the general population remains to be defined. We propose the omega-3 index, defined as the combined percentage of eicosapentaenoic (EPA) and docosahexaenoic acids (DHA) in red blood cell membranes. It reflects the EPA + DHA status of a given individual. It can be determined by a standardised and reproducible laboratory procedure. Several lines of evidence support the omega-3 index as a risk factor for SCD: in epidemiological studies, a steep dependence of risk for SCD and the omega-3 index has been observed between 6.5% (risk 0.1) and 3.3% (risk 1.0). EPA + DHA are antiarrhythmic on the supraventricular and ventricular levels. Dietary EPA + DHA reduce the incidence of SCD. Cardiac societies recommend EPA + DHA for prevention of SCD. The omega-3 index can assess risk for SCD and monitor therapy with EPA + DHA. Moreover, it compares very favourably with other risk factors for SCD.
STEROL METHYLTRANSFERASE 1 Controls the Level of Cholesterol in Plants (2000)

The side chain in plant sterols can have either a methyl or ethyl addition at carbon 24 that is absent in cholesterol. The ethyl addition is the product of two sequential methyl additions. Arabidopsis contains three genes-sterol methyltransferase 1 (SMT1), SMT2, and SMT3-homologous to yeast ERG6, which is known to encode an S-adenosylmethionine-dependent C-24 SMT that catalyzes a single methyl addition. The SMT1 polypeptide is the most similar of these Arabidopsis homologs to yeast Erg6p. Moreover, expression of Arabidopsis SMT1 in erg6 restores SMT activity to the yeast mutant. The smt1 plants have pleiotropic defects: poor growth and fertility, sensitivity of the root to calcium, and a loss of proper embryo morphogenesis. smt1 has an altered sterol content: it accumulates cholesterol and has less C-24 alkylated sterols content. Escherichia coli extracts, obtained from a strain expressing the Arabidopsis SMT1 protein, can perform both the methyl and ethyl additions to appropriate sterol substrates, although with different kinetics. The fact that smt1 null mutants still produce alkylated sterols and that SMT1 can catalyze both alkylation steps shows that there is considerable overlap in the substrate specificity of enzymes in sterol biosynthesis. The availability of the SMT1 gene and mutant should permit the manipulation of phytosterol composition, which will help elucidate the role of sterols in animal nutrition.
Magnetic film inductors for radio frequency applications (1997)

Responding to the increasing demand for miniaturization and circuit integration of rf inductors we analyze various aspects of designing planar inductive circuits based on magnetic films as well as demonstrate substantially enhanced inductance characteristics for some prototype structures.
DNA methylation biomarkers in cancer: progress towards clinical implementation (2012)

Altered DNA methylation is ubiquitous in human cancers and specific methylation changes are often correlated with clinical features. DNA methylation biomarkers, which use those specific methylation changes, provide a range of opportunities for early detection, diagnosis, prognosis, therapeutic stratification and post-therapeutic monitoring. Here we review current approaches to developing and applying DNA methylation biomarkers in cancer therapy. We discuss the obstacles that have so far limited the routine use of DNA methylation biomarkers in clinical settings and describe ways in which these obstacles can be overcome. Finally, we summarize the current state of clinical implementation for some of the most widely studied and well-validated DNA methylation biomarkers, including SEPT9, VIM, SHOX2, PITX2 and MGMT.
Conformational analysis of the interdomain linker of the central homology region of chloroplast initiation factor IF3 supports a structural model of two compact domains connected by a flexible tether (1998)

A peptide corresponding to the interdomain linker of chloroplast IF3 has been synthesized and its structure studied by NMR and CD as a function of temperature and pH. At low temperature and neutral pH the apparent helical content is 25%. pH and ionic strength dependent CD studies demonstrate that sidechain-sidechain interactions stabilize the structure observed at low temperature. The helicity decreases with temperature and above 25 degrees C the peptide is less than 15% helical. These results indicate that the peptide has little intrinsic tendency to form helical structure at physiologically relevant temperatures and strongly suggests that the linker region is flexible in intact chloroplast IF3.
Expression profiles of ErbB family receptors and prognosis in primary transitional cell carcinoma of the urinary bladder. (2001)

In vitro experiments have demonstrated that epidermal growth factor (EGF)-related peptides activate distinct subsets of ErbB receptors and differ in their biological activities. The implications of cross-talk among ErbB family receptors in human cancer, however, remain to be clarified. This cohort study was performed to examine the expression patterns of ErbB receptors by immunohistochemistry in primary human bladder cancer (n = 245) and compared with conventional biological indicators for their prognostic significance. Expression of individual EGF receptor (EGFR) and ErbB2, ErbB3, or ErbB4 receptors was detected in 72.2, 44.5, 56.3, and 29.8% of bladder cancer cases, respectively. Expression of two of the receptors varied from 14.7 to 42.4%, of three of the receptors between 11.0 and 22.0%, and of all four of the ErbB receptors by 8.6%. Important indicators in association with patient survival were tumor staging (P = 0.017), ErbB2 (P = 0.018), EGFR-ErbB2 (P = 0.023), and ErbB2-ErbB3 (P = 0.042). In the subset of grade-2 tumors, EGFR-ErbB2-ErbB3 and EGFR-ErbB2 predicted the development of second recurrence (P = 0.026 and 0.039, respectively), and ErbB2-ErbB3 tended to correlate with patient survival (P = 0.09). The results indicate that a combination of EGFR, ErbB2, and ErbB3 expression profile may be a better prognostic indicator than any family member alone. Given that ErbB2 is the preferred coexpression partner of ErbB family members, expression of other ErbB receptors may significantly affect the prognostic implication of ErbB2 for bladder cancer patients.
Antifungal Activities of Major Tea Leaf Volatile Constituents toward Colletorichum camelliae Massea (2006)

A crude glycosidic fraction was prepared from fresh tea leaves and treated with the crude tea enzyme, fractions of cis-3-hexenol, linalool oxide I (cis-furanoid), linalool oxide II (trans-furanoid), linalool, methyl salicylate, geraniol, benzyl alcohol, and 2-phenylethanol were monitored to be the major aglycone moieties by analyzing the released volatiles. The amount of the released aglycone moieties is 5.8 times higher than those in free form. For investigation of the functions of the glycosidically bound form aroma constituents in tea leaves, their antifungal activities were determined by antifungal assay. Geraniol, linalool, methyl salicylate, benzyl alcohol, and 2-phenylethanol exhibited significant antifungal activities toward Colletorichum camelliae Massea, although cis-3-hexenol and linalool oxides showed weaker activities by comparison. Among them, geraniol was shown to be the most potential antifungal substance with a MIC value of 440 microg/mL. The crude glycosidic fraction prepared from tea leaves also exhibited significant antifungal activities in a wide range of concentrations from 2 to 25 mg/mL in a PDA medium. It was deduced that the glycosidically bound volatiles are formed and stored in the intact tissue of tea leaf and hydrolyzed by the actions of both the endogenous and the exogenous glycosidases to release volatiles as antifungal substances when exposed to Colletorichum camelliae Massea. The results suggested that the higher content of the bound form geraniol in tea leaves of var. sinensis might be responsible for their stronger antipathogen properties toward tea leaf blight, as opposed to those of var. assamica.
Apelin and energy metabolism (2015)

A wide range of adipokines identified over the past years has allowed considering the white adipose tissue as a secretory organ closely integrated into overall physiological and metabolic control. Apelin, a ubiquitously expressed peptide was known to exert different physiological effects mainly on the cardiovascular system and the regulation of fluid homeostasis prior to its characterization as an adipokine. This has broadened its range of action and apelin now appears clearly as a new player in energy metabolism in addition to leptin and adiponectin. Apelin has been shown to act on glucose and lipid metabolism but also to modulate insulin secretion. Moreover, different studies in both animals and humans have shown that plasma apelin concentrations are usually increased during obesity and type 2 diabetes. This mini-review will focus on the various systemic apelin effects on energy metabolism by addressing its mechanisms of action. The advances concerning the role of apelin in metabolic diseases in relation with the recent reports on apelin concentrations in obese and/or diabetic subjects will also be discussed.
Individual Struggles for Selfhood: Conflicts and Tensions of Transforming Self Within Cultural Worlds (2005)

Personal histories of selected New Zealand (NZ) farm women (including the researcher-as-subject) are engaged to explore the relations between the social settings and practices in which individuals participate, and their subjectivities. These relations are held to shape how social and individual factors are transformed through tensions and conflicts as work-life roles and identities are played out. From this, a changing sense of self in relation to the social world and human agency is examined in terms of the formation, continuity and transformation of these farm wives' identities. New norms or expectations are seen as constructed through resolving conflicts and tensions and experiencing considerable negotiation of the self. While social position within the workplace and culture is fluid and shifting, a sense of self is embedded in the cultural and social experiences created through participation within the community and practice. Within all this, a search for coherence and authenticity (Fenwick, 2000) may be guiding individuals' agentic efforts through tension, conflict and constant questioning. Struggles for selfhood or a retreat to safety and survival mode may temporarily, and somewhat falsely, bury a feeling of alienation, which remains suppurating below the surface (Fenwick, 1999). A range of women's voices are sought to understand the process by which cultural meanings are known, challenged and changed.
High-mobility thin-film transistor with amorphous InGaZnO4 channel fabricated by room temperature rf-magnetron sputtering (2006)

Thin-film transistors (TFTs) were fabricated using amorphous indium gallium zinc oxide (a-IGZO) channels by rf-magnetron sputtering at room temperature. The conductivity of the a-IGZO films was controlled from ∼10−3to10−6Scm−1 by varying the mixing ratio of sputtering gases, O2∕(O2+Ar), from ∼3.1% to 3.7%. The top-gate-type TFTs operated in n-type enhancement mode with a field-effect mobility of 12cm2V−1s−1, an on-off current ratio of ∼108, and a subthreshold gate voltage swing of 0.2Vdecade−1. It is demonstrated that a-IGZO is an appropriate semiconductor material to produce high-mobility TFTs at low temperatures applicable to flexible substrates by a production-compatible means.
Efficacy, safety, and pharmacokinetics of multiple administration of infliximab in Behçet's disease with refractory uveoretinitis. (2004)

Administration of infliximab in patients with BD with refractory uveoretinitis suppressed the frequency of ocular attacks, and multiple administration was well tolerated, suggesting that infliximab is effective for this condition.
231. Syntheses of certain thiazolopyrimidines (4 : 5 : 6 : 9-tetrahydro-1-thia-7 : 9-diazaindanes) (1950)

P. J. Heald and T. K. Walker, J. Chem. Soc., 1950, 1127 DOI: 10.1039/JR9500001127
A Cell Classifier for RRAM Process Development (2015)

Devices that exhibit resistive switching are promising components for future nanoelectronics with applications ranging from emerging memory to neuromorphic computing and biosensors. In this brief, we present an algorithm for identifying switchable devices, i.e., devices that can be programmed in distinct resistive states and that change their state predictably and repeatedly in response to input stimuli. The method is based on extrapolating the statistical significance of difference in between two distinct resistive states as measured from devices subjected to standardized bias protocols. The test routine is applied on distinct elements of 32×32 resistive-random-access-memory (RRAM) crossbar arrays and yields a measure of device switchability in the form of a statistical significance p-value. Ranking devices by p-value shows that switchable devices are typically found in the bottom 10% and are therefore easily distinguishable from nonfunctional devices. Implementation of this algorithm dramatically cuts RRAM testing time by granting fast access to the best devices in each array, as well as yield metrics.
Crystal Structure of the Principal Neutralization Site of HIV-1 (1994)

The crystal structure of a complex between a 24-amino acid peptide from the third variable (V3) loop of human immunodeficiency virus-type 1 (HIV-1) gp 120 and the Fab fragment of a broadly neutralizing antibody (59.1) was determined to 3 angstrom resolution. The tip of the V3 loop containing the Gly-Pro-Gly-Arg-Ala-Phe sequence adopts a double-turn conformation, which may be the basis of its conservation in many HIV-1 isolates. A complete map of the HIV-1 principal neutralizing determinant was constructed by stitching together structures of V3 loop peptides bound to 59.1 and to an isolate-specific (MN) neutralizing antibody (50.1). Structural conservation of the overlapping epitopes suggests that this biologically relevant conformation could be of use in the design of synthetic vaccines and drugs to inhibit HIV-1 entry and virus-related cellular fusion.
Molecular mechanisms of drug resistance involving ribonucleotide reductase: hydroxyurea resistance in a series of clonally related mouse cell lines selected in the presence of increasing drug concentrations. (1988)

Mammalian ribonucleotide reductase is a highly regulated, rate-limiting activity responsible for converting ribonucleoside diphosphates to the deoxyribonucleotide precursors of DNA. The enzyme consists of two nonidentical proteins often called M1 and M2, both of which are required for activity. Hydroxyurea is an antitumor agent which inhibits ribonucleotide reductase by interacting with the M2 component specifically at a unique tyrosyl free radical. To obtain further information about drug resistance mechanisms, we have used M1 and M2 complementary DNAs and monoclonal antibodies to investigate the properties of a series of clonally related drug-resistant mouse cell lines, selected by a step-wise procedure for increasing levels of resistance to the cytotoxic effects of hydroxyurea. Several interesting mechanisms have been identified. Each successive drug selection step leading to the isolation of highly resistant cells was accompanied by stable elevations in cellular resistance and ribonucleotide reductase activities. The changes that occurred at each step involved the M2 component. A very early event, occurring at the first step in the selection process, was the amplification of the M2 gene accompanied by an increase in M2 messenger RNA. Although cellular resistance and M2 protein levels increased significantly during drug selection, only a modest change in M2 gene copy number was observed after the initial selection step. Analysis of wild type, moderately resistant, and highly resistant cells indicated that, in addition to M2 gene amplification, posttranscriptional modification also occurred during drug selection. This second mechanism was not due to alterations in protein M2 half-life, but involved an increase in translational efficiency. By increasing the rate of M2 synthesis, without altering degradation rates, resistant cells were able to accumulate high levels of this key regulatory protein. Cells selected for the ability to proliferate in concentrations of drug as high as 4 mM exhibited changes that involved M2, without detectable changes to M1. These results provide further evidence that M1 and M2 levels are controlled by different mechanisms in mammalian cells. Eventually, however, cells required an elevation in the M1 protein, as well as the M2 protein, to survive in a hydroxyurea concentration of 5 mM. These results illustrate the complexity of the drug-resistant phenotype and provide further information about the molecular processes that lead to the development of cells resistant to low, intermediate, and high concentrations of hydroxyurea.
An Overview of Ensembl (2004)

Ensembl (http://www.ensembl.org/) is a bioinformatics project to organize biological information around the sequences of large genomes. It is a comprehensive source of stable automatic annotation of individual genomes, and of the synteny and orthology relationships between them. It is also a framework for integration of any biological data that can be mapped onto features derived from the genomic sequence. Ensembl is available as an interactive Web site, a set of flat files, and as a complete, portable open source software system for handling genomes. All data are provided without restriction, and code is freely available. Ensembl's aims are to continue to "widen" this biological integration to include other model organisms relevant to understanding human biology as they become available; to "deepen" this integration to provide an ever more seamless linkage between equivalent components in different species; and to provide further classification of functional elements in the genome that have been previously elusive.
The Tocolytic Effect of Catecholamines in the Gravid Rat Uterus (1998)

Maternal catecholamines increase in labor, but epinephrine decreases dramatically after regional analgesia. In this study, we found that norepinephrine and epinephrine together decrease uterine contractile activity and that decreased epinephrine causes significantly increased uterine activity.
A Serologic Marker of Paraneoplastic Limbic and Brain-Stem Encephalitis in Patients with Testicular Cancer (1999)

The serum of patients with subacute limbic and brain-stem dysfunction and testicular cancer contains antibodies against a protein found in normal brain and in testicular tumors. Detection of these antibodies supports the paraneoplastic origin of the neurologic disorder and could be of diagnostic importance.
Two Victorian Women Loved by Randolph Henry Ash (1995)

P(論文)
Comparative kinetic studies on the synthesis of quinoxalinone derivatives and pyrido[2,3‐b]pyrazinone derivatives by the hinsberg reaction (1992)

Abstract Kinetic studies on the anelation of quinoxalinone derivatives 3a‐c and pyrido[2,3‐ b ]pyrazinone derivatives 5a‐c and 6a‐c synthesized by the Hinsberg reaction is reported. o ‐Phenylenediamine or 2,3‐diaminopyridine were treated with bifunctional carbonyl compounds such as glyoxylic, pyruvic and benzoylformic acids under different experimental conditions. When pyridopyrazine derivatives were synthesized both position isomers were achieved applying regioselective reactions. Mixture were avoided by looking for special experimental conditions that led unambiguously to only one of the components of the classic “Hinsberg mixture”. Quinoxalinone derivatives 3a‐c were obtained at room temperature in good yields (&gt;90%) using anhydrous methanol or ethanol as solvents. On the other hand, only pyrido[2,3‐ b ]pyrazin‐3(4 H )‐one ( 5a ) was regioselectively attained in aqueous buffer of p H 7 while 3‐methylpyridopyrazinone derivatives were regioselectively separated using anhydrous methanol for one isomer, 5b , and anhydrous chloroform for the other isomer, 6b , at room temperature. Yields were higher than 80%. Reactions with benzoylformic acid did not give good yields and only 2‐phenylpyrido[2,3‐ b ]pyrazin‐3(4 H )‐one ( 5c ) could be obtained using anhydrous chloroform (yield &lt;30%) as the solvent. Steric hindrance exerted by the phenyl group of the benzoylformic acid is supposed to be responsible of our difficulties to obtain 2‐phenylpyrido[2,3‐ b ]pyrazin‐3(4 H )‐one ( 5c ) in good yields applying this technique. The other isomer, 3‐phenyl[2,3‐b]pyrazin‐2(1 H )‐one ( 6c ) was always formed together with the former isomer and could not be isolated from the mixture, when other solvents than chloroform were used as the reaction media.