gebhart/patrepeval huggingface dataset:
Closure of left ventricle perforation with the use of muscular VSD occluder (2005)

Growing experience in interventional cardiology leads to the use of large diameter of vascular equipment. In some instances, the so-called hybrid procedures are performed. After performing the interventional procedure, the opening in ventricular wall is closed surgically. Our intention was to check if the MVSDO can be used to close the perforation in the heart after the interventional cardiology procedure performed through the left ventricular (LV) free wall. In three pigs under general anesthesia, the heart was exposed through a small substernal incision. The LV was punctured and an 18F sheath was introduced into the LV. A 14mm MVSDO was inserted through the 10F Delivery System. Using both the echocardiographic and angiographic guidance, the MVSDO was placed on the LV wall to close the opening in the LV. Time and volume of bleeding was recorded. In all cases the occluder was successfully placed closing the opening, bleeding observed after deployment of occluder lasted for approximately 2min. We think MVSD occluder can be used to close the LV free wall perforation after hybrid interventional cardiac procedure. Early bleeding through MVSDO might be resolved by the manufacturing of new occluder with better sealing properties
Pilot Plant Production of an Edible Alfalfa Protein Concentrate (1981)

ABSTRACT This paper reports the development of a pilot plant scale wet fractionation process to obtain an edible leaf protein concentrate from alfalfa juice by the fractionated recovery of the chloroplastic and cytoplasmatic proteins. The green pigmented fraction is recovered at room temperature by employing an organic polyelectrolyte, while the edible white proteins are coagulated by acidification to pH 4.0. The pilot plant and working conditions are described together with the chemical composition of the final products. Some functional properties of cytoplasmatic protein concentrate are also reported. Since the whole process takes place at room temperature the cytoplasmatic protein concentrate shows good functional properties especially with regard to its nitrogen solubility.
A simplified tree bait for the mountain pine beetle (1993)

Seven, split-block experiments throughout British Columbia in 1989 tested the efficacy of binary tree baits containing the pheromones trans-verbenol and exo-brevicomin or ternary baits with the addition of the host tree kairomone myrcene for containing and concentrating infestations of the mountain pine beetle, Dendroctonusponderosae Hopkins, in stands of lodgepole pine, Pinuscontorta var. latifolia Engelm. Attack densities on baited trees, attack frequencies of baited trees and trees within 10 m of the baited trees, and the ratios of newly attacked, green, trees to previously attacked, red, trees were generally statistically equal between sub-blocks containing binary or ternary baits. Where statistically significant differences occurred for one or more of the above criteria in one experiment, they were generally offset by statistically significant differences in the opposite direction in another experiment. Two individual-tree experiments in 1990 that supported the equality of binary and ternary baits indicated that raising the release rate of trans-verbenol in binary baits tended to reduce their efficacy (possibly because of contamination with the antiaggregation pheromone verbenone) and showed that increasing the release rate of exo-brevicomin tended to counteract this effect. When attack frequencies were subdivided by diameter class of available trees attacked, all baits were effective in inducing attack on available trees &lt;30.0 cm diameter at breast height (1.3 m), but attack on baited and control trees ≥30 cm diameter at breast height was equal. Provided that the trans-verbenol in binary baits does not contain or autoxidize to verbenone, myrcene can be deleted from operational tree baits.
Coactivation of Rac1 and Cdc42 at Lamellipodia and Membrane Ruffles Induced by Epidermal Growth Factor (2003)

A major function of Rho-family GTPases is to regulate the organization of the actin cytoskeleton; filopodia, lamellipodia, and stress fiber are regarded as typical phenotypes of the activated Cdc42, Rac, and Rho, respectively. Using probes based on fluorescent resonance energy transfer, we report on the spatiotemporal regulation of Rac1 and Cdc42 at lamellipodia and membrane ruffles. In epidermal growth factor (EGF)-stimulated Cos1 and A431 cells, both Rac1 and Cdc42 were activated diffusely at the plasma membrane, followed by lamellipodial protrusion and membrane ruffling. Although Rac1 activity subsided rapidly, Cdc42 activity was sustained at lamellipodia. A critical role of Cdc42 in these EGF-induced morphological changes was demonstrated as follows. First, phorbol 12-myristate 13-acetate, which activated Rac1 but not Cdc42, could not induce full-grown lamellipodia in Cos1 cells. Second, a GTPase-activating protein for Cdc42, KIAA1204/CdGAP, inhibited lamellipodial protrusion and membrane ruffling without interfering with Rac1 activation. Third, expression of the Cdc42-binding domain of N-WASP inhibited the EGF-induced morphological changes. Therefore, Rac1 and Cdc42 seem to synergistically induce lamellipodia and membrane ruffles in EGF-stimulated Cos1 cells and A431 cells.
p62 Association with RNA Is Regulated by Tyrosine Phosphorylation (1995)

The ras-GAP associated protein, p62, is a major tyrosine phosphoprotein in transformed and growth factor treated cells. Although its exact function is not known, it can bind directly to src-family tyrosine kinases and has been implicated as a linker protein bridging activated src family tyrosine kinases with downstream effectors. One novel feature of p62, revealed by its predicted amino acid sequence, is the presence of an RNA-binding region, the KH domain. As p62 becomes tyrosine phosphorylated when src-kinases become activated, we compared the RNA binding ability of p62 in both its phosphorylated and unphosphorylated state. The ability of p62 to bind RNA was severely impaired when p62 was tyrosine phosphorylated. This suggests that the ability of p62 to bind RNA is regulated by tyrosine phosphorylation and implicates the regulation of RNA as a component of tyrosine kinase signaling pathways.
Use of anticonvulsants for treatment of neuropathic pain (2002)

Emerging evidence from animal models of neuropathic pain suggests that many pathophysiologic and biochemical changes occur in the peripheral and central nervous system. Similarities between the pathophysiologic phenomena observed in some epilepsy models and in neuropathic pain models justify the use of anticonvulsants in the symptomatic management of neuropathic pain. Positive results from laboratory and clinical trials further support such use. Carbamazepine was the first of this class of drugs to be studied in clinical trials and has been longest in use for treatment of neuropathic pain. Clinical trial data support its use in treating trigeminal neuralgia, but data for treatment of painful diabetic neuropathy are less convincing. Use of newer anticonvulsants has marked a new era in the treatment of neuropathic pain. Gabapentin has demonstrated efficacy, specifically in painful diabetic neuropathy and postherpetic neuralgia. Lamotrigine has been reported to be effective in relieving pain from trigeminal neuralgia refractory to other treatments, HIV neuropathy, and central post-stroke pain. Results from clinical trials of phenytoin are equivocal. Zonisamide's mechanisms of action suggest that it would be effective in controlling neuropathic pain symptoms. Other anticonvulsants, including lorazepam, valproate, topiramate, and tiagabine, have also been under investigation. Anecdotal experience provides support for studies with oxcarbazepine and levetiracetam for treating neuropathic pain. Evidence supporting the efficacy of anticonvulsants in treatment of such pain is evolving. Additional clinical trials should provide information that will better define their role in neuropathic pain.
Pantetheine‐Free Mutants of the Yeast Fatty‐Acid‐Synthetase Complex (1973)

Among 52 Saccharomyces cerevisiae fatty acid synthetase ( fas )mutants screened for their ability to incorporate 14 C‐labeled pantothenic acid into the fatty acid synthetase multienzyme complex, especially those of fas ‐complementation group VII had lost this ability. The purified fatty acid synthetase complexes of all the 19 fas ‐mutants available from this group were shown to be free of 14 C‐labeled pantothenic acid. The amount of pantothenate incorporated into the enzymes of several other fas ‐mutants of the non‐pleiotropic complementation groups II, V, VI and VIII, however, was similar to that observed with wild‐type fatty acid synthetase. The purified pantothenate‐free fatty acid synthetases of five different group VII fas ‐mutants have been tested for the seven known component enzymes of the complex. In all mutants, only the β‐ketoacyl synthetase was inactive whereas in vitro all the other partial activities were unimpaired. By sodium dodecylsulfate‐polyacrylamide‐gel electrophoresis, no differences could be observed between the protein structure of the pantothenate‐free mutant fatty acid synthetase and that of the wild‐type comp ex. Both were separated into three different components A, B and C with molecular weights of 185000, 180000 and 177000, respectively. However, some fas ‐mutants consist only of the components A and B, others only of B and C. The study of various [ 14 C]‐pantothenate‐labeled mutant fatty acid synthetases suggests that component C originates from A presumably by limited proteolysis. In the undegraded complex AB, [ 14 C]pantothenate is only associated with the component A. Since in one of the mutants studied A is completely converted to C, it is concluded that A is one distinct component rather than a group of components with identical molecular weights. It is tentatively suggested that the gene product of fas 2, one of the two known fatty‐acid‐synthetase gene clusters in yeast, is only one, single and multifunctional polypeptide chain. Therefore, it appears that in yeast, the acyl carrier protein is not an individual protein component of the fatty acid synthetase complex, but only a distinct region of the multifunctional polypeptide chain encoded by fas 2.
Distribution and Phylogenetic Significance of 27-Hydroxy-Octacosanoic Acid in Lipopolysaccharides from Bacteria: Belonging to the Alpha-2 Subgroup of Proteobacteria (1991)

Lipopolysaccharides (LPSs) from a number of bacteria belonging to the alpha-2 subgroup of the class Proteobacteria were screened for the presence of 27-hydroxy-octacosanoic acid (27-OH-28:0). With few exceptions, most of the bacteria contained 27-OH-28:0 in their lipid A fractions. In addition, some of the bacteria contained other n-2-hydroxylated long-chain fatty acids hitherto not reported. The distribution of 27-OH-28:0 was restricted to the alpha-2 subgroup. LPSs from members of the other subgroups (the beta and gamma subgroups), including some well-characterized enterobacterial LPSs, were devoid of 27-OH-28:0. Our results indicate that the presence of n-2-hydroxylated long-chain fatty acids in LPSs might be used as a chemophylogenetical marker.
Regnase-1 Maintains Iron Homeostasis via the Degradation of Transferrin Receptor 1 and Prolyl-Hydroxylase-Domain-Containing Protein 3 mRNAs (2017)

Iron metabolism is regulated by transcriptional and post-transcriptional mechanisms. The mRNA of the iron-controlling gene, transferrin receptor 1 (TfR1), has long been believed to be negatively regulated by a yet-unidentified endonuclease. Here, we show that the endonuclease Regnase-1 is critical for the degradation of mRNAs involved in iron metabolism in vivo. First, we demonstrate that Regnase-1 promotes TfR1 mRNA decay. Next, we show that Regnase-1<sup>-/-</sup> mice suffer from severe iron deficiency anemia, although hepcidin expression is downregulated. The iron deficiency anemia is induced by a defect in duodenal iron uptake. We reveal that duodenal Regnase-1 controls the expression of PHD3, which impairs duodenal iron uptake via HIF2α suppression. Finally, we show that Regnase-1 is a HIF2α-inducible gene and thus provides a positive feedback loop for HIF2α activation via PHD3. Collectively, these results demonstrate that Regnase-1-mediated regulation of iron-related transcripts is essential for the maintenance of iron homeostasis.
Hydrothermal syntheses and structures of three novel coordination polymers assembled from 1,2,3-triazolate ligands (2009)

Three new coordination polymers with 1,2,3-triazolate ligands, [Cd3(ta)3Cl3]n (1), {[Cd3(ta)6]·6H2O}n (2) and [Cu6(ta)3I3]n (3) (Hta = 1,2,3-triazole), have been hydrothermally synthesized and structurally characterized. In complex 1, the Cd2+ and Cl− ions form a 3D [(Cd3Cl3)3+]n inorganic framework with (82.12)(4.8.10) topology while the Cd2+ ions and ta− ligands give a {[Cd3(ta)3]3+}n double helical chain. The two moieties are fused with each other by sharing “hinge” Cd2+ ions leading to a 3D organic–inorganic hybrid structure. The 3D porous metal–organic framework of complex 2 shows the topology of the diamond net with the Cd-centered CdCd4 tetrahedron units as the connecting nodes. The desolvated structure of 2 has 48% solvent accessible space of the total volume. Complex 3 is an undulating 1D organic–inorganic hybrid complex consisting of interesting discrete five-capped [Cu6I5]− triangular prism clusters and arched metallacycle [Cu6(ta)6I]+ moieties.
Ethylene in Soil Biology (1976)

Beneficial microbes in the microbiome of plant roots improve plant health. Induced systemic resistance (ISR) emerged as an important mechanism by which selected plant growth–promoting bacteria and fungi in the rhizosphere prime the whole plant body for ...Read More
679. Tocopherols. Part IV. Synthesis of 5-methyltocol (1959)

D. McHale, P. Mamalis, S. Marcinkiewicz and J. Green, J. Chem. Soc., 1959, 3358 DOI: 10.1039/JR9590003358
Radiation Protection and Radiation Recovery with Essential Metalloelement Chelates (1995)

Understanding essential metalloelement metabolism and its role in tissue maintenance and function, as well as the roles of essential metalloelement-dependent enzymes in responding to injury, offer a new approach to decreasing and/or treating radiation injury. This review presents the roles of some essential metalloelement-dependent enzymes in tissue maintenance and function, and their responses to radiation injury in accounting for radiation protection and recovery effects observed for nontoxic doses of essential metalloelement compounds. Effects of biochemicals including water undergoing bond radiolysis and the effects of free radicals derived from diatomic oxygen account for the acute and chronic aspects of radiation injury. Recognized biochemical roles of essential metalloelement-dependent enzymes and the observed pharmacological effects of small-molecular mass chelates predict the therapeutic usefulness of essential metalloelement complexes in decreasing and/or treatment of radiation injury. Copper chelates have radiation protection and radiation recovery activities and cause rapid recovery of immunocompetency and recovery from radiation-induced histopathology. Mice treated with Cu(II)2(3,5-diisopropylsalicylate)4[Cu (II)2(3,5-DIPS)4] had increased survival and corresponding increases in numbers of myeloid and multipotential progenitor cells early after irradiation and earlier recovery of immune reactivity. Examination of radiation-induced histopathology in spleen, bone marrow, thymus, and small intestine also revealed Cu(II)2(3,5-DIPS)4-mediated rapid recovery of radiation-induced histopathology. Most recently, Fe, Mn, and Zn complexes have also been found to prevent death in lethally irradiated mice. These pharmacological effects of essential metalloelement chelates can be understood as due to facilitation of de novo synthesis of essential metalloelement-dependent enzymes which have roles in preventing the accumulation of pathological concentrations of oxygen radicals or repairing biochemical damage caused by radiation-induced bond homolysis. Essential metalloelement chelates offer a physiological approach to prevention and/or treatment of radiation injury.
Parallel-split shadow maps for large-scale virtual environments (2006)

Shadowing effects dramatically enhance the realism of virtual environments by providing useful visual cues. Shadow mapping is an efficient algorithm for real-time shadow rendering, which is extensively adopted in real-time applications by its generality and efficiency. However, shadow mapping usually suffers from the inherent aliasing errors due to the image-based nature. In this paper, we present the Parallel-Split Shadow Maps (PSSMs) scheme, which splits the view frustum into different parts by using the planes parallel to the view plane and then generates multiple smaller shadow maps for the split parts. A fast and robust split strategy based on the analysis of shadow map aliasing has been proposed, which produces a moderate aliasing distribution over the whole depth range. By applying the geometry approximation procedure to each of the split parts instead of the whole scene, the tighter bounding shapes of visible objects enhance the utilization of the shadow map resolution. Hardware-acceleration is used to remove the extra rendering passes when synthesizing the scene-shadows. Our approach is intuitive to implement without using complex data structures, with real-time performance for dynamic and large-scale virtual environments.
Degradation Products Formed from Glucosamine in Water (1998)

An aqueous solution of glucosamine hydrochloride was heated to 150 °C for 5 min under different pH conditions. The reaction product mixture obtained was analyzed by GC/MS. It was found that the major products formed were furfurals, especially at pH = 4 and 7. At pH = 8.5, additional flavor components were generated, including pyrazines, 3-hydroxypyridines, pyrrole-2-carboxaldehyde, furans, acetol, and several other compounds. Of the components identified, it is worthwhile to note the formation of pyrazine and methylpyrazine as major components at pH = 8.5. It is proposed that a retro-aldol condensation plays an important role in the formation of the intermediates, α-aminoacetaldehyde (I) and α-amino propanal (II). As a result, self-condensation of I generates pyrazine and combination of I and II generates methylpyrazine. In addition, it is also interesting to note the formation of 3-hydroxypyridines and pyrrole-2-carboxaldehyde. It is suggested that both groups of compounds are derived from furfurals. As the ammonia is liberated from glucosamine, it initiates the ring-opening of furfurals to form 5-amino-2-keto-3-pentenals. Intramolecular condensations of these intermediates between the amino group and the carbonyl groups lead to the formation of 3-hydroxypyridines and pyrrole-2-carboxaldehyde. Keywords: Glucosamine; retro-aldol condensation; α-amino acetaldehyde; α-amino propanal; GC/MS
Assessment of Cancer-Related Neuropathy and Neuropathic Pain (2010)

Cancer-related neuropathic pain syndromes are common and serious complications of a patient's primary malignancy or its treatment, whether by surgery, radiation, or chemotherapy. They may compromise the patient's quality of life as well as their ability to receive effective treatment. In many patients, there may be more than one coexistent neuropathic pain syndrome, posing a diagnostic dilemma that, if unresolved, may result in the institution of therapies that are of limited scope or not targeted at the primary underlying pathophysiology. There is no single adequate diagnostic method that has been established to reliably diagnose or follow patients with cancer-related neuropathic pain syndromes. Clinical assessment of cancer-related neuropathic pain poses some important challenges diagnostically as well as in defining a clear and reliable endpoint assessment in controlled clinical trials. Many different approaches have been applied to the development of assessment or diagnostic tools. Careful review of these methods has been helpful in developing a clearer vision for the future design and refinement of more reliable tools, and more importantly, validation of the clinical utility as well as the reliability of such tools when employed as endpoints in clinical trials focused on prevention, mitigation, or treatment of cancer neuropathic pain.
Fabrication of large arrays of cortical electrodes for use in man (1977)

Abstract Techniques are described for fabrication of electrode arrays for stimulation and/or recording from a human cerebral cortex. These arrays may be neurosurgically inserted (with or without a Penrose drain) in the course of a conventional craniotomy or craniectomy, and are easily removed without reopening the ineision. Termination of the connecting wires in a special percutaneous pedestal has also permitted chronic implantation. The contacts and connecting wires are made of pure platinum embedded in a Teflon ribbon‐cable matrix. The choice of materials combines biological acceptability, electrochemical stability, and integrity of the insulation, but imposes special fabrication procedures.
Immunomodulatory oligonucleotides containing a cytosine-phosphate-2′-deoxy-7-deazaguanosine motif as potent Toll-like receptor 9 agonists (2005)

Bacterial DNA and synthetic oligomers containing CpG dinucleotides activate the immune system through Toll-like receptor (TLR) 9. Here, we compare the immunostimulatory activity of three immunomers with different nucleotide sequences containing a synthetic cytosine-phosphate-2'-deoxy-7-deazaguanosine dinucleotide (CpR), called immunomodulatory oligonucleotides (IMOs), in mouse, human, and monkey systems. IMOs induced IL-12 and IFN-gamma secretion more than a control non-CpG IMO in mice. All three IMOs activated HEK293 cells expressing TLR9 but not TLR3, -7, or -8. IMOs induced human B-cell proliferation and enhanced expression of CD86 and CD69 surface markers on B cells. The three IMOs induced CD86 expression on human plasmacytoid dendritic cells, but only IMOs that contained a 5'-terminal TCR nucleotide sequence induced IFN-alpha secretion. A sequence that forms a duplex structure also was required for IFN-alpha induction in human peripheral blood mononuclear cell cultures. IMOs induced chemokine and cytokine gene expression in human peripheral blood mononuclear cells. In monkeys, all three IMOs induced transient changes in peripheral blood leukocytes and lymphocytes and activated B and T lymphocytes. All three IMOs induced IFN-alpha in vivo in monkeys; the IMO sequence that forms a stable secondary structure induced the highest levels of IFN-alpha. These studies are, to our knowledge, the first comprehensive studies to compare the activity of IMOs containing synthetic stimulatory CpR dinucleotides in mouse, monkey, and human systems. These results suggest that IMOs induce strong and rapid immunostimulation and that the CpR dinucleotide is recognized by TLR9, leading to immune-cell activation and cytokine secretion in vitro and in vivo.
Fast DNA Sequencing via Transverse Electronic Transport (2006)

A rapid and low-cost method to sequence DNA would usher in a revolution in medicine. We propose and theoretically show the feasibility of a protocol for sequencing based on the distributions of transverse electrical currents of single-stranded DNA while it translocates through a nanopore. Our estimates, based on the statistics of these distributions, reveal that sequencing of an entire human genome could be done with very high accuracy in a matter of hours without parallelization, that is, orders of magnitude faster than present techniques. The practical implementation of our approach would represent a substantial advancement in our ability to study, predict, and cure diseases from the perspective of the genetic makeup of each individual.
Cardiac and systemic rejuvenation after cardiosphere-derived cell therapy in senescent rats (2017)

Young CDCs rejuvenate old animals as gauged by cardiac gene expression, heart function, exercise capacity, and systemic biomarkers.