gebhart/patrepeval huggingface dataset:
Total Knee Arthroplasty (1985)

The standard prosthesis for most arthritic conditions is a tricompartmental type. Patellar resurfacing should be done in most cases. The question of cruciate preservation or substitution is unresolved, and both types give equivalent clinical results. No advantage has been shown for left or right components. Correction of deformity occurs by soft-tissue release and ligament balancing, rather than by bone resection. Most primary replacements can be performed in this manner, but alignment is critical to the function and survival of a functioning arthroplasty. Most failures can be attributed to incorrect ligament balance or incorrect alignment. Cement fixation of the components has proved effective, and there is no immediate need for alternative methods such as bone ingrowth; new methods will have to prove themselves against the standard already established for cemented prostheses. Patellar complications such as fatigue fracture of the patellar bone now constitute the majority of problems following total knee arthroplasty.
Post operative analgesia after incisional infiltration of bupivacaine v/s bupivacaine with buprenorphine (2011)

Addition of buprenorphine to the local anesthetic significantly prolonged the time to first rescue analgesic requirement and the total consumption of rescue analgesic in 24 h, thus providing evidence in support of the existence of peripheral opioid receptors.
Theory of Imaging And Diffraction (2003)

In this review, theoretical approaches of imaging and diffraction in electron microscopy are introduced which allows the diffraction patterns and images to be treated with equal facility and emphasized the relationships between them. The coherent wave optics, incoherent wave imaging theory were introduced. The idea of Abbe theory was also introduced. Varoius phase contrast theories in small angle approximation were derived including the wave theory on Multi-component system.
The human VH3b gene subfamily is highly polymorphic. (1993)

Abstract We have previously shown that human antibody (Ab) directed against the capsular polysaccharide of the important bacterial pathogen, Haemophilus influenzae type b (Hib) is encoded by a small group of VH3 gene family members. The majority of anti-Hib PS Ab use members of the smaller VH3b subfamily. To examine directly the available human VH3 repertoire, we have used PCR to amplify and clone candidate germ-line VH3b H chain V region genes from two unrelated subjects from whom anti-Hib polysaccharide mAb had been previously obtained. A single functional VH3b germ-line gene was obtained from one subject. This gene is identical throughout the coding region to the previously identified gene 9.1. Twelve distinct VH3b germ-line sequences, 87.6-99.8% homologous to one another, were obtained from the second subject. One of these genes, LSG1.1, is also identical to the 9.1 germ-line gene, and a second, LSG6.1, is identical to a previously reported cDNA, M85. These germ-line VH3b genes are 82.7-94.1% homologous to rearranged anti-Hib PS VH3b segments obtained from these subjects. Our findings further demonstrate that considerable polymorphism of VH segments exists in the human population. Despite the presence of very highly homologous VH elements in the germ line, particular genes are highly conserved within the outbred human population.
Structural variation and missense mutation in SBDSassociated with Shwachman-Diamond syndrome (2014)

Our findings implicate genomic rearrangements in the pathogenesis of some cases of SDS and support patients lacking biallelic SBDS point mutations be tested for SV within the SBDS locus.
Additive antisense effects of different PNAs on the in vitro translation of the PML/RARalpha gene (1998)

The potential use of peptide nucleic acid (PNA) as a sequence-specific inhibitor of RNA translation is investigated in this report. Three different regions of the PML/RARalpha oncogene, including two AUG potential start codons, were studied as targets of translation inhibition by antisense PNA in a cell-free system. A PNA targeted to the second AUG start codon, which was shown previously to be able to suppress in vitro translation from that site completely, was used alone or in combination with another PNA directed to the first AUG, and a third PNA within the 5'-untranslated region (5'-UTR) of mRNA. When used alone, no PNA was able to completely block the synthesis of the PML/RARalpha protein. The 5'-UTR PNA was the most potent translation inhibitor when used as single agent. However, a near complete (>/=90%) specific inhibition of the PML/RARalpha gene was obtained when the three PNAs were used in combination, thus obtaining an additive antisense effect.
Nanostructuring and functionalizing polymers with cyclodextrins (2007)

We summarize our recent studies employing the cyclic starches called cyclodextrins (CDs) to both nanostructure and functionalize polymers. Two important structural characteristics of CDs are taken as advantages to achieve these goals. First the ability of CDs to form non-covalent inclusion complexes (ICs) with a variety of guest molecules, including many polymers, by threading and inclusion into their relatively hydrophobic interior cavities, which are roughly cylindrical with diameters of ∼0.5 to 1.0 nm for α-, β-, and γ-CD containing 6, 7, and 8 α-1,4-linked glucose units, respectively. When guest polymers are coalesced from the CD-ICs by removing their host CDs, they are observed to solidify with structures, morphologies, and even conformations that are distinct from bulk samples made from their solutions and melts. Molecularly mixed, intimate blends of two or more polymers that are normally immiscible can be obtained from their common CD-ICs, and the phase segregation of incompatible blocks can be controlled (suppressed or increased) in CD-IC coalesced block copolymers. In addition, additives may be more effectively delivered to polymers in the form of their soluble or crystalline CD-ICs or rotaxanes. Secondly, many –OH groups attached to the exterior rims of CDs, in addition to conferring water solubility, provide an opportunity to covalently bond them to polymers either during their syntheses or via post-polymerization reactions. Polymers containing CDs in their backbones or attached to their side chains are observed to more readily accept and retain additives, such as dyes, fragrances, etc. They may also be further reacted or treated through their CDs to cross-link and form networks or to form blends with other polymers having a propensity to thread through their attached CD cavities.
Developmental regulation of intestinal angiogenesis by indigenous microbes via Paneth cells (2002)

The adult mouse intestine contains an intricate vascular network. The factors that control development of this network are poorly understood. Quantitative three-dimensional imaging studies revealed that a plexus of branched interconnected vessels developed in small intestinal villi during the period of postnatal development that coincides with assembly of a complex society of indigenous gut microorganisms (microbiota). To investigate the impact of this environmental transition on vascular development, we compared the capillary networks of germ-free mice with those of ex-germ-free animals colonized during or after completion of postnatal gut development. Adult germ-free mice had arrested capillary network formation. The developmental program can be restarted and completed within 10 days after colonization with a complete microbiota harvested from conventionally raised mice, or with Bacteroides thetaiotaomicron, a prominent inhabitant of the normal mouse/human gut. Paneth cells in the intestinal epithelium secrete antibacterial peptides that affect luminal microbial ecology. Comparisons of germ-free and B. thetaiotaomicron-colonized transgenic mice lacking Paneth cells established that microbial regulation of angiogenesis depends on this lineage. These findings reveal a previously unappreciated mechanism of postnatal animal development, where microbes colonizing a mucosal surface are assigned responsibility for regulating elaboration of the underlying microvasculature by signaling through a bacteria-sensing epithelial cell.
Effect of Admixed Indium on Mercury Vapor Release from Dental Amalgam (1989)

The purpose of this study was to evaluate the effect of admixed indium on the amount of mercury vapor released from dental amalgam. We made amalgam specimens according to ADA Specification Number 1. We added various amounts (0-14% by weight) of indium to the alloy powder. We also tested the commercial amalgams Dispersalloy, Valiant, and Indiloy. Specimens were held at 37 degrees C and 100% humidity. We used a Jerome Mercury Vapor Analyzer (model 411) to measure the mercury vapor released. We analyzed the data by means of ANOVA and Student-Newman-Keuls procedures. Admixed indium significantly decreased the amount of mercury vapor released from dental amalgams; the period of the greatest effect on mercury vapor released was during the setting phase of amalgam; and dental amalgams with greater than or equal to 8% admixed indium released the least amount of mercury vapor.
Sodium Setpoint and Sodium Gradient: Influence on Plasma Sodium Change and Weight Gain (2010)

IDWG correlated with the sodium gradient and more strongly with delta PNa, suggesting the need for studying other outcomes, such as morbidity and mortality.
Immune Checkpoint inhibitors: An introduction to the next‐generation cancer immunotherapy (2015)

Activating the immune system to eliminate cancer cells and produce clinically relevant responses has been a long-standing goal of cancer research. Most promising therapeutic approaches to activating antitumor immunity include immune checkpoint inhibitors. Immune checkpoints are numerous inhibitory pathways hardwired in the immune system. They are critical for maintaining self-tolerance and modulating the duration and amplitude of physiological immune responses in peripheral tissues to minimize collateral tissue damage. Tumors regulate certain immune checkpoint pathways as a major mechanism of immune resistance. Because immune checkpoints are initiated by ligand-receptor interactions, blockade by antibodies provides a rational therapeutic approach. Although targeted therapies are clinically successful, they are often short-lived due to rapid development of resistance. Immunotherapies offer one notable advantage. Enhancing the cell-mediated immune response against tumor cells leads to generation of a long-term memory lymphocyte population patrolling the body to attack growth of any new tumor cells, thereby sustaining the therapeutic effects. Furthermore, early clinical results suggest that combination immunotherapies offer even more potent antitumor activity. This review is intended to provide an introduction to immune checkpoint inhibitors and discusses the scientific overview of cancer immunotherapy, mechanisms of the inhibitors, clinical pharmacology considerations, advances in combination therapies, and challenges in drug development.
Re-ranking of web image search results using a graph algorithm (2008)

We propose a method to improve the results of image search engines on the Internet to satisfy users who desire to see relevant images in the first few pages. The method re-ranks the results of text based systems by incorporating visual similarity of the resulting images. We observe that, together with many unrelated ones, results of text based systems include a subset of correct images, and this set is, in general, the largest one which has the most similar images compared to other possible subsets. Based on this observation, we present similarities of all images in a graph structure, and find the densest component that corresponds to the largest set of most similar subset of images. Then, to re-rank the results, we give higher priority to the images in the densest component, and rank the others based on their similarities to the images in the densest component. The experiments are carried out on 18 category of images from.
Percutaneous Intradiscal Radio-Frequency Thermocoagulation (1995)

A bipolar electrode and a radio-frequency alternating current permits percutaneous intradiscal thermocoagulation for destruction of a portion of disc material in a cadaveric spine.
Endothelial Cell Apoptosis Induced by Oxidized LDL Is Associated with the Down-regulation of the Cellular Caspase Inhibitor FLIP (1998)

Fas (CD-95/APO-1) is a death receptor that initiates an apoptotic signal when activated by its ligand, FasL. Normal vascular endothelial cells are resistant to Fas-mediated apoptosis though they express both Fas and FasL. Oxidized low density lipoprotein (OxLDL) or lysophosphatidylcholine (LPC), a major component of OxLDL, induces endothelial cell suicide by sensitizing endothelial cells to Fas-mediated apoptosis. Here, we show that endothelial cell apoptosis by OxLDL and LPC-C16:0 was dose-dependent and correlated with down-regulation of FLICE-inhibitory protein (FLIP), an intracellular caspase inhibitor. FLIP down-regulation also occurred when endothelial cells were treated with toxic doses of LPC-C18:0 or minimally modified low density lipoprotein (LDL). In contrast, FLIP was not down-regulated by native LDL, acetylated LDL, LPC-C12:0, cholesterol, or 7-ketocholesterol, which are not toxic to endothelial cells. The cytotoxicity of oxidized lipids was reversed by transfecting endothelial cells with a FLIP expression plasmid. The results demonstrate, for the first time, FLIP regulation under conditions that lead to pathological tissue destruction.
Syntheses of (R)- and (S)-2- and 6-Fluoronorepinephrine and (R)- and (S)-2- and 6-Fluoroepinephrine:  Effect of Stereochemistry on Fluorine-Induced Adrenergic Selectivities (2000)

Several routes to the enantiomers of fluoronorepinephrines (1) and fluoroepinephrines (2) were explored. A catalytic enantioselective oxazaborolidine reduction and a chiral (salen)Ti(IV) catalyzed asymmetric synthesis of silyl cyanohydrins proved efficacious in the key stereo-defining steps of two respective routes. Binding studies of the catecholamines with alpha(1)-, alpha(2)-, beta(1)-, and beta(2)-adrenergic receptors were examined. The assays confirmed that fluorine substitution had marked effects on the affinity of (R)-norepinephrine and (R)-epinephrine for adrenergic receptors, depending on the position of substitution. Thus, a fluoro substituent at the 2-position of (R)-norepinephrine and (R)-epinephrine reduced activity at both alpha(1)- and alpha(2)-receptors and enhanced activity at beta(1)- and beta(2)-receptors, while fluorination at the 6-position reduced activity at the beta(1)- and beta(2)-receptors. The effects of fluorine substitution on the S-isomers were less predictable.
Computed Tomographic Measurements of Airway Dimensions and Emphysema in Smokers (2000)

Chronic obstructive pulmonary disease (COPD) is characterized by the presence of airflow obstruction caused by emphysema or airway narrowing, or both. Low attenuation areas (LAA) on computed tomography (CT) have been shown to represent macroscopic or microscopic emphysema, or both. However CT has not been used to quantify the airway abnormalities in smokers with or without airflow obstruction. In this study, we used CT to evaluate both emphysema and airway wall thickening in 114 smokers. The CT measurements revealed that a decreased FEV(1) (%predicted) is associated with an increase of airway wall area and an increase of emphysema. Although both airway wall thickening and emphysema (LAA) correlated with measurements of lung function, stepwise multiple regression analysis showed that the combination of airway and emphysema measurements improved the estimate of pulmonary function test abnormalities. We conclude that both CT measurements of airway dimensions and emphysema are useful and complementary in the evaluation of the lung of smokers.
The optical fibre Sagnac interferometer: an overview of its principles and applications (2005)

The optical fibre Sagnac interferometer was first demonstrated over 25 years ago. Immediately its potential for gyroscopic measurements became apparent and since the first demonstration substantial research and development investment has evolved a diversity of rotation measuring instruments. The fibre Sagnac interferometer has, however, also ventured into unexpected domains. The fibre loop mirror has become the ubiquitous reflector. Sagnac-based intruder alarms, hydrophones, geophones and current measuring systems have emerged. The Sagnac interferometer has expanded from the rotation measuring instrument into a very versatile sensing tool. Indeed, it is arguably the most successful of optical fibre sensing technologies. In this paper, we review both the principles and applications of the fibre Sagnac interferometer. The background theory highlights the need to understand the conditions for reciprocity within the interferometer network. The applications range from the expected gyroscopes into novel hydrophone arrays and intruder detection systems. The basic interferometer is now well understood and the engineering required to realize useful and effective instruments has been carefully defined. Its versatility though continues to amaze even the most experienced practitioner and doubtless its applications potential will continue to expand.
Increased amyloid beta-peptide deposition in cerebral cortex as a consequence of apolipoprotein E genotype in late-onset Alzheimer disease. (1993)

Amyloid beta-peptide (A beta) deposition in senile plaques and cerebral vessels is a neuropathological feature of Alzheimer disease (AD). We examined the possibility that commonly observed variability in A beta deposition in late-onset AD might be related to apolipoprotein E genotype (APOE gene; the two most common alleles are 3 and 4), since APOE4 is a susceptibility gene for late-onset AD and apolipoprotein E interacts strongly with A beta in vitro. In an autopsy series of brains of late-onset AD patients, we found a strong association of APOE4 allele with increased vascular and plaque A beta deposits. Late-onset AD patients with one or two APOE4 alleles have a distinct neuropathological phenotype compared with patients homozygous for APOE3.
Lipids of Debaryomyces hansenii (1965)

Merdinger, Emanuel (Roosevelt University, Chicago, Ill.), and Edward M. Devine, Jr. Lipids of Debaryomyces hansenii. J. Bacteriol. 89:1488-1493. 1965.-The separation of neutral lipids and phospholipids from the lipid extract of Debaryomyces hansenii NRRL Y-1448 was accomplished by using a single column packed with silicic acid. The neutral lipids comprised 67%, and the phospholipids 33%, of the total lipid extract. Qualitative and quantitative characterization of the lipids was effected by various analytical procedures. Gas chromatography of the fatty acid methyl esters showed 11 acids, of which 59.7% were unsaturated. The most abundant among the unsaturated acids was the monounsaturated C18 acid (50.1%). Among the saturated acids, palmitic acid (23.5%) prevailed. Ratios of fatty acid to glycerol, phosphorus to glycerol, and phosphorus to fatty acid were ascertained for combined cuts from the neutral lipids and the phospholipids. The presence of ergosterol, stigmasterol, and an unidentified sterol was established by gas chromatography. Also present were saturated hydrocarbons containing 16 to 39 carbon atoms, C22 being the most prevalent.
Dielectrophoretic Characterization and Separation of Antibody-Coated Submicrometer Latex Spheres (1999)

The dielectrophoretic behavior of carboxylated 216-nm-diameter latex spheres has been characterized as a function of both medium conductivity and applied field frequency. Dielectrophoretic crossover measurements and analysis were used to characterize the dielectric properties of the particles. The particles were functionalized with antibodies using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDAC)-based coupling. Measurements indicated that the surface conductance of the native particles was 1.2 nS and that this reduced to a value of 0.7 nS after EDAC treatment and 0.25 nS after antibody coupling. Changes in the dielectrophoretic spectrum of the particles were exploited to demonstrate the principle of separation of unlabeled and protein-labeled particles. This demonstrates the potential for the development of new affinity separation systems based on ac electrokinetic methods.
Peptide sequencing and site-directed mutagenesis identify tyrosine-727 as the active site tyrosine of Saccharomyces cerevisiae DNA topoisomerase I. (1989)

Extensive digestion of the covalent intermediate between DNA and Saccharomyces cerevisiae DNA topoisomerase I with trypsin yields a 7-amino acid peptide covalently linked to DNA. Direct sequencing of the DNA-linked peptide identifies Tyr-727 as the active site tyrosine that forms an O4-phosphotyrosine bond with DNA when the enzyme cleaves a DNA phosphodiester bond. Site-directed mutagenesis of the cloned yeast TOP1 gene encoding the enzyme confirms the essentiality of Tyr-727 for the relaxation of supercoiled DNA by the enzyme. From amino acid sequence homology, Tyr-771 and -773 are readily identified as the active site tyrosines of Schizosaccharomyces pombe and human DNA topoisomerase I, respectively. Sequence comparison and site-directed mutagenesis also implicate Tyr-274 of vaccinia virus DNA topoisomerase as the active site residue. There appears to be a 70-amino acid domain near the carboxyl terminus of eukaryotic DNA topoisomerase I and vaccinia topoisomerase, within which the active site tyrosine resides.
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting (2015)

Exosomes are small vesicles that mediate cell-cell communication. They contain proteins, lipids and RNA, and evidence is accumulating that these molecules are specifically sorted for release via exosomes. We recently showed that endothelial-cell-produced exosomes promote angiogenesis in vivo in a small RNA-dependent manner. Recent deep sequencing studies in exosomes from lymphocytic origin revealed a broad spectrum of small RNAs. However, selective depletion or incorporation of small RNA species into endothelial exosomes has not been studied extensively. With next generation sequencing, we identified all known non-coding RNA classes, including microRNAs (miRNAs), small nucleolar RNAs, yRNAs, vault RNAs, 5p and 3p fragments of miRNAs and miRNA-like fragments. In addition, we mapped many fragments of messenger RNAs (mRNAs) and mitochondrial RNAs (mtRNAs). The distribution of small RNAs in exosomes revealed a considerable overlap with the distribution in the producing cells. However, we identified a remarkable enrichment of yRNA fragments and mRNA degradation products in exosomes consistent with yRNAs having a role in degradation of structured and misfolded RNAs in close proximity to endosomes. We propose that endothelial endosomes selectively sequester cytoplasmic RNA-degrading machineries taking part in gene regulation. The release of these regulatory RNAs via exosomes may have implications for endothelial cell-cell communication.
Physical activity and environmental enrichment regulate the generation of neural precursors in the adult mouse substantia nigra in a dopamine-dependent manner (2012)

We suggest dopamine being a key regulator for activity-induced generation of NG2-cells and oliogodendrocytes in the SN as a potentially relevant mechanism in endogenous nigral cellular plasticity.