gebhart/patrepeval huggingface dataset:
Modification of abnormal left ventricular diastolic properties by nifedipine in patients with hypertrophic cardiomyopathy. (1982)

The effect of nifedipine on left ventricular isovolumic relaxation and diastolic filling properties and systemic and left ventricular hemodynamics was studied in 15 patients with hypertrophic cardiomyopathy. After nidefipine (10 mg sublingually), the prolonged left ventricular isovolumic relaxation time assessed by echocardiography decreased from 112 +/- 26 to 83 +/- 23 msec (p less than 0.0001), and the left ventricular pressure decay as measured by time constant T improved from 63 +/- 20 to 49 +/- 11 msec (p less than 0.05). Left ventricular filling dynamics also improved as assessed by a return toward normal in the depressed peak rate of left ventricular diastolic filling (dimension change 72 +/- 37 to 101 +/- 39 mm/sec, p less than 0.01) and the peak rate of posterior wall thinning (47 +/- 31 to 68 +/- 36 mm/sec, p less than 0.001). These changes were accompanied by hemodynamic evidence of improved diastolic function shown as a decrease in left ventricular end-diastolic pressure and a downward shift in the left ventricular diastolic pressure-dimension relationship, suggesting improved left ventricular distensibility. After nifedipine, there was a slight increase in heart rate and a decrease in systemic ventricular distensibility. After nifedipine, there was a slight increase in heart rate and a decrease in systemic arterial blood pressure, and no depression of the left ventricular percent fractional shortening or cardiac index. These data indicate that abnormal left ventricular relaxation and diastolic filling rates in hypertrophic cardiomyopathy are dynamic and favorably modified by nifedipine, and that this effect is not related to a depression of left ventricular systolic function.
Neutral endopeptidase promotes phorbol ester-induced apoptosis in prostate cancer cells by inhibiting neuropeptide-induced protein kinase C delta degradation. (2000)

Phorbol esters induce apoptosis in androgen-sensitive LNCaP cells, which express neutral endopeptidase (NEP), but not in androgen-independent prostate cancer (PC) cells, which lack NEP expression. We investigated the role of NEP in PC cell susceptibility to 12-O-tetradecanoylphorbol-13-acetate (TPA). Western analysis showed that expression of NEP and protein kinase Cdelta (PKCdelta) correlated with PC cell sensitivity to TPA-induced growth arrest and apoptosis in LNCaP cells and in TSU-Prl cells expressing an inducible wild-type NEP protein. Inhibition of NEP enzyme activity using the specific NEP inhibitor CGS24592, or inhibition of PKCdelta using Rottlerin at concentrations that inhibit PKCdelta but not PKCalpha, significantly inhibited TPA-induced growth inhibition and cell death. Furthermore, pulse-chase experiments showed PKCdelta is stabilized in LNCaP cells and in TSU-Pr1 cells overexpressing wild-type NEP compared with PC cells lacking NEP expression. This results from NEP inactivation of its neuropeptide substrates (bombesin and endothelin-1), which in the absence of NEP stimulate cSrc kinase activity and induce rapid degradation of PKCdelta protein. These results indicate that expression of enzymatically active NEP by PC cells is necessary for TPA-induced apoptosis, and that NEP inhibits neuropeptide-induced, cSrc-mediated PKCdelta degradation.
Mapping EBNA-1 Domains Involved in Binding to Metaphase Chromosomes (1999)

The Epstein-Barr virus (EBV) genome can persist in dividing human B cells as multicopy circular episomes. Viral episomes replicate in synchrony with host cell DNA and are maintained at a relatively constant copy number for a long time. Only two viral elements, the replication origin OriP and the EBNA-1 protein, are required for the persistence of viral genomes during latency. EBNA-1 activates OriP during the S phase and may also contribute to the partition and/or retention of viral genomes during mitosis. Indeed, EBNA-1 has been shown to interact with mitotic chromatin. Moreover, viral genomes are noncovalently associated with metaphase chromosomes. This suggests that EBNA-1 may facilitate the anchorage of viral genomes on cellular chromosomes, thus ensuring proper partition and retention. In the present paper, we have investigated the chromosome-binding activity of EBV EBNA-1, herpesvirus papio (HVP) EBNA-1, and various derivatives of EBV EBNA-1, fused to a variant of the green fluorescent protein. The results show that binding to metaphase chromosomes is a common property of EBV and HVP EBNA-1. Further studies indicated that at least three independent domains (CBS-1, -2, and -3) mediate EBNA-1 binding to metaphase chromosomes. In agreement with the anchorage model, two of these domains mapped to a region that has been previously demonstrated to be required for the long-term persistence of OriP-containing plasmids.
In Vivo Labeling of Serum Albumin for PET (2014)

The distribution and local accumulation of serum albumin can be noninvasively visualized and quantified by (18)F-AlF-NEB and (64)Cu-NEB PET. The simple labeling and broad applications make these imaging probes attractive for clinical translation.
Bacteriology of sputum in cystic fibrosis: evaluation of dithiothreitol as a mucolytic agent (1980)

Liquefaction and homogenization have been recommended to ensure accurate, representative sputum cultures. We evaluated dithiothreitol (DTT) as mucolytic agent for culturing sputum samples obtained from 79 cystic fibrosis (CF) patients. Liquefaction with DTT was not superior to direct plating of specimens for routine qualitative cultures. Unliquefied sputum cultures failed to direct 3 of 47 Pseudomonas aeruginosa isolates; DTT-treated specimens missed 5 of 13 Candida albicans isolates. Neither treated nor untreated sputum cultures were completely successful in detecting Staphylococcus aureus or Enterobacteriaceae. Since Haemophilus influenzae was recovered from only two qualitative cultures, we could not evaluate the effect of DTT on the receovery of this organism. However, 27 of 29 strains of H. influenzae were inhibited by concentrations of DTT near the recommended final working concentration of 50 micrograms/ml, suggesting that liquefaction might impair isolation of this organism. Liquefaction with DTT permitted quantitative cultures of CF sputum. The predominant pathogen in our CF population was P. aeruginosa; 37 of 43 (86%) patients were colonized with this organism. Median densities of rough and mucoid strains were 3.2 x 10(7) and 4.3 x 10(7) colony-forming units per ml, respectively. Previous oral antistaphylococcal therapy may have accounted for the observed low density of S. aureus (mean density, 3.5 x 10(3) colony-forming units per ml). We conclude that DTT treatment does not improve recovery of organisms from qualitative cultures but does facilitate quantitative studies of S. aureus and P. aeruginosa in CF sputum.
Myc-mediated apoptosis requires wild-type p53 in a manner independent of cell cycle arrest and the ability of p53 to induce p21waf1/cip1. (1994)

Deregulated expression of the c-myc proto-oncogene can lead to apoptosis under certain physiological conditions. By introducing a conditionally active Myc allele into primary embryo fibroblasts null for p53, and into fibroblasts without endogenous p53 expression but ectopically expressing a temperature-sensitive p53 allele, we show that expression of wild-type p53 is required for susceptibility to Myc-mediated apoptosis. Although ectopic expression of wild-type p53 blocked cells in the G1 phase of the cell cycle, G1 arrest by isoleucine starvation, in a manner independent of p53, did not confer susceptibility to apoptosis. Thus, growth arrest per se is not sufficient to induce Myc-mediated apoptosis; instead, a property intrinsic to p53 is specifically required. Moreover, apoptosis did not require induction of p53 target proteins, including the cyclin-dependent kinase inhibitor p21waf1/cip1. Therefore, the role of p53 in apoptosis may be distinct from its role in cell cycle arrest.
Up‐regulation of human telomerase catalytic subunit during gastric carcinogenesis (1999)

BACKGROUND Telomerase activation is thought to be essential for the stabilization of telomere length, through which immortalization and oncogenesis are achieved, but little is known about the regulation of telomerase in human gastric carcinoma cells. METHODS A total of 27 primary gastric tumors, 29 cases of intestinal metaplasia, and 30 cases of normal mucosa, as well as 8 gastric carcinoma cell lines, were examined for the relation between telomerase activation and gastric carcinogenesis. Telomerase activity was detected by telomeric repeat amplification protocol, and the expression of each telomerase subunit was evaluated by Northern blot analysis or reverse transcriptase–-polymerase chain reaction. RESULTS Telomerase activity was found in all 8 gastric carcinoma cell lines and in 25 of 27 gastric carcinoma tissue samples (93%), and weakly observed in 11 of 29 gastric intestinal metaplasia samples (38%). None of 30 normal gastric tissue samples displayed telomerase activity. The mRNA expression of human telomerase catalytic subunit (hTERT) was up-regulated in 26 of 26 tumor tissue samples (100%) and in 19 of 24 intestinal metaplasia (79%) in which telomerase activity was weak or negative. Normal gastric mucosa expressed the telomerase gene, albeit at low levels. In contrast to hTERT, human telomerase RNA component and human telomerase-associated protein expression did not parallel telomerase activity, which was independent of tumor stage and histology. CONCLUSIONS hTERT expression is up-regulated during an early stage in the carcinogenic process, and telomerase activation may be a critical step in gastric carcinogenesis. Cancer 1999;86:559–65. © 1999 American Cancer Society.
Dopant Concentration–Porosity–Li-Ion Conductivity Relationship in Garnet-Type Li5+2xLa3Ta2–xYxO12 (0.05 ≤ x ≤ 0.75) and Their Stability in Water and 1 M LiCl (2015)

Highly Li-ion conductive Y-doped garnet-type Li5+2xLa3Ta2-xYxO12 (0.05 ≤ x ≤ 0.75) were studied to understand the effects of yttrium- and lithium-doping on crystal structure, porosity, and Li-ion conductivity using (7)Li MAS NMR, electrochemical ac impedance spectroscopy, and scanning electron microscopy (SEM), as well as ex situ and in situ powder X-ray diffraction (PXRD) to further explore the potential application of garnets in all-solid-state Li-ion batteries. Solid-state (7)Li MAS NMR studies showed an increase in the Li-ion mobility as a function of Y- and Li-doping in Li5+2xLa3Ta2-xYxO12, which is consistent with the results from ac impedance spectroscopy. The SEM studies on sintered pellets indicated a systematic decrease in porosity and an increase in sinterability as the Y- and Li-doping levels increase in Li5+2xLa3Ta2-xYxO12. These results are consistent with the calculated porosity and densities using the Archimedes method. Using the variable-temperature in situ PXRD in the temperature range of 30-700 °C, a thermal expansion coefficient of 7.25 × 10(-6) K(-1) was observed for Li6La3Ta1.5Y0.5O12. To further explore the possibility of a new application for the Li-stuffed garnets, the stability of these materials in aqueous LiCl solution was also studied. A high degree of structural stability was observed in these materials upon 1 M LiCl treatment, making them suitable candidates for further studies as protective layers for lithium electrodes in aqueous lithium batteries.
Detection and Printability of Shifter Defects in Phase-Shifting Masks II. Defocus Characteristics (1992)

One drawback of phase-shifting lithography lies in the high printability of shifter defects. A transparent object on masks (shifter defects) produces lead or lag of the optical phase, which causes an unique printability with respect to focus level. We investigated the printability of shifter defects on phase-shifting masks in out-of-focus conditions. Two types of shifter defects related to the phase angle were investigated: (a) a phase error which is a shifter layer having an incorrect thickness and (b) a phase angle defect which is a defect having a phase angle other than 180°. Phase error causes asymmetrical pattern profiles. It was found that the illumination coherence factor (σ) has a strong influence on the allowance of the shifter thickness (phase error). The effect of phase angle defects located in a large clear area and in small features was investigated. The phase angle defects have an optimum focal plane other than the best focal plane. A defect with a low phase shift located in fine patterns has high printability. In order to establish a mask fabrication process for production-worthy phase-shifting masks, further investigation in the detection of low phase angle shifter defects is necessary.
Resonance Raman imaging of macular pigment distributions in the human retina (2008)

We describe resonance Raman imaging (RRI) of macular pigment (MP) distributions in the living human eye. MP consists of the antioxidant carotenoid compounds lutein and zeaxanthin, is typically present in high concentrations in the healthy human macula relative to the peripheral retina, and is thought to protect this important central region from age-related macular degeneration. We demonstrate that RRI is capable of quantifying and imaging the spatially strongly varying MP distribution in the human retina. Using laser excitation of the MP molecules at 488nm, and sequential camera detection of light emitted back from the retina at the MP's strongest Raman peak position and at an off-peak position, RRI maps of MP are obtained at a resolution below 50microm within a fraction of a second per exposure. RRI imaging can be carried out with undilated pupils and provides a highly molecule-specific diagnostic imaging approach for MP distributions in human subjects.
High-dimensional signature compression for large-scale image classification (2011)

We address image classification on a large-scale, i.e. when a large number of images and classes are involved. First, we study classification accuracy as a function of the image signature dimensionality and the training set size. We show experimentally that the larger the training set, the higher the impact of the dimensionality on the accuracy. In other words, high-dimensional signatures are important to obtain state-of-the-art results on large datasets. Second, we tackle the problem of data compression on very large signatures (on the order of 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">5</sup> dimensions) using two lossy compression strategies: a dimensionality reduction technique known as the hash kernel and an encoding technique based on product quantizers. We explain how the gain in storage can be traded against a loss in accuracy and/or an increase in CPU cost. We report results on two large databases - ImageNet and a dataset of lM Flickr images - showing that we can reduce the storage of our signatures by a factor 64 to 128 with little loss in accuracy. Integrating the decompression in the classifier learning yields an efficient and scalable training algorithm. On ILSVRC2010 we report a 74.3% accuracy at top-5, which corresponds to a 2.5% absolute improvement with respect to the state-of-the-art. On a subset of 10K classes of ImageNet we report a top-1 accuracy of 16.7%, a relative improvement of 160% with respect to the state-of-the-art.
ENHANCED GRANULYSIN mRNA EXPRESSION IN URINARY SEDIMENT IN EARLY AND DELAYED ACUTE RENAL ALLOGRAFT REJECTION (2004)

The authors' data illustrate that the noninvasive kinetic mRNA expression measurement of defined markers in urinary cells of renal allograft recipients allows the early noninvasive detection of ongoing aRx.
Climate change and population health (2008)

Climate change has already affected many aspects of the earth ecological system. Population health should be one of the most concerned. However, the relationship between climate and population health, including both chronic diseases and infectious diseases, is far from clear. A critical review of previous studies on the relationship between climate change and population health has been conducted with focuses on research methodology, including study population, study design and statistical method, and their results. Further research trends on climate change and population health will be addressed based on the critical review.
Modification of the Tet-On regulatory system prevents the conditional-live HIV-1 variant from losing doxycycline-control (2006)

The loss of dox-control observed upon evolution of the dox-dependent HIV-1 variant was effectively blocked by modification of the Tet-On regulatory system.
Regional Clustering of Shared Neutralization Determinants on Primary Isolates of Clade C Human Immunodeficiency Virus Type 1 from South Africa (2002)

Clade C is one of the most prevalent genetic subtypes of human immunodeficiency virus type 1 (HIV-1) in the world today and one of the least studied with respect to neutralizing antibodies. Most information on HIV-1 serology as it relates to neutralization is derived from clade B. Clade C primary isolates of HIV-1 from South Africa and Malawi were shown here to resemble clade B isolates in their resistance to inhibition by soluble CD4 and their sensitivity to neutralization by human monoclonal antibody immunoglobulin G1b12 and, to a lesser extent, 2F5. Unlike clade B isolates, however, all 16 clade C isolates examined resisted neutralization by 2G12. Infection with clade C HIV-1 in a cohort of female sex workers in South Africa generated antibodies that neutralized the autologous clade C isolate and T-cell-line-adapted (TCLA) strains of clade B. Neutralization of clade B TCLA strains was much more sensitive to the presence of autologous gp120 V3 loop peptides compared to the neutralization of clade C isolates in most cases. Thus, the native structure of gp120 on primary isolates of clade C will likely pose a challenge for neutralizing antibody induction by candidate HIV-1 vaccines much the same as it has for clade B. The autologous neutralizing antibody response following primary infection with clade C HIV-1 in South Africa matured slowly, requiring at least 4 to 5 months to become detectable. Once detectable, extensive cross-neutralization of heterologous clade C isolates from South Africa was observed, suggesting an unusual degree of shared neutralization determinants at a regional level. This high frequency of cross-neutralization differed significantly from the ability of South African clade C serum samples to neutralize clade B isolates but did not differ significantly from results of other combinations of clade B and C reagents tested in checkerboard assays. Notably, two clade C serum samples obtained after less than 2 years of infection neutralized a broad spectrum of clade B and C isolates. Other individual serum samples showed a significant clade preference in their neutralizing activity. Our results suggest that clades B and C are each comprised of multiple neutralization serotypes, some of which are more clade specific than others. The clustering of shared neutralization determinants on clade C primary HIV-1 isolates from South Africa suggests that neutralizing antibodies induced by vaccines will have less epitope diversity to overcome at a regional level.
Loss of heterozygosity (LOH), malignancy grade and clonality in microdissected prostate cancer (1999)

The aim of the present study was to find out whether increasing malignancy of prostate carcinoma correlates with an overall increase of loss of heterozygosity (LOH), and whether LOH typing of microdissected tumour areas can help to distinguish between multifocal or clonal tumour development. In 47 carcinomas analysed at 25 chromosomal loci, the overall LOH rate was found to be significantly lower in grade 1 areas (2.2%) compared with grade 2 (9.4%) and grade 3 areas (8.3%, P = 0.007). A similar tendency was found for the mean fractional allele loss (FAL, 0.043 for grade 1, 0.2 for grade 2 and 0.23 for grade 3, P = 0.0004). Of 20 tumours (65%) with LOH in several microdissected areas, 13 had identical losses at 1-4 loci within two or three areas, suggesting clonal development of these areas. Markers near RB, DCC, BBC1, TP53 and at D13S325 (13q21-22) showed higher loss rates in grades 2 and 3 (between 25% and 44.4%) compared with grade 1 (0-6.6%). Tumour-suppressor genes (TSGs) near these loci might, thus, be important for tumour progression. TP53 mutations were detected in 27%, but BBC1 mutations in only 7%, of samples with LOH. Evaluation of all 25 loci in every tumour made evident that each prostate cancer has its own pattern of allelic losses.
Spectral analysis of the full gravity tensor (1992)

The five independent components Γ&lt;inf&gt;xz&lt;/inf&gt;, Γ&lt;inf&gt;yz&lt;/inf&gt;, Γ&lt;inf&gt;zz&lt;/inf&gt;, Γ&lt;inf&gt;xx&lt;/inf&gt; − Γ&lt;inf&gt;yy&lt;/inf&gt;, and Γ&lt;inf&gt;xy&lt;/inf&gt;, of the gravity tensor are measurable by gradiometers. When grouped into {Γ&lt;inf&gt;zz&lt;/inf&gt;}, {Γ&lt;inf&gt;xz&lt;/inf&gt;, Γ&lt;inf&gt;yz&lt;/inf&gt;} and {Γ&lt;inf&gt;xx&lt;/inf&gt; - Γ&lt;inf&gt;yy&lt;/inf&gt;, 2Γ&lt;inf&gt;xy&lt;/inf&gt;} and expanded into an infinite series of pure-spin spherical harmonic tensors, simple eigenvalue connections can be derived between these three sets and the spherical harmonic expansion of the gravity potential. The three eigenvalues are (&lt;it&gt;n&lt;/it&gt;+ 1)(&lt;it&gt;n&lt;/it&gt;+ 2), −(&lt;it&gt;n&lt;/it&gt;+ 2)√&lt;it&gt;n&lt;/it&gt;(&lt;it&gt;n&lt;/it&gt;+ 1) and √(&lt;it&gt;n&lt;/it&gt; − 1)&lt;it&gt;n&lt;/it&gt;(&lt;it&gt;n&lt;/it&gt;+ 1)(&lt;it&gt;n&lt;/it&gt;+ 2). This result permits an easy analytical incorporation of all measurable tensor components into a spectral signal and noise analysis of gravity quantities on a sphere. Analogous relations also exist for a 2-D Fourier (flat earth) expansion of these three sets. An additional advantageous feature of the set {Γ&lt;inf&gt;xx&lt;/inf&gt; - Γ&lt;inf&gt;yy&lt;/inf&gt;, 2Γ&lt;inf&gt;xy&lt;/inf&gt;}, besides the simple eigenvalue relation, is its invariance with respect to small position uncertainties, e.g., of the trajectory of the satellite or airplane carrying the gradiometer. Hence a complete framework exists in terms of the eigenvectors of all operators connecting the zeroth, first, and second derivatives of the gravitational potential. At the same time the results facilitate the planning of gradiometer missions and their data analysis.