gebhart/patrepeval huggingface dataset:
Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development. (1996)

Recently our laboratory described an efficient method for generating retroviral provirus insertions in the zebrafish germ line, and we showed that provirus insertions induce embryonic mutations at a frequency of roughly one mutant per 70 insertions. To date we have isolated four insertional mutants and, using the proviruses as a molecular tag, have cloned the genes disrupted in three of them. The proviruses in all three mutants lie within or just 5' of the first coding exon, point in the opposite transcriptional orientation from the gene, and disrupt transcription. Here we present a molecular characterization of two genes identified by this method and describe the associated mutant phenotypes. The pescadillo (pes) gene is predicted to encode a protein of 582 amino acids with no recognizable functional motifs, which is highly conserved from yeast to humans. pes mRNA is expressed widely and dynamically during the first 3 days of embryogenesis. Prominent sites of expression are the eyes and optic tectum on day 1, the fin buds, liver primordium, and gut on day 2, and the branchial arches on day 3. Beginning at day 3 of embryogenesis, pes mutant embryos exhibit small eyes, a reduced brain and visceral skeleton, shortened fins, and a lack of expansion of the liver and gut, and then die on the sixth day of development. The dead eye (dye) gene encodes a protein of 820 amino acids that is homologous to genes of unknown function in human, mouse, and Xenopus, and that has weak homology with the yeast NIC96 (nucleoporin-interacting component) gene. dye mutants can be recognized on day 2 of embryogenesis by the presence of necrotic cells in the tectum and eyes. dye mutants die on day 5 of development. These results demonstrate the power of insertional mutagenesis in zebrafish for rapidly finding and characterizing novel genes essential for embryonic development. Using our current methodology, we estimate that our laboratory could screen approximately 25,000 insertions in 2-3 years, identifying perhaps 250-350 embryonic lethal genes. Assuming that all genes are accessible to proviral insertion, the wider application of this approach could lead to the rapid identification of the majority of genes that are required for embryonic development of this vertebrate.
Small-Animal PET of Steroid Hormone Receptors Predicts Tumor Response to Endocrine Therapy Using a Preclinical Model of Breast Cancer (2012)

Using small-animal PET, a profile was identified that distinguished fulvestrant-sensitive from fulvestrant-resistant ERα(+)/PR(+) tumors before changes in tumor size. This work demonstrates that imaging baseline tumoral (18)F-FES uptake and initial changes in (18)F-FFNP uptake in a noninvasive manner is a potentially useful strategy to identify responders and nonresponders to endocrine therapy at an early stage.
Thermal Stability of Hydroxyl Groups on a Well-Defined Silica Surface (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThermal Stability of Hydroxyl Groups on a Well-Defined Silica SurfaceOfer Sneh and Steven M. GeorgeCite this: J. Phys. Chem. 1995, 99, 13, 4639–4647Publication Date (Print):March 1, 1995Publication History Published online1 May 2002Published inissue 1 March 1995https://pubs.acs.org/doi/10.1021/j100013a039https://doi.org/10.1021/j100013a039research-articleACS PublicationsRequest reuse permissionsArticle Views2495Altmetric-Citations192LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
The Dutch Presence in the Teutonic Order (2006)

eutonlC \....Ir erTraditionally, in Dutch historiography great emphasis has been placed on the "civil" character of the Netherlands, with their growth and development as a Aourishing trading nation in the 17 1h century.For that reason , one sometimes forgets that for a long time the Dutch territories belonged to the G erman Empire and therefore they also have a continental and aristocratic feudal past.The "chivalrous" relationship between the Netherla nds and Latvia, or Livonia as it was known in the Middle Ages, described below, of ten co nstitutes a relatively unknown chapter of that history.In the 11 1h and 12 1h centuries, the diocese of Utrecht, which covered almost two thirds of the current Dutc h territory, featured amongst the most important spiritual principalities of the Empire.This position of power meant that the bishop and his vassals of ten had to accompany the German Emperor on Crusades.This in turn implied that they were supporting the knightly orders in their territory with considerable donations.This enabled the Teutonic Order to establish a large House in the city of Utrecht and also numerous smaller Houses spread over the entire diocese.From these Houses they tried to supply their fortresses and convents in the Holy Land and elsewhere, both with money and personnel.The German House in Utrec ht was to become the centre of its own bailiwick or administrative district: one of the twelve bailiwicks th at were established in the German Empire outside the Order's states of Prussia and Livonia.The Livonian Branch of the Teutonic Order Beyond In the late Middle Ages, Livonia, in the broad sense, more or less covered the territory of the actual states of Latvia and Estonia.It comprised of hve spiritual territories, the Teutonic Order being the largest and most powerful.However, unlike the situation in Prussia, the Order never succeeded in fully imposing its will upon the other territories (the archbishopric of Riga and the dioceses of Dorpat (Tartu), sel-Wiek and Courland).In addition to that, the Order had to reckon with the powerful Hanseatic towns of Riga and Reval (Tallinn) which were dominated by German merchants.The Teutonic Order, which had been founded near Acre in Palestine in 1190 with the purpose of defending Christianity and hel ping to retake the Holy Se pulchre, was traditional borders
Interrelationships between the Human Alveolar Macrophage and Alpha-1-Antitrypsin (1973)

Alveolar macrophages lavaged from human lungs contain protease activity at an optimum pH of 3.0 and possibly a lesser peak of activity at pH 5.5. Protease activity measured at pH 4.1 is inhibited by purified alpha-1-antitrypsin. Fluorescent antibody studies of human alveolar macrophages showed that alpha-1-antitrypsin is present in normal alveolar macrophages. In addition, macrophages from a patient with a homozygous deficiency of alpha-1-antitrypsin exhibited less fluorescence when incubated in autologous serum than the same macrophages incubated in normal serum. Macrophages from normal subjects showed maximal fluorescence when removed from the lung and additional incubation with serum did not increase fluorescence. These results implicate the human alveolar macrophage as a possible source of an enzyme that may cause emphysema in patients deficient in alpha-1-antitrypsin. They also show that alpha-1-antitrypsin has access to the alveolus in normal subjects.
7 × 7 Reconstruction on Si(111) Resolved in Real Space (1983)

The 7 \ifmmode\times\else\texttimes\fi{} 7 reconstruction on Si(111) was observed in real space by scanning tunneling microscopy. The experiment strongly favors a modified adatom model with 12 adatoms per unit cell and an inhomogeneously relaxed underlying top layer.
Color quality scale (2010)

The color rendering index CRI has been shown to have deficiencies when applied to white light-emitting-diode-based sources. Furthermore, evidence suggests that the restricted scope of the CRI unnecessarily penalizes some light sources with desirable color qualities. To solve the problems of the CRI and include other dimensions of color quality, the color quality scale CQS has been developed. Although the CQS uses many of elements of the CRI, there are a number of fundamental differences. Like the CRI, the CQS is a test-samples method that compares the appearance of a set of reflective samples when illuminated by the test lamp to their appearance under a reference illuminant. The CQS uses a larger set of reflective samples, all of high chroma, and combines the color differences of the samples with a root mean square. Additionally, the CQS does not penalize light sources for causing increases in the chroma of object colors but does penalize sources with smaller rendered color gamut areas. The scale of the CQS is converted to span 0-100, and the uniform object color space and chromatic adaptation transform used in the calculations are updated. Supplementary scales have also been developed for expert users.
P‐172L: Late‐News Poster: Highly Stable Integrated Gate Driver Circuit using a‐Si TFT with Dual Pull‐down Structure (2005)

Abstract This is the first report on real XGA AMLCD for NBPC with highly stable integrated gate driver circuits using a‐Si TFT. The new circuit has been designed with dual‐pull down structure for high reliability and has been working for over 2,000 hours at 60°C, which is sufficient working time for normal driving conditions.
Pulmonary metastases in children and adolescents with well-differentiated thyroid cancer. (1998)

Complete response of pulmonary metastases after (131)I therapy is difficult to achieve. A partial response with reduction of metastatic disease is possible and, in general, the patients had a good quality of life with no further disease progression and a low mortality rate.
Basso Mouse Scale for Locomotion Detects Differences in Recovery after Spinal Cord Injury in Five Common Mouse Strains (2006)

Genetically engineered mice are used extensively to examine molecular responses to spinal cord injury (SCI). Inherent strain differences may confound behavioral outcomes; therefore, behavioral characterization of several strains after SCI is warranted. The Basso, Beattie, Bresnahan Locomotor Rating Scale (BBB) for rats has been widely used for SCI mice, but may not accurately reflect their unique recovery pattern. This study's purpose was to develop a valid locomotor rating scale for mice and to identify strain differences in locomotor recovery after SCI. We examined C57BL/6, C57BL/10, B10.PL, BALB/c, and C57BL/6x129S6 F1 strains for 42 days after mild, moderate, and severe contusive SCI or transection of the mid thoracic spinal cord. Contusions were created using the Ohio State University electromagnetic SCI device which is a displacement-driven model, and the Infinite Horizon device, which is a force-driven model. Attributes and rankings for the Basso Mouse Scale for Locomotion (BMS) were determined from frequency analyses of seven locomotor categories. Mouse recovery differed from rats for coordination, paw position and trunk instability. Disagreement occurred across six expert raters using BBB (p < 0.05) but not BMS to assess the same mice. BMS detected significant differences in locomotor outcomes between severe contusion and transection (p < 0.05) and SCI severity gradations resulting from displacement variations of only 0.1 mm (p < 0.05). BMS demonstrated significant face, predictive and concurrent validity. Novice BMS raters with training scored within 0.5 points of experts and demonstrated high reliability (0.92-0.99). The BMS is a sensitive, valid and reliable locomotor measure in SCI mice. BMS revealed significantly higher recovery in C57BL/10, B10.PL and F1 than the C57BL/6 and BALB/c strains after moderate SCI (p < 0.05). The differing behavioral response to SCI suggests inherent genetic factors significantly impact locomotor recovery and must be considered in studies with inbred or genetically engineered mouse strains.
Diversity regularized machine (2011)

Ensemble methods, which train multiple learners for a task, are among the state-of-the-art learning approaches. The diversity of the component learners has been recognized as a key to a good ensemble, and existing ensemble methods try different ways to encourage diversity, mostly by heuristics. In this paper, we propose the diversity regularized machine (DRM) in a mathematical programming framework, which efficiently generates an ensemble of diverse support vector machines (SVMs). Theoretical analysis discloses that the diversity constraint used in DRM can lead to an effective reduction on its hypothesis space complexity, implying that the diversity control in ensemble methods indeed plays a role of regularization as in popular statistical learning approaches. Experiments show that DRM can significantly improve generalization ability and is superior to some state-of-the-art SVM ensemble methods.
The roles of BMP and IL-3 signaling pathways in the control of hematopoietic stem cells in the mouse embryo (2010)

During mouse ontogeny, the first adult-type hematopoietic stem cells (HSC) are autonomously generated at mid-gestation in the AGM (Aorta-Gonad-Mesonephros) region. Successively present in different anatomical sites where they will expand, HSCs will finally colonize the bone marrow (BM) where they will reside during the entire adult life. In the bone marrow, both HSC self-renewal and differentiation are controlled at cellular and molecular levels by interactions with the stromal microenvironment. So far, very little is known about the extracellular factors involved in the regulation of embryonic HSC emergence, survival and expansion. In the present review, we outline the BMP and IL-3 signaling pathways that are critical for the growth and potential of embryonic HSCs. We will also discuss how these pathways might be integrated with the ones of Notch and Mpl/thrombopoietin, also identified as important key regulators of AGM HSC activity.
The state of the art in distributed query processing (2000)

Distributed data processing is becoming a reality. Businesses want to do it for many reasons, and they often must do it in order to stay competitive. While much of the infrastructure for distributed data processing is already there (e.g., modern network technology), a number of issues make distributed data processing still a complex undertaking: (1) distributed systems can become very large, involving thousands of heterogeneous sites including PCs and mainframe server machines; (2) the state of a distributed system changes rapidly because the load of sites varies over time and new sites are added to the system; (3) legacy systems need to be integrated—such legacy systems usually have not been designed for distributed data processing and now need to interact with other (modern) systems in a distributed environment. This paper presents the state of the art of query processing for distributed database and information systems. The paper presents the “textbook” architecture for distributed query processing and a series of techniques that are particularly useful for distributed database systems. These techniques include special join techniques, techniques to exploit intraquery paralleli sm, techniques to reduce communication costs, and techniques to exploit caching and replication of data. Furthermore, the paper discusses different kinds of distributed systems such as client-server, middleware (multitier), and heterogeneous database systems, and shows how query processing works in these systems.
Tissue Classification with Gene Expression Profiles (2000)

Constantly improving gene expression profiling technologies are expected to provide understanding and insight into cancer-related cellular processes. Gene expression data is also expected to significantly aid in the development of efficient cancer diagnosis and classification platforms. In this work we examine three sets of gene expression data measured across sets of tumor(s) and normal clinical samples: The first set consists of 2,000 genes, measured in 62 epithelial colon samples (Alon et al., 1999). The second consists of ≈ 100,000 clones, measured in 32 ovarian samples (unpublished extension of data set described in Schummer et al. (1999)). The third set consists of ≈ 7,100 genes, measured in 72 bone marrow and peripheral blood samples (Golub et al., 1999). We examine the use of scoring methods, measuring separation of tissue type (e.g., tumors from normals) using individual gene expression levels. These are then coupled with high-dimensional classification methods to assess the classification power of complete expression profiles. We present results of performing leave-one-out cross validation (LOOCV) experiments on the three data sets, employing nearest neighbor classifier, SVM (Cortes and Vapnik, 1995), AdaBoost (Freund and Schapire, 1997) and a novel clustering-based classification technique. As tumor samples can differ from normal samples in their cell-type composition, we also perform LOOCV experiments using appropriately modified sets of genes, attempting to eliminate the resulting bias. We demonstrate success rate of at least 90% in tumor versus normal classification, using sets of selected genes, with, as well as without, cellular-contamination-related members. These results are insensitive to the exact selection mechanism, over a certain range.
Preparation of 1,3-Oxathiolane-2-thiones by the Reaction of Oxirane and Carbon Disulfide (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation of 1,3-Oxathiolane-2-thiones by the Reaction of Oxirane and Carbon DisulfideNobuhiro Kihara, Yuichi Nakawaki, and Takeshi EndoCite this: J. Org. Chem. 1995, 60, 2, 473–475Publication Date (Print):January 1, 1995Publication History Published online1 May 2002Published inissue 1 January 1995https://pubs.acs.org/doi/10.1021/jo00107a034https://doi.org/10.1021/jo00107a034research-articleACS PublicationsRequest reuse permissionsArticle Views1045Altmetric-Citations100LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Collagen as an Implantable Material in Medicine and Dentistry (2002)

Collagen is a highly versatile material, extensively used in the medical, dental, and pharmacological fields. Collagen is capable of being prepared into cross-linked compacted solids or into lattice-like gels. Resorbable forms of collagen have been used to dress oral wounds, for closure of graft and extraction sites, and to promote healing. Collagen-based membranes also have been used in periodontal and implant therapy as barriers to prevent epithelial migration and allow cells with regenerative capacity to repopulate the defect area. It has been hypothesized that membrane regenerative techniques facilitate the natural biological potential by creating a favorable environment for periodontal and peri-implant regeneration. Due to the enormous potential of collagen-based regenerative barriers, clinicians may benefit from a review of potential applications of implantable collagen and knowledge of collagen preparation and membrane types as well as from as awareness of the functional and degradation properties of those materials.
Rates of different steps involved in the inhibition of protein synthesis by the toxic lectins abrin and ricin. (1976)

The kinetics of protein synthesis inhibition in a cell-free system from rabbit reticulocyte lysate was studied after addition of abrin and ricin and the isolated A chains. The toxin A chains inhibited protein synthesis at a rate proportional to the amount added. When intact toxins were added to the reticulocyte lysate, the kinetics of protein synthesis inhibition indicated that the A chains must be liberated before ribosome inactivation can take place. The splitting of the toxin in the lysate was directly demonstrated by the use of labeled toxins. The amount of abrin and ricin bound to HeLa cells under different experimental conditions was correlated to the concomitant inhibition of cellular protein synthesis. In the presence of lactose, which inhibits toxin binding, much higher concentrations of toxins were required to inhibit protein synthesis than in the absence of lactose. A linear relationship was found between the lactose concentration in the medium and the toxin concentration required to give 50% reduction in protein synthesis after 3 hours. The amount of toxin bound to the cell surfaces in the presence of lactose was either determined directly or calculated from the apparent association constant between toxins and surface receptors at the various lactose concentrations. Under different conditions involving a 300-fold variation in the concentration of toxin required to reduce protein synthesis by 50% after 3 hours, the amount of toxin bound to the cell surface was found to be the same. The toxicity thus appears to be determined by the number of toxin molecules bound to the cell surface. Purified ricin B chain was used to compete with the toxins for the receptor sites. Only after addition of high amounts of B chain was the toxicity of abrin and ricin appreciably reduced. The data do not support the view that receptors with especially high affinity are involved in the uptake of the toxins. When the time required for 50% inhibition was plotted versus the inverse value of the square root of the number of toxin molecules bound per cell, a straight line was obtained, intercepting at about 30 min. The data indicate that the observed lag time cannot be due entirely to the fact that the A chains must be liberated before they can act.