gebhart/patrepeval huggingface dataset:
Formation and Entrapment of Noble Metal Clusters in Silica Aerogel Monoliths by γ-Radiolysis (2002)

Noble metal clusters (Ag, Au) were formed in a silica aerogel matrix by γ-irradiation of hydrogel precursors loaded with aqueous solutions containing Ag+ or [AuCl4]- ions. Hydrogels exposed to γ-rays assumed the color expected for colloidal suspensions of Ag (respectively Au) clusters. The hydrogels were subsequently washed and supercritically dried, without any evident change in color, indicating that the metal clusters were not removed during drying. Typical γ-ray doses were between 2 and 3.5 kGy, and achieved complete reduction of hydrogels containing ion concentrations in the 10-4−10-3 M range. Reduction of solutions with higher metal ion concentrations was achieved by multiple irradiations. In the case of Ag+ reduction, we noticed that the radiolytic yield G was higher than that of the reducing radicals equal to G = 6 × 10-7 mol/L of reducing species per joule of absorbed energy. For example, a total dose of 3.5 kGy has led to a reduction of 5.7 × 10-2 M in a hydrogel with [Ag+] = 0.1 M. The high radiolytic yield has been attributed to radiation-induced oxide surface catalysis, or to autocatalysis, but the precise mechanism has not been determined. Metal clusters in the aerogel monoliths were characterized with optical absorption, transmission electron microscopy, X-ray diffraction, scanning electron microscopy, and X-ray photoelectron spectroscopy. These techniques have shown that the clusters have a crystalline fcc structure. Au clusters consist of pure Au, while surface oxidation of Ag clusters was observed with XPS.
Expression of the c-erbB-2 gene encoding a growth factor receptor. (1986)

The c-erbB-2 gene is a v-erbB-related proto-oncogene which encodes a protein similar to but distinct from the epidermal growth factor (EGF) receptor. In situ hybridization of metaphase spread showed that this gene is located on human chromosome 17 at q21, a specific breakpoint observed in a translocation associated with acute promyelocytic leukemia. The c-erbB-2 DNA probe hybridized with a 4.6 kb mRNA which directs the synthesis of a 185 kd glycoprotein. The 185 kd c-erbB-2 protein is associated with tyrosine kinase activity and is possibly phosphorylated by C-kinase on its serine and threonine residues. In addition, the c-erbB-2 protein is suggested to be a substrate for EGF receptor tyrosine kinase, since EGF binding to the EGF receptor rapidly induced phosphorylation of the c-erbB-2 protein on its tyrosine residue. Southern blot hybridization analysis of DNAs from human tumors demonstrated amplification of the c-erbB-2 gene restricted to adenocarcinomas. Finally, the promoter of the c-erbB-2 gene contains the typical TATA box and CAAT box as well as a GC box-like sequence. This is in contrast with the promoter of the EGF receptor gene, since the latter contains only GC-boxes. Therefore, it is suggested that the two genes are under different transcriptional control.
Morphinan alkaloids labeled with tritium: synthesis and applications (2013)

This review discusses the synthetic methods employed to tritiate morphinan alkaloids as well as their applications.
Movement of the tongue during normal breathing in awake healthy humans (2008)

Electromyographic (EMG) activity of the airway muscles suggest that genioglossus is the primary upper airway dilator muscle. However, EMG data do not necessarily translate into tissue motion and most imaging modalities are limited to assessment of the surfaces of the upper airway. In this study, we hypothesized that genioglossus moves rhythmically during the respiratory cycle and that the motion within is inhomogeneous. A 'tagged' magnetic resonance imaging technique was used to characterize respiratory-related tissue motions around the human upper airway in quiet breathing. Motion of airway tissues at different segments of the eupnoeic respiratory cycle was imaged in six adult subjects by triggering the scanner at the end of inspiration. Displacements of the 'tags' were analysed using the harmonic phase method (HARP). Respiratory timing was monitored by a band around the upper abdomen. The genioglossus moved during the respiratory cycle. During expiration, the genioglossus moved posteriorly and during inspiration, it moved anteriorly. The degree of motion varied between subjects. The maximal anteroposterior movement of a point tracked on the genioglossus was 1.02 +/- 0.54 mm (mean +/- s.d.). The genioglossus moved over the geniohyoid muscle, with minimal movement in other muscles surrounding the airway at the level of the soft palate. Local deformation of the tongue was analysed using two-dimensional strain maps. Across the respiratory cycle, positive strains within genioglossus reached peaks of 17.5 +/- 9.3% and negative strains reached peaks of -16.3 +/- 9.3% relative to end inspiration. The patterns of strains were consistent with elongation and compression within a constant volume structure. Hence, these data suggest that even during respiration, the tongue behaves as a muscular hydrostat.
Significantly accelerated osteoblast cell growth on aligned TiO2 nanotubes (2006)

Vertically aligned yet laterally spaced nanoscale TiO2 nanotubes have been grown on Ti by anodization, and the growth of MC3T3-E1 osteoblast cells on such nanotubes has been investigated. The adhesion/propagation of the osteoblast is substantially improved by the topography of the TiO2 nanotubes with the filopodia of growing cells actually going into the nanotube pores, producing an interlocked cell structure. The presence of the nanotube structure induced a significant acceleration in the growth rate of osteoblast cells by as much as approximately 300-400%.
IL-7 promotes thymocyte proliferation and maintains immunocompetent thymocytes bearing alpha beta or gamma delta T-cell receptors in vitro: synergism with IL-2. (1989)

IL-7 induced the proliferation of normal thymocytes and the effect was synergistically potentiated by a small dose of IL-2, which by itself hardly affected thymocyte proliferation. No synergism was observed between IL-7 and any one of the other lymphokines including IL-1, IL-3, and IL-4. The thymocyte culture stimulated with IL-7 and IL-2 consisted of single positive (CD4+CD8- and CD4-CD8+) and double negative (CD4-CD8-) populations, and double positive (CD4+CD8+) cells were completely deleted. Both single positive and double negative thymocytes expressed CD3, but only the former exhibited V beta 8 and V beta 6 in an expected proportion (approximately 30% in BALB/c mice) and the latter none at all. Immunoprecipitation of the cultured thymocytes by anti-TCR gamma antibody, on the other hand, revealed the presence of a TCR gamma chain. Taken together, these results indicated that the thymocyte cultured with IL-7 and IL-2 consisted of mature T cells bearing alpha beta or gamma delta TCR. Experiments using preselected thymocyte subpopulations indicated that double negative cells responded to both IL-7 and IL-2 with positive synergism when combined, while thymocytes enriched for single positive cells preferentially responded to IL-7 with little response to IL-2 and no detectable synergism. Double positive thymocytes showed no proliferation in response to IL-7 and IL-2. In contrast to single positive thymocytes, splenic T cells hardly responded to IL-7, although significant proliferation was induced in the presence of a low dose of IL-2. Thymocytes cultured with IL-7 and IL-2 showed little nonspecific cytotoxic activity, but responded to Con A or alloantigen, whereas those stimulated with a high dose of IL-2 alone exhibited potent cytotoxic activity. These results indicated that IL-7 was involved in the generation of immunocompetent T cells in the thymus in concert with IL-2.
A Purpose-Synthesised Anti-Fibrotic Agent Attenuates Experimental Kidney Diseases in the Rat (2012)

Together these studies suggest that broadly antagonising growth factor actions, including those of TGF-β1 and PDGF-BB, has the potential to protect the kidney from progressive injury in both the diabetic and non-diabetic settings.
Interleukin-13 Expression in the Nasal Mucosa of Perennial Allergic Rhinitis (1995)

Allergic rhinitis is an IgE mediated atopic disease characterized by elevated levels of allergen specific IgE antibodies that play a central role in mediating allergic reactions. Interleukin 13 (IL-13) is a novel T-cell-derived cytokine that shares several functional properties with IL-4 and has been demonstrated to be capable of inducing IgE synthesis. The present study was designed to investigate the expression of IL-13 gene in the epithelial compartment of the nasal mucosa of patients with perennial allergic rhinitis (PAR) to house dust mite, comparing it with that in the nasal epithelial compartment of chronic infectious rhinitis (CIR) patients and normal volunteers (NV). We also investigated the IL-13 gene expression in the peripheral blood of PAR patients. Nasal scrapings were collected from the inferior turbinate of patients undergoing conchotomy surgery and from outpatients, and the mRNA expression of IL-13 was analyzed by the RT-PCR method. Peripheral blood mononuclear cells were isolated by density-gradient centrifugation and the expression of IL-13 gene was examined by the RT-PCR method. IL-13 expression at protein level and its cell source were analyzed by immunohistochemistry of inferior turbinate biopsies. The levels of total serum IgE and allergen-specific IgE antibodies in the serum were estimated by the PRIST and CAP RAST method, respectively. IL-13 gene expression was detected in the epithelial compartment of the nasal mucosa of 18/19 PAR patients but was undetected in normal volunteers and CIR patients.(ABSTRACT TRUNCATED AT 250 WORDS)
Rendering for an interactive 360° light field display (2007)

We describe a set of rendering techniques for an autostereoscopic light field display able to present interactive 3D graphics to multiple simultaneous viewers 360 degrees around the display. The display consists of a high-speed video projector, a spinning mirror covered by a holographic diffuser, and FPGA circuitry to decode specially rendered DVI video signals. The display uses a standard programmable graphics card to render over 5,000 images per second of interactive 3D graphics, projecting 360-degree views with 1.25 degree separation up to 20 updates per second. We describe the system's projection geometry and its calibration process, and we present a multiple-center-of-projection rendering technique for creating perspective-correct images from arbitrary viewpoints around the display. Our projection technique allows correct vertical perspective and parallax to be rendered for any height and distance when these parameters are known, and we demonstrate this effect with interactive raster graphics using a tracking system to measure the viewer's height and distance. We further apply our projection technique to the display of photographed light fields with accurate horizontal and vertical parallax. We conclude with a discussion of the display's visual accommodation performance and discuss techniques for displaying color imagery.
Protective Cellular Immunity Against Influenza VirusInduced by Plasmid Inoculation of Newborn Mice (1997)

Neonate organisms display an intrinsic disability to mount effective immune responses to infectious agents or conventional vaccines. Whereas low doses of antigens trigger a suboptimal response, higher doses are frequently associated with tolerance induction. We investigated the ability of a plasmid-expressing nucleoprotein of influenza virus to prime a specific cellular immune response when administered to newborn mice. We found that persistent exposure to antigen following plasmid inoculation of neonates leads to a vigorous priming of specific CTLs rather than tolerance induction. The CTLs were cross-reactive against multiple strains of type A influenza viruses and produced IFNgamma but no IL-4. The immunity triggered by plasmid inoculation of neonates was protective in terms of pulmonary virus clearance as well as survival rate following lethal challenge with influenza virus. Whereas the persistence of the plasmid at the site of injection was readily demonstrable in adult mice at 3 months after inoculation, mice immunized as newborns displayed no plasmid at 3 months and very little at 1 month after injection. Thus, DNA-based immunization of neonates may prove an effective and safe vaccination strategy for induction of cellular immunity against microbes that cause serious infectious diseases in the early period of life.
Mechanism of nicotine-induced pulmonary fibroblast transdifferentiation (2005)

We tested the hypothesis that in vitro nicotine exposure disrupts specific epithelial-mesenchymal paracrine signaling pathways and results in pulmonary interstitial lipofibroblast (LIF)-to-myofibroblast (MYF) transdifferentiation, resulting in altered pulmonary development and function. Studies were done to determine whether nicotine induces LIF-to-MYF transdifferentiation and to elucidate underlying molecular mechanism(s) involved and to determine whether nicotine-induced LIF-to-MYF transdifferentiation could be prevented by stimulating specific alveolar interstitial fibroblast lipogenic pathway. WI38 cells, a human embryonic pulmonary fibroblast cell line, were treated with nicotine with or without specific agonists of alveolar fibroblast lipogenic pathway, PTHrP, DBcAMP, or the potent PPARgamma stimulant rosiglitazone (RGZ) for 7 days. Expression of key lipogenic and myogenic markers was examined by RT-PCR, Western hybridization, and immunohistochemistry. The effect of nicotine on triglyceride uptake by WI38 cells and PTHrP binding to its receptor was also determined. Finally, the effect of transfecting WI38 cells with a PPARgamma expression vector on nicotine-induced LIF-to-MYF transdifferentiation was determined. Nicotine treatment resulted in significantly decreased expression of lipogenic and increased expression of myogenic markers in a dose-dependent manner, indicating nicotine-induced LIF-to-MYF transdifferentiation. This was accompanied by decreased PTHrP receptor binding to its receptor. The nicotine-induced LIF-to-MYF transdifferentiation was completely prevented by concomitant treatment with PTHrP, DBcAMP, RGZ, and by transiently overexpressing PPARgamma. Our data suggest nicotine induces alveolar LIF-to-MYF transdifferentiation through a mechanism involving downregulation of lipogenic PTHrP-mediated, cAMP-dependent PKA signaling pathway, which can be prevented using specific molecular targets. Potential therapeutic implications of these observations against in utero nicotine-induced lung injury remain to be tested.
Idiopathic Scoliosis in Families of Children With Congenital Scoliosis (2002)

Although most cases of congenital scoliosis are thought to be of sporadic etiology, it is not known whether other types of spinal deformity occur in families of individuals with this type of scoliosis. Children with congenital scoliosis were identified through a review of health records and radiographic report databases. Of 237 children with congenital scoliosis investigated, 49 (20.7%) reported a family history of spinal deformity. Detailed pedigrees were done, which showed a history of idiopathic scoliosis in 17.3% of the 237 families. This is a higher than expected rate of spinal deformity in families of children with congenital scoliosis, and the strong association of idiopathic scoliosis in families of children with congenital scoliosis has not been reported previously. Although this finding could be related to the chance occurrence of multiple genetic abnormalities or sporadic events in these families, it does raise the possibility that one genetic defect at least predisposes these families to having different types of spinal deformity develop.
Analysis of the clinical problems in parkinsonism and the complications of long‐term levodopa therapy (1979)

We evaluated the current status of 131 patients with idiopathic parkinsonism who were receiving levodopa therapy. The residual parkinsonian symptoms and signs were tabulated, as were the adverse effects from medication. Response to therapy was correlated with duration of the disease and with duration of treatment. Patients with on-off or wearing-off effects were likely to have been treated for 4 years or longer. Patients treated with levodopa for 4 to 8 years were significantly more impaired with parkinsonism than patients treated for 0 to 3 years, even when patients were matched for total duration of disease. These data suggest that the deterioration of responsiveness after several years of levodopa therapy may be due to the therapy itself. Our findings support the concept that utilization of levodopa therapy should be delayed until a patient becomes significantly impaired in occupational or social situations.
Role of Brain-Derived Neurotrophic Factor in Target Invasion in the Gustatory System (1999)

Brain-derived neurotrophic factor (BDNF) is a survival factor for different classes of neurons, including gustatory neurons. We have studied innervation and development of the gustatory system in transgenic mice overexpressing BDNF under the control of regulatory sequences from the nestin gene, an intermediate filament gene expressed in precursor cells of the developing nervous system and muscle. In transgenic mice, the number and size of gustatory papillae were decreased, circumvallate papillae had a deranged morphology, and there was also a severe loss of lingual taste buds. Paradoxically, similar deficits have been found in BDNF knock-out mice, which lack gustatory neurons. However, the number of neurons in gustatory ganglia was increased in BDNF-overproducing mice. Although gustatory fibers reached the tongue in normal numbers, the amount and density of nerve fibers in gustatory papillae were reduced in transgenic mice compared with wild-type littermates. Gustatory fibers appeared stalled at the base of the tongue, a site of ectopic BDNF expression, where they formed abnormal branches and sprouts. Interestingly, palatal taste buds, which are innervated by gustatory neurons whose afferents do not traverse sites of ectopic BDNF expression, appeared unaffected. We suggest that lingual gustatory deficits in BDNF overexpressing mice are a consequence of the failure of their BDNF-dependent afferents to reach their targets because of the effects of ectopically expressed BDNF on fiber growth. Our findings suggest that mammalian taste buds and gustatory papillae require proper BDNF-dependent gustatory innervation for development and that the correct spatial expression of BDNF in the tongue epithelium is crucial for appropriate target invasion and innervation.
Morphology and mechanics of fungal mycelium (2017)

We study a unique biomaterial developed from fungal mycelium, the vegetative part and the root structure of fungi. Mycelium has a filamentous network structure with mechanics largely controlled by filament elasticity and branching, and network density. We report the morphological and mechanical characterization of mycelium through an integrated experimental and computational approach. The monotonic mechanical behavior of the mycelium is non-linear both in tension and compression. The material exhibits considerable strain hardening before rupture under tension, it mimics the open cell foam behavior under compression and exhibits hysteresis and the Mullins effect when subjected to cyclic loading. Based on our morphological characterization and experimental observations, we develop and validate a multiscale fiber network-based model for the mycelium which reproduces the tensile and compressive behavior of the material.
Metal Complexes with 2,3-Bis(diphenylphosphino)-1,4-diazadiene Ligands:  Synthesis, Structures, and an Intramolecular Metal-Mediated [4 + 2] Cycloaddition Employing a Benzene Ring as a Dienophile (2002)

2,3-Bis(diphenylphosphino)-1,4-diazadienes RN=C(PPh2)-C(PPh2)=NR (1a, R = 4-tolyl; 1b, R = 4-tert-butylphenyl; 1c, R = mesityl) were used as novel ligands for transition metals. The metal complexes [(1c)Mo(CO)4] (2a), [(1c)[Mo(CO)4]2] (2b), [(1a)Cu(Cl)(PPh3)] (3), and [(1b)[(NiBr2(THF))]2] (4) were characterized by elemental analysis, MS, and 31P[1H], 1H, and 13C NMR spectra (except the paramagnetic complex 4). Additionally, the molecular structure of the complexes in the solid state was determined by single-crystal X-ray diffraction. In 2a and 2b the chelating ligand coordinates via the N,P donor set, whereas in 3 the chelating ligand coordinates via the two P atoms. 4 contains a square-planar (P,P)NiBr2 moiety on the one side of the bridging ligand 1b. On the opposite side the 1,2-dimine unit bonds to another Ni center having octahedral geometry. The bulkier ligand 1c reacts to form the mononuclear compound 5. X-ray diffraction analysis of single crystals shows that 5 contains a quinoxaline derivative with a cyclohexa-1,3-diene ring in the peripheral position. Furthermore, it contains a bis(diphenylphosphino)-ethylene unit coordinating the NiBr2. This arrangement is the result of an intramolecular [4 + 2] cycloaddition between the 1,2-diimine unit (as diheterodiene) and the benzene ring of the 4-tolyl-N substituent (as dieneophile). The same type of ring-closing reaction followed by a tautomerization reaction to form the mononuclear compound 6 occurred by dissolution of the binuclear complex 4 in methanol. This reaction can be used as a simple method for the synthesis of novel 1,2-bis(diarylphosphanyl)ethylenes containing a quinoxaline backbone.
Laparoscopic Partial Nephrectomy: 3-Year Followup (2006)

At 3-year followup LPN provides oncological outcomes comparable to those in contemporary open partial nephrectomy series.
Gene transfer in vivo: sustained expression and regulation of genes introduced into the liver by receptor-targeted uptake. (1994)

Receptor-mediated gene transfer has been used to introduce genes into tissues of animals in vivo. The genes introduced by this approach have been transiently expressed at low levels in animal tissues. High levels of expression, for longer periods, have been attained by the induction of cell division (i.e., partial hepatectomy) or disruption of lysosomal degradation of the DNA. We have studied the correlation of specific structural features on the DNA/ligand complexes with their ability to efficiently introduce DNA into the livers of intact animals. A chimeric gene containing the phosphoenolpyruvate carboxykinase gene promoter (nucleotides -460 to +73) linked to the structural gene for human factor IX (PEPCK-hFIX gene) was condensed with galactosylated poly(L-lysine) by titration with NaCl, resulting in complexes of defined size (10-12 nm in diameter) and shape. The PEPCK-hFIX gene complex was injected into the caudal vena cava of adult rats and the conjugated DNA was specifically targeted to the livers of the animals; no detectable DNA was noted in other tissues. The plasmid containing the PEPCK-hFIX gene was found as an episome in the livers of the rats 32 days after injection of the DNA complex. Human factor IX DNA, mRNA, and functional protein were detected up to 140 days after administration of the DNA complex (the duration of the experiment). Transcription from the PEPCK promoter could be induced over the entire course of the experiment by feeding the rats a high-protein, carbohydrate-free diet. We conclude that the structure of the DNA/ligand complexes is of key importance for the successful introduction of genes into the tissues of animals by receptor-mediated endocytosis.
Corticotropin releasing factor receptor antagonists for major depressive disorder (2011)

CRF1 receptor antagonists are expected to be beneficial only for those patients with CRF overexpression and the need for tests to identify these individuals is discussed. New technical developments and diagnostic tools might eventually lead to a more successful treatment of major depression with CRF1 receptor antagonists.