gebhart/patrepeval huggingface dataset:
Optimization of Extraction Techniques and RP-HPLC Analysis of Antidiabetic and Anticancer Drug Mangiferin from Roots of ‘Saptarangi’ (Salacia chinensis L.) (2015)

Saptarangi (Salacia chinensis L.) is a chief source of mangiferin, which has antidiabetic and anticancer properties. Extraction using continuous shaking extraction (CSE), ultrasonic extraction (UE), microwave assisted extraction (MAE), and steam bath assisted extraction (SBAE) were evaluated for extraction of mangiferin (MF) from roots of S. chinensis. The extracts were analyzed by reverse phase-high performance liquid chromatography (RP-HPLC) and Fourier transform-infra red spectroscopy (FT-IR) analysis. Operational parameters such as different solvents and exposure period were also evaluated. N,N-Dimethyl formamide (DMF 30%, v/v) extracted higher quantity of MF compared to other solvents. The results shows that the extraction of MF by SBAE was more efficient followed by MAE, CSE, and UE methods. Maximum quantity of MF (391.80 ppm) was obtained by SBAE technique and DMF (30%) as a solvent system. SBAE is the easiest and best method for screening of MF from S. chinensis. Furthermore, use of FT-IR validates the RP-HPLC technique for identification and quantification.
A 92 nm Bandwidth Raman Amplifier (1998)

Over the last several years, much attention has been paid to the development of broad bandwidth erbium doped fiber amplifiers for future dense, high capacity wavelength division multiplexed systems [1].
Fiber-optic probe for in vivo measurement of oxygen partial pressure (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFiber-optic probe for in vivo measurement of oxygen partial pressureJohn I. Peterson, Raphael V. Fitzgerald, and Delwin K. BuckholdCite this: Anal. Chem. 1984, 56, 1, 62–67Publication Date (Print):January 1, 1984Publication History Published online1 May 2002Published inissue 1 January 1984https://pubs.acs.org/doi/10.1021/ac00265a017https://doi.org/10.1021/ac00265a017research-articleACS PublicationsRequest reuse permissionsArticle Views1055Altmetric-Citations235LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Neurogenesis in Adult Human (1996)

THIS report describes neurogenesis in the adult human olfactory epithelium in vitro. Olfactory epithelium was collected at autopsy and by biopsy, and grown in serum-free medium. Basic fibroblast growth factor induced the differentiation of bipolar cells which were immunopositive for several neuronal proteins but not glial proteins. [3H]thymidine autoradiography confirmed that these neurones were born in vitro. The results demonstrate that the adult human olfactory epithelium retains the capacity for neurogenesis and neuronal differentiation, at least until the age of 72 years. It is now possible to examine neurones and neurogenesis in biopsies from patients with disorders that may involve a neurodevelopmental or neurodegenerative aetiology such as schizophrenia, bipolar disorder and Alzheimer's disease.
Effects of cortisol on lipolysis and regional interstitial glycerol levels in humans (2002)

Cortisol's effects on lipid metabolism are controversial and may involve stimulation of both lipolysis and lipogenesis. This study was undertaken to define the role of physiological hypercortisolemia on systemic and regional lipolysis in humans. We investigated seven healthy young male volunteers after an overnight fast on two occasions by means of microdialysis and palmitate turnover in a placebo-controlled manner with a pancreatic pituitary clamp involving inhibition with somatostatin and substitution of growth hormone, glucagon, and insulin at basal levels. Hydrocortisone infusion increased circulating concentrations of cortisol (888 +/- 12 vs. 245 +/- 7 nmol/l). Interstitial glycerol concentrations rose in parallel in abdominal (327 +/- 35 vs. 156 +/- 30 micromol/l; P = 0.05) and femoral (178 +/- 28 vs. 91 +/- 22 micromol/l; P = 0.02) adipose tissue. Systemic [(3)H]palmitate turnover increased (165 +/- 17 vs. 92 +/- 24 micromol/min; P = 0.01). Levels of insulin, glucagon, and growth hormone were comparable. In conclusion, the present study unmistakably shows that cortisol in physiological concentrations is a potent stimulus of lipolysis and that this effect prevails equally in both femoral and abdominal adipose tissue.
Osteopontin--a possible anchor of osteoclasts to bone. (1990)

A key event in bone resorption is the binding of osteoclasts to the mineral matrix of bone surfaces. A candidate for mediating this binding is osteopontin, a major cell- and hydroxyapatite-binding protein synthesized by osteoblasts. In support of this hypothesis is the fact that the synthesis of osteopontin is stimulated by calcitriol (1,25-dihydroxy-vitamin(D3), a substance that induces bone resorption. The present study demonstrates that osteopontin is highly enriched at regions of the bone surface where osteoclasts are anchored. Furthermore, the vitronectin receptor, which has known specificity for osteopontin, is shown preferentially localized at the corresponding area of the osteoclast plasma membrane. The results thus support the hypothesis that osteoclasts when resorbing bone are anchored by osteopontin bound both to the mineral of bone matrix and to a vitronectin receptor on the osteoclast plasma membrane.
Effective renaturation of denatured and reduced immunoglobulin G in vitro without assistance of chaperone (1996)

IgG is an oligomeric protein that consists of two heavy and two light chains. To form the oligomer, a highly concentrated protein would be required on renaturation. On the other hand, refolding of proteins at high concentration often led to aggregation. Therefore, denatured and reduced oligomeric protein scarcely refolded to the native structure. As was expected, the folding yield of the denatured and reduced IgG was below 5% under the condition employed in rapid dilution. The low folding yield was elucidated to be due to assembly or aggregation. Using a renaturation method previously developed to depress aggregation effectively by means of slow dialysis, the refolding yield of the denatured and reduced IgG at above 1 mg/ml was above 70%. Most of the refolded IgG was identical with the intact material based on analyses by affinity chromatography and SDS-PAGE.
Positive regulation of the Escherichia coli glycine cleavage enzyme system (1993)

A new mutation in Escherichia coli, designated gcvA1, that results in noninducible expression of both gcv and a gcvT-lacZ gene fusion was isolated. A plasmid carrying the wild-type gcvA gene complemented the mutation and restored glycine-inducible gcv and gcvT-lacZ gene expression. These results suggest that gcvA encodes a positive-acting regulatory protein that acts in trans to increase expression of gcv.
Exploration and Customization of FPGA-Based Soft Processors (2007)

As embedded systems designers increasingly use field-programmable gate arrays (FPGAs) while pursuing single-chip designs, they are motivated to have their designs also include soft processors, processors built using FPGA programmable logic. In this paper, we provide: 1) an exploration of the microarchitectural tradeoffs for soft processors and 2) a set of customization techniques that capitalizes on these tradeoffs to improve the efficiency of soft processors for specific applications. Using our infrastructure for automatically generating soft-processor implementations (which span a large area/speed design space while remaining competitive with Altera's Nios II variations), we quantify tradeoffs within soft-processor microarchitecture and explore the impact of tuning the microarchitecture to the application. In addition, we apply a technique of subsetting the instruction set to use only the portion utilized by the application. Through these two techniques, we can improve the performance-per-area of a soft processor for a specific application by an average of 25%
Purification and Characterization of Thiol-Specific Antioxidant Protein from Human Liver: A Mer5-Like Human Isoenzyme (1996)

A 23-kDa molecular mass of antioxidant protein was purified from human liver. This protein exhibited the preventive effect against the inactivation of glutamine synthetase by a metal-catalyzed oxidation system. This antioxidant activity was supported by a thiol-reducing equivalent such as dithiothreitol in a similar manner to that of the 25-kDa thiol-specific antioxidant protein (TSA) from human red blood cells (HR). However, a thioredoxin-linked peroxidase activity of thiol-specific antioxidant protein of human liver (HLTSA) (0.91 of HLTSA) was much lower than that of thiol-specific antioxidant protein of human red blood cells (HRTSA) (16.4 of HRTSA). This HLTSA is also immnologically distinct from HRTSA Amino acid sequences of the three tryptic peptides (P1, P2, P3) of HLTSA were found to be completely homologous to segments of the known Mer5-like protein, which belongs to the known TSA family.
Pathophysiological Roles of Calreticulin in Autoimmune Disease (1999)

Autoantibodies against the endoplasmic reticulum (ER) luminal protein, calreticulin are often present in sera from patients with systemic lupus erythematosus, rheumatic disease and various parasitic diseases including onchocerciasis. New information has revealed that calreticulin is implicated in a number of autoimmune processes, including molecular mimicry, epitope spreading, complement inactivation and stimulation of inflammatory mediators, such as nitric oxide production. Calreticulin also binds to the Ro/SS-A antigen complex, which is composed of at least three immunologically distinct proteins bound to a group of small cytoplasmic RNAs that together form a common target for autoimmune responses. Up-regulation of calreticulin at the protein and RNA levels can be triggered by cell stresses, including heat shock, exposure to heavy metals and perturbation of normal ER function, which may in some cases lead to its secretion from cells. Calreticulin is targeted by autoantibodies following its release into the extracellular environment, possibly as a result of cell death, or its presence at the cell surface in response to insults such as viral infection or ultraviolet irradiation. These findings suggest that calreticulin is not just an autoantigen, but plays an active role in the pathology of various autoimmune disease through determinant spreading.
Using XML to Build Consistency Rules for Distributed Specifications (2000)

The work presented in this paper is part of a large programme of research aimed at supporting consistency management of distributed documents on the World Wide Web. We describe an approach for specifying consistency rules for distributed partial specifications with overlapping contents. The approach is based on expressing consistency rules using XML and XPointer. We present a classification for different types of consistency rules, related to various types of inconsistencies and show how to express these consistency rules using our approach. We also briefly describe a rule editor to support the specification of the consistency rules.
Phase I/II study of vaccination with dendritic‐like leukaemia cells for the immunotherapy of acute myeloid leukaemia (2006)

Summary Twenty‐two patients with acute myeloid leukaemia were recruited into a phase I/II clinical trial investigating the vaccination of patients in complete remission (CR) with autologous dendritic‐like leukaemia cells (DLLC). At trial entry, leukaemia cells were harvested and tested for their ability to undergo cytokine‐induced dendritic cell differentiation. Patients were then treated with intensive chemotherapy. Five patients achieved both CR and had leukaemia cells that successfully underwent differentiation and therefore proceeded to vaccination. Four escalating doses of DLLC were administered weekly by subcutaneous injection. Vaccination was generally well tolerated although one patient developed extensive eczema and an increased antinuclear factor titre possibly indicating induction of autoimmunity. Development of anti‐leukaemic T‐cell responses was assessed by enzyme‐linked immunospot analysis of gamma‐interferon secreting T lymphocytes and by human leucocyte antigen tetramer analysis for WT1‐specific T cells. Increases in anti‐leukaemic T‐cell responses were demonstrated in four patients, but only two of the five remained in remission more than 12 months postvaccination. The study has demonstrated that generation of DLLC is feasible in only a subgroup of patients and is currently neither broadly applicable or clinically effective.
Measuring pay-per-install: the commoditization of malware distribution (2011)

Recent years have seen extensive diversification of the underground economy associated with malware and the subversion of Internet-connected systems. This trend towards specialization has compelling forces driving it: miscreants readily apprehend that tackling the entire value-chain from malware creation to monetization in the presence of ever-evolving countermeasures poses a daunting task requiring highly developed skills and resources. As a result, entrepreneurial-minded miscreants have formed pay-per-install (PPI) services--specialized organizations that focus on the infection of victims' systems.

In this work we perform a measurement study of the PPI market by infiltrating four PPI services. We develop infrastructure that enables us to interact with PPI services and gather and classify the resulting malware executables distributed by the services. Using our infrastructure, we harvested over a million client executables using vantage points spread across 15 countries. We find that of the world's top 20 most prevalent families of malware, 12 employ PPI services to buy infections. In addition we analyze the targeting of specific countries by PPI clients, the repacking of executables to evade detection, and the duration of malware distribution.
Spatial Fluctuations and Localisation Effects in Optical Characteristics of p-Doped GaN Films (2001)

We report the observation of several intense satellite lines in the emission of p-GaN excited at 325 nm with separations suggesting a multiple LO phonon-related process. The observed phenomenon is interpreted as hot exciton luminescence enhanced by potential fluctuations and localisation. Using optically detected magnetic resonance and photoluminescence kinetics we explore the compensation processes underpinning these localisation effects.
Insights into glutamate transport regulation in human astrocytes: Cloning of the promoter for excitatory amino acid transporter 2 (EAAT2) (2003)

Glutamate transport is central to neurotransmitter functions in the brain. Impaired glutamate transport induces neurotoxicity associated with numerous pathological processes, including stroke/ischemia, temporal lobe epilepsy, Alzheimer's disease, amyotrophic lateral sclerosis, Huntington's disease, HIV-1-associated dementia, and growth of malignant gliomas. Excitatory amino acid transporter-2 (EAAT2) is a major glutamate transporter in the brain expressed primarily in astrocytes. We presently describe the cloning and characterization of the human EAAT2 promoter, demonstrating elevated expression in astrocytes. Regulators of EAAT2 transport, both positive and negative, alter EAAT2 transcription, promoter activity, mRNA, and protein. These findings imply that transcriptional processes can regulate EAAT2 expression. Moreover, they raise the intriguing possibility that the EAAT2 promoter may be useful for targeting gene expression in the brain and for identifying molecules capable of modulating glutamate transport that could potentially inhibit, ameliorate, or prevent various neurodegenerative diseases.
The Activation of Prothrombin (1973)

Abstract The intermediates formed during the activation of prothrombin in 25% sodium citrate solutions have been isolated and partially characterized. Their properties are in agreement with those predicted by a previously proposed model for prothrombin activation in 25% sodium citrate: prothrombin (72,000 daltons) → intermediate 1 (65,000 daltons) → Intermediate 2 (39,000 daltons) and Intermediate 3 (25,000 daltons); Intermediate 2 (39,000 daltons) → thrombin (α-thrombin, 39,000 daltons) (Mann, K. G., Heldebrant, C. M., and Fass, D. N. (1971) J. Biol Chem. 246, 6106–6114). Intermediates 1 and 2, as predicted, can be activated to thrombin, while Intermediate 3 cannot. The chromatographic behavior of Intermediate 2 is nearly identical with that of thrombin. Molecular weight and amino acid composition data strongly suggest that α-thrombin is formed from Intermediate 2 by the loss of little, if any, of the Intermediate 2 sequence. Intermediates 2 and 3 are shown to be substantially independent portions of the prothrombin sequence. The tryptic peptides derived from Intermediates 2 and 3, while very dissimilar account for greater than 85% of the tryptic peptides derived from prothrombin. Furthermore, Intermediate 3 contains nearly twice as much half-cystine and more than twice as much neutral sugar as Intermediate 2. A product of prothrombin activation, apparently derived from Intermediate 3, has been shown to alter the activity of thrombin toward N-α-tosyl-l-arginine methyl ester and fibrinogen. The possibility that a thrombin of altered specificity may participate in the control and regulation of coagulation is discussed.
Current prospects for the treatment of clinical sepsis. (1994)

Clinical trials of antiendotoxin antibodies have not shown them to have therapeutic benefit. New agents that neutralize or antagonize the cellular effects of endotoxin may provide an alternative means to inhibit endotoxin effects during severe Gram-negative infections. Anti-interleukin-1 and antitumor necrosis factor-alpha therapies have demonstrated efficacy in animal models, but the results have been inconsistent in human trials. Preliminary results from clinical trials of cytokine antagonists suggest that these therapies may be effective in the most severely ill patients. Further clinical trials will be required to determine the therapeutic role of these agents in septic shock.
Laser-frequency mixing in the junction of a scanning tunneling microscope (1987)

The frequency response and the frequency-mixing properties of the tunneling junction of a scanning tunneling microscope (STM) were investigated using the radiation of two CO2 lasers. Difference frequencies of up to 90 MHz were detected over a wide range of bias voltages and tunneling currents. At tunneling currents typical of STM operation the beat signal decreases as the bias voltage is increased. However, this dependence is reversed at higher tunneling currents, where the STM begins to resemble a point-contact metal-insulator-metal diode. For low tunneling currents the generation of the beat signal is attributed mainly to thermally assisted tunneling.
Studies on Polynucleotides. I. A New and General Method for the Chemical Synthesis of the C5″-C3″ Internucleotidic Linkage. Syntheses of Deoxyribo-dinucleotides1 (1958)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies on Polynucleotides. I. A New and General Method for the Chemical Synthesis of the C5″-C3″ Internucleotidic Linkage. Syntheses of Deoxyribo-dinucleotides1P. T. Gilham and H. G. KhoranaCite this: J. Am. Chem. Soc. 1958, 80, 23, 6212–6222Publication Date (Print):December 1, 1958Publication History Published online1 May 2002Published inissue 1 December 1958https://pubs.acs.org/doi/10.1021/ja01556a016https://doi.org/10.1021/ja01556a016research-articleACS PublicationsRequest reuse permissionsArticle Views1089Altmetric-Citations162LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Teduglutide, a novel glucagon-like peptide 2 analog, in the treatment of patients with short bowel syndrome (2012)

Short bowel syndrome results from surgical resection, congenital defect or disease-associated loss of absorption. Parenteral support (PS) is lifesaving in patients with short bowel syndrome and intestinal failure who are unable to compensate for their malabsorption by metabolic or pharmacologic adaptation. Together, the symptoms of short bowel syndrome and the inconvenience and complications in relation to PS (e.g. catheter-related blood steam infections, central thrombosis and intestinal failure associated liver disease) may impair the quality of life of patients. The aim of treatment is to maximize intestinal absorption, minimize the inconvenience of diarrhea, and avoid, reduce or eliminate the need for PS to achieve the best possible quality of life for the patient. Conventional treatments include dietary manipulations, oral rehydration solutions, and antidiarrheal and antisecretory treatments. However, the evidence base for these interventions is limited and treatments that improve the structural and functional integrity of the remaining intestine are needed. Teduglutide, an analog of glucagon-like peptide 2, improves intestinal rehabilitation by promoting mucosal growth and possibly by restoring gastric emptying and secretion, thereby reducing intestinal losses and promoting intestinal absorption. In a 3-week, phase II balance study, teduglutide reduced diarrhea by around 700 g/day and fecal energy losses by around 0.8 MJ/day. In two randomized, placebo-controlled, 24-week, phase III studies, similar findings were obtained when evaluating the fluid composite effect, which is the sum of the beneficial effects of teduglutide - reduction in the need for PS, increase in urine production and reduction in oral fluid intake. The fluid composite effect reflects the increase in intestinal fluid absorption (and the concomitant reduction in diarrhea) and may be used in studies in which metabolic balance assessments are not performed. In studies of up to 24 weeks' duration, teduglutide appears to be safe and well tolerated. Treatment with teduglutide was associated with enhancement or restoration of the structural and functional integrity of the remaining intestine with significant intestinotrophic and proabsorptive effects, facilitating a reduction in diarrhea and an equivalent reduction in the need for PS in patients with short bowel syndrome and intestinal failure.