gebhart/patrepeval huggingface dataset:
Mechanical Connections Between Elastin and Collagen (1994)

The ligament supporting the leading edge of birds' wings is a connective tissue structure with unusual morphologic and elastic features. Its center section is made of a highly extensible composite of elastin and collagen fibers and its two end sections of nearly inextensible pure collagen; these are joined end-to-end in short interdigitating junctions. Substantial forces are transmitted through the junctions showing that collagen and elastin are mechanically connected. The junctions and elastic segment are sufficiently strong that when the intact ligament is maximally strained, the point of failure is commonly in the collagenous segments or their attachments to the tissues of origin or insertion. Here we outline the morphology and describe static force-length properties of this ligament.
Effects of EGF, FGF, and PDGF on Canine Chondrocytes in Three-Dimensional Culture (1997)

Summary Three-dimensional (3-D) culture of chondrocytes has been shown to promote cell proliferation, differentiation, and production of matrix while providing for cell morphology and matrix characteristics that resemble normal articular cartilage (1-3). In a recently published study, enhanced glycosaminoglycan (GAG) production was demonstrated in canine chondrocytes in 3-D culture on cancellous bone substrate (3). The effects of growth factors known to be present in cryopreserved bone were suggested to be responsible. Various growth factors including epidermal growth factor (EGF), fibroblast growth factor (FGF), and plateletderived growth factor (PDGF) have been shown to enhance chondrocyte DNA production, mitotic activity, and matrix production (5-10). The objective of the study reported here was to evaluate the effects of one concentration of EGF, FGF, and PDGF on canine chondrocyte proliferation and production of normal matrix constituents in 3-D culture. Canine articular chondrocytes were cultured in three-dimensional medium for 25 days. EGF, FGF, and PDGF were added to the culture medium. Chondrocytes in 3-D culture maintained viability and differentiation. Cell morphology and matrix production resembled that of intact hyaline cartilage. Significant differences with respect to cell counts, glycosaminoglycan concentration, or collagen type II immuno-reactivity as the result of added growth factors were not found.
CD28 ligation in T-cell activation: evidence for two signal transduction pathways (1990)

The CD28 homodimer is thought to function as a signal transducing receptor during activation of T cells. Evidence is presented that the degree of aggregation of CD28 on the cell surface regulates two distinct CD28-associated signals. Binding of bivalent CD28 monoclonal antibody (MoAb) 9.3 upregulates lymphokine production by messenger RNA (mRNA) stabilization, without direct initiation of lymphokine mRNA transcription. This signal was not dependent on inositol phospholipid production or activation of a protein tyrosine kinase (PTK). In contrast, further crosslinking of CD28 on the cell surface rapidly induced formation of large amounts of inositol trisphosphate (InsP3) and increased cytoplasmic calcium concentration [( Ca2+]i), but did not stimulate PTK. CD28 crosslinking directly activated a subset of resting T cells, since CD25 (interleukin [IL]-2 receptor alpha chain) mRNA was rapidly induced in purified T cells, and proliferation, even without addition of exogenous IL-2, was sometimes observed. CD25 expression was detected on the cell surface of approximately 20% of CD4+ T cells. The degree of CD28 aggregation required for activation was investigated by preparing soluble 9.3 x 9.3 conjugates ranging in size from approximately 300 Kd to greater than 1,000 Kd, and comparing their function in T-cell proliferation assays with phorbol-12-myristate-13- acetate (PMA), anti-CD3, or IL-2. There was a correlation between conjugate size and proliferation with IL-2, whereas costimulation with PMA or CD3 was optimized at a lower degree of CD28 aggregation. The inositol phospholipid (InsP) generation and increase in [Ca2+]i after CD28 receptor aggregation appeared to proceed through a pathway different from the CD3/T-cell receptor (TCR) pathway since it was enhanced by pretreatment with PMA, while the InsP and [Ca2+]i signal from crosslinking CD3 was suppressed by PMA. Furthermore, the proliferation response to CD28 aggregation was resistant to inhibition by CD3 modulation. Thus, CD28 aggregation appears to trigger a phospholipase C activation pathway that differs from the CD3/TCR-linked pathway.
The Obtaining of Cobalt Multilayers by Programme-controlled Pulse Current (1997)

SummaryThis paper deals with obtaining cobalt films by means of the programme-controlled pulsed current electrolysis method. A method of depositing multilayered cobalt films composed of alternating layers of h.c.p. and f.c.c. phases is suggested. An X-ray analysis of the cobalt films phase compositions has been carried out.
Profile-Based Focused Crawler for Social Media-Sharing Websites (2008)

In this paper, we present a novel profile based focused crawling system for dealing with increasingly popular social media-sharing Web sites. In this system, we treat users' profiles as ranking criteria for guiding the crawling process. Furthermore, we divide a user's profile into two parts, an internal part, which comes from the user's own contribution, and an external part, which comes from the user's social contacts. In order to efficiently and effectively extract data from a social media-sharing website for focused crawling, a path string based page-classification method was first developed for identifying list pages, detail pages and profile pages.
The mitochondrial transporter SLC25A43 is frequently deleted and may influence cell proliferation in HER2-positive breast tumors (2012)

We have found deletion in the SLC25A43 gene to be a common event in HER2-positive breast cancer as well as in other cancers. In addition, the SLC25A43 protein expression was shown to be related to S-phase fraction in HER2-positive breast cancer. Our results indicate a possible role of SLC25A43 in HER2-positive breast cancer and support the hypothesis of altered mitochondrial function in cancer.
Semantic ranking and result visualization for life sciences publications (2010)

An ever-increasing amount of data and semantic knowledge in the domain of life sciences is bringing about new data management challenges. In this paper we focus on adding the semantic dimension to literature search, a central task in scientific research. We focus our attention on PubMed, the most significant bibliographic source in life sciences, and explore ways to use high-quality semantic annotations from the MeSH vocabulary to rank search results. We start by developing several families of ranking functions that relate a search query to a document's annotations. We then propose an efficient adaptive ranking mechanism for each of the families. We also describe a two-dimensional Skyline-based visualization that can be used in conjunction with the ranking to further improve the user's interaction with the system, and demonstrate how such Skylines can be computed adaptively and efficiently. Finally, we evaluate the effectiveness of our ranking with a user study.
Validity of the single processor approach to achieving large scale computing capabilities (1967)

For over a decade prophets have voiced the contention that the organization of a single computer has reached its limits and that truly significant advances can be made only by interconnection of a multiplicity of computers in such a manner as to permit cooperative solution. Variously the proper direction has been pointed out as general purpose computers with a generalized interconnection of memories, or as specialized computers with geometrically related memory interconnections and controlled by one or more instruction streams.
Mechanisms and future directions for prevention of vein graft failure in coronary bypass surgery (2002)

Coronary artery bypass grafting has been utilized as a beneficial treatment for myocardial ischemic disease for over three decades. Failure of coronary artery venous grafts occurs at a substantial rate and has a large impact on two main endpoints, survival and quality of life. An exhaustive amount of basic research has been generated to delineate possible mechanisms responsible for graft failure and modalities to prevent its occurrence. Although pharmacotherapy exists, insufficient translation has emerged from the bench to the operating room. In this article, we review the literature regarding the current mechanisms and mediators including growth factors, nitric oxide and genetics leading to saphenous vein graft occlusion. The review addresses the current state of affairs and modes for prevention of vein graft failure perioperatively and newer technologies that may help ameliorate this problem in the future.
Inhibitory effects of soy and rye diets on the development of Dunning R3327 prostate adenocarcinoma in rats (1998)

METHODS Dunning R3327 PAP prostate tumors were transplanted in 125 rats, the rats were divided into five groups, and tumor development was examined for 24 weeks during treatment with diets containing 33% of soy flour (SD), rye bran (RB), heat-treated rye bran (HRB), or rye endosperm (RE). RESULTS In the SD, RB, and HRB groups, significantly fewer palpable tumors and lower tumor volume were detected 14 and 16 weeks after transplantation when compared with the control, fiber-free dietary (FF) group. The body weight was lower 16 weeks after tumor transplantation in the RB and HRB groups when compared with the control group (P < 0.05). Rats in the RB and the HRB groups had a significant lower energy intake than the FF group during the first metabolic observation period, 3–6 weeks after tumor transplantation (P < 0.05), whereas the energy intake was the same in all groups during the second metabolic observation period, 13–16 weeks after tumor transplantation. However, when the tumor volume was adjusted for the body weight of the animals, there were still significant lower tumor volumes in the SD, RB, and HRB groups compared with the FF group (P < 0.05). A significant increase in daily urinary excretion of the isoflavonoids, daidzein, O-desmethylangolensin, equol, and Genistein, was observed in the rats fed SD, and of the ligands enterolactone and enterodiol in the rats fed RB and HRB during both metabolic periods. There were no differences in testosterone levels between the groups. CONCLUSIONS The present study shows that SD inhibits implanted prostate cancer growth. Although RB and HRB had a protective effect, further studies are needed to exclude the possibility that a low energy intake played a role in this respect. The results suggest that phytoestrogens (isoflavonoids and ligands), may be responsible for the delayed prostate tumor growth. Prostate 36:151–161, 1998. © 1998 Wiley-Liss, Inc.
NMR relaxation times T1 and T2 of water in plasma from patients with lung carcinoma: Correlation of T2 with blood sedimentation rate (1987)

The T1 and T2 NMR relaxation times of water protons in plasma, obtained from patients with lung carcinoma, healthy volunteers, and patients with nontumor diseases were measured at a resonance frequency of 20 MHz. Additionally hematologic parameters were determined. In tumor patients the mean plasma T2 was shortened by about 20%, and an increase in the blood sedimentation rate (BSR) was noted. Similar but less pronounced results were found for the nontumor group of patients, indicating that the shortening of T2 in plasma is a secondary host response. However, a plot of plasma 1/T2 versus BSR from tumor patients showed a significant correlation between these two parameters. No such correlation could be detected in the nontumor group of patients. The correlation of 1/T2 with BSR, found solely in the tumor patient group, increased the diagnostic sensitivity of T2 measurements and may help to differentiate between malignant and nonmalignant disease. No significant variation in the T1 spin-lattice relaxation time was observed.
Photoinitiated Electron Collection in a Mixed-Metal Trimetallic Complex of the Form {[(bpy)2Ru(dpb)]2IrCl2}(PF6)5 (bpy = 2,2'-Bipyridine and dpb = 2,3-Bis(2-pyridyl)benzoquinoxaline) (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotoinitiated Electron Collection in a Mixed-Metal Trimetallic Complex of the Form {[(bpy)2Ru(dpb)]2IrCl2}(PF6)5 (bpy = 2,2'-Bipyridine and dpb = 2,3-Bis(2-pyridyl)benzoquinoxaline)Sharon M. Molnar, Girlie Nallas, Jon S. Bridgewater, and Karen J. BrewerCite this: J. Am. Chem. Soc. 1994, 116, 12, 5206–5210Publication Date (Print):June 1, 1994Publication History Published online1 May 2002Published inissue 1 June 1994https://pubs.acs.org/doi/10.1021/ja00091a026https://doi.org/10.1021/ja00091a026research-articleACS PublicationsRequest reuse permissionsArticle Views575Altmetric-Citations132LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Monoclonal Antibodies to the Rough Lipopolysaccharide of Neisseria meningitidis Protect Infant Rats From Meningococcal Infection (1988)

Journal Article Monoclonal Antibodies to the Rough Lipopolysaccharide of Neisseria meningitidis Protect Infant Rats From Meningococcal Infection Get access Kirsi Saukkonen, Kirsi Saukkonen Please address requests for reprints to Dr. Kirsi Saukkonen, Box 152, Rockefeller University, 1230 York Avenue, New York, New York 10021. Search for other works by this author on: Oxford Academic PubMed Google Scholar Maija Leinonen, Maija Leinonen Search for other works by this author on: Oxford Academic PubMed Google Scholar Helena Käyhty, Helena Käyhty Search for other works by this author on: Oxford Academic PubMed Google Scholar Hussein Abdillahi, Hussein Abdillahi Search for other works by this author on: Oxford Academic PubMed Google Scholar Jan T. Poolman Jan T. Poolman Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Infectious Diseases, Volume 158, Issue 1, July 1988, Pages 209–212, https://doi.org/10.1093/infdis/158.1.209 Published: 01 July 1988 Article history Received: 26 October 1987 Revision received: 10 February 1988 Published: 01 July 1988
Elevated Blood Cyanide Concentrations in Victims of Smoke Inhalation (1991)

Residential fires may cause cyanide poisoning. At the time of a patient's hospital admission, an elevated plasma lactate concentration is a useful indicator of cyanide toxicity in fire victims who do not have severe burns.
Structure of PqsD, a Pseudomonas Quinolone Signal Biosynthetic Enzyme, in Complex with Anthranilate (2009)

Pseudomonas quinolone signal (PQS), 2-heptyl-3-hydroxy-4-quinolone, is an intercellular alkyl quinolone signaling molecule produced by the opportunistic pathogen Pseudomonas aeruginosa. Alkyl quinolone signaling is an atypical system that, in P. aeruginosa, controls the expression of numerous virulence factors. PQS is synthesized from the tryptophan pathway intermediate, anthranilate, which is derived either from the kynurenine pathway or from an alkyl quinolone specific anthranilate synthase encoded by phnAB. Anthranilate is converted to PQS by the enzymes encoded by the pqsABCDE operon and pqsH. PqsA forms an activated anthraniloyl-CoA thioester that shuttles anthranilate to the PqsD active site where it is transferred to Cys112 of PqsD. In the only biochemically characterized reaction, a condensation then occurs between anthraniloyl-PqsD and malonyl-CoA or malonyl-ACP, a second PqsD substrate, forming 2,4-dihydroxyquinoline (DHQ). The role PqsD plays in the biosynthesis of other alkyl quinolones, such as PQS, is unclear, though it has been reported to be required for their production. No evidence exists that DHQ is a PQS precursor, however. Here we present a structural and biophysical characterization of PqsD that includes several crystal structures of the enzyme, including that of the PqsD-anthranilate covalent intermediate and the inactive Cys112Ala active site mutant in complex with anthranilate. The structure reveals that PqsD is structurally similar to the FabH and chalcone synthase families of fatty acid and polyketide synthases. The crystallographic asymmetric unit contains a PqsD dimer. The PqsD monomer is composed of two nearly identical approximately 170-residue alphabetaalphabetaalpha domains. The structures show anthranilate-liganded Cys112 is positioned deep in the protein interior at the bottom of an approximately 15 A long channel while a second anthraniloyl-CoA molecule is waiting in the cleft leading to the protein surface. Cys112, His257, and Asn287 form the FabH-like catalytic triad of PqsD. The C112A mutant is inactive, although it still reversibly binds anthraniloyl-CoA. The covalent complex between anthranilate and Cys112 clearly illuminates the orientation of key elements of the PqsD catalytic machinery and represents a snapshot of a key point in the catalytic cycle.
Side‐effects of a systemic injection of linear polyethylenimine–DNA complexes (2001)

This study demonstrates that L-PEI/DNA activates the lung endothelium and forms small aggregates, a side-effect linked to the transfection efficiency.
Effect of the dose of oral hydrocortisone on growth rate during long-term treatment of children with salt losing congenital adrenal hyperplasia. (2002)

The effect of the dose of oral hydrocortisone on stature growth rate of patients with the salt losing form of congenital adrenal hyperplasia and adequate electrolyte balance was here assessed. Thirty patients (21 girls and 9 boys) were followed longitudinally for 0.52 to 8.64 years, between chronological ages 0.35 and 8.64 years. Nine consecutive periods (Ps) of follow up were defined in order to compare two auxological parameters, height (H) at the end of a follow up P and delta H standard deviation score (SDS). According to growth rate during a particular P, two types of Ps were defined: Ps with delta H SDS > -0.5 (Group 1, satisfactory growth rate) and Ps with delta H SDS = or < -0.5 (Group 2, poor growth rate). A cut off value of 18.5 mg/m2/day of oral hydrocortisone (95% CI upper limit of group 1) was defined to separate acceptable from excessive doses. In P2, mean (+/- SD) H SDS (-1.81 +/- 1.15) was significantly lower than in any of the other Ps (p < 0.001). In P1 and in P2, delta H SDS was negative, but it was positive in P3 and in P4. Hydrocortisone dose in P1 and in P2 was significantly higher than in the rest of the Ps. All patients in P1 and most patients in P2, but not in other Ps, received excessive doses. Predicted adult H, calculated in 9 patients was not statistically different from their respective target H. It is concluded that, during the first year of life, our patients received an excess of oral hydrocortisone (> 18 mg/m2/day) and grew poorly, but they were able to recover, at least temporarily, when the dose was adjusted during the following years.
The Internal Ribosome Entry Site-Mediated Translation of Antiapoptotic Protein XIAP Is Modulated by the Heterogeneous Nuclear Ribonucleoproteins C1 and C2 (2002)

The X-chromosome-linked inhibitor of apoptosis, XIAP, is the most powerful and ubiquitous intrinsic inhibitor of apoptosis. We have shown previously that the translation of XIAP is controlled by a potent internal ribosome entry site (IRES) element. IRES-mediated translation of XIAP is increased in response to cellular stress, suggesting the critical role for IRES translation during cellular stress. Here, we demonstrate that heterogeneous nuclear ribonucleoproteins C1 and C2 (hnRNPC1 and -C2) are part of the RNP complex that forms on XIAP IRES. Furthermore, the cellular levels of hnRNPC1 and -C2 parallel the activity of XIAP IRES and the overexpression of hnRNPC1 and -C2 specifically enhanced translation of XIAP IRES, suggesting that hnRNPC1 and -C2 may modulate XIAP expression. Given the central role of XIAP in the regulation of apoptosis these results are important for our understanding of the control of apoptosis.
Reliability of a Single-Session Isokinetic and Isometric Strength Measurement Protocol in Older Men (2005)

The test-retest reliability of a single-session isokinetic and isometric strength testing protocol in this group of older healthy men displayed good relative reliability (intraclass correlation coefficient > 0.84); however, because the typical error as a coefficient of variation and ratio limits of agreement (absolute reliability) were large, single-session testing is not recommended.
Salmonella enterica Serovar Typhi Ty21a Expressing Human Papillomavirus Type 16 L1 as a Potential Live Vaccine against Cervical Cancer and Typhoid Fever (2007)

Human papillomavirus (HPV) vaccines based on L1 virus-like particles (VLPs) can prevent HPV-induced genital neoplasias, the precursors of cervical cancer. However, most cervical cancers occur in developing countries, where the implementation of expensive vaccines requiring multiple injections will be difficult. A live Salmonella-based vaccine could be a lower-cost alternative. We previously demonstrated that high HPV type 16 (HPV16)-neutralizing titers are induced after a single oral immunization of mice with attenuated Salmonella enterica serovar Typhimurium strains expressing a codon-optimized version of HPV16 L1 (L1S). To allow the testing of this type of vaccine in women, we constructed a new L1-expressing plasmid, kanL1S, and tested kanL1S recombinants of three Salmonella enterica serovar Typhi vaccine strains shown to be safe in humans, i.e., Ty21a, the actual licensed typhoid vaccine, and two highly immunogenic typhoid vaccine candidates, Ty800 and CVD908-htrA. In an intranasal mouse model of Salmonella serovar Typhi infection, Ty21a kanL1S was unique in inducing HPV16-neutralizing antibodies in serum and genital secretions, while anti-Salmonella responses were similar to those against the parental Ty21a vaccine. Electron microscopy examination of Ty21a kanL1S lysates showed that L1 assembled in capsomers and capsomer aggregates but not well-ordered VLPs. Comparison to the neutralizing antibody response induced by purified HPV16 L1 VLP immunizations in mice suggests that Ty21a kanL1S may be an effective prophylactic HPV vaccine. Ty21a has been widely used against typhoid fever in humans with a remarkable safety record. These finds encourage clinical testing of Ty21a kanL1S as a combined typhoid fever/cervical cancer vaccine with the potential for worldwide application.