gebhart/patrepeval huggingface dataset:
Cancer Risk Estimates for BRCA1 Mutation Carriers Identified in a Risk Evaluation Program (2002)

The estimates for breast and ovarian cancer risk in BRCA1 mutation carriers is higher than population-based estimates but lower than estimates based on families ascertained for linkage studies. These cancer risk estimates may most closely approximate those faced by BRCA1 mutation carriers identified in risk evaluation clinics.
Narrowband lumped-element microstrip filters using capacitively-loaded inductors (1995)

Coupling between microstrip resonators decreases very slowly as a function of the resonator separation. Therefore, it is difficult to realize narrowband filters (e.g., <0.1% fractional bandwidth) in reasonably sized microstrip form due to the very weak coupling values required. In this paper, we report a class of lumped-element filters that uses capacitively-loaded inductors to give frequency-dependent inductance values. A novel frequency transformation technique is used in the design process. Using this approach, filter bandwidth is determined by the inductance slope of frequency-dependent inductors, dL/d/spl omega/. Large coupling capacitance, thus small coupling element separations, can still be used in narrowband microstrip filters to keep the filter layout compact. We present a 5-pole, 0.27% bandwidth YBa/sub 2/Cu/sub 3/O/sub 7/ high-temperature superconducting thin film microstrip prototype filter at 900 MHz, which has 1.2 dB insertion loss and 20 dB return loss. It was designed with the coupling capacitors of a 1% bandwidth filter, and then transformed to a 0.27% fractional bandwidth using an appropriate inductance slope parameter, dL/d/spl omega/. Measurement showed good agreement with theory.
Phase I Trial of Anti-CS1 Monoclonal Antibody Elotuzumab in Combination With Bortezomib in the Treatment of Relapsed/Refractory Multiple Myeloma (2012)

The combination of elotuzumab and bortezomib was generally well-tolerated and showed encouraging activity in patients with relapsed/refractory MM.
Säureamid‐Reaktionen, XLI. Synthese des Oxazols (1964)

Abstract Aus Hydroxyketobernsteinsäure‐ oder Dihydroxyfumarsäureester erhält man mit Formamid in Gegenwart von HCI Oxazol‐dicarbonsäure‐(4.5)‐diester. Das daraus dargestellte Barium‐ oder Natrium‐Salz ergibt mit Chinolinsulfat (in Chinolin) Oxazol.
Mobilization of long-term hematopoietic reconstituting cells in mice by the combination of stem cell factor plus granulocyte colony-stimulating factor (1994)

In this study, we have compared the ability of recombinant human granulocyte colony-stimulating factor (rhG-CSF) alone and the combination of low doses of recombinant rat pegylated stem cell factor (rrSCF-PEG) plus rhG-CSF to mobilize peripheral blood progenitor cells (PBPCs) with long-term engrafting potential. Female recipient irradiated mice were transplanted with PBPCs from male mice that were mobilized with rhG-CSF alone (group A) or rrSCF-PEG plus rhG-CSF (group B). As previously shown, greater short-term survival resulted in group B compared with group A, with 80% and 40% survival at 30 days posttransplant, respectively. Both groups of animals showed long-term donor-derived engraftment in greater than 95% of animals, as determined by quantitative specific polymerase chain reaction amplification of a Y chromosome sequence from whole blood of the mice at 6 to 12 months posttransplantation. Analysis of individual granulocyte-macrophage colonies, picked up from semisolid methylcellulose culture of bone marrow cells from transplanted mice, resulted in detection of donor-derived DNA in 98% of colonies from group B mice compared with 81% from group A mice. These data show that cells with long-term potential are mobilized by rhG-CSF alone and the combination of rrSCF-PEG plus rhG-CSF. Furthermore, an increased number of cells with short-term and long-term engraftment potential was obtained with rrSCF-PEG plus rhG-CSF compared with rhG-CSF alone.
WWW Response Rates to Sociodemographic Items (1999)

In the era of World Wide Web survey usage, it is important for researchers to be aware of characteristics of their respondents. This study examined response rates to demographic items for a web-based survey. Many personal items were asked for on the survey, such as complete name, country of birth and residence, marital status, profession and number of children. Results of the study indicate that once individuals have decided to complete the survey, they are likely to provide most of the information requested.
Production and Characterization of Specific Asialoglycoprotein Receptor Antibodies (1993)

Monoclonal antibodies (MoAbs) were produced by immunizing BALB/c mice with asialoglycoprotein receptor (AGPR) purified from human liver. The purity of AGPR was confirmed by SDS-polyacrylamide gel electrophoresis and by amino acid sequence. An enzyme-linked immunoassay revealed 24 monoclonal antibodies which reacted with human AGPR. By Western blot analysis, all antibodies recognized the 46 kDa human AGPR under the non-reduced conditions and four MoAbs recognized reduced protein. Two MoAbs reacted with AGPR derived from rat, rabbit and mouse liver under both non-reduced and reduced conditions, suggesting that we could obtain antibodies which reacted with AGPR epitopes shared by different species. In immunohistochemical studies, 30201-MoAb reacted with human liver tissue but not with other tissues. This antibody immunoprecipitated two major bands of 46 kDa and 39 kDa from [35S]-methionine metabolically labeled human hepatoma HepG2 cells. The determinant recognized by 30201-MoAb is a common epitope of AGPR which is present in different species and in both the precursor and maturation forms of the receptor.
In Situ Zymography (2010)

In situ zymography is a unique laboratory technique that enables the localisation of matrix-degrading metalloproteinase (MMP) activity in histological sections. Frozen sections are placed on glass slides coated with fluorescently labelled matrix proteins. After incubation MMP activity can be observed as black holes in the fluorescent background due to proteolysis of the matrix protein. Alternatively frozen sections can be incubated with matrix proteins conjugated to quenched fluorescein. Proteolysis of the substrate by MMPs leads to the release of fluorescence. This technique can be combined with immunohistochemistry to enable co-location of proteins such as cell type markers or other proteins of interest. Additionally, this technique can be adapted for use with cell cultures, permitting precise location of MMP activity within cells, time-lapse analysis of MMP activity and analysis of MMP activity in migrating cells.
HLA antibodies as a cause of false‐positive reactions in screening enzyme immunoassays for antibodies to human T‐lymphotropic virus Type III (1986)

15,680 volunteer blood donors were screened by enzyme-linked immunosorbent assay (EIA) for antibodies to human T-lymphotropic virus Type III (HTLV-III). On at least two of three determinations, 0.37 percent were found to be reactive. When the first 38 of these samples were sent for repeat EIA and Western blot, the EIA was positive in 14. In only three of these 14 samples was the EIA result confirmed by Western blot. Eight of these eleven false-positive samples were from women who were found to have HLA antibodies. In seven of these women, the HLA antibodies were directed to Class II antigens expressed on the cell used to grow HTLV-III in the preparation of screening EIA kits. We conclude that HLA antibodies are an important cause of false-positive reactions in the screening test for HTLV-III antibody.
EGFR wild-type amplification and activation promote invasion and development of glioblastoma independent of angiogenesis (2013)

Angiogenesis is regarded as a hallmark of cancer progression and it has been postulated that solid tumor growth depends on angiogenesis. At present, however, it is clear that tumor cell invasion can occur without angiogenesis, a phenomenon that is particularly evident by the infiltrative growth of malignant brain tumors, such as glioblastomas (GBMs). In these tumors, amplification or overexpression of wild-type (wt) or truncated and constitutively activated epidermal growth factor receptor (EGFR) are regarded as important events in GBM development, where the complex downstream signaling events have been implicated in tumor cell invasion, angiogenesis and proliferation. Here, we show that amplification and in particular activation of wild-type EGFR represents an underlying mechanism for non-angiogenic, invasive tumor growth. Using a clinically relevant human GBM xenograft model, we show that tumor cells with EGFR gene amplification and activation diffusely infiltrate normal brain tissue independent of angiogenesis and that transient inhibition of EGFR activity by cetuximab inhibits the invasive tumor growth. Moreover, stable, long-term expression of a dominant-negative EGFR leads to a mesenchymal to epithelial-like transition and induction of angiogenic tumor growth. Analysis of human GBM biopsies confirmed that EGFR activation correlated with invasive/non-angiogenic tumor growth. In conclusion, our results indicate that activation of wild-type EGFR promotes invasion and glioblastoma development independent of angiogenesis, whereas loss of its activity results in angiogenic tumor growth.
Identification of Tumor Necrosis Factor (TNF) Amino Acids Crucial for Binding to the Murine p75 TNF Receptor and Construction of Receptor-selective Mutants (2001)

The bioactivity of tumor necrosis factor (TNF) is mediated by two TNF receptors (TNF-Rs), more particularly TNF-RI and TNF-RII. Although human TNF (hTNF) and murine TNF (mTNF) are very homologous, hTNF binds only to mTNF-RI. By measuring the binding of a panel of mTNF/hTNF chimeras to both mTNF-R, we pinpointed the TNF region that mediates the interaction with mTNF-RII. Using site-specific mutagenesis, we identified amino acids 71-73 and 89 as the main interacting residues. Mutein hTNF-S71D/T72Y/H73 Delta/T89E interacts with both types of mTNF-R and is active in CT6 cell proliferation assays mediated by mTNF-RII. Mutein mTNF-D71S/Y72T/Delta 73H/E89T binds to mTNF-RI only and is no longer active on CT6 cells. However, the L929s cytotoxicity of this mutein (an effect mediated by mTNF-RI triggering) was also 100-fold lower than that of wild-type mTNF due to enhanced dissociation during incubation at subnanomolar concentrations. The additional mutation of amino acid 102, resulting in the mutein mTNF-D71S/Y72T/Delta 73H/E89T/P102Q, restored the trimer stability, which led to an enhanced specific activity on L929s cells. Hence the specific activity of a TNF species is governed not only by its receptor binding characteristics but also by its trimer stability after incubation at subnanomolar concentrations. In conclusion, the mutation of TNF amino acids 71-73, 89, and 102 is sufficient to obtain a mTNF mutein selective for mTNF-RI and a hTNF mutein that, unlike wild-type hTNF, also acts on mTNF-RII.
Dichotomy in the human CD4+ T‐cell response to Leishmania parasites (1994)

Leishmania parasites cause human diseases ranging from self-healing cutaneous ulcers to fatal systemic infections. In addition, many individuals become infected without developing disease. In mice the two subsets of CD4+ T cells, Th1 and Th2, have different effects on the outcome of experimental Leishmania infections. Th1 cells producing interferon-gamma (IFN-gamma) mediate resistance, whereas Th2 cells producing interleukin-4 (IL-4) and IL-10 are associated with susceptibility and exacerbation. Evidence is accumulating that a Th1/Th2 dichotomy in the T-cell response to Leishmania exists also in humans, and that the balance between subsets of parasite-specific T cells may play an important regulatory role in determining the outcome of the infections.
Synthesis of new bifunctional compounds which selectively alkylate guanines in DNA. (1998)

The aim of this work was to develop new bifunctional alkylating agents which damage DNA in a selective manner. In order to extend our previously published work on conformationally restricted nitrogen mustards containing one piperidine ring, new bispiperidine derivatives were designed with varying lengths of carbon chain between the two rings and structure-activity relationships in these systems were studied. Thus samples of new bispiperidine salts 22-26 with chloromethyl groups at the 2-positions and a bridge between the two nitrogen atoms of 2-6 carbon atoms were synthesized. The analogous new bis(p-nitrophenylcarbamates) 17-21 were also prepared. The free bases were designed to be bifunctional alkylating agents via aziridinium ion formation with different distances between the two alkylating sites. The bispiperidines 22-24 were shown to alkylate guanines at the 7-position in the major groove of DNA more selectively than melphalan. The bispiperidine 22 with the shortest two carbon bridge was the most reactive but it was less cytotoxic than melphalan in a human colon carcinoma cell line (IC50 value approximately 30 microM) and in a human chronic myeloid leukaemia cell line (IC50 value approximately 12 microM). The most cytotoxic compound in the latter cell line was the carbamate 17, with an IC50 value of approximately 0.3 microM, and carbamates 17, 19 and 20 were most potent in a panel of human ovarian carcinoma cell lines. These compounds also showed circumvention of acquired cisplatin resistance in three paired cell lines. The carbamates 17-21, however, were less efficient at alkylating and cross-linking naked DNA than the bispiperidines 22-26.
Learning Video Object Segmentation with Visual Memory (2017)

This paper addresses the task of segmenting moving objects in unconstrained videos. We introduce a novel two-stream neural network with an explicit memory module to achieve this. The two streams of the network encode spatial and temporal features in a video sequence respectively, while the memory module captures the evolution of objects over time. The module to build a “visual memory” in video, i.e., a joint representation of all the video frames, is realized with a convolutional recurrent unit learned from a small number of training video sequences. Given a video frame as input, our approach assigns each pixel an object or background label based on the learned spatio-temporal features as well as the "visual memory" specific to the video, acquired automatically without any manually-annotated frames. The visual memory is implemented with convolutional gated recurrent units, which allows to propagate spatial information over time. We evaluate our method extensively on two benchmarks, DAVIS and Freiburg-Berkeley motion segmentation datasets, and show state-of-the-art results. For example, our approach outperforms the top method on the DAVIS dataset by nearly 6%. We also provide an extensive ablative analysis to investigate the influence of each component in the proposed framework.
FcγRIIIa-158V/F Polymorphism Influences the Binding of IgG by Natural Killer Cell FcγRIIIa, Independently of the FcγRIIIa-48L/R/H Phenotype (1997)

We analyzed a genetic polymorphism of Fc gamma receptor IIIa (CD16) that is present on position 158 (Phe or Val) in the membrane-proximal, IgG-binding domain. With a polymerase chain reaction-based allele-specific restriction analysis assay we genotyped 87 donors and found gene frequencies of 0.57 and 0.43 for Fc gammaRIIIA-158F and -158V, respectively. A clear linkage was observed between the Fc gammaRIIIA-158F and -48L genotypes on the one hand and the Fc gammaRIIIA-158V and -48H or -48R genotypes on the other hand (chi2 test; P < .001). To determine the functional consequences of this Fc gammaRIIIa-158V/F polymorphism, we performed IgG binding experiments with natural killer (NK) cells from genotyped donors. All donors were also typed for the recently described triallelic Fc gammaRIIIa-48L/R/H polymorphism. NK cells were treated with lactic acid to remove cell-associated IgG. Fc gammaRIIIa(NK)-158F bound significantly less IgG1, IgG3, and IgG4 than did Fc gammaRIIIa(NK)-158V, irrespective of the Fc gammaRIIIa-48 phenotype. Moreover, freshly isolated NK cells from Fc gammaRIIIa-158VV individuals carried significantly more cytophilic IgG than did NK cells from Fc gammaRIIIa-158FF individuals. In addition, CD16 monoclonal antibody (MoAb) MEM154 bound more strongly to Fc gammaRIIIa-158V, compared with -158F, again independently of the Fc gammaRIIIa-48 phenotype. The binding of MoAb B73.1 was not influenced by the Fc gammaRIIIa-158V/F polymorphism, but proved to depend solely on the amino acid present at position 48 of Fc gammaRIIIa. In conclusion, the previously reported differences in IgG binding among the three Fc gammaRIIIa-48L/R/H isoforms are a consequence of the linked, biallelic Fc gammaRIIIa-158V/F polymorphism at amino-acid position 158.
Cache-conscious data placement (1998)

As the gap between memory and processor speeds continues to widen, cache eficiency is an increasingly important component of processor performance. Compiler techniques have been used to improve instruction cache pet$ormance by mapping code with temporal locality to different cache blocks in the virtual address space eliminating cache conflicts. These code placement techniques can be applied directly to the problem of placing data for improved data cache pedormance.In this paper we present a general framework for Cache Conscious Data Placement. This is a compiler directed approach that creates an address placement for the stack (local variables), global variables, heap objects, and constants in order to reduce data cache misses. The placement of data objects is guided by a temporal relationship graph between objects generated via profiling. Our results show that profile driven data placement significantly reduces the data miss rate by 24% on average.
Unsupervised word sense disambiguation rivaling supervised methods (1995)

This paper presents an unsupervised learning algorithm for sense disambiguation that, when trained on unannotated English text, rivals the performance of supervised techniques that require time-consuming hand annotations. The algorithm is based on two powerful constraints -that words tend to have one sense per discourse and one sense per collocation -exploited in an iterative bootstrapping procedure. Tested accuracy exceeds 96%.
Serum β-2 microglobulin predicts mortality in people with diabetes (2013)

Serum B2M level is a novel predictor of all-cause and diabetes-related mortality in people with diabetes regardless of renal function.
Taxol Content in Bark, Wood, Root, Leaf, Twig, and Seedling from Several Taxus Species (1990)

Taxol content in various parts of several Taxus species have been determined. The weight percent ranged from 0.00003 to 0.069.
High EphA2 protein expression in renal cell carcinoma is associated with a poor disease outcome (2014)

The receptor tyrosine kinase, ephrin type-A receptor 2 (EphA2), is normally expressed at sites of cell-to-cell contact in adult epithelial tissues, however, recent studies have shown that it is also overexpressed in various types of epithelial carcinomas, with the greatest level of EphA2 expression observed in metastatic lesions. In the present study, the association between the expression of EphA2 and the outcome of RCC patients was assessed. The high expression level of EphA2 was identified by log-rank test for a statistically significant prediction of the RCC outcome. In an overall multivariate analysis, the high expression level of EphA2 was identified as an independent predictor of RCC outcome. The length of survival of the patients with high EphA2 expression was shorter than that of the patients with a low level of expression (relative risk, 2.304; 95% CI, 1.102-4.818; P=0.027). The analysis of the expression levels of EphA2 in tumor tissues may aid in the identification of the patient subgroup that are at a high risk of a poor disease outcome.
Endoplasmic Reticulum Chaperone Protein GRP78 Protects Cells from Apoptosis Induced by Topoisomerase Inhibitors (2003)

A large number of correlative studies have established that the activation of the unfolded protein response (UPR) alters the cell's sensitivity to chemotherapeutic agents. Although the induction of the glucose-regulated proteins (GRPs) is commonly used as an indicator for the UPR, the direct role of the GRPs in conferring resistance to DNA damaging agents has not been proven. We report here that without the use of endoplasmic reticulum (ER) stress inducers, specific overexpression of GRP78 results in reduced apoptosis and higher colony survival when challenged with topoisomerase II inhibitors, etoposide and doxorubicin, and topoisomerase I inhibitor, camptothecin. While investigating the mechanism for the GRP78 protective effect against etoposide-induced cell death, we discovered that in contrast to the UPR, GRP78 overexpression does not result in G1 arrest or depletion of topoisomerase II. Caspase-7, an executor caspase that is associated with the ER, is activated by etoposide. We show here that specific expression of GRP78 blocks caspase-7 activation by etoposide both in vivo and in vitro, and this effect can be reversed by addition of dATP in a cell-free system. Recently, it was reported that ectopically expressed GRP78 and caspases-7 and -12 form a complex, thus coupling ER stress to the cell death program. However, the mechanism of how GRP78, a presumably ER lumen protein, can regulate cytosolic effectors of apoptosis is not known. Here we provide evidence that a subpopulation of GRP78 can exist as an ER transmembrane protein, as well as co-localize with caspase-7, as confirmed by fluorescence microscopy. Co-immunoprecipitation studies further reveal endogenous GRP78 constitutively associates with procaspase-7 but not with procaspase-3. Lastly, a GRP78 mutant deleted of its ATP binding domain fails to bind procaspase-7 and loses its protective effect against etoposide-induced apoptosis.
SYNTHESIS OF SOME NEW THIADIAZOLE, SELENA, TRIAZINE, THIAZOLE AND CYANOPYRIDINE DERIVATIVES WITH ASSAY FOR THEIR ANTITUMOR ACTIVITY (1996)

Abstract The synthesis of 2-methyl-3-(4′-acetylphenyl)-quinazol-4-one II was accomplished by the condensation of the benzoxazine I with 4-aminoacetophenone. The semicarbazone III was obtained from II by refluxing with an equimolar amount of semicarbazide hydrochloride in ethanol. The latter compound was then subjected to oxidative cyclization either by thionyl chloride or selenium dioxide to give thiadiazole and selena derivatives IV and V, respectively. Compound V was condensed with different aromatic amines to yield the corresponding Schiff's bases VI. The phenylglyoxal derivative VII was prepared by oxidation of II with selenium dioxide. Condensation of VII with thiosemicarbazide in ethanol in the presence of potassium carbonate gave 3-mercapto-1,2,4-triazine derivative VIII. Treatment of II with bromine in acetic acid gave the bromo derivative IX which was reacted with thiourea to give 2-aminothiazole derivative X. Cyanopyridine-2-(1H)-thione derivatives XIVa-h were obtained via the reaction of arylmethylenecyanothioacetamide XI with active methylene carbonyl compound II. Assay for antitumor activity showed that compound XIVc has a significant activity against Ehrlich Ascites Carcinoma tumor cells (in vitro) and displayed a significant percent of the non viable tumor cells to about 40% and 80% at concentration of 10 and 100 ng, respectively.