gebhart/patrepeval huggingface dataset:
Volume holographic memory systems: techniques and architectures (1995)

Although the concept of using multiplexed holography for data storage has been considered for some time, recent advances in several critical device technologies along with developments in storage materials have greatly enhanced the likelihood of successful implementations. We review several basic architectural concepts along with various multiplexing options and associated techniques for holographic data storage.
Gα i1 and Gα i3 Are Required for Epidermal Growth Factor–Mediated Activation of the Akt-mTORC1 Pathway (2009)

The precise mechanism whereby epidermal growth factor (EGF) activates the serine-threonine kinase Akt and the mammalian target of rapamycin (mTOR) complex 1 (mTORC1) remains elusive. Here, we report that the alpha subunits of the heterotrimeric guanine nucleotide-binding proteins (G proteins) Galpha(i1) and Galpha(i3) are critical for this activation process. Both Galpha(i1) and Galpha(i3) formed complexes with growth factor receptor binding 2 (Grb2)-associated binding protein 1 (Gab1) and the EGF receptor (EGFR) and were required for the phosphorylation of Gab1 and its subsequent interaction with the p85 subunit of phosphatidylinositol 3-kinase in response to EGF. Loss of Galpha(i1) and Galpha(i3) severely impaired the activation of Akt and of p70 S6 kinase and 4E-BP1, downstream targets of mTORC1, in response to EGF, heparin-binding EGF-like growth factor, and transforming growth factor alpha, but not insulin, insulin-like growth factor, or platelet-derived growth factor. In addition, ablation of Galpha(i1) and Galpha(i3) largely inhibited EGF-induced cell growth, migration, and survival and the accumulation of cyclin D1. Overall, this study suggests that Galpha(i1) and Galpha(i3) lie downstream of EGFR, but upstream of Gab1-mediated activation of Akt and mTORC1, thus revealing a role for Galpha(i) proteins in mediating EGFR signaling.
Secretory Leukocyte Protease Inhibitor Binds to Annexin II, a Cofactor for Macrophage HIV-1 Infection (2004)

The distribution of secretory leukocyte protease inhibitor (SLPI) at entry portals indicates its involvement in defending the host from pathogens, consistent with the ability of SLPI to inhibit human immunodeficiency virus (HIV)-1 infection by an unknown mechanism. We now demonstrate that SLPI binds to the membrane of human macrophages through the phospholipid-binding protein, annexin II. Based on the recent identification of human cell membrane phosphatidylserine (PS) in the outer coat of HIV-1, we define a novel role for annexin II, a PS-binding moiety, as a cellular cofactor supporting macrophage HIV-1 infection. Moreover, this HIV-1 PS interaction with annexin II can be disrupted by SLPI or other annexin II-specific inhibitors. The PS-annexin II connection may represent a new target to prevent HIV-1 infection.
Beyond Silica: Nonoxidic Mesostructured Materials (2007)

Abstract Recent advances in the design and synthesis of mesostructured and mesoporous materials with nonoxide frameworks are reviewed. Chalcogenides comprise the most important class, and all possible pore arrangements known for silica can be achieved in these materials. The special molecular chemistry that leads to organized nonoxidic mesostructures that have a variety of compositions, pore sizes, and semiconductor bandgaps is presented. Also reviewed are recent developments in mesoporous elements, such as metals and germanium. The goal in research on mesoporous semiconductors is to create materials scaffolds with new physical phenomena that derive from the combined characteristics of long‐range porosity and electronic and optical properties.
Recombinant Factor VIIa Is Effective for Bleeding and Surgery in Patients With Glanzmann Thrombasthenia (1999)

Recombinant activated factor VII (rFVIIa) was found to be effective and safe in treating 24 bleeding episodes and to prevent bleeding during one bilateral herniorrhaphy in four children with Glanzmann thrombasthenia. One of the patients had alloantibodies to platelet membrane glycoprotein (GP) IIb/IIIa and was refractory to platelet transfusion. rFVIIa was administered at 89 to 116 microg/kg per injection every 2 hours, in association with antifibrinolytic drugs. Bleeding stopped in all cases, but platelet transfusion was required in one. Two bleeding episodes recurred 36 and 63 hours after discontinuation of rFVIIa, but were successfully treated with additional doses. No adverse effects of rFVIIa were observed. Although the number of patients is small, our study suggests that rFVIIa may be an alternative to platelet transfusions in patients with a severe congenital thrombocytopathy.
Phase II Randomized Study of Vaccine Treatment of Advanced Prostate Cancer (E7897): A Trial of the Eastern Cooperative Oncology Group (2004)

Therapy with poxviruses expressing PSA and delivered in a prime/boost regimen was feasible and associated with minimal toxicity in the cooperative group setting. A significant proportion of men remained free of PSA and clinical progression after 19 months follow-up, and nearly half demonstrated an increase in PSA-specific T-cell responses. Phase III studies are needed to define the role of vaccination in men with prostate cancer or those who are at risk for the disease.
Glucose/O<sub>2</sub> Biofuel Cell Operating at Physiological Conditions (2002)

A glassy carbon electrode modified with bilirubin oxidase (BOD) cross-linked to an Os redox polymer functions as a bio-cathode for a 4-electron reduction of O2 under neutral conditions. On the other hand, a glassy carbon electrode modified with pyrroloquinoline quinone-dependent soluble glucose dehydrogenase (sGDH) or glucose oxidase cross-linked to an Os redox polymer functions as a bio-anode for a 2-electron oxidation of glucose. A prototype of a one-compartment glucose/O2 biofuel cell without a separator was constructed by using the BOD-modified cathode and the sGDH-modified anode. The maximum power density was 0.058 mW cm−2. The loss in the power is discussed in terms of thermodynamics and kinetics.
Cellular events associated with the induction of bone by demineralized bone (1989)

Implantation of demineralized bone (DB) in the form of powder or intact segments in extra skeletal sites stimulates new bone formation. Urist and co-workers presented substantial evidence that there is a noncollagenous protein that has the ability to induce bone formation. One aim of this study was to trace the process of bone formation when DB, in the form of perforated rectangular plates, is implanted subcutaneously in 2-month-old rats. A second objective was to determine whether cartilage cells play a role in the formation of bone in this model. Various DB plates with 0.25 mm diameter holes were implanted subcutaneously for 1-4 weeks in rats. One week after implantation, DB plates were covered by vascularized connective tissue that invaded the perforations. Aggregates of chondrocytes were observed within the holes and on periosteal surfaces in only a few specimens. Further cartilage proliferation was not observed, and by the 2nd week there was no evidence of endochondral bone formation. Where these cartilage-like cells were present, a thin layer of mineral was deposited around them; resorption and fibrous tissue infiltration followed. This aborted form of endochondral calcification was not followed spatially by bone formation. Patent vascularized channels were invaded by alkaline phosphatase-positive mononuclear cells and fibroblasts, and became enlarged by the enzymatic action of macrophages. The next step involved the calcification of DB plates adjacent to the wide spaces. Osteoclasts now appeared leading to the resorption of this recalcified matrix. The eroded and now enlarged lacunar surfaces were lined by newly formed bone and osteoblasts. This process continued so that, at the end of 4 weeks following implantation, the original DB plates were replaced by trabecular bone. Biochemical data on calcium and alkaline phosphatase levels in the implants paralleled the morphological observations.
Asymmetric Michael Additions to Nitroalkenes (2002)

The asymmetric conjugate addition of various carbon and heteroatom nucleophiles to nitroalkenes as a tool for the construction of highly functionalized synthetic building blocks is presented. Diastereoselective, substrate-controlled 1,4-additions are also included. Besides auxiliary controlled asymmetric Michael additions, external asymmetric versions employing enantiopure additives, addition-elimination processes with enantiopure leaving groups, and catalytic asymmetric syntheses are described. The use of the highly reactive nitroalkenes as Michael acceptors opens the way to synthetically very useful C−C and C−X bond-forming reactions and subsequent transformations as is demonstrated by various applications. (© Wiley-VCH Verlag GmbH, 69451 Weinheim, Germany, 2002)
Comparison of two non‐parametric models for daily traffic forecasting in Hong Kong (2006)

Abstract The most up‐to‐date annual average daily traffic (AADT) is always required for transport model development and calibration. However, the current‐year AADT data are not always available. The short‐term traffic flow forecasting models can be used to predict the traffic flows for the current year. In this paper, two non‐parametric models, non‐parametric regression (NPR) and Gaussian maximum likelihood (GML), are chosen for short‐term traffic forecasting based on historical data collected for the annual traffic census (ATC) in Hong Kong. These models are adapted as they are more flexible and efficient in forecasting the daily vehicular flows in the Hong Kong ATC core stations (in total of 87 stations). The daily vehicular flows predicted by these models are then used to calculate the AADT of the current year, 1999. The overall prediction and comparison results show that the NPR model produces better forecasts than the GML model using the ATC data in Hong Kong. Copyright © 2006 John Wiley _ Sons, Ltd.
Prophylaxis and acute therapy of arterial embolism with special reference to cerebral embolism. (1991)

Prophylaxis and treatment of arterial embolism in high-risk patients includes therapy with antiplatelet drugs, anticoagulation, and vascular surgery. The prominent causes of cerebral ischemia are intraarterial emboli from atheromatous plaques and cardiac emboli. In patients with recent hemispheric transient ischemic attacks or minor stroke and ipsilateral high-grade internal carotid artery stenosis of 70 to 99% carotid endarterectomy has shown to be effective in prevention of major stroke or death. In the majority of patients with moderate atherosclerotic disease of the extracranial arteries as well as in patients with a cardiac source of emboli, no generally excepted therapy for primary and secondary prevention of cerebral ischemia or systemic embolism exists. The efficacy of antiplatelet drugs and anticoagulants in these patients is still investigated in a number of clinical multicenter studies. From the presently available data one can conclude that the antiplatelet agent acetylsalicylic acid in a dosage of 300 mg per day is effective in the secondary prevention of stroke and death in patients with preceding transient ischemic attacks, minor or major stroke and suspected artery-to-artery embolism from mild to moderate atherothrombotic carotid and vertebral artery disease. If there are no contraindications, we recommend anticoagulation in recurrent transient ischemic attacks not responding to antiplatelet drugs, in progressing stroke especially in the vertebrobasilar territory, in transient ischemic attacks in patients with rheumatic atrial fibrillation and left atrium thrombi, in minor stroke and proven cardiac embolism, in cerebral ischemia due to traumatic large vessel disease, and before and following elective cardioversion in patients with long-standing atrial fibrillation. A therapeutic dilemma still exists in patients with nonrheumatic atrial fibrillation; the presently available data are not sufficient to give recommendations whether aspirin or anticoagulants should be given for primary and secondary prevention of stroke and systemic embolism in these patients.
Controlling alternate routing in general-mesh packet flow networks (1994)

High-speed packet networks will begin to support services that need Quality-of-Service (QoS) guarantees. Guaranteeing QoS typically translates to reserving resources for the duration of a call. We propose a state-dependent routing scheme that builds on any base state-independent routing scheme, by routing flows which are blocked on their primary paths (as selected by the state-independent scheme) onto alternate paths in a manner that is guaranteed—under certain Poisson assumptions— to improve on the performance of the base state-independent scheme. Our scheme only requires each node to have state information of those links that are incident on it. Such a scheme is of value when either the base state-independent scheme is already in place and a complete overhaul of the routing algorithm is undesirable, or when the state (reserved flows) of a link changes fast enough that the timely update of state information is infeasible to all possible call-originators. The performance improvements due to our controlled alternate routing scheme are borne out from simulations conducted on a fully-connected 4-node network, as well as on a sparsely-connected 12-node network modeled on the NSFNet T3 Backbone.
InGaN/GaN/AlGaN-based laser diodes with modulation-doped strained-layer superlattices grown on an epitaxially laterally overgrown GaN substrate (1998)

InGaN multi-quantum-well-structure laser diodes with Al0.14Ga0.86N/GaN modulation doped strained-layer superlattice cladding layers grown on an epitaxially laterally overgrown GaN (ELOG) substrate was demonstrated to have a lifetime of more than 1150 h under room-temperature continuous-wave operation. After 4 μm etching of the ELOG substrate, the etch pit density was about 2×108 cm2 in the region of the 4-μm-wide stripe window, but almost zero in the region of the 7-μm-wide SiO2 stripe.
Effect of Lapatinib on the Development of Estrogen Receptor–Negative Mammary Tumors in Mice (2009)

Lapatinib, a selective orally available inhibitor of epidermal growth factor receptor (EGFR) and ErbB2 receptor tyrosine kinases, is a promising agent for the treatment of breast cancer. We examined the effect of lapatinib on the development of mammary tumors in MMTV-erbB2 transgenic mice, which express wild-type ErbB2 under the control of the mouse mammary tumor virus promoter and spontaneously develop estrogen receptor (ER)-negative and ErbB2-positive mammary tumors by 14 months of age. Mice were treated from age 3 months to age 15 months with vehicle (n = 17) or lapatinib (30 or 75 mg/kg body weight; n = 16 mice per group) by oral gavage twice daily (6 d/wk). All statistical tests were two-sided. By 328 days after the start of treatment, all 17 (100%) of the vehicle-treated mice vs five (31%) of the 16 mice treated with high-dose lapatinib developed mammary tumors (P < .001). Among MMTV-erbB2 mice treated for 5 months (n = 20 mice per group), those treated with lapatinib had fewer premalignant lesions and noninvasive cancers in their mammary glands than those treated with vehicle (P = .02). Lapatinib also effectively blocked epidermal growth factor-induced signaling through the EGFR and ErbB2 receptors, suppressed cyclin D1 and epiregulin mRNA expression, and stimulated p27 mRNA expression in human mammary epithelial cells and in mammary epithelial cells from mice treated for 5 months with high-dose lapatinib. Thus, cyclin D1, epiregulin, and p27 may represent useful biomarkers of lapatinib response in patients. These data suggest that lapatinib is a promising agent for the prevention of ER-negative breast cancer.
Identification and amino acid sequence of the deoxynucleoside triphosphate binding site in Escherichia coli DNA polymerase I (1987)

We have labeled the large fragment of Escherichia coli DNA polymerase I (Pol I) with pyridoxal 5'-phosphate, a substrate binding site directed reagent for DNA polymerases [Modak, M. J. (1976) Biochemistry 15, 3620-3626]. A covalent attachment of pyridoxal phosphate to Pol I results in the loss of substrate binding as well as the polymerase activity. The inactivation was found to be strictly dependent on the presence of a divalent metal ion. Four moles of pyridoxal phosphate was found to react per mole of the enzyme, while in the presence of substrate deoxynucleoside triphosphate only 3 mol of pyridoxal phosphate was bound. To identify the substrate-protected site on the enzyme, tryptic peptides from enzyme labeled with pyridoxal phosphate and tritiated borohydride, in the presence and absence of substrate, were resolved on a C-18 reverse-phase column. A single peptide containing the substrate-protected site was identified and further purified. The amino acid composition and sequence analysis of this peptide revealed it to span residues 756-775 in the primary acid sequence of Pol I. Lys-758 of this sequence was found to be the site of the pyridoxal phosphate reaction. It is therefore concluded that Lys-758 is the site of binding for the metal chelate form of nucleotide substrates in E. coli DNA polymerase I.
The Synthesis, Structural Characterization, and Receptor Specificity of the α-Conotoxin Vc1.1 (2006)

The alpha-conotoxin Vc1.1 is a small disulfide-bonded peptide currently in development as a treatment for neuropathic pain. This study describes the synthesis, determination of the disulfide connectivity, and the determination of the three-dimensional structure of Vc1.1 using NMR spectroscopy. Vc1.1 was shown to inhibit nicotine-evoked membrane currents in isolated bovine chromaffin cells in a concentration-dependent manner and preferentially targets peripheral nicotinic acetylcholine receptor (nAChR) subtypes over central subtypes. Specifically, Vc1.1 is selective for alpha3-containing nAChR subtypes. The three-dimensional structure of Vc1.1 comprises a small alpha-helix spanning residues Pro6 to Asp11 and is braced by the I-III, II-IV disulfide connectivity seen in other alpha-conotoxins. A comparison of the structure of Vc1.1 with other alpha-conotoxins, taken together with nAChR selectivity data, suggests that the conserved proline at position 6 is important for binding, whereas a number of residues in the C-terminal portion of the peptide contribute toward the selectivity. The structure reported here should open new opportunities for further development of Vc1.1 or analogues as analgesic agents.
Digital television: a perspective (1995)

Commercial television was standardized originally mainly for entertainment, sports, and news, using over-the-air broadcasts. While a few upward compatible changes have been added over the years, the standard has remained essentially unchanged. Moreover, no other standard has had so many products and services built around it and withstood such a period of rapid technological change. Digital television promises to disturb the current equilibrium in consumer television and in the broader media industries. It is forcing a convergence with the simultaneous expansion of personal computing into the home and the commercialization of research networks. Digitizing television in a cost-effective manner and integrating it with computers, telecommunication networks, and consumer products will produce a large array of new products and services. The paper describes digital television, factors affecting it, its potential, and some major issues in its evolution.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Improved Quadrature Duobinary System Performance Using Multi-Modulus Equalization (2013)

Quadrature duobinary (QDB) signals can be generated from strongly filtered quadrature phase shift keying whose constellation becomes a 9-ary quadrature amplitude modulation. In this letter, the performance of multi-modulus equalization (MMEQ) to suppress noise and crosstalk for this QDB signal is experimentally investigated and compared with that of the constant-modulus equalization (CMEQ) with post filtering, under different filter bandwidth and carrier spacing. The results show that the MMEQ scheme has better performance for noise and crosstalk suppression, resulting in improved filtering tolerance. This improved filtering tolerance is demonstrated in an 8-channel 112-Gb/s wavelength-division-multiplexing experiment with a 25-GHz grid over 2640-km single-mode fiber. Compared with the regular CMEQ with the post filtering scheme, 1-dB optical signal-to-noise ratio improvement at the bit-error-ratio (BER) of <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$1\times 10^{-3}$</tex></formula> and 32% increased transmission distance at the BER level of <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$3.8\times 10^{-3}$</tex> </formula> for forward-error-correction are obtained for the MMEQ scheme.
Three‐dimensional structure of fatty‐acid‐binding protein from bovine heart (1991)

The complex of fatty-acid-binding protein (FABP) from bovine heart (cFABP, pI4.9) with endogenous lipid was crystallized in the presence of ammonium sulfate as precipitant. The needle-shaped crystals belong to the monoclinic space group C2, with unit-cell constants a = 5.262(6) nm, b = 7.631(8) nm, c = 3.945(5) nm and beta = 94.47(9) degrees. A native data set to 0.35 nm resolution was collected using synchrotron radiation and film methods. An initial model for the three-dimensional structure of the protein was constructed based on the crystal structure of the related bovine P2 myelin protein [Jones, T.A., Bergfors, T., Sedzik, J. & Unge, T. (1988) EMBO J. 7, 1597-1604] to which the amino acid sequence of bovine cFABP was adapted. Energy minimizations were carried out under different conditions using both an all-atom and a united-atom force field. The optimized models were used to determine the crystal structure of cFABP by molecular-replacement techniques. The structure was refined by simulated annealing to R = 0.267. As the bound lipid is heterogeneous, it could not be located in the electron-density map and/or the attained resolution was not sufficient. Bovine cFABP is composed of ten antiparallel beta strands forming a beta barrel, and by two alpha helices. The structural features are similar to those of other members of the superfamily of hydrophobic molecule transporters.
Atmospheric Concentrations of HCl, HONO, HNO3, SO2 and NH3 in Auckland, New Zealand (2004)

The atmospheric concentrations of HCl, HONO, HNO3, SO2 and NH3 have been measured at an urban background site in Auckland, New Zealand in the summer and winter of 2003. Their median concentrations (pptv) were 31, 90, 34, 119 and 1,560 respectively in the summer and 12, 306, 37, 127 and 1,852 in the winter. Previous measurements of these gases in New Zealand are lacking. HONO experienced the most pronounced seasonal difference, having significantly higher wintertime concentrations, NH3 had only slightly higher concentrations in winter, however in contrast HCl demonstrated an opposite seasonal trend (significantly higher summertime concentrations), while HNO3 and SO2 did not have any apparent seasonal variations. Although in the summer these gaseous species did not show significant differences in their atmospheric concentrations between weekdays and weekends, in the winter the atmospheric concentrations of HONO, HNO3, SO2 and NH3 were significantly higher on weekdays than on weekends. It is proposed that in the Auckland summer, vehicle emissions are the dominant source of HONO, HNO3, and NH3, but that most SO2 is produced from both vehicular and marine sources. However in winter it appears that all but HCl had major vehicle-related sources. It is also suggested that degassing of chloride from sea salts could be the major source of HCl at this site.