gebhart/patrepeval huggingface dataset:
A Chromodomain Protein Encoded by the Arabidopsis CAO Gene Is a Plant-Specific Component of the Chloroplast Signal Recognition Particle Pathway That Is Involved in LHCP Targeting (1999)

A recessive mutation in Arabidopsis, named chaos (for chlorophyll a/b binding protein harvesting-organelle specific; designated gene symbol CAO), was isolated by using transposon tagging. Characterization of the phenotype of the chaos mutant revealed a specific reduction of pigment binding antenna proteins in the thylakoid membrane. These nuclear-encoded proteins utilize a chloroplast signal recognition particle (cpSRP) system to reach the thylakoid membrane. Both prokaryotes and eukaryotes possess a cytoplasmic SRP containing a 54-kD protein (SRP54) and an RNA. In chloroplasts, the homolog of SRP54 was found to bind a 43-kD protein (cpSRP43) rather than to an RNA. We cloned the CAO gene, which encodes a protein identified as Arabidopsis cpSRP43. The product of the CAO gene does not resemble any protein in the databases, although it contains motifs that are known to mediate protein-protein interactions. These motifs include ankyrin repeats and chromodomains. Therefore, CAO encodes an SRP component that is unique to plants. Surprisingly, the phenotype of the cpSRP43 mutant (i.e., chaos) differs from that of the Arabidopsis cpSRP54 mutant, suggesting that the functions of the two proteins do not strictly overlap. This difference also suggests that the function of cpSRP43 is most likely restricted to protein targeting into the thylakoid membrane, whereas cpSRP54 may be involved in an additional process(es), such as chloroplast biogenesis, perhaps through chloroplast-ribosomal association with chloroplast ribosomes.
Severe community-acquired pneumonia (2002)

Although several pneumonia severity criteria have been firmly established, the exact definition of severe community-acquired pneumonia (CAP) remains elusive. Mortality from CAP remains high, reaching 50% in some series. The particular role of and spp. in severe CAP has been defined more clearly. Microbial diagnosis in the individual patient remains a difficult task. Despite promising new diagnostic tools, concerns about possible mixed origins preclude a change from the currently advocated broad-spectrum approach of antimicrobial treatment. Although there is some evidence that guidelines may optimize outcomes, their role in limiting the spread of resistance has only recently received attention. Finally, although there are promising data on the use of noninvasive positive pressure ventilation to treat pneumonia in patients without chronic obstructive pulmonary disease, its place in the management of acute respiratory failure remains to be defined in randomized studies.
Resonant Modes in a Maser Interferometer (1961)

A theoretical investigation has been undertaken to study diffraction of electromagnetic waves in Fabry-Perot interferometers when they are used as resonators in optical masers. An electronic digital computer was programmed to compute the electromagnetic field across the mirrors of the interferometer where an initially launched wave is reflected back and forth between the mirrors. It was found that after many reflections a state is reached in which the relative field distribution does not vary from transit to transit and the amplitude of the field decays at an exponential rate. This steady-state field distribution is regarded as a normal mode of the interferometer. Many such normal modes are possible depending upon the initial wave distribution. The lowest-order mode, which has the lowest diffraction loss, has a high intensity at the middle of the mirror and rather low intensities at the edges. Therefore, the diffraction loss is much lower than would be predicted for a uniform plane wave. Curves for field distribution and diffraction loss are given for different mirror geometries and different modes. Since each mode has a characteristic loss and phase shift per transit, a uniform plane wave which can be resolved into many modes cannot, properly speaking, be resonated in an interferometer. In the usual optical interferometers, the resolution is too poor to resolve the individual mode resonances and the uniform plane wave distribution may be maintained approximately. However, in an oscillating maser, the lowest-order mode should dominate if the mirror spacing is correct for resonance. A confocal spherical system has also been investigated and the losses are shown to be orders of magnitude less than for plane mirrors.
In situ Raman spectroscopy for simultaneous monitoring of multiple process parameters in mammalian cell culture bioreactors (2012)

In this study, the application of Raman spectroscopy to the simultaneous quantitative determination of glucose, glutamine, lactate, ammonia, glutamate, total cell density (TCD), and viable cell density (VCD) in a CHO fed-batch process was demonstrated in situ in 3 L and 15 L bioreactors. Spectral preprocessing and partial least squares (PLS) regression were used to correlate spectral data with off-line reference data. Separate PLS calibration models were developed for each analyte at the 3 L laboratory bioreactor scale before assessing its transferability to the same bioprocess conducted at the 15 L pilot scale. PLS calibration models were successfully developed for all analytes bar VCD and transferred to the 15 L scale.
Common pattern of two distinct types of colony-stimulating factor in human tissues and cultured cells. (1980)

Conditioned media prepared from human lung, placenta, peripheral leukocytes, cultured human pancreatic carcinoma cells, and cultured cervical carcinoma cells exhibit a common pattern of two distinct colony-stimulating factors (CSF) separable by isoelectrofocusing. Type I CSF appears polydisperse on isoelectrofocusing, showing multiple peaks with an isoelectric point of 3.6-4.7 and mol wt 50,000. It has a much higher activity in mouse than in human bone marrow, and stimulates the formation of predominately macrophage colonies. Type II CSF has an isoelectric point of 5.7 and mol wt 27,000 and exhibits higher activity in human than in mouse marrow, yielding predominately granulocytic colonies.
Electrical transport in doped multiwalled carbon nanotubes (2001)

The effects of doping, electron coherence, and electron correlation on the transport properties of boron-doped multiwalled carbon nanotubes are studied. Substitutional boron lowers the Fermi level of the tubes and increases the number of participating conduction channels without introducing strong carrier scattering. From 300 to about 50 K, the tubes show metallic behavior with weak electron-phonon coupling. At lower temperatures the resistance increases, and a zero-bias anomaly is observed. The magnetoresistance is now negative indicating the importance of coherent back-scattering processes. The coherence lengths are measured and dephasing is found to involve weakly inelastic electron-electron collisions. The temperature dependence of the resistance as well as the other low temperature observations can be accounted for by one-dimensional weak-localization theory.
A “Smart” Magnetic Resonance Imaging Agent That Reports on Specific Enzymatic Activity (1997)

A noninvasive means to map biological structure is offered by magnetic resonance imaging (MRI). A new class of MRI contrast agents is described where the relaxivity of the complex is modified by the activity of a specific enzyme (β-galactosidase, [Eq. (a)]). This type of agent offers the promise of direct three-dimensional visualization of gene expression in the form of an acquired MR image.
Prevention of third molar development in dog with long pulse diode laser: A preliminary report (2007)

The diode laser may be capable of selectively stopping third molar development and further studies are warranted.
Machine Learning for Outcome Prediction of Acute Ischemic Stroke Post Intra-Arterial Therapy (2014)

We showed promising accuracy of outcome prediction, using supervised machine learning algorithms, with potential for incorporation of larger multicenter datasets, likely further improving prediction. Finally, we propose that a robust machine learning system can potentially optimise the selection process for endovascular versus medical treatment in the management of acute stroke.
Successful Administration of Intranasal Glucagon in the Out-of-Hospital Environment (2012)

We present a case of successful prehospital treatment of hypoglycemia with intranasal (IN) glucagon. Episodes of hypoglycemia can be of varying severity and often requires quick reversal to prevent alteration in mental status or hypoglycemic coma. Glucagon has been shown to be as effective as glucose for the treatment of hypoglycemia. The inability to obtain intravenous (IV) access often impairs delivery of this peptide and is therefore frequently given via the intramuscular (IM) route. Intranasal administration of glucagon has been shown to be as effective as the IV route and may be used for rapid correction of hypoglycemic episodes where IV access is difficult or unavailable and IM administration is undesirable. We describe the first documentation in the peer-reviewed literature of the successful treatment and reversal of an insulin-induced hypoglycemic episode with IN glucagon in the prehospital setting. We also present a review of the literature regarding this novel medication administration route.
Modèle mathématique multi-échelle de l'angiogenèse tumorale et application à l'analyse de l'efficacité de traitements anti-angiogéniques. (2008)

International audience
A context-aware data management system for ubiquitous computing applications (2004)

One of the factors that differentiates ubiquitous computing from traditional distributed computing is context. Context, such as time, location, and situation, allows a system to adapt to the current surroundings in order to facilitate the use of the computational environment. In this paper, we present a file system for ubiquitous computing applications that is context-aware. Context is used to support the types of applications and devices that are found in ubiquitous computing spaces. Novel features of the system include how the view of data adapts to the activity being currently performed and how user data is imported into the local environment. Our system is evaluated as part of a ubiquitous computing infrastructure deployed in a seminar room to investigate issues of performance, scalability, and usability.
Oral Appliances for Snoring and Obstructive Sleep Apnea: A Review (2006)

We conducted an evidence-based review of literature regarding use of oral appliances (OAs) in the treatment of snoring and obstructive sleep apnea syndrome (OSA) from 1995 until the present. Our structured search revealed 141 articles for systematic scrutiny, of which 87 were suitable for inclusion in the evidence base, including 15 Level I to II randomized controlled trials and 5 of these trials with placebo-controlled treatment. The efficacy of OAs was established for controlling OSA in some but not all patients with success (defined as no more than 10 apneas or hypopneas per hour of sleep) achieved in an average of 52% of treated patients. Effects on sleepiness and quality of life were also demonstrated, but improvements in other neurocognitive outcomes were not consistent. The mechanism of OA therapy is related to opening of the upper airway as demonstrated by imaging and physiologic monitoring. Treatment adherence is variable with patients reporting using the appliance a median of 77% of nights at 1 year. Minor adverse effects were frequent whereas major adverse effects were uncommon. Minor tooth movement and small changes in the occlusion developed in some patients after prolonged use, but the long-term dental significance of this is uncertain. In comparison to continuous positive airway pressure (CPAP), OAs are less efficacious in reducing the apnea hypopnea index (AHI), but OAs appear to be used more (at least by self report), and in many studies were preferred over CPAP when the treatments were compared. OAs have also been compared favorably to surgical modification of the upper airway (uvulopalatopharyngoplasty, UPPP). Comparisons between OAs of different designs have produced variable findings. The literature of OA therapy for OSA now provides better evidence for the efficacy of this treatment modality and considerable guidance regarding the frequency of adverse effects and the indications for use in comparison to CPAP and UPPP.
Three-Dimensional Optical Storage Memory (1989)

A novel three-dimensional (3-D) optical memory device is presented that allows fast random access of the information and extremely high bit densities. This device is based on two-photon writing, reading, and erasing of the information in a photochromic material embedded in a polymer matrix. Absorption and emission data show that two-photon writing and reading of information is feasible. The advantages and properties of such a 3-D optical memory are discussed.
Calculations on the Optical Modulation Transfer Function of the Human Eye for White Light (1974)

Modulation transfer functions of the dioptrics of the human eye for white (equal energy distribution) light were calculated for different pupil sizes from experimental data on the aberrations. The largest aberration is the chromatic difference of focus. Since this aberration is well known quantitatively and is subject to small individual differences only, the calculated modulation transfer functions can be considered as representative for human eyes in general. The present calculations, the fundus reflection measurements of the line-spread function by Campbell and Gubisch [1], and the measurements of retinal scattering by Ohzu and Enoch [2] confirm each other. Modulation transfer functions were also calculated for just noticeable focusing errors, and may be informative on the tolerance limit to visual image quality. The calculations have been extended to the special conditions of vision at low luminances.
Expression of Cloned Yeast NADPH-Cytochrome P450 Reductase Gene in Saccharomyces cerevisiae1 (1990)

The NADPH-cytochrome P450 reductase gene isolated from the yeast Saccharomyces cerevisiae [Yabusaki et al., J. Biochem. 103, 1004-1010 (1988)] was expressed on a multi-copy plasmid in the yeast. The transformed yeast cells with the recombinant plasmid carrying the reductase gene with a length of 3 kb produced the corresponding mRNA read from the original transcription initiation site under the control of its own promoter with a maximum length of 300 bp. The reductase content in the transformed cells was 25 times higher than that of the endogenous reductase. When the coding region for the reductase was placed between the alcohol dehydrogenase I gene promoter and the terminator of the expression vector pAAH5, the expression level was 32 times higher than at the endogenous level. These recombinant yeast strains showed enhanced cytochrome c reductase activity with increased cellular reductase levels. A simultaneous expression of yeast P450 reductase with rat P450c or bovine P450(17 alpha) resulted in 25 times or a 5 times increase in the corresponding P450-dependent monooxygenase activity of the recombinant yeast strains, respectively, as compared with that of the yeast cells expressing the corresponding P450 species. These results suggested that the overproduction of yeast P450 reductase with a simultaneous expression of the mammalian P450 species enhanced the P450c- and P450(17 alpha)-dependent monooxygenase activities in the recombinant yeast strains, probably due to the increased frequency of the interaction between yeast P450 reductase and P450c or P450(17 alpha) in the yeast microsomes.
Establishment and characterization of an erythropoietin-dependent subline, UT-7/Epo, derived from human leukemia cell line, UT-7 (1993)

Abstract UT-7 is a human leukemic cell line capable of growing in interleukin-3 (IL-3), granulocyte/macrophage colony-stimulating factor (GM-CSF), or erythropoietin (Epo) (Komatsu et al, Cancer Res 51:341, 1991). To study the effect of Epo on proliferation and differentiation of UT-7, we maintained the UT-7 cell culture for more than 6 months in the presence of Epo. As a result, a subline, UT-7/Epo, was established. The growth of UT-7/Epo could be supported by Epo but not by GM-CSF or IL-3. UT- 7/Epo showed a greater level of heme content and ratio of benzidine- positive staining cells than did UT-7. Butyric acid promoted the synthesis of hemoglobin in UT-7/Epo, but not UT-7. Further, the mRNA concentrations of the c-myb oncogene and GM-CSF receptor beta-subunit were decreased substantially in UT-7/Epo cells. These findings showed that UT-7/Epo cells had progressed further in erythroid development than UT-7 cells, and suggested that long-term culture in Epo had promoted this differentiation. Whereas availability of the Epo receptor (Epo-R) for binding of Epo was reduced in UT-7/Epo cells compared with UT-7 cells, the Epo-R showed a similar affinity for Epo. This observation suggested that change(s) in postreceptor signaling step might be involved in the establishment and maintenance of the UT-7/Epo phenotype.
Response Surface Methodology: Process and Product Optimization Using Designed Experiments (1996)

From the Publisher:
Using a practical approach, it discusses two-level factorial and fractional factorial designs, several aspects of empirical modeling with regression techniques, focusing on response surface methodology, mixture experiments and robust design techniques. Features numerous authentic application examples and problems. Illustrates how computers can be a useful aid in problem solving. Includes a disk containing computer programs for a response surface methodology simulation exercise and concerning mixtures.
A delta F508 mutation in mouse cystic fibrosis transmembrane conductance regulator results in a temperature-sensitive processing defect in vivo. (1996)

The most prevalent mutation (delta F508) in cystic fibrosis patients inhibits maturation and transfer to the plasma membrane of the mutant cystic fibrosis transmembrane conductance regulator (CFTR). We have analyzed the properties of a delta F508 CFTR mouse model, which we described recently. We show that the mRNA levels of mutant CFTR are normal in all tissues examined. Therefore the reduced mRNA levels reported in two similar models may be related to their intronic transcription units. Maturation of mutant CFTR was greatly reduced in freshly excised oviduct, compared with normal. Accumulation of mutant CFTR antigen in the apical region of jejunum crypt enterocytes was not observed, in contrast to normal mice. In cultured gallbladder epithelial cells from delta F508 mice, CFTR chloride channel activity could be detected at only two percent of the normal frequency. However, in mutant cells that were grown at reduced temperature the channel frequency increased to over sixteen percent of the normal level at that temperature. The biophysical characteristics of the mutant channel were not significantly different from normal. In homozygous delta F508 mice we did not observe a significant effect of genetic background on the level of residual chloride channel activity, as determined by the size of the forskolin response in Ussing chamber experiments. Our data show that like its human homologue, mouse delta F508-CFTR is a temperature sensitive processing mutant. The delta F508 mouse is therefore a valid in vivo model of human delta F508-CFTR. It may help us to elucidate the processing pathways of complex membrane proteins. Moreover, it may facilitate the discovery of new approaches towards therapy of cystic fibrosis.
Identification of recurrent and novel mutations in the LDL receptor gene in Spanish patients with familial hypercholesterolemia (1998)

We used the single strand conformation polymorphism (SSCP) method to investigate 13 apparently unrelated Spanish patients with familial hypercholesterolemia (FH) for mutations in the promoter region and the 18 exons and their flanking intron sequences of the low density lipoprotein (LDL) receptor gene. We found 16 aberrant SSCP patterns, and the underlying mutations were characterized by DNA sequencing. Five novel missense mutations, Q71E, C74G, C95R, C281Y and D679E, and one nonsense mutation, Q133X, were identified. We also found six missense mutations, S156L, D200Y, D200G, E256K, T413K and C646Y, and one stop codon mutation, W(-18)X, that were previously described in patients from other populations. A new frameshift mutation, 2085del19, was found in one patient. We also identified three splicing mutations; two of them are novel mutations, 1706-10G→A and 2390-1G→A, and the other one has been reported recently, 313+1G→C. Four patients were found to carry two different mutations in the same allele: Q71E and 313+1G→C; C95R and D679E; W(-18)X and E256K, and C281Y and 1706-10G→A. Our results demonstrate that there is a broad spectrum of mutations in the LDL receptor gene in the Spanish population. Hum Mutat 11:413, 1998. © 1998 Wiley-Liss, Inc.
Physician Variation in Diagnostic Testing for Low Back Pain. Who You See Is What You Get (1994)

There is little consensus, either within or among specialties, on the use of diagnostic tests for patients with back pain. Thus, the diagnostic evaluation depends heavily on the individual physician and his or her specialty, and not just the patient's symptoms and findings. Furthermore, many physicians may be ordering imaging studies too early and for patients who do not have the appropriate clinical indications. These results suggest a need for additional clinical guidelines as well as better adherence to existing guidelines.
Primary structure of the low molecular weight nucleic acid-binding proteins of murine leukemia viruses. (1981)

Murine leukemia viruses contain a low molecular weight basic protein, designated p10, which binds to single-stranded nucleic acids. The complete amino acid sequence of p10 from the Rauscher strain of virus has been determined. The partial amino acid sequences of p10s from Moloney, Friend, AKR, Gross, radiation leukemia, and BALB/2 viral strains have also been determined using microsequencing techniques. Rauscher p10 is composed of 56 amino acid residues; the other p10s are similar in size but differ from Rauscher by a few conservative amino acid substitutions. The structure of Rauscher p10 was compared to the structure of a functionally homologous protein from Rous avian sarcoma virus. The comparison revealed regions of amino acid sequence homologies which indicate a phylogenetic relationship between the murine and avian viral strains. The analyses revealed a periodic placement of three Cys residues and a Gly-His sequence. A structure involving these residues is found once in the murine protein and twice in the avian protein. A similar structure is seen in the single stranded nucleic acid binding protein of bacteriophage T4. However, in the latter case, the order of amino acid residues is inverted.
Immunogenicity of Recombinant Adenovirus-Respiratory Syncytial Virus Vaccines with Adenovirus Types 4, 5, and 7 Vectors in Dogs and a Chimpanzee (1992)

Recombinant adenovirus type 4, 5, and 7 expressing the fusion glycoprotein (F) gene, the attachment glycoprotein (G) gene, or both F and G genes of respiratory syncytial virus (RSV) was constructed. Intratracheal immunization of dogs with Ad7F induced moderate titers of RSV-neutralizing antibodies. After booster immunization with Ad4F, the dogs developed high titers of RSV-specific antibody. Subsequently, three two-dose vaccination regimens, Ad4F/Ad5F, Ad7G/Ad4G, and Ad7FG/Ad4FG, were compared with Ad7F/Ad4F for immunogenicity and protective efficacy. The results indicated that Ad4F/Ad5F was equal or greater in immunogenicity to Ad7F/Ad4F, but Ad7G/Ad4G and Ad7FG/Ad4FG were less effective than Ad7F/Ad4F in inducing RSV-neutralizing antibody. All vaccination regimens completely protected the lungs of dogs from RSV infection. A chimpanzee was sequentially immunized orally with Ad7F, Ad4F, and Ad5F. A low-level antibody response to RSV was induced after the primary immunization, but no significant increases were observed after booster immunizations.