gebhart/patrepeval huggingface dataset:
Endothelial Progenitor Cell-Derived Microvesicles Improve Neovascularization in a Murine Model of Hindlimb Ischemia (2012)

Paracrine mediators released from endothelial progenitor cells (EPCs) have been implicated in neoangiogenesis following ischemia. Recently, we demonstrated that microvesicles (MVs) derived from EPCs are able to activate an angiogenic program in quiescent endothelial cells by a horizontal transfer of RNA. In this study we aim to investigate whether EPC-derived MVs are able to induce neoangiogenesis and to enhance recovery in a murine model of hindlimb ischemia. Hindlimb ischemia was induced in severe combined immunodeficient (SCID) mice by ligation and resection of the left femoral artery and mice were treated with EPC-derived MVs (MVs), RNase-inactivated MVs (RnaseMVs), fibroblast-derived MVs or vehicle alone as control (CTL). Since MVs contained the angiogenic miR-126 and miR-296, we evaluated whether microRNAs may account for the angiogenic activities by treating mice with MVs obtained from DICER-knock-down EPC (DICER-MVs). The limb perfusion evaluated by laserdoppler analysis demonstrated that MVs significantly enhanced perfusion in respect to CTL (0.50±0.08 vs 0.39±0.03, p<0.05). After 7 days, immunohistochemical analyses on the gastrocnemius muscle of the ischemic hindlimb showed that MVs but not fibroblast-MVs significantly increased the capillary density in respect to CTL (MVs vs CTL: 24.7±10.3 vs 13.5±6, p<0.0001) and (fibroblast-MVs vs CTL: 10.2±3.4 vs 13.5±6, ns); RNaseMVs and DICER-MVs significantly reduced the effect of MVs (RNaseMVs vs CTL: 15.7±4.1 vs 13.5±6, ns) (MVs vs DICER-MVs 24.7±10.3 vs 18.1±5.8, p <0.05), suggesting a role of RNAs shuttled by MVs. Morphometric analysis confirmed that MVs enhanced limb perfusion and reduced injury. The results of the present study indicate that treatment with EPC-derived MVs improves neovascularization and favors regeneration in severe hindlimb ischemia induced in SCID mice. This suggests a possible use of EPCs-derived MVs for treatment of peripheral arterial disease.
Effect of Eye Pigmentation on Transscleral Drug Delivery (2008)

Transscleral retinal and vitreal drug delivery of lipophilic celecoxib is significantly lower in pigmented rats than in albino rats. This difference may be attributable to significant binding of celecoxib to melanin and its accumulation/retention in the melanin-rich choroid-RPE of pigmented rats. The hindrance of retinal and vitreal drug delivery by the choroid-RPE in pigmented rats is also true of sustained-release microparticle systems.
Haplodiploidization of maize (Zea mays L) through induced gynogenesis assisted by glossy markers and its use in breeding (1997)

Reliable detection of haploids at the seedling stage is a prerequisite for the use of haplodiploidization through in situ gynogenesis in maize breeding. The use of recessive alleles of glossy genes as markers makes this detection possible in various materials. Two synthetics with a wide genetic basis: flint glossy1 CGT and dent glossy6 DGT were studied. After pollination with the FIGH 1 inducer, they produced haploid seedlings at a rate of 0.64 and 0.93%, respectively. The rate is increased (0.94% on average) for the CGT (C 0 ) synthetic after selfing, in S 1 proge- nies. The distribution of 203 S 1 families showed a significant deviation in comparison with a Poisson distribution. The DGT synthetic showed an increase in induction rate when a new synthetic was formed with doubled haploid (DH) lines, suggesting that 'inductibility' may have some genetic effects. No spontaneous doubling was shown by the hap- loid seedlings of either synthetic. Colchicine treatment allowed a recovery of male fertility in 30 to 60% of detected haploids according to the experiments. The progeny recovery rate was influenced by the genetic basis, as the DGT synthetic gave better results than the CGT synthetic. The growing period also had an influence upon the recovery of fertile ears. Hybrids produced from heterotic DH lines showed an agronomic potential similar to standard hybrids. It is concluded that in situ gynogenesis assisted by the use of glossy markers can now be used in maize breeding. maize breeding / in situ haploids / diploidization / colchicine / glossy Rsum &mdash; Haplodiplodisation du mas (Zea mays L) par gynogense induite assiste par marqueurs glossy et son application en slection. L'utilisation effective de l'haplodiplodisation par gynogense in situ en slection du mas ncessite une dtection fiable des haplodes au stade plantule. Le marquage gnotypique par des allles rcessifs des gnes glossy permet cette dtection. Deux synthtiques base large: CGT (corne glossyl tardive), DGT (den- te glossy6 tardive), ont t tudies. Pollinises par l'inducteur FIGH 1, elles ont produit des plantes haplodes des taux respectifs de : 0,64 et 0,93 %. Pour la synthtique CGT (C 0 ), ce taux est augment (0,94 % en moyenne) lorsque l'on passe de la premire gnration aux descendances S 1 . L'analyse de la distribution de l'induction de 203 S 1 de CGT montre une dviation significative par rapport une distribution de Poisson. Dans la population DGT, le taux d'induction de plantes haplodes s'est accru par la constitution d'une synthtique partir de lignes haplodes dou- bles (HD). Cela suggre des effets gntiques pour l'inductibilit. Les plantules haplodes provenant de CGT et DGT n'ont pas prsent de diplodisation spontane. Le traitement la colchicine permet la restauration de la fertilit mle dans 30 60 % des cas selon les essais. Le taux de descendances diplodes fertiles obtenues par rapport au
Efficient universal lossless data compression algorithms based on a greedy sequential grammar transform-part two: with context models (2003)

For pt. I see ibid., vol.46, p.755-88 (2000). The concept of context-free grammar (CFG)-based coding is extended to the case of countable-context models, yielding context-dependent grammar (CDG)-based coding. Given a countable-context model, a greedy CDG transform is proposed. Based on this greedy CDG transform, two universal lossless data compression algorithms, an improved sequential context-dependent algorithm and a hierarchical context-dependent algorithm, are then developed. It is shown that these algorithms are all universal in the sense that they can achieve asymptotically the entropy rate of any stationary, ergodic source with a finite alphabet. Moreover, it is proved that these algorithms' worst case redundancies among all individual sequences of length n from a finite alphabet are upper-bounded by d log log n/log n, as long as the number of distinct contexts grows with the sequence length n in the order of O(n/sup a/), where 0 < /spl alpha/ < 1 and d are positive constants. It is further shown that for some nonstationary sources, the proposed context-dependent algorithms can achieve better expected redundancies than any existing CFG-based codes, including the Lempel-Ziv (1978) algorithm, the multilevel pattern matching algorithm, and the context-free algorithms in Part I of this series of papers.
Tethered Neuraminidase Inhibitors That Bind an Influenza Virus: A First Step Towards a Diagnostic Method for Influenza (2003)

Grippe „eingefangen“: Durch 7-Funktionalisierung von Zanamivir (rot), einem Neuraminidase-Inhibitor zur Behandlung von Influenza, werden Derivate mit unverändert hoher Affinität für das Enzym erhalten. Konjugiert an Biotin (grün) oder Mikrokügelchen können sie zur Immobilisierung von Influenza-Viren genutzt werden.
Increased ionizing radiation sensitivity and genomic instability in the absence of histone H2AX (2002)

In mammalian cells, DNA double-strand breaks (DSBs) cause rapid phosphorylation of the H2AX core histone variant (to form gamma-H2AX) in megabase chromatin domains flanking sites of DNA damage. To investigate the role of H2AX in mammalian cells, we generated H2AX-deficient (H2AX(Delta)/Delta) mouse embryonic stem (ES) cells. H2AX(Delta)/Delta ES cells are viable. However, they are highly sensitive to ionizing radiation (IR) and exhibit elevated levels of spontaneous and IR-induced genomic instability. Notably, H2AX is not required for NHEJ per se because H2AX(Delta)/Delta ES cells support normal levels and fidelity of V(D)J recombination in transient assays and also support lymphocyte development in vivo. However, H2AX(Delta)/Delta ES cells exhibit altered IR-induced BRCA1 focus formation. Our findings indicate that H2AX function is essential for mammalian DNA repair and genomic stability.
Effects of inducers of differentiation on protein kinase C and CMP-N-acetylneuraminic acid:lactosylceramide sialyltransferase activities of HL-60 leukemia cells. (1989)

Exposure of HL-60 leukemia cells to either 12-O-tetradecanoylphorbol-13-acetate (TPA), dimethylsulfoxide (DMSO), exogenous gangliosides GM3, GM1, or bovine brain ganglioside mixture (BBG) resulted in a marked inhibition of the growth of cells. The order of the inhibitory potency was TPA greater than GM3 greater than DMSO greater than BBG greater than GM1. In contrast, sulfatides were without effect on cellular replication. Treatment of HL-60 cells with TPA or GM3 induced differentiation along the monocyte/macrophage lineage, while treatment with DMSO induced maturation along the granulocytic pathway. These effects were accompanied by more than a twofold increase in protein kinase C (PKC) activity. In contrast, treatment with GM1, BBG, or sulfatides caused only a relatively small increase in PKC activity. The activity of CMP-N-acetylneuraminic acid:lactosylceramide sialyltransferase (ST1), a key enzyme for membrane gangliosides synthesis, in HL-60 cells was also influenced by the exposure to TPA, GM3, DMSO, GM1, or sulfatides. The inducers of differentiation, TPA and DMSO, caused an increase in ST1 activity, whereas GM3, which also induced cellular differentiation, inhibited ST1 activity, perhaps through the action of end-product inhibition. The non-inducers of differentiation, GM1 and sulfatides, also increased the activity of ST1, but to a much lesser extent. The findings suggest that the direct or indirect modulation of PKC activity by some of these agents may be involved, at least in part, in the regulation of cellular growth and differentiation. Furthermore, it is conceivable that differences in PKC activity may be responsible for the changes in ST1 activity associated with cell differentiation and proliferation.
Monoclonal antibody purification using cationic polyelectrolytes: An alternative to column chromatography (2010)

The potential of cationic polyelectrolytes to precipitate host cell and process related impurities was investigated, to replace one or more chromatography steps in monoclonal antibody purification. The impact of antibody isoelectric point, solution properties (pH and ionic strength), and polyelectrolyte properties (structure, molecular weight and pK(a)) on the degree of precipitation was studied. At neutral pH, increasing solution ionic strength impeded the ionic interaction between the polyelectrolyte and impurities, reducing impurity precipitation. Increasing polyelectrolyte molecular weight and pK(a) enabled precipitation of impurities at higher ionic strength. PoIy(arginine) was selected as the preferred polyelectrolyte in unconditioned cell culture fluid. PoIy(arginine) precipitation achieved consistent host cell protein clearance and antibody recovery for multiple antibodies across a wider range of polyelectrolyte concentrations. Poly(arginine) precipitation was evaluated as a flocculant and as a functional replacement for anion exchange chromatography in an antibody purification process. Upstream treatment of cell culture fluid with poly(arginine) resulted in flocculation of solids (cells and cell debris), and antibody recovery and impurity clearance (host cell proteins, DNA and insulin) comparable to the downstream anion exchange chromatography step.
Entry of Shigella flexneri into HeLa cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulation (1987)

The enteroinvasive bacterium Shigella flexneri expresses a plasmid-mediated capacity to penetrate into nonphagocytic cells. By using 7-nitrobenz-2-oxa-1,3-diazole-phallacidin (NBD-phallacidin), a fluorescent dye which specifically stains microfilaments, we observed condensations of filamentous actin underneath the plasma membrane of HeLa cells which interacted with the invasive isolate M90T. As demonstrated by indirect immunofluorescence with the antimyosin monoclonal antibody CC-212, myosin accumulated at the same sites. The entry process could be synchronized by using strain SC301, a pIL22 transformant of M90T. pIL22, a recombinant plasmid encoding the Escherichia coli afimbrial adhesin AFA I, rendered shigellae highly adherent to HeLa cells. Using such a system, we demonstrated that the occurrence of bacterial penetration and the appearance of structures brightly stained by NBD-phallacidin were simultaneous events. Such microfilamentous structures resulted from de novo polymerization of the monomeric actin pool in a DNase I inhibition assay, as shown by measurement of the monomeric versus total actin content of infected HeLa cells. These data provide direct evidence that the penetration of S. flexneri into HeLa cells occurs through a mechanism similar to phagocytosis by professional phagocytes.
[Study of MSCs in vitro cultured on demineralized bone matrix of mongrel]. (2007)

DBM has satisfactory biocompatibility.
Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding. (1993)

The integrase protein of human immunodeficiency virus type 1 carries out a set of polynucleotidyl transfer reactions that result in the covalent attachment of the retroviral cDNA to host DNA. We have analyzed the activities of a set of deletion derivatives of the integrase protein. The analysis reveals that a central domain of only 137 amino acids is sufficient in vitro to catalyze a subset of the reactions carried out by the complete protein. This polypeptide contains an amino acid sequence motif, Asp-Xaa39-58-Asp-Xaa35-Glu (DX39-58DX35E, where X and the subscript indicate the intervening amino acids between the invariant acidic residues), that is found in the integrases of retroviruses and retrotransposons and also the transposase proteins of some bacterial transposable elements. We also find that the integrase protein can bind Zn2+, and the histidine and cysteine residues of another conserved motif (HX3-7HX23-32CX2C) are required for efficient Zn2+ binding. The activities displayed by deletion mutants suggest to us possible functions for the various parts of integrase.
Activation of α1B-adrenoceptors alleviates ischemia/reperfusion injury by limitation of mitochondrial Ca2+ overload in cardiomyocytes (2007)

These findings indicate that phenylephrine-induced protection on [Ca(2+)](m) homeostasis is mediated by selective activation of alpha(1B)-AR via mitoK(ATP) channel opening and PKCepsilon activation. Mitochondrial function appears to be a determinant of [Ca(2+)](c) and contractile function during I/R injury.
Sleepiness in Patients with Moderate to Severe Sleep-Disordered Breathing (2005)

In this community-based cohort, subjective sleepiness is absent in many individuals with significant sleep-disordered breathing. Comorbid conditions, including respiratory disease, sleep restriction, insomnia, and nocturnal leg complaints, are important risk factors for sleepiness in individuals with moderate to severe sleep-disordered breathing.
ARCES: an echelle spectrograph for the Astrophysical Research Consortium (ARC) 3.5m telescope (2003)

A new echelle spectrograph was commissioned in 1999 for the ARC 3.5 meter telescope. The key features of the instrument are that it has a resolution of 9 km/sec, limited by the pixel size of the CCD; has no moving parts behind the slit during observation; provides complete spectral coverage from 3200A to 10000A, limited by the prism cross disperser material on the blue side and by the CCD sensitivity on the red side; provides blazeless spectra; achieves S/N&gt;3000; and is remotely operable. The instrument is being used for studies of abundances in stars and for a large survey of diffuse interstellar bands.
Status of ASTM specifications for gasoline-oxygenate blends (1985)

Subcommittee A of the American Society for Testing and Materials Committee D-2 began studying the suitability of Specification D 439 for gasoline-oxygenate blends in December 1978. An information document on gasohol was published November 1980 and revised March 1981. A Proposed Specification for Gasohol and Leaded Gasohol was published May 1981. A standard specification for both gasoline and gasoline-oxygenate blends received too many negative votes. A proposed specification for both gasoline and gasoline-oxygenate blends is now under development. Specification D 439 will be modified to recognize the existence of gasoline-oxygenate blends and will refer to the proposed specification for applicability of test methods and additional requirements for blends.
Wheat Loads and Vertical Pressure Distribution in a Full-scale Bin Part I Filling (1994)

Flat-bottom cylindrical grain bins are subjected to unique loads and pressures during the filling process. The vertical and lateral loads imposed on a bin wall and the vertical load on a bin floor by wheat during filling were measured. The radial distribution of vertical pressure during filling was also measured. The vertical pressure was determined to be dependent on the radial location and was not uniformly distributed. The load distribution in a bin with an H/D ratio of 3 was 83% on the floor and 17% on the walls. Three widely used prediction equations of Janssen, Reimbert, and Walker were compared to the measured loads and pressures and were determined to have similar shapes but different parameters for a best fit to observed data.
Extended depth of field imaging for high speed cell analysis (2007)

The use of EDF techniques may significantly enhance the quantitation of cell imagery, particularly in applications such as FISH, where small discrete signals must be detected over a wide focal range within the cell.
EFFICIENT METHODS FOR THE SYNTHESIS OF [2-15N]GUANOSINE AND 2′-DEOXY[2-15N]GUANOSINE DERIVATIVES (2001)

The nucleophilic addition-elimination reaction of 2',3',5'-tri-O-acetyl-2-fluoro-O6-[2-(4-nitrophenyl)ethyl]inosine (8) with [15N]benzylamine in the presence of triethylamine afforded the N2-benzyl[2-15N]guanosine derivative (13) in a high yield, which was further converted into the N2-benzoyl[2-15N] guanosine derivative by treatment with ruthenium trichloride and tetrabutylammonium periodate. A similar sequence of reactions of 2',3',5'-tri-O-acetyl-2-fluoro-06-[2-(methylthio)ethyl]inosine (9) and the 6-chloro-2-fluoro-9-(beta-D-ribofuranosyl)-9H-purine derivative (11), which were respectively prepared from guanosine, with potassium [15N]phthalimide afforded the N2-phthaloyl [2-15N]guanosine derivative (15; 62%) and 9-(2,3,5-tri-O-acetyl-beta-D-ribofuranosyl)-6-chloro-2-[15N]phthalimido-9H-purine (17; 64%), respectively. Compounds 15 and 17 were then efficiently converted into 2',3',5'-tri-O-acetyl [2-15N]guanosine. The corresponding 2'-deoxy derivatives (16 and 18) were also synthesized through similar procedures.
Linkage analysis of schizophrenia with five dopamine receptor genes in nine pedigrees. (1993)

Alterations in dopamine neurotransmission have been strongly implicated in the pathogenesis of schizophrenia for nearly 2 decades. Recently, the genes for five dopamine receptors have been cloned and characterized, and genetic and physical map information has become available. Using these five loci as candidate genes, we have tested for genetic linkage to schizophrenia in nine multigenerational families which include multiple affected individuals. In addition to testing conservative disease models, we have used a neurophysiological indicator variable, the P50 auditory evoked response. Deficits in gating of the P50 response have been shown to segregate with schizophrenia in this sample and may identify carriers of gene(s) predisposing for schizophrenia. Linkage results were consistently negative, indicating that a defect at any of the actual receptor sites is unlikely to be a major contributor to schizophrenia in the nine families studied.
Studies on the Cytophilic Properties of Human β2‐Microglobulin (1979)

The mechanism by which exogenously added beta2m binds to lymphoid cells has been explored. In the mouse it has been shown that beta2m remains associated with plasma membrane macromolecules following solubilization with NP-40 and that approximately 25-30% of the binding could be accounted for by binding to H-2 antigens. No binding to mouse immunoglobulin or Ia antigens could be detected. The sites for binding of the remainder of the cell-bound beta2m were not determined. Whereas normal human lymphocytes showed little or no capacity to bind exogenously added beta2m, it was found that phytohaemagglutinin (PHA)-stimulated cells could bind beta2m. This binding occurred optimally 2 days after PHA stimulation. Approximately half of the binding could be accounted for by binding to HLA antigens. The possible significance of these findings with respect to cellular interactions involving major histocompatibility complex gene products in the immune response is discussed.
A Start for Implementing ORCID in the Swedish Research Information Infrastructure (2013)

Texten presenterar i korthet det projekt kring författaridentifikatorer och publikationsdatabaser som Kungliga biblioteket finansierade under 2012 under projektledning av Stockholms universitetsbibliotek med deltagande av biblioteken vid Chalmers, Karolinska Institutet samt Malmö högskola.
Methionine requirement and replacement by homocysteine in tissue cultures of selected rodent and human malignant and normal cells. (1978)

In this paper we discuss the beauty of and in organizations. The field of aesthetics in organization studies largely deals with the aesthetics of material artifacts in or around\norganizations or produced by them. We are interested in the intrinsic beauty of organizations and their work processes and in the possible relation between such beauty and performance. We are also interested in ways to design, develop and sustain the beauty of and in organizations. Literature in this direction is still rather scarce. This\nconceptual paper intends to contribute to a definition of a research agenda for developing aesthetic knowledge that can be used to design, create and sustain beautiful organizations.
Measurement of Insulin Absorption and Insulin Action (2004)

For the practical implementation of every type of insulin therapy it is necessary to know both the time course of action of therapeutically used short- and long-acting insulin preparations and the factors influencing such time-action profiles. The only reliable way to obtain the required quantitative information about the pharmacokinetic and glucodynamic properties of insulin preparations has been the use of the euglycemic glucose clamp technique. The first studies with each new insulin formulation or insulin application technique should be performed with healthy subjects in order to have the most comparable study conditions. Thereafter, results from such clinical-experimental studies should be verified in similar studies with patients with diabetes. Earlier investigational approaches, which either had been limited to the determination of the pharmacokinetic properties of insulin preparations or had used the quantitative decrease of the blood glucose level as a measure of the pharmacodynamic properties, do not provide valid quantitative results. The proposed glucose clamp technique makes possible the quantitative study of the pharmacokinetic and pharmacodynamic properties of insulin preparations under comparative and reproducible conditions.