gebhart/patrepeval huggingface dataset:
A Recombinant E1-Deleted Canine Adenoviral Vector Capable of Transduction and Expression of a Transgene in Human-Derived Cells and In Vivo (1997)

Human adenovirus (HAV) serotypes 2 and 5 are commonly used as vector backbones for adenovirus-mediated gene transfer. However, HAVs were chosen as a backbone for the vectors for historical reasons and have a number of significant disadvantages when used as a shuttle for gene transfer in humans. As an initial trial to circumvent some of the shortcomings of HAV vectors, we have produced an E1-deleted canine adenovirus type 2 (CAV-2) vector for gene transfer. Initially, we demonstrated that CAV-2 undergoes an abortive viral cycle in a wide range of human-derived cell lines. Second, we assayed human sera containing HAV-5 neutralizing antibodies for their ability to inhibit CAV-2-induced plaques on permissive cells. In the cohort tested, our data demonstrate that the humoral response directed against HAV-5 does not inhibit CAV-2 plaque formation in the majority of cases. Canine cell lines expressing the E1 region of CAV-2 were generated and characterized. A recombinant CAV vector (CAVRSVbetagal) deleted in the E1 region and harboring lacZ was constructed. We show that CAVRSVbetagal is able to transduce and direct expression of the transgene in vitro in a variety of mammalian cells, most notably primary human-derived cells. In addition, gene transfer is demonstrated in vivo using chick embryos.
Synovial tissue protease gene expression and joint erosions in early rheumatoid arthritis (2001)

The detection of MMP-1, CB, and CL in the synovium soon after the onset of symptoms highlights the potential for early joint destruction in patients with RA. High levels of MMP-1 mRNA expression in the lining layer distinguished patients with more rapidly progressive erosive disease. This is the first study to demonstrate features of early synovial pathophysiology that may identify patients at increased risk of developing new joint erosions.
Ultrasonic Rheo-Diecasting of A383 Aluminum Alloy (2016)

In this study, un-refined A383 aluminum alloy was cast at different temperatures using ultrasonic melt treatment. The liquid alloy was treated by ultrasonic vibrations in the crucible and/or in the shot sleeve of a pressure diecasting machine. The treatment temperature extended to the semisolid temperature range. The UST melt could be injected into the die cavity while being in the semisolid state which is known as rheodiecasting. The results showed that ultrasonic treatment resulted in finer microstructure, globular Fe-intermetallic particles and partially modified eutectic Si. For samples solidified in shot sleeve: Fe-intermetallics become compacted with UST at all positions of ingot. Si particles are compacted in less acicular form near to horn and acicular at other positions. For the rheo-diecasting experiments, with UST treatment in the crucible and die casting, at 600-588 C and 588-578C, Fe-intermetallics were observed in compact form, and Si particles were highly modified.
Evidence for two loci regulating total serum IgE levels (1994)

Genetic studies of total serum IgE levels are of interest since high IgE levels correlate with clinical expression of allergy and asthma. Previous analysis on data from 92 Dutch families ascertained through a parent with asthma showed evidence for recessive inheritance of high IgE levels with linkage to 5q. Since a significant polygenic component was present, two locus segregation analyses were performed using PAP on the same data to determine whether a portion of the polygenic component is due to a second major locus. Two locus models have been tested which include various two locus epistatic and heterogenic Mendelian models and an environmental model (equal transmission probability). Results revealed that (1) two locus Mendelian models fit the data significantly better than a two locus environmental model (P<0.001), and (2) an epistatic two locus Mendelian model significantly fit the data better than the previous one locus recessive model ({chi}{sup 2} = 12.67, df = 5, P<0.05), and is the most parsimonious and best model among all the models tested. In this model, the mean for genotypes AAbb and aaBB were constrained to be equal (estimated mean IgE = 110 I.U.), the mean for genotypes aaBb, Aabb, and aabb were constrained tomore » be equal (437 I.U.), and the mean for other genotypes are at normal values (<42 I.U.). The first locus (locus A) accounts for 45% of the total variation of IgE values, the second locus (locus B), 18%, while the two loci together, 79%. The recombination suggested that there is a second major locus (probably unlinked to the locus on 5q) and that the two loci work epistically in regulating total serum IgE levels. Further studies are needed to map this locus.« less
Highly efficient editing of the β-globin gene in patient-derived hematopoietic stem and progenitor cells to treat sickle cell disease (2019)

Sickle cell disease (SCD) is a monogenic disorder that affects millions worldwide. Allogeneic hematopoietic stem cell transplantation is the only available cure. Here, we demonstrate the use of CRISPR/Cas9 and a short single-stranded oligonucleotide template to correct the sickle mutation in the β-globin gene in hematopoietic stem and progenitor cells (HSPCs) from peripheral blood or bone marrow of patients with SCD, with 24.5 ± 7.6% efficiency without selection. Erythrocytes derived from gene-edited cells showed a marked reduction of sickle cells, with the level of normal hemoglobin (HbA) increased to 25.3 ± 13.9%. Gene-corrected SCD HSPCs retained the ability to engraft when transplanted into non-obese diabetic (NOD)-SCID-gamma (NSG) mice with detectable levels of gene correction 16-19 weeks post-transplantation. We show that, by using a high-fidelity SpyCas9 that maintained the same level of on-target gene modification, the off-target effects including chromosomal rearrangements were significantly reduced. Taken together, our results demonstrate efficient gene correction of the sickle mutation in both peripheral blood and bone marrow-derived SCD HSPCs, a significant reduction in sickling of red blood cells, engraftment of gene-edited SCD HSPCs in vivo and the importance of reducing off-target effects; all are essential for moving genome editing based SCD treatment into clinical practice.
A Labile Phosphodiester Bond at the Ligation Junction in a Circular Intervening Sequence RNA (1984)

The excised intervening sequence of the Tetrahymena ribosomal RNA precursor mediates its own covalent cyclization in the absence of any protein. The circular molecule undergoes slow reopening at a single phosphodiester bond, the one that was formed during cyclization. The resulting linear molecule has 5'-phosphate and 3'-hydroxyl termini; these are unusual products for RNA hydrolysis but are typical of the other reactions mediated by this molecule. The reopened circle retains cleavage-ligation activity, as evidenced by its ability to undergo another round of cyclization and reopening. The finding that an RNA molecule can be folded so that a specific phosphate can be strained or activated helps to explain how the activation energy is lowered for RNA self-splicing. The proposed mechanisms may be relevant to several other RNA cleavage reactions that are RNA-mediated.
Globe: a wide area distributed system (1999)

The authors present an object-based framework for developing wide-area distributed applications. The World Wide Web's current performance problems illustrate the benefit of encapsulating state, operations, and implementation strategies on a per-object basis. The authors describe how distributed objects can implement worldwide scalable Web documents.
Dense ceramic membranes for methane conversion (1996)

This report focuses on a mechanism for oxygen transport through mixed- oxide conductors as used in dense ceramic membrane reactors for the partial oxidation of methane to syngas (CO and H{sub 2}). The in-situ separation of O{sub 2} from air by the membrane reactor saves the costly cryogenic separation step that is required in conventional syngas production. The mixed oxide of choice is SrCo{sub 0.5}FeO{sub x}, which exhibits high oxygen permeability and has been shown in previous studies to possess high stability in both oxidizing and reducing conditions; in addition, it can be readily formed into reactor configurations such as tubes. An understanding of the electrical properties and the defect dynamics in this material is essential and will help us to find the optimal operating conditions for the conversion reactor. In this paper, we discuss the conductivities of the SrFeCo{sub 0.5}O{sub x} system that are dependent on temperature and partial pressure of oxygen. Based on the experimental results, a defect model is proposed to explain the electrical properties of this system. The oxygen permeability of SrFeCo{sub 0.5}O{sub x} is estimated by using conductivity data and is compared with that obtained from methane conversion reaction.
II. Field trials on kiwifruit and Japanese plums (1980)

Abstract Pollen suspended in aqueous media was sprayed on to Japanese plum and kiwifruit flowers to improve fruit set and fruit size. Supplementary pollination of Black Doris plums using 0.25 and 2.5 mg pollen per ml of medium set as many flowers as hand‐pollination using dry pollen but similar experiments on Mariposa plums using Doris pollen were not effective. Kiwifruit pollen applied at 1.0 mg per ml suspension medium to Hayward kiwifruit flowers increased fruit set and fruit size, particularly when pollination by honey bees was inadequate. Lower or higher concentrations were not as effective as 1.0 mg per ml. The use of supplementary pollination in commercial orchards is discussed.
Identification of PRG1, A Novel Progestin-Responsive Gene with Sequence Homology to 6-Phosphofructo-2-Kinase/Fructose- 2,6-Bisphosphatase (1997)

To define early molecular targets of progestin action, the differential display technique was used to identify genes with altered levels of expression in T-47D breast cancer cells treated with the synthetic progestin ORG 2058 for 3 h. PRG1 was first isolated as a 200-bp cDNA clone and its progestin regulation confirmed by Northern analysis. Cloning of the complete coding region of PRG1 revealed that it shared a high degree of amino acid sequence identity with isoforms of the enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from several tissues and species. Expression of PRG1 mRNA was observed in several normal breast epithelial and breast cancer cell lines and in a variety of human tissues, with highest expression in the breast, aorta, and brain. In T-47D cells, PRG1 mRNA was rapidly and transiently induced by progestins, expression peaking between 2 and 4 h and returning to control levels by 12 h. Progestin-induced increases in PRG1 mRNA were inhibited by the progestin antagonist RU 486 and occurred via the progesterone receptor. Progestin induction of PRG1 mRNA was also inhibited by actinomycin D but not by cycloheximide. PRG1 is therefore a novel human gene that is directly regulated by progestins via the progesterone receptor.
Pteridines CVIII Reactions of 6, 7-Dichloro-I, 3-dimethyllumazine with Sulfur-Nucleophiles (1996)

Summary 6,7-Dichloro-1,3-dimethyllumazine (1) and various 6-chloro-7-O-, S- and N-subst.-1,3-dimethyllumazines (2-7) react under relatively mild reaction condiditions with S-nucleophiles to the corresponding 6-mercapto derivatives 8-16. Treatment of 1 with sodium benzylmercaptide at room temperature led to di-( 6- chloro-1,3-dimethyllumazine-7-yl)sulfide (17) whereas thiourea caused transformation to the corresponding 7,7-disulfide 18. 7-Mercapto-1,3-dimethyl-6(5H)lumazinethione (12) can be modified widely by alkylations to open-chain (21-23, 27-29) or cyclic 6,7-bis-alkylthio derivatives (24-26) and acylations with phosgene and thiophosgene, respectivly, gave the derivatives 36 and 37 of the new 1 ,3-dithiolo[ 4,5-g]pteridine ring system. Ribosylation of 12 afforded a 6,7-bis-S-riboside (40) in high yield.
In vitro neutralization of Chlamydia trachomatis by monovalent Fab antibody specific to the major outer membrane protein (1991)

Monovalent Fab antibodies to serovar- and subspecies-specific epitopes of the major outer membrane protein (MOMP) of Chlamydia trachomatis neutralized infectivity for hamster kidney cells by preventing chlamydial attachment. These findings exclude the aggregation of chlamydiae as a mechanism of anti-MOMP neutralization and provide additional evidence in support of the MOMP as a chlamydial adhesin.
Phosphorylated p-70S6K predicts tamoxifen resistance in postmenopausal breast cancer patients randomized between adjuvant tamoxifen versus no systemic treatment (2014)

Patients whose tumor expresses p-p70S6K, as a marker of downstream PI3K and/or MAPK pathway activation, have a favorable prognosis, but do not benefit from adjuvant tamoxifen. A potential benefit from inhibitors of the PI3K/Akt/mTOR pathway in these patients needs to be further explored.
Symptomatic and pathophysiological observations in a modified animal model of allergic rhinitis. (2005)

There are many animal models for studying allergic rhinitis. However, they either need a too long establishment period or fail to show significant late allergic responses. In the model described in this paper, guinea pigs were sensitized and challenged intranasally with ovalbumin according to a modified protocol. As controls, antihistamine-treatment and non-sensitized, non-treatment groups were studied in parallel. Early and late symptoms, passive cutaneous anaphylaxis (PCA) reaction, nitric oxide synthase (NOS) immunoreactivities, pathological changes in nasal mucosa and nasal lavage fluid (NLF), and histamine, TXB2 and p-LTs levels in NLF were evaluated. In contrast to the control groups, the model group exhibited typical symptoms, including late phase nasal blockage, and increased levels of IgG1 and IgE. Considerable eosinophil infiltration and eNOS immunoreactivities in nasal mucosa, and increased levels of histamine, TXB2 and p-LTs in NLF were also observed. This model was not only capable of showing satisfactory symptomatic and pathophysiological changes in allergic rhinitis but also showed good responses to antihistamine treatment. The model can be established in six weeks. For the first time, respiratory rate was employed as an index to reflect the nasal blockage of guinea pigs and it proved to be a reliable indicator.
Analysis of Dicarboxylic Acids by Tandem Mass Spectrometry. High-Throughput Quantitative Measurement of Methylmalonic Acid in Serum, Plasma, and Urine (2001)

The method is specific for dicarboxylic acids. The LC-MS/MS analysis for MMA requires minimal chromatographic separation and takes <60 s per sample. The entire analysis, including sample preparation, for a batch of 100 specimens can be performed in <4 h.
Robust voice activity detection using cepstral features (2002)

This paper reviews algorithms which rely on the analysis of time domain samples to provide energy and zero-crossing rates, together with more recent algorithms that use different methods for speech detection. We then examine a different approach using cepstral analysis, showing a high degree of amplitude and noise level independence. We show that a cepstral based algorithm exhibits a high degree of independence to levels of background noise and successful speech end-pointing can be achieved via thresholding cepstral distance measures. Through the use of a noise code-book we are able to provide a successful reference for Euclidean distance measures in the voice detection algorithm.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Heparan Sulfate Degradation: Relation to Tumor Invasive and Metastatic Properties of Mouse B16 Melanoma Sublines (1983)

After transport in the blood and implantation in the microcirculation, metastatic tumor cells must invade the vascular endothelium and underlying basal lamina. Mouse B16 melanoma sublines were used to determine the relation between metastatic properties and the ability of the sublines to degrade enzymatically the sulfated glycosaminoglycans present in the extracellular matrix of cultured vascular endothelial cells. Highly invasive and metastatic B16 sublines degraded matrix glycosaminoglycans faster than did sublines of lower metastatic potential. The main products of this matrix degradation were heparan sulfate fragments. Intact B16 cells (or their cell-free homogenates) with a high potential for lung colonization degraded purified heparan sulfate from bovine lung at higher rates than did B16 cells with a poor potential for lung colonization. Analysis of the degradation fragments indicated that B16 cells have a heparan sulfate endoglycosidase. Thus the abilities of B16 melanoma cells to extravasate and successfully colonize the lung may be related to their capacities to degrade heparan sulfate in the walls of pulmonary blood vessels.
Columnar Alteration With Prominent Apical Snouts and Secretions (1998)

We have noted in breast biopsies performed for microcalcifications a spectrum of lesions in the terminal duct lobular unit (TDLU) characterized by columnar epithelial cells with prominent apical cytoplasmic snouts, intraluminal secretions, and varying degrees of nuclear atypia and architectural complexity. The appearance of some of these lesions is worrisome, but diagnostic difficulties arise because the histologic features do not fulfill established criteria for the diagnosis of atypical ductal hyperplasia or ductal carcinoma in situ (DCIS). We have termed such lesions columnar alteration with prominent apical snouts and secretions (CAPSS). The purpose of this study was to define the pathologic spectrum and mammographic features of these lesions. We reviewed histologic sections and mammograms from 100 consecutive breast biopsies performed for microcalcifications. The prevalence and histologic features of CAPSS and the association with other histologic findings were recorded. CAPSS was identified in 42% of cases. At the lower end of the spectrum were lesions similar to columnar alteration of lobules but in which apical cytoplasmic secretion and nuclear stratification were more pronounced and cells with a hobnail configuration were common. More advanced lesions showed columnar epithelial cell tufts, bridges, and micropapillations with prominent apical cytoplasmic snouts and with greater degrees of nuclear stratification and atypia. At the upper end of the spectrum were lesions that could arguably be considered DCIS. Calcifications were present within CAPSS in 74% of cases, were frequently psammomatous, and were typically nonbranching and often round on mammography. Columnar alteration of lobules was more common in biopsies with than without CAPSS (74 versus 36%, p < 0.001). Ductal carcinoma in situ was seen with similar frequency in biopsies with and without CAPSS (38 versus 41%). However, DCIS in cases with CAPSS was more often of the low-grade micropapillary-cribriform type than in cases without CAPSS (56 versus 17%, p < 0.01), and CAPSS and DCIS commonly coexisted in the same or adjacent TDLUs. In conclusion, 1) CAPSS encompasses a spectrum of lesions bounded at the lower end by columnar alteration of lobules and at the upper end by low-grade DCIS. Lesions recently described by Page as "hypersecretory hyperplasia with atypia" fall within this spectrum. 2) Some CAPSS lesions present architectural or cytologic features that create diagnostic difficulties and raise the possibility of atypical ductal hyperplasia or DCIS; however, the level of cancer risk associated with CAPSS lesions that do not fulfill established criteria for atypical ductal hyperplasia or DCIS is unknown and requires evaluation in follow-up studies.
Antifertility agents. 8. Seco steroids. 2. 5,6-Secoestradiol and some related compounds (1975)

Three ring beta-secoestradiols, 2alpha,3beta- and 2beta,3beta-2-ethyl-3-(p-hydroxyphenyl)-6beta-methyl-trans-bicyclo[4.3.0]nonan-7beta-ols, have been synthesized and some of them shown to possess significant antiimplantation activity in rats.
Large Area Fine Line Patterning by Scanning Projection Lithography (2005)

A new type of photolithography tool has been developed, addressing the specific needs of MCM manufacture. It is based on scanning projection exposure. It can expose panels at variable sizes up to 500 mm by 600 mm (typical laminate size), with an optical resolution of less than 5 gm and an overlay accuracy of 2 /spl mu/m (typical thin film design rules). With the exposure being a mask projection, mask damage and subsequent yield problems are generally avoided.
Involvement of Nav 1.8 sodium ion channels in the transduction of mechanical pain in a rodent model of osteoarthritis (2012)

These studies show for the first time that the Nav1.8 sodium channel is part of the molecular machinery involved in mechanotransduction of joint pain. Targeting the Nav1.8 sodium channel on joint nociceptors could therefore be useful for the treatment of OA pain, avoiding the unwanted side effects of non-selective nerve blocks.
General synthesis of N-substituted 2-azaadamantanes and their 4,8-disubstituted derivatives (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTGeneral synthesis of N-substituted 2-azaadamantanes and their 4,8-disubstituted derivativesJames G. Henkel, William C. Faith, and Jeffrey T. HaneCite this: J. Org. Chem. 1981, 46, 17, 3483–3486Publication Date (Print):August 1, 1981Publication History Published online1 May 2002Published inissue 1 August 1981https://pubs.acs.org/doi/10.1021/jo00330a020https://doi.org/10.1021/jo00330a020research-articleACS PublicationsRequest reuse permissionsArticle Views308Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts