gebhart/patrepeval huggingface dataset:
Rapid functionalization of cantilever array sensors by inkjet printing (2004)

The controlled deposition of functional layers is the key to converting nanomechanical cantilevers into chemical or biochemical sensors. Here, we introduce inkjet printing as a rapid and general method to coat cantilever arrays efficiently with various sensor layers. Self-assembled monolayers of alkanethiols were deposited on selected Au-coated cantilevers and rendered them sensitive to ion concentrations or pH in liquids. The detection of gene fragments was achieved with cantilever sensors coated with thiol-linked single-stranded DNA oligomers on Au. A selective etch protocol proved the uniformity of the monolayer coatings at a microscopic level. A chemical gas sensor was fabricated by printing thin layers of different polymers from dilute solutions onto cantilevers. The inkjet method is easy to use, faster and more versatile than coating via microcapillaries or the use of pipettes. In addition, it is scalable to large arrays and can coat arbitrary structures in non-contact.
Cardiac failure in children (2008)

In children, cardiac failure is most often caused congenital disease andcardiomyopathy. These causes are significantly those responsible forthe condition in adults, which include coronary artery disease and hypertension.
Characteristics of electron cyclotron resonance plasma sources (1989)

Electron cyclotron resonance plasmas were excited at a frequency of 2.45 GHz in chambers with diameters smaller and larger than cutoff ones for fundamental TE and TM modes. Microwaves were introduced in the plasma chambers from the cw generator via a vacuum window and the microwave transmission line. It was found that microwave breakdowns at pressures 10−5–10−1 Torr (argon, nitrogen, oxygen) occurred at sites where the static magnetic field was 875 G (or close). In chambers with diameter smaller than cutoff, the breakdown occurred only near the vacuum window. A high absorption level of microwave power (&amp;gt;95%) was achieved in all explored chambers at microwave power P=300–1000 W. It occurred at areas where the static magnetic field was ∼875 G and the plasma density was Ncr=7.4×1010 cm−3. A high microwave power absorption level resulted in high plasma density up to Ne=1012 cm−3 at P=1000 W. No saturation in Ne=f(P) was observed. The shape and location of the high plasma density area was determined by microwave matching tuning, magnetic field and its configuration, and source chamber size.
Spin waves excited by dc currents injected into single ferromagnetic thin films (2007)

It is well known theoretically and experimentally that spin waves can be excited by current in magnetic trilayer and multilayer structures where the current is spin polarized by one of the magnetizations and interacts with the others. However, it has also been observed experimentally that a nonpolarized dc current flowing through a point contact into a single ferromagnetic thin film can induce spin waves. We investigate this phenomenon using a model in which the spins of the conduction electrons interact with a nonuniform magnetization and the current is treated perturbatively. To a first-order approximation, the spin-wave modes excited by the injected current have been characterized and the relationship between the dynamic properties of the spin waves and the applied current and applied magnetic field has been established. Calculated results are then used for comparison with the experimental observations.
The discovery and application of gene fusions in prostate cancer (2008)

Chromosomal rearrangements play a causal role in haematological and mesenchymal malignancies. Importantly, the resulting gene fusions can serve as specific therapeutic targets, as exemplified by the development of imatinib (Gleevec), which specifically inhibits the BCR-ABL gene fusion product that defines chronic myeloid leukaemia. Recently, gene fusions involving the prostate-specific gene transmembrane protease, serine 2 (TMPRSS2) and members of the erythroblastosis virus E26 transforming sequence (ETS) family of transcription factors were identified in most of PSA-screened prostate cancers. In this review, we summarize the identification, characterization and detection of TMPRSS2:ETS gene fusions and their role in prostate cancer development. We also discuss the discovery of additional 5' partners that define distinct classes of ETS gene fusions based on the prostate specificity and androgen responsiveness of the 5' partner. Additionally, we also summarize conflicting reports about associations between gene fusion status and patient outcome. The specificity of ETS gene fusions in prostate cancer suggests that they may have causal roles in prostate cancer and suggest utility in prostate cancer detection, stratification and treatment.
Multidrug resistance in enteric and other Gram-negative bacteria (1996)

In Gram-negative bacteria, multidrug resistance is a term that is used to describe mechanisms of resistance by chromosomal genes that are activated by induction or mutation caused by the stress of exposure to antibiotics in natural and clinical environments. Unlike plasmid-borne resistance genes, there is no alteration or degradation of drugs or need for genetic transfer. Exposure to a single drug leads to cross-resistance to many other structurally and functionally unrelated drugs. The only mechanism identified for multidrug resistance in bacteria is drug efflux by membrane transporters, even though many of these transporters remain to be identified. The enteric bacteria exhibit mostly complex multidrug resistance systems which are often regulated by operons or regulons. The purpose of this review is to survey molecular mechanisms of multidrug resistance in enteric and other Gram-negative bacteria, and to speculate on the origins and natural physiological functions of the genes involved.
Abstract 1186: PTEN expression predicts biochemical recurrence in prostate cancer (2010)

Abstract Prostate cancer is the most commonly diagnosed malignancy in men, but many patients have indolent disease that is unlikely to cause significant morbidity or mortality. Biomarkers that accurately predict disease severity would enable physicians to more appropriately manage patient care. Data from previous studies indicated that the molecular status of PTEN (phosphatase and tensin homolog) might be prognostic in prostate cancer patients after radical prostatectomy (RP). PTEN expression was evaluated on surgical specimens by IHC using anti-PTEN antibody 138G6 (Cell Signaling Technology) in 132 men treated with RP. Approximately half the patients also had neoadjuvant hormonal blockade therapy (BAT). PTEN IHC scores were derived from the weighted average of percent positive cells x intensity (0, 1 or 2). PTEN status (positive or negative) was determined using a predefined IHC score of ≤ 20 for negatives. Using this scoring system, 24% of the tumors were PTEN negative. Association with biochemical recurrence was evaluated using Cox PH models and likelihood ratio tests. Since we were testing a prior hypothesis about the prognostic utility of PTEN, the corresponding p-values are one-sided. In a univariate analysis on the overall series, PTEN status was associated with biochemical recurrence (p-value = 0.0046, HR = 2.3; 95% CI: 1.27, 4.33). Most of the clinical parameters typically associated with recurrence were affected by neoadjuvant therapy and rendered uninterpretable. Nevertheless, pathological tumor stage (p-value = 4.3 × 10−6, HR = 6.0; 95% CI: 2.35, 15.22) was still associated with outcome. In multivariate analyses, PTEN status was a significant predictor of outcome after adjusting for pathological stage (p-value = 0.009), neoadjuvant therapy (p-value = 0.014), or both (p-value = 0.04). These data support the hypothesis that PTEN status predicts outcome after RP, and suggest further, that PTEN may be a clinically important molecular marker for defining aggressive prostate cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1186.
Bonded silicon-on-sapphire wafers and devices (1992)

Silicon-on-sapphire (SOS) has been prepared by direct wafer bonding. The silicon layer was thinned to about 10 μm by mechanical grinding and chemical etching. P-N junction diodes were fabricated in the bonded SOS and compared with epitaxially grown SOS. The reverse bias leakage current was almost 15× less in the bonded SOS. A generation lifetime of 10 μs can be estimated from the junction leakage. The effects of processing temperatures on the bonded SOS were also studied.
Stereo coding for the ITU-T G.719 codec (2011)

This thesis presents a stereo coding architecture for the ITU-T G.719 fullband mono codec. G.719 is suitable for teleconferencing applications with a competitive audio quality for speech and audio signals that are encoded at 32, 48 and 64 kbps. The proposed stereo architecture comprises parametric stereo coding where the spatial properties of the stereo channels are modeled with the use of parameters, which are encoded and transmitted to the decoder together with an encoded downmix of the stereo channels. The stereo architecture has been implemented in MATLAB with an external mono coding using a floating point ANSI-C implementation of the ITU-T G.719 codec. Two parametric stereo models have been implemented in a framework operating in the complex-valued Modified Discrete Fourier Transform (MDFT) domain. The first model is based on the inter-channel cues that represent level differences, time differences and coherences between the stereo channels. The cues approximate the corresponding interaural cues that characterize our localization of sound in space. The second model is based on the Karhunen-Loève Transform (KLT) with the associated rotation angles, the inter-channel time differences and the residual scaling parameters. An improved MDFT domain extraction of the inter-channel time difference between the stereo channels has been used for both stereo models. The extracted stereo parameters have been non-uniformly quantized based on the spatial accuracy and the frequency dependency of the human auditory system. The data rate of the stereo parameters has been estimated for each model to around 4 kbps. As a result G.719 has been used as a core codec at 44 and 60 kbps in order to subjectively evaluate the performance of the fullband stereo codec at 48 and 64 kbps. In the comparison with G.719 dual mono coding, i.e. independent mono coding of the stereo channels, the evaluation showed a higher performance of the proposed stereo models for complex clean and reverberant speech signals. However, no consistent gain of the parametric stereo coding was revealed for noisy speech, mixed content and music signals. In addition, the first stereo model showed consistently a slightly higher performance than the second model in the subjective evaluation but with no significant difference. The results revealed a high potential for parametric stereo coding using the ITU-T G.719 codec. In comparison to the existing stereo codecs 3GPP AMR-WB+ and 3GPP eAAC+ the average performance was better at the equal bitrate of 48 kbps.
Lymphocyte function—associated antigen 1 overexpression and t cell autoreactivity (1994)

LFA-1 overexpression may contribute to T cell autoreactivity.
Fast and flexible application-level networking on exokernel systems (2002)

Application-level networking is a promising software organization for improving performance and functionality for important network services. The Xok/ExOS exokernel system includes application-level support for standard network services, while at the same time allowing application writers to specialize networking services. This paper describes how Xok/ExOS's kernel mechanisms and library operating system organization achieve this flexibility, and retrospectively shares our experiences and lessons learned (both positive and negative). It also describes how we used this flexibility to build and specialize three network data services: the Cheetah HTTP server, the webswamp Web benchmarking tool, and an application-level TCP forwarder. Overall measurements show large performance improvements relative to similar services built on conventional interfaces, in each case reaching the maximum possible end-to-end performance for the experimental platform. For example, Cheetah provides factor of 2--4 increases in throughput compared to highly tuned socket-based implementations and factor of 3--8 increases compared to conventional systems. Webswamp can offer loads that are two to eight times heavier. The TCP forwarder provides 50--300% higher throughput while also providing end-to-end TCP semantics that cannot be achieved with POSIX sockets. With more detailed measurements and profiling, these overall performance improvements are also broken down and attributed to the specific specializations described, providing server writers with insights into where to focus their optimization efforts.
The LEAF picosecond pulse radiolysis facility at Brookhaven National Laboratory (2004)

The BNL Laser-Electron Accelerator Facility (LEAF) uses a laser-pulsed photocathode, radio-frequency electron gun to generate ⩾7 ps pulses of 8.7 MeV electrons for pulse radiolysis experiments. The compact and operationally simple accelerator system includes synchronized laser pulses that can be used to probe or excite the electron-pulsed samples to examine the dynamics and reactivity of chemical species on the picosecond time scale.
Synthetic Studies of Cortistatin A Analogue from the CD-Ring Fragment of Vitamin D<sub>2</sub> (2013)

Syntheses of analogue compounds of cortistatin A (1), an anti-angiogenic steroidal alkaloid from Indonesian marine sponge, were investigated by utilizing the CD-ring fragment of vitamin D₂. The incidental preparation of a new analogue having CD-cis-fused skeleton and its biological evaluation revealed the importance of the CD-trans-fused structure for the potent and selective antiproliferative activity of 1 against human umbilical vein endothelial cells (HUVECs).
Quadrature chaos-shift keying: theory and performance analysis (2001)

In this brief, we propose a multilevel version of the differential chaos shift keying (DCSK) telecommunication system. The scheme, that we call quadrature chaos shift keying (QCSK), is based upon the generation of an orthogonal basis of chaotic functions. QCSK is characterized by an increased data rate with respect to DCSK, with the same bandwidth occupation. The price for the performance enhancement is the increased complexity of both the transmitter and the receiver.
Direct Activation of Mitogen-Activated Protein Kinase Kinase Kinase MEKK1 by the Ste20p Homologue GCK and the Adapter Protein TRAF2 (2002)

Mitogen-activated protein kinase (MAPK) pathways coordinate critical cellular responses to mitogens, stresses, and developmental cues. The coupling of MAPK kinase kinase (MAP3K) --> MAPK kinase (MEK) --> MAPK core pathways to cell surface receptors remains poorly understood. Recombinant forms of MAP3K MEK kinase 1 (MEKK1) interact in vivo and in vitro with the STE20 protein homologue germinal center kinase (GCK), and both GCK and MEKK1 associate in vivo with the adapter protein tumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2). These interactions may couple TNF receptors to the SAPK/JNK family of MAPKs; however, a molecular mechanism by which these proteins might collaborate to recruit the SAPKs/JNKs has remained elusive. Here we show that endogenous GCK and MEKK1 associate in vivo. In addition, we have developed an in vitro assay system with which we demonstrate that purified, active GCK and TRAF2 activate MEKK1. The RING domain of TRAF2 is necessary for optimal in vitro activation of MEKK1, but the kinase domain of GCK is not. Autophosphorylation within the MEKK1 kinase domain activation loop is required for activation. Forced oligomerization also activates MEKK1, and GCK elicits enhanced oligomerization of coexpressed MEKK1 in vivo. These results represent the first activation of MEKK1 in vitro using purified proteins and suggest a mechanism for MEKK1 activation involving induced oligomerization and consequent autophosphorylation mediated by upstream proteins.
Translational Properties of mHNA, a Messenger RNA Containing Anhydrohexitol Nucleotides (2001)

Short messenger RNAs (mRNAs) with hexitol residues in two codons were constructed and their properties were studied in an Escherichia coli in vitro translation system. The replacement of the natural ribonucleotides of mRNA in the AUG start codon and the UUC second codon by hexitol nucleotides did not influence the main steps of translation, as indicated by the same level of binding of mRNA with or without hexitol residues under P-site conditions, and the same yield of tRNA binding to the P- and A-sites. Moreover, both peptide formation and translocation took place on mRNAs with hexitol residues. The presence of an A-type messenger hexitol nucleic acid (mHNA)-transfer RNA (tRNA) duplex is important for efficient translation and the 2'-OH function in mRNA is not necessary for binding and movement through the ribosome. Groove shape recognition of the codon-anticodon complex, more than hydrogen-bond interactions of ribose residues in mRNA, is an important factor for correct translation.
Prevalence of mild cognitive impairment is higher in men (2010)

Our study suggests that approximately 16% of elderly subjects free of dementia are affected by MCI, and amnestic MCI is the most common type. The higher prevalence of MCI in men may suggest that women transition from normal cognition directly to dementia at a later age but more abruptly.
Vitaminy rozpustné v tucích (2008)

Clanek shrnuje soucasne poznatky o vitaminech rozpustných v tucich. Uvadi jejich zdroje, význam pro lidský organizmus, přiciny a projevy jejich nedostatku i připadneho předavkovani, k němuž (na rozdil od vitaminů rozpustných ve vodě) může dojit.
Cyclophosphamide stimulates lung fibroblasts to release neutrophil and monocyte chemoattractants (2001)

Cyclophosphamide is an alkylating antineoplastic agent used in several conditions. However, little is known about the mechanism of its pulmonary toxicity. In the present study, we determined that human lung fibroblasts release activity for neutrophils and monocytes in response to cyclophosphamide in a dose- and time-dependent manner. Checkerboard analysis revealed that both neutrophil and monocyte activities were chemotactic. The release of chemotactic activity was inhibited by lipoxygenase inhibitors and cycloheximide. Molecular-sieve column chromatography revealed that both neutrophil (NCA) and monocyte (MCA) chemotactic activities had multiple peaks. NCA was inhibited by a leukotriene B(4) receptor antagonist and anti-interleukin-8 and anti-granulocyte colony-stimulating factor antibodies. MCA was attenuated by a leukotriene B(4) receptor antagonist and anti-monocyte chemoattractant protein-1 and anti-granulocyte-macrophage colony-stimulating factor antibodies. The concentrations of interleukin-8, granulocyte colony-stimulating factor, monocyte chemoattractant protein-1, and granulocyte-macrophage colony-stimulating factor significantly increased in response to cyclophosphamide. These data suggest that lung fibroblasts may modulate inflammatory cell recruitment into the lung by releasing NCA and MCA in response to cyclophosphamide.
Electromagnetic Lens-focusing Antenna Enabled Massive MIMO (2013)

Massive multiple-input multiple-output (MIMO) techniques have been recently advanced to tremendously improve the performance of wireless networks. However, the use of very large antenna arrays brings new issues, such as the significantly increased hardware cost and signal processing cost and complexity. In order to reap the enormous gain of massive MIMO and yet reduce its cost to an affordable level, this paper proposes a novel system design by integrating an electromagnetic (EM) lens with the large antenna array, termed \emph{electromagnetic lens antenna} (ELA). An ELA has the capability of focusing the power of any incident plane wave passing through the EM lens to a small subset of the antenna array, while the location of focal area is dependent on the angle of arrival (AoA) of the wave. As compared to conventional antenna arrays without the EM lens, the proposed system can substantially reduce the number of required radio frequency (RF) chains at the receiver and hence, the implementation costs. In this paper, we investigate the proposed system under a simplified single-user uplink transmission setup, by characterizing the power distribution of the ELA as well as the resulting channel model. Furthermore, by assuming antenna selection used at the receiver, we show the throughput gains of the proposed system over conventional antenna arrays given the same number of selected antennas.
Mycobacterium bovis Bacillus Calmette-Guérin Vaccination Mobilizes Innate Myeloid-Derived Suppressor Cells Restraining In Vivo T Cell Priming via IL-1R–Dependent Nitric Oxide Production (2010)

Early immune response to the largely used Mycobacterium bovis bacillus Calmette-Guérin (BCG) intradermal vaccine remains ill defined. Three days after BCG inoculation into the mouse ear, in addition to neutrophils infiltrating skin, we observed CD11b(+)Ly-6C(int)Ly-6G(-) myeloid cells. Neutrophil depletion markedly enhanced their recruitment. These cells differed from inflammatory monocytes and required MyD88-dependent BCG-specific signals to invade skin, whereas neutrophil influx was MyD88 independent. Upon BCG phagocytosis, CD11b(+)Ly-6C(int)Ly-6G(-) cells produced NO, which required the IL-1 receptor. Despite NO production, they were unable to kill BCG or the nonpathogenic Mycobacterium smegmatis. However, they markedly impaired T cell priming in the draining lymph node. Their elimination by all-trans retinoid acid treatment increased the number of IFN-gamma-producing CD4 T cells. Thus, BCG vaccination recruits innate myeloid-derived suppressor cells, akin to mouse tumor-infiltrating cells. These propathogenic cells dampen the early T cell response and might facilitate BCG persistence.
Dexamethasone differentially affects the levels of calcitonin and calcitonin gene-related peptide mRNAs expressed in a human medullary thyroid carcinoma cell line. (1986)

The TT cells are a continuous line of human C-cells derived from a medullary thyroid carcinoma. These cells produce large quantities of calcitonin and calcitonin gene-related peptide (CGRP) by the differential splicing of a single calcitonin gene transcript. We have used specific cDNA probes for calcitonin and CGRP to study the regulation of the calcitonin gene by dexamethasone, a synthetic glucocorticoid. Northern blot analysis of total cellular RNA isolated from the TT cells showed hybridization of the calcitonin probe to 3600- and 1000-base RNA species. The CGRP probe hybridized to 3600- and 1050-base RNA species. Dexamethasone treatment (10(-9) to 10(-5) M) of TT cells (for 6 days) caused a dosage-dependent increase in calcitonin mRNA levels and a decrease in CGRP mRNA levels. These findings were confirmed in time course studies where dexamethasone treatment (10(-6) M) caused a 2-13-fold increase in calcitonin mRNA and a 40-60% decrease in CGRP mRNA between 4 and 6 days of treatment; the effect was reversible after dexamethasone withdrawal. After excluding an effect of dexamethasone on calcitonin and CGRP mRNA stability, we have concluded that dexamethasone affects the splicing mechanism to favor production of calcitonin mRNA over CGRP mRNA.