gebhart/patrepeval huggingface dataset:
Effect of RNase III on efficiency of translation of bacteriophage T7 lysozyme mRNA (1978)

RNase III had no positive effect on the translation of bacteriophage T7 lysozyme mRNA in vivo or in vitro. The time of appearance and quanity of lysozyme in T7-infected E. coli BL107, an RNase III- strain, and T7-infected E. coli BL15, a nearly isogenic RNase III+ strain, were indistinguishable. Nearly identical patterns of lysozyme mRNA activity were obtained when RNA extracted at different times after infection of RNase III+ and RNase III- hosts was translated in cell-free extracts of E. coli containing or lacking RNase III. Exposure of RNA extracted from T7-infected E. coli BL107 (RNase III-) to purified RNase III did not increase the lysozyme mRNA activity of this RNA. The only result that implied that RNase III has a differential effect on the translatability of the lysozyme mRNA was the translation of fractionaed RNA from T7-infected E. coli BL107. Translation of the smallest and largest lysozyme messages, 0.33 x 10(6) and 4 x 10(6) to 5 x 10(6) daltons, was the most inefficient in RNase III- cell-free extracts as compared to RNase III+ cell-free translation. The translation of the most abundant, medium-sized lysozyme mRNA between 0.9 x 10(6) and 1.5 x 10(6) daltons was the least affected by the absence of RNase III. The existence of a lag between the appearance of lysozyme mRNA and the appearance of lysozyme in T7 infection was confirmed. In these studies a very rapid method of RNA extraction was used, eliminating the possibility of continued RNA transcription during cell collection and RNA extraction. With this method of analysis, the length of the lag period was established at about 3 min. The possibility that RNase III is the controlling element of the lag period was eliminated by these investigations.
Total Synthesis of (±)-Marinopyrrole A via Copper-Mediated N-Arylation (2010)

The total synthesis of the racemic form of the marine alkaloid marinopyrrole A is described. The characteristic 1,3'-bipyrrole core was constructed by a copper-mediated N-arylation process under microwave irradiation.
Surface-Modified and Internally Cationic Polyamidoamine Dendrimers for Efficient siRNA Delivery (2008)

A novel internally quaternized and surface-acetylated poly(amidoamine) generation four dendrimer (QPAMAM-NHAc) was synthesized and evaluated for intracellular delivery of siRNA. The proposed dendrimer as a nanocarrier possesses the following advantages: (1) modified neutral surface of the dendrimer for low cytotoxicity and enhanced cellular internalization; (2) existence of cationic charges inside the dendrimer (not on the outer surface) resulting in highly organized compact nanoparticles, which can potentially protect nucleic acids from degradation. The properties of this dendrimer were compared with PAMAM-NH 2 dendrimer, possessing surface charges, and with an internally quaternized charged and hydroxyl-terminated QPAMAM-OH dendrimer. Atomic force microscopy studies revealed that internally charged and surface neutral dendrimers, QPAMAM-OH and QPAMAM-NHAc, formed well-condensed, spherical particles (polyplexes) with siRNA, while PAMAM-NH 2 resulted in the formation of nanofibers. The modification of surface amine groups to amide significantly reduced cytotoxicity of dendrimers with QPAMAM-NHAc dendrimer showing the lowest toxicity. Confocal microscopy demonstrated enhanced cellular uptake and homogeneous intracellular distribution of siRNA delivered by the proposed QPAMAM-NHAc nanocarrier. The results clearly demonstrated distinct advantages of developed QPAMAM-NHAc/siRNA polyplexes over the existing nucleic acid dendrimeric carriers.
Isoforms of glucose transporter in the iris-ciliary body. (1995)

Isoforms of the facilitated glucose transporter (GLUT) were identified in the iris-ciliary body. Western blot analysis showed that GLUT1 and GLUT4 proteins were expressed in the rat iris-ciliary body. In addition, the content of GLUT proteins in the rabbit iris-ciliary body was estimated by specific [3H]cytochalasin B binding assay. The content of GLUT proteins was found to be more abundant in the iris-ciliary body than in the cerebral cortex. The utilization of glucose in the iris-ciliary body and the transport of glucose into the aqueous humor are suggested to be mediated by GLUT1 and GLUT4 proteins.
Fucosylated Antigens in Cancer: An Alliance toward Tumor Progression, Metastasis, and Resistance to Chemotherapy (2018)

Aberrant glycosylation of tumor cells is recognized as a universal hallmark of cancer pathogenesis. Overexpression of fucosylated epitopes, such as type I (H1, Lewis<sup>a</sup>, Lewis<sup>b</sup>, and sialyl Lewis<sup>a</sup>) and type II (H2, Lewis<sup>x</sup>, Lewis<sup>y</sup>, and sialyl Lewis<sup>x</sup>) Lewis antigens, frequently occurs on the cancer cell surface and is mainly attributed to upregulated expression of pertinent fucosyltransferases (FUTs). Nevertheless, the impact of fucose-containing moieties on tumor cell biology is not fully elucidated yet. Here, we review the relevance of tumor-overexpressed FUTs and their respective synthesized Lewis determinants in critical aspects associated with cancer progression, such as increased cell survival and proliferation, tissue invasion and metastasis, epithelial [corrected] to mesenchymal transition, endothelial and immune cell interaction, angiogenesis, multidrug resistance, and cancer stemness. Furthermore, we discuss the potential use of enhanced levels of fucosylation as glycan biomarkers for early prognosis, diagnosis, and disease monitoring in cancer patients.
Outbreak of Aseptic Meningitis associated with Mass Vaccination with a Urabe-containing Measles-Mumps-Rubella Vaccine: Implications for Immunization Programs (2000)

A mass immunization campaign with a Urabe-containing measles-mumps-rubella vaccine was carried out in 1997 in the city of Salvador, northeastern Brazil, with a target population of children aged 1-11 years. There was an outbreak of aseptic meningitis following the mass campaign. Cases of aseptic meningitis were ascertained through data collected from the records of children admitted to the local referral hospital for infectious diseases between March and October of 1997, using previously defined eligibility criteria. Vaccination histories were obtained through home visits or telephone calls. Eighty-seven cases fulfilled the study criteria. Of those, 58 cases were diagnosed after the vaccination campaign. An elevated risk of aseptic meningitis was observed 3 weeks after Brazil's national vaccination day compared with the risk in the prevaccination period (relative risk = 14.3; 95% confidence interval: 7.9, 25.7). This result was confirmed by a case series analysis (relative risk = 30.4; 95% confidence interval: 11.5, 80.8). The estimated risk of aseptic meningitis was 1 in 14,000 doses. This study confirms a link between measles-mumps-rubella vaccination and aseptic meningitis. The authors discuss the implications of this for the organization and planning of mass immunization campaigns.
Measurement of Uveoscleral Outflow in Humans (2001)

Mayo Clinic, Rochester, Minnesota Address correspondence and reprint requests to Dr. Richard F. Brubaker, Professor of Ophthalmology, Emeritus, Mayo Clinic 601 Memorial Parkway SW, Rochester, MN 55902.
Comprehensive Sequence Analysis of 24,783 Barley Full-Length cDNAs Derived from 12 Clone Libraries (2011)

Full-length cDNA (FLcDNA) libraries consisting of 172,000 clones were constructed from a two-row malting barley cultivar (Hordeum vulgare 'Haruna Nijo') under normal and stressed conditions. After sequencing the clones from both ends and clustering the sequences, a total of 24,783 complete sequences were produced. By removing duplicates between these and publicly available sequences, 22,651 representative sequences were obtained: 17,773 were novel barley FLcDNAs, and 1,699 were barley specific. Highly conserved genes were found in the barley FLcDNA sequences for 721 of 881 rice (Oryza sativa) trait genes with 50% or greater identity. These FLcDNA resources from our Haruna Nijo cDNA libraries and the full-length sequences of representative clones will improve our understanding of the biological functions of genes in barley, which is the cereal crop with the fourth highest production in the world, and will provide a powerful tool for annotating the barley genome sequences that will become available in the near future.
Mechanisms and implications of imatinib resistance mutations in BCR-ABL (2003)

The most common mechanism of relapse for CML patients treated with Imatinib is the appearance of point mutations in the BCR-ABL oncogene that confer resistance to this drug. Insights into the emerging problem of resistance should promote the rational development of alternative, synergistic, and potentially curative treatment strategies.
Control of HVDC Light System Using Conventional and Direct Current Vector Control Approaches (2010)

With the advance of power electronic technology, the application of HVdc light systems based on voltage-source insulated gate bipolar transistor (IGBT) converters has increased rapidly in renewable, microgrid, and electric power systems. This paper proposes an optimal control strategy for an HVdc light system using a direct current vector control mechanism. The proposed approach is compared with the traditional vector control method for different HVdc control requirements, such as active power, reactive power, and grid voltage support control. A limitation of the conventional control mechanism is analyzed through a theoretical study and computer simulation. Closed-loop control evaluation demonstrates that the proposed approach works well for HVdc light system control both within and beyond the physical constraints of the system, such as rated power and saturation of pulse width modulation (PWM). The evaluation also shows that the conventional control technique could result in over voltage and system oscillation, especially when the controller operates beyond the PWM saturation limit.
Cure Cycle Optimization for the Reduction of Processing-Induced Residual Stresses in Composite Materials (1993)

The control and reduction of processing-induced residual stresses has been investigated by modifying processing conditions for a graphite/BMI composite material. The effects of dwell temperature, dwell time, cool-down rate, cool-down pres sure, and postcure on residual stresses were investigated using unsymmetric cross-ply laminates. The effects on transverse mechanical properties were also measured. Experi mental results have shown that residual stresses can be reduced by as much as 25-30% while retaining or enhancing transverse mechanical properties by curing at lower tempera tures for longer times or utilizing an intermediate low-temperature dwell in three-step cure cycles. Overall process cycle times are not lengthened for three-step curing.
2-D WDM optical interconnections using multiple-wavelength VCSEL's for simultaneous and reconfigurable communication among many planes (1993)

A novel interconnection configuration in which one two-dimensional plane can communicate simultaneously and reconfigurably with many planes by using wavelength-division-multiplexing (WDM) is proposed and analyzed. This system incorporates arrays of multiple-wavelength vertical-cavity surface-emitting lasers (VCSELs) as well as wavelength-selective detecting planes. High signal contrast ratio with low power penalty can be achieved for a channel separation>30 nm, limited only by the detector spectral response. By using WDM pixels, the system capacity is significantly enhanced as compared to more traditional techniques.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Zeolites and Clay Minerals As Sorbents and Molecular Sieves (1978)

To mineralogists and earth scientists the zeolites and clay minerals have been of great interest for a very long time. Chemists and engineers became interested primarily in the years following the Second War, and their attention has for the most part been concentrated upon the zeolits. So great has been the effort expended that the volume of research on these minerals is now too large to digest and summarize in a single, reasonably critical volume. As with other technical and scientific areas of rapid growth a point has been reached where monographs are required, each of which reviews a chosen part of the broad field. It was on this basis that the present book was planned. A number of monographs, either with a single author, or (more commonly) cooperative efforts by a number of authors, have appeared which deal with clay minerals or with zeolites. Nevertheless the coverage in certain areas is inadequate, and the present book will, it is hoped, fill one of these gaps. It provides an account of sorption and intercalation by zeolites and clay minerals, emphasizing the structural, physico-chemical and theoretical bases of the phenomena observed. In some respects at least, clay mineral and zeolites provide model sorbents, which also have a growing range of applications both in laboratory practice and in industry.
Two-dimensional array transducers using thick film connection technology (1993)

A connection technique for two-dimensional array ultrasound transducers developed by combining a conductive lambda/4 mismatching layer with a multi-layer ceramic (MLC) connector using thick-film microelectronic technology is described. The connector consists of 20 thick films of alumina and screen printed metallization with customized interconnections between the layers called vias. Ten ground layers are interleaved between ten signal layers to reduce elecrical crosstalk. A lambda/4 mismatching layer of conductive epoxy is bonded between each PZT element and the silver metal pad of the MLC connector to provide an effective low impedance backing. In the current configuration, a 16x16 transducer array, 0.6 mm element spacing, is expanded to a 16x16 grid of connector pins at a standard spacing of 2.5 mm. Vector impedance, sensitivity, bandwidth, interelement uniformity, and crosstalk are all in good agreement with arrays of conventional fabrication. However, an array with MLC connector can be fabricated more quickly independent of the number of elements.
Dynamical behavior of optomechanical induced transparency in a silica microresonator (2014)

In an optomechanical resonator, the optical and mechanical excitations can be coherent converted, which induces a transparency window for a weak probe laser beam. Here, we report an experimental study of transient optomechanical-induced transparency (OMIT) using the silica microcavity with breathing modes. We have also reported experimental studies on both OMIT and light storage under nearly the same conditions also illustrates the connections between these two closely related processes. As straightforward extension of the light storage process is to use a readout pulse that couples to a different optical mode. The resulting readout process enables optical wavelength or optical mode conversion.
Dexamethasone regulates the beta-adrenergic receptor subtype expressed by 3T3 L1 preadipocytes and adipocytes. (1982)

The subtype of the beta-adrenergic receptor expressed in 3T3-L1 preadipocytes and adipocytes differentiated with dexamethasone and methylisobutylxanthine was determined by comparing the affinity of the receptors for epinephrine, norepinephrine, and beta-1 and beta-2 selective antagonist, 8-fold more avidly than adipocyte receptors. In contrast, adipocyte beta-receptors had a 10-fold higher affinity for epinephrine than for norepinephrine and complexed the beta-2 selective agonist zinterol with a 20-fold higher affinity than preadipocyte receptors. Hofstee plots and computer analyses of the binding data revealed that the populations of beta-1 receptors in preadipocytes and beta-2 receptors in adipocytes were nearly homogeneous. Preliminary characterizations of the beta-receptor phenotype in (nondifferentiating) 3T3-C2 cells treated with dexamethasone and methylisobutylxanthine and 3T3-422A adipocytes differentiated with insulin indicated that the expression of beta-2 receptors was not correlated with differentiation, but rather with exposure of the cells to dexamethasone and methylisobutylxanthine. The regulator of beta-receptor subtype was identified as the glucocorticoid analog, dexamethasone, by employing 3T3-L1 adipocytes which were stimulated to differentiate with methylisobutylxanthine and insulin. Detailed binding studies showed that under these conditions the adipocyte receptors retain beta-1 character. Subsequent treatment with 0.5 microM dexamethasone promoted the loss of beta-1 receptors, the appearance of beta-2 receptors, and a net 2- to 3-fold increase in the number of beta-receptors. Dexamethasone effected a complete switch from beta-1 to beta-2 subtype at concentrations as low as 2.5 nM while other steroids were ineffective below a concentration of 10 microM.
Malignant hypertension and hypertensive emergencies. (1998)

Hypertensive emergencies and urgencies are important causes of morbidity and mortality. Malignant hypertension is a hypertensive urgency characterized by grade III/IV retinopathy and widespread endothelial damage. Control of BP is essential in the treatment of these disorders. The effects of hypertension on target organ function need to be balanced against the risks of excessive BP lowering. In hypertensive emergencies, BP should be lowered within minutes with parenteral agents to prevent critical end-organ damage. In hypertensive urgencies, BP can be lowered more slowly over several hours, often with oral agents, to avoid a detrimental fall in BP. The absolute indications for treatment and the optimal therapy depend on the underlying condition.
Expanded polyglutamines in Caenorhabditis elegans cause axonal abnormalities and severe dysfunction of PLM mechanosensory neurons without cell death (2001)

Huntington's disease (HD) is a dominant neurodegenerative disease caused by polyglutamine (polyQ) expansion in the protein huntingtin (htt). HD pathogenesis appears to involve the production of mutated N-terminal htt, cytoplasmic and nuclear aggregation of htt, and abnormal activity of htt interactor proteins essential to neuronal survival. Before cell death, neuronal dysfunction may be an important step of HD pathogenesis. To explore polyQ-mediated neuronal toxicity, we expressed the first 57 amino acids of human htt containing normal [19 Gln residues (Glns)] and expanded (88 or 128 Glns) polyQ fused to fluorescent marker proteins in the six touch receptor neurons of Caenorhabditis elegans. Expanded polyQ produced touch insensitivity in young adults. Noticeably, only 28 +/- 6% of animals with 128 Glns were touch sensitive in the tail, as mediated by the PLM neurons. Similar perinuclear deposits and faint nuclear accumulation of fusion proteins with 19, 88, and 128 Glns were observed. In contrast, significant deposits and morphological abnormalities in PLM cell axons were observed with expanded polyQ (128 Glns) and partially correlated with touch insensitivity. PLM cell death was not detected in young or old adults. These animals indicate that significant neuronal dysfunction without cell death may be induced by expanded polyQ and may correlate with axonal insults, and not cell body aggregates. These animals also provide a suitable model to perform in vivo suppression of polyQ-mediated neuronal dysfunction.
Proton-Gated Channels in PC12 Cells (2002)

Acid-sensing ion channels (ASICs) are expressed in various sensory and central neurons. The functional role of these channels remains elusive. Complex subunit combinations and lack of specific blockers for native receptors are likely to contribute to the difficulty of resolving the function of ASICs. Finding a neuronal cell line, which expresses a single population of ASICs, should prove to be useful in delineating the function of individual ASICs. Using patch-clamp, Ca(2+)-imaging, and RT-PCR techniques, we have explored the existence of ASICs in PC12 cells, a clonal neuronal cell line. Fast drops of extracellular pH activated transient inward currents in PC12 cells with pH(0.5) at 6.0-6.2. The ASICs in PC12 cells were selective for Na(+) with significant Ca(2+) permeability. Currents in PC12 cells were blocked by the nonselective ASIC blocker amiloride. PcTX1, a specific homomeric ASIC1a blocker, also blocked the ASIC currents with an IC(50) of approximately 1.5 nM. RT-PCR demonstrated the existence of ASIC1a transcript in both undifferentiated and nerve growth factor-differentiated PC12 cells. Our data suggest that PC12 cells likely contain a single population of functional proton-gated channel-homomeric ASIC1a. It might be an ideal neuronal cell line for the study of physiological and potential pathological roles of this key subunit of ASICs.
Broad, Hybrid Capture–Based Next-Generation Sequencing Identifies Actionable Genomic Alterations in Lung Adenocarcinomas Otherwise Negative for Such Alterations by Other Genomic Testing Approaches (2015)

Broad, hybrid capture-based NGS identified actionable genomic alterations in 65% [95% confidence interval (CI), 48%-82%] of tumors from never or light smokers with lung cancers deemed without targetable genomic alterations by earlier extensive non-NGS testing. These findings support first-line profiling of lung adenocarcinomas using this approach as a more comprehensive and efficient strategy compared with non-NGS testing. See related commentary by McCutcheon and Giaccone, p. 3584.
First Principles Studies of Two Luminescent Molecular Quantum Clusters of Silver, Ag7(H2MSA)7 and Ag8(H2MSA)8, Based on Experimental Fluorescence Spectra (2011)

We report a joint experimental and theoretical study of two luminescent molecular quantum clusters of silver, Ag7(H2MSA)7 and Ag8(H2MSA)8 (H2MSA is the dicarboxylic acid of mercaptosuccinic acid in thiolate form). Global optimizations and property calculations are performed in the framework of density-functional theory (DFT-PBE) to search for the leading candidates with the lowest energy. The simulated excitation spectra of these two clusters are in good agreement with the corresponding experimental spectra. The presence of −RS–Ag–RS– as a stable motif has been confirmed in all of the lowest energy structures.
An Introduction to the Helium Ion Microscope (2007)

A new microscope has been developed that uses a beam of helium ions which is focused and scanned across the sample. In principle, and in its applications, it is similar to a traditional scanning electron microscope (SEM). However, the source technology, the sample interaction, and the contrast mechanisms are distinctly different. The helium ion source offers high brightness (4×109 A/cm2sr) and a small energy spread (ΔE/E∼3×10−5), and hence allows the beam to be focused to small probe sizes (as small as 0.25 nm). As the beam interacts with the sample, the beam penetrates relatively deeply before it diverges and hence there is a narrow sample interaction region near the surface. The helium beam generates secondary electrons, scattered helium atoms (ions and neutrals), and other detectable particles from which images can be generated or analysis can be performed.