gebhart/patrepeval huggingface dataset:
The Behavior of 3,6-Dimethylphthalic Anhydride in Friedel—Crafts and Grignard Condensations (1944)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Behavior of 3,6-Dimethylphthalic Anhydride in Friedel—Crafts and Grignard CondensationsMelvin S. Newman and Bradley T. LordCite this: J. Am. Chem. Soc. 1944, 66, 5, 733–735Publication Date (Print):May 1, 1944Publication History Published online1 May 2002Published inissue 1 May 1944https://pubs.acs.org/doi/10.1021/ja01233a021https://doi.org/10.1021/ja01233a021research-articleACS PublicationsRequest reuse permissionsArticle Views407Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Treatment of Advanced Leukemia in Mice with mRNA Engineered T Cells (2011)

Cytotoxic T lymphocytes (CTLs) modified with chimeric antigen receptors (CARs) for adoptive immunotherapy of hematologic malignancies are effective in preclinical models and are being tested in several clinical trials. Although CTLs bearing stably expressed CARs generated by integrating viral vectors are efficacious and have potential long-term persistence, this mechanism of CAR expression can potentially result in significant toxicity. T cells were electroporated with an optimized in vitro transcribed RNA encoding a CAR against CD19. These RNA CAR CTLs were then tested in vitro and in vivo for efficacy. We found that T cells expressing an anti-CD19 CAR introduced by electroporation with optimized mRNA were potent and specific killers of CD19 target cells. CD19 RNA CAR T cells given to immunodeficient mice bearing xenografted leukemia rapidly migrated to sites of disease and retained significant target-specific lytic activity. Unexpectedly, a single injection of CD19 RNA CAR T cells reduced disease burden within 1 day after administration, resulting in a significant prolongation of survival in an aggressive leukemia xenograft model. The surface expression of the RNA CARs may be titrated, giving T cells with potentially tunable levels of effector functions such as cytokine release and cytotoxicity. RNA CARs are a genetic engineering approach that should not be subject to genotoxicity, and they provide a platform for rapidly optimizing CAR design before proceeding to more costly and laborious stable expression systems.
Morocco - Broadband in Morocco : political will meets socio-economic reality (2011)

This report presents the broadband landscape in Morocco and the approach by which the country has advanced its information and communications technology sector over the past fifteen years. Despite being constrained by human development challenges and regional political uncertainty in today's Arab Spring, Morocco has emerged as a trailblazer in certain areas with particularly impressive mobile broadband results. Morocco's broadband share of total Internet subscribers was over 99 percent by 2008, exceeding that of its neighboring Arab states. The country boasts a quarter of households equipped with broadband, significantly higher than two percent in 2004. Almost half the country uses the Internet, demonstrating public demand in virtual networking and communicating via the World Wide Web. Overall, the report finds that three factors contributed to Morocco's success: prioritizing information and communications technology at an early stage so that subsequent planning could build on previous experiences; a youthful and eager population first getting online at the widespread cyber cafes and later wireless broadband; and expanding private competition and opting for an emphasis on technical requirements, innovation, quality, and access. Going beyond its initial broadband success and making broadband sustainable and transformational will be a challenge for Morocco. To deepen broadband access Morocco must find a way to deal with the social and economic reality of a lower-middle income country. This will require fresh and innovative solutions including more emphasis on bottom-up initiatives.
microRNA-17–92 Regulates IL-10 Production by Regulatory T Cells and Control of Experimental Autoimmune Encephalomyelitis (2013)

microRNAs (miRNA) are essential for regulatory T cell (Treg) function but little is known about the functional relevance of individual miRNA loci. We identified the miR-17-92 cluster as CD28 costimulation dependent, suggesting that it may be key for Treg development and function. Although overall immune homeostasis was maintained in mice with miR-17-92-deficient Tregs, expression of the miR-17-92 miRNA cluster was critical for Treg accumulation and function during an acute organ-specific autoimmune disease in vivo. Treg-specific loss of miR-17-92 expression resulted in exacerbated experimental autoimmune encephalitis and failure to establish clinical remission. Using peptide-MHC tetramers, we demonstrate that the miR-17-92 cluster was specifically required for the accumulation of activated Ag-specific Treg and for differentiation into IL-10-producing effector Treg.
Matrix Metalloproteinases Cleave Tissue Factor Pathway Inhibitor (2000)

The capacity of inflammatory cell-derived matrix metalloproteinases (MMPs) to cleave tissue factor pathway inhibitor (TFPI) and alter its activity was investigated. MMP-7 (matrilysin) rapidly cleaved TFPI to a major 35-kDa product. In contrast, MMP-1 (collagenase-1), MMP-9 (gelatinase B), and MMP-12 (macrophage elastase) cleaved TFPI into several fragments including the 35-kDa band. However, rates of cleavage were most rapid for MMP-7 and MMP-9. NH<sub>2</sub>-terminal amino acid sequencing revealed that MMP-12 cleaved TFPI at Lys<sup>20</sup>-Leu<sup>21</sup>(close to Kunitz I domain and producing a 35-kDa band), Arg<sup>83</sup>-Ile<sup>84</sup> (between Kunitz I and II domains), and Ser<sup>174</sup>-Thr<sup>175</sup> (between Kunitz II and III domains). MMP-7 and MMP-9 cleaved TFPI at Lys<sup>20</sup>-Leu<sup>21</sup> with additional COOH-terminal processing. These MMPs did not cleave tissue factor (TF), factor VII, and factor Xa. Proteolytic cleavage by MMP-1, MMP-7, MMP-9, and MMP-12 resulted in considerable loss of TFPI activity. These observations indicate specific cleavage of TFPI by MMPs, which broadens their substrate profile. Co-localization of MMPs, TF, and TFPI in atherosclerotic tissues suggests that release of MMPs from inflammatory cell leukocytes may effect TF-mediated coagulation.
Beating the maximum cooling limit with graded thermoelectric materials (2006)

The maximum cooling temperature of a uniform thermoelectric material is limited by its nondimensional figure of merit ZT. Conventionally cascaded stages or materials with a larger figure of merit are needed to obtain a higher cooling temperature. In this letter, the authors prove that the maximum cooling in an optimally graded single-stage device could be much larger than the value predicted based on the highest local ZT of the material. The cooling enhancement is attributed to the redistribution of the Joule heating and Peltier cooling profiles along the current and heat flow directions.
Guanosine analogs. Synthesis of nucleosides of certain 3-substituted 6-aminopyrazolo[3,4-d]pyrimidin-4(5H)-ones as potential immunotherapeutic agents (1990)

Several guanosine analogues were synthesized in the pyrazolo[3,4-d]pyrimidine ring system with various substituents at the 3-position. The new analogues prepared here include the CH3 (2-amino-3-methyl-1-beta-D-ribofuranosylpyrazolo[3,4-d]pyrimidin-4 (5H)-one, 13a), the phenyl (2-amino-3-phenyl-1-beta-D-ribofuranosylpyrazolo[3,4-d]pyrimidin-4 (5H)-one, 13b), and the NH2 (3,6-diamino-1-beta-D-ribofuranosylpyrazolo[3,4-d]pyrimidin-4(5H)- one, 17) substituted derivatives. These new agents, as well as several other 3-substituted derivatives including H, Br, OCH3, COOH, and oxo, were evaluated for their ability to potentiate certain murine immune functions relative to the known active agent 5-amino-3-beta-D-ribofuranosylthiazolo[4,5-d]pyrimidine-2,7(3H,6H) -dione (4, 7-thia-8-oxoguanosine). The biological evaluation included the (1) ex vivo determination of increased natural killer cell function and (2) in vivo antiviral protection against a lethal challenge of Semliki Forest virus. The 3-unsubstituted (5a) and the 3-bromo (5c) derivatives were found to be the most active immunopotentiators in this series.
Optimization of semiconductor–metal hybrid structures for application in magnetic-field sensors and read heads (2003)

Semiconductor–metal hybrid structures can exhibit a very large geometrical magnetoresistance effect, the so-called extraordinary magnetoresistance (EMR) effect. Using the finite element method, we study the EMR effect in rectangular semiconductor–metal hybrid structures and investigate the effects of material parameters and of device geometry. We find that the EMR device exhibits inverse scalability, i.e., the output characteristics improve with decreasing device width. This is promising for miniaturized magnetic-field sensors like, e.g., read heads. Using realistic device parameters, we predict an optimized performance as a sensor for a width-to-length ratio of 0.025.
Actin filament disassembly is a sufficient final trigger for exocytosis in nonexcitable cells. (1995)

Although the actin cytoskeleton has been implicated in vesicle trafficking, docking and fusion, its site of action and relation to the Ca(2+)-mediated activation of the docking and fusion machinery have not been elucidated. In this study, we examined the role of actin filaments in regulated exocytosis by introducing highly specific actin monomer-binding proteins, the beta-thymosins or a gelsolin fragment, into streptolysin O-permeabilized pancreatic acinar cells. These proteins had stimulatory and inhibitory effects. Low concentrations elicited rapid and robust exocytosis with a profile comparable to the initial phase of regulated exocytosis, but without raising [Ca2+], and even when [Ca2+] was clamped at low levels by EGTA. No additional cofactors were required. Direct visualization and quantitation of actin filaments showed that beta-thymosin, like agonists, induced actin depolymerization at the apical membrane where exocytosis occurs. Blocking actin depolymerization by phalloidin or neutralizing beta-thymosin by complexing with exogenous actin prevented exocytosis. These findings show that the cortical actin network acts as a dominant negative clamp which blocks constitutive exocytosis. In addition, actin filaments also have a positive role. High concentrations of the actin depolymerizing proteins inhibited all phases of exocytosis. The inhibition overrides stimulation by agonists and all downstream effectors tested, suggesting that exocytosis cannot occur without a minimal actin cytoskeletal structure.
Low-dose oral metronomic chemotherapy prevents mobilization of endothelial progenitor cells into the blood of cancer patients. (2009)

These observations provide evidence that conventional dose-dense chemotherapy leads to rebound EPC mobilization even when given with adjuvant intention, while low-dose metronomic scheduling of cytotoxic substances such as trofosfamide may sharply reduce EPC release into the circulation.
High-resolution tensor MR elastography for breast tumour detection (2000)

MR elastography is a novel imaging technique for the visualization of elastic properties of tissue. It is expected that this method will have diagnostic value for the clarification of suspicious breast lesions. Low-frequency mechanical waves are coupled into the tissue and visualized via an MR sequence which is phase-locked to the mechanical excitation. Commonly, elasticity is assumed to be isotropic and reconstruction is performed in only two dimensions. The technique is extended to three dimensions such that the entire symmetric elasticity tensor is assessed. This is achieved by measuring different phases of the mechanical wave during one oscillatory cycle. Thereby it is possible to provide information about the anisotropy of the elasticity tensor. Finite-element simulations as well as phantom experiments are performed to demonstrate the feasibility of the method. Initial clinical results of a breast carcinoma are presented. The analysis of the eigenvalues of the elasticity tensor support the hypothesis that breast carcinoma might exhibit an anisotropic elasticity distribution. The surrounding benign tissue appears isotropic. Thereby new and additional diagnostic information is provided which might help in distinguishing between benign and malignant breast diseases.
Concentrations of Bisphenol A, Bisphenol A Diglycidyl Ether, and Their Derivatives in Canned Foods in Japanese Markets (2008)

Bisphenol A (BPA), bisphenol A diglycidyl ether (BADGE), and their derivatives in 38 canned foods sold in Japan were measured using high-performance liquid chromatography-mass spectrometry (LC-MS) and LC-tandem mass spectrometry (LC-MS/MS). BPA, BADGE, BADGE.2H 2O, BADGE.HCl.H2O, BADGE.HCl, and BADGE.2HCl were 0-235.4, 0-3.4, 0-247.2, 0.2-196.4, 0-3.0, and 0-25.7 ng/g, respectively, which did not exceed acceptable daily intake for BPA and specific migration limit for BADGEs. BADGE was degraded by 58, 100, 46, and 58% in water (pH 7), 0.01 N HCl (pH 2), 0.01 N NaCl (pH 6.8), and 0.01 N NaCl with acetic acid (pH 2.5), respectively, when it was allowed to stand at 120 degrees C for 30 min. The prominent derivatives formed were BADGE.2H 2O and BADGE.HCl.H2O, which was formed not only in BADGE with added HCl but also in that with NaCl. Acetic acid accelerated the formation of both BADGE.2H2O and BADGE.HCl.H2O in NaCl. No BPA was detected in any simulation samples started from BADGE. The results suggest that BPA and BADGE are independently leached into canned foods and that BADGE is easily changed to more stable compounds such as BADGE.2H2O and BADGE.HCl.H2O by sterilization.
The Depression Anxiety Stress Scales (DASS): Normative data and latent structure in a large non‐clinical sample (2003)

The best fitting model (CFI =.93) of the latent structure of the DASS consisted of three correlated factors corresponding to the depression, anxiety and stress scales with correlated error permitted between items comprising the DASS subscales. Demographic variables had only very modest influences on DASS scores. The reliability of the DASS was excellent, and the measure possessed adequate convergent and discriminant validity Conclusions: The DASS is a reliable and valid measure of the constructs it was intended to assess. The utility of this measure for UK clinicians is enhanced by the provision of large sample normative data.
Specifically activated memory T cell subsets from cancer patients recognize and reject xenotransplanted autologous tumors (2004)

Bone marrow of breast cancer patients was found to contain CD8+ T cells specific for peptides derived from breast cancer–associated proteins MUC1 and Her-2/neu. Most of these cells had a central or effector memory phenotype (CD45RA–CD62L+ or CD45RA–CD62L–, respectively). To test their in vivo function, we separated bone marrow–derived CD45RA+ naive or CD45RA–CD45RO+ memory T cells, stimulated them with autologous dendritic cells pulsed with tumor lysate, and transferred them into NOD/SCID mice bearing autologous breast tumors and normal skin transplants. CD45RA– memory but not CD45RA+ naive T cells infiltrated autologous tumor but not skin tissues after the transfer. These tumor-infiltrating cells had a central or effector memory phenotype and produced perforin. Many of them expressed the P-selectin glycoprotein ligand 1 and were found around P-selectin+ tumor endothelium. Tumor infiltration included cluster formation in tumor tissue by memory T cells with cotransferred dendritic cells. It was associated with the induction of tumor cell apoptosis and significant tumor reduction. We thus demonstrate selective homing of memory T cells to human tumors and suggest that tumor rejection is based on the recognition of tumor-associated antigens on tumor cells and dendritic cells by autologous specifically activated central and effector memory T cells.
DEVELOPMENT AND APPLICATION OF HERPES SIMPLEX VIRUS VECTORS FOR HUMAN GENE THERAPY (1995)

Advances in understanding the molecular basis of human disease and the development of recombinant DNA methods is rapidly creating new means of disease diagnosis and treatment. Among the most revolutionary developments are technologies for transfer of therapeutic genes to the human body to treat both inherited and acquired disease. Gene therapy offers considerable promise for ameliorating otherwise intractable diseases such as immunopathological conditions, cancer, heart disease, and various metabolic and neurodegenerative syndromes. To fulfill this promise, more efficient and effective methods of gene delivery and appropriate gene expression must be developed. The lack of such techniques is currently the most significant impediment to the use of genetic therapy. Both viral and nonviral delivery systems are under development for specific gene-therapy applications. Herpes simplex virus (HSV) represents a novel vector system for gene delivery to the nervous system and other tissues. HSV is able to establish latency in nondividing neuronal cells in which genomes persist long-term but do not integrate or alter host-cell metabolism and that carry a promoter system uniquely capable of escaping repression that shuts off the expression of HSV-lytic genes during latency. This review examines efforts to create defective HSV vectors that are safe, noncytotoxic, and applicable to the treatment of cancer and diseases affecting peripheral nerves. Perhaps the most important use of HSV vectors will be for the treatment of neurodegenerative diseases of the brain, but additional studies are required to improve the design of promoters to ensure regulatable or effective levels of therapeutic gene expression.
The First Potent and Selective Inhibitors of the Glycine Transporter Type 2 (2001)

ADVERTISEMENT RETURN TO ISSUELetterNEXTThe First Potent and Selective Inhibitors of the Glycine Transporter Type 2Wilson L. Caulfield, Iain T. Collie, Rachel S. Dickins, Ola Epemolu, Ross McGuire, David R. Hill, Gillian McVey, J. Richard Morphy, Zoran Rankovic, and Hardy SundaramView Author Information Lead Discovery Unit, Organon Laboratories Ltd., Newhouse ML1 5SH, Scotland, U.K. Cite this: J. Med. Chem. 2001, 44, 17, 2679–2682Publication Date (Web):July 25, 2001Publication History Received30 November 2000Published online25 July 2001Published inissue 1 August 2001https://pubs.acs.org/doi/10.1021/jm0011272https://doi.org/10.1021/jm0011272rapid-communicationACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views1558Altmetric-Citations78LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Anions,Assays,Inhibition,Screening assays,Structure activity relationship Get e-Alerts
Speech and language therapy for aphasia following stroke (1999)

The main conclusion of this review is that speech and language therapy treatment for people with aphasia after a stroke has not been shown either to be clearly effective or clearly ineffective within a RCT. Decisions about the management of patients must therefore be based on other forms of evidence. Further research is required to find out if effectiveness of speech and language therapy for aphasic patients is effective. If researchers choose to do a trial, this must be large enough to have adequate statistical power, and be clearly reported.
CYP11A1 stimulates the hydroxylase activity of CYP11B1 in mitochondria of recombinant yeast in vivo and in vitro (2001)

In mammals, hydrocortisone synthesis from cholesterol is catalyzed by a set of five specialized enzymes, four of them belonging to the superfamily of cytochrome P-450 monooxygenases. A recombinant yeast expression system was recently developed for the CYP11B1 (P45011beta) enzyme, which performs the 11beta hydroxylation of steroids such as 11-deoxycortisol into hydrocortisone, one of the three mitochondrial cytochrome P-450 proteins involved in steroidogenesis in mammals. This heterologous system was used to test the potential interaction between CYP11B1 and CYP11A1 (P450scc), the mitochondrial cytochrome P-450 enzyme responsible for the side chain cleaving of cholesterol. Recombinant CYP11B1 and CYP11A1 were targeted to Saccharomyces cerevisiae mitochondria using the yeast cytochrome oxidase subunit 6 mitochondrial presequence fused to the mature form of the two proteins. In yeast, the presence of CYP11A1 appears to improve 11beta hydroxylase activity of CYP11B1 in vivo and in vitro. Fractionation experiments indicate the presence of the two proteins in the same membrane fractions, i.e. inner membrane and contact sites of mitochondria. Thus, yeast mitochondria provide interesting insights to study some molecular and cellular aspects of mammalian steroid synthesis. In particular, recombinant yeast should permit a better understanding of the mechanism permitting the synthesis of steroids (sex steroids, mineralocorticoids and glucocorticoids) with a minimal set of enzymes at physiological level, thus avoiding disease states.
Source digital camcorder identification using sensor photo response non-uniformity (2007)

Photo-response non-uniformity (PRNU) of digital sensors was recently proposed [1] as a unique identification fingerprint for digital cameras. The PRNU extracted from a specific image can be used to link it to the digital camera that took the image. Because digital camcorders use the same imaging sensors, in this paper, we extend this technique for identification of digital camcorders from video clips. We also investigate the problem of determining whether two video clips came from the same camcorder and the problem of whether two differently transcoded versions of one movie came from the same camcorder. The identification technique is a joint estimation and detection procedure consisting of two steps: (1) estimation of PRNUs from video clips using the Maximum Likelihood Estimator and (2) detecting the presence of PRNU using normalized cross-correlation. We anticipate this technology to be an essential tool for fighting piracy of motion pictures. Experimental results demonstrate the reliability and generality of our approach.