gebhart/patrepeval huggingface dataset:
Colonic Expression of MUC2, MUC5AC, and TFF1 in Inflammatory Bowel Disease in Children (2004)

The study confirms earlier observations that MUC2 is the major colonic mucin in IBD. It appears in two forms: mature MUC2 in goblet cells and immature MUC2 especially in secretory granules of cells that are not phenotypically goblet cells. MUC5AC and TFF1 expression in goblet cells is common in IBD and other inflammatory conditions of the colon. These changes may represent a nonspecific repair function of the colon cells to compensate for damage to barrier function.
Linearity and detection limit in the measurement of serum M-protein with the capillary zone electrophoresis system Capillarys ® (2005)

We studied the linearity and detection limits of the capillary zone electrophoresis system Capillarys in the measurement of serum monoclonal protein. Three monoclonal proteins with different isotypes and electrophoretic migrations were diluted with a hypo-gamma-globulinemic polyclonal serum pool. Mathematical linearity was observed for all monoclonal protein isotypes in the ranges studied without removing the polyclonal gamma-globulin background. Theoretical concentrations of 0.43, 0.89 and 0.33 g/L for monoclonal proteins immunoglobulin (Ig)G, IgA and IgM, respectively, gave a discernible spike by Capillarys, although they were measured as 0.76, 1.09 and 0.76g/L, respectively. We observed overestimation of monoclonal protein inversely correlated to concentrations below 15 g/L. All these limitations have to be taken into account when monitoring monoclonal proteins, because the loss of linearity and the protein background may hide an increase in concentration at low levels.
Formaldehyde Levels and the Factors Affecting These Levels in Homes in Perth, Western Australia (2002)

Formaldehyde is a highly irritant gas commonly encoun tered in indoor environments. Domestic levels of formal dehyde may vary with building practices and climate and the aim of the present study was to investigate formalde hyde levels in 185 homes in Perth, Australia. The formal dehyde was monitored using a validated passive sam pling technique. Levels were measured in four rooms of each house (living room, kitchen and large and small bedrooms). In 160 homes the monitoring was repeated 4-7 months later in a different season to the original measurement. It was found that within homes there was no significant difference in formaldehyde concentrations measured in the four rooms. For the first sampling peri od, the geometric mean of the average household con centration (average of the four rooms) for all homes was 22.8 ppb and ranged from 3.0 to 92.3 ppb. Only the age of the house (p &lt; 0.0001) and the season of monitoring (p &lt; 0.0001) were significant predictors of indoor levels, high er levels in newer homes and in homes monitored in summer. Mean concentrations were lower during the fol low-up monitoring, but only in houses that were less than 10 years old or in houses that were initially moni tored in summer. Overall formaldehyde concentrations were low and rarely exceeded the Australian guideline level of 100 ppb. There seemed to be good inter-room mixing of formaldehyde within homes. Concentrations were significantly affected by season and age of the house.
Confirmation of Duffy blood group antigen locus (FY) at 1q22→q23 by fluorescence in situ hybridization (1994)

The gene for the Duffy blood group antigen (FY) was previously assigned to the chromosome region 1q22-->q23 by linkage analysis. We confirm this localization by fluorescence in situ hybridization.
Le marché Rwanda à Addis-Abeba : migrants chinois et réseau alimentaire local (2019)

Le nombre croissant de migrants chinois en thiopie a cr un march de niche pour les produits alimentaires chinois, faisant du march Rwanda le seul march alimentaire urbain d'Addis-Abeba spcialis dans ce type de produits.Le caractre spcifique de ce march tient au fait que mme s'il rpond en priorit la demande des migrants trangers, la chane de valeur alimentaire et le rseau d'entreprises sont majoritairement locaux.Positionn dans un environnement commercial assez conventionnel au sein du secteur alimentaire thiopien, le rseau conomique local s'adapte activement aux besoins de la communaut des migrants et tend sa chane de valeur alimentaire. travers des interactions quotidiennes entre le rseau conomique local et les consommateurs de produits alimentaires chinois, des liens conviviaux se tissent et voluent dans le march et en dehors.Ces interactions contribuent au ragencement de l'espace socio-conomique du march et du paysage alimentaire de la ville.Cette tude du march Rwanda fournit des donnes empiriques pour le champ de recherche dynamique et en pleine diversification des rencontres entre la Chine et l'Afrique. MOTS-CLS : Espaces ordinaires, march
Circulating Adiponectin and Expression of Adiponectin Receptors in Human Skeletal Muscle: Associations with Metabolic Parameters and Insulin Resistance and Regulation by Physical Training (2006)

Adiponectin, AdipoR1, and AdipoR2 are all associated with body composition, insulin sensitivity, and metabolic parameters. Physical training increases circulating adiponectin and mRNA expression of its receptors in muscle, which may mediate the improvement of insulin resistance and the metabolic syndrome in response to exercise.
Topiramate for Treating Alcohol Dependence<SUBTITLE>A Randomized Controlled Trial</SUBTITLE> (2007)

clinicaltrials.gov Identifier: NCT00210925.
Relation of water soluble substances in fruits of sugar beet to speed of germination of sugar beet seeds (1965)

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A new approach to stereospecific synthesis of P-chiral phosphorothioates. Preparation of diastereomeric dithymidyl-(3′-5′) phosphorothioates (2004)

A new method for stereospecific synthesis of P-chiral phosphorothioates based on intramolecular nucleophile catalysis was developed.
A new approach for distinguishing cathepsin E and D activity in antigen‐processing organelles (2007)

Cathepsin E (CatE) and D (CatD) are the major aspartic proteinases in the endolysosomal pathway. They have similar specificity and therefore it is difficult to distinguish between them, as known substrates are not exclusively specific for one or the other. In this paper we present a substrate-based assay, which is highly relevant for immunological investigations because it detects both CatE and CatD in antigen-processing organelles. Therefore it could be used to study the involvement of these proteinases in protein degradation and the processing of invariant chain. An assay combining a new monospecific CatE antibody and the substrate, MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2[where MOCAc is (7-methoxycoumarin-4-yl)acetyl and Dnp is dinitrophenyl], is presented. This substrate is digested by both proteinases and therefore can be used to detect total aspartic proteinase activity in biological samples. After depletion of CatE by immunoprecipitation, the remaining activity is due to CatD, and the decrease in activity can be assigned to CatE. The activity of CatE and CatD in cytosolic, endosomal and lysosomal fractions of B cells, dendritic cells and human keratinocytes was determined. The data clearly indicate that CatE activity is mainly located in endosomal compartments, and that of CatD in lysosomal compartments. Hence this assay can also be used to characterize subcellular fractions using CatE as an endosomal marker, whereas CatD is a well-known lysosomal marker. The highest total aspartic proteinase activity was detected in dendritic cells, and the lowest in B cells. The assay presented exhibits a lower detection limit than common antibody-based methods without lacking the specificity.
Segmentation of the Aortic Dissection from CT Images Based on Spatial Continuity Prior Model (2016)

In order to improve the segmentation and reconstruction effect of the aortic dissection diagnostic equipment in hospital, we plan to develop a better three-dimensional reconstruction system of the aortic dissection to meet the requirements of the clinicians. This paper mainly introduces a series of preliminary work for the system: we utilize GVF snake model for descending aorta segmentation, extracting the aortic dissection membrane with the help of the Hessian matrix and the spatial continuity prior model based on Bayesian theory. We carried on the experiment in a series of continuous CT images, and the segmentation results were compared respectively with the manual segmentation results and the results without using the spatial continuity prior model. The experiment has proved that the spatial continuity prior model is effective for accurate segmentation of aortic dissection.
Molecular cloning of a ligand for the EPH-related receptor protein-tyrosine kinase Htk. (1995)

Htk is a receptor protein-tyrosine kinase that is related to the EPH subfamily of tyrosine kinases. The receptor has a wide tissue distribution including expression in several myeloid hematopoietic cell lines. Using an Htk-Fc fusion protein, a protein ligand for this receptor was expression cloned from the murine kidney mesangial cell line SV40MES 13. The Htk ligand cDNA encodes a transmembrane protein of 336 amino acids. Binding competition experiments demonstrated a Kd of 535 pM for binding of Htk-Fc to the Htk ligand. Incubation of 3T3 cells expressing Htk with COS-7 cells expressing the ligand resulted in tyrosine phosphorylation of Htk. The ligand, like its receptor, is widely expressed and may function in a variety of tissues. However, we localized hematopoietic expression of Htk to the monocytic lineage, suggesting that the ligand may play a role in differentiation and/or proliferation of these cells.
Printed circuit board embedded inductors for very high frequency Switch-Mode Power Supplies (2013)

The paper describes the design of three different structures for printed circuit board embedded inductors. Direct comparison of spirals, solenoids and toroids are made with regard to inductance, dc and ac resistance, electromagnetic field and design flexibility. First the equations for the impedances are given and an example of the achievable impedances are given. Prototypes are then made and measured. The differences between the three structures and the accuracy of the formulas are evaluated. Finite element simulations are used to investigate the magnetic field around the structure, in order to take possible electromagnetic interference problems into account, when the structures are compared. The simulated fields are verified through near field measurements performed on the prototypes. Finally deign flexibility are considered, both regarding scalability and design of the individual inductors and implementation in a complete design. At the end of the paper a summary of pros and cons of the three structures are listed.
A comparison of different initialization protocols to obtain statistically independent molecular dynamics simulations (2010)

We study how the results of molecular dynamics (MD) simulations are affected by various choices during the setup, e.g., the starting velocities, the solvation, the location of protons, the conformation of His, Asn, and Gln residues, the protonation and titration of His residues, and the treatment of alternative conformations. We estimate the binding affinity of ligands to four proteins calculated with the MM/GBSA method (molecular mechanics combined with a generalized Born and surface area solvation energy). For avidin and T4 lysozyme, all variations gave similar results within 2 kJ/mol. For factor Xa, differences in the solvation or in the selection of alternative conformations gave results that are significantly different from those of the other approaches by 4-6 kJ/mol, whereas for galectin-3, changes in the conformations, rotations, and protonation gave results that differed by 10 kJ/mol, but only if residues close to the binding site were modified. This shows that the results of MM/GBSA calculations are reasonably reproducible even if the MD simulations are set up with different software. Moreover, we show that the sampling of phase space can be enhanced by solvating the systems with different equilibrated water boxes, in addition to the common use of different starting velocities. If different conformations are available in the crystal structure, they should also be employed to enhance the sampling. Protonation, ionization, and conformations of Asn, Gln, and His may also be used to enhance sampling, but great effort should be spent to obtain as reliable predictions as possible close to the active site.
Response to Levodopa Treatment in Dopa-Responsive Dystonia (2001)

This pilot study suggests that retained dopamine storage in DRD may prolong the short-duration response and blur the distinction of the short- and long-duration responses. The decline in motor function in DRD on withdrawal of long-term levodopa therapy resembles that in Parkinson disease, suggesting that a long-duration response, if it exists in DRD, is unrelated to dopamine storage.
Messenger RNA expression of the immunosuppressive cytokine IL-10 in human gliomas. (1995)

Interleukin 10 (IL-10) was initially discovered on the basis of its ability to suppress cytokine synthesis. Additionally, it can exert immunosuppressive effects on a variety of cell types. Because patients with malignant gliomas present with a general impairment of the immune system, we investigated IL-10 expression in the glioma tissue. Because expression of IL-10 and IL-6 is associated in hematopoietic cells and IL-6 can act as an autocrine growth stimulator for glioblastoma cell lines, we looked in addition for a relationship between IL-10 and IL-6 expression. Using a quantitative reverse transcriptase polymerase chain reaction, IL-10 and IL-6 mRNA levels were determined in 37 glial tumors of different grades including 2 recurrencies, 3 specimens from normal brain tissue, and 3 glioblastoma cell lines. Expression of IL-10 mRNA was demonstrable in all tumors as well as in normal brain. High grade tumors and recurrent cases expressed significantly higher amounts of IL-10-specific mRNA compared with low grade tumors, whereas 2 of 3 cell lines showed only weak constitutive expression, mRNA for IL-6 was found in 86.5% of all gliomas with a correlation concerning the expression levels for both cytokines in 69% of gliomas. We suggest that IL-10 may contribute to the progression of astrocytomas by suppressing the patient's immune response, whereas IL-6 provides an additional growth advantage.
Osteopontin in chronic puromycin aminonucleoside nephrosis. (1997)

Increased expression of osteopontin (OPN) associated with interstitial monocyte infiltration has been demonstrated in the early phase of a variety of experimental renal diseases. Whether these changes occur in the chronic phase of progressive glomerular disease is unknown. Chronic puromycin aminonucleoside nephrosis (PAN) was induced in 16 rats by the injection of a single bolus of PA into the internal jugular vein, which results in a triphasic disease characterized by minimal glomerular change and marked proteinuria, peaking at about 10 to 14 d and subsiding by 28 d, followed by a quiescent 4-wk period of no or minimal proteinuria and then the development of progressive focal glomerulosclerosis (FGS) and increasing proteinuria. Fifteen rats injected similarly with normal saline served as controls. At 11 d after injection, PA rats demonstrated significantly greater urinary protein excretion (P = 0.0107), cortical tubular OPN expression (P = 0.0086), and intraglomerular (P = 0.0009) and interstitial (P = 0.0212) monocyte infiltration than did the controls. At 42 d, no significant differences between the two groups with respect to the above parameters were detected. At 98 d, PA rats had FGS and showed a definite trend to increased proteinuria, cortical tubular OPN, and intraglomerular monocyte infiltration. Although the cortical interstitial monocyte count was not elevated in PA rats compared with controls, there were significantly more monocytes around OPN-positive cortical tubules than around OPN-negative ones (P = 0.0011). Cortical tubular OPN expression correlated well with urinary protein excretion (r = 0.932, P < 0.0001), cortical tubular proliferating cell nuclear antigen (r = 0.796, P < 0.0001), and intraglomerular monocyte count (r = 0.552, P = 0.0013). The results are consistent with a monocyte chemoattractant role for OPN and suggest that OPN is upregulated in the chronic phase of PAN and that this increase in expression is a result of glomerular events.
Molecular quantification of residual disease in chronic myelogenous leukemia after bone marrow transplantation (1992)

Residual disease remains a major problem in the treatment of human neoplasia. To effectively monitor minimal leukemic activity after bone marrow transplantation (BMT), we used a competitive polymerase chain reaction (PCR) amplification technique to quantify expression of the characteristic bcr-abl fusion message in patients with chronic myelogenous leukemia (CML). Quantitative results were obtained between the 0.001% and 0.1% level in control experiments. This represents a significant advantage over cytogenetic and Southern blotting techniques routinely used to diagnose CML, which may not be sensitive below the 1% level. To illustrate the potential clinical usefulness of the quantitative PCR strategy, we compared results of bcr-abl messenger RNA expression with those obtained using cytogenetic and Southern blotting techniques, in a study of consecutive BM and peripheral blood (PB) samples from two CML patients at high risk for relapse after BMT. One patient received a syngeneic transplant during the chronic phase of the disease and relapse was apparent at the molecular level 4.5 months after BMT, while the patient was in complete clinical remission. The second patient was treated with an allogeneic BMT during the accelerated phase of the disease. A slow, but progressive decrease in bcr-abl expression was observed during the first 12 months after BMT, and expression was undetectable thereafter. Our results indicate that the competitive PCR technique can be used to monitor disease activity in patients at high risk of relapse, while the patients are in complete clinical remission, which should facilitate the early detection of relapse or the identification of progressive disappearance of leukemic activity. The approach used may serve as a model for the study of residual disease in an increasing number of other hematologic malignancies that express cancer-specific RNAs.
Differential Expression of Translational Elements by Life Cycle Variants ofCoxiella burnetii (1999)

Coxiella burnetii replicates as distinct morphological forms, which may allow potential life cycle variants to survive the harsh environment of the phagolysosome. Monoclonal antibodies (MAbs) were compared by Western blotting for reactivity with large cell variant (LCV) and small cell variant (SCV) antigens to characterize proteins differentially expressed by C. burnetii. MAb NM7.3 reacted with a approximately 32-kDa LCV-upregulated antigen, and MAb NM183 reacted with a approximately 45-kDa LCV-specific antigen. MAb NM7.3 was used to screen a lambdaZapII C. burnetii DNA expression library, and an immunoreactive clone was identified with sequence similarity to the Escherichia coli tsf gene, which encodes elongation factor Ts (EF-Ts). Since a similar screen with MAb NM183 did not identify immunoreactive clones, an alternate strategy was devised to clone the reactive antigen based on observations of cross-reactivity with the 45-kDa elongation factor Tu (EF-Tu) protein from Chlamydia trachomatis. The highly conserved nature of EF-Tu among eubacteria allowed PCR amplification of a tuf gene fragment (encoding approximately 95% of the predicted EF-Tu open reading frame) from C. burnetii using degenerate primers. The product of the cloned tuf gene fragment reacted with MAb NM183 in Western blot analysis, confirming the identity of the 45-kDa LCV-specific antigen. Identification of two proteins differentially expressed by C. burnetii, EF-Tu and EF-Ts, both essential components of the translational machinery of the cell, supports the hypothesis that LCVs are metabolically more active than SCVs.
Phenotypic characterization of the dwarf-4 mutant of lettuce (1994)

The single recessive mutant dwarf-4 (d-4) of lettuce (Lactuca sativa L.) was selected as a potential genetic source of resistance to premature stem elongation (bolting). This study was undertaken to define the mutant phenotype and to characterize its reaction to applied gibberellin (GA). Scanning electron micrographs of mutant and wild-type achenes revealed surface and subsurface aberrations in d-4, suggesting abnormal development. Observed mutant irregularities included (i) abnormal achene formation causing irregularly shaped seeds, (ii) delayed and irregular germination, (iii) delayed but normal leaf growth and leaf area, (iv) 66% reduction in stem height, (v) 50% reduction in shoot dry weight, (vi) 0.45% viable pollen formation, and (vii) 98.9% self-sterility. Applications of GA 3 did not affect the delay in growth, floral sterility, or abnormal achene shape but did increase stem height and shoot dry weight to wild-type levels at maturity. Hand pollinations were able to decrease self-sterility to 93.6%. Component analysis of mutant and wild-type achenes revealed d-4 had 32% lower fatty acid levels. There were no differences in carbohydrates, cell wall constituents, and moisture levels, although higher levels of protein and inorganic compounds were found. The observed d-4 phenotype suggests that its genetic lesion is not directly tied to irregularities of gibberellin biosynthesis but has some other genetic basis. Key words: Lactuca sativa, SEM, germination, stem elongation, sterility.
Treatment of Patients With Low-Grade B-Cell Lymphoma With the Combination of Chimeric Anti-CD20 Monoclonal Antibody and CHOP Chemotherapy (1999)

This is the first report demonstrating the safety and efficacy of Rituxan anti-CD20 chimeric antibody in combination with standard-dose systemic chemotherapy in the treatment of indolent B-cell lymphoma. The clinical responses suggest an additive therapeutic benefit for the combination with no significant added toxicity. The conversion of bcl-2 from positive to negative by PCR in blood and/or marrow suggests possible clearing of minimal residual disease not previously demonstrated by CHOP chemotherapy alone.