gebhart/patrepeval huggingface dataset:
Structure and expression of a tandem gene pair in Leishmania donovani that encodes a protein structurally homologous to eucaryotic cation-transporting ATPases. (1987)

An oligonucleotide probe was used to clone a cation-transporting ATPase gene from the genome of Leishmania donovani. The nucleotide sequence of the gene contained a 2,922-base-pair open reading frame that was predicted to encode a 107,406-dalton protein composed of 974 amino acids. The predicted L. donovani protein contained all the structural and functional domains expected to be present in a cation-transporting ATPase of the aspartyl phosphate class. The nucleotide sequence encoding the ATPase gene was duplicated in tandem in the parasite genome. Partial sequenation of the second member of the tandem repeat, which lay 2 kilobase pairs downstream of the ATPase gene, indicated that it was either identical to the first gene or very closely related to it. RNA homologous to either the ATPase gene or its adjacent relative was 5 kilobases in size and was approximately equally abundant in both promastigote and amastigote forms of the organism.
Low expression of a few genes indicates good prognosis in estrogen receptor positive breast cancer (2009)

A breast cancer subtype defined by low expression of a few genes, using a minimum of statistical modeling, has significant prognostic power. This test is of potential clinical benefit in deciding a course of treatment for early stage breast cancer patients.
Holst Filtration Apparatus for Van Soest Detergent Fiber Analyses (1973)

Abstract New filtration equipment was developed to improve the acid-detergent method of fiber fractionation. This apparatus incorporates a vacuum manifold with an air metering device that provides the operator very precise control over the partial vacuum, permitting a very gradual change in vacuum level as needed for proper filtration. The partial pressure is provided by a water aspirator. A hot water jacket permits a continuous flow of hot water around the sample during the filtering process to keep the sample hot. This speeds filtering on practically all samples and is essential for reliable results on samples high in carbohydrates and salts. As a result of this new filtration equipment we do not need asbestos for a filter aid. This eliminates the need for duplicate acid-detergent fiber analyses when using 72% H2SO4 for the cellulose and lignin determinations. Six independent analyses were performed on each of 9 samples (3 feces, 3 orts, and 3 feeds) for the determination of acid-detergent fiber and its constituents. The average maximum differences for these analyses indicate that this filtration apparatus and the method are reproducible and have reduced the filtration time from several hours, on some samples, to 15 min.
Potent non-peptide fibrinogen receptor antagonists which present an alternative pharmacophore (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPotent non-peptide fibrinogen receptor antagonists which present an alternative pharmacophoreThomas W. Ku, William H. Miller, William E. Bondinell, Karl F. Erhard, Richard M. Keenan, Andrew J. Nichols, Catherine E. Peishoff, James M. Samanen, Angela S. Wong, and William F. HuffmanCite this: J. Med. Chem. 1995, 38, 1, 9–12Publication Date (Print):January 1, 1995Publication History Published online1 May 2002Published inissue 1 January 1995https://pubs.acs.org/doi/10.1021/jm00001a003https://doi.org/10.1021/jm00001a003research-articleACS PublicationsRequest reuse permissionsArticle Views160Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Botanical dietary supplement use in peri- and postmenopausal women (2003)

A high percentage of women at UIC clinics were using multiple BDS. The respondents believed that these products were both safe and effective for the treatment of common ailments. Concomitant BDS use with prescription and over-the-counter medications was commonplace, often without a physician's knowledge. Consumer education about the possible benefits and risks associated with BDS use is urgently needed.
Concept of autoimmunity following spinal cord injury: Possible roles for T lymphocytes in the traumatized central nervous system (1996)

The effect of immunological activation on the neuropathologic sequelae and neurologic outcome from spinal cord injury is unclear. Similar to models of neuroinflammatory disease (e.g., experimental autoimmune encephalomyelitis; EAE), injury to the spinal cord precipitates the activation of resident microglia and the recruitment of circulating inflammatory cells (e.g., macrophages and lymphocytes). In EAE, these cells are known to cause tissue damage and loss of neurological function via autoimmune reactions to myelin proteins. The role these cells play in the pathology of traumatic injury to the spinal cord has not been clarified. In this review, data are presented that indicate that T cells isolated from spinal-injured rats are capable of causing neurologic deficits and histopathologic changes similar to EAE when injected intravenously into naive animals. These data are consistent with the concept of trauma-induced autoimmune reactions. However, disease transfer was only possible when T cells were obtained from animals at 1 week post-injury. Thus, the encephalitogenic T-cell repertoire appears to be rapidly regulated. It is possible that trauma-induced autoimmunity evolves into a mechanism by which the autoreactive repertoire regulates ongoing central nervous system (CNS) immunologic responses. Similar immunoregulatory networks have been proposed in EAE and are discussed here in the context of CNS trauma and neurodegenerative disease.
Gut Hormones as Mediators of Appetite and Weight Loss After Roux-en-Y Gastric Bypass (2007)

The attenuated appetite after gastric bypass is associated with elevated PYY and GLP-1 concentrations, and appetite returns when the release of gut hormones is inhibited. The results suggest a role for gut hormones in the mechanism of weight loss after gastric bypass and may have implications for the treatment of obesity.
Learning Multi-domain Convolutional Neural Networks for Visual Tracking (2016)

We propose a novel visual tracking algorithm based on the representations from a discriminatively trained Convolutional Neural Network (CNN). Our algorithm pretrains a CNN using a large set of videos with tracking ground-truths to obtain a generic target representation. Our network is composed of shared layers and multiple branches of domain-specific layers, where domains correspond to individual training sequences and each branch is responsible for binary classification to identify target in each domain. We train each domain in the network iteratively to obtain generic target representations in the shared layers. When tracking a target in a new sequence, we construct a new network by combining the shared layers in the pretrained CNN with a new binary classification layer, which is updated online. Online tracking is performed by evaluating the candidate windows randomly sampled around the previous target state. The proposed algorithm illustrates outstanding performance in existing tracking benchmarks.
The phylogeny of Staphylococcus aureus – which genes make the best intra-species markers? (2006)

The ability to make informed decisions on the suitability of alternative marker loci is central for population and epidemiological investigations. This issue was addressed using Staphylococcus aureus as a model population by generating nucleotide sequence data from 33 gene fragments in a representative sample of 30 strains. Supplementing the data with pre-existing multilocus sequence typing data, an intra-species tree based on approximately 17.8 kb of sequence was reconstructed and the goodness of fit of each individual gene tree was computed. No strong association was noted between gene function per se and phylogenetic reliability, but it is suggested that candidate loci should possess at least the average degree of nucleotide diversity for all genes in the genome. In the case of S. aureus this threshold is >1 % mean pairwise diversity.
Experience in Autologous and Allogeneic Cord Blood Banking (1996)

Transplantation and gene therapy offer the hope of treating thousands of individuals with various malignancies and genetic disorders. However, if this approach is to become a reality, there must be readily available sources of suitable HLA-A typed stem cells. Cord blood has been demonstrated to be a suitable source of hematopoietic stem cells that can be used in place of bone marrow stem cells for transplant. However, to implement the widespread clinical use of cord blood, it is necessary to establish both autologous and allogeneic cord blood banks from which stem cell specimens can be obtained. These cord blood banks must be large in size, reflect the ethnic diversity of the patient populations in need, and be financially and logistically feasible. At the Cord Blood Stem Cell Bank at the University of Arizona, we have addressed many of these issues and have taken the necessary steps to create both autologous and allogeneic cord blood banks. We have developed simple, efficient, and reproducible methods for cord blood collection that can be used by any physician without need for previous training. Further, we have developed methodologies to process the cord blood specimen so that samples can be stored in cryovials. This approach to cord blood banking allows for the storage of more than 10,000 independent cord blood specimens in a single liquid nitrogen freezer, reducing the costs and physical space requirements for such a facility. We also have developed the use of electronic integration of laboratory worksheets and controlled rate freezers that permit high laboratory throughput with reduced problems of reproducibility, data entry, and management errors. In terms of allogeneic cord blood banking, we have based our bank on several important assumptions regarding HLA haplotype frequency, ethnic diversity, and the need for less than perfect HLA matching between donor and recipient. These assumptions have allowed us to begin to create an allogeneic cord blood bank that can rapidly attain the size needed for clinical utility without exorbitant costs or time involved. Results of our efforts in autologous and allogeneic cord blood banking are presented (including factors that positively and negatively affect cord blood collection and banking).
Micronetwork-based integration for SOCs (2001)

In this paper, we describe the concept of using an on-chip network as the fundamental communication architecture for a complex SOC design. We describe some of the salient features of a MicroNetwork that we have implemented, and introduce an automated development environment that makes MicroNetwork-based design productive. Finally, we show how one would use our MicroNetwork to integrate an SOC, and provide a general description of several completed MicroNetwork-based designs.
Shrink‐Film Configurable Multiscale Wrinkles for Functional Alignment of Human Embryonic Stem Cells and their Cardiac Derivatives (2011)

A biomimetic substrate for cell-culture is fabricated by plasma treatment of a prestressed thermoplastic shrink film to create tunable multiscaled alignment "wrinkles". Using this substrate, the functional alignment of human embryonic stem cell derived cardiomyocytes is demonstrated.
In situ detection of enterocytic apoptosis in normal colonic mucosa and in familial adenomatous polyposis. (1995)

Physiological regeneration of colonic epithelium entails proliferation at the crypt base and cell loss by shedding or cell death. The aim of this study was to localise and assess the rate of apoptosis in normal and neoplastic colonic epithelium with respect to zones of proliferation. Familial adenomatous polyposis (FAP) was chosen as a model to study neoplastic transformation of colonic mucosa at an early stage. Apoptotic cells were detected in situ by TdT-mediated biotin-dUTP nick end labelling (TUNEL) in parallel to cells in cycle determined by Ki-67 immunohistochemistry using the monoclonal antibody MiB-1. By detection of genomic fragmentation, two different patterns of enterocytic apoptosis emerged: (a) apoptotic bodies being engulfed by adjacent epithelial cells, and (b) apoptotic cells with only subtle morphological changes being extruded into the gut lumen. The engulfment pattern was seen predominantly in the crypts of the normal colonic mucosa and, although very rare, was clearly confined to the basal proliferation compartment. The extrusion pattern was restricted to the luminal mucosal surface. Adenomas of FAP showed highly increased numbers of apoptotic bodies, which were scattered throughout the transformed mucosa. Both patterns of apoptosis were topographically intermingled although the extrusion pattern prevailed at the luminal adenoma surfaces. Whereas cells in cycle were somewhat more numerous in the upper parts of the crypts, apoptosis occurred with increased frequency at sites beneath the proliferation maximum suggesting an inverted direction of epithelial cell migration in adenomas. These results suggest two distinct routes towards enterocytic apoptosis in the colonic mucosa leading to engulfment or extrusion of dying cells. Adenomatous transformation of colon epithelium is associated with a considerable increase of the cellular turnover rate and with a severe disturbance of the microtopographical localisation of birth and death of enterocytes.
Medical Applications of Space Light-Emitting Diode Technology--Space Station and Beyond (1999)

Space light-emitting diode (LED) technology has provided medicine with a new tool capable of delivering light deep into tissues of the body, at wavelengths which are biologically optimal for cancer treatment and wound healing. This LED technology has already flown on Space Shuttle missions, and shows promise for wound healing applications of benefit to Space Station astronauts.
Resistant Fits for Some Commonly Used Logistic Models with Medical Applications (1982)

Logistic regression-type models are used in many applications. Some examples include the classical dose-response experiment, prospective and retrospective studies of disease incidence (with and without matching), and the analysis of ordinal data. In most instances, the model is fitted by the method of maximum likelihood, which, like least squares, is sensitive to atypical observations. An alternative to maximum likelihood is proposed and illustrated by examples.
Bifunctional Transition Metal Based Molecular Catalysts for Asymmetric Syntheses (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Translational coupling in Escherichia coli of a heterologous Bacillus subtilis-Escherichia coli gene fusion (1986)

The efficient expression in Escherichia coli of the Tn9-derived chloramphenicol acetyltransferase (EC 2.3.1.28) gene fused distal to the promoter and N terminus of the Bacillus subtilis aprA gene was dependent on the initiation of translation from the ribosome-binding site in the aprA gene.
Time-Optimal Negative Input Shapers (1997)

Input shaping reduces residual vibration in computer controlled machines by convolving a sequence of impulses with a desired system command. The resulting shaped input is then used to drive the system. The impulse sequence has traditionally contained only positively valued impulses. However, when the impulses are allowed to have negative amplitudes, the rise time can be improved. Unfortunately, excitation of unmodeled high modes and overcurrenting of the actuators may accompany the improved rise time. Solutions to the problem of high-mode excitation and overcurrenting are presented. Furthermore, a simple look-up method is presented that facilitates the design of negative input shapers. The performance of negative shapers is evaluated experimentally on two systems; one driven by a piezo actuator and the other equipped with DC motors.
Engineering the world (2009)

This publication contains reprint articles for which IEEE does not hold copyright. Full text is not available on IEEE Xplore for these articles.
Digital predistortion for power amplifiers with memory effects (2011)

Power amplifier is essential component in communication systems and it's inherently nonlinear. This essay mainly investigate a based on Hammerstein predistorter, a memory polynomial predistorter. The Hammerstein predistorter is designed specifically for power amplifiers that can be modeled as a Wiener system. The memory polynomial predistorter can correct both the nonlinear distortions and the linear frequency response that may exist in the power amplifier.
Analgesic Effects of Ketamine Administered in Subdissociative Doses (1971)

SADOVE, MAX S. M.D.; SHULMAN, MORTON M.D.; HATANO, SHIGERU M.D., Ph.D.; FEVOLD, NANCY C.R.N.A. Author Information
Linear gene fusions of antibody fragments with streptavidin can be linked to biotin labelled secondary molecules to form bispecific reagents (1997)

Monomeric single chain antibody (scFv) fragments lack both the avidity of the bivalent IgG, or (Fab')2 fragment, and the effector functions conferred by the Fc domain. For certain diagnostic or therapeutic applications it may be desirable to link these molecules to other proteins, antibodies, enzymes or peptide ligands, and chemical or recombinant methods have been developed to produce many of these crosslinked reagents. One approach has been to link an antibody fragment to streptavidin which can bind a second biotinylated molecule to create a higher affinity, bifunctional or bispecific molecule. To demonstrate the applicability of this technology, an anti-neuraminidase NC10 scFv-streptavidin fusion was expressed in E. coli and the product was refolded and purified to homogeneity from 6 M guanidine hydrochloride. Analysis in a BIAcore biosensor showed that the NC10 scFv moiety reacted with immobilised neuraminidase and that the core streptavidin moiety was able to bind biotinylated anti-ferritin Fab' to produce a new model bispecific reagent which bound ferritin. Conceptually, this design principle can be applied to the creation of useful diagnostic and possibly therapeutic molecules.
Interaction of epithelial ion channels with the actin-based cytoskeleton (2006)

The interaction of ion channels with the actin-based cytoskeleton in epithelial cells not only maintains the polarized expression of ion channels within specific membrane domains, it also functions in the intracellular trafficking and regulation of channel activity. Initial evidence supporting an interaction between epithelial ion channels and the actin-based cytoskeleton came from patch-clamp studies examining the effects of cytochalasins on channel activity. Cytochalasins were shown to either activate or inactivate epithelial ion channels. An interaction between the actin-based cytoskeleton and epithelial ion channels was further supported by the fact that the addition of monomeric or filamentous actin to excised patches had an effect on channel activity comparable to that of cytochalasins. Through the recent application of molecular and proteomic approaches, we now know that the interactions between epithelial ion channels and actin can either be direct or indirect, the latter being mediated through scaffolding or actin-binding proteins that serve as links between the channels and the actin-based cytoskeleton. This review discusses recent advances in our understanding of the interactions between epithelial ion channels and the actin-based cytoskeleton, and the roles these interactions play in regulating the cell surface expression, activity, and intracellular trafficking of epithelial ion channels.
Formation of Rotaxane Dendrimers by Supramolecular Click Chemistry (2007)

Department of Chemistry and Department of Medical Bioscience, Dong-A University, Busan 604-714, Korea *E-mail: jlee@donga.ac.kr Department of Chemical & Biochemical Engineering, Dongguk University, Seoul 100-715, Korea National Creative Research Initiative Center for Smart Supramolecules, and Department of Chemistry, Pohang University of Science and Technology, Pohang 790-784, Korea. *E-mail: kkim@postech.ac.kr Received July 5, 2007
New Anticoagulant Prompts Bad Blood Between Partners (1996)

When scientists from Yale University and Corvas International Inc. joined forces in 1993, it seemed like a perfect marriage bound by a strong common interest. The Yale group wanted to develop a vaccine to protect people infected by a bloodsucking parasite, the hookworm; and the company was hoping to isolate a hookworm protein that could compete in the lucrative anticoagulant drug market. The birth announcement this month—a journal report of the amino acid sequences of hookworm anticoagulant proteins—should have been a joyous occasion. It was not. The partners have separated, and the rights to one molecule may become the object of a nasty custody battle. These wrangles seem to be happening more often as academic scientists increasingly work with for-profit corporations, and one observer calls them “special commercialization projects from hell.”
Long-term renewal of hair follicles from clonogenic multipotent stem cells (2005)

Adult stem cells are essential for tissue renewal, regeneration, and repair, and their expansion in culture is of paramount importance for regenerative medicine. Using the whisker follicle of the rat as a model system, we demonstrate that (i) clonogenicity is an intrinsic property of the adult stem cells of the hair follicle; (ii) after cultivation for >140 doublings, these stem cells, transplanted to the dermo-epidermal junction of newborn mouse skin, form part or all of the developing follicles; (iii) the stem cells incorporated into follicles are multipotent, because they generate all of the lineages of the hair follicle and sebaceous gland; (iv) thousands of hair follicles can be generated from the progeny of a single cultivated stem cell; (v) cultured stem cells express the self-renewal genes Bmi1 and Zfp145;(vi) several stem cells participate in the formation of a single hair bulb; and (vii) there are many more stem cells in whisker follicles than could be anticipated from label-retaining experiments.
Selected cyclic dipeptides inhibit cancer cell growth and induce apoptosis in HT-29 colon cancer cells. (2004)

Our findings demonstrate that cyclo(Phe-Pro) inhibits the growth of HT-29, MCF-7 and HeLa cells and induces apoptosis in HT-29 colon cancer cells, suggesting a potential antitumour activity.