gebhart/patrepeval huggingface dataset:
A nicotinic acetylcholine receptor ligand of unique specificity, alpha-conotoxin ImI (1994)

We report the isolation, characterization, and total synthesis of a small peptide ligand for nicotinic acetylcholine receptors (nAChRs). It is highly active against the neuromuscular receptor in frog but not in mice. In contrast, it induces seizures when injected centrally in mice and rats, suggesting that it may target neuronal nAChRs in mammals. Although such receptors may be important in both normal cognition and the pathophysiology of several neuropsychiatric disorders, there are few ligands to discriminate between the multiple receptor subtypes. The new peptide is a highly divergent alpha-conotoxin from the snail Conus imperialis, which preys on polychaete worms. In this article, the purification, structural analysis, synthesis, and preliminary physiological characterization of alpha-conotoxin ImI (alpha-CTx-ImI) are reported. The sequence of the peptide is: Gly-Cys-Cys-Ser-Asp-Pro-Arg-Cys-Ala-Trp-Arg-Cys-NH2. The peptide shows striking sequence differences from all alpha-conotoxins of fish-hunting Conus, but its disulfide-bridging is similar: [2-8; 3-12]. We suggest that cone venoms may provide an array of ligands with selectivity for various neuronal nAChR subtypes.
Advances in the laboratory culture of octopuses for biomedical research. (1985)

Five species of Octopus were cultured in pilot, large-scale 2,600 liter circulating seawater systems. Improvements in system design, water management and culture methodology were described. These five species all produced large eggs and correspondingly large hatchlings that had no planktonic or larval stage and thus were easier to culture. Octopuses grew well only when fed live marine crustaceans, fishes and other molluscs. Growth occurred as a 4-7% increase in body weight per day during the early exponential growth phase and 2-4% during the latter 1/2 to 3/4 of the life cycle, which ranged from 6-15 months depending upon species. All species reproduced in captivity. Survival was 70-80% when octopuses were reared in individual containers, but in group culture survival dropped to as low as 40% by the adult stage. Causes of mortality were species-specific and included hatchling abnormalities, escapes, aggression, cannibalism, disease, senescence and laboratory accidents. Octopus bimaculoides showed superior qualities for laboratory culture. The future potential of providing American scientists with laboratory-cultured octopuses was discussed along with their uses in biomedical research.
Repulsive guidance molecule/neogenin: a novel ligand‐receptor system playing multiple roles in neural development (2004)

The repulsive guidance molecule (RGM) is a membrane-bound protein originally isolated as an axon guidance molecule in the visual system. Recently, the transmembrane protein, neogenin, has been identified as the RGM receptor. In vitro analysis with retinal explants showed that RGM repels temporal retinal axons and collapses their growth cones through neogenin-mediated signaling. However, RGM and neogenin are also broadly expressed at the early embryonic stage, suggesting that they do not only control the guidance of visual axons. Gene expression perturbation experiments in chick embryos showed that neogenin induces cell death, and its ligand, RGM, blocks the pro-apoptotic activity of neogenin. Thus, RGM/neogenin is a novel dependence ligand/receptor couple as well as an axon guidance molecular complex.
Industrial enzymes and their applications (1998)

General Characteristics of Technical Enzymes. Description of Enzymes. Carrier-Bound Enzymes: Methods of Immobilization. Application of Technical Enzyme Preparations. Legal Considerations. Economic Considerations for the Use of Technical Enzymes. The Market for Technical Enzyme Preparations. Appendices. Index.
Encyclopedic Dictionary of Applied Geophysics (2002)

The fourth edition of SEG's best seller is a valuable, comprehensive reference that is a must for every geophysicist, geologist, explorationist, engineer, energy adviser, economist, editor, and student involved in the field. Hundreds of terms have been added since publication of the third edition in 1991, reflecting rapid evolution of the science, especially in the areas of engineering and production problems, 3D (including multicomponent) acquisition and processing, visualization, S- and converted waves, interpretation, anisotropy, AVO, geostatistics, geohazards, neural networks, tomography, downhole measurements, horizontal drilling, and deepwater work. Definitions of hundreds of other terms have been updated. The dictionary's title has been modified slightly to reflect growth in application of geophysical methods, with the word Applied replacing the word Exploration. The dictionary includes a guide to pronunciation and a list of reference figures and tables. A CD containing the dictionary in searchable PDF format also is included.
Synthesis of bicyclic cyclopentanols by photoreductive cyclization of .delta.,.epsilon.-unsaturated ketones (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of bicyclic cyclopentanols by photoreductive cyclization of .delta.,.epsilon.-unsaturated ketonesD. Belotti, J. Cossy, J. P. Pete, and C. PortellaCite this: J. Org. Chem. 1986, 51, 22, 4196–4200Publication Date (Print):October 1, 1986Publication History Published online1 May 2002Published inissue 1 October 1986https://pubs.acs.org/doi/10.1021/jo00372a018https://doi.org/10.1021/jo00372a018research-articleACS PublicationsRequest reuse permissionsArticle Views1256Altmetric-Citations99LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Sleep effects on ambulatory blood pressure measurements in pregnant women (2001)

The aim of this study was to investigate the effect of the definition of daytime and nighttime on ambulatory blood pressure (ABP) in pregnancy. To determine the prevalence of a <10% decrease in mean arterial pressure with sleep (nondipper) in pregnancy and the consistency of nondipper status throughout pregnancy. In a prospective, longitudinal study, 102 pregnant woman underwent 24-h ABP monitoring and recorded sleep patterns at < or = 14, 19 to 22, 27 to 30, 35 to 37 weeks' gestation and 5 to 9 weeks' postpartum. Nighttime was defined by arbitrary hours or actual periods of sleep. Ambulatory blood pressure measurements using the different definitions of nighttime were compared in the total cohort and individual women. Nondipper status of each woman was determined throughout pregnancy and postpartum. At 35 to 37 weeks' gestation, 19% of women napped during the day and 20% were awake during the night. Throughout pregnancy, mean day and night systolic and diastolic ABP measurements were similar whether daytime and nighttime were defined by arbitrary hours or by sleep periods. In individuals, the use of arbitrary hours (day 07:00 to 21:59, night 22:00 to 06:59) compared to sleep periods to define day and night resulted in differences in daytime systolic (SBP) and diastolic blood pressure (DBP) of up to 4 and 5 mm Hg, respectively. Similarly there were individual differences in nighttime SBP up to 10 mm Hg and DBP up to 11 mm Hg. A third of women were nondippers at least once during pregnancy, but only two women were consistent nondippers. The different definitions of day and night did not change group ABP measurements, but resulted in significant variation in ABP measurements in individual pregnant women. Nondippers were common and nondipper status frequently changed during pregnancy.
A superfamily of Arabidopsis thaliana retrotransposons. (1991)

We describe a superfamily of Arabidopsis thaliana retrotransposable elements that consists of at least ten related families designated Ta1-Ta10. The Ta1 family has been described previously. Two genomic clones representing the Ta2 and Ta3 elements were isolated from an A. thaliana (race Landsberg erecta) lambda library using sequences derived from the reverse transcriptase region of Ta1 as hybridization probes. Nucleotide sequence analysis showed that the Ta1, Ta2 and Ta3 families share greater than 75% amino acid identity in pairwise comparisons of their reverse transcriptase and RNase H genes. In addition to Ta1, Ta2 and Ta3, we identified seven other related retrotransposon families in Landsberg erecta, Ta4-Ta10, using degenerate primers and the polymerase chain reaction to amplify a highly conserved region of retrotransposon-encoded reverse transcriptase. One to two copies of elements Ta2-Ta10 are present in the genomes of the A. thaliana races Landsberg erecta and Columbia indicating that the superfamily comprises at least 0.1% of the A. thaliana genome. The nucleotide sequences of the reverse transcriptase regions of the ten element families place them in the category of copia-like retrotransposons and phylogenetic analysis of the amino acid sequences suggests that horizontal transfer may have played a role in their evolution.
Furandicarboxylic Acid (FDCA), A Versatile Building Block for a Very Interesting Class of Polyesters (2012)

Avantium (www.avantium.com) is developing a next generation bioplastics based on 2,5-furandicarboxylic acid (FDCA), called “YXY building blocks”, which can be produced on the basis of sugars and other, non-food, carbohydrates. Avantium aims to replace oil-based polyesters (such as PET) with Furanics polyesters (such as PEF) in a wide range of applications, including bottles and carpets. The excellent barrier properties, significant reductions in non renewable energy usage and green house gas emissions as well as the calculated cost price indicates that PEF can compete with traditional PET regarding price, performance as well as sustainability issues.
Prognostic factors in breast cancer. College of American Pathologists Consensus Statement 1999. (2000)

Factors ranked in category I included TNM staging information, histologic grade, histologic type, mitotic figure counts, and hormone receptor status. Category II factors included c-erbB-2 (Her2-neu), proliferation markers, lymphatic and vascular channel invasion, and p53. Factors in category III included DNA ploidy analysis, microvessel density, epidermal growth factor receptor, transforming growth factor-alpha, bcl-2, pS2, and cathepsin D. This report constitutes a detailed outline of the findings and recommendations of the consensus conference group, organized according to structural guidelines as defined.
Rheomolding: A New Process to Mold Polymeric Materials (1981)

Abstract Polymer processing is an engineering field which deals with the conversion of synthetic high polymers into shaped articles ready for use. Impressive developments in the field of polymer processing in the last 20 years or so were precicipitated by the need to process plastic materials with an ever-increasing rate of production within stringent quality requirements. One of the most interesting methods for shaping plastic products is molding which involved all the possible ways for stuffing molds with thermoplastic or thermosetting polymers. Injection molding, compression molding, and transfer molding have now become some of today's routine approaches to plastics shaping.
Increased expression of T‐plastin gene in cisplatin‐resistant human cancer cells: identification by mRNA differential display (1996)

The cellular resistance to the potent anticancer agent cis-diamminedichloroplatinum(II) (cisplatin) is thought to be mediated by multiple mechanisms. The technique of differential display of mRNAs was applied to various cisplatin-resistant cell lines and the corresponding parental sensitive human bladder, prostatic, and head and neck cancer cells in order to identify genes that underlie cisplatin resistance. Twenty-four clones were confirmed by Northern blot analysis to be expressed differentially between resistant and the corresponding sensitive cells. Partial DNA sequences of the eight clones that showed a threefold or greater increase in expression in either the resistant cells (seven clones) or sensitive cells (one clone) revealed that two were derived from the T-plastin gene and one from the tissue factor gene. The abundance of T-plastin mRNA in cisplatin-resistant T24/DDP10 cell was approximately 12 times that in the parental T24 cells. Transfection of T24/DDP10 cells with a vector encoding full-length T-plastin antisense RNA demonstrated that reduced T-plastin expression was associated with increased sensitivity to cisplatin. These results are consistent with the hypothesis that several mechanisms participate cooperatively in the acquisition of cisplatin resistance in human cancer.
Rearing Lygus Bugs on Artificial Diets12 (1967)

Lygus hesperus Knight and the tarnished plant bug, L. Lincolaris (Palisot de Beauvois), were successfully reared from egg to adult on artificial diets containing alfalfa meal and other nutrients. Adults laid eggs when they were fed these diet. Also, nymphs were reared and adults laid eggs on a diet without plant extracts. The bugs were fed liquid diets through Parafilm® membranes, and adults were induced to oviposit in devices constructed for that purpose. Diets and methods are described in detail. Development of all stages on artificial diets was similar to that on green beans; however, diet-fed adults had a shorter lifespan.
Ueber das Zustandekommen der Diphtherie-Immunität und der Tetanus-Immunität bei Thieren (1890)

Drug Prescribing for Patients with Chronic Kidney Disease in General Practice: a Cross-Sectional Study
Extracellular Matrix Mineralization and Osteoblast Gene Expression by Human Adipose Tissue–Derived Stromal Cells (2001)

Human adipose tissue represents an abundant reservoir of stromal cells with potential utility for tissue engineering. The current study demonstrates the ability of human adipose tissue-derived stromal cells to display some of the hallmarks of osteoblast differentiation in vitro. Following treatment with ascorbate, beta-glycerophosphate, dexamethasone, and 1,25 dihydroxy vitamin D(3), adipose tissue-derived stromal cells mineralize their extracellular matrix based on detection of calcium phosphate deposits using Alizarin Red and von Kossa histochemical stains. Fourier transform infrared analysis demonstrates the apatitic nature of these crystals. Mineralization is accompanied by increased expression or activity of the osteoblast-associated proteins osteocalcin and alkaline phosphatase. These and other osteoblast-associated gene markers are detected based on polymerase chain reaction. In contrast, the adipocyte gene markers--leptin, lipoprotein lipase, and peroxisome proliferator activated receptor gamma2--are reduced under mineralization conditions, consistent with the reciprocal relationship postulated to exist between adipocytes and osteoblasts. The current work supports the presence of a multipotent stromal cell population within human extramedullary adipose tissue. These findings have potential implications for human bone tissue bioengineering.
Tar and Nicotine in Cigarette Smoke (1969)

Abstract Cigarettes were conditioned 24 hr at 75°F and 60% relative humidity prior to being selected and smoked in a random order. Cigarettes were smoked in a Cambridge filter holder on an automatic smoking machine to a pre-determined butt length. TPM wet collection was on a CM113A filter disk and was determined as the net weight gain in the Cambridge filter holder after smoking. The filter pads were extracted with dioxane-isopropanol solution and were analyzed for moisture by gas chromatography. The pads and solvent extracts were steam distilled and the distillate was analyzed spectrophotometrically for nicotine. TPM dry was calculated as TPM wet minus nicotine and water. The coefficient of variation in this method is less than 5% with a 95% confidence level.
Phase Behavior of Asymmetrically Charged Colloids Investigated by Monte Carlo Simulation (2014)

Using Monte Carlo simulations, we investigate the structural characteristics of an interacting hard sphere system with shifted charge. The focus is placed on understanding the dependence of its phase behavior on charge asymmetry. Two different phase transitions are discovered for this model system. Upon increasing the volume fraction, a liquid-to-crystal transition first occurs at a significantly lower volume fraction in comparison to that of the centro-charged system. This is due to the stronger effective inter-particle repulsion caused by the additional charge anisotropy. Moreover, within the crystal state at higher volume fraction, the system further undergoes a disorder-to-order transition with respect to the charge orientation. The dependence of crystal formation and orientational ordering on charge asymmetry and temperature was also examined. These model studies are essential to advance our future understanding regarding the fundamental physiochemical properties of novel Janus colloidal particles and protein crystallization conditions.
Computing Marginal Distributions over Continuous Markov Networks for Statistical Relational Learning (2010)

Continuous Markov random fields are a general formalism to model joint probability distributions over events with continuous outcomes. We prove that marginal computation for constrained continuous MRFs is #P-hard in general and present a polynomial-time approximation scheme under mild assumptions on the structure of the random field. Moreover, we introduce a sampling algorithm to compute marginal distributions and develop novel techniques to increase its efficiency. Continuous MRFs are a general purpose probabilistic modeling tool and we demonstrate how they can be applied to statistical relational learning. On the problem of collective classification, we evaluate our algorithm and show that the standard deviation of marginals serves as a useful measure of confidence.
Effects of Fit and Bonding Characteristics of Femoral Stems on Adaptive Bone Remodeling (1994)

Bone atrophy caused by stress-shielding may cause serious complications for the long-term fixation of hip stems. In particular, uncemented total hip arthroplasty is threatened by this problem, because the stems are usually larger and, as a consequence, stiffer than those of cemented implants. In the present study, the effects of fit and bonding characteristics of femoral hip stems were investigated, using the (nonlinear) finite element method in combination with adaptive bone remodeling theory to predict the bone density distribution in a bone or bone/implant configuration. Unknown parameters used in the theory, such as a reference equilibrium loading stimulus and a threshold (dead) zone of this stimulus, were established (triggered) by using the method to predict the density distributions in the natural femur and around fully coated uncemented implants. The computer simulation method can provide long term predictions of remodeling patterns around various implant configurations. Several cases were analyzed, whereby the coating conditions (fully, partly, or noncoated) and the fit characteristics (press fitted or overreamed) were varied. The computer predictions showed that partly coating can only significantly reduce bone atrophy relative to fully coated stems, when the coating is applied at a small region at the utmost proximal part of the stem. For smooth press-fit stems the predicted amount of bone loss (35 percent in the proximal medial region) was less than for a one-third proximally coated or a fully coated stem (50 to 54 percent predicted bone loss in the proximal medial region).(ABSTRACT TRUNCATED AT 250 WORDS)
Predictors of greater than 80% 2-year mortality in primary myelofibrosis: a Mayo Clinic study of 884 karyotypically annotated patients (2011)

DIPSS-plus (the Dynamic International Prognostic Scoring System-plus) includes 8 risk factors for survival in primary myelofibrosis. In the present study of 884 karyotypically annotated patients with primary myelofibrosis, we sought to identify 1 or 2 parameters that can reliably predict death in the first 2 years of disease. After a median of 8.2 years from time of referral to the Mayo Clinic, 564 deaths (64% of patients in the study) had been recorded. Risk factors associated with > 80% 2-year mortality included monosomal karyotype, inv(3)/i(17q) abnormalities, or any 2 of the following: circulating blasts > 9%, leukocytes ≥ 40 × 10(9)/L, or other unfavorable karyotype. Patients with any 1 of these risk profiles (n = 52) displayed significantly shorter overall survival than those otherwise belonging to a high-risk category per DIPSS-plus (n = 298); respective median survivals were 9 and 23 months (hazard ratio 2.2, 95% confidence interval 1.6-3.1; P < .01). The present information complements DIPSS-plus in the selection of primary myelofibrosis patients for high-risk treatment approaches.
Algae: Anatomy, Biochemistry, and Biotechnology (2006)

General Overview Definition Classification Occurrence and Distribution Structure of Thallus-Cytomorphological Types Nutrition Reproduction Summaries of the 11 Algal Phyla Endosymbiosis and Origin of Eukaryotic Photosynthesis Anatomy Cytomorphology and Ultrastructure Outside the Cell Flagella and Associated Structures The photoreceptor Apparata Chloroplasts The Nucleus, Nuclear Division, and Cytokinesis Ejectile Organelles and Feeding Apparatus Photosynthesis Light Photosynthesis Light-dependent Reactions Light-Independent Reactions The Energy Relationships in Photosynthesis: The Balance Sheet Working with Light How Light Behaves Field instruments, Use and Application Radiometry Photometry Units conversion PAR Detectors The Photosynthesis-Irradiance Response Curve (P vs E Curve) Photoacclimation Biogeochemical Role of Algae The Role of Algae in Biogeochemistry Limiting Nutrients Algae and the Phosphorus Cycle Algae and the Nitrogen Cycle Algae and the Silicon Cycle Algae and the Sulfur Cycle Algae and the Oxygen-Carbon Cycles Algal Culturing Collection, Storage, and Preservation Culture Types Culture Parameters Culture Vessels Media Choice and Preparation Sterilization of Culture Materials Culture Methods Outdoor Ponds Photobioreactors Quantitative Determinations of Algal Density and Growth Algae Utilization Introduction Sources and Uses of Algae Human food Animal feed Extracts Fertilizers Cosmetics Functional Foods and Nutraceuticals Toxins Oddities and Curiosities in the Algal World In the Realm of Darkness Algae-Animal Interaction: Riding a Sloth, Swinging on a Spider Web, Swimming in a Jelly Someone Like it Cold Someone Like it Hot Someone Like it Dry