gebhart/patrepeval huggingface dataset:
OPTIMISTIC ABOUT ANTISENSE (1997)

RETURN TO ISSUEPREVNewsNEXTOPTIMISTIC ABOUT ANTISENSEPromising clinical results and chemical strategies for further improvements delight antisense drug researchersREBECCA L. RAWLSView Author Information C&EN WashingtonCite this: Chem. Eng. News 1997, 75, 22, 35–39Publication Date (Print):June 2, 1997Publication History Published online12 November 2010Published inissue 2 June 1997https://pubs.acs.org/doi/10.1021/cen-v075n022.p035https://doi.org/10.1021/cen-v075n022.p035newsACS PublicationsCopyright © 1997 American Chemical SocietyArticle Views10Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Biotechnology Get e-Alerts
Cancer Stem Cells and Radiotherapy: New Insights Into Tumor Radioresistance (2006)

EDITORIALS 1755 Solid tumors are histologically heterogeneous and include tumor cells, stroma, infl ammatory infi ltrates, and vascular structures. Furthermore, it has long been recognized that subpopulations of cancer cells exist within the tumors that resemble the developmental hierarchy of the normal tissue from which the tumor arose . In recent years, the cancer stem cell model of tumorigenesis has received increasing attention. This model posits that tumors are driven and maintained by a minority subpopulation of cells that have the capacity to self-renew (i.e., give rise to progeny with similar properties as themselves) and to generate the more differentiated progeny which make up the bulk of a tumor (1) . The former population has been termed cancer stem cells (CSCs), tumorigenic cancer cells, or tumor-initiating cells, by various investigators, to indicate that only they can give rise to new tumors when transplanted into immunodefi cient animals. Evidence for the existence of CSCs initially came from studies of acute myelogenous leukemia (AML), among which a subset of leukemic cells was identifi ed that gave rise to AML in immunodefi cient mice and that displayed a similar cell surface immunophenotype as normal hematopoietic stem cells (2) . Subsequently,
Deoxyribonucleic Acid Relatedness between Serogroups and Serovars in the Family Leptospiraceae with Proposals for Seven New Leptospira Species (1987)

Deoxyribonucleic acid hybridization (hydroxyapatite method, 55 and 70°C) was used to characterize 38 serovars from 22 named serogroups of Leptospira interrogans and Leptospira biflexa, 6 serovars from 4 new unnamed serogroups of Leptospira interrogans, and single serovars of the proposed species Leptospira parva and Leptonema illini. Deoxyribonucleic acid relatedness confirmed the validity of Leptospira parva and Leptonema illini. The well-accepted species Leptospira interrogans and Leptospira biflexa, as currently defined, were extremely heterogeneous. Relatedness results revealed at least five new species among the parasitic serovars formerly included in Leptospira interrogans and two new species among the saprophytic serovars formerly included in Leptospira biflexa. Serogrouping did not equate with species identification, as serovars from several different subserogroups belonged to different species. The new species named in this paper are Leptospira, noguchii, Leptospira weilii, Leptospira santarosai, Leptospira borgpetersenii, Leptospira meyeri, Leptospira wolbachii, and Leptospira inadai.
워커 퍼시의 『영화구경꾼』에 나타난 포스트모던 세계에서의 자아 인식 (2002)

Walker Percy`s novels, essays, and interviews express the view of a writer interested in developing an integral vision of what it means to live as a human being in the disordered twentieth century. In Moviegoer Percy portrays Binx`s search for his authentic self which is lost in everydayness of a modern society. Binx finds himself wandering in the malaise, assailed by its deadly forms of inauthenticity and lost in everydayness. Then Binx initiates his search for authentic self. In his search Binx needs to perceive reality unveiled from everydayness in order to get his authentic being. Binx experiences momentary release from the grip of everydayness by exercising a rotation. This rotation along with repetition makes it possible for Binx escaper from his everyday condition. But what Binx realizes after all is ultimate despair of human condition as well as his own predicament. Binx`s sense of despair indicates his jailure to deliver himself from the predicament of lost-self in the world. But ironically this failure leads him to understand the nature of self in the post-modern world. In other words, Binx in his despair comes to understand that his self is not formulable of its meaning in the same way that a sign cannot have any meaning by itself, and he realizes that the meaning of self is constantly changing and developing depending on its relation to others. This comprehension of the nature of self then provides Binx with a sense of quiet assurance, the assurance of a man at home with the world and with himself. Accordingly, this paper will try to show how Binx perceives his lost self in the world and how he tries to recover his authentic self in his search. Then, finally, this paper will explore the meaning of Binx`s failure in his search for authentic self in order to manifest that his failure contributes to Binx`s understanding of the nature of self in the post-modern world.
Anticonvulsants in neuropathic pain: rationale and clinical evidence (2002)

Neuropathic pain, whether of peripheral or central origin, is characterized by a neuronal hyperexcitability in damaged areas of the nervous system. In peripheral neuropathic pain, damaged nerve endings exhibit abnormal spontaneous and increased evoked activity, partly due to an increased and novel expression of sodium channels. In central pain, although not explored in detail, the spontaneous pain and evoked allodynia are also best explained by a neuronal hyperexcitability. The peripheral hyperexcitability is due to a series of molecular changes at the level of the peripheral nociceptor, in dorsal root ganglia, in the dorsal horn of the spinal cord, and in the brain. These changes include abnormal expression of sodium channels, increased activity at glutamate receptor sites, changes in γ‐aminobutyric acid (GABA‐ergic) inhibition, and an alteration of calcium influx into cells. The neuronal hyperexcitability and corresponding molecular changes in neuropathic pain have many features in common with the cellular changes in certain forms of epilepsy. This has led to the use of anticonvulsant drugs for the treatment of neuropathic pain. Carbamazepine and phenytoin were the first anticonvulsants to be used in controlled clinical trials. Studies have shown these agents to relieve painful diabetic neuropathy and paroxysmal attacks in trigeminal neuralgia. Subsequent studies have shown the anticonvulsant gabapentin to be effective in painful diabetic neuropathy, mixed neuropathies, and postherpetic neuralgia. Lamotrigine, a new anticonvulsant, is effective in trigeminal neuralgia, painful peripheral neuropathy, and post‐stroke pain. Other anticonvulsants, both new and old, are currently undergoing controlled clinical testing. The most common adverse effects of anticonvulsants are sedation and cerebellar symptoms (nystagmus, tremor and incoordination). Less common side‐effects include haematological changes and cardiac arrhythmia with phenytoin and carbamazepine. The introduction of a mechanism‐based classification of neuropathic pain, together with new anticonvulsants with a more specific pharmacological action, may lead to more rational treatment for the individual patient with neuropathic pain. © 2002 European Federation of Chapters of the International Association for the Study of Pain
Blockage of autophagy pathway enhances Salmonella tumor-targeting (2016)

Previous studies have shown that strains of Salmonella typhimurium specifically target tumors in mouse models of cancer. In this study, we report that tumor-targeting Salmonella typhimurium A1-R (A1-R) or VNP20009 induced autophagy in human cancer cells, which serves as a defense response. Functionally, by knockdown of essential autophagy genes Atg5 or Beclin1 in bacteria-infected cancer cells, the autophagy pathway was blocked, which led to a significant increase of intracellular bacteria multiplication in cancer cells. Genetic inactivation of the autophagy pathway enhanced A1-R or VNP20009-mediated cancer cell killing by increasing apoptotic activity. We also demonstrate that the combination of pharmacological autophagy inhibitors chloroquine (CQ) or bafilomycin A1 (Baf A1) with tumor-targeting A1-R or VNP20009 significantly enhanced cancer-cell killing compared with Salmonella infection alone. These findings provide a proof-of-concept of combining autophagy inhibitors and tumor-targeting Salmonella to enhance cancer-cell killing.
Regulation of development in Myxococcus xanthus: effect of 3':5'-cyclic AMP, ADP, and nutrition. (1975)

An assay was developed to study the regulation of fruiting in Myxococcus xanthus. The nucleotides, adenosine 3':5'-cyclic monophosphate (cyclic AMP) and adenosine diphosphate (ADP), were found to greatly stimulate fruiting under the assay conditions. Very sharp concentration optima were observed. Even under conditions of starvation, these nucleotides greatly increased the number of aggregation sites. Nutrition was found to influence fruiting body morphology. The effect of amino acids on the nucleotide stimulation of fruiting was studied under our assay conditions. L-Methionine and L-isoleucine (1 mM) completely blocked either L-threonine or D,L-diaminopimelic acid synergistically enhanced the amount of fruiting in the presence of these nucleotides. The data presented suggest the existence of differentiation-related regulatory compounds in M. xanthus.
Enhanced fluorescence transduction properties of metallic nanocavity arrays (2005)

We studied fluorescence enhancement of molecular species bound within metallic nanocavities. These nanostructures possess a number of desirable properties for real-time microarrays, such as localization of excitation light within the nanocavities, strong isolation from fluorescence produced by unbound species, and an apparent increase in fluorescence yield for bound species. Experimental measurements show nearly a factor of two increase in excitation intensity within the nanocavities, and factor of six increase in yield. A simple electromagnetic model of a dipole within a nanocavity shows an increase in radiative output consistent with our yield estimates, and also verifies the strong fluorescence isolation from species lying outside the nanocavity.
The Genome of <i>Dictyostelium discoideum</i> (2006)

The Dictyostelium discoideum genome has been sequenced, assembled and annotated to a high degree of reliability. The parts-list of proteins and RNA encoded by the six chromosomes can now be accessed and analyzed. One of the initial surprises was the remarkably large number of genes that are shared with plants, animals, and fungi that must have been present in their common progenitor over a billion years ago. The genome encodes a total of about 10,300 proteins including protein families involved in cytoskeletal control, posttranslational protein modification, detoxification, secondary metabolism, cell adhesion, and signal transduction. The genome has a higher proportion of homopolymeric tracts and simple sequence repeats, such as [CAA]n, than most other genomes. Triplet repeats in translated regions produce the highest known proportion of polyglutamine tracts in any known proteome. Phylogenetic analyses based on complete proteomes confirm that the amoebozoa are a sister group to the animals and fungi, distinct from plants and early diverging species such as Leishmania, Plasmodium, or Giardia. The completed Dictyostelium sequence opens the door to large-scale functional exploration of its genome.
Non‐degradative dissolution and acetylation of ball‐milled plant cell walls: high‐resolution solution‐state NMR (2003)

Two solvent systems for fully dissolving, and optionally derivatizing, finely ground plant cell wall material at room temperature are described: dimethylsulfoxide (DMSO) and tetrabutylammonium fluoride (TBAF) or N-methylimidazole (NMI). In situ acetylation produces acetylated cell walls (Ac-CWs) that are fully soluble in chloroform. Lignin structures tested remain fully intact. The dispersion of 13C-1H correlations afforded by two-dimensional (2D) nuclear magnetic resonance (NMR) experiments reveals the major lignin units, allowing the whole lignin fraction to be analyzed by high-resolution solution-state NMR methods for the first time. Non-degradative cell wall dissolution offers the potential to analyze polysaccharide components, and improve current cell wall analytical methods by using standard homogeneous solution-state chemistry.
Alterations of the basement membrane and connective tissue antigens in human metastatic lymph nodes (1983)

Antigens of the basement membrane (type-IV collagen and laminin) and the connective tissue (type-III collagen and fibronectin) were studied by immunofluorescence in 16 lymph nodes draining colorectal carcinomas and 6 lymph nodes draining breast carcinomas. A comparison was also made between 7 primary colorectal carcinomas and 9 lymph nodes draining these tumors. Anti-type-IV collagen and anti-laminin rarely stained the basement membrane of metastatic tumors. In contrast, we detected type-IV collagen in the peritumoral stroma, although similar images were rarely seen in primary tumors. When tumoral cells were in the vicinity of lymphoid cells, they were occasionally separated by a barrier stained by the four antisera, or only by antifibronectin and anti-type-III collagen. In other cases no barrier was observed between both types of cells which were in close contact. On the whole the above alterations were more marked in the lymph nodes draining breast carcinomas, in comparison to those draining colorectal carcinomas. Tumor cells were never stained by anti-type-IV collagen or antilaminin serum. Some cells found either in the lymphoid or in the tumor area of metastatic lymph nodes were stained not only by these antisera, but also by a monoclonal antibody against Willebrand Factor, which is a marker of endothelial cells. Thus the labelled cells were characterized as being derived from the capillary wall.
Overactive detrusor (1999)

Detrusor overactivity poses a major challenge to physicians from many specialities. Progress in our understanding of the pathogenesis of detrusor overactivity is slow but steady. Advances in treatment continue to be made, both with new agents and new methods of delivering older drugs. Neuromodulation is showing great promise as experience increases, and developments in bladder augmentation offer hope for lower morbidity treatments in future.
High-Q VHF micromechanical contour-mode disk resonators (2002)

A micromechanical, laterally vibrating disk resonator, fabricated via a technology that combines polysilicon surface-micromachining and metal electroplating to attain submicron lateral capacitive gaps, has been demonstrated at frequencies approaching 160 MHz with Q's as high as 9,400-the highest demonstrated to date for an on-chip resonator in this frequency range. This frequency also represents the highest to date for an electrostatically transduced micromechanical resonator and is an important step towards reaching the frequencies required by the RF front-ends in wireless transceivers. The geometric dimensions necessary to reach a given frequency are larger for this contour-mode than for the flexural-modes used by previous resonators. This, coupled with its unprecedented Q value, makes this disk resonator a choice candidate for use in the IF and RF stages of future miniaturized transceivers.
Origin and Distribution of Capsaicin-Sensitive Substance P-Immunoreactive Nerves in the Nasal Mucosa (1983)

In immunohistochemical studies, substance P-immunoreactivity (SP-IR) was found in a population of trigeminal ganglion cells in guinea pig, rat and cat. SP-IR nerve endings were found in the spinal trigeminal nucleus, around sphenopalatine ganglion cells, around blood vessels, as well as under and within the epithelium of the nasal mucosa. Ligation and denervation experiments in the cat indicated that the SP-IR nerves in the sphenopalatine ganglion and the nasal mucosa are of trigeminal origin. Capsaicin pretreatment of guinea pigs and rats resulted in a selective loss of the SP-IR nerves in the nasal mucosa and sphenopalatine ganglion, while the parasympathetic and sympathetic nerves were still present.
Characterization of murine monoclonal antibodies that recognize neutralizing epitopes on human C5a (1987)

We generated a panel of 10 murine monoclonal antibodies (MAbs) that recognize human complement fragment C5a. These MAbs were characterized for their ability to immunoprecipitate 125I-labeled C5a, bind C5a in solid-phase enzyme immunoassay, and block 125I-labeled C5a binding to polymorphonuclear leukocytes. Four of these MAbs had affinity constants for C5a in the 1 X 10(9) to 3 X 10(9) M-1 range. These MAbs blocked C5a-induced neutrophil polarization and chemiluminescence. They blocked the ability of passively administered C5a to cause neutropenia in rabbits. These anti-C5a neutralizing MAbs may have potential therapeutic use in states of complement activation.
Diverse macrophage populations mediate acute lung inflammation and resolution (2014)

Acute respiratory distress syndrome (ARDS) is a devastating disease with distinct pathological stages. Fundamental to ARDS is the acute onset of lung inflammation as a part of the body's immune response to a variety of local and systemic stimuli. In patients surviving the inflammatory and subsequent fibroproliferative stages, transition from injury to resolution and recovery is an active process dependent on a series of highly coordinated events regulated by the immune system. Experimental animal models of acute lung injury (ALI) reproduce key components of the injury and resolution phases of human ARDS and provide a methodology to explore mechanisms and potential new therapies. Macrophages are essential to innate immunity and host defense, playing a featured role in the lung and alveolar space. Key aspects of their biological response, including differentiation, phenotype, function, and cellular interactions, are determined in large part by the presence, severity, and chronicity of local inflammation. Studies support the importance of macrophages to initiate and maintain the inflammatory response, as well as a determinant of resolution of lung inflammation and repair. We will discuss distinct roles for lung macrophages during early inflammatory and late resolution phases of ARDS using experimental animal models. In addition, each section will highlight human studies that relate to the diverse role of macrophages in initiation and resolution of ALI and ARDS.
Multiple-wavelength vertical-cavity surface-emitting laser arrays with a record wavelength span (1996)

Multiple-wavelength vertical-cavity surface-emitting laser (VCSEL) arrays are fabricated using a modified patterned-substrate growth technique in a molecular beam epitaxy (MBE) system. We achieved a large lasing wavelength span of 62.7 nm and highly uniform threshold currents with an average of 2.16/spl plusmn/0.81 mA. High repeatibility of wavelength spacing between 15 arrays with sharp wavelength shift rate 117.14 nm/mm is achieved.
The Novel Binding Mode of N-Alkyl-N‘-hydroxyguanidine to Neuronal Nitric Oxide Synthase Provides Mechanistic Insights into NO Biosynthesis (2002)

A series of N-alkyl-N'-hydroxyguanidine compounds have recently been characterized as non-amino acid substrates for all three nitric oxide synthase (NOS) isoforms which mimic NO formation from N(omega)-hydroxy-L-arginine. Crystal structures of the nNOS heme domain complexed with either N-isopropyl-N'-hydroxyguanidine or N-butyl-N'-hydroxyguanidine reveal two different binding modes in the substrate binding pocket. The binding mode of the latter is consistent with that observed for the substrate N(omega)-hydroxy-L-arginine bound in the nNOS active site. However, the former binds to nNOS in an unexpected fashion, thus providing new insights into the mechanism on how the hydroxyguanidine moiety leads to NO formation. Structural features of substrate binding support the view that the OH-substituted guanidine nitrogen, instead of the hydroxyl oxygen, is the source of hydrogen supplied to the active ferric-superoxy species for the second step of the NOS catalytic reaction.
Structure and antigenicity of the phosphorylated lipopolysaccharide antigens from the leprosy and tubercle bacilli. (1986)

A family of major arabinose- and mannose-containing phosphorylated lipopolysaccharides was isolated from Mycobacterium leprae and Mycobacterium tuberculosis. The only antigenic member of the family, lipoarabinomannan (LAM)-B, was purified by anion exchange and gel filtration chromatography in detergent and recovered in large quantities (15 mg/g of bacteria). It yielded a broad diffuse band on polyacrylamide gel electrophoresis but appeared homogeneous by this criterion and gel filtration. Besides arabinose and mannose, it contained glycerol and a polyol phosphate and was acylated by lactate, succinate, palmitate, and 10-methyloctadecanoate. The phosphate was released by alkalinolysis and identified by thin layer chromatography and gas chromatography-mass spectrometry as myoinositol 1-phosphate. Thus, the group-specific "arabinomannan" of the genus Mycobacterium in the native state is acylated, contains the substituents of phosphatidylinositol, and is apparently membrane associated. LAM-B is one of the dominant immunogens of the leprosy bacillus reacting readily with antibodies from lepromatous leprosy patients and monoclonal antibodies in plate and nitrocellulose enzyme-linked immunosorbent assay and on electrophoretic immunoblots. It is immunologically cross-reactive with a like product from M. tuberculosis. LAM-B is clearly the pervasive "glycoprotein" antigen of the leprosy bacillus and may be the long sought lipoteichoic acid-like polymer of Mycobacterium with a role in cell wall physiology, macrophage recognition, and perhaps an involvement in cross-protective immunity.