gebhart/patrepeval huggingface dataset:
Isolation of Lactobacillus salivarius 1077 (NRRL B-50053) and Characterization of Its Bacteriocin, Including the Antimicrobial Activity Spectrum (2011)

Lactobacillus salivarius 1077 (NRRL B-50053) was isolated from poultry intestinal materials, and in vitro anti-Campylobacter jejuni activity was demonstrated. The isolate was then used for bacteriocin production and its enrichment. The protein content of the cell-free supernatant from the spent medium was precipitated by ammonium sulfate and dialyzed to produce the crude antimicrobial preparation. A typical bacteriocin-like response of sensitivity to proteolytic enzymes and resistance to lysozyme, lipase, and 100°C was observed with this preparation. The polypeptide was further purified by gel filtration, ion-exchange, and hydrophobic-interaction chromatography. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), Edman degradation, and isoelectrofocusing were used to characterize its 3,454-Da molecular mass, the amino acid sequence of its 37 residue components, and the isoelectric point of pI 9.1 of the bacteriocin. Bacteriocin L-1077 contained the class IIa bacteriocin signature N-terminal sequence YGNGV. MICs of bacteriocin L-1077 against 33 bacterial isolates (both Gram negative and Gram positive) ranged from 0.09 to 1.5 μg/ml. Subsequently, the therapeutic benefit of bacteriocin L-1077 was demonstrated in market-age (40- to 43-day-old) broiler chickens colonized with both C. jejuni and Salmonella enterica serovar Enteritidis. Compared with untreated control birds, both C. jejuni and S. Enteritidis counts in colonized ceca were diminished by >4 log(10) and S. Enteritidis counts in both the liver and the spleen of treated birds were reduced by 6 to 8 log(10)/g compared with those in the nontreated control birds. Bacteriocin L-1077 appears to hold promise in controlling C. jejuni/S. Enteritidis among commercial broiler chickens.
A New Fecal Calprotectin Test for Colorectal Neoplasia: Clinical Results and Comparison with Previous Method (2001)

The new simple method, using small samples of feces, had a higher diagnostic accuracy, suggesting that it should be preferred to the original one, in screening high risk groups for CRC.
On the Mechanisms of Haemodialysis-induced Neutropenia: A Study with Five New and Re-used Membranes (1988)

A prospective study was undertaken in 12 haemodialysed patients successively treated on five new as well as re-used dialyser membranes, that is cuprophane, cellulose acetate, polysulphone, polycarbonate, and polyacrylonitrile. A significant reduction of neutrophils occurred with every membrane during their first use, which improved only with cuprophane upon re-use. Thrombocytopenia was noted only when neutropenia reached very low values. Monocyte reduction occurred on cuprophane, cellulose acetate and polycarbonate, but did not improve during second use. C3d accumulation paralleled the time course of neutropenia only with cuprophane and cellulose acetate. Plasma collected at the extreme of neutropenia induced aggregation of control and predialysis cells, but did not aggregate autologous dialysed neutrophils collected at 5 min. Our data indicate that the mechanism linking complement activation to neutropenia is probably triggered by more than one factor.
Mechanisms of cellular penetration and nuclear localization of an anti-double strand DNA autoantibody (1996)

An anti-dsDNA Ab, mAb 3E10, was identified that bound membranes of fixed human renal tubular cells, penetrated live murine renal tubular cells in vivo, and localized in the cell nucleus. mAb 3E10 binds both dsDNA and an extracellular matrix protein, HP8/HEVIN, expressed in high endothelial venules. Previous studies showed both shared and distinct binding determinants of mAb 3E10 VH for DNA and HP8/HEVIN. To independently assess the requirement of DNA and HP8/HEVIN in cellular penetration, site-directed mutants of mAb 3E10 VH and V kappa were studied for penetrating kidney cell lines. The results showed that residues required for binding DNA, but not HP8/HEVIN, were necessary for Ab penetration, indicating that cellular penetration required the presence of DNA or binding of Ab to a membrane determinant precisely resembling DNA. Ab Fab penetrated cells, indicating that neither the Fc nor multivalent Ab binding is necessary for cellular penetration. Ab synthesized in the cytoplasm as a result of deleting heavy and light chain signal peptides was not translocated to the nucleus, indicating a mechanism distinct from the usual protein nuclear localization signals and suggesting the need for a membrane-mediated pathway or for post-translational modification of the Ab.
Requirement of Fnr and NarL functions for nitrate reductase expression in Escherichia coli K-12 (1982)

I used a chlC-lac operon fusion to study regulatory mutations which affect nitrate reductase expression in Escherichia coli. A NarL- mutant apparently lacks a nitrate-specific positive regulatory component. Furthermore, an fnr (nirR) mutation prevented enzyme induction under any conditions. These data are consistent with a two-step, positive control model for nitrate reductase regulation.
Liposomal Formulations in Clinical Use: An Updated Review (2017)

Liposomes are the first nano drug delivery systems that have been successfully translated into real-time clinical applications. These closed bilayer phospholipid vesicles have witnessed many technical advances in recent years since their first development in 1965. Delivery of therapeutics by liposomes alters their biodistribution profile, which further enhances the therapeutic index of various drugs. Extensive research is being carried out using these nano drug delivery systems in diverse areas including the delivery of anti-cancer, anti-fungal, anti-inflammatory drugs and therapeutic genes. The significant contribution of liposomes as drug delivery systems in the healthcare sector is known by many clinical products, e.g., Doxil<sup>®</sup>, Ambisome<sup>®</sup>, DepoDur™, etc. This review provides a detailed update on liposomal technologies e.g., DepoFoam™ Technology, Stealth technology, etc., the formulation aspects of clinically used products and ongoing clinical trials on liposomes.
Applications of site‐specific chemical modification in the manufacture of biopharmaceuticals: I. An overview (1997)

The chemical modification of proteins has long been a useful approach to elucidating structure-function relationships. Recently, these same chemistries have been finding application in the preparation of proteins for both diagnostic and therapeutic use. These applications include alterations to introduce new properties, such as improved stability, or new traits, such as drug binding and transport, that take advantage of both broad and narrow ranges of selectivity. Site-specific chemical modification of proteins, when combined with the powerful advantages of site-specific mutagenesis, can yield protein agents superior to those generated by either approach alone.
An Experimental Study of the Relationship Between Color Blindness and Reading Achievement in the First Grade (1963)

The purpose of this study was to investigate and analyze the reading achievement of color blind children at the end of the first-grade in five randomly selected Albuquerque Public Elementary Schools to determine if there are any significant differences in their reading achievement and that of color vision children with similar I.Q. scores and to find if there is a relationship between this defect of vision and reading achievement.
Osteopenia in Adolescent Idiopathic Scoliosis (2001)

Bone biopsy study suggested disturbance of bone turnover in patients with adolescent idiopathic scoliosis. The abnormal metabolism might contribute to the low bone mineral density and play an important role in the etiology and pathogenesis of adolescent idiopathic scoliosis.
Chemical biology of tetracycline antibioticsThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Systems and Chemical Biology, and has undergone the Journal's usual peer review process. (2008)

For more than half a century, tetracycline antibiotics have been used to treat infectious disease. However, what once used to be a commonly prescribed family of antibiotics has now decreased in effectiveness due to wide-spread bacterial resistance. The chemical scaffold of the tetracyclines is a versatile and modifiable structure that is able to interact with many cellular targets. The recent availability of detailed molecular interactions between tetracycline and its cellular targets, along with an understanding of the tetracycline biosynthetic pathway, has provided us with a unique opportunity to usher in a new era of rational drug design. Herein we discuss recent findings that have clarified the mode of action and the biosynthetic pathway of tetracyclines and that have shed light on the chemical biology of tetracycline antibiotics.
A recombinant amino terminal fragment of bactericidal/permeability-increasing protein inhibits the induction of leukocyte responses by LPS (1993)

Bactericidal/permeability-increasing protein (BPI) is a major component of the granules of polymorphonuclear neutrophils (PMNs) and is involved in the killing of gram-negative bacteria. A 23-kd recombinant protein, corresponding to the NH2-terminal fragment of human BPI (rBPI23), has been shown to bind lipid A and antagonize some lipopolysaccharide (LPS)-mediated effects. In this study the ability of rBPI23 to prevent a wide range of cellular responses to LPS was investigated. In vitro assays were carried out using human blood to more closely approximate in vivo conditions. The release of proinflammatory cytokines [tumor necrosis factor (TNF), interleukin-1 beta (IL-1 beta), IL-6, IL-8], induced by E. coli O113 LPS, was markedly reduced by rBPI23 in a concentration-dependent fashion. The production of the anti-inflammatory protein IL-1ra (IL-1 receptor antagonist) was triggered by lower LPS concentrations than those necessary for the other cytokines. Furthermore, prevention of IL-1ra release required higher rBPI23 concentrations than for other cytokines. The LPS-induced production of oxygen-derived free radicals by phagocytic cells (resulting in chemiluminescence) was also prevented by rBPI23. The inhibition was specific for LPS because the activation of leukocytes by phorbol myristate acetate, zymosan, or TNF was unaffected by BPI. The ability of rBPI23 to antagonize specifically the effects of endotoxin in the complex environment of human blood along with its bactericidal activity suggests that rBPI23 may be a novel therapeutic agent in the treatment of gram-negative infections.
Cloud-based synchronization of distributed file system hierarchies (2010)

As the number of user-managed devices continues to increase, the need for synchronizing multiple file hierarchies distributed over devices with ad hoc connectivity, is becoming a significant problem. In this paper, we propose a new approach for efficient cloud-based synchronization of an arbitrary number of distributed file system hierarchies. Our approach maintains both the advantages of peer-to-peer synchronization with the cloud-based approach that stores a master replica online. In contrast, we do not assume storage of any user's data in the cloud, so we address the related capacity, cost, security, and privacy limitations. Finally, the proposed system performs data synchronization in a peer-to-peer manner, eliminating cost and bandwidth concerns that arise in the “cloud master-replica” approach.
An Apolipoprotein Homolog of Rat Apolipoprotein A‐IV in Human Plasma (1979)

A protein with properties similar to apoprotein A‐IV of Rat high‐density lipoproteins has been isolated from the lipoprotein fraction (δ &lt; 1.006 g/ml) of individual non‐fasting hypertriglyceridaemic subjects. The M r of human apolipoprotein A‐IV determined by polyacrylamide‐gel electrophoresis in sodium dodecylsulfate and by column chromatography in 6 M guanidine hydrochloride is 46000. The amino acid composition is significantly different from any previously characterised human apolipoprotein but resembles that of rat apolipoprotein A‐IV. The N‐terminal amino acid of human and rat apolipoprotein A‐IV is glutamate or glutamine. The p I of the major human A‐IV band in 6 M urea is ∼ 5.15. Immunochemically apolipoprotein A‐IV is not identical with any of the well known human apolipoproteins. Human apolipoprotein A‐IV is present in all fractions of δ &lt; 1.006 g/ml isolated by a single ultracentrifugal spin from non‐fasting subjects, but is lost from lipoproteins of δ &lt; 1.006 g/ml upon recentrifugation. Immunoelectrophoretic studies show that most of human apolipoprotein A‐IV is present in the fraction of δ &gt; 1.21 g/ml and is unassociated with the major lipoprotein fractions in serum. Charge‐shift electrophoresis however clearly demonstrates the hydrophobic character of human apolipoprotein A‐IV. There is evidence that apolipoprotein A‐IV is a constituent of chylomicrons that is removed from the particle surface when chylomicrons enter the plasma compartment.
Real-Time Imaging of Single-Molecule Fluorescence with a Zero-Mode Waveguide for the Analysis of Protein−Protein Interaction (2008)

Real-time imaging of single-molecule fluorescence with a zero-mode waveguide (ZMW) was achieved. With modification of the ZMW geometry, the signal-to-background ratio is twice that obtainable with a conventional ZMW. The improved signal-to-background ratio makes it possible to visualize individual binding-release events between chaperonin GroEL and cochaperonin GroES at a concentration of 5 microM. Two rate constants representing two-timer kinetics in the release of GroES from GroEL were measured with the ZMW, and the measurements agreed well with those made with a total internal reflection fluorescence microscopy. These results indicate that the novel ZMW makes feasible the direct observation of protein-protein interaction at an intracellular concentration in real time.
A Factor XIIa Inhibitory Antibody Provides Thromboprotection in Extracorporeal Circulation Without Increasing Bleeding Risk (2014)

Currently used anticoagulants prevent thrombosis but increase bleeding. We show an anticoagulation therapy without bleeding risk based on a plasma protease factor XII function-neutralizing antibody. We screened for antibodies against activated factor XII (FXIIa) using phage display and demonstrated that recombinant fully human antibody 3F7 binds into the FXIIa enzymatic pocket. 3F7 interfered with FXIIa-mediated coagulation, abolished thrombus formation under flow, and blocked experimental thrombosis in mice and rabbits. We adapted an extracorporeal membrane oxygenation (ECMO) cardiopulmonary bypass system used for infant therapy to analyze clinical applicability of 3F7 in rabbits. 3F7 provided thromboprotection as efficiently as heparin, and both drugs prevented fibrin deposition and thrombosis within the extracorporeal circuit. Unlike heparin, 3F7 treatment did not impair the hemostatic capacity and did not increase bleeding from wounds. These data establish that targeting of FXIIa is a safe mode of thromboprotection in bypass systems, and provide a clinically relevant anticoagulation strategy that is not complicated by excess bleeding.
Role of LFA‐1, ICAM‐1, VLA‐4 and VCAM‐1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro (1996)

The blood-retinal barrier (BRB), which is composed of the retinal pigment epithelium (RPE) and retinal vascular endothelium, normally restricts the traffic of lymphocytes into the retina. During ocular inflammatory conditions such as posterior uveitis there is a large increase in lymphocyte migration across the BRB. The differential role played by the two barrier sites, however, remains unclear. To evaluate the role of the posterior BRB, the migration of CD4+ antigen-specific T-cell line through rat RPE cell monolayers was investigated in vitro using time-lapse videomicroscopy. The adhesion molecules involved in controlling transepithelial migration across normal and interferon-gamma (IFN-gamma)-activated RPE was assessed with monoclonal antibodies directed against cell adhesion molecules. Lymphocytes were treated with antibodies specific for CD11a (alpha L subunit of LFA-1), CD18 (beta 2 subnit of the leucam family) and CD49 d (alpha 4 subnit of very late activation antigen-4, VLA-4), and the RPE with antibodies specific for CD54 (intracellular adhesion molecule-1, ICAM-1) and CD 106 (vascular cell adhesion molecule-1, VCAM-1). Migration across unstimulated RPE was inhibited by antibodies to ICAM-1 (48.6 +/- 3.5% reduction), leucocyte functional antigen-1 (LFA-1) alpha (61 +/- 5.2%) and LFA-1 beta (63.2 +/- 4.7%), but not by antibodies to VLA-4. VCAM-1 was not expressed on untreated RPE. Following activation of the RPE monolayers for 72 hr with IFN-gamma, antibodies to LFA-1 alpha, LFA-1 beta and ICAM-1 inhibited migration by 49.9 +/- 9.4%, 63.6 +/- 5.5% and 47.7 +/- 4.2% respectively. Antibodies to VLA-4 and VCAM-1 blocked migration by 21.5 +/- 8.4% and 32.3 +/- 6.2%, respectively, which correlated with the induction of VCAM-1 expression on RPE and increased migration. Under these conditions blocking both VCAM-1 and ICAM-1 reduced migration by 70.9 +/- 2.3%, which was greater than the effect of blocking either of these molecules alone. These results demonstrate that the posterior barrier of the BRB utilizes the same principle receptor-ligand pairings in controlling lymphocyte traffic into the retina as the vascular endothelium of the anterior BRB.
Pharmacodynamic monitoring of immunosuppressive drugs. (1996)

Pharmacodynamic (PD) monitoring measures the biological response to a drug, which alone—or coupled with pharmacokinetics— provides a novel method for optimization of drug dosing. PD monitoring has been investigated by us and other investigators primarily for four immunosuppressive drugs: cyclosporine (CsA), azathioprine (AZA), mycophenolate mofetil (MMF), and rapamycin (RAPA). PD monitoring of CsA and MMF involves measuring the activity of the enzymes calcineurin and inosine monophosphate dehydrogenase, respectively. The PD of AZA is assessed by measuring the activity of thiopurine methyltransferase, which is induced by a metabolite of AZA, 6-mercaptopurine. The PD for RAPA involves measuring the activity of a P70 S6 kinase in lymphocytes. To date, the most detailed studies have been performed with PD monitoring of CsA and MMF. Similarities exist in the PD responses to CsA and MMF in renal-transplant patients. At trough concentrations in blood, both drugs reduce the activity of their target enzymes by only 50%; however, considerable interpatient variability is evident. Throughout the dosing interval, the enzyme activities parallel the respective drug concentrations. AZA treatment of renaltransplant patients who exhibited an increase in thiopurine methyltransferase activity from time of transplantation resulted in fewer episodes of active rejection. Additional clinical trials are currently underway to relate various pharmacokinetics and PD parameters to clinical response, to ascertain which provides the best guide for dosing. PD monitoring may provide an alternative approach to additional measurements of drug concentrations.
PERCEPTION-BASED SIMPLIFICATION FOR BINAURAL ROOM AURALISATION (2006)

Presented at the 12th International Conference on Auditory Display (ICAD), London, UK, June 20-23, 2006.
Feature-Based Approach for the Registration of Pushbroom Imagery with Existing Orthophotos (2017)

Low-cost Unmanned Airborne Vehicles (UAVs) are rapidly becoming suitable platforms for acquiring remote sensing data for a wide range of applications. For example, a UAV-based mobile mapping system (MMS) is emerging as a novel phenotyping tool that delivers several advantages to alleviate the drawbacks of conventional manual plant trait measurements. Moreover, UAVs equipped with direct geo-referenced frame cameras and pushbroom scanners can acquire geospatial data for comprehensive high-throughput phenotyping. UAVs for mobile mapping platforms are low-cost and easy to use, can fly closer to the objects, and are filling an important gap between ground wheel-based and traditional manned-airborne platforms. However, consumer-grade UAVs are capable of carrying only equipment with a relatively light payload and their flying time is determined by a limited battery life. These restrictions of UAVs unfortunately force potential users to adopt lower-quality direct geo-referencing and imaging systems that may negatively impact the quality of the deliverables. Recent advances in sensor calibration and automated triangulation have made it feasible to obtain accurate mapping using low-cost camera systems equipped with consumer-grade GNSS/INS units. However, ortho-rectification of the data from a linear-array scanner is challenging for low-cost UAV systems, because the derived geo-location information from pushbroom sensors is quite sensitive to the performance of the implemented direct geo-referencing unit. This thesis presents a novel approach for improving the ortho-rectification of hyperspectral pushbroom scanner imagery with the aid of orthophotos generated from frame cameras through the identification of conjugate features while modeling the impact of residual artifacts in the direct geo-referencing information. The experimental results qualitatively and quantitatively proved the feasibility of the proposed methodology in improving the geo-referencing accuracy of real datasets collected over an agricultural field.
Z-RAM® ultra-dense memory for 90nm and below (2006)

This article consists of a collection of slides from the author's conference presentation on Innovative Silicon's Z-RAM, an ultra-dense memory processor. Some of the specific topics discussed include: an overview of the device and its system architecture; major processing challenges; and key Z-RAM performance factors, including memory density, speed processing, pipeline operations, routing impact, active power capabilities, and standby power facilities.
Determination of double-bond position in diunsaturated compounds by mass spectrometry of dimethyl disulfide derivatives (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetermination of double-bond position in diunsaturated compounds by mass spectrometry of dimethyl disulfide derivativesMarco. Vincent, Gianfranco. Guglielmetti, Giorgio. Cassani, and Cristina. ToniniCite this: Anal. Chem. 1987, 59, 5, 694–699Publication Date (Print):March 1, 1987Publication History Published online1 May 2002Published inissue 1 March 1987https://pubs.acs.org/doi/10.1021/ac00132a003https://doi.org/10.1021/ac00132a003research-articleACS PublicationsRequest reuse permissionsArticle Views822Altmetric-Citations154LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts