gebhart/patrepeval huggingface dataset:
A protein G-related cell surface protein in Streptococcus zooepidemicus (1995)

This work describes the cloning and sequencing of a gene encoding a plasma protein receptor from Streptococcus zooepidemicus. This receptor, termed protein ZAG, is a 45-kDa protein that binds alpha 2-macroglobulin (alpha 2M), serum albumin, and immunoglobulin G (IgG). The IgG-binding activity is located in the C-terminal part of the molecule and is mediated by two repeated domains highly homologous to each other as well as to the corresponding domains in streptococcal type III Fc receptors. The IgG-binding profile of protein ZAG is similar to that previously reported for S. zooepidemicus. Binding to serum albumin is mediated by a short amino acid sequence in the middle of the molecule. This domain shows homology to previously described albumin-binding proteins from streptococci, and the albumin-binding profile of protein ZAG is similar to that of streptococcal protein G. The N-terminal part of protein ZAG, which mediates binding to the plasma proteinase inhibitor alpha 2M, is composed of a unique stretch of amino acids. Protein ZAG competes for the same, or nearby, binding site(s) in alpha 2M as do two recently described Streptococcus dysgalactiae receptors, although the sequences of the alpha 2M-binding domains in these three receptors show only minor sequence similarities.
Generation and detection of coherent terahertz waves using two photomixers (1998)

A general technique has been demonstrated at microwave and submillimeter-wave frequencies for photoconductive sampling in the frequency domain using photomixers and continuous-wave laser diodes. A microwave version in which two photomixers were coupled by a transmission line was developed to quantitatively test the concept from 0.05 to 26.5 GHz. A quasioptical version using antenna-coupled photomixers was demonstrated from 25 GHz to 2 THz. Such a system can outperform systems based on time-domain photoconductive sampling in frequency resolution, spectral brightness, system size, and cost.
Specific removal of endotoxin from protein solutions by immobilized histidine (1990)

A method for reducing endotoxin contamination in various solutions by immobilized histidine is described. Immobilized histidine is a porous adsorbent suitable for the adsorption of endotoxin with a high affinity over a wide range of pH and temperature and at low ionic strength (gamma/2 less than or equal to 0.1). When a purified endotoxin originating from Escherichia coli UKT-B was studied, the apparent dissociation constant between endotoxin and the adsorbent was 7.3 X 10(-13) M. The adsorbent was able to remove various kinds of endotoxin originating from gram-negative bacteria; the concentration of endotoxin was reduced from 1000 to less than 0.01 ng/ml in water. It is shown that the adsorbent specifically adsorbs endotoxin provided that the adsorption conditions are properly selected. Some examples of the specific removal of endotoxin from high-molecular-weight physiologically active substances such as tumor necrosis factor and lysozyme are shown.
Impaired Mitochondrial Activity in the Insulin-Resistant Offspring of Patients with Type 2 Diabetes (2004)

These data support the hypothesis that insulin resistance in the skeletal muscle of insulin-resistant offspring of patients with type 2 diabetes is associated with dysregulation of intramyocellular fatty acid metabolism, possibly because of an inherited defect in mitochondrial oxidative phosphorylation.
Immunophenotype of Human Adipose-Derived Cells: Temporal Changes in Stromal-Associated and Stem Cell–Associated Markers (2005)

Adipose tissue represents an abundant and accessible source of multipotent adult stem cells and is used by many investigators for tissue engineering applications; however, not all laboratories use cells at equivalent stages of isolation and passage. We have compared the immunophenotype of freshly isolated human adipose tissue-derived stromal vascular fraction (SVF) cells relative to serial-passaged adipose-derived stem cells (ASCs). The initial SVF cells contained colony-forming unit fibroblasts at a frequency of 1:32. Colony-forming unit adipocytes and osteoblasts were present in the SVF cells at comparable frequencies (1:28 and 1:16, respectively). The immunophenotype of the adipose-derived cells based on flow cytometry changed progressively with adherence and passage. Stromal cell-associated markers (CD13, CD29, CD44, CD63, CD73, CD90, CD166) were initially low on SVF cells and increased significantly with successive passages. The stem cell-associated marker CD34 was at peak levels in the SVF cells and/or early-passage ASCs and remained present, although at reduced levels, throughout the culture period. Aldehyde dehydrogenase and the multidrug-resistance transport protein (ABCG2), both of which have been used to identify and characterize hematopoietic stem cells, are expressed by SVF cells and ASCs at detectable levels. Endothelial cell-associated markers (CD31, CD144 or VE-cadherin, vascular endothelial growth factor receptor 2, von Willebrand factor) were expressed on SVF cells and did not change significantly with serial passage. Thus, the adherence to plastic and subsequent expansion of human adipose-derived cells in fetal bovine serum-supplemented medium selects for a relatively homogeneous cell population, enriching for cells expressing a stromal immunophenotype, compared with the heterogeneity of the crude SVF.
TSLP Conditions the Lung Immune Environment for the Generation of Pathogenic Innate and Antigen-Specific Adaptive Immune Responses (2009)

Thymic stromal lymphopoietin (TSLP) is crucial for the development of atopic diseases in humans and mice. Mice that express a lung-specific TSLP transgene (surfactant protein C promoter (SPC)-TSLP) develop a spontaneous and progressive asthma-like disease, suggesting that TSLP expression alone was sufficient for disease development. In this study, we show that, in fact, TSLP alone only causes a weak innate response that is insufficient for development of full airway inflammatory disease. Complete disease development requires both TSLP and antigenic stimulation. These data suggest that the spontaneous lung inflammation observed in SPC-TSLP mice reflects a TSLP-driven predisposition toward the development of aberrant responses against innocuous environmental Ags. This provides evidence that TSLP may act directly to induce susceptibility to the inappropriate allergic responses that characterize atopy and asthma. We additionally show that disease development requires CD4 T cells but not B cells. Further, we reveal a TSLP-driven innate response involving mucus overproduction and goblet cell metaplasia. Taken together, these data suggest a multifaceted model of TSLP-mediated airway inflammation, with an initial activation of resident innate immune cells, followed by activation of the adaptive immune system and full disease development. This study provides new insight into the unique features of the asthma pathology contributed by the innate and adaptive immune responses in response to TSLP stimulation.
Mucoadhesion; A prerequisite or a constraint in nasal drug delivery? (2011)

Nasal drug administration has frequently been proposed as the most feasible alternative to parenteral injections. This is due to the high permeability of the nasal epithelium, allowing a higher molecular mass cut-off at approximately 1000 Da and the rapid drug absorption rate with plasma drug profiles sometimes almost identical to those from intravenous injections. Nasal drug administration is presently used for local therapies within the nasal cavity. Anti-allergic drugs and nasal decongestants are the most common examples. However, nasal drug administration for systemic effects has been practiced since ancient times. Nasallyadministered psychotropic drugs by native Indians, the use of tobacco snuffs, and nasal administration of illicit drugs such as cocaine are all well known. Read more...
Mutations in DNA2 Link Progressive Myopathy to Mitochondrial DNA Instability (2013)

Syndromes associated with multiple mtDNA deletions are due to different molecular defects that can result in a wide spectrum of predominantly adult-onset clinical presentations, ranging from progressive external ophthalmoplegia (PEO) to multisystemic disorders of variable severity. The autosomal-dominant form of PEO is genetically heterogeneous. Recently, causative mutations have been reported in several nuclear genes that encode proteins of the mtDNA replisome machinery (POLG, POLG2, and C10orf2) or that are involved in pathways for the synthesis of deoxyribonuclotides (ANT1 and RRM2B). Despite these findings, putative mutations remain unknown in half of the subjects with PEO. We report the identification, by exome sequencing, of mutations in DNA2 in adult-onset individuals with a form of mitochondrial myopathy featuring instability of muscle mtDNA. DNA2 encodes a helicase/nuclease family member that is most likely involved in mtDNA replication, as well as in the long-patch base-excision repair (LP-BER) pathway. In vitro biochemical analysis of purified mutant proteins revealed a severe impairment of nuclease, helicase, and ATPase activities. These results implicate human DNA2 and the LP-BER pathway in the pathogenesis of adult-onset disorders of mtDNA maintenance.
Integrated optical interferometric detection method for micromachined capacitive acoustic transducers (2002)

An integrated optical interferometric detection method for micromachined capacitive acoustic transducers is reported. The back electrode of the capacitive transducer on a transparent substrate is shaped as an optical diffraction grating and the displacement of the transducer membrane is determined with interferometric resolution by measuring the intensity of the reflected diffraction orders. By applying voltage to deflect the membrane electrostatically, the detection sensitivity is kept at the optimum level and transmission signals are generated. Initial experiments on devices microfabricated on quartz substrates show a minimum detectable displacement of 2×10−4 Å/√Hz around 250 kHz and low frequency detection capability for microphone applications. Ultrasonic pulse-echo experiments in air at 750 kHz are also demonstrated using both a HeNe laser and a 850 nm vertical cavity surface emitting laser as the light source.
Detection and biochemical characterization of antigens in human leukemic sera that cross-react with primate C-type viral proteins (Mr 30,000). (1983)

Antigens have been detected in 35 to 40% of sera from patients with leukemia that cross-react with the Mr 30,000 core proteins (p30) of baboon endogenous virus (BaEV) and/or of simian sarcoma-simian sarcoma-associated virus (SiSV) in a solid-phase enzyme immunoassay using anti-SiSV p30 and anti-BaEV p30 antisera. These antigens could not be found in sera from nonleukemic persons. Fetal calf serum; normal chicken, goat, rabbit, and rhesus sera; and normal human serum components like albumin, immunoglobulins, and transferrin did not react with the anti-SiSV p30 and anti-BaEV p30 antisera. The reactivity in the leukemic sera was abolished by treatment with protease, but not with glycosidases. The antigens purified by immunoaffinity chromatography showed essentially one band with an apparent molecular weight of 70,000 on sodium dodecyl sulfate: polyacrylamide gel electrophoresis. In competition enzyme-linked immunosorbent assays the leukemia-associated antigens competed out SiSV p30 in the anti-SiSV p30 system. Peptide mapping experiments with antigens from sera of two different leukemic patients showed that the two antigens were identical concerning numbers of peptides and their position. Eleven of 21 major peptides of SiSV p30 and 10 of 20 major peptides of BaEV p30 (50 to 60% of major peptides) showed mobilities identical with those of the major peptides of the human antigens. The data suggest the presence in human sera of retroviral antigens closely associated with leukemia.
Defining cellular senescence in IMR-90 cells: a flow cytometric analysis. (1988)

Using multiparameter flow cytometric analysis, we find that senescent cells accumulate in a unique cell-cycle compartment characterized in cell-cycle arrest in G1 and a significantly reduced nucleocytoplasmic ratio (genome size/cell mass) relative to cycling cells. With respect to gross cellular phenotype, the quiescent state of senescent cells differs from quiescence induced by density inhibition; the former is associated with a reduction in the nucleocytoplasmic ratio, while the latter is associated with an increase in the nucleocytoplasmic ratio. Senescent cells were present at all passages examined. The frequency of senescent cells was low in early-passage cultures and increased with passage number. Senescence of populations of IMR-90 cells reflects change in the relative frequency of these cells. The frequency of cells with karyotypic changes increased with the progressive accumulation of out-of-cycle cells.
Obstructive Apneic Events Induce Alpha-receptor Mediated Digital Vasoconstriction (2004)

OSA related alteration of the pulse amplitude includes a constriction of digital skin vasculature that to a large extent is mediated via sympathoadrenergic alpha-receptors.
Influence of Exogenous Bovine Somatotropin on the Responsiveness of Peripheral Blood Lymphocytes to Mitogen (1991)

Our objectives were to determine the effects of exogenous bovine somatotropin on peripheral blood lymphocyte responsiveness to mitogen and on concentrations of serum insulin-like growth factor-I and to determine whether a statistical relationship exists between these two variables. The cows on trial were treated with 0, 10.3, or 20.6 mg/d somatotropin, starting between wk 4 and 5 of lactation and continuing for 266 consecutive d. Lymphocyte proliferation (counts per minute) and concentration of insulin-like growth factor-I (nanograms per milliliter) were recorded from blood sampled at wk 3, 10, 26, 35, and 46 of lactation. The results showed that lymphocytes from the blood of treated cows responded to mitogen with higher proliferative responsiveness than cells from control cows, but that this effect required long-term treatment and adequate mitogen concentrations to be detected. The blood of treated cows also had elevated concentrations of insulin-like growth factor-I relative to that of control cows, but these were not statistically associated with the proliferative responsiveness of the peripheral blood lymphocytes to mitogen. Our results suggest that somatotropin has a role in the immune system of cows, but its mode of action and target tissues must be determined. Bovine somatotropin, injected at doses that increase milk yield, can augment cow immunity as judged by the proliferative responsiveness of peripheral blood lymphocytes in culture.
Phase I clinical evaluation of a new murine monoclonal antibody (mu-9) against colon-specific antigen-p for targeting gastrointestinal carcinomas (1994)

These initial results support the need for further evaluation of Mu-9 immunoglobulin G and F(ab')2 for targeting gastrointestinal cancer for radioimmunotherapy.
Advanced engineering electromagnetics (1989)

Time--Varying and Time--Harmonic Electromagnetic Fields. Electrical Properties of Matter. Wave Equation and Its Solutions. Wave Propagation and Polarization. Reflection and Transmission. Auxiliary Vector Potentials, Contruction of Solutions, and Radiation and Scattering Equations. Electromagnetic Theorems and Principles. Rectangular Cross--Section Waveguides and Cavities. Circular Cross--Section Waveguides and Cavities. Spherical Transmission Lines and Cavities. Scattering. Integral Equations and the Moment Method. Geometrical Theory of Diffraction. Greena s Functions. Appendices. Index.
Efficient bump mapping hardware (1997)

Article Free Access Share on Efficient bump mapping hardware Authors: Mark Peercy Silicon Graphics Computer Systems, 2011 N. Shoreline Boulevard, Mountain View, California Silicon Graphics Computer Systems, 2011 N. Shoreline Boulevard, Mountain View, CaliforniaView Profile , John Airey Silicon Graphics Computer Systems, 2011 N. Shoreline Boulevard, Mountain View, California Silicon Graphics Computer Systems, 2011 N. Shoreline Boulevard, Mountain View, CaliforniaView Profile , Brian Cabral Silicon Graphics Computer Systems, 2011 N. Shoreline Boulevard, Mountain View, California Silicon Graphics Computer Systems, 2011 N. Shoreline Boulevard, Mountain View, CaliforniaView Profile Authors Info & Claims SIGGRAPH '97: Proceedings of the 24th annual conference on Computer graphics and interactive techniquesAugust 1997 Pages 303–306https://doi.org/10.1145/258734.258873Published:03 August 1997Publication History 58citation1,156DownloadsMetricsTotal Citations58Total Downloads1,156Last 12 Months56Last 6 weeks5 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Metabolic persistence of fetal hemoglobin (1995)

Hereditary persistence of fetal hemoglobin (HPFH) has typically been ascribed to mutations in the beta-globin gene cluster. Pharmacologic agents, including the short-chain fatty acid butyrate, have been shown to upregulate fetal and embryonic globin gene expression. In this report we investigate the possibility that metabolic derangements characterized by an inability to metabolize another short-chain fatty acid, propionate, could be associated with a persistence of fetal hemoglobin unrelated to alterations in the beta-globin cluster. Embryonic globin gene upregulation in a murine adult erythroid cell culture was shown by RNase protection after induction with three short-chain fatty acids (C2-C5). Chart reviews and measurement of fetal hemoglobin in five patients with abnormalities in propionate (C3) metabolism were undertaken; SSCP/dideoxy fingerprint analysis of the gamma-globin gene promoters was done in three of these five patients. Twelve patients with other metabolic derangements served as controls. Only the four patients with clinically severe abnormalities in propionate metabolism (ages 2 to 11), but without anemia, showed a sustained elevation in fetal hemoglobin (3% to 10%). The level of elevation of fetal hemoglobin in these patients, who lack erythropoietic stress, suggests that propionic acid and/or its metabolites are potent stimulators of fetal hemoglobin expression. Study of this group of patients should allow unique insights into the long-term effects of sustained exposure to elevations of short-chain fatty acid levels.
Complex Regional Pain Syndromes: Guidelines for Therapy (1998)

This report aims to present an orderly approach to the treatment of Chronic Regional Pain Syndrome (CRPS) types I and II through an algorithm. The central theme is functional restoration: a coordinated but progressive approach that introduces each of the treatment modalities needed to achieve both remission and rehabilitation. Reaching objective and measurable rehabilitation goals is an essential element. Specific exercise therapy to reestablish function after musculoskeletal injury is central to this functional restoration. Its application to CRPS is more contingent on varying rates of progress that characterize the restoration of function in patients with CRPS. Also, the various modalities that may be used, including analgesia by pharmacologic means or regional anesthesia or the use of neuromodulation, behavioral management, and the qualitatively different approaches that are unique to the management of children with CRPS, are provided only to facilitate functional improvement in a stepwise but methodical manner. Patients with CRPS need an individual approach that requires extreme flexibility. This distinguishes the management of these conditions from other well-described medical conditions having a known pathophysiology. In particular, the special biopsychosocial factors that are critical to achieving a successful outcome are emphasized. This algorithm is a departure from the contemporary heterogeneous approach to treatment of patients with CRPS. The underlying principles are motivation, mobilization, and desensitization facilitated by the relief of pain and the use of pharmacologic and interventional procedures to treat specific signs and symptoms. Self-management techniques are emphasized, and functional rehabilitation is the key to the success of this algorithm.
Text Classification Using WordNet Hypernyms (1998)

This paper describes experiments in Machine Learning for text classification using a new representation of text based on WordNet hypernyms. Six binary classification tasks of varying difficulty are defined, and the Ripper system is used to produce discrimination rules for each task using the new hypernym density representation. Rules are also produced with the commonly used bag-of-words representation, incorporating no knowledge from WordNet. Experiments show that for some of the more difficult tasks the hypernym density representation leads to significantly more accurate and more comprehensible rules. 1. Introduction The task of Supervised Machine Learning can be stated as follows: given a set of classification labels C, and set of training examples E, each of which has been assigned one of the class labels from C, the system must use E to form a hypothesis that can be used to predict the class labels of previously unseen examples of the same type [Mitchell 97]. In machine learnin...
Data Communications, Computer Networks, and Open Systems (1996)

Part One: Data Communications 1. Data communication networks and open system standards 2. The electrical interface 3. Data transmission 4. Protocol basics 5. Data link control protocols Part Two: Computer Networks 6. Local area networks 7. High-speed and bridged local area networks 8. Wide area networks 9. Internetworking 10. Broadband multiservice networks Part Three: Open Systems 11. Transport protocols 12. Application support protocols 13. Application-specific protocols 14. System aspects Appendices Bibliography Index
Principles for the Buffering of Genetic Variation (2001)

Most genetic research has used inbred organisms and has not explored the complexity of natural genetic variation present in outbred populations. The translation of genotype to phenotype is complicated by gene interactions observed as epistasis, canalization, robustness, or buffering. Analysis of double mutations in inbred experimental organisms suggests some principles for gene interaction that may apply to natural variation as well. The buffering of variation in one gene is most often due to a small number of other genes that function in the same biochemical process. However, buffering can also result from genes functioning in processes extrinsic to that of the primary gene.