gebhart/patrepeval huggingface dataset:
Nonlinear optical properties of a new chromophore (1997)

Strong two-photon-absorption (TPA) based nonlinear optical properties of a new chromophore, N, N-diphenyl-7-[2-(4-pyridinyl) ethenyl]-9,9-di-n-decyl-fluoren-2-amine (AF-50) has been experimentally studied. Under excitation with 10-Hz, ∼8-ns, and ∼800-nm laser pulses, the TPA cross section and the TPA-induced frequency-upconverted emission spectra are measured for AF-50 solutions in various solvents. The most attractive feature of this chromophore is its remarkably high value of the molecular TPA cross section (∼78×10-20 cm2/GW in benzene solution). Based on this feature, superior optical power limiting and stabilization performance has been demonstrated in a 1-cm-long AF-50 solution sample with concentration of d0=0.045 M/L. The nonlinear transmission of the measured sample decreased from ∼0.93 to ∼0.3 when the input-beam intensity increased from ∼10 MW/cm2 to ∼360 MW/cm2; the relative intensity fluctuation of the output laser pulses was reduced to one third of that of the input laser beam.
Cobalt to oxygen migration of the trimethylsilyl group in trimethylsilylcobalt tetracarbonyl (1973)

When Me3Si–Co(CO)4 is heated at 105° for 50 h, migration of the Me3Si group from cobalt to oxygen takes place to give Me3SiOCCo3(CO)9 and (Me3SiOC)4Co2(CO)4; the former compound is also formed in high yields within an hour at room temperature by the reaction of Me3SiCo(CO)4 with tetrahydrofuran.
Experimental study on antitumour effect of CA4P (2009)

OBJECTIVE To observe the inhibitory effect of Combretaftation A4 prodrug( CA4P ) on human cervical carcinoma Hela cell in vitro and in nude mice. METHODS The inhibitory effect of CA4P human cervical carcinoma Hela cell was investigated by MTT analysis. Hela cells were inoculated subcutaneously into nude mice. Volumes of tumor were measured and growth of tumor was observed and the inhibitory rate of tumor growth was computed. RESULTS After treated with CA4P for 24 or 48 hours,MTT assay showed the dose range from 0. 5μg·mL -1 to 64 μg·mL -1 had the inhibitory ratio range from 15% to 75% and from 18% to 80% respectively. The IC50 was 0. 336 μg·mL -1 and 0. 207 μg·mL -1,respectively. The inhibitory rate of tumor growth in nude mice carried tumour was 30% to 50% ,and it was related with the dose. CONCLUSION CA4P could inhibit the growth of human cervical carcinoma Hela cell in vitro and in vivo.
HIGH-SPEED ELECTROSLAG WELDING (1984)

High-speed electroslag welding, in which filler metal in powder form is added, offers advantages in productivity and in notch-toughness and other mechanical properties without postweld heat-treatment. Various electrode and metal-powder feeding systems have been developed for different plate thicknesses and different joint preparations, and ensure reliable welding and stable process conditions. The authors, of the Technische Hochschule, Aachen, explain the techniques used in this process, describe some of the equipment used, and discuss the advantages of the process over conventional electroslag welding. It is considered that higher toughness values that will be demanded from electroslag welding will in the future require high-speed methods and the use of steels that are resistant to high energy inputs; at the same time, automation of the welding process should ensure consistently good welding.
Expression of aquaporin 5 in primary carcinoma and lymph node metastatic carcinoma of non-small cell lung cancer (2015)

Aquaporin 5 (AQP5), a water channel protein, is highly expressed in non-small cell lung cancer (NSCLC) tissues compared with adjacent normal tissues. AQP5 expression in lung cancer tissues is associated with a poor prognosis. The present study aimed to analyze the expression of AQP5 and investigate its role in primary and lymph node metastatic NSCLCs. An immunohistochemical labeled streptavidin-biotin method was used to determine the expression of AQP5 in 94 cases of NSCLC primary carcinoma, which included 51 cases accompanied by lymph node metastasis. The results revealed that the expression of AQP5 was significantly higher in adenocarcinomas compared with squamous cell carcinomas (P=0.002). In addition, the percentage of AQP5 expression in the primary carcinomas with lymph node metastasis was significantly higher compared with those without lymph node metastasis (P=0.024). However, no statistically significant difference in the percentage of AQP5 expression was observed between the metastatic and the primary carcinomas (P=0.377). The expression of AQP5 exhibited a correlation with the tumor-node-metastasis staging of NSCLC (P=0.027). The percentage of AQP5 expression in stage III and IV tumors was higher than that in stage I and II tumors. In addition, AQP5 expression was correlated with the survival rate of NSCLC patients (P=0.051). In conclusion, the results of the present study provide evidence for the AQP5-facilitated incidence, progression and metastasis of NSCLC. Therefore, AQP5 may be used as a potential target to investigate the incidence, progression and metastasis of NSCLC.
Dynamics of Seed-Borne Rice Endophytes on Early Plant Growth Stages (2012)

Bacterial endophytes are ubiquitous to virtually all terrestrial plants. With the increasing appreciation of studies that unravel the mutualistic interactions between plant and microbes, we increasingly value the beneficial functions of endophytes that improve plant growth and development. However, still little is known on the source of established endophytes as well as on how plants select specific microbial communities to establish associations. Here, we used cultivation-dependent and -independent approaches to assess the endophytic bacterrial community of surface-sterilized rice seeds, encompassing two consecutive rice generations. We isolated members of nine bacterial genera. In particular, organisms affiliated with Stenotrophomonas maltophilia and Ochrobactrum spp. were isolated from both seed generations. PCR-based denaturing gradient gel electrophoresis (PCR-DGGE) of seed-extracted DNA revealed that approximately 45% of the bacterial community from the first seed generation was found in the second generation as well. In addition, we set up a greenhouse experiment to investigate abiotic and biotic factors influencing the endophytic bacterial community structure. PCR-DGGE profiles performed with DNA extracted from different plant parts showed that soil type is a major effector of the bacterial endophytes. Rice plants cultivated in neutral-pH soil favoured the growth of seed-borne Pseudomonas oryzihabitans and Rhizobium radiobacter, whereas Enterobacter-like and Dyella ginsengisoli were dominant in plants cultivated in low-pH soil. The seed-borne Stenotrophomonas maltophilia was the only conspicuous bacterial endophyte found in plants cultivated in both soils. Several members of the endophytic community originating from seeds were observed in the rhizosphere and surrounding soils. Their impact on the soil community is further discussed.
Biosensors based on surface plasmon-enhanced fluorescence spectroscopy (Review) (2008)

The implementation of surface plasmon-enhanced fluorescence spectroscopy (SPFS) to surface plasmon resonance (SPR) biosensors enables increasing their sensitivity by several orders of magnitude. In SPR-based biosensors, surface plasmons probe the binding of target molecules contained in a liquid sample by their affinity partners attached to a metallic sensor surface. SPR biosensors relying on the detection of refractive index changes allow for direct observation of the binding of large and medium size molecules that produces sufficiently large refractive index changes. In SPR biosensors exploiting SPFS, the capture of fluorophore-labeled molecules to the sensor surface is observed by the detection of fluorescence light emitted from the surface. This technique takes advantage of the enhanced intensity of electromagnetic field accompanied with the resonant excitation of surface plasmons. The interaction with surface plasmons can greatly increase the measured fluorescence signal through enhancing the excitation rate of fluorophores and by more efficient collecting of fluorescence light. SPFS-based biosensors were shown to enable the analysis of samples with extremely low analyte concentrations and the detection of small molecules. In this review, we describe the fundamental principles, implementations, and current state of the art applications of SPFS biosensors. This review focuses on SPFS-based biosensors employing the excitation of surface plasmons on continuous metal-dielectric interfaces.
Use of substituted and tandem‐repeated peptides to probe the relevance of the highly conserved RGD tripeptide in the immune response against foot‐and‐mouth disease virus (1993)

Antigenic site A of foot-and-mouth disease virus (FMDV) is an exposed, mobile loop which includes a central, highly conserved Arg-Gly-Asp tripeptide (RGD, VP1 residues 141-143 in serotype C) thought to be part of the cell attachment site. We have analyzed the contribution of RGD to the interaction of site A with antibodies by incorporating selected amino acid replacements at RGD into synthetic peptides representing site A, and analyzing the reactivity of substituted peptides with site A-specific monoclonal antibodies (MAbs). Replacement of Arg-141, Gly-142 or Asp-143 by alanine resulted in the loss of one, three and five epitopes, respectively, out of seven epitopes probed. Other replacements resulted in the loss of even larger numbers of epitopes, suggesting that the amino acids of the RGD region are either directly involved in interaction with antibodies or that they exert an important influence on the interaction of surrounding residues with antibodies. Thus, we explored the ability of tandem repeats of the RGDL sequence (corresponding to FMDV C-S8c1) to evoke neutralizing antibodies in rabbits and guinea pigs. Neutralizing activity was generally low but with a broad specificity for different FMDV serotypes and variants. Significant decreases in neutralizing titers were observed with boosting, suggesting a possible suppression of those anti-peptide antibodies which may also be directed to cellular RGD sequences. The results point to an involvement of RGD in the antigenic structure of site A, and open the possibility that broadly neutralizing antibodies might be induced by tandem repeats of the critical, conserved domain.
Probing three-dimensional structure of bovine serum albumin by chemical cross-linking and mass spectrometry (2004)

Serum albumin is the principal transporter of fatty acids that are otherwise insoluble in circulating plasma. While the crystal structure of human serum albumin (HSA) as well as its binding with fatty acids has been characterized, the three dimensional structure of bovine serum albumin (BSA) has not been determined although both albumins share 76% sequence homology. In this study we used mass spectrometry coupled with chemical cross-linking, to probe the tertiary structure of BSA. BSA was modified with lysine specific cross-linkers, bis(sulfosuccinimidyl) suberate (BS(3)), disuccinimidyl suberate (DSS) or disuccinimidyl glutarate (DSG), digested with trypsin and analyzed by tandem mass spectrometry. With O-18 labeling during the digestion, through-space cross-linked peptides were readily identified in mass spectra by a characteristic 8 Da shift. From the cross-linked peptides identified in this study, we found that 12 pairs of lysine residues were separated within 20 A, while 5 pairs were spaced between 20 and 24 A. The spatial distance constraints generated from five K-K pairs in BSA were consistent with the corresponding distance obtained from the crystal structure of HSA, although only six equivalent K-K pairs could be compared. According to our data, the distance between K235 of IIA and K374 of IIB domain in BSA was farther by 7-11 A than that expected from the crystal structure of HSA, suggesting structural differences between BSA and HSA in this region. The distance constraints obtained for lysine residues using various cross-linkers should be valuable in assisting the determination of the 3-D structure of BSA.
Improvement of the critical current density in the silver sheathed Bi-Pb-Sr-Ca-Cu-O superconducting tape (1989)

The critical current density (Jc) at 77 K and in 0 T of more than 3000 A/cm2 has been obtained in the silver sheathed Bi-Pb-Sr-Ca-Cu-O tape fabricated by the combination process of sintering and intermediate cold working. The decrease of the Jc with increasing magnetic field is much smaller in the tape prepared by this process than that prepared by sintering only. The improvement of the Jc is attributed to the improvement of the coupling between the grains of the high Tc phase. The process enhances the formation of the high Tc phase and produces a highly oriented grain structure. These are considered to be responsible for the large improvement of the coupling.
<title>Laser direct writing of microbatteries for integrated power electronics</title> (2001)

A novel laser-based process developed at the Naval Research Laboratory has been used to fabricate pseudocapacitors and microbatteries with tailored capacities for small electronic devices having size and/or weight restrictions. This process, called MAPLE DW (for matrix-assisted pulsed-laser evaporation direct write) can deposit rugged mesoscale (1 micrometers to 10 mm) electronic components over any type of substrate. A pulsed laser operating at 355 nm is used to forward transfer material from a tape-cast ribbon to a suitable substrate to form a precision design. With MAPLE DW, customized mesoscale electronic components can be produced, eliminating the need for multiple fabrication techniques and surface-mounted components. Direct write processing is especially attractive for the fabrication of micro-power sources and systems. The versatility of laser processing allows battery designs to be easily modified. Batteries and/or pseudocapacitors can be integrated with power management electronics to deliver a wide range of power outputs. By building power sources directly on electronic components, the weight of the power sources is decreased as the electronic substrate becomes part of the battery packaging and the lengths of interconnects are shortened, reducing conductor losses. RuOxHy pseudocapacitors deposited with MAPLE DW show good storage capacities. Pads of hydrous RuO<SUB>2</SUB> having dimensions of 2.2 mm x 1.0 mm x 30 micrometers have been deposited in a planar configuration on gold current collectors. Rechargeable Zn/MnO<SUB>2</SUB> alkaline microbatteries comprising of MnO<SUB>2</SUB>, Zn, an ethyl cellulose separator barrier layer and a KOH electrolyte have also been fabricated by MAPLE DW. The resulting structures with dimensions of 1.5 mm x 1.5 mm x 60 micrometers represent the first demonstration of a multilayer microbattery made by MAPLE DW. The performance of these prototypes are shown and the potential impact of MAPLE DW for the fabrication of novel microbattery systems for integrated power applications are discussed.
Antithrombotic and hemorrhagic effects of DX-9065a, a direct and selective factor Xa inhibitor: comparison with a direct thrombin inhibitor and antithrombin III-dependent anticoagulants. (1997)

(+)-2S-2-[4-[[(3S)-1-Acetimidoyl-3- pyrrolidinyl]oxy]phenyl]-3-[7-amidino-2-naphthyl]propanoic acid hydrochloride pentahydrate (DX-9065a) is an antithrombin III (AT III)-independent and selective inhibitor of activated blood coagulation factor X (FXa). The aim of the present study was to compare the antithrombotic and hemorrhagic effects of DX-9065a with a direct thrombin inhibitor and AT III-dependent anticoagulants in rat models of thrombosis and bleeding. Rats were administered intravenously DX-9065a (0.1-1 mg/kg/h), argatroban (0.1-1 mg/k/h), low molecular weight heparin (25-100 anti-XaU/kg/h), unfractionated heparin (25-100 anti-XaU/kg/h) or Orgaran (30-300 anti-XaU/kg/h) for 1 h. DX-9065a dose-dependently inhibited both thrombus formation and elevation in plasma thrombin-AT III complex (TAT) level in a copper wire-inserted arteriovenous (AV) shunt model in rats. The dose required for 50% inhibition of thrombus formation was 0.27 mg/kg/h. DX-9065a did not prolong transection bleeding time up to 7.78 mg/kg/h. Argatroban and AT III-dependent anticoagulants also inhibited both thrombus formation and TAT elevation, but prolonged bleeding time at a slightly higher dose than the effective dose. These results suggest that direct and selective inhibition of factor Xa by DX-9065a is preferable for the treatment of thrombosis in the aspect of lack of compromising primary hemostasis.
Photoimmunotheranostic agents for triple-negative breast cancer diagnosis and therapy that can be activated on demand (2016)

Triple-negative breast cancer (TNBC) is a heterogeneous disease in which the tumors do not express estrogen receptor (ER), progesterone receptor (PgR) or human epidermal growth factor receptor 2 (HER2). Classical receptor-targeted therapies such as tamoxifen or trastuzumab are therefore unsuitable and combinations of surgery, chemotherapy and/or radiotherapy are required. Photoimmunotheranostics is a minimally invasive approach in which antibodies deliver nontoxic photosensitizers that emit light to facilitate diagnosis and produce cytotoxic reactive oxygen species to induce apoptosis and/or necrosis in cancer cells. We developed a panel of photoimmunotheranostic agents against three TNBC-associated cell surface antigens. Antibodies against epidermal growth factor receptor (EGFR), epithelial cell adhesion molecule (EpCAM) and chondroitin sulfate proteoglycan 4 (CSPG4) were conjugated to the highly potent near-infrared imaging agent/photosensitizer IRDye®700DX phthalocyanine using SNAP-tag technology achieving clear imaging in both breast cancer cell lines and human biopsies and highly potent phototherapeutic activity with IC50values of 62-165 nM against five different cell lines expressing different levels of EGFR, EpCAM and CSPG4. A combination of all three reagents increased the therapeutic activity against TNBC cells by up to 40%.
[Current drug therapy of benign prostatic hyperplasia]. (1996)

Almost 60% of elderly men suffer from symptoms of BPH, which have significant impact on their daily lives. Transurethral resection of the prostate (TURP) is currently the most effective therapy for relieve of obstruction and obstructive symptoms. Morbidity of TURP and disappointing results in patients with mild or moderate symptoms make surgery not suitable for all patients. 2 groups of drugs were currently developed, and showed efficacy in double-blinded placebo-controlled studies: alpha-blocking agents and 5 alpha-reductase inhibitors. Inhibition of alpha-adrenoreceptors significantly increases urinary flow rates and improves symptoms of BPH. Long-acting drugs, who selectively block alpha 1-adrenoreceptors (terazosin, doxazosin, tamsulosin) have the advantage, when compared with non-selective-alpha-blockers, that they have generally less adrenergic side effects. After intake alpha-adrenorceptor antagonists develop almost immediate action. 5 alpha-reductase inhibitors (finasteride) reduce prostatic size by 27%, they increase urinary flow rates and improve prostatic symptom scores, whilst adverse effects are extremely low. Full medical action is after 4 to 6 weeks. Both, alpha-Blockers and 5 alpha-reductase inhibitors need permanent administration for maintenance of action. Yet, synergistic effects, using their different modes of action have not been demonstrated after application of both drugs. Herbal products have not proved efficacious inspite of singular surprising results in clinical trials, their efficacy to treat BPH related symptoms was classified as placebo alike. Prior to therapy urological diagnosis and exclusion of prostate cancer is mandatory. Pharmacotherapy with alpha 1-adrenoreceptor antagonists and 5 alpha-reductase inhibitors have a place in the management of BPH patients with mild to moderate disease, who are bothered by their symptoms, or for those awaiting or wishing to delay surgery.
Increased sensitivity of CLL‐derived lymphocytes to apoptotic death activation by the proteasome‐specific inhibitor lactacystin (1999)

Ubiquitin-proteasome-dependent protein processing appears to be an essential component in the control of radiation-induced apoptosis in human lymphocytes. This control is altered in chronic lymphocytic leukaemia (CLL), compared to that of normal human lymphocytes which mainly showed high apoptotic values after irradiation, but in some cases no sensitivity was observed. Interestingly, lactacystin activated the apoptotic pathway in both radio-resistant and sensitive CLL cells, at doses which had no effect in normal cells where significantly higher concentrations were required. Therefore the resistance of some CLL cells to apoptosis initiation by radiation does not correlate to observed increased sensitivity to lactacystin. The nuclear level of the transcription factor NF-kappaB or the cytoplasmic level of IkappaBalpha remained unaltered upon irradiation or lactacystin CLL cells treatment, suggesting that the activity of the other factors involved in apoptotic death control were altered through proteasomal inhibition. These results strongly suggest an essential role of the ubiquitin system in apoptotic cell death control in CLL lymphocytes. The inhibition of proteasome-ubiquitin-dependent processing could be a discriminatory apoptotic stimulus between normal versus malignant lymphocytes and therefore might potentially be of use in this specific human pathology.
The Electrohydrodynamic Atomization of Liquids (1986)

The mechanism by which resistive liquids subjected to high electric potentials produce a filament and aerosol is still not well-understood. An experimental study of the onset parameters of this electrohydrodynamic (EHD) atomization mode is presented. Data on the effect of onset potential, capillary radius, liquid conductivity, and viscosity, together with an observed hysteresis in the current-voltage characteristics, provide the basis for a qualitative model of the phenomena presented here for the first time. The flow of liquid that produces the aerosol is understood in terms of the surface stress tensor.
Determination of the Role of the Human RNase H1 in the Pharmacology of DNA-like Antisense Drugs (2004)

Although ribonuclease H activity has long been implicated as a molecular mechanism by which DNA-like oligonucleotides induce degradation of target RNAs, definitive proof that one or more RNase H is responsible is lacking. To date, two RNase H enzymes (H1 and H2) have been cloned and shown to be expressed in human cells and tissues. To determine the role of RNase H1 in the mechanism of action of DNA-like antisense drugs, we varied the levels of the enzyme in human cells and mouse liver and determined the correlation of those levels with the effects of a number of DNA-like antisense drugs. Our results demonstrate that in human cells RNase H1 is responsible for most of the activity of DNA-like antisense drugs. Further, we show that there are several additional previously undescribed RNases H in human cells that may participate in the effects of DNA-like antisense oligonucleotides.