gebhart/patrepeval huggingface dataset:
Ceramide 1-phosphate, a novel phospholipid in human leukemia (HL-60) cells. Synthesis via ceramide from sphingomyelin. (1990)

Prior studies demonstrated that conversion of sphingomyelin to ceramide via sphingomyelinase action resulted in the generation of free sphingoid bases and inactivation of protein kinase C in human leukemia (HL-60) cells (Kolesnick, R. N. (1989) J. Biol. Chem. 264, 7617-7623). The present studies define the novel phospholipid ceramide 1-phosphate in these cells and present evidence for formation of this compound by preferential utilization of ceramide derived from spingomyelin. A ceramide 1-phosphate standard, prepared enzymatically via diacylglycerol kinase, was utilized for localization. In cells labeled to equilibrium with 32Pi to label the head group of the molecule, the basal ceramide 1-phosphate level was 30 +/- 2 pmol/10(6) cells. Generation of ceramide via the use of exogenous sphingomyelinase resulted in time- and concentration-dependent formation of ceramide 1-phosphate. As little as 3.8 x 10(-5) units/ml was effective and a 3-fold increase was observed with a maximal concentration of 3.8 x 10(-2) units/ml; ED50 approximately 2 x 10(-4) units/ml. This effect was observed by 5 min and maximal at 30 min. Similarly, in cells labeled with [3H]serine to probe the sphingoid base backbone, the basal level of ceramide 1-phosphate was 39 +/- 5 pmol/10(6) and increased 2.5-fold with sphingomyelinase; ED 50 approximately 5 x 10(-5) units/ml. To determine the source of the phosphate moiety, studies were performed with cells short term labeled with 32Pi and resuspended in medium without radiolabel. Under these conditions, sphingomyelin was virtually unlabeled. Nevertheless, sphingomyelin (3.8 x 10(-2) units/ml) induced a 12-fold increase in radiolabel incorporation, suggesting ceramide 1-phosphate formation occurred via ceramide phosphorylation. This event appeared specific for ceramide derived from sphingomyelin since ceramide from glycosphingolipids was not converted to ceramide 1-phosphate. In sum, these studies demonstrate the novel phospholipid ceramide 1-phosphate in HL-60 cells and suggest the possibility that a path exists from sphingomyelin to ceramide 1-phosphate via the phosphorylation of ceramide.
Blind equalization in the presence of jammers and unknown noise: solutions based on second-order cyclostationary statistics (1998)

Addresses the blind identification of a linear time-invariant channel using some second-order cyclostationary statistics. In contrast to other contributions, the case where the second-order statistics of the noise and of the jammers are totally unknown is considered. It is shown that the channel can be identified consistently by adapting the so-called subspace method of Moulines et al. (1995). This adaptation is valid for fractionally spaced systems and, more interestingly, for the general systems exhibiting transmitter induced cyclostationarity introduced by Tsatsanis and Glannakis (1995). The new subspace method is based in both cases on a common tool, i.e., a general spectral factorization algorithm. The identifiability conditions are specified and some simulation examples are given.
Expression and Localization of Tissue Factor Pathway Inhibitor-2 in Normal and Atherosclerotic Human Vessels (2002)

Tissue factor pathway inhibitor-2 (TFPI-2) is a Kunitz-type, serine protease inhibitor with inhibitory activity toward activated factor XI, plasma kallikrein, plasmin, certain matrix metalloproteinases, and the tissue factor:activated factor VII complex. In this study, we investigated TFPI-2 expression and localization in normal and atherosclerotic human arteries by using in situ hybridization and immunohistochemical techniques. In healthy human blood vessels, TFPI-2 was detected in the vascular endothelium alone. In human atherosclerotic tissues, TFPI-2 expression was assigned to macrophages, T cells, endothelial cells, and smooth muscle cells. Western blot analysis for TFPI-2 confirmed its production by cultured human aortic smooth muscle cells, U937 cells (monocytes), and Jurkat (T cell) cell lines. Reverse transcription-polymerase chain reaction revealed similar TFPI-2 expression levels in both monocytes and macrophages in culture. Electron microscopic study with immunogold labeling revealed the association of TFPI-2 antigen with both the extracellular matrix and plasma membranes. TFPI-2 antigen was detected in some areas of atheroma that also stained positively for both tissue factor and factor VII. Moreover, detection of TFPI-2 in close spatial proximity to plasmin/plasminogen on macrophages, on endothelial cells, and in matrix-rich areas highlighted its possible functional significance in the regulation of plasmin activity and downstream proteolytic mechanisms that occur in the atherosclerotic lesion.
Asymmetric Catalysis with Chiral Ferrocene Ligands (2003)

Chiral ferrocene ligands have been widely used in asymmetric catalysis. The advantages of using ferrocene as a scaffold for chiral ligands are described, particularly those regarding planar chirality, rigid bulkiness, and ease of derivatization. The role of planar chirality in 1,2- and 1,1'-disubstituted ferrocene systems is discussed. By using a bulky ferrocene fragment, novel ferrocene ligands were designed, and high enantioselectivity and regioselectivity were achieved in the allylic substitution reaction of monosubstituted allyl substrates. Using the tunable electronic properties of a diphosphine-oxazoline ferrocenyl ligand, the regioselectivity of the intermolecular asymmetric Heck reaction was also examined.
PTEN Induces Cell Cycle Arrest by Decreasing the Level and Nuclear Localization of Cyclin D1 (2003)

PTEN is a tumor suppressor frequently inactivated in brain, prostate, and uterine cancers that acts as a phosphatase on phosphatidylinositol-3,4,5-trisphosphate, antagonizing the activity of the phosphatidylinositol 3'-OH kinase. PTEN manifests its tumor suppressor function in most tumor cells by inducing G(1)-phase cell cycle arrest. To study the mechanism of cell cycle arrest, we established a tetracycline-inducible expression system for PTEN in cell lines lacking this gene. Expression of wild-type PTEN but not of mutant forms unable to dephosphorylate phosphoinositides reduced the expression of cyclin D1. Cyclin D1 reduction was accompanied by a marked decrease in endogenous retinoblastoma (Rb) protein phosphorylation on cyclin D/CDK4-specific sites, showing an early negative effect of PTEN on Rb inactivation. PTEN expression also prevented cyclin D1 from localizing to the nucleus during the G(1)- to S-phase cell cycle transition. The PTEN-induced localization defect and the cell growth arrest could be rescued by the expression of a nucleus-persistent mutant form of cyclin D1, indicating that an important effect of PTEN is at the level of nuclear availability of cyclin D1. Constitutively active Akt/PKB kinase counteracted the effect of PTEN on cyclin D1 translocation. The data are consistent with an oncogenesis model in which a lack of PTEN fuels the cell cycle by increasing the nuclear availability of cyclin D1 through the Akt/PKB pathway.
High dynamic range imaging and image-based lighting (2005)

Article Share on High dynamic range imaging and image-based lighting Authors: Erik Reinhard University of Central Florida University of Central FloridaView Profile , Paul Debevec USC ICT USC ICTView Profile , Greg Ward Anyhere Software Anyhere SoftwareView Profile , Sumanta Pattanaik University of Central Florida University of Central FloridaView Profile Authors Info & Claims SIGGRAPH '05: ACM SIGGRAPH 2005 CoursesJuly 2005 Pages 1–eshttps://doi.org/10.1145/1198555.1198707Online:31 July 2005Publication History 7citation796DownloadsMetricsTotal Citations7Total Downloads796Last 12 Months6Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Druck und Gesundheit – aus der Sicht von Psychotherapeut/innen (2011)

In den vergangenen 30 Jahren ist der Krankenstand der gesetzlich Versicherten in Deutschland im langfristigen Trend zurückgegangen. Er lag 1975 in der alten Bundesrepublik bei 5,3 % und 2008 (Ost- und Westdeutschland) bei 3,3 % (vgl. Kocyba & Voswinkel, 2007: 9). Mit 3,7 % hatte das Gesundheitswesen den höchsten Krankenstand, gefolgt von der Öffentlichen Verwaltung mit 3,6 %. Bei „Organisationen, Verbänden, sozialen Einrichtungen“ betrug der Krankenstand 2,9 %, bei Banken/Versicherungen 2,6 % und im IT-Bereich/Datenverarbeitung 2,3 % (vgl. DAK Forschung, 2009: 91).
Listeria-Based Anti-Infective Vaccine Strategies (2006)

Listeria monocytogenes is a Gram-positive intracellular bacterium that has accounted for a significant proportion of human foodborne infections in recent decades. Although infection with sublethal level of L. monocytogenes generates enduring immunity, it is impractical to apply intact virulent strains as vaccine. Through use of killed, attenuated, naturally avirulent, subcellular and DNA vaccine preparations, significant protection has been achieved in experimental animals against listeriosis. Being a robust bacterium capable of eliciting all aspects of cell-mediated immunity, L. monocytogenes has the potential to become an ideal vector for vaccine delivery against other infective agents. Furthermore, with a high safety threshold, L. monocytogenes has increasingly been recognized as a vaccine vector of choice for a range of bacterial, viral and parasitic pathogens. Not surprisingly, a large number of patents have been filed in recent years that exploit attenuated or naturally avirulent L. monocytogenes strains or its listeriolysin (LLO) protein to enhance specific T cell response in the vaccine recipients or to direct vaccine molecule to the cytoplasm of host cells. Following a brief review on the key features of L. monocytogenes infection and host immune responses to listeriosis, this article summarizes the current state and recent progresses in the Listeria-based vaccine strategies that have been developed against bacterial, viral and parasitic diseases, together with a discussion on the future development trends in the utilization of L. monocytogenes as vaccine vector.
Effects of Ambient Air and Temperature on Ionic Gel Gated Single-Walled Carbon Nanotube Thin-Film Transistor and Circuits (2015)

Single-walled carbon nanotube thin-film transistor (SWCNT TFT) and circuits were fabricated by fully inkjet printing gold nanoparticles as source/drain electrodes, semiconducting SWCNT thin films as channel materials, PS-PMMA-PS/EMIM TFSI composite gel as gate dielectrics, and PEDOT/PSS as gate electrodes. The ionic gel gated SWCNT TFT shows reversible conversion from p-type transistor behavior in air to ambipolar features under vacuum due to reversible oxygen doping in semiconducting SWCNT thin films. The threshold voltages of ionic gel gated SWCNT TFT and inverters are largely shifted to the low value (0.5 V for p-region and 1.0 V for n-region) by vacuum annealing at 140 °C to exhausively remove water that is incorporated in the ionic gel as floating gates. The vacuum annealed ionic gel gated SWCNT TFT shows linear temperature dependent transconductances and threshold voltages for both p- and n-regions. The strong temperature dependent transconductances (0.08 μS/K for p-region, 0.4 μS/K for n-region) indicate their potential application in thermal sensors. In the other hand, the weak temperature dependent threshold voltages (-1.5 mV/K for p-region, -1.1 mV/K for n-region) reflect their excellent thermal stability.
Transcriptional repression by the Caenorhabditis elegans germ-line protein PIE-1 (1999)

In the early Caenorhabditis elegans embryo, maternally expressed PIE-1 protein is required in germ-line blastomeres to inhibit somatic differentiation, maintain an absence of mRNA transcription, and block phosphorylation of the RNA polymerase II large subunit (Pol II) carboxy-terminal domain (CTD). We have determined that PIE-1 can function as a transcriptional repressor in cell culture assays. By fusing PIE-1 sequences to the yeast GAL4 DNA-binding domain, we have identified a PIE-1 repression domain that appears to inhibit the transcriptional machinery directly. A sequence element that is required for this repressor activity is similar to the Pol II CTD heptapeptide repeat, suggesting that the PIE-1 repression domain might target a protein complex that can bind the CTD. An alteration of this sequence element that blocks repression also impairs the ability of a transgene to rescue a pie-1 mutation, suggesting that this repressor activity may be important for PIE-1 function in vivo.
Synthesis of Telechelic Polyisoprene via Ring-Opening Metathesis Polymerization in the Presence of Chain Transfer Agent (2010)

Telechelic polyisoprene was synthesized via the ring-opening metathesis polymerization (ROMP) of 1,5-dimethyl-1,5-cyclooctadiene (DMCOD) in the presence of cis-1,4-diacetoxy-2-butene as a chain transfer agent (CTA). This method afforded telechelic polymer in excellent yield, and the acetoxy groups were successfully removed to yield α,ω-hydroxy end-functionalized polyisoprene with potential for subsequent reactions. Efficient, quantitative incorporation of CTA was achieved, and NMR spectroscopy was utilized to confirm the chemical identity of the polymer end groups. Polymerization of discrete DMCOD monomer generated polyisoprene with excellent regioregularity in the polymer backbone. Successful ROMP of sterically challenging DMCOD in the presence of a CTA for chain end-functionalization was borne out through screening of a variety of Ru-based olefin metathesis catalysts.
The First Report of Cyclopolymerization of Bis(oxazolines) To Give Optically Active Polymacrocycles (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe First Report of Cyclopolymerization of Bis(oxazolines) To Give Optically Active PolymacrocyclesRutger D. Puts and Dotsevi Y. SogahCite this: Macromolecules 1995, 28, 1, 390–392Publication Date (Print):January 1, 1995Publication History Published online1 May 2002Published inissue 1 January 1995https://pubs.acs.org/doi/10.1021/ma00105a057https://doi.org/10.1021/ma00105a057research-articleACS PublicationsRequest reuse permissionsArticle Views101Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Pronoun Problem: Possible Changes in the use of Epicene Pronouns (2011)

This study explores the possible bias of gender in the English language and was designed to replicate and extend Noll (1999). We assessed reaction times to lexical decision tasks (i.e., deciding whether a letter string forms a word) following sentences containing supposedly epicene (gender neutral) pronouns "he" and singular "they." We asked whether both feminine and masculine words would be equally accessible following sentences utilizing the epicene "he" and singular "they." Participants ( N=208) were presented with a series of opinion statements and indicated whether they agreed or disagreed. At various points within the sentences, the participants completed the lexical decision task. We measured the amount of time it took to complete the lexical decision task following the pronoun. We found that participants respond faster to male gendered words paired with epicene "he" than female gendered words, also that singular "they" had similar reaction times. However, these results were not statistically significant. Future research should modify the research design, taking into account the limitations of our study.
Micromachines on the march (1994)

Miniature electromechanical sensors and actuators can be mass produced on silicon wafers much like ICs. They combine readily with signal-processing circuitry into powerful tools that measure, analyze, and control their environments. The authors describe the fabrication techniques, surface activity, atomic bonds, LIGA and SLIGA, and bury and destroy. The following applications are discussed: pressure sensors, medical equipment, industrial/consumer equipment, avionics, and mechanical applications.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A Biometric Study of Human Basal Metabolism (1918)

BENEDICTtance less than 8ep from the point P, then X and P lie together in a connected sub-set of M every point of which is at a distance of less than e from the point P. The set M is said to be uniformly connected im kleinen if for every positive number e there exists a positive num- ber 68 such that if P1 and P2 are two points of M at a distance apart less than 58 then they lie together in a connected sub-set of M every point of which is at a distance of less than e from P1. Cf.
A hormone-encoding gene identifies a pathway for cardiac but not skeletal muscle gene transcription. (1994)

In contrast to skeletal muscle, the mechanisms responsible for activation and maintenance of tissue-specific transcription in cardiac muscle remain poorly understood. A family of hormone-encoding genes is expressed in a highly specific manner in cardiac but not skeletal myocytes. This includes the A- and B-type natriuretic peptide (ANP and BNP) genes, which encode peptide hormones with crucial roles in the regulation of blood volume and pressure. Since these genes are markers of cardiac cells, we have used them to probe the mechanisms for cardiac muscle-specific transcription. Cloning and functional analysis of the rat BNP upstream sequences revealed unexpected structural resemblance to erythroid but not to muscle-specific promoters and enhancers, including a requirement for regulatory elements containing GATA motifs. A cDNA clone corresponding to a member of the GATA family of transcription factors was isolated from a cardiomyocyte cDNA library. Transcription of this GATA gene is restricted mostly to the heart and is undetectable in skeletal muscle. Within the heart, GATA transcripts are localized in ANP- and BNP-expressing myocytes, and forced expression of the GATA protein in heterologous cells markedly activates transcription from the natural cardiac muscle-specific ANP and BNP promoters. This GATA-dependent pathway defines the first mechanism for cardiac muscle-specific transcription. Moreover, the present findings reveal striking similarities between the mechanisms controlling gene expression in hematopoietic and cardiac cells and may have important implications for studies of cardiogenesis.
Data remanence in semiconductor devices (2001)

A paper published in 1996 examined the problems involved in truly deleting data from magnetic storage media and also made a mention of the fact that similar problems affect data held in semiconductor memory. This work extends the brief coverage of this area given in the earlier paper by providing the technical background information necessary to understand remanence issues in semiconductor devices. Data remanence problems affect not only obvious areas such as RAM and non-volatile memory cells but can also occur in other areas of the device through hot-carrier effects (which change the characteristics of the semiconductors in the device), electromigration (which physically alter the device itself), and various other effects which are examined alongside the more obvious memory-cell remanence problems. The paper concludes with some design and device usage guidelines which can be useful in reducing remanence effects.
Interaction of ATP Sensor, cAMP Sensor, Ca2+ Sensor, and Voltage-dependent Ca2+ Channel in Insulin Granule Exocytosis (2004)

ATP, cAMP, and Ca(2+) are the major signals in the regulation of insulin granule exocytosis in pancreatic beta cells. The sensors and regulators of these signals have been characterized individually. The ATP-sensitive K(+) channel, acting as the ATP sensor, couples cell metabolism to membrane potential. cAMP-GEFII, acting as a cAMP sensor, mediates cAMP-dependent, protein kinase A-independent exocytosis, which requires interaction with both Piccolo as a Ca(2+) sensor and Rim2 as a Rab3 effector. l-type voltage-dependent Ca(2+) channels (VDCCs) regulate Ca(2+) influx. In the present study, we demonstrate interactions of these molecules. Sulfonylurea receptor 1, a subunit of ATP-sensitive K(+) channels, interacts specifically with cAMP-GEFII through nucleotide-binding fold 1, and the interaction is decreased by a high concentration of cAMP. Localization of cAMP-GEFII overlaps with that of Rim2 in plasma membrane of insulin-secreting MIN6 cells. Localization of Rab3 co-incides with that of Rim2. Rim2 mutant lacking the Rab3 binding region, when overexpressed in MIN6 cells, is localized exclusively in cytoplasm, and impairs cAMP-dependent exocytosis in MIN6 cells. In addition, Rim2 and Piccolo bind directly to the alpha(1)1.2-subunit of VDCC. These results indicate that ATP sensor, cAMP sensor, Ca(2+) sensor, and VDCC interact with each other, which further suggests that ATP, cAMP, and Ca(2+) signals in insulin granule exocytosis are integrated in a specialized domain of pancreatic beta cells to facilitate stimulus-secretion coupling.
A era da mídia exterior (2008)

No mundo urbano em que vivemos, passamos mais tempo fora de casa, que dentro. A Midia Exterior atinge as pessoas ao longo do dia e aumenta o recall das midias tradicionais. O Brasil, com cerca de 80 porcento da populacao nas cidades, tem um enorme potencial a ser explorado. Por que a Midia Exterior nao goza ainda do prestigio e do sucesso devidos? A simplicidade de operacao talvez tenha levado a um quase desleixo com os dados tecnicos. Uma outra deficiencia e a falta de conteudo, mas que tem seu lado positivo. Talvez nao tenhamos todas as respostas, mas vao desde a falta de informacao e pesquisa sobre o meio ate um melhor controle por parte das empresas e pelo poder publico municipal. De qualquer forma, havera sempre espaco para uma luminosa Times Square nas grandes cidades e havera tambem anunciantes prontos a tirar proveito dessa eficiente midia que enfeita e alegra as grandes cidades como Sao Paulo, Los Angeles, Londres e Nova York.
β-Cell-specific inactivation of the mouseIpf1/Pdx1 gene results in loss of the β-cell phenotype and maturity onset diabetes (1998)

To study the late beta-cell-specific function of the homeodomain protein IPF1/PDX1 we have generated mice in which the Ipf1/Pdx1 gene has been disrupted specifically in beta cells. These mice develop diabetes with age, and we show that IPF1/PDX1 is required for maintaining the beta cell identity by positively regulating insulin and islet amyloid polypeptide expression and by repressing glucagon expression. We also provide evidence that IPF1/PDX1 regulates the expression of Glut2 in a dosage-dependent manner suggesting that lowered IPF1/PDX1 activity may contribute to the development of type II diabetes by causing impaired expression of both Glut2 and insulin.