gebhart/patrepeval huggingface dataset:
Linkage Heterogeneity of Autosomal Dominant Polycystic Kidney Disease (1988)

Autosomal dominant polycystic kidney disease has been shown to be closely linked to the alpha-hemoglobin complex on the short arm of chromosome 16. We describe a five-generation kindred, descendants of Sicilian immigrants, in which the disease occurs but without linkage to the alpha-hemoglobin complex. DNA probes were used in genetic-linkage studies on blood samples from 163 family members, of whom 71 were affected by or at risk for autosomal dominant polycystic kidney disease. Diagnoses were confirmed by ultrasound examination. In this family the frequency of recombination between the alpha-hemoglobin complex and the region previously shown to contain the mutation causing polycystic kidney disease exceeded 24 percent, indicating a mutation at a different locus. The clinical findings in this family were indistinguishable from those in other families with polycystic kidney disease. We conclude that there is a second gene for autosomal dominant polycystic kidney disease. This apparent heterogeneity means that prenatal and presymptomatic diagnosis must be approached with caution until a method is found to distinguish between the two forms of the disease.
Serologic microbial associated markers can predict Crohn's disease behaviour years before disease diagnosis (2016)

The presence and accumulation of circulating anti-microbial antibodies years before Crohn's disease diagnosis was associated with complicated Crohn's disease at or shortly after diagnosis.
Induction of Apoptosis of Metastatic Mammary Carcinoma Cells In Vivo by Disruption of Tumor Cell Surface CD44 Function (1997)

To understand how the hyaluronan receptor CD44 regulates tumor metastasis, the murine mammary carcinoma TA3/St, which constitutively expresses cell surface CD44, was transfected with cDNAs encoding soluble isoforms of CD44 and the transfectants (TA3sCD44) were compared with parental cells (transfected with expression vector only) for growth in vivo and in vitro. Local release of soluble CD44 by the transfectants inhibited the ability of endogenous cell surface CD44 to bind and internalize hyaluronan and to mediate TA3 cell invasion of hyaluronan-producing cell monolayers. Mice intravenously injected with parental TA3/St cells developed massive pulmonary metastases within 21-28 d, whereas animals injected with TA3sCD44 cells developed few or no tumors. Tracing of labeled parental and transfectant tumor cells revealed that both cell types initially adhered to pulmonary endothelium and penetrated the interstitial stroma. However, although parental cells were dividing and forming clusters within lung tissue 48 h following injection, >80% of TA3sCD44 cells underwent apoptosis. Although sCD44 transfectants displayed a marked reduction in their ability to internalize and degrade hyaluronan, they elicited abundant local hyaluronan production within invaded lung tissue, comparable to that induced by parental cells. These observations provide direct evidence that cell surface CD44 function promotes tumor cell survival in invaded tissue and that its suppression can induce apoptosis of the invading tumor cells, possibly as a result of impairing their ability to penetrate the host tissue hyaluronan barrier.
The RHD gene is highly detectable in RhD-negative Japanese donors. (1997)

Recent molecular studies on the Rh blood group system have shown that the Rh locus of each haploid RhD-positive chromosome is composed of two structural genes: RHD and RHCE, whereas the locus is made of a single gene (RHCE) on each haploid RhD-negative chromosome. We analyzed the presence or absence of the RHD gene in 130 Japanese RhD-negative donors using the PCR method. The RhD-negative phenotypes consisted of 34 ccEe, 27 ccee, 17 ccEE, 26 Ccee, 19 CcEe, 1 CcEE, and 6 CCee. Among them, 36 (27.7%) donors demonstrated the presence of the RHD gene. Others showed gross or partial deletions of the RHD gene. These results were confirmed by Southern blot analysis. Additionally, the RHD gene detected in the RhD-negative donors seemed to be intact through sequencing of the RhD polypeptide cDNA and the promoter region of RHD gene. The phenotypes of these donors with the RHD gene were CC or Cc, but not cc. It suggested that there is some relationship between the RHD gene and the RhC phenotypes in RhD-negative individuals. In Caucasian RhD-negative individuals, the RHD gene has not been found outside of the report of Hyland et al. (Hyland, C.A., L.C. Wolter, and A. Saul. 1994. Blood. 84:321-324). The discrepant data on the RHD gene in RhD-negative donors between Japanese and Caucasians appear to be derived from the difference of the frequency of RhD-negative and RhC-positive phenotypes. Careful attention is necessary for clinicians in applying RhD genotyping to clinical medicine.
Proactive mobile wireless networks : an infrastructureless wireless network architecture for delay-sensitive applications (2008)

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2008.
Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats (1965)

Abstract In the autoradiograms of young rats injected with thymidine‐H 3 many of the granule cells of the dentate gyrus were found labeled. The number of labeled cells declined rapidly with increased age at the time of injection. Histological studies showed the presence in young rats of a large germinal matrix of mitotic cells in the ependymal and subependymal layers of the third and lateral ventricles. The areal extent and cell population of this germinal pool declined rapidly from birth on, with a transient rise with a peak at about 15 days. During this latter period the number of “undifferentiated” cells near the granular layer of the dentate gyrus showed a rapid rise with a subsequent decline. The decline in the number of “undifferentiated” cells was accompanied by a rise in the number of differentiated granule cells. Cell counts in homologous parts of the dentate gyrus indicated a six‐fold increase in the number of differentiated granule cells from birth to three months. We postulated that undifferentiated cells migrate postnatally from the forebrain ventricles to the hippocampus where they become differentiated. The possible functional significance of delayed hippocampal neurogenesis is discussed with reference to our finding of incorporation of testosterone‐H 3 by cells of the hippocampus, implicating that they may function as receptors of gonadal hormones.
Identification of Phospholipase C-γ1 as a Mitogen-activated Protein Kinase Substrate (2004)

The discovery of sequence motifs that mediate protein-protein interactions, coupled with the availability of protein amino acid sequence data, allows for the identification of putative protein binding pairs. The present studies were based on our identification of an amino acid sequence in phosphatidylinositol-specific phospholipase C-gamma1 (PLC-gamma1) that fits the consensus sequence for a mitogen-activated protein kinase (MAPK) binding site, termed the D-domain. Extracellular signal-regulated kinase 2 (ERK2), an MAPK, and phospho-ERK2 were bound by an immobilized peptide sequence containing the identified PLC-gamma1 D-domain. Furthermore, a peptide containing the PLC-gamma1 D-domain was able to competitively inhibit the in vitro phosphorylation of recombinant PLC-gamma1 by recombinant phospho-ERK2, whereas a control peptide derived from a distant region of PLC-gamma1 was ineffective. Similarly, the peptide containing the PLC-gamma1 D-domain, but not the control peptide, competitively inhibited the in vitro phosphorylation of Elk-1 and c-Jun catalyzed by recombinant phospho-ERK2 and phospho-c-Jun N-terminal kinase 3 (phospho-JNK3), another type of MAPK, respectively. Incubation of anti-PLC-gamma1 immunocomplexes isolated from rat brain with recombinant phospho-ERK2 opposed the increase in PLC-gamma1-catalyzed hydrolysis of phosphatidylinositol 4,5-P(2) (PtdIns(4,5)P(2)), which was produced by a tyrosine kinase associated with the immunocomplexes, whereas in vitro phosphorylation of recombinant PLC-gamma1 by recombinant phospho-ERK2 did not alter PLC-gamma1-catalyzed PtdIns(4,5)P(2) hydrolysis. These studies have uncovered a previously unidentified mechanism for the integration of PLC-gamma1- and ERK2-dependent signaling.
How fast is too fast? evaluating fast forward surrogates for digital video (2003)

To support effective browsing, interfaces to digital video libraries should include video surrogates (i.e., smaller objects that can stand in for the videos in the collection, analogous to abstracts standing in for documents). The current study investigated four variations (i.e., speeds) of one form of video surrogate: a fast forward created by selecting every Nth frame from the full video. In addition, it tested the validity of six measures of user performance when interacting with video surrogates. Forty-five study participants interacted with all four versions of the fast forward surrogate, and completed all six performance tasks with each. Surrogate speed affected performance on four of the measures: object recognition (graphical), action recognition, linguistic gist comprehension (full text), and visual gist comprehension. Based on these results, we recommend a fast forward default speed of 1:64 of the original video keyframes. In addition, users should control the choice of fast forward speed to adjust for content characteristics and personal preferences.
A novel broadband DBR laser for DWDM networks with simplified quasi-digital wavelength selection (2002)

A surface ridge DBR laser has been fabricated using conventional InP processing techniques, with the Bragg gratings defined by e-beam lithography. For a phase grating comb spacing of 6.8 nm, a nine grating device is predicted to yield complete coverage over >45 nm, sufficient to cover C- or L-band.
The Role of Text Analytics and Information Retrieval in the Accounting Domain (2010)

ABSTRACT: Textual documents proliferate throughout accounting practice, and a wide variety of groups make financial decisions based on written guidance. The Generally Accepted Accounting Principles (GAAP), along with annual corporate financial statements and other reporting narratives, are important sources of such guidance and information. This paper examines the literature in two major areas relevant to text analytics and information retrieval in the accounting domain: (1) the manual and computational content analysis of accounting narratives, accounting readability studies, and related text-mining work, and (2) the information retrieval literature stream that addresses the extraction of both text elements as well as quantities imbedded in text from accounting documents, and includes the impact of understanding the accounting lexicon upon retrieval from digital accounting documents. We use the goals in developing the GAAP Codification, as expressed by the Financial Accounting Standards Board (FASB) in their Notice to Constituents (FASB 2009), as a starting point for reviewing the literature. The paper concludes with a map for suggested future research in accounting text analytics and information retrieval.
Promising novel therapeutic approaches in the management of gastrointestinal stromal tumors (2016)

Primary and secondary resistance to currently available licensed tyrosine kinase inhibitors poses a real clinical challenge in the management of advanced gastrointestinal stromal tumors. Within the frame of early phase clinical trials novel systemic treatments are currently being evaluated to target both the well explored and novel emerging downstream effectors of KIT and PDGFRA signaling. Alternative therapeutic approaches also include exploring novel inhibitors of the KIT/PDGFRA receptors, immune checkpoint and cyclin-dependent kinase inhibitors. The final clinical trial outcome data for these agents are highly anticipated. Integration of new diagnostic techniques into routine clinical practice can potentially guide tailored delivery of agents in the treatment of a highly polyclonal, heterogeneous disease such as heavily pretreated advanced gastrointestinal stromal tumor.
High throughput method for prototyping three-dimensional, paper-based microfluidic devices (2012)

This paper describes an efficient and high throughput method for fabricating three-dimensional (3D) paper-based microfluidic devices. The method avoids tedious alignment and assembly steps and eliminates a major bottleneck that has hindered the development of these types of devices. A single researcher now can prepare hundreds of devices within 1 h.
Immunologic responses to Candida albicans. III. Effects of passive transfer of lymphoid cells or serum on murine candidiasis. (1978)

Passive transfer of immune serum gave a significant degree of protection against deep seated candidiasis in mice. Repeated attempts to transfer resistance by the transfer of sensitized lymphoid cells gave negative results, even though cutaneous delayed hypersensitivity was transferred by the cells. The results suggest that cell-mediated immunity is not of primary importance in this model of murine candidiasis, and that humoral immunity contributes to protection.
Current aspects of nasal drug delivery (2006)

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TLR2 and TLR4 signaling shapes specific antibody responses to Salmonella typhi antigens (2009)

TLR directly induce innate immune responses by sensing a variety of microbial components and are critical for the fine-tuning of subsequent adaptive immune responses. However, their impact and mechanism of action on antibody responses against bacterial antigens are not yet fully understood. Salmonella enterica serovar Typhi (S. typhi) porins have been characterized as inducers of long-lasting specific antibody responses in mice. In this report, we show that immunization of TLR4-deficient (TLR4(-/-)), myeloid differentiating gene 88-deficient and Toll/IL-R domain-containing adaptor-inducing IFN-beta-deficient mice with S. typhi porins led to significantly reduced B-cell responses. TLR2(-/-) mice, as well, showed reduced IgG titers with a more pronounced impairment in the production of IgG3 anti-porins antibodies. Adoptive transfer of TLR2(-/-)- or TLR4(-/-)-B cells into B-cell-deficient mice revealed a direct effect of TLR4 on B cells for the primary IgM response, whereas stimulation of B cells via TLR2 was important for IgG production. Furthermore, S. typhi porins were found to efficiently elicit maturation of CD11c(+) conventional DC. Taken together, S. typhi porins represent not only a suitable B-cell antigen for vaccination, but exhibit potent TLR-dependent stimulatory functions on B cells and DC, which help to further enhance and shape the antibody response.
The identification of p130cas-binding proteins and their role in cellular transformation. (1996)

Adaptor proteins play an important role in signal transduction by regulating the establishment and maintenance of functionally important protein complexes. A recently described member of this group of proteins is p130cas (CAS), which contains numerous sequence motifs predicted to be involved in mediating protein-protein interactions. We propose that adaptor molecules like CAS may help determine the response of a cell to a particular signal by interacting with specific subsets of cellular proteins. To test this hypothesis, we have identified potential binding partners of CAS that may play a rote in cellular transformation by the oncoproteins v-SRC and/or v-CRK. We show that individual domains of CAS associate with specific subsets of proteins in vitro, and that many of these interactions are dependent on the state of tyrosine-phosphorylation of CAS. Sequences necessary for interacting with the focal adhesion kinase pp125FAK (FAK), v-SRC and v-CRK have been mapped to distinct regions of CAS. In addition, the identification of a number of putative CAS-binding partners that are present in crk-transformed cell extracts but undetectable in normal and src-transformed cell extracts supports a model in which unique protein complexes are formed in response to different signals.
Broadband Raman amplifiers in the spectral range of 1480-1620 nm (2003)

Raman amplifiers using germano-silica fibers are demonstrated with a continuous operation bandwidth from 20 nm to more than 100 nm, 20-40 dB net gain and 4.5-7 dB noise figure. Single-, dual-, and triple-wavelength pumping are implemented in the configuration incorporating circulators for pump/signal multiplexing.
FILLING PREPOSITION-BASED TEMPLATES TO CAPTURE INFORMATION FROM MEDICAL ABSTRACTS (2001)

Due to the recent explosion of information in the biomedical field, it is hard for a single researcher to review the complex network involving genes, proteins, and interactions. We are currently building GeneScene, a toolkit that will assist researchers in reviewing existing literature, and report on the first phase in our development effort: extracting the relevant information from medical abstracts. We are developing a medical parser that extracts information, fills basic prepositional-based templates, and combines the templates to capture the underlying sentence logic. We tested our parser on 50 unseen abstracts and found that it extracted 246 templates with a precision of 70%. In comparison with many other techniques, more information was extracted without sacrificing precision. Future improvement in precision will be achieved by correcting three categories of errors.
Effects on Cells (1996)

In most cases, the disturbances of bodily function that are manifested as the signs and symptoms of viral disease result from the direct effects of viruses on cells. Knowledge of the morphologic, physiologic, biochemical, and immunologic effects of viruses on cells is essential in understanding the pathophysiology of viral disease and in developing accurate diagnostic procedures and effective treatment.Virus-host cell interactions (Table 44-1) may produce either 1) cytocidal (cytolytic) infections, in which production of new infectious virus kills the cell; 2) persistent infections, in which the virus or its genome resides in some or all of the cells without killing most of them; or 3) transformation, in which the virus does not kill the cell, but produces genetic, biochemical, physiologic, and morphologic changes that may result in the acquisition of malignant properties (see also Ch. 47). The type of virus infection and the virus-induced effects on cells are dependent on the virus, the cell type and species, and often the physiologic state of the cell.
Immunoglobulin D-deficient mice can mount normal immune responses to thymus-independent and -dependent antigens. (1993)

To examine the in vivo function of IgD we generated mice deficient for IgD by gene targeting. The IgD-mice show a reduced B-cell compartment with 30-50% less B cells in the spleen and lymph nodes but show a normal pre-B-cell compartment. The surface-IgD- B cells express two to three times more surface IgM than B cells of control animals. Serum concentrations of the immunoglobulin isotypes of IgD- mice are almost normal, indicating that surface-IgD expression is not necessary for class switching of B cells. Immunization experiments showed that IgD- mice could respond well to thymus-dependent and -independent antigens. After immunization normal germinal centers developed in the IgD- mice. These data suggest that IgD is not necessary for the induction of immune responses but may be important in homeostasis of cells in the B-cell compartment.
All Good Things Must Come to an End: How Is Notch Signaling Turned off? (1999)

Turning off signaling pathways can be just as important for proper biological regulation as turning them on. The Notch signaling pathway controls development of the nervous system in Drosophila. Proteolysis of Notch appears to initiate signaling, but further proteolysis may also terminate signals from this pathway. Kopan discusses mechanisms that limit signaling by Notch, including recent evidence that degradation of specifically targeted proteins by the proteasome is required.
Oxford Textbook of Medicine. (1988)

The <italic>Oxford Textbook of Medicine</italic> is published online and has been regularly updated for many years, but the production of a new and very substantially updated edition provides a moment when it is natural and proper to reflect on what has changed in Medicine—and what has not—in recent years. The sixth edition of the textbook considers exactly what modern medicine has to offer patients and their doctors. Advances in biomedical sciences, the broader context of health and disease, patients and their expectations, and access to medical knowledge are some of the key areas examined in detail among the 30 sections of the book. Sections include introductory sections on patients and their treatment, background to medicine, cell biology, and immunological mechanisms, as well as more subject-specific sections such as infectious diseases, neurological disorders, cardiovascular disorders, rheumatology, and the kidney, as well as many more.
Built-Up Films of Barium Stearate and Their Optical Properties (1937)

The technique of depositing successive single layers of molecules of various stearates on a solid surface is described. Films containing 3001 layers have been built of barium-copper stearate. A photograph shows films built in a series of steps having intervals of 2 molecular layers. The contrast of the steps is plainly visible when the slide is illuminated by polarized light at angles near grazing incidence. By measuring the angles at which films containing known numbers of layers reflect minimum intensity of monochromatic light for the first five interference fringes, the thickness per layer and refractive index can be calculated with great accuracy. The thickness per layer of barium stearate was found to be 24.40A. The presence of traces of foreign substances in the water affect the spacing by 1 to 3 percent. The films are uniaxial crystals, the optic axis being perpendicular to the surface on which the films are built. The refractive index of the ordinary ray, ${n}_{1}$, and of the extraordinary ray in a direction perpendicular to the axis, ${n}_{3}$, are ${n}_{1}=1.491$, ${n}_{3}=1.551$. Equations are given which describe the refraction of the extraordinary ray, the intensity of the rays reflected from the upper surface and from the film solid boundary, the phase change at the boundaries, Brewster's angle, and other special properties of birefringent films. The intensity of the light and dark fringes reflected by films built on a series of glasses of known refractive indices is used as a measure of ${n}_{1}$. Skeleton films. Barium stearate films built at $\mathrm{pH}&lt;7.0$ are composed of a mixture of stearic acid and neutral stearate. The stearic acid can be dissolved by benzene leaving a skeleton of stearate which is birefringent and has refractive indices much lower than those of the normal film. Measurements are given of a skeleton for which ${n}_{1}=1.30$, others for which ${n}_{1}=1.32$, ${n}_{3}=1.39$. Skeletons have been built having ${n}_{1}=1.25$ and 1.22. The skeleton for which ${n}_{1}=1.30$ had 99.2 percent of the thickness of the original film, although only 63.7 percent of its density.