gebhart/patrepeval huggingface dataset:
OBSERVACIONES A LA LEY GENERAL FORESTAL: UNA MIRADA DESDE LA ACADEMIA (2006)

En este contexto y a proposito de la politica ambiental2, dos elementos permiten establecer un marco de partida: de un lado la presencia de los modelos abstractos y su relacion con el funcionamiento de la practica, para lo cual, como senala Gomez (2006) respecto a la advertencia de Hahn (1989) sobre los riesgos de convertir las conclusiones del analisis economico en recomendaciones de caracter practico sin atender al cumulo de circunstancias propias del contexto en que estas recomendaciones se deben aplicar. De todos es sabido que lo que funciona en el mundo de los modelos abstractos no tiene porque necesariamente funcionar en la practica. Esto se debe en parte a que tales modelos sacrifican el realismo en beneficio de la elegancia y la concrecion; por necesidad, los modelos constituyen una simplificacion de las restricciones y el marco institucional en que se deben aplicar las recomendaciones que de ellos se derivan. Tambien es importante tener presente que, en la mayoria de los casos, los problemas que deben resolverse en la practica no son los que pueden tratarse de un modo coherente en un solo modelo de analisis. (Gomez, 2006). Mucho de esto ha ocurrido y seguira probablemente ocurriendo en el caso de las alternativas que se ponen en practica para resolver los problemas ambientales.
Urea-monocalcium phosphate, a component of mixed fertilizers (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUrea-monocalcium phosphate, a component of mixed fertilizersA. William. Frazier, James R. Lehr, and James Pershing. SmithCite this: J. Agric. Food Chem. 1967, 15, 2, 345–347Publication Date (Print):March 1, 1967Publication History Published online1 May 2002Published inissue 1 March 1967https://pubs.acs.org/doi/10.1021/jf60150a025https://doi.org/10.1021/jf60150a025research-articleACS PublicationsRequest reuse permissionsArticle Views206Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Automatic Seizure Detection Based on Nonlinear Dynamical Analysis of EEG Signals and Mutual Information (2018)

The results showed that the nonlinear dynamical analysis based on Rcurrence Quantification Analysis (RQA) can be employed as a suitable approach for characterizing the nonlinear EEG dynamics and detecting the seizure.
Karyoplasmic interaction selection strategy: a general strategy to detect protein-protein interactions in mammalian cells. (1992)

We describe a strategy and reagents for study of protein-protein interactions in mammalian cells, termed the karyoplasmic interaction selection strategy (KISS). With this strategy, specific protein-protein interactions are identified by reconstitution of the functional activity of the yeast transcriptional activator GAL4 and the resultant transcription of a GAL4-regulated reporter gene. Reconstitution of GAL4 function results from specific interaction between two chimeric proteins: one contains the DNA-binding domain of GAL4; the other contains a transcriptional activation domain. Transcription of the reporter gene occurs if the two chimeric proteins can form a complex that reconstitutes the DNA-binding and transcriptional activation functions of GAL4. Using the KISS system, we demonstrate specific interactions for sequences from three different pairs of proteins that complex in the cytoplasm. In addition, we demonstrate that reporter genes encoding cell surface or drug-resistance markers can be specifically activated as a result of protein-protein interactions. With these selectable markers, the KISS system can be used to screen specialized cDNA libraries to identify novel protein interactions.
Quantitative analysis of the immunopotency of genetically transfected dendritic cells (1998)

Dendritic cells (DCs) instruct and activate a naive immune system to mount a response toward foreign proteins. Therefore, it has been hypothesized that an ideal vaccine strategy would be to directly introduce genes encoding antigens into DCs. To test this strategy quantitatively, we have compared the immune response elicited by a genetically transfected DC line to that induced by a fibroblast line, or standard genetic immunization. We observe that a single injection of 500-1,000 transfected DCs can produce a response comparable to that of standard genetic immunization, whereas fibroblasts, with up to 50-fold greater transfection efficiency, were less potent. We conclude that transfection of a small number of DCs is sufficient to initiate a wide variety of immune responses. These results indicate that targeting genes to DCs will be important for controlling and augmenting the immunological outcome in genetic immunization.
Assays for Total Protein (1995)

Abstract This unit describes three copper‐based assays to quantitate total protein: the biuret method, a variation of the Lowry method (Hartree‐Lowry method), and the bicinchinonic acid (BCA) assay. Acid hydrolysis of a protein is coupled with ninhydrin detection to quantitate amino acid content of a sample. Ultraviolet spectrophotometry is used to measure total protein and evaluate samples for the presence of contaminants. The Coomassie dye binding, or Bradford, assay is a quite simple assay and frequently is quite sensitive, although it sometimes gives a variable response depending on how well or how poorly the protein binds the dye in acid pH. Finally, dry weight measurement is used to quantitate pure protein. Support protocols describe a technique for heat sealing glass tubes for acid hydrolysis, sample dialysis in polyacrylamide gel wells to remove low‐molecular‐weight contaminants, and TCA precipitation to precipitate and concentrate proteins and remove low‐molecular‐weight contaminants. In performing total protein assays, there are five issues of concern: (1) sensitivity and technique of the method, (2) clear definition of units, (3) interfering compounds, (4) removal of interfering substances before assaying samples, and (5) correlation of information from various techniques. These concerns are discussed at length in the Commentary of this unit.
OCTOPUS: improving topology prediction by two-track ANN-based preference scores and an extended topological grammar (2008)

OCTOPUS is available as a web server at http://octopus.cbr.su.se.
A Three‐Dimensional Display for Cardiac Activation Mapping (1991)

A three-dimensional display is described that allows activation sequences from the epicardium and endocardium to be shown simultaneously on the same image. Three electrode arrays (epicardial sock, left ventricular balloon, right ventricular balloon) are represented in a three-dimensional perspective by an array of dots that are intensified when activated. This arrangement requires fewer calculations and is easier to interpret than sliced-isochronal maps but cannot represent a complete heart cycle in one image. The three-dimensional display eliminates the distortion caused by two-dimensional diagrams and facilitates activation correlation between electrode arrays. A standard, low cost microcomputer has been used to implement the activation display.
Extremely High Selective Etching of Porous Si for Single Etch-Stop Bond-and-Etch-Back Silicon-on-Insulator (1995)

The etching characteristics of porous Si in comparison with bulk Si have been investigated for the ultra thin film (UTF) single etch-stop bond-and-etch-back silicon-on-insulator (BESOI). It is found out that porous Si can be selectively etched exclusively by a mixture of HF, H 2 O 2 and H 2 O due to the structure-sensitive mechanism, that is, inner reaction by capillary-induced penetration of the etchant into the pores followed by collapsing the Si columns. This extremely high etching selectivity reaches as large as 10 5 , which results in excellent SOI layer thickness variation of less than 7% across 5-inch SOI wafers with sub-µ m and sub-100-nm thicknesses.
High Magnetic Field Study of Chromium Trimer Complexes below 1 K (1988)

Magnetizations of two trinuclear chromium salts are measured in pulsed magnetic fields up to 350 kOe. The experiment is done below 1 K using a 3 He cryostat arranged in a large-bore magnet of 6 cm in diameter. In Cr-propionate, the ground state crossover from S =1/2 to S =3/2 is observed at 272 kOe as a stepwise increase in the magnetization curve. The measurement in Cr-acetate at 0.6 K shows that the increase consists of two steps at 276 kOe and 314 kOe corresponding to two inequivalent sites of magnetic trimers in this compound. The magnitudes of the intra- and inter-trimer exchange interactions are discussed.
Copy number variation detection in whole-genome sequencing data using the Bayesian information criterion (2011)

DNA copy number variations (CNVs) play an important role in the pathogenesis and progression of cancer and confer susceptibility to a variety of human disorders. Array comparative genomic hybridization has been used widely to identify CNVs genome wide, but the next-generation sequencing technology provides an opportunity to characterize CNVs genome wide with unprecedented resolution. In this study, we developed an algorithm to detect CNVs from whole-genome sequencing data and applied it to a newly sequenced glioblastoma genome with a matched control. This read-depth algorithm, called BIC-seq, can accurately and efficiently identify CNVs via minimizing the Bayesian information criterion. Using BIC-seq, we identified hundreds of CNVs as small as 40 bp in the cancer genome sequenced at 10× coverage, whereas we could only detect large CNVs (> 15 kb) in the array comparative genomic hybridization profiles for the same genome. Eighty percent (14/16) of the small variants tested (110 bp to 14 kb) were experimentally validated by quantitative PCR, demonstrating high sensitivity and true positive rate of the algorithm. We also extended the algorithm to detect recurrent CNVs in multiple samples as well as deriving error bars for breakpoints using a Gibbs sampling approach. We propose this statistical approach as a principled yet practical and efficient method to estimate CNVs in whole-genome sequencing data.
IL-2 enhances the function of recombinant poxvirus-based vaccines in the treatment of established pulmonary metastases. (1995)

Neoplastic cells are generally poor immunogens. Transfection of the murine tumor CT-26 with beta-galactosidase (beta-gal), a protein from Escherichia coli, did not alter its growth rate in vivo, or its lethality, and did not elicit a measurable anti-beta-gal immune response. Immunization with beta-gal-expressing recombinant vaccinia viruses (rVV) elicited specific anti-beta-gal cytolytic T lymphocytes, but rVV-beta-gal was only marginally therapeutic when given to tumor-bearing mice. With the aim of expanding the immune response against beta-gal, used here as a model tumor Ag, we gave mice exogenous IL-2 starting 12 h after the poxvirus. The therapeutic effectiveness of the combination of poxvirus and IL-2 was far greater than either of these treatments alone. When the cDNA for IL-2 was inserted into the viral genome of the rVV construct to make a double recombinant (drVV), antitumor activity was further augmented. One mechanism of action may be the enhanced activation or expansion of cytotoxic T cells, because a marked increase in primary cytotoxic responses against vaccinia determinants was observed. Interestingly, other cytokines (mGM-CSF, mTNF-alpha, and mIFN-gamma) inserted into the rVV genome did not modify the efficacy of the rVV constructs. The increase in specific CTL responses against beta-gal by drVV expressing the tumor-associated Ags (TAA) and IL-2 was more pronounced in mice bearing the lacZ-transduced tumor than in those bearing the parental cell line, suggesting that the TAA presented by growing tumor cells can either pre-activate or otherwise amplify the immune response induced by the rVV. Unfortunately, in several long-term surviving mice, tumor recurred that no longer expressed beta-gal. These results indicate that treatment of disseminated tumors by using recombinant viruses expressing TAA can be enhanced by IL-2 provided exogenously, or encoded within the recombinant virus.
Impact of lens aberrations on optical lithography (1997)

All optical projection systems for microlithography depart from perfection because of various lens aberrations, especially when large image field size is combined with high numerical aperture (NA). Such aberrations have a variety of effects on lithographic imaging: shifts in the image position, image asymmetry, reduction of the process window, and the appearance of undesirable imaging artifacts. These undesirable effects are sometimes exacerbated through use of resolution enhancement techniques such as phase-shift masks or nonstandard illumination. This paper examines the impact of different types of aberrations on lithographic imagery through simulation. New techniques for measuring aberrations by examining lithographically printed resist patterns are considered.
Endothelin, a potent peptide agonist in the liver. (1990)

Endothelin, a peptide mediator produced by vascular endothelial cells, caused sustained vasoconstriction of the portal vasculature in the perfused rat liver. The vasoactive effect of endothelin was accompanied by increased glycogenolysis and alterations in hepatic oxygen consumption. The endothelin-induced increase in the portal pressure was concentration-dependent with an EC50 of 1 nM. Endothelin-induced hepatic glycogenolysis was dose-dependent but exhibited a different EC50 than for the vasoconstrictive effects of endothelin. Hepatic vasoconstriction and glycogenolysis following endothelin infusion were inhibited when Ca2+ was removed from the perfusion medium. The endothelin-induced responses in the liver were not altered by prior infusion of phenylephrine (alpha-adrenergic agonist), isoproterenol (beta-adrenergic agonist), angiotensin II, glucagon, platelet-activating factor, or the platelet-activating factor antagonist, BN52021. However, repeated infusion of endothelin resulted in desensitization of the glycogenolytic response but was without a significant effect on hepatic vasoconstriction. Endothelin also stimulated metabolism of inositol phospholipids in isolated hepatocytes and Kupffer cells in primary culture. The present experiments demonstrate, for the first time, that endothelin is a very potent agonist in the liver eliciting both a sustained vasoconstriction of the hepatic vasculature and a significant increase in hepatic glucose output.
The increased prevalence of allergy and the hygiene hypothesis: missing immune deviation, reduced immune suppression, or both? (2004)

Allergic atopic disorders, such as rhinitis, asthma, and atopic dermatitis, are the result of a systemic inflammatory reaction triggered by type 2 T helper (Th2) cell-mediated immune responses against 'innocuous' antigens (allergens) of complex genetic and environmental origin. A number of epidemiological studies have suggested that the increase in the prevalence of allergic disorders that has occurred over the past few decades is attributable to a reduced microbial burden during childhood, as a consequence of Westernized lifestyle (the 'hygiene hypothesis'). However, the mechanisms by which the reduced exposure of children to pathogenic and nonpathogenic microbes results in enhanced responses of Th2 cells are still controversial. The initial interpretation proposed a missing immune deviation of allergen-specific responses from a Th2 to a type 1 Th (Th1) profile, as a result of the reduced production of interleukin-12 and interferons by natural immunity cells which are stimulated by bacterial products via their Toll-like receptors. More recently, the role of reduced activity of T regulatory cells has been emphasized. The epidemiological findings and the experimental evidence available so far suggest that both mechanisms may be involved. A better understanding of this question is important not only from a theoretical point of view, but also because of its therapeutic implications.
Characterization of human tactile pattern recognition performance at different ages (2010)

This study examined tactile pattern recognition performance in human observers (N = 44) in the context of a letter recognition task at the fingertip. Participants were recruited from three different age groups (youth, n = 17; young adults, n = 14; seniors, n = 13) to examine age-related differences in performance. The influence of gender (males vs females) and hand (right vs left) was also examined. Performance was characterized in terms of both response accuracy and associated response times (RTs). Patterns of confusion between letters were also examined. Results showed that age was the most important factor in determining the capacity of our participants to perform fast and accurate pattern recognition. In this respect, younger participants (i.e., youth and young adults) clearly outperformed seniors by showing not only better accuracy and less confusion but also 2-3 times faster RT. By comparison, the combined influence of "hand" and "gender" on recognition performance was only marginal. These results indicate that the ability to perform complex tactile pattern recognition is already well established in youth 10-14 years of age with only minor refinements occurring later in early adulthood. With advancing age, such ability becomes far less efficient, as judged by the drastic increase in RT observed in seniors, in spite of a relatively good accuracy. This suggests that alterations not only at the peripheral receptor level but also at the central processing level might play an important role in limiting the ability of seniors to perform fast and efficient pattern recognition at the fingertip.
Pancreatic Extracts in the Treatment of Diabetes Mellitus. (1922)

Since the year 1889, when von Mering and Minkowski produced severe and fatal diabetes by total removal of the pancreas in dogs, many investigators have endeavoured to obtain some beneficial effect in diabetes mellitus, either by feeding pancreas, or by administration of pancreatic extracts.
Tumor necrosis factor and interferon-gamma induce distinct patterns of endothelial activation and associated leukocyte accumulation in skin of Papio anubis. (1989)

Recombinant human interferon (IFN)-gamma (2 X 10(4) or 2 X 10(5) U), tumor necrosis factor (TNF, 10(4) or 10(5) U), or both were injected intracutaneously into baboons (Papio anubis), and biopsies were examined at various intervals for evidence of altered endothelial cell antigen expression, endothelial morphology, and leukocyte infiltration. IFN-gamma induced increased binding of anti-HLA-DP mAb by 24 hours and a mild-to-moderate accumulation of mononuclear cells. TNF induced increased binding of anti-endothelial leukocyte adhesion molecule (ELAM)-1 mAb by 2 hours that was associated with polymorphonuclear leukocyte accumulation, and increased binding of anti-intercellular adhesion molecule (ICAM)-1 mAb by 9 hours that was associated with the onset of progressive mononuclear leukocyte accumulation. TNF also caused endothelial cell hypertrophy and increased vascular permeability. The combination of IFN-gamma and TNF induced a set of changes that qualitatively resemble those of a delayed hypersensitivity reaction to simian agent 8 envelope antigen. These findings are consistent with the concept that cytokine-activated endothelium plays an important role in the adhesion and subsequent extravasation of leukocytes during immune inflammation.
Effects of steady-state versus stochastic exercise on subsequent cycling performance (1997)

The aims of this investigation were to evaluate the physiological responses to laboratory based stochastic exercise and to assess the effects of stochastic versus steady-state exercise on subsequent cycling time trial (TT) performance. Six competitive cyclists (peak power output (PPO) 432 +/- 39 W (values are mean +/- SD) undertook in a random order two 150-min paced rides that were either constant load (58% of PPO) or stochastic in nature (58 +/- 12.2% of PPO). These rides were immediately followed by a 20-km TT performance on an air-braked ergometer. Mean heart rate (HR) responses throughout the 150-min paced rides and during the subsequent TT were not significantly different between trials. Yet, despite the similarities in HR, the mean time for the TT was significantly faster (26:32 +/- 1:30 vs 28:08 +/- 1:47 min, P < 0.05) and the mean power output was significantly greater (340.3 +/- 44.2 vs 302.5 +/- 42.3 W; 77.8 +/- 10.2 vs 70.0 +/- 9.8% of PPO, P < 0.05) following the steady-state ride. These results demonstrate that following 150 min of steady-state riding, subsequent 20 km TT performance was significantly improved when compared with 150 min of stochastic exercise.
Resistance Analysis of the Hepatitis C Virus NS5A Inhibitor BMS-790052 in an In Vitro Replicon System (2010)

BMS-790052 is the most potent hepatitis C virus (HCV) inhibitor reported to date, with 50% effective concentrations (EC(50)s) of < or = 50 pM against genotype 1 replicons. This exceptional potency translated to rapid viral load declines in a phase I clinical study. By targeting NS5A, BMS-790052 is distinct from most HCV inhibitors in clinical evaluation. As an initial step toward correlating in vitro and in vivo resistances, multiple cell lines and selective pressures were used to identify BMS-790052-resistant variants in genotype 1 replicons. Similarities and differences were observed between genotypes 1a and 1b. For genotype 1b, L31F/V, P32L, and Y93H/N were identified as primary resistance mutations. L23F, R30Q, and P58S acted as secondary resistance substitutions, enhancing the resistance of primary mutations but themselves not conferring resistance. For genotype 1a, more sites of resistance were identified, and substitutions at these sites (M28T, Q30E/H/R, L31M/V, P32L, and Y93C/H/N) conferred higher levels of resistance. For both subtypes, combining two resistance mutations markedly decreased inhibitor susceptibility. Selection studies with a 1b/1a hybrid replicon highlighted the importance of the NS5A N-terminal region in determining genotype-specific inhibitor responses. As single mutations, Q30E and Y93N in genotype 1a conferred the highest levels of resistance. For genotype 1b, BMS-790052 retained subnanomolar potency against all variants with single amino acid substitutions, suggesting that multiple mutations will likely be required for significant in vivo resistance in this genetic background. Importantly, BMS-790052-resistant variants remained fully sensitive to alpha interferon and small-molecule inhibitors of HCV protease and polymerase.