gebhart/patrepeval huggingface dataset:
Radioresistance in head and neck squamous cell carcinoma: Biological bases and therapeutic implications (2014)

Head and neck squamous cell carcinoma (HNSCC) is strongly associated with alcohol and tobacco consumption. Lately, the incidence of human papillomavirus (HPV)-related tumors has shown a significant increase, and HPV-related tumors show distinctive features if compared with the HPV-negative counterpart. Locally advanced HNSCC can be treated with concomitant chemoradiotherapy, but early recurrences sometimes occur. Relapses are often related to an intrinsic radioresistance of the tumors. Alterations in intracellular pathways, primarily involved in cell proliferation, apoptosis, and DNA repair, can lead to radioresistance. Preclinical and clinical evidence highlighted that 3 main pathways, including the epidermal growth factor receptor (EGFR), the phosphotidylinositol-3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR), and the p53 signaling cascades, play a crucial role in radioresistance development. A future approach may consist in the association of radiotherapy (RT) and selective inhibition of the key pathways involved in radioresistance. Phase I, II, and III clinical trials are currently testing these novel treatment strategies.
Radio Interface Evolution Towards 5G and Enhanced Local Area Communications (2014)

The exponential growth of mobile data in macronetworks has driven the evolution of communications systems toward spectrally efficient, energy efficient, and fast local area communications. It is a well-known fact that the best way to increase capacity in a unit area is to introduce smaller cells. Local area communications are currently mainly driven by the IEEE 802.11 WLAN family being cheap and energy efficient with a low number of users per access point. For the future high user density scenarios, following the 802.11 HEW study group, the 802.11ax project has been initiated to improve the WLAN system performance. The 3GPP LTE-advanced (LTE-A) also includes new methods for pico and femto cell's interference management functionalities for small cell communications. The main problem with LTE-A is, however, that the physical layer numerology is still optimized for macrocells and not for local area communications. Furthermore, the overall complexity and the overheads of the control plane and reference symbols are too large for spectrally and energy efficient local area communications. In this paper, we provide first an overview of WLAN 802.11ac and LTE/LTE-A, discuss the pros and cons of both technology areas, and then derive a new flexible TDD-based radio interface parametrization for 5G local area communications combining the best practices of both WiFi and LTE-A technologies. We justify the system design based on local area propagation characteristics and expected traffic distributions and derive targets for future local area concepts. We concentrate on initial physical layer design and discuss how it maps to higher layer improvements. This paper shows that the new design can significantly reduce the latency of the system, and offer increased sleeping opportunities on both base station and user equipment sides leading to enhanced power savings. In addition, through careful design of the control overhead, we are able to improve the channel utilization when compared with LTE-A.
Esophageal combined carcinomas: Immunohoistochemical and molecular genetic studies (2012)

Primary esophageal combined carcinoma is very rare. The authors herein report 2 cases. Case 1 was a combined squamous cell carcinoma and small cell carcinoma, and case 2 was a combined squamous cell carcinoma, adenocarcinoma, and small cell carcinoma. Case 1 was a 67-year-old man with complaints of dysphagia. Endoscopic examination revealed an ulcerated tumor in the middle esophagus, and 6 biopsies were obtained. All 6 biopsies revealed a mixture of squamous cell carcinoma and small cell carcinoma. Both elements were positive for cytokeratin, epithelial membrane antigen, and p53 protein, and had high Ki-67 labeling. The small cell carcinoma element was positive for synaptophysin, CD56, KIT, and platelet-derived growth factor-α (PDGFRA), while the squamous cell carcinoma element was not. Genetically, no mutations of KIT and PDGFRA were recognized. The patient died of systemic carcinomatosis 15 mo after presentation. Case 2 was a 74-year-old man presenting with dysplasia. Endoscopy revealed a polypoid tumor in the distal esophagus. Seven biopsies were taken, and 6 showed a mixture of squamous cell carcinoma, small cell carcinoma, and adenocarcinoma. The 3 elements were positive for cytokeratins, epithelial membrane antigen, and p53 protein, and had high Ki-67 labeling. The adenocarcinoma element was positive for mucins. The small cell carcinoma element was positive for CD56, synaptophysin, KIT, and PDGFRA, but the other elements were not. Mutations of KIT and PDGFRA were not recognized. The patient died of systemic carcinomatosis 7 mo after presentation. These combined carcinomas may arise from enterochromaffin cells or totipotential stem cell in the esophagus or transdifferentiation of one element to another. A review of the literature was performed.
The effects of specific metabolic inhibitors on the energy metabolism of Globodera rostochiensis and Panagrellus redivivus (1989)

Trois indicateurs de l'activite metabolique (consommation d'oxygene, teneur en ATP, degagement de chaleur) sont utilises pour etudier l'action d'inhibiteurs metaboliques specifiques sur les juveniles de 2eme stade de G. r. et les adultes de P. r.. Les hydrates de carbone, lipides et acides amines peuvent etre utilises comme substrat. Un cycle glyoxylate est present. Le composant aerobie est preponderant, avec filieres anaerobies de remplacement. Le degagement de chaleur est le meilleur indicateur et est non destructif.
What to see before you see a movie: Investigating film and television classification (1994)

Review(s) of: What to see before you see a movie: Investigating film and television classification.
Human ERG is a proto-oncogene with mitogenic and transforming activity. (1995)

The ETS related gene, ERG, is one of 20 or more genes belonging to the ETS family of transcription factors. Translocation of the ERG gene t(21;22) results in the chimeric fusion transcript seen in approximately 10% of Ewings sarcomas. In addition, recent studies have shown that a reciprocal translocation t(21;16) of ERG gives rise to two aberrant transcripts seen in some forms of acute myeloid leukaemia. In vitro studies have linked the up regulation of ERG expression with stromal cell independence in erythroleukemic clones and shown that the ERG related genes ETS1 and ETS2 have a mitogenic and transforming activity when overexpressed in NIH3T3 cells. Interestingly ERGB/FLI-1, which is also involved in Ewings sarcoma translocations and shares a very high sequence identify with ERG has been reported to be unable to transform NIH3T3 cells. In this study we investigate the effects of overexpression of ERG on cell proliferation, factor dependence, growth in soft agar and tumorigenesis in nude mice. An ERG expression construct with the human ERG2 cDNA driven by the sheep metallothionein la promoter (sMTERG) was transfected into NIH3T3 cells. Clonal cell lines overexpressing ERG were established. The cell lines became morphologically altered, grew in low serum and serum free media and gave rise to colonies in soft agar suspension. Furthermore, we demonstrate that after subcutaneous injection these clones grow as solid tumors in nude mice. These data demonstrate that c-ERG is a proto-oncogene capable of transforming NIH3T3 cells. Therefore, overexpression or inappropriate expression of ERG may contribute to oncogenesis.
Salivary α-amylase levels as a biomarker of experienced fear (2010)

We recently reported data related to emotions collected in conjunction with a museum exhibit on emotion (Goose Bumps!-The Science of Fear).1 In this addendum, we present additional data collected as part of that study. We collected two commonly measured indices of emotional arousal, salivary cortisol and α-amylase, before and after participants had gone through a realistic fear challenge course as part of the exhibit. We found that α-amylase, but not cortisol, showed a highly specific increase only for those participants who endorsed both emotional arousal and negative valence. By contrast, the fear-inducing course resulted in high arousal but positive valence in some participants; in these, no increased α-amylase was measured. We conclude that salivary α-amylase is a promising biomarker for fearful experiences, and suggest that it is important to pay attention to positively valenced arousal that may be induced by fearful stimuli in a laboratory setting.
Synthesis of 4-Aza-5,6-dimethylbenzimidazole and Biosynthetic Preparation of 4-and 7-Aza-5,6-dimethylbenzimidazolylcobamide (1997)

We report on the preparation of 4-aza-5,6-dimethylbenzimidazolylcobamide and 5,6-dimethyl-7-azabenzimidazolylcobamide. These vitamin B12-analogs were required as reference compounds for comparison with a corrinoid previously isolated in small amounts for Eubacterium limosum grown in the presence of 4(5)-aminoimidazole. 4(7)-Aza-5,6-dimethylbenzimidazole was synthesized from N-1-benzyl-4-nitroimidazole which was reduced to N-1-benzyl-4-aminoimidazole and condensed with 1-dimethylamino-2-methylbutan-3-one to yield N-1-benzyl-4-aza-5,6-dimethylbenzimidazole. The benzyl group of this compound was split off by catalytic hydrogenation to form 4(7)-aza-5,6-dimethylbenzimidazole. 4(7)-Aza-5,6-dimethylbenzimidazole was transformed by a growing culture of Propionibacterium shermanii into 4-aza-5,6-dimethylbenzimidazolylcobamide and 5,6-dimethyl-7-azabenzimidazolylcobamide. Both vitamin B12-analogs were almost as active as Vitamin B12 in a growth test with the vitamin B12-dependent Escherichia coli-mutant DSM 4261.
Enhanced Detection of Oligonucleotides in UV MALDI MS Using the Tetraamine Spermine as a Matrix Additive (1999)

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) has been used to analyze oligonucleotides. However, success has been limited by cation adduction and high detection limits. Both of these problems are due to the high net negative charge that oligonucleotides carry on the phosphodiester backbone. Comatrixes such as ammonium salts with UV absorbers such as 3-hydroxypicolinic acid, 2,4,6-trihydroxyacetophenone, and 6-aza-2-thiothymine have been used to improve the spectral quality for oligonucleotides in MALDI MS. Organic bases have also been used as co-matrixes; however, the most popular matrix, 3-hydroxypicolinic acid, is not compatible with these additives. We have found that the tetraamine spermine as a matrix additive can successfully eliminate cation adduction and lower the detection limits for DNA in the MALDI experiment, without having to resort to desalting steps. The results suggest that multiply protonated spermine molecules function better than ammonium ions in neutralizing oligonucleotides and displacing alkali cations. Protonated spermine is chemically similar to ammonium ions since it binds to the phosphate backbone and releases protons to the phosphate groups. Spermine can be used successfully with the matrixes 6-aza-2-thiothymine and 80% anthranilic acid/20% nicotinic acid but not with 3-hydroxypicolinic acid. The additive also works well for the analysis of metalated DNA.
State problems in programming human-controlled devices (1994)

Many consumer goods are complicated enough to benefit from programmed control. Today's home electronics devices support a wide range of options and controls. At the same time, personal digital assistants and programmable remote controls are now capable of learning and generating control sequences to control a wide range of devices. Unfortunately, most device interfaces are designed for interactive human control rather than programmed control. The paper analyzes state-based obstacles to programming devices designed for interactive human control. It develops a theory of statelock, a condition in which a control program is unable to synchronize with the state machine underlying the controlled device. The paper also presents design strategies to avoid statelock and applies these strategies to the home audio/video and telephone autodialer domains.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Construction of a vaccinia virus deficient in the essential DNA repair enzyme uracil DNA glycosylase by a complementing cell line (1997)

The vaccinia virus D4R open reading frame, encoding the essential DNA repair enzyme uracil DNA glycosylase, was expressed in two permanent cell lines, the rabbit kidney cell line RK13 and the human fibroblast cell line 293. The temperature-sensitive vaccinia virus mutant ts4149, which maps within D4R, was able to grow under restrictive conditions in both of these transformed cell lines. Cell clones complemented D4R function to various degrees, demonstrating complementation of an essential vaccinia virus gene by a cell line constitutively expressing the essential function. Thus, the complementing host cells allowed the rescue of a virus defective in the D4R gene, demonstrating that this system may be used for the propagation of defective cytoplasmic DNA viruses. The defective virus grew to high yields only in the engineered cell lines. The data support the hypothesis that early gene products, such as uracil DNA glycosylase, supplied in trans can fully complement essential viral functions.
Temporal Association Between Serum Gelsolin Levels and Clinical Events in a Patient with Severe Falciparum Malaria (1997)

Actin is present in high concentrations in most eukaryotic cells and can polymerize into filaments under physiological buffer conditions. As a result of tissue injury and cell lysis, large quantities of actin are released locally and may obstruct the downstream microvasculature, causing further damage to already injured organs. It has been postulated that this mechanism contributes to the development of the adult respiratory distress syndrome and to the diverse complications of falciparum malaria. Actin scavenging proteins--e.g., gelsolin--counteract the effects of extracellular actin, but the capacity of these plasma proteins can be overwhelmed by massive tissue injury. We examined the temporal relationship between serum levels of gelsolin (and tumor necrosis factor-alpha) and the clinical findings for a patient with severe falciparum malaria. The level of gelsolin decreased and then increased as the patient's status first worsened and then improved. We could not determine whether gelsolin served a biologically important function in this patient's recovery or was simply an epiphenomenon of disease activity. Gelsolin levels may be an early prognostic indicator in patients with a systemic inflammatory response syndrome. Moreover, the potential therapeutic role of recombinant human plasma gelsolin in patients with delayed organ dysfunction that commonly follows a self-limited initial insult merits investigation.
Engineering CRISPR-Cas9 RNA–Protein Complexes for Improved Function and Delivery (2018)

The use of CRISPR-derived, RNA-guided nucleases for genome editing has shown great promise for addressing genetic disease. Encouraging work in cell culture and animal models has demonstrated the capability of genome-editing enzymes to correct disease-causing loci, but clinical translation can only proceed once the corrective enzymes have been rendered safe, effective, and capable of being delivered to the appropriate cells. To address these needs, there has been rapid and extensive progress in the engineering of the RNA and protein components of Cas9, the most widely-used genome editor. Here we review advances in engineering of the enzyme Cas9 by altering its chemical or molecular composition. Such efforts have enhanced enzyme stability, improved capacity for delivery, augmented specificity, and broadened the horizons of genome manipulation.
Rigid-rod polymers. 1. Synthesis and thermal properties of para-aromatic polymers with 2,6-benzobisoxazole units in the main chain (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRigid-rod polymers. 1. Synthesis and thermal properties of para-aromatic polymers with 2,6-benzobisoxazole units in the main chainJames F. Wolfe and F. E. ArnoldCite this: Macromolecules 1981, 14, 4, 909–915Publication Date (Print):July 1, 1981Publication History Published online1 May 2002Published inissue 1 July 1981https://pubs.acs.org/doi/10.1021/ma50005a004https://doi.org/10.1021/ma50005a004research-articleACS PublicationsRequest reuse permissionsArticle Views2174Altmetric-Citations329LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Early onset epileptic encephalopathy caused by de novo SCN8A mutations (2014)

Our data reveal that SCN8A mutations can cause variable phenotypes, most of which can be diagnosed as unclassified EOEEs, and rarely as MMPSI. Together with previous reports, our study further indicates that genetic testing of SCN8A should be considered in children with unclassified severe epilepsy.
Aromatic Ring-Fused BODIPY-Based Conjugated Polymers Exhibiting Narrow Near-Infrared Emission Bands (2009)

Three conjugated copolymers, consisted of alternating p-phenylene-ethynylene and boron di(iso)indomethene units in the backbone, were synthesized via palladium-catalyzed Sonogashira coupling reaction of 1,4-diethynyl-2,5-dihexadecyloxybenzene and three diiodophenyl-fused BODIPY monomers. The structures and properties of the conjugated polymers were characterized by 1H NMR, 11B NMR, and Fourier transform infrared (FT-IR) spectroscopies, elemental analysis, size exclusion chromatography (SEC), UV−vis absorption spectroscopy, photoluminescence (PL) spectroscopy, and theoretical calculation using density-functional theory (DFT) method. The polymers obtained were fusible and soluble in common organic solvents including THF, benzene, toluene, CHCl3, and CH2Cl2, etc. The incorporation of the indomethene monomers into p-phenylene-ethynylene main chain led to red shifts in UV-absorption and PL spectra by extended π-conjugation of the copolymers in comparison with the monomers. Accordingly, the copolymers emitted in the range from deep-red to near-infrared region with emission spectral maxima at around 691−720 nm and exhibited high quantum yields (ΦF = 33−49%). The photostabilities of the polymers were examined by monitoring decrease of the PL spectra under continuous UV irradiation using a UV lamp under aerobic conditions. Further, their thermal stabilities were also investigated by TGA measurement.
Studies on Charge Transport in Self-Assembled Gold−Dithiol Films:  Conductivity, Photoconductivity, and Photoelectrochemical Measurements (2000)

Electronic transport in gold−dithiol nanoparticle films was studied using conductivity, photoconductivity, and photoelectrochemical means. The films were characterized by SEM and optical spectroscopy. GC/MS was used for the analysis of the pyrolysis products during heat treatment. Films were assembled on glass substrates using gold sol and different alkanethiol spacers (1,2-ethanedithiol (C2), 1,5-pentanedithiol (C5), and 1,8-octanedithiol (C8)). Resistance−temperature measurements revealed that the effective activation energies for conduction were 0, 5, and 15 meV for films assembled using C2, C5, and C8 spacers, respectively. Light action spectra of photoconductivity of gold−dithiol nanoparticle films revealed 0.8−1.0 eV threshold photon energy. The difference between the observed threshold energies points to different mechanisms for conductivity and photoconductivity. The low effective activation energy for dark conduction is attributed to a mixed mechanism of conduction, tunneling between insulated particles, and metal conduction through defects which are ascribed to direct contact points between metal particles. The photoconductivity mechanism involves photoemission from metal particles into the insulator layer. Photoelectrochemical studies of gold nanoparticle electrodes in aqueous electrolyte revealed 3.5 eV photon energy threshold of the photocurrent at an electrode potential of E = 0 V vs Ag/AgCl reference. The much higher photoelectrochemical threshold energies are ascribed to direct photoemission processes from the surface metal particles into the electrolyte. Heat treatment of the films decreased film resistance and increased the temperature coefficient of resistance to values approaching that of metal gold. These trends are attributed to pyrolysis of spacer molecules, which favor the metal conduction mechanism.
Chemical Genomics Identifies the Unfolded Protein Response as a Target for Selective Cancer Cell Killing during Glucose Deprivation (2009)

Glucose deprivation, a cell condition that occurs in solid tumors, activates the unfolded protein response (UPR). A key feature of the UPR is the transcription program activation, which allows the cell to survive under stress conditions. Here, we show that the UPR transcription program is disrupted by the antidiabetic biguanides metformin, buformin, and phenformin depending on cellular glucose availability. These drugs inhibit production of the UPR transcription activators XBP1 and ATF4 and induce massive cell death during glucose deprivation as did the antitumor macrocyclic compound versipelostatin. Gene expression profiling shows remarkable similarity in the modes of action of biguanides and versipelostatin determined by the broad range of glucose deprivation-inducible genes. Importantly, during glucose deprivation, most of the biguanide suppression genes overlap with the genes induced by tunicamycin, a chemical UPR inducer. Gene expression profiling also identifies drug-driven signatures as a tool for discovering pharmacologic UPR modulators. Our findings show that disrupting the UPR during glucose deprivation could be an attractive approach for selective cancer cell killing and could provide a chemical genomic basis for developing UPR-targeting drugs against solid tumors.
Transmit selection diversity for multiuser spatial multiplexing systems (2005)

Multiuser spatial multiplexing uses precoding to support multiple users in multi-antenna wireless channels. In this paper, two transmit diversity techniques are proposed that use extra transmit antennas to obtain additional diversity. In one solution, an eigenmode selection technique is proposed to achieve higher diversity gain by optimally matching the data streams to eigenmodes with superior channel conditions. In the other solution, a multiuser antenna selection algorithm is proposed that achieves good performance with fewer RF chains and lower system cost. Selection criteria for single-user spatial multiplexing are extended to the multiuser scenario in the context of unitary downlink precoding, and a novel selection algorithm that is near optimal in terms of symbol error rate (SER) is proposed. Simulation results show dramatic SNR reductions of 6-10 dB at an uncoded SER of 10/sup -3/ with only one extra antenna.