gebhart/patrepeval huggingface dataset:
Corticotropin-releasing hormone: possible role in eating disorders. (1989)

It has been hypothesized that corticotropin-releasing hormone (CRH) is an integral mediator in the pathophysiology of anorexia nervosa. This hypothesis is based on a) studies on patients with eating disorders which found elevated CRH levels in the cerebrospinal fluid and an abnormal response of the hypothalamopituitary (HYPAC) axis to intravenous CRH, and b) the discovery that the central administration of CRH to rats causes an acute anorectic state. Human and animal data supporting this hypothesis are reviewed, and important problems with the data base and the interpretation of these data are discussed. Appropriate directions for future research are highlighted.
Efficient new-view synthesis using pairwise dictionary priors (2007)

New-view synthesis (NVS) using texture priors (as opposed to surface-smoothness priors) can yield high quality results, but the standard formulation is in terms of large-clique Markov random fields (MRFs). Only local optimization methods such as iterated conditional modes, which are prone to fall into local minima close to the initial estimate, are practical for solving these problems. In this paper we replace the large-clique energies with pairwise potentials, by restricting the patch dictionary for each clique to image regions suitable for that clique. This enables for the first time the use of a global optimization method, such as tree-reweighted message passing, to solve the NVS problem with image-based priors. We employ a robust, truncated quadratic kernel to reject outliers caused by occlusions, specularities and moving objects, within our global optimization. Because the MRF optimization is thus fast, computing the unary potentials becomes the new performance bottleneck. An additional contribution of this paper is a novel, fast method for enumerating color modes of the per-pixel unary potentials, despite the non-convex nature of our robust kernel. We compare the results of our technique with other rendering methods, and discuss the relative merits and flaws of regularizing color, and of local versus global dictionaries.
Characterization of two conformational forms of the major DNA-binding protein encoded by herpes simplex virus 1 (1982)

We have resolved two electrophoretic species of the major DNA-binding protein, infected cell polypeptide 8 (ICP8), encoded by herpes simplex virus 1. In pulse-chase experiments, we observed the conversion of the ICP8a form, the slower migrating species, to the faster migrating form, ICP8b. Thus, the two species appear to be related as precursor-product. The conversion was not due to proteolytic cleavage, because higher concentrations of reducing agents in the sample buffer shifted the faster moving form to the slower moving species. Also, the two forms have identical peptide patterns as analyzed by partial proteolysis in sodium dodecyl sulfate. Thus, the faster moving species appears to be a conformational isomer containing intramolecular disulfide bonds. The functional significance of the two forms of the protein is discussed.
Development of a Cocktail of Recombinant‐Expressed Human Rabies Virus–Neutralizing Monoclonal Antibodies for Postexposure Prophylaxis of Rabies (2003)

To provide a cost-effective and safe replacement for human rabies immunoglobulin (HRIG), we used DNA recombinant technology to express 3 human rabies virus-neutralizing human monoclonal antibodies (huMAbs) in a rhabdovirus vector (RhV). Infection of either baby hamster kidney cells or CHO cells, with the resulting RhV-huMAb recombinant viruses, yielded high-level production (< or =40 micro g/mL/48 h) of RhV recombinant-expressed huMAbs (rhuMAbs) that differ in both isotype and epitope-recognition specificity. A cocktail of these rhuMAbs neutralizes several fixed and street wild-type rabies viruses (RVs). Mice and hamsters treated only once with this rhuMAb cocktail after infection with a lethal dose of RV were protected. In the mouse models, the postexposure prophylaxis (PEP) efficacy obtained with the rhuMAb cocktail was comparable to that obtained with HRIG, a finding strongly suggesting that rhuMAbs should be given serious consideration for use in future PEP of humans.
Identification of Staphylococcus aureus Proteins Recognized by the Antibody-Mediated Immune Response to a Biofilm Infection (2006)

Staphylococcus aureus causes persistent, recurrent infections (e.g., osteomyelitis) by forming biofilms. To survey the antibody-mediated immune response and identify those proteins that are immunogenic in an S. aureus biofilm infection, the tibias of rabbits were infected with methicillin-resistant S. aureus to produce chronic osteomyelitis. Sera were collected prior to infection and at 14, 28, and 42 days postinfection. The sera were used to perform Western blot assays on total protein from biofilm grown in vitro and separated by two-dimensional gel electrophoresis. Those proteins recognized by host antibodies in the harvested sera were identified via matrix-assisted laser desorption ionization-time of flight analysis. Using protein from mechanically disrupted total and fractionated biofilm protein samples, we identified 26 and 22 immunogens, respectively. These included a cell surface-associated beta-lactamase, lipoprotein, lipase, autolysin, and an ABC transporter lipoprotein. Studies were also performed using microarray analyses and confirmed the biofilm-specific up-regulation of most of these genes. Therefore, although the biofilm antigens are recognized by the immune system, the biofilm infection can persist. However, these proteins, when delivered as vaccines, may be important in directing the immune system toward an early and effective antibody-mediated response to prevent chronic S. aureus infections. Previous works have identified S. aureus proteins that are immunogenic during acute infections, such as sepsis. However, this is the first work to identify these immunogens during chronic S. aureus biofilm infections and to simultaneously show the global relationship between the antigens expressed during an in vivo infection and the corresponding in vitro transcriptomic and proteomic gene expression levels.
Effect of Enalapril on Survival in Patients with Reduced Left Ventricular Ejection Fractions and Congestive Heart Failure (1991)

The addition of enalapril to conventional therapy significantly reduced mortality and hospitalizations for heart failure in patients with chronic congestive heart failure and reduced ejection fractions.
Gastro‐oesophageal reflux associated with nocturnal gastric acid breakthrough on proton pump inhibitors (1998)

Nocturnal acid breakthrough is frequently seen on proton pump inhibitors twice daily and is often accompanied by oesophageal reflux. This has important implications for medical therapy in patients with severe gastro-oesophageal reflux and Barrett's oesophagus.
Pattern of morbidity and mortality of newborns admitted into the sick and special care baby unit of Enugu State University Teaching Hospital, Enugu state (2014)

The neonatal mortality rate and the causes of death in this study are similar to those documented by other studies in Nigeria and are largely preventable. Strengthening perinatal care, emergency obstetric services, and enhancement of neonatal resuscitation skills to traditional birth attendants (TBAs) and other community health workers are necessary to reduce the neonatal mortality in our setting and other rural settings across developing countries.
Total synthesis of a C15 ginkgolide, (.+-.)-bilobalide (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTotal synthesis of a C15 ginkgolide, (.+-.)-bilobalideE. J. Corey and Wei Guo SuCite this: J. Am. Chem. Soc. 1987, 109, 24, 7534–7536Publication Date (Print):November 1, 1987Publication History Published online1 May 2002Published inissue 1 November 1987https://doi.org/10.1021/ja00258a050RIGHTS & PERMISSIONSArticle Views2169Altmetric-Citations79LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (420 KB) Get e-AlertsSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Drug discovery in academic institutions (2013)

Although academic science has always provided a fundamental understanding of the biological and clinical basis of disease, the opportunity and imperative for academics to contribute more directly to the discovery of new medicines continues to grow. Embedding medicinal chemists with cancer biologists creates collaborative opportunities for drug discovery and the design and synthesis of chemical biology tool compounds (chemical probes) to better elucidate the role of specific proteins and pathways in biology and disease. Two case studies are presented here: (1) the discovery of inhibitors of mer kinase to treat acute lymphoblastic leukemia in children and (2) the discovery of chemical probes targeting epigenetic regulators. These case studies provide lessons in target selection strategies, the requirement for iterative optimization of lead compounds (useful drugs/probes rarely come directly from a screen), and the value of mutually dependent collaborations between medicinal chemists and cancer biologists.
Frequency of Firmicutes and Bacteroidetes in gut microbiota in obese and normal weight Egyptian children and adults (2011)

Introduction: Obesity and associated metabolic disorders are a worldwide epidemic. Recent evidence suggests that the microbial community in the human intestine may play an important role in the pathogenesis of obesity. The aim of this study was to assess the differences in the composition of the intestinal microbiota between obese and normal weight Egyptian children and adults. Material and methods: The study included 79 subjects among whom 51 were obese (23 children and 28 adults), and 28 were subjects of normal weight (17 children and 11 adults). Faecal samples were collected from all subjects. Total DNA was extracted from collected stool samples and submitted to conventional PCR for detection of Firmicutes and Bacteroidetes. All the studied group was subjected to clinical and anthropometric evaluation. Laboratory assessment of fasting glucose, high-sensitivity C-reactive protein (hsCRP) and lipid profile was performed. Results: The proportions of the phyla Firmicutes and Bacteroidetes were statistically significantly increased in the obese group compared to the normal weight group (p < 0.001, p = 0.003 respectively). The study also found a statistically significant positive trend for higher hsCRP in subjects with positive Firmicutes (p = 0.004). However, no associations were found between positive Bacteroidetes and hsCRP. Conclusions: The results of this study indicate that obesity in Egyptian children and adults is associated with compositional changes in faecal microbiota with increase in the phyla Firmicutes and Bacteroidetes. This could be considered when developing strategies to control obesity and its associated diseases by modifying the gut microbiota.
Automatic generation of phone sets and lexical transcriptions (2002)

Large vocabulary automatic speech recognition systems model words as sequences of a small set of basic sub-word units (the phoneset), which the systems are trained to classify. All words in the system's vocabulary are transcribed in terms of this set in a dictionary. The phoneset and dictionary are specific to a language and are typically designed manually. The system's performance is critically dependent on the quality of the phoneset and the accuracy of the dictionary. The authors attempt to generate the phoneset and dictionary automatically, using only the training data and their transcriptions. We treat this as a joint optimization problem with a maximum a posteriori solution for the dictionary and a maximum likelihood solution for the phoneset and its acoustic models. Experiments with the DARPA Resource Management corpus show that the automatically generated phoneset and dictionary result in recognition accuracies close to those obtained using manually designed ones.
An adaptive antenna array for future land mobile satellite terminals (1989)

A unified approach to adaptive antenna array design and transceiver signal processing architectures is proposed for the user segment of the Land Mobile Satellite communications service. It is suggested that a diversity based receiver architecture would allow the coherent recombination of the multipath signal energy present at the mobile terminal site, and thereby enhance system performance for a given link budget. Further, it is recommended that this processing can be most simply achieved if a pilot-based modulation format is utilised, and near instantaneous adaptation to the multipath energy if feedforward signal regeneration techniques are employed in the receiver.
Tunable erbium—ytterbium fiber sliding-frequency soliton laser (1995)

We characterize the soliton-train emission from an Er–Yb-doped fiber loop laser. We discuss the self-starting dynamics and pulse-repetition-rate control in this sliding-frequency soliton laser. We show that the laser truly self-starts after only one cavity round trip. In the steady state the laser emits a closely spaced train of solitons. We also show that the output pulse width may be controlled by the interplay of continuous frequency shifting, bandwidth-limited amplification, and nonlinear polarization rotation of the circulating solitons. The repetition rate is fixed by means of a weak intracavity feedback. The laser is tunable by shifting of the filter wavelength through the whole spectral band of the active fiber.
Silicon/silicon oxide/LPCVD silicon nitride stacks: the effect of oxide thicknoww on bulk damage and surface passivation (2005)

Silicon / thermally grown silicon dioxide / LPCVD silicon nitride stacks were formed to investigate the influence of the oxide thickness on silicon bulk and surface properties after thermal processing. With no oxide, the LPCVD silicon nitride layer causes serious irreversible bulk damage to silicon wafers after a high temperature treatment. A thin oxide layer (~10nm) helps to substantially reduce the damage. A thick oxide (more than 50nm) can help completely eliminate the bulk damage. An increase of surface states was indicated by an increase of emitter the saturation current density for the stacks with thin oxide layers after high temperature treatments. Even after a re-growth of thick oxide layer and forming gas anneal, the stacks previously without oxide layer shows a much higher emitter saturation current value, which indicates silicon nitride causes a serious Si-SiO2 interface damage.
Studies on antiallergic agents. I. Phenyl-substituted heterocycles with a 5-tetrazolyl or N-(5-tetrazolyl)carbamoyl group. (1982)

A series of phenyl-substituted pyrones, pyridines, and pyrimidines bearing a 5-tetrazolyl or N-(5-tetrazolyl) carbamoyl group as an acidic moiety was synthesized. The compounds were tested for antiallergic activity by passive cutaneous anaphylaxis (PCA) assay in rats after oral administration. Among the compounds synthesized. N-(5-tetrazolyl)-6-phenylpyridine-2-carboxamides (53, 54 and 55) were found to display remarkably high potency.
Reduction of atherosclerosis by administration of dehydroepiandrosterone. A study in the hypercholesterolemic New Zealand white rabbit with aortic intimal injury. (1988)

Dehydroepiandrosterone (DHEA) is an endogenous steroid that blocks carcinogenesis, retards aging, and exerts antiproliferative properties. In vitro, it is a potent inhibitor of glucose-6-phosphate dehydrogenase, the first committed step of the pentose phosphate pathway. In man, serum levels of DHEA and its sulfate peak in early adulthood and drop markedly with age. Epidemiologic evidence indicates that low levels of DHEA or its sulfate conjugate are linked to an increased risk of developing cancer or of death from cardiovascular disease. Like cancer, atherosclerosis is a proliferative process characterized by both initiation and promotion phases. This similarity provided a framework in which to study the antiatherogenic effects of DHEA. Rabbits were randomly assigned to four groups. Two groups of rabbits received aortic endothelial injury by balloon catheter and were fed a 2% cholesterol diet for 12 wk. DHEA, 0.5%, was incorporated into the diet of one group receiving the 2% cholesterol diet and endothelial injury and also into the diet of one of the control groups. Animals were killed after 12 wk and aortas, hearts, and livers were studied. Plasma samples were analyzed for total cholesterol, VLDL, LDL, HDL, triglycerides, DHEA, and DHEA-sulfate levels. The atherogenic insult resulted in severe atherosclerosis in animals not treated with DHEA. In those receiving DHEA there was an almost 50% reduction in plaque size (P = 0.006), inversely related to the serum level of DHEA attained. Fatty infiltration of the heart and liver were also markedly reduced. These beneficial actions were not attributable to differences in body weight gain, food intake, total plasma cholesterol or distribution of cholesterol among the VLDL, LDL, or HDL fractions. The results show that high levels of plasma DHEA inhibit the development of atherosclerosis and they provide an important experimental link to the epidemiologic studies correlating low DHEA-sulfate plasma levels with an enhanced risk of cardiovascular mortality.
Short Communication: Renal Tubular Vacuolation in Animals Treated with Polyethylene-Glycol-Conjugated Proteins (1998)

During toxlcologic evaluation of a dimeric PEG-linked protein, tumor necrosis factor binding protein (TNF-bp), vacuolation of renal cortical tubular epithelium was seen in male and female Sprague-Dawley rats (200–300 g) given iv doses of 40, 20, or 10 mg/kg every other day for 3 months. Tubular lesions in rats treated with 20 or 40 mg/kg for 3 months were only partially reversible after a 2-month recovery period. Despite the presence of marked vacuolation, there were no changes in BUN, creatinine, urinalysis parameters, urinary NAG, urinary B2-microglobulin, or fractional sodium excretion. Single iv doses ≥20 mg/kg TNF-bp caused similar but milder changes. However, equivalent doses of PEG alone or the non-PEG-linked TNF-bp did not cause light microscopic evidence of vacuolation. Treatment of rats with another PEG-linked protein of similar molecular weight resulted in similar changes. Immunostaining for TNF-bp revealed positivity in the apical cytoplasm of renal tubular epithelium within 1 h of iv dosing. Immunostaining of kidneys from chronically dosed rats indicated that protein was present in some vacuoles as long as dosing continued; however, kidneys from animals on a reversibility study had vacuoles but no immunostaining for TNF-bp. These results, along with a study that showed more severe lesions with PEG-linked proteins of lower molecular weight and minimal if any lesions with PEG-linked proteins >70 kDa, suggest that TNF-bp is filtered through the glomemlus and that the protein with attached PEG is reabsorbed by the proximal tubules. Vacuolation may be a result of fluid distension of lysosomes due to the hygroscopic nature of PEG. These studies demonstrated that PEG-linked proteins have the capacity to induce renal tubular vacuolation at high doses. However, the change was not associated with alteration of clinical pathology or functional markers.
Growth factor upregulation of a phosphoinositide-coupled metabotropic glutamate receptor in cortical astrocytes (1995)

CNS function depends on a capacity for plasticity during development, following injury, and in response to changing environmental conditions. Functional alterations in signal transduction pathways and in neurotransmitter receptor expression are possible mechanisms for the expression of such plasticity. In the present report, we demonstrate that exposure of astrocytes to specific growth factors alters both the functional activity and the protein levels of a specific glutamate receptor. Exposure of astrocytes to basic fibroblast growth factor, epidermal growth factor, or transforming growth factor-alpha produced marked increases in the ability of metabotropic glutamate receptor (mGluR) agonists to stimulate phosphoinositide hydrolysis. Using Western immunoblotting, we demonstrate that an increase in the levels of one of the phosphoinositide-coupled mGluR subtypes, mGluR5, accompanies the increased ability of mGluR agonists to stimulate phosphoinositide hydrolysis. In contrast, another phosphoinositide-coupled subtype of this receptor family, mGluR1 alpha, was not present at detectable levels in these cultures. The enhanced stimulation of phosphoinositide hydrolysis showed little sensitivity to pertussis toxin, and appeared to be selective to mGluR agonists, as there was not a similar increase in the ability of norepinephrine or carbachol to stimulate phosphoinositide hydrolysis. These findings demonstrate that expression of mGluRs in astrocytes is plastic, and indicate a novel pathway through which specific growth factors may selectively modulate neurotransmitter action.
Fluorescent decay thermometer with biological applications (1980)

The time constant for fluorescent decay of the red R lines of Ruby has previously been shown to be a monotonic function of temperature over a range of more than 200 °C. An optical thermometer using this effect has been constructed and has a resolution of ±0.3 °C. Improvements in digital signal processing may increase this resolution another factor of 16.
Molecular markers in malignant cutaneous melanoma: Gift horse or one‐trick pony? (2005)

The management of malignant cutaneous melanoma is problematic. Current clinical prognostic factors do not adequately predict disease recurrence and overall survival in a significant subset of patients. Adjuvant therapies for melanoma are notoriously toxic and associated with significant morbidity. Furthermore, it has been difficult to predict which patients will respond best to these treatments, if at all. DNA and RNA biomarkers have been developed to help overcome these problems. Biomarkers have been shown to upstage patients with melanoma, but are the assays sensitive and specific enough for clinical use as predictors of disease outcome or treatment response? We review our experience with DNA and RNA biomarkers in terms of their prognostic and predictive capabilities in malignant melanoma and outline their likely role in the future of melanoma staging, surveillance, and treatment.