gebhart/patrepeval huggingface dataset:
Molecular cloning and expression of catrocollastatin, a snake-venom protein from <i>Crotalus atrox</i> (western diamondback rattlesnake) which inhibits platelet adhesion to collagen (1995)

A 50 kDa protein that inhibits platelet adhesion to collagen has been isolated from snake venom of Crotalus atrox (western diamondback rattlesnake) and has been named 'catrocollastatin'. The cDNA cloning of catrocollastatin has been accomplished. A full-length cDNA of 2310 bp with an open reading frame between nucleotides 51 and 1880 was obtained. The deduced amino acid sequence consists of 609 amino acids. The cDNA-predicted amino acid sequence is highly similar to that of haemorrhagic metalloproteinase jararhagin from Bothrops jararaca venom, HR1B from Trimeresurus flavoviridis, Ht-e from C. atrox and trigramin from T. gramineus. Like jararhagin and HR1B, catrocollastatin is a multidomain molecule composed of an N-terminal domain, a metalloproteinase domain, a disintegrin-like domain and a cysteine-rich C-terminal domain. In the disintegrin-like domain, the frequently seen RGD (Arg-Gly-Asp) sequence is replaced by SECD (Ser-Glu-Cys-Asp). This cDNA was expressed in Spodoptera frugiperda (fall armyworm) (Sf9) insect cells using a baculovirus expression system. Like native catrocollastatin, the expressed protein is capable of selectively blocking collagen-induced platelet aggregation. This is the first full-length clone of a high-molecular-mass haemorrhagin to be expressed.
Universal screening test based on analysis of circulating organ-enriched microRNAs: a novel approach to diagnostic screening (2014)

Early disease detection leads to more effective and cost-efficient treatment. It is especially important for cancer and neurodegenerative diseases, because progression of these pathologies leads to significant and frequently irreversible changes in underlying pathophysiological processes. At the same time, the development of specific screening tests for detection of each of the hundreds of human pathologies in asymptomatic stage may be impractical. Here, we discuss a recently proposed concept: the development of minimally invasive Universal Screening Test (UST) based on analysis of organ-enriched microRNAs in plasma and other bodily fluids. The UST is designed to detect the presence of a pathology in particular organ systems, organs, tissues or cell types without diagnosing a specific disease. Once the pathology is detected, more specific, and if necessary invasive and expensive, tests can be administered to precisely define the nature of the disease. Here, we discuss recent studies and analyze the data supporting the UST approach.
Multiplexed detection of microRNAs by tuning DNA-scaffolded silver nanoclusters (2013)

A universal silver-nanocluster method coupled with target-triggered isothermal exponential amplification reaction (TIEAR) is developed for light-up fluorescent detection of multiple microRNAs (miRNAs) in a label-free format. Taking advantage of the interesting feature of the fine-tuned emission spectrum of fluorescent DNA-scaffolded silver nanoclusters (DNA/AgNCs), our proposed assay is designed such that the different miRNA targets are transferred to the different oligonucleotide reporters via the TIEAR, in which unimolecular DNAs designed for different targets are employed as the amplification templates, polymerases and nicking enzymes as mechanical activators and miRNA targets as the trigger. The produced oligonucleotide reporters act as templates for the synthesis of multicolor DNA/AgNC probes, which correspond to different target inputs. This proposed method has been well validated on the multiplex detection of miRNAs and DNAs, as well as in practical application.
Anti-CD2 antibodies induce T cell unresponsiveness in vivo. (1991)

The CD2 receptor functions as an adhesion and signal molecule in T cell recognition. Multimeric binding of CD2 on T cells to its physiologic ligand LFA-3 on cognate partner cells in vitro efficiently augments the antigen-specific T cell signal delivered by the T cell receptor/CD3 complex. The precise contribution of the antigen-nonspecific CD2-LFA-3 interactions to T cell immune responses in vivo, however, has been difficult to assess. Here we analyzed the role of CD2 in the murine immune response using a nondepleting anti-CD2 monoclonal antibody that induces a marked, reversible modulation of CD2 expression on murine T and B cells in situ. This modulation is dose and time dependent, specific for CD2, and does not require the Fc portion of the antibody. Anti-CD2 antibodies [rat IgG1 or F(ab')2] significantly inhibit the CD4+ T cell-mediated response to hen egg lysozyme and the cytotoxic CD8+ T cell response to a syngeneic tumor cell line. In both cases, anti-CD2 antibodies are only effective when administered before or within 24 h after antigen priming. The suppression of the antitumor response corresponds to a sixfold reduction of specific cytotoxic T lymphocyte precursor cells and results in the abrogation of protective antitumor immunity. Anti-CD2 antibodies also affect the humoral immune response to oxazolone: the isotype switch from specific IgM to IgG1 antibodies is delayed, whereas the IgM response is unaltered. In addition, a single antibody injection results in sustained polyclonal unresponsiveness of T cells irrespective of antigen priming and CD2 modulation. These results document that CD2-mediated signals induce a state of T cell unresponsiveness in vivo.
&lt;title&gt;Spatial-carrier phase-shifting technique of fringe pattern analysis&lt;/title&gt; (1991)

The spatial-carrier phase shifting (SCPS) technique that is related to both Fourier transform and phase-shifting methods of calculating the phase is analyzed. In this technique, a large amount of tilt is introduced into an interferogram, so that the phase difference between successive pixels equals (pi) /2. Three successive pixels are used to recover the phase using the adequate standard phase shifting formula. The detailed error analysis is presented. Examples of application of this method in conventional and moire interferometry, as well as in optical triangulation, are shown. As the SCPS technique requires a single frame only for phase calculation, it is capable of making high-speed measurements or performing testing in adverse environments.
Entanglements in polymer solutions under elongational flow: a combined study of chain stretching, flow velocimetry and elongational viscosity (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEntanglements in polymer solutions under elongational flow: a combined study of chain stretching, flow velocimetry and elongational viscosityA. Chow, A. Keller, A. J. Mueller, and J. A. OdellCite this: Macromolecules 1988, 21, 1, 250–256Publication Date (Print):January 1, 1988Publication History Published online1 May 2002Published inissue 1 January 1988https://pubs.acs.org/doi/10.1021/ma00179a048https://doi.org/10.1021/ma00179a048research-articleACS PublicationsRequest reuse permissionsArticle Views424Altmetric-Citations62LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Venous Levels of Shear Support Neutrophil-Platelet Adhesion and Neutrophil Aggregation in Blood via P-Selectin and β<sub>2</sub>-Integrin (1998)

The results are consistent with a model in which venous levels of shear support platelet adherence to neutrophils via P-selectin binding PSGL-1. This interaction alone is sufficient to mediate neutrophil aggregation. Abrogation of platelet adhesion and aggregation requires blocking Mac-1 in addition to PSGL-1 or P-selectin. The described mechanisms are likely of key importance in the pathogenesis and progression of thrombotic disorders that are exacerbated by leukocyte-platelet aggregation.
Molecular biology approaches to evaluate species variation in immunogenicity and antigenicity of zona pellucida proteins. (1996)

Immunocontraception using the glycoproteins of the mammalian zona pellucida (ZP) has held great promise because antibodies specific to ZP antigens would inhibit fertility and not be abortive. It has been shown, however, that some ZP proteins will elicit adverse effects since immunization may affect ovarian follicular development. These effects vary among different mammalian species as well as on the source of the ZP immunogen. Therefore, the use of molecular biology has been essential in identifying specific ZP protein(s) that inhibit fertility without altering ovarian follicular development and in defining the relationships of ZP proteins among different species. Use of recombinant ZP proteins has allowed us to begin to dissect antigenic domains of ZP proteins and to evaluate their potential roles in the fertilization process. Recent studies using recombinant rabbit ZP proteins to immunize cynomolgus monkeys (Macaca fascicularis) have shown that the 55 kDa ZP protein will elicit antibodies that inhibit sperm binding while not altering ovarian function, in contrast to immunization with a recombinant truncated protein of the 75 kDa ZP protein which causes ovarian dysgenesis. The rabbit 55 kDa protein is the homologue of the pig ZP3 alpha sperm receptor and the human ZPB protein but is distinct from the mouse ZP3 sperm receptor. Expression of this protein using the baculovirus expression system has further shown that the 55 kDa protein binds to capacitated rabbit spermatozoa over the acrosomal region and induces the acrosome reaction. Antibodies against this recombinant form of the ZP also inhibit rabbit spermatozoa from binding to rabbit egg in vitro. These studies demonstrate the need to determine the structure and function of ZP proteins of different mammalian species to evaluate their potential for contraceptive vaccines.
Activation of the human beta-interferon gene requires an interferon-inducible factor. (1986)

beta-Interferon (beta-IFN) gene expression can be induced by poly(I)-poly(C) or virus, but there is considerable variation in the extent of induction between different cell lines. We characterized two poorly inducible human cell lines, HeLa and 143 thymidine kinase negative (143 tk-), to define cellular factors involved in the activation of the beta-IFN gene. We show that the deficiency in beta-IFN induction in these cells can be complemented by fusion to highly inducible mouse cells. We conclude that the human cells are deficient in a trans-acting factor required for B-IFN gene activation. The level of induction of the beta-IFN gene in HeLa and 143 tk- cells can also be increased by priming with IFN before induction. If IFN priming is carried out in the presence of cycloheximide, a approximately 200-fold increase in induction is observed. We conclude that activation of the beta-IFN gene requires an IFN-inducible factor that is only expressed at low levels in unprimed HeLa and 143 tk- cells.
Feature-Based Texture Display for Detection of Polyps on Flattened Colon Volume (2005)

This paper presents a volume-based algorithm to flatten the colon. Based on the flattened colon volume, three different display schemes are adapted to transfer the three-different imensional (3D) flattened colon volume into a 2D image. One display scheme is surface-based rendering, one is volume-based rendering, and the third one is feature-based texture display. These three displays generate not only a traditional flattened-colon surface image, but also a feature-based texture image which can be utilized to characterize and detect the colonic polyps, resulting in a new way to visualize the entire colon.
Rheb Interacts with Raf-1 Kinase and May Function To Integrate Growth Factor- and Protein Kinase A-Dependent Signals (1997)

Rheb is a recently described member of the Ras family that was originally identified as an immediate-early gene in brain but is also widely expressed in other tissues. Here we demonstrate that Rheb interacts with and appears to regulate Raf-1 kinase, an essential component of the H-Ras signaling pathway. In direct contrast to H-Ras, however, the interaction of Rheb with Raf-1 is potentiated by growth factors in combination with agents that increase cyclic AMP (cAMP) levels. Protein kinase A-dependent phosphorylation of serine 43 within the regulatory domain of Raf-1 reciprocally potentiates its interaction with Rheb and decreases its interaction with H-Ras. A single amino acid in the G2 effector domain is critical for the differential properties of Rheb. Since Rheb is an immediate-early gene, our studies suggest that Rheb functions in concert with H-Ras to dynamically integrate cAMP and growth factor signaling.
Experimental fixed and mobile multibeam satellite communications system (2003)

The authors describe the experimental systems for fixed and mobile satellite communications in the ETS-VI (Engineering Test Satellite VI) program. The objectives of the experiments are space confirmation of the onboard equipment performance necessary for multibeam system implementation and function and performance evaluation of the total system including satellite and earth stations. The multibeam system uses three frequency bands: Ka-band (30/20 GHz), C-band (6/4 GHz), and S-band (2.6/2.5 GHz). A number of new techniques have been applied to enhance the multibeam and satellite switch advantages, including satellite-switch time division multiple access incorporated with demand assigned satellite switch operation, cross-band interconnection associated with cross-band diversity, single-beam/multibeam system interconnection, and band-selective switching. A new multibeam system, called the dynamic channel assigned multibeam satellite system, proposed for the mobile communications system to enhance traffic flexibility, is described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Tissue-specific expression of a human polymorphic epithelial mucin (MUC1) in transgenic mice. (1992)

The human MUC1 gene codes for the core protein of a mucin which is expressed by glandular epithelia and the carcinomas which develop from these tissues. The core protein is aberrantly glycosylated in cancers, and some antibodies show specificity in their reactions with the cancer-associated mucin, which also contains epitopes recognized by T-cells from breast and pancreatic cancer patients. For evaluating the potential use of mucin-reactive antibodies and mucin-based immunogens in cancer patients, a mouse model, expressing the MUC1 gene product PEM (polymorphic epithelial mucin) as a self antigen, would be extremely useful. To this end, we have developed transgenic mouse strains expressing the human MUC1 gene product in a tissue-specific manner. The TG4 mouse strain was established using a 40-kilobase fragment containing 4.5 kilobases of 5' and 27 kilobases of 3' flanking sequence. The TG18 strain was developed using a 10.6-kilobase SacII fragment from the 40-kilobase fragment; this fragment contained 1.6 kilobases of 5' sequence and 1.9 kilobases of 3' flanking sequence. Both strains showed tissue specificity of expression of the MUC1 gene, which was very similar to the profile of expression seen in human tissues. The antibody SM-3 is directed to a core protein epitope, which is selectively exposed in breast cancers and which shows a more restricted distribution on normal human tissues. It was established that the distribution of the SM-3 epitope of PEM in the tissues of the transgenic mice is similar to that seen in humans. The transgenic mouse strains described here should form the basis for the development of a preclinical model for the evaluation of PEM-based antigens and of antibodies directed to PEM in cancer therapy.
Novel procedures for isolating intact retinal vascular beds from diabetic humans and animal models. (1993)

Elastase, which exhibits broad protease activity, and not trypsin, is the most important enzyme of the standard, crude trypsin digestion procedure for removal of the nonvascular tissues of the retina.
Raf‐1 expression may influence progression to androgen insensitive prostate cancer (2005)

We conclude that the HER2/Raf-1/AP-1 axis may promote the development of AIPC, leading to early relapse. Members of the pathway may act as novel therapeutic targets for patients.
Total Synthesis of (+)-Paulownin (1997)

(+)-Paulownin, a furofuran lignan from Paulownia tomentosa, was stereoselectively synthesized from (R)-(+)-3-hydroxybutanolide in 12 steps with a yield of 4.4%.
High-temperature CVD silicon films for crystalline silicon thin-film solar cells (2003)

Im Ansatz der kristallinen Silizium Dünnschichtsolarzelle spielt die Abscheidung von Silizium-Schichten eine zentrale Rolle. So erfordert eine industrielle Fertigung von Silizium Dünnschichtsolarzellen die Entwicklung geeigneter Systeme zur effizienten und kostengünstigen Abscheidung von Siliziumschichten.&lt;br /&gt;Am Fraunhofer ISE wurde ein Si-APCVD System konzipiert und aufgebaut, welches den Anforderungen an Schichtqualität und Abscheidekosten entgegen kommt. Im Rahmen der vorliegenden Arbeit wurden für diesen Reaktor verschiedene Si-Abscheideprozesse entwickelt und optimiert. In einem ersten Solarzellen-Ansatz wurden Basis-Schichten auf verschiedene elektrisch inaktive Si-Substrate epitaktisch abgeschieden und sowohl mit Reinraum als auch mit Industrie-nahen Fertigungstechnologien prozessiert. Unter Verwendung von Siebdruck-Techniken konnten mit diesem Zellkonzept Wirkungsgrade bis zu 12.2% und 11.7% für Cz-Si bzw. mc-Si Substrate erreicht werden. Zum erstenmal wurden einkristalline Reclaim Wafer als Substratmaterial eingesetzt. Die beste epitaktische Dünnschichtsolarzelle erreichte hierbei einen Wirkungsgrad von 11.5%. In einem zweiten Ansatz wurden Dünnschichtsolarzellen auf isolierenden Fremdsubstraten hergestellt. Vier Hochtemperatur-Keramiken wurden auf ihre Eignung als Substratmaterial untersucht: heiß gepresstes Siliziumnitrid und Silizium-infiltriertes Siliziumcarbid mit polierten Oberflächen ("ideale" Materialien), und foliengezogenes Siliziumnitrid sowie foliengezogenes SiAlON ("realistische" kostengünstige Materialien). Der beste Wirkungsgrad von 10.7% wurde für eine Solarzelle auf Siliziumcarbid Substrat erreicht.
The Evolving State (2008)

Thesis:The trade order of states follows the constitutional order of states. The current form of the State – the nation-state – is in a process of transformation. To understand the contours of the next trade order of states, we need to identify the causes of the changing constitutional order of states and explore how that constitutional order will engender a new trade order of states.
Activity-aware recommendation for collaborative work in operating rooms (2012)

This paper presents a recommender system for teams of medical professionals working collaboratively in hospital operating rooms. The system recommends relevant virtual actions, such as retrieval of information resources and initiation of communication with professionals outside the operating rooms. Recommendations are based on the current state of the ongoing operation as recognised from sensor data using machine learning techniques. The selection and non-selection of virtual actions during operations are interpreted as implicit feedback and used to update the weight matrices that guide recommendations. A pilot user study involving medical professionals indicates that the adaptation mechanism is effective and that the system provides adequate recommendations.
Clinical safety of HIV lipopeptides used as vaccines in healthy volunteers and HIV-infected adults (2006)

These data demonstrate that reactogenicity and systemic safety of HIV lipopeptides vaccine are acceptable both in healthy volunteers and HIV-infected adults.
Synthesis of Polythiophene-<i>graft</i>-PMMA and Its Role as Compatibilizer for Poly(styrene-<i>co</i>-acrylonitrile)/MWCNT Nanocomposites (2007)

A new compatibilizer, polythiophene-graft-poly(methyl methacrylate) (PMMA), for poly(styrene-co-acrylonitrile) (SAN)/multiwalled carbon nanotube (MWCNT) composite is synthesized. Fluorescence emission spectra and Raman spectra indicate that the polythiophene part of the compatibilizer strongly interacts with the surface of MWCNT, while differential scanning calorimeter (DSC) thermograms show that the PMMA graft of the compatibilizer is miscible with the SAN matrix. The use of new compatibilizer yields well-dispersed SAN/MWCNT nanocomposites with enhanced mechanical properties, whereas the simple mixture of SAN/MWCNT prepared without the addition of the compatibilizer shows an aggregation of MWCNT in SAN matrix. Particularly, SAN/MWCNT nanocomposites in the presence of the compatibilizer show dramatically improved mechanical properties even with the addition of a very small amount (0.01 wt %) of MWCNT.
Duramycin enhances chloride secretion in airway epithelium (1990)

The effect of duramycin, a polypeptide antibiotic, on Cl- transport in canine tracheal epithelium mounted in Ussing chambers was studied. Over a narrow concentration range, duramycin increased short-circuit current (Isc) and net Cl- secretion and had no effect on mannitol flux when added to the mucosal bathing solution. The maximum increase in Isc was observed at a duramycin concentration of 2 X 10(-6) M and was associated with an increase in both unidirectional Cl- fluxes. Higher duramycin concentrations produced a decrease in Isc. Submucosal addition of duramycin had no effect on Isc except at high concentrations. Pretreatment of tissues with mucosal amiloride (10(-4) M) to reduce basal Na+ transport had no effect on the subsequent response to duamycin. In other tissues pretreated with 10(-3) M dibutyryl adenosine 3',5'-cyclic monophosphate (cAMP), duramycin produced a further increase in Isc and net Cl- secretion similar to its effect in nonpretreated tissues. In all instances the increase in Isc was entirely accounted for by an increase in net Cl- secretion. We conclude that duramycin increases Isc and Cl- secretion in airway epithelium. Although the mechanism of activation is not known, these data demonstrate that duramycin increases Cl- secretion by a pathway other than cAMP. An understanding of the mechanism of action of duramycin may further our understanding of Cl- secretion regulation in airway epithelium.
Regularized 3D morphable models (2003)

Three-dimensional morphable models of objects classes are a powerful tool in modeling, animation and recognition. We introduce the new concept of regularized 3D morphable models, along with an iterative learning algorithm, by adding in the statistical model a noise/regularization term which is estimated from the examples set. With regularized 3D morphable models we are able to handle missing information, as it often occurs with data obtained by 3D acquisition systems; additionally, the new models are less complex than, but as powerful as the non-regularized ones. We present the results obtained for a set of 3D face models and a comparison with the new ones obtained by a traditional morphable model on the same data set.