gebhart/patrepeval huggingface dataset:
TLR3 and TLR4 expression in healthy and diseased human endometrium (2008)

Our data suggest an involvement of TLR3 and TLR4 in endometrial diseases as demonstrated by altered expression levels in endometriosis and endometrial cancer.
Mesomorphic, dielectric, and optical properties of fluorosubstituted biphenyls, terphenyls, and quaterphenyls (2008)

Abstract Compounds with moderate and large negative dielectric anisotropy (Δɛ) are very attractive liquid crystal (LC) for vertical alignment mode (VA). Materials with such properties can be achieved by lateral substitution of a polar group into a mesogenic molecule. We synthesized some new LC materials with a negative value of Δɛ, a moderately high birefringence (Δn), and a low viscosity. The mesomorphic and physical behaviour of the novel biphenyls, terphenyls and quaterphenyls fluorosubstituted in the rigid core and also with fluorinated alkyl and alkoxy chains are investigated. The prepared series of four LC compounds are promising for new LC mixtures for various applications. Examples of nematic mixtures with Δɛ∼−3.25 will be presented.
Tight junctions (1998)

Tight junctions are the most apical intercellular junctions of epithelial and endothelial cells and create a regulatable semipermeable diffusion barrier between individual cells. On a cellular level, they form an intramembrane diffusion fence that restricts the intermixing of apical and basolateral membrane components. In addition to these well defined functions, more recent evidence suggests that tight junctions are also involved in basic cellular processes like the regulation of cell growth and differentiation.
Synthesis and muscarinic activities of quinuclidin-3-yltriazole and -tetrazole derivatives (1992)

The synthesis of 15 methyl or unsubstituted 1,2,3-triazoles, 1,2,4-triazoles, and tetrazoles additionally substituted with a 1-azabicyclo[2.2.2]octan-3-yl group is described. The potency and efficacy of these compounds as muscarinic ligands were determined in radioligand binding assays using [3H]oxotremorine and [3H]quinuclidinyl benzilate. Potency and efficacy were found in compounds in which the azole moiety was attached to the azabicyclic ring either through a carbon atom or a nitrogen atom. Electrostatic potential maps of both the C-linked and the novel N-linked series of compounds were calculated. A relationship between position and depth of the electrostatic minima relative to the azabicyclic ring and the potency and efficacy of the compounds was determined.
Sequences Necessary for Nitrate-Dependent Transcription of Arabidopsis Nitrate Reductase Genes (1997)

Nitrate increases the transcription of the two Arabidopsis thaliana nitrate reductase genes. We demonstrated previously that 238 and 330 bp of the 5' flanking regions, designated as NP1 and NP2, of the two nitrate reductase genes NR1 and NR2, respectively, are sufficient for nitrate-dependent transcription (Y. Lin, C.-F. Hwang, J.B. Brown, C.-L. Cheng [1994] Plant Physiol 106: 477-484). Here we identify the cis-acting elements of NP1 and NP2 that are necessary for nitrate-dependent transcription by linker-scanning (LS) analysis. In transgenic plants one LS mutant of NP1 and two LS mutants of NP2 exhibited significantly lower nitrate-induced reporter gene chloramphenicol acetyltransferase activity. To distinguish which of these three mutants lost nitrate inducibility, competitive reverse-transcriptase polymerase chain reaction was used to measure the chloramphenicol acetyltransferase mRNA levels before and after nitrate induction. The single LS mutant in NP1 lost its response to nitrate, whereas the two LS mutants in NP2 partially lost their response to nitrate. A 12-bp sequence is conserved between the NP1 site and the two NP2 sites. This sequence motif is also conserved in the 5' flanking regions of other nitrate-inducible plant genes. Gel mobility shift experiments indicate that these three regions bind to similar proteins. The binding is constitutive with respect to nitrate treatment and was observed in both nonphotosynthetic suspension cells and green leaves.
Thermal Expansion of Microstructured DMPC Bilayers Quantified by Temperature‐Controlled Atomic Force Microscopy (2006)

Microcalorimetry of single bilayers: Temperature-controlled AFM (see images; DMPC=1,2-dimyristoyl-sn-glycero-3-phospocholine) of microstructured lipid bilayers allows for the exact determination of the main phase transition temperature of solid supported membranes. The authors show that changes in membrane thickness, thermal expansion and boundary fluctuations can be determined simultaneously as a function of temperature. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2267/2006/z600013_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Triangulation based topology approach for 2D point sets registration (2014)

Registration is designed to overcome global spatial correspondence ambiguities of different spatial databases and is required as a first stage in integration. It aims at ensuring an accurate and qualitative match while avoiding erroneous or coinciding data-features in the final product. The algorithm presented in this research paper describes an automatic constraint-free robust homological feature based registration of two 2D point sets. The points represent features of interest that exist in spatial databases, e.g. maps and topographic datasets. The algorithm entails an iterative aggregative voting process that consists of a set of qualitative statistical quantifications, based on the correspondences of triangulation structure, aimed at evaluating the geometric similarity of the data. The algorithm is structured to overcome data ambiguities, including data outliers and noise. The automatic aggregative voting algorithm replaces the need for a-priori spatial knowledge or the use of manual or semi-automatic registration that is prone to error. A comparison of the proposed automatic registration with alternative commonly used processes is presented, showing better and robust results. As such, the presented algorithm proves as an important stage towards the qualitative integration and enhancement of spatial databases.
Fabrication and Electrochemical Characteristics of LiFePO&lt;sub&gt;4&lt;/sub&gt; Powders for Lithium-Ion Batteries (2010)

Novel powder fabrication technologies provide opportunities to develop high-performance, low-cost cathode materials for rechargeable lithium-ion batteries. Among various energy storage technologies, rechargeable lithium-ion batteries have been considered as effective solution to the increasing need for high-energy density electrochemical power sources. Rechargeable lithium-ion batteries offer energy densities 2–3 times and power densities 5–6 times higher than conventional Ni-Cd and Ni-MH batteries, and as a result, they weigh less and take less space for a given energy delivery. However, the use of lithium-ion batteries in many large applications such as electric vehicles and storage devices for future power grids is hindered by the poor thermal stability, relatively high toxicity, and high cost of lithium cobalt oxide (LiCoO2) powders, which are currently used as the cathode material in commercial lithium-ion batteries. Recently, lithium iron phosphate (LiFePO4) powders have become a favorable cathode material for lithium-ion batteries because of their low cost, high discharge potential (around 3.4 V versus Li/Li+), large specific capacity (170 mAh/g), good thermal stability, and high abundance with the environmentally benign and safe nature. As a result, there is a huge demand for the production of high-performance LiFePO4 powders. However, LiFePO4 also has its own limitation such as low conductivity (∼10−9 S/cm), which results in poor rate capability. This can be addressed by modifying the powder structure using novel fabrication technologies. This paper presents an overview of recent advances in the fabrication of high-performance LiFePO4 powders for lithium-ion batteries. The LiFePO4 powder fabrication methods covered include: solid-state synthesis, mechanochemical activation, carbothermal reduction, microwave heating, hydrothermal synthesis, sol-gel synthesis, spray pyrolysis, co-precipitation, microemulsion drying, and others. The impacts of these fabrication methods on the structure and performance of LiFePO4 powders are discussed. In addition, the improvement of the conductivity of LiFePO4 powders through novel powder technologies is addressed.
A New Polarimeter for Faint Astronomical Objects (1967)

Photoelectric two-cell polarimeter with rotating achromatic half-wave plane noting wavelength restriction
Linear N≥4-point pose determination (2002)

The determination of the position and the orientation of the camera from the known correspondences of the reference points and the image points is known as the problem of pose estimation in computer vision or space resection in photogrammetry. It is well known that using 3 corresponding points has at most 4 solutions. Less appears to be known about the cases of 4 and 5 points. In this paper, we describe linear solutions that always give the unique solution to 4-point and 5-point pose determination for the reference points not lying on the critical configurations. The same linear method can also be extended to any n/spl ges/5 points. The robustness and accuracy of the method are experimented both on simulated and real images.
Effects of feeding level on growth, composition of gain, carcass quality and mature body size in steers at ages up to six years. (1982)

Tissue gain and efficiency and carcass characteristics of seventy-eight Angus steers were compared on three feed intake regimens and serially slaughtered at 6 months to 6 years. The regimens were: (A) continuous ad ad libitum feeding, (B) restricted to gain about 0.45 kg daily and (C) restricted as in B until 6 months before slaughter then fed ad libitum. Steers, regardless of feeding regimen, made their greatest gains up to 12 months of age and became less efficient after that time. Steers fed ad libitum had the highest percentage of fat but the lowest percentage of lean and bone when compared with steers fed for restricted gain. Carcasses of the steers fed ad libitum contained more lean and fat at each slaughter age. Average daily gain, feed efficiency and percentage of lean and bone decreased with age; and feed intake, slaughter grade, dressing percent, taste panel desirability and percent of fat tended to increase with age. All steers reached their maximum growth in height at withers, depth of chest and length of body at about 3 years. Steers that were restricted up to 6 months before slaughter showed compensatory growth during their ad libitum feeding period and during the first 12 months of the study; protein and fat were produced most efficiently by this growth. It would appear that it is possible to modify fat deposition by changing feed intake level.
IC quality and test transparency (1989)

It is shown that extremely high single-stuck fault coverage is necessary for high-quality products. Even 100% single-stuck fault coverage may not guarantee adequate quality. Results are presented that extend previous work and show that for high required IC quality, process yield has a negligible effect on required test thoroughness. The extensions consist of: removing the assumption of a one-to-one correspondence between chip defects and single-stuck faults; demonstrating that for high quality levels the dependence of quality on test coverage is linear rather than exponential and that for high yields, the dependence of quality on yield is also linear; and showing that the yield used in the calculations should be functional rather than die yield. The theoretical results are compared with data obtained from measurements at a production IC facility.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Silicon waveguide two-photon absorption detector at 1.5 μm wavelength for autocorrelation measurements (2002)

We describe the use of a silicon waveguide for two-photon absorption measurements in an autocorrelator which achieved a peak-power average-power sensitivity of 1 (mW)2 at 1.56 μm wavelength and which was used to measure optical pulses generated by a mode-locked fiber ring laser. The experimental results agreed with a theoretical model of the two-photon-induced photocurrent generation inside the waveguide.
Dendritic cells genetically engineered to express IL-4 inhibit murine collagen-induced arthritis (2001)

Dendritic cells (DCs) are specialized antigen-presenting cells that migrate from the periphery to lymphoid tissues, where they activate and regulate T cells. Genetic modification of DCs to express immunoregulatory molecules would provide a new immunotherapeutic strategy for autoimmune and other diseases. We have engineered bone marrow-derived DCs that express IL-4 and tested the ability of these cells to control murine collagen-induced arthritis (CIA), a model for rheumatoid arthritis in which Th1 cells play a critical role. IL-4-transduced DCs inhibited Th1 responses to collagen type II in vitro. A single injection of IL-4-transduced DCs reduced the incidence and severity of CIA and suppressed established Th1 responses and associated humoral responses, despite only transient persistence of injected DCs in the spleen. In contrast, control DCs and IL-4-transduced T cells or fibroblastic cells failed to alter the course of the disease. The functional effects correlated well with the differential efficiency of DC migration from various sites of injection to lymphoid organs, especially the spleen. The ability of splenic T cells to produce IL-4 in response to anti-CD3 was enhanced after the administration of IL-4-transduced DCS: These results support the feasibility of using genetically modified DCs for the treatment of autoimmune disease.
Monoclonal antibody analysis of human T lymphocyte subpopulations exhibiting autologous mixed lymphocyte reaction. (1981)

In autologous mixed lymphocyte reaction (MLR), T cells proliferate in response to the stimulation by autologous non-T cells. In the present study, human T cell subpopulations, defined by murine monoclonal antibodies OKT4, OKT8, or 9.3, were examined for their capacity to proliferate in autologous and allogeneic MLR. It was observed that the treatment of responder T cells with OKT4 or 9.3 antibody (both defining helper/inducer T cells) and complement (C') ablated their proliferative response in autologous MLR and markedly reduced their allogeneic MLR proliferative response. In contrast, treatment of T cells with OKT8 antibody (defining suppressor/cytotoxic T cells) and C' had no or minimal effect on their proliferative response in autologous MLR. Furthermore, OKT4, 9.3, 9.6 (PAN), or 7.2 anti-human Ia (framework specific) but OKT8 antibody, when present during the entire culture period, in the absence of C' inhibited in a dose-dependent manner the proliferative response in both autologous and allogeneic MLR. Inhibition of proliferation was also observed when the responder T cells were pretreated with OKT4 or 9.3 antibody, washed, and then stimulated with irradiated autologous or allogeneic non-T cells. Pretreatment of T cells with OKT8 or 7.2 anti-Ia antibody in the absence of C' did not influence their proliferative response in autologous MLR. Thus, T cells containing cells with helper/inducer activity defined by OKT4 or 9.3 antibody appear to be the major responder T-cell subpopulation in autologous MLR.
Isopeptide linkage between nonhistone and histone 2A polypeptides of chromosomal conjugate-protein A24. (1977)

Chromosomal protein A24 has a unique structure inasmuch as it contains histone 2A and a nonhistone polypeptide the sequence of which has been partially determined. Comparative analysis of the ninhydrin-insensitive amino-terminal tryptic peptides of protein A24 and histone 2A and a quantitative analysis of their carboxyl-terminal amino acid indicated that protein A24 has two amino termini and one carboxyl terminus. The amino acid sequence analysis of tryptic peptide 17' of protein A24: (see text) showed it contains tryptic peptide 17 of histone 2A, Lys-Thr-Glu-Ser-His-His-Lys. Lysine 119, the amino terminus of this peptide, which is derived from the histone 2A portion of protein A24, is linked by an isopeptide bond to the carboxyl group of a glycine residue. Accordingly, the branched structure of protein A24 proposed is: (see text).
Participation of tumor necrosis factor in the mediation of gram negative bacterial lipopolysaccharide-induced injury in rabbits. (1988)

Macrophages are induced by LPS to release a number of products that determine the host response during gram negative sepsis. To examine the role of one such substance, tumor necrosis factor (TNF), in mediating LPS-induced injury, we employed a rabbit model of endotoxic shock to (a) determine the kinetics and extent of release of TNF into plasma after injection of LPS, and (b) to evaluate the protective effect of in vivo neutralization of LPS-induced TNF by prior infusion of anti-TNF antibody. TNF was maximally induced 45-100 min after injection of 10 micrograms i.v. parent Salmonella minnesota Re595 LPS or 250 micrograms Re595 LPS-HDL complexes. Maximal induction of TNF by LPS was associated with development of hypotension, focal hepatic necrosis, intravascular fibrin deposition and lethality. Based on (a) the peak levels of TNF observed in serum, 2.5 X 10(3) U/ml, (b) the specific activity of purified rabbit macrophage-derived TNF, 1 X 10(8) U/mg, and (c) the biphasic disappearance of intravenously injected purified TNF (t1/2 = 0.5 min, 11 min) we constructed a kinetic model showing that at least 130 micrograms of TNF (1.3 X 10(7) U) was released into plasma 30-200 min postinjection of LPS. Prior infusion of anti-TNF antibody (30-45 min before LPS injection) resulted in neutralization of the LPS-induced serum TNF activity and provided significant protection from the development of hypotension, fibrin deposition, and lethality. Thus, these results provide further evidence that TNF plays a central role mediating the pathophysiologic changes that occur during gram negative endotoxic shock.
Regulation of bile acid synthesis (1998)

Bile acids are important physiological agents required for disposal of cholesterol and absorption of vitamins and fats. Bile acids are synthesized from cholesterol in the liver. Enterohepatic circulation of bile acids is very efficient and plays an important physiological role in lipid absorption and secretion, and regulation of bile acid biosynthesis and cholesterol homeostasis. Conversion of cholesterol to bile acids requires 15 different enzymatic steps. Four cytochrome P450 enzymes play important roles in bile acid biosynthesis. The classic bile acid biosynthesis pathway starts with modification of the sterol ring and followed by side chain cleavage reactions to synthesize cholic acid (CA) and chenodeoxycholic acid (CDCA), the primary bile acids in most species. The first and rate-limiting enzyme in this pathway is cholesterol 7alpha -hydroxylase, a microsomal cytochrome P450, CYP7A. Another microsomal cytochrome P450 sterol 12alpha-hydroxylase (CYP12) is required for the synthesis of cholic acid. Mitochondrial cytochrome P450 sterol 27-hydroxylase (CYP27) catalyzes sterol side chain oxidation to convert C27 sterol to C24 bile acids. An alternative bile acid biosynthesis pathway (acidic) has been known for sometime but only recently has attracted much attention. In this pathway, side chain oxidation precedes modification of the sterol ring. Mitochondrial sterol 27-hydroxylase (CYP27) catalyzes the first reaction and followed by 7alpha-hydroxylation catalyzed by a microsomal oxysterol 7alpha-hydroxylase (CYP7B). Recent advances in purification and cloning of these major enzymes in the pathways have led to better understanding the molecular basis of regulation of bile acid synthesis and physiological role of the alternative pathways.
Antibodies to a Full-Length VAR2CSA Immunogen Are Broadly Strain-Transcendent but Do Not Cross-Inhibit Different Placental-Type Parasite Isolates (2011)

The high molecular weight, multidomain VAR2CSA protein mediating adhesion of Plasmodium falciparum-infected erythrocytes in the placenta is the leading candidate for a pregnancy malaria vaccine. However, it has been difficult so far to generate strong and consistent adhesion blocking antibody responses against most single-domain VAR2CSA immunogens. Recent advances in expression of the full-length recombinant protein showed it binds with much greater specificity and affinity to chondroitin sulphate A (CSA) than individual VAR2CSA domains. This raises the possibility that a specific CSA binding pocket(s) is formed in the full length antigen and could be an important target for vaccine development. In this study, we compared the immunogenicity of a full-length VAR2CSA recombinant protein containing all six Duffy binding-like (DBL) domains to that of a three-domain construct (DBL4-6) in mice and rabbits. Animals immunized with either immunogen acquired antibodies reacting with several VAR2CSA individual domains by ELISA, but antibody responses against the highly conserved DBL4 domain were weaker in animals immunized with full-length DBL1-6 recombinant protein compared to DBL4-6 recombinant protein. Both immunogens induced cross-reactive antibodies to several heterologous CSA-binding parasite lines expressing different VAR2CSA orthologues. However, antibodies that inhibited adhesion of parasites to CSA were only elicited in rabbits immunized with full-length immunogen and inhibition was restricted to the homologous CSA-binding parasite. These findings demonstrate that partial and full-length VAR2CSA immunogens induce cross-reactive antibodies, but inhibitory antibody responses to full-length immunogen were highly allele-specific and variable between animal species.
Characterization and quantification of red cell lipids in normal man (1964)

Human red cell lipids have been studied in a series of normal individuals. Cholesterol comprises 25% of total red cell lipid, and free fatty acid is present. Evidence is presented that the total amount of lipid phosphorus per average red cell is 1.40 × 10−11 mg and that most extraction procedures fail to extract 8% or more (some as much as 40–50%) of red cell phospholipid.The average percentage distribution of the individual phospholipids was: choline glycerophosphatides 30% sphingomyelin 24%, ethanolamine glycerophosphatides 26%, and serine glycerophosphatides 15%. Other minor phospholipid components were also identified. The normal range for total red cell plasmalogen was found to be 4.65–5.85 × 10−11 μmole/cell. Evidence for the presence of a carbohydrate-containing lipid which was eluted from silicic acid columns with serine and inositol glycerophosphatides is also given.The fatty acid distribution of each major phospholipid fraction is characteristic. Ethanolamine glycerophosphatides are high in 20:4, 22:5, and 22:6, while serine and inositol glycerophosphatides contain large amounts of 18:0 and 20:4. Lecithin is distinguished by 20–25 moles % 18:2 and sphingomyelin by high concentrations of 24:0 and 24:1.