gebhart/patrepeval huggingface dataset:
Myoepithelial and basement membrane antigens in benign and malignant human breast tumors (1993)

Serial cryostat sections of 160 human breast lesions and of 9 lymph-node metastases were studied by indirect immunofluorescence. We used monoclonal antibodies (MAbs) to lining-epithelium-specific keratin 8 and to myoepithelium-specific keratin 17 in combination with polyclonal and monoclonal antibodies to major basement membrane components, laminin, collagen type IV, entactin/nidogen, and large heparan sulfate proteoglycan (perlecan) core protein. Continuous basement membranes adjacent to a basal layer of keratin-17-positive myoepithelial cells were typical for normal, benign and in situ carcinomatous structures. In invasive and metastatic structures, always formed by keratin-8-positive tumor cells, basement membranes were found only rarely and with conspicuous fragmentations. This lack of basement membranes correlated with loss of myoepithelium identified by staining for keratin 17. In comedo structures of invasive ductal carcinomas and in papillary carcinomas, fibrovascular complexes with numerous blood vessels and deposition of basement membrane material were often seen in the stroma. Immunomorphological analysis of 41 cases of doubtful diagnosis at intra-operative biopsy was also performed. A combination of MAbs to keratins 8 and 17, and to basement membrane components, made it possible to distinguish between morphologically similar benign and malignant proliferations and to detect single-cell invasion of the stroma. This combination of antibodies may be recommended as an auxiliary immunomorphological tool for differential diagnosis of intra-operative breast biopsies in dubious cases.
Heat transfer from pin-fin heat sinks under multiple impinging jets (2000)

The enhancement of heat transfer from a discrete heat source using multiple jet impingement of air in a confined arrangement was experimentally investigated. A variety of pin-fin heat sinks were mounted on the heat source and the resulting enhancement studied. Average heat transfer coefficients are presented for a range of jet Reynolds numbers (2000<Re<23000). Two jet-to-jet spacings were investigated and the results were compared to single jets of both the same orifice diameter and orifice area. A total fin effectiveness was computed for the pinned heat sinks relative to the unpinned ones, and was in the range of 3 to 6; the highest value was obtained for the largest nozzle diameter. Results for the average heat transfer coefficient were correlated in terms of Reynolds number, fluid properties and geometric parameters of the heat sinks and the orifice plate.
Allele-Specific Inhibitors of Protein Tyrosine Phosphatases (2005)

Protein tyrosine phosphatases (PTPs) are critical cell-signaling molecules. Inhibitors that are selective for individual PTPs would be valuable tools for dissecting complicated phosphorylation networks. However, the common architecture of PTP active sites impedes the discovery of such compounds. To achieve target selectivity, we have redesigned a PTP/inhibitor interface. Site-directed mutagenesis of a prototypical phosphatase, PTP1B, was used to generate "inhibitor-sensitized" PTPs. The PTP1B mutants were targeted by modifying a broad specificity PTP inhibitor with chemical groups that are sterically incompatible with wild-type PTP active sites. From a small panel of putative inhibitors, compounds that selectively inhibit Ile219Ala PTP1B over the wild-type enzyme were identified. Importantly, the corresponding mutation also conferred novel inhibitor sensitivity to T-cell PTP, suggesting that a readily identifiable point mutation can be used to generate a variety of inhibitor-sensitive PTPs.
Early Prospective Results of the Salto-Talaris™ Total Ankle Prosthesis (2012)

ABSTRACT Background Several fixed-bearing total ankle arthroplasty (TAA) systems are available in the United States (US). We report on the early clinical results of the largest known US cohort of patients who received a Salto-Talaris total ankle replacement for end-stage ankle arthritis. Methods We prospectively followed 67 TAA patients with a minimum clinical follow-up of 2 years. Patients completed standardized assessments, including visual analog scale (VAS) for pain, American Orthopaedic Foot and Ankle Society (AOFAS) Hindfoot score, short form (36) health survey (SF-36), and the short musculoskeletal function assessment (SMFA), along with physical examination, functional assessment, and radiographic evaluation, preoperatively and yearly thereafter through most recent follow-up. Results Implant survival was 96% using metallic component revision, removal, or impending failure as endpoints, with a mean follow-up of 2.81 years. Three patients developed aseptic loosening, all involving the tibial component. Of these, one underwent revision to another fixed-bearing TAA system, one patient is awaiting revision surgery, and the other patient has remained minimally symptomatic and fully functional without additional surgery. Forty-five patients underwent at least one additional procedure at the time of their index surgery. The most common concurrent procedure performed was a deltoid ligament release (n = 21), followed by removal of previous hardware (n = 16) and gastrocnemius recession (n = 11). Eight patients underwent additional surgery following their index TAA, most commonly debridement for medial and/or lateral impingement (n = 4). Patients demonstrated significant improvement in VAS, AOFAS hindfoot, several SF-36 subscales, SMFA, and functional scores at most recent follow-up (p &lt; 0.001). Conclusion Early clinical results indicate that the Salto-Talaris fixed-bearing TAA system can provide significant improvement in pain, quality of life, and standard functional measures in patients suffering from end-stage ankle arthritis. The majority of patients underwent at least one concurrent procedure, most commonly to address varus hindfoot deformity, hardware removal, or equinus contracture. Schweitzer Jr KM, Adams Jr SB, Viens NA, Queen RM, Easley ME, DeOrio JK, Nunley II JA. Early Prospective Results of the Salto-Talaris™ Total Ankle Prosthesis. The Duke Orthop J 2012;2(1):23-34.
Monocyte recruitment and expression of monocyte chemoattractant protein-1 are developmentally regulated in remodeling bone in the mouse. (1997)

Tooth eruption is defined as the movement of a tooth from its site of development within the alveolar bone to its position of function in the oral cavity. It represents an excellent model to examine osseous metabolism as bone resorption and bone formation occur simultaneously and are spatially separated. Bone resorption occurs in the coronal (occlusal) area, whereas bone formation occurs in the basal area. Monocytes are thought to have a significant role in the regulation of osseous metabolism. The goal of this study was to examine the recruitment of monocytes to bone in C57BL/6J mice that are undergoing developmentally regulated bone remodeling. Monocytes were detected by immunohistochemistry and osteoclasts were counted as bone-associated multi-nucleated, tartrate-resistant acid phosphatase (TRAP)-positive cells. Cell numbers were obtained from histological sections of animals sacrificed daily for 14 days after birth; an image analysis system was used for quantification. The results demonstrated that, immediately after birth, there were relatively few monocytic cells. In the area of bone resorption, the number of monocytes increased with time, reaching peaks at 5 and 9 days, and decreased thereafter. A similar pattern was observed for osteoclasts. In the area of bone formation, there was a time-dependent increase in the number of monocytes. In contrast, the number of osteoclasts in this area was highest at the earliest time points and decreased after day 3. To investigate potential mechanisms for the recruitment of monocytes, expression of monocyte chemoattractant protein (MCP)-1 was assessed. The number of MCP-1-positive cells increased with time and was generally proportional to the recruitment of mononuclear phagocytes. Osteoblasts were the principal bone cell type expressing MCP-1. The results demonstrate that the recruitment of mononuclear cells in the occlusal area is associated with bone resorption. In contrast, recruitment of monocytes in the basal area is associated with bone formation and a decrease in the number of osteoclasts. These results suggest that monocytes have different functional roles in areas of bone formation compared with bone resorption. Furthermore, the expression of MCP-1 is developmentally regulated and may provide a mechanistic basis to explain the recruitment of monocytic cells.
An evaluation of tyramide signal amplification and archived fixed and frozen tissue in microarray gene expression analysis (2002)

Archival formalin-fixed, paraffin-embedded and ethanol-fixed tissues represent a potentially invaluable resource for gene expression analysis, as they are the most widely available material for studies of human disease. Little data are available evaluating whether RNA obtained from fixed (archival) tissues could produce reliable and reproducible microarray expression data. Here we compare the use of RNA isolated from human archival tissues fixed in ethanol and formalin to frozen tissue in cDNA microarray experiments. Since an additional factor that can limit the utility of archival tissue is the often small quantities available, we also evaluate the use of the tyramide signal amplification method (TSA), which allows the use of small amounts of RNA. Detailed analysis indicates that TSA provides a consistent and reproducible signal amplification method for cDNA microarray analysis, across both arrays and the genes tested. Analysis of this method also highlights the importance of performing non-linear channel normalization and dye switching. Furthermore, archived, fixed specimens can perform well, but not surprisingly, produce more variable results than frozen tissues. Consistent results are more easily obtainable using ethanol-fixed tissues, whereas formalin-fixed tissue does not typically provide a useful substrate for cDNA synthesis and labeling.
An Easy Synthesis of Aliphatic and Aromatic N-Sulfonyl Aldimines (2000)

N-Arenesulfonyl aldimines were prepared from aliphatic and aromatic aldehydes in a two-step transformation.
The immediate usability of graffiti (1997)

We present four empirical measures of the immediate usability of Graffiti, a character recognizer for pen-based computers. Since speed is fully controlled by the user, we measured the accuracy attainable after minimal exposure. The first measure, 79%, is the inherent accuracy, or the extent to which Graffiti strokes match letters in the Roman alphabet. The other three measures were obtained in a formal experiment. We asked 25 subjects to enter the alphabet five times into a pen-based computer under three conditions: (a) following one minute studying the Graffiti reference chart, (b) following five minutes practicing with Graffiti, and (c) following a one week lapse with no intervening practice. The accuracy was 86%, 97%, and 97%, respectively. These are very respectable figures given the limited exposure of subjects. The third figure represents complete retention following a one-week lapse. We present analyses of the errors on a character-by-character basis, noting that poor performing characters should be emphasized in tutorials and other learning aids for new users. Keywords: Pen-based computing, mobile computing, handwriting recognition, gestural input, stylus input, Graffiti.
A low cost searching device for an ATM adapter (2002)

A key function of an ATM adapter is its ability to sort the incoming traffic from the VPi/VCi field of the cell headers. This has to be done at media speed and requires a hardware assist to keep up with the high speed lines over which ATM cells are transported. Although the device of choice to perform a search is a content addressable memory (CAM) such devices are seldom used, especially in the cost sensitive part of the market, because they require a large die size to be built and are too expensive. However, numerous methods have been devised just using regular R/W RAMs. The most efficient, based on various tree structures, tend to be difficult to manage when updating is considered (especially deletions). Hashing, because of its simplicity, is often preferred. Although "no hash function is perfect" some are better adapted in a particular situation. The paper describes a hash device which makes use of the HEC header field as the hash function, requires a modest RAM space and is able to sort cell traffic at the ingress of an ATM adapter with predictable performance.
Evidence in the Saudi Electronic Transaction System, a Comparative Study with the Uncitral Model Laws (2020)

The revolution in technology and communications has led to multiple developments in Saudi legislation. The Saudi legislator has taken care of the rules of electronic transactions, trade, and electronic stores by setting legal systems that put solutions to their legal problems. The article aims to identify evidence of electronic transactions in the Saudi system. Where the Saudi legislator put it to protect electronic transactions and ensure their authenticity, the research article used the comparative descriptive-analytical method, the results of the study indicated that there are legal rules for electronic signature and electronic record of electronic data for customers in the 2007 Saudi electronic system. The most important recommendations of the article are to establish a special evidence law, for electronic transaction.
A FeIII/Oxo Cubane Contained in an Octanuclear Complex of T Symmetry That Is Stable Over Five Oxidation States (1999)

Encapsulated in a protective [{Fe(pz)3}4] outer layer, which provides the complex with structural stability, the octanuclear complex [Fe8(μ4-O)4(μ-pz)12Cl4] contains a Fe4O4 core (see drawing) that withstands four consecutive reversible one-electron reductions. Iron–oxygen clusters are shown to possess redox properties which rival those of their better studied iron–sulfur analogues. pz=pyrazolate anion.
Space Vector Sequence Investigation and Synchronization Methods for Active Front-End Rectifiers in High-Power Current-Source Drives (2008)

Space vector pulsewidth modulation (PWM) schemes for the active front end of a high-power drive normally produce low-order and suborder harmonics due to the low switching frequency and the drifting of synchronization between the PWM waveform and the rectifier input frequency. To provide a synchronized PWM and achieve the best harmonic performance, different space vector sequences suitable for a current-source converter are investigated in this paper. Details on how to achieve the waveform symmetries with a minimum switching frequency for each sequence are discussed. A thorough comparison of the harmonic performance of different space vector sequences is carried out. An optimum space vector modulation method by switching between two best sequences is proposed to achieve the best line-current total harmonic distortion with reduced switching losses. In addition, two synchronization methods, namely a PWM frame regulation method and a direct digital phase-locked loop synchronization method, are proposed. Both methods are equally effective in providing tight synchronization of the PWM waveform with the rectifier input frequency. The work has been verified in simulation and experiment.
Mesh puppetry (2007)

We present mesh puppetry , a variational framework for detail-preserving mesh manipulation through a set of high-level, intuitive, and interactive design tools. Our approach builds upon traditional rigging by optimizing skeleton position and vertex weights in an integrated manner. New poses and animations are created by specifying a few desired constraints on vertex positions, balance of the character, length and rigidity preservation, joint limits, and/or self-collision avoidance. Our algorithm then adjusts the skeleton and solves for the deformed mesh simultaneously through a novel cascading optimization procedure, allowing realtime manipulation of meshes with 50 K + vertices for fast design of pleasing and realistic poses. We demonstrate the potential of our framework through an interactive deformation platform and various applications such as deformation transfer and motion retargeting.
Mutations of the PATCHED gene in several types of sporadic extracutaneous tumors. (1997)

Patients with basal cell nevus syndrome have a high incidence of multiple basal cell carcinomas, medulloblastomas, and meningiomas. Because somatic PATCHED (PTCH) mutations have been found in sporadic basal cell carcinomas, we have screened for PTCH mutations in several types of sporadic extracutaneous tumors. We found that 2 of 14 sporadic medulloblastomas bear somatic nonsense mutations in one copy of the gene and also deletion of the other copy. In addition, we identified missense mutations in PTCH in two of seven breast carcinomas, one of nine meningiomas, and one colon cancer cell line. No PTCH gene mutations were detected in 10 primary colon carcinomas and eighteen bladder carcinomas.
The role of cytokines in regulating estrogen synthesis: implications for the etiology of breast cancer (2002)

Cytokines, such as IL-6 and tumor necrosis factor (TNF)-alpha, have an important role in regulating estrogen synthesis in peripheral tissues, including normal and malignant breast tissues. The activities of the aromatase, estradiol 17beta-hydroxysteroid dehydrogenase and estrone sulfatase are all increased by IL-6 and TNF-alpha. Prostaglandin E2 may also be an important regulator of aromatase activity in breast tumors. Macrophages and lymphocytes, which invade many breast tumors, are thought to be an important source of factors that can stimulate estrogen synthesis in malignant breast tissues. The co-ordinated stimulation of the activities of the enzymes that are involved in estrogen synthesis offers an explanation for the high concentrations of estrogens that are present in breast tumors.
Inertial-aided KLT feature tracking for a moving camera (2009)

We propose a novel inertial-aided KLT feature tracking method robust to camera ego-motions. The conventional KLT uses images only and its working condition is inherently limited to small appearance change between images. When big optical flows are induced by a camera-ego motion, an inertial sensor attached to the camera can provide a good prediction to preserve the tracking performance. We use a low-grade MEMS-based gyroscope to refine an initial condition of the nonlinear optimization in the KLT. It increases the possibility for warping parameters to be in the convergence region of the KLT. For longer tracking with less drift, we use the affine photometric model and it can effectively deal with camera rolling and outdoor illumination change. Extra computational cost caused by this higher-order motion model is alleviated by restraining the Hessian update and GPU acceleration. Experimental results are provided for both indoor and outdoor scenes and GPU implementation issues are discussed.
Occurrence of plasmids and antibiotic resistance among Campylobacter jejuni and Campylobacter coli isolated from healthy and diarrheic animals (1985)

Serologically defined strains of Campylobacter jejuni and Campylobacter coli from healthy and diarrheic animals were examined for the occurrence of plasmid DNA in association with the antibiotic susceptibility of the bacterial host and the health status of the animal host. Of all campylobacter organisms surveyed, 53% (116 of 200) contained plasmid DNA. A plasmid occurrence rate of 73.8% was obtained for C. coli from healthy pigs, contrasted by lower plasmid occurrence rates for C. coli from diarrheic pigs (30%) and from all diarrheic animals (21.4%). For C. jejuni, in contrast, only 13.6% of healthy cattle contained plasmid DNA, contrasted by a higher plasmid occurrence rate of 31.2% from diarrheic cattle. A high plasmid occurrence rate of 75.8% was observed for C. jejuni from healthy chickens. Campylobacter plasmids ranged in size from less than or equal to 1 to 86 megadaltons. Antibiotic susceptibility for 52 animal isolates (excluding chickens) indicated that most isolates were susceptible to kanamycin, erythromycin, gentamicin, tetracycline, and compound sulfonamide, whereas few were susceptible to bacitracin (19.2%); approximately half were susceptible to ampicillin (55.8%) and streptomycin (51.9%), and no isolates were susceptible to penicillin G. More isolates containing plasmids were resistant to ampicillin, tetracycline, and gentamicin than were isolates not carrying plasmids, there being a statistically significant difference for tetracycline and gentamicin, which suggested that these two antibiotics were probably plasmid mediated. The antibiotic susceptibility patterns of 21 chicken isolates of C. jejuni, by contrast, were different in that most were susceptible to ampicillin in addition to kanamycin, erythromycin, and gentamicin, whereas few wer susceptible to compound sulfonamide, streptomycin, and tetracycline in addition to penicillin G and bacitracin. A 30- or 39-megadalton plasmid, or both, common to many of the chicken isolates was usually associated with tetracycline resistance.
Randomized algorithms for optimizing large join queries (1990)

Query optimization for relational database systems is a combinatorial optimization problem, which makes exhaustive search unacceptable as the query size grows. Randomized algorithms, such as Simulated Annealing (SA) and Iterative Improvement (II), are viable alternatives to exhaustive search. We have adapted these algorithms to the optimization of project-select-join queries. We have tested them on large queries of various types with different databases, concluding that in most cases SA identifies a lower cost access plan than II. To explain this result, we have studied the shape of the cost function over the solution space associated with such queries and we have conjectured that it resembles a 'cup' with relatively small variations at the bottom. This has inspired a new Two Phase Optimization algorithm, which is a combination of Simulated Annealing and Iterative Improvement. Experimental results show that Two Phase Optimization outperforms the original algorithms in terms of both output quality and running time.
Expression of “suppressor of cytokine signalling” (SOCS) genes in the developing and adult mouse nervous system (2000)

Growth factor and cytokine signalling in the developing nervous system has multiple effects, ranging from cell differentiation and cell survival to modulation of cell phenotype. Molecules that can regulate growth factor signalling pathways will therefore be of importance in determining the cellular response to factor stimulation. Members of a recently described gene family, the suppressor of cytokine signalling (SOCS) family, can regulate signalling events downstream of predominantly cytokine stimulation and may have important roles in the nervous system. We have examined the temporal and spatial expression of SOCS-1, SOCS-2, and SOCS-3 in the developing and adult nervous system by use of Northern analysis and in situ hybridisation. All three genes were expressed in the brain, with maximal expression from embryonic day 14 to postnatal day 8 and declining thereafter, with SOCS-2 being the most highly expressed. In situ hybridisation analysis showed that SOCS-1 and SOCS-3 had a low and widespread pattern of expression, whereas SOCS-2 expression was higher and tightly regulated. Its expression pattern indicated that SOCS-2 was expressed exclusively in neurons and that it was switched on developmentally at the time of neuronal differentiation. J. Comp. Neurol. 423:348–358, 2000. © 2000 Wiley-Liss, Inc.