gebhart/patrepeval huggingface dataset:
High-Dose Recombinant Interleukin 2 in the Treatment of Patients With Disseminated Cancer (1986)

Experience with the administration of high doses of interleukin 2 (IL-2) alone is described herein. Ten patients with a variety of malignant disorders unresponsive to conventional treatments were treated with at least 30,000 U/kg of IL-2 by bolus administration three times a day. Patients were treated intravenously or intraperitoneally from four to 21 days in a single course, usually interrupted by a week of recovery. Three of six patients with melanoma experienced an objective regression (greater than 50% decrease in volume); there was no response to treatment in patients with colorectal (0/3) or ovarian (0/1) cancer. Two patients with initial objective regressions who subsequently developed progression were re-treated and one sustained a second partial response. Responses lasted 1, 3, and 7 months without additional treatment. Responses in the three patients with melanoma were in visceral sites (lung, liver, and spleen), as well as cutaneous sites in one patient. Progressive shrinkage of tumors for three to six months after the conclusion of therapy has been noted in two patients. Marked lymphocytic infiltrate was noted in a patient with lesions accessible to repeated biopsies. This study demonstrates that the administration of IL-2 can mediate the regression of established cancer in some patients.
Ribozyme- and Deoxyribozyme-Strategies for Medical Applications (2004)

Ribozymes are catalytically active nucleic acids capable of site-specific cleavage of target mRNAs. They have widely been employed as tools in functional studies and for therapeutic purposes. Different classes of ribozymes distinguished by size and mechanism of action have been discovered in natural systems or obtained by in vitro selection. After an introduction to different types of ribozymes with a special focus on the hammerhead and hairpin ribozyme, major challenges in the process of developing ribozymes for medical purposes will be described in the present review. Subsequently, examples of ribozyme applications in animal models for various diseases including cancer, viral infections, rheumatoid arthritis and cardiovascular diseases will be given. The course of phase I and II clinical trials with ribozymes designed to treat patients with virus infections or cancer will be outlined. Finally, the current significance of ribozymes will be discussed in the light of the emergence of new powerful anti-mRNA strategies, particularly RNA interference (RNAi).
Abstract 150: Color-Coded, Prefilled Syringes Corresponding to the Broselow Tape Decrease Medication Time to Administration and Errors Among Emergency Physicians and Nurses in Simulated Critically Ill Pediatric Patients (2012)

Background: Pediatric resuscitations are inherently stressful and clinicians use various methods of determining drug dosing. The processes of calculation may lead to delay or error in drug administ...
Cloning and characterization of livH, the structural gene encoding a component of the leucine transport system in Escherichia coli (1986)

The physical location of the genetically defined livH gene was mapped in the 17-kilobase plasmid pOX1 by using transposon Tn5 inactivation mapping and further confirmed by subcloning and complementation analysis. These results indicated that the livH gene maps 3' to livK, the gene encoding the leucine-specific binding protein. Moreover, the nucleotide sequence of the livH gene and its flanking regions was determined. The livH gene is encoded starting 47 base pairs downstream from the livK gene, and it is transcribed in the same direction as the livK gene. The livK-livH intergenic region lacks promoter sequences and contains a GC-rich sequence that could lead to the formation of a stable stem loop structure. The coding sequence of the livH gene, which is 924 base pairs, specifies a very hydrophobic protein of 308 amino acid residues. Expression of livH-containing plasmids in minicells suggested that a poorly expressed protein with an Mr of 30,000 could be the livH gene product.
Transforming growth factor beta stimulates mammary adenocarcinoma cell invasion and metastatic potential. (1990)

The experimental metastatic potential of 13762NF mammary adenocarcinoma clone MTLn3 was tested after pretreatment in serum-free medium containing transforming growth factor (TGF) beta 1 at 0-5000 pg/ml. Lung colonies were measured 2 weeks after inoculation in syngeneic F344 rats, and a bell-shaped dose-response curve with 2- to 3-fold increase in number of surface lung metastases was seen. Maximal enhancement occurred at the 50 pg/ml dose level. The effect was specific because addition of neutralizing anti-TGF-beta antibody blocked the stimulatory activity at all levels of TGF-beta 1 pretreatment, but when antibody was given alone, neutralizing anti-TGF-beta antibody had no effect on untreated cells. Increased metastatic potential appears to be from an increased propensity of cells to extravasate as tested in the membrane invasion culture system. MTLn3 cells penetrated reconstituted basement-membrane barriers 2- to 3.5-fold more than did untreated control cells, depending upon length of TGF-beta 1 exposure. Increased invasive potential is apparently due, in part, to a 2- to 6-fold increase in type IV collagenolytic (gelatinolytic) and a 2.4-fold increase in heparanase activity. TGF-beta 1 treatment of MTLn3 cells did not alter their growth rate or morphology in the presence of serum; however, growth was inhibited in serum-free medium. Likewise, adhesion to human umbilical vein endothelial cell monolayers or to immobilized reconstituted basement membrane or fibronectin matrices was unchanged. These results suggest that TGF-beta 1 may modulate metastatic potential of mammary tumor cells by controlling their ability to break down and penetrate basement-membrane barriers.
Clustering and its Application in Requirements Engineering (2008)

Large scale software systems challenge almost every activity in the software development life-cycle, including tasks related to eliciting, analyzing, and specifying requirements. Fortunately many of these complexities can be addressed through clustering the requirements in order to create abstractions that are meaningful to human stakeholders. For example, the requirements elicitation process can be supported through dynamically clustering incoming stakeholders’ requests into themes. Cross-cutting concerns, which have a significant impact on the architectural design, can be identified through the use of fuzzy clustering techniques and metrics designed to detect when a theme cross-cuts the dominant decomposition of the system. Finally, traceability techniques, required in critical software projects by many regulatory bodies, can be automated and enhanced by the use of cluster-based information retrieval methods. Unfortunately, despite a significant body of work describing document clustering techniques, there is almost no prior work which directly addresses the challenges, constraints, and nuances of requirements clustering. As a result, the effectiveness of software engineering tools and processes that depend on requirements clustering is severely limited. This report directly addresses the problem of clustering requirements through surveying standard clustering techniques and discussing their application to the requirements clustering process.
Laser Treatment of Tattoos (1967)

Preliminary experiences with lasers in the treatment of tattoos over a period of more than three years have shown that this is a safe procedure, both for the patient and for the operating personnel, when conducted in specially designed laboratories and with planned safety programs. The laser treatment of tattoos is still, and will continue to be for some time, an investigative treatment and not a conventional treatment.
Optical excitation of nuclear spin coherence in a<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msup><mml:mrow><mml:mtext>Tm</mml:mtext></mml:mrow><mml:mrow><mml:mn>3</mml:mn><mml:mo>+</mml:mo></mml:mrow></mml:msup><mml:mo>:</mml:mo><mml:mtext>YAG</mml:mtext></mml:mrow></mml:math>crystal (2008)

A thulium-doped crystal is experimentally shown to be an excellent candidate for broadband quantum storage in a solid-state medium. The nuclear spin coherence is optically excited, detected, and characterized in such a crystal. The lifetime of the spin coherence---the potential storage entity---is measured by means of a new Raman echo protocol to be about $300\text{ }\ensuremath{\mu}\text{s}$ over a wide range of ground state splittings. This flexibility, which is attractive for broadband operation and well fitted to existing quantum sources, results from the simple hyperfine structure, which contrasts to Pr- and Eu-doped crystals.
Analyses of Mutants of Three Genes that Influence Root Hair Development in Zea mays (Gramineae) Suggest that Root Hairs are Dispensable (1994)

Root hairs are specialized epidermal cells that are thought to play an important role in plant nutrition by facilitating the absorption of water and nutrients. Three maize mutants with abnormal root hair morphologies (rthl, rth2, and rth3) have been isolated from Mutator transposon stocks. All three root hair mutant phenotypes are controlled by single recessive alleles. The rthl mutant initiates normal-looking root hair primordia that fail to elongate. The normal-looking root hair primordia of the rth2 mutant elongate to only approximately one-fifth to one-fourth the length of wild type root hairs. Like rth1 primordia, rth3 primordia undergo little elongation. However, unlike the relatively normal-looking rth1 primordia, rth3 primordia are distinctly abnormal when viewed through a scanning electron microscope. The rth1 mutant exhibits pleiotropic nutrient deficiencies, while the rth2 and rth3 mutants grow vigorously. This finding suggests that under some environmental conditions, root hairs are less important to plant growth than has been previously thought. The rthl, rth2, and rth3 genes have been mapped to chromosomes 1L, 5L, and 1S, respectively, via crosses with BA translocation stocks. The rth2 allele exhibits reduced transmission through the male gametophyte, but a normal rate of transmission through female gametophytes; rth1 and rth3 are transmitted at normal rates.
Microscale Heat Transfer Transduced by Surface Plasmon Resonant Gold Nanoparticles (2007)

Visible radiation at resonant frequencies is transduced to thermal energy by surface plasmons on gold nanoparticles. Temperature in </=10-microliter aqueous suspensions of 20-nanometer gold particles irradiated by a continuous wave Ar+ ion laser at 514 nm increased to a maximum equilibrium value. This value increased in proportion to incident laser power and in proportion to nanoparticle content at low concentration. Heat input to the system by nanoparticle transduction of resonant irradiation equaled heat flux outward by conduction and radiation at thermal equilibrium. The efficiency of transducing incident resonant light to heat by microvolume suspensions of gold nanoparticles was determined by applying an energy balance to obtain a microscale heat-transfer time constant from the transient temperature profile. Measured values of transduction efficiency were increased from 3.4% to 9.9% by modulating the incident continuous wave irradiation.
Molecular self-assembly through hydrogen bonding: supramolecular aggregates based on the cyanuric acid-melamine lattice (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMolecular self-assembly through hydrogen bonding: supramolecular aggregates based on the cyanuric acid-melamine latticeChristopher T. Seto and George M. WhitesidesCite this: J. Am. Chem. Soc. 1993, 115, 3, 905–916Publication Date (Print):February 1, 1993Publication History Published online1 May 2002Published inissue 1 February 1993https://doi.org/10.1021/ja00056a014RIGHTS & PERMISSIONSArticle Views2366Altmetric-Citations196LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (4 MB) Get e-AlertsSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Molecular cloning of Moloney murine sarcoma virus: arrangement of virus-related sequences within the normal mouse genome. (1979)

The unintegrated circular DNA form of Moloney murine sarcoma virus (MSV) has been cloned in bacteriophage lambda. Discrete deletions in the viral genome were shown to occur during propagation of recombinant phage in Escherichia coli. Heteroduplex and restriction enzyme analyses indicated the deletion of tandemly repeated sequences within certain of the cloned MSV DNA inserts. Cloned MSV DNA was used to prepare a probe composed of its acquired cellular (src) sequences, shown previously to be necessary for MSV transformation. Analysis of EcoRI digests of normal mouse cellular DNA revealed the presence of a single 14-kilobase-pair fragment containing these sequences which lacked contiguity with endogenous type C helper viral information of the same cells. Thus, the sarcoma virus-specific sequences of MSV are represented within the normal mouse genome in a manner analogous to that of a cellular gene.
Multilayer pLSA for multimodal image retrieval (2009)

It is current state of knowledge that our neocortex consists of six layers [10]. We take this knowledge from neuroscience as an inspiration to extend the standard single-layer probabilistic Latent Semantic Analysis (pLSA) [13] to multiple layers. As multiple layers should naturally handle multiple modalities and a hierarchy of abstractions, we denote this new approach multilayer multimodal probabilistic Latent Semantic Analysis (mm-pLSA). We derive the training and inference rules for the smallest possible non-degenerated mm-pLSA model: a model with two leaf-pLSAs (here from two different data modalities: image tags and visual image features) and a single top-level pLSA node merging the two leaf-pLSAs. From this derivation it is obvious how to extend the learning and inference rules to more modalities and more layers. We also propose a fast and strictly stepwise forward procedure to initialize bottom-up the mm-pLSA model, which in turn can then be post-optimized by the general mm-pLSA learning algorithm. We evaluate the proposed approach experimentally in a query-by-example retrieval task using 50-dimensional topic vectors as image models. We compare various variants of our mm-pLSA system to systems relying solely on visual features or tag features and analyze possible pitfalls of the mm-pLSA training. It is shown that the best variant of the the proposed mm-pLSA system outperforms the unimodal systems by approximately 19% in our query-by-example task.
Formation of Ordered Nanoscale Semiconductor Dots by Ion Sputtering (1999)

A formation process for semiconductor quantum dots based on a surface instability induced by ion sputtering under normal incidence is presented. Crystalline dots 35 nanometers in diameter and arranged in a regular hexagonal lattice were produced on gallium antimonide surfaces. The formation mechanism relies on a natural self-organization mechanism that occurs during the erosion of surfaces, which is based on the interplay between roughening induced by ion sputtering and smoothing due to surface diffusion.
Template-Based Estimation of Time-Varying Tempo (2006)

We present a novel approach to automatic estimation of tempo over time. This method aims at detecting tempo at the tactus level for percussive and nonpercussive audio. The front-end of our system is based on a proposed reassigned spectral energy flux for the detection of musical events. The dominant periodicities of this flux are estimated by a proposed combination of discrete Fourier transform and frequency-mapped autocorrelation function. The most likely meter, beat, and tatum over time are then estimated jointly using proposed meter/beat subdivision templates and a Viterbi decoding algorithm. The performances of our system have been evaluated on four different test sets among which three were used during the ISMIR 2004 tempo induction contest. The performances obtained are close to the best results of this contest.
Experience with a prototype motion compensated standards converter for down-conversion of 1125/60/2:1 SMPTE-240 M high definition to 625/50/2:1 video (1992)

The authors describe experience gained through the operational use of a motion compensated standards converter for down conversion of 1125/60/2:1 SMPTE-240 M standard high definition to 625/50/2:1 conventional definition video. In particular the use of the equipment during two field trials, at the 25th Montreux Jazz Festival and at the Salzburg Festival, is related. The information derived through these trials has been used to refine aspects of the conversion algorithm and to shape the next generation of converters. The practical realisation of these new converters is also discussed. >
Neisserial Porin-Induced Dendritic Cell Activation Is MyD88 and TLR2 Dependent (2005)

Neisserial porins have been shown to act as B cell mitogens and immune adjuvants. PorA and PorB are the major outer membrane porin proteins of the human pathogen Neisseria meningitidis. We have shown that the mechanism of the immunopotentiating capability of porin involves up-regulation of the T cell costimulatory ligand, CD86. Due to neisserial porin's ability to activate B cells and potentiate immune responses, we hypothesized that porin also employs the potent immune stimulatory function of dendritic cells (DC). We examined the ability of purified N. meningitidis PorB to induce maturation of murine splenic and bone marrow-derived DC. PorB treatment induced DC maturation, as demonstrated by increased expression of CD86 and class I and II MHC molecules. In addition, PorB not only enhanced the allostimulatory activity of DC, but also augmented the ability of DC to stimulate T cells in an Ag-specific manner. PorB-matured DC secreted the inflammatory cytokine IL-6, which may have implications for the adjuvant property of porin. Induction of IL-6 by PorB is also significant because IL-6 is one of a number of cytokines produced during infection with N. meningitidis and may be involved in the inflammatory process observed during infection and disease. We previously demonstrated the requirement of MyD88 and TLR2 for PorB-induced B cell activation. In the present study, MyD88 and TLR2 were also essential for PorB-induced DC activation. This work is significant for elucidating the mechanism(s) of neisserial porin's immune stimulatory activity.
Unique Features of Hepatitis C Virus Capsid Formation Revealed by De Novo Cell-Free Assembly (2004)

ABSTRACT The assembly of hepatitis C virus (HCV) is poorly understood, largely due to the lack of mammalian cell culture systems that are easily manipulated and produce high titers of virus. This problem is highlighted by the inability of the recently established HCV replicon systems to support HCV capsid assembly despite high levels of structural protein synthesis. Here we demonstrate that up to 80% of HCV core protein synthesized de novo in cell-free systems containing rabbit reticulocyte lysate or wheat germ extracts assembles into HCV capsids. This contrasts with standard primate cell culture systems, in which almost no core assembles into capsids. Cell-free HCV capsids, which have a sedimentation value of ≈100S, have a buoyant density (1.28 g/ml) on cesium chloride similar to that of HCV capsids from other systems. Capsids produced in cell-free systems are also indistinguishable from capsids isolated from HCV-infected patient serum when analyzed by transmission electron microscopy. Using these cell-free systems, we show that HCV capsid assembly is independent of signal sequence cleavage, is dependent on the N terminus but not the C terminus of HCV core, proceeds at very low nascent chain concentrations, is independent of intact membrane surfaces, and is partially inhibited by cultured liver cell lysates. By allowing reproducible and quantitative assessment of viral and cellular requirements for capsid formation, these cell-free systems make a mechanistic dissection of HCV capsid assembly possible.
Scottish trust project (1999)

Drawing on his experiences negotiating a major PFI hospital deal in Scotland, the author highlights the potential benefits generated and assesses the future role of such schemes in the NHS.
Asymmetric crystallization and melting kinetics in sodium: A molecular-dynamics study (1990)

Computer-simulation studies of the crystallization and melting of a model of sodium have been carried out to determine the (001) steady-state velocity versus temperature relation in the temperature region 81 to 497 K. Using a form of transition-state theory there is an apparent slope discontinuity in the velocity versus temperature relation at the melting point, which is correlated with slope discontinuities in averaged order parameters for planes buried in the crystal; planes appear to soften more per unit temperature increase above the melting point leading to larger melting velocities.
Comparison of Linear and Nonlinear Kriging Methods for Characterization and Interpolation of Soil Data (2011)

Characterization and analysis of large quantities of existing soil data represent highly complicated tasks because of the spatial correlation, uncertainty, and complexity of the processes underlying soil formation. In this work, three linear kriging (simple kriging, ordinary kriging, and universal kriging) and three nonlinear kriging (indicator kriging, probability kriging, and disjunctive kriging) algorithms are compared to determine which is best suited for the characterization and interpolation of soil data for applications in transportation projects. A spherical model is employed as the experimental variogram to aid the spatial interpolation and cross-validation. The kriged data are subjected to leave-one-out cross-validation. The data used are in both vector and raster format. Statistical measures of correctness (mean prediction error, root-mean-square error, standardized root-mean-square error, average standard error) from the cross-validation are used to compare the kriging algorithms. Using indicator and probability kriging with the vector data set yielded the best results.