gebhart/patrepeval huggingface dataset:
Excluding Pulmonary Embolism at the Bedside without Diagnostic Imaging: Management of Patients with Suspected Pulmonary Embolism Presenting to the Emergency Department by Using a Simple Clinical Model and <scp>d</scp>-dimer (2001)

Managing patients for suspected pulmonary embolism on the basis of pretest probability and D -dimer result is safe and decreases the need for diagnostic imaging.
Method for Optimizing CIELAB (2008)

One of the goals of color appearance models is perceptual uniformity. In a perceptually uniform space, 1 &#x394;E difference between two colors would have similar magnitude visually regardless of the hue, chroma, or lightness of the colors being compared. In reality, there can be significant differences in perceived magnitude, resulting in modified &#x394;E metrics such as &#x394;E94 and &#x394;E2000. This paper proposes a method for improving existing expressions for CIELAB by modifying the matrix converting LMS-&gt;XYZ and the coefficients in CIELAB.
Superficial endometrial vascular fragility in Norplant users and in women with ovulatory dysfunctional uterine bleeding (2000)

The aim of this study was to develop an objective test for superficial endometrial vascular fragility at hysteroscopy, and to apply this test to women using Norplant((R)) for contraception and to women with ovulatory dysfunctional uterine bleeding (DUB). A prospective observational study was carried out in 34 Norplant users and 20 women with menorrhagia due to ovulatory DUB. Superficial endometrial vascular fragility was assessed at hysteroscopy by observing the source and extent of frank and subepithelial bleeding during controlled collapse and redistension of the uterine cavity. Superficial endometrial blood vessels were more fragile in Norplant users compared to women with DUB (chi(2) = 11.60, P = 0.02). Superficial endometrial petechiae (chi(2) = 37.9, P < 0.0001) and ecchymoses (chi(2) = 42.2, P = 0.0001) were more frequently observed in the Norplant users than in the menorrhagia group. In Norplant users, superficial endometrial vascular fragility was increased in those with frequent bleeding or spotting during the past 30 days (chi(2) = 6.15, P = 0.01), and in those who were examined during a bleeding episode (chi(2) = 5.3, P = 0.02). Fragility was increased in the menorrhagia group during the perimenstrual period (days 24 to 05; chi(2) = 12.83, P = 0.01). There was no obvious relationship between subepithelial bleeding and circulating concentrations of oestradiol and progesterone.
Fast Facial Detection by Depth Map Analysis (2013)

In order to obtain correct facial recognition results, one needs to adopt appropriate facial detection techniques. Moreover, the effects of facial detection are usually affected by the environmental conditions such as background, illumination, and complexity of objectives. In this paper, the proposed facial detection scheme, which is based on depth map analysis, aims to improve the effectiveness of facial detection and recognition under different environmental illumination conditions. The proposed procedures consist of scene depth determination, outline analysis, Haar-like classification, and related image processing operations. Since infrared light sources can be used to increase dark visibility, the active infrared visual images captured by a structured light sensory device such as Kinect will be less influenced by environmental lights. It benefits the accuracy of the facial detection. Therefore, the proposed system will detect the objective human and face firstly and obtain the relative position by structured light analysis. Next, the face can be determined by image processing operations. From the experimental results, it demonstrates that the proposed scheme not only improves facial detection under varying light conditions but also benefits facial recognition.
Expression of the chloramphenicol acetyltransferase gene in mammalian cells under the control of adenovirus type 12 promoters: effect of promoter methylation on gene expression. (1983)

The effect of DNA methylation at specific promoter sites on gene expression was tested by using a sensitive and quantitative assay system. The plasmid pSVO CAT contains the prokaryotic gene chloramphenicol acetyltransferase (CAT) and a HindIII site in front of it for experimental promoter insertion. Upon insertion into pSVO CAT, the E1a and protein IX gene promoters from adenovirus type 12 (Ad12) DNA were capable of mediating CAT expression upon transfection in mouse cells. In many viral and nonviral eukaryotic genes, DNA methylation at highly specific sites in the promoter region can attain a regulatory function in gene expression. One of the important sites is the 5' C-C-G-G 3' sequence. The CAT-promoting activity of the early simian virus 40 promoter in plasmid pSV2 CAT is refractory to methylation by the Hpa II or Hha I DNA methyltransferase at 5' C-C-G-G 3' or 5' G-C-G-C 3' sequences, respectively, because this promoter lacks such sites. The CAT coding sequence of this plasmid carries four Hpa II and no Hha I sites. Methylation of the Hpa II sites in the coding region does not affect expression. The E1a promoter of Ad12 DNA comprising the leftmost 525 base pairs of the viral genome carries two 5' C-C-G-G 3' and three 5' G-C-G-C 3' sites upstream from the leftmost "TATA" signal. Methylation of the Hpa II or Hha I sites incapacitates this promoter. The promoter of protein IX gene of Ad12 DNA contains one 5' C-C-G-G 3' and one 5' G-C-G-C 3' site downstream and two 5' G-C-G-C 3' sites greater than 300 base pairs upstream from the TATA motif and probably outside the promoter. The protein IX promoter is not inactivated by methylation of these sites. These data demonstrate that critical 5' methylations in the promoter region decrease or eliminate transcription; methylations of sites too far upstream or probably any sites downstream from the TATA site do not affect expression.
Upgrade of the Electroporation Device KEA-MOBIL (2009)

For the production of synthetic bio-fuel the use of green biomass enables additional resources of raw material supplementary to the commonly used dry material. An electroporation assisted drying process gives the opportunity of a more efficient dewatering than only pressing and subsequent drying. Experiments showed a lower relative humidity of the pressed material after electroporation compared to pressing the raw material only. Moreover, the drying process of the electroporated material was much shorter. This is because the pores formed in the cell membranes foster a better extraction of water during pressing and enhance the diffusion process during the drying process. The paper presents exemplarily the experimental results for green rye. For the electroporation-assisted drying process the electric contact to the plant material has to be established by means of the juice inside the plants only rather than adding additional water. Hence, for experiments in larger scale, the existing electroporation device KEA-MOBIL has been equipped with a new combined electroporation reactor and press. The reactor has a plate electrode system, whose upper electrode additionally serves as a piston to apply mechanical force to extract juice for a good electrical contact between plant material and the electrodes. A two-stage Marx generator serves as a pulse source. To reduce the insulation distance to the walls of the shielding cabin the Marx generator is operated in ground symmetric configuration. The device has been designed for a batch-wise throughput of 50 kg/h. An automatic feeding device enables the alternating use of two test vessels. The device has been tested on-site on a farm with green rye and grass.
Identification of the gene encoding the major latency-associated nuclear antigen of the Kaposi's sarcoma-associated herpesvirus. (1997)

Over 85% of patients with Kaposi's sarcoma (KS) are seropositive for antibodies to the latency-associated nuclear antigen (LANA) expressed in B cell lines infected with Kaposi's sarcoma-associated herpesvirus (KSHV). The presence of antibodies to LANA strongly correlates with the risk of developing the disease. However, the identity of the protein(s) comprising LANA and the corresponding gene(s) has remained unclear. To identify potential latent gene candidates for LANA, we probed total RNA extracted from BCBL-1 cells (a B cell line latently infected with KSHV) using lambda clones that span the KSHV genome. One region encoding latent transcripts spanned KSHV open reading frames (orfs) 71 (K13), 72 (v-cyclin), and 73. Among these, however, only orf 73, when expressed in heterologous mammalian cell systems, reacted with KSHV antibody-positive human sera, resulting in a punctate nuclear staining pattern reminiscent of LANA in BCBL-1 cells. Furthermore, extracts from cells expressing the orf 73 protein product specifically blocked the binding of KS patient antibodies to LANA. Finally, seroreactivity with recombinant orf 73 protein exactly paralleled reactivity with classical LANA as expressed in BCBL-1 cells, both in KS patients and in other groups. Together, these data support the identification of KSHV orf 73 as the gene encoding the dominant immunogenic component of LANA.
An Environmental Disinfection Odyssey: Evaluation of Sequential Interventions to Improve Disinfection of <i>Clostridium difficile</i> Isolation Rooms (2013)

OBJECTIVE. Effective disinfection of hospital rooms after discharge of patients with Clostridium difficile infection (CDI) is necessary to prevent transmission. We evaluated the impact of sequential cleaning and disinfection interventions by culturing high-touch surfaces in CDI rooms after cleaning. DESIGN. Prospective intervention. SETTING. A Veterans Affairs hospital. INTERVENTIONS. During a 21-month period, 3 sequential tiered interventions were implemented: (1) fluorescent markers to provide monitoring and feedback on thoroughness of cleaning facility-wide, (2) addition of an automated ultraviolet radiation device for adjunctive disinfection of CDI rooms, and (3) enhanced standard disinfection of CDI rooms, including a dedicated daily disinfection team and implementation of a process requiring supervisory assessment and clearance of terminally cleaned CDI rooms. To determine the impact of the interventions, cultures were obtained from CDI rooms after cleaning and disinfection. RESULTS. The fluorescent marker intervention improved the thoroughness of cleaning of high-touch surfaces (from 47% to 81% marker removal; P < .0001). Relative to the baseline period, the prevalence of positive cultures from CDI rooms was reduced by 14% (P=.024), 48% (P <.001), and 89% (P=.006) with interventions 1, 2, and 3, respectively. During the baseline period, 67% of CDI rooms had positive cultures after disinfection, whereas during interventions periods 1, 2, and 3 the percentages of CDI rooms with positive cultures after disinfection were reduced to 57%, 35%, and 7%, respectively. CONCLUSIONS. An intervention that included formation of a dedicated daily disinfection team and implementation of a standardized process for clearing CDI rooms achieved consistent CDI room disinfection. Culturing of CDI rooms provides a valuable tool to drive improvements in environmental disinfection.
Detecting and Recognizing Human-Object Interactions (2018)

To understand the visual world, a machine must not only recognize individual object instances but also how they interact. Humans are often at the center of such interactions and detecting human-object interactions is an important practical and scientific problem. In this paper, we address the task of detecting (human, verb, object) triplets in challenging everyday photos. We propose a novel model that is driven by a human-centric approach. Our hypothesis is that the appearance of a person - their pose, clothing, action - is a powerful cue for localizing the objects they are interacting with. To exploit this cue, our model learns to predict an action-specific density over target object locations based on the appearance of a detected person. Our model also jointly learns to detect people and objects, and by fusing these predictions it efficiently infers interaction triplets in a clean, jointly trained end-to-end system we call InteractNet. We validate our approach on the recently introduced Verbs in COCO (V-COCO) and HICO-DET datasets, where we show quantitatively compelling results.
Gastrointestinal nitric oxide generation in germ-free and conventional rats (2004)

Nitric oxide (NO) is a central mediator of various physiological events in the gastrointestinal tract. The influence of the intestinal microflora for NO production in the gut is unknown. Bacteria could contribute to this production either by stimulating the mucosa to produce NO, or they could generate NO themselves. Using germ-free and conventional rats, we measured gaseous NO directly in the gastrointestinal tract and from the luminal contents using a chemiluminescence technique. Mucosal NO production was studied by using an NO synthase (NOS) inhibitor, and to evaluate microbial contribution to the NO generation, nitrate was given to the animals. In conventional rats, luminal NO differed profoundly along the gastrointestinal tract with the greatest concentrations in the stomach [>4,000 parts per billion (ppb)] and cecum (approximately 200 ppb) and lower concentrations in the small intestine and colon (< or =20 ppb). Cecal NO correlated with the levels in incubated luminal contents. NOS inhibition lowered NO levels in the colon, without affecting NO in the stomach and in the cecum. Gastric NO increased greatly after a nitrate load, proving it to be a substrate for NO generation. In germ-free rats, NO was low (< or =30 ppb) throughout the gastrointestinal tract and absent in the incubated luminal contents. NO also remained low after a nitrate load. Our results demonstrate a pivotal role of the intestinal microflora in gastrointestinal NO generation. Distinctly compartmentalized qualitative and quantitative NO levels in conventional and germ-free rats reflect complex host microbial cross talks, possibly making NO a regulator of the intestinal eco system.
Person Re-identification by Attributes (2012)

Visually identifying a target individual reliably in a crowded environment observed by a distributed camera network is critical to a variety of tasks in managing business information, border control, and crime prevention. Automatic re-identification of a human candidate from public space CCTV video is challenging due to spatiotemporal visual feature variations and strong visual similarity between different people, compounded by low-resolution and poor quality video data. In this work, we propose a novel method for re-identification that learns a selection and weighting of mid-level semantic attributes to describe people. Specifically, the model learns an attribute-centric, parts-based feature representation. This differs from and complements existing low-level features for re-identification that rely purely on bottom-up statistics for feature selection, which are limited in discriminating and identifying reliably visual appearances of target people appearing in different camera views under certain degrees of occlusion due to crowdedness. Our experiments demonstrate the effectiveness of our approach compared to existing feature representations when applied to benchmarking datasets.
Identification of bacteria and bacteria-associated chemical cues that mediate oviposition site preferences by<i>Aedes aegypti</i> (2008)

The yellow fever mosquito, Aedes aegypti, the global vector of dengue and yellow fever, is inexorably linked to water-filled human-made containers for egg laying and production of progeny. Oviposition is stimulated by cues from water containers, but the nature and origin of these cues have not been elucidated. We showed that mosquito females directed most of their eggs to bamboo and white-oak leaf infusions, and only a small fraction of the eggs were laid in plain water containers. In binary choice assays, we demonstrated that microorganisms in leaf infusions produced oviposition-stimulating kairomones, and using a combination of bacterial culturing approaches, bioassay-guided fractionation of bacterial extracts, and chemical analyses, we now demonstrate that specific bacteria-associated carboxylic acids and methyl esters serve as potent oviposition stimulants for gravid Ae. aegypti. Elucidation of these compounds will improve understanding of the chemical basis of egg laying behavior of Ae. aegypti, and the kairomones will likely enhance the efficacy of surveillance and control programs for this disease vector of substantial global public health importance.
Molecular cloning and functional expression of a third isoform of the human calcitonin receptor and partial characterization of the calcitonin receptor gene (1995)

We have cloned and expressed two isoforms of the human calcitonin (hCT) receptor. Primers designed from the published sequence of a CT receptor cloned from an ovarian small cell carcinoma line were used for the polymerase chain reaction amplification of related products from human breast carcinoma MCF-7 cells. Two complementary DNAs were isolated. One clone lacks a 16-amino acid insert in the first intracellular loop and is virtually identical to the receptor recently cloned from the T47D human breast carcinoma cell line. The second clone is another splice variant lacking both the 16-amino acid insert in the first intracellular domain as well as the first 47 amino acids of the amino-terminus extracellular domain. COS-7 cells transfected with either receptor isoform bound [125I]salmon CT with high affinity and responded to hCT with increases in cAMP. Tissue distribution studies revealed the truncated extracellular domain 1 isoform transcripts in human skeletal muscle, kidney, brain, and lung. Analysis of a hCT receptor genomic clone demonstrated an exon/intron organization similar to that of the porcine CT receptor gene, except for a distinct exon coding for the alternatively spliced insert in the first intracellular domain.
Fluoxetine: a randomized clinical trial in the treatment of obesity. (1994)

Fluoxetine hydrochloride (Lovan, Eli Lilly and Company, Indianapolis, Indiana, USA), a specific serotonin uptake inhibitor, was compared with placebo in 458 obese outpatients in a 52-week double-blind randomized ten-site trial to study its effect on weight reduction. Patients in the fluoxetine and placebo groups were predominantly Caucasian (81% and 85%, respectively) and female (81% and 79%, respectively), with a mean body mass index (BMI) of 36.2 and 35.8 kg/m2, respectively, and a mean age of 43 years (both groups). Fluoxetine therapy (60 mg/day) resulted in statistically significantly (P < or = 0.05) greater mean weight loss than placebo to week 28. Although some patients continued to lose weight throughout the 52-week therapy period, maximum mean weight loss occurred at week 20. There was no treatment difference at 52 weeks. The change in visit frequency (biweekly to week 8, monthly to week 20, then bimonthly to week 52) may have affected results. Patients with higher baseline BMIs (> 40 kg/m2) attained and maintained a greater weight loss than patients with lower baseline BMIs (< 40 kg/m2). Two sites demonstrated greater efficacy than the study as a whole. The use of nutrition counselling at one site and behaviour modification at the other, or other site-to-site differences, may account for the improved efficacy. Fluoxetine was well tolerated and appeared to be safe therapy for the treatment of obesity with efficacy demonstrated for 28 weeks.
Plasma glucose measurement with the Yellow Springs Glucose Analyzer. (1978)

The Yellow Springs Glucose Analyzer, a device for the quantitative measurement of glucose concentrations, involves the use of immobilized glucose oxidase, incorporated on a membrane covering a hydrogen peroxide sensor. Operation of the instrument is simple. After an initial calibration, 25 microliter of plasma is injected into a reaction chamber. At 45 s a digital result for glucose is displayed. The within-batch coefficient of variation for the method is 1.2% or less for glucose concentrations of 0.94 to 3.98 g/liter. The between-batch coefficient of variation is 5.8% or less for glucose concentrations of 0.29 to 2.91 g/liter. Concentration and readout are linearly related to at least 4.6 g/liter. Analytical recoveries ranged from 100 to 102%. Carry-over was negligible. Values for glucose concentration obtained with the instrument compared well (r = 0.997) with those obtained with the Beckman Glucose Analyzer.
Leidenfrost Dynamics (2012)

This review discusses how drops can levitate on a cushion of vapor when brought in contact with a hot solid. This is the so-called Leidenfrost phenomenon, a dynamical and transient effect, as vapor is injected below the liquid and pressed by the drop weight. The absence of solid/liquid contact provides unique mobility for the levitating liquid, contrasting with the usual situations in which contact lines induce adhesion and enhanced friction: hence a frictionless motion, and the possibility of bouncing after impact. All these characteristics can be combined to create devices in which self-propulsion is obtained, using asymmetric textures on the hot solid surface.
Cognitive Status and the Form of Referring Expressions in Discourse (1993)

In this paper we propose six implicationally related cognitive statuses relevant for explicating the use of referring expressions in natural language discourse. These statuses are the conventional meanings signalled by determiners and pronouns, and interaction of the statuses with Grice's Maxim of Quantity accounts for the actual distribution and interpretation of forms when necessary conditions for the use of more than one form are met. This proposal is supported by an empirical study of the distribution of referring expressions in naturally occurring discourse in five languages—English, Japanese, Mandarin Chinese, Russian, and Spanish.
ChemInform Abstract: Palladium‐Catalyzed Oxidative Cyclization of N‐Aryl Enamines: From Anilines to Indoles. (2008)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Cell-specific post-translational processing of preproglucagon expressed from a metallothionein-glucagon fusion gene. (1986)

Glucagon is a peptide hormone of 29 amino acids encoded by a preprohormone which contains in tandem the sequences of glucagon and two additional glucagon-like peptides (GLPs) structurally related to glucagon and separated by intervening peptides. Glucagon arises by cleavage from the prohormone within the A cells of the pancreatic islets but in the intestine remains as part of a partially processed precursor (glicentin). To determine whether additional glucagon-like peptides are processed from preproglucagon and to analyze for potential cellular specificity in the processing of preproglucagon, we introduced and expressed a metallothionein-glucagon fusion gene in a fibroblast and two endocrine (pituitary and pancreatic islet) cell lines. Chromatographic analyses of cell extracts utilizing specific radioimmunoassays to chemically synthesized peptides demonstrate the liberation of intact glucagon, glicentin, GLP-I(1-37), GLP-I(7-37), GLP-II, and an intervening peptide amidated at its carboxyl terminus. The peptides were present in distinct yet different patterns in the two endocrine but not the fibroblast cell lines. The cell-specific liberation of the glucagon-like and intervening peptides suggests their potential as new bioactive peptides. The cellular specificity in the processing of preproglucagon indicates that the genetic determinants of the processing activity are complex and are expressed in a cell-specific manner.