gebhart/patrepeval huggingface dataset:
Autoadaptive phase‐correlated (AAPC) reconstruction for 4D CBCT (2009)

The method reduces the noise in the reconstruction and increases the image quality. More data are introduced for each phase-correlated reconstruction, and therefore the applied dose is used more efficiently.
<scp><i>Saccharomyces cerevisiae</i></scp> Shuttle vectors (2017)

Yeast shuttle vectors are indispensable tools in yeast research. They enable cloning of defined DNA sequences in Escherichia coli and their direct transfer into Saccharomyces cerevisiae cells. There are three types of commonly used yeast shuttle vectors: centromeric plasmids, episomal plasmids and integrating plasmids. In this review, we discuss the different plasmid systems and their characteristic features. We focus on their segregational stability and copy number and indicate how to modify these properties. Copyright © 2017 John Wiley & Sons, Ltd.
Identification of Genes Critical for Resistance to Infection by West Nile Virus Using RNA-Seq Analysis (2013)

The West Nile virus (WNV) is an emerging infection of biodefense concern and there are no available treatments or vaccines. Here we used a high-throughput method based on a novel gene expression analysis, RNA-Seq, to give a global picture of differential gene expression by primary human macrophages of 10 healthy donors infected in vitro with WNV. From a total of 28 million reads per sample, we identified 1,514 transcripts that were differentially expressed after infection. Both predicted and novel gene changes were detected, as were gene isoforms, and while many of the genes were expressed by all donors, some were unique. Knock-down of genes not previously known to be associated with WNV resistance identified their critical role in control of viral infection. Our study distinguishes both common gene pathways as well as novel cellular responses. Such analyses will be valuable for translational studies of susceptible and resistant individuals--and for targeting therapeutics--in multiple biological settings.
Isolation of O-demethyllycoramine from bulbs of Lycoris radiata Herb. (1980)

The bulbs of Lycoris radiata HERB. (Amaryllidaceae) were found to contain a new phenolic base, O-demethyllycoramine (O-demethyldihydrogalanthamine) (3), as well as previously isolated alkaloids, such as pretazettine (1), lycorine (4), lycoramine (5), lycorenine (6), demethylhomolycorine (7), hippeastrine (8), and homolycorine (9). The structure of 3 was confirmed by demethylation of lycoramine (5) with pyridine hydrochloride.
Loss of the N-myc oncogene in a patient with a small interstitial deletion of the short arm of chromosome 2 (1996)

To our knowledge, only four previous cases of distal chromosome 2p deletions exist in the literature. We present a patient with minor facial anomalies who had a distal interstitial deletion of the short arm of chromosome 2, del(2)(p24.2p25.1). This patient had many features seen in other patients with distal 2p deletion including short stature, "rectangular" facies, microcephaly, hypotonia, and mental retardation. This patient also has sensorineural hearing loss which has been described in one other patient with a similar deletion. The N-myc oncogene has been mapped to 2p24. By fluorescence in situ hybridization using a cDNA probe for the N-myc oncogene, this patient was found to have a deletion of the N-myc oncogene. This confirms the previous map location for N-myc.
Correcting the expression of miRNA-155 represses PP2Ac and enhances the release of IL-2 in PBMCs of juvenile SLE patients (2014)

MicroRNA-155 is involved in immune cell, differentiation, maturation and function. MiR-155 showed variable dysregulated expression in autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) patients. MiR-155 was previously confirmed to directly target CAMP response element binding protein (CREB), which was previously identified as a positive regulator of protein phosphatase 2A (PP2A). PP2A is a key negative regulator of interleukin-2, which is an important immune modulator and was previously shown to be decreased in SLE. In this study we aimed at investigating the regulation of PP2A by miR-155 and hence its role in juvenile SLE disease pathogenesis. MiR-155 showed significant downregulation in PBMCs from juvenile SLE and juvenile familial Mediterranean fever (FMF) and significant upregulation in PBMCs from juvenile idiopathic arthritis (JIA) patients. In SLE, miR-155 expression was negatively correlated with Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score and proteinuria and was positively correlated with white blood cell (WBC) count. The mRNA of the catalytic subunit of PP2A (PP2Ac) showed significant upregulation in PBMCs from SLE and FMF but not in JIA patients. Additionally, the relative expression of PP2Ac mRNA was positively correlated with SLEDAI score. Forced expression of miR-155 led to decreased relative expression of PP2Ac mRNA and increased IL-2 release in cultured-stimulated PBMCs. This study suggests for the first time the possible role of an miR-155-PP2Ac loop in regulating IL-2 release and identifies miR-155 as a potential therapeutic target in juvenile SLE disease through relieving IL-2 from the inhibitory role of PP2A.
Pigment Encapsulation by Emulsion Polymerization Using Macro-RAFT Copolymers (2008)

A new method is described, based on living amphipathic random macro-RAFT copolymers, which enables the efficient polymeric encapsulation of both inorganic and organic particulate materials via free-radical polymerization. The mechanism for this new approach is examined in the context of the polymer coating of zirconia- and alumina-coated titanium dioxide particles and its breadth of application demonstrated by the coating of organic phthalocyanine blue pigment particles. The particulate materials were first dispersed in water using a macro-RAFT copolymer as a stabilizer. Monomer and water-soluble initiator were then added to the system, and the monomer polymerized to form the coating. If nucleation of new polymer particles in the aqueous phase was to be avoided, it was found necessary to use a macro-RAFT copolymer that did not form micelles; within this constraint, a broad range of RAFT agents could be used. The macro-RAFT agents used in this work were found not to transfer competitively in the aqueous phase and therefore did not support growth of aqueous-phase polymer. Successful encapsulation of particles was demonstrated by TEM. The process described enables 100% of the particles to be encapsulated with greater than 95% of the polymer finishing up in the polymeric shells around the particles. Moreover, the coating reaction can be carried out at greater than 50% solids in many cases and avoids the agglomeration of particles during the coating step.
Increased Expression of Estrogen Receptor-β mRNA in Male Blood Vessels After Vascular Injury (1998)

Estrogen exerts direct effects on vascular endothelial and smooth muscle cells that are important for vascular protection. Estrogen receptor-alpha (ERalpha) is expressed in vascular cells from males and females and may mediate some of the effects of estrogen on vascular tissue. However, we recently found that estrogen is able to protect against vascular injury in ovariectomized female ERalpha knockout mice. These mice express the newly described estrogen receptor-beta (ERbeta) in their aortas, suggesting that ERbeta may also mediate some of the direct effects of estrogen on the vasculature. In this study, the level of expression of ERalpha and ERbeta mRNA in male rat aortas was examined before and after vascular injury using en face (Häutchen) preparations and in situ hybridization. Little or no change in ERalpha expression was observed after vascular injury in either vascular endothelial or smooth muscle cells at any time point. In contrast, ERbeta mRNA was found to be expressed markedly after balloon injury. In endothelial cells, ERbeta was increased by 2 days after injury, and high levels of expression were maintained at 8 and 14 days. Furthermore, ERbeta expression was high in luminal smooth muscle cells at 8 and 14 days after injury and had decreased to low levels by 28 days after injury. These data demonstrate the presence of ERbeta in male vascular tissues and the induction of ERbeta mRNA expression after vascular injury, supporting a role for ERbeta in the direct vascular effects of estrogen.
Persistent B <sup>+</sup> -trees in non-volatile main memory (2015)

Computer systems in the near future are expected to have &lt;u&gt;N&lt;/u&gt;on-&lt;u&gt;V&lt;/u&gt;olatile &lt;u&gt;M&lt;/u&gt;ain &lt;u&gt;M&lt;/u&gt;emory (NVMM), enabled by a new generation of &lt;u&gt;N&lt;/u&gt;on-&lt;u&gt;V&lt;/u&gt;olatile &lt;u&gt;M&lt;/u&gt;emory (NVM) technologies, such as Phase Change Memory (PCM), STT-MRAM, and Memristor. The non-volatility property has the promise to persist in-memory data structures for instantaneous failure recovery. However, realizing such promise requires a careful design to ensure that in-memory data structures are in known consistent states after failures. This paper studies persistent in-memory B + -Trees as B + -Trees are widely used in database and data-intensive systems. While traditional techniques, such as undo-redo logging and shadowing, support persistent B + -Trees, we find that they incur drastic performance overhead because of extensive NVM writes and CPU cache flush operations. PCM-friendly B + -Trees with unsorted leaf nodes help mediate this issue, but the remaining overhead is still large. In this paper, we propose write atomic B + -Trees (wB + -Trees), a new type of main-memory B + -Trees, that aim to reduce such overhead as much as possible. wB + -Tree nodes employ a small indirect slot array and/or a bitmap so that most insertions and deletions do not require the movement of index entries. In this way, wB + -Trees can achieve node consistency either through atomic writes in the nodes or by redo-only logging. We model fast NVM using DRAM on a real machine and model PCM using a cycle-accurate simulator. Experimental results show that compared with previous persistent B + -Tree solutions, wB + -Trees achieve up to 8.8x speedups on DRAM-like fast NVM and up to 27.1x speedups on PCM for insertions and deletions while maintaining good search performance. Moreover, we replaced Memcached's internal hash index with tree indices. Our real machine Memcached experiments show that wB + -Trees achieve up to 3.8X improvements over previous persistent tree structures with undo-redo logging or shadowing.
The total synthesis of cystodytins (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe total synthesis of cystodytinsMarco A. Ciufolini and Norman E. ByrneCite this: J. Am. Chem. Soc. 1991, 113, 21, 8016–8024Publication Date (Print):October 1, 1991Publication History Published online1 May 2002Published inissue 1 October 1991https://pubs.acs.org/doi/10.1021/ja00021a031https://doi.org/10.1021/ja00021a031research-articleACS PublicationsRequest reuse permissionsArticle Views816Altmetric-Citations52LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Improved Vascular Endothelial Function After Oral B Vitamins (2000)

Folic acid and vitamin B(12) supplementation improves vascular endothelial function in patients with CHD, and this effect is likely to be mediated through reduced concentrations of free plasma homocysteine concentrations. Our data support the view that lowering homocysteine, through B vitamin supplementation, may reduce cardiovascular risk.
Spatially-Coupled MacKay-Neal Codes and Hsu-Anastasopoulos Codes (2011)

Kudekar et al. recently proved that for transmission over the binary erasure channel (BEC), spatial coupling of LDPC codes increases the BP threshold of the coupled ensemble to the MAP threshold of the underlying LDPC codes. One major drawback of the capacity-achieving spatially-coupled LDPC codes is that one needs to increase the column and row weight of parity-check matrices of the underlying LDPC codes. It is proved, that Hsu-Anastasopoulos (HA) codes and MacKay-Neal (MN) codes achieve the capacity of memoryless binary-input symmetric-output channels under MAP decoding with bounded column and row weight of the parity-check matrices. The HA codes and the MN codes are dual codes each other. The aim of this paper is to present an empirical evidence that spatially-coupled MN (resp. HA) codes with bounded column and row weight achieve the capacity of the BEC. To this end, we introduce a spatial coupling scheme of MN (resp. HA) codes. By density evolution analysis, we will show that the resulting spatially-coupled MN (resp. HA) codes have the BP threshold close to the Shannon limit.
원저 : 온구기의 연소 특성에 관한 고찰 (2012)

목적 : 뜸 연소 실험을 통해 간접구의 일종인 온구기의 연소 특성을 조사하였다. 방법 : 온구기를 유리판 위에 놓고 연소하여 50분간 온도 데이터를 수집하였다. 총 20개의 뜸봉을 연소하여 수집된 데이터를 분석하여 평균 온도, 최고 온도, 최고 온도 도달 시간, 최고 온도 지속 시간을 계산하였다. 또 혈류량을 증가시킨다고 보고된 온도(38℃)에 도달하는 시간, 그 이상의 온도로 지속되는 시간, C-fiber의 활성화를 통해 치료효과를 나타내는 것으로 보고된 온도(42-50℃)에 도달하는 시간과 그 범위 내의 지속시간을 계산하였다. 결과 : 평균 최고온도는 50.4℃이고 평균 도달시간은 1,007초, 평균 지속시간은 27초였다. 평균최고 온도는 보고된 다른 간접구와 유사하였으나 평균 도달시간은 더 길었다. 38℃에 도달하는 평균시간은 410초이고 지속시간은 1,813초였다. 42-50℃에 도달하는 평균시간은 521초이고 지속시간은 990초였다. 결론 : 온구기는 연소 특성이 보고된 다른 종류의 뜸보다 더 오랜 시간 동안 안정적인 열자극이 가능하여, 뜸치료의 임상 활용의 폭을 높일 수 있을 것으로 사료된다.
Dimethyl carbonate production for fuel additives (1996)

We have taken note of the transesterification reaction as a highly safe process of dimethyl carbonate (DMC) production for fuel additives. The reaction proceeds under the low corrosiveness and in the relatively mild condition. We have aimed to use an inorganic solid catalyst for this process. The inorganic solid catalyst is thermally stable and can be used in the large-scale fixed bed reactors without a catalyst separation unit. Through the transesterification of ethylene carbonate (EG) with methanol, DMC and ethylene glycol (EG) are co-generated as the products. EG is one of the bulk chemicals produced in the large scale plant comparable to one for the fuel additives. The market balance is important in the coproduction process. On the assumption that the amount of the co-production meets the market balance, the coproduction of DMC and EG is commercially viable. If we can control the amount of the EG coproduction in this process, it makes the process more flexible in the commercial production. Accordingly we have proposed a conceptual process scheme to control the amount of the EG coproduction. In this symposium, the inorganic solid catalyst system applying to the transesterification process and the conceptual process scheme how to control the amountmore » of co-product will be discussed.« less
Service location protocol: automatic discovery of IP network services (1999)

As long as configuration remains difficult, network administration will be expensive, tedious, and troublesome and users-especially mobile users-will be unable to take full advantage of network services. SLP is an IETF Proposed Standard for enabling network-based service discovery and automatic configuration of clients. It provides for fully decentralized operation and scales from a small, unadministered network up to an enterprise network. The essential function of SLP is service discovery, but it has also been designed to support security extensibility, browsing operations, and operation over IPv6. The article describes SLP's operation.
&lt;title&gt;Status of BMDO/IST lasercom advanced technology demonstration&lt;/title&gt; (1994)

Laser communications between satellites, high flying aircraft (such as JSTARS), and the ground offer the potential to transfer extremely high amounts of information faster and with a much smaller package than is possible using current radio frequency and microwave technologies. This can be especially important in downlinking time sensitive satellite reconnaissance information because the satellite stays within range of a ground station or aircraft for only a few minutes. A capability to downlink from a satellite to an aircraft can provide all weather performance, and multiple data transfers for every satellite orbit. Over the last few years, SDIO (now BMDO) has funded a number of technology efforts through the US Army Space and Strategic Defense Command reducing the risks associated with laser communications. This paper describes one of these efforts which is now being carried forward to an Advanced Technology Demonstration at ThermoTrex Corporation. The program will lead to the demonstration of high data rate communications of 270 MBPS (Mega Bits Per Second) to 1.08 GBPS (Giga Bits Per Second) between high altitude aircraft and possibly between a satellite and the ground. The Laser Communications Terminals incorporate Atomic Line Filter technology for background light rejection during acquisition, reactionless Roto-Lok offset cable drive gimbals for fast slewing and high accuracy pointing, and direct digital modulation of semiconductor diode lasers detected with low noise avalanche photodiodes. We present designs and preliminary performance results for both a simplified terminal appropriate for a near term satellite-to-ground data transfer experiment, and a full capability terminal appropriate for ground, aircraft, or satellite operations.
Interdigitated electrodes as impedance and capacitance biosensors: A review (2017)

Interdigitated electrodes (IDEs) are made of two individually addressable interdigitated comb-like electrode structures. IDEs are one of the most favored transducers, widely utilized in technological applications especially in the field of biological and chemical sensors due to their inexpensive, ease of fabrication process and high sensitivity. In order to detect and analyze a biochemical molecule or analyte, the impedance and capacitance signal need to be obtained. This paper investigates the working principle and influencer of the impedance and capacitance biosensors. The impedance biosensor depends on the resistance and capacitance while the capacitance biosensor influenced by the dielectric permittivity. However, the geometry and structures of the interdigitated electrodes affect both impedance and capacitance biosensor. The details have been discussed in this paper.
Naloxone acts as an antagonist of estrogen receptor activity in MCF-7 cells (2006)

Estrogen promotes the growth of breast cancer via estrogen receptors (ER). Earlier studies showed that the opioid receptor antagonist naloxone inhibits MCF-7 breast cancer growth in mice. We examined the cellular and molecular mechanism of naloxone antagonism of ERalpha activity in human MCF-7 cells. Naloxone (100 nmol/L) inhibited 17beta-estradiol (E2)-induced (10 nmol/L) MCF-7 cell proliferation by 65% and mitogen-activated protein kinase/extracellular signal-regulated kinase phosphorylation. Naloxone blocked the E2-induced activation of ERalpha, with 85% inhibition after 5 minutes and 100% recovery after 60 minutes. This assay is based on quantitation of E2-activated nuclear ERalpha binding to the immobilized coactivator peptide. A significant decrease in E2-induced ERalpha transactivation was observed in the presence of naloxone in the estrogen response element-luciferase reporter assay (P < 0.05, E2 versus E2 + naloxone). Naloxone also blocked E2-induced down-regulation of ERalpha mRNA at 30 minutes and 6 hours. Although naloxone inhibits ERalpha activity directly, it also induces a cross-talk between mu-opioid receptor (MOR) and ERalpha. Immunoprecipitates with anti-MOR antibody showed the presence of ERalpha in cells incubated with E2 in the presence of naloxone but not in cells incubated with E2 or naloxone alone. Higher amounts of ERalpha associated with MOR after 60 minutes compared with 10 minutes of incubation. Naloxone inhibited E2-bovine serum albumin-FITC binding to plasma membrane-associated ERalpha and also inhibited the direct binding of [3H]E2 to ERalpha. Thus, naloxone modulates ERalpha activity directly as well as indirectly via MOR. This study suggests that naloxone-like compounds can be developed as novel therapeutic molecules for breast cancer therapy.
Expression of TIMP3 mRNA is elevated in retinas affected by simplex retinitis pigmentosa (1994)

To explore the molecular and cellular mechanisms associated with photoreceptor death in retinitis pigmentosa (RP), we have investigated altered transcriptional activity in RP retinas by a differential cDNA screening approach. We identified a clone (K222) showing over-expression in simplex RP retinas compared with controls. K222 encodes a partial cDNA of the human tissue inhibitor of metalloproteinases-3 (TIMP3) gene, a member of a family of genes implicated in extracellular matrix (ECM) remodelling. Increased expression of TIMP3 in degenerating RP retinas may reflect restructuring of the ECM architecture, and disruption of photoreceptor-matrix interactions could contribute to activation of apoptotic cell death processes.
Large scale self-assembly of crystalline semiconductor microcomponents onto plastic substrates via microfluidic traps (2008)

We present high-yield self-assembly results of silicon microcomponents assembled onto plastic substrates. The self-assembly is achieved by engineering the fluid flow over the substrate containing an array of microfluidic traps. This simple self-assembly method has demonstrated physical yields exceeding 90% as well as electrical connections between the microcomponents and the substrate. Using templates with 1250 binding sites, we were able to study the statistical nature of the self-assembly process experimentally and determine two exponential spatial and temporal trends affecting the overall yield.
Isolation of unique sequence human X chromosomal deoxyribonucleic acid (1980)

Labeled unique sequence human X chromosomal DNA has been isolated by hybridization of labeled human DNA with DNA from a human-mouse hybrid cell line, A9/HRBC2-A, which contains a single human chromosome, the X. Homopolymer tails of poly(dA) were added to nick-translated unique sequence human DNA to permit separation of the labeled DNA from unlabeled driver DNA by binding to oligo(dT)-cellulose. Human DNA sequences homologous with mouse DNA were removed from this labeled poly-(dA)-tailed human probe by hybridization with excess unlabeled mouse DNA. The labeled human DNA which did not hybridize with mouse DNA was then hybridized with excess unlabeled A9/HRBC2-A DNA, with which only X chromosomal human DNA will hybridize. After hybridization, the labeled human X chromosomal DNA was separated from the unlabeled A9/HRBC2-A DNA by binding to oligo(dT)-cellulose. The purity of the final X chromosomal DNA preparation was greater than 90%, and the hybridization with mouse DNA was less than 2%. When carried out under standard conditions for DNA reassociation, this procedure is complicated by the formation of hybrids between the poly(dA) tails of the probe and T-rich sequences in mouse and human DNA. However, these imperfectly paired hybrids are less stable than those of unique sequence DNA and can be eliminated by carrying out the hydroxylapatite chromatography at an elevated temperature of 71 degrees C.