gebhart/patrepeval huggingface dataset:
The Partial Hydrolysis of Methyltrimethoxysilane (1955)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Partial Hydrolysis of MethyltrimethoxysilaneM. M. Sprung and F. O. GuentherCite this: J. Am. Chem. Soc. 1955, 77, 15, 4173–4175Publication Date (Print):August 1, 1955Publication History Published online1 May 2002Published inissue 1 August 1955https://pubs.acs.org/doi/10.1021/ja01620a070https://doi.org/10.1021/ja01620a070research-articleACS PublicationsRequest reuse permissionsArticle Views241Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Glutamine Synthetase Is a Genetic Determinant of Cell Type–Specific Glutamine Independence in Breast Epithelia (2011)

Although significant variations in the metabolic profiles exist among different cells, little is understood in terms of genetic regulations of such cell type-specific metabolic phenotypes and nutrient requirements. While many cancer cells depend on exogenous glutamine for survival to justify the therapeutic targeting of glutamine metabolism, the mechanisms of glutamine dependence and likely response and resistance of such glutamine-targeting strategies among cancers are largely unknown. In this study, we have found a systematic variation in the glutamine dependence among breast tumor subtypes associated with mammary differentiation: basal- but not luminal-type breast cells are more glutamine-dependent and may be susceptible to glutamine-targeting therapeutics. Glutamine independence of luminal-type cells is associated mechanistically with lineage-specific expression of glutamine synthetase (GS). Luminal cells can also rescue basal cells in co-culture without glutamine, indicating a potential for glutamine symbiosis within breast ducts. The luminal-specific expression of GS is directly induced by GATA3 and represses glutaminase expression. Such distinct glutamine dependency and metabolic symbiosis is coupled with the acquisition of the GS and glutamine independence during the mammary differentiation program. Understanding the genetic circuitry governing distinct metabolic patterns is relevant to many symbiotic relationships among different cells and organisms. In addition, the ability of GS to predict patterns of glutamine metabolism and dependency among tumors is also crucial in the rational design and application of glutamine and other metabolic pathway targeted therapies.
A Dual Target-directed Agent against Interleukin-6 Receptor and Tumor Necrosis Factor α ameliorates experimental arthritis (2016)

A considerable proportion of patients with rheumatoid arthritis (RA) do not respond to monospecific agents. The purpose of our study was to generate a hybrid form of biologics, targeting tumor-necrosis factor alpha (TNFα) and interleukin-6 receptor (IL-6R), and determine its anti-arthritic properties in vitro and in vivo. A novel dual target-directed agent (DTA(A7/sTNFR2)) was generated by conjugating soluble TNF receptor 2 (sTNFR2) to the Fc region of A7, a new anti-IL-6R antibody obtained by screening the phage display human antibody library. DTA(A7/sTNFR2) inhibited the proliferation and migration of fibroblast-like synoviocytes from patients with RA (RA-FLS) more efficiently than single target-directed agents. DTA(A7/sTNFR2) also blocked osteoclastogenesis from bone marrow cells. The arthritis severity scores of the experimental arthritis mice with DTA(A7/sTNFR2) tended to be lower than those of mice with IgG, A7, or sTNFR2. Histological data suggested that DTA(A7/sTNFR2) is more efficient than single-target drugs in preventing joint destruction and bone loss. These results were confirmed in vivo using the minicircle system. Taken together, the results show that DTA(A7/sTNFR2) may be a promising therapeutic agent for the treatment of RA.
Severe Impairment in Early Host Defense against<i>Candida albicans</i>in Mice Deficient in Myeloperoxidase (1999)

Myeloperoxidase (MPO) catalyzes the reaction of hydrogen peroxide with chloride ion to produce hypochlorous acid (HOCl), which is used for microbial killing by phagocytic cells. Despite the important role of MPO in host defense, however, MPO deficiency is relatively common in humans, and most of these individuals are in good health. To define the in vivo role of MPO, we have generated by gene targeting mice having no MPO activity in their neutrophils and monocytes. The mice without MPO developed normally, were fertile, and showed normal clearance of intraperitoneal Staphylococcus aureus. However, they showed increased susceptibility to pneumonia and death following intratracheal infection with Candida albicans. Furthermore, the lack of MPO significantly enhanced the dissemination of intraperitoneally injected C. albicans into various organs during the first 7 days. Thus, MPO is important for early host defense against fungal infection, and the inability to generate HOCl cannot be compensated for by other oxygen-dependent systems in vivo in mice. The mutant mice serve as a model for studying pulmonary and systemic candidiasis.
Constitutive and Inducible Green Fluorescent Protein Expression in<i>Bartonella henselae</i> (1998)

The green fluorescent protein (GFP) gene was expressed on a plasmid in B. henselae, and GFP-expressing bacteria were visualized by fluorescence microscopy. HEp-2 cells infected with GFP-expressing bacteria were separated from uninfected cells with a fluorescence activated cell sorter. Promoter fusions of B. henselae chromosomal DNA to gfp were examined by flow cytometry, and a B. henselae groEL promoter fusion which induced expression at 37 degreesC was isolated.
Myocarditis, Pericarditis, and Dilated Cardiomyopathy after Smallpox Vaccination among Civilians in the United States, January–October 2003 (2008)

Myocarditis was reported after smallpox vaccination in Europe and Australia, but no association had been reported with the US vaccine. We conducted surveillance to describe and determine the frequency of myocarditis and/or pericarditis (myo/pericarditis) among civilians vaccinated during the US smallpox vaccination program between January and October 2003. We developed surveillance case definitions for myocarditis, pericarditis, and dilated cardiomyopathy after smallpox vaccination. We identified 21 myo/pericarditis cases among 37,901 vaccinees (5.5 per 10,000); 18 (86%) were revacinees, 14 (67%) were women, and the median age was 48 years (range, 25-70 years). The median time from vaccination to onset of symptoms was 11 days (range, 2-42 days). Myo/pericarditis severity was mild, with no fatalities, although 9 patients (43%) were hospitalized. Three additional vaccinees were found to have dilated cardiomyopathy, recognized within 3 months after vaccination. We describe an association between smallpox vaccination, using the US vaccinia strain, and myo/pericarditis among civilians.
Which Way Am I Facing: Inferring Horizontal Device Orientation from an Accelerometer Signal (2009)

We present a method to infer the orientation of mobile device carried in a pocket from the acceleration signal acquired when the user is walking. Whereas previous work has shown how to determine the the orientation in the vertical plane (angle towards earth gravity), we demonstrate how to compute the orientation within the horizontal plane. To validate our method we compare the output of our method with GPS heading information when walking in a straight line. On a total of 16 different orientations and traces we have a mean difference of 5 degrees with 2.5 degrees standard deviation.
Nurturing Neighbourhoods: Parent Outreach Worker Program, Guelph, Ontario. Evaluation Report: November 2014 (2014)

The "Parent Outreach Worker Program" (POW program) aims to identify, reach out to, and assist families with children who may be isolated or otherwise struggling by providing them with increased access to services and support. The POW program is currently being piloted in two priority neighbourhoods of Guelph, Ontario. The POW program is part of a cross-sectoral, community-based "Nurturing Neighbourhoods" initiative that is grounded in the philosophies and principles related to the social determinants of health, health equity, and early help in child welfare. This report details the findings of an evaluation that was conducted in 2013-2014 to determine whether the POW program is being delivered as planned and the extent to which it is achieving its intended outcomes. Evidence used for the evaluation include program implementation data inputted by the Parent Outreach Workers (POWs) and a series of interviews and focus groups conducted with program stakeholders including the POWs, parents who use the POW program, and a variety of associated service providers and community resource people. This early evaluation evidence indicates clearly that the POW program is generating very significant outcomes for priority families as direct supports and as intermediaries between those families and the community resources they need. The POWs occupy an important space in marginalized communities, the evaluation evidence shows, between formal services, informal community supports, and the families most in need of support. Also emerging from the evaluation data are some key "Impact Pathways" through which the POW program is generating results. These Impact Pathways reflect many of the strategies found to be effective in research literature on other outreach programs, and as such may serve as a best practices framework for the establishment of similar programs in other neighbourhoods.
Studies on the Reversible Aggregation of Cysteine-Capped Colloidal Silver Particles Interconnected via Hydrogen Bonds (2001)

The surface modification of aqueous silver colloidal particles with the amino acid cysteine and the cross-linking of the colloidal particles in solution is described. Capping of the silver particles with cysteine is accomplished by a thiolate bond between the amino acid and the nanoparticle surface. The silver colloidal particles are stabilized electrostatically by ionizing the carboxylic acid groups of cysteine. Aging of the cysteine-capped colloidal solution leads to aggregation of the particles via hydrogen bond formation between amino acid molecules located on neighboring silver particles. The aggregation is reversible upon heating the solution above 60 °C. The rate of cross-linking of the silver particles via hydrogen bond formation may be accelerated by screening the repulsive electrostatic interactions between the particles using salt. The process of aggregation and heat-induced dispersion of the particles has been studied by UV−vis spectroscopy, laser light scattering, and transmission electron microscopy measurements.
The Sucrose Transporter of Celery. Identification and Expression during Salt Stress (2000)

In celery (Apium graveolens L.), long-distance transport of reduced carbon occurs both in the form of sucrose (Suc) and mannitol. The presence of mannitol has been related to the resistance of celery to salt stress. To investigate the transport events occurring during salt stress, we have cloned the H(+)/Suc transporter of celery AgSUT1 (A. graveolens Suc uptake transport 1) from a mature leaf cDNA library. The function of the encoded protein was confirmed by expression in yeast. AgSUT1 is a H(+)/Suc transporter with a high affinity for Suc (K(m) of 139 microM). Another closely related cDNA (AgSUT2) was also identified. AgSUT1 is mainly expressed in mature leaves and phloem of petioles, but also in sink organs such as roots. When celery plants were subjected to salt stress conditions (30 d watering with 300 mM NaCl) favoring mannitol accumulation (J.D. Everard, R. Gucci, S.C. Kann, J.A. Flore, W.H. Loescher [1994] Plant Physiol 106: 281-292), AgSUT1 expression was decreased in all organs, but markedly in roots. The results are discussed in relation to the physiology of celery.
Effects of Aging on Neurogenic Vasodilator Responses Evoked by Transcutaneous Electrical Nerve Stimulation: Relevance to Wound Healing (2000)

We have previously shown an age-related decline in the modulation of skin vascular reactivity by sensory nerves that correlates with a decline in wound repair efficacy. This study was designed to examine the possibility that improving the functional ability of aged sensory nerves using noninvasive transcutaneous electrical nerve stimulation (TENS) could also accelerate tissue repair. TENS of the sciatic nerve, combined with measuring blood flow responses in the rat hind-footpad using laser Doppler flowmetry, was used to establish the vascular effects. Following TENS (using parameters 20V, 5 Hz for 1 min), similar increases in vascular responses were obtained in both young (13.2+/-0.9 cm2) and old rats (11.6+/-2.3 cm2). In contrast, capsaicin-pretreated rats showed markedly diminished responses. Sympathetic fibers did not appear to modulate these sensory nerve responses. In the second part, a thermal wound was induced (using a CO2 laser) in the interscapular region of old rats (under anesthesia). In the active treatment group, TENS was applied twice daily for the initial 5 days, and the sham group received inactive TENS. Using the healing endpoint as the time when full wound contraction occurred, the active group required 14.7+/-0.2 days for complete healing, a significant improvement over the sham group (21.8+/-0.3 days). We contend that low-frequency TENS can improve the vascular response of old rats. In addition, wound healing in aged rats can be accelerated by peripheral activation of sensory nerves at low-frequency electrical stimulation parameters.
The mechanism of aluminum-independent G-protein activation by fluoride and magnesium. 31P NMR spectroscopy and fluorescence kinetic studies. (1993)

With magnesium present, fluoride and aluminum ions activate heterotrimeric G-proteins by forming AlFx complexes that mimic the gamma phosphate of a GTP. We report compelling evidence for a newly proposed process of G-protein activation by fluoride and magnesium, without Al3+. With millimolar Mg2+ and F-, Gs and Gt activate adenylylcyclase and cGMP-phosphodiesterase, respectively. In 31P NMR, addition of magnesium to Gi1 alpha GDP or Gt alpha GDP solutions containing fluoride, but no Al3+, modifies the chemical shift of the GDP beta phosphorus, suggesting that magnesium interacts with the beta phosphate. Titration of this effect indicates that two Mg2+ are bound per G alpha. Biphasic activation kinetics, monitored by G alpha tryptophan fluorescence, suggests the rapid binding of one Mg2+ to G alpha GDP and the slow association of another Mg2+, in correlation with fluoride binding and G alpha activation. The deactivation rate upon fluoride dilution shows a second order dependence with respect to the residual F- concentration, suggesting the sequential release of at least three F-/G alpha. Thus, in millimolar Mg2+ and F-, and without Al3+, two Mg2+ and three F- bind sequentially to G alpha GDP and induce the switch to an active G alpha (GDP-MgF3)Mg state, which is structurally analogous to G alpha (GDP-AlFx)Mg and to G alpha (GTP)Mg.
Should nurses be allowed to request X-rays in an accident &amp; emergency department? (1992)

A prospective study was carried out during the month of November, 1990 in the A&E Department, St John's Hospital, Livingston in order to assess the extended role of the A&E nurse and their ability to request X-rays prior to patients being seen by a doctor. A total of 579 randomly selected patients were triaged by A&E Department nurses. Almost 3/4 of these patients were X-rayed at the request of the triage nurse. Less than 7% of these X-rays were considered to have been unnecessary by the doctor who subsequently managed the patient. Of those patients who had an X-ray after seeing a doctor, more than 90% fell within the X-ray triage criteria but had not had an X-ray requested by the triage nurse. Overall, nurses were shown to request X-rays correctly and efficiently with the result that patients had to spend less time in the A&E Department.
Optimal flow control of multiclass queueing networks with partial information (1990)

Structural results and explicit solutions for the optimal flow control problem of multiclass queueing networks with decentralized information are given. Two criteria are investigated: the network (respectively, user) optimization criterion maximizes the average network (user) throughput subject to an average network (user) time delay constraint. It is shown that these problems can be analyzed in terms of an equivalent network by using the generalized Norton's equivalent. The structure of the network (user) optimization problem is exploited to obtain further structural results, namely a representation (separation) theorem. The optimal flow control under both criteria is solved using a linear programming formulation. The structure of the optimal control is shown to be of a window type in both cases. For load-balanced networks, the optimal flow control is found explicitly in terms of the given system parameters.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Advances in therapeutic CRISPR/Cas9 genome editing (2015)

Targeted nucleases are widely used as tools for genome editing. Two years ago the clustered regularly interspaced short palindromic repeat (CRISPR)-associated Cas9 nuclease was used for the first time, and since then has largely revolutionized the field. The tremendous success of the CRISPR/Cas9 genome editing tool is powered by the ease design principle of the guide RNA that targets Cas9 to the desired DNA locus, and by the high specificity and efficiency of CRISPR/Cas9-generated DNA breaks. Several studies recently used CRISPR/Cas9 to successfully modulate disease-causing alleles in vivo in animal models and ex vivo in somatic and induced pluripotent stem cells, raising hope for therapeutic genome editing in the clinics. In this review, we will summarize and discuss such preclinical CRISPR/Cas9 gene therapy reports.
Milk Spoilage: Methods and Practices of Detecting Milk Quality (2013)

Milk spoilage is an indefinite term and difficult to measure with accuracy. This uncertainty can cause suffering for both milk manufacturers and consumers. Consumers who have been misled by ambiguous expiration dates on milk cartons waste resources by disposing of unspoiled milk or experience discomfort from drinking spoiled milk. Consumers are often unwilling to purchase products close to their inaccurate expiration dates. This consumer behavior has a negative financial impact on milk producers. Inaccurate milk spoilage detection methods also force milk producers to use overly conservative expiration dates in an effort to avoid the legal and economic consequences of consumers experiencing illness from drinking spoiled milk. Over the last decade, new methods have been researched with the purpose of developing more accurate and efficient means of detecting milk spoilage. These methods include indicators based on pH bacteria counts and gas-sensor arrays. This article explores various methods of spoilage detection designed to prevent such consequences. The respective level of effectiveness of each method is discussed, as well as several further approaches to contain freshness regardless of detection.
Investigation of Pt/Ta diffusion barrier using hybrid conductive oxide (RuO2) for high dielectric applications (1998)

The Pt/Ta diffusion barrier using hybrid conductive oxide (RuO2) for dynamic random access memory and ferroelectric random access memory capacitor bottom electrodes is proposed. The thermal stability of Pt+RuO2 (50 nm)/Ta+RuO2 (50 nm)/TiSi2/poly-Si/SiO2/Si contact system is investigated and compared to that of the Pt(50 nm)/Ta(50 nm)/TiSi2/poly-Si/SiO2/Si contact system. The Pt+RuO2/Ta+RuO2/TiSi2/poly-Si/SiO2/Si contact system sustained its structure up to 650 °C, whereas Pt/Ta/TiSi2/poly-Si/SiO2/Si contact system was completely degraded after annealing at 650 °C. In the former case, the addition of ruthenium dioxide (RuO2) into the Pt bottom electrode layer led to retardation of the oxygen indiffusion, preventing the indiffusion of oxygen up to 650 °C. In addition, the Ta+RuO2 diffusion barrier showed an amorphous structure and RuO2 is bound to the Ta matrix, inhibiting the interdiffusion of O, Pt, and Si through grain boundaries which can act as fast diffusion paths up to high temperatures. Therefore, it appeared that the barrier properties of Pt/Ta diffusion barrier are improved by using hybrid conductive oxide (RuO2).
Pulmonary hypertension in childhood (1993)

In the presence of pulmonary hypertension, the pulmonary vasculature fails to remodel after birth. Secondary changes then develop, and do so more rapidly than in the adult lung because the cells are exposed to the insult whilst still relatively undifferentiated. The commonest causes of pulmonary hypertension in newborn and young infants are alveolar hypoxia, and congenital heart disease causing a left-to-right shunt and increased pulmonary blood flow. The initial response of an increase in pulmonary arterial muscularity is common to both, but intimal proliferation can develop rapidly in those with complex congenital heart disease. The structural abnormalities are accompanied by abnormalities in the control of pulmonary vascular reactivity, a problem which is the focus of much current research activity.
tiqr: a novel take on two-factor authentication (2011)

Authentication is of paramount importance for all modern networked applications. The username/password paradigm is ubiquitous. This paradigm suffices for many applications that require a relatively low level of assurance about the identity of the end user, but it quickly breaks down when a stronger assertion of the user’s identity is required. Traditionally, this is where two- or multi-factor authentication comes in, providing a higher level of assurance. There is a multitude of two-factor authentication solutions available, but we feel that many solutions do not meet the needs of our community. They are invariably expensive, difficult to roll out in heterogeneous user groups (like student populations), often closed source and closed technology and have usability problems that make them hard to use. In this paper we will give an overview of the two-factor au- thentication landscape and address the issues of closed versus open solutions. We will introduce a novel open standards-based authentication technology that we have developed and released in open source. We will then provide a classification of two-factor authentication technologies, and we will finish with an overview of future work.