gebhart/patrepeval huggingface dataset:
Mechanisms of opioid-induced pain and antinociceptive tolerance: descending facilitation and spinal dynorphin (2001)

Vanderah, Todd W.a,b; Ossipov, Michael H.a,b; Lai, Josephinea,b; Malan, Philip T. Jr.a,b; Porreca, Franka,b,* Author Information
Control of the actinomycin biosynthetic pathway in and actinomycin resistance of Streptomyces spp (1982)

Using actinomycin-producing and nonproducing strains of Streptomyces antibioticus, I studied several steps in the biosynthetic pathway of this antibiotic. Actinomycin-nonproducing strains derived after acriflavine or novobiocin treatment showed activity of kynurenine formamidase and phenoxazinone synthase as high as that of the parental strain, but these nonproducing strains failed to convert 4-methyl-3-hydroxy-anthranilic acid to actinomycin. In addition, accumulation of 4-methyl-3-hydroxyanthranilic acid (in the presence of D-valine) was not detected in the nonproducing isolates. Actinomycin-nonproducing strains derived after acriflavine treatment of Streptomyces parvulus showed a drastic decrease of resistance to the antibiotic. However these strains regained resistance after preincubation with a small amount of actinomycin D.
Estimation of Environmental Lighting from Known Geometries for Mobile Augmented Reality (2013)

Light source estimation and virtual lighting must be believable in terms of appearance and correctness in augmented reality scenes. As a result of illumination complexity in an outdoor scene, realistic lighting for augmented reality is still a challenging problem. In this paper, we propose a framework based on an estimation of environmental lighting from well-defined objects, specifically human faces. The method is tuned for outdoor use, and the algorithm is further enhanced to illuminate virtual objects exposed to direct sunlight. Our model can be integrated into existing mobile augmented reality frameworks to enhance visual perception.
A Generalized Architecture for the Frequency- Selective Digital Predistortion Linearization Technique (2012)

This paper presents a new architecture for the frequency-selective digital predistortion (DPD) for two- and three-band power amplifier (PA) linearization. Also, largely spaced-signal DPD using a digital intermediate frequency (IF) technique is demonstrated. The algorithm used accounts for differential memory effects up to fifth order for bands that can be arbitrarily spaced. The simulation and experimental studies are performed using various signal sets; two- and three-band multitone signals with various tone spacing, band separation, and complementary cumulative distribution function. An improvement of 10 dB over third-order linearization is demonstrated in simulation for more than 20 dB of adjacent channel power ratio reduction. The test signal and the linearization algorithm were implemented on a field-programmable gate array. The linearization algorithm was applied to an RF amplifier at 700-900 MHz. For the two-band case, more than 15 dB on the in-band, 13 dB on the third, and 5 dB on the fifth intermodulation distortion (IMD) cancellation were achieved. For the three-band case, more than 12 dB of IMD cancellation was observed. For largely spaced signal DPD, more than 15 dB of IMD cancellation was achieved. In the three-band case, the linearization of intermodulation byproducts overlapping with the in-band distortion is found to be of critical importance.
B7-H1 is a ubiquitous antiapoptotic receptor on cancer cells (2008)

B7-H1 is an immunoglobulin-like immune suppressive molecule broadly detectable on the majority of human and rodent cancers, and its functions have been attributed to delivering an inhibitory signal to its counter-receptor programmed death-1 (PD-1) on T cells. Here we report that B7-H1 on cancer cells receives a signal from PD-1 to rapidly induce resistance against T cell-mediated killing because crippling signaling capacity of B7-H1 but not PD-1 ablates this resistance. Importantly, loss of B7-H1 signaling is accompanied by increased susceptibility to immune-mediated tumoricidal activity. In addition to resistance against T-cell destruction, B7-H1+ cancer cells also become refractory to apoptosis induced by Fas ligation or the protein kinase inhibitor Staurosporine. Our study reveals a new mechanism by which cancer cells use a receptor on immune cells as a ligand to induce resistance to therapy.
Exploring Electronic versus Steric Effects in Stereoselective Ring‐Opening Polymerization of Lactide and β‐Butyrolactone with Amino‐alkoxy‐bis(phenolate)–Yttrium Complexes (2011)

A series of methoxy-amino-bis(phenol)s (ONOO(R(1),R(2)))H(2) possessing on the phenol rings R(1) ortho substituents with variable steric and electronic properties (R(1)=CMe(2)Ph, 1; CMe(2)tBu, 3; CMe(2)(4-CF(3)C(6)H(4)), 5; CPh(3), 9; Cl, 10) has been synthesized and further reacted with [Y{N(SiHMe(2))(2)}(3)](THF)(2) to give cleanly the corresponding yttrium compounds [Y(ONOO(R(1),R(2))){N(SiHMe(2))(2)}(thf)(n)] (Y-x); the solid-state structures of Y-3 and Y-10 have been determined. These amido complexes have been used as initiators for the ring-opening polymerization (ROP) of rac-lactide (LA) and rac-β-butyrolactone (BBL) to provide heterotactically enriched poly(lactic acid)s (PLAs) and syndiotactically enriched poly(3-hydroxybutyrate)s (PHBs), respectively, by means of a chain-end control mechanism. Most of these polymerizations proceeded in a controlled fashion, giving polymers with narrow polydispersities and experimental molecular weights in good agreement with calculated values. The nature of the R(1) ortho substituents has a profound impact on the rates and, more spectacularly, on the stereocontrol of the polymerizations. The heterotactic stereocontrol in the ROP of rac-LA appears to be governed essentially by steric considerations; the larger the substituent, the higher the heterotacticity: R(1)=Cl (P(r)=0.56)≪CMe(3) (P(r)=0.80)≪CMe(2)Ph (P(r)=0.90)<CMe(2)(4 CF(3)-Ph) (P(r)=0.93-0.94)≤CMe(2)tBu (P(r)=0.94-0.95)≤CPh(3) (P(r)=0.95-0.96). On the other hand, the syndiotactic stereocontrol in the polymerization of rac-BBL follows a quite different trend: R(1)=Cl (P(r)=0.42-0.45)≪CMe(2)tBu (P(r)=0.62-0.70)<CMe(3) (P(r)=0.80)≤CMe(2) (4 CF(3)-Ph) (P(r)=0.82-0.84)<CMe(2)Ph (P(r)=0.89)<CPh(3) (P(r)=0.94), which suggests the involvement of electronic interactions. DFT computations on model intermediates confirmed a stabilizing C-H···π interaction between a methylene C-H of the ring-opened BBL unit and the π system of one of the ortho-aryl substituents of the ONOO(R(1)) ligand; by contrast, for model intermediates in the ROP of LA, no such C-H···π interaction involving the methyl group of lactate was observed.
Additive manufacturing of 3D nano-architected metals (2018)

Most existing methods for additive manufacturing (AM) of metals are inherently limited to ~20-50 μm resolution, which makes them untenable for generating complex 3D-printed metallic structures with smaller features. We developed a lithography-based process to create complex 3D nano-architected metals with ~100 nm resolution. We first synthesize hybrid organic-inorganic materials that contain Ni clusters to produce a metal-rich photoresist, then use two-photon lithography to sculpt 3D polymer scaffolds, and pyrolyze them to volatilize the organics, which produces a >90 wt% Ni-containing architecture. We demonstrate nanolattices with octet geometries, 2 μm unit cells and 300-400-nm diameter beams made of 20-nm grained nanocrystalline, nanoporous Ni. Nanomechanical experiments reveal their specific strength to be 2.1-7.2 MPa g<sup>-1</sup> cm<sup>3</sup>, which is comparable to lattice architectures fabricated using existing metal AM processes. This work demonstrates an efficient pathway to 3D-print micro-architected and nano-architected metals with sub-micron resolution.
Parthenogenetic development of bovine oocytes matured in vitro for 24 hr and activated by ethanol and cycloheximide (1994)

This research was undertaken to improve development of parthenogenetic embryos following various combined treatments of ethanol and cycloheximide. In Experiment 1 in vitro matured oocytes (IVM, 24 hr) were treated with 7% ethanol for 5 min followed by incubation in 10 micrograms/ml cycloheximide in Medium 199 for 0 (control), 5, 10, and 20 hr. Development to 2-8 cells following culture for 3 days was similar among treated groups (32-41%; P > 0.05), which was higher than that of controls (6%; P < 0.05). Experiment 2 compared pre-ethanol exposures for 0, 1, 2.5, and 5 min, followed by 5 hr cycloheximide treatment on activation development. One- to 5-min groups resulted in 42-44% cleavage contrasted to 1-12% for controls (P < 0.05). Experiment 3 examined the effect on oocyte development of ethanol and different concentrations of cycloheximide (0, 1, 5, and 10 micrograms/ml). Cleavage to 2-8 cells was similar among the 5 and 10 micrograms/ml cycloheximide groups (36% and 42%, P > 0.05) but lower (P < 0.05) for the 1 micrograms/ml group (24%) and the controls (2-13%). When 5 micrograms/ml cycloheximide was used (Experiment 4), pre-exposure to ethanol (1, 2.5, and 5 min) resulted in more oocytes cleaved (38-41%) than in the cycloheximide alone group (0%) or the control (0%, P < 0.05). Experiment 5 tested blastocyst development of the activated oocytes with or without cytochalasin B treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
The nucleotide sequence for the cDNA of bovine interleukin-1 alpha (1988)

Journal Article The nucleotide sequence for the cDNA of bovine interleukin-1 alpha Get access Steven R. Leong, Steven R. Leong Department of Developmental Biology, Genentech IncSouth San Francisco, CA 94804, USADepartment of Veterinary Microbiology, University of SaskatchewanSaskatoon, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Gail M. Flaggs, Gail M. Flaggs Department of Developmental Biology, Genentech IncSouth San Francisco, CA 94804, USADepartment of Veterinary Microbiology, University of SaskatchewanSaskatoon, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Michael Lawman, Michael Lawman Department of Developmental Biology, Genentech IncSouth San Francisco, CA 94804, USADepartment of Veterinary Microbiology, University of SaskatchewanSaskatoon, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Patrick W. Gray Patrick W. Gray Department of Developmental Biology, Genentech IncSouth San Francisco, CA 94804, USADepartment of Veterinary Microbiology, University of SaskatchewanSaskatoon, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 16, Issue 18, 26 September 1988, Page 9053, https://doi.org/10.1093/nar/16.18.9053 Published: 26 September 1988 Article history Received: 18 July 1988 Published: 26 September 1988
Excretion and distribution studies in rats with two forms of 14carbon-labelled polyvinylpyrrolidone with a relatively low mean molecular weight after intravenous administration. (1977)

Excretion and autoradiographic distribution studies were performed in rats with two 14C-labelled polyvinylpyrrolidone preparations, both of relatively low mean molecular weight but different in molecular weight distribution. At different time intervals after i.v. administration, elimination of radioactivity was found to be more complete for the preparation with the smallest fraction of molecules having a weight of over 25000, which is approximately the weight limit for rapid glomerular filtration in the rat. Our study demonstrates that the retention of PVP in organs, which in the literature are mentioned as target organs for PVP-toxicity, can be largely prevented by decreasing the fraction of molecules with a weight of more than 25000.
Coding for Flash Memories (2011)

Flash memories are, by far, the most important type of non -volatile memory in use today. They are employed widely in mobile, embedded, and mass-storage applications, and the growth in this sector continues at a staggering pace. Moreover, since flash memories do not suffer from the mechanical limitations of magnetic disk drives, solid- state drives have the potential to upstage the magnetic recording industry in the foreseeable future. The research goal of this dissertation is the discovery of new coding theory methods that supports efficient design of flash memories. Flash memory is comprised of blocks of cells, wherein each cell can take on q ̲&amp;gt; 2 levels. While increasing the cell level is easy, reducing its level can be accomplished only by erasing an entire block. Such block erasures are not only time-consuming, but also degrade the memory lifetime. Our main contribution in this research is the design of rewriting codes that maximize the number of times that information can be written prior to incurring a block erasure. Examples of such coding schemes are flash/floating codes and buffer codes, introduced by Jiang and Bruck et al. in 2007, and WOM- codes that were presented by Rivest and Shamir almost three decades ago. The overall goal in these codes is to maximize the amount of information written to a fixed number of cells in a fixed number of writes. Furthermore, the design of error-correcting codes in flash memories is extensively studied. It is shown how to modify WOM-codes to support an error-correction capability. Motivated by the asymmetry of the error behavior of flash memories and the work by Cassuto et al., a coding scheme to correct asymmetric errors is presented. An extensive empirical database of errors was used to develop a comprehensive understanding of the error behavior as well as to design specific error-correcting codes for flash memories. This research on flash memories is expanded to other directions. Wear leveling techniques are widely used in flash memories in order to reduce and balance block erasures. It is shown that coding schemes to be used in these techniques can significantly reduce the number block erasures incurred during data movement. Also, the design of parallel cell programming algorithms is studied for the specific constraints and behavior of flash cells
G/sub m/-boosted common-gate LNA and differential colpitts VCO/QVCO in 0.18-/spl mu/m CMOS (2005)

The demand for radio frequency (RF) integrated circuits with reduced power consumption is growing owing to the trend toward system-on-a-chip (SoC) implementations in deep-sub-micron CMOS technologies. The concomitant need for high performance imposes additional challenges for circuit designers. In this paper, a g/sub m/-boosted common-gate low-noise amplifier (CGLNA), differential Colpitts voltage-controlled oscillators (VCO), and a quadrature Colpitts voltage-controlled oscillator (QVCO) are presented as alternatives to the conventional common-source LNA and cross-coupled VCO/QVCO topologies. Specifically, a g/sub m/-boosted common-gate LNA loosens the link between noise factor (i.e., noise match) and input matching (i.e., power match ); consequently, both noise factor and bias current are simultaneously reduced. A transformer-coupled CGLNA is described. Suggested by the functional and topological similarities between amplifiers and oscillators, differential Colpitts VCO and QVCO circuits are presented that relax the start-up requirements and improve both close-in and far-out phase noise compared to conventional Colpitts configurations. Experimental results from a 0.18-/spl mu/m CMOS process validate the g/sub m/-boosting design principle.
Printing Proteins as Microarrays for High-Throughput Function Determination (2000)

Systematic efforts are currently under way to construct defined sets of cloned genes for high-throughput expression and purification of recombinant proteins. To facilitate subsequent studies of protein function, we have developed miniaturized assays that accommodate extremely low sample volumes and enable the rapid, simultaneous processing of thousands of proteins. A high-precision robot designed to manufacture complementary DNA microarrays was used to spot proteins onto chemically derivatized glass slides at extremely high spatial densities. The proteins attached covalently to the slide surface yet retained their ability to interact specifically with other proteins, or with small molecules, in solution. Three applications for protein microarrays were demonstrated: screening for protein-protein interactions, identifying the substrates of protein kinases, and identifying the protein targets of small molecules.
Semantic vs. structural resemblance of classes (1991)

We present an approach to determine the similarity of classes which utilized fuzzy and incomplete terminological knowledge together with schema knowledge. We clearly distinguish between semantic similarity determining the degree of resemblance according to real world semantics, and structural correspondence explaining how classes can actually be interrelated. To compute the semantic similarity we introduce the notion of semantic relevance and apply fuzzy set theory to reason about both terminological knowledge and schema knowledge.
IgM antibody-related polyneuropathies: B-cell depletion chemotherapy using Rituximab (1999)

Current treatments for anti-GM1 ganglioside or antimyelin-associated glycoprotein (anti-MAG) antibody-associated polyneuropathies are toxic or very costly. In this preliminary study the authors treated five patients with neuropathy and immunoglobulin M antibodies to GM1 ganglioside or MAG by depleting B cells using Rituximab--a monoclonal antibody directed against the B-cell surface membrane marker CD20. Within 3 to 6 months after treatment, all five patients had improved function, significantly increased quantitative strength measurements, and reduced titers of serum autoantibodies.
Laparoscopic Prosthetic Reinforcement of Hiatal Herniorrhaphy (1999)

The use of PFTE reinforcement for primary repair of large hiatal hernias may result in a lower rate of recurrent herniation compared to primary repair alone.
Predictive robot programming (2003)

One of the main barriers to automating a particular task with a robot is the amount of time needed to program the robot. Decreasing the programming time would facilitate automation in domains previously off limits. In this paper, we present a novel method for leveraging the previous work of a user to decrease future programming time: predictive robot programming. The decrease in programming time is accomplished by predicting waypoints in future robot programs and automatically moving the manipulator end-effector to the predicted position. To this end, we develop algorithms that construct simple continuous-density hidden Markov models by a state-merging algorithm based on waypoints from prior robot programs. We then use these models to predict the waypoints in future robot programs. While the focus of this paper is the application of predictive robot programming, we also give an overview of the underlying algorithms used and present experimental results.
Live births after autologous transplant of cryopreserved mouse ovaries (1997)

The fertility of mice after autologous transplantation of ovaries, before or after cryopreservation, was investigated in this study. Female mice were randomly assigned to either sham-operated (n = 14), ovariectomized (n = 11), fresh (n = 12) or cryopreserved (n = 11) ovarian transplant groups. Ovaries were cryopreserved in 1.4 M dimethyl sulphoxide (DMSO) by cooling to -55 degrees C at 0.5 degree C/min (ice nucleation at -7 degrees C), plunged in liquid nitrogen and then thawed at room temperature. Oestrous cyclicity was observed 7 days after sham operation or 15 days after fresh or cryopreserved ovarian transplant. Ovariectomized animals did not demonstrate oestrous cyclicity but were mated, and no pregnancies resulted. Live births were recorded from all sham-operated, all fresh transplant, and 8/11 (73%) cryopreserved transplant animals. Overall mean +/- SEM litter sizes from fresh (4.32 +/- 0.44) and cryopreserved (4.71 +/- 0.57) transplant groups were smaller (P < 0.05) than those of sham-operated animals (12.54 +/- 0.44), although the sizes were not significantly different (P > 0.05) from each other. Animals were mated at least four times, with four litters of live pups from 4/4 sham-operated, 1/10 fresh and 1/9 cryopreserved ovarian transplant animals. Litter sizes from pups of sham-operated and transplant animals were not significantly different from each other. Following autologous transplantation of mouse ovaries, before or after cryopreservation, offspring appeared normal, with high rates of fertility.
Large bowel fermentation in rats given diets containing raw peas (<i>Pisum sativum</i>) (1990)

The digestion of non-starch polysaccharides (NSP) was studied in rats given semi-purified diets containing 0-500 g raw peas (Pisum sativum)/kg. NSP were equally well digested at all inclusion levels with digestibilities for individual constituents ranging from 0.58 for xylans to 0.99 for arabinose-containing polymers with a total NSP digestibility of 0.79. Increasing the dietary pea inclusion rate increased the amount of substrate flowing to the large bowel (LB) and this was associated with marked increases in caecal tissue and contents masses, a reduction in caecal transit time from 0.88 to 0.43 d and a threefold increase in faecal bacterial output. Caecal pH fell as did the molar proportions of acetate, isobutyrate, isovalerate and valerate whilst butyrate increased when peas were included in the diet. Possible mechanisms for these fermentation end-product changes are discussed. Pea inclusion in the diet was associated with increased volatile fatty acid and 3-hydroxy butyrate concentrations in portal and heart blood. It was concluded that peas are a rich source of fermentable polysaccharides which produce a LB fermentation pattern of potential health benefit.
420-MHz Cr:forsterite femtosecond ring laser and continuum generation in the 1-2-μm range (2003)

We demonstrate a chromium-doped forsterite femtosecond ring laser that generates 30-fs pulses at a 420-MHz repetition rate with nearly 500 mW of average power. The compact solid-state design and broad spectral output make this laser attractive for telecommunications applications in the 1.3-1.5-micrometre region. Additional spectral broadening of the laser output in highly nonlinear optical fiber leads to octave-spanning spectra ranging from 1.06 to 2.17 micrometre. The octave is reached at a level of 18 dB below the peak. The underlying optical frequency comb can be linked to existing optical frequency standards.
MicroRNA-221–222 Regulate the Cell Cycle in Mast Cells (2009)

MicroRNAs (miRNAs) constitute a large family of small noncoding RNAs that have emerged as key posttranscriptional regulators in a wide variety of organisms. Because any one miRNA can potentially regulate expression of a distinct set of genes, differential miRNA expression can shape the repertoire of proteins that are actually expressed during development and differentiation or disease. Here, we have used mast cells as a model to investigate the role of miRNAs in differentiated innate immune cells and found that miR-221-222 are significantly up-regulated upon mast cell activation. Using both bioinformatics and experimental approaches, we identified some signaling pathways, transcription factors, and potential cis-regulatory regions that control miR-221-222 transcription. Overexpression of miR-221-222 in a model mast cell line perturbed cell morphology and cell cycle regulation without altering viability. While in stimulated cells miR-221-222 partially counteracted expression of the cell-cycle inhibitor p27(kip1), we found that in the mouse alternative splicing results in two p27(kip1) mRNA isoforms that differ in their 3' untranslated region, only one of which is subject to miR-221-222 regulation. Additionally, transgenic expression of miR-221-222 from bacterial artificial chromosome clones in embryonic stem cells dramatically reduced cell proliferation and severely impaired their accumulation. Our study provides further insights on miR-221-222 transcriptional regulation as well as evidences that miR-221-222 regulate cell cycle checkpoints in mast cells in response to acute activation stimuli.