gebhart/patrepeval huggingface dataset:
Disposition of liposomal gentamicin following intrabronchial administration in rabbits (1993)

Use of liposomes as carriers of gentamicin for intrabronchial pulmonary delivery was investigated in rabbits. Gentamicin, in isotonic glutamic acid buffer, pH 4.5, was encapsulated in multilamellar vesicles (MLVs) and administered intrabronchially. Higher drug concentrations were found at the pulmonary site of liposome instillation for 1 day as compared with free unencapsulated antibiotic. When time-course distributions of gentamicin given in the liposomal or free form were measured in bronchoalveolar lavages (BAL), similar accumulations were observed up to 4 h, but the drug remained longer (24 h) after administration of the liposomal formulation. Higher amounts of antibiotic were detected in BAL supernatant 1 h after instillation of plain gentamicin; this difference stopped being significant after 4 h. A microbiological assay outlined the bacteriostatic activity of gentamicin released from MLVs and recovered in BAL supernatant. Liposomal gentamicin accumulated in the BAL cell pellet 1 h after intrabronchial instillation; it decreased progressively but minute amounts were still detected after 1 day. On the contrary, no gentamicin was found in the pellet at any time after free drug administration. Comparison of aminoglycoside concentrations in plasma and kidneys indicated lower and constant levels when the liposomal form was instilled. Liposome encapsulation altered the disposition of gentamicin in a way suggesting improved pulmonary concentration and lower systemic toxicity.
TCP Fairness Issues in IEEE 802.11 Networks: Problem Analysis and Solutions Based on Rate Control (2007)

In this paper, we study the problem of maintaining fairness for TCP connections in wireless local area networks (WLANs) based upon the IEEE 802.11 standard. Current implementations of 802.11 use the so-called distributed coordination function (DCF), which provides similar medium access priority to all stations. Although this mode of operation ensures fair access to the medium at the MAC level, it does not provide any provisions for ensuring fairness among the TCP connections. TCP unfairness may result in significant degradation of performance leading to users perceiving unsatisfactory quality of service. We propose and analyze two solutions that are capable of enabling TCP fairness with minimal additional complexity. The proposed solutions are based on utilizing a rate-control mechanism in two modes: static or adaptive. They do not require modifying existing standards at the MAC or network layers. Hence, they are fully compatible with existing devices. Our performance analysis results prove the efficaciousness of our proposed solutions in achieving TCP fairness compared to existing approaches. We have, also, implemented the proposed solutions in an ad-hoc experimental test-bed, and performed measurements to demonstrate the validity of our approach and results
Methyl soyate: A new green alternative solvent (2002)

ADVERTISEMENT RETURN TO ISSUEPREVFeatureNEXTMethyl soyate: A new green alternative solventS. WildesS. WildesMore by S. WildesCite this: Chem. Health Saf. 2002, 9, 3, 24–26Publication Date (Web):May 1, 2002Publication History Published online1 May 2002Published inissue 1 May 2002https://doi.org/10.1016/S1074-9098(02)00292-7Copyright © 2002 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views107Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (90 KB) Get e-Alerts
Hypoxia signaling pathways: modulators of oxygen-related organelles (2015)

Oxygen (O2) is an essential substrate in cellular metabolism, bioenergetics, and signaling and as such linked to the survival and normal function of all metazoans. Low O2 tension (hypoxia) is a fundamental feature of physiological processes as well as pathophysiological conditions such as cancer and ischemic diseases. Central to the molecular mechanisms underlying O2 homeostasis are the hypoxia-inducible factors-1 and -2 alpha (HIF-1α and EPAS1/HIF-2α) that function as master regulators of the adaptive response to hypoxia. HIF-induced genes promote characteristic tumor behaviors, including angiogenesis and metabolic reprogramming. The aim of this review is to critically explore current knowledge of how HIF-α signaling regulates the abundance and function of major O2-consuming organelles. Abundant evidence suggests key roles for HIF-1α in the regulation of mitochondrial homeostasis. An essential adaptation to sustained hypoxia is repression of mitochondrial respiration and induction of glycolysis. HIF-1α activates several genes that trigger mitophagy and represses regulators of mitochondrial biogenesis. Several lines of evidence point to a strong relationship between hypoxia, the accumulation of misfolded proteins in the endoplasmic reticulum, and activation of the unfolded protein response. Surprisingly, although peroxisomes depend highly on molecular O2 for their function, there has been no evidence linking HIF signaling to peroxisomes. We discuss our recent findings that establish HIF-2α as a negative regulator of peroxisome abundance and suggest a mechanism by which cells attune peroxisomal function with O2 availability. HIF-2α activation augments peroxisome turnover by pexophagy and thereby changes lipid composition reminiscent of peroxisomal disorders. We discuss potential mechanisms by which HIF-2α might trigger pexophagy and place special emphasis on the potential pathological implications of HIF-2α-mediated pexophagy for human health.
Functional expression of a plant hydroxynitrile lyase in Escherichia coli by directed evolution: creation and characterization of highly in vivo soluble mutants (2011)

Low protein solubility of recombinantly expressed proteins in Escherichia coli is a major factor hindering their application and analysis. We generated highly in vivo soluble mutants of a hydroxynitrile lyase in E.coli using protein engineering. Structure-guided saturation mutagenesis caused high solubility of single Lys-Pro mutations at positions 176, 199 and 224 of this low soluble wild-type enzyme. The triple Lys-Pro mutant generated at these surface conserved residues showed up to 8-fold increase in specific activity in the cell-free extract. Random mutagenesis also created a mutant of His103Met with 18.5-fold increase. The main expression form was reversed from insoluble to the soluble fraction following both types of above-mentioned mutations in E.coli at 37°C. The findings challenge the rationale of producing recombinant proteins in this host at 37°C. Formerly wild type low soluble protein was then present as soluble protein by these mutations, which also elevated the total soluble protein fraction in E.coli. Saturation mutagenesis of His103 provided other highly soluble mutants with hydrophobic substitutions. These mutations caused only minor secondary structural changes as determined by circular dichroism and Fourier-transform infrared spectroscopy and affected catalytic efficiency slightly for the purified mutants (0.82-1.6-fold for benzaldehyde and 0.9-1.9-fold for mandelonitrile). The stability of the mutants was differed from that of the wild type at high temperatures and at pH >8. Exchanging the buried basic-polar residue His103 with hydrophobic amino acids is in line with the overall structure of the enzyme, i.e. having hydrophilic residues in solvent-exposed areas and hydrophobic residues in the core.
The synergistic activity of αvβ3 integrin and pdgf receptor increases cell migration (1998)

Integrins and growth factor receptors act synergistically to modulate cellular functions. The alphavbeta3 integrin and the platelet-derived growth factor receptor have both been shown to play a positive role in cell migration. We show here that a platelet derived growth factor-BB gradient stimulated migration of rat microvascular endothelial cells on vitronectin (9.2-fold increase compared to resting cells) in a alphavbeta3 and RGD-dependent manner. In contrast, this response was not observed on a beta1 integrin ligand, laminin; background levels of migration, in response to a platelet derived growth factor-BB gradient, were observed on this substrate or on bovine serum albumin (2.4- or 2.0-fold, respectively). Comparable results were obtained using NIH-3T3 cells. Platelet derived growth factor-BB did not change the cells' ability to adhere to vitronectin, nor did it stimulate a further increase in proliferation on vitronectin versus laminin. In addition, platelet derived growth factor-BB stimulation of NIH-3T3 cells did not alter the ability of alphavbeta3 to bind RGD immobilized on Sepharose. The alphavbeta3 integrin and the platelet derived growth factor receptor-beta associate in both microvascular endothelial cells and NIH-3T3 cells, since they coprecipitated using two different antibodies to either alphavbeta3 or to the platelet derived growth factor receptor-beta. In contrast, beta1 integrins did not coprecipitate with the platelet derived growth factor receptor-beta. These results point to a novel pathway, mediated by the synergistic activity of alphavbeta3 and the platelet derived growth factor receptor-beta, that regulates cell migration and, therefore, might play a role during neovessel formation and tissue infiltration.
Competition for the IN service provider (2002)

As global IN standards (CS-1R, CS-2, and AIN 0.2) become more the norm rather than a differentiating factor, it is evident that increasing competition will develop between many different types of service providers, be it a national PTT, multinational corporate conglomerates, local/long distance new entrant, or a private network supplier to large businesses. Competition impacts the way we do business. It can force corporate downsizing, lead to improved quality and new innovative services, and ultimately leads to leaner and meaner structured units. The end result is a better product overall: improved services and reduced rates passed on to the end user. The opening up of a network to a wide variety of service providers creates many challenges as well as opportunities. Some key results of IN competition lie within mediated access and unbundling. Creating an open network with an unbiased access for all service suppliers is the duty of the IN equipment vendor. The creation and marketing of new service concepts is the task of the service provider.
An insight into the role of phosphotransacetylase (pta) and the acetate/acetyl-CoA node in Escherichia coli (2009)

Results showed that pta limits biomass yield in aerobic glucose cultures, due to acetate production (overflow metabolism) and its inefficient use during glucose starvation. Deletion of pta severely impaired growth on acetate minimal medium and under anaerobiosis due to decreased acetyl-coenzyme A synthethase, glyoxylate shunt and gluconeogenic activities, leading to lower growth rate. When acetate is used as carbon source, the joint expression of pta and acs is crucial for growth and substrate assimilation, while pta deletion severely impaired anaerobic growth. Finally, at an adaptive level, pta deficiency makes the strain more sensitive to environmental changes and de-regulates the central metabolism.
Safety and maximum tolerated dose of superselective intraarterial cerebral infusion of bevacizumab after osmotic blood-brain barrier disruption for recurrent malignant glioma (2010)

The authors conclude that SIACI of mannitol followed by bevacizumab (up to 15 mg/kg) for recurrent malignant glioma is safe and well tolerated. Magnetic resonance imaging shows that SIACI treatment with bevacizumab can lead to reduction in tumor area, volume, perfusion, and T2-weighted/FLAIR signal.
Superior cut-off characteristics of L g =40nm W fin =7nm poly Ge junctionless Tri-gate FET for stacked 3D circuits integration (2013)

Poly Ge junctionless (JL) Tri-gate p-FETs with Lg of 40nm and fin width of 7nm has been demonstrated. Major drawbacks in Ge channel such as junction leakage current and DIBL have been overcome by JL FETs with Tri-gate. Ion/Ioff >105 at Vd=-1V, SS=158mV/decade and DIBL of as small as 74mV/V are obtained, which are comparable or better than the counterparts of crystalline Ge devices. Vth roll-off as well as DIBL of JL FETs with thin W are improved in short channel regime.
A 4-channel 10.3Gb/s transceiver with adaptive phase equalizer for 4-to-41dB loss PCB channel (2011)

In this paper, a 4-channel 1.25 to 12.5Gb/s transceiver macro with both phase and amplitude adaptive equalization is presented. It will be shown that when loss at f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">s</sub> /2 exceeds 20dB, phase distortion measurably closes eyes and increases BER. The proposed transceiver cancels 4.1 to 417dB loss for 10.3Gb/s or 4.9 to 34.9dB loss for 12.5Gb/s when both phase and amplitude adaptive equalization are enabled, extending both the length and the range of channels that can be adapted compared to when only amplitude adaptive equalization is used.
Synthesis and antitumor properties of an anthraquinone bisubstituted by the copper chelating peptide Gly-Gly-L-His (1993)

A new molecule 4 [(GGH-DAE)2DHQ] associating the 1,4,5,8-tetrahydroxyanthraquinone ring (DHQ) of the antitumor drug mitoxantrone (2), two diaminoethylene chains (DAE), and the metal-chelating peptide Gly-Gly-His (GGH) has been synthesized. Such a molecule presents characteristics able to induce antitumor activity: compound 4 intercalates into DNA as measured by delta Tm, fluorescence quenching, and viscometry; ESR studies demonstrate that several types of Cu complexes are formed depending on pH; and the production of free radicals, as evidenced by spin-trapping, is enhanced by 4. In vitro, in leukemia cells L1210 and mammary cells MCF7, 4 is slightly less cytostatic than mitoxantrone, but substantially less toxic. In vivo, in leukemia P388 on mice, a T/C value of 230 is obtained at 25 mg/kg, higher than the one of mitoxantrone, which is toxic at the same dose.
Investigation of ultra thin polycrystalline silicon channel for vertical NAND flash (2011)

We have investigated thin film transistors (TFTs) with ultra-thin polycrystalline silicon (poly-Si) of 77 Å – 185 Å. The TFT charge transfer characteristics such as ON current and effective mobility are dominated not by the thickness itself but by the grain size of poly-Si channel. When the poly-Si channel thickness is decreased with the same grain size, the sub-threshold TFT characteristics are improved without degradation of ON current and reliability properties. These results give us appropriate criteria to establish an excellent poly-Si channel in vertical NAND flash memory.
Model-based Information Extraction Method Tolerant of OCR Errors for Document Images (2002)

A new method for information extraction from document images is proposed in this paper as the basis for a document reader which can extract the required keywords and their logical relationship from various printed documents. The proposed method consists of robust keyword matching, global document matching, and postprocessing for matching errors. First, robust keyword matching between two-dimensional OCR results consisting of a set of possible character candidate lists and a set of keywords defined in the keyword dictionary is carried out. This keyword dictionary includes incorrect words with typical OCR errors and segments of words in order to deal with OCR errors. Next, document matching is invoked between keyword matching results in an input document and document models. Each document model consists of a set of word models with their logical relationship described in terms of a tree structure. This model matching extracts the required keywords and their logical relationship from the input document and determines the most suitable model for the input document. Finally, postprocessing using heuristic rules defined in the model is applied to document matching results to recover keyword matching errors and to modify keyword matching results. This comprehensive approach solves word segmentation problems accurately even if a document has unknown words, compound words, or incorrect words due to OCR errors. Experimental results obtained for 100 documents show that the method is robust and effective for various document structures.
An anthropological introduction to YouTube (2008)

Recording of a lecture titled "The Anthropology of YouTube,” given by Michael Wesch on June 23, 2008 at the Library of Congress, Washington, D.C.
Comprehensive Analysis of the 16p11.2 Deletion and Null Cntnap2 Mouse Models of Autism Spectrum Disorder (2015)

Autism spectrum disorder comprises several neurodevelopmental conditions presenting symptoms in social communication and restricted, repetitive behaviors. A major roadblock for drug development for autism is the lack of robust behavioral signatures predictive of clinical efficacy. To address this issue, we further characterized, in a uniform and rigorous way, mouse models of autism that are of interest because of their construct validity and wide availability to the scientific community. We implemented a broad behavioral battery that included but was not restricted to core autism domains, with the goal of identifying robust, reliable phenotypes amenable for further testing. Here we describe comprehensive findings from two known mouse models of autism, obtained at different developmental stages, using a systematic behavioral test battery combining standard tests as well as novel, quantitative, computer-vision based systems. The first mouse model recapitulates a deletion in human chromosome 16p11.2, found in 1% of individuals with autism. The second mouse model harbors homozygous null mutations in Cntnap2, associated with autism and Pitt-Hopkins-like syndrome. Consistent with previous results, 16p11.2 heterozygous null mice, also known as Del(7Slx1b-Sept1)4Aam weighed less than wild type littermates displayed hyperactivity and no social deficits. Cntnap2 homozygous null mice were also hyperactive, froze less during testing, showed a mild gait phenotype and deficits in the three-chamber social preference test, although less robust than previously published. In the open field test with exposure to urine of an estrous female, however, the Cntnap2 null mice showed reduced vocalizations. In addition, Cntnap2 null mice performed slightly better in a cognitive procedural learning test. Although finding and replicating robust behavioral phenotypes in animal models is a challenging task, such functional readouts remain important in the development of therapeutics and we anticipate both our positive and negative findings will be utilized as a resource for the broader scientific community.
ChemInform Abstract: Oxidation of Plasma‐Deposited α‐SixC1‐x:H Films (1990)

Abstract in dry oxygen and air at 500‐900 °C is studied for compositions in the range 0.25 ≦ x ≦ 0.51 and varying hydrogen concentrations.
Experience with Forecasting Univariate Time Series and the Combination of Forecasts (1974)

P. Newbold, C. W. J. Granger, Experience with Forecasting Univariate Time Series and the Combination of Forecasts, Journal of the Royal Statistical Society. Series A (General), Vol. 137, No. 2 (1974), pp. 131-165
Cotransplantation of Allogeneic Islets With Allogeneic Testicular Cell Aggregates Allows Long-Term Graft Survival Without Systemic Immunosuppression (1997)

We prepared single-cell suspensions of Lewis rat ¿RT1(1/l)¿ testicular cells and cultured these in vitro for 48 h under conditions that promoted the formation of cellular aggregates. In the absence of systemic immunosuppression, the transplantation of a sufficient quantity of these aggregates (containing 11 x 10(6) cells, (75% Sertoli cells), together with 2,000 purified Lewis rat islets, reversed the diabetic state for >95 days in 100% (5/5) of the chemically diabetic Wistar-Furth ¿RT1(u/u)¿ recipients. Similar grafts consisting of islets alone or islets plus 50% fewer testicular cell aggregates survived for only 10 days. Functioning composite allografts harvested from normoglycemic animals at approximately 100 days showed healthy beta-cells in close association with Fas ligand-expressing Sertoli cells. Because no gene therapy protocol is required, the transplantation of composite grafts consisting of purified human allogeneic islets plus human allogeneic testicular cell aggregates can be applied in clinical islet transplantation as soon as it has been proven in a large animal model.
Matching with PROSAC — Progressive Sample Consensus (2005)

A new robust matching method is proposed. The progressive sample consensus (PROSAC) algorithm exploits the linear ordering defined on the set of correspondences by a similarity function used in establishing tentative correspondences. Unlike RANSAC, which treats all correspondences equally and draws random samples uniformly from the full set, PROSAC samples are drawn from progressively larger sets of top-ranked correspondences. Under the mild assumption that the similarity measure predicts correctness of a match better than random guessing, we show that PROSAC achieves large computational savings. Experiments demonstrate it is often significantly faster (up to more than hundred times) than RANSAC. For the derived size of the sampled set of correspondences as a function of the number of samples already drawn, PROSAC converges towards RANSAC in the worst case. The power of the method is demonstrated on wide-baseline matching problems.
Reverse transcriptase of human immunodeficiency virus can use either human tRNA(3Lys) or Escherichia coli tRNA(2Gln) as a primer in an in vitro primer-utilization assay. (1992)

Although the reverse transcriptase (RT) of human immunodeficiency virus (HIV) uses human tRNA(3Lys) as a primer of viral genome DNA synthesis in vivo, HIV RT binds Escherichia coli glutamine tRNA and in vitro-made human lysine tRNA with nearly equivalent affinities. We show that HIV RT can use either tRNA(3Lys) or tRNA(2Gln) as a primer for DNA synthesis in vitro without the addition of any other host or viral proteins. E. coli tRNA(2Gln) can serve as a primer for HIV RT if a primer-binding site sequence complementary to the 3' end of tRNA(2Gln) is at the 3' end of the template. With this reduced template, the specificity of binding the proper tRNA is due to base-pairing between a bound tRNA to the primer-binding site of the viral RNA template rather than sequence-specific recognition of tRNA(3Lys) by RT. If an 8-nucleotide viral sequence 3' to the primer-binding site is included in the template, then addition of Zn2+ or Co2+ is required for tRNA(3Lys)-primed synthesis, and tRNA(2Gln) now fails to prime synthesis. The latter result implies that a template sequence adjacent to the primer-binding site and containing 6 nucleotides complementary to the anticodon loop of human tRNA(3Lys) plays an active role in tRNA discrimination.