gebhart/patrepeval huggingface dataset:
Inflammation and the etiology of type 2 diabetes (2005)

Type 2 diabetes is increasingly common worldwide and is beginning to strike younger age groups. Almost 90% of all patients with diabetes show insulin resistance, which also precedes the first symptoms of diabetes. The mechanisms underlying the development of insulin resistance are not well understood. In recent years, several studies have been published that implicate subclinical chronic inflammation as an important pathogenetic factor in the development of insulin resistance and type 2 diabetes. This opens new perspectives for diagnosis and treatment of early insulin resistance and incipient glucose intolerance. Surrogate markers for this low-grade chronic inflammation include CRP, IL-6 and TNF-alpha. Some antidiabetic agents, for example, glitazones that reduce insulin resistance, and insulin itself, reduce inflammation. Conversely, antiinflammatory drugs (ASA/NSAID) may improve glucose tolerance. Vasoactive drugs that are often prescribed to people with diabetes, for example, statins and ACE inhibitors/angiotensin receptor antagonists, also counteract inflammation and reduce the risk of type 2 diabetes. More specific and sensitive biomarkers should be identified, which may predict early disturbances in insulin sensitivity and cardiovascular risk. Also, inflammatory signalling pathways need to be explored in greater detail, and may form the basis of drugable targets against the epidemic of insulin resistance and atherosclerosis.
The Effect of Cuprous Oxide Coatings on Surface Flashover of Dielectric Spacers in Vacuum (1974)

An experimental investigation of the effect of a cuprous oxide coating upon the surface flashover of high-density alumina in vacuum is reported. It is shown that such coatings improve the impulse strength of the system and eliminate the conditioning effect observed in the case of uncoated specimens at dc and 60-Hz voltages. An explanation of the observed behavior is given in terms of a surface charging model. It is postulated that the improvement in the insulation by the coatings is due to a reduction in the secondary electron emission yield.
TCP behavior of a busy Internet server: analysis and improvements (2002)

We analyze the way in which Web browsers use TCP connections based on extensive traffic traces obtained from a busy Web server (the official Web server of the 1996 Atlanta Olympic games). At the time of operation, this Web server was one of the busiest on the Internet. We first describe the techniques used to gather these traces and reconstruct the behavior of the TCP on the server. We then present a detailed analysis of the TCP's loss recovery and congestion control behavior from the recorded transfers. Our two most important results are: (1) short Web transfers lead to poor loss recovery performance for TCPs, and (2) concurrent connections are overly aggressive users of the network. We then discuss techniques designed to solve these problems. To improve the data-driven loss recovery performance of short transfers, we present a new enhancement to the TCP's loss recovery. To improve the congestion control and loss recovery performance of parallel TCP connections, we present a new integrated approach to congestion control and loss recovery that works across the set of concurrent connections. Simulations and trace analysis show that our enhanced loss recovery scheme could have eliminated 25% of all timeout events, and that our integrated approach provides greater fairness and improved startup performance for concurrent connections.
Exploration and Practice of SE-CDIO Educational Pattern (2012)

Taking the requirement and the education goal of software engineering application talented person as guidance, referring to advanced ideas of the international CDIO higher engineering education, combining realities of software engineering undergraduate educates, this paper presents that innovative SE-CDIO pattern for the cultivation of software engineers is developed and implemented in curriculum system, practice teaching and other respects in the software engineering undergraduate specialty of Beijing Information Science & Technology University. Through designing SE-CDIO application personnel cultivation program for software engineering undergraduates, carrying out educational reform practice positively, we raise diligently the quality of software engineering undergraduates who conforms to the discipline and social development need, at the same time promote discipline and specialized development.
One-dimensional hydrogen-bonded molecular tapes in 1,4-bis[(4-pyridinio)ethynyl]benzene chloranilate (2001)

The structure of the title compound, C20H14N22+·C6Cl2O42−, contains one-dimensional hydrogen-bonded molecular tapes along the [113] direction. The molecular tapes are connected via two N—H⋯O hydrogen bonds in R12(5) patterns.
Spinal Sympathetic Neurons: Possible Sites of Opiate-Withdrawal Suppression by Clonidine (1982)

Morphine, methadone, meperidine, fentanyl, and clonidine rapidly depressed transmission through sympathetic preganglionic neurons in cats with the spinal cord transected. Naloxone promptly antagonized this effect of the opiates but not that of clonidine which was reversed by α 2 -adrenergic receptor antagonists. The independent depression of preganglionic neurons by clonidine may contribute to the ability of this drug to depress the symptoms of opiate withdrawal that are characterized by sympathetic hyperactivity.
Matrix/Integrin Interaction Activates the Mitogen-activated Protein Kinase, p44erk-1 and p42erk-2 (1995)

Cell adhesion to extracellular matrix proteins is a dynamic process leading to dramatic changes in the cell phenotype. Integrins are one of the major receptor families that mediate cell-matrix contact. Evidence that integrins can act as signal transducing molecules has accumulated over the past few years. We report here that p44erk-1 and p42erk-2 mitogen-activated protein (MAP) kinases are rapidly phosphorylated on tyrosine residues upon adhesion of human skin fibroblasts to fibronectin or upon cross-linking of beta 1 integrins with antibody. The tyrosine phosphorylation of both kinases is associated with increased enzymatic activity. Pretreatment of the cells with cytochalasin D, which selectively disrupts the network of the actin filaments, completely inhibits this adhesion-mediated MAP kinase activation. Thus, our findings indicate that ligation of beta 1 integrins induces an increase in both tyrosine phosphorylation and enzymatic activity of p44erk-1 and p42erk-2 MAP kinases, and that the integrity of the actin cytoskeleton is essential in this process. Since MAP kinase behaves as a convergence point for diverse receptor-initiated signaling events at the plasma membrane, this serine/threonine kinase plays a key role and helps to account for the diversity of integrin-dependent cell functions.
The complete nucleotide sequence of the <i>lux</i> regulon of <i>Vibrio fischeri</i> and the <i>luxABN</i> region of <i>Photobacterium leiognathi</i> and the mechanism of control of bacterial bioluminescence (1989)

We have determined the complete nucleotide sequence of a 7622 base pair fragment of DNA from Vibrio fischeri strain ATCC7744 that contains all the information required to confer plasmid-borne, regulated bioluminescence upon strains of Escherichia coli. The lux regulon from V. fischeri consists of two divergently transcribed operons, L (left) and R (right), and at least seven genes, luxR (L operon) and luxICDABE (R operon) and the intervening control region. The luxA and luxB genes encode respectively the alpha and beta subunits of luciferase. The gene order luxCDABE seen in V. fischeri is the same as for V. harveyi. We have determined the sequence of the luxAB and flanking regions from Photobacterium leiognathi and have found upstream sequences homologous with luxC from the Vibrio species, but between luxB and luxE, there is an open reading frame encoding a protein of 227 amino acids (26,229 molecular weight) that is not found in this location in the Vibrio species. The amino terminal amino acid sequence of the encoded protein is nearly identical to that determined by O'Kane and Lee (University of Georgia) for the non-fluorescent flavoprotein from a closely related Photobacterium species (Dr Dennis O'Kane, personal communication). We have therefore designated this gene luxN. There is a 20-base inverted repeat ACCTGTAGGAxTCGTACAGGT, centred between bases 927 and 928 in the region between the two operons of V. fischeri. This region appears to fulfil two functions: it is critical for the LuxR protein to exert its effect and it is a consensus binding site for the E. coli LexA protein, a negative regulatory protein involved with the SOS response. There are sequences within the luxR coding region that appear to function in a cis-acting fashion to repress transcription from both the leftward and rightward promoters in the absence of the respective transcriptional activator proteins, thereby resulting in low basal levels of transcription. It now appears clear that there are multiple levels of control on the lux system allowing for a modulation of the intensity of bioluminescence of over four orders of magnitude.
NaN, a novel voltage-gated Na channel, is expressed preferentially in peripheral sensory neurons and down-regulated after axotomy (1998)

Although physiological and pharmacological evidence suggests the presence of multiple tetrodotoxin-resistant (TTX-R) Na channels in neurons of peripheral nervous system ganglia, only one, SNS/PN3, has been identified in these cells to date. We have identified and sequenced a novel Na channel alpha-subunit (NaN), predicted to be TTX-R and voltage-gated, that is expressed preferentially in sensory neurons within dorsal root ganglia (DRG) and trigeminal ganglia. The predicted amino acid sequence of NaN can be aligned with the predicted structure of known Na channel alpha-subunits; all relevant landmark sequences, including positively charged S4 and pore-lining SS1-SS2 segments, and the inactivation tripeptide IFM, are present at predicted positions. However, NaN exhibits only 42-53% similarity to other mammalian Na channels, including SNS/PN3, indicating that it is a novel channel, and suggesting that it may represent a third subfamily of Na channels. NaN transcript levels are reduced significantly 7 days post axotomy in DRG neurons, consistent with previous findings of a reduction in TTX-R Na currents. The preferential expression of NaN in DRG and trigeminal ganglia and the reduction of NaN mRNA levels in DRG after axonal injury suggest that NaN, together with SNS/PN3, may produce TTX-R currents in peripheral sensory neurons and may influence the generation of electrical activity in these cells.
Surface Energy Modified Chips for Detection of Conformational States and Enzymatic Activity in Biomolecules (2006)

A novel patterning method for anchoring biomolecules and noncovalent assembled conjugated polyelectrolyte (CPE)/biomolecule complexes to a chip surface is presented. The surface energy of a hydrophilic substrate is modified using an elastomeric poly(dimethylsiloxane) (PDMS) stamp, containing a relief pattern. Modification takes place on the parts where the PDMS stamp is in conformal contact with the substrate and leaves low molecular weight PDMS residues on the surface resulting in a hydrophobic modification, and then biomolecules and CPE/biomolecule complexes are then adsorbed in a specific pattern. The method constitutes a discrimination system for different conformations in biomolecules using CPEs as reporters and the PDMS modified substrates as the discriminator. Detection of different conformations in two biomacromolecules, a synthetic peptide (JR2E) and a protein (calmodulin), reported by the CPE and resolved by fluorescence was demonstrated. Also, excellent enzyme activity in patterned CPE/horseradish peroxidase (HRP) enzyme was shown, demonstrating that this method can be used to pattern biomolecules with their activity retained. The method presented could be useful in various biochip applications, such as analyzing proteins and peptides in large-scale production, in making metabolic chips, and for making multi-microarrays.
Bone Cancer Pain (2003)

Malignant bone disease creates a unique pain state that involves sensitization of the nervous system. Major contributors to the pain state within the bone tissue are osteoclastic bone resorption and the malignant disease itself.
A new instrument for spatially resolved laser desorption/laser multiphoton ionization mass spectrometry (1993)

A new reflectron time-of-flight mass spectrometer using a pulsed CO2 laser for desorption of neutral molecules, followed by a pulsed, tunable UV laser for resonant multiphoton postionization is described. The innovative features of this instrument are the following: spatially resolved desorption in the μm range, a simple and efficient ion extraction geometry, and several improvements in sample loading, positioning, and observation. Excellent sensitivity in the attomole range and a mass resolution of M/ΔM≊2000 are demonstrated. Primary applications include spatially resolved organic trace analysis and the detection of selected chemical compounds in complex mixtures.
Upscaling Reverse Electrodialysis (2018)

Salinity gradient energy is a sustainable, renewable, and clean energy source. When waters with different salinities are mixed, the change in Gibbs free energy can be harvested as energy and only brackish water remains. Reverse electrodialysis is one of the technologies that can harvest this sustainable energy source. High power densities have been obtained in small lab scale systems, but translation to large industrial scale stacks is essential for commercialization of the technology. Moreover, power density is an important parameter, and efficiency, i.e., the amount of energy harvested compared to the amount of energy available in the feed waters, is critical for commercial processes. In this work, we systematically investigate the influence of stack size and membrane type on power density, thermodynamic efficiency, and energy efficiency. Results show that the residence time is an excellent parameter for comparing differently sized stacks and translating lab scale experimental results to larger pilot stacks. Also, the influence of undesired water permeability and co-ion diffusion (as reflected in permselectivity) is clearly visible when measuring the thermodynamic efficiency. An averaged thermodynamic efficiency of 44.9% is measured using Fujifilm Type 10 anion exchange and cation exchange membranes that have low water permeability and high permselectivity. This value comes close to the thermodynamic maximum of 50%.
Enzyme engineering: immobilized biosystems (1992)

Introduction into enzyme engineering materials for enzyme engineering affinity chromatography immobilized enzymes organelles and cells immobilized biocatalysts in industry and other fields of application.
PHOTOPHYSICAL AND PHOTOSENSITIZING PROPERTIES OF BENZOPORPHYRIN DERIVATIVE MONOACID RING A (BPD‐MA)* (1994)

The photophysical properties of benzoporphyrin derivative monoacid ring A (BPD-MA), a second-generation photosensitizer currently in phase II clinical trials, were investigated in homogeneous solution. Absorption, fluorescence, triplet-state, singlet oxygen (O2 (1 delta g)) sensitization studies and photobleaching experiments are reported. The ground state of this chlorin-type molecule shows a strong absorbance in the red (lambda approximately 688 nm, epsilon approximately 33,000 M-1 cm-1 in organic solvents). For the singlet excited state the following data were determined in methanol: energy level, Es = 42.1 kcal mol-1, lifetime, tau f = 5.2 ns and fluorescence quantum yield, phi f = 0.05 in air-saturated solution. The triplet state of BPD-MA has a lifetime, tau T > or = 25 microseconds, an energy level, ET = 26.9 kcal mol-1 and the molar absorption coefficient is epsilon T = 26,650 M-1 cm-1 at 720 nm. A dramatic effect of oxygen on the fluorescence (phi f) and intersystem crossing (phi T) quantum yields has been observed. The BPD-MA presents rather high triplet (phi T = 0.68 under N2-saturated conditions) and singlet oxygen (phi delta = 0.78) quantum yields. On the other hand, the presence of oxygen does not significantly modify the photobleaching of this photostable compound, the photodegradation quantum yield (phi Pb) of which was found to be on the order of 5 x 10(-5) in organic solvents.
Activity of natural antimicrobial compounds against Escherichia coli and Salmonella enterica serovar Typhimurium (2003)

This study shows the potential of the natural organic compounds to control E. coli and Salm. Typhimurium.
Hybridization chain reaction-based aptameric system for the highly selective and sensitive detection of protein (2012)

We introduce here a novel assay for the detection of platelet-derived growth factor BB (PDGF-BB) via hybridization chain reaction (HCR) based on an aptameric system, where stable DNA monomers assemble only upon exposure to a target PDGF-BB aptamer. In this process, two complementary stable species of biotinylated DNA hairpins coexist in solution until the introduction of initiator aptamer strands triggers a cascade of hybridization events that yields nicked double helices analogous to alternating copolymers. In detail, the aptamer firstly opens the hairpins in the solution, creating long concatemers, and then reacts with the antibody captured PDGF-BB on the well surface. Moreover, several experimental conditions including different PDGF-BB aptamers, the spacer length of the selected aptamer and hairpin, etc. are investigated and optimized. Our results show that the coupling of HCR to aptamer triggers for the amplification detection of PDGF-BB achieves a better performance in the fluorescence detection of PDGF-BB as compared to the traditional antibody-antigen-aptamer assays. Upon modification, the approach presented herein could be extended to detect other types of targets. We believe such advancements will represent a significant step towards improved diagnostics and more personalized medical treatment and environmental monitoring.
The Notch Ligand, Jagged-1, Influences the Development of Primitive Hematopoietic Precursor Cells (1998)

We examined the expression of two members of theNotch family, Notch-1 and Notch-2, and one Notch ligand, Jagged-1, in hematopoietic cells. Both Notch-1 and Notch-2 were detected in murine marrow precursors (Lin−Sca-1+c-kit+). The Notch ligand, Jagged-1, was not detected in whole marrow or in precursors. However, Jagged-1 was seen in cultured primary murine fetal liver stroma, cultured primary murine bone marrow stroma, and in stromal cell lines. These results indicate a potential role for Notch-Notch ligand interactions in hematopoiesis. To further test this possibility, the effect of Jagged-1 on murine marrow precursor cells was assessed by coculturing sorted precursor cells (Lin−Sca-1+c-kit+) with a 3T3 cell layer that expressed human Jagged-1 or by incubating sorted precursors with beads coated with the purified extracellular domain of human Jagged-1 (Jagged-1ext). We found that Jagged-1, presented both on the cell surface and on beads, promoted a twofold to threefold increase in the formation of primitive precursor cell populations. These results suggest a potential use for Notch ligands in expanding precursor cell populations in vitro.
Contingent Genetic Regulatory Events in T Lymphocyte Activation (1989)

Interaction of antigen in the proper histocompatibility context with the T lymphocyte antigen receptor leads to an orderly series of events resulting in morphologic change, proliferation, and the acquisition of immunologic function. In most T lymphocytes two signals are required to initiate this process, one supplied by the antigen receptor and the other by accessory cells or agents that activate protein kinase C. Recently, DNA sequences have been identified that act as response elements for one or the other of the two signals, but do not respond to both signals. The fact that these sequences lie within the control regions of the same genes suggests that signals originating from separate cell membrane receptors are integrated at the level of the responsive gene. The view is put forth that these signals initiate a contingent series of gene activations that bring about proliferation and impart immunologic function.
Quantification of [Dmt <sup>1</sup> ]DALDA in ovine plasma by on‐line liquid chromatography/quadrupole time‐of‐flight mass spectrometry (2003)

The synthetic peptide [Dmt(1)]DALDA (Dmt-D-Arg-Phe-Lys-NH(2); Dmt = 2',6'-dimethyltyrosine; 'super-DALDA') is a mu opioid-receptor agonist. On-line liquid chromatography/quadrupole time-of-flight mass spectrometry and the corresponding stable isotope-incorporated synthetic peptide internal standard were used to quantify [Dmt(1)]DALDA that had been extracted from ovine plasma samples. The [M+2H](2+) ion was used to construct the calibration curve, and the product ion was used for verification of the peptide. The detection sensitivity for the [Dmt(1)]DALDA [M+2H](2+) ion was 12.5 fmol and 50 fmol for the m/z 432.3 product ion. The concentration profile of [Dmt(1)]DALDA was determined from a set of ovine plasma samples. The molecular specificity of the peptide quantification was confirmed by tandem mass spectrometry (MS/MS).
Relative Thermodynamic Stability of DNA, RNA, and DNA:RNA Hybrid Duplexes: Relationship with Base Composition and Structure (1995)

Fourteen oligonucleotides 8-21 nucleotides in length and their complements were synthesized as DNA and RNA. For each sequence, four kinds of duplexes, DNA:DNA, RNA:RNA, DNA:RNA, and RNA:DNA, were prepared. Twelve sequences had A.T/U content varying from 25 to 80% and dPy content in the DNA strands varying from 0 to 100%. Thermodynamic stabilities of four duplexes for each sequence were determined in solution containing 100 mM Na+, 10 mM phosphate, and 0.1 mM EDTA, pH 7.1. CD spectra and electrophoretic mobility on native polyacrylamide gel were measured for most duplexes. Quantitative correlations of hybrid stability both with deoxypyrimidine content and, at fixed dPy content, with the fraction of A.T/U in duplexes were found. We also demonstrated that hybrids with 70-80% deoxypyrimidine DNA strand and a high or moderate A.T/U fraction displayed the highest relative stability compared to their RNA counterparts. Relationships of relative intensities of CD bands at 210 nm and relative electrophoretic mobilities of hybrids with relative hybrid stability suggested that hybrid conformation varies continuously between A- and B-form and is the decisive factor in relative hybrid stability.
Adhesive based flip chip technology for assembly on polyimide flex substrates (2002)

This paper describes research conducted to develop and evaluate isotropically conductive adhesive preforms for the assembly of die onto polyimide flex circuit based MCMs and single chip packages. The preforms are fabricated by screen printing complimentary dielectric and silver filled epoxy patterns corresponding to the die I/O pattern. The dielectric pattern provides a built-in underfill, eliminating the need for a post die placement underfill process. The silver filled epoxy dots provide the electrical connections. The preforms are produced in volume as a multi-up array printed on a release film. In the assembly process, the preform is first aligned and adhered to the die surface. Next the die and preform are aligned to the corresponding flex substrate pattern and bonded. The epoxy is then cured to complete the process. High temperature storage and thermal cycling results are presented.
ChemInform Abstract: DERIVATIVES OF 2‐BENZAZEPINE FROM 3‐SUBSTITUTED 2‐METHYLAMINO‐1,4‐NAPHTHOQUINONES (1976)

Abstract Aus den Alkylaminonaphthochinonen (I) bilden sich unter der Einwirkung von Methylarnin in Gegenwart von Methylamin‐hydrochlorid bei 120‐ 1 70°C (ggf.