gebhart/patrepeval huggingface dataset:
Heat degradation of eukaryotic and bacterial DNA: an experimental model for paleomicrobiology (2012)

In this study, mycobacterial DNA was more resistant to dry-heat stress than eukaryotic DNA. Therefore, the detection of large, experimental, ancient mycobacterial DNA fragments is a suitable approach for paleomicrobiological studies.
Regulatory Mechanisms in the Immune Response to Cell-surface Antigens (1977)

The antigens which matter in immunopathology and cancer are mainly located on the cell surface. To understand the response they provoke, we need to know how material from the cell surface is processed by the immune system. Once the natural mechanisms which regulate this response are understood, we can hope to control and manipulate it. It is to this end that the studies described here are aimed.
Planning and Designing the Data Warehouse (1996)

From the Publisher:
This is a comprehensive survey of key issues associated with planning and designing enterprise data warehouses.Covers the process of implementing a data warehouse end-to-end, from planning a data warehouse, to achieving management support, to implementing metadata repositories that make it easier to access real information, rather than mere data. One chapter is dedicated to helping managers avoid mistakes that can limit the effectiveness of a data warehouse. Once the data warehouse is in place, this book provides guidance on helping end users make the most of it. Two detailed case studies are also included.Information technology managers, and database professionals, including administrators, programmers and designers.
The receptor revolution--multiplicity of G-protein-coupled receptors. (1993)

The superfamily of G-protein-coupled receptors (GPCR) is probably the largest protein-encoding gene family in our genome. It is already known to include hundreds of members and many more are expected to emerge as the molecular cloning revolution proceeds. By definition the GPCR respond to ligands by interacting with intracellular G-proteins and thereby transduce external signals to the interior of the cell. A large body of evidence suggests that the GPCR are organized in the cell membrane like bacteriorhodopsin (BR). All GPCR possess seven hydrophobic membrane-spanning segments which seem to form a characteristic BR-like barrel structure. Thus, the three-dimensional structure of BR may be used as a framework for computer-aided structural modelling of GPCR. The ligands which activate the various members of the GPCR family include an enormous variety of molecules such as amines, amino acids and peptides as well as several small hydrophobic molecules. Many ligands bind to multiple distinct GPCR, e.g. neuropeptide Y (NPY). We have isolated molecular clones encoding a human NPY receptor whose binding properties conform to those of the Y1 subtype. This clone will be a useful tool in our efforts to unravel the molecular mechanisms of the many physiological functions of neuropeptide Y.
Major Complications of Regional Anesthesia in France (2002)

This prospective survey based on a free hotline permanent telephone service allowed us to estimate the incidence of major complications related to regional anesthesia and to provide a detailed analysis of these complications.
An analysis of fast imaging sequences with steady‐state transverse magnetization refocusing (1988)

Recently, several groups have proposed and demonstrated the use of rapid imaging methods, using short pulse repetition times and gradient-reversal echoes. Here, we analyze the behavior of the magnetization and the resulting image contrasts in such sequences for the case where the pulse repetition time TR is of the order of, or shorter than, the transverse relaxation time T2, and the transverse magnetization is not destroyed between phase-encoding cycles. Exact analytical expressions describing the signal evolution between the pulses are derived, taking into account the effects of resonance offsets and flip angles, and examining the influence of constant-phase or alternate-phase RF pulse trains. It is shown that for typical imaging sequences two distinct echo signals will develop between pulses, which may have a detrimental effect on image quality if they partially overlap within the sampling window. It is shown that artifact-free images can be obtained only if the two echo signals overlap precisely, which seems technically close to impossible to achieve, or if they are sufficiently separated in time to allow sampling of only one of the signals.
The immunology of cutaneous DNA immunization. (1999)

The expression of a foreign protein in the skin or muscle following direct in vivo gene transfer results in the induction of potent cellular and humoral immune responses and represents a novel approach for vaccine development. This strategy, now known as genetic or DNA immunization, can be performed either by the bombardment of skin with plasmid DNA coated onto microscopic gold particles using a gene gun, or by injection of muscle with plasmid DNA in saline solution. DNA immunization leads to protective immunity in a variety of rodent models of infectious disease and cancer. Here we will review recent advances in this rapidly expanding research area. Most importantly, the cellular and molecular events leading to the induction of immune responses following DNA inoculation into the skin are now understood in much greater detail. It has become clear that the dendritic cell (DC) system of bone marrow-derived antigen presenting cells (APC) is the initiator of cellular immune responses. Evidence has accumulated that cutaneous DCs are directly transfected leading to endogenous antigen synthesis and processing. The gene transfer procedure results in the activation of DCs and initiates migration to regional lymph nodes, where antigen-expressing DCs efficiently stimulate proliferation of antigen-specific CD8+ as well as CD4+ T-lymphocytes. The nature of the immune response following plasmid DNA immunization may be manipulated by co-delivery of plasmids encoding immunomodulatory cytokines like (interferon) IFNalpha, IL-2 or IL-12 and costimulatory molecules like B7-1. Molecular re-engineering of antigen-encoding plasmids allows for specific targeting of antigen expression into the antigen processing machinery of DC for optimal presentation to CD8+ and CD4+ T-lymphocytes. DNA immunization is currently being developed for the prevention and treatment of a number of infectious diseases and cancer.
Mutational analysis of HPV-18 E6 identifies domains required for p53 degradation in vitro, abolition of p53 transactivation in vivo and immortalisation of primary BMK cells. (1994)

The two major transforming proteins of oncogenic human papillomaviruses are encoded by the E6 and E7 oncogenes. Both viral proteins interact specifically with the products of cellular human tumour suppressor genes; E6 with p53 and E7 with Rb. However, the mechanism of action of E6 is still poorly understood in comparison with that of E7. Although extensive in vitro studies have been done with mutant E6 proteins, very little is known about the activities of E6 in vivo. In this study we have analysed the structure-function relationships of HPV-18 E6 in in vitro analyses and we correlate this with in vivo activity. These studies define a number of domains on the E6 molecule which are involved in the ability of E6 to target p53 for degradation in vitro. This analysis demonstrates that domains previously shown to be important in HPV-16 E6 (Crook et al., 1991; Mietz et al., 1992) are also conserved in HPV-18 and also reconciles the differences between these reports. A series of in vivo studies demonstrate that E6 mediated degradation of p53 in vitro is irrelevant both for cell transformation and for the ability of E6 to abolish p53 transcriptional activation. In addition, we show that at least four distinct regions of the E6 protein are involved in the p53 association in vivo.
Hard rock fragmentation with pulsed power (1993)

Recent work on an electrical method for breaking rock shows that such technology is progressing toward commercial applications. The method, called plasma blasting, is based on the fast delivery of stored electrical energy to fracture the rock. In this paper, an overview of the work done during the last eight years at the Noranda Technology Center is presented. Joint projects between Noranda and Maxwell Laboratories are also introduced, but will be discussecl in more depth in a companion paper. Scaling up of the blast energy from several kilojoules to hundredis of kilojoules is first reviewed. Capacitor storage unit, switching device, cable and blasting electrode used for testing at various (energy levels are described. A discussion of the electrical behavior of the plasma channel created during a blast is then presented, together with computer simulations used for the analysis of test results. An overview of mining equipment based on plasma blasting concludes the discussion.
Benzolamide is not a Membrane-impermeant Carbonic Anhydrase Inhibitor (2004)

Benzolamide, an orphan drug belonging to the pharmacological class of sulfonamide carbonic anhydrase (CA, EC 4.2.1.1) inhibitors (CAIs) is widely used in many physiological and pharmacological studies, together with the clinically employed classical drugs, acetazolamide, methazolamide, ethoxzolamide or dichlorophenamide, it being frequently stated that benzolamide is a membrane-impermeant inhibitor. We prove here that this is false: in fact benzolamide is rather similar to acetazolamide from the point of view of penetrability through blood red cell membranes. Unlike these neutral drugs, the cationic, positively-charged CAIs incorporating either tetraalkyl ammonium or pyridinium moieties, due to their salt-like character are indeed membrane-impermeant, being the only type of low molecular weight compound possessing such properties. Selective inhibition of membrane-associated CA isozymes is relevant indeed in many physiological studies and also pharmacologically, since the tumor-associated isozymes (CA IX and XII) are both membrane-bound.
Resolvin E2 Formation and Impact in Inflammation Resolution (2012)

Acute inflammation and its resolution are essential processes for tissue protection and homeostasis. In this context, specialized proresolving mediators derived from polyunsaturated fatty acids are of interest. In this study, we report that resolvin E2 (RvE2) from eicosapentaenoic acid is endogenously produced during self-limited murine peritonitis in both the initiation and resolution phases. RvE2 (1-10 nM) carries potent leukocyte-directed actions that include: 1) regulating chemotaxis of human neutrophils; and 2) enhancing phagocytosis and anti-inflammatory cytokine production. These actions appear to be mediated by leukocyte G-protein-coupled receptors as preparation of labeled RvE2 gave direct evidence for specific binding of radiolabeled RvE2 to neutrophils (K(d) 24.7 ± 10.1 nM) and resolvin E1 activation of recombinant G-protein-coupled receptors was assessed. In addition to the murine inflammatory milieu, RvE2 was also identified in plasma from healthy human subjects. RvE2 rapidly downregulated surface expression of human leukocyte integrins in whole blood and dampened responses to platelet-activating factor. Together, these results indicate that RvE2 can stimulate host-protective actions throughout initiation and resolution in the innate inflammatory responses.
Antioxidative effect of dietary coenzyme Q10 in human blood plasma. (1994)

The effect of an oral dose of 90 mg/day coenzyme Q10 on the antioxidative status in 22 healthy young subjects (9 men and 13 women) was investigated before and after induction of an oxidative stress by fish oil supplementation. The levels of oxidised and reduced coenzyme Q10, alpha-tocopherol, ascorbate, TBARS and the fatty acid composition of phospholipids were determined in plasma. The total amount of plasma coenzyme Q10 increased significantly from 0.7 +/- 0.1 mumol/l before supplementation to 1.7 +/- 0.3 mumol/l after one week of supplementation while the redox status (reduced CoQ10/total CoQ10) remained constant, even during a following fish oil supplementation. The level of TBARS decreased during the first 2 weeks of CoQ10 ingestion while the content of alpha-tocopherol increased in the second week and ascorbate did not change. The decrease of TBARS and the presence of the majority of the orally supplemented CoQ10 in the reduced form in plasma seem to indicate an antioxidative role of CoQ10 in blood plasma.
Strengthening of liquid crystal photoalignment on azo dye films: passivation by reactive mesogens (2016)

Passivation of photoalignment films by continuous layers of reactive mesogen provides stable liquid crystal alignment and electro-optic performance equivalent to that of rubbed polyimide films.
Fimbria-Mediated Enhanced Attachment of Nontypeable<i>Haemophilus influenzae</i>to Respiratory Syncytial Virus-Infected Respiratory Epithelial Cells (1999)

Respiratory syncytial virus (RSV) infection is known to predispose children to otitis media and sinusitis due to bacteria such as nontypeable Haemophilus influenzae (NTHI). In this study, we investigated the role of NTHI surface outer membrane protein P5-homologous fimbriae (P5-fimbriae) in attachment to RSV-exposed A549 epithelial cells. Analysis by fluorescence flow cytometry showed that a live P5-fimbriated NTHI strain (NTHIF+) attached to a higher proportion of RSV-exposed A549 cells than to control cells (mean, 68% for RSV versus 29% for control; P = 0.008), while attachment of the P5-fimbriae-deficient isogenic mutant strain (NTHIF-) was significantly lower than in control cells and rose only slightly following RSV exposure (mean, 17% for RSV versus 10% for control, P = 0.229). Attachment of NTHIF+ did not correlate with the amount of RSV antigen expressed by A549 cells. Furthermore, paraformaldehyde-fixed NTHIF+ also demonstrated an enhanced binding to RSV-exposed cells. Observations by transmission electronic microscopy showed that the mean number of bacteria attached per 100 RSV-exposed A549 cells was higher for NTHIF+ than NTHIF- (99 versus 18; P < 0.001). No intracellular bacteria were identified. UV-irradiated conditioned supernatants collected from RSV-infected A549 cultures (UV-cRSV) also enhanced the attachment of NTHIF+ to A549, suggesting the presence of a preformed soluble mediator(s) in UV-cRSV that enhances the expression of receptors for P5-fimbriae on A549 cells. In summary, RSV infection significantly enhances NTHI attachment to respiratory epithelial cells. P5-fimbria is the critical appendage of NTHI that participates in this attachment. In clinical settings, blocking of the P5-fimbria-mediated attachment of NTHIF+ by passive or active immunity may reduce the morbidity due to NTHI during RSV infection.
Evaluation of melanogenesis in A-375 melanoma cells treated with 5,7-dimethoxycoumarin and valproic acid (2012)

Malignant melanoma (melanoma malignum) is one of the most dangerous types of tumor. It is very difficult to cure. In recent years, a lot of attention has been given to chemoprevention. This method uses natural and synthetic compounds to interfere with and inhibit the process of carcinogenesis. In this study, a new treatment strategy was proposed consisting of a combination of 5,7-dimethoxycoumarin (DMC), an activator of melanogenesis, and valproic acid (VPA), a well-known drug that is one of the histone deacetylase inhibitors (HDACis). In conjunction with 1 mM VPA, all of the tested concentrations of DMC (10-150 μM) significantly decreased the proliferation of A-375 cells. VPA and DMC also induced the synthesis of melanin and the formation of dendrite and star-shaped cells. Tyrosinase gene expression and tyrosinase activity significantly increased in response to VPA treatment. Pyrolysis with gas chromatography and mass spectrometry (Py-GC/MS) was used to investigate the structure of the isolated melanin. This showed that the quantitative and qualitative components of melanin degradation products are dependent on the type of applied melanogenesis inductor. Products derived from eumelanin were detected in the pyrolytic profile of melanin isolated from A-375 cells stimulated with DMC. Thermal degradation of melanin isolated from melanoma cells after exposure to VPA or a mixture of VPA and DMC revealed the additional presence of products derived from pheomelanin.
Suppression of tumorigenicity of breast cancer cells by transfer of human chromosome 17 does not require transferred BRCA1 and p53 genes. (1995)

A number of candidate tumor suppressor genes located on the human chromosome 17 are thought to have a role to play in the development of breast cancer. In addition to the p53 gene on 17p13.1 and the BRCA1 gene mapped to 17q12-21, other chromosomal regions for tumor suppressor genes have been suggested to exist on 17p13.3 and both the central and the distal parts of 17q, although definitive functional proof of their involvement in breast cancer tumorigenesis is still lacking. In this report we show that microcell transfer of a human chromosome 17 into wild-type p53 breast cancer cells CAL51 results in loss of tumorigenicity and anchorage-independent growth, changes in cell morphology and a reduction of cell growth rates of the neo-selected microcell hybrids. In the hybrid cells, which express the p53 wild-type protein, only the p- and the distal parts of the q arm of donor chromosome 17 are transferred. Thus, our results provide functional evidence for the presence of one or more tumor suppressor gene(s) on chromosome 17, which are distinct from the p53 and the BRCA1 genes.
Spontaneous Ordering of Oxide Nanostructures (2000)

We report the spontaneous formation of uniformly distributed arrays of "tips" (tall conical hillocks) upon oxidation of palladium (Pd) thin films. The formation of the palladium oxide tips depended on the thickness and granularity of the Pd film and on annealing and oxidation conditions. As the Pd film thickness increased from 40 to 200 nanometers, the average height of the tips increased from 0.5 to 1.2 micrometers, their height distribution became broader, and their density decreased from 55 x 10(6) to 12 x 10(6) per square centimeter. Enhanced photoelectron emission from locations corresponding to the tips suggests their possible use in field emission applications.
Correspondence between Smectic Layer Switching and DC Hysteresis of Apparent Tilt Angle in an Antiferroelectric Liquid Crystal Mixture (1990)

We have shown that field-induced antiferro-ferroelectric phase transition is accompanied by reversible smectic layer switching. A layer structure without any electric field applications forms a chevron structure of the same kind as in ordinary SmC * . After the application of a field, the field-induced bookshelf structure relaxes to an obtuse chevron structure. The texture change with focal conics or zigzag defects is interpreted by taking account of the field dependence of the layer tilt angle of the chevron structure. The origin of the layer switching is also discussed on the basis of the texture change.
On decoding of both errors and erasures of a Reed-Solomon code using an inverse-free Berlekamp-Massey algorithm (1999)

In a previous article by Truong et al. (see ibid., vol.46, p.973-76, 1998), it was shown that an inverse-free Berlekamp-Massey (1968, 1969) algorithm can be generalized to find the error locator polynomial in a Reed-Solomon (RS) decoder for correcting errors as well as erasures. The basic idea of this procedure is the replacement of the initial condition of an inverse-free BM algorithm by the Forney (1965) syndromes. It is shown that the errata locator polynomial can be obtained directly by initializing an inverse-free BM algorithm with the erasure locator polynomial and the syndromes. An important ingredient of this new algorithm is a modified BM algorithm for computing the errata locator polynomial. As a consequence, the separate computation of the erasure locator polynomial and the Forney syndrome, needed in the decoder developed by Truong et al., are completely avoided in this modification of the BM algorithm. This modified algorithm requires fewer finite field addition and multiplication operations than the previous algorithm. Finally, the new decoding method was implemented on a computer using C++ language. It is shown in a simulation that the speed of this new decoder is faster than the decoder developed by Truong et al. An example using this program is given for an (255, 239) RS code for correcting errors and erasures with 2/spl nu/+s/spl les/10.
Supramolecular gold nanoparticle–polymer composites formed in water with cucurbit[8]uril (2010)

A gold nanoparticle-polymer composite material has been prepared in water using cucurbit[8]uril as a supramolecular "handcuff" to hold together viologen-functionalised gold nanoparticles and a naphthol-functionalised acrylamide copolymer.
Prevention of impulse noise-induced hearing loss with antioxidants (2004)

Three weeks after exposure, permanent threshold shifts for the experimental groups were significantly reduced to approximately = 10-30 dB less than that for the control group (p < 0.01). Less hair cell loss was also observed in the ALCAR and NAC groups than in the control group.
Surface-plasmon-enhanced fluorescence from periodic quantum dot arrays through distance control using biomolecular linkers (2008)

We have developed a protein-enabled strategy to fabricate quantum dot (QD) nanoarrays where up to a 15-fold increase in surface-plasmon-enhanced fluorescence has been achieved. This approach permits a comprehensive control both laterally (via lithographically defined gold nanoarrays) and vertically (via the QD-metal distance) of the collectively behaving assemblies of QDs and gold nanoarrays by way of biomolecular recognition. Specifically, we demonstrated the spectral tuning of plasmon resonant metal nanoarrays and self-assembly of protein-functionalized QDs in a stepwise fashion with a concomitant incremental increase in separation from the metal surface through biotin-streptavidin spacer units.
Enhanced Stabilization in Dried Silk Fibroin Matrices (2017)

Preliminary studies have shown that silk fibroin can protect biomacromolecules from thermal degradation, but a deeper understanding of underlying mechanisms needed to fully leverage the stabilizing potential of this matrix has not been realized. In this study, we investigate stabilization of plasma C-reactive protein (CRP), a diagnostic indicator of infection or inflammation, to gain insight into stabilizing mechanisms of silk. We observed that the addition of antiplasticizing excipients that suppress β-relaxation amplitudes in silk matrices resulted in enhanced stability of plasma CRP. These observations are consistent with those made in sugar-glass-based protein-stabilizing matrices and suggest fundamental insight into mechanisms as well as practical strategies to employ with silk protein matrices for enhanced stabilization utility.
Ion Solvation by Channel Carbonyls Characterized by<sup>17</sup>O Solid-State NMR at 21 T (2005)

Recently available ultrahigh magnetic fields offer new opportunities for studies of quadrupole nuclei in biological solids because of the dramatic enhancement in sensitivity and resolution associated with the reduction of second-order quadrupole interactions. Here, we present a new approach for understanding the function and energetics of ion solvation in channels using solid-state 17O NMR spectroscopy of single-site 17O-labeled gramicidin A. The chemical shift and quadrupole coupling parameters obtained in powder samples of lyophilized material are similar to those shown in the literature for carbonyl oxygens. In lipid bilayers, it is found that the carbonyl 17O anisotropic chemical shift of Leu10, one of the three carbonyl oxygens contributing to the ion binding site in gramicidin A, is altered by 40 ppm when K+ ion binds to the channel, demonstrating a high sensitivity to such interactions. Moreover, considering the large breadth of the carbonyl 17O chemical shift (>500 ppm), the recording of anisotropic 17O chemical shifts in bilayers aligned with respect to magnetic field B0 offers high-quality structural restraints similar to 15N and 13C anisotropic chemical shifts.