gebhart/patrepeval huggingface dataset:
Screening of Novel Excipients for Improving the Stability of Retroviral and Adenoviral Vectors (2006)

In the past decade there has been an increase in the application of viral vectors in the laboratory and clinical trials of human gene therapy, retroviral and adenoviral vectors among the most used. However, the limited stability of these vectors creates problems in the design of experiments, transport, and storage. Vectors stored at -80 degrees C must be quickly shipped on dry ice, which is somewhat cumbersome. Alternatively, viral vectors can be preserved in a lyophilized form. However, loss of viral activity during lyophilization can also be a serious problem. In this report we identify novel candidate formulations containing new compatible solutes, ectoin, hydroxyectoin, and firoin, that allow better stability of retroviral and adenoviral vectors during storage. For retroviral vectors, the maximum stabilization for long-term storage was achieved through lyophilization followed by storage at -20 degrees C using a formulation of Tris buffer pH 7.2 containing firoin (0.5 M), a half-life of 340 days being obtained. Adenoviral vectors storage at -80 degrees C in solution using Tris buffer pH 8.0 with firoin was the best method for long-term storage, with a half-life exceeding 1 year.
Diverse serologic response to rotavirus infection of infants in a single epidemic (1985)

Eight infants with onset of gastroenteritis occurring within a 32-day period were examined for stool rotavirus antigen and serum antibody response to rotavirus. Each infant presented evidence of rotavirus infection but no single test for rotavirus-specific antigen or antibody was positive for every infected individual. Rotavirus was identified in the stools of five infants; in the others rotavirus etiology was determined by a specific immune response only. The neutralizing antibody response to each of four human rotavirus serotypes and to bovine rotavirus Nebraska calf diarrhea virus ranged from totally negative to a specific response to up to four serotypes. Three originally seropositive infants each showed an increase in neutralizing antibody titer to two or more serotypes. However, two of five originally seronegative infants also developed antibody to two or more serotypes. No consistent pattern of responses to different serotypes was detected.
Methods in Cellular Immunology (2001)

A step-by-step guide to commonly used procedures, Methods in Cellular Immunology addresses both human and murine models, in addition to such topics as PCR and apoptosis. The basic format of the original version has been maintained, and the goal remains the same: to make it a useful and easy-to-use tool for investigators employing cellular immunolog
Biodegradable scleral implant for controlled intraocular delivery of betamethasone phosphate (2000)

We evaluated nail-like, biodegradable scleral implants as a controlled intraocular delivery system of betamethasone phosphate (BP) for the treatment of chronic uveitis using pigmented rabbits. The scleral implants, which contained 10% and 25% of BP (weight 8.5 mg; length 5 mm), were made of poly (D,L-lactide-co-glycolide) (PLGA). In vivo release and retinal toxicity after implantation were also studied in pigmented rabbits. The in vitro release studies demonstrated the 10% and 25% BP-loaded scleral implants released BP in a biphasic release pattern for at least 1 month. The BP concentrations in the vitreous and the retina/choroid after application of scleral implants in pigmented rabbit eyes stayed within the concentration range capable of suppressing inflammatory responses for over 1 month. The BP concentration was greater in the retina/choroid than in the vitreous throughout the study. No substantial toxic reactions in the retina were observed by electroretinography. Our findings suggest that the BP-loaded scleral implant may be a promising device for treatment of chronic uveitis.
Improvement of serum aminotransferase levels after phlebotomy in patients with chronic active hepatitis C and excess hepatic iron. (1994)

Our findings suggest that iron removal may be beneficial for patients with chronic active hepatitis C and histochemical iron in the liver.
Biophysical Characterization and Vector-Specific Antagonist Activity of Domain III of the Tick-Borne Flavivirus Envelope Protein (2001)

The molecular determinants responsible for flavivirus host cell binding and tissue tropism are largely unknown, although domain III of the envelope protein has been implicated in these functions. We examined the solution properties and antagonist activity of Langat virus domain III. Our results suggest that domain III adopts a stably folded structure that can mediate binding of tick-borne flaviviruses but not mosquito-borne flaviviruses to their target cells. Three clusters of phylogenetically conserved residues are identified that may be responsible for the vector-specific antagonist activity of domain III.
Genomic Response to Growth Factors (1988)

Polypeptide growth factors, acting through receptor-mediated pathways, have been shown to induce the sequential expression of specific genes (Cochran et al. 1983; Kelly et al. 1983; Linzer and Nathans 1983; Greenberg and Ziff 1984; Hirschhorn et al. 1984; Lau and Nathans 1985). In quiescent mammalian cells in culture, the first set of genes expressed following addition of growth factor (or serum) are transcriptionally activated even in the presence of an inhibitor of protein synthesis and are therefore thought to be activated directly by second messengers resulting from the growth factor-receptor interaction. These genes have been called "immediate early" genes or "competence" genes. Prior to DNA replication, other genes become active, but, unlike immediate early genes, activation requires new protein synthesis (Katz and Kahana 1987; Linzer and Mordacq 1987), suggesting that one or more proteins encoded by immediate early genes may act as transcriptional regulators in the activation of the "delayed...
Real-time Earthquake Location Using Kirchhoff Reconstruction (2005)

Real-time location of earthquakes can be achieved by using direct imaging of the recorded wave field based on a Kirchhoff reconstruction method similar to that used in the migration of seismic reflection data. The standard method of event location requires the wave arrival at each sensor to be picked and associated with an event. By using direct imaging, the event is identified once in the imaged wave field. The computation is independent of the level of seismic activity and can be carried out on a typical desktop computer. The procedure has been successfully demonstrated in two and three dimensions using data from the Southern California Seismic Network (Trinet). At higher resolutions, the reconstruction method can identify finite source effects. Further work considers extending the method by implementing full elastic theory and solving for moment tensors at all locations in the mesh.
Common animal models for spasticity and pain (2003)

Animal models of spasticity and pain have allowed for the elucidation of possible mechanisms and the evaluation of potential therapeutic interventions for these serious clinical problems. Each model mirrors the clinical appearance of many features of the syndrome, but few reproduce the myriad patient reports of either intensity or relevant contributing factors, especially in models of chronic neuropathic pain. Often these models have been used to predict the potency and efficacy of pharmacologic agents that work in human pain states. Pain models have relied on measurements of the shifts in behavioral hypersensitivity to tactile and thermal stimuli, tests that are not used quantitatively in human patients. Even with the multiple peripheral and central models of spasticity and pain used in animals, only a few actually test human conditions: namely, diabetic neuropathy, chemotherapy, and immunotherapy for tumors. However, all these models have allowed for the comparison of certain behavioral, cellular, biochemical, and molecular mechanisms with human patient populations. Here we review the few extant models of spasticity, nerve injury, and central injury models of pain, and describe their features and use.
Intramuscular partial oxygen tension monitoring in compartment syndrome???an experimental study (1997)

Measuring intracompartmental pressure is a well-accepted method in evaluating a compartment syndrome, which may occur after limb ischaemia followed by reperfusion. As a compartment syndrome is paralleled by a decreased microcirculation it should be possible also to evaluate a compartment syndrome by measuring intramuscular partial oxygen tension (PO2). In this study, anaesthetized rats (spontaneous breathing via tracheotomy) were subjected to infrarenal ligation of the aorta. A pressure catheter was placed subfascial in the crural muscle group of one hind limb, whereas the contralateral side was prepared with a PO2 catheter. Besides a sham operated group, three experimental groups were subjected to either 2, 4 or 6 h of ischaemia followed by 4 h of reperfusion. One further group was also subjected to 4 h of ischaemia and 4 h of reperfusion but received a fasciotomy at the time of reperfusion. Compartment pressure and intramuscular PO2 were recorded every 15 min. For histological examination muscle specimens were obtained after each experiment. Two hours of ischaemia followed by 4 h of reperfusion did not result in any morphological changes and also in no significant change in compartment pressure during both phases, whereas PO2 significantly dropped during ischaemia (from 19.0 mmHg to 3.0-5.0 mmHg) and returned to normal during reperfusion. In prolonged ischaemia (4 h) morphologically a severe interstitial oedema was evident, compartment pressure increased during reperfusion (from 2.0 mmHg to 8.8 mmHg) and PO2 dropped during ischaemia to 3.0 mmHg and did not return to normal during reperfusion (10.5 mmHg versus 19.0 mmHg normal). In the case of 6 h ischaemia, partial necrosis and no interstitial oedema was found morphologically. There was no significant change in compartment pressure throughout the study, and PO2 remained significantly decreased even during reperfusion (2.0-3.0 mmHg). Normal compartment pressure could mislead to false negative interpretation of microcirculatory disorders preceding or following compartment syndrome, whereas PO2 clearly identifies the microcirculatory state of the muscle. Thus, intramuscular PO2 monitoring presents a valuable method in evaluating compartment syndrome, especially where there are suspected clinical signs and risk of ischaemia but normal compartment pressure.
Prediction of Interatrial and Interventricular Electromechanical Delays from P/QRS Measurements: Value for Pacemaker Timing Optimization (2008)

Inter-atrial and inter-ventricular electromechanical delays can be predicted from P-wave and QRS durations. These measurements allow AV delay optimization in DDD and cardiac resynchronization therapy devices with no need of Doppler echocardiography.
The Design of efficient initialization and crash recovery for log-based file systems over flash memory (2006)

While flash memory has been widely adopted for storage systems for various embedded systems, issues of performance and reliability have started receiving growing attention in recent years. How to provide efficient roll back and quick mounting for flash-memory file systems has become an important research topic in recent years, in addition to the work on effective garbage collection and superb runtime performance. Such an observation motivates our work on the investigation of efficient initialization and crash recovery of flash-memory file systems based on log structures. A methodology is proposed for the acceleration of mounting and crash recovery for log-based file systems. A system prototype based on a well-known flash-memory file system, YAFFS, was implemented with performance evaluation. Experimental results show that the proposed methodology can reduce mounting time significantly, regardless of whether the file system is properly unmounted.
Cloning of a functional vesicular GABA and glycine transporter by screening of genome databases (1997)

The unc-47 locus of Caenorhabditis elegans has been suggested to encode a synaptic vesicle GABA transporter. Here we used hydropathy plot analysis to identify a candidate vesicular GABA transporter in genomic sequences derived from a region of the physical map comprising unc-47. A mouse homologue was identified and cloned from EST database information. In situ hybridization in rat brain revealed codistribution with both GABAergic and glycinergic neuronal markers. Moreover, expression in COS-7 and PC12 cells induced an intracellular, glycine-sensitive GABA uptake activity. These observations, consistent with previous data on GABA and glycine uptake by synaptic vesicles, demonstrate that the mouse clone encodes a vesicular inhibitory amino acid transporter.
10 Gbit/s long-wavelength monolithic integratedoptoelectronic receiver grown on GaAs (1996)

The first 10 Gbit/s long-wavelength monolithic integrated photoreceiver grown on GaAs substrate has been fabricated using a 0.3 µm gate length AlGaAs/GaAs HEMT process. At a wavelength of 1.3 µm the integrated InGaAs MSM photodiode has a DC responsivity of 0.34 A/W. The photoreceiver bandwidth is 7.1 GHz and its sensitivity is better than –14.7 dBm (BER = 10-9).
Increased <i>rrn</i> Gene Dosage Causes Intermittent Transcription of rRNA in <i>Escherichia coli</i> (1999)

When the number of rRNA (rrn) operons in an Escherichia coli cells is increased by adding an rrn operon on a multicopy plasmid, the rate of rRNA expression per operon is reduced to maintain a constant concentration of rRNA in the cell. We have used electron microscopy to examine rRNA transcription in cells containing a multicopy plasmid carrying rrnB. We found that there were fewer RNA polymerase molecules transcribing the rrn genes, as predicted from previous gene dosage studies. Furthermore, RNA polymerase molecules were arranged in irregularly spaced groups along the operon. No apparent pause or transcription termination sites that would account for the irregular spacing of the groups of polymerase molecules were observed. We also found that the overall transcription elongation rate was unchanged when the rrn gene dosage was increased. Our data suggest that when rrn gene dosage is increased, initiation events, or promoter-proximal elongation events, are interrupted at irregular time intervals.
Fabrication and evaluation of poly(epsilon‐caprolactone)/silk fibroin blend nanofibrous scaffold (2011)

In this study we investigated the blend electrospinning of poly(ϵ-caprolactone) (PCL) and silk fibroin (SF) to improve the biodegradability and biocompatibility of PCL-based nanofibrous scaffolds. Optimal conditions to fabricate PCL/SF (50/50) blend nanofiber were established for electrospinning using formic acid as a cosolvent and three-dimensional (3D) PCL/SF blend nanofibrous scaffolds were prepared by a modified electrospinning process using methanol coagulation bath. The physical properties of 2D PCL/SF blend nanofiber mats and 3D highly porous blend nanofibrous scaffolds were measured and compared. To evaluate cytocompatibility of the 3D blend scaffolds as compared to 3D PCL nanofibrous scaffold, normal human dermal fibroblasts were cultured. It is concluded that biodegradability and cytocompatibility could be improved for the 3D highly porous PCL/SF (50/50) blend nanofibrous scaffold prepared by blending PCL with SF in electrospinning. In addition to the blending of PCL and SF, the 3D structure and high porosity of electrospun nanofiber assemblies may also be important factors for enhancing the performance of scaffolds.
Inflammatory Cytokine Gene Polymorphisms and Increased Risk of Parkinson Disease (2007)

A smaller magnitude of PD risk increase among carriers of the heterozygous genotype for either or both polymorphisms suggests a gene-dosing effect (OR, 1.45; 95% CI, 0.97-2.16; P<.001 for trend). Results were not sensitive to exclusion of all nonwhite subjects or to adjustment for nonsteroidal anti-inflammatory drug use or smoking.
Coordination chemistry of new selective ethylene trimerisation ligand Ph2PN(iPr)P(Ph)NH(R) (R = iPr, Et) and tests in catalysis (2010)

The synthesis of [Ph(2)PN((i)Pr)P(Ph)NH(R)] (R = (i)Pr, Et) (1, 2) is described and the structure of 2 has been determined by single-crystal X-ray analysis. Compound 1 readily reacts with chromium(0), nickel(0), nickel(II), palladium(II), platinum(II) and iron(II) complexes to give four-membered rings (3-10) via P,P' coordination. The molecular structures of [Cr(CO)(4){Ph(2)PN((i)Pr)P(Ph)NH(R)-P,P'}] (R = (i)Pr, Et) (3, 4), [Cr(CO)(3)(NCCH(3)){Ph(2)PN((i)Pr)P(Ph)NH((i)Pr)-P,P'}] (5), [Ni{Ph(2)PN((i)Pr)P(Ph)NH((i)Pr)-P,P'}(2)] (6), cis-[MX(2){Ph(2)PN((i)Pr)P(Ph)NH((i)Pr)-P,P'}] (M = Ni, Pd, Pt; X = Cl or Br) (7, 8, 9) and trans-[Fe(NCCH(3))(2){Ph(2)PN((i)Pr)P(Ph)NH((i)Pr)-P,P'}(2)](BF(4))(2) (10) have been determined by X-ray diffraction. In the solid state, these complexes show tight phosphine bite angles in the range 67.89(2) degrees to 74.97(4) degrees and the central nitrogen atom adopts an almost planar (sp(2)) geometry. Complexes 3, 5, 6, 7 and 10 are tested for their catalytic activity in ethylene oligomerisation. Additionally, complex 10 is tested in hydrogenation of olefins.
Multialkylation of Aqueous Ammonia with Alcohols Catalyzed by Water-Soluble Cp*Ir−Ammine Complexes (2010)

Novel water-soluble Cp*Ir-ammine complexes have been synthesized, and a new and highly atom-economical system for the synthesis of organic amines using aqueous ammonia as a nitrogen source has been developed. With a water-soluble and air-stable Cp*Ir-ammine catalyst, [Cp*Ir(NH(3))(3)][I](2), a variety of tertiary and secondary amines were synthesized by the multialkylation of aqueous ammonia with theoretical equivalents of primary and secondary alcohols. The catalyst could be recycled by a facile procedure maintaining high activity. A one-flask synthesis of quinolizidine starting with 1,5,9-nonanetriol was also demonstrated. This new catalytic system would provide a practical and environmentally benign methodology for the synthesis of various organic amines.
Homologs of the Yeast Longevity Gene<i>LAG1</i>in<i>Caenorhabditis elegans</i>and Human (1998)

LAG1 is a longevity gene, the first such gene to be identified and cloned from the yeast Saccharomyces cerevisiae. A close homolog of this gene, which we call LAC1, has been found in the yeast genome. We have cloned the human homolog of LAG1 with the ultimate goal of examining its possible function in human aging. In the process, we have also cloned a homolog from the nematode worm Caenorhabditis elegans. Both of these homologs, LAG1Hs and LAG1Ce-1, functionally complemented the lethality of a lag1delta lac1delta double deletion, despite low overall sequence similarity to the yeast proteins. The proteins shared a short sequence, the Lag1 motif, and a similar transmembrane domain profile. Another, more distant human homolog, TRAM, which lacks this motif, did not complement. LAG1Hs also restored the life span of the double deletion, demonstrating that it functions in establishing the longevity phenotype in yeast. LAG1Hs mapped to 19p12, and it was expressed in only three tissues: brain, skeletal muscle, and testis. This gene possesses a trinucleotide (CTG) repeat within exon 1. This and its expression profile raise the possibility that it may be involved in neurodegenerative disease. This possibility suggests at least one way in which LAG1Hs might be involved in human aging.
Performance Evaluation of an SIMD Architecture with a Multi-bank Vector Memory Unit (2006)

The SIMD architecture is very efficient for multimedia data processing since it can handle multiple data with a single instruction. In order to perform an SIMD operation, data must be aligned in the vector register at first, which requires shuffle, pack or unpack instructions and such instructions can be an obstacle to the performance enhancement. The alignment restriction also hinders the efficient automatic vectorization in SIMD compilers. In this paper, an SIMD processor with a multi-bank vector memory unit is designed. The SIMD processor consists of a 2-way VLIW processor, an n-way SIMD co-processor, and an (n+1)-bank vector memory unit. The vector memory unit also includes the address generation logic. An SIMD compiler which exploits the vector memory unit is developed. Since the vector memory permits unaligned and stride accesses without overhead instructions, the developed compiler shows a quite good performance. The performance of an MPEG2 encoder that is optimized by the developed SIMD compiler is analyzed
Electron cooling by arrays of submicron tunnel junctions (1999)

We cooled down the electrons of a 3 μm3 copper strip by using an on-chip electronic microcooler made by a superconductor-insulator-normal-insulator-superconductor (SINIS) structure. We obtained an electron temperature drop ΔT≃−10 mK at a temperature of the bath slightly above Tb=500 mK and we estimated a cooling power slightly larger than 20 pW at this temperature. In contrast to previous experiments, we obtained this result operating with a simple He3 refrigerator. The copper strip temperature change was sensed by measuring the voltage change across a current-biased SINIS junction, with a temperature responsivity ΔV/ΔT≃−500 μV/K. On-chip microcooler and thermometer have been fabricated by two-angle shadow mask evaporation and e-beam lithography.
Immunogold electron microscopic localization of timothy grass (Phleum pratense) pollen major allergens Phl p I and Phl p V after anhydrous fixation in acrolein vapor. (1994)

We used the vapor phase of acrolein as an anhydrous fixative for timothy grass pollen in an immunogold double-labeling localization study of two different major allergens, Phl p I and Phl p V. More than 48 hr of fixation were needed for the subcellular pollen structures to be satisfactorily stabilized. The immunoreactivity of acrolein-fixed pollen allergens was not destroyed even after prolonged acrolein fixation. By immunoblotting, the two allergens differ in their immunological and structural characteristics. Electron microscopic localization traced the allergens at least partially to different subcellular pollen compartments.
Dietary exposure to soy or whey proteins alters colonic global gene expression profiles during rat colon tumorigenesis. (2005)

Neuroblastoma is a pediatric tumor accounting for 15% of childhood cancer deaths and has a poor prognosis in children >1 year of age. We investigated the ability of apigenin, a nonmutagenic dietary flavonoid that has been shown to have antitumor effects in various tumor cell lines, to inhibit growth and induce apoptosis of the human neuroblastoma cell lines NUB-7, LAN-5, and SK-N-BE(2). Apigenin inhibited colony-forming ability and survival, and induced apoptosis of NUB-7 and LAN-5 cells. The presence of the C2-C3 double bond and the 4'-OH group on the flavonoid structure correlated with the growth-inhibitory potential of apigenin. Furthermore, apigenin inhibited NUB-7 xenograft tumor growth in anonobese diabetic/severe combined immunodeficiency mouse model, likely by inducing apoptosis. Apigenin did not inhibit survival of primary sympathetic neurons, suggesting that it is not toxic to nontransformed cells. The mechanism of action of apigenin seems to involve p53, as it increased the levels of p53 and the p53-induced gene products p21WAF1/CIP1 and Bax. Furthermore, apigenin (15-60 micromol/L) induced cell death and apoptosis of neuroblastoma cells expressing wild-type but not mutant p53. Apigenin increased caspase-3 activity and PARP cleavage, and Z-VAD-FMK, a broad-spectrum caspase-3 inhibitor, rescued NUB-7 cells from apigenin-mediated apoptosis indicating that apigenin induced apoptosis in acaspase-dependent manner. Overexpression of Bcl-X(L) rescued NUB-7 from apigenin-induced cell death, suggesting that Bax activity is important for the action of apigenin. Apigenin is thus a candidate therapeutic for neuroblastoma that likely acts by regulating a p53-Bax-caspase-3 apoptotic pathway.