gebhart/patrepeval huggingface dataset:
Multifaceted Intervention by the Hsp90 Inhibitor Ganetespib (STA-9090) in Cancer Cells with Activated JAK/STAT Signaling (2011)

There is accumulating evidence that dysregulated JAK signaling occurs in a wide variety of cancer types. In particular, mutations in JAK2 can result in the constitutive activation of STAT transcription factors and lead to oncogenic growth. JAK kinases are established Hsp90 client proteins and here we show that the novel small molecule Hsp90 inhibitor ganetespib (formerly STA-9090) exhibits potent in vitro and in vivo activity in a range of solid and hematological tumor cells that are dependent on JAK2 activity for growth and survival. Of note, ganetespib treatment results in sustained depletion of JAK2, including the constitutively active JAK2(V617F) mutant, with subsequent loss of STAT activity and reduced STAT-target gene expression. In contrast, treatment with the pan-JAK inhibitor P6 results in only transient effects on these processes. Further differentiating these modes of intervention, RNA and protein expression studies show that ganetespib additionally modulates cell cycle regulatory proteins, while P6 does not. The concomitant impact of ganetespib on both cell growth and cell division signaling translates to potent antitumor efficacy in mouse models of xenografts and disseminated JAK/STAT-driven leukemia. Overall, our findings support Hsp90 inhibition as a novel therapeutic approach for combating diseases dependent on JAK/STAT signaling, with the multimodal action of ganetespib demonstrating advantages over JAK-specific inhibitors.
Biological Control of Plant Pathogens (2006)

Plant diseases need to be controlled to maintain the quality and abundance of food, feed, and fiber produced by growers around the world. Different approaches may be used to prevent, mitigate or control plant diseases. Beyond good agronomic and horticultural practices, growers often rely heavily on chemical fertilizers and pesticides. Such inputs to agriculture have contributed significantly to the spectacular improvements in crop productivity and quality over the past 100 years. However, the environmental pollution caused by excessive use and misuse of agrochemicals, as well as fear-mongering by some opponents of pesticides, has led to considerable changes in people’s attitudes towards the use of pesticides in agriculture. Today, there are strict regulations on chemical pesticide use, and there is political pressure to remove the most hazardous chemicals from the market. Additionally, the spread of plant diseases in natural ecosystems may preclude successful application of chemicals, because of the scale to which such applications might have to be applied. Consequently, some pest management researchers have focused their efforts on developing alternative inputs to synthetic chemicals for controlling pests and diseases. Among these alternatives are those referred to as biological controls.
Optically pumped all-epitaxial wafer-fused1.52 µm vertical-cavity lasers (1994)

The authors demonstrate for the first time the room-temperature pulsed operation of all-epitaxial vertical cavity lasers operating at 1.52 µm using optical pumping. The laser cavity is formed by bonding an MOCVD-grown InGaAsP/InP mirror to an MBE-grown AIAs/GaAs mirror using the wafer fusion technique.
Modulating Pharmacokinetics, Tumor Uptake and Biodistribution by Engineered Nanoparticles (2011)

Neutral and zwitterionic nanoparticles demonstrated longer circulation time via both i.p. and i.v. administration, whereas negatively and positively charged nanoparticles possessed relatively short half-lives. These pharmacological characteristics were reflected on the tumor uptake and biodistribution of the respective nanoparticles, with enhanced tumor uptake by neutral and zwitterionic nanoparticles via passive targeting.
HuM291, A HUMANIZED ANTI-CD3 ANTIBODY, IS IMMUNOSUPPRESSIVE TO T CELLS WHILE EXHIBITING REDUCED MITOGENICITY IN VITRO1 (1999)

When evaluated in vivo, HuM291 may be an immunosuppressive agent associated with less of the acute toxicity and immunogenicity seen with OKT3 therapy.
Drastic reduction of calsequestrin-like proteins and impaired calcium binding in dystrophic<i>mdx</i>muscle (2002)

Although the reduction in dystrophin-associated glycoproteins is the primary pathophysiological consequence of the deficiency in dystrophin, little is known about the secondary abnormalities leading to x-linked muscular dystrophy. As abnormal Ca(2+) handling may be involved in myonecrosis, we investigated the fate of key Ca(2+) regulatory membrane proteins in dystrophic mdx skeletal muscle membranes. Whereas the expression of the ryanodine receptor, the dihydropyridine receptor, the Ca(2+)-ATPase, and calsequestrin was not affected, a drastic decline in calsequestrin-like proteins of 150-220 kDa was observed in dystrophic microsomes using one-dimensional immunoblotting, two-dimensional immunoblotting with isoelectric focusing, diagonal two-dimensional blotting technique, and immunoprecipitation. In analogy, overall Ca(2+) binding was reduced in the sarcoplasmic reticulum of dystrophic muscle. The reduction in Ca(2+) binding proteins might be directly involved in triggering impaired Ca(2+) sequestration within the lumen of the sarcoplasmic reticulum. Thus disturbed sarcolemmal Ca(2+) fluxes seem to influence overall Ca(2+) homeostasis, resulting in distinct changes in the expression profile of a subset of Ca(2+) handling proteins, which might be an important factor in the progressive functional decline of dystrophic muscle fibers.
Use of excipients to control aggregation in peptide and protein formulations (2010)

Aggregation is rapidly emerging as a key issue underlying multiple deleterious effects for peptide or protein-based therapeutics, including loss of efficacy, altered pharmacokinetics, reduced stability or product shelf life, and induction of unwanted immunogenicity. In addition, bioavailability and pharmacokinetics of a self-associating peptide can be influenced by aggregate size and the ease of disruption of the non-covalent intermolecular interactions at the subcutaneous site. This review highlights the various types of aggregates encountered in peptide and protein formulation, methods useful in detecting aggregates, and recent developments in the use of excipients to prevent or reduce peptide and protein aggregation.
Hematopoietic cell transplantation for sickle cell disease: state of the art (2007)

Sickle cell disease is associated with considerable morbidity and premature mortality. Hematopoietic cell transplantation offers the possibility of cure and is associated with excellent results in pediatric patients receiving stem cell transplantation from a matched sibling donor. A reduced-intensity conditioning regimen has the potential to further reduce regimen-related morbidity and mortality. Improved understanding of the natural history of complications, such as stroke and pulmonary hypertension, effects of treatments such as hydroxyurea and blood transfusions, as well as the impact of transplantation on organ damage, are likely to influence the timing and indication of transplantation. Improvements in preparative regimens may enable the safe use of an alternative source of stem cells, such as unrelated matched donors, and further improve the applicability and acceptability of this treatment.
The effects of exogenous nitric oxide on smooth muscle cell proliferation following porcine carotid angioplasty (1995)

These results show for the first time that exogenous nitric oxide inhibits SMC proliferation following balloon angioplasty in vivo. The antiproliferative effects of nitric oxide are overwhelmed when injury is severe and are not associated with a reduction in intimal thickening. The inhibitory effects of nitric oxide on platelet adhesion and SMC proliferation identify a possible role for high local concentrations of nitric oxide to modify the vascular response to balloon angioplasty.
Robotic surgery - the transatlantic case (2003)

This paper presents an historic achievement. A commercial robotic surgical system was altered to enable remote surgery. This presented huge challenges because the Zeus/sup TM/ system is a tightly-coupled electromechanical system with tight sample and communication loops. We were, however, able to separate Zeus/sup TM/ into a remote surgeon-side system (located in New York City) and a patient-side system (located in Strasbourg, France). Never before have surgeons performed complete surgical tasks in the fashion presented in this paper. The remote surgical task was Laparoscopic Cholecystectomy (removal of the gall bladder) performed on animals. In this unique setup the patient (pig) was readied in the operating room in Strasbourg, France, while the operating surgeon was in Manhattan, NY. The Zeus/sup TM/ robotic system is a very safe surgical system for use in the operating room. For the remote transatlantic case, numerous safety features were built on top of those already available in Zeus/sup TM/. The ultimate goal of this development is to perform a transatlantic Lap Chole case on a human subject. As such, the animal cases were performed with the same setup, personnel, and equipment as will be used for the human case. The choices of the robotic system, networking equipment, and telecommunications lines were critical to the success of the project.
Miniaturized Plate Readers for Low-Cost, High-Throughput Phenotypic Screening (2014)

We present a miniaturized plate reader for measuring optical density in 96-well plates. Our standalone reader fits in most incubators, environmental chambers, or biological containment suites, allowing users to leverage their existing laboratory infrastructure. The device contains no moving parts, allowing an entire 96-well plate to be read several times per second. We demonstrate how the fast sampling rate allows our reader to detect small changes in optical density, even when the device is placed in a shaking incubator. A wireless communication module allows remote monitoring of multiple devices in real time. These features allow easy assembly of multiple readers to create a scalable, accurate solution for high-throughput phenotypic screening.
A predistortion linearizer for Ka-band power amplifier in integrated fin-line technique (2011)

This paper presents a millimeter-wave predistortion linearizer, which used a GaAs Schottky diode with a bias feed resistance in integrated fin-line technique. The predistortion line-arizer with a single parallel diode is capable of providing up to 4.5dB gain compensation and 20 degree phase compensation. Combining with the integrated fin-line structures, the linearizer has lower insertion loss, and it is convenient to fabricate especially at millimeter-wave lengths. Experimental results show that the method applied in fin-line is reliable and effective.
Purification of Immunoglobulin G (IgG) (2003)

Several immunological procedures can be successfully carried out using nonpurified antibodies, such as unfractionated antisera, or ascitic fluid/culture supernatant containing monoclonal antibodies (MAbs). However, a much "cleaner" result can often be obtained if some form of enrichment or isolation of immunoglobulin is employed. Some procedures, such as conjugation with isotopes, fluorochromes, or enzymes, and preparation of immunoaffinity columns cannot usually be efficiently performed with nonpurified immunoglobulin, and some procedures may yield artifactual results if whole antiserum or ascitic fluid is used as a source of antibody. Purification of immunoglobulins is therefore essential or, at least, useful for a range of immunological methods. This process may consist of purification of total IgG or subpopulations (e.g., subclasses) of IgG from antisera/ascitic fluid/culture supernatant, or the isolation of a particular antigen binding fraction from such fluids. The former can be achieved by biochemical procedures, whereas the latter usually requires some form of affinity purification.
Synthesis and Surface Acoustic Wave Property of Aluminum Nitride Thin Films Fabricated on Silicon and Diamond Substrates Using the Sputtering Method (2001)

C -axis oriented aluminum nitride (AlN) thin films with a thickness of 1 µm were prepared by reactive DC magnetron sputtering on polycrystalline diamond substrates at a substrate temperature of 623 K. The average surface roughness ( R a ) of the AlN thin films was less than 2 nm obtained by locating the diamond substrates at a position of 100 mm from the aluminum target. The full width at half maximum (FWHM) of the rocking curve for the AlN(002) peak determined by X-ray diffraction analysis was about 0.2°. The surface acoustic wave (SAW) structures were completed by the deposition of aluminum electrodes on the as-deposited AlN surfaces. The SAW characteristics of an interdigital transducer (IDT)/AlN/diamond structure were investigated. The phase velocity and coupling coefficient were 10,120 m/s and 0.3%, respectively.
The influence of oxygen tension on the long‐term growth <i>in vitro</i> of haematopoietic progenitor cells from human cord blood (1986)

Growth of low density human cord blood cells in long-term suspension culture was evaluated at incubation conditions of 5% carbon dioxide in approximately 20% (normal incubator) oxygen tension or in 5% (low) oxygen tension. During the first 5 weeks there was no difference in the numbers of morphologically recognizable cells grown at either oxygen tension, while growth was superior from weeks 5 to 8 at approximately 20% oxygen tension. For the first 5 weeks, growth of granulocyte-macrophage progenitors (CFU-GM) was superior at 5% oxygen tension during the long-term and semi-solid culture phases and least impressive in the long-term and semi-solid cultures incubated at approximately 20% oxygen. This trend was reversed after 5 weeks and after 8 weeks there were no detectable CFU-GM in suspension cultures at 5% oxygen while steady state levels of CFU-GM were maintained for greater than 12 weeks in suspension cultures at approximately 20% oxygen. Semi-solid cultures for erythroid (BFU-E) and multipotential (CFU-GEMM) progenitors were incubated at approximately 20% oxygen only. During the first 4 weeks, the growth of BFU-E and CFU-GEMM was superior at 5% oxygen during long-term culture. Numbers of these cells decreased by week 5 and at this time growth was better in the long-term cultures grown at approximately 20% oxygen. By week 6, no BFU-E or CFU-GEMM were detectable. Thus, growth of cord blood at low oxygen tension gives an initial enhancement in output of progenitor cells, but this appears to be at the expense of continued production.
Polarographic Measurement of Steady State Kinetics of Oxygen Uptake by Biochemical Samples (1980)

This chapter contains sections titled: Introduction Theory of Measurement Instrumentation Applications
Extraction of carboxylic acids with amine extractants. 1. Equilibria and law of mass action modeling (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTExtraction of carboxylic acids with amine extractants. 1. Equilibria and law of mass action modelingJanet A. Tamada, A. Steven Kertes, and C. Judson KingCite this: Ind. Eng. Chem. Res. 1990, 29, 7, 1319–1326Publication Date (Print):July 1, 1990Publication History Published online1 May 2002Published inissue 1 July 1990https://pubs.acs.org/doi/10.1021/ie00103a035https://doi.org/10.1021/ie00103a035research-articleACS PublicationsRequest reuse permissionsArticle Views2187Altmetric-Citations362LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Automatic acquisition of layout knowledge for understanding business cards (2002)

Document knowledge plays a very important role in many document image understanding methods. In these methods, the document knowledge is utilized to classify/extract individual item data interpretatively from paper-based sheets as a kind of document model: these days, this knowledge is specified into the document image understanding system in advance. In this paper, we propose an experimental method to acquire the layout knowledge automatically from sample document images. In particular, we focus on the acquisition of the layout of business cards. Our idea is to generate the layout knowledge of business cards from a predefined logical structure, which is used as a kind of meta-knowledge to interpretatively generate the layout knowledge of given business cards.
Amino acid and cDNA sequences of a methionine‐rich 2S protein from sunflower seed (<i>Helianthus annuus</i> L.) (1991)

The amino acid sequence of a methionine‐rich 2S seed protein from sunflower ( Helianthus annuus L.) and the sequence of a cDNA clone which codes for the entire primary translation product have been determined. The mature protein consists of a single polypeptide chain of 103 amino acids (molecular mass 12 133 Da) which contains 16 residues of methionine and 8 residues of cysteine. The cDNA sequence established that the protein is synthesized as a precursor of 141 residues with a typical hydrophobic signal sequence of 25 residues followed by a further 13‐residue hydrophobic pro‐sequence which is presumably removed by post‐translational cleavage. The sequence of the mature protein and that deduced from the cDNA were identical with no evidence of processing at the C‐terminus. Comparison of the sunflower methionine‐rich protein sequence with sequences of other seed 2S proteins from dicotyledons and monocotyledons showed limited but distinct sequence similarities; in particular the arrangement of the cysteine residues was conserved. The sunflower protein shows 34% identity with the methionine‐rich Brazil nut 2S protein and the prepro regions of the precursors of these two proteins show about 50% identity. This similarity indicates that these methionine‐rich 2S proteins have diverged as a subclass of the 2S superfamily of proteins which contain only 2–3% methionine. While the related 2S proteins from other dicotyledons are processed to a small and large subunit, the sunflower protein is not cleaved in this way.
A task allocation scheme for hypercube distributed computing systems using the affinity graph model (2003)

In the affinity graph model, the vertices represent the modules in the task to be allocated. The weight of the edges represents the affinity the modules represented by the vertices have for each other. The affinity function has been defined in such a way that both the competing demands of load balancing and minimizing interprocessor communication are addressed. By applying a graph partitioning algorithm on such an affinity graph, optimal task allocation is possible. The task allocation algorithm presented for hypercube distributed computing systems uses the above idea to repeatedly partition the affinity graph until all modules in the task are allocated. The algorithm is fully distributed with no central control being exercised.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Chemotherapy for major food-borne trematodes: a review (2004)

Food-borne trematode infections, caused by liver flukes (Clonorchis, Fasciola, Opisthorchis), lung flukes (Paragonimus) and intestinal flukes (Echinostoma, Fasciolopsis, heterophyids), are significant public health problems, most notably in Southeast Asia and the Western Pacific. Globally, it is estimated that > 40 million people are infected among the 750 million people who live in endemic areas. The epidemiology of food-borne trematodiasis has changed over the past few decades, and now presents a dual picture. On the one hand, increasing numbers of infections are reported from non-endemic areas, and endemic areas are expanding due to larger areas utilised for aquaculture, domestic migration, declining socioeconomic conditions, lack of improved sanitation, and increasing availability of aquatic foods through wider distribution networks often without proper food inspections. On the other hand, social and economic advances in many Asian countries, going hand-in-hand with urbanisation, use of chemical fertilisers and, above all, the administration of safe, efficacious and inexpensive drugs, have significantly reduced the prevalence of food-borne trematode infections. In this review, the taxonomy, life cycle, and geographical distribution of the major food-borne trematodes, including issues of diagnosis and clinical disease manifestations, is summarised. The discovery, chemistry, pharmacological properties, safety, therapeutic efficacy and adverse effects of the current drugs of choice, namely praziquantel and triclabendazole, is then discussed. Recent advances on other drugs and contemporary investigations on novel compounds that might become important players in chemotherapy are highlighted. Finally, the need for research and development of new trematocidal drugs that - employed in concert with health education, improved sanitation and enhanced food safety - are key factors for sustainable control of food-borne trematodiasis, is highlighted.
Isolated thrombosis due to the cystathionine β‐synthase mutation c.833T&gt;C (I278T) (2003)

Hereditary homocystinuria due to cystathionine beta-synthase (CBS) deficiency is a rare disease (about 1:20000 in Germany) often complicated by thromboembolism. Single mutations, which affect the C-terminal region of the CBS enzyme, lead to isolated thrombosis without further symptoms typical for homocystinuria such as atherosclerosis, psychomotor retardation, and dislocation of the ocular lenses. In this study, DNA samples of patients with stroke (n = 225) and sinus thrombosis (n = 46) were screened for the most common homocystinuria mutation, CBS 1278T. In each group one homozygous patient was identified. Thus, not only C-terminal mutations but also the most common mutation in classical homocystinuria, CBS 1278T, can lead to isolated thrombophilic events. These data support the hypothesis that homocystinuria is an underdiagnosed disease.
A Decreased Level of Serum Soluble Klotho Is an Independent Biomarker Associated with Arterial Stiffness in Patients with Chronic Kidney Disease (2013)

Decreases in the serum soluble Klotho levels are independently associated with signs of vascular dysfunction such as arterial stiffness in patients with CKD. Further research exploring whether therapeutic approaches to maintain or elevate the Klotho level could improve arterial stiffness in CKD patients is warranted.
Improvement of Collaborative Filtering Based on Fuzzy Reasoning Model (2006)

Though various contents are provided through the internet recently, it is not easy to collect favorite contents among huge amounts of contents in terms of user's preference. In this paper, we focus on the collaborative filtering algorithm in the recommender system. We propose a fuzzy modeling approach for preference similarity model in collaborative filtering. In our approach, valid simplified fuzzy model is constructed through optimization of MAE(Mean Absolute Error). The model decides the weights of preference similarity from the value of correlation coefficient and the number of items. Through numerical experiments compared with conventional correlation coefficient based approach using Movie Lens data, the approach is found to be promising for improvement of collaborative filtering model accuracy.