gebhart/patrepeval huggingface dataset:
Enhancement of Critical Heat Flux From High Power Microelectronic Heat Sources in a Flow Channel (1990)

Several surface augmentation techniques were examined in an investigation of enhancement of critical heat flux (CHF) from a simulated electronic chip to a fluorocarbon (FC-72) liquid in a vertical channel. A parametric comparison of boiling performances is presented for a smooth surface and for surfaces with low-profile microgrooves, low-profile microstuds, and high-profile pin fins. Critical heat fluxes as high as 361 W/cm2 were achieved using a combination of moderate flow velocity, high subcooling and surface enhancement. A semiempirical model constructed previously for CHF from a smooth discrete heat source to saturated or subcooled liquid flow, was found successful in correlating CHF data for the three enhanced surfaces.
Synthesis of main group heterocycles by metallacycle transfer from zirconium (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of main group heterocycles by metallacycle transfer from zirconiumPaul J. Fagan and William A. NugentCite this: J. Am. Chem. Soc. 1988, 110, 7, 2310–2312Publication Date (Print):March 1, 1988Publication History Published online1 May 2002Published inissue 1 March 1988https://pubs.acs.org/doi/10.1021/ja00215a057https://doi.org/10.1021/ja00215a057research-articleACS PublicationsRequest reuse permissionsArticle Views2347Altmetric-Citations310LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Denaturing high performance liquid chromatography (DHPLC) used in the detection of germline and somatic mutations (1998)

Denaturing high performance liquid chromatography (DHPLC) has been described recently as a method for screening DNA samples for single nucleotide polymorphisms and inherited mutations. Thirty-eight DNAs, 22 of which were heterozygous for previously characterized rearranged transforming gene (RET) or cystic fibrosis transmembrane conductance regulator gene (CFTR) mutations or polymorphisms, were examined using DHPLC analysis to assess the accuracy of this scanning method. Ninety-one per cent (20/22) of the PCR amplicons from specimens with heterozygous RET or CFTR sequence showed elution profiles distinct from corresponding homozygous normal patterns; whether the profiles for two amplicons containing heterozygous RET sequence were distinct from homozygous cases was equivocal. To investigate the usefulness of this method for detecting mutations in tumor DNAs, each of the phosphatase and tensin homologue deleted on chromosome ten gene (PTEN) exons were examined for mutations in 63 malignant gliomas. Seventeen PTEN PCR products from this series of brain tumors showed elution profiles indicating sample heterozygosity and in each instance conventional sequencing confirmed the presence of a mutation. PTEN amplicons containing exons 1, 3 and 5 were sequenced for each of the 63 tumor DNAs to determine whether any mutations may have escaped DHPLC detection, and this analysis identified one such alteration in addition to the eight mutations that DHPLC had revealed. In total, DHPLC identified 37 of 40 (92.5%) PCR products containing defined sequence variation and no alterations were indicated among 196 amplicons containing homozygous normal sequence.
Quantification of Melanoma Cell-specific MART-1 mRNA in Peripheral Blood by a Calibrated Competitive Reverse Transcription-PCR (2000)

Our method is the first competitive RT-PCR assay for quantification of melanoma cells in blood samples that compensates for the variation of both the reverse transcription and PCR reactions. The method allows the inclusion of control samples for continuous quality assessment.
Elimination of unaltered DNA in mixed clinical samples via nuclease-assisted minor-allele enrichment (2016)

Presence of excess unaltered, wild-type (WT) DNA providing no information of biological or clinical value often masks rare alterations containing diagnostic or therapeutic clues in cancer, prenatal diagnosis, infectious diseases or organ transplantation. With the surge of high-throughput technologies there is a growing demand for removing unaltered DNA over large pools-of-sequences. Here we present nuclease-assisted minor-allele enrichment with probe-overlap (NaME-PrO), a single-step approach with broad genome coverage that can remove WT-DNA from numerous sequences simultaneously, prior to genomic analysis. NaME-PrO employs a double-strand-DNA-specific nuclease and overlapping oligonucleotide-probes interrogating WT-DNA targets and guiding nuclease digestion to these sites. Mutation-containing DNA creates probe-DNA mismatches that inhibit digestion, thus subsequent DNA-amplification magnifies DNA-alterations at all selected targets. We demonstrate several-hundred-fold mutation enrichment in diverse human samples on multiple clinically relevant targets including tumor samples and circulating DNA in 50-plex reactions. Enrichment enables routine mutation detection at 0.01% abundance while by adjusting conditions it is possible to sequence mutations down to 0.00003% abundance, or to scan tumor-suppressor genes for rare mutations. NaME-PrO introduces a simple and highly parallel process to remove un-informative DNA sequences and unmask clinically and biologically useful alterations.
Bright functional rotaxanes (2009)

As a hot topic in research, various rotaxanes continue to be constructed. This tutorial review focuses mainly on bright rotaxanes with functional properties, which have been developed in recent years: fluorescent rotaxanes, rotaxanes as logic gates and information storage devices, gelation of rotaxanes, rotaxanes on solid surfaces, sensory rotaxanes, as well as rotaxanes related to biology and so on. Novel synthetic protocols towards several functional rotaxanes are also illustrated.
Menu-based natural language understanding (1984)

Menu-Based Natural Language Understanding is a new approach to building natural language interfaces. It retains the main goals of natural language systems: flexibility, expressive power, learnability and mnemonicity. However, it solves most of the problems inherent to conventional natural language systems. All queries are understood by the system, interface generation is much simpler, and less computing power is required. Many interfaces have been built using the menu-based natural language technology.
An agent-based flexible routing manufacturing control simulation system (1994)

We present a manufacturing simulation system based on autonomous agents and a dynamic price mechanism that explores routing flexibility and provides a programming language for modeling manufacturing environments, based on autonomous agents and a dynamic price mechanism. The simulation system contains a control simulation module and a manufacturing environment simulation module. The control simulation module consists of a collection of autonomous agents who negotiate with each other to reach job processing decisions based on negotiation protocols and built-in price adjustment algorithms. The manufacturing environment simulation module is an event-based simulation system that couples with an input simulation language called Flexible Routing Adaptive Control Simulation Language. The language can be used to model complicated manufacturing environments and to specify flexibility in part process plans. By integrating the control framework with the FRACS simulation system, a sophisticated test bed is created for research of different control and negotiation strategies. The simulation system allows quick turn around time for software prototyping, and the modeling language enables easy model adjustment and performance tuning. Many experiments in the control and scheduling of manufacturing systems have been conducted using this simulation system. Several of these experiments are discussed in the paper.
Mutation Analysis of the Entire Replicated Portion of PKD1 Using Genomic DNA Samples (2001)

The replicated portion of PKD1, which comprises nearly 70% of the length of the gene, is predicted to harbor at least 85% of the mutations present in affected autosomal dominant polycystic kidney disease type 1 pedigrees. The relative paucity of reported mutations involving this segment is attributable to the significant technical challenges posed by the genomic structure of the gene. Previous genomic DNA-based strategies were unable to evaluate exons 1 and 22 and relied on the use of 10- to 13-kb PCR products. In this report, a set of six novel primer pair combinations, which can be used with previously reported reagents to analyze all of the exons in the replicated region (exons 1 to 34), are described. No product is greater than 5.8 kb in length, and various primer combinations can be used to reduce this length in half. Using this approach, two new pathogenic mutations, four novel disease-associated missense substitutions, and six new normal variants were identified. These new reagents should prove useful to investigators interested in performing DNA testing for this disorder.
The CbrA–CbrB two‐component regulatory system controls the utilization of multiple carbon and nitrogen sources in <i>Pseudomonas aeruginosa</i> (2001)

A novel two-component system, CbrA-CbrB, was discovered in Pseudomonas aeruginosa; cbrA and cbrB mutants of strain PAO were found to be unable to use several amino acids (such as arginine, histidine and proline), polyamines and agmatine as sole carbon and nitrogen sources. These mutants were also unable to use, or used poorly, many other carbon sources, including mannitol, glucose, pyruvate and citrate. A 7 kb EcoRI fragment carrying the cbrA and cbrB genes was cloned and sequenced. The cbrA and cbrB genes encode a sensor/histidine kinase (Mr 108 379, 983 residues) and a cognate response regulator (Mr 52 254, 478 residues) respectively. The amino-terminal half (490 residues) of CbrA appears to be a sensor membrane domain, as predicted by 12 possible transmembrane helices, whereas the carboxy-terminal part shares homology with the histidine kinases of the NtrB family. The CbrB response regulator shows similarity to the NtrC family members. Complementation and primer extension experiments indicated that cbrA and cbrB are transcribed from separate promoters. In cbrA or cbrB mutants, as well as in the allelic argR9901 and argR9902 mutants, the aot-argR operon was not induced by arginine, indicating an essential role for this two-component system in the expression of the ArgR-dependent catabolic pathways, including the aruCFGDB operon specifying the major aerobic arginine catabolic pathway. The histidine catabolic enzyme histidase was not expressed in cbrAB mutants, even in the presence of histidine. In contrast, proline dehydrogenase, responsible for proline utilization (Pru), was expressed in a cbrB mutant at a level comparable with that of the wild-type strain. When succinate or other C4-dicarboxylates were added to proline medium at 1 mM, the cbrB mutant was restored to a Pru+ phenotype. Such a succinate-dependent Pru+ property was almost abolished by 20 mM ammonia. In conclusion, the CbrA-CbrB system controls the expression of several catabolic pathways and, perhaps together with the NtrB-NtrC system, appears to ensure the intracellular carbon: nitrogen balance in P. aeruginosa.
Human-Computer Music Performance: From Synchronized Accompaniment to Musical Partner (2013)

Live music performance with computers has motivated many research projects in science, engineering, and the arts. In spite of decades of work, it is surprising that there is not more technology for, and a better understanding of the computer as music performer. We review the development of techniques for live music performance and outline our efforts to establish a new direction, Human-Computer Music Performance (HCMP), as a framework for a variety of coordinated studies. Our work in this area spans performance analysis, synchronization techniques, and interactive performance systems. Our goal is to enable musicians to ncorporate computers into performances easily and effectively through a better understanding of requirements, new techniques, and practical, performance-worthy implementations. We conclude with directions for future work.
Blockchain-Based Supply Chain Traceability: Token Recipes Model Manufacturing Processes (2018)

Growing consumer awareness as well as manufacturers' internal quality requirements lead to novel demands on supply chain traceability. Existing centralized solutions suffer from isolated data storage and lacking trust when multiple parties are involved. Decentralized blockchain-based approaches attempt to overcome these shortcomings by creating digital representations of physical goods to facilitate tracking across multiple entities. However, they currently do not capture the transformation of goods in manufacturing processes. Therefore, the relation between ingredients and product is lost, limiting the ability to trace a product's provenance. We propose a blockchain-based supply chain traceability system using smart contracts. In such contracts, manufacturers define the composition of products in the form of recipes. Each ingredient of the recipe is a nonfungible token that corresponds to a batch of physical goods. When the recipe is applied, its ingredients are consumed and a new token is produced. This mechanism preserves the traceability of product transformations. The system is implemented for the Ethereum Virtual Machine and is applicable to any blockchain configuration that supports it. Our evaluation reveals that the gas costs scale linearly with the number of products considered in the system. This leads to the conclusion that the solution can handle complex use cases.
Drug Compatibility with a New Generation of VISIV Polyolefin Infusion Solution Containers. (2015)

A new generation of VISIV polyolefin intravenous solution containers, made of a new and different proprietary polymer, were evaluated for sorption and leaching potential with a cadre of drugs known to exhibit those phenomena with polyvinylchloride containers. Sorption potential was evaluated for amiodarone hydrochloride, carmustine, regular human insulin, lorazepam, nitroglycerin, sufentanil citrate, and thiopental sodium. Leaching potential was evaluated for tacrolimus and teniposide as well as the vehicles of docetaxel and paclitaxel. Representative concentrations of the drugs in infusion solutions or undiluted were placed into the new generation of VISIV containers and left in contact for up to 24 hours at room temperature. High performance liquid chromatography was used to determine drug concentrations and the presence of plasticizer or other plastic components, if any. Only regular human insulin exhibited any substantial loss of concentration in the polyolefin containers that could be attirbuted to sorption. Other drugs' concentrations were consistent with their stabilities over the test periods. No evidence of leaching of plasiticizer or other plastic components was observed.
Production and characterization of monoclonal antibodies to the protective antigen component of Bacillus anthracis toxin (1988)

Thirty-six monoclonal antibodies to the protective antigen protein of Bacillus anthracis exotoxin have been characterized for affinity, antibody subtype, competitive binding to antigenic regions, and ability to neutralize lethal and edema toxin activities. At least 23 antigenic regions were detected on protective antigen by a blocking, enzyme-linked immunosorbent assay. Two clones, 3B6 and 14B7, competed for a single antigenic region and neutralized the activity of both the lethal toxin in vivo (Fisher 344 rat) and the edema toxin in vitro (CHO cells). These two antibodies blocked the binding of 125I-labeled protective antigen to FRL-103 cells. Our results support the proposal that binding of protective antigen to cell receptors is required for expression of toxicity.
Thermal evolution of impurities in wet chemical silicon oxides (1999)

We have used infrared absorption spectroscopy and x-ray photoelectron spectroscopy to study the thermal evolution (under ultrahigh vacuum conditions) of ultrathin silicon oxide films grown in acid solutions (HCl, HNO3, and H2SO4). We find that adsorbed hydrocarbon contaminants dissociate and become chemically incorporated into the thin oxide as additional silicon oxide, carbide, hydride, and hydroxyl species. These species significantly influence the thermal evolution of the oxide films and persist up to the SiO desorption temperature (850–1000 °C) so that, once formed, these defects will be necessarily present in the final device structure.
Deterministic partitioning techniques for fault diagnosis in scan-based BIST (2002)

A deterministic partitioning technique for fault diagnosis in scan-based BIST is proposed. Properties of high quality partitions for improved fault diagnosis times are identified and low cost hardware implementations of high quality deterministic partitions are outlined. The superiority of the partitions generated by the proposed approach is confirmed through mathematical analysis. Theoretical analyses, worst case bounds, and experimental simulation data all confirm the superiority of the proposed deterministic approaches.
Structural and Functional Properties of an Unusual Internal Fusion Peptide in a Nonenveloped Virus Membrane Fusion Protein (2004)

The avian and Nelson Bay reoviruses are two of only a limited number of nonenveloped viruses capable of inducing cell-cell membrane fusion. These viruses encode the smallest known membrane fusion proteins (p10). We now show that a region of moderate hydrophobicity we call the hydrophobic patch (HP), present in the small N-terminal ectodomain of p10, shares the following characteristics with the fusion peptides of enveloped virus fusion proteins: (i) an abundance of glycine and alanine residues, (ii) a potential amphipathic secondary structure, (iii) membrane-seeking characteristics that correspond to the degree of hydrophobicity, and (iv) the ability to induce lipid mixing in a liposome fusion assay. The p10 HP is therefore predicted to provide a function in the mechanism of membrane fusion similar to those of the fusion peptides of enveloped virus fusion peptides, namely, association with and destabilization of opposing lipid bilayers. Mutational and biophysical analysis suggested that the internal fusion peptide of p10 lacks alpha-helical content and exists as a disulfide-stabilized loop structure. Similar kinked structures have been reported in the fusion peptides of several enveloped virus fusion proteins. The preservation of a predicted loop structure in the fusion peptide of this unusual nonenveloped virus membrane fusion protein supports an imperative role for a kinked fusion peptide motif in biological membrane fusion.
Optical Measurement of Ultrasonic Nanometer Motion of Rough Surface by Two-Wave Mixing in Bi<sub>12</sub>SiO<sub>20</sub> (1995)

Motion of a rough surface of an ultrasonic transducer was measured using an optical interferometer based on photorefractive two-wave mixing in a Bi 12 SiO 20 crystal. The displacement equivalent to noise was 0.10 nm in a band-width of 8.0 MHz. The low-frequency noise was automatically compensated by an adaptive property of the photorefractive grating without an electric feedback loop.
Formation mechanism of 193nm immersion defects and defect reduction strategies (2008)

The formation mechanism of various 193nm immersion-related defects is investigated. The experimental results are reviewed and compared to the simulation results, which enables us to form a clear picture of how the immersion defects are generated. Based on the understanding of defects mechanisms, a series of the defect reduction measures is proposed for the 193nm immersion process.
Rate compatible turbo codes (1995)

Turbo codes can easily be used when two error protection levels are needed. The same encoder and decoder is used to achieve this unequal error protection. This will allow turbo codes to be used for channels where some information bits are more sensitive to errors than others, and at the same time where powerful codes are required (e.g. a mobile satellite channel).
Capacitance cytometry: Measuring biological cells one by one (2000)

Measuring the DNA content of eukaryotic cells is a fundamental task in biology and medicine. We have observed a linear relationship between the DNA content of eukaryotic cells and the change in capacitance that is evoked by the passage of individual cells across a 1-kHz electric field. This relationship is species-independent; consequently, we have developed a microfluidic technique-"capacitance cytometry"-that can be used to quantify the DNA content of single eukaryotic cells and to analyze the cell-cycle kinetics of populations of cells. Comparisons with standard flow cytometry demonstrate the sensitivity of this new technique.
Multiplex Asymmetric PCR-Based Oligonucleotide Microarray for Detection of Drug Resistance Genes Containing Single Mutations in <i>Enterobacteriaceae</i> (2007)

A multiplex asymmetric PCR (MAPCR)-based microarray method was developed for the detection of 10 known extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated AmpC beta-lactamase genes in gram-negative bacteria and for the typing of six important point mutations (amino acid positions 35, 43, 130, 179, 238, and 240) in the bla(SHV) gene. The MAPCR is based on a two-round reaction to promote the accumulation of the single-stranded amplicons amenable for microarray hybridization by employing multiple universal unrelated sequence-tagged primers and elevating the annealing temperature at the second round of amplification. A strategy to improve the discrimination efficiency of the microarray was constituted by introducing an artificial mismatch into some of the allele-specific oligonucleotide probes. The microarray assay correctly identified the resistance genes in both the reference strains and some 111 clinical isolates, and these results were also confirmed for some isolates by direct DNA sequence analysis. The resistance genotypes determined by the microarray correlated closely with phenotypic MIC susceptibility testing. This fast MAPCR-based microarray method should prove useful for undertaking important epidemiological studies concerning ESBLs and plasmid-mediated AmpC enzymes and could also prove invaluable as a preliminary screen to supplement phenotypic testing for clinical diagnostics.