gebhart/patrepeval huggingface dataset:
PBP74, a new member of the mammalian 70-kDa heat shock protein family, is a mitochondrial protein. (1994)

The cloning of a cDNA encoding a new member of the highly conserved mammalian 70-kDa heat shock protein (hsp 70) family termed PBP74 was recently reported. Critical to an understanding of the function of this new hsp 70 is delineating its subcellular localization. Here we use a variety of immunological and biochemical approaches both in vitro and in vivo to demonstrate that PBP74 is imported into and resides in mitochondria. By confocal immunofluorescence microscopy PBP74 is detected in mitochondria, colocalizing with the mitochondrial 60-kDa heat shock protein. To address the inherent problem of serological cross-reactivity among the hsp70 family members, an influenza virus hemagglutinin epitope tag was introduced into the PBP74 cDNA. The epitope-tagged PBP74 protein transiently expressed in L cells localized to mitochondria. Moreover, deletion of the N-terminal 46-amino acid presequence results in a cytosolic localization of the epitope-tagged protein. Cell fractionation studies demonstrated PBP74 in purified mitochondria in a protease-protected location. After coupled transcription-translation the precursor of PBP74 is imported into isolated yeast mitochondria, where it becomes processed to the mature protein. According to a subfractionation of the mitochondria, the imported protein was found to be localized in the matrix space. Import in vitro is time- and temperature-dependent, requires matrix ATP, and is abolished upon depletion of the membrane potential across the mitochondrial inner membrane. Similarly, in mammalian cells PBP74 is synthesized as a pre-protein that requires membrane potential-dependent import into mitochondria for its maturation. Taken together, our data demonstrate that PBP74 is a mammalian mitochondrial hsp70.
Quantification of the effect of Percutaneous Coronary Angioplasty on a stenosed Right Coronary Artery (2010)

Atherosclerosis is the most prevalent cardiovascular disease which affects millions of people globally each year. The development of atheroma is directly connected to the interaction of blood flow with the arterial wall. Therefore, the modeling of this interaction is of critical importance for the study of arterial blood flow mechanics and the progression of atherosclerosis. In this study, we use a patient-specific 3-dimensional model of a stenosed Right Coronary Artery (RCA). A revascularization procedure (Percutaneous Coronary Angiography-PCA) is virtually performed to the stenosed model. The distribution of the Wall Shear Stress (WSS) as well as the wall deformation are compared on the two models after solving the coupled mechanical system using the Finite Element Method (FEM). Results concerning areas of low WSS (0-2 Pa) as well as minimum and maximum wall deformation are presented, quantifying the effect of the revascularization process of the artery.
Space-Time Transmission and Coding for Airborne Radars (2008)

In this paper,dealing with long range airborne radars,it is shown that the simultaneous requirement for wideband waveforms and multiple channels opens the way to new space-time signals and processing techniques,thus improving the detection and classification of multiple ground and air targets.
Peptidomimetics and Peptide Backbone Modifications (2002)

The replacement of the amide bond in a peptide backbone is a widely used form of peptide mimicry. Several of the most common amide bond surrogates, including peptidomimetic work done in this laboratory, and their biological applications are presented in this review.
Poor Cognitive Outcome in Shunt-Responsive Idiopathic Normal Pressure Hydrocephalus (2012)

The novel findings were (a) a significant risk of dementia in iNPH initially responsive to cerebrospinal fluid shunt, (b) cognitive impairment most commonly due to iNPH-related dementia followed by concurrent degenerative brain disease, and (c) a subgroup with dementia related to iNPH without comorbidities.
Human Liver-Derived Stem Cells (2003)

The search for human oval cells or bi-potential stem cells in the human liver is the subject of intensive investigation. Fetal hepatocytes (hepatoblasts) have some proliferative and bipotential capacity, but access to sufficient numbers of cells remains limiting. Candidate stem cells in the adult normal and diseased human liver have been identified using markers such as OV6, CD34, c-kit and NCAM. Lack of stem cell marker specificity however, remains a problem and further, more specific markers are required. The molecular signaling molecules and transcription factors that control proliferation and cell differentiation pathways into hepatocyte or biliary phenotype are beginning to emerge. However, whether any of the current liver stem cell approaches will be converted into effective clinical cell transplantation or gene therapy treatments has yet to be proven.
Abstract 4333: Self-stabilizing ADCs: antibody-drug conjugates prepared with maleimido drug-linkers that catalyze their own thiosuccinimide ring hydrolysis. (2013)

Abstract Many antibody-drug conjugates (ADCs) currently in clinical trials employ maleimide-containing drug-linkers which are conjugated to antibody cysteine residues to form thiosuccinimide linkages. It is now known that these thiosuccinimide linkages can undergo two competing reactions while in plasma: elimination of the maleimide resulting in undesirable loss of drug from the ADC, and hydrolysis of the thiosuccinimide ring resulting in a succinic acid derivative which cannot undergo elimination. Thus, thiosuccinimide ring hydrolysis is a reaction which stabilizes the chemical linkage of the drug to the antibody. We have engineered a new class of drug-linkers which incorporate a basic amino group adjacent to the maleimide, providing intramolecular base catalysis of the ring hydrolysis. This basic group induces the thiosuccinimide to undergo rapid self-catalyzed hydrolysis at neutral pH and room temperature, with complete hydrolysis achieved in less than 2 hours. Once hydrolyzed, the drug-linker is no longer subject to maleimide elimination reactions, preventing loss of drug from the antibody by this mechanism and forming a highly stable ADC. To compare these new drug-linkers to traditional maleimido drug-linkers, a number of in vitro and in vivo studies have been conducted. Results of these studies will be presented to demonstrate a robust improvement in drug-linker stability compared to traditional drug-linker formats. Citation Format: Robert P. Lyon, Jocelyn R. Setter, Tim D. Bovee, Svetlana O. Doronina, Martha E. Anderson, Chris L. Leiske, Peter D. Senter. Self-stabilizing ADCs: antibody-drug conjugates prepared with maleimido drug-linkers that catalyze their own thiosuccinimide ring hydrolysis. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4333. doi:10.1158/1538-7445.AM2013-4333
The Past is Myself: Peter Watkins' <i>Edvard Munch</i> (1973) (2007)

The following article will examine one of those works made in ‘exile’: Watkins’ acclaimed television film, Edvard Munch (1973), which was first transmitted by the Norwegian public television service, NRK (Norsk Riksringkasting), as part of a joint production and telecast with its Swedish counterpart, SR (Sveriges Radio) on 12 and 13 November 1974. At the time, it was generally regarded as Watkins’ greatest film. Ingmar Bergman, after a screening in Sweden, was reported to have remarked ‘the film was made by a genius’ whilst that doyen of English critics, Kenneth Tynan, asserted this of Edvard Munch alongside Culloden and The War Game, following a transmission by BBC TV in March 1976: ‘That makes three masterpieces, more than any other English living director can boast.
Diffraction Kernels for Interactive Sound Propagation in Dynamic Environments (2018)

We present a novel method to generate plausible diffraction effects for interactive sound propagation in dynamic scenes. Our approach precomputes a diffraction kernel for each dynamic object in the scene and combines them with interactive ray tracing algorithms at runtime. A diffraction kernel encapsulates the sound interaction behavior of individual objects in the free field and we present a new source placement algorithm to significantly accelerate the precomputation. Our overall propagation algorithm can handle highly-tessellated or smooth objects undergoing rigid motion. We have evaluated our algorithm's performance on different scenarios with multiple moving objects and demonstrate the benefits over prior interactive geometric sound propagation methods. We also performed a user study to evaluate the perceived smoothness of the diffracted field and found that the auditory perception using our approach is comparable to that of a wave-based sound propagation method.
Web4Groups-a tool for telematic collaboration (2002)

This paper presents the web-based group communication service Web4Groups. First it is shown how the project to develop this service is organized. The work of the project consortium consisting of 12 European partners is described. It is further explained how specific user groups in different areas are involved and how their needs are integrated in the development process. Secondly, the Web4Groups service itself is described with respect to features and technical architecture.
Periodontal therapy alters gene expression of peripheral blood monocytes (2007)

The data suggest that periodontal therapy may alter monocytic gene expression in a manner consistent with a systemic anti-inflammatory effect.
Temporary Primary Frequency Control Support by Variable Speed Wind Turbines— Potential and Applications (2008)

This paper quantifies the capability of providing a short-term excess active power support of a commercial multi-megawatt variable speed wind turbine (VSWT) and generalizes the findings by considering different wind turbine (WT) physical parameters in a wider range from the example case. The paper also identifies some possible applications of it, in particular, in a hydro dominated system. To be able to quantify the system characteristic, a delay model of the studied hydro system is developed. Due to the fact that the initial power surge of a hydro turbine is opposite to that desired, the short-term extra active power support from a wind farm (WF) could be beneficial for a hydro dominated system in arresting the initial frequency fall, which corresponds to an improvement in the system temporary minimum frequency (TMF). The improvements in the TMF are calculated by using both the developed delay model and a detailed model, and the results show good agreement. It is shown that the WT under consideration can provide a 0.1 pu extra active power support for 10 s quite easily which is twice the Hydro-Quebec requirement.
POTENTIAL INVOLVEMENT OF FREE RADICAL REACTIONS IN ULTRAVIOLET LIGHT‐MEDIATED CUTANEOUS DAMAGE* (1987)

Abstract Free radicals are chemical species characterized by an odd number of orbital electrons or by pairs of electrons of similar directional spin isolated singly in separate orbitals. Consequently most of these agents are highly reactive and usually exhibit an extremely short half‐life, although due to steric and resonance effects some exceptions occur. Some radicals and their precursors, such as the diradical O 2 which exists in the triplet state, represent a critical and essential element of normal metabolism of aerobic organisms where, under normal circumstances, controlled reduction of reactive oxygen species occurs via the cytochrome oxidase or cytochrome P‐450 mixed function monooxygenase systems. In addition to reactive oxygen species, organisms may be subjected to a wide‐range of other free radicals or their precursors, including those of both exogenous and endogenous origin. Elaborate defense mechanisms have evolved to avoid cellular damage from these highly reactive species. Enzymes, such as the superoxide dismutase, the glutathione peroxidase/reductase system, and catalase; interactions with conjugated diene systems such as those found in melanins, carotenoids, and tocopherols; and direct reduction by sulphydryl compounds, phenols, and purines represent but a few of these natural defense systems. Despite a strong rationale for considering free radicals as pathologic agents, progress in implicating these agents, or their reactions, in pathologic processes has been arduous. The fore‐most hurdle to providing definitive evidence for free radical involvement rests with the highly transient nature of these species, hardly reaching measurable levels in vivo and thereby making rigorous testing of the hypothesis extremely difficult. Indeed, free radical damage has been studied, for the most part, by indirect means–usually by measurement of known free radical reaction intermediates and products from which free radical involvement is implied. Nevertheless, free radical formation has been shown to occur in UV‐irradiated skin and a considerable body of circumstantial evidence has been amassed that strongly infers that these agents, or reactions initiated by them, are responsible for at least some of the deleterious effects of UV upon skin.
Anatomical and physical barriers to tumor targeting with oncolytic adenoviruses in vivo. (2009)

Despite promising results in preclinical studies, the therapeutic efficacy of oncolytic adenoviruses (Ads) has not been reproduced in clinical trials in patients with cancer. Recent studies have identified several obstacles that limit the therapeutic efficacy of oncolytic Ads. These obstacles include the lack of a primary Ad attachment receptor on tumor cells in situ, as well as innate host defense mechanisms that recognize Ads as pathogens and act to facilitate their elimination. This review focuses on anatomical barriers inside solid tumors that limit Ad transduction, and specifically barriers associated with several factors: (i) the mechanisms of tumor vascularization and the metabolic microenvironment; (ii) the tumor stroma, which tightly surrounds nests of malignant cells; and (iii) the epithelial phenotype of cancer cells, particularly the trapping of viral receptors in tight and adherens junctions. Potential strategies to overcome these obstacles are also presented. Because published data on the physical barriers to Ad transduction of tumors in situ are limited, studies with other macromolecules used in tumor therapy are also discussed.
Stereochemical Aspects of Tellurium Complexes with Sulfur Ligands: Molecular Compounds and Supramolecular Associations (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStereochemical Aspects of Tellurium Complexes with Sulfur Ligands: Molecular Compounds and Supramolecular AssociationsI. Haiduc, R. B. King, and M. G. NewtonCite this: Chem. Rev. 1994, 94, 2, 301–326Publication Date (Print):March 1, 1994Publication History Published online1 May 2002Published inissue 1 March 1994https://pubs.acs.org/doi/10.1021/cr00026a002https://doi.org/10.1021/cr00026a002research-articleACS PublicationsRequest reuse permissionsArticle Views533Altmetric-Citations103LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Adeno-associated Virus and Lentivirus Pseudotypes for Lung-directed Gene Therapy (2004)

The enthusiasm for cystic fibrosis gene therapy that attended the initial cloning of the gene and in vitro correction of the genetic defect eventually diminished as we learned more about the limitations of vector technologies that were available in the 1980s and 1990s. Substantial progress has been made, however, over the last 5 years in developing second- and third-generation vector constructs that should be more useful in achieving gene transfer to the lung for the treatment of pulmonary diseases such as cystic fibrosis.
Error-corrected large-signal waveform measurement system combining network analyzer and sampling oscilloscope capabilities (1990)

A large-signal automatic stepped CW waveform measurement system for nonlinear device characterization is presented that combines the high accuracy of a vector network analyzer with the waveform measurement capabilities of a sampling oscilloscope. A large-signal error model and a corresponding coaxial calibration procedure are proposed to describe the systematic errors of the measurement setup. The error parameters and the correction algorithm are independent of the properties of the RF generator. System accuracy is investigated by Schottky diode verification measurements with different offsets from the reference plane. GaAs MESFET reflection and transmission response measurements with error correction extended to the planar device under test (DUT) reference planes are given.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Progenitor Cell Therapy in Patients With Critical Limb Ischemia Without Surgical Options (2008)

Pioneering clinical studies report promising results for progenitor cell-based therapies for chronic limb ischemia, but no definite proof is available because the clinical studies thus far have been small and lacked double-blinded controls. Larger, randomized, blinded, placebo-controlled trials to investigate the potential clinical effects of bone marrow progenitor cell administration in patients with critical limb ischemia are needed.
Medical Implications of Obesity in Horses—Lessons for Human Obesity (2009)

There is growing recognition that obesity is common and represents a significant detriment to the health of companion animals in a manner similar to that by which it is affecting the human population. As is the case for other species, obesity appears to promote insulin resistance in horses and it is through this pathophysiological process that many of the adverse medical consequences of obesity are being characterized. Equine medical conditions that have been described in the context of obesity and insulin resistance differ from those in humans. Chronic human conditions that have been attributed to obesity and insulin resistance, such as atherosclerosis and diabetes mellitus, are rarely described in obese horses. Significant current interest is centered on the recognition that insulin resistance plays a role in the pathogenesis of laminitis, a potentially severe and debilitating cause of lameness in the equine species. Other equine medical conditions that are more likely in obese, insulin-resistant individuals include hyperlipemia (hepatic lipidosis) and developmental orthopedic disease (osteochondrosis). Pituitary pars intermedia dysfunction (equine Cushing's syndrome) represents another common endocrinopathic condition of older horses associated with insulin resistance. This review presents an introductory overview of the present understanding of obesity and insulin resistance and how these conditions may be associated with disease conditions in horses.
A new ARQ-scheme for multicast satellite communication (1993)

More and more satellite networks with a huge number of stations are built up. Because of the broadcast capability these networks are predestined for multicast applications. Many of them require an error free transmission of data which cannot be guaranteed by using only a forward error correction technique. Therefore, automatic repeat request schemes must be applied which retransmit erroneously received data. The most efficient technique is selective repeat: besides the case of a buffer overflow at the receiver's site only erroneous frames are retransmitted. So each incorrect received frame must be repeated (separately) in multicast environments. The authors propose a new scheme which is especially designed for a point-to-multipoint scenario. In their 'XOR-protocol' one retransmission corrects several erroneously transmitted frames at once. >
P59 (FK506 binding protein 59) interaction with heat shock proteins is highly conserved and may involve proteins other than steroid receptors (1993)

P59 [also known as FK506 binding protein 59 (FKBP59) or heat shock protein 56 (hsp56)] and heat shock proteins 90 and 70 (hsp90 and hsp70) associate with steroid receptors and are believed to maintain the receptors in an inactive state. Recently, we showed that p59 purified from human lymphocytes is an immunophilin (FKBP59) which binds both FK506 and rapamycin. It was also demonstrated that immunosuppressant-FKBP59 complexes associate with hsp90, hsp70, and the glucocorticoid receptor [Tai, P.-K. K., Albers, M. W., Chang, H., Faber, L. E., & Schreiber, S. L. (1992) Science 256, 1315-1318]. Here we provide evidence that rabbit uterine p59 also binds FK506 and rapamycin and that p59 or its homologue is associated with nontransformed progesterone receptors of rabbit uterus and chicken oviduct. This suggests that the immunophilin-heat shock protein-steroid receptor interaction is ubiquitous and not limited to immune systems. A FKBP59 homologue complexed with hsp90-hsp70 was also detected in yeast, which suggests that the immunophilin-heat shock protein association has been evolutionarily conserved. In addition, we found that the FKBP59-hsp complexes are more complicated than previously thought, involving other proteins such as actin and a 63-kDa protein, p63. The association of p63 to the p59 complex was inhibited by FK506 and rapamycin, suggesting that p63 could be a potential target for the immunosuppressive actions of these two drugs.(ABSTRACT TRUNCATED AT 250 WORDS)
Superior Duplex DNA Strand Invasion by Acridine Conjugated Peptide Nucleic Acids (2003)

DNA helix invasion by P-loop forming peptide nucleic acids (PNAs) is extremely sensitive to increased ionic strength as this stabilizes the DNA duplex. To address this, the DNA intercalator 9-aminoacridine was conjugated to helix invading PNAs, and the duplex DNA binding efficiency of such constructs was measured at different ionic strength conditions by electrophoretic mobility shift analysis. Remarkably, at physiogically relevant ionic strength (140 mM K+/10 mM Na+, 2 mM Mg2+), acridine conjugated PNAs showed 20-150-fold superior binding to a cognate sequence target as compared to the conventional PNAs. This enhancement occurred without compromising the sequence specificity of binding. Thus, simply conjugating the DNA intercalator 9-aminoacridine to PNA represents a major step toward the development of helix invading constructs for in vivo applications such as gene targeting.