gebhart/patrepeval huggingface dataset:
Virus-like particle display of HER2 induces potent anti-cancer responses (2017)

Overexpression of human epidermal growth factor receptor-2 (HER2) occurs in 20-30% of invasive breast cancers. Monoclonal antibody therapy is effective in treating HER2-driven mammary carcinomas, but its utility is limited by high costs, side effects and development of resistance. Active vaccination may represent a safer, more effective and cheaper alternative, although the induction of strong and durable autoantibody responses is hampered by immune-tolerogenic mechanisms. Using a novel virus-like particle (VLP) based vaccine platform we show that directional, high-density display of human HER2 on the surface of VLPs, allows induction of therapeutically potent anti-HER2 autoantibody responses. Prophylactic vaccination reduced spontaneous development of mammary carcinomas by 50%-100% in human HER2 transgenic mice and inhibited the growth of HER2-positive tumors implanted in wild-type mice. The HER2-VLP vaccine shows promise as a new cost-effective modality for prevention and treatment of HER2-positive cancer. The VLP platform may represent an effective tool for development of vaccines against other non-communicable diseases.
Olefin copolymerization with metallocene catalysts. IV. Metallocene/methylaluminoxane catalyzed olefin teropolymerization (1991)

Abstract Ethylene, propylene, and ethylidene norbornene (D) were terpolymerized by Et [Ind] 2 ZrCl 2 /MAO in solution and supported on silica. The presence of D decreases the rate of polymerization. EPDM containing between 3 and 22 wt % of D were synthesized which have narrow MW distribution. The supported catalyst produces EPDM with E/P ratio which are similar to the ratio in the feed.
Synthesis and biological activities of pseudopeptide analogs of the C-terminal heptapeptide of cholecystokinin. On the importance of the peptide bonds (1987)

A series of pseudopeptide analogues of the C-terminal heptapeptide of cholecystokinin in which each peptide bond, one at a time, has been replaced by a CH2NH bond were synthesized: Z-Tyr(SO3-)-Nle-Gly-Trp-Nle-Asp psi-(CH2NH)Phe-NH2 (1), Z-Tyr(SO3-)-Nle-Gly-Trp-Nle psi (CH2NH)Asp-Phe-NH2 (2), Z-Tyr(SO3-)-Nle-Gly-Trp psi-(CH2NH)Nle-Asp-Phe-NH2 (3), Z-Tyr(SO3-)-Nle-Gly psi(CH2NH)Trp-Nle-Asp-Phe-NH2 (4), Z-Tyr(SO3-)-Nle psi-(CH2NH)Gly-Trp-Nle-Asp-Phe-NH2 (5), Z-Tyr(SO3-)-Met-Gly-Trp-Nle-Asp psi (CH2NH)Phe-NH2 (6), Z-Tyr-(SO3-)-Met-Gly-Trp-Nle psi (CH2NH)Asp-Phe-NH2 (7), Z-Tyr(SO3-)-Met-Gly-Trp psi (CH2NH)Nle-Asp-Phe-NH2 (8). These derivatives were studied for their ability to stimulate amylase release from rat pancreatic acini and to inhibit the binding of labeled CCK-9 to rat pancreatic acini and to guinea pig brain membrane CCK receptors. They were compared to the potent CCK-8 analogue Boc-Asp-Tyr(SO3-)-Nle-Gly-Trp-Nle-Asp-Phe-NH2. All of these pseudopeptides were able to stimulate amylase secretion with the same efficacy as CCK-8 but with varying potencies. These compounds were also potent in inhibiting the binding of labeled CCK-9 to CCK receptors from rat pancreatic acini and from guinea pig brain membranes.
Sensor-Based Exploration: The Hierarchical Generalized Voronoi Graph (2000)

The hierarchical generalized Voronoi graph (HGVG) is a new roadmap developed for sensor-based exploration in unknown environments. This paper defines the HGVG structure: a robot can plan a path between two locations in its work space or configuration space by simply planning a path onto the HGVG, then along the HGVG, and finally from the HGVG to the goal. Since the bulk of the path planning occurs on the one-dimensional HGVG, motion planning in arbitrary dimensioned spaces is virtually reduced to a one-dimensional search problem. A bulk of this paper is dedicated to ensuring the HGVG is sufficient for motion planning by demonstrating the HGVG (with its links) is an arc-wise connected structure. All of the proofs in this paper that lead toward the connectivity result focus on a large subset of spaces in R 3 , but wherever possible, results are derived in R m . In fact, under a strict set of conditions, the HGVG (the GVG by itself) is indeed connected, and hence sufficient for motion planning. The chief advantage of the HGVG is that it possesses an incremental construction procedure, described in a companion paper, that constructs the HGVG using only line-of-sight sensor data. Once the robot constructs the HGVG, it has effectively explored the environment, because it can then use the HGVG to plan a path between two arbitrary configurations.
Molecular Mechanisms of Epidermal Growth Factor Receptor (EGFR) Activation and Response to Gefitinib and Other EGFR-Targeting Drugs (2006)

The epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases, including EGFR, HER2/erbB2, and HER3/erbB3, is an attractive target for antitumor strategies. Aberrant EGFR signaling is correlated with progression of various malignancies, and somatic tyrosine kinase domain mutations in the EGFR gene have been discovered in patients with non-small cell lung cancer responding to EGFR-targeting small molecular agents, such as gefitinib and erlotinib. EGFR overexpression is thought to be the principal mechanism of activation in various malignant tumors. Moreover, an increased EGFR copy number is associated with improved survival in non-small cell lung cancer patients, suggesting that increased expression of mutant and/or wild-type EGFR molecules could be molecular determinants of responses to gefitinib. However, as EGFR mutations and/or gene gains are not observed in all patients who respond partially to treatment, alternative mechanisms might confer sensitivity to EGFR-targeting agents. Preclinical studies showed that sensitivity to EGFR tyrosine kinase inhibitors depends on how closely cell survival and growth signalings are coupled with EGFR, and also with HER2 and HER3, in each cancer. This review also describes a possible association between EGFR phosphorylation and drug sensitivity in cancer cells, as well as discussing the antiangiogenic effect of gefitinib in association with EGFR activation and phosphatidylinositol 3-kinase/Akt activation in vascular endothelial cells.
Implementation of high speed Viterbi detectors (1999)

The normal Viterbi architecture and a radix 4 architecture are compared with a parallel ACS version using a latch based storage element. Results obtained using an eight-state EPR4 Viterbi detector show that parallel ACS architectures can provide a high level of performance with modest area requirements.
Fusion Research of Electrical Tomography with Other Sensors for Two-phase Flow Measurement (2012)

The two-phase flow widely exists in the nature and industrial processes. The measurement of two-phase flows, including gas/solids, gas/liquid and liquid/liquid flows, is still challenging. Fusions of electrical tomography with conventional sensors provide possibilities to improve two-phase flow accurate measurement. In this paper, fusions of (1) electrical resistance tomography (ERT) with electromagnetic (EM) flowmeter, (2) electrical capacitance tomography (ECT) with ERT and (3) ECT with electrostatic sensor are introduced. Some research results of fusion methods are presented and discussed. This paper can provide the theoretical support for the multi-sensor fusion for two-phase flow measurement.
Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier (2011)

In past two decades poly lactic-co-glycolic acid (PLGA) has been among the most attractive polymeric candidates used to fabricate devices for drug delivery and tissue engineering applications. PLGA is biocompatible and biodegradable, exhibits a wide range of erosion times, has tunable mechanical properties and most importantly, is a FDA approved polymer. In particular, PLGA has been extensively studied for the development of devices for controlled delivery of small molecule drugs, proteins and other macromolecules in commercial use and in research. This manuscript describes the various fabrication techniques for these devices and the factors affecting their degradation and drug release.
How To Write Shared Libraries (2005)

Today, shared libraries are ubiquitous. Developers use them for multiple reasons and create them just as they would create application code. This is a problem, though, since on many platforms some additional techniques must be applied even to generate decent code. Even more knowledge is needed to generate optimized code. This paper introduces the required rules and techniques. In addition, it introduces the concept of ABI (Application Binary Interface) stability and shows how to manage it. 1 Preface For a long time, programmers collected commonly used code in libraries so that code could be reused. This saves development time and reduces errors since reused code only has to be debugged once. With systems running dozens or hundreds of processes at the same time reuse of the code at link-time solves only part of the problem. Many processes will use the same pieces of code which they import for libraries. With the memory management systems in modern operating systems it is also possible to share the code at run-time. This is done by loading the code into physical memory only once and reusing it in multiple processes via virtual memory. Libraries of this kind are called shared libraries. The concept is not very new. Operating system designers implemented extensions to their system using the infrastructure they used before. The extension to the OS could be done transparently for the user. But the parts the user directly has to deal with created initially problems.
Melanoma-reactive human cytotoxic T lymphocytes derived from skin biopsies of delayed-type hypersensitivity reactions induced by injection of an autologous melanoma cell line. (1997)

The expression by melanomas of multiple antigens that are recognized by specific MHC class I-restricted CTLs has been clearly demonstrated. The goal of many immunotherapy protocols being developed is, therefore, the induction and/or augmentation of CTLs specific for such antigens. One approach has been to immunize using irradiated autologous melanoma cells. Responses to this type of immunization and others are often subsequently measured by delayed-type hypersensitivity (DTH) reactions. The aim of this work was to characterize whether specific CTL responses occur at such DTH sites. Cutaneous DTH reactions were observed following injection of irradiated autologous melanoma cells expressing known tumor antigens. We isolated lymphocytes from biopsies of DTH reaction sites and could measure melanoma-specific CTL activity after 2-3 weeks of culture. The T-cell receptor-Vbeta repertoire of the cultured lymphocytes, assessed by flow cytometry, was highly skewed in both the CD4(+) and CD8(+) T-cell subsets. The repertoires were different among cultures derived from independent biopsies of simultaneous or subsequent DTH reaction sites and very different to that of fresh peripheral blood lymphocytes (PBLs) or PBLs cultured under the same conditions. No particular T-cell expansions dominated several DTH reaction sites, nor could they be detected in PBLs. It appears that T-cell responses to this type of immunization may be limited to the local microenvironment. Establishing the value of DTH reactions in determining levels of systemic antitumor immunity requires further investigation; however, such reactions may indicate a patient's competence to mount an antitumor immune response and enable the isolation of tumor-specific CTLs for use in tumor antigen identification.
Experimental observations of the depth-discrimination properties of scanning microscopes (1981)

We present experimental confirmation of the predicted improved depth-discrimination properties of confocal microscopy in which detail outside the focal plane is rejected from the image. This optical sectioning is of direct importance to the microscopy of thick biological tissue. It is shown that detail that is imaged efficiently in a confocal microscope will be in focus.
Reversible Oxidation of Graphene Through Ultraviolet/Ozone Treatment and Its Nonthermal Reduction through Ultraviolet Irradiation (2014)

We present alternative methods to oxidize a graphene layer through ultraviolet (UV)/ozone (O3)-treatment, resulting in chemically homogeneous graphene oxide (GO), and then to reduce GO through UV-irradiation. Both UV/O3-treatment and UV-irradiation were performed at room temperature in atmospheric pressure for only several minutes and did not involve any wet chemical treatments. The quantity of doped oxygen, determined using X-ray photoelectron spectroscopy, increased after oxidation and decreased after reduction. The quantity of doped oxygen reached its maximum, which was around 20% (approximately one oxygen atom in every five or six carbon atoms) after performing UV/O3-treatment for 6 to 10 min. Conducting UV/O3-treatment for around 6 or 10 min resulted in a chemically homogeneous GO surface with only oxygen epoxide groups on the graphene surface. Performing UV/O3-treatment beyond 15 min as well as multiple turns of UV/O3-treatment could lead to the formation of defects and carbonyl groups on graphene lattice. The oxygen quantity gradually decreased after conducting 6 min UV-irradiation several times, indicating that the resulting GO was successfully reduced. How the doped oxygen atoms distributed on graphene surface was directly investigated using scanning tunneling microscopy. Moreover, changes in electrical properties of three identical single-layer graphene field-effect transistors (G-FETs) after being oxidized through UV/O3-treatment were investigated. The electron mobility of G-FETs decreased after oxidation; however, it recovered after irradiating the oxidized G-FETs with UV-lights, indicating that GO was successfully reduced nonthermally through UV-irradiation. The reversibility in electron mobility was confirmed even after conducting redox processes twice. Furthermore, the reversibility of oxidation was also verified from the graphene lattice disorder point of view using Raman spectroscopy. We concluded that UV/O3-treatment produced chemically homogeneous GO that is nonthermally reversible through UV-irradiation, and changes in the electron mobility were nonthermally reversible also.
New coupling method for GaAs-laser–fibre coupling (1975)

A new method for coupling a diode laser to an optical fibre is described. This uses a cylindrical glass fibre as a focusing element. Optical feedback from the fibre endface has been found to have an influence on the laser output power. As a result, the light power launched into a single-mode fibre was higher than that obtainable from the laser during normal operation.
Common precursors for neural and mesectodermal derivatives in the cephalic neural crest (1991)

The cephalic neural crest (NC) of vertebrate embryos yields a variety of cell types belonging to the neuronal, glial, melanocytic and mesectodermal lineages. Using clonal cultures of quail migrating cephalic NC cells, we demonstrated that neurons and glial cells of the peripheral nervous system can originate from the same progenitors as cartilage, one of the mesectodermal derivatives of the NC. Moreover, we obtained evidence that the migrating cephalic NC contains a few highly multipotent precursors that are common to neurons, glia, cartilage and pigment cells and which we interprete as representative of a stem cell population. In contrast, other NC cells, although provided with identical culture conditions, give rise to clones composed of only one or some of these cell types. These cells thus appear restricted in their developmental potentialities compared to multipotent cells. It is therefore proposed that, in vivo, the active proliferation of pluripotent NC cells during the migration process generates distinct subpopulations of cells that become progressively committed to different developmental fates.
Does a Statistically Significant Survival Benefit of Erlotinib Plus Gemcitabine for Advanced Pancreatic Cancer Translate Into Clinical Significance and Value? (2007)

may not reflect the resistance of most primary human malignancies and are therefore not likely to be predictive of clinical responses. Moreover, it is possible that the combination of chemotherapy and TRAIL agonistic MAbs may result in unanticipated toxicities in man. The major concern with all therapies targeting the TRAIL pathway remains hepatotoxicity. Prior to entering the clinic, it is important to assess the potential hepatotoxicity of combinations of TRAIL agonistic MAbs and chemotherapy drugs in models that most closely mimic the in vivo situation. We currently lack a good preclinical model with which to assess such combinations. Mouse models may not be adequate since there is only one TRAIL receptor in this species.
Detection of enzyme activity in orthotopic murine breast cancer by fluorescence lifetime imaging using a fluorescence resonance energy transfer–based molecular probe (2011)

Cancer-related enzyme activity can be detected noninvasively using activatable fluorescent molecular probes. In contrast to "always-on" fluorescent molecular probes, activatable probes are relatively nonfluorescent at the time of administration due to intramolecular fluorescence resonance energy transfer (FRET). Enzyme-mediated hydrolysis of peptide linkers results in reduced FRET and increase of fluorescence yield. Separation of signal from active and inactive probe can be difficult with conventional intensity-based fluorescence imaging. Fluorescence lifetime (FLT) measurement is an alternative method to detect changes in FRET. Thus, we investigate FLT imaging for in vivo detection of FRET-based molecular probe activation in an orthotopic breast cancer model. Indeed, the measured FLT of the enzyme-activatable molecular probe increases from 0.62 ns just after injection to 0.78 ns in tumor tissue after 4 h. A significant increase in FLT is not observed for an always-on targeted molecular probe with the same fluorescent reporter. These results show that FLT contrast is a powerful addition to preclinical imaging because it can report molecular activity in vivo due to changes in FRET. Fluorescence lifetime imaging exploits unique characteristics of fluorescent molecular probes that can be further translated into clinical applications, including noninvasive detection of cancer-related enzyme activity.
Dichloroacetate and cerebral ischaemia therapeutics (1999)

Brain ischaemia is a major medical problem which totally lacks meaningful therapeutic options. A drug that reduces morbidity and mortality associated with head injury and stroke would constitute a major medical breakthrough. Although many mechanistic approaches have been evaluated clinically for both stroke and head injury, none have yet to be proven successful. Dichloroacetate (DCA, Ceresine) is a small molecule that activates pyruvate dehydrogenase (PDH) and crosses the blood-brain barrier. PDH activation reduces neurotoxic lactic acidosis which always accompanies brain ischaemia. DCA shows substantial efficacy in a variety of models of stroke, pre-stroke, head or spinal cord injury. Agents that lower cerebral lactic acidosis have not yet been clinically evaluated in head injury and stroke, although DCA has been shown clinically to reduce ambient lactate concentrations in patients with such conditions. DCA has also been shown to be well-tolerated in these patients, and unlike many halogenated molecules, is not mutagenic. Since elevated brain lactate is correlated with poor outcome in both preclinical and clinical studies, an agent such as DCA may prove to reduce the brain injury associated with these disorders. Potential clinical applications of DCA include stroke, head injury, spinal cord injury, and chronic disorders such as congenital lactic acidosis (CLA) and mitochondrial lactic acidosis and stroke-like syndrome (MELAS).
Molecular analysis of tetracycline resistance in Salmonella enterica subsp. enterica serovars Typhimurium, Enteritidis, Dublin, Choleraesuis, Hadar and Saintpaul: construction and application of specific gene probes (2000)

A total of 65 epidemiologically unrelated tetracycline-resistant isolates of the six Salmonella enterica subsp. enterica (Salm.) serovars Dublin, Choleraesuis, Typhimurium, Enteritidis, Hadar and Saintpaul were investigated for the presence of tetracycline resistance genes. For this, specific gene probes of the tetracycline resistance genes (tet) of the hybridization classes A, B, C, D, E and G were constructed by cloning PCR-amplified internal segments of the respective tet structural genes. These gene probes were sequenced and used in hybridization experiments with plasmid DNA or endonuclease digested whole cell DNA as targets. Only tet(A) genes were detected on plasmids in all Salm. Dublin isolates as well as in single isolates of Salm. Choleraesuis and Salm. Typhimurium. Genes of the hybridization classes B, C, D and G, but also in some cases those of class A, were located in the chromosomal DNA of the corresponding Salmonella isolates. Restriction fragment length polymorphisms (RFLPs) of tet gene carrying fragments were detected in chromosomally tetracycline-resistant isolates. These RFLPs might represent valuable additional tools for the identification and characterization of tetracycline-resistant Salmonella isolates.
A methodology for true orthorectification of large-scale urban aerial images and automatic detection of building occlusions using digital surface model (2004)

In urban area with high buildings, conventional orthorectification using digital terrain model (DTM) cannot meet the requirements of true orthoimage generation. Using digital surface model (DSM) integrating from DTM and digital building model (DBM), we propose a methodology in the study to automatically orthorectify large-scale urban aerial image and detect building occlusions for true orthoimage generation. Principle behind the methodology is the optical characteristic of buildings in photogrammetry, in which roof and root sharing the same coordinate are featured with different distances to imagery center. Therefore, in practical operation, we first compute the distances of input image pixels to imagery center and their coordinates is output orthoimage. A data matrix is used to remember the distances and coordinated. Then we compare the coordinates in the matrix. When pixels have the same coordinate, the one with the longest distance represents building root. For true orthoimage generation, only gray value of the roof need to convert into the output image and others are treated as occluded area. By creating an index image, the occluded area can be recorded for next step processing, such as refilling form neighboring orthoimages. Therefore, using a data, matrix and an index image, we are able to automatically orthorectify large-scale urban aerial images and detect building occlusions for true orthoimage generation, provided that the digital surface model is available and the overlap of neighboring image is large enough to ensure 100% visibility of the occluded area.
Efficient “Pure” Blue OLEDs Employing Tetradentate Pt Complexes with a Narrow Spectral Bandwidth (2014)

Efficient deep-blue-emitting tetradentate platinum complexes with a narrow spectral bandwidth are presented, which demonstrate CIEx ≈ 0.15 and CIEy < 0.1. Ultimately, an organic light-emitting diode (OLED) with 24.8% peak external quantum efficiency and CIE coordinates of (0.147, 0.079) is fabricated using PtON7-dtb.