gebhart/patrepeval huggingface dataset:
Household Transmission of the 2009 Pandemic A/H1N1 Influenza Virus: Elevated Laboratory‐Confirmed Secondary Attack Rates and Evidence of Asymptomatic Infections (2010)

Household transmission of pH1N1 may be substantially greater than previously estimated, especially in association with clinical presentations that include gastrointestinal complaints. Approximately 10% of pH1N1 infections acquired in the household may be asymptomatic.
Selective Syntheses of [7]–[12]Cycloparaphenylenes Using Orthogonal Suzuki–Miyaura Cross-Coupling Reactions (2012)

The divergent, selective syntheses of [7]-[12]cycloparaphenylenes have been accomplished utilizing sequential, orthogonal Suzuki-Miyaura cross-coupling reactions from two late-stage intermediates. Quantum yields decrease dramatically as cycloparaphenylene size decreases, highlighting the unique photophysical behavior of the smaller cycloparaphenylenes.
Different Forms of Human Cystatin C (1990)

Two isoelectric forms of human cystatin C with pI 9.2 and 7.8 have been isolated from urine of patients with different nephrological disorders. Treatment of both forms with alkaline phosphatase revealed that the difference between them is not due to the phosphorylation of some amino-acid residue. Further purification of cystatin C with pI 9.2 by hydrophobic chromatography and N-terminal sequencing showed that it consists predominantly of the full-length form of cystatin C with the N-terminal sequence SSPG-. Cystatin C with pI 7.8 was separated into two peaks. The first represented a pure form truncated by an octapeptide and beginning with the N-terminal sequence LVGG-. The second was a mixture containing 33% of the first peak and 66% of a truncated form with the N-terminal sequence VGGP-. Inhibitory activity of the full-length cystatin C and the pure truncated form has been measured against cathepsins B, H and L and show no significant differences in Ki values. These results further support the proposed mechanism of interaction of cysteine proteinases with their inhibitors cystatins (Bode, W., Engh, R., Musil, D., Thiele, U., Huber, R., Karshikow, A., Brzin, J., Kos, J. & Turk, V. (1988) EMBO J. 7, 2593-2599).
Axial-beam on-column absorption detection for open tubular capillary liquid chromatography (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAxial-beam on-column absorption detection for open tubular capillary liquid chromatographyXiaobing. Xi and Edward S. YeungCite this: Anal. Chem. 1990, 62, 15, 1580–1585Publication Date (Print):August 1, 1990Publication History Published online1 May 2002Published inissue 1 August 1990https://pubs.acs.org/doi/10.1021/ac00214a008https://doi.org/10.1021/ac00214a008research-articleACS PublicationsRequest reuse permissionsArticle Views101Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Lower extremities: MR angiography with a unilateral telescopic phased-array coil. (1995)

In a comparison of conventional and magnetic resonance (MR) angiography of the lower extremities, MR imaging was performed with extremity and body coils and a unilateral six-coil telescopic phased-array coil. In one of seven volunteers, average signal-to-noise ratio was 140% higher with the latter (p < .01), only three of five stations were necessary, and examination time was decreased 50%. In the four patients, correlation in findings was 100%.
Optimal gait and form for animal locomotion (2009)

We present a fully automatic method for generating gaits and morphologies for legged animal locomotion. Given a specific animal's shape we can determine an efficient gait with which it can move. Similarly, we can also adapt the animal's morphology to be optimal for a specific locomotion task. We show that determining such gaits is possible without the need to specify a good initial motion, and without manually restricting the allowed gaits of each animal. Our approach is based on a hybrid optimization method which combines an efficient derivative-aware spacetime constraints optimization with a derivative-free approach able to find non-local solutions in high-dimensional discontinuous spaces. We demonstrate the effectiveness of this approach by synthesizing dynamic locomotions of bipeds, a quadruped, and an imaginary five-legged creature.
Studies on the mechanism of membrane fusion: site-specific mutagenesis of the hemagglutinin of influenza virus. (1986)

Oligonucleotide-directed mutagenesis of a cDNA encoding the hemagglutinin of influenza virus has been used to introduce single base changes into the sequence that codes for the conserved apolar "fusion peptide" at the amino-terminus of the HA2 subunit. The mutant sequences replaced the wild-type gene in SV40-HA recombinant virus vectors, and the altered HA proteins were expressed in simian cells. Three mutants have been constructed that introduce single, nonconservative amino acid changes in the fusion peptide, and three fusion phenotypes were observed: substitution of glutamic acid for the glycine residue at the amino-terminus of HA2 abolished all fusion activity; substitution of glutamic acid for the glycine residue at position 4 in HA2 raised the threshold pH and decreased the efficiency of fusion; and, finally, extension of the hydrophobic stretch by replacement of the glutamic acid at position 11 with glycine yielded a mutant protein that induced fusion of erythrocytes with cells with the same efficiency and pH profile as the wild-type protein. However, the ability of this mutant to induce polykaryon formation was greatly impaired. Nevertheless, all the mutant proteins underwent a pH-dependent conformational change and bound to liposomes. These results are discussed in terms of the mechanism of HA-induced membrane fusion.
An integrated life-cycle model for software maintenance (1988)

An integrated life-cycle model is presented for use in a software maintenance environment. The model represents information about the development and maintenance of software systems, emphasizing relationships between different phases of the software life cycle. It provides the basis for automated tools to assist maintenance personnel in making changes to existing software systems. The model is independent of particular specification, design, and programming languages because it represents only certain 'basic' semantic properties of software systems: control flow, data flow, and data structure. The software development processes by which one phase of the software life cycle is derived from another are represented by graph rewriting rules, which indicate how various components of a software system have been implemented. This approach permits analysis of the basic properties of a software system throughout the software life cycle. Examples are given to illustrate the integrated software life-cycle model during evolution.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Optimal Power Control, Scheduling, and Routing in UWB Networks (2004)

Ultra-wideband (UWB) is an emerging wireless physical layer technology that uses a very large bandwidth. We are interested in finding the design objectives of the medium access [medium-access control (MAC), namely, power control and scheduling] and routing protocols of a multihop, best-effort, UWB network. Our objective is to maximize flow rates (more precisely, log-utility of flow rates) given node power constraints. The specificity of UWB is expressed by the linear dependence between rate and signal-to-noise ratio at the receiver. It is known that, in wireless networks, different routing strategies can imply differences in MAC protocol design. Hence, we search for the jointly optimal routing, scheduling and power control. We find that the optimal solution is characterized by the following. 1) When data is being sent over a link, it is optimal to have an exclusion region around the destination, in which all nodes remain silent during transmission, whereas nodes outside of this region can transmit in parallel, regardless of the interference they produce at the destination. Additionally, the source adapts its transmission rate according to the level of interference at the destination due to sources outside of the exclusion region. 2) The optimal size of this exclusion region depends only on the transmission power of the source of the link, and not on the length of the link nor on positions of nodes in its vicinity. 3) Each node in a given time slot either sends data at the maximum power, or does not send at all. As for the routing, we restrict ourselves to a subset of routes where on each successive hop we decrease the distance toward the destination, and we show that 4) relaying along a minimum energy and loss route is always better than using longer hops or sending directly, which is not obvious since we optimize rate and not power consumption. Finally 5), the design of the optimal MAC protocol is independent of the choice of the routing protocol. For narrowband networks, 2), 4), and 5) do not hold, which shows that the design of an UWB network should be addressed in a different way than for narrowband. Our technical approach is based on expressing the design requirements as a mathematical optimization problem. We solve it exactly for simple networks on a line and approximately on random topologies in a plane with up to 50 nodes with various power constraints, traffic matrices, and mobility parameters.
Reduction of Dehydroascorbate to Ascorbate by the Selenoenzyme Thioredoxin Reductase (1997)

Recycling of ascorbate from its oxidized forms is essential to maintain stores of the vitamin in human cells. Whereas reduction of dehydroascorbate to ascorbate is thought to be largely GSH-dependent, we reconsidered the possibility that the selenium-dependent thioredoxin system might contribute to ascorbate regeneration. We found that purified rat liver thioredoxin reductase functions as an NADPH-dependent dehydroascorbate reductase, with an apparent Km of 2. 5 mM for dehydroascorbate, and a kcat of 90 min-1. Addition of 2.8 microM purified rat liver thioredoxin lowered the apparent Km to 0.7 mM, without affecting the turnover (kcat of 71 min-1). Since thioredoxin reductase requires selenium, we tested the physiologic importance of this enzyme for dehydroascorbate reduction in livers from control and selenium-deficient rats. Selenium deficiency lowered liver thioredoxin reductase activity by 88%, glutathione peroxidase activity by 99%, and ascorbate content by 33%, but did not affect GSH content. NADPH-dependent dehydroascorbate reductase activity due to thioredoxin reductase, on the basis of inhibition by aurothioglucose, was decreased 88% in dialyzed liver cytosolic fractions from selenium-deficient rats. GSH-dependent dehydroascorbate reductase activity in liver cytosol was variable, but typically 2-3-fold that of NADPH-dependent activity. These results show that the thioredoxin system can reduce dehydroascorbate, and that this function is required for maintenance of liver ascorbate content.
High density packaging of flash memory (2002)

The next innovation in three-dimensional (3D) packaging is heterogeneous stacks containing all of the components for a complete system or subsystem. The stack is constructed using known good die (KGD), and provides a high level of integration and interconnectivity. This packaging technology lends itself to flash memory and DRAM mass storage, as well as complete computers and other complex systems.
Transforming Growth Factor β1 Rescues Serum Deprivation-induced Apoptosis via the Mitogen-activated Protein Kinase (MAPK) Pathway in Macrophages (1999)

Cell death and cell survival are central components of normal development and pathologic states. Transforming growth factor beta1 (TGF-beta1) is a pleiotropic cytokine that regulates both cell growth and cell death. To better understand the molecular mechanisms that control cell death or survival, we investigated the role of TGF-beta1 in the apoptotic process by dominant-negative inhibition of both TGF-beta1 and mitogen-activated protein kinase (MAPK) signaling pathways. Murine macrophages (RAW 264.7) undergo apoptosis following serum deprivation, as determined by DNA laddering assay. However, apoptosis is prevented in serum-deprived macrophages by the presence of exogenous TGF-beta1. Using stably transfected RAW 264.7 cells with the kinase-deleted dominant-negative mutant of TbetaR-II (TbetaR-IIM) cDNA, we demonstrate that this protective effect by TGF-beta1 is completely abrogated. To determine the downstream signaling pathways, we examined TGF-beta1 effects on the MAPK pathway. We show that TGF-beta1 induces the extracellular signal-regulated kinase (ERK) activity in a time-dependent manner up to 4 h after stimulation. Furthermore, TGF-beta1 does not rescue serum deprivation-induced apoptosis in RAW 264.7 cells transfected with a dominant-negative mutant MAPK (ERK2) cDNA or in wild type RAW 264.7 cells in the presence of the MAPK kinase (MEK1) inhibitor. Taken together, our data demonstrate for the first time that TGF-beta1 is an inhibitor of apoptosis in cultured macrophages and may serve as a cell survival factor via TbetaR-II-mediated signaling and downstream intracellular MAPK signaling pathway.
Itraconazole Formulation Studies of the Melt-Extrusion Process with Mixture Design (2003)

Itraconazole is a poorly water soluble compound. One method to increase the aqueous solubility of itraconazole is through formation of a solid dispersion. The purpose of this study is to develop a 40% w/w itraconazole formulation through solid dispersion formation, using hydroxypropyl-beta-cyclodextrin (HP-beta-CD) and hydroxypropylmethyl-cellulose (HPMC) as mixture components. The solid dispersion was obtained by melt-extrusion using a twin-screw corotating melt extruder. A D-optimal mixture design was applied for the development of the optimal itraconazole formulation. The itraconazole fraction varied between 20% w/w and 50% w/w in the mixture design and the HPMC and HP-beta-CD fractions varied between 10% w/w and 60% w/w. The itraconazole formulation was optimized by producing clear extrudates, minimizing the torque, and maximizing the glass transition temperature and the apparent itraconazole solubility in 0.1 N HCl. Regression models were developed for the torque, glass transition temperature, and apparent solubility of itraconazole. High itraconazole fraction in the mixture promoted a better melt processing (minimizes torque). High HPMC fraction (>33% w/w) resulted in clear extrudates, indicating a solid dispersion and resulted in high glass transition temperature of the melt. High HP-beta-CD fraction resulted in increased apparent itraconazole solubility in 0.1 N HCl. The optimal itraconazole formulation consisted of 45% w/w HPMC and 15% HP-beta-CD w/w.
Scintigraphic Imaging of HSVtk Gene Therapy (2002)

The evolution of molecular biology has enabled the exploration of novel sophisticated gene-directed treating modalities for cancer. Suicide gene therapy - i.e. transfection of a so-called suicide gene that sensitizes target cells towards a prodrug - may offer an attractive approach to treat malignant tumors. For the development of effective clinical suicide gene therapy protocols, a non-invasive method to assay the extent, the kinetics and the spatial distribution of transgene expression is essential. This would allow investigators and physicians to assess the efficiency of experimental and therapeutic gene transfection protocols and would enable early prognosis of therapy outcome. Radionuclide imaging techniques like single photon emission computed tomography (SPECT) and positron emission tomography (PET), which can non-invasively visualize and quantify metabolic processes in vivo, are being evaluated for repetitive monitoring of transgene expression in living animals and humans. Transgene expression can be monitored directly by imaging the expression of the therapeutic gene itself, or indirectly using a reporter gene that is coupled to the therapeutic gene. Various radiopharmaceuticals have been developed and are now being evaluated for imaging of transgene expression. This review surveys the progress that has been made in the field of non-invasive nuclear imaging of transgene expression and focuses on the herpes simplex virus type 1 thymidine kinase (HSVtk) gene therapy approaches.
Spinocerebellar ataxia in a large kindred (1984)

We studied a large kindred with autosomal dominant spinocerebellar ataxia (SCA) to assess reproductive performance, the impact of genetic counseling, and linkage relationships of the SCA locus. Reproduction was not lower in those with SCA than in unaffected sibs or first cousins. Genetic counseling reduced reproduction during the risk period for development of SCA. Given autosomal dominant transmission of a single gene, we found strong evidence that the locus for SCA in this kindred is linked to the HLA loci.
Parallel expression ofSox9 andCol2a1 in cells undergoing chondrogenesis (1997)

To assess the role of the transcription factor Sox9 in cartilage formation we have compared the expression pattern of Sox9 and Col2a1 at various stages of mouse embryonic development. Expression of Col2a1 colocalized with expression of Sox9 in all chondroprogenitor cells. In the sclerotomal compartment of somites the onset of Sox9 expression preceded that of Col2a1. A perfect correlation was also seen between high levels of Sox9 expression and high levels of Col2a1 expression in chondrocytic cells. However, no Sox9 expression was detected in hypertrophic chondrocytes; only low levels of Col2a1 RNA were found in the upper hypertrophic zone. Coexpression of Sox9 and Col2a1 was also seen in the notochord. At E11.5 Sox9 expression in the brain and spinal neural tube was more widespread than that of Col2a1 although at E14.5 Sox9 and Col2a1 transcripts were colocalized in discrete areas of the brain. Distinct differences between Sox9 and Col2a1 expression were observed in the otic vesicle at E11.5. At E8.5, expression of Sox9 but not of Col2a1 was seen in the dorsal tips of the neural folds and after neural tube closure also in presumptive crest cells emigrating from the dorsal pole of the neural tube. No Col2a1 expression was detected in gonadal ridges in which high levels of Sox9 expression were detected. Together with our previous results showing that the chondrocyte-specific enhancer element of the Col2a1 gene is a direct target for Sox9, these results suggest that Sox9 plays a major role in expression of Col2a1. The correlation between high expression levels of Sox9 and high expression levels of Col2a1 in chondrocytes suggests the hypothesis that high levels of Sox9 are needed for full expression of the chondrocyte phenotype; lower levels of Sox9 such as in neuronal tissues which are also associated with lower expression levels of Col2a1 would be compatible with other cell specifications.
Silver(I)-Promoted <i>ipso</i>-Nitration of Carboxylic Acids by Nitronium Tetrafluoroborate (2015)

A novel and efficient method for the regioselective nitration of a series of aliphatic and aromatic carboxylic acids to their corresponding nitro compounds using nitronium tetrafluoroborate and silver carbonate in dimethylacetamide has been described. This transformation is believed to proceed via the alkyl-silver or aryl-silver intermediate, which subsequently reacts with the nitronium ion to form nitro substances. Mild reaction conditions, tolerant of a broad range of functional groups, and formation of only the ipso-nitrated products are the key features of this methodology when compared to known methods for syntheses of nitroalkyls and nitroarenes.
Insulin-like growth factor 1 receptors in human breast cancer and their relation to estradiol and progesterone receptors. (1988)

Insulin-like growth factor 1 (IGF1) binding sites were characterized in breast cancer. We demonstrate the presence of one high affinity binding site. Chemical cross-linking of 125I-IGF1 to breast cancer membranes in reducing condition and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed one band with an apparent molecular weight of 130,000. The specificity of the binding was studied. IGF2 was a good competitor whereas insulin competed with a potency lower than 1/100 that of IGF1. This IGF1 binding corresponded to the previously described type 1 IGF receptor (IGF1-R). IGF1-R was determined in 76 human breast cancer biopsies. Ninety-three % of the tumors were positive. The specific binding range was 0-16.4%; the geometric IGF1-R mean level was 3.9%. There was a relation (chi 2 test) between IGF1-R and progesterone receptor positivity rates (P = 0.002). The IGF1-R concentrations were correlated (Spearman test) with those of estradiol receptor (P = 0.0018) and progesterone receptor (P = 0.0011). A positive linear correlation existed between IGF1-R and estradiol receptor (P = 0.006) and between IGF1-R and progesterone receptor (P = 0.003). Our demonstration of the presence of IGF1-R in human breast cancer biopsies suggests that IGF1, acting either via the endocrine, paracrine, or autocrine pathways, could stimulate tumor growth.
&lt;title&gt;Nonlinear distributed feedback structures for optical sensor protection&lt;/title&gt; (2000)

We analyze the intensity-dependent optical response of passive optical limiters realized using distributed feedback structures which consist of alternating layers of materials possessing opposite Kerr nonlinearities. By elaborating an analytical model and employing numerical simulations, we explore device performance with respect to key requirements for passive optical limiter deployment. We prove that the proposed limiting mechanism results in complete clamping of transmitted intensity to a sensor-safe limiting value, independent of incident intensity. We provide a direct analytical expression for this limiting intensity in terms of structural and material parameters.
Estimation of Physiological Tremor from Accelerometers for Real-Time Applications (2011)

Accurate filtering of physiological tremor is extremely important in robotics assisted surgical instruments and procedures. This paper focuses on developing single stage robust algorithms for accurate tremor filtering with accelerometers for real-time applications. Existing methods rely on estimating the tremor under the assumption that it has a single dominant frequency. Our time-frequency analysis on physiological tremor data revealed that tremor contains multiple dominant frequencies over the entire duration rather than a single dominant frequency. In this paper, the existing methods for tremor filtering are reviewed and two improved algorithms are presented. A comparative study is conducted on all the estimation methods with tremor data from microsurgeons and novice subjects under different conditions. Our results showed that the new improved algorithms performed better than the existing algorithms for tremor estimation. A procedure to separate the intended motion/drift from the tremor component is formulated.