gebhart/patrepeval huggingface dataset:
A Pantothenate Kinase from Staphylococcus aureus Refractory to Feedback Regulation by Coenzyme A (2004)

The key regulatory step in CoA biosynthesis in bacteria and mammals is pantothenate kinase (CoaA), which governs the intracellular concentration of CoA through feedback regulation by CoA and its thioesters. CoaA from Staphylococcus aureus (SaCoaA) has a distinct primary sequence that is more similar to the mammalian pantothenate kinases than the prototypical bacterial CoaA of Escherichia coli. In contrast to all known pantothenate kinases, SaCoaA activity is not feedback-regulated by CoA or CoA thioesters. Metabolic labeling of S. aureus confirms that CoA levels are not controlled by CoaA or at steps downstream from CoaA. The pantothenic acid antimetabolite N-heptylpantothenamide (N7-Pan) possesses potent antimicrobial activity against S. aureus and has multiple cellular targets. N7-Pan is a substrate for SaCoaA and is converted to the inactive butyldethia-CoA analog by the downstream pathway enzymes. The analog is also incorporated into acyl carrier protein and D-alanyl carrier protein, the prosthetic groups of which are derived from CoA. The inactivation of acyl carrier protein and the cessation of fatty acid synthesis are the most critical causes of growth inhibition by N7-Pan because the toxicity of the drug is ameliorated by supplementing the growth medium with fatty acids. The absence of feedback regulation at the pantothenate kinase step allows the accumulation of high concentrations of intracellular CoA, consistent with the physiology of S. aureus, which lacks glutathione and relies on the CoA/CoA disulfide reductase redox system for protection from oxidative damage.
Calcium/calmodulin-dependent protein kinase mediates a pathway for transcriptional regulation. (1991)

Calcium influx in response to extracellular signals can modulate gene transcription. A constitutive, calcium/calmodulin-independent mutant of type II calcium/calmodulin-dependent protein kinase was capable of increasing the transcription rate of specific genes independently of protein kinase C activation. This increase was mediated by transferable cis-active elements capable of binding the transcription factor CAAT/enhancer binding protein. Therefore, the activation of type II calcium/calmodulin-dependent protein kinase in response to stimuli that increase intracellular calcium is proposed to represent a distinct second messenger pathway in calcium-mediated regulation of gene transcription.
Human Kallikrein 2 (hK2) and Prostate-Specific Antigen (PSA): Two Closely Related, but Distinct, Kallikreins in the Prostate (1998)

Recent studies on human kallikrein 2 (hK2) have revealed striking similarities and significant differences with the closely related kallikrein PSA. Both PSA and hK2 are primarily localized to the prostate and share close structural similarities. Although both kallikreins are produced by the same secretory epithelial cells in the prostate, hK2 is associated more with prostate tumors than PSA and is highly expressed in poorly differentiated cancer cells. The potent trypsin-like activity of hK2 contrasts with the weak chymotrypsin-like activity of PSA. The inactive precursor form of PSA, proPSA, is converted rapidly to active PSA by hK2, suggesting an important in vivo regulatory function by hK2 on PSA activity. The high homology between hK2 and PSA results in significant cross-reactivity to hK2 by polyclonal and some monoclonal antibodies to PSA. Future studies on both PSA and hK2 need to take into account this potential for cross-reactivity. Specific monoclonal antibodies to hK2 have now demonstrated that serum levels of hK2, like PSA, are correlated with prostate cancer. The production of hK2 protein in active protease form and specific monoclonal antibodies to the hK2 antigen will allow extensive future studies delineating the physiological and clinical utility of this new prostate antigen.
Models and methods: Effective use of technology-computed aided design in the industry (2000)

Modeling and simulation of semiconductor processes and devices are gaining importance in the semiconductor industry. Technology-computer aided design (TCAD) tools have the potential for rapid, inexpensive simulation experiments. But their effective use in the industry has been hampered by several limitations. This article highlights some of the problems and discusses possible approaches for the effective use of TCAD in the industry.
Multi‐sensor plant imaging: Towards the development of a stress‐catalogue (2009)

Agricultural production is limited by a wide range of abiotic (e.g. drought, waterlogging) and biotic (pests, diseases and weeds) stresses. The impact of these stresses can be minimized by appropriate management actions such as irrigation or chemical pesticide application. However, further optimization requires the ability to diagnose and quantify the different stresses at an early stage. Particularly valuable information of plant stress responses is provided by plant imaging, i.e. non-contact sensing with spatial resolving power: (i) thermal imaging, detecting changes in transpiration rate and (ii) fluorescence imaging monitoring alterations in photosynthesis and other physiological processes. These can be supplemented by conventional video imagery for study of growth. An efficient early warning system would need to discriminate between different stressors. Given the wide range of sensors, and the association of specific plant physiological responses with changes at particular wavelengths, this goal seems within reach. This is based on the organization of the individual sensor results in a matrix that identifies specific signatures for multiple stress types. In this report, we first review the diagnostic effectiveness of different individual imaging techniques and then extend this to the multi-sensor stress-identification approach.
Enabling new mobile applications with location proofs (2009)

Location is rapidly becoming the next "killer application" as location-enabled mobile handheld devices proliferate. One class of applications that has yet-to-emerge are those in which users have an incentive to lie about their location. These applications cannot rely solely on the users' devices to discover and transmit location information because users have an incentive to cheat. Instead, such applications require their users to prove their locations. Unfortunately, today's mobile users lack a mechanism to prove their current or past locations. Consequently, these applications have yet to take off despite their potential.
Design and analysis of an ultrawide-band distributed CMOS mixer (2005)

This paper presents the design and analysis of a novel distributed CMOS mixer for ultrawide-band (UWB) receivers. To achieve the UWB RF frequency range required for the UWB communications, the proposed mixer incorporates artificial inductance-capacitance (LC) delay lines in radio frequency (RF), local oscillator (LO), and intermediate frequency signal paths, and single-balanced mixer cells that are distributed along these LC circuits. Closed-form analytical model for the conversion gain of the mixer is presented. Furthermore, a comprehensive noise analysis of the proposed distributed mixer is carried out, which includes calculation of the mixer noise figure (NF) and derivation of the optimum number of stages, n, minimizing the NF. The designed mixer is capable of covering the RF and LO frequencies over a wide range of frequencies from 3.1-8.72 GHz. A two-stage distributed mixer has been fabricated in a 0.18-/spl mu/m CMOS process. Experiments show a conversion gain of more than 2.5 dB for the entire range of the frequencies. The dc power consumption is 10.4 mW.
Spread spectrum access methods for wireless communications (1995)

The authors present an overview of the characteristics of code division multiple access (CDMA) as it is currently being envisioned for use in wireless communications. There are many considerations in the design of such systems, and there are multiple designs being discussed. CDMA has been proposed for both terrestrial links and satellite links. However, there are key differences in the characteristics of the two types of links relative to the way they affect a CDMA system. The authors show how the ideas presented affect both terrestrial and satellite-based systems.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Early embryonic cleavage pattern, hatching success, and egg-lipid fatty acid composition: comparison between two cod (<i>Gadus morhua</i>) stocks (1997)

Baltic cod (Gadus morhua), from a brackish environment, were compared with oceanic (Skagerrak) cod in terms of early embryonic cleavage pattern, hatching success, and egg-lipid fatty acid composition. The influence of diet on these variables was investigated by analysing eggs from the Baltic stock, caught during three successive years and given controlled food for 24, 7, and 2 months, respectively, before the experiment commenced. Skagerrak cod eggs contained twice as much arachidonic acid (AA) in the phospholipid (PL) fraction as eggs from the Baltic cod, regardless of the length of time the Baltic cod had been kept in captivity and fed a control diet. Correlation between hatching rate and docosahexaenoic (DHA; 22:6n-3)/ eicosapentaenoic acid (EPA; 20:5n-3) ratio was significant (r 2 = 0.56) as well as hatching rate and AA content of the PL fraction (r 2 = 0.61). Symmetry during development, which was correlated to egg viability, was higher for eggs from Skagerrak than those from the Baltic stock. We suggest that AA content and DHA/EPA ratio of the PL fraction are important factors influencing egg viability and possibly also egg symmetry.\b Further, it appears that the factor(s) influencing the composition of the PL fraction is mostly related to stock rather than to diet.
Genome-wide analysis validates aberrant methylation in fragile X syndrome is specific to the FMR1locus (2013)

This study provides a comprehensive methylation profile of FXS and helps refine our understanding of the mechanisms behind FMR1 silencing.
Sensing Traffic Density Combining V2V and V2I Wireless Communications (2015)

Wireless technologies are making the development of new applications and services in vehicular environments possible since they enable mobile communication between vehicles (V2V), as well as communication between vehicles and infrastructure nodes (V2I). Usually, V2V communications are dedicated to the transmission of small messages mainly focused on improving traffic safety. Instead, V2I communications allow users to access the Internet and benefit from higher level applications. The combination of both V2V and V2I, known as V2X communications, can increase the benefits even further, thereby making intelligent transportation systems (ITS) a reality. In this paper, we introduce V2X-d, a novel architecture specially designed to estimate traffic density on the road. In particular, V2X-d exploits the combination of V2V and V2I communications. Our approach is based on the information gathered by sensors (i.e., vehicles and road side units (RSUs)) and the characteristics of the roadmap topology to accurately make an estimation of the instant vehicle density. The combination of both mechanisms improves the accuracy and coverage area of the data gathered, while increasing the robustness and fault tolerance of the overall approach, e.g., using the information offered by V2V communications to provide additional density information in areas where RSUs are scarce or malfunctioning. By using our collaborative sensing scheme, future ITS solutions will be able to establish adequate dissemination protocols or to apply more efficient traffic congestion reduction policies, since they will be aware of the instantaneous density of vehicles.
Nonclassical Roles of Cholinesterases in the Embryonic Brain and Possible Links to Alzheimer Disease (1995)

Evidence about nonclassic functions of acetyl- (AChE) and butyryl-cholinesterase (BChE) during embryonic development of vertebrate brains is compared with evidence of their expression in Alzheimer disease (AD). Before axons extend in the early neural tube, BChE expression shortly precedes the expression of AChE. BChE is associated with neuronal and glial cell proliferation, and it may also regulate AChE. AChE is suggested to guide and stabilize growing axons. Pathologically, cholinesterase expression in AD shows some resemblance to that in the embryo. These findings are inconsistent with the "cholinergic hypothesis." Rather, it is suggested that cholinesterases in AD function nonclassically as in the embryo, possibly as part of a "neoembryonic" restorative program. These views may open new strategies for pharmacology and therapy for AD.
RADIOACTIVE WASTE DISPOSAL IN THE SHIPROCK-TYPE URANIUM MILLING FLOWSHEET (1961)

The NODDI color maps could make this technique valuable in a clinical setting, providing comprehensive and accessible information in normal and pathological brain tissues in different neurological pathologies.
Purification and characterization of an F-actin-bundling 55-kilodalton protein from HeLa cells. (1985)

An F-actin-bundling protein with Mr of 55,000 has been purified from HeLa cells by a simple method using its affinity to F-actin. Briefly, muscle actin was mixed with supernatants of HeLa cell homogenates, and the resultant actin gel was precipitated by low speed centrifugation. The 55-kDa protein in the actin gel was dissociated by depolymerization of F-actin and purified sequentially by chromatography on DEAE-cellulose and hydroxylapatite. The Stokes radius and sedimentation coefficient of the 55-kDa protein were 32 A and 4.35 (S20,w), respectively. These results suggest that the 55-kDa protein is a monomeric globular protein with a native molecular weight of 57,000. The globular form of the protein was confirmed by electron microscopy of rotary shadowed specimens. The binding of the protein to actin was saturated at an approximate stoichiometry of 4 actin monomers to one 55-kDa molecule. The protein made F-actin aggregate side-by-side into bundles as has been reported for other F-actin-bundling proteins such as fimbrin (Mr = 68,000) and fascin (Mr = 58,000). The 55-kDa protein is a new actin-binding protein based on biochemical, morphological, and immunological characterization. Skeletal muscle tropomyosin inhibited the actin-bundling activity of 55-kDa protein by competitive binding to actin, suggesting that the 55-kDa protein binding site on F-actin is in the vicinity of the tropomyosin-binding site.
Immortal polymerization: The outset, development, and application (2000)

The story of the outset of the concept of immortal polymerization is presented. Immortal polymerization is the polymerization that gives polymers with a narrow molecular distribution, even in the presence of a chain transfer reaction, because of its reversibility, which leads to the revival of the polymers once dead, that is, the immortal nature of the polymers. As a result, immortal polymerization can afford polymers with a controlled molecular weight, the number of polymer molecules being more than that of the initiator. The compound that plays a leading role is metalloporphyrin, in which the metal-axial ligand bond has an unusually high reactivity. Immortal polymerization can be carried out in the ring-opening polymerizations of epoxides, episulfides, and lactones by the selection of an appropriate metalloporphyrin as the initiator and a protic compound as the chain transfer agent. Immortal polymerization is an effective method for synthesizing end-functional polymers and oligomers with narrow molecular weight distributions. © 2000 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 38: 2861–2871, 2000
An integrated operations solution for gate-to-gate airline operations (2011)

Modular systems for integrated airline operations and automated information management - on the ground, in the air and bi-directionally data linked - are challenging aspects to support the three key domains of a future air traffic environment successfully: Communication, Navigation, and Surveillance. In this paper, a conceptual implemented system and operational scenario is described to demonstrate a realistic example in the day of a Part 121 airline operator in 2018. Based on a bad weather situation and airport closure for several hours, the paper showcases the corresponding overall disruption management of a sample airline in a likely NextGen [1] / SESAR [2] environment. The paper describes how Communication, Navigation and Surveillance capabilities and functions, highly integrated between an Airline Operations Center (AOC) and future flight deck systems, could be handled and managed with fully automated and collaborative decision making tools. It is described how the corresponding systems and interfaces interact with each other, to manage the given emergency situation fast, efficient, safe and cost effective for the airline. Special focus is given on the 4D integration and context sensitive depiction of aeronautical information on a future flight deck. An integrated seamless depiction of static and time sensitive data is described, illustrating a bi-directionally data link to real time services in an AOC. A seamless and data driven composition and depiction of airport, departure, enroute and arrival information is explained. Operational and airline specific rules are illustrated to explain how digital information can and is operationally decluttered and filtered to support the pilots workflow and information demands best. In summary, the paper discusses an approach on of an automated and highly integrated AOC system for envisioned operations in 2018, combining systems and activities at an ANSP, at the Airlines OC, in coordination with ATC and by the crew on the flight deck. Key objectives described are looking at optimizing the efficiency and effectiveness of airline operations, adaptive to various technical fidelity infrastructure of ATC environments world wide, still ensuring a better situational awareness to operate safe and secure flights at a lower workload than today This is based on the fact that in the long term, the future airline will be connected to a System Wide Information Management (SWIM) as envisioned in the NextGen and SESAR aviation modernization programs [1, 2]. SWIM - realized in any specific implementation derivative - will be an exchange network key to ensure situational awareness between the AOC and the future flight deck. In the short term, the implementation of a local SWIM concept between AOC's and airline fleets will enable direct airline business benefits to be generated by assuring the provision of commonly understood quality information, delivered to the right people at the right time.
Resolution of spontaneous bleeding events but failure of pregnancy in fibrinogen-deficient mice. (1995)

To explore the role of the key coagulation factor, fibrinogen, in development, hemostasis, wound repair, and disease pathogenesis, we disrupted the fibrinogen A alpha chain gene in mice. Homozygous, A alpha chain-deficient (A alpha-/-) mice are born normal in appearance, and there is no evidence of fetal loss of these animals based on the Mendelian pattern of transmission of the mutant A alpha chain allele. All of the component chains of fibrinogen (A alpha, B beta, and gamma) are immunologically undetectable in the circulation of both neonatal and adult A alpha-/- mice, and blood samples fail to either clot or support platelet aggregation in vitro. Overt bleeding events develop shortly after birth in approximately 30% of A alpha-/- mice, most frequently in the peritoneal cavity, skin, and soft tissues around joints. Remarkably, most newborns displaying signs of bleeding ultimately control the loss of blood, clear the affected tissues, and survive the neonatal period. Juveniles and young adult A alpha-/- mice are predisposed to spontaneous fatal abdominal hemorrhage, but long-term survival is variable and highly dependent on genetic background. The periodic rupture of ovarian follicles in breeding-age A alpha-/- females does not appear to significantly diminish life expectancy relative to males; however, pregnancy uniformly results in fatal uterine bleeding around the tenth day of gestation. Microscopic analysis of spontaneous lesions found in A alpha-/- mice suggests that fibrin(ogen) plays a fundamental role in the organization of cells at sites of injury.
MHC class I and II antigen expression and interferon α treatment of human midgut carcinoid tumors (1994)

Cryosections of 28 primary and metastatic midgut carcinoid tumors from 12 patients with carcinoid syndrome were investigated immunohistochemically with antibodies that recognize human MHC class I (HLA-ABC) and class II (HLA-DR) antigens. The tumor parenchyma of all six patients treated with interferon alpha (IFN-alpha) during a mean 8.6 months (3 x 10(6) to 5 x 10(6) U three times weekly) exhibited unequivocal HLA-ABC immunoreactivity, with only minor discrepancies between primary lesions and metastases in mesenteric lymph nodes and liver. Class I staining was absent on the tumor cells of all 14 specimens from the patients without IFN therapy but was induced by culturing freshly dispersed tumor cells in vitro for 48 hours in the presence of recombinant IFN-alpha. The stroma of all neoplasms displayed class I and II immunostaining, as did usually a few CD4-expressing cells. The carcinoid specimens lacked parenchymal HLA-DR immunoreactivity, which is interesting considering suggestions on improved prognosis for bowel carcinomas lacking the class II expression. The study supports the idea that induction of MHC class I antigens could contribute to the beneficial clinical effect of IFN-alpha treatment in patients with midgut carcinoid tumors.
The human and murine influenza-specific B cell repertoires share a common idiotope. (1987)

We have dissected the human influenza-specific B cell repertoire by performing Epstein-Barr virus (EBV) limiting dilution analysis of lymphocytes obtained from donors before and after immunization with a commercially available influenza vaccine. In addition to an analysis of precursor frequency and light chain diversity, we studied sera and culture supernatants containing human anti-influenza antibodies with a panel of murine monoclonal antibodies specific for idiotopes identified on murine anti-PR8 and anti-X-31 antibodies. An idiotypic specificity present on the X-31-specific murine monoclonal PY206 has previously been shown to be shared by murine antibodies specific for PR8, X-31, and other influenza viruses. We observed little correlation among the following parameters: anti-viral titer, serum idiotope content, precursor frequency and immune status. More interestingly, there was a striking predominance of human influenza-specific antibodies that utilized lambda light chains. In addition, 12 of 26 human anti-influenza monoclonals strongly inhibited the binding of one of the murine anti-idiotopes to the labeled murine antibody, PY206. This is the same idiotope that is shared among murine antiinfluenza antibodies and all six individuals studied contained clones reactive with this anti-idiotope. Seven of these 12 idiotope-positive human antibodies gave partial cross-reactivity in a second anti-idiotypic system. These observations imply that a significant level of homology exists between the binding sites of human and murine influenza-specific antibodies and suggest that idiotypic manipulation of the human immune response to influenza virus may have important therapeutic implications.