gebhart/patrepeval huggingface dataset:
Identification of cellular target genes of the Epstein-Barr virus transactivator Zta: activation of transforming growth factor beta igh3 (TGF-beta igh3) and TGF-beta 1 (1995)

The lytic switch transactivator Zta initiates the ordered cascade of Epstein-Barr virus gene expression that culminates in virus production. Zta is a sequence-specific DNA-binding protein that transactivates early viral promotes via cis-acting sequences. Activation of some of these genes is mediated through binding to consensus AP-1 promoter elements. This observation suggests that Zta may also regulate the expression of cellular genes. While many targets of Zta have been identified in the Epstein-Barr virus genome, putative host cell targets remain largely unknown. To address this issue, a tetracycline-regulated Zta expression system was generated, and differential hybridization screening was used to isolate Zta-responsive cellular genes. The major target identified by this analysis is a gene encoding a fasciclin-like secreted factor, transforming growth factor beta igh3 (TGF-beta igh3), that was originally identified as a gene that is responsive to the potent immunosuppressor TGF-beta 1. Northern (RNA) blot analysis demonstrated that induction of Zta expression results in a 10-fold increase in TGF-beta igh3 mRNA levels. Zta was also found to increase TGF-beta 1 mRNA levels as well as the amount of active TGF-beta 1 secreted into the medium. Interestingly, alpha 1-collagen IV, which has been shown to potentiate the effects of TGF-beta 1, is also a cellular target of Zta. These results suggest that Zta could play a role in modulating the host cell environment through activating the expression of secreted factors.
Mechanical Behavior (2001)

No AccessPolymeric MaterialsJan 2001Mechanical BehaviorGottfried W. EhrensteinGottfried W. Ehrensteinhttps://doi.org/10.3139/9783446434134.006SectionsAboutPDF ToolsAdd to FavoritesDownload CitationTrack CitationsCopy LTI LinkPDF key 'share (en)' returned an object instead of string.FacebookTwitterEmailLinkedIn previous chapternext chapter FiguresReferencesRelatedDetails 2001Pages: 167-228Print ISBN: 978-3-446-21461-3eISBN: 978-3-446-43413-4 Copyright & Permissions© 2001 Carl Hanser Verlag GmbH & Co. KGPDF downloadLoading ...
Equine Herpesvirus 1 Enters Cells by Two Different Pathways, and Infection Requires the Activation of the Cellular Kinase ROCK1 (2007)

Equine herpesvirus type 1 (EHV-1), a member of the Alphaherpesviridae, displays a broad host range in vitro, allowing for detailed study of the mechanisms of productive infection, including attachment and entry, in various cell culture systems. Previously, we showed that EHV-1 infects Chinese hamster ovary (CHO-K1) cells even though these cells do not express a known alphaherpesvirus entry receptor. In this report, we show by electron microscopy and an infectious recovery assay that entry into CHO-K1 cells occurs via an endocytic or phagocytic mechanism, while entry into equine dermal (ED) or rabbit kidney (RK13) cells occurs by direct fusion at the cell surface. In both cases (endocytic/phagocytic or direct fusion), entry leads to productive infection. Using drugs that inhibit clathrin-dependent or caveola-dependent endocytosis, we showed that EHV-1 entry into CHO-K1 cells does not require clathrin or caveolae. We also show that EHV-1 infection requires the activation of cell signaling molecules. In particular, we demonstrate that activation of the serine/threonine Rho kinase ROCK1 is critical for infection. Inhibition of this kinase by drugs or overexpression of a negative regulator of ROCK1 significantly blocked EHV-1 infection. These results show that EHV-1 can enter disparate cell types by at least two distinct mechanisms and that productive infection is dependent upon the activation of ROCK1.
Toxic-Shock Syndrome (1980)

Surveillance for toxic-shock syndrome (TSS) in Wisconsin detected 38 cases with onsets from September 1975 through June 1980. Thirty-seven of the cases occurred after January 1, 1979; 37 of the patients were women, 35 cases occurred during menses; 38 patients were white; and one patient died. Cervical or vaginal cultures were obtained before antibiotic therapy in 23 patients, and 17 cultures were positive for Staphylococcus aureus. Ten patients had at least one recurrent episode during subsequent menstrual periods. The recurrence rate was lower in patients who had been treated with beta-lactamase-resistant antibiotics. Thirty-five patients were matched for age and menstruation to 105 controls: 34 of 35 cases (versus 80 of 105 controls) used tampons during every menstrual period (P < 0.01); nine of 35 cases (versus 64 of 105 controls) practiced contraception (P < 0.001). In Wisconsin the minimum incidence of TSS as defined by clinical criteria is 6.2 cases per 100,000 menstruating women per year. The rate of TSS among menstruating women younger than 30 years was 2.4 to 3.3 times the rate among those who were 30 or older.
Optical Matter: Crystallization and Binding in Intense Optical Fields (1990)

Properly fashioned electromagnetic fields coupled to microscopic dielectric objects can be used to create arrays of extended crystalline and noncrystalline structures. Organization can be achieved in two ways: In the first, dielectric matter is transported in direct response to the externally applied standing wave optical fields. In the second, the external optical fields induce interactions between dielectric objects that can also result in the creation of complex structures. In either case, these new ordered structures, whose existence depends on the presence of both light and polarizable matter, are referred to as optical matter.
The Economic Viability of Multilateral Wells (2000)

Abstract Multilateral wells are receiving much attention in the oil industry. Despite the increasing amount of publicity, multilateral wells in practice account for only a small fraction of the total number of wells drilled in the world today. One reason for this limited application may be that the economic benefit of drilling these wells may be in question, even though the higher construction costs associated with drilling a multilateral well can usually be justified on paper by the resulting, projected rise in well producibility. Several factors can contribute to the economic success of a multilateral project: Reservoir Charecteristics Production and Facilities Capabilities Operator and Service Company experience with multilateral installations. Technological advances and refinements in the multilateral drilling and completion system Complexity of the drilling program Effective contingency planning prior to the commencement of the drilling operations. This paper examines multilateral wells that have been drillled in the North Sea and reviews their true economic impact. The paper proposes a balanced score card for tracking and benchmarking the economic performance of these wells. The paper also discusses suggestions for enhancing the probablility of both the economic and technical succes of multilateral wells.
In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation (2007)

Thymic stromal lymphopoietin (TSLP) potently induces deregulation of Th2 responses, a hallmark feature of allergic inflammatory diseases such as asthma, atopic dermatitis, and allergic rhinitis. However, direct downstream in vivo mediators in the TSLP-induced atopic immune cascade have not been identified. In our current study, we have shown that OX40 ligand (OX40L) is a critical in vivo mediator of TSLP-mediated Th2 responses. Treating mice with OX40L-blocking antibodies substantially inhibited immune responses induced by TSLP in the lung and skin, including Th2 inflammatory cell infiltration, cytokine secretion, and IgE production. OX40L-blocking antibodies also inhibited antigen-driven Th2 inflammation in mouse and nonhuman primate models of asthma. This treatment resulted in both blockade of the OX40-OX40L receptor-ligand interaction and depletion of OX40L-positive cells. The use of a blocking, OX40L-specific mAb thus presents a promising strategy for the treatment of allergic diseases associated with pathologic Th2 immune responses.
Hybrid image/model-based gaze-contingent rendering (2009)

A nonisotropic hybrid image/model-based gaze-contingent rendering technique utilizing ray casting on a GPU is discussed. Empirical evidence derived from human subject experiments indicates an inverse relationship between a peripherally degraded scene's high-resolution inset size and mean search time, a trend consistent with existing image-based and model-based techniques. In addition, the data suggest that maintaining a target's silhouette edges decreases search times when compared to targets with degraded edges. However, analysis suggests a point of diminishing returns with an inset larger than 15° when target discrimination is a component of visual search. Benefits of the hybrid technique include simplicity of design and parallelizability, both conducive to GPU implementation.
Prognostic significance of activated CD8(+) T cell infiltrations within esophageal carcinomas. (2001)

The purpose of this study was to explore whether there is a linkage between the infiltration of CD8(+) T cells and the risk of death from esophageal cancer. Cases of 70 consecutive patients in whom esophageal squamous cell carcinomas ( n = 33) or adenocarcinomas (n = 37) were R0-resected between 1993 and 1999 were reviewed. The presence of activated CD8(+) T cells was evaluated by quantitative real-time PCR and immunohistochemistry and compared to clinical and pathological stages. The primary end point analyzed was overall survival, and a multivariate analysis was performed to distinguish any factors conferring an improved survivorship. Intratumoral (i.t.) CD8(+) T cells accumulating within the epithelial complexes were detected in 11 of all (16%) cases: in 9 of 25 (36%) patients with Union Internationale Contrele Cancer stage I or II versus 2 of 45 (4%) patients with Union Internationale Contrele Cancer stage III or IV (P = 0.001). Intratumoral CD8(+) T cell infiltrations showed proliferative activity and also IFN-gamma secretion. The presence of i.t. CD8(+) T cell infiltration more than peritumoral infiltration was associated with a good prognosis in both squamous cell and adenocarcinomas. Multivariate analysis showed that i.t. CD8(+) T cell infiltration was an independent prognostic factor (hazard ratio, 0.5; P = 0.0004) indicating favorable outcome. In conclusion, the presence of CD8(+) T cell infiltration in esophageal carcinomas is a favorable prognostic factor that should have diagnostic and therapeutic implications.
Muc-5/5ac Mucin Messenger RNA and Protein Expression Is a Marker of Goblet Cell Metaplasia in Murine Airways (2000)

Airway inflammation, hyperreactivity, increased number of goblet cells, and mucus overproduction characterize asthma. Respiratory challenge with ovalbumin (OVA) of sensitized mice has been shown by several laboratories to cause pulmonary pathology similar to that observed in human allergic asthma. Recently, interleukin (IL)-13 has been shown to be a central mediator in this process. Because the airways of healthy mice have few, if any, mucus-producing cells, an increase in the number of these cells likely reflects induction of mucin-gene expression. The purpose of this study was to identify mucin genes induced as a result of airway goblet-cell metaplasia (GCM) in mice sensitized and challenged with OVA or in mice treated with IL-13 alone. BALB/c mice were sensitized by intraperitoneal injection (Days 0, 4, 7, 11, and 14) and intranasal instillation (Day 14) of 100 microg of OVA in saline, and then challenged by intranasal instillation (Days 25, 26, and 27) of the same. IL-13-treated mice received 5 microg of IL-13 by intranasal instillation on three consecutive days. Control mice were given saline alone. All mice were studied 24 h after the last challenge. Histologic analysis of the lungs revealed both a striking peribronchial and perivascular lymphocytic and eosinophilic inflammation and airway GCM in OVA-treated mice, and also airway GCM without inflammation in IL-13-treated mice. Northern blot analysis of lung RNA demonstrated (1) expression of Muc-5/5ac messenger RNA (mRNA) in OVA-treated and IL-13-treated mice, but not in control mice; (2) expression of Muc-1 mRNA at comparable levels in all mice regardless of treatment; and (3) no expression of Muc-2 or Muc-3 mRNA in control or treated mice. Western blot analysis demonstrated the expression of Muc-5/5ac protein (both apomucin and glycosylated mucin) in lung lysates of OVA-treated (but not control) mice, and also the expression of Muc-5/5ac mucins in the bronchoalveolar lavage fluid of OVA-treated and IL-13-treated mice. These findings demonstrate that airway GCM is associated with the induction of pulmonary expression of Muc-5/5ac mRNA and mucin in murine models of allergic asthma.
Using the Web instead of a window system (1996)

We show how to deliver a sophisticated, yet intuitive, interactive application over the web using off-the-shelf web browsers as the interaction medium. This attracts a large user community, improves the rate of user acceptance, and avoids many of the pitfalls of software distribution.
WLC31-6: Performance Analysis of Multiuser MIMO CDMA Systems in Fast-Fading Channels (2006)

The performance of space-time transmit diversity is examined in a multiuser direct-sequence code division multiple access (DS-CDMA) system over fast-fading channels. The underlying space-time system employs two transmit and M receive antennas at the mobile user and receiver base station, respectively. We consider the performance of the space-time multiuser system when using the linear decorrelator detectorto combat the effect of multiuser interference. In our analysis, we derive a closed form expression for the probability of bit error in fast-fading channels. These theoretical results are then compared to simulations where it is shown that they are in excellent agreement. Our results demonstrate that, regardless of the system load, the full diversity order of 2NM for the fast- fading channel is always maintained and only a signal-to-noise ratio (SNR) loss is incurred.
A toolkit for automating and visualizing VLAN configuration (2009)

Virtual Local Area Networks (VLANs) are extensively used in enterprise networks. However, their configuration remains an ad-hoc, complex and error-prone process today. We believe that to eliminate these difficulties, there is need for automation tools, and also need for visualization tools. In this paper, we report on our experience building a VLAN management toolkit, which automates and visualizes common VLAN configuration tasks. We begin by describing common misconfigurations, and their impact on network performance and security. We next present a set of algorithms that automate the VLAN configuration tasks. These algorithms form the back end of the toolkit. The front end of the toolkit consists of an interactive graphical user interface which provides visualization of VLAN operations at multiple granularities, and can be accessed remotely from a web browser. We are in the process of deploying the toolkit at a large campus network which has thousands of switches, and around 800 VLANs. Our initial operational experience shows that the toolkit is effective in both automating configuration tasks, and identifying common misconfigurations. In particular, we have found that (i) more than 40% of the VLANs in the network have redundant links that may lead to security and performance issues. (ii) more than 30% of the VLANs in the network have missing links which may result in connectivity issues and (iii) the root-bridge placements of more than 30% of the VLANs are not optimum, which again may result in performance issues. We believe these insights highlight the benefit and importance of such a toolkit.
Coactivation of Janus Tyrosine Kinase (Jak)1 Positively Modulates Prolactin-Jak2 Signaling in Breast Cancer: Recruitment of ERK and Signal Transducer and Activator of Transcription (Stat)3 and Enhancement of Akt and Stat5a/b Pathways (2007)

Prolactin (PRL) receptors (PRLRs) have been considered selective activators of Janus tyrosine kinase (Jak)2 but not Jak1, Jak3, or Tyk2. We now report marked PRL-induced tyrosine phosphorylation of Jak1, in addition to Jak2, in a series of human breast cancer cell lines, including T47D, MCF7, and SKBR3. In contrast, PRL did not activate Jak1 in immortalized, noncancerous breast epithelial lines HC11, MCF10A, ME16C, and HBL-100, or in CWR22Rv1 prostate cancer cells or MDA-MB-231 breast cancer cells. However, introduction of exogenous PRLR into MCF10A, ME16C, or MDA-MB-231 cells reconstituted both PRL-Jak1 and PRL-Jak2 signals. In vitro kinase assays verified that PRL stimulated enzymatic activity of Jak1 in T47D cells, and PRL activated Jak1 and Jak2 with indistinguishable time and dose kinetics. Relative Jak2 deficiency did not cause PRLR activation of Jak1, because overexpression of Jak2 did not interfere with PRL activation of Jak1. Instead, PRL activated Jak1 through a Jak2-dependent mechanism, based on disruption of PRL activation of Jak1 after Jak2 suppression by 1) lentiviral delivery of Jak2 short hairpin RNA, 2) adenoviral delivery of dominant-negative Jak2, and 3) AG490 pharmacological inhibition. Finally, suppression of Jak1 by lentiviral delivery of Jak1 short hairpin RNA blocked PRL activation of ERK and signal transducer and activator of transcription (Stat)3 and suppressed PRL activation of Jak2, Stat5a, Stat5b, and Akt, as well as tyrosine phosphorylation of PRLR. The data suggest that PRL activation of Jak1 represents a novel, Jak2-dependent mechanism that may serve as a regulatory switch leading to PRL activation of ERK and Stat3 pathways, while also serving to enhance PRL-induced Stat5a/b and Akt signaling.
Immunization with Modified Vaccinia Virus Ankara-Based Recombinant Vaccine against Severe Acute Respiratory Syndrome Is Associated with Enhanced Hepatitis in Ferrets (2004)

Severe acute respiratory syndrome (SARS) caused by a newly identified coronavirus (SARS-CoV) is a serious emerging human infectious disease. In this report, we immunized ferrets (Mustela putorius furo) with recombinant modified vaccinia virus Ankara (rMVA) expressing the SARS-CoV spike (S) protein. Immunized ferrets developed a more rapid and vigorous neutralizing antibody response than control animals after challenge with SARS-CoV; however, they also exhibited strong inflammatory responses in liver tissue. Inflammation in control animals exposed to SARS-CoV was relatively mild. Thus, our data suggest that vaccination with rMVA expressing SARS-CoV S protein is associated with enhanced hepatitis.
Residual Dense Network for Image Super-Resolution (2018)

A very deep convolutional neural network (CNN) has recently achieved great success for image super-resolution (SR) and offered hierarchical features as well. However, most deep CNN based SR models do not make full use of the hierarchical features from the original low-resolution (LR) images, thereby achieving relatively-low performance. In this paper, we propose a novel residual dense network (RDN) to address this problem in image SR. We fully exploit the hierarchical features from all the convolutional layers. Specifically, we propose residual dense block (RDB) to extract abundant local features via dense connected convolutional layers. RDB further allows direct connections from the state of preceding RDB to all the layers of current RDB, leading to a contiguous memory (CM) mechanism. Local feature fusion in RDB is then used to adaptively learn more effective features from preceding and current local features and stabilizes the training of wider network. After fully obtaining dense local features, we use global feature fusion to jointly and adaptively learn global hierarchical features in a holistic way. Extensive experiments on benchmark datasets with different degradation models show that our RDN achieves favorable performance against state-of-the-art methods.
A cyclic‐di‐GMP receptor required for bacterial exopolysaccharide production (2007)

Bis-(3',5')-cyclic-dimeric-guanosine monophosphate (c-di-GMP) has been shown to be a global regulatory molecule that modulates the reciprocal responses of bacteria to activate either virulence pathways or biofilm formation. The mechanism of c-di-GMP signal transduction, including recognition of c-di-GMP and subsequent phenotypic regulation, remain largely uncharacterized. The key components of these regulatory pathways are the various adaptor proteins (c-di-GMP receptors). There is compelling evidence suggesting that, in addition to PilZ domains, there are other unidentified c-di-GMP receptors. Here we show that the PelD protein of Pseudomonas aeruginosa is a novel c-di-GMP receptor that mediates c-di-GMP regulation of PEL polysaccharide biosynthesis. Analysis of PelD orthologues identified a number of conserved residues that are required for c-di-GMP binding as well as synthesis of the PEL polysaccharide. Secondary structure similarities of PelD to the inhibitory site of diguanylate cyclase suggest that a common fold can act as a platform to bind c-di-GMP. The combination of a c-di-GMP binding site with a variety of output signalling motifs within one protein domain provides an explanation for the specificity for different cellular responses to this regulatory dinucleotide.
No effect of antiviral (valacyclovir) treatment in fibromyalgia: a double blind, randomized study. (2004)

Valacyclovir cannot be recommended as a therapy for FM at this point.
Removal of arsenic from wastewater using chemical precipitation methods (1992)

ABSTRACT: The use of arsenic in agriculture, industry, and domestic endeavors since the last century has increased the concentration of arsenic in the environment. During cleanup activities at a former pesticide facility, water that was collected during various operations was contaminated with arsenic and required treatment before discharge. Chemical precipitation was identified as being the most effective means of treatment. Bench‐scale treatability testing was completed to determine the effectiveness of various coagulants, including ferric chloride, hydrated lime, sodium sulfide, and alum. A combination of hydrated lime and ferric chloride was able to remove more than 99% of the original arsenic concentration. Contaminated water to be treated by the full‐scale system had arsenic concentrations up to 48 mg/L. The discharge limitation required that the arsenic concentration be reduced to less than 1 mg/L. More than 605 000 L (160 000 gal) of water were treated using a combination of chemical precipitation, filtration, and carbon absorption.
Maximum likelihood voice conversion based on GMM with STRAIGHT mixed excitation (2006)

INTERSPEECH2006: the 9th International Conference on Spoken Language Processing (ICSLP), September 17-21, 2006, Pittsburgh, Pennsylvania, USA.
Evaluation of 16 kbit/s digital voice transmission for mobile radio (1984)

In this paper 16 kbit/s digital voice transmission with conventional channel spacing of 25 kHz, employing a 16 kbit/s adaptive delta modulation (ADM) coder-decoder (CODEC) is evaluated. The main characteristics of narrow-band digital FM modulation schemes, such as tamed FM, Gaussian filtered minimum shift keying (GMSK), four-level FM and phase locked loop-quaternary phase shift keying (PLL-QPSK), are compared by laboratory tests. Digitized voice quality in a digital channel incorporating a 16 kbit/s ADM CODEC and GMSK coherent detection was compared with voice quality of a conventional analog FM channel. Bit error ratio (BER) performance is shown to depend primarily on demodulation schemes. Digital voice quality is inferior to that of analog voice with an opinion score difference of about 0.5 in fading environments. This kind of digital voice transmission will be applicable for those systems that require high security at an expense of speech quality.