gebhart/patrepeval huggingface dataset:
Binding of the Drosophila transformer and transformer-2 proteins to the regulatory elements of doublesex primary transcript for sex-specific RNA processing. (1992)

Sex-specific alternative processing of double-sex (dsx) precursor messenger RNA (pre-mRNA) is one of the key steps that regulates somatic sexual differentiation in Drosophila melanogaster. By transfection analyses using dsx minigene constructs, we identified six copies of the 13-nucleotide sequences TC(T/A)(T/A)C(A/G)ATCAACA in the female-specific fourth exon that act as the cis elements for the female-specific splicing of dsx pre-mRNA. UV-crosslinking experiments revealed that both female-specific transformer (tra) and transformer-2 (tra-2) products bind to the 13-nucleotide sequences of dsx pre-mRNA. These results strongly suggest that the female-specific splicing of dsx pre-mRNA is activated by binding of these proteins to the 13-nucleotide sequences.
Pharmacokinetics and metabolism of sildenafil in mouse, rat, rabbit, dog and man (1999)

1. Pharmacokinetics were studied in mouse, rat, rabbit, dog and man after single intravenous and/or oral doses of sildenafil or [14C]-sildenafil (Viagra). 2. In man, absorption from the gastrointestinal tract was essentially complete. With the exception of male rat, Tmax occurred at approximately 1 h or less. Bioavailability was attenuated by pre-systemic hepatic metabolism in all species. 3. The volume of distribution was similar in rodents and humans (1-2 l/kg) but was greater in dog (5.2 l/kg), due to lower plasma protein binding (84 versus 94-96% respectively). 4. High clearance was the principal determinant of short elimination half-lives in rodents (0.4-1.3 h), whereas moderate clearance in dog and man resulted in longer half-lives (6.1 and 3.7 h respectively). Clearances were in agreement with in vitro metabolism rates by liver microsomes from the various species. 5. After single oral or intravenous doses of [14C]-sildenafil, the majority of radioactivity was excreted in the faeces of all species. No unchanged drug was detected in the excreta of man. 6. Five principal pathways of metabolism in all species were piperazine N-demethylation, pyrazole N-demethylation, loss of a two-carbon fragment from the piperazine ring (N,N'-deethylation), oxidation of the piperazine ring and aliphatic hydroxylation. Additional metabolites arose through combinations of these pathways. 7. Sildenafil was the major component detected in human plasma. Following oral doses, AUC(infinity) for the piperazine N-desmethyl and piperazine N,N'-desethyl metabolites were 55 and 27% that of parent compound respectively.
A Specific Inhibitor of the Epidermal Growth Factor Receptor Tyrosine Kinase (1994)

A small molecule called PD 153035 inhibited the epidermal growth factor (EGF) receptor tyrosine kinase with a 5-pM inhibition constant. The inhibitor was specific for the EGF receptor tyrosine kinase and inhibited other purified tyrosine kinases only at micromolar or higher concentrations. PD 153035 rapidly suppressed autophosphorylation of the EGF receptor at low nanomolar concentrations in fibroblasts or in human epidermoid carcinoma cells and selectively blocked EGF-mediated cellular processes including mitogenesis, early gene expression, and oncogenic transformation. PD 153035 demonstrates an increase in potency over that of other tyrosine kinase inhibitors of four to five orders of magnitude for inhibition of isolated EGF receptor tyrosine kinase and three to four orders of magnitude for inhibition of cellular phosphorylation.
&lt;title&gt;Interactive 3-D graphics workstations in stereotaxy: clinical requirements, algorithms, and solutions&lt;/title&gt; (1992)

In stereotactic treatment planning the spatial relationships between a variety of objects has to be taken into account in order to avoid destruction of vital brain structures and rupture of vasculature. The visualization of these highly complex relations may be supported by 3-D computer graphics methods. In this context the three-dimensional display of the intracranial vascular tree and additional objects, such as neuroanatomy, pathology, stereotactic devices, or isodose surfaces, is of high clinical value. We report an advanced rendering method for a depth-enhanced maximum intensity projection from magnetic resonance angiography (MRA) and a walk-through approach to the analysis of MRA volume data. Furthermore, various methods for a multiple-object 3-D rendering in stereotaxy are discussed. The development of advanced applications in medical imaging can hardly be successful if image acquisition problems are disregarded. We put particular emphasis on the use of conventional MRI and MRA for stereotactic guidance. The problem of MR distortion is discussed and a novel three- dimensional approach to the quantification and correction of the distortion patterns is presented. Our results suggest that the sole use of MR for stereotactic guidance is highly practical. The true three-dimensionality of the acquired datasets opens up new perspectives to stereotactic treatment planning. For the first time it is possible now to integrate all the necessary information into 3-D scenes, thus enabling an interactive 3-D planning.
Frontiers in Intravascular Imaging Technologies (2008)

I n 1971, Bom et al 1 developed one of the first catheter- based real-time imaging techniques for use in the cardiac system. In placing a set of phased-array ultrasound transducers within the cardiac chambers, Bom and colleagues showed that higher frequencies than those used in transthoracic ultrasound imaging could be used to produce high-resolution images of cardiac structures. By the late 1980s, Yock et al 2 had successfully miniaturized a single-transducer system to enable transducer placement within coronary arteries. Since then, intravascular ultrasound (IVUS) has become a pivotal catheter-based imaging technology, having provided practical guidance for percutaneous interventions and scientific insights into vascular biology in clinical settings. Technical developments currently being explored consist of further device improvements, a variety of advanced image analyses, and the extension of this ultrasound-based approach to diverse intravascular imaging techniques with other energy sources.
Management of Female Genital Warts with an Analog of Imiquimod 2% in Cream: A Randomized, Double‐Blind, Placebo‐Controlled Study (1998)

The purpose of this randomized, double-blind, placebo-controlled study was to determine the clinical efficacy and tolerability of an analog of imiquimod (2%)in cream to cure genital warts in women. Sixty preselected women, ranging between 18 and 45 years of age (mean 24.3) and having 411 lesions (mean 6.8) with clinical, histopathological and polymerase chain reaction (PCR) confirmed diagnosis of human papilloma virus (HPV) infection were randomized to two parallel groups. Each patient received a precoded 40-g tube and instructions on how to apply the trial medication to their lesions at home two times daily for five consecutive days per week. The active treatment period was six weeks. Patients were evaluated on a weekly basis. A clinically and PCR established total clearance of target warts was recorded as a cure. By the end of the treatment, 43.3% of patients and 42.8% of warts were cured. Code disclosure revealed that imiquimod cream had cured 83.3% of the treated patients and 84.3% of the tested warts, while the placebo healed one subject and four warts (p < 0.0001). Eight patients (13.3%) in the imiquimod group experienced mild to moderate, non-objective, drug-induced symptoms with no dropouts. Among the 26 cured patients, five had a relapse after 11 months. In conclusion, the data presented demonstrate that 2% imiquimod in cream with mild to moderate subjective side effects is significantly more effective than placebo in eliminating genital warts in women.
Lycopene Interferes With Cell Cycle Progression and Insulin-Like Growth Factor I Signaling in Mammary Cancer Cells (2000)

Recent studies have shown that high insulin-like growth factor I (IGF-I) blood level is a risk factor in breast and prostate cancer. The aim of this study was to determine whether the mitogenic activity of IGF-I in mammary cancer cells can be reduced by the dietary carotenoid lycopene. The anticancer activity of lycopene, the major tomato carotenoid, has been suggested by in vitro, in vivo, and epidemiological studies. Growth stimulation of MCF7 mammary cancer cells by IGF-I was markedly reduced by physiological concentrations of lycopene. The inhibitory effects of lycopene on MCF7 cell growth were not accompanied by apoptotic or necrotic cell death, as determined by annexin V binding to plasma membrane and propidium iodide staining of nuclei in unfixed cells. Lycopene treatment markedly reduced the IGF-I stimulation of tyrosine phosphorylation of insulin receptor substrate 1 and binding capacity of the AP-1 transcription complex. These effects were not associated with changes in the number or affinity of IGF-I receptors, but with an increase in membrane-associated IGF-binding proteins, which were previously shown in different cancer cells to negatively regulate IGF-I receptor activation. The inhibitory effect of lycopene on IGF signaling was associated with suppression of IGF-stimulated cell cycle progression of serum-starved, synchronized cells. Moreover, in cells synchronized by mimosine treatment, lycopene delayed cell cycle progression after release from the mimosine block. Collectively, the above data suggest that the inhibitory effects of lycopene on MCF7 cell growth are not due to the toxicity of the carotenoid but, rather, to interference in IGF-I receptor signaling and cell cycle progression.
Combination Therapy With Sirolimus and Interleukin-2 Prevents Spontaneous and Recurrent Autoimmune Diabetes in NOD Mice (2002)

Sirolimus is an immunosuppressant that inhibits interleukin (IL)-2 signaling of T-cell proliferation but not IL-2-induced T-cell apoptosis. Therefore, we hypothesized that administration of IL-2, together with sirolimus, might shift T-cell proliferation to apoptosis and prevent autoimmune destruction of islet beta-cells. We found that sirolimus and IL-2 therapy of female NOD mice, beginning at age 10 weeks, was synergistic in preventing diabetes development, and disease prevention continued for 13 weeks after stopping sirolimus and IL-2 therapy. Similarly, sirolimus and IL-2 were synergistic in protecting syngeneic islet grafts from recurrent autoimmune destruction after transplantation in diabetic NOD mice, and diabetes did not recur after stopping sirolimus and IL-2 combination therapy. Immunocytochemical examination of islet grafts revealed significantly decreased numbers of leukocytes together with increased apoptosis of these cells in mice treated with sirolimus and IL-2, whereas beta-cells were more numerous, and significantly fewer were apoptotic. In addition, Th1-type cells (gamma-interferon-positive and IL-2(+)) were decreased the most, and Th2-type cells (IL-4(+) and IL-10(+)) and Th3-type cells (transforming growth factor-beta1(+)) were increased the most in islet grafts of sirolimus and IL-2-treated mice. We conclude that 1) combination therapy with sirolimus and IL-2 is synergistic in protecting islet beta-cells from autoimmune destruction; 2) diabetes prevention continues after withdrawal of therapy; and 3) the mechanism of protection involves a shift from Th1- to Th2- and Th3-type cytokine-producing cells, possibly due to deletion of autoreactive Th1 cells.
Photoinduced Oxidation of Methylsiloxane Monolayers Chemisorbed on TiO<sub>2</sub> (1996)

Photoinduced oxidation of chemisorbed 1,3,5,7-tetramethylcyclotetrasiloxane (TMCTS) in air at room temperature yielded a uniform SiOx monolayer with a thickness of approximately 0.2 nm on TiO2. The oxidation was suggested to be caused by the band gap excitation of TiO2 leading to the generation of activated oxygen species on the surface. The sequential cycles of chemisorption of TMCTS (process I) followed by photoirradiation (wavelength < 330 nm, process II) grew the SiOx (2 < x < 3) film 0.4 ± 0.1 nm per cycle, whereas the rate of the oxidation exponentially decreased with an increase in the film thickness. The contact angle of H2O, being almost constant at 90° ± 3° after each process I, decreased as the photoirradiation time increased during the subsequent process II. The gas phase adsorption experiments of pyridine further demonstrated that the surface acidic nature drastically changes from Lewis acid character to Bronsted acid character upon formation of the SiOx monolayer on TiO2. The photocatalytic activity was maintained to an appreciable extent in this state. Both the analyses of the diffuse reflectance infrared Fourier transform spectra and the data on the contact angle of H2O indicated that the surface oxidation obeys the Langmuir−Hinshelwood mechanism.
Highly Potent Inhibitors of Quorum Sensing in Staphylococcus aureus Revealed Through a Systematic Synthetic Study of the Group-III Autoinducing Peptide (2013)

Methods to intercept bacterial quorum sensing (QS) have attracted significant attention as potential anti-infective therapies. Staphylococcus aureus is a major human pathogen that utilizes autoinducing peptide (AIP) signals to mediate QS and thereby regulate virulence. S. aureus strains are categorized into four groups (I-IV) according to their AIP signal and cognate extracellular receptor, AgrC. Each group is associated with a certain disease profile, and S. aureus group-III strains are responsible for toxic shock syndrome and have been underestimated in other infections to date. A limited set of non-native AIP analogs have been shown to inhibit AgrC receptors; such compounds represent promising tools to study QS pathways in S. aureus . We seek to expand this set of chemical probes and report herein the first design, synthesis, and biological testing of AIP-III mimetics. A set of non-native peptides was identified that can inhibit all four of the AgrC receptors (I-IV) with picomolar IC50 values in reporter strains. These analogs also blocked hemolysis by wild-type S. aureus group I-IV strains-a virulence trait under the control of QS-at picomolar concentrations. Moreover, four of the lead AgrC inhibitors were capable of attenuating the production of toxic shock syndrome toxin-1 (also under the control of QS) by over 80% at nanomolar concentrations in a wild-type S. aureus group-III strain. These peptides represent, to our knowledge, the most potent synthetic inhibitors of QS in S. aureus known, and constitute new and readily accessible chemical tools for the study of the AgrC system and virulence in this deadly pathogen.
Accounting for temporal evolutions in highly reactive decision-making (2002)

Applications such as aircraft combat simulation require both dynamic supervision and real-time decision-making: actions and observations interact in a reactive way, and durations between two events must be accounted for. We start from a decision-tree model, which owns strong context handling capabilities, but provides only static decision-making. We improve it by matching possible temporal chronicles, accounting for dynamic evolutions, to the context. A decision in this framework is activated upon complete recognition of such a chronicle, whereas our highly reactive application domain requires anticipated decisions, comparing possible evolutions to take the best decision in real-time. We choose to dynamically compute a timed game automaton synthesizing the predicted possible next steps, and propose an algorithm that computes the best decision from this simple controller model.
Independent Association of Lobular Involution and Mammographic Breast Density With Breast Cancer Risk (2010)

Lobular involution and MBD are independently associated with breast cancer incidence; combined, they are associated with an even greater risk for breast cancer.
Humidity sensing properties of the sensor based on graphene oxide films with different dispersion concentrations (2011)

This paper presents the humidity sensing properties of the sensor based on graphene oxide (GO) films with different dispersion concentrations. A CMOS interdigital capacitance was used as the basic structure. The relationship between the sensing properties and dispersion concentrations is studied, by dripping four GO dispersions of different concentrations on the sensing area of the sensor chip, which was then baked for 2 hours at 50 °C. The measured results imply that the sensor based on GO films with proper concentration shows monotonic relationship between the sensing capacitance and relative humidity, and is of fast response and good repeatability.
New advances and developments in the Stepanov method for the growth of shaped crystals (2002)

The main recently developed versions of the Stepanov method are analyzed, and a brief review of the main achievements in the growth of shaped crystals is given.
Accelerating Conservative Parallel Simulation of VHDL Circuits (1994)

Abstract : This research effort considers heuristic and cost model based techniques for the optimal partitioning of VHDL circuits for parallel simulation. Correlation statistics are gathered on a wide variety of graph-based a priori parameters. Linear regression is used to identify significant parameters for inclusion in a representative cost model. Driving a greedy search, this cost model is used to improve upon initial heuristic partitions. The influence of feedback dominated previous research so a no-feedback algorithm is used to create the initial partition The circuits studied range from 1,050 to 4,243 gates.
Multi-Threshold Asynchronous Circuit Design for Ultra-Low Power (2008)

This paper presents an ultra-low power circuit design methodology which combines the Multi-Threshold CMOS (MTCMOS) technique with quasi delay-insensitive (QDI) asynchronous logic, in order to solve the three major problems of synchronous MTCMOS circuits: (1) Sleep signal generation, (2) storage element data loss during sleep mode, and (3) sleep transistor sizing. In contrast to most power reduction methods that result in area overhead, the QDI asynchronous MTCMOS circuits are usually smaller than their original versions. Moreover, QDI circuits utilize handshaking protocols instead of clocks for circuit control, resulting in flexible timing requirements, which yields increased circuit robustness and allows for extreme supply voltage scaling to subthreshold region for further power reduction, without requiring any circuit modifications. This QDI asynchronous MTCMOS methodology is used to design a 4-stage pipelined 8-bit x 8-bit unsigned multiplier, which is then compared against the original QDI design (i.e., without incorporating MTCMOS) and its synchronous version. All designs use the IBM 8RF-DM 0.13 μm process. Results show 150x and 1.8x leakage power and active energy reductions on average in the QDI asynchronous MTCMOS design compared to the original QDI version, respectively.
Nodulating ability of <i>Rhizobium tropici</i> is conditioned by a plasmid‐encoded citrate synthase (1994)

Rhizobium species elicit the formation of nitrogen-fixing root nodules through a complex interaction between bacteria and plants. Various bacterial genes involved in the nodulation and nitrogen-fixation processes have been described and most have been localized on the symbiotic plasmids (pSym). We have found a gene encoding citrate synthase on the pSym plasmid of Rhizobium tropici, a species that forms nitrogen-fixing nodules on the roots of beans (Phaseolus vulgaris) and trees (Leucaena spp.). Citrate synthase is a key metabolic enzyme that incorporates carbon into the tricarboxylic acid cycle by catalysing the condensation of acetyl-CoA and oxaloacetic acid to form citrate. R. tropici pcsA (the plasmid citrate synthase gene) is closely related to the corresponding genes of Proteobacteria. pcsA inactivation by a Tn5-mob insertion causes the bacteria to form fewer nodules (30-50% of the original strain) and to have a decreased citrate synthase activity in minimal medium with sucrose. A clone carrying the pcsA gene complemented all the phenotypic alterations of the pcsA mutant, and conferred Rhizobium leguminosarum bv. phaseoli (which naturally lacks a plasmid citrate synthase gene) a higher nodulation and growth capacity in correlation with a higher citrate synthase activity. We have also found that pcsA gene expression is sensitive to iron availability, suggesting a possible role of pcsA in iron uptake.
Gene Therapy with B7.1 and GM-CSF Vaccines in a Murine AML Model (1997)

These results suggest that tumor burden and vaccine efficiency are most likely to be the limiting factors in the curative potential of tumor vaccines. Novel approaches such as this experiment could provide improved therapeutic outcomes in patients with AML and other cancers.
Optically active uniform potassium and lithium rare earth fluoride nanocrystals derived from metal trifluroacetate precursors (2009)

This paper reports the first systematical synthesis of near-monodisperse potassium and lithium rare earth (RE) fluoride (K(Li)REF4) nanocrystals with diverse shapes (cubic KLaF4 and KCeF4 wormlike nanowires, nanocubes and nanopolyhedra; cubic LiREF4 (RE = Pr to Gd, Y) nanopolyhedra; tetragonal LiREF4 (RE = Tb to Lu, Y) rhombic nanoplates) via co-thermolysis of Li(CF3COO) or K(CF3COO) and RE(CF3COO)3 in a hot oleic acid/oleylamine/1-octadecene solution. The effects of the solvent composition, reaction temperature and time on the crystal phase purity, shape, and size of the as-prepared nanocrystals have been investigated in detail. The formation of monodisperse nanocrystals is found to strongly depend upon the nature of both alkali metals from Li to K, and the rare earth series from La to Lu and Y. Based on the series of experimental results, a controlled-growth mechanism has also been proposed. In addition, the ease of doping of these as-synthesized host nanocrystals for designed luminescence properties is assessed. For example, monodisperse and single-crystalline Eu3+ doped KGdF4, Yb3+ and Er3+ co-doped LiYF4 nanocrystals redispersed in cyclohexane exhibit visible room-temperature red and green emissions under ultraviolet (UV) excitation and near infrared (NIR) 980 nm laser excitation, respectively.
The Eccentric Spheres Model as the Basis for a Study of the Role of Geometry and Inhomogeneities in Electrocardiography (1979)

To study the role of internal geometry and inhomogeneities in the "forward problem" of electrocardiography (ECG), a mathematical model was constructed which permitted manipulation of these variables. The model, which consists of two eccentric systems of concentric spheres, contains all the important torso compartments, namely the blood cavity, the myocardium, the pericardium, the lung region, the surface muscle layer, and the subcutaneous fat. An analytic solution is found in the form of a double series expansion in Legendre polynomials. The integrated effect of the inhomogeneities on the surface potential distribution is investigated. The model demonstrates the importance of interactions between the various torso components in determining the potential distribution at the surface.
High quality diesel via the Fischer–Tropsch process – a review (2001)

Abstract The Fischer–Tropsch (FT) synthesis is used to produce chemicals, gasoline and diesel fuel. The FT products are predominantly linear, hence the quality of the diesel fuel is very high, having cetane numbers of up to 75. Since purified synthesis gas is used in the FT process all the products are S‐ and N‐free. In this review the production of syngas and the various options used in the FT process (reactors and catalyst types, and high and low temperature operation) are discussed. The best FT option for producing high quality diesel is using cobalt‐based catalyst in slurry phase reactor, gearing the process for high wax production and then selectively hydrocracking the wax to diesel fuel. The overall diesel pool has a high cetane number, the aromatic S and N contents are zero and the exhaust emissions are significantly lower than for standard diesel fuels. © 2001 Society of Chemical Industry