gebhart/patrepeval huggingface dataset:
2,2-Bis(ethoxycarbonyl)- and 2-(Alkylaminocarbonyl)-2-cyano-Substituted 3-(Pivaloyloxy)propyl Groups as Biodegradable Phosphate Protections of Oligonucleotides (2005)

Oligonucleotides bearing biodegradable phosphate protecting groups have been synthesized on a solid support. For this purpose, two dimeric building blocks, viz. 5'-O-(4,4'-dimethoxytrityl)-(R(P),S(P))-O(P)-[2,2-bis(ethoxycarbonyl)-3-(pivaloyloxy)propyl]-P-thiothymidylyl-(3',5')-thymidine 3'-[O-(2-cyanoethyl)-N,N-diisopropylphosphoramidite] (1) and 5'-O-(4,4'-dimethoxytrityl)-(R(P),S(P))-O(P)-[2-cyano-2-(2-phenylethylaminocarbonyl)-3-(pivaloyloxy)propyl]thymidylyl-(3',5')-thymidine 3'-(H-phosphonate) (2), were prepared. Phosphoramidite 1 was incorporated into an phosphorothioate oligothymidylate sequence on a base-labile hydroquinone-O,O'-diacetic acid linker (Q-linker) and on a photolabile 4-alkoxy-5-methoxy-2-nitrobenzyl carbonate linker (11). H-Phosphonate 2 was, in turn, incorporated into an oligothymidylate sequence only on the photolabile linker. Kinetics of the removal of the protecting groups by porcine liver esterase and subsequent retro aldol condensation/phosphate elimination were then studied. While the pro-oligonucleotide that contained only one phosphate protection gave the deprotected phosphorothioate oligonucleotide in a quantitative yield, the enzymatic step was markedly decelerated upon increasing the number of protection groups, and hence chain cleavage started to compete.
Single-chip multiple-frequency filters based on contour-mode aluminum nitride piezoelectric micromechanical resonators (2005)

This paper reports experimental results on a new class of single-chip multiple-frequency (up to 236 MHz) filters that are based on low motional resistance contour-mode aluminum nitride piezoelectric micromechanical resonators. For the first time, aluminum nitride rectangular plates and rings have been electrically cascaded to yield high performance, low insertion loss (as low as 4dB at 93 MHz) micromechanical band pass filters. This novel technology could revolutionize wireless communication systems by allowing the co-fabrication of multiple frequency filters (IF and RF) on the same chip, therefore reducing form factors and manufacturing costs. In addition, these filters require terminations on the order of k/spl Omega/, thereby making possible their direct interface with standard 50 /spl Omega/ systems.
Improving performance of large physically indexed caches by decoupling memory addresses from cache addresses (2001)

Modern CPUs often use large physically indexed caches that are direct-mapped or have low associativities. Such caches do not interact well with virtual memory systems. An improperly placed physical page will end up in a wrong place in the cache, causing excessive conflicts with other cached pages. Page coloring has been proposed to reduce the conflict misses by carefully placing pages in the physical memory. While page coloring works well for some applications, many factors limit its performance. Page coloring limits the freedom of the page placement system and may increase swapping traffic. In this paper, we propose a novel and simple architecture, called color-indexed, physically tagged caches, which can significantly reduce the conflict misses. With some simple modifications to the TLB (translation look-aside buffer), the new architecture decouples the addresses of the cache from the addresses of the main memory. Since the cache addresses do not depend on the the physical memory addresses anymore, the system can freely place data in any cache page to minimize the conflict misses, without affecting the paging system. Extensive trace-driven simulation results show that our design performs much better than traditional page coloring techniques. The new scheme enables a direct-mapped cache to achieve hit ratios very close to or better than those of a two-way set associative cache. Moreover, the architecture does not increase cache access latency, which is a drawback of set associative caches. The hardware overhead is minimal. We show that our scheme can reduce the cache size by 50 percent without sacrificing performance. A two-way set associative cache that uses this strategy can perform very close to a fully associative cache.
Structure of limonene synthase, a simple model for terpenoid cyclase catalysis (2007)

The crystal structure of (4S)-limonene synthase from Mentha spic ata, a metal ion-dependent monoterpene cyclase that catalyzes the coupled isomerization and cyclization of geranyl diphosphate, is reported at 2.7-A; resolution in two forms liganded to the substrate and intermediate analogs, 2-fluorogeranyl diphosphate and 2-fluorolinalyl diphosphate, respectively. The implications of these findings are described for domain interactions in the homodimer and for changes in diphosphate-metal ion coordination and substrate binding conformation in the course of the multistep reaction.
Immunoglobulin lambda light-chain-related genes 14.1 and 16.1 are expressed in pre-B cells and may encode the human immunoglobulin omega light-chain protein. (1989)

Human pre-B cells, which produce immunoglobulin heavy chain but do not produce immunoglobulin light chain, are shown to contain a 1-kilobase transcript homologous to immunoglobulin lambda light-chain genes. Detailed analysis of RNA and cDNA clones derived from these transcripts reveals that they originate from the distinct immunoglobulin lambda-like genes 14.1/16.1. Sequence analysis of these clones reveals a long open reading frame, beginning with an ATG, capable of encoding a protein of 214 amino acids with an unprocessed molecular weight of 22,944. The C-terminal half of this predicted protein is highly homologous to immunoglobulin lambda light-chain joining and constant region protein sequence, while the amino-terminal end does not share homology with variable regions. Unlike immunoglobulin genes, these genes do not undergo rearrangement prior to expression. Analysis of a panel of 26 hematopoietic cell lines reveals that expression of 14.1/16.1 is limited to pre-B cells and one B-cell line, which, like the pre-B cells, is surface immunoglobulin negative. Antisera raised against a peptide whose sequence was predicted from the 14.1 cDNA sequence identifies a 22-kDa protein in human pre-B cells. Immunoprecipitation of immunoglobulin mu-chain from these pre-B cells with anti-immunoglobulin mu antibody coprecipitates a 22-kDa protein, which is a candidate for the human immunoglobulin omega light-chain protein and may be the protein product of the 14.1/16.1 genes.
A novel wideband coupled-line Gysel power divider with function of impedance matching (2012)

A new coupled-line circuit structure is proposed to design compact wideband Gysel power dividers with function of impedance matching between source and load. Based on traditional even- and odd-mode technique, closed-form equations are obtained for direct and accurate synthesis. By applying coupled lines and reducing their electrical lengths, this novel Gysel power divider has four main advantages: high power-handling capability, small size, enhanced bandwidth, and source-to-load impedance matching. The measured bandwidth ( & dB) of the fabricated Gysel power divider operating at 2 GHz is 62.6%, while the traditional one is 40.8%. Meanwhile, the size of this novel Gysel power divider is only 25.5% of the traditional one when the same substrate is used and the same operating band is considered.
Somatic embryogenesis in conifers (1991)

Substantial progress has been made towards the development of systems for in vitro embryogenesis in conifers. Since the first report of somatic embryogenesis from zygotic embryos of Picea abies in 1985, cultured explants of at least 18 different coniferous species have been induced to produce somatic embryos. Somatic embryos have been cryopreserved, grown in liquid suspensions, and matured into plants. In addition, plantlets have been regenerated from protoplasts isolated from embryogenic suspensions of Picea glauca and Larix ×eurolepis, permitting studies into direct gene transfer and somatic hybridization. Currently however, it is only possible to obtain somatic embryogenesis from embryonic and juvenile explants. Furthermore, for most species the efficiency of plantlet production from somatic embryos is poor and remains a problem for the commercial utilization of this technology. Biochemical, cytological, and physiological studies of conifer somatic embryogenesis have resulted in improved knowledge concerning the origin of somatic embryos, storage product accumulation during embryo development, and similarities with zygotic embryos. Furthermore, the technique of indirect immunofluorescence microscopy has permitted investigations of the cytoskeleton in conifer cells and protoplasts, providing insights into cell division and morphogenesis. In this review, emphasis is placed on the more fundamental aspects of conifer somatic embryogenesis. Where possible, comparisons between zygotic and somatic embryogenesis are made. Key words: somatic embryogenesis, zygotic embryogenesis, conifers, review.
Assessment of Low-Power Laser Biostimulation on Chondral Lesions: An “In Vivo” Experimental Study (2000)

The purpose of this study was to evaluate whether intraoperative laser biostimulation can enhance healing of cartilaginous lesions of the knee. Surgery was performed on eighteen rabbits: a bilateral chondral lesion of 1.25 +/- 0.2 mm in length and 0.8 +/- 0.2 mm in width was created in the femoral medial condyle with a scalpel. The lesion in the left knee of each animal was treated intraoperatively using the diode Ga-Al-As 780nm. laser (300 Joules/cm2, 1 Watt, 300 Hertz, 10 minutes), while the right knee was left untreated, as control group. The animals were divided into three groups, A, B and C, according to the survival time after surgery, two, six and twelve weeks, respectively. The explants from the femoral condyles, both treated employing laser energy and left untreated, were examined histologically. Results showed a progressive filling with fibrous tissue of the cartilaginous lesion treated with laser irradiation, while no changes in the original lesion of the untreated group were observed at the end of the study. Maybe, in this experimental research, underexposure to laser irradiation was the cause for the absence of the necessary conditions for biostimulation.
Isolation and characterization of a somatomedin‐binding protein from mid‐term human amniotic fluid (1984)

Human amniotic fluid is rich in a binding protein for somatomedins. This binding protein competes with human placenta membranes for labelled somatomedin A. Consequently, the placenta radioreceptorassay for somatomedin can be used for detection of the binding protein. The protein was isolated from human amniotic fluid by a three-step procedure: First, stepwise ammonium sulphate precipitation; second, hydrophobic chromatography (phenyl-Sepharose); and third, anion-exchange chromatography (fast protein liquid chromatography). The total recovery of binding protein calculated with the placenta radioreceptorassay was 50%. Polyacrylamide gel electrophoresis under native and denaturating conditions of the isolated protein disclosed a single band. The relative molecular mass was 35000, determined by exclusion chromatography, and 32000 under denaturating conditions in sodium dodecyl sulphate/polyacrylamide gel electrophoresis. The isoelectric point was 4.3 according to chromatofocusing and the amino acid composition also disclosed a high content of acidic/amidated residues. The N-terminal amino acid sequence was Ala-Pro-Trp-Gln-Cys-Ala-Pro-Cys-Ser-Ala.
Chemical vapor deposition of tungsten oxide (1998)

Crystalline and amorphous thin films of tungsten(VI) oxide can be prepared by chemical vapor deposition using a variety of volatile precursors below 500 °C. Deposition parameters for preparation of WO3 films from tungsten hexacarbonyl [W(CO)6], tungsten hexafluoride (WF6), tungsten ethoxides [W(OEt)x, x = 5, 6] and tetra(allyl)tungsten [W(η3-C3H5)4] are summarized. The electrochromic behavior of these films is comparable with that observed for WO3 films prepared by evaporation, sputtering and electrodeposition. © 1998 John Wiley & Sons, Ltd.
Stability and Damped Critical Speeds of a Flexible Rotor in Fluid-Film Bearings (1974)

A method is described for calculating the threshold speed of instability and the damped critical speeds of a general flexible rotor in fluid-film journal bearings. It is analogous to the Myklestad-Prohl method for calculating critical speeds and is readily programmed for numerical computation. The rotor model can simulate any practical shaft geometry and support configuration. The bearings are represented by their linearized dynamic properties, also known as the stiffness and damping coefficients of the bearing, and the calculation includes hysteretic internal damping in the shaft and destabilizing aerodynamic forces. To demonstrate the application of the method, results are shown for an industrial, multistage compressor.
New selfalignment processes for amorphous silicon thin film transistors with polysilicon source and drain (1993)

New fabrication processes for selfaligned amorphous silicon TFTs are proposed. The TFTs have a polysilicon source and drain which are formed by ArF excimer laser annealing. They exhibit a field-effect mobility of 0.8 cm2/Vs, threshold voltage of 11 V, and on/off current ratio of higher than 106.
GPS Multipath Estimation and Mitigation Via Polarization Sensing Diversity: Parallel Iterative Cross Cancellation (2005)

Multipath estimation is affected by both the underlying signal to multipath ratio (SMR) and multipath to noise ratio (MNR). This paper proposes to strengthen the multipath signals for more accurate estimation before their ultimate removal. This is achieved by using a dual circularly polarized antenna. The GPS signal is right hand circularly polarized (RHCP). Upon reflection, it becomes left hand circularly polarized (LHCP) in most cases. Being matched to the RHCP signal, a GPS antenna has an inherent anti-multipath capability. This polarization discrimination, in conjunction with other antenna design features, typically offers a multipath attenuation from 3 ~ 10 dB. This amount of attenuation is not large enough to suppress the multipath signals to a harmless level in the carrier and code phase measurements, but makes the multipath parameter estimation more difficult in the presence of noise. With correlation functions available for both the RHCP and LHCP signals, the paper sets forth a mitigation technique based upon the cancellation of direct and multipath signals on the LHCP and RHCP correlations, respectively, in an iterative manner. The dual polarization architecture allows for parallel iterative cross cancellation (PICC), which is expected to outperform the sequential iterative cancellation (SIC) applied to the RHCP signal alone. Upon convergence, the RHCP signal is multipathfree allowing for accurate estimation of the direct signal timing. The direct signal can be removed from the LHCP signal, leaving clean multipath components for such applications as a multipath meter. Simulation results are presented to show the multipath cancellation performance as compared to the theoretical multipath error envelope and to the thermal noise errors under various SNR conditions for different multipath and polarization parameters. Sensitivity of the approach to the polarization discrimination factor is also evaluated.
Synthesis of 8-Substituted-10, 11-Dihydro-11-Oxo-5H-Dibenzo [b, e]-1 ,4-Diazepines (1978)

Chemical Laboratories, University of Ra.ja.stha.n, Ja.ipur (India.) <em>Manuscript received 18 September 19'17, revised 6 March 19'18, accepted 18 April 1978</em> 8-Amino-10,11-dibydro-11-oxo-5<em>H</em>-dibenzo[b, e]-1, 4-diazepine (1) synthesised by the cyclisation or 2', 4'-diaminodiphenylamine-1-carboxylic acid (1), was converted to its chloro- and iodo derivatives (3 and 4) by Sandmeyer reaction, and to bromo derivative (5) by Gattermann reaction.
Understanding the mechanism of action of bacterial superantigens from a decade of research (1999)

In the face of the unique diversity and plasticity of the immune system pathogenic organisms have developed multiple mechanisms in adaptation to their hosts, including the expression of a particular class of molecules called superantigens. Bacterial superantigens are the most potent stimulators of T cells. The functional consequences of the expression of superantigens by bacteria can be extended not only to T lymphocytes, but also to B lymphocytes and to cells of the myeloid compartment, including antigen-presenting cells and phagocytes. The biological effects of bacterial superantigens as well as their molecular aspects have now been studied for a decade. Although there is still a long way to go to clearly understand the role these molecules play in the establishment of disease, recently acquired knowledge of their biochemistry now offers unique experimental opportunities in defining the molecular rules of T-cell activation. Here, we present some of the most recent functional and molecular aspects of the interaction of bacterial superantigens with MHC class II molecules and the T-cell receptor.
Automated quantitative nephelometric latex immunoassay for determining ferritin in human serum (1992)

We describe a rapid and sensitive latex nephelometric immunoassay for quantifying ferritin in human serum. This latex immunoassay procedure uses commercially available ready-for-use reagents [Tina-Quant (a) Ferritin, Boehringer Mannheim] that have a long shelf life. The assay consists of incubating the diluted serum sample (5-fold) for 12 min at room temperature with latex particles covalently coated with anti-ferritin antibodies, and then quantifying the change of light-scatter produced. The assay is fully automated on the Behring nephelometer analyzer with a sampling rate of 150 samples/hour. The method has an analytical range of 3 to 260 micrograms/l. Maximal intra- and inter-assay CVs were 4.0 and 6.2%, respectively. Analytical recoveries ranged from 91.3 to 103.6%. Assay detection limit was less than 3 micrograms/l. Linearity of the test is given throughout the measuring range. There was no interference from bilirubin (up to 340 mumol/l), haemoglobin (up to 7 g/l), or rheumatoid factor (up to 1,100 IU/ml). Turbid and lipemic samples interfere. This interference may be avoided by pretreating these samples prior to assay. Results correlated well with those obtained by an automated ELISA test (r = 0.995) and with those of two commercial RIA methods (r greater than 0.97). This latex nephelometric procedure is a convenient method and represents an interesting alternative to other immunoassays for measuring ferritin in human serum.
Integrins Regulate Rac Targeting by Internalization of Membrane Domains (2004)

Translocation of the small GTP-binding protein Rac1 to the cell plasma membrane is essential for activating downstream effectors and requires integrin-mediated adhesion of cells to extracellular matrix. We report that active Rac1 binds preferentially to low-density, cholesterol-rich membranes, and specificity is determined at least in part by membrane lipids. Cell detachment triggered internalization of plasma membrane cholesterol and lipid raft markers. Preventing internalization maintained Rac1 membrane targeting and effector activation in nonadherent cells. Regulation of lipid rafts by integrin signals may regulate the location of membrane domains such as lipid rafts and thereby control domain-specific signaling events in anchorage-dependent cells.
Synthesis, molecular structure, and chemical behavior of hydrogen trans-bis(dimethyl sulfoxide)tetrachlororuthenate(III) and mer-trichlorotris(dimethyl sulfoxide)ruthenium(III): the first fully characterized chloride-dimethyl sulfoxide-ruthenium(III) complexes (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis, molecular structure, and chemical behavior of hydrogen trans-bis(dimethyl sulfoxide)tetrachlororuthenate(III) and mer-trichlorotris(dimethyl sulfoxide)ruthenium(III): the first fully characterized chloride-dimethyl sulfoxide-ruthenium(III) complexesEnzo Alessio, Gabriele Balducci, Mario Calligaris, Giacomo Costa, Wahib M. Attia, and Giovanni MestroniCite this: Inorg. Chem. 1991, 30, 4, 609–618Publication Date (Print):February 1, 1991Publication History Published online1 May 2002Published inissue 1 February 1991https://pubs.acs.org/doi/10.1021/ic00004a005https://doi.org/10.1021/ic00004a005research-articleACS PublicationsRequest reuse permissionsArticle Views2130Altmetric-Citations166LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Wnt2b controls retinal cell differentiation at the ciliary marginal zone (2003)

The ciliary marginal zone of the vertebrate retina contains undifferentiated progenitor cells that continue to proliferate and add new neurons and glia peripherally during the embryonic stages - even after the formation of a functional retina. To understand the molecular mechanism that controls the prolonged progenitor cell proliferation in the ciliary marginal zone, we employed a candidate molecule approach, focusing on Wnt2b (formerly know as Wnt13), which is expressed in the marginal most tip of the retina. Frizzled 4 and 5, seven-pass transmembrane Wnt receptors, were expressed in the peripheral and central part of the retina, respectively. LEF1, a downstream Wnt signaling component, was expressed at high levels in the ciliary marginal zone with expression gradually decreasing towards the central retina. The LEF1-expressing region, which is where Wnt signaling is supposedly activated, expressed a set of molecular markers that are characteristic of the progenitor cells in the ciliary marginal zone. Overexpression of Wnt2b by use of in ovo electroporation in the central retina inhibited neuronal differentiation and induced the progenitor cell markers. Blocking of the Wnt downstream signaling pathway by a dominant-negative LEF1 inhibited proliferation of the cells in the marginal area, which resulted in their premature neuronal differentiation. The progenitor cells in the ciliary marginal zone differentiated into all the neuronal and glial cell types when cultured in vitro, and they proliferated for a longer period than did centrally located progenitor cells that underwent a limited number of cell divisions. In addition, the proliferation of these progenitor cells was promoted in the presence of Wnt2b. These results suggest that Wnt2b functions to maintain undifferentiated progenitor cells in the ciliary marginal zone, and thus serves as a putative stem cell factor in the retina.
Molecular Bottlebrushes (1996)

Polymacromonomers consisting of oligostyrene side chains (700 ≤ Mn ≤ 5000 g/mol) and of a high molar mass polymethacrylate main chain are shown to exhibit a bottlebrush structure in that the PMA main chain adopts an extremely stiff conformation (i.e., a Kuhn statistical segment length of up to lk = 2000 Å) which is surrounded by the expanded but still flexible polystyrene side chains.