gebhart/patrepeval huggingface dataset:
Proteolytic Activation of the Haemagglutinin-Neuraminidase of Newcastle Disease Virus Involves Loss of a Glycopeptide (1980)

The uncleaved (HN0) and the cleaved (HN) forms of the haemagglutinin-neuraminidase glycoprotein of Newcastle disease virus (NDV), strain Ulster, were analysed by polyacrylamide gel electrophoresis under reducing and non-reducing conditions. When HN0 is converted into HN, a glycopeptide with an apparent mol. wt. of about 8000, which is not found in the mature spike, is removed.
Development of a Fully Automated Image Analysis Method for High Density cDNA and array CGH Microarray Based Genomic Studies (2013)

In this thesis, different techniques for image analysis of high density\nmicroarrays have been investigated. Most of the existing image analysis techniques\nrequire prior knowledge of image specific parameters and direct user intervention\nfor microarray image quantification. The objective of this research work was to\ndevelop of a fully automated image analysis method capable of accurately\nquantifying the intensity information from high density microarrays images. The\nmethod should be robust against noise and contaminations that commonly occur in\ndifferent stages of microarray development.
High oleic oils by selective hydrogenation of soybean oil (1969)

Abstract High oleic (monoene) oils were obtained from soybean oil by selective hydrogenation with copper catalysts. A mixture of nickel and copper chromite catalyst had activity suitable for producing the high monoene oils. A new catalyst (copper‐on‐Cab‐O‐Sil) prepared in the Laboratory was more active than commercial copper catalysts. Hydrogenated oils contained 61–72% monoenoic and 14–24% dienoic acids, and there was essentially no increase in stearic acid. The trans ‐isomer content of these oils varied between 17% to 32%. Double bonds in the monoene were distributed along the molecule from C 6 to C 15 , but were located preferentially in the C 9 position for the cis ‐monoene and in the C 10 and C 11 positions for the trans ‐monoene. When the iodine value of these high monoene oils was about 90–95, they remained liquid above 28 C. Citric acid treatment reduced the copper content of hydrogenated oils to a level that was comparable to that of the original soybean oil.
Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction (1989)

We applied the polymerase chain reaction to detection of the pathogenic protozoan Toxoplasma gondii based on our identification of a 35-fold-repetitive gene (the B1 gene) as a target. Using this procedure, we were able to amplify and detect the DNA of a single organism directly from a crude cell lysate. This level of sensitivity also allowed us to detect the B1 gene from purified DNA samples containing as few as 10 parasites in the presence of 100,000 human leukocytes. This is representative of the maximal cellular infiltration (10(5)/ml) in 1 ml of cerebrospinal fluid obtained from patients with toxoplasmic encephalitis. The B1 gene is present and conserved in all six T. gondii strains tested to date, including two isolates from patients with acquired immunodeficiency syndrome. No signal was detected by using this assay and DNAs from a variety of other organisms, including several which might be found in the central nervous system of an immunocompromised host. This combination of sensitivity and specificity should make detection of the B1 gene based on polymerase chain reaction amplification a very useful method for diagnosis of toxoplasmosis both in immunocompromised hosts and in congenitally infected fetuses.
Cell surface phenotype profiles distinguish stable and progressive chronic lymphocytic leukemia (2013)

Chronic lymphocytic leukemia (CLL) is clinically heterogeneous. While some patients have indolent disease for many years, 20-30% will progress and ultimately die of their disease. CLL may be classified by the Rai or Binet staging system, mutational status of the immunoglobulin variable heavy-chain gene (IGVH), ZAP-70 overexpression, cytogenetic abnormalities (13q-, + 12, 11q-, 17p-) and expression of several cell surface antigens (CD38, CD49d) that correlate with risk of disease progression. However, none of these markers identify all cases of CLL at risk. In a recent review, we summarized those CD antigens known to correlate with the prognosis of CLL. The present study has identified surface profiles of CD antigens that distinguish clinically progressive CLL from slow-progressive and stable CLL. Using an extended DotScan(™) CLL antibody microarray (Version 3; 182 CD antibodies), and with refined analysis of purified CD19 + B-cells, the following 27 CD antigens were differentially abundant for progressive CLL: CD11a, CD11b, CD11c, CD18, CD19, CD20 (two epitopes), CD21, CD22, CD23, CD24, CD25, CD38, CD40, CD43, CD45, CD45RA, CD52, CD69, CD81, CD84, CD98, CD102, CD148, CD180, CD196 and CD270. The extensive surface profiles obtained provide disease signatures with an accuracy of 79.2%, a sensitivity of 83.9% and a specificity of 72.5% that could provide the basis for a rapid test to triage patients with CLL according to probability of clinical progression and potential earlier requirement for treatment.
Bicycling Science (2004)

A new, updated edition of a popular book on the history, science, and engineering of bicycles.The bicycle is almost unique among human-powered machines in that it uses human muscles in a near-optimum way. This new edition of the bible of bicycle builders and bicyclists provides just about everything you could want to know about the history of bicycles, how human beings propel them, what makes them go faster, and what keeps them from going even faster. The scientific and engineering information is of interest not only to designers and builders of bicycles and other human-powered vehicles but also to competitive cyclists, bicycle commuters, and recreational cyclists.The third edition begins with a brief history of bicycles and bicycling that demolishes many widespread myths. This edition includes information on recent experiments and achievements in human-powered transportation, including the "ultimate human- powered vehicle," in which a supine rider in a streamlined enclosure steers by looking at a television screen connected to a small camera in the nose, reaching speeds of around 80 miles per hour. It contains completely new chapters on aerodynamics, unusual human-powered machines for use on land and in water and air, human physiology, and the future of bicycling. This edition also provides updated information on rolling drag, transmission of power from rider to wheels, braking, heat management, steering and stability, power and speed, and materials. It contains many new illustrations.
Effects of cholesterol depletion by cyclodextrin on the sphingolipid microdomains of the plasma membrane (1998)

Sphingolipid microdomains are thought to result from the organization of plasma membrane sphingolipids and cholesterol into a liquid ordered phase, wherein the glycosylphosphatidylinositol (GPI)-anchored proteins are enriched. These domains, resistant to extraction by cold Triton X-100, can be isolated as buoyant membrane complexes (detergent-resistant membranes) in isopycnic density gradients. Here the effects of methyl-beta-cyclodextrin (MBCD), a specific cholesterol-binding agent that neither binds nor inserts into the plasma membrane, were investigated on the sphingolipid microdomains of lymphocytes. MBCD released substantial quantities of GPI-anchored Thy-1 and glycosphingolipid GM1, and also other surface proteins including CD45, and intracellular Lck and Fyn kinases. From endothelial cells, MBCD released GPI-anchored CD59, and CD44, but only a negligible amount of caveolin. Most MBCD-released Thy-1 and CD59 were not sedimentable and thus differed from Thy-1 released by membrane-active cholesterol-binding agents such as saponin and streptolysin O, or Triton X-100. Unlike that released by Triton X-100, only part of the Thy-1 molecules released by MBCD was buoyant in density gradients and co-isolated with GM1. Finally, treatment of Triton X-100-isolated detergent-resistant membranes with MBCD extracted most of the cholesterol without affecting the buoyant properties of Thy-1 or GM1. We suggest that (1) MBCD preferentially extracts cholesterol from outside, rather than within the sphingolipid microdomains and (2) this partly solubilizes GPI-anchored and transmembrane proteins from the glycerophospholipid-rich membrane and releases sphingolipid microdomains in both vesicular and non-vesicular form.
Methods for the Construction of the Indolizine Nucleus* (1976)

Methods for the synthesis of various types of indolizines and a characteristic selection of their aza analogues are described. The reactions involved are classified as (a) condensation reactions, (b) 1,3-dipolar cycloadditions, and (c) 1,5-dipolar cyclizations. 1. Synthesis of Indolizines by Condensation Reactions 1.1. Reactions of 2-Methylpyridine and its Derivatives with Acid Anhydrides (Scholtz Reaction) 1.2. Cyclizations of Quaternary Pyridinium Salts (Tschitschibabin Reaction) 1.3. Cyclizations of 3-(2-Pyridyl)-1-propanols and their Derivatives 1.4. Reactions of Heteroaromatic Nitrogen Compounds with Acetylenic and Olefinic Compounds 1.5. Miscellaneous Condensations 2. Synthesis of Indolizines by 1,3-Dipolar Cycloadditions 2.1. Reactions of Pyridinium and Related Heteroaromatic Ylids 2.2. Reactions of N-Iminopyridinium Ylids 2.3. Reactions of Miscellaneous 1,3-Dipoles 3. Synthesis of Indolizines by 1,5-Dipolar Cyclizations
New Method for Specifying Color Rendering Properties of Light Sources based on the Feeling of Contrast (1995)

“Visual Clarity” or “Brightness Sensation” of colored objects changes by changing the color rendering properties of light sources. Though visual clarity is considered one of the most important characteristics of the color rendering properties of light sources, it can not be estimated adequately by using the present CIE Ra method. For this reason, we studied the cause of visual clarity and confirmed that visual clarity was caused by the feeling of contrast between the colored objects under the illumination. In addition, we proposed the method for predicting the illuminances for the same feeling of contrast under various light sources, based on the gamut area made by the four component colors of a four-color combination in a brightness and colorfulness space.The purpose of the present study is, furthermore, to develop an estimation method of the feeling of contrast under test light sources and to propose a new index of the feeling of contrast under a test light source by using a simple transformation of the gamut area derived under the light source. The new proposed index correlated well with the illuminance ratio for equal feeling of contrast (or equal visual clarity) on various light sources reported so far. The new index is compared with the other indices, such as General Color Rendering Index Ra, Color-Discrimination Index and Flattery Index, and it is confirmed that the new index is a different measuring index from these indices. By using the new method proposed in this paper together with the present CIE Ra method, the color rendering properties of light sources can be well estimated from various points of view.
Butyrate inhibits seeding and growth of colorectal metastases to the liver in mice (1996)

These studies (1) show that butyrate inhibits seeding and growth of hepatic colorectal metastases in vivo, (2) associate butyrate's antiproliferative effects with inhibition of mevalonate-mediated cell growth, and (3) indicate that 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors may have synergistic antiproliferative effects when combined with butyrate.
Change in practice (1992)

Pauline Smith outlines how one elderly care unit set about monitoring the incidence of pressure sores and the use of equipment and products
Reprogramming MHC specificity by CRISPR-Cas9-assisted cassette exchange (2017)

The development of programmable nucleases has enabled the application of new genome engineering strategies for cellular immunotherapy. While targeted nucleases have mostly been used to knock-out or knock-in genes in immune cells, the scarless exchange of entire immunogenomic alleles would be of great interest. In particular, reprogramming the polymorphic MHC locus could enable the creation of matched donors for allogeneic cellular transplantation. Here we show a proof-of-concept for reprogramming MHC-specificity by performing CRISPR-Cas9-assisted cassette exchange. Using murine antigen presenting cell lines (RAW264.7 macrophages), we demonstrate that the generation of Cas9-induced double-stranded breaks flanking the native MHC-I H2-Kd locus led to exchange of an orthogonal H2-Kb allele. MHC surface expression allowed for easy selection of reprogrammed cells by flow cytometry, thus obviating the need for additional selection markers. MHC-reprogrammed cells were fully functional as they could present H2-Kd-restricted peptide and activate cognate T cells. Finally, we investigated the role of various donor template formats on exchange efficiency, discovering that templates that underwent in situ linearization resulted in the highest MHC-reprogramming efficiency. These findings highlight a potential new approach for the correcting of MHC mismatches in cellular transplantation.
Quantitative trait loci affecting growth and carcass composition of cattle segregating alternate forms of myostatin. (2000)

The effects of the bovine myostatin gene on chromosome 2 on birth and carcass traits have been previously assessed. The objective of this study was to identify additional quantitative trait loci (QTL) for economically important traits in two families segregating an inactive copy of myostatin. Two half-sib families were developed from Belgian Blue x MARC III (n = 246) and Piedmontese x Angus (n = 209) sires. Traits analyzed were birth (kg) and yearling weight (kg); hot carcass weight (kg); fat depth (cm); marbling score; longissimus muscle area (cm2); estimated kidney, pelvic, and heart fat (%); USDA yield grade; retail product yield (%); fat yield (%); and wholesale rib-fat yield (%). Meat tenderness was measured as Warner-Bratzler shear force at 3 and 14 d postmortem. The effect of myostatin on these traits was removed by using phase information obtained from the previous study with six microsatellite markers flanking the locus. Selective genotyping was done on 92 animals from both families to identify genomic regions potentially associated with retail product yield and fat depth, using a total of 150 informative markers in each family. Regions in which selective genotyping indicated the presence of QTL were evaluated further by genotyping the entire population and additional markers. For the family with Belgian Blue inheritance (n = 246), a significant QTL for birth and yearling weight was identified on chromosome 6. Suggestive QTL were identified for longissimus muscle area and hot carcass weight on chromosome 6 and for marbling on chromosomes 17 and 27. For the family with Piedmontese inheritance (n = 209), suggestive QTL on chromosome 5 were identified for fat depth, retail product yield, and USDA yield grade and on chromosome 29 for Warner-Bratzler shear force at 3 and 14 d postmortem. Interactions suggesting the presence of QTL were observed between myostatin and chromosome 5 for Warner-Bratzler shear force at 14 d postmortem and between myostatin and chromosome 14 for fat depth. Thus, in families segregating an inactive copy of myostatin in cattle, other loci influencing quantitative traits can be detected. These results are the initial effort to identify and characterize QTL affecting carcass and growth traits in families segregating myostatin.
Title&gt;Additive cytotoxicity of different monoclonal antibody-cobra venom factor conjugates for human neuroblastoma cells (1997)

Insufficient numbers of antigen molecules and heterogeneity of antigen expression on tumor cells are major factors limiting the immunotherapeutic potential of the few clinically useful monoclonal antibodies capable of mediating complement cytotoxicity and antibody-dependent cellular cytotoxicity. To overcome this limitation, we converted two non-cytotoxic monoclonal anti-neuroblastoma antibodies, designated 3E7 (IgG2b) and 8H9 (IgG1), and the non-cytotoxic F(ab')2 fragment of the cytotoxic monoclonal anti-GD2 antibody 3F8 (IgG3) into cytotoxic antibody conjugates by covalent attachment of cobra venom factor (CVF), a structural and functional homologue of the activated third component of complement. Competitive binding experiments confirmed the different specificities of the three antibodies. In the presence of human complement, all three antibody-CVF conjugates mediated selective complement-dependent lysis of human neuroblastoma cells. Consistent with the kinetics of the alternative pathway of complement, approximately seven hours incubation were required to reach maximum cytotoxicity of up to 25% for the 3E7-CVF conjugate, up to 60% for the 8H9-CVF conjugate, and up to 95% for the 3F8 F(ab')2-CVF conjugate. The different extent of maximal cytotoxic activity of the three conjugates was reflected by corresponding differences in the extent of binding of both unconjugated antibodies and the respective conjugates. Any combination of the three antibody-CVF conjugates caused an additive effect in complement-mediated lysis. Using a cocktail of all three conjugates, the extent of complement-mediated killing could be increased up to 100%. These data demonstrate that by coupling of CVF the relative large number of non-cytotoxic monoclonal anti-tumor antibodies of interesting specificity can be used to design cocktails of cytotoxic conjugates and, thereby, to overcome the problem of insufficient and heterogeneous antigen expression on tumor cells for immunotherapy.
Tunable laboratory extended x-ray absorption fine structure system (1980)

A new sensitive x-ray monochromator and detector system for performing extended x-ray absorption fine structure (EXAFS) measurements in the laboratory is described. The monochromator combines x-ray focusing optics with rapid elemental tunability. The detection system effectively removes glitches from the data stream, regardless of whether they are due to impurity lines from the x-ray source or if they are due to random instabilities in the incident beam. Used together with a high intensity rotating anode x-ray source, this system can provide synchrotronlike photon intensities, flexibility and resolution, with the easy access and control possible only in the laboratory.
Linkage of a Gene Causing Familial Membranoproliferative Glomerulonephritis Type III to Chromosome 1 (2002)

Membranoproliferative glomerulonephritis (MPGN) type III is a chronic progressive renal disease of unknown cause. The diagnosis is based on renal pathologic features (specifically immunofluorescence staining patterns and ultrastructural appearance). Mesangial cell proliferation and subendothelial and subepithelial deposits characterize the renal disease. Although the actual prevalence of this disease is not known, the disease is rare and usually sporadic. The clinical features of MPGN include the nephrotic syndrome and hematuria, with renal dysfunction occurring in approximately 50% of patients. Progression to end-stage renal disease is variable, and some patients exhibit stabilization or even improvement. Here is presented an Irish family in which there are eight affected members in four generations, suggesting autosomal dominant inheritance. This is the only reported family with an inherited form of MPGN type III. To evaluate the disease in this family, a genome-wide scan was performed with a panel of 402 polymorphic microsatellite markers, defining a grid with an average resolution of 10 cM (centimorgans). Significant evidence for linkage was observed on chromosome 1q31-32, with a maximal logarithm of the odds score of 3.86 at theta = 0.00 for microsatellite marker GATA135F02. Recombination events among affected individuals, as detected by haplotype analysis, established a 22-cM minimal candidate region flanked by markers D1S3470 and GATA124F08. The data provide evidence for a gene for familial MPGN on chromosome 1q.
Staircase lens: a binary and diffractive field curvature corrector (1993)

The optical characteristics of a family of diffractive lenses, called staircase lenses, are discussed. These lenses contribute no optical power and the fourth-order wave aberrations Petzval field curvature, distortion, and chromatic aberrations when collimated illumination is used. A diamond-turned staircase lens comprising 150 steps was manufactured. The test results verify the generation of field curvature and longitudinal chromatic aberration.
Lack of PTEN expression in non-small cell lung cancer could be related to promoter methylation. (2002)

Although genetic alterations of the PTEN gene are rare in NSCLC, loss of PTEN protein is not an uncommon event in early-stage NSCLC. Lack of PTEN expression may be partially explained by promoter methylation.
Cytokines and Sickness Behavior (1998)

Peripheral and central injections of interleukin-1 (IL-1) and lipopolysaccharide (LPS) induce the expression of proinflammatory cytokines in the brain and have profound depressing effects on spontaneous and learned behaviors. These effects are mediated by vagal afferents, because they are abrogated by section of the vagus nerves at the subdiaphragmatic level in rats and mice. Vagotomy does not interfere with the synthesis and release of proinflammatory cytokines at the periphery, because plasma and tissue levels of interleukin-1 of vagotomized animals are similar to those of sham-operated animals. Furthermore, the consequences of vagotomy on the host behavioral response to peripheral cytokines are specific to the intraperitoneal route of administration of cytokines because vagotomized animals are still able to respond to IL-1 injected intravenously, subcutaneously, and into the lateral ventricle of the brain. Finally, substance P and cholecystokinin do not appear to play a key role in the transmission of the immune message to the brain because pretreatment by capsaicin or by specific antagonists of CCKA and CCKB receptors does not alter the behavioral effects of LPS and IL-1. All these findings point to the role of neural afferents for transmitting the immune message from the periphery to the brain.
Memory and information processing capacity after closed head injury. (1981)

The relation between duration of post-traumatic amnesia (PTA), performance on memory tests, and the Paced Auditory Serial Addition Task (PASAT) was examined in two samples of young adult closed head injury patients. Three different effects were isolated: (1) an attention and concentration factor, related to PASAT scores, (2) a deficit in the ability to place material into long-term memory storage, related to PTA durations, and (3) an impairment in the ability to retrieve material once it has been stored, which was not predicted by either PTA or PASAT.