gebhart/patrepeval huggingface dataset:
The Osmotic Potential of Polyethylene Glycol 6000 (1973)

Osmotic potential (psi(s)) of aqueous solutions of polyethylene glycol 6000 (PEG-6000) was curvilinearly related to concentration. At given concentrations, psi(s) increased linearly with temperature. The effects of concentration and temperature on psi(s) of PEG-6000 solutions differ from those for most salts and sugars and apparently are related to structural changes in the PEG polymer. Measurements of psi(s) with thermocouple psychrometers are more negative than those with a vapor pressure osmometer, with the psychrometer probably giving the more nearly correct psi(s) for bulk solutions. An empirical equation permits calculation of psi(s) from known concentrations of PEG-6000 over a temperature range of 15 to 35 C. Viscometery and gravimetric analysis are convenient methods by which the concentrations of PEG-6000 solutions may be measured.
Using Targeted Sequencing of Paralogous Sequences for Noninvasive Detection of Selected Fetal Aneuploidies (2016)

This study introduces a novel NIPT aneuploidy detection approach using targeted sequencing of paralog motifs and establishes proof-of-concept for a potentially low-cost, highly scalable method for the identification of selected fetal aneuploidies with performance and nonreportable rate similar to other published methods.
The impact of microemboli during cardiopulmonary bypass on neuropsychological functioning. (1994)

These data suggest that neuropsychological deficits after routine cardiopulmonary bypass are related to the number of microemboli delivered during surgery. Furthermore, the numbers of microemboli may be reduced by including a 40-microns filter on the arterial line.
Plasma levels of B-lymphocyte stimulator increase with HIV disease progression (2003)

We measured the plasma levels of B-lymphocyte stimulator (BLyS) in 101 HIV-1-infected patients and 18 controls. BLyS levels were higher among HIV-positive patients [median 5.70 (3.90) versus 4.62 (1.04) ng/ml, P = 0.002], who had significantly higher BLyS and total serum globulin levels with decreasing CD4 cell counts. Moreover, BLyS levels increased exponentially below 100 CD4 cells/microl. BLyS and globulin levels increase as HIV dis ease progresses, suggesting a role for BLyS in the hypergammaglobulinemia of HIV infection.
Interleukin-1α and Interleukin-6 Enhance the Antibacterial Properties of Cultured Composite Keratinocyte Grafts (2002)

Treatment with interleukin-1alpha or interleukin-6 of cultured composite keratinocyte grafts stimulates the innate immune response of keratinocytes, enhances the antibacterial properties of these grafts, and may better prepare them to combat infections in contaminated burn wounds.
Adoptive cellular therapy: A race to the finish line (2015)

Adoptive T cell transfer for cancer, chronic infection, and autoimmunity is an emerging field that shows promise in recent trials. Using the principles of synthetic biology, advances in cell culture and genetic engineering have made it possible to generate human T cells that display desired specificities and enhanced functionalities compared with the natural immune system. The prospects for widespread availability of engineered T cells have changed dramatically, given the recent entry of the pharmaceutical industry to this arena. Here, we discuss some of the challenges--such as regulatory, cost, and manufacturing--and opportunities, including personalized gene-modified T cells, that face the field of adoptive cellular therapy.
Differential Expression of Wnt Genes in Normal and Flat Variants of PC 12 Cells, a Cell Line Responsive to Ectopic Wnt1 Expression (1998)

Wnt genes encode secreted growth factor-like proteins that participate in growth regulation, differentiation and tumorigenesis. Ectopic expression of Wnt1 converts the PC12 neural crest-derived rat pheochromocytoma cell line from a round phenotype that express chromaffin markers to flat adherent cells (termed PC12/Wnt1) that do not express them. A pool of spontaneously flat variants of PC12 cells (PC12/flat) is phenotypically similar to the PC12/Wnt1 cells, but does not express Wnt1. Here we describe the expression of 13 Wnt genes in wild type PC12, PC12/flat and PC12/Wnt1 cells. Wild type PC12 expressed Wnt3, Wnt3a, Wnt4, Wnt5a, Wnt6, Wnt10a and Wnt11. Compared with expression in wild type cells, both PC12/flat and PC12/Wnt1 cells lost most or all expression of Wnt3a and Wnt4 and gained expression of Wnt7b. Wnt5a and Wnt6 expression was higher in PC12/Wnt1 cells than in PC12 or PC12/flat. Wnt3 was expressed at low levels in both PC12 and PC12/flat, but was absent in PC12/Wnt1 cells. Wnt10a and Wnt11 were approximately equally expressed in the three groups, and Wnt2, Wnt5b, Wnt7a, Wnt10b and endogenous Wnt1 mRNAs were not detected. These results demonstrate that the expression of some Wnt genes changes in PC12 cells upon conversion to the flat phenotype, and suggest that Wnt1 may modulate expression of several other Wnt genes in these cells.
Optimization for high-density cultivation of heterotrophic Chlorella based on a hybrid neural network model (2006)

The high cell density and productivity of biomass obtained in this study is of significance for the commercial cultivation of Chlorella. The simple and efficient optimization strategy proposed in this paper may be employed in heterotrophic mass culture of Chlorella as well as other similar organisms.
Pharmacokinetic modulation of irinotecan and metabolites by cyclosporin A. (1996)

The focus of this investigation was to modulate the pharmacokinetics of irinotecan and its metabolites, SN-38 and SN-38G, by possibly reducing biliary excretion, which in turn could lower irinotecan toxicity. We determined the effect of a known cholestatic agent, cyclosporin A (CsA), which is transported across the bile canalicular membrane by P-glycoprotein, on the biliary excretion of irinotecan and its metabolites. Wistar rats were pretreated with 60 mg/kg CsA 5 min before an i.v. dose of irinotecan at dose levels of 6, 10, and 20 mg/kg. The control groups received irinotecan only. CsA pretreatment resulted in an average increase of 339, 361, and 192% in the area under the plasma concentration-time curve of irinotecan, SN-38, and SN-38G, respectively. Analysis of clearance (CL) of irinotecan indicated a 55 and 81% reduction in the average renal and nonrenal CLs, respectively, in the pretreated groups. The nonrenal CL, which is the primary component of irinotecan CL, includes protein and tissue binding as well as the metabolic and biliary CL of irinotecan. There was no change in the volume of distribution at steady state (indicative of unchanged binding) and in the metabolic conversion of irinotecan to SN-38 due to pretreatment. Therefore, the significant reduction in the systemic CL of irinotecan due to CsA pretreatment was primarily due to lowered biliary excretion. Studies using a photoaffinity analogue of verapamil, [125I]NAS-VP, and membrane vesicles from the multidrug-resistant cell line, MCF-7/Adr, revealed that irinotecan and metabolites had moderate interaction with P-glycoprotein. Further studies are required to determine the mechanism of inhibitory effect of CsA on the biliary excretion of irinotecan and its metabolites.
Unsupervised personal name disambiguation (2003)

This paper presents a set of algorithms for distinguishing personal names with multiple real referents in text, based on little or no supervision. The approach utilizes an unsupervised clustering technique over a rich feature space of biographic facts, which are automatically extracted via a language-independent bootstrapping process. The induced clustering of named entities are then partitioned and linked to their real referents via the automatically extracted biographic data. Performance is evaluated based on both a test set of handlabeled multi-referent personal names and via automatically generated pseudonames.
Interaction of a Novel GDP Exchange Inhibitor with the Ras Protein (1998)

Mutated, tumorigenic Ras is present in a variety of human tumors. Compounds that inhibit tumorigenic Ras function may be useful in the treatment of Ras-related tumors. The interaction of a novel GDP exchange inhibitor (SCH-54292) with the Ras-GDP protein was studied by NMR spectroscopy. The binding of the inhibitor to the Ras protein was enhanced at low Mg2+ concentrations, which enabled the preparation of a stable complex for NMR study. To understand the enhanced inhibitor binding and the increased GDP dissociation rates of the Ras protein, the conformational changes of the Ras protein at low Mg2+ concentrations was investigated using two-dimensional 1H-15N HSQC experiments. The Ras protein existed in two conformations in slow exchange on the NMR time scale under such conditions. The conformational changes mainly occurred in the GDP binding pocket, in the switch I and the switch II regions, and were reversible. The Ras protein resumed its regular conformation after an excess amount of Mg2+ was added. A model of the inhibitor in complex with the Ras-GDP protein was derived from intra- and intermolecular NOE distance constraints, and revealed that the inhibitor bound to the critical switch II region of the Ras protein.
Preparation and bivariate analysis of suspensions of human chromosomes (1985)

Chromosomes were isolated from a variety of human cell types using a HEPES-buffered hypotonic solution (pH 8.0) containing KCl, MgSO4, dithioerythritol, and RNase. The chromosomes isolated by this procedure could be stained with a variety of fluorescent stains including propidium iodide, chromomycin A3, and Hoechst 33258. Addition of sodium citrate to the stained chromosomes was found to improve the total fluorescence resolution. High-quality bivariate Hoechst vs. chromomycin fluorescence distributions were obtained for chromosomes isolated from a human fibroblast cell strain, a human colon carcinoma cell line, and human peripheral blood lymphocyte cultures. Good flow karyotypes were also obtained from primary amniotic cell cultures. The Hoechst vs. chromomycin flow karyotypes of a given cell line, made at different times and at dye concentrations varying over fourfold ranges, show little variation in the relative peak positions of the chromosomes. The size of the DNA in chromosomes isolated using this procedure ranges from 20 to over 50 kilobases. The described isolation procedure is simple, it yields high-quality flow karyotypes, and it can be used to prepare chromosomes from clinical samples.
Hunter gatherer (2002)

Task analysis of how users collect information from within Web pages indicates that while capturing information within-Web-page is a common task, it is not a frequent one. Tool support for this interaction is poor: users must move between browsers for copying and editors for pasting content They must also name the components captured and remember to copy and add the URL from the source. These subtasks force users away from their primary focus of information gathering and into information management. Hunter Gatherer is a browser-based tool designed to address the specific problems of forced divided attention in information gathering smaller-than-Web-page sized components.
Potent non-6-fluoro-substituted quinolone antibacterials: synthesis and biological activity (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPotent non-6-fluoro-substituted quinolone antibacterials: synthesis and biological activityBenoit Ledoussal, Daniel Bouzard, and Evangelos CoroneosCite this: J. Med. Chem. 1992, 35, 1, 198–200Publication Date (Print):January 1, 1992Publication History Published online1 May 2002Published inissue 1 January 1992https://pubs.acs.org/doi/10.1021/jm00079a028https://doi.org/10.1021/jm00079a028research-articleACS PublicationsRequest reuse permissionsArticle Views489Altmetric-Citations38LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
STAT3 Serine Phosphorylation by ERK-Dependent and -Independent Pathways Negatively Modulates Its Tyrosine Phosphorylation (1997)

Recent studies have indicated that serine phosphorylation regulates the activities of STAT1 and STAT3. However, the kinase(s) responsible and the role of serine phosphorylation in STAT function remain unresolved. In the present studies, we examined the growth factor-dependent serine phosphorylation of STAT1 and STAT3. We provide in vitro and in vivo evidence that the ERK family of mitogen-activated protein (MAP) kinases, but not JNK or p38, specifically phosphorylate STAT3 at serine 727 in response to growth factors. Evidence for additional mitogen-regulated serine phosphorylation is also provided. STAT1 is a relatively poor substrate for all MAP kinases tested both in vitro and in vivo. STAT3 serine phosphorylation, not its tyrosine phosphorylation, results in retarded mobility of the STAT3 protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Importantly, serine 727 phosphorylation negatively modulates STAT3 tyrosine phosphorylation, which is required for dimer formation, nuclear translocation, and the DNA binding activity of this transcriptional regulator. Interestingly, the cytokine interleukin-6 also stimulates STAT3 serine phosphorylation, but in contrast to growth factors, this occurs by an ERK-independent process.
Investigation of Antimony Oxide Films Deposited by Atomic Layer Deposition (2012)

Atomic layer deposition of antimony oxide thin films was investigated in a temperature range between 50 and 250 °C using triethylantimony and ozone. Layer thickness was measured by spectroscopic ellipsometry using adapted layer models. It was proved that homogenous deposition could be achieved on flat silicon wafers and in trench structures. Little growth was found below 100 °C deposition temperature and linear temperature dependence on the growth rate between 100 and 250 °C. High-resolution transmission electron microscope measurements of as-deposited films showed the existence of SiO2 interface layer and crystalline grains within the antimony oxide. The thermal stability of antimony oxide layers was investigated by rapid thermal annealing experiments in the temperature range of 500 to 850 °C. The layers were not stable above 700 °C, which was assumed to be the reason for failing to apply this material as dopant source for ultra-shallow antimony doping of silicon so far.
Conditional Knock-out of Integrin-linked Kinase Demonstrates an Essential Role in Protein Kinase B/Akt Activation (2003)

Protein kinase B (PKB/Akt) plays a pivotal role in signaling pathways downstream of phosphatidylinositol 3-kinase, regulating fundamental processes such as cell survival, cell proliferation, differentiation, and metabolism. PKB/Akt activation is regulated by phosphoinositide phospholipid-mediated plasma membrane anchoring and by phosphorylation on Thr-308 and Ser-473. Whereas the Thr-308 site is phosphorylated by PDK-1, the identity of the Ser-473 kinase has remained unclear and controversial. The integrin-linked kinase (ILK) is a potential regulator of phosphorylation of PKB/Akt on Ser-473. Utilizing double-stranded RNA interference (siRNA) as well as conditional knock-out of ILK using the Cre-Lox system, we now demonstrate that ILK is essential for the regulation of PKB/Akt activity. ILK knock-out had no effect on phosphorylation of PKB/Akt on Thr-308 but resulted in almost complete inhibition of phosphorylation on Ser-473 and significant inhibition of PKB/Akt activity, accompanied by significant stimulation of apoptosis. The inhibition of PKB/Akt Ser-473 phosphorylation was rescued by kinase-active ILK but not by a kinase-deficient mutant of ILK, suggesting a role for the kinase activity of ILK in the stimulation of PKB/Akt phosphorylation. ILK knock-out also resulted in the suppression of phosphorylation of GSK-3beta on Ser-9 and cyclin D1 expression. These data establish ILK as an essential upstream regulator of PKB/Akt activation.
Excimer Emission and Wavelength Control from Light-Emitting Diodes Based on Side-Chain Polymers (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTExcimer Emission and Wavelength Control from Light-Emitting Diodes Based on Side-Chain PolymersJ. Bisberg, W. J. Cumming, R. A. Gaudiana, K. D. Hutchinson, R. T. Ingwall, E. S. Kolb, P. G. Mehta, R. A. Minns, and C. P. PetersenCite this: Macromolecules 1995, 28, 1, 386–389Publication Date (Print):January 1, 1995Publication History Published online1 May 2002Published inissue 1 January 1995https://pubs.acs.org/doi/10.1021/ma00105a056https://doi.org/10.1021/ma00105a056research-articleACS PublicationsRequest reuse permissionsArticle Views343Altmetric-Citations56LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Electrospun Polyacrylonitrile Nanofibers Containing a High Concentration of Well-Aligned Multiwall Carbon Nanotubes (2005)

Composite nanofiber sheets of well-aligned polyacrylonitrile nanofibers (PAN) containing multiwall carbon nanotubes (MWCNTs) were prepared by electrospinning a MWCNT-suspended solution of PAN in dimethyl formamide using a moving collector. Scanning electron microscopy, atomic force microscopy, transmission electron microscopy (TEM), IR spectroscopy, Raman spectroscopy, X-ray scattering, and the Instron test were used to characterize the nanofiber sheets. TEM observation showed the MWCNTs were parallel and oriented along the axes of the nanofibers. The mechanical properties of the composite nanofibers were reinforced by MWCNT fillers. Carbonization processes showed that a higher concentration of MWCNTs effectively resisted heat shrinkage of the composite nanofiber sheet.
Diffractive Optical Elements For Use In Infrared Systems (1989)

Diffractive optical elements have the potential to improve the performance of infrared optical systems. An approach to constructing high quality diffractive elements has been developed using standard integrated circuit techniques. It is possible to implement arbitrary phase profiles since the elements are computer generated.
Design and construction of a wheel-directly-coupled axial-flux PM motor prototype for EVs (2002)

In electric vehicle (EV) motor drives, the use of a low-speed motor coupled directly to the wheel axle allows a reduction of the vehicle weight and an improvement in the drive efficiency. Slotless axial-flux PM motors are particularly suited for such an application, since they can be designed for high torque-to-weight ratio and efficiency. This paper deals with a 16 poles axial-flux PM motor prototype which is used in the propulsion drive of an electrical scooter. The motor prototype has 45 Nm peak torque, 6.8 kg active materials weight, and is coupled directly to the scooter rear wheel. The paper discusses design and construction of the motor prototype, and reports experimental results achieved from laboratory tests. Finally, details concerning the arrangement of the scooter motor drive are given.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Neural differentiation of rhesus embryonic stem cells (1998)

Primate embryonic stem (ES) cells are capable of indefinite, undifferentiated proliferation and maintain the potential to differentiate to trophoblast and derivatives of embryonic endoderm, mesoderm, and ectoderm. We previously reported that neural differentiation by rhesus ES cells in teratomas includes tissue with a remarkable resemblance to neural tube (Thomson et al. 1995). Here we examine a series of markers including a cell proliferation marker, neurofilament proteins, and glial fibrillary acidic protein (GFAP) in teratomas at 5, 6, 7, 9, and 12 weeks after rhesus ES cell transplantation into muscles of immunodeficient mice. All teratomas examined contained derivatives of all three embryonic germ layers. Neural differentiation included tissues resembling neural tube and embryonic ganglia, as well as individual dispersed neurons, and brain-like gray matter. Tumours of all ages contained neurons and proliferating cells, indicated by staining for neurofilament subunits and Ki67 antigens. Younger tumours contained no or few astrocytes indicated by the absence of GFAP staining, but as these tumours developed, there was an increase in astrocyte differentiation. The results indicate that normal neural differentiation is recapitulated, in part, by the differentiation of rhesus ES cells in teratomas. The differentiation of rhesus ES cells provides an important new model for understanding human neural differentiation.
Stable, soluble T-cell receptor molecules for crystallization and therapeutics (2003)

Antibody and T-cell receptors (TCRs) are the primary recognition molecules of the adaptive immune system. Antibodies have been extensively characterized and are being developed for a large number of therapeutic applications. This has been possible because of the ability to manufacture stable, soluble, monoclonal antibodies which retain the antigen specificity of B cells. Unlike antibodies, TCRs are not expressed in a soluble form, but are anchored to the T-cell surface by an insoluble trans-membrane domain. Characterization and development of TCRs has been hampered by the lack of suitable methods for producing them as soluble and stable proteins. Here we report the engineering of soluble human TCRs suitable for crystallization studies and potentially for in vivo therapeutic use.
E-learning in diverse cultural contexts (2006)

e-learning provides a relatively new method for teaching in higher education in general and in East and South East Asia in particular. Many e-learning programmes are developed using a western based pedagogy for use in the east.However, the evaluation of these programmes is in its infancy. The current study was co-funded by the EU and adds to the body of research providing guidance for e-educators. The qualitative data collected from e learning tutors from South East and East Asia indicates that there are differences based on cultural context in the following areas of communication; within student peer groups; tutor to student, as well as variations in the willingness to discuss problems. The study provides recommendations to enhance the distance education programmes. The conclusion points to issues that should be addressed at the design and managerial levels.