gebhart/patrepeval huggingface dataset:
CD11b+ Cells are the Major Source of Oxidative Stress in UV Radiation-irradiated Skin: Possible Role in Photoaging and Photocarcinogenesis¶ (2003)

Exposure of skin to solar UV radiation induces oxidative stress and suppression of cell-mediated immune responses. These effects are associated with the greater risk of several skin disorders including photoaging and photocarcinogenesis. We have shown that UV-induced infiltrating leukocytes contribute in developing oxidative stress in UV-irradiated skin. The peak period of UV-induced infiltrating leukocytes lies between 48 and 72 h after UV exposure of the skin. In this study we demonstrated that UV (90 mJ/cm2)-induced infiltrating CD11b+ cells in C3H/HeN mice skin were the major source of oxidative stress. Hydrogen peroxide (H2O2) was determined as a marker of oxidative stress. Flow cytometric analysis of viable cells revealed that the number of CD11b+H2O2+ cells were significantly higher (31.8%, P < 0.001) in UV-irradiated skin in comparison with non-UV-exposed skin (0.4%). Intraperitoneal administration of monoclonal antibodies to CD11b (rat IgG2b) to C3H/HeN mice inhibited UVB-induced infiltration of leukocytes, as evidenced by reduction in myeloperoxidase activity (64-80%, P < 0.0005), concomitant with significant reduction in H2O2 production both in epidermis and dermis (66-83%, P < 0.001-0.0005) when compared with the administration of rat IgG2b isotype of anti-CD11b. Furthermore, CD11b+ and CD11b- cell subsets were separated by immunomagnetic cell isolation technique from total epidermal and dermal single cell suspensions obtained 48 h after UV irradiation of the skin and analyzed for H2O2 production. Analytical data revealed that CD11b+ cell population from UV-irradiated skin resulted in significantly higher production of total H2O2 in both epidermis and dermis (87-89%, P < 0.0001) in comparison with CD11b- cell population (11-13% of total H2O2). These data revealed that infiltrating CD11b+ cells were the major source of oxidative stress in UV-irradiated skin and thus may contribute to photoaging and promotion of skin tumor growth within the UV-irradiated skin. Together, these data suggest that reduction in UV-induced skin infiltration of CD11b+ cells may be an alternative and effective strategy to reduce solar UV light-induced oxidative stress-mediated skin disorders including photoaging and photocarcinogenesis.
Computational aberration compensation by coded-aperture-based correction of aberration obtained from optical Fourier coding and blur estimation (2019)

We report a novel generalized optical measurement system and computational approach to determine and correct aberrations in optical systems. The system consists of a computational imaging method capable of reconstructing an optical system's pupil function by adapting overlapped Fourier coding to an incoherent imaging modality. It recovers the high-resolution image latent in an aberrated image via deconvolution. The deconvolution is made robust to noise by using coded apertures to capture images. We term this method coded-aperture-based correction of aberration obtained from overlapped Fourier coding and blur estimation (CACAO-FB). It is well-suited for various imaging scenarios where aberration is present and where providing a spatially coherent illumination is very challenging or impossible. We report the demonstration of CACAO-FB with a variety of samples including an <i>in vivo</i> imaging experiment on the eye of a rhesus macaque to correct for its inherent aberration in the rendered retinal images. CACAO-FB ultimately allows for an aberrated imaging system to achieve diffraction-limited performance over a wide field of view by casting optical design complexity to computational algorithms in post-processing.
How small should pixel size be? (2000)

Pixel design is a key part of image sensor design. After deciding on pixel architecture, a fundamental tradeoff is made to select pixel size. A small pixel size is desirable because it results in a smaller die size and/or higher spatial resolution; a large pixel size is desirable because it results in higher dynamic range and signal-to-noise ratio. Given these two ways to improve image quality and given a set of process and imaging constraints an optimal pixel size exists. It is difficult, however, to analytically determine the optimal pixel size, because the choice depends on many factors, including the sensor parameters, imaging optics and the human perception of image quality. This paper describes a methodology, using a camera simulator and image quality metrics, for determining the optimal pixel size. The methodology is demonstrated for APS implemented in CMOS processes down to 0.18 (mu) technology. For a typical 0.35 (mu) CMOS technology the optimal pixel size is found to be approximately 6.5 micrometers at fill factor of 30%. It is shown that the optimal pixel size scales with technology, btu at slower rate than the technology itself.
Cytotoxic T lymphocyte granules are secretory lysosomes, containing both perforin and granzymes. (1991)

Cytotoxic T lymphocytes (CTL) contain granules that are exocytosed during specific interaction with target cells (TC). In this process, the granule contents, including the lethal protein perforin, as well as granzymes, a family of serine esterases, are delivered to the TC. Information regarding the routing of these proteins towards the granule and their exact localization within the granule is of primary importance to resolve the mechanism of granule-mediated TC killing. In this study, the subcellular localization of perforin, granzymes, and known endosomal and lysosomal marker proteins was determined in human and murine CTL, by immunogold labeling of ultrathin cryosections followed by electron microscopy. Perforin and granzymes can be detected in rough endoplasmic reticulum, Golgi complex, trans-Golgi reticulum, and in all cytotoxic granules. Within the granules, they have a similar distribution and are localized not only in the so-called dense core but also over the region containing small internal vesicles. This finding implies that perforin and granzymes can be released in membrane-enveloped and/or -associated form into the intercellular cleft formed upon CTL-TC interaction. On the basis of the present evidence, additional release of these molecules in soluble form cannot be excluded. The lysosomal membrane glycoproteins lamp-1, lamp-2, and CD63, are abundantly present on the granule-delimiting outer membrane, which becomes incorporated into the CTL plasma membrane during lethal hit delivery. In contrast, the cation-dependent mannose 6-phosphate receptor, known to be present in endosomes and absent from lysosomes, is found only in a minority of the granules. Together with our previous findings that the granules are acidic and connected to the endocytic pathway, these observations define CTL granules as secretory lysosomes.
Dysfunctions of Pokeweed Mitogen-stimulated T and B Lymphocyte Responses Induced by Gammaglobulin Therapy (1981)

Lymphocytes obtained from nonimmuno deficient children treated with commercially available preparations of gammaglobulin failed to proliferate and to mature into plasma cells in vitro after stimulation with pokeweed mitogen. The influence of the treatment on lymphocyte functions varied according to the cell population considered. A T helper cell activity was detected in these patients but only in the cell subset bearing receptors for IgG after irradiation. T lymphocytes exerted a suppressive effect that disappeared after irradiation or incubation at 37 degrees C. The suppressive cells were found among E rosette-forming cells depleted of leukocytes bearing receptors for IgG. Their suppressive effect was expressed only in the presence of normal radioresistant T lymphocytes that did not bear Fc receptors for IgG. Similar dysfunctions could be induced in vitro by incubation of normal T and B lymphocytes with gammaglobulin preparations. Because F(ab)'2 fragments or deaggregated preparations of gammaglobulin failed to activate T suppressor lymphocytes, this activation was likely triggered by attachment of Fc portion of denatured IgG to the corresponding membrane receptor. This activation step was prostaglandin E(2)-dependent, suggesting that activated monocytes were involved in the activation process. B lymphocyte responses appeared directly inhibited by attachment of denatured gammaglobulin on membrane Fc receptor. Our observations suggest that immunological effects of gammaglobulin therapy are not limited to antibody transfer, since it also induces subtle modifications of in vitro pokeweed mitogen-stimulated T and B cell responses. These modifications must be considered in interpreting results obtained in immunodeficient patients investigated under gamma-globulin therapy.
Overexpression of HER2 (erbB2) in Human Breast Epithelial Cells Unmasks Transforming Growth Factor β-induced Cell Motility (2004)

We have examined overexpression of the human epidermal growth factor receptor 2 (HER2) to determine if it modifies the anti-proliferative effect of transforming growth factor (TGF)-beta against MCF-10A human mammary epithelial cells. Exogenous TGF-beta inhibited cell proliferation and induced Smad-dependent transcriptional reporter activity in both MCF-10A/HER2 and MCF-10A/vector control cells. Ligand-induced reporter activity was 7-fold higher in HER2-overexpressing cells. In wound closure and transwell assays, TGF-beta induced motility of HER2-transduced, but not control cells. The HER2-blocking antibody trastuzumab (Herceptin) prevented TGF-beta-induced cell motility. Expression of a constitutively active TGF-beta type I receptor (ALK5(T204D)) induced motility of MCF-10A/HER2 but not MCF-10A/vector cells. TGF-beta-induced motility was blocked by coincubation with either the phosphatidylinositol 3-kinase inhibitor LY294002, the mitogen-activated protein kinase (MAPK) inhibitor U0126, the p38 MAPK inhibitor SB202190, and an integrin beta(1) blocking antibody. Rac1 activity was higher in HER2-overexpressing cells, where both Rac1 and Pak1 proteins were constitutively associated with HER2. Both exogenous TGF-beta and transduction with constitutively active ALK5 enhanced this association. TGF-beta induced actin stress fibers as well as lamellipodia within the leading edge of wounds. Herceptin blocked basal and TGF-beta-stimulated Rac1 activity but did not repress TGF-beta-stimulated transcriptional reporter activity. These data suggest that 1) overexpression of HER2 in nontumorigenic mammary epithelial is permissive for the ability of TGF-beta to induce cell motility and Rac1 activity, and 2) HER2 and TGF-beta signaling cooperate in the induction of cellular events associated with tumor progression.
Observations of standing waves on an inductive plasma coil modeled as a uniform transmission line (1997)

As previously reported by the author at the 47th Annual GEC in paper AA-5 (1994) voltage and current standing waves have been observed along two-and four-turn inductive coils operated at 13.56 MHz. Inductive rf power was used to generate a CHF3 plasma at 4 mTorr. Data from voltage and current probes installed on each end of the coil were used to derive spatial variations of V and I by using the standard formalism of a uniform transmission line. The variation of the effective distributed plasma inductance is presented as well as deviations from transverse electromagnetic modes. The ability to describe the spatial variation of V and I along the coil is useful in the design of inductive plasma etching systems, since both radial and azimuthal variations can potentially affect uniformity of etch and selectivity. The applicability of standard transmission line theory to these coils makes this information readily available via the transmission matrix.
Modern techniques of power spectrum estimation (1967)

The paper discusses the impact of the fast Fourier transform on the spectrum of time series analysis. It is shown that the computationally fastest way to calculate mean lagged products is to begin by calculating all Fourier coefficients with a fast Fourier transform and then to fast-Fourier-retransform a sequence made up of <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">a_{k}^{2}+b_{k}^{2}</tex> (where <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">a_{k}+ib_{k}</tex> are the complex Fourier coefficients). Also discussed are raw and modified Fourier periodograms, bandwidth versus stability aspects, and aims and computational approaches to complex demodulation. Appendixes include a glossary, a review of complex demodulation without fast Fourier transform, and a short explanation of the fast Fourier transform.
Pharmacological evidence for rapid destruction of efferent renal nerves in rats by intrarenal infusion of 6-hydroxydopamine. (1985)

In a previous study we reported that intrarenal (i.r.) infusion of 1 mg 6-hydroxydopamine (6-OHDA) in rats resulted in selective efferent denervation of the infused kidney. Although the vasoconstrictor response to electrical stimulation of the posterior hypothalamus (PH) was already abolished 24 h after infusion, norepinephrine (NE) content of the kidney was reduced by only approximately 50% at that time. In the present study, the status of renal nerves 45 min after infusion of 6-OHDA by i.r. application of scorpion venom (SV), a NE releasing agent, was investigated. In saline pretreated rats, 10 micrograms SV i.r. caused a rapid increase (+355 +/- 80%) in vascular resistance in the injected kidney, whereas only minor changes were observed in resistances of the non-injected kidney, mesentery and hindquarters. Pretreatment of animals with phentolamine (1 mg/kg i.v.) largely abolished the vasoconstrictor response (+57 +/- 20%), confirming dependence of the effect of SV on release of NE from nerve terminals. Finally, pretreatment with 1 mg 6-OHDA i.r. also resulted in abolition of renal vasoconstriction following SV (+7 +/- 6%). The results indicate that as early as 45 min after i.r. infusion of 1 mg 6-OHDA in rats, efferent renal nerve endings are no longer functional.
Cart Auditor: A Compliance and Training Tool for Cashiers at Checkout (2010)

Shopping carts have traditionally been used as a tool provided to the customers in retail stores to carry items from the shelf to checkout stations. These days shopping carts can also be used as a security checkpoint to prevent store losses. All the items collected in a shopping cart are supposed to be unloaded at the checkout station to be scanned and included in the bill. Any items left in the cart intentionally or by accident will not be charged and therefore cause a loss to the store. We propose a system that automatically detects shopping carts and verify their emptiness at the checkout station. We use motion segmentation, line detection, and template matching methods for the cart detection and emptiness verification. An inter-frame edge difference, cart’s path accumulator, and a finite state model are introduced for accurate cart detection. All detected carts are compared with empty cart models and the dissimilarity scores are calculated to verify the emptiness. The proposed system was evaluated on a long video clip (~12 hours) and showed promising results both in cart detection and emptiness verification.
Biometric Recognition in Automated Border Control (2016)

The increasing demand for traveler clearance at international border crossing points (BCPs) has motivated research for finding more efficient solutions. Automated border control (ABC) is emerging as a solution to enhance the convenience of travelers, the throughput of BCPs, and national security. This is the first comprehensive survey on the biometric techniques and systems that enable automatic identity verification in ABC. We survey the biometric literature relevant to identity verification and summarize the best practices and biometric techniques applicable to ABC, relying on real experience collected in the field. Furthermore, we select some of the major biometric issues raised and highlight the open research areas.
Design and evaluation of an integrated Quiet, Clean General Aviation Turbofan (QCGAT) engine and aircraft propulsion system (1980)

The design was based on the LTS-101 engine family for the core engine. A high bypass fan design (BPR=9.4) was incorporated to provide reduced fuel consumption for the design mission. All acoustic and pollutant emissions goals were achieved. A discussion of the preliminary design of a business jet suitable for the developed propulsion system is included. It is concluded that large engine technology can be successfully applied to small turbofans, and noise or pollutant levels need not be constraints for the design of future small general aviation turbofan engines.
Overexpression of anion exchanger 2 in human hepatocellular carcinoma. (2006)

To compare gene expression patterns between the poorly-differentiated (HA22T/VGH) and well-differentiated (HepG2) hepatocellular carcinoma cells, messenger RNA was isolated form both kinds of cells and subjected to differential displays reversing transcriptase-polymerase chain reaction (DDRT-PCR) technology. Gene fragments showing difference in the expression were recovered, reamplified, cloned and sequenced. Anion exchanger 2 (AE2), an isoform of band 3 protein, was identified and chosen for further characterization. AE2 was strongly expressed in HA22T/VGH cells, while it was little expressed in the well-differentiated hepatocellular carcinoma cells (PLC/PRF5 and HepG2) or little in the normal liver cell (Chang liver). In the 28 pairs of HCC and adjacent non-tumor tissues, levels in the cancer tissue (32.7 +/- 5.0) were significantly higher than that in the adjacent non-tumor tissue (9.1 +/- 2.4) (P < 0.01). Moreover, 19 cases (68%) showed over-expression of AE2 in HCC tissues, 3 cases were similar in both tissues, and 6 cases exhibited little or undetectable signals. Twenty cases (71%) of adjacent normal tissue showed little or undetectable signals. The results indicated that overexpression of AE2 may be involved in the development of human HCC.
Uses of ultrasound in audiology. (1990)

Use of diagnostic ultrasound has received little attention in the field of audiology. Recent advances in technology and smaller probe sizes have led to recent strides in development of this procedure for assessment of the middle ear (A and B scan) and evaluation of basilar cerebral arteries (transcranial Doppler). Theory, procedures, and instrumentation are described for evaluation of middle ear structures, determination of middle ear fluid and obtaining precise size and distance measurements. Audiologists are well suited to the study and application of diagnostic ultrasound by virtue of their training in sound propagation, acoustics, functional anatomy, and current involvement in the evaluation of the vestibular system.
New 1-(heterocyclylalkyl)-4-(propionanilido)-4-piperidinyl methyl ester and methylene methyl ether analgesics (1991)

A series of new 1-(heterocyclyalkyl)-4-(propionanilido)-4-piperidinyl methyl esters and methylene methyl ethers have been synthesized and pharmacologically evaluated. In the mouse hot-plate test, the majority of compounds exhibited an analgesia (ED50 less than 1 mg/kg) superior to that of morphine. These studies revealed a pharmacological accommodation for many more structurally diverse and far bulkier aromatic ring systems than the corresponding components of the arylethyl groups of the prototypic methyl ester (carfentanil, 2) and methylene methyl ether (sufentanil, 3 and alfentanil, 4) 4-propionanilido analgesics. Compound 9A (methyl 1-[2-(1H-pyrazol-1-yl)-ethyl]-4-[(1-oxopropyl)phenylamino]-4- piperidinecarboxylate), which exhibited appreciable mu-opioid receptor affinity, was a more potent and short-acting analgesic, than alfentanil with less respiratory depression in the rat. On the other hand, the phthalimides 57A and 57B, which exhibited negligible affinity for opioid receptors associated with the mediation of nociceptive transmission (i.e., mu-, kappa-, and delta-subtypes), displayed analgesic efficacy in all antinociception tests. In addition, while 57B, compared to clinical opioids, showed a superior recovery of motor coordination after regaining of righting reflex from full anesthetic doses in the rat rotorod test, 57A showed significantly less depression of cardiovascular function at supraanalgesic doses in the isoflurane-anesthetized rat.
PML(NLS<sup>-</sup>) Inhibits Cell Apoptosis and Promotes Proliferation in HL-60 Cells (2013)

Promyelocytic leukemia (PML) is a cell-growth suppressor, and PML-retinoic acid receptor α (PML-RARα) is known as a fusion gene of acute promyelocytic leukemia (APL). Studies have reported that neutrophil elastase(NE) cleaved bcr-1-derived PML-RARα in early myeloid cells leading to the removal of nuclear localization signal (NLS) from PML. The resultant PML without NLS named PML(NLS(-)). PML(NLS(-)) mainly locates and functions in the cytoplasm. PML(NLS(-)) may act in different ways from PML, but its biological characteristics have not been reported. In this study, the PML (NLS(-)) was silenced with shRNA [HL-60/pPML(NLS(-))-shRNA] and over-expressed by preparation of recombinant adenovirus [HL-60/pAd-PML(NLS(-))]. The mRNA and protein expression of PML(NLS(-)) were detected by RT-PCR and Western blot respectively. Cell proliferation in vitro was assessed by MTT assay. Flow cytometry (FCM) was used to detect apoptotic cells. The transcription of BCL-2, BAX and C-MYC was detected in HL-60/pAd-PML(NLS(-)) cells. Our results showed that compared to the control group, the expression of PML(NLS(-)) was significantly reduced in the HL-60/pPML(NLS(-))-shRNA cells, and increased significantly in the HL-60/pAd-PML(NLS(-)) cells. The proliferation was significantly inhibited in the HL-60/pPML(NLS(-))-shRNA cells in a time-dependent manner, but markedly promoted in the HL-60/pAd-PML(NLS(-)) cells treated with 60 μmol/L emodin. FCM revealed the apoptosis increased in HL-60/pPML(NLS(-))-shRNA cells, and decreased in the HL-60/pAd-PML(NLS(-)) cells. The expression of BAX decreased significantly, while that of BCL-2 and C-MYC increased significantly in HL-60/ pAd-PML(NLS(-)) cells. Down-regulation of PML(NLS(-)) expression inhibits the proliferation and induces the apoptosis of HL-60 cells. On the contrary, over-expression of PML(NLS(-)) promotes the proliferation and reduce the emodin-induced apoptosis of HL-60 cells.
Separation of Metallic from Semiconducting Single‐Walled Carbon Nanotubes. (2003)

Abstract For Abstract see ChemInform Abstract in Full Text.
Multimedia service interworking over heterogeneous networking environments (1999)

This article discusses technical issues related to delivery of multimedia services, such as video-on-demand, over heterogeneous networking environments. In particular, it describes the design and implementation of an experimental system for multimedia service interworking between a DAVIC (Digital Audio Visual Council) domain and an Internet domain. The system, called MIDDLEMEN (middlemen for multimedia environment), consists of the broker and the interworking unit, which work together to provide multimedia services across different types of networks in a seamless manner. The broker acts as a guide to multimedia services, and complements servers or clients that are not equipped with full functionality. The broker also controls the resources in MIDDLEMEN. The interworking unit performs various translation functions under the control of the broker, including stream transport protocol conversion, traffic monitoring and bit-rate control, service gateway conversion, and stream control conversion. Current implementation supports the delivery of a DAVIC-compliant VOD service from an ATM network to an IP network.