gebhart/patrepeval huggingface dataset:
A parser for real-time speech synthesis of conversational texts (1992)

In this paper, we concern ourselves with an application of text-to-speech for speech-impaired, deaf, and hard of hearing people. The application is unusual because it requires real-time synthesis of unedited, spontaneously generated conversational texts transmitted via a Telecommunications Device for the Deaf (TDD). We describe a parser that we have implemented as a front end for a version of the Bell Laboratories text-to-speech synthesizer (Olive and Liberman 1985). The parser prepares TDD texts for synthesis by (a) performing lexical regularization of abbreviations and some non-standard forms, and (b) identifying prosodic phrase boundaries. Rules for identifying phrase boundaries are derived from the prosodic phrase grammar described in Bachenko and Fitzpatrick (1990). Following the parent analysis, these rules use a mix of syntactic and phonological factors to identify phrase boundaries but, unlike the parent system, they forgo building any hierarchical structure in order to bypass the need for a stacking mechanism; this permits the system to operate in near real time. As a component of the text-to-speech system, the parser has undergone rigorous testing during a successful three-month field trial at an AT&T telecommunications center in California. In addition, laboratory evaluations indicate that the parser's performance compares favorably with human judgments about phrasing.
The SINS database for detection of daily activities in a home environment using an Acoustic Sensor Network (2017)

There is a rising interest in monitoring and improving human well-being at home using different types of sensors including microphones. In the context of Ambient Assisted Living (AAL) person’s are monitored, e.g. to support patients with a chronic illness and older persons, by tracking their activities being performed at home. When considering an acoustic sensing modality, a performed activity can be seen as an acoustic scene. Recently, acoustic detection and classification of scenes and events has gained interest in the scientific community and led to numerous public databases for a wide range of applications. However, no public databases exist which a) focus on daily activities in a home environment, b) contain activities being performed in a spontaneous manner, c) make use of an acoustic sensor network, and d) are recorded as a continuous stream. In this paper we introduce a database recorded in one living home, over a period of one week. The recording setup is an acoustic sensor network containing thirteen sensor nodes, with four low-cost microphones each, distributed over five rooms. Annotation is available on an activity level. In this paper we present the recording and annotation procedure, the database content and a discussion on a baseline detection benchmark. The baseline consists of Mel-Frequency Cepstral Coefficients, Support Vector Machine and a majority vote late-fusion scheme. The database is publicly released to provide a common ground for future research.
Synthesis and Histone Deacetylase Inhibitory Activity of Largazole Analogs: Alteration of the Zinc-Binding Domain and Macrocyclic Scaffold (2009)

Fourteen analogs of the marine natural product largazole have been prepared and assayed against histone deacetylases (HDACs) 1, 2, 3, and 6. Olefin cross-metathesis was used to efficiently access six variants of the side-chain zinc-binding domain, while adaptation of our previously reported modular synthesis allowed probing of the macrocyclic cap group.
Surfactantless Synthesis of Silver Nanoplates and Their Application in SERS (2007)

Silver nanoplates with thicknesses of 50-70 nm and edge lengths ranging from 200 nm to 1 mum have been grown on semiconductor wafers at room temperature through a simple galvanic reaction between an aqueous solution of AgNO(3) and n-type GaAs. The as-grown Ag structures have chemically clean surfaces due to no surfactant or coordinating molecules being involved in the synthesis. Electron microscopy characterizations indicate that each Ag plate has rough surfaces and a half-moon morphology with one straight edge and one arclike edge. Systematic studies on varying reaction conditions reveal that the oxide (i.e., Ga(2)O(3) and As(2)O(3)) layers of GaAs, generated in situ in the reactions, play an important role in assisting the growth of anisotropic nanoplates. The cleanliness of the surfaces of the Ag nanoplates is beneficial to attachment of interesting molecules on their surfaces for various applications, such as plasmonic-enhanced photophysical and photochemical processes and surface-enhanced spectroscopies.
Flow cytometric quantification of human chromosome specific repetitive DNA sequences by single and bicolor fluorescent in situ hybridization to lymphocyte interphase nuclei (1990)

Fluorescent in situ hybridization allows for rapid and precise detection of specific nucleic acid sequences in interphase and metaphase cells. We applied fluorescent in situ hybridization to human lymphocyte interphase nuclei in suspension to determine differences in amounts of chromosome specific target sequences amongst individuals by dual beam flow cytometry. Biotinylated chromosome 1 and Y specific repetitive satellite DNA probes were used to measure chromosome 1 and Y polymorphism amongst eight healthy volunteers. The Y probe fluorescence was found to vary considerably in male volunteers (mean fluorescence 169, S.D. 35.6). It was also detectable in female volunteers (mean fluorescence 81, S.D. 10.7), because 5-10% of this repetitive sequence is located on autosomes. The Y probe fluorescence in males was correlated with the position of the Y chromosome cluster in bivariate flow karyotypes. When chromosome 1 polymorphism was studied, one person out of the group of eight appeared to be highly polymorphic, with a probe fluorescence 26% below the average. By means of fluorescent in situ hybridization on a glass slide and bivariate flow karyotyping, this 26% difference was found to be caused by a reduction of the centromere associated satellite DNA on one of the homologues of chromosome 1. The simultaneous hybridization to human lymphocyte interphase nuclei of biotinylated chromosome 1 specific repetitive DNA plus AAF-modified chromosome Y specific DNA was detected by triple beam flow cytometry. The bicolor double hybridized nuclei could be easily distinguished from the controls. When the sensitivity of this bicolor hybridization is improved, this approach could be useful for automatic detection of numerical chromosome aberrations, using one of the two probes as an internal control.
Structure of Eukaryotic RNA Polymerases (2008)

The eukaryotic RNA polymerases Pol I, Pol II, and Pol III are the central multiprotein machines that synthesize ribosomal, messenger, and transfer RNA, respectively. Here we provide a catalog of available structural information for these three enzymes. Most structural data have been accumulated for Pol II and its functional complexes. These studies have provided insights into many aspects of the transcription mechanism, including initiation at promoter DNA, elongation of the mRNA chain, tunability of the polymerase active site, which supports RNA synthesis and cleavage, and the response of Pol II to DNA lesions. Detailed structural studies of Pol I and Pol III were reported recently and showed that the active center region and core enzymes are similar to Pol II and that strong structural differences on the surfaces account for gene class-specific functions.
Multithreaded systolic/SIMD DSP array processor-MUS2DAP (2002)

This paper deals with architectural design of the systolic/SIMD DSP processing array called MUS2DAP optimized for the execution of DSP filtering, vector/matrix and linear algebra algorithms. Its main features are architectural support for multithreading, interprocessor communication based on virtual channels and exploitation of instruction level parallelism within each processing cell of the array. Up to four threads can be executed in an interleaved fashion on one processor array. Physical implementation details of the processor network topology are hidden from programmer, since instructions used for interprocessor communication operate on virtual channels. The same algorithm can thus be executed unchanged on multiple physically implemented interconnection network topologies ranging from linear, 2D mesh to hex array. Architecture is optimized to execute algorithms in single precision 32-bit floating-point arithmetic. There are two controllers available, each controlling part of the processing array. This feature is helpful in implementing linear algebra algorithms which usually have processing cells with different functionalities within single array.
Role of Group I Metabotropic Glutamate Receptors in the Patterning of Epileptiform Activities In Vitro (1997)

In guinea pig hippocampal slices, picrotoxin elicited spontaneous epileptiform bursts 300-550 ms in duration. Additional application of (R,S)-3,5-dihydroxyphenylglycine or (S)-3-hydroxyphenylglycine, agonists specific for group I metabotropic glutamate receptors (mGluRs), or (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid, a broad-spectrum mGluR agonist, converted picrotoxin-induced interictal bursts into prolonged discharges measured on the order of seconds. The prolonged discharges induced by selective group I mGluR agonist continued to be produced for hours after agonist removal. The antagonists (S)-4-carboxyphenylglycine and (+)-alpha-methyl-4-carboxyphenylglycine had no effect on the duration of picrotoxin-induced interictal bursts. However, after agonist exposure, the persistent prolonged discharges occurring in the absence of agonist were reversibly suppressed by the antagonists, suggesting that the activity is maintained via endogenous activation of group I mGluRs by synaptically released glutamate. Our results suggest that, under some conditions, activation of group I mGluRs produces long-lasting enhancement of synaptic responses, mediated at least in part by autopotentiation of the group I mGluR response itself, which may result in the production of seizure discharges and contribute to epileptogenesis.
Neuroinflammation‐induced acceleration of amyloid deposition in the APP<sup>V717F</sup> transgenic mouse (2001)

It has been postulated that neuroinflammation plays a critical role in the pathogenesis of Alzheimer's disease (AD). To directly test whether an inflammatory stimulus can accelerate amyloid deposition in vivo, we chronically administered the bacterial endotoxin, lipopolysaccharide (LPS), intracerebroventricularly (i.c.v.) to 2-month-old APPV717F+/+ transgenic (TG) mice, which overexpress a mutant human amyloid precursor protein (APP 717V-F) with or without apolipoprotein E (apoE) for 2 weeks. Two weeks following central LPS administration a striking global reactive astrocytosis with increased GFAP immunoreactivity was found throughout the brains of all LPS-treated wild-type and transgenic mice including the contralateral brain hemisphere. Localized microglial activation was also evident from lectin immunostaining adjacent to the cannula track of LPS-treated mice. Quantification of thioflavine-S-positive Abeta deposits revealed a marked acceleration of amyloid deposition in LPS-treated APPV717F+/+-apoE+/+ mice compared to nontreated or vehicle-treated APPV717F+/+-apoE+/+ mice (P = 0.005). By contrast, no amyloid deposits were detected by thioflavine-S staining in LPS or vehicle-treated apoE-deficient APPV717F TG mice. Our data suggest that neuroinflammation can accelerate amyloid deposition in the APPV717F+/+ mouse model of AD and that this process requires the expression of apoE.
Synthesis of protein-coated gelatin microspheres and their use as microcarriers for cell culture. Part I. Derivatization with native collagen (1991)

A novel technique for the synthesis of native collagen (type I)-coated gelatin microspheres was developed using the emulsion-polymerization principle, which is realized in four steps: emulsification, separation, stabilization, and protein modification. A 20% gelatin solution was emulsified in sunflower oil and the resulting beads were polymerized with glutaraldehyde. The novelty of our technique consists in the protein modification step, where we derivatized the beads by saturation of the free aldehyde groups on the cross-linked gelatin in the native collagen solution (0.3 mg/ml in 0.05 M Tris, pH 8.6) and further stabilized the beads with sodium borohydride. The resulting microspheres exhibited excellent properties as microcarriers for in vitro cell culturing using Vero cells as the model system. In comparison with pure gelatin beads, the cells attached more rapidly and grew faster on native collagen-coated beads. Approximately 90% of the Vero cells attached to the microcarrier after 1 h. After a lag period of nearly 24 h, the cells began to grow rapidly and reached confluence for 4-5 days, whereas the cells on pure gelatin beads reached the same density about 1 or 2 days later.
Evaluation of Microencapsulated Islets in Agarose Gel as Bioartificial Pancreas by Studies of Hormone Secretion in Culture and by Xenotransplantation (1989)

Islets were microencapsulated in agarose gel for examination of the possible use of microencapsulated islets as a bioartificial pancreas. Microencapsulated islets secreted insulin into the culture medium (RPMI-1640) and could rapidly increase their insulin release in response to a glucose challenge even after greater than 100 days. Hamster islets in groups of 400-1000 encapsulated in microbeads containing 11-14% (wt/wt) agarose were xenogenically transplanted into the peritoneal cavity of five diabetic mice. The longest normoglycemic period in these mice was 53 days, which was markedly longer than the normoglycemic period obtained by nonencapsulated islets. Agarose seems to be a suitable basic material for encapsulating islets, because the islets can easily be microencapsulated without any adverse effect on the islet function.
Hepatitis B Virus Containing the I233V Mutation in the Polymerase Reverse‐Transcriptase Domain Remains Sensitive to Inhibition by Adefovir (2007)

An isoleucine-to-valine change at position 233 (rtI233V) of hepatitis B virus (HBV) polymerase was recently reported to cause decreased in vitro susceptibility to, and treatment failure of, adefovir dipivoxil (ADV). To further evaluate these findings, we screened our ADV clinical-study sequence database of 853 patients and identified 4 who, at baseline, had HBV with this mutation. All 4 patients responded to treatment with ADV, with a median change in HBV DNA levels of 4.0 log(10) copies/mL after 48 weeks of treatment. Phenotypic evaluation of clinical isolates and of a laboratory strain with the rtI233V mutation demonstrated their full susceptibility to adefovir in vitro, and HBV with the rtI233V mutation developed in none of the patients.
SAIRE---a scalable agent-based information retrieval engine (1997)

Article Free Access Share on SAIRE—a scalable agent-based information retrieval engine Authors: Jidé B. Odubiyi Lockheed Martin Space Mission systems, Seabrook, MD Lockheed Martin Space Mission systems, Seabrook, MDView Profile , David J. Kocur Lockheed Martin Space Mission systems, Seabrook, MD Lockheed Martin Space Mission systems, Seabrook, MDView Profile , Stuart M. Weinstein Lockheed Martin Space Mission systems, Seabrook, MD Lockheed Martin Space Mission systems, Seabrook, MDView Profile , Nagi Wakim Bowie State University, Bowie, MD Bowie State University, Bowie, MDView Profile , Sadanand Srivastava Bowie State University, Bowie, MD Bowie State University, Bowie, MDView Profile , Chris Gokey Bowie State University, Bowie, MD Bowie State University, Bowie, MDView Profile , JoAnna Graham Bowie State University, Bowie, MD Bowie State University, Bowie, MDView Profile Authors Info & Claims AGENTS '97: Proceedings of the first international conference on Autonomous agentsFebruary 1997Pages 292–299https://doi.org/10.1145/267658.267731Published:08 February 1997Publication History 20citation459DownloadsMetricsTotal Citations20Total Downloads459Last 12 Months45Last 6 weeks12 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Antibody−Dendrimer Conjugates: The Number, Not the Size of the Dendrimers, Determines the Immunoreactivity (2008)

Radioimmunotherapy using antibodies with favorable tumor targeting properties and high binding affinity is increasingly applied in cancer therapy. The potential of this valuable cancer treatment modality could be further improved by increasing the specific activity of the labeled proteins. This can be done either by coupling a large number of chelators which leads to a decreased immunoreactivity or by conjugating a small number of multimeric chelators. In order to systematically investigate the influence of conjugations on immunoreactivity with respect to size and number of the conjugates, the anti-EGFR antibody hMAb425 was reacted with PAMAM dendrimers of different size containing up to 128 chelating agents per conjugation site. An improved dendrimer synthesis protocol was established to obtain compounds of high homogeneity suitable for the formation of defined protein conjugates. The quantitative derivatization of the PAMAM dendrimers with DOTA moieties and the characterization of the products by isotopic dilution titration using (111)In/(nat)In are shown. The DOTA-containing dendrimers were conjugated with high efficiency to hMAb425 by applying Sulfo-SMCC as cross-linking agent and a 10- to 25-fold excess of the thiol-containing dendrimers. The determination of the immunoreactivities of the antibody-dendrimer conjugates by FACS analysis revealed a median retained immunoreactivity of 62.3% for 1.7 derivatization sites per antibody molecule, 55.4% for 2.8, 27.9% for 5.3, and 17.1% for 10.0 derivatization sites per antibody but no significant differences in immunoreactivity for different dendrimer sizes. These results show that the dendrimer size does not influence the immunoreactivity of the derivatized antibody significantly over a wide molecular weight range, whereas the number of derivatization sites has a crucial effect.
150% magnetoresistance in sputtered Fe/Cr(100) superlattices (1993)

We report the epitaxial growth of Fe/Cr(100) superlattices onto MgO(100) single-crystal substrates by magnetron sputtering. Superlattices that are epitaxially oriented within 1° both in-plane and out-of-plane with the MgO substrate are achieved by initial growth of a Cr base layer at high temperature. Multiple superlattice diffraction peaks are observed in the low- and high-angle x-ray diffraction spectra. Three peaks are observed in the magnetoresistance associated with the oscillatory antiferromagnetic interlayer magnetic coupling as a function of Cr thickness. A maximum magnetoresistance of 150% at 4.2 K (28% at room temperature) is observed for a Cr(100 Å)/[Fe(14 Å)/Cr(8 Å)]50 superlattice.
Synthesis of allyloxycarbonyloxymethyl-5-fluorouracil and copolymerizations with N-vinylpyrrolidinone (2000)

Poly(N-vinylpyrrolidinone) (PNVP) bearing 5-fluorouracil (5-FU) moieties was synthesised via a three-step method. Firstly, 5-FU reacted with formaldehyde to form a mixture of mono- and disubstituted hydroxymethyl-5-FUs. The mixture was then treated with allyl chloroformate to afford allyloxycarbonyloxymethyl-5-FUs. This reaction showed site-specificity: the hydroxymethyl goup at the N-1 position readily reacted with chloroformate whereas the N-3 hydroxymethyl group partially decomposed into formaldehyde and the amide group. 1-Allyloxycarbonyloxymethyl-5-FU (4) and NVP were copolymerized in dioxane using azobisisobutyronitrile as an initiator. The monomer reactivity ratios, r4 = 0.32 and rNVP = 0.97, were evaluated by both linear and non-linear methods.
Inhibition of leukocyte candidacidal activity by serum from patients with disseminated candidiasis. (1975)

The effect of normal serum and serum for seven patients with disseminated candidiasis (DC) on candidacidal activity of normal human neutrophils (PMNL) was studied. PMNL incubated in DC serum had depressed candiacidal activity when compared to studies with normal serum. The candidacidal defect was not related to differences in particle uptake since phagocytic indices were similar with either normal or DC serum. DC serum did not affect PMNL bactericidal activity against Staphylococcus aureus. When Candida albicans (CA) was incubated in either normal or DC sera, washed, and added to PMNL in normal serum, pronounced inhibition of candidacidal activity was seen in all studies with CA previously incubated in DC serum. Dilution of DC serum or absorption of DC serum with heat-killed CA reversed the candidacidal defect. Ultrastructural studies of phagocytized CA demonstrated small ("tight") phagolysosomes and a delay in breakdown of an electron-dense coating on CA previously incubated in DC serum when compared to controls. Inhibition of leukocyte candidacidal activity appeared to be related to high titers of IgG antibodies against CA present in DC serum.
Preparation and Evaluation of Carborane Analogues of Tamoxifen (2010)

A stereoselective synthesis of closo carborane analogues of tamoxifen was developed where the products represent a new approach to developing metabolically robust SERMs. The A-ring found in the backbone of tamoxifen was replaced with an ortho carborane cluster; the product was determined to be the desired Z isomer, which showed superior chemical stability to tamoxifen both in solution and in the solid state. By use of microwave heating, it was possible to convert some of the Z carborane tamoxifen analogue to the corresponding E isomer. Cell growth assays using both isomers and a carborane that is known to target the ER were conducted using estrogen receptor (ER) positive and ER negative human breast cancer cells with and without the presence of estradiol (E2). The Z carborane isomer was able to inhibit cell proliferation better than tamoxifen in an E2 free environment, while the E isomer inhibited cell growth better than tamoxifen when E2 was present.
Site-specific metastasis formation (2009)

The metastatic spread of tumors is a well-coordinated process in which different types of cancers tend to form metastases in defined organs. The formation of site-specific metastases requires full compatibility between the intrinsic properties of the tumor cells and the tumor microenvironment. It was recently found that chemokines which are expressed in specific loci promote the adhesion, migration and invasion of tumor cells that express the corresponding receptor(s). Of the different members of the family, the CXCL12 chemokine and its cognate CXCR4 receptor are the prototypes of this process, although other members of the family (e.g. CCR7 and CCR10) also play a role in determination of the metastatic spread. This commentary addresses the fundamental roles of chemokines and their receptors in site-specific metastasis, with emphasis on CXCL12-CXCR4. The article also describes some of the efforts that were performed thus far in order to identify the intracellular components involved in this process. The focus is put on the roles played by proteins that regulate adhesion and migration of tumor cells in response to CXCL12, including mainly focal adhesion kinase (FAK), Pyk2/RAFTK and members of the Rho family of GTPases (RhoA, Rac, Cdc42). This is followed by discussion of open questions that need to be addressed in future research, and of the potential therapeutic implications of the findings that are available to date in this field.