gebhart/patrepeval huggingface dataset:
Intraarticular Gene Transfer of TNFR:Fc Suppresses Experimental Arthritis with Reduced Systemic Distribution of the Gene Product (2002)

Tumor necrosis factor alpha (TNFalpha) plays a pivotal role in the pathogenesis of rheumatoid arthritis (RA). Blockage of TNFalpha actions by systemic administration of TNF antagonists has recently been shown to ameliorate joint symptoms in RA patients. In the present study, a streptococcal cell wall (SCW)-induced rat arthritis model was used to evaluate the effect of different gene transfer routes of a TNF antagonist on the development and severity of arthritis. Successful delivery of a plasmid DNA encoding a rat TNF receptor-immunoglobulin Fc (TNFR:Fc) fusion gene prompted the subsequent administration of a recombinant adeno-associated virus (rAAV) vector encoding the antagonist, either locally (intraarticular) or systemically (intramuscular). The TNFR:Fc gene, delivered by either route, resulted in profound suppression of the arthritis as reflected in decreased inflammatory cell infiltration, pannus formation, cartilage and bone destruction, and mRNA expression of joint proinflammatory cytokines. Increased bioactive serum TNFR levels were detected as a result of rAAV-ratTNFR:Fc administration, concomitant with a decrease in circulating TNFalpha. Administration of the rAAV-ratTNFR:Fc vector to one joint also suppressed arthritis in the contralateral joint. Importantly, intraarticular administration resulted in significantly lower systemic distribution of the gene product. Hence, the use of rAAV as the delivery vector for TNFR:Fc effectively suppressed SCW-induced arthritis and may provide an approach for local delivery of antiarthritic therapy.
Glycosylation of Natural Human Neutrophil Gelatinase B and Neutrophil Gelatinase B-Associated Lipocalin (1999)

Gelatinase B is a matrix metalloproteinase (MMP-9) involved in tissue remodeling, development, cancer, and inflammation. Neutrophils produce three major forms of (pro)gelatinase B: 92 kDa monomers, homodimers, and complexes of gelatinase B covalently bound to neutrophil gelatinase B-associated lipocalin (NGAL). In contrast to the case for other proteinases, little information about the glycosylation of any natural human MMP is available. Here, both gelatinase B and NGAL were purified from human peripheral blood neutrophils, and the entire contents of the released N- and O-glycan pools were analyzed simultaneously using recently developed high-performance liquid chromatography-based technology. The results are discussed within the context of the domain structure of gelatinase B and a molecular model of NGAL based on data from this study and the three-dimensional nuclear magnetic resonance (NMR) structure of the protein. More than 95% of the N-linked glycans attached to both gelatinase B and NGAL were partially sialylated, core-fucosylated biantennary structures with and without outer arm fucose. The O-linked glycans, which were estimated to comprise approximately 85% of the total sugars on gelatinase B, mainly consisted of type 2 cores with Galbeta1,4GlcNAc (lactosamine) extensions, with or without sialic acid or outer arm fucose. This paper also contains the first report of O-linked glycans attached to NGAL. Although both proteins were isolated from neutrophils and contained O-linked glycans mainly with type 2 cores, the glycans attached to individual serine/threonine residue(s) in NGAL were significantly smaller than those on gelatinase B. In contrast to NGAL, gelatinase B contains a region rich in Ser, Thr, and Pro typical of O-glycosylated mucin-like domains.
Preferential involvement of CX chemokine receptor 4 and CX chemokine ligand 12 in T-Cell migration toward melanoma cells (2006)

Our previous analysis of the role of chemokines in T lymphocyte trafficking toward human tumor cells revealed the migration of a melanoma patient's cytotoxic T lymphocytes (CTL) toward autologous tumor cells, resulting in tumor cell apoptosis, in an organotypic melanoma culture. CTL migration was mediated by CX chemokine receptor (CXCR) 4 expressed by the CTL and CX chemokine ligand (CXCL) 12 secreted by the tumor cells, as evidenced by blockage of CTL migration by antibodies to CXCL12 or CXCR4, high concentrations of CXCL12 or small molecule CXCR4 antagonist. Here, we present the results of T cell migration in one additional melanoma patient and T cell and tumor cell analyses for CXCR4 and CXCL12 expression, respectively, in 12 additional melanoma patients, indicating the preferential role of CXCR4 and CXCL12 in CTL migration toward melanoma cells. These studies add to the increasing body of evidence suggesting that CXCL12 is a potent chemoattractant for T cells.
Biphasic mixture of water, dimethyl sulfoxide, and tetrahydrofuran for use in centrifugal partition chromatography (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBiphasic mixture of water, dimethyl sulfoxide, and tetrahydrofuran for use in centrifugal partition chromatographyA. P. Foucault, P. Durand, E. Camacho. Frias, and F. Le GofficCite this: Anal. Chem. 1993, 65, 15, 2150–2154Publication Date (Print):August 1, 1993Publication History Published online1 May 2002Published inissue 1 August 1993https://pubs.acs.org/doi/10.1021/ac00063a038https://doi.org/10.1021/ac00063a038research-articleACS PublicationsRequest reuse permissionsArticle Views331Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Differential effects of staphylococcal toxic shock syndrome toxin-1 on B cell apoptosis. (1996)

Superantigens, such as toxic shock syndrome toxin 1 (TSST-1), have been implicated in the pathogenesis of several autoimmune and allergic diseases associated with polyclonal B cell activation. In this report, we studied the in vitro effects of TSST-1 on B cell activation. We show herein that TSST-1 produced antagonistic effects on Ig synthesis by peripheral blood mononuclear cells (PBMC) from normal subjects, depending on the concentration used; Ig production was inhibited at 1000 pg/ml (P < 0.01) and enhanced at 1 and 0.01 pg/ml (P < 0.01) of toxin. Cultures of PBMC were then examined for morphologic features and DNA fragmentation characteristic for apoptosis. B cells exhibited a significantly higher (P < 0.01) incidence of apoptosis after stimulation with 1000 pg/ml of TSST-1 compared with 1 or 0.01 pg/ml of toxin or medium alone. Abundant expression of Fas, a cell surface protein that mediates apoptosis, was detected on B cells after stimulation with 1000 pg/ml of TSST-1 and was significantly higher on B cells undergoing apoptosis than on live cells (P = 0.01). Additionally, increased Fas expression and B cell death occurred at concentrations of TSST-1 inducing the production of high amounts of gamma interferon (IFN-gamma), and both events could be blocked by neutralizing anti-IFN-gamma antibody. These findings suggest that high concentrations of TSST-1 can induce IFN-gamma-dependent B cell apoptosis, whereas at low concentrations it stimulates Ig synthesis by PBMC from normal subjects. These findings support the concept that staphylococcal toxins have a role in B cell hyperactivity in autoimmunity and allergy.
Effects of inhaled CO<sub>2</sub>and added dead space on idiopathic central sleep apnea (1997)

We hypothesized that reductions in arterial PCO2 (PaCO2) below the apnea threshold play a key role in the pathogenesis of idiopathic central sleep apnea syndrome (ICSAS). If so, we reasoned that raising PaCO2 would abolish apneas in these patients. Accordingly, patients with ICSAS were studied overnight on four occasions during which the fraction of end-tidal CO2 and transcutaneous PCO2 were measured: during room air breathing (N1), alternating room air and CO2 breathing (N2), CO2 breathing all night (N3), and addition of dead space via a face mask all night (N4). Central apneas were invariably preceded by reductions in fraction of end-tidal CO2. Both administration of a CO2-enriched gas mixture and addition of dead space induced 1- to 3-Torr increases in transcutaneous PCO2, which virtually eliminated apneas and hypopneas; they decreased from 43.7 +/- 7.3 apneas and hypopneas/h on N1 to 5.8 +/- 0.9 apneas and hypopneas/h during N3 (P < 0.005), from 43.8 +/- 6.9 apneas and hypopneas/h during room air breathing to 5.9 +/- 2.5 apneas and hypopneas/h of sleep during CO2 inhalation during N2 (P < 0.01), and to 11.6% of the room air level while the patients were breathing through added dead space during N4 (P < 0.005). Because raising PaCO2 through two different means virtually eliminated central sleep apneas, we conclude that central apneas during sleep in ICSA are due to reductions in PaCO2 below the apnea threshold.
Microchannel heat sinks (1988)

Microchannel heat sinks useful in the cooling of diode laser arrays have been fabricated from InP and exhibit a thermal resistance as low as 0.072 C/(W/sq cm), corresponding to the dissipation of heat loads in excess of 1 kW/sq cm and representing a two-orders-of-magnitude reduction of levels achievable by current methods. The pumping power required to force liquid coolants through microchannel heat sinks can be kept as low as as 10 W/sq cm. Attention is presently given to a thermal- and fluid-performance model for these heat sinks, as well as to illustrative examples of microchannel fabrication for both InP and aluminum. 19 references.
New formulation of the Fourier modal method for crossed surface-relief gratings (1997)

A new formulation of the Fourier modal method (FMM) that applies the correct rules of Fourier factorization for crossed surface-relief gratings is presented. The new formulation adopts a general nonrectangular Cartesian coordinate system, which gives the FMM greater generality and in some cases the ability to save computer memory and computation time. By numerical examples, the new FMM is shown to converge much faster than the old FMM. In particular, the FMM is used to produce well-converged numerical results for metallic crossed gratings. In addition, two matrix truncation schemes, the parallelogramic truncation and a new circular truncation, are considered. Numerical experiments show that the former is superior.
Experimental thermal resistance evaluation of a three-dimensional (3D) chip stack, including the transient measurements (2012)

For the thermal management of three-dimensional (3D) chip stack, its thermal resistance needs to be clearly understood. In this study, 3D stacked test chips are fabricated, which are implemented with PN junction diodes for temperature sensors and diffused resistors for heating. At SemiTherm2011, the equivalent thermal conductivity of the interconnection, including BEOL (Back-End-Of-the-Line, wiring layer) is experimentally obtained to be 1.6W/mC and this time, we measure the thermal effect of Cu TSVs and it is experimentally supported that as the Cu TSV area ratio increases, the thermal conductivity of chip with TSVs in the vertical direction increases, on the contrary, that in the horizontal direction decreases. Also, the transient thermal measurement is performed and its result is compared with steady state measurement result. Further, the thermal capacitance measurement of 3D stacked test chip with hot spot heating is performed, which is essential to determine the transient thermal performance of 3D chip stack.
Natural immune response to the C-terminal 19-kilodalton domain of Plasmodium falciparum merozoite surface protein 1 (1996)

We have characterized the natural immune responses to the 19-kDa domain of merozoite surface protein 1 in individuals from an area of western Kenya in which malaria is holoendemic. We used the three known natural variant forms of the yeast-expressed recombinant 19-kDa fragment that are referred to as the E-KNG, Q-KNG, and E-TSR antigens. T-cell proliferative responses in individuals older than 15 years and the profile of immunoglobulin G (IgG) antibody isotypes in individuals from 2 to 74 years old were determined. Positive proliferative responses to the Q-KNG antigen were observed for 54% of the individuals, and 37 and 35% of the individuals responded to the E-KNG and E-TSR constructs, respectively. Considerable heterogeneity in the T-cell proliferative responses to these three variant antigens was observed in different individuals, suggesting that the 19-kDa antigen may contain variant-specific T epitopes. Among responses of the different isotypes of the IgG antibody, IgG1 and IgG3 isotype responses were predominant, and the prevalence and levels of the responses increased with age. We also found that a higher level of IgG1 antibody response correlated with lower parasite density among young age groups, suggesting that IgG1 antibody response may play a role in protection against malaria. However, there was no correlation between the IgG3 antibody level and protection. Furthermore, we observed that although the natural antibodies cross-reacted with all three variant 19-kDa antigens, IgG3 antibodies in 12 plasma samples recognized only the E-KNG and Q-KNG constructs and not the E-TSR antigen. This result suggests that the fine specificity of IgG3 antibodies differentiates among variant-specific natural B-cell determinants in the second epidermal growth factor domain (KNG and TSR) of the antigen.
Identification of a vitamin D-responsive protein on the surface of human osteosarcoma cells. (1989)

Monoclonal antibodies were elicited to membrane constituents of the osteoblastic human osteosarcoma cell line Saos-2. Two types of antibody reactivities were characterized: one group of antibodies identified fibroblastic and osteoblastic cultured cells, whereas the other group was specific for the parent cell line, Saos-2. Primary endothelial cells and hepatoma cells were not recognized by either group of antibodies. Through indirect immunofluorescent microscopy, the Saos-2-specific antigen was demonstrated to reside on the surface of these osteosarcoma cells. Metabolic radiolabeling of cultured Saos-2 cells and subsequent immunoprecipitation, electrophoretic separation, and autoradiography revealed this protein to have a Mr of 80,000. Similar experiments in the presence of hormones showed that the expression of this cell surface protein was influenced in an opposing fashion by the bone-regulating hormones parathyroid hormone and vitamin D. Vitamin D stimulated expression by 300%, whereas parathyroid hormone depressed expression by 50%. Thus, Saos-2 human osteoblastic cells demonstrate hormonal regulation through an apparently specific membrane protein.
Hypothermia Therapy for Brain Injury (2009)

Induced hypothermia is an acknowledged useful therapy for treating conditions that lead to cell and tissue damage caused by ischemia, including traumatic brain injury, stroke, and cardiac arrest. An accumulating body of clinical evidence, together with several decades of research, has documented that the efficacy of hypothermia is dependent on achieving a reduced temperature in the target tissue before or soon following the ischemia-precipitating event. The temperature must be lowered to within a rather small range of values to effect therapeutic benefit without introducing collateral problems. Rewarming must be much slower than cooling. Many different methods and devices have been used for cooling, with mixed results. There are existing opportunities for bioengineers to improve our understanding of the mechanisms of hypothermia and to develop more effective methods of cooling the brain following trauma.
The All-Electric Fighter Airplane Flight Control Issues, Capabilities, and Projections (1984)

The prime issues raised in any all-electric airplane discussion are (1) is the electric power system as reliable and trustworthy as a hydraulic power system; (2) can electromechanical flight control actuators perform satisfactorily, i.e., can the performance match that of hydraulic actuators; (3) can redundant electromechanical actuation systems (EMAS) be designed to equal the flight safety reliability of dual tandem hydraulic actuators; and (4) can satisfactory solutions be found or developed for dissipating the heat generated in actuators and power controllers. The first question should be inconsequential since it is assumed that the all-electric aircraft will be equipped with a fly-by-wire (FBW) flight control system (FCS) which is already dependent upon an uninterrupted supply of electrical power. Design studies and hardware already developed show that the answer to question (2) is that EMAS outperform hydraulic actuators, particularly under load. The answer to question (3) is not as clear primarily because the issue has not been addressed in any depth. As posed the answer must be yes, but with the proviso that the weight might be greater than currently predicted. Studies have shown that we can cope with the heat dissipation issue addressed in question (4) in the case of motors and inverter/power controllers. The projections regarding usage of EMAS and the future of the all-electric airplane must be based on the type of vehicle (small subsonic transport, large transport, or military tighter) and the economics involved.
Material depth reconstruction method of multi-energy X-ray images using neural network (2012)

With the advent of technology, multi-energy X-ray imaging is promising technique that can reduce the patient's dose and provide functional imaging. Two-dimensional photon-counting detector to provide multi-energy imaging is under development. In this work, we present a material decomposition method using multi-energy images. To acquire multi-energy images, Monte Carlo simulation was performed. The X-ray spectrum was modeled and ripple effect was considered. Using the dissimilar characteristics in energy-dependent X-ray attenuation of each material, multiple energy X-ray images were decomposed into material depth images. Feedforward neural network was used to fit multi-energy images to material depth images. In order to use the neural network, step wedge phantom images were used for training neuron. Finally, neural network decomposed multi-energy X-ray images into material depth image. To demonstrate the concept of this method, we applied it to simulated images of a 3D head phantom. The results show that neural network method performed effectively material depth reconstruction.
PHASE I TRIAL OF A HUMANIZED, Fc RECEPTOR NONBINDING OKT3 ANTIBODY, huOKT3??1 (Ala-Ala) IN THE TREATMENT OF ACUTE RENAL ALLOGRAFT REJECTION (1999)

HuOKT3gamma1(Ala-Ala) possesses the ability to reverse vigorous rejection episodes in kidney and kidney-pancreas transplant recipients, and in comparison to murine OKT3, possesses minimal first dose reactions and does not seem to induce antibodies that bind the OKT3 idiotype. These results support the conduct of additional clinical trials with the huOKT3gamma1(Ala-Ala) antibody.
Recognition of Unique Carboxyl-Terminal Motifs by Distinct PDZ Domains (1997)

The oriented peptide library technique was used to investigate the peptide-binding specificities of nine PDZ domains. Each PDZ domain selected peptides with hydrophobic residues at the carboxyl terminus. Individual PDZ domains selected unique optimal motifs defined primarily by the carboxyl terminal three to seven residues of the peptides. One family of PDZ domains, including those of the Discs Large protein, selected peptides with the consensus motif Glu-(Ser/Thr)-Xxx-(Val/Ile) (where Xxx represents any amino acid) at the carboxyl terminus. In contrast, another family of PDZ domains, including those of LIN-2, p55, and Tiam-1, selected peptides with hydrophobic or aromatic side chains at the carboxyl terminal three residues. On the basis of crystal structures of the PSD-95-3 PDZ domain, the specificities observed with the peptide library can be rationalized.
Induction of Drought Stress Resistance by Multi-Functional PGPR Bacillus licheniformis K11 in Pepper (2013)

Drought stress is one of the major yield affecting factor for pepper plant. The effects of PGPRs were analyzed in relation with drought resistance. The PGPRs inoculated pepper plants tolerate the drought stress and survived as compared to non-inoculated pepper plants that died after 15 days of drought stress. Variations in protein and RNA accumulation patterns of inoculated and non-inoculated pepper plants subjected to drought conditions for 10 days were confirmed by two dimensional polyacrylamide gel electrophoresis (2D-PAGE) and differential display PCR (DD-PCR), respectively. A total of six differentially expressed stress proteins were identified in the treated pepper plants by 2D-PAGE. Among the stress proteins, specific genes of Cadhn, VA, sHSP and CaPR-10 showed more than a 1.5-fold expressed in amount in B. licheniformis K11-treated drought pepper compared to untreated drought pepper. The changes in proteins and gene expression patterns were attributed to the B. licheniformis K11. Accordingly, auxin and ACC deaminase producing PGPR B. licheniformis K11 could reduce drought stress in drought affected regions without the need for overusing agrochemicals and chemical fertilizer. These results will contribute to the development of a microbial agent for organic farming by PGPR.
Probiotics in inflammatory bowel disease: possible mechanisms of action. (2005)

Understanding the mechanisms responsible for the beneficial effect of probiotics in inflammatory bowel disease and experimental colitis may help understand the role of bacteria in disease pathogenesis. The findings that live probiotics may not be mandatory to be beneficial, and that therapeutic effects may be obtained by systemic, rather than oral administration could have a major impact on the practical use and manufacturing of probiotics.
Adaptable generic programming with required type specifications and package templates (2012)

The aim of this work is to provide better support for adaption and refinement of generic code. This type of flexibility is desirable in order to fully reap the potential of generic programming. Our proposal for an improved mechanism is an extension to the previously published Package Templates (PT) mechanism, which is designed for development of reusable modules that can be adapted to their specific purpose when used in a program. The PT mechanism relies on compile-time specialization, and supports separate type checking and type-safe composition of modules. The extension to PT presented here is called required types, and can be seen as an enhanced form of type parameters, allowing them the same flexibility as other elements of the PT mechanism. We implement a subset of the Boost Graph Library in order to exemplify, validate, and compare our approach to other options.
Effect of single and repeat doses of casopitant on the pharmacokinetics of CYP450 3A4 substrates midazolam and nifedipine (2010)

Casopitant is a dose- and duration-dependent weak to moderate inhibitor of CYP3A.