#  1\. PROJECT INFORMATION

##  Project name

Integrated and portable image cytometer for rapid response to _Legionella_ and
_Escherichia coli_ in industrial and environmental waters

Coordinator

Labaqua, S.A.

Grant Agreement Number

Nº 642356

Contact information

vicente.catalan@labaqua.com

##  Description

CYTO-WATER project is an innovation project co-founded by the European Union,
through the HORIZON 2020 initiative, call H2020-WATER-1-2014/2015: Bridging
the gap: from innovative water solutions to market replication.The project was
launched in June 2015 and it is expected to be completed in May 2018. The
project is coordinated by Labaqua in partnership with CETaqua, ICFO, Bertin
Technologies, microTEC and Memteq Ventures LTD. The objectiveof the project is
to deploy, for the first time in industrial and environmental waters, a new
imaging cytometer platform for the detection and quantification of
microorganisms. This will allow quantifying _Legionella_ and _E. coli_
population within 120 minutes from obtaining the sample, overcoming in this
way the main disadvantage of traditional methods used in laboratories, i.e.
long time-to results which can currently last up to 12 days in the case of
_Legionella_ and 1 day for _E. coli_ . This tool will be an easy-to-handle
portable form, which will increase its versatility and widen the possibilities
of onsite applications

#  2.DESCRIPTION OF DATA

The data generated may contain information that will be commercially sensitive
or subjected to patent or other IP applications. CYTO-WATERconsortium will use
reasonable efforts to ensure that as much data as possible are made accessible
to the research community; however this will be mitigated by the
aforementioned commercial considerations.

The data will be arriving as a continuous stream and stored in its raw form as
it is received from the individual partners. Given the heterogeneous sources
of CYTO-WATERdata, the consortiumwill keep all data in text files to maximize
their usability over platforms and time. Periodically, the existing data store
will be extracted, analyzed and archived, so that our overall data set will be
incremental in structure.

The expected data to be generated during this project include:

  * Output data from CYTO-WATER project research activities; 

  

  * Software source created by consortium members; and 

  

  * Reports on consortium work, including publications, presentations, demonstrations, courses,documentation related with the commercial promotion of the project and the CYTO-WATER system, etc. 

  

Additionally, the following type of data will also be stored:

  * Externally-generated research data used as inputs for research activities; 

  

It isrecognised that during the project development, other data sets may be
identified, assessed and stored by project participants. In such cases, the
DMP will be updated to reflect these additions.

In the following section all policies and activities planned for the
management and share of the data generated by CYTO-WATER project will be
addressed.

#  3.DATA MANAGEMENT

**Externally-generated research data(external to the consortium)** : Examples
of such data include laboratory data from researchers’ experimental work and
raw data from other facilities. Partners’facilities will not be the initial,
primary, or sole storage location for such data. Consequently, no special
provisions for such data areplanned, expecting that the rules for
preservation, dissemination, and sharing of such data will be principally set
and managed by the organizations that are responsible for, and have a stake
in, the initial generation of the data.

**Output data (Raw data)** : Output data includes experimental records
(statistics, results of experiments, measurements, observations from field
work, survey results, images, graphs), designs,congress presentations, etc.
The raw output of thisdata will be stored in a format designed to store and
organize large amounts of numerical data. The format willbe chosen to be
supported by many commercial and non-commercial software platforms, including
Java, MATLAB/Scilab, Octave, IDL, Python, R, Julia and Microsoft Office. The
participants of CYTO-WATER will follow the research data management policy of
the institutions generating the data. If the institution cannot keep the data
to the same standard as required by the coordinator’s data policy, the
coordinator will undertake to store the data under its policy terms.

**Software** : Software will be preserved in much the same way as the output
data.

**Reports:** The analyzed results obtained from the raw data files will be
stored in reports (in Word, PowerPoint or pdf format). Sharing and long-term
availability of the data isguaranteedby the Project coordinator (PC). Data
will initially be stored on local computers used during the measurements and
backed up in accordance with the procedures of the partner generating the
data. Additionally, upon decision of the partner, the relevant data/metadata
may beuploadedto a publicly available online repository, such as Zenodo (a
Website for Scientific publications that is especially suitable for EU project
data).

Labaqua, as PC,will encourage consortium partners to report frequently and
widely on their activities.A project website has been created in order to
allow public project reportsand outputscan bemade available to all interested
parties. In addition, as part of projectoutreach activities, a number of
demonstrations and similar activities will be conducted, and the content of
all of these results will be made available on the project website. Reports
will include deliverables of the project, validation reports, technical
specifications, manufacturing processes, modeling of these manufacturing
processes, and characterization of samples associated with these manufacturing
processes.

#  4.DATA SHARING

Data will be shared between partners withoutdelay. Outside of the consortium,
relevant data may also beshared, subject to commercial considerations, in
order to promote the benefits of the developed technology to the scientific
community, the end-users, and potential collaborators for future product
development.

A significant part of the measurement results (including images, graphs, etc.)
can beshared publicly. They demonstrate the performance of the technology
developed in the project, and can be considered a valuable input to other
researchers in the field, or a relevant content for dissemination purposes.

Technical designs and methods cannotbe shared publicly, because the
applicableintellectual property strategy is critical to enable commercial
advantage forsome of project partners.

Table 1 summarizes the main kind of data generated during the CYTO-WATER
project life, the level of privacy and the responsibility of the information.

Table 1: Main kind of data generated in CYTO-WATER project.  
  
<table>  
<tr>  
<th>

Level of privacy & access

</th>  
<th>

Data generated

</th>  
<th>

Short description

</th>  
<th>

Diffusion

</th>  
<th>

Responsibility

</th> </tr>  
<tr>  
<td>

Public

</td>  
<td>

Validations reports

</td>  
<td>

Testing of suitable Celltrap units for the project (Memteq)

</td>  
<td>

CYTO-WATER website

</td>  
<td>

Consortium members

</td> </tr>  
<tr>  
<td>

Results of end-users tests at the end of the project (Bertin)

</td> </tr>  
<tr>  
<td>

Results of experiments

</td>  
<td>

Comparisons of the different Celltrap units showing flow rates, sample
volumes, pressures, etc.

(Memteq)

</td>  
<td>

CYTO-WATER website

</td>  
<td>

Consortium members

</td> </tr>  
<tr>  
<td>

Private

</td>  
<td>

Technical specifications

</td>  
<td>

Celltrap membrane specifications for various sample types (Memteq)

</td>  
<td>

Consortium members

</td>  
<td>

Consortium members

</td> </tr>  
<tr>  
<td>

Description of technical and operation issues regarding the integration of the
entire platform

(IFCO)

</td> </tr>  
<tr>  
<td>

Observations from field work

</td>  
<td>

Environmental water samples vary in turbidity, color, solid contents and the
selection of Celltrap membrane types will become important (Memteq)

</td>  
<td>

Consortium members

</td>  
<td>

Consortium members

</td> </tr>  
<tr>  
<td>

Drawings of prototypes

</td>  
<td>

Drawings available for new Celltrap units (Memteq)

</td>  
<td>

Consortium members

</td>  
<td>

Consortium members

</td> </tr>  
<tr>  
<td>

Fluidic chip (microTEC)

</td>  
<td>

Consortium members

</td>  
<td>

microTEC

</td> </tr>  
<tr>  
<td>

Mechanical manufacturing drawings of the concentrator and electronic cards
manufacturing

</td>  
<td>

Consortium members

</td>  
<td>

Bertin

</td> </tr>  
<tr>  
<td>

</td>  
<td>

plans of the concentrator (Bertin)

</td>  
<td>

</td>  
<td>

</td>  
<td>

</td> </tr>  
<tr>  
<td>

Prototype samples

</td>  
<td>

Prototype samples (microTEC)

</td>  
<td>

Consortium members

</td>  
<td>

microTEC

</td>  
<td>

</td> </tr>  
<tr>  
<td>

Images

</td>  
<td>

Graphical data available (Memteq)

</td>  
<td>

Consortium members

</td>  
<td>

Consortium members

</td>  
<td>

</td> </tr>  
<tr>  
<td>

Validation reports

</td>  
<td>

Analysis of MicroTec's cartridges material to assess its performance with
ICFO's reader (ICFO)

</td>  
<td>

Consortium members

</td>  
<td>

Consortium members

</td>  
<td>

</td> </tr>  
<tr>  
<td>

Validation reports of the CYTOWATER system (Labaqua)

</td>  
<td>

Consortium members

</td>  
<td>

Labaqua

</td>  
<td>

</td> </tr>  
<tr>  
<td>

Survey results

</td>  
<td>

Market study (Bertin)

</td>  
<td>

Consortium members

</td>  
<td>

Bertin

</td>  
<td>

</td> </tr>  
<tr>  
<td>

Design reports

</td>  
<td>

Interface studies and integration studies (Bertin)

</td>  
<td>

Consortium members

</td>  
<td>

Bertin

</td>  
<td>

</td> </tr>  
<tr>  
<td>

Software

development reports

</td>  
<td>

Communications with other modules study (Bertin)

</td>  
<td>

Consortium members

</td>  
<td>

Bertin

</td>  
<td>

</td> </tr>  
<tr>  
<td>

Results of experiments

</td>  
<td>

Analysis of filter clogging tests, tests and analysis of biological and
physical recovery rates, and internal validation tests report of the
concentrator (Bertin)

</td>  
<td>

Consortium members

</td>  
<td>

Bertin

</td>  
<td>

</td> </tr>  
<tr>  
<td>

Evaluation of ICFO's reader performance on different turbidity level water
samples provided by

MemTec (ICFO)

</td>  
<td>

Consortium members

</td>  
<td>

Consortium members

</td>  
<td>

</td> </tr>  
<tr>  
<td>

Manufacturing processes

</td>  
<td>

Description of ICFO's reader assembling (ICFO)

</td>  
<td>

Consortium members

</td>  
<td>

Consortium members

</td>  
<td>

</td> </tr>  
<tr>  
<td>

Results of experiments

</td>  
<td>

Spread sheet with all the row data of the experiment performed

(Labaqua)

</td>  
<td>

Consortium members

</td>  
<td>

Labaqua

</td>  
<td>

</td> </tr>  
<tr>  
<td>

Experiments protocols

</td>  
<td>

Text document where all the procedures performed in the laboratory are written
(Labaqua).

</td>  
<td>

Consortium members

</td>  
<td>

Labaqua

</td>  
<td>

</td> </tr> </table>

##  Public data

All participants in the project will publish the results of their work to the
extent that the commercial interests of project results are preserved
according to the exploitation and business plans to be agreed among project
partners. Papers will primarily be published in peer-reviewed journals and/or
conference proceedings. The results may also appear in books written in
English.Primary data and other supporting materials created or gathered in the
course of the work will be shared with other researchers upon reasonable
request and within a reasonable time of the request. Main research information
and reports will be published on theproject website. Informative and
commercial publications focused on the marketing of the CYTO-WATER systems
will be written in Spanish, French, German and English at partner websites and
in other relevant sources (National and international conferences, publicity
in on-line journals such as _i-ambiente_ , _i-agua_ with high diffusion at
national and international level,brochures about CYTO-WATER project).The
emphasis of data management will be on faithful and reproducible record
keeping, with an emphasis on transparency and accountability in methods
utilized.

Results of the research will be made available in digital form in spreadsheet
tables, tab-delimited files, or image files. Images will be saved in standard
image formats such as JPEG, TIFF, or PNG. Main research products will be
available online in digital form. Manuscripts will appear in PDF, and contain
text, calculations, drawings, plots, and images. The targeted journals for the
results of this research project provide a downloadable PDF copy of the
manuscript on the web. In addition, the PC will link to these journal
publications from the project website’s “Publications” section.Validation
reports and technical specifications will be public through project website.

Details of the main research products will therefore appear in text, tables,
plots, and images in peer-reviewed journal articles and/or conference
proceedings. The results may also be included in book chapters. Patents will
be sought when relevant.

In summary, main public data will be the externally-generated research data
(external to the consortium),mostoutput data and reports (including scientific
reports, congress presentations, etc.), validation reports and technical
specifications will be showed at CYTO-WATER project webpage.

##  Private data

It is recognised by CYTO-WATER partners that certain data may be commercially
sensitive and thus the Consortium will withhold general access to those data
generated which may compromise such commercial sensitivity. Intellectual
Property issues restrict sharing of some of the data (designs, methods).
Filling and subsequent publication of the corresponding patent applications,
before making the data publicly available, would be a way of limiting such
restrictions.

The main private data of this research project are the development of
manufacturing processes, modeling of these manufacturing processes, and
characterization of samples associated with these manufacturing processes.

#  5\. ACCESS TO DATA AND DATA SHARING PRACTICES AND POLICIES

##  Period of data retention

Data will be made publicly availableon the project website, but the editing
rights will be granted only to the PC Data will not be embargoedbut will be
opened up for public use upon completion of the project step. This will allow
maximum transparency of the project and enable the maximum benefit to decision
makers, as one of the project goals is to improve public policy decision
making. Data will be made publicly available through the project site with
guides and or visuals/charts to increase the ability of the public to consume
the results of individual project steps.

Public access to research products will be regulated by the consortium in
order to protect privacy and confidentiality concerns, as well to respect any
proprietary or intellectual property rights. Legal offices will be consulted
on a case-by-case basis to address any concerns, if necessary. Terms of use
will include proper attribution to the PC and authors along with disclaimers
of liability in connection with any use or distribution of the research data.

##  Archiving and Preservation of Access

Research products will be made available immediately after publication.
Journal publications will be available online from respective journal websites
and linked to by the CYTO-WATER website. All data generated as a result of
this project will backed up daily to protect from loss of data from hardware
failures, fire, theft, etc.

Upon completion of the project, all data will be housed within the consortium
database and will be made available upon request in order to facilitate
maintenance and availability of project results. During a period of 5 years
after the project, results will be available upon request and the
responsibility will be of the project coordinator.

Initial data management and Sharing Plan