2. **Initial Data Management Plan**

  

  

**Project Data Contact:** Amelie Driemel ( _amelie.driemel@awi.de_ )
**Specification of the expected datasets created during SponGES:**

**2.1 Baseline environmental data**

**Data set description:**

During various campaigns with research vessel G.O. Sars (Norway) and probably
other vessels, various baseline datasets will be assembled:

  * CTD/Rosette (=>water chemistry profiles), 

  

  * Multibeam/Synthetic aperture sonars (=>bathymetry), 

  

  * Remotely Operated Vehicle (=>sea-bottom pictures) and 

  

  * Sediment cores (=> historic sponge spicule abundance, sediment chemistry, stratigraphic data) 

  

These datasets will serve as a baseline set for all Work Packages and define
the environmental context of the project. Due to the standard instrumentation
used, the datasets will be directly comparable to datasets from other
regions/areas (e.g. _https://doi.pangaea.de/10.1594/PANGAEA.753658_ .

**Standards and metadata:**

Standard oceanographic and geoscientific instrumentation will be used
(CTD/Rosettes, ROVs, gravity cores etc.) to obtain the data. The analysis of
ocean water and sediment will take place in the research institutes of the
consortium.

Raw data will be marked differently to quality controlled data and the latter
will be submitted to PANGAEA, data publisher for georeferenced data from earth
system research ( _www.pangaea.de_ ). The scientist will retain his work-file
until he is confident that the data quality is sufficient for archiving. After
that the data will be submitted to PANGAEA and an independent quality check
will be performed by the data curator of PANGAEA (units correct? parameter
names unique? metadata sufficient?). The data and metadata will then be
archived in PANGAEAs relational database. Metadata supplied to PANGAEA are:
Latitude/longitude, depth, date/time of sample, the PI, the authors of the
dataset, dataset title, methodology, link to article where data has been used,
specific comments if needed.

PANGAEA supports several metadata standards such as ISO19xxx series, DIF,
Dublin Core, Darwin Core etc. and supports existing metadata portals (e.g.
OAIster, ScientificCommons) to disseminate its data/metadata by using the OAI-
PMH and other standards.

**Data sharing:**

The use of citable and permanent DOIs for all datasets archived in PANGAEA
ensures the longterm availability of SponGES data.

The data will be access restricted during a moratorium period (password
protected datasets), but will already be archived in PANGAEA and will have the
project label "SponGES" for the fast identification of and search for project
data. For the baseline datasets a project access will be created in PANGAEA,
so that all project members can access and use (and cite) the same version for
their own work. At the latest after the moratorium the data will be freely
accessible, citable (DOI) and directly downloadable in tab format (at
_www.pangaea.de_ ).

All data will be shared by the Creative Commons Attribution 3.0 Licence
(CCBY).

**Archiving and preservation (including storage and backup):**

Long-term archiving (>10 years) and a backup of these datasets (and all costs
hereof) are guaranteed by the institutes operating PANGAEA (Alfred-Wegener
Institut, Bremerhaven and Center for Marine Environmental Sciences, Bremen),
see also information above. Each dataset will have a unique and persistent
DOI.

4

**Sponge genetic, metabolic and microbiological data**

**Data set description:**

The genome of selected sponge species will be analyzed (=> gene code data).
Furthermore, an analysis of the sponge-associated microbes/symbionts (16S
amplicon sequence data) will be conducted. Other expected data include:
Secondary metabolite gene clusters (nucleotide sequences) of for example PKS,
NRPS, saccharides, terpenes, siderophores, lantipeptides other
biotechnologically relevant secondary metabolite gene clusters (by ways of
metagenomics, metatranscriptomics) and enzyme-encoding genes (nucleotide
sequences) of for example halogenases and bioluminescent enzymes and other
biotechnologically relevant enzymes (by ways of metagenomics,
metatranscriptomics).

**Standards and metadata:**

State-of-the-art high-throughput sequencing facilities and the expertise
available in the consortium will be used to obtain the data.

Gene codes will be archived in a standard gene code repository such as
GenBank. Other associated data will be submitted to PANGAEA, data publisher
for georeferenced data from earth system research ( _www.pangaea.de_ ). The
link to the respective GenBank entry will be added to the datasets stored in
PANGAEA (example: _https://doi.pangaea.de/10.1594/PANGAEA.855513_ ). Metadata
supplied to PANGAEA are: Latitude/longitude, water depth, date/time of sample,
the PI, the authors of the dataset, dataset title, methodology, link to
article where data has been used, specific comments if needed.

PANGAEA supports several metadata standards such as ISO19xxx series, DIF,
Dublin Core, Darwin Core etc. and supports existing metadata portals (e.g.
OAIster, ScientificCommons) to disseminate its data/metadata by using the OAI-
PMH and other standards.

**Data sharing:**

The use of citable and permanent DOIs for all datasets archived in PANGAEA
ensures the longterm availability of SponGES data.

The data will be access restricted during a moratorium period (password
protected datasets), but will already be archived in PANGAEA (or GenBank). At
the latest after the moratorium the data will be freely accessible, citable
and directly downloadable in tab format.

All data will be shared by the Creative Commons Attribution 3.0 Licence (CC-
BY). (Exceptions could be datasets which are relevant to the biotechnological
potential of sponges, in which case the legal grounds have to be determined by
the institutes first).

**Archiving and preservation (including storage and backup):**

Long-term archiving (>10 years) and a backup of PANGAEA datasets (and all
costs hereof) are guaranteed by the institutes operating PANGAEA (Alfred-
Wegener Institut, Bremerhaven and Center for Marine Environmental Sciences,
Bremen), see also information above. Each dataset will have a unique and
persistent DOI.

GenBank also is an open access database aimed at the long-term availability of
nucleotide sequence data ( _http://www.ncbi.nlm.nih.gov/genbank/_ ). Each
dataset here has a unique GenBank

Identifier.

5

**Element flux data**

**Data set description:**

In in-situ and ex-situ experiments the element fluxes and budgets
(sources/sinks) of carbon, nitrogen and silicon through deep-sea sponge
grounds will be determined.

**Standards and metadata:**

Standard instrumentation will be used (e.g. benthic flux chambers, VacuSip
system for in-situ sampling) to obtain the data. The analysis of the water and
sediment samples will take place in the research institutes of the consortium.

Raw data will be marked differently to quality controlled data and the latter
will be submitted to PANGAEA, data publisher for georeferenced data from earth
system research. The scientist will retain his work-file until he is
confident, that the data quality is sufficient for archiving. After that the
data will be submitted to PANGAEA and an independent quality check will be
performed by the data curator of PANGAEA (units correct? parameter names
unique? metadata sufficient?). The data and metadata will then be archived in
PANGAEAs relational database ( _www.pangaea.de_ ). Metadata supplied to
PANGAEA are (for ex-situ experiments some do not apply): Latitude/longitude,
water depth, date/time of sample, the PI, the authors of the dataset, dataset
title, methodology, link to article where data has been used, specific
comments if needed.

PANGAEA supports several metadata standards such as ISO19xxx series, DIF,
Dublin Core, Darwin Core etc. and supports existing metadata portals (e.g.
OAIster, ScientificCommons) to disseminate its data/metadata by using the OAI-
PMH and other standards.

**Data sharing:**

The use of citable and permanent DOIs for all datasets archived in PANGAEA
ensures the longterm availability of SponGES data.

The data will be access restricted during a moratorium period (password
protected datasets), but will already be archived in PANGAEA and will have the
project label "SponGES" for the fast identification of and search for project
data. At the latest after the moratorium the data will be freely accessible,
citable (DOI) and directly downloadable in tab format (at www.pangaea.de). All
data will be shared by the Creative Commons Attribution 3.0 Licence (CC-BY).

**Archiving and preservation (including storage and backup):**

Long-term archiving (>10 years) and a backup of these datasets (and all costs
hereof) are guaranteed by the institutes operating PANGAEA (Alfred-Wegener
Institut, Bremerhaven and Center for Marine Environmental Sciences, Bremen),
see also information above. Each dataset will have a unique and persistent
DOI.

6

**Model data**

**Data set description:**

Various environmental and ecological models (generic, dynamic) will be
developed and applied on SponGES baseline data and on data obtained from the
literature (e.g. bycatch statistics, historic distribution of sponges,
physiology/biomass information). Resulting datasets will be e.g.:

  * Predictions of fishing impacts on sponge grounds 

  

  * Sponge recovery trajectories following significant disturbance scenarios 

  

  * Present and future species distribution models (maps of likely distribution) 

  

  * Dynamic food-web and biogeochemical model (to assess sponge-ecosystem functioning) The datatypes will either be maps, raster/shape files, or complete sets (zips files) of model codes and underlying data tables (e.g. as in: _https://doi.pangaea.de/10.1594/PANGAEA.842757_ ). 

  

**Standards and metadata:**

The documentation of the model used and the algorithms applied will be stored
together with the model output. Other metadata stored with the model data are
the PI, the authors of the dataset, dataset title, methodology, and the link
to the article where model data has been used (if applicable).

**Data sharing:**

The use of citable and permanent DOIs for all datasets archived in PANGAEA
ensures the longterm availability of SponGES data.

The model data will be access restricted during a moratorium period (password
protected datasets), but will already be archived in PANGAEA and will have the
project label "SponGES" for the fast identification of and search for project
data. For those model outputs needed for other work packages a project access
will be created in PANGAEA, so that all project members can access and use
(and cite) the same version for their work. At the latest after the moratorium
the data will be freely accessible, citable (DOI) and directly downloadable in
tab format (at _www.pangaea.de_ ). All data will be shared by the Creative
Commons Attribution 3.0 Licence (CC-BY).

**Archiving and preservation (including storage and backup):**

Long-term archiving (>10 years) and a backup of these datasets (and all costs
hereof) are guaranteed by the institutes operating PANGAEA (Alfred-Wegener
Institut, Bremerhaven and Center for Marine Environmental Sciences, Bremen),
see also information above.

Each dataset will have a unique and persistent DOI.

7