The fluorescence lifetime map of the most common disease causing variant of cystic fibrosis transmembrane conductance regulator (CFTR) tagged with enhanced cyan fluorescent protein (ECFP), F508-CFTR-ECFP (the middle image) flanked by the images of the wild type CFTR constructs tagged with ECFP. The F508-CFTR is a processing mutation that interferes with folding and trafficking of CFTR. This mutated version of CFTR prevents a close association between F508-CFTR and Epithelial Na+ Channel (ENaC) unless F508-CFTR is rescued by chemical correctors and low temperature (the second and the sixth images from the left). Also shown are the fluorescence lifetime images of negative and the positive FLIM constructs (the first image and the last images, respectively).