Drosophila ovary imaged with a Zeiss Lightsheet microscope. Ovarioles were mounted in agarose in a capillary tube, preserving the three-dimensional organization of the tissue. The muscle layer expresses Fasciclin 3 and Zasp, both visible as green GFP-tagged proteins. The actin cytoskeleton of the muscles and the egg chambers within the muscle layer are labeled with 568-Phalloidin (red), and nuclei are stained with DAPI (blue). Lightsheet microscopy is reviewed in this issue by Joseph Wolenski and Doerthe Julich. Cover image courtesy of Lynn Cooley’s lab in the Department of Genetics, Yale University.