5.C. Dnmt3a nuclear distribution pattern during in vitro and in vivo oocyte growth. Examples of digital images showing Dnmt3a distribution pattern visualized with a Cy3 secondary antibody (red), while DNA was counterstained with SYBR Green 14/I (green). Dnmt3a maintained a nuclear localization in all in vitro and in vivo classes of germ cells analyzed. (C) In vitro grown oocyte collected from an EA follicle which displays a surrounded nucleolus (SN) chromatin configuration: Dnmt3a is associated with DNA or gathered in clusters around it.