In-situ hybridisation allows an experimenter to localise the expression of a particular gene in the tissue/embryo of interest. The strength of this technique is that it can give resolution down to a cellular level without the need for specialised equipment and reagents. In this experiment we were looking for the MyoD mRNA, to see where and when te gene was expressed. We made this exeperiment to study muscle developement and growth. Our attention was focused on hypertrophic animals and their use in zootechnics, so we were studing muscles and the genes related to their growth. I'm a student in Agronomic and Environmental Biotechnology at the Università degli Studi di Perugia (University of Perugia), in Italy.