Differential interference contrast (DIC) image (the upper panel) of the single HEK293T cell transfected with wild type cystic fibrosis transmembrane conductance regulator (CFTR) tagged with enhanced yellow fluorescent protein (EYFP). The second row depicts the epifluorescence images of the same cell using different pseudocoloring modality of ImageJ (National Institutes of Health) image processing program. The bottom image shows the surface expression of the same cell as assessed by total internal reflection fluorescence (TIRF) microscopy (in collaboration with Drs. William Lee and Vladimir Parpura).