Original sentence: (2006) Eastern blotting was described again as a new technique for detecting glycoconjugates generated by blotting BSA onto PVDF membranes, followed by periodate treatment.The earliest example is Tanner and Anstee in 1976, where lectins were used to detect glycosylated proteins isolated from human erythrocytes.The membrane is then probed with antibodies for epitopes of interest.The principles used for Eastern blotting or glycans can be traced back to the use of lectins to detect protein glycosylation.Though a commonly employed set of techniques, the term Eastern blotting is used only rarely in primary literature, and has appeared more in recent publications, sometimes referring to significantly different versions of the experiment
Edited sentence: (2001) Eastern blotting was described as a new technique for detecting glycoconjugates generated by blotting BSA onto PVDF membranes, followed by periodate treatment., and makes the claim that Eastern blots "don't exist," and have been first introduced by the 2007 paper discussed.A recent highlight article has interviewed Ed Southern, originator of the Southern blot, regarding a re-christening from Tanaka et al.The earliest example for this mode of detection is Tanner and Anstee in 1976, where lectins were used to detect glycosylated proteins isolated from human erythrocytes.The membrane is then probed with antibodies for epitopes of interest.. This method has also been discussed in later work by the same group.The principles used for Eastern blotting to detect glycans can be traced back to the use of lectins to detect protein glycosylation.This statement seems to be in direct contradiction with the reference list of the very paper being discussed which cites a 2001 publication with the phrase "a new eastern blotting technique" in its title.Though a commonly employed set of techniques, the term Eastern blotting is used only rarely in primary literature, which may help explain the significant differences between variations of experiment