分类：社会民生-求职就业
问题：20分献上。请帅哥美女帮忙翻译篇摘要，大恩不言谢！！！桉树抗寒基 
桉树抗寒基因高效转化体系的研究摘 要桉树抗寒基因高效转化体系的研究是在桉树高效转化体系的基础上，以暗培养3-5天的桉树茎尖为受体，用含有沙冬青抗寒基因AmGS的农杆菌浸染，进行3因素（DNA浓度，乙酰丁香酮和浸染时间）3平的正交试验，通过在含有卡那霉素的培养基上进行抗性筛选，观察其成活率，及时抢救污染茎尖，经过10天的一筛后转入二筛培养基，并定期进行观察，随时记录相关参数的调查统计，采用极差和方差分析，以获取高效转化体系的最佳实验方案。其最佳组合为茎尖+DNA原液+乙酰丁香酮25mg/L+浸染时间10min+共培养5天。
回答：Abstract :Ealyptus cold tolerance gene on the efficient conversion of system,Eucalyptus efficient transformation system as a basis,In the darkroom to develop 3 -5 days of the shoot tips of eucalyptus receptor,Holly containing sand cold tolerance gene AmGS of Agrobacterium infection, three factors (DNA concentration, acetosyringone and disseminated time), three-level orthogonal test, Adopted in the presence of the kanamycin resistance medium for screening,Observe their survival,Timely rescue pollution eucalyptus shoot tip,After 10 days after the first screening to the second screening medium,And regular observation and recording parameters survey,Used very poor and the variance analysis, in order to obtain efficient transformation system the best experimental program.The best combination ofShoot tips + DNA Solution + Acetosyringone25mg/L+ disseminated time 10min+Cultivation 5 days .不要意思!专业词汇和术语太多了,不妥之处请多多原谅,上述翻译算是供你参考吧!