www.helmholtz-hzi.de P04: Chemical and biological diversity of secondary metabolites from basidiomycetes. Blondelle Matio Kemkuignou1, Khadija Hassan1, Kathrin Wittstein1, Clara Chepkirui1, Ashaimaa Y. Moussa2, Cony Decock3, Josphat C. Matasyoh4, Marco Kirchenwitz5, Theresia E. B. Stradal5, Monique Rascher-Albaghdadi6, Reinhard W. Köster6, Marc Stadler1,* 1Department of Microbial Drugs, HZI, Inhoffenstrasse 7, 38124 Braunschweig, Germany 2Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, 11566 Cairo, Egypt 3Mycothéque de l’Université Catholique de Louvain (BCCM/MUCL), B-1348 Louvain-la-Neuve, Belgium 4Department of Chemistry, Egerton University, P.O. Box 536, Njoro 20115, Kenya 5Department of Cell Biology, HZI, Inhoffenstrasse 7, 38124 Braunschweig, Germany 6Department of Cellular and Molecular Neurobiology,TU Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany New Amylosporanes A-G (1-7) isolated from Amylosporus sp. together with 8 previously reported compounds C H3 CH3 C H3 CH3 CH3 OH OH O H O H H 1 C H3 CH3 C H3 CH3 CH3 OH OH O H O 1 OH OH Cl O H N NH O O H OH OH H O OH OH H OH O H H OH H OH O OH 12 13 14 10 9 15 11 8 3 5 1 8 1'' 2'' 1' 2' 5' 4' OH OH 7 O H O 4'' 5'' 3 5 1 1'' 2'' O 8 1' 2' 5' 4' 7 O OH OH 5'' 4'' O H 3 4 6 1 1'' 2'' 5'' 4'' OH 8 7 O OH O 1' 2' 5´ 4´ O H 3 1 5 1´ 2´ 5´ 4´ O O 1´´ 2´´ OH 5´´ 4´´ 7 1 2 3 7 5 6 3 5 1 1'' 2'' 5'' 1' OH OH 8 7 4'' 2' 5' 7' 4' 10' 9' OH O (E) (E) (E) (E) 3 5 1 1'' 2'' 5'' 4'' 1' 2' 4' 5' H OH OH 8 1´ 7 3 6 5 O H O O O 1 2´ 4´ 5´ 3 2 4 5 O 6 OH 1 O 1´ 3´ 4´ 5´ 3 5 1 OH OH 7 O OH 8 1´ 2´ 5´ 4´ 7´ 10´ 9´ H (E) (Z) 3 5 1 1´´´ 2´´´ 5´´´ 7´´´ 10´´´ 9´´´ 1´´ 5´´ 4´´ O 1´ 5´ 4´ 7 O O H 8 OH 4´´´ 4 (E) Orsellinic acid-derived carbons 1H-1H COSY 1H-1H NOESY 1H-13C HMBC Structure Elucidation The structures of the compounds were determined using spectroscopic methods (HR-ESIMS, UV, IR, CD, 1D and 2D NMR) Introduction Natural products have played a key role in drug discovery, especially for cancer and infectious diseases. However, the rampant rise of antimicrobial resistance to known drugs is the biggest threat to public health worldwide, which necessitates the search for hitherto untapped sources of novel, effective drugs. Basidiomycota has been recognized as a prolific source of secondary metabolites, with prominent and selective biological activities even though it remains widely underexplored. In particular, not only African relatives of the traditional edible and medicinal Asian mushrooms such as Sanghuangporus and Laetiporus but also rare and endemic tropical species of genera that have never been studied before for bioactive constituents, such as Heimiomyces, have recently yielded interesting secondary metabolites. This study describes the evaluation of Amylosporus cf. campbelii and Amylosporus cf. graminicola that were isolated from specimens collected in Zimbabwe and Cuba, respectively, and from which no secondary metabolites have hitherto been reported. Extracts of the strains of the Amylosporus spp. showed moderate antibacterial effects in our screening for novel anti-infective metabolites along with a unique HPLC/ DAD-MS metabolite profile, and we selected these fungi for an evaluation of the active principles. [1] Additionally, the neurotrophic properties of triterpenes isolated from fruiting bodies of Laetiporus sulphureus [2] and a mycelial culture of Antrodia sp. MUCL 56049 was investigated. Several triterpenes potently stimulated the expression of neurotrophic factors, namely, NGF (sulphurenic acid, 15α-dehydroxytrametenolic acid, fomefficinic acid D, and 16α-hydroxyeburicoic acid) and bdnf (sulphurenic acid and 15α-dehydroxytrametenolic acid). The triterpenes also potentiated NGF-induced neurite outgrowth in PC-12 cells. This is, to the best of our knowledge, the first report on the compound class of lanostanes in direct relation to bdnf and ngf enhancement [3]. L. sulphureus Photo: Marc Stadler Laetiporus sulphureus Antrodia sp. MUCL 56049 Am