BUTANEXT: NEXT GENERATION BIOBUTANOL BUTANEXT CHALLENGES  Develop a two-step (mechanical-micronising & chemical-enzymatic pretreatment technology) that is versatile (works with different types of lignocellulosic feedstocks and wastes including recalcitrant materials) and low cost (reduces energy demands between 15-25%) both in terms of CAPEX and associated downstream OPEX for hydrolytic processing  Reduce enzyme dosage and costs (up to 25%) using a boosting cocktail, adapted to the Clostridium butanol fermentation process.  Increase fermentation efficiency by improving the butanol tolerance using non-GMO techniques to produce homo- fermentative next generation microorganisms, high productivity fermentation with in-situ product recovery (ISPR). The project targets a 2-fold increase in volumetric productivity versus a conventional ABE batch process.  Validate the technical performance of the whole integrated process from feedstock handling to product recovery in a centralised pilot facility (100L fermentation scale).  Perform a techno-economic and sustainability assessment of the integrated process in order to evaluate and guide further process developments and upscaling. ACKNOWLEDGEMENTS  MetGen Oy for the supply of enzymes for enzymatic hydrolysis assays.  The ButaNexT project has received funding from the European Union Horizon 2020 Research and innovation Programme under grant agreement n° 640462 Inés DEL CAMPO/ Irantzu ALEGRIA/ David SANCHEZ National Renewable Energy Centre (CENER) – Biomass Department. C/ Ciudad de la Innovación 7. 31621 Sarriguren, Navarra (Spain). Tel: +34-948-25-28-00. / idelcampo@cener.com/ialegria@cener.com/ dsanchez@cener.com Tim Davies/ Jenna Hewitt.Greenbiologics Ltd. ButaNexT@greenbiologics.com. Alvaro Pallares/Clemente Garcia. Técnicas Reunidas.apallares@tecnicasreunidas.es /cgredondo@tecnicasreunidas.es. Heleen De Wever/ Wouter Van Hecke. Vlaamse Instelling Voor Technologisch Onderzoek N.V.(VITO). heleen.dewever@vito.be / Wouter.vanhecke@vito.be. Magìn Lapuerta/David Fernández. University of Castilla la Mancha. Magin.Lapuerta@uclm.es / davidfdez232@gmail.com. Lucy Natrass. E4tech Limited. Lucy.natrass@e4tech.com. Bob Crawford. C-TECH Innovation Limited. bob.crawford@ctechinnovation.com. Juan Sanciñena. Zabala Innovation Consulting. JSancinena@zabala.es / Guillaume Corradino. Greenovate Europe. Guillaume.Corradino@greenovate.eu THE PROJECT TEAM BUTANEXT OBJECTIVES ButaNexT aims to develop and validate a cheaper, more energy saving, social and environmentally friendly integrated process able to efficiently convert sustainable feedstocks (lignocellulosic biomass and wastes) into biobutanol and define optimal biobutanol blends with fossil fuels and conventional biofuels (ethanol and biodiesel). Overall, the project aims to: • Validate for a relevant commercial concept significant cost reductions in biobutanol production vs 1st generation biobutanol production using the ABE process with starch. ButaNexT aims to reduce costs by up to 50% (targeting $800/T). The aim is to achieve price parity with the “best in class” bioethanol and biodiesel production in the EU. • Demonstrate enhanced energy balances and reduced carbon emissions vs 1st generation biofuel production. A reduction of 85% GHG emissions compared with fossil fuels (gasoline) is targeted. • Demonstrate butanol superior properties as fuel extender by optimising blends with gasoline and diesel and conventional biofuels (ethanol & biodiesel) to improve their performance characteristics and reduce emissions. The most promising diesel blends will be tested for performance and emissions in a diesel engine. • Demonstrate process sustainability via the utilisation of low cost and sustainable feedstocks (agricultural residues, energy crops and municipal wastes), the improvement of process conversion efficiencies (sugar and solvent yields and productivity) and the reduction of the environmental footprint (reduced energy requirements, water usag