Data Output Type 1_Experimental Level. Each evaluation question will have ONE response per experiment, making a fixed total of questions equivilant to the number experiments Category Study Quality Evaluation Question Evaluation Result Test compound and controls Is the chemical name, ID, or CAS number of the test compound (s) provided? Fulfilled Test compound and controls Is the purity of the test compound stated or able to be determind by the information provided? Fulfilled Test compound and controls Is the solubility of test chemicals in the assay system described? Fulfilled Test compound and controls Negative, untreated or vehicle control was included? Fulfilled Test compound and controls Concentration of vehicle was provided? Fulfilled Test compound and controls It was stated that a reference compound or positive control was included? Fulfilled Test compound and controls Information on assay reagents (Product name, supplier and lot) was provided? Fulfilled Total 7 Test System The tests and/or methods used were sufficiently described to allow for evaluation of reliability of results? Fulfilled Test System Conditions (Inclubation temperature, humidity, CO2 concentration, etc.) was described? Fulfilled Test System The androgen receptor source and characteristics(recombinant, native, etc. was described? Not fulfilled Test System The cell system was described? Fulfilled Test System The number of cell passages for the cell line used and the number of cells (density) used during the treatment was described? Not fulfilled Test System The composition of media (use of serum, antibiotics, etc.) was described? Fulfilled Test System The species in which the cell line/test system originiated from is stated or able to be determind by description provided? Fulfilled Test System The test system (cell line/tissue/etc., whole-cell vs. cell-free) was described? Partially fulfilled Test System Were the methods either described in detail or in another publication to which a clear reference is made? Fulfilled Total 9 Funding and competing interests The funding sources for the study were stated? Fulfilled Funding and competing interests Any competing interests were disclosed or it was explicitly stated that the authors did not have any competing interests. Fulfilled Total 2 Administration of test compound Duration of treatment period was provided? Fulfilled Data Output Type 2_Outcome Level. Each evaluation question will have ONE response per experiment-chemical, making a variable total of questions per relevant experiment. Category Evaluation criteria Evaluation Result Administration of test compound It was stated that the effect of the test compound on cytotoxicity was measured? Fulfilled Administration of test compound The administered dose levels or concentration of treatment chemical was stated? Fulfilled Administration of test compound Were all test chemicals tested over a multiple concentrations? Fulfilled Administration of test compound The number of replicates per dose/concentration used or number of times the experiment was repeated was stated? Fulfilled Total 5 Data collection and analysis Acceptability and data interpretation criteria were provided? Fulfilled Data collection and analysis Do the author or reviewer report any concerns with the data reported? Fulfilled Data collection and analysis Measures of variability between replicates of a treatment group is reported (e.g. SD, SE, %CV, 95% CI, etc.)? Not applicable Data collection and analysis The time points for data collection were stated? Fulfilled Total 4 Background Literature Searching & Screening Development of a Curated, Cross-Species Androgen Receptor Database aU.S. EPA, ORD/CCTE/SCDCD, Duluth, Minnesota; bU.S. EPA, OCSPP/OPP, Washington, DC; cU.S. EPA, ORD/CCTE/GLTED, Duluth, Minnesota Sara M.F. Vliet a, Kristan J. Markey b, Scott G. Lynn b, Carlie A. LaLone c • The US Environmental Protection Agency (EPA) is tasked with assessing chemicals for their potential to adversely im