An Insight Into the Interaction of Oxo-Vanadium (V) Complex with Nucleic acid Basel Life Science Week & MipTech Exhibition 2015 Mamta Tripathi and Rama Pande* School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur, 492010, Chhattisgarh, INDIA E mail ID - mamtat320@gmail.com INTRODUCTION  Nucleic acids are targeted as they are the central machinery for protein synthesis.  DNA being the starting material in central dogma leads to the formation of RNA which in turns produces proteins in body.  Molecule that can bind to DNA/RNA plays a key role in drug designing mechanism.  Mode of interaction predict the potency and extent of binding molecule. FUTURE ASPECTS REFRENCES N-ARYLHYDROXAMIC ACID OBJECTIVE FLUORESCENCE METHOD MODES OF INTERACTION ACKNOWLEDGEMENT CONCLUSION Co-Researcher Yamini Thakur Bharati Verma Rubi Khilari Manish Pardhi Shakuntala Raj I am thankful to Prof.(Mrs.) Rama Pande, Supervisor for her ever available guidance and support . I am very grateful to DST and UGC for providing financial assistance.  R. Khilari, Y. Thakur, M. Pardhi, R. Pande, Nucleosides, Nucleo- tides and Nucleic Acids, 34 (2015) 332-347.  P. Singh, D. Khare, R.Pande, Chem. Pap. 68 (2014) 1298-1304.  B. Verma, M. Pardhi, R Pande, Int J Pharm Pharm Sci. 61 (2014) 170-174.  J.I.B. Janairo, G.C. Janairo, Analele Universităţii din Bucureşti – Chimie (serie nouă) 20 (2011) 25 – 30.  P.J. Cox, E.J. Gibbs, C.A. Bolos, J. Bioorg. Med. Chem. 17 (2009) 6054–6062. Determination of following parameters:  Nucleic acid interactional studies of different metal complex of Hydroxamic acid.  Enzymatic Inhibitory Activity .  Antioxidant property.  UV absorption spectra shows hyperchromic Shift with slighter blue shift with Nucleic acid .  Decrease in intensity of emission spectra (fluorescence quench- ing & enhancement) indicates the binding of molecule with nu- cleic acid.  Ethidium bromide displacement shows decrease in intensity of emission spectra indicating intercalation mode of binding and in- crease in intensity of spectra interpreted as groove binding.  The relative viscosities of complex shownhave increased value as compared to ct-DNA/t-RNA alone which further confirm the binding.  Hydroxamic acids (RC(=O)N(OH)R′) used in the present investigation are drug like molecules and a very important class of bio-ligands .  Possess both hydrogen bond donor and hydrogen bond acceptor sites and usually form stable five membered [O,O] chelating agents. with metal ions. To Investigate the interactional parameters of Oxo-chloro-bis-N- phenylbenzohydroxamato-vanadium(V) ,PBHA-V(V)- with Nucleic acid by Absorption Spectroscopy, Fluorescence Spectroscopy, Viscos- ity measurement and Molecular Docking Techniques. EXPERIMENTAL SECTION Molecular formulae : C26H20ClN2O5V Molecular weight : 526.84 Structure : Oxo-chloro-bis-N-phenylbenzohydroxamato-vanadium(V) ,PBHA-V(V)- N C O O N C O O V O Cl Solution Preparation  The stock solutions of hydroxamic acid, (0.01M) were prepared in DMSO and used further of various concentrations as obtained by mass dilution technique. SOLUTION OF DNA/RNA  The stock solution of RNA was prepared in citrate-phosphate buffer and used further of various concentration whereas DNA Solution was prepared in Tris-HCl buffer. 220 240 260 280 300 320 340 360 -0.1 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 Absorbance (a.u.) Wavelength (nm) 200 220 240 260 280 300 320 -0.5 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Absorbance (a.u) Wavelength (nm) Representative UV-Vis spectra recorded for (a) PBHA-V(V) -ct-DNA ,(b) PBHA- V (V)-t-RNA with increasing concentration of Ct-DNA and t-RNA at 298 K .  Fluorescence quenching is decrease in fluorescence intensity of lumi- nescent species with interaction to other species.  Binding constant Ksv were calculated by Stern—Volmer Equation. F0/F= 1+ KSV (Q) Where, F0 = the fluorescence intensities of the PBHA-V(V) with ct-DNA/t -RNA the absence of quencher. F = the fluorescence intensities of th