Question: I'm working on automating GUI testing using OpenCV and PyAutoGui. I tried both pyautogui.locateOnScreen() and cv2.matchTemplate() to detect UI elements by matching a reference image inside a screen region. The reference image is visibly present inside the screenshot (confirmed manually), but both approaches either fail or return very low confidence (~0.21), and no click is triggered.   The image path is passed via sys.argv.  if __name__ == "__main__": if len(sys.argv) < 3: print("[ERROR] Usage: python OCR.py <word> <confidence>") else: word = sys.argv[1] reference_image = sys.argv[2] result = find_and_click_text(word, reference_image) print(result)   Here's what works:  If the path is hardcoded inside the .py script → locateOnScreen() works perfectly. If I pass the image path via sys.argv, and print it → it looks identical. Image.open(reference_image) works — file exists and loads fine.   Here's what fails:  pyautogui.locateOnScreen(reference_image) → fails silently or gives low confidence (~0.2) Even if I pass the loaded image object (Image.open(path)) to locateOnScreen() → it fails OpenCV's cv2.matchTemplate() also gives low confidence (~0.21) even though the reference image clearly appears in the screenshot.   .py File Output: [DEBUG] Reference image path received: C:\PFE\Test_Automation\CubeMX\PA6_reference.png [DEBUG] sys.argv: ['OCR.py', 'GPIOOutput', 'C:\\PFE\\Test_Automation\\CubeMX\\PA6_reference.png'] [DEBUG] File exists at: C:/PFE/Test_Automation/CubeMX/PA6_reference.png [ERROR] Traceback (most recent call last): File "C:\Python312\Lib\site-packages\pyautogui\__init__.py", line 172, in wrapper return wrappedFunction(*args, **kwargs) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "C:\Python312\Lib\site-packages\pyautogui\__init__.py", line 210, in locateOnScreen return pyscreeze.locateOnScreen(*args, **kwargs) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "C:\Python312\Lib\site-packages\pyscreeze\__init__.py", line 405, in locateOnScreen retVal = locate(image, screenshotIm, **kwargs) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "C:\Python312\Lib\site-packages\pyscreeze\__init__.py", line 383, in locate points = tuple(locateAll(needleImage, haystackImage, **kwargs)) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "C:\Python312\Lib\site-packages\pyscreeze\__init__.py", line 257, in _locateAll_opencv raise ImageNotFoundException('Could not locate the image (highest confidence = %.3f)' % result.max()) pyscreeze.ImageNotFoundException: Could not locate the image (highest confidence = 0.274)   What I’ve Confirmed:  The problem isn't solved even when I limit the search area using pyautogui.screenshot(region=(...)) ImageChops.difference() confirms the reference and cropped screen area are pixel-perfect The reference image isn't accessed after running the script.   Screenshots: The first image is my screen and the second is the reference image.   Thanks in advance — I’m happy to share any additional information (code + screenshots) if needed.

Answer: Here is your template overlaid side by side with the instance I think you want to locate.  See how the image content is not the same size? That is the problem. That can't match. It has to be the same size/scale. Pick the template correctly. Then it will work.