## 4 Discussion  
### Bacterial Community  
The two strategies of analysis (culture-dependent and culture-independent) evidenced a link with the members of the genera _Microococcus_ and _Paenibacillus_. These bacteria were detected by both approaches, but in different samples. They are frequently isolated from historical textile [1; 20] and also in the past studies, as well as here, they showed significant hydrolytic properties. Members of the genus _Paenibacillus_ produce a broad range of enzymes used in biotechnology processes [21]. In addition to _Microococcus_, other isolated Actinobacteria with interesting proteolytic and fibroinolytic properties belonged to the genus _Kocuria_. Representatives of this genus are commune in subterranean environment [22] and were also isolated from archaeological textile [23]. Therefore, bacteria of the genera _Paenibacillus_, _Microococcus_, and _Kocuria_ can be considered as dangerous textile degrading microorganisms. By high-throughput sequencing the genera _Microoccus_ and _Paenibacillus_ were dominant in the sample c (tissue covering the stiff reinforcement), where _M. luteus_ comprised 8.47% of all reads classified. _Microoccus luteus_ is also one of the species inhabiting the human skin [24]. Members of the genus _Microoccus_ were also previously identified as part of microbiota on mummy surface [2]. Regarding the MinION analysis, three species of the genus _Cutibacterium_ (_C. acnes_, _C. granulosum_, and _C. avidum_), formerly known as _Propionibacterium_[25], were detected in all four samples (a, b, c, and d) with the remarkable prevalence of _Cutibacterium acnes_, which is involved in the maintenance of a healthy skin, but it can also act as an opportunistic pathogen in acne vulgaris [26] or can cause implant-associated infections [27]. Genus _Acinetobacter_ dominated mostly in sample b (sampled from silk fabric of the bodice), whose probably touched the torso of cadaver. [PERSON] et al. [28] observed the highest abundance of _Acinetobacter_ in samples of male (5-20% of relative abundance) and female (5-15% of relative abundance) cadavers studied during the time, mostly in the late stages of their decomposition. The genus _Brevibacterium_ dominated in the samples b and c, namely _B. casei_ (5.63%) and B. pityocampae (4.15%), respectively. _Brevibacterium_ was the most frequent genera identified in human archaeological remains by [PERSON] et al. [29], also typically found in a wide range of soils and waters. The genus _Pseudonocardiia_ was in our case the most abundant (silk bobbin lace cap) in sample d (7.06%). This genus was previously detected in the caves [30], catacombs [31], tomb walls, as part of the ancient paintings deteriorating microflora [32], or part of the so-called moonmilk [33] and also on the body of a mummy [2]. The reads identified as genus _Pedomicrobium_ (Alphabacteria class) predominated in samples b and d (2.67% and 5.42%, respectively). _Pedomicrobium_ is known as iron- and manganese-oxidizing and accumulating bacterium often detected in biofilms formed on different surfaces [34; 35]. Probably, in these samples _Pedomicrobium_ could be responsible for various corrosion phenomena [36] of metal materials present in the structure of the bodice, but also as decoration jewels on the head of girl. The isolates _Roseomonas mucosa_ and _Sphingomonas_ sp. also belonged to the class of Alphabacteria. Here they contribute to the degradation of silk fibroin and other kind of proteinaceous substrates. These bacteria have the characteristic to possess hydrolytic enzymes active also at low temperature [37; 38]. The iron-oxidizing bacteria of the genus _Sediminibacterium_ were present with approximately 2% in all samples, except of sample c. We think that their role is connect mainly with the corrosion [39] of metal materials present in these samples. In the samples a and c, _Brachy bacterium_ was dominated by reaching 3.73% and 4.19%, respectively.