## Results
### Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR)
\begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline \multirow{2}{*}{**Protein**} & \multicolumn{2}{c}{**ORF 1 (bp)**} & \multirow{2}{*}{**Aa 2**} & \multirow{2}{*}{**M.W. 3**} & \multirow{2}{*}{**pl 3**} & \multirow{2}{*}{**Subcellular Localization Predictions**} & \multirow{2}{*}{**Secondary Structure Elements**} \\  & & & & & & & **TargetP** & **ProtComp 9.0** & & **TMH 5** & \(\alpha\)**-H 6** & \(\beta\)**-S 6** \\ \hline \multirow{2}{*}{AACT} & 1239 & \multirow{2}{*}{412} & \multirow{2}{*}{43.2} & \multirow{2}{*}{8.64} & \multirow{2}{*}{0.458 mT 4, 0.036 SP 4, 41.4 Other} & \multirow{2}{*}{ Mitochondrial} & \multirow{2}{*}{No TMH 1} & \multirow{2}{*}{11} & \multirow{2}{*}{16} \\  & MK387135 & & & & & & & & \\ \hline \multirow{2}{*}{HMGS} & 1374 & \multirow{2}{*}{457} & \multirow{2}{*}{50.7} & \multirow{2}{*}{6.04} & 0.0172 mT 0.643 SP, 0.648 Other & \multirow{2}{*}{Cytoplasmic} & \multirow{2}{*}{No TMH 1} & \multirow{2}{*}{15} & \multirow{2}{*}{17} \\  & MK387139 & & & & & & & & \\ \hline \multirow{2}{*}{HMGR} & 2550 & \multirow{2}{*}{849} & \multirow{2}{*}{93.4} & \multirow{2}{*}{8.07} & 0.124 mT 0.471 SP, 0.398 Other & \multirow{2}{*}{\begin{tabular}{} \end{tabular} } & \multirow{2}{*}{\
\begin{tabular}{} \end{tabular} } & \multirow{2}{*}{5 TMH 1} & \multirow{2}{*}{16} & \multirow{2}{*}{11} \\  & MK387135 & & & & & & & \\ \hline \multirow{2}{*}{MK} & 1308 & \multirow{2}{*}{435} & \multirow{2}{*}{48.3} & \multirow{2}{*}{6.17} & 0.497 mT 0.046 SP, 0.579 Other & \multirow{2}{*}{Cytoplasmic} & \multirow{2}{*}{No TMH 1} & \multirow{2}{*}{11} & \multirow{2}{*}{13} \\  & MK387142 & & & & & & & \\ \hline \multirow{2}{*}{PMK} & 558 & \multirow{2}{*}{21.3} & \multirow{2}{*}{6.89} & 0.092 mT 0.077 SP, 0.881 Other & \multirow{2}{*}{Perosional} & \multirow{2}{*}{No TMH 1} & \multirow{2}{*}{8} & \multirow{2}{*}{5} \\  & MK387143 & & & & & & & \\ \hline \multirow{2}{*}{MDPC} & 1161 & \multirow{2}{*}{386} & \multirow{2}{*}{42.9} & \multirow{2}{*}{7.01} & 0.058 mT 0.275 SP, 0.748 Other & \multirow{2}{*}{Extracellular} & \multirow{2}{*}{No TMH 1} & \multirow{2}{*}{11} & \multirow{2}{*}{17} \\  & MK387141 & & & & & & & \\ \hline \multirow{2}{*}{IPPI} & 759 & \multirow{2}{*}{28.9} & \multirow{2}{*}{6.31} & 0.4348 mT 0.133 SP, 0.404 Other & \multirow{2}{*}{Cytoplasmic} & \multirow{2}{*}{No TMH 8} & \multirow{2}{*}{8} & \multirow{2}{*}{9} \\  & MK387141 & & & & & & & \\ \hline \multirow{2}{*}{GPPS/FPPS} & 1290 & \multirow{2}{*}{429} & \multirow{2}{*}{49.5} & \multirow{2}{*}{8.7} & 0.778 mT 0.063 SP, 0.223 Other & \multirow{2}{*}{
\begin{tabular}{} \end{tabular} } & \multirow{2}{*}{No TMH 1} & \multirow{2}{*}{16} & \multirow{2}{*}{2} \\  & MK387136 & & & & & & \\ \hline \multirow{2}{*}{GGPPS} & 894 & \multirow{2}{*}{297} & \multirow{2}{*}{34.3} & 0.108 mT 0.091 SP, 0.855 Other & \multirow{2}{*}{Cytoplasmic} & \multirow{2}{*}{No TMH 15} & \multirow{2}{*}{2} \\  & MK387136 & & & & & & & \\ \hline \multirow{2}{*}{\({}^{1}\)Open Reading Frame. 2} & \multirow{2}{*}{Number of amino acids defined by the ExPASy Translate tool. 3 Molecular Weight and Isoelectric point predicted with ProtParam. 4 mTP: mitochondrial, SP: secretory pathway. 5 Predicted number of transmembrane helices (TMH) by the TMHMM Server v. 2.0. 6 Number of \(\alpha\)-helices (\(\alpha\)-H) and \(\beta\)-sheets (\(\beta\)-S) assigned by the ESPript 3.0 program.  
\end{table} 
Table 2: Physicochemical properties, cellular localization, and secondary structure elements predicted for _D. rhizophagus_ MVA pathway’s putative proteins.