## 3 Results and Discussion
### Gas Chromatography-Mass Spectrometry (GC-MS) Analysis  
The presence of chemical components in the methanol extract obtained by maceration of _B. hispanica_ was identified by GC-MS after methylation. The results obtained showed that forty-five chemical compounds were identified in the extract (Figure 3; Table 2), among them 2-heptnal, (Z); 2,4-decadiena; 2,4-decadien; heptadecane; 2,6,10,14-tetramethyl; hexadecane; and 11,14-eicosadienoic acid methyl ester were the chief chemical compounds in the plant extract. The findings of chemical analysis obtained with GC-MS displayed in Table 2 agreed with the results of the total polyphenic and flavonoid contents presented in Table 1, since both affirm the presence of common chemical classes. Figure 1: Gallic acid calibration curve used for total phenol quantification. Figure 2: Quercetin calibration curve used for total quantification. \begin{table}
\begin{tabular}{c c c c} \hline \hline  & \multicolumn{3}{c}{**Methanol Extract**} \\ \cline{2-4}  & \multicolumn{2}{c}{**TPC (mg GAE/g Extract)**} & \multicolumn{2}{c}{**TFC (mg RE/g Extract)**} \\ \cline{2-4} _Berberis_ & EMM & EMS & EMM & EMS \\ \cline{2-4} _Hispanic_ & 321.56 \(\pm\) 3.05 & 289.02 \(\pm\) 2.32 & 118.4 \(\pm\) 2.24 & 98.4 \(\pm\) 2.56 \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Total phenolic and flavonoid contents in _B. hispunica_ extracts. \begin{table}
\begin{tabular}{c c c c c} \hline \hline  & **RT** &
\begin{tabular}{c} **Molecular Weight** \\ **(g/mol)** \\ \end{tabular} & **Formula** & **Compound Name** & **Area (\%)** \\ \hline
1 & 3.80 & 100.16 & C\({}_{6}\)H\({}_{12}\)O & Hexanal & 4.542 \\ \hline
2 & 3.84 & 100.16 & C\({}_{6}\)H\({}_{12}\)O & 2-Hexen-1-ol, (E) & 4.542 \\ \hline
3 & 4.36 & 130.23 & C\({}_{8}\)H\({}_{18}\)O & 1-Heptanol, 3-methyl & 1.062 \\ \hline
4 & 4.86 & 394.5 & C\({}_{23}\)H\({}_{26}\)N\({}_{2}\)O\({}_{4}\) &
\begin{tabular}{c} N-(1-Hydroxy-4-oxo-1-phenylperhydroquinolizin-3-yl) \\ carbamic acid, benzyl ester \\ \end{tabular} & 1.075 \\ \hline
5 & 5.78 & 130.23 & C\({}_{8}\)H\({}_{18}\)O & 3.4-Dimethyl-2-hexano & 0.683 \\ \hline
6 & 6.26 & 112.17 & C\({}_{7}\)H\({}_{12}\)O & 2-Heptrenal, (Z) & 2.608 \\ \hline
7 & 6.38 & 414.4 & C\({}_{19}\)H\({}_{14}\)F\({}_{4}\)O\({}_{4}\)S &
\begin{tabular}{c} Benzoic acid 3-methyl-4-(1,3,3,3-tetrafluoro-2- \\ methoxycarbonyl-propenylsulfonyl)-phenyl \\ ester \\ \end{tabular} & 0.250 \\ \hline
8 & 6.89 & 184.36 & C\({}_{13}\)H\({}_{28}\) & 2,5,6-Trimethyldecane & 0.344 \\ \hline
9 & 7.79 & 142.2 & C\({}_{7}\)H\({}_{15}\)ClN\({}_{2}\)O & N-Methyl-3-piperidinecarboxamide & 0.250 \\ \hline
10 & 8.43 & 210.4 & C\({}_{15}\)H\({}_{30}\) & 2,4,6,8-Tetramethyl-1-undecene & 0.552 \\ \hline
11 & 9.17 & 186.33 & C\({}_{12}\)H\({}_{26}\)O & 1-Octanol, 2-butyl- & 0.552 \\ \hline
12 & 9.86 & 170.33 & C\({}_{12}\)H\({}_{26}\) & Dodecane & 0.698 \\ \hline
13 & 10.0 & 166.26 & C\({}_{11}\)H\({}_{18}\)O &
\begin{tabular}{c} 1-Oxspiro \\ [2.2]pentane,5-isopropylidene-2,2,4,4-tetramethyl \\ \end{tabular} & 0.344 \\ \hline
14 & 10.77 & 154.25 & C\({}_{10}\)H\({}_{18}\)O & 2-Decenal, (Z) & 0.438 \\ \hline
15 & 11.21 & 152.23 & C\({}_{10}\)H\({}_{16}\)O & 2,4-Decadiene & 2.340 \\ \hline
16 & 11.50 & 152.23 & C\({}_{10}\)H\({}_{16}\)O & 2,4-Decadiene & 3.786 \\ \hline
17 & 11.67 & 138.21 & C\({}_{9}\)H\({}_{14}\)O & 2,4-Nonadienal & 3.786 \\ \hline
18 & 12.46 & 296.6 & C\({}_{21}\)H\({}_{44}\) & Heptadecane,2,6,10,14-tetramethyl- & 1.290 \\ \hline
19 & 12.51 & 268.5 & C\({}_{19}\)H\({}_{40}\) & 2,3-Dimethylheptadecane & 1.290 \\ \hline
20 & 13.35 & 198.39 & C\({}_{14}\)H\({}_{30}\) & Tetradecane & 1.290 \\ \hline
21 & 14.10 & 224.42 & C\({}_{16}\)H\({}_{32}\) & 7-Hexadecene, (Z) & 0.276 \\ \hline
22 & 14.77 & 226.41 & C\({}_{16}\)H\({}_{34}\) & Hexadecane & 1.266 \\ \hline
23 & 14.84 & 184.37 & C\({}_{13}\)H\({}_{28}\) & Tridecane & 1.266 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Chemical compounds identified in the methanol extract of _B. hispanica_ bark. Figure 3: Chromatogram of the characterized extract from _B. hispanica_.