# Experimental Evolution of Magnetic Nanoparticle Resistance in _Escherichia coli_
## 3 Results
### Whole Genome
\begin{table}
\begin{tabular}{c c c c c c c} \hline  & & **(a)** & & & & \\ \hline
**Gene** & **Mutation** & **Annotation** & **FeNp1** & **FeNp2** & **FeNp3** & **FeNp4** & **FeNp5** \\ \hline rpoC\(\rightarrow\) & A\(\rightarrow\)T & D410V (GAT\(\rightarrow\)GTT) & 0.000 & 0.000 & 0.000 & 0.000 & 1.000 \\ rpoC\(\rightarrow\) & A\(\rightarrow\)G & D622G (GAC\(\rightarrow\)GGC) & 1.000 & 1.000 & 0.000 & 0.000 & 0.000 \\ \hline
**Gene** & **Mutation** & **Annotation** & **FeNp1** & **FeNp2** & **FeNp3** & **FeNp4** & **FeNp5** \\ \hline gloB\(\leftarrow\) & T\(\rightarrow\)A & E239V (GAG\(\rightarrow\)GTG) & 0.000 & 0.277 & 0.000 & 0.000 & 0.000 \\ \hline flgE\(\rightarrow\) & C\(\rightarrow\)T & A28A (GCC\(\rightarrow\)GCT) & 0.000 & 0.000 & 0.000 & 0.295 & 0.000 \\ nuoG\(\leftarrow\) & C\(\rightarrow\)T & G792S (GGT\(\rightarrow\)AGT) & 0.000 & 0.000 & 0.000 & 0.439 & 0.000 \\ \hline yfeX\(\leftarrow\) & G\(\rightarrow\)T & L263M (CTG\(\rightarrow\)ATG) & 0.251 & 0.000 & 0.000 & 0.000 & 0.000 \\ \hline ygiP\(\rightarrow\) & A\(\rightarrow\)G & Q17R (CAG\(\rightarrow\)CGG) & 0.270 & 0.000 & 0.000 & 0.000 & 0.000 \\ \hline rng\(\leftarrow\) & T\(\rightarrow\)A & H76L (CAC\(\rightarrow\)CTC) & 0.000 & 0.000 & 0.000 & 0.250 & 0.000 \\ \hline rpoA\(\leftarrow\) & C\(\rightarrow\)A & D199Y (GAC\(\rightarrow\)TAC) & 0.000 & 0.000 & 0.503 & 0.000 & 0.000 \\ \hline rpoA\(\leftarrow\) & G\(\rightarrow\)A & R191C (CGT\(\rightarrow\)TGT) & 0.000 & 0.000 & 0.000 & 0.000 & 0.665 \\ \hline chrR\(\rightarrow\) & G\(\rightarrow\)A & P32P (CCG\(\rightarrow\)CCA) & 0.000 & 0.000 & 0.258 & 0.000 & 0.000 \\ \hline gltP\(\rightarrow\) & A\(\rightarrow\)G & G250G (GGA\(\rightarrow\)GGG) & 0.000 & 0.302 & 0.000 & 0.000 & 0.000 \\ \hline
**(c)** & & & & & & & \\ \hline
**Gene** & **Mutation** & **Annotation** & **FeNp1** & **FeNp2** & **FeNp3** & **FeNp4** & **FeNp5** \\ \hline yagL\(\leftarrow\) & T\(\rightarrow\)A & E93V (GAG\(\rightarrow\)GTG) & 0.000 & 0.165 & 0.000 & 0.000 & 0.000 \\ \hline gapC\(\leftarrow\) & C\(\rightarrow\)T & pseudogene (254/750 nt) & 0.000 & 0.000 & 0.134 & 0.000 & 0.000 \\ \hline lsrD\(\rightarrow\) & A\(\rightarrow\)T & N258I (AAT\(\rightarrow\)ATT) & 0.000 & 0.000 & 0.159 & 0.000 & 0.000 \\ \hline pta\(\rightarrow\) & C\(\rightarrow\)T & R669C (CGT\(\rightarrow\)TGT) & 0.000 & 0.207 & 0.000 & 0.000 & 0.000 \\ \hline intS\(\rightarrow\) & G\(\rightarrow\)A & G77S (GGC\(\rightarrow\)AGC) & 0.000 & 0.175 & 0.000 & 0.000 & 0.000 \\ \hline yraj\(\rightarrow\) & A\(\rightarrow\)G & K85E (AAG\(\rightarrow\)GAG) & 0.154 & 0.000 & 0.000 & 0.000 & 0.000 \\ \hline prmA/dusB & A\(\rightarrow\)T & intergenic (+317/-12) & 0.000 & 0.000 & 0.000 & 0.000 & 0.209 \\ \hline hdeB\(\leftarrow\) & A\(\rightarrow\)T & N31K (AAT\(\rightarrow\)AAA) & 0.000 & 0.000 & 0.000 & 0.000 & 0.155 \\ \hline viaA\(\leftarrow\) & T\(\rightarrow\)A & E153V (GAA\(\rightarrow\)GTA) & 0.000 & 0.000 & 0.161 & 0.000 & 0.000 \\ \hline frwA\(\leftarrow\) & C\(\rightarrow\)T & E363E (GAG\(\rightarrow\)GAA) & 0.000 & 0.000 & 0.183 & 0.000 & 0.000 \\ \hline treR\(\leftarrow\) & C\(\rightarrow\)T & P275P (CCG\(\rightarrow\)CCA) & 0.000 & 0.000 & 0.000 & 0.000 & 0.111 \\ \hline sgCX\(\leftarrow\) & C\(\rightarrow\)A & Q155H (CAG\(\rightarrow\)CAT) & 0.152 & 0.000 & 0.000 & 0.000 & 0.000 \\ \hline \end{tabular}
\end{table} 
Table 3: **(a)** Selective sweeps in FeNP-resistant populations at day 25. **(b)** Significant polymorphisms in FeNP-resistant populations at day 25. **(c)** Minor polymorphisms in FeNP-resistant populations at day 25.