# Influence of Tomato Pomace on the Quality of Canned Pork Meat with Reduced Nitrogen Compounds
## 3 Results
### Results of Microbiological Analysis  
The number of CB varied from 2.283 in DFS C to 2.536 log CFU/g in DFS 0.5%. The TVC varied from 2.615 in DFS 0.5% to 2.728 log CFU/g in DFS C, while the count of LAB in the samples ranged from 1.405 in DFS 0.5% to 1.528 log CFU/g in DFS 2.5% (Table 4).  
A total of 81 isolates were identified using mass spectrometry (Figure 2). Four species, three genera, and three families were identified in the control samples of canned pork. The _Yersiniaceae_ and _Enterobacteriaceae_ families were the most commonly isolated. _Enterobacter cloacae_ and _Serratia liquefaciens_ were the most abundant species (35%), followed by _Enterococcus faecalis_ (18%) and _Enterobacter hormaechei_ (12%).  
Using mass spectrometry, fifty-six isolates (Figure 3) comprising three families, three genera, and four species were identified from the canned pork samples with the addition of 0.5% TPSP. _S. liquefaciens_ (35%) and _E. cloacae_ (29%) were the most commonly isolated species from the canned pork samples with an addition of 0.5% TPSP.  
Mass spectrometry was used to identify 63 isolates in total (Figure 4). In the canned pork samples with the addition of 1.5% of TPSP, a total of three species, three genera, and three families were isolated. The most frequently isolated families were _Enterobacteriaceae_ and _Yersiniaceae_. _E. cloacae_ was the most abundant species (44%), followed by _S. liquefaciens_ (40%) and _E. faecalis_ (16%).  
Using mass spectrometry, seventy isolates with high scores (Figure 5) and three families, three genera, and three species were identified from the canned pork samples with an addition of 2.5% TPSP. _S. liquefaciens_ (43%) and _E. cloacae_ (36%) were the most commonly isolated species from the canned pork samples with an addition of 2.5% TPSP.  
\begin{table}
\begin{tabular}{c c c c c} \hline
**Parameter** & **DFS C** & **DFS 0.5\%** & **DFS 1.5\%** & **DFS 2.5\%** \\ \hline CB–coliforms bacteria & 2.283 \(\pm\) 0.419 & 2.536 \(\pm\) 0.105 & 2.458 \(\pm\) 0.124 & 2.341 \(\pm\) 0.118 \\ TVC—total viable count & 2.728 \(\pm\) 0.154 & 2.616 \(\pm\) 0.209 & 2.671 \(\pm\) 0.191 & 2.615 \(\pm\) 0.104 \\ LAB–lactic acid bacteria & 1.506 \(\pm\) 0.112 & 1.405 \(\pm\) 0.089 & 1.470 \(\pm\) 0.146 & 1.528 \(\pm\) 0.131 \\ \hline DFS C—control sample; DFS 0.5\%—sample with 0.5\% addition of TPSP; DFS 1.5\%—sample with 1.5\% addition of TPSP; DFS 2.5\%—sample with 2.5\% addition of TPSP. No statistically significant differences were found between the samples (_p_\(\geq\) 0.05). & & \\ \end{tabular}
\end{table}
Table 4: Microbiological quality of canned pork samples. Values are means \(\pm\) standard deviation (n = 3).  
\begin{table}
\begin{tabular}{c c c c c} \hline
**Parameter** & **DFS C** & **DFS 0.5\%** & **DFS 1.5\%** & **DFS 2.5\%** \\ \hline ABTS [mg Trolox eqv. g\({}^{-1}\)] & 0.008 \(\pm\) 0.001 \({}^{\text{a}}\) & 0.011 \(\pm\) 0.001 \({}^{\text{b}}\) & 0.040 \(\pm\) 0.005 \({}^{\text{c}}\) & 0.048 \(\pm\) 0.003 \({}^{\text{d}}\) \\ DPPH [mg Trolox eqv. g\({}^{-1}\)] & 0.047 \(\pm\) 0.004 \({}^{\text{a}}\) & 0.067 \(\pm\) 0.004 \({}^{\text{b}}\) & 0.080 \(\pm\) 0.005 \({}^{\text{c}}\) & 0.090 \(\pm\) 0.002 \({}^{\text{d}}\) \\ TBARS [mg MDA kg\({}^{-1}\)] & 0.047 \(\pm\) 0.016 \({}^{\text{a}}\) & 0.084 \(\pm\) 0.029 \({}^{\text{a}}\) & 0.204 \(\pm\) 0.041 \({}^{\text{b}}\) & 0.256 \(\pm\) 0.034 \({}^{\text{b}}\) \\ \hline DFS C—control sample; DFS 0.5\%—sample with 0.5\% addition of TPSP; DFS 1.5\%—sample with 1.5\% addition of TPSP; DFS 2.5\%—sample with 2.5\% addition of TPSP. Means with different lowercase letters (a–d) differ significantly (_p_\(\leq\) 0.05). & & \\ \end{tabular}
\end{table}
Table 3: Antioxidant activity and oxidative stability of canned pork. Values are means \(\pm\) standard deviation (n = 3).  
Figure 3: Krona chart: isolated species, genera and family from samples with addition of 0.5% TPSP (DFS 0.5%).  
Figure 2: Krona chart: isolated species, genera and family from control samples (DFS C).