## 4 Discussion  
Chlorogenic acid and its isomers represent \(\sim\)90% of total phenylpropanoids in potatoes [19; 20]. The increase in phenolic compounds in potatoes induced by wounding stress has previously been reported [8; 9; 10; 19; 20]. For instance, [PERSON] et al. [19] reported a 3-4-fold increase in the total phenolic compound content of potato slices after 8 days of storage at 25 \({}^{\circ}\)C. Similarly, [PERSON] and [PERSON] [21] found an increase of 60% in total phenolics in purple-flesh potato slices stored at 15 \({}^{\circ}\)C for 48 h. Likewise, [PERSON] et al. [8] reported an increment in total phenolics by 100% and 60% in slices and pie-cuts, respectively, after 96 h of storage at 10 \({}^{\circ}\)C. However, to the best of our knowledge, this is the first report in the literature comparing the effect of storage temperature (10 \({}^{\circ}\)C and 20 \({}^{\circ}\)C) on the accumulation of CGA isomers in potato tubers. This is particularly relevant when potatoes are intended to be used as biofactories for antioxidant phenolic compounds. In potatoes, phenolic compounds biosynthesized during the wound-healing process are utilized for the biosynthesis of suberin and lignin [11; 13; 22; 23]. These polymers are produced as a defense mechanism to prevent water loss in wounded plant tissues [13; 15; 19; 22; 23; 24]. The biosynthesis of both polymers starts at the phenylpropanoid metabolism, and it is well known that this is induced by wounding stress in plants [16; 25]. This pathway involves enzymes such as the phenylalanine ammonia lyase (PAL), cinnamyl alcohol dehydrogenase (CAD), and peroxidase (POD), which are involved in lignin and suberin biosynthesis [25]. The activation of the phenylpropanoid metabolism by wounding in potatoes has been previously documented by an increase in PAL activity [21; 26; 27]. Likewise, [PERSON] et al. [28] studied the change in the activities of enzymes related to lignification enzymes in loquat fruit flesh stored at different temperatures after harvest. The authors reported higher enzymatic activities at 20 \({}^{\circ}\)C when compared with 12 \({}^{\circ}\)C after 8 days of storage, suggesting that the difference in the CGAs profiles detected between pie-cuts stored at 10 \({}^{\circ}\)C and 20 \({}^{\circ}\)C can be attributed to the effect of temperature on enzymes involved in CGA production, and their utilization for suberin and lignin biosynthesis. In agreement with previous reports, the major phenolic compound accumulated in wounded potatoes was CGA [8; 9; 10]. Although the specific route of the biosynthesis of CGA in potatoes has not been elucidated, the supposed biosynthetic routes involve hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) and/or hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT) [17]. HQT catalyzes CGA synthesis in potatoes [29], whereas HCT is involved in CGA mobilization toward suberin [17]. Our results suggested that during the first 96 h of storage of the wounded potatoes, the balance between the individual CGA biosynthesis rate and the utilization rate for suberin and lignin production differ, affecting the CGA profile of the tissue. However, to better understand this phenomenon, it would be needed to determine the enzymatic activity of HQT and HCT to elucidate how the accumulation of specific CGAs is favored depending on the storage temperature selected for the wounded tissue. Likewise, it is important to point out that the specific role of CGA isomers (neo-CGA and crypto-CGA) as precursors for the biosynthesis of lignin and suberin remains unknown [30], and their involvement as precursors for the biosynthesis of lignin has not been elucidated. Indeed, the biosynthesis pathway of chlorogenic acids isomers (neo-CGA and crypto-CGA) also remains unknown [30].