# Bioremediation of Persistent Organic Pollutant--Oxybenzene with _Pleurotus diamor_
## 3 Results
### Identification of Biodegradation Products of Oxybenzene and Toxicity Evaluation
\begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Retention** & **Symbol in** & **Summary Formula** & **Daughter Ions [_m/z_]; Formula** \\
**Time (t\({}_{\text{r}}\)) (min)** & **Figure** & **[M+H\({}^{+}\)]** & **Daughter Ions [_m/z_]; Formula** \\ \hline
2.52 & A & C\({}_{16}\)H\({}_{17}\)O\({}_{4}\)S & 287.0738; C\({}_{16}\)H\({}_{15}\)O\({}_{3}\)S & \\
2.52 & A & C\({}_{16}\)H\({}_{17}\)O\({}_{4}\)S & 269.0633; C\({}_{16}\)H\({}_{13}\)O\({}_{2}\)S & \\  & & & 241.0684; C\({}_{15}\)H\({}_{13}\)OS & \\ \hline
2.65 & B & C\({}_{16}\)H\({}_{17}\)O\({}_{4}\)S & 255.0478; C\({}_{15}\)H\({}_{11}\)O\({}_{2}\)S & \\
2.65 & B & C\({}_{16}\)H\({}_{17}\)O\({}_{4}\)S & 227.052; C\({}_{14}\)H\({}_{11}\)OS & \\  & & & 211.0576; C\({}_{14}\)H\({}_{11}\)S & \\  & & & 209; C\({}_{14}\)H\({}_{9}\)S & \\ \hline
2.89 & C & C\({}_{15}\)H\({}_{11}\)O\({}_{4}\) & 237.0552; C\({}_{15}\)H\({}_{9}\)O\({}_{3}\) & \\  & & & 227.0707; C\({}_{14}\)H\({}_{11}\)O\({}_{3}\) & \\
2.89 & C & C\({}_{15}\)H\({}_{11}\)O\({}_{4}\) & 199.0758; C\({}_{13}\)H\({}_{11}\)O\({}_{2}\) & \\  & & & 181.0650; C\({}_{13}\)H\({}_{9}\)O & \\  & & & 137.0239; C\({}_{7}\)H\({}_{5}\)O\({}_{3}\) & \\ \hline
3.01 & D & C\({}_{16}\)H\({}_{13}\)O\({}_{5}\) & 270.0524; C\({}_{15}\)H\({}_{11}\)O\({}_{5}\) & \\  & & & 229.0863; C\({}_{14}\)H\({}_{13}\)O\({}_{3}\) & \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Detailed data of LC-MS/MS analysis of solutions after _Pleurotus jamor_ incubation. Figure 3: A representative chromatogram of the analyzed extracts of mycelium from in vitro cultures of _P. djamor_ grown with (**A**) and without (**B**) oxybenzene. Red letters (A–M) are explained in Table 3.  
Finally, the evaluation of toxicity risks associated with oxygenzone and its mycoremediation products was conducted for the biotransformation products of oxygenzone. Predictions were generated using the OSIRIS Property Explorer tool (accessed on 20 February 2025 from [[https://www.organic-chemistry.org/prog/peo/tox.html](https://www.organic-chemistry.org/prog/peo/tox.html)]([https://www.organic-chemistry.org/prog/peo/tox.html](https://www.organic-chemistry.org/prog/peo/tox.html))). The prediction of the mutagenic, tumorigenic, irritant, and reproductive effects of Products A-M was conducted (Table 4). According to OSIRIS, the highest toxicity risk was identified for degradants E and L. No toxicity risk was indicated for products D, H, and M. \begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Compound** & **Mutagenic** & **Tumorigenic** & **Irritant** & **Reproductive Effects** \\ \hline \multirow{2}{*}{Oxygenzone} & known to be & known to be & & high-risk \\  & mutagenic & tumorigenic & – & fragment \\ \hline \multirow{2}{*}{Product A} & medium-risk & \multirow{2}{*}{–} & \multirow{2}{*}{–} & \multirow{2}{*}{–} \\  & fragment & & & \\ \hline \multirow{2}{*}{Product B} & – & – & medium-risk & \\  & & – & fragment & – \\ \hline \multirow{2}{*}{Product C} & – & – & medium-risk & \\  & mutagenic & & fragment & – \\ \hline \multirow{2}{*}{Product A} & medium-risk & \multirow{2}{*}{–} & \multirow{2}{*}{–} & \multirow{2}{*}{–} \\  & fragment & & & \\ \hline \multirow{2}{*}{Product B} & high-risk & medium-risk & high-risk & medium-risk \\  & fragment & fragment & fragment & fragment \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Toxicity risk assessment of oxygenzone and its biodegradation products using _Pleurotus__janor_ mycelium generated by OSIRIS Property Explorer.