## 2 Materials and Methods
### Determination of Biogenic Amine Content  
Identification and quantification of biogenic amines were carried out by high-performance liquid chromatography (HPLC). For the extraction, 5 g of sample was homogenized with 20 mLof (0.4 mol/L) perchloric acid solution (HClO4, Chempur, Karlsruhe, Germany) into a 50 mL screw-cap tube and 250 uL internal standard (1,7-diminoheptane, C?H18N2, Sigma-Aldrich(r)) stock solution (1 mg/mL) added to achieve 1 \(\upmu\)g/mL concentration level in injection volume. After that, the mixture was centrifuged (Hermle Z 306, Gosheim, Germany) at 4000 rpm and supernatant rinsed into a 25 mL bottle through Whatman no. 1 filter paper (180 \(\upmu\)m thickness and 11 \(\upmu\)m particle retention rating at 98% efficiency). Filtrate was adjusted to 25 mL with perchloric acid solution (0.4 mol/L).  
For the derivatization, 500 uL sample extract alkaline by adding 100 uL sodium hydroxide (NaOH, Eurochemicals, Kuprioniskes, Lithuania) solution (2 mol/L) and buffer sample by adding 150 uL saturated sodium bicarbonate (NaHCO3, Lachema, Brno, Czech Republic). Add 1 mL dansyl chloride (5-dimethylaminobenz-1-sulfonyl chloride, Sigma-Aldrich(r) solution (10 mg/mL), mix thoroughly with shaker mixer (Reax Top, Heidolph Instruments GmbH & Co. KG, Schwabach, Germany), and transfer reaction mixture to 40 \({}^{\circ}\)C incubator for 45 min. After incubation, cool to room temperature for 10 min and remove residual dansyl chloride by adding 50 uL ammonia (25%, NH3, Chempur, Germany). Mix with shaker mixer. After 30 min, adjust to 5 mL with ammonium acetate (0.1 mol/L, CH3 COONH4, Reachem, Petrzalka, Slovakia):acetonitrile ([PERSON], Val-de-Reuil, France) mixture (1:1, \(v/v\)) and mix well with shaker mixer. Filter through 0.20 \(\upmu\)m nylon filter (UptiDisc, Interchim, Montlucon, France) and inject the solution into the analytical column.  
A Shimadzu Prominence LC20 AD (Shimadzu, Japan) coupled with a UV detector SPD/20 A chromatographic system was used with a LabSolution (Shimadzu, Japan) integrator using a Hydrosphere C18 (5 \(\upmu\)m, 12 mm), 150 \(\times\) 4.6 I.D. (YMC Co., Ltd., Kyoto, Japan) column and YMC precolumn (YMC Co., Ltd.) ProcC18 (10 \(\times\) 3.0 mm L.D., S-3 \(\upmu\)m, 12 nm). LC mobile phase A: ammonium acetate (0.1 mol/L), phase B: acetonitrile. Operating conditions: flow rate 0.9 mL/min; injection volume 20 \(\upmu\)L; column temperature 40 \({}^{\circ}\)C; peaks detected at 254 nm; gradient 50% B to 90% B in 19 min; run time 20 min; post-run before next run, 50% B, 8 min.  
Histamine (C5H9N3) biogenic amine standard was purchased from Sigma-Aldrich(r). Stock solutions (1 mg/mL) of amine were prepared in 0.1 N HCI (Stanlab, Lublin, Poland) and stored at 4 \(\pm\) 1 \({}^{\circ}\)C. For amine identification, the standard solution of individual biogenic amine was chromatographed separately and mixed to determine the retention times and the response. Standard curves with correlation coefficients for stock solutions were obtained by the external standard method. All the results were expressed in milligrams per kilogram. Histamine was quantified using the HPLC method outlined in ISO 19343:2017.