## 3 Results
lucidum_ spores is shown in Figure 4.  
Figure 3: Total ion chromatograms (TICs) profiles of FAMEs obtained after transesterification of triglycerides contained in samples of _Ganoderma lucidum_ extracted with different processes: (**A**) extraction with \(n\)-hexane; (**B**) extraction in Soxhlet apparatus with \(n\)-hexane; (**C**) reflux extraction with chloroform; (**D**) commercial oil. Table 2 shows complete lipid profiles corresponding to the fatty acid chains of triglycerides in the form of percentage concentrations and the corresponding standard deviations (SD). Reported SDs were evaluated by first calculating the standard deviation of each target analyte concentration by using the standard deviation \(s_{y/x}\) of the signal about the regression line. \(s_{y/x}\) is \begin{table}
\begin{tabular}{c c c c c c} \hline  & \multicolumn{2}{c}{**Extraction with**} & \multicolumn{2}{c}{**Extraction in**} & \multicolumn{2}{c}{**Reflux Extraction with**} & \multicolumn{2}{c}{**Commercial Liquid**} \\  & \multicolumn{2}{c}{_n_-Hexane (\% \(\pm\) SD 2)**} & **Soxhlet (\% \(\pm\) SD)** & **Chlororform (\% \(\pm\) SD)** & **Extract (\% \(\pm\) SD)** \\ \hline C14:0 & Myristic acid & \(0.14\pm 0.03\) & \(0.24\pm 0.06\) & \(0.14\pm 0.03\) & \(0.09\pm 0.04\) \\ \hline C15:0 & – & \(0.55\pm 0.03\) & \(1.01\pm 0.05\) & \(0.61\pm 0.03\) & \(0.51\pm 0.05\) \\ \hline C16:1n-7 & Palmitoleic acid & \(1.4\pm 0.15\) & \(5.83\pm 0.07\) & \(4.3\pm 0.4\) & \(2.3\pm 0.3\) \\ \hline C16:0 & Palmitic acid & \(18.2\pm 0.15\) & \(21.3\pm 0.24\) & \(17.7\pm 0.21\) & \(16.3\pm 0.2\) \\ \hline C17:0 & Margaric acid & \(0.55\pm 0.02\) & \(0.80\pm 0.03\) & \(0.39\pm 0.02\) & \(0.33\pm 0.03\) \\ \hline C18:2n-6 & linoleic acid & \(3.19\pm 0.03\) & \(3.00\pm 0.03\) & \(2.68\pm 0.03\) & \(9.5\pm 0.10\) \\ \hline C18:1n-9 & Oleic acid & \(68.4\pm 0.97\) & \(60.1\pm 0.84\) & \(66.9\pm 0.90\) & \(65.4\pm 0.91\) \\ \hline C18:0 & Stearic acid & \(5.77\pm 0.03\) & \(5.94\pm 0.04\) & \(5.52\pm 0.04\) & \(4.38\pm 0.03\) \\ \hline C20:1n-9 & Gadoleic acid & \(0.52\pm 0.02\) & \(0.61\pm 0.03\) & \(0.54\pm 0.02\) & \(0.45\pm 0.03\) \\ \hline C20:0 & Arachid acid & \(0.45\pm 0.02\) & \(0.56\pm 0.05\) & \(0.50\pm 0.03\) & \(0.42\pm 0.04\) \\ \hline C22:0 & Behenic acid & \(0.48\pm 0.02\) & \(0.41\pm 0.04\) & \(0.47\pm 0.02\) & \(0.28\pm 0.03\) \\ \hline C24:0 & Lignoerci acid & \(0.30\pm 0.02\) & \(0.11\pm 0.03\) & \(0.32\pm 0.01\) & \(0.09\pm 0.03\) \\ \hline \end{tabular}
\end{table} 
Table 2: Fatty acids composition (percentage of FAMEs) contained in samples of _Gnaoderma lucidum_ obtained with different extraction processes. Figure 4: Representative annotated total ion chromatogram (TIC) of FAMEs in samples of _Gnaoderma lucidum_ spores. a statistic which is commonly called \"residual deviation about the regression\" and which can be readily obtained from calibration data under the assumption of homoscedastic data [26]. The standard deviations on analyte concentrations are finally converted to the reported standard deviations on percentage by a standard uncertainty propagation formula. All analyzed samples contain a significant amount of oleic (C18:In-9) and palmitic (C16:0) acids in the range from 60.1% to 68.4% and from 16.3% to 21.3%, respectively. In the examined samples, stearic (C18:0), palmitoleic (C16:In-7) and linoleic (C18:2n-6) acids are present in much lower amounts (<10%). In addition, a small amount of lignoercic (C24:0), myristic (C14:0) and arachidonic (C20:0) acids were detected in all the samples (<1%). Interestingly, our findings show that the commercially available liquid extract and the extracts of spores of _G. lucidum_ produced in this study employing different processes, have similar lipid contents as revealed by NMR and GC-MS data. Comparing the processes adopted to extract the spores (Figure 5), some interesting results can be observed. The extract of spores of _G.