## 3 Results and Discussion
### Extracts' Characterization by HPLC
#### 3.4.2 Polyphenolic Composition  
For the estimation of PEF effects in the polyphenolic profile of SOLL, extracts in the optimum EtOH-H\({}_{2}\)O ratio (25% EtOH) were selected. The main compounds of SOLL \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Compound** & **RT 1 (min)** & **Reference** & \
\begin{tabular}{c} **PEF Treated** \\ **(100 us)** \\ \end{tabular} & **Compound** & **RT (min)** & **Reference** &
\begin{tabular}{c} **PEF Treated** \\ **(100 us)** \\ \end{tabular} \\ \hline _trans_, _trans_-2.4-Hexaelian & 7.798 & 0.17 & 0.33 & Piperitenne & 34.651 & nd & 0.09 \\ Benzaldehyde & 9.907 & 0.05 & Nd 2 & Eugenol & 36.322 & 0.02 & 0.06 \\ Sabineine & 12.190 & 0.03 & nd & 3-Czene & 36.648 & 0.02 & nd \\
1-Octen-3-ol & 12.548 & 0.31 & nd & \(\alpha\)-Cubebene & 37.305 & 0.12 & 0.10 \\
8-Pinene & 13.570 & 0.18 & nd & L-Bornol acetate & 38.244 & 0.04 & nd \\
8-Crippinene & 14.918 & 0.04 & 0.16 & \(\upmu\)-Cubebene & 39.580 & 0.04 & 0.03 \\ \(p\)-Cymene & 15.099 & 0.16 & 0.30 & \(\alpha\)-Cubebene & 39.980 & 0.01 & nd \\ Eucalyptus & 15.777 & 10.67 3 & 3.35 & \(\alpha\)-Gurjunene & 40.753 & 0.09 & nd \\ \(\gamma\)-Terpinene & 17.686 & 0.30 & 0.23 & Caryophyllene & 41.089 & 0.56 & 0.06 \\ _trans_-4-Thujanol & 18.130 & 0.41 & 0.31 & Aromandendrene & 42.257 & 0.07 & nd \\
2-Cymene & 19.233 & 0.07 & 0.06 & \(\upmu\)-Cadienne & 42.895 & 0.03 & 0.06 \\
2-Czene & 19.621 & 0.11 & nd & Humulene & 43.034 & 0.11 & nd \\ \(\alpha\)-Thujome & 20.110 & 3.02 & 1.44 & \(\upmu\)-Copane & 43.577 & 0.04 & 0.04 \\
9-Thujome & 21.139 & 11.07 & 4.10 & \(\upmu\)-Cadienne & 44.452 & 0.02 & nd \\ D-Campphor & 22.397 & 1.05 & 19.82 & Germarche D & 44.461 & 0.15 & 0.21 \\ DL-Campphor & 22.437 & 1.38 & nd & \(\gamma\)-Cadienne & 44.952 & 0.08 & 0.07 \\ L-Campphor & 22.611 & 8.23 & nd & \(\alpha\)-Cadienne & 45.147 & 0.07 & 0.17 \\
1-Campheme & 22.689 & 0.47 & nd & \(\upmu\)-Elemene & 45.574 & 0.07 & nd \\
1-Camphene & 23.100 & 0.71 & nd & Epizonarene & 45.669 & 0.08 & 0.13 \\ D-Pinemphone & 23.388 & 0.07 & nd & \(\upmu\)-Muurolene & 46.088 & 0.10 & 0.18 \\ Isoborneol & 23.666 & 0.17 & nd & \(\upmu\)-Muurolene & 46.665 & 0.19 & 0.22 \\
2-Bromanol & 24.350 & 7.00 & 2.33 & \(\upmu\)-Caliamenene & 46.822 & 0.07 & 0.06 \\ Bornol & 24.522 & 3.91 & 9.56 & \(\upmu\)-Clemene & 47.050 & 0.74 & 1.26 \\ L-Borneol & 24.898 & 10.65 & 26.35 & \(\upmu\)-Santelene & 47.216 & nd & 0.81 \\ Bornol & 24.970 & 3.43 & nd & \(\upmu\)-Cadienne & 47.413 & 0.30 & 0.41 \\ \(p\)-Cymen-8-ol & 4  & 25.325 & nd & 0.17 & 1.4-Cadiadiene & 47.774 & 0.03 & nd \\ Terpinene-4-ol & 25.426 & 1.21 & 1.55 & \(\upmu\)-Cadiarone & 47.890 & 0.03 & nd \\ \(\upmu\)-Carpene & 25.787 & 0.15 & 0.20 & \(\upmu\)-Cadienne & 48.106 & 0.02 & 0.06 \\ \(\upmu\)-Terpineol & 26.177 & 0.57 & 1.44 & \(\upmu\)-Cadienne & 49.721 & 0.07 & 0.12 \\ Myrfrench & 26.569 & 0.20 & 0.52 & Caryophyllene oxide & 49.28 & 0.18 & 0.11 \\ \(\upmu\)-trans-Pinocravel & 26.669 & nd & 0.10 & Diethyl phthalate & 50.049 & 0.10 & 0.08 \\ \(\upmu\)-Carveol & 27.927 & 0.05 & 0.10 & (+)-Y-Gurjunene & 50.714 & 0.38 & 0.36 \\ \(\upmu\)-Greenyl valerate & 29.787 & 0.07 & nd & \(\upmu\)-Cdauiane & 51.309 & 0.07 & nd \\ \(\upmu\)-2-Hexenyl valerate & 30.328 & 0.05 & nd & Calarene & 53.430 & nd & 0.19 \\ \(\upmu\)-Cimenez & 30.871 & 0.07 & 0.22 & (+)-Epizonarene & 53.683 & nd & 0.04 \\ \(\upmu\)-Oncenorph & 31.162 & 0.17 & 0.55 & \(\upmu\)-Suaine & 53.714 & 0.05 & nd \\ Bornyl acetate & 32.491 & 1.65 & 0.43 & Aliaromondendrene & 54.477 & 0.04 & nd \\ Sabiny acetate & 32.980 & 0.21 & 0.07 & (+)-Longfoliene & 54.716 & nd & 0.04 \\ Thymol & 33.322 & 0.11 & 0.23 & (-)-Y-Cadienne & 56.080 & nd & 0.51 \\ Carvacrol & 33.789 & 0.42 & 0.39 & (-)-\(\upmu\)-Amorphene & 56.134 & 0.41 & nd \\ \hline \hline \end{tabular} Reference extract Total = 87.97%.  
\end{table} 
Table 2: Percentage of volatile compounds determined in _Salvia officinalis_ L. extracts (in 25% EtOH) by HS-SPME/GC-MS.  
found in this work are in line with previous findings [42; 43].