# Different _Chondrus crispus_ Aquaculture Methods and Carrageenanan Extraction
## 3 Results  
### Water Physicochemical Parameters  
By analyzing the table regarding the physicochemical data (Tables 1 and S2-S8), it is possible to state that there are large variations in the parameters, mainly due to the time of the year when the measurements were taken. In general, the lowest pH was measured in the laboratory, as some days the outside tanks demonstrated a dangerously high pH (and therefore the floodgates were opened to change the tank water). As for water oxygenation, in the laboratory tanks the goal was to have 120% of Dissolved Oxygen (DO) as per the study of [PERSON] in 2005, but the outdoor tanks always showed a higher DO percentage (about 150) [50]. The total dissolved solids (TDS) were controllable in the laboratory, and since the outdoor tanks were dirt tanks, the TDS value was expected to be higher. The laboratory measurements were always close to 20 ppt TDS, while the Final Fixed Lines method (an outdoor method) presented a mean TDS value of 24.03 ppt TDS. This could have impaired algae growth, stopping photosynthesis due to the solids blocking the passage of light through the water. In terms of temperature, different values were observed mainly due to the seasonal shifts that occurred during the duration of the study. The Final Fixed Lines Method had the highest average temperature with a value of 24.34 \({}^{\circ}\)C. The lowest mean temperature was also in an outdoor tank, in the Cage method, with a value of 13.93 \({}^{\circ}\)C. The highest \begin{table}
\begin{tabular}{c c c c c c c c c c c c} \hline  & **mVpH** & **pH** & **mVORP** & **\%DO** & **ppm** & **mS/cm** & **mS/cm\({}^{\star}\)** & **ppt** & **PSU** & **ot** & \({}^{\circ}\)C** & **psi** \\ \hline \multirow{2}{*}{Nursery 1} & \(-73.05\) & 8.28 \(\pm\) & 132.35 & 119.88 & 11.31 \(\pm\) & 35.02 \(\pm\) & 26.30 \(\pm\) & 17.51 \(\pm\) & 22.05 \(\pm\) & 16.54 \(\pm\) & 11.94 & 14.78 \\  & \(\pm\) 13.66 & 0.25 & \(\pm\) 46.69 & \(\pm\)10.65 & 1.14 & 1.55 & 1.42 & 0.77 & 1.01 & 1.00 \(\pm\) & 2.47 \(\pm\) & 0.04 \\ \hline \multirow{2}{*}{Nursery 2} & \(-77.59\) & 8.35 \(\pm\) & 146.46 & 126.97 & 11.16 \(\pm\) & 39.48 \(\pm\) & 31.18 \(\pm\) & 19.74 \(\pm\) & 25.04 \(\pm\) & 18.66 \(\pm\) & 13.94 & 14.74 \\  & \(\pm\) 6.15 & 0.11 & \(\pm\) 37.53 & \(\pm\) 9.38 & 0.82 & 1.55 & 1.35 & 0.77 & 1.49 & 0.85 \(\pm\) & 0.79 \(\pm\) & 0.10 \\ \hline \multirow{2}{*}{Nursery 3} & \(-74.49\) & 8.28 \(\pm\) & 134.13 & 122.67 & 10.24 \(\pm\) & 38.99 \(\pm\) & 32.46 \(\pm\) & 19.49 \(\pm\) & 24.90 \(\pm\) & 17.96 \(\pm\) & 16.23 & 14.68 \\  & \(\pm\) 6.77 & 0.12 & \(\pm\) 47.40 & \(\pm\) 3.48 & 0.38 & 1.75 & 1.24 & 0.88 & 1.24 & 1.10 \(\pm\) & 1.10 \(\pm\) & 0.05 \\ \hline \multirow{2}{*}{Nursery 3} & \(-76.84\) & 8.30 \(\pm\) & 102.05 & 154.11 & 11.75 \(\pm\) & 40.03 \(\pm\) & 37.24 \(\pm\) & 20.01 \(\pm\) & 25.61 \(\pm\) & 17.24 \(\pm\) & 21.38 & 14.78 \\  & \(\pm\) 4.47 & 0.08 & \(\pm\) 28.88 & \(\pm\) 17.98 & 1.11 & 1.48 & 1.64 & 0.74 & 1.07 & 1.31 \(\pm\) & 2.58 \(\pm\) & 0.06 \\ \hline \multirow{2}{*}{Cages} & \(-93.30\) & 8.64 \(\pm\) & 87.14 \(\pm\) & 148.35 & 13.55 \(\pm\) & 33.57 \(\pm\) & 26.46 \(\pm\) & 16.79 \(\pm\) & 21.12 \(\pm\) & 15.48 \(\pm\) & 13.93 & 14.88 \\  & \(\pm\) 20.10 & 0.38 & 99.83 \(\pm\) & 27.93 & 2.55 & 3.50 & 2.51 & 1.75 & 2.41 \(\pm\) & 2.01 \(\pm\) & 1.75 \(\pm\) & 0.20 \\ \hline \multirow{2}{*}{Final  Fixed  Lines} & \(-90.42\) & 8.53 \(\pm\) & 97.94 \(\pm\) & 178.29 & 12.63 \(\pm\) & 48.05 \(\pm\) & 47.41 \(\pm\) & 24.03 \(\pm\) & 31.33 \(\pm\) & 20.77 \(\pm\) & 24.34 & 14.89 \\ \cline{1-1}  & \(\pm\) 7.67 & 0.13 & 43.79 \(\pm\) & 34.70 & 2.24 & 2.43 & 2.10 & 1.21 & 1.79 & 1.70 \(\pm\) & 1.83 \(\pm\) & 0.05 \\ \hline \end{tabular}
\end{table} 
Table 1: Mean physicochemical parameters of water in seaweed cultivation throughout the whole study (\(\pm\)Standard Deviation). For Nursery 1, and Nursery 3, \(n\) = 6. For Nursery 2, \(n\) = 2. For Nursery 3 in Tordos, Cage, and Final fixed lines, \(n\) = 1.  
mean salinity was observed in the Final Fixed Lines method, in which the value presented was 31.33 PSU.