###### Abstract  
_Dendrobium mobile_ Lindl., as an endangered medicinal plant within the genus _Dendrobium_, is widely distributed in southwestern China and has important ecological and economic value. There are a variety of metabolites with pharmacological activity in \(D\). _nobile_. The alkaloids and polysaccharides contained within \(D\). _nobile_ are very important active components, which mainly have antiviral, anti-tumor, and immunity improvement effects. However, the changes in the compounds and functional genes of \(D\). _nobile_ induced by methyl jasmonate (MeJA) are not clearly understood. In this study, the metabolome and transcriptome of \(D\). _nobile_ were analyzed after exposure to MeJA. A total of 377 differential metabolites were obtained through data analysis, of which 15 were related to polysaccharide pathways and 35 were related to terpenoids and alkaloids pathways. Additionally, the transcriptome sequencing results identified 3256 differentially expressed genes that were discovered in 11 groups. Compared with the control group, 1346 unigenes were differentially expressed in the samples treated with MeJA for 14 days (TF14). Moreover, the expression levels of differentially expressed genes were also significant at different growth and development stages. According to GO and KEGG annotations, 189 and 99 candidate genes were identified as being involved in terpenoid biosynthesis and polysaccharide biosynthesis, respectively. In addition, the co-expression analysis indicated that 238 and 313 transcription factors (TFs) may contribute to the regulation of terpenoid and polysaccharide biosynthesis, respectively. Through a heat map analysis, fourteen terpenoid synthetase genes, twenty-three cytochrome P450 oxidase genes, eight methyltransferase genes, and six aminotransferase genes were identified that may be related to dendrobine biosynthesis. Among them, one sesquiterpene synthase gene was found to be highly expressed after the treatment with MeJA and was positively correlated with the content of dendrobine. This study provides important and valuable metabolomics and transcriptomic information for the further understanding of \(D\). _nobile_ at the metabolic and molecular levels and provides candidate genes and possible intermediate compounds for the dendrobine biosynthesis pathway, which lays a certain foundation for further research on and application of _Dendrobium_. Dendrobium mobile Lindl. ; metabolome; transcriptome; terpenoids; dendrobine; sesquiterpene synthase 2023287892. 287892. Dendrobium, D. nobile_ is a fundamental plant for the production of \"Shi Hu\" according to _Chinese Pharmacopoeia_ (2020 edition). _D. nobile_ demonstrates the activity of tonifying Yin, nonurishing the stomach, benefiting essence, tonifying the kidneys, treating deficiencies of the five internal organs, and so on [2]. As an ornamental and medicinal orchid, _D. nobile_ is an important raw material in the development of various medicinal formulations, such as the Mailuoning injection, _Dendrobium_ Yeguang pills, and _Dendrobium_ Mingmu pills. The main components found in _D. nobile_ are flavonoids, alkaloids, phenols, polysaccharides, sesquiterpenoids, coumarins, and steroidal glycosides [3; 4; 5; 6; 7]. _D. nobile_ alkaloids (DNLA) are one of the prime active ingredients derived from _D. nobile_; they possess diverse pharmacological effects, such as forming an anti-white internal barrier, anti-tumor activity, blood sugar lowering, blood fat lowering, and nerve protection [8]. Moreover, the polysaccharides from _D. nobile_ (DNPs) show a variety of biological activities, such as anti-viral [9], anti-tumor [9], anti-oxidant [10], anti-photodamage [11], and immunomodulatory [12] effects, as well as stimulating follicular development improvements [4], and protection against gastric damage [13]. Additionally, _D. nobile_ extract also demonstrates the functions of whitening, moisturizing, anti-aging, and constipation reduction [14]. Hence, _D. nobile_ also has good application prospects in the cosmetics industry.