###### Abstract  
Agorforestry by-products have gained rising attention in recent years as they represent inexpensive and abundant raw materials that are a source of added-value chemicals, e.g., for food and pharmaceutical applications, as well as for bioenergy generation. _Pinus pinaster_ Ait. bark extracts are consumed worldwide for their cardiovascular benefits, whilst the health potential of _Pinus pinac_ L. bark has not yet been deeply exploited. Therefore, this study highlights the chemical characterisation of Portuguese _P. pinaster_ Ait. and _P. pinen_ L. bark polar extracts, via ultra-high performance liquid chromatography-diode array detection-tandem mass spectrometry (UHPLC-DAD-MS\({}^{n}\)) analysis, and their antioxidant and antibacterial activities. Quinic acid, an A-type proycapidin dimer isomer, protocatechic acid, and quercetin were identified for the first time as _P. pinen_ L. bark components. Moreover, this bark demonstrated a higher total content of identified polar compounds than _P. pinaster_ Ait. bark, with quinic acid being the most abundant compound identified. Regarding antioxidant activity, the pine bark polar extracts exhibited strong reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis-(3-ethyl-benzothazoline-6-sulfonic acid (ABTS) radical scavenging effects compared to natural antioxidants. Moreover, the bactericidal actions of pine bark extracts were shown against _Staphylococcus aureus_ and _Escherichia coli_ at a 3.13-25 mg mL\({}^{-1}\) range. Globally, these promising insights can boost the sustainable exploitation of _P. pinen_ L. bark, as already occurs with _P. pinaster_ Ait. bark, for the food and biomedical fields. [PERSON], [PERSON], [PERSON], [PERSON], S.  
forests (_ca._ 9.6 \(\times\) 10\({}^{5}\) ha of continental soil), after the _Quercus_ spp. family. The Portuguese pine forest mainly consists of _Pinus pinaster_ Ait. and _P. pinea_ L., which, in 2015, accounted for ca. 7.1 \(\times\) 10\({}^{5}\) and 1.9 \(\times\) 10\({}^{5}\) ha of the continental soil, respectively [3]. Given their good adaptation to edaphoclimatic conditions, notably frost resistance, adaptation to summer drought, and tolerance to poor soils, _P. pinaster_ Ait. has been planted for coastal sand dunes stabilisation, soil conservation, and agricultural culture protection against salt spray. Additionally, _P. pinaster_ Ait. wood plays a crucial role in the national economy as it is used for construction, furniture, panels, pulp fibers, and energy generation, whereas the resin is primarily intended for manufacturing oils, varnishes, and adhesives [4; 5]. In a similar way, _P. pinea_ L. is also well-adapted to the coastal Mediterranean climate and thrives preferentially in siliceous and sandy soils. Thus, _P. pinea_ L. has been also cultivated in sand dunes for coastal area preservation. Furthermore, the edible seeds or nuts are considered to be the most economically valuable product of the _P. pinea_ L. forest due to their unique taste and also their interesting nutritional composition in terms of proteins, fats, ascorbic acid, thiamine, and riboflavin [6]. Both the pine wood and the seed industries generate considerable quantities of byproducts, mainly of the _P. pinaster_ Ait. and _P. pinea_ L. barks, accounting, in 2015, for 5000 and 600 t in Portugal, respectively [3]. _Pinus_ spp. bark has been used for energy generation, but it can be valorised as a renewable and low-cost source of biologically active compounds for food, nutraceutical, and pharmaceutical applications [7]. Actually, Pycnogenol\({}^{\circledR}\), Flavangenol\({}^{\circledR}\), and Oligopin\({}^{\circledR}\) are commercially available extracts of _P. pinaster_ Ait. bark, which contains phenolic compounds, namely procyanidins, phenolic acids (e.g., protocatechuic, gallic, ferulic, and caffeic acids), and flavanones (e.g., taxifolin and taxifolin-3\({}^{\prime}\)-\(\beta\)-D-glucose) [7; 8; 9]. These commercial extracts have been associated with diverse health benefits.