## 3 Results and Discussion
### Genomic Features  
The draft genome of WLY-B-L8T consisted of 93 contigs and 4808568 bp (N50, 89695; G+C content, 35.97 mol%; accession number, JAVBXD000000000). The genome sequence of WLY-B-L8T was 4.8 Mb in size. The G+C content of the type strain was determined as 35.97 mol%; in comparison, those of _B. rhizoplane_ JJ-63T, _B. pseudomycoides_ DSM 12442T, and _B. cytotoxicis_ NVH 391-98T were 36.39, 35.37, and 35.87 mol%, respectively [27]. Gene prediction and annotation led to 4781 coding sequences. The genome sequence-based digital DNA-DNA hybridization (dDDH) values between _Bacillus rhizoplane JJ-63T, Bacillus pseudomycoides_ DSM 12442T, and _Bacillus cytotoxicus_ NVH 391-98T were 36.70%, 26.10, and 23.90%, respectively. These values are below the threshold value for the 70% dDDH species boundary [30], demonstrating that WLY-B-L8T does not represent any of these related species. In addition, the average nucleotide identity (ANI) values between strain WLY-B-L8T and the three aforementioned type strains were 88.24%, 80.57, and 78.70%. The average amino identity (AAI) values between them are 89.84%, 79.51%, and 80.41%. All of the ANI and AAI values are below the 95-96% cut-off values [31], indicating that WLY-B-L8T represents a novel species of the genus _Bacillus_. The evolutionary relationships among bacteria can be studied by using orthologous genes of the core genome. The inference of the corresponding phylogenetic tree offers deeper insights. Hence, a phylogenomic analysis was conducted based on the 1041 core \begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Fatty Acid** & **1** & **2** & **3** & **4** \\ \hline C\({}_{12.0}\) & - & ND & 1.4 & ND \\ C\({}_{14.0}\) & 1.1 & 3.2 & 3.2 & 3.2 \\ C\({}_{16.0}\) & 3.9 & 8.6 & 9.0 & 5.6 \\ C\({}_{15.1}\)u5\(c\) & 1.9 & ND & ND & ND \\ C\({}_{16.1}\)u6\(c\) & - & 8.4 & 12.3 & ND \\ C\({}_{16.0}\)N Alcohol & 1.2 & ND & ND & ND \\ C\({}_{17.0}\) & 1.3 & ND & ND & ND \\ C\({}_{18.0}\) & 1.7 & ND & TR & ND \\ C\({}_{18.3}\)u6\(c\) & 4.3 & ND & ND & ND \\ _iso_-C\({}_{12.0}\) & 4.8 & ND & 8.7 & ND \\ _iso_-C\({}_{13.0}\) & 7.9 & 8.1 & 12.6 & 7.1 \\ iso-C\({}_{14.0}\) & 1.3 & 2.9 & 5.5 & 2.3 \\ iso-C\({}_{15.0}\) & 23.0 & 19.9 & 13.3 & 38.6 \\ iso-C\({}_{16.0}\) & 3.5 & 6.9 & 8.3 & 5.1 \\ iso-C\({}_{16.1}\)cis10 & - & 2.9 & - & - \\ iso-C\({}_{16.1}\)u5 & - & ND & 2.9 & ND \\ iso-C\({}_{17.0}\) & 8.9 & 16.9 & 7.0 & 11.4 \\ iso-C\({}_{17.1}\)u5\(c\) & 7.0 & ND & ND & ND \\ iso-C\({}_{17.1}\)u6 & - & ND & 2.3 & ND \\ _antieiso_-C\({}_{13.0}\) & 6.3 & 3.5 & 4.9 & ND \\ _antieiso_-C\({}_{15.0}\) & 2.9 & 10.6 & 3.6 & 3.1 \\ _antieiso_-C\({}_{17.0}\) & 1.4 & 3.3 & 1.6 & ND \\ _antieiso_-C\({}_{17.1}\)_a_ & 2.1 & ND & ND & ND \\ _antieiso_-C\({}_{17.1}\)_cis_5 & - & 4.6 & - & - \\ _antieiso_-C\({}_{17.1}\)u06 & - & ND & 1.1 & ND \\ Summed feature 2 & 2.6 & - & ND & 1.5 \\ Summed feature 3 & 6.7 & - & ND & 11.5 \\ \hline \hline \end{tabular} Values are percentages of the total fatty acids. Only fatty acids showing relative amounts \(>1\%\) are given. Strains 1, WLY-B-L8T; 2, _Bacillus rhizoplane JJ-63T, 3, _Bacillus pseudomycoides_ DSM 12442T, and 4, _Bacillus cytotoxicis_ NVH 391–98T. Data for strains 2–4 from [27]. ND, no data; TR, trace.  
\end{table} 
Table 2: Comparative FAME profiles of strain WLY-B-L8T and closely related phylogenetic neighbors in the genus _Bacillus_. marker genes. The phylogenetic tree (Figure 3) also demonstrated a clear clustering of WLY-B-L8T with the _Bacillus_ sp. The strain WLY-B-L8T was predicted and contained 4781 coding sequences. Gene function analysis using KEGG showed that the majority of the genes were involved in global and overview maps (693 genes), amino acid metabolism (266 genes), carbohydrate metabolism (222 genes), the metabolism of cofactors and vitamins (162 genes), energy metabolism (143 genes), nucleotide metabolism (86 genes), and lipid metabolism (76 genes). Among the COG-recognized genes, 256 genes were related to an unknown function.