# Assessment of Postbiotic, Mundticin-like Substance EM 41/3 Application in Broiler Rabbits
## 3 Results  
### Microbiota Evaluation  
The total count of enterococci in rabbit feces reached 5.81 \(\pm\) 0.22 CFU/g on average (log 10) at day 21 (3 weeks of MLS EM 41/3 application (Table 1)). At that time, their counts were significantly higher in EG rabbits compared to day 0/1 (ab \(p<0.001\)). But the enterococcal amount was also significantly higher in EG rabbits compared to CG rabbits (bc \(p<0.001\)). At day 42, higher counts of the total enterococci were found in EG rabbits compared in CG rabbits (difference 1.19 cycle) and also compared to day 0/1 (difference 1.35 cycle). The total counts of LAB, staphylococci, amyloidytic streptococci and coliforms in feces of broiler rabbits were not reduced by MLS EM 41/3 (Table 1).  
The count of total enterococci in caecum was lower than in feces. At days 21 and 42, enterococci reached up to 1.43 CFU/g (log 10) in caecum (Table 2). At day 21, LAB count decreased in caecum of the EG compared of the CG (difference 0.67 cycle). Staphylococcus were found almost in the same amount in caecum and feces of the EG at day 21 (Table 2) and they were slightly reduced in caecum at day 21 (difference 0.11 cycle). At day 42, even a significant decrease in staphylococci was found in rabbits of the EG (ab \(p<0.05\)) compared to rabbits in the CG. Amyloidytic streptococci in caecum were almost 1.0 log cycle lower than in feces (Table 2). At days 21 and 42, the count of amyloidytic streptococci was slightly higher in rabbits of the EG than in the CG (difference 0.19, respectively 0.22 cycle).  
In appendix, enterococci reached up to 2.54 CFU/g (log 10) at day 21 (Table 2). At day 42, enterococci reached up to 1.0 CFU/g (0.90) in appendix. Their counts were higher in rabbits of the EG than in rabbits of the CG. At day 21, amount of LAB was higher in rabbits of the EG than in rabbits of the CG. However, at day 42, LAB count decreased up to almost 1.0 CFU/g. Coliform bacteria and amyloidytic streptococci reached almost similar amounts in appendix as counted in caecum at days 21 and 42. Coliform bacteria and amyloidytic streptococci were not reduced in appendix.  
A total of 50% of re-isolated bacterial colonies from feces, caecum and appendix were susceptible to MLS EM 41/3.  
\begin{table}
\begin{tabular}{c c c c c c} \hline  & **Day 0/1 (_n_ = 10)** & **Day 21 (_n_ = 5)—EG** & **Day 21 (_n_ = 5)—CG** & **Day 42 (_n_ = 5)—EG** & **Day 42 (_n_ = 5)—CG** \\ \hline Enterococci & 3.39 \(\pm\) 1.45 \({}^{\rm a}\) & 5.81 \(\pm\) 0.22 \({}^{\rm b}\) & 4.40 \(\pm\) 0.79 \({}^{\rm c}\) & 4.74 \(\pm\) 0.47 & 3.39 \(\pm\) 0.48 \\ \hline Lactic acid bacteria & 3.79 \(\pm\) 1.23 & 5.67 \(\pm\) 0.22 & 4.94 \(\pm\) 0.75 & 4.44 \(\pm\) 0.82 & 3.25 \(\pm\) 0.40 \\ \hline Staphylococcus cocci & 3.98 \(\pm\) 0.50 & 4.28 \(\pm\) 0.34 & 3.91 \(\pm\) 0.33 & 4.25 \(\pm\) 0.28 & 4.43 \(\pm\) 0.57 \\ \hline Amyl. streptococci & 5.71 \(\pm\) 0.21 & 5.76 \(\pm\) 0481 & 5.24 \(\pm\) 0.61 & 5.99 \(\pm\) 0.37 & 5.89 \(\pm\) 0.24 \\ \hline Coliform bacteria & 2.28 \(\pm\) 1.72 & 5.37 \(\pm\) 0.73 & 4.83 \(\pm\) 1.16 & 4.18 \(\pm\) 1.27 & 3.85 \(\pm\) 0.64 \\ \hline \end{tabular}
\end{table}
Table 1: Bacterial counts in feces of rabbits after MLS EM 41/3 application (21 day) and its cessation (day 42) expressed in colony forming units per g (CFU/g, log 10).