## 3 Results
### Antibiofilm Activity  
Microorganisms have the ability to grow while irreversibly attached to a surface, forming complex communities known as biofilms. Currently, there is a search for the use of natural products as a source of antimicrobial compounds coupled with antib biofilm activity. Mushrooms are a promising source of bioactive compounds encompassing these bioactivities [51]. Regarding the effect of the present mushroom extracts on the inhibition of biofilm formation by different bacteria, the results are shown in Figure 4. For each microorganism, three concentrations of each extract were tested, namely the corresponding MIC, one-half of the MIC, and one-fourth of the MIC. In cases where no MIC was observed, the concentrations tested were 40, 20, and 10 mg/mL. Due to an insufficient amount of extract, it was not possible to test the antib Biofilm activity of the _P. ergngi_ M2A extract. The results obtained in Figure 4 reveal that most of the extracts were able to inhibit biofilm formation from the different bacteria, with a high number of extracts being able to inhibit up to 100% of biofilm formation. Even in the cases where no MIC was observed for the antibacterial activity, a high number of extracts was able to inhibit biofilm formation of the bacteria in concentrations ranging from 40 to 10 mg/mL. All extracts, with some exceptions, were able to cause significant biofilm inhibition even at concentrations of one-fourth of the MIC. In some cases, it was observed that inhibition at one-half of the MIC or one-fourth of the MIC was higher than at the MIC or 40 mg/mL. This can be attributed to the extracts not being completely solubilized in the medium at higher concentrations. At lower concentrations, solubilization improved, allowing for the inhibitory compounds to be more homogenously distributed in the wells. The results also show that antib biofilm activity was species-dependent, with extracts being more efficient at inhibiting certain bacteria and not others. For instance, the _P. ostreatus_ M2B inhibited 87.2-99.3% of _L. monocytogenes_, _P. fluorescens_, _P. tolaasii_, and _S. aureus_ biofilms but only 19.7-24.9% of _E. coli_ and _P. aeruginosa_ biofilms. Despite being the highest biofilm mass producer, _S. aureus_ was the most susceptible bacteria to the extracts, with all extracts causing inhibition ranging from 93.2 to 100%. Overall, the mushroom that showed better antib Biofilm activity was _A. cylindracea_, with more extracts being able to inhibit up to 100% of biofilm formation, followed by _P. ostreatus_. _P. ergngi_ showed the weakest antib biofilm activity among the three mushrooms. Of the three _A. cylindracea_ extracts, extract M2A showed the highest antib Biofilm capacity. This extract demonstrated antibacterial activity against five of the tested bacteria, strong antioxidant activity, and a high total phenolic content, which may explain its ability to inhibit biofilm formation.  
In [PERSON] et al. [51], mushroom extracts presented high inhibition percentages toward biofilm productions of different Gram-positive and Gram-negative bacteria. In another study by [PERSON] et al. [52], an _Agrocybe aegita_ methanolic extract was able to reduce _P. aeruginosa_ biofilm formation by 84.24% with a concentration of one-half of the MIC. Despite these reports, there are still few studies evaluating the antib Biofilm activity of mushroom extracts, especially aqueous extracts, which is why it is important to investigate the mechanism of action by which they inhibit biofilm production. The results obtained in the present study reveal the high capacity of mushroom extracts to inhibit bacterial biofilm formation.