### NIRS Prediction Equations (Calibration and External Validation)
#### 3.3.1 Lipid Content
\begin{table}
\begin{tabular}{c c c c c c c c c c c c c c c} \hline \hline \multirow{2}{*}{**Parameter**} & \multirow{2}{*}{**Test**} & \multirow{2}{*}{**Math Treatment**} & \multirow{2}{*}{**Range (nm)**} & \multirow{2}{*}{**LVs**} & \multicolumn{4}{c}{**Calibration**} & \multicolumn{4}{c}{**Cross Validation**} & \multicolumn{4}{c}{**External Validation**} \\ \cline{6-13}  & & & & \(\mathbf{R_{2}^{*}}\) & **RMSEC** & **1-VR** & **RMSEC** & **RPD\({}_{\mathbf{ov}}\)** & **RER\({}_{\mathbf{ov}}\)** & **R2\({}_{\mathbf{v}}\)** & **RMSEV** & **RPD\({}_{\mathbf{v}}\)** & **RER\({}_{\mathbf{v}}\)** \\ \hline IMF (g/100 g) & 1 & SNV+DT & 1000-2500 & 2 & 0.60 & 1.47 & 0.56 & 1.55 & 1.50 & 7.72 & 0.23 & 1.79 & 1.14 & 5.08 \\  & & & & & fatty acid composition (g/100 g FAMEs) & & & & & & & & & \\ Cl60 & 1 & SNV+DT + 1,11,15 & 1000-2500 & 7 & 0.60 & 1.05 & 0.35 & 1.34 & 0.95 & 0.82 & 0.26 & 1.28 & 1.17 & 4.91 \\ Cl80 & 1 & - & 1000-2500 & & \textless{}0.20 & - & \textless{}0.20 & - & - & - & \textless{}0.20 & - & - & - \\ Cl81:n-9 & 3 & MSC+2,10,9 & 1000-2500 & 4 & 0.63 & 1.32 & 0.41 & 1.78 & 1.25 & 5.09 & 0.17 & 2.07 & 1.15 & 5.52 \\ Cl82:n+6 & 1 & SNV+DT & 1000-2500 & 5 & 0.43 & 1.26 & 0.30 & 1.41 & 1.69 & 5.80 & 0.44 & 1.32 & 1.33 & 5.72 \\ Cl83:n-3 & 1 & SNV+DT & 1000-2500 & 5 & 0.34 & 0.10 & 0.20 & 0.14 & 1.12 & 5.78 & \textless{}0.25 & 0.12 & 1.11 & 4.53 \\ SEA & 1 & & & 1000-2500 & 1 & \textless{}0.20 & - & \textless{}0.20 & - & - & - & - \\ MUFA & 1 & SNV+DT + 1,11,15 & 1000-2500 & 6 & 0.43 & 2.08 & 0.29 & 2.61 & 1.05 & 4.92 & 0.55 & 1.91 & 1.49 & 6.89 \\ PUFA & 1 & SNV+DT + 1,11,15 & 1000-2500 & 5 & 0.50 & 1.68 & 0.36 & 1.94 & 1.23 & 5.41 & 0.54 & 1.71 & 1.47 & 6.47 \\ PUFA n+6 & 1 & SNV+DT + 1,11,15 & 1000-2500 & 4 & 0.56 & 1.49 & 0.33 & 1.80 & 1.20 & 5.40 & 0.55 & 1.55 & 1.49 & 6.24 \\ PUFA n+3 & 1 & SNV+DT & 1000-2500 & 10 & 0.47 & 0.16 & 0.41 & 0.17 & 1.28 & 5.00 & 0.22 & 0.19 & 1.16 & 4.91 \\ \hline \hline \end{tabular} Test 1: Calibration test composed of 98% of samples and 20% external validation test: Test 3: calibration test composed of 40% of samples and 60 and external validation test: Standard normal variate (SNV); de-trending (DT); multiplicative scatter correction (MSC). Latent variables (LVs) or number of partial least square (PLS) terms; coefficient of determination in calibration (R\({}_{\mathrm{C}}\); red non mean square error of calibration (RMSEC); red non mean square error of calibration (RMSEC); red non mean square error of calibration (RMSEC); red prediction deviation in cross validation (RRD); \(\epsilon\) range error ratio in cross validation (RER\({}_{\mathrm{C}}\)); coefficient of determination of external validation (R\({}_{\mathrm{C}}^{\prime}\)); red non mean square error of external validation (RMSEV); residual validation (RMSEV); residual prediction deviation in external validation (RRD); range error ratio in external validation (RER\({}_{\mathrm{C}}\)); red non mean square error of external fatty acids (SAF) detected (C120 + C130 + C140 + C150 + C160 + C170 + C180 + C200 + C220). Sun of all monomusterated fatty acids (MFA) detected (C141 + S + C161 + n + C161 n + S + C181 n + P + C201 n + S + C21 n + S + C21 n + S + C21 n + S + C221 n + S + C164 n + N + D100 + C20 + C20 + C20 + C20 + n + 6 + C203 n + C20 + S + C20 + S + C22 + n + 6 + C225 n + 3).  
\end{table} 
Table 4: Near-infrared spectroscopy (NIRS) predictive equations (calibration and external validation) for intramuscular fat (IMF) and fatty acid profile in intact _Longisisinus thoracis et lunchorum_ muscle.