# The Effect of Hydrolysis on the Antioxidant Activity of Olive Mill Waste
## 3 Results
### Antioxidant Activity
#### 3.2.2 Metal Ion Reducing Activity
\begin{table}
\begin{tabular}{c c c c c} \hline \hline  & & \multicolumn{2}{c}{_ABTS_} \\ \hline \multirow{2}{*}{**Cultivar**} & \multirow{2}{*}{**Date**} & \multicolumn{2}{c}{_IC\({}_{50}\) of Alkaline_} & \multicolumn{2}{c}{_IC\({}_{50}\) of Acid_} & \multicolumn{2}{c}{_IC\({}_{50}\) of_} \\  & & _Hydrolysis_ & _Hydrolysis_ & _Microeration_ \\ \hline _Bidni_ & 26 October 2021 & 84.68 \(\pm\) 8.80 & 191.10 \(\pm\) 6.18 & 1837.88 \(\pm\) 39.51 \\ _Bidni_ & 2 November 2021 & 43.91 \(\pm\) 20.95 & 90.77 \(\pm\) 42.81 & 12.81 \(\pm\) 12.61 \\ _Bidni_ & 16 November 2021 & 0.09 \(\pm\) 0.04 & 0.11 \(\pm\) 0.03 & 0.27 \(\pm\) 0.09 \\ BDS & 28 September 2021 & 45.62 \(\pm\) 3.55 & 53.88 \(\pm\) 4.08 & 9.80 \(\pm\) 13.89 \\ Carolea & 7 October 2021 & 109.75 \(\pm\) 17.59 & 6.11 \(\pm\) 7.86 & 839.02 \(\pm\) 152.85 \\ Carolea & 22 October 2021 & 98.94 \(\pm\) 57.31 & 3.10 \(\pm\) 2.96 & 800.71 \(\pm\) 173.90 \\ Carolea & 17 November 2021 & 0.46 \(\pm\) 0.14 & 0.65 \(\pm\) 0.15 & 1.44 \(\pm\) 0.13 \\ Cerasuola & 2 November 2021 & 0.05 \(\pm\) 0.01 & 86.20 \(\pm\) 5.53 & 615.52 \(\pm\) 45.12 \\ Coratina & 27 September 2021 & 30.32 \(\pm\) 22.23 & 4.05 \(\pm\) 2.37 & 800.30 \(\pm\) 150.70 \\ Coratina & 25 October 2021 & 72.72 \(\pm\) 0.97 & 95.02 \(\pm\) 6.21 & 295.80 \(\pm\) 50.56 \\ Frantooi & 27 September 2021 & 247.11 \(\pm\) 71.83 & 574.30 \(\pm\) 510.34 & 591.98 \(\pm\) 136.01 \\ Frantooi & 4 October 2021 & 217.80 \(\pm\) 53.50 & 199.43 \(\pm\) 219.36 & 45.27 \(\pm\) 29.93 \\ Frantooi & 19 October 2021 & 79.28 \(\pm\) 2.56 & 50.35 \(\pm\) 6.00 & 24.34 \(\pm\) 8.83 \\ Leccino & 17 October 2021 & 0.18 \(\pm\) 0.05 & 0.04 \(\pm\) 0.02 & 0.83 \(\pm\) 0.06 \\ _Malti_ & 29 September 2021 & 0.19 \(\pm\) 0.07 & 0.17 \(\pm\) 0.08 & 0.61 \(\pm\) 0.13 \\ Mixed 1 & 2 October 2021 & 0.77 \(\pm\) 0.46 & 44.39 \(\pm\) 4.19 & 135.78 \(\pm\) 40.30 \\ Mixed 2 & 30 September 2021 & 74.14 \(\pm\) 5.17 & 77.38 \(\pm\) 14.79 & 14.26 \(\pm\) 8.40 \\ _Bajda 1_ & 4 October 2021 & 3.13 \(\pm\) 3.64 & 185.65 \(\pm\) 6.43 & 30.54 \(\pm\) 14.41 \\ _Bajda 2_ & 4 October 2021 & 5.82 \(\pm\) 4.10 & 48.35 \(\pm\) 3.44 & 174.72 \(\pm\) 51.97 \\ Picholine & 7 October 2021 & 100.21 \(\pm\) 13.93 & 0.48 \(\pm\) 0.50 & 262.26 \(\pm\) 63.37 \\ UDP & 4 October 2021 & 0.39 \(\pm\) 0.67 & 0.63 \(\pm\) 1.09 & 106.88 \(\pm\) 58.25 \\ UDP & 7 October 2021 & 65.00 \(\pm\) 11.05 & 0.03 \(\pm\) 0.02 & 269.47 \(\pm\) 23.13 \\ UDP & 25 October 2021 & 0.56 \(\pm\) 0.67 & 0.27 \(\pm\) 0.24 & 157.46 \(\pm\) 62.23 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: IC\({}_{50}\)s of olive oil extracts for _ABTS_ radical cation stabilization assays. (BDS—Bella di Spagna and UDP—Uovo di Piccione). Figure 6: Mean of cupric reducing antioxidant activity of olive mill waste extracts using three different solvent extraction methods—methanol, acid and alkaline (black, red and green bars, respectively) with error bars showing \(\pm\) 1 SD for three independent trails. in Cerasuola (0.57 \(\pm\) 0.39). FRAP reducing activity was expressed in mg AAE/g (ascorbic acid equivalent).