## 3 Results  
### Influence of the Nature of the Substrates on the Oxidants and on Their Antifungal Activity  
After the LPO oxidoreductase of 1.2 mM KSCN + 5.4 mM KI + 6.6 mM H2O2, 5.4 mM KI + 5.4 mM H2O2, and 1.2 mM KSCN + 1.2 mM H2O2, as well as the removal of the enzyme, the three solutions were kept at 25 \({}^{\circ}\)C. The oxidation of thiol groups and the biological activity against _P. expansum_ was measured over 10 days. Results are presented in Table 2. No inhibition was observed with the oxidant solutions prepared from SCN\({}^{-}\) or I\({}^{-}\). The oxidant solutions were prepared with a mixture of SCN\({}^{-}\) and I\({}^{-}\) inhibited fungal growth by 80 to 94% for 10 days. Catalytic oxidation of iodide produced stable low levels of oxidant for up to 10 days, with a yellowing after enzymatic oxidation. Iodine was titrated with sodium thiosulfate at 0.092 mM. Catalytic oxidation of thiocyanate produced high levels of oxidant on the production day and then a sharp loss after 1 day of storage, with a half-life of less than 1 day. Ion chromatography revealed the presence of SCN\({}^{-}\), SO4\({}^{2-}\), OCN\({}^{-}\), and OSCN\({}^{-}\) (Figure 1). \begin{table}
\begin{tabular}{c c c c} \hline \hline  & \multicolumn{3}{c}{**Substrate Doses**} \\ \hline  & **1.2 mM KSCN +** & **1.2 mM KSCN + 5.4 mM KI** & **5.4 mM KI + 5.4 mM H2O2** \\  & **1.2 mM H2O2** & & **+ 6.6 mM H2O2** & \\ \hline
**Storage (days)** & \multicolumn{3}{c}{_P. expansum_ **inhibition (\%)**} \\ \hline
0 & 0 \(\pm\) 4 ns & 94 \(\pm\) 3 a & 0 \(\pm\) 1 ns \\
1 & - & 94 \(\pm\) 8 a & - \\
2 & - & 90 \(\pm\) 3 a & - \\
3 & - & 86 \(\pm\) 3 a & - \\
5 & - & 94 \(\pm\) 5 a & - \\
10 & - & 80 \(\pm\) 5 a & - \\ \hline  & \multicolumn{3}{c}{**Oxidant Concentration (\(\mu\)M)**} \\ \hline
0 & 359 \(\pm\) 24 & 405 \(\pm\) 80 & 54 \(\pm\) 6 \\
1 & 72 \(\pm\) 9 & 381 \(\pm\) 28 & 55 \(\pm\) 4 \\
2 & 17 \(\pm\) 7 & 361 \(\pm\) 34 & 52 \(\pm\) 4 \\
3 & 10 \(\pm\) 5 & 362 \(\pm\) 28 & 45 \(\pm\) 6 \\
5 & 0 \(\pm\) 0 & 277 \(\pm\) 50 & 49 \(\pm\) 11 \\
10 & 0 \(\pm\) 0 & 263 \(\pm\) 28 & 52 \(\pm\) 9 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: In vitro inhibition % of _P. expansum_ and \(-\)SH oxidant concentration (\(\mu\)M) with various doses of substrates after oxidoreductase by lactoperoxidase and storage at 25 \({}^{\circ}\)C for 0, 1, 2, 3, 5, or 10 days. [PERSON]’s comparison of means was applied with 95% confidence; means that do not share a letter are significantly different; ns, not significantly different from the control. Means and s.d. of two independent experiments. Catalytic oxidation of the iodide-thiocyanate mixture produced a colorless solution containing stable and highly dosed oxidant with a shelf-life of more than 10 days (Table 2). Iodine titration was performed; no iodine was measured. Ion chromatography of the SCN\({}^{-}\)- and I\({}^{-}\)-derived solution detected I\({}^{-}\), SCN\({}^{-}\), and SO\({}_{4}\)\({}^{2-}\), but did not detect any OSCN\({}^{-}\), OCN\({}^{-}\), or OI\({}^{-}\) (Figure 1). \({}^{13}\)C NMR was performed on two solutions: 1.2 mM KS\({}^{13}\)CN + 1.2 mM H\({}_{2}\)O\({}_{2}\) and 1.2 mM KS\({}^{13}\)CN + 5.4 mM KI + 6.6 mM H\({}_{2}\)O\({}_{2}\) (Figure 2). It confirmed the presence of OCN\({}^{-}\) and OSCN\({}^{-}\) in the SCN\({}^{-}\)-oxidized solution [26] and the absence of OSCN\({}^{-}\), OCN\({}^{-}\) or interpseudohalogen molecules, such as ICN, I(SCN)\({}_{2}\)\({}^{-}\), and I\({}_{2}\)SCN\({}^{-}\) in the SCN\({}^{-}\)- and I\({}^{-}\)-oxidized solutions [25]. Figure 1: Ion chromatography of (**A**) 1.2 mM KSCN + 1.2 mM H\({}_{2}\)O\({}_{2}\) and (**B**) of 1.2 mM KSCN + 5.4 mM KI + 6.6 mM H\({}_{2}\)O\({}_{2}\) after oxidoreduction by lactoperoxidase in Milli-Q\({}^{\bigotimes}\) water. Representative spectra of two independent experiments is given.