## 3 Results
### Chemical Composition of Enzymatically Treated LP  
Enzyme preparations used for carbohydrate hydrolysis were selected based on research by other scientists. [PERSON] et al. [22] found that treating berry pulp with Viscozyme(r) L and Pectinex(r) Ultra SP significantly increased the extraction yield and total soluble solids compared to juice pressing without enzymes. [PERSON] et al. [23] found that processing the pomace with Celluclast(r) 1.5 L resulted in the highest pectin yield among tested enzymatic preparations. The research findings suggest that this enzymatic preparation has a broad action profile capable of liberating pectins from the cell walls of plants rich in cellulose and hemicellulose. All used enzyme preparations reduced (\(p<0.05\)) the TDF content in LP and LP-CO\({}_{2}\) (Table 2) compared to the enzymatically untreated samples (Table 1). The highest reduction in TDF was reached using Viscozyme(r) L and Pectinex(r) Ultra Tropical (19.8 and 18.9% in LP and 18.9 and 17.3% in LP-CO\({}_{2}\), respectively). A smaller reduction in TDF was found using Celluclast(r) 1.5 L for LP and LP-CO\({}_{2}\) modifications (6.8 and 6.6%, respectively). SDF was hydrolyzed at a higher degree compared with IDF using Viscozyme(r) L and Pectinex(r) Ultra Tropical. The reduction in SDF reached 53.1 and 53.6% in LP, while it reached 54.6 and 49.29% in LP-CO\({}_{2}\), respectively. A high reduction in SDF had an adverse effect on the IDF/SDF ratio in the modified LP products. The results show that smaller fragments of hydrolysis can be obtained which cannot be precipitated with ethanol. Opposite trends were found when Celluclast(r) 1.5 L was used for LP enzymatic treatment. This enzyme preparation increased (\(p<0.05\)) the content of SDF in LP by 13% and in LP-CO\({}_{2}\) by 11.6% \begin{table}
\begin{tabular}{c c c} \hline \hline
**Parameter** & **LP** & **LP-CO\({}_{2}\)** \\ \hline Moisture, \% & 3.41 \(\pm\) 0.04  & 3.58 \(\pm\) 0.09  \\ Crude protein & 8.60 \(\pm\) 0.28  & 9.03 \(\pm\) 0.01  \\ Lipids & 12.68 \(\pm\) 0.39  & 7.60 \(\pm\) 0.04  \\ Ash & 1.18 \(\pm\) 0.01  & 1.24 \(\pm\) 0.01  \\ TDF & 73.85 \(\pm\) 0.79  & 77.20 \(\pm\) 1.62  \\ IDF & 65.36 \(\pm\) 0.69  & 68.26 \(\pm\) 1.47  \\ SDF & 8.49 \(\pm\) 0.1  & 8.94 \(\pm\) 0.15  \\ IDF/SDF ratio & 7.698 & 7.635 \\ \({}^{1}\) Mono- and oligosaccharidesaries = 100 – (crude protein + lipids + ash + IDF). Mean \(\pm\) standard deviation values in row with different lowercase letters are significantly different (\(p<0.05\)).[ENDFOOTNOTE] & 3.68 \(\pm\) 1.22  & 4.93 \(\pm\) 1.20  & 1.20 \(\pm\) \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Chemical composition of LP before and after SFE-CO\({}_{2}\) extraction (LP-CO\({}_{2}\)), g/100 g DM. compared to the untreated raw material, and the IDF/SDF ratio for LP was improved from 7.6 to 6.1, while that for LP-CO\({}_{2}\) improved from 7.6 to 6.2; however, this ratio did not reach the recommended value. The IDF content was reduced more effectively using Viscozyme(r) L and Pectinex(r) Ultra Tropical (15.5 and 14.4% in LP and 14.3 and 13.1% in LP-CO\({}_{2}\), respectively) compared with Celluclast(r) 1.5 L (9.4 and 9.0% in LP and LP-CO\({}_{2}\), respectively). The partial separation of lipophilic substances during SFE-CO\({}_{2}\) extraction did not have a statistically significant effect on the enzymatic hydrolysis efficiency. There is a lack of data in the literature on the modification of LP with enzymes or other pre-treatments with the aim to increase the amount of SDF, while cereal and legume by-products with possibilities of increasing the SDF content are widely studied through applying not only enzymatic treatments, but also physical treatments, such as high hydrostatic pressures and thermal and extrusion techniques [24]. Treatment with high hydrostatic pressures increases the SDF content in mango and orange peel by 15 and 95%, respectively, while applying extrusion treatment for garlic skin and wheat bran increased the SDF content by 199 and 98%, respectively [24]. [PERSON] et al.