## 3 Results
### Taxonomic Identification of the Oral Microbiome  
A total of seven major bacterial phyla (_Spirochaetes, Firmicutes, Fusobacteria, Actinobacteria, Proteobacteria, Bacterioides_, and _Sacharibacteria_TM_) were identified at a higher than 1% frequency in all the plaque samples (Figure 5A). The peri group showed an average of 5.7 phyla. Only sample 36-2 showed seven phyla, including _Spirochaetes_. Seven samples showed six phyla and six samples showed five phyla. The endo group showed an average of 3.5 phyla and sample 36-4 showed two phyla.  
Figure 5B shows that at the genus level, _Veillonella, Streptococcus_, and _Leptotrichia_ were present at 5% frequency in almost all the peri group samples. _Parvinonas, Peptostreptococcus, Atopobium_, and _Dialister_ were present in all endo group samples. Other genera were found in several samples in relation to the habitat or tooth. _Aggregatibacter actinomyces_ were not identified.  
Figure 5C shows the analysis of the endo group samples at a frequency of 1%. _Bulleidia_ was found in all four samples. _Fusobacterium, Mogibacterium, Prevotella_, and _Olsenella_ were present in three samples. _HQ41590\(g\) was found in two samples, while _Shuttleworthia, Alloprerotella, Eubacterium_g10, Selenomonas_, and _Oribacterium_ were present in one sample.  
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multirow{2}{*}{**Group**} & \multirow{2}{*}{**Sample ID**} & \multicolumn{3}{c}{**Taxonomic Identification**} & \multicolumn{3}{c}{**\(\alpha\)-Diversity**} \\ \cline{3-6}  & & \multicolumn{2}{c}{**Number of Final Reads**} & \multicolumn{2}{c}{**Number of OTUs *} & **[PERSON]** & **[PERSON] 1** & **Shannon** \\ \hline \multirow{8}{*}{**Period**} & Nor-1 & 18092 & 183 & 201.84 & 193.62 & 3.52 \\  & 11-1 & 19109 & 155 & 181.94 & 184 & 3.32 \\  & 16-1 & 18517 & 161 & 178.61 & 171.12 & 3.71 \\  & 26-1 & 18293 & 196 & 219.54 & 206.50 & 3.67 \\  & 36-1 & 18317 & 193 & 212.38 & 206.04 & 3.88 \\  & 46-1 & 19395 & 104 & 119.42 & 130.25 & 2.74 \\  & **Average** & **18621** & **165** & **185.62** & **181.92** & **3.47** \\ \cline{2-6}  & Nor-2 & 19134 & 206 & 250.28 & 243.27 & 3.70 \\  & 11-2 & 18492 & 262 & 319.99 & 304.78 & 3.84 \\  & 16-2 & 19032 & 185 & 217.45 & 216.95 & 3.54 \\  & 26-2 & 18528 & 201 & 213.69 & 207.56 & 4.01 \\  & 36-2 & 19629 & 206 & 238.47 & 248.50 & 3.58 \\  & 46-2 & 19491 & 133 & 176.67 & 163.00 & 2.78 \\  & **Average** & **19051** & **199** & **236.09** & **230.68** & **3.58** \\ \cline{2-6}  & 46-3 & 19952 & 185 & 215.64 & 224.18 & 3.24 \\ \hline \multirow{8}{*}{**Endo**} & 16-4 & 17033 & 52 & 67.61 & 61.43 & 2.01 \\  & 36-4 & 19238 & 59 & 90.32 & 94 & 1.98 \\ \cline{1-1}  & **Average** & **18136** & **56** & **78.97** & **77.72** & **2.00** \\ \cline{1-1} \cline{2-6}  & 16-5 & 16233 & 62 & 73.68 & 69.80 & 2.23 \\ \cline{1-1}  & 36-5 & 18570 & 68 & 150.03 & 98.00 & 2.17 \\ \cline{1-1}  & **Average** & **17402** & **65** & **111.86** & **83.9** & **2.2** \\ \hline \hline \end{tabular}  
* OTUs: operational taxonomical units.  
\end{table}
Table 2: Taxonomic identification and assessment of \(\alpha\)-diversity.  
Figure 5: **Relative abundance of oral bacteria at the phylum and genus levels.** (**A**) Relative abundance of major bacterial phyla with >1% frequency in all samples. (**B**) Relative abundance of major bacterial genera with >5% frequency in all samples. (**C**) Relative abundance of major bacterial genera with >1% frequency in the endodontic samples.