## 2 Results and Discussion
### Phytochemical Characterisation
#### 2.2.2 Physicochemical Characterisation by UHPLC
\begin{table}
\begin{tabular}{c c c c c c c c c c c c} \hline \hline  & & & & & & & & \multicolumn{4}{c}{**\% Area**} \\ \cline{5-12}
**No** & **RT** & _m/z_ & **Structural** & **Formula** & & & & _A.P var. & _pyrethrum_ & & & _A.P var. & _depressus_ & \\ \cline{5-12}  & & & & & & & & & & & & & \\ \hline
**1** & 0.60 & 180 & C\({}_{5}\)H\({}_{6}\)O\({}_{4}\) & Caffeic acid & – & – & – & 0.55 & – & – & – & – \\ \hline
**2** & 4.60 & 154 & C\(8\)H\({}_{10}\)O\({}_{3}\) & Hydroxytyrosol & 1.11 & 5.56 & 2.62 & 3.02 & 1.37 & 3.73 & 1.89 & 3.87 \\ \hline
**3** & 5.95 & 174 & C\({}_{5}\)H\({}_{4}\)N\({}_{4}\)O\({}_{2}\) & L-arginine & 15.76 & 16.12 & 2.29 & 3.20 & 15.02 & 1.68 & 1.49 & 2.82 \\ \hline
**4** & 6.64 & 170 & C\({}_{7}\)H\({}_{4}\)O\({}_{5}\) & Gallic acid & 6.52 & 6.09 & 8.95 & 11.00 & – & 4.54 & 7.16 & 11.91 \\ \hline
**5** & 7.26 & 223 & C\({}_{41}\)H\({}_{25}\)NO & Pelllorine & 0.18 & 1.07 & 0.85 & 1.25 & 0.15 & 0.35 & 0.57 & 0.46 \\ \hline
**6** & 12.77 & 290 & C\({}_{15}\)H\({}_{4}\)O\({}_{6}\) & Catechin & – & – & – & 7.23 & – & – & – \\ \hline
**7** & 16.98 & 168 & C\({}_{5}\)H\({}_{5}\)O\({}_{4}\) & Vanillic acid & 3.07 & – & 1.10 & 4.60 & 0.45 & – & 1.11 & 2.28 \\ \hline
**8** & 17.61 & 354 & C\({}_{6}\)H\({}_{5}\)O\({}_{3}\) & Chlorogenic acid & – & – & – & 1.05 & – & – & – \\ \hline
**9** & 21.69 & 146 & C\({}_{5}\)H\({}_{6}\)O\({}_{2}\) & Coumarin & 2.40 & 12.56 & 19.29 & 12.98 & 3.37 & 11.41 & 20.53 & 13.73 \\ \hline
**10** & 21.79 & 148 & C\({}_{5}\)H\({}_{6}\)O\({}_{2}\) & Cinnamic acid & 1.07 & – & – & 2.42 & – & – & – \\ \hline
**11** & 21.86 & 164 & C\({}_{5}\)H\({}_{5}\)O\({}_{3}\) & P-cumaric acid & – & – & – & 0.88 & – & – & – \\ \hline
**12** & 22.03 & 194 & C\({}_{6}\)H\({}_{4}\)O\({}_{4}\) & Transferulic acid & – & – & – & – & 1.00 & – & – \\ \hline
**13** & 22.15 & 194 & C\({}_{10}\)H\({}_{4}\)O\({}_{4}\) & Ferulic acid & – & – & – & – & – & 1.52 & – \\ \hline
**14** & 23.21 & 540 & C\({}_{25}\)H\({}_{23}\)O\({}_{3}\) & Oleluropein & – & 0.41 & 3.07 & 0.84 & – & 0.32 & 1.65 & 0.44 \\ \hline
**15** & 24.63 & 580 & C\({}_{27}\)H\({}_{32}\)O\({}_{4}\) & Naringin & – & 0.58 & 0.46 & 0.44 & – & 0.31 & 0.26 & – \\ \hline
**16** & 24.86 & 302 & C\({}_{15}\)H\({}_{40}\)O\({}_{7}\) & Quercetin & – & – & – & – & – & 0.18 & – \\ \hline
**17** & 25.96 & 154 & C\({}_{10}\)H\({}_{18}\)O\({}_{7}\) & Geraniol & – & – & 3.67 & 1.49 & – & – & – \\ \hline
**18** & 28.59 & 302 & C\({}_{16}\)H\({}_{4}\)O\({}_{6}\) & Hesperetin & – & – & – & – & 6.79 & 2.83 & 8.36 & \\ \hline
**19** & 38.72 & 237 & C\({}_{15}\)H\({}_{2}\)NO & Deca-2_AE_-dienoic acid & – & 0.39 & – & – & – & – & – & – \\ \hline
**20** & 42.03 & 271 & C\({}_{18}\)H\({}_{25}\)NO & Anacyclin & 0.67 & – & – & – & 0.44 & – & – & – \\ \hline \multirow{2}{*}{**21**} & \multirow{2}{*}{54.25} & \multirow{2}{*}{318} & \multirow{2}{*}{C\({}_{15}\)H\({}_{10}\)O\({}_{8}\)} & \multirow{2}{*}{((2_E_AE_)-N-(2-methylpropyl)tetradeca-2,4-diene-8,10-diynamide)} & \multirow{2}{*}{–} & \multirow{2}{*}{–} & \multirow{2}{*}{–} & \multirow{2}{*}{–} & \multirow{2}{*}{–} & \multirow{2}{*}{–} & \multirow{2}{*}{–} & \multirow{2}{*}{–} \\  & & & & & & & & & & & \\ \cline{5-12}  & & & & & & & & & & & \\ \hline \hline \end{tabular}
\end{table} 
Table 6: Chemical composition obtained by UHPLC of the different parts (roots, seeds, leaves, and capitula) of the two varieties _A.P_ var. _pyrethrum_ and _A.P_ var. _depressus_. our analysis and previous analyses could depend on several factors: the location and season of plant collection may result in a change in the active components, and the type of extraction solvent may change the active compounds extracted from the plant samples [71].