[[https://doi.org/10.3390/app112110416](https://doi.org/10.3390/app112110416)]([https://doi.org/10.3390/app112110416](https://doi.org/10.3390/app112110416))  
Academic Editor: [PERSON]  
Received: 22 September 2021  
Accepted: 28 October 2021  
Published: 5 November 2021  
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**Copyright:** 2021 by the authors. License MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([[https://creativecommons.org/licenses/by/4.0/](https://creativecommons.org/licenses/by/4.0/)]([https://creativecommons.org/licenses/by/4.0/](https://creativecommons.org/licenses/by/4.0/))). **Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China; [EMAIL_ADDRESS] (B.C. ); [EMAIL_ADDRESS] (H.Z. ); [EMAIL_ADDRESS] (D.D. ); [EMAIL_ADDRESS] (H.M.)  
\({}^{2}\) Department of Biomedical Engineering, Tsinghua University, Beijing 100084, China  
Department of Gastroenterology, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, China; [PERSON]IL_ADDRESS]  
\({}^{4}\) Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China  
\({}^{5}\) Tsinghua-Berkeley Shenzhen Institute, Tsinghua University, Shenzhen 518071, China  
\({}^{6}\) Department of Physics, Tsinghua University, Beijing 100084, China  
Correspondence: [EMAIL_ADDRESS] (H.H. ); [EMAIL_ADDRESS] (H.M.)  
These authors contributed equally to this work. **Abstract:** Mueller matrix polarimetry is widely used in biomedical studies and applications, for it can provide abundant microstructural information about tissues. Recently, several methods have been proposed to decompose the Mueller matrix into groups of parameters related to specific optical properties which can be used to reveal the microstructural information of tissue samples more clearly and quantitatively. In this study, we thoroughly compare the differences among the parameters derived from the Mueller matrix polar decomposition (MMPD) and Mueller matrix transformation (MMT), which are two popular methods in tissue polarimetry studies and applications, while applying them on different tissue samples for both backscattering and transmission imaging. Based on the Mueller matrix data obtained using the setups, we carry out a comparative analysis of the parameters derived from both methods representing the same polarization properties, namely depolarization, linear retardance, fast axis orientation and diattenuation. IN particular, we propose several modified MMT parameters, whose abilities are also analyzed for revealing the information about the specific type of tissue samples. The results presented in this study evaluate the applicability of the original and modified MMT parameters, then give the suggestions for appropriate parameter selection in tissue polarimetry, which can be helpful for future biomedical and clinical applications. **Keywords:** Mueller matrix polarimetry; optical properties; biomedical imaging