## 4 Discussion
The C1 molecule can be evaluated as an alternative antibacterial agent. C2 was found to be effective against _S. aureus_, _E. faecalis_ and _K. pneumoniae_. C2 was observed to be more effective than sulfararazine against _S. aureus_. [PERSON] et al. investigated the antimicrobial effect of C2 against _E. coli_, _S. aureus_, _B. cereus_, _P. aeruginosa_, and _B. subtilis_ by diffusion on agar method. They stated that C2 is effective against _S. aureus_, _B. cereus_, and _B. subtilis_[33]. The antibacterial effect of C2 against _S. aureus_ indicated in that study is consistent with our study. Although C2 did not show any activity against some microorganisms, it showed a potentially significant effect against some of them. The C2 molecule can also be considered an alternative to antibacterial agents. Tyrosinase enzyme inhibitors are important as depigmentation agents in the cosmetic and pharmaceutical industries, as well as as antibrowning compounds in the food and agricultural industries [34]. For these reasons, there is a need for new natural tyrosinase enzyme inhibitors. In this study, the TYR enzyme-inhibitory effect of C1 and C2 was tested. Kojic acid was used as positive control. Both C1 (24%) and C2 (32%) showed weak TRY-inhibitory effects. [PERSON] et al. investigated the tyrosinase enzyme-inhibitory activity of many compounds isolated from _Marrubium velutinum_ and _Marrubium cylindemeum_. The low effect of C2 (27.35 \(\pm\) 1.24) and apigenin glycosides in the study is consistent with our study [35]. [PERSON] et al. investigated the tyrosinase enzyme-inhibitory effect of some compounds isolated from _Crisium japonicum_ var. _maackii_ (Maxim.) Matsum. In the study, C2 showed weak activity (IC\({}_{50}\) = 177.03 \(\pm\) 0.77 \(\upmu\)M for l-tyrosine and 399.08 \(\pm\) 3.89 \(\upmu\)M for l-DOPA), while luteolin 5-O-\(\upbeta\)-D-glucopyranoside showed high activity (IC\({}_{50}\) values of 2.95 \(\pm\) 0.11 \(\upmu\)M for L-tyrosine and 8.22 \(\pm\) 0.18 \(\upmu\)M for L-DOPA). They stated that a glucose in the 5-OH position increased the activity compared to the 7-OH position [36]. In compounds C1 and C2, the sugar is attached to the seventh carbon. TYR-inhibitory effects were low in both molecules. This is compatible with the literature. AD is a progressive and widespread neurodegenerative disease associated with aging, and it is an urgent public health problem in many parts of the world [24]. There are many studies in the literature investigating the AChE-inhibitory effects of natural plants [22]. New drug molecule studies are extremely important for this incurable disease. AChE and BuChE enzymes are involved in this disease. In our study, AChE and BuChE enzyme-inhibition activities of C1 and C2 were investigated. According to the results of the study, C1 and C2 showed 30% and 65% AChE inhibition, respectively. Moreover, C1 and C2 showed 13% and 36% BuChE inhibition, respectively. The AChE-inhibitory activity of C2 was the highest. Therefore, the molecular interaction between C2 and AChE enzyme was evaluated in the next step. [PERSON] et al. evaluated the AChE-inhibition effects of extracts of _Dryariae_ rhizome and isolated compounds by a bioguided fractionation procedure. Consistently with our results, they reported that C2 showed an AChE-inhibitory effect (17.13 \(\pm\) 1.02 \(\upmu\)M) [37]. In addition, [PERSON] et al., investigated the AChE- and BuChE-inhibition effects of _Globularia meridionalis_ (Podp.) O. Schwarz and some isolated compounds. They reported that C1 exhibited inhibition against AChE with and IC\({}_{50}\) value of 172.6 \(\pm\) 1.4 \(\upmu\)g/mL and against BChE with an IC\({}_{50}\) value of 221.4 \(\pm\) 2.5 \(\upmu\)g/mL [38]. In our study, the C2 molecule showed a higher effect than the C1 molecule. This is compatible with the literature. It may be appropriate to conduct more detailed studies on the treatment of AD with the C2 molecule.