# _Applied_
###### Abstract  
The overproduction of reactive oxygen species (ROS) can cause oxidative stress to biomolecules such as nucleic acids, proteins, and lipids, leading to neurodegenerative disorders. _Sasa borealis_ (SB) has antioxidant, anti-inflammatory, antidiabetic, and anti-obesity effects. We evaluated the neuroprotective activity of SB on hydrogen peroxide (H\({}_{2}\)O\({}_{2}\))-induced oxidative stress. We investigated the antioxidant and neuroprotective effects of SB water extract (SBW) and SB ethanol extract (SBE) by measuring the radical scavenging activities and intracellular ROS production. SBE, which had a high level of isorientin, had higher antioxidative activities than SBW in 2,2'-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt (ABTS\({}^{+}\)) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. It also reduced ROS generation in pheochromocytoma 12 (PC12) cells more significantly than SBW. It increased the translation of heme oxygenase-1 (HO-1), superoxide dismutase 2 (SOD2), catalase (CAT), and glutathione peroxidase (GPx) with a corresponding increase in the translation of NF-E2-related factor-2 (Nrf-2). In conclusion, SBE with high levels of phenolic compounds such as isoorientin shows promise for preventing neurodegenerative diseases. Sasa borealisEthanol Extract Protects PC12 20221264553390  
wild grass distributed widely in Asian countries including South Korea, China, and Japan. Bamboo has been used in traditional medicine for its anti-inflammatory, antipyretic, and diuretic effects for centuries in South Korea and other Asian countries [6]. _S. borealis_ (SB) has antidiabetic [7], hypoglycemic, hypolipidemic [8], anti-obesity [9], and antioxidant [10] effects. In addition, the use of bamboo for the treatment of hypertension, arteriosclerosis, cardiovascular disease, and cancer has been reported [11]. The diverse biological and pharmacological properties of bamboo leaf extract are attributed to the presence of flavones, glycosides, phenolic acids, coumarone lactones, anthraquinones, and amino acids [12]. _S. borealis_ leaf extract has higher total phenolic and flavonoid contents compared to other bamboo species; the major phenolic compounds are protocatechuic acid, \(p\)-hydroxy benzoic acid, caffeic acid, syringic acid, \(p\)-coumaric acid, leuteolin-6-glucose, and tricine-7-glucose [13]. In addition, _S. borealis_ contains friedelin and glutiol (triterpenoids), isorientin, tricin 7-O-\(\beta\)-D-glucopyranoside (flavonoids), and luteolin 6-C-\(\alpha\)-L-arabinopyranoside [14]. Isoorientin (3\({}^{\prime}\),4\({}^{\prime}\),5,7-tetrahydro-6-C-glucopyranosyl flavone) and a common C-glycosyl flavone, luteolin-6-C glucoside, have been isolated from several plant taxa, such as _Patrinia villosa Juss_, _Crataegus monogyna_, _Crataegus pentagyna_, _Gentiana_, _Rumex_, _Swertia_, and _Vitex_[15; 16; 17]. Isoorientin has antioxidant, anti-inflammatory, antitumor, and hepatocyte protective effects [18; 19; 20]. Isoorientin is a flavonoid-like compound that exhibits various pharmacological activities such as antioxidant and anti-inflammatory properties. In [PERSON]'s study [21], isorientin reduced ROS production in A\(\beta\)25-35-induced BV2 cells, thereby blocking apoptosis and reportedly exhibiting anti-inflammatory action. According to [PERSON] [22] et al., flavonoid compounds protect cells from damage caused by oxidative stress, and isorientin exhibits neuropathic anti- nociceptive activity. Previous studies have demonstrated that isorientin has a cytoprotective effect by inducing Nrf2, increasing detoxification molecules such as HO-1 and NQO-1, and activating the Sirt1/Sirt6 pathway. Nuclear transcription factor-E2-related factor 2-antioxidant responsive element (Nrf2-ARE) is one of the important antioxidant pathways in neuronal cells.