## 3 Results
### Microbiome Analysis  
#### 3.5.1 Total Viable Counts (TVC)  
As is shown in Figure 1B, the TVC of the samples rapidly increased from the initial value of about 3.4 lgCFU/g to 8.15 lgCFU/g by the end of storage. After storage for 5 days, the TVC increased to 5.83 lgCFU/g, increasing by 71.47% compared with day 1. However, with the limit of 7.0 lgCFU/g for freshwater fish [30], the TVC of samples reached the limit (7.0 lgCFU/g) from 7 days to 9 days. After 11 days, the growth rate of microorganisms entered a stable period, an increase of 4.75 lgCFU/g compared with day 1. Therefore, the  
\begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline \multirow{2}{*}{**BAs (mg/kg)**} & \multicolumn{6}{c}{**Storage**} \\  & **0** & **1** & **3** & **5** & **7** & **9** & **11** & **13** \\ \hline TRP & 0.08 \(\pm\) 0.00 \({}^{\circ}\) & 0.29 \(\pm\) 0.04 \({}^{\circ}\) & ND & ND & ND & ND & ND \\ PEA & 0.04 \(\pm\) 0.00 \({}^{\circ}\) & 0.09 \(\pm\) 0.01 \({}^{\circ}\) & 0.14 \(\pm\) 0.02 \({}^{\circ}\) & 0.15 \(\pm\) 0.00 \({}^{\circ}\) & 0.24 \(\pm\) 0.02 \({}^{\circ}\) & 0.61 \(\pm\) 0.01 \({}^{\circ}\) & 1.93 \(\pm\) 0.08 \({}^{\circ}\) & 3.35 \(\pm\) 0.04 \({}^{\circ}\) \\ PUT & 0.04 \(\pm\) 0.01 \({}^{\circ}\) & 1.58 \(\pm\) 0.04 \({}^{\circ}\) & 2.46 \(\pm\) 0.13 \({}^{\circ}\) & 7.95 \(\pm\) 0.19 \({}^{\circ}\) & 12.57 \(\pm\) 0.38 \({}^{\circ}\) & 22.10 \(\pm\) 0.26 \({}^{\circ}\) & 41.41 \(\pm\) 0.20 \({}^{\circ}\) & 73.23 \(\pm\) 0.36 \({}^{\circ}\) \\ CAD & 0.57 \(\pm\) 0.19 \({}^{\circ}\) & 2.25 \(\pm\) 0.18 \({}^{\circ}\) & 8.51 \(\pm\) 0.09 \({}^{\circ}\) & 1.559 \(\pm\) 0.23 \({}^{\circ}\) & 26.52 \(\pm\) 0.44 \({}^{\circ}\) & 31.68 \(\pm\) 0.24 \({}^{\circ}\) & 54.25 \(\pm\) 0.30 \({}^{\circ}\) & 58.44 \(\pm\) 0.25 \({}^{\circ}\) \\ HHS & 0.21 \(\pm\) 0.01 \({}^{\circ}\) & 0.62 \(\pm\) 0.04 \({}^{\circ}\) & 0.68 \(\pm\) 0.03 \({}^{\circ}\) & 0.72 \(\pm\) 0.01 \({}^{\circ}\) & 0.76 \(\pm\) 0.05 \({}^{\circ}\) & 0.79 \(\pm\) 0.03 \({}^{\circ}\) & 0.81 \(\pm\) 0.06 \({}^{\circ}\) & 0.85 \(\pm\) 0.04 \({}^{\circ}\) \\ TYR & 1.07 \(\pm\) 0.06 \({}^{\circ}\) & 1.58 \(\pm\) 0.06 \({}^{\circ}\) & 1.91 \(\pm\) 0.07 \({}^{\circ}\) & 4.51 \(\pm\) 0.08 \({}^{\circ}\) & 7.01 \(\pm\) 0.09 \({}^{\circ}\) & 8.96 \(\pm\) 0.05 \({}^{\circ}\) & 39.69 \(\pm\) 0.07 \({}^{\circ}\) & 100.06 \(\pm\) 0.05 \({}^{\circ}\) \\ SPD & 2.55 \(\pm\) 0.06 \({}^{\circ}\) & 3.11 \(\pm\) 0.09 \({}^{\circ}\) & 5.60 \(\pm\) 0.02 \({}^{\circ}\) & 6.56 \(\pm\) 0.02 \({}^{\circ}\) & 11.53 \(\pm\) 0.11 \({}^{\circ}\) & 7.86 \(\pm\) 0.04 \({}^{\circ}\) & 4.33 \(\pm\) 0.23 \({}^{\circ}\) & 3.20 \(\pm\) 0.16 \({}^{\circ}\) \\ SPE & 4.15 \(\pm\) 0.12 \({}^{\circ}\) & 5.32 \(\pm\) 0.26 \({}^{\circ}\) & 6.43 \(\pm\) 0.35 \({}^{\circ}\) & 7.82 \(\pm\) 0.67 \({}^{\circ}\) & 18.21 \(\pm\) 0.17 \({}^{\circ}\) & 12.11 \(\pm\) 0.09 \({}^{\circ}\) & 73.2 \(\pm\) 0.26 \({}^{\circ}\) & 4.12 \(\pm\) 0.10 \({}^{\circ}\) \\ \hline \hline \end{tabular} Note: TRP: Tyrumtime; PEA: Phenethylamine; PUT: Putrescine; CAD: cadaverine; HIS: Histamine; YTR: Tyramine; SPD: Spermidine; SPE: Spermine. The same lowercase letter in the same column indicates that there is no difference in color between samples at different storage times (\(p>0.05\)).  
\end{table}
Table 2: Change in BA contents in samples during storage at 4 \({}^{\circ}\)C.  
shelf life of aerobic refrigerated fillets may be 8 days in terms of microbial count. Days 1, 5, 9, 13 were selected for subsequent experimental analysis.