## 4 Discussion  
We have used a diaphanization technique to evaluate the quantity of SLC filled with two root canal sealers with continuous wave. There are numerous clearing techniques, but the one proposed by [PERSON] and [PERSON] [31] is still valid today because of the speed and ease of carrying it out. The clearing technique is a simple method that allows the internal anatomy of the teeth to be observed in three dimensions without requiring specialized tools. Historically, it has been used in combination with Chinese inkjet to observe the natural anatomy of the lateral canal system [5; 31; 36]. It was widely used in studies on simulated lateral canals (SLC) [28; 34; 37; 38], or natural ones [25; 39]. Other authors to study SLC base their work on augmented radiographs such as [PERSON] et al. [40], or they perform cross sections that must be very precise in the axis of the lateral canals, before submerging the sections in resin before their observation, such as [PERSON] et al. [41]. It can be combined with the observation of microfiltration after filling the teeth, such as [PERSON] et al. [37]. Despite having other study techniques, such as micro-CT and FEA, diaphanization presents the possibility of creating SLC. The way to create simulated lateral canals (SLC) differs greatly between authors. [PERSON] et al. prepare the lateral canals in the teeth after cutting the crowns, because they are not decalcified and, due to their normal rigidity, they can be radiographically verified with a #08 K-file inserted in the lateral canal that communicates with the principal canal [28]. [PERSON] et al. prepare the lateral canals with a # 15 rotary file [41]. [PERSON] et al. reported that they create the SLC with cylindrical burs of 0.1 mm diameter at a machining center [37]. [PERSON] et al. [40] used a rotary file #15 adapted to a low-speed hand piece. [PERSON] et al. intend to standardize a new protocol to create SLC with a 60-micrometer diameter, they do so with a #06 K-file, from which they have cut the apical 5- or 8-mm decalcified teeth. They then leave the files on the  
Figure 6: Proportion of apical branches in each root canal sealer group. Bar chart. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & & & \multicolumn{4}{c}{**Root Canal Sealer**} \\ \cline{4-6}  & & \multirow{2}{*}{**None**} & **Biorrot** & **GutaFlow** & **Total** \\  & & & **RCS** & **Bioseal** & **Total** \\ \hline \multirow{4}{*}{**Apical branching**} & No apical branching & Count & 5 & 18 & 16 & 39 \\  & branching & \% within root canal sealer used & 100 & 85.7 & 72.7 & 81.3 \\  & \multirow{2}{*}{Apical branching} & Count & 0 & 3 & 6 & 9 \\  & & \% within root canal sealer used & 0 & 14.3 & 27.3 & 18.8 \\ \hline \multirow{2}{*}{**TOTAL**} & Count & 5 & 21 & 22 & 48 \\  & & \% within root canal sealer used & 100 & 100 & 100 & 100 \\ \hline \hline \end{tabular}
\end{table} 
Table 9: Proportion of apical branches in each root canal sealer group. t teeth during their hardening in methyl salicylate [38; 42]. They do not explain why, but we assume it was so that as the teeth harden and shrink during the clearing process, the SLC are not obliterated. Then they seal, allowing them to check the patency of the SLC and its communication with the main canal. [PERSON] et al. [34] used the same method as this work regarding the creation of lateral canals: after the preparation of the teeth, they are decalcified until reaching a rubbery texture. They insert #8 and 10 K-files into the lingual and buccal surfaces to create the lateral canals. The differences in the heights of the lateral canals are 2.5 and 8 mm of the working length like [PERSON] et al. ; in this study, lateral canals were created in the teeth once they had been decalcified, with a #10 K-file. It is clear to us that the manner and type of file used to create simulated lateral canals (SLC) influences the success of the sealing. That makes comparisons between studies difficult because few studies simulate the proper size of a lateral canal. Most studies make SLC wider [38].