## 3 Results
### Identification of Bacteria  
FASTA formats were examined with the BLASTn software database. Scores, percent identity, and query coverage were used to identify the isolated strain, requiring greater than 96% to define these strains as: _Bacillus_ sp., _Paenibacillus_ sp., and _Enterobacter aerogenes_ for S1, S2, and S3, respectively (homology exceeding 97%). The sequence of these strains was registered with GenBank under accession numbers JX317684, KP189376 and JQ682634, respectively, for _Bacillus_ sp., _Paenibacillus_ sp. and _Enterobacter aerogenes_. Three neighboring trees (phylogenetic trees) of these strains were constructed by comparing the 16S rRNA gene homologies with existing data (Figure 5a-c). These phylogenetic trees revealed that S1, S2, and S3 were strongly associated with _Bacillus_ sp., _Paenibacillus_ sp. and _Enterobacter aerogenes_, respectively. Figure 5: Phylogenetic tree using 16S rRNA gene sequence of isolates and other reference sequences (**(a)** S1, **(b)** S2, **(c)** S3, numbers indicate branch lengths). \begin{table}
\begin{tabular}{c|c|c|c|c|c|c|} \hline  & **TOC** & **TKN** & **C/N** & **Temperature** & _Bacillus_ sp.** & _Paenibacillus_ & _Enterobacter aerogenes_ & **Consortium** \\ \hline TOC & 1 & & & & & & \\ TKN & \(-\)0.913 & 1 & & & & & \\ C/N & 0.888 & \(-\)0.989 & 1 & & & & \\ Temperature & \(-\)0.554 & 0.607 & \(-\)0.667 & 1 & & & \\ _Bacillus_ sp. & \(-\)0.606 & 0.606 & \(-\)0.616 & 0.726 & 1 & \\ _Paenibacillus_ & \(-\)0.660 & 0.596 & \(-\)0.585 & 0.755 & 0.949 & 1 \\ _Enterobacter aerogenes_ & \(-\)0.799 & 0.719 & \(-\)0.691 & 0.764 & 0.922 & 0.925 \\ Consortium & \(-\)0.779 & 0.717 & \(-\)0.727 & 0.862 & 0.932 & 0.964 & 0.920 \\ \hline \end{tabular}
\end{table} 
Table 3: Pearson correlation heatmap of the relationship between physical-chemical variables, bacteria as well as the consortium. In this respect, it has been proven that species belonging to _Bacillus_ sp. are able to use a broad variety of organic matter, in particular resistant substances such as hemicellulose and lignocellulosic compounds, as a valuable carbon and energy supply [27], which explains the improved results achieved in this study. Several authors identify Bacillus as a pioneer in boosting the decomposition of lignocellulosic feedstock [27; 28]. Its ability to break down these recalcitrant molecules is mainly due to its enzymatic mechanisms, such as cellulase or other mechanisms [27; 29]. Furthermore, [PERSON] et al. [30] described how the addition of _Bacillus_ to the bioaugmentation of a mixture of wheat and cattle manure during composting improved the breakdown of lignocellulosic and the fertility of the compost; they revealed that the _Bacillus_ used the lignocellulosic for its own proliferation, while simultaneously generating monomers, such as amino acids, and reducing sugars. These molecules could be used as precursors and could be polymerized into humic substances during the humification process. Recently, [PERSON] et al. [27] demonstrated that Bacillus had a considerable impact on the acceleration of humification. Regarding _Paenibacillus_, extensive research has highlighted its outstanding potential in the decomposition of lignocellulosic compounds [23; 31; 32; 33; 34]. _Penibacillus_ is an ancestral member of the _Bacillus_ genus, known for production of a variety of enzymes, especially amylases, oxygenases, cellulases, hemicellulases, dehydrogenases, lipases, lignin-modifying enzymes, pectinases, etc. [31; 35]. Furthermore, [PERSON] et al. [36], have identified several enzymes which hydrolyse polymers such as cellulose, hemicellulose and/or lignin. Further studies have revealed that this ability may be predominantly due to the presence of specific enzymes directly linked in the depolymerisation of these components, and these findings have been confirmed by NGS [22], which explains the high degradation rate by _Paenibacillus_ sp. In fact, several researchers used _Paenibacillus_ sp. for bioremediation [31; 33; 37]. In this regard, [PERSON] et al.