## 4 Discussion  
In recent years, studying biological activities with compounds isolated from plants has gained importance. It is valuable to discover new drug molecules and to conduct biological, pharmacological, clinical and toxicological studies on these molecules. Molecular docking studies of drug molecules have also gained in importance recently. For these reasons, we aimed to isolate compounds from _S. lavandulifolia_ to test various biological activities and perform molecular docking studies of these compounds. In this study, two flavone glycosides were obtained from _S. lavandulifolia_ ethyl acetate extract by chromatographic methods. With the results of \({}^{1}\)H NMR, \({}^{13}\)C NMR and literature comparison, it was determined that the substances were apigenin-7-O-glucose (C1) and luteolin-7-O glucoside (C2). Then, TYR-, AChE-, and BuChE-inhibitory activities and antimicrobial activities of isolated C1 and C2 were assessed. The AChE-inhibitory effect of C2 was found to be significant. Therefore, molecular docking studies were performed to examine the interactions between the C2 molecule and the AChE enzyme. Compound C1 is a flavone glycoside. Its molecular formula is C\({}_{21}\)H\({}_{20}\)O\({}_{10}\). According to the \({}^{1}\)H-NMR spectrum, proton signals 2\({}^{\prime}\)-6\({}^{\prime}\) (d), 3\({}^{\prime}\)-5\({}^{\prime}\) (d), 3 (s), 8 (d), and 6 (d) were observed at 8.04, 7.11, 6.94, 6.84 and 6.44 ppm. While the anomeric proton signal (H-1\({}^{\prime}\), d) was observed at 5.06 ppm, the signals of other sugar protons were observed in the range of 3.16-3.75 (m) ppm. The signal for the 1\({}^{\prime\prime}\) carbon appeared at 100.3 ppm in the \({}^{13}\)C-NMR spectrum. It has been supported by literature that the C1 molecule is apigenin-7-glucose with 21 carbons [29]. Compound C2 is a flavone glycoside. Its molecular formula is C\({}_{21}\)H\({}_{20}\)O\({}_{11}\). According to the \({}^{1}\)H-NMR spectrum, proton signals 6\({}^{\prime}\) (dd), 2\({}^{\prime}\) (d), 5\({}^{\prime}\) (d), 8 (d), 3 (s), and 6 (d) were observed at 7.43, 7.40, 6.88, 6.77, 6.74, and 6.42 ppm. While the anomeric proton signal (H-1\({}^{\prime\prime}\), d) was observed at 5.06 ppm, the signals of other sugar protons were observed in the range of 3.14-3.70 (m) ppm. The signal for the 1\({}^{\prime\prime}\) carbon appeared at 100.5 ppm in the \({}^{13}\)C-NMR spectrum. It has been supported by literature that the C2 molecule is luteolin-7-glucose with 21 carbons [30]. Antibiotic resistance is an important problem that causes prolonged hospitalization, increased treatment costs and increased mortality rates. Therefore, the discovery of new, reliable and inexpensive antimicrobials has gained importance [15; 16]. Antimicrobial activity assays were carried out by microdilution method against some important pathogen bacteria (_E. coli, K. pneumoniae, P. mirabilis, P. aeruginosa, S. aureus, and E. faecalis_) by comparison with sulfararazine in this study. C1 and C2 showed the strongest effect against _S. aureus_ (MIC value 3.12 ug/mL). In general, it was determined that the compounds showed better activity against Gram-positive bacteria. C1 was found to be effective against _E. coli, K. pneumoniae, S. aureus_, and _E. faecalis_. It was especially effective against _S. aureus_ and _E. faecalis_. C1 was observed to be moreeffective than sulfararazine against _S. aureus_ and _K. pneumoniae_. In previous literature, it was reported that C1 inhibited the growth of _P. vulgaris_, _P. mirabilis_, _K. pneumoniae_, _K. oxytocin_, _M. morgani_, _Streptococcus_ sp., _Enterobacter_ sp., _Seratia_ sp. and the fungi _Candida albicans_. The median diameter of the inhibition zone increased proportionally with the increase in substance concentration [31]. In another study, [PERSON] et al. indicated that C1 is especially effective against _Bacillus subtilis_[32]. Although C1 did not show any activity against some microorganisms, it showed a potentially significant effect against some of them. The literature also supports this.