## 3 Results
As expected, when the SMF parameter approaches the GMF level, the differences become insignificant (\(p>0.05\)) and amount to about 1-7% (right parts of Figure 3a-c); when the SMF is set to 10 nT or 200 nT, and, therefore, differs significantly from the GMF, one can observe significant differences around 11-35% (\(p<0.05\)) (left parts of Figure 3a-c and statistics Figure 3e). An additional control experiment was carried out with a water sample pre-treated with the CMF, but was subjected to dilution and intensive shaking procedures outside the hypomagnetic device, and away (at a distance of 2 m) from ferromagnetic structures (preparation of the test sample at the GMF conditions after pre-incubation of water in the CMF). The experiment revealed similar changes in the activity detected in samples that were located on an magnetic rod, but outside the hypomagnetic device (see Figure 1). For example, for the C50 dilution the activity increased by 128.4 \(\pm\) 11.1% (\(p=0.0047\)) compared to the control (water without any magnetic pre-incubation). It was also shown that the preparation of water dilutions (C12, C30, or C50) in different SMF conditions lead to different results. The differences were most significant in the following groups of samples:  
* C12: 10 nT and 20,000 nT (\(p=0.018\) *),
* C30: 550 nT and 5000 nT (\(p=0.036\) *),
* C50: 900 nT and 200 nT (\(p=0.045\) *); 900 nT and 5500 nT (\(p<0.001\) *); 900 nT and 2500 nT (\(p=0.031\) *). *--statistical comparison of \"all-against-all\" adjusted for the multiplicity of comparison (Holm correction). Thus, the results of testing the biophysical activity of serial dilutions prepared with vigorous shaking of water pre-exposed to a certain CMF field (SMF of 60 \(\upmu\)T; AMF of 0.05 \(\upmu\)T, 12.6 Hz, exposure time of 1.5 h) showed a stable stimulating effect on ROS production by neutrophils when these procedures were performed under conditions of the GMF or artificially created magnetic fields (approximately above 350-550 nT). Parameters of the SMF That Regulate the Ability of HDs of Water to Affect ROS Production by Neutrophils  
The effect of high dilution technology is more pronounced only when the initial substance concentration is minimal. After some threshold (number of dilutions), the physico-chemical effects reach a plateau and do not change regardless of the number of additional technological procedures performed. For example, it has been shown that when serial dilutions are prepared, reactive oxygen species are formed, the concentration of which actually reaches a plateau after approximately six dilutions [3]. The formation of ROS in serial dilutions has already been explained theoretically when creating a physical model where changes in the frequency of vibrations can provide an additional occupancy of the singlet energy level of molecular oxygen. This model explains the process of overcoming the quantum exclusion principle in the transition of oxygen from a triplet to a singlet state [17]. However, despite the molecular and theoretical explanation of the processes occurring when performing serial dilution technology, it remained unclear whether the biophysical properties of HDs (i.e., the ability to affect ROS production by neutrophils) could depend on the number of dilution steps, as well as on the SMF parameters under which HDs were prepared. At the next stage, we investigated the activity of HD samples at different levels of dilution (C12, C30, and C50) using luminol-enhanced cellular chemiluminescence. The results are presented in Figure 4 and Table A1. For all tested water dilutions (C12, C30, and C50 prepared under the CMF conditions with a different SMF component (from 10 nT to 20,000 nT)), the differences between the samples varied.