Thus, even if the limit values defined according to an ACS, such as the EBC, are adhered to, residual risk remains that adverse effects result from the use of BC, especially when used in the vicinity of sensitive habitats, such as water bodies or nature reserves. Concerning these arguments, the harmlessness (respectively the quality) of BC cannot be assessed solely by analyzing physicochemical parameters. Toxicity tests permit an evaluation of the degree to which a chemical substance has an adverse effect in live organisms, either acute or chronic [18]. We hypothesize that the aforementioned toxicological risk of BC used as a soil amendment can be proved with the help of aquatic invertebrate toxicity tests. Toxicity testing in which live organisms, such as aquatic invertebrates, are exposed to contaminants under laboratory conditions, has become a powerful tool in the past years for organisms in marine, estuarine, and freshwater environments [19]. The purpose of toxicity testing is to obtain appropriate insight, which will acquaint decision makers and practitioners about the levels of toxicity and the associated risk created by anthropogenic activities in ecosystems [20]. Eco-toxicological tests provide a substantial tool to assess the toxicity potential of a complex mixture of toxicants typically found in BCs. The toxicity assessment of chemical cocktails and organic contaminants is a growing necessity since single dangerous substances are rarely existent in the environment of anthropogenic activities. Aquatic invertebrates, especially rotifers, are mainly found in freshwater environments but also in moist soil, where they inhabit the thin films of water that are formed around soil particles, which is known as interstitial water [21]. Some aquatic invertebrates, instead of living in particle-free water, prefer to dwell in planktonic, periphytic, and benthic ecosystems, where food availability such as bacteria, eukaryotic cells, algae, and detritus are abundant. Pore or interstitial water in soils is the most bioavailable material for aquatic organisms, whereas fertilized eggs in parched environments become dormant, are able to overwinter, and survive drought unscathed for years [22; 23; 24]. This is why several aquatic invertebrate species are most likely adequate as a suitable bioassay approach to examine the toxicity potential of BC application in the soil, as invertebrates are ubiquitous and the soil is a continuously interacting and cross-biocoenotic ecotope. Most common toxic substances tested with aquatic invertebrates, especially rotifers and cladocerans, comprise natural toxins, pesticides, and heavy metals [25]. The biota contamination by such elements deserves attention, because of cumulative effects within trophic networks [26]. Transportation of heavy metals through trophic networks often commences with the assimilation of these by bacteria and protists [27]. Various heavy metals and organic pollutants, such as antifouling agents, pesticides, PAHs, and PCBs, which are traceable in BCs, have shown reproducible biological responses when tested in rotifers [28]. However, apart from this work, no data obtained by standard acute toxicity tests is available on the susceptibility of aquatic invertebrate species to different toxicants detected in BCs. Most of the acute toxicity tests with aquatic invertebrates measure mortality after an exposure period of 24 or 48 h. These tests have standardized protocols, approved, for example, by the American Society for Testing and Materials (ASTM). Perhaps the most accepted test worldwide is the 48 h acute test using _Daphnia magna_ [PERSON] [29; 30]. Although Mexico has embraced the _D. magna_ test, this European cladoceran species has never been found in Mexican reservoirs nor terrestrial water bodies [31].