## 3 Results and Discussion
### Antibacterial and Antifungal Activity of Bacillus amyloliquefaciens \(\mathrm{M}\)
\begin{table}
\begin{tabular}{c c c c c} \hline \hline \multicolumn{1}{c}{\multirow{2}{*}{_Basillus amyloidiquefaciens_}} & \multicolumn{4}{c}{**Test Microorganisms**} \\ \cline{3-5}  & **Static Cultivation** & \multirow{2}{*}{_Asppergillus niger_**} & _Aspegillus_ & _Aspegillus_ \\
37 \({}^{\circ}\)C & & **ATCC 1015,** & _nawamori_ & **ATCC** \\
24 h & & \(3\times 10^{5}\) cfu/cm\({}^{3}\) & \(1.2\times 10^{5}\) & \(1.12\times 10^{5}\) \\  & & & **cfu/cm\({}^{3}\)** & **cfu/cm\({}^{3}\)** \\ \hline \multirow{3}{*}{\begin{tabular}{c} LBG broth \\ pH = 4.5 \\ \end{tabular} } & ASN & \(18.17\pm 0.24\) & \(10.17\pm 0.24\) & \(25.33\pm 0.47\) \\ \cline{2-5}  & CS & \(20.33\pm 0.47\) & \(12.17\pm 0.24\) & \(25.67\pm 0.47\) \\ \cline{2-5}  & B & \(15.50\pm 0.41\) & \(15.50\pm 0.41\) & \(30.17\pm 0.24\) \\ \hline \multirow{3}{*}{\begin{tabular}{c} LBG broth \\ pH = 5 \\ \end{tabular} } & ASN & \(30.50\pm 0.41\) & \(30.50\pm 0.41\) & \(37.17\pm 0.24\) \\ \cline{2-5}  & CS & \(32.33\pm 0.47\) & \(34.50\pm 0.41\) & \(37.33\pm 0.47\) \\ \cline{2-5}  & B & \(32.67\pm 0.47\) & \(35.33\pm 0.47\) & \(35.50\pm 0.41\) \\ \hline \multirow{3}{*}{\begin{tabular}{c} LBG broth \\ pH = 6 \\ \end{tabular} } & ASN & \(35.33\pm 0.47\) & \(28.17\pm 0.24\) & \(35.17\pm 0.24\) \\ \cline{2-5}  & CS & \(40.67\pm 0.47\) & \(32.50\pm 0.41\) & \(40.33\pm 0.47\) \\ \cline{2-5}  & B & \(40.33\pm 0.47\) & \(33.33\pm 0.47\) & \(43.33\pm 0.47\) \\ \hline \multirow{3}{*}{\begin{tabular}{c} LBG broth \\ pH = 7 \\ \end{tabular} } & ASN & \(30.50\pm 0.41\) & \(30.17\pm 0.24\) & \(37.67\pm 0.47\) \\ \cline{2-5}  & CS & \(35.17\pm 0.24\) & \(32.33\pm 0.47\) & \(37.50\pm 0.41\) \\ \cline{2-5}  & B & \(37.33\pm 0.47\) & \(43.33\pm 0.47\) & \(43.50\pm 0.41\) \\ \hline \multirow{3}{*}{\begin{tabular}{c} LBG broth \\ pH = 8 \\ \end{tabular} } & ASN & \(35.17\pm 0.24\) & \(35.50\pm 0.41\) & \(37.50\pm 0.41\) \\ \cline{2-5}  & CS & \(37.67\pm 0.47\) & \(40.17\pm 0.24\) & \(35.33\pm 0.47\) \\ \cline{2-5}  & B & \(34.33\pm 0.47\) & \(37.17\pm 0.24\) & \(40.67\pm 0.47\) \\ \hline \multirow{3}{*}{\begin{tabular}{c} MPB broth \\ pH = 4.5 \\ \end{tabular} } & ASN & \(30.17\pm 0.24\) & \(34.33\pm 0.47\) & \(35.33\pm 0.47\) \\ \cline{2-5}  & CS & \(37.17\pm 0.24\) & \(32.17\pm 0.24\) & \(34.67\pm 0.47\) \\ \cline{2-5}  & B & \(32.33\pm 0.47\) & \(34.67\pm 0.47\) & \(39.17\pm 0.24\) \\ \hline \multirow{3}{*}{\begin{tabular}{c} MPB broth \\ pH = 5 \\ \end{tabular} } & ASN & \(30.33\pm 0.47\) & \(34.33\pm 0.47\) & \(35.17\pm 0.24\) \\ \cline{2-5}  & CS & \(37.17\pm 0.24\) & \(40.17\pm 0.24\) & \(34.67\pm 0.47\) \\ \cline{2-5}  & B & \(37.50\pm 0.41\) & \(35.50\pm 0.41\) & \(37.33\pm 0.47\) \\ \hline \multirow{3}{*}{\begin{tabular}{c} MPB broth \\ pH = 6 \\ \end{tabular} } & ASN & \(32.17\pm 0.24\) & \(32.33\pm 0.47\) & \(40.50\pm 0.41\) \\ \cline{2-5}  & CS & \(35.17\pm 0.24\) & \(35.67\pm 0.47\) & \(34.17\pm 0.24\) \\ \cline{2-5}  & B & \(37.50\pm 0.41\) & \(34.33\pm 0.47\) & \(35.33\pm 0.47\) \\ \hline \multirow{3}{*}{\
\begin{tabular}{c} MPB broth \\ pH = 7 \\ \end{tabular} } & ASN & \(35.33\pm 0.47\) & \(30.17\pm 0.24\) & \(34.33\pm 0.47\) \\ \cline{2-5}  & CS & \(33.67\pm 0.47\) & \(39.50\pm 0.41\) & \(25.67\pm 0.47\) \\ \cline{2-5}  & B & \(35.17\pm 0.24\) & \(39.50\pm 0.41\) & \(32.67\pm 0.47\) \\ \hline \multirow{3}{*}{
\begin{tabular}{c} MPB broth \\ pH = 7 \\ \end{tabular} } & ASN & \(30.17\pm 0.24\) & \(30.67\pm 0.47\) & \(42.33\pm 0.47\) \\ \cline{2-5}  & CS & \(30.50\pm 0.41\) & \(37.17\pm 0.24\) & \(35.67\pm 0.47\) \\ \cline{2-5}  & B & \(39.33\pm 0.47\) & \(37.50\pm 0.41\) & \(37.50\pm 0.41\) \\ \hline \hline \end{tabular}
\end{table} 
Table 6: Antiphytopathogenic activity of _B. amyloliquefaciens_ M after static cultivation at 37 \({}^{\circ}\)C.  
The strain exhibited strong antagonism against phytopathogenic fungi. The spectrum of antimicrobial action of the strain in different cultural media was close but not identical. This indicates that the antibiotic substances produced by _B. amyloliquefaciens_ M are probably synthesized in different amounts. _B.