## 4 Discussion  
Skin aging and poor wound healing [24] are more common in menopausal women due to decreased estrogen production, which can impair the structure and function of the skin. HRT can help to counteract these side effects. However, the treatmentmay not be suitable for all menopausal women [13]. YCJ has been utilized in several medicinal applications but not for wound healing or for enhancing skin appearance. We were the first to report in 2006 that ovx rats given YCJ had significantly enhanced wound healing, including less scarring, brighter skin, and smoother hair, when compared to controls. However, these findings were based on a four-week period of gross morphological examination at the time. Later, we discovered that YCJ enhanced wound healing by downregulating the macrophage migration inhibitory factor (MIF) and via Er\(\beta\), rather than Er\(\alpha\)[18]. To investigate these findings further, we conducted the current study, which examined changes in the skin, including its underlying structures, at a microscopic level (both LM and TEM) after rats were given YCJ over ten weeks, a longer time period than the original study. This was performed to simulate postmenopausal women's prolonged consumption in an ovariectomized rat. Even though the particular active components or phenolic groups of YCJ have yet to be determined, flavonoids make up most phytoestrogens with wound-healing activities [25]. As a result, we believe the positive effects of YCJ are attributable to its flavonoid properties. YCJ contains 58 percent beta-sitosterol, as well as additional sterols such as stigmasterrol, stigmastatrienol, spinasterol, and fucosterol, as reported in previous studies [17]. According to [PERSON] et al. [26], beta-sitosterol may act as a sex steroid precursor since its structure is similar to animal cholesterol. Furthermore, the methanol extract of coconuts  
Figure 11: (**A**) Gross morphology of wound healing for the sham-control group (SC), ovariectomized group (OC), and ovariectomized rats injected with estradiol benzoate(EB) 2.5 \(\upmu\)g/kg BW/day group (OE), compared with that of YCJ (**B**) feeding groups (OJ): ovariectomized rats fed with YCJ 10 mL/kg BW/day (OJ10), ovariectomized rats fed with YCJ 20 mL/kg BW/day (OJ20), ovariectomized rats fed with YCJ 40 mL/kg BW/day (OJ40).The most accelerated wound healing and hair growth occurred in the OJ40 group.The number subscript indicates the following: 0 = 1 day after ovariectomy, 1 = 1 week after ovariectomy, 2 = 2 weeks after ovariectomy, 3 = 3 weeks after ovariectomy. displayed an estrogenic effect in rats, due to its high concentration of beta-sitosterol, stigmasterol, and other flavonoids [27]. According to earlier studies [28; 29; 30], coconut extract is abundant in phytohormones like abscisic acid (ABA), auxin, gibberellins (GAs), and other cytokins. In human fibroblast cells, trans-zeatin, one of the most significant cytokines contained in coconuts, has been demonstrated to have anti-aging characteristics [31]. According to this study, the rapid effect of YCJ on wound healing is mainly due to its strong estrogenic action, which increases the manufacture of endogenous estrogens. Under LM and TEM examination, the current study discovered that YCJ increased skin thickness while preserving fibroblast and keratinocyte cell shape and increasing collagen fibril quantities. These findings are consistent with [PERSON]'s [32] study of the effects of soybean (_Glycine max_) extracts on the collagen layer and estrogen receptors in female rats' skin. They discovered that soy isoflavues have positive effects on the skin through mechanisms such as lipid oxidation prevention, fibroblast proliferation stimulation, collagen degradation reduction, and the inhibition of 5-reductase [32]. Experiments with a cohort of postmenopausal women treated with isoflav-rich soy extract for six months resulted in considerable increases in epithelial thickness, the number of elastic and collagen fibers, and the number of blood vessels [33].