## 3 Results
### Cultivar Genotype May Influence Bacterial Community Structure  
Different _Vitis vinifera_ cultivars planted in the same field exhibited distinctly different microbial composition. Noteworthy, PCoA (principal coordinate analysis) revealed one distantly apart cluster composed of Syrah, Cardinal, Soultanina, and Vertzami (Figure 3) diverged by 67% from other cultivars based on Bray-Curtis dissimilarities calculated for the composition of the bacterial communities at the genus level. \begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline \multirow{2}{*}{**Cultivar**} & **Good’s** & \begin{tabular}{c} **OTUs** \\ **Excluding** \\ **Singletons** \\ \end{tabular} & **Chao-1** & \
\begin{tabular}{c} **OTUs** \\ **Including** \\ **Singletons** \\ \end{tabular} & **Shannon-H** & **Simpson-1D** &
\begin{tabular}{c} **Reads** \\ **Obtained** \\ \end{tabular} \\ \hline Limiona & 0.997 & **164** & 422.1 & **266** & 2.85 & 0.891 & 31,399 \\ \hline Voidomatis & 0.997 & 109 & 489.3 & 230 & 1.80 & 0.587 & 38,102 \\ \hline Victoria & 0.992 & 113 & 399.0 & 217 & 2.71 & 0.866 & 12,826 \\ \hline Vertzami & 0.996 & 101 & 453.6 & 207 & 2.38 & 0.808 & 23,965 \\ \hline Karabraimis & 0.996 & 105 & 364.4 & 197 & 2.85 & 0.886 & 24,956 \\ \hline Crimson & 0.995 & 96 & 471.0 & 196 & 2.44 & 0.837 & 19,410 \\ \hline Tsaousi & 0.992 & 96 & 357.9 & 194 & 2.92 & 0.908 & 11,580 \\ \hline Xinomavro & 0.996 & 100 & 424.6 & 192 & 2.71 & 0.884 & 24,394 \\ \hline Malagouzia & 0.997 & 86 & 451.0 & 191 & 2.20 & 0.828 & 31,896 \\ \hline Serifiotiko & 0.988 & 82 & 353.4 & 178 & 2.61 & 0.843 & 7839 \\ \hline Mavrokorakas & 0.983 & 79 & 373.3 & 175 & **3.05** & **0.920** & 5798 \\ \hline Vidiano & 0.983 & 77 & 310.0 & 165 & 2.38 & 0.814 & 5271 \\ \hline Mavrodafni & 0.996 & 84 & 334.2 & 163 & 2.64 & 0.887 & 19,820 \\ \hline Roditis & 0.994 & 88 & 290.6 & 162 & 2.74 & 0.887 & 11,964 \\ \hline Merlot & 0.993 & 80 & 341.0 & 161 & 2.56 & 0.872 & 11,869 \\ \hline Cardinal & 0.997 & 81 & 352.6 & 160 & 1.76 & 0.620 & 25,021 \\ \hline Razzaki & 0.997 & 82 & 303.1 & 157 & 2.21 & 0.794 & 22,208 \\ \hline Italia & 0.994 & 68 & 403.5 & 148 & 2.31 & 0.805 & 14,163 \\ \hline Kotselina & 0.995 & 81 & 274.6 & 148 & 2.74 & 0.898 & 13,264 \\ \hline Soultanina & 0.997 & 67 & 434.3 & 147 & 1.85 & 0.704 & 27,181 \\ \hline Korithiaki & 0.994 & 62 & **543.7** & 147 & 2.11 & 0.748 & 14,552 \\ \hline Moschatolefko & 0.992 & 64 & 379.4 & 147 & 2.28 & 0.823 & 9944 \\ \hline Ralli & 0.989 & 60 & 379.2 & 142 & 2.03 & 0.712 & 7139 \\ \hline Savvatiano & **0.999** & 72 & 246.5 & 137 & 1.23 & 0.452 & **44,328** \\ \hline Kakotrygsi & 0.990 & 53 & 284.8 & 125 & 2.37 & 0.847 & 7182 \\ \hline Assyrtiko & 0.992 & 60 & 255.1 & 120 & 2.42 & 0.852 & 7637 \\ \hline Fraoula & 0.995 & 71 & 188.2 & 119 & 2.34 & 0.802 & 9883 \\ \hline Perlette & 0.988 & 49 & 292.2 & 117 & 2.27 & 0.829 & 5587 \\ \hline Agiorgitiko & 0.993 & 48 & 321.0 & 113 & 2.05 & 0.786 & 9735 \\ \hline Sauvignon Blanc & 0.990 & 49 & 239.0 & 106 & 1.87 & 0.665 & 5480 \\ \hline Riesling & 0.991 & 44 & 410.0 & 105 & 2.11 & 0.783 & 6821 \\ \hline Moschofilero & 0.993 & 50 & 217.8 & 103 & 2.37 & 0.821 & 7154 \\ \hline Mavro & _0.977_ & 51 & 191.4 & 103 & 2.82 & 0.889 & 2301 \\ \hline Syrah & 0.998 & 45 & 193.8 & 102 & 1.21 & 0.537 & 30,409 \\ \hline Robola & 0.997 & 49 & 204.1 & 96 & 1.77 & 0.738 & 15,095 \\ \hline Grenache & 0.987 & 37 & 215.3 & 90 & 2.06 & 0.791 & 4158 \\ \hline Cabernet Sauvignon & 0.993 & 52 & _183.1_ & _88_ & 2.55 & 0.884 & 5188 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: The richness and diversity indices of bacterial communities among the studied grapeyines. The highest values in each column are in bold and lowest in italics. The first two PCoA axes (1 and 2) explain 35% of the total variation (Figure 3). Six groups were separated by hierarchical clustering (Figure 4). Significant differences in the microbial community structure were confirmed by PERMANOVA (\(p<0.001\) and F = 6.617) and ANOSIM (\(p<0.001\) and R = 0.6964) statistical analyses.