## 3 Results
### Biological Control of Fusariumium Species in Growth Chamber Conditions  
Non-inoculated plants and plants inoculated only with the bacterial strains did not show disease symptoms throughout the experiment. Control plants inoculated with the strain FC37 showed higher root weights than plants inoculated with strains Hvs2 and Hvs6 or non-inoculated (Table 1).  
The strain FC37 showed the highest level of biocontrol. Asparagus plants coincoulated with _F. proliferation_ isolates Fp3 and Fp86, respectively, and strain FC37 reduced the plant mortality by 58 and 63% with respect to the control inoculated only with the fungal isolates. In addition, the weight of shoots and roots were significantly higher, and the severity of shoot and root symptoms, and the AUDPC were lower in plants coincoulated with _F. proliferation_ isolates and FC37 than in those inoculated only with the fungal isolates (Table 1).  
A similar behavior was observed in plants inoculated with isolates of _F. oxysporum_ f. sp. _asparagi_. The coincocation of the fungal isolate Fo125 with the bacteria FC37 reduced the percentage of dead plants (24%) and produced significant higher root and shoot weights compared with plants inoculated only with the fungal isolate. The severity of roots was similar, although the shoot severity and AUDPC were lower in the coincoulated plants. Fo14 isolate did not produce dead plants, although the coincocation with bacteria FC37 produced a significant increase in the root biomass and a reduction in root symptoms and AUDPC. However, no benefits of strain FC37 were observed in plants inoculated with any of the isolates of F. redolens, except for a slight increase in plant biomass observed in plants coincoulated with Fr104 and FC37, compared with those inoculated only with the fungus (data not shown).  
Figure 2: Dual confrontation experiments showing the inhibition of the fungal mycelial growth of _Fusarium proliferation_ (isolates Fp3 and Fp86), _F. oxysporum_ f. sp. _asparagi_ (isolates Fo14 and Fo125) and _F. redolens_ (isolates Fr7 and Fr104) by the bacterial strains Adb14, Hvs8, FC37, Hvs6 and Hvs2.  
The strain Hvs2 showed an intermediate ability to control _F. proliferation_ and _F. oxysporum_ f. sp. _asparagi_ isolates pathogenic to asparagus, reducing between 26 and 63% the percentage of dead plants, avoiding the loss of plant biomass, and reducing disease symptoms (except for isolate Fo125 of _F. oxysporum_ f. sp. _asparagi_) in plants coincoulated with this bacteria and the respective Fusarium isolates (Table 1). No benefits of Hvs2 were observed in plants inoculated with any of the isolates of _F. redolens_.  
The strain Hvs6 showed a lower ability to control _F. proliferation_ FP3 and FP86 isolates, reducing 14 and 51% the percentage of dead plants, respectively. In addition, the coinoculation of Hvs6 and FP86 isolate avoided the loss of plant biomass and reduced the shoot and root disease symptoms, although to a lesser extent than FC37 and Hvs2 (Table 1). No beneficial effect of Hvs6 was observed on plants inoculated with _F. oxysporum_ and _F. redolens_ isolates.  
Fungal isolates were reisolated from the roots of all inoculated plants although the percentage of fungal recovery was lower in plants coincoulated with any bacterial strain (data not shown).