## 2 Results and Discussion
### GC-MS Analysis of the Volatile Compounds Obtained Using \(Hd\) and HS-SPME  
The EOs obtained using the HD method were subjected to quantitative and qualitative analysis via GC-MS and the results are presented in Table 1. \begin{table}
\begin{tabular}{c c c c c c c c c c} \hline \hline \multirow{2}{*}{**No.**} & \multirow{2}{*}{**Compound**} & \multirow{2}{*}{**RI**} & \multicolumn{6}{c}{**Sample Symbol and Content (\%)**} \\ \cline{3-10}  & & & **RI\({}_{\mathbf{L}}\)** & **M1** & **M2** & **M3** & **M4** & **M5** & **M6** & **M7** \\ \hline
1 & \(\alpha\)-Thujene & 930 & 933 & 0.03 & - & - & - & - & - & - \\
2 & \(\alpha\)-Pinene & 940 & 940 & 0.59 & 1.02 & 1.33 & 1.07 & 1.35 & 0.46 & 2.86 \\
3 & Camphene & 957 & 955 & - & - & - & - & - & 0.43 & - \\
4 & Sabinene & 981 & 980 & 0.56 & 0.79 & 1.01 & 0.86 & 0.99 & - & - \\
5 & \(\beta\)-Pinene & 985 & 982 & 0.93 & 1.38 & 6.69 & 1.16 & 1.64 & 0.38 & - \\
6 & \(\beta\)-Myrcne & 994 & 993 & 0.36 & 0.86 & 1.33 & 4.14 & 1.55 & - & - \\
7 & Octam-3-ol & 998 & 996 & 0.16 & 0.16 & 0.42 & - & - & - & - \\
8 & \(\alpha\)-Phellandrene & 1011 & 1006 & - & - & 0.71 & - & - & - & - \\
9 & \(\alpha\)-Terpinene & 1023 & 1024 & 0.06 & - & 0.74 & 0.28 & - & - & - \\
10 & \(p\)-Cymene & 1030 & 1030 & 0.07 & - & 2.94 & - & 0.51 & - & - \\
11 & Limonene & 1035 & 1035 & - & 9.11 & 1.00 & 5.64 & 6.89 & 0.34 & - \\
12 & 1,8-Cineole & 1039 & 1038 & 4.36 & 8.28 & 2.66 & 4.08 & 6.54 & - & 5.11 \\
13 & \((Z)\)-\(\beta\)-Cycymene & 1043 & 1044 & 0.52 & 1.25 & 7.28 & 0.21 & 1.58 & - & - \\
14 & \((E)\)-\(\beta\)-Cycymene & 1054 & 1054 & 0.12 & 0.41 & 1.42 & - & 0.46 & - & - \\
15 & \(\gamma\)-Terpinene & 1065 & 1064 & 0.12 & - & 7.59 & 0.51 & - & - & - \\
16 & \(cis\)-Sabinenhydrate & 1074 & 1074 & 0.96 & 0.35 & 0.11 & 1.76 & - & - & - \\
17 & \(\alpha\)-Terpinolene & 1092 & 1097 & 0.07 & - & 0.12 & - & - & - & - \\
18 & Linalool & 1103 & 1102 & 0.48 & 0.11 & 40.43 & - & 0.56 & - & - \\
19 & Pentyl 3-methylbutanoate & 1110 & 1108 & 0.06 & - & - & - & 0.41 & - & - \\
20 & Octam-3-yl acetate & 1127 & 1124 & - & - & 0.27 & - & - & - & - \\
21 & Isopulegol & 1152 & 1150 & 0.1 & - & - & - & - & - & - \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Volatile component composition (peak area%) obtained using the HD method and their amount in _Mentha_ spp. Figure 1: Essential oil content in analyzed mint samples obtained by HD method: **M1**_Mentha_\(\times\)_piperata_var. _officialis_f. _pallescens_ Camus ‘Swiss’, **M2**_Mentha_\(\times\)_piperita_f. _citrata_ ‘Grapefruit’, **M3**_Mentha_\(\times\)_piperata_f. _citrata_, **M4**_Mentha_\(\times\)_carinthha_a_Host., **M5**_Mentha_\(\times\)_Mentha_\(\times\)_Mentha_\(\times\)_Mengifolia_var. _schimperi_Brio._, and **M7**_Mentha_\(\times\)_Mentha_\(\times\)_Mengifolia_L._, d.w., dried weight; \(n=3\). Menthol is generally recognized as safe (GRAS), and its toxic effects on humans have rarely been reported [12; 13]. Among the analyzed Eos, only the oil from \(M\). \(\times\)_piperita_ var. _officialis_ f. _pallescens_ [PERSON] \"Swiss\" (**M1**) was characterized by \(p\)-menthone as the main compound (41%) and by menthol (28.19%). Another monoterpene often found in taxa of the genus _Mentha_ is linalool, widely applied in the cosmetic, food, and pharmaceutical industries. In an animal toxicology study, it was shown to cause up to a fourfold increase in serum aspartate aminotransferase enzyme levels and a twofold decrease in body weight in animals when fed with 5% linalool. An increase in this enzyme can lead to myocardial necrosis, liver damage, skeletal muscle damage, or pancreatitis [7]. Linalool (40.43%) was the dominant constituent of the EO from \(M\). \(\times\)_piperita_ f. _citrata_ (**M3**), which is consistent with the literature [14]. Carvone can be found quite commonly in the EOs from different mints and has been shown to have multidirectional biological and therapeutic effects including antibacterial, antifungal, antioxidant, anti-inflammatory, and antihypertensive [15]. In \(M\).