## 3 Results
### An Increased Manganese Resistance of CRN/PPN1 Is Realized through PHO-Pathway Genes
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Gene ID**} & \multirow{2}{*}{**Gene Name**} & \multirow{2}{*}{**Gene Function**} & \multicolumn{4}{c}{**Log2 Fold Change**} \\ \cline{4-6}  & & & **CRN, Mn2* & **CRN**/PPN1, Mn2* & **CRN/PPN1 vs. Control** & **CRN, Control** \\ \hline \multicolumn{6}{c}{**Manganese Transporters and Chelators**} \\ \hline YOL122C & _SMF1_ & Cell surface manganese transporter & **0.96** & 0.05 & 0.14 \\ \hline YHR050W & _SMF2_ & ER membrane manganese transporter & **1.34** & **0.82** & 0.30 \\ \hline YGL167C & _PMR1_ & High affinity Ca2*/Mn2* P-type ATPase of Golgi & **1.27** & **0.82** & 0.08 \\ \hline YLR220W & _CCC1_ & Vacabular Fe2*/Mn2* transporter & –0.09 & 0.00 & 0.56 \\ \hline YBR187W & _GDT1P_ & Calcium and manganese transporter of Golgi & **–1.62** & **–0.78** & **–0.87** \\ \hline YBR056W-A & _MNC1_ & Putative manganese chelating CYSTM-family protein & **2.32** & **1.24** & \(-\)0.02 \\ \hline \multicolumn{6}{c}{**Plasma Membrane ATPases**} \\ \hline YGL008C & _PMA1_ & Plasma membrane H*-ATPase & **0.51** & **0.98** & \(-\)0.03 \\ \hline YPL036W & _PMA2_ & Plasma membrane H*-ATPase & **5.85** & **5.90** & 3.34 \\ \hline \multicolumn{6}{c}{**Polyphosphate Hydroplayzing Enzymes**} \\ \hline YDR452W & _PPN1_ & Exoendopolyphosphatase & \(-\)0.27 & **10.67** & **9.30** \\ \hline YHR201C & _PPX1_ & Exopolyphosphatase & \(-\)0.06 & 0.14 & \(-\)0.12 \\ \hline YNL217W & _PPN2_ & Endopolyphosphatase & 0.00 & **–0.58** & **–0.63** \\ \hline YOR163W & _DDP1_ & Diadenosine and dipphosphionid & **–1.00** & **–0.96** & \(-\)0.46 \\ \hline \multicolumn{6}{c}{**Subunits of the VTC Complex Involved in Polyphosphate Biosynthesis**} \\ \hline YER072W & _VTC1_ & Regulatory subunit involved in membrane trafficking and & **–5.48** & **–5.05** & **–2.94** \\ \hline YFL004W & _VTC2_ & Regulatory subunit involved in membrane trafficking and & **–1.59** & **–1.52** & **–0.98** \\ \hline YPL019C & _VTC3_ & vacuolar pyrophosphate accumulation & **–1.18** & **–2.51** & **–2.33** \\ \hline YDR089W & _VTC5_ & — —0.78** & **–0.96** & 0.07 \\ \hline YJL012C & _VTC4_ & Vacular membrane polyphosphate polymerase & **–0.97** & **–1.48** & **–1.90** \\ \hline \multicolumn{6}{c}{**Phosphate Transport**} \\ \hline \multicolumn{6}{c}{**Activates transcription in response to phosphate limitation; function is regulated by phosphate availability} \\ \hline YFR034C & _PHO4_ & High-affinity inorganic phosphate transporter; also low-affinity manganese transporter, regulated by PhotoP & **–2.29** & **–6.35** & **–5.59** \\ \hline YRL23C & _PHO84_ & Plasma membrane Na*/Pi cotransporter, transcription regulated by PhotoP & **3.52** & 0.18 & **–4.37** \\ \hline YBR296C & _PHO89_ & Plasma membrane Na*/Pi cotransporter, transcription regulated by PhotoP & **3.52** & 0.18 & **–4.37** \\ \hline YCR037C & _PHO87_ & Low-affinity inorganic phosphate transporter & 0.14 & 0.36 & \(-\)0.26 \\ \hline YRL198W & _PHO90_ & Low-affinity phosphate transporter & 0.40 & **0.99** & \(-\)0.02 \\ \hline YNR013C & _PHO91_ & Low-affinity vacuolar phosphate transporter & 0.39 & **0.73** & 0.00 \\ \hline YDR281C & _PHM6_ & Putative transport protein & **––6.0** & **–5.97** & **–3.52** \\ \hline YOL084W & _PHM7_ & Putative transport protein & **12.01** & **11.47** & **8.22** \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Differential expression of the selected genes responsible for manganese transport and belonging to the PHO pathway. The cases of differential expression with FDR-corrected \(p\)-value \(\leq\) 0.05 are marked in bold. Among the genes of polyP hydrolysis enzymes, we should mention \(DDP1\), which is down-regulated in the presence of manganese excess, which accounts for the increase in polyP content in CRN cells. All subunits of the polyP synthesis VTC complex are down-regulated in all cases. Regarding the known phosphate transporters, the genes encoding Pho89 and Pho84 are known to be regulated by the transcription factor Pho4.