# Antibacterial Activity of Crude Extract and Purified Acetogenins from _Amona muricata_ Seeds
## 3 Results and Discussion
### Antibacterial Assays of Crude Extract and Purified Acetogenins
 } & \multicolumn{4}{c}{**Inhibition Halo (mm) Bacteria Gram-Negative**} \\ \cline{2-7}  & \multicolumn{2}{c}{_Aeromonas hydrophulia_} & \multicolumn{2}{c}{_Escherichia coli_} & \multicolumn{2}{c}{_Salmonella cholerasius_} \\ \cline{2-7}  & **CE** & **P-ACGs** & **CE** & **P-ACGs** & **CE** & **P-ACGs** \\ \hline
4000 & \(12.00\pm 0.01\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(11.00\pm 0.01\)\(\mathrm{\SIUnitSymbolMicro y}\) & \(9.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.67\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(11.33\pm 1.15\)\(\mathrm{\SIUnitSymbolMicro x}\) \\
2000 & \(11.00\pm 0.01\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(11.00\pm 0.01\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro y}\) & \(9.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.67\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(11.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) \\
1000 & \(11.67\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(11.67\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) \\
800 & \(12.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(11.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.67\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.67\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(11.00\pm 0.01\)\(\mathrm{\SIUnitSymbolMicro x}\) \\
400 & \(11.67\pm 1.15\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(11.00\pm 0.01\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.67\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.67\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(11.00\pm 0.02\)\(\mathrm{\SIUnitSymbolMicro x}\) \\
200 & \(12.00\pm 0.01\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(11.00\pm 1.00\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.00\pm 0.02\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.67\pm 1.15\)\(\mathrm{\SIUnitSymbolMicro x}\) \\
100 & \(11.67\pm 1.15\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.00\pm 0.02\)\(\mathrm{\SIUnitSymbolMicro y}\) & \(10.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.33\pm 1.15\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.67\pm 1.15\)\(\mathrm{\SIUnitSymbolMicro x}\) \\
50 & \(11.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.00\pm 0.02\)\(\mathrm{\SIUnitSymbolMicro y}\) & \(10.00\pm 0.02\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(8.67\pm 1.15\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.00\pm 0.01\)\(\mathrm{\SIUnitSymbolMicro x}\) \\
25 & \(11.67\pm 1.15\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.00\pm 0.01\)\(\mathrm{\SIUnitSymbolMicro y}\) & \(10.00\pm 0.01\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(8.67\pm 1.15\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.33\pm 1.15\)\(\mathrm{\SIUnitSymbolMicro x}\) \\
12.5 & \(12.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(10.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.00\pm 0.01\)\(\mathrm{\SIUnitSymbolMicro y}\) & \(10.00\pm 0.02\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) & \(9.33\pm 0.58\)\(\mathrm{\SIUnitSymbolMicro x}\) \\ Ampicillin & & & & & & \\
500 \(\mathrm{\SIUnitSymbolMicro g}\)/mL) & & & & & & \\ \hline Distilled water & & & & & & \\ \multicolumn{2}{c}{} & & & & & \\ \multicolumn{2}{c}{} & & & & & \\ \multicolumn{2}{c}{} & & & & & & \\ \multicolumn{2}{c}{} & & & & & & \\ \multicolumn{2}{c}{} & & & & & & \\ \multicolumn{2}{c}{} & & & & & & \\ \multicolumn{2}{c}{} & & & & & & \\ \multicolumn{2}{c}{} & & & & & & \\ \multicolumn{2}{c}{} & & & & & & \\ \multicolumn{2}{c}{} & & & & & & \\ \multicolumn{2}{c}{} & & & & & & \\ \multicolumn{2}{c}{} & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & & \\ \multicolumn{2}{c}{} & & & & & & &The cell envelope of Gram-negative bacteria is a relatively thin wall composed of a single layer of peptidoglycan, lipoproteins, lipopolysaccharide, and the outer membrane, which is crucial as a molecular barrier for stopping the loss of intracellular proteins and restricting access to drugs, especially hydrophobic antibiotics [16; 31].