## 3 Results and Discussion
### Elemental Profile of SN Extracts
\begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Element** & **Accuracy * (\%)** & **Precision * (RSD, \%)** & **Intra-Day** & **Inter-Day** \\  & & & **Precision * (\%)** & **Precision * (\%)** \\ \hline Pb & S\({}_{1}\) (92–106) & S\({}_{1}\) 3.50 & S\({}_{1}\) 3.15 & S\({}_{1}\) 3.46 \\  & S\({}_{2}\) (93–102) & S\({}_{2}\) 2.90 & S\({}_{2}\) 2.78 & S\({}_{2}\) 2.76 \\ \hline Cd & S\({}_{1}\) (94–112) & S\({}_{1}\) 5.80 & S\({}_{1}\) 5.74 & S\({}_{1}\) 5.56 \\  & S\({}_{2}\) (94–102) & S\({}_{2}\) 2.80 & S\({}_{2}\) 2.79 & S\({}_{2}\) 2.66 \\ \hline As & S\({}_{1}\) (100–114) & S\({}_{1}\) 4.70 & S\({}_{1}\) 4.48 & S\({}_{1}\) 4.54 \\  & S\({}_{2}\) (96–105) & S\({}_{2}\) 2.10 & S\({}_{2}\) 3.09 & S\({}_{2}\) 3.10 \\ \hline Cu & S\({}_{1}\) (87–98) & S\({}_{1}\) 3.70 & S\({}_{1}\) 3.71 & S\({}_{1}\) 3.59 \\  & S\({}_{2}\) (99–109) & S\({}_{2}\) 3.40 & S\({}_{2}\) 3.37 & S\({}_{2}\) 3.24 \\ \hline Fe & S\({}_{1}\) (93–104) & S\({}_{1}\) 3.30 & S\({}_{1}\) 3.36 & S\({}_{1}\) 3.31 \\  & S\({}_{2}\) (92–104) & S\({}_{2}\) 3.70 & S\({}_{2}\) 3.63 & S\({}_{2}\) 3.57 \\ \hline Zn & S\({}_{1}\) (88–104) & S\({}_{1}\) 5.90 & S\({}_{1}\) 5.81 & S\({}_{1}\) 5.61 \\  & S\({}_{2}\) (93–106) & S\({}_{2}\) 4.00 & S\({}_{2}\) 3.98 & S\({}_{2}\) 3.84 \\ \hline Mn & S\({}_{1}\) (90–110) & S\({}_{1}\) 5.90 & S\({}_{1}\) 5.90 & S\({}_{1}\) 5.87 \\  & S\({}_{2}\) (94–104) & S\({}_{2}\) 2.50 & S\({}_{2}\) 2.37 & S\({}_{2}\) 2.46 \\ \hline Ni & S\({}_{1}\) (80–90) & S\({}_{1}\) 4.60 & S\({}_{1}\) 4.69 & S\({}_{1}\) 4.50 \\  & S\({}_{2}\) (92–106) & S\({}_{2}\) 6.50 & S\({}_{2}\) 6.51 & S\({}_{2}\) 6.21 \\ \hline Cr & S\({}_{1}\) (87–93) & S\({}_{1}\) 1.90 & S\({}_{1}\) 1.90 & S\({}_{1}\) 1.89 \\  & S\({}_{2}\) (96–101) & S\({}_{2}\) 1.80 & S\({}_{2}\) 1.78 & S\({}_{2}\) 1.70 \\ \hline K & S\({}_{1}\) (99–106) & S\({}_{1}\) 2.60 & S\({}_{1}\) 2.53 & S\({}_{1}\) 2.49 \\  & S\({}_{2}\) (97–104) & S\({}_{2}\) 2.20 & S\({}_{2}\) 2.25 & S\({}_{2}\) 2.15 \\ \hline Na & S\({}_{1}\) (103–108) & S\({}_{1}\) 1.80 & S\({}_{1}\) 1.80 & S\({}_{1}\) 1.75 \\  & S\({}_{2}\) (102–109) & S\({}_{2}\) 1.80 & S\({}_{2}\) 1.58 & S\({}_{2}\) 1.78 \\ \hline Ca & S\({}_{1}\) (94–109) & S\({}_{1}\) 3.80 & S\({}_{1}\) 3.84 & S\({}_{1}\) 3.79 \\  & S\({}_{2}\) (91–103) & S\({}_{2}\) 3.00 & S\({}_{2}\) 2.76 & S\({}_{2}\) 2.94 \\ \hline Mg & S\({}_{1}\) (90–101) & S\({}_{1}\) 3.50 & S\({}_{1}\) 3.49 & S\({}_{1}\) 3.35 \\  & S\({}_{2}\) (93–104) & S\({}_{2}\) 4.10 & S\({}_{2}\) 4.14 & S\({}_{2}\) 3.99 \\ \hline \hline \end{tabular}  
* Accuracy, precision, inter-day, and intra-day precisions for both spiking levels (S\({}_{1}\) and S\({}_{2}\)) are given in the Table, RSD-relative standard deviation.  
\end{table} 
Table 2: Accuracy, precision, intra-day, and inter-day precisions of the applied method. samples. The highest contents of potassium, and magnesium were found in the MAE extract (965.60 mg/L, and 191.99 mg/L, respectively), while MAC was found to be the richest in calcium and natrium (464.24 mg/L, and 90.37 mg/L, respectively). The content of trace elements (microelements) achieved more diversity that in the case of bulk elements. Results showed that highest amounts of iron and zinc were in the UAE extract, while manganese and nickel achieved their maximal yield in the MAE extract. Maximal levels of cooper, chromium, and tin were achieved in UAE. In the case of polluting elements, lead was detected in all four samples, where SE, and UAE contained somewhat higher amount than the MAC, and MAE. Cadmium was detected in a rather trace level in the SE, and UAE extracts. On the other hand, mercury was not detected in any extract sample. Arsenic was quantified only in the SE extract. Possible explanation of arsenic's presence in the SE extract is interference of ethanol during the introduction of the sample into argon plasma. Based on ANOVA, it could be concluded that there was a significant effect (\(p<0.01\)) of the type of extraction on the content of all macro and microelements, except for Ni and Sn.