## 3 Results and Discussion
### Molecular Analysis
In the genetic similarity analysis and dendrogram creation, all four analyzed concentrations were taken into account for BAP and KIN, respectively. The tested regenerants from the medium without phytohormones showed high genetic similarity to the donor plant, reaching a level of 0.84 (Table 6). This allowed us to conclude that in this regeneration variant, the obtained shoots were free from significant somaclonal variations. This level of genetic similarity in a study by [PERSON] et al. [47] indicated that true-to-type regenerants of _Salvia bullyinga_ were obtained. Based on Figure 4, it can be observed that regenerants from the MS \"0\" medium were grouped together with the donor plant. Due to the high value of the genetic similarity of regenerants obtained in the KIN medium, both in relation to DP and MS \"0\", regenerants from the MS + KIN medium were added to the dendrogram as the next ones. Low similarity values were observed between the regenerants from the MS + BAP medium and the donor plant as well as MS \"0\" regenerants (Table 6), as a result of which regenerants from the MS + BAP medium were included last in the formed cluster. The presence of somaclonal variation among regenerants originating from a single donor plant was indicated by [PERSON] et al. [49] and [PERSON] et al. [50]. Due to the genetic heterogeneity of callus tissue, shoots/plants regenerated through it may exhibit variation at the molecular level [47; 51; 52]. The observed low level of genetic similarity regenerants obtained through indirect shoot organogenesis in the medium containing BAP in relation to donor plants was consistent with reports by other authors, such as [PERSON] et al. [53] and [PERSON] et al. [54]. According to some authors [55; 56], BAP, as well as other growth regulators such as 2,4-dichlorophenoxyacy acetic acid (2,4-D) and naphthaleneacetic acid (NAA), may be responsible for the occurrence of variability. Moreover, the balance between cytokins and auxins may influence the process of callus tissue formation and development [57], and the genetic variability obtained through it can be utilized for the selection of desirable traits in breeding programs [58; 59; 60; 61]. However, such variability is undesirable in the commercial-scale multiplication of a wide range of crops. As [PERSON] et al. [24] argued, the genetic stability of plants obtained in vitro depends on the regeneration method applied. In such cases, a method that allows for maintaining high genetic integrity should be employed. According to [PERSON] [48], direct shoot regeneration should ensure that true-to-type regenerants are obtained. As stated by [PERSON] et al. [30], the method of the clonal propagation of plants through adventitious and axillary bud explants is a technique that maintains genetic stability. As regards the analyzed study, direct organogenesis observed on a medium with kinetin resulted in a high genetic similarity, particularly to plants from the MS \"0\" medium, which are a source of explants for cultures grown on the MS + KIN medium. However, as reported by [PERSON] et al. [25], kinetin present in the medium may also contribute to generating variability within somaclones. With respect to the work analyzed, this was reflected in the lower genetic similarity obtained in the MS + KIN medium than the MS \"0\" in relation to DP plants. The reason for such a situation, as indicated by [PERSON] et al. [62], could be attributed to the influence of PGRs that increase the multiplication rate and induce adventitious shoots or existing genetic variation in cultured cells or tissues [31]. \begin{table}
\begin{tabular}{l l l l l} \hline  & **DP** & **MS \"0\"** & **MS + KIN** & **MS + BAP** \\ \hline
**DP** & 1.0 & 0.84 & 0.76 & 0.58 \\
**MS \"0\"** & & 1.0 & 0.80 & 0.47 \\
**MS + KIN** & & & 1.0 & 0.67 \\
**MS + BAP** & & & 1.0 \\ \hline \end{tabular}
\end{table} 
Table 6: Matrix of genetic similarity between regenerants from MS medium with different PGRs and donor plant obtained on the basis of SCoT markers.