## 3 Results and Discussion
### Bacterial Diversity and Community Profiles  
Soil contaminated with myriad of pollutants has become a serious problem across the world. Studies which concentrate on deciphering the microbial community profiles of contaminated soil helps us to design strategies to remediate such sites by targeting the key microbial players in degradation of the particular pollutant [33]. There have been numerous studies in designing reactors for treating textile wastewater which are rich in azo compounds for decreasing the levels of effluents in the environment [13; 14]. In this study, a total of 50 and 54 phyla were detected for DS and ALS respectively (Figure 2). The phyla profiles were different for both DS and ALS. _Saccharibacteria_ (36.4%) were observed to be the most abundant in DS (Figure 2a) whereas _Proteobacteria_ (25.4%) were the abundant ones in ALS (Figure 2b). Negative impact of azo dyes on soil _Proteobacteria_ were previously reported [5]. Reactive Black 5 Direct Red 81 used by [PERSON] et al. [5] found to reduce the _Proteobacteria_ population significantly in soil. The other phyla dominating DS are _Actinobacteria_ (20.02%), _Proteobacteria_ (15.49%), W55 (4.63%), _Acidobacteria_ (4.16%) and _Chloroflexi_ (3.31%). Similarly, the other phyla dominating ALS are _Saccharibacteria_ (15.24%), _Actinobacteria_ (10.03%), _Acidobacteria_ (9.94%), _Plantomycetes_ (8.4%), _Gemmatinondates_ (7.59%), _Chloroflexi_ (6.34%) and _Bacteroidetes_ (3.48%). Different genera of _Proteobacteria_ were isolated from the textile effluents wastewater treatment plants [34], and these _Proteobacteria_ might have been involved in degradation of azo dyes. _Saccharibacteria_ have been observed to be highly active in sludge reduction and dye wastewater treatment plants [14; 35]. Furthermore, _Saccharibacteria_ are regarded as one of the active microbial group in anoxic carbon-based fluidized bed reactor treating coal pyrolysis wastewater which contains abundant phenolic compounds [36]. In another study, it has been reported that _Actinobacterial_ extract [37] and its related enzymes [38] are important in degradation of azo dyes. Hence, the abundance of _Saccharibacteria_ and _Actinobacteria_ in this study can be attributed to the fact that these phyla are responsible for remediating/decolorizing azo dye compounds present in the textile wastewater. _Saccharibacteria_ and _Actinobacteria_ present in azo dye contaminated soil, indicates their important in degradation of soil pollutants. Since the results obtained by pyrosequencing techniques, many of them might be unculturable. In future, selective culturing of _Saccharibacteria_ and _Actinobacteria_ from this azo dye contaminated soils could be the opportunity to obtain efficient strains for remediation of azo dye polluted soils. \begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{2}{*}{**Soil Samples**} & **Number of** & **Chao 1** & **Shanon** & **Inv-Simpson** \\  & **OTUs** & **(Richness)** & **(Diversity)** & **(Diversity)** \\ \hline DS & 8302 & 1303.71 & 5.54 & 51.82 \\ ALS & 12162 & 1472.93 & 6.25 & 158.78 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Microbial diversity estimates of soil samples. Figure 2: Relative abundance of bacterial lineages of (**A**) DS and (**B**) ALS in phylum level. On the other hand, the abundance of _Proteobacteria_ in agricultural soil also corroborates to previous studies [32]. As the agricultural sector uses mostly inorganic NPK fertilizers, which has resulted in increase in the relative abundance of _Proteobacteria_ in agricultural fields [39]. The abundance of _Saccharibacteria_ in agricultural soil can be correlated to the evidence that the dam water used for irrigation of the agricultural soil has resulted in contamination of the field [13,34]. The abundance of _Actinobacteria_ in ALS can be explained by the fact that these bacterial group consists of plethora of plant growth promoters which are predominantly found in agricultural soils [18].