###### Abstract  
In recent work, it was shown that the graminoid plants _Cynodon dactylon_ (_Paceace_), _Cyperus evalutus_ (_Cyperacae_), and _Panicum repens_ (_Paceae_) have an oviposition effect on the malaria vector _Anopheles gamibe_ in olfactometric bioassays. In order to get a view of the diversity of semichenicals present in the environment of the vector during olfactometric trials, in the present work, the volatile profiles of these graminoid plants were analyzed using headspace solid-phase microextraction (HS-SPME) together with gas chromatography-mass spectrometry (GC-MS). In addition, one-way ANOVA comparison of compounds detected in two or more headspace samples are presented in order to provide a basis for comparison of compounds that could constitute a starting point for novel blends of volatile organic compounds to be tested as oviposition attractants. Cynodon dactylon; Cyperus evalutus; Panicum repens; HS-SPME-GC-MS; volatile profile Article  
Chemical Diversity between Three Graminoid Plants Found in Western Kenya Analyzed by Headspace Solid-Phase Microextraction Gas Chromatography-Mass Spectrometry (HS-SPME-GC-MS), _Pants_ **2021**, 10, 2423. [[https://doi.org/10.3390/plants1012423](https://doi.org/10.3390/plants1012423)]([https://doi.org/10.3390/plants1012423](https://doi.org/10.3390/plants1012423))  
1  
The study of the influence of volatile compounds and their behavioral effects on insects is an important aspect of chemical ecology. The development of control strategies against disease vectors, based on the semichenicals available from plants, has, for example, led to advances in new pest control concepts. One goal is to decrease the spread of diseases associated with certain species of insects. An example of such a severe problem is the spread of malaria in sub-Saharan Africa, which has declined [1] when compared to the goals set by the World Health Organization (WHO) in their Global Technical Strategy for Malaria (GTS) report from 2016 [2]. As of now, long-lasting insecticide nets (LLINs) and indoor residual spray (IRS) are the most commonly employed techniques to minimize the infection rate of malaria. However, these methods are limited to indoor use, and it has been shown that the effectiveness has subsided due to a decrease in usage [1]. Increased resistance in certain _Anopheles_ mosquitoes towards the utilized insecticides has also been reported [3]. One of the main malaria vectors is the _Anopheles gamibe_ mosquito, whose host-seeking [4, 5] and oviposition behaviors [4, 5, 6] are impacted by the semichenicals in its environment. In order to obtain new methods of vector control, the regulation of oviposition using semichenicals has thus been suggested, focusing on outdoor settings, such as \"lure-and-kill\", that can provide an alternative to the indoor methods [7, 8]. The challenge in the development of these novel outdoor-focused control techniques is the identification of volatile organic compounds (VOCs) that could be used to influence the malaria vectors' choice of egg-laying site. To be able to determine the volatile compounds that elicit this behavior from the target disease vectors, the presence of the VOCs must be established. With the knowledge of what VOCs are present in the chemical environment of the vector as oviposition occurs,one can aim to relate the VOCs to the behavior of the vector. In addition to establishing VOCs in the chemical environment, the sources of these VOCs should be determined. In a study by [PERSON] et al., a correlation between graminoid plants and the occurrence of _A. gambiae_ instars was shown. It was determined that the choice of egg laying sites is influenced by the presence of water and certain graminoid plants. From this study, it could also be seen that the plants associated with the occurrence of the instars of _A. gambiae_ are _Cyperus rotundus_ (Nut grass) and _Cyperus exaltatus_ (Exaltatus Grass) from the Cyperaceae family, as well as _Cynodon dactylon_ (Bermuda Grass) and _Panicum repens_ (Torpedo Grass) from the Poaceae family [9].