## 4 Discussion
The capacity of the extract to scavenge free radicals using the DPPH method revealed that myrrh suppressed free radicals in a concentration-dependent way. Higher levels of polyphenolic chemicals may be responsible for the considerable reduction of free radicals [42]. Myrrh extracts have a high level of H2O2 scavenging action. It is generally known that the breakdown of H2O2 produces hydroxyl free radicals in the blood. Hydroxyl radicals are produced by the breakdown of H2O2 and the activation of neutrophils, and are thought to play a key role in the oxidative stress linked to acute lung injury [43]. Antioxidants have been demonstrated to reduce the activation of NF-kB by H2O2 as well as all other inducers [44]. DNA damage and the activation of oncogenes such as K-ras and C-Raf-1 have both been linked to hydroxyl free radicals [45]. Our findings show that myrrh extracts can prevent heat-mediated denaturation of albumin and egg albumin, which have been linked to rheumatoid arthritis. The ability to inhibit albumin denaturation was evaluated to establish the basis of myrrh extract's anti-inflammatory activity. As a result, it may be stated that myrrh extract has significant anti-inflammatory properties. Furthermore, protein denaturation results in the production of autoantibodies [46]. The presence of medicinally active flavonoids in myrrh extract may account for this capacity [47]. Furthermore, anti-proteinase activity is thought to protect against tissue damage caused by proteinase [39; 48]. Additionally, the deposition of auto-antigens in the synovial joints is likely to cause joint inflammation. The activation of matrix metalloproteinases by ROS is considered to be involved in severe tissue destruction, such as that found in different arthritic responses of the body [21]. Inhibitors of glycation and AGE production may thus be effective in the prevention and treatment of diabetic complications [2], as well as cancer [49]. In the current study, myrrh extract substantially decreased browning intensity, AGE production, and cross-amyloid formations in a concentration-dependent way, compared to all other research on glycation and AGE formation. It has been previously suggested that antioxidant activity, polyphenic content, and AGEs formation inhibition are linked with each other [39]. As a result, the quantity of polyphenic chemicals with high antioxidant activity may be responsible for myrrh extract's ability to suppress the production of AGEs. Flavonoids and other polyphenic substances have been shown to protect against the development of cancer [50]. We conducted a molecular docking investigation to confirm the molecular defenses of myrrh phytoconstituents against oxidative stress and conformational alterations of anti-oxidant enzymes as well as to link numerous potential compounds of myrrh to special chemical features. This further confirms our hypothesis that myrrh extract may be capable of protecting these enzymes from denaturation caused by glycation, as well as defending from various oxidative stress-mediated health ailments. Furthermore, a molecular docking investigation was conducted between the SARS-CoV-2 Spike protein S and myrrh extract constituents to identify SARS-CoV-2 virus inhibitors, and to validate the concept that Myrrh extract can block SARS-CoV-2 virus' access into human cells due to its ability to bind viral proteins. It is already discussed that spike (S) glycoproteins of the SARS-CoV-2 virus facilitate this virus' entrance into the cell via interaction with ACE2 [31; 51]. From these docking studies, it was seen that the interaction of these myrrh extract constituents and Spike protein S could possibly suggest the inhibitory action of myrrh extract against the SARS-CoV-2 virus. Through in vitro tests, our research supports the therapeutic benefits of myrrh against the pathophysiology of different health ailments. Myrrh extract may be used to reduce oxidative stress linked with glycation-induced denaturation of these antioxidants in diabetic patients.