## 2 Materials and Methods
### Avian Cell Association Assay  
Budgerigar Abdominal Tumor Cells (BATCs) [17] were grown in a complete Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 20% Fetal Bovine Serum (Thermo Fisher Scientific, Waltham, MA, USA). The cells were grown in 75 cm\({}^{2}\) cell culture flasks (CellBIND surface; Corning Inc., Corning,NY, USA) and incubated at 37 \({}^{\circ}\)C under 5% CO\({}_{2}\). The cells were cultured to reach >95% confluence. The confluent cells were seeded into 24-well tissue culture plates (Corning Inc., Corning, NY, USA) containing 1 mL of complete medium and were grown for 48 h to reach >95% confluence (10\({}^{5}\) cells/wells) in the wells. Each strain tested (\(n=24\)) was first grown in MRS broth at 37 \({}^{\circ}\)C for 18 h with shaking in aerobic conditions to reach 10\({}^{8}\) CFU/mL. These bacteria were centrifuged at 4000\(\times\)\(g\) for 10 min at 25 \({}^{\circ}\)C, washed once, and resuspended in PBS. An initial OD\({}_{600}\) reading was taken for each isolate prior to inoculation. The BATC cells in the 24 wells (10\({}^{5}\) cells/wells) were washed three times with PBS. The BATC cells immersed in 1 mL of PBS were then inoculated with 10\({}^{6}\) CFU/mL of each test strain separately and were incubated for 2 h at 37 \({}^{\circ}\)C under 5% CO\({}_{2}\).  
Following incubation, the cells were washed three times with PBS to remove the unattached bacterial cells. The cells were then incubated in 1 mL of 0.1% Triton X-100 for 15 min at 37 \({}^{\circ}\)C under CO\({}_{2}\) for host cell lysis. The cell lysates were then serially diluted in PBS, and appropriate dilutions of lysates were plated on MRS plates and incubated for 48 h at 37 \({}^{\circ}\)C in anaerobic conditions. The plates were counted for CFU/mL determination. To calculate the initial CFU/mL for each isolate, the individual OD\({}_{600}\) reading was inserted into to a calibration curve equation determined for a representative strain from each genus. Isolate ID UMNLJ21 was selected for the _Lactobacillus_ genus: CFU/mL initial = (((\((1\times 10^{8})\times\) OD\({}_{600}\)) \(-\) (5\(\times\) 10\({}^{6}\))). Isolate ID MOF2W3B1T3 was selected for the _Ligilactobacillus_ genus: CFU/mL initial = (((\(2\times 10^{8}\)) \(\times\) OD\({}_{600}\)) \(-\) (4\(\times\) 10\({}^{7}\))). Isolate ID MOF2W3B1C8 was selected for the _Linusilactobacillus_ genus: CFU/mL initial = (((\(7\times 10^{8}\)) \(\times\) OD\({}_{600}\)) \(-\) (1\(\times\) 10\({}^{8}\))). Isolate ID MOF2W1 LC6 was selected for the _Pedicoccus_ genus: CFU/mL initial = (((3\(\times\) 10\({}^{9}\)) \(\times\) OD\({}_{600}\)) \(-\) (1\(\times\) 10\({}^{9}\))). Isolate ID MOF2W2 LT2 was selected for the _Weissella_ genus: CFU/mL initial = (((\(3\times\) 10\({}^{7}\)) \(\times\) OD\({}_{600}\)) \(+\) (132,508)). Percent adherence was calculated by dividing the final CFU/mL by the initial CFU/mL and multiplying by 100 for each replicate. Prior to statistical comparison, outliers were identified and removed. Outliers were defined as a single isolate replicate \(>\) 15% different from all the other replicates of the same isolate. N = 8 outliers were identified and removed from the dataset prior to statistical comparison. The isolates that had outliers removed from their dataset were MOF2W1B3C5, MOF2W1 LC6, MOF2W3B1T2, MOF2W3B5T3, MOF2W3 LC2, MOF2W5B1C1, MOF2W6B4C10, and SNF2W3B2M2.