## 3 Results
### Biochemical Composition  
The biochemical composition of each native microalgae isolate was evaluated. The whole algal biomass compositions of newly isolated strains, MAa1 and MAn1, were summarized in Table 1.  
Proteins and carbohydrates were the most abundant compounds for both studied strains, which could be of interest for several biotechnological applications. As shown in Table 1, _Chlamydomonas_ sp. MA\({}_{\text{n1}}\) strain accumulated a significantly higher quantity of proteins (47.22%), lipids (25.55%), chlorophyll (1.5 mg/L), and phenolic compounds (40.5 mg/mL) than _Navicula_ sp. MA\({}_{\text{n1}}\) strain. Besides, an important amount of carbohydrates (50.83%) has been found in the algal biomass of _Navicula_ sp. MA\({}_{\text{n1}}\) strain.  
Fatty acid profiles were determined for each isolate via fatty acid methyl esters analysis (Table 2).  
Eighteen and twenty fatty acids have been identified in the present study for _Chlamydomonas_ sp. MA\({}_{\text{n1}}\) and _Navicula_ sp. MA\({}_{\text{n1}}\) isolates, respectively. _Chlamydomonas_ sp. MA\({}_{\text{n1}}\) strain, showed the highest portion of polyunsaturated fatty acids (PUFAs) 44.35% with  
\begin{table}
\begin{tabular}{c c c} \hline \hline  & _Chlamydomonas_ sp. MA\({}_{\text{n1}}\) & _Navicula_ sp. MA\({}_{\text{n1}}\) \\ \hline Proteins (\%) & 47.22 \(\pm\) 0.19 & 34.16 \(\pm\) 0.05 \\ Lipids (\%) & 25.55 \(\pm\) 0.2 & 15.00 \(\pm\) 0.1 \\ Carbohydrates (\%) & 27.23 \(\pm\) 0.05 & 50.83 \(\pm\) 0.2 \\ Chlorophyll (mg/L) & 1.5 \(\pm\) 0.3 & 0.95 \(\pm\) 0.05 \\ Phenols (mg/mL) & 40.5 \(\pm\) 0.15 & 11.32 \(\pm\) 0.05 \\ Carotenoid (mg/L) & 3.81 \(\pm\) 0.3 & 4.72 \(\pm\) 0.15 \\ \hline \hline \end{tabular}
\end{table}
Table 1: Microalgae biomass composition.  
\begin{table}
\begin{tabular}{c c c} \hline \hline  & \multicolumn{3}{c}{**Fatty Acid (_Relative Percentage_)**} \\ \hline  & _Chlamydomonas_ Sp. MA\({}_{\text{n1}}\) & _Navicula_ Sp. MA\({}_{\text{n1}}\) \\ \hline C14:0 Myristic acid & 0.69 & 4.96 \\ C15:0 Pentadecylicaid & 0.13 & 1.62 \\ C16:0 Palmitic acid & 23.87 & 10.80 \\ C18:0 Stearic acid & 4.98 & 0.45 \\ C20:0 Arachidic acid & 0.27 & 0.00 \\ C22:0 Behenic acid & 0.41 & 0.15 \\
**Total saturated (\%)** & **30.35** & **17.98** \\ \hline C16:1n-7 Palmitic acid & 1.14 & 43.48 \\ C18:1n-9 Oleic acid & 12.50 & 5.04 \\ C18:1n-7 Vaccenic acid & 10.45 & 2.07 \\ C20:1n-9 Gondoic acid & 1.00 & 0.14 \\ C22:1n-9 Erucic acid & 0.00 & 0.08 \\ C24:1n-9 Nervonic acid & 0.21 & 0.07 \\
**Total monounsaturated (\%)** & **25.30** & **50.88** \\ \hline C18:2n-6 Linoleic acid & 5.82 & 2.91 \\ C18:3n-6 \(\gamma\)-linoleic acid & 0.33 & 1.46 \\ C20:3n-6 Dihomo-\(\gamma\)-linoleic acid & 0.00 & 0.56 \\ C20:4n-6 Arachidic acid & 1.41 & 5.64 \\ C22:2n-6 Docosadonic acid & 0.36 & 0.14 \\ C22:4n-6 Adrenic acid & 0.00 & 0.14 \\
**Total n-6 PUFA (\%)** & **7.92** & **10.85** \\ \hline C18:3n-3 \(\alpha\)-linolenic acid & 30.08 & 0.00 \\ C18:4n-3 Stearidonic acid & 0.00 & 0.05 \\ C20:3n-3 Dihomo-\(\alpha\)-linolenic acid & 0.20 & 0.00 \\ C20:5n-3 Eicosapentaenoic acid & 6.15 & 19.99 \\ C22:6n-3 Docosabexanoic acid & 0.00 & 0.25 \\
**Total n-3 PUFA (\%)** & **36.43** & **20.29** \\ \hline
**Total PUFAs (\%)** & **44.35** & **31.14** \\ \hline
**n-3/n-6** & **4.59** & **1.87** \\ \hline \hline \end{tabular}
\end{table}
Table 2: Fatty acid compositional profiles of lipid extracts from microalgae isolates.  
7.92% of n-6 PUFA and 36.43% of n-3 PUFA. Regarding MA\({}_{\text{n1}}\) isolate, belonging to _Navicula_ genus, MUFAs was the major portion of the total fatty acids (50.88%) with a dominance of palmitoleic acid (C16:In-7) (43.48%).