# Rapid Molecular Identification of Scolytinae (_Coleopterara: Curculionidae_)
## 2 Results
### Primer Pairs for Molecular Identification of Diverse Genera
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & \multicolumn{4}{c}{**ITS2 (ITS2F/ITS2R)**} \\ \hline \multirow{2}{*}{**Sample**} & \multirow{2}{*}{**Genus**} & **PCR** & \multirow{2}{*}{**GenBank Match**} & **Accession** & \multirow{2}{*}{**\% Identical**} \\  & & **Concentration** & & & **(ng/\(\mu\)L)** & \\ \hline B2 & _Coptoborus pseudotemuis_ & 12.70 & No match & & \\ B4 & _C. pseudotemuis_ & & No amplification & & \\ B6 & _C. pseudotemuis_ & & No amplification & & \\ B7a & _C. pseudotemuis_ & 25.80 & No match & & \\ B7b & _C. pseudotemuis_ & 12.10 & _Pitygens chalcographus_ & JQ066311 & 100.0\% \\  & & & _Galapapanus_ spp. & EU74896 & 100.0\% \\ \hline B3 & _Xyleborus ferrugineus_ & 21.00 & _Pitygens chalcographus_ & JQ066311 & 100.0\% \\  & & & _Galapreturns_ spp. & EU748796 & 100.0\% \\ \hline B14 & _Theoborus_ sp. & No amplification & & \\ BC1 & _Theoborus_ sp. & No amplification & & \\ BC9 & _Theoborus_ sp. & No amplification & & \\ BC13 & _Theoborus_ sp. & No amplification & & \\ \hline B16 & _Hypothenemus_ sp. & No amplification & & \\ B17 & _Hypothenemus_ sp. & No amplification & & \\ B19 & _Hypothenemus_ sp. & No amplification & & \\ B20 & _Hypothenemus_ sp. & No amplification & & \\ \hline J1 & _Arnplus_ sp. & No amplification & & \\ J2 & _Arnplus_ sp. & No amplification & & \\ J4 & _Arnplus_ sp. & No amplification & & \\ \hline \hline \end{tabular}
\end{table} 
Table 6: BLASTn results for ITS2 sequences amplified and sequenced with primers ITS2F and ITS2R. Concentration of PCR product sent for sequencing also shown to the left of the GenBank match. No match was determined by a query coverage \(>\) 30%. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & \multicolumn{4}{c}{**COI (LCO1490/HCO2198)**} \\ \hline \multirow{2}{*}{**Sample**} & \multirow{2}{*}{**Genus**} & **PCR** & \multirow{2}{*}{**GenBank Match**} & **Accession** & \multirow{2}{*}{**\% Identical**} \\  & & **(ng/\(\mu\)L)** & & & \\ \hline B2 & _Coptoborus pseudotemuis_ & 23.2 & Poor sequence & & \\ B4 & _C. pseudotemuis_ & 12 & Poor sequence & & \\ B6 & _C. pseudotemuis_ & 7.75 & Poor sequence & & \\ B7a & _C. pseudotemuis_ & 12.7 & _Coptodyras_ sp. & HM064072 & 86.4\% \\  & & & & C. _pseudotemuis_ & HM064071 & 85.5\% \\  & & & & C. _pseudotemuis_ & HM064072 & 84.7\% \\  & & & & C. _pseudotemuis_ & HM064071 & 84.3\% \\ \hline B3 & _Xyleborus ferrugineus_ & 60 & _Xyleborus ferrugineus_ & KP941251 & 98.0\% \\ \hline B14 & _Theoborus_ sp. & & No amplification & & \\ BC1 & _Theoborus_ sp. & & No amplification & & \\ BC9 & _Theoborus_ sp. & 5.87 & _Theoborus_ sp. & HM064100 & 94.7\% \\ BC13 & _Theoborus_ sp. & 38.9 & _Theoborus_ sp. & HM064100 & 91.2\% \\ \hline B16 & _Hypothenemus_ sp. & 51 & Poor sequence & & \\ B17 & _Hypothenemus_ sp. & 6.26 & Poor sequence & & \\ B19 & _Hypothenemus_ sp. & 38.5 & Poor sequence & & \\ B20 & _Hypothenemus_ sp. & 21.3 & _Hypothenemus_ sp. & MK768187 & 97.8\% \\  & & & & KX035186 & 97.8\% \\ \hline J1 & _Arnplus_ sp. & 6.45 & Poor sequence & & \\ J2 & _Arnplus_ sp. & 8.38 & _Arnts carinifrons_ & MK768387 & 84.1\% \\ J4 & _Arnplus_ sp. & 7.25 & _Arnplus carinifrons_ & MK767301 & 84.4\% \\ \hline \hline \end{tabular}
\end{table} 
Table 7: BLASTn results for COI sequences amplified and sequenced with primers LCO1490 and HCO2198. Concentration of PCR product sent for sequencing also shown to the left of the GenBank match.