## 3 Results  
Lineal Growth, Oxidative and Cellulolytic Activities During Cultivation on Solid Agar Media Containing Various Types of Sawdust  
The WRF _C. laeye_ LE-BIN 1700 showed the same linear growth rate (on average, about 9.0 mm/day) on MEA medium supplemented with birch sawdust (MEA-B) and alder sawdust (MEA-A). The fungal growth rate on MEA supplemented with pine sawdust (MEA-P) was 1.5 times lower (approximately 6.0 mm/day) (Figure 1A). Enzyme activity testing using the application method showed that both total oxidative and carboxy methyl cellulase (CMCase) activities were induced on media containing all the sawdust types (Figure 1A,B). At the same time, the activity values measured on the sawdust-containing media did not differ significantly between the different wood species (\(p>0.05\)).  
Lineal Growth and Oxidative Activity During Cultivation on Solid Agar Media Containing Different Lignocellulosic Components  
_C. laeye_ LE-BIN 1700 demonstrated growth efficiency on an agar medium containing model lignocellulosic components (i.e., lignin, CMC, xylan, starch and pectin). In addition, lignocellulosic components added to the agar significantly (\(p<0.05\)) increased the fungal growth rate as compared with the control agar medium (Figure 2). Fungal mycelium showed similar growth rates (about 10.0 mm/day) on media containing lignin, xylans and CMC, but was slightly higher (about 12-13 mm/day) on media containing starch and pectin.  
Characterization of Enzymatic Activities, Mycelial Biomass and Acidity During Semi-Solid Cultivation on Media Containing Various Types of Savdust  
During the semi-solid cultivation of _C. laeev_ 1700 on the control GP medium and on the GP media containing birch, alder and pine sawdust (GP-B, GP-A and GP-P), oxidase, cellulose and hemicellulose enzymatic activities were measured in the culture liquid (Figure 3A). At the end of the 30-day cultivation, the pH value was measured and the amount of fungal biomass was determined (Figure 3B).  
During the cultivation of _C. laeev_ 1700, cellulase and hemicellulose activities were not observed on the substrates (Azo-CMC, Azo-Xylan, Azo-Xyloglucan and Azo-Arabinosylan). However, oxidase activity measured on the ABTS substrate was detected on all the media (Figure 3A). _C. laeev_ cultivation showed the lowest oxidative activity on the GP medium; the highest activity (28 U/mL) was reported on the 10 th day of the fungal culture growth. Oxidase activity was significantly induced in the sawdust-containing media. On the 10 th and 25 th day of the fungal culture growth, maxima of oxidase activity (84 and 114 U/mL, respectively) were observed on GP-A and GP-B media (Figure 3A). On GP-P medium with pine sawdust, the activity gradually increased and reached a value of about 170 U/mL on the 30 th day of cultivation.  
Compared to the GP medium, the growth of the fungal biomass increased significantly (\(p<0.05\)) on the sawdust-containing media; the maximum value was recorded on the GP-B medium with birch sawdust (Figure 3B). During its growth on the all media, _C. laeev_ slightly  
Figure 1: Growth rate (**A**), oxidative (**B**) and cellulolytic (**C**) activity during cultivation of _Crucibulum laeev_ LE-BIN 1700 on MEA media containing various types of wood sawdust—birch (MEA-B), alder (MEA-A) and pine (MEA-P). The MEA medium without a sawdust addition was used as a control. Different letters indicate statistical differences between culture media at the same time point (\(p<0.05\)).  
Figure 2: _Crucibulum laeev_ LE-BIN 1700 growth rate on control medium (only bacteriological Agar) and medium supplemented with different lignocellulosic compounds—lignin; birch xylan (Xylan B); larch xylan (Xylan L); carboxymethyl cellulose (CMC); starch and pectin. The same letters indicate that the values are not significantly different (\(p>0.05\)).  
acidified the culture liquids. In particular, the pH values were on average 4.8 \(\pm\) 0.2 units compared to 5.4 \(\pm\) 0.3 units measured at the beginning of the cultivation.