Previous reports have shown that saponins are the main terpenoids of medicinal value of _G. sinensis_ pods, and, thus far, there are more than 30 known saponins [3]. Moreover, the main bioactive substance occurring in a large proportion and with essential anti-tumor and anti-inflammatory roles in _G. sinensis_ pods is gledtisioside, which is a kind of triterpen saponin [4; 5; 6; 7]. Triterpenoid saponin consists of one pentacyclic sapogenin and several carbohydrate chains, and its main resource is isopentenyl diphosphate (IPP) produced from the mean-onate pathway (MVA) or the methylerythitol phosphate pathway (MEP) [8]. IPP, with its isomeric dimethylallyl diphosphate (DMAPP), is synthesized into geranyl diphosphate (GPP). Afterwards, IPP with GPP is further synthesized into farnesyl diphosphate (FPP). Then 2,3-oxidosqualene from two FPPs oxidized in sesquiterpenoid and triterpenoid biosynthesis (STTB) is cyclized for the pentacyclic skeleton by different oxidosqualene cyclases (OSCs) [9]. The pentacyclic skeleton is finally decorated with hydroxy by cytochrome P450 monooxygenenses (P450s) and carbohydrate chains catalyzed by UDP-dependent glycosyltransferase (UGTs) to form triterpen saponins [10]. Moreover, 2,3-oxidosqualene is not only the precursor of saponin, but also the precursor of sterols from steroid biosynthesis (STB) and its derivative in brassinosterol biosynthesis (BRB) [11]. Moreover, many intermediates during triterpen saponin biosynthesis are also important precursors of other terpenoids. For example, DMAPP and GPP are the only resource of cytokins in the zeatin pathway (ZTB) [12] and monoterpenoids in monoterenoid biosynthesis (MTB), respectively. However, unlike all primary metabolites, some terpenoids become necessary and beneficial for plant growth only when they reach a certain concentration, which is regulated by unique mechanisms in plants [13]. Among these mechanisms, regulation mediated by microRNA (miRNA) is especially important. Recently, several miRNAs involved in the STTB pathway have been mapped and validated in _X. strumarium_ L. using NGS technology. For example, mRNAs encoding the upstream enzymes in the pathways of terpenoid biosynthesis, including IPP and DMAPP, were predicted to be targeted by _miR7539_, _miR5021_, and _miR134_[14]. Moreover, a review further summarized the critical roles of the miRNA-mRNA module in terpenoid biosynthesis and accumulation, which opens a new perspective for further investigations [15]. The miRNA is a kind of single-strand, small non-coding RNA with a length of 21-24 nt and can regulate the development and stress responses of plants, such as disease resistance [16], flower blooming regulation [17], and the promotion of pod ripening [18; 19], by preventing mRNA translation or mRNA cleavage at the post-transcription level. Interestingly, previous investigations have shown that miRNAs participate in the secondary metabolite synthesis pathway, and the contents of some metabolites are co-regulated by multiple miRNAs in plants. For example, in _Arabidopsis thaliana_ ([PERSON], the expression of _MYBBL2_ was suppressed by both _miR858a_ and _HY5_ (elongated hypocotyl 5), which leads to activation of anthocyanin synthesis pathways [20]. In addition, _miR89662_, _miR894_, _miR172_, and _miR166_ were suggested to regulate saponin biosynthesis in _Chlorophyll borivilianum_ [PERSON]. [21], which indicated that miRNAs are key molecular determinants in the saponin biosynthesis pathway. However, thus far, there are few reports of miRNAs in _G. sinensis_, especially miRNAs involved in the regulation of gledtisioside synthesis, which varies with the development in _G. sinensis_ pods. Consequently, it is indispensable to investigate the involvement of miRNAs in triterpen saponin synthesis in _G. sinensis_. In this investigation, the conserved and special miRNAs were first identified among three different stages of pods (May, July, and September).