## 4 Discussion  
The flower and bract (inflorescence) of _Musa x paradisiaca_ are consumed as Thai medicinal plants, and they are recommended in foods for breastfeeding mothers. Thus, a beverage containing the aqueous extract of banana inflorescence was developed in this study. This beverage provides convenience for working mothers and is intended to increase milk lactation. [PERSON] et al. [7] reported that active compounds, such as saponins and tannins, in the Musa flower's aqueous extract demonstrated significant galactagogue properties in rats. [PERSON] et al. [13] reported that banana flowers contained tannins, saponins, reducing and non-reducing sugars, sterols, and triterpen. Banana bracts were found to contain anthocyanins, including delphinidin, pelargonidin, peonidin, and malvidin, which exhibited potent antioxidant and pharmacological activity. In this study, we extracted banana inflorescence, including the flowers and bracts, to investigate the bioactive compounds. The banana inflorescence extract contained \(\beta\)-sitosterol, flavonoids, saponin, and other phenolic compounds in accordance with the above reports. The main phenolic components in banana inflorescence were catechin and isoquercetin. \begin{table}
\begin{tabular}{l l l} \hline \hline
**Nutrition Facts** & \multicolumn{2}{c}{**Percent Thai RDI \({}^{*}\)**} \\ \hline Serving size: 1 bottle (350 mL) & Total carbohydrate 36 \(\pm\) 0.4 g & 12\% \\ \hline Amount per serving & Sugar 36 \(\pm\) 0.4 g & \\ \hline Total energy 140 \(\pm\) 4.0 kcal & Sodium 70 \(\pm\) 5.3 mg & 3\% \\ \hline Total fat 0 g & \multicolumn{2}{l}{* Percentage of nutrients for the Thai recommend daily intakes} \\ \hline Protein 0 g & \multicolumn{2}{l}{(Thai RDI) for the age of 6 years and up are based on the energy demand of 2000 kcal/day} \\ \hline \hline \end{tabular} Values are expressed as means \(\pm\) standard deviation. * refers to Percentage of nutrients for the Thai recommend daily intakes (Thai RDI) for the age of 6 years and up are based on the energy demand of 2000 kcal/day.  
\end{table} 
Table 4: The nutrition facts for the beverage containing the aqueous extracts of banana inflorescence. \begin{table}
\begin{tabular}{l c c c} \hline \hline
**Pathogenic Microbes** & **Results** & **Unit** & **Reference** \\ \hline _Bacillus cereus_ & \textless{}1 & CFU/mL & ISO 7932: 2004 \\  & & & FDA BAM (Food and Drug Administration’s \\ _Clostridium perfringens_ & \textless{}1 & CFU/mL & Bacteriological Analytical Manual) Online, 2001 \\  & & & (Chapter 16) \\  & & & Standard Methods for the Examination of Water \\ Coliform & \textless{}1.1 & MPN/100 mL & and Wastewater APHA, AWWA, WEF, 23 rd \\  & & & Edition, 2017, Part 9221 \\ _Escherichia coli_ & Not Detected & In 100 mL & and Wastewater APHA, AWWA, WEF, 23 rd \\  & & & Edition, 2017, Part 9221 \\ _Salmonella_ spp. & Not Detected & In 25 mL & ISO 6579-1: 2017 (E) \\ _Staphylococcus aureus_ & Not Detected & In 0.1 mL & FDA BAM Online, 2016 (Chapter 12) \\ Yeast and Molds & \textless{}1 & CFU/mL & FDA BAM Online, 2001 (Chapter 18) \\ \hline \hline \end{tabular}
\end{table} 
Table 5: Microbial analysis of the banana inflorescence beverage. Polyphenols are one of the largest groups of phytochemicals, and they contain a class of flavonoids. The flavonoids consist of flavones, isoflavones, flavanones, catechins, and anthocyanins. The banana inflorescences are rich in anthocyanins [14] and demonstrated a high total presence of phenolic compounds, especially flavonoid, catechin, and isoquercetin in this study. Regarding the research of [PERSON], whether flavonoids caused an increase in milk lactation in mothers who were breastfeeding still remains unclear; however, they were demonstrated to protect mothers from oxidative damage, which leads to chronic diseases. The consumption of fruits and vegetables is recommended during both pregnancy and lactation [15]. The efficacy of the antioxidant properties of banana inflorescences was found in polar rather than non-polar solvents.