## 3 Results and Discussion
### Quantification of the Polyphenols with UPLC-Q-TOF-MS
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & **Compound** & **Rt** & **\(\lambda_{\text{max}}\)** & **[M-H] _m/z_** & **Concentration** \\ \hline \multicolumn{6}{c}{HYDROIXSBALE TANNINS} \\ \hline \multicolumn{6}{c}{Ellagitannis} \\ \hline
**2** & Galloyl-bis-HHDP-glucose I & 2.78 & 276 & 935 & 637, 467 & 138.28 \(\pm\) 9.98 \\
**3** & Galloyl-chebuloyl-HHDP-glucose acid & 2.88 & 276 & 953 & 785, 301 & 77.41 \(\pm\) 7.04 \\
**4** & Galloyl-bis-HHDP-glucose II & 2.92 & 276 & 935 & 637, 467 & 132.47 \(\pm\) 7.08 \\
**5** & Galloyl-bis-HHDP-glucose III & 2.99 & 276 & 935 & 767, 467 & 59.29 \(\pm\) 2.40 \\
**10** & Galloyl-bis-HHDP-glucose IV & 3.73 & 276 & 935 & 637, 467 & 29.48 \(\pm\) 1.12 \\ \hline \multicolumn{6}{c}{Gallotannis} \\ \hline
**9** & Trigalloyl-glucose & 3.68 & 269 & 635 & 465 & 13.51 \(\pm\) 0.19 \\ \hline \multicolumn{6}{c}{FLAVONIDS} \\ \hline
**14** & Quercetin 3-\(O\)-glucuronide & 4.65 & \(255,353\) & 477 & 301 & 137.73 \(\pm\) 4.19 \\
**15** & Quercetin 3-\(O\)-glucose & 4.74 & \(255,353\) & 463 & 301 & 184.30 \(\pm\) 4.02 \\
**17** & Kaempferol 3-\(O\)-glucose & 5.30 & \(264,347\) & 447 & 285 & 61.60 \(\pm\) 1.39 \\
**18** & Isorhamentin 3-\(O\)-glucose & 5.49 & \(253,352\) & 477 & 315 & 22.33 \(\pm\) 0.64 \\ \hline \multicolumn{6}{c}{PHENIXOL GLUCOSIDE DERIVATIVES} \\ \hline
**6** & Digalloyl phenol glucoside (chesnatin) & 3.17 & 272 & 637 & 467, 305 & 90.38 \(\pm\) 2.68 \\
**8** & Digalloyl phenol glucoside (isochsenatin) & 3.59 & 271 & 637 & 467 & 42.18 \(\pm\) 1.43 \\
**11** & Galloyl phenol glucoside (cretanin) & 4.01 & 274 & 469 & 169 & 15.04 \(\pm\) 1.16 \\
**13** & Galloyl phenol glucoside dimer (chestanin) & 4.37 & 274 & 937 & 467, 301 & 266.81 \(\pm\) 8.36 \\
**16** & Galloyl phenol glucoside dimer (isochsenatin) & 5.04 & 269 & 937 & 637, 467 & 39.54 \(\pm\) 1.29 \\ \hline \multicolumn{6}{c}{PHENOLIC ACID DERIVATIVES} \\ \hline
**1** & Gallic acid & 2.35 & 270 & 169 & 125 & 81.52 \(\pm\) 0.99 \\
**7** & Methyl-gallate & 3.53 & 269 & 183 & 168 & 19.07 \(\pm\) 0.17 \\
**12** & Elagic acid pentoside & 4.12 & 274 & 433 & 301 & 15.59 \(\pm\) 2.49 \\ \hline \multicolumn{6}{c}{TOTAL} \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Individual phenolic compounds identified by UPLC-PDA-MS/MS in _C. sativa_ leaf extract. nostic mass ion of \(m/\)/\(\gtrsim\) 183 and its mass fragments [M-H-15]- of \(m/\)/z 168 after the loss of the methyl group. Instead, ellagic acid pentoside (**12**) showed [M-H]- at \(m/\)z 433 and a fragmentation ion [M-H-132]- at 301 arising from the loss of the pentosyl unit. All of the compounds were previously characterized in \(C\). _sativa_ leaf extracts [10, 11, 36, 37, 38]. The total amount of flavonoids of \(C\). _sativa_ leaves was 1426.55 \(\mathrm{\SIUnitSymbolMicro g}/10\) mg of dried plant material. Chestanin was the most abundant component with 18.70%, followed by quercetin 3-O-glucose and galloyl-bis-HHDP-glucose isomer I with 12.92% and 9.69%, respectively. Our results are in agreement with previous reports on the chestnut leaves, where the major constituents were ellagitains, phenol glucoside and quercetin derivatives [36, 37].