# Complete Genome Report of a Hydrocarbon-Degrading _Sphingobium yanoikuyae_ S72
## 5 Conclusions  
_S. yanoikuyae S72_ was isolated from northern Mexico. S72 used selected hydrocarbons as unique carbon source for its growth. GC-MS analysis showed that S72 effectively degraded biphenyl, phenanthrene, and naphthalene at a concentration \(\leq\)100 \(\upmu\)g/mL. It also showed some activities on xylose and toluene but did not achieve a greater percentage of degradation activities in them. Genome analysis revealed some genes that are responsible for the degradation of the tested hydrocarbon. Most of these genes are involved in the peripheral pathway for the degradation of the tested hydrocarbon. Although some genes that are associated with the degradation of hydrocarbon were not found in S72, there were many hypothetical genes in S72 in the same region as the genes that are involved in the degradation of hydrocarbons. These hypothetical genes may have roles in the degradation of the tested hydrocarbon as observed in the experimental study; thus, a further transcriptomic study may help to find out the functions of these genes in S72's ability to degrade hydrocarbons. _S. yunokiuque_ S72 strain has the potential to be used as an agent for cleaning up environments contaminated with petroleum and other chemical pollutants. However, the use of microorganisms for bioremediation are highly dependent on other abiotic and biotic soil parameters, thus necessitating further in situ studies to investigate its activity and ability to survive in hydrocarbon-contaminated soils would be essential.  
The following supporting information can be downloaded at: [[https://www.mdpi.com/article/10.3390/app12126201/s1](https://www.mdpi.com/article/10.3390/app12126201/s1)]([https://www.mdpi.com/article/10.3390/app12126201/s1](https://www.mdpi.com/article/10.3390/app12126201/s1)), Figure S1: Morphological features. (A) Scanning electron micrographs of cells Sphingobium yanoikuyae strain S72. Scale bar, 500 nm. The presence of a single polar flagellum and a small globular formation is observed. (B) Transmission electron micrographs of S. yanoikuyae strain S72. Scale bars 0.5 \(\upmu\)m. Figure S2: Gas column chromatography image for the degradation of biphenyl by S72- The images showed the images for the culture medium without bacteria and biphenyl, culture with S72 and biphenyl and culture with biphenyl without S72; Figure S3: Gene region in Sphingobium yanoikuyae S72 that are associated with PAH degradation and showing hypothetical genes that may also be helping in the degradation of PAH; Table S1: COG comparison between S72 and other PAH degrading bacteria retrieved from the IMG database; Table S2: List of genes in Sphingobium yanoikuyae S72 which is associated with the degradation of S72.  
[PERSON] and [PERSON] designed and carried out the experimental study. [PERSON] wrote the manuscript. [PERSON] and [PERSON] supervised the study. [PERSON] carried out the genome assembly and analysis. [PERSON] edited the manuscript in English. All authors have read and agreed to the published version of the manuscript.  
There is no special funding for this study.  
Not applicable.  
The complete genome sequence of _S. yunokiuque_ S72 was deposited at GenBank with accession number CP023741. This strain is deposited at laboratory interaction environment-microorganism culture collection center.  
All authors want to acknowledge [PERSON], [PERSON], and [PERSON] for technical assistance and suggestion to improve the genome analysis.  
All authors declare that they do not have any conflict of interest.