## 3 Results and Discussion  
### Scanning Electron Microscopy Analysis  
Figure 1 presents scanning electron microscopy (SEM) images of the Fe\({}_{3}\)O\({}_{4}\)-Ag sample. These images provide insights into the morphology and elemental composition of the nanoparticles. Figure 1a displays an SE-HA (High-Angle Secondary Electron) image captured using high-angle secondary electron detectors. This image reveals the nanoparticle topology, clearly indicating the presence of Fe\({}_{3}\)O\({}_{4}\) and Ag phases. Figure 1b depicts an SE(U) image, corroborating the presence of Fe\({}_{3}\)O\({}_{4}\) and Ag. These images utilize secondary electron emission from the sample surface upon primary electron beam interaction, providing high-resolution surface topography details. Figure 1c showcases an HA(T) (High-Angle Backscattered Electron) image, obtained using high-angle backscattered electron detectors, highlighting the formation of Fe\({}_{3}\)O\({}_{4}\)-Ag. Lastly, Figure 1d illustrates an SE + HA + OFF image, indicating the simultaneous use of both secondary and backscattered electron detectors, with the secondary electron detector turned off. This combined imaging approach offers a comprehensive view of both surface morphology and elemental composition. All images were captured at \(\times\)500k magnification, with a working distance of 3.7 mm and an acceleration voltage of 3.0 kV. The SEM imaging results suggest the formation of a decorated Fe\({}_{3}\)O\({}_{4}\)-Ag structure. Figure 1e schematically describes the distribution of the Fe\({}_{3}\)O\({}_{4}\) and Ag nanostructures, whose distribution can be attributed to variations in the nucleation and growth rates of magnetite and silver structures. The successful synthesis of these decorated structures is evident from the captured images. This underscores the efficacy of the proposed methodology in fabricating such decorated structures.  
The chemical analysis depicted in Figure 2 was conducted using Energy Dispersive X-ray Spectroscopy (EDS). In Figure 2a, the secondary electron (SE) image of the Fe\({}_{3}\)O\({}_{4}\) configuration is presented. In Figure 2b, a mapping of the sample illustrates the distribution  
Figure 1: SEM micrographs depicting the Fe\({}_{3}\)O\({}_{4}\)-Ag nanoparticle sample. (**a**) SE-HA ima.e showing nanoparticle topology and the presence of Fe\({}_{3}\)O\({}_{4}\) and Ag phases. (**b**) SE(U) image confirming Fe\({}_{3}\)O\({}_{4}\) and Ag presence, providing high-resolution surface details. (**c**) HA(T) image emphasizing Fe\({}_{3}\)O\({}_{4}\)-Ag formation. (**d**) SE + HA + OFF composite image displaying surface morphology and elemental composition. Magnification: \(\times\)500k; Working distance: 3.7 mm; Acceleration voltage: 3.0 kV. (**e**) Scheme of the distribution of Fe\({}_{3}\)O\({}_{4}\) and Ag nanostructures corresponding to the decorated type Fe\({}_{3}\)O\({}_{4}\)-Ag nanoparticle samples.  
of silver (Ag) on the iron oxide structure, confirming the formation of the decorated Fe\({}_{3}\)O\({}_{4}\)-Ag structure. Subsequently, Figure 2c-e display elemental mappings of iron (Fe), oxygen (O), and silver (Ag) from the SE image of Fe\({}_{3}\)O\({}_{4}\). Lastly, Figure 2f presents a chemical analysis of the Fe\({}_{3}\)O\({}_{4}\) sample, confirming the predominant presence of elements such as Fe, O, and Ag. These results elucidate the chemical composition of the decorated nanoparticles, whose cytotoxic performance will be further investigated in this study.