## 4 Conclusions  
In the present study, a method of analysis for quality control of ISYPT was developed using a HPLC-PDA system. The method for simultaneous analysis of representative marker constituents was verified using various parameters, including linearity, LOD, LOQ, accuracy, and precision. This analytical method can be used to characterize the herbal medicine prescription for basic research. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Constituent**} & \multicolumn{4}{c}{**Concentration (mg/g)**} \\ \cline{2-5}  & \multicolumn{2}{c}{**DW Solution**} & \multicolumn{2}{c}{**70\% MeOH Solution**} \\ \cline{2-5}  & \multicolumn{2}{c}{**Mean \(\pm\) SD (\(\times\)10\({}^{-1}\))**} & \multicolumn{2}{c}{**RSD (\%)**} & \multicolumn{2}{c}{**Mean \(\pm\) SD (\(\times\)10\({}^{-1}\))**} & \multicolumn{2}{c}{**RSD (\%)**} \\ \hline MBRSA & 5.29 \(\pm\) 0.24 & 0.45 & 5.95 \(\pm\) 0.06 & 0.10 \\ LQRTA & 1.86 \(\pm\) 0.06 & 0.31 & 1.97 \(\pm\) 0.14 & 0.70 \\ LQRT & 0.34 \(\pm\) 0.03 & 0.80 & 0.34 \(\pm\) 0.02 & 0.65 \\ NRG & 3.06 \(\pm\) 0.10 & 0.33 & 3.33 \(\pm\) 0.29 & 0.87 \\ DDM & 0.60 \(\pm\) 0.03 & 0.58 & 0.66 \(\pm\) 0.01 & 0.14 \\ PCR & 4.23 \(\pm\) 0.14 & 0.32 & 4.84 \(\pm\) 0.04 & 0.08 \\ GCRZA & 1.51 \(\pm\) 0.01 & 0.09 & 1.80 \(\pm\) 0.05 & 0.27 \\ SZDRA & 0.09 \(\pm\) 0.01 & 1.20 & 0.11 \(\pm\) 0.01 & 1.27 \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Concentrations of eight representative marker constituents in ISYPT samples by HPLC-PDA (\(n=3\)). \begin{table}
\begin{tabular}{c c c c c c c c c c} \hline \hline \multirow{2}{*}{**Analyte**} & \multicolumn{4}{c}{**Intraday (\(n=5\))**} & \multicolumn{4}{c}{**Interday (\(n=5\))**} & \multicolumn{4}{c}{**Repeatability (\(n=6\))**} \\ \cline{2-9}  & \multirow{2}{*}{ \begin{tabular}{c} **Conc.** \\ **(\#g/mL)** \\ \end{tabular}  } & \multirow{2}{*}{ \begin{tabular}{c} **Found** \\ **Conc.** \\ **(\#g/mL)** \\ \end{tabular}  } & \multirow{2}{*}{ \begin{tabular}{c} **Precision** \\ **(\#g/mL)** \\ \end{tabular}  } & \multirow{2}{*}{ \begin{tabular}{c} **Accuracy** \\ **(\%)** \\ \end{tabular}  } & \multirow{2}{*}{ \begin{tabular}{c} **Found** \\ **(\#g/mL)** \\ \end{tabular}  } & \multirow{2}{*}{ \begin{tabular}{c} **Precision** \\ **(\#g/mL)** \\ \end{tabular}  } & \multirow{2}{*}{ \begin{tabular}{c} **Accuracy** \\ **(\%)** \\ \end{tabular}  } & \multirow{2}{*}{ \begin{tabular}{c} **RSD (\%)** \\ **(\%)** \\ \end{tabular}  } & \multirow{2}{*}{ \begin{tabular}{c} **RSD (\%)** \\ **(\%)** \\ \end{tabular}  } & \multirow{2}{*}{ \begin{tabular}{c} **RSD (\%)** \\ **(\%)** \\ \end{tabular}  } & \multirow{2}{*}{\ \begin{tabular}{c} **RSD (\%)** \\ **(\%)** \\ \end{tabular}  } & \multirow{2}{*}{ \begin{tabular}{c} **RSD (\%)** \\ **(\%)** \\ \end{tabular}  } \\ \hline MBRSA & 25.00 & 25.22 & 0.68 & 100.87 & 26.33 & 3.72 & 105.30 & & \\ MBRSA & 50.00 & 50.94 & 1.60 & 101.88 & 52.77 & 3.20 & 105.55 & 0.04 & 0.72 \\  & 100.00 & 105.58 & 1.75 & 105.58 & 107.74 & 2.10 & 107.74 & & \\ \hline LQRTA & 12.50 & 12.63 & 0.74 & 101.06 & 13.19 & 3.77 & 105.54 & & \\ LQRTA & 25.00 & 25.50 & 2.07 & 102.01 & 26.40  
**Supplementary Materials:** The following are available online at [[https://www.mdpi.com/article/10.3390/app11114819/s1](https://www.mdpi.com/article/10.3390/app11114819/s1)]([https://www.mdpi.com/article/10.3390/app11114819/s1](https://www.mdpi.com/article/10.3390/app11114819/s1)), Figure S1: Chemical structures of eight representative marker constituents of ISYPT; Figure S2: HPLC chromatograms of constituents of the herbal medicine formula identifying its major components. (**a**): _B. falciparum_; (**b**): _M. allba_; (**c**): _P. cocos_; (**d**): _S. chinensis_; (**e**): _F. thunbergii_; (**f**): _P. armeniaca_; (**g**): _P. trifoliate_; (**h**): _P. grandiflora_; (**i**): _P. ginseng_; (**j**): _E. asinus_; (**k**): _G. uralensis_; (**l**): _Z. offcimale_; and (**m**): _Z. juju_; Figure S3: HPLC chromatograms of the standard solution (**a**) DW extract (**b**), and 70% MeOH extract (**c**) of ISYPT samples.