## 3 Results
### Temperature Analysis  
Analysis of the temperature of the glass wall of the bottle in the water bath, from 0 to 15 min, showed that the temperature increased with the exposure time of the culture medium in the microwave oven, in an exponential manner (Figure 2). When the bottle was placed in the microwave oven for 15 min, a wall temperature higher than 130 \({}^{\circ}\)C was reached. After exposure for the duration that allows complete sterilization, set at 7 min according to the above results, approximately 90 \({}^{\circ}\)C was reached, which is lower than the autoclaving temperature (usually 121 \({}^{\circ}\)C for 15 min). Quality Control and Effect of Sterilization in a Microwave Oven vs. Autoclave on the Productivity of SDA  
In the analysis of productivity using the pour plate method, significant differences were observed depending on the exposure time in the microwave concerning _C. albicans_ and _S. cerevisiae_, while for _A. brasiliensis_ ATCC 16404, the productivity results were similar in all media, regardless of the exposure time in the microwave oven, with CFU counts similar to those obtained for the autoclave media (Figure 3). Figure 2: Registered temperature of the glass bottle wall over time, measured with an infrared thermometer, during microwave treatment at different times. For _C. albicans_ ATCC 10231, significant differences in the mean number of CFU were observed among the different durations assayed (_p_ < 0.001; GLM), specifically among the different microwave oven exposure times, as follows: 7 min vs. 10 min, 7 min vs. 13 min, and 7 min vs. 15 min (_p_ = 0.001; Tukey) (Figure 3). The highest productivity occurred in the media sterilized for 10 min (56.20 \(\pm\) 5.07 CFU), followed by 15 min (55.73 \(\pm\) 4.54 CFU), 13 min (55.13 \(\pm\) 2.52 CFU), and 7 min (40.80 \(\pm\) 7.68 CFU). No statistically significant differences in the productivity obtained in the medium sterilized in the autoclave (121 \({}^{\circ}\)C, 15 min) (47.4 \(\pm\) 1.14 CFU) were observed in comparison to the productivity obtained in the microwave oven at the different exposure times (Figure 3). In _S. cerevisiae_ ATCC 9763, a significantly higher CFU count was observed as the exposure time in the microwave increased, as follows: 7 min vs. 13 min (_p_ = 0.019; Tukey) and 7 min vs. 15 min (_p_ = 0.015; GLM) (Figure 3), with the highest productivity at 15 min (57.87 \(\pm\) 6.13 CFU), followed by 13 min (57.66 \(\pm\) 2.64 CFU), 10 min (55.93 \(\pm\) 9.02 CFU), and 7 min (49.87 \(\pm\) 6.77 CFU) (Figure 3). In the media sterilized through autoclaving, no significant differences were observed between the autoclaved media (59.40 \(\pm\) 10.23 CFU) and any of the media exposed to the microwave (Figure 3). Quality Control and Effect of Sterilization in a Microwave Oven vs. Autoclave on the Productivity of TSA  
For the TSA (Figure 4), the mean number of CFU were similar between the microwave exposure time and the use of the autoclave. As shown in Figure 4, for _E. coli_, _C. albicans_, and _S._ ser. Typhimurium, no statistically significant differences were observed in the productivity at the different exposure times in the microwave oven, and the productivity obtained in the autoclave was similar to that of the microwave oven, regardless of the exposure time. For _S. aureus_ ATCC 6538, similar growth was obtained with the different exposure times in the microwave oven, but a significantly lower number of CFU were obtained in the autoclave (67.80 \(\pm\) 7.73 CFU) vs. the microwave oven, as follows: 7 min (83.73 \(\pm\) 3.60 CFU; \(p\) = 0.005; [PERSON]), 10 min (86.87 \(\pm\) 9.32 CFU; \(p\) < 0.001; [PERSON]), 13 min (86.93 \(\pm\) 3.21 CFU; \(p\) < 0.001; [PERSON]), and 15 min (82.33 \(\pm\) 1.70 CFU; \(p\) = 0.013; [PERSON]). This trend was also observed for _B.