# Quantification of the Area of the Highest Temperature in
## 3 Results  
In the infrared images representing the entire body surface of the imaged animals, the horse group exhibited a pixel count ranging from 203,638 to 320,695 pixels, while the foal group ranged from 205,421 to 322,355 pixels. In the horse group, these pixels displayed from 15 to 21 colors, while in the foal group, 18 to 22 colors were displayed (Figure 4). The main colors detected in the infrared images are presented in Figures 5 and 6. In the horse group, pixels in shades of dark green (Figure 5A-D,F-H) and light green (Figure 5E,I-L) were most frequently detected. Pixels in shades of light blue (Figure 5A,B,E) and dark blue (Figure 5F,G) were less common. In the foal group, pixels in shades of green, both dark and light, were most prevalent (Figure 6A,B,E,F-L), whereas pixels in shades of blue were detected less frequently (Figure 6C,D). In both groups, pixels in shades of red, purple, and orange accounted for a smaller percentage. However, there was no significant difference in Total pixels between the horse and foal groups (\(p\) = 0.17; Figure 7A). On the other hand, Tmax was higher in the foal group than the horse group (\(p\) < 0.0001; Figure 7B). Tmax ranged from 29.8 \({}^{\circ}\)C to 31.4 \({}^{\circ}\)C in the horse group, whereas in the foal group, Tmax ranged from 31.5 \({}^{\circ}\)C to 33.6 \({}^{\circ}\)C. Interestingly, the highest temperature values were observed in horses in the eye, nostrils, and temporal regions, whereas in foals, these were only in the eye region (Table 1). The similarities between the Total pixels detected in the horse and foal groups throughout ten Areas of Tmax were tested using a linear regression model. The slope of the linear regression equations for Total pixels in the horse group compared to the slopes of Total pixels in the foal group was significantly different (\(p\) < 0.0001); thus, no single slope was calculated (Figure 5C). Therefore, no evidence of similarity in the distribution of Total pixels between these two groups can be noted. \begin{table}
\begin{tabular}{c c c c c c c c c c c c c} \hline \hline
Figure 5: Percentage of pixels belonging to individual HEX colors detected on the horses’ body surface. Subsequent horses were considered separately as individuals 1-12 **(A–L)**. Percentage of pixels < 1.5% is not displayed. Figure 6: The percentage of pixels belonging to individual HEX colors detected on the foals’ body surface. Subsequent foals were considered separately as individuals 1–12 (**A–L**). Percentage of pixels < 1.5% is not displayed. Then, the % of Color pixels was compared between the horse and foal groups for each Area of the Tmax separately. The % of Color pixels was found to be higher in the horse group than in the foal group in the following ranges: Tmax-10 \({}^{\circ}\)C (_p_ < 0.0001; Figure 8A), Tmax-9 \({}^{\circ}\)C (_p_ < 0.0001; Figure 8B), Tmax-8 \({}^{\circ}\)C (_p_ < 0.0001; Figure 8C), Tmax-7 \({}^{\circ}\)C (_p_ = 0.0001; Figure 8D), Tmax-6 \({}^{\circ}\)C (_p_ = 0.003; Figure 8E), Tmax-5 \({}^{\circ}\)C (_p_ = 0.0002; Figure 8F), Tmax-4 \({}^{\circ}\)C (_p_ < 0.0001; Figure 8G), Tmax-3 \({}^{\circ}\)C (_p_ < 0.0001; Figure 8H), Tmax-2 \({}^{\circ}\)C (_p_ = 0.0003; Figure 8I), and Tmax-1 \({}^{\circ}\)C (_p_ = 0.03; Figure 8J). Similarly, the % of Red pixels was compared between the horse and foal groups for each Area of the Tmax separately. The % of Red pixels was higher in the horse group than in the foal group in the following ranges: Tmax-10 \({}^{\circ}\)C (_p_ < 0.0001; Figure 9A), Tmax-9 \({}^{\circ}\)C (_p_ < 0.0001; Figure 9B), Tmax-8 \({}^{\circ}\)C (_p_ < 0.0001; Figure 9C), Tmax-7 \({}^{\circ}\)C (_p_ < 0.0001; Figure 9D), Tmax-6 \({}^{\circ}\)C (_p_ = 0.002; Figure 9E), Tmax-5 \({}^{\circ}\)C (_p_ = 0.0008; Figure 9F), Tmax-4 \({}^{\circ}\)C (_p_ < 0.0001; Figure 9G), Tmax-3 \({}^{\circ}\)C (_p_ < 0.0001; Figure 9H), Tmax-2 \({}^{\circ}\)C (_p_ = 0.0004; Figure 9I), and Tmax-1 \({}^{\circ}\)C (_p_ = 0.008; Figure 9J).