## 3 Results and Discussion
### Genomic Characteristics  
In total, 155,957 quality reads were assembled in 22 scaffolds consisting of 29,620,091 bp (30\(\times\) average coverage; circularization achieved for one scaffold (mitochondrial genome); a GC content of 47.72%; Scaffold N50 of 4,060,673; an average scaffold length of 1,346,367.77, and the largest scaffold being 8,180,881 bp) (Table 2). The Funannotate, Barnrap, and ARAGORN software's predicted the occurrence of 10156 coding genes, with forty-four rRNAs (two 185 rRNAs, three 285 rRNAs (one solely partial), two 5.85 rRNAs and thirty and seven 55 rRNAs) and one hundred and seventy-eight tRNAs. The Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness was estimated to be at 99.6% for _Eurotiales_ (\(n\) = 4191), with 4174 complete BUSCOs, 4167 complete and single-copy BUSCOs, 7 complete and duplicated BUSCOs, 5 fragmented BUSCOs and 12 missing BUSCOs (Figure 4). \begin{table}
\begin{tabular}{c c} \hline \hline
**Info** & **Value** \\ \hline Scaffold number & 22 \\ Total scaffold length & 29,620,091 \\ Average scaffold length & 1,346,367.77 \\ Scaffold N50 & 4,060,673 \\ Scaffold auN & 4,720,784.16 \\ Scaffold L50 & 3 \\ Largest scaffold & 8,180,881 \\ Smallest scaffold & 7668 \\ N’s number & 0 \\ Read mean quality & 14.1 \\ Read median quality & 17.2 \\ Initial number of reads & 155,957 \\ Read mean quality & 14.1 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Overall genome assembly metrics. Figure 4: Genome completeness as evaluated with BUSCO and the _Eurotiales_ database (orthoDB v.10). [PERSON] and colleagues [63] conducted the largest study focusing on _Penicillium_ genomic characteristics with the Oxford Nanopore(r) sequencing methodology to date (93 isolates). They found that the estimated genomic sizes and gene numbers ranged from 25.4 to 46.5 Mb and contained 9591-14,319 coding genes, with an array of contigs/ scaffolds being between 5 and 65. Thus, the results obtained during the course of this work can be considered to be within the typical average ranges for the genus (29.6 Mb, 10,156 coding genes and 22 scaffolds). Currently, more than 400 _Penicillium_ genomes are publicly available [64]; yet, to the best of our knowledge, no genomic data exist neither for this species and neither for sect. _Paradoxa_, ser. _Aramentosa_. The functional genomic analysis based on the conducted annotation (Supplementary Table S1; Figure 5) pointed out that the top five most representative domains for (1) biological processes were as follows: the regulation of cellular process, cellular process, the positive regulation of cellular process, response to stress and the positive regulation of transcription by RNA polymerase II; (2) the cellular components were as follows: cytoplasm, cytosol, nucleus, membrane and plasma membrane; and (3) the molecular functions were as follows: protein binding, binding, identical protein binding, metal ion binding and ATP binding. These findings underscore a significant representation of biological, cellular and molecular traits associated with the regulation of cellular processes and transmembrane transport, as well as protein, metals and ATP binding functions. Somewhat similar results were reported by [PERSON] and colleagues [65] for the genome of _Penicillium_ pancosinium_ MUM 23.27 (isolated from raw honey) and could be a result from their survival needs in unusual extreme environments. InterProScan predicted the presence of 4482 protein families, 2929 protein domains, 424 protein sites and 62 protein repeats.