# Scented Tartary Buckwheat Tea: Aroma Components and Antioxidant Activity
## 2 Results and Discussion
### Total Flavonoid Content (TFC) and Total Phenolic Content (TPC) in Tartary Buckwheat Tea (TBT)
#### 2.4.1 Total Flavonoid Content (TFC) in the Tea Infusion of Tartary Buckwheat Tea (TBT)  
Buckwheat, which is rich in flavonoids, is the main source of rutin in the human diet. As shown in Table 2, the TFC of the tea infusion was determined spectrophotometrically, and ranged from 3.33 \(\pm\) 0.27 mg/g to 12.03 \(\pm\) 0.87 mg/g with significant differences (\(p\) < 0.05). The TFC levels of the TBC infusions were highest in the FTI at 12.03 \(\pm\) 0.87 mg/g, while the STI value was 8.98 \(\pm\) 0.84 mg/g. Following two brewing cycles, the TFC of the TBH infusion decreased by 34.34%, while that of TBR displayed no significant change with values of 3.73 \(\pm\) 0.12 mg/g in the FTI and 3.58 \(\pm\) 0.26 mg/g in the STI. Furthermore, the flavonoids of TBR exhibited the highest thermal stability of all the samples, while the opposite was apparent in TBH. Figure 5: The total flavonoid content (TFC) and total phenolic content (TPC) in tartary buckwheat tea (TBT) (The same letter in the Figures signifies no significant difference, and the letters a–d and p–s indicates the difference of the total flavonoid content (TFC) and total phenolic content (TPC), respectively (\(p\) < 0.05)). \begin{table}
\begin{tabular}{c c c c c c c c c c c} \hline \hline \multirow{2}{*}{**Sample**} & \multicolumn{2}{c}{**TPC (mg/g)**} & \multicolumn{2}{c}{**TPC (mg/g)**} & \multicolumn{2}{c}{**DPPH Free Radical Scavenging**} & \multicolumn{2}{c}{**\(\mathbf{\cdot\cdot\cdot\cdot}\)OH Radical Scavenging**} & \multicolumn{2}{c}{**Total Reducing Power/**} \\  & \multicolumn{2}{c}{**Ability/\%**} & \multicolumn{2}{c}{**Ability/\%**} & \multicolumn{2}{c}{**Ability/\%**} & \multicolumn{2}{c}{**Ability/\%**} & \multicolumn{2}{c}{**Ability/\%**} & \multicolumn{2}{c}{**(OD Values)**} \\ \cline{2-11}  & **FTI** & **STI** & **FTI** & **STI** & **FTI** & **STI** & **FTI** & **STI** & **STI** & **STI** & **STI** \\ \hline TBI & \(7.04\pm 0.10\) & \(4.62\pm 0.08\) & \(5.17\pm 0.06\) & \(4.14\pm 0.01\) & \(44.94\pm 1.63\) & \(39.06\pm 2.18\) & \(43.46\pm 4.32\) & \(27.78\pm 0.39\) & \(1.81\pm 0.01\) & \(1.32\pm 0.04\) \({}^{b}\) \\ TBC & \(12.03\pm 0.87\) & \(8.98\pm 0.84\) & \(7.84\pm 0.05\) & \(6.47\pm 0.19\) & \({}^{b}\) & \(71.76\pm 3.99\) & \(69.15\pm 5.04\) & \(85.90\pm 45.8\) & \(58.11\pm 1.61\) & \({}^{b}\) \\ TBR & \(3.73\pm 0.12\) & \(3.58\pm 0.26\) & \(9.49\pm 0.01\) & \(9.27\pm 0.09\) & \(68.04\pm 1.06\) & \(59.24\pm 2.05\) & \(97.24\pm 0.30\) & \(87.72\pm 0.92\) & \(2.28\pm 0.03\) & \(2.16\pm 0.11\) \({}^{a}\) \\ TBR & \(3.92\pm 0.10\) & \(3.33\pm 0.27\) & \(5.43\pm 0.01\) & \(4.85\pm 0.02\) & \(76.30\pm 1.19\) & \(62.33\pm 5.71\) & \(92.65\pm 1.85\) & \(98.46\pm 1.94\) & \(1.94\pm 0.05\) & \(1.58\pm 0.20\) \({}^{b}\) \\ \hline \end{tabular}  
* The same letter in the Figures signifies no significant difference, and the letters \(p>4\) and \(d\) indicates the difference of the first ten infusion (FTI) and second ten infusion (STI), respectively (\(p<0.05\)) (Abbreviations: TTH\(-\)Hunatal ternary backwheat two, TBC\(-\)tartary backwheat two, TIB\(-\)tartary backwheat two, TIB\(-\)tartary backwheat (same tea).  
\end{table} 
Table 2: Total flavonoid content (TPC), total phenolic content (TPC) and antioxidant ability in the tea infusion of tartary backwheat tea (TBT). Flavonoids are the most common and widely distributed phenolic compounds in plants [29]. [PERSON] et al. [9] reported that the flavonoid content dissolved in hot water displayed substantially lower levels than that of the TBT alcohol extracts, and the TFC determined in the tea infusion exhibited a 60% decline compared with that of the TBH alcohol extracts. Moreover, the TFC in the FTI of whole grain tea was \(4.66\pm 0.03\) mg/g, and whole plant tea was \(0.58\pm 0.01\) mg/g, which was \(7.04\pm 0.01\) mg/g in this study. Early research suggested that tartary buckwheat seeds contained rutin in the range of 800 mg to 1700 mg rutin/100 g DW, with only trace amounts of quercetin, the aglycone of rutin [30].