## 2 Results and Discussion
### Effect of Germination on the Volatile Profile of IWG
#### 2.2.3 MHE Optimization
Therefore, the logarithm of the peak areas does not display linearity with the number of extractions. Twenty-nine percent of the identified compounds were confirmed using authentic standards (Table 4). \begin{table}
\begin{tabular}{c c c c c c c c} \hline
**RT** & **Compounds** & **Odor Descriptors A** & **RI** & **RI Tab** & \(\Delta\) & **Identification** & \(\beta\) \\ \hline \multicolumn{8}{c}{Alcohols} \\ \hline
3.11 & 3-methyl-1-butanol & Malty & 776 & 735 & 24 & RL, MS, STD & 0.78 \\
3.6 & 1-pentanol & Fruity, ethereal & 795 & 780 & 15 & RL, MS & 0.78 \\
4.08 & 2,3-butanediol & Butter-like & 813 & 819 & \(-6\) & RL, MS & 0.78 \\
5.86 & 1-hexanol & Grassy & 883 & 880 & 3 & RL, MS & 0.78 \\
6.73 & 2-heptanol & Coconut-like & 916 & 900 & 16 & RL, MS, STD & 0.90 \\
7.94 & 1-butoxy-2-propanol & Ether-like B & 953 & 947 & 6 & RL, MS & 0.78 \\
8.96 & 1-heptanol & Fruity, soapy & 984 & 975 & 9 & RL, MS, STD & 0.78 \\
9.3 & 1-octen-3-ol & Mushroom-like & 994 & 986 & 8 & RL, MS, STD & 0.72 \\
11.15 & Benzyl alcohol & Bitter almond-like & 1047 & 1042 & 5 & RL, MS & 0.78 \\
12.37 & 2-octen-1-ol & Soapy & 1081 & 1067 & 14 & RL, MS & 0.51 \\
12.51 & 1-octanol & Soapy & 1085 & 1076 & 9 & RL, MS & 0.78 \\
13.94 & Phenylethyl alcohol & Floral & 1124 & 1121 & 3 & RL, MS & 0.78 \\
16.34 & p-menthan-3-ol & Peppermint-like & 1191 & 1170 & 21 & RL, MS & 0.78 \\ \hline \multicolumn{8}{c}{Aldehydes} \\ \hline
4.22 & Hexanal & Grassy & 819 & 817 & 2 & RL, MS, STD & 0.89 \\
5.44 & 2-hexanal & Green apple-like & 866 & 854 & 12 & RL, MS & 0.89 \\
6.79 & Heptanal & Fatty & 918 & 907 & 11 & RL, MS & 0.89 \\
8.51 & 2-heptenal & Green apple-like & 970 & 978 & \(-8\) & RL, MS, STD & 0.51 \\
8.65 & Benzaldehyde & Almond-like & 974 & 965 & 9 & RL, MS, STD & 0.51 \\
10.11 & Octanan & Green & 1018 & 1007 & 11 & RL, MS, STD & 0.89 \\
11.49 & Phenylacetaldehyde & Floral & 1056 & 1053 & 3 & RL, MS & 0.89 \\
12.04 & 2-octanal & Fatty & 1072 & 1061 & 11 & RL, MS, STD & 0.51 \\
13.74 & Nonanal & Soapy & 1119 & 1108 & 11 & RL, MS, STD & 0.89 \\
15.7 & 2-nonenal & Fatty & 1173 & 1171 & 2 & RL, MS & 0.51 \\ \hline \multicolumn{8}{c}{Ketones} \\ \hline
5.11 & 4-hydroxy-4-methyl-2-pentanone & Mint-like B & 854 & 850 & 4 & RL, MS, STD & 0.54 \\
5.5 & 5-methyl-2-hexanone & Ethereal & 869 & 857 & 12 & RL, MS & 0.84 \\
6.41 & 2-heptanone & Fruity & 904 & 898 & 6 & RL, MS, STD & 0.54 \\
6.98 & \(\gamma\)-buttyrolactone & Sweety & 923 & 922 & 1 & RL, MS & 0.54 \\
11.3 & 3-octen-2-one & Floral, spicy & 1051 & 1046 & 5 & RL, MS & 0.54 \\
11.73 & \(\gamma\)-caprolactone & Fruity & 1063 & 1055 & 8 & RL, MS, STD & 0.54 \\
12.25 & Acetophenone & Foxy & 1077 & 1073 & 4 & RL, MS, STD & 0.54 \\
12.42 & 3,5-octadien-2-one & Fatty, fruity & 1082 & 1093 & 11 & RL, MS & 0.54 \\
22.51 & \(\gamma\)-nonalactone & Coconut-like & 1369 & 1368 & 1 & RL, MS & 0.54 \\ \hline \multicolumn{8}{c}{Acids} \\ \hline
5.28 & 3-methyl-butanoic acid & Sweaty & 860 & 834 & 26 & RL, MS & 0.78 \\
6.24 & Pentanoic acid & Sweaty, fruity & 897 & 875 & 22 & RL, MS & 0.90 \\
22.94 & Decanoic acid & Soapy, musty & 1382 & 1380 & 2 & RL, MS & 0.90 \\
39.51 & Hexadecanoic acid & Waxy B & 1967 & 1960 & 7 & RL, MS & 0.90 \\ \hline \multicolumn{8}{c}{Esters} \\ \hline
9.86 & Butyl lbutanoate & Sweet, fruity & 1011 & 993 & 18 & RL, MS & 0.64 \\
20.04 & Bornyl acetate & Pine-like & 1296 & 1291 & 5 & RL, MS & 0.64 \\
40.37 & Ethyl palmitate & Waxy B & 2001 & 1996 & 5 & RL, MS & 0.90 \\
44.18 & Ethyl cis,cis-9,12-octadecadiene & - & 2166 & 2159 & 7 & RL, MS & 0.90 \\
44.34 & (E)-9-octadecenoic acid ethyl ester & - & 2173 & 2174 & \(-1\) & RL, MS & 0.90 \\ \hline \end{tabular}
\end{table} 
Table 4: Volatile compounds identified by HS-SPME and GC-MS in IWG samples under optimal extraction conditions. Nine representative compounds from different chemical classes were used to create calibration curves and perform the quantification. The method was validated, and the validation parameters are presented in Table 5. The method demonstrated adequate linearity, with R\({}^{2}\) values higher than 0.99.