## 3 Results and Discussion
### Celluluse Assay  
Maximum total (Table 2), exoglucanase (Table 3), endoglucanase (Table 4) and \(\beta\)-glucosidase (Table 5) activities developed after 6 weeks of cultivation. At the same time, pH 8.5 of the medium was the most optimal for the synthesis of cellulase enzymes. The maximum total cellulase activity was 0.326 U/mL\({}^{-1}\), exoglucanase--0.539 U/mL\({}^{-1}\), endoglucanase--0.950 U/mL\({}^{-1}\) and \(\beta\)-glucosidase was 0.795 U/mL\({}^{-1}\). \begin{table}
\begin{tabular}{c c c c} \hline \multirow{2}{*}{**Cultivation Period,**} & \multicolumn{3}{c}{**Activity (U mL\({}^{-1}\)) \(\pm\) SD**} \\  & \multicolumn{3}{c}{**pH**} \\ \cline{2-4}  & **5.5** & **7.0** & **8.5** \\ \hline
1 & 0.031 \(\pm\) 0.002 aa & 0.047 \(\pm\) 0.002 aa & 0.077 \(\pm\) 0.005 ab \\
2 & 0.020 \(\pm\) 0.001 aa & 0.025 \(\pm\) 0.000 aa & 0.053 \(\pm\) 0.005 ab \\
3 & 0.046 \(\pm\) 0.004 aa & 0.065 \(\pm\) 0.006 ba & 0.118 \(\pm\) 0.004 bb \\
4 & 0.048 \(\pm\) 0.002 aa & 0.071 \(\pm\) 0.002 ba & 0.132 \(\pm\) 0.006 bb \\
5 & 0.066 \(\pm\) 0.005 aa & 0.072 \(\pm\) 0.003 ba & 0.163 \(\pm\) 0.006 cb \\
6 & 0.050 \(\pm\) 0.005 aa & 0.068 \(\pm\) 0.010 ba & 0.326 \(\pm\) 0.019 db \\
7 & 0.066 \(\pm\) 0.023 aa & 0.039 \(\pm\) 0.013 ca & 0.145 \(\pm\) 0.011 eb \\
8 & 0.021 \(\pm\) 0.001 ba & 0.048 \(\pm\) 0.006 cb & 0.149 \(\pm\) 0.007 ec \\ \hline \end{tabular} The values represent the average \(\pm\) SD of three replicates. Mean growth rates in the same column followed by different letters are significantly different according to DMRT test at \(p\leq\) 0.05. The first letter shows the differences between the weeks of cultivation, and the second letter shows the differences between the pH values of the medium.  
\end{table} 
Table 2: Total cellulase activity of _P. melonis_ 502 under different pH values. \begin{table}
\begin{tabular}{c c c c} \hline \multirow{2}{*}{**Cultivation Period,**} & \multicolumn{3}{c}{**Activity (U mL\({}^{-1}\)) \(\pm\) SD**} \\  & \multicolumn{3}{c}{**pH**} \\ \cline{2-4}  & **5.5** & **7.0** & **8.5** \\ \hline
1 & 0.121 \(\pm\) 0.003 aa & 0.128 \(\pm\) 0.002 aa & 0.135 \(\pm\) 0.010 aa \\
2 & 0.080 \(\pm\) 0.000 ba & 0.090 \(\pm\) 0.002 ba & 0.141 \(\pm\) 0.017 ab \\
3 & 0.101 \(\pm\) 0.013 ba & 0.161 \(\pm\) 0.003 cb & 0.253 \(\pm\) 0.002 bc \\
4 & 0.068 \(\pm\) 0.003 ca & 0.084 \(\pm\) 0.001 da & 0.151 \(\pm\) 0.001 cb \\
5 & 0.115 \(\pm\) 0.005 da & 0.142 \(\pm\) 0.003 ea & 0.248 \(\pm\) 0.010 db \\
6 & 0.148 \(\pm\) 0.015 ea & 0.225 \(\pm\) 0.005 fa & 0.539 \(\pm\) 0.019 ec \\
7 & 0.136 \(\pm\) 0.006 ea & 0.097 \(\pm\) 0.012 gb & 0.156 \(\pm\) 0.003 fd \\
8 & 0.063 \(\pm\) 0.004 fa & 0.095 \(\pm\) 0.004 gb & 0.196 \(\pm\) 0.010 gc \\ \hline \end{tabular} The values represent the average \(\pm\) SD of three replicates. Mean growth rates in the same column followed by different letters are significantly different according to DMRT test at \(p\leq\) 0.05. The first letter shows the differences between the weeks of cultivation, and the second letter shows the differences between the pH values of the medium.  
\end{table} 
Table 3: Exoglucanase activity of _P. melonis_ 502 under different pH values. _oxygenum_, they also showed two peaks of cellulase activity--at days 4 and 8 of cultivation on a rotary shaker. In addition, in vitro synthesis of enzymes varies depending on the method of cultivation. High cellulase and \(\beta\)-glucosidase activity of the phytopathogen of sugar beet _Sclerotium rofsii_ was shown, the peaks of which occurred on days 9 and 10, respectively, while in a dynamic culture, the activity of enzymes was almost five times higher than in a static culture [27; 61; 62]. Regarding _Plectosphaareella_, it is known that the pH-optimum of _P. cucumeria_ for the xylanase is 6.0 [63]. Chitinases _Plectosphaareella_ sp. strain MF-1 is most active at pH 3-4 [64]. In general, it is now known that the _P. melonis_ 502 is capable of the synthesis of ethylene [19] and cellulase enzymes in vitro.