## 2 Results and Discussions  
### Total Polyphenols and Anti-Radical Activity  
The total polyphenols (TP) in _T. pratense_ and _M. sativa_ seeds and sprouts extracts was determined by the Folin-Ciocalteu spectrophotometric method and the anti-radical activity (AA) has been evaluated by DPPH\(\bullet\) free radical scavenging assay. All experiments were done in duplicates. As observed in Figure 1, a decrease in TP was noticed during the first 2 days of germination in both plant species. On the other hand, all the assessed extracts were able to reduce the radical DPPH\(\bullet\). The raw data are presented in the supplementary file (Table S1). ALF sprouts extracts exhibited an increased anti-radical activity with the on-going germination (Figure 1). AA in the fourth day of germination increase 4.37-fold compared to the activity in seeds, while in the fifth day, a decrease was registered compared with day 4. A nonlinear correlation was observed between the dynamics of the total phenols and anti-radical activity in ALF sprouts with a maximum of TP in the third day of germination (18.51 mg gallic acid eq/g DW). In contrast, in the RCV sprouts' extracts the activity against DPPH\(\bullet\) was negatively correlated with the total phenolic amount (Figure 1), probable due to the changes in polyphenols profile associated highly with anti-radical activity. The highest TP was observed in the fourth day of germination while the AA decreased from a maximum reached in the second day of germination. However, even if the TP in ALF seeds was higher than in RCV seeds (25.14 compared to 18.74 mg gallic acid eq/g DW), during germination, the total polyphenols in RCV sprouts exceeded ALF sprouts in the fourth day (23.34 compared to 10.62 mg gallic acid eq/g DW). Our results are consistent with the total polyphenols and antioxidant/anti-radical activity in kinetic investigations on germinated cereals or vegetable seeds [27; 28], Thus, similar with other study on _M. sativa_[27] showing close TP and AA values and the same trends as observed in our results, TP of ALF sprouts decreased over a 2-day period and increased over the next 2 days while the variation of the AA in ALF indicates an increase during the first 4 days of germination followed by a decline in the next day. As with other authors reporting results on germinated cereals [28], in our study for both  
Figure 1: Variation of the total phenolic (TP) content (mg gallic acid eq/g DW), the anti-radical activity (AA) as scavenging capacity of DPPH\(\bullet\) (%) and isoflav content (\(\mu\)g/g DW) in ALF and RCV sprouts during germination (sprouts samples was coded as: day 1—first day of germination; day 2—second day of germination; day 3—third day of germination; day 4—fourth day of germination; day 5—fifth day of germination). Different uppercase letters corresponding to the bars denote significant differences according to Duncan test \(p\leq 0.05\). plant species, the lowest TP was found in day 1 and day 2 during the germination process, followed by an increase during the following days. Selected isoflav with estrogenic activity (genistin, genistein, daidzin, daidzein, formononentin, glycitein, and myricetin) were quantified by [PERSON], [23] in a previous work by high resolution mass spectrometry (Table S1, Supplementary Materials). In order to highlight significant differences among the samples of ALF and RCV sprouts extracts, the resulted biochemical data were statistically processed by analysis of variance (ANOVA). Thus, according to the Duncan's test, different uppercase letters corresponding to the bars indicated significant differences (\(p<0.05\)) between the corresponding RCV and ALF samples (Figure 1). The ANOVA results indicate that TP, AA, and total isoflav content were significantly different depending on the day of germination for ALF and RCV sprouts. It is worth noting that the AA and isoflav contents corresponding to the third and fourth days of germination in ALF and RCV sprouts were significantly higher compared to the other days of germination.