## 2 Materials and Methods
### Fourier Transform Infrared Measurements  
FTIR spectra were recorded on a Thermo Scientific Nicolet iS50 FTIR spectrometer (Thermo, Waltham MA, USA) using the attenuated total reflection (ATR) method at room temperature. All spectra were collected using 32 scans with a resolution of 4 cm\({}^{-1}\). The absorbance of the buffer solution (Tris-HCl buffer solution at pH 7.40) was measured and then digitally subtracted from the FTIR spectra of HSA collected in the range of 500-4000 cm\({}^{-1}\) in the absence and presence of PDQL. Synthesis of 6-Chloro-1-(3,3-dimethyl-butanoyl)-2-(un)substituted-phenyl-2,3-dihydroquinazolin-4(1H)-one Derivatives **3a-f**  
Compound **1** and the intermediate compounds (**2a-f**) were prepared according to the reported methods [26; 27]. Their properties were as follows:  
_2-Amino-5-chlorobenzhydrazazide_ (**1**): white crystals, yield 85%; m.p. 139.6-140.5 \({}^{\circ}\)C; \({}^{1}\)H-NMR (400 MHz, DMSO-\(d_{6}\)) 8 9.60 (s, 1H), 7.48 (d, \(J\) = 2.5 Hz, 1H), 7.16 (dd, \(J\) = 8.8, 2.5 Hz, 1H), 6.74 (d, \(J\) = 8.8 Hz, 1H), 6.46 (s, 2H), 4.42 (s, 2H). _3-(Benzylideneanino)-6-chloro-2-phenyl-2,3-dihydroquinazolin-4(1H)-one_ (**2a**): light yellow solid, yield 75.3%; m.p. 193.5-194.5 \({}^{\circ}\)C; \({}^{1}\)H-NMR (600 MHz, chloroform-_d_) 8 9.20 (s, 1H), 7.93 (d, \(J\) = 2.5 Hz, 1H), 7.68-7.59 (m, 2H), 7.47-7.41 (m, 2H), 7.41-7.28 (m, 6H), 7.27-7.23 (m, 1H), 6.65 (d, \(J\) = 8.5 Hz, 1H), 6.28 (s, 1H), 4.96 (s, 1H). _6-Chloro-3-((4-methylbenzylidene)amino)-2-(p-tolyl)-2,3-dihydroquinazolin-4(1H)-one_ (**2b**): light yellow crystal, yield 63.7%; m.p. 193.3-194.1 \({}^{\circ}\)C; \({}^{1}\)H-NMR (600 MHz, chloroform-_d_) 8 9.09 (s, 1H), 7.92 (d, \(J\) = 2.4 Hz, 1H), 7.54-7.52 (m, 2H), 7.31-7.29 (m, 2H), 7.24-7.22 (m, 1H), 7.16-7.07 (m, 4H), 6.62 (d, \(J\) = 8.5 Hz, 1H), 6.23 (s, 1H), 4.89 (s, 1H), 2.35 (s, 3H), 2.30 (s, 3H). _6-Chloro-3-((4-methoxybenzylidene)amino)-2-(4-methoxyphenyl)-2,3-dihydroquinazolin-4(1H)-one_ (**2c**): light yellow crystal, yield 60.5%; m.p. 204.3-206.1 \({}^{\circ}\)C; \({}^{1}\)H-NMR (600 MHz, chloroform-_d_) 8 8.98 (s, 1H), 7.92 (d, \(J\) = 2.4 Hz, 1H), 7.57 (d, \(J\) = 8.3 Hz, 2H), 7.35 (d, \(J\) = 8.4 Hz, 2H), 7.28-7.19 (m, 1H), 6.90-6.76 (m, 4H), 6.62 (d, \(J\) = 8.5 Hz, 1H), 6.20 (s, 1H), 4.88 (s, 1H), 3.81 (s, 3H), 3.75 (s, 3H). _6-Chloro-3-((4-cyanophenylidene)amino)-2-(4-cyanophenyl)-2,3-dihydroquinazolin-4(1H)-one_ (**2d**): white crystal, yield 65.3%; m.p. 200.1-202.0 \({}^{\circ}\)C; \({}^{1}\)H-NMR (600 MHz, DMSO-\(d_{6}\)) 8 9.04 (s, 1H), 8.27 (d, \(J\) = 3.4 Hz, 1H), 7.89-7.82 (m, 6H), 7.63 (d, \(J\) = 2.5 Hz, 1H), 7.53 (d, \(J\) = 8.1 Hz, 2H), 7.37-7.32 (m, 1H), 6.86 (d, \(J\) = 8.7 Hz, 1H), 6.69 (d, \(J\) = 3.3 Hz, 1H). _6-Chloro-3-((4-trifluoromethyl)benzylidene)amino)-2-(4-(trifluoromethyl)phenyl)-2,3-dihydroquinazolin-4(1H)-one_ (**2e**): white crystal, yield 65.3%; m.p. 200.1-202.0 \({}^{\circ}\)C; \({}^{1}\)H-NMR (600 MHz, chloroform-_d_) 8 9.49 (s, 1H), 7.92 (d, \(J\) = 2.4 Hz, 1H), 7.72 (d, \(J\) = 8.0 Hz, 2H), 7.64-7.52 (m, 6H), 7.31-7.25 (m, 1H), 6.72 (d, \(J\) = 8.5 Hz, 1H), 6.34 (s, 1H), 5.02 (s, 1H). _6-Chloro-3-((4-isopropylbenzylidene)amino)-2-(4-isopropylphenyl)-2,3-dihydroquinazolin-4(1H)-one_ (**2f**): white crystal, yield 65.3%; m.p. 200.1-202.0 \({}^{\circ}\)C; \({}^{1}\)H-NMR (600 MHz, chloroform-_d_) 8 9.10 (s, 1H), 7.96-7.85 (m, 1H), 7.57 (d, \(J\) = 8.0 Hz, 2H), 7.33 (s, 2H), 7.24-7.18 (m, 3H), 7.16-7.14 (m, 2H), 6.62 (d, \(J\) = 8.5 Hz, 1H), 6.25 (d, \(J\) = 2.4 Hz, 1H), 5.11 (s, 1H), 2.88 (m, 2H), 1.27-1.15 (m, 12H). Compounds **3a-f** were prepared with the same procedure. Compound **2** (3 mmol) in anhydrous tetrahydrofuran (30 mL) was cooled to 0 \({}^{\circ}\)C using an ice bath, and then, sodium hydride (3.6 mmol) was added. The mixture was stirred first for 0.5 h at 0 \({}^{\circ}\)C and by another 1.0 h at room temperature. _tert_-Butylacetyl chloride (3.6 mmol) in anhydrous tetrahydrofuran (5 mL) was then added slowly at 0 \({}^{\circ}\)C in 0.5 h, followed by stirring the mixture at room temperature overnight.