## 2 Materials and Methods
### Behavioral Tests
\begin{table}
\begin{tabular}{c c c c c c c} \hline
**CatWalk XT® Parameters** & **BL** & **N2** & **N3** & **N4** & **N5** & **N6** \\ \hline Regularity Index (\%) & 98.03 \(\pm\) 0.80 & 0.99 \(\pm\) 0.99 & 4.63 \(\pm\) 2.26 & 6.80 \(\pm\) 3.91 & 13.94 \(\pm\) 3.10 & 9.90 \(\pm\) 3.07 \\ Average Speed (cm/s) & 28.71 \(\pm\) 1.91 & 0.80 \(\pm\) 0.46 & 2.72 \(\pm\) 0.76 & 3.95 \(\pm\) 1.04 & 10.00 \(\pm\) 1.49 & 7.87 \(\pm\) 1.36 \\ Stride Length (FL, cm) & 11.91 \(\pm\) 0.49 & 0.22 \(\pm\) 0.15 & 0.78 \(\pm\) 0.23 & 1.20 \(\pm\) 0.26 & 2.25 \(\pm\) 0.44 & 1.97 \(\pm\) 0.33 \\ Stride Length (HL, cm) & 12.16 \(\pm\) 0.37 & 0.75 \(\pm\) 0.37 & 2.26 \(\pm\) 0.53 & 2.06 \(\pm\) 0.53 & 5.54 \(\pm\) 0.89 & 5.43 \(\pm\) 0.89 \\ Base of Support (FL, cm) & 1.93 \(\pm\) 0.08 & 6.67 \(\pm\) 0.23 & 5.24 \(\pm\) 0.42 & 4.51 \(\pm\) 0.40 & 3.54 \(\pm\) 0.44 & 3.58 \(\pm\) 0.41 \\ Base of Support (HL, cm) & 2.88 \(\pm\) 0.08 & 6.46 \(\pm\) 0.26 & 5.48 \(\pm\) 0.35 & 4.83 \(\pm\) 0.33 & 4.05 \(\pm\) 0.38 & 4.26 \(\pm\) 0.31 \\ Max Intensity (FL) & 176.76 \(\pm\) 3.15 & 4.72 \(\pm\) 3.28 & 36.65 \(\pm\) 8.93 & 41.05 \(\pm\) 6.74 & 55.76 \(\pm\) 9.01 & 60.25 \(\pm\) 10.25 \\ Max Intensity (HL) & 167.80 \(\pm\) 6.25 & 13.96 \(\pm\) 6.86 & 51.69 \(\pm\) 12.00 & 54.88 \(\pm\) 10.26 & 74.23 \(\pm\) 9.90 & 67.60 \(\pm\) 9.79 \\ Mean Intensity (FL) & 78.20 \(\pm\) 1.24 & 3.70 \(\pm\) 2.56 & 24.42 \(\pm\) 5.65 & 30.59 \(\pm\) 4.79 & 45.57 \(\pm\) 5.65 & 44.85 \(\pm\) 5.70 \\ Mean Intensity (HL) & 75.19 \(\pm\) 2.45 & 8.40 \(\pm\) 4.00 & 27.56 \(\pm\) 6.15 & 35.67 \(\pm\) 5.63 & 46.57 \(\pm\) 5.88 & 44.97 \(\pm\) 5.12 \\ Max Contact Max Intensity (FL) & 161.81 \(\pm\) 3.27 & 4.94 \(\pm\) 3.44 & 38.04 \(\pm\) 9.03 & 39.23 \(\pm\) 6.38 & 63.10 \(\pm\) 8.78 & 57.83 \(\pm\) 9.10 \\ Max Contact Max Intensity (HL) & 149.50 \(\pm\) 7.72 & 11.82 \(\pm\) 5.77 & 45.01 \(\pm\) 10.49 & 49.80 \(\pm\) 8.89 & 65.63 \(\pm\) 8.59 & 56.86 \(\pm\) 8.26 \\ Max Contact Mean Intensity (FL) & 74.18 \(\pm\) 1.15 & 3.63 \(\pm\) 2.52 & 23.62 \(\pm\) 5.46 & 29.73 \(\pm\) 4.64 & 44.55 \(\pm\) 5.51 & 43.49 \(\pm\) 5.49 \\ Max Contact Mean Intensity (HL) & 69.71 \(\pm\) 2.46 & 7.90 \(\pm\) 3.76 & 26.75 \(\pm\) 5.88 & 34.00 \(\pm\) 5.32 & 49.30 \(\pm\) 5.22 & 42.15 \(\pm\) 4.74 \\ Print Length (FL, cm) & 2.10 \(\pm\) 0.02 & 0.04 \(\pm\) 0.03 & 0.32 \(\pm\) 0.08 & 0.34 \(\pm\) 0.08 & 0.45 \(\pm\) 0.08 & 0.60 \(\pm\) 0.11 \\ Print Length (HL, cm) & 2.22 \(\pm\) 0.06 & 0.12 \(\pm\) 0.06 & 0.55 \(\pm\) 0.14 & 0.38 \(\pm\) 0.11 & 0.92 \(\pm\) 0.15 & 0.83 \(\pm\) 0.16 \\ Print Width (FL, cm) & 1.97 \(\pm\) 0.03 & 0.03 \(\pm\) 0.02 & 0.25 \(\pm\) 0.06 & 0.24 \(\pm\) 0.05 & 0.37 \(\pm\) 0.06 & 0.41 \(\pm\) 0.07 \\ Print Width (HL, cm) & 2.07 \(\pm\) 0.06 & 0.12 \(\pm\) 0.06 & 0.51 \(\pm\) 0.13 & 0.39 \(\pm\) 0.10 & 0.77 \(\pm\) 0.11 & 0.69 \(\pm\) 0.12 \\ Print Area (FL, cm\({}^{2}\)) & 1.90 \(\pm\) 0.06 & 0.01 \(\pm\) 0.01 & 0.13 \(\pm\) 0.04 & 0.09 \(\pm\) 0.03 & 0.16 \(\pm\) 0.04 & 0.25 \(\pm\) 0.06 \\ Print Area (HL, cm\({}^{2}\)) & 2.06 \(\pm\) 0.15 & 0.05 \(\pm\) 0.03 & 0.45 \(\pm\) 0.15 & 0.29 \(\pm\) 0.12 & 0.64 \(\pm\) 0.14 & 0.55 \(\pm\) 0.14 \\ Max Contact Area (FL, cm\({}^{2}\)) & 1.52 \(\pm\) 0.05 & 0.01 \(\pm\) 0.01 & 0.10 \(\pm\) 0.03 & 0.06 \(\pm\) 0.01 & 0.12 \(\pm\) 0.03 & 0.25 \(\pm\) 0.07 \\ Max Contact Area (HL, cm\({}^{2}\)) & 1.59 \(\pm\) 0.13 & 0.03 \(\pm\) 0.02 & 0.19 \(\pm\) 0.05 & 0.14 \(\pm\) 0.06 & 0.37 \(\pm\) 0.08 & 0.30 \(\pm\) 0.08 \\ Max Intensity at \% (\%\) (FL) & 66.83 \(\pm\) 1.36 & 2.02 \(\pm\) 1.48 & 19.19 \(\pm\) 4.34 & 17.41 \(\pm\) 3.22 & 25.10 \(\pm\) 3.90 & 28.86 \(\pm\) 3.92 \\ Max Intensity at \% (\%\) (HL) & 63.12 \(\pm\) 2.94 & 6.75 \(\pm\) 3.23 & 23.09 \(\pm\) 4.76 & 18.22 \(\pm\) 3.19 & 34.48 \(\pm\) 3.99 & 33.63 \(\pm\) 4.13 \\ Max Contact at \% (FL) & 45.75 \(\pm\) 0.88 & 2.09 \(\pm\) 1.56 & 17.19 \(\pm\) 4.22 & 16.65 \(\pm\) 3.20 & 24.15 \(\pm\) 3.92 & 30.19 \(\pm\) 4.37 \\ Max Contact at \% (\%\) (HL) & 25.32 \(\pm\) 1.63 & 4.77 \(\pm\) 2.35 & 16.77 \(\pm\) 3.71 & 13.99 \(\pm\) 2.51 & 21.61 \(\pm\) 2.51 & 21.62 \(\pm\) 2.78 \\ Swing Speed (FL, cm/s) & 86.74 \(\pm\) 3.81 & 1.62 \(\pm\) 1.21 & 14.07 \(\pm\) 4.01 & 16.54 \(\pm\) 4.76 & 26.50 \(\pm\) 4.61 & 34.82 \(\pm\) 6.15 \\ Swing Speed (Following the CatWalk XT\({}^{\text{\textregistered}}\) automated gait analysis, the BBB open field rating scale score was performed in all animals at the same timepoints (BL, N2-N6) to assess the general recovery of hind limb locomotion [13].