# Effects of Different Extraction Methods on Vanilla Aroma
## 2 Results
### Investigation of the Effect of Different Extraction Methods on the Aroma Components of Vanilla Pods
#### 2.1.2 HS-SPME
\begin{table}
\begin{tabular}{l c c c c c c c} \hline \hline  & & \multicolumn{6}{c}{**Peak Areas \({}^{\text{3}}\)**} \\ \cline{3-8}
**Compounds \({}^{\text{1}}\)** & **RI \({}^{\text{2}}\)** & \multicolumn{6}{c}{**SE**} \\ \cline{3-8}  & & \multicolumn{2}{c}{**35\% Ethanol**} & **75\% Ethanol** & **95\% Ethanol** & **SD** & **HS-SPME** \\ \cline{3-8} ethyl acetate & 601 & 24.34 \(\pm\) 9.04 & - \({}^{\text{4}}\) & - & - & - & - \\
3-methylbutanal & 627 & - & - & - & - & 873.24 \(\pm\) 113.12 & - \\
3-methylpentanal & 740 & - & - & - & - & 306.10 \(\pm\) 145.40 & - \\ hexanal & 772 & - & - & - & - & 846.53 \(\pm\) 16.78 & - \\
1,3-butanedid & 777 & - & - & - & - & 117.55 \(\pm\) 18.55 \\ furfural & 790 & 41.05 \(\pm\) 9.58c & - & - & 20.32 \(\pm\) 3.09c & 159.07 \(\pm\) 6.22a & 127.28 \(\pm\) 15.98b \\ furfuryl alcohol & 844 & - & 27.62 \(\pm\) 273a & 33.76 \(\pm\) 14.29a & 37.67 \(\pm\) 10.91a & - & - \\ heptanal & 874 & - & - & - & 128.62 \(\pm\) 16.41 & - \\
5-methyl-2(5H)-furanone & 886 & - & - & 24.69 \(\pm\) 11.47 & - & - & - \\
5-methylfurfurral & 921 & - & 7.44 \(\pm\) 0.70b & 10.19 \(\pm\) 1.22a & - & - & - \\ benzaldehyde & 922 & 25.94 \(\pm\) 5.46c & - & - & 246.10 \(\pm\) 6.85a & 84.20 \(\pm\) 13.60b \\ phenol & 947 & 151.63 \(\pm\) 31.97b & 11.52 \(\pm\) 2.05c & 25.65 \(\pm\) 2.86c & 14.92 \(\pm\) 19.7c & - & 428.03 \(\pm\) 52.91a \\
1-octen-3 one & 948 & - & - & - & - & 61.90 \(\pm\) 4.79 & - \\
2-octanone & 954 & - & - & - & - & 203.83 \(\pm\) 28.07 & - \\
1-octen-3 ol & 955 & - & - & - & - & 47.87 \(\pm\) 11.27b & 279.51 \(\pm\) 53.18a \\
2-methylfuran & 968 & 16.11 \(\pm\) 4.99b & - & - & - & 289.08 \(\pm\) 59.24a \\ octanan & 971 & - & - & - & - & 265.90 \(\pm\) 59.40 & - \\ hexanoic acid & 975 & 60.46 \(\pm\) 37.02 & - & - & - & - & 114.85 \(\pm\) 14.82a \\ benzyl alcohol & 992 & 22.11 \(\pm\) 6.29b & - & - & - & 17.18 \(\pm\) 3.86b & - \\ phenylacetaldehyde & 996 & 39.00 \(\pm\) 8.68a & - & - & - & 17.18 \(\pm\) 3.86b & - \\
3-octen-2 one & 999 & 15.71 \(\pm\) 4.24c & - & - & - & 221.40 \(\pm\) 19.36a & 108.05 \(\pm\) 15.40b \\ imione & 1010 & 13.19 \(\pm\) 4.09a & - & - & - & 24.58 \(\pm\) 5.86a & \\ furnaceel & 1011 & - & - & - & 9.41 \(\pm\) 4.18 & - & - \\ _p_-cresol & 1037 & 50.37 \(\pm\) 13.50b & 4.75 \(\pm\) 0.52c & 14.09 \(\pm\) 5.72c & 10.87 \(\pm\) 2.95c & - & 144.94 \(\pm\) 20.72a \\ _1_-octanol & 1041 & 117.30 \(\pm\) 30.42b & - & - & 321.86 \(\pm\) 37.59a & 380.32 \(\pm\) 40.93a \\ guaiocol & 1052 & 1747.13 \(\pm\) 405.33b & 11.34 \(\pm\) 0.97c & 24.61 \(\pm\) 2.28c & 24.41 \(\pm\) 5.69c & 175.93 \(\pm\) 11.37c & 5315.06 \(\pm\) 911.24a \\
2-nonanone & 1059 & - & - & - & - & 851.8 \(\pm\) 70.9a & 96.07 \(\pm\) 11.61a \\ nonanal & 1070 & 31.47 \(\pm\) 10.74b & - & - & - & 333.49 \(\pm\) 41.50a & 88.34 \(\pm\) 12.02b \\ _2_-phenylethanol & 1073 & 17.35 \(\pm\) 4.16b & - & - & - & - & 42.07 \(\pm\) 1.94a \\
2-(1-methylfuylyl- & - & - & - & - & - & 55.54 \(\pm\) 6.39 & - \\ deneycycolehavenone & 1088 & - & - & - & - & - & 35.06 \(\pm\) 3.14 & - \\ methyl octanoate & 1091 & - & - & - & - & - & 15.84 \(\pm\) 1.59 \\
1-2-dimethoxybenzene & 1096 & - & - & - & - & - & 15.84 \(\pm\) 1.59 \\
2-3-dihydro-3-5-5. & & - & - & - & - & - \\ dibhydroxy-methyl-4H- & 1102 & - & 81.02 \(\pm\) 18.00a & 85.59 \(\pm\) 17.35a & 75.80 \(\pm\) 25.06a & - & - \\ pyran-4o & 1122 & - & - & 12.68 \(\pm\) 3.87 & - & - & - \\
3-5-dimethylphenol & 1131 & 8.25 \(\pm\) 3.90 & - & - & - & - & - \\ octanoic acid & 1144 & 194.34 \(\pm\) 50.37 & - & - & - & - & - \\
2-nonneal & 1151 & & - & - & - & 148.2  
Compared with other extraction methods, HS-SPME extracted more monoterpenes and sesquiterpenes. Although the total peak area of HS-SPME was highest, no carboxylic acid compounds were identified, and the types of compounds were lower than those obtained with SDE. [PERSON] et al. (2018) found that HS-SPME was more suitable for compounds with low volatility due to the lower extraction temperature.