# FhMYB108 Regulates the Expression of Linalool Synthase Gene in _Freesia hybrida_ and _Arabidopsis_
## 3 Results  
### Composition and Release Regulations of Volatile Compounds in F. hybrida  
The blossoming of flowers is accompanied by the release of a plethora of volatile compounds, functioning as chemical cues to attract insects for the purpose of pollination. Here, the GC-MS analysis revealed that the volatile compounds emitted during the blooming phase of _Red River(r)_ flowers were predominantly composed of 18 monoterenes, 5 sesquiterpenoids, and 3 carotenoid derivatives (Table 1). Among them, the monoterenes constituted 82.41% of the content, whereas carotenoid derivatives accounted for 16.84%, and sesquiterpenes contributed a mere 0.75% (Figure 1A). The results of further qualitative analysis revealed that the volatile compounds identified in the sample were primarily composed of linalool (44.83%), followed by terpineol (22.88%). Additionally, a certain quantity of limonene (D-limonene) (5.05%), ionone (\(\beta\)-Ionone) (12.66%), dihydroionone (dihydro-\(\pi\)Ionone) (3.15%), myrcene (2.66%), and basil (E)-ocimene) were detected (Figure 1B). \begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Categories** & **RT (min)** & **Compounds** & **Formula** & **Relative Contents** \\ \hline \multirow{8}{*}{Monoterpenses} & 5.036 & \(\alpha\)-Pinene & C\({}_{10}\)H\({}_{16}\) & 0.25\% \\  & 6.089 & Sabinene & C\({}_{10}\)H\({}_{16}\) & 0.42\% \\  & 6.182 & \(\beta\)-pinene & C\({}_{10}\)H\({}_{16}\) & 0.15\% \\  & 6.476 & Dehydrocineole & C\({}_{10}\)H\({}_{16}\)O & 0.06\% \\  & 6.662 & Myrcene & C\({}_{10}\)H\({}_{16}\) & 2.67\% \\  & 7.413 & \(\alpha\)-Terpinene & C\({}_{10}\)H\({}_{16}\) & 0.89\% \\  & 7.707 & Eucalyptusptol & C\({}_{10}\)H\({}_{18}\)O & 0.69\% \\  & 7.865 & D-Limonene & C\({}_{10}\)H\({}_{16}\) & 5.05\% \\  & 8.138 & trans-Ocimene & C\({}_{10}\)H\({}_{16}\) & 1.49\% \\  & 8.458 & Terpinene & C\({}_{10}\)H\({}_{16}\) & 0.27\% \\  & 8.985 & cis-Linaloloxide & C\({}_{10}\)H\({}_{18}\)O\({}_{2}\) & 0.28\% \\  & 9.418 & Furanoid linalool oxide & C\({}_{10}\)H\({}_{18}\)O\({}_{2}\) & 0.24\% \\  & 9.629 & Terpinolene & C\({}_{10}\)H\({}_{16}\) & 0.39\% \\  & 9.922 & Linalool & C\({}_{10}\)H\({}_{18}\)O & 44.83\% \\  & 12.028 & 4-Terpineol & C\({}_{10}\)H\({}_{18}\)O & 0.32\% \\  & 12.407 & \(\alpha\)-Terpineol & C\({}_{10}\)H\({}_{18}\)O & 22.88\% \\  & 12.726 & Decanal & C\({}_{10}\)H\({}_{20}\)O & 0.31\% \\  & 12.872 & \(\beta\)-Cyclocitral & C\({}_{10}\)H\({}_{16}\)O & 0.38\% \\ \hline \multirow{4}{*}{Sesquirerpenses} & 16.077 & Cycloisoisuere & C\({}_{15}\)H\({}_{24}\) & 0.16\% \\  & 16.212 & Copenene & C\({}_{15}\)H\({}_{24}\) & 0.04\% \\  & 17.163 & Farmesene & C\({}_{15}\)H\({}_{24}\) & 0.11\% \\  & 17.627 & g-Gurjunene & C\({}_{15}\)H\({}_{24}\) & 0.19\% \\  & 18.867 & Nerodilol & C\({}_{15}\)H\({}_{26}\)O & 0.24\% \\ \hline \multirow{4}{*}{Carotonoid derivatives} & 16.875 & Dihydro-ionone & C\({}_{13}\)H\({}_{22}\)O & 3.15\% \\  & 17.525 & \(\beta\)-Ionone & C\({}_{13}\)H\({}_{20}\)O & 12.06\% \\ \cline{1-1}  & 16.699 & Ionone & C\({}_{13}\)H\({}_{20}\)O & 0.14\% \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Qualitative and quantitative analysis of volatile terpen in _Red River(r)_ flowers. Figure 1: Diverse volatile terpenoids and corresponding volatilization regulations in _F. hybrida_. (**A**) The relative proportions of monoterpenses, sesquiterpenes, and carotenoid derivatives in the volatile compounds emitted by the _Red River(r)_ flowers. (**B**) The proportion of the main volatile monomer substance in the _Red River(r)_ flowers. (**C**) The release levels of volatiles in different blooming stages of _Red River(r)_. (**D**) The release levels of linalool, terpineol, and other monoterpenses in different flowering stages. In (**C**,**D**), data are shown as the mean \(\pm\) SD of three biological replicates. By the monitoring of volatile compounds emission at various stages of flower blooming in _F. hybrida_, it becomes evident that the quantity of released volatile substances gradually increases as fragment orchid flowers unfold (Figure 1C).