## 3 Results
### Quality Assessment
\begin{table}
\begin{tabular}{c c c c} \hline \hline
**Article** & **Cell Type** & **Test Performed** & **Effects** \\ \hline [PERSON] et al. [11] & Human osteoblast & ALP, LDH and H3-thymidine & Increased osteoblast differentiation \\  & & incorporation & \\ \hline [PERSON] et al. [12] & Human osteoblasts (hFOB.19) & Electronic cell counting and WST1 & Similar to the control \\ \hline [PERSON] et al. [14] & hMSCs & ALP, cell morphology & Increased ALP activity; smaller cell area \\ \hline [PERSON] et al. [15] & Osteoblast-like (MG-63) & WST1, ALP, Type 1 collagen, OSC & Increased osteoblast differentiation \\  & & and Cell morphology & \\ \hline [PERSON] et al. [16] & Osteoblast and osteoclast & WST1, ALP, Type 1 collagen, OPG, & \\  & & TRAP, RANKL and Cell & Increased osteoblast differentiation \\  & & morphology & \\ \hline [PERSON] et al. [17] & Osteoblast-like (MG-63) & WST1, LDH, ALP, Type 1 collagen, OSC and cell morphology & Increased osteoblast differentiation \\ \hline [PERSON] a et al. [18] & Osteoblast-like & WST1, ALP, Type 1 collagen, OSC, & Decreased formation of \\  & (MG63)/Osteoclasts (human) & OFFG OSC, TRAP and cell morphology & \\ \hline [PERSON] et al. [19] & Human endothelial cells (ECV304) & SEM, MTT, migration activity & Increased migration and proliferation \\ \hline [PERSON] et al. [20] & MC3T3-E1 osteoblasts & Cell attachment, proliferation & Enhanced cell attachment, proliferation, \\  & & (CCK-8 assay), differentiation & increased ALP activity, and upregulated \\  & & (ALP activity, RT-PCR for Col I, & expression of osteogenic markers (Col I, \\  & & Rumx-2, OPN, and Osterix) & Runx-2, OPN, and Osterix) \\ \hline [PERSON] et al. [21] & RAW 264.7 macrophages, mMSCs & Cell proliferation (Alamarblue & Enhanced osteogenic differentiation, \\  & & assay), cell morphology (CLSM, & inhibited osteoclastic differentiation, \\  & & SEM), gene expression (RT-PCR), & upregulated osteogenic gene expression, \\  & & protein expression (ELISA, & downregulated osteoclast-specific protein \\  & & Western blot) & activity (TRAP, CAII) \\ \hline [PERSON] et al. [22] & MC3T3-E1 osteoblast/RAW264.7 & MTT and cell morphology. & Decreased formation of \\  & & osteoclast & \\ \hline [PERSON] et al. [6] & Balb/c 3T3 fibroblasts/Primary human osteoblasts & XTT, NR, CVDE, apoptosis & \\  & & induction and cell morphology & Increased osteoblast differentiation \\  & & and adhesion. & \\ \hline [PERSON] et al. [23] & Murine macrophage & & Increased macrophage-mediated \\  & (RAW264.7)/ rabbit osteoclasts & MTT, TRAP and SEM & degradation/inhibited osteoclasts activity \\ \hline [PERSON] et al. [24] & Endothelial cells/primary human osteoblasts & MTT, TLS & Increased proliferation and TLS formation \\ \hline [PERSON] et al. [25] & MC3T3-E1 osteoblasts & Cell proliferation (CCK-8 assay), ALP activity, fluorescent staining & Enhanced proliferation, higher ALP \\ \hline [PERSON] et al. [26] & MG63 human osteoblast-like cells & Direct biocompatibility & High viability for indirect \\  & (PrestoBlue® assay, Thermo Fisher & biocompatibility; direct biocompatibility \\  & & Scientific, Waltham, MA, USA), & affected by paste/gel disaggregation, \\  & & indirect biocompatibility & highest viability observed with 0 and 100 \\  & (PrestoBlue® assay) & at.\% SrHA \\ \hline [PERSON] et al. [27] & Human fibroblasts & MTT, Alamar blue, LDH, and & Increased cytotoxicity \\  & & SEM & \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Parameters evaluated on the in vitro studies. \begin{table}
\begin{tabular}{c c c c} \hline \hline
Most in vitro tests performed at least two assays, covering both viability and cell differentiation analysis. The exception was the articles by [PERSON] et al. (2019), [PERSON] et al. (2019), and [PERSON] et al. (2020), which performed only the MTT test (Table 3). However, all these studies performed in vivo tests, which presented analyses of bone regeneration. [PERSON] et al. (2020) also presented molecular tests that could replace the in vitro tests of cell differentiation.