## 3 Results and Discussion
### Further Metabolite Investigations of Different P. mauritianum Specimens  
The chemical composition of the _P. mauritianum_ voucher specimen is in accordance with previous reported studies. However, phloroglucinols (namely aspidin VB and aspidin \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Compound** & \begin{tabular}{c} **Antiviral Activity** \\ **IC\({}_{50}\) (uM)** \\ \end{tabular} & \
\begin{tabular}{c} **Cell Toxicity** \\ **CC\({}_{50}\) (uM)** \\ \end{tabular} &
\begin{tabular}{c} **Selectivity index SI** \\ **(CC\({}_{50}\)/IC\({}_{50}\))** \\ \end{tabular} \\ \hline Positive control Q3G & 4.22 \(\pm\) 0.11 & 96.26 \(\pm\) 0.07 & 22.81 \\ Corosolic acid (**10**) & 26.80 \(\pm\) 6.80 & 31.14 \(\pm\) 0.03 & 1.16 \\ Betulinic acid (**11**) & 20.21 \(\pm\) 0.10 & 22.40 \(\pm\) 0.09 & 1.11 \\ Maslinic acid (**9**) & 82.97 \(\pm\) n/a & 84.57 \(\pm\) 0.04 & 1.02 \\ Asiatic acid+ Arjulonic acid (**5** + 6) & 98.57 \(\pm\) 0.21 & 73.07 \(\pm\) 0.06 & 0.74 \\ New compound (**1**) & 70.28 \(\pm\)1.48 & 41.93 \(\pm\) 0.08 & 0.60 \\ Betulinic acid (**12**) & 57.55 \(\pm\) 0.31 & 24.47 \(\pm\) 0.05 & 0.43 \\ Alphtiolic acid (**8**) & \(>\)100 & - & - \\ Astragalim (**13**) & \(>\)100 & - & - \\ Actinidic acid (**4**) & \(>\)100 & - & - \\ Terminolic acid + Madecassic acid (**2** + 3) & \(>\)100 & - & - \\
3b,23-dihydroxy-1-oxo-olean-12-en-28- & \(>\)100 & - & - \\ oic acid (**7**) & \(>\)100 & - & - \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Antiviral activity (ZIKV in Vero cells) and cell toxicity of isolated triterpenenes. A higher SI means better suitability against the virus. BB) [5], usually reported in the different published phytochemical investigation of the plant extracts, were missing. As these compounds have been reported to have potent antibacterial activity, which is one of the purposes of the traditional use of this plant species, we wanted to investigate if the extract chemical composition may vary according to the sample location. Four other specimens of _P. mautiianum_ morphologically similar to the voucher specimen were collected at a different location (Basse Vallee, 25 km from the voucher's site) on the same day and were all found within a radius of 10 m. The samples were prepared and analysed as previously, following the same extraction protocol and the same metabolomic profiling by UPLC-HRMS/MS to enable a proper comparison with the voucher specimen. To this end, MS2 data were analysed through a newly generated IIMN (Figure 6) to display the metabolomic differences between the different _P. mautiianum_ specimens. The metabolite content of the five specimen extracts was rather heterogeneous. If methoxylated flavonoids (e.g., compound **14**, cluster A4) and triterpenes (e.g., betulinic acid, cluster A2) previously described are present in all analysed samples, some compounds are found only in one or some of the specimens. For instance, psiloxylic acid (**1**), described for the first time in the current work, is specific to the voucher specimen. As all samples were processed and analysed the same way, psiloxylic acid is unlikely to be an extraction artefact derived from betulinic acid. Presumably, the voucher's site, which has a lower altitude and is on a drier range of the mountain, favored the biosynthesis of psiloxylic acid. Interestingly, the phloroglucinols aspidin VB and aspidin BB were present in two samples analysed out of five. (Some traces could be found in a third sample). These two compounds are absent from the voucher's sample but also from two specimens out of four having the same abiotic factors. This underlines the chemical phenotype of _P. mautiianum_ does not depend solely on climate and location and hypothetically is affected by genetic variability [26], individual development, or endophytic relationship [27; 28]. Little is known about these aspects for plants from La Reunion, and further work needs to be conducted to investigate _P. mautiianum_ chemical variability. Figure 6: IIMN of the fractioned _P.