## 2 Metallic Nanoparticles in Conjugation with Chlorin Derivatives
### Iron Oxide and Other Magnetic Nanoparticles
The authors observed no toxic effect against _Daplinnia magna_ under the same conditions used against mosquitos. Water plants _Pseudokirchneriella subcapitata_ and _Lenna minor_ were also treated with the developed nanoparticles, and again, no impact on their growth was noted [26]. The data described in Section 2.1 are summarized in Table 1.  
**In summary:**  
* Most magnetic nanoparticles used were Fe\({}_{3}\)O\({}_{4}\) conjugated with Ce6 via silica or dextran coatings or incorporated into a polymer structure. * The conjugates of chlorins and magnetic nanoparticles allowed for efficient combined magnetothermal-photodynamic treatment after laser irradiation at 660 and 808 nm. * The promising theranostic features of the Fe\({}_{3}\)O\({}_{4}\)-chlorin conjugates can be used for photodynamic therapy and MRI or fluorescent imaging. * Different types of connections in the obtained conjugates were used, including core-shell structures, liposomes, polymeric nanoclusters, and covalent bonding. \begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline
**Type of NPs** & **Size of NPs** & \begin{tabular}{c} **Preparation** \\ **Method** \\ \end{tabular} & \begin{tabular}{c} **Wavelength** \\ **[nm]** \\ \end{tabular} & \begin{tabular}{c} **Light** \\ **Dose/Light** \\ **Power** \\ \end{tabular} & **Cell Type** & **Cell Viability** & **Ref.** \\ \hline Fe\({}_{3}\)O\({}_{4}\) (loaded in liposomes) & 150 nm & \begin{tabular}{c} alkaline \\ co-precipitation of \\ FeCl\({}_{2}\) and FeCl\({}_{3}\) \\ \end{tabular} & 650 & 5 or 10 J/cm\({}^{2}\) & \begin{tabular}{c} human \\ adenocarcinoma \\ SKOV-3 cells \\ \end{tabular} & \begin{tabular}{c} 0.2\% at \\ 5 J/cm\({}^{2}\) and \\ 0\% at 10 J/cm\({}^{2}\) \\ \end{tabular} & [16] \\ \hline Fe\({}_{3}\)O\({}_{4}\) & 20 nm & \begin{tabular}{c} coprecipitation of \\ Fe\({}^{2+}\) and Fe\({}^{3+}\) \\ in the presence of \\ NH\({}_{3}\times\) H\({}_{2}\)O \\ \end{tabular} & 632.8 & 30 mW/cm\({}^{2}\) & \begin{tabular}{c} MIC803 cells \\ 80\% at 42 J\({}^{2}\)M \\ \end{tabular} & [17] \\ \hline Fe\({}_{3}\)O\({}_{4}\) & 92 nm & \begin{tabular}{c} thermal \\ decomposition \\ method \\ \end{tabular} & 665 & 5 J/cm\({}^{2}\) & 4T1 cells & \textless{}10\% & [18] \\ \hline Fe\({}_{3}\)O\({}_{4}\) & 90 nm & \begin{tabular}{c} thermal \\ decomposition \\ method \\ \end{tabular} & 660 & N/A & MDA-MB-231 cells & approx. 20\% & [19] \\ \hline Fe\({}_{3}\)O\({}_{4}\) & 220 \(\pm\) 30 nm & \begin{tabular}{c} reaction of \\ iron with \\ triethylene glycol \\ at 300 \({}^{\circ}\)C for 2 h \\ \end{tabular} & 690 & 0.5 W/cm\({}^{2}\) & Lewis cells & approx. 0.5\% & [20] \\ \hline Dextran-coated & 49.9 nm & \begin{tabular}{c} crosslinking of the \\ dextran coating \\ around the iron \\ oxide core \\ \end{tabular} & 650 & 150 mW/cm\({}^{2}\) & \
\begin{tabular}{c} murine \\ macrophage RAW \\ (6 J/cm\({}^{2}\)) \\ \end{tabular} & 0\% (LD_{8}\) of [21] \\ \hline Aptamer and & 10 nm &
\begin{tabular}{c} thermal \\ decomposition \\ method \\ \end{tabular} & 660 and 808 & 100 mW/cm\({}^{2}\) & MCF-7 tumor cells & 16.33\% & [22] \\ \hline \hline \end{tabular}
\end{table} 
Table 1: A summary of the data presented in Section 2.1.