# Analysis of Disinfection in Greenhouse Soils with Medium-Temperature Steam Produced by Solar Energy
## 4 Analysis of the Results of Disinfected Soils  
After testing the soils, biological analysis of the samples was carried out, using the methodology mentioned in Section 2.1. Table 15 shows the results of the cucumber soil analyses, starting from the control (or untreated) soil for each dilution. Table 15 shows the microorganisms present in the various treatments in the control soil where cucumber cultivation was established (_Cucumis sativus_ L.), and Table 16 shows the results of the analysis of tomato soils. The presence of microorganisms such as _Cladosporium_ sp. was observed. It is a genus of fungi that includes some of the most common indoor and outdoor molds. The species produce olive to brown or black colonies and have dark pigmented conidia that form in single or branched chains. Many species of the genus _Cladosporium_ are commonly found in living and dead plant material. Some species are plant pathogens, while others parasitize other fungi [46]. _Cladosporium_ spores are dispersed by wind and are often extremely abundant in the outside air. Indoors, _Cladosporium_ species can grow on surfaces when there is moisture. _Cladosporium fulvum_, the cause of tomato leaf mold, has been an important genetic model, in which the genetics of host resistance are understood as _Aspergillus niger_, a species of fungus harmless to humans and also to most crops [22]. The natural chemical compounds of phosphorus are unhealthy. \begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Treatment** & **Dilution** & **N of CFU** & **Microorganism** \\ \hline \multirow{2}{*}{\begin{tabular}{c} Cucumber 1 \\ (unvaporized sample) \\ \end{tabular} } & \(10^{-3}\) & 140 & _Cladosporium_ sp. \\  & \(10^{-6}\) & - & - \\  & \(10^{-10}\) & - & - \\ \hline \multirow{2}{*}{\begin{tabular}{c} Cucumber 1 \\ 1 day, 8 cycles \\ \end{tabular} } & \(10^{-3}\) & 9 & \multirow{2}{*}{_Aspergillus niger_, yeast} \\  & \(10^{-10}\) & - & \\ \hline \multirow{2}{*}{\begin{tabular}{c} Cucumber 1 \\ 1 day, 8 cycles \\ \end{tabular} } & \(10^{-3}\) & - & _Cordycps_ sp. (white xylems) \\  & \(10^{-6}\) & - & - \\  & \(10^{-10}\) & - & Possible _Xylaria_ sp. \\ \hline \multirow{2}{*}{\begin{tabular}{c} Cucumber 2 \\ 2 days, 16 cycles \\ \end{tabular} } & \(10^{-3}\) & 50 & Penicillin \\  & \(10^{-6}\) & 29 & Possible Xylaria sp. \\  & \(10^{-10}\) & - & - \\ \hline \multirow{2}{*}{\
\begin{tabular}{c} Cucumber 2 \\ 2 days, 16 cycles \\ \end{tabular} } & \(10^{-3}\) & 54 & _Cladosporium_ sp., _Penicillin_ sp. \\  & \(10^{-6}\) & 14 & _Penicillium_ sp. (green rings) \\  & \(10^{-10}\) & 250 & Yeasts sp. \\ \hline \multirow{2}{*}{
\begin{tabular}{c} Cucumber 2 \\ 2 days, 16 cycles \\ \end{tabular} } & \(10^{-3}\) & 22 & _Cladosporium_ sp., _Penicillin_ sp. \\  & \(10^{-6}\) & 35 & _Cladosporium_ sp., _Penicillin_ sp. \\  & \(10^{-10}\) & 20 & _Cladosporium_ sp., _Penicillin_ sp. \\ \hline \hline \end{tabular}
\end{table} 
Table 14: Test data 3. Cherry tomato soil. Data as of 18 February 2022. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Trial** & **Environmental** & **Start Time** & **End Time** & **Warm Up Time** & **Pressure** & **Steam Output** \\  & **Temp. ** (\({}^{\circ}\)C) & **(hh:mm)** & **(hh:mm)** & **(min)** & **Reached (psi)** & **Time (s)** \\ \hline
1 & 21 & 10:45 & 10:57 & 12 & 80 & 115 \\
2 & 21 & 11:00 & 11:10 & 10 & 80 & 138 \\
3 & 21 & 11:13 & 11:23 & 10 & 80 & 142 \\
4 & 21 & 11:26 & 11:37 & 11 & 80 & 153 \\
5 & 22 & 11:45 & 12:56 & 11 & 80 & 152 However, in the case of vegetables, they are an essential nutrient. _Xylaria_ sp. is a sapprobial fungus, which feeds on dead or decomposing organic matter and belongs to the genus of fungi of the Xylariaceae family; it usually prefers to develop on decomposing wood. Its name derives from the Greek word xylon, which means wood. Being a soil from forests, there might have been some ascopores that remained in a state of dormancy, and when humidity conditions were present, they proliferated.