###### Abstract
Based on a comprehensive analysis of the transcriptome data from 11 fungi along with four related environmental factors, large fungi were concluded to share a common pathway that likely plays a crucial role in primordium formation; for example, the MAPK pathway and the Hedgehog signaling pathway played significant regulatory roles in the light-induced formation of primordia [7; 8], while some genes encoding heat shock proteins, the G protein, and 8-1,3-glucanase in the GH5 family were involved in the fruiting body development process of _Lentinus edodes_[9]. Studies have shown that the genes involved in the formation of fruiting bodies in _Agaricus bisporus_ include the _AtpD_ gene encoding an ATP synthase subunit, the _CypA_ gene encoding a cytochrome p450, and the _SepA_ gene encoding a membrane protein, which are highly expressed during the formation of fruiting bodies [10]. Hydrophobic proteins also play a crucial role in the development of the fruiting body of _Agaricus bisporus_ As2796 [11]. Mannitol is the main means of carbon storage in _A. bisporus_, which is of great significance for its growth, fruit development, and stress tolerance. The expression of NADP-dependent mannitol dehydrogenase (_MLDH_), which controls mannitol synthesis under salt stress, was induced, leading to mannitol accumulation [12]. By integrating transcriptomic and metabolomic data, we can gain a holistic understanding of the molecular networks driving the growth and development of Caoyuanheimo-1. This systems biology approach allows for the identification of key genes, enzymes, and metabolites involved in important biological processes, facilitating targeted interventions to improve fungal cultivation, optimize the production of bioactive compounds, and harness the potential of fungi for various biotechnological applications. Terpenoids are a large family of secondary metabolites widely distributed in nature [13; 14; 15; 16]. These compounds and their derivatives, composed of isoprene (C5) units, can be classified into monoterenes (C10), sesquiterpenenes (C15), diterpenenes (C20), triterpenenes (C30), and polyterpenses based on the number of carbon atoms [17]. Studies have shown that terpenoids in plants have various functions, such as defense, disease resistance, allediopathy, and insect induction [18; 19; 20; 21; 22]. Research has also been conducted on terpenoid compounds in fungi [23; 24; 25; 26; 27], such as tetracyclines and pleuromutilin (Drosophilin B) [28; 29]. In addition, terpenoids have important medicinal functions [30; 31; 32; 33]. The biosynthetic pathways of terpenoids have been clearly studied in plants, where terpenoids are synthesized independently through two pathways. One is the membrane-associated pathway (MVA) located within the cytoplasm [34; 35], and the other is the 1-deoxy-D-xylose-5-phosphate pathway (DXP) [36] or methylerythnitol 4-phase pathway (MEP) located within the cytoplasm [37]. The biosynthesis of terpenoids in fungi has also been reported on [38; 39; 40; 41]. Based on this, genetic factors are assumed to play a key role in the environmental influences on Caoyuanheimo-1 in the process of primordium formation, fruiting body development, and maturation. At the same time, metabolites in different stages of development also show differences, including terpenoids. Therefore, the transcriptomic and metabolomic studies in this article aim to reveal the molecular mechanism of Caoyuanheimo-1's growth and development, which will lay a foundation for its efficient development and utilization.