## 3 Results and Discussion
### Physiological and Chemotaxonomic Characteristics
The total fatty acid profile (Table 2) revealed that _iso_-C\({}_{15.0}\) (23.0%) was the major component, and branched fatty acids accounted for 69% in strain WLY-B-L8T (_iso_-C\({}_{15.0}\) 23.0%, _iso_-C\({}_{17.0}\) 8.9%, _iso_-C\({}_{13.0}\) 7.9%, _iso_-C\({}_{17.1}\)\(\omega\)5 7.0%, _anteiso_-C\({}_{13.0}\) 6.3%, _iso_-C\({}_{12.0}\) 4.8%, _C\({}_{18.3}\)\(\omega\)6c 4.3%, _C\({}_{16.0}\) 3.9%, and _iso_-C\({}_{16.0}\) 3.5%). This profile differs from that of _Bacillus rhizoplanue_ JJ-63 DSM 12442T, _Bacillus pseudomycoides_ DSM 12442T, and _Bacillus cytotoxicus_ NVH 391-98T, which predominantly comprised _iso_-C\({}_{15.0}\) (19.9%), _iso_-C\({}_{17.0}\) (16.9%), and _anteiso_-C\({}_{15.0}\) (10.6%); _iso_-C\({}_{15.0}\) (13.3%), _iso_-C\({}_{13.0}\) (12.6%), and C\({}_{16.1}\)\(\omega\)6c (12.3%); _iso_-C\({}_{15.0}\) (38.6%), summed feature 3 (11.5%), and _iso_-C\({}_{17.0}\) (11.4%); respectively. It can be seen from the fatty acid results that the first class fatty acid of the strain WLY-B-L8T and the most closely related bacteria is _iso_-C\({}_{15.0}\). The results are consistent with previous reports, in which _iso_-C\({}_{15.0}\), _anteiso_-C\({}_{15.0}\), _iso_-C\({}_{16.0}\), _iso_-C\({}_{17.0}\), and _anteiso_-C\({}_{17.0}\) represent the major fatty acids typically found in _Bacillus_ species [(29)]. The differences between the three strains indicate that novel strain WLY-B-L8T likely represents a separate species in the genus _Bacillus_. \begin{table}
\begin{tabular}{c c c c c} \hline
**Characteristics** & **1** & **2** & **3** & **4** \\ \hline D-Xylose & \(-\) & \(-\) & \(-\) & \(-\) \\ D-Galactose & \(-\) & \(-\) & \(-\) & \(-\) \\ D-Glucose & \(+\) & \(+\) & \(+\) & \(+\) \\ D-Fructose & \(+\) & \(+\) & \(+\) & \(+\) \\ D-Mannose & \(-\) & \(-\) & \(-\) & \(+\) \\ D-maltose & \(+\) & \(+\) & \(+\) & \(+\) \\ \(N\)-Acetylglucosamine & \(+\) & \(+\) & \(+\) & \(+\) \\ Arbutin & \(-\) & \(-\) & \(+\) & \(+\) \\ Aesculin ferric citrate & \(-\) & \(-\) & \(+\) & \(+\) \\ Salicin & \(+\)w & \(-\) & \(+\) & \(+\) \\ Cellobiose & \(-\) & \(+\) & \(+\) & \(+\) \\ Sucrose & \(+\) & \(-\) & \(+\) & \(-\) \\ Trehalose & \(-\) & \(-\) & \(+\) & \(-\) \\ Starch & \(+\) & \(+\) & \(+\) & \(-\) \\ Glycogen & \(-\) & \(+\) & \(+\) & \(-\) \\ Potassium gluconate & \(-\) & \(-\) & \(-\) & \(-\) \\ DNA G+C content (mol\%) & 35.97 & 36.39 & 35.37 & 35.87 \\ \hline \end{tabular} Strains: 1, WLY-B-L8T; 2, _Bacillus rhizolanue_ JJ-63 DSM 12442T; 3, _Bacillus pseudomycoides_ DSM 12442T; and 4, _Bacillus cytotoxic_ NVH 391–98T. Data for strains 2–4 from [(24)]. In the API 20E tests, all strains were negative for lysine decarboxylase, drinking decarboxylase, H\({}_{2}\)S production, tryptophan deaminase indole production, mannitol, inositol,orhombic, mannose, sucrose, melibulose, amygdala, and arabinose. In the API 50 CHB tests, all strains were negative for erythroid, D-arabinose, L-arabinose, L-xylose, D-adonitol, methyl \(\beta\)-D-xylopyranoside, L-sorbedse, L-hamnose, dalcitol, inositol, mannitol, sorbitol, methyl \(\alpha\)-D-mannopyranoside, D-lactose, D-melibiose, inulin, D-mezielose, D-farnose, xylitol, D-gentobiose, D-lucrose, D-taco, D-tacose, L-rabitol, L-arabitol, potassium 2-ketogluconate, and potassium 5-ketogluconate. +, positive; \(-\), negative; +w, weakly positive; NR, not reported, the parameter was not tested.  
\end{table} 
Table 1: _Cont._