## 3 Results and Discussion
### HPLC Analysis  
Extracts of St. John's word were analyzed qualitatively and quantitatively using HPLC chromatography. The GL-WE and PEG-WE contain chlorogenic acids, _p_-coumaric acid, rutin, hyperoside, and isoquercitrin (Table 4); their chromatograms are shown in Figure 1. The differences in the composition of both extracts are noticeable. GL-WE contains more rutin, hyperoside, and isoquercitrin than PEG-WE, while the latter contains more chlorogenic acids. All mean values differ statistically significantly (_p_\(\leq\) 0.05), except for the _p_-coumaric acid concentration. There are no data in the literature regarding differences in the extraction efficiency of polyphenols for glycol and glycerol. [PERSON] et al. [52] investigated the dependence of \begin{table}
\begin{tabular}{c c c c} \hline \hline \multirow{2}{*}{**H. _perforatum_ Extract**} & **GL-WE** & **PEG-WE** \\  & **[mg/100 mL \(\pm\) SD]** & **[mg/100 mL \(\pm\) SD]** \\ \hline
1 & nCGA & 10.37 \(\pm\) 0.04 a  & 16.36 \(\pm\) 0.05 b  \\ \hline
2 & _p_-Coumaric acid & 3.71 \(\pm\) 0.01 a  & 2.09 \(\pm\) 0.04 a  \\ \hline
3 & CGA & 7.28 \(\pm\) 0.04 a  & 10.94 \(\pm\) 0.07 b  \\ \hline
4 & Rutin & 40.65 \(\pm\) 0.19 a  & 7.12 \(\pm\) 0.09 b  \\ \hline
5 & Hyperoside & 23.26 \(\pm\) 0.12 a  & 9.01 \(\pm\) 0.12 b  \\ \hline
6 & Isoquercitrin & 10.47 \(\pm\) 0.09 a  & 1.65 \(\pm\) 0.05 b  \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Content of phenolic acids and flavonoids in _H. perforatum_ extracts. Figure 1: HPLC chromatograms of GL-WE (**a**) and PEG-WE (**b**) extracts registered at 330 nm. 1—neochlorogenic acid; 2—_p_-coumaric acid; 3—chlorogenic acid, 4—rutin; 5—hyperoside; 6—isoquercitrin. the total content of polyphenols, flavonoids, and antioxidants, as well as the scavenging of free radicals by DPPH and the concentration of flavonoid aglycones in the extract of _Chamanerion angustiifolium_ L., on the type used for the extraction of NADES containing glycerol and propylene glycol. The highest yield of polyphenols was obtained using NADES containing glycerol; however, an important factor in this case was the type of hydrogen bond acceptor and temperature. Classical liquid extraction using a water/glycerol mixture showed a high yield of polyphenols (chlorogenic acids and quercetin glycosides). In the GL-WE model examined, these substances were also predominant. Owing to the use of fatty oils containing medium-chain triglycerides as extractants, quercetin, kaempfer, biapigenin, hyperforin, and adhyperforin were extracted from the raw material [53]. Chromatographic analysis in our study did not show the presence of naphthodi-anthrones in the tested extracts, probably due to limited solubility in almost all solvents. Currently, according to the Polish Pharmacopoeia X, the raw material is standardized on the total content of hypericin, which must be no less than 0.08% [54]. The total content of hypericin depends on the latitude, time of harvest, plant's habitat, drying and storage methods, and other factors [55; 56]. Hypericin is a hydrophobic substance that is poorly soluble In water and oil; however, it is highly soluble in some solvents, such as ethanol, methanol, and ethyl acetate [57]. Nevertheless, some studies have identified hypericin in aqueous extracts [58; 59]. Pseudohypericin is more soluble in polar solvents, which is mainly attributed to the presence of an additional hydroxymethyl moiety [60]. The use of hypericin in cosmetics is made difficult by its phototoxicity, poor water solubility, and high light sensitivity. In the GL- and PEG-based extracts examined in this study, the presence of naphthodianthrones, including hypericin, was not detected, which is advantageous for cosmetic applications. Moreover, hyperoside, a compound characteristic of _H. perforatum_, was present in predominant amounts alongside ruitin in the GL-WE extract studied. In the study by [PERSON] et al. [61] it was shown that hyperoside acts as an effective protective compound against UV radiation.