## 3 Results
### Changes in General Skin Features  
Erythema, hemorrhage, scarring, and erosion were observed in the LBE group. In contrast, PEAT and 3 HbT groups showed comparatively less skin damage (Figure 3A). Histochemical changes in the skin were observed after hematoxylin and eosin staining. In LBE group, epithelial cell formation, expansion of intercellular space in the spinous layer, increased lymphocyte erosion, and decay of the basal layer were observed in the stratum \begin{table}
\begin{tabular}{c c c c c c c c c c c} \hline \hline
**Sample** & \multicolumn{8}{c}{**Chromatopable Results (mg/kg) [FOOTNOTE:]Footnote : Glycyrrhizia: _Rizione_ of _Glycyrrhizia unless_; [ENDFOOTNOTE] } \\
**Information** & **Liquiritin** & **Liquiritingenin** & **Berberine** & **Palmatine** & **Daidzein** & **Glycticin** & **Genistein** & **Daidzin** & **Glycitin** & **Geinistin** \\ \hline Glycyrrhiziae (1) & 3.51 & 0.10 & - & - & - & - & - & - & - & - \\ Coptis (2) & - & - & 4.84 & 14.88 & - & - & - & - & - & - \\ Glycine (3) & - & - & - & - & 0.03 & 0.01 & 0.02 & 0.17 & 0.09 & 0.33 \\
3 Hb (1:1) & 0.25 & 0.49 & 7.19 & 21.97 & 2.96 & 0.55 & 0.06 & 2.19 & 1.13 & 0.25 \\ \hline \hline \end{tabular}  
* Glycyrrhizia: _Rizione_ of _Glycyrrhizia unless_; [ENDFOOTNOTE]
* COpts: _Rizione_ of _Coptis chinensis_; [ENDFOOTNOTE]
* Glycine: _Semen_ of _Glycine max_; [ENDFOOTNOTE]
* Each standard compound was present in the free form.  
\end{table} 
Table 1: Contents (mg/kg) of the markers of processed herbal medicine extracts evaluated by high-performance liquid chromatography. Figure 2: Effect of Three Herb (3 Hb) extract on viability and on expression of filaggrin in HaCaT cells. 3 Hb means _Rhizione_ of _Coptis chinensis_, _Rhizome_ of _Glycyrrhizia__valensis_, and fermented _Semen_ of _Glycine max_. Cells were treated with the indicated concentration of 3 Hb for 24 h. HaCaT, Human keratinocytes. The data were analyzed using a Student’s unpaired _t_-test. _p_\(<\) 0.05 were considered significant. * _p_\(<\) 0.05 compared to the untreated (0 ug/mL) group. basale. In contrast, the epithelia of PEAT and 3 HbT groups showed comparatively less damage (Figure 3A). The clinical skin scores for each group are shown in Figure 3B. The clinical skin scores of 3 HbT mice were lower than those of PEAT and LBE groups (\(p<0.01\)). The pH values of each group are shown in Figure 3C. FLG deficiency results in an increase in the pH of the SC due to the reduction of trans-urocanic acid (UCA) [26]. Therefore, the pH of the dorsal skin of the LBE group was higher than that of the dorsal skin of the Ctrl group. The pH values of the dorsal skin of the PEAT and 3 HbT groups were lower than those observed in the LBE group (\(p<0.05\)). Furthermore, the pH values of 3 HbT mice were lower than that of PEAT group (\(p<0.05\)). The extent of TEWL in each group is shown in Figure 3D. FLG deficiency is known to weaken the formation of keratinous cells and reduce adhesion between keratinous cells, resulting in an increase in TEWL [26]. Therefore, the TEWL in LBE group was higher than that in the Ctrl group. Moreover, TEWL in the 3 HbT group was lower than that in the PEAT group (\(p<0.05\)). These results indicate that lipid barrier elimination causes inflammation in the skin, causing conditions such as AD and an increase in TEWL and pH of the skin. The 3 Hb extract effectively alleviated inflammation in the skin and decreased TEWL and pH in the skin compared to PEA. Figure 3: The alleviation of symptoms of lipid barrier elimination using Three Herb (3 Hb) extract. **(A)** The alleviation of symptoms of lipid barrier elimination. The lipid destruction-induced skin lesions were relieved in 3 HbT compared to PEAT and LBE. Clinical skin score **(B)**, pH **(C)** and TEWL **(D)** was significantly lower in 3 HbT compared with PEAT and LBE (Immunohistochemistry; bar size, 50 \(\upmu\)m).