# Characteristic of _Pseudomonas syringae_ pv. _atrofaciens_ Isolated from Weeds of Wheat Field
## 3 Results
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Strain** & **Host Plant** & **Source** & \begin{tabular}{c} **Year of** \\ **Isolation** \\ \end{tabular} & \begin{tabular}{c} **Sero-** \\ **Group** \\ \end{tabular} & \begin{tabular}{c} **Aggressiveness** \\ **for Wheat** \\ \end{tabular} & **for Weeds** \\ \hline L8 & _Papaver argemone_ L. & \begin{tabular}{c} wheat field, \\ Kyiv region \\ \end{tabular} & 2019 & 2 & 2 & 1 \({}^{*}\) \\ \hline L9 & _Papaver argemone_ L. & \begin{tabular}{c} wheat field, \\ Kyiv region \\ \end{tabular} & 2019 & 2 & 2 & 1 \({}^{*}\) \\ \hline L11 & _Sonchus arensis_ L. & wheat field, Kyiv region & 2019 & 2 & 1 & 2 \({}^{**}\) \\ \hline L11a & _Sonchus arensis_ L. & wheat field, Kyiv region & 2019 & 2 & 2 & 2 \({}^{**}\) \\ \hline L13 & _Sonchus arensis_ L. & wheat field, Kyiv region & 2019 & 6 & 2 & 2 \({}^{**}\) \\ \hline L15 & _Sonchus arensis_ L. & wheat field, Kyiv region & 2019 & 6 & 3 & 2 \({}^{**}\) \\ \hline L16 & _Sonchus arensis_ L. & wheat field, Kyiv region & 2019 & 6 & 3 & 3 \({}^{**}\) \\ \hline \multicolumn{5}{c}{_P. syringae_ pv. _atrofaciens_ 9780} \\ \hline \multicolumn{5}{c}{_P. syringae_ pv. _atrofaciens_ UCM & \begin{tabular}{c} **Tritcium aestivum_** \\ \end{tabular} \_L. & \begin{tabular}{c} Collection of \\ microorganisms \\ of the Department of \\ phytopathogenicic bacteria \\ \end{tabular} & 1972 & 2 & 4 & 2 \({}^{**}\) \\ \hline \multicolumn{5}{c}{_P. syringae_ pv. _atrofaciens_ UCM & \begin{tabular}{c} **Tritcium** sp. \\ \end{tabular} & \begin{tabular}{c} **Tritcium** sp. \\ \end{tabular} & \begin{tabular}{c} **PDCCC 4394** \\ \end{tabular} & 4 & 4 & N/D \\ \multicolumn{5}{c}{B-1011} \\ \hline \multicolumn{5}{c}{_P. syringae_ pv. _syrinage_ UCM & \
\begin{tabular}{c} _Syrinage vulgaris_ L. \\ \end{tabular} &
\begin{tabular}{c} NCPPB 281 \\ \end{tabular} & 1 & 4 & N/D \\ \multicolumn{5}{c}{B-1027} \\ \hline \hline \end{tabular}  
\({}^{*}\)—aggressiveness for _P. argemone, \({}^{**}\)_—aggressiveness for _S. arrensis_, N/D—no data.  
\end{table} 
Table 2: Virulence and serological properties of the bacterial strains used in this study. (L8, L9) were belonged to serogroup II, while strains isolated from _S. arvensis_ assigned to serogroup II (L11, L11a) and serogroup VI (L13, L15, L16) (Table 2). When studying the composition of fatty acids of total cellular lipids of the isolates L8, L9, L11, L11a, L13, L15, L16, the presence of the following fatty acids was detected: saturated--dodecanoic (C12:0), tetradecanoic (C14:0), hexadecanoic (C16:0), octadecanoic (C18:0); unsaturated--cis-9-hexadecene (C16:1), cis-11-octadecene (C18:1); cyclic--cis-9,10-methylenehexadecanoic (C17:0 cyclo), cis-9,10-methylenoctadecanoic (C19:0 cyclo), hydroxy acids--3-hydroxydecanoic (3-OH C10:0), 2-hydroxydodecanoic (2-OH C12:0), and 3-hydroxydodecanoic (3-OH C12:0) (Figure 2). There were three predominant fatty acids: C16:0, C16:1, and C18:1. The last ones' content was 25.67-28.89%, 41.63-47.98%, and 12.13-17.12% in the cells of newly isolated strains of _P. syringae_. This was similar to the content of predominant fatty acids in the cells of collection strains of _P. syringae_: 28.28-34.25%, 31.16-38.78%, and 18.25-24.49%, respectively. Besides, hydroxy acids have an important role in the taxonomy of _P. syringae_. Three hydroxy fatty acids were identified in all investigated strains from 0.24 to 4.50%. The fatty acid profiles of all seven isolates were similar to each other and the collection strains of the causative agent of the basal glume rot of wheat, isolated from the infected wheat plants. We carried out a comparative analysis of the fatty acid composition of the strains isolated from weeds and _P. syringae_ pv. _atrofaciens_ strains, isolated from wheat (2399, 9400, 9417, 9748, 9819, 9771, 9785, P204, and P203) and isolated from rye (7194, 7836, 7959, 8099, 8116, 8274, 8281, 8317, 8462, 8904, and 9010). All these strains were described by us earlier in the works [22,23].