## 2 Materials and Methods
### Identification and Biochemical Characteristics of YHM-G  
The target yeast strain YHM-G was identified by morphological and physiological characteristics and ribosomal large subunit 265 rRNA gene D1/D2 domain sequence, as described by [PERSON] et al. [20]. YHM-G was examined for colony morphology on the WL plate by the naked eye and cell morphology using a microscope (Nikon, Tokyo, Japan), respectively. Carbon and nitrogen source assimilation and sugar fermentation tests were conducted in stationary cultures as a sole carbon or nitrogen source. Four slanted milliliters of urea medium, which were placed in slants, were applied to the incubated yeast colony at room temperature. Red-pink color in the medium was considered to be a positive test for urea test. For the indole test, the appearance of bright red and yellow color composed after adding 0.5 mL of [PERSON]'s reagent to incubated culture at 30 \({}^{\circ}\)C for 24 h on indole test media indicated positive and negative results, respectively. After adding methyl red indicator solution to inoculated culturing media and incubating at 30 \({}^{\circ}\)C for up to 7 days, the changing color to red indicates that the methyl red test is positive. For the Voges-Proskauer test after incubation of 48 h Voges-Proskauer Reagent A (5% naphthol) and Voges-Proskauer Reagent B (40% KOH) were added and a change in color was observed. YHM-G was inoculated on triple sugar iron agar slant and cultured at 30 \({}^{\circ}\)C for 48 h, and results were determined based on color change. Citrate test was performed via inculating Simmons Citrate Agar plates surface with yeast cultures, then incubating at 30 \({}^{\circ}\)C up to 48 h, and the media color changing from green to bright blue indicates a positive reaction. A gelatin liquidized test was applied; when YHM-G was inoculated into tubes containing gelatin, gelatin liquefaction was the positive result. To confirm the starch hydrolysis activity, YHM-G was grown on nutrient agar containing 1% soluble starch and incubated at 30 \({}^{\circ}\)C for 72 h. Hydrolysis was observed as the formation of a colorless zone around the colony after flooding the plate with Gram's iodine. The ribosomal large subunit 26S rRNA gene D1/D2 domain fragment from strain YHM-G was amplified using universal primers (NL1: 5'-GCATATCAATAAAGCGGAGGAAAAG-3' and NL4: 5'-GGTCCGTGTTTCAAGACG-3'), as described by [PERSON] and [PERSON] (2011). Then, the resulting sequence was submitted to the NCBI database ([[https://www.ncbi.nlm.nih.gov/](https://www.ncbi.nlm.nih.gov/)]([https://www.ncbi.nlm.nih.gov/](https://www.ncbi.nlm.nih.gov/)), accessed on 17 July 2022) with accession number ON999037 and a BLAST search was conducted against the Nucleotide collection (nr/nt) database using the BLASTN program. All reference sequences were from type or otherwise authenticated strains. Moreover, the overlapping fragment of D1/D2 domain of the ribosomal large subunit 26S rRNA gene was then aligned using ClustalW. The sequence alignment was filtered in ARB to exclude gaps, missing data, ambiguous nucleotide positions and lowercase positions. A phylogenetic tree was constructed by the neighbor-joining method (MEGA version 7 software) using the Kimura 2-parameter model ([PERSON], 2012) and tested by bootstrap with 1000 repetitions ([PERSON], 2012).  
The growth response was measured in a certain range of parameters, including temperature (20, 25, 30, 35, 40, 45 and 50 \({}^{\circ}\)C), pH (1-14), glucose concentration (23, 29, 33, 38, 41, 44 and 47%, _w/w_), NaCl concentration (0, 3, 6, 8, 11, 13, 15, 17, 19 and 21%, _w/w_), ethanol concentration (0, 3, 6, 9 12, 15, 18 and 21%, _v/v_) and 3-Met concentration (5, 10, 20, 30, 35, 40, 45 and 50 g/L) by monitoring the optical density at 560 nm (OD\({}_{560}\)) of the YPD medium, as reported previously ([PERSON], 2012).  
The aroma compounds produced by YHM-G in SHM were carried out as described previously ([PERSON], 2012).