## 4 Discussion
### Effect of Moisture Content and Additives on the Fermentation Quality of AWB Mixed Silage
[43] showed that the addition of cellulase increased the ME and CP contents and decreased the NDF and ADF contents in king grass silage, which was consistent with the above experimental results found in this study. [PERSON] et al. [38] found that the combined addition of lactic acid bacteria and cellulase reduced the ADF content in _Leymus chinensis_ silage. In _Pennisetum sinese_ silage, cellulase reduced the crystallinity of lignocellulosic and improved its conversion, indicating that it was a very useful silage pretreatment method [44]. Under 60% MC conditions, the NDF, ADF, and ADL contents in the L group were lower than those in the control group, which was consistent with the findings of [PERSON] et al. [31]. These authors also found that inoculation of lactic acid bacteria in _Pleurotus eryngii_ silage reduced the NDF, ADF, and ADL contents in _P. eryngii_ silage. This result was surprising because the lactic acid bacteria silage treatment lacked the relevant cellulose-degrading enzymes and could not substantially affect the structure of the lignin-cellulose-hemicellulose complex [45]. This was explained by the fact that the addition of lactic acid bacteria preparations to silage produced more lactic acid, which acidified the cell wall and led to lower NDF and ADF contents. Under the three MC conditions, the DM content in the L, E, and M groups was lower than that in the control group. [PERSON] et al. [46] showed that the DM content could be depleted by 2% in the presence of lactic acid bacteria or cellulase, which was consistent with the above experimental results found in this study. Nutrient digestibility is one of the important factors affecting feed intake [47]. Under 60%, 65%, and 70% MC, the IVDMD, IVCPD, IVOMD, and IVNDFD contents in AWB mixed silage were higher in the E and M groups than in the control group. The nutrient digestibility of AWB mixed silage was enhanced due to the superimposed effect of the enzymatic interaction between the rumen microbiota and exogenously added cellulase [48]. [PERSON] et al. [10] supported these findings regarding increased IVDMD after exogenous addition of cellulase. ADF and NDF are the most effective indicators that reflect the quality of fiber at this stage. The ADF content is negatively correlated with animal digestibility; the lower its value is, the higher the digestibility of the feed and the greater the feeding value. [PERSON] et al. [49] added an enzyme preparation containing cellulase to buffalo calf diets and found that calves fed the enzyme preparation had significantly higher IVDMD, IVCPD, and IVNDFD than the control group, which was consistent with the results in this study for trials in which E and M were added at 60%, 65%, and 70% MC. The total calf weight gain and average daily weight gain were also significantly higher than those in the control group, indicating that the addition of the enzyme preparation improved the productive performance and nutrient digestibility of the animals. This may be because cellulose and its derivatives in silage can be catalytically degraded by cellulase preparations, breaking the cell wall barrier composed of cellulose, pectin, hemicellulose, and lignin together, and the nutrients in the cell wall can be released, which then interact with digestive enzymes to improve feed digestibility and animal growth performance. According to [PERSON] et al. [50], lactic acid bacteria can survive in rumen fluid, suggesting that lactic acid bacteria may directly influence rumen fermentation in a way that enhances rumen microbial biomass. Alfalfa silage inoculated with lactic acid bacteria produced more microbial biomass and altered rumen fermentation compared to a control group, which may positively affect the productive performance of ruminants. This change in rumen fermentation may be associated with better preservation of feed proteins during silage fermentation [51]. At 60% MC, the L treatment increased the IVDMD and IVCPD of the AWB mixed silage compared to the control.