# Identification and Quantification of Valuable Compounds in Red Grape Seeds
## 2 Materials and Methods  
### Materials  
Seven varieties of red grapes were selected for this study, namely Cabernet Sauvignon, Merlot, Pinot noir, Burgund Mare, Cadarca, Syrah, Novac from the hilly area of Banat. Cabernet Sauvignon is a red grape variety with the aroma of berries that have a thick and weather-resistant skinand the wine obtained has a high tannin content [38]. Following several studies and HPLC analysis, it was found that the Cabernet Sauvignon grape variety has a high content of resveratrol (52.3 and 49.6 mg/kgDW) [39]. Merlot is the second most planted variety in the world after Cabernet Sauvignon. The variety adapts to different microclimates, resistant to colder temperatures, in such conditions, it expresses itself better [40]. In the wine obtained from the Merlot grape variety, there is a high content of glycerol, ethyl acetate and succinic acid. These are giving the wine a specific taste and a well-pronounced flavor [41]. The grapes of the Pinot noir variety have thin skin and do not load the grape must. Pinot noir wines are always transparent. This variety likes cooler areas where it draws its freshness and subtle fruitiness that does not overwhelm other flavors [42]. Pinot noir is considered to be a difficult variety of product, being sensitive to the environment, with thin skin, has a high susceptibility to disease and lower concentrations of anthocyanin that often lead to reduced intensity of wine color [43]. The Burgund Mare variety has existed in Romania for over a century, but it has expanded considerably in culture after 1975 and offers high grape production. The wine does not have a specific personality, but it is balanced, with extractive and medium color intensity, reaching an alcoholic strength of 11-12 vol% [44]. \"Cadarca\" or \"Cadarca de Minis\" is a traditional grape variety originating from the Minis-Maderat vineyards, Arad area, where it was first prepared in 1744. The wine obtained is called from ancient times \"bull's blood\", a reputation that the locals keep even today, defining the red-ruby color, personality and strength. The grapes are medium-sized, cylindrical, sometimes winged, with the berries often placed in clusters and uneven in size. The bean is medium, spherical, slightly ovoid, with thin skin and unevenly colored in bluish-black. The variety is resistant to frost but has low resistance to drought, summer rains in sunny weather with a detrimental effect on plant development and medium resistance to grey rot of grapes [45]. Syrah is a red grape variety from France, from the Rohne Valley. Being a traditional French wine, Syrah expresses floral aromas in its youth, and over time, it develops aromas of black and white pepper, grassy notes, skin and smoke [46]. Novac is a grape variety with red grapes for red wines. It was obtained at SCDVV Dragasani by sexual hybridization between the varieties Negru vartos and Saperavi, being approved in 1987. It has medium-sized grains, weighing in the range of 168-315 g/100 grains, ovoid in shape, with black-bluish skin, covered with plum. The flesh is red, juicy, with a pleasant sweet and sour aroma and taste. The main climatic data from the test period were represented by the average temperature, with values between 11.4 and 12.5 \({}^{\circ}\)C [47]. Grapes were crushed by hand, the seeds separated by pulp and shell. The seeds were then washed under running water and then dried at room temperature. After drying, they were ground. The powder obtained was subjected to extraction with petroleum ether for 8 h. For each seed sample, 20 g of product was placed in an extraction cartridge and so on degreased, applying the Soxhlet method at a temperature of 50 \({}^{\circ}\)C. The samples were then homogenized with 50 mL solvent consisting of nine parts acetone and one part distilled water for the extraction of polyphenols for 12 h. Subsequently, they were centrifuged for 30 min at 3500 revolutions/min, filtered and evaporated in rotavapor--1:10.