# Identification of Blackberry (_Rubus fritticosus_) Volatiles as _Drosophila suzukii_ Attractants
## Results
### Identification and Quantification of R. fruticosus Volatiles
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Compound** & **RT** & **RI** & **Log(Peak Area)** & \
\begin{tabular}{c} **Volume Present in** \\ **1 Berry (\(\mu\)L)** \\ \end{tabular} &
\begin{tabular}{c} **Volume Used in** \\ **Bioassay (\(\mu\)L)** \\ \end{tabular} \\ \hline Acetaldehyde & 0.93 & 408 & - & - & 0.2 \\ \hline Ethanol & 1.02 & 446 & - & - & 0.2 \\ \hline Toluene & 4.95 & 759 & 6.41 & \(8.59\times 10^{-6}\) & 0.00009 \\ \hline Hexanal & 5.90 & 793 & 6.85 & \(7.94\times 10^{-5}\) & 0.0008 \\ \hline (E)-2-Hexenal & 7.46 & 849 & 7.58 & \(2.35\times 10^{-4}\) & 0.002 \\ \hline (Z)-3-Hexen-1-ol & 7.65 & 856 & 7.13 & \(7.93\times 10^{-5}\) & 0.0008 \\ \hline (E)-2-Hexen-1-ol & 7.99 & 868 & 8.22 & \(1.11\times 10^{-3}\) & 0.01 \\ \hline
1-Hexanol & 8.09 & 872 & 8.36 & \(1.15\times 10^{-3}\) & 0.01 \\ \hline
2-Heptanone \({}^{\star}\) & 8.55 & 888 & 7.32 & \(1.21\times 10^{-4}\) & 0.001 \\ \hline
2-Heptanol & 8.93 & 902 & 8.39 & \(1.87\times 10^{-3}\) & 0.02 \\ \hline beta-Pinene & 9.50 & 919 & 4.9 & - & - \\ \hline alpha-Pinene & 9.69 & 932 & 7.41 & \(4.35\times 10^{-5}\) & 0.0004 \\ \hline Camphene & 10.09 & 943 & 7.12 & \(2.63\times 10^{-5}\) & 0.0003 \\ \hline Thuja-2,4(10)-diene & 10.28 & 950 & 6.4 & - & - \\ \hline beta-Myrcene & 11.38 & 987 & 6.52 & \(1.11\times 10^{-5}\) & 0.0001 \\ \hline Hexyl acetate & 12.05 & 1009 & 6.74 & \(1.78\times 10^{-5}\) & 0.0002 \\ \hline (E)-2-Hexen-1-ol, acetate & 12.13 & 1016 & 6.39 & \(1.74\times 10^{-5}\) & 0.0002 \\ \hline para-Cymene & 12.40 & 1025 & 7.26 & \(3.60\times 10^{-5}\) & 0.0004 \\ \hline L-Limonene & 12.53 & 1028 & 7.83 & \(1.69\times 10^{-4}\) & 0.002 \\ \hline beta-Ocimene & 13.19 & 1044 & 6.33 & \(1.05\times 10^{-5}\) & 0.0001 \\ \hline gamma-Terpinene & 13.56 & 1055 & 6.91 & \(2.24\times 10^{-5}\) & 0.0002 \\ \hline
1-Octanol \({}^{\star}\) & 14.13 & 1071 & 6.02 & \(5.06\times 10^{-6}\) & 0.00005 \\ \hline Terpinolene & 14.63 & 1084 & 7.55 & \(7.35\times 10^{-5}\) & 0.0007 \\ \hline p-Cymenene & 14.74 & 1088 & 7.75 & \(7.55\times 10^{-5}\) & 0.0008 \\ \hline Linalool \({}^{\star}\) & 15.10 & 1101 & 6.12 & \(6.29\times 10^{-6}\) & 0.00006 \\ \hline
1,3,8-p-Menthatriene & 15.61 & 1110 & 5.84 & - & - \\ \hline Nonanal & 15.70 & 1115 & 6.35 & \(6.72\times 10^{-6}\) & 0.00007 \\ \hline trans-para-Mentha-2,8-dien-1-ol \({}^{\star}\) & 16.03 & 1120 & 6.49 & - & - \\ \hline (E,E)-Cosmene & 16.63 & 1134 & 6.68 & - & - \\ \hline Camphor & 16.92 & 1141 & 6.32 & \(2.42\times 10^{-6}\) & 0.00002 \\ \hline cis-Verbenol & 17.10 & 1145 & 6.35 & \(5.83\times 10^{-5}\) & 0.0006 \\ \hline Borneol \({}^{\star}\) & 17.98 & 1164 & 7.19 & \(4.07\times 10^{-5}\) & 0.0004 \\ \hline Terpinen-4-ol & 18.54 & 1176 & 7.69 & \(1.84\times 10^{-2}\) & 0.2 \\ \hline p-Cymen-8-ol \({}^{\star}\) & 18.89 & 1183 & 7.05 & \(5.84\times 10^{-5}\) & 0.0006 \\ \hline Hexyl butanoate \({}^{\star}\) & 18.99 & 1191 & 7.1 & \(2.37\times 10^{-3}\) & 0.02 \\ \hline Myrtenal & 19.36 & 1192 & 6.86 & \(8.07\times 10^{-6}\) & 0.00008 \\ \hline Myrtenol \({}^{\star}\) & 19.53 & 1196 & 7.06 & \(2.03\times 10^{-2}\) & 0.2 \\ \hline Decanal \({}^{\star}\) & 19.73 & 1205 & 6.83 & \(1.57\times 10^{-3}\) & 0.02 \\ \hline \hline \end{tabular}
\end{table} 
Table 1: List of all the volatile compounds identified and quantified via multiple headspace SPME GC-MS in _Rubus fruticosus_. The retention time (RT) and calculated retention index (RI) of each compound is shown. Summed peak area over fifteen repeated headspace extractions were calculated from the geometric mean sample average (\(n=4\) samples). Compounds that show no calculated volume were not commercially available or were too volatile to be quantified accurately (acetaldehyde and ethanol). Asterisks indicate compounds with no clear reduction in peak area over fifteen extractions.