## 2 Materials and Methods
### Animals  
Thirty bulls (10 bulls per evaluated system) were reared in good-quality pasture until they were seven months old with mothers. Then, the bulls were reared up to 350.0 kg on corrected pasture and with multiple forage species (tyfton, clover, alfalfa, chicory, oats and peas), eight months, from 350.0 kg to 542.3 kg of body weight. In this system, the bulls were reared in feedlot receiving a diet based on this same forage, but in ensiled form (five months). These animals were finished in the feedlot system from 450.0 kg until slaughter at 580.0 kg.  
Industrial crossbreeding between the Angus and Nellore breeds born in August 2022, raised and reared in an extensive pasture system and finished in a feedlot system were used. The animals were chosen by their weight at weaning time (seven months).  
All animals were vaccinated against symptomatic anthrax, gas gangrene, sudden death, enterotoxemia, malignant edema, tetanus and botulism (Excell 10) and pneumonia (Bovic Shield). Likewise, the animals were dewormed every six months (solution 3.5% (MSD) + genisis iver puro on (genesis), solution 3.5% (MSD) + agebendazol + volosso (Ouro Fino), tree ace (Zoetis) + agebendazol + colosso (Ouro Fino). The stocking rate was three animals/hectare.  
The average daily gain (ADG) was monitored periodically. The average daily gain (ADG) was obtained by dividing the final weight or slaughter weight by the number of days until slaughter. No bull had to be removed from the experiment, due to failure to adapt or for other reasons.  
At the end of the finishing period, the bulls were weighed and transported to the slaughterhouse in the city of Campo Mourao, North of Parana, 60 km away, after a period of 18 h of fasting from solids, with water always being available. The density of the truck was \(0.8+0.2\) m\({}^{2}\)/animal.  
The slaughter was carried out at the Magistral slaughterhouse, in the city of Campo Mourao, state of Parana, as recommended by the Brazilian system according to RIISPOA (Regulation for the Industrial and Sanitary Inspection of Products of Animal Origin) [29].  
The bulls were stunned with a compressed air gun. They were then bled, with a suitable knife cut through the external jugular vein and the hide, head, feet, tail, diaphragm and pelvic fat (kidney and heart) removed.  
\begin{table}
\begin{tabular}{l c c c} \hline
**Nutriments** & **Pasture 1** & **Pasture 2** & **Feedlot** \\ \hline Dry matter, \% & 14.10 & 57.38 & 86.18 \\ Crude protein, \%/DM & 10.87 & 12.52 & 14.57 \\ Organic matter, \%/DM & 85.13 & 86.72 & 91.06 \\ Ashes, \%/DM & 14.87 & 13.28 & 8.94 \\ Ether extract, \%/DM & 2.88 & 2.26 & 4.42 \\ Crude fiber, \%/DM & 32.00 & 20.57 & 11.89 \\ Neutral detergent fiber, \%/DM & 63.05 & 38.65 & 25.23 \\ Acid detergent fiber, \%/DM & 42.56 & 24.53 & 15.01 \\ Lignin, \%/DM & 12.68 & 4.22 & 3.00 \\ Starch, \%/DM & 4.58 & 21.51 & 3.60 \\ Carbohydrates non fibers, \%/DM & 11.25 & 34.42 & 48.56 \\ Total digestible nutrients, \%/DM & 45.61 & 68.65 & _77.77_ \\ Metabolizable energy, Kcal/kg & 2.150 & 2.610 & 3.000 \\ \hline \end{tabular} 1 Low Quality Pasture (_Brachiaria raizieneics_). 2 Good Quality Pasture (_Brachiaria_ pasture, fertilized and corrected).  
\end{table}
Table 1: Chemical composition of experimental diets.