## 4 Discussion  
The main observation of the in vivo experiment is a disruption of CO\({}_{2}\) elimination from the animals' lungs, which affects the internal environment of the animals' organism using an orifice plate. Compensation for the pressure loss caused by the used differential orifice plate is possible by increasing the PIP inspiratory pressure on the Life Pulse ventilator, but only in case of external monitoring of chest excursions. A hypothesis determined before the experiment indicates that a negative impact on the animal model's physiological parameters using a pressure orifice plate (flow sensor) at high-frequency jet ventilation is expected. This assumption is given mainly because the phenomenon of permanent pressure loss in these flow sensors is known in the literature [27????] and confirmed by the work [27??]. On the other hand, the described experiments showed that all observed negative manifestations (decrease in pressure amplitude in the lung model and deterioration of CO\({}_{2}\) elimination) could be compensated for by increasing the jet breath's pressure amplitude using the PIP parameter. Based on these findings, PaCO\({}_{2}\) and pH parameters were monitored in animal models, and CO\({}_{2}\) was expected to increase and pH to decrease (??????). The PaO\({}_{2}\) parameter was also monitored as the oxygenation indicator of the animal models. Since the same fraction of oxygen in the ventilation mixture was not always used in experiments, the oxygenation index was used for the determination of the oxygenation, and to see the relationship (1), taking into account not only SiO\({}_{2}\) but also the mean pressure in the airways, measured by the LifePulse ventilator. Boxplots express the graphic illustration of the results in??????. The Friedman test (results in Table??) confirmed changes in PaCO\({}_{2}\) and pH values at respiratory rates of \begin{table}
\begin{tabular}{l c c c c c} \hline \hline  & \multicolumn{2}{c}{**Physiological Parameter**} & \multicolumn{2}{c}{**Friedman’s ANOVA**} & \multicolumn{2}{c}{**Post-Hoc Analysis**} \\ \cline{3-6}  & & **None–Sens** & **None–SensComp** & **Sens–SensComp** \\ \hline RR 240 & pH & 0.0075 & 0.0117 & 0.2076 & 0.0251 \\  & PaCO\({}_{2}\) & 0.0022 & 0.0128 & 0.6744 & 0.0118 \\  & OI & 0.6873 & & Do not reject the null hypothesis \\ \hline RR 420 & pH & 0.0137 & 0.0117 & 0.0279 & 0.0499 \\  & PaCO\({}_{2}\) & 0.0046 & 0.0117 & 0.1834 & 0.0208 \\  & OI & 0.0207 & 0.0923 & 0.0117 & 0.2626 \\ \hline RR 660 & pH & 0.0724 & & & \\  & PaCO\({}_{2}\) & 0.1353 & & Do not reject the null hypothesis \\  & OI & 0.8825 & & & \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Results of [PERSON] test and post-hoc analysis. Figure 11: Graphic illustration of monitored parameters of rabbits (\(n=8\)) at a respiratory rate of 660 breaths/min. 240 and 420 (????) breaths/min and changes to the oxygenation index at the rate of 420 breaths/minute (Figure??). Subsequent post-hoc analysis by the Wilcoxon test (Table 27) with the adjusted degree of relevance then confirmed statistically significant changes between groups without the orifice plate (none) and with the orifice plate (Sens) for PaCO\({}_{2}\) and pH parameters at rates of 240 and 420 breaths/min (??? ?, Table??). The difference in PaCO\({}_{2}\) at a respiratory rate of 240 breaths/min (Figure??) between groups with an orifice (Sens) and with orifice plate and compensation (SensComp) was also statistically significant. These results point to the significant impact on PaCO\({}_{2}\) and pH parameters when inserting the orifice plate into the ventilation circuit. After increasing PIP pressure amplitude (compensating pressure loss--SensComp situation), a statistically significant change was observed only in the PaCO\({}_{2}\) parameter at a respiratory rate of 240 breaths/min. Statistically significant changes to the oxygenation index were observed at 420 breaths/min between the groups none and SensComp (Figure??). This situation was not expected in advance.