## 2 Results and Discussion
#### 2.1.1 Characteristics of Selected _Brassicaceae RSH_ Genes  
In silico studies are often used as a preliminary means of analysis of plant gene families that enable the capturing of the phylogenetic relationships within a family of genes in one species, as well as between species [36; 37; 38; 39; 40]. A total of 45 _RSH_ genes that were identified were selected for this study of _Brassicaceae_ (_B. napus_, _B. oleracea_, _B. rapa_, _Camelia sativa_, and _Raphanus sativus_) plants are shown in Table 1. _B. napus_ is an allotetraploid species and thus, as expected, has more _RSH_ orthologous genes (14 in total, including 2 pseudogenes) than _A. thaliana_, where only 4 _RSH_ genes have been described [35; 41]. Four _RSH_ genes are present also in the _B. rapa_ genome, whereas in the genome of _B. oleracea_ 6 _RSH_ genes occur, and in the genome of _R. sativus_ 8 _RSH_ genes are present, though all these plants are diploids. In the allohexploid genome of _C. sativa_ 12 _RSH_ genes are present, however, 3 of them are pseudogenes. In _O. sativa_, one gene in each of the _RSH1_, _RSH2_, and _RSH3_ subgroups, and three _CRSH_ genes were identified [42]. In _I. nil_, five _RSH_ genes were identified, i.e., 1 _RSH1_, 2 _RSH2_, 1 _RSH3_, and 1 _CRSH_[25]. Genes encoding _RSH_ were described also in _Capsicum annu_[43], _Nicotiana tabacum_[44], and _Suada japonica_[45]. \begin{table} \begin{tabular}{c c c c c c c c c c c c c c} \multicolumn{11}{c}{Table 1. Cont.} & & & & & & & & & & & & \\ \hline
**Spodies** & **Cenes** & **Gene ID** & **Transcript ID** & **CDS** & **Chromosome** & **Protein ID** & **AA** & **pI** & **Mw** & **tOD** & **Intross** & **Exons** & **Predicted Transfer Peptide** \\  & & & & & & & & & & & & & & & **(Probability)** \\ \hline  & RSHI\_a & 100474094 & XM,0100468670.2 & 2664 & 2 & \(\chi\)/D10468670.1 & 887 & 6.66 & 98.83 & 24 & 25 & & & & & & & \\ \hline  & RSHI\_b & 100473558 & XM,010485775.2 & 2653 & 13 & \(\chi\)/D10468670.1 & 884 & 6.56 & 98.56 & 25 & 26 & & & & & \\ \hline  & RSHI\_b & 100473057 & - & - & 8 & - & - & - & - & - & - & - & - & - \\  & -pseudo & & & & & & & & & & & & & & & \\  & RSHI\_a & 100477842 & XM,01003407.2 & 2154 & 1 & \(\chi\)/D10501569.1 & 717 & 6.57 & 79.77 & 6 & 7 & & & & & & \\ \hline  & RSHI\_b & 10047886263 & XM,010513997.2 & 630 & 5 & \(\chi\)/D10512299.1 & 209 & 7.22 & 23.53 & 3 & 4 & & & & & & \\ \hline  & RSHI\_c & 1004745674 & XM,010468670.2 & 2148 & 15 & \(\chi\)/D10468670.1 & 715 & 6.42 & 79.56 & 6 & 7 & & & & & & \\ \hline  & RSHI\_a & 100478355 & XM,010502782.2 & 2151 & 3 & \(\chi\)/D10501084.1 & 716 & 6.19 & 80.09 & 5 & 6 & & & & & & \\ \hline  & RSHI\_b & 1004788764 & XM,010487702.2 & 2151 & 17 & \(\chi\)/D104880004.1 & 716 & 6.77 & 79.75 & 5 & 6 & & & & & & \\ \hline  & RSHI\_c & 100472935 & - & - & 14 & - & - & - & - & - & - & - & - \\ \hline  & RSHI\_c & 1004761544 & - & - & 18 & - & - & - & - & - & - & - & - \\ \hline  & RSHI\_a & 1004782095 & XM,010506922.2 & 1764 & 1 & \(\chi\)/D10505224.1 & 587 & 6.20 & 66.97 & 3 & 4 & & & & & \\ \hline  & RSHI\_b & 1004765992 & XM,01048935.2 & 1758 & 19 & \(\chi\)/D1048670.1 & 585 & 6.07 & 66.89 & 3 & 4 & & & & & \\ \end{tabular}   
_B. napus RSH_ genes are distributed in 9 out of 19 chromosomes (Figure 1), but one of the _BnRSH1_ genes has not yet been assigned to any chromosome. In _B. oleracea_, _RSH_ genes are located on 5 out of 9 chromosomes, and in _B. rapa_ the _RSH_ genes are located on 4 out of 10 chromosomes. There are no differences between the number and the localization of _RSH_ genes on chromosomes in _B. oleracea_ and on C-genome chromosomes in _B. napus_. In the case of A-genome chromosomes, there are additional _RSH1_ genes on chromosome A5 and A9 in comparison with the genome of _B. rapa_. Moreover, the _CRSH_ gene located on chromosome A3 is a pseudogene in _B. napus_. The presence of an _RSH3_ pseudogene located on chromosome A6 could be caused by genome assembly errors since both genes lies in proximity and are separated by an unknown sequence.