## 3 Results and Discussion
The results of the Reactome pathway analysis revealed that _FCERIG_ was enriched in platelet adhesion for collagen exposure and lectin-2 family and neutrophil degranulation, while BP showed enrichment in neutrophil degranulation, neutrophil activation and myeloid cell activation when involved in immune response (Tables S12 and S13). Fc Gamma Receptor IIIa (_FCER3A_) encodes a receptor for the Fc portion of immunoglobulin G and participates in the removal of antigen-antibody complexes from the circulation, antibody-dependent cellular mediated cytotoxicity, and the antibody-dependent enhancement of virus infections. _FCER3A_ was recently detected as a significant contributor to peripheral blood nucleated cells derived from AD patients in an integrated pipeline based on single-cell analysis [41]. In BP, it was found to be significantly enriched in peptide cross-linking and the regulation of inflammatory responses. _PELI1_ (Pellino E3 Ubiquitin Protein Ligase 1) is involved in the negative regulation of the necrotic process as well as in protein polyubiquitin and promotes responses to lipopolysaccharide. Its expression can be detected in murine brain neural cells, with the highest expression level being in the microglia [42]. It is also involved in microglial A\(\beta\) phagocytosis by inhibiting the expression of scavenger receptor CD36, which has been demonstrated as a potent therapeutic target against neurodegenerative diseases. [43]. According to GO analysis, BP _PELI1_ enrichment is depicted in the Toll signaling pathway, the regulation of the necrotic process and protein K63- and K48-linked ubiquitination (Table S14). The important role of E3 ubiquitin ligases and deubiquitining enzymes in the pathogenesis mechanisms of neurodegenerative diseases have been extensively analyzed, such as their association with a-synuclein in [PERSON]'s disease, as well as their deficiency in mitophagy and mitochondrial dynamics in relation to [PERSON]'s response [44]. _ZEB2,_ which encodes the Zinc Finger E-Box Binding Homeobox 2 protein, significantly enriched the regulation of transforming growth factor beta receptor signaling pathway and regulation of organelle organization (Table S15). Upregulated levels of this transcription factor have been detected in the prospective neural plate and induced the expression of _Sox2_ and _Ncam_ neural-specific genes while participating in the differentiation of granule neurons in the cerebellum and together with _miR-200c_ as maintain the balance between midbrain dopaminergic progenitor proliferation and neurogenesis [45]. _NR4A2_ (nuclear receptor subfamily 4 group A member 2) gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily, which acts as a nuclear receptor and a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including [PERSON]'s disease, AD and multiple sclerosis. It has been linked with neuroinflammation, neuronal cell death as well as neuroprotection, and its expression has been reduced in PD post-mortem brains in PD patients. It regulates dopaminergic neuronal differentiation and also localizes in microglia and astrocytes [46]. _NR4a2_ is induced by neuronal activity and regulates hippocampal synaptic plasticity and hippocampal-dependent memory [47]. The results of GO analysis indicated that _NR4A2_ in BP was enriched in midbrain dopaminergic neuron differentiation, dopaminergic neuron differentiation, central nervous system neuron differentiation, regulation of apoptotic signaling pathway and neuron migration (Table S16). The functional role of the integrin alpha4 subunit (_ITGA4_) in neural crest cell migration has been detected [48] as well as an anti-integrin therapeutic strategy against multiple sclerosis has been described [49] and was found significantly enriched in integrin cell surface interactions in immunoregulatory interactions between a lymphoid and a non-lymphoid cell and extracellular matrix organization pathways (Table S17).