## 3 Results
### Extraction of Volatiles and Analysis by GC-MS and GC-FID/GC-MS
#### 3.1.2 GC-MS and GC-FID/GC-MS Analysis of DHS Collection
\begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline \multicolumn{2}{c}{**Compound**} & \multicolumn{5}{c}{**Area \%**} \\ \hline \multirow{2}{*}{**RRI**} & \multirow{2}{*}{**Name**} & \multirow{2}{*}{**Hydrodistillate**} & \multicolumn{5}{c}{**Headspace**} & \multirow{2}{*}{**Identification**} & \multirow{2}{*}{**References**} \\ \cline{3-4} \cline{6-7}  & & & & **HS-SPME** & & \\ \cline{3-4} \cline{6-7}  & & & **PDMS/DVB** & **CAR/PDMS** & **PDMS** & \\ \hline
1087 & 2-hexanone \({}^{\text{b}}\) & 7.9 & & & & MS & [32] \\ \hline
1093 & hexanal & & 9.0 & 16.9 & RRI, MS & [37] \\ \hline
1155 & 1-butanol & & trace & 4.8 & RRI, MS & [11] \\ \hline
1194 & heptanal & & 13.2 & 13.9 & RRI, MS & [8,37] \\ \hline
1202 & 3-hexanol \({}^{\text{b}}\) & 2.7 & & & MS & [37] \\ \hline
1212 & isoamyl alcohol & & 5.2 & 12.1 & MS & - \\ (=3-methyl-1-butanol) \({}^{\text{b}}\) & & & & MS & [36] \\ \hline
1222 & 2-hexanol \({}^{\text{b}}\) & 4.6 & & & MS & [32] \\ \hline
1260 & 1-pentanol & & 3.0 & 6.4 & RRI, MS & [32] \\ \hline
1290 & 2-octanone \({}^{\text{a,b}}\) & & 1.6 & 1.0 & MS & \\ \hline
1296 & octanal \({}^{\text{a}}\) & & 5.0 & 3.2 & RRI, MS & [8,37] \\ \hline
1300 & tridecane \({}^{\text{a}}\) & & 10.3 & & & RRI, MS & [30] \\ \hline
1360 & 1-hexanol & & 6.9 & 9.6 & RRI, MS & [32] \\ \hline
1400 & tetradecane \({}^{\text{a}}\) & 3.1 & & & & RRI, MS & [36] \\ \hline
1400 & nonanal \({}^{\text{a}}\) & & 10.6 & 8.0 & 1.9 & 6.6 & RRI, MS & [8,37] \\ \hline
1412 & (\(E\))-2-hexenol \({}^{\text{b}}\) & & & 1.3 & 1.0 & MS & - \\ \hline
1463 & 1-heptanol & & 2.4 & 3.3 & 3.4 & RRI, MS & [35] \\ \hline
1496 & 2-ethyl hexanol \({}^{\text{a,b}}\) & & 21.0 & 3.9 & 3.2 & MS & [10] \\ \hline
1496 & 2-decanone \({}^{\text{b}}\) & 0.5 & & & & MS & [32] \\ \hline
1500 & pentadecane \({}^{\text{a}}\) & 0.5 & 5.4 & & & RRI, MS & [30,36] \\ \hline
1541 & benzaldehyde & & & 1.0 & 0.5 & RRI, MS & [11] \\ \hline
1562 & 1-octanol \({}^{\text{a}}\) & 0.4 & 5.7 & 2.2 & 0.9 & RRI, MS & [36] \\ \hline
1583 & longifolene (=junipene) \({}^{\text{a,b}}\) & & 6.9 & & & MS & [34] \\ \hline
1600 & hexadecane & 0.3 & 3.0 & & & RRI, MS & [30,36] \\ \hline
1604 & 2-undecanone \({}^{\text{b}}\) & 0.2 & & & & MS & [32] \\ \hline
1612 & \(\beta\)-caryophyllene \({}^{\text{a}}\) & & 6.2 & & & RRI, MS & [35] \\ \hline
1614 & 3,5-dimethyl-1,2,\(A\)-trithiolane & & & & & MS & [30,31] \\ \cline{3-4} \cline{6-7}  & (one of the isomers) \({}^{\text{a,b}}\) & & & & & MS & [30,31] \\ \cline{3-4} \cline{6-7}
1631 & \(\gamma\)-pentalactone \({}^{\text{(}}\)=\(\gamma\)-valerolactone) \({}^{\text{b}}\) & & & 1.1 & 0.7 & - & MS & [39] \\ \hline
1634 & 3,5-dimethyl-1,2,\(A\)-trithiolane & & & & & MS & [30,31] \\ \cline{3-4} \cline{6-7}  & (one of the isomers) \({}^{\text{a,b}}\) & 0.6 & & & & MS & [30,31] \\ \hline
1664 & 1-nonanol & 0.9 & 1.3 & 0.5 & - & - & RRI, MS & [12] \\ \hline
1674 & 2-methylbutanoic acid \({}^{\text{b}}\) & & & 2.1 & 3.1 & 3.5 & MS & [37] \\ \hline
1700 & heptadecane & & 1.1 & & & & RRI, MS & [30,36] \\ \hline
1706 & \(\alpha\)-terpineol & & & 0.6 & - & - & RRI, MS & - \\ \hline \multirow{4}{*}{1762} & 5,6-dihydro-2,\(A\)-6-trimethyl- & & & & & RRI, MS & [30,33] \\  & 4H-1,3,5-dithiazine (thialdine, base peak 163) \({}^{\text{a}}\) & & & & & RRI, MS & [30,33] \\ \hline
1800 & octadecane & & 2.4 & & & RRI, MS & [30,36] \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Chemical composition of sheep wool hydrodistillate, DHS and HS-SPME volatile collections analyzed by gas chromatography–flame ionization detector (FID) combined with GC–MS. octen-3-ol (0.75 \(\pm\) 0.02 at 15 min; 0.75 \(\pm\) 0.02 at 30 min; 0.81 \(\pm\) 0.05 at 60 min). Similarly, co-application with 10 mL of CO\({}_{2}\) showed little attractancy at any time point to _Ae. aegypti_ mosquitoes when tested at 0.1 and 0.01 \(\upmu\)g/cm\({}^{2}\) of sheep wool hydrodistillate. Thus, according to the assay results, the hydrodistillate and thialidine did not show any significant attractancy against female adult _Ae. aegypti_.