## 3 Results  
SMF Regulates the Ability of HD Water Pre-Exposed to a Magnetic Field to Enhance ROS Production by Neutrophils  
Weak and ultra-weak magnetic fields have pronounced biophysical effects, associated with the formation of free radicals and respiratory burst [12; 13; 14; 15]. Earlier, we showed that the treatment of water with a CMF (SMF of 60 uT, AMF of 150 nT, and the variable component frequency of 12.6 or 48.5 Hz) affects the ability of these samples to regulate the activity of neutrophils to produce ROS in different ways. This ability persists even if this water is diluted to the C50 level, but disappears if dilutions are prepared in a near-zero magnetic field or without vibration between dilution steps [11]. However, it has remained unexplored how the ability of water to activate neutrophils after exposure to a magnetic field depends on the level of dilution of water and, most importantly, on the parameters of the static component of the magnetic field, at which a series of dilutions is prepared. This dependence was investigated in the current study using luminol-enhanced cellular chemiluminescence. First, it was necessary to show the stability of the stimulating effect of water treated with a CMF (SMF of 60 uT, AMF of 50 nT, and a frequency of a variable component of 12.6 Hz) in terms of its ability to activate the production of ROS by neutrophils. Table 1 shows data from four independent series of experiments on the reproducibility of this effect; the experiments were conducted on different days. Pre-treatment of water with the CMF for 90 min followed by its addition to concentrated Hanks solution and further to the neutrophil suspension resulted in the stimulation of the response of these cells to the respiratory burst activator fMLF by approximately 40-50%, compared to the addition of water kept under ambient conditions (at the GMF). The reproducibility of the results in general indicates the stability of the stimulating effect of water pre-treated with the CMF on ROS production by neutrophils. It is important to note that the testing of water samples in these series of experiments began no later than 30 min after the end of their exposure to the CMF. An additional control was tested to confirm the effect of the CMF on the ability of water to enhance ROS production by neutrophils. When water was incubated under GMF conditions (i.e., instead of the sample pre-exposed to the CMF, as shown above), it did not \begin{table}
\begin{tabular}{c c c c} \hline \hline \multirow{2}{*}{**Experiment No.**} & \multicolumn{3}{c}{**Chemiluminescence Intensity, V**} \\ \cline{2-4}  & **Control** & **Test** & _p_**-Value** \\ \hline I & 5.17 \(\pm\) 0.37 (100 \(\pm\) 7.2\%) & 7.16 \(\pm\) 0.84 (138.5 \(\pm\) 16.2\%) & 0.00034 \\ \hline II & 5.34 \(\pm\) 0.75 (100 \(\pm\) 14.0\%) & 7.51 \(\pm\) 0.86 (140.6 \(\pm\) 16.1\%) & 0.00216 \\ \hline III & 4.26 \(\pm\) 0.85 (100 \(\pm\) 19.9\%) & 5.99 \(\pm\) 0.79 (140.6 \(\pm\) 18.5\%) & 0.00420 \\ \hline IV & 3.30 \(\pm\) 0.25 (100 \(\pm\) 7.6\%) & 4.96 \(\pm\) 0.81 (150.3 \(\pm\) 24.5\%) & 0.00320 \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Chemiluminescence intensity of neutrophil suspension after addition of water pre-treated with the CMF (SMF of 60 uT; AMF of 50 nT, 12.6 Hz; exposure time of 1.5 h) to the culture medium. n = 6 for each experiment. The measurements of the maximum of chemiluminescence intensity are presented as Mean \(\pm\) Standard deviation, and additionally, in the brackets results of normalized chemiluminescence are presented. Normalized chemiluminescence was calculated by dividing the maximum chemiluminescence value measured for a particular sample by the maximum value obtained for water. The value of chemiluminescence for the “Water” sample (Control) was taken as 100%. exert a stimulating effect (only 102.2\(\pm\) 7.4%) and did not differ (\(p\) = 0.5) in this parameter from the intact (and control--100 \(\pm\)16.4%) water. If the effect on aqueous solutions is more of a physical nature, it should depend on time.