## 1 Introduction  
Bone graft materials are essential for treatment in oral and maxillofacial surgery because of the implant unavailability for bone replacement [1]. The development of bone graft materials has increased the available bone regeneration technologies in the dental field. The use of bone grafts during immediate post-extractive implant placement has shown better aesthetic results [2]. Autogenous bone graft is regarded as standard for bone regeneration in considerable peri-implant bone defects [3]. However, this approach is limited when the volume of the defect exceeds the amount of attainable graft and donor site morbidity [4]. Therefore, alternatives to autogenous bone grafts are needed [5]. Various bone graft materials that promote adequate bone healing have been reported in the field of oral and maxillofacial surgery [6]. Allogeneic, xenogeneic, and synthetic bone have been developed and used as bone substitutes in oral and maxillofacial surgery [7, 8]. Experiments to identify the best bone graft material are underway. Furthermore, a bone graft material derived from autogenous tooth has been developed [8]. A tooth-derived bone graft or tooth biomaterial is a type of autogenous bone graft fabricated from an extracted tooth with a demineralization process. Tooth biomaterial may be useful as a graft material for bone regeneration because of its osteeconductive properties [9]. a previous study showed that the primary tooth pattern is formed by hydroxyapatite (HA) as well as small amounts of \(\beta\)-tricalcium phosphate, octacalcium phosphate, and amorphous calcium phosphate; although the level of HA crystallization varies according to the area of the tooth [9]. HA can be degraded and absorbed in vivo. Bone and teeth have a very similar chemical composition. The inorganic and organic content of dentin and alveolar bone are alike so it can easily replace autogenous bone grafts. The only difference is the method of harvesting the graft. While harvesting a bone graft from other sites can cause recipient site morbidity, tooth bone graft can be easily prepared from a non-restorable or third molar extracted tooth. Tooth biomaterial is biocompatible and has been used as a bone grafting material to regenerate bony defects [10]. Autogenous bone grafts have been used in combination with bioactive glass [11] and bovine bone grafts [12] to enhance the bone volume fraction. When autogenous bone graft is used in combination it offers certain advantages like increased volume of the graft and obviates the need for large amounts of autogenous bone [13]. Bone grafting techniques using platelet-rich plasma (PRP) have been shown to improve bone healing in maxillofacial surgery [14; 15]. Some studies successfully applied PRP in bone grafting surgery [16; 17]. Various growth factors released from PRP, like platelet-derived growth factor, insulin-like growth factor, and epidermal growth factor have bone regeneration effects on peri-implant bone grafts [18; 19]. However, some studies showed that PRP did not have bone regeneration effects on peri-implant bony defects [20]. Platelet-rich fibrin (PRF) is a second-generation platelet concentrate that can be isolated by centrifugation from autogenous blood. PRF is an autogenous fibrin clot rich in platelets and does not require factors such as bovine thrombin [21]. The use of PRP gels (fibrin) in bone defects does not enhance healing of the bone defect compared to non-treated defects [22]. However, the application of additional fibrin with biomaterial can increase bone regeneration compared to biomaterial alone. Significant fibrin sealant-mediated enhancement of bone repair was observed in a previous study [23]. Although platelet cytokines play an important role in enhancing bone regeneration, the fibrin matrix that supports these molecules determines the element responsible for the therapeutic potential of PRF [24; 25]. Tooth biomaterial is a useful source of bone graft material for increasing bone regeneration effects.