## 4 Discussion  
Currently, more and more new techniques have been developed for bacterial identification. Although MALDI-TOF MS has become a widely used technique for the rapid, accurate, durable, economical, and trustworthy method of bacteria identification [28], 165 rRNA gene sequencing, as an amplicon-based method that can identify and classify bacteria, has been used simultaneously with the next-generation sequencing technique (NGS). BLAST (basic local alignment search tool) finds regions of local similarity between sequences. The program compares nucleotide or protein-sequences databases and calculates the statistical significance of matches. In our case, sequencing led to the taxonomic allotment of the raw goat milk-derived strain MK1/3 as _Lactococcus lactis_. The species _Lactococcus lactis_ is characterized as not exhibiting \(\beta\) and/or \(\alpha\)--hemolysis [29], which was also shown in the MK1/3 strain. Regarding the enzyme production, \(\beta\)-galactosidase is important for an organism, as a key provider in the production of energy and as a source of carbons through the breakdown of lactose to galactose and glucose. It is important for lactose-intolerant people, as it is responsible for making lactose-free milk and other dairy products. In the case of lactase lack (which has the same function as \(\beta\)-galactosidase), a human is not able to properly digest dairy products. Recently, \(\beta\)-galactosidase has been researched as a potential treatment for lactose-intolerance through gene replacement therapy; genes can be placed into human DNA and individuals can break down lactose on their own [30]. The damaging enzyme \(\alpha\)-chymotrypsin serves as a disease marker and \(\beta\)-glucuronidase can even be detected in the case of colon cancer. If the selected strain is to be used as beneficial additive, it has to fulfill some safety criteria. Among them is included the absence of antibiotic-resistance genes. In our study, an antibiotic phenotype was evaluated (not genes), but the strain MK1/3 showed antibiotic susceptibility, when testing it by two approaches. In the other study, [PERSON] et al. [31] described MIC for _L. lactis_ strains isolated from dairy and animal sources using the E-test. They found those strains susceptible to vancomycin, erythromycinycin, streptomycin, chloramphenicol, tetracycline, and clindamycin. The most important point for a further functional strain is its in vivo safety. In this study, in vivo safety of the strain MK1/3 was tested using the Balb/c mice model. No mortality was noted. The strain MK1/3 was sufficiently established in the mice GIT, with the highest value on day 90 (4.38 \(\pm\) 1.24 CFU/g) and with significant increases during whole testing. A significant increase in _Enterococci_ was also found (in E90 compared with \begin{table}
\begin{tabular}{c c c c c} \hline  & **pH/Ewe** & **pH/Combinative** & \
\begin{tabular}{c} **MK1/3** \\ **\#Ewe + Goat** \\ \end{tabular} &
\begin{tabular}{c} **MK1/3** \\ **\#Ewe** \\ \end{tabular} \\ \hline E/24 & 3.28 \(\pm\) 0.20 & 3.91 \(\pm\) 0.10 & 4.61 \(\pm\) 0.20 & 1.95 \(\pm\) 0.25  \\ \hline C/24 & 3.38 \(\pm\) 0.83 & 3.93 \(\pm\) 0.10 & nt & nt \\ \hline E/7 & 3.30 \(\pm\) 0.20 & 4.10 \(\pm\) 0.50 & 4.43 \(\pm\) 0.30 & 1.95 \(\pm\) 0.25  \\ \hline C/7 & 3.35 \(\pm\) 0.54 & 4.09 \(\pm\) 0.20 & nt & nt \\ \hline E/10 & 3.31\(\pm\) 0.10 & 3.90 \(\pm\) 0.10 & 4.66 \(\pm\) 0.30 & 4.10 \(\pm\) 0.10  \\ \hline C/10 & 3.40 \(\pm\) 0.60 & 3.85 \(\pm\) 0.10 & nt & nt \\ \hline \end{tabular}
\end{table} 
Table 5: The counts of encapsulated MK1/3 strain in pygurts from ewe-goat milk and ewe milk (CFU/g, log 10 \(\pm\) SD) and pH values at different days of storage. E30, and 120). Moreover, LAB counts and amyloidtic cocci, as well, were well-balanced. Coliforms were not influenced, and reduction in _Staphylococci_ (mathematical difference up to significant difference) was noted.