## 3 Results
### Influence of the Relative Substrate Concentrations on Oxidant Levels and Antifungal Activity  
#### 3.4.1 Influence of the Relative Substrate Concentrations on Oxidant Levels  
We classified the relative iodide/thiocyanate concentrations in three categories: \(<\)1 (Figure 4), \(=\)1 (Figure 5) and \(>\)1 (Figure 6), and measured the influence of each ratio through the level of produced oxidant. When thiocyanate is the major substrate present in the media (KI/KSCN ratio \(<\) 1; Figure 4), oxidant production stayed low unless thiocyanate was added at a high concentration (10 mM KSCN). The capacity to oxidize the \(-\)NH\({}_{2}\) group remained low, with a maximum concentration at 25 uM, even with a high iodide concentration. As the NH\({}_{2}\)-dosage was very low and clearly different from SH-dosage, it can be hypothesized that SH-dosage is predominantly due to oxidized thiocyanate. However, even with 10 mM KSCN, the SH-dosage reached only 250 uM, which is low when compared with 350 uM OSCN\({}^{-}\) produced with 1.2 mM KSCN as the sole substrate (Table 2). The addition of iodide act like an inhibitor of OSCN\({}^{-}\) production. Three ratios showed this clearly: (i) 1.2 mM KI + 5.4 mM KSCN, (ii) 1.2 mM KI + 4.05 mM KSCN, and (iii) 1.2 mM KI + 1.35 mM KSCN, which represent 4.5, 3.37, and 1.12, respectively, more thiocyanate than iodide, as well as where OSCN\({}^{-}\) production increased with thiocyanate quantity and NH\({}_{2}\)-dosage increased with iodide concentration relatively to thiocyanate. The lactoperoxidase system that used a mixture of iodide and thiocyanate in \begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline  & & \multicolumn{6}{c}{**Storage Duration (Days)**} \\ \cline{3-8}  & & **0** & **30** & **90** & **120** & **180** & **240** & **300** & **360** \\ \hline
**Temp.** & **Water Mineral.** & \multicolumn{6}{c}{_P. expansum_ **Inhibition (\%)**} \\ \cline{3-8}  & **Low** & \(94\pm 3\) & \(14\pm 10\) & \(0\pm 0\) & \(0\pm 0\) & \(10\pm 4\) & \(0\pm 1\) & \(0\pm 0\) & \(2\pm 1\) & \(0\pm 0\) \\
**25 \({}^{\circ}\)C** & **M. Low** & \(93\pm 4\) & \(0\pm 0\) & \(0\pm 0\) & \(8\pm 2\) & \(8\pm 2\) & \(6\pm 2\) & \(1\) & \(8\pm 3\) & \(3\pm 2\) \\
**25 \({}^{\circ}\)C** & **M. High** & \(91\pm 2\) & \(88\pm 8\) & \(0\pm 0\) & \(0\pm 0\) & \(4\pm 4\) & \(5\pm 3\) & \(3\pm 3\) & \(9\pm 4\) & \(4\pm 2\) & \(2\) & \(1\) \\  & **High** & \(91\pm 4\) & \(87\pm 5\) & \(88\pm 1\) & \(0\pm 0\) & \(0\pm 0\) & \(0\pm 0\) & \(3\pm 3\) & \(3\pm 3\) & \(0\pm 1\) & \(0\pm 0\) \\ \hline
**4 \({}^{\circ}\)C** & **Low** & \(99\pm 1\) & \(88\pm 4\) & \(90\pm 0\) & \(91\pm 1\) & \(88\pm 3\) & \(94\pm 1\) & \(31\pm 10\) & \(59\pm 5\) & \(a\) \\
**4 \({}^{\circ}\)C** & **M. Low** & \(98\pm 2\) & \(89\pm 4\) & \(89\pm 3\) & \(91\pm 2\) & \(87\pm 2\) & \(6\pm 3\) & \(78\pm 2\) & \(6\pm 3\) & \(1\pm 1\) & \(10\pm 3\) \\
**M. High** & \(99\pm 0\) & \(86\pm 3\) & \(88\pm 3\) & \(92\pm 2\) & \(85\pm 4\) & \(92\pm 2\) & \(90\pm 5\) & \(89\pm 4\) & \(b\) \\
**High** & \(98\pm 2\) & \(90\pm 6\) & \(88\pm 4\) & \(90\pm 3\) & \(84\pm 3\) & \(91\pm 0\) & \(97\pm 4\) & \(b\) & \(92\pm 5\) & \(b\) \\ \hline \hline \end{tabular}
\end{table} 
Table 6: Percentage of inhibition of _P. expansum_ growth by double-dose solutions (10.8 mM KI + 2.4 mM KSCN + 13.2 mM H\({}_{2}\)O\({}_{2}\)) after lactoperoxidase oxidoreductase induction in low- to high-mineralized waters and storage at 25 \({}^{\circ}\)C or 4 \({}^{\circ}\)C for 360 days. [PERSON]’s comparison of means was applied with 95% confidence; means that do not share a letter are significantly different; ns, not significantly different from the control. Temp. : storage temperature; Water mineral. : water mineralization; M. Low: medium low; M. High: medium high. Means and s.d. of two independent experiments are given. \begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline  & & \multicolumn{6}{c}{**Storage Duration (Days)**} \\ \cline{3-8}  & & **0** & **30** & **90** & **150** & **240** & **300** & **360** \\ \hline
**Temp. ** & **Water Mineral.