## 4 Discussion  
Lettuce is one of the most popular vegetable plants used in the human diet. Leaves contain polyphenolic compounds, vitamins and calcium and iron. When eaten raw, lettuce can be a source of antioxidants in the human diet, preventing chronic diseases. It can be grown both in the field and in greenhouses. It generally belongs to a temperate, cool climate and is a good vegetable in such conditions [58]. It is expected that the production \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Water Regime** & **Fertilizer** & **Dose** & **Riboflavin** & **\(p\)-HyDroxybenzoic** & **Chlorogenic** & **Ferulic Acid** &
\begin{tabular}{c} **\(o\)-Cumaric Acid** \\ **Acid** \\ **(ug/g dm)** \\ \end{tabular} \\ \hline \multirow{4}{*}{Watered} & Control & - & 0.006 c,A & 0.472 c,A & 2303 a,A & 4548 a,A & 1.011 b,B \\  & Cattle & 10 & 0.006 c,A & 0.704 a,A & 1401 b,A & 2825 b,A & 1.136 a,B \\  & manure & HI frass & 10 & 0.009 b,A & 0.733 a,A & 1190 c,A & 2545 b,A & 1.135 a,B \\  & HI frass & 12.5 & 0.013 a,A & 0.576 b,A & 26.3 d,B & 692 c,B & 1.079 b,B \\ \hline \multirow{4}{*}{Drought} & Control & - & 0.004 a,A & 0.460 a,A & 531 b,B & 1667 a,B & 1.650 a,A \\  & Cattle & 10 & 0.004 a,B & 0.386 c,B & 568 a,B & 1318 c,B & 1.463 b,A \\ \cline{1-1}  & H frass & 10 & 0.004 a,B & 0.412 b,B & 426 c,B & 1292 c,B & 1.639 a,A \\ \cline{1-1}  & HI frass & 12.5 & 0.005 a,B & 0.463 a,B & 570 a,A & 1506 b,A & 1.514 ab,A \\ \hline \hline \end{tabular}  
* The results noted with different lowercase letters differ statistically at the level of \(\alpha=0.05\) between the fertilizers used. The results noted with different uppercase letters differ statistically at the level of \(\alpha=0.05\) between watering methods and appropriate fertilizers. Statistical difference for each parameter and water condition calculated separately.  
\end{table} 
Table 6: Lettuce polyphenolic compounds. \begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Water Regime** & **Fertilizer** & **Dose** & **Ce** & **NCER** \\  & **(g/L)** & **(vpm)** & **(umol m\({}^{-2}\) s\({}^{-1}\))** \\ \hline \multirow{4}{*}{Watered} & Control & - & 11.9 c & 1.2 c \\  & Cattle manure & 10 & 82.9 ab & 8.7 ab \\  & HI frass & 10 & 130.1 a & 13.6 a \\  & HI frass & 12.5 & 127.5 a & 13.4 a \\ \hline \multirow{4}{*}{Drought} & Control & - & 1.7 c & 0.18 c \\  & Cattle manure & 10 & 20.2 c & 2.1 c \\  & HI frass & 10 & 29.0 c & 3.1 c \\ \cline{1-1}  & HI frass & 12.5 & 31.9 bc & 3.4 bc \\ \hline \hline \end{tabular}  
* Letters a-c indicate statistically different mean values (\(\alpha=0.05\)). Ce: LSD Fertilizer: 5.422; LSD Water regime: 30.249; LSD Fertilizer \(\times\) Water regime: NS, NCER: LSD Fertilizer: 1.443; LSD Water regime: 31.597; LSD Fertilizer \(\times\) Water regime: NS.  
\end{table} 
Table 7: Soil respiration under different fertilizer and water conditions. of insects for protein purposes will likely increase significantly in the coming years. Along with this, the production of frass excrement from this production, which will be used for agriculture, will also increase. The use of frass in lettuce fertilization is one of its possible uses [59]. It has also been used as an addition to soilless substrate in the production of tomatoes, lettuce and basil [60]. In the conducted experiment, frass application did not influence the fresh mass of lettuce. There could be many reasons for this--the short vegetation period of the plant, which did not allow for the observation of the fertilization effect when too small or too large doses were used. We also find similar results from other authors. High doses of frass fertilization may inhibit plant growth and reduce plant yields. This is due to the toxicity of NH\({}_{4}\). This effect may also be allelopathic [61]. The research showed that frass had various effects on the yield and mineral composition of lettuce leaves. Also, no increase in the yield was observed even at higher doses [62].