# Effect of Montmorillonite on 4-Nonylphenol Enrichment in Zebrafish
## 3 Results
### The Determination Results and Data Analysis of 4-NP in Tissues
#### 3.4.1 Variance Analysis of 4-NP Contents in Liver  
Tables 3-5 represent the significance analysis of the mean difference of the 4-NP contents in liver at Day 7, Day 15 and Day 30 between the groups (N1, N2 and N3 respectively represent low, medium and high contents of 4-NP, and M1, M2 and M3 respectively represent low, medium and high concentrations of MMT. In addition to the row of 4-NP content, other columns with the asterisk (*) represents the \(p\)-value, where \"/\" represents \(p\geq 0.05\), * represents \(p<0.05\), ** represents \(p<0.01\) and blank represents no comparison.). The changes of 4-NP contents of each experimental group at Day 7, Day 15 and Day 30 are shown in Figure 1 (* and \"/\" at the top of the figure: the first row represents the analysis of the difference of significance between 4-NP contents at Day 7 and Day 15, the second row represents analysis at 7 d and 30 d, while the third row represents analysis at Day 15 and Day 30). The data analysis showed that whether with or without MMT, the concentration of 4-NP in liver has been on the decline over time. On the seventh day, when MMT was non-existent, the concentration of 4-NP in liver was highly enriched with a low dosage, but there was no such effect at Day 15 and Day 30. By contrast, 4-NP cannot be enriched in liver at any time while MMT exists.  
\begin{table}
\begin{tabular}{c c c} \hline \hline
**Samples** & **Equations** & **Correlation Coefficient (R2)** \\ \hline Water & y = 370.65x + 431,635 & 0.9965 \\ Liver & y = 362.77x + 356,899 & 0.9931 \\ Muscle & y = 366.87x + 400,058 & 0.9922 \\ Gill & y = 361.34x + 451,265 & 0.9909 \\ \hline \hline \end{tabular}
\end{table}
Table 2: Regression equations and correlation coefficients of the standard curves.  
\begin{table}
\begin{tabular}{c c c c c c c c c c c c} \hline \hline  & **4-NP Contents (ug/g)** & **N2** & **N3** & **NiM1** & **NiM2** & **NiM3** & **N2M1** & **N2M2** & **N2M3** & **N3M1** & **N3M2** & **N3M3** \\ \hline N1 & 0.0347 \(\pm\) 0.0039 & ** ** ** & ** & ** & / & / & ** & ** & / & & \\ N2 & 0.0422 \(\pm\) 0.002 & ** ** & ** & & & & & ** & ** & / & \\ N3 & 0.0517 \(\pm\) 0.0022 & & & & & & & ** & / & ** & \\ N1M1 & 0.0231 \(\pm\) 0.0068 & & ** & ** & ** & ** & & & * & & \\ N1M2 & 0.0358 \(\pm\) 0.0029 & & & & & / & & / & & / & \\ N1M3 & 0.0401 \(\pm\) 0.0021 & & & & & & / & & * & \\ N2M1 & 0.0253 \(\pm\) 0.0066 & & & & & & * & * & & \\ N2M2 & 0.0346 \(\pm\) 0.0053 & & & & & & & & / & \\ N2M3 & 0.0425 \(\pm\) 0.0033 & & & & & & & & / & ** \\ N3M1 & 0.0338 \(\pm\) 0.0042 & & & & & & & & / & ** \\ N3M2 & 0.0349 \(\pm\) 0.004 & & & & & & & & & ** \\ N3M3 & 0.0465 \(\pm\) 0.0067 & & & & & & & & & \\ \hline \hline \end{tabular}
\end{table}
Table 4: Statistical analysis of 4-NP contents in liver at Day 15.  
Figure 1: Statistical analysis of the content variation of 4-nonylphenol (4-NP) in liver at Day 7, Day 15 and Day 30. N, 4-NP; M, montmorillonite.  
\begin{table}
\begin{tabular}{c c c c c c c c c c c} \hline \hline  & **4-NP Contents (ug/g)** & **N2** & **N3** & **NiM1** & **NiM2** & **NiM3** & **N2M1** & **N2M2** & **N2M3** & **N3M1** & **N3M2** & **N3M3** \\ \hline N1 & 0.0331 \(\pm\) 0.0038 & / & * & / & / & & * & * & & \\ N2 & 0.0318 \(\pm\) 0.0035 & & & & / & & * & * & & \\ N3 & 0.0408 \(\pm\) 0.0063 & & & & & & & / & / & / \\ N1M1 & 0.0357 \(\pm\) 0.0045 & & & / & / & & & / & / & \\ N1M2 & 0.0341 \(\pm\) 0.004 & & & / & / & & / & & / & * \\ N2M1 & 0.0354 \(\pm\) 0.0034 & & & & & / & / & & * \\ N2M2 & 0.0389 \(\pm\) 0.0062 & & & & & / & / & / & * \\ N2M3 & 0.0366 \(\pm\) 0.0040 & & & & & & & / & * \\ N3M1 & 0.0325 \(\pm\) 0.0077 & & & & & & & / & * \\ N3M2 & 0.0368 \(\pm\) 0.0036 & & & & & & & & * \\ N3M3 & 0.0436 \(\pm\) 0.0046 & & & & & & & & * \\ \hline \hline \end{tabular}
\end{table}
Table 5: Statistical analysis of 4-NP contents in liver at Day 30.