## 3 Results
### Quality Indicators of the Obtained Extracts  
The quality assessment of the _M. memrosum_ herb liquid extracts (MN40 and MN70) (three batches of the extracts) was carried out according to the methods of European pharmacopeia [16] and the following parameters (Table 2): identification A (description), identification B (main phenolic compounds by thin-layer chromatography (TLC) method), \begin{table}
\begin{tabular}{c c c c} \hline \hline \multicolumn{1}{c}{**No.**} & \multicolumn{1}{c}{**Compound**} & \multicolumn{2}{c}{**Content in the Liquid Extract, \(\mu\)g/mL**} \\  & & **MN40** & **MN70** \\ \hline \multicolumn{4}{c}{Phenolic acids} \\ \hline
1 & Hydroxyphenylacetic acid & 54.49 \(\pm\) 3.82 & 53.64 \(\pm\) 3.79 \\
2 & Benzoic acid & 1294.43 \(\pm\) 76.17 & 1274.69 \(\pm\) 99.35 \\ \hline \multicolumn{4}{c}{Hydrooxycinamic acids} \\ \hline
3 & Chlorogenic acid * & 203.66 \(\pm\) 8.05 & 218.74 \(\pm\) 14.79 \\
4 & Caffe acid * & 157.79 \(\pm\) 7.37 & 166.68 \(\pm\) 7.82 \\
5 & _p_-Coumaric acid * & 696.42 \(\pm\) 45.86 & 810.55 \(\pm\) 53.40 \\
6 & _trans_-Ferulic acid * & 25.66 \(\pm\) 2.18 & 32.14 \(\pm\) 2.69 \\
7 & _trans_-Cinnamic acid * & 399.40 \(\pm\) 18.97 & 524.36 \(\pm\) 31.04 \\ \hline \multicolumn{4}{c}{Flavonoids} \\ \hline
8 & Rutin * & 4625.30 \(\pm\) 183.32 & 5009.22 \(\pm\) 144.72 \\
9 & Quercetin-3-O-glucoside & 532.21 \(\pm\) 2.18 & 517.76 \(\pm\) 3.18 \\
10 & Naringin * & 927.24 \(\pm\) 40.13 & 1042.37 \(\pm\) 55.72 \\
11 & Neohseperidin * & 843.98 \(\pm\) 34.40 & 993.36 \(\pm\) 31.04 \\
12 & Quercetin * & 1217.03 \(\pm\) 45.12 & 1416.42 \(\pm\) 52.67 \\
13 & Naringenin * & 475.70 \(\pm\) 18.02 & 596.21 \(\pm\) 22.61 \\
14 & Apigenin * & 145.78 \(\pm\) 7.64 & 189.17 \(\pm\) 9.90 \\
15 & Rhamnetin * & 917.69 \(\pm\) 38.77 & 1026.85 \(\pm\) 43.38 \\
16 & Kaempferol * & 345.48 \(\pm\) 15.15 & 414.50 \(\pm\) 18.21 \\ \hline \multicolumn{4}{c}{Tannin metabolites} \\ \hline
17 & Catechin * & 266.99 \(\pm\) 14.06 & 217.39 \(\pm\) 11.49 \\
18 & Epicatechin & 567.57 \(\pm\) 28.53 & 548.80 \(\pm\) 27.74 \\
19 & Gallocatechin * & 165.71 \(\pm\) 16.11 & 192.83 \(\pm\) 20.04 \\ \hline \multicolumn{4}{c}{Content of compound groups in the liquid extract, \% (spectrophotometry)} \\ \hline Total polyphenols (\%) * & 4.73 \(\pm\) 0.20 & 6.77 \(\pm\) 0.21 \\ Flavonoids (\%) * & 0.86 \(\pm\) 0.07 & 1.01 \(\pm\) 0.06 \\ \hline \hline \end{tabular} Notes: MN40, extract obtained with 40% aqueous-ethanol solution; MN70, extract obtained with 70% aqueous-ethanol solution; _p_-values \(<\) 0.05 were considered statistically significant, while values above 0.05 indicated no significant difference between the groups. All calculations were based on independent two-sample t-tests. Asterisks (*) denote statistically significant differences in the content of substances between MN40 and MN70.  
\end{table} 
Table 1: Content of phenolic compounds in the liquid extracts of _Melampryum_ hemroosum_ herb. quantitative content of flavonoids (not less than 0.8% (for MN40) or 0.9% (for MN70)), heavy metals (not more than 0.002%) [16], dry residue (not less than 12.0%) [16], ethanol content (38-42 or 68-72%, respectively) [16], and microbiological purity (microbiological quality criteria: total aerobic microbial count (TAMC): acceptance criterion: \(\leq\)10\({}^{5}\) CFU/g or CFU/mL, maximum allowable limit: 500,000 CFU/g or CFU/mL; total yeast and mould count (TYMC): acceptance criterion: \(\leq\)10\({}^{4}\) CFU/g or CFU/mL, maximum allowable limit: 50,000 CFU/g or CFU/mL; bile-tolerant Gram-negative bacteria: acceptance criterion: \(\leq\)10\({}^{4}\) CFU/g or CFU/mL; _Escherichia coli_: absence in 1 g or 1 mL; _Salmonella_ spp. : absence in 25 g or 25 mL) [16]. TLC analysis of the extracts, conducted in comparison with authentic standards, confirmed the presence of hydroxycinnamic acids (caffeic and chlorogenic) and flavonoids (quercetin and rutin). The studied extracts of _M. memorosum_ herb met the specified numerical parameters and microbiological purity requirements outlined in the proposed draft quality control methods.