## 3 Results
### Formulated Conidia Shelf Life
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Treatments**} & \multicolumn{2}{c}{**Storage Period at 4 \(\pm\) 1 \({}^{\circ}\)C**} & \multicolumn{2}{c}{**Storage Period at 25 \(\pm\) 3 \({}^{\circ}\)C**} \\ \cline{2-6}  & **0 d** & **15 d** & **30 d** & **0 d** & **15 d** & **30 d** \\ \hline
0.1\% _Acacia_ & 92.96 \(\pm\) 0.26 a & 87.34 \(\pm\) 0.64 a & 87.77 \(\pm\) 1.1 a & 92.09 \(\pm\) 0.73 a & 87.99 \(\pm\) 0.69 a & 64.0 \(\pm\) 1.62 a \\
0.2\% _Acacia_ & 90.29 \(\pm\) 1.36 a & 86.26 \(\pm\) 0.84 a & 90.28 \(\pm\) 0.88 a & 91.43 \(\pm\) 1.57 a & 87.47 \(\pm\) 1.98 a & 61.36 \(\pm\) 2.57 a \\
0.25\% _Acacia_ & 90.56 \(\pm\) 0.99 a & 81.84 \(\pm\) 2.29 a & 85.96 \(\pm\) 1.29 a & 90.94 \(\pm\) 1.27 a & 86.49 \(\pm\) 1.16 a & 61.03 \(\pm\) 3.93 a \\
0.5\% _Acacia_ & 90.69 \(\pm\) 1.38 a & 90.15 \(\pm\) 1.25 a & 89.63 \(\pm\) 2.65 a & 92.86 \(\pm\) 0.87 a & 84.31 \(\pm\) 0.64 a & 67.43 \(\pm\) 2.59 a \\
0.7\% _Acacia_ & 90.44 \(\pm\) 1.51 a & 88.26 \(\pm\) 1.91 a & 90.68 \(\pm\) 0.85 a & 93.5 \(\pm\) 0.78 a & 88.62 \(\pm\) 1.08 a & 66.32 \(\pm\) 2.05 a \\
0.1\% _Hirstella_ & 92.47 \(\pm\) 1.16 a & 87.99 \(\pm\) 0.84 a & 80.30 \(\pm\) 0.85 a & 94.58 \(\pm\) 0.05 a & 81.93 \(\pm\) 0.9 a & 60.72 \(\pm\) 1.48 a \\
0.2\% _Hirstella_ & 94.29 \(\pm\) 0.32 a & 90.47 \(\pm\) 2.73 a & 86.40 \(\pm\) 2.14 a & 93.49 \(\pm\) 1.29 a & 83.12 \(\pm\) 1.74 a & 59.44 \(\pm\) 2.91 a \\
0.25\% _Hirstella_ & 92.5 \(\pm\) 0.88 a & 90.38 \(\pm\) 1.5 a & 86.91 \(\pm\) 2.07 a & 89.09 \(\pm\) 0.31 a & 86.99 \(\pm\) 1.24 a & 68.76 \(\pm\) 1.83 a \\
0.5\% _Hirstella_ & 93.17 \(\pm\) 1.13 a & 91.31 \(\pm\) 0.99 a & 93.20 \(\pm\) 0.8 a & 91.95 \(\pm\) 1.36 a & 87.86 \(\pm\) 1.65 a & 72.13 \(\pm\) 0.9 a \\ Control & 92.68 \(\pm\) 0.32 a & 74.28 \(\pm\) 1.63 b & 53.36 \(\pm\) 3.58 b & 91.59 \(\pm\) 0.73 a & 77.5 \(\pm\) 1.49 b & 31.34 \(\pm\) 3.47 b \\ \hline \hline \end{tabular}  
* Values followed by the same letter indicate no significant difference (Tukey \(\alpha\) = 0.05). Data represent means \(\pm\) SEM of triplicate determinations from three independent experiments.  
\end{table} 
Table 3: Effect of gum and temperature on _Hirstella ctirfornis_ OP-Hir-10 strain conidia germination after storage for up to 30 d 1. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & \multicolumn{5}{c}{**Germination (Mean \(\pm\) SEM)**} \\ \hline
**Days** & **FH3-1** & **FH3-2** & **FH3-3** & **FH3-4** & **FH3-Control** \\ \hline
0 & 95.5 \(\pm\) 0.41 a & 95.8 \(\pm\) 0.69 a & 96.2 \(\pm\) 0.53 a & 94.7 \(\pm\) 0.61 a & 91.8 \(\pm\) 0.86 b \\
30 & 94.0 \(\pm\) 0.37 a & 93.7 \(\pm\) 0.41 a & 92.8 \(\pm\) 0.86 ab & 92 \(\pm\) 0.53 b & 90.2 \(\pm\) 0.53 c \\
60 & 89.0 \(\pm\) 0.98 a & 87.7 \(\pm\) 2.24 a & 87.8 \(\pm\) 1.63 a & 87.0 \(\pm\) 1.67 a & 83.7 \(\pm\) 1.67 a \\
90 & 77.7 \(\pm\) 1.67 ab & 79.2 \(\pm\) 1.31 ab & 80.3 \(\pm\) 0.73 a & 76.0 \(\pm\)2.12 b & 68.3 \(\pm\) 1.22 c \\
120 & 61.3 \(\pm\) 3.34 a & 64.0 \(\pm\) 2.53 a & 67.5 \(\pm\) 1.63 a & 66.7 \(\pm\) 2.28 a_Hirstella citriformis_ conidia viability of OP-Hir-3 (Table 6) and OP-Hir-10 (Table 7) strains was similar among all formulation treatments, after storage at 4 \(\pm\) 1 \({}^{\circ}\)C. Only the control showed a significant (F\({}_{4,25}\) = 18.18; \(p\)\(\leq\) 0.05) and (F\({}_{4,25}\) = 118.2; \(p\)\(\leq\) 0.05) difference, respectively. At 120 d of storage, conidia viability of OP-Hir-3 and OP-Hir-10 strains decreased by 16.5% and 17.3%, respectively. Viability reduction of conidia control ranged from 23.16% to 34.3%. After evaluating if a higher temperature decreases conidia viability after 90 d of storage, results indicated a significantly lower viability of conidia in those formulations stored at higher temperatures. This was observed with OP-Hir-3 (Tabasco; Figure 1A) (F\({}_{4,11}\) = 3.356; \(p\)\(\leq\) 0.05) and OP-Hir-10 (Yucatan; Figure 1B) (F\({}_{4,11}\) = 3.356; \(p\)\(\leq\) 0.05) strains, showing that storage at 4 \(\pm\) 1 \({}^{\circ}\)C decreased the loss of viability of the active ingredient.