## 2 Results and Discussion
### BJ Human Normal Fibroblasts Cell Line  
Analyzing the effect of different cookies with the addition of selected wild berries on their cytotoxic properties against the human normal fibroblast BJ cell line, it was observed that higher concentrations (1.5-2.5 mg/mL) of extracts with the addition of wild berries were characterized by greater cytotoxic properties (Table 4). However, generally, cell viability of the BJ line was high, reaching more than 85% in most cases, with a few exceptions where it was 65-73% after the use of elder and hawthorn berries extracts at 2.5 mg/mL concentration (Table 4). When the viability of normal BJ fibroblasts was compared with the vitality of the two cancer cells, MCF7 and WM793, treated with the same extracts, the activity of the latter was in most cases statistically significantly lower (\(p<0.05\)), especially in the case of the MCF7 breast tumor cell line for all types of extracts (up to 40-50% lower for chokeberry and elderberry in the concentration of extracts). Sea buckthorn extracts greatly reduced the shelf life of the WM793 melanoma cell line (\(p<0.05\)). This may indicate that wild-grown berries have different anti-tumor health promoting properties, and their efficacy in this regard depends on the type of tumor cell line they act on (Figure 2).  
\begin{table}
\begin{tabular}{c c c c} \hline  & & **Cytotoxicity 1**  & **Viability 1**  \\ \hline \multirow{2}{*}{**Extract Type**} & **Extract Concentration** & & **Mean Value [\%] \(\pm\) Standard Deviation** \\  & **[mg/mL]** & & \\ \hline \multirow{4}{*}{\begin{tabular}{c} Cokeberry \\ _(Aronia melanocarpa)_ \\ \end{tabular} } & 0.5 & 1.93  \({}^{a}\) \(\pm\) 0.04 & 98.90  \({}^{b}\) \(\pm\) 0.00 \\  & 1.0 & 8.65  \({}^{a}\) \(\pm\) 0.07 & 100.00  \({}^{b}\) \(\pm\) 0.07 \\  & 1.5 & 31.20  \({}^{b}\) \(\pm\) 0.30 & 98.07  \({}^{b}\) \(\pm\) 0.10 \\  & 2.5 & 27.80  \({}^{b}\) \(\pm\) 0.02 & 88.67  \({}^{b}\) \(\pm\) 0.10 \\ \hline \multirow{4}{*}{\
\begin{tabular}{c} Hawthorn \\ _(Cratageus L-)_ \\ \end{tabular} } & 0.5 & 1.40  \({}^{a}\) \(\pm\) 0.01 & 91.94  \({}^{b}\) \(\pm\) 0.05 \\  & 1.0 & 7.98  \({}^{a}\) \(\pm\) 0.13 & 88.01  \({}^{b}\) \(\pm\) 0.10 \\  & 1.5 & 14.79  \({}^{a}\) \(\pm\) 0.16 & 88.94  \({}^{b}\) \(\pm\) 0.03 \\  & 2.5 & 47.05  \({}^{b}\) \(\pm\) 0.47 & 72.79  \({}^{c}\) \(\pm\) 0.14 \\ \hline \multirow{4}{*}{
\begin{tabular}{c} Happy \\ _(Hippophae rhamnoides_ L-)_ \\ \end{tabular} } & \(x\) & 8.86 & 80.99 \\ \hline \end{tabular}
\end{table}
Table 4: Cytotoxicity assay after 24 h incubation of different wild-grown fruits enriched cookies on BJ human normal fibroblasts.  
\begin{table}
\begin{tabular}{c c c} \hline  & **Cytotoxicity 1**  & **Viability 1**  \\ \hline \multirow{4}{*}{\begin{tabular}{c} Extract Type \\ _(_mg/mL_) \\ \end{tabular} } & **Extract Concentration** & & **Mean Value [\%] \(\pm\) Standard Deviation** \\  & **[mg/mL]** & & \\ \hline \multirow{4}{*}{\begin{tabular}{c} Cokeberry \\ _(Aronia melanocarpa)_ \\ \end{tabular} } & 0.5 & 0.0 & 39.74  \({}^{b}\) \(\pm\) 0.02 \\  & 1.0 & 0.0 & 39.34  \({}^{b}\) \(\pm\) 0.04 \\  & 1.5 & 0.0 & 35.76  \({}^{b}\) \(\pm\) 0.05 \\  & 2.5 & 0.0 & 37.60  \({}^{b}\) \(\pm\) 0.06 \\ \hline \multirow{4}{*}{\begin{tabular}{c} Control \\ _(_1_) \\ \end{tabular} } & \(x\) & - & 38.11 \\ \hline \multirow{4}{*}{\
\begin{tabular}{c} Control \\ _(_1_) \\ \end{tabular} } & 0.5 & 0.0 & 64.98  \({}^{d}\) \(\pm\) 0.08 \\  & 1.0 & 0.0 & 62.53  \({}^{d}\) \(\pm\) 0.11 \\  & 1.5 & 0.0 & 60.19  \({}^{d}\) \(\pm\) 0.11 \\  & 2.5 & 0.0 & 57.29  \({}^{d}\) \(\pm\) 0.10 \\ \hline \multirow{4}{*}{
\begin{tabular}{c} Control \\ _(_1_) \\ \end{tabular} } & \(x\) & - & 61.25 \\ \hline \end{tabular}
\end{table}
Table 3: _Cont._