## 2 Results
### Contents of Secondary Metabolites in Maturated Hop Cones of Healthy and HVLd-Infected Plants
Similarly, the contents of terpene epoxides in EO were increased in infected hop plants depending on the harvest year and the same applied vice versa, wherein contents of terpene ketones in EO were reduced in infected hop plants depending on the harvest year (Table 3). The situation inside the terpene esters group was variable if the contents of methylgeranate, methylheptanoate, methylnon-6-enoate, and methyldeca-4,8-dienoate were increased; however, the contents of methylocanoate, methyl-8-methylnonanoate, methyldecanoate, and methyldodeca-3,6-dienoate were reduced in infected hop plants (Table 3). The results of the EO analyses for cultivars Sladek, Preimiant, and Agnus are summarized in Table 4. The same trends as those in Saaz hop samples were not found and the contents of individual EO compounds non-significantly varied due to cultivar genome and environmental factors of the harvest years. The content of total essential oils was slightly reduced by vivoid infection for all the cultivars (Table 4). Only the contents of linalool, geraniol, and methylgeranate were similarly increased in infected hop plants depending on the harvest year (Table 4). \begin{table}
\begin{tabular}{c c c c c c c} \hline
**Cultivar** & \multicolumn{2}{c}{**Sladek**} & \multicolumn{2}{c}{**Premiant**} & \multicolumn{2}{c}{**Agnus**} \\ \hline
**HLVd infection** & **Negative** & **Positive** & **Negative** & **Positive** & **Negative** & **Positive** \\
**Number of samples** & **9** & **7** & **3** & **3** & **3** & **4** \\ \hline Alpha acids (\% of DW) & 9.31 \(\pm\) 0.98 & 6.68 \(\pm\) 0.17 *** & 8.86 \(\pm\) 0.30 & 7.55 \(\pm\) 0.34 *** & 11.53 \(\pm\) 0.45 & 10.52 \(\pm\) 0.27 *** \\ Beta acids (\% of DW) & 3.83 \(\pm\) 0.46 & 4.44 \(\pm\) 0.24 *** & 3.32 \(\pm\) 0.41 & 3.23 \(\pm\) 0.33 & 3.90 \(\pm\) 0.28 & 3.52 \(\pm\) 0.20 * \\ Chumolume (\% of AA) & 25.31 \(\pm\) 0.55 & 23.63 \(\pm\) 0.39 *** & 17.13 \(\pm\) 1.62 & 16.33 \(\pm\) 0.51 & 29.97 \(\pm\) 1.08 & 30.55 \(\pm\) 0.61 \\ Columbitone (\% of BA) & 49.21 \(\pm\) 0.82 & 49.29 \(\pm\) 0.47 & 37.30 \(\pm\) 3.32 & 38.23 \(\pm\) 0.12 & 52.80 \(\pm\) 1.31 & 53.50 \(\pm\) 0.57 \\ Xanthohumol (\% of DW) & 0.606 \(\pm\) 0.045 & 0.467 \(\pm\) 0.027 & 0.313 \(\pm\) 0.021 & 0.247 \(\pm\) 0.006 *** & 0.747 \(\pm\) 0.067 & 0.718 \(\pm\) 0.058 \\ \hline \end{tabular}  
* Probability level: *\(-\)\(p\) < 0.1, ***\(-\)\(p\) < 0.05, and ***\(-\)\(p\) < 0.01. Abbreviations: DW—dry weight; AA—alpha acids; and BA—beta acids.  
\end{table} 
Table 2: Hop bitter acids and xanthohumol contents (average \(\pm\) S\({}_{\text{D}}\)) in dry cones of HLVd-free and infected plants of the Sladek, Preimiant, and Agnus cultivars. Figure 1: Contents of hop bitter acids in dry cones of HLVd-free and infected plants of the (**A**) Saaz and (**B**) hybrid cultivars. Probability level: *—\(p\) < 0.1, ***\(-\)\(p\) < 0.05, and ***\(-\)\(p\) < 0.01. Note: straight line—median; dashed line—average; and box—95% percentile \(\pm\) standard deviation. Yields of dry hop cones were measured only for healthy and HLVd-infected plants of cultivar Saaz. The yield was significantly reduced for both years by 19.3% and 18%, respectively, in infected hop plants. The average yields were 0.659 \(\pm\) 0.194 ** kg of DW per healthy plant and 0.532 \(\pm\) 0.218 kg of DW per infected plant in 2019, and 0.672 \(\pm\) 0.231 ** kg of DW per healthy plant and 0.551 \(\pm\) 0.227 kg of DW per infected plant in 2020.