## 3 Results
### Soil Microbial Community Structure Based on FAME Analysis
\begin{table}
\begin{tabular}{c c c c c c c c c c} \hline
**Year** & **Treatment** & **F.B Ratio** & **G+/G– Ratio** & **AMF/SF Ratio** & **AMF/SF Ratio** & **SF/TB Ratio** & **c/type Ratio** & **Shannon Index** \\ \hline \multirow{4}{*}{2018} & Control & 0.64 (0.07) & 3.73 (0.11) & 0.15 (0.03) & 0.08 (0.01) & 0.57 (0.07) & 0.58 (0.01)a & 131.54 (35.45) \\  & 5\%-Sur & 0.66 (0.11) & 4.21 (0.27) & 0.17 (0.03) & 0.09 (0.01) & 0.58 (0.1) & 0.49 (0.04)b & 130.59 (0.26) \\  & 5\%-Inc & 0.54 (0.00) & 4.07 (0.02) & 0.17 (0.01) & 0.08 (0.01) & 0.46 (0.03) & 0.47 (0.01b) & 110.41 (0.86) \\  & 10\%-Inc & 0.64 (0.04) & 3.9 (0.09) & 0.17 (0.02) & 0.09 (0.01) & 0.55 (0.05) & 0.47 (0.01b) & 154.20 (31.00) \\  & Control & 0.76 (0.1) & 3.84 (0.17) & 0.15 (0.01) & 0.09 (0.01) & 0.66 (0.04) & 0.48 (0.02) & 134.01 (68.39) \\  & 5\%-Sur & 0.78 (0.03) & 3.77 (0.21) & 0.18 (0.02) & 0.11 (0.01) & 0.66 (0.04) & 0.41 (0.02b) & 229.88 (43.62) \\  & 5\%-Inc & 0.74 (0.05) & 4.07 (0.29) & 0.21 (0.02) & 0.13 (0.01) & 0.61 (0.05) & 0.41 (0.01c) & 154.95 (55.31) \\  & 10\%-Inc & 0.67 (0.07) & 3.69 (0.12) & 0.21 (0.04) & 0.11 (0.02) & 0.55 (0.06) & 0.41 (0.01c) & 175.93 (81.82) \\  & Treat & n.s. & n.s. & n.s. & n.s. & n.s. & 0.001 & n.s. \\ ANOVA & Year & 0.022 & n.s. & n.s. & 0.002 & n.s. & 0.001 & 0.001 \\  & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} \\  & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} \\  & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} \\  
2019 & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{}{} & \multicolumn{1}{c}{}{} & \multicolumn{1}{c}{}{} & \multicolumn{1}{c}{}{} & \multicolumn{1}{c}{}{} & \multicolumn{1}{c}{}{} & \multicolumn{1}{c}{}{} & \multicolumn{1}{c}{}{} & \multicolumn{1}{c}{}{} & \multicolumn{1}{c}{}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{}{} & \multicolumn{1}{c}{}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{}{} &Principal component analysis (PCA) was conducted using the individual FAMEs (mol %) that were detected in all the treatments (Figure 1). At soil depth of 0-15 cm, principal component 1 (PC1) and principal component 2 (PC2) accounted for 51.3 and 16.0% of the total variation, respectively (Figure 1a). When plotted, PCA illustrated clear separations between the sample years. The 2018 samples clustered with negative values of PC1 samples, and the 2019 biochar additions samples were grouped with the same component's positive values. PC2 values for 5%-Sur soils were greater compared to both incorporated (Inc) soil treatments. Principal component 1 (PC1) and principal component 2 (PC2) accounted for 51.4% and 17.3% of the total variation respectively at the 15-30 cm depth. Like the 0-15 cm 2019 samples, the 15-30 cm depth clustered positively with PC1, but in the 2018 sample, the control and 10%-Inc gathered and have smaller PC2 values than 5%-Inc and 5%-Sur (Figure 1b).