## 3 Results and Discussion
### Antibacterial Activity
aureus_ was observed when LS, PS, and RS were applied (Table 3); BS recorded no inhibition effect, regardless of the concentration used. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & \begin{tabular}{c} **Concentration** \\ **(\(\mu\)L)** \\ \end{tabular} & _E. coli_ & _P. mirabilis_ & _K. pneumoniae_ & _E. faecalis_ & _S. aureus_ \\ \hline \multirow{4}{*}{BS} & 2 & - & - & - & - \\  & 50 & - & - & - & - \\  & 70 & - & - & - & - \\  & 100 & - & - & - & - \\ \hline \multirow{4}{*}{PS} & 2 & - & - & - & 11 \\  & 50 & - & - & - & 24 \\  & 70 & - & - & - & 25 \\  & 100 & - & - & - & 25 \\ \hline \multirow{4}{*}{LS} & 2 & - & - & - & - \\  & 50 & - & - & - & - \\  & 70 & - & - & - & 25 \\  & 100 & - & - & - & - & 26 \\ \hline \multirow{4}{*}{RS} & 2 & - & - & - & 12 & 13 \\  & 50 & - & - & - & 12 & 15 \\  & 70 & - & - & - & 12 & 25 \\  & 100 & - & - & - & 14 & 25 \\ \hline \begin{tabular}{c} Negative \\ control \\ \end{tabular} & - & - & - & - & - & - \\ \hline \multirow{4}{*}{\begin{tabular}{c} Positive \\ control \\ \end{tabular} } & 30 for lev- & 25 for & \begin{tabular}{c} 17 for \\ levofloxacin \\ \end{tabular} 17 & 16 for & 33 for \\  & \begin{tabular}{c} \\ for ciprofloxacin \\ \end{tabular} & \
\begin{tabular}{c} vancomycin \\ \end{tabular} &
\begin{tabular}{c} linez solid \\ \end{tabular} \\ \hline \hline \end{tabular}  
PS—pea sprouts, RS—radish sprouts, BS—bean sprouts, LS—lentil sprouts. The values are expressed as mean values of three measurements.  
\end{table} 
Table 3: The diameter of inhibition zone (mm) for the analyzed sprouts at different extract concentrations. The MIC values for the analyzed extracts, in relation to the tested microorganisms, are as follows. PS--70 \(\mu\)L/mL and RS--50 \(\mu\)L/mL for _Staphylococcus aureus_ and, for _Enterococcus faecalis_, RS--70 \(\mu\)L/mL. In the case of the other extracts, they are without effect for the analyzed microbial strains at the volume of the analyzed extract. Regarding the size of the halos that indicate the sensitivity to antibiotics of various microorganisms, the antibiotic substances that generate halos (including the diameter of the micro-tablet) larger than 14 mm are considered effective, intermediately sensitive are those between 12 and 14 mm, and resistant are those that generate halos smaller than 12 mm (for Enterobacteriaceae family). In the case of the _Staphylococcus aureus_ species, if we take linez solid as a standard, for a halo size greater than 24 mm, the strain is sensitive (S); between 21 and 24 mm, it is considered intermediate sensitive (IS); and below 21 mm is resistant (R), according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) [39]. In the case of the species _Enterococcus faecalis_ (vancomycin as standard), for a halo size greater than 13 mm, the strain is sensitive (S); between 10 and 13 mm, it is considered intermediate sensitive (IS); and below 10 mm, it is resistant (R), according to EUCAST [39]. Based on these findings, we can say that the _S. aureus_ strain is sensitive to the application of a concentration of 70 and 100 \(\mu\)L of PS, RS, and LS extract, with a halo of 25 mm and intermediate sensitivity (IS) for a concentration of 50 \(\mu\)L PS extract. Against _Enterococcus faecalis_, when 100 \(\mu\)L RS extract was applied and a halo of 14 mm was observed, it meant the strain was sensitive (S) at this concentration of RS extract. For lower concentrations, the strain is of intermediate sensitivity (IS) upon the application of RS extract  
Figure 7 shows the percentage of inhibition of the analyzed extracts at the maximum concentration applied (100 \(\mu\)L/mL, against the two sensitive strains (_S. aureus_ and _E. faecalis_) and shows the relation to the effect produced by the positive control antibiotic for which the maximum inhibition is considered. A high percentage of _S. aureus_ inhibition of over 75% is observed with the application of three of the total four analyzed sprouts (PS, LS, RS) and an 87.5% inhibition efficacy against _E.