## 2 Classes of Secondary Metabolites
### Polyhydroxaysterol and Steroids  
One polyhydroxaystrol, 23,24-dimethylcholest-16(17)-\(E\)-en-3\(\beta\),5\(\alpha\),6\(\beta\),20(\(S\))-tetrarol **374**, along with 24-methylcholestane-3\(\beta\),5\(\alpha\),6\(\beta\),25-tetrarol-25-monoacetate **375** and gorosten-5(\(E\))-3\(\beta\)-ol **376**, were reported from _S. trochelophorum_. Interpretation using 1 and 2D NMR analysis pointed out the existence of 23,24-dimethyl cholesterol derivatives which were further approved by the mass fragmentation pattern [29]. The isolation of (24\(S\))-24 methylcholestane-3\(\beta\),5\(\alpha\),6\(\beta\)-triol **377** from _S. crassocaule_ were also reported [28]. Sardiosterol **378** was isolated from _S. digitatum_ [PERSON]. The carbon NMR matched that of (22\(R\))-methylcholest-5-en-3\(\beta\), 22,25,28-tetrarol-3,22,28-triacetate **379** indicating that sardiosterol **378** has the same steroidal nucleus as (22\(R\)) -methylcholest-5-en-3\(\beta\), 22,25,28-tetrarol-3,22,28-triacetate **378** but the OH groups in carbon 22 and 28 were replaced by acetoxy groups [113]. (245)-24-methylcholestane-3\(\beta\),5\(\alpha\),6\(\beta\),25\(\gamma\),26-pentol-25,26-diacetate **380** and (245)-24-methylcholestane-3\(\beta\),5\(\alpha\),6\(\beta\),25\(\gamma\),26-pentol-26-\(n\)-decanoate **381**, was isolated from _S. trochelophorum_, while, (245)-24-methylcholestane-3\(\beta\),5\(\alpha\),6\(\beta\),25\(\gamma\)-tetrol **382** and (245)-24- methylcholestane-3\(\beta\),5\(\alpha\),6\(\beta\),25\(\gamma\) pentol-25-monoacetate **383** were reported from _S. glaucum_[114]. Fourteen polyoxygenated steroids with 3\(\beta\),5\(\alpha\),6\(\beta\)-hydroxy group, showing ergostane, cholestane, gorogostane and 23,24-dimethyl cholestane carbon skeletons were reported from _Saropython_ sp., 11\(\alpha\)-acetoxy-cholesta-24-en-3\(\beta\),5\(\alpha\),6\(\beta\)-triol **384**, (22\(E\),245)-11\(\alpha\)-acetoxy-ergostane-22,25-dien-3\(\beta\),5\(\alpha\),6\(\beta\)-triol **385**, (245)-ergostane-1\(\alpha\),3\(\beta\),5\(\alpha\),6\(\beta\),11\(\alpha\)-pental **386**, (245)-23,24-dimethylcholesta-22-en-3\(\beta\),5\(\alpha\),6\(\beta\),11\(\alpha\)-tetrarol **387**, (23\(R\),24\(R\))-23,24-dimethylcholesta-17(20) -en-3\(\beta\),5\(\alpha\),6\(\beta\)-triol **388**, 11\(\alpha\)-acetoxy-gorogostane-38,5\(\alpha\),6\(\beta\),12\(\alpha\)-tetrarol **389** and 12\(\alpha\)-acetoxy-gorogostane-38,5\(\alpha\),6\(\beta\),11\(\alpha\)-tetrarol **390**, sarcoaldosterolrol A **391**, (245)-ergostane-3\(\beta\),5\(\alpha\),6\(\beta\)-triol **392**, (245)-ergostane-3\(\beta\),5\(\alpha\),6\(\beta\),11\(\alpha\)-tetrarol **393**, (245)-ergostane-7-en-3\(\beta\),5\(\alpha\),6\(\beta\)-triol **394**, 11\(\alpha\)-acetoxy-gorostane-3\(\beta\),5\(\alpha\),6\(\beta\)-triol **395**, sarcoaldosterolrol B **396** and gorogostane-1\(\alpha\),3\(\beta\),5\(\alpha\),6\(\beta\),11\(\alpha\)-pental **397**. Structural elucidation for all isolates were done based on spectral analysis and comparing with reported literature [115]. Six polyhydroxy steroids, (245)-ergostan-3\(\beta\),5\(\alpha\),6\(\beta\),25-tetrarol-25-monoacetate **398**, (245)-24-methylcholestan-3\(\beta\),6\(\beta\),25-triol-25-O-acetate **399**, (245)-methylcholestan-3\(\beta\),5\(\alpha\),6\(\beta\),25-tetrarol-3,25-diacetate **400**, (245)-24-methylcholestan-1\(\beta\),3\(\beta\),5\(\alpha\),6\(\beta\),25-pentalool-25-monoacetate **401** and (245)-methylcholestan-3\(\beta\),5\(\alpha\),6\(\beta\),12\(\beta\),25-pentalool-25-O-acetate **402**, were reported from _Saropython_ sp., one was reported as 18-oxygenated polyhydroxy steroid, (245)-ergostan-3\(\beta\),5\(\alpha\),6\(\beta\),18,25-pentalool 18,25-diacetate **403**. The structure of this compound was determined through spectroscopic data, and its absolute configuration was elucidated by the modified Mosher's assay [116]. Chemical investigation of the polar fraction of _S. trochelophorum_, yielded two poly-hydroxy steroids, identified through extensive spectral analysis as zahramycins A and B **404** and **405**.