## 2 Results
### Ultra-High Sensitivity Quantitative Phosphoproteomic Analysis
\begin{table}
\begin{tabular}{c c c c} \hline ES8L1 & SLNSTPPPPPAPPAPPPPALAR & T5:Phospho & 0.031333883 \\ FIBA & PGSTGTWNFGSSER & S11:Phospho & 0.003771194 \\ GSK3B & GEPNVSYICSR & Y7:Phospho & 0.028989048 \\ GPC5B & TAGFPNGSLGK & S8:Phospho & 0.022155551 \\ GPC5C & ATANSQVMGSANSTLR & S13:Phospho & 0.010074399 \\ K1C13 & MIGFPSSAGSVSPR & S12:Phospho & 0.003025495 \\ KIF12 & SPGQVLPPH & S1:Phospho & \(1.37\times 10^{-10}\) \\ MAP1A & TLPQEPGK & T1:Phospho & 0.00176674 \\ MILK1 & SPVPSPSGSSSPQLQVK & S10:Phospho & 0.022496032 \\ MY15B & AVPSPPPPPIVK & S4:Phospho & 0.001997071 \\ NAT8L & MSVDSRFR & S5:Phospho & 0.029409743 \\ NPT2A & LGSPAVSPLPVR & S7:Phospho & 0.002418861 \\ PDZ11 & YSSMAASFR & S7:Phospho & 0.004247949 \\ SHAN2 & RAPSPVVSPTEMNK & S4:Phospho & 0.035395595 \\ VINEX & AETRLPSPK & T4:Phospho & 0.003764395 \\ VP37D & SAQPATSAADPPK & S1:Phospho & 0.023114006 \\ YG015 & IELSYNK & Y5:Phospho & 0.002020309 \\ ZNF25 & SHFIIIQR & S1:Phospho & 0.003312002 \\ \hline \end{tabular} \
\begin{tabular}{c c c} \hline AQP2 & RQSVELHSPSQLPR & S3:Phospho & 0.001562435 \\ AQP2 & RRQSVELHSPSQLPR & S4:Phospho & 0.001865546 \\ CAN7L & INSAHGSDKSK & S3:Phospho & 0.005547201 \\ CF170 & KWMHAHYSR & S8:Phospho & \(8.55\times 10^{-6}\) \\ CHM4C & LPNVPSSSLPAPQPNR & S8:Phospho & 0.03478666 \\ CI169 & NYAHISIPR & S7:Phospho & 0.014805139 \\ CK052 & TLKKPQPQQLQNLPK & T1:Phospho & 0.015894205 \\ CP250 & QSESLSELITLR & S2:Phospho & 0.007130084 \\ CSPP1 & QPSPIVPALQNK & S3:Phospho & 0.000997041 \\ EPS8 & APAPAPPGYTVQVDR & T9:Phospho & 0.001709885 \\ EPS8 & KGPGEGVITLR & T9:Phospho & 0.003312002 \\ ES8L2 & HSPTSEPTTPGDALPPVSSPHTH & S19:Phospho & 0.041726109 \\ EZRI & QLLITSSELSQAR & T4:Phospho & \(3.66\times 10^{-6}\) \\ GPC5C & VPSEGAYDILPR & S3:Phospho & 0.00052554 \\ ICK & STPGLIPRPPAAQPVHGR & T2:Phospho & 0.021340709 \\ IF4E2 & TASDQATTARIR & S3:Phospho & 0.019186167 \\ LDHA & TPKIVSGK & T1:Phospho & 0.020570378 \\ LMNA & SGAQASSTPLSPTRITR & S11:Phospho & 0.00927998 \\ MUC1 & DTYPHYSEPTYTHTHGR & S7:Phospho & 0.035395595 \\ RAB10 & FHITITSYYR & T3:Phospho & 0.006517696 \\ RAI3 & AHAWPSPYKDYEVK & S6:Phospho & 0.01798913 \\ SARG & ANSALITPKPESGLTLQESNTPGLR & T6:Phospho & 0.019581944 \\ SH56L & TPNLDWR & T1:Phospho & 0.000684591 \\ TRPV5 & ASALLPTSLSR & S11:Phospho & 0.00176674 \\ \hline \end{tabular}
\begin{tabular}{c c c} \hline Gene & Peptide & Phosphor\_Site & \(p\)-value \\ AQP2 & RQSVELHSPSQLPR & S3:Phospho & 0.00351656 \\ AQP2 & RRQSVELHSPSQLPR & S4:Phospho & 0.003246139 \\ CAN7L & INSAHGSDKSK & S3:Phospho & 0.007011764 \\ \hline \end{tabular}
\end{table} 
Table 2: _Cont._
A total of 2652 proteins were identified, of which 74% were also identified within the phosphoproteome and 26% were detected solely in the phosphorylation data, indicating that phosphoproteins are of considerably low abundance and may escape detection using the shotgun proteomics approach. The quantitative proteome analysis of uEVs demonstrated similar expression of most proteins in patients with DN and DM, whereas phosphopeptide levels were significantly different between patients with DN and those with diabetes. These findings suggest that the phosphorylation differences between patients