## 2 Materials and Methods
### Microorganisms and Cultivation Condition  
Cells of the phototrophic microorganisms _Chloella vulgaris_ C-1, _Arthospira (Spirulina) platensis_ (Nordst.) _Geitl. resnusu_ 1/02 and _Nostoc_ sp. _resnus_ Nss-14/11 were obtained from the IBCP RAS collection and the IPPAS collection of microalgae and cyanobacteria (Moscow, Russia). Bacterial cells of _Rhodococcus erythropolis_ AC-1514D and _Pseudomonas esterophilus_ V-1436D were obtained from the All-Russian Collection of Microorganisms (VKM, Moscow, Russia). The bacterial culture _Photobacterium phosphoreum_ B-1717 was obtained from the Russian National Collection of Industrial Microorganisms (Moscow, Russia).  
The preparation of the immobilized inoculums of _C. vulgaris_ cells with and without combination into a bacterial consortium was carried out according to the methods described earlier [13, 42]. Briefly, cells separated from the medium were suspended in a solution of poly(vinyl alcohol) (type16/1, 84 kDa, Sinopec Corp., Beijing, China). Then, this suspension was maintained at \(-70\)\({}^{\circ}\)C using a DS 78 compact freezer (Dairei Asia Sdn. Bhd, Kuala Lumpur, Malaysia) for 3 days. Then, it was slowly defrosted via the following two stages: the first one, at \(-20\)\({}^{\circ}\)C using a GN 3613 freezer (Liebherr, Biberach, Germany) for 3 h, and the second one, at \(8\)\(\pm\)\(2\)\({}^{\circ}\)C in a 2201 Combicoldrac II refrigerator (LKB Instruments Haglund, Saffle, Sweden). The obtained samples of cells immobilized by inclusion into a polymer cryogel formed at subzero temperature were used in the further experiments.  
Standard media (Tamiya medium, BG-11 medium, Zarrouk medium) [4] were used correspondingly for biomass accumulation and cultivation of suspended microalgae (_Chloella vulgaris_) and cyanobacteria cells (_Nostoc_ sp. and _Arthospira (spirulina) platensis_) when the cell suspensions were used as inoculums. Among investigated variants of media, one of them was used with the addition of 1 g/L glycerol.  
Horticultural water from a gardening facility (Sovkhoz dekorativnogo sadovodstva, Moscow, Russia) was used for cultivation of immobilized inoculums of _C. vulgaris_ cells and bacterial consortia. Wastewater with known characteristics [13] was applied in the experiments. It contained 0.99 g COD/L, where COD (chemical oxygen demands) was determined by the standard dichromatic method [43]. The main chemical content of the wastewater was as follows: lipids--0.17 g/L; proteins--0.13 g/L; and carbohydrates 0.27 g/L, at a pH of 6.5.  
The PFC additives in the growth medium were 0.5 or 1% (\(v/v\)). The initial concentration of methyl parathion was 0.15 mM in experiments with artificial consortia.  
Cultivation of all microorganisms was carried out in an experimental laboratory-scale closed-type photobioreactor under the conditions described previously [44]. To implement cultivation in a circulating regime for 3 days, cells of all phototrophic microorganisms were loaded into a photob bioreactor with a 20 L working volume, into which a medium was pumped at a flowrate of 20 L/day through a system of glass tubes with an inner diameter of 35 mm and an outer diameter of 38 mm by means of a centrifugal pump. Carbon dioxide (99.8% \(v/v\)) was bubbled through the tubes around the clock through a silicone membrane at a rate of 1-2 mL/min. Also in this system, constant illumination of 5000 lux was provided by light sources with wavelengths in the intervals of 450-480 nm and 640-700 nm simultaneously. The photob bioreactor was equipped with a fully automated system for monitoring the pH (7.2 \(\pm\) 0.2) of the medium and controlling the cultivation temperature (22 \(\pm\) 1 \({}^{\circ}\)C).