## 3 Results and Discussion  
Figures 2-4 illustrate typical chromatograms of the sample solutions. The Ax and Cx amounts accumulated in the liver and female and male gonads for all of the fish groups are presented in Table 2. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Group No**} & \multicolumn{2}{c}{**Female Gonads**} & \multicolumn{2}{c}{**Male Gonads**} & \multicolumn{2}{c}{**Liver**} \\ \cline{2-7}  & **Ax** & **Cx** & **Ax** & **Cx** & **Ax** & **Cx** \\ \hline
1 & 0.319 \(\pm\) 0.036 * & 0.207 \(\pm\) 0.015 * & 0.211 \(\pm\) 0.009 * & 0.099 \(\pm\) 0.006 * & 0.176 \(\pm\) 0.007 * & 0.103 \(\pm\) 0.010 * \\ \hline
2 & 2.490 \(\pm\) 0.247 * & 2.206 \(\pm\) 0.166 * & 1.215 \(\pm\) 0.010 * & 0.888 \(\pm\) 0.010 * & 0.257 \(\pm\) 0.007 * & 0.156 \(\pm\) 0.006 * \\ \hline
3 & 0.518 \(\pm\) 0.020 * & 0.335 \(\pm\) 0.019 * & 0.395 \(\pm\) 0.012 * & 0.210 \(\pm\) 0.010 * & 0.351 \(\pm\) 0.008 * & 0.308 \(\pm\) 0.009 * \\ \hline \hline \end{tabular}  
* The content of Ax and Cx is expressed as mg/kg; different letters in the table denote significant differences between the experimental groups and control values according to LSD test (\(p\leq 0.05\)).  
\end{table} 
Table 2: Ax and Cx amounts in different organs of rainbow trout, _O. mykiss_ (n = 6, mean \(\pm\) SD). Figure 1: Typical chromatogram of standard solution. Many research teams explored the influence of dithiocarbamates including MZ on fish and their health. An investigation of the effect of MZ on biochemical and enzymatic parameters in _Clarius batrachus_ ([PERSON], 1758) in different fish life stages was carried out, and the authors concluded they were dose- and time-dependent [27,28]. MZ can cause genotoxic effects [29] and changes in the physiology of the liver and muscle tissues of catfish [30]. The authors concluded that fingerlings were more sensitive to MZ than adults. The dose of 1.1 mg/L (1/2 LC50 of MZ) caused important changes in the hematological properties of rainbow trout [31]. However, data concerning long-term exposure to MZ and the resulting impact on fish health are scarce. According to the official EPA information, the 72 h exposure of rainbow trout (_O. mykiss_) to MZ at concentration levels higher than 0.91 mg/L is categorized as highly toxic [32]. In this study, a 60-day trial with rainbow trout _O. mykiss_ reared in water containing the fungicide MZ in the concentration level permitted by the European legislation was carried out. In a 60-day-trial-period experiment, a Ginko-biloba-extract-supplemented diet was applied to neutralize the negative effect  
Figure 3: Typical chromatogram of sample solution of male gonads. Figure 2: Typical chromatogram of sample solution of female gonads. of organophosphorus pesticide on rainbow trout [33]. The biochemical parameters measured were very encouraging, but specific amounts of the antioxidant supplement were included. In our study, to neutralize the negative effect of this long-term exposure, the fish were fed with a xanthophyll-supplemented diet. Ax and Cx were selected due to their known antioxidant activity [34]. Salmonids are able to adsorb them from their diet, and a carotenoid-supplemented diet is used in the fish farming industry. So, many researchers studied the accumulation rate of the pigment in fish products. An experiment on the effect of carotenoids, including liposomes as a diet supplement, on their accumulation in the flesh of rainbow trout was carried out during a trial period of three months [35]. Our team conducted a similar experiment and concluded that the accumulation of Ax and Cx in the muscle tissues of rainbow trout was most intensive up to the 60 th day of the xanthophyll-supplemented diet [36]. Because of the great importance of the age and size of the fish specimens for optimal pigment accumulation [37], 36-month-old males and females with body weights of 912 \(\pm\) 45 g were selected.