## 3 Results  
### Chemical Analysis  
All samples were collected from different locations in Poland. The five species of plant are represented by 38 samples that were extracted from 10 g of plant-dried materials and analyzed using UPLC to identify the profile of polyphenols and their concentrations (mg/g), followed by antimicrobial analysis to determine the minimum inhibitor concentration (MIC). Figure 1A,B show the mean bioactive compound values, standard deviation, and minimum and maximum values of _Plantago major_ L. in eight locations. Gallic acid, 4-hydroxybenzoic acid, vanillic acid, caffeic acid, syringic acid, vanillin, p-cumaric acid, benzoic acid, ferulic acid, sinapic acid, t-cinnamic acid, chlorogenic acid, protocatechuric acid, rosmarinic acid, salicylic acid, apigenin, catechchin, kaempfer, luteolin, naringenin, quercetin, and rutin are listed in Figure 1A,B. The average amount of gallic acid in _Plantago major_ L. is 0.16 \(\pm\) 0.08. Based on Figure 1A,B, vanillic acid ranges from 0.01 to 0.08. We have observed that rutin 9.73 \(\pm\) 0.92, t-cinnamic acid, chlorogenic acid, and syringe acid are _Plantago major_ L.'s most abundant bioactive compounds. Protocatechuric acid and salicylic acid are not present in the plant. While rosmarinic acid has the lowest mean value at 0.03, vanillic acid has a low standard deviation, and syringe acid has the highest one at 2.28. Figure 1B suggests that we have examined that the concentration of Total Phenolic Acid Content (TPC) is slightly higher than the Total Flavonoid Content (TFC) of the _Plantago major_ L samples. These findings imply that, in comparison to the other plants studied, _Plantago major_ L. has a moderate content of TPC and TFC. Although the standard deviations show some variation in the content between the samples, the total amount of flavonoids variation is relatively small. When compared to _Armoracia rusticana_ and _Hypericum perforatum_ L., _Plantago major_ L. has a relatively low mean of total phenolic compounds and TFC. Figure 1A,B show the mean bioactive compound values of _Armoracia rusticana_ from nine locations, in addition to the standard deviation and minimum and maximum values. The list shows bioactive compounds like gallic, caffeic, syringic, and sinapic acids, as well as flavonoids like luteolin and quercetin. Each location calculates the compound's mean values. Rutin had the highest mean values of the compounds in Figure 1A,B, ranging from 7.77 to 11.67 across locations. Syringic acid had the second-highest mean value, and caffeic acid, sinapic acid, t-cinnamic acid, and vanillin had a high mean concentration. Protocatechuic, rosmarinic, and salicylic acids were found in low concentrations. Protocatechuic and salicylic acids were absent in some places. Rutin, caffeic acid, t-cinnamic acid, and sinapic acid are _Armoracia Rusticana_'s most abundant bioactive compounds, according to these figures. Vanillin has a low standard deviation, while luteolin has a higher one. Some compounds had different mean values in different locations. These differences in mean values suggest that bioactive compound composition may depend on its geographical origin. Figure 1: _Cont._  
Figure 2 suggests that we have examined the TPC and TFC of samples of _Armoracia rusticana_. These findings imply that the TPC and TFC of _Armoracia rusticana_ are relatively high. However, the high total flavonoid standard deviation indicates that there is a lot of variation in the flavonoid content between the samples when interpreting the findings and coming to conclusions about the samples' bioactivity. Figure 1A,B show the mean concentrations (in mg/g extract), standard deviation, and minimum and maximum values of various phenolic compounds in _Hypericum perforatum_ (St. John's Wort) plants collected from 10 different locations. In general, the mean concentrations of phenolic compounds in the _Hypericum perforatum_ plants varied across the different locations.