## Materials and Methods
### Assessment of Algal Culture Growth, Nutrient Removal, and Biochemical Composition of Biomass  
The optical density of the culture was determined using a Lambda 35 spectrophotometer ([PERSON], Singapore) at 750 nm. The number of cells was counted using a counting chamber.  
The specific growth rate and biomass productivity were calculated using the equations reported by [PERSON] et al. [40]. The specific growth rate of microcalga (day\({}^{-1}\)) was calculated during the exponential phase of the growth. Biomass productivity (g L\({}^{-1}\) day\({}^{-1}\)) was calculated by dividing the final biomass yield (dry matter) by the total number of days within the experiment required to reach the stationary phase.  
The content of chlorophylls (\(a\), \(b\)) and carotenoids (mg L\({}^{-1}\)) was determined by the method using dimethyl sulfoxide as a solvent, as described previously [33; 38]. Briefly, 1 mL of microalgal culture was centrifuged at 10,000\(\times\)\(g\) for 5 min, after which the supernatant was discarded. After that, 1 mL of preheated dimethyl sulfoxide was added to the precipitate, resuspended, incubated for 5 min in a thermoshaker TS-100C (BioSan, Riga, Latvia) at 60 \({}^{\circ}\)C, and then centrifuged at 10,000\(\times\)\(g\) for 5 min. Absorption was measured at 480, 649, and 665 nm using a Lambda 35 spectrophotometer (Perkin Elmer, Singapore). The chlorophyll and carotenoid concentrations were calculated using the equation reported by [PERSON] [41].  
A commercial Bio-Rad protein assay kit (Munich, Germany) was used to determine the content of proteins as described previously [38]. Briefly, approximately 20 mg of dried  
\begin{table}
\begin{tabular}{c c c c c} \hline
**Treatment** & **Photoperiod** & **Instantaneous PPFD** & **Daily PPFD** & **Initial CO2** \\  & **(h)** & **(\(\mu\)mol m\({}^{-2}\) s\({}^{-1}\))** & **(mol m\({}^{-2}\) day\({}^{-1}\))** & **Concentration (\%)** \\ \hline PL1 & 16 & 650 & 37.44 & 2.0 \\ PL2 & 16 & 813 & 46.83 & 2.0 \\ PL3 & 16 & 975 & 56.16 & 2.0 \\ PL4 & 16 & 1138 & 65.55 & 2.0 \\ PL5 & 16 & 1300 & 74.88 & 2.0 \\ CL1 & 24 & 650 & 56.16 & 2.0 \\ CL2 & 24 & 1300 & 112.32 & 1.0 \\ CL3 & 24 & 1300 & 112.32 & 2.0 \\ CL4 & 24 & 1300 & 112.32 & 4.0 \\ \hline \end{tabular} Abbreviations are used to denote various treatments, which stand for periodic light (PL) and continuous light (CL).  
\end{table}
Table 1: Experimental design of photoautotrophic cultivation of _Scenedesmacae_ strain EZ-B1.  
algal biomass was combined with 1 mL of 0.5 M NaOH in a 2 mL metal lysing tube (MP Biomedicals, Illkrich, France) with two types of beads (0.6 g of 0.1 mm zirconium/silica beads and 0.4 g of 1.0 mm glass beads) and then homogenized for two 30 s pulses at speed level 6.0 m s\({}^{-1}\) with a 5 min cooling period between pulses. The extracted samples were incubated at 500 rpm in a thermoshaker TS-100C (BioSan, Riga, Latvia) at 60 \({}^{\circ}\)C. The mixtures were then transferred to new, clean tubes, and after centrifugation at 2000\(\times\)\(g\) for 5 min, the supernatants were used for protein measurement.  
The degree of ammonium nitrogen removal was assessed by the colorimetric method using [PERSON]'s reagent. Prior to analysis, cells were pelleted by centrifugation at 10,000\(\times\)\(g\) for 3 min. Then, 100 \(\mu\)L of the supernatant was diluted with deionized water, mixed on a vortex, and 100 \(\mu\)L of [PERSON]'s reagent was added. After 5 min of incubation, the absorbance was measured using a Lambda 35 spectrophotometer at 425 nm. The NH\({}_{4}\)\({}^{+}\)-N content was expressed in mg L\({}^{-1}\).  
The concentration of phosphate in the growth media was evaluated at the end of the cultivation using ion chromatography (Dionex ICS-900 Ion Chromatography System, Thermo Fisher Scientific, Wilmington, DE, USA) [16].