## 3 Results and Discussions
### Characteristics of the Development of Caliphora vicina on Different Substrates  
The _Calliphora vicina_ species studied prefer protein substrates for development because they cannot develop on every type of substrate. As could be noted during the study, the development in the species _Calliphora vicina_ occurs through complete metamorphosis, with stages clearly determined by morphological and structural characteristics.  
The analysis of the material observed has shown that the insect used for colonization preferred laying eggs on the softer and more humid surfaces of the organs [40; 41; 42; 43]. The larvae developed by executing perforations in substratum while feeding. In the case of samples from the tongue substratum, development in early stages took place in the middle part of the sample in the cross-section, after which the larvae migrated towards the surface of the sample. In the case of samples from the eye, larvae in all developmental stages used the interior of the eye to develop, not its exterior (Figures 2 and 3).  
Figure 2: Developmental stages, _Calliphora vicina_, pig tongue: (**a**)—eggs; (**b**)—larva stage I; (**c**)—larva stage II; (**d**)—larva stage III; (**e**)—pupa; (**f**)—juvenile.  
The acceleration of development in larvae stages depending on substratum is characterized by differences in terms of time, in favor of the tongue substratum during the first three stages, except the pupa stage, the difference being: stage I, 24 h; stage II, 48 h; and stage III, 24 h (Tables 1 and 2). Using these data and comparing them to the literature, we can conclude that the pig tongue substratum is more favorable for the development of _Calliphora vicina_ because it ensures a more nutritious content. Statistical differences of the average sizes on each substrate in each stage of the development cycle in _Calliphora vicina_ are shown in Figure 4.  
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multirow{2}{*}{**Developmental Stage**} & \multirow{2}{*}{**Temperature**} & \multirow{2}{*}{**Time (Hours)**} & \multirow{2}{*}{**Dimensions**} & \multirow{2}{*}{**Standard**} & \multicolumn{2}{c}{**95\% Confidence Interval between**} \\  & & & & **Average (mm)** & & **Limit** & **Limit** \\ \cline{5-6}  & & & & **Average (mm)** & & **Deviation** & **Inferior Limit** & **Superior Limit** \\  & & & & & **(mm)** & **(mm)** \\ \hline Eggs & 25 & - & - & - & & \\ Larva stage I & 25 & 48 & 3.29 & 0.75 & 2.59 & 3.98 \\ Larva stage II & 25 & 96 & 8.43 & 0.78 & 7.70 & 9.16 \\ Larva stage III & 25 & 168 & 13.85 & 1.06 & 12.86 & 14.84 \\ Pupa & 25 & 216 & 8.85 & 0.69 & 8.21 & 9.49 \\ Fly & 25 & 240 & Different & - & & \\ \hline \hline \end{tabular}
\end{table}
Table 1: Developmental stages, _Calliphora vicina_ (temperature, time, and body length), pig tongue, \(p\leq\) 0.05, survival rates 87.5 %.  
Figure 3: Developmental stages, _Calliphora vicina_, pig eye: (**a**)—eggs; (**b**)—larva stage I; (**c**)—larva stage II; (**d**)—larva stage III; (**e**)—pupa; (**f**)—juvenile.  
The first larval stage in the nutritionally richer substrate, i.e., on the pig's tongue, begins after approximately 48 h, while in the case of the pig's eyes, it starts slower, after 72 h. It should be noted that the actual development time compared to the time when the larval measurements were made varied by +/ 2-3 h.  
From the analysis of the variability in the body length of _Calliphora vicina_ in metamorphoses, where the variables are the development duration and the substratum type, we can notice that the larvae stage II and III on pig tongue have much greater dimensions compared to the same stages of development on pig eye. The variability in the body length of the ten individuals measured for each stage is given by the different structural components of the studied organs and the different approaches used by the larvae in the feeding process.