## 3 Discussion  
The effects of extraction solvents and the type of _Helichrysum_ species on the yield, chemical composition, and their activities were extensivity investigated using mainly the EO extracted from the plants [7; 8]. The current study findings clearly showed that the SS was only influenced by the type of extraction solvent but not by the type of _Helichrysum species_. This could be allied with the nature of the extraction solvent and chemical composition of individual species. Several studies have reported the effect of solvents on the SS of plant materials. The results of this study agree with the reports by [PERSON] et al. [1] who showed that acetone as an extraction solvent resulted in higher SS compared to ethanol. The relatively higher SS yield obtained by aqueous acetone (70%) could be due to the polarity of the solvents, as highly polar solvents are known to result in higher extract yield compared to less polar solvents. [PERSON] et al. [19] previously investigated the effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (_Phaselous vulgaris_) seeds. The authors also reported high extract yield obtained from polar solvents extracts and lower phenolic content compared to non-polar ones. The polarity of the solvents and the availability of the extractable plant constituents are the major contributing factors to variations in extraction yields. As the polarities of acetone and ethanol are 0.36 and 0.65, respectively [20], they were affected by the amount of water in the reaction matrix used in the current study. This further confirms that an extraction solvent with higher polarity is more effective for maximal extractable yield. According to [PERSON] et al. [21], the addition of water to organic solvents increases the polarity of the solvents, hence the higher SS contents observed in the 70% acetone-based extracts than in 95% ethanol extracts used in the current study. [PERSON] et al. [1] further elucidated a significant correlation between high polar solvents and high extract yield. The authors reported a 40% reduction in the soluble solid yield of 95% ethanol than those of 70% acetone-based extracts. Therefore, based on the current study and the other studies stated above, aqueous acetone was the better extraction solvent to obtain a higher SS from _Helichrysum_ species. In this study, different extraction solvents showed a significant effect on the extraction yield of total polyphenol (TP) [22]. There are a few examples of literature about the TP  
Figure 1: **(A)** Antifungal screening of aqueous acetone and ethanol extracts of _H. odoratisimum_ (L.) and _H. pathum_ by disk diffusion method against _Botrytis cinerea_. Mean values (\(n=9\)) of microbial percentage inhibition with standard error bars. Different letters are significantly different based on [PERSON]’s Multiple Range Test at \(p\leq 0.05\). (**B**) Pareto analysis showing the effects of extraction solvent, variation in Spp., and interaction effects at 95% confidence indicated as the vertical red line. (**C**) Statistical parameters. * Colour only online. content of _Helichrysum_ species stem and leaf extracts. [PERSON] et al. [1] reported a 25% difference in the TP content of extracts obtained with 70% acetone and 95% ethanol of stems and leaves of _H. odoratissimum_. This discrepancy could be related to the polarity of the solvents used, as it was reported by [PERSON] et al. [23] that the extractable phenolic compounds depend on the type of solvent used, its polarity index, and the solubility of phenolic compounds in the extraction solvent. Another study by [PERSON] et al. [24] reported a reduction in active compounds obtained using 90% ethanol for pomerangate (peel, leaf, mesocarp, and seed) extracts compared to the 50% and 70% ethanol filtrates. The reduction in TP was associated with the highest solvent concentration of ethanol. Generally, high ethanol concentration leads to protein denaturation, preventing the dissolution of polyphenols leading to lowered extraction rate [25].