## 3 Results and Discussion
### Effect of Genotype on Total and Individual Anthocyanins
\begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline  & \multicolumn{10}{c}{**Culivar**} \\ \hline
**location** & **Year** & **Can** & **Cle** & **Flo** & **Fri** & **Gar** & **Son** &
\begin{tabular}{c} _Yearly_ \\ _Mean per_ \\ _Location_ \\ \end{tabular} \\ \hline
**Norway** & **2017** & 0.4 \(\pm\) 0.6 & 46.7 \(\pm\) 7.7 & 32.6 \(\pm\) 5.9 & 80.9 \(\pm\) 10.3 & 78.7 \(\pm\) 19.1 & 32.0 \(\pm\) 5.3 & _45.2 A_ \\
**2018** & 0.2 \(\pm\) 0.1 & 37.3 \(\pm\) 8.6 & 41.3 \(\pm\) 7.9 & 72.2 \(\pm\) 24.5 & 58.0 \(\pm\) 9.3 & 24.2 \(\pm\) 5.5 & _38.9 A_ \\  & _mean_ & _0.3 a_ & 42.0 b_ & 36.9 b & 76.5 c & _68.3 c_ & _28.1 b_ & \\ \hline
**Poland** & **2017** & 0.2 \(\pm\) 0.4 & 52.9 \(\pm\) 10.4 & 36.6 \(\pm\) 8.0 & 87.8 \(\pm\) 17.9 & 69.2 \(\pm\) 11.8 & 42.4 \(\pm\) 7.5 & _48.2 A_ \\  & _2018_ & 2.8 \(\pm\) 0.3 & 49.4 \(\pm\) 3 & 52.5 \(\pm\) 9.9 & 82.4 \(\pm\) 16.1 & 68.1 \(\pm\) 12.7 & 48.4 \(\pm\) 8.2 & _50.6 A_ \\  & _mean_ & _1.5 a_ & _51.2 b_ & 44.5 b_ & _85.1 d_ & _68.6 c_ & _45.4 b_ & \\ \hline
**Germany** & **2017** & 0.4 \(\pm\) 0.3 & 59.2 \(\pm\) 12.8 & 33.9 \(\pm\) 16.7 & 94.8 \(\pm\) 10.5 & 71.1 \(\pm\) 18.8 & 46.7 \(\pm\) 14.4 & _51.0 A_ \\  & **2018** & 0.0 \(\pm\) 0.0 & 59.2 \(\pm\) 6.4 & 51.1 \(\pm\) 8.5 & 75.9 \(\pm\) 12.8 & 81.6 \(\pm\) 10.6 & 44.9 \(\pm\) 11.8 & _52.1 A_ \\  & _mean_ & _0.2 a_ & 59.2 c_ & 42.5 b & _85.4 d_ & _76.4 d_ & _45.8 b_ & \\ \hline
**Italy** & **2017** & - & 64.1 \(\pm\) 11.0 & - & 87.8 \(\pm\) 13.0 & 98.8 \(\pm\) 7.7 & 40.0 \(\pm\) 5.9 & _72.6 B_ \\  & **2018** & - & 66.3 \(\pm\) 13 & - & 71.5 \(\pm\) 13.3 & 61.3 \(\pm\) 8.8 & 27.3 \(\pm\) 7.4 & _56.6 A_ \\  & _mean_ & - & _65.2 b_ & - & _79.6 b_ & _80.1 b_ & _33.6 a_ & \\ \hline
\begin{tabular}{c} \(X\) \\ _year_ \\ _’_’_ \\ \end{tabular} \\ \hline \hline \end{tabular}
\end{table} 
Table 7: Effect of growing location and year on pelargonidin-3-(6-O-malonyl)-glucoside content as the mean of two picking dates in fruits of six strawberry cultivars. The genotype influenced significantly (\(p\leq 0.05\)) the abundance of total and individual anthocyanins in the fruits (Tables 2-7). When considering the cultivar means for all locations, 'Florence' and 'Frida' showed the highest total anthocyanin content (426.1 and 408.0 mg kg\({}^{-1}\) fresh weight (FW), respectively) whereas 'Sonata' had the lowest (193.2 mg kg\({}^{-1}\) FW) (Table 2). As expected, total anthocyanin content was strongly related to the predominant anthocyanin pel-3-glc (Table 4), resulting in the highest content for 'Florence' (357.4 mg kg\({}^{-1}\) FW) and, however, being significantly different from 'Florence', for 'Frida' (286.3 mg kg\({}^{-1}\) FW) and the lowest for 'Sonata' (139.3 mg kg\({}^{-1}\) FW) and also for 'Gariguette' (156.4 mg kg\({}^{-1}\) FW). Thereby, pel-3-glc contribution to the total anthocyanin content in the cultivars was in the range of 66-84%. 'Florence' (20.5 mg kg\({}^{-1}\) FW) also had, on average, a higher content of cya-3-glc compared to the other cultivars (Table 3). Pel-3-rut was highest in 'Candonga' and 'Frida' (21.8 and 21.3 mg kg\({}^{-1}\) FW) (Table 5), whereas cya-3-mal-glc was enriched in 'Florence' and 'Frida' (20.5 and 13.5 mg kg\({}^{-1}\) FW) (Table 6). The level of pel-3-mal-glc, the second abundant anthocyanin in strawberry, was again highest in 'Frida' (83.3 mg kg\({}^{-1}\) FW) and in 'Gariguette' (75.8 mg kg\({}^{-1}\) FW) (Table 7). The contribution of pel-3-mal-glc varied widely in a range of 9.9-31.7% of the total anthocyanin. In contrast to the other investigated cultivars, 'Candonga' contained only cya-3-glc, pel-3-glc, and pel-3-rut in large quantities, while cya-3-mal-glc and pel-3-mal-glc were only found in very small amounts in some locations and years (on average <0.4 and 0.7 mg kg\({}^{-1}\) FW). Each cultivar had an individual anthocyanin profile that will be discussed later (see Section 3.3). In general, the values for total and individual anthocyanin are in similar ranges as previously reported for these cultivars [11; 12; 14; 21; 30; 31; 32].