## 3 Results and Discussion
### Chemical Composition of EOs  
2.1 Effects of the Distillation Method on the Chemical Composition of the Investigated Leaf EO Samples (EO-HD and EO-WSD)  
Figure 1A,B show the total ion current (TIC) chromatogram recorded by GC-MS of the studied leaf EO samples, and their chemical compositions are shown in Table 3 and Figure 2.  
Figure 1: Total ion current (TIC) chromatograms on a DB-1 column of the essential oil from Azorean _Cryptomeria japonica_ leaves, isolated using the hydrodistillation (**A**) and water-steam distillation (**B**) methods. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Components**} & \multirow{2}{*}{**RI**} & \multicolumn{4}{c}{**Relative Content (\%)**} & \multirow{2}{*}{**IP 3**} \\ \cline{3-3} \cline{5-6}  & & **OE–HD 1** & & **OE–WSD 1** & & **OE–SD 2** \\ \hline Monoterpene Hydrocarbons & & & & & \\ (MH) & & & & & \\ Tricyclene & 921 & 0.5 & 0.6 & 0.6 & c, d \\ \(\alpha\)-Thujene & 924 & 2.1 & **2.8** & 1.6 & c, d \\ \(\alpha\)-Pinene & 930 & **34.5** & **46.4** & **39.3** & a, c, d \\ Camphene & 938 & **3.5** & **3.8** & **3.7** & c, d, e \\ Sabinene & 958 & **20.2** & **11.6** & **19.3** & a, c, d, e \\ \(\beta\)-Pinene & 963 & t & 1.7 & 2.1 & a, c, d, e \\ \(\beta\)-Myrcene & 975 & **5.9** & **5.8** & **5.7** & a, c, d, e \\ \(\alpha\)-Phellandrene & 995 & t & 0.1 & 0.1 & c, d, e \\ \(\delta\)-3-Carene & 1000 & 1.1 & 1.1 & 2.6 & a, e \\ \(\alpha\)-Terpinene & 1002 & 1.0 & 1.1 & 1.2 & a, c, d, e \\ \(p\)-Cymene & 1003 & 0.7 & **4.4** & 0.3 & a, c, d, e \\ \(\beta\)-Phellandrene & 1005 & 0.4 & 0.7 & 0.8 & c, e \\ Limone & 1009 & 1.4 & **3.2** & **8.3** & a, c, d, e \\ \(\gamma\)-Terpinene & 1035 & 1.1 & **2.6** & 1.9 & a, c, d, e \\ Terpinolene & 1064 & 0.4 & 0.8 & 1.0 & a, c, e \\ \hline Oxygenated Monoterpenes & & & & & \\ (OCM) & & & & & \\ _trans-2-p_-Menthen-1-ol & 1099 & - & - & 0.1 & e \\ Bornel & 1134 & - & - & 0.1 & a, c \\ Terpinen-4-ol & 1148 & 1.8 & **5.4** & 2.0 & a, c, d, e \\ \(\alpha\)-Terpinol & 1186 & - & 0.2 & - & a, c, d, e \\ Linaly acetate & 1245 & - & - & 0.1 & b, c \\ Bornyl acetate & 1265 & 1.3 & 1.6 & 1.7 & b, d \\ \(\alpha\)-Terpenyl acetate & 1334 & - & - & 0.1 & b, c, d \\ \hline Sesquietpene Hydrocarbons & & & & & \\ (SH) & & & & & \\ \(\beta\)-Elemene & 1388 & - & - & 0.2 & c, d \\ \(\beta\)-Caryophyllene & 1414 & - & - & 0.2 & c, f \\ \(\gamma\)-Muurolene & 1469 & - & - & 0.7 & c \\ \(\alpha\)-Muurolene & 1494 & - & 0.2 & 0.3 & c \\ \(\gamma\)-Cadinene & 1500 & - & 0.2 & 0.4 & c, d \\ trans-Calamene & 1505 & - & - & t & c \\ \(\delta\)-Cadinene & 1505 & 1.6 & 1.2 & 1.1 & c, d \\ \hline Oxygenated Sesquietrpenes & & & & & \\ (OCS) & & & & & \\ \(\gamma\)-Eudesmol & 1609 & 1.2 & 0.2 & - & g \\ \(\tau\)-Cadinol & 1616 & 0.2 & 0.1 & 0.2 & c, d \\ \(\tau\)-Muurolol & 1616 & 0.3 & 0.1 & - & g \\ \(\beta\)-Eudesmol & 1620 & 1.6 & 0.2 & - & c \\ \(\alpha\)-Eudesmol & 1634 & **3.4** & 0.3 & - & c \\ \hline \multicolumn{6}{c}{Diterpene Hydrocarbons} & & & \\ (DH) & & & & \\ Sandaracopimara-8(14),15- & & & & & \\ diene & & & & & \\ Phylolcadene & 2006 & **2.9** & 0.2 & 0.9 & a \\ \hline \multicolumn{6}{c}{Identified components (\%)} & 97.6 & 98.1 & 97.4 \\ \hline \hline \end{tabular} 1
[FOOTNOTE:1]Footnote 1: Data from our study. 2 Data provided by [PERSON], [PERSON] 3 Identification procedure (IP): a = commercial standards (Sigma-Aldrich-Fluka); b = laboratory-synthesized components following the methods described by [PERSON] et al. [45]; c = reference EO (_Thymus casegititins_) [43]; d = reference EO (_Laurus azorica_) [44]; e = reference EO (_Coriandrum sativoun_, Saturja montana, Santolina chamacyparissus_, and _Thymus vulgaris_) [45]; f = reference EO (_Monizia eublis_) [46]; g = MS, RI, \({}^{13}\)C-NMR. RI-linear retention indices relative to C\({}_{9}\)–C\({}_{21}\) n-alkanes on a DB-1 column; t-trace (<0.05%).  
\end{table}