## 4 Discussion
In our experiment, we cannot exclude the effect of an incomplete dilution of the _M. pulcherrina_ inoculum in the SF condition (i.e., the presence of a more concentrated _M. pulcherrina_ in the sampling volume in the tank even after mixing), since only a short time (approximately 1 h) elapsed between the inoculum and the sampling, making the direct bioprotective effect of _M. pulcherrina_ unlikely. Thus, our results are interesting, but the bioprotective effect in the clarified must needs to be studied more thoroughly, with sampling for the determination of microbial communities at precise time points prior to _S. cerevisiae_ inoculum. However, the relative abundance does not provide functional information about the microorganisms analyzed (living or dead cells). Therefore, in future bioprotection experiments, it will be essential to complement molecular methods with functional analysis in flow cytometry to obtain the necessary physiological information. At the end of the alcoholic fermentation, stabilization, and filtration phases, the wines were ready for the tasting session by the Agroscope panel. Our results did not show any dramatic change in the Chasselas produced with _M. pulcherrina_, which is important for maintaining the overall sensory expectations of this specific wine. As with most grape varieties, the action of the yeast during alcoholic fermentation can modulate the aromatic profile of Chasselas wine. In this context, we found that the SF conditions imparted a more \"floral\" character to the wine, although the differences were not statistically significant. We also found a statistically significant difference in the \"lactic\" character, which was more pronounced in the SF condition. The floral character was described in varieties such as Airen by [PERSON] et al. (2017) after the ternary fermentation of \(S\). _cerevisiae_ with _M. pulcherrima_ and _L. thermotolerans_. To the best of our knowledge, the lactic character has not yet been described in winemaking with _M. pulcherrima_, and it would be interesting to carry out a molecular analysis of the aroma compounds to evaluate, along with the other descriptors, the presence of specific molecules. Finally, our results show that the panel's overall preference was skewed toward the CC and SF rather than the PDC. This is interesting because it suggests that the SF with the chosen _M. pulcherrima_ is efficient in creating a wine that is enjoyable and expresses new characteristics without completely changing its original character, resulting in a good alternative to PDC vinications. However, several aspects remain to be clarified, and additional data need to be collected, especially regarding the bioprotective effect in must after clarification and the _M. pulcherrima_/_S.cerevisiae_ ratio in cellar-scale assays. Overall, these data suggest that our bioprospecting strategy is effective in screening and selecting yeasts that can be used at the cellar scale to contribute to the creation of new products in a safe and efficient manner. This is important in a scenario where the use of indigenous microorganisms is becoming increasingly important to wine producers and consumers. The following supporting information can be downloaded at: [[https://www.mdpi.com/article/10.3390/foods12244485/s1](https://www.mdpi.com/article/10.3390/foods12244485/s1)]([https://www.mdpi.com/article/10.3390/foods12244485/s1](https://www.mdpi.com/article/10.3390/foods12244485/s1)), Figure S1: FCM Gating Strategy. Conceptualization, F.L. and B.B. ; methodology, F.S., M.B., F.L., H.B. and B.B. ; data curation, F.L., B.B. and H.B. ; validation, F.S., S.B. ([PERSON]) and B.B. ; formal analysis, F.S. ; investigation, F.S., C.D., Y.B., M.B., L.A., S.B. ([PERSON]), F.V., C.M., H.B., S.S., M.C., C.C. and B.B. ; resources, J.-L.S., H.B., S.B. ([PERSON]), F.L., C.C., G.B. and B.B. ; writing--original draft preparation, F.S. ; writing--review and editing, F.S., M.B., F.L., S.B. ([PERSON]), H.B., S.S., G.B. and B.B. ; visualization, F.S. ; supervision, G.B. and B.B.