# Elemental Profiles of Legumes and Seeds in View of Chemometric Approach
## 3 Results and Discussion
### Influence of the Product Type (Legumes, Seeds) and Its Botanical Family on Metal Concentrations  
A statistically significant result of the Kruskal-Wallis test means that our independent variable affects the dependent variable. In our study, the Kruskal-Wallis test allowed the verification of the influence of the product type (legumes, seeds) and its botanical family (Fabaceae, Papaveraceae, Cucurbitaceae, Asteraceae, Linaceae, Pedaliaceae) on metal concentrations. There were found differences in Mg, Na, K, P, Zn, Cu, Mn, Cr, and Cd content depending on the type of the analyzed product (Table 5). In the case of botanical provenance, such statistical dependence was recorded for most of the analyzed components, except for Fe, Cr, and Co (Table 5).  
\begin{table}
\begin{tabular}{c c c} \hline
**Element** & **Legumes** & **Oilseeds** \\ \hline Mg & (+)Ca \({}^{c}\), (+)K \({}^{c}\) & (+)P \({}^{c}\), (+)Fe \({}^{a}\), (\(-\))Cd \({}^{a}\) \\ \hline Ca & (+)Mg \({}^{c}\), (+)K \({}^{b}\), (+)Mn \({}^{a}\), (+)Cr \({}^{b}\), (+)Ni \({}^{b}\) & (+)Cr \({}^{b}\), (+)Cd \({}^{a}\) \\ \hline Na & - & (\(-\))Mn \({}^{a}\) \\ \hline K & (+)Mg \({}^{c}\), (+)Ca \({}^{b}\), (+)P \({}^{b}\) & - \\ \hline P & (+)K \({}^{b}\), (+)Zn \({}^{a}\), (+)Cd \({}^{b}\) & (+)Mg \({}^{c}\), (+)Zn \({}^{c}\), (+)Fe \({}^{b}\) \\ \hline Zn & (+)P \({}^{a}\), (+)Cd \({}^{b}\) & (+)P \({}^{c}\) \\ \hline Cu & (+)Fe \({}^{a}\), (+)Co \({}^{b}\), (+)Cr \({}^{a}\), (+)Ni \({}^{a}\) & - \\ \hline Fe & (+)Cu \({}^{a}\), (+)Cr \({}^{a}\), (+)Ni \({}^{a}\), (+)Co \({}^{a}\) & (+)Mg \({}^{a}\), (+)P \({}^{b}\) \\ \hline Mn & (+)Ca \({}^{a}\), (+)Ni \({}^{b}\) & (\(-\))Na \({}^{a}\) \\ \hline Cr & (+)Ca \({}^{b}\), (+)Cu \({}^{a}\), (+)Fe \({}^{a}\) & (+)Ca \({}^{b}\) \\ \hline Co & (+)Cu \({}^{b}\), (+)Ni \({}^{b}\), (+)Fe \({}^{a}\) & - \\ \hline Ni & (+)Ca \({}^{b}\), (+)Cu \({}^{a}\), (+)Fe \({}^{a}\), (+)Mn \({}^{b}\), (+)Co \({}^{b}\) & - \\ \hline Cd & (+)P \({}^{b}\), (+)Zn \({}^{b}\) & (+)Ca \({}^{a}\), (\(-\))Mg \({}^{a}\) \\ \hline \end{tabular} \({}^{a}\) \(p<0.05\); \({}^{b}\)\(p<0.01\); \({}^{c}\)\(p<0.001\).  
\end{table}
Table 4: Significant correlations between content of elements in legumes and oilseeds.  
\begin{table}
\begin{tabular}{c c c c c c c c c c c c c} \hline  & **Mg** & **Ca** & **Na** & **K** & **P** & **Zn** & **Cu** & **Fe** & **Mn** & **Cr** & **Co** & **Ni** & **Cd** \\ \hline Type & 18.8 \({}^{c}\) & 1.22 & 5.44 \({}^{a}\) & 13.8 \({}^{c}\) & 13.4 \({}^{c}\) & 17.5 \({}^{c}\) & 11.6 \({}^{c}\) & 1.99 & 14.6 \({}^{c}\) & 6.44 \({}^{a}\) & 0.01 & 0.90 & 13.8 \({}^{c}\) \\ \hline Botanical family & 21.2 \({}^{c}\) & 13.0 \({}^{a}\) & 13.2 \({}^{a}\) & 17.0 \({}^{b}\) & 17.8 \({}^{b}\) & 19.2 \({}^{b}\) & 16.6 \({}^{b}\) & 8.50 & 18.5 \({}^{b}\) & 11.1 & 5.49 & 12.0 \({}^{a}\) & 17.9 \({}^{b}\) \\ \hline \end{tabular} \({}^{a}\)  
\end{table}
Table 5: The results of the ANOVA Kruskal-Wallis test of legumes and oleic seeds’ elemental composition (results are expressed as H value).  
However, based on the result of the analysis of variance, we were not able to determine exactly which groups differ from each other in view of their botanical affinity. Therefore, a post-hoc analysis using multiple step-down comparisons was performed that enabled us to answer the question of which of the analyzed groups were different. Statistically significant differences (\(p<0.05\)) were noted for the following elements: Ca (Cucurbitaceae vs. Papaveraceae), Mg (Cucurbitaceae vs. Fabaceae), K (Pedaliaceae vs. Fabaceae; Asteraceae vs. Fabaceae), P (Cucurbitaceae vs. Fabaceae), Zn (Cucurbitaceae vs. Fabaceae), Cu (Asteraceae vs. Fabaceae), Ni (Asteraceae vs. Pedaliaceae), Mn (Papaveraceae vs. Fabaceae; Cucurbitaceae vs. Fabaceae), and Cd (Linaceae vs. Fabaceae).