## 2 Materials and Methods
### Measurement of Blood Pressure and Area under the Curve (AUC) Analysis  
Blood pressure at the mouse caudal artery was measured biweekly beginning at 5 weeks of age until 29 weeks of age. Measurements were consistently made between 0900 and 1200 h on the day of the experiment. The 24 mice were divided into two groups of 12 mice each for testing on two consecutive week days, specifically, Monday, group one of \(n=12\) mice; Tuesday, group two of \(n=12\) mice. Within each group of 12 mice, there were \(n=4\) mice from each of the three diet groups. The order of testing was as follows: Group 1: CON-1, 5 HS-1, 15 HS-1, etc., ending with CON-12, 5 HS-12, 15 HS-12; Group 2: CON-13, 5 HS-13, 15 HS-13, etc., ending with CON-24, 5 HS-24, 15 HS-24. This protocol ensured that each mouse was tested at the same time of day to minimize variation in their daily routine and that the three diet groups were evenly distributed over the 3 h test period. To minimize stress, precautions described in the protocols literature for mice [24; 25; 26] were strictly followed. This included maintaining a calm, quiet, dimly lit, and warm environment, with no extraneous odors and no perfumes when performing experiments. The mouse holding tube, tail cuffs, and work area were cleaned with 70% ethanol after each animal test. The same two researchers, with prior training and following the same protocol, performed blood pressure measurements. Each person was assigned one of the two groups of \(n\)=12 mice for the entire duration of the study. They were not blinded \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Ingredient, g per kg Diet** & **Control Diet a** & **5\% Hempseed** & **15\% Hempseed** \\ \hline Casein, High Nitrogen & 200 & 185 & 155 \\ L-Cystine & 3 & 3 & 3 \\ Sucrose b & 100 & 100 & 100 \\ Cornstarch & 397.486 & 395.99 & 392.997 \\ Dytrose & 132 & 132 & 132 \\ Soybean Oil & 70 & 52 & 16 \\ t-Butylhydroquinone & 0.014 & 0.01 & 0.003 \\ Cellulose & 50 & 34.5 & 3.5 \\ Mineral Mix \#210025 c  & 35 & 35 & 35 \\ Vitamin Mix \# 310025 d  & 10 & 10 & 10 \\ Choline Bitartrate & 2.5 & 2.5 & 2.5 \\ Hempseed e  & 0 & 50 & 150 \\ Total & 1000 & 1000 & 1000 \\ Kilocalories per kg & 3760 & 3814 & 3922 \\ \hline \hline \end{tabular}  
* AIN-93G. b Ninety percent tetrasaccharides and higher; c Composition (g/kg mineral mix): CaCO\({}_{3}\), 357.0; KH\({}_{2}\)PO\({}_{4}\), 196.0; K Citrate-H\({}_{2}\)O\({}_{7}\), 70.78; NaCl, 74.0; K\({}_{2}\)SO\({}_{4}\), 46.6; MgO, 24.3; Fe citrate, 6.06; ZnCO\({}_{3}\), 1.65; MnCO\({}_{3}\), 0.63; CuCO\({}_{3}\), 0.31; KIO\({}_{3}\), 0.01; Na\({}_{2}\)SeO\({}_{4}\), 0.01025; (NH\({}_{4}\)) 6 Mo\({}_{7}\)O\({}_{24}\) 4H\({}_{2}\)O, 0.00795; Na\({}_{2}\)SiO\({}_{9}\)H\({}_{2}\)O, 1.45; CrK(SO\({}_{4}\)) 2.124;O, 0.275; (L\({}_{1}\)O, 0.0174; H\({}_{3}\)O\({}_{8}\), 0.0815; Na\({}_{2}\), 0.0635; 2 NICO\({}_{3}\)-3 Ni(OH) 2-4H\({}_{2}\)O, 0.0381; NH\({}_{4}\)VO\({}_{3}\), 0.0066; 4 Composition (g/kg vitamin mix): thiamin thickness, 0.6; riboflavin, 0.6; pyridilocyne HICI, 0.7; nicoturic acid, 3.0; Ca pantothane, 1.6; folic acid, 0.2; D-biotin, 0.02; vitamin B\({}^{12}\) (0.1% in mannitol), 2.5; vitamin A palmitate (500,000 IU/g), 0.8; DL-\(\alpha\)-tocopherol acetate (500 IU/g), 15; vitamin D3 (400,000 IU/g), 0.25; vitamin K/destructive 10 mg/g (phylloquinone), 7.5.
* Whole, ground hempseed (nutrition information supplied in Supplementary Table S1).  
\end{table} 
Table 1: Composition of experimental diets. to the animal identification. Mice were weighed prior to blood pressure measurement collection as part of the weekly weighing and recording procedure. Blood pressure was measured using the CODA Noninvasive Blood Pressure System and accompanying CODA Software, v. 4.1 (Kent Scientific Corporation, Torrington, CT, USA) following the manufacturer's methods and protocol for mice as described previously in the literature [24; 25; 26].