## 4 Discussion
The ability of a drug to selectively inhibit cancer cells while being less cytotoxic to normal cells is a beneficial drug property in medical research; this property is especially useful in combatting drug resistance in cancer [73]. Regarding our results demonstrating the cytotoxic effect of MSP-RT towards MCF-7, we tested the cytotoxicity of MSP-RT towards MRC-5 and Vero cell lines. These two cell lines were derived from the fibroblast tissues of human fetal lungs [74] and from the monkey kidney epithelial cells, respectively [75]. They are regarded as normal cell lines [76]. This study showed that the MRC-5 and Vero cell lines were less responsive to MSP-RT than the MCF-7 cell lines, as IC\({}_{50}\) values of 2.6 folds higher than that seen in MCF-7 cells were observed in these normal cells. As a result of this, _Moringa_ seed crude polysaccharide (MSP-RT) extract may shield normal cells from the toxicity of chemotherapeutics while having no negative side effects on normal cells. It may also have antitumor and cancer-prevention properties [77; 78] This is an indication that this crude polysaccharide extract may be promising as a valuable source for novel cancer therapy. In the mass spectrum of MSP-RT crude polysaccharide extract peaks at \(m/z\) (ESI TOF MS ES-), 570.0203 can be observed (Supplementary Materials). The NMR and mass analyses confirm that the main product was 4-\(\alpha\)-rhamnopyranosyloxy-benzyl glucosinolate. Glucosinolates are compounds synthesized naturally in _Moringa__deifera_ and other members from _Brassicales_; these glucosinolates are converted into isothi cyanates when the plant tissues undergo some physical damage (trituration or mastication). These compounds from the plant tissues have been shown to have hypoglycemic, antioxidant, anti-inflammatory,and anticancer activities [79]. The mechanism of the cytotoxic activity of glucosinolates has been connected to their breakdown product isothiyanates (ITC) [80; 81]. The mechanism of cytotoxic action of ITC includes maintenance of low-systemic oxidative stress, cell-cycle progression inhibition, angiogenesis inhibition, and apoptotic induction in cancer cells [82]. The principal objective of the present study was the screening and evaluation of the antioxidative and antiproliferative properties of crude polysaccharide extracts obtained from selected plants. Natural compounds are being explored for medicinal purposes either to examine their potential as substitutions for toxic conventional chemotherapeutic agents or, better still, to assess their synergistic effect when used in combination with them. Through the elucidation of antioxidative and antiproliferative potentials of the crude polysaccharide extracts from the plants in this study, we contributed to the ongoing examination of plant-based ingredients as viable candidates for future therapeutic progress. The variations observed in % yield, total protein, total sugar, and phenolics content among crude polysaccharide extracts from different plants reflect the diverse biochemical compositions inherent in each plant species, and these variations may explain why they exhibit different bioactivities. The differences in composition may arise from a combination of many factors. The observed variations in the antioxidative activities of these crude plant polysaccharide extracts indicate that they responded differently to different free radical models. Of all the crude polysaccharides, significant antioxidative capacities were exhibited by crude polysaccharides from _Ocimum santum_ leaves, _Moringa oleifera_ leaves, and _Ginkgo biloba_ leaves, showing the highest significant activity in all the tested antioxidant models. It was also noted that the crude polysaccharide extracts with significantly higher phenolics than the others showed significant antioxidative activities, which is an indication that their antioxidative activities are associated with their phenolic component.