# _Forentation_
## 3 Results
### Fermentation Kinetics
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multicolumn{1}{c}{**Kinetic**} & \multicolumn{2}{c}{**Kinetic**} & \multicolumn{2}{c}{**Kinetic**} & \multicolumn{2}{c}{**Kinetic**} \\ \multicolumn{1}{c}{**Parameters**} & \multicolumn{2}{c}{**Parameters**} & & & \\ \hline \multicolumn{1}{c}{**P**} & \multicolumn{1}{c}{**Seq P-S**} & \multicolumn{1}{c}{**S**} & \multicolumn{1}{c}{**Sim I:1**} & \multicolumn{1}{c}{**Sim I:0**} & \multicolumn{1}{c}{**Seq S-P**} \\ \multicolumn{1}{c}{**’Sigmoid Model**} & \multicolumn{1}{c}{**\(\ast\)**Exponential Model**} & \multicolumn{1}{c}{**\(\ast\)**Exponential Model**} & \multicolumn{1}{c}{**\(\ast\)**Exponential Model**} & \multicolumn{1}{c}{**\(\ast\)**Exponential Model**} & \multicolumn{1}{c}{**\(\ast\)**Exponential Model**} \\ \multicolumn{1}{c}{A (\({}^{\circ}\)Brix)} & \(4.78\pm 0.30\) a & \(4.38\pm 0.04\) a & D (Brix) & \(2.80\pm 0.15\) a & \(2.94\pm 0.05\) a & \(2.82\pm 0.01\) a & \(2.77\pm 0.05\) a \\ C & \(8.46\pm 0.32\) b & \(9.53\pm 0.06\) a & S & \(10.20\pm 0.15\) a & \(10.17\pm 0.06\) a & \(10.34\pm 0.01\) a & \(10.19\pm 0.06\) a \\ K (\({}^{\circ}\)Brix, h - 1) & \(0.008\pm 0.0012\) b & \(0.012\pm 0.0001\) a & K (\({}^{\circ}\)Brix, h - 1) & \(0.0060\pm 3\times 10^{-4}\) b & \(0.0066\pm 9\times 10^{-5}\) a & \(0.0069\pm 6\times 10^{-5}\) a & \(0.0056\pm 2\times 10^{-4}\) b \\ M (h) & \(316.3\pm 33.4\) a & \(178.7\pm 1.0\) b & & & & \\ \hline \multicolumn{1}{c}{Different letters in the same row indicate differences between values obtained for the same model (ANOVA and Tukey test, n = 2). Fit of model in all cases with r\({}^{2}\geq 0.98\). P: _P. kudriuzzevii_ NPCC1651; S: _S. cerevisiae_ NIF8; Sim I:1: Simultaneous S:P ratio 1:1; Sim I:10: Simultaneous S:P ratio 1:10; Seq P-S: Sequential fermentation by S. _c. cerevisiae_ NIF8 48 h after _P. kudriuzzevii_ NPCC1651; Seq S-P: Sequential fermentation by _P. kudriuzzevii_ NPCC1651 48 h after _S. cerevisiae_ NIF8. \(\ast\) Sigmoid or altered Gompertz decay function, used previously by [PERSON] and [PERSON] [35]. A is the lower asymptote when t tends to infinity (t \(\rightarrow\infty\)), K is the kinetic constant (h \(-\) 1), C is the distance between the upper and lower asymptote, and M is the time when the inflection point is obtained. ** Exponential decay function, used previously by [PERSON] et al. [34]. D is a specific value when t \(\rightarrow\infty\), S is the estimated value of change, and K is the kinetic constant (h \(-\) 1).  
\end{table} 
Table 2: Kinetic parameters obtained from pure and mixed fermentations conducted with _P. kudriuzzevii_ _avzevii_ NPCC1651 and _S. cerevisiae_ NIF8. Figure 2: Glucose and fructose consumption in pure and mixed fermentations with _P. kudriuzzevii_ _NPCC1651 and _S. cerevisiae_ NIF8. Lines represent modeled values (m) of glucose (**A**) and fructose (**B**) over time for each fermentation trial, while points indicate observed values (o). P: _P. kudriuzzevii_ NPCC1651; S: _S. cerevisiae_ NIF8; Sim I:1: Simultaneous S:P ratio 1:10; Seq P-S: Sequential fermentation by _S. cerevisiae_ NIF8 48 h after _P. kudriuzzevii_ NPCC1651; Seq S-P: Sequential fermentation by _P. kudriuzzevii_ NPCC1651 48 h after _S. cerevisiae_ NIF8. As was observed for \({}^{\circ}\)Brix, glucose consumption kinetics in both pure fermentations inoculated with _P. Rudriavzevii_ (P) and sequential fermentation initiated with this strain (Seq P-S) fitted to the sigmoid decay model. Among them, the sequential fermentations exhibited the highest glucose consumption rate (parameter _K_) (Table 3). Conversely, for all other fermentations (S, Sim 1:1, Sim 1:10, and Seq S-P), glucose consumption kinetics fitted to an exponential decay model. Among them, the Sim 1:10 trial exhibited the lowest glucose consumption rate, with no significant differences detected among the other fermentations (Table 3). Regarding fructose consumption, all fermentations fitted the sigmoid decay model (Table 3), allowing the comparison of kinetic parameters among them.