## 3 Results
### Hempeseed Screenings Bioprocessing
#### 3.2.1 Hempseed Screenings Bioprocessing Treatments Increased the Solubility of Dietary Fibre (NSP)
\begin{table}
\begin{tabular}{l c c c c c c} \hline \hline \multirow{2}{*}{**Plant Metabolites** **(mg/Kg)**} & \multicolumn{2}{c}{**Raw**} & \multicolumn{2}{c}{**EM\_1**} & \multicolumn{2}{c}{**EC\_2**} \\ \cline{2-7}  & **Total Content** & **Content after** & **Total Content** & **Content after** & **Total Content** & **Content after** \\  & **(Initial)** & **In Vitro** & **(Initial)** & **In Vitro** & **(Initial)** & **(Initial)** & **Vitro Digestion** \\ \hline \(p\)-cumaric acid & 53.71 \(\pm\) 10.72 & 3.26 \(\pm\) 0.44 & 43.56 \(\pm\) 2.39 & 1.23 \(\pm\) 0.11 \({}^{*}\) & 89.09 \(\pm\) 4.22 & 1.53 \(\pm\) 0.05 \({}^{*}\) \\ protocatechurablehyde & 44.24 \(\pm\) 5.40 & 1.58 \(\pm\) 0.05 & 28.81 \(\pm\) 1.90 & 1.05 \(\pm\) 0.09 \({}^{*}\) & 37.38 \(\pm\) 1.06 & 1.52 \(\pm\) 0.12 \\ protocetubeic acid & 43.66 \(\pm\) 7.13 & 6.52 \(\pm\) 0.20 & 32.09 \(\pm\) 1.75 & 2.95 \(\pm\) 0.02 \({}^{*}\) & 49.01 \(\pm\) 1.85 & 5.47 \(\pm\) 0.44 \\ salicylic acid & 25.59 \(\pm\) 4.11 & 36.32 \(\pm\) 1.48 & 34.24 \(\pm\) 4.65 & 45.30 \(\pm\) 3.25 \({}^{*}\) & 40.04 \(\pm\) 1.11 & 48.77 \(\pm\) 1.55 \({}^{*}\) \\ \(p\)-hydroxybenzoic acid & 23.12 \(\pm\) 2.71 & 20.92 \(\pm\) 0.93 & 26.73 \(\pm\) 2.01 & 28.29 \(\pm\) 1.49 \({}^{*}\) & 32.00 \(\pm\) 2.01 & 31.35 \(\pm\) 0.58 \({}^{*}\) \\ vanillin & 16.67 \(\pm\) 0.65 & 1.61 \(\pm\) 0.21 & 16.34 \(\pm\) 0.73 & 2.05 \(\pm\) 0.37 & 25.77 \(\pm\) 2.58 & 19.8 \(\pm\) 0.22 \\ ferulic acid & 16.30 \(\pm\) 0.47 & 13.91 \(\pm\) 0.57 & 26.51 \(\pm\) 3.45 & 21.06 \(\pm\) 1.02 \({}^{*}\) & 47.59 \(\pm\) 2.27 & 36.97 \(\pm\) 1.03 \({}^{*}\) \\ vanillin acid & 14.97 \(\pm\) 0.93 & 4.48 \(\pm\) 0.36 & 19.91 \(\pm\) 1.33 & 10.35 \(\pm\) 0.33 \({}^{*}\) & 21.67 \(\pm\) 0.61 & 10.66 \(\pm\) 0.54 \({}^{*}\) \\ p-hydroxybenzaldehyde & 14.22 \(\pm\) 1.64 & 8.05 \(\pm\) 0.22 & 14.77 \(\pm\) 0.68 & 8.24 \(\pm\) 0.52 & 16.58 \(\pm\) 1.84 & 8.65 \(\pm\) 0.22 \\ syringaldehyde & 10.81 \(\pm\) 0.56 & 1.86 \(\pm\) 0.07 & 10.27 \(\pm\) 0.51 & 1.94 \(\pm\) 0.21 & 19.65 \(\pm\) 2.58 & 1.99 \(\pm\) 0.04 \\ benzoic acid & 5.77 \(\pm\) 0.27 & 7.04 \(\pm\) 0.71 & 7.26 \(\pm\) 0.60 & 7.74 \(\pm\) 1.06 & 6.11 \(\pm\) 0.85 & 7.36 \(\pm\) 1.14 \\ \(3\), 5-dimethoxy-4 & & & & & & \\ hydroxybenzoic acid & 5.22 \(\pm\) 0.49 & 0.92 \(\pm\) 0.09 & 5.55 \(\pm\) 0.75 & 1.32 \(\pm\) 0.05 \({}^{*}\) & 7.91 \(\pm\) 1.34 & 1.49 \(\pm\) 0.02 \({}^{*}\) \\ acid & & & & & & \\ phloretic acid & 3.14 \(\pm\) 0.32 & 5.41 \(\pm\) 0.27 & n.d. & 4.51 \(\pm\) 0.18 \({}^{*}\) & 10.63 \(\pm\) 0.45 & 4.52 \(\pm\) 0.31 \({}^{*}\) \\ caffe acid & 2.03 \(\pm\) 0.49 & 3.48 \(\pm\) 0.13 & 3.02 \(\pm\) 0.38 & 2.07 \(\pm\) 0.04 \({}^{*}\) & 7.93 \(\pm\) 1.32 & 3.01 \(\pm\) 0.07 \({}^{*}\) \\ genistic acid & n.d. & 7.15 \(\pm\) 0.44 & n.d. & 4.41 \(\pm\) 0.34 \({}^{*}\) & 30.41 \(\pm\) 2.62 & 26.45 \(\pm\) 1.15 \({}^{*}\) \\ homoprotocatechuric acid & n.d. & 4.61 \(\pm\) 0.54 & n.d. & 8.38 \(\pm\) 0.48 \({}^{*}\) & 20.28 \(\pm\) 1.07 & 8.54 \(\pm\) 0.67 \({}^{*}\) \\ \hline \hline \end{tabular}  
* n.d. means not detected (i.e., below the detection level). The total content (initial) represents the sum of the free, alkaline, and acid forms of individual metabolites measured by LC-MS/MS (Table SI). A \(t\)-test with FDR correction was used for group comparison between Raw and EM_1 as well as Raw and EC_2 (\(\uparrow\)) \(p<\) 0.01.  
\end{table} 
Table 3: The composition of metabolites (measured by targeted LC-MS/MS) measured in the fraction resulted after in vitro digestion of the raw and bioprocessed (EM_1 and EC_2). Values were expressed with mean of three replicates and standard deviation.