## 3 Results
### Stability and Suriviviviv of LPa 12/1 Strain in Eve-Goad Milk Yofibruts  
The count of the LPa 12/1 strain in its freeze-dried form reached 6.6 \(\times\) 10\({}^{7}\) cfu/g, i.e., 7. 82 cfu/g in log 10. After application, the strain LPa 12/1 showed sufficient stability and survival in the ewe-goat milk yoghurt. Its count reached 5.1 cfu/g (log 10) (Table 5) after 24 h. On day 7 (one week), the LPa 12/1 strain count was almost the same (5.15 cfu/g (log 10). However, on day 10, its increase of almost about one log cycle (0.95 log cycle) was noted (6.1 cfu/g, log 10). It was also stable on day 14. The LPA 12/1 strain contributed to the total LAB count on days 7, 10, and 14. On those days, the LAB count was higher and stable. LAB counts were also higher in E yoghurts than their counts in control (C-yoghurts). A difference of 0.46 log cycle was noted when checking after 24 h compared with LAB counts in E and C yoghurts. On day 7, a difference of 1.56 log cycles was found; then, the difference of 1.15 and 1.0 log cycles was noted when comparing E and C yoghurts, respectively. The commercial culture count was seemingly not influenced by the LPa 12/1 strain addition from the start of the experiment. On day 10, the LPa12/1 count was increased by almost 1 log cycle (Table 5). On day 14, the LPa 12/1 strain count decreased to the initial level. As mentioned, the initial pH of yoghurt reached the value of 3.94. It was almost the same in both E and C yoghurts after 24 h (Table 5). On day 7, the pH was increased in both experimental and control yoghurts; in E yoghurt, the pH reached 4.55. The pH was 4.89 in control-C yoghurt. On day 10, the pH value was stable in C yoghurt. However, in E yoghurt, a decrease in pH was noted. Finally, on day 14, in both yoghurts, almost the same pH was measured (4.0 and 4.05). It was not negatively influenced by the LPa 12/1 strain addition.  
\begin{table}
\begin{tabular}{c c c} \hline \(n=6\) & **PA** & **IPA** \\ \hline Sampling 30 EG & 61.5 \(\pm\) 1.05 \({}^{\text{a}}\) & 2.53 \(\pm\) 0.21 \\ \hline Sampling 30 CG & 60.0 \(\pm\) 1.30 & 2.82 \(\pm\) 0.11 \\ \hline Sampling 45 EG & 62.0 \(\pm\)2.37 \({}^{\text{b}}\) & 2.93 \(\pm\) 0.10 \\ \hline Sampling 45 CG & 61.0 \(\pm\) 0.84 & 2.82 \(\pm\) 0.11 \\ \hline \end{tabular} Sampling 0/1, before experiment; Sampling 30 EG and 30 CG, sampling on day 30 in the experimental and control groups; Sampling 45 EG and 45 CG, sampling on day 45 in the experimental and control groups; experiment, day 30—application of LPa 12/1, day 45, 15 days of LPa 12/1. Significantly higher PA was noted in EG on day 30 compared with day 45 (\({}^{\text{ab}}\)\(p<\) 0.001).  
\end{table}
Table 4: Phagocytic activity (PA) and index of PA (IPA) after LPa 12/1 strain application and cessation in blood of Balb/c mice in percentage (%).  
\begin{table}
\begin{tabular}{c c c c c} \hline  & **pH** & **LPa12/1** & **LAB** & **Amyl. Str.** \\ \hline E/24 h & 3.90 \(\pm\) 0.0 & 5.1 \(\pm\) 0.0 & 5.1 \(\pm\) 0.0 & 5.1 \(\pm\) 0.0 \\ \hline C/24 h & 3.91 \(\pm\) 0.0 & nt & 4.61 \(\pm\) 0.2 & 4.41 \(\pm\) 0.1 \\ \hline E/Day 7 & 4.55 \(\pm\) 0.2 & 5.15 \(\pm\) 0.1 & 6.1 \(\pm\) 0.2 & 5.1 \(\pm\) 0.0 \\ \hline C/Day 7 & 4.89 \(\pm\) 0.1 & nt & 4.54 \(\pm\) 0.5 & 5.30 \(\pm\) 0.1 \\ \hline E/Day 10 & 3.70 \(\pm\) 0.1 & 6.1 \(\pm\) 0.1 & 6.1 \(\pm\) 0.3 & 6.1 \(\pm\) 0.0 \\ \hline C/Day 10 & 4.85 \(\pm\) 0.2 & nt & 4.95 \(\pm\) 0.1 & 6.1 \(\pm\) 0.0 \\ \hline \end{tabular}
\end{table}
Table 5: Stability and survival of LPa 12/1 strain in ewe–goat milk yoghurts (expressed in cfu per g (cfu/g, log 10) \(\pm\) SD.