# Biological Control of Phototrophic Biofilms in a Show Cave: The Case of Nerja Cave
## 3 Results
The genus _Acanthamoeba_, uncultured members of the _Codonosigidae_ family and the genus _Sorodipolphys_ (_Stramenopiles_) in sample 2A were also noticeable. Cultivable genera such as _Balamuttia_ and _Chlamydomyxa_ were also identified. To summarize, within _Bacteria_, significant differences were found between the two biofilm samples collected from speleothems displaying abundant growth of phototrophic microorganisms. Considering taxonomic groups in proportions greater than 1%, the analysis reflected a great diversity in the taxonomic composition of each sample, with a total of 27 orders (Figure 4). Comparatively, the samples presented similar orders, and shared common groups. Thus, samples 1 and 2 shared 22 orders. The greatest differences between each sample were in the percentage of abundance of certain groups, among which an uncultured order of _Alphaproteobacteria_, _Rhizobiales_, _Oceanospirllales_, _Chromatiles_, _Rhodothermales_, _Dadabacteriales_, _Bacillus_ and the phototrophic _Spirulinales_ were remarkable in sample 1, while the abundances of _Rhodospirllales_, _Caulobacterales_, _Lysobacterales_, _Thermonicrobiales_, _Pseudonocardiales_, _Cytophagales_, _Bacillus_ and uncultured members of the phototrophic _Cyanophyceae_ were noticeable in sample 2. The _Eukaryota_ showed a total of 19 groups, of which 7 were found in sample 1, and 16 in sample 2. Both sampling sites solely shared 5 groups of eukaryotes: _Schizophreniadiida_, uncultured _Amoebozoa_ \begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & & & \multicolumn{3}{c}{**Abundance of Sequences (\%) \({}^{*}\)**} \\ \cline{3-6}  & & **1A** & **1B** & **2A** & **2B** \\ \hline \multirow{6}{*}{_Amoebozoa_} & _Dactylopodida_ & 17.7 & 2.6 & 0.0 & 0.0 \\  & _Discosea_ & _Acanthamoeba_ & 0.0 & 0.0 & 1.2 & 6.0 \\  & _Balamutthia_ & 0.0 & 0.0 & 0.4 & 1.1 \\  & _Schizophreniadiida_ & uncultured & 0.0 & 4.7 & 46.2 & 40.8 \\  & _Tubulina_ & _Echinamoeba_ & 10.5 & 0.4 & 0.0 & 0.0 \\  & uncultured & 6.8 & 8.3 & 0.0 & 0.0 \\  & Others & uncultured & 0.0 & 0.0 & 1.2 & 0.0 \\ \hline \multirow{6}{*}{_Opishtokotta_} & _Holozoa_ & _Codonosigidae_ & 0.0 & 0.0 & 0.5 & 8.5 \\  & _Rhabditida_ & 0.0 & 0.0 & 12.4 & 19.1 \\  & _Fungi_ & _Stachybutyrateaceae_ & 0.0 & 0.0 & 0.0 & 3.2 \\  & & uncultured & 0.2 & 0.2 & 0.2 & 1.4 \\ \hline \multirow{6}{*}{_SAR_} & _Alevolata_ & _Gregarina_ & 6.4 & 3.8 & 0.5 & 1.3 \\  & _Alveolata_ & uncultured & 0.0 & 0.0 & 1.7 & 0.0 \\  & _Colpodea_ & 0.0 & 0.0 & 4.2 & 3.3 \\  & _Rhizaria_ & _Heteromita_ & 0.0 & 0.0 & 12.2 & 8.2 \\  & & P34,6 & 0.0 & 0.0 & 0.0 & 2.9 \\  & _Stramenopiles_ & _Sorodipolphys_ & 0.4 & 0.7 & 5.8 & 1.6 \\  & & _Chlamydomyxa_ & 0.0 & 0.0 & 4.6 & 0.0 \\ \hline
**Unknown** & Others & Others & Others & 58.0 & 79.3 & 8.9 & 2.6 \\ \hline \hline \end{tabular} \({}^{*}\) Relative abundance \(>\)1% in at least one of the samples.  
\end{table} 
Table 2: Relative abundance of eukaryotes, with higher taxonomic level in bold and lower taxonomic levels in italics. uncultured _Fungi_, and the genera _Gregarina_ and _Sorodiplophrys_. In addition, the high abundance of unassigned sequences in sample 1 indicated the existence of large unknown taxa in this community.