## 3 Results  
### Selection of RF Current Parameters for WP Activation  
Depending on the intensity of LTP exposure, secondary effects develop in tissues, which are a complex of adaptive and compensatory reactions responsible for the activation of reparative processes [31, 34, 35, 36]. Based on this concept of activation, the first step was to optimize the frequency response of the high frequency (HF) current generator that initiates the LTP discharges.  
The choice of HF current parameters for WP activation was performed using an HF generator initiating cold plasma discharges at frequencies of 0.11, 2.64, and 6.78 MHz in the electrolyte matrix of biological tissue. A physiological solution (0.9% NaCl) with the addition of edible gelatin dissolved in water was used as a macroscopic analog of the electrolyte matrix of a biological tissue. The amount of dissolved gelatin was 1% of the volume of saline filling the cuvette. The study of the nature of LTP was carried out using a laboratory stand, as shown in Figure 2.  
Oscillograms of the curves of current (red curve) and voltage (yellow curve) for different LTP generation frequencies are shown in Figure 3.  
An analysis of the oscillograms in Figure 3 shows that with an increase in the generation frequency, the current becomes more symmetrical and its constant component, which is responsible for undesirable destructive processes of electrolysis in the electrolyte matrix of  
Figure 3: Graphs of current (red curve) and voltage (yellow curve) in the active electrode (AE)–electrolyte-neutral electrode (NE) circuit.  
Figure 2: Stand for the study of LTP generation: (**a**) photo of the stand; (**b**) the main functional elements of the stand—a cell with an electrolyte solution (1), an oscilloscope (2), a high-frequency generator (G-HF) (3). Current and voltage oscillograms in the active electrode (AE)–electrolyte-neutral electrode (NE) circuit during the generation of LTP discharges were recorded using an electronic oscilloscope; (**c**) plasma photograph on the surface of a needle electrode with a diameter of 300 \(\upmu\)m.  
the tissue, decreases. An increase in the current frequency to 6.78 MHz leads to the almost complete disappearance of the constant component, a decrease in the plasma discharge initiation voltage, and stabilization of their formation. The noted facts indicate that the current frequency of 6.78 MHz is more effective in terms of reducing the destructive effect of electrolysis processes. In addition, thermal damage to tissues in the form of thermal coagulation is less pronounced at high frequencies. Increasing the exposure time at low frequencies only increases the thermal damage to the tissue. The use of higher frequencies caused difficulties in supplying current to the monopolar electrode.  
From the point of view of the efficiency of proliferation, a further increase in the frequency of generation is not advisable. This is because the frequency of the ion current above 10 MHz ceases to influence the formation of the transfer wave of calcium ions involved in the processes of ion transport inside the cell [37; 38]. A further increase in the frequency of the ion current begins to block the transport of calcium ions in cells [36; 38]. In this regard, the LTP generation frequency of 6.78 MHz also falls into the region of optimal exposure parameters. Clinical studies carried out in the next part of the work confirmed the benefits of activating reparative processes in the tissues of the problem area by using a current with a frequency of 6.78 MHz.