# Rapid Molecular Identification of Scolytinae (_Coleopterara: Curculionidae_)
## 2 Results
### Primer Pairs for Molecular Identification of Diverse Genera
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & \multicolumn{4}{c}{**EF-1a (efalafor1/efarev1)**} \\ \hline \multirow{2}{*}{**Sample**} & \multirow{2}{*}{**Genus**} & **PCR** & \multirow{2}{*}{**Accession**} & \multirow{2}{*}{**\% Identical**} \\  & & **Concentration** & & & & \\  & & **(ng/uL)** & & & \\ \hline B2 & _Coptoborus pseudotenuis_ & 4.37 & _Coptoborus pseudotenuis_ & AF508880 & 99.7\% \\ B4 & _C. pseudotenuis_ & 4.36 & _Coptoborus pseudotenuis_ & AF508880 & 99.7\% \\ B6 & _C. pseudotenuis_ & & No amplification & & \\ B7A & _C. pseudotenuis_ & 11 & _Coptoborus pseudotenuis_ & AF508880 & 100.0\% \\ B7B & _C. pseudotenuis_ & 5.1 & _Coptoborus pseudotenuis_ & AF508880 & 100.0\% \\ \hline B3 & _Xyleborus ferrugineus_ & 2.92 & _Xyleborus ferrugineus_ & KP941383 & 100.0\% \\ \hline B14 & _Theoborus_ sp. & 15.8 & _Theoborus_ sp. & HM064194 & 99.6\% \\ BC1 & _Theoborus_ sp. & 10.2 & _Theoborus_ sp. & HM064194 & 99.7\% \\ BC9 & _Theoborus_ sp. & 14.1 & _Theoborus_ sp. & HM064194 & 99.6\% \\ BC13 & _Theoborus_ sp. & 19 & _Theoborus_ sp. & HM064194 & 99.7\% \\ \hline B16 & _Hypothenemus_ sp. & 10.3 & _Hypothenemus_ _nr. eduitus_ & JX264092 & 97.0\% \\ B17 & _Hypothenemus_ sp. & & No amplification & & \\ B19 & _Hypothenemus_ sp. & 16.1 & _H. nr. eruditus_ & JX264092 & 97.7\% \\ B20 & _Hypothenemus_ sp. & 5 & _Hypothenemus_ sp. & AF186658 & 96.0\% \\ \hline J1 & _Araptus_ sp. & & No amplification & & \\ J2 & _Araptus_ sp. & 11.2 & _Phelleterus_ sp. & KY805860 & 91.6\% \\ J4 & _Araptus_ sp. & 7.36 & _Phelleterus_ sp. & KY805860 & 91.6\% \\ \hline \hline \end{tabular}
\end{table} 
Table 4: BLASTn results for EF-1a sequences amplified and sequenced with primers efalafor1 and efarev1. Concentration of PCR product sent for sequencing also shown to the left of the GenBank match. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & \multicolumn{4}{c}{**EF-1a (ets149/efa754)**} \\ \hline \multirow{2}{*}{**Sample**} & \multirow{2}{*}{**Genus**} & **PCR** & \multirow{2}{*}{**Accession**} & \multirow{2}{*}{**\% Identical**} \\  & & **Concentration** & & & \\  & & **(ng/uL)** & & & \\ \hline B2 & _Coptoborus pseudotenuis_ & 12.9 & _Coptoborus pseudotenuis_ & AF508880 & 99.7\% \\ B4 & _C. pseudotenuis_ & 12.4 & _Coptoborus pseudotenuis_ & AF508880 & 99.0\% \\ B6 & _C. pseudotenuis_ & & No amplification & & \\ B7A & _C. pseudotenuis_ & 16.3 & _Coptoborus pseudotenuis_ & AF508880 & 100.0\% \\ B7b & _C. pseudotenuis_ & 5.1 & _Coptoborus pseudotenuis_ & AF508880 & 100.0\% \\ \hline B3 & _Xyleborus ferrugineus_ & 38.9 & _Xyleborus ferrugineus_ & KP941383 & 94.7\% \\ \hline B14 & _Theoborus_ sp. & 9.53 & _Theoborus_ hepromae & AF259881 & 99.6\% \\ BC1 & _Theoborus_ sp. & 10.2 & _Theoborus_ sp. & HM064194 & 99.7\% \\ BC9 & _Theoborus_ sp. & 5.56 & Poor sequence & & \\ BC13 & _Theoborus_ sp. & 9.05 & _Theoborus_ theoborus_ & AF259881 & 100.0\% \\ \hline B16 & _Hypothenemus_ sp. & 25.9 & _Hypothenemus_ _nr. eduitus_ & JX264092 & 98.1\% \\ B17 & _Hypothenemus_ sp. & & No amplification & & \\ B19 & _Hypothenemus_ sp. & 25.9 & _Hypothenemus_ _nr. eduitus_ & JX264092 & 99.3\% \\ B20 & _Hypothenemus_ sp. & 20.4 & Poor sequence & & \\ \hline J1 & _Araptus_ sp. & 7.62 & Poor sequence & & \\ J2 & _Araptus_ sp. & 38.2 & Poor sequence & & \\ J4 & _Araptus_ sp. & 14.9 & Poor sequence & & \\ \hline \hline \end{tabular}
\end{table} 
Table 5: BLASTn results for EF-1a sequences amplified and sequenced with primers ef149 and efa754. Concentration of PCR product sent for sequencing also shown to the left of the GenBank match. which had a maximum % identity of 85.5% (Table 7). All sequences generated in this study are in Table S3.