## 3 Results
### Blind Docking Results
\begin{table}
\begin{tabular}{c c c c} \hline
**Terpene** & **Enzyme** & **Interaction** & **Match** \\ \hline \multirow{4}{*}{Limonene} & \multirow{2}{*}{COX-1} & PRO128, ARG376, PRO125, ALA378, ILE124, ILE377, & \multirow{2}{*}{PHE381} \\  & & PHE529, PHE381, LY5532 & \\ \cline{2-3}  & \multirow{2}{*}{COX-2} & PRO125, PRO128, ARG376, ILE124, ILE377, PHE529, & \multirow{2}{*}{PHE381} \\  & & LY5532, ALA378, PHE381 & \\ \cline{2-3}  & 5-LOX & LEU448, TYR470, ILE454, PHE450, VAL243, ALA453 & No match \\ \cline{2-3}  & \multirow{2}{*}{PLA\({}_{2}\)} & PHE63, LY562, PHE63 & No match \\ \hline \multirow{4}{*}{Camphor} & COX-1 & ALA202, LEU390, MET391, THR206, HIS388 & HIS388 \\ \cline{2-3}  & COX-2 & ALA202, HIS388, THR206, MET391, LEU390 & No match \\ \cline{2-3}  & 5-LOX & HIS130, VAL110, VAL109, ARG101, LY5133 & No match \\ \cline{2-3}  & PLA\({}_{2}\) & LY562, LEU64, PHE63, PHE63 & No match \\ \hline \multirow{4}{*}{Linalool} & COX-1 & HIS388, HIS386, LEU390, TYR385, ALA202, PHE21, & HIS388, TYR385 \\ \cline{2-3}  & COX-2 & LEU390, MET391, ALA202, HIS388, HIS207, PHE210, & \multirow{4}{*}{TYR385} \\  & & HIS386, THR206, TYR385 & \\ \cline{2-3}  & \multirow{2}{*}{5-LOX} & PHE359, PHE412, LEU414, TRP599, VAL604, HIS600, & LEU414, TRP599, ALA603, \\ \cline{2-3}  & ALA\({}_{2}\) & ALA603, TYR181, LEU420, ALA424 & \multirow{2}{*}{TYR181} \\ \cline{2-3}  & PLA\({}_{2}\) & LY562, LEU64, LEU64, PHE63 & No match \\ \hline \multirow{4}{*}{Menthol} & COX-1 & HIS388, TYR385, ALA202, MET391, LEU390, ALA199 & HIS388, TYR385 \\ \cline{2-3}  & COX-2 & HIS388, TYR385, ALA202, MET391, LEU390, ALA199 & TYR385 \\ \cline{2-3}  & 5-LOX & PHE544, PHE450, SER447, ARG370, ALA453, VAL243 & No match \\ \cline{2-3}  & PLA\({}_{2}\) & LEU64, THR61, LY562, PHE63, PHE63 & No match \\ \hline \multirow{4}{*}{\(\alpha\)-Pineine} & COX-1 & TYR385, LEU390, HIS388, MET391, ALA199, & \multirow{4}{*}{TYR385, HIS388, TRP387} \\  & \multirow{2}{*}{COX-2} & ALA202, TRP387 & \\ \cline{2-3}  & \multirow{2}{*}{COX-2} & ALA199, HIS388, TYR385, TRP387, LEU390, & \multirow{2}{*}{TYR385, TRP387} \\  & & MET391, ALA202 & \\ \cline{2-3}  & 5-LOX & VAL645, LEU641, PRO664, LEU617, MET619, ARG638 & No match \\ \cline{2-3}  & PLA\({}_{2}\) & PHE63, THR61, PHE63, LY562, LEU64 & No match \\ \hline \multirow{4}{*}{\(\beta\)-Pineine} & COX-1 & HIS388, LEU390, TYR385, TRP387, ALA202, MET391 & TYR385, HIS388, TRP387 \\ \cline{2-3}  & COX-2 & HIS388, LEU390, TYR385, TRP387, ALA202, MET391 & TYR385, TRP387 \\ \cline{2-3}  & 5-LOX & LEU641, LEU617, VAL645, PRO664, MET619, ARG638 & No match \\ \cline{2-3}  & PLA\({}_{2}\) & PHE98, PHE5, ALA94, HIS47, CYS28, CYS44 & PHE5, CYS44, HIS47 \\ \hline \end{tabular}  
* AA is not included in this residue-level interaction table, as its docking poses do not reliably align with the catalytically important residues under the rigid-body blind docking conditions.  
\end{table} 
Table 6: Interaction profile of selected terpenes and arachidonic acid (AA) with active sites of COX-1, COX-2, 5-LOX, and PLA\({}_{2}\) enzymes. COX-1 is a constitutively expressed enzyme involved in the synthesis of prostaglandamins essential for maintaining gastrointestinal and renal homeostasis. The analysis reveals that key contact residues within the active site include LEU117, ARG120, PHE205, PHE209, TYR348, PHE381, TYR385, and HIS388. Limonene forms a stable contact with PHE381, a residue within the active site. Camphor, linalool, menthol, \(\alpha\)-pinene, and \(\beta\)-pinene exhibit strong interactions with HIS388 and TYR385, which may contribute to their affinity for the enzyme. Additionally, \(\alpha\)-pinene and \(\beta\)-pinene interact with TRP387, which could play a stabilizing role in ligand binding. A representative 2D interaction map (Figure 5) is generated to visualize the binding interactions between each terpene and the COX-1 active site.