## 2 Materials and Methods
### Experimental Setup  
A schematic figure of the experimental setup is shown in Figure 2. A commercial aerosol generator (model ATM228, Topas GmbH, Dresden, Germany) was used to generate positive reference material at 800 hPa and sodium chloride (NaCl) aerosol particles from a 5 wt% solution prepared in deionized water. The NaCl droplets were dried by using a silica dryer. Infectious aerosols are suspensions of small organisms in the form of particles in the air. The size of the particle is the most important decisive factor for aerosol behavior. Particles that are 2 \(\upmu\)m or smaller in size can remain airborne in most indoor environments unless there is removal by air filtration.  
Two groups of 10 collectors (MF-MilliporeTM Membrane Filter VSWP02500, Merck, Darmstadt, Germany) were set up with/without protection tents. Each collector performed three intubation simulations with no protection tent and three with a protection tent. In all the tests, the L-shaped frames that were originally placed were not moved. An easy, highly efficient, and sensitive method for the monitoring of airborne pathogenic microorganisms and viruses is the mixed cellulose ester membrane filter method [24]. The suitability of water-soluble filters, especially for virus sampling, has already been tested and proven. These filters are particularly suitable because they show excellent virus collection efficiency  
\begin{table}
\begin{tabular}{c c c} \hline
**Primers** & **Sequence (5\({}^{\prime}\)-3\({}^{\prime}\))** & **Annealing Temperature** \\ \hline E\_Sarbeco\_F1 & 5\({}^{\prime}\)-ACAGGTACGTTAATAGTTAATAGCGT-3\({}^{\prime}\) & \\ E\_Sarbeco\_R2 & 5\({}^{\prime}\)-ATATTGCACGACTACGCAC  
with high retention rates. As soon as the filter has been dissolved in deionized water, or any other appropriate buffer or medium, all the viral particles retained on the filter can be further processed and detected using RT-qPCR.  
Even sensitive RNA material can be detected by a combination of gelatin filtration and RT-qPCR. However, it should be considered that RNA extraction is a crucial step within sample preparation. Therefore, to reduce the loss of genetic material, attention should be given to this step. Several studies have shown that RT-qPCR analysis after air sampling using filters provides recovery results superior to those obtained with other detection methods and hence can be considered a precise and practicable method for the detection of airborne viruses [25; 26].  
With the recognition that the procedures for generated aerosol are of particular importance to practice in situations with a high risk of infection, an additional tool for intubation training that helped in focusing knowledge and motivating changes to practice in hospitals to use the protective tent was tested. The Glo Germ(tm) (Marlatek Inc., Brockville, ON, Canada) was used as the UV fluorescent solution in these simulations, which is 5-10 microns in size. A simulated study was conducted to explore the relationship between protective tent usage during intubation and contamination by medical personnel pre-dofing and post-dofing PPE. This study tested video laryngoscope-assisted intubation on an intubation mannequin with and without a protection tent in a random order.