## 3 Results and Discussion
### Evaluation of the Effect of L-Methioninase on Migration Characteristics of Glioblastoma Cells  
To evaluate the migratory capabilities of glioblastoma cells in response to L-methionineinase, a wound-healing test was performed. L-methioninease application successfully inhibited the migration of U87 MG and T98G cells.  
For U87 MG cells, the photographs taken at 0, 8, and 24 h clearly show that, in the control group, the cells migrated and closed the wound within 24 h. However, in the presence of L-methioninease and etoposide, cell migration was inhibited, and the wound remained open even after 24 h (Figure 7). For T98G cells, the photographs taken at 0, 8, and 24 h clearly show that, in the control group, the cells migrated and closed the wound within 24 h. Similarly, in the presence of L-methioninease and etoposide, cell migration was inhibited, and the wound remained open even after 24 h (Figure 8). These results indicate that L-methioninease can not only induce cytotoxicity against U87 MG and T98G cells but also inhibit their migration, which is an important aspect in the treatment of cancer as it can help prevent the dissemination of cancer cells and the formation of metastases.  
The migration assay was performed by calculating the wound area and average wound width using ImageJ software. For U87 MG cells, the graphs of wound area and average wound width change clearly demonstrate that the presence of L-methioninease, etoposide, and their combination inhibited wound closure over a 24 h period (Figures 9 and 10). A slight increase in the use of both L-methioninease and etoposide, which are cytotoxicity powerful, started to kill some cells after 24 h of incubation.  
Figure 6: Cytotoxicity of L-methioninease, etoposide and combination of both on MEF, HaCat, U87 MG and T98G cells for 24 h of incubation (\(p\) < 0.001).  
Figure 8: The wound closure status of T98G cells over 24 h. L-methionineinase and etoposide inhibited the wound closure with both sole and combined administration. In control plates, glioblastoma T98G cells closed the wound in 24 h of incubation (\(p<0.001\)).  
Figure 7: Wound closure status of U87 MG cells over 24 h. L-methionineinase and etoposide inhibited the wound closure with both sole and combined administration. In control plates, glioblastoma U97 MG cells closed the wound in 24 h of incubation (\(p<0.001\)).  
In the control group, cell migration appeared to close the wounds of U87 MG cells. However, negative cell migration and wound closure percentages were observed in the experimental groups treated with etoposide and L-methionineinase (Figure 11). This observation suggests that the treatments might have resulted in cell death, leading to a decrease in the number of migrating cells, which could have been reopened because of the high cytotoxic effects of L-methioninease and etoposide, which inhibit wound closure.  
Figure 10: Average wound width change in U87 MG during 24 h incubation.  
Figure 9: Wound area change in U87 MG cells for 24 h incubation.  
Figure 11: Cell migration rate over 24 h of incubation in U87 MG cells.  
Figure 12: Wound area change in T98G cells for 24 h incubation.