## 3 Results
### Functional Connectivity  
To provide a detailed account of the brain-wide connectivity of the subjects, we analyzed the functional connectivity of the 47 channels (with a threshold value of 0.9) (Figure 5). Figure 5a-c demonstrates the results of the functional connectivity assessment for each phase of the CG. Functional connectivity tended to decrease and change over time in the CG. Functional connections were connected to a large brain region in the first phase (0 weeks), including the PFC, SMA, and M1 regions in the CG (Figure 5a). Functional connections decreased after 4 weeks compared with the first phase, particularly for the M1 and SMA regions (Figure 5b). Functional connections in the last phase were slightly increased in the M1 region compared to the second phase (Figure 5c). The functional connectivity results of the IG are shown in Figure 5d-f. Functional connectivity was shown to be weak in the M1 and PFC regions associated with gait in the first phase (Figure 5d). After 4 weeks of acupuncture treatment, the functional connectivity decreased in the PFC region; on the other hand, functional connectivity between M1 regions increased (Figure 5e). Figure 5f shows the results of the 4-week follow-up after the treatment with acupuncture. The results show that in contrast to the observations immediately following treatment, the connectivity of the M1 region had decreased, while the connectivity of the PFC region had increased.  
\begin{table}
\begin{tabular}{c c c c c c} \hline  & & **CG** & **IG** & **_t-Value_ & _p-Value_ \\ \cline{3-6}  & & **Mean \(\pm\) SD (mM)** & **Mean \(\pm\) SD (mM)** & & \\ \hline \multirow{4}{*}{**M1**} & **V1** & 0.498 \(\pm\) 0.00008 & 0.58 \(\pm\) 0.00007 & \(-\)0.753 & 0.4542 \\ \cline{2-6}  & **V4** & 0.433 \(\pm\) 0.00004 & 0.419 \(\pm\) 0.00003 & 0.267 & 6.54 \(\times\) 10\({}^{-2}\) \\ \cline{2-6}  & **V8** & 0.658 \(\pm\) 0.00011 & 0.477 \(\pm\) 0.00004 & 1.701 & 9.32 \(\times\) 10\({}^{-4}\) *** \\ \hline \multirow{4}{*}{**SMA**} & **V1** & 0.498 \(\pm\) 0.00008 & 0.705 \(\pm\) 0.00008 & \(-\)1.872 & 0.7898 \\ \cline{2-6}  & **V4** & 0.423 \(\pm\) 0.00004 & 0.587 \(\pm\) 0.00004 & \(-\)3.105 & 2.20 \(\times\) 10\({}^{-3}\) ** \\ \cline{2-6}  & **V8** & 0.453 \(\pm\) 0.00005 & 0.568 \(\pm\) 0.00005 & \(-\)1.635 & 4.75 \(\times\) 10\({}^{-6}\) *** \\ \hline \multirow{4}{*}{**PFC**} & **V1** & 0.569 \(\pm\) 0.00008 & 1.02 \(\pm\) 0.0001 & \(-\)3.457 & 9.08 \(\times\) 10\({}^{-2}\) \\ \cline{2-6}  & **V4** & 0.489 \(\pm\) 0.00004 & 0.773 \(\pm\) 0.00005 & \(-\)4.731 & 0.104 \\ \cline{1-1} \cline{2-6}  & **V8** & 0.482 \(\pm\) 0.00006 & 0.667 \(\pm\) 0.00005 & \(-\)2.376 & 1.86 \(\times\) 10\({}^{-2}\) * \\ \hline \end{tabular} Values are mean \(\pm\) standard deviation; * \(p\) \(<\) 0.05, **_p_ \(<\) 0.01, ***_p_ \(<\) 0.001.  
\end{table}
Table 4: Statistical results of peak values in HbO.  
Figure 5: Illustrations of functional connectivity network patterns of ROIs before, after a 4-week course of acupuncture treatment, and at 4-week follow-up of PD patients. (**a**) First phase (0 weeks) in the CG, (**b**) second phase (4 weeks) in the CG, (**c**) third phase (8 weeks) in the CG, (**d**) before treatment (0 weeks) in the IG, (**e**) after a 4-week course of acupuncture treatment in the IG, (**f**) after 4-week follow-up in the IG.