## 2 Results and Discussion
### WM793 Human Melanoma Line  
When analyzing the effect of different extracts obtained from cookies with the addition of selected wild-grown fruits on their anticancer properties against human skin cancer (melanoma) WM793 cells, statistically significant differences were observed between the mean values of cytotoxicity and viability (\(p<0.05\)) (Table 2). Cookies' extracts containing sea buckthorn berries were the most effective in defending WM793 melanoma cells at three concentrations added (0.5 mg/mL; 1.5 mg/mL, and 2.5 mg/mL) (\(p<0.05\)) (Figure 1). This results in 89.95% cytotoxicity and the lowest viability (45.93%) at the highest concentration applied (2.5 mg/mL) (Table 2, Figure 1). The inhibiting potential of cancer cell growth upon lower concentrations of sea-buckthorn extracts was 0.5 mg/mL--27% cytotoxicity and 76% viability; 1.5 mg/mL--26% cytotoxic force and 70% viability (Table 2). The remaining extracts containing other berries were definitely less efficient (\(p<0.05\)) than the fruits of _Hippocampae rhamnoides_ L. depending on the dose used (Figure 1). Elderlyberry showed the greatest cytotoxicity (3.84%) potential at the highest concentration (2.5 mg/mL) while cookies baked with the addition of hawthorn fruits showed the highest activity at a concentration of 1 mg/mL--5.19% and with the addition of chokeberry fruit at 1.5 mg/mL--1.99% (Table 2).  
\begin{table}
\begin{tabular}{l c c c c c} \hline  & & \multicolumn{4}{c}{**Dough Ingredients [g]**} \\ \cline{2-6}
**Cookies Kinds** & **Wheat** & **Margarine** & **Saccharose** & **Eggs** & **Baking** & **Lyophilized** \\  & **F flour** & **Margarine** & **Saccharose** & **Eggs** & **Powder** & **Fruits** \\ \hline Control & 500 & 250 & 200 & 150 & 5 & 0 \\ Chokeberry (_Aronia melanocarpa_) & 495 & 250 & 200 & 150 & 5 & 5 \\ Elderberry (_Sambucus nigra_ L.) & 495 & 250 & 200 & 150 & 5 & 5 \\ Hawthorn (_Cratageus_ L.) & 495 & 250 & 200 & 150 & 5 & 5 \\ Sea-buckthorn (_Hippocampae rhamnoides_ L.) & 495 & 250 & 200 & 150 & 5 & 5 \\ \hline \end{tabular}
\end{table}
Table 1: The recipe formulation of different kinds of cookies.  
\begin{table}
\begin{tabular}{c c c} \hline  & **Cytotoxicity 1** & **Viability 1** \\ \hline
**Extract Type** & **Extract Concentration** & **Mean Value [\%] \(\pm\) Standard Deviation** \\  & **[mg/mL]** & **Mean Value [\%] \(\pm\) 5.19\%** & **Standard Deviation** \\ \hline  & 0.5 & 27.23 b \({}^{\text{h}}\) \(\pm\) 0.02 & 75.72 b\({}^{\text{h}}\)\(\pm\) 0.02 \\ Sea-buckthorn & 1.0 & 3.71 d \({}^{\text{h}}\)\(\pm\) 0.06 & 92.84 b\({}^{\text{h}}\)\(\pm\) 0.04 \\ (_Hippocampae rhamnoides_ L.) & 1.5 & 25.59 b\({}^{\text{h}}\)\(\pm\) 0.03 & 70.00 d\({}^{\text{h}}\)\(\pm\) 0.06 \\  & 2.5 & 89.85 c \({}^{\text{h}}\)\(\pm\) 0.00 & 34.02 e\({}^{\text{h}}\)\(\pm\) 0.02 \\ \hline  & \(x\) & 36.60 & 68.15 \\ \hline  & 0.5 & 0.00 & 100.00 d \(\pm\) 0.05 \\ Hawthorn & 1.0 & 5.19 a \({}^{\text{h}}\)\(\pm\) 0.00 & 100.00 d \(\pm\) 0.07 \\ (_Cratageus_ L.) & 1.5 & 1.99 c \({}^{\text{h}}\)\(\pm\) 0.01 & 94.29 a\({}^{\text{h}}\)\(\pm\) 0.08 \\  & 2.5 & 3.02 d \({}^{\text{h}}\)\(\pm\) 0.00 & 100.00 d \(\pm\) 0.04 \\ \hline  & \(x\) & 2.51 & 100 \\ \hline  & 0.5 & 1.83 a \({}^{\text{h}}\)\(\pm\) 0.02 & 92.60 a\({}^{\text{h}}\)\(\pm\) 0.06 \\ Elderberry & 1.0 & 1.54 a\({}^{\text{h}}\)\(\pm\) 0.01 & 97.94 a\({}^{\text{h}}\)\(\pm\) 0.09 \\ (_Sambucus nigra_ L.) & 1.5 & 1.17 c \({}^{\text{h}}\)\(\pm\) 0.01 & 97.91 a\({}^{\text{h}}\)\(\pm\) 0.04 \\  & 2.5 & 3.84 e\({}^{\text{h}}\)\(\pm\) 0.01 & 100.00 a\({}^{\text{h}}\)\(\pm\) 0.06 \\ \hline \end{tabular}
\end{table}
Table 2: Cytotoxicity assay after 24 h incubation of extracts with different wild-grown fruits enriched cookies on WM793 human melanoma cell line.