## 3 Results and Discussion
### Sec-Malls Analysis of Saccharide Fractions  
The five fractions were analyzed using SEC-MALLS and the results are summarized in Table 2. The average molecular weight (M\({}_{\rm w}\)) ranged from 3 kDa for oligoalginates OASM obtained from ALSM to 2173 kDa for sulfated arabinolactans extracted from the green seaweed _C. decorticatum_ (Table 2). The polydispersity index (D) was less than 2 for the \begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline \multirow{2}{*}{**Fractions**} & \multirow{2}{*}{**Source**} & **Yield** & **Total Sugar** & **Neutral** & **Uronic Acid** & **Sulfate** & **Protein** & **Polyphenol** \\  & & **(\% _wt/w_)** & **(\% _wt/w_)** & **(\% _wt/w_)** & **(\% _wt/w_)** & **(\% _wt/w_)** & **(\% _wt/w_)** \\ \hline ALSM \({}^{1}\) & _S. muticum_ & 23 & 44.46 \(\pm\) 1.67 & 2.42 \(\pm\)1.96 & 63.27 \(\pm\) 0.17 & Nd \({}^{6}\) & Trace & Nd \\ ALCM \({}^{2}\) & _C. myriophylloides_ & 31 & 57.48 \(\pm\) 0.35 & 30.27 \(\pm\) 0.84 & 44.99 \(\pm\) 3.70 & 7.29 \(\pm\)0.4 & Nd & Nd \\ FUCCM \({}^{3}\) & _C. myriophylloides_ & 0.87 & 52.11 \(\pm\) 3.07 & 29.06 \(\pm\) 0.52 & 23.19 \(\pm\) 1.56 & 12.83 \(\pm\) 0.06 & 2.93 \(\pm\)0.17 & Nd \\ AGB \({}^{4}\) & _C. decorticatum_ & 11 & 37.67 \(\pm\) 1.10 & 37.56 \(\pm\) 1.73 & 3.05 \(\pm\) 0.29 & 22.68 \(\pm\) 2.08 & 20.03 \(\pm\) 0.29 & Trace \({}^{7}\) \\ AGP \({}^{5}\) & _C. decorticatum_ & 5 & 48.38 \(\pm\) 1.44 & 45.68 \(\pm\) 0.58 & 4.04 \(\pm\) 0.35 & 13.21 \(\pm\) 0.23 & 11.89 \(\pm\) 2.48 & Trace \\ \hline \hline \end{tabular}  
* ALSM: alginate extracted from SM; \({}^{2}\) ALCM: alginate extracted from CM; \({}^{3}\) FUCCM: fucoidan extracted from CM; \({}^{4}\) AGB: sulfated glycoprotein (crude extract) extracted from CD; \({}^{5}\) AGP: sulfated glycoprotein (purified extract) extracted from CD; \({}^{8}\) Nd: Not determined; \({}^{7}\) Trace: % \(w/w<1\).  
\end{table} 
Table 1: Biochemical characterization and extraction yield of polysaccharides extracted from CE, SM, and CM. ALCM (1.3), ALSM (1.2), OACM (1.7), OASM (1.04), and AGP (1.6) fractions, and greater than 2 for the AGB (3.1) and FUCCM (4) fractions. These indicate that the purified arabinogradancans (AGP) and ablginates polymers and oligomers are monodisperse with minor degradation or contamination with fucoidans in the case of ALSM and ALCM extracts. For the crude extract (AGB) and FUCCM, the D values were higher, meaning that both are polydisperse polymers presenting impurities of other poly/oligosaccharides such as laminin and especially alginate for FUCCM. The intrinsic viscosity of ALSM and AGP presented the highest averages, whereas the oligoalginates (OASM) exhibited the lowest values compared to other fractions. Usually, the intrinsic viscosity of polysaccharides is dependent on their molecular weight (M\({}_{\text{w}}\)) and chain dimension, which explains the fluctuation of values of alginates and their derivatives [52]. It was found that the presence of salts in the solution could also decrease the [n], which exhibited a typical polyelectrolyte behavior [53]. This might explain the low intrinsic viscosity of AGB and AGP despite their important M\({}_{\text{w}}\). In addition, FUCCM presented an important M\({}_{\text{w}}\) of 148 kDa, compared to ALCM (76 kDa). However, the [n] was lower (84.4 mL/g) than that found in ALCM (224.2 mL/g). These indicated that the presence of sulfates in polysaccharides could decrease the [n] values due to the charge screening effects, leading to a more compact structure, hence, reducing the hydrodynamic volume of the molecule [54]. The fractions of OACM and OASM were obtained after 6 h of radical depolymerization of ALCM and ALSM, respectively. The M\({}_{\text{w}}\) exhibited an important fluctuation in which OASM is characterized by a low M\({}_{\text{w}}\) (3 kDa) and DP of 14 compared to the OACM fraction (M\({}_{\text{w}}\) = 19 kDa and DP = 88). These indicate that the OASM was hydrolyzed into oligomers in which the DP of ALSM was sharply decreased from 935 to 14, contrary to OACM.