## 3 Results and Discussion
### Search for Resistance and Virulence Genes in Reference Genomes
You can see colored squares with gradients of tones representing the number of resistance or virulence genes, according to intensity, in the genome. Furthermore, the genes identified confer resistance to 28 antibiotics. The 3 main classes were fluoroquinolones, tetracyclines, and peptide antibiotics and disinfect agents, with 28, 21, and 19 resistance genes, respectively. In a study conducted by [PERSON] et al. (2021), isolated strains of _S. chromogenes_ showed the highest resistance to penicillin (95%), tetracycline (86%), and oxacillin (81%). Furthermore, through phenotypic manifestations and detection of the presence of the _mecA_ gene, it was possible to verify that 30% of the isolates were resistant to methicillin. Additionally, 62% of the isolates were multidrug resistant. According to Figure 10A, reference genomes of species detected in the BPP group presented a wide variety of mechanisms and genes, with a vast predominance focused on antibiotic efflux (n = 60) (_Abua_AbaQ_, _abeM_, _acP_, _aacP_, _adeJ_, _adeJ_, _atel_, _cpxA_, _CRP_, _Eclo_acrA_, _Ecol_mdfA_, _emrR_, _floR_, _H-NS_, _kldpE_, _Kpne_KpnE_, _Kpne_KpnF_, _Kpne_KpnG_, _Kpne_KpnH_, _leuO_, _LptD_, _marA_, _mdtA_, _mdtB_, _mdtC_, _mdtH_, _MdtK_, _MexB_, _MexE_, _MexK_, _MexK_, _MexK_, _MexN_, _mgrA_, _mshA_, _norc_, _OpmH_, _OrrM_, _OrrN_, _oaxA_, _oaxB_, _Paer_Cpx_, _Park_, _PmpM_, _ramA_, _RanA_, _RanB_, _rosA_, _rosB_, _rsmA_, _Saur_nor_, \(A\), _sepA_, _tetA(9)_, _TolC_, _Tric_, _YajC_, and _YojI_). The families of efflux pumps, as they are called, are located in the plasma membrane and use energy in different ways to translocate substances from the cells' internal environment to the external environment and vice versa, playing a crucial role in the resistance of these cells bacteria [108]. They are often found conserved, suggesting maintenance of this type of antimicrobial resistance in evolution and consequently conferring some resistance against B-lactams, tetracyclines, macrolides, and quinolones, among others [109; 110]. A second important group of genes found is resistance genes focused on altering the antibiotic target (_ArnT_, _bacA_, _eptA_, _eptB_, _PmrF_, _sul2_, and _ugd_) (Figure 10A). These types of resistance mechanisms can be considered central in this process since many antibiotics have a high affinity for growth inhibition or death, only with their primary target, and any change in this target makes the inhibitory compound inefficient, resulting in new resistance mechanisms [111]. Finally, a third large group of genes found in our analysis is related to antibiotic inactivation (_APH(3\({}^{\prime\prime}\))-lb_, _APH(6)-ld_, _FosA6_, _ORN-1_, _OXA-296_, and _Tet(X3)_). This type of mechanism can damage or degrade the medicine and consequently reduce its efficiency. Through hydrolysis of the amide bond with the B-lactam ring or even by binding other tetracycline hydroxylase enzymes, these genes confer resistance to several classes, such as carbapenemems and cephalosporins [111]. Considering the reference genome of the only species exclusive to the BHMT group, _Macrococcus caseolyticus_ FDAARGOS 868, we can see the presence of a single _nor_C gene with antibiotic efflux function. The species that presented the highest number of resistance genes were _Raoultella ornithinolytica_ NCTC9164 with 41 genes, with the functions of efflux (n = 31), alteration of the target of action (n = 6), inactivation (n = 2), and reduction in permeability (n = 4), followed by _Pantoea agglomerans_ FDAARGOS 1447 with 23 resistance genes (efflux--n = 20, alteration of the target of action--n = 2, and reduction in permeability--n = 1) (Figure 10A). The genus _Pseudomonas_ also presented a significant number of genes (n = 39) divided into 3 species: _P. fluorescens_ATCC_13525 (n = 15), _P. weihenstephanensis_ TMW2 1728 (n = 11), and _P. lundensis_2T_2\(5\)2 (n = 13). Interestingly, a similar profile was found between the genes in the species of this genus.