## 3 Results
### Validation of Genes Related to Calcium Signaling Pathway  
The expression levels of 12 genes involved in the calcium signaling pathway were also verified (Figure 6). Transcription of most genes in the leaves correlated well with the digital genes' expression detected by RNA sequencing (Tables S3 and S4). In the leaves, the expression of _calcium-dependent protein kinase 26_ (_CDPK26_) kept on increasing until 4 h after treatment, followed by a significant decrease. Transcription of _calcium-binding protein CML35_ (_CML35_) showed an increase after treatment for 1 h and remained relatively stable at 4 h, followed by an obvious increase again. The expression of _CBL-interacting serine/threonine-protein kinase 4_ (_CIPK4_), _potassium channel AKT2_ (_AKT2_), _NADH/NADPH oxidase_ increased to a high level after treatment for 1 h and decreased ever since. _Calcineurin B-like protein 3_ (_CBL3_) and potassium channel _KAT1_ (_KAT1_) were highly expressed in the plants without treatment and lowly expressed after treatment for 4 h. Transcription of _potassium channel AKT1-like_ (_AKT1_) underwent a continuous decline during the treatment process. _Calmodulin 6_ (_CaM6_), _mitogen-activated protein kinase 15_ (_MAPK15_), _mitogen-activated protein kinase kinase 2_ (_MAP2K2_), and _mitogen-activated protein kinase kinase 1_ (_MAP3K1_) all showed a similar expression pattern. In the cloves, transcription of _CDPK26_ and _AKT2_ decreased until 4 h after treatment, followed by an evident increase. The expression of _CML35_ was downregulated after salt treatment and remained at a stable level with time extending. _CIPK4_ and _CBL3_ were lowly expressed after treatment for 12 h, whereas _KAT1_ showed the lowest expression at 4 h. The transcript levels of _AKT1_, _CML35_, _NADH/NADPH oxidase_, _MAP2K2_, and _MAP3K1_ showed an increase initially, followed by a continuous decrease since. No obvious difference was detected in _CaM6_ expression. In the roots, _CDPK26_ and _MAP2K2_ were lowly expressed after treatment for 1 h, whereas _CIPK4_ and _NADH/NADPH oxidase_ showed the lowest mRNA abundance at 12 h after treatment. _CML35_ and _AKT2_ possessed a similar expression pattern. Transcription of _KAT1_, _AKT1_, and _MAP3K1_ increased to a high level in the early process of salt treatment, followed by a continuous decline. _CML6_ and _MAPK15_ kept relatively stable expression until 4 h after treatment (Figure 6).  
Figure 6: Expression patterns of genes involved in calcium signaling in garlic samples subjected to drought stress. Genes involved in calcium signaling include (**a**) calcium-dependent protein kinase 26  
(Unigene30812_All), (**b**) calcium-binding protein CML35 (CL8214.Contig1_All), (**c**) CBL-interacting serine/threonine-protein kinase 4, (**d**) calcineurin B-like protein 3 (CL3180.Contig2_All), (**e**) potassium channel KAT1 (Unigene25878_All), (**f**) potassium channel AKT1-like (CL2213.Contig1_All), (**g**) potassium channel AKT2 (Unigene12039_All), (**h**) calmodulin 6 (CL1694.Contig1_All), (**i**) NADH/NADPH oxidase (CL10431.Contig2_All), (**j**) mitogen-activated protein kinase 15 (CL314.Contig3_All), (**k**) mitogen-activated protein kinase kinase 2 (CL772.Contig1_All), and (**l**) mitogen-activated protein kinase kinase kinase 1. The different lowercase letters in the same tissue represent a significant difference at the 0.05 level.