## 5 Microarray Results
The IPA pathway analysis revealed a significant association between cardiac fibrosis with a set of 10 molecules (Airn, ARG1, ATF3, CCN2, CYP1B1, EGR1, HAND2, LCN2, PFKFB1, SCN5A) in the PHD1\({}^{-/-}\)MI group (\(p\) = 0.0000382). The complete information of networks clustered by IPA is provided in Table 3, which contains 13 networks. IPA was used to construct a graphical network of important networks 5 (Figure 6A), 11 (Figure 6B), 12 (Figure 6C) and 13 (Figure 6D). Table 4 lists related cardiac fibrosis genes and their role in fibrosis. The list of (anticipated) activated or inhibited regulators that can raise, or lower functional molecules downstream is displayed in Table 5. Ingenuity pathway analysis identified several anti-inflammatory diseases and several anti-inflammatory molecules, as listed in Table 6. In addition, Table S1 provides additional information related to the canonical pathway. Several metabolic and cell signaling pathways are listed in this table. Table S1 shows both key biological and molecular roles of proteins and chemicals of interest. Table 7 shows the results of gene enrichment analysis of top 30 pathways identified. \begin{table}
\begin{tabular}{l l l l l} \hline  & \multicolumn{2}{c}{**Gene Symbol**} & \multicolumn{1}{c}{**Description**} & \multicolumn{1}{c}{**Fold Change**} & \multicolumn{1}{c}{_p-Value_} \\ \hline
62 & Trim30d & tripartite motif-containing 30D & \(-1.56\) & 0.0012 \\ \hline
63 & Idh1 & isocitrate dehydrogenase 1 & \(-1.56\) & 0.003 \\ \hline
64 & Ednra & endothelin receptor type A & \(-1.56\) & 0.0243 \\ \hline
65 & Aldh3b3 & aldehyde dehydrogenase 3 family, member B3 & \(-1.56\) & 0.0281 \\ \hline
66 & Serpina3m & serine (or cysteine) peptidase inhibitor, clade A, member 3M & \(-1.55\) & 0.0025 \\ \hline
67 & Phka1 & phosphorylated kinase alpha 1 & \(-1.54\) & 0.001 \\ \hline
68 & Pfkfb1 & 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 & \(-1.54\) & 0.0062 \\ \hline
69 & Abhd6 & abhydrolase domain containing 6 & \(-1.53\) & 0.0043 \\ \hline
70 & Mfap5 & microfibrillar associated protein 5 & \(-1.53\) & 0.0163 \\ \hline
71 & Chp1 & calcineurin-like EF hand protein 1 & \(-1.52\) & \(5.06\times 10^{-6}\) \\ \hline
72 & Scn5a & sodium channel, voltage-gated, type V, alpha & \(-1.51\) & \(4.44\times 10^{-7}\) \\ \hline
73 & Mrap & melanocortin 2 receptor accessory protein & \(-1.51\) & 0.0034 \\ \hline \end{tabular}
\end{table} 
Table 2: _Cont._Figure 4: (**A**) Volcano plot and (**B**) scattered plot. Red dots represent positive fold change, while green dots represent negative fold change. (**C**) Heat map provides overall information of differentially regulated genes in a pictorial representation format of WTMI and PTID1\({}^{-}\)\({}^{-}\)MI comparisons. Heat map showing differentially expressed genes in WT and PTID1\({}^{-}\)\({}^{-}\)MI groups. The blue boxes indicate downregulated expression of genes, whereas the red boxes indicate upregulated expression of genes. The intensity of the red shade represents the degree of upregulation, while the degree of downregulation is the intensity of the blue shade. \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Network** & **Molecules in Network** & **Score** & **Focus Molecules** & **Top Diseases and Functions** \\ \hline  & ABRA, Akt, BDH1, Calineurin (complex), & & & \\ Calineurin & A, calpain, CaMKII, Cdk (family), & & & \\ CHPI, Cyclin A, Cyclin D, E2f, Fam13a, FcER1, & & & \\ Gsk3, HAND2, Igrp, MAC, MEF2, MFAP5, & & & \\ MTSS1, NADPH oxidase, Nedd4, NFAT & & & \\ (complex), Ngp, Osteocalcin, PDGF (complex), & & & \\ PFKFB1, PHKA1, PLA2, RASL11B, Rb, SCN4B, & & & \\ SCN5A, STAT3/STAT3 & & & \\ \hline  & ABAT, ADCY, ALDOB, Alp, AMY2A, & & \\ ANKRD9, APOD, ATF3, Calmodulin, CK2, & & & \\ Collagen Alpha1, Creb, EGLN, FOSB, GDPD3, & & & \\ GNRH, IgG, Insulin, IRS1, IRS2, Jnk, Kcnn2, & & & \\ LCN, LCN2, Myosin, Nos, NRA1, NRN1, Pde, & & \\ PKA, PRKD, Proinsulin, PTGDS, ROCK, Rsk & & \\ \hline \hline \end{tabular}
\end{table} 
Table 3: List of networks identified from ingenuity pathway analysis.