## 3 Results
### Genome Description and Taxonomic Classification of Geobacillus sp. G4  
The results regarding the preliminary genome draft of _Geobacillus_ sp. G4 is presented in Table 2. The strain genome has a size of approximately 3.4 Mb with a coverage of \(\sim\)1225x. The assembly comprised 164 contigs, with a total G+C content of 52.59%. The genome completeness and contamination were 99.45% and 0.95%, respectively. The N50 and L50 values for the assembly were 236,119 bp and 5 contigs, respectively. The annotation identified a total of 3490 genes: 2047 coding sequences (CDS), 5 rRNAs, 88 tRNAs, 1 transfer-messenger RNA, and 1349 putative proteins. Regarding the gene clusters related \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Phenotypic Characteristics** & _Geobacillus_**sp.** & _G. kaustophilus_ & _G. thermoleocorans_ \\  & **G4 * & **ATCC 8005 ** & **ATCC 43513 ** \\ \hline NaCl (\%, \(w/v\)) & 0–2.5 & 0–2 & 0–4 \\ Temperature range (\({}^{\circ}\)C) & 50–70 & 40–75 & 37–70 \\ Acetate & + & + & + \\ Citrate & - & + & - \\ \hline \hline \end{tabular}  
* Unpublished data; ** from [PERSON] and [PERSON] (2020) [1].  
\end{table} 
Table 1: _Cont._  
Figure 2: Quantitative and qualitative enzymatic activity of _Geobacillus_ sp. G4. Extracellular enzymatic activities of _Geobacillus_ sp. G4. **(A)** Formation of clear halos on agar plates indicating positive extracellular enzyme production: Amy (amylase), Pec (pectinase), Xyl (xylanase), Cel (cellulose), Prot (protease), and Lip ( lipase). **(B)** Enzyme production profiles measured in LB liquid media over time. to secondary metabolism (BCGs), seven clusters were identified: betalactone, T3 PKS, Lanthopeptide class-i, NI-siderophore, terpene, fengycin, and RiPP-like. The nucleotide average identity (ANI) values between the _Geobacillus_ sp. G4 genome and other species of the _Geobacillus_ genus show similarity with _Geobacillus kaustophilus_ and _Geobacillus thermoleovorans_, with ANI values above 97% (Table 3). Thus, the obtained results suggest that _Geobacillus thermoleovorans_ KCTC3570 and _Geobacillus_ sp. G4 are sufficiently similar to be considered as belonging to the same species. \begin{table}
\begin{tabular}{c c c c c} \hline
**General Data** & **Assembly** & **Reference Genome** & **Reference Genome** & **Reference Genome** \\
**De Novo** & **G. kaustophilus** & **G. thermoleovorans** & **G. kaustophilus** \\  & _Geobacillus_ **sp. G4** & **HTA426** & **KCTC 3570** & **NBRC102445** \\ \hline _Contigs_ & 164 & 2 & 2 & 1 \\ Total length & 3,362,411 & 3,544,776 & 3,450,609 & 3,670,957 \\ N50 (KB) & 236,119 & 3,716,831 & 1,192,538 & 750,728 \\ L50 & 5 & 1 & 2 & 1 \\ GC (\%) & 52.59 & 52.35 & 52.50 & 51.73 \\ Coverage & 1224.95 & - & 224.2 & 150.5 \\ Completeness (\%) & 99.45 & 99.31 & 99.44 & 93.81 \\ Contamination (\%) & 0.95 & 0.22 & 30.89 & 0.24 \\ CDS & 2047 & 5668 & 4714 & 5548 \\ rRNA & 5 & 37 & 32 & 35 \\ tRNA & 88 & 129 & 100 & 111 \\ tmRNA & 1 & 2 & 1 & 3 \\ Genes & 2047 & 3655 & 3622 & 3734 \\ Putative protein & 1349 & 3386 & 3369 & 3401 \\ \hline \end{tabular}
\end{table} 
Table 2: Genome assembly data for _Geobacillus_ sp. G4 was performed using SPAdes v.3.15.4. The annotation was conducted using Prokka v.1.14.6, and quality parameters were assessed using Busco v.5.5.0 and Quast v.5.2.0. All processes were carried out on the Galaxy v24.1.2 server.