## 3 Results and Discussion
### Gas Chromatography-Mass Spectrometry (GC-MS) Analysis
It was demonstrated that the rutin and tamarixetin contained in _Berberis_ were responsible for the inhibition of cancer cell lines in a concentration-or time-dependent manner by inducing apoptosis and blocked cell cycle progression at the G2-M phase [10]. Anti-proliferative activity in breast (MCF-7), colon (Caco-2), and pancreas (BxPC-3) cancer cell lines induced by Genus _berberis_ was well reported elsewhere (21). Moreover, rutin exhibited a dose-or time-dependent inhibitory effect on U-937 and HT-60 and glioma human cancer cell lines [12; 19; 20; 21; 22]. [PERSON] et al. (2020) [12] reported that the most abundant compounds found in _B. hispanica_ are phenolic acids, primarily chlorogenic acid and other caffeoylquinic acids. These compounds have been reported to have antioxidant effects [10; 23]. Moreover, these compounds have also been shown to possess an in vivo and in vitro anticancer activity in various cancerous cell lines including MCF-7, HCT-116, Hep-G2, and PC-3. Some compounds from caffeoylquinic acids were reported to have an inhibitory effect on the stomach (KatoIII), colon (DLD-1), and promyelocytic leukemia cancer cell lines (HL-60) [24; 25]. The results obtained in this work showed that _B. hispanica_ possesses interesting antioxidant and antiproliferative activities, which can be explained by its richness in phenolic, alkaloids, and other potentially bioactive compounds. This species appears to be an interesting source of various compounds, which can be applied in medicines to fight such diseases. \begin{table}
\begin{tabular}{c c c c c} \hline  & **RT** & **Molecular Weight** & **Formula** & **Compound Name** & **Area (\%)** \\ \hline
24 & 15.94 & 201.22 & C\({}_{12}\)H\({}_{11}\)NO\({}_{2}\) & trans-Ethylalpha cyanocinnamate & 0.313 \\ \hline
25 & 16.84 & 186.33 & C\({}_{12}\)H\({}_{26}\)O & 2-Dodecanol & 0.435 \\ \hline
26 & 16.91 & 212.42 & C\({}_{15}\)H\({}_{32}\) & Pentadecane & 1.075 \\ \hline
27 & 17.06 & 184.36 & C\({}_{13}\)H\({}_{28}\) & 2-Methyldodecane & 1.075 \\ \hline
28 & 18.04 & 256.42 & C\({}_{16}\)H\({}_{32}\)O\({}_{2}\) & n-Hexadecanoicacid & 1.993 \\ \hline
29 & 18.84 & 280.5 & C\({}_{20}\)H\({}_{40}\) & 9-Eicosene, (E) & 0.643 \\ \hline
30 & 19.23 & 212.41 & C\({}_{15}\)H\({}_{32}\) & Dodecane, 2,6,10-trimethyl & 1.325 \\ \hline
31 & 19.88 & 378.6 & C21H3404 Si & Decan-2-yl trimethylsilyl phthalate 1,2 & 0.702 \\  & & & & Benzenedicarboxylic acid & 0.702 \\ \hline
32 & 20.21 & 292.25 & C\({}_{16}\)H\({}_{11}\)F\({}_{3}\)O\({}_{2}\) & 1-fluoreencarboxylic acid, 2,2,2-trifluoroethyl ester & 1.662 \\ \hline
33 & 20.48 & 324.6 & C\({}_{23}\)H\({}_{48}\) & Heptadecane, 9-hexyl & 0.320 \\ \hline
34 & 21.17 & 322.5 & C21H38O2 & 11,14-Eicosadienoic acid, methyl ester & 14.287 \\ \hline
35 & 21.20 & 280.4 & C\({}_{18}\)H\({}_{32}\)O\({}_{2}\) & Linoelaidic acid & 14.287 \\ \hline
36 & 21.71 & 254.5 & C\({}_{18}\)H\({}_{38}\) & 2,6,10-trimethyl-pentadecane & 7.151 \\ \hline
37 & 21.98 & 212.42 & C\({}_{15}\)H\({}_{32}\) & Pentadecane & 7.151 \\ \hline
38 & 22.49 & 166.26 & C\({}_{11}\)H\({}_{18}\)O & 2,6-Nonadienal, 3,7-dimethyl & 15.390 \\ \hline
39 & 23.02 & 240.5 & C\({}_{17}\)H\({}_{36}\) & Tetradecane, 2,6,10-trimethyl & 3.445 \\ \hline
40 & 23.58 & 272.9 & C\({}_{17}\)H\({}_{33}\) & 7-Heptadecene & 15.390 \\ \hline
41 & 24.07 & 100.16 & C\({}_{6}\)H\({}_{12}\)O & 2-Hexen-1-ol, (E) & 0.514 \\ \hline
42 & 24.56 & 118.17 & C\({}_{6}\)H\({}_{14}\)O\({}_{2}\) & meso-3,4-Hexanediol & 0.529 \\ \hline
43 & 25.09 & 130.23 & C\({}_{8}\)H\({}_{18}\)O & 2-Hexanol, 3,4-dimethyl & 0.683 \\ \hline
44 & 25.64 & 112.17 & C\({}_{7}\)H\({}_{12}\)O & 2-Heptenal, (E) & 0.324 \\ \hline
45 & 26.15 & 170.33 & C\({}_{12}\)H\({}_{26}\) & Dodecane & 0.698 \\ \hline \end{tabular}
\end{table} 
Table 2: _Cont._