## 4 Discussions  
This study obtained an insight into the effect of CFS from _A. sobria_ on the spoilage of _S. putrefaciens_ in Pacific white shrimp in different cold chain logistics during transportation, retailing, and domestic storage, which have a negative effect on shrimp [18,19]. Our previous research also indicated that _Shewanella_ and _Aeromonas_ species were identified the specific spoilage bacteria in the spoiled Pacific white shrimp and that CFS from _A. sobria_ could inhibit the growth ability and the spoilage abilities of _S. putrefaciens_ during the refrigerated condition. Therefore, the effect of CFS from _A. sobria_ on the spoilage of _S. putrefaciens_ in Pacific white shrimp in different cold chain logistics is a worthy subject \begin{table}
\begin{tabular}{c c c c c c c c c c} \hline \hline \multirow{2}{*}{**Amino Acid**} & \multicolumn{3}{c}{**0 day**} & \multicolumn{3}{c}{**9 days**} \\ \cline{2-10}  & **CK** & **SA** & **CK1** & **CK2** & **CK3** & **SA1** & **SA2** & **SA3** \\ \hline Asp & 621.56 \(\pm\) 0.18 & 645.59 \(\pm\) 0.04 & 519.92 \(\pm\) 0.43 & 479.02 \(\pm\) 0.18 & 457.21 \(\pm\) 0.12 & 587.89 \(\pm\) 0.11 & 548.36 \(\pm\) 0.48 & 516.02 \(\pm\) 0.82 \\ Thr & 182.45 \(\pm\) 0.53 & 163.15 \(\pm\) 0.39 & 129.91 \(\pm\) 0.24 & 114.47 \(\pm\) 0.41 & 101.24 \(\pm\) 0.26 & 156.50 \(\pm\) 0.33 & 148.37 \(\pm\) 0.08 & 130.11 \(\pm\) 0.16 \\ Ser & 259.32 \(\pm\) 0.36 & 264.18 \(\pm\) 0.76 & 221.48 \(\pm\) 0.22 & 222.30 \(\pm\) 0.17 & 201.45 \(\pm\) 0.36 & 244.06 \(\pm\) 0.23 & 209.29 \(\pm\) 0.11 & 225.16 \(\pm\) 0.20 \\ Glus & 982.76 \(\pm\) 0.08 & 992.48 \(\pm\) 1.86 & 570.64 \(\pm\) 0.24 & 529.44 \(\pm\) 0.04 & 810.81 \(\pm\) 0.17 & 891.34 \(\pm\) 0.07 & 869.34 \(\pm\) 0.08 & 856.17 \(\pm\) 0.08 \\ Gly & 351.89 \(\pm\) 0.74 & 369.01 \(\pm\) 0.01 & 312.10 \(\pm\) 0.26 & 289.35 \(\pm\) 0.58 & 271.78 \(\pm\) 0.37 & 331.35 \(\pm\) 0.76 & 326.42 \(\pm\) 0.14 & 310.45 \(\pm\) 0.23 \\ Ala & 498.99 \(\pm\) 0.67 & 480.02 \(\pm\) 0.07 & 441.31 \(\pm\) 0.40 & 355.50 \(\pm\) 0.18 & 333.01 \(\pm\) 0.42 & 403.13 \(\pm\) 0.53 & 391.39 \(\pm\) 0.11 & 370.02 \(\pm\) 0.20 \\ Val & 129.18 \(\pm\) 0.82 & 141.99 \(\pm\) 0.74 & 153.05 \(\pm\) 0.45 & 1297.12 \(\pm\) 0.36 & 127.40 \(\pm\) 0.14 & 113.04 \(\pm\) 0.38 & 108.35 \(\pm\) 0.26 & 105.09 \(\pm\) 0.33 \\ Met & 181.03 \(\pm\) 0.31 & 167.14 \(\pm\) 0.17 & 149.45 \(\pm\) 0.57 & 135.30 \(\pm\) 0.48 & 132.37 \(\pm\) 0.23 & 131.91 \(\pm\) 0.72 & 129.49 \(\pm\) 0.27 & 126.34 \(\pm\) 0.82 \\ Ile & 94.34 \(\pm\) 0.33 & 95.01 \(\pm\) 0.36 & 92.40 \(\pm\) 0.25 & 69.32 \(\pm\) 0.20 & 79.01 \(\pm\) 0.26 & 89.91 \(\pm\) 0.51 & 788.81 \(\pm\) 0.41 & 80.02 \(\pm\) 0.09 \\ Leu & 403.61 \(\pm\) 0.24 & 51.48 \(\pm\) 0.40 & 323.82 \(\pm\) 0.16 & 300.45 \(\pm\) 0.45 & 278.30 \(\pm\) 0.51 & 3890.1 \(\pm\) 0.49 & 357.38 \(\pm\) 0.53 & 357.91 \(\pm\) 0.81 \\ Tyr & 221.91 \(\pm\) 0.14 & 239.07 \(\pm\) 0.32 & 170.51 \(\pm\) 0.14 & 167.01 \(\pm\) 0.39 & 140.42 \(\pm\) 0.18 & 189.32 \(\pm\) 0.32 & 179.04 \(\pm\) 0.30 & 161.34 \(\pm\) 0.41 \\ Phe & 231.94 \(\pm\) 0.14 & 241.06 \(\pm\) 0.23 & 170.32 \(\pm\) 0.29 & 169.69 \(\pm\) 0.10 & 140.492 \(\pm\) 0.14 & 188.92 \(\pm\) 0.32 & 177.74 \(\pm\) 0.44 & 165.80 \(\pm\) 0.88 \\ Lys & 460.73 \(\pm\) 0.76 & 453.74 \(\pm\) 0.29 & 395.28 \(\pm\) 0.41 & 367.51 \(\pm\) 0.26 & 338.91 \(\pm\) 0.76 & 401.49 \(\pm\) 0.49 & 394.20 \(\pm\) 0.14 & 378.91 \(\pm\) 0.72  & 37.91 \(\pm\) 0.72  \\ His & 1131.01 \(\pm\) 0.54 & 1201.09 \(\pm\) 0.48 & 110.09 \(\pm\) 0.23 & 90.91 \(\pm\) 0.48 & 80.81 \(\pm\) 0.81 & 103.45 \(\pm\) 0.07 & 94.35 \(\pm\) 0.85 & 870.81 \(\pm\) 0.81 \\ Arg & 505.51 \(\pm\) 0.49 & 490.30 \(\pm\) 0.14 & 47.18 \(\pm\) 0.32 & 412.19 \(\pm\) 0.14 & 580.30 \(\pm\) 0.27 & 40.622 \(\pm\) 0.14  & 434.35 \(\pm\) 0.36  & 419.45 \(\pm\) 0.48  \\ Total & 5264.23 & 4834.19 & 447.67.74 & 3985.1 & 3883.32 & 4684.62 & 4468.11 & 4262.56 \\ \hline \hline \end{tabular} Note: 1. Different letters (a–d) at each column denote significant differences (\(p<0.05\)) between means according to [PERSON]’s multiple range test.  
\end{table}