## 4 Discussion  
The formation of bacterial colonies in the middle of the media indicates that the bacteria that grow are anaerobic, or can grow in the absence of oxygen (Figure 1). The purple bacteria under the microscope indicate that they are Gram-positive bacteria, because the thick peptidoglycan on the bacterial cell wall absorbs the crystal violet color, allowing it to adhere to the cell wall and not to be dissolved by ethanol [11]. The cell diameters of the indigenous bacterial isolate ranged from 1.26 to 2.34 m.  
The cell morphology and bacterial colony indicates that the bacteria found have probiotic potential. The catalase test is the next characteristic. The catalase reaction produces positive results when air bubbles develop that indicate the presence of O\({}_{2}\), and negative results when no gas bubbles occur [16]. Because indigenous bacterial isolates are catalase negative, they can be studied further as potential probiotic microorganisms. Because H\({}_{2}\)O\({}_{2}\) compounds can be generated during aerobic metabolism, the catalase test can also be used to identify bacterial groupings based on oxygen demand because only bacteria that can grow in aerobic settings can breakdown these compounds. According to Figure 2, indigenous bacterial isolates have potential qualities as probiotic bacteria due to their strong development in acid and bile salt environments, allowing them to survive the digestive tract. The human digestive tract begins in the mouth with a pH condition determined by food intake, travels through the stomach at pH 2, then in the small intestine at pH 7-8, and finally in the colon at pH 5-8 [17]. LAB can be employed as a probiotic agent if it can live in the digestive tract; is resistant to heat, acids, and bile salts; and can correctly develop and colonize the intestines [16]. This enhances the case for indigenous bacteria as probiotic bacterium candidates. According to the findings in Figure 2b, indigenous bacterial isolates can survive at high temperatures. The third day of incubation at 45 \({}^{\circ}\)C in the exponential phase resulted in a 360.45% increase in OD. This supports the notion that isolated indigenous bacteria are potential probiotic bacteria. _Lactiplantiobacillus pentosus_, a probiotic bacteria, may grow optimally at 45 \({}^{\circ}\)C after 24 h of fermentation, yielding 20.53 mM gamma-aminobutyric acid [18]. As a result, additional screening or selection testing is required, such as sensitivity to low pH, gastric fluids, bile salts, pancreas, and preventing the growth of other harmful microorganisms. Next, the bacteria are evaluated for their capacity to grow at low pH levels, strengthening the prediction outcome. The purpose of this test is to confirm that the discovered bacteria are capable of surviving in an acidic environment. Figure 2c demonstrates the ability of native bacterial isolates to endure at pH 2, 3, and 4. The isolated bacterial isolates are possibilities for probiotic bacteria if they can survive at pH 2 for 100 min. Due to its  
Figure 7: Molecular weight of partial bacteriocin by SDS-PAGE electrophoresis analysis. Lane A, molecular markers, Lane D, position of peptide band bacteriocin from \(L\). _plantarum_-12 INH. capacity to keep an extracellular pH that is more alkaline than its cytoplasmic pH, LAB has a high level of tolerance. A significant proton gradient would not emerge in LAB since the intracellular pH changes dynamically along with the drop in external pH. When a large proton gradient forms, poor conditions result because it may lead to proton translocation, which consumes a lot of energy, as well as the buildup of anions and organic acids in the cytoplasm, which are toxic to these cells [19]. D-Cellobiose, sacacharose/sucrose, arbutin, esculin hydrolysis, phenylalanine arylamidase, D-glucose, 5-bromo-4-chloro-3-indoxyl-beta-glucose, D-mannose, and L-pyrrolidonyl arylamidase can all be assimilated by native bacterial strains. Native bacterial isolates had a 98% chance of _L. plantarum_ based on these biochemical traits.