# Objective Assessment of Porcine Voice Acoustics for Laryngeal Surgical Modeling
## 3 Results  
In total, 146 squeals from the three pigs did demonstrated variable features among the subjects. Table 3 summarizes the statistical analysis, with abbreviations of all parameters with statistically significant changes between before and after surgery. No parameters exhibited statistically significant change in all three pigs. Q50 and Flux changed in the two male pigs but did not reach statistical significance in the female. Q50\({}_{\text{min}}\) changed statistically significantly in the female and one male pig. Parameters changed most prominently in pig 3, which was also the only pig with highly significant changes (\(p\leq 0.001\)). Corrected \(p\)-values for all parameters and comparisons can be found in Table S3 in the supporting information.  
\begin{table}
\begin{tabular}{c c} \hline \hline
**Pig\#** & **Pre/Post \(p\leq 0.05\)** \\ \hline
1 (M) & Q50 *;SF *;Flux * \\
2 (F) & Q50\({}_{\text{min}}\) * \\
3 (M) & Q50 ***,Q50\({}_{\text{min}}\) * \\  & Q500 *** \\ \hline \hline \end{tabular}
\end{table}
Table 3: Statistically significantly changing parameters for all pre/post-surgery comparisons.  
Figure 3: Step-by-step illustration of the data analysis process.  
Table 4 shows mean values and standard deviations for F0-based voice measures. These parameters seem to vary randomly with no clear changes in any direction. Jit exhibits the most notable differences on average between pre- and post-treatment, but not uniformly for all pigs.  
In Figure 4, boxplots for all frequency-based parameters (from PF to Q25, see Table 2) are given for all three pigs (from (a) pig 1 to (c) pig 3). The \"box\" of each boxplot denotes the inter quartile range (from the first to the third quartile) with the horizontal line indicating the position of the median. The whiskers are calculated according to Matlab default settings as a multiple of the inter quartile range extending to the farthest data point within the potential whisker range. Everything above or below the whiskers is marked as an outlier.  
Statistically significant changes are marked with * symbols analogously to Table 3. In general, median values for all these parameters decreased after surgery However, those parameters based on eleven partial windows (Q502, Q5010 and Q500 min) changed less consistently. Overall, these findings reflect a condensed frequency range after surgery in all pigs. Pig 2 (the only female) exhibited the smallest initial frequency range and also the smallest change after surgery.  
Analogously, Figure 5 shows boxplots of the remaining parameters (Q50\({}_{\text{n}}\) to HNR). Values for SFQ50, Flux and RMSI were scaled by factors of 100, 300, and 10 to allow depiction of all these parameters in one plot. For the respective parameter units, refer to Table 2. Parameters in this figure change less consistently between pre- and post-surgery; only Flux displays statistically significant change, in more than one pig. Further, contrary to the stated H0 in Table S2, HNR increased for two pigs after surgery and minimally decreased in one.  
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Pig\#** & **Jit** & **Jit** & **Shim** & **Shim** & **CPP** & **CPP** \\
**[Mean]** & **[Std]** & **[Mean]** & **[Std]** & **[Mean]** & **[Std]** \\ \hline
1 pre & 0.083 & 0.013 & 0.205 & 0.017 & 14.218 & 1.396 \\
1 post & 0.091 & 0.011 & 0.206 & 0.018 & 15.181 & 2.000 \\
2 pre & 0.085 & 0.014 & 0.212 & 0.012 & 14.554 & 1.713 \\
2 post & 0.061 & 0.016 & 0.206 & 0.018 & 14.319 & 1.751 \\
3 pre & 0.078 & 0.022 & 0.191 & 0.026 & 15.980 & 2.682 \\
3 post & 0.096 & 0.015 & 0.219 & 0.026 & 13.296 & 1.291 \\ \hline \hline \end{tabular}
\end{table}
Table 4: Mean and standard deviation (std) of F0-based parameters for all pigs pre- and post-surgery.  
Figure 4: Boxplots of frequency-based parameters for (**a**) pig 1, (**b**) pig 2, and (**c**) pig 3 for pre- and post-surgery recordings. Statistically significant changes are marked with * symbols. (* \(p\leq 0.05\), *** \(p\leq 0.001\)).