## 4 Discussion
The first is the acidification of the rhizosphere and the reduction of Fe\({}^{3+}\) ions by membrane-bound Fe\({}^{3-}\) chelate reductase, after which the root cells absorb Fe\({}^{2+}\), while the second approach in the acquisition of iron is the secretion of low molecule plant siderophore in order to solubilize the bound iron, after which the membrane proteins transport it into the root cells [52; 53]. [PERSON] et al. [54] described some siderophore-producing microorganisms obtained from the rhizosphere, which enhance plant growth in wheat cultivation and at the same time inhibit plant pathogen _Fusarium solani_. Siderophoresact as virulence factors in several pathogenic microorganisms, and it has been proven that siderophores of mutualistic and commensal species can help minimize the increase in plant pathogens [55]. The siderophore genes observed in our study are of the aliases EC 1.3.1.28, which are involved in the production of 2,3-dihydroxybenzoate, an important precursor of the siderophores bacilliibactin and enterobactin [56; 57]. Of note are the siderophore receptors gene like _lutA_, which also play a key role in virulence in pathogens, as mutation in siderophore receptors alters the transport of siderophore-iron complex [58]. In addition, the metagenomes revealed genes Ferric hydroxamate ABC transporter (TC 3.A.1.14.3), _fluU_, _fluU_, and _fluB_, which help in ATP-binding protein, periplasmic substrate-binding protein, and permease component, all coding for Ferric hydroxamate ABC transporter. In a study by [PERSON] et al. [59] hydroxamate siderophore produced by _Streptomyces tendae_ F4 strain was proven to enhance cadmium and iron uptake in sunflower plants, consequently promoting the growth of the plant. Phosphorus is a key component in plant nutrition, playing an important function in metabolic processes such as photosynthesis, nutrient transport, cell division, and signal transduction. Most organic and inorganic phosphates in the soil are in an insoluble form, and a sizeable number of them cannot be used by plants [60]. Phosphate solubilizing genes were well represented in our study. _pqqABCDE_ gene, a protein coenzyme that is involved in the biosynthesis of pyrroloquinoline quinone (PQQ), plays an important function in solubilizing phosphates in the soil, thereby making it useful for plants [61]. In a study conducted by [PERSON] et al. [62], bacterial isolate _Burkholderia cenccepacia_ CR318 isolated from the rhizosphere of maize was confirmed to have a mechanism that can solubilize inorganic phosphate and subsequently improved the growth of the maize plant. Phosphate-solubilizing microorganisms have been well established in the rhizosphere, where they possess the ability of solubilizing soil insoluble phosphate by releasing organic acid, most especially gluconic acid (GA), 2-ketogluconic acid, tartaric acid, oxalic acid, citric acid, and other carboxylic acids [63]. The organic acid chelates divalent cations from complex phosphorus mineral-like tricalcium phosphate and hydroxyapatite and releases free P, which the plant eventually takes up [64]. Findings have shown the important role that PQQ synthesis-using phosphate solubilizing microorganisms play, thus establishing microbial phosphate solubilization as one of the important requirements for plant growth [65]. Serine proteases (EC 3.4.21.) are enzymes cleaving peptide bonds normally found in proteins. They are ubiquitous in nature, present in both prokaryotes and eukaryotes, with important biological functions [66]. Proteases that are found in soil originate from different sources including plants, microorganisms, and animals. For instance, the proteases from plant biomass or microbial processes have been discovered to be relevant in the way a plant responds to environmental circumstances across several ecosystems [67]. We discovered two types of high-temperature stress response genes _degP/htrA_ in our study.