## 4 Discussion  
Until now, many models of sepsis have been described in animal experimentation, but most of them failed to replicate the human heterogeneous septic process, which is dependent on the genetic susceptibility of each individual and influenced by sex, age, comorbidities and drug consumption [18; 19]. The murine model described here generates an efficient, controlled and easily reproducible intraperitoneal infection that could serve as a basis for future lines of research. Scientific research based on the use of garlic derivatives has led to ambiguous conclusions on the beneficial effects of this plant, thus preventing the application of garlic products in the treatment of certain diseases [20]. This situation can be attributed to several factors, among which the following can be highlighted: the chemical instability of this type of compound [21], the great diversity of industrial processes for their production, the lack of coherence in terms of the medical properties investigated, and the chemical composition of the products used in these clinical investigations [22]. With the freeze-dried \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Variable, n/nt (\%)** & **CEF** & **CEF + TASE** & _p-Value_ \\ \hline Liver—bepatic congestion & 8/9 (89) & 8/9 (89) & - \\ Liver—sinusoidal PMN leukocytes & 4/9 (44) & 2/9 (22) & 0.62 \\ Liver—serosa PMN leukocytes & 3/9 (33) & 1/9 (11) & 0.576 \\ Liver—bacteria & 2/9 (22) & 0/9 (0) & 0.471 \\ Liver—perinuclear vacuolization & 6/9 (33) & 7/9 (78) & 0.599 \\ Peritoneum—PMN leukocytes & 3/9 (33) & 2/9 (22) & 0.999 \\ Peritoneum—bacteria & 3/9 (33) & 1/9 (11) & 0.576 \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Histopathological analysis and organs evaluation in relation to inflammation, bacteria, congestion, and vacuolization. \begin{table}
\begin{tabular}{c c c c c} \hline \hline  & **RESULTS OF MICRBIOLOGIC STUDIES** & & & \\ \hline \multirow{4}{*}{**Number of Rats**} & \multirow{2}{*}{**Blood Cultures**} & \multirow{2}{*}{**Peritoneal Liquid**} & **Antibiogram** \\  & & & **Culture** & **(Sensitive to Ampicillin,** \\  & & & 25922 & \\ \cline{1-1} \cline{3-5} \multirow{2}{*}{Control (Group I)} & \multirow{2}{*}{3} & Positive _E. coli_ ATCC & Positive _E. coli_ ATCC & \\  & & & 25922 & \\ \hline \multirow{4}{*}{\
\begin{tabular}{c} Ceftriaxone \\ (Group II) \\ \end{tabular} } & \multirow{2}{*}{8} & \multirow{2}{*}{6} & \multirow{2}{*}{_Enterococcus faecalis_} & 2–\text{\text{\textminus}} & \\ \cline{3-5}  & & & 4–Negative & \\ \cline{3-5}  & & & 25922 & \\ \cline{3-5}  & & & & Exitus before 24 h (no & - \\  & & & samples collected) & - & - \\ \hline \multirow{4}{*}{
\begin{tabular}{c} Ceftriaxone + \\ TASE * \\ (Group III) \\ \end{tabular} } & \multirow{2}{*}{8} & \multirow{2}{*}{_E. faecalis_} & 1–\text{\text{\textminus}} _E. faecalis_ & \\ \cline{3-5}  & & & 7–Negative & Sensitive \\ \cline{1-1} \cline{3-5}  & & 1 & Negative & Negative & - \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Results of blood and peritoneal fluid cultures stratified by treatment groups and their respective antibiogram. garlic used in this experimental model, these deficiencies could be overcome by using stable and known concentrations of allicin and other thiosulfinates over time. At present, there are no studies in the scientific literature that have demonstrated a decrease in morbidity and mortality in relation to sepsis, except for antibiotics and the goal-guided resuscitation strategy [23]. Some of the drugs that have been tested are, among others, corticoids [12], immunoglobulins [13], antithrombin III [14], vasopressin [24] or anti-TNF [25]. However, the clinical results obtained in our animal experimentation study are encouraging in this sense and confirm our working hypothesis showing earlier recovery of weight, less ocular secretions, separation of whiskers and decrease in hypoactivity in the group where TASE was administered.