# Bioremediation of Persistent Organic Pollutant--Oxybenzene with _Pleurotus diamor_
## 3 Results
### Identification of Biodegradation Products of Oxybenzene and Toxicity Evaluation
\begin{table}
\begin{tabular}{c c c c} \hline \hline
**Retention** & **Symbol in** & **Summary Formula** & \\
**Time (t\({}_{\text{r}}\)) (min)** & **Figure** & **[M+H\({}^{+}\)]** & **Daughter Ions [_m/z_]; Formula** \\ \hline \multirow{2}{*}{3.40} & \multirow{2}{*}{E} & \multirow{2}{*}{C\({}_{13}\)H\({}_{11}\)O\({}_{3}\)} & 137.0235; C\({}_{7}\)H\({}_{5}\)O\({}_{3}\) \\  & & & 105.0342; C\({}_{7}\)H\({}_{5}\)O\({}\) \\ \hline \multirow{2}{*}{3.50} & \multirow{2}{*}{F} & \multirow{2}{*}{C\({}_{15}\)H\({}_{15}\)O\({}_{2}\)} & 91.0548; C\({}_{7}\)H\({}_{7}\) \\ \hline \multirow{2}{*}{3.56} & \multirow{2}{*}{G} & \multirow{2}{*}{C\({}_{16}\)H\({}_{14}\)NO\({}_{4}\)S} & 274.0538; C\({}_{14}\)H\({}_{12}\)NO\({}_{3}\)S \\  & & & 259.0305; C\({}_{13}\)H\({}_{9}\)NO\({}_{3}\)S \\  & & & 237.0094; C\({}_{10}\)H\({}_{7}\)NO\({}_{4}\)S \\  & & & 105.0336; C\({}_{7}\)H\({}_{5}\)O \\ \hline \multirow{2}{*}{4.02} & \multirow{2}{*}{H} & \multirow{2}{*}{C\({}_{14}\)H\({}_{13}\)O\({}_{3}\)} & 151.0400; C\({}_{8}\)H\({}_{7}\)O\({}_{3}\) \\  & & & 105.0345; C\({}_{7}\)H\({}_{5}\)O \\ \hline \multirow{2}{*}{4.48} & \multirow{2}{*}{I} & \multirow{2}{*}{C\({}_{15}\)H\({}_{12}\)NO\({}_{3}\)S} & 208.0072; C\({}_{9}\)H\({}_{6}\)NO\({}_{3}\)S \\  & & & 180.0120; C\({}_{8}\)H\({}_{6}\)NO\({}_{2}\)S \\  & & & 152.0164; C\({}_{7}\)H\({}_{6}\)NOS \\ \hline \multirow{2}{*}{4.78} & \multirow{2}{*}{J} & \multirow{2}{*}{C\({}_{15}\)H\({}_{15}\)O\({}_{2}\)} & 91.0548; C\({}_{7}\)H\({}_{7}\) \\  & & & 165.0551; C\({}_{9}\)H\({}_{9}\)O\({}_{3}\) \\  & & & 105.0345; C\({}_{7}\)H\({}_{5}\)O \\ \hline \multirow{2}{*}{6.11} & \multirow{2}{*}{Oxygenzone} & \multirow{2}{*}{C\({}_{14}\)H\({}_{13}\)O\({}_{3}\)} & 151.0400; C\({}_{8}\)H\({}_{7}\)O\({}_{3}\) \\  & & & 105.0345; C\({}_{7}\)H\({}_{5}\)O \\ \hline \multirow{2}{*}{6.46} & \multirow{2}{*}{L} & \multirow{2}{*}{C\({}_{15}\)H\({}_{15}\)O\({}_{2}\)} & 151.0399; C\({}_{8}\)H\({}_{7}\)O\({}_{3}\) \\  & & & 91.0548; C\({}_{7}\)H\({}_{7}\) \\ \hline \multirow{2}{*}{7.08} & \multirow{2}{*}{M} & \multirow{2}{*}{C\({}_{15}\)H\({}_{15}\)O\({}_{2}\)} & 151.0399; C\({}_{8}\)H\({}_{7}\)O\({}_{3}\) \\  & & & 91.0548; C\({}_{7}\)H\({}_{7}\) \\ \hline \hline \end{tabular}
\end{table} 
Table 3: _Cont._ \begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Compound** & **Mutagenic** & **Tumorigenic** & **Irritant** & \begin{tabular}{c} **Reproductive** \\ **Effects** \\ \end{tabular} \\ \hline Product F & – & – & medium-risk fragment & – \\ \hline Product G & – & – & medium-risk fragment & – \\ Product H & – & – & – & – \\ \hline Product I & \begin{tabular}{c} medium-risk fragment \\ \end{tabular} & – & – & – \\ \hline Product K & – & – & – & – \\ \hline Product L & \begin{tabular}{c} high-risk fragment \\ \end{tabular} & – & \
\begin{tabular}{c} medium-risk fragment \\ \end{tabular} &
\begin{tabular}{c} high-risk fragment \\ \end{tabular} \\ \hline Product M & – & – & – & – \\ \hline \hline \end{tabular}
\end{table} 
Table 4: _Cont._  
Figure 4: Identified biodegradation products formed in the mycelium and the medium obtained from in vitro cultures of _P. djamor_ grown with the addition of oxygenzone. The predicted ecotoxicity of biodegradation products generated during incubation of _P. djamor_ mycelium with oxygenzone was unexpectedly high (Figure 5). For instance, the compound labeled \"L\" exhibited an ecotoxicity more than five times greater than that of oxybenzene. The predicted ecotoxicity of products I and J was higher than that of oxybenzene, while products C, D G, H and K had ecotoxicity levels lower than those of the initial compound.