## 3 Results and Discussion
### Chemical Constituents in the Lipid Fraction
\begin{table}
\begin{tabular}{c c c c c} \hline
**Peak No.** & **R\({}_{\text{t}}\) (min)** & **Rel. \%** & **Chemical Formula** & **Compounds** \\ \hline
1 & 7.74 & 3.85 & C\({}_{13}\)H\({}_{28}\) & Tridecane \\
2 & 11.35 & 1.98 & C\({}_{16}\)H\({}_{34}\) & Hexadecane \\
3 & 12.74 & 3.07 & C\({}_{17}\)H\({}_{36}\) & Heptadecane \\
4 & 14.22 & 8.08 & C\({}_{18}\)H\({}_{38}\) & Octadecane \\
5 & 15.65 & 10.01 & C\({}_{19}\)H\({}_{40}\) & Nonadecane \\
6 & 16.55 & 8.25 & C\({}_{20}\)H\({}_{42}\) & Eicosane \\
7 & 18.33 & 8.26 & C\({}_{21}\)H\({}_{44}\) & Heneicosane \\
8 & 19.48 & 23.54 & C\({}_{22}\)H\({}_{46}\) & Docosane \\
9 & 21.021 & 5.97 & C\({}_{23}\)H\({}_{48}\) & Tricosane \\
10 & 22.319 & 2.55 & C\({}_{24}\)H\({}_{50}\) & Tetracosane \\
11 & 22.964 & 4.04 & C\({}_{25}\)H\({}_{52}\) & Pentacosane \\
12 & 24.680 & 2.24 & C\({}_{26}\)H\({}_{54}\) & Hexacosane \\
13 & 25.258 & 5.92 & C\({}_{27}\)H\({}_{56}\) & Heptacosane \\
14 & 26.433 & 2.71 & C\({}_{28}\)H\({}_{58}\) & Octacosane \\
15 & 26.965 & 1,84 & C\({}_{29}\)H\({}_{60}\) & Nonacosane \\
16 & 27.674 & 1.09 & C\({}_{30}\)H\({}_{62}\) & Triacontane \\
17 & 30.369 & 0.96 & C\({}_{27}\)H\({}_{46}\)O & Cholesterol \\
18 & 31.132 & 3.40 & C\({}_{29}\)H\({}_{48}\)O & Stigmasterol \\
19 & 32.468 & 1.54 & C\({}_{29}\)H\({}_{50}\)O & _\(\beta\)_-Sitosterol \\
20 & 35.062 & 1.05 & C\({}_{30}\)H\({}_{50}\)O & _\(\alpha\)_-Amyrine \\ \hline \end{tabular}
\end{table} 
Table 2: GLC data of USF of _Lavandula pubescens_ Decne (LPD). The FTIR spectra of the adsorbent (LPD) and their alcoholic extract are shown in Figure 1. As can be seen, the majority of the functional groups can be identified, allowing the estimation of the adsorption active sites of the biosorbent under investigation. Both spectra exhibited prominent peaks at 3300, 2910, and 1645 cm\({}^{-1}\), while peaks at 1400, 1300, 1100, and 1041 cm\({}^{-1}\) were clearer in the alcoholic extract. However, the reduced intensity of some peaks in the spectra of the LPD, compared to the extract, may be due to the bulky materials, compared to the less complicated contents in the extract. The broad absorption band at 3300 cm\({}^{-1}\) was assigned to stretching vibrations of hydroxyl functionality in the alcoholic and carboxylic OH groups, while the stretching vibration of (NH) C=O peptide carbonyl groups appeared at 1645 cm\({}^{-1}\), with shoulders around 1750 cm\({}^{-1}\) indicating additional different type of carbonyl groups. Peaks corresponding to the aromatic C=C double bonds could be assigned at 1510 cm\({}^{-1}\). The band at 1400 cm\({}^{-1}\) was due to C-C and C-N stretching, and the peak at 2910 cm\({}^{-1}\) was assigned to C-H (e.g., methoxy compounds) stretching vibrations. The peak at 1041 cm\({}^{-1}\) corresponds to C-O stretching vibration of organic compounds, such as ethers, esters, and amides. Accordingly, in addition to the hydrocarbons, the GC/MS and GLC analysis indicate the presence of adequate amounts of hydroxyl-, carboxyl-, and amide-containing organic compounds such as  
Figure 1: Fourier transform infrared spectra of the adsorbent LPD (green-colored spectra) and the ethanol extract (blue-colored spectra). \begin{table}
\begin{tabular}{c c c c c} \hline
**Peak No.** & **Rt (min)** & **Rel. \%** & **Notation** & **Compounds** \\ \hline
1 & 21.15 & 23.69 & C16(0) & Palmitic acid \\
2 & 25.06 & 14.51 & C18(1) & Oleic acid \\
3 & 26.03 & 8.99 & C18(2) & Linoleic acid \\
4 & 27.04 & 13.72 & C18(3) & Linolenic acid \\
5 & 28.65 & 11.24 & C20(0) & Arachidic acid \\
6 & 29.48 & 3.30 & C20(4) & Arachidonic acid \\
7 & 30.75 & 3.24 & C22(0) & Behenic acid \\
8 & 32.45 & 21.31 & C24(0) & Lignoeceric acid \\ \hline \end{tabular}
\end{table} 
Table 3: Gas liquid chromatography (GLC) data of fatty acid methyl esters (FAME) fraction of _L. pubescens_ Decne. sterol, cholesterol, and long-chain saturated and unsaturated fatty acids.