## 3 Results
### Qualitative Anthocyanin Content by LC-MS-IT-TOF Analyses  
The results of the LC-MS-IT-TOF analyses clearly show that the ethanolic extracts contain more anthocyanins than acetone extracts. 25 anthocyanins were detected in the ethanol extract and 19 in the acetone extract. The LC-MS-IT-TOF profiles showed the advantage of using ethanol as the solvent for extraction from berry fruits.  
In our study, cyanide-3-glutoside is a common compound in the content of all studied samples. We also detected cyanide-3-galactoside, malvidine-3-galactoside, malvidine-3-glucose, cyanide-3,5-diglucoside and cyanide-3-rutinoside in various amounts, respectively. Cyanide-3,5-diglucoside, cyanide-3-rutinoside and, most importantly, cyanide-3-sambusioside were only found in _Sambucus nigra_ samples. In addition, malvidine-3-galactoside and malvidin-3-glucose were detected only in _Vaccinium_ species. The anthocyanin contents of lyophilizes after their ethanolic and acetone extraction and identification by LC-MS-IT-TOF are given in Tables 1-4.  
\begin{table}
\begin{tabular}{p{42.7 pt} p{42.7 pt} p{42.7 pt} p{42.7 pt} p{42.7 pt} p{42.7 pt} p{42.7 pt} p{42.7 pt} p{42.7 pt}} \hline \hline
**Compound** **Assigned** & **Rt (min)** & **Molecular Ions** & **Fragment Ions** & **Bilberry Vaccitium myrtillus** & **Highbush Diseberry Vaccitium myrtillus** & **Eldelberry Vaccitium myrtillus** & **Black Dabucus Aronnia** & **Chokeberry Aronnia melanocarpa** \\ \hline cyanidine-3,5-3,5-diglucoside & 8,985 & 611,616 & 449,112; 287,06 & - & - & \(\bullet\) & - \\ \hline cyanidine-3-sambubioside-5-glukoside & 9,390 & 743,202 & 581,158; 287,056 & - & - & \(\bullet\) & - \\ \hline delfinidine + hexose & 9,545 & 465,089 & 303,042 & - & \(\bullet\) & - & - \\ \hline delfinidine-3-galactoside & 9,565 & 465,102 & 303,051 & \(\bullet\) & - & - & - \\ \hline delfinidinidin-3-glucose & 10,005 & 465,102 & 303,051 & \(\bullet\) & - & - & - \\ \hline cyanidine-3-galactoside & 10,300 & 449,106 & 287,055 & \(\bullet\) & \(\bullet\) & - & - & \(\bullet\) \\ \hline delfinidine-3-raphoside & 10,375 & 435,093 & 303,050 & \(\bullet\) & \(\bullet\) & - & - & - \\ \hline cyanidine-3-glucoside & 10,677 & 449,106 & 287,055 & \(\bullet\) & \(\bullet\) & \(\bullet\) & \(\bullet\) & \(\bullet\) \\ \hline cyanidine-3-sambubiozid & 10,750 & 581,148 & 287,053 & - & - & \(\bullet\) & - \\ \hline gelatinidae-3-galakoside & 10,820 & 479,117 & 317,066 & \(\bullet\) & - & - & - \\ \hline petinidine + hexoze & 10,800 & 479,104 & 317,056 & - & \(\bullet\) & - & - \\ \hline cyanidine-3-raphoside & 10,970 & 419,098 & 287,055 & \(\bullet\) & \(\bullet\) & - & \(\bullet\) \\ \hline cyanidine-3-rutinoside & 11,072 & 595,164 & 449,108; 287,050 & - & - & \(\bullet\) & - \\ \hline petinidine-3-glucose & 11,105 & 479,118 & 317,066 & \(\bullet\) & - & - & - \\ \hline pelargonidine-3-galactoside & 11,260 & 433,113 & 271,060 & - & - & \(\bullet\) & - \\ \hline peonidine-3-galactoside & 11,360 & 463,120 & 301,070 & \(\bullet\) & - & - & - \\ \hline peonidine + hexoze & 11,365 & 463,110 & 301,060 & - & \(\bullet\) & - & - \\ \hline petinidine-3-raphoside & 11,420 & 449,108 & 317,067 & \(\bullet\) & \(\bullet\) & - & - \\ \hline pelargonidine-3-sambubio-side & 11,485 & 565,133 & 271,046 & - & - & \(\bullet\) & - \\ \hline peonidine-3-glucose & 11,690 & 463,124 & 301,070 & \(\bullet\) & - & - & - \\ \hline \hline \end{tabular}
\end{table}
Table 1: Anthocyanin contents of elderberry lyophilizes in regard to ethanolic fruit extract (\(\bullet\): presence; -: absence).  
\begin{table}
\begin{tabular}{p{42.7 pt} p{42.7 pt} p{42.7 pt} p{42.7 pt} p{42.7 pt} p{42.7 pt} p{42.7 pt} p{42.7 pt} p{42.7 pt}} \hline \hline