## 3 Results and Discussion
### Total Lipid, Cholesterol and Fatty Acid Composition  
The replacement of soybean with pea beans in the diet did not affect (\(p>0.05\)) lipid and cholesterol contents in chicken breast muscles (Table 4). Likewise, [PERSON] et al. [23] did not find any significant effect of diet with an increasing inclusion of field pea on total lipid content in the breast and drumstick muscles of Ross 308 broiler chickens. A similar result was reported by [PERSON] et al. [24] with fava bean used as a substitute of soybean meal in the diet. \begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline  & \multicolumn{2}{c}{**Diet (D) a**} & \multicolumn{2}{c}{**Genotype (G) b**} & \multicolumn{4}{c}{**Significance**} \\ \cline{2-9}  & **SOY** & **PEA** & **KB** & **NR** & **D** & **G** & **D \(\times\) G** \\ \hline Cholesterol, mg/100 g & 39.25 & 42.99 & 42.47 & 39.96 & 1.30 & ns & ns & ns \\ Lipids, \% & 1.16 & 1.16 & 1.21 & 1.12 & 0.04 & ns & ns & ns \\ Fatty Acids b, \% of total fatty acids & & & & & & & \\ C12:0 & 0.06 & 0.08 & 0.08 & 0.06 & 0.01 & ns & ns & ns \\ C14:0 & 0.42 & 0.49 & 0.45 & 0.46 & 0.02 & ns & ns & ns \\ C16:0 & 26.50 & 27.87 & 27.06 & 27.31 & 0.36 & ns & ns & ns \\ C16:1 & 1.45 & 1.98 & 1.54 & 1.89 & 0.11 & * & ns & ns \\ C17:0 & 0.21 & 0.20 & 0.21 & 0.20 & 0.01 & ns & ns & ns \\ C17:1 & 0.09 & 0.09 & 0.09 & 0.09 & 0.01 & ns & ns & ns \\ C18:0 & 11.57 & 11.34 & 11.65 & 11.26 & 0.19 & ns & ns & ns \\ C18:1n9 & 25.41 & 26.95 & 26.54 & 25.82 & 0.41 & ns & ns & ns \\ C18:2 n-6 & 24.80 & 22.74 & 23.96 & 23.59 & 0.27 & ** & ns & ns \\ C18:3 n-6 & 0.11 & 0.10 & 0.11 & 0.09 & 0.01 & ns & ns & ns \\ C18:3 n-3 & 1.00 & 0.78 & 0.97 & 0.81 & 0.03 & ** & * & ns \\ C20:2n6 & 0.39 & 0.33 & 0.40 & 0.33 & 0.01 & * & ** & ns \\ C20:3n6 & 0.40 & 0.42 & 0.41 & 0.41 & 0.02 & ns & ns & ns \\ C20:4 n-6 & 5.41 & 4.78 & 4.69 & 5.50 & 0.26 & ns & ns & ns \\ C20:5 n-3 & 0.08 & 0.06 & 0.06 & 0.08 & 0.01 & ns & ns & ns \\ C22:4n-6 & 1.15 & 1.03 & 1.02 & 1.16 & 0.06 & ns & ns & ns \\ C22:5n3 & 0.58 & 0.44 & 0.46 & 0.56 & 0.04 & ns & ns & ns \\ C22:6 n-3 & 0.37 & 0.31 & 0.31 & 0.37 & 0.03 & ns & ns & ns \\ \(\Sigma\)SFA & 38.76 & 39.97 & 39.44 & 39.29 & 0.34 & ns & ns & ns \\ \(\Sigma\)MUFA & 26.95 & 29.02 & 28.17 & 27.81 & 0.46 & * & ns & ns \\ \(\Sigma\)PUFA & 34.29 & 31.00 & 32.39 & 32.90 & 0.54 & ** & ns & ns \\ \(\Sigma\)n-6 & 32.26 & 29.40 & 30.58 & 31.08 & 0.49 & ** & ns & ns \\ \(\Sigma\)n-3 & 2.03 & 1.60 & 1.80 & 1.82 & 0.06 & ** & ns & ns \\ Nutritional indices c & & & & & & & & \\ n-6/n-3 & 16.43 & 18.92 & 17.48 & 17.87 & 0.47 & * & ns & ns \\ P/S & 0.89 & 0.78 & 0.83 & 0.84 & 0.02 & ** & ns & ns \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Effect of genotype and diet on cholesterol and total lipid content, fatty acid composition and nutritional indices in the breast muscle of broiler chickens. Genotype did not affect lipid and cholesterol contents in breast muscle (\(p>0.05\)). [PERSON] et al. (2013) observed a higher amount of lipids in fast- (Ross 308) than in medium- (Hubbard JA757) and slow-growing (ISA Dual) chickens; however, the differences between Hubbard JA757 and ISA Dual genotypes were low. On the other hand, no difference was observed for the cholesterol content between the genotypes studied by [PERSON] et al. (2013). Cholesterol content has become an important component in composition studies on meat and poultry products determining one of the attributes of the perception of quality. The cholesterol values obtained in the present study are quite similar to those reported in Ross 308 broiler chickens by [PERSON] et al. (2018) and lower (approximately by 13.7%) compared with those observed by [PERSON] et al. (2014). These differences between our study and cited works could be caused by the different genotypes of chickens as well as the rearing conditions and the method of feed preparation, including different seeds. It is known that the muscle FA profile reflects both endogenous biosynthesis and diet composition.