## 4 Discussion
In our study, plant pathogens presented less cold-adapted and more thermo-adapted proteins, indicating the potential to perform better in higher temperatures, which reinforces the findings of [137], showing that phytopathogens from the _Nectriaceae_ order are organisms that may prevail in global warming, increasing their distribution and impact. On the other hand, in some cases, the temperature can be a comprehensive factor that impacts cellular metabolism and functions. This encompasses essential biochemical parameters, such as the rates at which reactions occur, molecule binding, and the flexibility of cell membranes [132; 142]. As an example, freshwater saprobe fungal communities are susceptible to the effects of climate change in terms of their species composition and abundance; this, therefore, impacts freshwater ecosystems' functions [143; 144; 145; 146]. They are richer in cold regions or environments and have genome machinery that is more adapted to colder temperatures [54; 147]. Therefore, the increase in the temperature due to global warming could cause a reduction in their activity and could negatively impact energy, carbon, and nutrient cycling, threatening the delivery of ecosystem services to higher organisms [148; 149; 150; 151]. The newly assembled genome of _Neonectria lugdunensis_, presented in this study, was first isolated from a submerged decaying twig in a stream bed (personal communication, L. Marvanova) and is commonly reported in aquatic ecology studies [152] but has also been identified in soil [153]. _N. lugdunensis_ has been shown to be resistant to long droughs at 25 \({}^{\circ}\)C and has the potential to endure even higher temperatures for a limited time, as shown in [151]. Other freshwater fungi have better adaptation to colder temperatures, showing growth peaks at temperatures between 15 \({}^{\circ}\)C and 25 \({}^{\circ}\)C [144; 151]. The small amount of thermo-adapted proteins observed in the saprobe fungi in this study is concerning as the increase in the worldwide temperature could also lead to a decline in saprobe biodiversity, growth, and survival. The temperature is one of the most important factors influencing the structure of saprobe communities, and this may have severe consequences in the ecological process of decomposition [154]. Therefore, the monitoring of their biodiversity and the implementation of conservation efforts are vital to preserve the biodiversity of saprobe fungi. Protein synthesis is a central cellular process that is partly regulated by the availability of tRNA molecules. tRNAs can pre-present in the genome and spread in multiple families in multiple copies or single genes [155]. They are vital for the breakdown of organic matter and the synthesis of proteins and metabolites that can interact with living organisms. In endophytes and plant pathogens, they are essential for the organism's virulence and the evasion of host defense and are involved in pathogenicity [156; 157; 158]. Therefore, the number of copies of tRNA and their variability may be utilized to predict the efficiency with which genes may be translated. This allows for the estimation of protein synthesis rates, cells' responsiveness to external influences, and, eventually, evolutionary adaptations to novel environments, and it has been considered a determinant of lifestyle transitions among basidiomycetes [155; 159]. In our data, tRNA was found to be significant in different fungal lifestyles. The plant-pathogenic fungi in this study presented more tRNAs on average than saprobes and endophytes, in contrast to what was found in other studies [80; 81]. Fungi have developed distinct mechanisms for the production of CAZymes and secondary metabolites to suit their lifestyles [160; 161; 162; 163]. Secondary metabolites are primarily encoded by biosynthetic gene clusters (BGCs), which are jointly controlled and located very close together in a certain genome region; as a result, the BGCs responsible for a given specialized metabolite are either \"silent\" or upregulated [164; 165; 51; 166].