## 5 Conclusions  
The results of this study indicate that both deficit irrigation and the presence of _Ailanthus_ can influence the quality of olive oil from the 'Koroneiki' variety, as reflected by increases in K232 and K270 values associated with _Ailanthus_ presence, and elevated C18:3 levels under deficit irrigation. However, the combined exposure of olive trees to both stress factors did not produce an additive negative effect, as some oil quality parameters remained unaffected or even showed a slight improvement. Importantly, the measured quality indicators determined in this study at harvest remained within the limits established by the European Union for extra virgin olive oil for these olive oil quality traits. Furthermore, the findings regarding the effects of the storage period on olive oil quality underscore the importance of proper storage conditions to preserve oil quality and prevent oxidative degradation, while also highlighting the critical role of initial oil quality in ensuring successful preservation. Further research is recommended, involving additional olive cultivars and diverse abiotic stress conditions, to fully elucidate the effects of _Ailanthus_ on both tree physiology and oil quality. The following supporting information can be downloaded at: [[https://www.mdpi.com/article/10.3390/app15147678/s1](https://www.mdpi.com/article/10.3390/app15147678/s1)]([https://www.mdpi.com/article/10.3390/app15147678/s1](https://www.mdpi.com/article/10.3390/app15147678/s1)), Figure S1: Effects of time and treatments and analysis of regression lines (the small square within each figure) for: (a) peroxide value; (b) acidity; (c) K232 value; (d) K270 value; (e) AK index; Table S1: Interaction of treatments on quality characteristics of olive oil over time; Figure S2: Effects of time and treatments and analysis of regression lines (the small square within each figure) for: (a) total phenol concentration; (b) total o-diphenyl concentration; (c) flavonoids concentration; (d) antioxidant capacity measured by DPPH method; (e) antioxidant capacity measured by FRAP method; (f) antioxidant capacity measured by ABTS method; Table S2: Interaction of treatments on total phenolics, total o-diphenyl and total flavonoids content and on antioxidant capacity of olive oil according to the DPPH, FRAP and ABTS methods over time; Figure S3: Effects of time and treatments and analysis of regression lines (the small square within each figure) for: (a) C16.0 concentration; (b) C16:1 concentration; (c) C18:0 concentration; (d) C18:1 concentration; (e) C18:2 concentration; (f) C20:0 concentration; (g) C18:3 concentration; (h) C20:1 concentration; Figure S4: Effects of time and treatments and analysis of regression lines (the small square within each figure) for: (a) SFAs concentration; (b) MUFAs concentration; (c) PUFAs concentration; (d) UFAs concentration; (e) SFAs/UFAs ratio; (f) MUFAs/PUFAs ratio; (g) C18:1/C18:2 ratio; (h) squalene concentration; Table S3: Interaction of treatments on fatty acids profile and squalene concentration in olive oil over time. Conceptualization, A.-G.K. and P.A.R. ; methodology, A.-G.K. and P.A.R. ; software, A.-G.K. and P.A.R. ; validation, P.A.R. ; formal analysis, A.-G.K.; investigation, A.-G.K.; resources, P.A.R. ; data curation, A.-G.K.; writing--original draft preparation, A.-G.K.; writing--review and editing, A.-G.K. and P.A.R. ; visualization, A.-G.K.; supervision, P.A.R. All authors have read and agreed to the published version of the manuscript. This research received no external funding. The original contributions presented in this study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding author. The olive trees used in the experiment were donated by G. Kostelenos Nurseries, which is located in Galata, Trizinia. This paper is an extended version of our paper published in [PERSON], A.-G.; [PERSON] Effect of _Ailanthus altissima_ on photosynthetic activity, yield, and olive oil quality characteristics under two irrigation regimes.