## 3 Results and Discussion  
### Morphological and Biochemical Characterization of PGPR  
Based on the observation, all isolates showed creamy white pigmentation. The UPMC1166, UPMC1254 and UPMC1389 strains had irregular shapes: UPMC1168 had a filamentous shape, while the rest (UPMC1376, UPMC1393, UPMC703 and UPMC704) had a circular colony shape. Colonies of UPMC1166 showed raised elevation, undulate margin, bumpy with a shiny appearance, and stained as Gram-positive bacterium with a rod shape. UPMC1168 also showed a unique morphology, with a bumpy and shiny appearance in the middle but dull at the colony's edge. The UPMC1168 also contained a Gram-positive bacterium with a rod shape. The colonies for UPMC1254, UPMC1389, UPMC1376, UPMC1393, UPMC703 and UPMC704 were also raised in elevation, with undulate margin, bumpy and shiny in appearance, and stained as Gram-positive rod-shaped bacterium, except for UPMC1376, UPMC1393 and UPMC704, which had a coccus shape (Table 2). The present colony characterization for UPMC1166 was supported by [PERSON] et al. (2014), who found that the UPMC1166 was a Gram-positive bacterium with a single or in-pair _Bacilli_ arrangement and a rod shape after 24, 48, and 72 h of incubation at 30 \({}^{\circ}\)C. The colonies ranged from 3 to 5 mm in size after 24 h of incubation. After 48 h of incubation, it extended to 5 to 7 mm. During the 48 h observation period, the isolate produced endospores. creamy white coloring, increased elevation, bumpy and shiny appearance, uneven form, translucent and sticky structure and undulate edge, were all characteristics of UPMC1166 colonies. UPMC1166 produced bubbles when H\({}_{2}\)O\({}_{2}\) was applied, showing that it was catalase positive. The morphological properties of UPMC1166, such as Gram-positive bacteria, catalase positivity, rod-shaped cells, and the presence of one endospore in a single cell, matched those of _Bacillus_ species (Bacillus). The form of colonies shows a wide range of variation in within and between _Bacillus_ species, most likely due to the medium component of another incubation environment (Bacillus). The BLAST results revealed that UPMC1166 was intimately connected to _Bacillus atrophaeus_, _B. subtilis_, _B. siamensis_, _B. amyloliquefaciens_, _B. vallismortis_, and _B. mojavensis_, based on molecular identification using 16S rRNA Gene Sequence, because they shared pairwise sequence similarities (Bacillus). All of the isolates were discovered to be rod shaped and tiny in size. Sugar fermentation, oxidase test, urea, TSI, and other biochemical assays were used to identify the isolate. [PERSON] et al. (2013) used the _L. fusiformis_ strain found in soils. According to [PERSON] et al. (2013), a vast group of Gram-positive aerobic (facultatively anaerobic), _Bacillus_ genus, and endospore-forming bacteria are widely distributed in the environment. _Bacil_ can use a variety of simple organic compounds, including sugars, amino acids, carbohydrates, and \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Isolates** & **Shape** & **Pigmentation** & **Margin** & **Form** & **Elevation** & **Appearance** \\ \hline UPMC1166 & Rod-shaped & creamy white & undulate & Irregular & Convex & bumpy, shiny \\ UPMC1254 & Rod-shaped & creamy white & undulate & Irregular & Convex & bumpy, shiny \\ UPMC1168 & Rod-shaped & creamy white & undulate & filamentous & Uphone & bumpy and shiny in the middle, dull in the side \\ UPMC1389 & coccus shaped & creamy white & Entire & Circular & Convex & bumpy, shiny \\ UPMC703 & Rod-shaped & creamy white & Entire & Circular & Convex & bumpy, shiny \\ UPMC1376 & coccus shaped & creamy white & Entire & Circular & Convex & bumpy, shiny \\ UPMC1393 & Rod-shaped & creamy white & undulate & Irregular & Convex & bumpy, shiny \\ UPMC704 & coccus shaped & creamy white & Entire & Circular & Convex & bumpy, shiny \\ \hline \hline \end{tabular} Note: All isolates exhibited positive Gram stain reactions.  
\end{table} 
Table 2: Colony characterization after 24 h of incubation. organic acids. [PERSON] et al.