## 3 Results and Discussion
### Genomic Characteristics
\begin{table}
\begin{tabular}{c c c c} \hline \hline Region & **Type** & **Most Similar Known Cluster** & **Similarity (\%)** \\ \hline Region 2.1 & NRPS & penicillin & 18\% \\ Region 3.1 & T1 PKS, NRPS-like & lucilactaene & 23\% \\ Region 3.2 & T1 PKS & & \\ Region 3.3 & T1 PKS & & \\ Region 3.4 & terpene & squalestatin S1 & 60\% \\ Region 3.5 & NRPS, T1 PKS & equisetin & 18\% \\ Region 3.6 & T1 PKS & andrastin A & 40\% \\ Region 3.7 & NRPS & & \\ Region 3.8 & NRP-metallophore, NRPS & & \\ Region 3.9 & T1 PKS & & \\ Region 3.10 & NRPS-like, T1 PKS & & \\ Region 4.1 & NRPS-like & & \\ Region 4.2 & NRPS-like & & \\ Region 7.1 & T1 PKS & greagatin A & 44\% \\ Region 7.2 & NRPS & & \\ Region 7.3 & terpene & & \\ Region 7.4 & T1 PKS & & \\ Region 7.5 & NRPS & nidulanin A & 75\% \\ Region 7.6 & fungal-RIP-like & & \\  & & & \\  & & & \\ Region 12.1 & indole, NRPS & roqueforine D/roqueforine C/ & 100\% \\  & & & \\  & & & \\ Region 12.2 & NRPS, T1 PKS & & \\ Region 12.3 & NRPS-like & & \\ Region 18.1 & T1 PKS & & \\ Region 20.1 & T1 PKS, indole & & \\ Region 20.2 & NRPS-like & & \\ Region 20.3 & betalactone & & \\ Region 21.1 & NRPS-like, fungal-RIP-like & & \\ Region 21.2 & NRPS & & \\ Region 21.3 & NRPS-like & & \\ Region 21.4 & T1 PKS & & \\ Region 21.5 & terpene & & \\ Region 21.6 & T1 PKS & **4**epi-15-epi-brefeldin A & 20\% \\ \hline \hline \end{tabular}
\end{table} 
Table 3: antiSMASH predicted BGCs in the studied genome. BlastKoala annotated 3949 coding genes (38.9%), with the most relevant results highlighting the species ability for assimilating formaldehyde through the xylulose monophosphate pathway or dihydroxyacetone cycle (methane metabolism and methylotrophy) (M00344; complete); nitrate assimilation (signature module set) assimilating nitrate through the assimilatory nitrate reduction into ammonia (M00615; complete); assimilatory sulfate reduction (sulfur metabolism), converting sulfate to sulfide (H\({}_{2}\)S) (M00176; complete); and the ability of the fungus to synthetize beta-lactams, namely, penicillin, through the pathway aminoadip + cysteine + valine => penicillin (M00672; complete). Methylotrophic microorganisms exhibit the unique capacity to utilize single-carbon substrates, encompassing methane, methanol, formate and carbon monoxide as their primary carbon source for proliferation. The capability for methylotrophic metabolism may provide a considerable benefit, especially in extreme habitats, since they allow these microorganisms to survive and develop in conditions characterized by nutrient scarcity [83; 84]. In fact, fungal methylotrophy could enhance their survival in extreme conditions while also contributing to the detoxification of toxic compounds, the support of carbon cycling and potentially influencing biomineralization processes. On the other hand, in arsenic-rich mine soils, fungal assimilatory nitrate and sulfate reduction might not only promote basic metabolic functions like nitrogen and sulfur acquisition but also contribute to the detoxification and maintenance of cellular homeostasis under extreme environmental stress. Oxidative stress causing the disruption of cellular processes and increase in reactive oxygen species (ROS) can be mitigated by the tight regulation of nitrogen metabolism through antioxidant production, such as glutathione, and by binding arsenic into less toxic forms. Complementarily, assimilatory sulfate reduction ensures the synthesis of cysteine and methionine, critical for the formation of glutathione and allowing for arsenic detoxification, while also permitting the direct interaction of sulfide with arsenic to form insoluble arsenic sulfides (e.g., arsenopyrite), thereby lowering arsenic's bioavailability and toxicity. In fact, from the KEGG analysis for the metabolism of other amino acids, glutathione metabolism was the most represented pathway (17 entries) reinforcing this assumption.