# Method of Optical Diagnostics of Grain Seeds Infected with Fusariumum
## 3 Results  
Two hundred and fifty measurements were carried out with the simultaneous scanning of infected and uninfected seeds. The average results of the measurements of the oat seeds are presented as an example in Figure 1. The values of spectral densities of excitation and luminescence are given in relative units (r. u.) and reduced to 100 units at the maximum sensitivity of the spectrofluorimeter.  
It can be seen from Figure 1 that during infection, the spectral density of absorption capacity increases and the curve \(\eta_{\text{e}}(\lambda)\) shifts upwards in the range of 380-450 nm. In this case, the integral excitation parameter H, calculated by Formula (1), increases by 16.4%. There is a clear shift to the left, and the parameter H increases by 65.8% and 62.1% for the spectra of wheat and barley, respectively. The degree of infection of wheat and barley is 98%.  
For the wavelengths of the excitation maxima \(\lambda_{\text{e}}\) equal to 232, 362, 424, 485 and 528 nm, the luminescence spectra \(\varphi_{\text{f}}(\lambda)\) were measured. Figure 2 shows the spectral characteristics of the luminescence of the oat seeds at different \(\lambda_{\text{e}}\).  
Figure 2 shows that all the spectra of infected seeds are located higher than those of healthy ones. The luminescence flux at \(\lambda_{\text{e}}\) = 232 nm \(\Phi_{232}\) increases significantly (by 1.71 times) during seed infection, but the luminescent signal is very low. When excited by \(\lambda_{\text{e}}\) = 424 nm, the photoluminescence flux increases by 1.30 times. For other excitation wavelengths, the flux increases significantly less--by 1.09-1.15 times. More detailed results for wheat are given in [27].  
Additionally, the photoluminescence spectra of the wheat and barley seeds samples were measured at other degrees of \(\beta\) infection, obtained by mixing healthy seeds from the \(\beta\) = 0 sample and infected seeds from the \(\beta\) = 98% sample in appropriate proportions. The radiation fluxes calculated by Formula (2) are presented in Table 1.  
The dependences of photoluminescence fluxes on the degree of contamination of the sample of barley seeds \(\Phi_{\lambda}\) (\(\beta\)) are shown in Figure 3.  
Figure 1: Excitation spectra of oat seeds during synchronous scanning: 1—uninfected, 2—infected by 82.4%.  
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Degree of Infection with** & \(\mathbf{\Phi_{232}}\)**, r. u.** & \(\mathbf{\Phi_{362}}\)**, r. u.** & \(\mathbf{\Phi_{424}}\)**, r. u.** & \(\mathbf{\Phi_{485}}\)**, r. u.** & \(\mathbf{\Phi_{528}}\)**, r. u.** \\ \hline \multicolumn{5}{c}{Wheat} \\ \hline
0 & 36 & 387 & 833 & 501 & 222 \\
25 & 56 & 490 & 823 & 502 & 240 \\
50 & 62 & 507 & 762 & 514 & 243 \\
75 & 71 & 572 & 729 & 524 & 244 \\
98 & 95 & 556 & 746 & 545 & 264 \\ \hline \multicolumn{5}{c}{Barley} \\ \hline
0 & 21 & 152 & 653 & 694 & 298 \\
25 & 28 & 232 & 638 & 606 & 301 \\
50 & 42 & 243 & 678 & 595 & 280 \\
75 & 45 & 304 & 683 & 592 & 268 \\
98 & 66 & 386 & 870 & 533 & 257 \\ \hline \multicolumn{5}{c}{Oats} \\ \hline
0 & 21 & 586 & 1312 & 652 & 299 \\
25 & 33 & 595 & 1517 & 691 & 319 \\
50 & 35 & 643 & 1534 & 717 & 327 \\
68 & 57 & 701 & 1572 & 726 & 334 \\
82.4 & 36 & 641 & 1706 & 745 & 343 \\ \hline \hline \end{tabular}
\end{table}
Table 1: Photoluminescence fluxes of plant seeds of various degrees of infection with fusarium.