## 1 Introduction  
Glucosinolates (GSLs) constitute a group of sulfur-rich secondary plant metabolites characterized by a variable side chain (R group) derived from amino acids, and a fixed core formed by a S-\(\beta\)-D-glucopyrano unit linked to an O-sulfated (Z)-thiohydroximate function [1]. Their presence has been reported in various tissues of plants belonging to the Brassicales order, with the Brassicaceae family having the highest level of biodiversity with respect to plant species synthesizing GSLs and GSL structures. Recent findings have also revealed their presence in orders other than Brassicales, such as in Malpighiales [2]. GSLs can be classified according to their precursor amino acids in (i) aliphatic GSLs (AGs), deriving from methionine, alanine, leucine, isoleucine, valine, or glutamate, (ii) benzenic GSLs from phenylalanine and tyrosine, and (iii) indole GSLs from tryptophan [3]. AGs represent the most diverse group of GSLs in _Arabidopsis thaliana_ and the main class found in the species of the order Brassicales, especially in Brassicaceae, Capparaceae and Cleomaceae [4]. AGs are constitutively synthesized in the epidermal cells and along the vascular bundles where they contribute to the specialized defense strategies: when activated by the enzymes myrosinase upon tissue damage due to the attack of herbivores or other biotic agents (bacterial and fungal pests), they are hydrolysed to isothiyanates, nitriles and other active molecules. Despite indolic GSLs being usually described as the main GSLs involved in pattern triggered immunity and effector triggered immunity [5], modulations in AGs have also been described in response to biotic [6] and abiotic stress, such as saline and temperature [7; 8; 9]. Moreover, recent evidence depicts GSLs, particularly AGs, as part of the natural leaf recruitment of non-pathogenic bacteria, which may protect plants against disease-causing microorganisms, and as a sulfur reservoir able to counteract sulfur deficiency in the sulfur cycling in plants [10; 11]. AGs show a high structural diversity, since the precursor amino acid undergoes a series of chain elongation steps, giving rise to compounds with different side chain length, known as short- and long-chained, depending on the number of methylene groups. The biosynthetic pathway leading to AGs accumulation has been elucidated first in the model plant _A. thaliana_, followed by other Brassicaceae crops (for a review refer to [PERSON] et al., 2023 [12]), and it can be summarized into three major steps: the chloroplast located amino acid chain elongation (step 1), the core structure assembly (step 2, cytoplasmatic) and the side chain modification (step 3, cytoplasmatic). A complex interplay exists between GSL synthesis, primary metabolism and phytohormones, which affects normal plant growth [12]. As for other secondary metabolisms, GSL biosynthesis is costly for plant cells, and it must, therefore, be tightly regulated, activated and repressed in response to environmental dynamics. Transcription factors (TFs) belonging to the R2R3 class of MYBs have been recognized as both positive and negative transcriptional regulators of genes encoding for GSL-related enzymes [12; 13; 14]. The _A. thaliana_ MYB28, MYB29 and MYB76 were identified as AGs regulatory TFs, MYB28 being the master regulator, while MYB29 showing a minor role, since its expression did not affect biosynthetic gene profiles, and MYB76 exerting a role in transport regulation of AGs, thereby controlling their spatial distribution. Interestingly, the myb28-1 myb29-1 double mutant plant does not accumulate AGs. Moreover, a complex interplay and a both positive and negative combinatory effect among these regulators was shown in _A. thaliana_[15; 16]. Additionally, members of the bHLH family of TFs, namely MYC2, MYC3 and MYC4, were also identified, with the triple knock-out mutant _A. thaliana_ plants (_myc234_) being strongly affected in both indolic and aliphatic GSLs, thereby altering the herbivore susceptibility [17].