## 3 Results and Discussion
### Gene Prediction of Holocellulose Degrading Enzymes in A. olivimuriae
In particular, the number of predicted genes for each family was close to that found in other _Aspergillus_ species, except for acetyl  
\begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline \multicolumn{1}{c}{**Substrate**} & \multicolumn{1}{c}{**Enzyme Activity**} & \multicolumn{1}{c}{**CAZyme**} & \multicolumn{3}{c}{**Number of Protein-Coding Predicted Genes**} \\ \cline{3-8} \multicolumn{1}{c}{} & \multicolumn{1}{c}{**A01**} & \multicolumn{1}{c}{**A1**} & \multicolumn{1}{c}{**A2**} & \multicolumn{1}{c}{**A3**} & \multicolumn{1}{c}{**A4**} & \multicolumn{1}{c}{**A5**} & \multicolumn{1}{c}{**A6**} & \multicolumn{1}{c}{**A7**} \\ \hline \multirow{11}{*}{Cellulose} & \multicolumn{1}{c}{\(\beta\)-1,4-Endoglucanase} & GH3, -5, -6, -7, -9, -12, -45 & 35 (22) & 41 & 42 & 36 & 41 \\  & Cellobiohydrolase & GH7 & 3 (3) & 4 & 3 & 2 & 3 \\  & \(\beta\)-1,4-Glucosidase & GH1, -3 & 17 (11) & 23 & 23 & 22 & 26 \\  & Cellobiose dehydrogenase & AA3, AA8 & 2 (2) & 2 & 2 & 2 & 2 \\  & Lytic polysaccharide & AA9 & 6 (6) & 7 & 9 & 8 & 8 \\  & monooxygenase & AA9 & 6 (6) & 7 & 9 & 8 & 8 \\ \hline \multirow{11}{*}{Hemicellulose} & Xylan & \(\beta\)-1,4-Endoglucanase & GH10, -11, -30 & 6 (5) & 8 & 5 & 6 & 8 \\  & \(\beta\)-1,4-Xylosidase & GH3, -43 & 30 (15) & 36 & 35 & 30 & 43 \\ \cline{2-8}  & \(\beta\)-1,4-Endomannanase & GH5 & 15 (5) & 13 & 15 & 10 & 13 \\  & \(\beta\)-1,4-Anmosidase & GH2 & 6 (2) & 6 & 10 & 6 & 7 \\  & Galactomannan & \(\beta\)-1,4-Galacosidase & GH2, -35 & 9 (3) & 11 & 14 & 11 & 14 \\  & \(\alpha\)-1,4-Galacosidase & GH27, -36 & 3 (2) & 3 & 4 & 2 & 3 \\  & \(\alpha\)-Arabinofuranosidase & GH51, -54 & 3 (3) & 3 & 3 & 5 & 4 \\ \cline{2-8}  & \multirow{4}{*}{Xyloglucuan} & \(\beta\)-1,4-endoglucanase & GH12, -74 & 3 (3) & 6 & 3 & 4 & 4 \\  & \(\alpha\)-, \(\beta\)-Arabinofuranosidase & GH51, -54, -127 & 2 (3) & 3 & 4 & 2 & 3 \\  & \(\alpha\)-Xylosidase & GH31 & 10 (1) & n.a. & 10 1  & \({}^{\rm b}\) & \({}^{\rm b}\) & \({}^{\rm b}\) & \({}^{\rm b}\) \\  & \(\alpha\)-Fucosidase & GH29, -95 & 2 (0) & n.a. & 3 2  & \({}^{\rm b}\) & \({}^{\rm b}\) & \({}^{\rm b}\) \\  & \(\alpha\)-Hashinofuranosidase & GH28 & 3 (1) & 14 & 9 & 22 & 21 \\  & \(\alpha\)-Rhamnosidase & GH78, 106 & 7 (0) & 6 & 8 & 8 & 9 \\  & \(\alpha\)-Arabinofuranosidase, & GH51, -54, -62 & 4 (5) & 5 & 5 & 6 & 6 \\  & \(\beta\)-1,4-Galacosidase & GH2, -35 & 1 (5) & 18 & 15 & 11 & 20 \\  & \(\alpha\)-Arabinofuranasa & GH93 & 3 (2) & 3 & 2 & 1 & 3 \\  & \(\beta\)-1,4-Endogalactanase & GH53 & 1 (1) & 1 & 1 & 1 & 1 \\  & Unsaturated glucuronyl & GH88 & 1 (0) & n.a. & 2  & \({}^{\rm b}\) & \({}^{\rm b}\) & \({}^{\rm b}\) \\ Pectin & Undryase & Unsaturated & CH105 & 2 (0) & n.a. & 3  & \({}^{\rm b}\) & \({}^{\rm b}\) & \({}^{\rm b}\) \\  & \(\beta\)-1,4-Smologlucanase & GH2, -35 & 9 (3) & 11 & 14 & 11 & 14 \\  & \(\beta\)-1,4-Galacosidase & GH2, -35 & 11 (1) & 14 & 11 & 14 \\  & \(\beta\)-1,4-Bmologlucanase & GH38 & 1 (0) & n.a. & 2  & \({}^{\rm b}\) & \({}^{\rm b}\) & \({}^{\rm b}\) \\  & \(\beta\)-1,4-Endoglucanase & GH105 & 2 (0) & n.a. & 3  & \({}^{\rm b}\) & \({}^{\rm b}\) \\  & \(\beta\)-1,4-Bmologlucanase & GH105 & 2 (0) & n.a. & 3  & \({}^{\rm b}\) \\  & \(\beta\)-1,4-Smologlucanase &polygalacturonases, which are significantly lower in number in number in _A. olivimuriae_ (Table 4). The most frequently found glycoside hydrolases were endo \(\beta\)-1,4-glucanase (GH3,GH5, GH6,GH7, GH9, GH12, GH45) followed by \(\beta\)-xylosidase (GH3, GH43). The number of genes related to lignin-modifying enzymes in _A. olivimuriae_ was low and CAZy analysis identified 2, 1 and 3 genes coding for lacase, 1,4-benzquinone reductase and lignin-modifying peroxididases, which, however, is in line with other _Aspergillus_ species [3]. The presence of putative CAZyme-encoding genes in _A. olivimuriae_ genome provides insight into its lignocellulosic-degrading enzyme potential, but cannot be directly related to its real degradation ability.