## 2 Results
### Gene Expression Levels of AsA Biosynthetic Pathways Concerning Tissue Development
In addition, the Myo-inositol pathway was also noted to be more active with _MIOX_1a_ (first 4 h) and ultimately with _MIOX_3a_ (after 4 h), a gene barely detected in _G. max_ (Figure 5), but in salt-stressed roots, it was induced more than 30-fold. In general, these data were significant at \(p\) < 0.05 (Supplementary Table S6). In the case of dehydration, the results of gene expression in leaf transcriptome data from two genotypes, Benning (drought-sensitive) and PI416937 (drought-tolerant), are shown in Figure 7A. Overall, dehydration reduced the mRNA levels of the D-mannose/L-galactose pathway, with no significant difference observed between genotypes. However, dehydration seemed to activate the L-glucose (mainly through _GuILO_1f_ mRNA induction by 40 to 60-fold) and D-galacturonate (_GalUR_1_, \(2\), \(4\), and _5_) pathways early after 6 h, and finally the Myo-inositol pathway by high _MIOX_ transcript (_MIOX_1a_, _1b_, and _3a_) induction at 24 h. In general, both genotypes appeared to respond similarly, but _GalUR_ gene upregulation was more time-intensive in PI416937 (tolerant genotype). In roots, the D-mannose/L-galactose pathway also seemed less dehydration-responsive (or downregulated), while the L-glucose (by _GuILO_1a_, _1g_, and _GME_2a_) and Myo-inositol (_MIOX_2b_) pathways could be activated early at 1 h and the D-galacturonate pathway after 6 h (via _GalUR_1_, \(2\) and _5_) (Figure 7A). Mostly, these data were significant at \(p\) < 0.05 (Supplementary Table S6). For drought, leaves and roots were analyzed after 5 and 6 days of stress exposure and 1 day of recovery (Figure 7A). In leaves, the gene expression of AsA biosynthesis pathways appeared mostly unaffected after a longer duration of stress exposure, while in roots, stress responses were more evident. Despite this unremarkable effect of drought in leaves, recovery largely stimulated genes from the D-mannose/L-galactose pathways. In roots, AsA biosynthesis genes from D-mannose/L-galactose were dominant and significantly downregulated at 5 and 6 days of drought; however, the L-glucose (by _GuILO_1c_, _1d_, _1f_, and _GME_2a_), D-galacturonate (by _GalUR_2_ and _5_), and Myo-inositol (_MIOX_1b_) pathways could be activated, despite some downregulated genes in these pathways. Interestingly, recovery in roots generally reversed the downregulation or upregulation of AsA genes, indicating a vital drought response. Overall, these data were significant at \(p\) < 0.05 (Supplementary Table S6). In submergence, leaves and roots exposed to stress for 1 to 3 days and recovery (1 day) revealed peculiar up-and downregulated genes (Figure 7B). In leaves, although most of the genes from the D-mannose/L-galactose pathway were downregulated, certain genes, such as _GMP_2a_ and _alpha_C_, _GPP_1_, _GME_1b_ and _2b_, and _GalLDH_ (_1a_ and _1b_), could still contribute to maintaining the activation of this pathway. However, the other pathways appeared to be more activated with the upregulation of key genes, such as _GuILO_1f_ and _1g_ for L-glucose, _GalUR_1_, \(2\), \(4\), and \(5\) for D-galacturonate, and _MIOX_1a_, _1b_, and _2b_ for the Myo-inositol pathway. In roots, the large majority of genes were severely downregulated compared to leaves. Only _GuILO_1f_ and _MIOX_3a_ were upregulated. Curiously, recovery in leaves and roots typically reversed the down- and upregulation of AsA genes. The differential expression of most of the genes was found to be significant at \(p\) < 0.05 (Supplementary Table S6). Expression Level of Genes Associated with AsA Biosynthesis in Leaves and Roots Under Biotic Stresses  
Figure 8 illustrates the expression levels of genes linked to AsA biosynthesis in the leaves and roots of \(G\). _max_ during exposure to different biotic stresses.