## 3 Results  
### EO and FW Chemical Composition Evaluation with GC/MS  
The chemical composition of the EOs obtained from the \"_Nanuk_\" marigold variety in all three years is presented in Table 1.  
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Compound** & **Molecular** & **RI** & **2018 \({}^{*}\)** & **2019 \({}^{*}\)** & **2020 \({}^{*}\)** \\
**Name** & **Formula** & **(Area \%)** & **(Area \%)** & **(Area \%)** & **(Area \%)** \\ \hline D-Limonene & C\({}_{10}\)H\({}_{16}\) & 14.01 & 4.42 & 5.35 & 7.83 \\ \hline \(\beta\)-Ocimene & C\({}_{10}\)H\({}_{16}\) & 15.99 & 5.28 & 5.57 & 11.73 \\ \hline Carene & C\({}_{10}\)H\({}_{16}\) & 17.54 & 9.93 & 11.21 & 15.45 \\ \hline Oxirane & C\({}_{2}\)H\({}_{4}\)O & 23.51 & 3.02 & nd & 6.25 \\ \hline \(\beta\)-Pinocamphone & C\({}_{10}\)H\({}_{16}\)O & 24.63 & 3.73 & nd & nd \\ \hline Ciclohexene methanol & C\({}_{7}\)H\({}_{14}\)O & 24.66 & 3.08 & nd & 3.13 \\ \hline _cis_-Tagetone & C\({}_{10}\)H\({}_{16}\)O & 24.71 & nd & nd & 2.41 \\ \hline _trans_-Tagetone & C\({}_{10}\)H\({}_{16}\)O & 25.13 & nd & 2.71 & 4.61 \\ \hline \(\alpha\)-Pinocamphone & C\({}_{10}\)H\({}_{16}\)O & 25.38 & 7.90 & nd & nd \\ \hline Linalool & C\({}_{10}\)H\({}_{18}\)O & 26.02 & nd & 4.27 & 2.34 \\ \hline Azulene & C\({}_{10}\)H\({}_{8}\) & 26.93 & nd & 3.10 & nd \\ \hline Caryophyllene & C\({}_{15}\)H\({}_{24}\) & 26.74 & 12.69 & 13.38 & 3.45 \\ \hline Estragole & C\({}_{10}\)H\({}_{12}\)O & 28.98 & nd & 13.58 & 4.09 \\ \hline _cis_-Verbenone & C\({}_{10}\)H\({}_{14}\)O & 29.13 & 5.44 & 4.23 & nd \\ \hline Germacrene & C\({}_{15}\)H\({}_{24}\) & 29.44 & 6.12 & 6.97 & nd \\ \hline _trans_-Verbenone & C\({}_{10}\)H\({}_{14}\)O & 29.58 & 10.18 & nd & 9.81 \\ \hline Eudesmadiene & C\({}_{15}\)H\({}_{24}\) & 30.89 & nd & 3.09 & nd \\ \hline Piperitone & C\({}_{10}\)H\({}_{16}\)O & 29.86 & 3.77 & 9.01 & 1.36 \\ \hline Elemene & C\({}_{15}\)H\({}_{24}\) & 30.00 & 4.20 & 2.53 & 3.05 \\ \hline Berbenone & C\({}_{10}\)H\({}_{14}\)O & 34.27 & 9.37 & nd & nd \\ \hline Piperitenone & C\({}_{10}\)H\({}_{14}\)O & 34.07 & 8.49 & 6.56 & 8.73 \\ \hline
3-Eicosyne & C\({}_{20}\)H\({}_{38}\) & 34.55 & nd & 4.55 & nd \\ \hline Phytol & C\({}_{20}\)H\({}_{40}\)O & 35.00 & nd & nd & 1.48 \\ \hline Elemol & C\({}_{15}\)H\({}_{26}\)O & 37.22 & 2.38 & nd & nd \\ \hline Cadinol & C\({}_{15}\)H\({}_{26}\)O & 39.30 & nd & 4.88 & 1.28 \\ \hline Total of major compounds & \multicolumn{4}{c}{25 compounds identified, representing over 99.99\%} \\ \hline \hline \multicolumn{4}{c}{Classes} \\ \hline \hline  & Monoterpene & 19.63 & 25.23 & 35.01 \\ hydrocarbons & 19.63 & 25.23 & 35.01 \\ \hline  & Oxygenated & 48.87 & 40.36 & 46.34 \\  & Monoterpenes & 68.50 & 65.59 & 81.35 \\ \hline  & Sesquiiterpene & 23.01 & 24.97 & 6.50 \\ \hline  & Oxygenated & 2.38 & 4.88 & 1.28 \\  & Sesquiiterpenenes & 25.39 & 29.85 & 7.78 \\ \hline  & Diterpene & 0 & 4.55 & 0 \\ \hline  & Oxygenated & 0 & 0 & 1.48 \\ \hline \end{tabular}
\end{table}
Table 1: Chemical composition of the EOs (period 2018–2020) isolated from the aerial plant part of the marigold (_T. patula_ L., fam. _Asteraceae_).  
The analyses conducted with GC-MS over three years (2018-2020) on FWs identified, on average, 16 compounds, representing between 99.99% and 100% of the total compounds separated. Carene (9.93-15.45%), cyanophyline (3.45-13.38%), piperitone (6.56-8.73%), piperitone (1.36-9.01%), and elemene (2.53-4.20%) were the main compounds identified. Additionally, other compounds were identified in smaller quantities, such as D-limonene (4.42-7.83%), \(\beta\)-ocimene (5.28-11.73%), etc. (Table 1).  
Regarding the compound classes, the following were identified: monoterepenes (65.69-81.36%), especially oxygenated monoterepenes (40.36-48.88%), along with sesquiterpenes (7.78-29.85%), particularly sesquiterpene hydrocarbonate (6.50-24.97%). Additionally, diTPenes were identified, more notably in 2019 (4.55%).