## 1 Introduction  
In the 21 st century, nanotechnology is an interdisciplinary branch of science, describing structures sized 100 nm or less, allows characterizing innovative possibilities of using nano-scale metals with biological properties important for the industry [1, 2, 3]. Metal nanoparticles find interdisciplinary applications in various fields, e.g., silver is used in the production of food packaging, cosmetics or medical bandages [4, 5, 6]. The synthesis of silver nanoparticles can be physical, chemical or biological. The type of the used method influences the properties of the obtained nanostructures [7, 8]. One of the most desirable properties exhibited by metal nanoparticles seems to be antimicrobial activity resulting from the shapes assumed in the structure of the carrier in which they are embedded. This biological activity described in the literature is based on the interaction of nanostructures with microbial cell surfaces, influencing metabolic processes in microbial cells, which in turn lead to the destruction of internal structures, inhibition of microbiota development and microbial contamination [9; 10; 11; 12]. A special example of a carrier stabilizing the formation of silver nanoparticles are substances of natural origin, which show innovative properties that allow the creation of plastic gels. Biopolymers as biodegradable and intranasalable substances have the advantage of low toxicity to the ecosystem because they quickly decompose into basic compounds. Among such substances, examples of sodium alginate or chitosan can be cited [13; 14; 15; 16; 17]. Sodium alginate is a biopolymer consisting of D-mannuronic and L-galuronic acid. In the food industry, it is used to create modern food packaging. As a substance derived from the natural environment, it is a non-toxic matrix for the creation of components containing active biological factors, e.g., silver nanoparticles [18; 19]. On the other hand, chitosan is a biodegradable polymer used in the food industry and bioengineering for the process of sprinkling active substances. When using chitosan, it is possible to create components containing biologically active factors [20; 21; 22]. Tomato (_Solanum lycopeneum_) is a popular vegetable belonging to the _Solanaceae_ family. Tomato fruits are an important part of the human diet due to the wide range of bioactive compounds such as carotenoids, flavonoids, antioxidants and anti-cancer compounds [23; 24; 25]. The production of tomato fruit is one of the largest undertakings in the world. In 2018, it reached 182 million tonnes. The largest tomato producing countries are the United States, China and Turkey, while Spain and Italy are the largest tomato producers on the European market [26]. The tomato is also an example of a model organism [27] and can be used in a variety of in vitro experiments [28; 29; 30]. In vitro cultivation requires prior preparation of the plant material-sterilisation. If the culture is carried out from seeds, surface sterilisation of the seeds is usually applied [31]. Many methods of surface sterilisation of seeds are known [32; 33], but they are not always effective. Many authors observed culture contamination despite the use of surface sterilisation and high care during handling or with prolonged treatment with disinfectants, lower levels of germination and seedling survival were observed [32]. The cause of such phenomenon may be microorganisms located inside the seeds [34]. Plant organisms, including the tomato (_Solanum lycopeneum_), can become a reservoir for many strains of microorganisms. These microorganisms can survive inside the seed structure of dormant seeds and, as a result of the germination process, move to the newly formed seedling. Potential microorganisms that inhabit the plant are bacteria of the genus Bacillus, Pseudomonas, Acinetobacter and Staphylococcus. The large diversity of plant microbiota results from changes in the environment in which the plant grows [28; 35; 36]. Infection of the culture may be observed after seed rupture--germination begins.