## 3 Results and Discussion
### External Representative ABFPs on the Representative Antibioticfilm HSPN  
In a recent work, [PERSON] et al. arrived at 14 representative ABFPs out of a total of 51 peptides with a reported antibofilm activity either through inhibition of the biofilm formation or through the eradication of pre-formed biofilms. The selection was based on the identification of 14 ABFP classes according to their mechanisms of action. They also evaluated them against the biofilm and planktonic forms of Gram-positive bacterium Streptococcus mutans, the Gram-negative bacterium Pseudomonas aeruginosa, and the fungus Candida albicans. Those ABFPs with minimal biofilm inhibitory concentrations (MBICs) that are lower than their minimal inhibitory concentration (MICs) represented promising candidates against biofilm-related infections. Table 4 shows the 14 representative candidates categorized by their antibofilm mechanisms of action (information taken from Table S2 published in [69]). This set made up of 14 ABFP classes representing different mechanisms of action for carrying out antib biofilm activity was mapped on the structural/chemical space of the 66 ABFPs, drawn by the HSPN model (Figure 9A). As the subset of 66 ABFPs showed the \begin{table}
\begin{tabular}{c c c} \hline \hline
**Class-Peptides** & **Action Mode** & **Sequence** \\ \hline \multirow{2}{*}{Class1- HBD-3} & \multirow{2}{*}{Influences _iaxAD_ and _iaxR_ genes’ transcription levels} & GINITLQKYKYKYRYRGCRCVLSCLP \\  & & KEEQIGKCSTGRKCCKRKK \\  & & KTBSLCTPGCKTGALM \\  & & GCNNMKTATCNSHVSK \\  & & RKSYKCLHKRCR \\  & & KRFKKFFKKLKNSVKK \\  & & FPF \\ \multirow{2}{*}{Class5- Pleurocidin} & \multirow{2}{*}{\begin{tabular}{c} Induces disturbance/permeabilization of the membranes and \\ bindssss to bacterial DNA, causing interference with cellular \\ functions \\ \end{tabular} } & GWGSFFKKAAHVGK HVGKAALTHYL \\  & & \\ \multirow{2}{*}{\begin{tabular}{c} Class6- Pac-525 \\ \end{tabular} } & \multirow{2}{*}{\begin{tabular}{c} Able to enter membranes and to affect the \\ lipopolysaccharideaccharides of Gram-negative bacteria \\ Decreases the attachment of bacterial cells, stimulates \\ twitching motility, and influences two major quorum sensing \\ systems \\ \end{tabular} } & KRFRIVRV \\  & & \\ \multirow{2}{*}{\begin{tabular}{c} Class8- Indolicidin \\ \end{tabular} } & \multirow{2}{*}{\begin{tabular}{c} Induces lipid removal and mixed indolicdin-lipid patches \\ alongside membrane permeabilization \\ Forms amyloid fibers to associate with the bacterial \\ membrane and produce transmembrane pores \\ \end{tabular} } & ILPWKWPWPWR \\  & & \\ \multirow{2}{*}{\begin{tabular}{c} Class9- Protegrin-1 * \\ Class10- Peptide 3002 \\ Class11-TetraZW-RR * \\ \end{tabular} } & \multirow{2}{*}{\begin{tabular}{c} Forms amyloid fibers to associate with the bacterial \\ membrane and produce transmembrane pores \\ Blocking (p)pGPpp \\ Disrupts membranes to kill bacteria rapidly \\ \end{tabular} } & RGGRLCYCKRRFCVCVGR \\  & & \\ \multirow{2}{*}{\begin{tabular}{c} Class12-P1 \\ \end{tabular} } & \multirow{2}{*}{\begin{tabular}{c} Disrupts membranes to kill bacteria rapidly \\ interferes with the proper section and/or intermolecular \\ interaction of key extracellular polymers in the biofilm matrix \\ LPS-binding property interferes with bacterial attachment, \\ destroying the bacterial membrane \\ \end{tabular} } & ILVRWYRRVWRRVCWYRRV \\  & & \\ \multirow{2}{*}{\
\begin{tabular}{c} Class14-Melittin * \\ \end{tabular} } & \multirow{2}{*}{
\begin{tabular}{c} Inhibits the expression of biofilm-associated _hap_ genes \\ \end{tabular} } & GIGAVLKVLTTGLPLASWIKKRQQ \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Representative ABFPs categorized in 14 classes according to the mechanisms of action elucidated for their antibiofilm activity. The information displayed in this table was gathered from Table S2 published in ref. [69]. best representativeness of the antibiofilm chemical space, it was used along with the most suitable HSPN projection for overlapping purposes.