###### Abstract  
This study investigated the ability of aqueous leaf extracts of _Heliotropin luciferum_ (HE), _Ocimum duflaerense_ (OE), and _Zataria multifoma_ (ZE) to detoxify aflatoxin BI (AFB1) under in vitro and in vivo conditions. The results showed that HE, OE, and ZE degraded 95%, 93%, and 92% of AFB1, respectively, after 72 h incubation at 37 \({}^{\circ}\)C. The degradation of AFB1 was validated by liquid chromatography-mass spectrometry analysis. A molecular ion peak at \(m/z\) 313 specific to AFB1 (C\({}_{2}\)H\({}_{2}\)O\({}_{6}\)) was observed in the mass spectrum of untreated AFB1 (control). However, the level of AFB1 was decreased to untraceable levels in response to treatment with these plant extracts. HE, OE, and ZE effectively detoxified AFB1 in a concentration-dependent manner, resulting in mortality rates of 65, 70, and 75% of brine shrimp, respectively, in contrast to 90% in the untreated AFB1 (control). The hepatoprotective effect of HE, OE, and ZE against AFB1-induced oxidative stress pathogenesis was investigated using mice as an experimental model. Glutathione depletion, impairment of total antioxidant capacity, and increase in DNA oxidative damage were observed in liver tissues of mice treated with AFB1. However, HE, OE, and ZE extract supplementation suppressed the oxidative damage associated with AFB1 treatment. Our findings indicated that HE, OE, and ZE were highly effective in the detoxification of AFB1. In addition, HE, OE, and ZE act as potent antioxidants and combat the AFB1-associated oxidative stress and liver pathogenesis, suggesting that these plants might be valuable for the development of functional foods aimed at minimizing the toxic effects of AFB1. degraded aflatoxin BI products; biological detoxification; food safety; herbal extracts; liquid chromatography-mass spectrometry; mycotoxins 2020 2020  
phases and produce aflat [5]. Ingestion of food contaminated with aflatoxin causes a disease called \"aflatoxicosis\" in humans [6]. So far, 21 varieties of aflat have been detected [7]. Aflatoxin G1 (AFG1; MW 328.0578), aflatoxin G2 (AFG2; MW 330.2889), aflatoxin B1 (AFB1; MW 312.2736), and aflatoxin B2 (AFB2; MW 314.2895) are common contaminants in agricultural products [8]. Aflatoxin M1 (AFM1; MW 328.0577), the principal metabolite of AFB1, is often present in the milk of lactating animals that have ingested AFB1-contaminated feed [5]. \"AFB1 (C17H12O6)\" is recognized as the most serious carcinogenic mycotoxin to humans and animals [9; 10]. Several nations have specified maximum tolerance limits for aflat in food for human consumption to safeguard the population from the risk of aflatoxin [11]. The Food and Drug Administration in the USA and the European Union have set regulatory limits of 20 ppb and 4 ppb for foods, respectively [12]. Aflatoxins cannot be completely removed or degraded from contaminated agricultural commodities due to their stability. Currently, a few biological, physical, and chemical approaches are used to detoxify food items containing aflat [13; 14; 15; 16]. However, each process has downsides, because the treated foods must be safe for human consumption and their nutritional value must not be changed. Detoxification of aflat using plant products is an effective, biologically safe, and practical approach to reduce their toxic effects on humans. Extracts of _Trachysperum annuli_[17], _Ocimum tenuniform_[18], _Ocimum busilicum_[19], _zimmu_ (_Allium sativum \(\times\) A. cepa_) [20], _Barleria lupulina_[21], _Aflatoda suica_[22], _Centella asatica_, _Eclipa prostrata_, and _Hybanthus ennespermus_[23] have been used for the detoxification of aflat. In the Sultanate of Oman, the existence of more than 250 species of medicinal plants has been reported. These traditional medicinal plants may be potential tools for the biological detoxification of aflat.