## 3 Results  
### Identification of Bioactive Compounds  
GC-EAD analysis of cracked pomergnate volatiles revealed at least 11 peaks that generated an EAG response (Supplementary Materials S5). Table 1 summarizes candidate molecules and associated identification criteria. Note that the table also includes some green leaf volatiles that did not generate an electrophysiological response but were considered valuable in the context of this investigation (see the Discussion Section). Last but not least, definitive confirmation of component identity, using both retention and spectral verification, was achieved for \(\beta\)-caryophyllene, i.e., the component with the most consistent response in the GC-EAD analyses, by analyzing an authentic standard solution containing the native component.  
\begin{table}
\begin{tabular}{c c c c c c c c c c c} \hline \hline \multirow{2}{*}{**Peak**} & **RT** & \multirow{2}{*}{**EAD**} & \multirow{2}{*}{**Candidate**} & **TIC** & \multirow{2}{*}{**HRF 2**} & **RI 3** & \multirow{2}{*}{**ARI 4**} & \multirow{2}{*}{**PCI 5**} & \multirow{2}{*}{**A\({}_{\text{ppm}}\)6**} & \multirow{2}{*}{**FISH 7**} \\  & **(min)** & & & & **(Area/10\({}^{\text{\#}}\))** & & & & & & \\ \hline
1 & 6.26 & N & Hexanal & 9.79 & C6H12O & 98.8 & n/a \({}^{*}\) & n/a & + & 0.45 & + \\
2 & 7.06 & N & _trans_-3-Hexen-1-ol & 5.69 & C6H12O & 99.3 & 834 & 18 & - & n/a & + \\
3 & 7.53 & N & _cis_-3-Hexen-1-ol & 496 & C6H12O & 99.8 & 861 & 4 & - & n/a & + \\
4 & 9.48 & Y & \(\alpha\)-Pineine & 969 & C10H16 & 98.3 & 945 & 3 & + & \(-\)0.67 & + \\
**5** & **34.08** & **Y** & \(\beta\)**-**Caryophyllene** & **244** & **C15H24** & **99.9** & **1424** & **5** & **+** & **0.78** & **+** \\
6 & 34.75 & Y & _cis_- Berganometere & 3110 & C15H24 & 99.7 & 1435 & 20 & + & 0.65 & + \\
7 & 35.16 & Y & _cis_-\(\beta\)-Cedrene & 455 & C15H24 & 99.7 & 1442 & 21 & + & 0.57 & + \\
8 & 35.78 & Y & _cis_-\(\beta\)-Farmesene & 468 & C15H24 & 99.8 & 1452 & 12 & + & 0.48 & + \\
9 & 35.92 & Y & Isogermacrene D & 468 & C15H24 & 99.7 & 1455 & 7 & + & 0.09 & + \\
10 & 36.23 & Y & \(\alpha\)-Humuliene & 574 & C15H24 & 99.8 & 1460 & 6 & + & 0.49 & + \\
11 & 37.83 & Y & \(\beta\)-epi(-E) & 478 & C15H24 & 99.8 & 1486 & 8 & + & 0.21 & + \\
12 & 38.67 & Y & Pentadecane ** & 1380 & C15H32 & n/a & 1500 & 0 & - & n/a & + \\
13 & 39.18 & Y & Valencene & 438 & C15H24 & 99.5 & 1509 & 9 & + & \(-\)0.03 & + \\
14 & 39.95 & Y & Ethly-4 ethoxy & 916 & C11H14O3 & 98.0 & 1523 & 1 & + & \(-\)0.28 & + \\ \hline \hline \end{tabular} n/a = not available. \({}^{*}\) RI could not be calculated using the C8–C20 alkane mix because the compound elutes before C8. ** Component used as internal standard. \({}^{1}\) Molecular formula of the candidate compound. \({}^{2}\) High-resolution filtering score as determined by Compound Discoverer. \({}^{3}\) Experimental Retention Index (RI) as determined by Compound Discoverer. \({}^{4}\) Difference between experimental RI and tabulated RI for a similar column in NIST 2020. \({}^{5}\) Result of Positive Chemical Ionization analysis. \({}^{*}\)+ means that the molecular formula as depicted in the MF column was confirmed. \({}^{6}\) Mass accuracy in ppm as calculated by Compound Discoverer for the molecular ion in the PCI analysis. \({}^{7}\) Fragment Ion Search score as determined by Mass Frontier 8.1.  
\end{table}
Table 1: Component identification from cracked pomergnate headspace extract by means of GC-Orbitrap MS workflow.