## 4 Discussion
It was proposed that PMMA denture disinfection achieved by microwave irradiation is due to the presence of distilled water, which forms bubbles upon boiling, thus removing microorganisms from the sample surface [36; 37]. A study by [PERSON] et al. revealed that microwave irradiation at reduced power can be used for a high level of disinfection, but not for sterilization [22], which is in line with our findings. In our study, fungal cell adhesion was analyzed by a crystal violet binding assay, and cell count was analyzed by an aerobic plate count assay. The plate count showed that the control acrylic slide carried 1400 cfu/mL of _C. albicans_. The results show that the subject isolate of _C. albicans_ showed a high level of antimicrobial resistance against denture cleaning tablets (sodium perborate). This was attributed to biofilm formation, as _C. albicans_ nullified the toxic effect of sodium perborate and survived even after 5 min of exposure. This agreed with the findings of the previous studies by [PERSON] et al. and [PERSON] et al. [32; 38]. They both identified that alkaline peroxide denture cleansers are effective against the streptococcus species and do not show much activity against _C. albicans_. This also agrees with the study by [PERSON] et al., who studied the effect of a denture cleaning tablet (Bonyplus) against _Candida_ species and _S. mutans_ and found that the denture cleansing solution did not show significant re-educating of _Candida_ species [39]. In addition, the alkaline peroxide tablets were not effective against _S. mutans_. Interestingly, samples were fabricated in our study by mixing powder and liquid. However, CAD-CAM dentures are also available, fabricated from prepolymerized PMMA resin blocks [40]. The prepolymerized resin dentures allow for fewer surface defects and irregularities, and the incidence of fungal infection among these dentures and their disinfection protocol may differ from conventional PMMA dentures. Therefore, studies assessing the disinfection of CAD-CAM PMMA dentures are warranted. Our study showed higher disinfection efficacy of MW with sodium perborate-disinfection tablets for the use of _Candida_ in comparison with microwave (MW) and cleaning tablets (DC) alone. It is pertinent to mention that MW disinfection of PMMA denture bases can also result in distortion and discrepancy in their fit and adaptation on stone casts [17]. However, [PERSON] et al. showed that at high MW energy, distortion was observed for the PMMA denture, while at 500 W or below, the dimensional accuracy was comparable to the control [17]. As MW disinfection in our study was performed at 450 W power, the PMMA resin specimens when exposed to our study's protocol for disinfection were dimensionally accurate and therefore could be clinically utilized. In addition, our study displayed some inherent limitations. Primarily, the study was based on in vitro design, assessing a single species of _Candida_, which may have limited the clinical implications. Moreover, only one type of denture disinfecting tablet was used; however, cleansing agents with hydrogen peroxide, sodium hypochlorite, and Chlorhexidine are also available. Therefore, further studies assessing the different candida species with contemporary denture cleansing agents are warranted to investigate the antimicrobial efficacy of microwave irradiation.