## 3 Results
### Exoproteomes Analysis During Semi-Solid Cultivation on Media Containing Various Types of Sawdust
\begin{tabular}{c c c c} \hline
**Spott Protein ID** & **HH description** & **EC** & **CA2Y** & **Signal** & **GP-B** & **GP-A** & **GP-B** \\
**Plant Cell Wall** & & & & \\
1 & 17K236273 & Aryl-alcohol oxidase-like protein (AAO) & & & & \\
2 & 17K236011 & Lacose & & & & \\
3 & 17K3375363 & Maluse-glucose samples & & & & \\
4 & 17K343143 & Lacose & & & & \\
5 & 17K361917 & Aryl-alcohol oxidase-like protein (AAO) & & & & \\
6 & 17K34033 & Aryl-alcohol oxidase-like protein (AAO) & & & & \\
7 & 17K342033 & Aryl-alcohol oxidase & & & & \\
8 & 17K34203 & Aryl-alcohol oxidase & & & & \\
9 & 17K34203 & Aryl-alcohol oxidase & & & & \\
10 & 17K342319 & Aryl-alcohol oxidase monozygense (IPMO) & & & & \\
11 & 17K432031 & Aryl-alcohol oxidase monozygense (IPMO) & & & & \\
12 & 17K420274 & Sohn/odds & & & & \\ \hline \hline \end{tabular} 
\begin{tabular}{c c c} \hline
**Fungal cell wall** & & & & \\
13 & 17K342031 & 1.3(-beta-)-glucose sample1 GA & & & \\
14 & 17K342032 & Aryl-alcohol-2,3-beta-glucose samples & & & & \\
15 & 17K34203 & 1.3(-beta-)-glucose/bre
Proteases of different families as well as a large number of proteins with unknown functions were also found in the secretomes of _C. leave_ (Figure 4). In the case of the latter, these are mainly low molecular proteins (10-25 kDa) with a large number of serine/glycine/cysteine repeats that is typical for various adhesins and hydrophobins. Preliminary Metabolome Analysis During Semi-Solid Cultivation on Media Containing Various Types of Sawdust  
At the end of _C. leave_ cultivation, the content of reducing sugars on the GP and the GP-P media was lower than initial sugar concentration (Table 2). On the contrary, after the fungal cultivation, the reducing sugars content on the GP-B and the GP-A media was higher than at the beginning of cultivation. Preliminary GC-MS metabolic analysis was carried out using the standard derivatization scheme which showed that the composition of _C. leave_ metabolomic profile was dependent on a culture medium (Supplementary Figures S4-S7, Supplementary Tables S1-S4). It was found that the major low molecular weight metabolites in the fungal CLs samples were, predominantly, monosaccharides and carboxylic acids. Notably, the levels of monosaccharides and polyols in a total pool of identified metabolites increased on \begin{table}
\begin{tabular}{c c c c c} \hline \multirow{2}{*}{**Culture Liquid**} & \multicolumn{2}{c}{**Total Phenols, mg/mL**} & \multicolumn{2}{c}{**Reducing Sugars, mg/mL**} \\ \cline{2-5}  & **Control** & **After Cultivation** & **Control** & **After Cultivation** \\ \hline GP & 89.6 \(\pm\) 0.9 & 48.9 \(\pm\) 1.5 & 0.45 \(\pm\) 0.05 & 0.36 \(\pm\) 0.03 \\ GP-B & 202.3 \(\pm\) 4.3 & 75.7 \(\pm\) 0.7 & 0.45 \(\pm\) 0.07 & 0.59 \(\pm\) 0.06 \\ GP-A & 197.3 \(\pm\) 3.2 & 48.8 \(\pm\) 0.2 & 0.50 \(\pm\) 0.03 & 0.64 \(\pm\) 0.05 \\ GP-P & 129.8 \(\pm\) 3.4 & 53.7 \(\pm\) 0.5 & 0.41 \(\pm\) 0.04 & 0.29 \(\pm\) 0.03 \\ \hline \end{tabular}
\end{table} 
Table 2: Phenols and reducing sugars content in culture liquids after 30 days of _C. leave_ cultivation. Figure 5: Distribution profile of identified secreted proteins and the distribution of CAZymes (GHs, AAs and CEs classes) in the GP (glucose-peptone medium), GP-B (glucose-peptone with birch sawdust), GP-A (glucose-peptone with alter sawdust) and GP-P (glucose-peptone with pine sawdust) of the _Crucibulum leave_ LE-BIN 1700 secretomes. AA—auxiliary activity enzyme; CE—carbohydrate esterase; GH—glycos hydrolase. the sawdust-containing media (Figure 6, Supplementary Tables S1-S4). Glucose, galactose, mannose, xylose, arabinose, rhamnose and ribose were detected on the sawdust-containing media. The GP-B medium also contained the disaccharides \(\alpha\)-(1\(\rightarrow\)2)- and \(\alpha\)-(1\(\rightarrow\)3) mannobiose, which are part of the fungal cell wall. Among the polyols identified, glycerol and erythroid were the predominant compounds on the sawdust-containing media; 1,5-anhydro-D-sorbitol was additionally detected on the GP-B medium.