## 3 Results
### Photoproduction Activity  
A dose-response evaluation of each extract was performed in a fibroblast cell line, 3T3, using a range of concentrations between 0.01 and 4 mg\(\cdot\)mL\({}^{-1}\), in order to evaluate the security of the extracts for skin applications (Figure S1). E1 and E2 (extraction with 50% ethanol/50% water) were the extracts with cell toxicity associated with more concentrations, especially E2, where cell viability was above 80% only in two of the eight concentrations tested. For E4, no concentration revealed a cytotoxic effect on 3T3 when compared with the control, and for E3 and E6, only the highest concentration (4 mg\(\cdot\)mL\({}^{-1}\)) was responsible for  
\begin{table}
\begin{tabular}{c c c c c c c c c c c c c c} \hline \hline  & \multicolumn{10}{c}{_Staphylococcus aureus_} \\ \hline  & \multicolumn{2}{c}{**E1**} & \multicolumn{2}{c}{**E2**} & \multicolumn{2}{c}{**E3**} & \multicolumn{2}{c}{**E4**} & \multicolumn{2}{c}{**E5**} & \multicolumn{2}{c}{**E6**} & \multicolumn{2}{c}{**C + (Tetracycline)**} \\ \hline mg\(\cdot\)mL\({}^{-1}\) & Mean & SD & Mean & SD & Mean & SD & Mean & SD & Mean & SD & Mean & SD & Mean & SD \\ \hline
**3** & 17.22 & 5.25 & 37.77 & 5.84 & 54.01 & 1.48 & 28.37 & 4.50 & 32.40 & 7.95 & 35.80 & 4.68 & 100.56 & 0.26 \\
**1.5** & 15.14 & 4.36 & 26.88 & 5.85 & 52.99 & 3.67 & 25.60 & 4.41 & 23.72 & 6.71 & 24.90 & 7.99 & 101.18 & 1.23 \\
**0.75** & 29.58 & 8.93 & 35.13 & 8.60 & 52.64 & 3.66 & 38.33 & 2.88 & 10.79 & 21.08 & 4.28 & 23.15 & 100.54 & 0.22 \\
**0.0075** & \(-\)8.93 & 2.33 & \(-\)3.49 & 3.31 & \(-\)9.23 & 5.29 & \(-\)5.86 & 5.99 & \(-\)10.58 & 6.05 & \(-\)10.63 & 5.72 & 100.23 & 1.02 \\ \hline  & \multicolumn{10}{c}{_Escherichia coli_} \\ \hline  & \multicolumn{2}{c}{**E1**} & \multicolumn{2}{c}{**E2**} & \multicolumn{2}{c}{**E3**} & \multicolumn{2}{c}{**E4**} & \multicolumn{2}{c}{**E5**} & \multicolumn{2}{c}{**E6**} & \multicolumn{2}{c}{**C + (Ciprofloxacin)**} \\ \hline mg\(\cdot\)mL\({}^{-1}\) & Mean & SD & Mean & SD & Mean & SD & Mean & SD & Mean & SD & Mean & SD & Mean & SD \\ \hline
**3** & 21.14 & 1.70 & 25  
a reduction in cell viability of close to 80% (\(p<0.0001\)), with all the other concentrations being above 80% of cell viability. E5 showed significant differences when compared to the control (\(p<0.0001\)) for the concentrations of 2 and 4 mg\(\cdot\)mL\({}^{-1}\), but also kept the values of cell viability near to 80%. Mostly in extracts with a lower or no concentration of ethanol in the extraction procedure (E3-E6), it was also possible to verify that the lowest concentrations tested were responsible for an increase in lysosomal activity, which might indicate growth promotion, being significantly different from the control.  
Based on the results obtained for cytotoxicity in 3T3 cells, a photoproduction assay using the concentrations closer to 100% of cell viability found for each extract was performed (Figure 2). Cells were exposed to a UV radiation dose capable of killing 50% of cells in the presence and absence of extracts. The results showed that none of the extracts tested presented a photoproduction capacity. From the six extracts, E1, E3 and E6 did not show any differences when compared with the control (cells without extracts and exposed to UV radiation), while E2 (\(p<0.05\)), E4 (\(p<0.0001\)) and E5 (\(p<0.001\)) revealed a phototoxic behavior.