## 4 Discussion  
### Nature of the Reaction Product  
It is well established that haem-peroxidases oxidize SCN\({}^{-}\) into OSCN\({}^{-}\) and I\({}^{-}\) into HOI/I\({}_{2}\) in the presence of H\({}_{2}\)O\({}_{2}\)[9; 10]. While studying biological lactoperoxidase activity in the presence of an SCN\({}^{-}\)/I\({}^{-}\) mixture, a few articles have addressed the products generated under such and such \begin{table}
\begin{tabular}{c c c c c} \hline
**[KI + KSCN] (mM)** & **KI/KSCN Ratio** & **[KI-KSCN-H\({}_{2}\)O\({}_{2}\)] (mM)** & **Dilution Factor** & _B. cinerea_ **Inhibition (\%)** \\ \hline \multicolumn{5}{c}{**Influence of the total [KI + KSCN] concentration**} \\ \hline \multirow{3}{*}{6.6 mM} & \multirow{3}{*}{4.5} & 5.4-1.2-6.6 & 1.3 & \(88\pm 5\) \\  & & 5.4-1.2-6.6 & 1.5 & \(84\pm 4\) \\  & & 5.4-1.2-6.6 & 1.10 & \(83\pm 2\) \\ \hline \multirow{3}{*}{4.4 mM} & \multirow{3}{*}{4.5} & 3.6-0.8-3.6 & 1.3 & \(88\pm 5\) \\  & & 3.6-0.8-3.6 & 1.5 & \(88\pm 6\) \\  & & 3.6-0.8-3.6 & 1.10 & \(70\pm 11\) \\ \hline \multirow{3}{*}{3.3 mM} & \multirow{3}{*}{4.5} & 2.7-0.6-3.3 & 1.3 & \(88\pm 6\) \\  & & 2.7-0.6-3.3 & 1.5 & \(83\pm 10\) \\  & & 2.7-0.6-3.3 & 1.10 & \(34\pm 1\) \\ \hline \multirow{3}{*}{5.6 mM} & \multirow{3}{*}{7.84} & 5-0.64-5.64 & 1.3 & \(92\pm 0\) \\  & & 5-0.64-5.64 & 1.5 & \(69\pm 11\) \\  & & 5-0.64-5.64 & 1.10 & \(42\pm 6\) \\ \hline \multirow{3}{*}{3.95 mM} & \multirow{3}{*}{7.84} & 3.5-0.45-3.95 & 1.3 & \(71\pm 11\) \\  & & 3.5-0.45-3.95 & 1.5 & \(42\pm 5\) \\  & & 3.5-0.45-3.95 & 1.10 & \(32\pm 8\) \\ \hline \multirow{3}{*}{2.25 mM} & \multirow{3}{*}{7.84} & 2-0.25-2.25 & 1.3 & \(38\pm 0\) \\  & & 2-0.25-2.25 & 1.5 & \(39\pm 6\) \\  & & 2-0.25-2.25 & 1.10 & \(29\pm 7\) \\ \hline \multicolumn{5}{c}{**Influence of the KI/KSCN ratio**} \\ \hline \multirow{3}{*}{1.12 mM} & \multirow{3}{*}{2.3} & 0.78-0.34-1.2 & 1.3 & \(90\pm 4\) \\  & & 0.78-0.34-1.2 & 1.5 & \(25\pm 0\) \\  & & 0.78-0.34-1.2 & 1.10 & \(22\pm 0\) \\ \hline \multirow{3}{*}{3.9 mM} & \multirow{3}{*}{2.25} & 2.7-1.2-3.9 & 1.3 & \(84\pm 1\) \\  & & 2.7-1.2-3.9 & 1.5 & \(81\pm 2\) \\  & & 2.7-1.2-3.9 & 1.10 & \(24\pm 7\) \\ \hline \multirow{3}{*}{2.95 mM} & \multirow{3}{*}{2.25} & 1.35-0.6-1.9 & 1.3 & \(87\pm 3\) \\  & & 1.35-0.6-1.9 & 1.5 & \(52\pm 4\) \\  & & 1.35-0.6-1.9 & 1.10 & \(20\pm 12\) \\ \hline \multirow{3}{*}{2.4 mM} & \multirow{3}{*}{1.1} & 1.35-1.2-2.5 & 1.3 & \(28\pm 4\) \\  & & 1.35-1.2-2.5 & 1.5 & \(31\pm 4\) \\  & & 1.35-1.2-2.5 & 1.10 & \(26\pm 5\) \\ \hline \multirow{3}{*}{1.3 mM} & \multirow{3}{*}{1.1} & 0.67-0.6-1.27 & 1.3 & \(16\pm 6\) \\  & & 0.67-0.6-1.27 & 1.5 & \(17\pm 6\) \\  & & 0.67-0.6-1.27 & 1.10 & \(13\pm 2\) \\ \hline \multicolumn{5}{c}{**Influence of the H\({}_{2}\)O\({}_{2}\) concentration**} \\ \hline \multirow{3}{*}{2.64 mM} & \multirow{3}{*}{4.5} & 2.16-0.48-0.5 & 1.3 & \(10\pm 7\) \\  & & 2.16-0.48-0.5 & 1.5 & \(12\pm 3\) \\  & & 2.16-0.48-0.5 & 1.10 & \(20\pm 2\) \\ \hline \multirow{3}{*}{2.64 mM} & \multirow{3}{*}{4.5} & 2.16-0.48-1.1 & 1.3 & \(39\pm 3\) \\  & & 2.16-0.48-1.1 & 1.5 & \(10\pm 9\) \\  & & 2.16-0.48-1.1 & 1.10 & \(7\pm 7\) \\ \hline \multirow{3}{*}{2.64 mM} & \multirow{3}{*}{4.5} & 2.16-0.48-2.7 & 1.3 & \(80\pm 3\) \\  & & 2.16-0.48-2.7 & 1.5 & \(45\pm 5\) \\  & & 2.16-0.48-2.7 & 1.10 & \(14\pm 1\) \\ \hline \end{tabular}
\end{table} 
Table 7: Total [KI + KSCN] dose (mM), KI/KSCN ratio, [KI + KSCN + H\({}_{2}\)O\({}_{2}\)] doses (mM), dilution factor, percentage of inhibition of _B. cinerea_ growth by various doses of (KI + KSCN +H\({}_{2}\)O\({}_{2}\)) after lactoperoxidase oxidoreductase. The doses were grouped to observe the influence of the total pseudohalogen and halogen concentrations, of the KI/KSCN ratio, and of the H\({}_{2}\)O\({}_{2}\) concentration. Means and s.d. of the two independent metals are given. experimental conditions [11; 12; 21; 27]; several have showed that a more stable oxidative complex was formed in specific pH and ionic strength conditions [25; 27; 28; 29].