## 2 Results and Discussion
### Gene Ontology Enrichment Analyses of PbPP1c-Interacting Proteins
\begin{table}
\begin{tabular}{l l c c c c c} \hline \hline
**GO ID** & **GO Term** & \
\begin{tabular}{c} **Number of** \\ **Genes Detected 1** \\ \end{tabular} & **Fold Enrichment** & \(p\)**-Value** & **Benjamini FDR** &
\begin{tabular}{c} **Bonferroni** \\ **Adjusted \(p\)-Value** \\ \end{tabular} \\ \hline GO:0022607 & Cellular component assembly & 20/108 & 2.6 & \(4.63\times 10^{-5}\) & \(2.55\times 10^{-4}\) & \(1.53\times 10^{-3}\) \\ GO:0065003 & Protein-containing complex assembly & 18/80 & 3.16 & \(6.93\times 10^{-6}\) & \(4.57\times 10^{-5}\) & \(2.29\times 10^{-4}\) \\ GO:0022618 & Ribonucleotide complex assembly & 13/43 & 4.25 & \(4.39\times 10^{-6}\) & \(3.62\times 10^{-5}\) & \(1.45\times 10^{-4}\) \\ \hline GO:0009058 & Biosynthesis possible process & 71/531 & 1.88 & \(4.53\times 10^{-9}\) & \(7.47\times 10^{-8}\) & \(1.49\times 10^{-7}\) \\ GO:0034641 & Cellular nitrogen compound & 87/756 & 1.62 & \(7.49\times 10^{-8}\) & \(8.24\times 10^{-7}\) & \(2.47\times 10^{-6}\) \\ GO:0006412 & Translation & 57/232 & 3.45 & \(4.86\times 10^{-9}\) & \(1.60\times 10^{-17}\) & \(1.60\times 10^{-17}\) \\ \hline \hline \end{tabular}  
* Number of genes detected/total number of genes in the background with this term.  
\end{table} 
Table 1: Analysis of biological processes enrichment in the PbPP1c interactome in schizons limited to GO SLIM terms. ([[https://plasmogem.shinyapps.io/schizont_interactions/](https://plasmogem.shinyapps.io/schizont_interactions/)]([https://plasmogem.shinyapps.io/schizont_interactions/](https://plasmogem.shinyapps.io/schizont_interactions/)) accessed on 15 September 2021) and GO enrichment of cellular process ([PERSON] adjusted \(p\)-value = 0.0001), including up to 12 proteins involved in RNA splicing ([PERSON] adjusted \(p\)-value = 0.009), supportive of our IP-MS of _P. berghei_ PP1c and the idea of its role in the regulation of the activity of spliceosomal factors. In our study, the \"cellular component assembly\" functional network also included five eukaryotic translation initiation factor 3 (elF3) subunits, of which four are members of the \"RNA transport\" KEGG pathway (Figure 3). One of the major nodes of this network was the elF3 subunit A (elF3a, PBANKA_1428700) with 11 edges that could be expected to contribute to forming the translation initiation complex. Of note, elF3a contains four potential RVxF binding motifs, suggesting a potential interaction with PP1c and a regulation of the complex formation by this phosphatase. From our GO Slim analysis, it could also be  
Figure 3: STRING network visualization of PbPP1c-interacting proteins belonging to “cellular component assembly” GO Slim in schizont stages. Using the Cytoscape STRING application, a network was retrieved for the 20 proteins from the cellular component assembly GO Slim of the PbPP1c interactome in schizont stages. With a protein–protein interaction enrichment \(p\)-value of 4.48 \(\times\) 10\({}^{-10}\), the resulting network contained 53 functional associations among 19 proteins; one protein (PBANKA_126600, tubulin gamma chain, putative) with no associations to other proteins in the network was removed. Proteins from the KEGG pathways map03050 (Proteasome), map03013 (RNA transport), and map03040 (Spliceosome) are colored in blue, green, and orange, respectively. Assignation to Eukaryotic translation initiation 3 subunits was conducted manually based on the protein description in PlasmaDB. Black stars indicate proteins with RVxF motifs based on the Wakula consensus sequence [19]. Proteins in each network are listed in adjacent tables showing the PlasmoDB geneID, short name, and protein description. STRING analysis was performed using the 11.0b version of the software. suggested that PP1c may contribute to the control of the translation process itself (Table 1) in _Plasmodium_. Indeed, 57 proteins belonging to this GO Slim pathway were significantly retrieved in the PbPP1c interactome. This includes the five eIF3 subunits already observed in the \"cellular component assembly\" network but also 49 ribosomal proteins.