## 3 Results  
### Results of In Vitro Testing of the Antioxidant Capacity  
#### 3.1.1 DPPH Method  
A calibration curve for Trolox and alpha-tocopherol was created to calculate the micrograms of the Trolox/alpha tocopherol equivalent per gram of sample (\(\mu\)g TE/g and \(\mu\)g AE/g) in the hydrophilic and lipophilic extracts of the samples tested. The calibration curve for Trolox was in the concentration range of 0.4-20 \(\mu\)g/mL, while the calibration curve for alpha-tocopherol was in the concentration range of 2-5 \(\mu\)g/mL. The absorbances for the hydrophilic and lipophilic extracts were measured for each sample and based on the data obtained (Tables 1 and 2), expressed as the percentage of inhibition and micrograms of Trolox equivalent per gram (\(\mu\)g TE/g) for the hydrophilic extracts and as the percentage of inhibition and micrograms of alpha tocopherol equivalent per gram(\(\mu\)g AE/g) for the lipophilic extracts (Tables 3 and 4).  
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**SAMPLE** & **A1** & **A2** & **A3** & \(\frac{-}{X}\) & **SD** & **CV (\%)** \\ \hline BLANK & 2.416 & 2.417 & 2.416 & 2.416 & 0.0007 & 0.03 \\
1 & 1.993 & 1.994 & 1.993 & 1.993 & 0.0007 & 0.04 \\
2 & 2.008 & 2.007 & 2.008 & 2.008 & 0.0010 & 0.05 \\
3 & 2.108 & 2.106 & 2.106 & 2.016 & 0.0010 & 0.05 \\
4 & 1.174 & 1.175 & 1.178 & 1.176 & 0.0026 & 0.22 \\
5 & 1.752 & 1.751 & 1.748 & 1.750 & 0.0021 & 0.12 \\
6 & 2.197 & 2.195 & 2.200 & 2.197 & 0.0025 & 0.11 \\
7 & 1.889 & 1.900 & 1.899 & 1.899 & 0.0007 & 0.04 \\ \hline \hline \end{tabular}
\end{table}
Table 1: Absorbances obtained for hydrophilic extracts of cream samples (DPPH method).  
\begin{table}
\begin{tabular}{c c c c c} \hline \hline
**SAMPLE** & **A1** & **A2** & **A3** & \(\frac{-}{X}\) & **SD** & **CV (\%)** \\ \hline BLANK & 1.924 & 1.923 & 1.923 & 1.923 & 0.0010 & 0.04 \\
1 & 1.002 & 1.003 & 1.002 & 1.002 & 0.0007 & 0.04 \\
2 & 1.616 & 1.616 & 1.615 & 1.616 & 0.0070 & 0.35 \\
3 & 1.482 & 1.481 & 1.484 & 1.483 & 0.0010 & 0.05 \\
4 & 0.323 & 0.323 & 0.324 & 0.323 & 0.0021 & 0.18 \\
5 & 1.553 & 1.553 & 1.554 & 1.553 & 0.0021 & 0.12 \\
6 & 1.551 & 1.554 & 1.553 & 1.552 & 0.0025 & 0.11 \\
7 & 0.368 & 0.368 & 0.367 & 0.368 & 0.0007 & 0.04 \\ \hline \hline \end{tabular}
\end{table}
Table 2: Absorbances obtained for lipophilic extracts of cream samples (DPPH method).  
\begin{table}
\begin{tabular}{c c c} \hline \hline
**Sample** & **\% of Inhibition** & **\(\mu\)g TE/g of Sample** \\ \hline
1 & 17.51 & 82.31 \\
2 & 16.89 & 79.88 \\
3 & 16.56 & 78.56 \\
4 & 51.32 & 215.69 \\
5 & 27.57 & 121.98 \\
6 & 9.06 & 49.00 \\
7 & 21.81 & 97.66 \\ \hline \hline \end{tabular}
\end{table}
Table 3: Results expressed as percentage of inhibition and in \(\mu\)g Trolox equivalent (TE)/g of sample (DPPH method).  
In the hydrophilic samples, the extract from sample 4 had the highest antioxidant capacity, followed by extracts 5 and 7. Slightly lower, but similar values were found in cream extracts 1, 2, and 3. The hydrophilic extract from cream 6 had the lowest antioxidant capacity. Among the lipophilic extracts, cream extracts 4 and 7 had the highest antioxidant capacity, followed by the lipophilic extract of cream 1. The antioxidant capacity values were similar among the lipophilic cream extracts 3, 6, and 5 while the extract of cream 2 had the lowest antioxidant capacity.