## 2 Results
### Genomic Data Evaluation  
ONT sequencing of the NBP-49626 genome generated 387,760 filtered long reads (7,926,827,031 bp), with a mean length of 20,442.61 bp and an N50 length of 29,025 bp. MGI sequencing produced 23,614,314 filtered short reads (3,542,147,100 bp), obtained from the 200-400 bp paired-end DNA library. As presented in Table 1, the consensus assembly revealed that the total length of the NBP-49626 genome was 37,366,436 bp, and it consists of eight chromosomes with an N50 length of 4,967,589 bp, resulting in 212.1-fold coverage on ONT and 94.8-fold coverage on MGI. The G + C content of the genome was 49.07%. A total of 10,131 protein-coding genes were predicted in the NBP-49626 genome. The average size of the protein-coding genes is 1663.62 bp, with an average of 3.24 exons per gene. Among the ncRNAs, 58 rRNAs (9, 10, and 39 copies of 18S, 28S, and 5S rRNAs, respectively), 164 tRNAs, and 36 sRNAs were identified. Based on public databases, 9991 of 10,131 genes (98.62%) were successfully annotated. Of these genes, 9983 (98.54%), 7676 (75.77%), 6025 (59.47%), 3514 (34.69%), 3347 (33.04%), 2771 (27.35%), 1435 (14.16%), and 177 (1.74%) were annotated using the NCBI NR, SWISS-PROT, GO, KEGG, KOG, PHI-base, CAZy, and Fungal cytochrome P450 databases, respectively. And 715 (7.06%) secreted protein genes were identified using a combination of signal peptide and transmembrane domain prediction (Table S1). In addition, the phylogenetic analysis based on the concatenated sequence alignments of the  
Figure 1: Determination of Okaramine B production of fungal strains in both submerged and semi-solid fermentations for three days. The level of significance was established at \(p<0.05\) (labeled as *), \(p<0.01\) (labeled as **), and \(p<0.001\) (labeled as ***). NS indicates no significant difference. molecular identification markers (including rDNA internal transcribed spacer (_ITS_), \(\beta\)-tubulin (_BenA_), calmodulin (_CaM_), and RNA polymerase II second-largest subunit regions (_RPB2_) of strain NBP-49626 and closely related _Penicillium_ type strains) indicates that it belongs to _Penicillium daleae_ (Figure S2). \begin{table}
\begin{tabular}{l l} \hline
**Genome Features** & **Value** \\ \hline Genome size (bp) & 37,366,436 \\ Number of chromosomes & 8 \\ N50 scaffold length (bp) & 4.967,589 \\ N90 scaffold length (bp) & 2,981,452 \\ Maximum scaffold length (bp) & 7,888,045 \\ Minimum scaffold length (bp) & 2,295,385 \\ G + C content (\%) & 49.07 \\ Complete BUSCOs of genome (\%) & 99.47 \\ Number of protein-coding genes & 10,131 \\ Avg gene length (bp) & 1663.62 \\ Avg CDS length (bp) & 1479.65 \\ Average number of exons per gene & 3.24 \\ Average exon length (bp) & 456.45 \\ Average intron length (bp) & 82.07 \\ rRNAs & 58 \\ tRNAs & 164 \\ Complete BUSCOs of genes (\%) & 99.08 \\ Number of annotated genes by NR & 9983 \\ Number of annotated genes by SWISS-PROT & 7676 \\ Number of annotated genes by GO & 6025 \\ Number of annotated genes by KEGG & 3514 \\ Number of annotated genes by KOG & 3347 \\ Number of annotated genes by PHI-base & 2771 \\ Number of annotated genes by CAZY & 1435 \\ Number of annotated genes by P450 & 177 \\ Number of secreted proteins & 715 \\ \hline \end{tabular} BUSCO, Benchmarking Universal Single-Copy Orthologs; CDS, coding DNA sequence.  
\end{table} 
Table 1: Chromosome-level genome features of _Penicillium daleae_ NBP-49626. Figure 2: Sequential determination of Okaramine B production of _Penicillium daleae_ NBP-49626 in both submerged and semi-solid fermentations. The level of significance was established at \(p<0.05\) (labeled as *), \(p<0.01\) (labeled as **), and \(p<0.001\) (labeled as **). NS indicates no significant difference. Using NCBI COG mapping, 3755 genes were assigned to COG categories.