## 3 Results and Discussion
### Simultaneous Determination of the 14 Analytes in Freeze-Dried SDGT Samples Using LC-MS/MS  
The 14 marker components in the SDGT extract were simultaneously analyzed and quantified by using the established LC-MS/MS MRM assay. The 14 analytes (LIQA, LIQ, NOD, NAR, NARG, HES, NPON, PON, API, GLY, HON, DEC, DECA, and PRAA) for simultaneous determination in the sample were detected at 4.65, 4.70, 5.03, 5.12, 5.27, 5.45, 6.58, 6.58, 7.15, 8.09, 9.74, 9.91, 9.96, and 10.14 min (Figure 2 and Figure S2), respectively, and the concentrations of these analytes were found to be 0.005-0.384 mg/g (Table 5).  
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & \multicolumn{5}{c}{**Batch No.**} \\ \cline{2-6}
**Compound** & \multicolumn{2}{c}{**1**} & \multicolumn{2}{c}{**2**} & \multicolumn{2}{c}{**3**} \\ \cline{2-6}  & **Mean (mg/g) \(\pm\) SD** & **Mean (mg/g) \(\pm\) SD** & **Mean (mg/g) \(\pm\) SD** & **Mean (\(\times\)10\({}^{-2}\))** & **RSD (\%)** \\  & **(\(\times\)10\({}^{-2}\))** & **RSD (\%)** & **Mean (\(\times\)10\({}^{-2}\))** & **RSD (\%)** & **Mean (\(\times\)10\({}^{-2}\))** & **RSD (\%)** \\ \hline LIQA & 2.07 \(\pm\) 2.77 & 1.34 & 2.08 \(\pm\) 5.30 & 2.54 & 2.05 \(\pm\) 1.49 & 0.73 \\ LIQ & 0.41 \(\pm\) 1.15 & 2.80 & 0.41 \(\pm\) 0.07 & 0.18 & 0.42 \(\pm\) 1.15 & 2.72 \\ NOD & 2.09 \(\pm\) 1.43 & 0.68 & 2.08 \(\pm\) 2.41 & 1.16 & 2.13 \(\pm\) 1.87 & 0.88 \\ NAR & 2.20 \(\pm\) 1.94 & 0.88 & 2.23 \(\pm\) 0.63 & 0.28 & 2.23 \(\pm\) 2.80 & 1.25 \\ NARG & 3.02 \(\pm\) 4.10 & 1.36 & 3.05 \(\pm\) 1.45 & 0.48 & 3.05 \(\pm\) 2.31 & 0.76 \\ HES & 3.82 \(\pm\) 8.20 & 2.14 & 3.82 \(\pm\) 3.09 & 0.81 & 3.86 \(\pm\) 1.70 & 0.44 \\ NPON & 0.55 \(\pm\) 0.57 & 1.05 & 0.56 \(\pm\) 1.17 & 2.10 & 0.56 \(\pm\) 0.24 & 0.43 \\ PON & 5.24 \(\pm\) 11.53 & 2.20 & 5.20 \(\pm\) 0.57 & 0.11 & 5.25 \(\pm\) 1.92 & 0.37 \\ API & 0.05 \(\pm\) 0.02 & 0.37 & 0.05 \(\pm\) 0.02 & 0.36 & 0.06 \(\pm\) 0.05 & 0.83 \\ GLY & 1.98 \(\pm\) 1.19 & 0.60 & 1.99 \(\pm\) 2.08 & 1.05 & 2.02 \(\pm\) 0.72 & 0.36 \\ HON & 0.05 \(\pm\) 0.04 & 0.77 & 0.05 \(\pm\) 0.08 & 1.58 & 0.05 \(\pm\) 0.07 & 1.34 \\ DEC & 0.66 \(\pm\) 0.11 & 0.17 & 0.53 \(\pm\) 0.06 & 0.11 & 0.66 \(\pm\) 0.08 & 0.12 \\ DECA & 0.53 \(\pm\) 0.14 & 0.27 & 0.66 \(\pm\) 0.17 & 0.26 & 0.53 \(\pm\) 0.06 & 0.11 \\ PRAA & 0.12 \(\pm\) 0.12 & 0.93 & 0.13 \(\pm\) 0.05 & 0.37 & 0.13 \(\pm\) 0.06 & 0.48 \\ \hline \hline \end{tabular}
\end{table}
Table 4: Amount of the 14 marker components in SDGT based on HPLC–PDA analysis (\(n=3\)).  
\begin{table}
\begin{tabular}{c c c c} \hline \hline  & \multicolumn{3}{c}{**Amount (mg/g)**} \\ \cline{2-4}
**Compound** & **Mean** & **SD (\(\times\)10\({}^{-2}\))** & **RSD (\%)** \\ \hline LIQA & 0.171 & 0.930 & 5.448 \\ LIQ & 0.037 & 0.029 & 0.785 \\ NOD & 0.309 & 2.034 & 6.592 \\ NAR & 0.156 & 1.161 & 7.428 \\ NARG & 0.246 & 2.382 & 9.691 \\ HES & 0.384 & 1.910 & 4.971 \\ NPON+PON & 0.046 & 0.223 & 4.841 \\ API & 0.005 & 0.002 & 0.495 \\ GLY & 0.206 & 1.188 & 5.763 \\ HON & 0.005 & 0.013 & 2.289 \\ DEC & 0.138 & 0.943 & 6.837 \\ DECA & 0.142 & 0.938 & 6.588 \\ PRAA & 0.029 & 0.064 & 2.208 \\ \hline \hline \end{tabular}
\end{table}
Table 5: Amount of the 14 marker components in SDGT based on LC–MS/MS analysis (\(n=3\)).