## 3 Results  
### Phenolic Compounds and Antioxidant Activity of Formulated Aggregates  
The total phenolic, proanthocyanidin and monomeric anthocyanin contents, along with the antioxidant activities of the formulated aggregates, are presented in Table 1. Comparing parameters that were evaluated on formulated aggregates, it can be concluded that the amount of protein matrix used for complexation highly affected the adsorption of polyphenols and consequently affected antioxidant activity. Aggregates formulated with 2% of the protein matrix had higher values of all evaluated parameters than aggregates with 6% of the protein matrix. Even though the increase in protein matrix during complexation was threefold, the decrease of adsorption didn't follow that trend. Total phenolic contents of aggregates were 874.93 mg/100 g and 369.78 mg/100 g for Cas2%_TC and Cas6%_TC, respectively. Aggregates with 2% of protein matrix had a concentration of proanthocyanidin's 619.45 mg/100 g and aggregates with 6% of protein matrixhad a concentration of 273.18 mg/100 g. In both cases, the concentration was 2.3 times higher in Cas2%_TC than in Cas6%_TC. A lower decrease with an increase of protein amount was observed for monomeric anthocyanins, from 137.52 mg/100 g to 78.75 mg/100 g, indicatingthat Cas2%_TC had 1.7 times higher concentration than Cas6%_TC. Additionally, values of antioxidant activities that were determined by the DPPH, ABTS, CUPRAC and FRAP methods also did not follow a trend of threefold increase. For DPPH antioxidant activity, Cas2%_TC had values 1.2 times higher than those of Cas6%_TC, while for all other methods, the antioxidant activity of Cas2%_TC was around 2 times higher than that of Cas6%_TC. These results indicate that methods are based on different mechanisms of action but also that individual phenolic compounds contribute differently to antioxidant activity. Specific phenolic compounds were also evaluated in formulated aggregates and are presented in Table 2 (additionally Figures S1-S4). Two anthocyanins were identified, cyanidin-3-glucosyl-rutinoside and cyanidin-3-rutinoside. As it was determined for monomeric anthocyanins, both individual anthocyanins were determined in higher concentration in aggregates with the lower amount (2%) of the protein matrix, 504.66 mg/kg vs. 783.09 mg/kg for aggregates with 6% of protein matrix. These aggregates had 1.1 times higher concentrations of these anthocyanins than aggregates with 6% of protein matrix (458.72 mg/kg and 675.5 mg/kg, respectively). Two flavonoids were identified, rutin and quercetin, and their adsorption was also affected by the amount of protein matrix. The same pattern as for anthocyanins was determined. Cas2%_TC had 1.2 times higher concentration of both flavonoids than Cas6%_TC. Rutin was evaluated in concentrations of 259.79 mg/kg and 209.02 mg/kg in Cas2%_TC and Cas6%_TC, respectively, while quercetin for the same samples was evaluated in lower concentrations, 89.57 mg/kg and 79.33 mg/kg, respectively. Chlorogenic acid, neochlorogenic acid and \(p\)-coumaric acid and its derivate were identified from phenolic acids. All of these acids were determined in higher concentrations in Cas2%_TC: 470.23 mg/kg, 352.90 mg/kg, 559.40 mg/kg and 927.04 mg/kg, respectively. In this aggregate, these concentrations were 2.3 higher for chlorogenic and neochlorogenic acids and 1.3 times higher for \(p\)-coumaric acid and its derivate than in Cas6%_TC (186.44 mg/kg, 159.88 mg/kg, 454.07 mg/kg and 626.51 mg/kg, respectively). Additionally, epicatechin was identified in aggregates in a concentration of 1156.63 mg/kg in Cas2%_TC, which was 4.4 times higher than in Cas6%_TC (249.26 mg/kg). Formulated aggregates differ in their color parameters, which are presented in Table 3. L* value (lightness) of the protein matrix decreased with the adsorption of tart cherry juice components onto it. Cas6%_TC aggregate had a significantly higher L* value than was expected since that sample contained a higher amount of protein matrix.