## 3 Results and Discussion
### Antimicrobial Activity  
The shelf life of edible oils and their applicability in industry are greatly dependent on their oxidative stability and antioxidant and antibacterial activity [40]. In our study, the best activity of flax seed oil (Table 3) was determined against _Bacillus cereus_ CCM 2010 (4.58 mm), followed by _Salmonella enterica_ subs. _ enterica_ CCM 3807 (3.07 mm). Increased flax seed oil proportions in the human diet can reduce immune function markers. The improvement of acute pneumonia was reported to be mediated by enhancing the intake of flax seed oil-based polyunsaturated fatty acids [4]. The effect of flax seed oil supplementation on the course of pneumonia caused by _Streptococcus pneumoniae_ was studied in a mouse model [41]. Long-term supplementation safeguarded animals against lung colonization with _S. pneumoniae_, with a decrease in histopathological involvement of lung tissue. Supplemented infected mice showed moderate pneumonia compared to severe pneumonia in unsupplemented mice [41]. [PERSON] et al. [42] showed that methicillin-resistant _Staphylococcus aureus_ (MRSA), methicillin-sensitive _Staphylococcus aureus_ (MSSA), _Klebsiella pneumoniae_, and _Staphylococcus epidermidis_ were all affected in various ways. The application of flax seed oil also aided in the healing of experimental wounds. In their research, the most potent antibacterial activity of poppy seed oil was observed against Haemophilus influenzae CCM 4454, Escherichia coli CCM 3954, _Klebsiella pneumoniae_ CCM 2318, and _Streptococcus pneumoniae_ CCM 4501. \begin{table} \begin{tabular}{c c c c c c} \hline \hline FAME [\%] & FO & PO & MTO & GO & WO \\ \hline C16:0 & 5.56 \(\pm\) 0.03 e & 8.99 \(\pm\) 0.03 a & 7.93 \(\pm\) 0.02 b & 7.46 \(\pm\) 0.02 c & 6.64 \(\pm\) 0.29 d \\ C18:0 & 3.85 \(\pm\) 0.03 b & 2.16 \(\pm\) 0.08 d & 5.13 \(\pm\) 0.02 a & 3.85 \(\pm\) 0.02 b & 2.56 \(\pm\) 0.02 c \\ C18:1n9c & 19.69 \(\pm\) 0.02 b & 15.46 \(\pm\) 0.13 e & 30.34 \(\pm\) 0.25 e & 17.39 \(\pm\) 0.75 d & 18.26 \(\pm\) 0.19 e \\ C18:1n9t & 0.73 \(\pm\) 0.04 c & 1.11 \(\pm\) 0.02 a & 0.62 \(\pm\) 0.01 d & 0.73 \(\pm\) 0.02 c & 0.84 \(\pm\) 0.02 b \\ C18:2n6f & - & - & - & 0.62 \(\pm\) 0.03 a & - & - \\ C18:2n6c & 17.92 \(\pm\) 0.67 e & 68.31 \(\pm\) 0.26 b & 47.41 \(\pm\) 0.25 d & 69.25 \(\pm\) 0.16 a & 60.06 \(\pm\) 0.42 c \\ C18:3n3c & 51.64 \(\pm\) 0.49 a & 3.19 \(\pm\) 0.08 c & 1.47 \(\pm\) 0.05 d & - & 10.50 \(\pm\) 0.17 b \\ C20:0 & - & - & 0.54 \(\pm\) 0.01 a & - & - \\ C20:1n9c & - & - & 0.62 \(\pm\) 0.04 a & - & - \\ C22:0 & - & - & 2.97 \(\pm\) 0.02 a & - & - \\ \hline \end{tabular}   
FOOTNOTE:a]Footnote a: footnotemark:Flux seeds demonstrated different levels of inhibitory and antibiofilm effects on a range of bacterial strains, such as MRSA, _S. epidermidis_, _K. pneumoniae_, and MSSA. The application of flax seed oil showed positive effects on wound healing in experiments [42]. The most potent antibacterial activity observed in our study was seen in poppy seed oil, particularly against _Bacillus cereus_ CCM 2010 (4.32 mm), _Escherichia coli_ CCM 3954 (0.31 mm), _Klebsiella pneumoniae_ CCM 2318 (3.68 mm), and _Streptococcus pneumoniae_ CCM 4501 (0.14 mm). This oil had the best overall antimicrobial activity. Our results are comparable with the results of [PERSON] and [PERSON] [43], who determined an inhibition zone for _Staphylococcus aureus_ of 2.00 mm, but the activity of this oil against _Escherichia coli_ and _Bacillus cereus_ was stronger in our study (inhibition zone diameters of 0.31 mm and 4.32 mm, respectively). In the case of the referenced study, these zones were smaller (0.10 for both). Moreover, these authors determined antifungal activity of poppy seed oil towards _Aspergillus niger_ (MTCC 281) and _Aspergillus oryzae_ (MTCC 624)--3 mm--and for _Penicillium chrysogenum_ (MTCC 6795)--2 mm. The studied milk thistle seed oil exhibited the strongest activity against _Clostidium perfringens_ CCM 4991 (6.31 mm) (Table 3). In a study by [PERSON] et al.