## 5 Conclusions  
In this work, we evaluated the impact of WA-related edge effects, defined by the COI correction, on two types of LD microcirculatory signals routinely evaluated in assessing microcirculatory dynamics: baseline signals and transient signals modelled by challenging PORH. This is the first study to investigate the importance of the COI in biological signals evaluating microcirculation. Our results suggest that the COI correction has little effect in assessing baseline signals. However, applying the COI correction is crucial for transient signals whose rapid fluctuations often characterize physiological short-lived events. Failure to do so can lead to inaccurate WA results and, consequently, conclusions about the effects of physiological influences on overall microcirculatory control. Therefore, we believe that our findings are important for researchers and clinicians working with LD microcirculatory data and that the results have implications for improving the accuracy of physiological assessments. Conceptualization, H.L., L.K. and M.H. ; methodology, H.L., L.K. and M.H. ; software, L.K. and M.H. ; formal analysis, L.K. ; investigation, H.L., L.K. and M.H. ; data curation, H.L. and L.K. ; writing--original draft preparation, L.K. ; writing--review and editing, H.L., L.K. and M.H. ; visualization, L.K. ; supervision, H.L. All authors have read and agreed to the published version of the manuscript. This article was part of the project funded by the Slovenian Research Agency, funding no. P3-0019. This study was conducted in accordance with the Declaration of Helsinki and approved by the National Medical Ethics Committee of the Republic of Slovenia (No. 0120-175/2017/6, Date of approval: 23 September 2020). Informed Consent Statement: Informed consent was obtained from all subjects involved in this study Data Availability Statement: The representative data and the basic code for the implemented algorithm are available in the public repository ([[https://github.com/[PERSON]/Flowmotion](https://github.com/[PERSON]/Flowmotion)]([https://github.com/[PERSON]/Flowmotion](https://github.com/[PERSON]/Flowmotion))), accessed on 20 November 2024. Further requests regarding the datasets should be directed to [EMAIL_ADDRESS]. We would like to thank all the volunteers who participated in this study. Our gratitude also goes to [PERSON] for her assistance with figure editing. The authors declare no conflicts of interest. References  
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