## 2 Materials and Methods  
### Materials  
Selected proteases included alkaline protease (5.0 \(\times\) 10\({}^{5}\) U/g), weakly alkaline protease (4.8 \(\times\) 10\({}^{5}\) U/g), neutral protease (1.5 \(\times\) 10\({}^{5}\) U/g), acid protease (8.0 \(\times\) 10\({}^{5}\) U/g), trypsin (4.0 \(\times\) 10\({}^{3}\) U/g), aminopeptidase (6.0 \(\times\) 10\({}^{4}\) U/g), flavourzyme (5.0 \(\times\) 10\({}^{5}\) U/g), and keratinase (1.0 \(\times\) 10\({}^{5}\) U/g) in food-grade solid powder form from Lonct Enzymes Co., Ltd. (Linyi, China). Protease activity is expressed as protease activity units. At 40 \({}^{\circ}\)C and a certain pH, the amount of enzyme required for protease to hydrolyze casein to produce 1 ug of tyrosine per minute is one unit of enzyme activity. The pH of the reaction conditions for measuring the enzymatic activity of alkaline proteases, weakly basic proteases, aminopeptides, and keratinases was 10.5. The pH of the reaction conditions for measuring the enzymatic activity of neutral proteases, trypsin, and flavored proteases was 7.5. The pH of the reaction conditions for measuring the enzymatic activity of acidic proteases was 3.0.  
Detergent builders included polyethoxylated fatty alcohols, sodium ethoxy alkyl sulfate, dodecylbenzesulfonic acid, triethanolamine, anhydrous sodium citrate, SNS-80, each in industrial-grade (\(\geq\)90%) from Research Institute of Daily Chemical Industry (Taiyuan, China). 2-morpholineethanesulfonic acid (MES) in molecular biology grade (>99%) from Coolaber. Fatty acid methyl ester ethoxylate (FMEE), alkyl glycoside (APG), layered sodium disilicate (SKS-6) from Yousuo Chemical Technology Co., Ltd. (Linyi, China), modified oil ethoxylate (SOE) from Junxin Chemical Technology Co., Ltd. (Guangzhou, China), tea saponin from Zhongye Biotechnology Co., Ltd. (Lishui, China), sodium alginate, hyaluronic acid from Boxbio Science & Technology Co., Ltd. (Beijing, China), all of the above are industrial grade (\(\geq\)90%). Sodium carboxymethyl cellulose (CMC), polyvinyl alcohol type 1799 (PVA), polyethylene glycol 6000 (PEG), polyaspartic acid (PASP), silicon dioxide (SiO\({}_{2}\)), ethylenediaminetetraacetic acid tetrasodium salt (EDTAA), disodium maleate were used in chemically pure forms (\(\geq\)99.5%) from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Hydroxypropyl methylcellulose sodium (HPMC), hydroxyethyl cellulose sodium, and sodium polyacrylate were used in food-grade (>99%) from Best Food Additives Co., Ltd. (Zhengzhou, China). 4A zeolite was industrial-grade (\(\geq\)90%) from Runfeng Synthetic Technology Co., Ltd. (Nantong, China). Sodium tartrate and sodium gluconate in food-grade (>99%) were acquired from Gukang Biological Engineering Co., Ltd. (Jinan, China). Sodium laurate in industrial-grade (\(\geq\)90%) was from Longhui Chemical Co., Ltd. (Jinan, China). \(\alpha\)-cyclodextrintrin, \(\beta\)-cyclodextrintrin, and \(\gamma\)-cyclodextrintrin were used in food-grade (>99%) from Youlezi Food Ingredients Co., Ltd. (Shanghai, China). Sulfobutyl-\(\beta\)-cyclodextrintrin (Captisol), methyl-\(\beta\)-cyclodextrintrin, and 2-hydroxypropyl-\(\beta\)-cyclodextrintrin were analytical reagents (\(\geq\)99.9%) from Aladdin (Shanghai, China). In the experiment, the materials used to simulate protein stains were eggs and carbon powder. Blood used was porcine anticoagulated whole blood. Double distilled water was used in the experiments.