## 3 Results and Discussion
### Human Pathogenic Microorganisms
\begin{table}
\begin{tabular}{p{113.8 pt} p{113.8 pt} p{113.8 pt} p{113.8 pt}} \hline
**Microorganism** & **Observations** & **HRTS1310.1 \({}^{\text{a}}\)** & **HRTS1310.2 \({}^{\text{b}}\)** & **HRTS1310.3 \({}^{\text{c}}\)** \\ \hline \multirow{6}{*}{_Mycobacterium leprae_} & _Mycobacterium leprae_ causes leprosy ([PERSON]’s disease). This disease can leave its signature in bones if untreated. It primarily leads to the destruction of peripheral nerves and muscles and their atrophy, and the motor function is impaired. The loss of sensation in the extremities causes accidental injuries with subsequent infections. In addition, loss of bone calcium contributes to a slow shrinking of the digits and their transition to claw/pencil-like forms in late-stage leprosy [10,48,70]. & 0.005 & 0.033 & 0.043 \\ \hline \multirow{6}{*}{_Mycobacterium gilvum_} & This is an environmental mycobacterium isolated from river sediments. _M. gilvum_ has rarely been isolated as an opportunistic pathogen [69] and isuspended as a nontuberculous mycobacterium and as nonpathogenic and found in water and humans [71]. Its presence has been detected in accumulated water associated with a bathing place for leprosy patients [72]. & 0.011 & 0.148 & 0.290 \\ \hline \multirow{6}{*}{_Trichomonas vaginalis_} & May cause vaginitis or sexually transmitted infections [48,73]. It is a commensal in the gut, oral cavity, and female genital tract and can be found in soil, sewage, feces, and marine sedimentations. It was once considered to be nonpathogenic; however, it has been recently associated with septic arthritis, osteomyelitisitis, soft tissue infection, brain abscess, bacteremia, and endocarditis cases [74,75]. & 0.011 & 0.148 & 0.290 \\ \hline \multirow{6}{*}{_Staphylococcus aureus_} & _Staphylococcus aureus_ is a highly virulent, life-threatening pathogen that can cause diseases such as deep abscess, osteomyelitisitis, meningitis, sepsis, endocarditis, or pneumonia (to name but a few examples) [62,66]. & 0.055 & 0.032 & 0.007 \\ \hline \multirow{6}{*}{_Treponema_ sp. This pathogen evolves from environmental organisms and a human parasite [47,48]. The most common interrogans_} & 0.055 & 0.032 & 0.007 \\ \cline{1-1}  & & The genus _Treponema_ includes a wide diversity of commensal and pathogen species, most of which exist in complex bacterial communities [47,48]. & 0.328 & 0 \\ \hline \multirow{6}{*}{_Leptospira interrogans_} & This pathogen evolves from environmental organisms and a human parasite [47,48]. The most common leptopspiral syndrome in humans is _leptospirosis_, a disorder that localizes in the kidneys and can cause renal failure and ultimately death [48]. & 0.049 & 0.580 \\ \cline{1-1}  & The various colors correspond to the percentage gradient: darker colors = higher percentages. The values represent the ratio of the analyses shown as the number of specific readings divided by the number of total readings. The readings have been debugged to remove errors/artifacts using the MG-RAST server. The reads obtained per sample.* 24.942 spots, 15M bases (tooth sample); * 33.035 spots, 19.9M bases (tooth sample); * 89.422 spots, 53.8M bases (bone sample). & 0.049 & 0.580 \\ \cline{1-1}  & The various colors correspond to the percentage gradient: darker colors = higher percentages. The values represent the ratio of the analyses shown as the number of specific readings divided by the number of total readings. The readings have been debugged to remove errors/artifacts using the MG-RAST server. The reads obtained per sample.* 24.942 spots, 15M bases (tooth sample); * 33.035 spots, 19.9M bases (tooth sample); * 89.422 spots, 53.8M bases (bone sample). & 0.049 & 0.580 \\ \hline \end{tabular}
\end{table} 
Table 1: List of the most abundant human pathogenic microorganisms found in the various samples of the HRTS SK1310 individual and associated frequency. soft tissue infection and meningitis can leave marks on bone, since they compromise the macro- and microstructure of the cortical, compact, and cancellous bone tissue [10].