# Differentiation in Aquatic Metabolism between
## 3 Results  
### Spatiotemporal Dynamics of Environmental Parameters  
The results showed the expected seasonal trends in monthly means of the abiotic parameters calculated by pooled daily measurements (all sites included). The variation of dissolved oxygenconcentration and pH followed a clear seasonal pattern in both years (Figure 2). Higher monthly means of DO were recorded in winter (January 2016, January 2017 and February 2018) followed by a declining trend until November (2016, 2017). The peak of mean monthly pH was observed in September, for both 2016 and 2017 (8.82 and 8.66 respectively), and the minimum in November (7.87 in 2016 and 8.03 in 2017). Conductivity also fluctuated seasonally showing an obvious decline until September followed by a recovery until early spring. Chl-a concentration showed a less obvious seasonal variation with peaks recorded in January, September, and October of 2016 and in May of 2017. By further examining the seasonal trends for each monitoring site we identify the same consistent pattern, but in parallel we can observe the presence of small spatial differences (Figure 3).  
Figure 3: Monthly trends in chlorophyll-a concentration (Chl-a), electrical conductivity (EC), dissolved oxygen concentration (DO) and pH per site.  
Figure 2: Monthly means (pooled samples) of chlorophyll-a concentration (Chl-a), electrical conductivity (EC), dissolved oxygen concentration (DO) and pH. Error bars indicate standard error (SE).  
By comparing the investigated parameters among the four habitats we found statistically significant differences, highlighted by the Kruskal-Wallis test (Figure 4). S2 site presented the highest mean pH, DO concentration and Chl-a concentration and the lowest conductivity (8.42, 8.66 mg L\({}^{-1}\), 12.46 \(\upmu\)g L\({}^{-1}\) and 293.29 \(\upmu\)S cm\({}^{-1}\) respectively). The highest conductivity and the lowest pH means were observed in site S4 (339.28 \(\upmu\)S cm\({}^{-1}\) and 8.22 respectively), while S3 was characterized by the lowest mean DO and Chl-a concentrations (6.67 mg L\({}^{-1}\) and 4.94 \(\upmu\)g L\({}^{-1}\)). Temporal trends were identical for all sites except for a significant increase of conductivity in site S4 that occurred from June 2017 until February 2018. In addition, site S2 exhibited a remarkable increase of Chl-a concentration in April and May of 2017 compared with the other sites.  
Concerning the meteorological parameters the variability showed the expected seasonality (Figure 5). It is worth noting the highly variable air temperature ranging from several degrees below 0 \({}^{\circ}\)C in winter up to 30 \({}^{\circ}\)C during the summer. Wind speed in general remained low with events of higher value (but no more than 4 m/s) occurring more frequently during winter.  
Figure 4: Means of chlorophyll-a concentration (Chl-a), dissolved oxygen concentration (DO), electrical conductivity (EC) and pH among the four monitoring sites/littoral habitats. Error bars indicate standard errors (SE).  
Figure 5: Daily variation of meteorological variables (from top to bottom: Wind speed, PAR, relative humidity, air temperature).