## 4 Discussion
& Ricotta Second CW & \multirow{2}{*}{10.77} & \multirow{2}{*}{6.8} & \multirow{2}{*}{0.63} & \multirow{2}{*}{0.15} & \multirow{2}{*}{0.094} & [26] \\  & & & C/N adjustment: & & & & & & \\ \cline{1-1} \cline{5-10}  & \multicolumn{2}{c}{} & \multirow{2}{*}{(NH\({}_{4}\))\({}_{2}\)SO\({}_{4}\)} & \multirow{2}{*}{38.50} & \multirow{2}{*}{1.4} & \multirow{2}{*}{0.04} & \multirow{2}{*}{0.19} & \multirow{2}{*}{0.006} & [25] \\ \cline{1-1} \cline{5-10}  & \multicolumn{2}{c}{} & \multirow{2}{*}{(NH\({}_{4}\))\({}_{2}\)SO\({}_{4}\)} & \multirow{2}{*}{33.60} & \multirow{2}{*}{6.7} & \multirow{2}{*}{0.31} & \multirow{2}{*}{0.16} & \multirow{2}{*}{0.030} & \multirow{2}{*}{[37]} \\ \cline{1-1} \cline{5-10}  & \multicolumn{2}{c}{} & \multirow{2}{*}{(NH\({}_{4}\))\({}_{2}\)SO\({}_{4}\)} & \multirow{2}{*}{66.80} & \multirow{2}{*}{33.1} & \multirow{2}{*}{0.50} & \multirow{2}{*}{0.67} & \multirow{2}{*}{0.490} & \multirow{2}{*}{[35]} \\ \cline{1-1} \cline{5-10}  & \multicolumn{2}{c}{} & \multirow{2}{*}{(NH\({}_{4}\))\({}_{2}\)SO\({}_{4}\)} & \multirow{2}{*}{66.80} & \multirow{2}{*}{33.1} & \multirow{2}{*}{0.50} & \multirow{2}{*}{0.67} & \multirow{2}{*}{0.490} & \multirow{2}{*}{[35]} \\ \cline{1-1} \cline{5-10}  & \multicolumn{2}{c}{} & \multirow{2}{*}{(NH\({}_{4}\))\({}_{2}\)SO\({}_{4}\)} & \multirow{2}{*}{6.80} & \multirow{2}{*}{33.20} & \multirow{2}{*}{0.16} & \multirow{2}{*}{0.09} & \multirow{2}{*}{0.015} & \multirow{2}{*}{[35]} \\ \cline{1-1} \cline{5-10}  & \multicolumn{2}{c}{} & \multirow{2}{*}{(NH\({}_{4}\))\({}_{2}\)SO\({}_{4}\)} & \multirow{2}{*}{6.80} & \multirow{2}{*}{0.09} & \multirow{2}{*}{0.015} & \multirow{2}{*}{0.09} & \multirow{2}{*}{0.015} & \multirow{2}{*}{[35]} \\ \cline{1-1} \cline{5-10}  & \multicolumn{2}{c}{} & \multirow{2}{*}{(NH\({}_{4}\))\({}_{2}\)SO\({}_{4}\)} & \multirow{2}{*}{6.80} & \multirow{2}{*}{0.09} & \multirow{2}{*}{0.015} & \multirow{2}{*}{0.09} & \multirow{2}{*}{0.015} & \multirow{2}{*}{[36]} \\ \cline{1-1} \cline{5-10}  & \multicolumn{2}{c}{} & \multirow{2}{*}{(NH\({}_{4}\))\({}_{2}\)SO\({}_{4}\)} & \multirow{2}{*}{6.80} & \multirow{2}{*}{0.09} & \multirow{2}{*}{0.015} & \multirow{2}{*}{0.09} & \multirow{2}{*}{0.015} & \multirow{2}{*}{[37]} \\ \cline{1-1} \cline{5-10}  & \multicolumn{2}{c}{} & \multirow{2}{*}{(NH\({}_{4}\))\({}_{2}\)SO\({}_{4}\)} & \multirow{2}{*}{6.80} & \multirow{2}{*}{33.20} & \multirow{2}{*}{0.16} & \multirow{2}{*}{0.09} & \multirow{2}{*}{0.006} & \multirow{2}{*}{[25]} \\ \cline{1-1} \cline{5-10}  & \multicolumn{2}{c}{} & \multirow{2}{*}{(NH\({}_{4}\))\({}_{2}\)SO\({}_{4}\)} & \multirow{2}{*}{6.80} & \multirow{2}{*}{0.09} & \multirow{2}{*}{0.06} & \multirow{2}{*}{[35]} \\ \cline{1-1} \cline{5-10}  & \multicolumn{2}{c}{} & \multirow{2}{*}{(NH\({}_{4}\))\({}_{2}\)SO\({}_{4}\)} & \multirow{2}{*}{6.80} & \multirow{2}{*}{33.1} & \multirow{2}  
Despite the restricted lipid accumulation process that was observed during growth of yeasts on second CW (K\({}_{\text{L/X}}\) < 16% \(w\)/\(w\); see Table 2), triacylglycerleverols were the major lipid compound identified for almost all strains tested. This is in agreement with results reported by other oleaginous and non-oleaginous microorganisms (i.e., _Yarrowia lolytica_, _Cunningham echinulata_, _Thumidium elegans_, etc. ), which, irrespective of the imposed culture conditions and the subsequent low or high lipid accumulation observed, produced cellular lipids that were in most cases rich in neutral fractions and mostly triacylglycerleverols [58; 59; 60]. On the other hand, in agreement with the literature [3; 33; 34; 55], the principal FAs found in variable quantities inside the studied yeast lipids were mainly oleate and palmitate and to lesser extent linoleate and stearate (Table 5), being therefore potential candidates for the synthesis of second generation biodiesel, oleochemicals, and biolubricants [33; 59].