## 3 Results and Discussion
### Antibacterial Activity  
The in vitro antibacterial activity of the four sprout extracts analyzed on the five bacterial strains tested had been evaluated qualitatively and quantitatively by the presence or absence of inhibition zones, percentages of bacterial inhibition, and the resulting MIC values. Figure 6 shows images of Petri dishes in which different doses of extracts were applied to the four bacterial strains analyzed, and Table 3 shows the diameters (in mm) of \begin{table}
\begin{tabular}{c c c c c c c c c c} \hline \hline
**Samples** & & & & & & & & & & \\ \hline  & **Cu** & **Ni** & **Zn** & **Fe** & **Mn** & **Ca** & **Mg** & **K** & **Na** \\ \hline  & & & & & & Composite & & & & \\ \hline WF & 3.530 \(\pm\) 0.27 \({}^{\ast}\) & nd \({}^{\ast}\) & 14.37 \(\pm\) 0.20 \({}^{\ast}\) & 11.781 \(\pm\) 0.11 \({}^{\ast}\) & 2.050 \(\pm\)0.04 \({}^{\ast}\) & 141.510 \(\pm\) 0.64 \({}^{\ast}\) & 401.160 \(\pm\) & 499.320 \(\pm\) & 191.320 \(\pm\) \\  & & & & & & & & & \\  & & & & & & & & & \\  & & & & & & & & & \\  & & & & & & & & & \\  & & & & & & & & & & \\ \hline  & & & & & & & & & & \\  & & & & & & & & & & \\ \end{tabular}  
WF—wheat flour, PS—pea sprouts, RS—rabdish sprouts, BS—beam sprouts, LS—lentil sprouts. The values are expressed as mean values \(\pm\) standard deviations of all measurements; data within the same column sharing different superscripts are significantly different (\(p<0.05\)); data within the same column sharing the same superscripts are not significantly different (\(p>0.05\)). \({}^{\ast}\)nd—detectable.  
\end{table} 
Table 2: The macro- and microelements composition of sprouts and wheat flour. the inhibition zones under the action of the various volumes of extracts that were analyzed. Figure 6: The antimicrobial effect of BS, LS, PS, RS against _E. coli, P. mirabilis, K. pneumoniae, and _E. faecalis_. It should be emphasized that the diameters of the inhibition zone for each positive control and, respectively, for each tested extract, included the 6 mm diameter of the micro-tablet. For the _Escherichia coli_ strain, isolated from an infected wound, the effectiveness of the antibiotic used (microtablet containing 5 ug of levofloxacin) generated an inhibition zone (halo) of 33 mm. The tested extracts had no effect against _E. coli_, the colonies developing up to the proximity of the microtablets soaked with 2, 50, 70, and 100 \(\mu\)L of the extract. For _Proteus mirabilis_, it was proven to be sensitive to gentamicin, which generated an inhibition zone of 25 mm. On the other hand, the inefficiency of the four analyzed extracts was proven, regardless of the concentration applied. Regarding the _K. pneumoniae_ strain, the microtablet containing 5 ug of ciprofloxacin, which was considered a positive control, generated a halo of 17 mm. The tested extracts were without effect for this strain at a volume of 2 \(\mu\)L of extract. It was also checked for antibacterial activity of the extracts at larger volumes of 50 \(\mu\)L, 70 \(\mu\)L, and 100 \(\mu\)L were without any bactericidal action. Compared to the other strains studied, _E. fecalis_ has been shown to be sensitive to radial sprout extract in concentrations of 50 \(\mu\)L, 70 \(\mu\)L, and 100 \(\mu\)L, with an inhibition diameter between 12 and 14 mm; also, the inhibition zone was observed lowering RS concentration (2 \(\mu\)L). The microtablet containing 5 ug of vancomycin, considered a positive control, generated a halo of 16 mm; the rest of the analyzed extracts were without any inhibitory effect for this strain. For _S. aureus_, the microtablet with 10 ug of linez solid, considered a positive control, generated a halo of 33 mm. Two of the tested extracts, BS and LS, had no effect on the tested strain at volumes of 2 \(\mu\)L of extract, but the PS generated an 11 mm halo, while the microtablet, softened with 2 \(\mu\)L extract from RS, generated a 13 mm halo. For increased volumes of extracts microtablets, an antibacterial effect against _S.