# Plant In Vitro Cultures of _Coleus scutellarioides_ (L.[PERSON] [PERSON].
## 2 Results  
### Plant In Vitro Cultures  
The effective induction of cultures was conducted using exclusively stems with nodes (plants without roots/shoots or leaves). The stems themselves showed vitality and produced roots. However, shoot production was not observed. The plants cultivated under in vitro conditions responded differently to the composition of the breeding medium (Figure 1a-f). The stems of plants bred on media supplemented with 0.5 mg dm\({}^{-3}\) of BA and 0.5 mg dm\({}^{-3}\) of 2 iP turned black at the place of immediate contact with the substratum (Figure 1b,c). Additionally, the root growth cones in the plants cultivated on the medium enriched with 0.5 mg dm\({}^{-3}\) of fluridone were observed to turn brown, or they assumed the white color (Figure 1d). The best rooting (96.7% on average) was recorded for the medium supplemented with auxins. Under control conditions, the rooting process was lower by over 8.8% than that observed for the medium enriched with auxin IAA. Cytokinesind added to the breeding medium reduced the rooting of explants by 67.7%, whereas the addition of fluridone caused a decline in explants rooting by 19.5%, as compared with the control. The shoot production was found to be the same under each circumstances--all the plants produced shoots. The highest survivability, at a level of 95.8%, was characteristic of those explants which were cultivated on the medium supplemented with auxins. Cytokinesind the lowering of survivability by over 11.6% in comparison with the control conditions, while fluridone had the worst effect on survivability, reducing it by over 19.5% compared with that of the control. The variance analysis performed has shown that the type of phytohormones used affects all of the analyzed traits, except for the number of shoots. The largest mean root length--7.04 cm--was attained on the medium with an addition of IAA and on that enriched with NAA. Supplementation with fluridone resulted in a decrease in the root  
Figure 1: _C. scutellarioules_ “Electric Lime” cultivated in vitro under different conditions. (**a**)—MS control medium; (**b**)—MS + 0.5 mg dm\({}^{-3}\) BA; (**c**)—MS + 0.5 mg dm\({}^{-3}\) 2 iP; (**d**)—MS + 0.5 mg dm\({}^{-3}\) fluridone; (**e**)—MS + 0.5 mg dm\({}^{-3}\) NAA; (**f**)—MS + 0.5 mg dm\({}^{-3}\) IAA. M. Jakobina. length by 2 cm compared to plants cultivated on the control medium. The roots were over three times shorter on the breeding medium with BA, and over five times shorter on the medium supplemented with 2 iP in comparison with the plants cultivated under control conditions, i.e., without plant growth regulators. The best growth of shoots was observed in the plants bred on the medium enriched with auxins (IAA or NAA) and those bred on the control medium, with shoots over 2 cm long. Fluidone added to the breeding medium caused a decrease in the shoot length by 1.4 times as compared with that of the plants cultivated on the medium lacking phytohormones, whereas the addition of cytokins (BA or 2 iP) resulted in a double reduction of the shoot length as compared with that of the control sample (Figure 2a). The highest mean number of roots was attained in the case of explants bred on the medium enriched with IAA, with a ca 1.5 times more than that noted on the control medium. More than nine times fewer roots were produced on the media supplemented with cytokins in comparison with the rate under control conditions. The average number of roots on the medium enriched with fluiridone equaled 6.22. For the number of shoots--the level of which was 2.75-4.57 cm (Figure 2b)--no statistically significant differences were noted. Figure 2: Influence of cultivation conditions on the growth of _C. scutellaroides_ “Electric Lime” explants (**a**) influence of supplementation with plant growth regulators on the root length and shoot length; (**b**) influence of addition of phytohormones on the number of roots and shoots. Values followed by a different letter are significantly different (\(p>0.05\), according to the Tukey test). ns—not significant.