# The Effect of Hydrolysis on the Antioxidant Activity of Olive Mill Waste
## 3 Results
### Correlation Analysis
#### 3.3.2 Correlation between TPC, TFC and TdOPC with Metal Ion Reducing Assays (FRAP and CUPRAC)  
Figure 10 shows the correlation between TPC, TFC and TdOPC with FRAP and CUPRAC. It was found that TPC significantly positively correlated with both FRAP and CUPRAC in all extraction procedures (FRAP: alkaline-\(\rho\) = 0.76, acid-\(\rho\) = 0.80, maceration-\(\rho\) = 0.78 and \begin{table}
\begin{tabular}{c c c c c} \hline \hline \multicolumn{5}{c}{**Superoxide Radical Scavenging Activity**} \\ \hline
**Cultivar** & **Date** & \begin{tabular}{c} _IC\$50 of Alkaline Hydrolysis_ \\ \end{tabular} & \begin{tabular}{c} _IC\$50 of Acid_ \\ _Hydrolysis_ \\ \end{tabular} & \
\begin{tabular}{c} _IC\$50 of Acid_ \\ _Hydrolysis_ \\ \end{tabular} &
\begin{tabular}{c} _IC\$50 of_ \\ _Maceration_ \\ _Extraction_ \\ \end{tabular} \\ \hline _Bidni_ & 26 October 2021 & 18.38 \(\pm\) 1.38 & 36.68 \(\pm\) 2.12 & 44.79 \(\pm\) 9.10 \\ _Bidni_ & 2 November 2021 & 12.70 \(\pm\) 0.24 & 16.68 \(\pm\) 2.05 & 8.07 \(\pm\) 5.36 \\ _Bidni_ & 16 November 2021 & 4.43 \(\pm\) 3.97 & 11.40 \(\pm\) 3.82 & 11.23 \(\pm\) 10.15 \\ _Bidni_ & 29 September 2021 & 5.32 \(\pm\) 0.41 & 8.37 \(\pm\) 3.48 & 2.46 \(\pm\) 1.77 \\ BDS & 28 September 2021 & 1.30 \(\pm\) 0.09 & 12.18 \(\pm\) 4.58 & 8.96 \(\pm\) 1.30 \\ Carolea & 7 October 2021 & 11.25 \(\pm\) 0.12 & 3.36 \(\pm\) 0.92 & 13.27 \(\pm\) 5.03 \\ Carolea & 22 October 2021 & 3.39 \(\pm\) 2.54 & 5.06 \(\pm\) 1.40 & 11.69 \(\pm\) 2.08 \\ Carolea & 17 November 2021 & 0.28 \(\pm\) 0.35 & 9.48 \(\pm\) 1.59 & 13.27 \(\pm\) 4.77 \\ Carolea & 19 October 2021 & 0.85 \(\pm\) 0.38 & 9.00 \(\pm\) 1.50 & 10.62 \(\pm\) 1.02 \\ Cerasuola & 2 November 2021 & 15.66 \(\pm\) 2.29 & 9.07 \(\pm\) 4.79 & 7.91 \(\pm\) 0.83 \\ Coratina & 27 September 2021 & 5.57 \(\pm\) 4.09 & 11.98 \(\pm\) 3.12 & 8.44 \(\pm\) 9.72 \\ Coratina & 25 October 2021 & 7.60 \(\pm\) 1.62 & 8.86 \(\pm\) 2.66 & 23.53 \(\pm\) 4.69 \\ Franttoio & 27 September 2021 & 14.08 \(\pm\) 5.85 & 10.02 \(\pm\) 3.32 & 17.59 \(\pm\) 4.47 \\ Franttoio & 4 October 2021 & 1.30 \(\pm\) 0.95 & 4.32 \(\pm\) 0.51 & 28.00 \(\pm\) 2.42 \\ Franttoio & 19 October 2021 & 4.00 \(\pm\) 0.59 & 9.59 \(\pm\) 1.25 & 3.28 \(\pm\) 3.37 \\ Leccino & 17 October 2021 & 16.10 \(\pm\) 10.41 & 5.10 \(\pm\) 0.64 & 6.86 \(\pm\) 5.34 \\ _Malti_ & 29 September 2021 & 13.87 \(\pm\) 2.71 & 7.11 \(\pm\) 0.81 & 13.01 \(\pm\) 10.37 \\ Mixed 1 & 2 October 2021 & 23.86 \(\pm\) 4.26 & 5.66 \(\pm\) 0.94 & 12.58 \(\pm\) 3.40 \\ Mixed 2 & 30 September 2021 & 20.95 \(\pm\) 6.29 & 27.51 \(\pm\) 6.28 & 8.55 \(\pm\) 4.38 \\ _Bajda 1_ & 4 October 2021 & 14.86 \(\pm\) 3.42 & 8.70 \(\pm\) 2.97 & 3.77 \(\pm\) 2.39 \\ _Bajda 2_ & 4 October 2021 & 10.40 \(\pm\) 2.94 & 7.19 \(\pm\) 2.00 & 18.90 \(\pm\) 4.50 \\ Picholine & 7 October 2021 & 1.83 \(\pm\) 1.19 & 7.04 \(\pm\) 1.48 & 22.26 \(\pm\) 15.31 \\ UDP & 4 October 2021 & 4.94 \(\pm\) 0.46 & 3.63 \(\pm\) 0.46 & 2.90 \(\pm\) 2.19 \\ UDP & 7 October 2021 & 14.61 \(\pm\) 0.49 & 5.36 \(\pm\) 1.50 & 11.03 \(\pm\) 0.79 \\ UDP & 25 October 2021 & 9.22 \(\pm\) 2.08 & 10.87 \(\pm\) 0.41 & 20.74 \(\pm\) 1.18 \\ \hline \hline \end{tabular}
\end{table} 
Table 3: IC\({}_{508}\) of olive oil extracts for _Superoxide Radical Scavenging_ activity assays. (BDS—Bella di Spagnaand UDP—Uovo di Piccione). CUPRAC: alkaline-\(\rho\) = 0.93, acid-\(\rho\) = 0.57, maceration-\(\rho\) = 0.69). In the case of TFC and TdOPC, it was found that macerated and alkaline hydrolys extracts showed a significant positive correlation with FRAP (TFC: macerated-\(\rho\) = 0.45, alkaline-\(\rho\) = 0.22 and TdOPC: macerated-\(\rho\) = 0.46, alkaline-\(\rho\) = 0.25), whereas for acid hydrolysis, there was no significant difference observed. However, CUPRAC significantly positively correlated with TFC and TdOPC in all extraction procedures (TFC: alkaline-\(\rho\) = 0.63, acid-\(\rho\) = 0.48, maceration-\(\rho\) = 0.84 and TdOPC: alkaline-\(\rho\) = 0.69, acid-\(\rho\) = 0.58 and macerated-\(\rho\) = 0.83). Figure 10: (**A**) Correlation between TPC and FRAP.