# Heat Stress Affects H3K9 me3 Level at Human Alpha Satellite DNA Repeats
## Results
### Dynamics of H3K9 me3 at Alpha Satellite Repeats after HS
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Alpha Repeat No** & \begin{tabular}{c} **The Increase of** \\ **/Number of** \\ **Minorms /** **Similarity to** \\ **Consensus \%** \\ \end{tabular} & \begin{tabular}{c} **The Gene** \\ **/** **H3K9 me3 (\(\times\))** \\ **after 2 h HS and** \\ **\(p\) Values** \\ \end{tabular} & \begin{tabular}{c} **Alpha** \\ **Repeat-** \\ **Associated** \\ **Gene** \\ \end{tabular} & \begin{tabular}{c} **Cell Line** \\ **to Gene and** \\ **Distance (bp)** \\ **of Alpha** \\ **Distance (bp)** \\ \end{tabular} & \
\begin{tabular}{c} **Distance of** \\ **Alpha** \\ **Repeat from** \\ **TSS (bp)** \\ \end{tabular} &
\begin{tabular}{c} **The Gene** \\ **Expression** \\ **downregulation** \\ **(o) after 1/2 h** \\ **HS and** \(p\) Values** \\ \end{tabular} \\ \hline
290 & 1.0 (0.211) & _ZNF675_ & Cal 27 & / & / & -71.0 (0.059) \\ \hline
290 & 1.0 (0.162) & _ZNF675_ & OV-90 & / & / & -71.0 (0.087) \\ \hline
290/0.6 / 88\% & 1.9 (0.012) & _ZNF675_ & MJ90 nTERt & intron & 25,978 & -71.7 (0.002) \\ \hline
290/0.6 / 88\% & 2.1 (0.008) & _ZNF675_ & HepG2 & intron & 25,978 & -71.7 (0.003) \\ \hline
10/1.1 / 86\% & 2.0 (0.011) & _NR3C1_ & MJ90 nTERt & intron & 125,971 & -73.5 (0.002) \\ \hline
10/1.1 / 86\% & 3.2 (0.014) & _NR3C1+allele_ & HepG2 & intron & 125,971 & -72.7 (0.004) \\ \hline
100 & 1.0 (0.158) & _NR3C1+allele_ & HepG2 & / & / & -71.0 (0.061) \\ \hline
10/1.1 / 86\% & 2.1 (0.012) & _NR3C1_ & Cal 27 & intron & 125,971 & -73.7 (0.007) \\ \hline
10/1.1 / 86\% & 1.7 (0.017) & _NR3C1_ & OV-90 & intron & 125,971 & -72.7 (0.012) \\ \hline
28/1.2 / 70\% & 3.7 (0.006) & _WAV1_ & MJ90 nTERt & intron & 25,978 & / \\ \hline
28/1.2 / 70\% & - & _WAV1_ & 697 & intron & 46,492 & 2.6- (0.001) \\ \hline
10/7 / 82\% & 4.1 (0.009) & _SLC30A6_ & MJ90 nTERt & intron & 48,094 & -72.0 (0.003) \\ \hline
14/1.7 / 70\% & 2.5 (0.011) & _PRIM2_ & MJ90 nTERt & intron & 59,161 & -72.8 (0.015) \\ \hline
18/0.5 / 76\% & 4.3 (0.012) & _STAM_ & MJ90 nTERt & intron & 9163 & -72.0 (0.004) \\ \hline
19/2.4 / 74\% & 3.6 (0.006) & _PLA2G12B_ & MJ90 nTERt & intron & 15,104 & / \\ \hline
25/1.2 / 88\% & 3.9 (0.018) & _MYO1E_ & MJ90 nTERt & intron & 152,899 & -72.3 (0.005) \\ \hline
21/0.7 / 85\% & 4.0 (0.005) & _DLG2_ & MJ90 nTERt & intron & 197,351 & / \\ \hline
31/1.3 / 71\% & 2.8 (0.011) & _MAP7_ & MJ90 nTERt & intron & 15,483 & / \\ \hline
3/1.4 / 77\% & 2.1 (0.012) & _SLC40A1_ & MJ90 nTERt & \(5^{\prime}\) / 38,888 & 38,888 & -72.0 (0.003) \\ \hline
3/1.4 / 77\% & - & _ASNSD1_ & MJ90 nTERt & \(5^{\prime}\) / 46,190 & 46,190 & -72.3 (0.014) \\ \hline
4/1.4 / 70\% & 1.9 (0.014) & _ST6 GAL1_ & MJ90 nTERt & \(3^{\prime}\) / 29,520 & 174,981 & -72.1 (0.001) \\ \hline
5/3.4 / 75\% & 1.8 (0.012) & _HTRA3_ & MJ90 nTERt & \(3^{\prime}\) / 34,942 & 72,296 & -75.6 (0.011) \\ \hline
5/3.4 / 75\% & - & _ACOX3_ & MJ90 nTERt & \(3^{\prime}\) / 24,328 & 98,092 & -71.7 (0.009) \\ \hline
15/2.0 / 86\% & 2.0 (0.011) & _INTS1_ & MJ90 nTERt & \(5^{\prime}\) / 14,580 & 14,850 & -72.0 (0.013) \\ \hline
16/0.5 / 72\% & 2.3 (0.018) & _PHF20L1_ & MJ90 nTERt & \(3^{\prime}\) / 15,772 & 89,221 & -72.9 (0.010) \\ \hline
17/0.5 / 94\% & 4.1 (0.011) & _DIP2C_ & MJ90 nTERt & \(5^{\prime}\) / 18,158 & 18,158 & -71.8 (0.010) \\ \hline
20/1.0 / 86\% & 2.4 (0.014) & OR6A2 & MJ90 nTERt & \(5^{\prime}\) / 14,531 & 14,531 & / \\ \hline
30/1.0 / 70\% & 1.7 (0.012) & _PPPP2R3B_ & MJ90 nTERt & \(5^{\prime}\) / 16,060 & 16,060 & / \\ \hline \hline \end{tabular}
\end{table} 
Table 1: The increase of H3K9 me3 level at dispersed alpha repeats immediately after heat stress as well as the level of downregulation of alpha repeat-associated genes in different human cell lines. Alpha repeat No, number of monomers within repeat, and similarity to alpha satellite consensus sequence is indicated as well as positions and distance of alpha repeats relative to genes and their distance from TSS. The \(p\)-values are calculated using the unpaired \(t\)-test. (- not determined;/ not present).