# Effects of Pulsed Electric Field on Oil Extraction Rate and
## Appendix A. Tocopherol Detection in Peony Seed Oil
The same sample solution was taken and placed at room temperature, and the peak areas of \(\alpha\)-tocopherol, \(\beta\)-tocopherol and \(\delta\)-tocopherol were injected at 0, 2, 4, 6, 8, and 24 h, respectively. The peak areas were recorded, and the RSD values of \(\alpha\)-tocopherol, \(\beta\)-tocopherol and \(\delta\)-tocopherol were calculated, respectively. The results are shown in Table A3. The RSD% of the 7 indexes was less than 3.0%, indicating that the solution was stable within 24 h after preparation and had no significant effect on the determination results. * [7] Repeatability test. The test samples were prepared according to the above-mentioned preparation method, and 6 samples were taken in parallel to prepare the test product solution with a certain concentration. According to the chromatographic conditions described in (4), the peak areas of \(\alpha\)-tocopherol, \(\beta\)/\(\gamma\)-tocopherol and \(\delta\)-tocopherol were, respectively, injected to determine the peak areas of \(\alpha\)-tocopherol, \(\beta\)-tocopherol and \(\delta\)-tocopherol, which were recorded, and the RSD values of the peak areas of \(\alpha\)-tocopherol, \(\beta\)-tocopherol and \(\delta\)-tocopherol were calculated, respectively. The results are shown in Table A4. The RSD of \(\alpha\)-tocopherol, \(\beta\)-tocopherol and \(\delta\)-tocopherol were 1.97%, 0.67% and 0.35%, respectively, and RSD% of the peak area of the three components was less than 3.0%, indicating good reproducibility of the method. \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Number of Times** & \(\alpha\)**-Tocopherol/A** & \(\beta\)**-Tocopherol/A** & \(\delta\)**-Tocopherol/A** \\ \hline
1 st & 736 & 613 & 805 \\
2 nd & 728 & 602 & 813 \\
3 rd & 738 & 616 & 814 \\
4 th & 739 & 634 & 818 \\
5 th & 748 & 624 & 812 \\
6 th & 716 & 602 & 804 \\ RSD (\%) & 1.49 & 2.03 & 0.67 \\ \hline \hline \end{tabular}
\end{table} 
Table A2: Precision test results of two reference substances. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Tocopherol** & **Linear Equation** & \(R\) & \begin{tabular}{c} **Linearity** \\ **Range/\(\mu\)g\(\cdot\)mL\({}^{-1}\)** \\ \end{tabular} & \
\begin{tabular}{c} **LOD (Limit of** \\ **Detection)/\(\mu\)g** \\ \end{tabular} &
\begin{tabular}{c} **LOQ (Limit of** \\ **Quantitiation)/\(\mu\)g** \\ \end{tabular} \\ \hline \(\alpha\) & y = 6102.3\(x-2.8202\) & \(R\) = 0.9999 & 2.65–212 & 0.18 & 0.54 \\ \(\beta\)/\(\gamma\) & y = 11,459\(x-0.625\) & \(R\) = 0.9997 & 6.25–100 & 0.24 & 0.72 \\ \(\delta\) & y = 33,365\(x-6.0833\) & \(R\) = 0.9999 & 6.25–100 & 0.27 & 0.82 \\ \hline \hline \end{tabular}
\end{table} 
Table A1: Result of the linear relationship inspection, detection limit and quantification limit determination (\(n=3\)). (9) Sample content determination. The appropriate amount of peony seed oil (about 0.1 g) of the above-mentioned prepared samples was moved into a graduated centrifuge tube. The samples were extracted for 30 min by using ultrasonic extraction (power 250 W, frequency 40 kHz) in where methanol as the extraction agent, and the materials-liquid ratio of 1:1.2 (g/mL). The above extraction process was proceeded twice. The extraction liquid after traction twice were combined and concentrated with nitrogen blowing apparatus, and then fixed volume with methanol to 0.15 mL. The samples were filtered as a sample liquid to be tested. The sample liquid was absorbed to be measured according to the chromatographic conditions described in step (3). The peak area of the target peak was recorded, and the content of \(\alpha\)-tocopherol, \(\beta\)-tocopherol and \(\delta\)-tocopherol in the sample were calculated.