## 3 Results
### Genomic Characterization of SM2 Isolate  
The sequence read data of the SM2 isolate were submitted to the PATRIC Comprehensive Genome Analysis Service. According to the complete analysis, the SM2 isolate was identified as _Pectobacterium aroidearum_ SM2. The genome quality assessment showed coarse consistency (98.5), fine consistency (97.6), CheckM completeness (100), and CheckM contamination (0.3), suggesting that this genome is of good quality. The genome contains 86 contigs with an estimated length of 5,037,920 bp and an average guanine-cytosine content of 51.46%. According to the data provided by RASTtk, the genome has 4925 protein coding sequences (CDSs), 69 transfer RNA (rRNA) genes, and four ribosomal RNA (rRNA) genes. Additionally, the genome of _P. aroidearum_ SM2 shows similarities to other known genes (Table 2), such as the virulence factors (Victors, VFDB, PATRIC), antibiotic resistance genes (PATRIC, CARD), transporters (TCDB), and drug target genes (DrugBank) that were  
Figure 1: Evaluation of the infective capacity of SM2 isolate. Fruits and tubers were inoculated with bacterial suspension at a concentration of 10\({}^{8}\) CFU/mL. (**A**) Jalapeno pepper (_Capsicum annuum_); (**B**) tomato (_Solanum lycopersicum_); (**C**) serrano chili (_Capsicum annuum_); (**D**) green tomato (_Physalis ioxcapa_); (**E**) potato (_Solanum luberosum_). Over 48 h, the soft rot symptoms were registered. identified in different databases. In addition, the genome annotation of the proteins with subsystem functional assignments is shown in Figure 2. The distribution of the subsystems by the category of _P. aroidearum_ SM2 is shown in Figure 3.  
Figure 2: Circular genomic map and subsystems information for _P. aroidearum_ SM2. From the outside to the center are the ensemble contigs, with CDSs in the front strand, ORF on the reverse strand, RNA genes, CDSs with similarity to known antibiotic resistance genes, CDSs with similarity to virulence factors, GC content, and GC skew. The distributions of the subsystems are displayed in the figure below. In terms of subsystems coverage, 45% indicates a total of 2269 genes and 55% represents those not indicated in the subsystem average, with a total of 2829 genes. According to the analysis of the genome of _P. aroidearum_ SM2, the predicted antibiotic resistance genes were grouped into six different mechanisms of action, as depicted in Figure 4. Antibiotic targets in susceptible species were represented by the following genes: _dxr_, _folA_, _dfr_, _rho_, _murA_, _gyrB_, _s10p_, _s12p_, _iso-tRNA_, _folP_, _eF-tu_, _ddl_, _gyrA_, _rpoC_, _altr_, _eF-G_, _inhA_, _fabl_, _kasA_, and _rpoB_. Regarding the mechanism of efflux pump conferring antibiotics resistance, the genes detected were _mdtABC-ToL_, _macA_, _acrAB-ToL_, _macB_, _acrAD-ToL_, _emrAB-ToL_, _acrAB-ToL_, _acrAB-ToL_, _acrZ_, and _tolC/opmH_. Regarding proteins altering the cell wall charge and conferring antibiotic resistance, only two genes were found: _psgA_ and _gdpD_. Regulators modulating the expression of antibiotic resistance were represented by _acrAB-ToL_, _h-NS_, _oxyR_, and _emrAB-ToL_. The antibiotic activation enzyme and the gene conferring resistance via absence were represented by a single gene each: _katG_ and _gidB_, respectively.