## 4 Discussion  
Elicitation is a natural method for the enhancement in plant resistance to biotic and abiotic stresses and improvement in the quality of edible plants, especially in relation to their health-promoting phytochemicals [15]. As indicated in many studies, jasmonic acid used as an elicitor can influence the biosynthesis of some plant secondary metabolites [28; 29]. It is well known that some plant metabolites, e.g., phenolic compounds or essential oil compounds, possess many biological activities; therefore, increased biosynthesis of the secondary metabolites has an effect on the biological activity of plants [18; 29]. Our previous publication indicated that elicitation with 0.01 mM jasmonic acid (JA1), 1 mM jasmonic acid (JA2), and 100 mM jasmonic acid (JA3) influenced the composition of essential oils from basil and increased the antioxidant and anti-inflammatory potential of these EOs (especially the JA2 and JA3 sample) [22]. Another very important activity attributed to essential oils is the ability to inhibit the acetylcholine transferase enzyme. Acetylcholinesteraseterase (AChE) is a key enzyme involved in the hydrolysis of acetylcholine in the nervous system of animals and insects. Acetylcholine deficiency in the human cerebral cortex is associated with neurodegenerative disorders, such as Alzheimer's \begin{table}
\begin{tabular}{l c c} \hline \hline \multicolumn{1}{c}{**Sample**} & _E. coli_ **ATCC 25922** & _S. aureus_ **ATCC 29737** \\ \hline \hline \multicolumn{1}{c}{Inhibition zone (mm) *} \\ \hline \hline C & 17.70 a \(\pm\) 2.65 & 15.70 a \(\pm\) 0.37 \\ \hline JA1 & 21.20 a,b \(\pm\) 1.79 & 23.60 b,e \(\pm\) 1.91 \\ \hline JA2 & 22.20 b \(\pm\) 3.34 & 19.00 a,c \(\pm\) 2.16 \\ \hline JA3 & 14.50 c \(\pm\) 1.12 & 18.00 a,c,d \(\pm\) 1.30 \\ \hline AMP & 13.90 c \(\pm\) 0.29 & 13.00 a,d \(\pm\) 0.44 \\ \hline NE & 22.90 b \(\pm\) 0.45 & 26.40 e \(\pm\) 0.98 \\ \hline OT & 15.70 b \(\pm\) 0.43 & 16.50 a \(\pm\) 0.22 \\ \hline E & 10.00 c,d \(\pm\) 0.63 & 17.00 a \(\pm\) 0.31 \\ \hline \multicolumn{1}{c}{MIC (\(\mu\)L mL-1)} \\ \hline \hline C & 0.625 & 0.625 \\ \hline JA1 & 0.625 & 0.625 \\ \hline JA2 & 0.625 & 0.625 \\ \hline JA3 & 0.312 & 0.156 \\ \hline \multicolumn{1}{c}{MBC (\(\mu\)L mL-1)} \\ \hline C & 1.250 & 0.625 \\ \hline JA1 & 0.625 & 0.625 \\ \hline JA2 & 1.250 & 0.625 \\ \hline JA3 & 0.625 & 0.312 \\ \hline \hline \end{tabular}  
*-values are expressed as mean \(\pm\) SD; different letters in the same column indicate significant differences (\(p<0.05\)); AMP-ampicillin (10 ug/disk); NE–neomycin (30 ug/disk); OX–oxytetracycline (30 ug/disk); E–erythromycin (15 ug/disk); MIC–minimum inhibitory concentration; MBC–minimum bactericidal concentrations; C, control; JA1, 0.01 mM jasmonic acid; JA2, 1 mM jasmonic acid; JA3, 100 mM jasmonic acid.  
\end{table} 
Table 3: Effect of jasmonic acid elicitation on antimicrobial activity of essential oil from basil. disease (AD). Nowadays, commercial drugs acting as selective acetylcholinesteraseinesterase inhibitors are used in the treatment of Alzheimer's disease. Yet, synthetic compounds exhibit some toxicity during prolonged use; hence, many researchers are looking for compounds of natural origin that can be a potential alternative for the treatment of AD patients. There are some reports on the ability of essential oils from some plants to inhibit acetylcholinesteraseinesterase activity, but there is no information about the effect of plant elicitation on these properties [7; 30]. Literature data prove that EOs from medicinal plants, especially from _Melissa officinalis_, _Citrus anantifolia_, _Ocinum gratisinsumum_, _Citrus anantitium_, _Cupressus semipervirens_, _Eucalyptus globulus_, _Foeniculum vulgare_, _Thymus vulgaris_, _Eucalyptus canaldulensis_, and _Ocinum canum_, have high acetylcholinesteraseinesterase inhibitory activity [7; 30].