# Phytocomplex Influences Antimicrobial and Health Properties of Concentrated Glycerine Macerates
## 2 Results
### GC-MS Analysis  
With IM-SPME/GC-MS, 7 volatile constituents and 3 three fatty acids were identified, which are listed in Table 2. Only in RN-A and RN-B samples the following components were found, at the respective contents: terpinolene (8.5%, 15.8%), terpinen-4-ol (33.1%, 33.5%), \(\alpha\)-methylbenzenepropanamine (13.2%, 11.6%), spathulenol (15.3%, 15.9%) and Caryophyllene oxide (29.8%, 31.1%). Octanoic acid was found only in FC-B sample (9.9%), while dodecanal only in AG-B sample (3.3%). FC-A and FC-B, CB-B and AG-B samples were rich in fatty acids. In particular, palmitic acid was the main component in FC-A (64.8%) followed by linoleic acid (22.8%) and linoleic acid (12.3%). On the contrary, in FC-B, the quantities of the 3 fatty acids were distributed more evenly (31.7%, 29.3%, and 29.0%, respectively). In the respective CB-B and AG-B samples, palmitic acid (20.5% and 31.9%), linoleic acid (28.0% and 29.4%), and linoleic acid (51.4% and 35.4%) were retrieved, while no compound was detected in both CB-A and AG-A samples. In conclusion, the findings clearly show that B-C-GMs were more concentrated with respect to A-C-GMs.  
Figure 1: Superposition of quercetin (brown) and myricetin (red) signals with those of FC-B (**a**) and FC-A (**b**) samples.  
\begin{table}
\begin{tabular}{c c c c c c c c c c c c c} \hline \hline
**No. \({}^{\text{a}}\)** & **COMPONENT \({}^{\text{b}}\)** & **LRI \({}^{\text{c}}\)** & **LRI\({}^{\text{b}\text{d}}\)** & **MS \({}^{\text{e}}\)** & **FC-A** & **FC-B** & **CB-A** & **CB-B** & **AG-A** & **AG-B** & **RN-A** & **RN-B** \\ \hline
1 & terpinolene & 1073 & 1079 \({}^{f}\) & + & - & - & - & - & - & - & 8.5 & 15.8 \\ \hline
2 & terpinen-4-ol & 1165 & 1168 & + & - & - & - & - & - & - & 33.1 & 33.5 \\ \hline
3 & octanoic acid & 1168 & 1170 & + & - & 9.9 & - & - & - & - & - & - \\ \hline
4 & \(\alpha\)-methylbenzenepropanamine & 1118 & 1221 & + & - & - & - & - & - & 13.2 & 11.6 \\ \hline
5 & dodecanal & 1384 & 1388 & + & - & - & - & - & - & 3.3 & - & - \\ \hline
6 & spathuelon & 1576 & 1581 & + & - & - & - & - & - & 15.3 & 15.9 \\ \hline
7 & caryophylene oxide & 1580 & 1585 & + & - & - & - & - & - & 29.8 & 31.1 \\ \hline
8 & palmitic acid (C16.0) & 1945 & 1950 & + & 64.8 & 31.7 & - & 20.5 & - & 31.9 & - & - \\ \hline
9 & linoleic acid (C18.2) & 2128 & 2130 & + & 22.8 & 29.3 & - & 28.0 & - & 29.4 & - & - \\ \hline
10 & linolenic acid (C18.3) & 2155 & 2159 & + & 12.3 & 29.0 & - & 51.4 & - & 35.4 & - & - \\ \hline \multicolumn{1}{c}{Total identified (\%)} & \multicolumn{1}{c}{99.9} & \multicolumn{1}{c}{99.0} & \multicolumn{1}{c}{99.9} & \multicolumn{1}{c}{100.0} & \multicolumn{1}{c}{99.9} & \multicolumn{1}{c}{100.0} \\ \hline \hline \end{tabular} Note. * indicates the compound identification number. \({}^{\text{b}}\) the components are reported according to their elution order on column; \({}^{\text{c}}\) Linear Retention Indices measured on polar volume; \({}^{\text{d}}\) Linear Retention Indices from the literature, identification by MS spectra; \(<\) tr \(<\) 0.1%* Mass spectrum; \(<\) Companion with mass spectra reported in NIST library. \({}^{\text{f}}\) Normal alkane RI.  
\end{table}
Table 2: Volatile chemical components (%) of C-CMs.