## 4 Discussion
In the EO:C 1:2 ratio, \(\alpha\)-bisabolol was also identified after 7 days of storage (2.1% and 2.5%, respectively), while chamazulene was identified only on the day of preparing the inserts (0.7%). For the third ratio of 1:3, \(\alpha\)-bisabolol appeared in the gas phase in all storage time intervals (1.7%, 1.8%, 2.1%, 2.5%, and 16%, respectively), while chamazulene only appeared on the day the inserts were prepared (1.6%). In the gas phase of the matrix with microcrystalline cellulose in a 1:1 ratio to the EO, \(\alpha\)-bisabolol was detected on the day of its preparation and after 7 and 14 days of storage, in amounts of 2.0%, 2.4%, and 1.5%, respectively. In the case of a matrix with an EO:MC ratio of 1:2, this alcohol was determined in the samples on the day of their preparation (1.9%) and after 7 (1.5%), 14 (2.1%), and 56 days (47.4%) of storage. For the EO:MC in the concentration of 1:3, \(\alpha\)-bisabolol was identified on the day of preparation of the matrices (2.3%) and after 14 days of storage (19.1%). Chamazulene was identified for EO:MC in concentrations of 1:1 and 1:2 in the matrix only on the day of their preparation (0.5% and 0.3%, respectively). The additional advantage of the designed BioTexPants solution is using non-woven fabric as an outer part of the insert, as non-woven fabrics are widely used as PPE in the medical sector. In the medical field, non-woven garments, wipes, and ancillary fabrics are attractive for their sufficient mechanical protection and excellent viral/bacterial barrier properties, while their disposable nature renders it easy to stay sanitary. Non-woven layers are highly absorbent, skin compatible, and air and moisture permeable [42]. Biotextronics is one of the rapidly growing fields, and the biosensor market is forecasted to reach 5 billion per year by 2025 [43]. The use of biotextronics systems is mainly based on monitoring the body's vital signals (e.g., respiratory function, heart rate, body temperature, blood pressure, or neuromuscular response) and their dynamic changes [42; 43]. Biotextronics systems supporting the diagnosis, therapy, and rehabilitation of patients are less often proposed [44]. The designed BioTexPants system is dedicated to patients suffering from recurrent urological infections, which fits into this narrow area of therapeutic biotextronics and supporting therapy. Our system is ultimately an attempt to modulate the microbiota of patients with the reduction or elimination of pathogenic microorganisms. The innovation of the system, apart from its usability, lies in the interaction between the active agent (essential oil) and pathogens of the lower urinary tract in order to achieve a therapeutic effect. To our knowledge, the presented research is one of the few applied to a tetronics system to actively affect microorganisms colonizing the human body. There are solutions limited only to the use of a tetronics sensor for _S. aureus_ detection on human skin [45]. Due to the essential oil action in the gaseous phase, the assessment of the qualitative and quantitative composition of essential oil constituents in the suprasurface phase of the BioTexPants insert is crucial here. According to the literature data, chamomile essential oil has antibacterial activity mainly due to the content of \(\alpha\)-bisabolol and chamazulene; it can be concluded that the best effect in inhibiting bacterial growth should be demonstrated by the inserts on the day of their preparation, and to a lesser extent (due to the lack of chamazulene detection), inserts EO:C 1:2 and 1:3; EO:MC 1:1 and 1:2 after 7 days of storage; EO:C 1:3 and all variants with microcrystalline cellulose after 14 days of storage; and EO:C 1:3 after 28 and 56 days of storage. Since the micro-atmosphere above the surface of the insert immediately after the application of chamomile oil was composed of 14 to 19 oil constituents, regardless of the type of cellulose and the content of the oil, it is advisable to apply the oil to the surface of the insert directly before the use of the biotextronics system.