## 3 Results and Discussions
### Chemical Composition of Whole Fruit and Pulp of S. melongena
#### 3.3.2 Profile in Non-Anthocyanin and Anthocyanin Phenolic Compounds
This sample showed MIC and MFC values of 8.00 and 1.00 mg/mL for _P. fumiculosum_ and _P. ochrochloron_, respectively. Other authors [10; 67] studied the antimicrobial action of _S. melongena_ (fruits and/or leaves) and the results evidenced antibacterial and antifungal potential. Regarding the results obtained for the cytotoxicity and hepatotoxicity assays (Table 8), it was clear that the epicarp of _S. melongena_ fruit was the only hydroethanolic extract that revealed an inhibitory potential (GI\({}_{50}\) < 400 \(\upmu\)g/mL), showing its anti-proliferative capacity in the HeLa, NCI H460, and HepG2 tumor lines. This potential was demonstrated by GI\({}_{50}\) values of 337 \(\pm\) 7 \(\upmu\)g/mL for the HeLa cell line, 338 \(\pm\) 16 \(\upmu\)g/mL for the NCI H460 cell line and, finally, 284 \(\pm\) 10 \(\upmu\)g/mL for the HepG2 cell line. The absence of toxicity of all the studied samples (GI\({}_{50}\) > 400 \(\upmu\)g/mL) was also evident, through the assessment of hepatotoxicityoxicity where a culture of primary non-tumor cells (PLP2) was used. [PERSON] et al. [66] also studied this matrix and evaluated the hepatotoxicityective potential of five varieties of _S. melongena_ obtained in Thailand (SM1: uniform, purple, moderate size; SM2: white and green, moderate size; SM3: elongated and green; SM4: striped, green, moderate size; and SM5: uniform, light green, small size). For this purpose, the HepG2 cell line was used and the results demonstrated a hepatotoxicityective effect in all samples evaluated. \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline  & & & **Antibacterial Activity** & & & \\ \hline
**Extracts** & & _S.a._ & _L.m._ & _E.c._ & _En.cl._ & S.t. \\ \hline Epicarp & MIC & 4.00 & 4.00 & 2.00 & 4.00 & 4.00 \\ MBC & MBC & 8.00 & 8.00 & 4.00 & 8.00 & 8.00 \\ \hline Whole fruit & MIC & 4.00 & 4.00 & 2.00 & 4.00 & 4.00 \\ MBC & MBC & 8.00 & 8.00 & 4.00 & 8.00 & 8.00 \\ \hline Pulp & MIC & 4.00 & 4.00 & 2.00 & 8.00 & 8.00 \\ MBC & MBC & 8.00 & 8.00 & 4.00 & 8.00 & 8.00 \\ \hline Ampicillin & MIC & 0.012 & 0.40 & 0.40 & 0.006 & 0.75 \\ MBC & MBC & 0.025 & 0.50 & 0.50 & 0.012 & 1.20 \\ \hline \hline \multicolumn{7}{c}{**Antifungal Activity**} \\ \hline
**Extracts** & & _A.fum._ & _A.v._ & _A.n._ & _P.f._ & _P.o._ & _T.v._ \\ \hline Epicarp & MIC & >8.00 & >8.00 & >8.00 & >8.00 & >8.00 & >8.00 \\ MBC & MFC & >8.00 & >8.00 & >8.00 & >8.00 & >8.00 \\ \hline Whole fruit & MIC & >8.00 & >8.00 & >8.00 & >8.00 & >8.00 & >8.00 \\ MBC & MFC & >8.00 & >8.00 & >8.00 & 8.00 & 1.00 & >8.00 \\ \hline Pulp & MIC & >8.00 & >8.00 & >8.00 & 8.00 & 1.00 & >8.00 \\ MBC & MFC & >8.00 & >8.00 & >8.00 & 8.00 & 1.00 & >8.00 \\ Ketoconazole & MIC & 0.20 & 0.20 & 0.20 & 0.20 & 0.20 & 0.20 \\ MFC & 0.50 & 0.50 & 0.50 & 0.50 & 0.50 & 0.30 \\ \hline \hline \end{tabular}  
* S.a.: _Staphylococcus aureus_; L.m.: _Listeria monocytogenes_; E.c.: _Escherichia coli_; En.cl.: _Enterobacter cloazer_; St.: _Salmonella Typhimurium; Atun.: _A.Spergillus fumigatus_; Av.: _Asppergillus versicolor_; A.n.: _Asppergillus niger_; P.f.: _Penicillium fumiculosum_; P.o.: _Penicillium ochrochloron_; T.v.: _Trichoderm viride_. MIC: minimum inhibitory concentration, MRC: minimum bactericidal concentration; MFC: minimum fungicidal concentration.  
\end{table} 
Table 7: Antibacterial (MIC and MBC mg/mL) and antifungal (MIC and MFC mg/mL) activity of _S. melongena_ extracts (whole fruit, epicarp, and pulp). Optimization of the Process of Obtaining a Natural Colorant Extract Based on Anthocyanins from the S. melongena Epicarp