## 1 Introduction
The reactive phosphate group reacts with the calcium ions of hydroxyapatite and forms stable calcium phosphate salts [9]. The other reactive site is a photopolymerizable reactive group that reacts with the monomers of resin-based cement or resin-based composite components during the light curing. [PERSON] et al. revealed that the 10-MDP forms a self-assembled monolayer in 4 nm thickness on the surface of synthetic hydroxylapatite. In this layer the phosphate groups directed away from each other, the methacrylate groups directed toward each other [9]. This molecule is developed by Kuraray co. Ltd. The numbers and intensity of the studies are increased further because the patents of the monomer have expired. The most important challenge is the purity of the 10-MDP in its synthesis [10]. The components of a thin bone section are bone replacement material and/or dental implants, bone tissue, and any kind of embedding material. These components should be attached strongly to the microscopic slide and adhesion force should withstand the shear force that acts during the grinding of the section. The appropriate adhesion between the surfaces of the microscope slide and section is a determining factor to achieve an effective microscopic analysis of the histological section. The main chemical composition of a microscope glass slide and dental glass-ceramics is silica. More parallels are found in the composition of adherents (undecalcified thin bone, titanium, or bone replacement material) and glass substratum that led to 10-MDP application in histotechnology. The micromechanical interlocking is commonly used next to the chemical adhesion in the adhesive bonding procedure that is usually achieved by a frosting of the surface. In the case of a well moisturizing bond, the surface irregularities are not going to disturb the microscopy evaluation. [PERSON] et al. made attempts to improve the quality of the final section for light and scanning electron microscopy examinations by hydrofluoric acid treatment of glass slide with silane and photopolymerizable adhesive treatments. The final thickness of the section was 100 \(\upmu\)m \(\pm\) 5 \(\upmu\)m [11]. In the histotechnology of undecalcified thin bone section, the expected, optimal section thickness is one cell thick (under 10 \(\upmu\)m) for high-quality microscopy analysis. The strong mineral acids, like hydrofluoric acid or nitric acid, are used to etch and clean the glass surface. The result of the acid etching is a rough surface glass. The other consequence of acid etching of glass is corrosion, which means alkali ions release from the crystalline structure of the glass. The places of the alkali ions will be replaced by H\({}^{+}\) ions to form Si-OH bonds resulted in increasing of Si-O bond percentage [12]. Nitric acid is a stronger acid than hydrofluoric acid based on the pKa values, therefore, stronger glass protonation can be achieved by nitric acid than hydrofluoric acid. On the other hand, hydrofluoric acid can react with Si-O bonds which leads to the formation of hexafluorosilicic acid that decreases the numbers of Si-O bonds that are needed for protonation. [PERSON] et al. investigated the effects of nitric acid chemical etching on different parameters (like chemical composition, surface roughness, and optical transmittance) of the glass [13], those surface properties are important in adhesive bonding. They found that the optical transmittance of the glass was not affected by chemical etching, the surface roughness increased in the nanometer scale, and acid treatment caused dealkalization on glass similar to hydrofluoric acid. In our research study, the combination of 10-MDP active molecule and nitric acid etched glass surface was used in the adhesive technique of histotechnology to improve bond strengths between the glass slide and the undecalcified bone section and the microscopic evaluation of the section.