## 4 Discussion
As shown in the present study, the essential oil from lettuce leaf basil showed moderate AChE inhibition (IC\({}_{50}\) value from 38.31 \(\pm\) 3.30 to 16.89 \(\pm\) 1.08 \(\mu\)L/mL)-Table 1. These results correspond to the findings reported by [PERSON] et al. [7] and [PERSON], [PERSON], [PERSON], [PERSON], and [PERSON] [8]. It should also be noted that the elicitation with JA in our study significantly improved the ability of lettuce leaf basil EOs to inhibit AChE, and the best result was achieved in the case of the JA2 sample (Table 1). There are no reports on the influence of jasmonic acid elicitation on the ability of plant essential oils to inhibit AChE in the available literature. Other reports on AChE inhibition by some compounds of essential oils indicated that the anticholinesterase inhibition activity of essential oils could especially be associated with monoterpenes, e.g., 1,8-cineole, camphor, \(\alpha\)-pinene, \(\beta\)-pinene, borneol, linalool, menthone, carvone, anethole, anisole, and geraniol [7; 8]. The analysis of the composition of essential oil from basil elicited with jasmonic acid compared to the control basil essential oil presented in our earlier publication emphasizes a partial correlation between the content of essential oil components indicated above (responsible for the inhibition of acetylcholinesterasease) and the ability of the tested essential oils to inhibit this enzyme. It should be noted that the elicitation with jasmonic acid increased the content of 1,8-cineole (JA1 and JA2 sample), \(\alpha\)-pinene (JA1 sample), and \(\beta\)-pinene (JA1 sample). Additionally, geraniol was identified in the essential oil from basil elicited with 1 \(\mu\)m jasmonic acid (JA2 sample), which was not detected in the control sample [22]. However, the correlation analysis revealed no significant correlation between the content of these compounds and anti-acetylcholinesterasesterase activity (data not published). The very high ability of 1,8-cineole to inhibit acetylcholinesteraseinesterase activity was confirmed in a study conducted by [PERSON] et al. [21]. Furthermore, some in vivo studies confirmed the properties of _Ocimmu basilicum_ L. essential oil that may be helpful in the treatment of Alzheimer's disease and other conditions [14]. The results obtained in the current study indicate that the elicitation with jasmonic acid can be an effective method for the enhancement in these properties of basil essential oil (Table 1). The treatment of neurodegenerative diseases that most often affect elderly people is now an enormous challenge for healthcare. These problems are often related to such metabolic disturbances as obesity and type 2 diabetes mellitus, which are part of metabolic syndrome (MS) [31]. MS has become a public health problem, as it may influence the health status both in adults and in children and increase the risk of development of neurodegenerative diseases in the future [32]. There are some enzymes involved in these diseases, and inhibition of their activity could help in the prevention or treatment of metabolic syndrome-related conditions. Some secondary metabolites, e.g., phenolic compounds or compounds of herbal essential oils, were reported to modulate the activity of some digestive enzymes, such as \(\alpha\)-glucosidase, \(\alpha\)-amylase, and lipase [9; 33]. Obesity is associated with an imbalance between the organism's demand for calories and their supply with food. This leads to the disproportional accumulation of adipose tissue, its excessive activity, and disorders in fat metabolism [34]. One of the methods for prevention and treatment of this disease is to reduce lipid hydrolysis in the gastrointestinal tract. To achieve this goal, the activity of pancreatic lipase (triacylglycerol lipase, EC 3.1.1.3) must be inhibited. It is the main enzyme involved in lipid hydrolysis and responsible for the digestion of 50-70% of fats consumed with diet [35].