## Materials and Methods
### Statistical Analysis
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multicolumn{2}{c}{**Genotoxic Treatment**} & \multicolumn{5}{c}{**Ascorbic Acid Antigenotoxic Post-Treatment**} \\ \hline  & \multicolumn{2}{c}{**Migration**} & \multicolumn{2}{c}{**Migration**} \\  & \multicolumn{2}{c}{**Migration**} & \multicolumn{2}{c}{**Group**} & \multicolumn{2}{c}{**Mean Tail**} & \multicolumn{2}{c}{**Number of**} & \multicolumn{2}{c}{**Migration**} \\  & \multicolumn{2}{c}{**Migration**} & \multicolumn{2}{c}{**Containing the Highest Comet**} & \multicolumn{2}{c}{**Length**} & \multicolumn{2}{c}{**Migration**} & \multicolumn{2}{c}{**Containing the Highest Comet**} \\  & \multicolumn{2}{c}{**Cells Number**} & & & & \\ \hline NC & 9.6 & 155 & 6.47 & 9.62 * & 155 & 6.47 \\ \hline EMS 2.5 mM & 14.87 & 108 * & 8.7 * & & & \\ \hline EMS 5 mM & 16.87 & 118 * & 9.96 * & & & \\ \hline EMS 10 mM & 14.12 & 106 & 9.2 * & & & \\ \hline UV-C 1 min & 12.75 & 182 & 7.04 * & & & \\ \hline UV-C 3 min & 14.87 & 130 & 8.19 * & & & \\ \hline UV-C 5 min & 13.87 & 153 & 8.8 * & & & \\ \hline PC (UV-C 5 min) & 13.87 & & 10.87 & 153 & 7.04 \\ \hline UV-C, 5 min + AA 5 mM & & & 9.6 * & 178 * & 7.43 * \\ \hline UV-C, 5 min + AA 10 mM & & & 10.25 & 251 * & 7.33 \\ \hline UV-C, 5 min + AA 15 mM & & & 13.87 & 260 * & 8.28 * \\ \hline \hline \end{tabular}  
* Significant differences (\(p<0.05\)) regarding the negative control.  
\end{table} 
Table 1: Genotoxic and antigenotoxic treatments in _Oreochromis niloticus_ erythrocytes. The genetic damage was observed using the number of MGs, migration groups with the highest comet cell number, and the mean tail length observed in each treatment. 15 mM are significantly different from the negative control (\(p<0.05\)), but a reduction in genetic damage with respect to the PC is observed in the parameter tail length. In the MG (3E), UV-C treatment 5 min + AA 10 mM caused a statistically significant reduction in genetic damage compared to the positive control (\(p<0.05\)), even at the basal level, since no statistical difference was observed with respect to the negative control (\(p<0.05\)). In _Ambystoma mexicanum_, the tail moment and tail length (Figure 2B,D) showed significant differences in the different treatments with respect to the negative control (\(p<0.05\)), but only the post-treatment AA 15 mM reduced the genetic damage compared to the positive control (\(p<0.05\)), although it did not reach the level of basal genetic damage. In the MG, no significant differences were observed between the treatments. Figure 2: Genetic damage observed using the parameters: tail moment, tail length, and MGs in comet cells of _Oreochromis miloticus_ (**A**,**C**,**E**) and Ambystoma mexicanum (**B**,**D**,**F**) exposed post-treatment to UV-C 5 min (PC) and UV-C + AA. * Significant differences (\(p<0.05\)) regarding the negative control. The genetic damage induced by EMS and UV-C and the protective effect of ascorbic acid after exposure of _Ambystoma mexicanum_ erythrocytes to these agents is presented in Table 2. UV-C genotoxic treatment in _Ambystoma mexicanum_ erythrocytes significantly increased (\(p<0.05\)) the average length of the tail, but not the MG with respect to the NC. No significant differences with respect to NC were observed in the MG containing the highest number of comet cells. The EMS showed practically the same behavior, but in the 10 mM EMS, a significant decrease was observed (\(p<0.05\)) with respect to the NC in the number of cells of the migration group that contained the highest number of comets. The antigenotoxic treatment with different concentrations of AA after exposing the cells to 5 min of UV-C showed a significant decrease (\(p<0.05\)) with respect to PC in the number of MGs in all concentrations. The tail length decreased genetic damage compared to PC only for 5 min UV-C + 15 mM AA. The number of MGs containing the highest number of comet cells significantly increased (\(p<0.05\)) compared to PC.