## 4 Conclusions  
This study investigated the physical and chemical properties of WMA mixtures by modifying asphalt binders with the biodegradable Cecabase RT BIO10 additive. The Taguchi method identified the optimal conditions for each test, which were combined into a single overall optimal response, and determined as A3 (0.4%), B4 (140 \({}^{\circ}\)C), C4 (30 min), and D1 (1000 rpm). The achieved 20 \({}^{\circ}\)C reduction in the mixing temperature contributed to sustainable pavement construction and lower GHG emissions. Based on the test results and the analysis conducted, the following conclusions can be drawn. The values at the optimum levels that maximized penetration before and after aging were 75.79 (0.1 mm) and 46.91, representing increases of 12.88% and 20.68%, respectively, compared to virgin bitumen, which indicates better aging resistance and long-term performance. The penetration value before and after aging increased at a 0.4% additive ratio and up to a 2000 rpm mixing speed, while it decreased slightly at a 0.5% additive ratio and higher mixing speeds. Additionally, the penetration value increased with longer mixing times. The values at the optimum levels that minimized the softening point before and after aging were 49.68 \({}^{\circ}\)C and 59.47 \({}^{\circ}\)C, representing decreases of 3.63% and 2.68%, respectively, compared to virgin bitumen, which indicates that the bitumen retains a good balance of  
Figure 13: Estimation results for each type of experiment. flexibility and durability. The mixing temperature, time, and speed influences were lower than the additive ratio. The temperature sensitivity of bitumen decreased with increasing Cecabase RT BIO10 ratios, particularly after aging, resulting in improved resistance of the binders to low-temperature cracking and permanent deformation. The stiffness modulus decreased slightly compared to virgin bitumen at a high service temperature of 40 \({}^{\circ}\)C for all levels of Cecabase RT BIO10. At a low service temperature of \(-\)10 \({}^{\circ}\)C, the stiffness modulus showed a more significant reduction, indicating improved resistance to low-temperature shrinkage. RTFOT mass loss decreased with increasing Cecabase RT BIO10 rates, indicating reduced oxidative aging and hardening of the bitumen. In comparison, mixing temperature, time, and speed had a significantly lower impact than the additive rate. The elastic recovery (maximized optimum) increased with higher additive ratios, temperatures, and mixing times, reaching its maximum at a mixing speed of up to 2000 rpm, resulting in a 30.31% improvement compared to virgin bitumen, which enhanced resistance to permanent deformation at high temperatures and cracking at low temperatures. The Marshall stability value at the optimized levels increased by 31.91% compared to specimens prepared with virgin bitumen. Cecabase RT BIO10 enhanced the seismic modulus of asphalt mixtures, leading to improved performance against fatigue cracking. Both virgin bitumen and bitumen modified with Cecabase RT BIO10 exhibited flash points exceeding the minimum requirement of 230 \({}^{\circ}\)C as specified by ASTM D92. This indicates that modifying bitumen with Cecabase RT BIO10 does not pose any concerns regarding flashpoint, ensuring safety. According to the MRPI results calculated at the selected parameters and levels with 50/70 penetration bitumen used in the study, the optimum levels were found to be 0.4% Cecabase RT BIO10 ratio, 140 \({}^{\circ}\)C mixing temperature, 30 min mixing time, and 1000 RPM mixing speed. The additive offered energy-saving and environmental benefits by lowering the optimum mixing temperature to 140 \({}^{\circ}\)C. Moreover, mixing bitumen at reduced temperatures resulted in less aging and enhanced performance. A radar chart was generated using 1792 estimations with a 95% confidence interval, providing foresight into the expected outcomes at desired parameter levels for each type of experiment, regardless of whether they were included in the experimental plan. Conceptualization, M.C.