# In Vitro Genotoxicity Assessment from the _Glycyrrhiza_ New Variety Extract
## 3 Results
### In Vitro Chromosome Aberration Test  
When WG extract was treated for 6 h with S9 mix, turbidity appeared at the 1400 \(\upmu\)g/mL group. The frequency at which structural aberrations in the negative control group and the WG extract treatment groups appear were 0.33% (negative control group), 0.33% (350 \(\upmu\)g/mL WG extract), 0.00% (700 \(\upmu\)g/mL WG extract), and 0.00% (1400 \(\upmu\)g/mL WG extract). No significant increase in the frequencies of structural aberrations was found in any of the dosed groups. However, the frequency of structural aberrations was significantly higher in the positive control group (17.67%). The frequency of numerical aberrations in the negative control group, the WG extract treatment groups, and the positive control group were 1.00% (negative control group), 0.33% (350 \(\upmu\)g/mL WG extract), 1.00% (700 \(\upmu\)g/mL WG extract), 1.67% (1400 \(\upmu\)g/mL WG extract), and 0.00% (positive control group). We found no significant increase in the frequency of numerical aberrations in any of the dosed groups or in the positive control group compared against the negative control group.  
When the WG extract was treated for 6 h in the absence of S9 mix, precipitation was observed in the 1400 \(\upmu\)g/mL group. Structural aberrations in the negative control group and in the 350, 700, and 1400 \(\upmu\)g/mL WG extract treatment groups were all 0.00%. No significant increase in the frequency of structural aberrations appeared in any of the dosed groups when compared against the negative control group. However, the frequency of structural aberrations was significantly higher in the positive control group (6.33%). Moreover, the frequency of numerical aberrations in the negative control group, the WG extract treatment groups and the positive control group were 1.00% (negativecontrol group), 0.67% (350 \(\upmu\)g/mL WG extract), 0.33% (700 \(\upmu\)g/mL WG extract), 0.33% (1400 \(\upmu\)g/mL WG extract), and 0.33% (positive control group). No significant increase in the frequency of numerical aberrations was observed in any of the dosed groups or the positive control group when compared to the negative control group.  
When the WG extract was treated for 24 h without the metabolic activation, there was no turbidity or precipitation apparent in any of the dosed group. Structural aberrations in the negative control group and the WG extract treatment groups appeared at a rate of 0.00% (negative control group), 0.00% (250 \(\upmu\)g/mL WG extract), 0.00% (500 \(\upmu\)g/mL WG  
\end{table}
Table 1: In vitro Ames test of WG extract.  
extract), 0.00% (1000 \(\upmu\)g/mL WG extract), and 0.33% (1100 \(\upmu\)g/mL WG extract). Structural aberrations did not appear in any of the dosed groups at a statistically significant higher rate than the negative control group. The frequency of structural aberrations was significantly increased in the positive control group (6.33%). The percentage of numerical aberrations in the negative control group, the WG extract treatment groups, and the positive control group was 0.67% (negative control group), 0.00% (250 \(\upmu\)g/mL WG extract), 0.33% (500 \(\upmu\)g/mL WG extract), 0.33% (1000 \(\upmu\)g/mL WG extract), 1.00% (1100 \(\upmu\)g/mL WG extract), and 1.00% (positive control group). In summary, no statistically significant increase in the frequency of numerical aberrations was observed in any of the dosed groups or in the positive control group (Table 2).