# In Vitro Cultures and Volatile Organic Compound Production in _Chiliadenus montanus_ ([PERSON].) [PERSON]
## 3 Results and Discussion
### Volatile Organic Compound Analysis
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & & & & \multicolumn{3}{c}{**Content (\%) 1**} \\ \cline{3-6}
**Compound Name** & **Classification** & **RI- Exp 2** & **RI-Lit 3** & **Wild** & **Microshoot** & **Callus** \\ \hline \(\alpha\)-Pinene & Monoterpene & 946 & 939 & - & - & 13.78 \\ \(\alpha\)-Phellandrene & Monoterpene & 1007 & 1002 & - & - & 3.56 \\ \(\gamma\)-Terpine & Monoterpene & 1011 & 1002 & - & 6.43 & - \\ \(\gamma\)-Terpineol & Monoterpene & 1033 & 1024 & - & 8.25 & 15.13 \\ \(\gamma\)-Terpine & Monoterpene & 1039 & 1037 & - & 1.23 & 22.27 \\ \(\gamma\)-Terpine & Monoterpene & 1054 & 1050 & - & 6.30 & - \\ \(\gamma\)-Terpine & Monoterpene & 1066 & 1059 & - & 0.74 & 1.53 \\ \(\gamma\)-Terpine & Monoterpene & 1079 & 1070 & - & 0.73 & 4.74 \\ Cis-Sabinene hydrate (IPP vs. OH) & Oxygen & 1105 & 1096 & - & 1.00 & - \\ \(6\)-Camphenolle & Monoterpenes & 1109 & 1096 & - & 0.88 & - \\ \(\alpha\)-Terpineol & Oxygen & 1111 & 1102 & - & - & 2.56 \\ Cis-Thujone & Monoterpenes & 1116 & 1114 & - & - & 3.24 \\ Trans-Thujone & Monoterpene & 1130 & 1125 & - & - & 6.45 \\ \(1\)-Undecyne & Monoterpens & 1136 & 1133 & - & 1.50 & - \\ \(\alpha\)-Terpineol & Oxygen & 1159 & 1152 & - & - & 2.10 \\ \(1\),4-Dimethoxybenzene & Non-terpenoid & 1165 & 1165 & - & 6.15 & - \\ Pyrazine, Monoterpene & Monoterpene & 1179 & 1172 & - & 3.5 & - \\ \(2\)-methoxy-3-(1-methylpropyl)- & hydrocarbons & & & & & \\ \(\gamma\)-Terpineol & Oxygen & 1189 & 1189 & - & 1.58 & - \\ \(\alpha\)-Cumenol & Oxygenated & 1203 & 1196 & - & 2.27 & - \\ \(\gamma\)-Terpineol & Oxygenated & 1204 & 1199 & 3.05 & - & 1.51 \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Volatile organic compounds (VOCs) identified in different sample extracts of \(C\). _montanus_ and their content. \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline  & & & & \multicolumn{3}{c}{**Content (\%) 1**} \\ \cline{5-7}
**Compound Name** & **Classification** & **RI- Exp 2** & **RI-Lit 3** & **Wild** & **Microshoot** & **Callus** \\ \hline \(\beta\)-Cyclocitral & Oxygen & 1227 & 1219 & - & 14.3 & - \\ (-) \(-\)Neoisodihydrocarveol & Oxygen & 1234 & 1228 & - & 0.84 & - \\ Thymol, methyl ether & Oxygen & 1237 & 1235 & 9.17 & - & - \\  & monoterepenes & 1257 & 1252 & - & - & 13.25 \\ Thymoquinone & Oxygen & 1258 & 1253 & - & 16.62 & - \\ cis-Myrtanol & Monoterpene & 1274 & 1267 & - & 0.92 & - \\ Geranial & Oxygen & 1282 & 1277 & - & 1.62 & - \\ Isopulegy acetate & Monoterpenes & 1282 & 1277 & - & 1.62 & - \\  & Oxygenated & 1289 & 1283 & - & 2.79 & - \\ neo-is-3-_Thuyl_ acetate & Monoterpene & 1308 & 1298 & 1.68 & - & - \\ Azulene & Oxygenated & 1365 & 1359 & - & - & 3.81 \\ neo-dihydro eugenol & Oxygenated & 1375 & 1369 & - & 6.51 & - \\  & monoterepenes & 1381 & 1376 & 4.25 & - & - \\  & Oxygenated & 1383 & 1381 & - & 3.76 & - \\  & Oxygenated & 1384 & 1384 & - & - & 4.43 \\ Dihydro eugenol & Sesquiterpene & 1425 & 1419 & 5.15 & - & - \\ \(\alpha\)-Copaene & Sesquiterpene & 1436 & 1432 & 0.56 & - & - \\ Geranyl acetate & Sesquiterpene & 1462 & 1454 & 6.77 & 4.53 & - \\  & hydrocarbons & 1467 & 1460 & 11.92 & - & - \\ Isobornyl propionate & Sesquiterpene & 1475 & 1470 & 1.59 & 1.95 & - \\ \(\beta\)-Caroadiene & Sesquiterpene & 1480 & 1476 & 7.54 & - & - \\ trans-Cadina-1(6),4-diene & Sesquiterpene & 1493 & 1493 & 1.01 & - & - \\ \(\alpha\)-Zingiberen & Oxygenated & 1503 & 1495 & 4.35 & - & - \\  & sesquiterpene & 1512 & 1506 & 0.61 & - & - \\ Premnaspirodiene & Sesquiterpene & 1519 & 1512 & 9.32 & 2.35 & - \\ \(\delta\)-_amorphene_ & Sesquiterpene & 1521 & 1513 & - & 1.33 & - \\  & hydrocarbons & 1523 & 1522 & 1.70 & - & - \\ trans-Calamenene & Sesquiterpene & 1528 & 1522 & 2.52 & - & - \\ Spathulenol & Oxygenated & 1578 & 1578 & 2.22 & - & - \\  & sesquiterpenenes & 1528 & 1522 & 1.70 & - & - \\  & & & & & & \\ \end{tabular}
\end{table} 
Table 4: _Cont._The composition of the microshoot, wild, and callus VOCs showed qualitative and quantitative differences.