## 2 Results and Discussion
### MCF7 Human Breast Cancer Cell Line  
Analyzing the effect of different extracts from cookies with the addition of selected wild grown fruits on their antitumor capability against the human breast MCF-7 cancer line, no effect of the tested extracts was observed (\(p>0.05\)). In most cases, null values were obtained (Table 3). As in the previous cancer cell line, sea buckthorn extracts were an exception again. Although relatively low, the cytotoxic effect of it was variable within the concentrations used (Table 3). The highest cytotoxic effect was shown by its extracts at concentrations of 0.5 mg/mL--3.87% and 1.5 mg/mL--5.77% (\(p<0.05\)). Interestingly, when assessing the viability of MCF7 cancer cells, a differentiated effect of individual extracts was documented (Table 3, Figure 2). Samples containing chokeberries and elderberries had the greatest effect on cancer cell viability, regardless of the dose used, resulting in the lowest percentage of cell vitality, average 38% and 39%, respectively (Table 3).  
\begin{table}
\begin{tabular}{c c c c} \hline  & & **Cytotoxicity 1** & **Viability 1** \\ \hline
**Extract Type** & **Extract Concentration** & **Mean Value [\%] \(\pm\) Standard Deviation** \\  & **[mg/mL]** & **Mean Value [\%] \(\pm\) Standard Deviation** \\ \hline Chokeberry & 0.5 & 4.51 \({}^{\text{a}}\)\(\pm\) 0.06 & 96.78 \({}^{\text{a}}\)\(\pm\) 0.05 \\ Chokeberry & 1.0 & 3.73 \({}^{\text{a}}\)\(\pm\) 0.02 & 85.54 \({}^{\text{b}}\)\(\pm\) 0.00 \\ (_Aronia melanocarppa_) & 1.5 & 19.38 \({}^{\text{c}}\)\(\pm\) 0.02 & 93.17 \({}^{\text{b}}\)\(\pm\) 0.05 \\  & 2.5 & 4.70 \({}^{\text{f}}\)\(\pm\) 0.01 & 89.37 \({}^{\text{b}}\)\(\pm\) 0.02 \\ \hline  & \(x\) & 8.08 & 91.35 \\ \hline Control & 0.5 & 2.85 \({}^{\text{a}}\)\(\pm\) 0.06 & 97.22 \({}^{\text{a}}\)\(\pm\) 0.00 \\  & 1.0 & 3.07 \({}^{\text{a}}\)\(\pm\) 0.01 & 91.50 \({}^{\text{a}}\)\(\pm\) 0.10 \\  & 1.5 & 11.22 \({}^{\text{c}}\)\(\pm\) 0.01 & 82.50 \({}^{\text{a}}\)\(\pm\) 0.06 \\  & 2.5 & 7.33 \({}^{\text{a}}\)\(\pm\) 0.01 & 94.17 \({}^{\text{a}}\)\(\pm\) 0.02 \\ \hline  & \(x\) & 8.99 & 91.21 \\ \hline \end{tabular} \({}^{1}\) The values of the superscript with different letters in columns are significantly different at \(p<0.05\).  
\end{table}
Table 2: _Cont._  
Figure 1: Cytotoxicity of different plant extracts on WM793 cell line depending on the kind of wild grown-fruits enriched cookies.  
\begin{table}
\begin{tabular}{c c c c} \hline \hline  & \multicolumn{2}{c}{**Cytotoxicity 1**} & \multicolumn{2}{c}{**Viability 1**} \\ \hline
**Extract Type** & \multicolumn{2}{c}{**Extract Concentration**} & \multicolumn{2}{c}{**Mean Value [\%] \(\pm\) Standard Deviation**} \\ \hline \multirow{3}{*}{\begin{tabular}{c} Sea-buckthorn \\ (_Hippphae rhamnoides_ L.) \\ \end{tabular} } & 0.5 & 3.87 \({}^{\text{b}}\)\(\pm\) 0.08 & 89.19 \({}^{\text{b}}\)\(\pm\) 0.06 \\  & 1.0 & 0.94 \({}^{\text{a}}\)\(\pm\) 0.03 & 84.79 \({}^{\text{b}}\)\(\pm\) 0.08 \\  & 1.5 & 5.77 \({}^{\text{b}}\)\(\pm\) 0.08 & 81.58 \({}^{\text{b}}\)\(\pm\) 0.08 \\  & 2.5 & 1.36 \({}^{\text{a}}\)\(\pm\) 0.02 & 85.78 \({}^{\text{b}}\)\(\pm\) 0.04 \\ \hline \multirow{3}{*}{\begin{tabular}{c} Hawthorn \\ (_Centaagus L.) \\ \end{tabular} } & 0.5 & 0.0 & 66.59 \({}^{\text{b}}\)\(\pm\) 0.09 \\  & 1.0 & 0.0 & 64.78 \({}^{\text{b}}\)\(\pm\) 0.01 \\  & 1.5 & 0.0 & 57.02 \({}^{\text{b}}\)\(\pm\) 0.06 \\  & 2.5 & 0.0 & 58.55 \({}^{\text{b}}\)\(\pm\) 0.09 \\ \hline \multirow{3}{*}{\
\begin{tabular}{c} Elderberry \\ (_Sambucus nigra_ L.) \\ \end{tabular} } & 0.5 & 0.0 & 41.55 \({}^{\text{a}}\)\(\pm\) 0.06 \\  & 1.0 & 0.0 & 38.04 \({}^{\text{a}}\)\(\pm\) 0.03 \\  & 1.5 & 0.0 & 40.02 \({}^{\text{a}}\)\(\pm\) 0.04 \\  & 2.5 & 0.0 & 38.13 \({}^{\text{a}}\)\(\pm\) 0.03 \\ \hline \multirow{3}{*}{
\begin{tabular}{c} Elderberry \\ (_Sambucus nigra_ L.) \\ \end{tabular} } & \multicolumn{2}{c}{-} & 39.43 \\ \cline{2-2}