The classical transgenesis technique involves the introduction of additional copies of the key enzyme-encoding genes into the plant genome, either from the same plant's own genome or from other species. The method of classical transgenesis is widely used to create overexpressing lines, which are plants with significantly higher-than-normal expression of a specific gene. For this, additional copies of the gene are added, controlledby constitutive promoters which are always active, such as the cauliflower mosaic virus (CaMV) 35S promoter. Classical transgenesis is also commonly used to create plants, notably _Arabidopsis thaliana_ transgenic lines with knocked out genes, where T-DNA insertions, falling randomly into the plant's own gene regions, disrupted their functions. Since insertion in these plants acts as a marker that allows cloning of DNA sections adjacent to the insertion, such plants are of interest for identifying genes, the function of which has been disrupted by the insertion. However, large-scale analyses of the composition of nucleotide sequences of extended DNA sections adjacent to the insertions revealed genetic disorders, such as translocations, inversions, and deletions, in some cases. These disorders can also act as sources of various phenotypic disorders and mask the phenotypic manifestations of mutations directly caused by T-DNA insertions. In addition, based on the results of a detailed study, in some cases, various disorders were detected away from the integration site of the foreign gene in such mutant plants. Additionally, this approach proved challenging in plant species lacking a wide range of knockout mutants, unlike Arabidopsis. Another approach to regulate gene expression is RNA interference (RNAi). This method is based on the creation of genetic constructs with fragments of the coding region of the target gene linked to an antisense sequence. This promotes hairpin RNA formation after transcription, leading to the production of interfering RNAs that suppress gene expression at the post-transcriptional level. This is the so-called \"gene knockdown\" technique, which most often does not lead to complete suppression of gene expression. It is a convenient tool in cases where a partial reduction in gene expression is preferable, e.g., when a complete gene knockout could cause serious metabolic disorders. The genome editing strategies, i.e., site-directed mutagenesis techniques, incorporate the use of zinc finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN), and clustered regularly interspaced short palindromic repeats with CRISPR-associated protein (CRISPRR/Cas) systems. CRISPR/Cas9 has become the most popular system due to its simplicity, accuracy, and versatility. This method uses ribonucleoprotein complexes that recognize targeted sequences in the genome. These complexes use synthetic guide RNA to direct a double-stranded DNA break. This break is then repaired either through homology-directed repair (HDR) using a donor template or through non-homologous end joining (NHEJ). The most common results of NHEJ are insertions and deletions (indels) in target sites, often leading to frame shift mutations in the coding sequence of the gene. This can result in the introduction of a stop codon, potentially leading to loss of gene function or, in some rare cases, to enhanced gene expression, for example, through a nonsense-mediated mRNA decay (NMD), by affecting alternative splicing (for review, see [12]). Thus, genome edited plants are increasingly displacing mutants with T-DNA insertions, and the CRISPR/Cas9 genome editing system has become widely used in applied research to create crops with valuable agronomic traits without introducing exogenous sequences and with minimal off-target changes in the genome [13; 14]. Modern actively developing approaches designed on CRISPR/Cas9 provide the delivery of effector molecules or markers to certain DNA regions to change the transcriptional level of gene expression towards activation (CRISPRa) or suppression of gene activity (CRISPRR).