# Utilization of Wool Integral Lipids to Determine Milk Fat Content in Suffolk Down Ewes
## 3 Results
### Wool Fat and Fatty Acid Composition
To determine the relationship between wool FAs and milk fat content, the correlations between each classification period, according to the milk fat variable, were calculated  
\begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline \multirow{2}{*}{**Fatty Acids**} & \multicolumn{4}{c}{**30 Days in Milk**} & \multicolumn{4}{c}{**60 Days in Milk**} \\ \cline{2-7}  & \multicolumn{2}{c}{**Low 1**} & \multicolumn{2}{c}{**High 2**} & \multirow{2}{*}{\(p\)**-Value**} & \multicolumn{2}{c}{**Low 1**} & \multicolumn{2}{c}{**High 2**} & \multirow{2}{*}{\(p\)**-Value**} \\  & & **(_**_**_**_**_**_**_**_**_**_**_**_**_**_**_**_**_**_**_**_**_**_(_**_  
(Table 3). For each classification, C18:1n9c, C18:2n6c, and C22:2 were the only FAs that demonstrated a positive correlation between each classification period (30 and 60 days) and milk fat content. Furthermore, in the first period of 30 days in milk, positive correlations were determined in SFAs (C14:0 and C24:0) and PUFA (C20:3n6). For the FA proportions and DI, only the PUFA content and DI C18 exhibited a positive correlation with milk fat content. Moreover, negative correlations were determined for SFA (C15:0 and C18:0), MUFA (C14:1), and DI C14 with milk fat content. In the second period of 60 days, the SFAs (C10:0, C12:0, C13:0, C15:0, and C16:0), MUFAs (C14:1, C16:1, and C24:1n9), and PUFA (C18:3n3 and C22:6n3) were positively correlated with milk fat content. Moreover, the proportions of all FAs, i.e., SFA, MUFA, and PUFA, and DI C18 were shown to have a positive correlation with milk fat content in this period, with the exception of the FA C20:3n6, which exhibited a negative correlation. All FAs were evaluated to perform a discriminant analysis. However, the wool FAs C18:1n9c, C18:2n6c, and C22:2 were the most discriminating variables for the classification of high milk fat content (Table 4). In the validation, the FA C18:1n9c demonstrated an accuracy of 79.17% as a single factor, which was indicated by the fact that, in the evaluation of 24 ewes, nine ewes were correctly classified as having a high milk fat content, three as false positives, two as false negatives, and 10 as true negatives. The predictions based on the combination of this FA with either C18:2n6c or C22:2 revealed accuracies of 87.50% and 70.83%, respectively. However, the combination of all FAs gave an accuracy of 87.50%. Moreover, the MCC exhibited high scores for the combination of C18:1n9c and C18:2n6c (MCC = 0.75), followed by C18:1n9c as a single factor with an MCC value of 0.58. \begin{table}
\begin{tabular}{c c c} \hline \hline \multirow{2}{*}{**Fatty Acids**} & \multicolumn{3}{c}{**Milk Fat Content**} \\ \cline{2-3}  & **30 Days in Milk** & **60 Days in Milk** \\ \hline C10:0 & 0.005 & 0.528 ** \\ C12:0 & \(-\)0.177 & 0.263 * \\ C13:0 & 0.035 & 0.547 ** \\ C14:0 & 0.570 ** & 0.012 \\ C14:1 & \(-\)0.527 ** & 0.374 ** \\ C15:0 & \(-\)0.356 ** & 0.264 * \\ C16:0 & \(-\)0.144 & 0.599 ** \\ C16:1 & \(-\)0.194 & 0.535 ** \\ C17:0 & \(-\)0.157 & \(-\)0.027 \\ C18:0 & \(-\)0.246 * & 0.646 ** \\ C18:1n9c & 0.699 ** & 0.640 ** \\ C18:2n6c & 0.552 ** & 0.656 ** \\ C20:0 & 0.209 & 0.204 \\ C18:3n3 & \(-\)0.142 & 0.335 ** \\ C22:0 & 0.011 & \(-\)0.006 \\ C20:3n6 & 0.463 ** & \(-\)0.258 * \\ C22:2 & 0.438 ** & 0.395 ** \\ C24:0 & 0.251 * & 0.137 \\ C24:1n9 & \(-\)0.070 & 0.299 ** \\ C22:6n3 & 0.196 & 0.229 ** \\ SFA & \(-\)0.120 & 0.628 ** \\ MUFA & 0.189 & 0.566 ** \\ PUFA & 0.533 ** & 0.497 ** \\ DI C14 & \(-\)0.633 ** & 0.166 \\ DI C16 & \(-\)0.050 & 0.191 \\ DI C18 & 0.698 ** & 0.271 * \\ Wool fat & \(-\)0.150 & 0.149 \\ \hline \hline \end{tabular}
\end{table} 
Table 3: [PERSON]’s correlation coefficients among wool fatty acid content and milk fat content.