## 3 Results
### Exoproteomes Analysis During Semi-Solid Cultivation on Media Containing Various Types of Sawdust
Lactic acid was also detected on all the media and glycolic (2-hydroxy acetic) acid was identified on the media containing alder and pine sawdust. Although no amino acids were found among the metabolites, hydroxy fatty acids, i.e., intermediates in the metabolism of branched-chain amino acids--alpha-hydroxysiovaleric acid on the GP medium and 3-hydroxysiobutyric acid or 3-hydroxy-2-methylpropanoic acid--were detected on all the sawdust-containing media. Among the phenolic compounds, benzeneacetaldehyde was found on the GP medium only, while at the end of the fungal cultivation, the total phenol content on all the media was significantly lower than that on the initial ones (Table 2). Wood Degradation Analysis During WRF Crucibulum laee LE-BIN 1700 and Trametes Hirsuta LE-BIN 072 Solid-State Cultivation on Various Types of Sawdust  
Among WRF wood-destroying Basidiomycetes, _Trametes_ spp. (_Polvoraceae_ family) are reference organisms able to efficiently biodegrade all wood components ( lignin, cellulose and hemicelluloses) [40]. On the contrary, the ability of white rot fungi (_Nidurulaceae_ family) to destroy the complex structure of plant biopolymers is still under investigation. In order to investigate the saprotrophic wood decomposition ability of C. _leave_ (_Nidurulaceae_ family) and to compare it with that of the primary wood xylotroph _T. hirsuta_, a potent lignocellulosic destructor [41], solid-phase cultivation of both fungal cultures was carried out on different wood sawdust (birch, alder and pine). The cultivation time of _T. hirsuta_ on all the types of sawdust was 60 days; as for _C. laeee_, the cultivation time was extended to 120 days in proportion to a growth rate difference between these two fungi, since we had previously shown that the growth rate of _T. hirsuta_ mycelium on agar media containing birch, alder  
Figure 6: Total pool of identified metabolites in the GP (glucose–peptone medium), GP-B (glucose–peptone with birch sawdust), GP-A (glucose–peptone with alder sawdust) and GP-P (glucose–peptone with pine sawdust) of the _Crucibulum laee_ LE-BIN 1700.  
and pine sawdust is about 16-18 mm/day [30], whereas that of _C. laeev_ is about two times lower (6-9 mm/day) under the same experimental conditions (Figure 1A). Analysis of the mass loss (Figure 7A) and specific gravity variation (Figure 7B) in sawdust after cultivation of the WRF _T. hirsuta_ and _C. laeev_ (Figure 7 top and bottom, respectively) showed that the primary wood xylotroph (_T. hirsuta_) degrades hardwood sawdust more strongly than coniferous sawdust. For example, mass loss of birch and alder sawdust was approximately 25%, and their specific gravity decreased by 32 and 43%, respectively (Figure 7 top). At the same time, the loss of mass and specific gravity of pine sawdust was 10-13%. As for the _C. laeev_ saprotroph, the highest mass loss and specific gravity loss (about 23%) were detected in birch sawdust. The fungus decomposed alder and pine sawdust at the same rate (the loss of mass and specific gravity of alder and pine sawdust were 10-15%), but more slowly than birch sawdust (Figure 7 bottom).