## 3 Results
### Bioassays against Different Bacterial Inoculum Concentrations  
Correlating to the lethal doses found, a dose-dependent effect was observed between _Aedes_ larvae mortality and bacterial inoculum dose. Results found fall in line with previous investigations, as _Ae. aegypti_ was able to tolerate inoculums as high as \(10^{7}\) CFU/mL [51]. A dose-dependent effect was evident among all nursery types (Figure 2), with _Ae. albopictus_ showing greater susceptibility to the bacterial treatment, particularly in the \(10^{5}\)-\(10^{6}\) CFU/mL range. Significant differences were found among the inoculated treatments and the controls for all nursery types except \(10^{4}\) CFU/mL at both 24 h (_Ae. albopictus_: ANOVA: F(4\({}_{10}\)) = 548, \(p<0.0001\). Average larval mortality per inoculum dose: \(10^{4}\): \(0\%\), \(10^{5}\): 55\(\%\), \(10^{6}\): \(96\%\), \(10^{7}\): \(100\%\)_; _Ae. aegypti_: ANOVA: F(4\({}_{10}\)) = 23.54, \(p<0.0001\). Average larval mortality per inoculum dose: \(10^{4}\): \(0\%\), \(10^{5}\): \(15\%\), \(10^{6}\): \(30\%\), \(10^{7}\): \(95\%\); mixed nurseries: ANOVA: F(4\({}_{10}\)) = 104, \(p<0.0001\). Average larval mortality per inoculum dose:  
\begin{table}
\begin{tabular}{c c c} \hline
**Nursery Type** & **LD50 (CFU/mL)** & **LD95 (CFU/mL)** \\ \hline _Ae. aegypti_ & \(1.8\times 10^{7}\) & \(6.3\times 10^{7}\) \\ \hline _Ae. albopictus_ & \(5.9\times 10^{5}\) & \(1.23\times 10^{6}\) \\ \hline Mixed (both species) & \(1.45\times 10^{7}\) & \(3.04\times 10^{7}\) \\ \hline Mixed (_Ae. aegypti_) & \(7.23\times 10^{6}\) & \(3.25\times 10^{7}\) \\ \hline Mixed (_Ae. albopictus_) & \(1.88\times 10^{5}\) & \(9.74\times 10^{5}\) \\ \hline \end{tabular}
\end{table}
Table 2: Lethal _Lysinibacillus sphareicus_ bacterial mixture (III(3)7 + 2362) doses for each of the treatment nurseries.  
Figure 1: (**A**) Modified _E. coli_ expressing the red fluorescent protein (mRFP) after being exposed to antibiotic. (**B**) Colouration observed within the larvae’s digestive tract after the ingestion of modified _E. coli_ expressing mRFP.  
\(10^{4}\): 0%, \(10^{5}\): 35%, \(10^{6}\): 55%, \(10^{7}\): 100%) and 48 h (_Ae. albopictus_: ANOVA: F(4,10) = 337.9, \(p<0.0001\). Average larval mortality per inoculum dose: \(10^{4}\): 10%, \(10^{5}\): 82%, \(10^{6}\): 100%, \(10^{7}\): 100%; _Ae. aegypti_: ANOVA: F(4,10) = 87.6, \(p<0.0001\). Average larval mortality per inoculum dose: \(10^{4}\): 7%, \(10^{5}\): 18%, \(10^{6}\): 45%, \(10^{7}\): 100%; Mixed nurseries: ANOVA: F(4,10) = 257.9, \(p<0.0001\). Average larval mortality per inoculum dose: \(10^{4}\): 8%, \(10^{5}\): 50%, \(10^{6}\): 70%, \(10^{7}\): 100%) past inoculation. Results indicate the presence of a clear dose-dependent relationship. This relationship is further solidified by the results from the overall mortality across all inoculum concentrations (Figure 3), which again showed statistical significance when compared to the control treatments at both 24 h (ANOVA: F(4,40) = 45.54, \(p<0.0001\). Average larval mortality per inoculum dose: \(10^{4}\): 0%, \(10^{5}\): 35%, \(10^{6}\): 55%, \(10^{7}\): 100%) and 48 h (ANOVA: F(4,40) = 50.07, \(p<0.0001\). Average larval mortality per inoculum dose: \(10^{4}\): 8%, \(10^{5}\): 50%, \(10^{6}\): 70%, \(10^{7}\): 100%).  
Figure 2: Dose-dependent responses of the different _Aedes_ nurseries when exposed to the different inoculum concentrations used at the set time intervals. (**A**) _Aedes albopictus_ nurseries (24 h post inoculation). (**B**) _Aedes albopictus_ nurseries (48 h post inoculation). (**C**) _Aedes aegypti_ nurseries (24 h post inoculation). (**D**) _Aedes aegypti_ nurseries (48 h post inoculation). (**E**) Mixed nurseries (24 h post inoculation). (**F**) Mixed nurseries (48 h post inoculation).