# Chromosome Number and Genome Size Evolution in _Brasolia_ and _Sobralia_ (Sobralieaee, Orchidaceae)
## 2 Results
### Chromosome Number and Genome Size Varies in Sobralieae
\begin{table}
\begin{tabular}{c c c c c c c c} \hline \multirow{2}{*}{**No.**} & \multicolumn{2}{c}{**Species [n. FCM**} & \multirow{2}{*}{\begin{tabular}{c} **Chromosome** \\ **Analyzed** \\ **Specimens** \\ \end{tabular} } & \multirow{2}{*}{\begin{tabular}{c} **Figure/** \\ **Reference** \\ \end{tabular} } & \multicolumn{2}{c}{**1C DNA Content**} & \multirow{2}{*}{\
\begin{tabular}{c} **CV Sample** \\ **\%** \\ \end{tabular} } & \multirow{2}{*}{
\begin{tabular}{c} **Genome Size** \\ **Category** \\ \end{tabular} } \\  & & & & & & \\ \hline \multicolumn{8}{c}{_Brasolia_} \\ \hline
01 & _B. altissima_[2] & 46 & Figure 3a &
\begin{tabular}{c} 6.37 \(\pm\) \\ 0.075 \\ \end{tabular} & 6157 & 3.65 & intermediate \\ \hline
02 & _B. cattleva_[1] & 42 & Figure 3b &
\begin{tabular}{c} 4.98 \(\pm\) \\ 0.014 \\ \end{tabular} & 4876 & 3.95 & intermediate \\ \hline
03 & _B. dichotoma_[1] & 46 & Figure 3c &
\begin{tabular}{c} 4.88 \(\pm\) \\ 0.005 \\ \end{tabular} & 4773 & 3.81 & intermediate \\ \hline
04 & _B. flava_[2] & 46 & Figure 3d &
\begin{tabular}{c} 4.97 \(\pm\) \\ 0.028 \\ \end{tabular} & 4854 & 4.14 & intermediate \\ \hline
05 & _B. hirtzii_[2] & 44 & Figure 3e &
\begin{tabular}{c} 4.69 \(\pm\) \\ 0.011 \\ \end{tabular} & 4582 & 3.76 & intermediate \\ \hline
06 & _B. portillae_[3] & 48 & Figure 3f &
\begin{tabular}{c} 3.30 \(\pm\) \\ 0.016 \\ \end{tabular} & 3228 & 4.75 & small \\ \hline \multicolumn{8}{c}{_Ellenanthus_} \\ \hline
07 & _E. brasiliensisis_[3] & 50 &
\begin{tabular}{c} Figure 3g/FG10 \\ [8] \\ \end{tabular} & 4.79 \(\pm\) \\
08 & _E. crinipes_[3] & 50 & Figure 3h &
\begin{tabular}{c} 2.04 \(\pm\) \\ 0.078 \\ \end{tabular} & 1995 & 3.11 & small \\ \hline \multicolumn{8}{c}{_Sobralia_} \\ \hline \multicolumn{8}{c}{**Sect. _Sobralia_**} \\ \hline
09 & _S. atropubescens_[1] & - & - &
\begin{tabular}{c} 4.19 \(\pm\) \\ 0.010 \\ \end{tabular} & 4098 & 5.08 & intermediate \\
10 & _S. bimaculata_ & 44 & Figure 3a & - & - & - & - \\
11 & _[PERSON]. [PERSON]_ & 42, 44 & Figure 3b,c & - & - & - & - \\
12 & _[PERSON]. fimbriata_[1] & 46 & Figure 3d &
\begin{tabular}{c} 4.50 \(\pm\) \\ 0.019 \\ \end{tabular} & 4401 & 4.37 & intermediate \\
13 & _[PERSON]. [PERSON]_ & 48 & FG10 [8] & - & - & - & - \\
14 & _[PERSON]. macantha_[2] & 48 & Figure 3e &
\begin{tabular}{c} 6.24 \(\pm\) \\ 0.121 \\ \end{tabular} & 6108 & 4.15 & intermediate \\
15 & _[PERSON]. powelli_[2] & 44 & Figure 3f &
\begin{tabular}{c} 4.34 \(\pm\) \\ 0.085 \\ \end{tabular} & 4248 & 4.45 & intermediate \\
16 & _S. sessilis_[1] & 48 & FG10 [8] &
\begin{tabular}{c} 4.45 \(\pm\) \\ 0.095 \\ \end{tabular} & 4347 & 4.47 & intermediate \\
17 & _S. wallecuancana_ & 48 & Figure 3g & - & - & - & - \\
18 & _S. wilsoniana_[2] & 48 & Figure 3h &
\begin{tabular}{c} 4.25 \(\pm\) \\ 0.025 \\ \end{tabular} & 4163 & 4.95 & intermediate \\
19 & _S. yuanperyensis_[1] & 46 & Figure 4i &
\begin{tabular}{c} 3.97 \(\pm\) \\ 0.012 \\ \end{tabular} & 3878 & 4.52 & intermediate \\ \hline \end{tabular}
\end{table} 
Table 1: Chromosome number and genome size in Sobralieae. The list of _Brasolia_, _Ellenthus_, and _Sobralia_ species analyzed here and published on the literature is presented. For each species, the determined chromosome number is presented followed by its figure in text or reference on the literature. For each species analyzed here, the number of analyzed specimens in FCM is indicated in brackets. The genome size is presented in pg, followed by its standard deviation (SD), and Mega base-pairs (Mbp). The genome size (the 1C-value) is given in pg \(\pm\) SD (Standard Deviation) and Mbp (Mega base-pairs). For each 1C-value, the sample Coefficient of Variation (CV) is presented and the genome size is classified according [PERSON] et al. [16]. The genome size among _Brasolia_ species ranged from 1C = 6.37 pg in _B. altissima_ to 1C = 3.30 pg in _B. portillae_, representing a two-fold variation. According to 1C-value, _Brasolia_ species could be organized into four groups (F = 3.719, \(p\) < 2 \(\times\) 10\({}^{-16}\); Figure 5a): the first represented by _B.