## 5 Conclusions  
In conclusion, the Luminex xTAG\({}^{\circledgtrgtr}\) GPP and our DFS in-house RT-PCR had high agreements for the detection of _C. difficile_ toxin gene, _tcdB_ with a PPA of 97% and a NPA of 99%. Multianalyte panels such as the Luminex xTAG\({}^{\circledgtrgtr}\) GPP are high volume testing platforms with rapid turnaround times, no special setup, and are less labour intensive while providing identification of multiple different enteric pathogens [7, 8]. This assay can easily be implemented in frontline microbiology laboratories and provides a rapid tool in making a diagnosis for enteric pathogens including _C. difficile_. However, our in-house DFS RT-PCR assay can also be used to detect toxigenic _C. difficile_, which is especially useful in a research setting as it is both sensitive and economical compared to the multianalyte panels or commercial assays available.  
**Supplementary Materials:** The following supporting information can be downloaded at: [[https://www.mdpi.com/article/10.3390/microorganisms10112214/s1](https://www.mdpi.com/article/10.3390/microorganisms10112214/s1)]([https://www.mdpi.com/article/10.3390/microorganisms10112214/s1](https://www.mdpi.com/article/10.3390/microorganisms10112214/s1)), Table S1: Specificity panel for real-time PCR; Table S2: Sensitivity panel for determining the limit of detection of _tcd_ BT-PCR; Table S3: Ct values for _tcd_B from in-house RT-PCR for GPP/ DFS RT-PCR discordant samples.  
**Author Contributions:** Conceptualization, L.C.; methodology, H.T., C.D.L., S.B.N.M. and B.P.; formal analysis, H.T., L.C., J.X., C.D.L., R.Z. and S.B.N.M.; data curation, J.X., R.Z., H.T., S.B.N.M., C.D.L. and B.P.; writing--original draft preparation, H.T. and L.C.; writing--review and editing, S.B.N.M., C.D.L., B.P., T.S., S.B.F., B.E.L., R.Z. and X.-L.P.; supervision, L.C.; project administration, L.C.; funding acquisition, S.B.F., B.E.L., X.-L.P. and L.C. All authors have read and agreed to the published version of the manuscript.  
**Funding:** This work was supported by the Alberta Provincial Pediatric EnTeric Infection TEam (APPETITE), which was funded by a grant from the Alberta Innovates-Health Solutions Team Collaborative Research Innovation Opportunity. APPETITE is also supported through a partnership awards by the Alberta Children's Hospital Research Institute (Calgary, AB, Canada) and the Women and Children's Hospital Research Institute (Edmonton, AB, Canada). SBF is supported by the Alberta Children's Hospital Foundation Professorship in Child Health and Wellness. The Pediatric Emergency Medicine Research Associate Program (PEMRAP) is supported by a grant from the Alberta Children's Hospital Foundation. This study was also supported by Alberta Health Services Residual Funds. HT was supported by Alberta Innovates Summer Research Studentship; CL was the recipient of the Canada Graduate Scholarship, Walter H. Johns Graduate Fellowship and Women and Children's Hospital Research Institute Graduate Studentship.  
**Institutional Review Board Statement:** This study was approved by the ethics boards of the University of Alberta (Approval code: Pro00050790, Approval Date: 2014) and the University of Calgary (Approval code: REB14-1112, Approval Date: 2014).  
**Informed Consent Statement:** Informed consent was obtained from all subjects involved in the study.  
**Data Availability Statement:** Not applicable.  
**Conflicts of Interest:** The authors declare no conflict of interest.