## 3 Results
### Inhibition of Adhesion  
Three-factor ANOVA indicated a significant interaction of the alloy, bacteria, and agent on adhesion reduction with a smaller effect size (\(p=0.001\); \(\eta^{2}=0.084\)). Two-factor ANOVA showed that the interaction of the bacteria and alloy was significant and with a greater effect with Perio Plus (\(p<0.001\); \(\eta^{2}=0.452\)) as well as with pure CHX DG solution (\(p<0.001\); \(\eta^{2}=0.194\)). Curasept showed no significant interactions. Compared to other bacteria, _A. actinomycetemcomitans_ showed the greatest inhibition of adhesion to CHX DG solutions (Figure 3). The inhibition of adhesion was lowest on _S. mutans_ and highest on _A. actinomycetemcomitans_ on both alloys. The effect of CHX DG solution on the inhibition of adhesion was greater with NiTi than with SS (\(\eta^{2}=0.829\) and 0.626) and was significantly different for bacteria. On SS, there was less inhibition of adhesion for _V. parvula_ than for _S. oralis_, and after the application of Curasept, there was less inhibition of adhesion of _V. parvula_ on NiTi (84% vs. 99-100%) (\(p<0.001\); \(\eta^{2}=0.757\)) and SS (78 vs. 98-100%; \(p<0.001\); \(\eta^{2}=0.713\)) than other bacteria. Perio Plus had a weaker effect on the inhibition of _V. parvula_ on NiTi (99.8% 100%) (\(p<0.001\); \(\eta^{2}=0.654\)) with a greater effect size on SS (99.3% vs. 100%; \(p<0.001\); \(\eta^{2}=0.779\)) than it acted on other bacteria with both alloys.  
Viability depended on the type of bacteria, exposure, and alloy combination (\(p=0.047\); \(\eta^{2}=0.217\); Figures 4 and 5). It was smaller with SS wires than with NiTi wires and after CHX DG therapy. Viability was highest in _V. parvula_ and lowest in _S. mutans_. CHX DG induced lower viability on both alloys in _V. parvula_ and _S. mutans_, whereas in _A. actinomycetemcomitans_, it was only on NiTi, with a large effect size (\(p\leq 0.010\); r = 0.841-0.958). There were significant differences in the viability of the control on SS in the order _V. parvula_ \(>\)_S. oralis_ \(>\)_A. actinomycetemcomitans_ \(=\)_S. mutans_ (\(p<0.001\)). In the NiTi control, there were significant differences in viability in the order of _V. parvula_ \(>\)_A. actinomycetemcomitans_ \(>\)_S. _mutans_ (\(p<0.001\)). In both alloys, significant differences in the viability of the CHX DG were observed in the following order: _V. parvula_ \(>\)_A. actinomycetemcomitans_ \(>\)_S. _mutans_ \(=\)_S. oralis_ (\(p<0.001\)).  
Figure 2: Comparison of bacterial adhesion to alloys. Columns show mean values with 95% confidence intervals. Asterisks indicate significant differences between alloys for the same bacterium, and horizontal lines connect bacteria with a significant difference within the same alloy.  
Figure 4: Representative images of bacteria adhering within 4 h to NiTi and SS wires with and without CHX treatment. Biofilms were stained with the LIVE/DEAD Sustainability Kit (FilmTracerTM), LIVE/DEAD® Biofilm Viability Kit, Invitrogen). Green cells represent viable, live bacteria, while red cells represent dead bacteria.  
Figure 3: Comparison of control adhesion and antiseptic treatment. Average column values are with 95% confidence intervals. Significant differences between alloys for the same bacteria and antiseptic are marked with an asterisk. Horizontal lines connect antiseptics that show a significant difference between the same alloy and bacteria.