## 3 Results
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Extracts** & \multicolumn{2}{c}{**1 st Day**} & \multicolumn{2}{c}{**2 nd Day**} & \multicolumn{2}{c}{**3 rd Day**} \\ \cline{2-7}
**Concentration** & **MN \(\pm\) SD** & **GI [\%]** & **MN \(\pm\) SD** & **GI [\%]** & **MN \(\pm\) SD** & **GI [\%]** \\ \hline \hline Ferulic acid & & & & & & \\ control & 3.17 \(\pm\) 1.01 a & 0 & 2.72 \(\pm\) 1.73 a & 0 & 2.94 \(\pm\) 1.16 a & 0 \\
0.05 mg/mL & 2.06 \(\pm\) 1.47 b & 5.07 & 2.44 \(\pm\) 0.96 a & 10.30 & 2.71 \(\pm\) 1.56 a & 7.83 \\
0.1 mg/mL & 1.56 \(\pm\) 0.76 b & 28.12 & 2.28 \(\pm\) 1.27 a & 16.18 & 2.44 \(\pm\) 2.00 a & 17.01 \\
0.2 mg/mL & 1.94 \(\pm\) 0.85 b & 10.60 & 1.88 \(\pm\) 0.68 a & 30.89 & 2.00 \(\pm\) 1.41 a & 31.98 \\ p-comunic acid & & & & & \\ control & 3.17 \(\pm\) 1.01 a & 0 & 2.72 \(\pm\) 1.73 a & 0 & 2.94 \(\pm\) 1.16 a & 0 \\
0.05 mg/mL & 1.72 \(\pm\) 1.04 b & 45.75 & 2.28 \(\pm\) 1.18 a & 16.18 & 2.41 \(\pm\) 1.42 a & 18.03 \\
0.1 mg/mL & 1.67 \(\pm\) 1.45 b & 47.32 & 2.11 \(\pm\) 1.66 a & 22.43 & 2.24 \(\pm\) 1.00 a & 25.81 \\
0.2 mg/mL & 1.50 \(\pm\) 1.21 b & 52.69 & 2.00 \(\pm\) 1.20 a & 26.48 & 2.17\(\pm\) 1.26 a & 26.20 \\ \hline \hline \end{tabular}
\end{table} 
Table 6: Effect of ferulic acid and \(p\)-comunic acid on inhibition of _Acanthamoeba_ trophozoites during three days of treatment. \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Extracts** & \multicolumn{2}{c}{**1 st Day**} & \multicolumn{2}{c}{**2 nd Day**} & \multicolumn{2}{c}{**3 rd Day**} \\ \cline{2-7}
**Concentration** & **MN \(\pm\) SD** & **\% GS/GI** & **MN \(\pm\) SD** & **\% GS/GI** & **MN \(\pm\) SD** & **GI [\%]** \\ \hline \hline Rosmarinic acid & & & & & \\ control & 4.28 \(\pm\) 2.02 ab & 0 & 8.66 \(\pm\) 2.36 ab & 0 & 10.31 \(\pm\) 2.68 a & 0 \\
0.01 mg/mL & 5.61 \(\pm\) 2.03 a & 31.07 **ST** & 6.05 \(\pm\) 2.39 c & 31.34 IN & 9.33 \(\pm\) 2.81 b & 9.51 IN \\
0.05 mg/mL & 5.50 \(\pm\) 2.01 a & 28.50 **ST** & 7.33 \(\pm\) 2.49 bc & 15.36 IN & 8.19 \(\pm\) 2.92 b & 20.56 IN \\
0.1 mg/mL & 3.67 \(\pm\) 1.25 b & 14.26 IN & 9.44 \(\pm\) 2.33 a & 9.00 **ST** & 10.61 \(\pm\) 2.75 a & 2.91 **ST** \\ Chlorogenic acid & & & & & \\ control & 5.28 \(\pm\) 2.02 a & 0 & 8.66 \(\pm\) 2.36 a & 0 & 10.31 \(\pm\) 2.68 a & 0 \\
0.01 mg/mL & 4.44 \(\pm\) 1.50 b & 15.91 & 5.33 \(\pm\) 2.05 b & 38.46 & 8.71 \(\pm\) 1.66 a & 15.52 \\
0.05 mg/mL & 4.72 \(\pm\) 1.91 b & 10.61 & 5.83 \(\pm\) 1.64 b & 32.68 & 8.35 \(\pm\) 2.54 a & 19.02 \\
0.1 mg/mL & 4.83 \(\pm\) 1.89 b & 8.53 & 6.15 \(\pm\) 1.16 b & 28.99 & 9.00 \(\pm\) 3.21 a & 22.71 \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Effect of rosmarinic acid and chlorogenic acid on inhibition of _Acanthamoeba_ trophozoites during three days of treatment. \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Extracts** & \multicolumn{2}{c}{**1 st Day**} & \multicolumn{2}{c}{**2 nd Day**} & \multicolumn{2}{c}{**3 rd Day**} \\ \cline{2-7}
**Concentration** & **MN \(\pm\) SD** & **\% GS** & **MN \(\pm\) SD** & **\% GS** & **MN \(\pm\) SD** & **\% GS** \\ \hline \hline control & 6.27 \(\pm\) 2.43 a & 0 & 8.59 \(\pm\) 2.43 b & 0 & 18.08 \(\pm\) 2.75 b & 0 \\
1 mg/mL & 6.35 \(\pm\) 2.19 a & 1.28 & 10.33 \(\pm\) 1.63 a & 20.26 & 19.63 \(\pm\) 4.53 b & 8.57 \\
5 mg/mL & 6.57 \(\pm\) 1.40 a & 4.78 & 10.76 \(\pm\) 1.70 a & 25.26 & 28.60 \(\pm\) 6.86 a & 58.19 \\
10 mg/mL & 6.92 \(\pm\) 1.69 a & 10.37 & 9.89 \(\pm\) 2.35 ab & 15.13 & 28.81 \(\pm\) 5.60 aa & 59.35 \\ \hline \hline \end{tabular}
\end{table} 
Table 5: Effect of extract from _Eryngium planum_ callus culture on inhibition of _Acanthamoeba_ trophozoites during three days of treatment. Chlorogenic acid exhibits a stronger inhibitory effect than rosmarinic acid, particularly on day 2, where inhibition increases significantly before gradually decreasing. Rosmarinic acid's effectiveness declines over time, and at higher concentrations (0.1 mg/mL), it even leads to growth stimulation, indicating a possible hormetic effect (low doses inhibit, while higher doses promote growth).