## 3 Results
### Identification of Differentially Expressed Genes and Gene Ontology Analyses
\begin{table}
\begin{tabular}{c c c c} \hline \hline
**Gene Name** & **Fold Change** & **Description** & **Related Function** \\ \hline AREG & 1172.7 & Ampliregulin & Cell differentiation, cell proliferation \\ SMOC1 & 1161.3 & SPARC-related modular calcium & Extracellular matrix \\  & & binding 1 & Cell migration, extracellular matrix \\ MMP1 & 829.2 & Matrix metalloproteinase 1 & Cell migration, extracellular matrix \\ STC1 & 791.3 & Stamnicolain 1 & Cell differentiation, cell proliferation \\ MMP10 & 349.8 & Matrix metalloproteinase 10 & Extracellular matrix \\ PGF & 341.9 & Placental growth factor & Angiogenesis \\ PTGS2 & 195.6 & Prostaglandin-endoperoxide & Angiogenesis, cell differentiation, \\ COL5A3 & 173.4 & Collagen type V alpha 3 & Extracellular matrix \\ IL24 & 156.0 & Interleukin 24 & Apoptotic process, cell death \\ FRZB & 119.4 & Frizzled-related protein & Cell differentiation, cell proliferation, inflammatory response \\ SRP2 & 112.8 & Bone morphogenetic protein 2 & Cell differentiation, cell proliferation, inflammatory response \\  & & Angiogenesis, apoptotic process, cell death, cell differentiation, cell proliferation, extracellular matrix \\  & & Immune response \\ FGL2 & 98.6 & Fibrinogen-like 2 & Apoptotic process, cell death \\ IER3 & 97.3 & Immediate early response 3 & Apoptotic process, cell death \\ TFIP2 & 94.9 & Tissue factor pathway inhibitor 2 & Extracellular matrix \\ DPT & 83.6 & Dermatopontin & Extracellular matrix \\ NR4A2 & 82.5 & Nuclear receptor subfamily 4 group & Cell differentiation, cell migration \\  & & A member 2 & Cell differentiation, extracellular matrix \\ TNC & 82.1 & Tenascin C & Cell differentiation, extracellular matrix \\ NPNT & 73.8 & Nephronectin & Cell differentiation, extracellular matrix \\ NDP & 64.7 & Norrie disease (pseudologioma) & Cell proliferation, extracellular matrix \\ ACKR4 & 64.5 & Atypical chemokine receptor 4 & Immune response \\ IL18R1 & 63.5 & Interleukin 18 receptor 1 & Cell differentiation, immune response \\ MMP8 & 62.5 & Matrix metalloproteinases 8 & Extracellular matrix \\ SPRY1 & 61.6 & Sprouty RTK signaling antagonist 1 & Cell cycle, cell differentiation \\ RGS2 & 61.4 & Regulator of G-protein signaling 2 & Cell cycle, cell differentiation \\ IL1R1 & 60.3 & Interleukin 1 receptor type 1 & Immune response \\ Prostaglandin-endeoroxide & Inflammatory response \\ PTGS1 & 60.3 & synthase 1 & Inflammatory response \\ SPON1 & 53.6 & Spondin 1 & Extracellular matrix \\ EDNRB & 52.4 & Endothelial receptor type B & Cell differentiation, cell migration \\ PECAM1 & 49.3 & Platelet and endothelial cell & Angiogenesis, cell differentiation, cell migration \\ IL1 RN & 46.1 & Interleukin 1 receptor antagonist & Immune response, inflammatory response \\ PRDM1 & 44.6 & PR domain 1 & Cell differentiation, cell proliferation, immune response \\ CHST2 & 42.1 & Carbohydrate sulfotransferase 2 & Inflammatory response \\ APOE & 40.0 & Apolipoprotein E & Cell differentiation, extracellular matrix \\ NOX4 & 38.8 & NADPH oxidase 4 & Cell differentiation, inflammatory response \\ SNAP25 & 38.2 & Synaptosome-associated protein 25 & Cell differentiation, immune response \\ TNN & 36.8 & Tenascin N & Cell differentiation, extracellular matrix \\ VAV3 & 34.8 & VAV guanine nucleotide exchange & Angiogenesis, cell migration \\ BCL2A1 & 31.5 & BCL2-related protein A1 & Apoptotic process, cell death, cell differentiation, cell migration, extracellular matrix, immune response \\ JUP & 30.3 & Junction plakoglobin & Cell differentiation, cell differentiation \\ AKR1C1 & 30.3 & Aldo-keto reductase family 1, member C1 & Cell differentiation \\ NRN1 & 29.8 & Neuritin 1 & Cell differentiation \\ ITGA2 & 28.6 & Integrin subunit alpha 2 & Cell differentiation, cell migration \\ SLC1A3 & 28.3 & Solute carrier family 1 member 3 & Cell differentiation \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Genes upregulated more than 10-fold in 3D- compared to 2D-cultured sPDLSCs.