## 2 Results
### Transcriptome Analysis  
Transcriptome analysis of differentially expressed genes (DEGs) between rice-blast-inoculated WT and _OsVTC1-1_ RI1-2 plants was analyzed at 0 and 24 hpi. The RNA-seq data showed that the raw sequence reads of each sample ranged from 43,787,008 to 64,022,678. Reads were filtered and mapped to a reference sequence and more than 90% of the reads aligned successfully with the reference genome (Table S2). In total, there are 9075 DEGs in WT and RI1-2 represented by 4164 up-regulated DEGs and 4911 down-regulated DEGs. Among these DEGs, 675 up-regulated DEGs and 966 down-regulated DEGs were only presented in WT, while 932 up-regulated DEGs and 914 down-regulated DEGs were only presented in RI1-2. Additionally, 2557 up-regulated DEGs and 3031 down-regulated DEGs overlapped both in WT and RI1-2 (Figure 6a,b). Principal component analysis (PCA) was performed to verify the similarity between three replicates based on the fragments per kilobase of transcript per million mapped read (FPKM) values transformed into log10 (FPKM + 1). The data of 100 common genes from the WT and _OsVTC1-1_ RI1-2 line was clustered closely at 0 and 24 hpi and was grouped by time point on the PCA plot (Figure S1). The gene expression levels of 100 common genes were similar among three replicates based on log10 (FPKM + 1) values for WT and _OsVTC1-1_ RI1-2 lines at 0 and 24 hpi (Figure S2, Table S3). Table 1 lists the top 20 up- and down-regulated DEGs found only in the _OsVTC1-1_ RI1-2 line ranked by log2 FC value. Several defense-related genes were up-regulated at 24 hpi in the _OsVTC1-1_ RI1-2 line, including _germin-like protein 8-10, peroxidase, cytochrome P450 family, chitinase-4 or pathogenesis-related (PR)-3, glycoside hydrolase, chymotrypsin protease inhibitor, germin-like protein 8-5, terpene synthase_, and _germin-like protein 8-7_. Several DEGs were down-regulated in _OsVTC1-1_ RI1-2, including _S-like ribonuclease, tonoplast membrane integral protein, UDP-glucuronosyl/UDP-glucosidasetransferase, aldo/keto reductase_, and _flavin-containing monooxygenase_ (Table 1). Gene ontology (GO) enrichment analysis was used to classify the 932 up-regulated DEGs between 0 and 24 hpi in the _OsVTC1-1_ RI1-2 line (Table S4). DEGs were divided into three major categories: biological process, cellular component, and molecular function (Figure 6c). Among the three groups, DEGs were further subdivided into 19 subgroups of biological process, 17 subgroups of cellular component, and 9 subgroups of molecular function. The three most abundant biological process subgroups were cellular process (331 DEGs), metabolic process (259 DEGs), and biological regulation (142 DEGs). In addition, 10 DEGs in regulation of defense response subgroup were also enriched in biological process, including _LRR receptor kinase, protein TIFY, WRKY transcription factor WRKY62, protein negative regulator of resistance_, and _NRR repressor_ (Figure 6d). A major subgroup of cellular component is cellular anatomical entity (460 DEGs), followed by intracellular anatomical structure (287 DEGs) and intracellular organelle (242 DEGs). In terms of molecular function, the three most significant subgroups were binding (311 DEGs), catalytic activity (284 DEGs), and organic cyclic compound binding (213 DEGs) (Figure 6c). An analysis of plant reactome pathways was conducted to visualize up-regulated DEGs between 0 and 24 hpi with rice blast fungus in the _OsVTC1-1_ RI1-2 line. A total of 932 DEGs was identified in 82 plant reactome pathways (Table S5). The top 10 enriched pathways include metabolism and regulation; hormone signaling, transport, and metabolism; carbohydrate metabolism; growth and developmental processes; reproductive structure development; seed development; seed size regulation; amino acid metabolism; jasmonic acid signaling; and salicylic acid signaling (Figure S3a). Additionally, a number of DEGs was also assigned to enzymes involved in the synthesis and signaling of hormones, including SA and JA (Figure 6e).