## 4 Discussion  
This research investigates the effectiveness of inactivating larval chironoid, an aquatic insect commonly found in water treatment processes, specifically in coagulation and chlorination units. The study aims to determine the most effective approach for inactivating the larvae by observing their behavior when exposed to coagulants and chlorine. The results show that the behavior of the larvae changes when subjected to different doses of coagulants and chlorine over a period of one hour to five days. Larvae exhibit different behaviors, such as looping and crawling. After being exposed to high doses of chemicals, some of the larvae die. Similar results indicated that the population of larval chironomids when exposed to a polluted environment, exhibits specific responses in the form of changes in larval behavior and morphology [15; 16; 17]. The effects of the pesticide as a pollutant also change the morphology and behavior of chironomus riparius larvae. Since larvae are exposed to pollutants, high doses of which can change their behavior, extended exposure can also result in morphological changes in the larvae. These changes may manifest as a reduction in size, as well as changes in the shape of the head and thorax. Additionally, changes in larval behavior include decreased movement and changes in feeding behavior [18]. The effectiveness of coagulation in inactivating chironoid larvae in water treatment processes has been the focus of recent research. The results of this research show that although coagulation can lead to a significant reduction in the number of larvae present in water, the death of the larvae may not occur immediately. Even up to five days after exposure to the coagulant, the larvae may exhibit normal activities, such as looping and crawling, and may even actively cover their bodies with floc. This study confirmed that the death of the larvae can be visually determined by their inability to cover their bodies with floc. However, it is also possible that some larvae may have died even if they were covered by floc. The study by [PERSON] et al. [3] further supported the effectiveness of coagulation in removing chironomid larvae, with 75.5% of the larvae being removed. The death of the larvae was also attributed to the fact that the first instar larvae, which resembles a planktonic form, can easily settle together with the floc formed during coagulation. This highlights the need for more research to determine the underlying mechanisms involved in the inactivation of chironomid larvae through coagulation. This study revealed some interesting findings on the development of chironomid larvae exposed to the coagulation process. The observation that larvae continued to develop even after the settling process is particularly significant as it suggests that the coagulation process may not be effective in completely inhibiting the growth and development of chironomid larvae. It is possible that some larvae are able to survive and thrive in the presence of coagulants. Moreover, the fact that larvae in samples with high turbidity exhibited more pronounced development and some even reached maturity highlights the importance of considering other factors, such as water quality and environmental conditions, in the control of chironomid larvae in water treatment processes [19]. Our study also provides useful insights into the potential limitations of the coagulation process in controlling chironomid larvae in water treatment. The presence of larval skin on the fifth day of observation indicates that chironomid larvae may be more resilient than previously thought and that other treatment methods may need to be explored to ensure the complete removal of these organisms from water. Moreover, based on these findings, it is recommended that the floc produced during the coagulation process should be cleanedticulously. This is because the floc may harbor larvae that have the potential to persist and continue their development.