## 3 Results and Discussions
### HPLC-MS Analysis of Phenolic Compounds
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Validation** & **Calibration Equation** & **R2** & \begin{tabular}{c} **LOD** \\ **\#g/g** \\ \end{tabular} & **LOQ\#g/g** & \begin{tabular}{c} **Precision (RSD, \%)** \\ **Intra-Day** \\ \end{tabular} & \
\begin{tabular}{c} **Repetability** \\ **(RSD, \%)** \\ \end{tabular} \\ \hline Hidrovic- &
\begin{tabular}{c} Y = 11307X + 4493 \\ \end{tabular} & 0.992 & 10.40 & 31.52 & 3.15 & 2.85 & 4.31 \\ \hline Epigallocatechin & Y = 11198X + 2849 & 0.993 & 9.65 & 29.24 & 2.49 & 2.62 & 3.56 \\ \hline Epigallocatechingallat & Y = 3240X \(-\) 3511 & 0.990 & 16.84 & 51.03 & 2.36 & 4.1 & 2.82 \\ \hline Gallocatedachingallat & Y = 8671X + 2133 & 0.994 & 18.21 & 55.18 & 1.89 & 2.36 & 4.27 \\ \hline Coumestrol & Y = 4735X \(-\) 2771 & 0.995 & 0.82 & 2.48 & 2.23 & 3.04 & 3.95 \\ \hline Catechin & Y = 1435X + 305 & 0.993 & 0.35 & 1.06 & 2.81 & 5.01 & 3.97 \\ \hline Secolariciresinol & Y = 1001X \(-\) 261 & 0.998 & 1.65 & 5.00 & 2.65 & 3.25 & 4.04 \\ \hline Orientin & Y = 5100X \(-\) 4382 & 0.998 & 2.31 & 7.00 & 3.45 & 3.89 & 5.01 \\ \hline Isoorientin & Y = 1294X + 525 & 0.991 & 1.15 & 3.48 & 4.25 & 3.87 & 3.26 \\ \hline Vitexin & Y = 7555X + 2105 & 0.998 & 2.47 & 7.48 & 3.29 & 4.32 & 3.57 \\ \hline Rutin & Y = 3022X + 156 & 0.996 & 11.02 & 33.39 & 2.47 & 2.65 & 2.12 \\ \hline Isorhamenti glucuronid & Y = 43279X \(-\) 1900 & 0.996 & 3.45 & 10.45 & 2.43 & 3.16 & 5.04 \\ \hline Quercetin arabinosid & Y = 74639X + 2708 & 0.994 & 8.31 & 25.18 & 1.26 & 2.08 & 3.44 \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Analytical performances of the method used for the quantification of individual flavonoid compounds in buckwheat seeds, and after 3 and 7 days of sprouting. In Table 4 and Figure 3 are quantified the flavonoids present in buckwheat achenes, and in sprouted buckwheat after three and seven days. \begin{table}
\begin{tabular}{c c c c c} \hline \multirow{2}{*}{**No.**} & \multirow{2}{*}{**Compound**} & \multicolumn{2}{c}{**Buckwheat’s Achenes,**} & \multicolumn{2}{c}{**Buckwheat’s Achenes after 3**} & \multicolumn{2}{c}{**Buckwheat’s Achenes after 7**} \\  & \multicolumn{2}{c}{\(\mu\)g dwb} & \multicolumn{2}{c}{**Days of Sprouting, \(\mu\)g/dwb**} & \multicolumn{2}{c}{**Days of Sprouting, \(\mu\)g/g dwb**} \\ \hline
1 & Hydroxysecoisolaricinesinol & 886.70 \(\pm\) 19.02 & 604.52 \(\pm\) 25.01 & 145.52 \(\pm\) 4.88 \\ \hline
2 & EGC & 131.58 \(\pm\) 15.22 & 28.22 \(\pm\) 6.22 & 85.55 \(\pm\) 5.33 \\ \hline
3 & EGCG & 44.20 \(\pm\) 5.24 & 88.40 \(\pm\) 10.39 & n.d. \\ \hline
4 & GCG & 56.13 \(\pm\) 6.66 & 32.47 \(\pm\) 8.77 & n.d. \\ \hline
5 & Coumestrol & 34.47 \(\pm\) 4.87 & 395.23 \(\pm\) 25.1 & 917.71 \(\pm\) 15.74 \\ \hline
6 & Catechin & 108.97 \(\pm\) 5.21 & 139.49 \(\pm\) 7.46 & 170 \(\pm\) 8.54 \\ \hline
7 & Secoisolaricinesinol & 323.51 \(\pm\) 6.98 & 229.67 \(\pm\) 10.85 & n.d. \\ \hline
8 & Orientin & 178.93 \(\pm\) 7.09 & 230.28 \(\pm\) 9.56 & 2361.50 \(\pm\) 24.99 \\ \hline
9 & Isoorientin & 114.28 \(\pm\) 8.04 & 342.86 \(\pm\) 9.34 & 1475.85 \(\pm\) 35.88 \\ \hline
10 & Vitexin & 155.74 \(\pm\) 6.79 & 173.22 \(\pm\) 12.40 & 5115.31 \(\pm\) 37.65 \\ \hline
11 & Rutin & 37.52 \(\pm\) 3.77 & 420.47 \(\pm\) 13.56 & 558.47 \(\pm\) 8.22 \\ \hline
12 & Isohametin & 39.23 \(\pm\) 6.85 & 326.15 \(\pm\) 9.91 & 385.17 \(\pm\) 7.35 \\ \hline
13 & Quercetin arabinosid & 299.19 \(\pm\) 24.02 & 713.36 \(\pm\) 27.44 & 885.17 \(\pm\) 16.43 \\ \hline
14 & Quercetin rannosid (Quecitrin) & 227.04 \(\pm\) 16.54 & 547.38 \(\pm\) 12.74 & n.d. \\ \hline
15 & Quercetin & 73.37 \(\pm\) 3.63 & 173.31 \(\pm\) 8.87 & 279.75 \(\pm\) 9.58 \\ \hline
16 & Isohametin & 130.83 \(\pm\) 5.44 & 183.03 \(\pm\) 11.57 & 618.17 \(\pm\) 8.33 \\ \hline
17 & Luteolin & 52.02 \(\pm\) 3.44 & 260.13 \(\pm\) 8.89 & 184.91 \(\pm\) 4.75 \\ \hline
18 & Apigenin & 54.58 \(\pm\) 3.98 & 111.21 \(\pm\) 12.47 & n.d. \\ \hline \end{tabular}
\end{table} 
Table 4: The concentrations of flavonoids extracted, in \(\mu\)g/g dw, in buckwheat achenes, and buckwheat achenes after 3 and 7 days of sprouting.