## 1 Introduction
To this end, this work showed the comparison between the European Cooperation in Science and Technology Reference Microplasma Jet (COST-Jet) [25] and the nspDBD [26] from both chemical composition and biological response perspective. Further, modifications on the cysteine-containing protein glutathione were compared for an attempt to establish correlations with cellular outcomes. The plasma discharge components that contribute to biological responses following exposure vary in magnitude between the two devices. The COST-Jet generates myriad RONS in the active plasma, influenced by admixtures and absorbed power, that undergo complex reactions with molecules in the ambient air [11; 25; 27; 28]. Short-lived species, such as a nitric oxide (NO), hydroxide radical (OH), and atomic oxygen (O), and long-lived species are transported by gas flow and solvate into the liquid interface [15; 21; 29; 30]. While the UV produced in the discharge radiates towards the liquid interface and may influence the treated targets, the electric field does not affect the targets since it is perpendicular to the gas flow (Figure 1). In contrast, the nspDBD produces short and long-lived RONS at the liquid interface. The charged species are driven by the electric field to the liquid interface, whereas the neutral species have diffusion dominated transport and solvate as governed by Henry's law [31]. The UV and electric field components of the nspDBD are directed towards the liquid interface. These nspDBD components individually influence the cellular response at different magnitudes [26]. Therefore, the contribution of any plasma component may significantly influence the cellular response in one treatment approach and be insignificant in another. To compare how these components may affect different plasma treatment approaches, where the components have varying influence on the overall cellular response, the cell's biological outcome must be comparable. This study focused on determining if correlations could be established between biological responses of cells to plasma treatment and the modifications to GSH structure caused by plasma. [PERSON] et al. could show that plasma treatment modified GSH, and the magnitude of these modifications increased with plasma treatment time [23]. We expanded this work by investigating the relationship between observed biological outcomes in Jurkat T lymphocytes and modifications to GSH from two different plasma sources. First, we demonstrated that these two plasma sources could be operated at parameters where comparable reductions in Jurkat T lymphocyte viability were achieved. This served as a benchmark for further chemical and biological comparisons of the two plasma sources. Next, we showed that the levels of mitochondrial superoxide (O2-) production and translocation of calreticulin to the outer surface of the cell membrane (ecto-CRT) were comparable between the two sources at these plasma treatment parameters. The measured concentrations of long-lived RONS were higher in the medium than in deionized water. This indicated that reactions with the medium components produced more RONS in the liquid than without organic compounds. Even with higher concentrations in the medium, the reactions between plasma-generated RONS and the medium components reduced the toxicity observed from H2O2 alone. We showed that exogenously added long-lived species alone were not responsible for inducing these biological changes. Finally, we evaluated GSH modifications as a potential, scalable marker for correlations between observed biological responses and plasma parameters. Our results showed that changes in a marker, such as GSH, could elucidate the plasma-generated chemistry unique to each source and might correlate with potential biological applications for the plasma source. While the modifications indicated the presence of specific RONS, the biological implications of these RONS were dependent on both their concentration and the cell type.