## 3 Results  
The analysis of \(\alpha\)-diversity coefficients demonstrated that _S. oryzae_ (Figure 1a) had a three-fold higher value of the dominance index, as well as greater diversity and uniformity, which was due to a much larger number of occasional taxa compared to _R.__dominica_ (Figure 1b). Based on the barcoding analysis of the full 16S rRNA region, six types of bacteria colonizing the microbiome of _S. oryzae_ were determined with their proportion in the bacterial community of at least 0.5%. On analyzing the general structure of the identified microbiota of the adult rice weevil form, the presence of two phyla with their proportion in the bacterial community at >0.5%, Proteobacteria 97.9% and Firmicutes 1.3%, was observed. Proteobacteria were almost completely dominated by the Pectobacteriaceae family with the participation of Enterobacteriaceae, while the Firmicutes structure included many species of Staphylococcus to with a very low abundance. The results of the predicted species colonizing the insect bacteria demonstrated the complete predominance of _Sodalis pierantonius_ and the relatively clear influence of _Escherichia coli_ and the presence of _Sodalis pracapativisus_. It is surprising that it is _E. coli_ bacteria that are eight times more numerous than _Enterobacter_ spp., which form the typical insect gut microbiota (Figure 1a, Table 1). Figure 1: Results of nanopore 16S rRNA sequencing for the bacterial microbiome inhabiting the digestive system of (**a**) _S. oryzae_ and (**b**) _R. domítica._  
The results of the nanopore sequencing analysis for _R. dominica_ showed a much lower dominance of bacteriophage taxa than in the case of _S. oryzae_. The phylum with a high proportion in the bacterial community includes Bacteroidetes, which is more than half of the bacteriophage of the Flavobacterialies order (\(\sim\)50% of the bacteriophage) consisting mainly of _Sulcia_ sp. (38%), _Blattabacterium_ sp., and _Walczuchela_ sp. and the order Cytophagales with a small number of bacterial genera, represented most frequently by the genus _Anoebophilus_ (4.0%). The phylum Firmicutes was also abundant with the proportion in the bacterial community of about 25%, almost completely dominated by Clostridiales with the _Cellulosiliticum_ (-15%) and _Ruminococcus_ (-10%) genera, and the order Bacillales (2.0%) represented mainly by bacteria of the genus _Staphylococcus_. The phylum Proteobacteria accounted for almost 7% of the bacterial community and was represented by _Brevundomonas_ sp. (4.7%) and many less abundant taxa. The structure of the dominance classes of _R. dominica_ differed from those of _S. oryzae_. _R. dominica_ had equominant species _Sulcia muelleri_; however, also dominant were representatives of _Ruminococcus_ sp. and _Walczuchela monophlebidarum_, as well as two subdominants and three rare species, two of which belonged to _Blattabacterium_ sp. (Table 1, Figure 1b). The results of the microbiological analysis conducted for individuals feeding on feed with antibiotics showed a similar reduction in the number of bacteria in both species of insects. On the other hand, administered feed with antibiotics showed the stability of the microbiome structure and abundance of _R. dominica_, while the degeneration of the microbial community structure in the case of _S. oryzae_ occurred in part of the variant. A significant reduction in the abundance of microorganisms was observed on the third feeding day at a dose of 30 mg\(\cdot\)g\({}^{-1}\) and higher (Figure 2).