## Abbreviations  
* [1] [PERSON]; [PERSON] Nmr studies on the presence of domain structures in polysaccharides. _Biopolymers_**1987**, _26_, 849-861. [CrossRef]
* [2] [PERSON]; [PERSON]; [PERSON] Comparison of celery (_Apium graveolus_ L.) collenchyma and parenchyma cell wall polysaccharides enabled by solid-state \({}^{13}\)C NMR. _Carbolohydr. Res._**2015**, _420_, 51-57. [CrossRef] [PubMed]
* [3] [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON] Charting the solid-state NMR signals of polysaccharides: A database-driven roadmap. _Magn. Reson. Chem._**2024**, _62_, 298-309. [CrossRef] [PubMed]
* [4] [PERSON]; [PERSON]; [PERSON]; [PERSON] Positional specificity of acetylxylan esterases on natural polysaccharide: An NMR study. _Biochim. Biophys. Acta Gen. subj._**2013**, _1830_, 3365-3372. [CrossRef] [PubMed] \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Polysaccharide Type** & **Structural Features** & **Recommended Techniques** & **Rationale** \\ \hline Linear homopolysaccharides (e.g., cellulose) & Simple repeats, rigid chains & Solution: 1D \({}^{13}\)C NMR; & 1D NMR confirms composition; CP/MAS \\  & & solid: CP/MAS & distinguishes \\  & & & crystalline domains \\ Branched heteropoly- & Complex side chains, & HSQC (linkages) + HMBC & Multidimensional approach \\ saccharides (e.g., pectin) & acidic groups & (sequence) + NOESY & needed for branching and \\  & & (conformation) & charge effects \\ Microbial EPS & High heterogeneity, modified & HSQC/TOCSY + DOSY; & Combination addresses \\  & groups (e.g., sulfation) & solid: DNP-NMR & structural diversity; DNP \\  & & (low-abundance) & enhances sensitivity \\ Food matrix polysaccharides & Multi-component, & DOSY + \({}^{1}\)H NMR & DOSY separates components; \\  & dynamic interactions & metabolomics; Solid: & HETCOR reveals hydration/ \\  & & HETCOR & protein interactions \\ Biofilm polysaccharides & Semi-rigid, & Solution: NOESY; solid: & DNP solves sensitivity issues; \\  & environment-sensitive & DNP-NMR + \({}^{1}\)H-detection & NOESY elucidates \\  & & biofilm formation \\ \hline \hline \end{tabular}
\end{table} 
Table 5: Recommended NMR techniques by polysaccharide type. [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON] Polysaccharide assemblies in fungal and plant cell walls explored by solid-state NMR. _Structure_**2023**, _31_, 1375-1385. [CrossRef]
* _[PERSON] et al._ (2025) [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON] [PERSON] Comparative analysis of polysaccharide and cell wall structure in _Aspergillus nilulans_ and _Aspergillus fumigatus_ by solid-state NMR. _Carbolhydr. Polym._**2025**, _348_, 122907. [CrossRef]
* [PERSON] (1983) [PERSON] Accurate referencing of \({}^{13}\)C NMR spectra from glycosaminoglycan and other polysaccharides in aqueous medium. _Org. Magn. Reson._**1983**, _21_, 67-70. [CrossRef]
* _[PERSON] et al._ (2021) [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON] A review of NMR analysis in polysaccharide structure and conformation: Progress, challenge and perspective. _Food Res. Int._**2021**, _143_, 110290. [CrossRef]
* _[PERSON] et al._ (2022) [PERSON]; [PERSON]; [PERSON] NMR spectroscopy for structural elucidation of sulfated polysaccharides from red seaweeds. _Int. J. Biol. Macromol._**2022**, _199_, 386-400. [CrossRef]
* _[PERSON] et al._ (2024) [PERSON]; [PERSON]; [PERSON] Molecular unraveling of polysaccharide digestion in wood-feeding termites: A solid-state NMR perspective. _Carbolydr. Polym._**2024**, _331_, 121843. [CrossRef]
* _[PERSON] et al._ (2010) [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON] Characterization of uniformly and atom-specifically \({}^{13}\)C-labeled heparin and heparan sulfate polysaccharide precursors using \({}^{13}\)C NMR spectroscopy and ESI mass spectrometry. _Carbolydr. Res._**2010**, _345_, 2228-2232.