## 2 Materials and Methods
### Inventory Analysis  
Comprehensive data on the cyanobacterium cultivation, harvesting and extraction, as well as the amount of chemicals (nutrients, solvents), water supply and waste volume, and electricity consumption, were obtained through laboratory-scale experiments [15,16]. Tables 1-3 include the Life Cycle Inventory considered for the laboratory-scale, demonstration-scale and industrial-scale processes. The electricity values considered for the inventory were based on the Portuguese mix (57% renewable) [39]. For the impact assessment, SimaPro 7.3 was used, and the Ecoinvent(r) database version 3.5 [40] was employed as a secondary data source. For the selection of characterisation factors required to estimate the environmental loads, the ReCiPe 2016 hierarchist Midpoint approach V1.03 World (2010) [41] was used, and a set of impact categories at the midpoint level was selected to report the environmental profiles. The following impacts have been considered: global warming (GW), stratospheric ozone depletion (SOD), terrestrial acidification (TA), freshwater eutrophication (FE), marine eutrophication (ME), terrestrial ecotoxicity (TET), freshwater ecotoxicity (FET), marine ecotoxicity (MET) human carcinogenic toxicity (HCT), human non-carcinogenic toxicity (HNCT), and fossil resource scarcity (FRS). \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Inputs from Technosphere** & & & & & \\ \hline _Materials_ & & & & & \\
**S1. Pre-inoculum** & & & & & \\ _Cleaning of the material_ & & & & & \\ NaCIO & 10 & g & Autoclave & 12 & kWh \\ Tap water & 400 & mL & & & \\ Water (deionised) & 200 & mL & Inchulator & 70 & kWh \\ _Medium preparation_ & & & & & White LED \\ BG11 solids 1  & 31.7 & g & Air pump & 8.4 & kWh \\ Water (deionised) & 2 & L & & **S2. Cultivation** & \\
**S2. Cultivation** & & & & & \\ _Cleaning of the material_ & & & & & \\ NaCIO & 100 & g & & & \\ Tap water & 4 & L & Inchulator & 218.4 & kWh \\ Water (deionised) & 2 & L & White LED & 22.4 & kWh \\ _Medium preparation_ & & & & Red LED & 9 & kWh \\ BG11 solids 2  & 285.3 & g & Air pump & 8.4 & kWh \\ Water (deionised) & 18 & L & & **S3. Harvesting** & & \\
**S5. Extraction I** & & & & & \\ Ethanol & 400 & mL & & **S4. Dewatering** & \\ Water (deionised) & 4 & mL & Freezer & 1.9 & kWh \\ NaCl & 0.1 & g & Freeze-drier & 48 & kWh \\
**S6. Extraction II** & & & & **S5. Extraction I** & \\ Water (deionised) & 400 & mL & Ohmic Heating & 0.1 & kWh \\  & & & & & Contrridge & 0.4 & kWh \\  & & & & Rotavapor & 1.1 & kWh \\  & & & & **S6. Extraction II** & \\  & & & & & Agitator & 2 & Wh \\  & & & & & Centrifuge & 0.4 & kWh \\  & & & & Freezer & 1.9 & kWh \\  & & & & & Freeze-drier & 48 & kWh \\  & & & & **S7. Residual biomass** & & \\  & & & & Dry oven & 16.8 & kWh \\ \hline \multicolumn{5}{c}{Outputs to technosphere} & & & \\ \hline Carotenoids’ extract & 10.8 & g & & & \\ Phycobiliproteins’ extract & 10 & g & & & \\ Residual biomass & 19.2 & g & & & \\ \hline Outputs to environment & & & & & \\ \hline Wastewater & 27 & L & & & \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Global inventory for the demonstration-scale production of _Cyanobium_ sp. (140 L) (functional unit: 1 batch). \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Inputs from Technosphere** & & & \\ \hline _Materials_ & & & \\
**S1. Pre-inoculum** & & & \\ \multicolumn{3}{c}{_Cleaning of the material_} & & \\ NaCIO & 7 & kg & Heat from steam & 36.6 & MJ \\ Tap water & 280 & L & _Inoculum_ & \\ Water (deionised) & 140 & L & Heater & 5.0 & kWh \\ _Medium preparation_ & & & Illumination & 0.3 & kWh \\ BG11 solids 2  & 221.9 & g & Air pump & 0.8 & kWh \\ Water (deionised) & 14 & L & **S2. Cultivation** & & \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Global inventory for the laboratory-scale production of _Cyanobium_ sp. (20 L) (functional unit: 1 batch).