## 3 Results and Discussion
### Simultaneous Quantification of the Eight Marker Constituents of ISYPT  
The HPLC method newly developed and verified in the present study was applied to the simultaneous analysis of eight marker constituents in lyophilized ISYPT. The constituents were quantified using a full scan from 190 to 400 nm with PDA detector, and each constituent was quantified based on its maximum UV absorption as follows: 250 nm for GCRZA and SZDRA, 275 nm for LQRTA and LQRTS, 280 nm for NRG, DDM, and PCR, and 325 nm for MBRSA. The eight representative marker constituents (MBRSA, LQRTA,  
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Analyte**} & **Linear Range** & **Regression Equation a ** & & \multirow{2}{*}{**\(r^{2}\)**} & **LOD b ** & **LOQ c ** \\  & **(\#g/mL)** & **\(y\)=ax+b** & & **(\#g/mL)** & **(\#g/mL)** \\ \hline MBRSA & 1.56–100.00 & \(y\) = 29,664.63\(x\) \(-\) 213.40 & 0.9999 & 0.40 & 1.21 \\ LQRTA & 0.78–50.00 & \(y\) = 14,626.08\(x\) \(+\) 664.05 & 0.9999 & 0.25 & 0.77 \\ LQRTS & 0.31–20.00 & \(y\) = 23,686.70\(x\) \(+\) 387.65 & 0.9999 & 0.10 & 0.30 \\ NRG & 0.78–50.00 & \(y\) = 17,632.59\(x\) \(+\) 1673.16 & 0.9999 & 0.11 & 0.33 \\ DDM & 0.47–30.00 & \(y\) = 19,190.72\(x\) \(+\) 964.97 & 0.9999 & 0.05 & 0.16 \\ PCR & 1.56–100.00 & \(y\) = 18,209.46\(x\) \(+\) 2968.69 & 0.9999 & 0.41 & 1.23 \\ GCRZA & 0.78–50.00 & \(y\) = 9833.62\(x\) \(+\) 170.54 & 0.9999 & 0.25 & 0.74 \\ SZDRA & 0.31–20.00 & \(y\) = 20,364.60\(x\) \(+\) 114.65 & 0.9999 & 0.03 & 0.08 \\ \hline \hline \end{tabular}
\end{table}
Table 1: Linear range, regression equation, coefficient of determination (\(r^{2}\)), limits of detection (LOD), and quantification (LOQ) for the simultaneous analysis of eight authentic standards by HPLC (\(n\) = 3).  
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Analyte**} & **Spiked Conc.** & **Found Conc.** & **Recovery (\%) a ** & **SD** & **RSD (\%) b ** \\  & 6.00 & 5.81 & 96.87 & 1.28 & 1.33 \\ MBRSA & 15.00 & 14.44 & 96.26 & 2.08 & 2.16 \\  & 30.00 & 29.75 & 99.18 & 1.32 & 1.33 \\ \hline \multirow{2}{*}{LQRTA} & 2.00 & 1.96 & 97.87 & 2.30 & 2.35 \\  & 5.00 & 5.12 & 102.38 & 1.93 & 1.89 \\  & 10.00 & 9.65 & 96.47 & 1.57 & 1.63 \\ \hline \multirow{2}{*}{LQRTS} & 1.00 & 0.99 & 99.09 & 1.80 & 1.82 \\  & 2.00 & 1.97 & 98.52 & 2.08 & 2.11 \\  & 4.00 & 4.06 & 101.60 & 1.52 & 1.50 \\ \hline \multirow{2}{*}{NRG} & 4.00 & 4.09 & 102.15 & 1.97 & 1.93 \\  & 10.00 & 9.90 & 99.05 & 1.54 & 1.55 \\  & 20.00 & 19.23 & 96.15 & 0.93 & 0.97 \\ \hline \multirow{2}{*}{DDM} & 1.00 & 0.98 & 98.02 & 1.92 & 1.96 \\  & 2.00 & 2.03 & 101.60 & 1.44 & 1.42 \\  & 4.00 & 3.97 & 99.24 & 0.66 & 0.66 \\ \hline \multirow{2}{*}{PCR} & 6.00 & 6.10 & 102.17 & 1.11 & 1.08 \\  & 15.00 & 15.00 & 99.98 & 0.80 & 0.80 \\  & 30.00 & 29.16 & 97.15 & 0.22 & 0.23 \\ \hline \multirow{2}{*}{GCRZA} & 2.00 & 2.03 & 101.69 & 1.00 & 0.99 \\  & 5.00 & 4.98 & 99.58 & 1.34 & 1.35 \\  & 10.00 & 9.62 & 96.17 & 0.69 & 0.72 \\ \hline \multirow{2}{*}{SZDRA} & 1.00 & 0.97 & 97.00 & 1.74 & 1.80 \\  & 2.00 & 1.91 & 95.67 & 0.67 & 0.70 \\ \cline{1-1}  & 4.00 & 3.94 & 98.51 & 0.61 & 0.62 \\ \hline \hline \end{tabular}
\end{table}
Table 2: Extraction recovery (%) test for the analytical method using eight authentic standards (\(n\) = 5).  
LQRTS, NRG, DDM, PCR, GCRZA, and SZDRA) were detected in the lyophilized ISYPT samples at concentrations ranging 0.09-5.95 mg/g (Table 4).