## 4 Conclusions  
The data analysis obtained in the experiments performed on two tumor cell lines with different localizations showed that the complexes studied with Al and Ga acted differently on the tumor processes. The results emphasized that the complexes with Al and Ga can act as cytostatic agents depending on the concentration and time of treatment, as well as the type and origin of the tumor cells. In conclusion, the hydrophilic complexes Al-5 HF-PMDACD1 and Ga-5 HF-PMDACD1 significantly affect LoVo colon tumor cells. Simultaneously, the hydrophobic complexes Al-5 HF-DPCNS2 and Ga-5 HF-DPCNS2 were the most effective on SKOV-3 tumor cells, the results for both cell lines being comparable with CisPt.  
Within the studies on target identification and structure optimization for increased cellular uptake, the possibility of entrapping the complexes in cyclodextrin-based nanosponges  
Figure 13: Effect of the analyzed compounds on the apoptotic process of the SKOV-3 ovarian cancer cells after (**a**) 24 h of treatment and (**b**) 48 h of treatment.  
or hydrophilic reticulate CD polymers is an actual and interesting approach. The inclusion complexes of Al-5-HF and Ga-5 HF with DPCNS and polyPMDACD were characterized. When comparing the cytostatic activity of Ga-5 HF and Al-5 HF and their prepared supramolecular structures, the Ga complex and its derivatives proved to be much more effective than the Al complex.  
The obtained data encourage the elaboration of further research directions of the study, implying the evaluation of cell cycle phases. The development of new molecular targets and the evaluation of their potential apoptotic effects through cellular and molecular mechanisms are considered future perspectives of the present research.  
The studied compounds may open new pathways for cancer treatment leading to improved therapeutic response in ovarian or colorectal cancer.  
The following supporting information can be downloaded at [[https://www.mdpi.com/article/10.3390/app14135441/s1](https://www.mdpi.com/article/10.3390/app14135441/s1)]([https://www.mdpi.com/article/10.3390/app14135441/s1](https://www.mdpi.com/article/10.3390/app14135441/s1)): Figures S1-S8: FT-IR spectra of Al-5 HF, Ga-5 HF, Al-5 HF-PMDACD1, Ga-5 HF-DPCNS1, Ga-5 HF-DPCNS2, respectively, and Ga-5 HF-DPCNS2.  
Conceptualization, C.R., A.A.O., M.M. and V.U.; methodology, C.R., M.M., A.A.O. and C.C.A.; software, C.R., M.M., A.A.O. and C.C.A.; validation, C.R., M.M. and C.C.A.; formal analysis, C.R., M.M., A.A.O. and C.C.A.; investigation, C.R., M.M., A.A.O. and C.C.A.; resources, C.R., M.M., V.U. and C.C.A.; data curation, V.U. and C.C.A.; writing--original draft preparation, C.R. and A.A.O.; writing--review and editing, C.R., M.M. and A.A.O.; visualization, V.U. and C.C.A.; supervision, V.U.; project administration, C.R.; funding acquisition, C.R. and A.A.O. All authors have read and agreed to the published version of the manuscript.  
This research received no external funding.  
Not applicable.  
The original contributions presented in the study are included in the article, further inquiries can be directed to the corresponding author.  
The publication of this paper was supported by the University of Medicine and Pharmacy Carol Davila, through the institutional program Publish not Perish.  
The authors declare no conflicts of interest.