## 3 Results
The suspension cultures exhibit little to no antimicrobial activity across all tested microorganisms. Gram-positive bacteria (_Staphylococcus_ species) are more sensitive to the extracts compared to Gram-negative bacteria and fungi. _Pseudomonas aeruginosa_ and _Candidida albicans_ are resistant to all the tested plant extracts. The _E. planum_ and _K. elatine_ callus cultures show moderate antibacterial potential, while the _Lychnis flos-cuculi_ extracts are mostly ineffective. The _E. planum_ suspension culture shows weak activity against _S. aureus_ (MIC = 100 mg/mL) but is ineffective against all other microbes. The _K. elatine_ and _L. flos-cuculi_ suspension cultures are ineffective (MIC > 100 mg/mL for all strains). The suspension cultures are consistently less effective than their callus counterparts. The plant extracts require much higher concentrations (\(\geq\)25 mg/mL) to have an antibacterial effect, making them far less potent than standard antibiotics. Further testing on purified bioactive compounds from _E. planum_ and _K. elatine_ is necessary to enhance antibacterial effects. Combination studies with standard antibiotics are needed to assess potential synergistic effects. \begin{table}
\begin{tabular}{c c c} \hline  & \(\Delta\)G (kcal/mol) & **Inhibitory Constant** \\ \hline Acteoside & \(-\)2.86 & 8.02 mM \\ Chlorogenic acid & \(-\)4.58 & 437.19 uM \\ \(p\)-coumaric acid & \(-\)4.33 & 667.05 uM \\ Ferulic acid & \(-\)3.80 & 1.64 mM \\ Rosmarinic acid & \(-\)4.58 & 483.33 uM \\ \hline \end{tabular}
\end{table} 
Table 10: Docking \(\Delta\)G (kcal/mol) and inhibitory constant scores of selected main compounds from cell biomass of _Erngium planum_, _Kickixa elatine_, and _Lychnis flos-cuculi_ on the active site of _Acanthamoeba_ profilin 1B protein (PDB 1 ACF). \begin{table}
\begin{tabular}{l c c c c c c c c} \hline  & \multicolumn{2}{c}{_S. aureus_ ATCC} & \multicolumn{2}{c}{_S. epidermidis_ ATCC} & \multicolumn{2}{c}{_P. aeruginosa_ ATCC} & \multicolumn{2}{c}{_C. albicans_ ATCC} \\
**Extract** & \multicolumn{2}{c}{**25923**} & \multicolumn{2}{c}{**35984**} & \multicolumn{2}{c}{**27853**} & \multicolumn{2}{c}{**10231**} \\  & **MIC** & **MBC** & **MIC** & **MBC** & **MIC** & **MBC** & **MIC** & **MFC** \\ \hline _Eryngium planning_ & & & & & & & & & \\ in vitro shoot culture & 12.5 & 50 & 12.5 & 25 & 100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 \\ callus culture & 50 & 100 & 50 & 100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 \\ cell suspension culture & 100 & 100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 \\ \hline _Kickxia elatine_ & & & & & & & & \\ in vitro shoot culture & 100 & 100 & 25 & 100 & 100 & 100 & \textgreater{}100 & \textgreater{}100 \\ callus culture & \textgreater{}100 & \textgreater{}100 & 25 & 100 & 100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 \\ cell suspension culture & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 \\ \hline _Lyclnis flos-cuculi_ & & & & & & & & \\ in vitro shoot culture & 25 & 50 & \textgreater{}100 & \textgreater{}100 & 100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 \\ callus culture & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 \\ cell suspension culture & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 & 100 & \textgreater{}100 & \textgreater{}100 & \textgreater{}100 \\ \hline Gentamycin & 0.125 & 0.25 & - & - & 0.125 & 0.125 & - & - \\ Nystatin & - & - & - & - & - & - & 0.125 & 0.25 \\ \hline \end{tabular}
\end{table}