## 3 Results
### Apoptosis Assay  
A study of apoptosis using a flow cytometer showed that fluoride caused a reduction in the number of viable cells by 9.1%. However, cell exposure to a SMF reduced fluoride-induced programmed cell death. This effect was stronger at the higher magnetic field intensities, i.e., the SMF with a 0.55 T flux density reduced the apoptosis process by 3.1% and the SMF with a 0.65 T flux density by 15.7%. (Figure 1).  
\begin{table}
\begin{tabular}{c c c c c c c} \hline  & **C** & **F** & **F + SMF1** & **F + SMF2** & **F + SMF3** & \({}^{*}p\) \\ \hline \multicolumn{8}{c}{**mRNA Copy Numbers/\(\upg\) RNA**} \\ \hline \(BAX\) & 162,150 \(\pm\) & 1,057,813 \(\pm\) & 260,906 \(\pm\) & 165,288 \(\pm\) & 315,338 \(\pm\) & NS \\ (BCL2 associated X, apoptosis regulator) & 56,390 & 1,343,514 & 90,299 & 55,281 & 278,153 & NS \\ \hline \(BCL2\) & 75,243 \(\pm\) & 56,350 \(\pm\) & 62,225 \(\pm\) & 23,101 \(\pm\) & 15,527 \(\pm\) & NS \\ (B-cell lymphoma 2) & 103,092 & 9429 & 34,664 & 5323 & 5319 & NS \\ \hline \(BCLXL\) & 197,181 \(\pm\) & 271,883 \(\pm\) & 257,775 \(\pm\) & 158,491 \(\pm\) & 112,387 \(\pm\) & NS \\ (B-cell lymphoma— extra large) & 65,858 & 47,827 & 67,301 & 42,901 & 4472 & NS \\ \hline \multicolumn{8}{c}{_EAS_} \\ (Fas cell surface death & 334,825 \(\pm\) & 1,068,306 \(\pm\) & 1,029,560 & 1,087,450 \(\pm\) & 439,381 \(\pm\) & 227,213 \(\pm\) & NS \\ receptor) & 306,367 & 1,029,560 & 636,856 & 298,303 & 238,264 & NS \\ \hline \multicolumn{8}{c}{_FASL_} \\ (Fas ligand) & 356 \(\pm\) 421 & 74 \(\pm\) 89 & 149 \(\pm\) 85 & 50 \(\pm\) 50 & 51 \(\pm\) 27 & NS \\ \hline \(CASP9\) & 28,148 \(\pm\) & 37,381 \(\pm\) & 31,013 \(\pm\) & 14,529 \(\pm\) & 16,955 \(\pm\) & 0.004 \\ (caspase 9) & 13,279 & 5907 & 8629 & 4178 & 2902 & 0.004 \\ \hline \(CASP2\) & 116,188 \(\pm\) & 186,388 \(\pm\) & 178,963 \(\pm\) & 140,131 \(\pm\) & 108,763 \(\pm\) & \\ (caspase 2) & 30,287 & 15,879 & 14,011 & 24,821 & 21,211 & \(<\)0.001 \\ \hline \(CASP3\) & 299,713 \(\pm\) & 480,000 \(\pm\) & 511,438 \(\pm\) & 283,938 \(\pm\) & 244,044 \(\pm\) & NS \\ (caspase 3) & 137,632 & 94,438 & 167,428 & 43,039 & 168,991 & NS \\ \hline \multicolumn{8}{c}{_TNF_} \\ (tumor necrosis factor) & 17,930 \(\pm\) & 37,638 \(\pm\) & 40,500 \(\pm\) & 6992 & 14,412 \(\pm\) & 23,328 \(\pm\) & \\ (tumor necrosis factor receptor 1) & 4651 & 4096 & 6992 & 6424 & 8232 & \(<\)0.001 \\ \hline \multicolumn{8}{c}{_TNF1_} \\ (tumor necrosis factor receptor 1) & 6,600,625 \(\pm\) & 8,998,125 \(\pm\) & 8,421,875 \(\pm\) & 6,648,750 \(\pm\) & 3,122,875 \(\pm\) & \\ (tumor necrosis factor receptor 1) & 4,298,333 & 2,632,911 & 1,566,133 & 2,222,046 & 1,240,915 & 0.049 \\ \hline \multicolumn{8}{c}{C—control cells; F—NaF-treated cells; F + SMF1—NaF-treated cells and exposed to SMF with 0.55 T flux density; F + SMF2—NaF-treated cells and exposed to SMF with 0.55 T flux density; F + SMF3—NaF-treated cells and exposed to SMF with 0.65 T flux density; the results are expressed as the means \(\pm\) SD; statistical significance: \({}^{*}p<0.05\), the one-way ANOVA test, NS—not statistically significant.} \\ \end{tabular}
\end{table}
Table 2: The expression of the genes that are associated with the apoptosis process in fluoride-treated fibroblasts and in fibroblasts that had been treated with fluoride, and then subjected to the influence of SMFs of different intensities (0.45, 0.55, and 0.65 T) for 12 h compared to the control culture without fluoride and without the magnet (flux density 0.0 T).