## 3 Results  
The identified metabolites of _E. planum_ callus include various organic acids, saccharides, phenolic acids, flavonoids, and triterpen saponins (Table 1). Among the notable compounds are maleic acid and methylmalonic acid, both classified as dicarboxylic acids and derivatives. The presence of citric acid, a widely known carboxylic acid, indicates a role in primary metabolism. Phenolic acids such as caffe acid, trans-caffeic acid, chlorogenic acid, _trans_-cumaric acid, neochlorogenic acid, _trans_-cumaric acid, ferulic acid, and rosmarinic acid were also detected in the callus extract. Additionally, vanillic acid, a methoxybenzoic acid derivative, contributes to the phenolic profile. Furthermore, several triterpen saponins, whose specific structures remain unidentified, are present, suggesting a role in plant defense and bioactivity. The flavonoid glycosides present in this species include kaempfer-3-\(O\)-rutinoside and 3-O-malonylglucose. Overall, the metabolite profile of _E. planum_ callus culture highlights a diverse array of bioactive compounds, many of which are associated with biological activities. Table 2 presents the main metabolites identified in the callus of _L. flos-cuculi_ (Table 2). The identified compounds include hydroxycinnamic acid derivatives--ferulic acid, caffe acid, \(p\)-cumaric acid, and other phenolic compounds, e.g., 1-O-caffeoylglucose, 1-O-feruloylglucose, and 4-hydroxybenzoic acid. Several triterpen saponins are detected, though their specific structures remain unidentified. The table also includes vitexin \(O\)-rhammoside, kaempfer 3-rutinoside, rutin, and apigenin, which are classified as flavonoids. Furthermore, some unknown compounds are categorized as carboxylic acids or phenolic/flavonoid derivatives, indicating the presence of yet-to-be-characterized metabolites. Overall, the table highlights a diverse metabolite profile in _L. flos-cuculi_ callus cultures, including organic acids, flavonoids, and saponins. Table 3 presents the main metabolites identified in the callus of _K. elatine_ (Table 3). The table includes phenylalanine and tryptophan. Pantothenate is listed under secondary alcohols, while 2-isopropylmalic acid falls under hydroxy fatty acids. Several phenyl-propanoids have been identified, including echinacoside, lavandulfiolioside, acetoside, isoatecoside, and leucoscopetoside. Additionally, caffeic acid and _trans_-4-cumaric acid are classified as hydroxycinnamic acids. The table also lists hydroxysebacic acid and azelaic acid. Long-chain fatty acids include (Z)-5,8,11-trihydroxyoctadec-9-enoic acid, while linoleic acids and their derivatives, such as FA 18:3+2O, 9-HODE, and FA 18:1+1O, are categorized under linoleic acids and derivatives. Overall, the metabolite profile of _K. elatine_ callus culture includes amino acids, phenylpropanoids, hydroxycinnamic acids, and fatty acids, highlighting its diverse composition. Tables 4-9 present the inhibitory/stimulatory effects of the reference compounds (Figure 2) and callus extracts of _E. planum_, _L. flos-cuculi_, and _K. elatine_ on _Acanthamoeba_ trophozoites over a three-day treatment period. The table includes mean values (MN \(\pm\) SD) and the percentage of growth inhibition or stimulation (% GS/GI) at different concentrations compared to the control.