# Plant In Vitro Cultures of _Coleus scutellarioides_ (L.[PERSON] [PERSON].
## 2 Results
### Volatile Compounds Composition
The most intense biosynthesis of volatile compounds took place in the plants cultivated on the medium enriched with NAA, whereas the least intense was observed on the medium supplemented with BA, where it was recorded at the level of 5610.02 ug g\({}^{-1}\). Irrespective of the plant growth regulator added to the breeding medium, supplementation was found to reduce the biosynthesis of 2-hexen-1-ol, \(\alpha\)-phellandrene, p-cymene, and nonanal. On the other hand, the biosynthesis of camphene, \(\beta\)-pinene, boranyl acetate, \(\beta\)-myrcene, and eucalypt was observed to increase, irrespective of the phytohormone applied to the breeding medium. BA caused the highest intensification in the biosynthesis of 3-hexen1-ol acetate, by 4.5 times. Of the employed plant growth regulators, it was only BA which caused a decline in the biosynthesis of linalool, \(\beta\)-elemene, \(\delta\)-guaiene, \(\alpha\)-selinene, and \(\gamma\)-selinene. Among the phytohormones used, fluoride most significantly enhanced the biosynthesis of \(\alpha\)-muurolene (1.2 times), \(\alpha\)-pinene (1.3 times), limonene (1.4 times), \(\beta\)-pinene (1.5 times), santalene (2.1 times), linalool and cyclosativene (1.6 times), \(\delta\)-guaiene (1.9 times), sabinen (3.0 times), and \(\beta\)-chamigrene (7.9 times). On the other hand, fluoride was responsible for the largest drop in the biosynthesis of 1-hexanol and di-_epi-_\(\alpha\)-cedrene. As the only one of the applied plant growth regulators, NAA supplemented in the breeding medium improved the biosynthesis of 1-octen-3-ol, 3-octanol, 3-hexen-1-ol, 2-hexanal, and 1-hexanol. This phytohormone was found to most significantly stimulate the biosynthesis of 2-hexen (1.1 times), 3-hexen-1-ol and \(\gamma\)- and \(\delta\)-cadinene (1.2 times), 1-octen-3-ol, 3-octanol and 1-hexanol (1.3 times), copaene (1.5 times), \(\alpha\)-selinene (1.7 times), \(\beta\)-elemene (2.0 times), and eucalypt (8.6 times). IAA was the only plant growth regulator supplemented in the breeding medium that was recorded to have a negative impact on the biosynthesis of \(\alpha\)-pinene. However, this phytohormone was found to be the best stimulating agent for the biosynthesis of 3-octanone (1.4 times), germacrene-D (1.8 times), caryophyllene (2.3 times), di-_epi-_\(\alpha\)-cedrene (3.3 times), camphene (9.9 times), and boranyl acetate (62 times). The addition of BA to the medium induces a clear decreasing trend in the context of the content of individual volatile compounds; however, in the case of \(\alpha\)-pinene, camphene, sabinene, \(\beta\)-pinene, boranyl acetate, \(\beta\)-myrcene, 3-hexen-1-ol, acetate, limonene, and eucalypt, it stimulates biosynthesis. Fluidone and IAA stimulated biosynthesis for 17 compounds, while it decreased biosynthesis for 16 compounds. No stimulatory or inhibitory trend is observed. In the case of NAA, a clear stimulating trend is observed for the biosynthesis of volatile compounds, with the exception of 2-hexen-1-ol, \(\alpha\)-phellandrene, p-cymene, and nonanal, for which showed a decreasing trend (Figure 4a-c). The calculated correlation coefficients generally confirmed, for most of the analyzed compounds, an increase in their biosynthesis with phytohormone supplementation (Supplementary Table S1). Figure 4: Contents of volatile compounds in _C. scutellarioides_ “Electric Lime” tissues: (**a–c**) contents of volatile compounds in plants cultivated in vitro with an addition of plant growth regulators as compared with those of the control.