## 3 Results and Discussion
### Gene Prediction of Holocellulose Degrading Enzymes in A. olivimuriae
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Culture** & **Cellulose** & **Hemicellulose** & **Klason Lignin** & **Reducing Sugars** & **Crude Lipids** & **Total Phenols** \\  & **(g kg\({}^{-1}\))** & **(g kg\({}^{-1}\))** & **(g kg\({}^{-1}\))** & **(g kg\({}^{-1}\))** & **(g kg\({}^{-1}\))** & **(g kg\({}^{-1}\))** \\ \hline RTB (IC) & 405.6 \(\pm\) 12.1 c & 187.0 \(\pm\) 3.3 c & 201.1 \(\pm\) 8.0 a & 3.3 \(\pm\) 0.6 a & 20.9 \(\pm\) 2.0 b & 17.3 \(\pm\) 2.3 a \\ RTB (SSC) & 354.2 \(\pm\) 4.0 b & 121.9 \(\pm\) 4.4 a & 217.5 \(\pm\) 2.2 a & 17.7 \(\pm\) 2.2 b & 14.5 \(\pm\) 1.3 a & 38.3 \(\pm\) 4.2 c \\ RTB + WB (IC) & 365.6 \(\pm\) 13.4 b & 167.7 \(\pm\) 6.2 b & 181.2 \(\pm\) 14.4 a & 3.7 \(\pm\) 0.5 a & 26.5 \(\pm\) 2.1 c & 15.6 \(\pm\) 3.4 a \\ RTB + WB (SSC) & 279.6 \(\pm\) 16.3 a & 123.4 \(\pm\) 12.1 a & 204.8 \(\pm\) 19.5 a & 16.4 \(\pm\) 0.6 b & 15.3 \(\pm\) 3.2 a & 23.8 \(\pm\) 2.8 b \\ \hline SCG (IC) & 119.2 \(\pm\) 3.0 b & 370.2 \(\pm\) 16.3 c & 199.4 \(\pm\) 18.5 b & 3.2 \(\pm\) 0.0 a & 139.0 \(\pm\) 6.4 c & 48.7 \(\pm\) 3.2 b \\ SCG (SSC) & 105.4 \(\pm\) 4.4 a & 326.3 \(\pm\) 18.3 b & 240.0 \(\pm\) 21.3 c & 7.6 \(\pm\) 1.1 b & 109.2 \(\pm\) 4.1 b & 56.0 \(\pm\) 2.3 c \\ SCG + WB (IC) & 136.2 \(\pm\) 32.2 c & 337.1 \(\pm\) 8.6 b & 171.4 \(\pm\) 52.2 a & 3.1 \(\pm\) 0.5 a & 121.2 \(\pm\) 8.2 b & 40.8 \(\pm\) 2.1 a \\ SCG + CG (SSC) & 103.5 \(\pm\) 3.1 a & 255.4 \(\pm\) 21.6 a & 176.5 \(\pm\) 5.2 ab & 9.6 \(\pm\) 0.4 c & 75.8 \(\pm\) 10.3 a & 51.5 \(\pm\) 2.6 b \\ \hline BS (IC) & 420.1 \(\pm\) 3.0 d & 279.5 \(\pm\) 11.2 c & 165.0 \(\pm\) 14.3 a & 41.3 \(\pm\) 2.8 a & 15.6 \(\pm\) 1.1 a & 20.7 \(\pm\) 2.1 ab \\ BS (SSC) & 393.9 \(\pm\) 3.2 c & 237.0 \(\pm\) 9.3 b & 172.6 \(\pm\) 8.2 a & 51.3 \(\pm\) 2.0 b & 12.0 \(\pm\) 1.9 a & 27.1 \(\pm\) 4.3 b \\ BS + WB (IC) & 376.1 \(\pm\) 8.3 b & 262.7 \(\pm\) 8.3 c & 159.0 \(\pm\) 14.8 a & 43.4 \(\pm\) 4.6 a & 21.4 \(\pm\) 2.4 b & 18.4 \(\pm\) 4.2 a \\ BS + WB (SSC) & 339.7 \(\pm\) 5.0 a & 210.4 \(\pm\) 7.6 a & 170.6 \(\pm\) 13.3 a & 40.8 \(\pm\) 5.4 a & 20.3 \(\pm\) 3.0 b & 25.4 \(\pm\) 3.0 b \\ \hline DSF (IC) & 393.2 \(\pm\) 4.6 c & 174.1 \(\pm\) 12.3 c & 257.2 \(\pm\) 11.6 b & 10.9 \(\pm\) 0.5 b & 19.2 \(\pm\) 3.1 b & 15.6 \(\pm\) 1.6 b \\ DSF (SSC) & 363.0 \(\pm\) 7.1 b & 70.2 \(\pm\) 9.1 a & 272.0 \(\pm\) 8.3 c & 4.6 \(\pm\) 0.3 a & 21.4 \(\pm\) 0.2 b & 11.9 \(\pm\) 2.0 a \\ DSF + WB (IC) & 354.2 \(\pm\) 4.1 b & 177.3 \(\pm\) 8.6 c & 220.3 \(\pm\) 4.8 a & 17.4 \(\pm\) 3.1 c & 22.4 \(\pm\) 3.1 b & 14.3 \(\pm\) 1.3 a \\ DSF + WB (SSC) & 321.3 \(\pm\) 12.0 a & 92.7 \(\pm\) 8.3 b & 236.1 \(\pm\) 5.2 b & 4.9 \(\pm\) 0.1 a & 4.4 \(\pm\) 0.2 a & 12.9 \(\pm\) 1.1 a \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Contents of cellulose, hemicellulose, Klason lignin, reducing sugars, total phenols and crude lipids in non-inoculated incubation controls (IC) and 14-day-old _A. olivimuriae_ solid-state cultures (SSC) on residual thistle biomass (RTB), spent coffee grounds (SCG), barley straw (BS) and digestate solid fraction (DSF) in the absence and in the presence of wheat bran (WB). Data represent the mean \(\pm\) standard deviation of 4 replicates. Multiple pairwise comparisons were carried out within each matrix using the Tukey test, and identical letters denote that column means within each block were not significantly different (\(p>0.05\)). by [PERSON] et al. [58], and compared to _Aspergillus_ species already characterized for their CAZyme content (Table 4) [3]. A significant number of enzymes related to cellulose, hemicellulose and pectin degradation was identified [2; 3].