## 2 Results and Discussion
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multirow{2}{*}{**Name**} & \multirow{2}{*}{**RI \({}^{\star}\)**} & \multirow{2}{*}{**RT \({}^{\star}\)**} & \multicolumn{4}{c}{**Mating Stages**} & \multirow{2}{*}{_p Value_} & \multirow{2}{*}{**FDR \({}^{\ddagger}\)**} \\ \cline{5-6}  & & & & **Before** & & **During** & **After** \\ \hline N-methyleneethanamine & 749.4 & 1.312 & 162.767 & N.D \({}^{\circ}\) & N.D \({}^{\circ}\) & 0.005 & 0.023 \\ Ethyl glycolate & 780.5 & 1.954 & N.D \({}^{\circ}\) & N.D \({}^{\circ}\) & 259.978 & 0.011 & 0.031 \\
2,5-Dihydroxbenzaldehyde & 1123.1 & 8.720 & 100.924 & N.D \({}^{\circ}\) & N.D \({}^{\circ}\) & 6.250 & 0.003 \\ Acetic acid 2-propyltetrahydropropyl-3-yl ester & 1181.3 & 9.551 & N.D \({}^{\circ}\) & 283.245 & N.D \({}^{\circ}\) & 0.010 & 0.031 \\ Dididorp-methyl & 1266.7 & 11.602 & N.D \({}^{\circ}\) & 78.171 & N.D \({}^{\circ}\) & 0.008 & 0.027 \\
1,2-Dihydro-2,2,4-trimethylquinoline & 1452.6 & 15.297 & 34.924 & 119.575 & N.D \({}^{\circ}\) & 0.018 & 0.041 \\ e-Farnesne & 1513.7 & 16.367 & 281.554 & 190.567 & N.D \({}^{\circ}\) & 0.001 & 0.009 \\ Decanoic acid octyl ester & 1650.5 & 18.601 & 116.138 & N.D \({}^{\circ}\) & N.D \({}^{\circ}\) & 0.021 & 0.043 \\ Dodecane, I-iodo- & 1716.2 & 19.656 & N.D \({}^{\circ}\) & N.D \({}^{\circ}\) & 108.690 & 0.003 & 0.019 \\ Tetradecanoid acid & 1765.4 & 0.432 & 700.0986 & N.D \({}^{\circ}\) & 88.350 & 0.005 & 0.024 \\
2,6,10,15-Tetramethylheptadecane & 1892.7 & 22.466 & 52.3699 & 1066.241 & 176.519 & 0.008 & 0.027 \\ Hexadecanoic acid methyl ester & 1917.5 & 2.861 & 759.908 & 1283.292 & N.D \({}^{\circ}\) & 0.022 & 0.043 \\ Hexadecanoic acid pyrrolide & 1937.7 & 23.182 & N.D \({}^{\circ}\) & 1168.109 & N.D \({}^{\circ}\) & 0.000 & 0.008 \\
9-Hexadecenoic acid pyrrolide & 1944.1 & 23.182 & 382.040 & N.D \({}^{\circ}\) & 757.991 & 0.001 & 0.027 \\
1-Piperidin-1-yl-hexadecan-1-one & 1958.6 & 23.518 & 982.573 & N.D \({}^{\circ}\) & 1095.741 & 0.008 & 0.027 \\
9,12-(_Z_,_Z_)-Octadecanenoic acid & 2078.2 & 25.428 & 356.887 & 2684.126 & N.D \({}^{\circ}\) & 0.021 & 0.043 \\ Methyl stearate & 2105.0 & 25.5849 & 229.924 & 294.663 & N.D \({}^{\circ}\) & 0.006 & 0.026 \\ Henecioyal acetate & 2181.3 & 27.073 & 56.3354 & N.D \({}^{\circ}\) & N.D \({}^{\circ}\) & 0.001 & 0.009 \\
9,(_Z_)-tricosene & 2241.4 & 28.066 & N.D \({}^{\circ}\) & N.D \({}^{\circ}\) & 209.611 & 0.001 & 0.012 \\ Trimneyfluorane & 2672.6 & 34.896 & 31.653 & N.D \({}^{\circ}\) & 1398.338 & 0.025 & 0.049 \\
1,4-Benzenedicarboxylic acid bis-2-ethylhexyl ester & 2679.7 & 35.001 & N.D \({}^{\circ}\) & 1345.4 & N.D \({}^{\circ}\) & 0.017 & 0.041 \\
9,11-Didehydrodrumiliated acetate & 2865.1 & 37.957 & 652.982 & N.D \({}^{\circ}\) & 495.747 & 0.001 & 0.012 \\ Stigmasta-3,5-diene & 2967.1 & 39.578 & N.D \({}^{\circ}\) & 225.929 & 0.009 & 0.009 & 0.029 \\
9-Sitosterol acetate & 2968.5 & 39.601 & 86.1762 & N.D \({}^{\circ}\) & 403.452 & 0.013 & 0.034 \\ Octatriacontyl pentafluoropropionate & 2991.1 & 39.964 & N.D \({}^{\circ}\) & N.D \({}^{\circ}\) & 70.866 & 0.003 & 0.018 \\
2,6-Tobeny1 acetate & 2995.1 & 40.029 & 152.892 & N.D \({}^{\circ}\) & N.D \({}^{\circ}\) & 0.011 & 0.031 \\
38,22(_E_)-Ergosta-5,8,22-rien-3-ol & 3055.5 & 40.981 & N.D \({}^{\circ}\) & 217.940 & N.D \({}^{\circ}\) & 0.007 & 0.009 \\
3-Stigmasta-5,22-diene-3-ol acetate & 3094.1 & 41.611 & 259.867 & 231.980 & N.D \({}^{\circ}\) & 0.004 & 0.023 \\
9-Hexadecenoic acid hexadecary ester & 31313.3 & 42.196 & N.D \({}^{\circ}\) & N.D \({}^{\circ}\) & 509.690 & 0.014 & 0.036 \\
11,13-Dimethyl-12-tetadecan-1-ol acetate & 3137.0 & 42.888 & N.D \({}^{\circ}\) & N.D \({}^{\circ}\) & 176.459 & 0.003 & 0.018 \\
3189.3 & 43.124 & N.D \({}^{\circ}\) & N.D \({}^{\circ}\) & 139.731 & 0.019 & 0.041 \\
9-(_E_)-Octadecenoic acid, & 3251.9 & 44.119 & N.D \({}^{\circ}\) & N.D \({}^{\circ}\) & 600.066 & 0.001 & 0.012 \\
9-Hexadecenoic acid octadecenyl ester & 3257.9 & 44.219 & 303.882 & N.D \({}^{\circ}\) & 158 Figure 1: Score plots from Sparse Partial Least Squares-Discriminant Analysis (sPLS-DA) analyzed based on the total peak area obtained from GC-MS data of DI-SPME samples from three different mating stages of medfly: +, before mating; \(\times\), during mating; \(\Delta\), after mating using two solvents (**A**) and (**B**).