## 3 Results
### Degradation of Individual Pharmaceutical Compounds by the Ascomyce P. oxalicum at Flask-Scale
(**a**) Control system = Active biomass without glucose supplementation (**b**) Feed system = Active biomass with 3 g/L of glucose supplementation every 48 h. The biotic control did not show a significant difference and was similar to individual biodegradation (data not shown). Key: acetaminophen (APAP); diclofenac (DFC); ibuprofen (IBU); ketoprofen (KET); and naproxen (NPX). The values are presented as the average of three independent experiments \(\pm\) standard deviation (\(n\) = 3). Asterisks (*) indicates statistical significance differences using Tukey test (\(p\) < 0.05). throughout the entire process. Temperature and dissolved oxygen did not have significant changes. At this point it is important to note that the fact that the concentration of dissolved oxygen in the medium was low indicates that the oxygen is being used by the fungus in an aerobic process, which prevents accumulation of oxygen in the culture medium. By-Products Obtained during the Biotransformation of the NSAIDs/Analgesic Mixture by Penicillin oxalicum  
Characteristics of the different by-products found in the degradation of the tested pharmaceutical compounds are represented in Table 2. The hydroxylated metabolites predominated in the \begin{table}
\begin{tabular}{c c c c c c c c c c} \hline \hline \multirow{2}{*}{**Time (h)**} & \multicolumn{2}{c}{**DFC**} & \multicolumn{2}{c}{**IBU**} & \multicolumn{2}{c}{**KET**} & \multicolumn{2}{c}{**NPX**} & \multicolumn{2}{c}{**APAP**} \\ \cline{2-10}  & **g+** & **g-** & **g+** & **g-** & **g+** & **g-** & **g+** & **g-** & **g+** & **g-** & **g+** & **g-** \\ \hline
0 & a & a & a & a & a & a & a & a & a & a \\
24 & a & b & a & a & a & b & a & b & a & a \\
48 & a & b & a & a & a & a & a & b & a & a \\
72 & a & b & a & a & a & b & a & b & a & a \\
96 & a & b & a & a & a & b & a & b & a & a \\
120 & a & b & a & a & a & b & a & b & a & a \\
**Global Behavior** & Affected & Not affected & & & & & & & & \\  & & & & & & & & & & \\ \hline \hline g+ = 3 g/L glucose supplementation; g- = Without glucose supplementation. APAP = acetaminophen; DFC = diclofenac; IBU = ibuprofen; KET = ketoprofen; NPX = naproxen. Different letters indicate statistical significance difference using Tukey test (\(p<0.05\)). & & & & & & \\ \hline \hline \end{tabular}  
* g+ = 3 g/L glucose supplementation; g- = Without glucose supplementation. APAP = acetaminophen; DFC = diclofenac; IBU = ibuprofen; KET = ketoprofen; NPX = naproxen. Different letters indicate statistical significance difference using Tukey test (\(p<0.05\)).  
\end{table} 
Table 1: Statistical analysis of the influence of glucose supplementation in the biodegradation of NSAIDs/analgesic complex mixture by _P. oxalicum_. Figure 3: Biodegradation evolution of the NSAIDs/analgesic mix at batch feed bioreactor scale by _Penicillin oxalicum_. A liquid growth degradation system incubated for 192 h was used under agitation and aeration. Key: acetaminophen (APAP); diclofenac (DFC); ibuprofen (IBU); ketoprofen (KET); and naproxen (NPX). degradation of the complex mixture, seeing as they were present in the degradation of all the tested pharmaceutical compounds. In Figure 4, the partial degradation pathway of each drug is represented. In this case, the elimination of the metabolites of the NSAIDs biodegradation is not possible since existed metabolite accumulation, especially KET by-products.