## 4 Conclusions  
In this study, an HPLC analysis method using major marker components was developed for quality control of DSHT, an herbal medicine prescription used in Soyangangmyunghabbyung. The developed method was validated with respect to linearity, LOD, LOQ, recovery, and precision, and has been successfully applied to the simultaneous analysis of DSHT. \begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline  & \multicolumn{6}{c}{**Amount (mg/g Freeze-Dried Sample)**} \\ \cline{2-9}
**Analyte** & \multicolumn{4}{c}{**Sample 1 1 **} & \multicolumn{4}{c}{**Sample 2 **} & \multicolumn{4}{c}{**Origin 3 **} \\ \cline{2-9}  & \multicolumn{2}{c}{**Batch 1 **} & \multicolumn{2}{c}{**Batch 2 **} & \multicolumn{2}{c}{**Batch 1 **} & \multicolumn{2}{c}{**Batch 2 **} \\ \cline{2-9}  & **Mean** & **RSD (\%)** & **Mean** & **RSD (\%)** & **Mean** & **RSD (\%)** & **Mean** & **RSD (\%)** & \\ \hline Gallic acid & 2.62 & 0.46 & 2.62 & 0.52 & 0.84 & 0.74 & 0.84 & 0.25 & PL \\ Albiforin & 1.52 & 1.02 & 1.48 & 1.50 & 2.41 & 0.05 & 2.39 & 0.24 & PL \\ Paeconiflorin & 7.72 & 0.31 & 7.75 & 0.43 & 4.94 & 0.04 & 4.93 & 0.16 & PL \\ Naringin & 5.05 & 0.35 & 5.03 & 0.35 & 1.10 & 0.26 & 1.09 & 0.54 & PT \\ Benzoic acid & 0.96 & 0.37 & 0.96 & 1.17 & 0.29 & 0.29 & 0.29 & 0.17 & PL \\ Bacialin & 46.17 & 0.44 & 45.79 & 0.33 & 39.34 & 0.52 & 36.86 & 0.02 & SB \\ Poncirin & 7.57 & 0.07 & 7.53 & 0.28 & ND 4  & - & ND & – & PT \\ Wogonoside & 8.32 & 0.21 & 8.35 & 0.26 & 1.09 & 0.03 & 1.10 & 0.16 & SB \\ Baiclein & 3.77 & 0.47 & 3.78 & 0.49 & 1.78 & 0.16 & 1.77 & 0.05 & SB \\ Wogonin & 0.77 & 0.16 & 0.77 & 0.17 & 0.51 & 0.10 & 0.51 & 0.14 & SB \\ \hline \hline \end{tabular}  
* * DSHT sample manufactured by KIOM. 4
* DSHT sample of commercial products manufactured by a Korean pharmaceutical company. 5
* _lactiflora_; PT–_P. trifoliata_; SB–_S. baicalensis_. 5
* Not detected.  
\end{table} 
Table 4: Concentrations of the 10 markers in the DSHT samples determined with the HPLC–DAD assay (\(n=3\)). \begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline  & \multicolumn{6}{c}{**Intraday**} & \multicolumn{3}{c}{**Interday**} \\ \cline{2-9}
**Analyte** & **Con. (ug/mL)** & **Measured Conc. (ug/mL)** & **Precision** & **Accuracy (\%) 1 **} & **Accuracy (\%) 2 ** & **Measured Conc. (ug/mL)** & **Precision Conc. (ug/mL)** & **Precision** (RSD, \%)** & **Accuracy (\%)** \\ \hline Baicalin & 12.50 & 12.47 & 1.03 & 99.74 & 12.62 & 1.54 & 100.94 \\  & 25.00 & 24.46 & 0.25 & 97.83 & 24.65 & 1.26 & 98.59 \\  & 50.00 & 48.64 & 0.33 & 97.28 & 48.78 & 0.99 & 97.55 \\ \hline Poncirin & 25.00 & 26.43 & 0.70 & 105.72 & 26.55 & 0.92 & 106.19 \\  & 50.00 & 49.80 & 0.45 & 99.59 & 49.68 & 0.58 & 99.35 \\  & 100.00 & 96.86 & 0.33 & 96.86 & 97.11 & 0.64 & 97.11 \\ \hline Wogonoside & 25.00 & 26.44 & 0.76 & 105.74 & 26.68 & 1.32 & 106.72 \\  & 50.00 & 52.89 & 0.46 & 105.78 & 52.92 & 1.04 & 105.85 \\  & 100.00 & 105.29 & 0.43 & 105.29 & 104.84 & 0.85 & 104.84 \\ \hline Baicalein & 12.50 & 13.16 & 0.65 & 105.28 & 12.99 & 1.13 & 103.94 \\  & 25.00 & 25.26 & 0.35 & 101.05 & 25.26 & 1.12 & 101.04 \\  & 50.00 & 50.57 & 0.65 & 101.14 & 50.54 & 1.17 & 101.08 \\ \hline Wogonin & _7.50_ & _7.57_ & 0.41 & 100.87 & _7.60_ & 0.78 & 101.29 \\  & 15.00 & 15.34 & 0.50 & 102.25 & 15.40 & 0.65 & 97.92 \\  & 30.00 & 30.22 & 0.63 & 100.74 & 30.21 & 0.39 & 100.69 \\ \hline \hline \end{tabular}  
* * A Accuracy = measured concentration/concentration \(\times\) 100.  
\end{table} 
Table 3: _Cont._The method developed in this study will provide useful basic data for establishing analysis methods for the quality control of other TCMs, TKMs, and KMs in addition to DSHT. The following are available online at [[https://www.mdpi.com/article/10.3390/app112110242/s1](https://www.mdpi.com/article/10.3390/app112110242/s1)]([https://www.mdpi.com/article/10.3390/app112110242/s1](https://www.mdpi.com/article/10.3390/app112110242/s1)), Figure S1: Chemical structures of the 10 marker components in DSHT; Figure S2: HPLC chromatogram of the mixed 16 standard solution (A) and the DSHT sample (B).