## 3 Results
### Effects of Streptomyces sp. Y1-14 Extracts on Foc TR4 Metabolites  
To further analyze the secondary metabolites of _Streptomyces_ sp. Y1-14 extracts-treated Foc TR4, the analysis of widely targeted metabolome was performed using the LC-ESI-MS/MS system. A total of 1003 metabolites were obtained in control and treatment samples (Figure 5A). The principal component analysis (PCA) revealed that PC1, PC2, and PC3 were 68.73%, 25.74%, and 3.98% in 3D diagram, respectively (Supplementary Figure S2A). Compared with the metabolites of Foc TR4 in the control group, the numbers of upregulated and downregulated metabolites after 6 h of treatment were 53 and 43, respectively (Supplementary Figure S2B). N10-Formyl-THF and virginze were significantly upregulated. 3-Butynoic acid, 2-dehydroromatoateand, and pteroyl-alpha-glutamyl glutamate were significantly downregulated (Figure 5B). According to functional annotation, N10-Formyl-THF and 2-dehydroromatoate belonged to the carbon metabolism pathway (ko01200) as well as pantotheane and CoA biosynthesis (ko01110), respectively. In the growth tested after treatment for 12 h, 122 metabolites showed differences, 104 metabolites were upregulated, and 18 metabolites were downregulated (Supplementary Figure S2C). Especially, cytochalasin B and geldanamycin were significantly upregulated. N5-Pan and geranylgeraniol were obviously downregulated (Figure 5C). A Total of 207 differentially expressed metabolites (DEMs) were majorly classified into organooxygen compounds, glycerophospholipids, carboxylic acids and derivatives, fatty acids, preenol lipids, and imidazopyrimidines, etc (Supplementary Figure S2D,E). The Venn diagram was used to further analyze the intersection and uniqueness of metabolites in different differential groups. Eleven differential \begin{table}
\begin{tabular}{c c c c} \hline \hline \multicolumn{1}{c}{**RT a (min)**} & **Possible Compound b,c ** & **Relative Peak Area (\%)** & **Classification** \\ \hline
1.343 & Ala-Gly & 1.4163 & \\
2.081 & Ethyl Acetate & 1.0644 & \\
4.089 & Silanediol, dimethyl- & 1.0444 & Others \\
11.026 & 3-Furancarboxylic acid, methyl ester & 1.1225 & \\
30.66 & trans-1,10-Dimethyl-trans-9-decalinol & 21.05 & \\ \hline
12.949 & \(p\)-Menth-8-ene, 3-methylene- & 3.9783 f & \\
22.687 & Naphthalene, 1,2,3,4,4a,5,6,8a-octahydro-4a,8-dimethyl- & 9.5415 & \\
22.894 & Cyclohexane, 1,1,4,4-tetramethyl-2,5-dimethylene- & 4.0992 & Hydrocarbons \\
23.551 & Bicyclo [4.3.0] non-3-ene, 3,4,7-trimethyl- & 1.1359 & Hydrocarbons \\
23.74 & (2Z)-2-ethylidene-1,7,7-trimethyl-bicyclo [2.2.1] heptane & 2.2966 & \\
30.34 & Tetradecane & 1.1357 & \\ \hline
20.836 & 2-Methylisoborneol & 11.9192 & \\
32.681 & Octahydro-2,2,5a,9-tetramethyl-2H-3,9a-methano-1- & 3.8177 & Alcohols \\
35.365 & Palustrol & 3.2699 & \\
39.452 & Nerolidol & 1.4601 & \\ \hline
28.035 & \(\beta\)-Chamigrene & 1.3231 & \\
29.732 & 1-ethenyl-1-methyl-2,4-bis(1-methyletheinyl)-, & 1.0102 & \\
[15-(1a,2a,4a)]- & & \\
30.759 & (\(-\))-\(\alpha\)-Cedrene & 2.179 & Terpene \\
32.357 & Guaia-10(14),11-diene & 2.7849 & \\
33.415 & \(\beta\)-Cubebee & 4.2486 & \\
34.095 & \(\gamma\)-Gurjurene & 1.6816 & \\
34.685 & \(\gamma\)-Cadinene & 1.0292 & \\
34.941 & Cadina-1(10),4-diene & 2.3372 & \\ \hline \end{tabular}
\end{table} 
Table 1: VOCs produced by _Streptomyces_ sp. Y1-14 by GC-MS analysis. metabolites were shared by two differential groups (Figure 5D). The results of KEGG annotation showed that eleven metabolites were clustered into pentose phosphate pathways and carbon metabolism pathways, suggesting that _Streptomyces_ sp. Y1-14 extracts affected the energy metabolism of Foc TR4. Figure 5: Effect of _Streptomyces_ sp. Y1-14 on secondary metabolism of Foc TR4. (**A**) Heatmap based on the metabolome data of Foc TR4 treated with extracts of _Streptomyces_ sp. Y1-14 at 0 h, 6 h, and 12 h. Three biological replications were carried out for analyzing the metabolite profiling. (**B**,**C**) Volcanic map of differential metabolites.