## 3 Results and Discussion
### Oxidative Stability of BCSO
#### 3.1.1 Specific Absorbance Coefficients  
The presence of conjugated diene (CD) and triene (CT) systems resulting from oxidation processes were determined by measuring the specific absororptivity at 232 and 268 nm. The calculated values from Equation (1) of \(K_{232}\) and \(K_{268}\), presented in Table 1, indicated the existence of primary and secondary oxidation products, respectively. The behavior of the obtained value of this parameters during storage at 60 \({}^{\circ}\)C was similar to that reported by [PERSON] [PERSON] (2016). No significance change in the \(K_{232}\) value was noticed up to 18 days of storage for the uncovered samples. Later, a slight acceleration was observed. Comparable results of \(K_{232}\) for BCSO were obtained by [PERSON] (2017), although, in this study, after 10 days of heating, a significant increase in the \(K_{232}\) value was noted from the initial 8 up to 27. In the case of the covered probes, \(K_{268}\) showed a smaller (no statistically differences) alteration at the end of the storage time. The observed differences between the open and closed samples' behavior were the result of the oxygen presence. It was indicated that oxygen accelerates the oxidation process in oils [PERSON] and [PERSON] (2016), so, in open probes, the primary oxidation products were created more efficiently. For the second absorption indicator, \(K_{268}\), no evidenced increase in these values during storage was observed for both kinds of oil probes. This is related to the fact that the amount of CT triene systems, as a result of the next step of oxidation, usually increased in the middle or late phase of this process Ramadan and Morsel (2018). Only a slight change in specific absorptivity values indicated that the BCSO was resistance to thermal treatment.  
\begin{table}
\begin{tabular}{c c c c} \hline \hline \multirow{2}{*}{**Day of Storage**} & \multicolumn{2}{c}{**Open Samples**} & \multicolumn{2}{c}{**Closed Samples**} \\ \cline{2-4}  & \(K_{232}\) & \(K_{268}\) & \(K_{232}\) & \(K_{268}\) \\ \hline
0 & 6.02 \(\pm\) 0.58 a & 1.34 \(\pm\) 0.18 ab & 6.02 \(\pm\) 0.58 a & 1.34 \(\pm\) 0.18 a \\
3 & 5.99 \(\pm\) 0.33 a & 1.43 \(\pm\) 0.11 a & 6.56 \(\pm\) 0.51 a & 1.38 \(\pm\) 0.53 a \\
6 & 5.76 \(\pm\) 0.11 a & 1.31 \(\pm\) 0.05 ab & 6.02 \(\pm\) 0.43 a & 1.28 \(\pm\) 0.47 a \\
10 & 5.83 \(\pm\) 0.59 a & 1.11 \(\pm\) 0.15 b & 6.33 \(\pm\) 0.42 a & 1.61 \(\pm\) 0.17 a \\
14 & 5.91 \(\pm\) 0.09 a & 1.12 \(\pm\) 0.03 b & 6.78 \(\pm\) 0.32 ab & 1.59 \(\pm\) 0.17 a \\
18 & 7.17 \(\pm\) 1.05 ab & 1.02 \(\pm\) 0.19 d & 6.78 \(\pm\) 0.39 ab & 1.66 \(\pm\) 0.15 a \\
21 & 8.54 \(\pm\) 0.72 b & 1.04 \(\pm\) 0.08 cd & 7.01 \(\pm\) 1.01 ab & 1.39 \(\pm\) 0.25 a \\
24 & 8.57 \(\pm\) 0.62 b & 1.09 \(\pm\) 0.04 d & 7.46 \(\pm\) 0.52 b & 1.25 \(\pm\) 0.30 a \\ \hline \hline \end{tabular} The data in the table are presented as the means \(\pm\) standard deviation (SD). a–d Means within columns with different superscripts are significantly different (\(p<0.05\)).  
\end{table}
Table 1: The specific absorbance coefficient \(K_{232}\) and \(K_{268}\) for covered (“closed”) and uncovered (“open”) BCSO samples during storage at 60 \({}^{\circ}\)C.