## 3 Results and Discussion
### Analysis of Plasmid Presence and Antibiotic Resistance Genes
#### 3.6.5 NRPS and PKS Gene Clusters
Proteins XJI16076 and XJI16057 are homologous to DpgB and DpgD, respectively, and are known to enhance the efficiency of the reactions catalyzed by DpgA. Protein XJI16058 is homologous to DpgC, a cofactor-independent dioxygenase that contributes to the conversion of (3,5-dihydroxyboxphenyl)acetyl-CoA to (3,5-dihydroxyboxphenyl)oxoacetic acid. The (3,5-dihydroxyboxphenyl)glyoxylic acid produced by the DpgA-D proteins is a biosynthetic product with a conserved structure in actinobacteria that produce biparylpolyketides, which can be converted into the non-proteinogenic amino acid (3,5-dihydroxyboxphenyl)glycine, commonly found in antibiotics such as kendomycin and venemycin [67; 68; 69]. Additionally, it can be inferred that protein XJI16079 catalyzes the thiamine diphosphate (TDP)-dependent decarboxylation of (3,5-dihydroxyboxphenyl)oxoacetic acid to yield 3,5-dihydroxyboxpalaldehyde. However, genes corresponding to aldehyde dehydrogenases that oxidize 3,5-dihydroxyboxpalaldehyde to 3,5-dihydroxyboxrazole acid, such as VemF and Ken6, were not identified in the region 10 gene cluster. Macrolides synthesized by Type I modular PKS consist of macrocyclic lactones of varying ring sizes, to which one or more deoxy sugar or amino sugar residues are attached [71]. In this context, we further identified the presence of macrolide glycosyltransferase (protein XJI16074) and three macrolide sugar-O-methyltransferaseases (proteins XJI16072, XJI16073, and XJI16075) within BGC region 10. Ultimately, we conclude that the genes in BGC region 10 may play a role as starter units in the assembly of 18-membered polyketides with a single sugar moiety attached, synthesized by the Type I modular PKS. BGC Region 11 Derived from _Lentzea_ sp. JNUCC 0626  
BGC region 11 contains a single modular PKS module with the 1907 proteins composed of KS-AT-DH-KR-TE domains. Analysis using data from repositories such as the MIBiG database through KnownClusterBlast revealed no significant similarity to previously characterized BGCs (Figure S16, Table S26). BGC Region 12 Derived from _Lentzea_ sp. JNUCC 0626  
The BGC region 12 of _Lentzea_ sp. JNUCC 0626 contains several key components, as depicted in Figure S17 and outlined in Table S27. This region includes three NRPS-related proteins (proteins XJI16425, XJI16428, and XJI16433). In addition, it encompasses more than five regulatory genes of various types, one transporter protein, and an MbtH family protein (protein XJI16429). Notably, region 12 is characterized by proteins associated with typical vancomycin resistance mechanisms, including vancomycin resistance histidine kinase VanS (protein XIJ16438), D-Ala-D-Ala dipeptidase VanX (protein XIJ16439), D-alanine-(R)-lactate ligase VanA-Sc (protein XIJ16440), and vancomycin resistance protein VanH (protein XIJ16441). The BGC region 15 Derived from _Lentzea_ sp. JNUCC 0626 consists of two key nonribosomal peptide synthetases (NRPSs), as illustrated in Figure S18 and detailed in Table S28. This region includes an NRPS comprising four modules (Ile-D-Val-D-Thr-Val) represented by protein XIJ17060, and another NRPS containing five modules (D-Vak-Orn-D-Orn-Orn-D-X) represented by protein XIJ17059. Additionally, this region harbors more than two regulatory genes of various types, one transporter protein, one \(\beta\)-lactamase, and an MbtH family protein (protein XIJ17062). BGC Region 16 Derived from _Lentzea_ sp. JNUCC 0626  
_Lentzea_ sp. JNUCC 0626 BCG region 16 comprises a large assembly of 10 modular PKS proteins, spanning proteins XIJ17218 to XIJ17225 and XIJ19137 (Figure S19 and Table S29). Specifically, protein XIJ17225 contains three modules (KS-AT/KS-AT-DH-KR/KS-AT-DH-KR), while protein XIJ17224 has one module (KS-AT). Protein XIJ17223 includes two modules (KS-AT-/KS-AT/KR), protein XIJ17222 contains three modules (KS-AT-DH-KR/KS-AT-KR/KS-AT-DH-KR), and protein XIJ17221 consists of one module (KS-AT-DH-KR).