# Plant In Vitro Cultures of _Coleus scutellarioides_ (L.[PERSON] [PERSON].
## 3 Discussion  
### Discussion of In Vitro Cultivation  
The effective induction of aseptic tissue cultures was attained by using stems with nodal segments. Other scientific teams induced tissue culture in _C. scutellarioides_ from stems, leaves, or nodal segments comprising lateral buds and from apical shoots [27; 28; 29; 30].  
In our study, induction was successful after six weeks. Auxins are reported to positively affect the diversification of cells, elongational growth, and plant rooting. The auxins used in our research, which are NAA (naphthyl-1-acetate acid) and IAA (indolil-3-acetate acid), exerted such a stimulating influence on the studied plants. Although the differences were not statistically significant, in the case of _C. scutellarioides_ \"Electric Line\", the greatest number of shoots were observed when NAA had been added, but not in the case of supplementation with cytokins, which are known to abolish the apical dominance, thus causing plant branching. It is probable that there was a high amount of cytokins in the initial material, and therefore, no significant effect after their supplementation was recorded.  
In the case of plant in vitro culture, the application of appropriate physical factors, as well as hormones or their mixtures at a proper proportion, can induce a desirable morphological response of the plant. Investigations demonstrate that a combination of 0.5 mg dm\({}^{-3}\) GA3 (gibberellic acid-3) and 0.5 mg dm\({}^{-3}\) BAP (6-benzylaminopurine) caused a 1.4- and 1.3-fold increase, respectively, in the number of shoots and the plant height in comparison with those of the control. The application of 1.0 mg dm\({}^{-3}\) IBA (indolylbutyric acid) prompted the earliest appearance of the roots, accompanied by a nearly 2.3-fold increase in the number of roots per stem as compared with the control medium [30]. Research also shows that breeding _C. scutellarioides_ shoots on an MS medium beneath LED diodes combining red and green light is a good option for the stimulation of shoot proliferation in this plant [27].  
The culture of callus and capillary roots can be applicable in a diversified multi-cascade production of secondary metabolites for industry. Biologically active compounds can be acquired without the usage of a whole plant. Production in a bioreactor is not limited by the biotic and abiotic factors involved with conventional cultivation. Callus was successfully induced on explants originating from stems through the application of a B5 (Gamborg medium) basic medium supplemented with 0.8 mg dm\({}^{-3}\) 2,4-D(2,4-dichlorophenoxyacetic acid), 0.5 mg dm\({}^{-3}\) NAA, or 2.0 mg dm\({}^{-3}\) BA [28]. The team achieved callus production on a B5 medium enriched with 0.8 mg dm\({}^{-3}\) 2,4-D after 15 days in darkness. Subsequently, in order to obtain a suspension culture, a callus of high looseness was separated, and transferred into a liquid medium, with an addition of 2.0 mg dm\({}^{-3}\) BA, 0.5 mg dm\({}^{-3}\) NAA, 0.8 mg dm\({}^{-3}\) 2,4-D, and 600 mg dm\({}^{-3}\) inositol [31]. The regeneration of callus was also achieved from young leaves cultured on a solidified MS medium supplemented with 1 mg dm\({}^{-3}\) 2,4-D and 0.1 mg dm\({}^{-3}\) kinetin. On explants from young leaves bred on an MS medium without phytohormes, tumor induction was carried out through transformation by _Agrobacteriumum tumefaciens_ strains A281 and B6S3. Hairy roots were induced with _Agrobacteriumum rhizenes_ strain A4. Tumor and capillary roots were maintained on a hormone-free MS medium [6].