## 2 Materials and Methods  
The objects of the study are destructor strains acquired from the Russian National Collection of Industrial Microorganisms National Bioresource Center (RNCIM BRC) SRC \"Kurchatov Institute\"--GosNIIGenetika: _Pseudomonas chlororapulis subsp urantiaca_ (B-3419) 128, _Pseudomonas oryzihubitans_ (B-12467) Tik3, _Pseudomonas pseudocalcaligenes_ (B-6783) VSB-10, _Pseudomonas balearica_ (B-12843) 1-2, _Pseudomonas mendocina_ (B-5330), and amphoteric surfactant cocamidopropylbetaine CAPB (30%), made in Italy. To determine the concentration that inhibits the growth of microorganisms, a macromethod was used. For this purpose, 8 solutions of surfactants were prepared. The concentration of each subsequent solution was half the previous one. The initial surfactant concentration was 0.7296 mol/dm\({}^{3}\). Next, a suspension based on a saline solution (0.9% NaCl solution) with a final concentration of 10\({}^{7}\)-10\({}^{8}\) CFU/mL was prepared for each microorganism. The number of colonies was determined using a DEN-1 densitometer by the turbidity of cell suspensions within the value of 0.5 McFarland units (1 \(\times\) 10\({}^{8}\) CFU/mL). A suspension of the destructor strain of 1 cm\({}^{3}\) was added to each test tube. After 24 h, light scattering was measured at a wavelength of 980 nm using a UV 1800 spectrophotometer (Shimadzu, Kyoto, Japan). The minimum inhibitory concentration (MIC) corresponds to the minimum content of surfactants in the solution, at which the growth of biomass is inhibited. With the aim of obtaining experimental data to construct a kinetic model of the process of biodegradation of the surfactant, glass flasks were prepared with a volume of 250 cm\({}^{3}\) sterile solutions of cocamidopropylbetaine in three concentrations (C\({}_{1}\) = 22.8 mmol/dm\({}^{3}\), C\({}_{2}\) = of 11.4 mmol/dm\({}^{3}\), C\({}_{3}\) = 5.7 mmol/dm\({}^{3}\)) for each of the five destructor strains. Then, suspensions of strains in the volume of 1 cm\({}^{3}\) were transplanted into glass flasks under sterile conditions. The microorganisms were cultured at 30 \({}^{\circ}\)C in the LSI-3016A/LSI-3016R shaker incubator (Daihan Labtech, Namyangju-si, South Korea). The degree of biodegradation was determined by measuring the residual concentration of surfactants in the solution. For this purpose, samples of each of the solutions were taken daily in a volume of 5 cm\({}^{3}\). The samples were cleaned of bacterial cells by centrifugation until a transparent solution was obtained. In parallel, a solution of the black T eriochrome indicator and a solution of a sodium-phosphate buffer (pH = 6.86) were prepared in accordance with the procedure [23]. Solutions of the following composition were prepared for measurements:  
(1) For solutions with a surfactant concentration C\({}_{1}\) = 22.8 mmol/dm\({}^{3}\), 5.0 cm\({}^{3}\) buffers and 1.0 cm\({}^{3}\) indicators were added to 5 cm\({}^{3}\) of the sample;  
(2) For solutions with a surfactant concentration C\({}_{2}\) = 11.4 mmol/dm\({}^{3}\), 5.5 cm\({}^{3}\) buffers and 0.5 cm\({}^{3}\) indicators were added to 5 cm\({}^{3}\) of the sample;  
(3) For solutions with a surfactant concentration C\({}_{3}\) = 5.7 mmol/dm\({}^{3}\), 5.75 cm\({}^{3}\) buffers and 0.25 cm\({}^{3}\) indicators were added to 5 cm\({}^{3}\) of the sample. The values of the optical density of the solutions were measured with a UV 1800 spectrophotometer (Shimadzu, Kyoto, Japan) at a wavelength of 659 nm in a cuvette with a light-absorbing layer thickness of 1 cm. The reaction order and the value of the reaction rate constant were determined by graphical and analytical methods. Mathematical data processing was carried out in the Excel program. The statistical analysis data are expressed as the average value \(\pm\) standard deviation of three parallel measurements.