## 3 Results
### Phylogenetic Evolutionary Analyses Based on the Interactive Transcriptome  
In the interactive transcriptome of _S. mycoparsaitia_, the transcript, Sm68082_c0_g1_i1, annotated to a biotrophy-associated secreted protein 2 was the most closely related to the plant endophytic _Podospora comata_ (Sordariomycetes, Sordariomyctidae, Sordariales, Chaetomiaceae, and Podospora), also a saprophytic fungus on herbivore dung [44], _Colletotrichum orchidophilum_ (Glomerellales and Ascomycota), and _Prialocphala scopiformis_ (Helotiales and Ascomycota), as shown in Figure 3.  
### Phylogenetic Evolutionary Analyses Based on the Interactive Transcriptome  
In the interactive transcriptome of _S. mycoparsaitia_, the transcript, Sm68082_c0_g1_i1, annotated to a biotrophy-associated secreted protein 2 was the most closely related to the plant endophytic _Podospora comata_ (Sordariomycetes, Sordariomyctidae, Sordariales, Chaetomiaceae, and Podospora), also a saprophytic fungus on herbivore dung [44], _Colletotrichum orchidophilum_ (Glomerellales and Ascomycota), and _Prialocphala scopiformis_ (Helotiales and Ascomycota), as shown in Figure 3.  
Figure 3: Phylogenetic tree of the transcript related to the biotrophy-associated secreted protein in _S. mycoparsaitia_. The branch support bootstrap values were obtained by 1000 replicates using the NJ method. Figure 2: Schematic representation of predicted localization of the ABC transporters and the biotrophy-associated secreted (BAS) protein in _S. mycoparsaitia_. In melanosporaceous _S. mycoparsitica_, the total number of (predicted) transcripts annotated to ABC transporters was 81. Among them, families of ABC-B (11 transcripts, 14%), ABC-C (18 transcripts, 22%), and ABC-G (19 transcripts, 23%) covered higher proportions than the other families belonging to ABC-A, ABC-F (2 transcripts), Aliphatic sulfonates importer (TC 3.A.1.17.2), BtuF, Ribose importer (TC 3.A.1.2.1), and unknown families. In fact, 11 transcripts for the multidrug resistance exporter (TC 3.A.1.201) subfamily of the ABC-B family were found along with 18 transcripts for the conjugate transporter (TC 3.A.1.208) subfamily of the ABC-C family, as well as 19 transcripts for the pleiotropic drug resistance (PDR) (TC 3.A.1.205) subfamily of the ABC-G family. Out of the total 81 transcripts, selected transcripts based on a relatively higher frequency and/or identity are presented in Figure 4, showing transcripts per million (TPM) values for 7, 14, 5, and 2 transcripts belonging to ABC-B, -C, -G, and -F families, respectively, and the detailed description follows. Figure 4: RNA-Seq heat map revealing relative gene expression of selected transcripts for four families of ABC transporters (ABC-B, -C, -G, and -F) in _S. mycoparsitica_ interacting with _Fusarium graminearum_ 3-ADON. Sm C1 and Sm C2 represent _S. mycoparsitica_ incubated for 1.5 and 3.5 days, respectively, without interaction with the host, whereas Sm T1 and Sm T2 represent _S. mycoparsitica_ interacting with the host for respective incubation times (1.5 and 3.5 days). The log\({}_{2}\) transformed transcripts per million (TPM) values were applied for comparisons of relative gene expression to depict the overexpressed genes. In the transcriptome of _S. mycoparsitica_, the transcript, Sm105692_c0_g1_i1, annotated to ABC transporter 1 (alternative name, siderophore biosynthesis cluster protein ABC1 and gene name, _ABC1_) showed high identity (E-value 1.5 \(\times\) 10\({}^{-31}\), Score 293, and 72.7% identity) to the _Ajellomyces capsulatus_ (an intracellular budding _yeast_) ABC transporter 1 (UniProt identifier: ABC1_AJECA) belonging to the multidrug resistance exporter (TC 3.A.1.201) subfamily, ABC-B family. The TPM value of the transcript, Sm105692_c0_g1_i1, annotated to the ABC transporter 1 was higher in the host-interactive sample (2.193904 TPM) than in the control (0.4872588 TPM) at 3.5 days; The control and the host-interactive samples at 1.5 days both had no expression of the transcript, as shown in Figure 4 (ABC-B).