Taken in adequate amounts (viable cell concentration of 10\({}^{6}\)-10\({}^{9}\) cfu/g), they have a beneficial effect on the functioning of the digestive system and related organs and systems [8; 9; 10]. Gram-positive lactic acid bacteria of the genus _Lactobacillus_, the genus _Bifidobacterium_, yeast of the species _Saccharomyces boulardi_, fungi of the genus _Aspergillus_, spore-forming bacteria of the genus _Bacillus_ are most frequently included in the composition of probiotic preparations. _Bacillus_ species and strains are of significant interest for the development of probiotic preparations. _Bacillus_ species are characterized by rapid growth, they exhibit competitive exclusion of pathogenic microorganisms, and their spores are resistant to the conditions of the gastrointestinal tract (low pH values and the presence of pepsin, pH = 8, and the presence of pancreatin, high concentrations of bile salts), protecting the host's immune system [11; 12]. _Bacillus_ sp. produce substances with antimicrobial activity of different chemical nature. Antimicrobial substances include peptides, amino sugars, phospholipids, etc. According to [PERSON], 2007 and [PERSON] et al., 2017 [13; 14], antimicrobial compounds produced by _Bacillus_ strains exhibit antifungal activity against phytopathogenic fungi. _Bacillus_ sp. also produce a wide range of peptides with antimicrobial activity against pathogenic bacteria and fungi [15]. In recent years, the issue of aflat in food and protecting the population from their carcinogenic effect has been raised. The development of preparations with high antifungal activity against _Aspergillus flavus_ would allow both soil and plant individuals to be treated. This in turn would enable the production of safe foods. _Sclerotinia_ sp. (_Sclerotinia scleroorum_) affects the root system of plants and manifests itself as a wet light brown rotting spot at the base of the stem, which is later covered with a white cotton-like coating. Regardless of the infection phase, plant species die [16; 17]. _Bacillus_ sp. peptides are classified as ribosomally synthesized bacterios and polykeptides and lipopolysaccharide produced by non-ribosomal enzymes. Bacterios are able to act on the cytoplasmic membrane, increasing its semi-permeability and causing depolarization of the cell membrane of pathogenic bacteria. Some bacterios suppress microbial cell viability and cell division processes [18]. Polykeptides are secondary metabolites composed of fatty acids with high antifungal activity. They act on pathogenic microorganisms by repressing genes related to cell wall synthesis and DNA replication [19], and some block the synthesis of a number of proteins [20]. The best-studied bacterios are lipopolysaccharide, which are synthesized using peptide synthetase enzymes [21], and consist of a hydrophilic peptide part (consisting of 7 to 10 amino acids) connected to a hydrophobic fatty tail [22]. Depending on their structure, they form three groups: biosurfactants, iturins, and fengycins. Biosurfactants act on the lipid bilayer of various biological membranes [22]. Iturins possess high polymorphism with diverse biological, physiological, and biochemical properties [22]. Fengicides exhibit specific antifungal activity [22]. _Bacillus amyloIquefaciens_ is a species of the genus _Bacillus_ that secretes extracellular enzymes, such as \(\beta\)-amylases, cellulase, metalloproteinases, and proteases, through which it improves digestion, nutrient absorption, and intestinal resistance to infection [23]. _Bacillus amyloIquefaciens_ LFB112 secreted bacterios with good thermostability and acid/alkaline tolerance, active against a wide range of pathogenic bacteria--Gram-positive and Gram-negative bacteria, causing various host diseases. Microelements, which play an important role in the enzymatic reactions occurring in the cell and in the sporulation process, are necessary for the normal growth and development of bacilli. The bacilli cell walls are cation-selective.