## 3 Results and Discussion
### Unsupervised PCA Data Analysis of C. sativus and L. Siceraria Primary Metabolites  
To provide insight into _C. sativus_ and _L. Siceraria_ fruits' primary metabolome mediating for their nutritive value, GC-MS was employed, leading to the detection of 49 peaks. Chromatograms displayed a representative profile of _C. sativus_ and _L. Siceraria_ fruits' nutrient primary metabolites (Figure S15). Metabolites were categorized into alcohols (6), amino acids (2), fatty acids/esters (12), nitrogenous compounds (1), organic acids (12), phenolic acids (1), steroids (2), sugars (9), sugar acids (1), and sugar alcohols (3), as shown in Table 2. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Peak** & **Rt (min)** & **KI** & **Metabolite** & **M. formula** & _C. sativus_ & _L. Siceraria_ \\ \hline
1 & 5.31 & 987 & Ethylene glycol, 2 TMS derivative & C\({}_{8}\)H\({}_{22}\)O\({}_{5}\)Si\({}_{2}\) & 1.39 \(\pm\) 0.37 & 2.44 \(\pm\) 1.19 \\
2 & 5.60 & 1003 & Propylene glycol, 2 TMS derivative & C\({}_{6}\)H\({}_{24}\)O\({}_{5}\)Si\({}_{2}\) & 0.11 \(\pm\) 0.03 & 0.18 \(\pm\) 0.08 \\
4 & 6.62 & 1061 & 1,3 Propenedi di-TMS & C\({}_{9}\)H\({}_{24}\)O\({}_{5}\)Si\({}_{2}\) & 0.21 \(\pm\) 0.06 & 0.40 \(\pm\) 0.17 \\
7 & 8.23 & 1151 & 1,4-Butanediol, 2 TMS derivative & C\({}_{10}\)H\({}_{26}\)O\({}_{5}\)Si\({}_{2}\) & 0.12 \(\pm\) 0.03 & 0.18 \(\pm\) 0.10 \\
11 & 9.89 & 1251 & Triethylene glycol, 2 TMS derivative & C\({}_{12}\)H\({}_{30}\)O\({}_{4}\)Si\({}_{2}\) & 0.49 \(\pm\) 0.14 & 0.89 \(\pm\) 0.39 \\
13 & 10.41 & 1285 & Glycerol, 3 TMS derivative & C\({}_{12}\)H\({}_{30}\)O\({}_{5}\)Si\({}_{3}\) & 0.85 \(\pm\) 0.20 & 0.86 \(\pm\) 0.30 \\ \cline{3-6}  & & & **Total alcohols** & & **3.18 \(\pm\) 0.82** & **4.95 \(\pm\) 2.23** \\ \hline
19 & 12.19 & 1401 & Glycine, 3 TMS derivative & C\({}_{11}\)H\({}_{29}\)NO\({}_{2}\)Si\({}_{3}\) & 1.64 \(\pm\) 0.27 & 2.41 \(\pm\) 0.98 \\
20 & 12.63 & 1434 & \(\beta\)-Alanine, 3 TMS derivative & C\({}_{12}\)H\({}_{31}\)NO\({}_{2}\)Si\({}_{3}\) & 0.37 \(\pm\) 0.08 & 0.69 \(\pm\) 0.29 \\ \cline{3-6}  & & & **Total amino acids** & & **2.01 \(\pm\) 0.35** & **3.10 \(\pm\) 1.28** \\ \hline \multicolumn{6}{c}{**Fatty acids/esters**} \\
18 & 11.57 & 1360 & Caprylic acid n-butyl ester & C\({}_{12}\)H\({}_{24}\)O\({}_{4}\) & 5.70 \(\pm\) 1.64 & 9.74 \(\pm\) 4.49 \\
33 & 18.77 & 1948 & 12-Methyltetradacanoic acid TMS ester & C\({}_{18}\)H\({}_{38}\)O\({}_{2}\)Si & 0.38 \(\pm\) 0.18 & 0.14 \(\pm\) 0.09 \\
35 & 19.76 & 2045 & Palmitic Acid, TMS derivative & C\({}_{19}\)H\({}_{40}\)O\({}_{2}\)Si & 3.18 \(\pm\) 1.04 & 10.76 \(\pm\) 10.50 \\
37 & 20.72 & 2144 & Margaric acid, TMS ester & C\({}_{20}\)H\({}_{42}\)O\({}_{2}\)Si & 0.13 \(\pm\) 0.03 & 0.35 \(\pm\) 0.15 \\
38 & 21.38 & 2214 & Linoleic acid TMS ester & C\({}_{21}\)H\({}_{40}\)O\({}_{2}\)Si & 0.40 \(\pm\) 0.26 & 1.02 \(\pm\) 0.94 \\
39 & 21.42 & 2218 & Oleic acid, TMS ester & C\({}_{20}\)H\({}_{40}\)O\({}_{2}\)Si & 0.93 \(\pm\) 0.38 & 3.34 \(\pm\) 3.76 \\
40 & 21.46 & 2223 & \(\alpha\)-Linoleic acid, TMS & C\({}_{21}\)H\({}_{38}\)O\({}_{2}\)Si & 0.77 \(\pm\) 0.56 & 2.53 \(\pm\) 3.14 \\
41 & 21.63 & 2242 & Stearic acid, TMS derivative & C\({}_{21}\)H\({}_{40}\)O\({}_{2}\)Si & 2.78 \(\pm\) 0.71 & 6.51 \(\pm\) 3.11 \\
42 & 23.36 & 2440 & Arachidic acid, TMS derivative & C\({}_{23}\)H\({}_{48}\)O\({}_{2}\)Si & 0.04 \(\pm\) 0.03 & 0.09 \(\pm\) 0.03 \\
43 & 24.62 & 2600 & 1-Monopalmitin 2 TMS ether & C\({}_{25}\)H\({}_{54}\)O\({}_{4}\)Si\({}_{2}\) & 0.35 \(\pm\) 0.15 & 0.84 \(\pm\) 0.84 \\
46 & 26.08 & 2786 & Glycerol monosterarate, 2 TMS derivative & C\({}_{27}\)H\({}_{58}\)O\({}_{4}\)Si\({}_{2}\) & 0.26 \(\pm\) 0.12 & 0.39 \(\pm\) 0.27 \\
47 & 26.30 & 2812 & Sebacic acid, bis(2-ethylhexyl) ester & C\({}_{26}\)H\({}_{50}\)O\({}_{4}\)Si\({}_{2}\) & 0.38 \(\pm\) 0.11 & 0.66 \(\pm\) 0.29 \\ \cline{3-6}  & & & **Total fatty acids/esters** & & **15.32 \(\pm\) 5.19** & **36.38 \(\pm\) 27.62** \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Primary metabolite analyzed using GC-MS of different _C. sativus_ and _L.