# Morphological, Anatomical, and Phytochemical Studies of _[PERSON]_ [PERSON]. [PERSON]
## 2 Results and Discussion
### Fatty Acid Content
\begin{table}
\begin{tabular}{c c c c c c} \hline
**Compound Name** & **FA Abbreviation** & **RT FAME (min)** & **Mass Data FAME** & **Content of FA** \\ \hline Myristic acid & C14.0 & 11.09 \(\pm\) 0.1 & 242(M), 211, 199, 185, 166, 157, 143, 129, 115, 97, 87, & 0.07 \(\pm\) 0.10 \({}^{\ast}\) \\  & & 83, 74, 55 & & 0.07 \(\pm\) 0.10 \({}^{\ast}\) \\ Palmitic acid & C16.0 & 12.98 \(\pm\) 0.1 & 270(M), 255, 239, 227, 213, 199, 185, 171, 157, 143, & \\  & & 129, 115, 97, 87, 74, 69, 55 & & 7.18 \(\pm\) 0.20 \({}^{\ast}\) \\ cis-5-hexadecenoic acid & C16.1 (n-11) & 13.33 \(\pm\) 0.1 & 283, 268(M), 250, 236, 219, 194, 165, 152, 123, 110, 96, & 0.86 \(\pm\) 0.10 \({}^{\ast}\) \\  & & 84, 74, 67, 55 & & 0.05 \(\pm\) 0.10 \({}^{\ast}\) \\ Margaric acid & C17.0 & 13.99 \(\pm\) 0.1 & 313, 284(M), 253, 241, 199, 143, 101, 87, 74, 69, 55 & 0.05 \(\pm\) 0.10 \({}^{\ast}\) \\  & & 298(M), 267, 255, 241, 199, 157, 143, 129, 111, 97, 87, & 2.92 \(\pm\) 0.05 \({}^{\ast}\) \\  & & 74, 69, 55 & & \\ Unidentified compound & - & 15.49 \(\pm\) 0.2 & 264, 247, 222, 207, 180, 166, 177, 110, 96, 84, 74, 67, 55 & 0.16 \(\pm\) 0.10 \({}^{\ast}\) \\
5-Octadeconic acid & C18.1 (n-13) & 15.58 \(\pm\) 0.2 & 296(M), 278, 264, 247, 235, 222, 207, 194, 180, 166, & 18.66 \(\pm\) 0.03 \({}^{\ast}\) \\  & & 152, 173, 125, 129, 96, 84, 74, 67, 55 & & \\ Oleic acid & C18.1 (n-9) & 15.81 \(\pm\) 0.2 & 313, 296(M), 278, 264, 235, 222, 193, 180, 166, 110, 97 & 4.94 \(\pm\) 0.15 \({}^{\ast}\) \\  & & 83, 69, 55 & & \\
8-Octadecenoic acid \({}^{\ast}\) & C18.1 (n-10) & 15.92 \(\pm\) 0.2 & 313, 296(M), 264, 235, 222, 207, 180, 166, 138, 123, & 0.32 \(\pm\) 0.00 \({}^{\ast}\) \\  & & 111, 97, 83, 69, 55 & & \\
9,12-Octadecadiene acid & C18.2 (n-6) & 16.34 \(\pm\) 0.2 & 294(M), 263, 220, 150, 95, 67, 55 & 0.09 \(\pm\) 0.00 \({}^{\ast}\) \\  & & 294(M), 263, 225, 233, 200, 205, 191, 178, 164, 150, & 53.2 \(\pm\) 1.07 \({}^{\ast}\) \\  & & 235, 123, 109, 95, 81, 67, 59, & & \\ Arachidic acid & C20.0 & 17.96 \(\pm\) 0.2 & 326(M), 295, 283, 269, 255, 241, 227, 198, 185, 171, & 0.83 \(\pm\) 0.05 \({}^{\ast}\) \\  & & 149, 143, 137, 129, 115, 97, 87, 83, 74, 69, 55 & & \\ alpha-Linoleic acid & C18.3 (n-3) & 18.62 \(\pm\) 0.2 & 292(M), 250, 208, 179, 163, 149, 121, 108, 95, 79, 67, 55 & 0.58 \(\pm\) 0.00 \({}^{\ast}\) \\
11,14,17-Eicosadenoic acid \({}^{\rm b}\) & C20.3 (n-3) & 18.98 \(\pm\) 0.2 & 292, 250, 208, 179, 163, 149, 121, 108, 95, 79, 67, 55 & 0.12 \(\pm\) 0.00 \({}^{\ast}\) \\  & Magnificic acid & C18.2 (n-3) & 19.30 \(\pm\) 0.2 & 294(M), 263, 193, 164, 150, 123, 109,Our study showed that the content of linolenic, 5-octadecenoic, stearic, palmitic, and oleic acids in the tested oil was 53.21, 18.66, 2.92, 7.18, and 4.94%, respectively, which is in agreement with the literature data [13]. Additionally, an unknown compound was visible in the chromatogram with a retention time of 13.3 min, which was probably cis-5-hexadecenoic acid, as suggested by the mass of the molecular ion and literature [13]. Our research showed that _C. acaulis_ oil also contained myristic, palmitoleic, margaric, 8-octadecenoic, 9,12-octadecenoic, arachidic, alpha-linolenic, 11,14,17-eicosatrienoic, and mangiferic acids and one unidentified compound (retention time 15.49 min). The differences between our findings and [PERSON]'s report [13] are probably the results of the use of different analytical techniques. It should be noted that [PERSON] et al. used gas chromatography with flame ionization detection and thin-layer chromatography in their research. Both these techniques carry a higher risk of misidentification of analytes, compared to the GC-MS used this study. Moreover, the analytical columns used in 1969 did not have such high resolution as those used today. This study therefore verifies the results of [PERSON] et al. and enriches the knowledge of the composition of _C. acaulis_ cyspela oil with new data. The high content of linoleic acid in the tested oil suggests its high pro-health value. Linoleic acid is a polyunsaturated fatty acid belonging to the omega-6 group.