## 2 Results
### In Vivo Assays of VOC  
Of the two in vivo trials conducted for VOC production, the results of the first BCAs in a Petri dish underneath grapes inoculated with pathogens (Scheme 1b) showed significant differences in mycelial growth of all pathogens, for the three grape cultivars evaluated, compared to control (Figure 2). For cv. Red Globe, the most significant effect was against _A. tubingensis_, at 93% mycelial inhibition (Table 2). There, _G. cerinus_ was shown to inhibit its sporulation as well. Similar results were observed for cv. Thompson Seedless, at 79% mycelial inhibition for _B. cinerea_ (statistically significant, Table 2). For cv. Crimson Seedless, the PUCV-VBL biocontrol VOCs presented the same level of significance against _A. tubingensis, P. expansum_ and _R. stolonifer_. Only for _R. stolonifer_ was the effect slightly less (Table 2). Figure 2: In vivo test berries inoculated with pathogens on Petri dishes with biocontrol. Effect of VOCs produced by (1) control treatment without biocontrol; (2) _H. osmophila_ biocontrol; (3) _G. cerinus_ biocontrol; (4) Biological consortium PUCV-VBL biocontrol. (**a**) Percentage of mycelial inhibition of _B. cinerea_ in cv Red Globe, (**b**) percentage of mycelial inhibition of _A. tubingensis_ in cv Red Globe, (**c**) percentage of mycelial inhibition of _P. expansum_ in cv Red Globe, (**d**) percentage of mycelial inhibition of _R. stolonifer_ in cv Red Globe, (**e**) percentage of mycelial inhibition of _B. cinerea_ in cv Thompson Seedless, (**f**) percentage of mycelial inhibition of _A. tubingensis_ in cv Thompson Seedless, (**g**) percentage of mycelial inhibition of _P. expansum_ in cv Thompson Seedless, (**h**) percentage of mycelial inhibition of _R. stolonifer_ in cv Thompson Seedless, (**i**) percentage of mycelial inhibition of _B. cinera_ in cv Crimson Seedless, (**j**) percentage of mycelial inhibition of _A. tubingensis_ in cv Crimson Seedless, (**k**) percentage of mycelial inhibition of _P. expansum_ in cv Crimson Seedless, (**l**) percentage of mycelial inhibition of _R. stolonifer_ in cv Crimson Seedless. [PERSON]’s multiple comparisons test \({}^{*}\)_p_ < 0.05; **\({}^{**}\)_p_ < 0.01; **\({}^{**}\)_p_ < 0.001; **\({}^{***}\)_p_ < 0.0001. The second in vivo trial results--BCAs in grapes placed underneath pathogen-inoculatedberries (Scheme 1c)--showed significant differences for _B. cinerea_ and _P. expansum_ in cv. Red Globe (Figure 3a,c); and, for cvs. Thompson Seedless and Crimson Seedless, all treatments differed from control for all the pathogens evaluated (Figure 3e-l). \begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{2}{*}{**Cultivar**} & \multirow{2}{*}{**Pathogen**} & \multicolumn{3}{c}{**Biocontrol Agents (BCAs)**} \\ \cline{3-5}  & & _G. cerimus_ & _H. osmophila_ & **PUCV-VBL** \\ \hline \multirow{4}{*}{Red Globe} & _B. cinerea_ & 82 \(\pm\) 9 a  & 76 \(\pm\) 14 a  & 79 \(\pm\) 15 a  \\  & _A. tubingensis_ & 75 \(\pm\) 22 a,b  & 68 \(\pm\) 23 a  & 93 \(\pm\) 11 b  \\  & _P. expansum_ & 71 \(\pm\) 12 a  & 66 \(\pm\) 14 a  & 78 \(\pm\) 12 a  \\  & _R. stolonifer_ & 82 \(\pm\) 12 a  & 73 \(\pm\) 10 a  & 84 \(\pm\) 15 a  \\ \hline \multirow{4}{*}{Thompson seedless} & _B. cinerea_ & 54 \(\pm\) 22 a  & 75 \(\pm\) 11 b  & 79 \(\pm\) 8 b  \\  & _A. tubingensis_ & 77 \(\pm\) 16 a  & 73 \(\pm\) 7 a  & 71 \(\pm\) 13 a  \\  & _P. expansum_ & 77 \(\pm\) 22 a  & 85 \(\pm\) 2 a  \\  & _R. stolonifer_ & 68 \(\pm\) 24 a  & 71 \(\pm\) 22 a  \\ \hline \multirow{4}{*}{Crimson seedless} & _B. cinerea_ & 82 \(\pm\) 9 b  & 54 \(\pm\) 21 a  & 86 \(\pm\) 12 b  \\  & _A. tubingensis_ & 64 \(\pm\) 21 a,b  & 57 \(\pm\) 18 a  & 75 \(\pm\) 14 b  \\  & _P. expansum_ & 50 \(\pm\) 11 a  & 49 \(\pm\) 15 a  \\  & _R. stolonifer_ & 89 \(\pm\) 13 b  & 60 \(\pm\) 20 a  & 64 \(\pm\) 14 a  \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Percentage of inhibition of mycelial growth in vitro of different causal agents with grape-plate test.