## 3 Results
### Antimicrobial Activity of Anthocyanins  
The results of the research show that anthocyanins of _Aronia melanocarpa_ and _Vaccinium myrtillus_ display antimicrobial activity against clinical and reference strains of _S.aureus_. Only _Vaccinium myrtillus (acetone)_ was shown to exert antimicrobial activity against reference and clinical strains of _E. faecalis_. Antimycotic activity of anthocyanins was not revealed. Absence of antimicrobial activity of anthocyanins against _S. pyogenes_ and clinical strains of _E. coli_ was observed. It was further demonstrated that anthocyanins of _Sambucus nigra_ did not show any antimicrobial activity. It was proved that _Vaccinium myrtillus (acetone)_ displays antimicrobial activity against clinical and reference strains of _S. aureus_ and _E. faecalis_. The antimicrobial activities of extracts against typical and clinical strains were given at Tables 6 and 7 respectively. \begin{table}
\begin{tabular}{c c c c c c c} \hline \multirow{2}{*}{**N**} & \multirow{2}{*}{**Samples**} & _S. aureus_ & _E. coli_ & _E. faecalis_ & _S. pyogenes_ & _C. albicans_ \\  & & _ATCC_ & _ATCC_ & _ATCC_ & _ATCC_ \\  & & 25923 & 25922 & 29212 & 19615 & 885-653 \\ \hline \multirow{2}{*}{1} & _Vaccinium myrtillus_ (ethanol) & 9.33 \(\pm\) 0.50 & - & - & - & - \\  & & & & & & \\ \hline \multirow{2}{*}{2} & _Vaccinium myrtillus_ (acetone) & 9.50 \(\pm\) 0.50 & 8.66 \(\pm\) 0.58 & 17.66 \(\pm\) 0.58 & - & \\ \hline \multirow{2}{*}{3} & _Aronia melanocarpa_ (ethanol) & 16.50 \(\pm\) 0.29 & 8.33 \(\pm\) 0.58 & - & - & \\ \hline \multirow{2}{*}{4} & _Aronia melanocarpa_ (acetone) & 13.33 \(\pm\) 0.58 & - & 12.83 \(\pm\) 0.58 & - & - \\ \hline \multirow{2}{*}{5} & _Sambucus nigra_ (ethanol) & - & - & - & - & - \\ \hline \multirow{2}{*}{6} & _Sambucus nigra_ (acetone) & - & - & - & - & - \\ \hline \multirow{2}{*}{7} & _Vaccinium corymbosum_ (ethanol) & - & 10.83 \(\pm\) 0.76 & - & - & - \\ \hline \end{tabular}
\end{table} 
Table 6: Antimicrobial activity of anthocyanins against typical strains, zones inhibition in millimeters including diameter of well, mm (n = 3, x \(\pm\) SD). ( – : no inhibition). \begin{table}
\begin{tabular}{c c c c c c c c} \hline \multirow{2}{*}{**IC\({}_{50}\)**} & **RSNA** & **RACA** & **RVMA** & **RACE** & **RSNE** & **RVME** & **RVCE** \\ \hline \multirow{2}{*}{24 h} & up to & 0.88 & \multirow{2}{*}{0.108 mg/mL} & \multirow{2}{*}{0.108 mg/mL} & \multirow{2}{*}{0.370 mg/mL} & \multirow{2}{*}{0.175 mg/mL} & \multirow{2}{*}{0.196 mg/mL} \\  & 1 mg/mL & mg/mL & & & & & \\ \hline \multirow{2}{*}{48 h} & up to & \multirow{2}{*}{0.541 mg/mL} & \multirow{2}{*}{0.576 mg/mL} & \multirow{2}{*}{0.093} & \multirow{2}{*}{0.151 mg/mL} & \multirow{2}{*}{0.159 mg/mL} & \multirow{2}{*}{0.167 mg/mL} \\  & 1 mg/mL & & & & mg/mL & & \\ \hline \end{tabular}  
* Indications: RSNE: _Sambucus nigra_ ethanol, RSNA: _Sambucus nigra_ acetone; RVME: _Vaccinium myrtillus_ ethanol; RVMA: _Vaccinium myrtillus_ acetone; RACE: _Aronia melanocarpa_ ethanol, RACA: _Aronia melanocarpa_ acetone and RVCE: _Vaccinium coryosum_ ethanol.  
\end{table} 
Table 5: The IC\({}_{50}\) doses of anthocyanins on DLD1 Cells. The MIC and the MBC of _Vaccinium myrtillus_ (ethanol) against clinical and reference isolates of _S. aureus_ equaled to 15.0 and 20.0 mg/mL, respectively. The antimicrobial activity by MIC (2.5 mg/mL) and MBC (5.0 mg/mL) indices of _Vaccinium myrtillus_ (acetone) that was determined against the reference _E. faecalis_ ATCC 29212, equaled to 10.0 and 15.0 mg/mL, respectively, against the clinical isolates of _Enterococci_. The antimicrobial activity of _Vaccinium myrtillus_ (acetone) was observed upon _staphylococcus_ isolates: MIC was 10.0-15.0 mg/mL, and MBC was 20.0 mg/mL. No bactericidal effect of this extract upon the other isolates was observed. MIC against typical _E. coli_ equaled to 25.0 mg/mL. _Aronia melanocarpa_ (ethanol) exerted a bactericidal effect upon clinical isolates of _S. aureus_ (MBC was 10.0 mg/mL, and MIC was 5.0 mg/mL).