###### Abstract  
Table grapes (_Vitis vinifera_) are affected by botrytis bunch rot and summer bunch rot, the latter a complex disease caused by _Botrytis cinerea_, _Aseggillus_ spp., _Penicillium expansum_ and _Rhizopus stolonifer_. To search for biocontrol alternatives, a new bioproduct composed of _Gluconobacter ceriumus_ and _Hansenispora osmophila_, a consortium called PUCV-VBL, was developed for the control of fungal rots in table grapes. Since this consortium presents new biocontrol species, the effect of their VOCs (volatile organic compounds) was evaluated under in vitro and in vivo conditions. The VOCs produced by the PUCV-VBL consortium showed the highest mycelial inhibition against _Botrytis cinerea_ (86%). Furthermore, _H. osmophila_ was able to inhibit sporulation of _A. tubingensis_ and _P. expansum_. VOCs' effect in vivo was evaluated using berries from Red Globe, Thompson Seedless and Crimson Seedless grapes cultivars, demonstrating a mycelial inhibition by VOCs greater than 70% for all evaluated fungal species. The VOC identification of the PUCV-VBL consortium was analyzed by solid-phase microextraction coupled to gas chromatography-mass spectrometry (SPME-GCMS). A total 26 compounds were identified, including 1-butanol 3-methyl, propanoic acid ethyl ester, ethyl acetate, phenylethyl alcohol, isobutyl acetate and hexanoic acid ethyl ester. Our results show that VOCs are an important mode of action of the PUCV-VBL biological consortium. bioantagonist; volatile organic compounds; table grapes; mycelial inhibition; biocontrol; summer bunch rot +
Footnote †: journal: _Antibiotics_  
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Footnote 0: _E-mail:_  
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Footnote 0: _E-mail:_  
_expansum_[4]. These pathogens are distributed worldwide, and infection can occur during the growing season, harvest, postharvest, storage, or transport [5; 6]. Especially in postharvest, these diseases can cause significant economic losses: it is estimated that, in developed countries, 25% of fruit can be affected by such pathogens; and that, in developing countries, the percentage doubles [7], accounting for 80% of postharvest losses [8]. Chile, which is the most important table grape exporting country globally [9], these diseases can cause severe difficulties, especially when reaching the Far East destination market. In this context, fungicides are the most common control method for these pathogens [10]; indeed, table grape and wine sectors represent 50% of the total market value for fungicides [10]. However, fungicide resistance is a growing problem [11; 12; 13; 14; 15]. Botrytis management's overall expenses, including cultural measures, botrycides, broad-spectrum fungicides, and biocontrols, easily reach \(\xi\)1 billion/year in all countries; product and quality loss in the market chain, however, is probably much higher [10]. While Regulation (EU) 2015/408 [16] has proposed a chemical substitution list currently used in traditional agriculture, these alternative methods still require development and evaluation to demonstrate at least the same effectiveness in controlling postharvest diseases. With this goal, physical, chemical, and biological (biocontrol) control methods have been developed as alternatives to pesticides [14]. Of the above, biocontrol methods seek to decrease microbial pathogen inoculum or remit disease through one or more yeast, bacterial, and or fungal microorganisms [17]. Over the past 30 years, biocontrol research by multidisciplinary scientific teams, agri-food companies in the agri-sector and multinationals chemical companies [18] has produced phytosanitary compounds and contributed to its becoming an effective strategy to combat postharvest decay of fruits. Research into, e.g., yeast biocontrol methods have shown promising results, demonstrating ideal antagonistic properties and adaptability to adverse environmental conditions, few nutritional requirements, and prolonged half-life formulation [19; 20].