The approved therapeutics to combat aspergillosis include three types of antifungal agents, namely polyenes, triazoles, and echinocandins. However, the continued use of triazole agents against _Aspergillus_ spp. has led to a greater occurrence of triazole-resistant strains. Studies suggest that triazole-resistant IA infection is significantly associated with treatment failure, with a mortality rate of 30-90%, depending on the patient type [16]. Similarly, voriconazole resistance has also been reported to be associated with an overall mortality of 21-25% in patients infected with IA [16]. However, relatively little is known about the amphotericin B susceptibility patterns of _A. fumigatus_ in many parts of the world. Therefore, it is important to discover or develop new antifungal therapeutics as substitutes to conventional drugs to counter life-threatening fungal infections. Nanotechnology offers tools to treat communicable and non-communicable diseases via novel mechanisms. For example, many researchers have considered the use of nanoparticles as drug carriers. The nanoparticles are straightforward to manipulate; their properties (e.g., size, shape, and surface chemistry) make these materials versatile platforms for treating bacterial and fungal infections [17; 18]. In addition, owing to their high drug-loading efficiency and ability to cross biological membranes, selectively surface-tuned nanoparticles may serve as antimicrobial drug carriers for delivering drugs at the site of infection [19; 20]. The capability of nanoparticles to interact with bacterial cells depends on the surface charge of the engineered nanoparticles; appropriate consideration of this parameter can increase the efficacy of the nano system against the pathogen. Various metal nanoparticles, such as silver, gold, zinc oxide, titanium dioxide aluminum oxide, and copper oxide nanoparticles, have been rigorously evaluated, either alone or conjugated with antibiotics and other functional molecules, for their potential to treat bacterial and fungal infections [21; 22]. Among these, AuNPs have drawn significant interest because of their low cellular toxicity, attractive optical properties, and straightforward functionalization with appropriate drug molecules [23]. Recent studies have evaluated utilizing AuNPs as drug carriers for antimicrobial agents [24; 25]. AuNPs can efficiently deliver relatively high drug concentrations at the site of infection with negligible cytotoxicity. For example, [PERSON] et al. noted that the combination of AuNPs with ciprofloxacin or cefotaxime provided synergistic activity against _Salmonella_ species through the induction of bacterial apoptosis-like phenomena [26]. Similarly, [PERSON] et al. noted that colistin-conjugated AuNPs significantly enhanced the antibacterial efficacy of colistin against _E. coli_, providing a six-fold reduction in the minimum inhibitory concentration value when compared to colistin alone [27]. Furthermore, several studies have been conducted to increase the effectiveness of antifungal drugs by loading AuNPs, and these structures have potential uses in treating fungal infections. Several approaches have been developed to counter antifungal drug resistance in fungal strains by conjugating drugs or small molecules to enhance their killing efficiency. For example, indolicidin was conjugated with gold nanoparticles, and its efficacy was evaluated using fluonazole-resistant clinical isolates of _C. albicans_[28]. Vancomycin has a unique mode of action, which involves the inhibition of the second stage associated with cell wall synthesis in susceptible bacteria [29]. The inter-domain exploration of the antifungal activity of classical antibacterial drugs has not been considered. However, one study considered vancomycin as a biofilm inhibitor in synergy with other antibiotics against mature _Candidida_ biofilms [30]. The present work explores the synthesis of water-dispersible gold nanoparticles functionalized with vancomycin and evaluates their in vitro antifungal activity against _A. fumigatus_ and _A.