## 1 Introduction  
In biosensor systems, spiral-coiled communications with a flowing aqueous medium are often used for thermal stabilization. Such communications are employed both in miniaturized biosensor systems and in large bioreactors [1]. Upon the flowing of water through spiral-wound communications, triboelectric effects occur [2; 3; 4; 5; 6]; the occurrence of such effects leads to the generation of an electric current. In turn, the motion of electric charges (i.e., the electric current) is known to induce electromagnetic fields. Despite these fields being weak, they nevertheless can influence biological objects. As is known, weak electromagnetic radiation, whose power is in the range of \(\sim\)10\({}^{-4}\) W, has an influence on human body (though this influence is weak)--so-called non-thermal effects [7; 8]. Studying these effects is of great importance, since weak radiation is beginning to be used in medicine for disease treatment. For instance, pilot research is being undertaken to study the therapeutic effect of electromagnetic radiation of non-thermal power in cancer [7]. At the same time, the influence of weak electromagnetic radiation on enzyme systems is still insufficiently studied. So, a number of studies have concerned the aggregation state of proteins in biosensor systems [9; 10], but the influence of inductive electromagnetic fields was not considered. On the other hand, it was demonstrated that a triboelectric effect appears upon flowing of water in polymeric pipes, which leads to a generation of charge [2; 3; 4; 5; 6]; accordingly, an electromagnetic field is induced. In modern biosensors, polymeric materials are widely used in fluidic communications, in thermostabilization systems etc. Recent experimental studies showed that the triboelectric effect is observed not only for pure (deionized or distilled) water, but also for tap water [11; 12; 13] and even for 0.6 M NaCl and seawater [11; 12], whose resistivity is low. At that, obviously, biosensors are fabricated in the form of compact devices, to save materials, working space etc. Since an electric field is induced upon the flowing of a liquid through communication components, the influence of this field can spread beyond these components. Accordingly, it is of interest to find out the relationship between the process of the flowing of a liquid sample and the properties of the sample located, for instance, in the measuring cell. Thus, in our present study, typical conditions occurring in a biosensor or in a bioreactor have been modeled, and the influence of flowing of water through spiral communications of a biosensor on the properties of a protein, with an example of a well-studied protein--horseradish peroxidase (HRP) enzyme--has been investigated. HRP pertains to heme-containing enzymes [14]. These enzymes play an important role in various metabolic processes in human [15]. Moreover, HRP is often employed in biosensor analysis as an enzymatic tag [16; 17]. These are the reasons why this protein has been chosen as a model object for our study. In our present research, HRP has been employed as an object of study, since this protein is a well characterized enzyme often used as a model in studies of a wide class of peroxididases. The study of peroxididases is of great interest due to the fact that these enzymes are well represented in plant and animal tissues [18] and play an important functional role in the organism. Peroxidase catalyzes the oxidation of a broad spectrum of organic and inorganic compounds by hydrogen peroxide [19]. In particular, one should point out an important role of myeloperoxidase, which participates in atherogenesis in humans [20]. The molecular weight of HRP heme-containing glycoprotein is 40 to 44 kDa [21; 22]. It is known that many enzymes, including horseradish peroxidase [23], form aggregates. It was demonstrated that a change in the aggregation state of HRP is observed upon the influence of electromagnetic fields with 100 Hz to 100 kHz frequencies.