## 3 Results and Discussion
### Proposed Identification of Chemicals in Avocado (P. americana Mill.) Semil 34 Seed
\begin{table}
\begin{tabular}{c c c c c c c c} \hline
**Peak** & **RT** & **Proposed Compound** & **Molecular** & **Ionization** & **\(m/z\) Observed** & **\(m/z\) Theoretical** & **Error** & **References** \\
**No.** & & **Identification** & **Formula** & **Ionization** & **Observed** & **Theoretical** & **Error** & **References** \\ \hline \multicolumn{8}{c}{Carboxylic acids and derivatives} \\ \hline
1 & 0.98 & gamma-Aminobutyric acid & C\({}_{4}\)H\({}_{8}\)NO\({}_{2}\) & [M+H]\({}^{+}\) & 104.0703 & 104.0706 & \(-\)2.8 & [51] \\ \hline
2 & 1.14 & Citric acid & C\({}_{6}\)H\({}_{8}\)O\({}_{7}\) & [M-H]\({}^{-}\) & 191.0188 & 191.0197 & \(-\)4.7 & [40; 47] \\ \hline
3 & 1.36 & Pantothenic acid & C\({}_{6}\)H\({}_{17}\)NO\({}_{5}\) & [M+H]\({}^{+}\) & 220.1178 & 220.1179 & \(-\)0.4 & [51] \\ \multicolumn{8}{c}{Cinnamic acid and derivatives} \\ \hline
4 & 1.64 & Neochlorogenic acid & C\({}_{16}\)H\({}_{18}\)O\({}_{9}\) & [M-H]\({}^{-}\) & 353.0873 & 353.0878 & \(-\)1.4 & [47] \\ \hline
5 & 2.85 & Caffe acid & C\({}_{9}\)H\({}_{8}\)O\({}_{4}\) & [M+H-H2O]\({}^{+}\) & 163.0387 & 163.0389 & \(-\)1.2 & [41] \\ \hline
6 & 2.85 & Caifeoylquinic acid & C\({}_{16}\)H\({}_{18}\)O\({}_{9}\) & [M+H]\({}^{+}\) & 355.1022 & 355.1023 & \(-\)0.3 & [20; 41; 48] \\ \hline
7 & 3.53 & 3-O-p-Coumaroylquinic acid & C\({}_{16}\)H\({}_{18}\)O\({}_{8}\) & [M-H]\({}^{-}\) & 337.0922 & 337.0929 & \(-\)2 & [18; 42] \\ \hline \multicolumn{8}{c}{Fatty acids} \\ \hline
8 & 15.92 & Methyl tetradeacanoate & C\({}_{15}\)H\({}_{30}\)O\({}_{2}\) & [M+H]\({}^{+}\) & 243.2316 & 243.2318 & \(-\)0.6 & [51] \\ \hline
9 & 17.60 & Methylenehexadecanoic acid & C\({}_{17}\)H\({}_{32}\)O\({}_{2}\) & [M+H-H2O]\({}^{+}\) & 251.2367 & 251.2369 & \(-\)0.7 & \\ \hline
10 & 27.45 & 2-Linoleoylglycerol & C\({}_{21}\)H\({}_{38}\)O\({}_{4}\) & [M+H-H2O]\({}^{+}\) & 337.2732 & 337.2737 & \(-\)1.4 & [52] \\ \hline \end{tabular}
\end{table} 
Table 2: Compounds identified from Semil 34 seed extract using HPLC-QTOF-MS/MS. \begin{table}
\begin{tabular}{c c c c c c c c c} \hline
**Peak** & **RT** & \begin{tabular}{c} **Proposed Compound** \\ **Identification** \\ \end{tabular}  & \begin{tabular}{c} **Molecular** \\ **Formula** \\ \end{tabular}  & **Ionization** & \begin{tabular}{c} _m/z_ \\ **Observed** \\ \end{tabular}  & \ \begin{tabular}{c} _m/z_ \\ **Theoretical** \\ \end{tabular}  & \begin{tabular}{c} **Error** \\ **(ppm)** \\ \end{tabular}  & **References** \\ \hline
11 & 28.32 & \begin{tabular}{c} 1-Palmitoyl-2-linoleoyl-rac- \\ glycerol \\ \end{tabular}  & C\({}_{37}\)H\({}_{68}\)O\({}_{5}\) & [M+H]\({}^{*}\) & 593.5131 & 593.5138 & \(-\)1.17 & [52] \\ \hline
12 & 20.39 & Monopalmitolein & C\({}_{19}\)H\({}_{36}\)O\({}_{4}\) & [M+H-H\({}_{2}\)O]\({}^{*}\) & 311.2569 & 311.2580 & \(-\)3.5 & \\ \hline
13 & 6.51 & \begin{tabular}{c} 3-Hydroxy-3- \\ methylglutaric acid \\ \end{tabular}  & C\({}_{6}\)H\({}_{10}\)O\({}_{5}\) & [M+H]\({}^{*}\) & 163.0599 & 163.0601 & \(-\)1.2 & [53] \\ \hline
14 & 21.99 & \begin{tabular}{c} Linolealidic acid \\ \end{tabular}  & C\({}_{18}\)H\({}_{32}\)O\({}_{2}\) & [M+H-H\({}_{2}\)O]\({}^{*}\) & 263.2367 & 263.2369 & \(-\)0.7 & \\ \hline
15 & 18.36 & Avocadenol A & C\({}_{17}\)H\({}_{32}\)O\({}_{3}\) & [M+H-3H\({}_{2}\)O]\({}^{*}\) & 231.2105 & 231.2107 & \(-\)0.8 & [54,55] \\ \hline
16 & 18.52 & 4-AcO-avocadyne & C\({}_{19}\)H\({}_{34}\)O\({}_{4}\) & [M+H-H\({}_{2}\)O]\({}^{*}\) & 309.2423 & 309.2424 & \(-\)0.3 & [54,55] \\ \hline
17 & 19.24 & Avocadene acetate & C\({}_{19}\)H\({}_{36}\)O\({}_{4}\) & [M+H-2H\({}_{2}\)O]\({}^{*}\) & 293.2472 & 293.2475 & \(-\)1.02 & [54,55,56] \\ \hline
18 & 13.58 & \begin{tabular}{c} 16-Heptadecyn-4-one, \\ 1,2-dihydroxy \\ \end{tabular}  & C\({}_{17}\)H\({}_{30}\)O\({}_{3}\) & [M+H-H\({}_{2}\)O]\({}^{*}\) & 265.2160 & 265.2162 & \(-\)0.7 & \\ \hline
19 & 18.03 & \begin{tabular}{c} 95,11E-Octadecidenic acid \\ \end{tabular}