## 4 Conclusions
In addition, the sequences used in this research are available on the NCBI server, BioProject accession: PRJNA522260 (ID: 522260) ([[https://www.ncbi.nlm.nih.gov/bioproject/PRJNA522260](https://www.ncbi.nlm.nih.gov/bioproject/PRJNA522260)]([https://www.ncbi.nlm.nih.gov/bioproject/PRJNA522260](https://www.ncbi.nlm.nih.gov/bioproject/PRJNA522260)), registration on 13 February 2019). Conceptualization, F.A.-C.; methodology, F.A.-C., C.G., S.P.-D., A.A.C. and S.C.; aDNA analysis, C.G. and S.P.-D.; formal data analysis and interpretation, F.A.-C., S.P.-D. and A.A.C. ; bioanthropological, archaeology, and archeothanatology data analysis, F.A.-C., S.C. and R.B.d.S. ; data curation, F.A.-C., A.A.C., S.P.-D. and S.C.; writing--original draft preparation, F.A.-C., S.P.-D., A.A.C., C.G., S.C., R.B.d.S. and E.A.-P.; writing--review and editing, F.A.-C., C.G., S.P.-D., A.A.C. and S.C.; visualization, F.A.-C.; project administration, F.A.-C.; funding acquisition, F.A.-C. All authors have read and agreed to the published version of the manuscript. **Funding:** This research was funded by the research projects: Bone Matters/Materias Oseas (reference: IF/00127/2014/CP1233/CT0003/) and Life After Death: Rethinking Human Remains and Human Osteological Collections as Cultural Heritage and Biobanks (reference: 2020.01014.CEECIND)--both projects are funded both by Fundacao para a Ciencia e Tecnologia (Portugal) and Coordinated by [PERSON]. [PERSON]'s research was also funded by Universidade NOVA FCSH 6\({}^{\circ}\) Edicao do Finanamento Exploratorio para Projetos. This research was within the scope of CRIA--Centro em Rede de Investigacao em Antropologia (reference UIDB/04038/2020) Strategic Plan and development of CRIA's laboratory profile via its Laboratory of Biological Anthropology and Human Osteology--LABOH, funded by Fundacao para a Ciencia e Tecnologia (Portugal). **Institutional Review Board Statement:** The study on human remains was conducted according to the American Association of Biological Anthropology ethical guidelines and the British Association for Biological Anthropology and Osteoarchaeology (BABAO) Code of Ethics and Code of Ethics Practice for the analysis and handling of human remains. Only limited samples were used and targeted specific analyses that were impossible to conduct in any other way. All data will be made available to prevent unnecessary resampling of the remains. **Informed Consent Statement:** Not applicable. **Data Availability Statement:** The sequences used in this research are available on the NCBI server, BioProject: PRJNA522260 (ID: 522260) ([[https://www.ncbi.nlm.nih.gov/bioproject/PRJNA522260](https://www.ncbi.nlm.nih.gov/bioproject/PRJNA522260)]([https://www.ncbi.nlm.nih.gov/bioproject/PRJNA522260](https://www.ncbi.nlm.nih.gov/bioproject/PRJNA522260)), registration on 13 February 2019). **Acknowledgments:** We would like to acknowledge the privilege to have had the opportunity to study the human remains that led to this research, further recognizing their contribution to the scientific development of the disciplines involved in this study as well as the historical context of the Hospital Real de Todos-os-Santos, in Lisbon, Portugal. We would also like to highlight the significance of human remains as a legacy from past populations and their importance as cultural heritage. **Conflicts of Interest:** The authors declare to have no conflict of interest.