###### Abstract  
Bacterial cellulose is a unique biopolymer that has found numerous biomedical applications, such as being an excellent wound-dressing material or a carrier for delivering active compounds. The purpose of this study was to analyze the ability of modified bacterial cellulose (BC) using low-pressure Ar plasma to control the release of glycoside hydrolaseses with antib biofilm activity, namely PelA\({}_{\text{h}}\) and PslG\({}_{\text{h}}\), from _Pseudomonas aeruginosa_. The chemical composition and morphology of the BC surfaces were characterized using photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The analyses revealed significant changes in the chemical composition of the BC surface due to the introduction of charged functional groups and the conversion of its well-ordered structure into a more amorphous form. The release profiles of enzymes from both forms of the carrier were different and depended on their structural properties. However, a significant impact of BC modification on protein release behavior from the carrier was observed only for PslG\({}_{\text{h}}\). Both enzymes, when immobilized on pristine and argon plasma-modified BC, retained their ability to effectively reduce biofilm levels, similarly to their soluble form. Ar plasma-modified BC with immobilized specific hydrolases can be used as an effective tool for inhibiting _P. aeruginosa_ biofilm development. bacterial cellulose; argon plasma; biofilm; surface modification; immobilization +
Footnote †: journal: Applied Sciences  
[PERSON], [PERSON], [PERSON], [PERSON], [PERSON], [PERSON], R. An Evaluation of the Usability of Argon Plasma-Treated Bacterial Cellulose as a Carrier for Controlled Releases of Biological Cellulose as a Carrier for Controlled Releases of Glycoside Hydrolaseses PelA\({}_{\text{h}}\) and PslG\({}_{\text{h}}\), Which Are Able to Eradicate Biofilm  
[PERSON], [PERSON], [PERSON], [PERSON], [PERSON], [PERSON], R. An Evaluation of the Usability of Argon Plasma-Treated Bacterial Cellulose as a Carrier for Controlled Releases of Biological Cellstrength and flexibility, thanks to which the dressing can adapt to various parts of the body. It should easily detach from the wound while changing the dressing, be biocompatible, and have homeostatic and antimicrobial action, preventing infection and bacterial biofilm formation [1; 3]. One of the biomaterials that meets most of the above criteria is bacterial cellulose (BC) [4]. In addition, the advantage of BC used in biomedicine is also the ability to modify its structure via various in situ and ex situ modifications [5]. The in situ modification of BC can be achieved by supplementing the culture medium with various types of organic and inorganic substances. These modifications can lead to enhanced porosity, water-holding capacity, and rehydration rates, which are crucial properties for wound-dressing materials. However, a significant challenge in the use of biomaterials involving BC is their susceptibility to bacterial infections, which restricts their applications in biomedicine. As a result, there has been a growing interest in the development of methods to confer antibacterial and antibiofilm properties to natural polymeric materials, including bacterial cellulose [6; 7; 8]. One of the possible methods for the ex situ modification of BC is the use of plasma, which is a well-known approach for effectively modifying the surfaces of various polymers. Depending on the composition of the gas phase, plasma technology offers the possibility of targeted polymer modification by introducing different functional groups or converting existing ones on the polymer surface. Various gas plasma technologies for polymer modification can be classified into two types based on the temperature of the gas: \"hot\" plasma and \"cold\" plasma [9]. Earlier studies have demonstrated that the surface of BC can be improved for various applications through the use of different types of plasmas.