# Applications of Pulsed Light Decontamination Technology in Food Processing: An Overview
## 5 Role of Pulsed Light Technology in Food Safety
\begin{table}
\begin{tabular}{c c c c c c} \hline
**Microorganism** & **Media** & \
\begin{tabular}{c} **Pulse Energy** \\ **(J/cm\({}^{3}\))** \\ \end{tabular} &
\begin{tabular}{c} **Treatment Time** \\ **(us) * \\ \end{tabular} & **Log\({}_{\text{St}}\) Reductions** & **Reference** \\ \hline _Alternaria alternate,_ & & & & & \\ _A. niger,_ & & & & & \\ _B. cincr,_ & & & & & & \\ _F. enzymegram,_ & & & & & & \\ _F. reaction,_ & & & & & & \\ _M. fractional expansus,_ & & & & & \\ _P. Lightening_ & & & & & \\ _Pitrophitschrisrophits and_ & & & & & \\ _Rizzus stolnorf_ & & & & & \\ _L. immocan_ & & Clear liquid broth & 12 & - & \textgreater{}6 & [61] \\ \hline _S. aureus, E. coli NCTC 5001,_ & & & & & & \\ _MRELian-instituted S. aureus_ & & & & & & \\ _MRELian LMC 15.975 and MRSA_ & & & & & & \\ _MRELian_ & & & & & \\ _MRELian_ & & & & & \\ _MRELian_ & & & & & \\ _S. enterica serovar Enteritidis_ & & Noble agar & 0.7 & - & 67 & [63] \\ \hline _E. coli ATCC 25.922 and E. coli_ & & Butterfield’s phosphate buffer & & 4 s & \textgreater{}8.5 & [64] \\ _O15/F.H7_ & & Typtic soy broth & 13.1 & 4 s (12 pulses/s) & 3.5 & [65] \\ \hline _Murine movements (MNV-1) and_ & & & & & \\ _I. subtilis A virus (HAV)_ & & & & 2 s & 5 & [65] \\ \hline _L. immocan_ NCTC 11,288 & & & & & 5.13 & \\ _E. coli_ & & Maximum recovery diluent & 28 & 8 s & 4.67 & [66] \\ K12 DSM 160 & & & & & \\ \hline _L. monocytogenes_ & & & & & \\ _B. subtilis_ & & Cell suspension & & & \\ strain ATCC 6633 & (OD\({}_{\text{300 nm}}\) - 0.8) & 0.6 & - & 8.7 & [50] \\ \hline _B. corras_ and & & & & & About 7 & [67] \\ \hline _L. monocytogenes_ & & & & & \\ \hline \end{tabular}
\end{table} 
Table 3: _Cont._ \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Microorganism** & **Media** & **Pulse Energy (/cm\({}^{2}\))** & **Treatment Time (sus)*** & **Logus Reductions** & **Reference** \\ \hline \multirow{3}{*}{_B. subtilis_} & Nutrient agar for vegetative cells & & & About 4 of spores & \\  & Typetic soy broth for spores & & - & \textgreater{}8 for vegetative & \\  & (cell density 10\({}^{6}\) cells/mL in both) & & & & cells & \\ \hline \multirow{3}{*}{_Geobacillus stearch/throughput/limits_} & Nutrient agar for both vegetative cells and & & & About 5.6 sor spores & \\  & spores & & - & About 1 for & \\  & & & & vegetative cells & \\ \hline \multirow{3}{*}{_P. aeruginosa_} & Plate count agar & 1.13 & 30 s & 3.63 & \\ \cline{3-5}  & & About 11 & 300 s & About 6.5 & \\ \cline{3-5}  & Solid tryptic soy agar Petri plates & - & 20 s & 5 & \\ \cline{3-5}  & 0.1\% peritone water solution in whirl pak & & & & [1] \\ \cline{3-5}  & 0.1\% peritone water solution in whirl pak & & & & \\ \cline{3-5}  & bags & - & 93 s 5 s & 1 & \\ \hline \multirow{3}{*}{_Bacillus subtilis spores_} & Clear liquid medium & 0.017 & - & 1 & [70] \\ \cline{3-5}  & & & - & \textgreater{}5.8 & \\ \cline{1-1} \cline{3-5}  & Phosphate buffered saline & 4.94 & - & \textgreater{}6.0 & \\ \hline \multirow{3}{*}{_E. coli_ K-12 MG1655} & & 76 /cm\({}^{2}\) & & & \\  & & wavelength of & - & 11 & \\ \cline{3-5}  & Lysogeny broth (LB) & 190 nm & & & \\ \cline{3-5}  & & 95 /cm\({}^{2}\) & & & \\ \cline{3-5}  & & wavelength of & & 6 & \\ \cline{3-5}  & & 240 nm & & & \\ \hline \hline \end{tabular}  
* Time mentioned as us unless specified.  
\end{table} 
Table 3: _Cont._