## 3 Results
### Exoproteomes Analysis During Semi-Solid Cultivation on Media Containing Various Types of Sawdust  
In total, 61 proteins (Figure 4, Supplementary Figure S1) were found on 2 DE maps of _C.__Lueve_ exoproteomes; 17-37% of them were proteins with unknown function, and the largest number was detected on GP-P (Figure 5). Of the total secretome protein pool, 35-49% were carbohydrate-active enzymes (CAZymes) [39]. At the same time, 50% of the total CAZyme pool on the GP medium were glycoside hydrolases (GHs); auxiliary activities (AAs) and carbohydrate esterases (CEs) classes were 37% and 13%, respectively. The ratio changed on the sawdust-containing media: GHs content decreased; AAs content, on the contrary, increased; CEs: 6-8% (GP-A and GP-P) and 22% (GP-B) (Figure 5). The _C.__Lueve_ GP exoproteome is represented by the largest repertoire of proteins (_n_ = 53). Approximately one-third (_n_ = 12) of these proteins were secreted on all the media, another third (_n_ = 12) were unique to medium, and the remaining proteins were secreted in different ways on the sawdust-containing media. The major proteins found on all the media were lacases (AA1_1), LPMOs X325 family (Supplementary Figure S2), GMC oxidoreductaseas (AA3) and FAD-binding domain-containing protein that was assigned to the AA7 CAZyme family (EC 1.1.3.-; oligosaccharides oxidase), as well as expansin-like proteins like fungal loosenins (Supplementary Figure S3) and lectins (Supplementary Figure S1, Figure 4). Their production was significantly increased on the sawdust-containing media. At the same time, the sawdust-containing media showed a decrease in the total number of proteins as compared to the GP medium (total 32, 28 and 22 proteins on GP-B, GP-A and GP-P media, respectively). A similar trend was also observed for other WRF (_Trametes hirsuta_; _Stecherinum ochracum_; _Peniophora lycii_) when cultivated under similar conditions [29; 30]. The GHs repertoire in _C.__Lueve_ exoproteomes presented GH16, GH17, GH18, GH31, GH37, GH47, GH51, GH72 CAZy families, among which only CAZy GH families 31 and 51 are involved in the biodegradation of plant cell walls. Among the CEs in the _C.__Lueve_ exoproteome, representatives of only two families (CE4 and CE16 CAZy) were found. TFK32449.1 and TFK42464.1 (proteins of the carboxylesterase/lipase family) were detected among the \"Others\" proteins in the _C.__Lueve_ secretomes.__Lue_ secretomes and lipase activities measured in culture liquids showed that esterase activity was higher than lipase activity on all the media, while both activities increased on the sawdust-containing media  
Figure 3: (**A**) White rot fungus (_Crucibulum__Lueve_ LE-BIN 1700) oxidase activity variation during stationary semi-solid cultivation on the control glucose–peptome (GP) medium and on the GP supplemented with birch (GP-B), alder (GP-A) and pine (GP-P) sawdust; (**B**) fungal biomass amount at the end of 30-day cultivation. Different letters indicate statistical differences between culture media at the same time point (_p_ < 0.05). in contrast to those on the GP medium (Table 1). The highest esterase and lipase activities were found on the GP-A and the GP-B media, respectively. Figure 4: Functional description and secretion conditions of proteins found in _C. leave_ LE-BIN 1700 exoproteomes during semi-solid cultivation on the control glucose-peptome (GP) medium and on the GP supplemented with birch (GP-B), alder (GP-A) and pine (GP-P) sawdust; aa—amino acid. The presence of proteins in the corresponding medium is indicated by a colored circle. \begin{table} \begin{tabular}{c c c} \hline \hline
**Culture Liquid** & **Esterase Activity, \(\times\)10\({}^{-2}\) U/mL** & **Lipase Activity, \(\times\)10\({}^{-2}\) U/mL** \\ \hline GP & \(1.0\pm 0.1\) & 0.4 \(\pm\) 0.1 \\ GP-B & \(1.6\pm 0.1\) & 0.8 \(\pm\) 0.1 \\ GP-A & \(2.4\pm 0.2\) & 0.5 \(\pm\) 0.1 \\ GP-P & \(2.0\pm 0.1\) & 0.4 \(\pm\) 0.1 \\ \hline \hline \end{tabular}