## 2 Results and Discussion  
### Profiling of the Phenolic Composition  
The (poly)phenolic profile of the proprietary rosemary extract rich in carnosic acid was evaluated using an UHPLC-ESI-MS\({}^{n}\) untargeted method consisting of two complementary mass spectrometry (MS) conditions [21]. About 190 mass spectra were assessed for each analytical replicate and MS operating condition in this comprehensive approach for a complete screening of (poly)phenolic compounds. This procedure allowed a detailed evaluation of the rosemary extract phenolic fraction and the tentative identification of up to 57 phytochemicals (Table 1). The most represented classes of (poly)phenolic compounds in the extract were diterpenoids and flavonoids (flavones, flavanones, and flavonoids), with a total of 24 molecules identified for each class. Some phenolic acids and lignans, as well as one triterpen, were also identified. \begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline ID. & **Compounds** & **RT** & \begin{tabular}{c} **[M \(-\) H]\({}^{-}\)** \\ **(m/z)** \\ \end{tabular} & \
\begin{tabular}{c} **MS\({}^{2}\) ion** \\ **Fragments** \\ **(m/z)** \\ \end{tabular} &
\begin{tabular}{c} **MS\({}^{3}\) ion** \\ **Fragments** \\ **(m/z)** \end{tabular} & **Exp. 1 \({}^{\circ}\)** & **Exp. 2 \({}^{\circ}\)** & **Ident. \({}^{\circ}\)** \\ \hline
**1** & Caffe acid & 6.82 & **179** & 135 & & x & x & Std \\
**2** & Medioresinol & 7.18 & **387** & **207\({}^{\circ}\)**, 163, & & 163 & x & x & [4; 12] \\
**3** & \(p\)-Coumaric acid & 7.93 & **163** & **119** & 119 & x & x & [4] \\
**4** & Luteolin-rutinoside & 8.78 & **593** & **285** & 285, 241, 175, & x & - & [4; 22] \\
**5** & Luteolin-hexoside & 8.98 & **447** & **285**, 378 & 285, 241, 267, & x & x & [4] \\  & & & & & & 199, 175 & & \\
**6** & Isondhametin-3-O-hexoside & 9.28 & **477** & 315, 300, 357, & & 300 & x & x & [4] \\
**7** & 4-hydroxybenzoic acid & 9.47 & **137** & 93, 137 & & & x & [4] \\
**8** & Apigenin-7-O-glucose & 9.82 & **431** & **269** & 183, 281 & x & x & [23] \\  & & & & & & 286, 242, 257, & & x & Std \\
**9** & Hespertien & 9.87 & **609** & **301** & 283, 125 & & & \\
**10** & Homoplantaginin (Hispidulin & 10.04 & **461** & **299, 446, 284, & & 284, & 258, 179 & x & x & [3] \\
**11** & Rosmartensic acid & 10.11 & **359** & **161, 179, 197, & & & & & \\  & & & & & & 223 & 161, 133 & x & x & Std \\ \hline \hline \end{tabular}
\end{table} 
Table 1: (Poly)phenolic compounds in rosemary extract identified by ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS\({}^{n}\)) in negative ionization mode under different mass spectrometry (MS) conditions. _Molecules_**2016**, _21_, 1576  
\begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline
**ID. ** & **Compounds** & **RT** & **[**\(\mathbf{M-H}\)**]\({}^{-}\) & **MS\({}^{2}\) ion** & **MS\({}^{3}\) ion** & & & & \\  & & **(min)** & **(m/z)** & **Fragments** & **Fragments** & **Exp. 1 \({}^{\circ}\)** & **Exp. 2 \({}^{\circ}\)** & **Ident. \({}^{\ddagger}\)** \\ \hline
**12** & Luteolin-7-O-glucuronwide & 10.28 & **461** & **285** & \begin{tabular}{c} 241, 217, 175, \\ 199 \\ \end{tabular}  & x & x & [4] \\
**13** & \begin{tabular}{c} Dihydroxy-dimethoxyosyltransferase \\ derivative \\ \end{tabular}  & 10.33 & **387** & **313**, 343 & 298 & x & - & [4,24] \\
**14** & \begin{tabular}{c} Dihydroxy-dimethoxyosyltransferase \\ \end{tabular}  & 10.71 & **313** & **298** & 269, 283, 297, & x & - & [4,24] \\
**15** & \ \begin{tabular}{c} Medioresinol derivative \\ \end{tabular}  & 11.22 & **593** & \begin{tabular}{c} **387**, 561, 519, 207, 163, 369 \\ \end{tabular}  & x & x & [12] \\
**16** & \begin{tabular}{c} Dihydroxy-dimethoxyosyltransferase \\ \end{tabular}  & 11.24 & **313** & **298** & 150 & x & - & [4,24] \\
**17** & Luteolin-3-acetyl-O-glucuron & 11.25 & **503** & **285**, 399, 443 & 241, 243, 217, & x & x & [13,23] \\
**18** & \begin{tabular}{c} Medioresinol-glucuron \\ \end{tabular}  & 11.37 & **563** & **387**, 531, 489 & 207, 163, 369 & x & x & [12] \\
**19** &