# Seasonal Variability of Volatilome from _Dictyota dichotoma_
## 2 Results and Discussion
### Variations of Validime of Air-Dried D. dichotomous Obtained by Hydrodistillation  
As expected, the VOCs chemical profile of HD-DrDd was remarkably different from HS-DrDd and it showed similarity to HD-FrDd (Table 3). The major compounds were similar for HD-FrDd and prenylated-guaiane diterpenes were present: isopachyidcyl0 A (up to 25.03%) and pachyidcyl0 A (up to 5.12%). As in HD-FrDd, abundant diterpene alcohol was cembra-4,7,11,15-tetraen-3-ol up to 26.90% followed by cembrene A and cembrene. \begin{table}
\begin{tabular}{c c c c c c c c c c c c} \hline \hline  & & & \multicolumn{3}{c}{**May**} & \multicolumn{3}{c}{**June**} & \multicolumn{3}{c}{**July**} & \multicolumn{3}{c}{**August**} & \multicolumn{3}{c}{**September**} \\ \cline{3-13}
**No. ** & **Compound** & **RI** & **Fr** & **Dr** & **Fr** & **Dr** & **Fr** & **Dr** & **Fr** & **Dr** & **Fr** & **Dr** \\ \cline{3-13}  & & & & & & & & & & & & & \\  & & & & & & & & & & & & & \\  & & & & & & & & & & & & & \\
71 & Cubenol & 1647 & 0.31 & 0.19 & 0.41 & 0.26 & 0.07 & 0.10 & 0.50 & 0.13 & 0.16 & 0.21 \\
72 & \(\alpha\)-Cadinol & 1660 & 0.32 & 0.14 & 0.38 & 0.22 & 0.06 & 0.12 & 0.53 & 0.14 & 0.20 & 0.21 \\
73 & (_E_)-Heptadec-8-ene & 1683 & - & 0.16 & - & 0.15 & - & - & - & - & - & - \\
74 & (_Z_,_E_)-Farnesyl & 1688 & 1.40 & 0.99 & 1.20 & 1.23 & 0.16 & 0.22 & - & 0.29 & - & 0.35 \\
75 & (_E_)-Heptadec-8-ene & 1696 & 0.68 & 0.33 & 0.69 & 0.56 & 0.08 & - & 0.23 & 0.09 & - & - \\
76 & Heptadecane \({}^{\text{a}}\) & 1700 & 3.60 & 0.97 & 3.63 & 1.28 & 1.30 & 0.11 & 4.61 & 0.51 & 1.72 & 1.32 \\
77 & Pentadecan \({}^{\text{a}}\) & 1718 & 1.49 & 0.98 & 1.38 & 0.84 & 0.42 & 0.07 & 1.26 & 0.16 & 0.49 & 0.60 \\
78 & Tetadecan acid \({}^{\text{a}}\) & 1770 & 0.19 & 0.84 & 0.42 & 1.75 & 0.04 & 4.94 & - & 1.296 & 0.26 & 3.93 \\
79 & (_Z_,_E_)-Farnesyl acetate & 1799 & 0.78 & 0.55 & 0.73 & 0.78 & 0.27 & 0.35 & 1.29 & 0.85 & 0.25 & 0.84 \\
80 & 6,10,14-Trimientlypendadecan-2-one \({}^{\text{a}}\) & 1850 & - & 0.29 & - & 0.18 & - & - & - & - & - & 0.11 \\
81 & Nonadec-1-ene & 1897 & - & 0.40 & - & 0.39 & - & - & - & 0.11 & - & 0.16 \\
82 & Cembrene & 1930 & 0.08 & 0.27 & 0.12 & 0.28 & 0.09 & 0.07 & - & 0.09 & - & 0.18 \\
83 & Palmitic acid \({}^{\text{a}}\) & 1962 & - & - & - & - & - & - & - & 1.23 & - \\
84 & Cembrene A & 1969 & 0.42 & 0.81 & 0.59 & 1.07 & 0.44 & 0.85 & 0.61 & 1.17 & 0.35 & 1.00 \\
85 & Hexadecanoic acid \({}^{\text{a}}\) & 1975 & - & 1.07 & - & 1.32 & - & 0.26 & - & 0.54 & - & - \\
86 & Ethly hexadecanone \({}^{\text{a}}\) & 1987 & - & 0.49 & - & 0.61 & - & 0.43 & - & 1.12 & - & 0.54 \\
87 & (_Z_)-Octadecen-9-al & 1997 & - & 3.93 & - & 4.69 & - & 2.25 & - & 1.66 & - & 3.15 \\
88 & Ocadecan \({}^{\text{a}}\) & 2025 & - & - & - & 0.32 & - & - & - & - & - & - \\
89 & Methyl octadecyl ether & 2036 & - & 3.33 & - & 5.09 & - & 4.33 & - & 3.44 & - & 3.68 \\
90 & Methyl (all _Z_) eicosa-5,8,11,14, & & 2040 & 0.34 & 0.21 & - & - & - & - & 0.22 & - & 0.25 & - \\
17-pentenatoate & & & & & & & & & & & & & \\
91 & ciosa-5,8,11,4-tetraen & 2045 & 8.97 & 8.20 & 6.51 & 7.49 & 9.73 & 6.95 & 11.01 & 7.28 & 10.31 & 8.70 \\
92 & (_Z_,\(Z\),_Z_)-Octadec-9,2,12,15-trien-1-ol & 2055 & 1.08 & 0.25 & 1.10 & - & - & - & - & - & - \\
93 & (_Z_)-Octadec-9-en-1-ol & 2061 & 1.58 & 0.66 & 1.55 & 0.66 & 4.44 & - & 2.43 & - & 1.81 & - \\
94 & (_E_)-Phytl & 2116 & 1.05 & - & - & - & - & - & - & - & - & - \\
95 & Pachydicyl0 A & 2120 & 1.86 & 2.25 & 5.19 & 2.60 & 5.54 & 5.12 & 2.09 & 20.4 & 2.93 & 3.67 \\
96 & Isopachydictyl0 A & 2128 & 19.72 & 25.03 & 15.01 & 18.79 & 18.88 & 16.02 & 15.77 & 17.92 & 16.91 & 19.15 \\
97 & (_Z_)-Octadec-9-enic acid & 2147 & 1.90 & 6.61 & 2.85 & 6.28 & 6.15 & 10.81 & 2.71 & 9.08 & 3.73 & 11.05 \\
98 & (_E_)-Geranygeraniol & 2184 & 0.34 & 0.50 & 0.44 & 0.58 &The most striking difference between HD-FrDd and HD-DrDd was the increase (in all months) of (\(Z\))-octadec-9-enoic acid up to 11.05% and tetradecanoic acid up to 12.96%. (\(Z\))-Octadecen-9-al appeared up to 4.69% in HD-DrDd. All these compounds indicate oxidation processes during drying.