# Hepatoprotective Effect of Alpinetin on Thioacetamide-Induced Liver Fibrosis in Sprague Dawley Rat
## 3 Results
### Influence of Alpinetin on Biochemical Parameters  
TAA-induced impairment in hepatic tissues and the grade of injury were measured by substantial serum amounts of specific hepatic enzymes, for instance, ALP, ALT, AST, and bilirubin. Liver enzymes appeared provocatively raised in the TAA control group owing to show liver enzymes as a consequence of tissue damage. Rats fed orally with Silymarin or Alpinetin exhibited significantly lesser enzyme activity and bilirubin (Table 7).  
\begin{table}
\begin{tabular}{c c c c} \hline \hline
**Groups** & **SOD** & **CAT** & **MDA** \\  & **U/mg Protein** & **U/mg Protein** & **U/mg Protein** \\ \hline Normal Control & 15.87 \(\pm\) 0.60 b  & 36.84 \(\pm\) 0.8 b  & 1.18 \(\pm\) 0.01 b  \\ TAA Control & 8.35 \(\pm\) 0.24 a  & 16.95 \(\pm\) 0.52 a  & 4.62 \(\pm\) 0.13 a  \\ Silymarin (50 mg/kg) +TAA & 12.21 \(\pm\) 0.26 b  & 28.07 \(\pm\) 0.57 b  & 1.54 \(\pm\) 0.09 b  \\ Alpinetin (30 mg/kg) + TAA & 13.92 \(\pm\) 0.05 b  & 32.78 \(\pm\) 0.90 b  & 2.16 \(\pm\) 0.02 b  \\ Alpinetin (60 mg/kg) + TAA & 13.09 \(\pm\) 0.30 b  & 30.81 \(\pm\) 0.78 b  & 1.87 \(\pm\) 0.03 b  \\ \hline \hline \end{tabular}  
* The value was represented by mean \(\pm\) S.E.M (\(n=6\)). According to [PERSON]’s post hoc test at a \(p<0.05\) significant level, the values of different superscripts within the same column are significantly different. Superscript a and b are used for describing the difference between groups, if (a and b) present in the same column it means a significant difference between groups.  
\end{table}
Table 6: Results of Alpinetin on SOD, CAT, and MDA content in liver homogenate in the TAA-induced liver fibrosis in rats.  
\begin{table}
\begin{tabular}{c c c c} \hline \hline
**Groups** & **Body Weight** & **Liver Weight** & **Liver Index (\%)** \\  & **(gm)** & **(gm)** & **LW/BW\%** \\ \hline Normal Control & 330 \(\pm\) 3.72 b  & 10.15 \(\pm\) 0.08 b  & 3.078 \(\pm\) 0.01 b  \\ TAA Control & 168 \(\pm\) 1.59 a  & 12.87 \(\pm\) 0.03 a  & 7.66 \(\pm\) 0.06 a  \\ Silymarin (50 mg/kg) + TAA & 306.16 \(\pm\) 4.17 b  & 10.32 \(\pm\) 0.06 b  & 3.37 \(\pm\) 0.06 b  \\ Alpinetin (30 mg/kg) + TAA & 231 \(\pm\) 1.88 b  & 10.45 \(\pm\) 0.04 b  & 4.52 \(\pm\) 0.02 b  \\ Alpinetin (60 mg/kg) + TAA & 247.66 \(\pm\) 1.54 b  & 10.22 \(\pm\) 0.03 b  & 4.127 \(\pm\) 0.03 b  \\ \hline \hline \end{tabular}  
* The value was represented by mean \(\pm\) S.E.M (\(n=6\)). According to [PERSON]’s post hoc test at a \(p<0.05\) significant level, the values of different superscripts within the same column are significantly different. Superscript a and b are used for describing the difference between groups, if (a and b) present in the same column it means a significant difference between groups.  
\end{table}
Table 5: Influence of Alpinetin on body mass and liver heaviness in TAA-induced liver cirrhosis, experimental rats.  
The protein, besides albumin values, perceptively substandard hepatotoxic group associated rats fed with Silymarin or Alpinetin groups, representing acute hepatocellular injury. Silymarin or Alpinetin-fed rats reinstated the protein and albumin closer to their normal ranges. Therefore, Silymarin or Alpinetin responded to the toxic consequence of TAA by returning to standard liver functions (Table 7).