## 4 Discussion
Studies of absorption, distribution, metabolism, and excretion (ADME) help us to predict the drug's efficacy [72]. The results of this computational analysis show that americine, an alkaloid, has the potential to interact with all four target proteins in the same way that their known inhibitors/drugs do. When the MolDock score is negative, it indicates that the ligand binds to its targets without being forced. A higher negative score implies stronger chemical bonding. Americine shows a docking score of \(-112.82\) in EGFR, while positive controlgefitinib shows \(-103.14\). Similarly, for K-Ras, americine shows \(-139.02\) and positive control adagrasib shows \(-136.77\). In the case of PI3K, docking scores of \(-115.76\) and \(-113.52\) are shown by americine and positive control duvelisib, respectively. Lastly, \(-144.45\) was scored by americine against a score of \(-138.89\) of positive control dabrafenib for the target B-raf. Mutations in K-Ras and PI3K make normal cells resistant to death via apoptosis [73; 74], while mutations in EGFR and B-Raf cause unchecked cell proliferation [75; 76]. Americine prevents cell growth and induces apoptosis to treat NSCLC as it inhibits all four of these targets. Similar studies by [PERSON] et al. [65] were based on molecular docking, with Oat Avenanthramides as EGFR inhibitors, which resulted in the inhibition of growth and migration in NSCLC cells. Another molecular docking analysis of B-Raf with linalool by [PERSON] et al. [77] revealed the chemopretive activity against lung cancer. Through molecular docking, we know that the plant secondary metabolite americine has the potential to bind and inhibit the drug targets of NSCLC; however, this does not guarantee that it will be an effective drug. We have completed the ADMET for predicting the compound's activity to foretell its potential as a pharmaceutical candidate. These in silico toxicological analysis techniques are generally accepted by the pharmaceutical industry for use in decision-making throughout the medication development process for validation [72; 78; 79] and have been shown to drastically cut down on the number of chemicals tested on animals [80]. Adverse drug reactions, metabolic effects, and drug transport predicted that americine would be well absorbed by the human gastrointestinal tract [80]. BOILED-Egg analysis confirms the low risk of affecting regular CNS activity, demonstrating the compound's inactivity. Similar studies can be found in the work of [PERSON] et al. [72]. Some additional pharmacokinetic and drug-likeness profiling was performed with the help of the SwissADME server [72]. With only one infraction of the drug-likeness rule and a moderate Abbot bioavailability score, americine stands out as the most promising candidate for use as a structural template for the development of new drugs. This research foretold that the anti-lung cancer benefits of the americine compounds would be exerted via a combination of mechanisms. With current therapies having strong side-effects and being too expensive to reach the hand of the common person, further research must be conducted with this potent phytocompound against such deadly diseases. Plant-based therapeutics offer a feasible replacement for conventional cancer treatments such as chemotherapy and radiation therapy, with the added benefits of reduced side-effects and lower treatment costs.