## 3 Results
To account for the time factor, subsequent experiments with serial dilution and vigorous shaking of samples took exactly 90 min (from the beginning of sample preparation to measurement). It was shown that 90 min after the end of the exposure of water to the CMF, the extent of the effect decreased only slightly (by about 5%) relative to the same value after 30 min. After confirming the ability of the CMF to induce in water new biophysical properties, i.e., to influence ROS production by neutrophils, we investigated the dependencies of the preservation of this activity within a range of serial dilutions and when the static component of the combined magnetic field was changed. C12, C30, and C50 dilutions were selected from the set of 50 centesimal serial dilutions, since these dilution levels showed a significant effect in previous studies [11; 16]. The parameters of the SMF component in the CMF varied from the minimum possible value (about 10 nT) to 20,000 nT (20 \(\upmu\)T) (Figure 3). Figure 3: _Cont._  
As seen in Figure 3, all tested dilutions ('C12', 'C30', and 'C50'), which were prepared in the GMF, exert a significant effect on the chemiluminescence intensity (ROS production  
Figure 3: Testing the properties of water after treatment with the CMF (alternating field 12.6 Hz, 50 nT, static field 60 \(\mu\)T), followed by serial dilution and intense shaking at different values of the residual SMF All samples were tested after dilution: panel **(a)** C12—(100\({}^{12}\) times dilution); panel **(b)** C30—(100\({}^{30}\) times dilution); panel **(c)** C50—(100\({}^{50}\) times dilution). The measurements of normalized chemiluminescence are presented as Mean \(\pm\) Standard deviation. Normalized chemiluminescencee was calculated by dividing the maximum chemiluminescencee value measured for a particular sample by the maximum value obtained for control water. The value of chemiluminescencee for “Water” sample (Control) was taken as 100% (not shown on the graph). * \(p\) \(<\) 0.05 (calculated values for each comparison presented on section **(e)**. **(d,e)**–_p_-values obtained during statistical comparison of the results shown in **(a–c)** using the Student/Welch _l_-test and Mann–Whitney test. A comparison was made between the chemiluminescencee intensity indicated for: **(d)**—C12, C30, C50 dilutions (designations are provided at the top of each column) and control water taken as 100%; _p_-values marked in red—no differences from control water (negative control), yellow—borderline values, green—there are differences from control water. **(e)**—C12, C30, C50 dilutions (designations are provided at the top of each column) made at the SMF (has different values) and the identical sample, but prepared at the GMF; _p_-values marked in red—there are differences from a similar sample prepared at the GMF (positive control), green—no differences from a similar sample prepared at the GMF. by neutrophils). The differences from the control samples (intact water) range from 30 to 60% (\(p<0.05\)) (gray bars in Figure 3a-c and statistics in Figure 3d for GMF samples). At the same time, the group of samples prepared at a near-zero magnetic field (\(\sim\)10 nT) does not have such activity; all measurement results do not differ from the controls (hatched bars in Figure 3a-c and statistics in Figure 3d for SMF samples). Next, when examining similar samples with a gradual increase in the field induction (from 10 nT to 20,000 nT), at which samples were prepared, the \(p\)-values decreased (Figure 3d), and the differences in normalized chemiluminescence varied from about a 5-10% increase at 10 nT (\(p>0.05\)) to a 42-56% increase at 20,000 nT (\(p<0.05\)). Importantly, C12, C30, and C50 dilutions of water prepared under different magnetic fields (at GMF or SMF of various induction) exert different effects on the chemiluminescence intensity of neutrophils.