# Microbiota and Metabolite Profiling of Spoiled Spanish-Style Green Table Olives
## Results and Discussion
### Metabolite Composition in Brine Samples
\begin{table}
\begin{tabular}{l c c c c c c c c c c c} \hline \hline \multicolumn{1}{c}{**Compounds**} & \multicolumn{1}{c}{**Samples \({}^{\bullet}\)**} & \multicolumn{1}{c}{**} & \multicolumn{1}{c}{**} & \multicolumn{1}{c}{**} & \multicolumn{1}{c}{**} & \multicolumn{1}{c}{**} & \multicolumn{1}{c}{**} & \multicolumn{1}{c}{**} & \multicolumn{1}{c}{**} & \multicolumn{1}{c}{**} & \multicolumn{1}{c}{**} & \multicolumn{1}{c}{**} \\ \cline{2-13} \multicolumn{1}{c}{**Non-volatile acids \({}^{\bullet}\)**} & & & & & & & & & & & & & \\ \multicolumn{1}{c}{Lactic acid} & \(5.20\pm 0.20\) & \(3.70\pm 0.08\) & ND & ND & ND & ND & ND & ND & ND & ND \\ \multicolumn{1}{c}{Succinic acid} & \(0.24\pm 0.09\) & \(0.16\pm 0.01\) & \(0.33\pm 0.03\) & \(0.27\pm 0.01\) & \(0.27\pm 0.01\) & \(0.22\pm 0.01\) & \(0.22\pm 0.01\) & \(0.23\pm 0.00\) & \(0.12\pm 0.00\) & \(0.12\pm 0.00\) \\ \hline \multicolumn{1}{c}{**Valatile acids \({}^{\bullet}\)**} & & & & & & & & & & \\ \multicolumn{1}{c}{Acctic acid} & \(891\pm 32\) & \(124\pm 38\) & \(4598\pm 80\) & \(3528\pm 182\) & \(1392\pm 164\) & \(2322\pm 511\) & \(2161\pm 415\) & \(2446\pm 19\) & \(1782\pm 724\) & \(3107\pm 536\) \\ \multicolumn{1}{c}{Propionic acid} & \(107\pm 22\) & \(1854\pm 125\) & \(5378\pm 80\) & \(5284\pm 200\) & \(3022\pm 98\) & \(3727\pm 391\) & \(4216\pm 109\) & \(4302\pm 192\) & \(2846\pm 127\) & \(3075\pm 115\) \\ \multicolumn{1}{c}{Isobutyrate acid} & \(1.6\pm 0.3\) & \(31\pm 0.1\) & ND & \(5\pm 2\) & \(33\pm 0.3\) & ND & \(35\pm 0.0\) & \(32\pm 0.0\) & ND & \(19\pm 0.8\) \\ \multicolumn{1}{c}{Burylric acid} & \(66\pm 0.5\) & \(12\pm 4\) & \(603\pm 80\) & \(440\pm 12\) & \(630\pm 49\) & \(538\pm 16\) & \(185\pm 22\) & \(135\pm 1\) & \(164\pm 10\) & \(313\pm 3\) \\ \multicolumn{1}{c}{Valeric acid} & \(0.9\pm 0.5\) & \(1\pm 1\) & \(1068\pm 127\) & \(792\pm 73\) & \(1560\pm 26\) & \(1001\pm 62\) & \(386\pm 39\) & \(256\pm 2\) & \(350\pm 7\) & \(513\pm 50\) \\ \multicolumn{1}{c}{Capactic acid} & \(0.2\pm 0.0\) & \(0.2\pm 0.1\) & \(65\pm 7\) & \(38\pm 5\) & \(194\pm 6\) & \(59\pm 1\) & \(10\pm 1\) & \(6\pm 2\) & \(11\pm 0\) & \(42\pm 2\) & \(2\pm 2\) \\ \multicolumn{1}{c}{Heptanoic acid} & ND & ND & \(2.2\pm 0.4\) & \(1.3\pm 0.3\) & \(111.1\pm 0.1\) & \(11.0\pm 0.2\) & \(0.2\pm 0.0\) & \(0.1\pm 0.0\) & \(0.2\pm 0.0\) & \(0.3\pm 0.0\) \\ \multicolumn{1}{c}{Cydebnoxazole acid} & \(1.0\pm 0.5\) & \(0.6\pm 0.5\) & \(422\pm 16\) & \(31\pm 0\) & \(61\pm 9\) & \(0.6\pm 0.8\) & \(841\pm 100\) & \(729\pm 31\) & \(401\pm 1\) & \(479\pm 56\) \\ \hline \multicolumn{1}{c}{**Alcoholics \({}^{\bullet}\)**} & & & & & & & & & & \\ \multicolumn{1}{c}{Ethanol \({}^{\bullet}\)} & \(0.12\pm 0.02\) & \(0.14\pm 0.03\) & \(0.44\pm 0.02\) & \(0.27\pm 0.07\) & \(0.15\pm 0.01\) & \(0.23\pm 0.05\) & \(0.28\pm 0.02\) & \(0.27\pm 0.03\) & \(0.46\pm 0.06\) & \(0.45\pm 0.01\) \\ \multicolumn{1}{c}{2-butanol} & \(8\pm 1\) & \(6.5\pm 0.2\) & \(14\pm 2\) & \(102\pm 0.1\) & \(14\pm 3\) & \(264\pm 0.3\) & \(55\pm 5\) & \(16.8\pm 0.8\) & \(26\pm 4\) & \(36\pm 5\) & \(36\pm 5\) \\ \multicolumn{1}{c}{1-Propanol \({}^{\bullet}\)} & \(46\pm 2\) & \(558\pm 4\) & \(42\pm 2\) & \(42\pm 2\) & \(31\pm 6\) & \(45\pm 1\) & \(43\pm 3\) & \(3\pm 9\) & \(12\pm 36\) & \(36\pm 5\) & \(295\pm 0.5\) \\ \multicolumn{1}{c}{1-butanol} & \(1\pm 2\) & \(244\pm 0.01\) & \(33\pm 0.06\) & \(53\pm 0.2\) & \(96\pm 2\) & \(4.30\pm 0.05\) & \(2\pm 1\) & \(0.58\pm 0.04\) & \(4\pm 1\) & \(1\pm 0.07\) \\ \multicolumn{1}{c}{1-butanol} & ND & ND & \(7\pm 9\) & \(0.5\pm 0.5\) & \(33\pm 8\) & \(8.69\pm 0.08\) & \(6\pm 3\) & \(2.15\pm 0.09\) & \(24\pm 0.4\) & \(2.99\pm 0.06\) \\ \multicolumn{1}{c}{3-Methyl-1-pentanol} & \(0.3\pm 0.4\) & \(0.1\pm 0.1\) & \(0.62\pm 0.02\) & \(0.52\pm 0.07\) & \(0.37\pm 0.05\) & \(0.38\pm 0.01\) & \(0.2\pm 0.2\) & \(0.29\pm 0.08\) & \(0.32\pm 0.07\) & \(0.13\pm 0.06\) \\ \multicolumn{1}{c}{1-Hexanol \({}^{\bullet}\)} & \(0.90\pm 0.09\) & \(0.19\pm 0.03\) & \(1.42\pm 0.03\) & \(18.5\pm 0.04\) & \(51\pm 0\) & \(1.40\pm 0.01\) & \(12\pm 0.6\) & \(0.5\pm 0.2\) & \(0.92\pm 0.09\) & \(0.80\pm 0.03\) \\ \multicolumn{1}{c}{2-3-Hexanol \({}^{\circ}\)} & \(3.00\pm 0.02\) & \(27.0\pm 0.3\) & \(3.8\pm 0.3\) & \(3.6\pm 0.2\) & \(21\pm 0.3\) & \(27\pm 0.1\) & \(4.2\pm 3.2\) & \(3.5\pm 0.2\) & \(3.7\pm 0.1\) & \(2.6\pm 0.1\) \\ \multicolumn{1}{c}{2-Ethyl-1-hexanol} & \(5.8\pm 0.2\) & \(5.9\pm 0
\begin{table}
\begin{tabular}{l c c c c c c c c c c} \hline \hline \multicolumn{1}{c}{**Compounds**} & \multicolumn{6}{c}{**Samples \({}^{\star}\)**} \\ \cline{2-10} \multicolumn{1}{c}{**Compounds**} & \multicolumn{1}{c}{**G1 (control)**} & \multicolumn{1}{c}{**G2 (control)**} & \multicolumn{1}{c}{**GFC1**} & \multicolumn{1}{c}{**GFC2**} & \multicolumn{1}{c}{**GF11**} & \multicolumn{1}{c}{**GFL2**} & \multicolumn{1}{c}{**GFC1**} & \multicolumn{1}{c}{**GCC2**} & \multicolumn{1}{c}{**GCC1**} & \multicolumn{1}{c}{**GCC2**} & \multicolumn{1}{c}{**GCC11**} & \multicolumn{1}{c}{**GCC12**} \\ \hline
**Edges \({}^{\star}\)** & & & & & & & & & & & \\ \multicolumn{1}{c}{Methyl preanoate} & \(28\pm 2\) & \(28.26\pm 0.01\) & \(\mathcal{O}7\pm 0.3\) & \(33.6\pm 0.7\) & \(32\pm 7\) & \(19\pm 5\) & \(22\pm 4\) & \(43\pm 0.9\) & \(26\pm 5\) & \(159\pm 0.6\) \\ \multicolumn{1}{c}{Propyl acetate} & \(24\pm 3\) & \(19.9\pm 0.6\) & \(20\pm 1\) & \(12.35\pm 0.08\) & \(5\pm 1\) & \(144\pm 0.2\) & \(15\pm 1\) & \(83\pm 0.4\) & \(15\pm 2\) & \(97\pm 0.2\) \\ \multicolumn{1}{c}{Methyl 2-methylbutanoate} & \(32\pm 0.4\) & \(315\pm 0.03\) & \(25\pm 0.1\) & \(32\pm 0.2\) & \(36\pm 0.3\) & \(30\pm 0.1\) & \(11\pm 0.1\) & \(2.2\pm 0.1\) & \(2.5\pm 0.3\) & \(151\pm 0.7\) \\ \multicolumn{1}{c}{Ethyl abundance} & ND & ND & \(10.27\pm 0.03\) & ND & ND & ND & ND & ND & ND & \(152\pm 0.2\) \\ \multicolumn{1}{c}{Propyl propionate} & \(82\pm 5\) & \(88\pm 1\) & \(110\pm 4\) & \(\mathcal{G}7.20\pm 0.01\) & \(28\pm 2\) & \(634\pm 0.6\) & \(44\pm 5\) & \(58\pm 1\) & \(68\pm 6\) & \(628\pm 0.8\) \\ \multicolumn{1}{c}{Methyl pentaanoate} & \(0.75\pm 0.00\) & \(0.53\pm 0.03\) & \(25\pm 5\) & \(21\pm 6\) & \(66\pm 5\) & \(31\pm 3\) & \(23\pm 3\) & \(360\pm 0.2\) & \(58\pm 2\) & \(70\pm 2\) \\ \multicolumn{1}{c}{Hybrid pentaanoate} & \(0.4\pm 0.6\) & \(0.64\pm 0.0\) & ND & ND & ND & ND & \(10\pm 5\) & \(98\pm 0.2\) & \(37\pm 1\) & \(81\pm 6\) \\ \multicolumn{1}{c}{Methyl hexanoate} & ND & ND & \(83\pm 0.7\) & \(9\pm 2\) & \(88\pm 6\) & \(19\pm 2\) & \(6\pm 2\) & \(7.7\pm 0.1\) & \(19\pm 2\) & \(566\pm 0.4\) \\ \multicolumn{1}{c}{Propyl pentanoate} & \(0.3\pm 0.4\) & \(0.58\pm 0.04\) & \(74\pm 18\) & \(18\pm 3\) & \(90\pm 2\) & \(849\pm 0.9\) & \(48\pm 0.9\) & \(48\pm 16\) & \(26\pm 1\pm 0.4\) & \(810\pm 21\) \\ \multicolumn{1}{c}{Ethyl Isaaconic} & ND & ND & ND & ND & ND & ND & ND & ND & ND & \(45\pm 0.8\) \\ \multicolumn{1}{c}{Methyl heptanoate} & ND & ND & ND & ND & \(24\pm 0.3\) & \(0.24\pm 0.2\) & ND & ND & ND & ND & ND \\ \multicolumn{1}{c}{Propyl hexanoate} & ND & ND & \(194\pm 0.09\) & \(0.2\pm 0.3\) & \(0.39\pm 0.99\) & \(13\pm 0.1\) & \(0.7\pm 0.4\) & \(0.42\pm 0.06\) & \(21\pm 0.5\) & \(100\pm 0.2\) \\ \multicolumn{1}{c}{Methyl cycloheancarboxylic} & \(0.43\pm 0.02\) & \(0.39\pm 0.02\) & \(13\pm 2\) & \(0.9\pm 0.2\) & \(23\pm 0.1\) & \(0.04\pm 0.01\) & \(54\pm 3\) & \(39\pm 0.4\) & \(48\pm 2\) & \(457\pm 0.7\) \\ \multicolumn{1}{c}{Hybrid cyclohexanecarboxylic} & \(0.34\pm 0.01\) & \(0.27\pm 0.01\) & \(11.1\pm 0.01\) & ND & ND & ND & \(26\pm 9\) & \(31.6\pm 0.4\) & \(33\pm 1\) & \(47\pm 4\) \\ \multicolumn{1}{c}{Bemy1 pyropanec} & ND & \(0.82\pm 0.00\) & \(0.40\pm 0.03\) & \(0.36\pm 0.05\) & \(0.43\pm 0.01\) & \(19\pm 0.4\) & \(18\pm 0.1\) & \(0.98\pm 0.09\) & \(0.97\pm 0.04\) \\ \multicolumn{1}{c}{2-methylheptyl acetate} & \(1.23\pm 0.03\) & \(12\pm 0.04\) & \(19.7\pm 0.00\) & \(1.6\pm 0.2\) & \(0.5\pm 0.3\) & \(0.96\pm 0.08\) & \(6\pm 2\) & \(4.6\pm 0.3\) & \(3.53\pm 0.02\) & \(1.22\pm 0.02\) \\ \multicolumn{1}{c}{Methyl hyd hydcominate} & ND & ND & \(0.10\pm 0.04\) & \(0.05\pm 0.01\) & \(0.12\pm 0.03\) & \(0.99\pm 0.01\) & \(1.2\pm 0.5\) & \(1.09\pm 0.06\) & \(1.32\pm 0.08\) & \(1.54\pm 0.09\) \\ \multicolumn{1}{c}{Buzzy pentanoate} & ND & \(0.62\pm 0.09\) & ND & \(0.55\pm 0.01\) & \(0.46\pm 0.01\) & ND & ND & ND & ND \\ \hline
**Phenois \({}^{\star}\)** & & & & & & & & & & & & \\ \(\sim\)Gaisicoal & ND & & & & & & & & & & & & \\ \(\sim\)Froscol & \(\mathcal{O}70\pm 2\) & \(65\pm 4\) & \(13\) & \(150\pm 3\) & \(199\pm 3\) & \(171\pm 3\) & \(185\pm 3\) & \(116\pm 6\) & \(124\  
\begin{table}
\begin{tabular}{l c c c c c c c c c c} \hline \hline \multicolumn{1}{c}{**Compounds**} & \multicolumn{1}{c}{**Samples \({}^{\bullet}\)**} & \multicolumn{1}{c}{**M1 (control)**} & \multicolumn{1}{c}{**M2 (control)**} & \multicolumn{1}{c}{**MFC1**} & \multicolumn{1}{c}{**MFC2**} & \multicolumn{1}{c}{**MFL1**} & \multicolumn{1}{c}{  
\begin{table}
\begin{tabular}{l c c c c c c c c c c} \hline \hline \multicolumn{1}{c}{**Compounds**} & \multicolumn{1}{c}{**Samples \({}^{\star}\)**} & \multicolumn{1}{c}{**Samples \({}^{\star}\)**} & \multicolumn{1}{c}{**Samples \({}^{\star}\)**} & \multicolumn{1}{c}{**M1 (control)**} & \multicolumn{1}{c}{**M2 (control)**} & \multicolumn{1}{c}{**MFC1**} & \multicolumn{1}{c}{**MFC2**} & \multicolumn{1}{c}{**MFL1**} & \multicolumn{1}{c}{**MFL2**} & \multicolumn{1}{c}{**MCC1**} & \multicolumn{1}{c}{**MCC2**} & \multicolumn{1}{c}{**MCL1**} & \multicolumn{1}{c}{**MCC2**} \\ \hline
**Edges \({}^{\star}\)** & & & & & & & & & & & \\ \hline Methyl protonate & \(14\pm 5\) & \(25\pm 2\) & \(55\pm 5\) & \(41\pm 1\) & \(12\pm 2\) & \(24\pm 1\) & \(32\pm 3\) & \(36\cdot 7\pm 0.4\) & \(10\pm 7\) & \(22\pm 1\) \\ Propyl acetate & \(15\pm 3\) & \(16\pm 1\) & \(30\pm 2\) & \(48\pm 1\) & \(20\pm 0.5\) & \(1.79\pm 0.08\) & \(73\pm 3\) & \(51\pm 2\) & \(12\pm 0.6\) & \(1.0\pm 0.1\) \\ Methyl 2-methylbutanoate & \(31\pm 07\) & \(36\pm 1\) & \(30\pm 0.5\) & \(31\pm 0\) & \(31\pm 0\) & \(31\pm 0\) & \(33\pm 0\) & \(33\pm 0\) & \(2.8\pm 0.3\) & \(1.7\pm 0.7\) & \(2.6\pm 0.2\) \\ Ethyl abundance & ND & ND & ND & ND & ND & ND & ND & ND & \(9\pm 3\) & ND \\ Propyl propionate & \(16\pm 3\) & \(13\pm 1\) & \(98\pm 8\) & \(94\pm 2\) & \(49\pm 0.7\) & \(47\pm 0.04\) & \(10\pm 4\) & \(98\pm 10\) & \(4\pm 1\) & \(83\pm 0.08\) \\ Methyl perinatone & ND & ND & \(05\pm 0.02\) & \(123\pm 0.1\) & \(52\pm 4\) & \(66\pm 1\) & \(41\pm 0.02\) & \(0.1\pm 0.1\) & \(59\pm 19\) & \(423\pm 0.1\) \\ Ethyl pentanoate & ND & ND & \(09\pm 1\) & ND & \(36\pm 01\) & \(0.34\pm 0.01\) & \(0.52\pm 0.01\) & \(0.1\pm 0.1\) & ND & \(124\pm 0.1\) \\ Methyl hexanoate & ND & ND & ND & \(39\pm 01\) & \(40\pm 5\) & \(42\pm 1\) & \(0.13\pm 0.05\) & \(0.59\pm 0.03\) & \(65\pm 19\) & \(11\pm 0.4\) \\ Proyl pentanoate & ND & ND & \(03\pm 0.4\) & \(70\pm 0.1\) & \(74\pm 0.4\) & \(39\pm 0.4\) & \(0.2\pm 0.2\) & ND & \(17\pm 2\) & \(76\pm 0.2\) \\ Ethyl hexanoate & ND & ND & ND & \(037\pm 00\) & \(02\pm 0.00\) & ND & \(04\pm 03\) & ND & \(117\pm 0.9\) & \(69\pm 0.08\) \\ Methyl heptanoate & ND & ND & ND & ND & \(04\pm 05\) & ND & ND & ND & \(03\) \\ Propyl hexanoate & ND & ND & ND & \(049\pm 00\) & ND & ND & ND & \(08\pm 0.1\) & ND \\ Methyl cycloheancarboxylic & \(0.12\pm 0.06\) & \(01.04\pm 0.00\) & \(119\pm 0.4\) & \(37\pm 2\) & \(351\pm 0.01\) & \(01\pm 0.1\) & \(02\pm 0.2\) & \(80\pm 0.7\) & \(26\pm 1\) & \(31\pm 1\) \\ Ethyl cyclohexanecarboxylic & \(0.19\pm 0.04\) & ND & \(5.2\pm 0.1\) & \(182\pm 0.1\) & \(0.29\pm 0.03\) & ND & \(02\pm 0.2\) & \(6.1\pm 0.4\) & \(223\pm 0.7\) & \(83\pm 0.7\) \\ Benzyl propionate & ND & ND & \(16\pm 01\) & \(12\pm 0.01\) & \(0.57\pm 0.01\) & \(02\pm 0.03\) & \(089\pm 0.02\) & \(13.3\pm 0.7\) & \(08\pm 0.03\) & \(081\pm 0.05\) \\ 2-Phenylly acetate & \(18\pm 01\) & \(230\pm 07\) & ND & \(2.08\pm 0.01\) & \(173\pm 0\) & ND & \(479\pm 0.01\) & ND & \(469\pm 0.02\) & \(2.28\pm 0.02\) \\ Methyl hyd hydcominate & \(0.07\pm 0.03\) & \(0.07\pm 0.01\) & \(0.3\pm 0.2\) & \(0.27\pm 0.04\) & \(0.03\pm 0.03\) & \(0.01\pm 0.01\) & \(052\pm 0.05\) & \(0.38\pm 0.01\) & \(06\pm 0.01\) & \(092\pm 0.09\) \\ Benzyl pentanoate & ND & ND & ND & \(0.64\pm 0.03\) & \(0.2\pm 0.2\) & ND & ND & \(054\pm 0.00\) & \(0.3\pm 0.1\) \\ \hline
**Phenois \({}^{\star}\)** & & & & & & & & & & & \\ \(\circ\)-Gaisicol & ND & \(42\pm 2\) & \(39\pm 0.3\) & \(186\pm 07\) & \(39\pm 0.8\) & \(490\pm 0.7\) & \(58\pm 9\) & \(12\pm 1\) & \(20\pm 0.4\) \\ \(\circ\)-Focusol & \(64\pm 6\) & \(78\pm 5\) & \(99\pm 9\) & \(96\pm 1\) & \(164\pm 3\) & \(185\pm 3\) & \(13\pm 2\) & \(131\pm 20\) & \(131\pm 9\) & \(128\pm 3\) \\ Phenol & \(0.58\pm 0.03\) & \(0.8\pm 0.1\) & \(20\pm 1\) & \(181\pm 02\) & \(39\pm 0.1\) & \(58\pm 0.4\) & \(21.2\pm 0.8\) & \(24\pm 3\) & \(12\pm 1\) & \(135\pm 0.07\) \\ \(\circ\)-Fhenyl guaicol & \(0.08\pm 0.00\) & \(0.99\pm 0.00\) & \(0.28\pm 0.04\) & \(0.30\pm 0.00\) & \(0.19\pm 0.0\) & \(0.18\pm 0.03\) & \(0.35\pm 0.03\) & \(0 When the whole dataset was considered, PCA showed a clear separation between the unspoiled control samples and spoiled samples, which were located in different quadrants (Figure 5a).