## 3 Results  
It was found that the histological structures of the kidney tissues removed from the control group rates had a normal appearance. Tubular dilation, hydropic degeneration in the tubular cells, shrinkage in the glomeruli and congestion were observed in the kidney tissues removed from the rats that were administered iron. Although similar findings were obtained in the zinc group, congestion was not observed. The histopathological changes were similar in the groups which were administered juglone together with Fe and Zn, although the tubular dilation and degeneration in the tubular cells were not as severe. When the severity of the damage was scored, the highest level of damage was in the Fe group (++), followed by the Fe-juglone (++) and Zn (++) groups. It was found that there was mild damage in the Zn-juglone group. In the immunohistochemical examinations, the apoptotic cell count was found to be 69.50 \(\pm\) 4.20 on average in the Fe group, 41.25 \(\pm\) 2.75 on average in the Zn group, 43.0 \(\pm\) 3.55 on average in the Fe-juglone group, 26.25 \(\pm\) 3.55 on average in the Zn-juglone group, and 14.75 \(\pm\) 2.55 on average in the control group (Figure 1). The conformity of the parameters in all the study groups to the normal distribution was evaluated with the Kolmogorov-Smirnov test. The changes observed in the relevant parameters of the study groups were made using one-way analysis of variance (one-way ANOVA) and multiple comparisons were made according to the Bonferroni test. Values of \(p<\) 0.05 were considered statistically significant. The apoptotic-cell-count data and the statistical-analysis results (Table 1) are presented. The difference between the groups in terms of the total apoptotic-cell count was statistically significant (\(p\) = 0.001). The difference between the control group, Zn-juglone group, Zn group, Fe-juglone group and Fe group was statistically significant and the apoptotic-cell count increased in the Fe group (\(p\) = 0.001). The difference between the groups containing juglone and the Fe group and Zn group were statistically significant and a decrease in the apoptotic-cell count was detected in the groups containing juglone (\(p\) = 0.001). Figure 1: **(a)** Fe group, kidney, H&E, tubular dilation (arrow), \(\times\)40; **(b)** Zn group, kidney, H&E, tubular dilation (arrow), \(\times\)40; **(c)** Control group, Kidney, H&E, \(\times\)40; **(d)** Fe group, apoptotic cells in kidney (arrow), TUNEL, \(\times\)40; **(e)** Fe–juglone group, apoptotic cells in kidney (arrow), TUNEL, \(\times\)40; **(f)** Zn group, apoptotic cells in kidney (arrow),TUNEL, \(\times\)40. It was found that the histological structure of the liver tissues removed from the control group rats had a normal appearance. Hydropic vacuolar degeneration, mononuclear cell infiltration and dilation in the sinusoids were observed in the liver tissues of the rats that were administered Fe and Zn, while focal necrosis was observed in the Fe group. When the severity of the damage was scored, the highest level of damage was in the Fe group (+++), followed by the Zn and Zn-juglone groups (++). It was found that there was a mild damage in the Zn-juglone group (Figure 2). In terms of the apoptotic cell count, the Fe group (51.66 \(\pm\) 2.91) demonstrated the highest levels, while the Zn group (50.60 \(\pm\) 2.40) demonstrated the second-highest levels. It was found that there no significant difference between the Fe-juglone group (14.00 \(\pm\) 3.80), the Zn-juglone group (13.60 \(\pm\) 3.04) and the control group (12.60 \(\pm\) 1.81) (Table 2 and Figure 3). The obtained results were expressed as arithmetic mean (X) and standard deviation (SD) and the statistical analysis was performed using the SPSS 20.0 program. The conformity of the parameters in all the study groups to the normal distribution was evaluated with the Kolmogorov-Smirnov test.