## Materials and Methods
### Plant, Spices, and Vegetable Extracts
#### 2.1.3 Antioxidant Activity  
The antioxidant activity related with the chelating power to different free radicals (%) was firstly measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPPH) free radical scavenging method, described by [PERSON] et al. [16] and [PERSON] et al. [17]. Each extract was diluted in a 1000 ppmsolution with water or ethanol. A DPPH radical solution was prepared with 0.0063 g of DPPH in 250 mL of ethanol (99%). Then, 3.9 mL of DPPH radical solution was added to 100 \(\upmu\)L of sample. After incubation in the dark at room temperature for 30 min, the absorbance was measured at 515 nm. The chelating activity percent was calculated using the next formula: ((Abs DPPH (initial) - Abs DPPH (final \"added sample\"))/Abs DPPH (initial)) \(\times\) 100.  
Secondly, the radical cation scavenging capacity against ABTS+ radical was measured as by [PERSON] et al. [18]. A 1000 ppm solution of each extract was mixed with water or ethanol. ABTS radical cations were prepared by reacting 7 mM ABTS (2,2-Azinobis (3-ethylbenzothizolin)-6-sulphonic acid) with 2.45 mM potassium persulphate (1:1, _v/v_), pH = 7.4. This solution was diluted with distilled water to an absorbance of 0.7000 at 734 nm. In total, 1 mL of ABTS solution was added to 100 \(\upmu\)L of sample. After 2 min incubation, the absorbance was measured at 734 nm. The chelating activity percent was calculated using the next formula: ((Abs ABTS (initial) - Abs ABTS (final \"added sample\"))/Abs ABTS (initial)) \(\times\) 100.  
Otherwise, the hydrophilic antioxidant capacity was measured using the ORAC (Oxygen Radical Absorbance Capacity) method described by [PERSON] et al. [19]. The reaction was carried out in a 0.075 M phosphate buffer (pH 7.0). For this, 20 \(\upmu\)L of sample, at different concentrations, and Trolox standard solutions (6.25, 12.5, 25, 50 \(\upmu\)M) were pipetted into the wells of a 96-well black microplate. When the microplate was ready, 200 \(\upmu\)L of 0.04 \(\upmu\)M fluorescein were dispensed into each well. Samples were incubated for 15 min at 37 \({}^{\circ}\)C in the dark and the reaction was started by adding 20 \(\upmu\)L of 40 mM AAPH to each well. The fluorescence decay was measured every 2 min at 37 \({}^{\circ}\)C using the microplate reader, Biotek Synergy HT (Biotek Intruments, Winooski, VT, USA), at a 485 nm excitation and 538 nm emission until zero fluorescence was detected and a UV2 spectrophotometer (Pye Unicam Ltd, Cambridge, UK) at different wavelengths depending on the method to be performed. All samples were prepared in triplicate and at a minimum of three different concentrations. The antioxidant activity of the sample is expressed as \(\upmu\)M of Trolox equivalents (TE) per g of extract, with the following formula: (C \(\times\) DF)/a, where \(C\) is obtained from the area under the fluorescence decay curve of fluorescein in the presence of the sample (AUC net = AUC sample - AUC blank); \(DF\) is the dilution factor; and \(a\) is the weight of the sample.  
The ferric ion reducing antioxidant power assay (FRAP) was also carried out following the method described by [PERSON] and [PERSON] [20] with some modifications. Each extract was diluted in a 1000 ppm solution using water or ethanol. Then, the FRAP reagent was prepared with 20 mL of 300 mmol/L acetate buffer, pH = 3.6, 2 mL 20 mmol/L FeCl\({}_{3}\) \(\cdot\) 6 H\({}_{2}\)O, and 2 mL 10 mmol/L TPTZ (2,4,6-tripyrid-s-triazine) in 40 mmol/L HCl. After, 1 mL of the FRAP reagent was mixed with 100 \(\upmu\)L of sample or a standard solution of 500 \(\upmu\)M Trolox in plastic cuvettes. After incubation for 4 min, the absorbance was measured at 593 nm. The antioxidant power was expressed as \(\upmu\)M Trolox equivalents (TE) per g extract.