# Separation of Albumin from Bovine Serum Applying Ionic-Liquid-Based Aqueous Biphasic Systems
## 4 Conclusions
Figure S12: Tetrabutylammonium acetate, [N\({}_{4444}\)][Ac], \({}^{13}\)C NMR (D\({}_{2}\)O, 75.47 MHz, [ppm]): 181.07; 57.99; 23.33; 23.05; 19.09; 12.82. Figure S13: Tetrabutylphosphonium acetate, [P\({}_{4444}\)][Ac], \({}^{1}\)H NMR (D\({}_{2}\)O, 300 MHz, [ppm]): 1.H NMR (D\({}_{2}\)O, 300 MHz, [ppm]): 1.H NMR (D\({}_{2}\)O, 300 MHz, [ppm]): 2.17 (m, 8H); 1.91 (s, 3H); 1.50 (m, 16H); 0.93 (t, 12H). Figure S14: Tetrabutylphosphonium acetate, [P\({}_{4444}\)][Ac], \({}^{13}\)C NMR (D\({}_{2}\)O, 75.47 MHz, [[ppm]): 180.83; 23.15; 22.63; 17.90; 17.26; 12.522. Table S1: Experimental weight fraction data for the binodal curves of the ABS formed by [C\({}_{4}\)mim][MES] (1) + K\({}_{2}\)HPO\({}_{4}\)/KH\({}_{2}\)PO\({}_{4}\) (2) + H\({}_{2}\)O (3) at (25 \(\pm\) 1) \({}^{\circ}\)C and pH 7. Table S2: Experimental weight fraction data for the binodal curves of the ABS formed by [N\({}_{4444}\)][MES] (1) + K\({}_{2}\)HPO\({}_{4}\)/KH\({}_{2}\)PO\({}_{4}\) (2) + H\({}_{2}\)O (3) at (25 \(\pm\) 1) \({}^{\circ}\)C and pH 7. Table S3: Experimental weight fraction for the binodal curves of the ABS formed by [P\({}_{4444}\)][MES] (1) + K\({}_{2}\)HPO\({}_{4}\)/KH\({}_{2}\)PO\({}_{4}\) (2) + H\({}_{2}\)O (3) at (25 \(\pm\) 1) \({}^{\circ}\)C and pH 7. Table S4: Experimental weight fraction for the binodal curves of the ABS formed by [N\({}_{4444}\)][Arg] (1) + K\({}_{2}\)HPO\({}_{4}\)/KH\({}_{2}\)PO\({}_{4}\) (2) + H\({}_{2}\)O (3) at (25 \(\pm\) 1) \({}^{\circ}\)C and pH 7. Table S5: Experimental weight fraction for the binodal curves of the ABS formed by [P\({}_{4444}\)][Ac] (1) + K\({}_{2}\)HPO\({}_{4}\)/KH\({}_{2}\)PO\({}_{4}\) (2) + H\({}_{2}\)O (3) at (25 \(\pm\) 1) \({}^{\circ}\)C and pH 7. Table S6: Experimental weight fraction for the binodal curves of the ABS formed by [C\({}_{4}\)mim][Ac] (1) + K\({}_{2}\)HPO\({}_{4}\)/KH\({}_{2}\)PO\({}_{4}\) (2) + H\({}_{2}\)O (3) at (25 \(\pm\) 1) \({}^{\circ}\)C and pH 7. Table S7: Experimental weight fraction for the binodal curves of the ABS formed by [P\({}_{4444}\)][Ac] (1) + K\({}_{2}\)HPO\({}_{4}\)/KH\({}_{2}\)PO\({}_{4}\) (2) + H\({}_{2}\)O (3) at (25 \(\pm\) 1) \({}^{\circ}\)C and pH 7. Table S8: Experimental weight fraction for the binodal curves of the ABS formed by [P\({}_{4444}\)][Ac] (1) + K\({}_{2}\)HPO\({}_{4}\)/KH\({}_{2}\)PO\({}_{4}\) (2) + H\({}_{2}\)O (3) at (25 \(\pm\) 1) \({}^{\circ}\)C and pH 7. Table S9: Correlation parameters of Equations (1) (2) and (2) used to describe the experimental binodal curve of the system. Table S10: Data of TLs and TLLs for the systems composed of IL + K\({}_{2}\)HPO\({}_{4}\)/KH\({}_{2}\)PO\({}_{4}\) buffer at pH 7 + H\({}_{2}\)O. [IL] and [salt] represents the compositions of IL and salt, respectively and content of water corresponds to the amount required to reach 100 wt%. Figure S15: Phase diagrams for the systems composed of IL + K\({}_{2}\)HPO\({}_{4}\)/KH\({}_{2}\)PO\({}_{4}\) buffer at pH 7 + H\({}_{2}\)O at 25 \({}^{\circ}\)C: [C\({}_{4}\)mim][MES] (\(\bullet\)); [N\({}_{4444}\)][MES] (\(\bullet\)); [N\({}_{4444}\)][Arg] (\(\blacktriangle\)); [P\({}_{4444}\)][Arg] (\(\blacklozenge\)); [C\({}_{4}\)mim][Ac] (\(\blacklozenge\)); [N\({}_{4444}\)][Ac] (\(\blacklozenge\)); [P\({}_{4444}\)][Ac] (\(\blacklozenge\)). Table S11: Extraction efficiencies (\(E\%\)) and yield of ABS composed of IL + K\({}_{2}\)HPO\({}_{4}\)/KH\({}_{2}\)PO\({}_{4}\) pH 7 + H\({}_{2}\)O for pure BSA. Table S12: Yield and purity of BSA from bovine serum by ABS composed of [N\({}_{4444}\)][Ac] + K\({}_{2}\)HPO\({}_{4}\)/KH\({}_{2}\)PO\({}_{4}\) pH 7 + H\({}_{2}\)O. Figure S16. SE-HPLC Chromatogram with bovine serum dilution 1:15 (\(v\):) (---), pure BSA 0.8 g-L\({}^{-1}\) (---) and top-phase of the system [N\({}_{4444}\)][Ac] + K\({}_{2}\)HPO\({}_{4}\)/KH\({}_{2}\)PO\({}_{4}\) buffer at pH 7 + bovine serum 1:15 (\(v\):) (---). Conceptualization, M.G.F. ; investigation, A.F.C.S.R., M.R.A., and M.S. ; resources, J.A.P.C. and M.G.F. ; writing--original draft preparation, A.F.C.S.R. ; writing--review and editing, M.R.A., J.A.P.C., and M.G.F.