## 4 Conclusions  
From the results obtained in this study it can be concluded that both time and solvent have a significant impact on the recovery of antioxidant compounds when conventional extraction is used, obtaining the highest values of phenolic compounds, chlorophyll a, chlorophyll b, and carotenoids when the mixture EtOH/H\({}_{2}\)O was used for 180 min. Moreover, a strong relationship of TEAC values with total phenolic compounds, carotenoids, and chlorophyls was found while ORAC values were positively correlated with carotenoids. Both PEF treatment and extraction time had a statistically significant effect on the recovery of antioxidant compounds when the EtOH/H\({}_{2}\)O mixture was used, showing a considerable reduction in the extraction time required to recover polyphenols, carotenoids, and chlorophyls compared to those of conventional treatment. When the impact of PEF and the DMSO/H\({}_{2}\)O mixture was evaluated, it was found that both the treatment and the extraction time had a statistically significant effect, reducing the extraction times compared to the conventional treatment; however, in this case the maximum content of antioxidant compounds was lower than that observed for EtOH/H\({}_{2}\)O. TAC values were also increased after PEF treatment, mainly due to an increase in the extraction of antioxidant compounds. In addition, the maximum efficiency values were observed at 5 and 15 min for the two solvents, with a greater effect on the extraction of carotenoids and total phenolic compounds. In the future, the implementation of PEF in the extraction of antioxidant bioactive compounds from microalgae would be interesting, as it could be a promising and environmentally sustainable technology.  
Conceptualization, E.F., H.B. and F.J.B.; methodology, F.R.-M., F.J.M.-Q. and N.P.; formal analysis, F.R.-M., F.J.M.-Q. and N.P.; software, F.R.-M. and F.J.M.-Q.; resources, F.J.B., E.F. and H.B.; writing--original draft preparation, F.R.-M., F.J.M.-Q., N.P., Y.P. and F.J.B.; writing--review and editing, F.J.B., E.F. and H.B.; supervision, F.J.B., E.F. and H.B.; funding acquisition, F.J.B., E.F. and H.B. All authors have read and agreed to the published version of the manuscript.  
This research was funded by the University of Valencia through the project OTR2021-21736 INVES, supported by the University of Vigo. Moreover, it was partially funded by the EU Commission and BBI-JU Horizon H2020, through the AQUABiOPRO-FIT project (aquaculture and agriculture biomass side stream proteins and bioactives for feed, fitness and health promoting nutritional supplements) grant number 790956.  
The authors would like to acknowledge [PERSON] (Ecospirulina company) for providing the microalgae samples to carry out the experiments. They also thank Generalitat Valenciana for financial support (IDIFEDER/2018/046--Procesos innovadores de extracciom y conservacion: pulsos electricos y fluidos supercriticos) through the European Union ERDF funds (European Regional Development Fund). Moreover, [PERSON] also would like to acknowledge the pre-PhD scholarship program of University of Valencia \"Attaccio de Talent\".  
The authors declare no conflict of interest.