## 3 Results
### Gas Hydrate Inhibition Study  
The gas hydrate inhibiting property of a chemical could be determined from the HLVE data collected during the formation and dissociation of CO\({}_{2}\) hydrates in the rocking cell hydrate reactor (Figure 1). The evaluation of thermodynamic gas hydrate suppression considered the findings of equilibrium points where hydrate, liquid, and vapor phases are coexisting simultaneously [15]. The accuracy of the results was validated through measurement and comparison of the data with earlier works of literature [15; 45]. In this work, the CO\({}_{2}\) equilibrium points were taken by altering the pressure from 2 MPa to 4 MPa in the reactor cells. This range of pressure was determined based on the typical scenario of CO\({}_{2}\) dominated oil and gas pipelines in the Energy industry [46]. The equilibrium temperature obtained under this pressure range was between 273.15 and 283.15 K. Table 3 tabulates the HLVE findings of the current work, while Figure 7 attempts to display the results graphically. \begin{table}
\begin{tabular}{c c c c c c c c c c} \hline \hline \multicolumn{2}{c}{**Water**} & \multicolumn{2}{c}{**TEAAC:Glycerol 5 wt \%**} & \multicolumn{2}{c}{**TEAAC:MEG 5 wt \%**} & \multicolumn{2}{c}{**TEAB:MEG 5 wt \%**} & \multicolumn{2}{c}{**TEAB:Glycerol 5 wt \%**} \\ \hline
**T/K** & **P/MPa** & **T/K** & **P/MPa** & **T/K** & **P/MPa** & **T/K** & **P/MPa** & **T/K** & **P/MPa** \\ \hline
277.00 & 2.00 & 276.34 & 2.30 & 275.14 & 2.00 & 275.80 & 2.00 & 274.73 & 2.00 \\
278.80 & 2.50 & 278.30 &  
It was observed from Figure 7 that all four DESs formulated managed to shift the equilibrium boundary of water to the left where lower temperature and higher pressure were required to form hydrates. The shift proved the thermodynamic gas hydrate inhibiting the behavior of DESs. In addition, TEAB:Glycerol showed the maximum change with the lowest equilibrium point at 20 bar and 274.73 K, followed by TEAB:MEG. The gas hydrate inhibition arranged from the highest to lowest would be TEAB:Glycerol > TEAB:MEG > TEAAC:Glycerol > TEAAC:MEG. DESs have great potential to be effective green Thermodynamic Hydrate Inhibitors as they have the functional groups capable of forming hydrogen bonds with water [35; 36]. The reliability of the data was validated through statistical analysis, and the results are tabulated in Table 4.  
Based on the regression analysis, the R\({}^{2}\) and adjusted R\({}^{2}\) values obtained for the model on the experimental data were generally above 0.90. According to the literature [47; 48], the regression statistics can be considered as acceptable with an R\({}^{2}\) value > 0.75 and adjusted R\({}^{2}\) value > 0.5. In terms of standard error, the values were all below 0.68, signifying the reliability of the data. The significance F values were also below the 0.05 mark, with 0.0055 to be the highest. These values indicated that the set of independent variables were statistically significant. The superiority of TEAB:Glycerol-based DES over the rest of the DESs could be due to the nature of the pure components [34]. The previous works on TEAB and Glycerol have shown that each of the chemicals has functional hydrate inhibition [11; 12; 15]. It was proven in this study that the formation of DES using TEAB and Glycerol showed synergistic nature, leading to enhanced results. From the pattern observed, it was agreed that as TEAB was superior to TEAAC and Glycerol was superior to MEG in inhibiting gas hydrate inhibition, the combination of the compounds also showed similar nature to the pure compound. However, it is to be noted that not all DESs will show the same characteristics as some mixtures may show antagonistic behavior. A fundamental requirement for a chemical to be an effective THI is the potential to form hydrogen bonds with host water molecules, leading to the demand of higher pressure and lower temperature conditions to maintain the hydrate lattice structures.