## 3 Results and Discussion
### Antibacterial and Antifungal Activity of Bacillus amyloliquefaciens \(\mathrm{M}\)
[36] argued that fatty acid methyl ester extract showed moderate  
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & & \multicolumn{4}{c}{**d\({}_{\text{inhibition zone}}\), mm**} \\ \cline{2-6} \multicolumn{1}{c}{_Bacillus amyloidateaciens_**} & \multicolumn{4}{c}{_Sclerotinia sclerotorum_**} **var sclerotorum**} **ATCC **  
antifungal activity against two _Aspergillus_ spp. Moreover, ethyl hexadecanoate had antimicrobial, antioxidant, and pesticidal activities [37]. The least observed in either of the bacterial extracts were octyl hexadecanoate, diethyl phthalate, 2-phenyltridecane, methyl 10-methyl undecanoate, 5-octadecene, octadecane, and methyl tetradecanoate that had antimicrobial, antioxidant, and anticancer activities [34]. The experimental results of the inhibitory action of _Bacillus amyloliquefaciens_ M in the anaerobic cultivation of _Bacillus amyloliquefaciens_ M at 30 \({}^{\circ}\)C are presented in Table 4. \begin{table}
\begin{tabular}{c c c c c} \hline \hline \multicolumn{2}{c}{_Bacillus amyloliquefaciens_ M} & \multicolumn{3}{c}{**Test Microorganisms**} \\ \cline{3-5} \multicolumn{1}{c}{**Static Cultivation**} & \multirow{2}{*}{_Aspergillus niger_ ATCC 1015,_} & _Aspergillus niger_ & _Aspergillus_ & _Aspergillus_ \\ \multicolumn{1}{c}{**48 h**} & & **ATCC 1015,** & **azumori ATCC** & **flavus ATCC** \\ \cline{3-5} \multicolumn{1}{c}{**48 h**} & & **3.0 \(\times\) 10\({}^{5}\)** & **22342,** & **9643,** \\ \cline{3-5} \multicolumn{1}{c}{} & & **cfu/cm\({}^{3}\)** & **1.2 \(\times\) 10\({}^{5}\)** & **1.1 \(\times\) 10\({}^{5}\)** \\ \cline{2-5} \multicolumn{1}{c}{} & & **cfu/cm\({}^{3}\)** & **cfu/cm\({}^{3}\)** \\ \hline \multirow{2}{*}{\begin{tabular}{c} LBG broth \\ pH = 4.5 \\ \end{tabular} } & \begin{tabular}{c} ASN \\ \end{tabular} & 23.17 \(\pm\) 0.24 & 10.17 \(\pm\) 0.24 & 10.17 \(\pm\) 0.24 \\ \cline{2-5} \multicolumn{1}{c}{} & \begin{tabular}{c} CS \\ \end{tabular} & 23.33 \(\pm\) 0.47 & 12.33 \(\pm\) 0.47 & 10.33 \(\pm\) 0.47 \\ \cline{2-5} \multicolumn{1}{c}{} & \begin{tabular}{c} B \\ \end{tabular} & 26.33 \(\pm\) 0.47 & 10.17 \(\pm\) 0.24 & 10.33 \(\pm\) 0.47 \\ \hline \multirow{2}{*}{\begin{tabular}{c} LBG broth \\ pH = 5 \\ \end{tabular} } & \begin{tabular}{c} ASN \\ \end{tabular} & 25.67 \(\pm\) 0.47 & 40.67 \(\pm\) 0.47 & 25.17 \(\pm\) 0.24 \\ \cline{2-5} \multicolumn{1}{c}{} & \
\begin{tabular}{c} CS \\ \end{tabular} & 28.33 \(\pm\) 0.47 & 20.50 \(\pm\) 0.41 & 24.17 \(\pm\) 0.24 \\ \cline{2-5} \multicolumn{1}{c}{} &
\begin{tabular}{c} B \\ \end{tabular} & 28.17 \(\pm\) 0.24 & 30.33 \(\pm\) 0.47 & 28.50 \(\pm\) 0.41 \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Antibytopathogenicic activity of _B. amyloliquefaciens_ M against phytopathogenicic fungi in static cultivation at 30 \({}^{\circ}\)C.  
\begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline \multicolumn{2}{c}{_B_} & 18.33 \(\pm\) 0.47 & - & 9.50 \(\pm\) 0.41 & 26.50 \(\pm\) 0.41 & 20.17 \(\pm\) 0.24 & 32.50 \(\pm\) 0.41 & 18.50 \(\pm\) 0.41 \\ \hline \multirow{2}{*}{ \begin{tabular}{c} ASN \\ \end{tabular}  } & \multirow{2}{*}{18.17 \(\pm\) 0.24} & \multirow{2}{*}{-} & 9.33 \(\pm\) 0.47 & 23.17 \(\pm\) 0.24 & 18.17 \(\pm\) 0.24 & 28.17 \(\pm\) 0.24 & 20.50 \(\pm\) 0.41 \\ \hline \multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{\multicolumn{2}{c}{  
_Bacillus amyloidateaciens_ M exhibited high antifungal activity, with the largest inhibition zones after 48 h of cultivation being determined at medium LBG broth and pH = 7.0 for _Aspergillus niger_ ATCC 1015 and _Aspergillus avamori_ ATCC 22342 and pH = 6.0-8.0 for _Aspergillus flavus_ ATCC 9643, and at MPB broth and at pH = 6.0 for all three phytopathogenic test microorganisms (Table 4). Large inhibition zones were determined in the dynamic cultivation of the strain in the laboratory bioreactor (Table 5).