# New Local Drug Delivery with Antibiotic in the Nonsurgical Treatment of Periodontitis--Pilot Study
## 3 Materials and Methods  
Ten patients aged 24 to 56 (the average age was 39.5, 5 males, 5 females) with general periodontitis (loss of CAL on interproximal surfaces, CAL in >30% of teeth, with moderately symmetric bone loss in pantomographic image) were qualified for the study. The patients were healthy nonsmokers who had not used antibiotics within the period of 6 months prior to the study, were not allergic to penicillin, and had at least 10 teeth in the jaw. The excluded criteria were also diabetes mellitus and hypertension.  
The second stage of periodontitis was diagnosed in 1 case, the third stage in 6 cases, and the fourth in 3 cases. Degree distribution was 7 from B and 3 from C. All these individuals provided written consent for the proposed treatment protocol. The study was granted approval KB-600/2019 by the Bioethical Committee of Wroclaw Medical University.  
The procedure would begin with providing instructions on oral hygiene (the obtained average API value was below 20%). Subsequently, machine SRP (ultrasonic scaling with subgingival tips) under nerve block anesthesia in the mandible was performed during two visits. Examination of clinical parameters was conducted by one standardized periodontitis (TK). The examinations determined parameters such as bleeding on probing (BoP), clinical attachment location (CAL), and periodontal depth (PD) in six measurement points around each tooth, except for the third molars. Then, microbiological material was collected from two symmetric pockets with similar PD, located on both sides of the dental arch; sterile filter paper was placed in the pockets for 30 s, and the samples were then transferred to transport test-tubes. During the same visit, the periodontitis (ASJ) conducted analogous SRP under anesthesia in the jaw. At the end of the visit, a third periodontitis (JZ) introduced Gelcide into the periodontal pockets, in accordance with the instructions of the manufacturer, applying randomization to odd-numbered patients in the order of their applications on the left, and even-numbered patients on the right. The randomization was known to only one person (JZ). The patients were informed about the possibility of attending an additional follow-up visit, if any adverse effects associated with the conducted treatment were noticed. If no such effects occurred, the follow-up visits took place one, four, and eight weeks after the initial examination. Clinical examination was conducted again at the last follow-up visit, and the material for microbiological tests (TK) was collected from the same pockets.  
During microbiological testing, the PET deluxe test was applied. The test uses the PCR method in real time that makes it possible to quantify the total bacterial count (TBC) in the pocket as well as the red-complex bacteria (_Porphyromonas gingivalis_--P.g., _Treponema denticola_--T.d., and oraz _Tannerella forsythia_--T.f.), _Aggregatibacter actinomycetemitants_--A.a.), orange-complex bacteria (_Fusobacterium nucleatum_--F.n., _Prerotella intermedia_--P.i., and _Micromonas micros_--P.m., _Eubacterium nodatum-E.n._), and green-complex bacteria (_Capnocyotophaga gingivalis_--C.g.).  
Due to lack of normality of distribution of the analyzed variables, nonparametric tests were used in statistical analysis: the Mann-Whitney U test for nonrelated variables and the Wilcoxon T-test for related variables and for groups below 25 participants. \(p\leq 0.05\) was adopted as the significance level. The Statistica package, version 13.1, was used.