## 3 Results  
The study of micromycetes in sediments/soil of the Brestovska Cave was carried out at one outdoor and five indoor sites using the culturing procedure (PDA medium, incubation at 5 and 24 \(\pm\) 0.5 \({}^{\circ}\)C) (Figure 1). The phenotypic analysis of the obtained fungi allowed for their classification into 20 different species, and molecular studies confirmed those observations. The obtained fungi belong to four phyla (Ascomycota--75% of isolates, Mortierellomycota--15%, Mucornycota--5%, and Basidiomycota--5% isolates) and thirteen families (_Aspergillus_-represent 20% of isolates, _Mortierellaceae_--15% of isolates, _Coniochaetaceae_ and _Microaceae_--each represent 10% of isolates, and each of the following represent 5% of isolates--_Cordcycitiaceae_, _Dermateaceae_, _Hypocreaceae_, _Mrakiaceae_, _Mucoraceae_, _Mycatricaceae_, _Nectriaceae_, _Onygenaceae_, and _Pseudeurotiaceae_. Most of the species belong to filamentous fungi (_Ambomucor seriatoinfatatus_, _Cephalotrichum hinmuleum_, _Chryssoparium merdiarium_, _Cosmospora berkelyana_, _Doratomyces stemoitis_, _Lecanicillium muscarium_, _Leythophora hofmannnii_, _Mortierella elongata_, _Mortierella hyalina_, _Mortierella minutissima_, _Oidiodendron truncatum_, _Paecicomyces farinosus_, _Penicillium camemberti_, _Penicillium chrysogenum_, _Penicillium commune_, _Penicillium swiecickii_, _Pseudogymoascus pannorum_, and _Tridoderma atrovirdide_), although two species belong to basidiomycetous and ascomycetus yeast (_Tusonia pullulans_ and _Trichosporiella cerebiformis_, respectively). The nucleotide sequences of fungal ITS rDNA acquired during this study were deposited in GenBank and assigned accession numbers from OQ073897 to OQ073916. BLAST analysis of these sequences revealed E values of zero, indicating highly significant matches, with identity percentages ranging from 98.98% to 100% and query coverage spanning from 99% to 100%, as detailed in Table 1.  
The density of mycobiota in the Brestovska Cave at 5\({}^{\circ}\)C ranged from 8.32 to 49.92 CFU \(\times\) 10\({}^{2}\) per 1 g of sediment/soil samples inside the underground facility and was 91.65 CFU \(\times\) 10\({}^{2}\) per 1 g of outdoors samples. In the case of the incubation temperature at 24 \({}^{\circ}\)C, the overall concentrations of fungal propagules obtained inside the cave were from 9.16 to 156.66 CFU \(\times\) 10\({}^{2}\) per 1 g, and outside, the concentration was 177.76 CFU \(\times\) 10\({}^{2}\) per 1 g (Figure 2). In the case of incubation at 5 \({}^{\circ}\)C, the highest value of fungal propagule concentration was recorded at site I, although statistically (Tukey HSD test at \(\alpha\leq\) 0.05), this location did not differ significantly from location no. II, III, IV, and VI. In turn, the lowest concentration of fungi was noted at site V, although statistically, this location also did not differ significantly from location no. II, III, IV, and VI. However, significant differences were noted only between locations I and V (\(p\) = 0.004211). Meanwhile, the highest statistically significant density of mycobiota in the Brestovska Cave grown at 24 \({}^{\circ}\)C was recorded at location no. I and II, and the lowest at location no. V (P\({}_{\text{study sites II, V}}\) = 0.000197). Moreover, in three study sites (I, II, and IV) out of six, statistical differences in the number of obtained fungi between the incubation temperatures of the samples were noted (P\({}_{\text{study sites I}}\) = 0.02343, P\({}_{\text{study sites II}}\) = 0.004203, and P\({}_{\text{study sites IV}}\) = 0.034669)--Figure 2.