## 4 Conclusions  
This study reports the chemical composition and the antioxidant activity of the essential oil from the aerial parts of _D. reboudii_ from Algeria. The results show significant differences between the chemical composition of the essential oil from _D. reboudii_ compared to those from other _Daucus_ species. The compounds that mostly characterized _D. reboudii_ were the oxygenated monoterepenes (E)-anethol and estragole. Our study highlighted a possible new chemotype [(E)-anethol chemotype] in _D. reboudii_ species, confirming the chemical polymorphism occurring either in the species or in the _Daucus_ genus. The antioxidant capacity of the oil was evaluated using three complementary assays, namely metal chelating, ABTS**+ and DPPH* assays. The highest antioxidant activity was observed with DPPH* method, where _D. reboudii_ showed a significantly higher activity compared to BHT at 50 and 100 \(\upmu\)g/mL. This result suggests that the essential oil from _D. reboudii_ could have a potential use in the food industry as food preservative. Nevertheless, further studies are needed to explore other bioactivities of _D. reboudii_ essential oil and to elucidate also the composition of non-volatile compounds.  
\begin{table}
\begin{tabular}{c c c c c} \hline \hline  & \multicolumn{4}{c}{**\% Inhibition**} \\ \hline  & \multicolumn{2}{c}{**25 \(\upmu\)g/mL**} & \multicolumn{2}{c}{**50 \(\upmu\)g/mL**} & \multicolumn{2}{c}{**100 \(\upmu\)g/mL**} & \multicolumn{1}{c}{**200 \(\upmu\)g/mL**} \\ \hline
**DPPH*** & & & & \\ _D. reboudii_ & 38.59 \(\pm\) 1.22 & 66.12 \(\pm\) 2.34 * & 77.31 \(\pm\) 0.28 * & 78.45 \(\pm\) 0.21 \\ BHT & 40.43 \(\pm\) 0.05 & 53.18 \(\pm\) 0.51 & 73.91 \(\pm\) 0.11 & 98.44 \(\pm\) 0.06 \\ \(\alpha\)-tocopherol & 91.16 \(\pm\) 0.17 & 92.03 \(\pm\) 0.55 & 93.77 \(\pm\) 0.07 & 97.25 \(\pm\) 0.06 \\
**ABTS*** & & & & \\ _D. reboudii_ & 25.01 \(\pm\) 1.77 & 63.11 \(\pm\) 1.39 & 79.27 \(\pm\) 1.05 & 82.32 \(\pm\) 0.38 \\ BHT & 89.56 \(\pm\) 0.39 & 90.12 \(\pm\) 0.5 & 95.20 \(\pm\) 0.80 & 99.24 \(\pm\) 0.60 \\ \(\alpha\)-tocopherol & 99.62 \(\pm\) 0.10 & 99.89 \(\pm\) 0.09 & 99.97 \(\pm\) 0.15 & 99.99 \(\pm\) 0.10 \\
**Metal chelating** & & & & \\ _D. reboudii_ & 3.91 \(\pm\) 1.14 & 15.66 \(\pm\) 0.02 & 33.61 \(\pm\) 0.30 & 38.77 \(\pm\) 1.31 \\ EDTA & 58.08 \(\pm\) 0.60 & 62.42 \(\pm\) 0.22 & 90.34 \(\pm\) 0.55 & 96.01 \(\pm\) 0.24 \\ \hline \hline \end{tabular}  
* \(p<0.05\) compared to BHT.  
\end{table}
Table 2: Antioxidant activity of _Daucus reboudii_ Coss. essential oil. Values are expressed as means \(\pm\) SD of three parallel measurements.  
**Supplementary Materials:** The following are available online at [[https://www.mdpi.com/2076-341](https://www.mdpi.com/2076-341)]([https://www.mdpi.com/2076-341](https://www.mdpi.com/2076-341)) 7/11/4/1843/s1.
* [1]**Author Contributions:** Conceptualization, T.S. and H.B.; methodology, T.S., M.O., G.F.; software, M.O. and G.F.; validation, H.B., G.F. and G.P.; formal analysis, T.S.; investigation, H.B.; resources, G.F. and H.B.; data curation, G.P.; writing--original draft preparation, T.S. and H.B.; writing--review and editing, G.P.; visualization, G.P.; supervision, G.F. and H.B.; project administration, H.B. and G.F.; funding acquisition, H.B. All authors have read and agreed to the published version of the manuscript.
* [2]**Funding:** This research was funded by the Ministry of Higher Education and Scientific Research of Algeria.
* [3]**Institutional Review Board Statement:** Not applicable.
* [4]**Informed Consent Statement:** Not applicable.
* [5]**Data Availability Statement:** The data presented in this study are available in Supplementary Materials.
* [6]**Acknowledgments:** The authors are grateful to [PERSON] of the University of M'sila, Algeria, for plant description and identification. They also thank the Ministry of Higher Education and Scientific Research of Algeria for the financial support.
* [7]**Conflicts of Interest:** The authors declare no conflict of interest.