## 3 Results
### Screening and Analysis of CsAAAT Genes during the Post-Harvest Process of Oolong Tea  
Based on the transcriptome database of oolong tea processing samples generated by the research group [10; 37], a local BLAST operation was performed on the nucleic acid sequences of the _CsAAAT1_ (Accession No. MH544095) and _CsAAAT2_ genes (Accession No. MH544096). Nine highly similar sequences to the _CsAAAT1_ gene were screened (>99%, E_value = 0), tentatively named _CsAAAT1-1_ to _CsAAAT1-9_, whose sequence lengths ranged from 567 bp to 1422 bp. Similarly, eight highly similar sequences to _CsAAAT2_ were screened (>99%, E_value = 0), tentatively named _CsAAAT2-1_ to _CsAAAT2-8_, and the sequence lengths ranged from 1053 bp to 1572 bp (Table 1). We named these two groups the _CsAAAT1_-type genes and the _CsAAAT2_-type genes. The nucleic acid sequences of these two groups of genes are presented in Table S3. The phylogenetic tree constructed based on the sequence homology of the protein sequences of _CsAAAT1_ and _CsAAAT2_ found that two types of genes could be clustered into one cluster (Figure 5). \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Gene Name** & **Transcript** & **Number** & **Sequence Length/bp** & **Score/Site** & **E Values** & **Identity** \\ \hline \multirow{7}{*}{
\begin{tabular}{} \end{tabular} } & Unigene22082\_All & _CsAAAT1-1_ & 1422 & 2486 & 0.0 & 1263/1266 (99\%) \\  & Unigene17062\_All & _CsAAAT1-2_ & 1266 & 2478 & 0.0 & 1262/1266 (99\%) \\  & Unigene13237\_All & _CsAAAT1-3_ & 1329 & 2242 & 0.0 & 1137/1139 (99\%) \\  & Unigene65900\_All & _CsAAAT1-4_ & 1059 & 1744 & 0.0 & 886/888 (99\%) \\  & Unigene71295\_All & _CsAAAT1-5_ & 903 & 1729 & 0.0 & 884/888 (99\%) \\  & Unigene65894\_All & _CsAAAT1-6_ & 1059 & 1697 & 0.0 & 880/888 (99\%) \\  & Unigene3903\_All & _CsAAAT1-7_ & 858 & 1586 & 0.0 & 818/824 (99\%) \\  & Unigene39034\_All & _CsAAAT1-8_ & 993 & 1586 & 0.0 & 818/824 (99\%) \\  & Unigene63900\_All & _CsAAAT1-9_ & 567 & 1108 & 0.0 & 565/567 (99\%) \\  & CL11783.Contig7\_All & _CsAAAT2-1_ & 1266 & 2478 & 0.0 & 1262/1266 (99\%) \\  & CL11783.Contig1\_All & _CsAAAT2-2_ & 1572 & 2478 & 0.0 & 1262/1266 (99\%) \\ _CsAAAT2_ & CL11783.Contig12\_All & _CsAAAT2-3_ & 1227 & 2401 & 0.0 & 1223/1227 (99\%) \\  & CL11783.Contig2\_All & _CsAAAT2-4_ & 1167 & 2244 & 0.0 & 1144/1148 (99\%) \\ (1266 bp) & CL11783.Contig5\_All & _CsAAAT2-5_ & 1161 & 2060 & 0.0 & 1051/1055 (99\%) \\  & CL11783.Contig8\_All & _CsAAAT2-6_ & 1026 & 1748 & 0.0 & 894/898 (99\%) \\  & CL11783.Contig6\_All & _CsAAAT2-7_ & 1053 & 1628 & 0.0 & 833/837 (99\%) \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Local BLAST results of _CsAAAT_ genes based on a transcriptome database. The fragments per kilobase per million (FPKM) of 16 candidate _CsAAAT_ genes was analyzed using a cluster heatmap. The results showed that the expression values of the _CsAAAT_ genes could be divided into three groups: group A, group B, and group C. Group A contained seven genes. As for the FPKM in this group, the values of _CsAAAT1-1_, _CsAAAT1-3_, and _CsAAAT1-9_ in T3 were significantly higher than those in CK3 (\(p<0.01\)), while the value of _CsAAAT2-3_ in T3 was significantly higher than that in CK3 (\(p<0.05\)). There were three genes in group B, and the FPKM of this group generally decreased after the solar-withering treatment, but _CsAAAT2-5_ and _CsAAAT2-1_ were upregulated after the turnover treatment, and the difference in the FPKM between T3 and CK3 was highly significant (\(p<0.01\)). The overall expression value trends of six genes in group C were similar to those in group A, but the expression abundance was lower (Figure 6). Figure 5: Phylogenetic tree based on protein sequence homology of the _CsAAAT1_ and _CsAAAT2_ genes. Based on the transcriptome database of the oolong tea processing samples, a phylogenetic tree of the _CsAAAT1_ and _CsAAAT2_ genes, registered in GenBank, was constructed with a bootstrap value of 1000.