## 3 Results
### Aspangus Antioxidants Variation: Effect of UV-C Treatment, Storage Time, and Their Interaction
The proteins reached the highest content with UV-C2 and showed significant decreases at UV-C4 with respect to the control. No significant effects were observed for the fiber content. The value of L\({}^{*}\) in the Tips did not show significant differences with increasing doses of UV-C, while in the Middle section, it showed a decreasing tendency. The Tip color parameters a\({}^{*}\) and b\({}^{*}\) had similar behavior, and it is observed that with UV-C3 and UV-C4, both the a\({}^{*}\) and b\({}^{*}\) values remained unchanged when compared to the control; vice versa, the lower doses (UV-C1 and UV-C2) determined a significant variation. The a\({}^{*}\) value increased by 44% and 37% at UV-C1 and UV-C2, respectively. Regarding the b\({}^{*}\) value, it decreased by about 23% and 17% at UV-C1 and UV-C2, respectively. Also, in the Middle section, the values of a\({}^{*}\), b\({}^{*}\), and L\({}^{*}\) varied due \begin{table}
\begin{tabular}{l l l l l} \hline \multirow{2}{*}{**Traits**} & \multicolumn{2}{l}{**Storage Time**} & \multirow{2}{*}{**T3**} \\ \cline{2-3} \cline{5-5}  & **T0** & **T1** & **T2** & **T3** \\ \hline TPC (mg FAE/g) & 17.53 \(\pm\) 1.77 ab & 17.40 \(\pm\) 1.76 b & 18.74 \(\pm\) 2.59 ab & 18.79 \(\pm\) 2.03 a \\ TTC (mg CE/g) & 4.66 \(\pm\) 0.75 a & 4.52 \(\pm\) 0.73 ab & 4.21 \(\pm\) 0.86 ab & 3.93 \(\pm\) 0.59 b \\ ABTS (\(\mu\)u TE/g) & 20.41 \(\pm\) 1.69 b & 21.19 \(\pm\) 2.46 b & 22.80 \(\pm\) 3.46 a & 20.81 \(\pm\) 2.05 b \\ DPPH (\(\mu\)u TE/g) & 34.16 \(\pm\) 1.31 a & 34.13 \(\pm\) 1.51 a & 35.10 \(\pm\) 2.32 a & 32.15 \(\pm\) 2.09 b \\ Chlorogenic acid (ug/g) & 99.76 \(\pm\) 39.20 c & 109.38 \(\pm\) 23.90 c & 256.09 \(\pm\) 128.775 b & 345.51 \(\pm\) 115.95 a \\ Ferulic acid (ug/g) & 103.67 \(\pm\) 17.93 b & 97.27 \(\pm\) 20.50 bc & 123.28 \(\pm\) 53.14 a & 81.38 \(\pm\) 21.48 c \\ Caffeic acid (ug/g) & 396.93 \(\pm\) 50.91 a & 379.55 \(\pm\) 72.06 a & 328.62 \(\pm\) 72.23 b & 261.40 \(\pm\) 105.13 c \\ _trans_-Cinnamic acid (ug/g) & 19.66 \(\pm\) 4.22 c & 20.67 \(\pm\) 5.03 c & 49.21 \(\pm\) 8.57 b & 63.04 \(\pm\) 20.35 a \\ Rutin (ug/g) & 6477.31 \(\pm\) 524.87 a & 5878.37 \(\pm\) 375.26 b & 5030.87 \(\pm\) 1000.21 c & 4350.79 \(\pm\) 616.53 d \\ Quercetin (ug/g) & 233.50 \(\pm\) 30.13 c & 287.37 \(\pm\) 52.09 b & 362.71 \(\pm\) 25.70 a & 362.56 \(\pm\) 39.32 a \\ Kaempferol-3-O-rutinoside & 170.38 \(\pm\) 14.56 b & 153.15 \(\pm\) 18.26 c & 201.05 \(\pm\) 35.51 a & 170.39 \(\pm\) 26.53 b \\ \hline \end{tabular} Data represents the average storage time \(\pm\) SD values calculated over all UV-C. TPC = total phenolic content; TFC = total flavonoid content; ABTS = 2,2-azine-bis-[3-ethylbenzothiazazoline 6-sulphonic acid]; DPPH = 2.2\({}^{\prime}\)-diphenyl-1-picrylhydraz]. Values in the same row followed by different letters are significantly different (Tukey test \(p<\) 0.05). T0 = the first day of each UV-C treatment; T1 = 7 th day of storage; T2 = 14 th day of storage, and T3 = 21 st day of storage.  
\end{table} 
Table 2: Main effects of storage time on antioxidants. to the UV-C treatment. However, at UV-C3, it was observed that all the color parameters approached those of the control. The main effects of storage time are reported in Table 4 and Supplementary Table S1. The hardness was significantly influenced by storage, and an increase of 22% from T0 to T3 was observed. Fiber content did not show significant variations over time, unlike protein content, which increased significantly by 7% from T0 to T2 and T3. As regards the color of the spears during storage, in the Tips, an increase in L* can be seen up to T3, while a* and b* did not differ significantly from T0; in the Middle, on the contrary, a decrease in lightness was observed, compared to T0. During the storage time, variations in a* Middle and b* Middle were also observed for T0. The increase observed over time for a* was from \(-\)10.91 to \(-\)8.75, while regarding b*, it decreased from 23.32 to 19.76.