## 4 Materials and Methods
### DNA Manipulation  
Vectors for PANB and Avwf\({}^{-}\) construction were created by circular polymerase extension cloning (CPEC) protocol [40]. The vector pEMG-lapB-_lapA_ was made from the pEMG backbone, omitting the _lacZ_ DNA [28] (Table 1). Therefore, the pEMG and two fragments, the 5\({}^{\prime}\) terminus of _lapA_ and _lapB_, were amplified together. The pEMG was amplified with oligonucleotides 1.2-EMG5\({}^{\prime}\) and 3.1-lapB3\({}^{\prime}\)-SceI-EMG3\({}^{\prime}\) (Table S1). The oligonucleotides 5.1-lapB5-DS-SD-lapA5\({}^{\prime}\) and 2.3.-EMG-SceI-lapA3\({}^{\prime}\) were used to amplify the first 743 nucleotides of _lapA 5\({}^{\prime}\)_ terminus from _P. putida_ PaW85 chromosome (Table S1). The oligonucleotides 4.1-lapB3\({}^{\prime}\) and 6.1-lapB5\({}^{\prime}\) were used to amplify the first 750 nucleotides of _lapB 5\({}^{\prime}\)_ terminus from _P. putida_ PaW85 chromosome (Table S1). The resulting vector pEMG-lapB-lapA carried oppositely orientated _lapA_ and _lapB 5\({}^{\prime}\)_ terminus (Figure S1). The vector pEMG-lapB-Pm-xylS-lacI-P\({}_{lac}\)-lapA was amplified together from pEMG-lapB-lapA and two fragments, one carrying the lacI-P\({}_{lac}\) cassette and the other holding the xylS-Pm cassette (Table 1). The oligonucleotides 11-Pm-DS-lapB5\({}^{\prime}\) and 12-P\({}_{lac}\)-SD-lapA5\({}^{\prime}\) were used for pEMG-lapB-lapA amplification, 9-T1-xylS-lacI3\({}^{\prime}\) and 10-lapA5\({}^{\prime}\)-DS-P\({}_{lac}\) for _lacI-P\({}_{lac}\)_ cassette amplification from the vector pGP-FpFy-Km-lacIta-lapPSD, and 7-lapB5\({}^{\prime}\)-DS-Pm and 8-lacI3\({}^{\prime}\)-xylS-T1 for xylS-Pm cassette amplification from the vector pSEVA228S [21; 29] (Table S1). The resulting vector pEMG-lapB-Pm-xylS-lacI-P\({}_{lac}\)-lapA carried the P\({}_{lac}\) promoter in front of the 5\({}^{\prime}\) terminus of the _lapA_ gene and the Pm promoter in front of the 5\({}^{\prime}\) terminus of _lapB_ (Figure S2). The final vector pEMG-P\({}_{lac}\)-A-natB used to construct the PANB-strains was generated from the backbone of pEMG-lapB-Pm-xylS-lacI-P\({}_{lac}\)-lapA, omitting the xylS-Pm cassette (Table 1). The oligonucleotides 1.3-(lap)Aprom3\({}^{\prime}\)-T1 and 3.3-(lap)Aprom5\({}^{\prime}\)-EMG3 were used for amplification of pEMG-lapB-Pm-xylS-lacI-P\({}_{lac}\)-lapA backbone, and 13-(lap)Aprom3\({}^{\prime}\) and 14-EMG3-(lap)Aprom5\({}^{\prime}\) were used for amplification of _lapAB_ promoter-area from _P. putida_ PaW85 chromosome, 770 nucleotides from _lapA_ gene. The resulting vector pEMG-P\({}_{lac}\)-A-natB carried P\({}_{lac}\) promoter in front of _lapA 5\({}^{\prime}\)_ terminus, _lacI_ gene, and 770 nucleotides of _lapAB_ promoter-area (Figure S3). The vector pGNW-lapA-Avwf\({}^{-}\), for deletion of potential vWFa domain encoding DNA in _lapA_ gene, was made from the pGNW2 backbone, omitting the _lacZ_ DNA [30] (Table 1 and Figure S4). Therefore, the pGNW2 and two fragments, the 5\({}^{\prime}\) and 3\({}^{\prime}\) DNA from the vWFa domain of _lapA_, were amplified together. The oligonucleotides 3.5-A5 vwf5-SceI-EMG3 and 1.2-EMG5\({}^{\prime}\) were used for pGNW2 amplification (Table S1). The 883-bp-long 5\({}^{\prime}\) DNA from the potential vWFa domain was amplified with the oligonucleotides 4.5-A5 vwf5 and 6.5.1-A3 vwf5-A5 vwf3 from the _P. putida_ PaW85 chromosome (Table S1). The 806-bp-long 3\({}^{\prime}\) DNA from the vWFa domain was amplified with 5.5.1-A5 vwf3-A3 vwf5 and 2.5.-EMG-SceI-A3 vwf3 from the _P. putida_ PaW85 chromosome (Table S1). For the construction of _lapG_ (PP_0164) and _lapE_ (PP_4519) deletion mutants, the DNA regions that flanked the deleted genes were cloned into the suicide vector pEMG [28]. _P. putida_ PaW85 chromosome was used for the 470 bp long upstream DNA and 607 bp long downstream DNA of _lapG_ amplification with oligonucleotides PP0164-I-fw and PP0164-I-rev, and PP0164-2-fw and PP0164-2-rev, respectively.