## 3 Results
### The Effect of Algorithm-Predicted Fungi on Cellular Activity of Human Cell Lines  
To validate the predictive capacity of our algorithm for putative toxicity-related proteins in fungi, we selected 14 fungal species that exhibited significant protein numbers for normal, toxic, and virulent proteins according to our algorithm. For assessing the impact of these fungal species on cell viability in human cell lines MRC5 and Hela, we initially established a threshold for cytotoxic concentrations of fungal samples using serial concentrations of the _C. cladosporioides_ sample. _C. cladosporioides_ is known to cause seasonal allergic reactions but does not cause invasive infections in animals (Figure 3A). We found a discernible decrease in cell viability for both MRC5 and Hela cells at the concentration of \(1\times 10^{5}\) CFU of _C. cladosporioides_. Utilizing this concentration as the standard experimental condition for observing cell viability, we observed consistent patterns of cell viability changes across both human cell lines in response to respective fungal samples (Figure 3B). This suggests the possibility of common signaling or damaging pathways of both cell types affected by fungal components. _A. niger_, _B. Adusta_, _C. cladosporioides_, and _F. equiseti_ exhibited minimal effects on the viability of both cell lines. In contrast, the remaining 10 species caused a notable reduction, hovering around 50% in cell viability across both cell lines. This result underscores the variability in the cytotoxicity of fungal content, highlighting fungal-species-dependent responses in this in vitro evaluation test for fungi.  
\begin{table}
\begin{tabular}{l c c c c} \hline
**Fungi Species and Strand** & **Description** & **Normal** & **Toxic** & **Virulent** \\ \hline _Fusarium proliferation_ (strain ET1) & Uncharacterized protein & 0.0434 & 0.0369 & 0.9197 \\ _Alternaria alternata_ & Uncharacterized protein & 0.0433 & 0.0370 & 0.9197 \\ _Alternaria alternata_ & Uncharacterized protein & 0.0433 & 0.0370 & 0.9197 \\ _Fusarium proliferation_ (strain ET1) & Uncharacterized protein & 0.0433 & 0.0370 & 0.9197 \\ _Fusarium proliferation_ (strain ET1) & Uncharacterized protein & 0.0433 & 0.0370 & 0.9197 \\ _Fusarium proliferation_ (strain ET1) & Uncharacterized protein & 0.0433 & 0.0370 & 0.9197 \\ _Fusarium proliferation_ (strain ET1) & Uncharacterized protein & 0.0434 & 0.0369 & 0.9197 \\ _Alternaria alternata_ & Uncharacterized protein & 0.0433 & 0.0370 & 0.9197 \\ _Alternaria alternata_ & Uncharacterized protein & 0.0434 & 0.0369 & 0.9197 \\ \hline \end{tabular}
\end{table}
Table 4: Inference results for possible virulent proteins.  
\begin{table}
\begin{tabular}{l c c c} \hline
**Fungi Species and Strand** & **Description** & **Normal** & **Toxic** & **Virulent** \\ \hline _Fusarium proliferation_ (strain ET1) & Sister chromatid cohesion protein & 0.9094 & 0.0465 & 0.0441 \\ _Penicillium brasilanum_ & RNA polymerase I-specific transcription initiation factor RRN6-like protein & 0.9093 & 0.0464 & 0.0443 \\ _Alternaria alternata_ & Scaffid protein Scd2 & 0.9081 & 0.0474 & 0.0445 \\ _Alternaria alternata_ & Cysteine-rich transmembrane CYSTM domain-containing protein & 0.9081 & 0.0476 & 0.0443 \\ _Alternaria alternata_ & Protein kinase domain-containing protein & 0.9081 & 0.0471 & 0.0448 \\ _Penicillium brasilanum_ & Ribosome biogenesis protein & 0.9081 & 0.0474 & 0.0445 \\ _Alternaria alternata_ & DNA polymerase subunit delta-2 & 0.9081 & 0.0472 & 0.0447 \\ _Penicillium brasilanum_ & Quinate dehydrogenase & 0.9075 & 0.0468 & 0.0457 \\ _Fusarium proliferation_ (strain ET1) & Related to peptide transport protein & 0.9075 & 0.0470 & 0.0455 \\ _Alternaria alternata_ & HET-domain-containing protein & 0.9023 & 0.0530 & 0.0447 \\ \hline \end{tabular}
\end{table}
Table 5: Inference results for possible normal proteins.