###### Abstract  
_Agastache rugosa_, or Korean mint, is an herb used as a spice, food additive and traditional medicinal ingredient. It has desirable effects, such as its antibacterial, antifungal and antioxidant properties. _A. rugosa_ contains many phenolic compounds studied for their various health benefits, with the primary components being tilian. _A. rugosa_ extract (ARE), which was extracted with ethanol and freeze-dried, contained 21.14 \(\pm\) 0.15 mg/g of tilianin with a total polyphenol content of 38.11 \(\pm\) 0.88 mg/g. Next, the antiadipogenic effect of _A. rugosa_ and tilianin was clarified using 3T3-L1 cells, which differentiate into adipocytes and develop lipid droplets. 3T3-L1 cells were treated with ARE or tilianin and lipid accumulation (%) was calculated through oil red O staining. Tilianin elicited dose-dependent decrease in lipid accumulation (% of positive control) (00 uM 92.10 \(\pm\) 1.19%; 50 uM 69.25 \(\pm\) 1.78%;70 uM 54.86 \(\pm\) 1.76%; non-differentiation 18.10 \(\pm\) 0.32%), assessed by oil-red-O staining, whereas ARE treatments caused consistent diminution in lipid accumulation regardless of dose (100 uM 86.90 \(\pm\) 4.97%; 200 uM 87.25 \(\pm\) 4.34%; 400 uM 88.54 \(\pm\) 2.27%; non-differentiation 17.96 \(\pm\) 1.30%), indicating that both compounds have anti-obesity effects on adipocytes. Treatment with ARE lowered the mRNA (PPARy; C/EBP; c; FABP4; SREBP1; ACC; FAS) and protein (PPARy; C/EBP; SREBP1) levels of adipogenesis and lipogenesis-related factors. Tilianin showed a greater effect on the mRNA levels compared with ARE. Thus, tilianin and ARE may have anti-adipogenic and anti-lipogenic effects on 3T3-L1 cells and be possible candidates of obesity-related supplements.  
tilianin; _Agastache rugosa_; Korean mint; anti-adipogenesis; anti-lipogenesis; 3T3-L1 cells +
Footnote †: journal: Medical  
information to the nucleus to regulate the transcription of genes related to cholesterol uptake and synthesis [5]. SREBPs control transcription of enzymes involved in fatty acid uptake and biosynthesis, including acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl CoA desaturase and lipoprotein lipase. SREBPs thus coordinate the synthesis of the two main components of membranes, i.e., fatty acids and cholesterol [6; 7]. When SREBPs become overexpressed, the inhibition of adipocyte differentiation and production of a lipodystrophy occurs. In addition, the amount of white adipose tissue (WAT) is decreased and exhibits hyperglycemia and intensified fatty livers [8].  
Polyphenols in plants are known for their various biochemical properties, including antioxidant effects. Tilianin (Figure 1 is a polyphenol antioxidant, and a naturally occurring phytochemical that modulates oxidative stress-related inflammation and apoptosis [9]. Tilianin also has health-promoting properties and exhibits cardioprotective and antihypertensive behavior. [10; 11; 12; 13].  
_A. rugosa_, or Korean mint, grows throughout East Asia, especially in Korea. _A. rugosa_ is often used as a medication or gas-reducing agent in traditional medicine [14] and is known for its antifungal, anti-inflammatory, antioxidant and anti-atherogenic properties [15; 16; 17; 18; 19]. Therefore, based on the anti-atherosclerotic and anti-inflammatory activity of _A. rugosa_, we speculated the antiadipogenic predisposition of _Agastache rugosa_ extract by considering the association with these previous studies [16; 17; 18; 19].  
In present study, to elucidate the anti-obesity activity as a new function of _A. rugosa_, we investigated the adipogenesis inhibitory activity of _A. rugosa_ and its major active compound, tilianin, in murine adipocyte 3T3-L1.