## 3 Results  
### Aflatoxin Concentration in Feed, Raw Milk and Dairy Products  
Occurrence of aflat in feed, raw milk and dairy products is presented in Table 1. Based on the results of feed analyses during the period 2014-2018, the values showed that incidence of AFB1 contamination of the samples with content exceeding EU and Serbian regulation was 4.6% (highest during winter and autumn \(-\)9.0% and 5.9%, respectively). Concentration above US ML was observed in 0.4% of the samples. However, in our case, the results for feedstuffs for dairy animals indicated that there was no statistically significant association between the feed classes based on the AFB1 and seasons, Table 1 (\(\chi^{2}\) = 17.268; df = 12; \(p>0.05\)). However, multiple comparisons between seasons reveals statistically significant difference comparing samples within EU/Serbian ML and exceeding ML (\(\chi^{2}\) = 12.36; df = 3; \(p<0.05\)). Seasonal variation analysis is important due to the fact that optimal environmental conditions for _A. flavus_ are temperatures between 25 \({}^{\circ}\)C and 42 \({}^{\circ}\)C, and water activity of 0.78 under dry conditions [38]. Previous experiences showed that severe drought summers (like in 2012) caused contamination of both feed and milk product in south-east parts of Europe, like Serbia, Croatia, and North Macedonia [39]. The same study revealed correlation between low precipitation during summer and concentration of AFB1 in maize as the main feed ingredient in Serbia.  
A total of 385 raw milk samples were examined for AFM1 during the period between 2015 and 2018. Occurrence and seasonal variations are presented in Table 1. It is revealed that incidence of contamination of raw milk samples with AFM1 above the EU ML was 46.2%, with 21.3% exceeding Serbian regulation. Compared to previous studies from Serbia on AFM1 in raw milk, during 2013-2014, total samples exceeding EU ML was 56.3% [2] while in 2015 it was 44.9% [17], showing similar trends. The results also indicated that there was a statistically significant association between the raw milk classes based on the AFM1 and seasons, Table 1 (\(\chi^{2}\) = 99.686; df = 12; \(p<0.05\)). It was shown that during autumn and spring, farms delivered an inferior quality of raw milk compared to summer and winter, in terms of aflatoxin contamination. Multiple comparisons between seasons revealed statistically significant difference comparing samples within Serbian ML and exceeding ML (\(\chi^{2}\) = 55.038; df = 3; \(p<0.05\)).  
Regarding dairy products the results are promising, indicating high level of incoming control at dairy plants. As shown in Table 1, out of 556 samples tested during the period 2015-2018, only 1.3% exceeded EU ML with only 0.1% exceeding ML specified in Serbian regulation. Average values were in the range between 0.013 and 0.018 ug kg\({}^{-1}\). This showed significant improvement compared to the period 2013-2014 when over 30% of samples had values over 0.05 ug kg\({}^{-1}\)[2] and 4.2% of samples analyzed during 2015 [17]. Concerning seasonal variations, there was a statistically significant association observed between the milk classes and seasons (\(\chi^{2}\) = 34.635; df = 12; \(p<0.05\)). However, multiple comparisons between seasons revealed no statistically significant difference comparing samples within Serbian ML and exceeding ML (\(\chi^{2}\) = 1.235; df = 3; \(p>0.05\)).