## 3 Results
### 5-LOX Interactions  
The interaction parameters of the selected terpenes with 5-LOX, compared to AA, are summarized in Table 3.  
Limonene (\(-5.57\) kcal/mol), menthol (\(-5.45\) kcal/mol), and AA (\(-5.45\) kcal/mol) exhibit the lowest binding energies, indicating stable interactions with 5-LOX. The remaining terpen \begin{table}
\begin{tabular}{l c c c c c c c} \hline \hline
**5-LOX** & **Limonene** & **Camphor** & **Linalool** & **Menthol** & **\(\alpha\)-Pinene** & **\(\beta\)-Pinene** & **AA** \\ \hline Conformations & 15 & 13 & 1 & 14 & 32 & 26 & 1 \\ \hline RMSD from & & & & & & & \\ Reference & 64.854 A & 77.022 A & 23.527 A & 64.372 A & 19.328 A & 18.577 A & 28.948 A \\ Structure & & & & & & & \\ \hline Binding Energy & \(-5.57\) & \(-5.17\) & \(-4.43\) & \(-5.45\) & \(-4.89\) & \(-4.85\) & \(-5.45\) \\  & kcal/mol & kcal/mol & kcal/mol & kcal/mol & kcal/mol & kcal/mol & kcal/mol \\ \hline Inhibition & & & & & & & \\ Constant, Ki & 82.28 uM & 161.15 uM & 564.63 uM & 102.00 uM & 261.53 uM & 280.35 uM & 101.13 uM \\ \hline Total Internal & \(-0.13\) & \(+0.00\) & \(-0.39\) & \(-0.16\) & \(+0.00\) & \(+0.00\) & \(-1.08\) \\ Energy & kcal/mol & kcal/mol & kcal/mol & kcal/mol & kcal/mol & kcal/mol & kcal/mol \\ \hline Torsional Free & \(+0.30\) & \(+0.00\) & \(+1.49\) & \(+0.60\) & \(+0.00\) & \(+0.00\) & \(+4.47\) \\ Energy & kcal/mol & kcal/mol & kcal/mol & kcal/mol & kcal/mol & kcal/mol & kcal/mol \\ \hline Unbound Energy & \(-0.13\) & \(+0.00\) & \(-0.39\) & \(-0.16\) & \(+0.00\) & \(+0.00\) & \(-1.08\) \\  & kcal/mol & kcal/mol & kcal/mol & kcal/mol & kcal/mol & kcal/mol & kcal/mol \\ \hline \hline \end{tabular}
\end{table} 
Table 3: In silico docking results for interactions between selected terpen and 5-LOX: binding energy, conformational parameters, inhibition constants (Ki), and energy descriptors compared to arachidonic acid (AA). Figure 2: The comparative 3D visualization of the binding interactions between the selected terpenes, arachidonic acid (AA), and the COX-2 enzyme. also demonstrate relatively favorable binding energies, with camphor displaying the highest value (\(-5.17\) kcal/mol), which may suggest weaker interaction with the enzyme. Limonene (82.28 uM), AA (101.13 uM), and menthol (102.00 uM) exhibit the lowest Ki values among the tested compounds, identifying them as the most promising potential 5-LOX inhibitors. In contrast, linalool shows a substantially higher Ki value (564.63 uM), indicating a significantly lower affinity for 5-LOX. High RMSD values (>50 A) are observed for camphor (77.022 A) and limonene (64.854 A), suggesting notable structural changes upon binding. Conversely, \(\alpha\)-pinene (19.328 A) and \(\beta\)-pinene (18.577 A) demonstrate the lowest RMSD values, indicating minimal conformational changes during interaction with the enzyme. Linalool (+1.49 kcal/mol) and AA (+4.47 kcal/mol) exhibit the highest torsional energies, suggesting greater conformational flexibility. In contrast, menthol (+0.60 kcal/mol) and \(\alpha\)-pinene (+0.00 kcal/mol) show the lowest torsional energy values, indicating stable conformations upon binding to 5-LOX. The lowest unbound energy is recorded for AA (\(-1.08\) kcal/mol), supporting its high affinity for the enzyme. The analysis reveals that limonene, menthol, and AA display the lowest binding energies, suggesting stable interactions with 5-LOX. Menthol and AA also demonstrate the lowest Ki values. In contrast, linalool exhibits the weakest affinity, suggesting lower inhibitory potential. A 3D visualization of the interactions between the terpen, AA, and the 5-LOX enzyme is presented in Figure 3. Ligands are shown in the ball-and-stick representation (light brown), and the enzyme is displayed as a cartoon structure (cyan). Hydrogen bonds and relevant interactions are indicated with dashed lines where applicable. The positioning of each ligand relative to key catalytic residues illustrates potential binding modes and differences in spatial orientation.