## 3 Results
### Proteolytic Modification in Wheat Malts from Commodity Field Experiment  
Protein transformations occurring during malting in wheat grain significantly affect the quality of the malt obtained from it. The content of total protein in the wheat malt obtained from wheat grain of the Elixer cultivar in the first year of the field study in farms B and E (Table 7) was above the norm, measuring 12.3-12.6% d. m., while the other values were within the norm. The soluble protein content averaged 3.77% d. m., and the highest value was obtained in the raw material from farm E. The Kolbach number in the years of this study was 32.8%, and the lowest value was obtained in the first study year in farm B (28.5%). The average content of free amine nitrogen in the years of the study was 106.91 mg\(\cdot\)L\({}^{-1}\), and the highest value was obtained for wheat malt produced from winter wheat grains in the first study year in farm G. Comparing the average content of total protein, soluble protein, free amino nitrogen (FAN), and Kolbach number for malts obtained from winter wheat grains of the Elixer cultivar in field and canopy experiments, it was observed that the content of total protein in canopy experiments was 13.79% higher, while the contents of soluble protein and FAN were lower by 11.50% and 9.31%, respectively. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Growing Season** & **Cultivar** & **Control** & **Variant I** & **Variant II** & **Variant III** \\ \hline \multirow{4}{*}{2020/21} & Elixer & 113.95 BCa & 119.99 Cb & 126.29 Fc & 126.66 DEc \\ \cline{2-6}  & Lawina & 104.80 Aa & 106.31 Aab & 109.87 ABb & 109.69 Ab \\ \cline{2-6}  & Gimantis & 115.98 Cab & 113.41 Ba & 119.25 Db & 128.03 Ec \\ \cline{2-6}  & Rockefeller & 103.65 Aa & 105.65 Aa & 106.70 Aa & 109.60 Ab \\ \hline \multirow{4}{*}{2021/22} & Elixer & 108.08 ABa & 115.79 BCb & 122.98 Ec & 122.42 Cc \\ \cline{2-6}  & Lawina & 103.81 Aa & 111.99 ABb & 117.20 CDc & 115.04 Bbc \\ \cline{2-6}  & Gimantis & 109.94 BCA & 116.00 BCb & 122.97 Ec & 127.13 Ed \\ \cline{2-6}  & Rockefeller & 108.09 ABa & 107.16 Aa & 115.79 Cb & 115.38 Bb \\ \hline \multirow{4}{*}{2022/23} & Elixer & 111.78 Ba & 116.12 BCb & 113.94 BCab & 116.73 Bb \\ \cline{2-6}  & Lawina & 108.12 Aa & 114.78 Bb & 118.66 Db & 117.15 Bb \\ \cline{2-6}  & Gimantis & 111.11 Ba & 117.07 Cb & 127.78 Fc & 124.76 CDc \\ \cline{2-6}  & Rockefeller & 104.52 Aa & 107.09 Aab & 109.03 ABb & 107.94 Aab \\ \hline \multirow{4}{*}{Yearly Average} & & 2020/21 & 113.74 1 \(\pm\) 8.18 \\ \cline{2-6}  & & 2021/22 & 114.99 1 \(\pm\) 6.60 \\ \cline{2-6}  & & 2022/23 & 114.16 1 \(\pm\) 6.35 \\ \hline \multirow{4}{*}{Cultivar Average} & & Elixer & 117.89 8 \(\pm\) 5.81 \\ \cline{2-6}  & Lawina & 111.45 \(\mathrm{{}^{A}}\)\(\pm\) 5.11 \\ \cline{2-6}  & Gimantis & 119.45 \(\mathrm{{}^{B}}\)\(\pm\) 6.53 \\ \cline{2-6}  & Rockefeller & 108.38 \(\mathrm{{}^{A}}\)\(\pm\) 3.78 \\ \hline \multirow{4}{*}{Fertilization Average} & Control & 108.65 \(\mathrm{{}^{a}}\)\(\pm\) 4.05 \\ \cline{2-6}  & Variant I & 112.61 \(\mathrm{{}^{ab}}\)\(\pm\) 4.88 \\ \cline{2-6}  & Variant II & 117.54 \(\mathrm{{}^{b}}\)\(\pm\) 6.81 \\ \cline{2-6}  & Variant III & 118.38 \(\mathrm{{}^{b}}\)\(\pm\) 7.27 \\ \hline \multicolumn{2}{c}{**Average**} & \multicolumn{2}{c}{**114.30 \(\pm\) 3.81**} \\ \hline \hline \end{tabular} Data are expressed as a mean value (n = 3) \(\pm\) SD, with SD as the standard deviation. Mean values within a rows with different letters are significantly different (A–E, statistical differences between cultivars; and a–d, statistical differences at the analyzed levels of nitrogen fertilization, 1, statistical differences at the growing season with \(p<0.05\)).  
\end{table} 
Table 6: The content of free amine nitrogen (mg\(\cdot\)L\({}^{-1}\)) in wheat malts from wheat grain from field experiments in the study years analyzed. The average Kolbach number was 23.72% lower compared to the same parameters in field experiments (Tables 3-7).