## 4 Discussion and Conclusions  
Increasing levels of artificial EMF as one of the environmental physical factors should be taken into consideration in various areas related to public health or environmental safety, and increasing the knowledge base regarding the mechanisms of EMF action on biological structures is a fundamental step towards safety and sustainability. After studying the state-of-the-art research papers, we decided to perform our own experiments, discuss partial results in national and international conferences, and optimize the exposure setup in accordance with newly gained knowledge of mechanisms explaining how ELF-EMF can interact with cell organisms. Implementing sensorics monitoring technologies within the research methodology and processes, to cover all aspects that could possibly affect consistency, repeatability and reliability of results, was of utmost importance to address the diverse opinions of the scientific community related to quantum physics, cell biology, chemistry and bioelectromagnetism. Based on gained experience and experimental evidence [38; 44; 45; 46; 47; 48; 49; 50; 51; 52; 53; 54; 55], confirmed also by this study, it could be concluded, that statistically significant responses of biological system exposure to ELF MF could be observed even under conditions that do not meet the IPR requirements, as stated by the first set of experiments with the nonspecific conditions. As the IPR model elucidates how exposure to MFs can induce cellular stress (e.g., stress gene upregulation), metabolic changes (e.g., alterations in fermentation), alterations in cell division and reproduction, genetic regulation (gene expression alteration), and cumulative effects over time, as presented in Figure 10, maintaining uniformity in the static MF within the coil and ensuring it remained perpendicular to the time-varying electromagnetic field was of utmost importance. Because of that, a second, modified set of experiments was performed, with the artificial settings of IPR conditions applied. However, the main contributions of this study are not only in its extension of experimental evidence in the context of ELF MF impact on biological systems at the cellular level, but also in its MF measurements within the exposure setup. The performed measurements revealed the difficulties related to precise fulfillment of the physical conditions of the IPR mechanism even within the laboratory environment, putting into question its practical utilization as the mechanism that can explain the increasing evidence [5;  
When the IPR laboratory conditions are artificially modulated, the significance level between mean values of the control and exposed samples is even higher, at the level of 1%. Here, the mean value of C was 7.436, and the mean value of E was 9.1046. The effect size was confirmed by [PERSON]'s d and [PERSON]'s delta numbers. This higher significance supports the importance of ensuring that the conditions related to the theoretical background are met. However, we understand that non-uniformity of the background MF within the incubators could potentially introduce variability and hinder the reproducibility of studies conducted on cell structures. Notwithstanding the challenges faced, this study successfully established the statistical significance of the grid frequency EMF's impact on biological structures following a 7-h exposure. Emphasizing the imperative nature of comprehending the mechanisms underlying this effect is of utmost importance, given the ever-growing prevalence of EMFs in our everyday existence. This underscores the vital role of experimental design and stringent measurement conditions in the precise evaluation of these fields' effects. Such efforts are essential in shaping regulatory frameworks and guidelines aimed at minimizing potential harm associated with EMF exposure. Overall, the findings underscore the critical significance of precisely measuring the static component of the magnetic flux density vector in research focused on non-thermal mechanisms, such as the IPR model.