## 2 Materials and Methods  
### Formulations and Feed Manufacture  
Dietary treatments are presented in Table 1. A fish meal-based diet (45%) was used as a control treatment, whereas the experimental diets contained 29% of fishmeal and 30% of SBM from three distinct genotypes of similar genetic background and matched as closely as possible for protein content: standard soybean meal (STD SBM); a soybean genotype homozygous for the gy4 allele conditioning null 11 sA4 and 11 sA5 globulins of Glycinin, homozygous for the 1 \(\times\) 1, 1 \(\times\) 2 and 1 \(\times\) 3 alleles conditioning absence of seed lipoxygenases and homozygous for the rs2 allele conditioning near absence of seed raffinose and stachyose (TLP SBM); and a soybean genotype homozygous for the gy4 allele conditioning null 11 sA4 and 11 sA5 globulins of Glycinin (11 sA4 null SBM). All macro ingredients were milled to <750 um, and well mixed with the remaining dry ingredients, and then extruded through a Baker-Perkins MPV24 twin-screw extruder. Each feed was manufactured using a 1.5 mm \(\O\) die (\(\sim\)2.5 mm \(\O\) pellets) using standard CSIRO Extrusion protocols (Table 2). The pellets were dried at 60 \({}^{\circ}\)C for 12 h, after which they were vacuum infused with their specific allocation of oil. All feeds were kept in frozen storage (\(-\)20 \({}^{\circ}\)C) throughout the feeding trial. All uneaten feed was removed from the collection tank of all treatments 1 h following feeding, and the collected waste feed was then dried to allow calculation of apparent feed intake and feed conversion ratio. \begin{table}
\begin{tabular}{l c c c c} \hline \hline \multicolumn{1}{c}{**Ingredients g kg\({}^{-1}\)**} & \multicolumn{1}{c}{**FM**} & \multicolumn{1}{c}{**STD**} & \multicolumn{1}{c}{**TLP**} & **11 sA4 Null** \\  & **Control** & **SBM** & **SBM** & **SBM** & **SBM** \\ \hline Fishmeal \({}^{\text{a}}\) & 450.0 & 290.0 & 290.0 & 290.0 \\ Wheat flour & 223.0 & 110.0 & 93.0 & 106.0 \\ Wheat gluten & 120.0 & 89.0 & 106.0 & 93.0 \\ Blood meal \({}^{\text{a}}\) & 60.0 & 60.0 & 60.0 & 60.0 \\ Fish oil \({}^{\text{a}}\) & 70.0 & 70.0 & 70.0 & 70.0 \\ Poultry oil \({}^{\text{a}}\) & 70.0 & 70.0 & 70.0 & 70.0 \\ Stay-C 35\% \({}^{\text{b}}\) & 1.0 & 1.0 & 1.0 & 1.0 \\ Vitamin mineral premix \({}^{\text{c}}\) & 6.0 & 6.0 & 6.0 & 6.0 \\ Standard soybean meal (STD SBM) \({}^{\text{d}}\) & 0.0 & 300.0 & 0.0 & 0.0 \\ Soybean meal triple lipoxygenase plus (TLP & 0.0 & 0.0 & 300.0 & 0.0 \\ SBM) \({}^{\text{d}}\) & 0.0 & 0.0 & 0.0 & 300.0 \\ Soybean meal 11 sA4 null (11 sA4 null SBM) \({}^{\text{d}}\) & 0.0 & 3.0 & 3.0 & 300.0 \\ Methionine \({}^{\text{e}}\) & 0.0 & 1.0 & 1.0 & 1.0 \\ Taurine \({}^{\text{f}}\) & 0.0 & 1.0 & 1.0 & 1.0 \\ \hline Proximate composition (g kg\({}^{-1}\)) & & & & \\ \hline Dry matter & 959 & 960 & 954 & 954 \\ Protein & 506 & 492 & 494 & 505 \\ Lipid & 166 & 171 & 179 & 170 \\ Ash & 68 & 63 & 62 & 62 \\ Gross energy (kJ g\({}^{-1}\)) & 23.7 & 24.2 & 24.3 & 24.3 \\ \hline Amino acid (g kg\({}^{-1}\)) & & & & \\ \hline ASP & 39 & 43 & 45 & 43 \\ SER & 20 & 22 & 23 & 21 \\ GLU & 88 & 87 & 93 & 89 \\ GLY & 26 & 24 & 26 & 23 \\ HIS & 16 & 15 & 15 & 15 \\ ARG & 22 & 25 & 25 & 26 \\ THR & 19 & 19 & 20 & 19 \\ ALA & 25 & 24 & 24 & 24 \\ PRO & 29 & 28 & 29 & 28 \\ CYS & 5 & 5 & 5 & 5 \\ TYR & 14 & 19 & 20 & 19 \\ VAL & 23 & 23 & 24 & 23 \\ MET & 11 & 11 & 11 & 10 \\ LYS & 26 & 28 & 28 & 28 \\ ILE & 17 & 18 & 19 & 18 \\ LEU & 38 & 38 & 39 & 38 \\ PHE & 22 & 23 & 25 & 23 \\ TAU & 3 & 3 & 3 & 3 \\ \hline \hline \end{tabular}  
* Ridley, Aquafeeds, Queensland, Australia. \({}^{\text{b}}\) DSM, Heerlen, Netherlands. \({}^{\text{c}}\) Rabar Pty Ltd., Queensland, Australia. \({}^{\text{d}}\) CSIRO, Australia. \({}^{\text{e}}\) Redox, Queensland, Australia. \({}^{\text{f}}\) Bulk Nutrients, Tasmania, Australia.  
\end{table} 
Table 1: Dietary formulation and proximate composition.