## 3 Results
The Kruskal-Wallis test showed no statistically significant differences between the treatments nor with the wild biomass for the moisture and the ash content. As to the ash content in the fresh biomass the analysis revealed a test statistic H(10) = 19.840, with a \(p\)-value of 0.031, indicating a significant difference between the groups. The highest value was recorded in the wild sample (9.08 \(\pm\) 0.18% fw) and the lowest value was registered at the end of the two weeks of cultivation with PES (7.57 \(\pm\) 0.01% fw). The organic matter was also statistically analyzed, validating the normality and homogeneity of the samples. Therefore, the univariate ANOVA conducted showed significant differences between the samples (F(10,15) = 3.576, \(p\)-value = 0.013). The seaweed cultivated after 7 days with NB had a significantly higher concentration of organic matter (22.86 \(\pm\) 0.79% fw) when compared to all the other samples, while both samples cultivated with PES and red LED light (after the first week: 18.74 \(\pm\) 0.77 and after the second week: 17.78 \(\pm\) 0.07% fw) showed a significantly lower concentration of organic matter compared to all the other samples. Regarding the dry weight, the Kruskal-Wallis test did not deliver any differences between the samples, which varied from 27.01% to 31.21% (Table 2). As to the organic matter and the ash content in the dry biomass the Kruskal-Wallis test showed significant differences between the samples. Nevertheless, some differences were noticed in dry weight for organic matter and ash (H(10) = 19.128, \(p\)-value = 0.091). For both analyses, on the seventh day of cultivation, the red LED light and the wild biomass were significantly different from those cultivated with VSE, PES, and NB. At the end of the trial, however, only the biomass cultivated with PES was significantly different, achieving the highest organic matter (73.33%) and the lowest ash content (26.67%). The CIELAB colour space consists of several components. The \(\Delta\)E values show a noticeable colour difference over time, with increasing values (Figure 5a). The Kruskal-Wallis test delivered statistically significant differences between PES and NB (H(4) = 14.809, \(p\)-value = 0.005), with PES presenting the highest colour variation (5.51 \(\pm\) 2.25) and NB the lowest (2.22 \(\pm\) 0.60). The results indicate that all treatments show a slight darkening (decrease in L*), with values decreasing slightly over time, with no statistical differences (Figure 5b). A shift towards green (decrease in a*) can be noticed, except for the blue LED \begin{table}
\begin{tabular}{c c c c c c c c c} \hline  & \multicolumn{3}{c}{**Dry Weight**} & \multicolumn{3}{c}{**Organic Matter**} & \multicolumn{3}{c}{**Ash**} \\ \hline VSE, day7 & 27.01 & \(\pm\) & 0.82 & 73.00 & \(\pm\) & 2.68 \({}^{\text{a}}\) & 27.00 & \(\pm\) & 2.68 \({}^{\text{b}}\) \\ VSE, day 14 & 29.00 & \(\pm\) & 0.56 & 71.31 & \(\pm\) & 1.38 & 28.69 & \(\pm\) & 1.38 \\ PES, day 7 & 28.88 & \(\pm\) & 1.62 & 72.52 & \(\pm\) & 0.92 \({}^{\text{a}}\) & 27.48 & \(\pm\) & 0.92 \({}^{\text{b}}\) \\ PES, day 14 & 28.39 & \(\pm\) & 1.05 & 73.33 & \(\pm\) & 1.04 \({}^{\text{a}}\) & 26.67 & \(\pm\) & 1.04 \({}^{\text{b}}\) \\ NB, day7 & 31.21 & \(\pm\) & 1.01 & 73.26 & \(\pm\) & 0.16 \({}^{\text{a}}\) & 26.74 & \(\pm\) & 0.16 \({}^{\text{b}}\) \\ NB, day 14 & 29.14 & \(\pm\) & 0.86 & 71.24 & \(\pm\) & 0.78 & 28.76 & \(\pm\) & 0.78 \\ Blue LED, day7 & 27.43 & \(\pm\) & 0.95 & 71.69 & \(\pm\) & 0.06 & 28.31 & \(\pm\) & 0.06 \\ Blue LED, day 14 & 28.06 & \(\pm\) & 0.63 & 71.24 & \(\pm\) & 0.22 & 28.76 & \(\pm\) & 0.22 \\ Red LED, day7 & 26.35 & \(\pm\) & 0.99 & 71.12 & \(\pm\) & 0.24 & 28.88 & \(\pm\) & 0.24 \\ Red LED, day 14 & 26.12 & \(\pm\) & 0.01 & 68.06 & \(\pm\) & 0.29 \({}^{\text{b}}\) & 31.94 & \(\pm\) & 0.29 \({}^{\text{a}}\) \\ Wild & 29.47 & \(\pm\) & 0.20 & 69.20 & \(\pm\) & 0.86 \({}^{\text{b}}\) & 30.80 & \(\pm\) & 0.86 \({}^{\text{a}}\) \\ \hline \end{tabular}
\end{table}