## 4 Conclusions  
The study extracted, purified and hydrolyzed protein from 'Senorita' banana pulp with the major protein having an approximate molecular weight of 15 kDa. The low concentration of the crude extract (167.32 \(\upmu\)g/mL buffer) was associated to the characteristic low protein content of the sample. Furthermore, these extracts and the peptides released during the enzymatic hydrolysis were tested for ACE inhibitory and antioxidant activities. The undigested crude sample had the highest ACE inhibitory activity (85.20%), but it was found to be significantly different from the activity of the standard, captopril (99.72%). On the other hand, the 3 h and 4 h crude digests had the highest DPPH radical scavenging activity (30.83% and 34.74%, respectively) that were found to be not significantly different to the activity of the standard, ascorbic acid (36.31%). For the effect of digestion period, longer digestion time results to higher ACE inhibitory activity while for the DPPH radical scavenging activity, there is an initial increase followed by a decline at longer digestion time. These are expected based on the result of in silico analysis of the putative major protein, lectin, and the available literatures similar to the study. Over-all, this study can serve as an additional reference to the very limited banana protein and peptide studies.  
The Supplementary Materials are available online at [[https://www.mdpi.com/2076-3417/11/5/2190/s1](https://www.mdpi.com/2076-3417/11/5/2190/s1)]([https://www.mdpi.com/2076-3417/11/5/2190/s1](https://www.mdpi.com/2076-3417/11/5/2190/s1)).  
Conceptualization, J.M.F., M.A.T., M.C.M.C. and A.H.-F.; formal analysis, J.M.F.; investigation: J.M.F.; methodology, J.M.F., M.A.T., M.C.M.C. and A.H.-F.; resources, M.A.T.; supervision, M.A.T.; visualization, J.M.F. and M.A.T.; writing--original draft preparation, J.M.F.,  
\begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Digestion Hours** & \multicolumn{4}{c}{**DPPH Radical Scavenging Activity (\%)**} \\ \cline{2-5}  & **Ascorbic Acid** & **Crude** & **Partially Purified** & **Purified** \\ \hline
0 & 36.31 \(\pm\) 0.21  & 17.21 \(\pm\) 0.27  & 18.00 \(\pm\) 0.72  & 17.37 \(\pm\) 1.69  \\
3 & \(-\) & 30.83 \(\pm\) 1.36  & 14.55 \(\pm\)1.24  & 16.59 \(\pm\) 1.65  \\
4 & \(-\) & 34.74 \(\pm\) 1.24  & 13.30 \(\pm\) 0.98  & 14.87 \(\pm\) 1.18  & 12.52 \(\pm\) 1.18  & 15.02 \(\pm\) 0.47  & 6.73 \(\pm\) 0.54  \\
24 & \(-\) & \(-\) & 107.36 \(\pm\) 5.15  & 1.41 \(\pm\) 1.69  & 1.41 \(\pm\) 2.93  \\ \hline \hline \end{tabular}
\end{table}
Table 3: DPPH radical scavenging activity (%) of crude, partially purified and purified protein extracts at different digestion hours 1.  
M.A.T. and L.Y.U.; writing--review and editing, J.M.F., M.A.T., M.C.M.C., A.H.-F. and L.Y.U. All authors have read and agreed to the published version of the manuscript.  
This research received no external funding.  
The authors acknowledge Process Biochemistry Laboratory (PS B-307)--University of the Philippines Los Banos and nearby laboratories of the Institute of Chemistry for the support on this study. The same goes with the Enhanced Creative Work and Research Grant (ECWRG), Office of the Vice President for Academic Affairs (OVPAA) of the UP System for extending their support to make this research possible.  
The authors declare no conflict of interest.