## 3 Results
### Analysis of Plants Obtained Through Apomixis Using the \"Single-Flower\" Procedure  
We further applied the developed \"single-flower\" procedure to study the variation in metabolites in the flowers at stage 4 in 19 plants grown from seeds derived from the apomitic line \(H\). _perforatum_ Hp93. The apomixis origin of the plants was proven by analysis with 10 SSR markers, which resulted in uniform SSR profiles. Individual extractions from four flowers at stage 4 were performed for each plant using the developed procedure. A summary of the obtained results is presented in Table 4. As can be seen from Table 4, eleven of the identified compounds showed low variation among the samples with RSD values below 15%, including chlorogenic acid, catechin, procyanidin B2, epicatechin, procyanidin C1, hyperoside, querciturone + isoquercitrin, quercitrin, pseudohypericin, hyperorin, and hypericin. The highest RSD value was observed for protopseudohypericin (compound **13**) and protohypericin (compound **15**), with values of 37.29% and 45.01%, respectively, followed by I3 II8 biapigenin (compound **12**) and rutin (compound **7**), with RSD values of 27.52% and 15.33%. Table 4 also shows the data for the percentage based on the combined peak areas of protopseudohypericin and pseudohypericin (compounds **13+14**) as well as protohypericin and hypericin (compounds **15+18**), which results in a significant reduction of the observed variation for these compounds based on the calculated RSD values.  
\begin{table}
\begin{tabular}{c c c c c c c} \hline  & \multicolumn{2}{c}{**3\_29**} & \multicolumn{2}{c}{**90\_44**} & \multicolumn{2}{c}{**139\_28**} & \multicolumn{2}{c}{**Mean RSD (\%)**} \\ \hline
**Compound** & **Mean (\%)** & **RSD** & **Mean (\%)** & **RSD** & **Mean (\%)** & **RSD** & \\ \hline
**1** & 3.18 \(\pm\) 0.40 b  & 12.70 & 1.82 \(\pm\) 0.2 a  & 11.03 & 2.08 \(\pm\) 0.08 a  & 3.71 & 9.15 \(\pm\) 4.78 \\
**3** & 4.15 \(\pm\) 0.28 a  & 6.83 & 1.45 \(\pm\) 0.08 b  & 5.53 & 2.81 \(\pm\) 0.19 c  & 6.86 & 6.41 \(\pm\) 0.76 \\
**4** & 1.16 \(\pm\) 0.014 a  & 12.10 & 1.42 \(\pm\) 0.04 b  & 3.15 & 1.17 \(\pm\) 0.06 a  & 4.95 & 6.73 \(\pm\) 4.73 \\
**5** & 3.88 \(\pm\) 0.35 a  & 3.09 & 5.34 \(\pm\) 0.3 b  & 5.69 & 4.51 \(\pm\) 0.23 c  & 5.01 & 4.60 \(\pm\) 1.35 \\
**6** & 1.04 \(\pm\) 0.03 a  & 3.13 & 0.82 \(\pm\) 0.01 b  & 0.90 & 0.93 \(\pm\) 0.05 c  & 5.75 & 3.26 \(\pm\) 2.43 \\
**7** & 13.37 \(\pm\) 0.90 a  & 6.73 & 0.08 \(\pm\) 0.07 b  & 7.83 & 4.78 \(\pm\) 0.38 c  & 7.99 & 7.52 \(\pm\) 0.69 \\
**8** & 13.24 \(\pm\) 0.75 a  & 5.67 & 21.14 \(\pm\) 1.73 b  & 8.25 & 14.54 \(\pm\) 0.85 a  & 5.82 & 6.58 \(\pm\) 1.45 \\
**9+10** & 8.26 \(\pm\) 0.91 a  & 11.03 & 14.80 \(\pm\) 0.68 b  & 4.62 & 10.52 \(\pm\) 0.85 c  & 8.05 & 7.90 \(\pm\) 3.21 \\
**11** & 5.41 \(\pm\) 0.58 a  & 10.69 & 5.90 \(\pm\) 0.44 a  & 7.62 & 5.55 \(\pm\) 0.65 a  & 11.72 & 10.01 \(\pm\) 2.13 \\
**12** & 11.26 \(\pm\) 1.74 b  & 15.45 & 7.78\(\pm\) 0.67 a  & 8.62 & 9.68 \(\pm\) 1.48 ab  & 15.27 & 13.11 \(\pm\) 3.89 \\
**13** & 0.97 \(\pm\) 0.10 a  & 10.37 & 0.56 \(\pm\) 0.13 a  & 22.97 & 0.86 \(\pm\) 0.3 a  & 34.92 & 22.75 \(\pm\) 12.28 \\
**14** & 6.60 \(\pm\) 0.71 a  & 10.81 & 8.17 \(\pm\) 0.89 a  & 10.98 & 7.38 \(\pm\) 0.66 a  & 8.94 & 10.24 \(\pm\) 1.13 \\
**15** & 0.41 \(\pm\) 0.05 ab  & 11.34 & 0.55 \(\pm\)0.15 b  & 27.06 & 0.29 \(\pm\) 0.1 a  & 36.51 & 24.97 \(\pm\) 12.71 \\
**17** & 23.38 \(\pm\) 3.04 a  & 12.99 & 21.94 \(\pm\) 2.94 a  & 13.53 & 30.93 \(\pm\) 2.42 b  & 7.83 & 11.45 \(\pm\) 3.15 \\
**18** & 3.68 \(\pm\) 0.50 a  & 13.65 & 8.21 \(\pm\) 0.94 b  & 11.53 & 3.98 \(\pm\) 0.25 a  & 6.17 & 10.45 \(\pm\) 3.86 \\ \hline Mean RSD & & & & & & \\ per plant & & 9.77 \(\pm\) 3.79 & & 9.95 \(\pm\) 7.01 & & 11.30 \(\pm\) 10.33 & 10.34 \(\pm\) 6.08 \\
**13+14** * & 7.58 \(\pm\) 0.81 a  & 10.74 & 8.74 \(\pm\) 0.86 a  & 9.80 & 7.38 \(\pm\) 0.66 a [FOOTNOTE:a]Footnote a: foot