## 3 Results
### Effect of the CFS on Amino Acid Content of Pacific White Shrimp  
The content of 15 amino acidsthat were changed obviously of all samples stored in various cold chain logistics are depicted in Table 6. During transportation, retailing, and domestic storage, the content of total amino acid decreased by levels of 14.96%, 24.30% and 26.23% in group CK1, CK2 and CK3, and the content of total amino acid reduced by levels of 3.09%, 7.57% and 11.82% in group SA1, SA2 and SA3 treated by CFS from _A. sobria_, respectively. In detail, compared with group CK3, group SA3 treated with CFS from _A. sobria_ inhibited the decreasing of lysine and arginine.  
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multirow{2}{*}{**Time (days)**} & \multicolumn{6}{c}{**The Content of Putrescine**} \\ \cline{2-7}  & **CK1** & **SA1** & **CK2** & **SA2** & **CK3** & **SA3** \\ \hline
9 & 212.72 \(\pm\) 0.23 \({}^{\mathrm{c}}\) & 173.04 \(\pm\) 0.32 \({}^{\mathrm{d,e}}\) & 238.19 \(\pm\) 0.13 \({}^{\mathrm{b}}\) & 194.81 \(\pm\) 1.09 \({}^{\mathrm{d}}\) & 250.56 \(\pm\) 0.31 \({}^{\mathrm{a}}\) & 210.34 \(\pm\) 0.97 \({}^{\mathrm{c}}\) \\ \hline \hline \end{tabular} Note: 1. Different letters (a–e) at each column denote significant differences (_p_ < 0.05) between means according to [PERSON]’s multiple range test.  
\end{table}
Table 4: Effect of CFS from _A. sobria_ on putrescine production of Pacific white shrimp (_L. vannamei_) during different cold chain logistics.  
Figure 5: Effect of CFS from _A. sobria_ on (**a**) putrescine and (**b**) cadaverine production of Pacific white shrimp (_L. vannamei_) during different cold chain logistics.  
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Time (days)**} & \multicolumn{6}{c}{**The Content of Cadaverine**} \\ \cline{2-7}  & **CK1** & **SA1** & **CK2** & **SA2** & **CK3** & **SA3** \\ \hline
6 & 29.43 \(\pm\) 1.92 \({}^{\mathrm{c}}\) & 25.12 \(\pm\) 3.01 \({}^{\mathrm{d}}\) & 35.12 \(\pm\) 1.21 \({}^{\mathrm{c}}\) & 29.81 \(\pm\) 0.23 \({}^{\mathrm{c}}\) & 55.23 \(\pm\) 3.70 \({}^{\mathrm{a}}\) & 40.45 \(\pm\) 2.32 \({}^{\mathrm{b}}\) \\
9 & 58.05 \(\pm\) 2.23 \({}^{\mathrm{b}}\) & 44.81 \(\pm\) 2.1 \({}^{\mathrm{c}}\) & 60.91 \(\pm\) 1.56 \({}^{\mathrm{a,b}}\) & 47.02 \(\pm\) 3.89 \({}^{\mathrm{c}}\) & 64.45 \(\pm\) 3.34 \({}^{\mathrm{a}}\) & 52.23 \(\pm\) 2.44 \({}^{\mathrm{b,c}}\) \\ \hline \hline \end{tabular} Note: 1. Different letters at each column denote significant differences (_p_ < 0.05) between means according to [PERSON]’s multiple range test.  
\end{table}
Table 5: Effect of CFS from _A. sobria_ on cadaverine production of Pacific white shrimp (_L. vannamei_) during different cold chain logistics.  
Effect of the CFS on the Sodium Dodecyl Sulfate-polyacrylamideamide Gel Electrophoresis (SDS-PAGE) Profile of the Myofibrillar Proteins of Pacific White Shrimp  
Figure 6 shows the SDS-PAGE profile of the myofibrillar proteins of shrimp during the cold chain logistics, including transportation, retailing, and domestic storage. In fresh shrimps, the bands of group SA treated by CFS from _A. sobria_ were more intense compared with control samples. Additionally, there was almost no difference in band I among group SA1, SA2 and SA3, but the band II in group SA2 was little lighter than SA1 and SA3.