## 4 Conclusions and Future Perspectives
The following supporting information can be downloaded at: [[https://www.mdpi.com/article/10.3390/app15073837/s1](https://www.mdpi.com/article/10.3390/app15073837/s1)]([https://www.mdpi.com/article/10.3390/app15073837/s1](https://www.mdpi.com/article/10.3390/app15073837/s1)), Figure S1: Absorbance spectrum of CRANAD-2 in MeOH at different concentrations; Figure S2: The transmission spectrum of the optical filter FGL695, showing the rate of transmission between range of wavelength of interest; Figure S3: Fluorescence intensity spectrum (c.p.s) comparison of CRANAD-2 (25 \(\upmu\)M) in macroscale (dark green) and microscale (light green) environments; Figure S4: Linear relationship between fibrillar A\(\beta\) protein concentrations and fluorescence intensities of CRANAD-2 (25 \(\upmu\)M), measured at peak wavelength of 736 nm: (**a**) macroscale experiments with concentrations ranging from 100 nM to 1000 nM; (**b**) microscale experiments with concentrations ranging from 100 nM to 1000 nM; (**c**) microscale experiments with concentrations ranging from 5 nM to 100 nM; Algorithm S1: Peak identification; Algorithm S2: Noise averaging. Conceptualization, R.O.R., G.M. and M.O.C. ; methodology, R.O.R. ; software, M.O.C. ; validation, R.O.R., G.M. and P.S. ; investigation, M.O.C. ; writing--original draft preparation, M.O.C. ; writing--review and editing, R.O.R., G.M. and P.S. ; supervision, R.O.R. and G.M. ; project administration, R.O.R. and G.M. ; funding acquisition, R.O.R. and G.M. All authors have read and agreed to the published version of the manuscript. This work was supported by the project PTDC/EEI-EEE/2846/2021 ([[https://doi.org/10.54499/PTDC/EEI-EEE/2846/2021](https://doi.org/10.54499/PTDC/EEI-EEE/2846/2021)]([https://doi.org/10.54499/PTDC/EEI-EEE/2846/2021](https://doi.org/10.54499/PTDC/EEI-EEE/2846/2021))(accessed on 20 March 2025)) and partially by 2022.02165.PTDC ([[https://doi.org/10.54499/2022.02165.PTDC](https://doi.org/10.54499/2022.02165.PTDC)]([https://doi.org/10.54499/2022.02165.PTDC](https://doi.org/10.54499/2022.02165.PTDC)) (accessed on 20 March 2025)), through national funds (OE), within the scope of the Scientific Research and Technological Development Projects (IC&DT) program in all scientific domains (PTDC), through the Foundation for Science and Technology, I.P. (FCT, IP). The authors also acknowledge the partial financial support within the R&D Unit Project Scope: UIDB/04436/2020, UIDP/04436/2020 and LA/P/0029/2020. R.O.R. and P.S. thank FCT for their contract funding provided through 2020.03975.CEECIND ([[https://doi.org/10.54499/2020.03975.CEECIND/CP1600/CT0026](https://doi.org/10.54499/2020.03975.CEECIND/CP1600/CT0026)]([https://doi.org/10.54499/2020.03975.CEECIND/CP1600/CT0026](https://doi.org/10.54499/2020.03975.CEECIND/CP1600/CT0026)) (accessed on 20 March 2025)) and 2021.01086.CEECIND ([[https://doi.org/10.54499/2021.01086.CEECIND/CP1664/CT0008](https://doi.org/10.54499/2021.01086.CEECIND/CP1664/CT0008)]([https://doi.org/10.54499/2021.01086.CEECIND/CP1664/CT0008](https://doi.org/10.54499/2021.01086.CEECIND/CP1664/CT0008)) (accessed on 20 March 2025)), respectively. The original contributions presented in this study are included in the article/Supplementary Materials. Further inquiries can be directed to the corresponding author. The authors declare no conflicts of interest.