## 3 Materials and Methods
### Fractionation by Solid-Phase Extraction (SPE)  
The freeze-dried _D. vermicularis_ (FdDV) was extracted in methanol and dichloromethane (MeOH:DCM = 1:1, \(v/v\); 10 mL/g solvent-solid ratio) with 5 min of sonication (ultrasound-bath Elma, Elmasonic P 70 H, Singen, Germany; 37 kHz/50 W) three times. The gained extract (with 6.037 g/105.3 mg drug:extract ratio) was evaporated under a nitrogen flow (5.0, Messer, Croatia) and was then mixed with C18 powder (40-63 \(\upmu\)m, Macherey-Nagel Polygopper 60-50 C18, Fisher Scientific, Waltham, MA, USA). An SPE cartridge (C18 with particle size of 40 \(\upmu\)m, column capacity of 6 mL, and bed weight of 1 g, Agilent Bond Elut, Waldbronn, Germany) was conditioned with MeOH and ultrapure water and then covered with dry extract. To obtain the fractions F1 to F4, the sample was eluted by applying the solvents of decreasing polarity [10]: F1 (H\({}_{2}\)O), F2 (H\({}_{2}\)O:MeOH = 1:1, \(v/v\)), F3 (MeOH), and F4 (MeOH:DCM = 1:1, \(v/v\))). Less polar compounds were eluted in F3 and F4. The fractions were dried by SpeedVac (SPD1030, Thermo Scientific, Waltham, MA, USA) and stored at 4 \({}^{\circ}\)C in the dark before further analysis.  
Ultra-High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry (UHPLC-ESI-HRMS) of F3 and F4  
The UHPLC-ESI-HRMS analyses were performed using an UHPLC ExionLC AD system (AB Sciex, Concord, ON, Canada), which was equipped with the ExionLC Controller, ExionLC AD Pump, ExionLC AD Degasser, ExionLC solvent delivery system, ExionLC AD Autosampler, and ExionLC AD Column oven tandem quadrupole-time-of-flight (Q-TOF) mass spectrometer TripleTOF 6600+ (AB Sciex, Concord, ON, Canada) having a Duospray ion source. The chromatographic separations were achieved on the analytical column Acquity UPLC BEH Phenyl-Hexyl with dimensions of 2.1 mm \(\times\) 100 mm and a particle size of 1.7 \(\upmu\)m (Waters, Milford, MA, USA). The aqueous mobile phase (A) was water containing 0.1% formic acid and the organic mobile phase (B) was acetonitrile with 0.1% formic acid. The mobile phase flow rate was continuously 0.4 mL/min and the oven temperature was 30 \({}^{\circ}\)C. The elution started isocratically for 0.6 min with 2% of B and, later, the gradient program was applied: 0.6-18.5 min (B linear gradient to 100%), 18.5-25 min (100% B). An amount of 4 \(\upmu\)L of the sample was injected.  
Positive electrospray ionisation (ESI+) was set. Collision-induced dissociation (CID) in information-dependent acquisition (IDA) mode was applied recording the MS/MS mass spectra. The precursor ions only with the signal intensities above the 200 cps threshold were recorded with the maximum number of 15 precursor ions simultaneously subjected to CID. The parameters set in the ion source were as follows: source temperature of 300 \({}^{\circ}\)C, nebulising gas (gas 1, air) pressure of 40 psi, curtain gas (nitrogen) pressure of 30 psi, heater gas (gas 2, air) pressure of 15 psi, and ESI capillary voltage of 5.5 kV. Mass spectra were recorded in the m/z range of 100-1000 (MS) with the declustering potential of 80 V and accumulation time of 100 ms. MS/MS data were recorded in the m/z range of 20-1000, and the collision energy of the collision gas (nitrogen) was 40 eV with a spread of 20 eV. The mass scale calibrations (in the MS and MS/MS modes) were performed prior to each run in an automatic regime using an ESI Positive Calibration Solution 5600 (AB Sciex, Concord, ON, Canada).  
ACD/Spectrus Processor 2021.1.0. (ACD/Labs, Toronto, ON, Canada) was used for processing data. The accurate masses of the protonated molecules, their isotopic distributions, and the product ions m/z in MS/MS spectra were used for the compounds' elemental compositions determination. Detected components were then identified based on their mass spectra and the elemental compositions linked to search in the Chemical Entities of Biological Interest (ChEBI) database. The choice among the suggested hits was based on MS/MS data matching.