## 3 Results and Discussion
### Identification of Microorganisms in the Extraction Area and Juice Purification
\begin{table}
\begin{tabular}{c c c c c c c c c c} \hline \hline \multirow{2}{*}{**Periods**} & \multirow{2}{*}{**Process**} & \multirow{2}{*}{**Sampling Points**} & \multicolumn{4}{c}{**Reads**} & \multicolumn{4}{c}{**OTU**} \\ \cline{3-10}  & & & \multicolumn{2}{c}{**Rosin Acid**} & \multicolumn{2}{c}{**No Treatment**} & \multicolumn{2}{c}{**Rosin Acid**} & \multicolumn{2}{c}{**No Treatment**} \\ \cline{3-10}  & & & **Mean** & **SD** & **Mean** & **SD** & **Mean** & **SD** & **Mean** & **SD** \\ \hline \multirow{10}{*}{November} & \multirow{5}{*}{Extraction} & Fresh water & 12,228.00 & * & 15,911 & * & 4 & * & 9 & * \\  & & Press water & 15,090.50 & 10,115.16 & 3251.5 & 2305.88 & 16 & 1.41 & 11 & 1.41 \\  & & Juice of mid-tower 1 & 26,274.50 & 2267.69 & 19,095 & 24,744.49 & 13 & 0.00 & 3.5 & 2.12 \\  & & Juice of mid-tower 2 & 15,928.00 & 21,374.42 & 24,194.5 & 6767.72 & 12.5 & 0.71 & 2.5 & 0.71 \\  & & Tower juice 1 & 18,143.50 & 2551.95 & 12,786 & * & 13 & 1.41 & 12 & * \\  & & Tower juice 2 & 26,064.50 & 8954.09 & 12,136.5 & 692.26 & 19 & 1.41 & 9.5 & 4.95 \\  & & Cosette-juice mixture 1 & 15,843.00 & 3358.76 & 5182 & 6313.05 & 13.5 & 3.54 & 9 & 1.41 \\  & & Cosette-juice mixture 2 & 30,421.00 & 12,539.83 & 6596 & 927.24 & 32 & 12.73 & 10 & 9.90 \\  & & Raw juice & 3726.00 & 3445.02 & 10,562.5 & 8495.89 & 55 & 8.49 & 25 & 24.04 \\ \hline \multirow{10}{*}{December} & \multirow{5}{*}{Juice} & Hard juice & 178,524.00 & 28,550.14 & 17,3944 & 15,253.71 & 1 & 0.00 & 21 & 4.24 \\  & & Thin juice & 119,452.00 & 22,429.43 & 124,578 & 6853.28 & 1.5 & 0.71 & 1 & 0.00 \\  & & Eluat before UV & 18,015.00 & * & 15,325.5 & 4868.43 & 4 & * & 4 & 0.00 \\  & & Eluat after UV & 14,614.50 & 211.42 & 22,596.5 & 11,916.87 & 4 & 0.00 & 4 & 0.00 \\ \hline \multirow{10}{*}{November} & \multirow{5}{*}{Extraction} & Fresh water & 43,396.50 & 35,391.40 & 12,021.5 & 3995.86 & 3.5 & 0.71 & 4 & 0.00 \\  & & Press water & 9637.00 & 562.86 & 8569 & 1043.69 & 19.5 & 2.12 & 16.5 & 0.71 \\  & & Juice of mid-tower 1 & 26,288.50 & 3160.06 & 22,570 & 6057.08 & 14 & 5.66 & 7.5 & 4.95 \\  & & Juice of mid-tower 2 & 30,174.00 & * & 38,261.5 & 3270.37 & 13 & * & 2 & 0.00 \\  & & Tower juice 1 & 13,319.00 & 3798.58 & 11,126.5 & 1731.70 & 31.5 & 24.75 & 11 & 0.00 \\  & & Tower juice 2 & 15,014.50 & 7008.14 & 12,591 & 76.37 & 63.5 & 24.75 & 11 & 0.00 \\  & & Cosette-juice mixture 1 & 8181.00 & 10,504.78 & 11,426.5 & 1997.58 & 64.5 & 40.31 & 15.5 & 4.95 \\  & & Cosette-juice mixture 2 & 22,260.50 & 10,693.58 & 12,601 & 353.55 & 71 & 32.53 & 11 & 0.00 \\  & & Raw juice & 22,420.50 & 5838.58 & 7326.5 & 2069.70 & 119.5 & 4.95 & 70 & 12.73 \\ \hline \multirow{10}{*}{January} & \multirow{5}{*}{Extraction} & Hard juice & 143,327.00 & 10,534.48 & 123,280.5 & 84,686.64 & 18 & 14.14 & 19.5 & 4.95 \\  & & Thin juice & 145,599.00 & 2979.75 & 126,441 & 4167.69 & 1 & 0.00 & 1 & 0.00 \\  & & Eluat before UV & 70,983.50 & 86,489.77 & 208,905.5 & 100,409.87 & 6 & 2.83 & 8.5 & 2.12 \\  & & Eluat after UV & 10,739.00 & * & 42,854 & 28,629.34 & 6 & * & 7 & 2.83 \\ \hline \multirow{10}{*}{January} & \multirow{5}{*}{Extraction} & Fresh water & 10,228.00 & * & 43,980 & 34,129.22 & 4 & * & 21 & 24.04 \\  & & Press water & 11,162.50 & 3650.79 & 9279.5 & 1177.33 & 18.5 & 2.12 & 14 & 0.00 \\  & & Juice of mid-tower 1 & 18,860.50 & 10,550.74 & 36,115.5 & 1593.11 & 11.5 & 3.54 & 12.5 & 3.54 \\ \cline{1-1}  & & Juice of mid-tower 2 & 1852.50 & 1697.76 & 32,030 & 4236.98 & 29.5 & 6.36 & 9 & 4.24 \\ \cline{1-1}  & & Tower juice 1 & 15,531.50 & 4796.31 & 26,656 & 11,800.00 & 88 & 1.41 & 13 & 2.83 \\ \cline{1-1}  & & Tower juice 2 & 20,080.00 & 12,682.67 & 17,257 & 415.78 & 116.5 & 4.95 & 14 & 1.41 \\ \cline{1-1}  & & Cosette-juice mixture 1 & 25,381.50 & 6385.88 & 14,483 & 2616.30 & 99 & 7.07 & 17 & 2.83 \\ \cline{1-1}  & & Cosette-juice mixture 2 & 19,220.50 & 12,271.84 & 28,385 & 11,631.91 & 114 & 4.24 & 18.5 & 2.12 \\ \cline{1-1}  & & Raw juice & 79,043.50 & 47,853.45 & 24,697 & 7937.98 & 94.5 & 17.68 & 98 & 16.97 \\ \cline{1-1} \cline{1-1  
\begin{table}
\begin{tabular}{c c c c c c c c c c c c} \hline
**Genus** & **Family** & **Order** & **Class** & **Phylum** & **Gram** & **Gram** & **Grown Temperature** & **Relation to** & **Spore** & **Sample Point** & **Literature** \\ \hline _Tambrechallins_ & _Alicyclobellaceae_ & _Reallalles_ & _Reall_ & _Firmatics_ & positive & 5-37 °C & aerobic & + & 2 & [33] \\ _Reallalles_ & _Reallalles_ & _Reallall_ & _Firmatics_ & positive & 35-50 °C & aerobic & + & 2 & [34] \\ _Reallalles_ & _Reallalles_ & _Reallall_ & _Firmatics_ & positive & 25-65 °C & facultvely & + & 2 & [35] \\ _Reallalles_ & _Reallalles_ & _Reallall_ & _Firmatics_ & positive & 42-70 °C & anaerobic & + & 5.1; 5; 5; 42; 4; 42 & [34, 36] \\ _Reallalles_ & _Reallallence_ & _Reallalles_ & _Reallall_ & _Firmatics_ & positive & 45-70 °C & aerobic & + & 5.1; 5; 42; 41; 42 & [34, 37, 38] \\ _Reallalles_ & _Reallalles_ & _Reallallall_ & _Firmatics_ & positive & 55-65°C & aerobic & + & 5.1; 5; 5; 42; 41; 42 & [34, 37, 38] \\ _Reallalles_ & _Reallallalles_ & _Reallallall_ & _Firmatics_ & positive & 40-65°C & aerobic & + & 5.1; 5; 5; 42; 41; 42 & [39] \\ _Reallallalles_ & _Reallallalles_ & _Reallall_ & _Firmatics_ & positive & 40-65°C & aerobic & + & 31; 32; 41; 42 & [40] \\ _Reallalles_ & _Leacontee_ & _Leacontee_ & _Leuchicalles_ & _Reallall_ & _Firmatics_ & positive & 20-30 °C & anaerobic & \(-\) & 6 & [41] \\ _Leacontee_ & _Leacontee_ & _Leacontee_ & _Leuchicalles_ & _Reallall_ & _Firmatics_ & positive & 20-30 °C & anaerobic & \(-\) & 6 & [41] \\ _Leacontee_ & _Leacontee_ & _Leacontee_ & _Leuchicalles_ & _Reallall_ & _Firmatics_ & positive & 20-30 °C & anaerobic & \(-\) & 6 & [41] \\ _Leacontee_ & _Leacontee_ & _Leacontee_ & _Leuchicalles_ & _Reallall_ & _Firmatics_ & positive & 20-30 °C & anaerobic & \(-\) & 6 & [41] \\ _Leacontee_ & _Leacontee_ & _Leacontee_ & _Leuchicalles_ & _Reallall_ & _Firmatics_ & positive & 20-30 °C & anaerobic & \(-\) & 6 & [41] \\ _Reallalles_ & _Leacontee_ & _Leacontee_ & _Leuchicalles_ & _Reallall_ & _Firmatics_ & positive & 20-30 °C & aerobic & \(-\) & 6 & [42] \\ _Reallalles_ & _Leuchicalles_ & _Leuchicalles_ & _Reallall_ & _Firmatics_ & positive & 45°C, not at 45 °C & anaerobic & \(-\) & 3.1 & [43] \\ _Reallalles_ & _Leuchicalles_ & _Leuchicalles_ & _Reallall_ & _Firmatics_ & positive & at 45 °C, not at 15 °C & micro & \(-\) & 2, 3.1 & [43] \\ _Reallallalles_ & _Leuchicalles_ & _Leuchicalles_ & _Reallall_ & _Firmatics_ & positive & at 45 °C, not at 15 °C & \(\) & & & & & & \\ _Reallallalles_ & _Leuchicalles_ & _Leuchicalles_ & _Reallall_ & _Firmatics_ & positive & at 45 °C, not at 15 °C & anaerobic & \(-\) & 2 ; 6 & [43] \\ _Reallallalles_ & _Leuchicalles  
Raw juice represents the sample with rather low temperature in the extraction area owing to the countercurrent processing principle resulting in highest alpha diversity (Table 3, Figure S1 and Table S1).