## 4 Conclusions  
Capturing the dynamics of cells and their nuclei is critical to understanding normal cell function and fundamental cell biology, and to ultimately predict the response of living organisms to stimuli. Microgravity and hypergravity are two mechanical stimuli that humans and plants are exposed to on space missions. Several studies have been performed using live-cell imaging capability on the ISS and sounding rocket true microgravity platforms to understand the response of human cells to microgravity [30; 31]. In order to provide a more cost-effective and easily accessible platform to researchers, we developed a live-imaging module to capture the dynamics of cells in SMG. A rotating machine provides a challenging environment for any optical-mechanical setup because loading levels continuously change. Accelerations and rotation direction changes dramatize the condition by introducing mechanical shocks. The module described herein successfully addresses these challenges and permits the live imaging of cells over prolonged intervals.  
The following are available online at [[https://www.mdpi.com/article/10.3390/app11073122/s1](https://www.mdpi.com/article/10.3390/app11073122/s1)]([https://www.mdpi.com/article/10.3390/app11073122/s1](https://www.mdpi.com/article/10.3390/app11073122/s1)), Video S1.  
Conceptualization, S.N. and Y.Z.; methodology, S.N., A.L., C.U.; software, A.L., C.U. and M.A.L.; validation, S.N., A.L. and M.A.L.; formal analysis, S.N. and A.L.; investigation, S.N., A.L. and C.U.; resources, Y.Z. and H.G.L.; data curation, Y.Z. and H.G.L.; writing--original draft preparation, S.N. and A.L.; writing--review and editing, Y.Z., H.G.L. and S.N.; visualization, Y.Z., S.N. and A.L.; supervision, S.N. and Y.Z.; project administration, S.N. and Y.Z.; funding acquisition Y.Z. and H.G.L. All authors have read and agreed to the published version of the manuscript.  
The MSSF and this development were funded by the NASA Space Biology Program. [PERSON] was supported by the NASA Post-doctoral (NPP) program (NNH15C048B) administered by Universities Space Research Association (USRA) for the whole development and manuscript writing, and her participation in editing and revision was supported by the NASA Space Biology program, Grant #80 NSSC20K0423. [PERSON] and [PERSON] were supported by the NASA Space  
Figure 4: Fluorescently labelled nuclei of AG01522 cells in SMG. The image was captured using the live-imaging module with a AM7515 MT8A-GFBW fluorescence 5 MP Dino-Lite Edge Series microscope mounted on an RPM. The live cells pre-labeled with SYTO 9 Nucleic Acid dye were excited with 488 nm LEDs to acquire the green fluorescent image.  
Biology Program through the NASA internship program. This development was submitted in the NASA New Technology Reporting System as KSC-14221 on 28 September 2018, entitled \"Modules to Support Live Microscopic Imaging and Samples on Ground-Based Microgravity Simulator Devices\".  
Not applicable.  
We extend our sincere appreciation to [PERSON], University of Wisconsin-Madison, for his administrative support. We would like to thank [PERSON], [PERSON], [PERSON], [PERSON], and [PERSON] for providing logistics and MSSF laboratory support.  
There are no conflict to declare.