## 3 Results and Discussion
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Treatment Groups** & **n** & **Minimum** & **Maximum** & **Mean** & **Std.** & **_p_ \\ \hline Soil 30\% + Chlorine 0.75 & 10 & 41 & 56 & 49.4 & 5.80 & 0.147 \\ \hline Soil 30\% + Chlorine 0.375 & 10 & 51 & 71 & 61.7 & 6.48 & 0.929 \\ \hline Chlorine 0.75 + Soil 30\% & 10 & 53 & 124 & 86.7 & 22.06 & 0.227 \\ \hline Soil 30\% + Chlorine 0.1875 & 10 & 78 & 103 & 93.3 & 8.62 & 0.495 \\ \hline Chlorine 0.375 + Soil 30\% & 10 & 36 & 300 & 126.8 & 67.46 & 0.660 \\ \hline Chlorine 0.1875 + Soil 30\% & 10 & 104 & 208 & 153.6 & 37.38 & 0.125 \\ \hline Soil 30\% + Chlorine 0.09375 & 10 & 188 & 298 & 245.5 & 37.40 & 0.965 \\ \hline Chlorine 0.09375 + Soil 30\% & 10 & 170 & 348 & 271.2 & 60.00 & 0.003 \\ \hline Soil 30\% + Chlorine 0.046 & 10 & 276 & 350 & 307.4 & 23.95 & 0.318 \\ \hline Chlorine 0.046 + Soil 30\% & 10 & 280 & 420 & 348.5 & 54.16 & 0.370 \\ \hline Soil 30\% + Water & 10 & 300 & 500 & 419.0 & 78.80 & 0.092 \\ \hline Water + Soil 30\% & 10 & 300 & 500 & 419.0 & 78.80 & 0.125 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Distribution of the decrease in the number of bacteria by treatment group. Figure 1: Bacterial morphology of _Bacillus cereus_, assessed with fluorescence and Gram staining. (**a,c,e,g,i**) fluorescent staining of the bacterial morphology at 0.75, 0.375, 0.188, 0.094, and 0.047 ppm NaOCL concentrations, respectively. (**b,d,f,h,j**) Gram staining of the bacterial morphology at 0.75, 0.375, 0.188, 0.094, and 0.047 ppm NaOCL concentrations, respectively. (**k**) Fluorescent staining of the bacterial morphology using water. (**l**) Gram staining of the bacterial morphology using water. Figure 2 demonstrates that the number of colony-forming units (CFUs) of bacteria was 1450 before receiving treatment. Following the administration of therapy, each combination could produce a reduction in the number of bacteria, the extent of which ranged from 49 CFUs to 419 CFUs. The number of CFUs of bacteria only dropped to 1210 in the group that served as the control. The combination of these treatments achieved a sizeable drop in the bacterial population. Table 3 demonstrates how effectively the treatment reduced the total number of bacteria in the environment. The data presented in the table demonstrate that there was a reduction in the number of bacteria that were able to survive as a result of the interaction between the soil concentration and the NaOCl dose, as well as the sequential application of these two variables. The treatment that consisted of a mixture of 30% dirt and 0.75 ppm chlorine was the one that was able to lower the number of bacteria by the greatest amount out among all the available options. When syringe waste from hospitals was treated with soil containing 30% chlorine and 0.75 ppm chlorine, the amount of bacteria that was removed was 1161 CFU lower than that of the control group. The use of the combined treatment caused a 96% reduction in the bacterial count. The ability of the combination of the soil concentration and NaOCl dose to clean bacteria was ordered from large to small, as shown in the table, and the combination treatment consisting of water and soil with a concentration of 30% was the treatment that exhibited the lowest variety in terms of cleaning bacteria. This treatment was able to reduce the number of bacteria by 791 CFU. The combined treatment had the potential to kill 93% of the germs. According to the findings of [32], reducing the concentration of NaOCl to 0.2 mg-Cl\({}_{2}\)/L and using a contact period of 30 min enabled the researchers to cut the number of bacteria by 90 per cent. Another research group [19] found that the suggested level of NaOCl, which was 0.5 mg/L, was less effective in reducing the number of _Escherichia coli_ isolated from two wastewater treatment plants; they were still present in the water. A treatment  
Figure 2: Bacteria reduction before and after treatment. in which the concentration of NaOCl was 1.5 mg/L was found to be more successful in eliminating _Escherichia coli_ bacteria.