## 1 Introduction  
Yeast is a very important eukaryotic microorganism in the food industry because of its excellent ability to ferment carbohydrates [1]. Among them, the budding yeast _Saccharomyces cerevisiae_ is highly safe as a microorganism that has been used in fermentation production of bread, sake, and beer since ancient times [1]. Therefore, _S. cerevisiae_ is a familiar strain to industry, so that many studies have been enthusiastically conducted on its life cycles [2, 3], genetic engineering [4], the controlling ploidy such as haploid and diploid [5], and improving the activities of its growth and fermentation [6]. In particular, BY4741 and BY4742, prepared as experimental strains of _S. cerevisiae_, are haploid, so the effects of the introduced genes are readily expressed in the phenotype [7]; those strains have _his3_, _lu2_, _met15_, and _una3_ deleted as the essential genes for life maintenance [8], so that those are used in genetic engineering as the nutritional requirement markers, with the result that there are many reports of the creation of mutants by using the plasmid vector system and genome integration system and of the evaluation of the activities of those strains [9]. Although many reports regarding those experimental strains do indeed exist, traditional selective media are still mostly used [10]. A commonly used selection medium for _S. cerevisiae_ strains like BY4741 is synthetic dextrose medium (SD medium with essential nutrients (YG-group medium, as it is termed in this study)), which contains glucose as a carbon source, yeast nitrogen base without amino acid (YNB) as a nutrient source, and other elements to meet the nutritional requirements of each _S. cerevisiae_ genotype [10]. However, this synthetic medium is expensive, and YNB, which is a constituent nutrient, is extremely expensive. As of August 2023, the YNBs were 14,096 JPY per 100 g by Funakoshi Co., Ltd. (Tokyo, Japan), 16,500 JPY by Merck KGaA (Darmstadt, Germany), and 15,600 JPY by Nacalai Tesque Inc. (Kyoto, Japan). YNB popularly contains salts (KH\({}_{2}\)PO\({}_{4}\) 1000 mg\(\cdot\)L\({}^{-1}\); MgSO\({}_{4}\) 500 mg\(\cdot\)L\({}^{-1}\); NaCl 100 mg\(\cdot\)L\({}^{-1}\); CaCl\({}_{2}\) 100 mg\(\cdot\)L\({}^{-1}\)), vitamins (biotin 0.002 mg\(\cdot\)L\({}^{-1}\); Ca pantotheane 0.4 mg\(\cdot\)L\({}^{-1}\); folic acid 0.002 mg\(\cdot\)L\({}^{-1}\); inositol 2.0 mg\(\cdot\)L\({}^{-1}\); niacin 0.4 mg\(\cdot\)L\({}^{-1}\); PABA 0.2 mg\(\cdot\)L\({}^{-1}\); pyridoxine-HCl 0.4 mg\(\cdot\)L\({}^{-1}\); riboflavin 0.2 mg\(\cdot\)L\({}^{-1}\); thiamine-HCl 0.4 mg\(\cdot\)L\({}^{-1}\)), and trace elements (boric acid 0.5 mg\(\cdot\)L\({}^{-1}\); CuSO\({}_{4}\) 0.04 mg\(\cdot\)L\({}^{-1}\); KI 0.1 mg\(\cdot\)L\({}^{-1}\); FeCl\({}_{3}\) 0.2 mg\(\cdot\)L\({}^{-1}\); MnSO\({}_{4}\) 0.4 mg\(\cdot\)L\({}^{-1}\); Na\({}_{2}\)MoO\({}_{4}\) 0.2 mg\(\cdot\)L\({}^{-1}\); ZnSO\({}_{4}\) 0.4 mg\(\cdot\)L\({}^{-1}\)) [11], and replacing YNB with cheaper nutrient sources could greatly reduce the economic cost of experimentation with _S. cerevisiae_ strains like BY4741. Food residues containing various elements are powerful nutrient sources for cultivating _S. cerevisiae_, and wheat bran, which is rich in nutrient sources such as vitamins and minerals, was focused on in this study. In addition, wheat bran has an advantage in price [12; 13]. Moreover, if wheat bran could be used as a nutrient source in a restricted medium instead of YNB, the cost of culturing experimental _S. cerevisiae_ strains such as BY4741 in the restricted medium could be greatly reduced; however, there have been few reports regarding the use of wheat bran as a nutrient, meaning that the cell responses of _S. cerevisiae_ in medium containing wheat bran have not been clear. Therefore, the investigation of the effects on _S. cerevisiae_ of the use of wheat bran as a nutrient source has significance for the field in microorganisms. In the use of wheat bran, the evaluation of the effects on fermentation by _S. cerevisiae_ especially should be deeply analyzed because _S.