# Effect of Ultrasound on Fermentation of Thick Molasses Worts by Distiller's Yeast
## 3 Results and Discussion
### Wort Analysis
#### 3.2.3 Influence of Ultrasound Treatment on Fermentation Dynamics  
One of the main objectives of this research was to check whether the planned microwave treatment of the fermented word would affect the dynamics of fermentation. The following graphs (Figures 1-3) show the percentage weight loss of the fermented samples. One hundred percent is the assumed mass of CO\({}_{2}\) that would be produced according to the stoichiometry of the fermentation reaction of the sugars present in the medium before fermentation. When discussing the results of mass losses, we assumed that due to the fact that the fermentation flasks were secured with stoppers equipped with glycerine-filled \begin{table}
\begin{tabular}{c c c c c} \hline \hline
**g/L** & **SucA** & **LacA** & **AceA** & **GlycOH** \\ \hline \multicolumn{5}{c}{18 \({}^{\circ}\)Blg} \\ \hline ER contr & 1.56 \(\pm\) 0.08 & 2.03 \(\pm\) 0.08 & 1.87 \(\pm\) 0.04 & 7.92 \(\pm\) 0.21 \\ \hline ER u50 & 1.76 \(\pm\) 0.04 & 2.12 \(\pm\) 0.06 & 2.10 \(\pm\) 0.08 & 8.74 \(\pm\) 0.26 \\ \hline ER u75 & 1.59 \(\pm\) 0.05 & 2.25 \(\pm\) 0.04 & 1.98 \(\pm\) 0.11 & 8.51 \(\pm\) 0.21 \\ \hline TD contr & 1.60 \(\pm\) 0.03 & 2.10 \(\pm\) 0.06 & 1.96 \(\pm\) 0.06 & 7.83 \(\pm\) 0.16 \\ \hline TD u50 & 1.62 \(\pm\) 0.06 & 2.00 \(\pm\) 0.06 & 1.92 \(\pm\) 0.07 & 8.17 \(\pm\) 0.08 \\ \hline TD u75 & 1.56 \(\pm\) 0.05 & 1.86 \(\pm\) 0.06 & 1.59 \(\pm\) 0.04 & 6.96 \(\pm\) 0.09 \\ \hline \multicolumn{5}{c}{22 \({}^{\circ}\)Blg} \\ \hline ER contr & 1.92 \(\pm\) 0.04 & 3.03 \(\pm\) 0.03 & 2.51 \(\pm\) 0.08 & 9.80 \(\pm\) 0.46 \\ \hline ER u50 & 1.94 \(\pm\) 0.05 & 2.75 \(\pm\) 0.10 & 2.52 \(\pm\) 0.05 & 10.29 \(\pm\) 0.29 \\ \hline ER u75 & 2.05 \(\pm\) 0.07 & 3.01 \(\pm\) 0.06 & 2.54 \(\pm\) 0.09 & 10.17 \(\pm\) 0.29 \\ \hline TD contr & 1.67 \(\pm\) 0.08 & 2.89 \(\pm\) 0.08 & 2.33 \(\pm\) 0.08 & 9.27 \(\pm\) 0.35 \\ \hline TD u50 & 1.61 \(\pm\) 0.06 & 2.71 \(\pm\) 0.11 & 2.47 \(\pm\) 0.04 & 9.76 \(\pm\) 0.20 \\ \hline TD u75 & 1.52 \(\pm\) 0.04 & 2.71 \(\pm\) 0.07 & 2.16 \(\pm\) 0.07 & 8.52 \(\pm\) 0.14 \\ \hline \multicolumn{5}{c}{24 \({}^{\circ}\)Blg} \\ \hline ER contr & 1.46 \(\pm\) 0.05 & 3.56 \(\pm\) 0.13 & 2.62 \(\pm\) 0.09 & 8.36 \(\pm\) 0.21 \\ \hline ER u50 & 1.59 \(\pm\) 0.05 & 3.46 \(\pm\) 0.12 & 2.56 \(\pm\) 0.06 & 8.44 \(\pm\) 0.20 \\ \hline ER u75 & 1.52 \(\pm\) 0.07 & 3.19 \(\pm\) 0.15 & 2.31 \(\pm\) 0.05 & 8.19 \(\pm\) 0.23 \\ \hline TD contr & 1.26 \(\pm\) 0.06 & 3.30 \(\pm\) 0.12 & 2.04 \(\pm\) 0.07 & 5.06 \(\pm\) 0.10 \\ \hline TD u50 & 1.35 \(\pm\) 0.05 & 3.60 \(\pm\) 0.06 & 2.25 \(\pm\) 0.08 & 5.74 \(\pm\) 0.19 \\ \hline TD u75 & 1.21 \(\pm\) 0.04 & 3.53 \(\pm\) 0.10 & 2.02 \(\pm\) 0.09 & 5.37 \(\pm\) 0.14 \\ \hline \end{tabular}
\end{table} 
Table 6: Selected acids and glycerol after 72 h of fermentation of 18, 22, and 24 \({}^{\circ}\)Blg words. tubes, the loss of mass associated with the evaporation of water from the words could be disregarded. The results of the fermentation dynamics of word using two tested strains and different levels of ultrasonic treatment during fermentation provided extremely interesting material for observation and discussion. The above diagrams (Figures 1-3) summarise the fermentation dynamics of molasses word with an initial density of 18, 22, and 24 \({}^{\circ}\)Blg fermented by Ethanol Red or Thermosac Dry yeast. During fermentation, the samples were subjected to 24 kHz ultrasound or not. The results show the percentage of weight loss in the samples. The mass value of CO\({}_{2}\) that would be released according to the fermentation equation with complete fermentation of the sugars available in the word of a given density was taken as 100% loss. The graph for the 18 \({}^{\circ}\)Blg (Figure 1) wor  
Figure 1: Percentage release of carbon dioxide during fermentation of 18 \({}^{\circ}\)Blg word depending on ultrasound treatment and yeast strain. Figure 2: Percentage release of carbon dioxide during fermentation of 22 \({}^{\circ}\)Blg word depending on ultrasound treatment and yeast strain.