## 3 Results and Discussion
### Analysis of Plasmid Presence and Antibiotic Resistance Genes
#### 3.6.4 Indole-Related Gene Clusters
\begin{table}
\begin{tabular}{c c c c} \hline
**GenBank** & **Putative Functions** & **Proteins (Amino Acids)** &
\begin{tabular}{c} **Identity/** \\ **Similarity** \\ \end{tabular} \\ \hline XJJ15611 & LuxR-like transcription regulator & LjeR (917 aa)/AbeR (932 aa) & 79.06\%/86.18\% \\ XJJ15612 & RebP-like cytochrome P450 & LjeP (400 aa)/AbeP (392 aa) & 86.60\%/92.06\% \\ XJJ15613 & RebC-like FAD-monooxygenase & LjeC (525 aa)/AbeC (533 aa) & 89.87\%/94.56\% \\ XJJ15614 & RebO-like tryptophan oxidase & LjeC (501 aa)/AbeO (513 aa) & 90.27\%/94.36\% \\ XJJ15615 & Alpha/beta hydrolase & LjeY (262 aa)/AbeY (264 aa) & 86.74\%/91.67\% \\ XJJ15616 & Flavin reductase family protein & LjeF (160 aa)/AbeF (160 aa) & 89.38\%/92.50\% \\ XJJ15617 & Cation/proton antiporter & LjeT (411 aa)/AbeT (412 aa) & 84.93\%/90.19\% \\ XJJ15618 & Tryptophan halogenase & LjeH (515 aa)/AbeH (515 aa) & 95.15\%/97.09\% \\ XJJ15619 & O-methyltransferasease & LjeM3 (336 aa)/AbeM3 (336 aa) & 89.32\%/93.77\% \\ XJJ15620 & FAD-dependent oxidoreductase & LjeX2 (413 aa)/AbeX2 (407 aa) & 92.03\%/96.14\% \\ XJJ15621 & Chromopyrrolic acid synthase & LjeD (1012 aa)/AbeD (1015 aa) & 93.10\%/96.45\% \\ XJJ15622 & N-methyltransferase transferase & LjeM1 (231 aa)/AbeM1 (231 aa) & 90.91\%/95.67\% \\ XJJ15623 & FAD-dependent monooxygenase & LjeX1 (532 aa)/AbeX1 (533 aa) & 93.43\%/96.81\% \\ XJJ15624 & N-methyltransferasease & LjeM2 (230 aa)/AbeM2 (230 aa) & 91.30\%/93.91\% \\ \hline \end{tabular}
\end{table} 
Table 2: BE-54017 BGC of _Lentzea_ sp. JNUC C0626. Figure 6: Genetic organization of the BE-54017 gene cluster within BGC region 21 of the _Lentzea_ sp. JNUCC 0626 genome. In the antiSMASH analysis, genes XJJ15611 and XJ15612, initially omitted, are now annotated as encoding a LuxR-like transcriptional regulator and a RebP-like cytochrome P450, respectively. In the upstream region, four genes (XJJ15625 to XJ15628) show homology to a caspase family protein, a hypothetical protein, a Toll/interleukin-1 receptor domain-containing protein, and an adhesin. mechanisms. These transposases can replicate through a \"copy-remove-insert\" mechanism, allowing copies to integrate into new positions within the genome, which facilitates the dissemination of the same IS element through replication and homologous recombination, potentially resulting in the formation of minicycles or dimers [55]. Such repetitive sequences may be inherited as part of the genomic architecture and can assist in promoting genomic rearrangements or horizontal gene transfer. Additionally, the existence of multiple identical copies may be explained by selective pressure, where specific transposases contribute to adaptive functions such as antibiotic resistance or stress responses, which can lead to the retention of identical copies in the genome over time. This phenomenon is commonly observed in bacterial populations facing rapid environmental changes or selective pressures [56; 57; 58]. These findings underscore the adaptability of IS elements such as IS30, suggesting that they not only aid bacteria in responding to environmental challenges, but also contribute to genomic diversity.