## 3 Results
### Soil Enzymes Response to DO and G  
One of the main research objectives of the experiment was to determine the effects of DO and G on soil enzyme activity (Tables 3 and S1). The response of individual enzymes was related to the type of oil product pressure. Although both products, DO and G, destabilized the enzymatic properties of the soil, their direction of influence was opposite.  
Diesel oil acted as a stimulator of dehydrogenases, catalase, alkaline phosphatase, \(\beta\)-glucosidase, and arylsulfatase, while inhibiting acid phosphatase. Conversely, gasoline served as an inhibitor for all enzymes. Therefore, the influence factors of DO (IF\({}_{\text{DO}}\)) on the activity of individual enzymes, except for acid phosphatase, were positive, whereas those of G (IF\({}_{\text{G}}\)) were negative (Figure 4). Generally, higher positive or negative values of these indices were induced by the implementation of both tested products at a dose of 16 cm\({}^{3}\) compared to 8 cm\({}^{3}\) kg\  
The implementation of dolomite to the control soil (C) increased the activity of all enzymes except AcP and Glu (Figure 5a). It most significantly stimulated Ure (IF 2.780), AIP (IF 1.222), and Aryl (IF 1.023). It did not only affect the activity of AcP and Glu. It also increased the activity of all enzymes, except AcP and Glu, in soil contaminated with diesel oil and gasoline. A particularly high intensification of activity was observed in the G_16 soil, where the IF\({}_{\rm D}\) index ranged from 0.467 (Cat) to 7.69 (Deh) and 4.80 (Ure).  
Figure 4: Influence indices of diesel oil (IF\({}_{\rm D}\)) and gasoline (IF\({}_{\rm G}\)) on soil enzyme activity. Explanations of abbreviations are provided in Figure 1 and Table 2.  
Perlite exerted a significantly reduced effect on soil enzyme activity compared to dolomite (Figure 5b). In the control treatment (C), it stimulated the activity of AIP (IF 0.625), Cat (IF 0.289), Deh (IF 0.180), and Aryl (IF 0.114), whereas it inhibited the activity of AcP (IF \(-\)0.216). This sorbent had a marginal effect on enzyme activity in soil contaminated with gasoline at a dose of 8 cm\({}^{3}\) kg\({}^{-1}\) of soil. The highest IF value for this substance was observed for AIP (0.246) and Cat (0.158), while the lowest was observed for AcP (\(-\)0.170). Increased efficacy of perlite was observed in soil exposed to gasoline applied at a dose of 16 cm\({}^{3}\) kg\({}^{-1}\) of soil (G 16). Perlite stimulated the activity of all enzymes except for Ure and Glu. It stimulated the activity of Deh, Aryl, and AIP the most, with IF values for these enzymes of 3.667, 0.680, and 0.461, respectively. The sorbent tested was less effective in influencing the enzymes in soil contaminated with diesel oil. In the most heavily contaminated soil (DO_16), it led to a notable increase in the IF value for Deh (0.572) and a decrease for Ure (\(-\)0.323). A similar trend was also observed in the soil of treatment DO_8, although with an additional stimulation of Cat (IF 0.347) in this soil.  
Figure 5: Influence index: (**a**) dolomite (IF\({}_{\text{D}}\)) and (**b**) perlite (IF\({}_{\text{P}}\)) on soil enzyme activity. Explanations of abbreviations are provided in Figure 1 and Table 2.