## 4 Discussions  
The management of the complicated disruptive wound healing processes is a real challenge that is confronted with limitations of the health systems. Thus, effort is made to identify low costs alternatives, especially of natural origin, efficient activators of the complex process of wound healing. Medicinal plants, with high content of polyphenolic compounds, are an important approach to reduce the high levels of oxidative stress that, in most cases, affects the healing process. Hence, in our study, we focused on the phenolic profile of our extracts, in correlation with their potential antioxidant activities. Likewise, the DPPH radical scavenging activity assay was performed in order to evaluate the antioxidant capacity of the three _Artemisia_ extracts. A great number of natural compounds are also essential as antimicrobial agents, next to stimulating skin cell proliferation, migration or modelling the disruptive vascular process, thus, acting as multiple targeting active agents in the wound microenvironment [50; 51; 52]. In the current study, we selected three _Artemisia_ species (_A. annua_, _A dracunculus_ and _A. absinthium_) with a background in traditional skin ailment therapy and available on the herbal market of our country [53]. Confirmatory studies of the potential benefits in wound  
Figure 6: Stereomicroscopic images showing the effects induced by the _Artemisia_ extracts on the CAM using the anti-irritative HET-CAM assay; images represent the initial moment, before application of the irritant SDS 0.5% (\(t_{0}\)), and 5 min after application of the irritant (\(t_{5}\)); DMSO represents the solvent control and indomethacin, the anti-inflammatory control; scale bars represent 500 \(\upmu\)m. \begin{table}
\begin{tabular}{c c} \hline \hline
**Samples** & **Irritative Score** \\ \hline AAn & 18.38 \\ ADr & 19.01 \\ AAb & 19.69 \\ DMSO & 20.13 \\ SDS & 20.36 \\ Indometacin & 16.74 \\ \hline \hline \end{tabular}
\end{table} 
Table 3: The irritative scores of the analyzed samples in the anti-irritative HET-CAM assay. healing and regenerative medicine are still lacking; therefore, we intended to contribute with data concerning the phytochemical and bioactive potential of ethanolic extracts of the three _Artemisia_ species. Based on previous studies [54; 55; 56] that showed important content in polyphenolic compounds and lacking potential topical toxicity due to residual solvent remanence after drying, we selected to explore here the content in phenolic compounds of the 80% ethanolic extracts. The influence of the solvent on the antioxidant potential and chemical composition of _Artemisia annuu_ leaves extract was investigated by [PERSON] et al. [54]. In terms of TPC, they concluded that the most efficient solvent for the extraction of phenolics from the leaves of AAn are methanol and water, and the less efficient were chloroform and hexane. In the case of the methanolic extract, the TPC was of 134.50 \(\pm\) 4.37 mg/g. The extract of _Artemisia annuae herba_ tested in our study contains 129.28 \(\pm\) 2.09 mg GAE/g dry extract, using an ethanolic extract and the whole aerial part of the plant. [PERSON] et al. [55] evaluated the antioxidant potential and the phenolics found in various dried and fresh plants, tarragon (_Artemisia dracunculus_ L.) being one of them. For the extracts preparation the aerial part of the plant and 70% (\(\
u\)/\(\
u\)) ethanol were used and the TPC was 32.91 \(\pm\) 0.68 g GAE/kg extract in case of fresh plant material, while, in the case of dried tarragon, 42.53 \(\pm\) 0.93 g GAE/kg extract was obtained. Others obtained TPC values for methanolic tarragon extract in the range 97.2-253.5 mg GA/g, with a mean value of 151.6 mg GA/g, while for the water extract the range was 59.5-198.3 mg GA/g, and the mean value 102.8 mg GA/g [56]. Therefore, it can be said that the methanolic extract has the closest value to the one obtained by us for the _A. dracunculus_ total phenolic content. The regional area of _A.