## 2 Materials and Methods
### Clinical Protocol  
The study consisted of 5 visits divided into 3 phases: selection and pre-fitting, CL testing and post-fitting follow-up. The tests carried out in each of the study visits are shown in Table 1. The visual evaluation included measurement of monocular and binocular corrected visual acuity (VA) under mesopic conditions at a distance (6 m, optotype projector) and near distances (40 cm, Early Treatment Diabetic Retinopathy Study (ETDRS) test). The subjective refractive examination was based on the objective refraction obtained by means of static retinoscopy and subsequently performing the subjective examination in the trial frame following the standard procedure until reaching the maximum positive value providing the maximum visual acuity. The accommodative system was assessed by analyzing the accommodative response through dynamic retinoscopy using two different methodologies, the monocular estimation method (MEM) and the Nott method [26]. Three consecutive measures were obtained and the mean value reported. The evaluation of the binocular system was performed by analyzing three consecutive times the magnitude of the phoria at a distance and near with the cover test (CT), as well as by measuring the horizontal fusional versgences (FVs) using the prism bar, reporting the points of blurring, fusional break and fusional recovery [27]. Both VL and VC were evaluated by performing three measurements in each eye for each VF. Both the base-in (divergence) and base-out (convergence) fusional versgences at a distance and near were measured three times, and the mean value was reported, as recommended [27]. The stereoscopic vision was evaluated by means of the Random Dot 1S Stereo Acuity Test (Vision Assessment Corporation, Elk Grove Village, IL, USA).  
The slit lamp biomicroscopic examination was used in the first visit to examine the anterior segment and rule out any ocular pathology. In the CL testing, this examination allowed the clinician to assess the centration and movement of the CL to optimize its fitting. In addition, in the post-fitting follow-up, the slit lamp examination was needed to check the anterior segment health and to detect any potential complication that could be related to the CL wear, such as the presence of limbal vascularization, punctata keratitis of the formation of deposits over the CL surface.  
Subjective vision quality was assessed with a self-developed questionnaire asking about the perception of three photic phenomena, halos, glare and starbursts. Specifically, subjects were asked about the frequency (never, occasionally, frequently, often) and level of bothersomeness (none, mild, moderate, severe) of these three photic phenomena. The Visionix VX120 + multi-diagnostic platform (Luneau Technology, Chartres, France) was used to obtain keratometric data, corneal diameter, corneal pachymetry, anterior chamber depth (ACD), puppillometry and total ocular aberrations for 2 pupil apertures (3 and 5 mm). The IOL Master 500 (Carl Zeiss Meditec, Jena, Germany) was used to obtain the measurement of AXL, performing at least 6 consecutive measurements per eye and ensuring that all of them have an associated value of SNR (signal-to-noise ratio) of more than 3 units.  
All visits and measurements were performed during the same period of the day, between 17:30 and 20:30 p.m.