## 3 Results
### GC-MS Profiling of the Supercritical CO\({}_{2}\) Extract  
Supercritical fluid extraction was performed on the moss cake with the aim of identifying non-polar components that may have been excluded from the aqueous extraction in the previous section. In addition to components previously identified in the earlier extract, such as C16 or C18, which were found in higher concentrations in this extraction, the predominant compound discovered was ceruchinol. Other Kaurane derivates like Kaur-15-ene and Kaur-16-ene were also detected in the CO\({}_{2}\) extract. Furthermore, potential substrates or products of suberin polymerization [42] were detected in the extract, including derivatives of cinnamic acid, long-chain aldehydes, and secondary alcohols. This finding provides additional support for our assumption regarding the composition of the protonemata and gametophores of _P. patens_, reinforcing the notion that the moss may exhibit a complex makeup involving these identified compounds. The GC-MS analysis of the supercritical CO\({}_{2}\) extract resulted in the signals shown in Figure 6 and Supplementary Figure S1. Comparison of the sample with internal standards showed that ceruchinol was present as a major peak and eluted at a retention time of 71.21 min. Other molecules were as well profiled, as shown in Table 1, particularly long-alkyl chain lipidic constituents and terpenoids, among which significant kaurene diterpenes. \begin{table}
\begin{tabular}{c c c c c c} \hline
**PK** & **RT** & **Library/ID** & **CAS** & **Qual** & **Area** & **Confirmed?** \\ \hline
**1** & 7.39 & Acetic acid & 64-19-7 & 86 & 1.30 \(\times\) 10\({}^{8}\) & Yes \\ \hline
**2** & 12.24 & HEXANAL & 66-25-1 & 93 & 30,055,867 & Yes \\ \hline
**3** & 21.03 & 2-Octen-1-ol, (Z)- & 26001-58-1 & 93 & 25,079,272 & Yes \\ \hline
**4** & 24.4 & 9.14 Benzene acetaldehyde & 122-78-1 & 87 & 14,788,190 & Yes \\ \hline
**5** & 32.15 & 15.77 Dodecane & 112-40-3 & 95 & 56,976,919 & Yes \\ \hline
**6** & 33.77 & 2-Cyclopenten-1-one, 2-methyl- & 1120-73-6 & 89 & 5,702,674 & Yes \\ \hline
**7** & 36.47 & 19.71 Thymol & 89-83-8 & 95 & 72,963,027 & Yes \\ \hline
**8** & 36.86 & 20.14 Carvacrol & 499-75-2 & 95 & 37,180,865 & Yes \\ \hline
**9** & 41.2 & 24.61 Tetradecane (C14) & 629-59-4 & 97 & 1.14 \(\times\) 10\({}^{8}\) & Yes \\ \hline
**10** & 45.57 & Butylated Hydroxytoluene & 128-37-0 & 97 & 7,402,971 & Yes \\ \hline
**11** & 49.25 & 32.89 Hexadecane (C16) & 544-76-3 & 98 & 63,206,613 & Yes \\ \hline
**12** & 50.62 & Benzene, 1-methyl-4-[(1- & & & \\  & & & methylethylidene)cyclopropyl]- & 24578-28-7 & 71 & 9,609,670 & Yes \\ \hline
**13** & 51.14 & 34.73 Amyl cinnamaldehyde\(<\)Z-\(>\) & 101365-33-7 & 99 & 6,484,050 & Yes \\ \hline
**14** & 52.62 & 1-Methyl-5-nitro-1H-benzoinidazol-2 ol & 66108-85-8 & 65 & 9,853,674 & Yes \\ \hline
**15** & 54.78 & 39.42 Cinnamaldehyde\(<\)2-hexyl-(Z)-\(>\) & 364364-06-7 & 99 & 17,036,726 & Yes \\ \hline
**16** & 55.64 & 38.90 Benzyl benzoate & 120-51-4 & 95 & 17,676,783 & Yes \\ \hline
**17** & 56.49 & 40.40 Octadecane (C18) & 593-45-3 & 98 & 28,462,303 & Yes \\ \hline \end{tabular}
\end{table} 
Table 1: Molecules detected at the Supercritical CO\({}_{2}\) extract. Ceruchinol appears as Kauran-16-ol at 71.21 min. PK: Peak, RT: Retention time, CAS numbers, Area: surface under peak. Figure 6: GC-MS profile of the supercritical CO\({}_{2}\) extract at 1 mg/mL in EtOH. Total ion current obtained. Ceruchinol was detected at Tr = 71.21 min (red arrow) and corresponds to the major peak. At Supplementary Figure S1 one could see the same graphic but without any zoom in order to reveal the importance of the ceruchinol peak. Like ceruchinol, Kaurane diterpenes represent a class of organic compounds characterized by a distinctive tetracyclic diterpene skeleton. Structurally, they consist of four fused rings, with variations in functional groups and side-chain modifications contributing to their diverse bioactivities.