# Immunomodulatory Effects of Pentoxifylline: Profiling Data Based on RAW 264.7 Cellular Signaling
## 3 Results
\begin{table}
\begin{tabular}{c c c} \hline \hline
**Signaling Proteins** & **No.** & **Antibodies** \\ \hline Cellular proliferation & 10 & Ki-67 *, PCNA *, CDK4 *, PLK4 *, MPM2 *, p14 *, p16 *, p21 *, p27 *, cyclin D2 * \\ cMyc/MAX/MAD signaling & 3 & cMyc *, MAX *, MAD * \\ p53/Rb/E2F signaling & 4 (2) & p53, Rb-1 *, E2F-1 *, MDM2 *, CDK4 *, p21 * \\ Epigenetic modification & 6 & DMAP1, histone H1 *, KDM4D *, HDAC-10 *, MBD4, DNMT \\ Protein translation signaling & 3 & DOHH *, DHS *, eIF5A-1 \\ RAS signaling & 7 (3) & NRAS *, KRAS *, HRAS, Rab, JNK-1 *, ERK-1 *, MEKK, (pAKT1/2/3 *, \\ Growth factor signaling & 9 & TGF-81 *, IGF-1 *, HER1 *, HER2 *, ER9 *, FGF-1, FGF-2, Met, CTGF \\ NFkB signaling & 9 (3) & NFkB *, IKK *, NFATS *, PGC-1\(\alpha\), GADD45 *, GADD153 *, mTOR *, p38 *, \\ MDR & (ERK-1 *, pAKT1/2/3 *, TNF\(\alpha\) *) & CD3 *, CD4D20 *, CD28 *, CD31 *, CD68 *, cathepsin K * \\  & TNF\(\alpha\) *, IL-1 *, IL-10 *, IL-2 *, COX2 *, lysozyme *, MCSF *, MMP-1 *, \\ Inflammatory signaling & 20 & MMP-2 *, MMP-3 *, MMP-9 *, MMP-10 *, MMP-12, MCP-1, LTA4H, CXCR4 \\  & TLK, hepcidin, CRP * 1 \\ Cell protection & 8 (3) & HSP-70 *, HSP-90 *, AP-1 *, SP-1 *, SP-3 *, p38 *, PKC *, pAKT1/2/3 *, (p38, JNK-1, TERT) \\ Cellular differentiation & 6 (4) & PLC-92, TGase-2, HKKII *, Jagged-2, Notch, GLI-1, (PKC, AP-1, SP-1, SP-3) \\ Antioxidant-related proteins & 5 & SOD-1 *, GST *, LC3 *, AMPK *, NCS-1, \\ p53-mediated cellular apoptosis & 6 (1) & p53 *, MDM2 *, BAK *, PAFA-1 *, caspase * * (PARP) \\ FAS-mediated cellular apoptosis & 6 & FASL *, FAS *, FADD *, FLIP *, caspase *, PARP * \\ Oncogenic proteins & 3 (3) & 14-3-3 *, TERT *, YAP, (pAKT1/2/3, MBD4, mTOR) \\  & HIF *, VEGF-A *, VEGF-C *, VCAM, angiogenin *, CMG2 *, vWF *, ET-1 \\  & & *(CD31, MMP-2, MMP-10, FGF-2) \\ Osteogenesis-related proteins & 9 (2) & RANKL, OPG *, osteonectin, osteonectin, st osteocolcin, RUNX2, ALP *, osterix *, BMP-2 * (cathepsin K, HSP-90) \\ Control cytoplasmic proteins & 3 & \(\alpha\)-tubulin *, p-actin *, GAPDH * \\ Total & 132 (25) & \\ \hline \hline \end{tabular}
* Santa Cruz Biotechnology, USA;
* DAKO, Denmark;
* Neomarkers, CA, USA;
* ZYMED, CA, USA;
* Abcam, Cambridge, UK. Abbreviations: ALP: alkaline phosphatase, AMPK: AMP-activated protein kinase, APAF-1: apoptotic protease-activating factor 1, AP-1: activating protein-1, BAK: BCL2 antagonist/killer, BAK: BCL2 associated X, BMP-2: bone morphogenetic protein-2, CASP 3: caspase 3, CASP 8: caspase 9, CASP 9: caspase 9, C-CASP 9: cleaved- caspase 9, CD3: cluster of differentiation 3, CDK4: cyclin dependent kinase *, CEA: carcinomaembryonic antigen, cMyc: V-myc pseudocolcinosis viral oncogene homolog, CMG2: capillary morphogenesis protein 2, COX2: cyclooxygenase-2, CRP-1: C-reactive protein-1, CXCR4: C-X-C chemokine receptor type 4, DMAP1: DNA methyltransferase 1 associated protein, DNMT: DNA (cytosine-5)-methyltransferase, DOHH: deoxytyping hydroxylase, DHS: deoxytyping synthase, EZF-1: transcription factor, eIF5A-1: eukaryotic translation initiation factor 5A-1, ER9: estrogen receptor beta, ERK: extracellular signal-regulated protein kinases, ET-1: endothelium-1, FAS: CD95/Apo1, FASI: FAS ligand, FADD: FAS associated via death domain, FGF-1: fibroblast growth factor-1, FLIP: FLICE-like inhibitory protein, vascular endothelial growth factor receptor 3 precursor (FLT4), GADD45: growth arrest and DNA-damage-inducible 45, ATDP1: glyceraldehyde 3-phosphate dehydrogenase, GST: glutathione *, HDAC-10: histone deacetylase-10, HIF: hypoxic inducible factor-1a, Histone H1, HER1: human epidermal growth factor receptor 1, JHERT: human telomerase reverse transcriptase, HSP-70: heat shock protein-70, HSP-90: heat shock protein-90, HKKII: hexokinase II, IKK: kappa kinase, KF-1, IL-1: interleukin-1, JNK-1: Jun 8-terminal protein kinase, KDM4D: lysine-Specific Demethylase 4D, KRAS: V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, LC3: microtubule-associated protein 1A/1B-light chain 3, ITAH: Leukotine 4A hydrolase, MAX: myc-associated factor X, MBD4: methyl-CPG-binding domain protein 4, MCP-1: monocyte chemoattractant protein 1, M-CSF: macrophage colony-stimulating factor, MDM2: mouse double minute 2 homolog, MEKK: MAP kinase kinase kinase, MPM2: mitotic protein monoclonal 2, MDR: Monoclonal Anti-P-Glycoprotein, Met tyrosine-protein kinase, MRP-1: matrix metalloproteinase-1, MMP-2: matrix metalloproteinase-2, mTOR: mammalian target of rapamycin, NFATS nuclear factor of activated T-cells, NFkB: nuclear factor kappa-light-chain- enhancer of activated 6 B cells, NOS-1: NRAS: neuroblastoma RAS Viral oncogene homolog, OPG: osteoproteger, OPN: osteonotin, OSX: osterix, pAKT: v-akt murine thymroma viral oncogene homolog, P-Akt1/2/3 phosphorylated (p-Akt, Thr 308), PARP: poly-ADPribose polymerase, PCNA: proliferation cell nuclear antigen, PGC-1a: peroxisome Prolifter-Activated Receptor-v-Coactivator 1a, PKC: protein kinase, PLC-82: 1-phosphotidylinositol-4,5-bisphosphate phosphodiesterase beta-2, Rb-1: retinoblastoma-1, RANKL: receptor activator of nuclear factor kappa-B ligand, RUNX2: Runt-related transcription factor 2, SOD-1: superoxide dismutase-1, SP-1: specificity protein 1, SP-3: specificity protein 3, TGase-2: transglutaminase-2, TGF-81: transforming growth factor-81, TLR3: Toll like receptor 3, TNF-ac: tumor necrosis factor-\(\alpha\), \(\beta\)-actin, 14-3-3, VEGF vascular endothelial growth factor, vWF: von Willebrand factor. The number of antibodies overlapped: ().  
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