## 2 Materials and Methods
Note: IS—ischemic stroke; NIS—neonatal ischemic stroke; PIS—pediatric IS; HS—hemorrhagic stroke; VST—venous sinus thrombosis; CCT—acute traumatic brain injury; LCIP—lack of informed consent from parents; ECG—electrocardiogram; EcoCG—transthoracic echocardiography; DP—Doppler or duplex Doppler of cerebral vessels; EEG—electroencephalography; NSG—neurosonography; CT—computed tomography; MRI—magnetic resonance imaging; MRA—magnetic resonance angiography; MRV—magnetic resonance venography; ADC—conventional digital angiography; IL——interleukin-6; CNTF—cliary neurotrophic factor; S100B—S100B protein; CD105—CD105 (endolin); VEGF—vascular endothelial growth factor; APAs——antiphospholipid antibodies; CN—neurological consequences. paraclinical manifestations of AIS and AIPS depending on the involved determinants (perinatal and postnatal) and the child's age. In the third stage, the neurological complication prognosis of AIS patients was assessed. Mathematical analysis methods were used to evaluate the relationship between the determinants involved in AIS, the child's age, the clinical features at the time of diagnosis, immunoenzymatic changes, and the risk of neurological complications. Predictive methods for the prophylaxis of AIS and neurological complications in children were proposed. The fourth stage involved developing an algorithm for managing women/families at risk of giving birth to a child with AIS and an algorithm for diagnosing and managing newborns and children with AIS. A National Clinical Protocol for Pediatric Stroke was also developed for the prophylaxis, early diagnosis, and definitive treatment of stroke in pediatric patients for doctors and the population of the Republic of Moldova. In the context of this study, the following inclusion/exclusion criteria served as conceptual and methodological benchmarks for patient selection. The inclusion criteria for patients in the study were as follows:  
1. Children with acute ischemic stroke of various types. 2. Newborn children (0-28 days old). 3. Children aged between 28 days and 18 years. 4. Pregnant women. 5. Informed consent from the parents or caregivers. The exclusion criteria for the study were as follows:  
1. Children with hemorrhagic stroke. 2. Venous sinus thrombosis. 3. Children older than 18 years. 4. Children with acute traumatic brain injury. 5. Premature children. 6. A lack of informed consent from the parents or caregivers. A retrospective study involving the review and analysis of 402 medical records of children diagnosed with acute ischemic stroke (AIS) between 2010 and 2016 and a prospective, non-randomized study of 108 children who experienced AIS between 2017 and 2020 were carried out. In the prospective study, all 108 children with confirmed AIS were evaluated according to Amiel-Tison and Gosselin neurological examination (designed for children of various age groups). Additionally, children older than three months in the study group were prospectively evaluated using the pedNIHSS scale, adapted for children. Out of the 108 children examined, 82 (75.9%; 95% CI [71.79-80.01]) exhibited motor deficits, 45 (41.7%; 95% CI [36.96-46.44]) had seizures, and 19 (17.6%; 95% CI [13.94-21.26]) experienced consciousness disturbances. Furthermore, 87 (80.6%; 95% CI [76.79-84.41]) presented with unilateral AIS, and 77 (71.3%; 95% CI [66.95-75.65]) were found to have signs of damage in the territory of the middle and anterior cerebral artery circulation. Additionally, in this stage of the study, we evaluated a group of pregnant women, considering that developing predictive methods for AIS in children could only be feasible within the context of a medical-genetic consultation. The medical-genetic consultations for the pregnant women in this study were conducted in the Medical Genetics Department of the MHCI IMC and in the Consultative Department of MHCI HCM nr. 1 in Chisinau.