## 3 Results and Discussion
### Alkaline Protease and Detergent Auxiliary Washing Effect Test  
The effect of detergents mixed with different detergent auxiliaries and alkaline protease ratios to clean protein-soiled cloth and blood-dirty cloth was tested (Tables 2 and 3). The evaluation criterion is whether the decontamination effect of the mixed detergent is better than that of only the alkaline protease detergent. Better washing surfactants include sodium alginate, FMEE, SNS-80, hyaluronic acid, APG [32], SOE, MES, and tea saponin. Water  
Figure 5: Deposition effect after the blood is decomposed by proteases. (**A**) Alkaline protease. (**B**) Keratinase. (**C**) Trypsin. (i) Reaction 10 min. (ii) Reaction decomposition 20 min. (iii) Reaction 30 min. (iv) Reaction 40 min. (v) Reaction 50 min. (vi) No added protease. softening agents with a better washing effect are 4A zeolite [33; 34], sodium gluconate, sodium tartrate, and sodium laurate. The ones that inhibit the effect of alkaline protease are SKS-6, SiO\({}_{2}\), and EDTA. Experimental results show that in the washing process of using alkaline protease detergent, whether it is for protein or bloodstream, it can be mixed with water-softening agent sodium gluconate, surfactant sodium alginate, FMEE, hyaluronic acid, APG, MES, and tea saponin [35; 36]. In addition, bloodstain washing can be mixed with anti-deposition agent HPMC, sodium hydroxyethyl cellulose [37], sodium polyacrylate, and PVA. SOE, SiO\({}_{2}\), CMC, disodium maleate, PASP, PEG, EDTA, and EDTA exert inhibitory effects on the protein decomposing effect of alkaline protease. \begin{table}
\begin{tabular}{c c c c} \hline \hline \multirow{2}{*}{**Washing Performance**} & \multicolumn{3}{c}{**Reflectivity (\%)**} \\ \cline{3-4}  & & **Protein-Fouling Cloth** & **Blood-Dirty Cloth** \\ \hline \multirow{8}{*}{Surfactant} & Before washing & 13.23 \(\pm\) 1.12 & 10.78 \(\pm\) 1.01 \\  & Basal detergent & 20.90 \(\pm\) 0.56 & 14.93 \(\pm\) 1.30 \\  & Alkaline protease & 25.60 \(\pm\) 0.59 & 25.20 \(\pm\) 1.39 \\ \hline \multirow{8}{*}{Surfactant} & APG & 27.03 \(\pm\) 0.46 & 24.50 \(\pm\) 1.67 \\  & SOE & 26.90 \(\pm\) 1.70 & 21.98 \(\pm\) 2.12 \\  & MES & 26.90 \(\pm\) 0.59 & 25.18 \(\pm\) 0.86 \\ \cline{1-1}  & SNS-80 & 28.55 \(\pm\) 1.10 & 23.98 \(\pm\) 2.32 \\ \cline{1-1}  & FMEE & 29.28 \(\pm\) 2.00 & 30.15 \(\pm\) 1.90 \\ \cline{1-1}  & Sodium alginate & 28.58 \(\pm\) 1.40 & 27.00 \(\pm\) 0.76 \\ \cline{1-1}  & Hyaluronic acid & 25.40 \(\pm\) 1.26 & 18.35 \(\pm\) 1.94 \\ \cline{1-1}  & Tea saponin & 23.93 \(\pm\) 1.52 & 24.48 \(\pm\) 1.29 \\ \hline \multirow{8}{*}{Water-softening agent} & EDTA & 18.86 \(\pm\) 2.01 & 12.56 \(\pm\) 1.31 \\  & SiO\({}_{2}\) & 21.34 \(\pm\) 0.28 & 12.64 \(\pm\) 1.11 \\ \cline{1-1}  & SKS-6 & 22.43 \(\pm\) 1.25 & 14.79 \(\pm\) 1.01 \\ \cline{1-1}  & Sodium Tartrate & 24.95 \(\pm\) 0.68 & 14.64 \(\pm\) 1.36 \\ \cline{1-1}  & Sodium laurate & 24.20 \(\pm\) 1.39 & 16.65 \(\pm\) 1.38 \\ \cline{1-1}  & Sodium Gluconate & 25.58 \(\pm\) 1.01 & 16.69 \(\pm\) 1.48 \\ \cline{1-1}  & 4A zeolite & 26.71 \(\pm\) 1.57 & 13.80 \(\pm\) 1.28 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Washing effect of different surfactants and water-softening agents mixed with alkaline protease on dirty cloth.