# Complete Genome Report of a Hydrocarbon-Degrading _Sphingobium yanoikuyae_ S72
## 3 Results
The deep sequence analysis of S72 showed that it possesses 37 genes in different COG categories which are associated with the degradation of xenobiotics and polycyclic aromatic hydrocarbons. These genes include 2 pyrone-4-6-decarboxylase hydroylase (A6768_17510, COG R) and 4 carboxy-2-hydroxy muonic semialdehyde dehydrogenase (A6768_17470, COG R). Others include S-(hydroxymethyl) glutathione dehydrogenase/alcohol dehydrogenase (A6768_05785, COG R) that is associated with the degradation of naphthalene and chloroalkane). Alcohol dehydrogenase (cytochrome c) (A6768_00755, COG G), aldehyde dehydrogenase (NAD+) (A6768_10925, COG E), aldo/keto oxidoreductase-tase (A6768_11370, COG R), phenylacetaldehyde dehydrogenase (A6768_11850, COG C) known to participate in the degradation of fluorobenzene toluene and naphthalene are also present in S72 [3,8]. Other hydrocarbon degrading genes identified in S72 include propranolol preferring alcohol dehydrogenase (A6768_17370, EC:1.1.1.1), carboxymethylenebutenolidase (A6768_23915), catechol 1,2-dioxygenase (A6768_16795), muocane cycloisomerase (A6768_16805), aryl-alcohol dehydrogenase (A6768_11850), 2-keto-4-pentenoate hydratase (A6768_14055), oxalocrotonate tautomerase (A6768_04785) benzoate 1,2-dioxygenase alpha subunit (A6768_16790), benzoate/toluate 1,2-dioxygenase beta subunit (Ben B), dihydroxy cyclohexadiene carboxylate dehydrogenase (Ben D). Similarly, a gene encoding the lactolyglutathione lyase was found in S72. The lactolyglutathione lyase enzyme is known to be \begin{table}
\begin{tabular}{c c c c c c c} \hline
**COG List** & \multicolumn{3}{c}{**Gene Abundance**} & **StdErr Other** & **FishersTest Pval** & **FishersTest adjPval** \\ \hline \multirow{4}{*}{**S72 (\%)**} & **Mean COG in** & \multirow{2}{*}{**Differences**} & \multirow{4}{*}{**} & \multirow{4}{*}{**} \\  & & **other\_sphingobium** & & & & \\  & & (_n = 8_) (\%) & & & & \\ \hline Carbohydrate transport and & & & & & & \\ metabolism (G) & 6.20 & 5.66 & 0.54 & 0.11 & 0.16 & 0.39 \\ Coenzyme transport and & & 4.95 & 4.53 & 0.42 & 0.12 & 0.20 & 0.44 \\ metabolism (H) & & 1.50 & 1.70 & \(-\)0.20 & 0.04 & 0.40 & 0.74 \\ Cell motility (N) & 2.26 & 2.52 & \(-\)0.26 & 0.06 & 0.38 & 0.74 \\ Defense mechanisms (V) & 6.35 & 6.67 & \(-\)0.32 & 0.08 & 0.46 & 0.78 \\ Function unknown (S) & 4.65 & 4.85 & \(-\)0.20 & 0.15 & 0.51 & 0.80 \\ mechanisms (T) & & 6.61 & 6.43 & 0.18 & 0.08 & 0.65 & 0.85 \\ Amino acid transport and & & 1.73 & 1.64 & 0.09 & 0.07 & 0.63 & 0.85 \\ metabolism (F) & & 7.80 & 7.60 & 0.20 & 0.08 & 0.71 & 0.85 \\ General function prediction & & 9.93 & 9.67 & 0.27 & 0.22 & 0.73 & 0.85 \\ Extracellular structures (W) & & 0.51 & 0.56 & \(-\)0.06 & 0.02 & 0.71 & 0.85 \\ Cell & & & & & & \\ wall/membrane/envelope & & 6.58 & 6.47 & 0.11 & 0.11 & 0.80 & 0.85 \\ biogenesis (M) & & & & & & \\ Posttranslational & & & & & & \\ modification, protein & 4.27 & 4.20 & 0.07 & 0.10 & 0.81 & 0.85 \\ turnover, chaperones (O) & & & & & & \\ Translation, ribosomal & & 4.73 & 4.74 & \(-\)0.02 & 0.19 & 0.89 & 0.89 \\ structure, and biogenesis (I) & & & & & & \\ RNA processing and & & 0.00 & 0.00 & 0.00 & 1.00 & 1.00 \\ modification (A) & & & & & & \\ Chromatin structure and & & & & & & \\ dynamics (B) & & 0.03 & 0.02 & 0.00 & 0.00 & 1.00 & 1.00 \\ \hline \end{tabular}
\end{table} 
Table 3: _Cont._actively involved in the cleavage of aromatic bond in many aromatic hydrocarbons [38]. Among the observed genes in S72 are genes essential for the degradation of toluene. The genes include four genes encoding 3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35), one gene for aryl-alcohol dehydrogenase (EC:1.1.1.90), and a gene for catechol 1,2-dioxygenase (EC:1.13.11.1). Others include two genes encoding the enzyme oxidoreductase (EC:1.14.13) which often act on paired donors, with incorporation or reduction of molecular oxygen.