## 3 Results
### Influence of the Storage Temperature on Oxidant Stability and Antifungal Activity  
The influence of the storage temperature is showed in Figure 3. Storage at 4 \({}^{\circ}\)C noticeably increased the shelf-life of the oxidant, whatever the substrate dose and the initial oxidant concentration. The solutions prepared with double-dose substrate concentrations contained higher initial oxidant doses, but also underwent a higher degradation rate. Full-dose solutions prepared with high mineralized waters (Medium High and High) and double-dose solutions prepared with low mineralized waters (Low and Medium low) showed similar initial oxidant doses of around 500 uM. However, the shelf-life at 25 \({}^{\circ}\)C was around 90 days for the full dose and 25 days for the double dose. At 4 \({}^{\circ}\)C, it was more than 360 days for the full dose and 120 days for the double dose. The biological activity of the oxidant was clearly related to its dose (Tables 4-6), which implies that the rate of oxidant degradation affects biological activity duration. The remanence of biological activity was influenced by the storage temperature and the initial oxidant dose. Cold storage of the oxidant and reduced amounts of substrates (half and full doses) prolonged the biological activity of the oxidant. The storage temperature also seemed to affect the minimum inhibitory dose, with enhanced efficiency of the oxidants kept at 4 \({}^{\circ}\)C. At 25 \({}^{\circ}\)C, the oxidant dose necessary for biological activity was around 205 uM for the half dose, 157 uM for the full dose, and 219 uM for the double dose. At 4 \({}^{\circ}\)C, the oxidant dose necessary for biological activity was around 150 uM for the half dose, 111 uM for the full dose, and 172 uM for the double dose. \begin{table}
\begin{tabular}{c c c c c} \hline \hline  & \multicolumn{4}{c}{**Water Mineralization**} \\ \cline{2-5}  & **Low** & **Medium Low** & **Medium High** & **High** \\ \hline
**Substrate Doses** & & **Oxidant Concentration (μM)** & \\ \hline
**Half-dose** & 291 \(\pm\) 20 & 254 \(\pm\) 44 & 235 \(\pm\) 32 & 256 \(\pm\) 16 \\
**Full-dose** & 383 \(\pm\) 25 & 407 \(\pm\) 17 & 503 \(\pm\) 33 & 538 \(\pm\) 42 \\
**Double-dose** & 546 \(\pm\) 24 & 563 \(\pm\) 18 & 785 \(\pm\) 29 & 974 \(\pm\) 49 \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Oxidant concentration measured as \(-\)NH\({}_{2}\) oxidation (μM) after LPO oxidoreduction of 2.7 mM KI + 0.6 mM KSCN + 3.3 mM H\({}_{2}\)O\({}_{2}\) (half-dose); 5.4 mM KI + 1.2 mM KSCN + 6.6 mM H\({}_{2}\)O\({}_{2}\) (full dose) and 10.8 mM KI + 2.4 mM KSCN + 13.2 mM H\({}_{2}\)O\({}_{2}\) (double-dose) in low- to high-mineralized waters. Means and s.d. of two independent experiments are given. \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline  & & \multicolumn{4}{c}{**Storage Duration (Days)**} \\ \cline{3-6}  & & **0** & **30** & **90** & **240** & **360** \\ \hline \multirow{2}{*}{**Temp.**} & **Water** & \multicolumn{4}{c}{**P. _expansum_ Inhibition (\%)**} \\  & **Mineralization** & & & & \\ \hline \multirow{4}{*}{**25 \({}^{\circ}\)C**} & **Low** & \(91\pm 5\) b ** & \(81\pm 4\) a  & \(26\pm 4\) c  & \(0\pm 6\) ns  & \(4\pm 2\) ns  \\  & **Medium Low** & \(88\pm 7\) b  & \(24\pm 10\) ns  & \(0\pm 2\) ns  & \(0\pm 2\) ns  & \(0\pm 4\) ns  \\  & **Medium High** & \(92\pm 2\) b  & \(0\pm 1\) ns  & \(5\pm 5\) ns  & \(0\pm 4\) ns  & \(7\pm 7\) ns  \\  & **High** & \(89\pm 3\) b  & \(0\pm 1\) ns  & \(3\pm 6\) ns  & \(0\pm 5\) ns  \\ \hline \multirow{3}{*}{**4 \({}^{\circ}\)C**} & **Low** & \(100\pm 0\) a  & \(73\pm 8\) a  & \(95\pm 3\) a  & \(95\pm 3\) a  & \(95\pm 1\) a  \\  & **Medium Low** & \(99\pm 1\) a  & \(89\pm 5\) a  & \(91\pm 2\) a  & \(96\pm 4\) a  & \(92\pm \) a  \\  & **Medium High** & \(100\pm 0\) a  & \(73\pm 7\) a  & \(53\pm 3\) b  & \(61\pm \) c  & \(90\pm \) a  \\  & **High** & \(100\pm 0\) a  & \(75\pm 4\) a  & \(63\pm 11\) b  & \(85\pm \) b  & \(90\pm \) a  \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Percentage of inhibition of _P.