## 3 Results
### Quantitative Analysis of Antibiotic Resistance Genes and Taxonomic Genes
#### 3.2.3 Quantitative Occurrence of _uid_A Genes  
The average concentration of the taxonomic gene of _E. coli_ ranged from 9.97 \(\times\) 10\({}^{-3}\) to 5.63 \(\times\) 10\({}^{-2}\) gene copies/16S _r_RNA gene copies for the DNA from the total population of bacteria isolated on TSA medium, and from 1.50 \(\times\) 10\({}^{-2}\) to 4.29 \(\times\) 10\({}^{-1}\) gene copies/16S _r_RNA gene copies for the DNA derived from the population of _E. coli_ isolated on the mFc medium. The concentrations of genes _uid_A in the DNA of the total bacterial population were positively correlated with the concentration of genes _tet_A and _bla_TEM, while concentrations of genes _uid_A in the DNA of _E. coli_ population were only positively correlated with gene _bla_TEM. The greatest reduction in the concentration of _E. coli_, based on the taxonomic gene, was observed in WWTP No. XII, group D (80%) in the DNA of the total bacterial population and in WWTP No. V, group B (40%) for the DNA of _E. coli_ population. The relative concentration of gene _uid_A following the wastewater treatment process increased for the DNA  
\begin{table}
\begin{tabular}{c c c c c c c c} \hline
**Sewage Treatment Technology** & **Treatment** & _tet_A** & _tet_M** & _bla_TEM** & _bla_OXA** & _bla_SHV** & _uid_A** \\ \cline{2-9} \multirow{3}{*}{A. WWTPs with A\({}_{2}\)O system} & 1 & * & 93.66 & 44.30 & ** & 17.50 & * \\ \cline{2-9}  & II & ** & ** & ** & * & 64.30 & 53.95 & 14.67 \\ \hline \multirow{3}{*}{B. WWTPs with mechanical-biological system} & III & * & 99.66 & * & 100 & * & * \\ \cline{2-9}  & IV & * & * & * & * & * & * \\ \cline{2-9}  & V & * & * & * & * & 7.63 & 40.10 \\ \cline{2-9}  & VI & ** & * & * & ** & 43.52 & 10.82 \\ \cline{2-9}  & VII & 100 & 99.997 & 14.50 & * & 90.47 & 33.22 \\ \hline \multirow{3}{*}{C. WWTPs with Sequencing Batch Reactors (SBR)} & VIII & 100 & 86.13 & 21.88 & ** & 84.69 & 27.77 \\ \cline{2-9}  & IX & 60.98 & 99.76 & 80.98 & ** & 88.40 & 20.75 \\ \cline{2-9}  & X & 100 & 68.71 & * & 85.93 & 26.06 & * \\ \hline \multirow{3}{*}{D. WWTPs with mechanical-biological system with elevated removal of nutrients} & XI & 100 & * & * & ** & * & * \\ \cline{1-1} \cline{2-9}  & XII & 66.62 & 80.96 & * & * & * & * \\ \cline{1-1} \cline{2-9}  & XIII & 98.21 & 19.55 & 97.30 & ** & * & 15.52 \\ \hline \end{tabular}  
*—increase in gene concentration, **—**–no gene presence, WWTP— wastewater treatment plant.  
\end{table}
Table 4: Gene concentration reduction in _E. coli_ populations.  
\begin{table}
\begin{tabular}{c c c c c c c c c} \hline  & **mFc/** & **mFc-AMO/** & **mFc-CIV/** & **mFc-OAV** & **mFc-DOX/** & _tet_A** & _tet_M** & _bla_TRA** & _bla_OXA** & _bla_SHV** & _uid_A** & **HRT** & **TSS** & **BOD** \\ \cline{2-9}  & **1 mL** & **1 mL** & **1 mL** & **1 mL** & **1 mL** & **1 mL** & & & & \\ \hline mFc-AMO/ & **0.93** & & & & & & & & & \\ mFc-C/C/ & & **0.66** & **0.74** & & & & & & & \\ mFc-OX/ & & **0.81** & **0.83** & **0.39** & & & & & & \\ mFc-DOX/ & & **0.66** & **0.69** & **0.65** & **0.73** & & & & & & \\ \cline{2-9} \multirow{3}{*}{IntrA} & 0.24 & 0.13 & 0.13 & 0.14 & 0.07 & 0.05 & -0.02 & **0.56** & & & & \\ mFc-DOX/ & & 0.16 & 0.07 & -0.11 & 0.09 & 0.13 & **0.30** & 0.24 & & & & \\ mFc-DOX/ & & & & & & & & & & \\ \cline{2-9} \multirow{3}{*}{IntrA} & -0.09 & -0.02 & -0.12 & 0.02 & -0.03 & -0.12 & **0.25** & & & & & \\ mFc-DOX/ & & & & & & & & & & \\ mFc-DOX/ & & & & & & & & & & & \\ \cline{2-9} \multirow{3}{*}{IntrA} & -0.04 & -0.12 & -0.32 & -0.15 & -0.06 & 0.11 & 0.06 & **0.32** & -0.04 & 0.02 & & & \\ HRT & -0.05 & -0.08 & 0.08 & -0.11 & -0.23 & 0.03 & 0.14 & -**0.61** & -0.38 & -0.10 & -0.12 & & \\ TSS & -0.01 & 0.06 & **0.46** & 0.14 & 0.23 & -0.03 & -0.31 & -0.05 & -0.12 & -0.19 & -0.01 & -0.07 & \\ BOD & **-0.63** & **-0.63** & **-0.69** & **-0.54** & **-0.43** & -0.31 & -0.35 & -0.11 & -0.13 & -0.23 & 0.05 & 0.04 & -0.15 obtained from _E. coli_ population in six wastewater treatment plants, while in the DNA from the total bacterial population, from five WWTPs.