## 4 Conclusions  
This study has highlighted numerous newly identified and tentatively identified volatile compounds present in the headspace of'salad' rocket, wasabi, horseradish, and watercress. Many of these appear to contribute strongly to the aroma profiles of each respective crop. We have also found 46 aroma traits present in the headspace of the samples that have no association with the identified compounds. This suggests that there are many as-yet undiscovered odour-active compounds present within Brassicales headspace. Our data also highlight the need for more detailed studies on the volatilome of Brassicales species, and that the sole focus should not be upon GSL hydrolysis products. While accounting for a large proportion of the respective volatile profiles and odour active components of species, we have identified numerous instances where non-GSL derived compounds have odour intensities greater than those that are GSL-derived, such as 1-penten-3-ol (**25**), phenylacetaldehyde (**52**), 2-_sec_-butyl-3-methoxypyrazine (**106**), and \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Precursor** & **Glucosinolate Hydrolysis** & \multicolumn{3}{c}{**Glucosinolate Observed?**} & \multicolumn{3}{c}{**Hydrolysis Product Observed?**} \\
**Glucosinolate** & **Product (Compound No.)** & **Salad Rocket** & **Wasabi** & **Waterscens** & **Salad Rocket** & **Wasabi** & **Waterscens** \\ \hline \multirow{5}{*}{sinigrin} & 3-butenemitrile (**74**)  & \(\surd\) & \(\surd\) & \(\surd\) & x & \(\surd\) & x \\  & allyl thiocyanate (**4**)  & \(\surd\) & \(\surd\) & \(\surd\) & x & \(\surd\) & x \\  & allyl ITC (**5**/**6**)  & \(\surd\) & \(\surd\) & \(\surd\) & x & \(\surd\) & x \\  & cyclopropate ITC (**7**)  & \(\surd\) & \(\surd\) & \(\surd\) & x & x \\  & thiraaceronitrile (**77**)  & \(\surd\) & \(\surd\) & \(\surd\) & x & x \\ glucocoppararin & methyl thiocyanate (**2**)  & x & x & \(\surd\) & \(\surd\) & x \\ glucoputrianium \(\lx@sectionsign\) & isopropyl ITC (**3**)  & \(\surd\) & \(\surd\) & x & \(\surd\) \\ glucocococochearin \(\lx@sectionsign\) & sc-butyl ITC (**9**)  & x & \(\surd\) & x & \(\surd\) & x \\
4-methylpently GSL & \(\lx@sectionsign\) & 5-methylhexanonitrile (**75**)  & \(\surd\) & x & \(\surd\) & \(\surd\) & x \\  & 1-isothiocyanate- & & & & & \\  & 4-methylpentane (**16**)  & \(\surd\) & x & \(\surd\) & \(\surd\) & x \\  & 4-methylpently GSL & \(\lx@sectionsign\) & & & & & \\  & 4-methylpentane (**16**)  & x & \(\surd\) & \(\surd\) & \(\surd\) & x \\  & 4-methylpentane (**16**)  & x & \(\surd\) & \(\surd\) & \(\surd\) & x \\  & 4-methylpentane (**16**)  & x & \(\surd\) & \(\surd\) & \(\surd\) & x \\  & 6-heptenemitrile (**76**)  & \(\surd\) & \(\surd\) & x & \(\surd\) & x \\  & glucocapurin & 3-butternly ITC (**11**)  & \(\surd\) & \(\surd\) & \(\surd\) & \(\surd\) & x \\ butyl GSL & butyl ITC (**12**)  & \(\surd\) & x & \(\surd\) & \(\surd\) & \(\surd\) & x \\  & 3-methylbutyl GSL & isoamyl ITC (**13**)  & x & x & \(\surd\) & \(\surd\) & \(\surd\) \\  & glucocrossicanapin & 4-pentenyl ITC (**14**)  & x & \(\surd\) & x & \(\surd\) & x \\  & pentyl ITC (**15**)  & \(\surd\) & x & \(\surd\) & \(\surd\) & \(\surd\) & x \\  & phenylacetonitrile (**78**)  & \(\surd\) & \(\surd\) & x & \(\surd\) & x \\  & benzyl ITC (**21**)  & \(\surd\) & x & \(\surd\) & x & \(\surd\) \\  & 5-(methylsulfonyl)pentanonitrile (**79**)  & \(\surd\) & x & \(\surd\) & x & \(\surd\) \\  & (**79**)  & \(\surd\) & x & \(\surd\) & x & \(\surd\) \\  & 4+(methylthio)butanenitrile (**80**)  & \(\surd\) & x & \(\surd\) & x & \(\surd\) \\  & & & & & & \\  & & & & & & \\  & & & & & & \\  & & & & & & \\ \end{tabular}
\end{table} 
Table 5: Identified volatile glucosinolate hydrolysis products in the headspace of ‘salad’ rocket, wasabi, and watercress, and the presence/absence of their respective glucosinolate precursor. \(\beta\)-ionone (**98**).