## 3 Results and Discussion  
### In Vitro Culture Analysis  
The conducted study revealed a significant impact of the applied medium variants on the regeneration of nodal explants of Baikal skullcap (Table 3, Figure 2). The highest mean number of shoots (5.8) of the obtained regenerants was acquired on the MS + BAP \begin{table}
\begin{tabular}{l l l} \hline \hline
**No.** & **Starter Numer** & **Sequence (5’-3’)** \\ \hline
1 & SCoT-4 & CAACAATGGCTACCACC \\
2 & SCoT-12 & ACGACATGGCGACCAACG \\
3 & SCoT-14 & ACGACATGGCCACCACC \\
4 & SCoT-16 & ACCATGGTACCACCACC \\
5 & SCoT-24 & CACATGGCATCCACCACT \\
6 & SCoT-28 & CCTACGCTACCACCGCG \\
7 & SCoT-30 & CCTAGGCTACCCCGCCG \\
8 & SCoT-33 & CCTAGGCTACCACCGG \\
9 & SCoT-46 & ACAATGGCTACCACTGG \\
10 & SCoT-50 & ACAATGGCTACCACTGGG \\
11 & SCoT-71 & CCTAGGCTACCACCGGCG \\
12 & SCoT-75 & CCTAGGCTACCCCGAG \\ \hline \hline \end{tabular}
\end{table} 
Table 1: SCoT primer sequences. \begin{table}
\begin{tabular}{c c c} \hline \hline
**Step** & **Temperature** & **Time** \\ \hline Initial denaturation & 94 \({}^{\circ}\)C & 3 min \\  & No. of cycles = 35 cycles & \\ Denaturation & 94 \({}^{\circ}\)C & 1 min \\ Annealing & 50 \({}^{\circ}\)C & 1 min \\ Extension & 72 \({}^{\circ}\)C & 2 min \\ Final extension & 72 \({}^{\circ}\)C & 5 min \\ \hline \hline \end{tabular}
\end{table} 
Table 2: PCR amplification protocols using SCoT primers. 1 medium. The lowest mean number of shoots was obtained on the MS + KIN 2 (2.1). For the observed feature, no statistically significant differences were found on media with different KIN concentrations. These differences were also insignificant in relation to the MS \"0\" medium. On the medium with BAP at 0.25 and 0.5 mg/dm\({}^{3}\), the mean number of shoots was 2.7 and was also statistically insignificant. Increasing the BAP concentration resulted in an increase in the trait value to 5.8 (MS + BAP 1) and 4.3 (MS + BAP 2), respectively, but no statistically significant differences between them were proven. Regenerants from the MS + BAP medium exhibited a well-developed callus (that formed at the base of the shoot) and the highest mean weight. The composition of the medium had a fundamental influence on the observed mode of regeneration. The MS medium with the addition of kinetin initiated the process of direct shoot organogenesis. On the other hand, the process of indirect shoot organogenesis was stimulated by the MS medium with the addition of all concentrations of BAP. Since the regeneration process through this pathway occurs in two stages--the first involving the formation of callus tissue and the second involving the regeneration of shoots by some of its cells--high values were obtained for the presence of calluses and the mass of regenerants in this medium. In particular, the values of the latter were the highest on the MS + BAP 2 medium (statistically significant compared to the other media), which was due to the simultaneous presence of callus tissue and shoots in the structure of the regenerants (Figure 2H). [PERSON] et al. [18] pointed out the high effectiveness of BAP in combinations with various concentrations of TDZ, IBA, and NAA in the regeneration of _Scutellaria anaxensis_, concluding that the best effect in indirect shoot organogenesis was achieved with the combination of BAP and TDZ. According to a study by [PERSON] et al. [14] on _S. orientalis_ subsp. _bicolor_, the callus could be regenerated in the presence of BAP alone or in combination with NAA. However, an increase in the concentration of BAP had an inhibitory effect on its mass. In a study by [PERSON] et al. [20] concerning _S. bialcialensis_, the combination of BAP and NAA promoted shoot organogenesis and allowed them to obtain 1.4 to 8.5 shoots per explant. In addition, this combination of phytohormones caused the simultaneous formation of callus tissue. Such a response of explants in the present study occurred as a result of the independent action of BAP at concentrations from 0.25 to 2 mg/dm\({}^{3}\) in the medium, and the number of shoots produced varied from 2.7 to 5.8.