## 2 Materials and Methods
### Liquid Chromatography Mass Spectrometry
#### 2.4.2 Instrumental Analysis
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Compound** & \begin{tabular}{c} **Precursor Ion** \\ **Mass** \\ \end{tabular} & \begin{tabular}{c} **Mode of** \\ **Ionisation** \\ \end{tabular} & \
\begin{tabular}{c} **Product on** \\ **Mass** \\ \end{tabular} & **Q1 Pre Bias [V]** &
\begin{tabular}{c} **Collision** \\ **Energy [V]** \\ \end{tabular} & **Q3 Pre Bias [V]** \\ \hline \multirow{3}{*}{Genistein} & \multirow{3}{*}{270.9 [M+H]\({}^{+}\)} & \multirow{3}{*}{positive} & 152.8 & \(-\)13 & \(-\)33 & \(-\)23 \\ \cline{3-6}  & & & 118.8 & \(-\)30 & \(-\)33 & \(-\)19 \\ \cline{3-6}  & & & 90.8 & \(-\)13 & \(-\)44 & \(-\)14 \\ \hline \multirow{3}{*}{Apigenin} & \multirow{3}{*}{270.9 [M+H]\({}^{+}\)} & \multirow{3}{*}{positive} & 152.9 & \(-\)30 & \(-\)32 & \(-\)24 \\ \cline{3-6}  & & & 118.9 & \(-\)14 & \(-\)34 & \(-\)18 \\ \cline{3-6}  & & & 90.8 & \(-\)14 & \(-\)42 & \(-\)14 \\ \hline \multirow{3}{*}{_trans_-Chalcone} & \multirow{3}{*}{208.5 [M+H]\({}^{+}\)} & \multirow{3}{*}{positive} & 157.9 & \(-\)13 & \(-\)10 & \(-\)25 \\ \cline{3-6}  & & & 94.7 & \(-\)14 & \(-\)21 & \(-\)30 \\ \cline{3-6}  & & & 143.9 & \(-\)11 & \(-\)10 & \(-\)25 \\ \hline \multirow{3}{*}{Flavone} & \multirow{3}{*}{222.9 [M\(-\)H]\({}^{-}\)} & \multirow{3}{*}{negative} & 76.8 & \(-\)11 & \(-\)38 & \(-\)29 \\ \cline{3-6}  & & & 120.9 & \(-\)11 & \(-\)29 & \(-\)18 \\ \cline{3-6}  & & & 64.8 & \(-\)11 & \(-\)46 & \(-\)24 \\ \hline \multirow{3}{*}{Kaempfer} & \multirow{3}{*}{284.9 [M\(-\)H]\({}^{-}\)} & \multirow{3}{*}{negative} & 132.9 & 13 & 33 & 24 \\ \cline{3-6}  & & & 150.9 & 13 & 25 & 28 \\ \cline{3-6}  & & & 174.9 & 13 & 25 & 11 \\ \hline \multirow{3}{*}{Epicatechin} & \multirow{3}{*}{288.9 [M\(-\)H]\({}^{-}\)} & \multirow{3}{*}{negative} & 245.0 & 13 & 13 & 16 \\ \cline{3-6}  & & & 253.0 & 13 & 10 & 25 \\ \cline{3-6}  & & & 203.0 & 13 & 17 & 14 \\ \hline \multirow{3}{*}{Phloridzin} & \multirow{3}{*}{435.0 [M\(-\)H]\({}^{-}\)} & \multirow{3}{*}{negative} & 273.0 & 11 & 16 & 18 \\ \cline{3-6}  & & & 227.0 & 11 & 22 & 15 \\ \cline{3-6}  & & & 389.0 & 11 & 10 & 26 \\ \hline \multirow{3}{*}{Fisetin} & \multirow{3}{*}{285.2 [M\(-\)H]\({}^{-}\)} & \multirow{3}{*}{negative} & 133.0 & 14 & 32 & 24 \\ \cline{3-6}  & & & 150.9 & 14 & 23 & 29 \\ \cline{3-6}  & & & 174.9 & 14 & 24 & 17 \\ \hline \multirow{3}{*}{Biochanin A} & \multirow{3}{*}{282.9 [M\(-\)H]\({}^{-}\)} & \multirow{3}{*}{negative} & 255.0 & 13 & 17 & 17 \\ \cline{3-6}  & & & 226.9 & 30 & 24 & 15 \\ \cline{3-6}  & & & 226.0 & 30 & 27 & 10 \\ \cline{3-6}  & & & 150.9 & 30 & 17 & 23 \\ \cline{3-6}  & & & 119.0 & 12 & 22 & 20 \\ \hline \multirow{3}{*}{Luteolin} & \multirow{3}{*}{284.9 [M\(-\)H]\({}^{-}\)} & \multirow{3}{*}{negative} & 238.8 & 12 & 8 & 15 \\ \cline{3-6}  & & & 132.9 & 30 & 32 & 25 \\ \cline{3-6}  & & & 216.8 & 24 & 8 & 26 \\ \hline \multirow{3}{*}{Diosmetin} & \multirow{3}{*}{298.9 [M\(-\)H]\({}^{-}\)} & \multirow{3}{*}{negative} & 283.9 & 14 & 20 & 28 \\ \cline{3-6}  & & & 252.8 & 14 & 10 & 17 \\ \cline{3-6}  & & & 227.0 & 25 & 34 & 18 \\ \hline \multirow{3}{*}{Diosmin} & \multirow{3}{*}{607.0 [M\(-\)H]\({}^{-}\)} & \multirow{3}{*}{negative} & 299.2 & 30 & 30 & 20 \\ \cline{3-6}  & & & 283.7 & 30 & 49 & 18 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Flavonoid MRM details in studied rose damask cultivars.