## 3 Results and Discussion  
### Essential Oils Chemical Composition  
The complete composition and the hydrodistillation yield of the essential oil obtained from the dried cones of _H. Inplus_ Cascade are reported in Table 2. Altogether, 49 compounds were identified, accounting for up to 94.4% of the whole composition. \begin{table}
\begin{tabular}{l c c c} \hline \hline
**Compounds** & **l.r.i 1** & **Class.** & \multicolumn{2}{c}{**Relative Abundance**} \\  & & & **(\%) \(\pm\) SD** \\ \hline \(\beta\)-pinene & 977 & mh & 0.3 \(\pm\) 0.12 \\ myrcene & 991 & mh & 15.6 \(\pm\) 6.66 \\ linalool & 1101 & om & 0.1 \(\pm\) 0.11 \\ nonanal & 1105 & nt & 0.1 \(\pm\) 0.12 \\
2-undecanone & 1294 & nt & 0.3 \(\pm\) 0.01 \\ methyl 4-decenoate & 1312 & nt & 0.6 \(\pm\) 0.05 \\ methyl geranate & 1324 & om & 0.5 \(\pm\) 0.10 \\ \(\alpha\)-copaene & 1376 & sh & 0.3 \(\pm\) 0.00 \\ geranyl acetate & 1385 & om & 0.4 \(\pm\) 0.04 \\ \(\beta\)-caryophyllene & 1419 & sh & 9.0 \(\pm\) 0.03 \\ \(\beta\)-copaene & 1429 & sh & 0.1 \(\pm\) 0.10 \\ \(\mu\)ras-\(\alpha\)-bergamotene & 1436 & sh & 0.3 \(\pm\) 0.02 \\ \(\alpha\)-humulene & 1453 & sh & 28.9 \(\pm\) 0.04 \\ \((E)\)-\(\beta\)-farmesene & 1458 & sh & 3.8 \(\pm\) 0.12 \\ \(trans\)-cadina-1(6),4-diene & 1474 & sh & 0.2 \(\pm\) 0.06 \\ \(\beta\)-chamigrene & 1485 & sh & 0.5 \(\pm\) 0.17 \\ \(\gamma\)-muurolene & 1477 & sh & 1.0 \(\pm\) 0.08 \\ \(\alpha\)-amorphene & 1482 & sh & 0.2 \(\pm\) 0.00 \\ \(\beta\)-seline & 1486 & sh & 2.2 \(\pm\) 0.26 \\ \(\alpha\)-seline & 1495 & sh & 2.3 \(\pm\) 0.27 \\ \(2\)-ridecanone & 1496 & nt & 0.8 \(\pm\) 0.19 \\ \(epizonarene\) & 1500 & sh & 0.2 \(\pm\) 0.02 \\ \(\alpha\)-muurolene & 1501 & sh & 0.4 \(\pm\) 0.07 \\ _(E,E)_-\(\alpha\)-farmesene & 1509 & sh & 0.4 \(\pm\) 0.06 \\ _trans_-\(\gamma\)-cadinene & 1514 & sh & 1.8 \(\pm\) 0.24 \\ \(\delta\)-cadinene & 1524 & sh & 2.8 \(\pm\) 0.26 \\ cubeneene & 1533 & sh & 0.2 \(\pm\) 0.02 \\ \(\alpha\)-cadinene & 1537 & sh & 0.2 \(\pm\) 0.04 \\ \(\alpha\)-calacorene & 1543 & sh & 0.2 \(\pm\) 0.04 \\ elemol & 1550 & os & 0.2 \(\pm\) 0.07 \\ caryophyllene alcohol & 1563 & os & 0.3 \(\pm\) 0.01 \\ caryophyllene oxide & 1582 & os & 1.7 \(\pm\) 0.33 \\ _isoaromadendrene_ epoxide & 1586 & os & 1.5 \(\pm\) 0.31 \\ _epi_-globul & 1590 & os & 0.3 \(\pm\) 0.33 \\ humulene oxide II & 1608 & os & 5.2 \(\pm\) 0.99 \\ humulane-1-6-dien-3-ol & 1613 & os & 0.3 \(\pm\) 0.12 \\ l,10-di-_epi_-cubenol & 1615 & os & 0.2 \(\pm\) 0.05 \\ \(1\)-_epi_-cubenol & 1627 & os & 1.2 \(\pm\) 0.26 \\ caryophylla-4(14),8(15)-dien-5-ol & 1633 & os & 0.7 \(\pm\) 0.15 \\ T-cadinol & 1641 & os & 1.4 \(\pm\) 0.22 \\ T-muurolol & 1646 & os & 0.2 \(\pm\) 0.05 \\ \(\alpha\)-cadinol & 1654 & os & 1.5 \(\pm\) 0.18 \\ \(14\)-hydroxy-9-_epi_-(E)-caryophyllene & 1665 & os & 1.0 \(\pm\) 0.24 \\ aromandendrene epoxide II & 1680 & os & 0.6 \(\pm\) 0.08 \\ \(2\)-pentadecanone & 1699 & nt & 0.2 \(\pm\) 0.04 \\ _(E,E)_-farmesol & 1723 & os & 0.5 \(\pm\) 0.10 \\ _m_-camphorene & 1952 & dh & 0.3 \(\pm\) 0.03 \\ hexadecanoic acid & 1963 & nt & 3.1 \(\pm\) 0.36 \\ phytol & 2112 & od & 0.3 \(\pm\) 0.02 \\ \hline Total identified (\%) & & & 94.4 \(\pm\) 1.03 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Chemical composition and extraction yield of the essential oil from the cones of _Humulus_ l. Cascade. Sesquierpenes were the most represented chemical class of compounds in both their hydrocarbons and oxygenated derivatives, which reached 55.2% and 16.7%, respectively. \(\alpha\)-humulene (28.9%), \(\beta\)-caryophyllene (9.0%) and (E)-\(\beta\)-farnesene (3.8%) were the main chemicals belonging to the hydrocarbon form, and humulene oxide II (5.2%) to the oxygenated one. Monoterpenes were also quantitatively well represented, but almost only in their hydrocarbon form (16.0%). Among this class, only two volatile compounds were identified, but myrcene was the predominant, as it accounted for up to 15.6%. [PERSON] et al. (2016) and [PERSON] et al.