## 2 Results and Discussion
### General Features of the Genome  
Genome analysis was performed to characterize the genetic potential of this bacterium. To the best of our knowledge, this is the first study performing a genome analysis of _P. qingdaonensis_ species isolated from the rhizosphere of maize. The genome of _P. qingdaonensis_ ZCR6 was assembled into 52 contigs consisting of 5,507,067 bp with an average G + C content of 64.5%. Previously, similar genome sizes of different _Pseudomonas_ species isolated from the rhizosphere of various plants were reported [20; 22; 23]. A total of 5055 genes were annotated in the genome, of which 4943 were protein-coding sequences (CDSs). Furthermore, 8 rRNA-, 64 tRNA-, and 4 ncRNA-encoding genes were predicted. A large number of rRNAs is a typical characteristic of microorganisms, allowing them to respond rapidly to changing nutrient availability [24]. In addition, two intact prophage regions of 21.7 kb and 5.9 kb with GC contents of 62.29% and 60.54%, respectively, were identified. It is worth emphasizing that the presence of a prophage in the bacterial genome may increase its resistance to environmental stresses and biofilm formation and support horizontal gene transfer [25]. The general genome features of the ZCR6 strain are described in Table 3. The circular genome representing the entire genome assembly is shown in Figure 2. \begin{table}
\begin{tabular}{c c c} \hline \hline  & \multicolumn{2}{c}{**Percentage (\%)**} \\ \cline{2-3}  & **24 h** & **72 h** \\ \hline C16:0 & 30.95 & 22.52 \\ C16:1\(\omega\)7c and/or C16:1\(\omega\)6c & 26.35 & 11.42 \\ C18:1\(\omega\)7c and/or C18:1\(\omega\)6c & 15.44 & 11.68 \\ C17:0 cyclo & 10.22 & 23.07 \\ C12:0 2 OH & 4.63 & 8.74 \\ C12:0 3 OH & 4.11 & 7.57 \\ C10:0 3 OH & 3.34 & 4.35 \\ C12:0 & 2.22 & 4.07 \\ C 19:0 cyclo \(\omega\)8c & \textless{}1 & 5.14 \\ Others & \textless{}2 & \textless{}2 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Fatty acid composition (%) of the ZCR6 strain. \begin{table}
\begin{tabular}{l c} \hline \hline
**Feature** & **Value** \\ \hline Assembly information & \\ Genome size (bp) & 5,507,067 \\ Contigs & 52 \\ Conting N50 (bp) & 292,270 \\ Conting L50 & 7 \\ G + C content (\%) & 64.5 \\ Annotation information & 5055 \\ Genes (total) & 4979 \\ CDS (coding) & 4943 \\ CDSs (with protein) & 4943 \\ Pseudogenes & 36 \\ RNA genes & 76 \\ rRNA & 3, 3, 2 (55, 165, 23S) \\ tRNA & 64 \\ ncRNA & 4 \\ Prophage regions & 2 \\ Genes assigned to KEGG pathways & 2945 \\ Genes assigned to KEGG Orthology (KO) & 2209 \\ Genes assigned to COGs & 4877 \\ Genes connected to RASTtk & 2319 \\ BioProject ID & PRJNA681194 \\ BioSample ID & SAMN16935590 \\ GeneBank accession number & JADWSX000000000 \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Genome features of _P. qingdaonensis_ ZCR6. Figure 2: Circular plot representing the genome annotations of the ZCR6 strain. Colors depict the different classification types of gene clusters along the sequenced genome. The description of each circle is represented from the outermost circle to the innermost circle. Tick marks represent the predicted CDS on the positive and negative strands. AMR—antimicrobial resistance genes; VF—virulence factor genes. The COG database was used to functionally categorize predicted proteins. A total of 4877 protein-encoding genes in the ZCR6 strain were assigned to COG in 21 functional categories (Figure 3). This analysis revealed the highest gene abundance for amino acid transport and metabolism (E, 9.47%), transcription (K, 9.37%), inorganic ion transport (P, 8.35%), signal transduction mechanisms (T, 6.62%), energy production and conversion (C, 6.48%), and cell wall/membrane/envelope biogenesis (M, 6.15%). It is worth noting that the high percentage of genes belonging to the E and P classes may suggest the innate ability of the ZCR6 strain to compete with other microorganisms. It is also worth noting that the high metabolic activity of the studied strain may be reflected in the occurrence of many genes involved in the transcription process [25; 26].