## 3 Discussion
This may further be related to the effect of microdamage on increasing the cell's membrane potential and its secretory activity of proteins, macronutrients, and growth factors into the intercellular space, among others. These compounds, by stimulating the synthesis of fibroblasts in injured areas, initiate the synthesis of proteoglycans, elastin, and collagen. After micro-needle mesotherapy treatments, the remodeling phase and associated skin regeneration, in which numerous proliferation processes can be observed, occurs even within a few months [31]. The dynamic regeneration of the skin in the patients studied is also evidenced by a significant increase in the immunorepression of Ki-67 and PCNA in the dermis. The mentioned significance applies to patients in whom snail mucus was introduced using micro-needle mesotherapy (group A) and needle-free mesotherapy (group C). A statistically significant increase in MMP-2 immunodalization in the dermis in the aforementioned groups of patients, in whom the snail mucus preparation was used, testifies to its effect on the reorganization of ECM components. Furthermore, recent studies have explored the therapeutic potential of _Helix aspersa_ snail mucus. The extract exhibits antimelanogenic and antimuromal effects against melanoma cells, inhibiting melanin production, cell viability, migration, and invasion through reduced MMP2 expression [46]. It also shows cytotoxic activity against cutaneous T cell lymphoma lines, though without affecting MMP-9 expression [47]. In burn treatment, _H. aspersa_ extract cream accelerates healing and reduces pain compared to standard treatments [37]. The mucus demonstrates antimicrobial properties, particularly against Pseudomonas aeruginosa and weakly against _Staphylococcus aureus_, with the active compounds likely being proteins between 30 and 100 kDa [48]. These findings suggest that _H. aspersa_ mucus contains bioactive compounds with potential applications in cancer treatment, wound healing, and antimicrobial therapy, warranting further investigation into its therapeutic uses. Additionally, our results based on the comparison of morphometric and immunohistochemical analysis between groups indicate that 3 months after the treatment, micro-needle mesotherapy with snail filtrate improves skin condition. Of note, snail mucus has gained attention for its potential biomedical applications due to its unique properties and composition. It contains biologically active compounds such as glycolic acid, natural antibiotics, and glycoproteins [49]. The mucus can self-assemble into various structures, from nanoparticles to fibers, enhancing adhesion on smooth surfaces [50]. Researchers have successfully created biomimetic nanofibers through electrospinning, with fiber diameter controlled by solution concentration [49]. These nanofibers show promise in tissue engineering and wound healing applications. A study combining snail mucus with silver nanoparticles in PVA nanofibers demonstrated enhanced wound healing and antibacterial properties [51]. Snail mucus extract has also been found to affect human fibroblast viability, potentially influencing collagen and elastin production [52]. While the exact mechanisms of its beneficial effects require further research, snail mucus shows potential for various medical applications. Nowadays, women are more and more willing to use treatments to improve the condition of their skin. This is associated with developments in cosmetology and aesthetic dermatology. Procedures that are characterized by low invasiveness and lack of convalescence have gained popularity, as they do not exclude women from professional activity for a long time. In addition, we can observe an increase in interest in natural cosmetic raw materials dedicated to the care of mature skin with photoaging features, which include snail mucus. Conducting further research in the field of dermatology and aesthetic cosmetology will allow us to better understand the mechanisms leading to the regeneration process of skin with features of photoaging.