# CalculatetheROCcurve fpr,tpr,thresholds=roc_curve(expected,observed) roc_auc=auc(fpr,tpr)
The necessity for ethical review and approval was waived for this study due to the fact that patients had consented to the use of their data and had provided verbal consent to this use. The direct interaction with patients is not the responsibility of our department. Informed Consent Statement: Informed verbal consent has been obtained from patients for publication of this article. The direct interaction with patients is not the responsibility of our department. **Data Availability Statement:** No public involvement occurred in any aspect of this research, We comply with the code of ethics required and requested at the time. **Acknowledgments:** We are infinitely grateful to the patients, patients' relatives, administrators, researchers, doctors, nurses, technicians, and all the direct and indirect actors who carried out this wonderful study; [PERSON] bless them all, and for those who are no longer with us, we will always remember them with much love. We extend our gratitude to [PERSON] for his invaluable contributions to the creation of the dataset, as well as to our former PhD student in technology development, [PERSON]. Life is a paradigm in which the individual may exist in one moment and not in another. It hurts my soul to know that each patient and his or her family suffer, and I ask [PERSON] for comfort and refuge in His arms. We are grateful for the multidisciplinary linkage that generated the collaboration between different areas. **Conflicts of Interest:** The authors declare no conflicts of interest. ## Abbreviations

The following abbreviations are used in this manuscript:

ALL & Acute Lymphoblastic Leukemia

MTX & Methotrexate

HD-MTX & High-Dose Methotrexate

C677T & Polymorphism

A129C & Polymorphism

MTHFR & Methylenetetrahydrofolate Reductase

MTXPG & Methotrexate Polyg glutamate

SLCO1B1, SLC19A1,

SLC22A8 and MTHFD1 & of acute toxicity after

HD-MTX treatment

MTHFR C677T & Common changes in MTHFR

MTHFR, ABCB1, ABCC2,

and TYMS & Warms from to be associated with toxicity;

they also found a statistically significant association

between MTHFR rs1801133 and anemia

in the consolidation phase

MTHFR rs1801133 & Frequency of the c677t polymorphism

SLCO1B1 rs4149056 & Polymorphism

C_24h,AUC_48h & MTX pharmacokinetic parameters

RFC1 & Reduce Folate Carrier

MTRR & Methionine Synthase Reductase

DFR & Enzyme Di-Hydrofolate Reductase

ANN & Artificial Neural Network

GUIDE & Graphical User Interface

WHO & World Health Organization (WHO)

GUI & Graphical User Interface

## References

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* [PERSON] et al. (2020) [PERSON]; [PERSON]; [PERSON]; [PERSON] Association of microRNA Polymorphisms with Toxicities Induced by Methotrexate in Children with Acute Lymphoblastic Leukemia. _Hemalot. Rep._**2023**, _15_, 634-650. [CrossRef] [PubMed]
* [PERSON] (2016) [PERSON] Childhood leukaemia: An update. _Paeiditar.