## 1 Introduction  
Polysaccharides, a class of natural macromolecule polymers which consist of more than 10 monosaccharides linked with glycosidic bonds, have a wide range of biological activities [1]. Edible mushroom polysaccharides (EMPs) are a member of the polysaccharide family. As natural compounds, EMPs are very good candidates for developing functional foods and nutraceuticals due to their various biological activities and relatively low toxicity [2]. EMPs have shown a wide range of biological functions, such as anti-tumor, anti-bacterial, antioxidant, antiviral, regulating gut microbiota and immunomodulatory activities [2; 3; 4]. Among them, immunomodulatory activity of polysaccharides is one of the hotspots in current research. A variety of EMPs are involved in the regulation of innate immunity and acquired immunity by influencing various important immune organs [2; 5], immune cells and immune factors [3]. Some edible mushrooms are also involved in Chinese herbal medicine such as _Ganoderma lucidum_ and _Polyporus umbellatus_. The mechanisms of Chinese herbal medicine polysaccharides are considered to participate in the activity regulation of T cells, B cells, macrophages, dendritic cells and natural killer (NK) cells, binding to specific receptors on the cell surface, activating various signal transduction pathways in the cell, stimulating cytokine or antibody secretion, and thus regulating the immune function of the body at multiple levels, multiple channels and multiple targets [6]. In recent years, there have been many studies focusing on the immunomodulation of polysaccharides from the same source, but few studies have focused on the functions of the immune system of polysaccharide composition from different sources. However, considering the complex structure of polysaccharides, the immunomododulation of polysaccharides from different sources may be different. Therefore, whether the composition of polysaccharides from different sources can coordinate immunomododulation has been of interest. Both _G. lucidum_ and _P. umbellatus_ are valuable edible and medicinal mushrooms with a history of use for more than 2000 years in China in the treatment diseases and regulation the body balance [7; 8; 9]. Ancient Chinese medical scholars suggested that _G. lucidum_ had the activity of \"Fuzheng Guben\", which means it could strengthen body resistance and consolidate the constitution of patients [10], while _P. umbellatus_ could induce diuresis and excrete dampness [11]. According to traditional Chinese medicine theory, the combination of _G. lucidum_ and _P. umbellatus_ was meaningful because the combination had the characteristics of supplement without causing stagnation. Current research has found that _G. lucidum_ and _P. umbellatus_ polysaccharides mainly exhibited immunoregulation, which adjusted the function of a variety of immune cells including T cells, B cells, macrophages, dendritic cells and NK cells [8; 12; 13]. _G. lucidum_ polysaccharides (GLP) and _P. umbellatus_ polysaccharides (PUP) in combination with chemotherapy drugs enhance efficacy and reduce toxic side effects via the immune system, respectively [14; 15]. Above all, it was hypothesized that the combination of GLP and PUP may regulate the immune function more effectively. Macrophages are immune cells derived from progenitor cells in the bone marrow [16] which play an important bridging role in innate immunity and acquired immunity [17]. As a key member of the innate immune defense front of the body, macrophages exist in almost all tissues, and their main function is to engulf and degrade dead cells, cell debris and foreign pathogens, and coordinate the inflammatory process [18; 19]. In addition, macrophages are capable of presenting antigens, secreting a variety of active substances, regulating the local microenvironment and other physiological functions [20]. Currently, it has been reported that GLP and PUP obtained by different extraction methods affected the activity of macrophages.