## 3 Results
### Alleplopathic Effects of juglone in the Field Enclosure Bioassays
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Classifications** & **Algal Species** & **Day 0** & **Day 7** & **Day 7** & **Day 7** \\  & & & **(Control)** & **(0.1 mg L-1)** & **(1 mg L-1)** \\ \hline \multirow{7}{*}{Cyanobacteria} & _Anabaena fios-aquae_ & 15,000 \(\pm\) 3000 & 29,500 \(\pm\) 24,500 & 3750 \(\pm\) 2250 & 20 \(\pm\) 20 \\  & _Menisropolini glucum_ & nd & 480 \(\pm\) 160 & 608 \(\pm\) 352 & nd \\  & _Microopsis aeruginosa_ & 21,400 \(\pm\) 3000 & 27,500 \(\pm\) 1300 & 24,600 \(\pm\) 3800 & 23,400 \(\pm\) 2600 \\  & _Oscillatoria curriccipes_ & 52,000 \(\pm\) 4000 & 17,000 \(\pm\) 7000 & 13,400 \(\pm\) 1400 & 600 \(\pm\) 600 \\  & _Phormidium subfuscum_ & 74,000 \(\pm\) 20,000 & 88,000 \(\pm\) 10,000 & 13,550 \(\pm\) 4450 & 3400 \(\pm\) 3400 \\ \hline \multirow{7}{*}{Green algae} & _Anbistroschus glaucatus_ & 80 \(\pm\) 20 & 20 \(\pm\) 20 & 75 \(\pm\) 45 & 30 \(\pm\) 30 \\  & _Ccalibrant spp._ & nd & 48 \(\pm\) 16 & nd & nd \\  & _Peliasstrum duplex_ & 240 \(\pm\) 80 & 120 \(\pm\) 40 & 116 \(\pm\) 84 & 48 \(\pm\) 16 \\  & _Peliasstrum simplex_ & 240 \(\pm\) 0 & 160 \(\pm\) 80 & 320 \(\pm\) 80 & 76 \(\pm\) 44 \\  & _Scenedes accuminants actimatus_ & 280 \(\pm\) 200 & 100 \(\pm\) 20 & 770 \(\pm\) 130 & 30 \(\pm\) 30 \\  & _Scenedesmus ecornis_ & 1040 \(\pm\) 160 & 3320 \(\pm\) 1480 & 6900 \(\pm\) 1700 & 4200 \(\pm\) 580 \\  & _Scenedesmus quadriciunda_ & 280 \(\pm\) 40 & 500 \(\pm\) 100 & 1760 \(\pm\) 1.440 & 1300 \(\pm\) 300 \\  & _Schroroeleria nitzschioitats_ & 500 \(\pm\) 100 & 30 \(\pm\) 30 & 410 \(\pm\) 110 & 10 \(\pm\) 10 \\ \hline \multirow{7}{*}{Others} & _Anbicoseira spp._ & 340 \(\pm\) 20 & nd & nd & nd \\  & _Cyclotella spp._ & 220 \(\pm\) 100 & 340 \(\pm\) 60 & 100 \(\pm\) 20 & 390 \(\pm\) 170 \\ \cline{1-1}  & _Certain himitatellia_ & 4 \(\pm\) 1 & nd & nd & nd \\ \cline{1-1}  & _Peliatinum bipes_ & 175 \(\pm\) 25 & 80 \(\pm\) 70 & 25 \(\pm\) 5 & 35 \(\pm\) 25 \\ \cline{1-1}  & _Cryptomonas ovata_ & 200 \(\pm\) 40 & 110 \(\pm\) 70 & 10 \(\pm\) 10 & nd \\ \cline{1-1}  & _Trachelomonas sp._ & 15 \(\pm\) 5 & nd & nd & nd \\ \hline Total number of species & 19 & 17 & 16 & 15 & 13 \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Changes in algal species and abundance (cells mL-1) after treatment with different concentrations of jugloene in the enclosure bioassay. Data are presented as averages with standard errors of duplicate experiments. Figure 4: Growth responses (% cell density relative to control) of dominant algal species in the field enclosures after the addition of jugloene (0.1 and 1 mg L-1). Data are presented as averages with standard errors (bars) of duplicate experiments. Three of these dominant cyanobacteria, _O. curriceps_, _P. subfuscum_, and _A. flos-aquae_, were inhibited by 21.2 \(\pm\) 8.0%, 84.6 \(\pm\) 5.0%, and 87.3 \(\pm\) 6.0%, respectively, after treatment with 0.1 mg L\({}^{-1}\) of judging, and by 96.5 \(\pm\) 1.5%, 96.1 \(\pm\) 1.8%, and 98.3 \(\pm\) 0.0%, respectively, at 1 mg L\({}^{-1}\) judging (Figure 4). The cell density of _M. aeruginosa_ decreased by 10.5 \(\pm\) 14.0% and 15.0 \(\pm\) 9.0% after treatment with 0.1 and 1 mg L\({}^{-1}\) juglone, respectively (Figure 4), and these reduction rates were much lower than those observed in the laboratory results (37.7 \(\pm\) 6.9% and 92.7 \(\pm\) 5.4%, respectively). The growth of two green algae, _S. quadricauda_ and _S. ecornis_, was stimulated (Figure 4), as observed in the laboratory bioassays. Notably, the cell density of _S. quadricauda_ remarkably increased by 350 \(\pm\) 59.0% and 250 \(\pm\) 65.0% compared to that of the control after treatment with 0.1 and 1 mg L\({}^{-1}\) juglone, respectively. The cell density of _S. ecornis_ also increased by 208 \(\pm\) 26.0% and 125 \(\pm\) 18.0% after treatment with 0.1 and 1 mg L\({}^{-1}\) juglone, respectively.