## 3 Results and Discussion
### Study of the Physicochemical Properties of Aqueous IgG Colloid of Different Concentrations
It is known that this relationship characterizes the change in adiabatic elasticity and/or density of the medium [60]. From Figure 5b, it can be assumed that the density associated with dynamic viscosity changes predominantly, while the adiabatic elasticity of the medium should not change significantly. The impact of an external magnetic field with frequencies of 8 and 50 Hz, as well as mechanical stirring with frequencies of 4.4 and 12 Hz, on the specific electrical conductivity of an aqueous colloid of IgG protein was investigated (Figure 6a). The specific electrical conductivity of H\({}_{2}\)O and the aqueous protein colloid changes under the action of mechanical mixing with frequencies of 4.4 and 12 Hz. Fundamentally, this is not a priority result, since an increase in the electrical conductivity of H\({}_{2}\)O and aqueous solutions after exposure to mechanical stirring was shown previously [61]. It should be noted that the conductivity of water under mechanical action increases by almost 50%, while under the action of mechanical shaking on protein colloids, the conductivity of H\({}_{2}\)O increases only by 10-20%. It was previously assumed that an increase in the specific electrical conductivity of H\({}_{2}\)O under mechanical action is associated with the formation of reactive oxygen species (ROS) [62; 63]. It is believed that the generation of reactive oxygen species begins with the activation of O\({}_{2}\) dissolved in H\({}_{2}\)O [64]. Briefly, the chain of chemical transformationsprobably looks like this: an oxygen molecule is activated, goes into the singlet state, and attaches an electron, which leads to the formation of a superoxide anion radical. Under normal conditions, superoxide anion radicals are immediately protonated, which leads to the hydroperoxide radical formation, the dismutation of which produces H\({}_{2}\)O\({}_{2}\) and O\({}_{2}\) (in the singlet state at 25%) [65; 66]. There is an alternative point of view, according to which the water molecule undergoes dissociation into a hydroxyl radical, a proton, and an electron [67]. In this regard, it is important to investigate the influence of the applied physical influences on the concentration of O\({}_{2}\) in the liquid phase of H\({}_{2}\)O and protein  colloids. The impact of an alternating magnetic field with frequencies of 8 and 50 Hz, as well as mechanical stirring with frequencies of 4.4 and 12 Hz, on the concentration of O\({}_{2}\) in the colloid of IgG protein molecules was investigated (Figure 6b). It has been shown that the concentration of O\({}_{2}\) in H\({}_{2}\)O and aqueous protein colloids changes under the influence of mechanical stirring with frequencies of 4.4 and 12 Hz; when exposed to an alternating magnetic field, a small statistically significant effect is observed at an IgG concentration of 0.1 mg/mL, although the trend is toward an increase in concentration when exposed to a magnetic field, and it is also noticeable for other IgG concentrations. In the case of mechanical action, a rather interesting effect is observed, namely, with mechanical action on  
Figure 6: Changes in specific electrical conductivity (**a**), molecular oxygen concentration (**b**), redox potential (**c**), and pH (**d**) of aqueous colloid of IgG protein molecules after exposure to alternating magnetic field with frequencies of 8 and 50 Hz, as well as mechanical mixing effect with frequencies of 4.4 and 12 Hz (M \(\pm\) SD, n = 6). **—**statistically significant differences relative to unexposed samples (_t_-test, \(p\) < 0.05). H\({}_{2}\)O, the concentration of O\({}_{2}\) decreases, and when exposed to a protein colloid, it increases. It was previously shown that when an aqueous solution of sodium chloride saturated with atmospheric gases is subjected to mechanical action, a decrease in the concentration of O\({}_{2}\) is observed [68]. It has been shown that with mechanical impact on the protein colloid, the oxygen concentration, on the contrary, increases.