## 4 Discussion  
### Chemical Analysis  
The results of the qualitative assessment of EOL were similar to those found in several published papers dealing with Laurel leaves EO, pointing out abundance in 1,8-cineole, followed by \(\alpha\)-terpinyl acetate, sabinene, and linalool [7; 11; 13; 15; 30]. Besides the investigations of EOs obtained using the classical hydrodistillation method, recently [PERSON] et al. obtained laurel leaves isolates by supercritical fluid extraction (SFE) at different pressures/temperatures of 100 bar/40 \({}^{\circ}\)C and 250 bar/40 \({}^{\circ}\)C, revealing that the main component was also 1,8-cineole. However, when a higher temperature was employed (the condition of SFE set at 100 bar/60 \({}^{\circ}\)C), \(\alpha-\)terpineol acetate was a dominant constituent [8]. EO from laurel fruit, EOF, exhibited a similar chemical composition pattern compared to EOL, with a slight difference in the relative percentage of 1,8-cineol, being more abundant in EOL (35.1% and 33.3% in EOL and EOF, respectively), but with \(\alpha\)- and \(\beta\)-pinenees and fatty acids detected in a higher percentage (Table 1, Figure 3). The obtained results were in line with the published data, with differences that might be ascribed to the different geographical origins of the investigated samples used in our study [31]. In addition, in EOF phenylpropanol (_E_)-2-hexyl-cinnamaldehyde was detected (1.7%), but methyl eugenol, present in EOL, was not detected in EOF (Table 1). Data from the literature revealed that the main compounds of the fruit EOs in previous studies were 1,8-cineole (8.10-48.0%), \(\alpha\)-terpinyl acetate (3.67-10.4%), sabinene (4.49-11.4%), \(\alpha\)-phellandrene, eugenol, methyl eugenol, \(\alpha\)-pinene, \(\beta\)-ocimene, and \(\beta\)-pinene (3.91-12.8%) [32]. The differences between the Montenegro laurel EOs composition in this study and those from other countries reported in the literature were probably due to the different plant genotypes and climatic and ecological conditions in the respective locality of the National  
Figure 3: The comparative presentation of the main constituents present in the investigated essential oils, EOL and EOF. Park where the plants were grown but also to the plant parts processed and extracted, including methods of harvesting, processing, drying, and optimization of technological processes for obtaining EOs [13]. [PERSON] et al. performed an investigation regarding the chemical composition and the possible anti-proliferative activity of leaves and fruits EOs [14]. Similarly, [PERSON] et al. dealt with both the chemical profile and antimicrobial activity of EOs from laurel leaves and fruits originating from Bulgaria, emphasizing the influence of various factors, e.g., growth environment, harvest season, plant parts used, and extraction method on the yield and chemical composition [15]. Compared to other already performed studies, the novelty of our research was in the investigation of fatty oil obtained from the fruit after EO was extracted by hydrodistillation as a waste material, representing a possible source of bioactive compounds. Obtained fatty oil contained high amounts of saturated fatty acids, namely lauric and palmitic acids, which was in accordance with the already published data. The contents of oleic acid and linoleic acid (Table 1) were lower in comparison to results presented in previous works [31; 33; 34; 35], while the most abundant component detected in our fatty oil was sesquiterpene lactone dehydrocostuslactone (21.7%). Phenylpropanol (_E_)-2-hexyl-cinnamaldehyde was detected in significant amounts in our sample (relative percentage of 5%), but the literature survey revealed that this compound was not mentioned in the previously conducted research. Hence in the analysed fatty oil, in addition to fatty acids, the presence of sesquiterpene lactones (dehydrocostuslactone, costunolide, and dehydrocostunolide) was of particular interest. Namely, [PERSON] et al.