## 3 Discussion  
### Thin-Layer Chromatography (TLC)  
Initial characterization of secondary metabolite groups allowed for the estimation of the relevance of a species producing compounds that could potentially become bioactive candidates and drive further investigations [5; 31].  
With chemical revelation using UV light and natural product reagent/poly ethylene glycol (NP/PEG) spray, the bands became even more evident at a wavelength of 254 nm due to the fact that all flavonoids cause fluorescence. The different colors at 365 nm (yellow, green, or blue) were attributed to the different structures of the compounds, as stated by [32; 33]. In the ethyl acetate partition, the four distinct yellow fluorescent bands suggest the presence of flavonoid glycosides. The retention factor (Rf) value of 0.26 also matches bands observed in the crude and methanolic extracts, indicating the possible presence of glycosylated quercetin and/or kaempfer. The Rf value of 0.35 suggests the presence of the flavonol glycoside rutin. Orientin and vitexin could be the glycosylated flavones indicated by the Rf value of 0.49 in the ethyl acetate partition sample. An Rf value of 0.61 was observed for a blue fluorescent band, which remains uninterpreted. In the crude extract, a band with an Rf value of 0.31 was detected, which may correspond to quercetin or kaempfer. All suggested identifications of compounds in this chromatographic analysis were based on Wagner and Bladt [33].  
ularly \(\beta\)-carbonyls present in extracts [35] from _Banisteriopsis caapi_, and also the possibility of purine alkaloids or pseudo-alkaloids. The absence of alkaloids was indicated by the lack of color change in the plates, consistent with other Malpighiaceae species that do not contain alkaloid compounds in their structures [36], such as _Hetetroperys tomentosa_, and nitrogenous compounds as seen in the phytochemical prospecting of hydroethanolic leaf extracts and fractions of various polarities from _Banisteriopsis laeifolia_[26]. The variability in the presence of alkaloids in Malpighiaceae is recurring; a study found that both young and older stems of _B. caapi_ exhibited a weak reaction for alkaloid detection, even though this class of phytochemicals is more common in _Banisteriopsis_, _Diplopterys_, _Tetraperys_, and _Stigmaphlyllon_[12].  
The species _D. pubipetatala_ is used analogously to _B. caapi_ in the preparation of Ayahuasca tea, particularly due to its production of alkaloids [37]. It is involved in traditional indigenous rituals in the Western Amazon region, providing experienced individuals with sensations recognized as mystical and spiritual experiences due to the presence of \(\beta\)-carbonyl alkaloids: harmine, harmaline, tetrahydrocharmine, and harmol [35], along with increased serotonin concentrations and availability of N,N-dimethyltryptamine (DMT) facilitated by mixing with another species [12]. Investigations treat the components of this tea as responsible for aiding in recovery from [PERSON]'s disease and Alzheimer's disease [38].  
Psychotherapeutic results of this tea support improvements in behavioral health, emotional regulation, and cognitive flexibility [39].