## 2 Results and Discussion
### Volatile Maillard Reaction Products Formation  
In this study, GC-MS with SPME technique was used to identify the volatile compounds of rye-buckwheat biscuits. The results for identified volatiles are shown in Tables 2 and 3. The main identified volatile compounds belong to aldehydes, terpen and pyrazines. Figure 1: Aroma profiles of control (CB), rye-buckwheat biscuit (RBB) and RBBs with low, medium and high rutin addition (RBB-L, RBB-M, RBB-H, respectively). \begin{table}
\begin{tabular}{l c c c c c c c c} \hline \hline
**Compound** & **R1\({}^{\text{a}}\)** **Ltr** & **R1\({}^{\text{b}}\)** & **Odor Description** & **CB** & **RBB** & **RBB-L** & **RBB-M** & **RBB-H** \\ \hline Aldehydes and ketones & & & & & & & & & \\
2-methylpropanol & 611 & 625 & maly & 13.91 \(\pm\) 0.91 \({}^{\text{c}}\) & 24.90 \(\pm\) 0.14 \({}^{\text{a}}\) & 19.81 \(\pm\) 0.12 \({}^{\text{b}}\) & 9.48 \(\pm\) 0.53 \({}^{\text{e}}\) & 10.76 \(\pm\) 0.47 \({}^{\text{d}}\) \\
2-methylbutanal & 912 & 927 & maly & 347.36 \(\pm\) 19.31 \({}^{\text{d}}\) & 591.31 \(\pm\) 12.60 \({}^{\text{c}}\) & 631.33 \(\pm\) 32.52 \({}^{\text{c}}\) & 967.80 \(\pm\) 55.38 \({}^{\text{b}}\) & 1087.94 \(\pm\) 37.53 \({}^{\text{a}}\) \\ Pentanal & 935 & 946 & pungent & 15.11 \(\pm\) 0.39 \({}^{\text{b}}\) & 13.85 \(\pm\) 0.52 \({}^{\text{c}}\) & 14.75 \(\pm\) 0.51b \({}^{\text{c}}\) & 36.47 \(\pm\) 0.04 \({}^{\text{a}}\) & 35.58 \(\pm\) 0.05 \({}^{\text{a}}\) \\ (E)-cinnamaldehyde & 1631 & 1641 & cinnamon & 354.82 \(\pm\) 13.00 \({}^{\text{a}}\) & 354.19 \({}^{\text{a}}\) & 23.66 \({}^{\text{a}}\) & 39.69 \(\pm\) 4.67 \({}^{\text{a}}\) & 355.24 \(\pm\) 44.00 \({}^{\text{a}}\) & 302.82 \(\pm\) 51.19 \({}^{\text{a}}\) \\ 2-heptanone & 1160 & 1175 & soapy & 11.62 \(\pm\) 0.22 \({}^{\text{b}}\) & 80.11 \(\pm\) 0.19 \({}^{\text{a}}\) & 6.90 \(\pm\) 0.90 \({}^{\text{a}}\) & 12.73 \(\pm\) 0.84 \({}^{\text{a}}\) & 14.05 \(\pm\) 1.71 \({}^{\text{a}}\) \\ 2-nonanone & 1280 & 1289 & soap, green & 7.69 \(\pm\) 0.71 \({}^{\text{a}}\) & 6.39 \(\pm\) 0.44 \({}^{\text{a}}\) & 7.58 \(\pm\) 0.16 \({}^{\text{b}}\) & 8.23 \(\pm\) 0.37 \({}^{\text{a}}\) & 8.56 \(\pm\) 0.16 \({}^{\text{a}}\) \\ Tepenes & & & & & & & & \\ \(\alpha\)-pinene & 1034 & 1039 & rosin & 0.45 \(\pm\) 0.02 \({}^{\text{a}}\) & 0.40 \(\pm\) 0.07 \({}^{\text{a}}\) & 0.41 \(\pm\) 0.06 \({}^{\text{a}}\) & 0.38 \(\pm\) 0.01 \({}^{\text{a}}\) & 0.41 \(\pm\) 0.02 \({}^{\text{a}}\) \\ \(\beta\)-myrcrene & 1176 & 1156 & gparanium, sweet & 0.18 \(\pm\) 0.02 \({}^{\text{a}}\) & 0.12 \(\pm\) 0.04 \({}^{\text{a}}\) & 0.14 \(\pm\) 0.02 \({}^{\text{a}}\) & 0.17 \(\pm\) 0.07 \({}^{\text{a}}\) & 0.22 \(\pm\) 0.02 \({}^{\text{a}}\) \\ 1,8-cinole & 1224 & 1234 & eucalyptus-like & 26.50 \(\pm\) 1.48 \({}^{\text{a}}\) & 27.38 \(\pm\) 2.05 \({}^{\text{a}}\) & 20.67 \(\pm\) 4.85 \({}^{\text{a}}\) & 21.59 \(\pm\) 5.15 \({}^{\text{a}}\) & 27.98 \(\pm\) 0.21 \({}^{\text{a}}\) \\ D-Innomee & & & & & & & & & \\ Caryophyllene & 1234 & 1244 & eucalyptus-like & 22.02 \(\pm\) 13.6 \({}^{\text{a}}\) & 231.22 \(\pm\) 22 \({}^{\text{a}}\) & 29.61 \(\pm\) 51.5 \({}^{\text{a}}\) & 26.21 \(\pm\) 1.61 \({}^{\text{a}}\) & 23.15 \(\pm\) 0.41 \({}^{\text{a}}\) \\ Caryophyllene & 1594 & 1604 & wood, spicy & 83.04 \(\pm\) 6.05 \({}^{\text{c}}\) & 123.31 \(\pm\) 14.10 \({}^{\text{a}}\) & 124.66 \(\pm\) 18.64 \({}^{\text{a}}\) & 104.40 \(\pm\) 9.89 \({}^{\text{b}}\) & 136.29 \(\pm\) 10.79 \({}^{\text{a}}\) \\ Other & & & & & & & & \\ Estzagleo & 1655 & 1666 & licorice, anise & 51.06 \(\pm\) 5.11 \({}^{\text{a}}\) & 7.62 \(\pm\) 1.86 \({}^{\text{b}}\) & 7.31 \(\pm\) 0.28 \({}^{\text{b}}\) & 7.02 \(\pm\) 0.13 \({}^{\text{b}}\) & 7.87 \(\pm\) 0.37 \({}^{\text{b}}\) \\ \hline \hline \end{tabular}  
* Retention indexes from literature: spherosa.org for DB-WAX. \({}^{\text{b}}\) Calculated retention index values relatively to the n-alkane ladder. Values are means \(\pm\) standard deviation (n = 3). Values in each column with different small superscript letters are significantly different (\(p<0.05\)).  
\end{table}