## 1 Introduction  
Mango (_Mangifera indica_ L.) is a popular fruit with a strong and distinctive flavor that is widely grown in many tropical and subtropical countries [1]. However, during the growth stage, the mango fruit and leaves are often affected by anthracose disease, in which _Colletotrichum gloeosporoides_ fungi cause severe lesions with black spots. Some estimates have placed the incidence of anthracose disease in farmers' fields as high as 36%-74% [2]. Traditionally, _C. gloeosporoides_ fungi are treated by chemical spraying. However, the fungi have gradually developed resistance to commercial fungicides, and hence alternative methods for combating anthracose disease are required [3]. Metallic antimicrobial surfaces are widely used for the storage and delivery of water. For example, studies have shown that Ag bottles and vessels provide an effective means of preventing the spoilage of stored water by bacteria [4]. Furthermore, surface chemistry modifications of such surfaces--combined with proper cleaning products and procedures--produces a further improvement in antimicrobial performance [5]. In recent years, the antifungal activity of nanoparticles (NPs) against some phytopathogenic fungi has been investigated [6]. Many studies have demonstrated the effectiveness of Ag, Cu and ZnO nanoparticles (NPs) in inhibiting the activity of phytopathogenic fungi [3; 7; 8; 9]. Ag NPs show a particularly strong bactericidal performance: with silver additions, the textile has antimicrobial activities, while thin films coated on the substrate have an antifungal function. [PERSON] [10] reported that chitosan-AgNP composite exhibits a higher antifungal activity against _C. gloeosporoides_ than its components [11]. The biological activity of metallic glass materials hasalso attracted significant attention in recent years [12; 13]. [PERSON] [14] reported that the growth area of microbes on Zr-based metallic glass films was smaller than that on SUS304 substrates. Furthermore, the coatings exhibited a particularly long-term antimicrobial effect for _Pseudomonas aeruginosa_. The thin film metallic glasses extended the attainable size of the metallic glass and showed a potential application in the medical field. Nowadays, most applications on plant pathogens use nanomaterials as fungicides [15]. Studies have shown that Cu nanoparticles inhibit plant pathogens such as _Phoma destructura_, _Curvularis lunata_ and _Alternaria_, while Ag particles suppress the growth of _Macrophomina phaseolina_ and _Rhizcotonia solani_[7]. Therefore, nanoparticles have potential for development as a bacteriostatic or fungicide. Various techniques are available for the production of micro- and nanoscale metallic particles, including mechanochemical methods [16], green synthesis [17], spray pyrolysis [18], sol-gel [19], chemical vapor deposition [20] and dewetting [21]. In the latter method, a metallic film is deposited on a glass substrate then annealed, resulting in a dewetting phenomenon that minimizes the total energy of the free surfaces of the film and substrate. Compared to other methods, dewetting has the advantages of large area coverage, low cost and production of ordered NPs. Furthermore, the rapid thermal treatment of thin Ag/Cu bilayers also enables the production of composite Ag-Cu nanoparticles [22]. However, the high melting temperatures of most metals are not easily achievable using conventional heating methods. Therefore, the dewetting process is generally performed using high-energy sources such as focused ion beams and lasers [23]. The feasibility of pulsed laser-induced dewetting, especially using nanosecond pulses, has attracted significant attention in the literature [24; 25; 26; 27; 28; 29; 30]. However, only scant information is available regarding the synthesis of metallic NPs using a high repetition rate laser. Accordingly, the present study prepares Ag particles with various sizes using a high repetition rate pulsed laser and an Ag film with a carefully controlled thickness.