## 3 Results
### Validation  
As for inclusivity and exclusivity, the results showed all samples were correctly recognised; as a matter of fact, all target microorganisms were identified, while the relevant range of other species tested did not interfere.  
The relative accuracy, specificity, and sensitivity of each food category are reported in Table 2.  
\begin{table}
\begin{tabular}{c c c c} \hline \hline  & \multicolumn{3}{c}{**Microorganism**} \\ \hline
**Tested for Inclusivity** & \multicolumn{3}{c}{**Species Used for Exclusivity Testing**} & **Code** \\ \hline
1 & _[PERSON]_ & 1 & _Aerononas hydrophila_ & ATCC 35650 \\
2 & _[PERSON]_ & 2 & _Arocbacter butzleri_ & NCTC 12481 \\
3 & _[PERSON]_ & 3 & _Bacillus cereus_ & ATCC 11778 \\
4 & _[PERSON]. [PERSON]_ & 4 & _Bacillus cereus_ & B25052 \\
5 & _[PERSON]. Typhimuriumurin (monoplasmic)_ & 5 & _Bacillus subtilis_ & BCS51 \\
6 & _[PERSON]_ & 6 & _Campylobacter coli_ & ATCC 33559 \\
7 & _[PERSON]_ & 7 & _Campylobacter jejuni_ & ATCC 33291 \\
8 & _[PERSON]_ & 8 & _Citrobacter freudii_ & ATCC 8990 \\
9 & _[PERSON]_ & 9 & _Clostridium biferentans_ & CBIF107 \\
10 & _[PERSON]_ & 10 & _Clostridium perfingens_ & ATCC 13124 \\
11 & _[PERSON]_ & 11 & _Escherichia coli_ & ATCC 25922 \\
12 & _[PERSON]_ & 12 & _Escherichia coli_ & O157 \\
13 & _[PERSON]_ & 13 & _Enterobacter cloaeae_ & Not available \\
14 & _[PERSON]_ & 14 & _Enterococcus ficeium_ & EFC49 \\
15 & _[PERSON]_ & 15 & _Enterobacter sakazkii_ & ATCC 29544 \\
16 & _[PERSON]_ & 16 & _Listeria innocua_ & ATCC 33090 \\
17 & _[PERSON]. [PERSON]_ & 17 & _Listeria innocui_ & ATCC 19119 \\
18 & _[PERSON]_ & 18 & _Listeria monocytogenes_ & ATCC 7684 \\
19 & _[PERSON]_ & 19 & _Listeria selfiç_ & Not available \\
20 & _[PERSON]_ & 20 & _Microoccus luteus_ & ATCC 9341 \\
21 & _[PERSON]_ & 21 & _Psudomonas aeruginosa_ & ATCC 10145 \\
22 & _[PERSON]_ & 22 & _Rhodococcus equi_ & ATCC 6939 \\
23 & _[PERSON]_ & 23 & _Staphylococcus aureus_ & ATCC 25923 \\
24 & _[PERSON]_ & 24 & _Staphylococcus aureus_ & ATCC 38862 \\
25 & _[PERSON]_ & 25 & _Sibilella sornic_ & ATCC 9290 \\  & & 26 & _Streptococcus agalacliae_ & STRA41 \\  & & 27 & _Vibrio cholerae_ & ATCC 1473A \\  & & 28 & _Vibrio parahaemolyticus_ & ATCC 17802 \\  & & 29 & _Vibrioulins_ & ATCC 27562 \\  & & 30 & _Yersinia enterocolitica_ & ATCC 23715 \\ \hline \hline \end{tabular}
\end{table}
Table 1: _Salmonella_ spp. tested for inclusivity and species used for exclusivity testing.  
The results showed 100% for all three parameters for all the food categories; hence, neither false-positive nor false-negative samples were detected, with this 100% performance consistent with the reference method.  
The RLOD value obtained was 1 for all the categories; therefore, the same LOD was reached both for the LAMP kit and for the reference method, while the detection limit was set at 0.4 CFU/g for all food categories (Table 3). At this concentration, false-negative results were found with the LAMP method with the commercialised kit: a sample of the heat-processed milk and dairy products and three samples of raw poultry and ready-to-cook poultry products (Table 3). For the detection limit of 0.4 CFU/g, the alternative (LAMP kit) method proved to be less sensitive than the traditional ones.