## 3 Results and Discussion
### Microarray
\begin{table}
\begin{tabular}{p{113.8 pt} p{113.8 pt} p{113.8 pt} p{113.8 pt}} \hline \hline \multicolumn{4}{c}{**Present in Tear and Plasma**} \\ \hline ADP-ribosylation Factor (ARF-6) & CNPase & IL-12 & Parathyroid Hormone-related Protein \\ \hline Alpha-fetoprotein (AFP) & Collagen I & IL-15 & Pax-5 \\ \hline Apoptosis Inducing Factor (AIF) & Collagen II & Interleukin-1 receptor associated kinase (IRAK) & PDGF alpha \\ \hline Alpha 1 Antichymotrypsin (ACT) & Collagen III & Kappa Light Chain & PDGF, beta \\ \hline Alpha Lactalbumin & Collagen IV & Keratin 19 & PLC gamma 1 \\ \hline alpha-1-antitrypsin & Cullin-3 (CUL-3) & Keratin 8 & Prohibitin \\ \hline Bcl-2-like protein 1 (bcl-XL) & Cyclin B1 & Ku (p70/p80) & Prolactin Receptor \\ \hline Beta actin & Desmin & Lambda Light Chain & pS2 \\ \hline Beta-2-Microglobulin & Epithelial Specific Antigen & Laminin B1/b1 & Rad18 \\ \hline Biotin & Ferritin & Laminin Receptor & Ras \\ \hline Chemokine receptor type 6 (CXCR6) & Fibronectin & Laminin-s & sIL-2R alpha \\ \hline Cancer Antigen 15-3 (CA15-3) & \begin{tabular}{l} Follicle Stimulating Hormone (FSH) \\ \end{tabular} & Calprotectin & \begin{tabular}{l} Silencer of Death Domain (SODD) \\ \end{tabular} \\ \hline Cancer Antigen 19-9 (CA19-9) & Follistatin & Mammaglobin B & Streptavidin \\ \hline Tyrosine-protein kinase ABL1 (c-Abl) & Gai1 & Mek2 & TGF beta Receptor III \\ \hline Cadherin-pan & Glucagon & MGMT & TGF beta1 \\ \hline Caspase 1 & \begin{tabular}{l} Glycogen Synthase Kinase 3b (GSK3b) \\ \end{tabular} & MHC II (HLA-DP and DR) & Thymidylate Synthase \\ \hline Caspase 7 (Mch 3) & \begin{tabular}{l} Granulocyte Colony \\ Stimulating Factor \\ \end{tabular} & MMP-1 & Thyroidbumin \\ \hline CD1 & \begin{tabular}{l} Glutathione S-transferase \\ (GST) \\ \end{tabular} & MMP-10 & \begin{tabular}{l} Thyroid Hormone Receptor \\ alpha \\ \end{tabular} \\ \hline CD10 & Haptoglobin & MMP-11 & TIMP-1 \\ \hline CD1b & Heat Shock Protein 27/hsp27 & MMP-2 & TR2 \\ \hline CD2 & \begin{tabular}{l} Hepatocyte Growth Factor \\ Receptor (MET) \\ \end{tabular} & MMP-2 (72 kDa Collagen & Transferrin \\ \hline CD35/CR1 & hPL & MMP-7 & Tubulin alpha \\ \hline CD40L Receptor & human Albumin & MSH2 & Tubulin gamma \\ \hline CD42b & IgA & Mucin 2 & \
\begin{tabular}{l} Uracil-DNA Glycos \\ (UNG) \\ \end{tabular} \\ \hline CD45/T200/LCA & IgA & MUTYH & VCAM-1 \\ \hline CD57 & IgG & MyoD1 & VEGFB \\ \hline Cdk1/p34 cdc2 & IgG & Neutrophil Elastase & Vitamin D Receptor (VDR) \\ \hline Cdk7 & IgM &
\begin{tabular}{l} Nitric Oxide Synthase, brain \\ (bNOS) \\ \end{tabular} & Xanthine Oxidase \\ \hline Cdk8 & IgM (m-Heavy Chain) & p130 & \\ \hline CEA/CD6ée & p19 ARF & p130 cas & \\ \hline \hline \end{tabular}
\end{table} 
Table 1: List of common proteins detected via microarray in tears and plasma samples. were involved in FGF, EGF, and JAK/STAT signaling pathways, and included proteins associated with metabolic processes and angiogenesis. Most of the proteins detected in plasma were also detected in the tear samples; however, many low molecular weight proteins showed a higher intensity in tears when compared to blood. This effect could be attributed to larger molecular weight proteins overshadowing smaller ones in plasma or due to the preprocessing leading to damage of the proteins.