Antihyperlipidemic and Antioxidant Activities of Flavonoid-Rich Extract of _Ziziphus lotus_ (L.) [PERSON]. Fruits  
[PERSON]  
1 Laboratory of Bioresources, Biotechnology, Ethnopharmacology, and Health, Faculty of Sciences, Mohammed First University, Oujda 60040, Morocco; [EMAIL_ADDRESS] (N.B.); [EMAIL_ADDRESS] (M.B.); [EMAIL_ADDRESS] (I.A.M.J.); [EMAIL_ADDRESS] (L.K.); [EMAIL_ADDRESS] (A.L.); [EMAIL_ADDRESS] (M.E.)  
[PERSON]  
1 Laboratory of Bioresources, Biotechnology, Ethnopharmacology, and Health, Faculty of Sciences, Mohammed First University, Oujda 60040, Morocco; [EMAIL_ADDRESS] (N.B.); [EMAIL_ADDRESS] (M.B.); [EMAIL_ADDRESS] (I.A.M.J.); [EMAIL_ADDRESS] (L.K.); [EMAIL_ADDRESS] (A.L.); [EMAIL_ADDRESS] (M.E.)  
[PERSON]  
5 Laboratory of Bioresources, Biotechnology, Ethnopharmacology, and Health, Faculty of Sciences, Mohammed First University, Oujda 60040, Morocco; [EMAIL_ADDRESS] (N.B.); [EMAIL_ADDRESS] (M.B.); [EMAIL_ADDRESS] (I.A.M.J.); [EMAIL_ADDRESS] (L.K.); [EMAIL_ADDRESS] (A.L.); [EMAIL_ADDRESS] (M.E.)  
[PERSON]  
6 Laboratoire de Biologie des Ligneux et des Grandes Cultures, INRAE USC1328, Campus Eure et Loir, Orleans University, 28000 Chartres, France; [EMAIL_ADDRESS] (M.E.) (M.  
several Asian countries, such as China, Iran, and South Korea [2; 3]. In Morocco, _Z. lotus_ is popularly known as \"Sedra,\" and its fruits as \"Nbeg\", and has widely distributed in arid and semi-arid areas [4]. The fruits of this plant have been used for various purposes such as nutrition, health, and thus used in cosmetics in the form of a mixture. Different parts of this plant have been traditionally used in Morocco to treat various health problems, such as cardiovascular diseases, urinary tract infections, liver disorders, gastrointestinal problems, insomnia, diarrhea, skin infections, and diabetes [4; 5; 6]. It has been reported that extracts of the fruits of _Z. lotus_ have shown considerable pharmacological properties, such as antioxidant [7], the lithophytic [4], antidiabetic, dermatoprotective [7], hepatoprotective effects [8], antispasmodic [9], anti-inflammatory, analgesic [10], antiulcerogenic [11], and gastroprotective effect [12]. Besides, this plant is well known for its safety [13]. A recently published phytochemical study showed that the presence of a total of 20 phenolic and flavonoid compounds in the alcoholic extracts of the fruits of _Z. lotus_ including (\(-\))-catechin 3-O-gallate, quercetin derivatives (such as quercetin rhamnosyl-rhammosyl glucoside, quercetin di-glucosidase, and quercetin rhamnoglucide), or eriodictyl derivatives [14]. Similarly, in the aqueous extract of the fruits of _Z. lotus_, [PERSON] and its collaborators (2019) identify twenty phenolic compounds, including catechin and rutin [7].  
Hyperlipidemia and oxidative stress have become major health concerns in recent years. These parameters are known to be the main risk factors contributing to the development and progression of atherosclerosis and associated cardiovascular and cerebrovascular diseases [15]. As a result, interest has recently increased in the use of new natural antioxidants, mainly of plant origin [16]. It is well known that flavonoids and phenolic compounds of vegetable origin have multiple pharmacological properties, particularly the antioxidant and anti-hyperlipidemic effects [17; 18; 19]. To our best knowledge, no study has been done to evaluate the antioxidant and anti-hyperlipidemia effect of the fruits of _Z. lotus_ of the Eastern Moroccan region. In this respect, the objective of our study is to assess the anti-hyperlipidemia effect of the aqueous extract of ZLF rich in flavonoids in mice fed a high-fat diet and to investigate the mechanism of this effect. Antioxidant activity was also evaluated in vitro cell-free assays.