## 3 Results and Discussion
### Identification of VOCs and nVOCs
atroviride_ TS, led to the identification of 12 molecules present in the literature, namely, diterpene lactone trichodermaerin, peptabolite asperilins A, H, and E, dicheto pyrazine methylcordsinin A, steroids 3,5,9-trihydroxygosta-7,22-dien-6-one and ergosta-7,22-dien-3-ol, sterol beta-sitosterol, adenine nucleoside, cyclopentenone atrichodermone B, sesquiterpene atrichodermone C, and the pyrone derivative 6-penta-1-enyl-pyran-2-one. The molecules identified in the extract after 10 and 30 days and their molecular structure are shown in Table 5 and in Figure 4. All the molecules known for their antimicrobial activity were recovered in the extract of the culture filtrate after 30 days with the exception of beta-sitosterol and ergosta-7,22-dien-3-ol found in the 10-day extracts. This could explain why the 30-day extracts were more active than the 10-day ones. In particular, 3,5,9-trihydroxygosta-7,22-dien-6-one was isolated from _Trichoderma_ spp. by [PERSON] et al., 2014 [44] and showed antimicrobial properties against _E. coli_, _B. subtilis_, _P. oryzae_, _C. albicans_, _A. niger_, and _A. alternata_. Beta-sitosterol isolated from _T. asperellum_[45] and _T. harzianum_[46] showed inhibitory activity against _R. solani_, _S. rolfsii_, _M. phaseolina_, and _F. oxysporum_[46]. \begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline
**Compound (nVOCs)** & **MW** & \
\begin{tabular}{c} **Molecular** \\ **Formula** \\ \end{tabular} & **m/z** & **Ion** & **RT** &
\begin{tabular}{c} **Days of** \\ **Fermentation** \\ \end{tabular} & **Reference** \\ \hline Trichodermaerin & 316.20 & C\({}_{20}\) H\({}_{28}\) O\({}_{3}\) & 317.2104 & [M+H]\({}^{+}\) & 15.9 & 30 & [47] \\ Aspereline H & 978.62 & C\({}_{47}\) H\({}_{82}\) & 977.6099 & [M-H]\({}^{-}\) & 16.7 & 10 & [48] \\ Aspereline A & 936.60 & C\({}_{45}\) H\({}_{80}\) & 935.5956 & [M-H]\({}^{-}\) & 19.3 & 10 & [48] \\ Aspereline E & 952.60 & C\({}_{45}\) H\({}_{80}\) & 951.5935 & [M-H]\({}^{-}\) & 16.6 & 10 & [48] \\ Aspereline A & 240.15 & C\({}_{12}\) H\({}_{20}\) & 299.1628 & [M+CH3 COO]\({}^{-}\) & 20.7 & 10 & [49] \\  & & N\({}_{2}\) O\({}_{3}\) & & & & & \\
3,5,9-trihydroxygosta-7,22-dien- & 444.32 & C\({}_{28}\) H\({}_{44}\) O\({}_{4}\) & 489.3224 & [M-HCOO]\({}^{-}\) & 9.4 & 30 & [44] \\ Ergosta-7,22-dien-3-ol & 398.36 & C\({}_{28}\) H\({}_{46}\) O & 397.3486 & [M-H]\({}^{-}\) & 30.3 & 10-30 & [45] \\ Beta-sitosterol & 414.39 & C\({}_{29}\) H\({}_{50}\) O & 413.3784 & [M-H]\({}^{-}\) & 33.3 & 10 & [47] \\ Adenine nucleoside & 267.10 & C\({}_{10}\) H\({}_{13}\) & 312.0953 & [M-HCOO]\({}^{-}\) & 5.5 & 30 & [45] \\ Atrichodermone B & 156.08 & C\({}_{8}\) H\({}_{12}\) O\({}_{3}\) & 215.0914 & [M+CH3 COO]\({}^{-}\) & 9.3 & 30 & [50] \\ Atrichodermone C & 236.18 & C\({}_{18}\) H\({}_{24}\) O\({}_{2}\) & 295.1906 & [M+CH3 COO]\({}^{-}\) & 23.5 & 30 & [50] \\
6-pent-1-enyl-pyran-2-one & 164.08 & C\({}_{10}\) H\({}_{12}\) O\({}_{2}\) & 165.0915 & [M+H]\({}^{+}\) & 9.9 & 30 & [51] \\ \hline \hline \end{tabular}
\end{table} 
Table 5: Nonvolatile compounds (nVOCs) in the EtOAc extracts after 10 and 30 days of incubation identified by HPLC-Q-TOF-MS. However, a few studies on the identification of nVOCs produced by _Trichoderma_ spp. are present in the literature and many more studies should be performed to understand the antimicrobial activity of the secondary metabolites produced.