## 3 Results
### Gene ontology Enrichment for Candidate Genes  
To investigate the functions of the candidate genes, we performed an enrichment analysis. First, we examined the cellular component domain and identified several interesting findings related to different cellular compartments. In 'Mitochondrial Membrane' (GO:0031966), we observed the enrichment of genes such as _DNAJC19_, _COA3_, _ABCB6_, _MRPS22_, _BNIP3_, _MRPL17_, _TIMM22_, _ATP5F1C_, _ATP5F1D_, _COQ5_, _BNIP1_, _ALDH18A1_, and _SLC25A4_, with an adjusted \(p\)-value of 0.00325. 'Organelle Inner Membrane' (GO:0019866) showed the enrichment of genes such as _DNAJC19_, _COA3_, _MRPS22_, _MRPL17_, _TIMM22_, _ALDH18A1_, _ATP5F1C_, _SIRT1_, _SLC25A4_, _ATP5F1D_, and _COQ5_, with an adjusted \(p\)-value of 0.00325. This GO term has direct descendants related to inner membranes, such as the nuclear inner membrane, mitochondrial inner membrane, and plastid inner membrane. The genes enriched in GO:0019866, excluding _SIRT1_, were also enriched in Mitochondrial Inner Membrane' (GO:0005743), with an adjusted \(p\)-value of 0.00589. Furthermore, we observed the enrichment of genes in 'Focal Adhesion' (GO:0005925), with an adjusted \(p\)-value of 0.02691, and in 'Cell-Substrate Junction' (GO:0030055), with an adjusted \(p\)-value of 0.02691, suggesting the importance of cell junction-connecting cells to the extracellular matrix. Key genes in these categories include _RPL3_, _RPS19_, _ACTN2_, _CLTC_, _ANXA5_, _CHP1_, _ILK_, _ATP6V0C_, and _FERMT2_. Additionally, the enriched genes involved in 'Preribosome, Small Subunit Precursor' (GO:0030688) were _LTV1_ and _RIOK3_, with an adjusted \(p\)-value of 0.03996. Finally, the enrichment of genes, including _DNAJC19_, _TSFM_, _POLG2_, _MRPS22_, _ATP5F1C_, _ATP5F1D_, _COQ5_, and _BCAT2_, was observed in 'Mitochondrial Matrix' (GO:0005759), with an adjusted \(p\)-value of 0.03996. The enriched GO terms are presented in Figure 3a. Focusing on the biological process, our enrichment analysis revealed a statistically significant enrichment of the GO term 'Negative Regulation of Macromolecule Biosynthetic Process' (GO:0010558), with an adjusted \(p\)-value of 0.04077. This suggests the involvement of mechanisms that decrease the rate or extent of the chemical reactions and pathways involved in macromolecule formation. The enriched genes for this term were _FXR1_, _RBM4_, _HDAC2_, _GSTP1_, _CLTC_, and _RACK1_. Additionally, we observed a significant enrichment of the GO term 'Cell-Matrix Adhesion' (GO:0007160), with an adjusted \(p\)-value of 0.0408. Figure 3: (**a**) Enriched cellular component gene ontology terms; (**b**) enriched biological process gene ontology terms; (**c**) enriched molecular function gene ontology terms. The enriched genes for this term included _ACTN2_, _ILK_, _TPPRK_, _FERMT2_, _PPFIA2_, and _BCAT2_. This term indicated that the adhesive properties by which a cell adheres to the extracellular matrix play a crucial role in cellular processes. Our analysis identified the GO term 'Translation' (GO:0006412) as significantly enriched, with an adjusted \(p\)-value of 0.04364, implying the significance of cellular metabolic processes at the level of protein formation. The key genes for this term included _TSFM_, _RPL3_, _EEF2K_, _RPS19_, _MRPS22_, _RACK1_, _RPL24_, and _MRPL17_. The GO term 'Negative Regulation Of I-kappaB kinase/NF-kappaB Signaling' (GO:0043124) showed significant enrichment, with an adjusted \(p\)-value of 0.04364, indicating the potential modulation of the I-kappaB kinase/NF-kappaB signaling pathway. The enriched genes for this GO term included _TLE1_, _RIOK3_, _GSTP1_, and _SIRT1_. A bar plot illustrating these GO terms is shown in Figure 3b. In terms of molecular function, we identified several significantly enriched terms related to protein binding and kinase activity. Particularly, 'Protein Kinase Binding' (GO:0019901) and 'Kinase Binding' (GO:0019900) exhibited highly significant enrichment, and each had adjusted \(p\)-values of 0.00319.