## 2 Results
\begin{table}
\begin{tabular}{c c c c c c} \hline \multirow{2}{*}{**pH**} & \multicolumn{2}{c}{**Time/ Duration (h)**} & \multicolumn{1}{c}{**DP**} & \multicolumn{1}{c}{**EV**} & \multicolumn{1}{c}{**MV**} & \multicolumn{1}{c}{**ANOVA**} \\  & \multicolumn{1}{c}{} & \multicolumn{1}{c}{} & \multicolumn{1}{c}{**N = 10**} & \multicolumn{1}{c}{**N = 10**} & \multicolumn{1}{c}{**N = 10**} & \multicolumn{1}{c}{_p_} \\ \hline \multirow{6}{*}{4} & 1 & 1.47 \(\pm\) 0.27  & 2.34 \(\pm\) 0.50  & 3.68 \(\pm\) 0.21  & \(<\)0.001 \\  & 2 & 3.67 \(\pm\) 1.02  & 4.18 \(\pm\) 0.78 [OTNOTE:1]Footnote 1: footnotemark: [ENDFOOTNOTE] & 6.52 \(\pm\) 0.79  & \(<\)0.001 \\  & 24 & 6.85 \(\pm\) 0.96  & 6.01 \(\pm\) 1.11  & 10.63 \(\pm\) 0.77  & \(<\)0.001 \\  & 48 & 9.24 \(\pm\) 1.11  & 7.02 \(\pm\) 1.15  & 11.23 \(\pm\) 0.80  & \(<\)0.001 \\  & 168 & 9.86 \(\pm\) 1.10  & 7.51 \(\pm\) 1.17  & 11.52 \(\pm\) 0.84  & \(<\)0.001 \\ \hline \multirow{6}{*}{5} & 1 & 1.19 \(\pm\) 0.30  & 2.43 \(\pm\) 0.51  & 2.81 \(\pm\) 0.21  & \(<\)0.001 \\  & 2 & 3.30 \(\pm\) 0.81  & 4.24 \(\pm\) 0.76  & 5.05 \(\pm\) 0.71  & \(<\)0.001 \\  & 24 & 6.61 \(\pm\) 1.63  & 5.93 \(\pm\) 1.02  & 8.43 \(\pm\) 0.70  & \(<\)0.001 \\  & 48 & 8.80 \(\pm\) 2.48  & 6.45 \(\pm\) 1.08  & 9.00 \(\pm\) 0.75  & 0.002 \\  & 168 & 9.30 \(\pm\) 2.54  & 6.83 \(\pm\) 1.10  & 9.33 \(\pm\) 0.77  & 0.003 \\ \hline \multirow{6}{*}{7} & 1 & 1.16 \(\pm\) 0.53 [FOOTNONOTE:1]Footnote 1: footnotemark: [ENDFOOTNOTE] & 2.41 \(\pm\) 0.77  & 1.64 \(\pm\) 0.20  & \(<\)0.001 \\  & 2 & 2.14 \(\pm\) 0.89  & 3.63 \(\pm\) 0.79  & 3.13 \(\pm\) 0.35 [FOOTNONOTE:1]Footnote 1: footnotemark: [ENDFOOTNOTE] & \(<\)0.001 \\  & 24 & 3.87 \(\pm\) 1.63  & 4.29 \(\pm\) 0.80  & 6.09 \(\pm\) 0.47  & \(<\)0.001 \\  & 48 & 5.05 \(\pm\) 1.69  & 4.64 \(\pm\) 0.82  & 6.31 \(\pm\) 0.43  & 0.007 \\  & 168 & 5.72 \(\pm\) 1.82  & 4.82 \(\pm\) 0.85  & 6.47 \(\pm\) 0.43  & 0.016 \\ \hline \end{tabular}
\end{table} 
Table 1: Cumulative release of fluoride from three varnishes during subsequent observation hours and results of variance analysis (ANOVA) and post hoc tests. Descriptive data were presented as mean \(\pm\) SD (\(\pm\) standard deviation). Figure 1: Cumulative fluoride release for three varnishes: Duraphat, MI Varnish and Embrace Varnish at pH = 4, pH = 5, pH = 7. Based on the obtained data for all tested varnishes in all three environments (pH = 4, 5, and 7), in the first hours, the fluoride release rate is highest, and in the hours that follow, it rapidly decreases. For MI Varnish and Embrace Varnish, the fluoride release proceeds in a similar way and is completed after about 24 h, while for Duraphat, it takes much longer (at least 48 h). The absorption measurements (see Figure 3) of all three varnishes reveals that each of them has more than 98% of light transmission in the visible and NIR regions. Differences are visible in the UV region where MI Varnish is almost transparent (above 85% of light transmission), while transmission of MI Varnish and Embrace Varnish drops to about 60% at 275 nm. Figure 3: Transmittance spectra of Duraphat, Embrace Varnish and MI Varnish in UV-VIS-NIR region. Figure 2: The course of the hourly release of fluoride in three types of varnish in three different pH environments.