## 3 Results and Discussion
### Effect of Immobilization and Freeze-drying on Cell Viability during Storage
#### 3.2.1 Storage at RT
**_plantarum_ B282**} \\  & **logcfu** & & & & **\% Survival** & & \\  & **d0** & **d40** & **d90** & **d180** & **d40** & **d90** & **d180** \\ \hline  & W & 8.6 \(\pm\) 0.2 & \textless{}1 & \textless{}1 & \textless{}1 & 0.0 \(\pm\) 0.0 & 0.0 \(\pm\) 0.0 & 0.0 \(\pm\) 0.0 \\ zea flakes & FD(ww) & 8.0 \(\pm\) 0.1 & 6.9 \(\pm\) 0.2 & 3.9 \(\pm\) 0.1 & 3.1 \(\pm\) 0.1 & 86.2 \(\pm\) 2.0 & 48.6 \(\pm\) 0.3 & 39.4 \(\pm\) 0.6 \\  & FD & 8.3 \(\pm\) 0.1 & 6.6 \(\pm\) 0.1 & 4.0 \(\pm\) 0.1 & 3.3 \(\pm\) 0.1 & 79.4 \(\pm\) 1.5 & 48.7 \(\pm\) 0.7 & 39.2 \(\pm\) 0.4 \\ pistachios & FD(ww) & 8.4 \(\pm\) 0.1 & 6.8 \(\pm\) 0.1 & 5.0 \(\pm\) 0.1 & 3.0 \(\pm\) 0.1 & 81.8 \(\pm\) 0.8 & 60.0 \(\pm\) 0.2 & 36.2 \(\pm\) 1.5 \\ pistachios & FD(ww) & 8.3 \(\pm\) 0.1 & 6.0 \(\pm\) 0.1 & 4.4 \(\pm\) 0.1 & 3.7 \(\pm\) 0.1 & 73.1 \(\pm\) 1.0 & 53.3 \(\pm\) 1.3 & 44.5 \(\pm\) 0.3 \\  & FD & 8.5 \(\pm\) 0.1 & 7.0 \(\pm\) 0.1 & 5.1 \(\pm\) 0.1 & 4.4 \(\pm\) 0.1 & 82.0 \(\pm\) 0.6 & 60.0 \(\pm\) 0.3 & 52.3 \(\pm\) 0.4 \\  & W & 8.5 \(\pm\) 0.1 & \textless{}1 & \textless{}1 & \textless{}1 & NC & NC & NC \\ raisins & FD(ww) & 7.4 \(\pm\) 0.1 & \textless{}1 & \textless{}1 & \textless{}1 & NC & NC & NC \\  & FD & 8.0 \(\pm\) 0.0 & \textless{}1 & \textless{}1 & \textless{}1 & NC & NC & NC \\  & W & 9.6 \(\pm\) 0.1 & 3.1 \(\pm\) 0.1 & \textless{}1 & \textless{}1 & 31.9 \(\pm\) 0.8 & NC & NC \\ free cells & FD(ww) & 8.4 \(\pm\) 0.1 & 7.3 \(\pm\) 0.1 & 2.7 \(\pm\) 0.1 & 1.0 \(\pm\) 0.1 & 86.3 \(\pm\) 0.7 & 32.5 \(\pm\) 0.7 & 12.3 \(\pm\) 0.7 \\  & FD & 10.8 \(\pm\) 0.1 & 8.6 \(\pm\) 0.0 & 4.0 \(\pm\) 0.0 & 2.2 \(\pm\) 0.2 & 79.7 \(\pm\) 0.3 & 37.1 \(\pm\) 0.2 & 20.0 \(\pm\) 1.8 \\ \hline \multicolumn{8}{c}{**(b) Storage at RT—P. **_acidilactici_ SK**} \\  & **logcfu** & & & & **\% Survival** & & \\  & **d0** & **d40** & **d90** & **d180** & **d40** & **d90** & **d180** \\ \hline  & W & 9.0 \(\pm\) 0.1 & \textless{}1 & \textless{}1 & \textless{}1 & NC & NC & NC \\ zea flakes & FD(ww) & 8.3 \(\pm\) 0.1 & 8.0 \(\pm\) 0.1 & 7.6 \(\pm\) 0.1 & 7.2 \(\pm\) 0.1 & 96.8 \(\pm\) 1.1 & 91.2 \(\pm\) 1.2 & 86.6 \(\pm\) 1.3 \\  & FD & 9.0 \(\pm\) 0.1 & 8.3 \(\pm\) 0.1 & 8.2 \(\pm\) 0.1 & 7.8 \(\pm\) 0.1 & 91.3 \(\pm\) 0.4 & 90.8 \(\pm\) 1.0 & 86.7 \(\pm\) 1.0 \\  & W & 9.0 \(\pm\) 0.1 & 7.0 \(\pm\) 0.1 & 6.3 \(\pm\) 0.1 & 5.8 \(\pm\) 0.1 & 76.9 \(\pm\) 2.4 & 70.3 \(\pm\) 0.5 & 64.5 \(\pm\) 0.8 \\ pistachios & FD(ww) & 8.5 \(\pm\) 0.1 & 7.8 \(\pm\) 0.1 & 7.2 \(\pm\) 0.1 & 7.0 \(\pm\) 0.1 & 91.8 \(\pm\) 1.1 & 85.4 \(\pm\) 0.9 & 82.5 \(\pm\) 0.2 \\  & FD & 9.0 \(\pm\) 0.1 & 8.1 \(\pm\) 0.1 & 7.9 \(\pm\) 0.1 & 7.6 \(\pm\) 0.1 & 90.6 \(\pm\) 1.6 & 87.8 \(\pm\) 0.8 & 84.2 \(\pm\) 0.5 \\  & W & 8.8 \(\pm\) 0.1 & \textless{}1 & \textless{}1 & \textless{}1 & NC & NC & NC \\ raisins & FD(ww) & 7.4 \(\pm\) 0.1 & \textless{}1 & \textless{}1 & \textless{}1 & NC & NC & NC \\  & FD & 8.0 \(\pm\) 0.2 & \textless{}1 & \textless{}1 & \textless{}1 & NC & NC & NC \\  & W & 9.7 \(\pm\) 0.1 & 4.9 \(\pm\) 0.1 & \textless{}1 & \textless{}1 & 50.1 \(\pm\) 0.2 & NC & NC \\ free cells & FD(ww) & 9.1 \(\pm\) 0.1 & 8.5 \(\pm\) 0.1 & 5.5 \(\pm\) 0.1 & 3.1 \(\pm\) 0.1 & 93.9 \(\pm\) 1.1 & 60.1 \(\pm\) 0.4 & 34.5 \(\pm\) 0.7 \\  & FD & 11.1 \(\pm\) 0.1 & 9.7 \(\pm\) 0.1 & 7.5 \(\pm\) 0.1 & 5.0 \(\pm\) 0.1 & 84.7 \(\pm\) 0.5 & 65.8 \(\pm\) 1.0 & 44.0 \(\pm\) 0.3 \\ \hline \multicolumn{8}{c}{**(c) Storage at RT—L.