## 1 Introduction
Moreover, norlignans, tropane alkaloids (hederacins), sesquiterpenes, sesquiterpene lactones, triterpenoids (such as unsolic and oleanolic acids), essential oils and lectins have also been identified in _Glechoma_[22; 24; 25; 26]. Literature data revealed that the biological effects of this plant include anti-melanogenic, anti-inflammatory, antibacterial, antimutagenic, genoprotective, antigenotoxic and anti-tumor effects [17; 18; 27; 28; 29]. Moreover, its antioxidant properties have been reported with respect to its use in the food industry [26; 30]. According to the literature data, _Glechoma_ is a promising source of bioactive constituents that can be beneficial in a sustainable manner,acting as natural antioxidants and antibacterial agents, but it has not been associated with phytopathogenic fungal efficacy. Extraction methods of bioactive compounds from aromatic or medicinal plants have a significant impact on the quality of the extracts and their chemical composition [12]. The literature usually describe _G. hederacea_ extracts prepared using traditional procedures such as distillation, with different parameters and solvents applied [21; 26; 31; 32]. However, these extraction methods have a number of limitations, including the fact that they are time-consuming, labor-intensive procedures that require a lot of solvents and, in some cases, produce low yields. Therefore, new potential extraction methods have emerged in recent years that provide some type of additional energy to enable the faster transfer of solutes from the sample to the solvent. Supercritical fluid extraction (SFE) is one of the alternative methods to conventional systems that has gained acceptance in the extraction of bioactive compounds from a variety of materials [33]. It is considered a clean method due to the exceptional purity of the extracts obtained. Carbon dioxide (CO\({}_{2}\)) is usually the preferred solvent in supercritical fluid extraction. It offers several advantages over other solvents, including its low cost, nonflammability, chemical inertness and lack of toxicity [33; 34]. However, polar chemicals such as phenolics cannot be extracted directly using supercritical-CO\({}_{2}\) (SC-CO\({}_{2}\)) due to the non-polarity of CO\({}_{2}\). Therefore, methanol, water or ethanol are added as co-solvents or modifiers to improve the solvation power, affinity for weakly soluble solutes (alkaloids, phenolics and glycosidic chemicals), solubility and extraction yield, depending on the operating pressure and temperature [35]. When compared to conventional separation techniques, SFE with carbon dioxide (SC-CO\({}_{2}\)) has several advantages, including physicochemical properties that are halfway between a liquid and a gas, with low viscosity, high density and diffusivity; the fact that thermally sensitive compounds can be separated at low temperatures; the solvent can be easily removed from the extracts through pressure reduction or temperature elevation; and that some studies have indicated that SC-CO\({}_{2}\) extracts have the highest antioxidant and antifungal activities [34; 35; 36; 37]. In the relation to the data mentioned above, the aim of the present work was to investigate the total phenolic content and antioxidant, antimicrobial and antibiofilm activity of _G. hederacea_ extracts obtained via supercritical dioxide extraction with methanol as the co-solvent under differential extraction conditions.