## 1 Introduction  
Medical devices and equipment are routinely used by most healthcare professionals to support and treat patients. Bioimplants are amenable to develop microbial biofilms. Biofilms are microbial communities with abilities to attach to surfaces, exhibiting high resistance to many antimicrobial agents [1; 2; 3; 4; 5]. Pathogenic microbial biofilms exert harmful effects on human health. Increased resistance of bacteria to antibiotic therapy is a major concern for medical professionals worldwide. The inherent resistance of biofilms and their pervasive involvement in implant-related infections has prompted research towards the development of antibioticfilm, antiadhesive agents. Biosurfactant (BS) producing microorganisms can disrupt biofilm on medical implants due to their antimicrobial, antibioticfilm and antiadhesive potential. Lactic acid bacteria (LAB), such as Lactobacillus constitute an important part of the natural microbiota and are recognized as potentially useful bacteria through production of biosurfactant/s (BSs) [1; 2; 6]. Reports by [PERSON] [7], [PERSON] and co-workers [8; 9; 10] suggested that Lactobacillus originated BS is highly effective against several pathogenic microorganisms with distinct antimicrobial and anti-adhesive activities. Based on structural details, different types of BSs viz. Glycolipid [5; 6], glycoprotein [8; 11], glycolipoprotein/peptide [12; 13] have been documented from _Lactobacillus_ spp. [7; 8; 11]. Lactobacillus spp. are solely known to produce cell bound/cell associated BS (CABS) and cell free BS (CFBS) [14]. BS mediated synthesis of nanoparticles (NPs) is becoming an interesting approach for many researchers [15]. Currently, several researchers are synthesizing metal NPs from microbial origin and exploiting them for biomedical purposes [16; 17]. [PERSON] et al. [18; 19] reported synthesis of silver and gold NPs by using fractionated BS (as a stabilizer) with NaBH\({}_{4}\) (as a reducing agent). BS plays a crucial role in the synthesis of gold and silver NPs from _Bacillus subtilis_ ANR 88 by growing in non-hydrocarbon, agro industrial wastes. Synthesis of uniform size and shapes silver (spherical, 4-18 nm) and gold NPs (hexagonal, 40-60 nm) from BS producing _Bacillus_ culture has been achieved [20]. Inspiring discoveries or novel formulations introduced by the engineering sector have improved our modern life. Currently incredible applications in the areas of protein crystallization, biochemical screening [21], DNA amplification, DNA sequencing and enzymatic kinetic assays [22] are driving forces to explore advanced developments of engineering. Microfluidics (MF) is one of the pioneering areas of engineering that deals with picolitre volumes of liquids [23]. MF terminology is popularly known as lab-on-a-chip (LOC) where experiments are carried out on small-scale in-vitro, mimicking in-vivo conditions [24]. MF devices have been reported to characterize cellular association in a flow system. Recently, [PERSON] et al., 2018 [25] have cultured HUVECs on the surface of synthetic micro-vascular network in a MF device (SynVivo, INC). Such study provides an opportunity to explore possible interactions of molecules with live cells. MF devices having simple and straight micro-channels may not be sufficient to replicate the complex structures of desired systems. However, to some extent MF system are helpful in exploring interactions between NP with biological molecules. Implementation of fundamental experiments, applied, analytical and diagnostic are often very challenging due to the availability of compounds in negligible quantities. MF system offers better prospects to overcome these challenges and assist medicinal and therapeutic perspectives. It is also important to highlight that the monetary inputs along with labor cost are also reduced. Automation in the experimental set up facilitates the different combinations for experimental purposes [26]. The advantage of MF set up is that the experiments can be minimized so that extremely sensitive methods can be amalgamated.