## 3 Results
### Histological Analysis of Wound Tissue  
Histological analysis of the width of the wound was indicated by yellow line an area of re-epithelialization on the wound (Figure 3). The re-epithelialization area increased on Day 7 for both DA and DAO and wounds closed occurs on Day 14 for each group. The DAO groups showed that complete re-epithelialization and faster than other groups and proved by histology on Day 7 and 14. \begin{table}
\begin{tabular}{c c c} \hline \hline
**Sample of Extracts** & **IC50 (\(\mu\)g/mL)** & **Classification** \\ \hline Sodium alginate _S. duplicatum_ & 125.31 & Moderate antioxidant \\ Okra fruit & 65.87 & Strong antioxidant \\ Alginate–okra extracts combination & 79.34 & Strong antioxidant \\ Ascorbic acid & 55.89 & Strong antioxidant \\ \hline \end{tabular}
\end{table} 
Table 1: Antioxidant activity of alginate, okra fruit and alginate–okra fruit extracts combinations (AOEs). \begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Group Treatment** & \multicolumn{4}{c}{**Blood-glucose Level (mg/dL)**} \\ \cline{2-5}  & **Day 1** & **Day 3** & **Day 7** & **Day 14** \\ \hline Normal (NC) & 111 \(\pm\) 9.9 & 110.5 \(\pm\) 7.8 & 107.5 \(\pm\) 5.0 & 120.0 \(\pm\) 14.1 \\ Diabetic (DC) & 269.5 \(\pm\) 16.3 & 252.5 \(\pm\) 19.1 & 287.5 \(\pm\) 14.9 & 220.0 \(\pm\) 24.0 \\ Alginate ointment (DA) & 251.5 \(\pm\) 7.8 & 239.5 \(\pm\) 27.6 & 229.5 \(\pm\) 7.8 & 208.0 \(\pm\) 8.5 \\ Alginate–okra ointment (DAO) & 256.5 \(\pm\) 19.1 & 169.5 \(\pm\) 24.8 & 182.5 \(\pm\) 13.4 & 115.0 \(\pm\) 19.1 * \\ \hline \hline \end{tabular}
\begin{tabular}{l c c c} \hline \hline
**Center** & \multicolumn{4}{c}{**Body-glucose Level (mg/dL)**} \\ \cline{2-5}  & **Day 1** & **Day 3** & **Day 7** & **Day 14** \\ \hline Normal (NC) & 111 \(\pm\) 9.9 & 110.5 \(\pm\) 7.8 & 107.5 \(\pm\) 5.0 & 120.0 \(\pm\) 14.1 \\ Diabetic (DC) & 269.5 \(\pm\) 16.3 & 252.5 \(\pm\) 19.1 & 287.5 \(\pm\) 14.9 & 220.0 \(\pm\) 24.0 \\ Alginate ointment (DA) & 251.5 \(\pm\) 7.8 & 239.5 \(\pm\) 27.6 & 229.5 \(\pm\) 7.8 & 208.0 \(\pm\) 8.5 \\ Alginate–okra ointment (DAO) & 256.5 \(\pm\) 19.1 & 169.5 \(\pm\) 24.8 & 182.5 \(\pm\) 13.4 & 115.0 \(\pm\) 19.1 * \\ \hline \hline \end{tabular}
\begin{tabular}{l c c c} \hline \hline
**Center** & \multicolumn{4}{c}{**Body-glucose Level (mg/dL)**} \\ \cline{2-5}  & **Day 1** & **Day 3** & **Day 7** & **Day 14** \\ \hline Normal (NC) & 111 \(\pm\) 9.9 & 110.5 \(\pm\) 7.8 & 107.5 \(\pm\) 5.0 & 120.0 \(\pm\) 14.1 \\ Diabetic (DC) & 269.5 \(\pm\) 16.3 & 252.5 \(\pm\) 19.1 & 287.5 \(\pm\) 14.9 & 220.0 \(\pm\) 24.0 \\ Alginate ointment (DA) & 251.5 \(\pm\) 7.8 & 239.5 \(\pm\) 27.6 & 229.5 \(\pm\) 7.8 & 208.0 \(\pm\) 8.5 \\ Alginate–okra ointment (DAO) & 256.5 \(\pm\) 19.1 & 169.5 \(\pm\) 24.8 & 182.5 \(\pm\) 13.4 & 115.0 \(\pm\) 19.1 * \\ \hline \hline \end{tabular}
\begin{tabular}{l c c c} \hline \hline
**Center** & \multicolumn{4}{c}{**Body-glucose Level (mg/dL)**} \\ \cline{2-5}  & **Day 1** & **Day 3** & **Day 7** & **Day 14** \\ \hline Normal (NC) & 111 \(\pm\) 9.9 & 110.5 \(\pm\) 7.8 & 107.5 \(\pm\) 5.0 & 120.0 \(\pm\) 14.1 \\ Diabetic (DC) & 269.5 \(\pm\) 16.3 & 252.5 \(\pm\) 19.1 & 287.5 \(\pm\) 14.9 & 220.0 \(\pm\) 24.0 \\ Alginate ointment (DA) & 251.5 \(\pm\) 7.8 & 239.5 \(\pm\) 27.6 & 229.5 \(\pm\) 7.8 & 208.0 \(\pm\) 8.5 \\ Alginate–okra ointment (DAO) & 256.5 \(\pm\) 19.1 & 169.5 \(\pm\) 24.8 & 182.5 \(\pm\) 13.4 & 115.0 \(\pm\) 19.1 * \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Total blood glucose of mice on Days 1, 3, 7 and 14 after treatment. Wound parameter such as neutrophils (pale green), macrophages (yellow), fibrocytes (green), fibroblasts (blue) and collagen densities (orange) showed by histology magnification of wound width (Figure 4). The number of neutrophils reaches the maximum at Day 3 while it decreases from Day 3 to Day 7 and 14 for all studied groups. The number of macrophages reaches the maximum at Day 7 while it decreased at Day 14 for all groups. The DC group was evidenced by the highest concentration of macrophages when compared to other groups.