## 4 Discussion  
The study involved a 1300-year-old canoe that researchers are trying to protect in a glass structure in a museum. However, fungal mycelia have begun to colonize the surface of the canoe due to excavation work, transportation, storage, surveys, and exhibition activities. For this reason, we analyzed the fungal flora that propagated on the wood to examine possible biodegradable fungi that might become active under new storage conditions [3]. High-throughput sequencing revealed 105 fungal genera; Basidiomycota and Ascomycota accounted for almost all the reads for each sample, and the sum of the two proportions reached 95%. Many articles reported that Ascomycota, Zygomycota, Chytridiomycota, and Rozellomycota can be found on the wooden relics deteriorated by microorganisms [25; 26; 27]. High-throughput sequencing results showed that the most abundant fungi were _Penicillium_, _Exophila_, _Cladosporium_, _Chaetomium_, _Cryptococus_, and _Aspergillus_. Although the three samples exhibited different dominant fungal genera, the above fungal genera were present in all samples. Fungal genera _Penicillium_, _Cladosporium and Trichoderma_ have been isolated from the hull and air samples using a culture-dependent approach. The high-throughput sequencing results showed that  
Figure 5: Sensitivity of fungal isolates to four biocides. The suppression halo around the paper shows the effectiveness of the biocide against the fungal strain isolates. All fungal isolates were cultured at 28 \({}^{\circ}\)C for 2 d. (**A**) NKDMZ-1. (**B**) NKDMZ-13. (**C**) NKDMZ-2. (**D**) NKDMZ-3. (**E**) NKDMZ-10. (**F**) NKDMZ-14. (**G**) NKDMZ-15. (**H**) NKDMZ-16. (**I**) NK-NH3(MH392741.1). (**J**) TJM-F5 (MH171487.1). the species composition was somewhat different from the results obtained from pure culture, probably because some fungi cannot grow on PDA or MEA medium. Many researchers have detected the genera _Penicillium_ and _Cladosporium_ in the museum environment. Indoor fungi not only exist in the form of spores in the air but also colonize various materials as mycelia. Museums are colonized by various microbes, and the detailed lists of these species vary according to the type and organization of the museum, the type of exhibit, the indoor microclimate, and the local environment [2; 28; 29; 30]. The most common colonizing fungal taxa are species of _Penicillium_, _Cladosporium_, _Alternaria_, _Aspergillus_, and _Chatonium_. [PERSON] discovered fungal contamination on a painting stored in an art museum of Tokyo. Afterwards, _Cladosporium_ and _Aspergillus_ were detected in the room where the contaminated painting was stored [31]. [PERSON] et al. investigated the fungal growth on historical textiles preserved in Slovene museums and religious institutions, using culture-dependent techniques and molecular genus-specific barcodes. The dominant contaminant fungi belonged to the genus _Penicillium_, followed by _Aspergillus_ and _Cladosporium_[32]. The genera _Penicillium_, _Aspergillus_ and _Chatonium_ have been detected on organic objects such as wooden square stools and suitcases in a museum [6]. [PERSON] et al. observed that the most important degrading component taxa were fungi of the genera _Penicillium_ and _Cladosporium_[33]. The culture-dependent identification results for the fungal aerosol samples showed that the predominant fungi in the air were _Penicillium_ sp. (47.17%) and _Cladosporium_ sp. (46.69%). In temperate climates, researchers have found that the predominant fungal spores in the air are _Cladosporium_, _Penicillium_ and _Aspergillus_. The concentration of these fungal spores varies with the season [34]. The most common fungi in the air are _Penicillium_, _Cladosporium_ and _Aspergillus_[35]. [PERSON] et al. determined microbial contamination in four museums, two libraries and two archives located in Poland. Fungal genera _Aspergillus_, _Cladosporium_, _Penicillium_, _Chatonium_, and _Trichoderma_ have been isolated from air aerosols sampled from museum storerooms [30].