## 3 Results and Discussion
### HPLC-DAD Analyses  
Due to its high extraction efficiency, the extract obtained from freeze-dried raw material was selected for qualitative analysis. Furthermore, as evident from the results of antioxidant activity analysis and total polyphenol content, lyophilization significantly influences the stability of metabolites present in the investigated plant material. Freeze-drying is the method for plant material drying allowing dried material of a much higher quality than in the case of other drying methods [28; 29]. Convection air drying is the most commonly used method but it usually takes a long time. The other disadvantage of this method apart from the long time is that the technique does not allow for the adjustment of drying parameters, which can, in some cases, lead to the degradation of valuable nutrients [30] and does not allow for the adjustment of drying parameters [30; 31; 32]. Additionally, during air drying, plants can become contaminated. The phytochemical components of _A. reptans_ extracts were determined using the HPLC-DAD method (Table 3). Among components presented in the leaf extracts, phenethyl glycosides (verbasoside, isoverbascoside), phenolic acids (chlorogenic acid, 3,4-dihydroxyphenylacetic acid, gallic acid, caffe acid, neochlorogenic acid, rosmarinic acid, ferulic acid or vanillic acid) and flavonoids (apigenin, quercetin and rutin) were detected. In the root extracts, no presence of chlorogenic acid, neochlorogenic acid, ferulic acid, apigenin, quercetin or rutin was detected (Table 3). Notable concentrations of chlorogenic acid were observed in leaves (49.75 mg/100 g DW), while its presence in roots was negligible. As for 3,4-dihydroxyphenylacetic acid, it displayed substantial variability across plant parts, with the highest concentration detected in leaves (227.65 mg/100 g DW), followed by roots (166.64 mg/100 g DW). Gallic acid, similar to chlorogenic acid, exhibited prominence in leaves (56.12 mg/100 g DW), with lower \begin{table}
\begin{tabular}{c c} \hline \hline
**Sample Name** & **Extraction Yield [\%]** \\ \hline R\_D & 18.10 \(\pm\) 2.16 \\ L\_D & 19.04 \(\pm\) 1.37 \\ R\_L & 30.13 \(\pm\) 2.07 \\ L\_L & 27.13 \(\pm\) 1.19 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Extraction yield of _A. reptans_ (L_.D—dried leaves, R_D—dried roots, L_.L—freeze-dried leaves, R_L—freeze-dried roots). concentrations observed in roots (14.17 mg/100 g DW). Minimal levels of caffeic acid were detected across both plant parts, with leaves showing higher concentration (7.26 mg/100 g DW). Neochlorogenic acid showed a relatively high level in leaves (44.76 mg/100 g DW), while its presence in roots was not detected. Among the phenolic acid compounds examined, rosmarinic acid exhibited notable concentrations in leaves (166.75 mg/100 g DW), followed by roots (163.65 mg/100 g DW). Ferulic acid was predominantly found in leaves (48.95 mg/100 g DW), with scant detection in roots. Vanillic acid had its highest concentration in leaves (19.83 mg/100 g DW), and slightly lower in roots (3.57 mg/100 g DW). Flavonoids (apigenin, quercetin, and rutin) were significantly present in leaves, while their presence in roots was minimal. Leaves exhibited the highest concentrations of both verbascoside and isoverbascoside, with notably lower levels detected in roots. Comparing the obtained results to the literature data, the extraction conditions applied allow for obtaining extracts richer in polyphenolic compounds. In the study by [PERSON] et al. (2019), ethanolic extracts derived from the aerial parts of _A. reptans_ were assessed for total polyphenol content (19.81 mg GAE/g DW) and antioxidant activity using the DPPH method (42.75 mg/mL) and TEAC assay (60.98 mg/100 g DW). Our HPLC analysis revealed superior concentrations of rutin (51.18 mg/100 g DW), quercetin (71.55 mg/100 g DW), apigenin (99.26 mg/100 g DW), and ferulic acid (48.95 mg/100 g DW) in the leaf extracts [18]. The HPLC analysis results, revealing diverse phenolic compound contents across different parts of _A.