# Comprehensive Analysis of the Acute Toxicity of Ionic Liquids Using Microtax(r) Bioassays
## 3 Results and Discussion
\begin{table}
\begin{tabular}{c c c c} \hline
**IL** & **EC\({}_{\bf 50}\) 5 min/mg/L** & **EC\({}_{\bf 50}\) 15 min/mg/L** & **EC\({}_{\bf 50}\) 30 min/mg/L** \\  & **(Lower; Upper) Limits** & **(Lower; Upper) Limits** & **(Lower; Upper) Limits** \\ \hline C\({}_{2}\)C\({}_{1}\)Im TFSI & 367.43 (247.06; 487.80) & 189.97 (104.68; 275.27) & 113.08 (41.74; 184.42) \\ C\({}_{4}\)C\({}_{1}\)Im TFSI & 78.48 (36.48; 120.48) & 54.85 (24.91; 84.79) & 46.58 (14.68; 78.48) \\ C\({}_{4}\)C\({}_{1}\)C\({}_{1}\)Im TFSI [12] & 150.44 (72.43; 228.49) & 113.32 (82.29; 144.35) & 98.70 (82.39; 115.01) \\ C\({}_{6}\)C\({}_{1}\)Im TFSI & 26.29 (24.00; 28.57) & 23.64 (21.83; 25.45) & 29.60 (27.66; 31.53) \\ C\({}_{8}\)C\({}_{1}\)Im TFSI & 3.57 (3.43; 3.71) & 4.23 (3.86; 4.59) & 5.97 (4.85; 7.10) \\ AC\({}_{1}\)Im TFSI & 655.63 (417.77; 983.49) & 337.68 (235.35; 440.01) & 249.30 (175.09; 323.52) \\ AC\({}_{1}\)Im CI & 1399.39 (463.05; 2335.73) & 842.87 (531.77; 1153.97) & 715.21 (479.39; 951.03) \\ AC\({}_{1}\)Im DCA & 1181.58 (866.64; 1496.51) & 639.62 (538.85; 740.39) & 534.19 (454.01; 614.38) \\ C\({}_{4}\)C\({}_{1}\)Im FAP & 97.64 (70.21; 125.1) & 77.43 (59.59; 95.27) & 74.37 (58.05; 90.69) \\ C\({}_{1}\)C\({}_{1}\)Im DMP & 1186.31 (1071.08; 1300.92) & 1198.33 (1111.72; 1285.07) & 1254.24 (1172.95; 1337.75) \\ C\({}_{2}\)Py TFSI & 314.24 (175.08; 453.40) & 133.72 (54.34; 213.11) & 74.31 (0.00; 150.51) \\ C\({}_{4}\)Py TFSI & 150.21 (133.75; 166.66) & 106.84 (93.51; 120.17) & 92.90 (78.26; 107.54) \\ C\({}_{6}\)Py TFSI & 44.16 (40.40; 47.92) & 40.30 (36.29; 44.30) & 45.84 (41.92; 49.76) \\ C\({}_{5}\)C\({}_{1}\)Piry TFSI & 215.24 (161.47; 269.01) & 138.13 (104.77; 171.49) & 117.07 (82.16; 151.98) \\ C\({}_{4}\)C\({}_{1}\)Piry TFSI & 150.63 (140.30; 160.95) & 119.23 (110.40; 128.07) & 107.37 (101.19; 113.56) \\ C\({}_{4}\)C\({}_{1}\)Pyrr TFSI [12] & 1463.91 (1162.13; 1765.69) & 964.58(791.32; 1137.88) & 714.43 (577.92; 851.21) \\ C\({}_{6}\)C\({}_{1}\)Pyrr TFSI & 88.95 (79.22; 98.69) & 70.29 (62.57; 78.00) & 75.26 (66.25; 84.27) \\ C\({}_{8}\)C\({}_{1}\)Pyrr TFSI & 15.71 (13.10; 18.31) & 15.80 (13.03; 18.57) & 23.30 (16.95; 30.25) \\ C\({}_{4}\)C\({}_{1}\)Pyrr FAP & 805.87 (554.86; 1056.83) & 707.70 (562.28; 853.11) & 604.90 (516.64; 693.16) \\ C\({}_{1}\)OC\({}_{2}\)C\({}_{1}\)Pyrr FAP & 62.37 (31.43; 93.31) & 37.94 (23.69; 52.19) & 31.59 (22.00; 41.19) \\ C\({}_{4}\)C\({}_{1}\)Pyrr C\({}_{4}\)FAP & 96.75 (71.83; 121.66) & 62.28 (36.83; 87.74) & 50.54 (22.51; 78.57) \\ C\({}_{4}\)C\({}_{1}\)Pyrr C\({}_{8}\)PAP & - & - & 5430.07 (1854.01; 2242.73) \\ Phy FAF & 3555.25 (2429.44; 4605.18) & 1096.36 (569.37; 1623.35) & 805.63 (417.84; 1193.42) \\ EAN [12] & 12,582.07 (8186.64; 16977.50) & 10,665.47 (6650.14; 14680.80) & 9711.63 (6561.46; 12860.79) \\ PAN [23] & 8314.99 (7268.61; 9361.37) & 5932.88 (5043.45; 6822.30) & 5827.78 (4998.72; 6656.84) \\ BAN [23] & 1491.99 (636.69; 2347.04) & 1066.71 (551.52; 1581.90) & 1017.14 (478.49; 1555.78) \\ PEAN [23] & 1116.9 (945.1; 1288.8) & 1073.6 (836.3; 1311.0) & 1029.8 (792.5; 1267.1) \\ HEAN [23] & 85.69 (77.71; 93.68) & 57.54 (52.98; 62.10) & 50.12 (44.85; 55.39) \\ OAN [23] & 9.70 (6.37; 13.03) & 7.33 (5.23; 9.43) & 7.38 (5.51; 9.25) \\ C\({}_{2}\)Im NO\({}_{3}\)[12] & 612.55 (395.90; 82.01) & 573.77 (372.29; 774.55) & 597.89 (408.00; 785.08) \\ \hline \end{tabular}
\end{table} 
Table 2: EC\({}_{50}\) effective concentration values in mg/L and the respective 95% confidence intervals, obtained after 5, 15, and 30 min of exposure of the marine bacteria _A. fischeri_. Figure 4 shows the values of EC\({}_{50}\) after 15 min of exposure for IL with common ions: anion FAP (a), NO\({}_{3}\) (c), and TFSI (e), and cation ammonium (b), pyrrolidinium (d), and imidazolium (f). From this figure, it is easy to conclude that the increase in the alkyl chain leads to an increase in toxicity. An important finding of this work corresponds to the fact that the reduction in EC\({}_{50}\) with the increase in the length of the alkyl chain is especially drastic and significant when C = 6 is achieved.