## 1 Introduction
The detailed analysis of antifungal activity of various compounds against yeast cells is, therefore, an easy and reliable approach for a first idea for the understanding of other toxic properties, such as anticancer activities. To understand the mode of action and to characterize the promising target of cytotoxic compounds, the technique called chemogenetic profiling is exploited. This procedure employs the screening of mutant yeast strains with knocked out genes encoding specific proteins [21]. In the presence of a cytotoxic compound, the heterozygous diploid strain with gene deletion and the wildtype homozygous diploid yeast strain are allowed to grow, and subsequently, the growth inhibition shown by yeast strains when compared with the wildtype, points towards the drug target [22]. In response to the exposure to a cytotoxic compound, a library of diploid yeast strains devoid of one copy of different genes, leads to approximately partially expressed protein, and this technique refers to the haplonsufficiency. While interpreting the compound's unknown mechanism of action (MOA), the haplonsufficiency screening technique identifies the cellular targets, as well as the off-target mode of action (_e.g._, neuroleptic agents) [23]. Exploring the specific mode of action of various compounds represents a crucial task in the drug discovery and development. Yeast deletion collection comprising 21,000 haploid and 6000 diploid deletion strains of _Saccharomyces cerevisiae_ is useful for identifying the mechanism of action and drug discovery [24]. The yeast, _S. cerevisiae_, is commonly employed and the reason to opt for it as a model organism is because of the good characterization of its genome and proteome, with approximately 45% gene homology with mammalian genes [23]. Yeast is a eukaryote and, hence, has a nucleus and other organelles, such as mitochondria, endoplasmic reticulum and vacuoles like in animals and plants. Yeast is more closely related to animals than plants at the molecular level [25]. Many pathways are conserved in evolution from yeast to mammals [26]. Yeast is also a good model for the molecular understanding of human diseases [27; 28]. Approximately, 1200 mutants of heterozygous diploid yeast strains are available for scientific community from Euroscarf [29]. The present study involves the investigation of the role of glutathione and oxidative stress as the antifungal mode of action of selenocyanates by employing a combination of various assays. Chemogenetic profiling was carried out by utilizing a specific yeast mutant library deficient in genes coding for redox related protein function to confirm the specific role of glutathione [30]. In this study, we focused mainly on the redox-function of the selenocyanates, thus, we chose a sublibrary including the mutant strains compromised in genes related to redox-maintenance. The protective role of glutathione against selenocyanates was assessed by employing _Arabidopsis_ seedling root growth assay. _Arabidopsis_ is a multicellular organism and responds very sensitively to changes in glutathione homeostasis. This property makes it a suitable system to analyze the effects of selenocyanates on glutathione pool. The glutathione level determination in yeast also provided a glimpse into the antifungal mode of action of organoselenocyanates.