## 4 Results and Discussion  
### Biological validation of extracted Key Genes  
By mining the KEGG pathways [35] and NCBI Entrez system, the biological interpretations of the extracted key genes are listed in Tables 1-4. Each table contains the following details about the extracted key genes: the affy ID, gene symbol, Entrez ID, oncology, and the gene pathway. \begin{table}
\begin{tabular}{c c c c c} \hline
**AFFY ID** & **Gene Symbol** & **ENTREZ Gene ID** & **Oncology** & **Gene Pathway** \\ \hline
201010\_s\_at, & & & & Breast cancer, prostate Carcinoma, & REACT\_75808. The NLPF3 inflammasomeome. \\
201008\_s\_at, & TXNIP & 10628 & colorectal carcinoma, Hepatocellular & cellular response to tumor cell \\
201009\_s\_at & & & & Carcinoma (HCC) \\ \hline
203438\_at, & & & & Colorectal cancer, Breast cancer, & \\
203439\_s\_at & & & & Mutation of HCC & KEGG: hsa: 8614. \\ \hline
205047\_s\_at & ASNS & 440 & Cancer, & & REACT\_238. liver development. \\  & & & & Protein and/or amino acid deprivation & REACT\_18355. \\ \hline
202887\_s\_at & DDIT4 & 54541 & Pancreatic tumor, prostate cancer, lung carcinoma & REACT\_35377. \\ \hline
219270\_at & CHAC1 & 79094 & downstream of the ATF4 & TF53 Regulates Metabolic Genes \\ \hline
206085\_s\_at, & & & & KEGG: hsa?9094 \\
217127\_at & CTH & 1491 & Bladder Cancer & CHAC1 is a component of the UPR, \\  & & & & unfolded protein response pathway. \\ \hline
206085\_s\_at, & & & & \\
217127\_at & CTH & 1491 & Bladder Cancer & REACT\_115859. \\  & & & & Cysteine omation from homocysteine \\ \hline
1556499\_s\_at & COL1A1 & 1277 & Mutation in liver, infinative skin & REACT\_118779. \\  & & & carcinoma, bendnar carcinoma & Extracellular matrix organization. cascade. \\ \hline
210587\_at & INHBE & 83729 & hepatocellular carcinoma, HCC & REACT\_15398. Glycoprotein hormones \\ \hline
202672\_s\_at & ATF3 & 467 & Solid tumor & REACT\_18355. ATF4 activates genes. \\ \hline \end{tabular}
\end{table} 
Table 1: Functional annotation of key genes detected using \(T\)-test, and F-test methods after different periods of post-infection with C virus. \begin{table}
\begin{tabular}{c c c c c} \hline
**AFFY ID** & **Gene Symbol** & **ENTREZ Gene ID** & **Onocology** & **Gene Pathway** \\ \hline
213322\_at & OARD1 & 221443 & infiltrating duct carcinoma & KEGG: hsa: 221443. \\ \hline
36711\_at & MAFF & 23764 & leukemia/lymphoma (BCR-ABL1) & REACT\_24970. megakaryocyte, and platelet construction. \\ \hline
205749\_at & CYP1A1 & 1543. & hepatocellular carcinoma, NOS, & KEGG: hsa: 1543. \\  & & unstated behavior & REACT\_116145. PPAR activates gene \\  & & & expression. \\ \hline
219371\_s\_at & KLF2 & 10365. & chronic lymphocytic B-cell leukemia & KEGG: hsa: 10365. \\ \hline
212558\_at & SPRY1 & 10252 & HCC, gastrointestinal stromal sarcoma & REACT\_12484. EGFR downregulation. \\ \hline
205047\_s\_at & ASNS & 440 & Cancer, & REACT\_238. \\  & & & Protein and/or amino acid deprivation & liver development. \\  & & & Protein and/or amino acid deprivation & REACT\_18355. \\ \hline
201010\_s\_at & & & & \\
201009\_s\_at & TXNIP & 10628 & HCC, Breast cancer, prostate & REACT\_75808. The NLRP? inflammasome. \\  & & & Carcinoma, colorectal carcinoma & cellular response to tumor cell \\ \hline
203119\_at & CCDC86 & 79080 & HCV, squamous cell carcinoma & KEGG: hsa?9080 \\ \hline
232780\_s\_at & ZNF691 & 51058 & Infiltrating duct carcinoma & REACT\_12627. Generic Transcription Pathway. \\ \hline
202847\_at & PCK2 & 5106 & HCC & KEGG: HSA00020 Citrate cycle (TCA cycle) \\ \hline \end{tabular}
\end{table} 
Table 2: Functional annotation of key genes detected using cosine coefficient and Pearson’s correlation methods after different periods post-infection with C virus.