# Effect of Mechanical Damage in Green-Making Process on Aroma of Rougui Tea  
[PERSON]  
1 College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China; [EMAIL_ADDRESS] (F.L. ): [EMAIL_ADDRESS] (H.W. ); [EMAIL_ADDRESS] (C.G. ); [EMAIL_ADDRESS] (Z.W)  
[PERSON]  
1 College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China; [EMAIL_ADDRESS] (F.L. ): [EMAIL_ADDRESS] (H.W. ); [EMAIL_ADDRESS] (C.G. ); [EMAIL_ADDRESS] (Z.W)  
[PERSON]  
3 Institute of Animal Husbandy and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China; [EMAIL_ADDRESS] 3  
[PERSON]  
2 Anxi College of Tea Science, Fujian Agriculture and Forestry University, Quanzhou 362406, China; [EMAIL_ADDRESS] 2  
[PERSON]  
4 Fuding Tea Technology Promotion Station, Ningde 355200, China; [EMAIL_ADDRESS] 4  
[PERSON]  
1 College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China; [EMAIL_ADDRESS] (F.L. ): [EMAIL_ADDRESS] (H.W. ); [EMAIL_ADDRESS] (C.G. ); [EMAIL_ADDRESS] (Z.W)  
[PERSON]  
1 College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China; [EMAIL_ADDRESS] (F.L. ): [EMAIL_ADDRESS] (H.W. ); [EMAIL_ADDRESS] (C.G. ); [EMAIL_ADDRESS] (Z.W)  
[PERSON]  
5 Faa Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China  
5 Faa Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 3  
are obviously increased during green-making, and a large number of volatile compounds containing nitrogen are formed after baking [1; 2]. RGT produces a large number of alcohols and aldehydes during fermentation, such as linalool, indole, nerol, (Z)-3-hexen-1-ol and (Z)-3-hexenyl acetate [3; 4; 5]. Other WRTs produce similar volatile substances, such as terpene, aldehyde and ester compounds, during the manufacturing process, and green-making has a significant effect on it, finally promoting the formation of flowery or fruity aroma characteristics [6; 7; 8]. During the manufacturing process of WRT, the volatile content that mainly contributes to the grassy odor decreases, while the volatile content that mainly contributes to the flowery and fruity aroma increases [9]. After picking the fresh leaves of RGT, they are withered in sunlight for a short time and then subjected to green-making and the subsequent manufacturing process, which is more conducive to producing a mellow taste and flowery aroma. Sun withering can increase the gene expression of ubiquinone, other terpenoid quinones' biosynthesis, pyruvate metabolism and the starch and sucrose metabolism pathway, which increases the content of terpenoids, organic acids and lipids, thus enhancing the mellow taste and flowery aroma of the tea [10]. Different withering degrees also affect the quality of RGT. A previous study found that moderate withering (a withering time of 30 min) could increase the content of terpenoids, organic acids and lipids in the leaves and improve the quality of the RGT [11]. It can be seen that withering affects RGT's quality, but the effect of green-making on RGT's quality is the most significant. Studies have shown that the biosynthesis and release of volatile organic compounds (VOCs) in plants are related to self-defense in the face of biotic and abiotic stresses [12]. Green-making causes the continuous mechanical injury and dehydration of the fresh tea leaves, which induces the transformation and release of volatile organic compounds under traumatic stress. Biotic stress and abiotic stress during the tea manufacturing process promote the formation of various aroma compounds, which has become a major research topic in recent years [13]. Quantitative real-time PCR (qRT-PCR) and transcriptome data showed that many genes related to the formation of flowery and fruity aromas were activated during green-making [14; 15; 16]. The content of indole, jasmine lactone and (E)-nerolidol in fresh tea leaves during green-making was positively related to the expression of the CsTSB2, CsTSA, CsLOX1 and CsNES genes [17; 18; 19].