## 1 Introduction  
From antibacterial properties to UV radiation protection, bioactive films are used in a wide range of applications [1, 2], including for the antioxidant properties by supplementing compounds such as naphtho-\(\gamma\)-pyrone in film-forming solutions (FFSs) based on yeast cell walls [3] to the use of edible films as carriers of bioactive compounds [4] or the incorporation of lactic acid bacteria (LAB) producing bacteriocins into edible whey protein films for antimicrobial properties [5] and up to product packaging materials that dissolves into the oral solution formed with the product [6, 7]. A new possibility for the development of bioactive films is the incorporation of different types of fruit juices into the film-forming solution to impart antioxidant and antimicrobial properties [8, 9]. Suitable matrices of FFS can be represented by yeast cell walls [3, 10] or \(\beta\)-glucas extracted by purification from the cell walls, with immunostimulatory effect. The bioactivity of films developed from juices, compared to edible films, consists mainly of the high content of tannins and polyphenols such as in the following: pomernate juice (_Punica granatum_ L.) pilediannins between 2010 and 6420 mg/L (up to 103 mg/L elastic acid) and gallic acid up to 8.55 mg/L [11, 12, 13]; up to a 444.5 mg/100 g of anthocyanins in bilberry juice (_Vaccinium myrtillus_ L.) and 65 mg/100 mL flavonols [14, 15]; or about 59 compounds identified by [PERSON] et al., (2022) in ingenberry (_Vaccinium viitis-idae_ L.) including quercetin, catechins, flavonoids, and a phenolic content up to a total of 760 mg/100 g fresh weight [16, 17]. With the synergistic action of pomernate, bilberry, and cranberry juices, along with \(\beta\)-glucas from yeast, it confers antioxidant and antidiabetic properties, as well as immunomodulatory activity [17, 18, 19, 20, 21]. For example, in bilberries, it has been found that due to anthocyanins, in case of vascular complications the fruit is used instead of leaves. For a daily intake of 25% anthocyanins from the fruit extract, doses of up to 240-480 mg per day have been established [22]. In the case of cranberries, a consumption of 240 mL cranberry juice (45.7 mg polyphenols) daily for a period of 12 weeks was beneficial in the control of type II diabetes [21]. Vascular functions can also be visibly improved after a month of consumption of cranberry due to the high content of polyphenols (especially proanthocyanidins type A, anthocyanins, flavonoids, and phenolic acids) [23]. Besides bilberry-derived bioactive compounds with hypoglycemic activity, pomegranate juice is also particularly valuable and can be used in the management of diabetes complications from vasoprotective effect to reduction in serum glucose levels. In a communication on how pomegranate juice acts on glucose metabolism, [PERSON] et al. (2022) summarized in a table up to 10 case reports on the effect of pomegranate juice in type II diabetes. These includes blood glucose reduction after consuming 200 mL for 4 days while another report revealed that after 4 weeks the PON1, an HDL-associated enzyme increased, helping to reduce total cholesterol [24]. Films developed from pomegranate extract, in addition to the antioxidant and antimicrobial activity, were shown to improve mechanical properties, thermal stability, and inhibited the activity of _Penicillium digitatum_[25]. \(\beta\)-glucans are described as high molecular weight polymers of glucose that can be isolated from oats, plants, bacteria, fungi, and yeast [26]. Depending on the source of origin, they can play roles ranging from dietary fiber (as in the case of \(\beta\)-glucans from oats) to anti-cancer, anti-inflammatory, and immune system stimulating roles (\(\beta\)-glucans from mushrooms and yeast) [27]. The benefits of using \(\beta\)-glucans isolated from yeast cells can be attributed to the ability to stimulate both the innate and acquired immune systems [28; 29].