###### Abstract
The efficiency of PEF to permeabilize cell membranes differs according to process parameters such as electric field strength, treatment time, specific energy, pulse shape, pulse width, frequency, temperature, the properties of the treated food sample such as its pH and conductivity, and the characteristics of the target cells [19]. An example of its usefulness is observed in the extraction of lipids from microalgae. In this regard, a first PEF-assisted extraction of water-soluble bioactive compounds that cross the membrane through the formed micropores can be performed and then a second extraction with ethanol (EtOH) can be applied to obtain lipids or lipid-soluble metabolites that have remained inside the cell, which provides advantages in subsequent purification processing [20]. The use of PEF is possible in aqueous solutions with a low dry matter content, facilitating the more energy-efficient isolation of high-added-value compounds extracted from microalgae directly from the culture without dehydration or drying [20; 21]. Most of the studies evaluating the application of PEF focus on the use of aqueous suspensions of microalgae, which mainly allows for the extraction of water-soluble compounds [22; 23], while the extraction of non-polar pigments (e.g., chlorophylls and carotenoids) is very low under these conditions due to their low solubility in water [24]. Some previous studies have evaluated the use of complementary extractions with EtOH (96%), obtaining higher yields in the extraction of pigments from microalgae pre-treated with PEF [25]. Studies combining the use of PEF in suspensions of microalgae (_Nanochloropsis_) in water and the subsequent addition of an organic solvent to improve pigment extraction efficiency have been also carried out [12; 13]. PEF-assisted extraction yields of nutritionally valuable compounds (lipids, pigments, and proteins) can vary depending on the microalgae used [26]. Therefore, specific studies are needed to evaluate the extraction in different microalgae species at different times and with different solvents to obtain the necessary information and thus be able to scale up the process to an industrial level in an efficient, sustainable way and with a high extraction yield. Several studies are currently evaluating PEF-assisted extraction from microalgae biomass such as Chlorella and _Nannochloropsis_[12; 22; 25; 27]. However, there is a lack of data on the impact of PEF on the extraction of high-added-value compounds from other microalgae species such as spirulina (_Arthospira platensis_). Spirulina is an undifferentiated filamentous cyanobacterium [28; 29] whose cells are 3-12 um wide and can reach 16 um [30]; it has been used as food for centuries by various cultures as it has a biochemical profile rich in bioactive molecules and there are studies that support its benefits for human health [31]. However, considering the gap that exists regarding different extraction levels of antioxidant compounds according to the different microalgae species, the main aim of this study was to evaluate how PEF pre-treatment combined with the binary mixtures of ethanol (EtOH)/H2O, 50:50, \(v/v\) and dimethyl sulfoxide (DMSO)/H2O, 50:50, \(v/v\) at different extraction times can affect to the recovery of antioxidant bioactive compounds from spirulina.