## 3 Results
### Effects of FSH-ZM on Wrinkle Formation and Skin Thickness in UVB-Irradiated HRM-2 Mice  
Wrinkle formation was significantly increased in the C group compared with that in the NC group and significantly decreased in the PC1 and PC2 groups compared to the C group. Compared with that in the C group, the groups receiving the diet containing Z showed significantly decreased wrinkle formation in a dose-dependent manner (Figure 3A). Skin thickness was significantly increased in the C group compared with that in the NC group and significantly decreased in the PC1 and PC2 groups compared to the C group. Compared with that in the C group, the groups receiving the diet containing Z showed significantly decreased skin thickness in a dose-dependent manner (Figure 3B). Effects of FSH-ZM on mRNA and Protein Expressions Related to Wrinkle Formation in UVB-Irradiated HRM-2 Mice  
The mRNA or protein expressions of the MAPK8, AP-1, MMP1, MMP3, and MMP9 were significantly increased in the C group compared with that in the NC group and significantly decreased in the PC1 and PC2 groups compared to the C group. In comparison with the C group, the groups receiving the diet containing Z decreased the mRNA or protein expression of the MAPK8, AP-1, MMP1, MMP3, and MMP9 in a dose-dependent manner (\(p\) < 0.05). On the other hand, the mRNA or protein expression of procollagen and collagen type I (COL1A1) were significantly decreased in the C group compared with that in the NC  
Figure 2: Effects of FSH-ZM on the expression of mRNAs related to skin moisturizing and skin hydration content in skin from the back of UVB-irradiated HRM-2 hairless mice. (**A**) The expression of HAS2 mRNA. (**B**) The expression of Fibrillin-1 mRNA. (**C**) The expression of Elastin mRNA. (**D**) Skin hydration. NC, normal control; C, UVB-irradiated control; PC1, positive control 1, L-ascorbic acid 200 mg/kg b.w. ; PC2, positive control 2, Arbutin 200 mg/kg b.w. ; Z100, FSH-ZM 100 mg/kg b.w. ; Z200, FSH-ZM 200 mg/kg b.w. Values are presented as mean \(\pm\) standard deviation (n = 6), and different superscript letters indicate significance at \(p\) < 0.05.  
group and significantly increased in the PC1 group compared to the C group. Compared with that in the C group, the groups receiving the diet containing Z increased the mRNA or protein expression of the procollagen and collagen type I (COL1A1) in a dose-dependent manner (\(\rho<0.05\); Figure 4). Figure 4: Inhibitory effects of FSH-ZM on mRNA and protein expression related to wrinkle formation in skin from the back of UVB-irradiated HRM-2 hairless mice. (**A**) The mechanism of wrinkle formation. (**B**) The expression of MAPK8 mRNA. (**C**) The expression of AP-1 mRNA. (**D**) The expression of MMP1 mRNA. (**E**) The expression of MMP3 mRNA. (**F**) The expression of MMP9 mRNA. (**G**) The expression of Procollagen mRNA. (**H**) The expression of Collagen type I mRNA. (**I**) Protein expressions. NC, normal control; C, UVB-irradiated control; PC1, positive control 1, L-ascorbic acid 200 mg/kg b.w. ; PC2, positive control 2, Arbutin 200 mg/kg b.w. ; Z100, FSH-ZM 100 mg/kg b.w. ; Z200, FSH-ZM 200 mg/kg b.w. Values are presented as mean \(\pm\) standard deviation (n = 6), and different superscript letters indicate significance at \(p<0.05\). Figure 3: Effects of FSH-ZM on wrinkle suppression and skin thickening in skin from the back of UVB-irradiated HRM-2 hairless mice. (**A**) The wrinkle photography. (**B**) H&E staining. (**C**) Fontana–Masson staining. NC, normal control; C, UVB-irradiated control; PC1, positive control 1, L-ascorbic acid 200 mg/kg b.w. ; PC2, positive control 2, Arbutin 200 mg/kg b.w. ; Z100, FSH-ZM 100 mg/kg b.w. ; Z200, FSH-ZM 200 mg/kg b.w. Effects of FSH-ZM on mRNA Expressions Related to Pro-Inflammatory Cytokines in UVB-Irradiated HRM-2 Mice  
The mRNA expressions of the IL-1\(\beta\), IL-6, and TNF-\(\alpha\) were significantly increased in the C group compared with that in the NC group and significantly decreased in the PC1 group compared with that in the C group.