###### Abstract  
Leakages of CO\({}_{2}\) capture and storage systems from the seabed are able to cause significant adverse biological effects in marine species. Adult mussels were exposed to different CO\({}_{2}\) enrichment scenarios (pH from 8.3 to 6.0) for 96 h, and endpoints (lysosomal membrane deterioration, lipid peroxidation and primary damages in DNA) were assessed. Mortality and reduced health status can occur after short exposure of the tropical mussel _Perna perna_ to pH levels lower than 7.5. Results pointed out cytogenetic effects in the hemolymph and gills after 48 and 96 h of exposure, respectively. These findings should be considered when environmental monitoring approaches are performed in tropical marine areas employing CCS strategies. CO\({}_{2}\) enrichment; mollusks; ocean acidification; biomarkers; carbon capture storage Article  
[PERSON], [PERSON], [PERSON], L.F.; [PERSON], [PERSON], [PERSON], C.D.S. Integrated Assessment of CO\({}_{2}\)-Induced Acidification Lethal and Sub-Lethal Effects on Tropical Mussels _Perna perna_  
[PERSON], [PERSON], [PERSON], L.F.; [PERSON], [PERSON], [PERSON], C.D.S. Integrated Assessment of CO\({}_{2}\)-Induced Acidification Lethalhal and Sub-Lethal Effects on Tropical Mussels _Perna perna_the timeframe for safe CO\({}_{2}\) storage being relatively unknown [15]. Considering that oceans have the largest capacity for CO\({}_{2}\) storage, sub-seabed geological formations, such as depleted oil and gas reservoirs and saline aquifers, have been designated as potential storage locations for CO\({}_{2}\) sequestration. The complexity in foreseeing the location and magnitude of possible seepage makes difficult the evaluation of potential effects on aquatics ecosystems. Two main potential sources of CO\({}_{2}\) escape are transport facilities and storage areas [16]. The effects of CO\({}_{2}\) leakage will depend on the amount and/or rate of leakage, the transport, the dispersion processes and the chemical buffering capacity of the sedimentary or water system, contributing to the imbalance of seawater's chemistry. There are also natural sources of CO\({}_{2}\) enrichment, such as natural CO\({}_{2}\) vents [17; 18], bacterial organic matter degradation, diagenesis process [19] and submarine eruptions such as in the Canary Islands (Spain), where pH values between 5.13 and 8.04 have been measured [20; 21]. In this context, many studies have been performed in order to assess the impacts of changes in the marine carbonate system as well as pH reduction via CO\({}_{2}\) enrichment to organisms [22; 23; 24; 25; 26]. Several authors mimicked a CO\({}_{2}\) leakage in the lab, assessing different responses related to decreases in pH values in diversified organisms, for instance: growth rate of bacteria (_Roseobacter_ sp. CECT 7117 and _Pseudomonas litoralis_ CECT 7670) [27] and bacterial communities [28]; mortality rate applied to amphipods (_Ampelisca brevicornis_ and _Hyale youngi_) [29]; impact on the early life stages of marine mussel _Perna perna_[30; 31]; and effects on growth, cell viability and oxidative stress using three microalgae species (_Tetraselmis chuii_, _Phaedactylum tricornutum_ and _Nunnochloropsis gaditan_) [32]. Histopathological effects and lysosomal membrane stability were also assessed in mollusks such as claims (_Ruditapes philipinarum_) and mussels (_Mytilus edulis_) [33; 34] apart from a battery of biomarkers using the clam _Scrobicularia plana_[35]. Ocean acidification impacts other aspects of a marine organism's physiology, including acid-base balance, energy metabolism, redox balance and behavior [36; 37]. Acidification of ocean surface water is a currently developing scenario that warrants a broadening of research foci in the study of acid-base physiology, ensuring a strong basis for the physiological interactions of ocean acidification with pollutants that may affect the same molecular and physiological pathways [38].