## 3 Results and Discussion  
### Amino Acid Contents of Major Ingredients (Laver and Surimi)  
The results of the amino acid composition of the layer and surimi are shown in Table 2. The total amino acid content of laver was 31,610 mg/100 g, of which the essential and non-essential amino acid contents were 11,293 mg/100 g (35.73%) and 20,316 mg/100 g (64.27%),respectively, suggesting that it is an excellent source of protein. In addition, six essential amino acids, including lysine, threonine, leucine, isoleucine, valine, and phenylalanine, were detected. Among them, leucine, valine, and isoleucine, which are widely known as BCAA (branched-chain amino acids), accounted for a high proportion with contents of 2454.94 mg/100 g, 2360.26 mg/100 g, and 1462.11 mg/100 g, respectively. This suggests that the layer is an excellent source of BCAA. In addition, nine non-essential amino acids, including arginine, alanine, aspartic acid, glutamic acid, glycine, histidine, serine, proline, and tyrosine, were detected. Among them, glutamic and aspartic acids had the highest levels with contents of 3624.97 mg/100 g and 3289.47 mg/100 g, respectively. On the other hand, the total amino acid content of dried layer from different regions of Republic of Korea was reported to range from 156 to 310.9 mg/g [32]. \begin{table}
\begin{tabular}{c c c} \hline
**Amino Acid (A.A)** & **Dried-Layer** & **Surimi** \\ \hline Lysine ** & 1823.70 \(\pm\) 6.07 (1961.90) 1 & 1861.14 \(\pm\) 8.08 (7602.69) 1 \\ Threonine ** & 1842.22 \(\pm\) 27.13 (1982.59) & 905.88 \(\pm\) 7.21 (3700.49) \\ Leucine ** & 2454.94 \(\pm\) 58.4 (2641.99) & 1538.11 \(\pm\) 12.03 (6283.13) \\ Isoleucine ** & 1462.11 \(\pm\) 11.00 (1573.51) & 1003.62 \(\pm\) 2.83 (4099.75) \\ Valine ** & 2360.26 \(\pm\) 17.18 (2540.10) & 1011.97 \(\pm\) 13.47 (4133.86) \\ Phenylalanine ** & 1350.15 \(\pm\) 30.62 (1453.02) & 768.59 \(\pm\) 25.30 (3139.67) \\ Tyrosine *** & 1074.80 \(\pm\) 24.45 (1156.69) & 608.08 \(\pm\) 7.54 (2483.99) \\ Argin *** & 2322.33 \(\pm\) 24.20 (2499.28) & 1359.74 \(\pm\) 15.32 (5554.49) \\ Histidine *** & 1025.99 \(\pm\) 12.81 (1104.65) & 676.79 \(\pm\) 5.27 (2764.67) \\ Glutamic acid *** & 3624.97 \(\pm\) 53.47 (3901.17) & 3199.26 \(\pm\) 28.13 (13,068.87) \\ Glycine *** & 2154.22 \(\pm\) 28.42 (2218.36) & 732.31 \(\pm\) 4.91 (2991.46) \\ Serine *** & 1533.69 \(\pm\) 34.12 (1650.55) & 715.12 \(\pm\) 4.82 (2921.24) \\ Proline *** & 1767.27 \(\pm\) 30.70 (1901.93) & 723.67 \(\pm\) 33.25 (2956.17) \\ Alanine *** & 3533.89 \(\pm\) 44.27 (3803.15) & 1078.17 \(\pm\) 7.84 (4404.29) \\ Aspartic acid *** & 3289.47 \(\pm\) 48.54 (3540.10) & 2215.62 \(\pm\) 19.12 (9050.74) \\ \hline Essential A.A & 11,293.38 (12,153.47) & 7089.31 (28,959.60) \\ Non-essential A.A & 20,316.64 (21,864.66) & 11,308.76 (46,195.92) \\ Total & 31,610.02 \(\pm\) 251.33 (34,018.53) & 18,398.07 \(\pm\) 131.29 (75,155.51) \\ \hline
**Free amino acid (E.A.A)** & **Dried-layer** \\ \hline L-Lysine 4 & 16.50 \(\pm\) 1.61 (17.76) \\ L-Threonine 4 & 8.61 \(\pm\) 0.54 (9.27) \\ L-Leucine 5 & 11.53 \(\pm\) 0.62 (12.41) \\ L-Isoleucine 5,* & 8.52 \(\pm\) 0.48 (9.17) \\ L-Valine 5,* & 14.44 \(\pm\) 0.88 (15.54) \\ L-Methionine 5 & tr. 2 \\ L-Phenylalanine 5 & 6.92 \(\pm\) 0.76 (7.48) \\ L-Tryptophan & tr. \\ L-Cystine & tr. \\ L-Tyrosine & 6.28 \(\pm\) 0.15 (6.76) \\ L-Arginine & tr. \\ L-Histidine 5 & tr. \\ L-Glutamic acid 3 & 1110.22 \(\pm\) 2.97 (1194.81) \\ L-Aspartic acid 3 & 125.73 \(\pm\) 4.65 (135.31) \\ L-\(\alpha\)-Aminoadipic acid & tr. \\ L-\(\alpha\)-Aminoobutyric acid & tr. \\ \(\beta\)- Aminobutyric acid & tr. \\ \(\gamma\)-Annothyric acid & 12.82 \(\pm\) 0.57 (13.80) \\ L-Carosine & tr. \\ L-Citrulline & 23.36 \(\pm\) 2.62 (25.14) \\
1-Methyl-L-histidine & tr. \\ \hline \end{tabular}
\end{table} 
Table 2: Amino acid compositions of dried layer and surimi and free amino acid composition of dried layer as major components, (mg/100 g).