# Chemical Composition of Volatile and Extractive Components of Canary (Tenerife) Propolis
## 2 Results  
### Composition of Volatile Components of Propolis  
Determination of the volatile organic components (VOCs) of propolisis was carried out using the method of solid phase microextraction combined with gas chromatography with mass spectrometric detection (HS-SPME/GC-MS). Chromatograms recorded using this method contained peaks of 162 C\({}_{1}\)-C\({}_{20}\) compounds belonging to different classes of organic substances. Table 1 shows the composition of VOCs, grouped by class of organic compounds, as well as the substances included in each group in order of increasing retention index. The largest contribution to the total ion current (TIC) of the chromatograms was made by the group of terpenoids, numbering 72 compounds. The monoterenoid subgroup, formed by 34 individual components (11 of them were found in all three samples), accounted for 27-56% of the TIC. However, 12 monoterpenoids were recorded in only one of the three samples. An even greater difference was observed in the subgroup of sesquiterpenoids: 27 out of a total of 36 were found in only one of the samples. \begin{table}
\begin{tabular}{l c c c c c} \hline \hline  & & & & \multicolumn{3}{c}{**Sample**} \\ \cline{4-6}
**Monoterpene/Monoterpenoids, Including:** & \multirow{2}{*}{_RIExp_} & \multirow{2}{*}{_RI\({}^{Li}\)_} & **Pr-1** & **Pr-2** & **Pr-3** \\ \cline{3-6}  & & & **27.07** & **55.84** & **38.10** \\ \hline tricyclene & 919 & 921 & 0.19 & 0.16 & 0.21 \\ \hline \(\alpha\)-thujene & 925 & 926 & 1.35 & N.d. * & 2.58 \\ \hline \(\alpha\)-pinene & 936 & 936 & 6.43 & 10.07 & 18.68 \\ \hline camphene & 945 & 946 & 0.59 & 1.03 & 0.28 \\ \hline dehydrosabinene & 956 & 957 & N.d. & 1.49 & N.d. \\ \hline sabinene & 970 & 973 & 0.68 & N.d. & 5.55 \\ \hline \(\beta\)-pinene & 975 & 975 & 0.42 & 2.43 & 1.06 \\ myrcene & 990 & 991 & 0.36 & 1.54 & 0.51 \\ \hline \(3\)-carene & 1010 & 1011 & 0.26 & trace ** & 1.76 \\ \hline \(\alpha\)-terpinene & 1016 & 1017 & 0.18 & N.d. & 0.42 \\ \hline limonene & 1028 & 1028 & 13.36 & 4.77 & 1.49 \\ \hline _cis_-\(\beta\)-ocimene & 1044 & 1042 & N.d. & 0.56 & N.d. \\ \hline _trans_-\(\beta\)-ocimene & 1050 & 1048 & N.d. & 0.21 & N.d. \\ \hline \(\gamma\)-terpinene & 1056 & 1057 & 0.30 & N.d. & 0.86 \\ \hline dihydromycenol & 1071 & 1073 & 0.35 & N.d. & N.d. \\ \hline terpinolene & 1086 & 1088 & N.d. & N.d. & 0.57 \\ \hline _trans_-sabinene hydrate & 1096 & 1097 & N.d. & N.d. & 0.19 \\ \hline \(\alpha\)-campholenal & 1124 & 1226 & N.d. & 2.18 & 0.47 \\ \hline _trans_-pinocarveol & 1135 & 1140 & 0.54 & 1.97 & 0.35 \\ \hline _trans_-verbenol & 1142 & 1142 & 0.51 & 0.57 & 0.49 \\ \hline pinocarvone & 1161 & 1164 & N.d. & 1.22 & N.d. \\ \hline borneol & 1165 & 1168 & 0.36 & 1.01 & N.d. \\ \hline isopinocamphone & 1174 & 1175 & N.d. & 0.29 & N.d. \\ \hline \(4\)-terpineol & 1178 & 1178 & 0.11 & 0.84 & 1.16 \\ \hline \(\alpha\)-terpineol & 1189 & 1191 & 0.12 & 7.82 & 0.23 \\ \hline \(\alpha\)-thujenal & 1195 & 1190 & N.d. & 0.38 & N.d. \\ \hline myrtenol & 1198 & 1196 & N.d. & 1.26 & N.d. \\ \hline verbenone & 1209 & 1212 & N.d. & 2.20 & N.d. \\ \hline _trans_-carveol & 1220 & 1218 & N.d. & 2.40 & N.d. \\ \hline _cis_-carveol & 1230 & 1229 & N.d. & 0.59 & N.d. \\ \hline carvone & 1243 & 1245 & N.d. & 0.92 & N.d. \\ \hline carvotanacetone & 1246 & 1249 & N.d. & 0.47 & N.d. \\ \hline bornyl acetate & 1285 & 1287 & N.d. & 3.36 & 0.30 \\ \hline piperitone & 1340 & 1340 & N.d. & 0.20 & - \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Relative group composition (% TIC) of volatile compounds in Canary (Tenerife) propolisols.