# Halophyte _Artemisia caerulescens_ L.: Metabolites from In Vitro Shoots and Wild Plants
## 2 Results and Discussion
### Phytochemical Investigation
#### 2.3.1 Headspace Analysis
** & \multirow{2}{*}{**2**}} & \multirow{2}{*}{**Class**} & \multicolumn{4}{c}{**Relative Abundance \(\pm\) Standar Deviation (\%)**} \\ \cline{5-8}  & & & & & & **Towing** & **Ripe** & **In Vitro** \\  & & & & & **Infloresences** & **Infloresences** & **Shoots** \\ \hline ethyl 2-methylbutyrateate & 850 & 850 & nt & -3 & \(0.1\pm 0.00\) & - & - \\ (_E_)-salveen & 867 & 867 & nt & - & \(0.4\pm 0.15\) & - \\ ethyl isovalerate & 859 & 858 & nt & - & \(1.9\pm 0.22\) & - & - \\ tricyclore & 922 & 927 & mh & - & \(0.3\pm 0.00\) & - & - \\ \(\alpha\)-thujene & 926 & 1102 & mh & \(0.1\pm 0.07\) & \(0.6\pm 0.11\) & \(0.2\pm 0.06\) & - \\ \(\alpha\)-pinene & 933 & 939 & mh & \(0.9\pm 0.18\) & \(3.2\pm 0.05\) & \(0.3\pm 0.09\) & - \\ camphene & 948 & 954 & mh & \(0.6\pm 0.09\) & \(0.8\pm 0.04\) & - \\ sabinene & 973 & 975 & mh & \(5.2\pm 0.82\) & \(16.1\pm 1.06\) & \(6.5\pm 2.04\) & - \\ \(\beta\)-pinene & 977 & 979 & mh & \(0.1\pm 0.02\) & \(0.6\pm 0.03\) & \(0.2\pm 0.08\) & - \\ m-ryceen & 991 & 991 & mh & \(0.2\pm 0.06\) & \(0.3\pm 0.02\) & - & 0.7 \(\pm 0.38\) \\ \(2\)-methylbutyl isobutyrate & 1016 & 1017 & nt & \(0.3\pm 0.03\) & \(0.2\pm 0.02\) & - \\ \(\alpha\)-terpinene & 1027 & 1017 & mh & \(0.1\pm 0.02\) & \(0.2\pm 0.01\) & \(0.1\pm 0.01\) & - \\ p-cymene & 1025 & 1025 & mh & \(1.4\pm 0.16\) & \(0.6\pm 0.03\) & \(1.7\pm 0.25\) & - \\ limnene & 1029 & 1029 & mh & \(0.3\pm 0.05\) & \(0.9\pm 0.17\) & \(0.2\pm 0.05\) & - \\ \(1\)-Scinede & 1031 & 1031 & om & \(1.2\pm 0.04\) & \(1.2\pm 0.06\) & \(1.2\pm 0.14\) & \(0.8\pm 0.22\) \\ \(\gamma\)-terpinene & 1058 & 1060 & mh & \(0.3\pm 0.02\) & \(0.3\pm 0.00\) & \(0.6\pm 0.10\) & - \\ \(\alpha\)-thujene & 1107 & 1102 & om & \(67.9\pm 1.56\) & \(45.4\pm 0.04\) & \(78.1\pm 2.56\) & \(83.2\pm 1.41\) \\ \(\beta\)-thujene & 1117 & 1114 & om & \(8.0\pm 0.64\) & \(112.1\pm 1.80\) & \(9.7\pm 0.28\) & \(7.6\pm 0.16\) \\ chrysanthenone & 1126 & 1128 & om & \(2.1\pm 0.43\) & \(2.6\pm 0.54\) & & - \\ _trans_-pniconxved & 1139 & 1139 & om & \(0.2\pm 0.03\) & - & - & - \\ _(_Z_)-zigetnene & 1231 & 1229 & om & \(0.6\pm 0.02\) & - & - & - \\ _(E_)-zigetnene & 1240 & 1238 & om & \(0.3\pm 0.02\) & - & - & - \\ iso-pipertiene & 1271 & 1272 \({}^{\star}\) & om & \(0.7\pm 0.02\) & - & - & - \\ perilla aldehyde & 1273 & 1272 & om & - & \(0.5\pm 0.30\) & - & - \\ _(E_,_Z_)-4,d-decdenal & 1316 & 1317 & nt & \(0.2\pm 0.03\) & - & - & - \\ cycloosterive & 1367 & 1371 & sh & - & - & \(1.2\pm 0.26\) \\ \(\alpha\)-copene & 1376 & 1377 & sh & \(0.4\pm 0.06\) & - & - & \(1.7\pm 0.10\) \\ p-elemene & 1392 & 1391 & sh & - & - & \(0.6\pm 0.11\) & - \\ \(2\)-ethylidene-6-methyl-3,5-heptateli & 1395 & 1395 & om & \(6.4\pm 1.01\) & - & - & - \\ cyrere & 1399 & 1399 & sh & & & \(2.0\pm 0.68\) \\ _(E_)-\(\beta\)-d-znesene & 1458 & 1457 & sh & & & \(1.3\pm 0.17\) \\ germacene D & 1481 & 1485 & sh & & & \(0.8\pm 0.20\) \\ \(\beta\)-elemene & 1486 & 1490 & sh & \(0.3\pm 0.02\) & - & - & - \\ l-calion & 1641 & 1640 & os & & & \(0.7\pm 0.10\) \\ bewifolin & 1669 & 1675 \({}^{\star}\) & PP & \(0.2\pm 0.03\) & - & - & - \\ \hline Total identified (\%) & & & & & \(97.3\pm 0.06\) & \(100.0\pm 0.01\) & \(100.0\pm 0.01\) & \(100.0\pm 0.01\) \\ \hline \multirow{2}{*}{Chemical Classes} & & & & & Young & \multirow{2}{*}{Infloresences} & \multirow{2}{*}{Infloresences} & \multirow{2}{*}{In Vitro Shoots} \\  & & & Leaves & Infloresences & & & \\  & & & & & (Bosom) & & \\ \hline Monoterpene hydrocarbons (mh) & & & \(9.3\pm 0.95\)\({}^{\rm{B}}\) & \(27.2\pm 0.13\)\({}^{\rm{A}}\) & \(9.8\pm 2.66\)\({}^{\rm{B}}\) & \(0.7\pm 0.38\)\({}^{\rm{C}}\) \\ Oxygenated monoterreprene (om) & & & \(87.0\pm 0.97\)\({}^{\rm{B}}\) & \(70.2\pm 0.34\)\({}^{\rm{C}}\) & \(89.1\pm 2.72\)\({}^{\rm{B}}\) & \(91.6\pm 1.35\)\({}^{\rm{A}}\) \\ Sesquiretenpe hydrocarbons (sh) & & & \(0.6\pm 0.08\)\({}^{\rm{B}}\)were plotted in the right quadrants (PC1 > 0); although the ripe infloresences and the in vitro shoot HSs were located in the bottom quadrant (PC2 < 0), the HS of leaves was plotted on the partitioning line between the upper left and right quadrants, probably due to its higher content of 2-ethylidene-6-methyl-3,5-heptadienal.