## 2 Materials and Methods
### Antioxidant Activity  
DPPH Assay  
Scavenging capacity of electrospun mats towards 2,2-diphenyl-1-picrylhydrazyl free radicals (DPPH) was measured using a slightly modified method originally presented by [PERSON] et al. [22]. For that purpose, methanolic solution of the DPPH reagent (65 \(\upmu\)M) was freshly prepared and adjusted with methanol to reach absorbance of 0.70 (\(\pm\)0.02). DPPH reagent (2.9 mL), methanol (0.1 mL) and electrospun mats (10 mg) were mixed in the 10 mm glass cuvettes and incubated at room temperature for 60 min. Absorbance was further measured at 517 nm in \begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Sample Name**} & **Polymer** & **Active** & **Feed Rate** & **Needle-to-Collector** & \multirow{2}{*}{**Voltage (kV)**} \\  & **Weight (g)** & **Compound** & **(\(\upmu\)L/h)** & **Distance (cm)** & \\ \hline PLA & 1.6 & - & 2000 & 12 & 16 \\ PVP & 0.78 & - & 500 & 10 & 12 \\ PLA-5 SB & 1.614 & 0.08 & 2200 & 13 & 15.5 \\ PLA-10 SB & 1.625 & 0.162 & 2500 & 15 & 15 \\ PVP-5 SB & 0.784 & 0.0392 & 750 & 10 & 13 \\ PVP-10 SB & 0.788 & 0.078 & 1000 & 12 & 14 \\ PLA-5 PSO \({}^{\star}\) & 1.614 & 0.08 & 2200 & 12 & 15 \\ PLA-10 PSO & 1.625 & 0.162 & 2200 & 15 & 14 \\ PVP-5 PSO & 0.784 & 0.0392 & 750 & 12 & 12 \\ PVP-10 PSO & 0.788 & 0.078 & 1000 & 12 & 14 \\ PLA-5 PSOSEP & 1.625 & 0.162 & 2000 & 12 & 13 \\ PVP-5 PSOSEP & 0.788 & 0.078 & 1000 & 10 & 12 \\ PVP-10 EP & 0.788 & 0.078 & 1000 & 10 & 11 \\ \hline \hline \end{tabular}  
* PLA-5 PSO signify the sample which contains polylactide (PLA) and 5 wt% of pomeranate seed oil.  
\end{table} 
Table 1: Process Parameters. triplicates with UV-VIS spectrophotometer (6300 Spectrophotometer, Jenway, UK). DPPH inhibition was expressed according to following equation [23]:  
\[\textit{Inhibition}\ [\%]=\frac{\left(A_{control}-A_{sample}\right)}{A_{control}} \times 100\]  
where \(A_{control}\) and \(A_{sample}\) are the absorbance values of the DPPH solution without and with the presence of the nonwoven samples. The measurements were carried out in triplicate and results were expressed as mean \(\pm\) standard deviation. FRAP Assay  
Reduction capacity of electrospun mats towards Fe\({}^{3+}\) was measured using slightly modified method firstly presented by [PERSON] [24]. The FRAP reagent was freshly prepared from 300 mM acetate buffer (pH = 3.6), 10 mM 2,4,6-tris(2-pyridil)-s triazine (TPZT), 40 mM HCl solution and 20 mM FeCl\({}_{3}\) aqueous solution. Solutions were mixed in the ratio 10:1:1 (v/v/v). Electrospun mats (10 mg), FRAP reagent (2.8 mL) and acetate buffer (0.1 mL) were mixed and incubated in the dark at 37 \({}^{\circ}\)C for 10 min. Absorbance measurements were performed at 593 nm (in triplicates) with UV-VIS spectrophotometer (6300 Spectrophotometer, Jenway, UK). The measurements were carried out in triplicate and results were expressed as reduction percentage of Fe\({}^{3+}\) as mean \(\pm\) standard deviation [%]. ABTS Assay  
The ability of fibers towards scavenging of ABTS free radicals was measured using a modified method previously reported [25]. ABTS stock solution was freshly prepared from a mixture (1:1, v/v) of 2.45 mM potassium persulfate aqueous solution and 7 mM ABTS (2,2'-azino-bis-(-3-ethylbenzothazoline-6-sulfonic acid) diammonium salt) aqueous solution and left in the dark at room temperature for 16 h. A stock solution was diluted using 300 mM acetate buffer (pH = 3.6) to an absorbance of 0.70 (\(\pm\)0.02). Electrospun mats (10 mg), ABTS reagent (2.9 mL) and acetate buffer (0.1 mL) were mixed and stored in the dark at room temperature for 5 h. Absorbance was further measured at 734 nm in triplicates with UV-VIS spectrophotometer VIS spectrophotometer (6300 Spectrophotometer, Jenway, UK). Freshly prepared Trolox aqueous solutions (0-0.8 mM, R2 = 0.987) were used to obtain the calibration curve. The measurements were carried out in triplicate and results were expressed as percentage of inhibition (mean \(\pm\) standard deviation).