## 3 Materials and Methods  
### Standards and Reagents  
Chlorpromazine, clozapine, olanzapine, and quetiapine were purchased from Cerilliant (Round Rock, TX, USA). Desmethylchlorpromazine and 7-hydroxy-chlorpromazine were acquired from TRC Canada (Toronto, ON, Canada). \(N\)-desmethylclozapine was obtained from Sigma-Aldrich (St. Louis, MO, USA). \(N\)-desmethylclozapine was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Norquetiapine was acquired from Biovision (Milpitas, CA, USA). The internal standards chlorpromazine-d3 and quetiapine-d8 were obtained from Cerilliant (Round Rock, TX, USA). Butyl methacrylate (BMA) (99%), ethylenglicl olmateracrylate (EGDMA, 98%), 1-propanol (HPLC grade), vinyltrimethoxysilane (VTMS) 1,4-butendi (99%), and 2,2-azobisisobutylnitrile (AlBN) were  
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Drugs**} & \multicolumn{2}{c}{**Therapeutic Drug Monitoring**} & \multicolumn{4}{c}{**Plasma Concentrations**} \\ \cline{3-6}  & \multicolumn{2}{c}{**Interval (ng mL\({}^{-1}\))**} & **Patient 1** & **Patient 2** & **Patient 3** & **Patient 4** \\ \hline Chlorpromazine & 30–300 & - & - & - & 329 \\ Cloazapine & 350–600 & - & - & 528 & - \\ Olanzapine & 20–80 & 85 & - & - & - \\ Quetiapine & 100–500 & - & 400 & - & - \\ Desmethyl & - & - & - & - & 12 \\ chlorpromazine & - & - & - & - & 40 \\ \(N\)-desmethyl clozapine & - & - & - & 328 & - \\ \(N\)-desmethyl olanzapine & - & 19 & - & - & - \\ Norquetiapine & - & & 61 & - & - \\ \hline \hline \end{tabular}
\end{table}
Table 6: Concentrations of the target antipsychotic and their metabolites in plasma samples from Schizopheneric patients.  
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multirow{2}{*}{**Analytes**} & \multirow{2}{*}{**Matrix**} & \multicolumn{2}{c}{**Sample**} & \multicolumn{2}{c}{**Chromographic**} & \multicolumn{2}{c}{**Analytical**} & \multirow{2}{*}{**LLOQ (ng mL\({}^{-1}\))**} & \multirow{2}{*}{**References**} \\ \cline{1-1} \cline{6-7}  & & **Amount (\#L)** & & & & & \\ \hline Aripipyrazole & & & & & & \\ Olanzapine & & & & & & \\ Paliperidone & & & & & & \\ Ziprisadone & & & & & & \\ chlorpromazine, & & & & & & \\ haloperidol, & & & & & & \\ levorempromazine, & & & & & & \\ olanzapine, & & & & & & 2.9 (olanzapine) & \\ silpiride & & & & & & \\ Haloperidol, & & & & & & \\ olanzapine, & & & & & & \\ chlorpromazine, & & & & & & \\ quetiapine, & & & & & & \\ cloazapine & & & & & & \\ Clozapine, & & & & & & \\ risperidone, and & & & & & & \\ chelolip & & & & & & \\ chlorpromazine, & & & & & & \\ clozapine, & & & & & & \\ quietapine, & & & & & & \\ olanzapine and & & & & & & \\ metabolites & & & & & & \\ \hline \hline \end{tabular}
\end{table}
Table 5: Comparison of the in-tube SPME-UHPLC-MS/MS method with other counterparts to determine antipsychotics and their metabolites in biological samples.  
purchased from Sigma-Aldrich (St. Louis, MO, USA). Fused silica capillary (530 \(\upmu\)m i.d. \(\times\) 10 cm) was acquired from NST (Sao Paulo, Brazil). Acetonitrile, methanol (HPLC grade), ammonium acetate, and formic acid were supplied by JTBaker (Phillisburg, NJ, USA). Hydrochloric acid and sodium hydroxide were purchased from Sigma-Aldrich (St. Louis, MO, USA). Aqueous solutions were prepared with ultrapure water from a Milli-Q, Millipore system (18.2 M\(\Omega\) cm) (Sao Paulo, SP, Brazil).