###### Abstract  
Exercise is an important component in the treatment and improvement of function in populations with or at risk of lower limb injury. Cycling is the most common exercise modality used by these populations. However, reduced lower limb joint excursion and/or range of motion (ROM) during cycling might limit the optimization of functional improvements. Increased hip flexion gait (HFgait) is a new exercise modality that might result in larger lower limb joint excursions compared to cycling. The purpose of this study was to compare lower limb kinematics between HFgait and cycling. Twelve healthy individuals participated in the study. Each participant performed cycling and HFgait. Hip, knee, and ankle kinematics in the sagittal, frontal, and transverse planes were analyzed with and without phase offset reduction (POR). Discrete and continuous analyses were performed. Discrete analysis indicated differences for at least one of the variables analyzed (maximum, minimum, and ROM) for the hip (\(p\leq 0.041\)), knee (\(p\leq 0.008\)), and ankle (\(p\leq 0.040\)) across all planes. For the continuous analysis, differences between HFgait and cycling kinematics were observed during the cycles for the hip, knee, and ankle sagittal (hip: original: 85%; with POR: 77%; knee: original: 93%; with POR: 76%; ankle: original: 14%; with POR: 14%), frontal (hip: original: 93%; with POR: 98%; knee: original: 41%; with POR: 12%; ankle: original: 4%; with POR: 5%), and transverse (hip: original: 66%; with POR: 0%; knee: original: 14%; with POR: 0%; ankle: original: 13%; with POR: 0%) planes. HFgait resulted in larger hip (+60.2\({}^{\circ}\)) and knee (+38.2\({}^{\circ}\)) sagittal plane ROM while maintaining the hip in a more neutral position in the frontal plane compared with cycling. These findings can support the development of rehabilitation strategies with the goal of improving function and joint range of motion while also receiving the health benefits of exercise. regular exercise of moderate intensity for the prevention and complementary treatment of several diseases. However, participation in physical activity and exercise can be largely affected by pain or risk of injury. Clinical populations that experience pain and have functional deficits have reduced availability of exercise modalities and intensities, which can limit the optimization of the health and functional benefits received from exercising [1]. While running and cycling are two commonly used, convenient, and easy-to-perform exercise modalities, populations that experience lower-extremity pain typically prefer cycling. This is often the case in individuals with osteoarthritis. For example, [PERSON] et al. [4] have reported good adherence and tolerability in a feasibility study of an exercise program in individuals with knee osteoarthritis when individuals were allowed to self-select the exercise modality. In this study, only 3 out of 24 participants (14%) chose treadmill running to cycling. This indicates a clear preference towards cycling when running is the alternative in individuals with osteoarthritis. This may be related to the weight bearing component associated with running that will result in increased lower joint loadings and subsequent increase in pain [5; 6]. However, interventions using cycling as the exercise mode, despite allowing for individuals with osteoarthritis to exercise at high intensities, have reported a lack of effectiveness for improving physical function, daily activity, quality of life, and mobility in individuals with knee osteoarthritis [7].