## 3 Results and Discussion
### Profile of Phenolic Compounds  
Phenolic compounds are a class of natural secondary metabolites highly present in a different part of plants that has gained a lot of scientific and medicinal attention because of the wide variety of beneficial properties such as antioxidant and anti-inflammatory qualities that may be beneficial in the prevention and/or treatment of cardiovascular disease, neurological diseases, cancer, and obesity [40]. As can be seen in Table 3, the present study identified 13 out of 22 analyzed phenolic compounds in _Laminum album_ flower extract obtained by SC-CO\({}_{2}\), which comprised flavones, phenylethanolamine isomers and flavanone. The mean phenolic compounds concentration varied from 0.09 (_trans_-cinnamic acid, at 40 \({}^{\circ}\)C) to 22.68 ng/uL (chrysin, at 50 \({}^{\circ}\)C). Apart from chrysin, high levels of concentration for extracts obtained at 50 \({}^{\circ}\)C have been reported for myricetin, pinostrobin, and _trans_-3-hydroxycinnamic acid. The concentration of the most analyzed bioactive compounds was statistically higher (\(p\) = 0.05) for the extract obtained at the temperature of 50 \({}^{\circ}\)C, especially in comparison with the extraction conditions at the lower temperature of 40 \({}^{\circ}\)C. On the other hand, for the extraction carried out at the highest temperature 60 \({}^{\circ}\)C, the level of most bioactive compounds was significantly lower compared to the extraction at 50 \({}^{\circ}\)C, except for tritin, quercetin and trans-cinnamic acid. The obtained results are consistent with the highest antioxidant activity for the extract obtained at 50 \({}^{\circ}\)C, confirmed by four methods (Tables 1 and 2). In the literature, very little information is available concerning the phenolic compounds of the studied plant (_Laminum album_ flowers) and their application. However, in the previous studies, some flavonoids in forms of glycosides (rutin, isoquercitrin, tyliroside) and aglycones (quercetin) and phenolic acids (protocatechuic, chlorogenic, vanillic, caffeic, coumaric, and ferulic acids) have been found in methanol and ethyl acetate extracts of _L. album_ by using HPTLC combined with densitometry and HPLC method [8]. For the first time, [PERSON] et al. (2012) [5] identified derivatives of unusual flavone isoscutellarein in purified ethanol extract of flowers, leaves and stems from _L. album_, and were found to represent approximately one third of the total phenolics quantified and also phenylethanolamine glycosides verbascoside and iso-verbascoside have been reported [5]. To the best of our knowledge, most of those phenolic compounds were identified in _Laminum album_ for the first time in the present study. Chrysin (5,7-dihydroxyflavone) is a major compound detected in _Laminum album_ extract in this study. It is a flavone, naturally present in flowers of various plants, honey and some mushrooms [41], it has a lot of health importance for humans. Chrysin was reported to have enormous biological activities such as antibacterial, antidiabetic, anticancer, anti-inflammatory, and anti-aging [42; 43]. Furthermore, a recent clinical trial found that using chrysin can result in body weight loss and a lower postprandial blood-glucose level [41]. In addition to its medicinal use as