###### Abstract  
Natural phytontutrients in foods, including anthocyanins, can play an important role in human health. Anthocyanins have been reported to cause many various useful effects, such as reducing cancer cell proliferation, regulating blood pressure, preventing tumor formation, and preventing diabetes. In this study, we aimed to reveal the qualitative anthocyanin content, antiproliferative and antimicrobial effects of different extracts derived from _Vaccinium myrtillus_, _Vaccinium corymosum_, _Sambucus nigra_ and _Aronia melanocarpa_. The anthocyanin content of the plants mentioned in the study was characterized after the freeze-drying process. MTT assay was chosen to determine the antiproliferative effect of extracts on colorectal cancer cells. Antimicrobial effects of extracts were studied on typical and clinical strains of five different bacteria. As a result, it was determined that the anthocyanin content in the extracts obtained by the freeze-drying method was acceptable, and some extracts were found to have a strong antiproliferative effect on colorectal cancer cells and have an antibacterial effect on typical and clinical strains. Conclusionally, it has been shown that anthocyanins, which are reported to have many beneficial effects, are very promising when prepared with a relatively new method, and this study is a pioneer study for possible future pharmacological and in vivo studies. annocyanins; antimicrobial; berries; cytotoxic; extraction; lyophilization +
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The difference between the individual aglycon types depends on a number of linked hydroxylated groups and their methylation level. The most common anthocyanins are cyanidine, delphinidine, peonidine, malvidine, pelargonidine and petunidine. At the same time, fruits and phytontutrients have an important role in the prevention and treatment of various diseases. The beneficial effects of anthocyanins on human health have been known since the 16 th century. For example, the use of blackberries to treat eye and mouth infections is very old [2]. Moreover, natural anthocyanins have been extensively studied for their medicinal potential. Anthocyanins have protective effects on cardiovascular diseases as well as anti-inflammatory, anticancer, antimicrobial, anti-obesity and antidiabetic effects [3]. The antioxidant properties of anthocyanin extracts from fruits of various plant species have been the subject of several research works [4; 5; 6]. The results indicated that all berry samples had a good antioxidant activity that depends on several factors, such as plants genotype, soil-climatic properties, various anthocyanin isolation and test (DPPH\({}^{+}\), ABTS\({}^{+}\), TEAC) methods. Based on total phenolic compounds concentration (from 3567 mg/100 g to 1050 mg/100 g) and antioxidant capacities (from 84.5% to 62.8% of radical scavenging capacity) the investigated berry species may be ranked as follows: _Aronia melanocarpa_ Wild. > _Sambucus nigra_ L > _Vaccinium myrtillus_ L. > _Vaccinium corymbosum_ L. The IC\({}_{50}\) values and the Trolox equivalent antioxidant capacity (TEAC) values were the highest for _Aronia melanocarpa_ ethanolic extract, while the acetone sample prove to be nine time less active. Cancer is an insidious disease with a high mortality rate in the human population. Cancer cells differ from normal cells in terms of morphology and function. Anthocyanins act on cancer cells due to these differences. The most important feature of cancer cells is the loss of control over cell division and subsequent continuous proliferation. Anthocyanin-rich extracts and pure anthocyanins have been reported to inhibit cell proliferation by blocking various stages of the cell cycle in cancer cells [7; 8]. Previous studies have reported anti-proliferative effects of the anthocyanin-rich extracts on different cancer cell lines such as HT-29, MCF-7, HEp-2, MKN-45, HL-60 and A549 [9; 10].