## 5 Microarray Results
### Gene Ontology and Gene Set Enrichment Analysis
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Enrichment FDR** & **nGenes** & **Pathway** & **Fold Enrichment** & **Pathway** & **Genes** \\ \hline
0.001010066 & 5 & 60 & 13.50925926 & Type I diabetes mellitus & H2-Aa H2-Eb1 H2-K1 H2-Q6 H2-Ab1 \\ \hline
0.014935096 & 3 & 37 & 13.14414414 & Thyroid cancer & Gadd45b Gadd45g Myc \\ \hline
0.001739711 & 5 & 69 & 11.74718196 & Autoimmune thyroid disease & H2-Aa H2-Eb1 H2-K1 H2-Q6 H2-Ab1 \\ \hline
0.018879359 & 3 & 42 & 11.57936508 & Intestinal immune network for IgA production & H2-Aa H2-Eb1 H2-Ab1 \\ \hline
0.007502182 & 4 & 62 & 10.45878136 & Longevity-regulating pathway & Hspa8 Irs2 Irs1 Hspa1a \\ \hline
0.000350761 & 7 & 109 & 10.4108053 & Toxoplasmaosis & Hspa8 H2-Aa H2-Eb1 H2-Ab1 \\ \hline
0.00250099 & 5 & 78 & 10.39173789 & Viral myocarditis & H2-Aa H2-Eb1 H2-K1 H2-Q6 H2-Ab1 \\ \hline
0.003489764 & 5 & 86 & 9.425064599 & Rheumatoid arthritis & Fos Ccl5 H2-Aa H2-Eb1 H2-Ab1 \\ \hline
0.009996389 & 4 & 69 & 9.397745572 & Leishmaniasis & Fos H2-Aa H2-Eb1 H2-Ab1 \\ \hline
0.003489764 & 5 & 87 & 9.316730524 & Th1 and Th2 cell differentiation & Fos Cd4 H2-Aa H2-Eb1 H2-Ab1 \\ \hline
0.010543292 & 4 & 71 & 9.133020344 & Drug metabolism & Mgst1 Aldh3b3 & Fmo2 Aox1 \\ \hline
0.00656708 & 5 & 104 & 7.793803419 & Th17 cell differentiation & Fos Cd4 H2-Aa H2-Eb1 H2-Ab1 \\ \hline
0.018879359 & 4 & 88 & 7.368686869 & Colorectal cancer & Gadd45b Fos Gadd45g Myc \\ \hline
0.019277334 & 4 & 91 & 7.125763126 & IL-17 signaling pathway & Fos Fos Lcn2 Cxcl10 \\ \hline
0.019315597 & 4 & 92 & 7.048309179 & Hematopoietic cell lineage & Cd4 H2-Aa H2-Eb1 H2-Ab1 \\ \hline
0.000254132 & 10 & 240 & 6.75462963 & Human T-cell leukemia virus 1 infection & H2-Aa Egr1 Zfp36 H2-Eb1 H2-K1 H2-Q6 H2-Ab1 \\ \hline
0.001990294 & 7 & 169 & 6.714661407 & Influenza A & Drajb1 II33 Cxcl10 Ccl5 H2-Aa H2-Eb1 H2-Ab1 \\ \hline
0.000476756 & 9 & 220 & 6.631818182 & Epstein–Barr virus infection & Gadd45b Gadd45g Myc Cxcl10 H2-Aa H2-Eb1 H2-K1 H2-Q6 H2-Ab1 \\ \hline
0.013590203 & 5 & 131 & 6.187446989 & FoxO signaling pathway & Gadd45b Gadd45g Plik2 Irs2 Irs1 \\ \hline \hline \end{tabular}
\end{table} 
Table 7: Cont. Gel Shift and Western Blot Analysis Shows PHD1\({}^{-/-}\) MI Mice Had Significantly Increased HIF 1\(\alpha\) Expression, HSPA12B, and IRS2 Compared to WTMI Mice  
Gel shift analysis for HIF1\(\alpha\) showed no significant changes in the sham groups. However, the PHD1\({}^{-/-}\) mice showed increased HIF-1\(\alpha\) expression after MI compared to the WTMI group (Figure 8A). The Western blot analysis for the heat shock protein HSPA12B is shown in Figure 8B. The expression level of the protein HSPA12B was increased in the PHD1\({}^{-/-}\)Sham group as compared to the WT sham group (\(p\) = 0.0283, n = 3). MI group comparisons also showed significantly increased HSPA12B (small-molecular-weight heat shock protein) expression in PHD1\({}^{-/-}\) mice compared to WT (\(p\) = 0.0433. n = 3) (Figure 8B). We also selected one of the genes, IRS2, which was increased in Affymetrix analysis and validated by Western blot analysis. We performed immunoprecipitation and immunoblotting of IRS2 protein using IRS2 antibody. Our results showed no significant difference between the sham groups. However, we found significantly increased expression of IRS2 protein in the PHD1\({}^{-/-}\)MI group compared to the WTMI group (\(p\) = 0.0252, n = 3) (Figure 8C).