## 2 Materials and Method
### Determination of Antibacterial Activity  
#### 2.3.1 Strains and Materials  
Five nosocomial bacterial strains were tested: three Gram strain negative, which belong to the Order (Ord.) of Enterobacterales Escherichia coli (_E. coli_), Proteus mirabilis (_P. mirabilis_), Klebsiella pneumoniae (_K. pneumoniae_), and two Gram stain positive: one belonging to Ord. LactobacillusEnterococcus faecalis (_E. faecalis_) and one belonging to Ord. Bacillales--Staphylococcus aureus (_S. aureus_).  
\begin{table}
\begin{tabular}{c c c c c c c c c c c} \hline \hline
**Sample** & **Gallic Acid** & **Epictachin** & **Caffeic Acid** & **B-Resorcylic Acid** & **p-Counaric Acid** & **P-Counaric Acid** & **Resoramic Acid** & **Resveratrol** & **Quercetin** \\ \hline WF & nd\({}^{*}\) & 6.50 \(\pm\) 0.05\({}^{*}\) & nd\({}^{*}\) & 1.20 \(\pm\) 0.01\({}^{*}\) & nd\({}^{*}\) & nd\({}^{*}\) & 3.50 \(\pm\) 0.02\({}^{*}\) & 3.10 \(\pm\) 0.01\({}^{*}\) & 0.95 \(\pm\) 0.02\({}^{*}\) \\ PS & nd\({}^{*}\) & nd\({}^{*}\) & nd\({}^{*}\) & nd\({}^{*}\) & 14.50 \(\pm\) 0.85\({}^{*}\) & nd\({}^{*}\) & 2.16 \(\pm\) 0.01 & nd\({}^{*}\) & 14.26 \(\pm\) 0.85 & nd\({}^{*}\) & 4.07 \(\pm\) 2.1\({}^{*}\) \\ RS & nd\({}^{*}\) & 919.94 \(\pm\) 2.56\({}^{*}\) & nd\({}^{*}\) & nd\({}^{*}\) & 13.01 \(\pm\) 0.2\({}^{*}\) & 62.76 \(\pm\) 2.2\({}^{*}\) & 62.11 \(\pm\) 0.06\({}^{*}\) & 229.95 \(\pm\) 1.10\({}^{*}\) & 14.98 \(\pm\) 1.10\({}^{*}\) \\ BS & nd\({}^{*}\) & 135.63 \(\pm\) 1.54\({}^{*}\) & nd\({}^{*}\) & 25.43 \(\pm\) 1.32\({}^{*}\) & 8.06 \(\pm\) 0.3\({}^{*}\) & nd\({}^{*}\) & 7.11 \(\pm\) 0.02\({}^{*}\) & 19.83 \(\pm\) 1.12\({}^{*}\) & 5.01 \(\pm\) 1.2\({}^{*}\) \\ LS & nd\({}^{*}\) & 86.50 \(\pm\) 3.20\({}^{*}\) & nd\({}^{*}\) & 92.23 \(\pm\) 2.22\({}^{*}\) & 6.83 \(\pm\) 0.60\({}^{*}\) & 30.76 \(\pm\) 4.1\({}^{*}\) & 35.20 \(\pm\) 1.8\({}^{*}\) & 76.35 \(\pm\) 4.20\({}^{*}\) & 75.22 \(\pm\) 0.5\({}^{*}\) \\ \hline  & & & & & & & & & \\ \end{tabular}
\end{table}
Table 1: The individual polyphenol content expressed as mg/kg, detected as 280 nm.  
Isolation and confirmation of coliforms were carried out on different selective culture media--MacConkey agar (Mikrobiologie-Labor-Technik RO, Arad, Romania), Chromatic Detection Agar (Mikrobiologie-Labor-Technik RO) and Eosine Methylene Blue (EMB--Levine) as well as on confirmation media--Triple Sugar Iron (TSI) Agar, Simmons Citrate Agar and Mobility Indole Urease (MIU) Agar (Mikrobiologie-Labor-Technik RO), specifically for _E. coli, P. mirabilis_, and _K. pneumoniae_.  
The isolation and confirmation of the _E. faecalis_ germ were carried out on the selective culture media Chromatic Detection Agar (Mikrobiologie-Labor-Technik RO) and Bile Aesculin Azide Agar (Mikrobiologie-Labor-Technik RO), while for the isolation and confirmation of the _S. aureus_ germ culture media, Chapman agar (Mannitol Salt agar) and Columbia agar with 5% ram red blood cells (Mikrobiologie-Labor-Technik RO) were used, respectively.  
The cultivation was carried out in Petri plates with a diameter of 100 mm by streaking with the help of sterile microbiological loops in aseptic conditions, and the resulting plates were incubated for 24 h between 40 and 42 \({}^{\circ}\)C for coliforms and between 35 and 37 \({}^{\circ}\)C, for faecalis enterococci and for Staphylococcus [22].