## 5 Conclusions  
The whole genome of _Sphingomonas morindae_ sp. NBD5 consists of two circular chromosomes of 4,239,716 bp with 3882 protein coding genes including 59 protein-coding genes related to MP biosynthesis, of which four genes (_ackA_, _pgm_, _gpm1_ and _pckA_) are unique. These genes, _pckG_, _porB_, _meh_, and _fldA_, are unique in _Sphingoryxis_ sp. USTB-05. Lutein and zeaxanthin synthesis metabolic pathways and synthetic genes are discovered in _Sphingomonas morindae_ sp. NBD5 and _Sphingopyxis_ sp. USTB-05. Three new enzymes are found in whole metabolic pathway, they are GGPP synthase, lycopene epsilon-cyclase, carotenoid epsilon hydroxylase. This study expands the understanding of the pathway for complete biosynthesis of MP by _Sphingomonas morindae_ sp. NBD5 and _Sphingopyxis_ sp. USTB-05.  
**Supplementary Materials:** The following supporting information can be downloaded at: [[https://www.mdpi.com/article/10.3390/microorganisms11020266/s1](https://www.mdpi.com/article/10.3390/microorganisms11020266/s1)]([https://www.mdpi.com/article/10.3390/microorganisms11020266/s1](https://www.mdpi.com/article/10.3390/microorganisms11020266/s1)), Figure S1: Sequence distribution of data lengths of nanopore sequencing; Figure S2: Comparison of genes related to lutein synthesis in the genomes of _Sphingomonas morindae_ sp. NBD5 and _Sphingopyxis_ sp. USTB-05; Figure S3: Comparison of genes related to glycolysis and gluconeogenesis pathway in the genomes of _Sphingomonas morindae_ sp. NBD5 and _Sphingopyxis_ sp. USTB-05; Figure S4: Comparison of genes related to terpenoid backbone biosynthesis in the genomes of _Sphingomonas morindae_ sp. NBD5 and _Sphingopyxis_ sp. USTB-05; Figure S5: Comparison of genes related to carotenoid biosynthesis in the genomes of _Sphingomonas morindae_ sp. NBD5 and _Sphingopyxis_ sp. USTB-05; Figure S6: (a) Peaks and scanning profiles of standard lutein; (b) Peaks and scanning profiles of the extract of _S. morindae_ NBD5 on LC-Q-TOF/MS [15]; Figure S7: (a) First-order mass spectrometry of standard lutein on LC-Q-TOF/MS; (b) First-order mass spectrometry of the extract of _S. morindae_ NBD5 on LC-Q-TOF/MS [15]; Figure S8: (a) Second-order mass spectrometry of standard lutein on LC-Q-TOF/MS; (b) Second-order mass spectrometry of the extract of _S. morindae_ NBD5 on LC-Q-TOF/MS [15]; Table S1: Gene Ontology (GO) classifications of genes; Table S2: Clusters of Orthologous Group (COG) classifications of genes.  
**Author Contributions:** Conceptualization, H.Y. and Y.L.; data curation, C.L.; investigation, C.L.; methodology, C.Y.; project administration, Q.X.; resources, Y.L.; software, C.L.; supervision, C.Y.; visualization, C.L.; writing--original draft, C.L.; writing--review and editing, Z.Z., Q.X., H.Z., X.L., H.Y. and Y.L. All authors have read and agreed to the published version of the manuscript.  
**Funding:** This research was funded by the National Natural Science Foundation of China (21677011) and the Fundamental Research Funds for the Central Universities (FRF-TP-20-044A2; FRF-MP-20-39).  
**Institutional Review Board Statement:** Not applicable.  
**Informed Consent Statement:** Not applicable.  
**Data Availability Statement:** The data presented in this study are available in article [[https://doi.org/10.3390/toxins14050333](https://doi.org/10.3390/toxins14050333)]([https://doi.org/10.3390/toxins14050333](https://doi.org/10.3390/toxins14050333)).  
**Conflicts of Interest:** The authors declare no conflict of interest.