## 11 Nanotechnology for Sustainable Environment, Agriculture and Food Safety
### Advanced Molecular Screening of Bacterial Contaminants Found in Local Wastewater [PERSON]  
Treated wastewater from reclaimed facilities (WWTP) has become a reusable source for a variety of applications, such as agricultural irrigation. However, it is also a potential reservoir of clinically relevant multidrug resistant (MDR) pathogens, including ESKAPE (Enterococcus faecium and Streptococcus surrogates, Staphylococcus aureus, Klebsiella pneumoniae, Acitenobacter baumanii, Pseudomonas aeruginosa, and Enterobacter species along with the emerging nosocomial Escherichia strains). This study was performed to decipher the bacterial community structure through Illumina high throughput 16S rRNA gene sequencing, and to determine the resistance profile using the Sensititre antimicrobial susceptibility test (AST) conforming to clinical lab standards (NCCLS). Out of 1747 bacterial strains detected from wastewater influent and effluent, Pseudomonas was the most predominant genus related to ESKAPE in influent, with sequence reads corresponding to 21.356%, followed by Streptococcus (6.445%), Acitenobacter (0.968%), Enterococcus (0.063%), Klebsiella (0.038%), Escherichia (0.028%), and Staphylococcus (0.004%). Despite the different treatment methods used, the effluent still revealed the presence of some Pseudomonas strains (0.066%), and a wide range of gram-positive cocci, including Staphylococcus (0.194%), Streptococcus (0.63%) and Enterococcus (0.037%), in addition to gram-negative Acitenobacter (0.736%), Klebsiella (0.1%), and Escherichia sub-species (0.811%). The AST results indicated that the strains Escherichia along with Klebsiella and Acitenobacter, isolated from the effluent, displayed resistance to 11 antibiotics, while Pseudomonas was resistant to seven antibiotics, and Streptococcus along with Staphylococcus were resistant to 9 antibiotics. Results herein, proved the existence of some nosocomial MDR pathogens, known for ESKAPE, with potential drug resistance transfer to the non-pathogen microbes, requiring targeted remediation. Development of a Solid-State Monitoring System for Real-Time Monitoring of Florida Firefighter Exposure to Polyaromatic Hydrocarbons  
[PERSON] \({}^{1,5,6}\), [PERSON] \({}^{1,2}\), [PERSON] \({}^{1}\), [PERSON] \({}^{4}\), [PERSON] \({}^{1,6}\), [PERSON] \({}^{4}\), [PERSON] \({}^{4}\), [PERSON] \({}^{4}\), [PERSON] \({}^{4}\), [PERSON] \({}^{1,4,6}\) and [PERSON] \({}^{1,5,6}\)  
\({}^{1}\) Department of Biochemistry and Molecular Biology, University of Miami, Miller School of Medicine, Miami, FL 33136, USA  
\({}^{2}\) Department of Chemistry, College of Arts and Sciences, University of Miami, Coral Gables, FL 33146, USA  
\({}^{3}\) Department of Biomedical Engineering, College of Engineering, University of Miami, Coral Gables, FL 33146, USA  
\({}^{4}\) Department of Public Health Sciences, University of Miami, Miller School of Medicine, Miami, FL 33136, USA  
\({}^{5}\) Sylvester Comprehensive Cancer Center, University of Miami, Miller School of Medicine, Miami, FL 33136, USA  
\({}^{6}\) Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute, University of Miami, Miami, FL 33136, USA  
Studies on firefighters indicate a high incidence of cancer compared to the general populace. Previous studies using passive sampling devices report high rates of exposure to toxic compounds, including polyaromatic hydrocarbons (PAHs) during their work shift. Therefore, necessitating the need for real-time monitoring in order to reduce the risk of exposure. At present, no readily available real-time methods exist for determining the presence of these carcinogenic compounds in the field. We designed a portable sensor array as a detection system based on modified commercial sensors. Laboratory sensor validation was performed using PAH spike tests within airtight chambers. Subsequently, sensors were field tested in controlled live fire situations. Sensor array responses were measured at varying distances from outside the hot zone of a controlled burn in order to simulate firefighter exposure.