## 3 Results
### Changes in Volatile Compounds Generated from Simulated FA Oxidation System
\begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{2}{*}{**Number**} & \multirow{2}{*}{**Compounds**} & \multicolumn{3}{c}{**Volatile Compounds Concentration (ug/L)**} \\ \cline{3-5}  & & **Blank** & **C16:0** & **C22:6n3 + LOX** \\ \hline
1 & Quinoline, 1,2-dihydro-2,2,4-trimethyl- & 0.00 \(\pm\) 0.00 a & 0.00 \(\pm\) 0.00 a & 15.01 \(\pm\) 1.44 b \\
2 & Cyclohexane, butyl- & 0.00 \(\pm\) 0.00 a & 22.42 \(\pm\) 0.83 b & 24.78 \(\pm\) 1.73 b \\
3 & trars-Decalin, 2-methyl- & 0.00 \(\pm\) 0.00 a & 0.00 \(\pm\) 0.00 a & 10.36 \(\pm\) 1.14 b \\
4 & Decane & 0.00 \(\pm\) 0.00 a & 340.40 \(\pm\) 25.88 b & 293.78 \(\pm\) 28.78 b \\
5 & Dodecane & 22.15 \(\pm\) 0.81 a & 23.68 \(\pm\) 0.97 ab & 29.24 \(\pm\) 4.16 b \\
6 & Tetradeace & 5.10 \(\pm\) 0.08 b & 5.30 \(\pm\) 0.87 b & 2.88 \(\pm\) 0.62 a \\
7 & Undeaceane & 5.10 \(\pm\) 0.07 a & 296.20 \(\pm\) 35.44 b & 336.95 \(\pm\) 37.62 b \\
8 & Undeace, 2,6-dimethyl- & 0.00 \(\pm\) 0.00 a & 0.00 \(\pm\) 0.00 a & 4.94 \(\pm\) 1.08 b \\
9 & Tridecan & 1.12 \(\pm\) 0.07 a & 3.67 \(\pm\) 0.66 c & 2.72 \(\pm\) 0.08 b \\
10 & Cyclohexane, pentyl- & 8.95 \(\pm\) 0.10 a & 27.61 \(\pm\) 25.2 b & 28.22 \(\pm\) 3.51 b \\
11 & Benzene, 1,2,3,4-tetramethyl- & 0.00 \(\pm\) 0.00 a & 6.80 \(\pm\) 1.43 b & 16.68 \(\pm\) 2.90 c \\
12 & Benzene, 1-ethyl-2,3-dimethyl- & 0.00 \(\pm\) 0.00 a & 0.00 \(\pm\) 0.00 a & 24.93 \(\pm\) 2.54 b \\
13 & Benzene, 1-methyl-2-(1-methylethyl)- & 0.00 \(\pm\) 0.00 a & 0.00 \(\pm\) 0.00 a & 24.63 \(\pm\) 0.89 b \\
14 & Benzene, 2-ethyl-1,4-dimethyl- & 0.00 \(\pm\) 0.00 a & 0.00 \(\pm\) 0.00 a & 38.45 \(\pm\) 1.81 b \\
15 & P-Xylene & 0.00 \(\pm\) 0.00 a & 85.43 \(\pm\) 4.14 c & 77.14 \(\pm\) 2.69 b \\
16 & Toluene & 0.00 \(\pm\) 0.00 a & 0.00 \(\pm\) 0.00 a & 11.79 \(\pm\) 1.04 b \\
17 & Benzene, 1,3-dimethyl- & 0.00 \(\pm\) 0.00 a & 92.40 \(\pm\) 23.1 c & 78.76 \(\pm\) 2.73 b \\
18 & Mesityline & 0.00 \(\pm\) 0.00 a & 0.00 \(\pm\) 0.00 a & 16.28 \(\pm\) 3.59 b \\
19 & Benzene, 1,2,3-trimethyl- & 0.00 \(\pm\) 0.00 a & 0.00 \(\pm\) 0.00 a & 9.66 \(\pm\) 1.87 b \\
20 & o-Xylene & 0.00 \(\pm\) 0.00 a & 221.15 \(\pm\) 6.12 c & 100.60 \(\pm\) 5.17 b \\
21 & Ethlybenzene & 0.00 \(\pm\) 0.00 a & 95.34 \(\pm\) 7.28 c & 81.36 \(\pm\) 3.70 b \\
22 & Benzene, 1,2,3,5-tetramethyl- & 0.00 \(\pm\) 0.00 a & 29.43 \(\pm\) 3.19 b & 39.19 \(\pm\) 2.58 c \\
23 & Decanoic acid, methyl ester & 0.00 \(\pm\) 0.00 a & 248.19 \(\pm\) 7.81 b & 286.13 \(\pm\) 18.01 c \\
24 & Methyl tetradeaconate & 0.00 \(\pm\) 0.00 a & 0.00 \(\pm\) 0.00 a & 32.14 \(\pm\) 3.12 b \\
25 & Undeaconic acid, methyl ester & 0.00 \(\pm\) 0.00 a & 8.36 \(\pm\) 1.42 b & 7.99 \(\pm\) 1.16 b \\
26 & Octanoic acid, methyl ester & 0.00 \(\pm\) 0.00 a & 0.00 \(\pm\) 0.00 a & 105.71 \(\pm\) 1.70 b \\
27 & Dodecanoic acid, methyl ester & 0.00 \(\pm\) 0.00 a & 109.95 \(\pm\) 2.40 b & 207.83 \(\pm\) 38.88 c \\
28 & Hexadecenoic acid, methyl ester & 0.00 \(\pm\) 0.00 a & 0.00 \(\pm\) 0.00 a & 2.04 \(\pm\) 0.16 b \\
29 & Benzocyclheptatriene & 0.00 \(\pm\) 0.00 a & 3.08 \(\pm\) 0.15 b & 2.95 \(\pm\) 0.05 b \\
30 & Butylated Hydrovoluene & 1.25 \(\pm\) 0.22 a & 0.00 \(\pm\) 0.00 a & 7.03 \(\pm\) 1.04 b \\
31 & Naphthalene & 0.00 \(\pm\) 0.00 a & 23.34 \(\pm\) 1.34 b & 23.64 \(\pm\) 1.77 b \\ Total & & & & & \\ \hline \end{tabular}
\end{table} 
Table 8: Changes in the concentrations of volatile compounds produced from C22:6n3. when C18:In9c and C20:4 were used as substrates. Therefore, it was inferred that C18:In9c and C20:4 were suitable substrates for LOX. Herein, it was found that LOX activity was highest when C20:4 was used as a substrate, indicating that it was the optimal substrate for LOX. [PERSON] and [PERSON] [29] reported findings similar to those of this study. Although the total concentration of volatile compounds produced by LOX-treated C18:2n6c did not increase significantly, the concentration of the aldehydes, which was strongly associated with fish flavor, was 0.85 times higher than that produced via autoxidation, suggesting that LOX plays a vital role in promoting C18:2n6c as a flavor precursor.