## 3 Results and Discussion
### Volatile Compounds
#### 3.4.7 Acetate Esters
\begin{table}
\begin{tabular}{l c c c c c c c c c c} \hline \hline  & & & & \multicolumn{6}{c}{**Differences in Concentration**} \\ \cline{6-13}
**Co.** & **Volatile Compounds** & **ID** & **LRI\({}_{\text{e}}\)** & **LRI\({}_{\text{l}}\)** & \multicolumn{6}{c}{**One-Way ANOVA**} & \multicolumn{6}{c}{**Two-Way ANOVA**} \\ \cline{6-13}  & & & & **2021** & \multicolumn{6}{c}{**2022**} & \multicolumn{6}{c}{**Y**} & **H** & \(\mathbf{Y}\times\mathbf{H}\) \\ \cline{6-13}  & & & & **SCE** & **SPA** & **SCE** & **SPA** & & & & \\ \hline AE01 & Methyl acetate \(\ddagger\) & B, C & \(<\)1100 813 & 22.63 & 19.95 & **11.01 b** & **15.93 a** & n.s. & **21** & * \\ AE02 & Ethyl acetate (mg/L) \# & A, B & \(<\)1100 890 & **26.33 b** & **49.95 a** & 36.14 & 44.80 & **SPA** & n.s. & * \\ AE03 & Propyl acetate & B, C & \(<\)1100 982 & 43.93 & 20.79 & n.d. & n.d. & - & - & - \\ AE04 & Isobutyl acetate \(\ddagger\) & A, B, C & \(<\)1100 1009 & 111.5 & 126.1 & **79.97 b** & **91.04 a** & **SPA** & **21** & n.s. \\ AE05 & Butyl acetate \(\ddagger\) & B, C & \(<\)1100 1064 & **16.96 b** & **41.00 a** & 23.32 & 26.85 & **SPA** & n.s. & * \\ AE06 & Isomyl acetate (mg/L) \(\ddagger\) & A, B, C & 1133 1133 & **6.63 a** & **5.34 b** & **4.86 a** & **4.41 b** & **SCE** & **21** & * \\ AE07 & Pently acetate & B, C & 1169 1185 & 8.29 & 7.57 & n.d. & n.d. & - & - & - \\ AE08 & 3-Methyl-1-3-buten-1-yl acetate & B, C & 1190 1190 & n.d. & n.d. & **0.33 a** & **0.24 b** & - & - & - \\ AE09 & _trans_-2-penten-1-yl acetate & B & 1220 1228 & n.d. & n.d. & **0.32 a** & **0.17 b** & - & - & - \\ AE10 & Heyl acetate (mg/L) \(\ddagger\) & A, B, C & 1272 1272 & 0.50 & 0.46 & 0.30 & 0.27 & n.s. & **21** & n.s. \\ AE11 & _cis_-3-Hexenyl acetate \(\ddagger\) & B, C & 1314 1308 & 185.6 & 198.5 & **57.03 a** & **43.88 b** & n.s. & **21** & n.s. \\ AE12 & _trans_-3-Hexenyl acetate \(\ddagger\) & B, C & 1320 1315 & 132.9 & 141.2 & **54.76 a** & **42.86 b** & n.s. & **21** & n.s. \\ AE13 & 3-Ethoxypropyl acetate & B & 1361 & - & **11.88 a** & **0.15 b** & **11.04 a** & **0.36 b** & **SCE** & n.s. & n.s. \\ AE14 & Hepyl acetate & B, C & 1378 1374 & n.d. & n.d. & n.d. & 1.47 & 1.25 & - & - & - \\ AE15 & 3-Methylmethyl acetate & B, C & 1385 1395 & 0.85 & 0.75 & 3.55 & 3.48 & n.s. & **22** & n.s. \\ AE16 & 2-Ethyl-1-hexyl acetate & B & 1480 & - & 14.84 & 15.41 & 1.50 & 1.32 & n.s. & **21** & n.s. \\ AE17 & _cis_-2-tert-Butylcyclohexyl acetate & B & 1570 & - & 5.61 & 0.96 & 0.41 & 0.35 & n.s. & n.s. \\ AE18 & _trans_-2-Octadienyl acetate & B & 1570 & - & 0.26 & 0.22 & n.d. & n.d. & - & - & - \\ AE19 & _cis_-6-Nomom-1-yl acetate & B, C & 1634 1634 & 0.85 & 0.58 & n.d. & n.d. & - & - & - \\ AE20 & _cis_-4-tert-butylcyclohexyl acetate & B, C & 1688 1675 & 0.44 & 0.58 & 0.14 & 0.15 & n.s. & **21** & n.s. \\ AE21 & Diol acetate n.i. & B & 1741 & - & **44.51 a** & **17.30 b** & **31.00 a** & **13.12 b** & **SCE** & **21** & n.s. \\ AE22 & 1,3-Butaneediol diacetate & B, C & 1785 1768 & 3.71 & 1.67 & n.d. & n.d. & - & - & - \\ AE23 & 2-Phenethyl acetate (mg/L) \(\ddagger\) & A, B, C & 1803 1801 & 0.45 & 0.49 & **0.26 a** & **0.20 b** & n.s. & **21** & n.s. \\ \hline \hline \end{tabular} Abbreviations Co.—compound’s code; ID—identification of compounds: A—retention time accordant with that of a pure standard: B—mass spectra accordant with that from NIST 2.0, Wiley 8, and FFNSC 2 mass spectra electronic libraries or literature; C—linear retention index (LRI) accordant with an index from literature (compounds with only B in the ID column were considered tentatively identified); LRI\({}_{\text{e}}\)—experimental linear retention index; LRI\({}_{\text{l}}\)—linear retention index from literature; n.s.—not significant; n.d.—not detected. One-way ANOVA section: different superscript lowercase letters represent statistically significant differences between the two investigated wines determined by one-way ANOVA at \(p\) < 0.05 for each harvest year separately. Two-way ANOVA section: designations are reported representing yeast (SCE, SPA) and harvest year (21, 22) with higher concentration determined by two-way ANOVA at \(p\) < 0.05 with yeast (Y) and harvest year (H) as factors. An asterisk indicates a significant interaction between the two factors (Y \(\times\) H). Significant differences are highlighted in bold.  
\end{table}