Therefore, it can be considered as a method for bacteria delivery and release at a designated site in the body. [PERSON] et al. used electrospray to fabricate hydrogel microspheres for encapsulation of probiotics (i.e., _Lactobacillus plantarum_) [17]. Thus, the electrospinning technology is a versatile and straightforward platform to have fiber meshes combined with particle systems [12]. A different approach is represented by three-dimensional (3D) printing, in which bacteria could be encapsulated in polymeric inks ejected via a miniaturized nozzle through the application of a pressure. 3D printing is indeed a valuable approach to fabricate 3D structures for different biomedical applications (e.g., patches for wound healing). On the one hand, 3D printing might solve the problem related to the swelling of fibers observable in electrospinning applications by a careful control of the process parameters (i.e., temperature, deposition velocity, viscosity of the ink) [18]. However, the size of the printed filaments and the weight of the produced structures would be higher than those obtainable via electrospinning, which could limit eardrum hearing function. Among water soluble polymers, necessary to support cell viability, natural polysaccharides are outstanding figures, as they can retain very large amounts of water useful for the embodied cells, and are entitled with low immunogenicity and high biocompatibility towards different tissues of the body [19; 20]. A key example is alginic acid, a natural anionic polysaccharide derived from brown seaweeds (i.e., _Pracophyceae_), which has gained a long-term growing appreciation in drug delivery systems and other biomedical applications due to its key properties, including its structural similarity to the amorphous extracellular matrix in tissues [21]. Alginic acid sodium and potassium salts, known as alginates, are block-copolymer polysaccharides of L-gluturonic (G) and D-manuronic (M) acid residues connected by 1:4 glycoside linkages. Their crosslinking occurs via an exchange of monovalent cations (e.g., Na\({}^{+}\)) to bivalent cations (e.g., Ca\({}^{2+}\)), causing hydrogel formation, which is ideal to entrap live cells and biomolecules [22]. Some examples of alginate-based platforms for bacterial encapsulation have been reported in the literature. [PERSON] et al. developed alginate-based nanofibers that could potentially serve as a biocompatible and edible system for the safe delivery of live bacteria into the gut [23]. However, due to the strong entanglement of alginate chains, electrospun continuous fibers were obtained using a synthetic hydrophilic polymer as a carrier, such as PEO or PVA, usually in a relevant amount with respect to sodium alginate (SA) [15; 16]. The present work is focused on fabricating SA probiotic electrospray/electrospun fiber patch as a tool to possibly treat middle ear infections via bacterotherapy. In particular, the electrospinning technique was exploited to produce SA-based microparticles containing probiotics embedded in SA-based fibrous meshes, as a supportive substrate for the topical application in the tympan membrane. To reach this goal, electrospray and electrospinning processes needed the alginate-based formulations to be tuned. We thus investigated the rheological properties of the SA-based solutions and the morphology of the fibers and probiotic-loaded versus unloaded microparticles. Moreover, size variation upon crosslinking, swelling and biodegradation in physiological-like medium, cytocompatibility towards epithelial cells and antimicrobial activity towards a model pathogen, _E. coli_, were studied.