# Halophyte _Artemisia caerulescens_ L.: Metabolites from In Vitro Shoots and Wild Plants
## 2 Results and Discussion
### Phytochemical Investigation
#### 2.3.2 Essential Oil Hydrodistillation
2** & **Class** & \multicolumn{2}{c}{**Young**} & **Ripe** \\  & & & & **L Leaves** & **Infloresences** & **Infloresences** \\ \hline ethyl isovalerate & 859 & 858 & nt & -3  & 0.1 \(\pm\) 0.03 & - \\ \(\alpha\)-pinene & 933 & 939 & mh & - & 0.2 \(\pm\) 0.03 & 0.1 \(\pm\) 0.00 \\ sabinene & 973 & 975 & mh & 0.7 \(\pm\) 0.18 & 1.3 \(\pm\) 0.01 & 1.2 \(\pm\) 0.02 \\ p-cymene & 1025 & 1025 & mh & 0.2 \(\pm\) 0.04 & - & 0.3 \(\pm\) 0.03 \\ l-cineole & 1031 & 1031 & om & 0.3 \(\pm\) 0.01 & 0.3 \(\pm\) 0.02 & 0.3 \(\pm\) 0.01 \\ \(\gamma\)-terpinene & 1058 & 1060 & mh & - & 0.1 \(\pm\) 0.01 & 0.1 \(\pm\) 0.01 \\ _cis_-sabinene hydrate & 1066 & 1070 & om & 0.3 \(\pm\) 0.04 & 0.3 \(\pm\) 0.02 & 0.4 \(\pm\) 0.05 \\ _trans_-sabinene hydrate & 1098 & 1098 & om & 0.1 \(\pm\) 0.03 & 0.2 \(\pm\) 0.01 & 0.5 \(\pm\) 0.04 \\ \(\alpha\)-thujone & 1107 & 1102 & om & 51.5 \(\pm\) 0.03 & 39.7 \(\pm\) 1.81 & 41.7 \(\pm\) 0.78 \\ filifolome & 1108 & 1109 & om & 1.6 \(\pm\) 0.54 & - & - \\ g-thujone & 1117 & 1114 & om & 9.3 \(\pm\) 1.45 & 12.0 \(\pm\) 0.07 & 9.9 \(\pm\) 0.27 \\ dehydrosabinaketone & 1119 & 1121 & om & - & 0.2 \(\pm\) 0.05 & - \\ chrysanthenen & 1126 & 1128 * & om & 9.5 \(\pm\) 1.24 & 8.1 \(\pm\) 1.23 & 6.6 \(\pm\) 0.02 \\ _trans_-pinocavreol & 1139 & 1139 & om & 0.5 \(\pm\) 0.05 & 0.4 \(\pm\) 0.03 & 1.2 \(\pm\) 0.07 \\ pinocarone & 1163 & 1165 & om & 0.3 \(\pm\) 0.04 & 0.2 \(\pm\) 0.02 & 1.0 \(\pm\) 0.01 \\ 4+terpineol & 1177 & 1177 & om & 0.4 \(\pm\) 0.08 & 0.4 \(\pm\) 0.00 & 0.3 \(\pm\) 0.00 \\ myrtrenal & 1194 & 1196 & om & 0.1 \(\pm\) 0.02 & 0.1 \(\pm\) 0.00 & - \\ piperitone & 1254 & 1253 & om & 0.2 \(\pm\) 0.05 & - & - \\ _cis_-chysmsthenyl acetate & 1262 & 1265 & om & - & 0.4 \(\pm\) 0.03 & 0.8 \(\pm\) 0.01 \\ _is_piperitone & 1271 & 1272 * & om & 0.2 \(\pm\) 0.03 & 0.1 \(\pm\) 0.02 & - \\ _trans_-sabinyl acetate & 1294 & 1291 & om & - & 3.1 \(\pm\) 0.41 & 2.0 \(\pm\) 0.09 \\ \(\beta\)-caryophyllene & 1419 & 1419 & sh & - & - & 0.3 \(\pm\) 0.02 \\ germacrene D & 1481 & 1485 & sh & 0.5 \(\pm\) 0.27 & 0.8 \(\pm\) 0.15 & - \\ g-seline & 1486 & 1490 & sh & - & 1.0 \(\pm\) 0.07 & 1.7 \(\pm\) 0.14 \\ phenylethyl 3-methylbutyrate & 1491 & 1490 & nt & 0.3 \(\pm\) 0.20 & - & - \\ \(\alpha\)-cadinol & 1654 & 1654 & os & - & 0.1 \(\pm\) 0.02 & - \\ brevitolin & 1669 & 1675 & pp & 23.6 \(\pm\) 1.21 & 27.9 \(\pm\) 2.21 & 28.8 \(\pm\) 1.07 \\ mustakone & 1687 & 1676 & os & - & 0.3 \(\pm\) 0.01 & - \\ Eudesma-4(15),7-dien-1\(\beta\) -ol & 1688 & 1688 & os & - & 0.3 \(\pm\) 0.04 & - \\ g-notokatol & 1712 & 1712 & os & - & 0.3 \(\pm\) 0.01 & - \\ (\(\beta\)-lancool & 1762 & 1761 & os & - & 1.0 \(\pm\) 0.14 & - \\ methyl isocostate & 1792 & 1791 & os & - & 0.7 \(\pm\) 0.21 & - \\ kaurene & 2048 & 2043 & dh & - & 0.2 \(\pm\) 0.01 & - \\ \hline Chemical Classes & & & & \multicolumn{2}{c}{Young} & \multicolumn{2}{c}{Ripe} \\  & & & & & \multicolumn{2}{c}{(Blosson)} & \multicolumn{2}{c}{Infloresences} \\ \hline Monoterpene hydrocarbons (mh) & & & 0.9 \(\pm\) 0.22  & & 1.7 \(\pm\) 0.03  & 1.8 \(\pm\) 0.05  \\ Oxygenated monoterrenes (om) & & & 74.2 \(\pm\) 2.01  & 65.4 \(\pm\) 2.74  & 64.7 \(\pm\) 1.02  \\ Sesquirepene hydrocarbons (sh) & & & 0.5 \(\pm\) 0.27  & 1.7 \(\pm\) 0.22  & 2.0 \(\pm\) 0.16  \\ Oxygenated sesquiterpenenes (os) & & & - & 2.7 \(\pm\) 1.59 & - \\ Phenrypropropanoids (pp) & & & 23.6 \(\pm\) 1.21  & 27.9 \(\pm\) 2.21  & 28.8 \(\pm\) 1.07  \\ Other non-terpene derivatives (nt) & & & 0.3 \(\pm\) 0.20 & 0.1 \(\pm\) 0.03 & - \\ \hline Total identified (\%) & & & 99.5 \(\pm\) 0.12 & 99.7 \(\pm\) 0.00 & 97.3 \(\pm\) 0.15 \\ \hline OE hydrodistillation yield (\% w/w\)) & & 0.35 \(\pm\) 0.01  & 0.17 \(\pm\) 0.01  & 0.41 \(\pm\) 0.01 [FOOTNOTE:2]Footnotegenetic expression [44]. Unsurprisingly, since the metabolism was shifted to the production of thujones, the analysed samples showed low amounts of sabinene, ranging from 0.7 to 1.3% of the whole EO compositions. Today, thujones are the focus of debate concerning their effect on human health.