## 3 Results
### Effect of Room Temperature Storage
The total VOC accumulation from each specimen type across the 6-week period can be seen in Figure 9. The average VOC accumulation for the open SLF eggs was 87,859,910.5, while the average VOC accumulation for the closed SLF eggs was 279,285,727. The average VOC accumulation for the open crickets was 767,272,590, while the average VOC accumulation for the closed crickets was 1,085,909,475. The crickets had more VOC accumulation than the SLF eggs; however, around week 3, the abundance of the open crickets, closed eggs, and closed crickets began to even out. The highest abundance in the open eggs reached a value of 331,574,630, and the lowest abundance in the open eggs reached a value of 26,943,406.7. The highest abundance in the closed eggs reached a value of 582,760,430, and the lowest abundance in the closed eggs reached a value of 63,931,942. The highest abundance in the open crickets reached a value of 1,953,981,346, and the lowest abundance in the open crickets reached a value of 318,078,001. Finally, the highest abundance in the closed crickets reached a value of 1,815,987,183, and the lowest abundance in the closed crickets reached a value of 408,531,145. Figure 8: Stacked bar graph representing the relative peak area response of the odor profiles of the open and closed SLF eggs and crickets over a 6-week sampling period. A principal component analysis (PCA) was conducted to display the clustering components of the primary compounds from the open egg and cricket samples across the 6-week sampling period, as seen in Figure 10. PCA multivariate analysis showed that the first principal component accounted for 29.3% of the variability in the data, while the second principal component accounted for 18% of the variability in the data in the open samples. There is clustering seen in weeks 1 through 4 of the eggs, as well as in weeks 3 and 4 in the crickets. Week 5 of the eggs is grouped independently in its own quadrant, while week 0 is in the same quadrant as the cluster of weeks 1 through 4. In the crickets, weeks 0 and 1 are grouped in the same quadrant as the cluster of weeks 3 and 4, and weeks 2 and 5 are grouped in the same quadrant with each other. PCA multivariate analysis showed that the first principal component accounted for 37.6% of the variability in the data, while the second principal component accounted for 18.4% of the variability in the data in the closed samples. There is clustering seen in weeks 0 through 3, as well as week 5 in the egg samples. Week 4 in the eggs is not clustered, although it is in the same quadrant as the rest of the weeks. In the crickets, there is no clustering seen; however, weeks 1 through 5 are all grouped in the same quadrant, and weeks 4 and 5 are grouped very near each other. Week 0 in the crickets is grouped independently in its own quadrant. While the PCA depicted a lack of odor profile reproducibility over time for both lid conditions in the two insect species analyzed, an analysis of variance (ANOVA) was further conducted to statistically evaluate the effect of lid conditions on the overall volatile odor accumulation. ANOVA was used to test the significance of the primary compounds detected in this study as a function of specific targeted variables, in this case, sample lid condition. Figure 11 shows the distribution of the VOC accumulation based on lid sample condition (c--closed, o--open) for both SLF eggs and crickets. Setting the threshold for significance at \(p<0.05\), the SLF egg samples depicted a statistically significant effect at \(p=0.0005\), while the cricket samples yielded a non-significant effect at \(p\)= 0.0574. Figure 11 summarizes the distribution of VOC accumulation across both tested species. Figure 9: Total VOC accumulation graph from each specimen, open and closed SLF eggs and crickets, across the 6-week sampling period.