## 2 Results
### The Correlation between Environmental Factors and Ginensosides and Gene Expression

Table 7: Correlation analysis between environmental factors and ginensosides in roots at different growth times. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & **Temperature** & **PAR** & **Relative Humidity** & **Rain** & **Soil Water** \\  & **(\({}^{\circ}\)C)** & **(\(\mu\)E)** & **Humidity (\%)** & **(mm)** & **Potential (J/g)** \\ \hline Rg1 & 0.40 & \(-\)0.44 & 0.89 ** & 0.26 & \(-\)0.79 * \\ Re & 0.53 & \(-\)0.43 & 0.94 ** & 0.43 & \(-\)0.67 \\ Rf & 0.66 & \(-\)0.27 & 0.89 ** & 0.51 & \(-\)0.64 \\ Rb1 & 0.56 & \(-\)0.28 & 0.86 ** & 0.36 & \(-\)0.76 * \\ Rc & 0.41 & \(-\)0.67 & 1.00 ** & 0.52 & \(-\)0.44 \\ Rb2 & 0.70 & \(-\)0.43 & 0.93 ** & 0.83 ** & \(-\)0.17 \\ Rb3 & 0.41 & \(-\)0.35 & 0.83 ** & 0.21 & \(-\)0.84 ** \\ Rd & 0.45 & \(-\)0.24 & 0.89 ** & 0.31 & \(-\)0.77 \\ Ro & \(-\)0.09 & 0.60 & \(-\)0.81 ** & 0.00 & 0.83 ** \\ PPT & \(-\)0.03 & 0.93 ** & \(-\)0.84 ** & \(-\)0.40 & 0.07 \\ PPD & 0.73 * & 0.54 & 0.23 & 0.26 & \(-\)0.59 \\ Total saponins & 0.73 * & 0.04 & 0.69 * & 0.42 & \(-\)0.72 * \\ \hline \hline \end{tabular}  
* indicates a significant correlation (\(p<\) 0.05). ** indicates a significant correlation (\(p<\) 0.01).  
\end{table} 
Table 8: Correlation analysis between environmental factors and ginensosides in leaves at different growth times. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & **Temperature** & **PAR** & **Relative Humidity** & **Rain** & **Soil Water** \\  & **(\({}^{\circ}\)C)** & **(\(\mu\)E)** & **Humidity (\%)** & **(mm)** & **Potential (J/g)** \\ \hline HMGR & \(-\)0.24 & 0.82 ** & \(-\)0.96 ** & \(-\)0.47 & 0.30 \\ SS & 0.62 & 0.94 ** & \(-\)0.35 & 0.21 & \(-\)0.02 \\ FPS & \(-\)0.64 & 0.50 & \(-\)0.94 ** & \(-\)0.81 ** & 0.13 \\ SE & 0.82 ** & 0.73 * & \(-\)0.12 & 0.65 & 0.33 \\ DS & \(-\)0.19 & \(-\)0.91 ** & 0.78 * & \(-\)0.03 & \(-\)0.52 \\ PPDS & 0.27 & \(-\)0.03 & \(-\)0.02 & 0.67 & 0.91 ** \\ PPTS & 0.28 & \(-\)0.81 ** & 0.93 ** & 0.57 & \(-\)0.13 \\ \(\beta\)-AS & \(-\)0.21 & 0.50 & \(-\)0.82 ** & \(-\)0.07 & 0.86 ** \\ OAS & 0.36 & \(-\)0.33 & 0.79 * & 0.14 & \(-\)0.88 ** \\ \hline \hline \end{tabular}  
* indicates a significant correlation (\(p<\) 0.05). ** indicates a significant correlation (\(p<\) 0.01).  
\end{table} 
Table 9: Correlation analysis between environmental factors and gene expression in roots at different growth times. In the roots, HMGR was significantly positively correlated (\(p<0.01\)) with Re, Rf, and Rb3 (Table 11). SS was significantly positively correlated (\(p<0.01\)) with Rg1 and PPT-type ginseng. FPS was significantly positively correlated (\(p<0.01\)) with Re. SE was significantly positively correlated (\(p<0.01\)) with PPT-type ginsenosides. DS was significantly positively correlated (\(p<0.01\)) with Re, Rf and PPT-type ginsenosides. SS, FPS, SE, DS, PPDS, \(\beta\)-AS, and OAS gene expression was significantly correlated with ginsenosides. It is indicated that SS, FPS, SE, DS, PPDS, \(\beta\)-AS, and OAS genes have synergistic regulation roles in leaves (Table 12). The expression of a key enzyme gene in ginsenoside synthesis may only act on a certain site in the ginsenoside synthesis pathway. Due to multiple regulatory sites in the ginsenoside synthesis pathway. There are multiple steps between saponin synthesis, so not all genes have a significant correlation with saponin content.