## 4 Conclusions  
The effect of extraction temperature on the bioactivity of herbal infusions is very important. This study provides results regarding total phenolic content, antioxidant capacity, and toxicity of aqueous extracts of chamomile and sage at different extraction temperatures. All the examined assays of bioactivity indicated influence of the extraction temperature. The highest total phenolic content and maximum antioxidant capacity of aqueous extracts of chamomile and sage at tested temperatures (25, 80, and 100 \({}^{\circ}\)C) were achieved at 80 \({}^{\circ}\)C. Toxicity of both herbal aqueous extracts, causing bioluminescence inhibition of _V. fischeri_, was increased along with extraction temperature. Chamomile showed higher capacity to inhibit bioluminescence in comparison with sage. For all the aqueous herbal extracts, total phenolic content was significantly correlated with antioxidant activity; however, there was no link between toxicity and these two examined assays. The analyses of phenolic profiles of both aqueous extracts at 80 \({}^{\circ}\)C confirmed the presence of different flavonoids and phenolic compounds. The dominant compounds of chamomile aqueous extract were rutin trihydrate, ferulic acid, chlorogenic acid, and apigenin7-O-glucose. Rosmarinic acid, salvianolic acid K, and luteolin-7-O-glucuronide were detected as the main phenolic compounds of sage aqueous extract. Eventually, the effect of not only the extraction temperature but also other parameters such as time of extraction and amount of solvent play an important role in the evaluation of the beneficial properties and for monitoring the safety of herbal infusions. Overall, and despite the need for further studies to elucidate the optimal \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Rt** **(**min)** & **\(\lambda\)max** &
\begin{tabular}{c} **Molecular** \\ **(nm)** \\ \end{tabular} & **Proposed Compound** & **Chamomile \%** & **Sage \%** \\ \hline
19.78 & 327 & 353 & Chlorogenic acid & \(12.6\pm 0.2\) & \\
22.22, 298, & 179 & Caffeic acid & \(2.9\pm 0.5\) & \\
23.53 & 242, 323 & 193 & Ferulic acid & \(13.3\pm 0.4\) & \\
24.44 & 325 & & Ni\({}^{2}\) & \(5.7\pm 0.2\) & \\
27.68 & 235, 309 & 163 & _p_-Cumaric acid & & \(0.42\pm 0.05\) \\
23.21 & 231, 256, & 463 & Myricitrin & & \(1.6\pm 0.4\) \\
352 & & & cis 3.5-O-Dicaffeolyquinic acid\({}^{1}\) & \(3.1\pm 0.2\) & \\
28.55 & 220, 326 & 515 & Luteolin-7-O-glucose & \(2.5\pm 0.1\) & \(0.37\pm 0.02\) \\
28.69 & 255, 349 & 447 & Luteolin-7-O-glucose & \(2.5\pm 0.1\) & \(0.37\pm 0.02\) \\
28.83 & 220, 328 & 515 & trans 3,5-O-Dicaffeolyquinic acid\({}^{1}\) & \(4.4\pm 0.05\) & \\
29.17 & 255, 356 & 609 & Rutin trihydrate & \(15.1\pm 0.1\) & \\
253, 286, & 717 & Salvianolic acid B & & \(4.1\pm 0.4\) \\
29.87 & 268, 337 & 593 & Luteolin-7-O-rutinoside\({}^{1}\) & & \(3.2\pm 0.4\) \\
30.75 & 348 & 461 & Luteolin-7-O-glucuronide\({}^{1}\) & & \(15\pm 1\) \\
31.19 & 229, 328 & & Ni\({}^{2}\) & \(3.1\pm 0.2\) & \\
31.72 & 236, & 555 & Salvianolic acid K\({}^{1}\) & & \(15.4\pm 0.4\) \\
32.89 & 243, 289, & 330 & 359 & Rosmarinic acid & \(15\pm 1\) \\
32.94 & 242, 267, & & & \\
33.62 & & 445 & Apigenin-7-O-glucose & \(9.1\pm 0.1\) & \\
33.74 & 235, 322 & & & \\
34.59 & 229, 273, & & & \\
35.62 & 238, 267, & & & \\
332 & & 473 & Apigenin-7-O-acetylglucose\({}^{1}\) & \(6.9\pm 0.2\) & \\
42.66 & 242, 254, & & & Ni\({}^{2}\) & \(5.2\pm 0.3\) & \\
312 & & & & \\
266, 324, & & & & \\
365 & & & & \\ \hline \hline \end{tabular} \({}^{1}\) Tentative identification. \({}^{2}\) Ni: Not identified.  
\end{table} 
Table 2: LC-DAD-MS characteristics and quantification (%) of phenolic compounds identified in chamomile and sage aqueous extracts prepared at 80 \({}^{\circ}\)C.  
infusion preparation of the tested herbal extracts, it is possible to conclude that aqueous extracts of chamomile and sage at 80 \({}^{\circ}\)C could be regarded as health-promoting antioxidant beverages but their use should be moderated to ensure safe consumption. Conceptualization, N.S.S. ; investigation, N.S.S.