## 3 Results and Discussion
### OPLS-DA Analysis of Soluble Compounds
\begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline  & **Peak** & **m/z \({}^{*}\)** & **rtf(s)** & **Adduct** & **Description** & **Ion Mode** & **VIP** & \multicolumn{2}{c}{**Fold Change**} \\ \hline Amino Acids & M2777870 & 277.14 & 869.79 & (M+H)\({}^{*}\) & L-Saccharopine & POS & 2.02 & 0.02 & 43.07 \\  & M231T71 & 231.17 & 70.60 & (M+H)\({}^{*}\) & Ile-Val & POS & 2.41 & 0.05 & 18.45 \\  & M104T96 & 104.07 & 95.71 & (M+H)\({}^{*}\) & acid & POS & 2.34 & 0.08 & 12.10 \\  & M114T5799 & 114.06 & 578.85 & (M-H)\({}^{*}\) & D-Proline & NEG & 2.40 & 0.21 & 4.69 \\  & M166T474 & 166.09 & 474.49 & (M+H)\({}^{*}\) & L-Phenylalanine & POS & 1.68 & 0.22 & 4.51 \\  & M1307569 & 130.05 & 569.13 & (M+H)\({}^{*}\) & acid & POS & 2.97 & 0.34 & 2.97 \\  & M148T764 & 148.06 & 763.66 & (M+H)\({}^{*}\) & L-Glutamate & POS & 2.15 & 2.34 & 0.43 \\  & M133T715 & 133.06 & 714.69 & (M+H)\({}^{*}\) & L-Asparagine & POS & 2.49 & 9.47 & 0.11 \\  & M134T773 & 134.04 & 773.14 & (M+H)\({}^{*}\) & L-Asparate & POS & 1.59 & 9.61 & 0.10 \\  & M151T389 & 151.03 & 389.12 & (M-H)\({}^{-}\) & Xanthine & NEG & 2.23 & 0.01 & 136.75 \\  & M111T150 & 111.02 & 149.91 & (M-H)\({}^{-}\) & Uracil & NEG & 4.68 & 0.01 & 76.95 \\  & M282T191 & 282.12 & 191.44 & (M+H)\({}^{*}\) & 2\({}^{*}\)-O- & POS & 4.45 & 0.02 & 63.31 \\  & M137T306 & 137.04 & 305.82 & (M+H)\({}^{*}\) & Hypoxanthine & POS & 2.29 & 0.05 & 18.39 \\  & M268T312 & 268.10 & 312.36 & (M+H)\({}^{*}\) & Adenosine & POS & 4.48 & 0.35 & 2.88 \\  & M136T290\_2 & 136.06 & 290.47 & (M+H)\({}^{*}\) & Adenine & POS & 3.52 & 10.98 & 0.09 \\ Esters & M115T60\_2 & 115.07 & 60.07 & (M+H-H\({}_{2}\)O)\({}^{*}\) & Ethyl & POS & 2.84 & 0.02 & 43.84 \\  & M101T243 & 101.02 & 243.28 & (M+H-H\({}_{2}\)O)\({}^{*}\) & Erythrono-1,4- & POS & 2.35 & 0.04 & 23.41 \\  & M117T78 & 117.05 & 78.45 & (M+H)\({}^{*}\) & Methyl acetoacetate & POS & 1.53 & 0.07 & 13.76 \\  & M343T58 & 343.05 & 58.15 & (M+H)\({}^{*}\) & Dinetylene-Bis & POS & 1.69 & 0.10 & 10.43 \\  & M207T123 & 207.05 & 123.33 & (M+CH\({}_{3}\)COO)\({}^{-}\) & D-Arabonno-1,4- & & & & \\  & M177T190 & 177.04 & 189.63 & (M-H)\({}^{-}\) & D-Glucono-1,5- & NEG & 2.37 & 1.94 & 0.51 \\  & M149T295 & 149.08 & 295.48 & (M+H)\({}^{*}\) & Dihydroxyfumarate & POS & 1.56 & 2.49 & 0.40 \\ Others & M146T350 & 146.12 & 350.27 & (M+H)\({}^{*}\) & Acetylcholine & POS & 2.64 & 0.02 & 54.78 \\  & M120T60 & 120.08 & 60.03 & (M+H\({}_{2}\)O)\({}^{*}\) & Tyramine & POS & 1.83 & 0.02 & 41.18 \\  & M449T302 & 449.11 & 301.56 & (M+H)\({}^{*}\) & Quercirtin & POS & 5.28 & 0.12 & 8.07 \\  & M287T79 & 287.09 & 79.20 & (M+H)\({}^{*}\) & 4\({}^{\prime}\),5-Dihydroxy-7- & POS & 1.56 & 0.15 & 6.53 \\  & M271T67 & 271.06 & 66.52 & (M-H)\({}^{-}\) & (-)-Narningen & NEG & 2.07 & 0.23 & 4.37 \\  & M317T128 & 317.07 & 127.75 & (M+H)\({}^{*}\) & Quercetin & POS & 1.64 & 0.24 & 4.23 \\  & M287T302 & 287.05 & 302.01 & (M+H)\({}^{*}\) & Kaempfer & POS & 2.05 & 0.24 & 4.20 \\  & M258T36 & 258.11 & 735.67 & M\({}^{*}\) & Glyoxrophosphcholine & POS & 5.52 & 0.25 & 3.93 \\  & M298T150 & 298.10 & 149.98 & (M+H)\({}^{*}\) & S-Methyl-5\({}^{*}\)- & POS & 6.10 & 0.26 & 3.78 \\  & M118T510\_2 & 118.09 & 510.29 & (M+H)\({}^{*}\) & Betaine & POS & 4.71 & 0.28 & 3.57 \\  & M104T494 & 104.11 & 494.32 & M\({}^{*}\) & Choline & POS & 10.66 & 0.35 & 2.90 \\  & M268T259 & 268.14 & 258.63 & (M+CH\({}_{3}\)CN+Na)\({}^{*}\) & Acetylcarnitine & POS & 1.71 & 1.93 & 0.52 \\  & M123T93 & 123.05 & 93.02 & (M+H)\({}^{*}\) & Nicotinamide & POS & 2.01 & 2.15 & 0.47 \\  & M449T479 & 449.11 & 479.35 & M\({}^{*}\) & Cyandin & POS & 3.82 & 2.92 & 0.34 \\  & M127T03 & 127.04 & 702.88 & (M+H)\({}^{*}\) & 13,5-Benzenetriol & POS & 2.28 & 11.93 & 0.08 \\ \hline \hline \end{tabular} \({}^{*}\)_m/z_: mass-to-charge ratio, rt(s): Retention time (s).  
\end{table} 
Table 4: _Cont._The differential expression of alcohols was analyzed, with more than half of them being sugar alcohol derivatives such as galactinol, xylitol, and tribitol.