## 3 Results
### Fusarium Fungi and Mycotoxins  
The total amount of _Fusarium_ fungi in pollen stored for 3 days with moisture content at 22.6%, as compared to the control, significantly increased on an average up to 17.03%. The diversity of _Fusarium_ species in fresh pollen was not high. Six _Fusarium_ species were identified (Table 4). After 5 h in the pollen studied, _F. graminearum_ and _F. sporotrichoides_ dominated, which were identified among 30.06% and 27.07% of all isolated _Fusarium_ genera, respectively. _F. culmorum_ (14.38%) and _F. oxysporum_ (13.11%) were the most common species and _F. poae_ (7.69%) and _F. verticilioides_ (7.69%) were classified as the least common species. During the study period, the number of isolates of most _Fusarium_ species were increasing with a longer time of pollen storage. After 3 days, increases in _F. graminearum_ (19.23%) and _F. sporotrichoides_ (15.92%) isolates were observed compared to the control. These species dominated throughout the study period (Figure 1). A positive strong correlation was found (r = 0.99, \(p\) < 0.05) between _F. graminerum_ and pollen storage time, and a weak (r = 0.95, \(p\) < 0.05) correlation was found between _F. sporotrichoides_ and storage time. The amounts of _F. poae_, _F. oxysporum_ and _F. verticilioides_ species increased by 4.82%, 2.17% and 0.8%, respectively. A very strong correlation (r= 0.95, \(p\) < 0.01) was found between _F. culmorum_ and storage time. \begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{2}{*}{**Fungi**} & \multicolumn{4}{c}{**Storage Duration**} \\ \cline{2-5}  & **After 5 h** & **After 1 Day** & **After 2 Days** & **After 3 Days** \\ \hline _F. culmorum_ & 1.1 \(\times\) 10\({}^{1}\)A b  & 1 \(\times\) 10\({}^{1}\)A b  & 1 \(\times\) 10\({}^{1}\)A b  & 1 \(\times\) 10\({}^{1}\)A b  \\ _F. gravsporum_ & 1.1 \(\times\) 10\({}^{1}\)A b  & 1.6 \(\times\) 10\({}^{2}\)B a  & 2 \(\times\) 10\({}^{2}\)A a  \\ _F. gravsporum_ & 1.1 \(\times\) 10\({}^{1}\)A b  & 1.6 \(\times\) 10\({}^{1}\)A b  & 1.1 \(\times\) 10\({}^{1}\)A b  \\ _F. gravsporum_ & 1.2 \(\times\) 10\({}^{1}\)A b  & 1.2 \(\times\) 10\({}^{1}\)A b  & 1.3 \(\times\) 10\({}^{1}\)A b  & 1.2 \(\times\) 10\({}^{1}\)A b  & 1.3 \(\times\) 10\({}^{1}\)A b  & 1.3 \(\times\) 10\({}^{1}\)A b  & 1.3 \(\times\) 10\({}^{1}\)A b  & 1.8 \(\times\) 10\({}^{1}\)A b  & 1.1 \(\times\) 10\({}^{1}\)A b  & 1.1 \(\times\) 10\({}^{1}\)A b  & 1.1 \(\times\) 10\({}^{1}\)A b  & 1.1 \(\times\) 10\({}^{1}\)A b  & 1.2 \(\times\) 10\({}^{1}\)A b [FOOTNOTE:b]Footnote b: Note: cfu g\({}^{-1}\)—colomy forming units per gram in bee pollen; the differences between the averages of treatments, marked by different letter (a, b) are significant (_p_ < 0.05). The results of mycotoxins (Table 5) indicate that the samples of bee pollen were contaminated by ZEN and DON mycotoxins. The average concentration of the toxins zearalenone (ZEN) and deoxynivalenol (DON) varied considerably, ranging from 65 to 280 ug kg\({}^{-1}\) and from 47 up to 120 ug kg\({}^{-1}\), respectively. A storage duration of 3 days influenced the highest concentrations of mycotoxins ZEN and DON. Contamination by the T-2 toxin in all bee pollen samples was found to be below the detection limit. On the day of pollen collection, 67 ug kg\({}^{-1}\) of mycotoxin zearalenone (ZEN) was detected. The pollen was most heavily contaminated with zearalenone (ZEN) after 3 days of storage, at 280 ug kg\({}^{-1}\), and it was the highest concentration of this mycotoxin found in all study variants. Mycotoxin deoxynivalenol (DON) at 47 ug kg\({}^{-1}\) in the pollen was found on the day of pollen collection. A significant increase in the concentration of deoxynivalenol (DON) was found after 3 days, comprising 120 ug kg\({}^{-1}\). A positive correlation (\(r\) = 0.55, \(p\) < 0.05) was ascertained between moisture content of the pollen and ZEN concentration. No correlation was found between ZEN and storage duration or the total amount of fungi.