## 3 Results
### Temperature Analysis
subtilis_ ATCC 6633, with an even bigger difference in the growth in comparison to the autoclave (61.80 \(\pm\) 20.01 CFU), as follows: 7 min (92.20 \(\pm\) 6.29 CFU; \(p\) < 0.001; [PERSON]), 10 min (88.93 \(\pm\) 7.20 CFU; \(p\) < 0.001; [PERSON]), 13 min (87.73 \(\pm\) 8.44 CFU; \(p\) < 0.001; [PERSON]), and 15 min (88.20 \(\pm\) 1.40 \(p\) < 0.001) (Figure 4). Figure 3: Bar graph showing the mean CFU obtained in SDA prepared with conventional sterilization (control, in an autoclave (or by boiling)), and in a microwave oven for 7 min (7\({}^{\prime}\)), 10 min (10\({}^{\prime}\)), 13 min (13\({}^{\prime}\)), and 15 min (15\({}^{\prime}\)), after inoculation using the pour plate method, for _C. albicans_, _S. cerevisiae_, and _A. brasiliensis_. For _L. monocytogenes_ ATCC 13932, although less growth in all media was reported, this trend was also observed, with significantly higher productivity in the microwave oven vs. the autoclave (35.60 \(\pm\) 23.37 CFU), as follows: at 7 min (45.47 \(\pm\) 1.36 CFU; \(p\) = 0.030; Tukey), 10 min (48.53 \(\pm\) 2.31 CFU; \(p\) = 0.002; [PERSON]), 13 min (44.20 \(\pm\) 1.40 CFU; \(p\) = 0.010; [PERSON]), and 15 min (46.60 \(\pm\) 1.11 CFU; \(p\) = 0.012; [PERSON]) (Figure 4). For _A. brasiliensis_ ATCC 16404, the lowest growth was observed at 7 min in the microwave oven (9.67 \(\pm\) 3.97 CFU). Significantly lower CFU counts were obtained in comparison to the autoclave (27.20 \(\pm\) 4.38 CFU; \(p\) < 0.001; [PERSON]) at the following times: 7 min (9.67 \(\pm\) 3.97 CFU), 10 min (12.53 \(\pm\) 5.21 CFU), and 13 min (14.33 \(\pm\) 4.50 CFU) (Figure 4). For two bacteria, _P. aeruginosa_ ATCC 9207 (reclassified as _Pseudomonas paraeruginosa_) and _C. perfringens_ ATCC 13124, higher productivity was observed using the autoclave vs. the microwave oven. For _P. aeruginosa_ ATCC 9207, a significantly higher CFU count was obtained in the autoclave in comparison to 15 min of exposure in the microwave oven (68.60 \(\pm\) 15.61 CFU vs. 57.13 \(\pm\) 9.58 CFU; \(p\) = 0.034; Tukey). The productivity in the media sterilized in the microwave oven decreased with the exposure times, as follows: 10 vs. 15 min (66.67 \(\pm\) 12.23 CFU vs. 57.13 \(\pm\) 9.58 CFU; \(p\) = 0.005; Tukey) and 13 vs. 15 min (65.13 \(\pm\) 5.67 CFU vs. 57.13 \(\pm\) 9.58 CFU; \(p\) = 0.026; GLM) (Figure 4). For _C. perfringens_ ATCC 13124, a gradual decrease in productivity was observed across the exposure times in the microwave oven, with statistically significant differences (_p_ = 0.009; Tukey) between the autoclave-sterilized media (52.60 \(\pm\) 2.70 CFU) and the media sterilized in the microwave oven for 15 min (27.40 \(\pm\) 21.33 CFU). Quality Control and Effect of Sterilization in a Microwave Oven vs. Boiling on the Productivity of VRBG  
For _E. coli_ ATCC 25922, _S._ ser. Typhimurium and _P. aeruginosa_, significantly higher growth in the medium exposed to the microwave was observed in comparison to the boiled media (Figure 5). For _S._ ser. Typhimurium ATCC 14028, the highest productivity was observed at 10 min (66.27 \(\pm\) 49.98 CFU), followed by 13 min (50.27 \(\pm\) 20.24 CFU), 7 min (40.27 \(\pm\) 12.88 CFU), and 15 min (36.07 \(\pm\) 21.99 CFU), with significant differences between the durations of 7 and 10 min (_p_ = 0.049; Tukey), and 10 and 15 min (_p_ = 0.009; Tukey). Figure 4: Bar graph showing the mean CFU obtained in TSA prepared with conventional sterilization (control, in an autoclave (or by boiling)), and in a microwave oven for 7 min (7), 10 min (10), 13 min (13), and 15 min (15), after inoculation using the pour plate method, for _E. coli_, _C. albicans_, _S. aureus_, _B. subtilis_, _L. monocytogenes_, _A. brasiliensis_, _P. aeruginosa_, _S._ ser. Typhimurium, and _C. perfringens_. For _E. coli_ ATCC 25922, similar CFU values were obtained at 7 min (116.67 \(\pm\) 26.95 CFU), 10 min (93.73 \(\pm\) 10.72 CFU), and 13 min (92.93 \(\pm\) 7.69 CFU), with a significant decrease at 15 min (65.80 \(\pm\) 9.61 CFU) vs. the rest of the times (7 vs. 15 min; \(p\)!