## 3 Results
### Basic Characteristic of Patients
\begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Variable** & **Total** **(_tr = 69_)** & **LG Copper Area—No** & **LG Copper Area—Yes** & _p_**-Value** \\ \hline Dietary supplements: & & & & \\ no & 46 (66.7) & 12 (60.0) & 34 (69.4) & 0.453 \\ yes & 23 (33.3) & 8 (40.0) & 15 (30.6) & \\ \hline Reason of extraction: & & & & \\ surgical & 54 (78.3) & 20 (100.0) & 34 (69.4) & \\ orthodontic & 8 (11.6) & 0 (0.0) & 8 (16.3) & \\ inflammation & 7 (10.1) & 0 (0.0) & 7 (14.3) & \\ \hline Vit. D3 (ng/mL) & 27.0 (19.8–29.5) & 27.8 (25.5–32.2) & 26.4 (18.5–28.4) & \\  & [22.5–32.1] & [24.9–37.6] & [20.9–31.8] & 0.135 \\ \hline Vit. D3 (ng/mL): & & & & \\ <20—big deficiency & 18 (26.1) & 3 (15.0) & 15 (30.6) & \\
20–29—deficiency & 34 (49.3) & 9 (45.0) & 25 (51.0) & 0.173 \\
30–50—norm & 11 (15.9) & 6 (30.0) & 5 (10.2) & \\
51–100—above the norm & 6 (8.7) & 2 (10.0) & 4 (8.2) & \\ \hline \hline \end{tabular}  
*: statistically significant.  
\end{table} 
Table 2: _Cont._ \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Variable** & **Total** **(_tr = 69_)** & **LG Copper Area** & **LG Copper Area** & _p_**-Value** \\ \hline Mn (\(\mathrm{ug/g}\)) & 0.19 (0.13–0.28) & 0.17 (0.14–0.25) & 0.22 (0.13–0.30) & \\  & [0.19–0.28] & [0.14–0.34] & [0.19–0.28] & 0.796 \\ \hline Cr (\(\mathrm{ug/g}\)) & 0.0 (0.00–0.00) & 0.00 (0.00–0.00) & 0.00 (0.00–0.00) & \\  & [0.03–0.16] & [0.00–0.29] & [0.03–0.14] & 0.667 \\ \hline Ni (\(\mathrm{ug/g}\)) & 0.0 (0.00–0.00) & 0.0 (0.00–0.00) & 0.00 (0.00–0.04) & \\  & [0.00–0.32] & [0.00–0.13] & [0.00–0.43] & 0.183 \\ \hline Cu (\(\mathrm{ug/g}\)) & 0.12 (0.05–0.24) & 0.08 (0.01–0.15) & 0.13 (0.06–0.28) & 0.103 \\  & [0.09–0.25] & [0.01–0.17] & [0.10–0.26] & 0.103 \\ \hline Fe (\(\mathrm{ug/g}\)) & 3.59 (1.82–5.88) & 1.81 (1.23–4.79) & 3.86 (2.51–6.23) & 0.016 * \\  & 13.56–6.14] & [123–6.03] & [3.82–6.28] & 0.016 * \\ \hline Cd (\(\mathrm{ug/g}\)) & 0.06 (0.05–0.09) & 0.06 (0.05–0.08) & 0.07 (0.05–0.09) & \\  & [0.05–0.09] & [0.05–0.08] & [0.05–0.09] & 0.117 \\ \hline Pb (\(\mathrm{ug/g}\)) & 0.47 (0.30–0.66) & 0.31 (0.22–0.48) & 0.53 (0.37–0.75) & \\  & [0.34–0.68] & [0.25–0.48] & [0.39–0.76] & 0.002 * \\ \hline Zn (\(\mathrm{ug/g}\)) & 100.36 (90.24–150.69) & 99.70 (81.40–114.95) & 103.80 (90.31–155.17) & \\  & [93.29–141.26] & [88.63–118.51] & [94.06–151.67] & 0.311 \\ \hline \hline \end{tabular}  
*: statistically significant.  
\end{table} 
Table 3: Tissue concentrations of toxic metals in the removed teeth in the total study group and two subgroups of patients, divided according to place of residence. Descriptive data are presented as median (Q1–Q3) and [95% CI]. Mann–Whitney test was used for analysis of differences between two subgroups of patients. The manganese content significantly depended on the occupation of the patients (\(p\) = 0.043). In the group of students, the median was 0.14 \(\mu\)g/g, while among the white-collar workers, the median was 0.25 \(\mu\)g/g--both of the results were statistically significant in the pair of subgroups (post hoc test). The high manganese content was determined as statistically significant in relation to increase of vitamin D3 concentration in capillary blood (\(p\) = 0.012). In the group of patients with 20-29 ng/mL of vitamin D3 concentration, the median of manganese content in the third molars was 0.13 \(\mu\)g/g, in the group of patients with 30-50 ng/mL of vitamin D3 concentration the median of manganese content in the third molars was 0.28 \(\mu\)g/g--both results were classified as statistically significant in the pair of subgroups (post hoc test). In the case of chromium and nickel concentration in the removed third molars, the occupation showed a tendency to statistical significance (\(p\) = 0.085 and \(p\) = 0.072, respectively). A statistically significant relationship between vitamin D3 concentration in the capillary blood and chromium content was shown (\(p\) = 0.034). The content of copper in the examined samples increased with the age of patients--values showed a tendency to statistical significance (\(p\) = 0.061).