## 3 Results and Discussion
### Identification of Phenolic Compounds by UHPLC-TOF/HRMS  
A targeted analysis, based on UHPLC-TOF/HRMS (ultra-high-performance liquid chromatography-time-of-flight high-resolution mass spectrometry), was conducted to identify the phenolic compounds present in the ethanol extracts, since the phenolic composition of _M. crystallinity_ is still not reported in the literature. Table 4 shows that the phenolic profile was diverse among the cotyledon, stem, and shoots of _M. crystallinity_. The 17 compounds detected in extracts of each organ of _M. crystallinum_ were salicaldehydeyde, cinnanate, feruloyl hexoside, sinapyl alcohol, sinapyl alcohol, mellein, roseo  
* Antiscorbtic factor, Vitamin C 2 EDTA: Ethylenediamineettetaectic acid solution. Means with different letters in each column (common letter (a-c): the level of significance for each part) and row (capital letter (A–E): the level of significance for each treatment with ppm) are significantly different by [PERSON]’s multiple range test (\(p<0.05\)). Values are mean \(\pm\) SD (n = 3). **\(p<001\), *** \(p<0.001\) compared to the control by ANOVA. \end{table}
Table 3: Hydroxyl radical scavenging activity and FE\({}^{2+}\) chelating activity with mean \(\pm\) S.D. (%) ranging from 62.5 ug/mL to 1000 ug/mL of ethanol extract from the cotyledon, stem, and leaf of _M. crystallinity_ L.  
side, 2-O-\(p\)-coumaroyl-D-glucose, 4\({}^{\prime}\)-O-b-d-glucosyl-5-O-methylvisamminol, cinnassiol A 19-glucoside, blumenol C glucoside, NNAL-\(N\)-glucuronide, isochinomin, 2,2\({}^{\prime}\)-dihydroxy-d-dimethoxyychalcone, 2\({}^{\prime}\)-hydroxy-3\({}^{\prime}\),4\({}^{\prime}\)-dimethoxyychalcone, and cinnassiol D2 glucoside. The main constituents of extracts obtained in this study were flavonoids, including their \(O\)-glycosides; nevertheless, compounds that have not been previously described were found in this study. The mass spectrometry stage analyses were performed in the positive and negative ionization modes. Interestingly, phenolic acid and flavonoid compounds contained in the different organs of _M.crystallinum_ L. were not previously reported. [PERSON] et al. (2016) analyzed the polyphenol compounds in the plants of the _Mesembryanthemum_ genus and reported the detection of phoretic, overcitin, avicularin, phoreticin xyloglucide, and rutin. Their identification in the cotyledon and stem extracts of the ice plant could encourage further investigation of promising sources of natural, biological, and active molecules. With the 17 compounds identified within the units, it would be advisable, using quantitative analysis, to select the organs of _M.crystallinum_ that provide the most nutritional content for the development of foods whose components fully demonstrate the body's regulatory functions related to biological defense. Among the three organs of _M.crystallinum_ observed during the research process, the organs consisting of the most flavonoids were the cotyledon and stem. The main compound classes, those that were detected only in the cotyledon and stem of the _M.crystallinum_ extracts, were epigallocatechin and 1-O-feruloylglucose. Epigallocatechin (otherwise known as green tea), or particularly, its major polyphenonic constituent, epigallocatechin-3-gallate (EGCG), has been shown to possess remarkable  
\begin{table}
\begin{tabular}{l c c c c c c c} \hline \hline  & **R.T** & **Analytic Name** & **Molecular** & **Molecular** & \multirow{2}{*}{**Adduct**} & \multicolumn{3}{c}{**Mass Error (ppm) (Mean \(\pm\) S.D. )**} \\  & **(min)** & & & **Formula** & **Weight** & & **Cotyledon** & **Shoot** & **Stem** \\ \hline
1 & 1.24 & Epigallocatechin & C\({}_{1}\)H\({}_{1}\)O\({}_{7}\) & 306.1 & [M-H]\({}^{*}\) & 8.82 \(\pm\) 0.03 & - & 8.03 \(\pm\) 0.03 \\
2 & 1.38 & Salicylicaldehyde & C\({}_{2}\)H\({}_{2}\)O\({}_{3}\) & 122.2  
cancer chemopreventive and therapeutic potential against various cancer sites in animal tumor bioassay systems and some human epidemiologic studies [27].