## 3 Results
\begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline
**Area** & **Date** & **Site** & \begin{tabular}{c} **Fruit** \\ **Ripeness** \\ \end{tabular} & \begin{tabular}{c} **Sample** \\ **Size (Fruit)** \\ \end{tabular} & \begin{tabular}{c} **SWD** \\ **SWD** \\ \end{tabular} & \
\begin{tabular}{c} **Non-** \\ **SWD** \\ \end{tabular} &
\begin{tabular}{c} **_Drosophila_** \\ **per Fruit** \\ \end{tabular} & \(\chi^{2}\) & \(p\) \\ \hline \multirow{8}{*}{October 2019} & \multirow{3}{*}{MON 1} & Ripe & 20 & 1070 & 50 & 56.0 & \multirow{3}{*}{147.76} & \multirow{3}{*}{<0.0001} \\ \cline{3-4}  & & Overripe & 10 & 418 & 135 & 55.3 & & \\ \cline{3-4}  & \multirow{3}{*}{MON 2} & Ripe & 20 & 103 & 43 & 7.3 & \multirow{3}{*}{10.67} & \multirow{3}{*}{0.0011} \\ \cline{3-4}  & & Overripe & 10 & 48 & 50 & 9.8 & & \\ \cline{3-4}  & \multirow{3}{*}{MON 3} & Ripe & 20 & 191 & 1 & 9.6 & \multirow{3}{*}{26.69} & \multirow{3}{*}{<0.0001} \\ \cline{3-4}  & & Overripe & 10 & 52 & 11 & 6.3 & & \\ \cline{3-4}  & \multirow{3}{*}{MON 1\({}^{\prime}\)} & Ripe & 40 & 18 & 0 & 0.5 & \multirow{3}{*}{N/A} & \multirow{3}{*}{N/A} \\ \cline{3-4}  & & Overripe & 20 & 6 & 0 & 0.3 & & \\ \cline{3-4} November 2019 & \multirow{3}{*}{MON 2\({}^{\prime}\)} & Ripe & 40 & 425 & 0 & 10.6 & \multirow{3}{*}{N/A} & \multirow{3}{*}{N/A} \\ \cline{3-4}  & & Overripe & 20 & 222 & 0 & 11.1 & & \\ \cline{3-4}  & \multirow{3}{*}{MON 3\({}^{\prime}\)} & Ripe & 40 & 6 & 0 & 0.2 & \multirow{3}{*}{0.00} & \multirow{3}{*}{1.0000} \\ \cline{3-4}  & & Overripe & 20 & 11 & 1 & 0.6 & & \\ \hline \multirow{8}{*}{Monterey  Bay} & \multirow{3}{*}{MON 4} & Ripe & 40 & 5 & 0 & 0.1 & \multirow{3}{*}{3.61} & \multirow{3}{*}{0.0574} \\ \cline{3-4}  & & Overripe & 20 & 17 & 22 & 2.0 & & \\ \cline{3-4}  & \multirow{3}{*}{MON 5} & Ripe & 40 & 76 & 0 & 1.9 & \multirow{3}{*}{152.18} & \multirow{3}{*}{<0.0001} \\ \cline{3-4}  & & Overripe & 20 & 28 & 149 & 8.9 & & \\ \cline{3-4}  & \multirow{3}{*}{MON 6} & Ripe & 40 & 476 & 2 & 12.0 & \multirow{3}{*}{11.60} & \multirow{3}{*}{0.0007} \\ \cline{3-4}  & & Overripe & 20 & 399 & 16 & 20.8 & & \\ \cline{3-4}  & \multirow{3}{*}{MON 7} & Ripe & 40 & 858 & 0 & 21.5 & \multirow{3}{*}{N/A} & \multirow{3}{*}{N/A} \\ \cline{3-4}  & & Overripe & 20 & 369 & 0 & 18.5 & & \\ \hline \multirow{8}{*}{MON 4\({}^{\prime}\)} & Ripe & 40 & 6 & 21 & 0.7 & \multirow{3}{*}{3.89} & \multirow{3}{*}{0.0485} \\ \cline{3-4}  & \multirow{3}{*}{MON 5\({}^{\prime}\)} & Ripe & 40 & 14 & 13 & 1.4 & \\ \cline{3-4}  & & Ripe & 40 & 3 & 0 & 0.1 & \multirow{3}{*}{1.11} & \multirow{3}{*}{0.2931} \\ \cline{3-4}  & & Overripe & 20 & 24 & 23 & 2.4 & & \\ \cline{3-4}  & \multirow{3}{*}{MON 6\({}^{\prime}\)} & Ripe & 40 & 955 & 49 & 25.1 & \multirow{3}{*}{55.87} & \multirow{3}{*}{<0.0001} \\ \cline{3-4}  & & Overripe & 20 & 1166 & 197 & 68.2 & & \\ \cline{3-4}  & \multirow{3}{*}{MON 7\({}^{\prime}\)} & Ripe & 40 & 1523 & 107 & 40.8 & & \\ \cline{3-4}  & & Overripe & 20 & 586 & 15 & 30.1 & & \\ \hline \end{tabular}
\end{table} 
Table 1: _Cont._Figure 2: Allelic discrimination results. The PCR reactions included fluorogenic probes to detect SNP associated with _Drosophila simulans_ (FAM-labeled probe) or _Drosophila suzukii_ (Cal Orange 560-labeled probe). Larval gDNA from _D. suzukii_ only (circle), _D. simulans_ only (square) or mix of both species (triangle) used as a template for internal controls are represented in black. The red and blue dashed lines are the RFU values for the standards with five _D. suzukii_ and one _D. simulans_ larvae and five _D. simulans_ and one _D. suzukii_ larvae at each collection, respectively, serving as a threshold for identification of _D. suzukii_ in the samples. Figure 1: Percentage of _Drosophila suzukii_ (SWD) flies emerged per sample collected from the three major strawberry production areas in California in 2019 and 2020. The symbol \({}^{\prime}\) for a site name indicates a second, later collection from the same site.