## 4 Discussion
The protein values were obtained with a nitrogen-to-protein conversion factor of 6.25, though [PERSON] et al. [37] suggested a conversion factor of 4.76 for the determination of protein in yellow mealworm larvae. Using this factor, the protein value was 49.00 g/100 g DM at 22 \({}^{\circ}\)C, 43.34 g/100 g DM at 25 \({}^{\circ}\)C, and 41.44 g/100 g DM at 28 \({}^{\circ}\)C. However, the effect of temperature on the nutritional value of _T. molitor_ resulting in a decrease in protein content with the increase in temperature remains confirmed. This tendency is in line with the literature [32; 33; 35; 36], where the values decreased from 57.8 \(\pm\) 2.1 to 37.9 \(\pm\) 1.4 g/100 g DM with the increase in temperature from 15 to 31 \({}^{\circ}\)C. The monitored decrease in the protein content with increasing temperature also agreed with [PERSON] et al. [10], who analysed _T. molitor_ when reared at seven different temperatures and observed a decrease in the protein content until 37 \({}^{\circ}\)C and a subsequent increase. At 39 \({}^{\circ}\)C, the protein content increased to 65.5 g/100 g DM. [PERSON] et al. [10] explained the different rates of larval development at different life stages and temperatures. At lower temperatures, the rate of development decreased because of slower metabolic reactions [38]. This might explain the differences in the protein or lipid contents [10]. Insects are considered to be an alternative protein source, and it was, therefore, appropriate to determine their amino acid profiles. The tryptophan content was not determined because this amino acid was degraded during acid hydrolysis. [PERSON] [39] reported a tryptophan content of 0.39 g/100 g DM for _T. molitor_ larvae. Based on a comparison of the results obtained with the amino acid requirements issued by the World Health Organization (WHO), the insects met the requirements with respect to the essential amino acid content and can, thus, be considered to be a good source of protein. The total amino acid content measured in this study (33.80 \(\pm\) 1.01 g/100 g DM at 22 \({}^{\circ}\)C, 36.28 \(\pm\) 0.95 g/100 g DM at 25 \({}^{\circ}\)C, and 31.02 \(\pm\) 0.84 g/100 g DM at 28 \({}^{\circ}\)C) was higher than that reported by [PERSON] et al. (29.83 g/100 g DM) [40]. Higher values were reported by [PERSON] et al. (44.50 g/100 g DM) [15]. [PERSON] et al. [15] reported that the larvae of _Allomyrina dichotomous_ or _Protaeta Brevitarsis_ had a higher amino acid content (48.74 and 39.16 g/100 g DM) than that of the investigated mealworms. A higher amino acid content compared with _T. molitor_ was also detected in egg white, which does not contain lipids or chitin (90.15 g/100 g DM), and chicken breast (88.25 g/100 g DM) [41]. In this study, the mealworm larvae contained the largest amounts of alanine at 22 and 25 \({}^{\circ}\)C and the largest amounts of proline at 28 \({}^{\circ}\)C. [PERSON] and [PERSON] [41], [PERSON] et al. [42], [PERSON] et al. [15], and [PERSON] et al. [40] reported that the glutamic acid content was the highest, followed by the alanine, aspartic acid, and proline contents. However, higher values of glutamic and aspartic acids were measured at all temperatures in this study. Sulphur amino acids (cysteine + methionine) and histidine were determined to be the least abundant amino acids in mealworms, which was consistent with the results reported by [PERSON] [39], [PERSON] and [PERSON] [41], [PERSON] et al. [42], and [PERSON] et al. [18]. With respect to the rearing temperature, no statistically significant differences were observed in aspartic acid, threonine, glycine, valine, leucine, phenylalanine, histidine, and methionine depending on the temperature. There were found significant differences in serine, glutamic acid, proline, isoleucine, tyrosine, and arginine between the temperatures of 25 and 28 \({}^{\circ}\)C, in alanine between 22 and 28 \({}^{\circ}\)C and between 25 and 28 \({}^{\circ}\)C, and in lysine among all temperatures.