## 3 Results
### Occurrence of Virulence Genes
\begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{2}{*}{**Strains**} & \multicolumn{2}{c}{**Non-\(\beta\)-Lactams Resistance**} & \multirow{2}{*}{_bla_ and PMQR Genes**} & \multirow{2}{*}{_int_ Genes**} & \multirow{2}{*}{**PRT**} \\  & & **Phenotypes Detected** & & \\ \hline EC 1 & NAL, CIP, TET, FOS, SXT & \(\textit{bla}_{\textit{CTX-M-15}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\) & - & F \\ \({}^{*}\)Tc EC 1 & TET & \(\textit{bla}_{\textit{CTX-M-15}}\) & - & F \\ \hline EC 7 & NAL, CIP, FOS & \(\textit{bla}_{\textit{CTX-M-15}}\), \(\textit{qnrS1}\) & - & - \\ Tc EC 7 & - & \(\textit{bla}_{\textit{CTX-M-15}}\) & - & - \\ \hline EC 8 & TOB, NAL, CIP, TET, MNO, FOS, & \(\textit{bla}_{\textit{CMV-2}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{bla}_{\textit{SHV}}\), \(\textit{qnrS1}\), & \(\textit{int2}\) & FIA-F \\ Tc EC 8 & CHL, SXT & \(\textit{a}_{\textit{acc}}\)(\({}^{\prime}\))ID-\(\sigma\) & \(\textit{int2}\) & FIA-F \\ Tc EC 8 & TET, MNO & \(\textit{bla}_{\textit{CMV-42}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\) & - & FIA \\ \hline EC 11 & GMN, TOB, NAL, CIP, TET, FOS, SXT & \(\textit{bla}_{\textit{CTX-M-15}}\), \(\textit{bla}_{\textit{SHV}}\), \(\textit{qnrS1}\) & - & F \\ Tc EC 11 & TET & \(\textit{bla}_{\textit{CTX-M-15}}\) & - & F \\ \hline EC 12 & NAL, FOS, SXT & \(\textit{bla}_{\textit{CTX-M-15}}\), \(\textit{qnrS1}\), \(\textit{aac}(^{\prime}\))ID-\(\sigma\) & \(\textit{int1}\) & FIA-F \\ Tc EC 12 & - & \(\textit{bla}_{\textit{CTX-M-15}}\) & \(\textit{int1}\) & FIA \\ \hline EC 19 & NAL, CIP, FOS & \(\textit{bla}_{\textit{CTX-M-15}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\) & - & FIA \\ Tc EC 19 & - & \(\textit{bla}_{\textit{CTX-M-15}}\) & - & FIA \\ \hline EC 20 & NAL, CIP, FOS, SXT & \(\textit{bla}_{\textit{CTX-M-27}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\) & - & - \\ Tc EC 20 & SXT & \(\textit{bla}_{\textit{CTX-M-27}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\) & - & - \\ \hline EC 21 & NAL, CIP, TET, MNO, FOS, SXT & \(\textit{bla}_{\textit{CTX-M-15}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\) & - & - \\ Tc EC 21 & TET, MNO, SXT & \(\textit{bla}_{\textit{CTX-M-15}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\) & - & - \\ \hline EC 24 & TOB, NAL, CIP, FOS, CHL, SXT & \(\textit{bla}_{\textit{CTX-M-15}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\), \(\textit{aac}(^{\prime}\))ID-\(\sigma\) & - & FIA-F \\ Tc EC 24 & TOB, CHL, SXT & \(\textit{bla}_{\textit{CTX-M-15}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\), \(\textit{aac}(^{\prime}\))ID-\(\sigma\) & - & FIA-F \\ \hline EC 27 & GMN, TOB, NAL, CIP, TET, FOS, & \(\textit{bla}_{\textit{CTX-M-15}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\), \(\textit{aac}(^{\prime}\))ID-\(\sigma\) & \(\textit{int1}\) & FIA-F \\ Tc EC 27 & TET & \(\textit{bla}_{\textit{CTX-M-15}}\) & \(\textit{int1}\) & F \\ \hline EC 28 & GMN, TOB, NAL, CIP, TET, FOS, & \(\textit{bla}_{\textit{CTX-M-15}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\), \(\textit{aac}(^{\prime}\))ID-\(\sigma\) & \(\textit{int1}\) & FIA-F \\ Tc EC 28 & TET, SXT & \(\textit{bla}_{\textit{CTX-M-15}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\) & \(\textit{int1}\) & FIA-F \\ Tc EC 29 & NAL, CIP, FOS, CHL, SXT & \(\textit{bla}_{\textit{CTX-M-9}}\), \(\textit{bla}_{\textit{CTX-M-27}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\), & - \\ Tc EC 29 & NAL, CIP, FOS, CHL, SXT & \(\textit{aac}(^{\prime}\))ID-\(\sigma\) & - & - \\ Tc EC 29 & CHL, SXT & \(\textit{bla}_{\textit{CTX-M-9}}\), \(\textit{bla}_{\textit{TEM}}\), \(\textit{qnrS1}\) & - & - \\ \hline \hline \end{tabular}  
* \({}^{*}\)Tc: transconjugant; -: negative character; NAL: naldixic acid; CIP: ciprofloxacin; SXT: trimethoprim-sulfamethoxazole; TET: tetracycline; MNO: minocyclin; GMN: gentamicin; TOB: tobramycin; FOS: fosfomic; CHL: chloramphenicol; PMQR: plasmid-mediated quinolone resistance; PRT: plasmid replicon type.  
\end{table} 
Table 5: Positive transconjugants compared with donor strains.