## 3 Results and Discussion
### Chemical Profile of Jubrication Wine Extracts  
The chromatograms obtained by LC-HRMS/MS analysis in the negative ionization mode showed JWE as a complex sample, highlighting the signals at 9-16 min and 25-42 min RT (Figure S4). JWF1 showed greater abundance and more intense signals between  
Figure 3: Effect of JWE and fractions (JWF2 and JWF3) on the total cellular antioxidant power of RBC. RBC were treated with maximal protective concentrations of JWF2 (7.8 \(\mathrm{\SIUnitSymbolMicro m}\)-\({}^{1}\)) and JWF3 (31 \(\mathrm{\SIUnitSymbolMicro m}\)-\({}^{1}\)) and challenged with 7 mM AAPH (OC\({}_{50}\)) for 4 h. Each point represents the mean of two independent experiments \(\pm\) standard error. The asterisk (*) indicates a significant difference between JWF2 and CNT (+) (ANOVA and Dunn’s test, \(p<0.05\)). CNT (-), negative control (untreated RBC); CNT (+), positive control (RBC treated with 7 mM AAPH). Figure 2: Effect of JWE and fractions (JWF1-3) on hematoprotection of RBCs against oxidative stress. RBCs were treated with several concentrations (\(\mathrm{\SIUnitSymbolMicro m}\)-\({}^{1}\)) of the extracts and challenged with 7 mM AAPH (OC\({}_{50}\)) for 4 h. Hematoprotective (antioxidant) effect of (**a**) JWE; (**b**) JWF1; (**c**) JWF2; and (**d**) JWF3. Ascorbic acid (AA) at 6 \(\mathrm{\SIUnitSymbolMicro m}\) was used as a reference antioxidant agent. Each point represents the mean of two independent experiments \(\pm\) standard error. 25-43 min RT, which is a region where more polar compounds are not typically found. Relevant signals between 24-38 min RT displaying \(m/z\) consistent with a flavonoid skeleton type were detected in JWF2. The chromatogram profile of JWF3 showed relevant signals in the intervals of 12-14 min and 28-38 min RT. The RT range is consistent with that of phenolic acids, which were tentatively identified in this study (Table 3). Regarding JWR, as expected, the chromatogram profile was similar to that of JWE, but with lower abundance and less intense ions. The results obtained in the positive ionization mode did not allow for verifying the efficiency of the partitioning process, as the chromatograms showed similar distribution, abundance, and ionic intensity (Figure S5). In addition, some expected compounds, such as pelargonidin or its glycosylated form could not be annotated. The ion intensity in the LC-HRMS/MS analysis was not relevant. The signals had a low count and were mixed with the analysis noise. Thus, there was no automatic fragmentation of the ions, preventing the analysis of the exact mass and fragmentation profile to confirm the presence of these compounds. After the LC-HRMS/MS analysis, 17 compounds were annotated, including carbohydrates, flavonoids, and organic acids (Table 2). These results agree with those previously reported for jabuticaba fruits [65; 66]. Here, we demonstrated that JWF2 and JWF3 stood out due to their antioxidant properties, including their hematoprotective activity. However, the LC-HRMS/MS analysis showed minor amounts of relevant antioxidant compounds (quercetin, isoquercitrin, cyanidin, and gallic acid) when compared to the other extracts. The partitioning process probably