## 4 Conclusions  
In this study, the water-soluble low-molecule WPI was prepared by hydrolyzing the WPI Superparamagnetic nanoparticles (\(\gamma\)-Fe\({}_{2}\)O\({}_{3}\), Fe\({}_{3}\)O\({}_{4}\)) prepared by hydrothermal methods were used as tumor-targeting carriers and combined with WPI into bio-modified superparamagnetic nanoparticles, which have an average diameter of 20 to 100 nm, crystal particle morphology, good stability, and biocompatibility under various conditions and were successfully loaded with an ideal load of Adriamycin. Due to the biological properties of the WPI, the efficacy of modified nanoparticles in magnetic hyperthermia was improved. Various chemical and physical analyses were conducted to verify the structure, morphology, biocompatibility, magnetic properties, and induction heating properties of the newly developed nanoparticles. In vitro and in vivo magnetic hyperthermia studies were conducted using HepG2 tumor and HepG2 mouse models to evaluate its clinical feasibility as a magnetic hyperthermia agent. In summary, the research findings fully prove that WPI-modified superparamagnetic nanoparticles have excellent AC induction heating power and biocompatibility in the biologically safe range of AC magnetic field usage. Thus, it should be further popularized as a new magnetic hyperthermia agent for treating tumors.  
Conceptualization, S.Z.; Data curation, S.Z.; Formal analysis, S.Z.; Funding acquisition, S.L.; Investigation, S.Z.; Project administration, S.L.; Validation, S.Z.; Visualization, S.L.; Writing--original draft, S.Z.; Writing--review & editing, S.L. All authors have read and agreed to the published version of the manuscript.  
This research was financially supported by the Brain Korea 21 Plus (BK21+) Project of the GNU Graduate Education and Research Program for Mechanical and Aerospace Engineers.  
Figure 7: (**a**) Model of in vivo magnetic hyperthermia with mice carrying HepG2 tumors; (**b**) results of tumor temperature during in vivo magnetic hyperthermia; (**c**) model of high-energy thermal inductance (HETI) equipment for check result of tumor scale after in vivo magnetic hyperthermia; (**d**) set result of tumor scale: the first row using in vivo magnetic hyperthermia with WPI-Fe\({}_{2}\)O\({}_{3(85 wt^{\prime})_{3}}\). The middle row using treatment with Adriamycin. The bottom row using treatment with normal saline; (**e**) the survival curve of mice at 0 day after magnetic hyperthermia; (**f**) the survival curve of mice at 7 days after magnetic hyperthermia.  
**Institutional Review Board Statement:** The study was conducted according to the guidelines of the Declaration of Helsinki, and approved by the Gyeongsang National University Institutional Animal Care Use Committee (GNU2016-121).  
**Informed Consent Statement:** Not applicable.  
**Data Availability Statement:** Not applicable.  
**Acknowledgments:** We are grateful to Nano-Information Materials Laboratory of the Department of Materials Engineering and Polymerization Technology of Gyeongsang National University for providing resources and support to complete this research. We are also grateful to the Food Microbiology Laboratory and the Animal Neuroscience Laboratory of Gyeongsang National University for their guidance and help in research.  
**Conflicts of Interest:** The authors declare no conflict of interest.