## 3 Results  
### Statistical Analysis  
Table 1 shows the mean values and standard deviations and 25 th and 75 th percentiles for all variables collected in the database. This result was obtained using pandas and numpy library in Phyton 3.9. Analyzing the statistical results, we can describe the overall database used in this study. The mean age of the subjects is 60.9 (11.47 std) and they have a mean BMI of 31.73 (6.15 std), indicating an obesity-prone group. The mean ESC values for hands and feet place the group in the one without the risk of neuropathy. However, it should be taken into account that patients with neurotrophic treatment (21% of all subjects) were included in these data. \begin{table}
\begin{tabular}{c c c c} \hline \hline  & **Mean (std)** & **25 th Percentile** & **75 th Percentile** \\ \hline Age & 60.9 (11.47) & 55.0 & 69.0 \\ BMI & 31.73 (6.15) & 27.5 & 35.0 \\ Left foot & 62.8 (15.36) & 50.0 & 75.0 \\ Right foot & 61.4 (15.78) & 49.0 & 74.0 \\ Left hand & 57.5 (14.73) & 48.0 & 69.0 \\ Right hand & 57.8 (14.38) & 49.0 & 69.0 \\ Neuro risk & 60.0 (13.19) & 50.2 & 71.3 \\ Cardio risk & 37.3 (11.37) & 30.0 & 43.0 \\ Neftro risk & 61.5 (18.65) & 49.0 & 71.0 \\ DM age & 11.02 (8.63) & 4.0 & 16.0 \\ HbA1C & 8.73 (2.00) & 7.2 & 9.9 \\ Cholesterol & 202.9 (48.76) & 172.7 & 233.3 \\ Triglyceride & 159.0 (81.2) & 103.0 & 185.0 \\ SBP & 143.0 (20.13) & 129.0 & 156.3 \\ DBP & 80.0 (12.06) & 70.0 & 87.3 \\ Creatinine & 0.9 (0.25) & 0.6 & 1.0 \\ Percentage of Type 1 & 12.21\% & \\ Diabetes & & & \\ \hline BMI—Body Mass Index; DM age—the age of Diabetes Mellitus; HbA1C—glycated hemoglobin; SBP—Systolic Blood Pressure; DBP—Dastolic Blood Pressure. & & \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Characteristics of the data collected from the 172 subjects. The mean age at diabetes diagnosis is 11.02 years, suggesting that patients were diagnosed with diabetes around the age of 50. Glycated hemoglobin (HbAlc) is an indicator of long-term glycemic control and has a mean of 8.73%, indicating poor glycemic control in this group of subjects. The mean values for SBP and DBP show a tendency of this group towards hypertension. The mean values for cholesterol and triglyceride classify the group as hypercholesterolemia and hypertriglyceridemic, and the standard deviation values show the high variability of these biochemical values among the target group. In Figure 1 the boxplots represent the Electrochemical Skin Conductance (ESC) distribution for left hand/leg and right hand/leg, grouped according to diabetic autonomic neuropathy (DAN) status: possible DAN, confirmed DAN, and no DAN. In Table 2, an analysis of the ESC results obtained by patients in both hands and feet according to BMI and separated for those on and off neurotrophic treatment was performed. The Interpretation column represents the BMI coding as follows: 0--normal weight, 1--overweight, and 2--obese. Interpretation of ESC results is as follows: ESC values > 60 uS indicate DAN, ESC values between 40 and 60 uS indicate possible DAN for hands and between 40 and 50 for feet; ESC values < 40 for hands and ESC < 50 for feet indicate NO DAN. Thus, it can be seen that there is a higher incidence of DAN or possible DAN for obese or overweight patients, which is in agreement with the literature [22] which states that a high weight status leads to early complications in patients with diabetes. This tendency to overweight and obesity is present in 61% of all patients in this study. Neurotrophic treatment clearly improves SUDOSCAN values. Figure 1: Distribution of ESC values for hands and feet grouped by DAN. Table 3 represents an analysis of the ESC results obtained by patients in both hands and feet according to neurotrophic treatment (yes--1 or no--0) and total cholesterol values. Cholesterol values were divided into three groups: 0--values < 180 mg/dl, 1--values between 180 and 200 mg/dl, and 2--values greater than 200 mg/dl. In the no-treatment group, most patients with DAN are in category 2 (total cholesterol values: 200 mg/dl).