## 3 Results and Discussion
### Determination of Macro- and Microminerals  
The contents of various minerals (N, Mg, Ca, S, Na, Al, B, Cu, Fe, Mn, Zn, Mo, Ni, Ag, As, Cd, Pb, Sb and Tl) in potato tubers biofortified with each treatment are presented in Table 3. The study shows that the contents of the macrominerals N, K, Ca, S, Na, and the trace elements As and Cd, in potato tubers biofortified with KIO\({}_{3}\), 8-OH-7-I-5 QSA acid and 5-Cl-7-I-8-Q did not change compared to the control. For phosphorus (P) and magnesium (Mg), biofortification with 8-OH-7-I-5 QSA decreased the content of these elements in potato tubers in a relatively small but statistically significant way. This is not beneficial, as these elements have a positive effect on the functioning of the bones (P) and the muscular and nervous systems (Mg) [2]. Most of the daily requirements of these elements are covered by potato consumption. Biofortification reduced the aluminium (Al) accumulation in potato tubers enriched with KIO\({}_{3}\), 8-OH-7-I-5 QSA, 5-Cl-7-I-8-Q. Aluminium, like other metals, can act as a substitute for magnesium, calcium or iron ions, and thereby block the action of enzymes that are dependent on them. This results in changes in the synthesis of essential proteins, in the function of genetic material and the permeability of cell membranes [2]. Its excess in the diet is not beneficial, and thus a reduction in the aluminium content after biofortification is a good effect. When compared to the control, the boron (B) content did not change in a statistically significant way after the application of all the tested iodine compounds. Boron protects against excessive fluoride accumulation and aids in its removal, facilitates vitamin D absorption and is responsible for maintaining adequate calcium levels in the human body [2]. It was observed that the application of KIO\({}_{3}\) had a statistically significant effect on the increase in copper (Cu) and manganese (Mn) content. However, compared to the control, the application of 8-OH-7-I-5 QSA and 5-Cl-7-I-8-Q did not affect the concentrations of Cu and Mn in potato tubers. Copper in the body performs the role in maintaining normal levels of collagen and elastin, which are the main structural components of our body. Manganese,on the other hand, regulates bone metabolism and promotes bone growth and healing, which is particularly important during post-traumatic recovery and in the prevention of osteoporosis [2]. Their higher accumulation in the potato tuber is a positive consequence of biofortification. This study shows that only biofortification using 5-Cl-7-I-8-Q had a statistically significant effect on the reduction in the iron (Fe) content of tubers. However, in comparison with the control, in each treatment that applied iodine compounds, there was a decrease in the iron content of the tubers. Some varieties of _Solanum tuberosum_ L. contain iron in amounts that are comparable to those found in some cereals (rice, maize, wheat). The iron of _Solanum tuberosum_ L. should be bioavailable because, unlike cereals, it contains very little phytic acid [18]. The zinc (Zn) content of the tubers that were enriched only with KIO\({}_{3}\) was statistically significantly higher than that in the control. _Solanum tuberosum_ L. of different potato varieties contains zinc in the amount of 0.5-4.6 \(\mathrm{\SIUnitSymbolMicro  
Biofortification with KIO\({}_{3}\) acid significantly increased the cobalt (Co) content of potato tubers, in contrast to organic forms of enrichment with 8-OH-7-1-5 QSA and 5-Cl-7-I-8-Q, where no statistically significant changes in the cobalt content were shown in potato tubers. Cobalt has a regulatory function in the production of red blood cells, as well as in the metabolism of nucleic acids and proteins. It is also an essential element for pregnant women, as it is involved in the production of vitamin B9 (folic acid) [2].