## 3 Results
### Effects of TJ on Cell Viability in 3T3-L1 Cells
NC, normal control; DC, differentiated control; PC1, catechin 100 \(\mathrm{\SIUnitSymbolMicro g}\)/mL; PC2, catechin 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL; TJ (10, 50, 100, or 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL). The data are shown as mean \(\pm\) standard deviation (SD; n = 3), and statistical analyses were conducted by [PERSON]’s multiple range tests after one-way ANOVA. Each lowercase alphabet indicates within-figure differing significance (\(p<\) 0.05). The TJ Regulated the Protein Expression Levels Related to the Lipogenesis Mechanism in Differentiated 3T3-L1 Cells  
Upon measuring the protein expression level related to the mechanism of suppressing lipogenesis, the G6 PDH/actin ratio was significantly reduced in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (1.01 \(\pm\) 0.10, 0.84 \(\pm\) 0.04, 0.58 \(\pm\) 0.05, and 0.41 \(\pm\) 0.04, respectively) in a dose-dependent manner relative to that of the DC group (1.10 \(\pm\) 0.05) (Figure 5A,B). The citrate synthase/actin ratio was significantly reduced in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (1.02 \(\pm\) 0.12, 0.78 \(\pm\) 0.02, 0.47 \(\pm\) 0.10, and 0.30 \(\pm\) 0.02, respectively) in a dose-dependent manner relative to that of the DC group (1.50 \(\pm\) 0.05) (Figure 5A,C). The phospho-AMPK/AMPK ratio was significantly increased in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (0.54 \(\pm\) 0.07, 0.99 \(\pm\) 0.01, 1.13 \(\pm\) 0.10 and 1.47 \(\pm\) 0.05, respectively) in a dose-dependent manner relative to that of the DC group (0.20 \(\pm\) 0.05) (Figure 5A,D). The SREBP1/actin ratio was significantly reduced in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (0.86 \(\pm\) 0.03, 0.91 \(\pm\) 0.04, 0.76 \(\pm\) 0.10, and 0.42 \(\pm\) 0.05, respectively) in a dose-dependent manner relative to that of the DC group (0.87 \(\pm\) 0.05) (Figure 5A,E). The phospho-ACC/ACC ratio was significantly increased in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (0.46 \(\pm\) 0.17, 1.00 \(\pm\) 0.10, 1.07 \(\pm\) 0.09, and 2.08 \(\pm\) 0.15, respectively) in a dose-dependent manner relative to that of the DC group (0.39 \(\pm\) 0.15) (Figure 5A,F). The FAS/actin ratio was significantly reduced in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (1.22 \(\pm\) 0.05, 0.89 \(\pm\) 0.05, 0.64 \(\pm\) 0.05, and 0.67 \(\pm\) 0.13, respectively) in a dose-dependent manner relative to that of the DC group (1.48 \(\pm\) 0.05) (Figure 5A,G). The LPL/actin ratio was significantly reduced in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (0.91 \(\pm\) 0.08, 0.88 \(\pm\) 0.05, 0.72 \(\pm\) 0.06, and 0.58 \(\pm\) 0.14, respectively) in a dose-dependent manner relative to that of the DC group (1.56 \(\pm\) 0.05) (Figure 5A,H). Figure 5: Effects of the TJ on lipogenesis-related proteins in 3T3-L1 cells: (**A**) Western blot showing the expression levels of the proteins, (**B**) G6 PDH/actin, (**C**) citrate synthase/actin, (**D**) p-AMPK/AMPK, (**E**) SREBP1/actin, (**F**) p-ACC/ACC, (**G**) FAS/actin, and (**H**) LPL/actin. NC, normal control; DC, differentiated control; PC1, catechin 100 \(\mathrm{\SIUnitSymbolMicro g}\)/mL; PC2, catechin 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL; TJ (10, 50, 100, or 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL). The data are shown as mean \(\pm\) standard deviation (SD; n = 3), and statistical analyses were conducted by [PERSON]’s multiple range tests after one-way ANOVA. Each lowercase alphabet indicates within-figure differing significance (\(p<\) 0.05). The Effects of the TJ in the cAMP Level, Intracellular FA, TG, and Glycerol Release Related to the Lipolysis Mechanism in Differentiated 3T3-L1 Cells  
As a result of measuring the level of cAMP acting on the process of lipolysis in differentiated 3T3-L1 cells, we confirmed that in the DC group (701.7 \(\pm\) 10.0 pmol/mL), were significant differences compared to that of the NC group (1071.7 \(\pm\) 60.4 pmol/mL).