## 3 Results
### Chamelea Gallina  
No mortality occurred in any of the treatments after 21 days of exposure, so survival was 100%. The results of NRT and %LMS values of _C. gallina_ are presented in Figure 2 and Table S1. There was a significant difference in LMS (expressed as NRT) as a result of temperature and the interaction of both factors (temperature and salinity) but no effect of salinity (2-way ANOVA; \(p\)-value < 0.05) (Table 2). LMS in _C. gallina_ hemocytes (expressed \begin{table}
\begin{tabular}{c c c c c c c c c c} \hline \hline \multicolumn{2}{c}{**Test Treatment**} & \multicolumn{6}{c}{_Chameka gallina_} \\ \hline  & **T** & **S** & **T** & **S** & **DO** & **DO** & **T** & **S** & **DO** & **DO** \\  & **T** & **S** & **(°C)** & **(mg/L)** & **(\% sat.)** & **(°C)** & **(°C)** & **(mg/L)** & **(\% sat.)** \\ \hline
1 & & 27–28 & 12.30 \(\pm\) 0.05 & 27.09 \(\pm\) 0.06 & 9.06 \(\pm\) 0.01 & 104.79 \(\pm\) 1.41 & 12.13 \(\pm\) 0.03 & 27.32 \(\pm\) 0.04 & 9.12 \(\pm\) 0.01 & 106.01 \(\pm\) 0.09 \\
2 & Low & 32–33 & 12.00 \(\pm\) 0.03 & 32.56 \(\pm\) 0.14 & 9.12 \(\pm\) 0.01 & 104.75 \(\pm\) 1.41 & 12.02 \(\pm\) 0.04 & 32.72 \(\pm\) 0.08 & 9.14 \(\pm\) 0.01 & 106.10 \(\pm\) 0.11 \\
3 & & 37–38 & 12.17 \(\pm\) 0.03 & 38.12 \(\pm\) 0.05 & 9.09 \(\pm\) 0.02 & 103.26 \(\pm\) 0.98 & 12.26 \(\pm\) 0.04 & 37.93 \(\pm\) 0.04 & 9.10 \(\pm\) 0.01 & 106.19 \(\pm\) 0.15 \\ \hline
4 & & 27–28 & 20.11 \(\pm\) 0.05 & 27.35 \(\pm\) 0.03 & 7.79 \(\pm\) 0.01 & 105.82 \(\pm\) 0.08 & 20.23 \(\pm\) 0.01 & 27.33 \(\pm\) 0.01 & 7.76 \(\pm\) 0.00 & 105.64 \(\pm\) 0.24 \\
5 & Medium & 32–33 & 19.95 \(\pm\) 0.04 & 32.70 \(\pm\) 0.04 & 7.64 \(\pm\) 0.04 & 102.38 \(\pm\) 0.06 & 20.16 \(\pm\) 0.01 & 32.78 \(\pm\) 0.01 & 7.77 \(\pm\) 0.00 & 103.49 \(\pm\) 0.73 \\
6 & & 37–38 & 20.34 \(\pm\) 0.05 & 37.63 \(\pm\) 0.06 & 7.72 \(\pm\) 0.02 & 102.29 \(\pm\) 0.87 & 20.07 \(\pm\) 0.01 & 37.47 \(\pm\) 0.01 & 7.78 \(\pm\) 0.00 & 105.51 \(\pm\) 0.40 \\
7 & & 27–28 & 27.13 \(\pm\) 0.10 & 27.95 \(\pm\) 0.05 & 6.91 \(\pm\) 0.01 & 104.77 \(\pm\) 0.25 & 27.58 \(\pm\) 0.12 & 27.55 \(\pm\) 0.18 & 6.79 \(\pm\) 0.02 & 105.96 \(\pm\) 0.09 \\
8 & High & 32–33 & 26.94 \(\pm\) 0.09 & 32.73 \(\pm\) 0.09 & 6.91 \(\pm\) 0.01 & 105.70 \(\pm\) 0.06 & 27.40 \(\pm\) 0.12 & 32.57 \(\pm\) 0.07 & 6.86 \(\pm\) 0.01 & 105.97 \(\pm\) 0.13 \\
9 & & 37–38 & 26.98 \(\pm\) 0.11 & 37.71 \(\pm\) 0.10 & 6.93 \(\pm\) 0.01 & 105.03 \(\pm\) 0.13 & 27.44 \(\pm\) 0.15 & 37.57 \(\pm\) 0.08 & 6.82 \(\pm\) 0.02 & 105.95 \(\pm\) 0.10 \\ \hline \end{tabular}
\end{table} 
Table 1: Seawater parameters (mean \(\pm\) SE) during the 21 days experiment conducted with _Donax trunculus_ and _Chamelea gallina_. N: treatment number; T: temperature; S: salinity; DO: dissolved oxygen (mg/L and % oxygen saturation). as mean value of NRRT) was higher in organisms from the 20 \({}^{\circ}\)C/27-28, 20 \({}^{\circ}\)C/37-38, and 12 \({}^{\circ}\)C/32-33 salinity treatments (Figure 2a), where hemocytes showed a longer retention time of neutral red within the lysosomal compartment (Figure 3A). Pairwise comparisons revealed three homogeneous subsets among the nine combined treatments (Tukey-b test; \(p\)-value \(<\) 0.05) (Table S2). At a low temperature (12 \({}^{\circ}\)C), NRRT was significantly higher in animals exposed to salinity of 32-33, compared to those maintained at 27-28 and 37-38 (\(p\)-value \(=\) 0.003) (Figure 2a and Table S3). However, an opposite pattern was found at a medium temperature (20 \({}^{\circ}\)C), with NRRTs being higher at salinities 27-28 (\(p\)-value \(=\) 0.002). When LMS was estimated by adding information of lysosomal alterations to the NRRTs (%LMS), no differences were observed due to temperature and salinity, but there was a significant interaction between these factors (2-way ANOVA; \(p\)-value \(\leq\) 0.001) (Table 2).