## 4 Discussion
### Secretion of Enzymes Related to Degradation of Lignocellulose  
The degradation efficiency of the wood substrates is determined by the action of fungal enzyme systems and depends on their qualitative and quantitative composition. The different types of lignocellulosic destruction patterns observed in fungi are mediated by the presence in their genomes of gene families encoding carbohydrate-active enzymes (CAZymes), mainly glycosyl hydrolases (GH) and the auxiliary activity (AA) family [12]. In this regard, the _C. laeye_ genome is similar to that of the WRF _Trametaceae_ family, with a complete set of CAZyme families associated with the degradation of all plant cell wall components [29]. A unique difference is the presence of a large number of gene copies--52, 34, 15 and 10 versus (15-21), (15-18), (6-7) and (2-3) belonging under AA1_1, AA3_2, AA7 and AA9 CAZyme families in the _Trametes_ sp. Genomes, respectively [29]. It is known that the production of WRF enzymes is determined both by their taxonomic position and ecological niche [47]. Xylotrophic WRF of the order Polyporales, in particular representatives of the genera _Polyporus_[11], _Pycaporus_[10; 48; 49], _Dichomitus_[50] and _Trametes_[51], when cultivated on media with sawdust, secrete a significant amount of typical cellulolases of the GH5, GH6 and GH7 CAZy families and hemicellulases of the GH2, GH3, GH5, GH10, GH11 and GH28 CAZy families, as well as cellulose degrading enzymes such as lytic polysaccharide monooxygenases (LPMOs) and cellobiose dehydrogenase (AA3). For lignin degradation, WRF secrete various ligninolytic enzymes (LDEs), including lacases (AA1) and class II peroxididases (PODs, AA2) [52; 53]. However, different Polyporales species exhibit different patterns of LDE secretion: (i) secretion of Lac and PODs (MnP, LiP and VP)--_Trametes versicolor_; (ii) secretion of Lac and one of the PODs (MnP, or LiP, or VP)--_Ceriporiopsis subvernispora_; and (iii) secreting only PODs--_Phareochaete chrysosporium_[54]. WRF of the order Agarcales, in particular the genera _Schizophyllum_, _Agaricus_ and _Cyclotype_ (=_Agrocybe_), whose members are litter-degrading or humus (soil) saprotrophs when grown on media with lignocellulosic substrates, also secrete cellulases and hemicellulases, but a wider range of hemicellulases hydrolyze the side chains of polysaccharides (family GH27, GH31, GH35, GH43, GH51, GH115, CE1, CE15 CAZy). However, unlike Polyporales, the secretomes of WRF Agarcales lack PODs, while some species secrete lacases (_Agaricus bisporus_), and others do not (_Schizophyllum commune_). Another distinguishing feature of the secretomes of WRF Agarcales from Polyporales is the high diversity of LPMOs, glucooligosaccharide oxidases (AA7 CAZy) and expansinelike proteins [55; 56; 57]. Some WRF Agarcales species show less cellulose degradation and more lignin degradation, while others in contrast show more intense cellulose degradation. Therefore, some species of this taxonomic group were not susceptible to the WR/BR classification and occupy an intermediate position between white and brown rot fungi--_S. commune_ and _Armillaria_ spp. [58; 59]--or between white and soft rot fungi, as in the case of _Armillaria ostoyae_ and _Armillaria cepisites_[60], and the species _Mucidula mucida_ (= _Oudemansiella_) is capable of causing both soft and white rot depending on the stage of decomposition and the type of wood substrate [59; 61]. Similarly to other WRF of the order Agarcales, oxidative enzymes such as lytic polysaccharide monooxygenases (LPMOs), lacases and oxidoreductaseases, families AA3_2 and AA7 CAZymes and also expansin-like proteins were identified in the _C. laeev_ secretomes. Moreover, their secretion was increased manifold on the sawdust-containing media and additional isoenzymes appeared (Figure 4, Supplementary Figure S1). At the same time, _C. laeev_ secretomes lacked the major cellulose- and hemicellulose-degrading enzymes (GH1, GH2, GH3, GH5, GH10 and GH11 CAZy).