## 5 Materials and Methods
Data is sorted according to high, low, or medium quality, or not-determined based on matches to the CheckV database. Figure S5. Immune-related transcripts that are predicted from the annotation of the _A. domesticus_ assembly. Predicted transcripts include: PGRP--peptidoglycan-recognition protein; GNBP--b-1-3-glucan-binding protein; lysozyme; PPO--prophenyloxidase. Figure S6. Supporting data for the comparison of intron length, genome size, and repeat content in orthopteran species (six crickets, including _Acheta domesticus_, _Aptonenombius asalthai_, _Gryllus bimaculatus_, _Luapula kohalensis_, _Teleogryllus occipitalis_, _Teleogryllus oceanicus_ and one locust, _Locusta migratoria_). (A) LINE, (B) DNA, and (C) LTR transposon subclasses and correlation with intron length and genome size. Table S7. Comparison of intron length, genome size, and repeat content in orthopteran species (six crickets, including _Acheta domesticus_, _Apteronembius asahtinai_, _Gryllus bimaculatus_, _Laupala kohalensis_, _Telogryellus occipitalis_, _Telogryellus oceanicus_ and one locust, _Locusta migratoria_). (A) Correlation between total repeat content and genome size; (B) Correlation between total repeat content and median length of intron; (C) Correlation between genome size and median length of intron; and Comparison of (D) LTR, (E) LINE, and (F) DNA transposable elements. Table S8. CRISPR sgRNA target sequences for the Ad vermillion gene. Table S9. Double-stranded RNA sequences used for RNAi experiments. Figure S10. Name and annotation of predicted _A. domesticus_ genes, with scaffold number and length. Figure S11. Transcript sequences of predicted _A. domesticus_ genes. Figure S12. _A. domesticus_ egg microinjection slide. (A) Slide set up with eggs positioned on each side of the filter paper strips; (B) Injected eggs showing microinjection site with phenol red color in eggs; and (C) Developed eggs after microinjection showing eye pigmentation. [PERSON], F.-C.C., B.O., M.D.L., B.S., S.S., S.K. and J.S.J. performed research; [PERSON], F.-C.C., B.O., M.D.L., B.S., S.S., J.S.J., K.K. and K.I., analyzed data; and [PERSON], F.-C.C., B.O., M.D.L., B.S., S.S., J.S.J., K.K. and K.I., wrote the paper. All authors have read and agreed to the published version of the manuscript. This material is based upon work supported by the Defense Advanced Research Projects Agency (DARPA) under Contract No. 140D6318C0055. The research was completed under Cooperative Research and Development Agreement Number 58-3020-7-013 between ARS, ATB, and NCSU. This study was supported by the Cabinet Office, Government of Japan, Cross-ministerial Moonshot Agriculture, Forestry and Fisheries Research and Development Program, \"Technologies for Smart Bio-industry and Agriculture\" (BRAIN) [JPJ009237 to KK]. This work was supported, in part, by the Intramural Research Program of the National Human Genome Research Institute, National Institutes of Health (SK). This work utilized the computational resources of the NIH HPC Biowulf cluster ([[https://hpc.nih.gov](https://hpc.nih.gov)]([https://hpc.nih.gov](https://hpc.nih.gov))). This research was performed in accordance with Kansas State University Research Compliance Office, Institutional Biosafety Committee registration number 1191, \"Functional Genomics of Stored Product Insects\". Not applicable. All data was deposited at NCBI, including the _A. domesticus_ genome assembly--JAHLJ7010000000, transcriptome assembly--GHUU00000000, and metagenome scaffolds--PRJNA908265. We thank [PERSON] for bioinformatics support, and [PERSON] for analysis of _A. domesticus_ scaffolds in GX beta testing. [PERSON] also thanks [PERSON] (Ripple Technology, LLC, Atlanta, GA, USA) for his help and organizational support. This genome assembly is a contribution to the i5k and USDA ARS AgPest100 projects. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.