# Advances in Engineering and Application of Optogenetic Indicators for Neuroscience
## 2 Calcium Indicators
\begin{tabular}{|c|c|c|c|c|c|c|c|c|c|c|c|} \hline \multirow{2}{*}{**Parameter**} & \multirow{2}{*}{**Indicator** *} & \multirow{2}{*}{**Indicator** *} & \multirow{2}{*}{**Ex/Fin (mm)** *} & \multirow{2}{*}{**Bighness vs.** **ECFP (\%)-**} & \multirow{2}{*}{**Ex/F**} & \multirow{2}{*}{**Ex/F**} & \multirow{2}{*}{**Ex/F**} & \multirow{2}{*}{**Ex/F**} & \multirow{2}{*}{**Ex/F**} & \multirow{2}{*}{**Ex/F**} & \multirow{2}{*}{**Ex/F**} \\  & & & **(mm)** * & & & & & **(\%)** & **(\%)** & **(\%)** \\  & & & & & & & & **(\%)** & **(\%)** & **(\%)** \\ \hline \multicolumn{12}{|c|}{**Calcium indicators**} \\ \hline \multicolumn{12}{|c|}{**Calcium indicators**} \\ \hline \multicolumn{12}{|c|}{**NToC**} & 50\%518 & 163 & 6.09k0.18 & 100 & 102 & 0.8 & 0.02718 & ND & [28] \\ \cline{2-12} \multirow{12}{*}{**Parameter**} & 1Y**TrC** & 49\%518 & 16 & 7.48.5 & 480 & 331 & 1.12 & 0.0015/ND & ND & [41] \\ \cline{2-12}  & **YTrC** & 49\%516 & 17 & 5.26.3 & 290 & 410 & 0.96 & 0.008/ND & ND & [39] \\ \cline{2-12}  & **dY**TrC** & 49\%518 & ND & ND & 1900 & 2700 & ND & ND & ND & [unpublished] \\ \hline \multirow{12}{*}{**Parameter**} & ****\#po** & 402/516 & 56 & 6.56f/0.7 & 590 & 400 & & & & \\ \cline{2-12}  & **FGCAMP* & \multirow{2}{*}{sat} & 490/516 & 104 & 6.27.33 & 1370 & 440, & & & & \\ \cline{2-12}  & **GCAMP* & \multirow{2}{*}{497/515} & 124 & 9.77k/20 & 6220 & 227 & 0.69-1.12 & 0.287/6- & 0.258/ND & [26] \\ \cline{2-12}  & **GCAMP* & \multirow{2}{*}{497/515} & 109 & 8.77/6.34 & 5080 & 492 & 3.93 & 0.187/101 & 0.35/ND & [26] \\ \cline{2-12}  & **ICAMP* & \multirow{2}{*}{497/515} & 103 & 7.69k/36 & 3900 & 68* & 2.86 & 0.687/25 & ND & [37] \\ \cline{2-12}  & **jBCECO1a** & 560/590 & 35 & 8.6k/3.3 & 1000 & 148* & 7.6 & -0.289/-13 & 0.13/ND & [9,42] \\ \cline{2-12}  & **K-GECO1** & 560/590 & 82 & ND & 1100 & 165* & ND & -0.28/-11 & ND & [43] \\ \hline \multirow{12}{*}{**Parameter**} & \multirow{2}{*}{**COMPARIZED**} & \multirow{2}{*}{**C**} & \multirow{2}{*}{502/516} & \multirow{2}{*}{268} & \multirow{2}{*}{ND} & \multirow{2}{*}{**Ex/F**} & \multirow{2}{*}{**Ex/F**} & \multirow{2}{*}{**Ex/F**} & \multirow{2}{*}{**Ex/F**} & \multirow{2}{*}{**Ex/F**} & \multirow{2}{*}{**Ex/F**} \\  & & & & & & & & & & \\ \cline{2-12}  & **R** & 562/577 & 126 & ND & & & & & & \\ \hline \multirow{12}{*}{**Parameter**} & **GAF-cAMP** & 63\%5/672 & 21, 69* & 3.09/10.0 & 52 & 15 & ND & ND & ND & [44] \\ \cline{2-12}  & **NIB-GECO1** & 678/704 & ND & 6.04/7 & 88 & 215 & 1.93 & 2.5/25 & ND & [45] \\ \cline{2-12}  & **LOCL-GECO2** & \multirow{2}{*}{460/5515} & \multirow{2}{*}{ND} & \multirow{2}{*}{8.15k/0.7} & \multirow{2}{*}{406} & \multirow{2}{*}{285*} & \multirow{2}{*}{ND} & \multirow{2}{*}{ND} & \multirow{2}{*}{ND} & \multirow{2}{*}{[46]} \\ \cline{2-12}  & **LOCL-GECO1** & & & & & & & & \\ \cline{2-12}  & **acceptor** & \multirow{2}{*}{497/515} & \multirow{2}{*}{ND} & \multirow{2}{*}{ND} & \multirow{2}{*}{8.15k/0.7} & \multirow{2}{*}{406} & \multirow{2}{*}{285*} & \multirow{2}{*}{ND} & \multirow{2}{*}{ND} & \multirow{2}{*}{ND} & \multirow{2}{*}{[47]} \\ \cline{2-12}  & **Potassium indicators** & \multirow{2}{*}{**donor**} & 433/475 & 104 & 3.2 & & & & & \\ \cline{2-12}  & **KIRIN* & \multirow{2}{*}{**acceptor**} & 515/530 & ND & ND & & & & & \\ \cline{2-12}  & **KIRIN-L** & **donor** & 50\%5/515 & 251 & 6.2 & 20 & 2560 & ND & ND & ND & [47] \\ \cline{2-12}  & **GEMP* & \multirow{2}{*}{**acceptor**} & 50\%5/500 & 128 & 5.3 & & & & & & \\ \cline{2-12}  & **GEMP* & \multirow{2}{*}{**acceptor**} & 457/475 & ND & ND & ND & & & & & \\ \cline{2-12}  & **GINKO1** & 502/514 & 22 & ND & 250 & 420 & ND & ND & ND & [47] \\ \hline \end{tabular}   
* \({}^{\text{a}}\), \({}^{\text{b}}\), \({}^{\text{c}}\), \The original NTnC sensor was designed by inserting the troponin C calcium binding domain into the bright green fluorescent protein mNeonGreen [38]. In contrast to GCaMP-like sensors, NTnC exhibits negative fluorescence response, i.e., it reduces fluorescence upon calcium binding.