## 4 Discussion  
According to the morphological characteristics corresponding to the genus _Xanthomona_, it was confirmed that Xcf1-APJR strain is within this bacterial genus. [PERSON] et al. [27] indicated that _Xanthomona_, is represented by mocoid colonies, which are convex and bright in NGA. The growth of the isolate in YDC confirmed the presence of the yellow colonies of _Xanthomonas_. [28]  
The sequence of rDNA 16S gene region of the Xcf1-APJR strain showed 100% identity with _X. campestris_ pv. _campestris_ (ID: NZ_AP019684) [29,30]. Likewise, the serological method endorsed the identification of strain Xcf1-APJR as _X. campestris_ pv. _campestris_, [27]. The pathogenicity test revealed similar results to those already reported by [PERSON] et al. [28] for _X. campestris_. The typical symptoms emerged five days post-inoculation, and hypersensitivity was observed 24 h after inoculation. The wet necrotic spots surrounded by a chlorotic halo in the leaves showed the beginning of necrosis at the tissue level, which presented in the same incubation period reported by [PERSON] et al. [31]. The necrotic foliar stain with chlorotic halo, on the margins of the leaf, was also reported by [PERSON] et al. [32], who observed this symptom on mature leaves of _Passiflora edulis_ at five days post-inoculation. After the assessment of pathogenicity tests with the re-isolated Xcf2-APJR strain, Koch's confirmed this bacterium as the causal agent of bacterial blight. [PERSON] et al. [33] first reported on _X. campestris_ pv. _campestris_ as the causal agent of black rot in rapeseed (_Brassica napus_) in Serbia. As far as we know, this is the first report of this pathogen causing black leaf rot in _P. vulgaris_ in variety \"Flor de Mayo M38\" in Puebla, Mexico. The growth of the CP-MaPs strain was completed at day 7 in all the treatments. This result differed from that reported by [PERSON] et al. [34]. The morphology of the colonies coincided with that reported by [PERSON] [35] for _P. sanguineus_, which showed aerated and velvety orange mycelium at 29 days post-inoculation. [PERSON] et al. [34] reported growth rates of 5.6 and 7.14 mm/day for wild strains of _P. sanguineus_ (MIP20001 and MIP95002, respectively) isolated from Santa Catarina, Brazil. Figure 6: Percentage of inhibition for the growth of _X. campestris_ pv. _campestris_ due to the effect of cinnabarin from _P. sanguineus_ in different media at 7, 14 and 21 days. [PERSON] and [PERSON] [36] reported a growth rate of 9.9 mm/day, which was greater than that reported in the present investigation. It has been shown that the production of biomass is not related to the production of secondary metabolites with antimicrobial activity [34]. The secondary metabolites produced by the genus _Pycnotorus_ depend on the species and culture conditions assayed [10; 34]. After the evaluation period (21 days), it was observed that dissolved biomass, efficiency of the extract and zone of inhibition were important to evaluate the concentration of cinnabarin in the different media. The production of natural pigments by fungi has recently gained ground in the field of biotechnology. The variety of secondary metabolites produced by fungi could be associated with their incapacity to synthesize macromolecules from carbon dioxide or light energy, since they do not contain chlorophyll. Therefore, their survival depends on environmental conditions and the substrate where they develop [37]. [PERSON] [38] obtained 0.8501 g of dissolved biomass, which was higher than reported herein. The results did not show a clear tendency, since the best conditions for the CP-MaPs strain of _P. sanguineus_ were media enriched with dehydrated papaya, dehydrated sugarcane and glucose. According to [PERSON] et al. [22], the strain of _P. sanguineus_ MIP 89007 had a higher concentration of pigments at 20 days of incubation. The addition of dehydrated papaya, dehydrated sugar cane and glucose (3:1 \(v/v\)) to the culture medium affected the synthesis of cinnabarin.