## 3 Results and Discussion
### Abundance Heatmap Cluster of Different Microbial Classes  
According to the abundance information of 35 genera of all the samples, the heatmap was drawn to check whether the samples with similar processing were clustered, while the similarity of and differences in the samples can also be observed. _Bacteroidia_, _Gammaproteobacteria_, _Bacilli_, and _Chlostridia_ predominated in the Cs1.S.Pro soil (Figure 3). _Thermoanera-obaculia_, _Verucomicrobiae_, and _Hologhagea_ were the most abundant in the Cs2.S.Pro soil. _Methanosarcinia_, _Anaerolineae_, and _Planctomycetes_ dominated in the soil with the Cs3 cultivar of amelina when grown alone. An intercropping system with vetch stimulated the growth and distribution of _Nitrospira_, _Nitrososphaeria_, and _Blastocatellia_, involved in the oxidation of ammonia to nitrate via nitrite (Figure 4).  
Figure 2: (**A**) Number of observed species (97% OTUs) from 16S amplicons for each of the five soil samples. (**B**) Rank Abundance curves of alpha diversity.  
In the co-cultivation of camelina with peas, out of 30 bacterial classes that have the greatest influence on plant biomass degradation in the soil microbiome, were Actinobacteria, Rubrobacteria, Acidobacteria, KD4-96, Entotheonellia, and Chloroflexia. In contrast to the intercropping system with vetch, in the camelina-pea soil, the abundance of species from the phyla _Nitrosphaeria_ and _Nitrospha_ was low. _Nitrospha_ can utilize simple organic substrates, and they can survive in soil conditions where organic carbon is available and the paucity of nitrogen limits the _Nitrosomonadaceae_. When nitrogen fertilizer is applied, they may be less able to take advantage of the increased ammonia concentration, and their levels may decrease in the soil. Those results correspond to the findings of [PERSON] et al., 2019 [35]. There, they observed less pathogenic _Bacilii_ and _Clostridia_ in the Cs3.Ps.S.Pro soil compared to a sole cultivation of camelina. Thus, the application of camelina-pea intercropping has a beneficial role in suppressing soil-borne plant pathogens. [PERSON] et al., 2022 reported that the yield of pepper was positively correlated with the contents and the relative abundance of _Bacillus_ and _Clostridium_ in soil and was negatively correlated with the NO\({}_{3}\)-concentration in soil [36].  
From our results, soil bacterial communities varied in intercropping soil types with peas and vetch (Figure 4). Soil environmental factors are another factor explaining these soil bacterial community variations. Soil pH was most likely the primary factor determining differences in ammonia-oxidizing bacteria composition between different plots. Intercropping with legume plants improves the utilization of nutrient resources and the growth performance of intercrops [37]. Legume-based intercropping systems are known to increase microbial diversity and improve soil functioning, where nutrients become more available for plant roots [38]. [PERSON] et al. (1997) reported that legume-intercropping systems have beneficial effects on soil nutrient availability [39].  
Figure 3: A histogram of the relative abundance of taxa at the phylum level.