# Plant-Derived Bioactive Compounds for Rhabdomyosarcoma Therapy In Vitro: A Systematic Review
## 3 Results
### Plant Extracts and Isolated Bioactive Compounds That Induce Cell Death by Restricting Motility  
Only 5% of all plant extracts or bioactive compounds analyzed in this systematic review used inhibition of cell motility as a single mechanism or in combination with others [21; 23; 34] (Table 6). \begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multicolumn{1}{c}{**Raw Material**} & \multicolumn{1}{c}{**Extraction Method**} & \multicolumn{1}{c}{**Isolated Compound(s)**} & \multicolumn{1}{c}{**Cell Line**} & \multicolumn{1}{c}{**IC\({}_{\infty}\) Value of the Functional**} & \multicolumn{1}{c}{**Methods of Action**} \\  & & & & & **or Isolated Compounds** & \\ \hline \multirow{3}{*}{Fruits of _Chromographies_ _axilliars_[99]} & \multirow{3}{*}{Methanol} & \multirow{3}{*}{Cheorosponds A-E} & A-673 & RIR30 & Compound 1 induced inhibition of \\  & & & & & \\  & & & & & \\  & & & & & \\  & & & & & \\  & & & & & \\  & & & & & \\  & & & & & \\ \hline \multirow{3}{*}{Ranches and leaves of _Choversponalis axilliars_[50]} & \multirow{3}{*}{Methanol} & \multirow{3}{*}{Limonoids 1-8} & HEP-G2 & HEP-G2: 8.02 \(\pm\)9/mL & \multirow{3}{*}{Limonoids induced the formation of _R_D_ RD: 100.8 \(\pm\)9/mL & \multirow{3}{*}{RCDA-C7.} \\  & & & & & \\  & & & & & \\ \hline \multirow{3}{*}{Root of Morus allu [36]} & \multirow{3}{*}{Methanol} & Sanggrom G1 & T-ALL & \multirow{3}{*}{SG1 in OVCAR-4: 210 \(\mu\)M} & SC1 inhibits the XAP protein, _R_D_ RD: 100.8 \(\pm\)9/mL & leading to the initiation of apoptosis. \\  & & & & & \\ \hline \multirow{3}{*}{Root of Morus allu [36]} & \multirow{3}{*}{Methanol} & Sanggrom G3 & T-ALL & \multirow{3}{*}{SG1 in OVCAR-4: 210 \(\mu\)M} & SC1 inhibits the XAP protein, _R_D_ RD: 100.8 \(\pm\)9/mL & leading to the initiation of apoptosis. \\  & & & & & \\ \hline \multirow{3}{*}{Root of Morus allu [36]} & \multirow{3}{*}{Methanol} & Sanggrom G1 & T-ALL & \multirow{3}{*}{SG1 in OVCAR-4: 210 \(\mu\)M} & SC1 inhibits the XAP protein, _R_D_ RD: 100.8 \(\pm\)9/mL & leading to the initiation of apoptosis. \\  & & & & & \\ \hline \multirow{3}{*}{A-673 (Riving sarcoma): H-EP, IMR-32, and NS2 (Neuroblastoma cells): HPG-2 (Human hepatocellular carcinoma cells): MCTT (moncarbohydrate transporter 1); RD (Embrovascular rhudomsyosarcoma cells): SH00 (Alveolar rhabdomyosarcoma cells): SC1 (Sanggrom G1): T-ALL (T-cell lymphoblast leukemia): OVCAR-4 (Shabdomyosarcoma cells).} \\  & & & & \\ \hline \hline \end{tabular}
\end{table} 
Table 5: Plant extracts and isolated bioactive compounds induce cell death by blocking proteins. \begin{table}
\begin{tabular}{c c c c c c} \hline
**Raw Material** & **Extraction Method** & **Isolated Compound(s)** & **Cell Line** &
\begin{tabular}{c} **IC\({}_{\text{gg}}\) Value of the Functional** \\ **Extract** \\ **or Isolated Compounds** \\ \end{tabular} & **Mechanism of Action** \\ \hline Roots of _Vincetacular_ & & & RH-30 & RH-30 & 188.75\(\,\)ug/mL & Apoptosis induction by \\ _amentitatum_[21] & Methanol & & H-OH 1 & HA-OH1 & HA-OH1: 220.54\(\,\)ug/mL & PAYS-ROVO1 \\  & & Lupped & nMSC & nMSC: 1433.14\(\,\)ug/mL & Utility restriction by stabilization of \\  & & & & & \\  & & & & & \\  & & & & & \\  & & & & & \\  & & & & & \\  & & & & & \\  & & & & & \\  & & & & & & \\  & & & & & \\  & Ethanol 96\% & Heeane: palmitic & & & \\  & Heeane & acid extract & RD & & \\  & Chloroform & Chloroform: & & & & \\  & Ethyl acetate & scopoletin extract & & & \\  & & & & & \\ \hline  & MRSC: (Human healthy mesenchymal cells); PAYS-ROROOT (fusion gene) RD (Entropyonal rhabdomy sarcoma cells); RH-30 and H-COIT (Ause-lar rhabdomyosarcoma cells); SPII (Transcription factor). & & & \\ \end{tabular}
\end{table} 
Table 6: Plant extracts and isolated bioactive compounds that induce cell death by restricting motility. The bioactive compounds from the root of _Vinetoxicum arnottianum_, phytosterols (beta-sitosterol) and triterpenes (lupeol), were obtained using methanol. Its effect was studied in vitro in two alveolar RMS cell lines and one of the healthy human cells (RH-30, HA-OH 1, and hMSC, respectively).