## 3 Results
### Seasonal Changes of Microbial Biodiversity
#### 3.3.2 Seasonal Biodiversity of Bacterial and Fungal Communities
and _Rosomonas_ spp., as well as those from the families Hymenopteraaceae and Pseudonocardiacae, was found (Supplementary Table S2). The composition of the bacterial and fungal communities in samples from subsequent winter and summer seasons was also visualized using Principal Coordinates Analysis (PCoA). Our data indicate that the highest differences between seasons were documented in samples from sites 3, 4, 5, 7, and 8 for bacterial diversity, and from sites 4 and 5 for fungal diversity (Figure 8). Family proportion analysis underlines microbial biodiversity dependence from the BPs. Furthermore, this analysis establishes microbial taxa that are seasonally stable and where domination is strictly correlated to seasonal changes (Figure 8). It is worth noting that the seasonal variability of individual biocenoses resulted from differences in bacterial diversity. In the case of fungi, seasonal variability was observed mostly in samples from sites 4 and 5 (Figure 8). For stable biocenoses (sites 1, 2, and 6), the domination of the following bacterial families was observed: Sphingomonadaceae (sites 1 and 2), Chitinophagaceae, Micromonosporaceae, Tepidosphaeraceae, and Blastocatellaceae (site 1), Acetobacteraceae, Acidobacteriaceae (sites 2 and 6), and Sphingobacteriaceae (site 2) (Figure 7a). Interestingly, dominating taxa characteristic were observed only in summer or winter seasons despite the seasonal stability of bacterial families in the biocenoses listed above. In the sample from site 1, the following families were identified only during the summer period (Figure 7a): Beijernickiaceae, Isosphaeraceae, Pseudonocardiaceae, and Spirosomaceae; and during winter the Hymenopteraaceae family was identified in addition. For site 2 the Beijernickiaceae family was identified only during the summer period. For site 6 the Solibacteraceae family was identified only during the summer and Burkholderiaceae only during the winter season. Our data indicate that in the remaining five biocenoses (sites 3, 4, 5, 7 and 8) the microbial communities manifested significant seasonal changes (Figure 7). Bacterial families dominating only during the winter season included (Figure 7a): Burkholderiaceae, Clostridaceae 1, Cytophagaceae, Microsillaceae and Sphingomonadaceae (site 3) and Blastocatellaceae (site 4); Sphingobacteriaceae, Microcococcaceae, Nocardioidaceae and Micobacteriaceae (site 5); Marinobacteraceae, Cyclobacteriaceae, Halomonadaceae, and Cryomorphaceae (site 7); and Acetobacteraceae, and Burkholderiaceae (site 8). Specific to summer, dominating bacteria in biocenoses belonged to the families (Figure 7a): Trueperaceae and Caulobacteraceae (site 7); Rhodobacteraceae (sites 3, 4, and 5); Geodematophilaceae, Rhizoiaceae, and Isosphaeraceae (site 4); Acetobacteraceae (site 5); Beijernickiaceae (sites 3 and 5); Spirosomaceae (sites 3 and 8); and Flavobacteriaceae (site 8). The bacterial families occurring independently to the seasonal changes were (Figure 7a): Sphingomonadaceae (sites 1-5 and 7-8); Rhizoaceae (sites 5 and 7); Caulobacteraceae (site 4); Flavobacteriaceae (site 7); Hymenopteraaceae (sites 5 and 8); Sphingobacteriaceae (sites 7 and 8); Spirosomaceae and Tepidisphaeraceae (site 4); Microbacteriaceae, Nocardioidaceae and Geodematophilaceae (site 8); and Chitinophagaceae, Blastocatellaceae and Nostocaceae (site 3). Analysis of the distribution of fungal families demonstrated that their seasonal stability was associated with the dominance of (Figure 7b): Herptrichiellaceae (sites 2, 3 and 4); Bulleribasidiaceae (site 8); Parmeliaceae (site 6); Ramalinaceae (sites 4, 5 and 8); Teratosphaeriaceae (site 5); and Trichocomaceae (site 3). There were also fungal families which dominated in the summer season, i.e., Herptrichiellaceae (site 1); Physciaceae (site 5); and Ramalinaceae and Nectriaceae (site 7); or in the winter season: Teratosphaeriaceae (sites 1 and 6); and Stereocaulaceae (site 4).