## 3 Experiment and Results
### In Vitro Release of WPI-SNPs  
After determining the absorption transmittance morphological structure, magnetism, AC heating, and other characteristics, Adriamycin was attached to the modified superparamagnetic nanoparticles by electrostatic interaction between nanoparticles and reached an ideal condition of 15% drug loading capacity and 78.53% drug loading efficiency, which also fully explains that the WPI-modified superparamagnetic nanoparticles have a drug loading capacity of higher efficiency. It also supported our subsequent experiments. During conducting in vitro experiments using HepG2, cells were taken from mice, firstly biocompatibility analysis of WPI-\(\gamma\)-Fe\({}_{2}\)O\({}_{3}\), WPI-Fe\({}_{3}\)O\({}_{4}\)was performed, in vitro cytotoxicity test of HepG2 cell with WPI-\(\gamma\)Fe\({}_{2}\)O\({}_{3}\), WPI-Fe\({}_{3}\)O\({}_{4}\) was carried out at the concentration of 30, 60, 420, 240, 480 ug/mL using a Cell Counting Kit-8 analysis. HepG2 cell was treated with WPI-\(\gamma\)Fe\({}_{2}\)O\({}_{3}\), WPI-Fe\({}_{3}\)O\({}_{4}\) by 24 h. Figure 6a shows the cell survival rate proved that WPI-\(\gamma\)-Fe\({}_{2}\)O\({}_{3}\), WPI-Fe\({}_{3}\)O\({}_{4}\) showed high biocompatibility (non-cytotoxic).  
Attempts were made in culture, anatomy, and medicine, and the tumors were placed in the center of the AC electromagnetic coil and exposed to an AC magnetic field (\(f\) = 100 kHz, \(H\) = 1550e T (12.33 kA m\({}^{-1}\)), \(H\cdot f\) = 1.23 \(\times\) 10\({}^{9}\) A m\({}^{-1}\) s\({}^{-1}\)) for in vitro magnetic hyperthermia by taking out of the subcutaneous tissue of the mice and enveloping it with WPI-modified nanoparticles. The temperature of HepG2 injected with WPI-Fe\({}_{3}\)O\({}_{4(85 wt\odot)}\)  
Figure 5: (**a**) Induction heating devices and ThermalGun that measure temperature, (**b**,**c**) induction heating results of WPI-\(\gamma\)-Fe\({}_{2}\)O\({}_{3}\) and WPI-Fe\({}_{3}\)O\({}_{4}\) compared to the \(\gamma\)-Fe\({}_{2}\)O\({}_{3}\) and Fe\({}_{3}\)O\({}_{4}\); (**d**) heating results of WPI-\(\gamma\)-Fe\({}_{2}\)O\({}_{3(5 wt\odot)}\); (**e**) heating results of WPI-\(\gamma\)-Fe\({}_{2}\)O\({}_{3(6 wt\odot)}\); (**f**) heating results of WPI-Fe\({}_{3}\)O\({}_{4(5 wt\odot)}\); (**g**) heating results of WPI-Fe\({}_{3}\)O\({}_{4(85 wt\odot)}\); (**h**) hysteresis curve result of WPI-\(\gamma\)-Fe\({}_{2}\)O\({}_{3(6 wt\odot)}\) and WPI-Fe\({}_{3}\)O\({}_{4(85 wt\odot)}\); (**i**) the net magnetic moment of WPI-\(\gamma\)-Fe\({}_{2}\)O\({}_{3(6 wt\odot)}\) and WPI-Fe\({}_{3}\)O\({}_{4(85 wt\odot)}\).  
Figure 6: In vitro release of WPI-SNPs: (**a**) cells survival rate results of WPI-\(\gamma\)-Fe\({}_{2}\)O\({}_{3(67 wt\odot)}\) and WPI-Fe\({}_{3}\)O\({}_{4(85 wt\odot)}\) with HepG2 cell line; (**b**) HepG2 tumors were taken from mice for in vitro experiments; (**c**) HepG2 tumor after in vitro magnetic hyperthermia with WPI-\(\gamma\)-Fe\({}_{2}\)O\({}_{3(67 wt\odot)}\) and WPI-Fe\({}_{3}\)O\({}_{4(85 wt\odot)}\).  
and WPI-\(\gamma\)-Fe\({}_{2}\)O\({}_{3(67\mathrm{wt}\%)}\) was rapidly increased and stably saturated at \(\approx\)48.5 \({}^{\circ}\)C during the 15 s of in vitro hyperthermia. Finally, successfully treated HepG2 cells were taken from mice with in vitro hyperthermia. As shown in Figure 6b,c, it is seen that the HepG2 tumor after the hyperthermia not only become smaller but also has the surrounding surface burned and coagulated, losing the biological activity. The more exciting thing that a membrane of tiny lactose components formed around the surface of the tumor through WPI in the heating process, the lactose component is also one of the reasons to increase the heating rate.