## 4 Discussion  
It was established that different morphologies of AgNPs present different properties to one another. They can be synthesized in a range of shapes, including spheres, rods, wires, triangles, and cubes, each possessing unique characteristics. Spheres possess excellent stability and dispersibility, while in the case of TSNPs and other nanostructures with sharp geometries, it was observed that the tips can provide a greater electric field enhancement, allowing optical properties to be more easily tuned and controlled [17]. Furthermore, the same sharp edges and corners present in TSNPs make them more susceptible to oxidation, resulting in a higher rate of Ag+ ions released compared to spherical NPs, resulting in a higher antimicrobial activity [16]. In the solution form, color variations provide a visual means to differentiate and identify the specific shapes of silver nanoparticles: spheres are exhibiting yellow color while in the form of triangles the solution acquires a distinct blue (Figure 1). In the original protocol of [50] the concentration of silver is 16.692 ppm in the TSNP solution, where results show broad localized surface plasmon resonances (LSPR) of more than 130 nm full width at half maximum (FWHM) (Figure 2). The broad FWHM of the TSNP spectrum can be attributed to the inhomogeneity of sizes and nanoparticle shapes in the solution. This can reduce the nanoplate sensitivity, which is a critical property in TSNPs when used in nanosensing applications [56]. The triangular morphology of the TSNP prepared through this method was evaluated using TEM on a previous study performed by our team (Figure 3), where the same synthesis method for the TSNP was used [55]. The TEM images, the color, and the spectra readings of our TSNP solution come in accordance to other studies that used similar seed-mediated methods to synthesize TSNPs [5; 50; 54]. An increase in the produced volume and in the amount of silver in the nanoplate solution was achieved, and the resulting LSPR spectrum demonstrated that a reduced water amount does not have an impact on the LSPR behavior of the TSNP (Figures 4 and 5). It was  
Figure 13: (**a**) LSPR peak shift as a function of anti-Fn antibody concentration for Fn-PEGAuTSNPs (**b**) LSPR spectra of Fn-PEGAuTSNPs upon addition of increasing concentrations of anti-Fn. reported that high sensitivity of the TSNP LSPR is due to the high proportion of triangular geometries and the platelet structure of the nanoparticles. Furthermore, compared to single silver nanoparticles, the sensitivity is increased in highly organized silver nanoparticle aggregates (in solution) [56]. Silver nanoplates emerged as a remarkable tool in the development of antimicrobial materials, offering unique properties that make it a valuable asset in the fight against microbial infections. Through the incorporation of silver into diverse materials, such as coatings, textiles, and medical devices, significant advancements were made in the field of antibacterial therapeutics [57]. Taking into consideration previously mentioned properties and the lack of research on TSNPs for antimicrobial applications in polymeric materials, studies were performed to evaluate their suitability as antimicrobial agents for polymers and biopolymers. The TSNP synthesis method used in this study resulted in a highly concentrated TSNP solution, which is desirable in order to include the TSNPs into polymer processing. Using this method, the amount of water required to be removed was decreased, while the number of nanoplates available for the process was increased. Incorporation of TSNPs in biopolymer blends with a lower percentage of incorporated polymer (PHB) resulted in higher absorption of the aqueous TSNP solution by the hydrophilic BC matrix compared to the PLA and PCL blends that incorporated a higher percentage of polymer on the BC.