# Therapeutic Potential of Chrysin in Improving Bone Health
## 3 Results
### Effect of Therapeutic Interventions on 25-OH-D\({}_{3}\) by LC-MS/ms  
A significant (\(p<0.05\)) decrease in serum 25-OH-D\({}_{3}\) levels of animals treated with a vitamin-D-deficient diet was noticed compared with normal animals, which correlated with the altered biochemical and bone parameters. However, comparatively, there was a significant (\(p<0.05\)) increase in 25-OH-D\({}_{3}\) levels in serum samples of animals treated with other therapeutic interventions, i.e., III, IV, V, and VI, in contrast with group II animals (Figure 2).  
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multirow{2}{*}{**Parameters**} & \multicolumn{3}{c}{**Femur**} & \multicolumn{3}{c}{**Tibia-Fibula**} \\ \cline{2-7}  & **Length** & **Weight** & **Hardness** & **Length** & **Weight** & **Hardness** \\  & **(cm)** & **(gm)** & **(N)** & **(cm)** & **(gm)** & **(N)** \\ \hline Group I & 2.93 \(\pm\) 0.10 & 0.74 \(\pm\) 0.06 & 9.8 \(\pm\) 0.21 & 3.27 \(\pm\) 0.23 & 0.72 \(\pm\) 0.036 & 14.17 \(\pm\) 2.44 \\ Group II & 2.93 \(\pm\) 0.19 & 0.61 \(\pm\) 0.08 * & 8.94 \(\pm\) 0.12 * & 3.23 \(\pm\) 0.27 & 0.55 \(\pm\) 0.07 * & 6.98 \(\pm\) 1.91 * \\ Group III & 3.02 \(\pm\) 0.10 & 0.74 \(\pm\) 0.03 * & 9.93 \(\pm\) 0.24 * & 3.55 \(\pm\) 0.27 & 0.66 \(\pm\) 0.035 * & 11.91 \(\pm\) 2.10 * \\ Group IV & 3.08 \(\pm\) 0.12 & 0.74 \(\pm\) 0.06 * & 10.43 \(\pm\) 0.8 * & 3.60 \(\pm\) 0.21 & 0.74 \(\pm\) 0.066 * & 13.86 \(\pm\) 1.52 * \\ Group V & 3.03 \(\pm\) 0.15 & 0.79 \(\pm\) 0.05 * & 10.02 \(\pm\) 0.41 * & 3.57 \(\pm\) 0.38 & 0.75 \(\pm\) 0.096 * & 14.39 \(\pm\) 2.213 * \\ Group VI & 3.13 \(\pm\) 0.12 & 0.88 \(\pm\) 0.06 * & 11.76 \(\pm\) 0.67 * & 3.65 \(\pm\) 0.33 & 0.84 \(\pm\) 0.016 * & 15.60 \(\pm\) 2.70 * \\ \hline \hline \end{tabular} Note: Data were expressed as the mean \(\pm\) SD (\(n=6\)), analyzed by one-way ANOVA followed by [PERSON]’s multiple comparison test. * = \(p<0.05\) is considered statistically significant when comparing group, I vs. group II. * = \(p<0.05\) is considered statistically significant when compared with the other treatment groups (group III, IV, V, and VI) vs. diet control group.  
\end{table}
Table 4: Effect of therapeutic interventions on femur and tibia–fibula weights, lengths, and hardness.  
\begin{table}
\begin{tabular}{c c c} \hline \hline
**Groups** & **4 th Lumbar Hardness (N)** & **8 th Thoracic Hardness (N)** \\ \hline Group I & 14.64 \(\pm\) 0.96 & 15.66 \(\pm\) 1.64 \\ Group II & 10.66 \(\pm\) 0.71 * & 10.21 \(\pm\) 1.45 * \\ Group III & 12.96 \(\pm\) 0.85 * & 14.26 \(\pm\) 1.21 * \\ Group IV & 15.20 \(\pm\) 1.01 * & 14.94 \(\pm\) 1.99 * \\ Group V & 14.96 \(\pm\) 1.86 * & 15.21 \(\pm\) 0.98 * \\ Group VI & 15.62 \(\pm\) 1.59 * & 16.04 \(\pm\) 1.29 * \\ \hline \hline \end{tabular} Note: Data were expressed as the mean \(\pm\) SD (\(n=6\)), analyzed by one-way ANOVA followed by [PERSON]’s multiple comparison test. * = \(p<0.05\) is considered statistically significant when comparing group, I vs. group II. * = \(p<0.05\) is considered statistically significant when compared with the other treatment groups (group III, IV, V, and VI) vs. diet control group.  
\end{table}
Table 5: Effect of therapeutic interventions on 4 th lumbar vertebrae hardness and 8 th thoracic vertebrae hardness.