## 4 Discussion
Also, high root tissue density is negatively correlated with root life span, it means a faster degradation of roots with lower tissue density in soil [48]. Regarding the plant physiology, low root tissue density may indicate less metabolic expenditure with the respiratory rate of the roots and faster shoot growth rate [49]. On other hand, plant can reduce root tissue density by the formation of air spaces in the root cortex when it has low nitrogen or phosphorus availability [2; 50]. C and VT treatments had conditions that led to low general development comparing with the other treatments in the first experiment with arugia, one hypothesis is that these plants were in an environment with less plant growth promoting bacteria, reducing, for example, the N and P uptake. In the MC treatment, the low root tissue density was accompanied with a good plant development compared with the other treatments. In this case, root tissue density reflected not a worse plant development but a reduction of the necessity of high respiration rates by the roots. Although the treatments Ap, MC, and Bk did not show thinner roots than the control group on arugia and lettuce, in general, they had more root length, more root surface area, and less root specific length. These results may be attributed to the inoculated microorganisms and it leaded to a greater plant development and nutrient uptake when comparing to the control group. Our results show that the _A. platensis_ suspension in general improved the plants development and can also be used for agricultural systems improvements. There are corroborations in the literature with related benefits due to the inoculation of _A. platensis_ in arugia, eggplant, lettuce, maize, and onion [51; 52; 53; 54]. Micronutrient concentration increasing in plant parts has also been reported [55; 56]. In the present work, _A. platensis_ increased macro and micronutrients in arugia and lettuce, when compared with the control and VT groups, excepting for Zn in arugia, these results can be explained due to the roots development improving, which aids the plants to explore more efficiently the soil. The same rational thinking can be applied to the MC and Bk treatments. The first trial with the microbial consortium used in this study was carried out by [PERSON] et al. (2021) [13]. The authors isolated the microbial consortium from compost and applied in arugia, carrots, and radish. The MC significantly improved the fresh mass of the arugia, carrots, and radish in 2.5, 1.3, and 16 times, respectively. The increase in the arugia shoots dry weight in 2.98 and 1.94 times in the first and second experiment of the present study corroborates with it. On the other hand, while [PERSON] et al. (2021) [13] related an increase in carrots biomass, the present results do not show that. Although the authors made just one application of the MC, it can be worthy to do another application in the middle of the growing period, since carrots takes a longer time to be harvested than arugia and lettuce. This approach of applying the microorganisms more than one time could also be adopted for the Ap treatment, [PERSON] et al. (2016) [51] for example did four applications of _A. platensis_ in an experiment with eggplant. Bokashi, as an organic fertilizer, has a high content of organic matter and nutrients, in addition to the microorganisms [21]. The results with bokashi application show a general improvement on plan development when compared to the control group (Tables 1-3). Controversially, [PERSON] et al. (2010) [57] did not observe yield increase in a four-year experiment with potato, winter barley, alfalfa, and winter wheat, each one in a year as a rotation in temperate climate. The authors related some effects on soil traits but attributed it to the nutrient content in bokashi rather than the microorganisms, since the autoclaved bokashi treated group had similar results. The use of bokashi through badly executed management can harm a crop.