## 1 Introduction  
Low-temperature and low-pressure plasmas have shown significant applications in the biomedical field through bactericidal disinfection, primarily due to the reactive species generated by it [1, 2]. Plasma-produced energetic electrons, intense electric fields, and UV radiation, along with some specific reactive oxidant species, such as reactive oxygen (O), ozone (O\({}_{3}\)), and hydroxyl radicals (OH\({}^{\prime}\)), play a vital role in bactericidal degradation, depending upon their redox potential and the reaction kinetics of each reactive species [3, 4, 5]. These reactive species and the UV radiation, accompanied by intense electric fields and plasma heating, interact with the bacterial cells, causing cell membrane damage as well as the breakage of the chemical bonds of the double-strand DNA structure and the oxidation of protein [6, 7, 8, 9, 10, 11, 12]. Vibrio Cholera (_V. cholera_) is a Gram-negative, halophile rod-like species which is considered to be the root cause of several cholera pandemics worldwide as well as local outbreaks. Owing to the halophile nature of the species, it thrives in water and spreads out through the vegetation and the animals that feed on it. The World Health Organization (WHO) has confirmed the outbreak of cholera in different parts of world due to water-source contamination [13]. This contamination is mostly caused by fecal pollution by animal or human sources [14]. Any object coming in contact with these contaminated sources also becomes affected. The sterilization of such contaminated objects infected by infectious bacteria is an active field of research [15]. [PERSON] et al. studied the effects of various disinfectants against different household materials, such as glass, wood, carpets, etc., and concluded that the complete inactivation is a challenge. [PERSON] et al. devised a disinfectant against _V. cholera_ using aketeshie, a local Ghanian beverage [16]. Several other techniques, such as sono-photolysis, photo-catalysis, photo-electro-catalysis, etc., are amongst the noteworthy UV-based sterilization techniques [17]. [PERSON] et al. demonstrated the effectiveness of solar disinfection of water with the use of photo-Fenton oxidative reaction. H\({}_{2}\)O\({}_{2}\) generation within cells leads to the accumulation of hydroxyl radicals by photo-Fenton reaction, which eventually leads to cell damage [18; 19]. All of the presented methods have shown some disadvantages and limitations with respect to the volume of the object to be treated, the environmental effects, and the ease of access. LPP is amongst the few techniques that can produce UV, energetic electrons, and various radicals which are considered to be _V. cholera_ inhibitors [20]. Plasma sterilization has proven to be an effective mechanism for the degradation of V. cholera, as compared to the pure-chemical and traditional treatments. In this research, _V. cholera_ was treated by low-temperature and low-pressure plasma generated inside a stainless-steel sterilization chamber. It was observed that the inhibition of Gram-negative _V. cholera_ increases with the increase in the plasma treatment time. The addition of H\({}_{2}\)O\({}_{2}\) fumes in the plasma sterilization chamber resulted in the enhancement of the yield rate of ROS. To diagnose the effects of plasma treatment on _V. cholera_ degradation, different characterization techniques were adopted, including spectral diagnostics, counting the colony-forming units (CFUs), inactivation efficiencies, kill rate, and retardant and bactericidal effects [21; 22; 23]. The results proved that H\({}_{2}\)O\({}_{2}\)-assisted plasma discharge caused a reduction in colony-forming units (CFUs) and disabled the process of the regeneration of _V. cholera_, as well as DNA wall distortion. Figure 1 represents the graphical representation of the reaction of the plasma-generated reactive species with _V. cholera_ for its DNA distortion.