# Modifications of Wound Dressings with Bioactive Agents to Achieve Improved Pro-Healing Properties
## 3 Pro-Healing Wound Dressings
### The Effect of Natural Compounds on Skin Regeneration
#### 3.1.1 Curcumin-Loaded Biomaterials
In another study, different curcumin concentrations (0.2, 0.5, and 1.0% \(w/w\)) were loaded to produce fiber mats. The researchers demonstrated that the average fiber diameter increased along with the increasing curcumin content in the composition of the biomaterial. Cell proliferation and attachment were also improved as a result of increased curcumin concentration. Furthermore, the initial release of the compound was faster for biomaterials with higher curcumin content [57]. In turn, [PERSON] et al. [52] produced a chitosan/polyvinyl alcohol/curcumin membrane with desirable wound healing properties by varying the content of curcumin and chitosan. The greatest reduction in a wound size (52.33% on the 7 th day in a rabbit model) was obtained for the biomaterial containing the highest concentration of curcumin (30 mg). The produced biomaterial also possessed antibacterial (_P. multocida_, _S. aureus_, _E. coli_, and _B. subtilis_) and antioxidant activity. [PERSON] et al. [58] showed a production method of novel wound dressing using an electrospinning technique. The biomaterial was made of curcumin and polymeric mixture (PLA/PCL). The dressing material with 16% content of curcumin had strong antibacterial activity against _E. coli_ and _S. aureus_, however it decreased cell viability to 60% compared to the control sample. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Type of** & **Composition of the** & **Experimental Model** & **Biological Properties and** & **Limitations** & **Ref. ** \\
**B biomaterial** & **Composition of the** & **B** & **Experimental Model** & **Advantages** & **B** \\ \hline Film & Curcumin, chitosan, & In vitro (NHDF—normal human dermal fibroblast cell line, NCTC clone 929 cells—mouse subcutaneous cell line) & Enhanced mechanical properties, antioxidant activity & Slight reduction in water swelling [59] \\  & Curcumin, chitosan, & In vitro (NHDF—normal human dermal fibroblast cell line, NCTC clone 929 cells—mouse subcutaneous fibroblast cell line) & Enhanced collagen organization, supported wound contraction & Not provided [60] \\  & Curcumin, Ottawa, & In vitro (L929—mouse fibroblast cell line), In vivo (rat model) & Enhanced water absorption and drying time & Not provided [61] \\  & Curcumin, Ottawa, & In vitro (M5T3-E1—mouse cavarial preosteoblasts, MCF.7—human breast cancer cell line) & Anticancer properties & Reduced cell proliferation [62] \\  & Curcumin, Ottawa, & In vitro (MCST3-E1—mouse cavarial preosteoblasts, MCF.7—human breast cancer cell line) & Anticancer properties & Reduced cell proliferation [62] \\  & Curcumin, Ottawa, & In vitro (L929—mouse cavarial preosteoblasts, MCF.7—human breast cancer cell line) & Anticancer properties & Initial burst release of curcumin [53] \\  & Curcumin, Ottawa, & In vitro (L929—mouse cavarial preosteoblasts cell line) & Enhanced antibacterial activity (_E.coli_, _S. aureus_) & Initial burst release of curcumin [53] \\  & Curcumin & In vitro (L929—mouse cavarial preosteoblasts cell line) & Anticancer antibacterial activity (_E.coli_, _S. aureus_) & Initial burst release of curcumin [53] \\  & Curcumin & In vitro (L929—mouse cavarial preosteoblasts cell line) & Anticancer antibacterial activity (_E.coli_, _S. aureus_) & Initial burst release of curcumin [53] \\  & Curcumin & In vitro (L929—mouse cavarial preosteoblasts cell line) & Anticancer properties & Initial burst release of curcumin [53] \\  & Curcumin & In vitro (L929—mouse cavarial preosteoblasts cell line) & Anticancer antibacterial activity (_E.coli_, _S. aureus_) & Initial burst release of curcumin [53] \\  & Curcumin & In vitro (L929—mouse cavarial preosteoblasts cell line) & Anticancer antibacterial activity (_E.coli_, _S.