# Hake Fish Preservation Using Plant-Based Impregnated Polylactic Acid Food Films as Active Packaging
## 3 Results and Discussion
### Preservation of Hake Fillets
#### 3.2.2 Effects of Storage on the Physical and Chemical Properties
\begin{table}
\begin{tabular}{l l c c c c c} \hline \multicolumn{2}{l}{**Storage Time (Days)**} & **0** & **2** & **5** & **7** & **9** & **12** \\ \hline \multicolumn{2}{l}{Fresh} & & & & & & \\ \hline \(L^{*}\) & Control & 42.06 \(\pm\) 0.75 & 49.23 \(\pm\) 0.95\({}^{1}\) A & 43.24 \(\pm\) 0.33 AB & 40.85 \(\pm\) 2.39 B & 44.26 \(\pm\) 0.92 BC & 41.22 \(\pm\) 0.21 bB \\  & Impregnated film & & 47.88 \(\pm\) 0.93\({}^{1}\) A & 47.16 \(\pm\) 1.68 \({}^{1}\) bA & 41.26 \(\pm\) 0.20 B & 37.87 \(\pm\) 1.30 \({}^{1}\) aC & 34.70 \(\pm\) 0.97 \({}^{1}\) aD \\ \hline \(a^{*}\) & Control & \multirow{2}{*}{\(-3.005\pm 0.37\)} & \(-2.96\pm 0.23\) bA & \(-4.30\pm 0.34\)\({}^{1}\) B & \(-4.14\pm 0.08\)\({}^{1}\) B & \(-4.16\pm 0.21\)\({}^{1}\) bB & \(-3.31\pm 0.18\) bA \\  & Impregnated film & & \(-3.82\pm 0.20\)\({}^{1}\) aA & \(-4.99\pm 0.16\)\({}^{1}\) BC & \(-4.50\pm 0.31\)\({}^{1}\) AC & \(-5.36\pm 0.16\)\({}^{1}\) aBC & \(-4.38\pm 0.31\)\({}^{1}\) aAC \\ \hline \(b^{*}\) & Control & \multirow{2}{*}{\(-1.378\pm 0.64\)} & 1.42 \(\pm\) 1.04 AB & 0.34 \(\pm\) 3.01 AB & \(-0.08\pm 3.00\) aA & 3.53 \(\pm\) 0.28 \({}^{1}\) aB & 3.93 \(\pm\) 0.21 \({}^{1}\) b \\  & Impregnated film & & 6.69 \(\pm\) 0.39 \({}^{1}\) bA & 2.60 \(\pm\) 1.26\({}^{1}\) B & 6.04 \(\pm\) 1.64\({}^{1}\) bA & 6.05 \(\pm\) 0.21 \({}^{1}\) bA & 3.19 \(\pm\) 0.37 \({}^{1}\) A \\ \hline \multicolumn{2}{l}{Light skin} & & & & & \\ \hline \(L^{*}\) & Control & 7.18 \(\pm\) 0.02 & 73.06 \(\pm\) 0.06\({}^{1}\) aA & 66.53 \(\pm\) 0.19 \({}^{1}\) b & 57.61 \(\pm\) 1.07 \({}^{1}\) aC & 59.49 \(\pm\) 0.44 \({}^{1}\) CDa & 60.88 \(\pm\) 0.77 \({}^{1}\) bD \\  & Impregnated film & & 69.80 \(\pm\) 0.96 bA & 66.53 \(\pm\) 0.15\({}^{1}\) b & 59.74 \(\pm\) 0.85 \({}^{1}\) cD & 61.35 \(\pm\) 0.07 \({}^{1}\) cD & 57.68 \(\pm\) 0.89 \({}^{1}\) aD \\ \hline \(a^{*}\) & Control & \multirow{2}{*}{\(-1.58\pm 0.01\)} & \(-2.07\pm 0.01\)\({}^{1}\) A & \(-1.86\pm 0.08\) A & \(-1.96\pm 0.11\) aA & \(-0.79\pm 0.03\) bB & \(-3.86\pm 0.13\) C \\  & Impregnated film & & \(-1.81\pm 0.20\) A & \(-2.90\pm 0.01\)\({}^{1}\) b & -0.46\(\pm 0.24\)\({}^{1}\) bC & -2.33 \(\pm\) 0.01 \({}^{1}\) aD & -4.407 \(\pm\) 0.23 \({}^{1}\) c \\ \hline \(\mu^{*}\) & Control & \multirow{2}{*}{\(-0.22\pm 0.05\)} & 1.33 \(\pm\) 0.06 \({}^{1}\) aA & 0.08 \(\pm\) 0.58 aA & 1.82 \(\pm\) 0.94 \({}^{1}\) aB & 4.44 \(\pm\) 0.11 \({}^{1}\) bC & 6.74 \(\pm\) 1.30 \({}^{1}\) c \\  & Impregnated film & & 11.29 \(\pm\) 1.05\({}^{1}\) bA & 5.80 \(\pm\) 0.11 \({}^{1}\) bB & 10.61 \(\pm\) 0.16 \({}^{1}\) bA & 36.26 \(\pm\) 0.06 \({}^{1}\) aA & 4.74 \(\pm\) 2.88 \({}^{1}\) b \\ \hline \multicolumn{2}{l}{Dark skin} & & & & & \\ \hline \(L^{*}\) & Control & \multirow{2}{*}{\(46.88\pm 0.68\)} & 42.15 \(\pm\) 0.14 A & 33.07 \(\pm\) 0.40 \({}^{1}\) aB & 34.80 \(\pm\) 0.67 \({}^{1}\) bA & 32.56 \(\pm\) 3.88 \({}^{1}\) bB & 35.80 \(\pm\) 3.72 \({}^{1}\) b \\  & Impregnated film & & 47.79 \(\pm\) 1.30 A & 40.12 \(\pm\) 0.14 \({}^{1}\) bB & 35.63 \(\pm\) 0.84 \({}^{1}\) bC & 38.04 \(\pm\) 0.94 \({}^{1}\) b & 31.06 \(\pm\) 0.31 \({}^{1}\) c \\ \hline \(a^{*}\) & Control & \multirow{2}{*}{\(-1.53\pm 0.05\)} & 2.92 \(\pm\) 1.73 \({}^{1}\) b & 4.28 \(\pm\) 0.12 \({}^{1}\) b & 1.50 \(\pm\) 0.21 a & 2.62 \(\pm\) 0.70 \({}^{1}\) b & 2.10 \(\pm\) 0.91 \({}^{1}\) b \\  & Impregnated film & & \(-1.77\pm 3.39\) aA & 1.57 \(\pm\) 1.43 \({}^{1}\) aB & 3.26 \(\pm\) 0.01 \({}^{1}\) bB & 1.11 \(\pm\) 0.30 AB & 1.88 \(\pm\) 0.19 \({}^{1}\) b \\ \hline \(b^{*}\) & Control & \multirow{2}{*}{\(3.68\pm 0.19\)} & 10.95 \(\pm\) 1.51\({}^{1}\) A & 10.82 \(\pm\) 0.85 \({}^{1}\) A & 9.55 \(\pm\) 0.10 \({}^{1}\) aA & 11.66 \(\pm\) 1.00 \({}^{1}\) bA & 21.00 \(\pm\) 1.32 \({}^{1}\) bB \\  & Impregnated film & & 10.01 \(\pm\) 0.16 \({}^{1}\) aB & 12.15 \(\pm\) 0.05 \({}^{1}\) b & 15.12 \(\pm\) 1.41 \({}^{1}\) bC & 8.06 \(\pm\) 0.76 \({}^{1}\) aA & 12.83 \(\pm\) 1.47 \({}^{1}\) aBC \\ \hline \end{tabular}
\end{table} 
Table 3: CIELab parameters of the hake filters along storage time.