## 3 Results and Discussion
In order to characterize the polysaccharides and proteins in the hydrocolloid preparations extracted from milled fenugreek and basil seeds, SEC (size exclusion chromatography) analysis was carried out and molar mass distributions were obtained (Supplementary Figures S1 and S2), from which molecular parameters--weight average molar mass M\({}_{\text{w}}\), number average molar mass M\({}_{\text{n}}\), and dispersity B--were calculated, and the results are shown in Table 1.  
The molar mass of polysaccharides extracted from fenugreek seeds at both extraction temperatures (PFRT and PF70, Table 1) was similar to values reported in previous publications. Based on studies by [PERSON] et al. [7] and [PERSON] et al. [9], the molar mass of polysaccharides extracted from milled fenugreek seeds was 1,418,000-2,350,000 g/mol, and the difference in results presented in the literature could be explained by the use of purification of the extracts, as the protein-free samples had the highest molar mass [7; 9]. The molar mass of polysaccharides extracted from milled sweet basil seeds was lower (Table 1) than the molar mass of polysaccharides extracted in the study of [PERSON] al. [13] from unmilled basil seeds, which was estimated at 2,320,000 g/mol. The observed difference was mainly a consequence of using a different form of the raw material. As shown in Table 1 for both fenugreek and basil seed-extracted polysaccharides, the polysaccharides extracted with water at 70 \({}^{\circ}\)C had a lower molar mass compared to the polysaccharides extracted at room temperature. The likely reason for the lower molar mass of polysaccharides extracted at 70 \({}^{\circ}\)C compared to those extracted at room temperature, was the degradation of polysaccharide and polysaccharide-protein aggregates at the higher extraction temperature. Table 2 shows the results of antioxidant properties of non-starch polysaccharide preparations from milled fenugreek and sweet basil seeds obtained at room temperature and at 70 \({}^{\circ}\)C.  
All of the preparations studied were characterized by significant contents of polyphenols and flavonoids. This confirms the indications in the literature, according to which methanolic extracts from both fenugreek and basil seeds showed high contents of these bioactive components [14, 35, 36, 37, 38, 39]. Fenugreek seed extracts were characterized by a higher (\(p<0.05\)) content of polyphenols and flavonoids compared to basil seed extracts. Only the flavonoid content of the seed extracts of these 2 plants obtained at 70 \({}^{\circ}\)C was similar. Based on the results obtained (Table 2), it can be concluded that the preparations extracted at 70 \({}^{\circ}\)C from milled fenugreek and sweet basil seeds were characterized by a higher content of polyphenols and flavonoids. Antioxidants can be defined as reducing and/or anti-radical substances that counteract the damage to cells caused by reactive oxygen forms. The antioxidant activity of various substances of natural origin has been determined in countless experiments using many methods and under various conditions. Due to the wide variety of reactive oxygen forms and their mechanisms of action, none of the methods for determining antioxidant activity can be considered universal. Therefore, oxidative activity parameters are determined in studies in several ways [40, 41, 42]. Chemical (spectrophotometric) methods for determining antioxidant capacity based on the SET (Single-Electron Transfer) mechanism include synthetic radical scavenging capacity (ABTS and DPPH) and ferric ion reduction (FRAP). The ABTS radical is soluble in water and organic solvents, which allows determining the antioxidant capacity of both hydrophilic and lipophilic compounds/samples [43, 44]. DPPH is a radical that is soluble in organic media, so it is used for hydrophobic systems.