## 4 Discussion
Small Nuclear Ribonucleotideoprotein Polypeptide F (SNRPF) plays role in pre-mRNA splicing and also as a component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome [49; 50; 51; 52; 53; 54; 55; 56; 57]. The _SNRPF_ gene was found to be downregulated in our study samples and therefore may serve as a valid target for disease-based research. CTNNB1 or Catenin Beta 1 is an important downstream component of the canonical Wnt signaling pathway [58; 59; 60; 61; 62; 63; 64; 65]. The Wnt signaling pathway is known for its role in embryonic development, where it actively participates in body axis patterning, cell fate specification, cell proliferation, and cell migration events [66]. These developmental processes are essential for proper tissue formations, including bone, heart, and muscles. CTNNB1 protein is also a part of a protein complex that forms cell-cell junctions in epithelial and endothelial tissues [67]. Additionally, \(\beta\)-catenin 1 also promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle [68]. Surprisingly, \(\beta\)-catenin has also been associated with endometrial cancer onset and recurrence [69]. Therefore, it is evident from the above-mentioned studies that CTNNB1 has an important role in early developmental pathways and inter- and intracellular recognitions. Interestingly, this gene was found to be upregulated in our study, rendering it an important marker for disease-based research and for exploring its role in disease prognosis. Located on chromosome 5q35.3, HNRNPAB or Heterogeneous Nuclear Ribonucleoprotein A/Bis is a member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. HNRNPAB has also been found to be associated with ankyloblepharomectodermal defects-cleft lip/palate syndrome [70; 71; 72]. Surprisingly, this gene is also a member of the preimplantation embryo pathway (WP3527) [73]. In our study, this gene is downregulated, similar to the _SNRPF_ gene. Considering the above-mentioned facts, it can be hypothesized that HNRNPAB has a definitive role in disease prognosis via pre-mRNA processing or preimplantation embryo pathways and can be an essential diagnostic marker for endometriosis-based RPL. It is clearly evident from Table 4 that the top 20 pathways of the pathway enrichment analysis based on the overlapping genes of the EA-NA analyses are mainly concerned with signaling pathways and developmental biology, thereby indicating the combined inclination of the genes towards functioning in the arena of developmental signaling events during embryogenesis. When we tried to explore the involvement of our potential biomarkers in the biological pathways, it was seen that the SNRPF protein is involved in the cellular component organization pathway. Interestingly, CTNNB1 is involved in all 20 pathways. HNRNPAB is involved in 15 pathways and TWIST 2 in 13 of the pathways. CTNNB1, HNRNPAB and TWIST2 are commonly involved in 11 out of 20 major pathways, that are shown in Supplementary Table S1, while SNRPF and CTNNB1 share only one pathway in common.