## 2 Materials and Methods
### Bioactive Compounds  
For this, uniformly sized fruits were collected at stage 6 (light red) of maturity according to the color visual scale of USDA [22]. The fruits were selected, two per plant, verifying that they were not physically damaged and that they were uniform. These fruits were washed. Six fruits were used whole to perform the determinations in fresh tissue immediately, and six fruits were frozen at \(-80\)\({}^{\circ}\)C and then lyophilized for 72 h at \(-84\)\({}^{\circ}\)C and 0.060 mbar in a freeze dryer (Labconco, FreeZone 2.5 L model, Kansas City, MO, USA). These samples were ground to a fine powder to perform the determinations in lyophilized tissue. The vitamin C content was determined by the titration method with 2,6-dichlorophenol, using 1 g of fresh tissue and HCl (2%) [24]. The lycopene content was quantified according to the methodology of [PERSON] et al. [25] using 1 g of fresh tissue and hexane:acetone (3:2). The total phenols were determined according to the methodology of [PERSON] et al. [26]. The sample (0.2 g) was extracted with 1 mL of a water:acetone solution (1:1). The mixture was vortexed for 30 s. The tubes were centrifuged (Thermo Scientific Mod. ST 16R centrifuge, Langenselbold, Germany) at 17,500 \(\times\) g for 10 min at 4 \({}^{\circ}\)C. In a test tube, 50 \(\upmu\)L of the supernatant, 200 \(\upmu\)L of the Folin-Ciocalteu reagent, 500 \(\upmu\)L of 20% sodium carbonate (Na\({}_{2}\)CO\({}_{3}\)), and 5 mL of distilled water were added and then vortexed for 30 s. The samples were placed in a water bath at 45 \({}^{\circ}\)C for 30 min. Finally, the reading was taken at an absorbance of 750 nm using a plastic cell in a UV-Vis spectrophotometer (Thermo Fisher Scientific, G10S model, Waltham, MA, USA). The results were expressed in equivalent milligrams of gallic acid per 100 g dry weight (mg EGA 100 g\({}^{-1}\) DW). The flavonoids quantification was carried out using [PERSON]'s method adapted by [PERSON] et al. [27]. For the extraction, 100 mg of lyophilized tissue was placed in a test tube, where 10 mL of reagent grade methanol was added and shaken for 30 s until the mixture was homogenized. The mixture was filtered using No. 1 Whatman paper. For the quantification, 2 mL of the extract and 2 mL of methanolic solution of aluminum trichloride (AlCl\({}_{3}\)) 2% were added in a test tube and left to rest for 20 min in the dark. The reading was then taken in a UV-Vis spectrophotometer (Thermo Fisher Scientific, G10S model, MA, USA) at a wavelength of 415 nm using a quartz cell. The flavonoid content was expressed in equivalent milligrams of quercetin per 100 g of dry weight (mg EQ 100 g\({}^{-1}\) DW). The same extract was used to determine the total proteins [28], glutathione (GSH) [29], ascorbate peroxidase (APX) [30], glutathione peroxidase (GPX) [31], superoxide dismutase (SOD) (SOD Cayman 706002(r) kit [16]), catalase (CAT) [32], and the antioxidant capacity by 2,2\({}^{\prime}\)-azino-bis(3-ethylbenzthiazolin-6-sulfonic acid) (ABTS) [33]. For this, 200 mg of lyophilized tissue was placed in a 2 mL Eppendorf tube. Then, 20 mg of polyvinylpyrrolidone and 1.5 mL of phosphate buffer were added. The quantification of total proteins was determined using [PERSON]'s colorimetric technique, [28]. In a microplate, 5 \(\upmu\)L of the extract and 250 \(\upmu\)L of Bradford reagent were placed in each well. They were incubated for 10 min at room temperature (26 \({}^{\circ}\)C) and then read at a wavelength of 630 nm on a microplate reader (BioTek, ELx808 model, Winooski, VT, USA). The total proteins were expressed in mg g\({}^{-1}\) of DW. Glutathione quantification was performed using the spectrophotometric technique by [PERSON] et al. [29], by means of a 5,5-dithio-bis-2 nitrobenzoic acid (DTNB) reaction. A mix of 0.480 mL of the extract, 2.2 mL of sodium dibasic phosphate (Na\({}_{2}\)HPO\({}_{4}\) at 0.32 M), and 0.32 mL of the DTNB dye (1 mM) were placed in a test tube.