## Materials and Methods
### Sample Extraction  
The lyophilized samples of kiwifruit and persimmon cultivars were extracted with deionized water (pH 3) during 60 min at 25 \({}^{\circ}\)C. For mass spectrometry (MS) measurements the samples were extracted with ethanol: water mixture (8/2, _v/v_) during 60 min at 25 \({}^{\circ}\)C. The proportion of sample to the solvent was 1/10_w_/_v_. The extracts were filtered in a Buchner funnel. After removal of the solvent in a rotary evaporator and the aqueous solution were freeze-dried [25; 26].  
Figure 1: Investigated samples of kiwifruit: (**A–C**) _Actinidia_ (_A._) _delicious_ cv.Hayward (ADH), _A. arguta_ cv. [PERSON] (AAC), _A. [PERSON]_ cv. [PERSON] (AEB), and (**D**), _Diospyros kaki_ Thunb. cv. Fuyu (DKF).  
Total Contents (T) of Phenolics (TPs), Flavonoids (TFAs), Flavanols (TFLs), Tannins (TNs), Vitamin C (VC) and Main Secondary Metabolites  
The TPs content was determined by Folin-Ciocalteu colorimetric method [27], where 0.25 mL of phenolic extract solution (edible fraction) or standard was mixed with 1 mL of Folin-Ciocalteu reagent. In the next step 0.75 mL of 20% sodium carbonate was added and incubated for 6 min in a water bath at 45 \({}^{\circ}\)C. The absorbance of the resulting mixture was measured at 750 nm. Quantification of TPs in the samples was performed using a standard curve prepared with gallic acid, and the values were expressed as mg of gallic acid equivalent (GAE) per g dry weight (DW).  
The TFA content [28] was measured in the mixture of 0.5 mL of the extract with 2.25 mL of distilled water, followed by the addition of 0.15 mL of 5% (_w/v_) NaNO\({}_{2}\) solution. After 6 min, 0.3 mL of a 10% AlCl\({}_{3}\)-6H\({}_{2}\)O solution was added. The reaction was allowed to stand for another 5 min before addition of 1.0 mL of 1 M NaOH. The mixture was mixed well by vortexing, and the absorbance was measured immediately at 510 nm.  
TFLs were estimated using the _p_-dimethylaminocinnamaldehyde (DMACA) method, where 0.2 mL of fruit extract was introduced into a 1.5-mL Eppendorf tube, and 1 mL of DMACA solution was added. The mixture was vortexed and allowed to react at room temperature for 10 min. The absorbance at 640 nm was then read against a blank prepared similarly without DMACA. The presence of flavanols on the nuclei subsequent staining with the DMACA reagent resulted in an intense blue coloration in fruit extract [29].  
TNs were estimated by spectrophotometric measurements of 0.5 mL fruit extract, where 3 mL of a 4% methanol vanillin solution and 1.5 mL of concentrated hydrochloric acid were added [30]. The mixture was allowed to stand for 15 min. The absorption of samples and blank against water was measured at 500 nm.  
A catechin standard was used for the elaboration of the analytical curve and the results of TFAs and TNs contents were expressed as mg catechin equivalent (CE) per g DW. TFLs content was calculated as ug CE per g DW.  
VC (mg Asc per g DW) was evaluated in fruit extracts, where 100 mg of freeze-dried fruit sample was extracted with 5 mL water. Then cupric reducing antioxidant capacity (CUPRAC) method was conducted and formed bis (Nc)-copper (I) chelate was determined spectrophotometrically at 450 nm [31].  
The ethanolic extracts were submitted to MS analysis for main secondary metabolites determination and were processed exactly as described previously [1; 32]. A mass spectrometer, a TSQ Quantum Access Max (Thermo Fisher Scientific, Basel, Switzerland) was used. All samples were analyzed by direct infusion in the mass spectrometer by electrospray ionization (ESI) in negative mode, full scan analysis, range of 100-900 _m/z_. For optimization of the acquisition parameters and for identity confirmation, only a part of standards was employed, not for all compounds that were found in the investigated samples.