## 2 Results
### Composition and Variation of Floral Scent among Jaborosa Species  
Floral scent in the genus _Jaborosa_ is chemically complex, representing diverse biosynthetic pathways and reflecting substantial differences in emission strength. Accordingly, we used a combination of methods to collect and identify the VOCs present in the floral scent of the eight sampled _Jaborosa_ species. Dynamic headspace-GC-MS analyses of solvent-eluted samples led to the identification of 26 VOCs emitted by the night-bleoming flowers of _Jaborosa runcinata_ Lam. and _J. integrifolia_ (Table S2) during the first hour of anthesis, when hawkmoth visitation generally is most frequent. In contrast, this method was insufficient to characterize VOCs from day-bleoming flowers of _Jaborosa ratacea_ ([PERSON]) [PERSON] and [PERSON] and one of two replicates of _Jaborosa leucotricha_ ([PERSON].) [PERSON], revealing only trace amounts of 2-heptanone and p-methylanisole in the other replicate of _J. leucotricha_. Similarly, expanding dynamic headspace collection to 3 h led to the detection of trace amounts of only 11 VOCs from full inflorescences (15 flowers) of _Jaborosa sativa_ ([PERSON]) [PERSON] and [PERSON], a poor representation of the actual floral chemistry of this species (see Tables S3 and S4). The scent blend of _J. runcinata_ was simple, dominated by benzaldehyde (mean \(\pm\) s.e. of 92 \(\pm\) 5% of total emissions) and structurally related aromatic compounds (benzyl alcohol, benzyl benzoate, benzyl salicylate). These same compounds were present in the bouquet of _J. integrifolia_, albeit in different relative proportions, along with a more complex blend of aromatic esters (methyl benzoate, methyl salicate), oxygenated terpen (linalool, farnesol-related VOCs) and long-chain aliphatic aldehydes (Table S2). Chemical composition was consistent among samples collected from wild vs. greenhouse cultivated plants of _J. integrifolia_ across three years, whereas their relative emission rates per flower were  
Figure 1: Environmental suitability and occurrence map of the nightshade genus _Jaborosa_ Juss. The darkest color shades represent the areas with the highest suitability values for the Andean (blue) and Subtropical Lowland (red) clades. Phyto geographical regions (green lines) correspond to those provided by [73]. National boundaries delimited with black lines were obtained from [74]. roughly four-fold higher in cultivation than in natural settings (Table S2), potentially due to evening temperatures or other environmental factors. Direct thermal desorption-GC-MS solid-phase extraction (SPE) analyses were more effective in the collection and analysis of floral volatiles from _Jabrosa_ species of the Andean clade that grow in remote natural settings. For example, this method doubled the number of VOCs (25) detectable from flowers of _J. sativa_. A total of 84 VOCs were identified from trapped floral headspace of the eight studied species (Table S3). Floral VOCs from these species included 25 isoprenoid compounds (16 monoterpenes, 8 sesquiterpenenes, 3 irregular terpenoids), 21 aromatic compounds, 18 aliphatic compounds (esters, ketones, alcohols, aldehydes and alkenes), 11 ringed nitrogenous compounds (pyrroles, piperidines and indole) and 7 sulfutrous compounds (thioesters, oligosulfides and a thiophene; Table S3). Floral scent samples collected from two species of the Subtropical Lowland clade, _J.integrinia_ and _J. runcinata_, recovered nearly all of the compounds identified from solvent samples (Table S2), demonstrating internal consistency among methods for large, hawkmothpollinated flowers. Floral scent of the third species of the Subtropical Lowland clade, _Jabrosa donelliana_ [PERSON], was a simple blend dominated by acyclic sesquiterpene alcohols. Our data also indicate high interspecific variation in floral scent composition among the species of the Andean clade. Floral scent was a binary blend of oligosulfides in _J.