## 3 Results
### Analysis of Relative Shear Rates above an Adherent PMN and an Adherent TC  
For the purpose of comparing the impact of adherent cells on the local hydrodynamic environment between low and high shear conditions in the presence of sFg and sFn, relative shear rates were calculated as described [22], which is defined as local shear rate above an adherent cell divided by upstream wall shear rate. It was indicated that in the presence of sFg, most of the relative shear rates (with the state of \"transient aggregate\" an exception) above an adherent PMN under high shear condition are lower than those under low shear condition (Figure 3A). Meanwhile, it was also found that the highest Relative Shear Rate above an adherent PMN appears when a TC detaches from an adherent PMN, while the lowest Relative Shear Rate above an adherent PMN appears when a TC forms firm adhesion with an adherent PMN under both low and high flow conditions. The curve of relative shear rates above an adherent TC under high shear condition overlaps with that under low shear flow condition, and the lowest Relative Shear Rate also appears at the state of \"firm adhesion\" (Figure 3B). An interesting phenomenon is that the Relative Shear Rate above an adherent TC unexpectedly increases when a TC approaches an adherent PMN and forms transient aggregates with it.  
What would happen when sFg was converted to sFn in pathological blood flow? The curves of relative shear rates above an adherent PMN and an adherent TC do not show  
Figure 3: Relative shear rates above adherent cells in the presence of soluble fibrin (ogen). (**A**) Above an adherent PMN; (**B**) Above an adherent TC. Solid squares (\(\blacksquare\)) represent a low shear condition (91.25 \(\upmu\)L min\({}^{-1}\)) with sFg, solid circles (\(\bullet\)) represent a high shear condition (292 \(\upmu\)L min\({}^{-1}\)) with sFg, open squares (\(\cdot\)) represent a low shear condition (91.25 \(\upmu\)L min\({}^{-1}\)) with sFn, and open circles (\(\circ\)) represent a high shear condition (292 \(\upmu\)L min\({}^{-1}\)) with sFn.  
the same trend (the curves are overlapping) under both low and high shear conditions. As seen in Figure 3, the numeric range of relative shear rates above an adherent cell in the presence of sFn becomes smaller when compared with the numeric range that exists in the presence of sFg. The relative shear rates above an adherent PMN in the presence of sFn vary from 1.4 to 1.8 (from 1.2 to 2.0 compared with those in the presence of sFg) and the relative shear rates above an adherent TC vary from 1.9 to 2.5 (from 1.7 to 2.5 compared with those in the presence of sFg). More interestingly, the curves of relative shear rates above an adherent PMN and those above an adherent TC display similar trends when the shear condition is the same. Under a low shear flow condition, the Relative Shear Rate above an adherent cell reaches \"peak value\" at the states of \"approach\" and \"transient aggregates\", and \"valley value\" at the states of \"collision\" and \"firm adhesion\". Under high shear condition, the relative shear rates above an adherent cell at the latter three states are higher than those at the former three states.