## 3 Results  
The study aimed to evaluate the effectiveness of the exercises' protocol to smooth wrinkles. The evaluation was run by analyzing the scans overlapped at different follow-ups and examining their pixels with a colored scale. Blu hues indicate a volumetric reduction, while yellow-red shadows reveal an increased volume. Green color indicates no change. Of the 21 participants selected, one was lost at the second follow-up and a second was lost at the final follow-up. Images show a scan superimposed on an anatomical diagram of the main facial muscles as said above (Figure 2). In Figure 1, on the left, the overlay of the T0-T1 scans can be observed, while on the right, there is the overlay between T0-T3. From a purely visual analysis of the results obtained, in the T0-T1 overlay, no volumetric changes were observed. In fact, the highlighted muscle areas appear green, which means that no volumetric changes could be detected. In contrast, the T0-T3 overlay reveals that volumetric changes have developed in the zygomaticus muscle and the orbicularis or muscle. Figure 2: Overlapped scans with anatomical reference of the major muscle: (A) M. Procerus; (B) M. Orbicularis Oculi; (C) M. Levator Labii Superioris Alaeque Nasi; (D) M. Levator Labii Superioris; (E) M. Zygomaticus Minor; (F) M. Zygomaticus Major; (G) M. Buccinator; (H) M. Risorius; (I) M. Depressor Anguli Oris; (J) M. Mentalis; (K) M. Depressor Labii Inferiois; (L) M. Orbicularis Oris. On the left, superimposition is between T0–T1, on the right, it is between T0–T3. However, the statistical data obtained reveal that the two studied muscles did not behave in the same way. Regarding the zygomatic muscle, statistical analysis (Figure 3) found significant difference between the number of blue and green pixels with (\(p\) = 2.7 \(\times\) 10\({}^{-4}\)). The difference between the number of green and yellow pixels (\(p\) = 3.9 \(\times\) 10\({}^{-3}\)) was also significant. No significant difference between the number of blue and yellow pixels (\(p\) = 0.38) was revealed. This indicates that there was no significant volumetric change in the zygomatic muscle. In addition, the time variable is not significant either (\(p\) = 0.642). That is, it seems that it is not true that, with the passage of time, the exercise protocol proves to be more effective (Figure 4). As far as the orbicularis muscle is concerned, however, a statistically significant difference was revealed. Both the difference between the number of blue and green pixels (\(p\) = 0.019), and between the number of blue and yellow pixels (\(p\) = 5.3 \(\times\) 10\({}^{-5}\)), are significant (Figure 5). The amount of green and yellow pixels, when compared, shows no statistically significant difference (\(p\) = 0.067). Comparisons between treatment times were not significant (\(p\) = 0.688) (Figure 6). Figure 4: Graph of the trends of time in the zygomatic. 1 represents the blue range, 2 the green one, and 3 the red–yellow one. The blue line represents the period T0–T1, while the red one stays for the T0–T2 range, and the green one for the T0–T3 range. Figure 3: Graph of the trends of the three colors variables in the zygomatic muscles. 1 represents the blue range, 2 the green one, and 3 the red–yellow one. Taken together, these results indicate that a volumetric change of the orbicularis muscle was detected, while the zygomatic muscle appears to remain unchanged. The variable time does not appear to be significant. It seems that an increase in the number of exercises does not necessarily lead to an improvement in performance. Participants' responses to questionnaires on the satisfaction of their personal image and the aesthetic treatment they received were also analyzed. The questionnaire itself is reported in the Appendices A and B. The questionnaire data were obtained calculating the percentage difference before and after the treatment.