## 5 Conclusions  
Significant differences were found in the activity of different sources of CKs in shoot multiplication rates, direct root formation, and shoot branching morphology. Sucrose and Gelrite(tm) concentrations in the basal medium also contributed to these parameters. From the perspective of this work, one of the best ways to obtain higher yields of the EO of lemongrass would be to induce in vitro shoot multiplication with the combination of 2 iP and BAP in the culture medium, or 2 iP first followed by rooted shoot exposed to BAP. 2 iP induces a less branched phenotype than tZ, BAP, and KIN, although with stronger tillers and roots. We also conclude that established plants contained a significant amount of EO components by SPME/GC-MS analysis. Our results indicate that a BC of interest might be manipulated by different concentrations and sources of CK and the type of basal medium. For instance, 2 iP shoot morphology was associated with the lowest and BAP and KIN bushier shoot morphology with the highest content of \(\alpha\)-citral, the main constituent of the EO. Another conclusion from our results was that we could not state that direct root formation and development are related to EO production. Finally, many EO content and composition examples are influenced by the different growth and developmental stages of young to mature plants. Nevertheless, our results showed that plant morphology might also be related to the EO content and composition. This relationship is a new and compelling result not in the literature yet. The following supporting information can be downloaded at: [[https://www.mdpi.com/article/10.3390/ijpb13030025/s1](https://www.mdpi.com/article/10.3390/ijpb13030025/s1)]([https://www.mdpi.com/article/10.3390/ijpb13030025/s1](https://www.mdpi.com/article/10.3390/ijpb13030025/s1)), Figure S1: Germination of Cymbopogon citratus seeds; Figure S2: Shoot in vitro propagation; Figure S3: Chemical structure of CKs used in this work; Figure S4: Control in vitro plant grown on MS (3% Sucrose, 0.3% Gelrite(tm)) without any plant growth regulator. Figure S5: Micropropropagated plants of C. citratus; Figure S6: Plant morphology of in vitro shoot cultures of C. cittratus; Figure S7: Direct roots formed after two weeks of propagation; Figure S8: Direct roots formed after four weeks of propagation; Figure S9: Root induction in MS basal medium free of hormones; Figure S10: SPMG/GC-MS control profiles; Figure S9: In vitro Roots Phenotype; Table S1: Experimental Design. CK treatments statistical analysis was carried out under a multifactorial (2 \(\times\) 4 \(\times\) 3) ANOVA design; Table S2: Two-way analysis of variance for shoot number multiplication data (ANOVA); Table S3: Two-way analysis of variance for multiplication index data (ANOVA). Conceptualization, A.C.-R., J.L.C.-P. and A.M.-A. ; methodology, A.C.-R., J.L.C.-P. and R.V.-N.; formal analysis, R.V.-N. and A.C.-R.; investigation R.V.-N., A.C.-R., J.L.C.-P. and J.M.-T.; writing--original draft preparation A.C.-R., writing--review and editing, A.M.-A. and A.C.-R.; review, B.P.-A. ; project administration A.M.-A. ; funding acquisition A.M.-A., A.C.-R. and B.P.-A. All authors have read and agreed to the published version of the manuscript. **Funding:** This work was financed through the framework agreement CEIA/CEC/IRA/20210408/006 for collaboration in a research project between Ingredientes Especializados S.A. de C.V. and CINVESTAV-Irapuato. **Institutional Review Board Statement:** Not applicable. **Informed Consent Statement:** Not applicable. **Data Availability Statement:** Data generated in this research are presented within the article. **Acknowledgments:** We would like to thank Ingredientes Especializados S.A. de C.V. especially to [PERSON] and [PERSON] for financing the research project. We want to thank [PERSON] for her technical assistance in preparing culture media.