## 3 Results  
Changes in the Properties of Water after Incubation in the Presence of HD Abs to IFN\(\gamma\) under CMF Conditions  
In the first set of experiments (listed below in Sections 3.1 and 3.2), we studied samples prepared and supplied by OOO \"NPF \"MATERIA MEDICA HOLDING\". In the first experiment, we jointly incubated water samples (acceptor) with HD Abs to IFN\(\gamma\) (donor) in a controlled magnetic field generation device under conditions of CMF. Then, we used cellular chemiluminescence to assess the ability of water samples to affect the production of ROS by a suspension of neutrophils stimulated by fMLF. The degree of enhancement of the respiratory burst of neutrophils was 185% after a 60-min joint incubation (Figure 3). With a shorter joint incubation time (20 min), a stimulating effect (137%) was also noted, the magnitude of which was lower than with a longer exposure. For comparison, an experiment was performed in which water (acceptor sample) was incubated at a similar CMF, but in the absence of samples of donor (HD Abs to IFN\(\gamma\)). Other experimental conditions were the same. With this experimental setup, significant differences between the samples before and after 60-min incubation were observed (\(p<0.05\)), although at the earlier point (20 min) there was some stimulating effect, reaching 155% (Figure 3). However, this effect did not differ significantly from that of the sample that was incubated for 20 min in the presence of an HD Abs to IFN\(\gamma\). Thus, an ability of the donor (HD Abs to IFN\(\gamma\)) to have a moderate nonspecific physical effect on samples of acceptor (water) was demonstrated. Figure 3: Maximum chemiluminescence intensity of a suspension of neutrophils after the addition of water jointly or separately incubated with samples of HD Abs to IFN\(\gamma\). The samples contained: suspension of neutrophils—2.5 \(\times\) 10\({}^{5}\) cells; water incubated for 0, 20, and 60 min in the presence of HD Abs to IFN\(\gamma\) (hatched bar) or in the absence of HD Abs to IFN\(\gamma\) (white bar), which constituted 80% of the total sample volume; fMLF (2 uM); luminol (0.35 mM). Measurements were performed at 37 \({}^{\circ}\)C and pH 7.4; \({}^{\ast}p<0.05\) (intragroup comparison). Changes in the Properties of an IFN\(\gamma\) Solution after Incubation in the Presence of HD Abs to IFN\(\gamma\) under CMF Conditions  
It is well known that pre-incubation of a suspension of neutrophils with an IFN\(\gamma\) solution leads to cell activation and, ultimately, to a change in the production of superoxide radical [18]. An IFN\(\gamma\) solution was used as a specific target for HD Abs to IFN\(\gamma\)[19,20]. At this stage of the study, we assessed the ability of an IFN\(\gamma\) sample, after joint incubation with HD Abs to IFN\(\gamma\), to affect the production of ROS by an fMLF-stimulated neutrophil suspension. The experiments showed a significant (356%) increase in chemiluminescence in the suspension of neutrophils after a 1-h incubation in the presence of HD Abs to IFN\(\gamma\) (Figure 4). With a shorter (20 min) joint incubation of samples, a significant stimulating effect was also noted (270%), but its magnitude was lower than with a 60-min exposure (Figure 4). On the same day, an experiment was performed in which the IFN\(\gamma\) sample was incubated in CMF separately from HD Abs to IFN\(\gamma\) (under conditions that exclude distant interaction between these samples). The rest of the experimental conditions were the same. The IFN\(\gamma\) sample in this case also showed a significant chemiluminescence-stimulating effect (compared to the control sample--\"0 min\" of exposure taken as 100%), but this effect was noticeably less pronounced compared to those experiments where these samples were placed adjacent to samples of HD Abs to IFN\(\gamma\).