## 2 Results
### RNA Sequencing (RNA-seq) Analysis of Annotated Gene Transcripts
\begin{table}
\begin{tabular}{|l|l|l|l|l|} \hline
**Ensembl Gene ID** & **Gene Name** & **Description** & **Fold Change** & **FDR** \\ \hline
**EnsG00000196475** & **GK2** & **Glycerol kinase 2** & **-17.49** & **-22.37** & **72 h** \\ \hline
**EnsG00000164588** & **HCN1** & **Hyperpolarization activated cyclic nucleotide-gated potassium** & **-15.64** & **-23.39** & **6 h** \\ \hline
**EnsG00000101438** & **SLC32A1** & **Solute carrier family 32 (GABA vesicular transporter), member 1** & **-9.34** & **-68.18** & **72 h** \\ \hline
**EnsG0000178695** & **KCTD12** & **Potassium channel tetramerisation domain containing 12** & **-8.10** & **-41.92** & **6 h** \\ \hline
**EnsG0000182687** & **GALR2** & **Galanin receptor 2** & **-6.23** & **-17.14** & **6 h** \\ \hline
**EnsG0000120738** & **EGR1** & Early growth response 1 & **-5.33** & **-259.33** & **6 h** \\ \hline
**EnsG0000170989** & **EDG1** & **Endothelial differentiation, sphingolipid G-protein-coupled** & **-4.20** & **-21.82** & **72 h** \\ \hline
**EnsG0000186074** & **NKIR** & **NK inhibitory receptor precursor** & **-4.10** & **-17.18** & **72 h** \\ \hline
**EnsG0000153266** & **ZNF312** & **Zine finger protein 312** & **-3.91** & **-116.99** & **6 h** \\ \hline
**EnsG0000143595** & **AQP10** & **Aquaporin 10** & **-3.32** & **-97.38** & **72 h** \\ \hline
**EnsG0000153266** & **ZNF312** & **Zine finger protein 312** & **-3.01** & **-71.64** & **72 h** \\ \hline
**EnsG0000167183** & **PRPR15L** & **Proline rich 15-like** & **-2.95** & **-39.58** & **72 h** \\ \hline
**EnsG0000131470** & **TBIP** & **TBP-** **interacting protein (TBIP)** & **-2.80** & **-17.88** & **6 h** \\ \hline \end{tabular}
\end{table} 
Table 1: _Cont._ \begin{table}
\begin{tabular}{|l|l|l|l|l|l|} \hline
**Ensembl Gene ID** & **Gene Name** & **Description** & **Fold Change** & **FDR** & **Time Point** \\ \hline
**ENSG0000013489** & **CFHLS** & **Complement factor H-related 5** & \(-\)**2.72** & **0.37** & **48 h** \\ \hline
**ENSG00000156475** & **PPP2R2B** & **Protein phosphatase 2, regulatory subunit B (PR 52)** & \(-\)**2.65** & \(-\)**26.85** & \(72\) h \\ \hline
**ENSG0000159261** & **CLDN14** & **Claudin 14 (CLDN14)** & \(-\)**2.58** & \(-\)**26.90** & \(72\) h \\ \hline
**ENSG0000186910** & **SERPINA11** & **Serpin peptidase inhibitor, clade A** & \(-\)**2.53** & \(-\)**25.05** & \(72\) h \\
**ENSG0000182782** & **GPR109A** & **G protein-coupled receptor 109A** & \(-\)**2.50** & \(-\)**29.16** & \(72\) h \\ \hline
**ENSG0000182782** & **GPR109B** & **G protein-coupled receptor 109B** & \(-\)**2.50** & \(-\)**29.16** & \(72\) h \\ \hline
**ENSG0000197711** & **HP** & **Haptoglobin** & \(-\)**2.42** & \(-\)**291.19** & \(72\) h \\ \hline
**ENSG00000197711** & **HP** & **Haptoglobin-related protein** & \(-\)**2.42** & \(-\)**291.19** & \(72\) h \\ \hline
**ENSG00000130427** & **EPO** & **Eryrhropoietin** & \(-\)**2.39** & \(-\)**44.50** & \(72\) h \\ \hline
**ENSG00000143224** & **PPOX** & **Protoporphyinogen oxidase** & \(-\)**2.37** & \(-\)**15.81** & \(6\) h \\ \hline
**ENSG00000162344** & **FGF19** & **Fibroblast growth factor 19** & \(-\)**2.31** & \(-\)**40.79** & \(6\) h \\ \hline \end{tabular}
\end{table} 
Table 2: _Cont._The significantly differentially expressed genes in our RNA sequencing dataset were compared with a previously published comprehensive microarray dataset using a \(p\) value cutoff of \(<\)0.05 and fold change of 2 [9]. More than 80% of the differentially expressed genes at 48 and 72 hours in the RNA sequencing dataset were not significantly changed in the microarray analysis of poly(A) selected RNA of acutely HCV infected Huh 7.5 cells (Figure 2) [9]. Although minor differences in cell culture conditions might explain some of these differences, these results provide evidence that RNA sequencing analysis can provide new information regarding the changes in gene expression occurring during HCV infection. Approximately 37% of the genes that were significantly differentially expressed at 48 hours were also significantly changed at 72 hours.