## Materials and Methods
### Analitical Methods
#### 2.3.4 Determination of Bioactive Compounds  
Extract Preparation
Samples for chemical analyses were subjected to a freeze-drying process (30 \({}^{\circ}\)C, 0.630 mPa, 48 h). Then, the material was crushed in an analytical mill (IKA A11 basic; IKA-Werke GmbH, Staufen, Germany). Extraction was carried out using a solution of 80% ethyl alcohol and 0.1 M hydrochloric acid in the ratio of 85:15, _v/v_) on an orbital shaker (Multi Reax, Heidolph Instruments, Schwabac, Germany) for 12 h at 18 \({}^{\circ}\)C. The solution underwent centrifugation, and the resulting supernatant was carefully transferred to 0.2 mL PRC tubes. This extract was employed to assess the total phenols content (TPC), total anthocyanins content (TAC), and total antioxidant capacity. Two separate extracts were prepared for each sample. Total Polyphenols Content (TPC)  
A total of 10 uL of extract was placed on a 96-well plate and diluted twice with distilled water. A total of 40 uL of Folin-Ciocalteu reagent was added to the solution, and after 3 min 250 uL of supersaturated calcium carbonate was added also. The reaction was carried out for 60 min at 25 \({}^{\circ}\)C. The total polyphenolic content was quantitatively assessed through the measurement of absorbance in the reaction mixture at a 750 nm wavelength on multiple spectrophotometer (Multiksan Sky, Thermo Electron Co., Waltham, MA, USA), with reference to a calibration curve established for chlorogenic acid within the range of 0 to 100 ug/mL [40]. The analysis was performed in duplicate. Total Anthocyanins Content (TAC)  
The total anthocyanin content was determined spectrophotometrically by the pH differential method. A total of 30 uL of the extract was mixed with two buffers (pH 1 and pH 4.5) in an amount of 135 uL. After 20 min of incubation at 25 \({}^{\circ}\)C, the absorbance was measured against the reagent blank using a plate reader (wavelength: 510 and 700 nm). The analysis was conducted three times. The measurement was reported as milligrams of cyanide-3-glucose per gram of dry mass (mg/g d.m.) [41]. The analysis was performed twice. The vitamin C content was assessed via ultra-performance liquid chromatography (UPLC) using a Waters H-Class UPLC system and a photodiode array (PAD) detector (Waters, Milford, MA, USA) [42]. Approximately 0.05 g of material was extracted with a 10 mL milled solution composed of 3% metaphosphoric acid, 8% acetic acid, and 1 mM EDTA) for 10 min, followed by centrifugation at 5 \({}^{\circ}\)C for 5 min. The obtained supernatant was subsequently filtered through a syringe filter (0.2 um, GHP Acrodisc, Pall Corporation, Port Washington, NY, USA). Following a 2-fold dilution with the eluent, it was prepared for analysis. The separation procedure was carried out using a Waters HSS T3 C18 column (100 mm \(\times\) 2.1 mm, 1.8 um) at a temperature of 35 \({}^{\circ}\)C, with a mobile phase flow rate of 0.25 mL/min (consisting of 0.1% formic acid in Milli-Q water). The quantification of ascorbic acid content was accomplished by examining the spectrum at 245 nm and using a calibration curve established with the L (-) ascorbic acid standard. Each sample was analyzed in duplicate. Antioxidant Activity with DPPH and ABTS Radicals (AA)  
The antioxidant activities of strawberries samples were measured by Trolox equivalent antioxidant capacity assay, based on the ability of the antioxidants in the sample to scavenge the colored radical compared to the ability of the antioxidant vitamin E analog (Trolox) to scavenge radical. A solution of the green monocation radical 2,2'-azinobis-(3-ethylbenzothazoline-6-sulfonic acid) (19.2 mg ABTS + 3.3 mg K\({}_{2}\)S\({}_{2}\)O\({}_{8}\) + 5 mL H\({}_{2}\)O) and the purple radical diphenyllpicrylhydrazyl (25 mg DPPH + 100 mL CH\({}_{3}\)OH) was prepared. Immediately before analysis, the solutions were diluted to obtain absorbance on the spectrophotometer in the range of 0.680-0.720 AU, at wavelengths of 734, 515 nm, suitable for ABTS and DPPH.