## 2 Materials and Methods
### Effects of Different Amounts of Chicken Litter Biochar and Phosphorus on Plant Nutrient Uptake
Moreover, the use of CLB as an organic amendment in tropical acid soils has been reported to improve plant productivity through efficient plant nutrient absorption and translocation for dry matter production [42]. Treatment seven significantly increased N uptake in leaves, stems, and roots than those of T1, T2, T3, T4, T5, T6, T8, T9, T10, T11, and T12 (Table 5). [PERSON] et al. also reported a similar effect of biochar on N uptake [43]. The differences observed in leaves, stems, and roots nutrients uptake could be related to the physiology of maize plants. Higher accumulation of plant biomass occurs in the stems and followed by leaves [44]. \begin{table}
\begin{tabular}{c c c c} \hline \hline  & **Leaves** & **Stems** & **Roots** & **Total K Uptake** \\ \hline \multicolumn{4}{c}{**mg pot\({}^{-1}\)**} \\ \hline T1 & 43.65g \(\pm\) 0.70 & 24.43g \(\pm\) 0.66 & 53.86g \(\pm\) 1.18 & 121.95f \(\pm\) 1.78 \\ T2 & 371.37 cd \(\pm\) 12.30 & 730.99 cd \(\pm\) 4.70 & 196.85 cde \(\pm\) 10.59 & 1299.22c \(\pm\) 5.01 \\ T3 & 569.47a \(\pm\) 8.81 & 824.61 bc \(\pm\) 4.86 & 212.48 cd \(\pm\) 5.65 & 1606.56b \(\pm\) 18.47 \\ T4 & 446.77b \(\pm\) 11.79 & 1323.34a \(\pm\) 36.95 & 229.98c \(\pm\) 7.82 & 2000.10a \(\pm\) 40.67 \\ T5 & 538.21a \(\pm\) 20.91 & 994.76b \(\pm\) 25.96 & 171.03e \(\pm\) 0.99 & 1703.99b \(\pm\) 34.34 \\ T6 & 421.73 bc \(\pm\) 3.23 & 693.26 cde \(\pm\) 7.05 & 184.90 de \(\pm\) 5.58 & 129.99c \(\pm\) 15.17 \\ T7 & 426.42 bc \(\pm\) 12.94 & 948.88b \(\pm\) 26.62 & 194.95 cede \(\pm\) 14.50 & 1570.26b \(\pm\) 31.10 \\ T8 & 306.03 ef \(\pm\) 11.56 & 586.16 def \(\pm\) 12.51 & 174.76e \(\pm\) 5.95 & 1066.949 de \(\pm\) 2.51 \\ T9 & 338.01 de \(\pm\) 12.56 & 476.28f \(\pm\) 14.15 & 106.32f \(\pm\) 2.65 & 920.62e \(\pm\) 8.78 \\ T10 & 361.55 de \(\pm\) 10.37 & 505.92 ef \(\pm\) 6.29 & 206.56 cde \(\pm\) 6.15 & 1074.05 de \(\pm\) 21.39 \\ T11 & 303.67 ef \(\pm\) 50.90 & 557.40 def \(\pm\) 10.06 & 406.97a \(\pm\) 3.14 & 1268.03 cd \(\pm\) 7.32 \\ T12 & 269.66f \(\pm\) 18.02 & 562.00 def \(\pm\) 12.26 & 343.22b \(\pm\) 4.31 & 1174.89 cd \(\pm\) 129.07 \\ \hline \hline \end{tabular} Note: Different letters within a column indicate significant difference between means of three replicates \(\pm\) standard error using Tukey’s test at \(p\leq\) 0.05.  
\end{table} 
Table 6: Effects of treatments on potassium uptake of leaves, stems, and roots, and total potassium uptake of maize at tasseling stage. \begin{table}
\begin{tabular}{c c c c c} \hline \hline  & **Leaves** & **Stems** & **Roots** & **Total N Uptake** \\ \hline \multicolumn{4}{c}{**\% pot\({}^{-1}\)**} \\ \hline T1 & 6.44h \(\pm\) 0.19 & 1.44 d \(\pm\) 0.17 & 4.75e \(\pm\) 0.08 & 12.62f \(\pm\) 0.11 \\ T2 & 36.75 dce \(\pm\) 0.4 & 17.05c \(\pm\) 0.38 & 16.45 ab \(\pm\) 0.87 & 70.2d \(\pm\) 1.1 \\ T3 & 17.53g \(\pm\) 0.47 & 14.6c \(\pm\) 0.59 & 12.05d \(\pm\) 1.2 & 44.18e \(\pm\) 1.21 \\ T4 & 34.62 def \(\pm\) 0.34 & 24.86b \(\pm\) 1.41 & 13.91 bcd \(\pm\) 0.53 & 73.39d \(\pm\) 1.77 \\ T5 & 43.43 ab \(\pm\) 0.45 & 24.78b \(\pm\) 2.82 & 14.16 bcd \(\pm\) 0.88 & 82.34 bc \(\pm\) 1.49 \\ T6 & 29.1f \(\pm\) 1.63 & 25.73g \(\pm\) 0.71 & 19.4a \(\pm\) 0.52 & 74.24 cd \(\pm\) 0.82 \\ T7 & 46.66a \(\pm\) 2.36 & 36.26a \(\pm\) 1.48 & 16.07 abc \(\pm\) 0.43 & 98.99a \(\pm\) 3.07 \\ T8 & 33.03 def \(\pm\) 1.75 & 24.57b \(\pm\) 0.78 & 11.87d \(\pm\) 0.96 & 69.47d \(\pm\) 1.43 \\ T9 & 38.98 bcd \(\pm\) 2.05 & 17.61c \(\pm\) 0.31 & 12.64 cd \(\pm\) 0.5 & 69.23d \(\pm\) 2.32 \\ T10 & 36.86 cde \(\pm\) 1.71 & 24.62b \(\pm\) 1.34 & 12.7 cd \(\pm\) 0.5 & 74.19 cd \(\pm\) 1.58 \\ T11 & 45.53 ab \(\pm\) 1.43 & 34.18a \(\pm\) 0.49 & 10.6d \(\pm\) 0.76 & 90.31d \(\pm\) 0.54 \\ T12 & 30.91 ef \(\pm\) 0.59 & 32.05a \(\pm\) 0.96 & 11.49d \(\pm\) 0.41 & 74.45 cd \(\pm\) 1.19 \\ \hline \hline \end{tabular} Note: Different letters within a column indicate significant difference between means of three replicates \(\pm\) standard error using Tukey’s test at \(p\leq\) 0.05.  
\end{table}