Models derived with biomimetic properties gave better statistics and superior predictive performance from those based on calculated physicochemical properties. Overall, biomimetic chromatography can be suggested as a promising method in order to screen or rank chemicals in regard to their ecotoxicological risk. ## References  
* Fawell and Ong (2012) Fawell, J.; Ong, C.N. Emerging Contaminants and the Implications for Drinking Water. _Int. J. Water Resour. Dev._**2012**, _28_, 247-263. * Daughton (2004) Daughton, C.G. Non-regulated water contaminants: Emerging research. _Environ. Impact Assess. Rev._**2004**, _24_, 711-732. Valko, K.L. Volume 8. Separation Methods in Drug Synthesis and Purification. _Handb. Anal. Sep._**2020**, \(8\), 667-758. * Stergiopoulos et al. (2019) Stergiopoulos, C.; Makarouni, D.; Tsanttili-Kakoulidou, A.; Ochsenkuhn-Petropoulou, M.; Tsopelas, F. Immobilized artificial membrane chromatography as a tool for the prediction of ecotoxicity of pesticides. _Chemosphere_**2019**, _224_, 128-139. * Malligorski (2017)**P. Kalligosfyi 1, S. Nikou 2, S. Karteri 3, H. Kalofonos 3, V. Bravou 2 and D. Kalogianni 1,*
* 1 Department of Chemistry, University of Patras, 26504 Patras, Greece
* 2 Department of Medicine, University of Patras, 26504 Patras, Greece
* 3 Division of Oncology, Department of Internal Medicine, University Hospital of Patras, 26504 Patras, Greece
* * Correspondence: kalogian@upatras.gr  
Liquid biopsy is a non-invasive approach alternative to that of conventional tissue biopsy for cancer diagnosis and real-time monitoring of the disease. Liquid biopsies target specific biomarkers, such as blood circulating tumour cells (CTC), circulating tumour DNA (ctDNA), circulating microRNAs, and exosomes that are secreted by the tumours. Body fluid samples, such as blood sample, are used in liquid biopsies [1]. The presence of circulating tumour DNA (ctDNA) or cell-free DNA (cfDNA) in blood circulation has been related to early cancer detection and disease monitoring for proper treatment. The detection and quantification of cfDNA is a great analytical challenge due to its low molecular weight, its extremely low abundance in blood circulation, and the need for the discrimination of ctDNA that carries specific tumour-related gene mutations from normal cfDNA. Therefore, the discrimination of ctDNA from normal cfDNA is based on the detection of specific gene mutations that are located only at the tumour DNA [2; 3; 4]. In this project, a new rapid multiplex lateral flow assay was developed for the simultaneous detection of three major single-point mutations of the KRAS gene, related to colorectal cancer, in ctDNA, as well as the normal cfDNA. The multiplex test was initially optimized with synthetic DNA targets and cell lines that express both the normal and the mutated gene. The test was then successfully applied to tissue samples and blood serum samples for cfDNA detection. The proposed lateral flow assay offers a simple, cost-effective, and rapid alternative of liquid biopsy that is completed within 15 min, while the detection is accomplished by bare eye. This lateral flow assay offers multiplex detection of single point mutations, while it is universal and can be applied in the future for the detection and identification of several different kinds of cell-free or circulating tumour DNA molecules. ## References  
* Palmirotta et al. (2018) Palmirotta, R.; Lovero, D.; Cafforio, P.; Felici, C.; Mannavola, F.; Pelle, E.; Quaresmini, D.; Tucci, M.; Silvestris, F. Liquid biopsy of cancer: A multimodal diagnostic tool in clinical oncology. _Ther. Adv. Med Oncol._**2018**, _10_, 1-24. * Jia et al. (2017) Jia, S.; Zhang, R.; Li, Z.; Li, J. Clinical and biological significance of circulating tumor cells, circulating tumor DNA, and exosomes as biomarkers in colorectal cancer. _Oncotarget_**2017**, \(8\), 55632-55645. * Das and Kelley (2019) Das, J.; Kelley, S.O. High-performance nucleic acid sensors for liquid biopsy applications. _Angew. Chem. Int. Ed._**2019**, _58_, 2-13. * Gorgannezhad et al.