## 3 Results and Discussion
### Cytotoxicity
\begin{table}
\begin{tabular}{c c c c c c c c c c} \hline \hline
**Extracts** & \multicolumn{6}{c}{**IC\({}_{00}\) (ng/mL)**} \\ \hline  & **MCF7** & **SI** & **HEPG2** & **SI** & **A549** & **SI** & **HELA** & **SI** & **VERO** \\ \hline
**Waller Siusu** & \multicolumn{6}{c}{} \\ Acotone & \(197.3\pm 26.5\) & 0.6 & \(\mathbf{14.5\pm 0.2}\) & \(\mathbf{8.6}\) & \(147.4\pm 9.6\) & 0.8 & \(87.2\pm 9.6\) & 1.4 & \(126.1\pm 4.5\) \\ H\({}_{3}\)O (cold) & \(487.8\pm 11.9\) & 0.9 & \(832.1\pm 42.1\) & 0.5 & \(355.1\pm 59.5\) & 1.3 & \(946.6\pm 104.9\) & 0.5 & \(449.5\pm 0.8\) \\ H\({}_{2}\)O (hot) & \(418.7\pm 175.4\) & 0.5 & \(455.8\pm 24.1\) & 0.5 & \(830\pm 60.9\) & 0.3 & \(911.6\pm 56.6\) & 0.2 & \(214.3\pm 15.1\) \\ UPhfield & \multicolumn{6}{c}{} \\ Acotone & \(194.7\pm 27.2\) & 0.6 & \(\mathbf{8.6\pm 0.8}\) & \(\mathbf{14.2}\) & \(64.1\pm 20.4\) & 1.9 & \(\mathbf{34.5\pm 6.9}\) & **3.5** & \(122.1\pm 6.1\) \\ H\({}_{3}\)O (cold) & \(>\)1000 & 0.3 & \(754.6\pm 22.2\) & 0.7 & \(>\)1000 & 0.2 & \(>\)1000 & 0.4 & \(535.3\pm 20.5\) \\ H\({}_{2}\)O (hot) & \(666.7\pm 109.6\) & 1.5 & \(372.2\pm 8.3\) & 2.74 & \(490.8\pm 117.1\) & 2.1 & \(>\)1000 & 1 & \(>\)1000 \\
**Small** & \multicolumn{6}{c}{} \\ Acotone & \(23.3\pm 6.6\) & 0.7 & \(85.8\pm 6.8\) & 0.2 & \(166.9\pm 20.6\) & 0.0 & \(99.6\pm 4.9\) & 0.2 & \(\mathbf{16.1\pm 0.7}\) \\ H\({}_{3}\)O (cold) & \(764.1\pm 18.9\) & 0.6 & \(>\)1000 & 0.5 & \(188.7\pm 12.3\) & 2.6 & \(8024.1\pm 169.8\) & 0.6 & \(4859.1\pm 12.9\) \\ H\({}_{3}\)O (hot) & \(>\)1000 & 0.4 & \(607\pm 146.9\) & 0.8 & \(>\)1000 & 0.2 & \(694.5\pm 61.1\) & 0.7 & \(4644.4\pm 90.3\) \\ CHG\({}_{3}\) fraction & \(357.6\pm 11.9\) & 0.0 & \(213.3\pm 18.8\) & 0.1 & \(129.4\pm 25.4\) & 0.2 & \(672.2\pm 5.6\) & 0.5 & \(32.6\pm 3.1\) \\ Hexene fraction & \(167.5\pm 34.6\) & 0.2 & \(295.4\pm 18.1\) & 0.1 & \(250.9\pm 34.9\) & 0.1 & \(971.6\pm 81.3\) & 0.0 & \(378.4\pm 4.2\) \\ \hline
**Neispuri** & \multicolumn{6}{c}{} \\ Acotone & \(269.8\pm 33.2\) & 0.4 & \(248.4\pm 38.9\) & 0.40 & \(374.7\pm 8.4\) & 0.27 & \(>\)1000 & 0.07 & \(1003.0\pm 0.8\) \\ H\({}_{3}\)O (cold) & \(>\)1000 & 0.7 & \(246.6\pm 4.6\) & 3.01 & \(961.5\pm 137.1\) & 1.0 & \(>\)1000 & 0.71 & \(961.5\pm 19.2\) \\ H\({}_{3}\)O (hot) & \(658.16\pm 12.1\) & 0.5 & \(550.9\pm 70.4\) & 0.99 & \(136.6\pm 17.8\) & 2.4 & \(>\)1000 & 0.18 & \(322.5\pm 85.9\) \\ CHG\({}_{3}\) fraction & \(284.2\pm 38.4\) & 1.0 & \(38.5\pm 3.4\) & **8.5** & \(218.9\pm 9.9\) & 1.3 & \(842.5\pm 199.1\) & 0.34 & \(2842.61\pm 68.1\) \\ Hexene fraction & \(189.2\pm 12.8\) & 1.1 & \(312.4\pm 16.7\) & 0.7 & \(188.4\pm 33.4\) & 1.1 & \(153.1\pm 2.1\) & 1.3 & \(203.2\pm 2.5\) \\ \hline
**Oblieumont** & \(454.2\pm 57\) & 0.7 & \(52.7\pm 4.8\) & 6 & \(192.7\pm 1.6\) & 1.6 & \(188.5\pm 1.6\) & 1.7 & \(3148.4\pm 2.1\) \\ Luged \(k\) \(\&\)\(6\)\(\&\)\(\)\(\)\(\)\(\)\(\)\(\)\(\)\(\)\(\)\(\)\(\)\(\)\(\)\(\The main objective of cancer therapy is to use compounds that can specifically target cancer cells without toxicity against normal cells. Thus, the selective toxicity of extracts, fractions or compounds against cancer cells must be considered during discovery of leads for cancer treatment [37]. We therefore determined if _P. obliquuum_ acetone extracts, fractions and isolated compounds had selective activity to cancer cells. Tested samples with SI > 2 were considered to have selective toxicity against the tested cancer cell line [38; 39]. Acetone extract results also had selective cytotoxic activity against HepG2 and HeLa cancer cell lines with selective index values as high as 14 (Table 1). The Hatfield acetone extract showed the highest cytotoxic activity with IC\({}_{50}\) of 8.4 \(\upmu\)g/mL against HepG2 cells, and had the highest SI value of 14, which means that the extracts were approximately 7 times more toxic to cancer cells than normal Vero cells.