## 4 Discussion
While success in football is influenced by a wide variety of factors, including genetics, there is no specific \"power and sprint gene\" [28]. However, there are certain genes and genetic profiles that may be associated with the physical characteristics relevant to explosive strength in football that more accurately define the influence of genetics on performance and injury risk [13, 28, 29], as well as explosive strength [30]. Some of the genes that have been linked to these abilities to date include _ACTN3_ and _ACE_ polymorphisms, which can influence muscle composition, muscle injuries, and the ability to generate explosive force [12, 31]. However, in our study, only the c.1729C>T polymorphism of the _ACTN3_ genehas shown an association with the response in concentric mean force, with the TT genotype showing a higher frequency in non-responders. The _AMPD1_ gene has been the subject of interest in relation to athletic ability and sports performance, particularly in sports that require explosiveness [32]. The c.34C\(>\)T polymorphism is associated with the increased activity of the AMPD enzyme. This higher activity could theoretically lead to a greater ability to produce ATP (adenosine triphosphate), which is the main source of energy used during explosive efforts, associating the CC genotype and C allele with a greater ability to produce ATP and sprint/power skills [32]. However, studies on the relationship between the _AMPD1_ gene and athletic performance are inconclusive. Some studies suggest that athletes with the CC genotype of the _AMPD1_ gene may have an advantage in sports that require rapid energy release [33], while others have found no significant associations [34]. In this study, the only association with neuromuscular performance presented by the _AMPD1_ gene was in the concentric mean force in which there was a high frequency of non-responders in the CC and CT genotypes. The fact that there were amateur footballers could be one of the reasons for the lack of association and the small sample size presented, which justifies carrying out future studies in professional football players to study the results presented in greater depth. The _MLCK_ gene encodes an enzyme that plays a key role in the regulation of muscle contraction, specifically myosin light chain phosphorylation, which activates myosin and triggers muscle contraction. Some studies have suggested that the _MLCK_ c.37885C\(>\)A polymorphism may be associated with differences in the response to muscle damage after exercise, increasing susceptibility to muscle damage, especially in the AA genotype. In our study, it has been shown that the \"worst\" genotype for muscle performance (AA) showed a higher frequency among non-responders in the concentric mean force. In this case, the c.37885C\(>\)A polymorphism of the _MLCK_ gene together with the c.1729C\(>\)T polymorphism of the _ACTN3_ gene have been associated with muscle damage [35]. However, the results are still mixed and not all studies have found a significant association between the _MLCK_ c.37885C\(>\)A polymorphism and muscle damage. As we found these results to be promising, it will be necessary to implement them in professional athletes in which the demands of training and competitions are higher and in which it has previously been shown that this muscle damage may be associated with muscle injuries and setting in professional footballers [29]. Although the association of the c.*800A\(>\)G polymorphism of the _CKM_ gene in professional football players' injuries and performance has been previously presented [33], in our study, we did not find any relevant results indicating that this polymorphism is related to neuromuscular performance in amateur football players. The lack of genetic studies on the _CKM_ gene and its association with muscle damage creates the need for further research to confirm the results found in this study.