# Isolation and Characterization of a Novel Cyanophage Encoding Multiple Auxiliary Metabolic Genes
## 3 Results and Discussion
### Cyanophages Structural Proteome
\begin{table}
\begin{tabular}{c c c c c c c c c} \hline
**Locus Tag** & **Putative Function** & **Mass** & **aa** & **Peotides** & **Coverage** & **Score** & **\(p\)-Value** & **em PAI** \\
**Number** & & **(kDa)** & **Length** & **Peotides** & **Coverage** & **Score** & **\(p\)-Value** & **em PAI** \\ \hline S-SZBM1\_176 & Major capsid protein & 48.46 & 452 & 27 & 42.3\% & 50,627 & 0 & 27.64 \\ \hline S-SZBM1\_180 & \begin{tabular}{c} Portal vertex of the \\ head \\ \end{tabular} & 62.08 & 535 & 19 & 32.5\% & 4979 & 0 & 2.83 \\ \hline S-SZBM1\_195 & Hypothetical protein & 9.06 & 87 & 2 & 43.7\% & 953 & \(5.01\times 10^{-96}\) & 2.66 \\ \hline S-SZBM1\_174 & Tail completion protein & 21.59 & 191 & 5 & 22.0\% & 491 & \(7.94\times 10^{-50}\) & 1.38 \\ \hline S-SZBM1\_204 & \begin{tabular}{c} Baseplate wedge \\ domain-containing \\ structural protein \\ \end{tabular} & 55.49 & 511 & 13 & 31.3\% & 6096 & 0 & 1.37 \\ \hline S-SZBM1\_54 & \begin{tabular}{c} YadA \\ domain-containing \\ structural protein \\ \end{tabular} & 47.55 & 444 & 12 & 35.1\% & 2493 & \(5.01\times 10^{-250}\) & 1.24 \\ \hline S-SZBM1\_53 & Structural protein & 76.76 & 734 & 13 & 27.7\% & 4393 & 0 & 1.21 \\ \hline S-SZBM1\_90 & \begin{tabular}{c} DUF680 \\ domain-containing \\ protein \\ \end{tabular} & 12.58 & 122 & 2 & 25.4\% & 1159 & \(1.26\times 10^{-116}\) & 1.06 \\ \hline S-SZBM1\_203 & \begin{tabular}{c} Baseplate wedge tail \\ fiber connector \\ \end{tabular} & 269.22 & 2446 & 53 & 27.1\% & 17,271 & 0 & 1.03 \\ \hline S-SZBM1\_206 & \begin{tabular}{c} Baseplate wedge \\ protein \\ \end{tabular} & 51.38 & 498 & 9 & 24.9\% & 2195 & \(3.16\times 10^{-220}\) & 0.98 \\ \hline S-SZBM1\_136 & \begin{tabular}{c} Viiron structural \\ protein \\ \end{tabular} & 21.23 & 199 & 2 & 24.1\% & 7040 & 0 & 0.80 \\ \hline S-SZBM1\_74 & \begin{tabular}{c} Baseplate wedge \\ component \\ \end{tabular} & 33.50 & 292 & 6 & 19.9\% & 858 & \(1.58\times 10^{-86}\) & 0.76 \\ \hline S-SZBM1\_182 & Tail sheath protein & 100.56 & 924 & 12 & 17.9\% & 4763 & 0 & 0.72 \\ \hline S-SZBM1\_197 & \begin{tabular}{c} Tail sheath stabilizer \\ and completion protein \\ \end{tabular} & 30.07 & 265 & 4 & 22.3\% & 1414 & \(3.98\times 10^{-142}\) & 0.69 \\ \hline S-SZBM1\_200 & Structural protein & 215.00 & 2073 & 25 & 17.0\% & 10,556 & 0 & 0.67 \\ \hline S-SZBM1\_199 & Neck protein & 31.07 & 264 & 5 & 14.0\% & 541 & \(7.94\times 10^{-55}\) & 0.66 \\ \hline S-SZBM1\_52 & Structural protein & 129.96 & 1192 & 16 & 20.2\% & 5256 & 0 & 0.60 \\ \hline S-SZBM1\_208 & \begin{tabular}{c} Baseplate wedge \\ initiator \\ \end{tabular} & 724.20 & 6619 & 71 & 13.5\% & 11,794 & 0 & 0.41 \\ \hline S-SZBM1\_139 & Hypothetical protein & 48.43 & 457 & 4 & 10.5\% & 2382 & \(6.31\times 10^{-239}\) & 0.39 \\ \hline S-SZBM1\_205 & \begin{tabular}{c} Baseplate wedge \\ initiator \\ \end{tabular} & 65.69 & 615 & 6 & 10.9\% & 1559 & \(1.26\times 10^{-156}\) & 0.34 \\ \hline S-SZBM1\_178 & \begin{tabular}{c} Prohead core \\ scaffolding protein and \\ protease \\ \end{tabular} & 23.91 & 215 & 2 & 21.9\% & 361 & \(7.94\times 10^{-37}\) & 0.30 \\ \hline S-SZBM1\_140 & \begin{tabular}{c} Cytidylyltransferase \\ \end{tabular} & 40.96 & 362 & 2 & 13.5\% & 169 & \(1.26\times 10^{-17}\) & 0.26 \\ \hline S-SZBM1\_209 & \
\begin{tabular}{c} Baseplate wedge \\ subunit \\ \end{tabular} & 76.50 & 691 & 5 & 9.7\% & 507 & \(2.00\times 10^{-51}\) & 0.23 \\ \hline S-SZBM1\_190 &
\begin{tabular}{c} Viiron structural \\ protein \\ \end{tabular} & 46.62 & 424 & 3 & 7.1\% & 461 & \(7.94\times 10^{-47}\) & 0.23 \\ \hline S-SZBM1\_177 & Prohead core protein & 37.27 & 339 & 2 & 19.8\% & 248 & \(1.58\times 10^{-25}\) & 0.19 \\ \hline S-SZBM1\_65 & Hypothetical protein & 60.80 & 536 & 3 & 10.8\% & 183 & \(5.01\times 10^{-19}\) & 0.17 \\ \hline S-SZBM1\_181 & Tail tube protein & 24.75 & 224 & 1 & 7.6\% & 98 & \(1.58\times 10^{-10}\) & 0.14 \\ \hline \end{tabular}
\end{table} 
Table 1: Confirmed proteins encoded by genes in cyanophage S-SZBM1 (score > 50). DUF680 domain-containing protein (PhCOG173) is a conserved cyanophage-specific protein.