When this conglomerate is mixed with an added external carbon source, the growth of heterotrophic bacteria is stimulated and the absorption of nitrogen occurs through the production of microbial proteins, which serve as a food source for fish that is available 24 h per day [6; 7; 8; 9]. Biofloc systems are mainly used to cultivate tilapia (_Oreochromis_ sp.) and white shrimp (_Litopenaeus vannanei_) because both species can eat biofloc and live in environments with high levels of turbidity [10]. The biofloc community can also be used to improve water quality by adding carbon sources to the pond [11; 12]. The development of new technologies and scientific studies in aquaculture is essential to improve intensive fish production. A promising improvement in aquaculture comes from light emitting diode (LED) lighting. It has been shown that lighting in aquaculture can influence embryonic development, releasing reproductive hormones that increase fish growth [13; 14]. However, fish are visual feeders that need a minimum light intensity to eat and, thus, grow and develop [15]. In addition to the influence on embryonic development, the intensity and spectrum light in certain photoperiods (intensity, duration, and periodicity) can be used to alter and control the growth of fish [16; 17; 18; 19; 20]. Photoperiods also influence the release of reproductive hormones, which play an important role in fish reproduction and growth [13]. In addition, under short wavelengths such as blue light, melatonin (which is the hormone that is responsible for sleep) decreases in the bass fish, and the lower the melatonin, the longer the fish are awake, and the more they feed [21]. However, banana shrimp (_Penaus merguiensis_) have a faster growth related to the intensity of the lighting, and the higher the light intensity, the less the shrimp feed, but they grow faster, possibly because of better efficiency of food assimilation [22]. For shrimp with BFT, it was observed that when low light intensity was used, shrimp production decreased by 48%, and the density of microalgae, zooplankton, and rotifers also decreased by 60%, 60%, and 90%, respectively [23]. Tilapias are generally diurnal feeders that feed at different time periods during the day [24]. Using fluorescent tube lamps with a photoperiod of 18L:6D and illuminance of 2500 lux, it is possible to produce more Nile tilapia seeds (percentage of spawning synchrony and percentage of the sac and swim-up fry stages) compared to a shorter photoperiod with less illumination (2500 lux/15 h, 2500 lux/12 h, 500 lux/18 h, 500 lux/15 h, and 500 lux/12 h) [14]. The promising new LED light technology, which has not been widely explored in aquaculture, especially in BFT systems, can be a useful light source tool if the light parameters (intensity, color, and periodicity) are analyzed and applied to obtain benefits in the production of Nile tilapia in BTF. The rapid development of LED technology in recent years has exceeded the characteristics of incandescent lamps in luminous efficiency, low heat emission, robustness, environment resistance, non-toxicity, durability, and adjustable light intensity and wavelength, which allows precise control of the light spectrum [25; 26]. The aim of this study was to investigate the effect of using colored LED light on tilapia growth, the nutritional composition of the Nile tilapia fingerlings, and the composition of the bioflocs that are used as a food source for fish.