## 3 Results
### GC-TOFMS Analysis  
Identification and quantification of metabolites from the roots, stems, and leaves of _L. pubescens_ were carried out. A total of 48 metabolites (one amine, two phenolic acids, two carbohydrate intermediates, four sugar alcohols, four citric acid cycle (CAC) intermediates, seven carbohydrates, 18 amino acids, and 10 organic acids) were detected in the different plant parts of _L. pubescens_ (Figure S4). Among the CAC cycle intermediates, the levels of citric acid, malic acid, and fumaric acid were higher in the leaves and roots of _L. pubescens_. Similarly, organic acid levels were higher in the leaves and roots. Shikimic acid, glyceric acid, and threonine acid levels were higher in the leaves and lactic acid, oxalic acid, and glycolic acid levels were higher in the roots. Pyrvvic acid levels were higher in the leaves and roots. Among the carbohydrates and their derivatives, the levels of fructose, mannose, galactose, glucose, and fructose-6-phosphate, excluding sucrose, were higher in the stems and roots. In contrast, the levels of sugar alcohols were higher in the leaves. For the amino acid biosynthesis, most amino acid levels were higher in leaves and stems. The levels of aspartic acid, glutamic acid, phenylalanine, and tyrosine were higher in leaves and the levels of pyroglutamic acid, 4-aminobutyric acid, asparagine, and glutamine were higher in the stems. PCA supported the above description on metabolite differences in the roots, stems, and leaves of _L. pubescens_. The PCA created a visualization of this metabolite data of the different plant parts of _L. pubescens_ with two components (39.9 and 26.6% of the variance, \begin{table}
\begin{tabular}{c c c} \hline \hline
**Organ** & **Oleanolic Acid** & **Unsolic Acid** \\ \hline Root & 0.107 \(\pm\) 0.00471 c  1 & 0.120 \(\pm\) 0.00100 c  \\ Stem & 1.20 \(\pm\) 0.0464 a  & 1.75 \(\pm\) 0.0535 a  \\ Leaf & 0.963 \(\pm\) 0.00943 b  & 1.62 \(\pm\) 0.0262 b  \\ \hline \hline \end{tabular}
\end{table} 
Table 1: The contents of oleanolic acid and unsolic acid detected in the roots, stems, and leaves of _L. Lavandula pubescens_ (mg/g dry weight). \begin{table}
\begin{tabular}{c c c} \hline \hline
**Organ** & **Total Phenolic Content** & **Total Flavonoid Content** \\  & **(mg GAE/g DW)** & **(mg RE/g DW)** \\ \hline Root & 8.08 \(\pm\) 0.11 b  1 & 27.76 \(\pm\) 0.26 b  \\ Stem & 3.83 \(\pm\) 0.07 c  & 11.71 \(\pm\) 0.18 c  \\ Leaf & 9.58 \(\pm\) 0.19 a  & 29.56 \(\pm\) 0.51 a  \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Total phenolic and total flavonoid content in the roots, stems, and leaves of _L. pubescens_. respectively) in Figure 5A. Specifically, principal component 1 (PC1) displayed that the leaf group was separated from the stem and root groups, but the stem group was not separated from the root group and PC2 indicated a clear separation between the root and stem group. The most important metabolites of PC1 were glyceric acid, phenylalanine, xylitol, shikimic acid, and glutamic acid for which the eigenvector values were \(-0.21639\), \(-0.21438\), \(-0.21136\), \(-0.20955\), and \(-0.19997\), respectively, and glucose, fructose, galactose, glutamine, and asparagine, for which the values were 0.19391, 0.18685, 0.1859, 0.18396, and 0.17692, respectively. These metabolites resolved this separation among the groups. In addition, PLS-DA, used to maximize the separation between groups, exhibited a clear separation between the roots and stems of _L. pubescens_ in its two components, explaining 38.6 and 27.1% of the variance, respectively (Figure 5B). Figure 5: (**A**) scores and loading plots of PCA model and (**B**) scores and loading plots of PLDA model obtained from metabolites detected in different organs of _L. pubescens_ using HPLC and GC-TOFMS. The relationship between the metabolites detected in the roots, stems, and leaves of _L.__pubscens_ was investigated using [PERSON]'s correlation (Figure 6).