## 3 Results
### Antioxidant Activity of Dipteryx punctata Extracts by TLC and DPPH Free Radical Scavenging  
From the screening by TLC, it was found that the extracts from the different parts of the plant showed signs of antioxidant activity given the presence of a whitish color at the sample application points on the plates developed with ABTS (2,2\({}^{\prime}\)-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)) (Figure 4A1-D1), and the presence of a yellowish color in the plates developed with DPPH (2,2-diphenyl-1-picrylhydrazyl) (Figure 4A2-D2). It was also observed that there were no differences in color or intensity between the extracts from the different collection areas; this characteristic was identified in the chromatographic plates that evaluated the classes of compounds (Figure 4). Quantitative analysis using the DPPH radical scavenging method, performed with extracts of leaves, branches, residues, and seeds, containing representatives from all collection areas, corroborated the TLC results, indicating significant neutralization of the radical by the change in color from purple or violet to light yellow or pale violet with the increasing concentrations tested (Figure 5). \begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline  & & \multicolumn{3}{c}{**SEEDS EXTRACS**} & **Area 1** & **Area 2** & **Area 3** & **Area 4** & **Area 5** \\ \hline
**N\({}^{*}\)** & **IRT** & **FRT** & **COMPOUNDS** & **RC \%** & **RC \%** & **RC \%** & **RC \%** & **RC \%** \\ \hline
01 & 3.78 & 3.98 & O-Methylhydroxylamine & - & 1.0 & - & - & - \\
02 & 6.10 & 6.11 & 1-Ethyl-1-methylcyclopropane & - & - & 0.7 & 2.6 & - \\
03 & 25.30 & 25.76 & Hydrocoumarin & 3.3 & - & - & - & - \\
04 & 27.52 & 28.04 & Coumarin & 5.4 & 98.1 & 99.3 & 97.4 & 93.8 \\
05 & 28.86 & 29.09 & 4-Aminopyrido[3,2-c] pyridazine & - & 0.9 & - & - & - \\
06 & 46.43 & 46.72 & n-Hexadecanoic acid (palmitic acid) & 6.1 & - & - & - & - \\
07 & 47.48 & 48.33 & Hexadecanoic acid ethyl ester (palmitic acid ethyl ester) & 4.2 & - & - & - \\
08 & 51.53 & 52.41 & (Z)-11-Octadecenoic acid (cis-vaccenic acid) & 8.5 & - & - & - \\
09 & 52.85 & 53.03 & (Z)-9-Octadecenoic acid (oleic acid) & 32.5 & - & - & - & 6.2 \\
10 & 53.28 & 53.63 & Octadecanoic acid (stearic acid) & 4.3 & - & - & - & - \\
11 & 53.63 & 54.23 & Octadecanoic acid ethyl ester (stearic acid ethyl ester) & 3.5 & - & - & - \\
12 & 57.63 & 58.08 & (Z)-13-Eicosenoic acid (cis-13-eicosenoic acid) & 1.3 & - & - & - & - \\
13 & 58.96 & 59.46 & Eicosanoic acid ethyl ester & 1.3 & - & - & - & - \\
14 & 61.93 & 62.63 & Hexadecanoic acid 2-hydroxy-1-hydroxymethyl ethyl ester & 1.4 & - & - & - & - \\  & & & & & & & & \\
15 & 62.63 & 63.26 & Bis (2-ethylhexyl)phthalate & 1.4 & - & - & - & - \\
16 & 63.26 & 64.18 & Docosanoic acid & 2.7 & - & - & - & - \\
17 & 64.18 & 64.63 & Docosanoic acid & 4.1 & - & - & - & - \\
18 & 66.03 & 67.21 & (Z)-9-Octadecenoic acid 2.3-dihydroxypropyl ester & 11.0 & - & - & - & - \\
19 & 67.21 & 67.56 & Octadecanoic acid 2,3-dihydroxypropyl ester & 1.0 & - & - & - & - \\
20 & 68.33 & 68.86 & Tetracosaonic acid & 1.0 & - & - & - & - \\
21 & 68.86 & 69.38 & Ethly tetradesanoate & 3.3 & - & - & - & - \\
22 & 78.48 & 78.96 & Stigmasterol & 1.4 & - & - & - & - \\
23 & 79.81 & 80.38 & \(\gamma\)-Sitosterol & 2.1 & - & - & - & - \\ Total \% & & & 100 & 100 & 100 & 100 & 100 \\ \hline \hline \end{tabular} Note: IRT = initial retention time; FRT = final retention time of the compounds obtained in the collection areas; RC % = relative content in percentage; Appendix A (Figures A16–A20) = chromatograms of the seed extracts in the five collection areas.  
\end{table} 
Table 7: Chemical compounds identified in ethanolic extracts of the seeds of _Dipteryx punctata_, in the five collection areas of the municipality of Mojui dos Campos, Eastern Amazon, Brazil. The logarithmic function adequately represents the consumption of DPPH as a function of the increase in concentrations in this study, in the three phases: the first characterized  
Figure 4: Antioxidant screening of _Dipteryx punctata_ extracts.