## 3 Results
### The Effect of Different Hormones on the Color and Quality Traits of Grape Berry
#### 3.1.4 Effects of Different Hormones on Grape Volatile Compound  
The volatile compound identification and relative contents were based on data including the retention time, BP area, and BP height as obtained from GC-MS (Figure S2 and Table S6). A variety of volatile components were detected in grape flesh, mainly including acids, alcohols, esters, terpen, aldehydes, ketones, and phenols, among which the contents of alcohol substances were the highest, followed by aldehydes. From the qualitative and quantitative analyses, there were some differences in the types and relative contents \begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Physiological**} & \multicolumn{5}{c}{**Different Treatment Periods**} \\ \cline{2-6}  & **Treatment** & **7 DAT** & **14 DAT** & **21 DAT** & **28 DAT** & **35 DAT** \\ \hline \multirow{6}{*}{berry weigh (g)} & CK & 6.13 \(\pm\) 0.16  & 8.06 \(\pm\) 0.09  & 8.31 \(\pm\) 0.15  & 8.53 \(\pm\) 0.14  \\ \cline{2-6}  & GA & 6.05 \(\pm\) 0.16  & 6.56 \(\pm\) 0.22  & 7.78 \(\pm\) 0.35  & 8.23 \(\pm\) 0.11  & 8.38 \(\pm\) 0.19  \\ \cline{2-6}  & ABA & 6.23 \(\pm\) 0.19  & 7.72 \(\pm\) 0.21  & 8.39 \(\pm\) 0.38  & 8.50 \(\pm\) 0.22  & 8.92 \(\pm\) 0.29  \\ \cline{2-6}  & MeJA & 6.19 \(\pm\) 0.12  & 6.97 \(\pm\) 0.23  & 8.18 \(\pm\) 0.23  & 8.41 \(\pm\) 0.27  & 8.61 \(\pm\) 0.26  \\ \cline{2-6}  & NAA & 6.57 \(\pm\) 0.15  & 7.32 \(\pm\) 0.17  & 7.84 \(\pm\) 0.14  & 7.97 \(\pm\) 0.13  \\ \hline \multirow{6}{*}{soluble solid (\%)} & CK & 12.57 \(\pm\) 0.22  & 15.61 \(\pm\) 0.21  & 17.05 \(\pm\) 0.39  & 18.12 \(\pm\) 0.47  & 21.21 \(\pm\) 0.73  \\ \cline{2-6}  & GA & 12.25 \(\pm\) 0.37  & 12.57 \(\pm\) 0.22  & 15.41 \(\pm\) 0.21  & 17.58 \(\pm\) 0.39  & 19.09 \(\pm\) 0.61  \\ \cline{2-6}  & ABA & 14.63 \(\pm\) 0.41  & 16.46 \(\pm\) 0.52  & 18.81 \(\pm\) 0.52  & 20.13 \(\pm\) 0.52  & 22.71 \(\pm\) 0.67  \\ \cline{2-6}  & MeJA & 13.84 \(\pm\) 0.52  & 15.89 \(\pm\) 0.34  & 17.93 \(\pm\) 0.62  & 19.44 \(\pm\) 0.69  & 21.82 \(\pm\) 0.59  \\ \cline{2-6}  & NAA & 11.39 \(\pm\) 0.23  & 12.46 \(\pm\) 0.31  & 14.82 \(\pm\) 0.39  & 16.37 \(\pm\) 0.43  & 17.54 \(\pm\) 0.41  \\ \hline \end{tabular}
\end{table} 
Table 1: _Cont._of volatile compounds treated with different hormones. In the control samples (CK), 2-hexenal, hexanal, and 3-hexen-1-OL (Z)- accounted for 31.59%, 9.80%, and 9.22% of the total volatiles, respectively. In GA treated samples, 2-hexenal, henzyl 2-chloroethyl sulfone, and cyclopropane, (1-methylethylene)- accounted for 33.35%, 20.37%, and 12.56% of the total volatiles, respectively, while 2-hexenal, hexanal, and 3-hexen-1-ol accounted for 40.93%, 11.44%, and 10.47% in the ABA treated samples. In the MeJA treated samples, the three highest volatile substances were 2-hexenal, 1, 3, 5-cycloheptatriene, and trans-2-hexenol, accounting for 30.91%, 7.61%, and 5.96% of the total volatile substances respectively, while 2-hexenal, 3-hexen-1-ol, (Z)-, and formamide, N,N-dibutyl- accounted for 9.97%, 6.98%, and 6.70% of total volatiles, respectively, in the NAA treatment samples. Among the five samples, the total amount of volatile compounds in the MeJA treatment samples was the highest, followed by ABA and GA, while the total amount of volatile compounds in the CK and NAA treatment samples were the lowest. Compared with the control group (CK), the total amount of volatile compounds in grape berries treated with MeJA and ABA increased by 81.97% and 28.69%, respectively. Cis-element Analysis and GUS Activity Detection of VvMIR156b/c/d Promoters in Response to Exogenous Hormones  
As shown in Figure 2 and Table S7, promoter cis-elements of VvMIR156b/c/d can be classified as light-related, hormone-related, stress-related, tissue-specific, and circadian-related elements based on their potential functions.