## 3 Results
### Qualitative Variables
#### 3.2.3 Self-Assessment  
The questionnaire fulfilled by subjects reflected that at the end of the study, up to 97% of the subjects would recommend the treatment regimen and informed that the protocol regimen met their expectations. All of them found the texture of the products pleasant and the daily protocol easy to follow. As for the questions about the efficacy of the treatment, 92.5% had positive answers. All the results discussed above were in accordance with the improvements shown in the digital pictures (Figure 8):  
Figure 7: Values describe the percentages of subjects who had improvements and those who did not based on the RAO Goldman scale versus the baseline. The asterisks signal a significant difference in 52% of the subjects at T28 and 69% of the subjects at T42 versus baseline. * \(p<0.05\). An individualized anti-aging treatment regimen for SS subjects should be prescribed in order to increase tolerance and subsequently compliance and efficacy. This represents a therapeutic challenge since some of the anti-aging active ingredients with more demonstrated scientific evidence (e.g., retinoids) can induce a certain degree of skin irritation that would worsen the symptoms of subjects with SS [1]. Results obtained after 42 days in an in vivo study following the regimen combination showed an improvement in SS conditions. We present an anti-aging treatment regimen combining actives with published evidence of anti-inflammatory and barrier protection actions (Aquammunist(r) and niacinamide), neurosensory function protection (_Annuona chernola_ Fruit Extract) plus anti-aging activity based on a TGFb2 peptide from the _N. benthamiana_ plant focused on extracellular matrix (ECM) regeneration [16; 20; 26; 27; 31; 37]. The results showed a significant improvement in skin aging parameters, such as firmness and elasticity at T14 and wrinkle depth at T28, evaluated by quantitative analysis and in accordance with wrinkle appearance referred by investigators and subjects. We hypothesize that this anti-aging effect is attributable to the synergistic mechanisms of the action of all the actives included in the cosmetic regimen. As the levels and activity of TGFb decrease within the aging process and affect the components of the ECM, actives that regulate the synthesis of ECM proteins are especially useful. It is noteworthy that the TGFb2 peptide present in the serum is a plant-derived TGFb2 with proven activity in increasing epidermal thickness and improving neutral lipid levels in in vitro studies. In clinical trials, TGFb2 showed an increase in skin elasticity, firmness, thickness, radiance and hydration, and reduced size, volume and depth of wrinkles without photoxicity [32]. In addition, some studies reported that TRPV1 and Ca2+ regulate the expression and activation of matrix metalloproteinases (MMP-1) in UV-induced photogaling, leading to ECM degradation, wrinkle formation, laxity and skin fragility [39]. [PERSON] et al. suggested that the development of TRPV1-specific non-toxic inhibitors may be an effective strategy for the prevention of skin aging [39]. _Anmonia chernola_ fruit extract acts as a down-regulator of TRPV1 [16; 31], and although its exact implications on skin aging have yet to be determined, it represents a cornerstone of treatment in the pathogenesis of sensitive skin, where neurogenic hyperreactivity is part of the process. As for the parameters related to SS and its symptoms, a significant improvement in erythema and moisturization was obtained from T14 onwards, and a significant improvement in skin barrier function (<TEWL) was assessed at T28. Therefore, this sensitive skin treatment regimen was shown to induce greater skin resistance with a decrease in sensitivity and reactivity. We hypothesized a synergistic effect of the active ingredients present in the regimen [40]. Niacinamide could contribute to the improvement of these parameters due to its potential action on skin barrier function protection and decrease in coloration by inhibiting transference of melanin and antioxidative effects [27; 28].