## 3 Results and Discussion
atrense_ is an omnivorous species that feeds in different habitats, so its diet does not depend exclusively on the seabed [30]. \begin{table}
\begin{tabular}{c c c c c c c} \hline  & \multicolumn{4}{c}{_Serranus atricauda_} & \multicolumn{4}{c}{_Sparisoma cretense_} \\ \hline  & **North** & **East** & **South** & **North** & **East** & **South** \\ \hline Cr Liver & \(<\)LD & \(<\)LD & \(<\)LD & \(<\)LD & \(<\)LD & \(<\)LD \\ Cu Muscle & \(0.549\pm 0.142\) & \(0.518\pm 0.149\) & \(0.61\pm 0.11\) & \(0.61\pm 0.251\) & \(0.408\pm 0.086\) & \(0.419\pm 0.097\) \\ Cu Liver & \(4.637\pm 4.501\) & \(4.804\pm 3.066\) & \(3.911\pm 1.906\) & \(5.083\pm 2.485\) & \(7.012\pm 3.078\) & \(1.759\pm 0.812\) \\ Fe Muscle & \(2.946\pm 2.532\) & \(2.583\pm 0.615\) & \(3.447\pm 1.032\) & \(2.675\pm 1.369\) & \(2.201\pm 0.899\) & \(2.78\pm 1.567\) \\ Fe Liver & \(124.1\pm 138.47\) & \(145.2\pm 197.5\) & \(129\pm 92.3\) & \(133.2\pm 47.512\) & \(100.9\pm 140.8\) & \(27.8\pm 16.66\) \\ K Muscle & \(1860\pm 488\) & \(2462\pm 319\) & \(2501\pm 112\) & \(2357\pm 912.6\) & \(2222\pm 63.5\) & \(2564\pm 239.7\) \\ K Liver & \(2127\pm 870.2\) & \(3508\pm 3048\) & \(3486\pm 1931\) & \(3507\pm 1400\) & \(3129\pm 668.3\) & \(874.3\pm 467.2\) \\ Li Muscle & \(0.186\pm 0.105\) & \(0.265\pm 0.122\) & \(0.272\pm 0.1\) & \(0.308\pm 0.24\) & \(0.191\pm 0.087\) & \(0.225\pm 0.129\) \\ Li Liver & \(3.675\pm 3.32\) & \(3.793\pm 4.742\) & \(2.398\pm 1.251\) & \(2.678\pm 2.427\) & \(4.918\pm 5.362\) & \(2.72\pm 2.086\) \\ Mg Muscle & \(396.9\pm 157.4\) & \(328.3\pm 67.58\) & \(333.3\pm 23.05\) & \(323.3\pm 177\) & \(289\pm 20.4\) & \(387.8\pm 187.7\) \\ Mg Liver & \(536.8\pm 587\) & \(340\pm 298.9\) & \(345.7\pm 154\) & \(720.2\pm 749.8\) & \(249.9\pm 139.2\) & \(157\pm 95.52\) \\ Mn Muscle & \(0.272\pm 0.257\) & \(0.245\pm 0.13\) & \(67.84\pm 144.2\) & \(0.724\pm 0.459\) & \(69.69\pm 217.6\) & \(0.576\pm 0.36\) \\ Mn Liver & \(0.8\pm 0.399\) & \(2.033\pm 2.114\) & \(1.569\pm 0.88\) & \(2.815\pm 2.893\) & \(1.749\pm 1.434\) & \(0.411\pm 0.38\) \\ Mo Muscle & \(0.013\pm 0.003\) & \(0.014\pm 0.002\) & \(0.014\pm 0.002\) & \(0.013\pm 0.006\) & \(0.015\pm 0.003\) & \(0.013\pm 0.003\) \\ Mo Liver & \(0.158\pm 0.082\) & \(0.207\pm 0.203\) & \(0.239\pm 0.119\) & \(0.263\pm 0.154\) & \(0.254\pm 0.128\) & \(0.12\pm 0.085\) \\ Na Muscle & \(936.5\pm 355.5\) & \(758.5\pm 153.1\) & \(893.1\pm 125.1\) & \(965.8\pm 427.2\) & \(701.3\pm 60.6\) & \(825.9\pm 271.5\) \\ Na Liver & \(1651\pm 804.5\) & \(1724\pm 845.3\) & \(2008\pm 844.4\) & \(2872\pm 1022\) & \(1887\pm 509.2\) & \(712.3\pm 327.8\) \\ Ni Muscle & \(0.029\pm 0.016\) & \(0.025\pm 0.016\) & \(0.021\pm 0.011\) & \(0.036\pm 0.034\) & \(0.043\pm 0.05\) & \(0.015\pm 0.013\) \\ Ni Liver & \(0.206\pm 0.26\) & \(0.372\pm 0.886\) & \(0.061\pm 0.088\) & \(0.336\pm 0.171\) & \(0.438\pm 0.233\) & \(0.126\pm 0.099\) \\ Pb Muscle & \(0.036\pm 0.046\) & \(0.042\pm 0.049\) & \(0.018\pm 0.003\) & \(0.022\pm 0.01\) & \(0.02\pm 0.004\) & \(0.021\pm 0.009\) \\ Pb Liver & \(0.185\pm 0.139\) & \(0.158\pm 0.125\) & \(0.098\pm 0.074\) & \(0.168\pm 0.091\) & \(0.497\pm 0.462\) & \(0.047\pm 0.024\) \\ Zn Muscle & \(3.951\pm 0.618\) & \(4.499\pm 0.705\) & \(4.007\pm 0.212\) & \(2.87\pm 1.111\) & \(3.434\pm 0.334\) & \(3.079\pm 0.281\) \\ Zn Liver & \(22.883\pm 6.919\) & \(30.48\pm 20.91\) & \(29.59\pm 12.44\) & \(22.5\pm 8.67\) & \(20.05\pm 14.58\) & \(9.134\pm 5.123\) \\ \hline \end{tabular}
\end{table} 
Table 3: Results of the two-way PERMANOVA analyzing the variation in the content of heavy metals and trace elements in muscle tissue in species according to study zone, considering the level of the factor “Species”. The PERMANOVA analysis showed significant differences in the content of metals and trace elements in the liver in _S. cretense_ in the southern zone with respect to the northern and eastern zones (Table 6). The analysis of the pairwise comparisons between the locations for each species shows that all metals and trace elements, with the exception of Li, have statistically significant differences in the case of _S.