## 2 Results
### Fatty Acid Composition
#### 2.1.2 Cow Milk  
The fatty acid composition of milk obtained from cows fed mycorrhizal (S + m) and nonmycorrhizal (S) sorghum silage is reported in Table 2. Butyric acid (C4:0) and hexanoic acid (C6:0) showed significant increases of 39% and 28%, respectively, in S + m over S milk samples. On the contrary, MUFAs in cow milk showed a significant reduction only for nervonic acid (C14:1) of about 51%. n-3 and n-6 PUFAs did not show significant changes, except for docosahexaenoicenoic acid (DHA) (C22:6n3), which decreased by  
\begin{table}
\begin{tabular}{c c c c c c c} \hline  & **Fatty Acids** & **M** & **sd** & **M + m** & **sd** & **Change (\%) \({}^{\circ}\) M + m/M** & \\ \hline C4:0 & Butyric acid & 7.41 & 0.27 & 9.00 & 0.59 & +22 & * \\ C6:0 & Caproic acid & 3.64 & 0.07 & 4.77 & 0.45 & +31 & * \\ C8:0 & Caprylic acid & 1.91 & 0.26 & 2.21 & 0.19 & +16 & * \\ C10:0 & Capric acid & 3.15 & 0.27 & 3.91 & 0.19 & +24 & * \\ C11:0 & Undecanoic acid & 0.07 & 0.01 & 0.07 & 0.01 & +3 & \\ C12:0 & Lauric acid & 3.74 & 0.26 & 4.37 & 0.325 & +17 & \\ C13:0 & Trideylic acid & 0.16 & 0.01 & 0.15 & 0.01 & \(-8\) & \\ C14:0 & Myristic acid & 14.11 & 0.39 & 14.82 & 0.035 & +5 & \\ C15:0 & Pentadecylic acid & 1.50 & 0.08 & 1.37 & 0.02 & \(-9\) & \\ C16:0 & Palmitic acid & 39.49 & 1.23 & 37.94 & 0.09 & \(-4\) & \\ C17:0 & Margaric acid & 0.49 & 0.01 & 0.39 & 0.03 & \(-20\) & * \\ C18:0 & Stearic acid & 5.92 & 0.48 & 4.72 & 0.72 & \(-20\) & \\ C24:0 & Lignoercic acid & 0.05 & 0.01 & 0.05 & 0.00 & \(-16\) & \\
**SFA** & & **81.64** & **0.15** & **83.8** & **1.02** & +3 & * \\ C14:1n9 & Myristoleic acid & 1.60 & 0.13 & 1.70 & 0.00 & +6 & \\ C16:1n9 & Palmitoleic acid & 2.73 & 0.36 & 2.76 & 0.11 & +1 & * \\ C17:1 & Heptadecenoic acid & 0.29 & 0.00 & 0.26 & 0.00 & \(-12\) & * \\ C18:1n9t & Elaidic acid & 0.25 & 0.01 & 0.38 & 0.19 & +53 & \\ C18:1n9c & Oleic acid & 11.37 & 0.73 & 9.31 & 1.05 & \(-18\) & * \\ C18:1n7 & Vaccemic acid & 0.16 & 0.01 & 0.12 & 0.02 & \(-21\) & \\ C20:1n9 & Eicosenoic acid & 0.08 & 0.0 & 0.07 & 0.01 & \(-15\) & \\ C22:1n9 & Erucic acid & 0.03 & 0.0 & 0.03 & 0.01 & +6 & \\ C24:1 & Nervonic acid & 0.06 & 0.01 & 0.05 & 0.0 & \(-26\) & * \\
**MUFA** & & **16.5** & **0.22** & **14.68** & **0.79** & \(-11\) & * \\ C18:2n6 tc+ct & Trans-linoleic acid & 0.21 & 0.02 & 0.14 & 0.01 & \(-32\) & * \\ C18:2n6c & Linoleic acid & 1.10 & 0.03 & 0.90 & 0.14 & \(-19\) & \\ CLA & Conjugated linoleic acid & 0.31 & 0.02 & 0.25 & 0.04 & \(-17\) & \\ C20:3n6 & Homo-\(\gamma\)-linoleic acid & 0.04 & 0.00 & 0.04 & 0.01 & \(-3\) & \\ C20:4n6 & Arachidonic acid & 0.10 & 0.01 & 0.10 & 0.02 & +6 & \\ C22:2n6 & Docosadienoic acid & 0.04 & 0.01 & 0.05 & 0.00 & +4 & \\
**PUFA n-6** & & **1.59** & **0.06** & **1.34** & **0.24** & \(-16\) & \\ C18:3n3 & \(\alpha\)-Linolenic acid & 0.13 & 0.00 & 0.10 & 0.01 & \(-24\) & * \\ C20:5n3 & Eicosapentaenoic acid (EPA) & 0.02 & 0.00 & 0.02 & 0.00 & +1 & \\ C22:6n3 & Docosahexaenoic acid (DHA) & 0.04 & 0.01 & 0.03 & 0.00 & \(-16\) & \\
**PUFA n-3** & & **0.19** & **0.01** & **0.15** & **0.02** & \(-19\) & * \\
**av6/a3** & & **8.42** & **0.15** & **8.74** & **0.67** & +4 & \\ \hline \end{tabular}
\begin{tabular}{c c c c c c} \hline \multicolumn{4}{c}{Asterisks indicate significantly different values between milk samples obtained from buffalo fed on mycorrhizal and} \\ (S) sorghum silage is reported in Table 2. Butyric acid (C4:0) and hexanoic acid (C6:0) showed significant increases of 39% and 28%, respectively, in S + m over S milk samples. On the contrary, MUFAs in cow milk showed a significant reduction only for nervonic acid (C14:1) of about 51%. n-3 and n-6 PUFAs did not show significant changes, except for docosahexaenoic acid (DHA) (C22:6n3), which decreased by 39%.  
about 44%. The \(\omega\)-6/\(\omega\)-3 ratio was higher in the M + m milk samples (+27%), although this difference was not statistically significant.