## 4 Discussion  
The results that were obtained indicated that all observed parameters (body weight and length of yellow mealworm larvae and their DM, ash, fat, and crude protein contents) were affected by the rearing temperature. The optimal temperature range for rearing _T. molitor_ is 22-30 \({}^{\circ}\)C [13; 14; 15; 16; 17; 18; 23; 24], which includes the temperatures selected for this experiment. The temperature significantly influenced the growth of larvae. Mealworms reared at 28 \({}^{\circ}\)C grew faster than those reared at 22 \({}^{\circ}\)C. This relationship between insect growth and temperature has been well described for many insects pests. An increase in temperature increases physiological activity, the metabolic rate, the efficiency of energy assimilation, and protein and lipid contents [10]. Thus, insects consume more substrate and grow faster than at lower temperatures [25]. In the case of mealworms, the positive effects of rising \begin{table}
\begin{tabular}{c c c c} \hline
**Amino Acid** & **22 \({}^{\circ}\)C** & **25 \({}^{\circ}\)C** & **28 \({}^{\circ}\)C** \\ \hline Aspartic acid & 3.395 \(\pm\) 0.142 \({}^{\mathrm{a}}\) & 3.375 \(\pm\) 0.111 \({}^{\mathrm{a}}\) & 3.030 \(\pm\) 0.180 \({}^{\mathrm{a}}\) \\ Threonine & 1.962 \(\pm\) 0.073 \({}^{\mathrm{a}}\) & 2.128 \(\pm\) 0.229 \({}^{\mathrm{a}}\) & 1.907 \(\pm\) 0.025 \({}^{\mathrm{a}}\) \\ Serine & 2.208 \(\pm\) 0.106 \({}^{\mathrm{ab}}\) & 2.425 \(\pm\) 0.250 \({}^{\mathrm{a}}\) & 1.940 \(\pm\) 0.041 \({}^{\mathrm{b}}\) \\ Glutamic acid & 3.332 \(\pm\) 0.370 \({}^{\mathrm{ab}}\) & 3.538 \(\pm\) 0.311 \({}^{\mathrm{a}}\) & 2.647 \(\pm\) 0.090 \({}^{\mathrm{b}}\) \\ Proline & 3.343 \(\pm\) 0.031 \({}^{\mathrm{ab}}\) & 3.747 \(\pm\) 0.308 \({}^{\mathrm{a}}\) & 3.040 \(\pm\) 0.064 \({}^{\mathrm{b}}\) \\ Glycine & 2.990 \(\pm\) 0.062 \({}^{\mathrm{a}}\) & 2.693 \(\pm\) 0.263 \({}^{\mathrm{a}}\) & 2.617 \(\pm\) 0.050 \({}^{\mathrm{a}}\) \\ Alanine & 3.568 \(\pm\) 0.056 \({}^{\mathrm{a}}\) & 3.638 \(\pm\) 0.213 \({}^{\mathrm{a}}\) & 2.523 \(\pm\) 0.058 \({}^{\mathrm{b}}\) \\ Valine & 2.388 \(\pm\) 0.035 \({}^{\mathrm{a}}\) & 2.415 \(\pm\) 0.212 \({}^{\mathrm{a}}\) & 2.417 \(\pm\) 0.176 \({}^{\mathrm{a}}\) \\ Isoleucine & 1.538 \(\pm\) 0.040 \({}^{\mathrm{ab}}\) & 1.790 \(\pm\) 0.193 \({}^{\mathrm{a}}\) & 1.453 \(\pm\) 0.063 \({}^{\mathrm{b}}\) \\ Leucine & 2.000 \(\pm\) 0.050 \({}^{\mathrm{a}}\) & 2.090 \(\pm\) 0.229 \({}^{\mathrm{a}}\) & 1.820 \(\pm\) 0.093 \({}^{\mathrm{a}}\) \\ Tyrosine & 1.768 \(\pm\) 0.097 \({}^{\mathrm{ab}}\) & 1.845 \(\pm\) 0.100 \({}^{\mathrm{a}}\) & 1.583 \(\pm\) 0.061 \({}^{\mathrm{b}}\) \\ Phenylalanine & 1.143 \(\pm\) 0.078 \({}^{\mathrm{a}}\) & 1.075 \(\pm\) 0.120 \({}^{\mathrm{a}}\) & 1.107 \(\pm\) 0.041 \({}^{\mathrm{a}}\) \\ Histidine & 1.055 \(\pm\) 0.034 \({}^{\mathrm{a}}\) & 1.153 \(\pm\) 0.164 \({}^{\mathrm{a}}\) & 0.873 \(\pm\) 0.062 \({}^{\mathrm{a}}\) \\ Lysine & 1.635 \(\pm\) 0.030 \({}^{\mathrm{a}}\) & 1.810 \(\pm\) 0.068 \({}^{\mathrm{b}}\) & 1.177 \(\pm\) 0.033 \({}^{\mathrm{c}}\) \\ Arginine & 1.668 \(\pm\) 0.049 \({}^{\mathrm{ab}}\) & 1.757 \(\pm\) 0.197 \({}^{\mathrm{a}}\) & 1.330 \(\pm\) 0.022 \({}^{\mathrm{b}}\) \\ Cysteine & 0.123 \(\pm\) 0.004 \({}^{\mathrm{a}}\) & 0.248 \(\pm\) 0.068 \({}^{\mathrm{b}}\) & 0.197 \(\pm\) 0.037 \({}^{\mathrm{ab}}\) \\ Methionine & 0.445 \(\pm\) 0.018 \({}^{\mathrm{a}}\) & 0.558 \(\pm\) 0.080 \({}^{\mathrm{a}}\) & 0.470 \(\pm\) 0.051 \({}^{\mathrm{a}}\) \\ \hline \end{tabular} Superscripts on the same row represent a significant difference (\(p\leq 0.05\)).  
\end{table} 
Table 2: Amino acid content in g per 100 g DM of _T. molitor_ larvae reared at 22, 25, and 28 \({}^{\circ}\)C.