## 3 Results and Discussion
### Microbiological Air Pollution, Moisture Content of Woodent Walls and Air Humidity  
Table 2 shows the results of the quantitative studies, and Table 3 shows the results of the qualitative studies of the measurements of the microbial contamination of the air. \begin{table}
\begin{tabular}{l c c c c} \hline \hline
**Sampling Location** & **Molds \({}^{*}\) [cfu/m\({}^{3}\)]** & **Bacteria \({}^{*}\) [cfu/m\({}^{3}\)]** & **Impact I/O** & **Molds** & **Bacteria** \\ \hline \hline Nave—southwest corner & 3230 & 140 & 3.98 & 0.77 \\ Nave—southeast corner & 2180 & 140 & 2.69 & 0.77 \\ Nave—northeast corner & 4390 & 210 & 5.42 & 1.67 \\ Nave—northwest corner & 3150 & 110 & 3.88 & 0.6 \\ Ambient air (outside the church) & 810 & 180 & - & - \\ \hline \hline \end{tabular} \({}^{*}\)Recommended permissible air pollution by mold and bacteria (total) in museum rooms—safe for humans: 2500 cfu/m\({}^{3}\), and safe for collections: 150 cfu/m\({}^{3}\).  
\end{table} 
Table 2: Assessment of the mold and bacterial contamination in the air—quantitative analysis. \begin{table}
\begin{tabular}{l c} \hline \hline
**Sampling Location** & **Tagged Mold Fungi** \\ \hline \hline Nave—southwest corner & _Penicillium_ sp. (BSL-1), _Trichoderma viride \({}^{*}\)_ (BSL-1) \\ Nave—southeast corner & _Acemenoinium_ sp. \({}^{*}\) (BSL-1 or 2), _Mucor_ sp. (BSL-1), _T. viride_ (BSL-1) \\ Nave—northeast corner & _Cladosporium herbarum \({}^{*}\)_ (BSL-1), _Penicillium_ sp. (BSL-1), _T. viride_ (BSL-1) \\ Nave—northwest corner & _Acemenoinium_ sp. (BSL-1 or 2), _Mucor_ sp. (BSL-1), _T. viride_ \({}^{*}\) (BSL-1 or 2) \\ \hline \hline \end{tabular} \({}^{*}\)The most abundant species in the samples.  
\end{table} 
Table 3: Assessment of the mold contamination of the air—qualitative analysis. The amount of microorganisms found in the air was lower than the permissible air pollution in public buildings proposed by the Interdepartmental Commission for Maximum Admissible Concentrations and Intensities for Agents Harmful to Health in the Working Environment [16]. An auxiliary criterion for determining whether a building has a source of microbial contamination is the I/O (indoor/outdoor) factor [17]. I/O ratios between 1.5 and 2 indicate an indoor environment with normal conditions, while I/O ratios higher than 2 indicate inappropriate indoor environmental conditions, resulting in intense mold and/or bacterial growth in the tested room [18]. To calculate the above factor, it is assumed that the permissible ambient air pollution (outdoor) is 5000 cfu/m\({}^{3}\)[19]. As a result of this study, the air in the naive of the church was found to contain mold many times higher than in the ambient air (I/O > 2). Among the molds found on the samples collected were allergenic and potentially pathogenic species. It is important to note that molds generally fall under the least dangerous category for humans, according to the biosafety level (BSL), which is a system that ranks the relative danger of various microorganisms. The air microbiome is believed to significantly contribute to the deterioration of historical objects [20]. It is exciting to note that despite the severe dampness and degradation of the wooden walls caused by _Serpula lacrymans_, no intensive development of mold colonies was observed on the wall polychromes. Mold colonies were also not found on the temple furnishings, i.e., high and side altars, oil paintings on canvas, sculptures, professionals, and people's benches. However, numerous mold colonies were found on the surfaces of decorations that were deposited in the side rooms of the tower, where the ventilation and air circulation are severely restricted. In Poland, due to the lack of current legal and normative acts defining the threshold values for air pollution of residential, non-industrial premises, etc., the most commonly cited proposal is the Interdepartmental Commission for Maximum Admissible Concentrations and Intensities for Agents Harmful to Health in the Working Environment.