## 3 Results and Discussion
### In Vitro Antifungal Assays
The  
Figure 6: _Talarmyces coalescens_ average growth curves over 42 days after exposure to the volatilized fraction of the different essential oils. Concentration is given in \(\mu\)L cm\({}^{-3}\). The control group (Concentration 0.0) was shared across all treatments. The shaded area represents the Min–Max range of results per tested concentration. For each EO, data (n = 3) associated with different letters represent significantly different results at the end of the experiment, in accordance with [PERSON]’s post hoc test, \(p<0.05\). remaining EOs tested were unable to inhibit _A. pseudoglaucus_ even at the highest tested concentration, although some significantly impacted its growth, _T. mastichina_ in particular, or at least delayed it, as was the case for _T. villosa_ at 2 \(\mu\)L cm\({}^{-3}\). _Penicillium brevicompactum_ (Figure 4) proved more resilient than both _Aspergillus_, with even _C. citratus_, the best performing EO, only showing complete fungal inhibition at the two highest concentrations tested, and although 0.5 and 1 \(\mu\)L cm\({}^{-3}\) of EO delayed colony inception, statistical analysis showed no significant differences between these  
Figure 7: _Trichoderma longibrachatum_ average growth curves (n = 3) over 42 days after exposure to the volatilized fraction of the different essential oils. Concentration is given in \(\mu\)L cm\({}^{-3}\). The control group (Concentration 0.0) was shared across all treatments. The shaded area represents the Min–Max range of results per tested concentration. For each EO, data (n = 3) associated with different letters represent significantly different results at the end of the experiment, in accordance with [PERSON]’s post hoc test, \(p\) < 0.05.  
concentrations and the control at the end of the experiment. Again, _T. villosa, T. summifera, T. mastichina_ and _A. feniculum_ showed similar growth curve trends to that of the control group, and although _M. pulgeium, M. suaveelens_ and _L. angustifolia_ EO significantly affected fungal growth at higher concentrations, it was not to the extent observed for the same EOs in other fungi species. A somewhat similar outcome was observed in the _T. coalescens_ assays (Figure 5). Again, colonies exposed to _T. villosa_ and _T. summifera_ volatilized EOs displayed similar results to those observed in the control group, and while a EO concentration-dependent delay in colony establishment and growth can be observed for _M. pulgeium, T. mastichina, A. feniculum, M. suaveelens_ and _L. angustifolia_, only the latter two EOs were able to completely inhibit fungal growth: the former only at 2 \(\mu\)L cm\({}^{-3}\), while the latter displayed this ability for concentrations above 1 \(\mu\)L cm\({}^{-3}\). _Cymbopogon citratus_ was once again the best performing EO, with no fungal growth observed at any of the tested concentrations. Finally, for _T. longibrachitatum_ (Figure 6), neither _T. villosa, T. summifera, T. mastichina_ or _A. feniculum_ displayed any ability to inhibit the growth of this species. Once again, _C. citratus_ was the best performing EO, with fungal growth only observed at the lowest tested concentration. _Mentha suaveelens, M. pulgeium_ and _L. angustifolia_ showed varied degrees of success, with both _Mentha_ EOs presenting concentration-dependent partial inhibition of colony growth and _L. angustifolia_ completely inhibiting it at 2 \(\mu\)L cm\({}^{-3}\). It is of relevance to note that in the case of _A. pseudoglaucus, T. coalescens_ and _T. longibrachitatum_, the colony diameter of the control group and some of the treatments reached the edges of the Petri dish (5.5 cm) after 28, 14 and 7 days, respectively. Thus, it was impossible to assess the full extent of inhibition for those EOs that showed partial inhibition. Nevertheless, the aim of this work was to find EOs that were capable of fully inhibiting fungal colony establishment and growth, and to that end, this experimental design limitation was irrelevant. Considering all five tested fungi species, _C.