# Steering the Clinical Translation of Delivery Systems for Drugs and Health Products
## 3 Poster Presentations
### In Vivo Evidence for Novel Brain Targeted Peptide-Conjugated Nanomedicines
In the aim to control and treat DFUs, wound dressings able to absorb exudate, to prevent infections and to promote wound healing, are needed [89]. Chitosan (Chi)and Carboxymethylcellulose (CMC) are most common biodegradable and biocompatible polymers which could be used for the development of different modern wound dressings, either alone or as a part of multilayered wound dressings [90]. Several studies reported that silver nanoparticles (AgNPs) contribute to the wound healing process due to their antioxidant, antimicrobial and anti-inflammatory activity and to their simple incorporation in cotton fabric and dressings [91]. The aim of this work was to develop a biodegradable and biocompatible hydrogel composed by Chi and CMC (HyDrO-DiAb) loaded with AgNPs for the treatment of diabetic foot ulcer. AgNPs were synthetized with a green synthetic approach using Olive Leaf dry extract (OLE), as a reducing agent, in a reaction with Silver Nitrate (AgNO\({}_{3}\)), to reduce Ag\({}^{+}\) in Ag\({}^{0}\). The synthesis reaction between OLE and AgNO\({}_{3}\) was performed at room temperature, under stirring conditions and for 20 min. At the end of this time, the obtained nanoparticles were purified by centrifugation at 9000 rpm for 30 min and, then, washed thrice with distilled water, frozen, and lyophilized for further use. The obtained AgNPs were tested in terms of shelf stability and antioxidant activity, to evaluate, so, their possible use for the development of wound healing dressing. Once the shelf stability and the antioxidant activity were confirmed, AgNPs were loaded in HyDrO-DiAb hydrogel. The synthetized HyDrO-DiAb was characterized in terms of Water Content (%) and Moisture Retention Capacity (%) comparing the results with a hydrogel synthetized with only Chi and CMC. The obtained data evidence that are no statistical differences between HyDrO-DiAb and Chi-CMC hydrogel. Both maintain the same swelling behavior and water retention capacity. The in vitro cytotoxicity of HyDrO-DiAb was evaluated by MTT assay on HaCaT cell lines by exposing these cell lines to different concentrations of HyDrO-DiAb for 24 h. Results revealed that the cytotoxicity of sample was dose dependent and there is not cytotoxicity effect of HyDrO-DiAb sample, in the tested concentrations, since the cells growth inhibition rate was lower than 70%. In the aim to evaluate the release profile of AgNPs from HyDrO-DiAb, the tested sample was immersed for 8 h at 37\({}^{\circ}\)C in a PBS pH 8 and, at fixed time intervals, the absorbance of supernatant was measured at 430 nm. The obtained data show that the release profile of AgNPs was very fast, moreover, after 8 h, more than 90% of nanoparticles was released from the hydrogel. Finally, the wound healing ability of HyDrO-DiAb was evaluated by the cell scratch method on HaCaT cells, testing different concentrations of hydrogel for 24 h. The obtained results evidence that low concentrations of HyDrO-DiAb can in vitro close a wound and, so, silver nanoparticles used in this formulation could affect positively the wound healing process. In summary, the performed studies evidence that the synthetized HyDrO-DiAb hydrogel may be potentially useful in wound management. These results point out the positive effect of the realized nanomaterials in speeding up the wound healing process, highlighting therefore, their potential application in wound repair. This research was supported by MIUR (PON RI 2014-2020) in the framework of project Programma Operativo Nazionale FSE-FESR Ricerca e Innovazione 2014-2020, Azione I.1 \"Dottorati innovativi a caratterizzazione industriale\"--XXIII Ciclo--A.A. 2017/2018.