## 3 Results and Discussion
### The Individual Polyphenols Detected by LC-MS  
Table 4 shows the experimental results regarding the chromatographic profile of IP separated by LC-MS. Epicatechin is found in concentrations of 0.18-0.53 mg/g, quercetin 0.05-0.37 mg/g, kaempfer0.05-0.67 mg/g, gallic acid 0.08-2.17 mg/g, protocatechuric acid 0.05-0.23 mg/g, and rosmarinic acid between 3.39-9.16 mg/g.  
Figure 6: TFC for CS/FG samples extracted by CES, UAE, and MWE methods. According to the \(t\)-test, the different lower-case letters (a–h) represent the significant differences (\(p<0.05\)) between samples obtained by the same shredding method. The different capital letters (A, B) represent the significant differences (\(p<0.05\)) between samples from the same plant obtained by different shredding methods.  
Another study reported in the hydroalcoholic extract of MP found rosmarinic acid at 0.23 mg/g, rutin at 9.9 mg/g, but epicatechin was not detectable, and in the extract of SO leaf at 4.69 mg/g, rosmarinic acid but no rutin or epicatechin were reported [48].  
The group of [PERSON] and al. [53] reported the content of individual polyphenols in different plant extracts belonging to the Lamiaceae family. They identified gallic acid in ethanolic extract of SO (0.09 mg/g), TV (0.13 mg/g), MP (0.06 mg/g), MO (0.07 mg/g), OV (0.05 mg/g), protocatechuric acid in TV (0.13 mg/g) and in MO (0.34 mg/g), but this compound was undetectable in SO, MP and OV extract. Rosmarinic acid was the most relevant individual polyphenol found at a concentration of 25.2 mg/g in SO, 45.8 mg/g in TV, 72.4 mg/g in MO, 51.8 mg/g in MP, and 17.46 mg/g in OV extract. Epicatechin was detected at a concentration between 0.41 and 1.32 mg/g in SO, MP, and OV but was undetectable in TV and MO, while the highest concentration of kaempfer was 0.37 mg/g in TV extract [53].  
[PERSON] et al. obtained in the alcoholic extract of HO a content of hydroxycinnamic acids below 0.2 \(\mu\)g/g plant material, except for ferulic acid at 36.92 \(\pm\) 1.00 \(\mu\)g/g. Rutin was found in a concentration of 21.93 \(\pm\) 0.72 \(\mu\)g/g, quercetin 1.79 \(\pm\) 0.03 \(\mu\)g/g, quercetin 4.02 \(\pm\) 0.07 \(\mu\)g/g, and luteolin 29.10 \(\pm\) 0.19 \(\mu\)g/g [47].  
In the alcoholic extract of TS, it was identified as epicatechin (9-11 \(\mu\)g/g), quercetin (0.47-0.65 \(\mu\)g/g), rutin (2.54-2.94 \(\mu\)g/g), kaempfer (0.19-0.53 \(\mu\)g/g), and hydroxycinnamic acids: caffeic acid (0.07-0.53 \(\mu\)g/g), gallic acid (3.89-4.18 \(\mu\)g/g), and ferulic acid (2.65-3.03 \(\mu\)g/g) [54].  
\begin{table}
\begin{tabular}{l c c c c c c c c c c c c c} \hline \hline
**Compound** & **Rt** & **m/z** & **MP** & **TS** & **SO1** & **AM** & **OV** & **EP** & **HO** & **SO2** & **LA** & **MO** & **HP** & **CO** \\ \hline _Gallic acid_ & 5.175 & 169 & 0.4 \(\pm\) & 0.08 \(\pm\) & 0.07 \(\pm\) & 0.56 \(\pm\) & 0.27 \(\pm\) & 0.27 \(\pm\) & 0.29 \(\pm\) & 0.37 \(\pm\) & 0.38 \(\pm\) & 1.58 \(\pm\) & 1.50 \(\pm\) & 2.17  
PCA was applied, based on a linear correlation matrix, to the mean values of the analyzed parameters to study their contribution to total data variation (Figure 8a). Three components were obtained using PCA analysis. The first two principal components accounted for 68.72% and 18.94% of the variance, respectively, for 87.66% (Figure 8b). Furthermore, the linear (Figure 8c) and quadratic (Figure 8d) functional dependences of AA concerning TPC and TFC were determined. The most important variable integrated into the first component was AA, which was negatively correlated with it. On the other hand, TPC was positively correlated with the second component, and TFC was negatively correlated with it.