## 3 Results
### Atrazine Degrading Genetic Potential of Groundwater Bacterial Isolates
\begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline
**Strain** & **Closest Bacteria Associated** & _trzN_ & _atzA_ & _atzB_ & _atzC_ & _trzD_ & _atzD_ & _atzE_ & _atzF_ \\ \hline GW38-1 & _Microbacterium fluvii_ & - & - & - & + & - & - & - & - \\ GW38-3 & _Microbacterium hatanonis_ & - & + & - & - & - & - & - \\ GW38-4 & _Microbacterium maritypicum_ & + & - & - & + & - & - & - & - \\ GW38-5 & _Labedella endolytica_ & - & - & - & - & + & - & - & - \\ GW38-6 & _Arthrobacter agilis_ & + & + & - & - & - & - & - & - \\ GW38-7 & _Nesterenkonia halotolerans_ & + & - & - & - & + & - & - & + \\ GW38-10 & _Nocardioides nathaiensis_ & + & + & - & - & - & - & - & + \\ GW38-14 & _Pseudomonas paralactis_ & + & - & + & - & - & - & + & - \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Atrazine degrading genes present in isolated bacteria. +: positive amplification signal; -: no amplification signal. \begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Isolated Strain** & **Family** & **Closest Type Strain Associated** & **E Value** & **Identities** & **Gaps** \\ \hline GW38-1 & & _Microbacterium fluvii_ YSL3-15 & 0.0 & 98.6\% & 0/830 (0\%) \\ GW38-2 & & _Microbacterium ginseng_ Gsoli 259 & 0.0 & 98.3\% & 1/526 (0\%) \\ GW38-3 & Microbacteriaceae & _Microbacterium Tatanosus_ CC-01 & 0.0 & 99.7\% & 1/665 (0\%) \\ GW38-4 & & _Microbacterium maritypicum_ DSM 12512 & 0.0 & 99.5\% & 1/826 (0\%) \\ GW38-5 & & _Labedella endolytica_ EG 6500705 & 0.0 & 99.6\% & 2/746 (0\%) \\ GW38-6 & & _Arthrobacter agilis_ DSM 20550 & 0.0 & 97.9\% & 1/741 (0\%) \\ GW38-7 & Microcococcaceae & _Nesterenkonia halotolerans_ VMT0084 & 0.0 & 99.9\% & 1/737 (0\%) \\ GW38-8 & & _Kecuria plustris_ TAGA27 & 0.0 & 99.6\% & 2/499 (0\%) \\ GW38-9 & Dietziaceae & Dietzia maris DSM 43672 & 0.0 & 100\% & 0/695 (0\%) \\ GW38-10 & Nocardiidoaceae & _Nocardioides nathaiensis_ VMT M13091 & 0.0 & 97.8\% & 0/547 (0\%) \\ GW38-11 & Intrasporangiaceae & _Ormithinircrbium kibbrerasse_ K22-20 & 0.0 & 96.6\% & 2/832 (0\%) \\ GW38-12 & Bacillaceae & _Bacillus safensis_ FO-36b & 0.0 & 100\% & 0/597 (0\%) \\ GW38-13 & Enterococcaceae & _Enterococcus durans_ JCM 8725 & 0.0 & 99.5\% & 3/605 (0\%) \\ GW38-14 & Pseudomonadaceae & _Pseudomonas paralactis_ DSM 29164 & 0.0 & 100\% & 0/758 (0\%) \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Classification and alignment hit table of isolated strains from groundwater. Figure 2: Atrazine degradation pathway. Each reaction shows the genes screened and the name of the corresponding enzyme responsible for catalyzing the reaction. presence. Strains GW38-3, GW38-6, and GW38-10 close to _Microbacterium_, _Arthrobacter_, and _Nocardioides_, also showed potential genetic activity in _atzA_. _AtzB_ and _atzE_ genes were only amplified by the _Pseudomonas_ related strain, GW38-14. Some strains of _Microbacterium_ appeared to be responsible for _atzC_ signal. Instead of _atzD_ gene existence, signals were found for its enzyme homolog _trzD_ by strains GW38-5 and GW38-7 related to _Labedella_ and _Nesterenkonia_. Finally, only strains GW38-7 and GW38-10, closest to _Nesterenkonia_ and _Nocardioides_, showed positive amplification for _atzF_ coding enzyme probes.