## 3 Results
### Histological Analysis

\begin{tabular}{c c} \hline \hline
**Group** & **Parakeratinized** & **Keratinized** & **Ortokeratinized** & **Hyperorthokeratinized** \\ \hline Female control & + & 0 & 0 & 0 \\ Female 40\% & + & ++++ & 0 & + \\ Male control & + & 0 & 0 & 0 \\ Male 40\% & 0 & ++++ & + & 0 \\ Male 5\% & 0 & ++ & 0 & + \\ Male 5\% & 0 & ++ & 0 & + \\ Male 5\% & 0 & ++ & 0 &and acanthosis, the male 40% group was low to moderate, while the female and male 5% groups were moderate to high. Regarding the inflammatory infiltrate, in the male 5% group, this was absent, and it was minimal to none in the remaining groups. Vascularization was constant in the four alcohol-administered groups, where it was moderate to minimal. Epithelial hyperplasia is observed from the basal strata and toward the superior strata, and it is also manifested in the presence of acanthosis (Figure 1b,c,e,f). The inflammatory infiltrate is minimal (Figure 1c) and null in the male 5% group (Figure 1f). Histological changes of the tongue regarding the type of surface layer are presented in Table 3. In all alcohol-administered groups, the presence of a keratinized epithelium was observed, and it was high to moderate. In the female and male 40% groups, it was similar, with a high presence; in the female and male 5% groups, it was high to moderate. On the other hand, the female 40% and 5% groups presented moderate hyperthokeratinized epithelium, compared to the male 40 and 5% groups, which did not. Histological changes in the tongue are reported in Table 4. In all alcohol-administered groups, epithelial hyperplasia and acanthosis were observed. Vascularization was constant, from moderate to minimal. It should be noted that the presence of epithelial hyperplasia was identical in the female and male 40% groups (moderate, high, and minimal, in this order), while the female and male 5% groups were similar with a high presence. Regarding acanthosis, the female and male 5% groups had similar results, with a moderate presence (Figure 2c,f). The inflammatory infiltrate remained constant the in female 40% and 5% groups along with the male 40% group, with a minimal and null presence, while in the male 5% group, it was not observed (Figure 2b,c,e,f). \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Group** & **Epithelial Hyperplasia** & **Acanthosis** & **Chronic Inflammatory Infiltrate** \\  & & & **(Monocuclear Inflammatory Cells)** & **Vascularization** \\ \hline female control & 0 & 0 & 0 \\ female 40\% & +/++/+++ & ++/++ & 0/+ & ++/+ \\ female 5\% & ++++/++ & ++/++ & +/++ & ++/+ \\ male control & 0 & 0 & 0 & 0 \\ male 40\% & ++/+ & ++/+ & +/0 & +/++ \\ male 5\% & +++ & ++/++ & 0 & ++/+ \\ \hline \hline \end{tabular} Criteria: (0) no presence, (+) minimal presence, (++) moderate presence, (++) high presence. The results are the average of the observed samples (\(n=5\)).  
\end{table} 
Table 2: Histological changes in the cheek mucosa in rats after weekend alcohol consumption for 3 months. \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Group** & **Parakeratinized** & **Keratinized** & **Otrokeratinized** & **Hyperotokeratinized** \\ \hline Female control & 0 & + & 0 & 0 \\ Female 40\% & 0 & +++ & + & ++ \\ Female 5\% & ++ & ++ & 0 & ++ \\ Male control & + & 0 & 0 & 0 \\ Male 40\% & + & ++++ & + & 0 \\ Male, 5\% & 0 & +++ & + & 0 \\ \hline \hline \end{tabular} Criteria: (0) no presence, (+) minimal presence, (++) moderate presence, (++) high presence. The results are the average of the observed samples (\(n=5\)).  
\end{table} 
Table 3: Type of surface layer on the tongue after 3 months of weekend alcohol at 5% and 40% in both females and males. In the female control group, the lingual epithelium was keratinized, while in the female 40% and 5% groups, it was hyperorthokeratinized and hyperkeratinized, exhibited as thicker on the surface (Figure 2b,c).