## 2 Results and Discussion
### Non-Target Screening of Non-Volatile Compounds in Methanol Extract  
The dried plant was extracted with methanol and analyzed by UHPLC-ESI-HRMS. Both positive and negative modes were recorded. The probable and possible structure and/or class identification of the major compounds in terms of signal intensity in positive electrospray mode were based on their elemental composition and MS/MS spectra (Table 3). Three flavonoid glycosides, one anthocyanin glycoside, and lipid derivatives were found. The most abundant compound was sphingolipid hexadecaspharginine, compound 4 with the molecular structure C\({}_{16}\)H\({}_{35}\)NO\({}_{2}\) (Figure S4). Sphingolipids as essential bioactive cell components play an important role in miscellaneous parts of plant development as well as environmental response. Inducing programmed cell death, they participated in defence against bacteria and fungi pathogens [24]. Fatty acid amide erucamide (compound 6, C\({}_{22}\)H\({}_{43}\)NO) was the second most abundant compound (Figure S6). Erucamide participates in strong plant-microbe interactions [25]. Another compound (compound 5, C\({}_{18}\)H\({}_{28}\)O\({}_{4}\)) with a role in the defence reactions has been found and belongs to the class of octadecanoids (Figure S5). One glycerophosphoserine (compound 8, C\({}_{37}\)H\({}_{24}\)NO\({}_{9}\)P) was identified as either 1-octadecyl-2-tridecanoyl-glycero-3-phosphoserine (PS (O-18:0/13:0)) or 1-hexadecyl-2-pentadecanoyl-glycero-3-phosphoserine (PS (O-16:0/15:0)) (Figure S8). Compounds 1 (C\({}_{21}\)H\({}_{20}\)O\({}_{12}\)), 2 (C\({}_{21}\)H\({}_{20}\)O\({}_{11}\)) and 3 (C\({}_{22}\)H\({}_{22}\)O\({}_{11}\)) were classified as flavonoid C-glycosides containing hexose ring. By searching the MassBank database, the selection of possible candidates was narrowed down; compound 1 was one of following isomers: isoquercitrin, hyperoside, quercetin or spiraeoside; compound 2 was one of flavone C-glycoside; compound 3 was one of flavone C-glycoside; compound 3 was one of flavone C-glycoside (Figures S1-S3). In plants, they are involved in the  
\begin{table}
\begin{tabular}{c c c c c c c c} \hline \multirow{2}{*}{**No.**} & \multirow{2}{*}{**Compound**} & \multirow{2}{*}{**RI**} & \multicolumn{6}{c}{**Average Area\% \(\pm\) SD**} \\ \cline{3-8}  & & & **HS-Fr (f1)** & **HS-Dr (f1)** & **HS-HY (f1)** & **HS-Fr (f2)** & **HS-Dr (f2)** & **HS-HY (f2)** \\ \hline
56 & Trideane & 1302 & 0.04 \(\pm\) 0.01 & 0.05 \(\pm\) 0.00 & - & 0.01 \(\pm\) 0.00 & 0.03 \(\pm\) 0.01 & - \\
57 & Thymol & 1306 & - & - & 0.02 \(\pm\) 0.00 & - & - & - \\
58 & \(p\)-Vinylguaiacol & 1318 & - & - & 0.07 \(\pm\) 0.00 & - & - & 0.06 \(\pm\) 0.00 \\
59 & Cyclosativerse & 1372 & 0.01 \(\pm\) 0.00 & 0.04 \(\pm\) 0.00 & - & - & 0.03 \(\pm\) 0.01 & - \\
60 & \(\alpha\)-Copane & 1380 & 0.02 \(\pm\) 0.01 & 0.  
defence system, especially against UV radiation [26]. Regarding anthocyanin glycoside (compound 7, C\({}_{28}\)H\({}_{33}\)O\({}_{15}\)), it was identified either as penoidin 3-rutinoside or penoidin 3-rhammoside 5-glucose (Figure S7). The anticancer activity of the above compounds has already been demonstrated in numerous studies [27; 28; 29].  
The chromatograms obtained in the negative electrospray ionization mode gave much less intense peaks and much more intense background and were therefore not analyzed.