ITZ is a highly lipophilic but poorly water-soluble weak base (pKa = 3.7), and, thus, it is ionized only at a low pH, such as in gastric secretion. The solubility of ITZ (Figure 1) is estimated at 1 ng/mL at pH 7.0 and 4 ng/mL at pH 1.0 in water [12; 13]. Given the fact that its permeability is adequate, ITZ is included among class II drugs in the Biopharmaceutical Classification System (BCS). To overcome this problem, solubility-enhancing techniques can be used to improve the bioavailability of BCS class II drugs [14]. Micro- and nanotechnology-based approaches are one of the most suitable methods to increase the solubility and dissolution rate of an active pharmaceutical ingredient (API). Nanofibers are micro- and nano-scale structures with high porosity and a high surface area-to-volume ratio, which contribute to their dissolution-improving effects [15]. Electrospinning is a simple, economical, and versatile method for fiber production, during which an electric force is used to stretch and elongate charged threads from polymer solutions or melts, forming polymer nanofibers [16]. Numerous factors influence the electrospinning process. These parameters may be grouped in three classes: electrospinning factors (electric field, needle-collector distance, flow rate, and needle diameter), solution factors (solvent, polymer concentration, viscosity, and solution conductivity), and environmental factors (humidity and temperature) [17; 18]. Therefore, a thorough understanding of these parameters is required to produce electrospun nanofibers with favorable characteristics [19]. Experimental designs, such as fractional designs and full factorial designs, can be employed to identify the process variables and/or their coupled effects that have a significant influence on ultimately aiding production process optimization [20; 21; 22]. Figure 1: Chemical structures of itraconazole (**a**) and polyvinylpyrrolidone (**b**). Itraconazole nanofibers can be prepared via electrostatic spinning using water-soluble hydroxypropyI methylcellulose as a polymer for immediate release in oral applications [22], while, for topical application, segmented polyurethane, a non-biodegradable water-insoluble polymer, is used to ensure controlled drug delivery. Three solvent-based technologies (spray-drying, electrospinning, and air-assisted electrospinning) are similarly applicable for improving the dissolution rate of ITZ from Eudragit E-based solid dispersions; however, only the electrospinning and air-assisted electrospinning methods result in a homogenous distribution of ITZ in the polymer matrix [23]. ITZ-loaded polymer nanofibers with PVPVA64 have been previously prepared on a larger scale using high-speed electrospinning, which is a pharma-compatible scaled-up technology. The dissolution results of these electrospun fibers showed significant improvement when compared to crystalline ITZ [24]. In a follow-up study, the authors demonstrated the feasibility of the scaled-up tableting of an electrospun material with a rotary press [25]. In a recent study, nanofibrous ocular inserts were developed by electrospinning. Polymeric nanofibers of ITZ were prepared using polyvinyl alcohol-cellulose acetate and polycaprolactone-polyethylene glycol 12 000 polymeric blends. The results indicated that the nanofibers could be considered suitable for prolonged delivery of ITZ [26]. ITZ is authorized on the Romanian market only as 100 mg capsules. ITZ microfibers as an intermediary pharmaceutical form may be incorporated in gelatine capsules or compressed into tablets for their finite form. Novel antifungal drug delivery systems offer the benefits of improving antifungal pharmacokinetics with targeted delivery, thus combating drug resistance [27; 28]. Solubility enhancement can mend insufficient and variable bioavailability [29]. This study aimed to fabricate, characterize, and evaluate the in vitro antifungal performance of ITZ-loaded hydrophilic polymer-based single-jet electrospun fibers.