# DMSO Improves the Ski-Slope Effect in Direct PCR
## 4 Conclusions
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Dye**} & \multirow{2}{*}{**Locus**} & \multirow{2}{*}{**DNA Amplification**} & \multicolumn{2}{c}{**Average of Relative Fluorescence Units (\%)**} & \multirow{2}{*}{**Ratio of Change**} \\ \cline{3-3} \cline{5-6}  & & & **Prep-n-Go** & & **Prep-n-Go + DMSO** \\  & & & **(\(\mathbf{t=50}\))** & & **(\(\mathbf{t=50}\))** \\ \hline \multirow{4}{*}{Blue dye (6-FAM)} & D3S1358 & 90.5–146.5 & 100.0 \(\pm\) 9.0 & 94.6 \(\pm\) 15.1 & 5.4\% \(\mathbf{\blacktriangledown}\) \\  & vWA & 151.0–215.0 & 116.1 \(\pm\) 36.2 & 124.7 \(\pm\) 32.9 & 7.4\% \(\mathbf{\blacktriangle}\) \\  & D16S539 & 221.5–273.5 & 73.5 \(\pm\) 20.4 & 104.1 \(\pm\) 29.2 & 41.7\% \(\mathbf{\blacktriangle}\) \\  & CSF1 PO & 277.0–325.0 & 42.2 \(\pm\) 15.4 & 67.5 \(\pm\) 25.6 & 59.9\% \(\mathbf{\blacktriangle}\) \\  & TPOX & 332.5–384.5 & 34.3 \(\pm\) 14.5 & 69.3 \(\pm\) 25.0 & 101.8\% \(\mathbf{\blacktriangle}\) \\ \hline \multirow{4}{*}{Green dye (VIC)} & D8S1179 & 108.5–176.5 & 100.0 \(\pm\) 8.1 & 94.5 \(\pm\) 9.0 & 5.5\% \(\mathbf{\blacktriangledown}\) \\  & D21S11 & 179.5–246.5 & 48.2 \(\pm\) 16.7 & 62.3 \(\pm\) 19.2 & 29.2\% \(\mathbf{\blacktriangle}\) \\  & D18S51 & 255.5–347.5 & 38.5 \(\pm\) 21.1 & 53.1 \(\pm\) 18.8 & 38.0\% \(\mathbf{\blacktriangle}\) \\ \hline \multirow{4}{*}{Yellow dye (NED)} & D2S441 & 75.0–113.5 & 100.0 \(\pm\) 7.6 & 96.4 \(\pm\) 12.3 & 3.6\% \(\mathbf{\blacktriangledown}\) \\  & D19S433 & 115.5–173.5 & 63.6 \(\pm\) 15.8 & 73.8 \(\pm\) 26.9 & 16.0\% \(\mathbf{\blacktriangle}\) \\  & TH101 & 174.0–219.5 & 39.5 \(\pm\) 14.9 & 56.3 \(\pm\) 22.6 & 42.6\% \(\mathbf{\blacktriangle}\) \\  & FGA & 221.0–380.0 & 71.4 \(\pm\) 17.0 & 82.2 \(\pm\) 24.8 & 15.1\% \(\mathbf{\blacktriangle}\) \\ \hline \multirow{4}{*}{Red dye (TAZ)} & D2S1045 & 83.5–126.5 & 100.0 \(\pm\) 8.1 & 98.2 \(\pm\) 12.4 & 1.8\% \(\mathbf{\blacktriangledown}\) \\  & D5S818 & 133.5–189.5 & 103.8 \(\pm\) 31.1 & 97.7 \(\pm\) 28.6 & 5.9\% \(\mathbf{\blacktriangledown}\) \\  & D13S317 & 197.0–249.0 & 101.8 \(\pm\) 27.3 & 100.5 \(\pm\) 31.1 & 1.3\% \(\mathbf{\blacktriangledown}\) \\  & D7S820 & 256.5–304.5 & 66.6 \(\pm\) 29.8 & 70.6 \(\pm\) 22.9 & 6.0\% \(\mathbf{\blacktriangle}\) \\  & SE33 & 306.0–444.0 & 42.8 \(\pm\) 26.8 & 57.7 \(\pm\) 24.4 & 34.8\% \(\mathbf{\blacktriangle}\) \\ \hline \multirow{4}{*}{Purple dye (SID)} & D10S1248 & 80.0–132.0 & 100.0 \(\pm\) 8.1 & 97.0 \(\pm\) 10.6 & 3.0\% \(\mathbf{\blacktriangledown}\) \\  & D1S1656 & 154.0–209.5 & 72.9 \(\pm\) 23.2 & 95.3 \(\pm\) 30.4 & 30.7\% \(\mathbf{\blacktriangle}\) \\ \cline{1-1}  & D12S391 & 211.0–270.5 & 47.1 \(\pm\) 17.3 & 62.5 \(\pm\) 20.5 & 32.6\% \(\mathbf{\blacktriangle}\) \\ \cline{1-1}  & D2S338 & 275.5–355.5 & 57.5 \(\pm\) 27.7 & 100.5 \(\pm\) 32.5 & 74.7\% \(\mathbf{\blacktriangle}\) \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Dimethyl sulfoxide (DMSO) improved the relative fluorescence unit (RFU) ratio of short tandem repeat (STR) genotyping in each color channel. **Informed Consent Statement:** Written informed consent was obtained from all the study participants. **Data Availability Statement:** The data presented in this study are available on request from the corresponding author. **Conflicts of Interest:** The authors declare no conflict of interest.