## 2 Material and Methods
### Outcomes  
The primary outcomes of the current study were as follows:  
* Cardiovascular condition assessment. It was determined by the measurements of the systolic and diastolic blood pressure [SBP and DBP], the basal heart rate [HR], and the 2 Min Step Test [2 MST] [13]. The SBP and DBP were measured after 5 min in the seated position using an automatic blood pressure monitor (Omron HEM-7130, Kyoto, Japan). The basal HR was determined after 5 min in the supine position [14]. The HR was recorded using a HR monitor (Polar S810, OY, Oulu, Finland) with a chest strap. The 2 MST assesses the individual's gait in place as fast as possible for 2 min while lifting the knees to a height midway between their patella and iliac crest when standing. Performance on the test is defined as the number of right-side steps of the criterion height completed in 2 min. Two trials are carried out and the best of them is registered [13]. * Neuromuscular condition assessment. It was determined by the measurements of the dominant and non-dominant hand grip strength, the Arm Curl Test (ACT) [13], and the 30-Second Chair Stand Test (30 SCST) [15]. The grip strength was measured three times with each hand and the mean value of the three trials was used for analysis. Measurements were taken with a digital and adjustable hand dynamometer (JAMAR smart, Jamar, Lafayette, United States) according to a standard protocol. The overall maximum grip strength was recorded to the nearest 1 kg [16]. The goal of the ACT is to assess the participant's upper body strength within a specified time frame. Performance on the test is defined as the number of curls that participants can express in 30 s. Two trials were conducted, and the result recorded is the best performance from either trial [13]. The primary objective of the 30 SCST is to evaluate the participant's lower body strength and endurance. Performance on the test is defined as the number of times participants successfully rise to a full standing position and sit back down within the 30 s timeframe. Only one trial was performed [15]. The secondary outcomes of the current study were as follows:
* Basic blood analysis: It included the levels of glucose, creatine, uric acid, total cholesterol, high-density lipoproteins (HDL), low-density lipoproteins (LDL), triglycerides, total proteins, albumin, and N-terminal pro-B type Natriuretic peptide pro-hormonal (NT Pro-BNP) (only for HTR since this is a heart damage marker). * Functional mobility condition assessment: It was evaluated with the Sit and Reach Test (SRT) [17], the Back Scratch Test (BST) [13], the Functional Balance Test [18], the Timed up and Go Test (TUG) [19], and the 10-Meter Walk Test (10 MWT) [20]. The SRT assesses the participant's flexibility of the lower back and hamstring muscles. The performance is defined by measuring the distance reached from the fingertips to the edge of the box, with the best distance recorded after three trials. Three trials were carried out and the longest distance reached was recorded [17]. The BST assesses shoulder and upper body flexibility. The performance is defined by measuring the distance between the fingertips, with a zero score if they touch, a negative score if they do not, and a positive score if they overlap. The test was conducted twice on the participant's preferred side, and the best of the two trials was recorded [13]. The FBT evaluates the patient's ability to maintain balance and reach forward. During the test, the patient stands near a wall, positioning the arm closest to the wall at a 90\({}^{\circ}\) angle with a fist. The initial position of the third metacarpal head is recorded, and the patient is instructed to reach forward as far as possible without stepping. The final position of the third metacarpal is then recorded, and the test result is determined by the difference between the initial and final positions, representing the patient's reach distance. Three repetitions were conducted, and the average of the last two was noted for assessment [18].