## 3 Results
### Black Seeded Simpson (BSS) Trials
#### 3.1.2 Carbon Nitrogen Ratios and Nitrogen Accumulation  
For the BSS cultivar, the N content responded curvilinearly to nitrogen fertilization in SDM and RDM across both trials in accordance with typical luxury consumption trends (Table 2). In BSS1, inoculation affected a significant decrease in SDM nitrogen density from 2.209% to 1.977% (INOC; \(-\)0.233 \(\pm\) 0.659 %N, \(p\)\(\leq\) 0.0015), but there was no difference in root tissues. C/N ratios followed a downward curvilinear trend with INOC treatment in shoot tissue higher than NON (INOC; 2.223 \(\pm\) 0.730, \(p\)\(\leq\) 0.005). Root tissue exhibited no differences. In BSS2, no significant differences emerged for C/N ratios.  
\begin{table}
\begin{tabular}{l c c c c c c c} \hline \hline  & \multicolumn{6}{c}{**Fertilizer Treatment Provided Nitrogen Concentration (ppm)**} \\ Trial and Measure & 37.5 & 60 & 82.5 & 105 & 127.5 & 150 & 172.5 \\ BSS1 & & & & & & \\ Shoot Dry Matter (g) * & LSD: 0.235 s.e.: 0.1057 df = 28 p \(\leq\) 0.0345 & & & & \\
**INOC** & 2.2843 & 2.7927 & 2.499 & 3.524 & 3.4793 & 3.416 & 3.7387 \\ NON & 2.224 & 2.645 & 2.3493 & 3.0683 & 3.241 & 3.2527 & 3.3087 \\ Difference & 0.0603 & 0.1477 & 0.1497 & 0.4557 & 0.2383 & 0.1633 & 0.43 \\ Root Dry Matter (g) ** & LSD: \(-\)0.4251 s.e.: 0.1366 df = 28 p \(\leq\) 0.0042 & & & & \\ INOC & 0.602 & 0.1067 & 0.207 & 1.3737 & 0.7867 & 0.4473 & 0.7597 \\
**NON** & 0.7243 & 0.7117 & 0.639 & 1.913 & 1.1987 & 1.4433 & 0.629 \\ Difference & \(-\)0.122 & \(-\)0.605 & \(-\)0.432 & \(-\)0.539 & \(-\)0.412 & \(-\)0.996 & 0.1307 \\ Total Dry Matter (g) & n/s & & & & & \\ INOC & 2.8863 & 2.8993 & 2.706 & 4.8977 & 4.266 & 3.8633 & 4.4983 \\ NON & 2.9483 & 3.3567 & 2.9883 & 4.9813 & 4.4397 & 4.696 & 3.9377 \\ Difference & \(-\)0.062 & \(-\)0.457 & \(-\)0.282 & \(-\)0.084 & \(-\)0.174 & \(-\)0.833 & 0.5606 \\ BSS2 & & & & & & \\ Shoot Dry Matter (g) & n/s & & & & & \\ INOC & - & 4.7867 & 5.7825 & 5.705 & 6.3525 & 6.6475 & 6.2067 \\ NON & - & 4.47 & 6 & 5.6875 & 7.0975 & 5.715 & 6.7767 \\ Difference & - & 0.3167 & \(-\)0.218 & 0.0175 & \(-\)0.745 & 0.9325 & \(-\)0.57 \\ Root Dry Matter (g) & n/s & & & & & \\ INOC & - & 1.78 & 2.0675 & 2.135 & 2.0425 & 2.2175 & 2.2333 \\ NON & - & 1.89 & 1.92 & 2.0525 & 2.285 & 1.885 & 2.13 \\ Difference & - & \(-\)0.11 & 0.1475 & 0.0825 & \(-\)0.243 & 0.3325 & 0.1033 \\ Total Dry Matter (g) & n/s & & & & & \\ INOC & - & 6.5667 & 7.85 & 7.84 & 8.395 & 8.865 & 8.44 \\ NON & - & 6.36 & 7.92 & 7.74 & 9.3825 & 7.6 & 8.9067 \\ Difference & - & 0.2067 & \(-\)0.07 & 0.1 & \(-\)0.988 & 1.265 & \(-\)0.467 \\ \hline \hline \end{tabular}
\end{table}
Table 1: Effects of inoculation and nitrogen fertilization on Black Seeded Simpson Biomass.  
\begin{table}
\begin{tabular}{l c c c c c c} \hline \hline  & \multicolumn{6}{c}{**Fertilizer Treatment Provided Nitrogen Concentration (ppm)**} \\ Trial and Measure & 37.5 & 60 & 82.5 & 105 & 127.5 & 150 & 172.5 \\ ISS1 & & & & & & \\ SDM Nitrogen \% ** & LSD: \(-\)0.2328 s.e.: 0.06591 df = 28 p \(\leq\) 0.0015 & & & & \\ INOC & 1.290 & 1.465 & 1.930 & 1.898 & 2.268 & 2.596 & 2.390 \\
**NON** & 1.337 & 1.828 & 2.326 & 2.110 & 2.276 & 2.648 & 2.940 \\ Difference & \(-\)0.047 & \(-\)0.363 & \(-\)0.396 & \(-\)0.212 & \(-\)0.008 & \(-\)0.052 & \(-\)0.550 \\ SDM C/N Ratio ** & LSD: 2.2234 s.e.: 0.7305 df = 28 p \(\leq\) 0.0050 & & & & \\
**INOC** & 30.495 & 26.496 & 20.144 &