## 3 Result and Discussion
### Physicochemical Properties
#### 3.3.3 Free Amino Acid Profile
\begin{table}
\begin{tabular}{c c c c c c} \hline
**Compound** & **m/z** & **RT (min)** & **RI** & \multicolumn{2}{c}{**Concentration**} \\  & & & & **Control** & **Thermo-Tolerant** \\ \hline Aldehyde & & & & & \\ Pentanal & 86.07 & 3.68 & 969.00 & 249.09 \(\pm\) 0.83 & 69.44 \(\pm\) 0.63 \\ Hexanal & 100.09 & 5.30 & 1073.77 & 107.97 \(\pm\) 0.74 & ND \\ Heptanal & 114.10 & 7.13 & 1177.34 & 152.60 \(\pm\) 0.37 & 26.64 \(\pm\) 0.32 \\
4-Heptenal, (Z)- & 112.09 & 8.17 & 1235.22 & 12.37 \(\pm\) 0.27 & 154.09 \(\pm\) 0.62 \\ Nonanal & 142.14 & 10.87 & 1390.06 & 57.98 \(\pm\) 0.08 & 33.29 \(\pm\) 0.34 \\ Methional & 104.03 & 11.82 & 1447.77 & 12.02 \(\pm\) 0.61 & 10.79 \(\pm\) 0.12 \\ Benzaldehyde & 106.04 & 12.92 & 1515.97 & 602.83 \(\pm\) 0.26 & 195.73 \(\pm\) 0.12 \\
2-Nonanal, (E)- & 104.12 & 13.19 & 1533.35 & 50.58 \(\pm\) 0.27 & 12.50 \(\pm\) 0.77 \\ \hline Ketone & & & & \\ Butanone & 72.06 & 2.73 & 891.00 & 170.66 \(\pm\) 0.51 & 459.19 \(\pm\) 0.40 \\
2-3-Butanedione & 86.04 & 3.65 & 967.00 & 313.62 \(\pm\) 0.20 & 844.79 \(\pm\) 0.13 \\
2-Pentanone & 86.07 & 3.66 & 968.16 & 313.62 \(\pm\) 0.63 & 845.02 \(\pm\) 0.13 \\
2,3-Pentanedione & 100.05 & 4.92 & 1051.51 & 75.49 \(\pm\) 0.12 & 8.27 \(\pm\) 0.32 \\
3-Heptanone & 114.10 & 6.55 & 1145.18 & 28.69 \(\pm\) 0.01 & 9.11 \(\pm\) 0.01 \\
2-Heptanone & 114.10 & 7.10 & 1175.84 & 126.81 \(\pm\) 0.04 & 2045.73 \(\pm\) 0.35 \\
2-Nonanone & 142.14 & 10.80 & 1385.67 & 98.23 \(\pm\) 0.51 & 1881.56 \(\pm\) 0.87 \\
3-Octen-2-one & 126.10 & 11.11 & 1404.45 & 6.09 \(\pm\) 0.12 & ND \\
1-Propanone, 1-(2-furanyl)- & 124.05 & 13.71 & 1567.32 & 59.12 \(\pm\) 0.68 & 18.65 \(\pm\) 0.76 \\
2-Undecanone & 107.17 & 14.17 & 1597.26 & 54.96 \(\pm\) 0.32 & 222.63 \(\pm\) 0.49 \\
2-Tridecanone & 198.20 & 17.20 & 1809.13 & 12.67 \(\pm\) 0.12 & 49.41 \(\pm\) 0.77 \\ \hline Alcohol & & & & \\
2-Butanol & 74.07 & 4.48 & 1024.77 & 2.34 \(\pm\) 0.15 & 16.94 \(\pm\) 0.64 \\
1-Propanol & 60.06 & 4.69 & 1037.41 & 2.48 \(\pm\) 0.01 & 61.96 \(\pm\) 0.70 \\
1-Butanol & 74.07 & 6.69 & 1153.23 & 7.27 \(\pm\) 0.34 & 33.59 \(\pm\) 0.41 \\
1-Pentanol & 88.09 & 8.45 & 1250.99 & 380.14 \(\pm\) 0.91 & 127.93 \(\pm\) 0.18 \\
2-Heptanol & 116.12 & 9.69 & 1320.80 & ND & 419.95 \(\pm\) 0.02 \\
1-Hexanol & 102.10 & 10.24 & 1353.39 & 159.70 \(\pm\) 0.73 & 181.99 \(\pm\) 0.91 \\
1-Octen-3-ol & 128.12 & 11.84 & 1448.93 & 45.45 \(\pm\) 0.82 & 19.08 \(\pm\) 0.19 \\
1-Heptanol & 116.12 & 11.94 & 1455.01 & 117.50 \(\pm\) 0.38 & 58.30 \(\pm\) 0.23 \\
1-Hexanol, 2-ethyl- & 130.14 & 12.49 & 1488.77 & 754.34 \(\pm\) 0.94 & 1945.54 \(\pm\) 0.76 \\
2-Nonanol & 144.15 & 12.97 & 1519.23 & ND & 189.36 \(\pm\) 0.83 \\
2,3-Butaneediol & 90.07 & 13.25 & 1537.43 & 24.49 \(\pm\) 0.42 & 6641.79 \(\pm\) 0.18 \\ Linalool & 154.14 & 13.37 & 1545.42 & 46.18 \(\pm\) 0.85 & 10.41 \(\pm\) 0.62 \\
1-Octanol & 130.14 & 13.56 & 1557.23 & 31.98 \(\pm\) 0.76 & 158.46 \(\pm\) 0.10 \\
2-Undecanol & 172.18 & 15.95 & 1718.99 & 21.66 \(\pm\) 0.37 & 8.74 \(\pm\) 0.99 \\
1-Decanol & 158.17 & 16.54 & 1761.46 & 6.05 \(\pm\) 0.17 & 109.51 \(\pm\) 0.57 \\ Benzyl alcohol & 108.06 & 18.01 & 1869.40 & 28.03 \(\pm\) 0.82 & 38.95 \(\pm\) 0.35 \\
1-Dodecanol & 186.20 & 19.26 & 1966.08 & 9.24 \(\pm\) 0.74 & 9.10 \(\pm\) 0.68 \\ \hline Ester & & & & & \\ Methyl butyrate & 102.07 & 3.79 & 978.00 & 37.91 \(\pm\) 0.27 & 8.19 \(\pm\) 0.97 \\ Ethyl butyrate & 116.08 & 4.60 & 1031.81 & 51.60 \(\pm\) 0.89 & 607.84 \(\pm\) 0.47 \\ Butanoic acid, 2-methylpropyl ester & 144.12 & 6.64 & 1150.09 & ND & 26.88 \(\pm\) 0.30 \\ Ethyl hexanoate & 144.12 & 8.03 & 1227.60 & 4.25 \(\pm\) 0.51 & 168.82 \(\pm\) 0.63 \\ Butanoic acid, 3-methylbutyl ester & 158.13 & 8.62 & 1260.50 & 3.29 \(\pm\) 0.58 & 50.87 \(\pm\) 0.07 \\ Ethyl octanoate & 172.15 & 11.64 & 1436.68 & 55.99 \(\pm\) 0.21 & 182.17 \(\pm\) 0.16 \\ Ethyl decanoate & 200.18 & 14.78 & 1638.23 & 23.60 \(\pm\) 0.74 & 313.61 \(\pm\) 0.11 \\ Ethyl dodecanoate & 228.21 & 17.67 & 1844.22 & 112.40 \(\pm\) 0.14 & 107.41 \(\pm\) 0.28 \\ Methyl hexadecanoate & 270.26 & 22.29 & 2217.59 & 39.96 \(\pm\) 0.47 & 30.74 \(\pm\) 0.20 \\ Ethyl hexadecanoate & 284.27 & 22.72 & 2255.91 & 42.95 \(\pm\) 0.71 & 40.25 \(\pm\) 0.79 \\ \hline \end{tabular}
\end{table}