## 1 Introduction  
Shot hole borers in the genus _Eucuallacan_ (Coleoptera: Curculionidae: Scolytinae) are Asian ambrosia beetles that comprise a cryptic species complex newly invasive in the continental United States and in other countries. Species established in the USA include the tea shot hole borer (_E. perbevis_ [PERSON]) in Florida, and the polyphagous shot hole borer (_E. fornicatus_ [PERSON]) and Kuroshio shot hole borer (_E. kurosilio_ [PERSON]) in California [1]. Typical of ambrosia beetles, females are wood borers that colonize host trees in which they cultivate symbiotic fungi as a nutritional source for larvae and adults [2]. The exotic fungi vectored by _Eucuallacan_ spp. are phytopathogenic and induce _Fusarium_ dieback, a destructive vascular disease of avocado, woody ornamentals, and numerous native American trees [3, 4]. _Eucuallacan perbevis_ in Florida (referred to as \(E\). nr. _fornicatus_ in previous published reports [4, 5] prior to the taxonomic revision by [PERSON] et al. [1]) escalated in pest status in 2016 when extensive infestations were detected throughout the commercial avocado production area of Miami-Dade County [4]. Although tea [_Camellia sinensis_ ([PERSON]] is commonly attacked in Asia [6], avocado (_Persea americana_ [PERSON]) appears to be the preferred host in Florida [4; 5], but colonization of other fruit trees and natives has been observed [7]. Avocado is an important agricultural commodity in south Florida, with a production value of $23.6 million for the 2019-2020 season [8]. The prevalence of _Fusarium_ dieback presents additional challenges to Florida growers, who are already experiencing significant losses due to laurel wilt, a lethal vascular disease of the Lauraceae, including avocado. Laurel wilt is caused by _Raffaelea lauricola_ [PERSON]. C. [PERSON], [PERSON] and [PERSON], the primary fungal symbiont of redbay ambrosia beetle, _Xyleborus glabratus_ [PERSON] [9; 10]; however, this symbiont has since transferred laterally to multiple species of ambrosia beetle in Florida, which now function as additional vectors [11; 12]. Field monitoring of _E. perbrevis_ populations was first achieved using lures containing quercivorol, a food-based attractant emitted by symbiotic fungi [13]. Lures were deployed in either Lindgren funnel traps or sticky panel traps [4; 5; 14]. Subsequent studies showed that the \(\alpha\)-copaene lure developed for redbay ambrosia beetle [15] also improved captures of _E. perbrevis_ when combined with quercivorol [5; 16; 17]. Analysis of the commercial quercivorol lure indicated that it contained four enantiomers of \(p\)-ment-2-en-1-ol as well as \(\alpha\)- and \(\beta\)-phellandrene; the percent composition consisted of two _trans_-isomers: 48.12% (1_R_AS_)-\(p\)-ment-2-en-1-ol and 39.97% (15_AR_)-\(p\)-ment-2-en-1-ol, two _cis_-isomers: 4.95% (1_R_AR_)-\(p\)-ment-2-en-1-ol and 3.98% (1_S_AS_)-\(p\)-ment-2-en-1-ol, 1.57% \(\beta\)-phellandrene, and 1.42% \(\alpha\)-phellandrene [16]. The common name 'quercivorol' refers only to (15_AR_)-\(p\)-ment-2-en-1-ol [18], which is not the predominant component in the lure, so it is not clear which enantiomer(s) is/are attractive to _E. perbrevis_. It has also not been determined which fungal symbionts emit volatiles attractive to the beetle, warranting further investigation of the chemical ecology underpinning the interaction of _E. perbrevis_ and its fungal symbionts. In this study, we (1) prepared pure laboratory cultures of six fungal symbionts isolated from _E. perbrevis_, (2) identified and quantified the chemical emissions from each symbiont using volatile collections by headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectroscopy (GC-MS), (3) documented the pattern of Super-Q collected emissions of _cis_- and _trans_-\(p\)-ment-2-en-1-ol for 90 days by gas chromatography-flame ionization detection (GC-FID), and confirmed by GC-MS, from a symbiont that emitted both isomers, and (4) conducted binary choice bioassays with female _E.