## Materials and Methods
### Neutrophil Suspension Exposure to the Combined Magnetic Field  
Neutrophil suspension is suitable for measurements only on the day of its isolation. Therefore, the variety of control and experimental conditions applied in this study required re-isolation of fresh neutrophils for each of the experiments (i.e., different mice were used on different days). The results of each measurement were normalized to control, which was included in each experiment. As a control, we used neutrophil suspension incubated similarly to the study samples but outside the magnetic field induction device (i.e., at the geomagnetic field (GMF)).  
Neutrophils were incubated at 37.0 \(\pm\) 0.2 \({}^{\circ}\)C for 40 min at concentration of 10\({}^{6}\) cells/mL in 0.25 mL aliquots in 5 mL round-bottomed polystyrene tubes (Sarstedt, Germany) further used to measure chemiluminescence. The temperature was maintained by an UH4 circulating bath (MLW, Germany).  
In the first experiment, experimental conditions for control samples were selected. Suspension of neutrophils in the first group (\"GMF (Control)\") was incubated at the GMF (outside the device) with static component of \(\sim\)44 \(\mu\)T and a level of AMF of 15-50 nT (with the frequency of technogenic background of power lines--50 Hz). The second group (\"SMF (60 \(\mu\)T) \"sham control\") was incubated in the magnetic field induction device, at the SMF with static component intensity of 60 \(\mu\)T, though with not set parameters of amplitude and frequency of alternating component of the field. The temperature was the same for both groups. The results of these measurements are presented in Table 1.  
In the second experiment, two pairs of neutrophil suspensions were investigated. The first sample in each pair was a control that was incubated in the local GMF. The second sample in each pair was incubated in the magnetic field induction device at the CMF with static component of 60 \(\mu\)T and  
\begin{table}
\begin{tabular}{c c c} \hline
**Experimental Neutrophil Group** & **Chemiluminescenceence Intensity (\%)** & **Chemiluminescence Intensity (V)** \\ \hline GMF (Control) & 100.0 \(\pm\) 9.7 & 3.38 \(\pm\) 0.33 \\ SMF (60 \(\mu\)T) & 103.6 \(\pm\) 12.1 & 3.50 \(\pm\) 0.42 \\ \hline \end{tabular} All measurements were performed in six replicates. Based on the results of intergroup statistical comparison, \(p\) = 0.6304. The conditions of sample preparation are described in detail in the section ”Materials and Methods: CMF water treatment with a series of dilutions and vigorous shaking”. Data is presented as M \(\pm\) SD.  
\end{table}
Table 1: Comparison of intensity of chemiluminescence of neutrophil suspension after incubation under geomagnetic field conditions (“GMF (Control)”), and inside the device, static magnetic field (“SMF (60 \(\mu\)T)”), with static component of 60 \(\mu\)T, but without alternating component of CMF).  
alternating component of 100 nT. However, the alternating component of magnetic field for the second sample in the first pair was changed according to the sinusoidal regime with 12.6 Hz (designated as \"12.6 Hz, 100 nT\"), and for the second pair of suspensions (designated as \"48.5 Hz, 100 nT\")--with frequency of 48.5 Hz. Results of this experiment are presented in Table 2.  
In both experiments, the samples were exposed at the particular magnetic field settings for 40 min. The experiments were repeated 5-6 times.