## 3 Results
### Measurement of Cytokines Expression in Serum  
Immune cells produce cytokines, which are important for both innate and adaptive immunity; in addition, they are important for various immune cell metabolic processes, such as the maturation processes [33; 34; 35; 36]. In this study, we evaluated the effect of FRB administration on serum cytokine levels in mice administered with CY (Table 7). Results of evaluating the level of interleukin (IL)-1\(\alpha\) expression: CY control resulted in significantly lower expression compared to the normal control (Table 7). Administration of FRB (_p_ < 0.05) significantly increased the IL-1\(\alpha\) expression compared to the CY control group. Similar observations occurred with IL-6, where the normal control group was 157.1 \(\pm\) 18.8, and the CY control resulted in reduced IL-2 levels (96.4 \(\pm\) 11.5 pg/mL). The expression of IL-6 was significantly increased in the group treated with FRB (169.1 \(\pm\) 13.5, \(p\) < 0.001) and RB (144.6 \(\pm\) 8.8, \(p\) < 0.001) compared to the CY control group (96.4 \(\pm\) 11.5 pg/mL). In contrast to the significant increase in IL-6, the expression levels of IL-2 and IL-4 tended \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**(\(\upmu\)g/mL)** & **NC** & **CY** & **CY + GS** & **CY + FRB** & **CY + RB** \\ \hline IgM & 5.34 \(\pm\) 0.52 b,** & 3.90 \(\pm\) 0.38 c & 4.97 \(\pm\) 0.73 bc & 5.78 \(\pm\) 0.89 ab,* & 4.57 \(\pm\) 0.23 bc,** \\ IgG & 18.58 \(\pm\) 3.52 bc & 14.18 \(\pm\) 1.45 d & 17.93 \(\pm\) 1.22 c,** & 21.98 \(\pm\) 2.17 a,** & 18.48 \(\pm\) 1.52 bc,** \\ \hline \hline \end{tabular}  
*–d Different letters indicate statistically significant differences between groups using one-way ANOVA followed by [PERSON]’s test of \(p\) < 0.05. Statistical significances from the CY control group were determined by Student’s _t_-test (”_p_ < 0.05 and **_p_ < 0.01). (NC: normal control, CY: negative control, CY + GS: CY with Ginsen extract 0.5 g/kg-bw, CY + FRB: CY with RB 0.5 g/kg-bw, CY + RB: CY with RB 0.5 g/kg-bw).  
\end{table} 
Table 6: Effect of FRB administration on the plasma IgG, IgM level in BALB/c mice immunosuppressed by cyclophosphamide. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & **NC** & **CY** & **CY + GS** & **CY + FRB** & **CY + RB** \\ \hline \({}^{1}\) Initial Body & 21.08 \(\pm\) 1.10 & 21.06 \(\pm\) 1.07 & 21.04 \(\pm\) 0.90 & 21.06 \(\pm\) 0.48 & 21.00 \(\pm\) 0.83 \\ Weight & 23.90 \(\pm\) 1.00 a & 20.37 \(\pm\) 0.67 b & 23.88 \(\pm\) 1.66 a,** & 23.00 \(\pm\) 0.37 ab,** & 22.83 \(\pm\) 1.43 b,* \\ \hline \({}^{2}\) Liver & 39.73 \(\pm\) 0.32 bc & 38.55 \(\pm\) 0.95 c & 39.82 \(\pm\) 2.10 bc & 42.79 \(\pm\) 0.71 a,** & 40.08 \(\pm\) 1.81 bc \\ Thymus & 1.77 \(\pm\) 0.18 a & 1.05 \(\pm\) 0.36 b & 1.59 \(\pm\) 0.29 ab & 1.73 \(\pm\) 0.40 a & 1.45 \(\pm\) 0.43 ab \\ Spleen & 3.24 \(\pm\) 0.59 & 2.69 \(\pm\) 0.15 & 2.68 \(\pm\) 0.27 & 2.87 \(\pm\) 0.35 & 2.73 \(\pm\) 0.33 \\ Kidney & 15.45 \(\pm\) 0.15 & 15.82 \(\pm\) 0.41 & 15.57 \(\pm\) 0.45 & 15.63 \(\pm\) 1.04 & 15.28 \(\pm\) 0.56 \\ \hline \hline \end{tabular}  
*–d Different letters indicate statistically significant differences between groups using one-way ANOVA followed by [PERSON]’s test of \(p\) < 0.05. Statistical significances from the CY control group were determined by Student’s _t_-test (”_p_ < 0.05 and **_p_ < 0.01). (NC: normal control, CY: negative control, CY + GS: CY with Ginsen extract 0.5 g/kg-bw, CY + FRB: CY with RB 0.5 g/kg-bw, CY + RB: CY with RB 0.5 g/kg-bw).  
\end{table} 
Table 5: Changes in Body and organ weight in BALB/c mice. to slightly increase in the FRB (\(p\) < 0.05) and RB (\(p\) < 0.001) groups, respectively (Table 7). In the case of IL-12 (p70) expression levels, the expression level was 259.2 \(\pm\) 13.7 in the normal control group and significantly lowered in the CY control (177.5 \(\pm\) 9.0) group.