## 3 Results  
### Molecular Identification  
The results of the species identification obtained by conventional biochemical methods were compared to the results of a multiplex PCR assay. Seven discrepancies were reported. Five strains identified as _E. faccium_ by the biochemical tests were identified as _E. faecalis_ by the molecular genetic method, and two strains morphologically identified as _E. faecalis_ were confirmed as _E. faccium_ using the multiplex PCR. Due to the previously reported \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Target Gene** & **Marker Name** & **Primer Sequences F/R** & **Product Size** & **Annealing** & **References** \\  & & & **(bp)** & **Temperature** & **References** \\ \hline _sodA_ of _E. faecalis_ & FL1 & ACTTATGACTTAAC & & & & \\  & FL2 & TAATGGTGAATCTIGGTTTG & & & & \\ \hline _sodA_ of _E. faccium_ & FM1 & GAAAAACAATAGAAGAATT & & & & \\  & FM2 & TGCTTTTTTGAATTCTTTTTTA & & & & \\ \hline _TEM_ & TEM1 & AGGAAAGATGATGATGATG & & & & \\  & TEM2 & CTCGTTGGTTGGATGGC & & & & \\ \hline _aac(6\({}^{\prime}\))/apth(2\({}^{\prime\prime}\))_ & aac(6\({}^{\prime}\))/aph(2\({}^{\prime\prime}\))2 & CCAAGAGCAATAAGGGCATA & & & & \\  & aac(6\({}^{\prime}\))/aph(2\({}^{\prime\prime}\))2 & CACTATCAATACCACTACG & & & & \\ \hline _vanA_ & vanA1 & GGAAAACGACAATTGC & & & & \\  & vanA2 & GTACAATGCGGCCGTTA & & & & \\ \hline _vanB_ & vanB1 & CATCGCGCGTCCCCGAATTCAA & & & & \\  & vanB2 & GATGCGCGAAGATACCTGGCT & & & & \\ \hline _emeA_ & emeA1 & GTGACAGCCTTGTGCAGAT & & & & \\  & emeA2 & TAGTCGCTTGTTGTTG & & & & \\ \hline _as1_ & ASA 11 & GCACGCCTATTACGAACTATG & & & & \\  & ASA 12 & TAAGAAGAACATCACCACA & & & & \\ \hline _esp_ & ESP 14F & AGATTTCATCTTTG & & & & \\  & ESP 12R & AATTGATTCTTAGCATCTG & & & & \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Oligonucleotides used as amplification primers for the species-specific identification of _Enterococcus_ spp. antimicrobial resistance and quorum-sensing regulation genes. higher specificity of the PCR assay, the results obtained with this method were used for subsequent analysis [18]. According to the results performed by molecular method, 99 clinical isolates (90%) were identified as _E. faecalis_ and 11 isolates (10%) as _E. ficetium_. Most of the isolates were from urine (37 _E. faecalis_ and 5 _E. ficetium_) and wound secretions (36 _E. faecalis_ and 2 _E. ficetium_) (Table 2). Anticribbial Resistance and Prevalence of Antimicrobial Resistance Genes among Enterococcus spp. Isolates  
The antimicrobial resistance of tested isolates is presented in Table 3. The majority of the enterococci were resistant to \(\beta\)-lactams (59% E. _ficealis_, 100% E. _ficetium_), quinolones (51% E. _ficealis_, 100% E. _ficetium_), and aminoglycos (54% E. _ficealis_, 91% E. _ficetium_). Regarding the genes of antimicrobial resistance, high-level aminoglycoside (_aac(6')/ aph(2'')_)- and quinolone-resistance (_emeA_) genes were absent in only two (single isolates from urine and haemoculture) of all of the 110 enterococcal isolates tested. The presence of the _TEM_ gene was detected in six isolates (5%), even though four of them were susceptible to ampicillin, based on the disc diffusion method. The _VanA_ gene was detected in 19 (17%) _Enterococcus_ spp. but only three strains of _E. ficetium_ were resistant to vancomycin on the basis of the disc diffusion method, and a single one was both vancomycin- and teicoplanin-resistant. None of the studied isolates expressed the _VanB_ genotype. The prevalence of antimicrobial resistance genes among MDR _Enterococcus_ spp. clinical isolates is shown in Table 4. The highest rate was found for the _aac(6')/aph(2'') + emeA_ pattern (78%, \(n\) = 86).