## 1 Introduction
This route was considered as a more simple and easier procedure, being able to scale up more than other aforementioned green methods [17]. Although there were many studies that reported the plant extract-mediated nanoparticles, the diversified plant species were still not exploited completely. Depending on the plant extract types, the size, morphology and stabilizing ability of nanoparticles were different, which might affect the activity of formed nanoparticles. Thus the studies related to specific plants or plant parts using for nanoparticle formation still need to be investigated. _Achyranthes aspera_ Linn. (_A. aspera_) belongs to the Amaranthaceae family and is well-known as a traditional cure in fold medicine. It had many medicinal functions such as anticancer, antimicrobial, diuretic, antiobesity, antineoplastic, antiherpes, hepatotoxicityability, antineoplastic and immunomodulatory activities, antioxidant and neuroprotective property [18]. Besides, the essential oils of _A. aspera_ were demonstrated about an effective protection from two mosquito species including _C. quinquefusciatus_ and _Z. officinalis_[19]. Aqueous extract of aerial parts containing oleanolic acid could stabilize mast cells contributing helpfully in asthma treatment [20]. _A. aspera_ leaves were proved to enhance the wound healing rate on rat models [21]. In phytochemistry research, _A. aspera_ was reportedto contain alkaloids, flavonoids, betaine derivatives, proteins, amino acids, oligosaccharidesacharides, sugars, saponins, steroids and terpenoids [22]. So, there were a lot of studies using _A. aspera_ with various aspects, but the use of its leaf broth to synthesize nanoparticles was only in a few studies. With an attempt to explore the green silver nanoparticles from various plant species, we also utilized other plants of _Scoparia dulcis (S. dulcis)_ to make the comparison. _S. dulcis_ belongs to the Scrophulariaceae family. In folk medicine, it was well-known as a perennial herb with abundant bioactive compounds. Its medicinal activities exhibited on treatment of cancer, diarrhea, diabetes, stomachache, hepatosis, bronchitis, fever, hypertension and ulcers [23]. The phytoconstituents of _S. dulcis_ were abundantly found, those were diterpenoid, triterpenoid, alkaloids, flavonoids and steroids [23; 24]. Using the plant extracts to biosynthesize nanoparticles, many studies about _Cannabis sativa, Azadirachta indica, Jatropha curcas_, etc. were conducted [25; 26; 27; 28]. So far, there were no reports on _A. aspera_ or _S. dulcis_ leaf extract-mediated silver nanoparticles on antifungal activity against _Aspergillus niger (A. niger), Fusarium oxysporum (F. oxysporum)_ and _Aspergillus flavus (A. flavus)_. _A. niger_ belongs to the genus _Aspergillus_, and can cause black mold disease on certain vegetables and fruits. It is commonly found on dead leaves, compost piles, stored grain and other decaying vegetation [29]. _A. flavus_ is of the same genus of _A. niger_, well-known for colonizing on grains, nuts and legumes during postharvest stages. More dangerously, _A. flavus_ could cause human infection [30]. _F. oxysporum_ can destroy the plants gradually by clearing veins, yellowing leaves, stunting trees, etc. [31]. Thus, fungicides are necessary for crops to protect agriculture from being destroyed, more importantly to prevent the fungi infecting humans. However, antifungal resistance occurred, which induced the development of an alternative fungicide. Herein, two kinds of biosynthesized silver nanoparticles would be a good candidate. AgNPs would be fabricated using _A. aspera_ or _S. dulcis_ leaf extracts. The ultraviolet visible spectrophotometer (UV-vis) was carried out to realize the nanoparticle formation. The Fourier transform infrared spectrometer (FTIR) and energy dispersive X-ray analysis (EDXA) were used to show the functional groups and the elements of these AgNPs.