## 3 Results
### Fungistatic Activity--MIC and MFC Values  
The fungistatic activity of _G. hederacea_ extracts obtained via SC-CO\({}_{2}\) against five filamentous fungi, expressed as MIC and MFC values, are presented in Table 4 and Figure S1a-e in the Supplementary Materials. Based on the results, it can be stated that the tested extracts exhibited fungistatic activity towards the tested fungi; however, this activity was dependent mainly on the fungal species, whereas the extraction conditions generally did not affect the activity of the extracts. An exception was the extract obtained at 40 \({}^{\circ}\)C, which demonstrated activity against _B. cinerea_ (MIC and MFC values were 5 mg/mL), \begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{2}{*}{**Microorganism**} & **MIC/ MBC/ MBC** & \multicolumn{3}{c}{_Glechoma hederaca_ Extracts, Extraction Conditions: } \\  & **(mg/mL)** & **40 \({}^{\circ}\)C** & **50 \({}^{\circ}\)C** & **60 \({}^{\circ}\)C** \\ \hline \multicolumn{5}{c}{Gram-positive bacteria} \\ \hline _S. aureus_ ATCC 33862 & MIC & 0.3 & 2.5 & 2.5 \\  & MBC & 0.6 & \(>\)5.0 & 2.5 \\ _B. subtilis_ ATCC 11774 & MIC & 0.6 & 5.0 & \(>\)5.0 \\  & MBC & 1.25 & \(>\)5.0 & \(>\)5.0 \\ _E. faecalis_ ATCC 19433 & MIC & 0.6 & 2.5 & 2.5 \\  & MBC & 1.25 & 5.0 & 2.5 \\  & MIC & 1.25 & 1.25 & 1.25 \\ _Microoccus luteus_ ATCC 4698 & MBC & 5.0 & \(>\)5.0 & \(>\)5.0 \\ \hline \multicolumn{5}{c}{Gram-negative bacteria} \\ \hline _P. aeruginosa_ ATCC 9027 & MIC & 1.25 & 1.25 & 1.25 \\  & MBC & 2.5 & 5.0 & 2.5 \\  & MIC & 1.25 & 2.5 & 2.5 \\  & MBC & 2.5 & 5.0 & 2.5 \\  & MIC & 2.5 & 2.5 & 2.5 \\ _E. coli_ ATCC 8739 & MBC & 5.0 & 5.0 & 2.5 \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Antibacterial activity of _G. hederaca_, displayed by SC-CO\({}_{2}\) extracts, against tested indicator microorganisms. whereas extracts obtained at 50 \({}^{\circ}\)C and 60 \({}^{\circ}\)C showed no inhibition of this species in the tested concentration range. Similar results can be observed in the case of _C. albicans_, where the extract obtained at 40 \({}^{\circ}\)C demonstrated the highest antagonistic activity compared to the other tested extracts. The strongest activity of all extracts was observed towards _S. scleroorum_ with MIC and MFC values of 1.25 mg/mL, as well as towards _C. albicans_ (MIC and MFC at the level of 1.25 mg/mL), but only for the extract obtained at 40 \({}^{\circ}\)C. Among the _Fusarium_ strains, higher sensitivity to the tested _G. hederaca_ extracts was observed for _F. graminearum_ (MIC and MFC at 2.5 mg/mL) compared to _F. culmorum_ (MIC and MFC at 5.0 mg/mL level). No fungistatic activity of the tested extracts was observed against _A. alternata._  
Interesting data about the diversified fungistatic activity of different plant extracts obtained via SC-CO\({}_{2}\) can be found in the literature. Many authors reported that the antagonistic activity of the extracts strongly depends on the fungal genus, which was also underlined in this study. [PERSON] et al. (2019) [PERSON] et al. (2019) showed no differences in the fungistatic activity of extracts obtained via SC-CO\({}_{2}\) from _Lupinus albscens_ against _F. oxysporum_ and _F. verticilloides_, which is similar to the result obtained in the present work (the same MIC and MFC values were obtained for _F. graminearum_ and _F. culmorum_). The high dependence of the antifungal activity of extracts of guayusa leaves (_Ilex guayusa_ Loes.) obtained by SC-CO\({}_{2}\) on the type of indicator microorganism was shown by [PERSON] et al. (2019) [PERSON] et al. (2019). The studies presented in the mentioned paper showed little impact (in the case of _E. flocosum_, _M. canis_, _M. gypseum_ and _T. mentagrohydtes_) or no influence (in the case of _A. fumigatus_, _Rhizopus_ and _C. albicans_) of the extraction conditions tested on the fungistatic activity of the extracts obtained via SC-CO\({}_{2}\) from guayusa leaves [PERSON] et al. (2019). In the presented study, a minor influence of extraction conditions on the fungistatic activity of _G.