## 3 Results and Discussion
Basic Yellow 28 showed a precursor ion at \(m/z\) 322.34 and product ions at \(m/z\) 160.24 and 136.18. Basic Blue 26 showed a precursor ion at \(m/z\) 470.42 and product ions at \(m/z\) 349.30, 454.38, and 333.28. Based on the above, unknown peaks 1, 2, 3 were tentatively identified as Basic Red 2, Basic Yellow 28, and Basic Blue 26, respectively. Hence, the developed method was found to be suitable for the detection and quantitation of illegal synthetic colorants added to cosmetics. In the case of Basic Red 2, several peaks ascribed to impurities were observed and marked as such in the chromatograms of Figure 3 [32]. The MS/MS fragmentation patterns of the three detected compounds (Basic Blue 26, Basic Red 2, and Basic Yellow 28) were examined via LC-Q-TOF-MS at different collision energies. The optimized MS/MS spectra and the related ion structures are presented in Figures 4-6. Basic Blue 26 exhibited a precursor ion at \(m/z\) 470.26 (C\({}_{33}\)H\({}_{32}\)N\({}_{3}\)\({}^{+}\)), and the difference from the theoretical value was estimated to be within 0.64 ppm. Fragment ions were observed at \(m/z\) 454.23, 333.14, and 349.17. Basic Red 2 showed a precursor ion at \(m/z\) 315.16 (C\({}_{20}\)H\({}_{19}\)N\({}_{4}\)\({}^{+}\)), and the difference from the theoretical value was estimated to be within 1.90 ppm. Fragment ions at \(m/z\) 237.11, 210.10, and 299.13 were observed. Basic Yellow 28 showed a precursor ion at \(m/z\) 322.19 (C\({}_{20}\)H\({}_{24}\)N\({}_{3}\)O\({}^{+}\)), and the difference from the theoretical value was estimated to be within 1.24 ppm. Fragment ions were observed at \(m/z\) 160.11 and 136.08. Figures S1-S10 present the fragmentation patterns of 10 colorants other than those detected in cosmetics samples. Disperse Brown 1 exhibited a precursor ion at \(m/z\) 433.02 (C\({}_{16}\)H\({}_{16}\)Cl\({}_{3}\)N\({}_{4}\)O\({}_{4}\)\({}^{+}\)) and fragment ions at \(m/z\) 356.97, 197.05, and 153.02. Disperse Orange 3 presented a precursor ion at \(m/z\) 243.09 (C\({}_{12}\)H\({}_{11}\)N\({}_{4}\)O\({}_{2}\)\({}^{+}\)) and fragment ions at \(m/z\) 122.02 and 197.09. HC Blue No. 2 showed a precursor ion at \(m/z\) 286.14 (C\({}_{12}\)H\({}_{20}\)N\({}_{3}\)O\({}_{5}\)\({}^{+}\)) and fragment ions at \(m/z\) 227.13 (C\({}_{13}\)H\({}_{15}\)N\({}_{4}\)\({}^{+}\)) and fragment ions at \(m/z\) 77.04 and 134.07. Solvent Yellow 1 showed a precursor ion at \(m/z\) 198.10 (C\({}_{12}\)H\({}_{12}\)N\({}_{3}\)\({}^{+}\)) and fragment ions at \(m/z\) 77.04, 92.05, and 105.04. Solvent Yellow 3 exhibited a precursor ion at \(m/z\) 226.13 (C\({}_{14}\)H\({}_{16}\)N\({}_{3}\)\({}^{+}\)) and fragment ions at \(m/z\) 291.05, 106.07, and 121.08. Disperse Yellow 3 presented a precursor ion at \(m/z\) 268.11 (C\({}_{15}\)H\({}_{14}\)N\({}_{3}\)O\({}_{2}\)\({}^{-}\)) and fragment ions at \(m/z\) 134.06 and 92.05. HC Yellow No. 5 exhibited a precursor ion at \(m/z\) 196.07 (C\({}_{8}\)H\({}_{10}\)N\({}_{3}\)O\({}_{3}\)\({}^{-}\)) and fragment ions at \(m/z\) 166.06 and 137.04. To the best of our knowledge, the fragmentation patterns of the 11 out of the 13 examined dyes have not been reported before, i.e., only the fragmentations of \begin{table}
\begin{tabular}{c c c c c c} \hline
**Compound** & **Chemical Formula** & **Ion Mode** & **Precursor Ion (\(m/z\))** & **CV (V)** & **Product Ion (\(m/z\))** & **CE (V)** \\ \hline Solvent Red 24 & C\({}_{24}\)H\({}_{21}\)N\({}_{4}\)O & + & 381.35 & 15 & 106.17 \({}^{*}\) & 30 \\  & & & & 224.23 & 20 \\ \hline Solvent Yellow 1 & C\({}_{12}\)H\({}_{11}\)N\({}_{3}\) & + & 198.30 & 15 & 77.24 \({}^{*}\) & 20 \\  & & & & 93.25 & 20 \\ \hline Solvent Yellow 3 & C\({}_{14}\)H\({}_{15}\)N\({}_{3}\) & + & 226.33 & 15 & 106.24 \({}^{*}\) & 20 \\  & & & & 121.24 & 20 \\ \hline \end{tabular} \({}^{*}\) Quantitative ion; MRM, multiple reaction monitoring; CV, cone voltage; CE, collision energy.  
\end{table} 
Table 5: _Cont._Solvent Orange 7 and Solvent Red 24 have been described [33].