## 3 Results and Discussion
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multirow{2}{*}{**Sample**} & **Ticlosan** & \multicolumn{3}{c}{**Dry Condition**} & \multicolumn{3}{c}{**Humidity Condition**} \\ \cline{2-7}  & **(ppm \({}^{\star}\))** & **Inhibition** & \multicolumn{2}{c}{**Microscopy (Cells/cm\({}^{2}\))**} & **Inhibition** & \multicolumn{2}{c}{**Microscopy (Cells/cm\({}^{2}\))**} \\ \cline{2-7}  & **Size (cm)** & **Live** & **Dead/Injured** & **Size (cm)** & **Live** & **Dead/Injured** \\ \hline Id1 & 0 & - & \(1.63\times 10^{5}\) & \(2.58\times 10^{4}\) & - & \(1.47\times 10^{6}\) & \(2.83\times 10^{3}\) \\ Id2 & 400 & \(0.36\pm 0.05\) \({}^{\rm c,B}\) & ND & \(3.11\times 10^{1}\) \({}^{\rm b}\) & \(0.48\pm 0.07\) \({}^{\rm c,A}\) & \(0.58\times 10^{1}\) \({}^{\rm b}\) & \(1.95\times 10^{1}\) \({}^{\rm b}\) \\ Id3 & 500 & \(0.61\pm 0.08\) \({}^{\rm b,A}\) & ND & \(2.08\times 10^{2}\) \({}^{\rm b}\) & \(0.56\pm 0.05\) \({}^{\rm b,A}\) & \(2.56\times 10^{1}\) \({}^{\rm b}\) & \(0.11\times 10^{1}\) \({}^{\rm b}\) \\ Id4 & 650 & \(0.63\pm 0.07\) \({}^{\rm b,A}\) & ND & \(1.76\times 10^{2}\) \({}^{\rm b}\) & \(0.64\pm 0.07\) \({}^{\rm b,A}\) & ND & ND \\ Id5 & 850 & \(0.88\pm 0.05\) \({}^{\rm a,A}\) & ND & \(8.42\times 10^{1}\) \({}^{\rm b}\) & \(0.95\pm 0.06\) \({}^{\rm a,A}\) & ND & ND \\ \hline \hline \end{tabular}  
* Values (means \(\pm\) s.d.) in the same column with different letters are significantly different (\(p<0.05\)). \({}^{\rm a,B}\) Values (means \(\pm\) s.d.) in the same file (capital letters) with different letters are significantly different (\(p<0.05\)). (\({}^{\rm b}\)) Parts per million concentrations. ND: Not detected by epifluorescence microscopy.  
\end{table} 
Table 2: Bacterial inhibition (size of the inhibition is expressed in cm) of _S. aureus_ on surfaces treated with triclosan (400 to 850 ppm) or not treated (0 ppm) after 24 h incubation. The presence of live and dead or injured cells by epifluorescence microscopy are expressed in cells/cm\({}^{2}\). Figure 2: Correlation (R\({}^{2}\)) between triclosan concentration (ppm) and the bacterial inhibition (cm) under dry and humid conditions, tested on the polyester surfaces. N = 9 for each result. adequate conditions for biofilm formation were not present (Table 2). Additionally, the drying of the surfaces should not be disregarded during the sanitation process because the spread of bacteria in a liquid medium is essential for their survival [56]. Triclosan is incorporated in different materials to provide protection against bacterial growth. However, there is concern about the increased use of triclosan and its possible adverse effects on human health [23; 24; 57; 58]. These effects include endocrine disruption, cytotoxicity, carcinogenic and neurotoxic effects, and reproductive disorders [59; 60]. In addition, studies monitored in humans have detected triclosan in urine, blood and breast milk [21]. However, these adverse results on human health after exposure to triclosan are not yet clear [61]. [PERSON] et al. [62] observed that the health problems attributed to triclosan are not necessarily being produced exclusively by this agent and can also be attributed to other chemical products with similar properties. In real usage, triclosan is not expected to cause health effects when products containing this agent are used according to indications [21]. However, a prudent use of triclosan is recommended [63]. The legislation concerning triclosan has changed over the years in Europe; however, in the US, legislation on the use of this antimicrobial agent is unclear. In Europe, according to a European Decision (2010), triclosan was banned as an additive and its use in any materials in contact with food [20]. In the USA, although the FDA banned the use of triclosan in hand soaps in 2016 [64], this substance continues to be used in other types of products. According to the legislation of this country, the regulation on the use of triclosan has not presented much progress since the first draft in 1974, which was then updated in 1994.