## 3 Results  
### Influence of the Conventional Extraction Parameters on the TPC, TFC, and TAA of the Extracts  
The knowledge of the extraction conditions and their influences is essential in order to obtain the highest concentration of biologically active compounds from the raw material. In our study, the three-factor, three-level Box-Behnken design (BBD) with three responses in the central point was applied in order to study the effects of different extraction parameters on the TPC, the TFC, and the TAA of the comelian cherry extracts. The BBD experimental design for the conventional extraction and the experimental results are presented in Table 1.  
The antioxidant capacity of the comelian cherry depends on the cultivar and cultivation conditions (geographical region, climatic parameters, maturity, and collection period) [27]. In our study, the highest TAA value (29.83 \(\pm\) 0.85 mg TE/g dw) was obtained in conventional extraction with hydroalcoholic solution at an ethanol concentration of 60% (\(v/v\)) after 15 min of extraction at 40 \({}^{\circ}\)C. In the same extraction conditions, the highest concentration of TPC (29.27 \(\pm\) 1.09 mg GAE/g dw) was found. Increasing the extraction time up to 30 min, at the temperature of 30 \({}^{\circ}\)C, allowed us to obtain the highest concentration of TFC (1.61 \(\pm\) 0.01 mg QE/g dw), but a lower value of TPC (27.62 \(\pm\) 0.36 mg GAE/g dw), approximatively 6% lower than the value obtained after 15 min of extraction at 40 \({}^{\circ}\)C. When the temperature was increased up to 50 \({}^{\circ}\)C in the same extraction time (30 min) and at the same solvent concentration (60% EtOH), 26.68 \(\pm\) 0.52 mg GAE/g dw was found, a value 10% lower than the result obtained at 40 \({}^{\circ}\)C and 15 min. Our results indicate that the concentration of the hydroalcoholic solution was the main independent variable influencing the extraction, and the higher concentrations of bioactive compound recovery from the comelian cherry extracts were obtained at 60% (\(v/v\)). These results are in good agreement with [PERSON] et al. [15] who reported the highest valuesfor TPC at temperatures up to 35 \({}^{\circ}\)C and an ethanol concentration between 50-70%. The analysis of the influence of the independent variables on the TAA (mg TE/g dw) and on the TPC concentration (mg GAE/g dw) allowed us to obtain a good correlation between both response parameters (Figure 1). As expected, Figure 1a shows an increase in the antioxidant activity with the TPC content of the extracts, as well as with the TFC content (Figure 1b). The Pearson product-moment correlation between the TAA and the TPC revealed a statistical correlation between those two variables (\(p\)-value was 0.000, at a 95% confidence level; with a correlation coefficient of 0.99), as well as between the TAA and the TFC (\(p\)-value was 0.000, at a 95% confidence level; with a correlation coefficient of 0.93). The statistical software allowed us to obtain the F-test ratio, the coefficient of regression R\({}^{2}\), the R\({}^{2}\) adjusted for D.F., the Durbin-Watson statistic, and the \(p\)-values of the predicted  
Figure 1: Correlation of TPC (**a**) and TFC (**b**) values with the TAA for the conventional extraction. model for the TPC, the TFC, and the TAA from the cornelian cherry conventional extracts (Table 2). The ANOVA table partitions were applied in order to observe the variability of the response variables in separate pieces for each of the effects. The test of the statistical significance for each effect was applied by comparing the mean square against an estimate of the experimental error. In Table 2, it can be observed that in the case of all three response variables (TPC, TFC and TAA) the amount of ethanol in the hydroalcoholic solution has a statistically significant effect (\(p<0.05\)), as also described by the Pareto diagram (Figure 2).