## 3 Microbiological Degradation of EDCs
Fungi from genus _Trichoderma_, _Trametes_, _Panerochaete_, _Pleurotus_, _Apergillus_, _Lentinula_, _Drechslera_, _Curvularis_, _Penicillium_, _Acernonium_, _Fusarium_, _Giberella_, _Beauvaria_, _Doaratomyes_, _Scopulariopsis_, _Chaotnidium_, _Thielavia_, _Neurospora_, _Absidia_, _Cunningham_, _Actinomucr_, _Rhizopus_, _Phycomyces_, _Morierella_, _Circinella_ and _Gandorma_ (Table 2) were also described as being capable of hormone biodegradation/biotransformation [42; 43; 44; 45; 22; 30]. The biodegradation processes of estrogens with the participation of bacteria occurs under both aerobic and anaerobic conditions, in both monosubstrate conditions and in cometabolic cultures [3; 7; 21; 33; 34; 35]. [PERSON] et al. [3] observed the biotransformation of 17\(\beta\)-estradiol to estrone under both aerobic and anaerobic conditions by bacteria isolated from deep sea sediments of mud volcanoes of the Gulf of Cadiz. The appearance of three intermediates of conjugated estrogen (17\(\alpha\)-estradiol-3-sulfate) degradation has also been observed in both aerobic and anaerobic environments. However, degradation occurs much faster in an aerobic environment. Moreover, the main degradation mechanism varied depending on the oxygen limitation. Under aerobic conditions, the reaction initiating degradation was the oxygenation of position 17 of the 17\(\alpha\)-estradiol-3-sulfate ring, which led to estrone-3-sulfate, while under anaerobic conditions the first degradation step was the deconjugation of the thioester bond at position 3, resulting in 17\(\alpha\)-estradiol. In the second stage of degradation, the appearance of estrone was observed in both aerobic and anaerobic conditions [21]. As well as the degree of oxygenation, microbial degradation of hormones depends on the type and concentration of the endogenous substance, temperature, amount of suspended particles, age of activated sludge and additional carbon sources. A reduction of estradiol degradation was observed in the presence of glucose, sodium acetate and sodium citrate, whereas in the presence of methanol \begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Organism** & **EDCs** & **Dose** & **Efficiency [\%]** & **References** \\ \hline _Pleurotus ostreatus_ & dimethyl phthalate & 100 \(\upmu\)M & 50.00 & [30] \\  & methyl paraben & 100 \(\upmu\)M & 100.00 & [30] \\  & butyl paraben & 100 \(\upmu\)M & 98.00 & [30] \\  & nonylphenol & 100 \(\upmu\)M & 68.00 & [30] \\  & bisphenol A & 100 \(\upmu\)M & 60.00 & [30] \\ _Planetochaete chrysosporium_ & dimethyl phthalate & 100 \(\upmu\)M & 65.00 & [30] \\  & methyl paraben & 100 \(\upmu\)M & 69.00 & [30] \\  & butyl paraben & 100 \(\upmu\)M & 58.00 & [30] \\  & nonylphenol & 100 \(\upmu\)M & 69.00 & [30] \\  & bisphenol A & 100 \(\upmu\)M & 66.00 & [30] \\ _A Aspergillus brassiliensis_ & progesterone & 10 mg/L & no data & [42] \\ _Absidia coordinate_ & vKM F-833 & androst-4-ene-3,17-dione & 1 g/L & 29.00 & [43] \\ _Bauveria basiana_ & vKM & androst-4-ene-3,17-dione & 1 g/L & 65.00 & [43] \\ F-2533 & androsta-1,4-diene-3,17-dione & 1 g/L & 30.00 & [43] \\ _Drechslera_ sp. Ph F-34 & androst-4-ene-3,17-dione & 1 g/L & 4.00 & [43] \\  & androsta-1,4-diene-3,17-dione & 1 g/L & 8.00 & [43] \\  & testosterone & 1 g/L & 100.00 & [43] \\ _Gibberella zue_ & vKMF-2600 & androsta-1,4-diene-3,17-dione & 1 g/L & 7.00 & [43] \\ _Cunningham echiculuta_ & androst-4-ene-3,17-dione & 1 g/L & 6.00 & \\ _Cunningham echiculuta_ & testosterone & 200 mg/L & no data & [44] \\  & 17\(\alpha\)-ethynylestradiol & 0.8 mg/L & no data & [44] \\ _Apergillus nitiulans_ & vKPM & progesterone & 1 g/L & no data & [45] \\ F-1069 & progesterone & 7 g/L & no data & [46] \\ _Circinella muscae_ & progesterone & 7 g/L & no data & [46] \\  & testosterone enanthate & 7 g/L & no data & [46] \\ \hline \hline \end{tabular}
\end{table} 
Table 2: _Cont._degradation was faster. Moreover, it was shown that the degradation rate depended on the carbon to nitrogen ratio - the lower it was, the faster the estrogen decomposition was.