## 3 Results
### GC-MS Analysis of the Chemical Composition of SJW EO
\begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Peak** & **RT** & **RI** & **Name** & **\% of TIC** \\ \hline \multicolumn{5}{c}{**Alkanes**} \\
1 & 8.68 & 900 & n-Nonane & 5.85 \(\pm\) 0.12 \\
5 & 11.03 & 966 & 3-Methyllnonane & 5.63 \(\pm\) 0.11 \\
11 & 14.01 & 1077 & 2-Methyldecane & 3.42 \(\pm\) 0.24 \\
12 & 15.11 & 1101 & n-Undecane & 1.80 \(\pm\) 0.13 \\
18 & 19.94 & 1299 & 2-Methyldodecane & 0.49 \(\pm\) 0.04 \\ \multicolumn{5}{c}{**Alkylbenzene**} \\
8 & 12.69 & 1022 & p-Cymene & 2.05 \(\pm\) 0.33 \\ \multicolumn{5}{c}{**Monoterpenes**} \\
2 & 9.46 & 923 & \(\alpha\)-Thujene & 0.90 \(\pm\) 0.09 \\
3 & 9.78 & 930 & \(\alpha\)-Pinene & 27.52 \(\pm\) 1.05 \\
4 & 10.23 & 948 & Camphene & 0.39 \(\pm\) 0.07 \\
6 & 11.19 & 977 & \(\beta\)-Pinene & 10.08 \(\pm\) 1.43 \\
7 & 11.55 & 989 & \(\beta\)-Myrvene & 0.46 \(\pm\) 0.08 \\
9 & 12.84 & 1026 & Limonene & 0.89 \(\pm\) 0.78 \\
10 & 13.40 & 1045 & \(\beta\)-Ocimene & 0.80 \(\pm\) 0.09 \\ \multicolumn{5}{c}{_Oxygenated monoterpenes_} \\
13 & 16.36 & 1136 & L-Pinocarveol & 0.41 \(\pm\) 0.13 \\
14 & 16.51 & 1142 & (E)-Verbenol & 0.46 \(\pm\) 0.10 \\
15 & 18.00 & 1187 & \(\alpha\)-Terpineol & 0.69 \(\pm\) 0.23 \\
16 & 18.33 & 1203 & Verbene & 0.52 \(\pm\) 0.11 \\
17 & 18.65 & 1238 & (E)-Chrysanthenyl acetate & 0.43 \(\pm\) 0.06 \\ \multicolumn{5}{c}{**Sesquiterpenenes**} \\
19 & 23.01 & 1376 & \(\alpha\)-Copaene & 0.60 \(\pm\) 0.06 \\
20 & 23.23 & 1390 & \(\beta\)-Elemene & 0.44 \(\pm\) 0.11 \\
21 & 24.07 & 1412 & \(\beta\)-Funebrene & 0.80 \(\pm\) 0.14 \\
22 & 24.21 & 1417 & \(\beta\)-Caryophyllene & 6.77 \(\pm\) 1.23 \\
23 & 24.31 & 1423 & \(\beta\)-Cedrene & 0.43 \(\pm\) 0.07 \\
24 & 24.42 & 1434 & \(\beta\)-Copaeene & 0.55 \(\pm\)0.05 \\
25 & 24.65 & 1439 & Aromandendrene & 0.73 \(\pm\) 0.08 \\
26 & 24.95 & 1444 & (Z)-\(\beta\)-Farrnesene & 0.47 \(\pm\) 0.10 \\
27 & 25.07 & 1452 & \(\alpha\)-Caryophyllene & 0.31 \(\pm\) 0.05 \\
28 & 25.58 & 1478 & \(\gamma\)-Muurolene & 2.76 \(\pm\) 0.75 \\
29 & 25.76 & 1486 & Germacrene D & 6.37 \(\pm\) 0.87 \\
30 & 25.98 & 1492 & Viridiforene & 0.95 \(\pm\) 0.07 \\
31 & 26.07 & 1499 & Bicyclogermacrene & 0.39 \(\pm\) 0.07 \\
32 & 26.18 & 1505 & \(\alpha\)-Muurolene & 0.89 \(\pm\) 0.23 \\
33 & 26.50 & 1516 & \(\gamma\)-Cadinene & 1.71 \(\pm\) 0.11 \\
34 & 26.62 & 1524 & \(\delta\)-Cadinene & 1.74 \(\pm\) 0.21 \\
35 & 26.69 & 1533 & (E)-Cadina-1,4-diene & 0.77 \(\pm\) 0.12 \\
36 & 26.88 & 1541 & \(\alpha\)-Cadinene & 0.61 \(\pm\) 0.14 \\ \multicolumn{5}{c}{_Oxygenated sesquiterpenenes_} \\
37 & 27.62 & 1560 & (E)-NerolidVariations in the chemotypes and chemical compositions of EOs among the SJW species growing in Bulgaria were also observed. [PERSON] et al. [28] investigated EOs obtained from the flowers and leaves of seven Bulgarian _Hypericum_ species--_H. perforatum_, _Hypericum cerastoides_, _Hypericum rumeliacum_, _Hypericum montbretii_, _Hypericum muscularum_, _Hypericum hirsutum_ and _Hypericum calycinum_. The identified EO constituents belonged to four chemical classes--alkanes, monoterpenes, sesquiterpenes and fatty acids. The main class of compounds detected in _H. muscularum_ and _H. perforatum_ were sesquiterpenes. _H. montbretii_ EO contained monoterpenes (38.09%) and sesquiterpenes (37.09%) as major groups, while _H. hirsutum_ EO contained predominately alkanes (67.19%). Interestingly, _H. hirsutum_ EO contained cedrol (5.04%), identified for the first time in _Hypericum_ species. Fatty acids were the main compounds in _H. cerastoides_, while monoterpenes were the most abundant class in _H. rumeliacum_ and _H. calycinum_ EOs. \(\alpha\)-Pinene and germacrene D were the major EO constituents of all analysed _Hypericum_ species except for _H. hirsutum_ and _H. cerastoides_. The analysis of EO from _H. perforatum_ harvested in 2020 from the region of Dulovo, Dobrich district, Bulgaria, showed that oxygenated monoterpenes were 5.4 times higher, but monoterpene hydrocarbons were 11.5 times lower in comparison with the sample we studied.