# Comparison of Three Biological Control Models of _Pycnoporus__sanguineus_ on Phytopathogenic Fungi
## 3 Results  
The PIRG values among the different treatments evaluated against _B. cinerea_ using ANOVA indicate that in the dual tests (Model 1), _P. sanguineus_ had a very slight antagonistic effect when both grew together. According to the PIRG evaluation, _B. cinerea_ was inhibited only 2.78% by _P. sanguineus_, which is the lowest inhibition percentage among the three models (\(p<0.05\)). A higher inhibition value was observed for cinnabarin in Model 2 (16.81%, \(p<0.05\)), while those of captan and benomyl within the same model, as well as the treatments of PDA with _P. sanguineus_ extract and PDA with benomyl in Model 3, showed much higher rates of inhibition (84.85%, 94.83%, 74.34%, and 87.55%, respectively, \(p<0.05\)). However, the addition of captan to PDA in Model 3 inhibited its growth completely (100%, \(p<0.05\); Table 1). The PIRG values among the different treatments evaluated against _F. oxysporum_ using ANOVA indicate that in Model 1, _P. sanguineus_ had a moderate antagonistic effect that was similar to those found for cinnabarin and benomyl in Model 2. However, they were lower than captan in Model 2 and PDA with _P. sanguineus_ extract in Model 3. The treatments of the PDA with captan and benomyl in Model 3 presented even higher inhibition values. The GR values among the different treatments evaluated against _B. cinerea_ using ANOVA indicate that _B. cinerea_ that grew alongside _P. sanguineus_ in Model 1 was not different from what was presented in the control treatments of Models 1, 2, and 3 (7.84 mm/day, 7.77 mm/day, and 7.61 mm/day, respectively, \(p<0.05\)). These results were also not significantly different from those of cinnabarin in Model 2 (6.6 mm/day, \(p<0.05\)). On the \begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Model’s**} & \multirow{2}{*}{**Treatment**} & \multicolumn{2}{c}{_Botrytis cinerea_} & \multicolumn{2}{c}{_Fusarium oxysporum_} \\ \cline{3-6}  & & **PIRG (\%)** & \(p\leq 0.05\) & **PIRG (\%)** & \(p\leq 0.05\) \\ \hline Model 1 & _Pycnorus sanguineus_ & (\(\pm 2.3\)) 2.78 & d & (\(\pm 2.2\)) 9.54 & d \\ \hline \multirow{3}{*}{Model 2} & Cinnabarin (CH\({}_{2}\)OH-COOH-NH\({}_{2}\)) & (\(\pm 3.8\)) 16.81 & c & (\(\pm 3.5\)) 17.79 & d \\  & Captan (C\({}_{6}\)N\({}_{5}\)N\({}_{3}\)NO\({}_{2}\)S) & (\(\pm 3.4\)) 84.85 & b & (\(\pm 4.6\)) 33.50 & c \\  & Benomyl (C\({}_{14}\)H\({}_{8}\)N\({}_{4}\)O\({}_{3}\)) & (\(\pm 2.4\)) 94.83 & b & (\(\pm 4.1\)) 15.98 & d \\ \hline \multirow{3}{*}{Model 3} & PDA + _Pycnorus sanguineus extract_ & (\(\pm 3.8\)) 74.34 & b & (\(\pm 5.3\)) 47.14 & c \\  & PDA + Captan (C\({}_{6}\)N\({}_{5}\)Cl\({}_{3}\)NO\({}_{2}\)S) & (\(\pm 0.2\)) 100 & a & (\(\pm 4.2\)) 70.25 & b \\  & PDA + Benomyl (C\({}_{14}\)H\({}_{18}\)N\({}_{4}\)O\({}_{3}\)) & (\(\pm 0.5\)) 87.55 & b & (\(\pm 1.9\)) 90.53 & a \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Mean values with standard error and ANOVA results for the percentage of inhibition of radial growth among the different treatments evaluated within each model. other hand, captan and benomyl in Model 2, as well as PDA with _P. sanguineus_ extract in Model 3, reduced its growth considerably (1.94 mm/day, 1.46 mm/day, and 3.85 mm/day, respectively, \(p\) < 0.05). Consistent with what was observed in the PIRG, the PDA with captan and benomyl almost completely inhibited its growth (0.02 mm/day and 0.93 mm/day, respectively, \(p\) < 0.05; Table 2). The _t_-test results indicate a higher resistance expressed in lower PIRG percentages by _F. oxysporum_ to the treatments of cinnabarin and benomyl in Model 2 (_p_ = 5.75 \(\times\) 10\({}^{-6}\) and \(p\) = 1.5 \(\times\) 10\({}^{-8}\), respectively), as well as the PDA with _P. sanguineus_ and captan in Model 3 (_p_ = 0.004 and \(p\) = 0.0001, respectively) (Figure 2).