## 3 Results and Discussion
### Amino Acid Composition in Biodegraded Residues  
Table 2 presents the amino acid composition of sugarcane residues subjected to biodegradation. In particular, aspartic acid from residues treated with the combination of _Colletotrichum_ spp. and _Rhizopus_ spp. spores was the amino acid with the highest incidence, with a mean of 14.11 mmol/100 g. On the other hand, in general, cysteine was the amino acid that occurred in the lowest proportion in all the biodegradated residues. On the other hand, the amount of amino acids was not directly related to the addition of microorganisms, since the control group of residues, which did not receive the application of phytopathogenic fungi, reported a higher content of amino acids than the T2 treatment, in which _Colletotrichum_ spp. spores were added. In addition, the amino acid indices showed significant differences in the concentrations between each treatment. The amino acid content in compost, in addition to indicating the degree of organic matter decomposition, can directly influence its agronomic quality. Amino acids such as aspartic acid, glutamic acid, and glycine act as precursors of organically bound nitrogen readily assimilable by soil microbiota and may also stimulate microbial activity and plant development. Several studies have shown that the amino acids present in compost or organic biofertilizers can improve nutrient uptake efficiency, promote chlorophyll synthesis, and enhance plant resistance to abiotic stress [44]. Therefore, their presence in the final  
\begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{2}{*}{**AA**} & \multicolumn{4}{c}{**Concentration (mmol/100 g)**} \\ \cline{2-5}  & **T1** & **T2** & **T3** & **T4** \\ \hline GLU & 10.71 \({}^{\text{d}}\) \(\pm\) 0.12 & 10.39 \({}^{\text{c}}\)\(\pm\) 0.02 & 9.04 \({}^{\text{b}}\)\(\pm\)  
compost product could contribute to improved soil fertility, enhanced soil structure, and more sustainable agricultural practices. Fermentation time is a key factor in the decomposition and stabilization of nitrogenous compounds, affecting the final amount of amino acids in a biodegraded product. However, in this experiment, all treatments were fermented for the same period due to equipment availability constraints and to maintain a controlled variable framework. This was a study limitation, as varying multiple experimental factors would have increased complexity and hindered the primary objective of evaluating the effect of fungal inoculation on sugarcane residue degradation. The disparities in the influxes suggest that additional factors influenced the trends, including the specific conditions of the biodegradation process, biological interactions between microorganisms, microbial activity, and the initial composition of the organic waste, among others. Amino acids are key determinants of compost quality. These functional molecules can stimulate positive effects on plant growth characteristics, including nutrient uptake, PSII (Photosystem II) quantum efficiency, translocation, and biomass production [45]. Recent studies have shown that amino acids are more critical molecules for crops than inorganic nitrogen [46]. [PERSON] et al. [47] examined the amino acid content of two types of compost made from cotton waste as a raw material. Samples of the substrates were evaluated at the end of the thermophilic, mesophilic, and curing stages. The results showed an increase in the total amount of amino acids with time. Regarding aspartic and glutamic acids, their concentration increased at each stage in compost 1 and decreased in compost 2. The changes in amino acid composition were associated with variations in the composition of the microbial population. [PERSON] et al. [48] characterized the amino acid profile during the co-composting of corn straw and cattle manure.