## Results
### Metabolic Characterisation and Phylogenetic Identification of Fungal Isolates  
For fungal strains, the GenBank accession numbers for the ITS-region and for 28S rDNA sequences are MT60989-MT609917. In Figure 3, a detailed heat map with the activities produced by each fungal strain is provided. A total of 105 fungal strains were isolated during composting, but only 40 could be cultured for a long time. A total of 40 fungal strains were identified and metabolically characterised. The phylogenetic study shows high taxonomical diversity, since these isolates belonged to 23 different genera, of which most of them had a single representative. A clear succession was observed within this population with a change in the orders belonging to the early phase under semipermeable film (0, 1, 2, 5 and 8 days) corresponding to Sporidiobolales, Saccharomyces, and Agaricales. During the advanced phase (15 and 30 days), Capnoidales, Trichosporonales, and Mucorales were the most predominant. In the maturation phase (70 and 120 days), these were Hypocreales, Sordariales, and Polyporales. However, the presence of Eurotiales and Microascales was consistent throughout the whole process. Figure 2: Number of extracellular enzymes produced by fungal (**A**) and bacterial (**B**) isolates in each composting sample. Several fungi showed six activities or more, as is shown in Figure 3. Among them, strains identified as _Scopulariopsis breveiaulis_ like HM03, HM43, HM56, HM57, HT17, and HM43 showed positive results for several enzymes. Except the last one, the remaining strains showed positive results for amylolytic, pectinolytic, hemicellulose, and cellulolytic activity. The HM03 strain showed peroxidase and polyphenol oxidase activity; three of them also showed proteolytic activity. These species were isolated in different stages of the process. The six strains belonging to _S. breveiaulis_ corresponded to the bulking sample and to 30 and 70 days, _S. breveiaulis_ HT17 isolated at 55 \({}^{\circ}\)C.  
Figure 3: Heat map of the fungal isolates identified and the extracellular profile production for each isolate (blue: positive, yellow: negative). Identity (%): Percentage of nucleotide identity with the most similar GenBank sequence (Access. number (GenBank)) and order to which each sequence belongs. On the other hand, strains HM18 and HM48 were identified as _Hormographiella aspergilatta_ and showed a high number of enzyme activities, including proteases, amylases, ammoniumifying enzymes, as well as lacases, peroxididases, and polyphenol oxidase, tyrosinase, pectinases, hemicellulose, and cellulases. _Emericella nidulans_ strain HM22 produced seven enzymatic activities; among them were amyloidytic, ammoniumifying enzymes, tyrosinase, pectinase, hemicellulose, cellulase, lacase, and peroxidase. The strain _Aspergillus versicolor_ HM30 showed a high rate of production of amylase, lipase, ammoniumifying enzymes, tyrosinase, pectinases, cellulose, peroxidase, and polyphenol oxidase. In the maturation stage, _Thielavia_ sp. HM70 strain with the production of a wide set of lignocellulosic activity was remarkable. In particular, this strain showed nine enzymes, like protease, amylase, ammoniumifying enzymes and tyrosinase, pectinase, cellulase, lacase peroxidase, and polyphenol oxidase. _Bjerkandera_ sp. HM70 showed positive results in six activities, mostly oxidoreductasees like lacase, peroxidase, and polyphenol oxidase. Besides that, it showed hydrolytic activities, like cellulose, protease, and amylase. In the case of bacteria, a total of 128 strains were tested for enzyme activity production. The strains with the highest number of enzymes in each sample were selected and finally, a total of 34 bacterial strains were molecularly identified. Accession numbers for the 16S rDNA sequences are MT305959-MT305987 for bacterial strains. In this sense, representatives of each composite sample were selected according to the conditions of isolation (mesophile or thermophilic), morphotypes (colonies more frequent), and the number of enzymes produced.