## 3 Results and Discussion  
### Endocrine Disrupting Chemicals Distribution  
The Endocrine Database Exchanged (TEDX) and Database of Endocrine-Disrupting Chemicals and Their Toxicity Profile (DEDuCT) were employed for EDC identification. They comprise a list of chemicals that have shown evidence of endocrine disruption in scientific research. From the list of EDCs detected in the water samples, plastics, or those that could be part of plastics, were discarded in order to avoid an incorrect interpretation of results. As shown in Table 1, a total of 34 compounds classified as EDC were identified in surface waters of the lake (2643, 2648, 2649, 2650, 2651, 2652), evaporating pond (2635), wastewater (2575) ponds, and in springs and streams (2554, 2571, 2602, 2640, 2641, 2642). The most frequently detected EDC was cyprodini (69/112; 61.6%), followed by tonalide (44/112; 39.3%), \(O\)-phenylphenol (43/112; 38.4%), caffeine (29/112; 25.9%) and fluorene (27/112; 24.1%). Nineteen of the detected EDC can be classified as pesticides or biocides: atrazine, boscalid, carbofuran, cis-chlorgenvinphos, trans-chlorgenvinphos, cyprodini, diaz  
\begin{table}
\begin{tabular}{c c c c c c c c c c c c c c c} \hline \hline  & \multicolumn{6}{c}{**Spring and Stream**} & \multicolumn{6}{c}{**WWP**} & \multicolumn{6}{c}{**Petrola Lake**} \\ \hline
**Land Use** & **IRL** & **IRL** & **DRL** & **DRL** & **DRL** & **DRL** & **WWP** & **WWP** & **IRL** & **IRL** & **IRL** & **MP** & **LS** \\ \hline
**Control Point** & **2554** & **2571** & **2602** & **2640** & **2641** & **2642** & **2575** & **2643** & **2650** & **2649** & **2652** & **2651** & **2635** & **2648** & **Total** \\ \hline Atrazine & - & 1 & 1 & 1 & - & 1 & - & - & 1 & 1 & 1 & - & - & - & 7 \\ Carbofuran & - & - & - & - & - & - & 1 & - & - & - & - & - & - & - & 1 \\ Chlorfenvinphos, cis- & - & - & - & - & - & - & 1 & - & - & - & - & - & - & - & 1 \\ Chlorfenvinphos, trans- & - & - & - & - & - & 1 & - & - & - & - & - & - & - & - & 1 \\ Diazion & - & - & - & - & - & - & 1 & - & - & - & - & - & - & - & - & 1 \\ Dinocap & - & - & - & - & 1 & - & - & - & - & - & - & - &  
methodyml, methoprene, mevinphos, O-phenylphenol, oxadiazinon, propiconazole, propoxur, pyrimethanil, tebuconazole, terbutilazine and tetramethin; two metabolites from triazine herbicides (desethyl-atriazine and desethyl-terbutilazine); five of the identified EDC are polycyclic aromatic hydrocarbons (PAHs): acennaphthylene, dibenz[a,h]anthracene, fluoranthene, fluorene and phenantrene; four EDC compounds can be grouped as body care products, such as tonalide, butylated hydroxanisole, musk ketone and triclosan. Finally, caffeine, a legal drug, appears widespread in the different sampling points of the all water sample types. Regarding the pesticides detected, half of them (atriazine, carbofuran, chlorfenvinphos isomers, diazinon, dinocap, methoprene, mevinphos, propoxur and tetramethin) are banned in Spain and Europe. Surprisingly, clorfenvinphos, metoprene, mevinphos, tetramethin and atrazine, which were banned between 2003-2004, appear in water samples several years after banning [22; 23]. Those pesticides whose use is allowed appear more frequently than those prohibited. Cyprodinil and O-phenylphenol were the most often detected pesticides; both are broad-spectrum activity substances that can be used as pesticides in many crops and livestock, preservatives in fruits or flowers, and even as additives in the food industry [24; 25; 26]. Concerning the rest of the EDCs, there are no regulations regarding their use. However, fluoranthene (17/112; 15.2%) is a priority substance because of its negative effects on aquatic ecosystems [27]. Noticeably, tonalide (44/112; 39.3%) and caffeine (29/112; 25.9%) were the most detected EDCs; the first, tonalide, is widely used as a low-cost fragrance ingredient in cosmetics, detergents, fabric softeners, household cleaning products and air fresheners.