## 3 Results  
### UPLC-DAD-MS/MS Analysis of JGTW  
UPLC-DAD-MS/MS analysis was conducted to identify and quantify the phytochemicals in JGTW. The 24 compounds were separated on an Acquity BEH C\({}_{18}\) column (100 mm \(\times\) 2.1 mm, 1.7 \(\upmu\)m, Waters) at 40 \({}^{\circ}\)C within 22 min. The both (+)-ESI and (-)ESI modes were operated to acquire the MS spectra. The characteristics of all identified compounds in JGTW are summarized in Table 1. UV and base peak chromatograms of JGTW are presented in Figure 1A, and the extracted ion chromatograms for each phytochemical are shown in Figure 1B. Most compounds were identified in negative ion mode. The precursor ions and MS/MS fragment patterns of the phytochemicals were identified by comparisons with the mass spectra and retention times of reference standards (Supplementary Materials Figure S1). The contents of the 20 phytochemicals, except for schaffoside, isoschaftoside, neoliquiritin, and liquiritin, ranged from 0.001 to 63.971 mg/g. In EICs of several constituents (e.g., catechchin, luteolin, echinatin, glycyrrhizin) of JGTW, multiple peaks with the same _m/z_ that have different retention times were observed, considering their isomers and derivatives. Further study is required for the structural confirmation of identified constituents isolated from JGTW in detail.  
\begin{table}
\begin{tabular}{c c c c c c c c c c} \hline \hline \multirow{2}{*}{**No.**} & **RT** & **Calculated** & **Estimated** & **Error** & **Formula** & **MS/MS Fragments** & **Identifications** & **Contents** (mg/g) \\ \cline{5-10}  & **(min)** & **(\(m/z\))** & **(\(m/z\))** & **Adduct** & **(ppm)** & **Formula** & **(\(m/z\))** & **Identifications** & **Contents** (mg/g) \\ \hline \multicolumn{10}{c}{Flavonoids} \\
1 & 5.94 & 289.0718 & 289.0717 & [M\(-\)H]\({}^{-}\) & 0.377 & C\({}_{15}\)H\({}_{4}\)O\({}_{6}\) & 205.0492, 179.0334, & & & \\  & & & & & & & 125.0225 & & \\
2 & 6.91 & 593.1512 & 593.1516 & [M\(-\)H]\({}^{-}\) & 1.019 & C\({}_{27}\)H\({}_{30}\)O\({}_{15}\) & 473.1066, 413.0872, & & & \\  & & & & & & 383.0770, 354.0663 & & & \\
3 & 7.63 & 563.1406 & 563.1406 & [M\(-\)H]\({}^{-}\) & 0.316 & C\({}_{26}\)H\({}_{28}\)O\({}_{14}\) & 473.1069, 443.0968, & & & \\  & & & & & 383.0760, 353.0656 & & & & \\
4 & 8.65 & 419.1337 & 419.1338 & [M+H]\({}^{+}\) & 0.308 & C\({}_{21}\)H\({}_{22}\)O\({}_{9}\) & 257.0808 & & & \\
5 & 8.80 & 417.1191 & 417.1192 & [M\(-\)H]\({}^{-}\) & 0.512 & C\({}_{21}\)H\({}_{22}\)O\({}_{9}\) & 417.1173, 255.0651, & & & \\  & & & & & 135.0069, 119.0481 & & & & \\
6 & 8.93 & 303.0510 & 303.0509 & [M\(-\)H]\({}^{-}\) & 0.287 & C\({}_{15}\)H\({}_{2}\)O\({}_{7}\) & 285.0392, 275.0558, & & & \\  & & & & & & 2959.0611, 217.0493, & & & & \\  & & & & & & 177.0177, 125.0224 & & & & \\
7 & 11.92 & 255.0663 & 255.0660 & [M\(-\)H]\({}^{-}\) & 0.136 & C\({}_{15}\)H\({}_{2}\)O\({}_{4}\) & 255.0652, 135.0069, & & & \\  & & & & & & & 119.0483 & & & \\
8 & 12.29 & 285.0405 & 285.0405 & [M\(-\)H]\({}^{-}\) & 0.022 & C\({}_{15}\)H\({}_{6}\)O\({}_{6}\) & 217.0493, & & & \\  & & & & & & 135.0433 & & & \\
9 & 12.53 & 285.0758 & 285.0760 & [M+H]\({}^{+}\) & 0.731 & C\({}_{16}\)H\({}_{2}\)O\({}_{5}\) & 285.0757, 270.0525 & & & \\
10 & 13.91 & 271.0612 & 271.0612 & [M\(-\)H]\({}^{-}\) & \(-\)0.158 & C\({}_{15}\)H\({}_{2}\)O\({}_{5}\) & 271.0641, 177.0177, & & & \\  & & & & & 165.0181, 151.0019 & & & & \\
11 & 14.23 & 285.0405 & 285.0403 & [M\(-\)H]\({}^{-}\) & 0.343 & C\({}_{15}\)H\({}_{6}\)O\({}_{6}\) & 285.0396, 167.0332, & & & \\  & & & & & & 109.0275 & & & \\
12 & 16.28 & 269.0808 & 269.0809 & [M+H]\({}^{+}\) & 0.344 & C\({}_{16}\)H\({}_{2}\)O\({}_{4}\) & 269.0807, 254.0577, & & & \\  & & & & & & 237.0548 & & & \\  & & & & & & & & 237.0548 & & \\  & & & & & & & & & \\  & & & & & & & & & & \\  & & & & & & & & & & \\  & & & & & & & & & \\  & &