Furthermore, they can be used to relatively quickly monitor static or dynamic metabolic changes [27; 30; 31; 32]. Other important uses of fungal VOCs include biotechnological processes [30; 33; 34], signalling of ecological communication between different species [31; 35], and obtaining information about fungal development [36; 37]. The high diversity of VOCs released by bacteria and fungi justifies the creation of a database that summarizes the taxonomic distribution of around 2000 compounds and their possible functions. The first version of the mVOC Database was created in 2014, and was renewed this year [38; 39]. No volatile compounds of _Malassezia_ have been reported in this database, because the data sources used have been limited to PubMed publications [39]. The first and only description of _Malassezia_ volatiles, in which the authors reported the presence of 11 different VOCs, was performed in 1979, when the genus was recognized as _Pityrosporum_[40]. The human skin sebum contains a complex mixture of triglycerides, fatty acids, wax esters, sterol esters, cholesterol, and squalene. Among the fatty acids, the unsaturated ones predominate,but the sebum is generally composed of OA, PA, stearic acid, linoleic acid, sapienic acid, and palmitoleic acid [41]. OA is an irritant component that can induce dandruff in dandruff-susceptible people [10; 41]. Among animals, PA is a fatty acid found in the human sebum in a relatively high amount [21]. Having this in mind, the aim of this study is to investigate how the lipid composition of a liquid medium influences the production of VOCs released by _M. furfur_, the only _Malassezia_ species known to survive in the PA medium. To accomplish this, four different media for growing the strain CBS 1878 were prepared: (1) mDixon, (2) minimal medium (MM) supplemented with oleic acid (OA), (3) MM with oleic + palmitic acid (OA+PA), and (4) MM with palmitic acid (PA). The VOCs released by the yeast grown in these media were analyzed using headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). The mDixon medium was selected as the reference for in vitro growing conditions. The other media serve to identify the VOCs released under restricted nutritional conditions when an unsaturated acid (OA), a saturated acid (PA), or a combination of both is present in the medium. In addition, samplings of VOCs were performed for each medium in the exponential and stationary phase. Finally, results from eight experimental treatments (mDixon in both growing phases: De, Ds; oleic acid in both growing phases: Oe, Os; oleic + palmitic acid in both growing phases: Ope, OPs; and, finally, palmitic acid in both growing phases: Pe, Ps) were obtained. Previous reports on other species have demonstrated that changes in the microbial growth conditions produce qualitative and quantitative alterations in the VOCs released by an organism [28; 42; 43]. Based on these findings, we hypothesized the existence of a chemical differentiation in the VOCs profiles released by _M. furfur_ CBS 1878 due to the different lipid composition of the medium and to the phase of growth. Univariate comparisons and unsupervised and supervised multivariate analyses have been applied to measure the metabolic differentiation and its significance.