## 3 Results
### GO Annotation and KEGG Enrichment of Differentially Expressed Genes in G. sinensis
\begin{table}
\begin{tabular}{l c c c c c} \hline \hline \multicolumn{1}{c}{\multirow{2}{*}{**Pathway**}} & \multicolumn{5}{c}{**Gene Numbers**} \\ \cline{2-6}  & **GS2 vs. GS1** & **GS3 vs. GS1** & **GS4 vs. GS1** & **GS5 vs. GS1** & **GS6 vs. GS1** \\ \hline Alanine, aspartate, and glutamate metabolism & 41 ** & 54 * & 62 ** & 56 ** & 74 ** \\ alpha-Linolenic acid metabolism & 27 * & 52 * & 56 ** & 46 * & 67 ** \\ Amino sugar and nucleotide sugar metabolism & 51 & 125 * & 126 ** & 108 * & 150 ** \\ Anthocyanin biosynthesis & 9 ** & 4 & 5 * & 7 ** & 7 ** \\ Ascorbate and aldarate metabolism & 63 ** & 91 ** & 93 ** & 93 ** & 96 ** \\ Biosynthesis of secondary metabolites & 742 ** & 1129 ** & 1113 ** & 1054 * & 1347 ** \\ Biosynthesis of unsaturated fatty acids & 40 & 18 & 21 & 14 & 40 * \\ Brassinosteroid biosynthesis & 26 & 45 & 46 & 8 & 12 * \\ Butanoate metabolism & 19 ** & 21 & 20 & 14 & 16 \\ Carbon fixation in photosynthetic organisms & 55 ** & 87 & 91 * & 86 * & 110 ** \\ Carotenoid biosynthesis & 60 ** & 85 ** & 72 ** & 83 ** & 99 ** \\ Cutin, suberin, and wax biosynthesis & 36 ** & 49 ** & 49 ** & 45 ** & 66 ** \\ Cyanoamino acid metabolism & 35 * & 48 & 52 & 55 & 84 ** \\ Cysteine and methionine metabolism & 30 & 75 & 93 ** & 76 * & 94 ** \\ Diterpenoid biosynthesis & 39 ** & 45 ** & 49 ** & 39 ** & 47 ** \\ DNA replication & 37 * & 75 ** & 67 * & 70 ** & 67 \\ Endocytosis & 80 & 216 ** & 228 ** & 212 ** & 244 ** \\ Ether lipid metabolism & 61 & 134 * & 151 ** & 138 ** & 166 ** \\ Fatty acid metabolism & 9 & 63 & 63 & 45 & 63 * \\ Flavone and flavonoid biosynthesis & 82 ** & 76 ** & 64 ** & 63 ** & 89 ** \\ Flavonoid biosynthesis & 132 ** & 152 ** & 148 ** & 145 ** & 172 ** \\ Fructose and mannose metabolism & 3 & 48 & 41 & 50 & 80 * \\ Glucosinolate biosynthesis & 6 & 10 & 15 ** & 12 * & 14 * \\ Glycoslymphostatidylinositol anchor & 6 & 18 ** & 20 ** & 19 ** & 17 * \\ Biosynthesis & 9 * & 7 & 6 & 4 & 5 \\ Glyoxylate and dicarboxylate metabolism & 44 ** & 16 & 13 & 57 ** & 80 ** \\ Indole alkaloid biosynthesis & 9 ** & 14 ** & 11 * & 10 * & 16 ** \\ Limonene and pinee degradation & 54 ** & 74 ** & 73 ** & 60 ** & 61 ** \\ Metabolic pathways & 1117 ** & 1967 ** & 2018 ** & 1871 ** & 2293 ** \\ Monoterpool biosynthesis & 13 ** & 77 & 80 & 24 ** & 27 ** \\ N-Glycan biosynthesis & 28 * & 47 & 47 & 44 & 62 ** \\ Other glycan degradation & 45 ** & 73 ** & 77 ** & 73 ** & 81 ** \\ Pentose and glucuronate interconversions & 56 ** & 130 ** & 136 ** & 129 ** & 146 ** \\ Phagosome & 29 & 7 & 8 & 68 & 108 * \\ Phenylalanine metabolism & 61 ** & 90 ** & 90 ** & 82 ** & 88 ** \\ Phenylalanine, tyrosine, and tryptophan biosynthesis & 33 ** & 60 ** & 59 ** & 46 ** & 57 ** \\ Phenylpropanoid biosynthesis & 153 ** & 189 ** & 187 ** & 191 ** & 235 ** \\ Photosynthesis & 82 ** & 65 ** & 94 ** & 72 ** & 72 ** \\ Photosynthesis--antenna proteins & 60 ** & 40 ** & 68 ** & 62 ** & 45 ** \\ Plant hormone signal transduction & 242 ** & 648 ** & 683 ** & 602 ** & 664 ** \\ Plant–pathogen interaction & 214 & 843 ** & 904 ** & 734 ** & 700 ** \\ Porphyrin and chlorophyll metabolism & 49 ** & 50 & 68 ** & 63 ** & 65 * \\ Propanoate metabolism & 28 * & 8 & 9 & 43 & 45 \\ Pyruvate metabolism & 42 & 101 ** & 73 & 88 ** & 117 ** \\ Sesquiterpenoid and triterpen biosynthesis & 23 ** & 38 & 35 & 20 & 28 * \\ Starch and sucrose metabolism & 114 ** & 244 ** & 252 ** & 223 ** & 301 ** \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Differentially expressed unigene numbers of KEGG enrichment pathways in _G. sinensis_.