## 3 Results
### Functional Annotations  
Once phylogeny analysis was completed, we proceeded with the secondary metabolism cluster prediction of strain L5B5 based on antiSMASH tool, where _P. piscis_ as well as the three strains of _P. protegens_ were included due to their tight-knit relationship. Quantitatively, the secondary metabolism was more dynamic in strain L5B5 with the prediction of 20 gene clusters, followed of _P. piscis_ with 18, _P. protegens_ Pf-5, with 16, and _P. protegens_ strains CHA0  
Figure 5: Biological processes annotations for _Pseudomonas_ sp., strain L5B5 and complete genome representation. Figure 6: Pairwise genome comparison of _Pseudomonas_ sp., strain L5B5, and closest relatives by means of Average Nucleotide Identity using BLAST (ANID) and MUMmer (ANIm). 1: _Pseudomonas_ sp. strain L5B5; _2: P. piscis_ MC042; 3: _P. protegens_ Cab57; 4: _P. protegens_ Pf-5; 5: _P. protegens_ CHA0; 6: _P. moorei_ RW10; 7: _P. rhizosphere_ DSM 16299; 8: _P. molini_ DSM 18327; 9: _P. lanyulsulfitorans_ AP3 22; 10: _P. baetica_ a390. and Cab57, with 15 each (Table 1), although it is necessary to consider that antiSMASH divides pyoverdine metabolite in two separated gene clusters. Every predicted gene cluster was blasted against UniProtKB database of UniProt to find a closest approximation for functional annotation. Strain L5B5 shared common secondary metabolites, also called core metabolites, with relatives, such as the mentioned pyoverdine, oramide biosurfactants, the antimicrobial pyrrolnirin, fengycin, and 2,4-diacetylphyloroglucinol, as well as unknown and unspecified secondary metabolism-like gene clusters. Genes involved in the synthesis of pyoluteorin and the pyochelin-type siderophores were not observed in _P. piscis_. The production of phenazines were predicted exclusively in strain L5B5 and _P. piscis_. Non-ribosomal peptide synthetase-type clusters 13 and 19, one siderophore in region 11, as well as one homoserine lactone and one Aryl polyene cluster, in region 14 and 15, respectively, were unique in strain L5B5. UniProt similarity using BLAST for strain L5B5 resulted in a major relatedness of genes cluster with undescribed and described _Pseudomonas_, especially with _Pseudomonas_ sp. CMR5c and CMR12a as well as with _P. piscis_ and _P. protogens_ CHA0. The study of resistance by means of CARD database concluded with the prediction of a major number of genes detected in the strain L5B5 in relation to the closest relatives. A total of seven \"Strict\" hits were found, two more than those observed in _P. piscis_ and  
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three more than in the three _P. protegens_ strains. \"Strict\" hits identified unknown variants of known antimicrobial resistance mechanisms (ARM) with curated similarity threshold to ensure that detected variants describe a functional antimicrobial gene. All hits found in the analyzed bacteria identified the same Antibiotic Resistance Ontology (ARO) terms, which define the annotation of the ARM [49] with the detection criteria \"protein homolog model\". The hit ARO:3005040, which describes the interaction of YajC protein with the AcrAB-TolC efflux pump, conferring resistance to certain \(\beta\)-lactam, chloramphenicol, and tetracycline antibiotics, was found in the strain L5B5 and the other related bacteria. The other six hits detected matched with the ARO:3000777, which defines the role of AdeF, the membrane fusion protein of the multidrug efflux complex AdeFGH, to confer resistance to tetracycline and fluoroquinoline antibiotics. For _Pseudomonas_ sp., strain L5B5, the analysis of annotations of virulence factors (VF) with VFDB derived in the prediction of 185 known or potential genes involved in these mechanisms. In _P. piscis_, 233 genes were predicted, whereas 178 were found in _P. protegens_ strains CHA0 and Pf-5 and 175 hits in _P. protegens_ Cab57. VFDB found homology with described VF in _P. aeruginosa_ strains PA7, PAO1 and UCBPP-PA14, _P. fluorescens_ strains Pf0-1 and SBW25, _P. protegens_ Pf-5, _P. putida_ KT2440, _P. syringae_ pv.