## 2 Results and Discussion
### In Vitro Cultures
(2017), where \(\frac{1}{2}\) MS medium with the addition of different BAP and KIN concentrations was used. At a later stage of the experiment, the passage was changed to full MS medium with the addition of various concentrations of GA3 (gibberellic acid) to initiate elongation. The best results were obtained with a GA3 concentration of 1.0 mg-dm\({}^{-3}\). In the next phase, rooting was carried out, which was the most effective on medium containing 1.5 mg-dm\({}^{-3}\) IBA. Nodal explants were also used by [PERSON] et al. (2011) [PERSON] et al. (2017). The highest rate of shoot regeneration was obtained on MS medium with the addition of 0.5 mg-dm\({}^{-3}\) ZEA (zeatin) and 0.5 mg-dm\({}^{-3}\) IAA, root regeneration on MS with 1.0 mg-dm\({}^{-3}\) BAP and 1.0 mg-dm\({}^{-3}\) IAA, while the elongation increase was most effective on MS with 0.5 mg-dm\({}^{-3}\) ZEA and 0.5 mg-dm\({}^{-3}\) IAA. During our study of average root length, it was also noted that the lowest result for individual explants was obtained on MS-3 medium (0.5 mg-dm\({}^{-3}\) IBA)-this result was less than half the length of  
\begin{table} \begin{tabular}{c c c c c c c c} \hline \hline \multirow{2}{*}{**Medium**} & **Explant** & **Plant Height** & **No. of New** & **No. of New** & **Length of the** & **Length of the** & **Average Root** \\  & **Type** & **(cm)** & **Nodal** & **lateral** & **Smallest Lateral** & **Largeest Lateral** & **Length (cm)** \\  & & & **Segments** & **Shoots** & **New Shoots (cm)** & **New Shoots** & **L** & **New Shoots** \\ \hline MS-K & & \(11.5\pm 0.16\,^{\circ}\) & \(5.1\pm 0.21\,^{\circ}\) & \(1.9\pm 0.12\,^{\circ}\) & \(2.2\pm 0.20\,^{\circ}\) & \(5.0\pm 0.60\,^{\circ}\) & \(3.5\pm 0.15\,^{\circ}\) \\ \hline MS-1 & Aptical & \(5.8\pm 0.20\,^{\circ}\) & \(4.1\pm 0.36\,^{\circ}\) & \(1.8\pm 0.54\,^{\circ}\) & \(2.3\pm 0.15\,^{\circ}\) & \(2.8\pm 0.97\,^{\circ}\) & \(3.4\pm 0.24\,^{\circ}\) \\ MS-2 & meristem & \(6.7\pm 0.13\,^{\circ}\) & \(4.9\pm 0.06\,^{\circ}\) & \(1.8\pm 0.54\,^{\circ}\) & \(3.1\pm 0.24\,^{\circ}\) & \(4.1\pm 1.41\,^{\circ}\) & \(3.0\pm 0.29\,^{\circ}\) \\ MS-3 & \(10.1\pm 0.12\,^{\circ}\) & \(5.5\pm 0.67\,^{\circ}\) & \(2.6\pm 0.57\,^{\circ}\) & \(2.6\pm 0.14\,^{\circ}\) & \(4.1\pm 0.29\,^{\circ}\) & \(1.9\pm 0.07\,^{\circ}\) \\ MS-4 & \(6.5\pm 0.15\,^{\circ}\) & \(6.4\pm 0.57\,^{\circ}\) & \(2.2\pm 0.73\,^{\circ}\) & \(2.2\pm 0.19\,^{\circ}\) & \(3.3\pm 1.26\,^{\circ}\) & \(4.2\pm 0.17\,^{\circ}\) \\ \hline Average & & \(8.1\pm 2.31\,^{\circ}\) & \(4.9\pm 0.61\,^{\circ}\) & \(2.1\pm 0.54\,^{\circ}\) & \(2.5\pm 0.40\,^{\circ}\) & \(3.9\pm 1.13\,^{\circ}\) & \(3.4\pm 0.77\,^{\circ}\) \\ \hline MS-K & Nodal & \(6.9\pm 0.23\,^{\circ}\) & \(5.0\pm 0.32\,^{\circ}\) & \(1.4\pm 0.29\,^{\circ}\) & \(4.4\pm 0.13\,^{\circ}\) & \(4.6\pm 0.23\,^{\circ}\) & \(4.7\pm 0.23\,^{\circ}\) \\ MS-1 & segment & \(6.4\pm 0.10\,^{\circ}\) & \(4.7\pm 0.55\,^{\circ}\) & \(2.3\pm 1.01\,^{\circ}\) & \(3.3\pm 0.09\,^{\circ}\) & \(4.5\pm 0.38\,^{\circ}\) & \(5.2\pm 0.14\,^{\circ}\) \\ MS-2 & & \(7.7\pm 0.25\,^{\circ}\) & \(5.5\pm 0.21\,^{\circ}\) & \(2.9\pm 0.81\,^{\circ}\) & \(3.6\pm 0.10\,^{\circ}\) & \(5.2\pm 0.25\,^{\circ}\) & \(7.0\pm 0.23\,^{\circ}\) \\ MS-3 & \(7.2\pm 0.13\,^{\circ}\) & \(6.3\pm 0.61\,^{\circ}\) & \(1.4\pm 0.30\,^{\circ}\) & \(4.5\pm 0.20\,^{\circ}\) & \(4.9\pm 0.60\,^{\circ}\) & \(4.3\pm 0.28\,^{\circ}\) \\ MS-4 & \(7.8\pm 0.15\,^{\circ}\) & \(6.1\pm 0.40\,^{\circ}\) & \(2.2\pm 0.78\,^{\circ}\) & \(2.8\pm 0.12\,^{\circ}\) & \(4.6\pm 0.88\,^{\circ}\) & \(6.0\pm 0.31\,^{\circ}\) \\ \hline Average & & \(7.2\pm 0.54\,^{\circ}\) & \(5.5\pm 0.73\,^{\circ}\) & \(2.0\pm 0.84\,^{\circ}\) & \(3.7\pm 0.68\,^{\circ}\) & \(4.8\pm 0.58\,^{\circ}\) & \(5.4\pm 1.01\,^{\circ}\) \\ \hline \hline \end{tabular}