## 1 Introduction  
The side effects associated with the administration of chemotherapeutic medication are currently regarded as an issue of major concern in chemotherapy [1]. Both cytotoxic and immunomododulatory chemotherapy cause myelosuppression and immunosuppression, which degrade body functions and reduce life quality [2]. Long-term chemotherapy can also contribute to the development of immunodeficiency, which can enhance the susceptibility to infections and reduce cancer immunosuppressiveillance [3]. Although certain immunomododulatory drugs can be used to prevent these side effects, these too are known to have associated adverse effects, including fever, flu-like symptoms, and allergic reactions [4]. In contrast, natural herbal preparations are considered potentially effective immunomododulators that have little or no adverse effects [5].  
Hemomine (HM; \(\sim\)60% solids, LJG305) is a modified version of Samul-Tang, also known as Siwu-Tang, a well-known herbal blend used for treating hematological disease that consists of the same amount of four medicinal herbs containing root extracts from Angelicae Gigantis Radix, _Cridium officinal_ [PERSON], _Paeonia lactiflora_ [PERSON], and _Rehmannia glutinosa_ [PERSON] [6]. Hemomine additionally contains _Astragalus membranacus_and _Glycyrrhiz uralensis_[7]. Among these constituents, the predominant component, Angelicae Gigantis Radix (3.9%), contains a range of bioactive chemicals, including nodakenin, decursin, decursinol angelate, and decursinol [8]. Previous studies have examined the immunodulatory effects of Angelicae Gigantis Radix on cyclophosphamide (CPA)-induced immunosuppression [9]. Furthermore, the aforementioned bioactive components of Angelicae Gigantis Radix have been established to have anti-inflammatory activities [10; 11; 12; 13]. Similar anti-inflammatory and immunomododulatory effects have been demonstrated for other herbs in the HM preparation: _A. membranacueus_[14], _P. lactiflora_[15; 16], _C. officinal_ Makino [17], and _G. uralensis_[18; 19]. However, despite the fact that herbal formulations are assumed to be more synergistic than individual immunomododulatory herbal substances, there is a lack of research on the effects of these immunomododulatory herbal mixes on immunological modulation. Therefore, it is anticipated that the combination of these herbs in HM would have a more pronounced immunomododulatory effect.  
Cyclophosphamide is a well-known alkylating chemical that inhibits cell division by promoting cross-linking in DNA and inhibiting the proliferation of neoplastic cells [20]. This cytotoxic drug is used to treat a range of autoimmune diseases and cancers, including lymphoma, myeloma, and chronic lymphocytic leukemia [21]. However, CPA therapy has been reported to have adverse effects on hematological parameters and lymphoid organs, thereby resulting in myelosuppression and immunosuppression [22; 23]. Consequently, CPA is also utilized in myelosuppressive and immunosuppressive animal models to investigate the immunomododulatory effects of natural compounds [24; 25].  
In this study, we investigated the immune-enhancing effects of HM on CPA-induced myelosuppression and immunosuppression in mice, which have been employed as a useful animal model for evaluating the immunomodulatory effects of natural substances. Given that each of the constituent herbs comprising HM has potent anti-inflammatory properties, we hypothesized that this herbal blend would have excellent immunomodulatory properties. To verify this assumption, we used model mice to determine changes in body weight, immune organ indices, hematological parameters, serum and splenic cytokines levels, and natural killer (NK) cell activities, and also examined lymphoid organ histopathology.