## Materials and Methods
### Data Analysis  
[PERSON]'s condition index (K) was determined from the biometric data, according to the following formula [50]:  
\[\text{K}=100\;(\text{W/L}^{3}), \tag{1}\]  
where W is the animal weight (g) and L is the total length (cm). Mercury net accumulation rates (NAR; mg\(\cdot\)kg\({}^{-1}\cdot\)day\({}^{-1}\)) for each tissue and treatment were determined after 14 and 28 days of exposure, as follows [51]:  
\[\text{NAR}_{\text{t}}=([\text{THg}_{\text{t}}]\text{ tissue/t}), \tag{2}\]  
where [THg\({}_{\text{t}}\)] is the average total Hg concentration in fish tissue after 14 and 28 days of exposure. The THg elimination factor (EF) after seven days of elimination (i.e., at day 35 of the trial) was calculated according to the following formula:  
\[\text{EF}\;(\%)=100-((\text{CDay35/CDay28})\times 100), \tag{3}\]  
where CDay35 is the average concentration (mg\(\cdot\)kg\({}^{-1}\) wet weight (ww)) in the fish tissue from each contaminated treatment at day 35 (elimination phase), and CDay28 is the average concentration (mg\(\cdot\)kg\({}^{-1}\) ww) in the fish tissue at the end of the exposure period [52]. Considering that, after seven days of elimination, EF values were approximately or below zero in the brain and muscle, only the EF values obtained in the liver were considered in data analysis. The percentage of Hg tolerable weekly intake (TWI) achieved with the consumption of contaminated fish species was estimated under the present and future climate conditions, using the empirical data acquired in the present study, as well as data reported in previous studies using two ecologically distinct pelagic species (_Argyrosomus regius_[25] and _Dicentrachus labrax_[43]). To do so, the TWI value of 4 ug\(\cdot\)kg\({}^{-1}\) body weight (bw) [4] was used, and a weekly consumption of 150 g of fish by adults with an average body weight, bw, of 70 kg [4] was considered. Values were presented as percentage change in the contribution of Hg TWI (\(\%\)) accomplished with the consumption of contaminated fish (muscle) reared under climate change effects in relation to normal T and pH conditions. The hazard quotient (HQ) is frequently used to calculate the risk of a certain population to the exposure of a particular contaminant. To this purpose, values were determined according to the following equation [53]:  
\[\text{HQ}=(\text{EF}\times\text{ED}\times\text{FIR}\times\text{C})/(\text{RfD }\times\text{WAB}\times\text{TA})\times 10^{-3}, \tag{4}\]  
where EF denotes exposure frequency (365 days/year), ED is the exposure duration (70 years), FIR is the fish ingestion rate (seafood: 61.55 g/capita/day [54]), C is Hg concentration in seafood (ug\(\cdot\)g\({}^{-1}\), determined or predicted, according to the scenarios), RfD is the oral reference dose (\(5\times 10^{-4}\) ug\(\cdot\)g\({}^{-1}\cdot\)day\({}^{-1}\) for Hg [53]), WAB is the average body weight (70 kg), and TA is the average exposure time for non-carcinogens (365 days \(\times\) ED). HQ values \(<\)1 imply that the exposed population is unlikely to experience obvious adverse effects, while HQ \(>\)1 mean that the possibility of harmful effects to human health cannot be excluded [53]. The HQ baseline reference was calculated according to Hg concentrations registered for both demersal (sole [55], flounder and mullet [2], halibut [56], Atlantic cod [57], and herring [58]) and pelagic wild species (meagre [59], seabass [60], black scabbard fish [12], bluefin tuna [3], fresh tuna, tope-shark [61], and Atlantic mackerel [62]). This baseline was compared with the estimated HQ under climate change scenarios (warming, acidification, and both warming and acidification) for demersal species, assessed according to the percentage variation for each scenario in S. _senegalensis_ (this study). For pelagic species, the variation encountered for _A. regius_ was considered [25]. Statistical analysis was conducted at a significance level of 0.05, using the STATISTICA data analysis software system (version 8.0., StatSoft, Inc., 2007, Tulsa, OK, USA).