## 4 Discussion  
Preliminary results obtained in the first set of experiments clearly demonstrate the existence of distant interactions between water samples and HD Abs to IFNy under CMF  
Figure 6: Maximum chemiluminescence intensity in a suspension of neutrophils after the addition of water preincubated with HD PMA in different magnetic fields. The samples contained: suspension of neutrophils—2.5 \(\times\) 10\({}^{5}\) cells; water sample incubated for 0 min (empty bar) and 60 min (hatched bar) at: SMF (60 uT), \"0”MF (“magnetic vacuum” \(\sim\)-20 nT), CMF (12.6 Hz)—combination of static (60 uT) and alternating magnetic field (100 nT), which is formed according to the sinusoidal regime at 12.6 Hz, CMF (48.5 Hz)—combination of SMF (60 uT) and AMF (100 nT), which is formed according to the sinusoidal regime at 48.5 Hz, GMP—outside the controlled magnetic field generating device; fMLF (2 uM); luminol (0.35 mM). The acceptor (water) accounted for 80% of the total volume of the sample. Measurements were performed at 37 \({}^{\circ}\)C and pH 7.4. For all pairwise comparisons within the group “Water after 60 min incubation with HD PMA” \(p<0.05\); for all comparisons of corresponding samples between groups “Water after 60 min incubation with HD PMA” and “Water (control)” \(p<0.05\) (except samples which were incubated in “0” MF. For these * \(p>0.05\)). conditions, since the presence of HD Abs to IFNy near water modifies its activity. This was done in comparison with the magnetic field treatment of water samples located separately from HD Abs to IFNy. This phenomenon of distant interaction of samples with each other was even more pronounced when instead of water, an IFNy solution was jointly incubated with HD Abs to IFNy. However, it should be noted that in these experiments, the activity of the samples was compared with only one time point--the value at \"0 minutes\" of joint incubation of samples. Additionally, the effects of other factors such as the absorption of carbon dioxide from the atmosphere could have influenced the obtained results. In this regard, in the main group of experiments we adjusted the experimental conditions: we unified the incubation time of experimental and control samples and used a comparison of samples incubated under different conditions of magnetic field and with shielding. In all experiments, we used the same incubation time (60 min) and also used samples with tightly closed lids to reduce possible absorption effects. It should be noted that after these adjustments, the distant effect of HD preparations was reliably detected. It should also be clarified that the GMF mode (incubation outside the device) differs from the SMF not only by a slightly lower CMF (45 \(\upmu\)T), but also by the presence of relatively low-intensity variable fields (mainly at an industrial frequency of 50 Hz, \(\sim\)50 nT), which are shielded in the device. For the studies described in this paper, we opted for CMF (combination of SMF (60 \(\upmu\)T) and AMF (100 nT), which is formed according to the sinusoidal regime at 12.6 Hz), because the stability of the measurement results when applying this field is higher compared to the much more efficient but less stable CMF mode, where the modulation is carried out according to the sinusoidal regime at 48.5 Hz. The results obtained in our studies agree with the previously described emission of HD samples in the IR range, as well as changes in the parameters of the hydrogen bond network in aqueous solutions of IFNy after joint incubation with HD Abs to IFNy[14; 22]. It has been shown that the samples of HD Abs to IFNy themselves not only emit in the IR range, but may contribute to a change in the IR emission spectra of the IFNy solution after its joint incubation with HD Abs to IFNy[14]. Another study, performed using a similar model, shows a change in the parameters of the THz spectrum of the IFNy solution which was incubated in the presence of HD Abs to IFNy.