## 3 Results  
Fungal colonies were developed rapidly on Sabouraud media, from samples taken before the application of EOs. At 72 h of incubation, the first fungal colonies could be macroscopically visualised. After 10 days of incubation, the plates were completely invaded. Figure 3 shows the plates on the seventh day of incubation; each image indicates the examined area of the sheepskin coat. The high degree of fungal contamination of the coat can easily be observed. In the above-mentioned way, seven different types of colonies were identified [80,81], with six being from the mould class (_Alternaria_ sp., _Aspergillus_ sp., _Botrytis_ sp., _Cladosporium_ sp., _Mucor_ sp. and _Penicillium_ sp.) and one from the yeast class (_Candida guillermondii_) (Table 2). As previously stated the number of samples taken after applying the EOs was 3 sets of 6, taken 30 min, 24 h and 48 h after applying the corresponding EO. The plates were monitored for 30 days in order to evaluate the inhibitory effects of the EOs. Figure 4 shows the plates, as read on day 14 of the incubation. The inhibitory effects of the EOs are evident. The only plate on which a fungal colony developed is the one corresponding to the inner area of the sheepskin coat, present in the sample taken 48 h after applying the EO of _Melaleuca alternifolia_ (tea tree). The isolated fungal colony consisted of _Cladosporium_ sp., which became macroscopically visible on day 5 of incubation. It was observed that this type of mould was not isolated from the primary sample, as is detailed in the discussion section below. Figure 3: Plates seeded with primary samples (before application of essential oils). Reading taken after 7 days of incubation (**A**)—_Lavandula angustifolia_; (**B**)—_Cistrus lemon_; (**C**)—_Mentha pipertia_; (**D**)—_Melaleuca alternifolia_; (**E**)—_Marioram_; (**F**)—_Origanum vulgare_. \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Microscopic Image** & **Genus Name** &
\begin{tabular}{c} **The Examined Area of the** \\ **Sheepskin Coat from Which** \\ **It Was Isolated (Figure 2)** \\ \end{tabular} & **Applied Essential Oils—Effect** \\ \hline \hline _Alternaria_ sp. & Area E & _Marjoram_—inhibitory \\ \hline _Aspergillus_ sp. & Area E & _Marjoram_—inhibitory \\ \hline \hline _Apergillus_ sp. & Area E & _Marjoram_—inhibitory \\ \hline \hline _Apergillus_ sp. & Area A & _Lavandula angustifolia_—inhibitory \\ \hline _Apergillus_ sp. & Area E & _Citrus limon_—inhibitory \\ \hline _Apergillus_ sp. & Area C & _Menthu piperta_—inhibitory \\ \hline _Apergillus_ sp. & Area E & _Marjoram_—inhibitory \\ \hline \hline _Candida guillermodii_ & Area D & _Melaleuca alternifolia_—inhibitory \\ \hline \hline _Candida guillermodii_ & Area D & _Melaleuca alternifolia_—inhibitory \\ \hline \hline _Candida guillermodii_ & Area D & _Melaleuca alternifolia_—inhibitory \\ \hline \hline _Candida guillermodii_ & Area D & _Melaleuca alternifolia_—inhibitory \\ \hline \hline _Candida guillermodii_ & Area D & _Melaleuca alternifolia_—inhibitory \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Identified fungi and the effect of essential oils. Figure 5a-c show the plates, as read on day 30 of the incubation. The developed colonies became macroscopically visible after between 20 days to 25 days of incubation. The results, obtained after the samples from the examined areas were examinedat 30 min, 24 h and 48 h following the application of the EOs, are presented in Table 3.  
The existing difference was due to the duration of the effect that they had on certain types of fungi as shown on Table 4. It is evident that some EOs (e.g., _Lavandula angustifolia, Melaleuca alternifolia, Marjoram, Origanum vulgare_) inhibited the action of some fungal genera up to a period of 22 days, whereas on others the effect lasted for a period of at least 30 to 32 days (Table 4).