## 3 Results
### Optimization the Parameters and Verification of Design in US Processing  
The responses obtained from 18 experimental points with US pasteurization according to the CCD are shown in Table 4. For 18 experimental points, the values of TPC, turbidity, \"Brix, color a*, and yeast and mold count ranged from 1079 to 1481 mg GAE/L, 3.88 to 4.71 NTU, 14 to15 \({}^{\circ}\)Bx, 3.03 to 3.88, and \(<\)0.5 to 4.3 log CFU/mL, respectively. ANOVA analysis of the data obtained from the experimental design and the interaction of the temperature, US time, and US power on/off position on the responses for yeast and mold count of samples are shown in Table 5. The results showed that for the yeast and mold content, temperature, time, and ultrasound on/off parameters were statistically significant. Increasing the temperature and processing time reduced yeast and mold count. US power-off which means no ultrasound treatment was used as a control to figure out the effect of the combination of temperature and treatment time used with US power-on position on the results. Under the US power-off condition, temperature application decreased yeast and mold count. Providing the US power-on position and increasing temperature, that process condition made a significant contribution to yeast and mold reduction (\(p<0.05\)). \begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline \multirow{2}{*}{**Run Order**} & \multirow{2}{*}{\(\mathbf{X_{1}}\)} & \multirow{2}{*}{\(\mathbf{X_{2}}\)} & \multicolumn{2}{c}{**Phenolic**} & & & **Yeast and Mold** \\  & & & & **Content** & & **Turbidity** & \({}^{\circ}\)**Brix** & **Color a* & **Count log** \\  & & & & **(mg GAE/L)** & & & & **CFU/mL** \\ \hline
1 & 50 & 10 & ON & 1252 \(\pm\) 1.81  & 4.23 \(\pm\) 0.000  & 15 \(\pm\) 0.0  & 3.46 \(\pm\) 0.00  & 3.4 \(\pm\) 0.67  \\
2 & 40 & 10 & OFF & 1103 \(\pm\) 0.27  & 4.11 \(\pm\) 0.003  & 15 \(\pm\) 0.0  & 3.46 \(\pm\) 0.01  & 4.4 \(\pm\) 0.80  \\
3 & 50 & 15 & ON & 1273 \(\pm\) 0.18  & 4.24 \(\pm\) 0.011  & 15 \(\pm\) 0.0  & 3.46 \(\pm\) 0.00  & 3.0 \(\pm\) 0.96  \\
4 & 40 & 15 & ON & 1138 \(\pm\) 0.18  & 4.20 \(\pm\) 0.003  & 15 \(\pm\) 0.0  & 3.54 \(\pm\) 0.02  & 4.0 \(\pm\) 0.5  \\
5 & 60 & 5 & ON & 1310 \(\pm\) 0.18  & 4.28 \(\pm\) 0.018  & 15 \(\pm\) 0.0  & 3.53 \(\pm\) 0.05  & 3.1 \(\pm\) 0.38  \\
6 & 60 & 10 & ON & 1358 \(\pm\) 0.18  & 4.28 \(\pm\) 0.018  & 15 \(\pm\) 0.0  & 3.16 \(\pm\) 0.00  & 3.9 \(\pm\) 0.79  \\
7 & 60 & 5 & OFF & 1173 \(\pm\) 0.18  & 4.25 \(\pm\) 0.004  & 14 \(\pm\) 0.0  & 3.03 \(\pm\) 0.03  & 3.9 \(\pm\) 0.79  \\
8 & 50 & 5 & OFF & 1116 \(\pm\) 0.02  & 4.14 \(\pm\) 0.003  & 14 \(\pm\) 0.0  & 3.53 \(\pm\) 0.00  & 4.1 \(\pm\) 0.82  \\
9 & 40 & 15 & OFF & 1119 \(\pm\) 0.18  & 4.13 \(\pm\) 0.007  & 15 \(\pm\) 0.0  & 3.46 \(\pm\) 0.01  & 4.2 \(\pm\) 0.58  \\
10 & 40 & 5 & OFF & 1080 \(\pm\) 0.18  & 3.88 \(\pm\) 0.000  & 14 \(\pm\) 0.0  & 3.47 \(\pm\) 0.00  & 4.8 \(\pm\) 1.20a  \\
11 & 60 & 15 & ON & 1481 \(\pm\) 0.18  & 4.71 \(\pm\) 0.021  1: footnotemark: [ENDFOOTNOTE] & 15 \(\pm\) 0.0  & 3.16 \(\pm\) 0.00  & \(<\)0.5  \\
12 & 60 & 10 & OFF & 1173 \(\pm\) 0.00  & 4.26 \(\pm\) 0.011  1: footnotemark: [ENDFOOTNOTE] & 15 \(\pm\) 0.0  & 3.16 \(\pm\) 0.06  & 3.9 \(\pm\) 0.70  \\
13 & 60 & 15 & OFF & 1192 \(\pm\) 0.18  &As seen in Table 5, the parameters of temperature, time, and the US on/off position were statistically significant (\(p<0.05\)) and all parameters tended to increase the amount of TPC. The R\({}^{2}\) value was 0.9745 for the TPC responses, which was very close to 1 and showed a strong correlation between the independent parameters and responses. The turbidity value, \({}^{\circ}\)Brix value, and color a* of US treated samples compared to untreated PJ were not significantly affected by all factors (\(p>0.05\)). Optimization tests were performed for yeast and mold count and TPC amount responses in the design. With the effect of the higher temperature and the US power-on position, yeast and mold count reached to an undetectable level.