## 3 Results and Discussion
### Overview of Metabolites Discovered in Filed Soil (Contaminated Site)
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & & & & \\ \hline
**t\({}_{\textbf{g}}\) (min),m/z** & **t\({}_{\textbf{t}}\)stat** & \(p\)-Value** & **Mass Error (ppm)** & **Metabolite** & **Metabolism** \\ \hline
14.51\({}_{\textbf{g}}\)1.0081 & \(-\)2.9347 & 0.01087 & \(-\)60.60257231 & Oxalate & -Glycine, serine and -threonine metabolism \\  & & & & & \\
14.42\({}_{\textbf{g}}\)154.9983 & \(-\)2.2578 & 0.04045 & 37.38135766 & Oxalacetate & -Clitate cycle (TCA cycle) \\  & & & & & -Alanine, aspartate and glutamate metabolism \\ \hline
15.50\({}_{\textbf{g}}\)298.0540 & \(-\)2.3965 & 0.031075 & \(-\)29.87970305 & Paraxoon & Aminbenzoate degradation \\ \hline
13.18\({}_{\textbf{g}}\)326.0701 & \(-\)2.7571 & 0.015427 & \(-\)3.307671162 & Rteterapine & \\
15.04\({}_{\textbf{g}}\)64.2011 & \(-\)2.519 & 0.024542 & 29.40706749 & Streptomycin 6-phosphate & Streptomycin biosynthesis \\ (Stepromyes scale) & & & & \\
14.89\({}_{\textbf{g}}\)281.0509 & \(-\)2.521 & 0.024455 & 9.005321238 & Thymidine(Developyrimidine & Pyrimidine metabolism \\ \hline \multicolumn{5}{c}{**Downregulated Metabolites in Field Soil (top Contaminated Soil)**} \\ \hline
**t\({}_{\textbf{g}}\) (min),m/z** & **t\({}_{\textbf{t}}\)stat** & \(p\)-Value** & **Mass Error (ppm)** & **Metabolite** & **Metabolism (KEGG)** \\ \hline
15.74\({}_{\textbf{g}}\)26.1299 & 19.888 & \(5.65\times 10^{-10}\) & 4.653951133 & Sclagine & \\
15.58\({}_{\textbf{g}}\)242.9242 & 16.033 & \(1.77\times 10^{-9}\) & 14.54408612 & 2-lodophenol & \\ \hline
14.85\({}_{\textbf{g}}\)424.2616 & 15.193 & \(5.68\times 10^{-9}\) & \(-\)0.851091841 & 12.00-dione & \\  & & & & \\ \hline
3.09\({}_{\textbf{g}}\)134.0937 & 14.018 & \(2.18\times 10^{-8}\) & 20.32734589 & Transrypromine & \\ \hline
15.43\({}_{\textbf{g}}\)27.1743 & 13.249 & \(1.25\times 10^{-9}\) & \(-\)5.728148531 & Ethhamutol & \\ \hline
15.09\({}_{\textbf{g}}\)457.3471 & 13.097 & \(2.71\times 10^{-9}\) & \(-\)6.252920748 & Galpha-Hydrocyampestanol & Brassinosteroid biosynthesis \\ \hline
11.18\({}_{\textbf{g}}\)317.2923 & 12.924 & \(5.34\times 10^{-8}\) & \(-\)26.49916761 & 17-Propyl-Salpha-android-2-en-17 beta-ol & \\ \hline
11.11\({}_{\textbf{g}}\)273.2666 & 11.969 & 0.000000119 & \(-\)32.65235568 & Taxa-(45),11(12)-diene,Taxa-4.11-diene & Dieterpoid biosynthesis \\  & & & & & Biosynthesis of terpenoids and steroids \\ \hline
12.62\({}_{\textbf{g}}\)32.2501 & 10.362 & 0.000000468 & 2.422853992 & (+)-Frosopinine & \\ \hline
11.51\({}_{\textbf{g}}\)386.1729 & 10.236 & 0.00000134 & \(-\)0.61017392 & Dioncophyline C & \\ \hline
13.58\({}_{\textbf{g}}\)440.3581 & 9.6318 & 0.00000131 & 16.37290739 & 3 beta,A-Dimethyl-4-aza-Salpha-cholestane & \\ \hline \hline \end{tabular}
\end{table} 
Table 4: _Cont._ \begin{table}
\begin{tabular}{c c c c c c} \hline  & \multicolumn{4}{c}{**Downregulated Metabolites in Field Soil (not Contaminated Soil)**} \\ \hline
**t\({}_{\mbox{th (minil),m/z}}\)** & **t\({}_{\mbox{stat}}\)** & \(p\)-Value & **Mass Error (ppm)** & **Metabolite** & **Metabolism (kEGG)** \\ \hline
11.58,459.489 & 9.3486 & 0.00000079 & 2.2258472 & Henriicontane & \\ \hline
7.19,285.2403 & 7.484 & \(8.64\times 10^{-8}\) & 7.4501372 & 2-Metaphy-62-headcornoic acid & \\ \hline
15.51,346.3066 & 6.6331 & 0.00000096 & \(-\)32.962628 & Phytoecaramide & Sphingolipid metabolism \\ \hline
15.83,099.5309 & 6.4734 & 0.0000143 & 21.6185413 & Zn-Bacteria-chlorophyll a & Porphyrin and chlorophyll metabolism \\
14.87,445.191 & 4.5605 & 0.00014874 & 1.879348973 & Blasticlin S & \\ \hline
122.21,414.2045 & 3.0239 & 0.000218 & \(-\)0.923434973 & Ischemic & \\ \hline
14.63,573.4865 & 2.7964 & 0.01731 & \(-\)0.02778249 & 1-O-(IZ-Tetradecency)-2-(9Z-octadecency)-(s-ng)cycled & \\ \hline
14.88,903.5644 & 2.7956 & 0.017375 & 38.74803849 & Bacteria-phophystims & Porphyrin and chlorophyll metabolism \\ \hline \end{tabular}  
Matabolite were semi-identified by high-resolution UHPLC-Culture-MS: Methods in this table were screened statistically significant variables (VIP > 1) and (p=value < 0.05).  
\end{table} 
Table 4: Cont.