Last but not least, after being crushed and ground with a breaker, glass powder can be turned into granules by passing it through sieves [28]. This granulated glass powder is used for a variety of tasks, including path lines, synthetic resin reinforcement, and surface treatment through blasting [28]. Along with enhancing the mechanical properties of the polymers, adding glass powder as reinforcing filler during the polymer processing also lowers the cost of production [33]. This study's main objective was to introduce glass fillers into a polylactic acid (PLA) matrix material in three different forms: beads, flakes, and powder. PLA is a thermoplastic polyester that may be composted and degraded after use. It is made from renewable resources, such as corn starch, cassava roots, or sugarcane [34]. Due to its biocompatibility and bio-absorbability, polylactic acid has been employed in several biomedical applications, including rapid drug delivery [35; 36]. It is frequently used in the production of food packaging [37], textiles, and medical implants [38]. Although biodegradable plastics, such as PLA, are better for the environment than conventional plastics made of fossil fuels, they do not have the same thermal, mechanical, or rheological characteristics [36]. Due to their limited compatibility with other materials and the current recycling methods, biodegradable plastics frequently need to be blended with additives or co-polymerized in order to get the necessary properties [39]. As expected, PLA is widely used in AM as it is the most popular polymer for MEX 3D printing [40]. Its performance and how it is affected by the 3D printing parameters have been thoroughly investigated [41; 42; 43; 44], along with its quality characteristics [45] and its sustainability in the process [46]. Aiming to expand its performance in MEX 3D printing, nanocomposites have been developed [47; 48; 49; 50; 51]. Further exploiting its biocompatibility, composites for medical and culinary applications with antibacterial performance have also been investigated [52; 53; 54; 55]. Finally, it has been investigated in pure form and a matrix in composites, in hybrid AM (HAM) for expanding the fields of application of MEX 3D printing [56; 57; 58]. For glass-based composites featuring PLA as the matrix material, a novel method for 3D printing composites of continuous glass fibers (CGF)/ PLA was proposed [59]. The results showed that with a fiber content of up to 45 wt.%, the flexural strength and modulus of CGF/PLA composites created through 3D printing were 313 MPa and 21.5 MPa, respectively. The composites' excellent impregnation and a large fraction of glass fibers gave them mechanical properties that were on par with those of composites reinforced with carbon fibers [59]. This research was focused on developing innovative composites for MEX 3D printing by combining PLA with glass flakes, beads, or powder with loadings ranging from 0 wt.% to 9 wt.%, which according to the authors' best knowledge, has never been presented in the literature so far. The aim was to develop composites with biocompatible and eco-friendly ingredients sourced from natural resources, with improved mechanical performance for the MEX 3D printing process. As mentioned above, the PLA matrix material is a biocompatible polymer, sourced from natural resources, the same as the glass-based additives investigated herein [60]. The objective was to achieve a proper degree of filler distribution and dispersion, to investigate how the concentration of glass affected the new composites' ability to respond mechanically, and to obtain knowledge about the material's microstructure and mechanism. Raman and energy dispersive spectroscopy (EDS) were used to determine the compounds' chemical and elemental composition. Thermogravimetric analysis (TGA) was used to assess the produced compounds' thermal stability. Additionally, a thorough analysis was conducted on the impact of glass particles on the mechanical behavior of both the manufactured filaments and the 3D-printed samples.