## 3 Results and Discussion
### Bioactivity of Teadenol A  
[PERSON] _et al._[34] and [PERSON] _et al._[35] reported that Teadenol A stimulates the secretion of adiponectin and inhibits the expression of protein tyrosine phosphatase-1B (PTP1B). Adiponectin, a polypeptide that is highly specific to the adipose tissue, has anti-inflammatory and antiatherogeneic properties and beneficial effects on metabolism [36]. Adiponectin, which reduces the relative risk of type 2 diabetes [37], is inversely correlated to visceral adipose tissue [38]. Through its effect on adiponectin secretion, Teadenol A has anti-obesity properties. PTP1B is a negative regulator of insulin and leptin signal transduction [39] and a novel therapeutic target for type 2 diabetes mellitus, obesity, and insulin resistance [40]. Teadenol A may have positive regulatory effects on insulin and leptin signal transduction by inhibiting the expression of PTP1B. Due to its multiple health benefits, Teadenol \begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{2}{*}{**No.**} & **Isolates (GenBank & Length & \multicolumn{2}{c}{**Results of EzTaxon**} \\ \cline{3-5}  & **Accession No.)** & **(bp)** & **Closest Match (GenBank Accession No.)** & **Similarity (\%)** \\ \hline
8 & 1-2-b-1 (KR149610) & 1376 & _Entrouchter homacheri_ ATCC 49162(T) (AFHR01000079) & 99.345 \\
9 & 2-4-b-4 (KR149622) & 1298 & _Microbacterium sediminis_ YLB-01(T) (HQ219727) & 98.54 \\
10 & 1-4-b-7 (KR149612) & 1322 & _Ochrouchtern pseudafromil_ ADV31(T) (DQ369521) & 99.849 \\
11 & 2-3-b-5 (KR149618) & 1323 & _Ochrouchtern pseudafromil_ ADV31(T) DQ365921 & 99.697 \\
12 & 1-1-b-8 (KR149609) & 1384 & _Pantagonas_ LMG 2603(T) (DQ504305) & 100 \\
13 & 3-3-b-2 (KR149596) & 1373 & _Pseudomonas aeruginosa_ JCM 5962(T) (B) MA01000316) & 99.93 \\
14 & 3-4-b-2 (KR149600) & 1370 & _Pseudomonas aeruginosa_ JCM 5962(T) (B) MA0100316) & 99.93 \\
15 & 3-4-b-1 (KR149599) & 1375 & _Pseudomonas aeruginosa_ LMG 1242(T) (Z76651) & 99.854 \\
16 & 3-5-b-5 (KR149604) & 1363 & _Pseudomonas aeruginosa_ LMG 1242(T) (Z76651) & 99.853 \\
17 & 1-4-b-9 (KR149607) & 1376 & _Pseudomonas aeruginosa_ LMG 1242(T) (Z76651) & 99.854 \\
18 & 1-5-b-2 (KR149613) & 1372 & _Pseudomonas aeruginosa_ LMG 1242(T) (Z76651) & 99.854 \\
19 & 1-4-b-1 (KR149615) & 1371 & _Pseudomonas aeruginosa_ LMG 1242(T) (Z76651) & 99.854 \\
20 & 1-3-b-2 (KR149616) & 1379 & _Pseudomonas aeruginosa_ LMG 1242(T) (Z76651) & 99.855 \\
21 & 2-4-b-1 (KR149619) & 1369 & _Pseudomonas aeruginosa_ LMG 1242(T) (Z76651) & 99.854 \\
22 & 2-4-b-2 (KR149620) & 1330 & _Pseudomonas aeruginosa_ LMG 1242(T) (Z76651) & 99.85 \\
23 & 24-b-5 (KR149623) & 1387 & _Pseudomonas aeruginosa_ LMG 1242(T) (Z76651) & 99.856 \\
24 & 3-3-b-4 (KR149597) & 1377 & _Pseudomonas Berll ATC 1961(T) (AB02406) & 99.564 \\
25 & 3-5-b-2 (KR149602) & 1358 & _Pseudomonas picoglossistic_ FFC951(T) AB009457) & 99.853 \\  
5-f-2 (KR149632) & 510 & _Earlields caltwas_2 (EU661875) & 98.88 \\
48 & 1-5-f-9 (KR149639) & 706 & _Lithithteinis cormpfenfen_9 (JFJ719392) & 100 \\
49 & 2-2-f-3 (KR149629) & 635 & _Rhiapus microspras_1 (AF115729)_ & 100 \\ \hline \hline \end{tabular}
\end{table} 
Table 3: _Cont._A has been used in pharmaceutical drugs, food and feed, cosme, and assay reagents [35]. In this study, the antioxidant properties of Teadenol A were determined _in vitro_. The IC50 values from the DPPH scavenging activity and superoxide anion radical scavenging ability were 64.8 \(\upmu\)g/mL and 3.335 mg/mL, respectively. The IC50 values from total antioxidant capacity and hydrogen donating ability were 17.6 U/mL and 12 U/mL, respectively. Teadenol A may be partly responsible for the health benefits of PFPT. Future studies should assess the exact role of Teadenol A in PFPT and possible synergistic effects with other bioactive compounds. In conclusion, Teadenol A was isolated and identified following SSF of PFPT. The formulation of Teadenol A was dependent on _A. niger_ and _A. tamari_. Teadenol A promoted the secretion of adiponectin, inhibited the secretion of PTP1B, and had antioxidant effects.