## 4 Discussion  
For more than three decades, medicinal mushrooms have been approved in east Asian countries as an adjunct to standard cancer treatments and have an extensive clinical history of safe as a single agent or in combination with chemotherapy [13; 23]. Cultivation methods were refined with time, with respect to the knowledge regarding suitable substrates to grow for certain mushroom species, optimal temperature, initial pH requirement, white light, influence of heavy metal, aseptic techniques, and ways to curb diseases and pests to yield good harvest [17; 24; 25]. Water and ethanol mixture, used for the separation of cordycepin from _Cordyceps militaris_ by a one-step extraction process, could be successfully applied as cost effective with high yield and purity cordycepin production [26]. Methods of near-infrared (NIRS) spectroscopy, compared with the high-performance liquid chromatography, used to predict adenosine and cordycepin concentrations in fruiting bodies of medicinal mushrooms provided excellent accuracy with minimal sample preparation [27]. Comparing the antimicrobial [28], antioxidant, and cytotoxic properties of the methanol extracts from fruiting bodies and fermented mycelia of _C. militaris_, the results revealed the stronger DPPH radical scavenging activity, while the fermented mycelia had stronger total antioxidant capacity, chelating ability, and reducing power, from which it could be suggested that they had their own role and worked in different ways [29]. The bioactive compounds presented in _G. lucidum_ which can have immune system enhancement properties make it very special and classified under Host Defense Potentiators (HDP) [30; 31]. Ganoderma research, specifically regarding its clinical aspects, requires more supportive clarifications for the dosage and side effects in human beings. The mechanism of action of different bioactive molecules isolated from _G. lucidum_ is yet to be elucidated. Figure 2: Polyacrylamide gel electrophoresis of medicinal mushroom samples of _Cordyceps millitaris_ (fresh mycelium (1) and hydrolysate (2), _Ganoderma neo-japonicum_ (fresh mycelium (3)) and _Bacillus subtilis_ NBH MCC 2353 (8) cultured strain sample. Test mixture of proteins of known molecular weight and Bovine serum albumin (66.4 kDa) were used as a standard. A technological model for the creation of a biologically active product based on organic sources has been developed. Extracts obtained as a result of the cultivation of medicinal fungi _Ganoderma lucidum_, _Ganoderma neo-japonicum_, _Cordyceps sinensis_, _Cordyceps militaris_, and the bacterial producer strain _Bacillus subtilis_ were selected as the starting material for the production of the functional product. The results obtained from the _Cordyceps militaris_ extract cultured under industrial conditions by the optimized method demonstrated higher antioxidant activity against the DPPH- radical, which correlated with the results obtained for the total phenolic content. Also, a difference between the fresh mycelium samples versus the other samples of the adult fungal cultures is clearly visible. This is probably due to the fact that at the initial stage of cultivation, the accumulation of substances with antioxidant activity is low, and it is more likely at this stage that enzymes involved in fungal metabolism are present in the mycelial cultures. This highlights the possibility of targeting the cultivation model according to the need to obtain a biologically active extract. In cases where a high presence of phenolic compounds with high antioxidant activity is required, the optimized cultivation method should be applied up to the fruiting body stage, and in cases where the production of a biologically active extract with high enzymatic activity is required, the fresh mycelial culture method should be applied. Many of the enzymes produced by medicinal mushrooms are themselves biologically active substances. This is the case of metalloproteinases, which, in addition to their proteolytic activity, also exhibit fibrinolytic action.