## 3 Results  
### Average Number of Two-Spotted Spider Mite (TSSM) Eggs Per Leaf  
According to the general analysis of the results, the average number of TSSM eggs was significantly influenced by the date of evaluation (F = 30.36; df = 3; \(p<0.05\)), height of sampling (F = 18.44; df = 2; \(p<0.05\)), interaction between height of sampling and date of sampling (F = 6.97, df = 6; \(p<0.05\)), interaction between treatment and date of evaluation (F = 4.73, df = 9, \(p<0.05\)) and interaction between date of evaluation, treatment and height of evaluation (F = 1.66, df = 18, \(p<0.05\)). We confirmed that there was no impact of treatment (F = 1, df = 3, \(p=0.3931\)). According to Figure 1, the average number of TSSM eggs was the lowest on 10 July in the \"Acaricide 1\" (0.5 \(\pm\) 0.28 eggs/leaf) and \"Acaricide 2\" treatments (0.35 \(\pm\) 0.15 eggs/leaf). On 29 July, the highest number of TSSM eggs was recorded for the control treatment (8.1 \(\pm\) 1.82 eggs/leaf). All other values are presented in Figure 1.  
On 10 July, a higher number of eggs/leaf was recorded in the biological treatment group between 0-2 m above the ground (8.53 \(\pm\) 0.81 eggs/leaf). Regarding the second date of evaluation (17 July), the lowest average number of eggs was recorded in the biological control treatment group (at h1--3.4 \(\pm\) 1.14 eggs/leaf; at h2--2.86 \(\pm\) 1.24 eggs/leaf; at h3--3.4 \(\pm\) 0.99 eggs/leaf). On the third date of evaluation, the highest number of eggs per leaf was detected 4--6 m above ground in almost all treatment groups, except for the \"Acaricide 2\" treatment. At the last date of sampling (evaluation), the number of eggs per leaf was the highest at 4--6 m above ground in all treatment groups. All detailed values are presented in Table 4. \begin{table}
\begin{tabular}{c c c} \hline
**Date of Application** & **Treatment (1.350 m2)** & \\ \hline  & **Acaricide 1** & **Acaricide 2** & **Biological Control** \\ \hline
27 June & Nissorun 10 WP (0.14 kg) & / & / \\
3 July & Vertimec Pro (0.17 L) & Vertimec Pro (0.17 L) & / \\  & & & Californicus Breeding System \\
4 July & / & / & (2 boxes or 1.000 bags with \\  & & & 100 mobile stages/bag) \\
17 July & Nissorun 10 WP (0.14 kg) & Nissorun 10 WP (0.14 kg) & / \\ \hline Approximate cost (EUR) * & 48.21 EUR & 34.31 EUR & 610.98 EUR \\ \hline \end{tabular}  
* the prices of synthetic acaricides are calculated according to the price of one litre or kg of preparation per hectare (Nissorun 10 WP = 99.29 EUR/kg; Vertimec Pro = 119.98 EUR/L).  
\end{table} 
Table 3: Comparison of approximate costs of the application of acaricides and biological control agent single release. (For comparison between dates of evaluation within specific treatment groups and sampling heights, lowercase letters; df = 3.119; for control treatment, h1, F = 1.51. \(p\) = 0.2150, h2, F = 2.52, \(p\) = 0.0610, h3, F = 4.97, \(p\) < 0.05; for biological control, h1, F = 1.37, \(p\) = 0.2554, h2, F = 1.71, \(p\) = 0.1693, h3, F = 4.44, \(p\) < 0.05; for Acaricide 1, h1, F = 1.46, \(p\) = 0.2278, h2, F = 9.39, \(p\) < 0.05, h3, F = 5.52, \(p\) < 0.05; for Acaricide 2, h1, F = 2.77, \(p\) < 0.05, h2, F = 2.77, \(p\) < 0.05, h3, F = 10.54, \(p\) < 0.05). (For comparison between treatments on specific evaluation dates at the same sampling height: uppercase letters, df = 3.119; 10 July, h1, F = 3.28, \(p\) < 0.05, h2, F = 3.26, \(p\) < 0.05, h3, F = 5.97, \(p\) < 0.05; for 17 July, h1, F = 7.38, \(p\) < 0.05, h2, F = 10.26, \(p\) < 0.05, h3, F = 16.37, \(p\) < 0.05; for 29 July, h1, F = 1.64, \(p\) = 0.1838, h2, F = 1.53, \(p\) = 0.2107, h3, F = 2.45, \(p\) = 0.0672; for 9 August, h1, F = 1.06, \(p\) = 0.3698, h2, F = 7.36, \(p\) < 0.05, h3, F = 1.84, \(p\) < 0.05).