## 5 Conclusions  
The results of this study provide important insights into shrimp farming in subtropical regions. The addition of FPE to shrimp feeds showed potential to improve growth performance in animals exposed to a suboptimal temperature of 22 \({}^{\circ}\)C. Notably, the inclusion of 1 g kg\({}^{-1}\) FPE promoted faster growth and improved key immunological and microbiological parameters without observed negative effects. Although a higher inclusion level (2 g kg\({}^{-1}\)) did not further improve performance and was associated with an increased abundance of _Vibrio_ spp., no clinical signs of disease or mortality were observed. These findings highlight the importance of appropriate dosing and open avenues for future research on the physiological impacts of chronic cold exposure in _Peneus vunnamei_. While this study does not address all aspects of cold stress in shrimp, it contributes valuable data on growth, immunity, and microbial responses in animals maintained at 22 \({}^{\circ}\)C for 12 weeks. Conceptualization, F.B.H., D.D.S. and F.B.V. ; methodology, F.B.H., D.D.S. and F.B.V. ; validation, F.B.H., C.S.M., M.H.d.A.M. and J.H. ; formal analysis, F.B.H., C.S.M. and S.A.P. ; investigation, F.B.H., C.S.M., M.H.d.A.M. and J.H. ; validation, F.B.H. and S.A.P. ; resources. F.B.V., D.D.S., W.Q.S. and S.M. ; writing--original draft preparation, F.B.H. ; writing--review and editing, F.B.H., F.B.V., S.M. and T.R. ; supervision, F.B.V., D.D.S. and S.M. ; project administration, F.B.V. and D.D.S. ; funding acquisition, F.B.V. and W.Q.S. All authors have read and agreed to the published version of the manuscript. This project received funding from FAPESC (2021 TR100876) and CNPQ (CNPq 403469/2023-6) and [PERSON] and [PERSON] are research fellows from CNPQ (310250/2020-0). * (10) [PERSON]; [PERSON] The Future Is Now: Marine Aquaculture in the Anthropocene. _ICES J. Mar. Sci._**2021**, _78_, 315-322. [CrossRef]
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