## 1 Introduction  
Wounds result from physical, chemical, or mechanical stress, and microbial infections impede the healing process by causing inflammation and tissue damage [1]. Normal skin regeneration is imperative for maintaining hemostasis in healthy individuals and patients with diabetes [2]. Wounds have been treated for decades using topically applied silver sulfadiazine, pentoxiflline, and psoralen, which may harm the skin [3]. These agents have side effects, such as allergic reactions, hyperosmolality, silver staining of the burn wound, and hemolysis [4]; therefore, there is a need to use a new technique that can overcome all these drawbacks. Nanoparticle formulations are promising dosages for wound healing,and silver has been reported to be used for treating pleurodesis and skin cauterization. Nanoparticles are considered safe, less staining, and more potent in treating disease than the previous drugs [5]. Silver nanoparticles (SN) are among the most commonly used engineered nanomaterials with medicinal, industrial, and agricultural applications [6]. They have exceptional physicochemical qualities (high surface to mass) and antibacterial capabilities, giving them an advantage against multidrug-resistant microbes [7]. The SN primarily acts by releasing silver ions (Ag ions), which are reported to exert potent antimicrobial activity [8]. Recently, green synthesized silver nanoparticles (G-SN) can be administered orally, parenterally, or topically for anticancer and wound healing effects [9]. Both _Nitrosalsola (Salsola) vermiculata_ (SV) and Ag ions exhibit antioxidant action, facilitate enzyme detoxification and treat wounds due to their antibacterial activity affecting the fatty acid content of microbial cell walls [10]; therefore, using prepared SN and SV (SN-SV) may improve its stability and solubility and speed wound healing [11].  
The SN and SN-SV are frequently employed in research (notably in biomedical fields) due to their physical and chemical characteristics, such as anticancer, antioxidant, and anti-inflammatory properties [12]. They may act as medication carriers and biosensing materials. Additionally, the SN has therapeutic applications and may be used to treat burns and infections [13;14]. At the same time, SV is considered a reducing agent, effectively synthesizing stable G-SN with minimal cytotoxicity [15]. This green technology approach is environmentally friendly and produces G-SNs of a consistent size [16]. The G-SN approach can inhibit bacterial and fungal growth, including _E. coli_ and _Bacillus subtilis_[17; 18]. The toxicity of chemically synthesized NPs is considered a substantial challenge that would be prevented by the production and use of G-SN [19]. We look for novel ways of synthesizing safe and friendly synthesized G-SN rather than relying on existing techniques [20].  
In the current study, we incorporated the SN-SV in topical creams as a carrier for wound healing. Wound healing may also be sped up when the G-SN is applied topically to the affected areas due to the prevention of oxidative damage [21]. The cream is an oil-in-water (O/W) emulsion that helps the medicaments to penetrate the skin surface layer (stratum corneum) [22]. The present work aimed to eco-friendly synthesize the SN-SV and evaluate their particle size, surface charge, and morphology. As well, the in vivo anti-inflammatory and proliferative properties in an open wound incision can be used to assess the potential role of the SN-SV in hastening skin tissue regeneration in a rat animal model.