## 3 Results and Discussion
### Enzymatic Hydrolysis Effect on Dietary Fiber Composition of SBP and SBP-CO\({}_{2}\)
The polysaccharide hydrolysis using Celluclast\({}^{\circledR}\) 1.5 L had a positive effect on the SDF/IDF ratio, increasing it from 1:12 to 1:10.3 and 1:8.2 in SBP and SBP-CO\({}_{2}\) samples, respectively. The increase in SDF/IDF was reported in other pomace after enzymatic treatment with cellulase [15; 16; 31]. The partial removal of lipophilic compounds through SFE-CO\({}_{2}\) extraction had no notable impact on the efficiency of enzymatic hydrolysis. The observed changes in dietary fiber fractions can be directly related to the site of enzymatic action and the substrate specificity of the enzymes used. The synergistic activity of enzyme complexes containing pectinases, cellulases, and \(\beta\)-glucosidase facilitates the disruption of pectin and lignin networks, allowing better enzymatic access to cellulose-rich regions [32; 33]. In this study, Viscozyme\({}^{\circledR}\) L--a multi-enzyme preparation containing polygalacturonases, cellulase, hemicellulases, xylanas, \(\beta\)-glucanase, and arabinose. Pectinex\({}^{\circledR}\) Ultra Tropical, composed of pectin lysates, polygalacturonases, \(\beta\)-glucanase, and cellulase, showed complementary activity. Comparable reductions in dietary fiber content after Viscozyme\({}^{\circledR}\) L treatment have been reported by [PERSON] et al. [34], while [PERSON] et al. [35] observed increased alcohol-soluble fiber following enzymatic treatment of carrot pomace. In other studies, enzymatic hydrolysis with cellulase increased SDF content [15; 16; 31]; however, the effect depends on factors such as raw material properties, moisture content, and enzyme dosage. [PERSON] et al. [36] observed a decrease in SDF in wheat bran after cellulase treatment, which was associated with the enhanced depolymerization of dietary fiber and increased release of low-molecular-weight carbohydrates at higher enzyme levels. The enzymatic hydrolysis of fiber components such as hemicellulose, cellulose and pectin produce mono- and oligosaccharides, which have an important physiological and technological properties [37]. To further elucidate the results of enzymatic hydrolysis, an analysis of the mono- and oligosaccharideside profiles of SBP was conducted (Figure 1). Enzymatically hydrolyzed SBP was selected for this analysis due to the slightly higher decrease in IDF and IDF content. Figure 1: Effect of enzymatic hydrolysis on the saccharide profile of SBP. Carbohydrate analysis by HPLC showed that the main saccharides in SBP were galacturonic acid (35.9 mg/g d.m. ), DP4 (12.6 mg/g d.m. ), glucose (11.6 mg/g d.m. ), DP3 (10.6 mg/g d.m. ), as well as slightly lower amounts of fructose, galactose, sucrose, oligosaccharides consisting of 5-6 monosaccharide units (8 mg/g d.m.) and sugar alcohols A and B were found. No oligosaccharides consisting of more than 7 monosaccharide units were detected in the control sample, while after hydrolysis with Viscozyme(r) L, Pectine(r) Ultra Tropical and Celluclast(r) 1.5 L they were obtained of 5.6 mg/g d.m., 6.1 mg/g d.m., 10.7 mg/g d.m., respectively. Oligosaccharides consisting of 5-6 monosaccharide units were the most abundant oligosaccharides found in the samples hydrolyzed with Pectine(r) Ultra tropical and Celluclast(r) 1.5 L (47.36% increase compared to control). DP4 and DP3 were the most abundant in the sample hydrolyzed with Viscozyme(r) L (17.64% and 22.05% increase compared to control). [PERSON] et al. [38] investigated the levels of sucrose, glucose and fructose in the black-current pomace, which were lower than those obtained from SBP. [PERSON] et al. [30] investigated the effect of chokeberry pomace hydrolysis with Viscozyme(r) L on changes in glucose and fructose content, reporting an increase of 4-63% in glucose and 35-141% in fructose levels. Hydrolysis of the berry pomace with cellulolytic and xylanolytic enzymes increased the release of carbohydrates from the cell wall.