## 4 Discussion  
There are many ways to treat demineralization. Here, fluoride and tri-calcium phosphate fluoride were added, followed by an LLL. These were evaluated in terms of micro-hardness and mineral content on bovine enamel. Atomic absorption spectroscopy was used to measure calcium, and UV-Vis spectroscopy was used to measure phosphorus. These processes were used to determine mineral loss. Similarly, mineralizing agents were used in the remineralization solution. These methods were used to efficiently identify calcium and phosphorus and indirectly study the mineral components on dental surfaces [16; 18; 19; 20]. The treatments offering the most calcium in the demineralization solution were the control, the tri-calcium phosphate, and the laser-irradiated tri-calcium phosphate; whereas the control, laser, and laser-irradiated tri-calcium phosphate offered the most phosphorus. This suggests that the minerals were not on the dental surface. These groups showed the lowest remineralizing potential. In other studies, tri-calcium phosphate was added to fluoride, and calcium was encapsulated in sodium lauryl sulfate, which was placed on white spot lesions and made dental enamel more resistant [8; 9]. This contradicts the present study. The laser increases hardness and prevents the treated area from becoming a cavitation. Moreover, the effects are higher when fluoride- or calcium-based compounds are irradiated concurrently [11; 12; 13; 14; 17]. Nevertheless, the data show that the mineral content of the laser group was similar to the control. In all examples where fluoride was exposed to laser irradiation, there was no increase in remineralization potential. This puts their effectiveness into question. The group with the highest remineralizing effect was fluoride. Both calcium and phosphorus were found in low levels in the remineralization solution assuming that both components should be precipitated on enamel surfaces and transformed into more resistant tissue. Although tri-calcium phosphate and simple varnish fluoride had 22,600 ppm concentrations, fluoride was the most effective treatment for dental remineralization. This was a model of constant remineralization, and cyclic pH (including periods of demineralization) was used over 15 days to emulate oral conditions in an optimal environment to determine the best performance of each of the mineralizing agents. An additional demineralizing solution was added at a critical pH to create a white spot lesion. The remineralization solution at pH 7.0 was similar to saliva. Figure 2: **(A)** Indentation from Group B: (**B**) Indentation from Group D where the mark is bigger than the previous image, (**C**) Indentation from Group F, showing a softer surface for a greater indentation. \begin{table}
\begin{tabular}{c c c c c} \hline \hline  & **Variable** & **Variable** & **R** & **\(p\)** \\ \hline \multirow{2}{*}{Remineralization solution} & Calcium ions & Microhardness & \(-\)0.268 & 0.0008 \\  & Phosphorus ions & Microhardness & \(-\)0.208 & \(<\)0.002 \\ \hline \hline \end{tabular}  
Negative correlation between the presence of minerals and microhardness (\(p<0.05\)).  
\end{table} 
Table 3: Correlation between decreased mineralization and microhardness value. Despite this model's chemical conditions, there are still biological differences, which might modify results in the laboratory. The enamel surfaces of the samples were polished flat to homogenize them and create the area needed for testing microhardness. This can certainly be a factor because the studied area was susceptible to deep demineralization in accordance with [PERSON] et al. [9]. The Vickers test was used to analyze the surface resistance because it provides indirect information about the mineral content and hardness of dental tissue [2; 15]. The average microhardness of the control and laser groups had a similar remineralizing effect, which contradicts other studies that show that low-power lasers create a harder and more resistant surface than the additional calcium compounds or the control group [12; 14].