## 4 Discussion
[22] studying a _P. cookei_ strain isolated from a Slovakian cave. The pathogenic activity of _P. cookei_ has also been demonstrated by researchers in Australia who isolated this species from skin lesions on the legs [92]. Several species of fungi that degrade keratin were isolated, although they do not belong to the group of dermatophytes. The _Fusarium_ genus was the most frequently isolated among keratinophilic and keratinocyte fungi. The following species were isolated: _Fusarium solani_, _Fusarium chlamdosporum_, and _Fusarium oxysporum_. Although many _Fusarium_ species are classified as soil saprophytes or plant pathogens, some have the ability to infect humans. _Fusarium_ fungi are often considered as an etiological factor causing fungal keratitis [93]; moreover, there are multiple reports of superficial infections such as onychomyces caused by _Fusarium_ spp., mostly by _F. solani_[94]. [PERSON] et al. [95] first described an invasive disease caused by _F. chlamdosporum_. In that study of an immunocompromised patient, lesions caused by _F. chlamdosporum_ were localized in the nasal auric. Many reports have focused on the negative effects of _Fusarium_ on crops. Recently, in the Kashmir division of the northern Himalayan region of India, _F. chlamdosporum_ has been observed as a dangerous pathogen decimating crops. While _F. oxysporum_ and _F. solani_ have often been described as pathogens causing chilli and brinjal diseases, _F. chlamdosporum_ has been described for the first time in this region [96]. Fungi of the genus _Simplicillium_ may perform many different functions in the environment whilst being found in many different ecological niches. Some are presenting saprophytic activity in soil [33] or freshwater [97]. Others can cause plant diseases [98] or maybe entomopathogenic [99]. In this study, there was one species of _Simplicillium_ isolated --_S. aogashimaense_. It was first discovered in the Japanese islands by [PERSON]'s team. Besides _S. aogashimaense_, other _Simplicillium_ species such as _S. subtropicum_, _S. minatense_, _S. cylindproproyrum_, and _S. sympodiphorum_ have been isolated [33]. _Simplicillium aogashimaense_ can be a fungal endophyte of plants, as in _Brachiaria brizantha_[100]. This species can also present mycoparasitic activity. [PERSON] et al. [101] demonstrated the inhibitory effect of this fungus on wheat powdery mildew, whereas it causes some of the highest crop losses in the world. _Tricholadium asperum_ is commonly found in the rhizosphere and can positively affect plant growth [102]. It is known for producing various enzymes, such as cellulases [103], elastase [104], or keratinase, so it has already been isolated from soil by the hair trap method [105]. The keratinocyte fungus _Purpureacillin linacinum_ (_Paicolomyces linacinus_) was isolated from five localizations included in the study. Other results have been obtained by scientists studying soils from Slovakian national parks, where _P. linacinum_ was one of the most frequently isolated fungi with keratinophilic potential [106]. The keratinocyte activity of _P. linacinum_ was reported in a study by [PERSON] et al. [107], where the potential of a keratinase isolated from fungi of Argentine has been proved. In turn, [PERSON] and [PERSON] presented that _Purpureacillin linacinum_ has the ability to grow on animal (feathers) and human (hair, nails) tissues [108]. Two other species, _Metacordyceps chlamdosporia_ and _Marquaudomyces marquaudii_, were isolated from four parks in Wroclaw. Similar results were obtained by [PERSON] et al. [106], where the isolation of _M. chlamdosporia_ was claimed in soil from only one of the trialed parks [106]. _M. chlamdosporia_ was also isolated from a Korean island of volcanic origin by other researchers. This was the first report of _M. chlamdosporia_ in this area [109]. The ability of _M. chlamdosporia_ to utilize keratin from hair was observed by [PERSON] and [PERSON] [110].