## 3 Results and Discussion
### Fatty Acids Profile  
The fatty acids profile of _D. viridis_ TAV01 is presented in Table 3 and was compared with that of other _Dunaliella_ strains. The highest values correspond to palmitic acid (C16:0, 32.1% of total fatty acids (TFA)), followed by \(\alpha\)-linolenic acid (ALA, C18:3n-3, 26.3% of TFA), and linoleic acid (C18:2n-6, 22.8% of TFA). \begin{table}
\begin{tabular}{c c c c c} \hline
**Centents** & _Dunaliella viridis_ **TAV01** & _Dunaliella salina_1** & _Dunaliella salina_2 & _Dunaliella budaruli_3 ** \\ \hline Moisture & 5.01 \(\pm\) 0.11 & n.d. & n.d. & n.d. \\ Ash & 23.7 \(\pm\) 1.0 & 48.7 \(\pm\) 2.5 & 15.89 & 22.8 \(\pm\) 1.2 \\ Lipids & 21.3 \(\pm\) 0.9 & 3.49 \(\pm\) 0.10 & 18.02 & 7.80 \(\pm\) 0.38 \\ Proteins & 35.7 \(\pm\) 1.8 & 10.03 \(\pm\) 0.57 & 25.67 & 21.5 \(\pm\) 0.8 \\ TDF & 9.06 \(\pm\) 0.01 & 8.97 \(\pm\) 0.50 & 2.10 & n.d. \\ \hline \end{tabular} \({}^{1}\)[49], \({}^{2}\)[50], \({}^{3}\)[51].  
\end{table} 
Table 2: Proximate composition (g 100 g\({}^{-1}\) DW) of _Dunaliella viridis_ TAV01. Values are means \(\pm\) SEM. TDF: total dietary fibre. \begin{table}
\begin{tabular}{c c c c c} \hline
**Fatty Acid (\% of TFA)** & _Dunaliella viridis_ & _Dunaliella_ **Dunaliella** & _Dunaliella_ **Dunaliella** **sp.**3  \\  & **TAV01** & _viridis_1  & _Dunaliella_ **sp.**3  \\ \hline C8:0 & Octanoic acid & nd & nd & 0.41 & nd \\ C10:0 & Decanoic acid & nd & nd & 0.13 & nd \\ C12:0 & Lauric acid & nd & 0.21 & nd \\ C14:0 & Myristic acid & \(1.27\pm 0.01\) & \(0.2\pm 0.1\) & nd & nd \\ C15:0 & Pentadecanoic acid & \(1.79\pm 0.07\) & \(0.5\pm 0.1\) & nd & nd \\ C16:0 & Palmitic acid & \(32.1\pm 0.6\) & \(17.9\pm 0.5\) & 14.12 & \(9.19\pm 1.2\) \\ C18:0 & Stearic acid & nd & \(2.2\pm 0.1\) & nd & \(4.27\pm 0.9\) \\ C20:0 & Eicosanoic acid & \(1.34\pm 0.19\) & nd & 1.23 & nd \\ C21:0 & Henetosanoic acid & \(1.77\pm 0.18\) & nd & N.d & nd \\ C22:0 & Arachidic acid & \(0.22\pm 0.01\) & \(0.9\pm 0.1\) & 2.41 & nd \\ C24:0 & Lignoercic acid & \(2.92\pm 0.75\) & nd & nd & nd \\ \hline \(\Sigma\) SFA & & \(42.0\pm 0.3\) & 21.7 & 18.51 & 13.47 \\ \hline C14:1 & Myristoleic acid & nd & \(0.3\pm 0.1\) & nd & nd \\ C15:1 & Pentadecenoic acid & nd & \(1.4\pm 0.1\) & nd & nd \\ C16:1 & Palmiticoleic acid & \(5.66\pm 0.32\) & \(2.5\pm 0.2\) & 2.57 & \(0.80\pm 0.8\) \\ C17:1 & Heptadecenoic acid & & \(3.4\pm 0.1\) & nd & nd \\ C18:1n-9c & Oleic acid & \(2.62\pm 0.76\) & \(8.4\pm 0.1\) & 1.12 & \(22.51\pm 0.7\) \\ C20:1n-9 & cis-11-Eicosenoic acid & nd & nd & nd & \(1.42\pm 0.2\) \\ C22:1 & Enucle acid & nd & nd & nd & nd \\ \hline \(\Sigma\) MUFA & & \(8.28\pm 0.31\) & 16 & 3.69 & 24.74 \\ \hline C16:2 & Hexadecidenciocid & nd & \(0.1\pm 0.1\) & nd & nd \\ C16:4 & Hexadecateraneoic acid & nd & \(10.5\pm 0.5\) & nd & nd \\ C18:3n-6 & \(\gamma\)-Linolenic acid & \(0.16\pm 0.03\) & \(0.8\pm 0.2\) & nd & nd \\ C18:3n-3 & \(\alpha\)-Linolenic acid & \(26.3\pm 0.9\) & \(26.8\pm 0.2\) & 14.79 & nd \\ C18:3 & & & nd & nd & \(44.31\pm 2.1\) \\ C18:2n-6 & Linoleic acid & \(22.8\pm 0.1\) & \(24.0\pm 0.4\) & 2.11 & \(3.84\pm 0.4\) \\ C20:4n-6 & Arachidonic acid & \(0.43\pm 0.26\) & nd & 4.17 & nd \\ C22:6 & cis-4,7,10,13,16,19- & & & & \\  & Docosahexaenoicenoic acid & & & 6.92 & nd \\ \hline \(\Sigma\) PUFA & & \(49.7\pm 0.6\) & 62.2 & 27.99 & 48.15 \\ \hline \(\Sigma\) (n-3) & & \(26.3\pm 0.9\) & & & \\ \(\Sigma\) (n-6) & & \(23.4\pm 0.1\) & & & \\ \(\Sigma\) (n-3)/\(\Sigma\) (n-6) & & \(1.12\pm 8.24\) & & & \\ PUFA/\(\Sigma\) SFA & & \(1.19\pm 0.18\) & & & \\ \hline \end{tabular} \({}^{1}\)[49], \({}^{2}\)[50], \({}^{3}\)[51].  
\end{table} 
Table 3: Fatty acids profile of Dunaliella viridis TAV01. Values are means \(\pm\) SEM shown in percentage of total fatty acids (TFA). The response to salt stress in terms of the fatty acids profile seems to vary among _Dunaliella_ taxa, which can change the contents of unsaturated fatty acids upon a shift to higher NaCl concentration, in particular in salt-tolerant species [18; 22].