# Risk Assessment of Metals in Black Fungus and Culture Substrates Based on Monte Carlo Simulation
## 3 Results
### Risk Assessment of Dietary Exposure to Heavy Metals in Black Fungus
Supplemental Figure S2 shows that after simulation, the maximum value of As in waste bacteria rods is 2.194 mg/kg, the minimum value is 0 mg/kg, and the average value is 0.209 mg/kg. The 90% confidence interval is mainly distributed in the range of 0.009-0.628 mg/kg. The maximum content of Pb was 1.427 mg/kg, the minimum value was 0 mg/kg, and the average value was 0.702 mg/kg. The 90% confidence interval was mainly distributed in the range of 0.156-1.206 mg/kg. The maximum value of Cd content was 1.119 mg/kg, the minimum value was 0.034 mg/kg, and the average value was 0.143 mg/kg. The 90% confidence interval was mainly distributed in the range of 0.039-0.360 mg/kg. The maximum value of Cr was 4.6424 mg/kg, the minimum value was 0.513 mg/kg, and the average value was 1.901 mg/kg. The 90% confidence interval was mainly distributed in the range of 0.618-3.746 mg/kg. And Cr > Pb > As > Cd. \begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline \multirow{2}{*}{\begin{tabular}{c} **Sample** \\ **Class** \\ \end{tabular} } & \multirow{2}{*}{**Element**} & \multirow{2}{*}{**Average Content**} & \multirow{2}{*}{\begin{tabular}{c} **Minimum** \\ **Value** \\ \end{tabular} } & \multirow{2}{*}{\begin{tabular}{c} **Maximum** \\ **Value** \\ \end{tabular} } & \multirow{2}{*}{\begin{tabular}{c} **Median** \\ **mg/kg** \\ \end{tabular} } & \multirow{2}{*}{\begin{tabular}{c} **Limit** \\ **Value** \\ \end{tabular} } & \multirow{2}{*}{\begin{tabular}{c} **Detection** \\ **Ratio** \\ \end{tabular} } & \multirow{2}{*}{\begin{tabular}{c} **Over Standard** \\ **Rate** \\ \end{tabular} } \\  & & & & & & & **mg/kg** \\ \hline \multirow{4}{*}{\begin{tabular}{c} Wheat bran \\ \end{tabular} } & As & 0.118 \(\pm\) 0.173 & 0.016 & 0.425 & 0.059 & 0.8 & 100 & 0 \\  & Pb & 0.632 \(\pm\) 0.613 & 0.008 & 2.221 & 0.608 & 35 & 100 & 0 \\  & Cd & 0.064 \(\pm\) 0.065 & 0.010 & 0.217 & 0.047 & 0.3 & 100 & 0 \\  & Cr & 2.831 \(\pm\) 5.453 & 0.344 & 20.620 & 0.692 & / & 100 & / \\ \hline \multirow{4}{*}{\begin{tabular}{c} Corn meal \\ \end{tabular} } & As & / & / & / & / & 0.8 & 100 & / \\  & Pb & 0.084 \(\pm\) 0.049 & 0.044 & 0.176 & 0.058 & 35 & 100 & 0 \\  & Cd & 0.010 \(\pm\) 0.006 & 0.002 & 0.018 & 0.009 & 0.3 & 100 & 0 \\  & Cr & 0.740 \(\pm\) 0.375 & 0.410 & 1.443 & 0.554 & / & 100 & / \\ \hline \multirow{4}{*}{\begin{tabular}{c} Lime \\ \end{tabular} } & As & 9.810 \(\pm\) 7.615 & 1.941 & 21.215 & 6.891 & / & 100 & / \\  & Pb & 2.606 \(\pm\) 2.547 & 0.088 & 9.344 & 2.014 & / & 100 & / \\  & Cd & 0.151 \(\pm\) 0.228 & 0.001 & 1.056 & 0.072 & / & 100 & / \\  & Cr & 17.709 \(\pm\) 42.477 & 1.574 & 197.720 & 5.659 & / & 100 & / \\ \hline \multirow{4}{*}{\
\begin{tabular}{c} Gypsum \\ \end{tabular} } & As & 1.318 \(\pm\) 1.200 & 0.309 & 3.295 & 1.024 & / & 100 & / \\  & Pb & 1.071 \(\pm\) 1.877 & 0.060 & 4.172 & 0.498 & / & 100 & / \\  & Cd & 0.155 \(\pm\) 0.209 & 0.001 & 0.715 & 0.061 & / & 100 & / \\  & Cr & 2.573 \(\pm\) 1.088 & 1.147 & 5.040 & 2.373 & / & 100 & / \\ \hline \multirow{4}{*}{
\begin{tabular}{c} Irrigation \\ water \\ \end{tabular} } & As & 0.001 & / & / & / & 0.01 & 2.94 & 0 \\  & Pb & No detected & / & / & / & 0.01 & 0 & 0 \\  & Cd & No detected & / & / & / & 0.005 & 0 & 0 \\  & Cr & 0.001 & / & / & / & 0.05 & 2.94 & 0 \\ \hline \hline \end{tabular}
\end{table} 
Table 6: _Cont._Supplemental Figure S3 shows that after simulation, the maximum value of As content in sawdust is 0.323 mg/kg, the minimum value is 0.004 mg/kg, and the average value is 0.035 mg/kg. The 90% confidence interval is mainly distributed in the range of 0.006-0.097 mg/kg. The maximum content of Pb is 1.713 mg/kg, the minimum value is 0 mg/kg, and the average value is 0.804 mg/kg. The 90% confidence interval is mainly distributed in the range of 0.124-1.44 mg/kg. The maximum content of Cd is 1.147 mg/kg and the minimum value is 0.030 mg/kg. The average value was 0.405 mg/kg, and the 90% confidence interval was mainly distributed in the range of 0.059-0.902 mg/kg.