## 2 Materials and Methods  
Cell-mediated immunity (CMI) response is considered to correlate with adherence of T lymphocytes to some solid-state surfaces [39] and interaction forces between human CD4 Ly cells with the antigen [40]. Leukocyte adherence inhibition (LAI) assay is based on microscopic evaluation of in vitro adherence of T lymphocytes to surfaces of specific glass or plastic materials in the presence and in the absence of antigens. Two types of antigens were used. The specific antigen is an immunological functional fraction prepared from a malignant tumor of the same type as the malignancy of the patient from which the blood was taken. The nonspecific antigen is an immunological functional fraction prepared from the serum of inbred laboratory mouse C3H H2k strain infected with the LDH virus. Both antigens prepared by high-pressure gel chromatography are ribonucleic acids (RNA). A comprehensive description of the experimental method including preparation of T lymphocytes and the antigens is available in [9; 33; 34; 37]. Sorption effects for separation of a complex naturally occurring mixture of high-molecular-weight compounds is described in [PERSON] et al. [41] A simple and sensitive test for detection of tumor-specific antigen mediated by macrophage adherence inhibition is in [PERSON] et al. [42]. The gel chromatogram of antigen fractions prepared from human carcinoma and inbred mice (strain C3H) infected with LDH virus is in [PERSON] et al. [37]. Besides chemical reactions, the antigens were separated by a differential refractometer and a spectrophotometric detector, and the data were recorded, using a two-channel Philips PM 8010 recorder [43]. The antigen is a 340 nm fraction. A schematic picture with the receptor, ligand, and signaling pathway is in [PERSON] et al. [36].  
The CMI response by the described method was investigated on patients with cancer of the cervix, endometrium, ovary, breast, and lung, as well as melanoblastoma and otorhinolaryngological cancers. Cancer diagnosis was verified by histopathology, using cell samples taken during surgery. Conditions for malignant transition in cancer development were studied on precancerous cervical lesions. These lesions were diagnosed by cytological (PAP) smears, from examination by colposcopy and from colposcopically directed 'punch' biopsy material by histology. The grades of development of precancerous lesions LSIL and HSIL (low- and high-grade squamous intraepithelial lesions,respectively) were determined by cytology, and CIN 1, 2, and 3 (cervical intraepithelial neoplasia) grades by histology--[PERSON] et al. [37].  
Cells from healthy humans adhere both without and with the antigens, adherence of cancer cells with either antigen is decreased, and the mutual difference is small. The results of LAI assay were evaluated in three regions--healthy, noise, and pathological (e.g., cancer). A relative number is used: \(M=100\cdot m/m_{0}\) (in %) where \(m_{0}\) and \(m\) are numbers of cells in the suspension before and after the sedimentation-adhesion process.  
The LAI assay is a complex and tedious experimental method, and unequivocal results are conditioned by a high purity of antigens. Chemical and physical agents can affect the cell-mediated immunity response [34; 35; 36]. For example, magnetic field 0.5-10 mT decreases (increases) adherence of CD\({}_{4}\)Ly taken from healthy humans (cancer patients). Magnetic field can affect adherence surface of glass, interaction forces between CD\({}_{4}\)Ly, and properties of antigen, but the mechanism of such behavior is unknown.  
A positive response to the nonspecific antigen was also examined in patients with acute myocardial infarction, schizophrenia, and recurrent spontaneous abortions in early pregnancy from unknown reasons [44].