## 2 Materials and Methods
### Mycoremediation Assay  
Untreated soil (control) was compared against four bioaugmentation treatments by adding SMS of _P. eryngii_, _L. edodes_, _P. ostreatus_ and _A. bisporus_. For this, 500 g of polluted soil were mixed with 50 g of each SMS in closed glass containers of 1 L. The control and the treatments were performed in quadruplicate and were kept in the dark for 40 days at 20 \({}^{\circ}\)C and 70% MWHC. During this period, the glass containers were opened once per week to oxygenate them and to add water, when needed to keep the soil moisture. After incubation,sampling, processing and analysis of hydrolase and dehydrogenase activities, ergosterol content, ligninolytic enzymatic activities total TPHs and its aliphatic and aromatic fractions were carried out. To determine the soil microbial activity, two soil enzyme activities were determined, dehydrogenase and total hydrolase activity. Soil dehydrogenase activity determination was performed by reducing the triphenyltetrazolzolium chloride to 1,3,5-triphenylformazan. To this, 1000 g of soil was incubated with 1 mL of 0.1 mM Tris-HCl buffer at pH 7.6 with 1.5% triphenyltetrazolzolium chloride (TCC) for 24 h at 30 \({}^{\circ}\)C. After this time, the reaction was stopped and the 1,3,5-triphenylformazan produced was extracted with acetone, centrifuged and measured in the spectrophotometer at 546 nm (\(\varepsilon\) = 15.4 mM\({}^{-1}\) cm\({}^{-1}\)) [34]. To determine the total hydrolase activity, the fluorescein produced from the hydrolysis of fluorescein diacetate was measured [35]. The soil sample (1 g) was incubated with 60 mM KH\({}_{2}\)PO\({}_{4}\)/K\({}_{2}\)HPO\({}_{4}\) buffer at pH 7.6 and fluorescein diacetate for 20 min at 30 \({}^{\circ}\)C. After that time, acetone was added to stop the reaction and then samples were centrifuged at 5000 rpm for 5 min after a manual stirring. The absorbance of the supernatant was measured at 490 nm in the spectrophotometer (\(\varepsilon\) = 80.3 M\({}^{-1}\) cm\({}^{-1}\)). Ergosterol is a membrane lipid found in fungi that allows for determining the fungal biomass present in the soil [36]. A suspension of 0.5 g of milled soil sample was extracted with 3 mL of KOH 10% _w/v_ in methanol and 1 mL of hexane. This is sonicated for 90 min in a hot water bath at 70 \({}^{\circ}\)C. Subsequently, the extract was filtered, and the soil was washed with hexane. The purification of the extract was carried out by liquid-liquid extraction in a separating funnel. A total of 1 mL of H\({}_{2}\)O was added to the methanol:hexane extract and extracted three times with 2 mL of hexane. The hexane phases were combined and dried over a stream of nitrogen. Subsequently, the solid residue was redissolved in methanol [37]. The extracts were analyzed by high-performance liquid chromatography coupled to a photodiode array detector (HPLC-PDA). Chromatographic separation of ergosterol was achieved with a Phenomenex Luna C18 (2) (150 \(\times\) 4.6 mm; size of 5 \(\upmu\)m particle; 100 A pore size) column using an isocratic elution program with methanol:water (95:5) at a flow rate of 1 mL min\({}^{-1}\). The column temperature was set at 30 \({}^{\circ}\)C. The elution profile was monitored at 282 nm. Peak identification of each sample was based on retention time (15.99 min) and UV spectrum (200-400 nm) of commercially available standard (Thermo Fisher Scientific, Waltham, MA, USA). Before the ligninolytic enzyme determination, an extraction of 3000 g of the soil sample was carried out with 30 mL of extracting solution composed of 2.94 g/L CaCl\({}_{2}\) H\({}_{2}\)O, 4.10 g/L of sodium acetate, 2.86 mL/L of glacial acetic acid and 0.50 mL/L of Tween 80. The mixture was stirred at 4 \({}^{\circ}\)C for 60 min, centrifuged for 15 min at 5000 rpm and finally, the supernatant was collected [7]. To analyze the lactose enzymatic activity, the oxidation of 2,6-dimethoxiphenyl (DMP) was monitored at 477 nm in the spectrophotometer in kinetic mode for 2 min [38].