## 2 Materials and Methods
Before assembling the mixing tips, it was verified that the two components (base and catalyst) would come out uniformly, by exerting a slight pressure on the dispenser lever and extruding a small amount of material, which was removed. Then the mixing tip was inserted into the cartridge, and the material was extruded. Tropicalgin was extracted from the package with the help of the measuring spoon after shaking the bag. For each spoon full of powder placed in the bowl, a 1/3 measure of water was added, then mixed with a plastic spatula until the color and consistency were homogenous. The plug was fully filled with the prepared materials, and a pressure similar to the intraoral conditions was applied on the impression material. After the recommended setting time, the impression materials were checked clinically, to see if they were properly set, as is done in our daily practice. After retrieving the complete set of specimens, they were removed and placed in disinfected trays. \begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Disinfectants** & **Application** & **Active Ingredients/100** & **Action Time** & **Spectrum of Action** \\  & & **g** & **(Minutes)** & **(2)** \\ \hline \multirow{6}{*}{Zeta 3 Soft} & \multirow{6}{*}{Ready-to-use spray} & \multirow{6}{*}{Alcohols (34.4 g ethanol, 14 g isopropanol)} & \multirow{6}{*}{1-5} & \multicolumn{1}{c}{Bactericidal: EN 13727, EN 14561 (_Staphylococcus aureus, Pseudomonas aeruginosa_)} & \multirow{6}{*}{1-5} \\  & & & & & \\ \cline{1-1}  & & & & & \\ \cline{1-1}  & & & & & \\ \cline{1-1}  & & & & & \\ \hline \multirow{6}{*}{Zeta 7 Spray} & \multirow{6}{*}{Ready-to-use spray} & \multirow{6}{*}{Alcohols (83 g ethanol, 10 g 2-propanol)} & \multirow{6}{*}{3} & \multicolumn{1}{c}{Bactericidal: EN 13727} \\  & & & & & (_Staphylococcus aureus, Pseudomonas aeruginosa_)} & \multirow{6}{*}{1-5} \\  & & & & & \\ \cline{1-1}  & & & & & \\ \cline{1-1}  & & & & & \\ \cline{1-1}  & & & & & \\ \hline \multirow{6}{*}{Zeta 7 Solution} & \multirow{6}{*}{Concentrated solution (recommended dilution 1\%)} & \multirow{6}{*}{Quaternary ammonium salts, Phenoxyethanol (7.7 g dimethyl-dideycl ammonium chloride, 15 g phenoxyethanol)} & \multirow{6}{*}{10} & \multicolumn{1}{c}{Bactericidal: EN 13727} \\  & & & & & (_Staphylococcus aureus, Pseudomonas aeruginosa_)} & \multirow{6}{*}{1-5} \\ \cline{1-1}  & & & & & \\ \cline{1-1}  & & & & & \\ \cline{1-1}  & & & & & \\ \hline \hline \end{tabular}
\end{table} 
Table 2: The used-disinfectant properties, according to the manufacturer (Zhernack®). Thirty specimens of each impression material were obtained, totalizing 150 impressions. The specimens were 29 mm in diameter and 15 mm in height. The height of the specimens simulates the maximum height of the impression material in the impression ray at the margins and the edentulous spaces. The specimens from each impression material were divided into three groups according to the disinfectants used, and placed in three separate trays: a. Zeta 3 Soft, b. Zeta 7 Spray, and c. Zeta 7 Solution. Each tray contained ten specimens from each impression material. The following microorganisms were used to contaminate the impressions: _Staphylococcus aureus_ ATCC 25923, _Pseudomonas aeruginosa_ ATCC 27853, and _Candida albicans_ ATCC 10213 (Table 3). All these strains were obtained from the Culture Collection of the Department of Microbiology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Romania. The disk-diffusion method was used to determine the microorganism's susceptibility to the used disinfectants, according to the CLSI (Clinical and Laboratory Standards Institute) and EUCAST (European Committee for Antimicrobial Susceptibility Testing) guidelines and the manufacturer's indications. This qualitative method is among the most flexible susceptibility-testing methods for antimicrobial agents.