###### Abstract  
Abscisic acid (ABA) plays a crucial role in regulating the ripening of non-climacteria strawberry fruit. In the present study, ABA was confirmed to promote strawberry ripening and induce the down-regulation of _FaMADS1_. The transient silence of _FaMADS1_ in strawberries promoted fruit ripening and induced the content of anthocyanin and soluble pectin but reduced firmness and protopectin through a tobacco ratte virus-induced gene silencing technique. In parallel with the accelerated ripening, the genes were significantly induced in the transiently modified fruit, including anthocyanin-related _PAL6_, _CAH_, _4 CL_, _DFR_, and _UFGT_, softening-related _PL_ and _XTH_, and aromat-related _QR_ and _AAT2_. In addition, the interaction between _FaMADS1_ and ABA-related transcription factors was researched. Yeast one-hybrid analysis indicated that the _FaMADS1_ promoter could interact with FaBIB5-5, FaTRAB1, and FaABIB5. Furthermore, dual-luciferase assay suggested that FaTRAB1 could actively bind with the _FaMADS1_ promoter, resulting in the decreased expression of _FaMADS1_. In brief, these results suggest that the ABA-dependent ripening of strawberry fruit was probably inhibited through inhibiting _FaMADS1_ expression by the active binding of transcript FaTRAB1 with the _FaMADS1_ promoter. phyloormone; _Fragaria \(\times\) anannassa_; fruit ripening: MADS; TRAB
receptors in the cytosol [12], and ABAR/CHLH receptors in plastid or chloroplast [13]. In normal conditions, the ABA signal was perceived and transferred by central transduction complex PYR/PP2 Cs/SnRK2s [14]. Activated SnRK2 switched on ABA signaling by phosphorylating the downstream transcription factors such as AREB/ABF, activating several genes sets [15; 16]. Moreover, ABAR could repress the expression of \(W\)_RKY40_ in response to a high level of ABA signal, thereby releasing the expression of the \(ABIS\) gene [17]. A primary region leucine zipper (bZIP) factor, TRAB1, could interact with ABREs and has been identified as a true trans-acting factor involved in ABA-inducible transcription, which controlled maturation and dormancy in plant embryos [18; 19]. However, the complexity and coordination of the network of ABA signaling pathways were more widespread than previously believed. MADS-box genes are present in nearly all major eukaryotic groups and are pivotal regulators in several processes during plant vegetative and reproductive development [20]. Previous studies have reported that MADS-box genes might be the downstream targets of ABA signaling to participate in ABA-associated plant development. In barley, two MADS-box genes, \(H\)_tvOS1_ and \(H\)_tvOS2_, are induced by exogenous ABA, and differences in DNA methylation difference after the application of ABA were suggestive of epigenetic regulation of these genes [20]. MADS-box genes also participated in the ABA-associated flower and fruit development [21; 22; 23]. During the transition from inflorescence to floral merristems, some MADS-box genes have been confirmed to influence the meristem identify genes, regulating the flowering time [24; 25]. A SEP-like gene \(M\)_ADS7_ was cloned in sweet cherry, an indispensable positive regulator of fruit ripening, and exogenous ABA could rescue phenotype defects induced by \(PaMADS7\) silencing [26]. A C-type MADS-box gene \(FaSHP\) was induced by ABA in either over-expression or RNAi-mediated downregulation of strawberry fruit, and over-expression of \(FaSHP\) promoted fruit transformation from green to pink, whereas down-regulation of \(FaSHP\) could delay the aforementioned transformation [6]. Strawberry \(FaMADS1\) belonged to the SEP1/2 clade based on the amino acid sequence and was suppressed by ABA treatment during accelerated ripening [27; 28]. To research the interaction between \(FaMADS1\) and ABA-related transcription factors, strawberry fruit were injected with ABA and a suspension of \(A\). _tumefaciens_ cells carrying a construct for \(FaMADS1\)-RNAi to analyze the function of \(FaMADS1\).