## 3 Results
_tomato_ IZ46, and identified their morphological, cultural, serological properties and ability to induce hypersensitivity reactions on tobacco leaves (HR) (Table 2). According to morphological and cultural properties, induced and spontaneous Str\({}^{\text{R}}\) mutants of _P. syringae_ pv. _tomato_ did not differ from the original strain. In the microagglutination reaction, induced and spontaneous Str\({}^{\text{R}}\) mutants of _P. syringae_ pv. _tomato_ demonstrated a serological relationship with serum to the typical strain of _P. syringae_ pv. _tomato_ R140. All studied Str\({}^{\text{R}}\) mutants of _P. syringae_ pv. _tomato_ induced HR on tobacco leaves that evidenced in favour of the maintenance of virulence that was inherent to the original strains. Previously, during the study of the action of pesticides on the causative agent of wheat basal bacteriosis _P. syringae_ pv. _atrofaciens_, we observed morphological dissociation of phytopathogen with the appearance of R forms in variants with the addition of pesticides [14]. Therefore, we have studied the morphological characteristics of _P. syringae_ pv. _tomato_ at the actions of aluminium phosethyl and deltamethrin. For the strain _P. syringae_ pv. _atrofaciens_ UCM B-1011, the incidence of spontaneous R forms was \(5\times 10^{-3}\)[14]. For the strains of the pathogen of tomato speck _P. syringae_ pv. _tomato_ IZ28 and _P. syringae_ pv. _tomato_ IZ46, the appearance of spontaneous R forms (control) was rare--the frequency of R forms did not exceed \(1\times 10^{-4}\) (Figure 3, Table 3). Under the action of aluminium phosethyl, there was no increase in the frequency of occurrence of the R form of _P. syringae_ pv. _tomato_. At the same time, in terms of the actions of the insecticide deltamethrin, there was observed the appearance of a large number of mutated colonies increased in size--matte, flat with jagged edges (Figure 3), which we have identified as R forms of _P. syringae_ pv. _tomato_. The incidence of R form of _P. syringae_ pv. _tomato_ IZ28 and _P. syringae_ pv. _tomato_ IZ46 in terms of the actions of deltamethrin increased by 100 times in comparison with the frequency of spontaneous \begin{table}
\begin{tabular}{c c c c} \hline \hline \multirow{2}{*}{**Test**} & **Str\({}^{\text{S}}\) (Original Form)** & **Str\({}^{\text{R}}\) Mutants _P. syringae_ pv. _tomato_ IZ28 and IZ46 \\ \cline{2-4}  & _P. syringae_ pv. _tomato_ & **Induced by Aluminium** & **Induced by** \\  & **IZ28 and IZ46** & **Phosethyl** & **Deltamethrin** \\ \hline Gram staining & - & - & - \\ Oxidase & - & - & - \\ Growth on LB Broth & Uniform turbidity & Uniform turbidity & Uniform turbidity \\  & - & - & - \\ glucose, mannose, arabinose, & - & - & - \\ sorbitol, inositol & - & - & - \\ glucose (anaerobic), lactose, maltose & - & - & - \\ HR on tobacco & + & + & + \\  & - & - & \\ Microagglutination reaction with antiserum in dilution: & - & - \\
1:20 & + & + & + \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Physiological and biochemical properties Str\({}^{\text{R}}\) mutants of _P. syringae_ pv. _tomato_. morphological dissociation of these bacteria (Table 3). At the same time, morphological dissociation of the bacteria was observed both at the action of deltamethrin at a concentration of 0.25 mg/L, and at a concentration of 0.025 mg/L of this insecticide. The appearance of R forms is associated with rearrangements in the cell wall elements or polysaccharide capsule, with a change or complete absence of enzymes of biosynthesis of these cell structures as a result of mutation [21; 22]. Such mutations are considered to be pleiotropic, because in addition to changes in the morphology of colonies, there is a decrease in virulence and change of the serological reaction of mutant forms [23]. It was found that according to physiological and biochemical properties, all R dissociants are not different from each other and from the initial S form of _P. syringae_ pv. _tomato_ (Table 3).