## 4 Conclusions  
_Scenedesmus_ sp. and _C. vulgaris_ were used to remove various EDCs. The total removal ratios of parabens (MeP, PrP, and BuP) were higher in the presence of both microalgae, achieving 100% removal within one or two days. Abiotic removal was not significant in this process, with biodegradation being the primary mechanism, while adsorption and bioaccumulation were negligible. BP and E exhibited high abiotic degradation, likely induced by derived ROS. However, the biodegradation pathway contributed significantly to E removal for both _Scenedesmus_ sp. and _C. vulgaris_, achieving 67% and 83% final degradation rates, respectively. Biodegradation was the primary degradation pathway for BPA combined with adsorption. The contribution of the latter pathway was higher in the case of _C. vulgaris_ than in _Scenedesmus_ sp., with final degradation percentages of 90% and 82%, respectively. Overall, the promising results obtained in this work are of interest in the field, as they demonstrate the suitability of _Scenedesmus_ sp. and _C. vulgaris_ for EDC removal. This was proven not only for a specific family of these contaminants but simultaneously for a variety with different characteristics, showing the potential application of these microalgae for EDC removal from wastewater. In addition, the findings reported in this work on the routes implied for EDC removal can be a benchmark for further studies on the metabolic pathways driven by microalgae in these processes. Finally, the results of this research can have future implications for the use of microalgae for wastewater treatment, serving as a starting point to scale up these processes. **Supplementary Materials:** The following supporting information can be downloaded at [[https://www.mdpi.com/article/10.3390/app15010132/s1](https://www.mdpi.com/article/10.3390/app15010132/s1)]([https://www.mdpi.com/article/10.3390/app15010132/s1](https://www.mdpi.com/article/10.3390/app15010132/s1)), Table S1: Analytical parameters obtained for EDCs using HPLC-DAD for the suspended fraction. This includes LOD and LOQ values, calibration curves (where a is the slope and b is the intercept), R\({}^{2}\) values, and the working concentrations ranges; Figure S1: L experiment EDC removal for MeP (black square), PrP (red circle), BuP (blue triangle), BP (green upside down triangle), BPA (purple diamond), and E (orange cross). Error bars represent \(\pm\) standard error of the mean (n = 3); Figure S2: BP removal (a) and E removal (b) for LO experiment (red triangle) and LO without E and BP (blue diamond). Error bars represent \(\pm\) standard error of the mean (n = 3). Figure 7: Contribution (%) of abiotic degradation (yellow), and biodegradation (green _Scenedesmus_ sp. and orange _C. vulgaris_) in E removal by _Scenedesmus_ sp. (**a**) and _C. vulgaris_ (**b**). Error bars represent \(\pm\) standard error of the mean (\(n\) = 3). Contribution of biosorption and bioaccumulation are not plotted because both are negligible. **Author Contributions:** Conceptualisation, J.J.E., G.V. and L.F.B. ; formal analysis, N.G. ; funding acquisition, J.J.E., G.V. and L.F.B. ; investigation, N.G. ; methodology, N.G., R.R., G.V., J.J.E. and L.F.B. ; supervision, J.J.E. and L.F.B. ; writing--original draft preparation, N.G. and R.R. ; writing--review and editing, R.R., G.V., J.J.E. and L.F.B. All authors have read and agreed to the published version of the manuscript. **Funding:** This research was funded by the Ministry of Science and Innovation and co-financed by the European Social and Regional Development Funds (PID2020-114943 RB-I00), the Community of Madrid, and the European Structural Funds (IND2020/AMB-17480) and RENUWAL network (320 RT0005), financed by the CYTED Program. **Institutional Review Board Statement:** Not applicable. **Informed Consent Statement:** Not applicable. **Data Availability Statement:** The original contributions presented in this study are included in the article; further inquiries can be directed to the corresponding author.