###### Abstract  
Bioluminescence imaging has been used to visualize the biological effects of human beings and is a promising technique in a recent modality. In this study, the digital image technique is used to improve quality and recover images. The optical fluence that emerges from the source is generated using a camera, and a low resgolution is observed. In this paper, the diurnal change of ultra-weak photon emission was successfully imaged with an improved, highly sensitive imaging system using a charge-coupled device (CCD) camera. The changes in energy metabolism might be linked with diurnal changes in photon emission, and when observed, the body emits extremely weak light spontaneously without external photoexcitation. Therefore, to obtain accurate information, a combined Barn Door Star Tracker approach has been proposed to improve the accuracy of the method and has been implemented to test on celestial bodies. The ability to temporally assess the location of star movement can be monitored accurately with bioluminescence imaging.  
digital image technology; electronic sensors; low light-emitting sources; photons; Barn Door Star Tracker +
Footnote †: journal: Applied system  
be discussed later on in the paper; hence, we use a process called \"stacking\". Both of these methods, i.e., long exposure and stacking, are tested, and the results are compared. The camera's functioning and the image production are discussed, followed by a proposed design to obtain more efficient/better results. A technology called \"Barn Door Star Tracker\" has been implemented, which does not have any relation with the digital image technology but enormously helps to increase the reach of the work.  
The existence of human bioluminescence [4] was proved by capturing an image showing the glow of five volunteers in the visible light spectrum by a Japanese researcher [PERSON] in the year 2009. This light was bright in the neck and cheek region, emitting a green color. The intensity of the light was only one-thousandth of that which an unaided human eye can perceive; hence, it could not be seen. Due to the lack of technology to capture such a low number of photons, achieving results was extremely difficult, and there was a lag in research. However, over time, digital image technology has advanced rapidly. Now, many middle-class members of society can afford DSLR and mirrorless cameras that truly pack wonders in them in terms of technology and pricing. This has proved to be a motivation to carry out this work and study the possibility of capturing similar images with that which is available. Until now, science has not been able to explain the reason why many mammals possess bioluminescence properly. There have been speculations and suggestions behind this phenomenon, but no concrete correlation has ever been found due to the lack of available data. The proposed work could help in understanding this phenomenon better.  
The goal of this research is to present a convenient setup with an accessible gear, which will allow a larger number of researchers to capture images and collect data on low light-emitting sources such as the human body and faint nebulae. The biggest reason for which we have not been able to unveil the mysteries of human bioluminescence and the science behind it is the lack of data caused by the complex and expensive nature of the experiment. The paper has proven that by merely using the readily available CMOS DSLR/Mirrorless cameras, with the process of stacking and using a Barn Door Tracker, impressive results can be achieved. This will open a window to more research on human bioluminescence (the last research was performed in 2009), and we shall have more data to study and understand the behavior of the human body, thus bringing advances not only to the field of biology, but to essentially make it easier to study any low light-emitting source.