## 3 Results and Discussion
### Antiproliferative Activity of the Water/Methanol Seed Extracts on HT-29 Cancer Cells
Thus, such an extract has been  
\begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline \multirow{2}{*}{**N**} & **R** & **Mass * & \multirow{2}{*}{**Adduct**} & \multirow{2}{*}{**Fragment \({}^{\text{b}}\)**} & **Formula** & **Identification** & **Identification Basis** & **Occurence in Samples \({}^{\text{c}}\)** \\ \hline
11 & 28.81 & 463.08820 & \(\text{[M}-\text{H]}^{-}\) & 302.03696 & C\({}_{21}\)H\({}_{20}\)O\({}_{12}\) & Isoquerctrin & Molecular ion \(\text{[M}-\text{H]}^{-}\)\(m/z\) 463 and \(m/z\) 302, corresponding to the xylose of quercetin & 1B, 2, 4, 5A, 5B, 6, 7, 8 & 1B, 2, 4, 5A, 5B, 6, \\ \hline
12 & 28.83 & 609.14611 & \(\text{[M}-\text{H]}^{-}\) & 301.03474 & C\({}_{25}\)H\({}_{20}\)O\({}_{16}\) & Rutin (quercetin 3-O & Molecular ion \(\text{[M}-\text{H]}^{-}\)\(m/z\) 301 due to the loss of 308 Da (rutinos) & 1A,1B, 2, 3, 4, 5A, 5B, 6, 7, 8, 11 \\ \hline
13 & 29.57 & 287.05501 & \(\text{[M}+\text{H]}^{*}\) & 153.01760 & C\({}_{35}\)H\({}_{20}\)O\({}_{6}\) & Kaemperl & Molecular ion \(\text{[M}-\text{H]}^{-}\)\(m/z\) 287 and \(m/z\) 153, formed by bDA fragmentation wherein bonds 1 and 3 undergo erosion leading to the formation of the A\({}^{\text{a}}\) ion (\(m/z\) 153) [26] & 1A, 1B, 5B, 6, 7, 8, 10 \\ \hline
14 & 29.75 & 447.09328 & \(\text{[M}-\text{H]}^{-}\) & 230.98517 & C\({}_{21}\)H\({}_{20}\)O\({}_{11}\) & Querctrin (quercetin 3-O-Hammingoside) & Molecular ion \(\text{[M}-\text{H]}^{-}\)\(m/z\) 247 and fragment \(m/z\) 231, corresponding to 5B, 6, 7, 8, 9, 10, 11 \\ \hline
15 & 29.77 & 317.03029 & \(\text{[M}-\text{H]}^{-}\) & 151.00262 & C\({}_{35}\)H\({}_{20}\)O\({}_{8}\) & Myricetin & Molecular ion \(\text{[M}-\text{H]}^{-}\)\(m/z\) 317 and a typical & \\  & & & & & MS/MS fragment at \(m/z\) 151, which & 1A,1B, 2, 3, 4, 5A, & corresponded to retrocyclization on the A-C ring & 5B, 6, 7, 8, 10 \\  & & & & & (\({}^{12}\)A\({}^{-}\)) and the consecutive loss of CO & 5B, 6, 7, 8, 10 \\ \hline
16 & 29.80 & 285.04046 & \(\text{[M}-\text{H]}^{-}\) & 121.02799 & C\({}_{35}\)H\({}_{20}\)O\({}_{6}\) & Fisetin & Molecular ion \(\text{[M}-\text{H]}^{-}\)\(m/z\) 285 and \(m/z\) 121, which correspond to fragmentation of the B ring & 5B, 6, 7, 9 \\  & & & & & (\({}^{12}\)B\({}^{-}\)) [28] & & \\ \hline
17 & 29.80 & 285.04046 & \(\text{[M}-\text{H]}^{-}\) & 175.03898 & C\({}_{35}\)H\({}_{20}\)O\({}_{6}\) & Luteolin & Molecular ion \(\text{[M}-\text{H]}^{-}\)\(m/z\) 285 and \(m/z\) 175, corresponding to the loss of C\({}_{3}\)O\({}_{2}\)C\({}_{2}\)H\({}_{20}\) [29] & 1A,1B, 5B, 6, 7, 8, 9 \\ \hline
18 & 29.90 & 447.09328 & \(\text{[M}-\text{H]}^{-}\) & 285.03995 & C\({}_{21}\)H\({}_{20}\)O\({}_{11}\) & Juncein (tueolin-\(\alpha\)-\(\beta\)-glucoside) & Molecular ion \(\text{[M}-\text{H]}^{-}\)\(m/z\) 447 and \(m/z\) 285, the corresponding to luteolin glycone, indicating the loss of a hexose & 1A,1B, 5B, 6, 7, 8, 11 \\ \hline
19 & 29.92 & 447.09328 & \(\text{[M}-\text{H]}^{-}\) & 255.02924 & C\({}_{35}\)H\({}_{20}\)O\({}_{6}\) & Astragalian (kennperl-3-O-G, (kaemperl-3-O, (kaemperl-3-O, (kaemperl-3-O, (kaemperl-3-O, (kaemperl-3-O, (kaemperl-3-O, (kaemperl-3-O, (kaemperl-3-O, (kaemperl-3-O, (kaemperl-3-O, (kaemperl-3-O-, (kaemperl3-O3-,, (kaemperlproposed as a potential candidate for cancer therapy [31]. However, the antitumor activity of phenolic-rich seed extracts of any _Ribes_ species remains unchecked. Figure 2 shows the results of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolzolium bromide (MTT) assay. The concentration-response plots for HT-29 cells after exposure to seed extracts after 48 and 72 h of treatment are drawn in Figures 2A and 2B, respectively, while GI50 for the previous assays and ferulic acid and doxorubicin (positive control) is depicted in Figure 3. The concentration range shown in Figures 2 and 3 (0-300 \(\upmu\)g/mL) was selected because it allows visualizing an asymptotic approximation to a certain formazan absorption level corresponding to maximum decreasing of cell viability.