# Seasonal Variability and Effect of Sample Storage on Volatile Components in _Calypogeia azurea_
## 2 Results and Discussion
### Statistical Analysis of the Obtained Results
#### 2.6.2 Observation of Changes Depending on Storage  
Figure 5 shows the average content of volatile compounds depending on the storage method. Analysis of differences between all individual storage methods, taking into account the duration of use of a given method, showed statistically significant differences only between sesquiterpene compounds, F = 20.58; df = 3; \(p\) < 0.001.  
Pairwise comparisons showed that the severity of the relationship was higher in condition C-5 compared to condition C-2 (\(p\) = 0.005) and C-3 (\(p\) = 0.030), and also lower in condition C-4 compared to condition C-2 (\(p\) = 0.003) and C-3 (\(p\) = 0.017). There were no significant differences between conditions C-2 and C-3, as well as conditions C-4 and C-5. Furthermore, it was confirmed that there were no differences in the storage of volatile compounds such as aliphatics (\(p\) = 0.801), aromatics (\(p\) = 0.266), monoterpenes (\(p\) = 0.072), monoterpends (\(p\) = 0.392), and sesquiterpenoids (\(p\) = 0.122). Additional analysis of the differences between the volatile compounds themselves under particular conditions showedsignificant differences in the case of condition C-2, H = 14, 62; df = 6; \(p\) = 0.023, and for condition C-4, H = 19.08; df = 6; \(p\) = 0.004. As it turned out, volatile sesquiterpene compounds under both conditions showed a higher intensity compared to aliphatic compounds, and these were the only significant differences between all volatile compounds at \(p<0.05\).  
In 2020, they showed statistically significant differences in terms of volatile sesquiterpene compounds, F = 29.03; df = 5; \(p\) < 0.001, and non-identified compounds, F = 11.46; df = 5; \(p\) = 0.043. There were no significant differences between seasons in 2020 for the remaining compounds: aliphatics (\(p\) = 0.207), aromatics (\(p\) = 0.350), monoterenes (\(p\) = 0.221), monoterenoids (\(p\) = 0.416), and sesquiterpenoids (\(p\) = 0.982). Analysis of pairwise comparisons between different substrates in terms of differences between sesquiterpenes and non-identified compounds showed that the level of undefined compound in the C-22 segment was significantly higher than in the C-26 segment (\(p\) = 0.018). However, the intensity  
Figure 4: Average content of volatile organic compounds depending on the season.  
of sesquiterpene compounds in the C-19 segment was significantly higher than in C-18 (\(p\) = 0.013), C-26 (\(p\) < 0.001), and C-27 (\(p\) = 0.002), and, in addition, the compound level in C-26 was lower than in C-23 (\(p\) = 0.035). No significant differences were found in the remaining conditions. In 2021, the comparisons showed statistically significant differences only for volatile sesquiterpene compounds, F = 26.27; df = 5; \(p\) < 0.001. The results of the analyses confirmed that the sesquiterpene compounds had a significantly lower intensity in the C-31 (\(p\) = 0.005) and C-39 (\(p\) < 0.001) segment compared to C-32; moreover, in the C-39 condition, a lower level of sesquiterpene was found compared to C-36 (\(p\) = 0.026). There were also no significant differences in 2021 for the remaining compounds: aliphatics (\(p\) = 0.609), aromatics (\(p\) = 0.152), monoterpenes (\(p\) = 0.371), monoterpencids (\(p\) = 0.416), not identified (\(p\) = 0.118), and sesquiterpenoid (\(p\) = 0.885).