## 4 Conclusions  
The present research contributes toward better chemical characterization of marine green alga _Codium bursa_ ([PERSON]) C. Agardh from the Adriatic Sea. According to the obtained results, the composition of volatile compounds differed significantly considering applied extraction methods. The major headspace compound of HS-F was 2-thiopropane (DMS), while in HS-D it was present as a minor constituent. (_E_)-phytol was obtained in HD-D and in the SC-CO\({}_{2}\) extract as a major constituent, and its predominance was probably originated after chlorophyll breakdown. HD-F was abundant in higher aliphatic hydrocarbons, such as heptadecane and docosane, probably as the consequence of DMS loss. The presence of loliolide was determined only in SC-CO\({}_{2}\) extract. Therefore, the use of different methods enabled obtaining full chemical profiles of the headspace, volatile, and semi-volatile compounds and combined used of all of them can be recommended for chemical profiling of _C. bursa_ (the single-use one method would result with incomplete profiles that are clearly visible even with HS-SPME when two fibers revealed significantly different results). The obtained results for fatty acids of _C. bursa_ showed a predominance of unsaturated fatty acids over the saturated fatty acids. Oleic acid was found as the main fatty acid, followed by palmitic, linoleic, and stearic acid.  
There is a significant difference in the activity against tested fungal species when H\({}_{2}\)O and DMSO extracts were used that could be the influence of different solvent polarity to the compounds' extraction. H\({}_{2}\)O extract exhibited better antifungal effects against _Fusarium_ spp., while DMSO extract was better for the inhibition of _P. expansum, A. flavus_, and _Rhizophus_ spp. On the other hand, the extracts showed growth inducing properties for some fungal species. H\({}_{2}\)O extract showed growth inducing properties when tested on _Rhizopus_ spp. and _P. expansum_, while DMSO extract showed inhibition properties. In general, it can be concluded that the extracts of _C. bursa_ showed relatively good antifungal activity, especially against _P. expansum_.  
This research contributes to a better characterization of marine macroalgae from the Adriatic Sea and provides information about marine algal biodiversity. This alga can be of great interest for future investigations considering its phytochemical profile, as well as biologically active compounds providing observed antifungal activity.  
All the authors discussed and planned the paper; [PERSON]-M.C., [PERSON], [PERSON] and I.J. drafted the manuscript; [PERSON]-[PERSON] performed SC-CO\({}_{2}\) extraction; [PERSON] and [PERSON] preformed HS-SPME, HD, and analysis of VOCs by gas chromatography; [PERSON] performed GC-FID analysis for fatty acids; [PERSON] performed antifungal testing; [PERSON] performed freeze-drying of _C. bursa_; [PERSON] and [PERSON] reviewed and edited the manuscript; I.J. funding acquisition.  
This research was supported by the Croatian Government and the European Union through the European Regional Development Fund-the Competitiveness and Cohesion Operational Programme (KK.01.1.1.01) The Scientific Centre of Excellence for Marine Bioprospecting-BioProCro.  
The authors declare no conflict of interest.