## 3 Results
### Statistical Analysis  
The analysis of MIC values revealed that the combinations of Oregano EO + Vitamin C and Thyme EO + Vitamin C resulted in a statistically significant reduction in MIC values compared to the individual essential oils (\(p<0.05\)). The observed differences in MIC values among the various samples were statistically significant, confirming the synergistic effect of vitamin C when combined with essential oils. Results from the blood agar method further demonstrated significantly lower MBC/MFC values for the combined essential oils compared to the individual oils (\(p<0.05\)). In contrast, differences observed using the agar dilution method were less pronounced, showing a general trend toward improved activity of the combinations, though these differences did not reach statistical significance (\(p>0.05\)).Correlation analysis indicated a statistically significant positive correlation between the concentration of essential oils and their antimicrobial activity. The combinations with vitamin C exhibited an enhanced effect, further reinforcing the synergistic interaction between essential oils and vitamin C in antimicrobial applications. A summary of the results is presented in Figures 1-3.  
\begin{table}
\begin{tabular}{c c c c c} \hline \hline  & \
\begin{tabular}{c} **MBC/MFC** \\ **OEO+ Vit C** \\ **(\(\
icefrac{{p}}{{\text{Value}}}\))** \\ \end{tabular} & \
\begin{tabular}{c} **MBC/MFC** \\ **TEO+ Vit C** \\ **(\(\
icefrac{{p}}{{\text{Value}}}\))** \\ \end{tabular} &
\begin{tabular}{c} **MBC/MFC** \\ **TEO+(\(\
icefrac{{p}}{{\text{Value}}}\))** \\ \end{tabular} \\ \hline \multirow{2}{*}{_E. coli_} & **4\%** & **5\%** & **Lack of** & **5\%** \\  & _(\(p\approx 0.05\))_ & _(\(p\approx 0.05\))_ & **activity** & _(\(p\approx 0.05\))_ \\ \multirow{2}{*}{_S. aureus_} & **5\%** & **5\%** & **4\%** & **5\%** \\  & _(\(p\approx 0.05\))_ & _(\(p\approx 0.05\))_ & _(\(p\approx 0.05\))_ & _(\(p\approx 0.05\))_ \\ \multirow{2}{*}{_C. albicans_} & **2\%** & **2\%** & **5\%** & **5\%** \\  & _(\(p\approx 0.05\))_ & _(\(p\approx 0.05\))_ & _(\(p\approx 0.05\))_ & _(\(p\approx 0.05\))_ \\ \hline \hline \end{tabular}
\end{table}
Table 3: Determination of MBC/MFC of combinations oregano EO–vitamin C and thyme EO–vitamin C against _E. coli_, _S. aureus_ and _C. albicans_ using the agar dilution method.  
Figure 1: MIC (mean \(\pm\) SD) of essential oils and vitamin C against _E. coli, S. aureus_ and _C. albicans_.  
Figure 3: MBC/MFC (mean \(\pm\) SD) of essential oils and vitamin C against _E. coli, S. aureus_ and _C. albicans_ using the agar dilution method.  
Figure 2: MBC/MFC (mean \(\pm\) SD) of essential oils and vitamin C against _E. coli, S. aureus_ and _C. albicans_.  
The experimental results from the combination of oregano and thyme essential oils with vitamin C demonstrated a significant synergistic effect against certain pathogens, while exhibiting an antagonistic effect against others. To complement the observed antimicrobial effects, we performed in silico analyses to identify potential human protein targets and related biological pathways modulated by the active compounds. We analyzed the interactions between the target proteins, mapped their biological function networksand identified the biochemical pathways influenced by these molecules to better understand how these interactions contribute to their antimicrobial effects.