# Application of Mammalian Nudix Enzymes to Capped RNA Analysis
## 5 RNA Decapping with Mammalian Nudt2, Nudt5, Nudt12, Nudt16, and Dcp2
\begin{table}
\begin{tabular}{c c c c} \hline  & **Dcp2** & **Nudt16** & **Nudt12** & **Nudt2** \\ \hline GppppG & & & + [33] \\ GppppA & & & + [33] \\ \hline AppppA & & & + [33] \\ \hline AppppG & & & + [33] \\ \hline m\({}_{2}\)\({}^{7,3}\)\({}^{O}\)GpppG & + [58,89,136] & \(-\)[92,93,120] & \\ \hline m\({}_{2}\)\({}^{7,2}\)\({}^{O}\)GpppG & + [38] & & \\ \hline m\({}_{2}\)\({}^{7,2}\)\({}^{O}\)GppppG & + [136] & & \\ \hline m\({}_{2}\)\({}^{7,2}\)\({}^{O}\)GppCH\({}_{2}\)ppG & \(-\)[136] & & \\ \hline m\({}_{2}\)\({}^{7,2}\)\({}^{O}\)GppCCl\({}_{2}\)ppG & \(-\)[136] & & \\ \hline m\({}_{2}\)\({}^{7,2}\)\({}^{O}\)GppCF\({}_{2}\)ppG & \(-\)[136] & & \\ \hline m\({}_{2}\)\({}^{7,2}\)\({}^{O}\)Gpp-tz-C\({}_{2}\)H\({}_{4}\)OppG & \(-\)[64] & & \\ \hline m\({}_{2}\)\({}^{7,2}\)\({}^{O}\)Gppp-tz-C\({}_{2}\)H\({}_{4}\)OpG & + [64] & & \\ \hline m\({}_{2}\)\({}^{7,2}\)\({}^{O}\)GppNHpN & \(-\)[56] & & \\ \hline m\({}_{2}\)\({}^{7,3}\)\({}^{O}\)GppCH\({}_{2}\)pN & \(-\)[56,58] & & \\ \hline m\({}_{2}\)\({}^{7,2}\)\({}^{O}\)GppPSG (D1) & \(-\)/+ [38] & & \\ \hline m\({}_{2}\)\({}^{7,2}\)\({}^{O}\)GpppsPG (D2) & \(-\)/+ [38] & & \\ \hline m\({}_{2}\)\({}^{7,2}\)\({}^{O}\)GppBHpG (D2) & \(-\)[38] & & \\ \hline bn\({}^{2}\)m\({}^{7}\)GppG & + [92] & \(-\)/+ [92] & \\ \hline bn\({}^{2}\)m\({}^{7}\)GppspG & & \(-\)[93] & \\ \hline bn\({}^{2}\)m\({}^{7,3}\)\({}^{O}\)GpppG & + [89] & & \\ \hline bn\({}^{2}\)m\({}^{7}\)GppppG & + [89] & & \\ \hline bn\({}^{2}\)m\({}^{7}\)GppppG & & + [93] & \\ \hline bn\({}^{2}\)m\({}^{7}\)GppppG & & \(-\)[93] & \\ \hline (4-CCH\({}_{3}\)-bn)\({}^{2}\)m\({}^{7}\)GpppG & + [92] & \(-\)/+ [92] & \\ \hline (4-di(OCH\({}_{3}\)-bn)-tz-CH\({}_{2}\))\({}^{2}\)m\({}^{7}\)GpppG & + [92] & \(-\)[92] & \\ \hline (4-di(OCH\({}_{3}\)-bn)-tz-CH\({}_{2}\))\({}^{2}\)m\({}^{7}\)GpppG & & \(-\)[93] & \\ \hline (4-di(OCH\({}_{3}\)-bn)-tz-(CH\({}_{2}\))\({}^{2}\)m\({}^{7}\)GpppG & & \(+\)/\(-\)[93] & \\ \hline (4-di(OCH\({}_{3}\)-bn)-tz-(CH\({}_{2}\))\({}^{4}\))\({}^{2}\)m\({}^{7}\)GpppG & & \(-\)[93] & \\ \hline (4-di(OCH\({}_{3}\)-bn)-tz-(CH\({}_{2}\))\({}^{2}\)m\({}^{7}\)GpppG & & \(-\)[93] & \\ \hline (4-di(OCH\({}_{3}\)-bn)-tz-(CH\({}_{2}\))\({}^{2}\))\({}^{2}\)m\({}^{7}\)GpppG & & \(-\)[93] & \\ \hline (4-di(OCH\({}_{3}\)-bn)-tz-(CH\({}_{2}\))\({}^{4}\))\({}^{2}\)m\({}^{7}\)GpppG & & \(-\)[93] & \\ \hline (4-bi-isx)\({}^{2}\)m\({}^{7}\)GpppG & + [92] & \(-\)/+ [92] & \\ \hline (4-CH\({}_{3}\)-th)\({}^{2}\)m\({}^{7}\)GpppG & + [92] & \(-\)/+ [92] & \\ \hline \(2\)\({}^{7}\)\({}^{O}\)/\({}^{3}\)\({}^{O}\)-(FAM-L6N)-m\({}^{7}\)GpppG & + [77] & + [77] & \\ \hline m\({}_{3}\)\({}^{2,2}\)\({}^{7}\)GpppG & & + [120,129] & + [130] \\ \hline m\({}_{3}\)\({}^{2,2}\)\({}^{7}\)GpppA & & \(-\)/+ [121] & \\ \hline \end{tabular}
\end{table} 
Table 2: _Cont._Human Nudt5 is the NUDIX hydrolase that has been linked to nucleotide metabolism and cancer [137]. Nudt5 did not show decapping activity towards m7 GpppG- and GpppG-capped RNAs [116]. Recently, for the recombinant hNudt5, it showed decapping activity towards non-canonical UDP-GlcNAc cap on RNA transcripts in vitro [110] (Table 3). Thus, it could be a potential tool to analysis of UDP-glucose-derived cap synthetic analogues for future investigation of this 5\({}^{\prime}\) end RNA modification in mammalian cells [37].