# In Vivo Biosynthesis of Au and Ag NPs Using Two _Medicago sativa_ L. Genotypes
## 3 Results
### Obtaining Plant Suspension from Seedings for Spectrophotometry
\begin{table}
\begin{tabular}{l l l l l} \hline \hline
**Genotype** & **Precursor** & **Wavelength (nm)** & **Concentration (mg/L)** & **Average Absorbance** & **Standard Deviation** \\ \hline ‘_Kometa_’ & None & 450 & Control & 0.330 & - \\ \hline ‘_Kometa_’ & HAuCl4 & 450 & 50 & 0.506\(\dagger\) & \(\pm\)0.125 \\ \hline ‘_Kometa_’ & HAuCl4 & 450 & 100 & 0.336\(\dagger\) & \(\pm\)0.005 \\ \hline ‘_Kometa_’ & HAuCl4 & 450 & 200 & 0.327\(\downarrow\) & \(\pm\)0.002 \\ \hline ‘_Kometa_’ & None & 650 & Control & 0.112 & - \\ \hline ‘_Kometa_’ & HAuCl4 & 650 & 50 & 0.168\(\dagger\) & \(\pm\)0.040 \\ \hline ‘_Kometa_’ & HAuCl4 & 650 & 100 & 0.117\(\dagger\) & \(\pm\)0.004 \\ \hline ‘_Kometa_’ & HAuCl4 & 650 & 200 & 0.111\(\downarrow\) & \(\pm\)0.0002 \\ \hline ‘_Kometa_’ & None & 470 & Control & 0.112 & - \\ \hline ‘_Kometa_’ & AgNO3 & 470 & 250 & 0.089\(\downarrow\) & \(\pm\)0.016 \\ \hline ‘_Kometa_’ & AgNO3 & 470 & 500 & 0.065\(\downarrow\) & \(\pm\)0.187 \\ \hline ‘_Kometa_’ & AgNO3 & 470 & 1000 & 0.146\(\dagger\) & \(\pm\)0.130 \\ \hline ‘_Kometa_’ & None & 350 & Control & 0.112 & - \\ \hline ‘_Kometa_’ & AgNO3 & 350 & 250 & 0.435\(\dagger\) & \(\pm\)0.229 \\ \hline ‘_Kometa_’ & AgNO3 & 350 & 500 & 0.323\(\dagger\) & \(\pm\)0.149 \\ \hline ‘_Kometa_’ & AgNO3 & 350 & 1000 & 0.266\(\dagger\) & \(\pm\)0.109 \\ \hline ‘_la Bella_’ & None & 450 & Control & 0.289 & - \\ \hline ‘_la Bella_’ & HAuCl4 & 450 & 50 & 0.306\(\dagger\) & \(\pm\)0.012 \\ \hline ‘_la Bella_’ & HAuCl4 & 450 & 100 & 0.216 \(\ast\)\(\downarrow\) & \(\pm\)0.052 \\ \hline ‘_la Bella_’ & HAuCl4 & 450 & 200 & 0.194 \(\ast\)\(\downarrow\) & \(\pm\)0.068 \\ \hline ‘_la Bella_’ & None & 650 & Control & 0.099 & - \\ \hline ‘_la Bella_’ & HAuCl4 & 650 & 50 & 0.103\(\dagger\) & \(\pm\)0.003 \\ \hline ‘_la Bella_’ & HAuCl4 & 650 & 100 & 0.099= & \(\pm\)0.017 \\ \hline ‘_la Bella_’ & HAuCl4 & 650 & 200 & 0.069 \(\ast\)\(\downarrow\) & \(\pm\)0.021 \\ \hline ‘_la Bella_’ & None & 470 & Control & 0.269 & - \\ \hline ‘_la Bella_’ & AgNO3 & 470 & 250 & 0.195 \(\ast\)\(\downarrow\) & \(\pm\)0.052 \\ \hline ‘_la Bella_’ & AgNO3 & 470 & 500 & 0.282\(\dagger\) & \(\pm\)0.010 \\ \hline ‘_la Bella_’ & AgNO3 & 470 & 1000 & 0.199 \(\ast\)\(\downarrow\) & \(\pm\)0.049 \\ \hline ‘_la Bella_’ & None & 350 & Control & 0.463 & - \\ \hline ‘_la Bella_’ & AgNO3 & 350 & 250 & 0.354 \(\ast\)\(\downarrow\) & \(\pm\)0.077 \\ \hline ‘_la Bella_’ & AgNO3 & 350 & 500 & 0.512 \(\ast\)\(\uparrow\) & \(\pm\)0.034 \\ \hline \hline \end{tabular}
\end{table} 
Table 5: Light absorbance data at different wavelengths after 24 h of precursor exposure. was significantly increased in all three experimental groups. These results are shown in Table 5.  
The light absorption results of the _Medicago sativa_ L. genotype _'la Bella'_ were also very different. In samples with HAuCl4, illumination at 450 nm showed a statistically significant decrease in absorbance when the precursor was used at concentrations of 100 mg/L and 200 mg/L. However, at a light wavelength of 650 nm, a statistically significant effect on light absorption was seen only when the precursor was used at a high concentration (200 mg/L). Absorption was reduced in _'la Bella'_ samples that received AgNO3 and where absorption was measured at 470 and 350 nm. Statistically significant light absorption decreased when AgNO3 was used at a concentration of 250 mg/L at waves of 470 and 350 nm. However, the absorbance was not significantly affected at 470 nm with a high precursor concentration (500 mg/L), whereas the highest concentration (1000 mg/L) was able to reduce the absorbance statistically significantly. Overall, the results showed that the sample with 500 mg/L AgNO3 at 350 nm had a statistically significant increase, whereas the highest concentration (1000 mg/L) did not significantly affect absorption. Almost all of the light absorption data at different wavelengths showed changed light absorption values after 24 h of exposure to precursors compared to the control group, except for one sample (_'la Bella'_, 650 nm, HAuCl4, 100 mg/L).