## 3 Results
### Gas Chromatography Coupled to Mass Spectrometry (GC-MS)  
In addition to the major constituent (highest percentage), the extract analyses found compounds present in the plant material obtained from all collection areas with percentages above 5% for at least one of them and those were considered to be markers for the parts of the plant. In the extracts of the _D. punctata_ leaves, 24 compounds were identified. The markers were: lup-20(29)-en-3-one, with the highest percentage in area 1 (11%) and \(\gamma\)-sitosterol (5.6%), octadecanoic acid ethyl ester (stearic acid ethyl ester) (7.5%), (-)-spathulenol (9.2%), (Z,Z,Z)-9,12,15-octadecatrienoic acid ethyl ester (linolenic acid ethyl ester) (10.6%), and hexadecanoic acid ethyl ester (palmitic acid ethyl ester) (11.8%) area 2, (Table 4). \begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline  & \multicolumn{3}{c}{**LEAVES EXTRACS**} & **Area 1** & **Area 2** & **Area 3** & **Area 4** & **Area 5** \\ \hline
**N\({}^{*}\)** & **IRT** & **FRT** & **COMPOUNDS** & **RC \%** & **RC \%** & **RC \%** & **RC \%** \\ \hline
01 & 22.47 & 22.48 & 2-Methydroxy-4-vinylphenol & 2.5 & - & 1.7 & - & 3.0 \\
02 & 28.88 & 33.22 & (+)Spathulenol & 4.6 & 9.2 & 3.0 & 3.0 & 2.4 \\
03 & 32.95 & 33.12 & Ethyl 3-(2-hydroxyethyl) propanoate & - & - & - & 1.8 & - & - \\
04 & 39.15 & 39.35 & (E)+(3-Hydroxypropyl-1-en-1-yl)-2-methoxyphenyl & - & - & - & - & 2.1 \\
05 & 42.66 & 42.67 & -Neophytadiene & 2.6 & 4.0 & 3.3 & 4.3 & 3.2 \\
06 & 43.62 & 43.65 & (35,3a6,67,89,84)-1,7-Z trimethyloledhydroxy-3a,7- & 2.1 & - & 1.9 & 2.9 & 2.1 \\
07 & 46.77 & 47.04 & -n-Hexadecanoic acid (palmitic acid) & 4.7 & 4.0 & 5.1 & 4.8 & 3.5 \\
08 & 47.71 & 47.75 & Hexadecanoic acid ethyl ester (Palmitic acid ethyl ester) & 3.8 & 11.8 & 4.6 & 4.6 & 3.6 \\
09 & 49.45 & 52.71 & (Z,Z,9)-12-octadecenoic acid (linolenic acid) & 3.0 & 5.2 & - & - & - \\
10 & 51.29 & 51.38 & & Phytol & 8.0 & 9.8 & 11.1 & 10.2 & 9.0 \\
11 & 52.25 & 52.37 & (Z,Z,9)-12,15-octadecatrienoic acid (linolenic acid) & 3.5 & - & 4.9 & 5.0 & 3.8 \\
12 & 52.72 & 52.74 & (Z,Z,9)-12-octadecatrienoic acid ethyl ester (Linolenic acid ethyl ester) & 2.5 & - & 2.3 & 2.4 & 2.1 \\
13 & 52.89 & 52.95 & (Z,Z,Z,9)-12,15-octadecatrienoic acid ethyl ester (Linolenic acid) & 7.4 & 10.6 & 9.2 & 7.2 & 7.5 \\
14 & 53.71 & 53.75 & Octadecanoic acid ethyl ester (Searatic acid ethyl ester) & 3.2 & 7.5 & 3.3 & 3.3 & 2.7 \\
15 & 54.57 & 54.82 & SaRJ-5,5,8a-trimethyl-2-methylened-xyl-throughhalen-1-yl) & - & - & 2.5 & - & - \\  & & & pent-2-en-1-col (copololol) & & & & & \\
16 & 63.08 & 63.11 & Bis(2-ethyl)/phthalate & 2.2 & - & 2.4 & 2.2 & 1.7 \\
17 & 67.80 & 67.99 & 1,4-Benzencidenary acid acid Bis(2-ethylhexyl) ester & - & - & 1.4 & - & 1.6 \\
18 & 72.36 & 75.75 & 5-Tooperbend & - & - & - & 1.5 & - & 2.0 \\
19 & 78.70 & 78.76 & Stiganset & 3.3 & 4.5 & 3.1 & 3.7 & 3.4 \\
20 & 80.05 & 80.14 & 4, 4, 6a, 6b, 8a, 11, 11, 140-chemethyl,1,4, 4, 4, 5, 6a, 6b, 7, 8, 8a, 9, & 10, 11, 12, 12a,14, 140-octadecatrienoic-2H-picen-3-one & 3.4 & 4.2 & 2.7 & 3.3 & 2.1 \\
22 & 81.27 & 81.42 & Lup-20(29)-3-one & 11.0 & 7.8 & 6.4 & 8.5 & 5.0 \\
23 & 81.87 & 82.15 & Luppel & 24.6 & 15.9 & 22.8 & 27.0 & 34.4 \\
24 & 83.71 & 83.72 & Stiganset-4-en-3-one & 2.6 & - & 2.4 & - \\ Total \% & & & & & & & & \\ \hline \hline \end{tabular} Note: IRT = initial retention time; FRT = final retention time of the compounds obtained in the collection areas; RC % = relative content in percentage; Appendix A (Figures A1-A5) = chromatograms of the leaf extracts in the five collection areas.  
\end{table} 
Table 3: Analysis of phenolic and flavonoid compounds in ethanolic extracts of _Dipteryx punctata_ samples containing representatives from all collection areas. N-hexadecanoic acid (palmitic acid) and phytol were also indicated as markers in _D. punctata_ leaf extracts, with the highest percentages obtained in area 3 (5.1% and 11.1%, respectively) (Table 4). Lupeol predominated in all areas, and in the material collected in areas 4 and 5, the percentages were 27% and 34.4%, respectively (Table 4).