## 3 Results and Discussion  
### HPLC-PDA-tQ-ESI-MS Profiles of SPE Fractions of Silene repens: Qualitative Study  
At the preliminary stage of investigation, solid-phase extraction (SPE) on polyamide was used to separate ecdysteroids (SPE-1) and glycosylflavones (SPE-2) from interfering compounds of total methanol extracts from leaves and roots of introduced samples of _Silene repens_. The chromatographic profiles of SPE-1 and SPE-2 fractions of _S. repens_ total extract were performed by high-performance liquid chromatography with photodiode array detection and electrospray ionization triple quadrupole mass spectrometric detection (HPLC-PDA-ESI-tQ-MS) (Figures 1-3). Such approach coupled with chromatographic data, UV-, and mass-spectrometric identification made it possible to find 18 compounds in SPE extracts of _S. repens_ using the reference standards. Figure 1: High-performance liquid chromatography with photodiode array detection (PDA: 250 nm) of _S. repens_ SPE-1 fractions: leaves, I and roots, II (control 1 sample). Compounds: **1—**26-hydroxyingristperone A; **2—**20, 26-dihydroxyoxyedysone; **3—**26-hydroxypolodine B; **4—**integerrone A; **5—**turkesterone; **6—**20-hydroxyedysone; **7—**polypodine B; **8—**26-hydroxyedysone; **9—**ecdysone; **10—**2-deoxy-20-hydroxyedysone; **11—**20-hydroxyedysone 2-acetate; **12—**vitticosterone E.  
As a result of chromatographic analysis of the SPE-1 fractions from introduced samples of _S. repens_ leaves and roots, the presence of 12 ecdysteroids was revealed (Figure 2). The introduced samples of _S. repens_ did not differ in the ecdysteroid profile from the wild-growing samples [18]. 20-Hydroxyecdysone (**6**; leaves, roots) and polypodine B (**7**; leaves) were the main ecdysteroids of introduced samples in _S. repens_. Integrsterone A (**4**), 20-hydroxyecdysone (**6**), polypodine B (**7**), ecdysone (**9**), and 2-deoxy-20-hydroxyecdysone (**10**) previously were detected in the wild and introduced samples of _S. repens_ from Mongolia [36]. Earlier, 26-hydroxyingristerone A (**1**) and 20,26-dihydroxyecdysone (**2**) were revealed in _S. frivaldszkyana_[37] and 26-hydroxypolypodine B (**3**) was detected in _S. viriidiflora_[38]. Turkesterone (**5**) and viticosterone E (**12**) were found in _S. linicola_[39] and 20-hydroxyecdysone 2-acetate (**11**) was revealed previously in _S. otites_[40]. Chromatographic analysis of the SPE-2 fraction from introduced samples of _S. repens_ leaves revealed the presence of 6 glycosylflavones (**13-18**) in \(O\)-glycoside, \(C\),\(O\)-glycoside,  
Figure 3: High-performance liquid chromatography with photodiode array detection (HPLC-PDA: 330 nm, I) of _S. repens_ SPE-2 fraction from leaves (control 1 sample), absorption spectrum (**II**) and mass spectrum of compound **13** (negative ionization, **III**). Compounds: **13—**schafoside-2′′-O-glucose (sileneside E); **14—**schafoside; **15—**isovitexin-2′′-O-glucose (meloside A); **16—**isovitexin; **17—**swertisin-2′′-O-glucose; **18—**swertisin. Figure 2: Structures of compounds **1**–**18** found in _S. repens_. Abbreviation used: \(\beta\)-D-Glc\(p\)—\(\beta\)-D-Glucopyranoside. and aglycone forms identified as derivatives of schaftooside (schaftooside-2\({}^{\prime\prime}\)-O-glucose, **14**; schaftooside, **15**), isovitexin (isovitexin-2\({}^{\prime\prime}\)-O-glucose, **16**; isovitexin, **17**), and swertizin (swertizin-2\({}^{\prime\prime}\)-O-glucose, **18**; swertizin, **19**) by comparing with reference standards (Figure 3). Schaftooside (**14**), isovitexin-2\({}^{\prime\prime}\)-O-glucose (meloside A, **15**), and isovitexin (**16**) were previously found in _S. repens_[19], while swertisin-2\({}^{\prime\prime}\)-O-glucose (**17**) and swertisin (**18**) were identified in _S. repens_ for the first time. Compound **13** is of interest with a UV spectrum characteristic of flavones. According to mass spectral analysis, this compound was assigned to \(C\),O-glycosflavones.