# Structural Identification and Coagulation Effect of _Flammulina velutipes_ Polysaccharides
## 4 Conclusions  
This study isolated two polysaccharides in _F. velitpes_, CHFVP-1 (24.44 kDa) and CHFVP-2 (1497 kDa), and identified the structural characterization and coagulation activity of them. The CHFVP-1 was constructed with \(\rightarrow\)6)-\(\alpha\)-D-Gal\(p\)-(1\(\rightarrow\) residues as backbone, while CHFVP-2 was constructed with \(\rightarrow\)6)-\(\beta\)-D-Gal\(p\)-(1\(\rightarrow\) residues. Moreover, the branch chain of CHFVP-1 might be inferred as \(\rightarrow\)3,6)-\(\alpha\)-D-Man\(p\)-(1\(\rightarrow\)attached with T-\(\beta\)-D-Glc\(p\) or t-\(\alpha\)-L- Fuc\(p\), and that of CHFVP-2 was T-\(\beta\)-D-Glc\(p\). Based on the coagulation assay results of APTT and TT of CHFVP-1, the polysaccharide exerted promote blood clotting primarily through endogenous coagulation pathways.  
Conceptualization, Z.L. and J.W.; Data curation, J.W.; Formal analysis, Z.L. and C.L.; Funding acquisition, J.W., C.M. and W.K.; Investigation, W.S.; Methodology, K.Z.; Project administration, C.M.; Resources, C.M.; Software, Q.Z.; Supervision, W.K.; Validation, W.S. and C.L.; Visualization, Q.Z. and C.M.; Writing--original draft, Z.L., K.Z. and J.W.; Writing--review and editing, W.K. All authors have read and agreed to the published version of the manuscript.  
This research was funded by National Key R&D Program of China, grant number 2018 YED0400200; Major Public Welfare Projects in Henan Province, grant number 201300110200 and The Key Project in Science and Technology Agency of Henan Province, grant number 212102110019 and 202102110283.  
The study was conducted according to the guidelines of the Declaration of Helsinki, and approved by the Ethics Committee of College of Medical, Henan University (HUSOM-2019-035, 2019).  
Not applicable.  
All the data used are included in this paper.  
\begin{table}
\begin{tabular}{c c c c} \hline
**Group** & **PT(s)** & **APTT(s)** & **TT(s)** \\ \hline Control & 13.77 \(\pm\) 0.30 & 15.37 \(\pm\) 0.15 & 13.97 \(\pm\) 0.23 \\ Yunnan Baiyao & 12.20 \(\pm\) 0.15 *** & 14.47 \(\pm\) 0.35 * & 12.57 \(\pm\) 0.11 *** \\ Breviscapine & 14.47 \(\pm\) 0.55 ** & 16.50 \(\pm\) 0.36 ** & 16.20 \(\pm\) 0.53 *** \\ CHFVP-1 & 13.57 \(\pm\) 0.05 *** & 9.60 \(\pm\) 0.42 *** *** & 13.07 \(\pm\) 0.30 *** \\ CHFVP-2 & 15.77 \(\pm\) 0.32 *** *** & 13.95 \(\pm\) 0.78 *** & 13.17 \(\pm\) 0.49 *** \\ \hline \end{tabular}
\begin{tabular}{c c c} \hline Compared with Control, ** ** \(p\) < 0.001, ** ** \(p\) < 0.01, ** **\(p\) < 0.05. Compared with Yunnan Baiyao, ** ** \(p\) < 0.001, ** **\(p\) < 0.05. Compared with breviscapine, ** **\(p\) < 0.001, ** **\(p\) < 0.01.** \\ \end{tabular}
\end{table}
Table 8: Coagulation effects of CHFVP-1 and CHFVP-2 in vitro.  
**Acknowledgments:** We gratefully acknowledge the valuable cooperation of the members of the National R & D Center for Edible Fungus Processing Technology, Henan University in the experiments.  
**Conflicts of Interest:** The authors declare no conflict of interest.  
**Ethical Conduct of Research:** The study obtained ethical clearance from the Ethics Committee of College of Medical, Henan University (HUSOM-2019-035). The rabbit was treated as the guidelines on the care and use of animals for scientific purposes. All the animal procedures were approved by the Ethical Committee of Henan province, China and conducted in accordance with \"Institute ethical committee guidelines\" for animal experimentation and care.