## 5 Conclusions  
The qualitative composition analysis of extracts of callus, cell suspension, and root cultures showed that ecdysteroids and flavonoids are the most promising BAS, from the point of view of industrial and technological production. These compounds make the greatest contribution to the BAS complex of extracts of callus, cell suspension, and root cultures; their biological activity has been established, and their technological production is cost-effective, since it allows these compounds to be sold on the existing market, thereby reducing economic risk. Many people nowadays prefer natural dietary supplements to synthetic medicines. Therefore, the in vitro biotechnological production of callus, cell suspension, and root cultures, under controlled conditions, represents a cost-effective way for the commercial mass production of phytochemicals. The studied extracts of callus, cell suspension, and root cultures of the medicinal plant _Rlaponticum earthquakes_ are readily available and are considered effective, with fewer side effects compared to modern drugs, in the treatment of various diseases. Substances such as flavonoids, ecdysteroids and anthocyanins found in the in vitro callus, cell suspension, and root cultures of _Rlaponticum earthquakes_ have antioxidant activity, the ability to trap free radicals, and cardioprotective, antidiabetic, anti-inflammatory and anti-allergic effects, while some other phytonoid and ecdysteroid compounds show potential antiviral activity. Recently, the flavonoids and ecdysteroids of _Rlaponticum earthquakes_ were proved to be the most effective anticancer agents, due to apoptosis, which causes cell cycle arrest and the inhibition of key enzymes involved in tumor promotion. The use of flavonoids and ecdysteroids as potential drugs for the prevention of many chronic diseases is a topical trend in pharmacology [36]. In the future, it is planned to study the antimicrobial, antioxidant, and antitumor activity of the BAS of extracts of in vitro callus, cell suspension, and root cultures of the medicinal plant _Rlaponicum carthamoides_ for the production of pharmaceuticals and dietary supplements with antitumor, antimicrobial and antioxidant effects. The following are available online at [[https://www.mdpi.com/2076-3417/11/6/2555/s1](https://www.mdpi.com/2076-3417/11/6/2555/s1)]([https://www.mdpi.com/2076-3417/11/6/2555/s1](https://www.mdpi.com/2076-3417/11/6/2555/s1)), Figure S1: \({}^{1}\)H NMR spectrum of identified compound 2-deoxy-5,20,26-trihydroxyecdyson; Figure S2: \({}^{1}\)H NMR spectrum of identified compound 5,20,26-trihydroxyecdyson 20,22-acetonide/; Figure S3: \({}^{1}\)H NMR spectrum of identified compound 2-deoxy-5,20,26-trihydroxyecdyson 20,22-acetonide; Figure S4: \({}^{1}\)H NMR spectrum of identified compound 20,26-dihydroxyecdyson 20,22-acetonide; Figure S5: \({}^{1}\)H NMR spectrum of identified compound 20,22-acetonide. [PERSON], [PERSON] and [PERSON] conceived and designed the research; [PERSON], L.D. and S.S. analyzed and interpreted the data; [PERSON], E.U. and S.S. contributed reagents, materials, analysis tools or data; [PERSON], [PERSON], [PERSON], [PERSON] and O.B. wrote the paper. All authors have read and agreed to the published version of the manuscript. This research was funded by RUSSIAN SCIENCE FOUNDATION, grant number 18-75-10066. Not applicable. The authors declare no conflict of interest. The Identified Compound No. 1 (1 2-deoxy-5,20,26-trihydroxyecdyson)  
\([a]_{D}^{25}\) +41 (c 0.05 MeOH); UV (MeOH) \(\lambda\)max (log \(\varepsilon\)) 243 nm (4.02) nm; \({}^{1}\)H NMR 1H NMR (500 MHz, Chloroform-d) \(\delta\) 6.02 (\(d\), \(J\) = 1.1 Hz, 1H), 4.81 (s, 1H), 4.74 (\(d\), \(J\) = 12.4 Hz, 1H), 4.59-4.51 (m, 2H), 4.43 (\(d\), \(J\) = 7.9 Hz, 1H), 4.27 (s, 1H), 4.10-3.99 (m, 2H), 3.73-3.66 (m, 1H), 3.58-3.47 (m, 2H), 2.20-20.8 (m, 2H), 2.08-2.01 (m, 2H), 1.92-1.50 (m, 15H), 1.50-1.40 (m, 1H), 1.33 (t, \(J\) = 1.5 Hz, 3H), 1.26 (s, 3H), 1.10 (\(d\), \(J\) = 1.4 Hz, 3H), 0.86 (s, 3H). HRESIMS m=z 497.3108 (calculated for C27H45O8, 497.3102).