###### Abstract  
The main treatments for cancer recorded to date include chemotherapy radiotherapy, and surgery. Although we have achieved great success in treating certain types of tumors, there are still many incurable even with the help of modern treatments. Currently, the principles of magnetic-induction hyperthermia in magnetic nanoparticle hyperthermia are considered an effective treatment for cancer cells. As reported in previous articles, these nanoparticles generate a lot of heat that raises the temperatures of tumors, here treating the cancer cells. The other significant potential of magnetic nanoparticles is the ability to combine heat and drug release for cancer treatment. However, within the biologically safe range of AC magnetic fields, the lack of induction heating power and the high criteria for biocompatibility in superparamagnetic-nanoparticle hyperthermia agents still make up the key challenges for the successful clinical application of magnetic hyperthermia. In this study, two different types of iron oxide nanoparticles (\(\gamma\)-Fe\({}_{2}\)O\({}_{3}\), Fe\({}_{3}\)O\({}_{4}\)) were modified with whey protein isolate (WPI) to form bio-modified superparamagnetic nanoparticles with spherical or diamond-shaped structures and diameters between 20 and 100 nm, which demonstrate excellent stability under different conditions. Adriamycin (ADM) has also been successfully loaded onto these nanoparticles and used in this experiment. In vitro and in vivo experimental studies were performed using these WPI-modified nanoparticles on HepG2 tumor models and mice to assess their bioavailability and biological feasibility. The results prove that these WPI-modified nanoparticles Perform satisfactorily in conjunction with hyperthermia to cure tumors completely.  
bio-modified; cancer treatment; \(\gamma\)-Fe\({}_{2}\)O\({}_{3}\) Fe\({}_{3}\)O\({}_{4}\); induction heating; hyperthermia +
Footnote †: journal: Applied Physics Letters  
**Citation:**  [PERSON]; [PERSON].  
stability of drugs [16; 17; 18; 19; 20]. Fourthly, through the targeting ability of magnetic nanoparticles, different drug delivery routes and targeted drug delivery can be implemented [21; 22; 23; 24].  
This study focuses on developing a new high-biocompatibility superparamagnetic nanoparticle (SNPs), which is expected to be used as a magnetic hyperthermia agent that is not only biologically safe but also boosts high AC induction heating power. The entire development has been a challenge. It was necessary to form superparamagnetic nanoparticles by modifying two well-known magnetic nanoparticles (\(\gamma\)-Fe\({}_{2}\)O\({}_{3}\), Fe\({}_{3}\)O\({}_{4}\)) with whey protein isolate (WPI) and applying them to the experiment. From a biological point of view, whey proteins are essential nutrients in life, and WPI can improve the biocompatibility of nanoparticles, promote degradation and metabolism, as well as facilitate the surface modification, drug attachment, and ligand targeting without impeding the magnetism and energy of nanoparticles, these are the reasons why we chose WPI. Subsequently, the potential, granularity, and morphology of both the developed nanoparticles were characterized by using Fourier transform infrared spectroscopy (FTIR), X-ray diffractometer (XRD), transmission electron microscopy (TEM), and scanning electron microscope (SEM). The stability of AC heating power under different ratios and conditions was repeatedly measured and evaluated. At the same time, in order to prove the drug-carrying ability of these nanoparticles, Adriamycin was successfully loaded into these nanoparticles. Adriamycin as an anti-tumor anthracycline antibiotic can be used in mice in vivo experiments. It is slow metabolism during the treatment in mice, showing a significant therapeutic effect. Finally, in vitro and in vivo tests on HepG2 tumor mouse models were carried out to assess the anti-tumor efficiency of the Adriamycin-loaded and WPI-modified superparamagnetic nanoparticles.