## 2 Results
### Microbial Assay
\begin{table}
\begin{tabular}{c c c c c} \hline \hline
**No.** & **Strains** & **Aerial Parts Extract** & **Root Extract** & **Antibiotics \({}^{*}\)** \\ \hline \multicolumn{5}{c}{Zone inhibition in mm (\% inhibition)} \\ \hline
1. & _M. luteus_ PCM 1944 & 8 \(\pm\) 0.8 (36) & 5 \(\pm\) 0.3 (23) & 22.0 \(\pm\) 0.5 \\
2. & _S. annis_ KW-Sac1 & 4.0 \(\pm\) 1.0 (18) & 3.7 \(\pm\) 1.5 (17) & 22.3 \(\pm\) 0.3 \\
3. & _S. agalactica_ PCM 2683 & 4.0 \(\pm\) 0.0 (13) & 0.0 \(\pm\) 0.0 (0) & 30.0 \(\pm\) 1.0 \\
4. & _S. aureus_ PCM 2054 & 6.0 \(\pm\) 0.0 (21) & 4.0 \(\pm\) 0.0 (14) & 28.7 \(\pm\) 0.6 \\
5. & _S. pseudintermedius_ PCM 2741 & 4.0 \(\pm\) 0.0 (13) & 0.0 \(\pm\) 0.0 (0) & 30.7 \(\pm\) 0.3 \\
6. & _B. subtilis_ PCM 1949 & 3.0 \(\pm\) 0.0 (14) & 0.0 \(\pm\) 0.0 (0) & 42.0 \(\pm\) 0.9 \\
7. & _B. cereus_ PCM 2019 & 0.0 \(\pm\) 0.0 (0) & 0.0 \(\pm\) 0.0 (0) & 39.7 \(\pm\) 0.8 \\
8. & _E. coli_ PCM 2057 & 4.8 \(\pm\) 0.0 (18) & 0.0 \(\pm\) 0.0 (0) & 26.0 \(\pm\) 0.5 \\
9. & _P. aeruginosa_ PCM 2058 & 0.0 \(\pm\) 0.0 (0) & 0.0 \(\pm\) 0.0 (0) & 25.0 \(\pm\) 0.6 \\
10. & _S. marcescens_ PCM 501 & 0.0 \(\pm\) 0.0 (0) & 0.0 \(\pm\) 0.0 (0) & 27.0 \(\pm\) 0.4 \\
11. & _P. carotivora_ IOR 1815 & 11.5 \(\pm\) 0.5 (41) & 7.0 \(\pm\) 1.0 (25) & 28.0 \(\pm\) 1.0 \\
12. & _P. atrosepticum_ IOR 1825 & 10.0 \(\pm\) 0.0 (40) & 8.0 \(\pm\) 1.0 (32) & 25.4 \(\pm\) 0.5 \\
13. & _C. albicans_ ATCC 10231 & 0.0 \(\pm\) 0.0 (0) & 0.0 \(\pm\) 0.0 (0) & 35.4 \(\pm\) 0.7 \\
14. & _C. parapsilosis_ KW-Cp1 & 0.0 \(\pm\) 0.0 (0) & 0.0 \(\pm\) 0.0 (0) & 33.3 \(\pm\) 0.5 \\ \hline \end{tabular} \({}^{*}\) reference control—tetracycline for bacteria and amphotericin B for _Candidida_ (30 ug/disc). \(\pm\)SD (standard deviation). ( ) the percent inhibition of microorganisms compared to suitable reference control is in brackets (control = 100%).  
\end{table} 
Table 3: Antimicrobial activity of aerial parts and root extracts of _S. nigrum_ using disc diffusion method (zone of inhibition in mm). or MBC = 500 \(\upmu\)g/mL, respectively). The extracts from the aerial parts also inhibited the growth of endospore-forming bacilli and _E. coli_ but at a concentration of MIC = 500 \(\upmu\)g/mL. The extract from roots did not exhibit activity against _S. aureus_, _Bacillus_ spp., _E. coli_, _P. aeruginosa_ and _C. albicans_ at the tested concentrations. Tables 5 and 6 show antifungal activity of the tested extracts of _S. nigrum_ L. Most of the tested filamentous fungi did not react uniformly to the presence of black nightshade extracts. _Apergillus niger_, _A. versicolor_, _Epiccum n rigrum_ and _Fusarium oxysporum_, depending on the dose and the extract, showed a slight acceleration or slowing down of the colony growth rate (Table 4). The species most stimulated by the extract from the aerial parts of black nightshade at a dose of 500 \(\upmu\)g/mL was _A. flavus_. The coefficient of colonies' growth inhibition for this combination was \(-\)29 mm (growth stimulation). Root extract in the same dose did not strongly stimulate growth (H = \(-\)9 mm), and in lower doses, it inhibited its growth. _Penicillium notatum_ was significantly inhibited by black nightshade root extract. Depending on the dosage, the growth inhibition coefficient ranged from 17-36% (Table 6). The same coefficient with the use of aerial parts extract did not exceed 9%. _Bipolaris sorkoiniana_, after applying black nightshade root extract at a dose of 500 \(\upmu\)g/mL, was inhibited at the level of 43%. The same dose of the extracts from the aerial parts