###### Abstract  
Background: Recent genome-wide association studies demonstrated the association between the prevalence of chronic kidney disease (CKD) and rs11959928, rs626277, and rs7805747 polymorphisms. Materials and Methods: In this study, we investigated the association between CKD and these polymorphisms in patients and controls according to gender. High-resolution melting analysis was performed to detect DAB2 rs11959928, DACHI rs626277, and PRKAG2 rs7805747 single nucleotide polymorphisms. Genomic DNA was extracted from the buffy coat of 163 patients with chronic renal disease and 218 control individuals. Ten percent of the results were also randomly confirmed by direct DNA sequencing. Results: Multivariable logistic regression analysis with adjustment for confounders showed rs7805747 (dominant model) has a statistically significant protective effect in females, and rs11959928 (additive and dominant models) was significantly associated with the prevalence of CKD in males. rs7805747 (recessive model) was significantly associated with the prevalence of CKD in males. Conclusion: The very same genetic variants have different effects in males and females separately. Our results warrant the need for similar studies in larger cohorts.  
chronic kidney disease; genetic polymorphism; high-resolution melting analysis; gender +
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renal disease, while women have a lower risk of CKD progression and mortality [4]. Polymorphisms associated with gender and CKD have been determined [5].  
DAB2 (Dab, mitogen-responsive phosphoprotein, homolog 2) encodes a protein as a tumor suppressor. Genes believed to be causal were found to have a higher expression in the proximal tubule and play a role in endolysosomal function. DAB2, an adaptor protein in the Transforming Growth Factor-\(\beta\) pathway, was identified as a key central node in that region [6]. Chromatin-associated protein encodes by DACH1 (dachshund homolog 1) regulates gene expression. DACH1 is abundantly expressed in podocytes, and its function in dysfunctional podocytes has previously been discussed [7]. It is well known as a tumor suppressor protein whose decreased expression closely correlates with a poor prognosis in a number of malignancies [8]. DACH1 protein regulates the expression of the target gene either by binding to chromatin or by combining with other transcription factors [9; 10]. DACH1 DNA-specific binding results in transcriptional repression of the target gene [11]. Lower DACH1 expression was linked to a GWAS risk variant in human renal tubules [12]. PRKAG2 (protein kinase, AMP-activated, gamma 2 non-catalytic subunit) encodes for AMP-activated protein kinase, which regulates cellular energy status and functions. Computational methods demonstrated that PRKAG2 rs7805747 is associated with CKD and serum creatinine. Its expression was found to be different in CKD cases compared with the control group [13].  
High-resolution melting (HRM) analysis is shown to be a sensitive and cost-effective diagnostic method. The closed-tube system reduces the risk of contamination. We developed a simple and cost-effective triplex HRM assay to scan for variations in the aforementioned genes. In this study, we investigated the single nucleotide polymorphisms (SNPs), which were found to be associated with the estimated glomerular filtration rate (eGFR) among men and women.