## 3 Results
### Compatibility Tests and Formulation of Bacterial Consortia
\begin{table}
\begin{tabular}{l c c c c c c c c c c} \hline \hline
**Strains** & **TmR1b** & **TmR5a** & **GcR15a** & **NUR4a** & **TmR7** & **CcR1d** & **BmP28** & **BmP22c** & **TmP30** & **GpP26d** \\ \hline TmR1b & & + & + & \(-\) & \(-\) & \(-\) & \(-\) & \(+\) & \(-\) & \(-\) \\ TmR5a & + & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(+\) & \(-\) & \(-\) \\ GcR15a & + & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) \\ NUR4a & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(+\) & \(-\) & \(-\) & \(-\) & \(-\) \\ TmR7 & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(+\) & \(+\) & \(-\) & \(+\) \\ CcR1d & \(-\) & \(-\) & \(-\) & \(+\) & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) \\ BmP28 & \(-\) & \(-\) & \(-\) & \(-\) & \(+\) & \(-\) & \(-\) & \(-\) & \(+\) & \(+\) & \(+\) \\ BmP22c & + & \(+\) & \(-\) & \(-\) & \(+\) & \(-\) & \(-\) & \(+\) & \(-\) & \(+\) & \(-\) \\ TmP30 & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(+\) & \(+\) & \(+\) & \(-\) \\ CpP26d & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(-\) & \(+\) & \(-\) & \(-\) & \(-\) & \(-\) \\ \hline \hline \end{tabular}  
* \(-\), not compatible (antimicrobial activity); +, compatible (without antimicrobial activity).  
\end{table} 
Table 5: Compatibility between selected bacterial strains. * (39)_Brachibacterium paraconglomeratum_ LMG 19861T (AJ415377)
* (40)_Brachibacterium ulimentarium_ CNRZ 925T(X91031)
* (41)_Brachibacterium tyroferuntans_ CNRZ 926T (X91657)
* (42)_Brachibacterium sp_. **TmP30 (MW548378)**
* (43)_Brachibacterium urticum_ Lact5.2 (AFC13397)
* (44)_Arthrobacter citreus_ DSM 20133T (X80737)
* (45)_Arthrobacter sp_. **BmP28 (MW548382)**
* (46)_Arthrobacter pokakali_ P3B162T (KM507333)
* (47)_Arthrobacter pitycampac_ DSM 21719T (EU855749)
* (48)_Arthrobacter agilis_ DSM 20550T (X80748)
* (49)_Frondihabitans claudophilus_ CafT13T (FN666417)
* (50)_Frondihabitans pencodani_ RS-15T (FM998017)
* (51)_Frondihabitans austricians_ E1 HC-02T (DQ525859)
* (52)_Frondihabitans sucicola_ GR542T (JX876867)
* (53)_Frondihabitans_**sp. GPP26d (MW548395)**
* (54)_Crutobacterium comassilment_ ATCC 3131T (EF92577)
* (55)_Crutobacterium circum DSM_ 20528T (X77436)
* (56)_Crutobacterium flacacuntzies_ LMG3645T (AJ312209)
* (57)_Crutobacterium pusillum_ DSM 20527T (AJ784400)
* (58)_Crutobacterium sp_. **BmP22c (MW548393)**
* (59)_Pseudomonas syringae_ pv. _tomato_ DC3000T (NR_074597)
* (60)_Pseudomonas wellanae_ PD 2378T (HF558391)
* (61)_Pseudomonas_**sp. TnnR7 (MW548359)**
* (62)_Pseudomonas protegens_ CHA0T (NR_114749)
* (63)_Pseudomonas arsenicxydans_ VC-1T (FN645213)
* (64)_Pseudomonas_**sp. NUR4a (MW548348)**
* (65)_Pseudomonas_ _betica_ a390T (NR_116899)
* (66)_Pseudomonas_ _yananorum_ SH1T (EU557337)
* (67)_Pseudomonas_**sp. CcR1d (MW548345)**
* (68)_Pseudomonas_ _brenyi_ CIP 10664 (AF268968)
* (69)_Pseudomonas_**sp. GcR15a (MW548343)**
* (70)_Pseudomonas_**sp. TnnR5a (MW548356)**
* (71)_Pseudomonas_**sp. _adrina DSM_ 17516T (AF064461)
* (72)_Pseudomonas_ _fluroscens_ CCM 2115T (DQ207731)
* (73)_Pseudomonas azotofurans_ IAM 1603T (D84009)
* (74)_Pseudomonas marginalis_ ATCC 10844T (AB021401)
* (75)_Pseudomonas lactis_ WS 4992T (KP756923)
* (76)_Pseudomonas_**sp. TnnR1b (MW548351)**
* (77)_Halohasta litchfeldiae_ tADL\({}^{\rm T}\)(JF421969)  
Figure 4: Phylogenetic tree of the 10 selected psychrotolerant strains for the formation of potential bacterial consortia. The dendrogram was constructed using the Neighbor-Joining method based on the partial sequence of the 16S rRNA gene. The tree has arbitrarily been rooted by the Antarctic archaeon _Halohasta litchfeldiae_. Values of 1000 bootstraps are informed at the branching point. GenBank accession numbers of 16S rRNA sequences are indicated in parentheses. Scale bar represents 0.05 substitutions per nucleotide positions. The circles represent the regions of Chile. Red circles, Valparaiso Region; Green circles, Libertador General Bernardo O’Higgins Region; blue circles, Nuble Region; and purple circle, Magallanes and Chilean Antarctica Region.