###### Abstract  
Paying attention to the ecosystem service value (ESV) of different karst landform types is of great benefit to the green, sustainable, and high-quality development of the ecological environment in Guizhou Province. Based on the eight-period China Land Cover Dataset (CLCD) from 1985 to 2020, we compared and analyzed the ecosystem service value and its temporal and spatial variation characteristics of different karst landforms with the equivalent factor method in the study. The results revealed that the overall ecological environment of Guizhou Province showed a tendency to improve. Over the past 35 years, the net increase in the ecosystem service value in Guizhou Province was USD 385 million, with the largest increase occurring from 2015 to 2020. The overall spatial distribution is characterized by continuous low values in the middle and concentrated high values in the surrounding areas. Some of the local ecological environment in Guizhou Province is still not well-preserved. The ecosystem service value in the pure-karst area has decreased by USD 122 million over the past 35 years, mainly in Dushan County and Libo County, Qiannan Prefecture. The semi-karst area has increased by USD 367 million, concentrated in the northern and central areas of Guizhou Province. The non-karst area increased by USD 140 million, mostly distributed in Pu'an County, Xingyi City, and Yanhe County. In terms of the ecosystem service value per unit area, the results were as follows: non-karst areas > pure-karst areas > semi-karst areas. The ecosystem service value of each geomorphological area varied with the elevation and slope, showing an inverted \"V\" trend, first increasing and then decreasing. The maximum ecosystem service value in the pure-karst and semi-karst areas was between 800 m and 1100 m above sea level, and the non-karst area was in the range of 500 m to 800 m. The maximum ecosystem service value in the non-karst areas and semi-karst areas was within the gradient of 15\({}^{\circ}\) to 25\({}^{\circ}\), and the pure-karst area was between 6\({}^{\circ}\) and 15\({}^{\circ}\). The forest contributed most to the ecosystem service value of each karst landform, followed by cropland, and finally shrubland and grassland. Guizhou Province should pay attention to the protection of forest and cropland ecosystems in terms of future land management, especially with regard to ecological construction in pure-karst landform areas. p 2022 202029 November 202213 December 2022  
The value of ecosystem services can measure the potential contribution capacity of regional ecosystem services, usually represented in the form of money, which can incorporate environmental issues into socio-economic development decisions, and effectively assist regional spatial planning, ecological regulation, and ecological restoration [4; 5; 6]. The assessment principles and methods of ESV were proposed and practiced by [PERSON] et al. [7; 8]. The Millennium Ecosystem Assessment (MA) project further promoted the research process [9], which also formulated a reference standard of ecosystem services quantification [10], making ESV a hot topic of discussion in the fields of ecology, economics, and geography [11; 12; 13]. Scholars believe that the history of ESV can be divided into four stages: qualitative description (before 1997), rapid development (1997-2005), diversified development (2005-2012), and comprehensive application (2012-present) [14]. The ideas and methods of research on ESV are constantly being innovated. In terms of research areas, the dynamic monitoring of the ESV from land such as watersheds and nature reserves to coastal and marine areas has been realized [15; 16; 17; 18]. In terms of assessment objects, temporal and spatial pattern characteristics and influencing factors of ESV have been chiefly analyzed in croplands, woodlands, deserts, and wetlands [19; 20; 21; 22; 23].