## 3 Results and Discussion
### Coral Health Predictions from Field Data  
An exclusively field-based analysis was next undertaken in which replicate colonies from diverse genotypes at sites of varying oceanographies were sampled before, during, and after a bleaching event. The same BRCA2-rich protein discussed above that contributed significantly to variation across coral biopsy fragment health designations also contributed 36% to the overall temporal variation (see Supplementary File S2 for other univariate trends in situ). Given that its cellular concentrations in August were 50% lower (Table 4), this signifies that this currently unidentifiable protein must be prioritized by those interested in the cellular biology of this important Caribbean reef-builder. One could argue that it is only practical to validate the model with data from corals that have not yet bleached since this is how it would be used by a researcher or conservationist in the field; there would be no conservation merit in inputting data from an actively bleaching coral into the model since the bleaching susceptibility of that coral would inherently be known. The model's value would be in leveraging data from corals that have not yet bleached, particularly those that may be sub-lethally stressed but not yet manifesting visible signs of bleaching, and then determining whether these corals later bleached. Models trained with field data from pre-bleaching samples only (\"July data only(b)\" in Supplementary File S2) were characterized by accuracies of 92-93% with neural networks and support vector machines. Although this signifies that the AI could differentiate a bleaching-prone coral from a bleaching-susceptible one _before_ the former manifested any signs of bleaching, we were concerned about the timing since the models were validated with proteomic data from corals sampled only one month before the bleaching event began, \begin{table}
\begin{tabular}{l l l l l} \hline \hline \multirow{2}{*}{**Accession**} & \multirow{2}{*}{**Protein Name**} & \multicolumn{1}{c}{**Top BLAST Hit**} & \multicolumn{1}{c}{**CHD-Lab\(\to\)CHD-**} \\  & & \multicolumn{1}{c}{**Accession**} & \multicolumn{1}{c}{**Protein Function**} & \multicolumn{1}{c}{**Field(a) Model**} \\  & & & & **Total Effect** \\ \hline \multicolumn{5}{c}{**Proteins featured in model**} & \multicolumn{1}{c}{**CHD-lab\(\to\)CHD-field(a)** (Table 3)} \\ \hline OFAVBQ\_DN22242\_c2\_g1\_i4 & DELTA-activxin & **XP\_04182609.1** & prey capture & 0.44 \\ OFAVBQ\_DN225308\_c5\_g1\_i2 & E3 ubiquitin protein & **XP\_020616505.1** & protein degradation & 0.25 \\  & ligase & & & \\ OFAVBQ\_DN208218\_c2\_g1\_i13 & histone-lysine N- & **XP\_020616327.1** & transcription & 0.20 \\  & methyltransferase & & & \\  & SETD1B & & & \\ OFAVBQ\_DN225503\_c2\_g1\_i & b & concanavalin A-like & **XP\_020611325.1** & cell & 0.19 \\  & lectin/glucanase & & binding/immunity & \\ OFAVBQ\_DN207472\_c1\_g1\_i1 & histone H2A & **XP\_020604133.1** & chromatin & 0.18 \\ \hline \multicolumn{5}{c}{**Other proteins of interest**} & \multicolumn{1}{c}{**Finding**} \\ \hline OFAVBQ\_DN223604\_c1\_g1\_i2 \({}^{\text{c}}\) & unknown & **XP\_020618813.1** & unknown & 50\% lower in Aug. \\  & chromosome & **CAI3980295.1** & cell division & see Table 5. \\  & segregation protein & & & \\  & SMC & & & \\ OFAVBQ\_DN221258\_c1\_g2\_i8 & F-actin-methionine & **XP\_02060640.1** & molecular & see Table 5. \\  & sulfoxide oxidase & & trafficking & \\ OFAVBQ\_DN20777\_c1\_g3\_i5 & titin-like & **XP\_020600883.1** & various \({}^{\text{d}}\) & see Table 5. \\ OFAVBQ\_DN220189\_c1\_g1\_i3 & unknown & **XP\_020619646.1** & unknown & see Table 5. \\ OFAVBQ\_DN186152\_c\_g1\_i1 & transcription factor & **XP\_020624642.1** & transcription & see Table 5. \\ \hline \hline \end{tabular}  
* BLR \(>\) BLS (-50%; \(p=0.03\)). \({}^{\text{b}}\) AB \(>\) HC (1.5-fold) in lab samples [11]. \({}^{\text{c}}\) HC \(>\) all other fragment health designations. \({}^{\text{d}}\) Involved in musculature in vertebrates, though thought to play a role in chromatin in invertebrates.  
\end{table}