## Materials and Methods
### Experimental Procedure
#### 4.2.5 Determination of Antioxidant Capacity  
2,2-Diphenyl-2-Picrylhydrazyl (DPPH) Free Radical Assay  
DPPH assay of thyme extracts was determined according to previously reported procedure [38]. The results were calculated using calibration curve for Trolox and expressed as umol of Trolox equivalents per gram of samples (umol TE/g of sample).  
Ferric Reducing Antioxidant Power (FRAP) Assay  
The FRAP assay was conducted as previously reported [40]. FRAP values were calculated according to the calibration curve for FeSO4.7H2O and expressed as umol of Fe2+ equivalents (FE) per gram of sample (umol FE/g of sample).  
2.6 Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry Characterization of Phenolic Compounds (UPLC-MS/MS)  
Method for UPLC-MS/MS (Expert Ultra LC 110, Eksigent, Redwood city, CA, USA; 4500 QTRAP SCIEX, Redwood city, CA, USA) reference conditions [41] were conducted using a Luna Omega 3 um Polar C18 100 A, 100 x 4.6 mm column (YMC America, Inc., Allentown, PA, USA) at a thermostatted column temperature of 40 \({}^{\circ}\)C, automatic sampling temperature 4 \({}^{\circ}\)C, and injection volume 10 \(\upmu\)L using the original wild thyme extract concentration obtained by CE and HVED. Mobile phases consisted of: A 100% H2O with 0.1% HCOOH (_v/v_) and B 100% acetonitrile with 0.1% HCOOH (_v/v_) with mobile phase flow 0.40 mL/min. Gradient was set as follows: 1 min 10% B, 2 min 10% B, 15 min 90% B, 25 min 90% B, 27 min 10% B, 30 min 10% B. Determination conditions for MS/MS detector were: ionization -negative ionisation mode atmospheric pressure - negative ionization at atmospheric pressure; ionization temperature: 500 \({}^{\circ}\)C, i.e., gas temperature combining the mobile phase at the exit from the capillary before ionization; curtain gas 30 psi, temperature of 500 \({}^{\circ}\)C, ion source gas 1-50 psi, ion source gas 2-55 psi. Voltage on the electrode after capillary and next to ionization (ion spray voltage) was \(-\)4500 V. Limit of detection for all compounds was 0.10 ng/mL. A post-acquisition data processing was done in MultiQuant 3.0.2. Software, v3.022950.0, 2015.  
2.7 Headspace Solid-Phase Microextraction (HS-SPME) Followed by Gas Chromatography and Mass Spectrometry Analysis (GC-MS)  
HS-SPME was performed with manual SPME holder using three fiber covered with DVB/CAR/PDMS (Supelco Co., Bellefonte, PA, USA). For HS-SPME, the finely samples 2 mL were placed separately in 10 mL glass vials and hermetically sealed. The vials were maintained at 60 \({}^{\circ}\)C during equilibration (15 min) and extraction (45 min). Thereafter, the SPME fiber was withdrawn and inserted into GC-MS injector (250 \({}^{\circ}\)C) for 6 min for thermal desorption. The procedure was similar as reported [42]. GC-MS analyses were done on an Agilent 7890A Gas Chromatograph (Agilent Technologies, Palo Alto, CA, USA) equipped with a mass spectrometer (MSD) model 5977E (Agilent Technologies) and HP-5 MS capillary column (5% phenyl-methylpolysiloxane, AgilentJ & W). The GCconditions were same as reported previously by [PERSON] et al. (2016). The oven temperature was set at 70 \({}^{\circ}\)C for 2 min, then increased from 70 to 200 \({}^{\circ}\)C (3 \({}^{\circ}\)C/min) and held at 200 \({}^{\circ}\)C for 18 min; the carrier gas was helium (1.0 mL/min). The compounds identification was based on the comparison of their retention indices (RI), determined relatively to the retention times of n-alkanes (C9-C25), with those reported in the literature [43] and those from Wiley 9 (Wiley, New York, NY, USA) and NIST 14 (National Institute of Standards and Technology; Gaithersburg, MD, USA) mass spectral database. The percentage composition of the samples was computed from the GC peak areas using the normalization method (without correction factors).