## 3 Results
### GC-MS Analysis of Strain ess_amH1  
GC-MS was used to examine the natural products present in the EtoAc extract of the ess_amH1 strain and test for the existence of active chemicals. The NIST14 library was used to identify the molecular weight, structure, area percentage, and retention time (RT) of the natural compounds present in the extract. Fatty acids, phenols, aldehydes, alkaloids, and other chemicals were found at good to moderate quantities. Vital compounds found in the ethyl acetate extract of ess_amH1 isolate were E-2-decenal with RT = 8.9 min, 5-eicosene with RT = 11.4, hexadecanone with RT = 15.3, cyclohexane with RT = 16.2, pentadecanone with RT = 16.4, bis(3,5,5-trimethylhexyl) ether with RT = 17.9, benzenedicarboxylic acid with RT = 19.8, octacosane with RT = 22, and dodecane with RT = 31.6. The biological activities of the compounds are listed in Table 6. The chromatogram of the ess_amH1  
Figure 4: Phylogenetic analysis of _Streptomyces_ sp. strain ess_amH1. The phylogenetic tree was constructed on the basis of the 16s rRNA sequences using the maximum likelihood method and Tamura–Nei model in MEGA 11.0.13. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed.  
isolate revealed the relative concentration of the components (Figure 5). Each peak's height is relative to the amount of compound in the extract.  
\begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline
**S. No** & **Compound Name** & **RT** &
\begin{tabular}{c} **Molecular** \\ **Formula** \\ \end{tabular} & **M.W** & **Area (\%)** & **Applications** & **References** \\ \hline
1 & Cyclobutanol & 6.10 & C\({}_{4}\)H\({}_{3}\)O & 72.11 & 0.56 & Antibacterial & [23] \\
2 & Cyclohexane, butyl- & 6.64 & C\({}_{10}\)H\({}_{20}\) & 140.2 & 0.44 & Antimicrobial & [24] \\
3 & Undecane, 5,7-dimethyl- & 7.61 & C\({}_{13}\)H\({}_{28}\) & 184.3 & 0.41 & Antimicrobial & [25] \\
4 & E-2-Decenal & 8.96 & C\({}_{10}\)H\({}_{18}\)O & 154.2 & 3.40 & Antimicrobial & [26; 27; 28] \\
5 & Tetradecane & 9.08 & C\({}_{14}\)H\({}_{30}\) & 198.3 & 3.40 & Anticandidal & [29] \\
6 & Cyclohexane, 1,1’-(1,2-dimethyl)) & 9.20 & C\({}_{16}\)H\({}_{30}\) & 222.4 & 0.82 & Antimicrobial & [24] \\
7 & Cyclohexane, hexyl- & 9.69 & C\({}_{12}\)H\({}_{24}\) & 168.3 & 2.67 & Antimicrobial & [24] \\
8 & Oxiranemethanol & 10.0 & C\({}_{9}\)H\({}_{10}\)O\({}_{2}\) & 150.1 & 0.03 & Antibacterial & [30] \\
9 & 2-Undecanone & 10.3 & C\({}_{11}\)H\({}_{22}\)O & 170.2 & 1.52 & Antimicrobial & [25] \\
10 & 5-Eicosene & 11.4 & C\({}_{20}\)H\({}_{40}\) & 280.5 & 2.57 & Anticancer & [31] \\
11 & 3-Tetredecane & 11.7 & C\({}_{14}\)H\({}_{28}\) & 196.3 & 7.48 & Antimicrobial & [25] \\
12 & Tetredecane & 11.8 & C\({}_{14}\)H\({}_{30}\) & 198.3 & 5.01 & Antimicrobial & [25] \\
13 & Cyclohexane, 1,2,4,5-tetraethyl & 12.0 & C\({}_{14}\)H\({}_{28}\) & 196.3 & 0.67 & Antimicrobial & [24] \\
14 & Cyclohexane, octyl- & 12.7 & C\({}_{14}\)H\({}_{28}\) & 196.3 & 2.04 & Antimicrobial & [24] \\
15 & 1,1-Dichloro-2-dodecanol & 15.1 & C\({}_{12}\)H\({}_{24}\)Cl\({}_{2}\)O & 255.2 & 0.38 & Antifungal & [32] \\
16 & Bis(3,5,5-trimethylhexyl) ether & 15.2 & C\({}_{18}\)H\({}_{38}\)O & 270.5 & 10.22 & Antifungal & [33] \\
17 & Hexadecane & 15.3 & C\({}_{16}\)H\({}_{34}\) & & & & \\ \hline \hline \end{tabular}
\end{table}
Table 6: Compound analysis by GC–MS for EtOAc extract of _Streptomyces_ sp. strain ess_amH1.