## 2 Results and Discussion
### Statistical Analysis
#### 2.3.1 Comparison of the Extraction Methods with the Solventless Method
\begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline \multirow{2}{*}{**Sample**} & \multirow{2}{*}{**Method with Solvents**} & \multicolumn{3}{c}{**Method without Solvent**} & \multirow{2}{*}{\(t\)} & \multirow{2}{*}{\(p\)} & \multirow{2}{*}{\(d\)**[PERSON]**} & \multirow{2}{*}{\(p\)**[PERSON]**} \\ \cline{2-2} \cline{6-7}  & & & & & & & \\ \cline{2-2} \cline{6-7}  & **Mean** & **SD** & **Mean** & **SD** & & & \\ \hline HD1 & 0.55 & 1.15 & 3.49 & 9.49 & 0.19 & 0.855 & 0.04 & 0.983 \\ HD2 & 0.46 & 0.77 & 3.49 & 9.49 & 0.53 & 0.603 & 0.12 & 0.872 \\ SLE1-1 & 1.25 & 0.53 & 9.16 & 16.25 & 0.35 & 0.787 & 0.25 & 0.655 \\ SLE1-2 & 0.55 & 0.57 & 9.16 & 16.25 & 0.92 & 0.409 & 0.41 & 0.225 \\ SLE1-3 & 0.48 & 0.44 & 7.92 & 15.19 & 1.02 & 0.353 & 0.42 & 0.345 \\ SLE2-1 & 0.76 & 0.08 & 17.73 & 20.96 & 1.77 & 0.327 & 1.25 & 0.180 \\ SLE2-2 & 0.87 & 0.39 & 17.73 & 20.96 & 2.09 & 0.284 & 1.48 & 0.180 \\ SLE2-3 & 0.73 & 0.59 & 17.73 & 20.96 & 2.67 & 0.228 & 1.89 & 0.180 \\ SLE3-1 & 0.85 & 0.04 & 16.49 & 22.22 & 1.65 & 0.347 & 1.17 & 0.180 \\ SLE3-2 & 0.90 & 0.07 & 16.49 & 22.22 & 1.64 & 0.349 & 1.16 & 0.180 \\ SLE3-3 & 0.91 & 0.08 & 16.49 & 22.22 & 1.64 & 0.349 & 1.16 & 0.180 \\ SLE4-1 & 0.74 & 0.52 & 13.40 & 19.18 & 2.26 & 0.109 & 1.13 & 0.068 \\ SLE4-2 & 0.64 & 0.43 & 13.40 & 19.18 & 1.68 & 0.153 & 0.69 & 0.116 \\ SLE4-3 & 0.57 & 0.41 & 13.40 & 19.18 & 2.01 & 0.101 & 0.82 & 0.116 \\ SLE5-1 & 0.59 & 0.36 & 13.46 & 19.13 & 1.45 & 0.283 & 0.84 & 0.109 \\ SLE5-2 & 0.74 & 0.39 & 10.77 & 17.62 & 1.40 & 0.297 & 0.81 & 0.109 \\ SLE5-3 & 0.74 & 0.39 & 10.77 & 17.62 & 1.40 & 0.297 & 0.81 & 0.109 \\ MAE1 & 4.47 & 9.14 & 9.67 & 15.96 & \(-\)0.96 & 0.373 & 0.36 & 0.398 \\ MAE2 & 3.74 & 6.63 & 10.73 & 17.61 & \(-\)1.04 & 0.339 & 0.39 & 0.612 \\ MAE3 & 3.29 & 8.34 & 10.85 & 17.56 & \(-\)0.63 & 0.555 & 0.24 & 0.398 \\ MAE4 & 3.13 & 7.35 & 10.91 & 17.52 & \(-\)0.80 & 0.448 & 0.28 & 0.889 \\ \hline \hline \end{tabular}
\end{table} 
Table 7: Analysis of the differences between the solvent-free method and individual extraction methods among unidentified compounds. MAE4) the severity of sesquiterpene compounds was slightly lower compared to SPME (\(d>0.20\)). In the case of aromatic compounds (Table 5), no statistically significant differences were found under these comparable conditions. However, it was observed that only in the MAE method, Cohen d values greater than 0.20 were obtained, indicating that there was no possible difference between the means. On the other hand, in the case of HD and SLE, medium-sized difference effects (\(d>0.50\)) were found under the tested conditions, which showed a possibly higher level of aromatic compounds compared to the solvent-free method. As in aromatics, the samples tested for sesquiterpenoid compounds did not show statistically significant differences in the SPME condition (Table 6). However, the differences between the measurements were found to be at least weak in each case (\(d>0.20\)). Observing the averages, it was found that a higher level of volatiles is possible in the SLE and MAE for the conditions HD1, HD2, and MAE2 compared to the solventless method. For the MAE conditions, SLE1-2 and SLE1-3 showed lower levels of sesquiterpenoid compounds compared to the SPME condition, while the conditions SLE3-1, SLE3-2, and SLE3-3 showed potentially higher levels of sesquiterpenoid compared to the solvent-free condition. As in the case of general results, no statistically significant differences were found between the individual extraction methods compared to the solvent-free method in the sample of unidentified compounds (Table 7). However, a biased effect of the difference between SPME and SLE4-1 (\(p=0.068\)) was found, indicating a higher level of unidentified compounds in the SPME trial. Furthermore, it was noticed that only in the case of the HD method, [PERSON]'s d index allowed us to find no difference between the solvent-free method and the HD method (\(d<0.20\)).