## 3 Results
Regarding inoculation, plots where grasses were inoculated in the crop rotation showed 14.1% and 12.8% lower MV and MS of Piata grass, respectively, compared to non-inoculated plots, as indicated in Table 10.  
Table 13 presents the chemical soil attributes for the 0.00-0.10 m layer, where significant interactions were identified between phosphorus rates and microbial inoculation for phosphorus (P), calcium (Ca), and aluminum saturation (m%). Notably, P and Ca levels were higher in non-inoculated plots, particularly at 120 and 240 kg ha\({}^{-1}\) of P\({}_{2}\)O\({}_{5}\). In contrast, m% was higher in inoculated plots at these same doses. These results reflect the contrasting roles of inoculation and P fertilization in nutrient dynamics and acid-base balance. Additionally, phosphorus and CEC values were classified as low and high, respectively, while OM content was at medium levels and pH remained low, as per the reference standards of Bulletin 200. Phosphorus content increased with higher P\({}_{2}\)O\({}_{5}\) rates. Inoculation with _A. brasilense_ resulted in slightly higher phosphorus levels compared to the non-inoculated treatment, especially at the lower fertilizer rates. The greatest difference was observed at the 240 kg ha\({}^{-1}\) rate, with 29.5 mg dm\({}^{-3}\) in the non-inoculated treatment and 11.2 mg dm\({}^{-3}\) with inoculation (Table 14). Calcium content was higher in the non-inoculated treatments, particularly at the intermediate P\({}_{2}\)O\({}_{5}\) rates (30 and 60 kg ha\({}^{-1}\)). Inoculation with _A. brasilense_ led to lower calcium levels across all fertilizer doses (Table 15). It is noteworthy that for both P and Ca contents, no regression adjustments were found for phosphorus rates in areas with or without _A. brasilense_ inoculation. Regarding aluminum saturation (Table 16), higher means were observed in non-inoculated plots at P\({}_{2}\)O\({}_{5}\) rates of 0 and 60 kg ha\({}^{-1}\). In inoculated areas, higher means were recorded at P\({}_{2}\)O\({}_{5}\) rates of 30, 120, and 240 kg ha\({}^{-1}\), again without a significant regression model within the breakdown. For the inoculation factor, sulfur (S), calcium (Ca), magnesium (Mg), potential acidity (H+Al), sum of bases (SB), base saturation (V%), organic matter (OM), and hydrogen potential (pH) did not show significant differences. In contrast, phosphorus (P), potassium  
\begin{table}
\begin{tabular}{l c c c c c c c c c c c} \hline \hline
**Treatment** & **P** & **S** & **K** & **Ca** & **Mg** & **H+Al** & **BS** & **CEC** & **V** & **m** & **OM** & **pH** \\  & **mg dm\({}^{-3}\)** & & & & & **mmol**, dm\({}^{-3}\) & & & **\%** & & **g dm\({}^{-3}\)** & **(CaCl\({}_{5}\))** \\ \hline \multicolumn{10}{c}{P\({}_{2}\)O\({}_{5}\) doses (P)} \\ \hline
0 & 10 & 5 & 4.1 & 25 & 17 & 33 & 46.4 & 79.3 & 59 & 4 & 28 & 5.1 \\
30 & 13 & 5 & 4.1 & 28 & 19 & 33 & 51.2 & 83.7 & 60 & 4 & 28 & 5.1 \\
60 & 11 & 5 & 4.2 & 23 & 16 & 33 & 44.2 & 77.8 & 57 & 4 & 28 & 5.1 \\
120 & 12 & 5 & 4.1 & 23 & 15 & 33 & 42.3 & 75.2 & 56 & 6 & 27 & 5.1 \\
240 & 20 & 5 & 4.1 & 28 & 17 & 34 & 47.1 & 81.0 & 58 & 4 & 29 & 5.1 \\ \hline \multicolumn{10}{c}{Inoculation (I)} \\ \hline Without & 16 a & 5 & 4.6 a & 26 & 18 & 33 & 48.9 & 82.2 a & 59 & 4.1 b & 29 & 5.1 \\ With & 11 b & 5 & 3.8 b & 24 & 16 & 33 & 43.6 & 76.8 b & 57 & 4.8 a & 28 & 5.1 \\ \hline Test\({}^{-}\)P & 0.0001 \({}^{**}\) & 0.986\({}^{**}\) & 0.962\({}^{**}\) & 0.102\({}^{**}\) & 0.483\({}^{**}\) & 0.976 \({}^{**}\) & 0.545 \({}^{**}\) & 0.259 \({}^{**}\) & 0.886 \({}^{**}\) & 0.007 \({}^{**}\) & 0.311 \({}^{**}\) & 0.948 \({}^{**}\) \\ \hline Test\({}^{-}\)I & 0.0001 \({}^{**}\) & 0.283\({}^{**}\) & 0.000 \({}^{**}\) & 0.072 \({}^{**}\) & 0.25\({}^{**}\) & 0. (K), and cation exchange capacity (CEC) increased in non-inoculated areas, whereas only aluminum saturation (m%) showed higher means in the inoculated area.