## 3 Results
### Effects of TJ on Cell Viability in 3T3-L1 Cells
Moreover, PC1 (1299.7 \(\pm\) 63.5 pmol/mL), PC2 (1775.0 \(\pm\) 69.1 pmol/mL), and TJ groups (10: 685.7 \(\pm\) 50.0 pmol/mL, 50: 936.0 \(\pm\) 67.4 pmol/mL, 100: 1153.3 \(\pm\) 45.4 pmol/mL, 200: 1320.3 \(\pm\) 58.1 pmol/mL, respectively) showed significant increases compared to that of the DC group (Figure 6A). The intracellular FA levels of PC1 (282.1 \(\pm\) 19.2 nmol/uL), PC2 (170.0 \(\pm\) 2.1 nmol/uL), and TJ groups (10: 266.5 \(\pm\) 6.1 nmol/uL, 50: 243.3 \(\pm\) 10.5 nmol/uL, 100: 220.8 \(\pm\) 10.9 nmol/uL, 200: 196.5 \(\pm\) 4.5 nmol/uL, respectively) significantly decreased compared to that of the DC group (282.1 \(\pm\) 19.2 nmol/uL) (Figure 6B). As a result of testing the TG content, we found that the level of the DC group (13.0 \(\pm\) 0.9 mM) significantly increased compared to that of the NC group (2.9 \(\pm\) 0.3 mM). On the other hand, in the PC1 (8.4 \(\pm\) 0.5 mM), PC2(6.4 \(\pm\) 1.1 mM), and TJ groups (10: 12.4 \(\pm\) 0.4 mM, 50: 11.8 \(\pm\) 0.4 mM, 100: 10.1 \(\pm\) 0.1 mM, 200: 8.9 \(\pm\) 0.6 mM, respectively), they significantly decreased relative to that of the DC group (Figure 6C). Upon testing the glycerol release, we found that in the PC1 (0.96 \(\pm\) 0.08 ug/mL), PC2 (1.14 \(\pm\) 0.05 ug/mL), and TJ groups (10: 0.23 \(\pm\) 0.03 ug/mL, 50: 0.41 \(\pm\) 0.13 ug/mL, 100: 0.61 \(\pm\) 0.05 ug/mL, 200: 0.88 \(\pm\) 0.08 ug/mL, respectively), they significantly increased relative to that of the DC group (0.20 \(\pm\) 0.04 ug/mL) (Figure 6D). The TJ Inhibited the Lipid Accumulation Related to the Lipolysis Mechanism in Differentiated 3T3-L1 Cells  
As a result of measuring the lipid accumulation on the process of lipolysis in differentiated 3T3-L1 cells by conducting Oil Red O staining, we confirmed that the OD value of the DC group (1.31 \(\pm\) 0.02) significantly increased relative to that of the NC group (0.07 \(\pm\) 0.01). Furthermore, the result of the PC1 (0.87 \(\pm\) 0.05), PC2 (0.56 \(\pm\) 0.07), and TJ groups (10: 1.10 \(\pm\) 0.10, 50: 0.95 \(\pm\) 0.04, 100: 0.77 \(\pm\) 0.05, 200: 0.68 \(\pm\) 0.07) significantly decreased in a dose-dependent manner relative to the DC group (Figure 7A,B). Figure 6: The effects of the TJ in the cAMP level (**A**), intracellular FA (**B**), TG (**C**), and glycerol release (**D**) related to the lipolysis mechanism in differentiated 3T3-L1 cells. NC, normal control; DC, differentiated control; PC1, catechin 100 ug/mL; PC2, catechin 200 ug/mL; TJ (10, 50, 100, or 200 ug/mL). The data are shown as mean \(\pm\) standard deviation (SD; n = 3), and statistical analyses were conducted by [PERSON]’s multiple range tests after one-way ANOVA. Each lowercase alphabet indicates within-figure differing significance (\(p<\) 0.05). The TJ Regulated the Protein Expression Levels Related to the Lipolysis Mechanism in Differentiated 3T3-L1 Cells  
Upon measuring the protein expression level related to the mechanism of lipolysis, the phospho-AMPK/AMPK ratio was significantly increased in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (0.62 \(\pm\) 0.09, 1.07 \(\pm\) 0.06, 1.38 \(\pm\) 0.08 and 3.00 \(\pm\) 0.14, respectively) in a dose-dependent manner relative to that of the DC group (0.72 \(\pm\) 0.15) (Figure 8A,B). The UCP1/actin ratio was significantly increased in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (0.65 \(\pm\) 0.05, 0.87 \(\pm\) 0.07, 1.25 \(\pm\) 0.05 and 1.87 \(\pm\) 0.11, respectively) in a dose-dependent manner relative to that of the DC group (0.63 \(\pm\) 0.05) (Figure 8A,C). The PKA/actin ratio was significantly increased in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (1.28 \(\pm\) 0.05, 1.36 \(\pm\) 0.06, 1.38 \(\pm\) 0.06, and 1.58 \(\pm\) 0.11, respectively) relative to that of the DC group (0.32 \(\pm\) 0.05) (Figure 8A,D).