## 4 Conclusions  
The seven edible mushrooms addressed in this investigation showed substantial levels of protein, fiber, selected vitamins, and minerals as well as low amounts of reducing sugars and fat. These native foods contained fatty acids and phenols with proven nutraceutical activity. The phenol content, antioxidant capacity, and inhibitory activity of hydroalcoholic extracts obtained from the studied mushrooms on target enzymes linked to lipid, glucose, and cell proliferation demonstrate their potentiality as natural agents to prevent or control diseases associated with the metabolic syndrome, diverse types of cancer, as well as to maintain the equilibrium of gut microbiota. The results of the PCA and OPLS-DA models suggested that two groups of edible mushrooms might exert similar biological effects. Group 1 (_R. brevipes, C. squammulosa_, and _A. jacksonii_) had outstanding amounts of _p_-hydroxybenzoic acid, _p_-coumaric acid, vitamin C, and substantial levels of phenols. Group 2 (_R. delica, C. subclavipes_, C. _unda_, and _L. indigo_) had remarkable anti-lipase activity as well as substantial levels of Mg, thiamine, riboflavin, and oleic acid. This research could be useful for more profound studies focused on the evaluation of bioactive molecules from these edible sources and their mechanisms of action using in vitro and in vivo models. Figure 9: Discriminant statistical analysis for the edible mushrooms _Lactarius indigo_, _Clitocyte nuda_, _Clitocyte subclavipes_, _Russula delica_, _Russula brevipes_, _Clitocyte squamous_, and _Anamita jacksonii_. (**A**) principal component analysis. (**B**) orthogonal partial least squares discriminant analysis. (**C**) validation of orthogonal partial least squares discriminant analysis (R\({}^{2}\) = 1 and Q\({}^{2}\) = 0.997). (**D**) S-plot of orthogonal partial least squares discriminant analysis. (**C**) validation of orthogonal partial least squares discriminant analysis showing the dispersion of studied variables. **Supplementary Materials:** The following supporting information can be downloaded at: [[https://www.mdpi.com/article/10.3390/app14062520/s1](https://www.mdpi.com/article/10.3390/app14062520/s1)]([https://www.mdpi.com/article/10.3390/app14062520/s1](https://www.mdpi.com/article/10.3390/app14062520/s1)), Table S1: Macronutrient profile in seven mushrooms consumed in the northeastern highlands of Puebla-Mexico; Table S2. Vitamin content in seven mushrooms consumed in the northeastern highlands of Puebla-Mexico; Table S3. Mineral content in mushrooms consumed in in the northeastern highlands of Puebla-Mexico. Table S4. Nutraceuticaltical content in seven mushrooms consumed in the northeastern highlands of Puebla-Mexico; Table S5. Fatty acids content in seven mushrooms consumed in the northeastern highlands of Puebla-Mexico. Table S6. Total phenolics and antioxidant capacity of seven mushrooms consumed in the northeastern highlands of Puebla-Mexico. Table S7. IC\({}_{50}\) for the ethanolic extracts from edible organs of seven mushrooms consumed in the northeastern highlands of Puebla-Mexico. Figure S1. HPLC profiling of the hydroalcoholic extract from _Lactarius indigo_. 1, \(p\)-coumaric acid. 2, protocatechuric acid. 3, \(p\)-hydroxybenzoic acid. 4, cinnamic acid. IS, procyanidin B2 as internal standard. Figure S2. GC-MS profiling of the petroleum ether extract from _Lactarius indigo_. 1, TMS-linoleic acid. 2, TMS-oleic acid. 3, TMS-linolenic acid. IS, abietic acid as internal standard. Figure S3. Variable importance plot (VIP) plot for discriminate analysis for the parameters of _L. indigo_. Figure S4. Variable importance plot (VIP) plot for discriminate analysis for the parameters of _C. nuda_. Figure S5. Variable importance plot (VIP) plot for discriminate analysis for the parameters of _C. subclavipes_. Figure S6. Variable importance plot (VIP) plot for discriminate analysis for the parameters of _R. delica_. Figure S7. Variable importance plot (VIP) plot for discriminate analysis for the parameters of _R. brevipes_. Figure S8.