compared the toxicity of _spinosad_ and _cypermethrin_, and the results showed that the LC50 (24 h) of 25 g/L _spinosad_ SC was 1.341 \(\times\) 10\({}^{-5}\) mg/cm\({}^{2}\), with a safety factor of 0.054, and the LC50 (24 h) of 4.5% \(\beta\)_-cypermethrin_ emulsion in water is 2.711 \(\times\) 10\({}^{-5}\) mg/cm\({}^{2}\), with a safety factor of 0.120 [20]. There was also a study to measure the toxicity of _polyoxin_, _kasugamycin_, and _difenoconazole_, and the results showed that the EC50 values of _polyoxin_ and _kasugamycin_ were 60.184 ug\(\cdot\)mL\({}^{-1}\) and 86.438 ug\(\cdot\)mL\({}^{-1}\), respectively, which were significantly lower than that of _difenoconazole_ 4.239 ug\(\cdot\)mL\({}^{-1}\)[21]. Fourthly, the application of biopesticides is difficult to produce resistance. Comparing the control effects of _matrine_ AS and _bifentrin_ EC on tea looper (ectropis obliqua Prout), it was found that 3 d after application, the population reduction rate was 99.23% and 91.69%, respectively [22]. When the new biological mitoticide NBIF-001 wettable powder was used for 1 d, the adult mite body of Panonychus citri McGregor underwent lysis, and when the agent was used for 5 d, there were only insect residues on the leaves. In contrast, the Panonychus citri McGregor died after 5 d of using abamectin-spirodiclofen suspension concentrate, and the insects did not show cleavage, but the eggs increased, indicating that the Panonychus citri McGregor may have developed resistance to abamectin-spirodiclofen [23]. In addition, biopesticides have much fewer adverse effects on the soil. The diversity of soil microorganisms is the basis for maintaining healthy and stable agroecosystems. The effect of pesticides on soil microorganisms has become an important indicator for evaluating the ecological safety of pesticides in many countries. Take _Bacillus thuringiensis_ (Bt) as an example, Bt protein will be undetectable in soils such as yellow-brown, red, and brick red soils for two weeks [24], and it also showed a gradual decrease in other soils [25]. There were no significant changes in bacteria and fungi in the soil where Cry1 Ac protein had been applied within three months, and the diversity and homogeneity of microorganisms in the soil were not affected [26]. Furthermore, biopesticides have much less impact on other organisms in the ecosystem. For example, the field application of the Bt formulation XenTari did not affect the population of predatory and parasitic natural enemies [27]. Even directly feeding of Cry1 Ab protein had no negative effect on the growth and development of chrysopa perla [28]. Feeding high doses of Cry1 Ab/Ac protein to mice resulted in a rapid degradation in gastric and intestinal fluids, with no adverse effects on growth [29]. Volunteers received 1 g of Bt preparation (at a dose of 1011 CFU) for 5 d orally without adverse effects. At and below this dose, Bt and its products were not pathogenic or toxic to humans [30]. Biopesticides fit the development trends of modern agriculture and are gradually becoming mainstream in modern pesticide development. However, biopesticide production has long been restricted by prominent bottlenecks such as restricted resources and supplies, cumbersome production processes, and excessive resource consumption [31]. The rapid development of synthetic biotechnology in recent years has provided the possibility to break through these bottlenecks. Synthetic biology belongs to an emerging field that aims to transform our ability to detect, manipulate, and interact with living systems by combining knowledge and techniques from biology, chemistry, computers, and other disciplines [32]. In the past two decades, synthetic biology has seen significant developments, including the design of minimal bacterial genomes by [PERSON] et al. [33], the highly modified brewer's yeast genome by [PERSON] et al. [34], the biosynthesis of the antimalarial drug precursor _artemisitic acid_[35], and the yeast metabolic engineering of opioids such as _tiobain_ and _hydrocodone_[36].