###### Abstract  
Hops (_Humulus Inpuulus_ L.) are used traditionally in the brewing industry to confer bitternes, aroma, and flavor to beer. However, in recent years, it has been reported that female inflorescences contain a huge variety of bioactive compounds. Due to the growing interest of the consumers by natural ingredients, intense research has been carried out in the last years to find new sources of functional molecules. This review collects the works about the bioactive potential of hops with applications in the food, pharmaceutical, or cosmetic industries. Moreover, an overview of the main extraction technologies to recover biomolecules from hops is shown. Bioactivities of hop extracts such as antibacterial, antifungal, cardioprotective, antioxidant, anti-inflammatory, anticarcinogenic, and antiviral are also summarized. It can be concluded that hops present a high potential of bioactive ingredients with high quality that can be used as preservative agents in fresh foods, extending their shelf life, and they can be incorporated in cosmetic formulation for skincare as well. Keywords:hop; emerging extraction technologies; bioactivities; functional molecules +
Footnote †: journal: Applied Soft  
of the spasms, anxiety, fever, inflammation, activation of the gastric function, and the treatment of sleeping disorders, among others [2; 9]. Indeed, hops present numerous benefits for human health due to their antibacterial, antifungal, cardioprotective, antioxidant, anti-inflammatory, anticarcinogenic, and antiviral bioactivities [2; 9; 10; 11; 12; 5; 13]. The female infloreses (called hop cones or simply hops) as well as other parts of the plant (leaves, stems, and rhizomes) are rich in different biologically active molecules as polyphen compounds and acyl phloroglucides, which are responsible for the different health-promoting effects and bioactivities [3; 9]. The lupulin glands, which present a yellow-green color, are located on the hop umbel (in the inner and outer bracteoles) and contain bitter resins and aroma substances [1]. The secondary metabolites of the female infloreses (presented in the lupulin glands) can be divided into three groups: (1) the hop resins, (2) the hop oil, and (3) the hop polyphenols [14]. The levels of the aromatic essential oil and the bitter hop \(\alpha\)- and \(\beta\)-acids depend on several factors such as the variety or ripening stage and even on climatological conditions [15]. The main use of hops worldwide, around 97%, is used for brewing purposes [16] to add bitterness, aroma, and taste to the beer. Taking the above into account, and the current trend of searching natural compounds with novel biological properties, the wealth of the hop in bioactive compounds makes it an excellent source to extract biologically active molecules with multiple applications in the alimentary, pharmaceutical, and nutraceutical industries [12; 17; 18; 19; 20]. This has promoted intense research in the field of bioactive compounds from hops that allows on the one hand achieving high yields and on the other hand keeping the bioactivities of the extracted compounds. For this, the selection of adequate extraction technology as well as the appropriate solvent is fundamental. In this context, many publications related to the isolation of the active compounds have been developed over the past decades [5]. Several isolation technologies are well established using conventional (organic) solvents [5; 21]; nevertheless, their residual levels must be controlled [21], because they can remain in the final product and have detrimental health effects [5]. According to Marriott [21], EU legislation and organic certification bodies establish the allowed extraction solvents, their maximum residue levels, and further restrictions, respectively. To overcome the shortcomings of the conventional solvents [22], eco-friendly solvents such as natural deep eutectic ones represent a safer alternative, and their use has increased in the last decades [23].