## 3 Results and Discussion
### Enzyme Inhibition Potential of Corn Silk
There was previous research done on CS where _ent_-kaurane diterponoids were found, molecules such as kauralexin B1-B3 or kauralexin A1-A3, chemical entities with beneficial effects in Alzheimer's, as they were proved to manifest neuroprotective effects [57; 58], so testing the inhibition of acetylcholinesterase was justified. As these compounds are non-polar, we did not expect to have these molecules extracted, but we did get results regarding AChE inhibition, so this proves a plus in effect when talking about CS benefits in neurological disorders. In our study, we have been able to identify only weak activity against AChE, with IC\({}_{50}\) values of 1166.4 \(\pm\) 399.38 and 1222.67 \(\pm\) 285.32 \(\upmu\)g/mL for MSE50 and MSE70, respectively. There was no significant statistical difference between these two samples (\(p>0.05\)). Pancreatic lipase (PL) is an enzyme released into the duodenum that is crucial for the breakdown and assimilation of fats. In fact, PL is believed to be responsible for hydrolyzing 50-70% of dietary fats. Considering the pivotal role of PL in lipid digestion, it \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Enzyme** & **Control** & **MSI** & **MSE50** & **MSE70** & **MSO** \\ \hline \(\alpha\)-glucosidase & 52.04 \(\pm\) 2.30 & 1101.33 \(\pm\) 36.46 \({}^{\text{c}}\) & 451.6 \(\pm\) 7.86 \({}^{\text{b}}\) & 401.67 \(\pm\) 20.84 \({}^{\text{a}}\) & 411.67 \(\pm\) 25.39 \({}^{\text{a}}\) \\ Lipase & 68.17 \(\pm\) 3.74 & 10,079.33 \(\pm\) 581.97 \({}^{\text{b}}\) & 1508 \(\pm\) 145.08 \({}^{\text{a}}\) & 1535.67 \(\pm\) 72.43 \({}^{\text{a}}\) & 1519.67 \(\pm\) 209.04 \({}^{\text{a}}\) \\ AchE & 2.31 \(\pm\) 0.14 & ND & 1166.4 \(\pm\) 399.38 \({}^{\text{a}}\) & 1222.67 \(\pm\) 285.32 \({}^{\text{a}}\) & ND \\ Tyrosine & 56.27 \(\pm\) 2.03 & ND & ND & ND & ND \\ \hline \hline \end{tabular}  
* Notes: Different lowercase letters signify the presence of significant statistical difference among corn silk extracts. ND = no data/no activity; control standards: acarbose (\(\alpha\)-glu), orlistat (Lip), galantamine (AChE), and kojic acid (Tyrosinase).  
\end{table} 
Table 3: IC\({}_{50}\) values (expressed as \(\upmu\)g of lyophilized powder/mL of solution) for the enzyme inhibitory activity for the four types of extracts. emerges as a promising target for preventing obesity. The assessment of lipase inhibition potential provided valuable insights into the enzyme-inhibitory activity of the four types of extracts. Orlistat, a known lipase inhibitor, exhibited an IC50 value of 68.17 \(\pm\) 3.74 \(\mu\)g/mL, establishing a baseline for comparison. In contrast, the natural extracts displayed varying degrees of inhibitory potential. The MSI extract demonstrated an IC50 value of 10,079.33 \(\pm\) 581.97, while MSE50, MSE70, and MSO extracts exhibited values of 1508 \(\pm\) 145.08, 1535.67 \(\pm\) 72.43, and 1519.67 \(\pm\) 209.04, respectively. These results underscore the potential of MSE50, MSE70, and MSO extracts as inhibitors of pancreatic lipase. However, the results are modest in comparison with orlistat. In an animal study that focused on the effects and mechanisms of action of high maysin corn silk extract on body weight and fat deposition in mice, the high-fat corn silk (HFCS) group, receiving a high-fat diet along with high maysin corn silk extract, demonstrated significant reductions in body weight compared to the high-fat (HF) group that did not receive the corn silk extract [59]. Furthermore, kidney fat and epididymal fat pad weights were also significantly lower in the HFCS group. These findings suggest a promising role for corn silk extracts in modulating lipid metabolism and combating obesity. Tyrosinase, a multi-copper enzyme with a widespread distribution across various organisms, holds significance in both melanogenesis and enzymatic browning processes. As a result, tyrosinase inhibitors are of interest for use in the cosmetics and medicinal fields as agents for depigmentation.