## 4 Discussion  
The aim of this study was to elucidate the distinctions in interactions between strains of microorganisms that are prevalent in the oral cavity and materials that are intended for utilisation in in vivo dental surgical augmentation procedures [52; 60]. Moreover, the authors have investigated the influence of nHAp and nFAp materials on fibroblast cell activity. In order to assess the utility of such biomaterials, it is essential to conduct research on the potential outcomes of bacterial reactions to the biomaterial in question [61; 62]. The research demonstrated that the three commonly occurring strains of microorganisms in the oral cavity, namely _S. mutans_, _L. rhamnus_ and _C. albicans_, exhibited distinct responses to the nHAp and nFAp materials during the course of the investigation. As seen in Table 2, during the initial 24-h period, the only microbial strains that exhibited the lowest CFU/mL values in the samples containing apatite materials were _S. mutans_ and _C. albicans_. In the case of _C. albicans_ and _S. mutans_ strains, the presence of nFAp samples over time generally acted as a more potent inhibitor than in the samples of nHAp, thus confirming the inhibitory function that F\({}^{-}\) ions have on specific strains of microorganisms [63; 64]. Nevertheless, even in _S. mutans_ strains, the initial four hours of contact with both apatite materials resulted in the increase in CFU aggregation when compared to the control group. When assessing the mean value of CFU/mL of the strains within the first 24 h, significantly higher results were shown for _L. rhamnus_ interacting with nFAp than in samples containing nHAp. It is important to underline that both nFAp and nHAp samples showed greater activity of _L. rhamnus_ when compared to the control group. The difference in adherence of the bacterial cells to apatite materials is negligible apart from _S. mutans_ strains, which accumulate on the surface of nHAp in more significant quantities than on nFAp material over time. A study of the CFU/mL values when compared between nHAp and nFAp materials and microbial strains revealed that the lowest values were observed in the case of the _C. albicans_ strains in the first 2 h of cultivation. \begin{table}
\begin{tabular}{c c c} \hline \hline
**Time/Material** & **TCPS N** & **TCPS R** \\ \hline D14 & & \\ \end{tabular}
\end{table} 
Table 13: Microscopic pictures of the TCPS control group on the Alizarin Red staining group without (N) and with (R) the differentiation medium after contact with M3T3 preosteoblastic cells after 14 days of cultivation, scale representing 50 \(\upmu\)m.  
The initial hours following the surgical procedure represent a critical period, as they are the most likely to result in the emergence of idiopathic complications. A surgical procedure is deemed a success if the initial hours following the procedure facilitate the proper healing of tissue [65], thereby preventing the occurrence of any complications, including a microbial infection. The results of the study indicated that microbial adhesion to the materials differed depending on the microbial strain [35; 66]. The adhesion was measured within the first 24 h of contact, and both nHAp and nFAp materials were tested accordingly and compared. When it comes to adhesion comparison between the assessed biomaterials, the biggest noticeable difference was observed in the _S. mutans_ strains at 24 h after contact with the biomaterials, where it was higher in the case of contact with nHAp (1.31 \(\times\) 10\({}^{10}\)) than in contact with nFAp (4.19 \(\times\) 10\({}^{7}\)). Initially, as seen in Table 2, the nFAp material exhibited higher adhesion than the nHAp material, but after 24 h, the nHAp material demonstrated significantly lower adhesion. The assessment of _C. albicans_ and _L. rhamns_ adhesion to the tested materials yielded comparable results, with _C.