## 3 Results
### Effect of Combined Magnetic Fields on ROS Generation by Neutrophils
#### 3.2.2 Water-Mediated Effect of Magnetic Fields on Neutroph
\"Experimental sample dilution to 1099 without shaking\"--dilutions were made under the GMF (outside the magnetic field induction device), but without shaking between dilution steps. As can be seen in Table 3, the addition of sample \"GMF at 1099 dilution\" that was pre-exposed to the CMF with the frequency of alternating component of 12.6 Hz to neutrophil suspension increased the intensity of cell chemiluminescence by more than 44%. However, the effect of serial dilution and shaking under the GMF conditions caused only insignificant reduction in stimulating effect of water pre-exposed to the CMF (\"Experimental\" sample): the effect decreased insignificantly approximately from 65% to 44% (\(p\) = 0.09307). However, if shaking between dilution steps was performed under \"magnetic vacuum\" (~10 nT) conditions (Sample \"Magnetic vacuum\" at 1099\"), the ability of such solution to stimulate cell chemiluminescence was significantly lower than that of \"Experimental\" sample (\(p\) = 0.00325) or \"GMF at 1099 dilution\" (\(p\) = 0.00433). The same differences were observed for the group \"Experimental sample dilution to 1099 without shaking\" which were lower than for \"Experimental\" sample (\(p\) = 0.00325) or \"GMF at 1099 dilution\" (\(p\) = 0.00433). To the former sample only dilutions with delicate mixing under the GMF conditions were applied, but without vigorous shaking between the steps. Therefore, both presence of non-zero magnetic field and shaking process are important for the ROS production increase effect implementation. Noteworthy, in the similar experiment performed at 48.5 Hz, no effect of dilution process was detected. Significant differences were detected neither for samples compared to \"Experimental\"sample (\(p\) = 0.6991 for each comparison) nor compared to \"GMF at 1099 dilution\" (\(p\) = 0.2403 for each comparison). At the next step, we evaluated whether effects of the samples shaken under the CMF conditions are different from those shaken under the CMF conditions. The experiment included both samples obtained under CMF at 12.6 Hz and at 48.5 Hz. We demonstrated (Table 4) that the effect of \"Experimental\" sample was still significantly higher than that of Control (\(p\) = 0.00217) at alternating component frequency of 12.6 Hz. Moreover, if shaking process was performed under CMF conditions at alternating component frequency of 12.6 Hz, the resulting sample increased neutrophil chemiluminescence (approximately by 36%) similarly to the sample incubated under the GMF conditions (an increase of about 43%) (\(p\) = 0.1797). However, none of such effects manifested at 48.5 Hz: neither \"Experimental\" sample differed from the Control (\(p\) = 0.4848), nor the CMF or GMF shaking conditions affected the neutrophil respiratory burst (\(p\) = 0.132) (Table 4). Meanwhile, lack of water pretreatment with the CMF (i.e., exclusion of preparation stage of \"Experimental\" sample) did not enable samples to stimulate cell chemiluminescence even after dilution (\(p\) = 0.3095 vs. Control) and vigorous shaking (\(p\) = 0.9307 vs. \"GMF without shaking\") (Table 5). 2.4 Water-Mediated Activation of Neutrophils Exposed to the Spectral-Formed CMF with Intensive Mechanical Exposure and Dilution  
According to the results presented in the previous sections, activating effect on production of ROS by neutrophils is exerted by water pre-exposed to the CMF, the parameters of which are comparable to the GMF intensity. An important role for the preparation of samples that are able to increase cell chemiluminescence when added to neutrophils belongs to both magnetic field intensity and frequency of its alternating component, where the most impressive results were obtained at the frequency corresponding to ICR of the hydrated form of hydronium ions (12.6 Hz) but not at H\({}_{3}\)O\({}^{+}\) frequency (48.5 Hz).