## 3 Results
### Regulation of Body Weight Gain by F2 on Ohese Mice  
In 5 weeks of the obesity induction period, body weight and weight gain of the high-fat diet-fed groups were found to be significantly increased compared to the standard diet-fed normal group (Figure 3A), confirming the successful development of obesity. After induction of obesity in three HFD fed groups, one group was treated with the blank (0.2% CMC/PBS) as a control (HFD control), and the other two groups were treated with the F2 (46 mg/kg) and GC (200 mg/kg), separately. Normal group was also treated with the blank. The weight gain during the obesity induction period in the F2 and GC groups were 6.25 \(\pm\) 1.16 g and 6.17 \(\pm\) 1.70 g, respectively. However, the weight gain for F2 and GC reduced to 3.71 \(\pm\) 1.29 g and 3.29 \(\pm\) 0.28 g, respectively, during the 7 weeks of drug treatment period (Table 4). The body weight gain of the HFD control group remained significantly higher compared to the normal group at the end of the experiment. F2 and GC-treated groups showed significant reduction in body weight gain in comparison to the HFD (Figure 3B). Furthermore, the FER, an indication of the amount of food utilized to gain body weight, was significantly reduced in the F2 and GC treated groups compared with the HFD control group (Table 4).  
\begin{table}
\begin{tabular}{c c c c c} \hline \hline  & **Normal** & **HFD** & **F2-46** & **GC** \\ \hline Initial body weight (g) & 19.35 \(\pm\) 1.02 & 20.02 \(\pm\) 1.29 & 20.23 \(\pm\) 1.03 & 19.67 \(\pm\) 0.63 \\ Body weight after 5-weeks of obesity & 21.85 \(\pm\) 1.22 ** & 26.06 \(\pm\) 1.10 & 26.49 \(\pm\) 1.84 & 25.84 \(\pm\) 2.01 \\ Induction period (g) & & & & \\ Body weight gain in 5-weeks of & & & & \\ obesity induction period (g) & 2.51 \(\pm\) 0.51 *** & 6.04 \(\pm\) 1.54 & 6.25 \(\pm\) 1.16 & 6.17 \(\pm\) 1.70 \\ Final weight after 7-weeks of drug & & & & \\ treatment period (g) & 23.97 \(\pm\) 1.36 *** & 31.70 \(\pm\) 1.14 & 30.20 \(\pm\) 2.53 & 29.14 \(\pm\) 2.00 \\ Weight gain during 7-weeks of drug & & & & \\ treatment period (g) & & & & \\ Food intake during 7-weeks of the & & 139.94 & 101.92 & 100.52 & 101.38 \\ drug treatment period (g /mice) & & & & \\ Food efficiency ratio (\%) & 1.51 \(\pm\) 0.35 *** & 5.53 \(\pm\) 0.99 & 3.69 \(\pm\) 1.28 ** & 3.25 \(\pm\) 0.28 *** \\ \hline \hline \end{tabular} The body weight and food intake were measured every week. FER was calculated as follows: FER (%) = body weight gain (g) \(\times\) 100/ food intake (g). Values are expressed as the mean \(\pm\) SD (n = 5 to 6). A one-way ANOVA was applied to calculate statistical significance. ** \(p\) < 0.01; and *** \(p\) < 0.001 vs. HFD control. Normal = standard diet; HFD = high-fat diet control; F2-46 = F2 (46 mg/kg); GC = G. _cambogia_ (200 mg/kg).  
\end{table}
Table 4: Diet-induced obesity and controlling of weight gain by F2 in obese mice.  
Figure 3: Effects of F2 on body weight gain. Mouse body weight was measured every week. Mice were fed with only HFD or standard diet for 5 weeks of the obesity induction period. Obesity was successfully developed in the HFD fed groups in 5-weeks. The obese mice were then treated with F2, GC, or vehicles (0.2% CMC/PBS) for another 7 weeks. The body weight gains (**A**) during the 5 weeks of the obesity induction period and (**B**) during the 7 weeks of drug treatment period are presented separately. Values are expressed as the mean \(\pm\) SD (n = 5 to 6). A one-way ANOVA was applied to calculate statistical significance. @@@ \(p\) < 0.001 vs. Normal; ** \(p\) < 0.01; and *** \(p\) < 0.001 vs. HFD control. Normal = standard diet; HFD = high-fat diet control; F2-46 = F2 (46 mg/kg); GC = G. _cambogia_ (200 mg/kg).