## 2 Results and Discussion
### Analysis of Cannabinoid Profiles of Extracts  
In the next stage of this study, the cannabinoid profiles of extracts obtained through magnetic stirrer-assisted extraction and pressurized extraction at 1 and 2 bar were analyzed using gas chromatography coupled with mass spectrometry (GC-MS). For pressurized extraction using less polar solvents such as acetone, hexane, and their mixtures, the highest cannabinoid fractions were achieved at the lower pressure of 1 bar, which allowed for a longer contact time between the solvents and the raw material bed. Conversely, for ethanol, methanol, and isopropanol, higher cannabinoid extraction efficiencies were observed at the higher pressure of 2 bar. The optimal pressure values for each solvent were determined experimentally by analyzing the residual cannabinoid content in the post-extraction material, ensuring that it was below 5.7% relative to the starting material. Table 1 presents the cannabinoid fractions in extracts obtained via pressurized extraction (at 1 or 2 bar) at temperatures of 25 \({}^{\circ}\)C and \(-\)55 \({}^{\circ}\)C. Due to the significant fragmentation of the plant material and concerns about the undesirable extraction of chlorophyll, a temperature of \(-\)55 \({}^{\circ}\)C was used for extraction. Moreover, according to the literature, using the mentioned solvents at extraction temperatures between \(-\)60 \({}^{\circ}\)C and \(-\)40 \({}^{\circ}\)C on a large scale allows for extracts of higher purity to be obtained [32]. Across all variants using different solvents, the cannabinoid profiles varied and ranged from 39.85% to 51.57%. The highest concentration of the cannabinoid fraction from the Future 75 variety was achieved through pressurized extraction (1 bar) using a hexane/ethanol solvent mixture at a 7:3 volumetric ratio and a temperature of 25 \({}^{\circ}\)C. Although the extraction yield under these parameters was not the highest (yielding only 0.29 g of extract, as shown in Table S1 in the Supplementary Materials), the extract contained the highest cannabinoid concentration of 51.57% (Table 1). For comparison, in the variant with the highest extraction yield (0.46 g of extract obtained using methanol at 2 bar and 25 \({}^{\circ}\)C), the cannabinoid fraction was the lowest, at 42.01% (Table 1). In extracts obtained through magnetic stirrer-assisted extraction, the highest cannabinoid fraction (48.76%) was achieved using hexane for 40 min at \(-\)55 \({}^{\circ}\)C (Figure 1). However, the yield of the extract in this case was significantly lower (0.20 g) compared to the extraction variant using methanol at 25 \({}^{\circ}\)C for 40 min, where the highest extract mass of 0.45 g was obtained, though the cannabinoid content was below 40%. Additionally, it was observed that prolonging the extraction time with methanol, ethanol, or acetone did not significantly increase the cannabinoid content. In contrast, increasing the extraction temperature led to a reduction in cannabinoid levels and an increase in undesirable components such as waxes and triacylglycerglycerols [32]. \begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline \multirow{2}{*}{**Solvent**} & **Temp.