## 3 Results
\begin{table}
\begin{tabular}{c c c c c c c c c c c} \hline \hline \multirow{2}{*}{**Forest Habitat**} & \multirow{2}{*}{**Forest**} & \multirow{2}{*}{**Tannins**} & \multirow{2}{*}{**Content [\%]**} & & \multicolumn{3}{c}{**Tannins**} \\  & & & & & **Tannins Content [\%]** & & & & **Content-Average** \\  & & & & & & & & & **per Forest Site [\%]** \\ \hline Moist mixed & 194d & 12.16 & 12.09 & 12.12 & 11.86 & 11.91 & 11.92 & 12.27 & 12.31 & 12.24 & \\ broadleaved forest & 187d & 13.77 & 13.58 & 13.52 & 13.85 & 13.98 & 14.02 & 13.54 & 13.44 & 13.24 & \\  & 186b & 14.42 & 14.50 & 14.38 & 14.34 & 14.29 & 14.22 & 14.25 & 14.16 & 14.31 & \\ \hline Fresh broadleaved & 224a & 14.75 & 14.76 & 14.55 & 15.05 & 15.11 & 14.95 & 14.83 & 14.79 & 14.90 & \\ forest & 164a & 13.22 & 13.10 & 13.15 & 13.08 & 13.09 & 12.95 & 13.23 & 13.40 & 13.34 & 14.06 \\  & 153a & 14.24 & 14.32 & 14.26 & 14.19 & 13.99 & 14.10 & 14.11 & 13.97 & 14.30 & \\ \hline Moist broadleaved & 170i & 15.56 & 15.61 & 15.42 & 15.95 & 15.80 & 15.92 & 15.79 & 15.84 & 15.89 & \\  & 169i & 14.18 & 14.25 & 14.21 & 14.27 & 14.29 & 14.11 & 14.36 & 14.40 & 14.39 & 14.74 \\ forest & 140a & 14.12 & 14.10 & 14.01 & 14.11 & 14.28 & 14.19 & 14.29 & 14.19 & 14.42 & \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Content of tannins in _Cynips quercusfolii_ gall nuts. Figure 1: (**A**) The influence of forest habitat type on the content of tannins in _Cynips quercusfolii_ gall nuts (I, moist mixed broadleaved forest, II, fresh broadleaved forest, III, moist broadleaved forest); (**B**) The influence of the harvest date on the content of tannins in _Andricus infectorius_ gall nuts (I, August, II, September). Letters (a, b, c) are used to determine if statistically significant differences between factors exist. The same letter (for example, ‘a’) means there is no statistical difference. Figure 2: The mass spectrometric signature (-ESI SCAN) of: mixtures of gallic and tannic acid standards GA + TA (**A**) and representatives of the gall nuts samples from the groups _C. quercusfolii_ (**B**) and _A. infectious_ (**C**). \begin{table}
\begin{tabular}{c c c} \hline \hline
**Harvest Date** & **Tannins Content [\%]** &
\begin{tabular}{c} **Tannins Content-** \\ **Average per Term of Collection [\%]** \\ \end{tabular} \\ \hline \hline \multirow{7}{*}{I (August)} & 34.59 & 34.77 \\  & 34.39 & \\  & 34.60 & \\  & 34.74 & \\  & 34.62 & \\  & 34.70 & \\  & 34.95 & \\  & 35.11 & \\  & 35.21 & \\ \hline \hline \multirow{7}{*}{II (September)} & 40.47 & 39.95 \\  & 41.09 & \\  & 40.85 & \\  & 39.92 & \\  & 39.87 & \\  & 39.90 & \\  & 38.70 & \\  & 38.91 & \\  & 39.85 & \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Content of tannins in _Andricus infectious_ gall nuts. A infectorins gall nuts are rich in galloyl glucoses (from mono to octa-galloylglucose), compounds characteristic of the MS spectrum of tannic acid (Figure 2C). This is evidenced by the values of \((m/z)\) [M-H]\({}^{-}\) ions visible in MS spectra (169 gallic acid, 321 digallic acid, 33 galloylglucose, 483 digaloyl glucose, 635 trigaloyl glucose, 787 tetragaloyl glucose, 939 pentagaloyl glucose, 1091 hexagaloyl glucose, 1243 heptagaloyl glucose, 1396 octagaloyl glucose). In turn, _C. quercusfolii_ gall nuts contain quinic acid (in the [M-H]\({}^{-}\) MS spectrum); this is the parent ion with 191 mass \((m/z)\) (Figure 2B). These gall nuts contain parent ions with masses indicating the presence of ellagic acid 301 \((m/z)\) and gallic acid 169 \((m/z)\). These gall nuts are relatively poor in tannin compounds in the form of galloyl glucoses. The chromatographic profiles (DAD, MS) are a kind of \"tannin fingerprint\", clearly showing the profile of the tannin compounds in individual samples. The chromatograms (HPLC-MS-SCAN) (Figure 3) show the distribution of tannic compounds, galloyl glucoses in an aqueous solution of tannic acid, and in samples of Polish and Turkish gall nuts, they elute from 5 to 15 min, immediately after gallic acid (tr = 4 min).