## 3 Results
Regarding the inoculation factor, only K showed higher values in non-inoculated areas, while, in inoculated areas, only OM presented higher values. The remaining attributes were not significantly affected by the inoculation of grasses in rotation. \begin{table}
\begin{tabular}{c c c c c c c c c c c c c} \hline \hline
**Treatment** & **P** & **S** & **K** & **Ca** & **Mg** & **H+Al** & **BS** & **CEC** & **V** & **m** & **OM** & **pH** \\  & **mg dm\({}^{-3}\)** & & & & **mmol dm\({}^{-3}\)** & & & & **\%** & & **g dm\({}^{-3}\)** & **(CaCl\({}_{2}\))** \\ \hline \hline \multicolumn{11}{c}{P  
Thus, as shown in Table 17, plots where grasses were inoculated in the crop rotation had 10% lower K content compared to non-inoculated plots. On the other hand, for OM content, inoculated plots showed higher values, with an increase of 9.1%. Still within this layer, P content was considered low, CEC was high, OM was at a medium level, and pH was low. Contrasting the results of the two analyzed layers, it was observed that P, K, Ca, Mg, BS, CEC, V%, and OM contents decreased in deeper layers, whereas m% and pH showed increased values. S content increased with P\({}_{2}\)O\({}_{5}\) rates. Only S (with and without inoculation) and H+Al remained constant at both soil depths. When comparing the analysis of soil chemical attributes from the 2021/2022 and 2023/2024 growing seasons in the 0.00 to 0.10 m and 0.10 to 0.20 m layers, a similar behavior of soil elements was observed (Figure 8). \begin{table}
\begin{tabular}{l c c} \hline \hline \multicolumn{3}{c}{**Soil Aluminum Saturation in the 0.10 to 0.20 m Layer**} \\ \hline
**Treatment** & \multicolumn{2}{c}{**Inoculation**} \\ \hline
**P\({}_{2}\)O\({}_{5}\) Doses (kg ha\({}^{-1}\))** & **With** & **Without** \\ \hline  & \% & \\ \hline
0 & 5.0 B & 7.5 A \\
30 & 7.2 & 7.5 \\
60 & 6.5 & 7.5 \\
120 & 9.7 A & 7.5 B \\
240 & 3.7 B & 7.2 A \\ \hline \hline \end{tabular} Means followed by different uppercase letters in the row differed from each other according to the LSD post-hoc test at the 5% probability level.  
\end{table} 
Table 18: Breakdown of interactions—P\({}_{2}\)O\({}_{5}\) rates \(\times\) inoculation for the soil chemical attribute aluminum saturation in the 0.10 to 0.20 m layer in a no-till system, considering the residual effect of phosphate fertilization and the inoculation of preceding grasses with _A. brasilense_. Selvriia/MS, 2023/2024. Figure 7: Regression analysis for the breakdown of phosphorus rates within inoculation (non-inoculated) for soil P content in the 0.10 to 0.20 m layer in a no-till system, considering the residual effect of phosphate fertilization and the inoculation of preceding grasses with _A. brasilense_. Selvriia/MS, 2023/2024. There was an increase in P, K, V%, m%, and OM for both sources of variation and soil layers from the 2021/2022 season to the 2023/2024 season. For Ca levels and BS values, an increase was observed only in the 0.10 to 0.20 m layer, while, in the more superficial layer, a decrease occurred due to the treatments. It was observed that S, Mg, H+Al, and CEC decreased between seasons. The different P fractions were classified according to their liability in each extractor. Labile P included the Pi fractions extracted by RTA and the Pi and Po fractions extracted by sodium bicarbonate. Moderately labile P comprised the Pi and Po fractions extracted by 0.1 mol L\({}^{-1}\) NaOH, along with the inorganic P extracted with HCl. Meanwhile, non-labile phosphorus consisted of the sum of Pi and Po extracted by 0.5 mol L\({}^{-1}\) NaOH and residual Pi and Po. Table 19 presents the results of soil phosphorus fractionation in the 0.00-0.10 m layer. A significant interaction between phosphorus doses and inoculation was observed, particularly for labile and moderately labile phosphorus forms. In non-inoculated plots, an exponential increase was observed for both fractions with increasing P\({}_{2}\)O\({}_{5}\) doses, indicating a strong residual effect of fertilization.