###### Abstract  
The white oil-bearing rose (_R. alba_ L.) is the second of the industrially important rose species for Bulgarian rose cultivation and essential oil production. In recent years, the interest in white oil-bearing rose has increased, following the worldwide trend for searching for new aromatic alternatives. Therefore, the purpose of the current research is to evaluate the volatile compounds profile of fresh _R. alba_ L. flowers using headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC/MS). More than 75 individual compounds were identified and quantified using HS-SPME-GC/MS. The study revealed that the aroma-bearing fraction of rose volatiles consists mainly of monoterepene alcohols; 2-phenylethanol was the most abundant component (8.4-33.9%), followed by geraniol (12.8-32.5%) and citronello + nerol (17.7-26.5%). Linalool, \(\alpha\)-pinene, \(\beta\)-myrcene, and rose oxides were also observed in low concentrations. The steapropten fraction in the HS phase was observed in low concentration, with main representatives nonadecane + nonadecene, heptadecane, heneciosane, and tricosane. The HS-GC profile of the _R. alba_ fresh flowers shows distinct differences in relative abundance of the components between the two studied clones of the population, as well as between volatiles in petals and in the whole blossom. The absence of some undesirable components, such as allergenic and potentially carcinogenic methyl eugenol in fresh _R. alba_ blossom, makes white oil-bearing rose a promising alternative to _R. damascena_ in perfumery, natural cosmetics, and aromatherapy. R. alba L. Blossom by Headspace--Solid Phase Microextraction and Gas Chromatography. 2020. 3012. 33012. 33012.  
beneficial properties for perfumery, cosmetics, and medical applications, with a very low toxic potential [2; 4; 5]. This fact is due to the absence of some undesirable components, such as potentially allergenic methyl eugenol [1]. The industrial importance of oil-bearing roses is based on the extraction of the highly valued rose aroma from the rose flowers by a variety of methods (water steam distillation, solvent extraction, extraction with liquified gases, etc.). There is no doubt that the produced extracts (rose oil, rose concrete/absolute, rose sub/supercritical extracts) have aroma profiles different from the authentic one of the living flowers. This is caused by the niceties of the used technological procedures, and the best way to evaluate the changes in the aroma profile is to compare it with the aroma composition of the fresh, unprocessed flowers. Different parts of the flower emit different volatiles. Bearing in mind the high economic importance of the rose essential oil, it is valuable to reveal which volatile organic compounds (VOCs) are emitted by the flowers and which are a result of the hydro distillation process. Gas chromatography-olfactometry is one of the possibilities for such an investigation, but at the same time, it is limited by the needed specific equipment and the fact that it remains a subjective descriptive analysis. The headspace sampling, as a nondestructive technique, which can be used on living plants or freshly collected flowers, has been found to be the method of choice in many studies [6; 7; 8]. Several different headspace techniques (vacuum headspace technique, closed-loop stripping method, dynamic headspace technique, and solid-phase microextraction) [9; 10; 11] are commonly used to analyze plant VOCs. Among them, solid-phase microextraction (SPME), where various porous polymers are used as fiber materials, has been increasingly applied in the analysis of plant volatiles, including rose aroma. Compared with conventional extraction techniques, this method is convenient, easy to use, less time-consuming, and being solvent-free is truly green. In this way, HS-SPME provides one of the best platforms for sample preparation, especially for the analysis of volatile and semi-volatile organic compounds, which defines its wide practical use [9; 10; 11; 12; 13].