## 3 Results and Discussion
Similarly, [PERSON] et al. [78] suggested effectiveness of flaxseed oil against a broad spectrum of bacteria (e.g., _S. aureus_, _Streptococcus agalacticae_, _E. coli_, _E. faecalis_, and _M. luteus_) was with antimicrobial activity comparable to or better than the positive control (cefoperazone). According to [PERSON] et al. [79], flaxseed oil exhibited significant antibacterial activity against _Salmonella enteritidis_ and _S. typhimurium_ and no inhibitory effects against _S. aureus_, _L. monocytogenes_, and _B. cereus_. The same authors also suggested that cultivation practices may affect the bioactive properties of the obtained oils, with oils extracted from seeds of organically grown plants being more potent than those of conventionally grown ones [79]. This seems to be the case in our study, since linseed oil 3 (organic oil) was more effective against _E. coli_ than the other two linseed oils, while for the rest of the tested bacteria no differences were observed in MIC and MBC from linseed oil 2. Moreover, [PERSON] et al. [80] suggested the complementary use of flaxseed oil along with gemifloxacin to increase its effectiveness and reduce the development of resistance. The studies of antibacterial effects of luffa refer to aerial plant parts and seeds with limited research available on seed oil effects [42; 62]. According to [PERSON] et al. [81], luffa seed oils showed effectiveness against a wide range of bacteria (e.g., _S. aureus_, _S. epidermidis_, _Microoccus leutius_, _Pseudomonas aeruginosa_, and _E. coli_), while in all the cases the obtained MIC values were lower than the positive control used. Similarly, the reports for the antibacterial effects of purslane are limited, especially for seed oils [82; 83; 84; 85]. According to [PERSON] et al. [86], purslane seed extracts were only effective against normal _S. aureus_ strains, while [PERSON] and [PERSON] [87] also recorded effectiveness of leaf extracts against _S. aureus_, _B. aureus_, and _P. aeruginosa_. Recently, [PERSON] et al. [11] reported low effectiveness of purslane seed oil against different Gram+ and Gram- bacteria, although they suggested that raw seeds were more effective than seedcakes and seed oils, probably due to loss of bioactive compounds during the extraction procedure. In contrast, [PERSON] [88] found significant effectiveness of purslane fixed oil against _S. epidermidis_ and _E. coli_ and attributed this activity to the high content of omega-3 fatty acids. \begin{table}
\begin{tabular}{c c c c c c c c c c c c} \hline \hline \multirow{2}{*}{**Seed Oil**} & \multicolumn{2}{c}{_Staphylococcus aureus aureus_} & \multicolumn{2}{c}{_Macrococcus flavus (ATCC 1024)_} & \multicolumn{2}{c}{_Enterobacteraceae (ATCC 35030)_} & \multicolumn{2}{c}{_Salmonella_} & \multicolumn{2}{c}{_Escherichia coli (ATCC 25922)_} \\ \cline{2-11}  & **MIC** & **MBC** & **MIC** & **MBC** & **MIC** & **MBC** & **MIC** & **MBC** & **MIC** & **MBC** & **MIC** & **MBC** \\ \hline Linseed oil 1 & 2.00 & 4.00 & 1.00 & 2.00 & 1.00 & 2.00 & 1.00 & 2.00 & 1.00 & 2.00 & 1.00 & 2.00 \\ Linseed oil 2 & 4.00 & 8.00 & 1.00 & 2.00 & 1.00 & 2.00 & 0.50 & 1.00 & 2.00 & 4.00 & 2.00 & 4.00 \\ Linseed oil 3 & 2.00 & 4.00 & 1.00 & 2.00 & 1.00 & 2.00 & 1.00 & 2.00 & 1.00 & 2.00 & 0.50 & 1.00 \\ Luffa oil & 2.00 & 4.00 & 1.00 & 2.00 & 1.00 & 2.00 & 0.50 & 1.00 & 1.00 & 2.00 & 2.00 & 4.00 \\ Purslane oil & 2.00 & 4.00 & 2.00 & 4.00 & 2.00 & 4.00 & 2.00 & 4.00 & 2.00 & 4.00 & 1.00 & 2.00 \\ Cucurbit oil & 2.00 & 4.00 & 1.00 & 2.00 & 1.00 & 2.00 & 1.00 & 2.00 & 1.00 & 2.00 & 5.00 & 1.00 \\ \hline E211 & 4.00 & 4.00 & 0.50 & 0.50 & 1.00 & 2.00 & 2.00 & 4.00 & 1.00 & 2.00 & 1.00 & 2.00 \\ E224 & 1.00 & 1.00 & 2.00 & 4.00 & 1.00 & 2.00 & 0.50 & 0.50 & 1.00 & 1.00 & 0.50 & 1.00 \\ \hline \hline \end{tabular}  
* MIC: minimum inhibition concentration; MBC: minimum bactericidal concentration; E211: sodium benzoate; E224: potassium metabisulphite.  
\end{table} 
Table 3: Antibacterial activity of the studied seed oils (MIC and MBC mg/mL). Regarding pumpkin oil, [PERSON] et al.