## 3 Results and Discussion
### Bioactive Compounds in Raspberries Leaves  
The content of total polyphenols, carotenoids and anthocyanins as well as antioxidant activity of raspberry leaves from various kinds of cultivation different crops is shown in Table 7. \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Biactive Compounds** & **Cultivation** & **Conventional** & **Organic** & **Wild crop** & \\ \cline{2-6}
**Staircase** & **Year** & **2020** & **2021** & **2020** & **2021** & **2020** & **2021** \\ \hline Total polyphenols [mg CGA 100 g\({}^{-1}\) DW] & 2840.45 \(\pm\) 21.66 \({}^{\rm b}\) & 4030.79 \(\pm\) 36.80 \({}^{\rm d}\) & 1873.02 \(\pm\) 113.02 \({}^{\rm a}\) & 3559.10 \(\pm\) 100.62 \({}^{\rm c}\) & 5078.39 \(\pm\) 12.88 \({}^{\rm e}\) & 8785.07 \(\pm\) 303.74 \({}^{\rm f}\) \\ \hline Total polyphenols [mg GAE 100 g\({}^{-1}\) DW] & 2838.75 \(\pm\) 22.08 \({}^{\rm b}\) & 5130.35 \(\pm\) 39.76 \({}^{\rm d}\) & 2223.23 \(\pm\) 17.19 \({}^{\rm a}\) & 4396.18 \(\pm\) 110.37 \({}^{\rm c}\) & 5554.47 \(\pm\) 12.54 \({}^{\rm e}\) & 9675.66 \(\pm\) 314.94 \({}^{\rm f}\) \\ \hline ABTS\({}^{\rm m}\) [mmol Trolox g\({}^{-1}\) DW] & 67.21 \(\pm\) 0.09 \({}^{\rm a}\) & 802.49 \(\pm\) 5.65 \({}^{\rm b}\) & 70.86 \(\pm\) 0.07 \({}^{\rm a}\) & 852.32 \(\pm\) 3.28 \({}^{\rm c}\) & 72.79 \(\pm\) 0.28 \({}^{\rm a}\) & 988.63 \(\pm\) 1.37 \({}^{\rm e}\) \\ \hline FRAP [umol Trolox g\({}^{-1}\) DW] & 492.49 \(\pm\) 1.37 \({}^{\rm b}\) & 967.78 \(\pm\) 0.27 \({}^{\rm d}\) & 418.74 \(\pm\) 1.42 \({}^{\rm a}\) & 702.93 \(\pm\) 8.18 \({}^{\rm e}\) & 1223.34 \(\pm\) 40.01 \({}^{\rm e}\) & 1967.93 \(\pm\) 14.35 \({}^{\rm f}\) \\ \hline Total carotenoids [mg 100 g\({}^{-1}\) DW] & 82.81 \(\pm\) 4.38 \({}^{\rm d}\) & 73.41 \(\pm\) 2.95 \({}^{\rm c}\) & 57.64 \(\pm\) 3.36 \({}^{\rm d}\) & 58.89 \(\pm\) 2.56 \({}^{\rm b}\) & 78.53 \(\pm\) 3.89 \({}^{\rm c}\) & 49.78 \(\pm\) 2.31 \({}^{\rm a}\) \\ \hline \hline \end{tabular}  
* Results (from \(\pm\) SD) obtained from the analysis of three individual samples (\(\pm\) 3, 5). Values within lines with different letters are significantly different (‘‘‘‘‘).  
\end{table} 
Table 7: Total polyphenol, carotenoids and anthocyanins content as well as antioxidant activity of the leaves of three rasberries cultivation at different harvest (2020) and 2021. Raspberry leaves, regardless of their cultivation type, were characterized by up to five times the amount of total polyphenols, with respect to raspberry fruit, regardless of source. The highest statistically significant content was characterized by leaves of wild raspberries, in which the amounts were up to four times higher, compared to the content of these compounds in, for example, leaves of conventional raspberries from 2020. The lowest content of the these compounds was found in the leaves of organic raspberry collected in 2020, relative to the other samples analyzed. Antioxidant activity, as measured by the ability to quench the cation radical ABTS, was highest in leaves of wild raspberries from 2021, compared to the other studied raspberry fruits. The lowest and similar antioxidant activity was found in raspberry leaves from the 2020 harvest, regardless of the origin of the leaves. These results generally coincide with those of the FRAP antioxidant activity determination. The only exception is the significantly higher antioxidant activity in wild raspberry leaves from 2020 versus to the results for leaves from other sources. The greatest content of total carotenoids was found in conventional raspberry leaves from 2020 and 2021, compared to the other examined raspberry leaves. The lowest level of these compounds was observed in wild raspberry leaves from 2021. Noteworthy is the almost 100 times higher content of these compounds in raspberry leaves in some cases, in comparison to fruit, regardless of origin.