### Quality Parameters  
Data regarding the impact of different Zn formulations after the bio-activation and coating of ZnO on different quality parameters of wheat is presented in Table 1. Regarding ash (minerals) contents (%), the coating and bio-activation of Zn at the level of 1.5% resulted in the maximum increase compared to the control with no Zn application. The application of 1.5% bio-activated Zn coated urea and recommended Zn (ZnSO\({}_{4}\)) showed almost similar results regarding ash contents i.e., 6.06 and 5.70%, respectively. The treatments where  
Figure 4: Comparative effectiveness of different Zn formulations of urea regarding yield parameters of wheat (**a**) Biomass (tons/ha), (**b**) Grain yield (tons/ha), and (**c**) 100 grains weight. Where T\({}_{0}\) = no Zn; T\({}_{1}\) = recommended Zn (ZnSO\({}_{4}\)); T\({}_{2}\) = ZSB; T\({}_{3}\) = 1.5% Zn coated urea (ZnO); T\({}_{4}\) = 1.5% bio-activated Zn coated urea (ZnO); and T\({}_{5}\) = 1.5% Zn blended urea (ZnO). Means sharing the same letters do not differ significantly (\(p<0.05\)). In the case of oil contents, all treatments showed significant effects however, the maximum increase (55%) was observed with the application of 1.5% bio-activated Zn coated urea as compared to the control where no Zn was applied. Similarly, the application of 1.5% Zn coated and 1.5% Zn blended urea showed an increase of 39 and 21% in oil contents, respectively as compared to the control. In comparison to recommended Zn (ZnSO4), an increase of 33% was recorded with the application of 1.5% bio-activated Zn coated urea caused an increase of 23.4 and 66.7% in oil contents as compared to 1.5% Zn coated and 1.5% Zn blended urea, respectively. A statistically significant effect of different Zn formulations was observed in the case of nitrogen (N) contents in wheat grains (Table 1). It was clear from the data that the application of 1.5% bio-activated Zn coated urea showed 2.60% N contents, which were 29.65% more as compared to the control (no Zn). With the application of 1.5% Zn coated and 1.5% Zn blended urea, an increase of 24.18 and 23.86%, respectively in N contents was recorded compared to the control. The recommended Zn (ZnSO4) showed 2.50% N contents that were 1.8% more as compared to the control. As compared to recommended Zn (ZnSO4), 1.5% bio-activated Zn coated urea showed an increase of 5% in N contents. The application of 1.5% bio-activated Zn coated urea caused an increase of 7.8 and 8.2% in N contents as compared to 1.5% Zn coated and 1.5% Zn blended urea, respectively. The application of different Zn formulations was also tested for protein contents of wheat (Table 1). The data indicated that all treatments showed statistically significant effects (\(p<0.05\)) as compared to the control. The use of 1.5% bio-activated Zn (ZnO) coated urea was more effective and it caused an increase of 30% in protein contents compared to the control. It was followed by recommended Zn (ZnSO4) with 14.3%, 1.5% Zn coated with 13.9%, 1.5% Zn blended urea with 13.8%, ZSB (_Bacillus_ sp.) with 12.5% and the control with 10.5% protein contents. The application of 1.5% bio-activated Zn coated urea caused an increase of 6.4 and 7.8% in protein contents as compared to 1.5% Zn coated and 1.5% Zn blended urea, respectively. The effect of different Zn formulations on the grain Zn concentration of wheat grains is shown in Table 1. The data clearly showed that the application of Zn in different forms significantly (\(p<0.05\)) increased the grain Zn concentration. The use of 1.5% bio-activated Zn coated urea showed 23.60% increase in grain Zn concentration as compared to the control. While the treatments with 1.5% Zn coated urea and 1.5% Zn blended urea resulted in 9.60 and 17.85% increase in grain Zn concentration as compared to the control. The recommended Zn application (ZnSO4) increased grain Zn concentration up to 20.6% as