## 3 Results
### Molecular Identification of Bacterial and Fungal Isolates  
The isolation of genomic DNA was carried out to obtain pure DNA from the bacterial and fungal isolates. The extracted DNA molecules were used as templates for the PCR amplification of the 16S rRNA and ITS genes using the universal primers 27F, 1492R, ITS1F, and ITS4R, respectively, at an annealing temperature of 55 \({}^{\circ}\)C. The amplified products of the bacterial isolates were verified using 1.5% agarose gel stained with ethidium bromide, which revealed a fragment of 1088 bp for the _B. cereus_ strain B-191, 1111 bp for _B. subtilis_, 1099 bp for the _S. marcescens_ strain B-192, and 1106 bp for the _S. marcescens_ strain B-193 when 16S rRNA was used. The _M. circinelloides_ amplification was 600 bp when 18S rRNA was used as the ITS region. The sequence of isolates was submitted to the NCBI. The accession numbers for the bacterial sequence isolates were as follows: MK590996.1--_B. cereus_ strain B-191, MK591144.1--_B. subtilis_ strain B-195, MK591002.1--_S. marcescens_ strain B-192, and MK591014.1--_S. marcescens_ strain B-193 (Table 3). The accession numbers for fungal isolates were as follows: MK559692.1--_M. circinelloides_ isolate (AUMC 10367), identified by internal transcribed spacer 1, partial sequence; the 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and the large subunit ribosomal RNA gene, partial sequence. Sequences from the GenBank nucleotide database were compared to the ones produced. A phylogenetic tree with a 98-100% similarity is used to identify the species. The MEGA 11 program was used to perform the phylogenetic analysis and sequence alignment. The phylogenetic relation between the _B. cereus_ strain B-191, _B. subtilis_ strain B-195, _S. marcescens_ strains B-192 and B-193, and _M. circinelloides_, as well as members of all other _Bacillus_, _Serratia_, and _Mucor_ species are shown in Figures 2-5. The bootstrap values (\(n=1000\)) are shown at the branches. The numbers adjacent to each branch in a tree provide a percentage measure of support for that branch, with 1000 being the maximum support. The 'bootstrapping' approach was performed, and the outcome yielded a high value, indicating that there is significant proof and that the sequences to the side of the branch were grouped exclusively with respect to one another. \begin{table}
\begin{tabular}{c c} \hline
**Characteristic** & **Mean** \\ \hline Circ. & 0.202 \\ Feret & 25,403 \\ \%Area & 100 \\ Feret X & 56.769 \\ Feret Y & 56.538 \\ Feret Angle & 89.393 \\ Min Feret & 11.649 \\ AR & 2.881 \\ Round & 0.413 \\ Solidity & 0.488 \\ \hline \end{tabular}
\end{table} 
Table 2: The above output displays the frequency and cumulative frequency of each characteristic for Mucor circinelloides isolates. Figure 1: SEM images for **(A)**_Bacillus cereus_, **(B)**_Bacillus subtilis_, **(C)**_Serratia marcescens_, and **(D)**_Mucor circinelloides_ detected using SEM at a magnification of 10.00. The scale bar represents 500 \(\upmu\)m.  
Among the nucleotide sequences accessible in the NCBI database, _B. cereus_ (MK590996.1) has a strong resemblance to _B. cereus_ (MK305199.1), _Bacillus_ sp. (MG706123.1), _Bacillus_ sp. (MG430181.1), _B. cereus_ (LC189367.1), and _B. cereus_ (LC189362.1) (Figure 2). _Bacillus subtilis_ (MK591144.1) has a high similarity to _B. subtilis_ (KU206485.1) (99.10%), (KC311340.1) (99.02%), _B. subtilis subsp. subtilis_ (MT114510.1) (99.01%), _B. subtilis_ (MW467894.1) (99.01%), and (MN330082.1) (99.01%) (Figure 3). _marcescens_ (MK591002.1) has a high resemblance to _S. marcescens_ (MK591014.1) and _Serratia_ sp. (OK607431.1) (98.82%), _S. marcescens_ (KR817904.1) (98.73%), _S. marcescens_ (GU212864.1), _Serratia_ sp. (KF891398.1), and _S. marcescens_ (MF669313.1) (Figure 4). _M. circinelloides_ (MK559692.1) shared a high degree of similarity (100%) with _M. circinelloides_ (MK560184.1) and (99.49%) with _M. circinelloides_ (MT603942.1), _M. circinelloides_ (MT603901.1), _M. circinelloides_ (MT603900.1), _M.