## 4 Discussion
In the study, seven days after implantation, the more pronounced inflammatory reactions as well as the presence of multinucleated giant cells in contact with the enclaves of remaining Sealapex and MTA material was noted histologically, while in the case of Bio Aggregate samples, a considerably lower reaction, with the absence of these cells and a reduced inflammatory cell infiltrate could be registered. This aspect indicates a higher biocompatibility of the last material compared to the live subcutaneous tissue, compared to the first two. The inflammatory reaction and the presence of thicker bands of necrotic tissue on contact with implanted materials (thicker at MTA compared to Sealapex and Bioaggregate) in the first seven days may be due to both the different chemical composition of the cements and the stronger basic pH of the MTA: the immediately post mixing pH is 10.2 and rises to 12.5 in 3 h, after which it remains constant, the pH of Sealapex is up to 9.1, and for Bio Aggregate pH-12 after the setting of the material [32; 33; 34]. The presence of large numbers of active osteoblasts and osteoclasts located in specific gaps and the organizing of the new bone tracts 30 days after material implantation, demonstrates an intense activity of bone regeneration and reorganization in the proximity of the implanted material, most evident in MTA and Bio Aggregate [35; 36; 37]. According to some studies from the literature, it may be revealed that MTA, Super EBA, and IRM had similar histopathological results and presented a better performance than ZOE in a puttylike consistency, and only MTA stimulated deposition of neo-formed cementum in direct contact with the retro filling material [38]. Completing the present study, there are a number of other materials that increase the motivation of our study and offer a dimension closer to the ideal that will optimize the results through a modern biomaterial that must meet several requirements, even involving their biological and structural characteristics. The literature points out that graphene has revealed interesting antibacterial and physical peculiarities, but it has also shown limitations. Black phosphorus has structural and biochemical properties that make it ideal for biomedical applications: 2D sheets of black phosphorus are called Black Phosphorene (BP), and it could replace graphene in the coming years. Scientists have taken smart two-dimensional materials (2D materials) into consideration, as the allotropic forms of many such materials have shown distinct characteristics that may be usable in several applications. The interface between cells and biomaterials should be biocompatible and bioactive: 2D materials are tunable on the nanometric-scale, which can be used to improve the connection between such materials and human tissue. An important evaluation regarding the advantages of BP, compared to graphene is related to its lower cytotoxicity, advanced in vivo biodegradability and its tendency to release few nanoparticles in the human body [39]. By means of prosthetic treatments application, our research gives an objective answer of the biocompatibility and functionality that are appreciated as having a response of 88% tolerability and integration, and which are in correlation with compliance with the required conditions and of course the quality of structural components and presentation to clinical treatment, similar to other studies in the literature [40; 41; 42; 43; 44; 45; 46; 47; 48]. Another issue that must be accounted for is the biocompatibility and the integration capabilities of materials used in the context of systemic diseases, such as tumors, fractures, vitamin deficiencies, liver pathology, and autoimmune diseases [49; 50; 51; 52; 53; 54]. Although in the majority of cases, such issues do not critically alter the treatment course, still, precautions must be taken in order to ensure the best possible outcome [55; 56].