# On the Use of Image Analysis for Hematocrit Evaluation in Dried Blood Spots
###### Abstract
Available online: [[https://www.ema.europa.eu/en/ich-m10-bioanalytical-method-validation-scientific-guideline](https://www.ema.europa.eu/en/ich-m10-bioanalytical-method-validation-scientific-guideline)]([https://www.ema.europa.eu/en/ich-m10-bioanalytical-method-validation-scientific-guideline](https://www.ema.europa.eu/en/ich-m10-bioanalytical-method-validation-scientific-guideline)) (accessed on 9 September 2024). * [PERSON] et al. (2019) [PERSON] [PERSON]; [PERSON] [PERSON]; [PERSON] [PERSON]; [PERSON] [PERSON] Hematocrit effect on dried blood spots in adults: A computational study and theoretical considerations. Scand. _J. Clin. Lab. Investig._**2019**, _79_, 325-333. [CrossRef]
* [PERSON] et al. (2019) [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON]; et al. Official International Association for Therapeutic Drug Monitoring and Clinical Toxicology Guideline: Development and validation of Dried Blood Spot-based methods for Therapeutic Drug Monitoring. _Ther. Drug Monit._**2019**, _41_, 409-430. [CrossRef]
* [PERSON] et al. (2014) [PERSON]; [PERSON]; [PERSON]; [PERSON] Current strategies for coping with the hematocrit problem in dried blood spot analysis. _Bioanalysis_**2014**, \(6\), 1871-1874. [CrossRef]
* [PERSON] et al. (2019) [PERSON]; [PERSON]; [PERSON] Is the hematocrit still an issue in quantitative dried blood spot analysis? _J. Pharm. Biomed. Anal._**2019**, _163_, 188-196. [CrossRef]
* [PERSON] et al. (2015) [PERSON]; [PERSON]; [PERSON] Does volumetric absorptive microsampling eliminate the hematocrit bias for caffeine and paraxanthine in dried blood samples? A comparative study. _Anal. Chim. Acta_**2015**, _881_, 65-73. [CrossRef]
* [PERSON] et al. (2011) [PERSON]; [PERSON] [PERSON]; [PERSON]; [PERSON] Perforated dried blood spots: A novel format for accurate microsampling. _Bioanalysis_**2011**, \(3\), 2321-2333. [CrossRef]
* [PERSON] et al. (2015) [PERSON]; [PERSON]; [PERSON] [PERSON]; [PERSON] [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON] New microfluidic-based sampling procedure for overcoming the hematocrit problem associated with dried blood spot analysis. _Anal. Chem._**2015**, _87_, 2068-2071. [CrossRef]
* [PERSON] and [PERSON] (2016) [PERSON] [PERSON]; [PERSON] [PERSON] Hematocrit-independent quantitation of stimulants in Dried Blood Spots: Pipet versus microfluidic-based volumetric sampling coupled with automated Flow-Through Desorption and Online Solid Phase Extraction-LC-MS/MS Bioanalysis. _Anal. Chem._**2016**, _88_, 6789-6796. [CrossRef]
* [PERSON] et al. (2015) [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON] Investigation of different approaches to incorporating internal standard in DBS quantitative bioanalytical workflows and their effect on nullifying hematocrit-based assay bias. _Anal. Chem._**2015**, _87_, 4996-5003. [CrossRef] [PubMed]
* [PERSON] et al. (2022) [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON] Dried Plasma Spot Based LC-MS/MS Method for Monitoring of Meropenem in the Blood of Treated Patients. _Molecules_**2022**, _27_, 1991. [CrossRef] [PubMed]
* [PERSON] et al. (2020) [PERSON]; [PERSON]; [PERSON] [PERSON]; [PERSON]; [PERSON]; [PERSON] [PERSON] The potential of neurofilaments analysis using dry-blood and plasma spots. _Sci. Rep._**2020**, _10_, 97. [CrossRef]
* [PERSON] et al. (2018) [PERSON]; [PERSON]; [PERSON]; [PERSON] Development of a hematocrit-insensitive device to collect accurate volumes of dried blood spots without specialized skills for measuring clozapine and its metabolites as model analytes. _J. Chromatog. B_**2018**, _1087-1088_, 70-79. [CrossRef]
* [PERSON] et al. (2013) [PERSON]; [PERSON]; [PERSON]; [PERSON] Prediction of the hematocrit of dried blood spots via potassium measurement on a routine clinical chemistry analyser. _Anal. Chem._**2013**, _85_, 404-410. [CrossRef]
* [PERSON] et al. (2014) [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON] Potassium-based algorithm allows correction for the hematocrit bias in quantitative analysis of caffeine and its major metabolite in dried blood spots.