## 3 Results
### Microbiological Counts  
The microbiological counts in the different culture media and at different temperatures are shown in Table 2. It was observed that the total number of total aerobic mesophiles increased significantly (\(p<0.05\)) with time, more at 25 \({}^{\circ}\)C than at 4 \({}^{\circ}\)C, both in the controls and in all the treated samples. For presumptive _Enterobacteriaceae_ at 25 \({}^{\circ}\)C, the highest reductions of viable cell counts were obtained for treatment A, reducing the number of colonies below the detection limit on day 7. Significant differences were also observed in treatment E compared to untreated controls because the viable cell concentrations of presumptive _Enterobacteriaceae_ did not increase during incubation, but rather remained at values close to time 0. Treatment P (inoculation with the _Paenibacillus_ strain) also seemed to delay the proliferation of presumptive _Enterobacteriaceae_ compared to controls at the end of storage. At 4 \({}^{\circ}\)C, presumptive _Enterobacteriaceae_ were significantly lower at day 7 for all treatments, but the reduction of viable counts by treatment A was much lower compared to 25 \({}^{\circ}\)C. In the _Bacillus cereus_ agar medium, significantly lower counts (\(p<0.05\)) were observed mainly in treatments E and A at 25 \({}^{\circ}\)C with respect to the control. At 4 \({}^{\circ}\)C, viable cell counts decreased during incubation for all the samples, with no significant differences between controls and treated samples. The counts obtained on MRS agar (presumptive lactic acid bacteria) increased with time more at 25 \({}^{\circ}\)C than at 4 \({}^{\circ}\)C. Only treatments E (days 1 and 2) and P (day 2) showed significant differences with controls, at 25 \({}^{\circ}\)C.  
\begin{table}
\begin{tabular}{l c c c c c c} \hline  & & & & & **Test Strains** & \\ \hline \multirow{2}{*}{**Eluate**} & _S. aureus_ & _E. faecalis_ & _S. enterica_ & _E. coli_ & _L. innocua_ & _B. cereus_ \\  & **CECT 976** & **S-47** & **CECT 3197** & **E19** & **CECT 910** & **LWL1** \\ \hline NaCl (E) & 13 & 0 & 13 & 14 & 0 & 12 \\ Acn (A) & 16 & 0 & 9 & 9 & 0 & 17 \\ \hline \end{tabular}
\end{table}
Table 1: Antimicrobial activities of eluates obtained by cation exchange chromatography (E) and reversed-phase chromatography (A). Values indicate growth inhibition zone (in mm).  
\begin{table}
\begin{tabular}{c c c c c} \hline  & **T0** & **T1** & **T2** & **T7** \\ \hline _Enterobacteriaceae_ 25 \({}^{\circ}\)C & & & & \\ \hline C & 5.37 \(\pm\) 0.02 & 4.70 \(\pm\) 0.004 & 4.48 \({}^{\mathrm{b}}\)\(\pm\) 0.00 & 7.48 \({}^{\mathrm{a}}\)\(\pm\) 0.00 \\ \hline P & 5.29 \(\pm\) 0.03 & 5.84 \({}^{\mathrm{a}}\)\(\pm\) 0.03 & 4.48 \({}^{\mathrm{b}}\)\(\pm\) 0.00 & 6.59 \({}^{\mathrm{a}}\)\(\pm\) 0.01 \\ \hline E & 4.11 \({}^{\mathrm{c}}\)\(\pm\) 0.01 & 4.95 \({}^{\mathrm{a}}\)\(\pm\) 0.05 & 4.48 \({}^{\mathrm{a}}\)\(\pm\) 0.00 & 4.48 \({}^{\mathrm{a}}\)\(\pm\) 0.00 \\ \hline A & 5.25 \(\pm\) 0.04 & 1.00 \({}^{\mathrm{h}c}\)\(\pm\) 0.00 & 1.15 \({}^{\mathrm{h}c}\)\(\pm\) 0.15 & \(<\)1.00 \\ \hline _Bacillus cereus_ agar 25 \({}^{\circ}\)C & & & & \\ \hline C & 3.60 \(\pm\) 0.03 & 5.31 \({}^{\mathrm{a}}\)\(\pm\) 0.01 & 6.65 \({}^{\mathrm{a}}\)\(\pm\) 0.02 & 4.48 \({}^{\mathrm{a}}\)\(\pm\) 0.00 \\ \hline P & 5.05 \(\pm\) 0.01 & 5.27 \(\pm\) 0.01 & 5.05 \({}^{\mathrm{c}}\)\(\pm\) 0.03 & 4.48 \({}^{\mathrm{b}}\)\(\pm