## 1 Introduction  
Oral health is important in daily life since it reflects general physical health [1; 2; 3]. Oral disease affects teeth and causes gut microbial dysbiosis, cardiovascular illness, urinary tract infections, arthritis, cystic fibrosis, and brain disease [4; 5; 6; 7; 8; 9]. Even though brushing and flossing can be effective methods of removing plaque, toothbrushes can operate as reservoirs for harmful germs that later inoculate the oral cavity, highlighting the need to properly sanitize and store personal dental hygiene devices [10; 11; 12; 13]. The mouth rinsing custom has been a part of human culture for more than 200 decades. [PERSON] et al. reported that natural mouthwashes could serve as substitutes to chemical agents such as chlorhexidine, providing effective caries control and safe long-term use [14]. Mouth rinses containing dextranase were superior to 0.12% chlorhexidine mouthwash in preventing plaque accumulation caused by _Lipomyces starkeyi_ KSM 22 and _Catenovulum agariorans_ MNH15 in human experimental gingivitis [15; 16]. Likewise, nisin-containing mouth rinse was proven to be a strong plaque inhibitor. Nisin inhibits the growth of oral bacteria and the development of biofilms by preventing cell attachment and proliferation. Nisin either binds to nonspecific lipids on cytoplasmic membranes or to specific Lipid II, a crucial component of the cell membranes of Gram-positive and Gram-negative bacteria such as _Prevotella intermedia_ (_P. intermedia_), _Porphyromonas gingivalis_ (_P. gingivalis_), and _Aggregatibacter actinomycetemcomitans_ (_A. actinomycetemcomitans_). The antibacterial effect of ethanolic extract of licorice root, Triphala, and garlic on _Streptococcus mutans_ (_S. mutans_) was less effective when compared with chlorhexidine mouthwash [17; 18]. The collective genome of microbes in the oral cavity is the oral microbiome linked to taste thresholds such as sweet, sour, salty, and bitter, used to maintain its microbial membership and permanence in the oral cavity [19; 20]. Additionally, gingival-related microbiota such as _S. mutans_, _Streptococcus goronii_, _Streptococcusalis_, _Lacticaseibacillus casei_, and _P. gingivalis_ have been discovered to have a critical role in governing human health and illness. Additionally, when it grows abundantly, it substantially supports the growth of Gram-negative bacteria such as _P. gingivalis_, _Aggregatibacter actinomycetemcomitans_ (_A. actinomycetemcomitans_), _P. intermedia_, and _Tannerella forysthia_ (_T. forysthia_), resulting in periodontal organ inflammation [21; 22]. The most common oral pathogens, such as _S. mutans_, _P. gingivalis_, _Staphylococcus_ species, and _Lactobacillus_ species, are present in dental plaque and cavities [23; 24; 25]. The _Lacticobacillus_ fermions sugar and produces a large amount of lactic acid, which can easily cause caries [26]. Oral bacteria reach the digestive tract rapidly through the airway, generating an imbalance in the intestinal microflora and harming the digestive system, particularly pathogenic periodontitis bacteria [27; 28; 29; 30]. Likewise, _Fusobacterium nucleatum_ colonizes and governs the gastrointestinal tract via the blood flow system, as outlined in a prior study on colorectal cancer [31]. Additionally, _P. gingivalis_ can target the complement C5a receptor 1 (C5 aR1) and the Toll-like receptor 2 (TLR2) [32]. _P. gingivalis_ and _Helicobacter pylori_ can activate the PI3K signaling pathway, inhibiting internalization and increasing inflammation, targeting these two receptors and leading to pancreatic cancer [33; 34]. The prevalence of _Neisseria_ and _Haemophilus_ differs between hunter-gatherers and westerners, with rural farmers falling somewhere in the middle. Some oral pathogens have been discovered among hunter-gatherers, indicating that consuming too much meat increases the risk of dental degenerative diseases [35]. It is necessary to elucidate the complete microbiome within the human mouth.