## Results and Discussion
### Genome-Centric Evaluation of Strain SRS-W-1-2016
strain SRS-W-1-2016 with the first (outermost) and fourth rings depicting clusters of orthologous groups of proteins (COG) categories of protein coding genes on the forward and reverse strands, respectively. The second and third rings show the locations of protein coding, tRNA, and rRNA genes on the forward and reverse strands, respectively. The black plot depicts GC content with the peaks extending towards the outside of the circle representing GC content above the genome average, whereas those extending towards the center mark segments with GC content lower than the genome average. The innermost plot depicts GC skew. Both base composition plots were generated using a sliding window of 50,000 nt. _Arthrobacter_ enhance the stability of nucleic acids, proteins, and lipids, allowing bacteria to survive in extreme conditions [27], such as the SRS co-contaminated soils. Some species of _Arthrobacter_ such as _Arthrobacter crystallopoietes_[28] and _Arthrobacter chlorophenolicus_[29] have been utilized for the bioremediation of chromium and 4-chlorophenol in contaminated soils. Generally, _Arthrobacter_ is known to grow on complex aromatic substrates such as hydroxybenzoate, pyridine, and picoline [30], which is a unique biodegradable ability of this group of soil bacteria. In fact, as stated earlier in this study, _Arthrobacter_ spp., have been shown to successfully colonize habitats characterized by extreme environmental stresses, including nuclear waste plume heavy metal contaminated sites, as well as uraniferous niches, such as the U-impacted DOE Hanford site [15], which points to the genomic-mechanisms possessed by _Arthrobacter_ spp., for the colonization of metal-rich ecosystems. To further analyze the genome of strain SRS-1-W-2016, a KEGG -based hierarchical functional gene clustering analysis was performed (Table 1), which revealed the presence of a total of 1124 KEGG-associating genes; the top five categories belonged to carbohydrate metabolism (284 genes; 25.27%); amino acid metabolism (256 genes; 22.78%); energy metabolism (135 genes; 12.01%); vitamin and cofactor metabolism (128 genes; 11.39%); and membrane transport (105 genes; 9.34%), respectively. Additionally, a total of 2844 protein coding genes associated with COGs in strain SRS-W-1-2016 were found with further classification into 25 categories, with the most abundant COG subsystems as follows: carbohydrate transport and metabolism; and amino acid transport and metabolism. The transcription of many of these features suggests the propensity of this strain to engage in high metabolic activity in its native contaminated environment. These analyses provide a strong foundation for the presence of several genome-enabled metabolic and catabolic processes in _Arthrobacter_ spp. strain SRS-W-1-2016 which likely play a significant role in its successful colonization and survival within the SRS co-contaminated soil habitat. \begin{table}
\begin{tabular}{c c} \hline \hline
**KEGG Category** & **Percentage (\%)** \\ \hline Global and Overview Maps & 63.08 \\ Carbohydrate Metabolism & 25.27 \\ Amino Acid Metabolism & 22.78 \\ Energy Metabolism & 12.01 \\ Metabolism of Cofactors and Vitamins & 11.39 \\ Membrane Transport & 9.34 \\ Nucleotide Metabolism & 8.72 \\ Lipid Metabolism & 7.56 \\ Translation & 7.21 \\ Xenobiotics Biodegradation and Metabolism & 6.32 \\ Signal Transduction & 5.87 \\ Metabolism of other Amino Acids & 4.8 \\ Replication and Repair & 4.54 \\ Folding, Sorting and Degradation & 4.18 \\ Metabolism of Terpenoids and Polyketides & 3.91 \\ Biosynthesis of other Secondary Metabolites & 3.2 \\ Glycan Biosynthesis and Metabolism & 2.85 \\ Cell Motility & 2.67 \\ Cell Growth and Death & 1.42 \\ Drug Resistance & 1.33 \\ Transport and Catabolism & 0.98 \\ Transcription & 0.44 \\ Environmental Adaptation & 0.18 \\ Excretery System & 0.18 \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Functional traits connected with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways identified from the whole genome sequence of _Arthrobacter_ spp. strain SRS-W-1-2016.