_Applied_  
_sciences_  
_Article_  
**Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light**  
**[PERSON] 1,2\({}^{\text{(c)}}\), [PERSON] 1,3, [PERSON] 1,4,5\({}^{\text{(c)}}\), [PERSON] 1,4,5, [PERSON] 2,6\({}^{\text{(c)}}\), [PERSON] 4,5,7 and [PERSON] 4,*\({}^{\text{(c)}}\)**  
1 Department of Physiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic; [EMAIL_ADDRESS] (M.F.); [EMAIL_ADDRESS] (T.V.); [EMAIL_ADDRESS] (J.G.); [EMAIL_ADDRESS] (M.R.)  
2 Department of Biomedical Sciences, Faculty of Health and Society, Malmo University, 214 28 Malmo, Sweden; [EMAIL_ADDRESS]  
3 Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, 616 00 Brno, Czech Republic  
4 Department of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic; [EMAIL_ADDRESS]  
5 Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic  
6 Biofilms Research Center for Biointerfaces, Malmo University, 214 28 Malmo, Sweden  
7 BIOCEV, First Faculty of Medicine, Charles University, Prumyslova 595, 252 50 Vestec, Czech Republic  
* Correspondence: [EMAIL_ADDRESS]  
Received: 12 March 2020; Accepted: 4 April 2020; Published: 9 April 2020  
**Featured Application: Quantitative phase imaging (QPI) and holographic microscopy (HM) are suitable methods for long-term and label-free imaging of cells and for the complex evaluation of cellular morphological and dynamical parameters under different treatments. Our results suggest that HM within advanced image analysis is capable of correctly distinguishing between subtle changes in cell motility, cell dry mass, cell density, and cell death induced by blue light. As quantitative phase dynamics of the cell population is typical for specific processes in cells, it may be useful for viability tests and medical drugs evaluation. In particular, mapping of the quantitative phase changes during the time seems to be very promising in the determination of various morphological phenomena related to cell death, because the responsible cell death molecular machinery typically changes the distribution of the cell mass.**  
**Abstract: Increased exposition to blue light may induce many changes in cell behavior and significantly affect the critical characteristics of cells. Here we show that multimodal holographic microscopy (MHM) within advanced image analysis is capable of correctly distinguishing between changes in cell motility, cell dry mass, cell density, and cell death induced by blue light. We focused on the effect of blue light with a wavelength of 485 nm on morphological and dynamical parameters of four cell lines, malignant PC-3, A2780, G361 cell lines, and the benign PNT1A cell line. We used MHM with blue light doses 24 mJ/cm\({}^{2}\), 208 mJ/cm\({}^{2}\) and two kinds of expositions (500 and 1000 ms) to acquire real-time quantitative phase information about cellular parameters. It has been shown that specific doses of the blue light significantly influence cell motility, cell dry mass and cell density. These changes were often specific for the malignant status of tested cells. Blue light dose 208 mJ/cm\({}^{2}\)\(\times\) 1000 ms affected malignant cell motility but did not change the motility of benign cell line PNT1A. This light dose also significantly decreased proliferation activity in all tested cell lines but was not so deleterious for benign cell line PNT1A as for malignant cells. Light dose 208 mJ/cm\({}^{2}\)\(\times\) 1000 ms oppositely affected cell mass in A2780 and PC-3 cells and induced different types of cell death in A2780 and G361 cell lines. Cells obtained the least damage on lower doses of light with shorter time of exposition.**  
**Keywords:** holographic microscopy; quantitative phase imaging; blue light; cell mass; cell motility; cell death +
Footnote †: journal: Journal of chemical and biochemical Research