## 1 Introduction  
Bio-asphalt, as a new green environmental protection material, has a similar chemical composition to petroleum asphalt [1, 2, 3]. It can also save costs, reduce carbon emissions, and has good environmental benefits. Research has shown that the pyrolysis of biomass oil is not complete in the preparation process, and that it is susceptible to undergoing polymerization and esterification during storage, which increases its own viscosity [4, 5]. Moreover, the polar and phenolic substances in bio-oil also can provide good adhesion performance by mixing it into petroleum asphalt. However, if the adhesion between asphalt and the aggregates is reduced, then pavement loosening, potholes, and other distresses will occur. This affects road performance and service life under the long-term influence of the external environment (heat, light, oxygen, and water) [6; 7]. Currently, various methods have been used to evaluate the adhesion of asphalt-aggregate interfaces. [PERSON] et al. [8] analyzed the influence of asphalt-aggregate adhesion on moisture resistance and the fatigue life of four different asphalt mixtures through digital image processing, a modified Lottman test, and the tensile compression test. As a result, it was found that the type of aggregate has a significant relationship with the moisture-induced damage resistance between asphalt and aggregates. [PERSON] et al. [9] used photoelectric colorimetry to compare the adhesion of three asphalt binders, SK-90, SBS-MA, and HCRA-60, to limestone and basalt. Results showed that the asphalt binders still exhibited excellent adhesive properties after mixing a large amount of rubber powder and a small amount of waste oil into the base asphalt. [PERSON] et al. [10] used surface free energy theory to study the contribution of direct coal liquefaction residue (DCLR) to asphalt-aggregate adhesion. It was demonstrated that, as the dosage of DCLR increased, the surface free energy and adhesion property of asphalt increased before and after aging, thus exhibiting good adhesivity. [PERSON] et al. [11] evaluated the factors affecting the asphalt-aggregate bonding performance by improving the pull-off test. And it was found that the fractions of saturates, aromatics, resins, and asphaltenes (SARA) had the greatest influence on the bonding strength between asphalt and aggregates. Furthermore, asphaltene and resin can enhance the adhesion of the asphalt to the aggregate. [PERSON] et al. [12] evaluated the performance of bio-modified, rubberized asphalt under the conditions of thermal aging, UV aging, and moisture with the moisture-induced shear-thinning index (MISTI) and the contact angle moisture susceptibility test (CAMSI). They found that bio-oil could improve the resistance of asphalt to cohesion damage by three times, and that bio-oil could also retard the reaction of free radicals with asphalt. Meanwhile, the aging and type of bio-asphalt also affects the adhesion performance of bio-asphalt-aggregate. [PERSON] et al. [13] found that the chemical composition of bio-asphalt made from different plant-originated bio-oil directly affects its sensitivity to aging. As a result, the adhesion of asphalt-aggregate under moisture damage conditions is significantly decreased. [PERSON] et al. [14] prepared bio-asphalt by adding timber-originated biochar to petroleum asphalt and tested its aging resistance. The results demonstrated that biochar could reduce the effect of aging on the performance of asphalt binders. [PERSON] et al. [15] found that the recycled asphalt binder containing bio-additives had a lower aging sensitivity, better mechanical properties, and less susceptibility to cracking than original asphalt. At the same time, [PERSON] et al. [16] also observed that the high content of oxygen and light components in bio-oil also make bio-asphalt generally prone to aging. Therefore, the aging and moisture damage resistance of bio-asphalt is particularly important for the study of adhesivity in bio-asphalt-aggregate.