## 3 Results and Discussion
### Selected Vitamin Content  
Our results revealed that the seven edible mushrooms contained moderate amounts of ascorbic acid (vitamin C) (Figure 4a). Nevertheless, _R. brevipes_ showed the highest levels (>80 mg 100 g\({}^{-1}\) DW), whereas the lowest levels were found in _L. indigo_ and _C. nuda_ (<10 mg 100 g\({}^{-1}\) DW). These results suggest that the studied mushrooms are an overlooked source of vitamin C and possess higher levels than those reported in common edible sources such as _Agaricus bisporus_, _Lentinus sedodes_, and _Pleurotus ostreatus_[21]. The content of thiamine (vitamin B\({}_{1}\)) was abundant in _C. subclavipes_ (>200 ug 100 g\({}^{-1}\) DW), _L. indigo_ (>100 ug 100 g\({}^{-1}\) DW), and _C. nuda_ (>100 ug 100 g\({}^{-1}\) DW) (Figure 4b). The other species contained less than 90 ug 100 g\({}^{-1}\) DW thiamine. Interestingly, these levels surpassed those described for _A. bisporus_, _L. edodes_, and _P. ostreatus_[21]. The levels of riboflavin were remarkably higher in _L. indigo_ (>300 ug 100 g\({}^{-1}\) DW) and _C. nuda_ (>400 ug 100 g\({}^{-1}\) DW) in comparison with those observed in the other five species, which oscillated from 30 to 100 ug 100 g\({}^{-1}\) DW (Figure 4c). These levels were comparable to those already reported for _A. bisporus_, _L. edodes_, and _P. ostreatus_, which are considered common edible mushrooms consumed worldwide [21]. The content of bioactive forms of vitamin B\({}_{6}\) in edible mushrooms is still a big issue to be addressed, since little information is available in the current scientific literature. However, the levels of this vitamin in _Flumulina velitopes_, _Grifola frondosa_, _A. bisporus_, and _P. ostreatus_ oscillate from 20 to 100 ug 100 g\({}^{-1}\) DW [22]. Our results suggest that _C. nuda_ contains almost fourfold more levels of vitamin B\({}_{6}\) (>400 ug 100 g\({}^{-1}\) DW) than the latter selected mushrooms frequently eaten in North America (Figure 4d). _L. indigo_ and _C. subclavipes_ contained -200 ug 100 g\({}^{-1}\) DW vitamin B\({}_{6}\), whereas the other four species showed levels below 100 ug 100 g\({}^{-1}\) DW vitamin B\({}_{6}\) (Figure 4d). The folate content was higher in _R. brevipes_ (-100 ug 100 g\({}^{-1}\) DW) than in the other mushrooms studied (Figure 4e). _L. indigo_ and _C. nuda_ showed levels over 50 ug 100 g\({}^{-1}\) DW folate, whereas the other four species presented below 50 ug 100 g\({}^{-1}\) DW folate. The content of this vitamin was higher than that reported for _F. velitopes_, _G. frondosa_, and _A. bisporus_, but lower than that described for _L. edodes_, and _P. ostreatus_[21,22].  
Figure 4: Contents of vitamin C (**a**), thiamine (**b**), riboflavin (**c**), vitamin B\({}_{6}\) (**d**), and folic acid (**e**) in seven edible mushrooms consumed in the northeastern highlands of Puebla Mexico. 1, _Lactarius_.  
indigo. 2_, _Clitocybe nuda. 3_, _Clitocybe subclavipes. 4_, _Russula delica. 5_, _Russula brevipes. 6_, _Clitocybe squammusa. 7_, _Amanita jacksonii_. Different letters indicate means (_n_ = 10) with statistically significant differences by ANOVA-Tukey (_p_ < 0.05). Raw data are presented in Table S2. The concentrations are presented in dry weight.