## 3 Results
### Halimione portulacoides Fatty Acids Profile
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multicolumn{6}{c}{**Aquaponics**} \\ \cline{2-7}
**Fatty Acids** & \multicolumn{3}{c}{**Edible Plant Part**} & \multicolumn{3}{c}{**Root**} \\ \cline{2-7}  & **Spring** & **Summer** & **Autumn** & **Spring** & **Summer** & **Autumn** \\ \hline
**18:1**\(n\)-9** & 0.78 \(\pm\) 0.03 & 1.10 \(\pm\) 0.04 & 0.81 \(\pm\) 0.01 & 0.34 \(\pm\) 0.02 & 0.45 \(\pm\) 0.02 & 0.79 \(\pm\) 0.03 \\ \hline
**18:1**\(n\)-7** & 0.03 \(\pm\) 0.00 & 0.06 \(\pm\) 0.00 & 0.06 \(\pm\) 0.00 & 0.08 \(\pm\) 0.01 & 0.50 \(\pm\) 0.03 & 0.34 \(\pm\) 0.02 \\ \hline
**20:1**\(n\)-7** & 0.05 \(\pm\) 0.00 & 0.05 \(\pm\) 0.00 & 0.05 \(\pm\) 0.00 & ND & ND & ND \\ \hline \(\sum\)**MUFA** & 1.03 \(\pm\) 0.04 & 1.41 \(\pm\) 0.05 & 1.04 \(\pm\) 0.01 & 0.51 \(\pm\) 0.04 & 2.24 \(\pm\) 0.16 & 1.90 \(\pm\) 0.07 \\ \hline
**16:3**\(n\)-3** & 0.40 \(\pm\) 0.02 & 0.25 \(\pm\) 0.01 & 0.14 \(\pm\) 0.00 & ND & ND & ND \\ \hline
**18:2**\(n\)-6** & 2.41 \(\pm\) 0.13 & 2.46 \(\pm\) 0.12 & 2.25 \(\pm\) 0.04 & 3.18 \(\pm\) 0.20 & 1.91 \(\pm\) 0.09 & 3.42 \(\pm\) 0.10 \\ \hline
**18:3**\(n\)-3** & 5.29 \(\pm\) 0.29 & 7.11 \(\pm\) 0.33 & 5.10 \(\pm\) 0.08 & 0.45 \(\pm\) 0.04 & 0.25 \(\pm\) 0.01 & 0.71 \(\pm\) 0.02 \\ \hline
**20:2**\(n\)-6** & 0.13 \(\pm\) 0.00 & 0.10 \(\pm\) 0.00 & 0.10 \(\pm\) 0.00 & ND & ND & ND \\ \hline \(\sum\)**PUFA** & 8.23 \(\pm\) 0.44 & 9.92 \(\pm\) 0.46 & 7.59 \(\pm\) 0.12 & 3.63 \(\pm\) 0.24 & 2.16 \(\pm\) 0.10 & 4.13 \(\pm\) 0.12 \\ \hline
**PUFA/SFA** & 3.30 & 3.01 & 2.67 & 1.73 & 1.10 & 1.42 \\ \hline \hline \end{tabular}
\end{table} 
Table 1: _Cont._  
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multicolumn{6}{c}{**Wild**} \\ \cline{2-7}
**Fatty Acids** & \multicolumn{3}{c}{**Edible Plant Part**} & \multicolumn{3}{c}{**Root**} \\ \cline{2-7}  & **Spring** & **Summer** & **Autumn** & **Spring** & **Summer** & **Autumn** \\ \hline
**16:0** & 2.94 \(\pm\) 0.01 & 0.97 \(\pm\) 0.02 & 1.52 \(\pm\) 0.08 & 0.71 \(\pm\) 0.00 & 0.69 \(\pm\) 0.10 & 0.46 \(\pm\) 0.01 \\ \hline
**18:0** & 0.21 \(\pm\) 0.01 & 0  
the first two axes of the PCO performed, with 91.0% of total variance being explained for _H. portulacoides_, with PCO1 axis explaining 60.2% of total variance and clearly separating cultured from wild specimens. Significant differences were also recorded in the FA profiles during spring, summer, and autumn (Pseudo-F2,17 = 210.3; \(p\) = 0.0001). Regarding root part, the PERMANOVA test revealed that there were significant differences among the FA profiles for cultured and wild _H. portulacoides_ (Pseudo-F1,17 = 530.31; \(p\) = 0.001). The first two axis of the PCO analysis (Figure 3) explained 90.6% of total variance, with PCO1 axis explaining 79.3% of total variance and clearly separating cultured in aququonics from wild specimens. Significant differences were also recorded in the FA profiles during spring, summer, and autumn (Pseudo-F2,17 = 44.354; \(p\) = 0.001). All species, regardless of being wild or cultured in aququonics, displayed a PUFA/SFA ratio > 0.45, the threshold value indicative of good nutritional quality see Tables 1 and 2.