## 3 Results
### [ATP]/[ADP] Ratio, ATPase Activity, and cAMP Concentration Determinations

Table 3: Relative transcription levels determined using RT-qPCR for several groups of genes in strains PB12 and PB12 AGD\({}^{+}\). \begin{table}
\begin{tabular}{l l c c} \hline \hline
**Pathway, Group of Genes** & **Encoded Protein 1** & \multicolumn{2}{c}{**Relative Transcription**} \\
**or Cellular Process** & & **Levels as 2\({}^{-\Delta\Delta\textbf{Ct}}\)** \\ \hline  & & **PB12** & **PB12 AGD\({}^{+}\)** \\ \hline _atpG_ & Subunit \(\gamma\) & 0.79 \(\pm\) 0.17 & 8.48 \(\pm\) 1.60 \\ _atpD_ & Subunit \(\beta\) & 0.66 \(\pm\) 0.11 & 10.4 \(\pm\) 1.89 \\ \hline Glucose transport & & & \\ \hline _galP \({}^{*}\)_ & Galactose:H\({}^{+}\) symporter & 6.05 \(\pm\) 0.63 & 3.28 \(\pm\) 0.33 \\ _ompF \({}^{*}\)_ & Outer membrane porin F & 0.54 \(\pm\) 0.08 & 0.44 \(\pm\) 0.09 \\ _ptsH \({}^{*}\)_ & Phosphocarrier protein Hpr & 0 & 0 \\ _ptsG \({}^{*}\)_ & Glucose-specific PTS enzyme IIBC & 1.53 \(\pm\) 0.22 & 0.89 \(\pm\) 0.23 \\  & component & & \\ \hline Acetate transport & & & \\ \hline _actP (yjcG) \({}^{*}\)_ & Acetate/glycolate:cation & & \\  & symporter & & \\ \hline Glycolysis and gluconeogenesis & & & \\ \hline _Eno_ & Enolase & 0.66 \(\pm\) 0.07 & 0.33 \(\pm\) 0.03 \\ _fluA \({}^{*}\)_ & Fructose-bisphosphate aldolase & 0.64 \(\pm\) 0.13 & 0.21 \(\pm\) 0.09 \\  & class II & & \\ _fluB_ & Fructose-bisphosphate aldolase & 4.41 \(\pm\) 0.94 & 2.64 \(\pm\) 0.59 \\ _fbp_ & Fructose-1,6-bisphosphatase 1 & 2.51 \(\pm\) 0.00 & 1.60 \(\pm\) 0.02 \\ _gapA \({}^{*}\)_ & Clyceraldehyde-3-phosphate & 1.04 \(\pm\) 0.16 & 0.47 \(\pm\) 0.04 \\ _glk_ & Glucokinase & 2.98 \(\pm\) 0.31 & 1.45 \(\pm\) 0.30 \\  & 2,3-bisphosphoglycercate- & & \\ _gpmA_ & dependent phosphoglycerate & 2.36 \(\pm\) 0.67 & 1.09 \(\pm\) 0.06 \\  & mutase & & \\ _gpmB_ & Putative phosphatase & 1.59 \(\pm\) 0.05 & 1.42 \(\pm\) 0.05 \\ _pkA_ & 6-phosphofructokinase 1 & 0.96 \(\pm\) 0.03 & 0.48 \(\pm\) 0.04 \\ _psi_ & Glucose-6-phosphate isomerase & 4.30 \(\pm\) 0.35 & 2.40 \(\pm\) 0.25 \\ _pgk \({}^{*}\)_ & Phosphoglycerate kinase & 0.77 \(\pm\) 0.04 & 0.55 \(\pm\) 0.02 \\ _ppc_ & Phosphoenolpyruvate carboxylase & 1.53 \(\pm\) 0.02 & 1.63 \(\pm\) 0.11 \\ _pykA_ & Pyruvate kinase 2 & 2.01 \(\pm\) 0.24 & 1.09 \(\pm\) 0.06 \\ _tpiA_ & Triose-phosphate isomerase & 1.45 \(\pm\) 0.01 & 0.68 \(\pm\) 0.02 \\ \hline Pentose phosphate pathway & & & \\ \hline _ada_ & KHG/KDPG aldolase & 2.07 \(\pm\) 0.18 & 1.76 \(\pm\) 0.09 \\ _gnd_ & 6-Phosphogluconate & 1.29 \(\pm\) 0.49 & 0.63 \(\pm\) 0.10 \\ _rpiA_ & Ribose-5-phosphate isomerase A & 1.63 \(\pm\) 0.40 & 1.39 \(\pm\) 0.06 \\ _rpiB_ & Ribose-5-phosphate isomerase B & 2.52 \(\pm\) 0.49 & 2.29 \(\pm\) 0.55 \\ _talA_ & Transaldolase A & 3.53 \(\pm\) 0.37 & 1.80 \(\pm\) 0.32 \\ _talB_ & Transaldolase B & 1.49 \(\pm\) 0.05 & 0.76 \(\pm\) 0.25 \\ _tktB_ & Transketolase 2 & 3.39 \(\pm\) 0.06 & 2.58 \(\pm\) 0.57 \\  & NADPP\({}^{+}\)-dependent & & \\ _zwf_ & glucose-6-phosphate & 1.96 \(\pm\) 0.24 & 1.25 \(\pm\) 0.08 \\  & dehydrogenase & & \\ \hline TCA cycle and the glyoxylate shunt & & & \\ \hline _acCB \({}^{*}\)_ & Malate synthase A & 1.56 \(\pm\) 0.00 & 0.78 \(\pm\) 0.11 \\ _acCA \({}^{*}\)_ & Isocitrate lyase & 3.03 \(\pm\) 0.32 & 1.17 \(\pm\) 0.03 \\ _acCK \({}^{*}\)_ & Isocitrate dehydrogenase kinase & 3.08 \(\pm\) 0.20 & 1.61 \(\pm\) 0.04 \\ _acCE \({}^{*}\)_ & Pyruvate dehydrogenase & 1.36 \(\pm\) 0.25 & 0.78 \(\pm\) 0.11 \\ _acCF \({}^{*}\)_ & Pyruvate dehydrogenase E2 & 1.32 \(\pm\) 0.25 & 0.93 \(\pm\) 0.15 \\ _acnB \({}^{*}\)_ & Aconitate hydratase B & 4.03 \(\pm\) 0.59 & 1.50 \(\pm\) 0.12 \\ _fumA \({}^{*}\)_ & Fumarase A & 0.77 \(\pm\) 0.03 & 1.44 \(\pm\) 0.03 \\ \hline \hline \end{tabular}
\end{table}