## 3 Results  
### The Antifungal Activity Assessment of LIN  
Our data indicated the promising antifungal effectiveness of LIN toward 16 reference fungal strains from four species: _Candida_ spp., _Cryptococcus_ spp., _Geotrichum_ spp., and _Saccharomyces_ spp. Considering the results shown in Table 1, this effect was demonstrated at MIC = 0.5-8 mg/mL. The MFCs were the same or 2-4-fold higher, in the range of 0.5-8 mg/mL. The strains belonging to _Cryptococcus_ spp. were found to be the most susceptible to LIN (MIC = MFC = 0.5 mg/mL, MFC/MIC = 1). _Geotrichum candidum_ strain showed a similar sensitivity at MIC = 0.5-1 mg/mL, and MFC = 1-2 mg/mL (MFC/MIC = 1-2). In turn, the activity of LIN toward _Candida_ spp. and _Saccharomyces_ spp. was slightly lower with MICs ranging from 1 to 8 mg/mL and MFCs from 2 to 8 mg/mL (MFC/MIC = 1-4). The antifungal activity of LIN against individual strains was similar. It should be added that studied monoterene showed a beneficial, fungicidal effect with MFC/MIC = 1-4 toward all reference strains. Our data showed the similar anticandid efficacy of LIN toward both tested groups of clinical yeasts: _C. albicans_ and other than _C. albicans_--NAC from hemato-oncology patients. The results, summarized in Table 2 and Figure 1, presented an antifungal activity of LIN with MIC in the range 0.5-8 mg/mL against all _Candidida_ spp. isolates. The MFCs were the same or 2-4 times higher than MICs, and their ranges were 1-16 mg/mL. Moreover, the minimum concentrations inhibiting the growth of 50% (MIC\({}_{50}\)) or 90% (MIC\({}_{90}\)) of all strains from both of the studied groups were 2 mg/mL and 8 mg/mL, respectively. \(\text{MFC}_{50}\) and \(\text{MFC}_{90}\) values were similar (\(\text{MFC}_{50}\) = 4 mg/mL and \(\text{MFC}_{90}\) = 8 mg/mL). \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multirow{2}{*}{**Reference Strains**} & \multicolumn{3}{c}{**LIN (mg/mL)**} & \multicolumn{3}{c}{**NYS (\#g/mL)**} \\ \cline{2-7}  & **Range** & **Range** & **MFC/MIC** & **MIC** & **MFC** & **MFC/MIC** \\  & **of MIC** & **of MFC** & **Ratio** & **MIC** & **MFC** & **Ratio** \\ \hline _Candidida albicans_ ATCC 10231 & 2–8 & 4–8 & 1–2 & 0.48 & 0.48 & 1 \\ _Candidida albicans_ ATCC 2091 & 2–4 & 4–8 & 2 & 0.24 & 0.24 & 1 \\ _Candidida albicans_ ATCC 64124 & 2 & 4 & 2 & 0.98 & 1.95 & 2 \\ _Candidida glabrata_ ATCC 90030 & 2–8 & 4–8 & 1–2 & 0.24 & 0.48 & 2 \\ _Candidida glabrata_ ATCC 15126 & 2 & 2 & 1 & 0.24 & 0.24 & 1 \\ _Candidida parapysilosis_ ATCC 22019 & 1–2 & 2–4 & 2 & 0.24 & 0.48 & 2 \\ _Candidida krusei_ ATCC 14243 & 1–4 & 4–8 & 2–4 & 0.24 & 0.24 & 1 \\ _Candidida kefyr_ ATCC 66028 & 4 & 4 & 1 & 0.24 & 0.48 & 2 \\ _Candidida lustimaine_ ATCC 34449 & 2 & 4 & 2 & 0.48 & 0.98 & 2 \\ _Candidida tropically_ ATCC 1369 & 1–2 & 2 & 1–2 & 0.24 & 0.48 & 2 \\ _Candidida auris_ CDC B11903 & 2–4 & 4 & 1–2 & 0.98 & 0.98 & 2 \\ _Cryptococcus neoformans_ ATCC 90112 & 0.5 & 0.5 & 1 & 0.12 & 0.24 & 2 \\ _Cryptococcus neoformans_ ATCC 32045 & 0.5 & 0.5 & 1 & 0.24 & 0.24 & 1 \\ _Cryptococcus gatti_ ATCC 56992 & 0.5 & 0.5 & 1 & 0.24 & 0.24 & 1 \\ _Geotrichum candidium_ ATCC 34614 & 0.5–1 & 1–2 & 1–2 & 0.98 & 0.98 & 1 \\ _Saccharomyces cerevisiae_ ATCC 9763 & 2 & 4 & 2 & 0.24 & 0.24 & 1 \\ \hline \hline \end{tabular}
\end{table} 
Table 1: The antifungal activity of LIN and NYS (nystatin as control) expressed as a range of MIC or MFC and \(\text{MFC}/\text{MIC}\) ratio toward the reference strains of yeasts. Figure 1: Distribution of MIC and MFC values of LIN among clinical isolates of _Candidida_ spp. The results also showed that the largest number of studied _Candida_ isolates was inhibited by LIN at MIC = 2-4 mg/mL (5 (25%)-7 (35%) strains). Based on MFCs, most of the isolates (7 each (35%) in both tested groups of yeasts) were killed at a minimal concentration of 8 mg/mL. Only single strains (1 (5%)-2 (10%) isolates) were especially sensitive to LIN at MIC = 0.5 mg/mL (Figure 1). Additionally, the evaluation of both MIC and MFC values of terpene allowed for the calculation of its MFC/MIC ratio (Table 2).