## 2 Materials and Methods
### Data Collection  
The study concerned the analysis of the data collected from patients with IBD. We obtained data about patients of the Department of Gastroenterology and Internal Diseases of the Medical University of Bialystok Clinical Hospital. The study involved interviews of adults and basic analysis of their medical records. The patients were diagnosed based on clinical symptoms, biochemical, radiological results, endoscopic findings, and histological reports. All patients were given CT examinations, for initial qualification. Information on the following laboratory results was collected: WBC (white blood cells) [\(x10^{3}/\mu\)L], RBC (red blood cells) [\(x10^{6}/\mu\)L], MCV (mean corpuscular volume) [\(f\)L], PLT [\(x10^{3}/\mu\)L], neutrophils [\(x10^{3}/\mu\)L], lymphocytes [\(x10^{3}/\mu\)L], monocytes [\(x10^{3}/\mu\)L], eosinophils [\(x10^{3}/\mu\)L], basophils [\(x10^{3}/\mu\)L], glucose [mg/dL], bilirubin [mg/dl], AspAT (aspartate aminotransferase) [\(|Ul/\)L], ALAT (alanine aminotransferase) [\(|Ul/\)L], amylase [\(|Ul/\)L], PT [sec], INR (international normalized ratio), fibrinogen [mg/dL], urea [mg/dL], creatinine [mg/dL], sodium [mmol/L], potassium [mmol/L], and CRP (C reactive protein) [mg/dL]. Additional features like age, gender, smoking (a smoker is a patient who smoked at least a year without interruption), occurrence of blood in the stool, and a palpable tumor within the abdominal cavity were taken into account. In the past, for CD and UC diagnosis, only laboratory tests were considered. In times of biological drugs, laboratory tests have become necessary to assess the burden of IBD, as symptom-based results are too subjective to predict properly the response to pharmacological options and calculate the risk of relapse. In this work, we were looking for features to distinguish easily CD from UC. Laboratory tests are helpful in assessing the activity of each disease. Morphological tests of blood and biochemical parameters determination allow early detection of changes, side effects of therapy, and monitoring of nutritional deficiencies. Below is a brief description of some data taken into account in our study [12]. Leukocytes (WBC) affect the immune system. Both excess and drop of white blood cells may indicate overload of the immune system, due to infections, diseases, including UC and CD. Erythrocytes (RBC) transport oxygen and carbon dioxide in the body, participating in gas exchange. A too small number of erythrocytes may indicate malnutrition, while higher values occur when there is a disturbance in the production of red blood cells in the course of IBD. Iron deficiency anemia is common in IBD. Elevated MCV occurs in patients receiving azathioprine or 6-mercaptoethanol. However, the roles, functions, and levels of monocytes in case of the UC and CD have not been fully examined [13,14]. Neutrophils are one of the groups of white blood cells (leukocytes). They play a significant role in the immune response. They are perceived as effector cells in acute and chronic inflammation. However, the roles of neutrophils in the pathogenesis and development of IBD and their differences between disease variants are still not fully understood [15]. Lymphocytes are cells of the immune system that belong to the leukocyte ag granulocytes to involve and underlie the immune response. CD and UC disease are characterized by dispersed accumulation of lymphocytes in the intestinal mucosa due to overexpression of endothelial adhesion molecules. It is important to know if there are any differences in the level of this parameter between UC and CD [13; 14]. Eosinophils play a role in the pathogenesis of IBD. Immunohistochemistryistopathological examinations revealed the accumulation and activation of eosinophils in the active inflammatory intestinal mucosa in patients with UC and CD. However, there is a lack of accurate quantitative data and their possible distinction in the two diseases analyzed in this paper [12]. Basophils belong to leukocytes.