## 3 Results
### Metabolite Identification Based on UPLC-Orbitrap-MS
** & **Rt** & **Molecular** & **Formula** & **Mode** & **Theoretical** & **Experimental** & **A (ppm) * & **Name** & **Fragments (Relative Intensity, \%)** & **Class** \\ \hline
1 & 9.867 & C\({}_{\rm B}\)H\({}_{\rm H}\)O\({}_{\rm G}\) & [M+H]\({}^{*}\) & 287.0550 & 287.0551 & \(-\)1.7418 & Kaempfer & 153.0184 (10), 121.0285 (10) & Flavonoid \\ \hline
2 & 9.567 & C\({}_{\rm B}\)H\({}_{\rm H}\)O\({}_{\rm G}\) & [M-H]\({}^{-}\) & 285.0399 & 285.0406 & 2.4558 & Latteolin & 197.2360 (10), 15.01024 (10), & Flavonoid \\ \hline
3 & 9.33 & C\({}_{\rm B}\)H\({}_{\rm H}\)O\({}_{\rm G}\) & [M+Na]\({}^{*}\) & 341.0273 & 341.0269 & \(-\)1.0556 & Myricetin & 319.0468 (10), 273.0399 (100), & Flavonoid \\
4 & 9.328 & C\({}_{\rm B}\)H\({}_{\rm H}\)\({}_{\rm 2}\)O\({}_{\rm G}\) & [M+H]\({}^{-}\) & 331.0454 & 331.0461 & 2.1145 & Laricitrin & 178.9987 (25), 151.0308 (25) & Flavonoid \\ \hline
5 & 8.712 & C\({}_{\rm B}\)H\({}_{\rm H}\)O\({}_{\rm G}\) & [M+H]\({}^{*}\) & 273.1121 & 273.1125 & \(-\)0.6225 & (\(\succ\))Vestiltil & 173.9799 (100), 102.1478 (10) & Isoflavane \\ \hline
6 & 9.114 & C\({}_{\rm B}\)H\({}_{\rm H}\)O\({}_{\rm G}\) & [M-H]\({}^{*}\) & 431.0978 & 431.0984 & 1.3686 & afazin & 285.0405 (200), 284.0324 (50), & Flavonoid \\
25 & 9.177 & C\({}_{\rm B}\)H\({}_{\rm H}\)O\({}_{\rm O}\) & [M-H]\({}^{-}\) & 447.0927 & 447.0934 & 1.5657 & Quercetin-3-O-rhammoside & 301.0359 (100), 300.0283 (25), & Flavonoid \\
2 & 8 & 8.551 & C\({}_{\rm B}\)H\({}_{\rm H}\)O\({}_{\rm O}\) & [M+H]\({}^{-}\) & 447.0927 & 447.0934 & 1.6775 & Kaempfer-3-O-rhammoside & 285.0426 (40), 285.0310 (100), & Flavonoid \\
2 & 8 & 8.551 & C\({}_{\rm B}\)H\({}_{\rm O}\)\({}_{\rm O}\) & [M+H]\({}^{-}\) & 447.0927 & 447.0934 & 1.6775 & Kaempfer-3-O-rhammoside & 285.0426 (40), 285.0310 (100), & Flavonoid \\
2 & 9 & 8.013 & C\({}_{\rm B}\)H\({}_{\rm O}\)\({}_{\rm O}\) & [M+H]\({}^{-}\) & 463.0877 & 463.0885 & 1.6843 & Hyperoside & 301.0371 (100), 300.0281 (60), & Flavonoid \\
2 & 10 & 7.621 & C\({}_{\rm B}\)H\({}_{\rm O}\)\({}_{\rm O}\)\({}_{\rm O}\)\({}_{\rm 2}\) & [M+H]\({}^{-}\) & 505.1346 & 505.1355 & 1.7421 & Junipegegenin B & 389.1245 (10), 341.1205 (60), & Isoflavane \\
2 & 11 & 7.894 & C\({}_{\rm B}\)H\({}_{\rm O}\)\({}_{\rm O}\)\({}_{\rm 4}\) & [M-H]\({}^{-}\) & 564.1401 & 563.1410 & 1.6515 & 3-O-xyloside-7-O-rhammoside & 195.0646 (40), 255.0301 (50) & glycoside \\ \hline
12 & 8.418 & C\({}_{\rm B}\)H\({}_{\rm O}\)\({}_{\rm H}\)O\({}_{\rm 4}\) & [M+H]\({}^{*}\) & 579.1708 & 579.1710 & \(-\)0.6043 & Kaempfer-3-O-rhammoside & 431.1339 (50), 287.0557 (100), & Flavonoid \\
13 & 9.674 & C\({}_{\rm B}\)H\({}_{\rm O}\)\({}_{\rm O}\)\({}_{\rm 15}\) & [M+H]\({}^{*}\) & 595.1657 & 595.1663 & \(-\)0.0840 & Kaempfer-3-O-rhammoside & 433.1134 (15), 287.0558 (100) & Flavond \\ \hline
14 & 7.784 & C\({}_{\rm B}\)H\({}_{\rm O}\)\({}_{\rm O}\)\({}_{\rm 19}\) & [M+H]\({}^{*}\) & 727.2080 & 727.2083 & \(-\)0.4813 & Kaempfer-3-O-rhammoside & 595.1716 (15), 433.1137 (15), & Flavond \\  & & & & & & & & & & \\  & & & & & & & & & & & \\  & & & & & & & & & & & & \\  & & & & & & & & & & & \\  & & & & & & & & & & & & \\ \hline
15 & 8.253 & C\({}_{\rm B}\)H\({}_{\rm O}\)\({}_{\rm 19}\) & [M+H]\({}^{*}\) & 74\(m/z\) 433 and 287, arising from the subsequent loss of deoxyhexose [M+H-146]\({}^{+}\) and [M+H-146-146]\({}^{+}\)[35]. A base peak at \(m/z\) 287 corresponds to the kaempfer aglycone, which compound 12 was tentatively identified as kaempfertrim. Compound **13** showed fragment ions at \(m/z\) 433 [M+H-162]\({}^{+}\) and \(m/z\) 287 [M+H-162-146]\({}^{+}\), indicating the loss of deoxyhexose and hexose moiety, respectively. Similarly, a base peak at \(m/z\) 287 was observed for compound **13**, which was identified as kaempfer-3-O-glucose-7-O-rhammoside [36]. Compound **14** showed a distinctive fragment ion at \(m/z\) 595 [M+H-132]\({}^{+}\) from the loss of pentose. The subsequent loss of deoxyhexose -146 Da and hexose -162 Da indicated fragment ions at \(m/z\) 433 and 287, respectively.