## 3 Results
### DSC Melting Profile Analysis
For the rest of the samples, four peaks were also recorded at a heating rate of 2 \({}^{\circ}\)C/min, as for a scanning rate of 1 \({}^{\circ}\)C/min. The first peak was observed at temperature T\({}_{\rm m}\)1 around \(-\)41 \({}^{\circ}\)C, and there were no significant differences except for sample HP_HLD. For the T\({}_{\rm m}\)2 of the second peak determined at around \(-\)31 \({}^{\circ}\)C, statistically significant differences were observed between the samples (\(p\leq\) 0.05). The lowest T\({}_{\rm m}\)2 value was observed for the HP_HLD sample at a temperature of \(-\)35.95 \({}^{\circ}\)C, and the highest value was recorded for HP_HLA at a temperature of \(-\)32.50 \({}^{\circ}\)C. The third peak on the curves appeared as a shoulder for all samples, for which the peak temperature (T\({}_{\rm m}\)3) was found to range from \(-\)22 \({}^{\circ}\)C to \(-\)26 \({}^{\circ}\)C. Similar to the 1\({}^{\circ}\)C/min heating rate, the fourth peak was the main peak (considering peak height and enthalpy values), except for the HP_HLA sample, for which the second peak dominated, with significantly different highest values of h2 and \(\Delta\)Hm2 (\(p\leq\) 0.05). The fourth peak (T\({}_{\rm m}\)4) for all samples was detected between \(-\)14 \({}^{\circ}\)C and \(-\)17 \({}^{\circ}\)C, whereas for the HP_HLD, sample the lowest peak temperature was observed, i.e., \(-\)17.36 \({}^{\circ}\)C; however, for the HP_HLE sample, the highest h4 value was obtained (0.34 W/g), which was statistically different (\(p\leq\) 0.05) from the other oil samples. Based on the results obtained with scanning rates of 1 and 2 \({}^{\circ}\)C/min, we also carried out an experiment with a faster heating rate of 5 \({}^{\circ}\)C/min, which resulted in a relatively fast analysis and prominent repeatability amongst the hemp seed oil samples (Figure 1C). Just as for the previous scanning rates, four peaks are visible, and the second peak is the most prominent for all samples. A comparison of the curves obtained at all scanning rates shows that the curves of all hemp seed oils are the most similar at a heating rate of 5 \({}^{\circ}\)C/min. Table 4 shows the values of the DSC parameters (T\({}_{\rm m}\), h, and \(\Delta\)H\({}_{\rm m}\)) calculated from curves obtained at a scanning rate of 5 \({}^{\circ}\)C/min. The first peak temperature (T\({}_{\rm m}\)1) appeared when the melting curves reached a temperature around \(-\)41\({}^{\circ}\)C, which was not significantly different for all oil samples. The second and major peak provides significantly different values (\(p\leq\) 0.05) for peak temperature (T\({}_{\rm m}\)2) and enthalpy (\(\Delta\)H\({}_{\rm m}\)2) between stored seed oils (HP_HLC and HP_HLD) and fresh seed oils (HP_HLA, HP_HLB, and HP_HLE), whereas for the first group of oils, T\({}_{\rm m}\)2 values were lower than for the second group of oils, which is a similar observation to that at a scanning rate of 2 \({}^{\circ}\)C/min. The peak height regarding values (h2) and enthalpy \(\Delta\)H\({}_{\rm m}\)2 values were the highest for the HP_HLA sample, i.e., 0.42 W/g and 30.14 J/g, respectively, and the lowest for the HP_HLE variety (0.3 W/g and 19.48 J/g, respectively). The third peak was generated when the temperature (T\({}_{\rm m}\)3) reached a value around \(-\)24 \({}^{\circ}\)C for all samples. In the case of assessing the fourth peak, the peak temperature (T\({}_{\rm m}\)4) was found to be the highest for the HP_HLA sample (\(-\)12.03 \({}^{\circ}\)C), while for the rest of the samples, it was between \(-\)17 and \(-\)19 \({}^{\circ}\)C. The peak height (h4) and enthalpy (\(\Delta\)H\({}_{\rm m}\)4) of the fourth peak were also the lowest and significantly different for those of HP_HLA, confirming that the lowest total content of polyunsaturated fatty acids (\(\Sigma\)PUFA), i.e., 74.14%, compared to the rest of oils samples can play a pivotal role in the shape of the DSC profile, especially in the case of the second and fourth peaks.