## 3 Results
### PDSC of Cellular Oil Samples  
In this experiment, PDSC measurements were conducted at a temperature of 120 \({}^{\circ}\)C at isothermal conditions. The PDSC curves for cellular lipids from M1-b, M2-fb and O1-fb are presented in Figure 3. It can be noted that the oxidation reaction time values for the extracted oil samples are different. At 120 \({}^{\circ}\)C, cellular oil from fed-batch culture with molasses (M2-fb) had the shortest Ton and Tmax lasting 2.58 min and 10.04 min, followed by oil from batch culture with molasses (M1-b) with results of about 8.41 min and 16.32 min, respectively. The highest value of T\({}_{\rm on}\) and T\({}_{\rm max}\) parameters are observed in the oil sample from the culture fed with post-frying rapeseed oil (O1-fb), which are 14.03 and 26.23 min.  
\begin{table}
\begin{tabular}{c c c c c c c c c c c c} \hline \hline \multirow{2}{*}{**Biomass Samples**} & **C** & **N** & **K** & **Na** & **P** & **S** & **Ca** & **Mg** & **Fe** & **Al** & **Ti** \\  & **g/kg** & **g/kg** & **g/kg** & **g/kg** & **g/kg** & **g/kg** & **g/kg** & **g/kg** & **g/kg** & **mg/kg** & **mg/kg** \\ \hline M1-b & 447.87 & 65.56 & 25.63 & 18.47 & 9.84 & 13.61 & 0.87 & 0.69 & 0.46 & 19.25 & 3.41 \\ M2-fb & 376.26 & 43.21 & 47.54 & 16.89 & 11.96 & 8.76 & 2.13 & 0.65 & 0.20 & 7.98 & 1.09 \\ O1-fb & 601.12 & 20.78 & 16.48 & 0.46 & 10.05 & 2.04 & 0.08 & 0.84 & 0.59 & 55.47 & 2.03 \\ \hline  & **Mn** & **Cu** & **Zn** & **Cr** & **Ni** & **Pb** & **V** & **Zr** & **Co** & **Sr** & **Ba** & **Li** \\  & mg/kg & mg/kg & mg/kg & mg/kg & mg/kg & mg/kg & mg/kg & mg/kg & mg/kg & mg/kg & mg/kg \\ \hline M1-b & 136.05 & 6.90 & 168.64 & 10.71 & 15.08 & 1.13 & 0.62 & 3.94 & 2.43 & 2.77 & 0.54 & 0.18 \\ M2-fb & 197.35 & 5.77 & 209.80 & 3.12 & 2.54 & 0.90 & 0.53 & 1.27 & 2.23 & 6.45 & 0.77 & 0.29 \\ O1-fb & 50.79 & 0.79 & 93.34 & 2.29 & 1.08 & 1.20 & 0.14 & 1.30 & 0.01 & 0.40 & 0.66 & 0.01 \\ \hline \hline \end{tabular}
\end{table}
Table 4: Elemental composition of dry biomass of _Y. lipolytica_ KKP 379 from M1-b, M2-fb and O1-fb media.  
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Oil Samples**} & \multicolumn{2}{c}{**M1-b**} & \multicolumn{2}{c}{**M2-fb**} & \multicolumn{2}{c}{**O1-fb**} \\ \cline{2-7}  & \% & **mg/g\({}_{\rm oil}\)** & **\%** & **mg/g\({}_{\rm oil}\)** & **\%** & **mg/g\({}_{\rm oil}\)** \\ \hline Cholesterol & 5.34 \(\pm\) 0.50 & 0.45 \(\pm\) 0.13 b & 0.32 \(\pm\) 0.05 & 0.22 \(\pm\) 0.04 a & 1.02 \(\pm\) 0.26 & 0.04 \(\pm\) 0.02 a \\ Dehydroregorosterol & 6.51 \(\pm\) 0.31 & 0.54 \(\pm\) 0.08 a & 1.85 \(\pm\) 0.24 & 1.26 \(\pm\) 0.11 b & 12.67 \(\pm\) 1.28 & 0.42 \(\pm\) 0.23 a \\ Ergosterol & 57.95 \(\pm\) 2.58 & 5.00 \(\pm\) 1.33 a & 87.94 \(\pm\) 0.91 & 60.16 \(\pm\) 3.33 b & 13.86 \(\pm\) 1.74 & 0.40 \(\pm\) 0.00 c \\ Campesterol & 10.24 \(\pm\) 0.91 & 0.90 \(\pm\) 0.10 a & 2.15 \(\pm\) 0.17 & 1.47 \(\pm\) 0.05 b & 25.22 \(\pm\) 3.22 & 0.84 \(\pm\) 0.49 a \\ Stigmasterol & 2.67 \(\pm\) 0.53 & 0.22 \(\pm\) 0.01 a & 2.90 \(\pm\) 0.38 & 1.98 \(\pm\) 0.17 b & 2.96 \(\pm\) 0.57 & 0.12 \(\pm\) 0.09 a \\ \(\beta\)-sitosterol & 18.25 \(\pm\) 3.81 & 1.50 \(\pm\) 0.02 a & 4.86 \(\pm\) 0.16 & 3.32 \(\pm\) 0.04 b & 44.29 \(\pm\) 0.38 & 1.44 \(\pm\) 0.69 a \\ \(\sum\) & 100 & 8.34 \(\pm\) 1.66 & 100 & 68.40 \(\pm\) 3.75 & 100 & 3.25 \(\pm\) 1.55 \\ \hline \hline \end{tabular}
\end{table}
Table 3: Content of the major sterols in microbial oil samples extracted from cultures of _Y. lipolytica_ KKP 379 biomass in medium M1-b, M2-fb and O1-fb.