## 3 Results
### Heparan Sulfate  
The dynamics of changes in the content of HS during the healing of experimental burn wounds on subsequent days of treatment with nonwoven dressings containing 5% and 10% propolis are illustrated in Figure 1. \begin{table}
\begin{tabular}{c c c c c} \hline \hline  & \multicolumn{3}{c}{**Content of HS [mg/g of Dry Tissue] in the Burn Wound**} \\ \hline
**Days of Experiment** & **NaCl (_n = 3_)** & **Nonwoven without** & **Nonwoven with** & **Nonwoven with** \\  & & **Proplis (_n = 3_)** & **5\% Propolis (_n = 3_)** & **10\% Propolis (_n = 3_)** \\ \hline Day 0 (normal skin) & 0.095 \(\pm\) 0.01 & 0.092 \(\pm\) 0.015 & 0.118 \(\pm\) 0.018 & 0.11 \(\pm\) 0.02 \\ Day 3 & 0.118 \(\pm\) 0.022 & 0.115 \(\pm\) 0.015 & 0.26 \(\pm\) 0.015 & 0.305 \(\pm\) 0.008 \\ Day 5 & 0.125 \(\pm\) 0.029 & 0.116 \(\pm\) 0.013 & 0.216 \(\pm\) 0.009 & 0.254 \(\pm\) 0.013 \\ Day 10 & 0.122 \(\pm\) 0.024 & 0.126 \(\pm\) 0.012 & 0.135 \(\pm\) 0.015 & 0.154 \(\pm\) 0.021 \\ Day 15 & 0.107 \(\pm\) 0.022 & 0.11 \(\pm\) 0.014 & 0.11 \(\pm\) 0.01 & 0.14 \(\pm\) 0.014 \\ Day 21 & 0.092 \(\pm\) 0.014 & 0.1 \(\pm\) 0.016 & 0.106 \(\pm\) 0.01 & 0.120 \(\pm\) 0.021 \\ \hline \hline \end{tabular}  
* The presented numerical values represent the arithmetic mean of three measurements made each day \(\pm\) standard deviation, taking into account the method of wound dressing.  
\end{table} 
Table 1: Content of HS in homogenates of tissue samples obtained from healthy skin and from burned wound beds on subsequent days of the wound healing process. \begin{table}
\begin{tabular}{c c c c c} \hline \hline  & \multicolumn{3}{c}{**Content of HA [mg/g of Dry Tissue] in the Burn Wound**} \\ \hline
**Days of Experiment** & **NaCl (_n = 3_)** & **Nonwoven without** & **Nonwoven with** & **Nonwoven with** \\  & & **Proplis (_n = 3_)** & **5\% Propolis (_n = 3_)** & **10\% Propolis (_n = 3_)** \\ \hline Day 0 (normal skin) & 0.646 \(\pm\) 0.036 & 0.575 \(\pm\) 0.02 & 0.677 \(\pm\) 0.029 & 0.680 \(\pm\) 0.04 \\ Day 3 & 0.716 \(\pm\) 0.072 & 0.83 \(\pm\) 0.055 & 1.464 \(\pm\) 0.076 & 1.865 \(\pm\) 0.087 \\ Day 5 & 0.773 \(\pm\) 0.04 & 1.187 \(\pm\) 0.033 & 2.767 \(\pm\) 0.09 & 3.261 \(\pm\) 0.11 \\ Day 10 & 0.903 \(\pm\) 0.038 & 1.869 \(\pm\) 0.125 & 3.371 \(\pm\) 0.076 & 3.996 \(\pm\) 0.222 \\ Day 15 & 1.234 \(\pm\) 0.041 & 1.842 \(\pm\) 0.064 & 2.874 \(\pm\) 0.094 & 3.199 \(\pm\) 0.086 \\ Day 21 & 1.275 \(\pm\) 0.038 & 1.675 \(\pm\) 0.042 & 2.746 \(\pm\) 0.049 & 3.093 \(\pm\) 0.104 \\ \hline \hline \end{tabular}  
* The presented numerical values represent the arithmetic mean of three measurements made each day \(\pm\) standard deviation, taking into account the method of wound dressing.  
\end{table} 
Table 2: The content of HA in homogenates of tissue samples obtained from healthy skin and from burned wound beds on subsequent days of the wound healing process. Statistical analysis revealed significant differences in the content of HS during the healing progression of burn wounds treated with a nonwoven dressing infused with both 5% and 10% propolisis. Specifically, on days 3 and 5 of the wound healing process, there was a substantial increase in heparan sulfate levels (\(p<0.001\)) within the burnt tissue compared to those present in healthy skin. Based on the obtained results, it was found that the content of HS in the wound bed differed significantly during the healing of the wound treated with a nonwoven dressing containing both 5% and 10% propolisis between the 3 rd and 5 th and the 5 th and 10 th day of therapy (\(p<0.001\)). The research showed that on day 15 (\(p<0.05\)) of the repair process, the content of HS in the wound treated with a biodegradable dressing enriched with 10% propolisis was significantly higher than in normal skin. The statistical evaluation revealed a significant increase in the content of HS in the wound bed compared to the control skin (\(p<0.05\)) on the 10 th day of post-burn wound treatment with a biodegradable dressing without propolisis.