## 1 Introduction  
Graviola, commonly called soursop belongs to the _Antonaceae_ family, whose scientific name is _Anmona muricata_. Graviola plays a crucial role in various traditional and alternative medicines. All parts of this evergreen tree which inhabits tropical and sub-tropical areas are used in natural medicine. The bark, leaves, roots, fruit and fruit seeds have their own respective use and specific properties and uses are attributed to different parts of the tree. The Graviola fruit is heart shaped and edible and together with its leaves, root and seeds contributes significantly in alternative medicine [1]. Generally, the fruit and fruit juice are used against worms and parasites in the body, against fever, to enhance mother's milk after childbirth and as an astringent for diarrhea and dysentery. The seeds are crushed and used against internal and external parasites, head lice and worms. The bark, leaves and roots are considered antispasmodic, hypotensive and sedative. The soursop tea prepared from the leaves and stem is consumed to provide relief for various disorders towards those effects. The fruits of Graviola are extensively used to make candies, syrups, ice creams, shakes and beverages. The fruit is also consumed as such. In the Brazilian Amazon soursop leaf tea is used to solve liver related problems. The oil extracted from the leaves and unripe fruit is mixed with olive oil and is used externally for neuralgia, rheumatism and arthritis pain.  
Ethano-medicinal activities in Africa and South America employ this plant source in their conventional medicine. Researchers have established the anticonvulsant, antiparasitic, anti-arthritic, antimalarial, antidiabetic hepato protective and anticancer properties of this plant. Biological and biochemical characterization indicated that annonceous acetogenins are the main ingredients of Graviola [2; 3; 4]. Predominantly, _Amona muricata_ or Graviola plays a key role in promoting anticancer activity. _A. muricata_ leaves have been subjected to investigation for a large number of human diseases, including cancer [5]. In the United States and Europe, Graviola is sold as a popular adjunctive natural therapy for cancer.  
The key biomolecules orchestrating the bioactive properties of Graviola are the acetogenins. These acetogenins have demonstrated selective toxicity to tumor cells at even trace dosages (1 part per million). Further the chemicals and acetogenins in Graviola have been demonstrated to also show strong anticancerous, antimuorous and antiviral properties too [6; 7; 8; 9; 10; 11; 12; 13; 14; 15; 16; 17]. With the huge back up and evidence regarding the medicinal aspects of Graviola, the soursop tea extracts prepared from the leaves has become a vouched-for commodity. Commercial sellers have commercialized products involving soursop leaf powders and tea bags for tea extracts. There is also the other product of selling dried leaves in traditional medicine shops. These leaves are also meant for soursop tea preparations.  
In the present work, we have attempted validation of one such dried soursop leaf product. We have prepared soursop tea using the conventional boil and add leaf procedure. We have also tried to push the extraction efficiency to the maximum using various others methods such as prolonged boiling, sonication, microwave extraction and a neoteric sono-microwave extraction methodology. We have attempted to reduce the extraction time using this introduced integrated technology. The enhanced bioactive properties as shown by total phenolics, flavanoids and antioxidant ability were validated for the five extraction methodologies compared. This is the first attempt in testing the efficacy and claim of a commercial soursop product.