## 3 Results and Discussion
### Enzymatic Hydrolysis Effect on Dietary Fiber Composition of SBP and SBP-CO\({}_{2}\)  
Enzymatic hydrolysis of berry and fruit pomace is often used to increase its SDF and oligosaccharidesacharide content. Therefore, the present study investigated the effect of enzymatic treatment on the changes in the fiber content of SBP and SBP-CO\({}_{2}\). Since pectin, hemicellulose and cellulose predominate in the polysaccharides of berry fiber, the main enzymatic activities of commercial enzymes selected for hydrolysis were cellulase (Celluclast(r) 1.5 L), endo-\(\beta\)-glucanase (Viscozyme(r) L), pectin lyase and pectinase (Pectinex(r) Ultra Tropical). The dietary fiber content and composition changes after hydrolysis are shown in Table 2.  
The hydrolysis with all commercial enzymes resulted in a decrease in TDF content. The enzymatic treatment with Viscozyme(r) L had the lowest effect on the IDF content of \begin{table}
\begin{tabular}{c c c} \hline
**Parameter** & **SBP** & **SBP-CO\({}_{2}\)** \\ \hline Moisture, \% & 4.17 \(\pm\) 0.04 a & 5.25 \(\pm\) 0.05 b \\ Crude protein & 21.09 \(\pm\) 0.28 a & 25.45 \(\pm\) 0.41 b \\ Lipids & 12.57 \(\pm\) 0.39 a & 1.80 \(\pm\) 0.20 b \\ Ash & 1.38 \(\pm\) 0.01 a & 1.53 \(\pm\) 0.03 b \\ TDF & 63.61 \(\pm\) 0.79 a & 70.65 \(\pm\) 0.56 b \\ IDF & 58.69 \(\pm\) 0.69 a & 63.93 \(\pm\) 0.68 b \\ SDF & 4.92 \(\pm\) 0.1 a & 6.72 \(\pm\) 0.44 b \\ SDF/IDF ratio & 1:12 & 1:9.5 \\
1 Mono- and oligosaccharidesacharides & 1.35 \(\pm\) 1.22 a & 0.57 \(\pm\) 0.45 a \\ \hline \end{tabular}
\end{table} 
Table 1: Proximate composition of SBP and SBP-CO\({}_{2}\), g/100 g d.m. \begin{table}
\begin{tabular}{c c c c c c c c} \hline  & \multicolumn{3}{c}{**SBP**} & \multicolumn{3}{c}{**SBP-CO\({}_{2}\)**} \\ \cline{2-9}  & **Control** & **Viscozyme® L** & **Pectinex®** & **Celluclast®** & **Control** & **Viscozyme®** & **Pectinex®** & **Celluclast®** \\  & **Control** & **Viscozyme®** & **LUTE** & **1.5 L** & **LUTE** & **LUTE** & **1.5 L** \\ \hline TDF, g/100 g d.m. & 63.61 \(\pm\) 0.79* & 58.85 \(\pm\) 0.31 b & 54.86 \(\pm\) 0.31 b & 52.9 \(\pm\) 0.31 a & 70.65 \(\pm\) 0.56 a & 63.09 \(\pm\) 0.28* & 61.37 \(\pm\) 0.21 a & 59.19 \(\pm\) 0.67 c \\ SDF, g/100 g d.m. & 4.92 \(\pm\) 0.1 c & 2.90 \(\pm\) 0.02 a & 3.52 \(\pm\) 0.31 a & 4.68 \(\pm\) 0.62 b & 6.72 \(\pm\) 0.44 d & 4.22 \(\pm\) 0.48 c & 4.96 \(\pm\) 0.12 c & 6.41 \(\pm\) 0.53 d \\ IDF, g/100 g d.m. & 58.69 \(\pm\) 0.69 a & 52.95 \(\pm\) 0.61 c & 51.34 \(\pm\) 0.59 b & 482.2 \(\pm\) 0.31 a & 63.93 \(\pm\) 0.68 b & 58.87 \(\pm\) 0.08 a & 56.41 \(\pm\) 0.31 a & 52.78 \(\pm\) 0.02 c \\ SDF/IDF ratio & 1:12 & 1:18:2 & 1:14:6 & 1:10:3 & 1.95 & 1:13:9 & 1:11.4 & 1.82 \\ TPC, mg CAE/g & 5.73 \(\pm\) 0.02 a & 5.96 \(\pm\) 0.02 d & 6.05 \(\pm\) 0.01 s & 6.15 \(\pm\) 0.02 b & 4.64 \(\pm\) 0.01 a & 4.81 \(\pm\) 0.01 b & 4.85 \(\pm\) 0.01 c & 4.92 \(\pm\) 0.01 d \\ \hline \end{tabular}
\end{table} 
Table 2: Enzymatic hydrolysis effect on the amount and composition of dietary fibers and total phenolic content (TPC) in SBP and SBP-CO\({}_{2}\). SBP and SBP-CO\({}_{2}\) (IDF decreased by 12.2% and 10.7%, respectively). While treatment with Celluclast\({}^{\circledR}\) 1.5 L hydrolyzed the most IDF (16.8% and 16.2% for SBP and SBP-CO\({}_{2}\), respectively). In SBP and SBP-CO\({}_{2}\) samples, the most pronounced degradation of SDF was achieved with Viscozyme\({}^{\circledR}\) L, resulting in reductions of 41.05% and 37.2%, respectively. In contrast, hydrolysis with Celluclast\({}^{\circledR}\) 1.5 L had the least effect, with corresponding reductions of only 4.9% and 4.6%. The results demonstrated that enzyme preparation mainly composed of cellulose-degrading enzyme--cellulase (Celluclast\({}^{\circledR}\) 1.5 L) was the most effective in hydrolyzing IDF, while enzyme preparations containing pectinases (Viscozyme\({}^{\circledR}\) L and Pectinex\({}^{\circledR}\) Ultra Tropical) were the most effective in degrading SDF.