## 3 Results and Discussion
### In Vitro Antifungal Assays  
From the isolates obtained, Aspergillus candidus, Aspergillus pseudogalucus, Penicillin brevicompactum, Talaromyces coalescens and Trichoderma longbrachiatum were selected to be exposed to EO vapors from Cymbopogon citratus, Lavandula angustifolia, Thapsia gummifera, Mentha pulegium, Mentha suaveolens, Thapsia villosa, Thymus mastichina and Agastache foeniculum at four different concentrations: 0.5, 1, 1.5 and 2 \(\mu\)L cm\({}^{-3}\). The diameter of the colonies was measured every 7 days, for a total of 6 weeks (Figures 3-7). All EOs, at each tested concentration, had fully evaporated by the time the first measurements took place. Figure 3: _Aspergillus candidus_ average growth curves (n = 3) over 42 days after exposure to the volatilized fraction of the different essential oils. Concentration is given in \(\mu\)L cm\({}^{-3}\). The control group (Concentration 0.0) was shared across all treatments. The shaded area represents the Min–Max range of results per tested concentration. For each EO, data (n = 3) associated with different letters represent significantly different results at the end of the experiment, in accordance with [PERSON]’s post hoc test, \(p\) < 0.05. In the case of _A. candidus_ (Figure 2), the growth curves for the colonies exposed to _T. villosa_, _T. gummifera_, _M. pulegium_, _T. mastichina_ and _A. foncticulum_ EOs show a similar trend to that of the control group, even if some significant differences were observed between 2 \(\mu\)L cm\({}^{-3}\) and the control for some of these EOs. _Mentha pulegium_ at 1, 1.5 and 2 \(\mu\)L cm\({}^{-3}\) seems to delay fungal growth to a certain extent, with statistical analysis showing that the highest concentration had a significant impact on colony diameter. The other _Mentha_ EO, namely _M. suaveolens_, also displays this same effect for the two lowest concentrations  
Figure 4: _Aspergillus pseudoglaucus_ average growth curves (n = 3) over 42 days after exposure to the volatilized fraction of the different essential oils. Concentration is given in \(\mu\)L cm\({}^{-3}\). The control group (Concentration 0.0) was shared across all treatments. The shaded area represents the Min–Max range of results per tested concentration. For each EO, data (n = 3) associated with different letters represent significantly different results at the end of the experiment, in accordance with [PERSON]’s post hoc test, \(p\) < 0.05.  
tested, with the highest ones completely inhibiting colony growth. _L. angustifolia_ EO also had a clear impact on fungal development, with no growth registered for concentrations above 0.5 \(\upmu\)L cm\({}^{-3}\). Nevertheless, only _C. citratus_ completely inhibited _A. candidus_ growth at all tested concentrations. _P. brevicompactum_ Colony Growth Over Time for Different Essential Oils  
Figure 5: _Penicillium brevicompactum_ average growth curves (n = 3) over 42 days after exposure to the volatilized fraction of the different essential oils. Concentration is given in \(\upmu\)L cm\({}^{-3}\). The control group (Concentration 0.0) was shared across all treatments. The shaded area represents the Min–Max range of results per tested concentration. For each EO, data (n = 3) associated with different letters, represent significantly different results at the end of the experiment, in accordance with [PERSON]’s post hoc test, \(p\) < 0.05. For _A. pseudoglaucus_ (Figure 3), two EOs (_C.citratus_ and _L. angustifolia_) were able to completely inhibit fungal growth even at the lowest tested concentration. Both _Mentha_ EOs tested showed the same ability to inhibit fungal growth, however with varying degrees of success, with _M. suaveolens_ only being able to do so at the two highest tested concentrations, while _M. pulgeium_ showed this effect for all concentrations above 0.5 \(\mu\)L cm\({}^{-3}\). Nevertheless, statistical analysis showed that the concentrations of 1, 1.5 and 2 \(\mu\)L cm\({}^{-3}\) were significantly similar to each other and different from the control group for both of these EOs.