## 2 Results
### Proteome Profiling of Macro-Mutant Lines
#### 2.2.2 Identification and Expression Patterns of Differentially Expressed Proteins of Mutant lines
\begin{table}
\begin{tabular}{c c c c c c c c c c c} \hline \hline
**Spot** & **Protein** & **MaxSOT** & **Normal** & **pl** & **Sequence** & **Protein Name** & **Protein Function** & **Sub-Cellular** & **Pathway/Biological** & **Accession** \\
**No.** & **View** & **Score** & **Mass (Da)** & **pl** & **Method (\%)** & **Protein Name** & **Protein Function** & **Sub-Cellular** & **Pathway/Biological** & **Accession** \\ \hline
7315 & EFIQLARATH & 125 & 36388 & 6.50 & 58 & \
\begin{tabular}{c} Eukaryotic translation \\ initiation factor 3 \\ subunit \\ 2(eFI31/TRIP-1) \\ \end{tabular} &
\begin{tabular}{c} Component of eukaryotic \\ initiation factor 3 (eFI-3) \\ \end{tabular} & Cytoplasm & Protein synthesis & Q38884 \\ \hline
2401 & AOC3\_ARATH & 66 & 28015 & 9.19 & 43 & Allee oxide cyclase 3 & \
\begin{tabular}{c} It is involved in the \\ production of \\ 2-oxo-pytholenoic acid, \\ a precursor of isomamic \\ acid. \\ \end{tabular} & Chloroplast &
\begin{tabular}{c} Jasmonic acid \\ biosynthesis process \\ \end{tabular} & QPLS01 \\ \hline
3001 & DUT\_ARATH & 70 & 17603 & 5.3 & 51 & \
\begin{tabular}{c} Ile produces dUMP, the \\ immediate precursor of \\ 5’-tiphosphate \\ undecidello \\ undecidello \\ undecidello \\ \end{tabular} & Cytoplasm &
\begin{tabular}{c} Nucleotide \\ metabolism \\ (pyrimidine \\ metabolism) \\ \end{tabular} & Q9 ST66 \\ \hline
3009 & RAN1\_ORYS1 & 120 & 25275 & 6.38 & 56 & \
\begin{tabular}{c} GTP-binding nuclear \\ protein Ran-1 \\ \end{tabular} &
\begin{tabular}{c} It is involved in \\ nucleocyte-plastic \\ transcript. \\ \end{tabular} & Nucleus & Protein import into \\ \hline
4006 & FIEL\_ORYSJ & 99 & 52,100 & 7.58 & 44 & \begin{tabular}{c} Polycomb group \\ protein FIE1 \\ \end{tabular} & \
\begin{tabular}{c} It is involved in cell \\ differentiation, \\ endometrium development, \\ and seed development. \\ \end{tabular} & Nucleus &
\begin{tabular}{c} Differentiation, \\ Transcription, \\ Transcription \\ regulation \\ \end{tabular} & Q6 ZIP8 \\ \hline
4518 & PREI\_ARATH & 120 & 10,560 & 9.00 & 79 & Transcription factor & \
\begin{tabular}{c} It integrates multiple \\ signaling pathways to \\ regulate cell elongation \\ and plant development. \\ \end{tabular} & Nucleus &
\begin{tabular}{c} Gibberell acid \\ mediated signaling \\ pathway \\ \end{tabular} & Q9 FLE9 \\ \hline
4900 & V5 REB1\_NICSA & 51 & 23010 & 5.67 & 72 & \begin{tabular}{c} Geranyl diphosphate \\ synthase \\ \end{tabular} & \
\begin{tabular}{c} It is involved in the \\ isoprendol biosynthetic \\ process \\ \end{tabular} & Cytoplasm &
\begin{tabular}{c} Key gene of \\ monotonepene \\ pathway \\ \end{tabular} & V5 REB1 \\ \hline \end{tabular}
\end{table} 
Table 4: _Cont._ \begin{table}
\begin{tabular}{l c c c c c c c c c} \hline \hline \multirow{2}{*}{**Protein Name**} & \multicolumn{6}{c}{**Expression Pattern of Proteins in Leaves of Control and Mutant Lines of Nigella sativa L.**} \\ \cline{2-9}  & **Control** & **HY1** & **HY3** & **MHY2** & **MHY4** & **NY3** & **LY1** & **LY3** & **VLY2** & **VLY3** \\ \hline DNA-(apurinic or & & & & & & & & & \\ apyrimidinic site) lyase & & & & & & & & & \\
1-aminocyclopropane-1- & & & & & & & & & \\ carboxylate oxidase 2 & & & & & & & & & \\ L-ascorbate peroxidase (APX1) & & & & & & & & \\ Manganese superoxide & & & & & & & & \\ dismutase (MSD1) & & & & & & & & \\ Glyceraldehyde 3- & & & & & & & & \\ phosphate & & & & & & & & \\ dehydrogenase A & & & & & & & & \\ \hline Tubulin alpha-6 chain & & & & & & & & \\ (TUA6) & & & & & & & & \\ Eukaryotic translation & & & & & & & & \\ initiation factor 3 subunit & & & & & & & & \\
2 (eIF3I)/TRIP-1) & & & & & & & & \\ Allene oxide cyclase 3 & & & & & & & & \\ Deoxyuridine & & & & & & & & \\
5′-triphosphate & & & & & & & & \\ nucleotid hydrolase & & & & & & & & \\ GTP-binding nuclear & & & & & & & & \\ protein Ran-1 & & & & & & & & \\ \hline \hline \end{tabular}
\end{table} 
Table 5: _Cont._