## 3 Results
### Multivariate Metabolomics Data Analysis  
Metabolite profiling of inflorescences from five different cultivars of _C. sativa_ revealed a large number of volatile and non-volatile compounds. These data were subjected to multivariate statistical analysis to understand the clustering and correlations between the investigated samples. In Figure 2A, an explorative overview of the chemical composition (mean percentage values) is reported to visualize the most representative volatile and non-volatile compounds in each cultivar. As far as is concerned the volatile components, the most relevant are \(\beta\)-myrcene, \(\beta\)-caryophyllene, humulene, selina-3,7(11)-diene and limonene, which are identified in all the cultivars. In Green Poison, 17 compounds were detected of which \(\beta\)-caryophyllene (34.5%), selina-3,7(11)-diene (17.1%) and humulene (11.4%) were the top three. \(\beta\)-Caryophyllene also reached the highest percentage value in Banana Hybrid (28.9%) followed by \(\beta\)-myrcene (25.0%) and \(\alpha\)-pinene (13.7%) for a total of 18 identified components. As noticeable in Figure 2A, \(\alpha\)-pinene was also detected in Gorilla CBD where a total amount of 19 compounds were identified including \(\beta\)-myrcene which reached 40.8% of the total amount. Similarly, \(\beta\)-myrcene with a percentage value of 29.6%, followed by \(\beta\)-caryophyllene (27.6%) and selina-3,7(11)-diene (12.2%) were found in V1 CBD. Candy BUD was characterized by 20 compounds and the most meaningful were \(\beta\)-caryophyllene (21.6%), selina-3,7(11)-diene (16.7%) and \(\beta\)-myrcene (15.9%). Regarding \begin{table}
\begin{tabular}{c c c c c c c} \hline
**No.** & **COMPONENT 1** & **Green Poison** & **V1 CBD** & **Banana Hybrid** & **Gorilla CBD** & **Candy BUD** \\ \hline
36 & 1,5-anhydro-2,4,6-tris-O-(3,4,5-trihydroxybenzoyl)-D-glucitol & 0.5 \(\pm\) 0.02 & - & - & - & 0.1 \(\pm\) 0.01 \\
37 & 3,19-diacetoxy-5,14-dihydroxycard-20(22)- & 1.1 \(\pm\) 0.05 & - & - & - & - \\  & 5,7,4’-trihydroxy-3,6-dimethoxy-3’,5’-dipreinylflavone & 1.6 \(\pm\) 0.077 & - & - & - & - \\
38 & 39 & acovenoside A & 2.3 \(\pm\) 0.10 & 2.0 \(\pm\) 0.02 & - & - & - \\
40 & cannabilactone & - & 3.8 \(\pm\) 0.05 & - & - & - \\
41 & cidyrosamidin & - & & - & 2.0 \(\pm\) 0.13 & - \\
42 & kushenol A & - & 2.9 \(\pm\) 0.04 & - & - & - \\
43 & neoadjuvantrographide & 2.4 \(\pm\) 0.11 & - & - & - & - \\  & (IS,2S,4R)-1,3,3- & & & & & \\  & trimethylbicyclo[2.2.1]hept-2-yl & & & & & \\
44 & (6a,10 ar)-1-hydroxy-6,6,9-trimethyl-3- & 0.6 \(\pm\) 0.03 & - & - & 2.1 \(\pm\) 0.14 & - \\  & pentyl-6a,7,8,10a-tetrahydro-6H- & & & & & \\ benzo[c]chrome-2-carboxylate & & & & & & \\
45 & ascipen & 2.2 \(\pm\) 0.10 & 1.1 \(\pm\) 0.01 & - & - & - \\
46 & baliosgermin & 1.6 \(\pm\) 0.07 & - & - & - & - \\
47 & conferoside & - & & - & 13.8 \(\pm\) 0.92 & - \\  & SUM & 99.1 & 92.9 & 98.7 & 99.1 & 99.1 \\ \hline \end{tabular} 1  
\end{table} 
Table 2: _Cont._non-volatile compounds, the largest number of molecules was detected in both Banana Hybrid (27) and Green Poison (30). Otherwise, 26 metabolites in V1 CBD, 24 in Candy BUD and 19 in Gorilla CBD were revealed. Of all of them, \(\delta\)-9-cis-tetrahydrocannabinol was the most significant with the only exception for V1 CBD in which the major component, ananolignan J, reached 14.1%. Figure 2: (**A**). Bar plot of the chemical composition (percentage mean value) of _C. sativa_ infloresances for volatile and non-volatile compounds. (**B**). Heatmap of normalized by sum metabolites percentage in five cultivars of _C. sativa_ L. Each cultivar is indicated in the column, every row indicates a compound. Red indicates high abundance, whereas compounds under the detection threshold are in gray. The complete list of volatile and non-volatile metabolites, normalized by sum, was also reported in a heatmap (Figure 2B). Heatmap analysis of the five different cultivars revealed that the metabolites \(\delta\)-9-cis-tetrahydrocannabinol, \(\beta\)-caryophyllene and \(\beta\)-myrcene showed the most significant percentage.