## 3 Results
### Revealing the Impact of Allergen Exposure on Epithelial Barrier Integrity Through TEER Monitoring
\begin{table}
\begin{tabular}{c c c c} \hline
**Exposure Condition** & \begin{tabular}{c} **Changes in** \\ **Normalized** \\ **TER (\%) at 72 h** \\ \end{tabular} & \
\begin{tabular}{c} **Tight Junction Integrity** \\ **(ZO-1 IF Staining \({}^{*}\))** \\ \end{tabular} & **Implications for Airway Health** \\ \hline AM 100 (100 \(\mu\)g/mL) & 74.15\(\%\)\(\pm\) 6.14\% & 1: Mild ZO-1 disruption & Mild barrier dysfunction, \\  & & increased permeability \\ \hline DAM 50 (50 \(\mu\)g/mL) & 76.54\(\%\)\(\pm\) 4.17\% & 1: Mild ZO-1 disruption & Mild barrier dysfunction, \\  & & increased permeability \\ \hline DAM 100 (100 \(\mu\)g/mL) & 64.95\(\%\)\(\pm\) 5.05\% & 2: Moderate ZO-1 disruption & Enhanced permeability, potential \\  & & for allergen penetration \\ \hline RW 200 (200 \(\mu\)g/mL) & 52.14\(\%\)\(\pm\) 4.88\% & 3: Severe ZO-1 disruption & Marked permeability, greater \\  & & potential for allergen penetration \\  & & and for exacerbated \\  & & allergic response \\ \hline HDM 200 (200 \(\mu\)g/mL) & 47.87\(\%\)\(\pm\) 3.91\% & 3: Severe ZO-1 disruption & Marked permeability, greater \\  & & potential for allergen penetration \\  & & and for exacerbated \\  & & allergic response \\ \hline HR 50 (RW + HDM 50 \(\mu\)g/mL) & 68.81\(\%\)\(\pm\) 3.27\% & 2: Moderate ZO-1 disruption & Enhanced permeability, potential \\  & & for allergen penetration \\ \hline HR 100 (RW + HDM 100 \(\mu\)g/mL) & 51.86\(\%\)\(\pm\) 4.98\% & 3: Severe ZO-1 disruption &
\begin{tabular}{c} Marked permeability, greater \\ potential for allergen penetration \\ and for exacerbated \\ allergic response \\ \end{tabular} \\ \hline HR 200 (RW + HDM 200 \(\mu\)g/mL) & 44.18\(\%\)\(\pm\) 6.19\% & 3: Severe ZO-1 disruption & Marked permeability, greater \\  & & potential for allergen penetration \\  & & and for exacerbated \\  & & allergic response \\ \hline \end{tabular}  
* ZO-1 disruption is defined as a loss of continuous membrane staining, redistribution of ZO-1 to the cytoplasm and a fragmented or punctate staining pattern (0: intact ZO-1: 1 partial ZO-1 disruption; 2: important ZO-1 disruption; 3: widespread ZO-1 disruption). The groups are labeled as follows: CTR: control; RW 200: RW extract 200 \(\mu\)g/mL; HDM 200: HDM extract 200 \(\mu\)g/mL; HR 200: RW + HDM extracts in equal parts at 200 \(\mu\)g/mL; HR 100: RW+HDM extracts 100 \(\mu\)g/mL; HR 50: RW+HDM 50 \(\mu\)g/mL; HR 25: RW+HDM 25 \(\mu\)g/mL; DAM 100: Der p 1+Amb a 11+Amb a 11+Amb a 12 preparation corresponding to 100 \(\mu\)g/mL of allergen extract; DAM 50: Der p 1+Amb a 1+Amb a 11+Amb a 12 preparation corresponding to 50 \(\mu\)g/mL of allergen extract; AM 100: Amb a 1+Amb a 11+Amb a 12 preparation corresponding to 100 \(\mu\)g/mL of allergen extract.  
\end{table} 
Table 1: _Cont._  
Figure 5: Visualization of the immunofluorescence intensity changes of ZO-1 tight junctions across all treatment groups. The fluorescence intensity of ZO-1 junctions was measured in ImageJ.JS 1.53 msoftware and are displayed as a percentage of the mean value of the control. The results are expressed as the mean \(\pm\) SD. \(p<0.0001\) versus control for all treatment groups. The groups are labeled as follows: CTR: control; RW 200: RW extract 200 \(\mathrm{\SIUnitSymbolMicro\text{$\mu$g}}\)/mL; HDM 200: HDM extract 200 \(\mathrm{\SIUnitSymbolMicro\text{$\mu$g}}\)/mL; HR 200: RW + HDM extracts in equal parts at 200 \(\mathrm{\SIUnitSymbolMicro\text{$\mu$g}}\)/mL; HR 100: RW+HDM extracts 100 \(\mathrm{\SIUnitSymbolMicro\text{$\mu$g}}\)/mL; HR 50: RW+HDM 50 \(\mathrm{\SIUnitSymbolMicro\text{$\mu$g}}\)/mL; HR 25: RW+HDM 25 \(\mathrm{\SIUnitSymbolMicro\text{$\mu$g}}\)/mL; DAM 100: Der p 1+Amb a 11+Amb a 12 preparation corresponding to 100 \(\mathrm{\SIUnitSymbolMicro\text{$\mu$g}}\)/mL of allergen extract; DAM 50: Der p 1+Amb a 1+Amb a 11+Amb a 11+Amb a 12 preparation corresponding to 50 \(\mathrm{\SIUnitSymbolMicro\text{$\mu$g}}\)/mL of allergen extract; AM 100: Amb a 1+Amb a 11+Amb a 11+Amb a 12 preparation corresponding to 100 \(\mathrm{\SIUnitSymbolMicro\text{Figure 5:0g}}\)/mL of allergen extract.