## 4 Discussion  
After the tests were performed on the delimited surfaces of the 80- to 100-year-old clothing object, the conclusion was drawn that all the EOs had a strong inhibitory effect on the fungi present, even if they were applied on different materials (cotton yard, leather, wool, yearly beads). This is due to the fungicidal effect of EOs, which persists in some cases over 30 days after application. Similar results were obtained by different authors [82; 83; 84; 85] following the application of these extracts on various materials. Most EOs (except for _Citrus limon_ and _Mentha piperita_) were observed to have relatively little effect on _Botrytis_ sp., which might indicate some resistance of this type of fungus to the action of the substances concerned. It was revealed that the genus _Cidosporium_ sp. developed at 5 days of incubation on the culture medium shown with the sample taken 48 h after the application of the EO, without the species involved having been isolated from the primary sample (taken before the application of the EO). The sample concerned was taken from the wool threads of the inner region of the coat. Woollen garments seem to be easily contaminated with fungal spores, with the degree of fungal contamination being most likely to differ from layer to \begin{table}
\begin{tabular}{c c c} \hline \hline
**Essential Oil Used** & **Inhibited Fungal Species** & **Duration of the Inhibitory Effect from the Moment of Application** \\ \hline _Lavandula angustiglia_ & _Botrytis_ sp. & Up to 22 days \\ \hline _Citrus limon_ & _Botrytis_ sp. & Minimum of 32 days \\ \hline _Mentha piperita_ & _Botrytis_ sp. & Minimum of 32 days \\ \hline \multirow{3}{*}{_Melaleuca alternifolia_} & _Candidia guilliermondii_ & Minimum of 32 days \\  & _Mucor_ sp. & Up to 22 days \\  & _Pentiillium_ sp. & Minimum of 30 days \\ \hline \multirow{3}{*}{_Marjoram_} & _Alternaria_ sp. & Minimum of 30 days \\  & _Apergillus_ sp. & Minimum of 30 days \\  & _Botrytis_ sp. & Up to 22 days \\ \hline \multirow{3}{*}{_Origanum vulgare_} & _Cidosporium_ sp. & Minimum of 30 days \\  & _Botrytis_ sp. & Up to 22 days \\ \cline{1-1}  & _Pentiillium_ sp. & Minimum of 30 days \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Fungal species inhibited by tested essential oils and the tested duration of their inhibitory effect. \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Samples Taken** & **Examined Area** & **Essential Oil** & **Fungal Colony Growth** \\ \hline \multirow{6}{*}{30 min after application of the essential oils} & Area A & _Lavandula angustiglia_ & Absent \\  & Area B & _Citrus limon_ & Absent \\  & Area C & _Mentha piperita_ & Absent \\  & Area D & _Melaleuca alternifolia_ & _Mucor_ sp. \\  & Area E & _Marjoram_ & Absent \\  & Area F & _Origanum vulgare_ & _Botrytis_ sp. \\ \hline \multirow{6}{*}{24 h after the application of the essential oils} & Area A & _Lavandula angustiglia_ & Absent \\  & Area B & _Citrus limon_ & Absent \\  & Area C & _Mentha piperita_ & Absent \\  & Area D & _Melaleuca alternifolia_ & _Mucor_ sp. \\  & Area E & _Marjoram_ & Absent \\  & Area F & _Origanum vulgare_ & _Botrytis_ sp. \\ \hline \multirow{6}{*}{48 h after the application of the essential oils} & Area A & _Lavandula angustiglia_ & _Botrytis_ sp. \\  & Area B & _Citrus limon_ & Absent \\ \cline{1-1}  & Area C & _Mentha piperita_ & Absent \\ \cline{1-1}  & Area D & _Melaleuca alternifolia_ & _Cladosporium_ sp. \\ \cline{1-1}  & Area E & _Maryam_ & _Botrytis_ sp. \\ \cline{1-1}  & Area F & _Origanum vulgare_ & _Botrytis_ sp. \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Interpretation of the seeded plates, with samples taken 30 min, 24 h and 48 h after applying the essential oil. layer--superficial, intermediate and basal. The spores that are present in the lower layers may rise to the surface over time, and, if the EO is applied superficially or in insufficient quantity, its inhibitory effect is diminished.