# SPME Method Optimized by Box-Behnken Design for Impact Odorants in Reduced Alcohol Wines
## 3 Results and Discussion
### Volatile Changes after RO-EP Treatment
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline  & \multicolumn{3}{c}{**Dealcoholized and Natural Wines**} & \multicolumn{2}{c}{**Reconstituted Wines**} \\ \cline{2-6}
**Compound (ug L \({}^{-1}\))** & \multicolumn{1}{c}{**5\% v/v**} & \multicolumn{1}{c}{**8\% v/v**} & \multicolumn{1}{c}{**13.7\% v/v**} & \multicolumn{1}{c}{**24 h**} & \multicolumn{1}{c}{**14 Days**} \\ \hline ethyl butyrate & 0.90 \(\pm\) 0.01 \({}^{\mathrm{a}}\) & 1.72 \(\pm\) 0.08 \({}^{\mathrm{b}}\) & 2.69 \(\pm\) 0.06 \({}^{\mathrm{c}}\) & 0.75 \(\pm\) 0.01 \({}^{\mathrm{a}}\) & 0.76 \(\pm\) 0.02 \({}^{\mathrm{a}}\) \\ ethyl1-2-methyl butyrate & 0.33 \(\pm\) 0.00 \({}^{\mathrm{b}}\) & 0.46 \(\pm\) 0.03 \({}^{\mathrm{c}}\) & 0.47 \(\pm\) 0.01 \({}^{\mathrm{c}}\) & 0.23 \(\pm\) 0.01 \({}^{\mathrm{a}}\) & 0.17 \(\pm\) 0.00 \({}^{\mathrm{a}}\) \\ ethyl3-methyl butyrate & 0.05 \(\pm\) 0.00 \({}^{\mathrm{b}}\) & 0.08 \(\pm\) 0.01 \({}^{\mathrm{c}}\) & 0.08 \(\pm\) 0.00 \({}^{\mathrm{c}}\) & 0.04 \(\pm\) 0.00 \({}^{\mathrm{b}}\) & 0.03 \(\pm\) 0.00 \({}^{\mathrm{a}}\) \\ isoamyl acetate & 0.21 \(\pm\) 0.01 \({}^{\mathrm{b}}\) & 0.43 \(\pm\) 0.03 \({}^{\mathrm{c}}\) & 0.51 \(\pm\) 0.03 \({}^{\mathrm{c}}\) & 0.08 \(\pm\) 0.01 \({}^{\mathrm{a}}\) & 0.08 \(\pm\) 0.01 \({}^{\mathrm{a}}\) \\ 3-methyl-1-butanol & 6.18 \(\pm\) 0.06 \({}^{\mathrm{a}}\) & 6.64 \(\pm\) 0.41 \({}^{\mathrm{b}}\) & 9.05 \(\pm\) 0.24 \({}^{\mathrm{c}}\) & 5.62 \(\pm\) 0.17 \({}^{\mathrm{ab}}\) & 5.38 \(\pm\) 0.13 \({}^{\mathrm{a}}\) \\ ethyl hexanoate & 1.72 \(\pm\) 0.02 \({}^{\mathrm{a}}\) & 2.34 \(\pm\) 0.23 \({}^{\mathrm{a}}\) & 5.41 \(\pm\) 0.08 \({}^{\mathrm{b}}\) & 1.38 \(\pm\) 0.11 \({}^{\mathrm{a}}\) & 1.51 \(\pm\) 0.05 \({}^{\mathrm{a}}\) \\ ethyl-1-lactate & 33,500 \(\pm\) 7.20 \({}^{\mathrm{a}}\) & 32,300 \(\pm\) 2.800 \({}^{\mathrm{a}}\) & 47,300 \(\pm\) 300 \({}^{\mathrm{b}}\) & 41,900 \(\pm\) 1870 \({}^{\mathrm{ab}}\) & 33,900 \(\pm\) 1560 \({}^{\mathrm{a}}\) \\ (z)-3-hexenol & 5.32 \(\pm\) 0.10 \({}^{\mathrm{c}}\) & 5.42 \(\pm\) 0.04 \({}^{\mathrm{c}}\) & 6.83 \(\pm\) 0.05 \({}^{\mathrm{d}}\) & 41,900 \(\pm\) 1870 \({}^  
\begin{table}
\begin{tabular}{c c c c c} \hline \hline  & \multicolumn{2}{c}{**Dealcohilized and Natural Wines**} & \multicolumn{2}{c}{**Reconstituted Wines**} \\ \cline{2-5}
**Compound (\(\mathbf{ug\,L^{-1}}\))** & \multicolumn{2}{c}{**5\% v/v**} & \multicolumn{2}{c}{**8\% v/v**} & \multicolumn{2}{c}{**12.2\% v/v**} & \multicolumn{2}{c}{**24 h**} & \multicolumn{2}{c}{**14 Days**} \\ \hline ethyl butyrate & 0.96 \(\pm\) 0.01\({}^{\,\mathrm{a}}\) & 2.42 \(\pm\) 0.23\({}^{\,\mathrm{b}}\) & 4.12 \(\pm\) 0.05\({}^{\,\mathrm{c}}\) & 0.87 \(\pm\) 0.02\({}^{\,\mathrm{a}}\) & 0.88 \(\pm\) 0.01\({}^{\,\mathrm{a}}\) \\ ethyl-2-methyl butyrate & 0.11 \(\pm\) 0.00\({}^{\,\mathrm{b}}\) & 0.20 \(\pm\) 0.01\({}^{\,\mathrm{c}}\) & 0.25 \(\pm\) 0.00\({}^{\,\mathrm{d}}\) & 0.09 \(\pm\) 0.00\({}^{\,\mathrm{b}}\) & 0.07 \(\pm\) 0.00\({}^{\,\mathrm{a}}\) \\ ethyl-3-methyl butyrate & 0.20 \(\pm\) 0.00\({}^{\,\mathrm{c}}\) & 0.04 \(\pm\) 0.01\({}^{\,\mathrm{b}}\) & 0.05 \(\pm\) 0.00\({}^{\,\mathrm{b}}\) & 0.02 \(\pm\) 0.00\({}^{\,\mathrm{a}}\) & 0.01 \(\pm\) 0.00\({}^{\,\mathrm{a}}\) \\ isoamyl acetate & 0.04 \(\pm\) 0.01\({}^{\,\mathrm{a}}\) & 0.23 \(\pm\) 0.1\({}^{\,\mathrm{b}}\) & 0.21 \(\pm\) 0.00\({}^{\,\mathrm{b}}\) & BLQ & \\
3-methyl-1-butanol & 3.92 \(\pm\) 0.04\({}^{\,\mathrm{ab}}\) & 5.56 \(\pm\) 0.26\({}^{\,\mathrm{b}}\) & 6.68 \(\pm\) 0.74\({}^{\,\mathrm{c}}\) & 2.78 \(\pm\) 0.11\({}^{\,\mathrm{a}}\) & 2.92 \(\pm\) 0.07\({}^{\,\mathrm{a}}\) \\ ethyl hexanoate & 3.79 \(\pm\) 0.04\({}^{\,\mathrm{ab}}\) & 5.77 \(\pm\) 0.70\({}^{\,\mathrm{b}}\) & 11.6 \(\pm\) 0.33\({}^{\,\mathrm{c}}\) & 2.62 \(\pm\) 0.12\({}^{\,\mathrm{a}}\) & 2.84 \(\pm\) 0.03\({}^{\,\mathrm{a}}\) \\ ethyl-3-lactate & 4.799 \(\pm\) 0.80\({}^{\,\mathrm{ab}}\) & 5.230 \(\pm\) 5.20\({}^{\,\mathrm{b}}\) & 6.650 \(\pm\) 9.90\({}^{\,\mathrm{b}}\) & 4.360 \(\pm\) 2.00\({}^{\,\mathrm{a}}\) & 4.280 \(\pm\) 1.30\({}^{\,\mathrm{a}}\) \\ (z)-3-hexened & 3.75 \(\pm\) 0.06\({}^{\,\mathrm{c}}\) & 4.08 \(\pm\) 0.03\({}^{\,\mathrm{c}}\) & 4.68 \(\pm\) 0.14\({}^{\,\mathrm{d}}\) & 2.35 \(\pm\) 0.07\({}^{\,\mathrm{a}}\) & 3.07 \(\pm\) 0.03\({}^{\,\mathrm{b}}\) \\ methyl octanoate & 0.01 \(\pm\) 0.00 & 0.01 \(\pm\) 0.00 & 0.03 \(\pm\) 0.00 & BLQ & BLQ \\ ethyl octanoate & 8.82 \(\pm\) 0.09\({}^{\,\mathrm{b}}\) & 15.55 \(\pm\) 0.71\({}^{\,\mathrm{a}}\) & 32.35 \(\pm\) 0.65\({}^{\,\mathrm{d}}\) & 5.23 \(\pm\) 0.02\({}^{\,\mathrm{a}}\) & 5.82 \(\pm\) 0.07\({}^{\,\mathrm{a}}\) \\ propononic acid & 200 \(\pm\) 200 & 230 \(\pm\) 10 & 180 \(\pm\) 0.00 & 180 \(\pm\) 0.00 & 210 \(\pm\) 0.00 \\ linalool & 0.29 \(\pm\) 0.00\({}^{\,\mathrm{c}}\) & 0.37 \(\pm\) 0.00\({}^{\,\mathrm{d}}\) & 0.08 \(\pm\) 0.00\({}^{\,\mathrm{b}}\) & 0.05 \(\pm\) 0.00\({}^{\,\mathrm{a}}\) & 0.06 \(\pm\) 0.00\({}^{\,\mathrm{a}}\) \\ methyl decanoate & BLQ & 0.01 \(\pm\) 0.00 & 0.02 \(\pm\) 0.00 & 0.01 \(\pm\) 0.00 & 0.01 \(\pm\) 0.00 \\ ethyl decanoate & 4.18 \(\pm\) 0.06\({}^{\,\mathrm{b}}\) & 3.59 \(\pm\) 0.31\({}^{\,\mathrm{b}}\) & 12.42 \(\pm\) 0.33\({}^{\,\mathrm{c}}\) & 0.87 \(\pm\) 0.42\({}^{\,\mathrm{a}}\) & 1.82 \(\pm\) 0.03\({}^{\,\mathrm{a}}\) \\ isoamyl octanoate & 0.04 \(\pm\) 0.00\({}^{\,\mathrm{b}}\) & 0.03 \(\pm\) 0.00\({}^{\,\mathrm{b}}\) & 0.06 \(\pm\) 0.00\({}^{\,\mathrm{c}}\) & 0.01 \(\pm\) 0.00\({}^{\,\mathrm{a}}\) & 0.01 \(\pm\) 0.00\({}^{\,\mathrm{a}}\) \\ 3-(methylthio-1-propanol) & 4.09 \(\pm\) 0.22\({}^{\,\mathrm{b}}\) & 4.33 \(\pm\) 1.76\({}^{\,\mathrm{b}}\) & 38.24 \(\pm\) 1.46\({}^{\,\mathrm{b}}\) & 2.91 \(\pm\) 1.61\({}^{\,\mathrm{a}}\) & 40.44 \(\pm\) 1.27\({}^{\,\mathrm{b}}\) \\ \(\beta\)-phenyl ethyl acetate & 0.03 \(\pm\) 0.00\({}^{\,\mathrm{ab}}\) & 0.04 \(\pm\) 0.00\({}^{\,\mathrm{b}}\) & 0.04 \(\pm\) 0.00\({}^{\,\mathrm{c}}\) & 0.03 \(\pm\) 0.00\({}^{\,\mathrm{a}}\) & 0.03 \(\pm\) 0.00\({}^{\,\mathrm{a}}\) \\ ethyl dodecanoate & 0.06 \(\pm\) 0.00\({}^{\,\mathrm{b}}\) & 0.07 \(\pm\) 0.00\({}^{\,\mathrm{b}}\) & 0.3 \(\pm\) 0.01\({}^{\,\mathrm{c}}\) & 0.02 \(\pm\) 0.00\({}^{\,\mathrm{a}}\) & 0.02 \(\pm\) 0.00\({}^{\,\mathrm{a}}\) \\ geraniol & 0.20 \(\pm\) 0.00\({}^{\,\mathrm{c}