## 4 Discussion  
The objective of our study was to assess the level of postoperative pain experienced following two distinct treatment protocols (LANAP and OHLLT) utilizing laser technology for the management of patients with periodontitis. Furthermore, the effectiveness of both LANAP and OHLLT methods is confirmed using real-time PCR microbiological investigation. This approach enables the precise and quantitative identification of significant indicators associated with periodontitis and peri-implantitis while also facilitating the assessment of the overall bacterial load [32]. This topic holds significance, especially when considering the existing literature that discusses the presence of periodonopathogenic bacteria in the periodontal pockets and alveoli of the maxilla and mandible. It is particularly relevant to explore the effectiveness of periodontal surgery and nonsurgical procedures in addressing this issue. The persistence of some submucosal marginal bacterial species, including _A. actinocytencomitus_, _P. gingivalis_, and _T. denticola_ (the ability to penetrate adjacent periodontal tissues), is related to poor response to treatment with scaling and root canal therapy. Therefore, it is not recommended to employ nonsurgical periodontal techniques when _Actinobacillus actynomicetemontains_, _Porphyomonas gingivalis_, _Prerotella intermedia_, and _Campylobacter rectus_ are detected in the periodontal pocket [1]. The regenerative surgical approach yielded comparable outcomes, as demonstrated by the findings of [PERSON] and [PERSON] [2]. Their study revealed that the presence of elevated bacterial levels and specific pathogenic complexes in the periodontal pockets surrounding the implants had a notable and adverse impact on the one-year prognosis of both surgical and regenerative interventions. If the existence of red and orange Sokransky complexes exerts an adverse influence on both nonsurgical and surgical interventions for periodontal disease, it is advisable to employ real-time PCR whenever treatment of impacted teeth becomes necessary. [PERSON] et al. [35] conducted a recent meta-analysis that adhered to the PRISMA standards [33] and the Cochrane Handbook for Systematic Reviews of Intervention [36]. The study focused on the in vivo therapy of peri-implantitis utilizing laser treatments. The evaluation process involved the examination of publications that had been published  
Figure 8: 1064 nm Nd:YAG laser radiation (LANAP). Figure 9: OHLLT and LANAP protocol resumes, according to previous research [7; 9; 11; 30; 31; 32; 33; 34]. in vivo. The researchers analyzed a total of 49 papers; however, only seven articles met the rigorous qualifying criteria for inclusion in the quality evaluation. The present analysis incorporated a total of seven investigations, namely [PERSON] et al. [37], [PERSON] et al. [38], [PERSON] et al. [39], [PERSON] et al. [40], [PERSON] et al. [41], and [PERSON] et al. [36]. The researchers concluded that high-power diode lasers might exhibit an impact on peri-implant microorganisms, hence potentially contributing to the development of peri-implantitis. Antimicrobial photodynamic therapy (aPDT) demonstrates efficacy in reducing the overall prevalence of several bacterial strains commonly associated with peri-implantitis, such as _A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. donticola, T. forythia, F. nuclatum_, and _C. rectus_. Consequently, the authors' findings align precisely with the conclusions presented in the publication by [PERSON] et al. in 2016 [42]. Real-time polymerase chain reaction (PCR) methodologies enable the assessment and juxtaposition of microbiological tests pre- and post-treatment utilizing two distinct methods. A positive link has been seen between the severity of periodontitis and the overall quantity of bacteria present in periodontal pockets [1; 2; 32]. In summary, it can be asserted that the proportions of _P. gingivalis_, _T. forythia_, and _T. forythia_ were comparable in the sample groups (groups 1 and a) following one week of therapy.