## 1 Introduction  
In recent years, the constant development of wound management techniques and dressings to overcome chronic wounds and enable the effective treatment of wounds associated with hospital infections or burn wounds has been observed [1; 2; 3]. The trend is shown in Figure 1. Such a demand for research is due to the increasing bacterial resistance to antibiotics worldwide, due to several contributing factors, including inappropriate antibiotic utilization in clinics and widespread use in the food industry [4]. Drug-resistant bacteria and the associated infections result in high morbidity and mortality rates [5; 6]. _P. aeruginosa_ is one of the leading causes of hospital-acquired infections, which also justifies the increase in scientific papers each year focusing on the multi-drug resistance of this microorganism (Figure 1). Recent studies indicate an increasing variety of virulence factors (especially toxins) in _P. aeruginosa_ that is becoming a major concern for clinicians, while _P. aeruginosa_ has a higher chance of infecting immunocompromised hosts [7; 8]. One of the solutions is to perform preventive procedures for the control of infections with multidrug-resistant microorganisms, e.g., removal of sinks in intensive care units, better processing of waste water, etc. [9]. However, such solutions do not solve the problem completely, but reduce the number of incidents. Therefore, the interest in the development of effective methods and approaches to treat bacterial infections is high and the topic remains very relevant [10]. The chemical antimicrobial compounds used to treat wounds are usually limited by their specific mechanism of bio-interaction, while extensive burn injuries are challenging in terms of clinical diagnosis and the rapid definition of effective antimicrobial therapy [11]. Therefore, novel techniques have to be proposed to address the multi-drug resistance of pathogenic and opportunistic microorganisms [12]. Ultimately, the approach should be universal and non-specific to a certain microorganism. One of these approaches is the use of acetic acid (AA), which is heavily employed in hospitals to treat burn infections. The AA is applied topically within dressings and is well tolerated by patients in small concentrations; however, with higher concentrations (e.g., 5%), patients complain of stinging and pain on application of acetic acid to wounds [13; 14]. Nevertheless, it does not encourage the evolution of multiple drug-resistant strains of microorganisms in hospital environments; therefore, this method remains relevant for thousands of years [15]. Another solution is to use physical methods such as photodynamic therapy or cold plasma treatment. In the case of photodynamic therapy, frequently, an additional photosensitizer has to be employed [16]. The direct exposure to plasma terminates the infection by destroying the biofilm over it; therefore, it is considered a good treatment option for wound healing [17]. The potential of pulsed electric fields (PEF) in this context has been also highlighted recently [18; 19; 20]. Subjecting cells to high-intensity PEF results in a phenomenon known as electroporation [21]. The cells are polarized in an electric field, and when a certain threshold transmembrane potential is reached, hydrophilic pores are formed in the membrane, enabling effective mass transfer [22]. If the intensity is further increased (amplitude, number of pulses or duration), the process becomes irreversible and the cells die (i.e., irreversible electroporation). This phenomenon is heavily used and is not limited to food processing as a non-thermal sterilization method [23]. In vitro data suggest the promising combination of PEF with antimicrobials, enabling the sensitization of microorganisms to chemical treatment [24; 25; 26; 27]. Recently, our group has presented a proof of concept regarding the application of PEF with AA, which results in the effective killing of bacteria on the skin surface [5].