## 2 Results and Discussion
### Analysis and Quantification of Atropine and Scopolamine in Different Types of Infusions  
A total of 33 samples were studied using the \(\upmu\)SPEDed(r) extraction procedure followed by HPLC-MS/MS, as shown in Figure 1a. Matrix-matched calibration curves with internal standards were used for quantification (see Section 4.6) of the Tea-B-3 and Her-Inf-4 samples. To achieve this, 12.5 \(\upmu\)L of 500 ng/mL of an internal standard solution containing (\(\pm\))-atropine-D3 and (\(-\))-scopolamine-D3 was added to 2.5 mL of each filtered infusion before the \(\upmu\)SPEDed(r) extraction procedure. The extracts were then analyzed using the HPLC-MS/MS system (Figure 1), and quantification was performed using either the Tea-B-3 calibration curve (for the Tea-B-1-Tea-B-8 samples) or the Her-Inf-4 calibration curve (for the Her-Inf-1-Her-Inf-17 samples). Extracted ion chromatograms (quantification ion) of \begin{table}
\begin{tabular}{c c c} \hline \hline
**Infusion Code** & \
\begin{tabular}{c} **(\(\pm\))-Atropine-D3** \\ **(Recovery \% \(\pm\) SD)** \\ \end{tabular} &
\begin{tabular}{c} **(\(-\))-Scopolamine-D3** \\ **(Recovery \% \(\pm\) SD)** \\ \end{tabular} \\ \hline Tea-W-1 & \(101\pm 3\) & \(98\pm 8\) \\ Tea-W-2 & \(103\pm 4\) & \(101\pm 5\) \\ Tea-W-3 & \(89\pm 9\) & \(81\pm 9\) \\ Tea-G-1 & \(102\pm 1\) & \(95\pm 2\) \\ Tea-G-2 & \(102\pm 1\) & \(105\pm 3\) \\ Tea-G-3 & \(97\pm 6\) & \(103\pm 1\) \\ Tea-G-4 & \(106\pm 5\) & \(102\pm 2\) \\ Tea-G-5 & \(97\pm 1\) & \(102\pm 2\) \\ Tea-B-1 & \(97\pm 1\) & \(100\pm 1\) \\ Tea-B-2 & \(104\pm 1\) & \(106\pm 4\) \\ Tea-B-3 & \(107\pm 1\) & \(103\pm 5\) \\ Tea-B-4 & \(101\pm 3\) & \(99\pm 7\) \\ Tea-B-5 & \(96\pm 5\) & \(89\pm 3\) \\ Tea-B-6 & \(102\pm 3\) & \(94\pm 9\) \\ Tea-B-7 & \(99\pm 1\) & \(90\pm 1\) \\ Tea-B-8 & \(105\pm 4\) & \(92\pm 2\) \\ Her-Inf-1 & \(103\pm 8\) & \(73\pm 3\) \\ Her-Inf-2 & \(102\pm 5\) & \(99\pm 3\) \\ Her-Inf-3 & \(97\pm 4\) & \(104\pm 2\) \\ Her-Inf-4 & \(98\pm 4\) & \(86\pm 9\) \\ Her-Inf-5 & \(106\pm 1\) & \(97\pm 4\) \\ Her-Inf-6 & \(104\pm 9\) & \(103\pm 11\) \\ Her-Inf-7 & \(108\pm 8\) & \(114\pm 2\) \\ Her-Inf-8 & \(106\pm 5\) & \(102\pm 4\) \\ Her-Inf-9 & \(103\pm 1\) & \(73\pm 7\) \\ Herb-Inf-10 & \(101\pm 1\) & \(87\pm 1\) \\ Herb-Inf-11 & \(102\pm 1\) & \(98\pm 1\) \\ Herb-Inf-12 & \(103\pm 6\) & \(106\pm 4\) \\ Herb-Inf-13 & \(98\pm 1\) & \(85\pm 3\) \\ Herb-Inf-14 & \(105\pm 1\) & \(92\pm 6\) \\ Herb-Inf-15 & \(109\pm 9\) & \(96\pm 1\) \\ Herb-Inf-16 & \(92\pm 8\) & \(93\pm 8\) \\ Herb-Inf-17 & \(93\pm 3\) & \(96\pm 3\) \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Recovery percentages (\(n=3\)) evaluated for all infusion samples studied at a concentration of 2.5 ng/mL. a sample of white tea (Tea-W-1) infusion contaminated with TAs and an uncontaminated infusion sample of echinacea (Herb-Inf-5), both compared with a standard solution of 1 ng/mL, are shown in Figure 1b,c, respectively. The results of the sample analysis are presented in Table 3. Of the 33 samples tested (Table S1), 21 were contaminated with one or both toxins, corresponding to 64% of the infusion samples studied. In the case of teas, these samples were the most contaminated (Table 3), specifically green and white tea infusions, which generally contain both analytes, whereas black tea infusions were only contaminated with atropine. However, all tea samples exceeded the Commission Regulation (EU) 2021/1408 value (0.2 ng/mL in liquid herbal infusions) [29]. Teas are obtained from the dried leaves of a small tree called _Camellia sinensis_ (L.) [PERSON], which belongs to the family Theaceae, D. [PERSON] (Figure 2). The different types of teas (white, green, and black) are due to the harvesting and processing steps involved in the tea manufacturing process. Leaves can be collected manually or automatically in all cases [35]. White tea is picked before green and black tea, and buds are picked when they are not open.