## 4 Conclusions  
A simultaneous HPLC analysis method for quality assessment of DHGST, a traditional herbal medicine prescription that has been used for arthritis and neuritis for a long time, was developed. The method was validated with respect to linearity, LOD, LOQ, recovery, and precision. As a result of these investigations, components of _G. strantinea, E. ulmoides_, and _P. lactiflora_ in the DHGST sample, such as gentiopicroside (**6**, 14.29-14.68 mg/g), pinoresinol diglucoside (**7**, 9.69-9.81 mg/g), and paeoniflorin (**10**, 4.68-4.77 mg/g), were found in the highest amounts. The developed and validated method is suitable for use in the quality assessment of DHGST or other herbal medicine prescriptions.  
The following are available online at [[https://www.mdpi.com/article/10.3390/app11177829/s1](https://www.mdpi.com/article/10.3390/app11177829/s1)]([https://www.mdpi.com/article/10.3390/app11177829/s1](https://www.mdpi.com/article/10.3390/app11177829/s1)), Figure S1: Chemical structures of the 24 marker components in DHGST; Figure S2: HPLC chromatogram of the herbal medicine extract and its main components. (A): _A. continentalis_; (B): _A. sigas_; (C), _P. lactiflora_; (D), _T. clinensis_; (E), _R. glutinosa_; (F), _C. officinal_; (G), _P. ginseng_; (H), _P. oxcos_; (I), _A. bidentata_; (D), _E. ulmoides_; (K), _G. stramine_; (L), _A. heterotropoides_; (M), _S. divarate_; (N), _C. cassia_; (O), _G. uralensis_; and (P), _Z. officinale_; Figure S3: HPLC chromatograms of the standard solution (A) and 70% methanol of DHGST sample (B). Gallic acid (**1**), 5-hydroxymethylfurfurral (**2**), geniposidic acid (**3**), loganic acid (**4**), cimufugin (**5**), chlorogenic acid (**6**), geniposide (**7**), gentiopicroside (**8**), pinoresinol diglucoside (**9**), albiflorin (**10**), _prim-O_-glucosylclimifugin (**11**), paeoniflorin (**12**), genipin (**13**), lequiritin apioside (**14**), liquiritin (**15**), edcystore (**16**), ferulic acid (**17**), nodakenin (**18**), 5-O-methylismmidoside (**19**), quercitrin (**10**), benzoic acid (**21**), ginnesnoglic Rg1 (**22**), cumararin (**23**), cinnamic acid (**24**), benzoyloeniflorin (**25**), ginsnoside Rb1 (**26**), cinnaldehyde (**27**), glycyrrhizin (**28**), 6-gingerol (**29**), aristololcic acid II (**30**), aristolcic acid I (**31**), \(\beta\)-asarone (**32**), methyleugenol (**33**), \(\alpha\)-asarone (**34**), safrole (**35**), decursinol angelate (**36**), decursinol angelate (**37**), pacynamic acid (**38**), continentalic acid (**39**), and kaureonic acid (**40**); Table S1: Information and composition of DHGST; Table S2: HPLC operating conditions for simultaneous quantification of the 24 marker components in DHGST; Table S3: System suitability for the analysis of the 24 marker compounds with the developed HPLC method; Table S4: Repeatability of retention time of the 24 marker analytes using HPLC (\(n=6\)); Table S5: Repeatability of peak area of the 24 marker analytes by HPLC (\(n=6\)).  
Conceptualization, C-S.S. and H.-K.S.; performing experiments, analyzing data, and writing--original draft preparation, C-S.S.; funding acquisition, H.-K.S. All authors have read and agreed to the published version of the manuscript.  
This research was supported by grants from the Korea Institute of Oriental Medicine (grant numbers KSN2013310 and KSN2021310).  
Not applicable.  
The authors have declared no conflict of interest.