## 3 Results
### Bacterial Growth in Healthy Sheep  
Bacterial growth was obtained in the twelve samples collected from the gingival margins of periodontally healthy sheep analysed. All the cultures obtained were mixed, except in two animals, isolated _Streptococcus bovis_ in pure culture (2/12: 16.66%). In total, 91 different isolates were obtained from healthy mouths. Table 2 shows the isolated microorganisms and their relative importance in relation to the total number of isolates (91). \begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Microorganisms** & **Pure** & **Pure Cultures (\%)** & **Total** & **Within Isolates (\%)** \\ \hline \(G^{o}\)_Streptococcus_ & 17 & 26.56\% & 101 & 20.95\% \\ _Streptococcus suis_ & 0 & 0.00\% & 5 & 1.04\% \\ _Streptococcus bovis_ & 0 & 0.00\% & 4 & 0.83\% \\ _G\({}^{o}\)Pseudomonas_ & 9 & 14.06\% & 41 & 8.50\% \\ _Pseudomonas fluorescens_ & 0 & 0.00\% & 3 & 0.62\% \\ _Pseudomonas alcaligenes_ & 0 & 0.00\% & 2 & 0.41\% \\ _Pseudomonas luteola_ & 0 & 0.00\% & 1 & 0.21\% \\ _Pseudomonas putida_ & 0 & 0.00\% & 1 & 0.21\% \\ _Trueperella pyogenes_ & 6 & 9.37\% & 35 & 7.26\% \\ _G\({}^{o}\)Staphylococcus_ & 2 & 3.13\% & 32 & 6.63\% \\ _Staphylococcus aureus_ & 1 & 1.56\% & 6 & 1.24\% \\ _Escherichia coli_ & 7 & 10.93\% & 30 & 6.22\% \\ _Fusobacterium mecrophorum_ & 0 & 0.00\% & 27 & 5.60\% \\ _G\({}^{o}\) Bacillus_ & 3 & 4.68\% & 27 & 5.60\% \\ _G\({}^{o}\) Moraxella_ & 1 & 1.56\% & 22 & 4.56\% \\ _G\({}^{o}\) Microoccus_ & 1 & 1.56\% & 21 & 4.36\% \\ _G\({}^{o}\) Enterobacter_ & 6 & 9.37\% & 16 & 3.32\% \\ _G\({}^{o}\) Klebsiella_ & 6 & 9.37\% & 14 & 2.90\% \\ _Manuheimia haemolytica_ & 4 & 6.25\% & 14 & 2.90\% \\ _Bacteroides fragilis_ & 0 & 0.00\% & 13 & 2.70\% \\ _G\({}^{o}\) Neisseria_ & 0 & 0.00\% & 13 & 2.70\% \\ _G\({}^{o}\) Acinetobacter_ & 0 & 0.00\% & 12 & 2.49\% \\ _G\({}^{o}\) Actinomyces_ & 0 & 0.00\% & 8 & 1.66\% \\ _G\({}^{o}\) Corynebacterium_ & 1 & 1.56\% & 9 & 1.86\% \\ _Corynebacterium urealyticum_ & 0 & 0.00\% & 1 & 0.21\% \\ _G\({}^{o}\) Prevotella_ & 0 & 0.00\% & 8 & 1.66\% \\ _G\({}^{o}\) Proteus_ & 1 & 1.56\% & 7 & 1.45\% \\ _Bacteroides capillosus_ & 0 & 0.00\% & 5 & 1.04\% \\ _Aclaligenes faecalis_ & 0 & 0.00\% & 3 & 0.62\% \\ _Salmonella enterica sub diarrhoea_ & 0 & 0.00\% & 3 & 0.62\% \\ _Aerococcus urinane_ & 0 & 0.00\% & 2 & 0.41\% \\ _Aerococcus viridans_ & 0 & 0.00\% & 2 & 0.41\% \\ _Bacteroides urealyticus_ & 0 & 0.00\% & 2 & 0.41\% \\ _Bibersteinia trehalosi_ & 0 & 0.00\% & 2 & 0.41\% \\ _Cellulostimicrophum cululans_ & 0 & 0.00\% & 2 & 0.41\% \\ _Fusobacterium nucleatum_ & 0 & 0.00\% & 2 & 0.41\% \\ _Pasteuella multicola_ & 0 & 0.00\% & 2 & 0.41\% \\ _G\({}^{o}\) Aeromonas_ & 0 & 0.00\% & 1 & 0.21\% \\ _G\({}^{o}\) Dermabacter_ & 0 & 0.00\% & 1 & 0.21\% \\ _G\({}^{o}\) Porphymomonas_ & 0 & 0.00\% & 1 & 0.21\% \\ _G\({}^{o}\) Rhodococcus_ & 0 & 0.00\% & 1 & 0.21\% \\ _G\({}^{o}\) Serratia_ & 0 & 0.00\% & 1 & 0.21\% \\ _Prevotella denticola_ & 0 & 0.00\% & 1 & 0.21\% \\ Yeast & 0 & 0.00\% & 1 & 0.21\% \\ \hline Total & 64 & & 482 & \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Isolated microorganisms ordered by their relevance within the number of isolates (482) and the percentage of pure cultures. Microorganisms of genus _Streptococcus_ were the most frequently isolated (15/91: 16.48%), followed by bacteria of genus _Staphylococcus_ (12/91: 13.18%), _Pseudomonas_ (9/91: 9.89%) and _Moraxella_ (6/91: 6.59%).