## 3 Results and Discussions
### Microorganisms Related to Verticillium Wilt of Horseradish Roots
\begin{table}
\begin{tabular}{c c c c c c c c} \hline
**Protection** & \multicolumn{3}{c}{_D\(\widehat{\text{SI}}\)=\(\boldsymbol{b_{0}}\)+\(\boldsymbol{b_{1}}\)\(\cdot\)SP_} & \multicolumn{3}{c}{_D\(\widehat{\text{SI}}\)=\(\boldsymbol{a_{0}}\)+\(\boldsymbol{a_{1}}\)\(\cdot\)CFU_} & \multicolumn{1}{c}{_\(\boldsymbol{r_{\text{SP}}\)\(\cdot\)CFU_}} \\ \cline{2-9}
**Treatments** & \(\boldsymbol{b_{0}}\) & \(\boldsymbol{b_{1}}\) & \(\boldsymbol{r_{\text{DSI}}\)SP_} & \(\boldsymbol{a_{0}}\) & \(\boldsymbol{a_{1}}\) & \(\boldsymbol{r_{\text{DSI}}\)\(\cdot\)CFU_} & \\ \hline Control & 92.9 & \(-\)0.345 *** & \(-\)0.984 *** & 89.2 & \(-\)0.274 *** & \(-\)0.851 *** & 0.927 *** \\ ChP & 57.5 & \(-\)0.073 *** & \(-\)0.935 *** & 64.5 & \(-\)0.307 *** & \(-\)0.934 *** & 1.000 *** \\ RChP & 40.4 & \(-\)0.040 *** & \(-\)0.976 *** & 50.9 & \(-\)0.187 *** & \(-\)0.984 *** & 0.999 *** \\ BPPo & 76.4 & \(-\)0.101 *** & \(-\)0.895 *** & 93.7 & \(-\)0.302 *** & \(-\)0.996 *** & 0.928 *** \\ BPBs & 79.3 & \(-\)0.102 *** & \(-\)0.926 *** & 84.9 & \(-\)0.264 *** & \(-\)0.960 *** & 0.995 *** \\ BPEm & 35.0 & \(-\)0.024 *** & \(-\)0.712 *** & 30.0 & \(-\)0.029 & \(-\)0.205 & 0.830*** \\ \hline \end{tabular} \(t\)-test significant respectively at _p\(<\)_0.01 *;_p\(<\)_0.001 ***. Control - Without protection; ChP—Chemical protection; RChP—Reduced chemical protection with the participation of higherpations and biostimulus; BPPo—Biological protection (root cuttings treatment before planting with _Bacillus subtilis_); BPEm—Biological protection (root cuttings treatment before planting with _Bacillus subtilis_); BPEm—Biological protection (root cuttings treatment before planting with _Ecklmian maxim_).  
\end{table} 
Table 3: Relationships between the intensity of Verticillium wilt of horseradish roots (DSI) and the number of colony forming units (CFUs) and between the intensity of Verticillium wilt of horseradish roots (DSI) and the sum of precipitation in the growing season (SP) and the CFUs and the sum of precipitation in the growing season. Figure 2: Number of colony forming units (CFUs) isolated from internally infected horseradish roots. For all protective treatments, the coefficients of the linear regression equations of the intensity of the DSI of Verticillium wilt of horseradish roots from the sum of atmospheric precipitation during the growing season (SP) are statistically significant (Table 3). As the amount of rainfall increases, the DSI value decreases. For the BPEm treatment, an increase in precipitation by 1 mm causes the smallest (0.024 pp) reduction in the severity of this disease. In this combination, no statistically significant relationship was found between DSI Verticillium wilt of horseradish roots and CFUs. These relationships were statistically significant with respect to the remaining treatments. For ChP, an increase in CFU by one unit causes a DSI decrease of 0.307 pp. There was a significant positive correlation between the amount of precipitation during the growing season and CFUs. 2.2 Qualitative Analysis of Microorganisms Colonizing Horseradish Roots Affected by Verticillium Wilt  
In the course of the pathogenesis of Verticillium wilt of horseradish root, the species composition, frequency, and biodiversity of the microorganisms involved are important. During the three-year study; 3508 isolates were isolated from horseradish roots with clear symptoms of the disease (Table 4).