## 3 Results
### Donax Trunculus  
In contrast from what was observed with _C. gallina_, there was acute mortality of individuals of _D. trunculus_ during the experiments (Figure 5). Overall, the most notable decrease in percentage of survival was associated with the treatments conducted at the highest temperature, with a 100% mortality of individuals after 14 days at 27.5 \({}^{\circ}\)C/27-28 salinity (Figure 5c). The survival rate of the organism in the other treatments (low and medium temperatures and at all three salinity levels) ranged between 70 and 90% (Figure 5a,b).  
There was a significant difference in LMS (expressed as NRRT and %LMS) as a result of both temperature and salinity, and there was a significant interaction between these factors (2-way ANOVA; \(p\)-value \(<\) 0.05) (Table 2). LMS in _D. trunculus_ hemocytes (expressed as mean value of %LMS) was significantly lower in survival clams from the treatments conducted at 27.5 \({}^{\circ}\)C than in the other treatments (Figure 6). The highest mean values of LMS were observed in clams from the 20 \({}^{\circ}\)C/32-33 of salinity (NRRT = 165.9 \(\pm\) 10.7 min; %LMS = 82.7 \(\pm\) 5.1) and 20 \({}^{\circ}\)C/37-38 of salinity (NRRT = 136.9 \(\pm\) 13.1 min; % LMS = 72.8 \(\pm\) 6.7) treatments (Table S1).  
Pairwise comparisons revealed the existence of LMS response subsets (6 subsets for NRRT and 4 subsets for %LMS) ([PERSON]'s post hoc test; \(p\)-value \(<\) 0.05) (Figure 6a,b, Tables S6 and S7). The treatments carried out at the same temperature but at different salinities had similar LMS results in %LMS (Figure 6b, Tables S6 and S7) ([PERSON]'s post hoc test; \(p\)-values \(>\) 0.05).  
There was a significant difference in condition index due to temperature, but there was no effect of salinity or the interaction of both factors (Table 2). Unlike _C. gallina_, the CI of _D. trunculus_ decreased with increasing temperature (Table 3). Organisms exposed to a low temperature exhibited the highest CI values, showing no significant differences due to salinity. At a medium temperature (20 \({}^{\circ}\)C), a lower CI was obtained compared to those exposed at a low temperature (12 \({}^{\circ}\)C). The reference CI (of 19.5 \({}^{\circ}\)C and 36.8 salinity) differed from the CI obtained at 27-28 and 32-33 (\(p\)-value = 0.007, Table 3). Finally, organisms exposed to a high temperature (27.5 \({}^{\circ}\)C) had the lowest CI values. At this temperature, significant differences were observed between the reference CI (23.5 \({}^{\circ}\)C and 37.1 salinity) and those exposed to salinities of 32-33 and 37-38 (\(p\)-value = 0.000, Table 3). It should be noted that, during exposure to a high temperature, spawning occurred in _D. trunculus_, coinciding with the low LMS and CI recorded, pointing to a temperature-induced spawning event in this species.  
Figure 4: Correlation between CI and lysosomal membrane stability (%) of each treatment, for both species. The different colors represent the low—(blue), medium—(green), and high—(red) temperature treatments.  
Correlation analysis also revealed positive correlations between the two \"endpoints\" (Table S8). In _D. trunculus_ (\(n=8\)), a negative correlation was observed between CI and treatment temperature ([PERSON]'s correlation Coeff. = \(-0.976\); \(p\)-value = 0.000) and a moderate positive correlation between CI and %LMS ([PERSON]'s correlation Coeff. = 0.737; \(p\)-value = 0.037) (Figure 4).  
Figure 5: Percentage of _Donax trunculus_’ survival as a function of salinity at each treatment temperature during 21 days of exposure at a (**a**) low water temperature (12 \({}^{\circ}\)C), (**b**) medium water temperature (20 \({}^{\circ}\)C), and (**c**) high water temperature (27.5 \({}^{\circ}\)C) at salinities of 27–28, 32–33, and 37–38, respectively.