## 2 Materials and Methods
### Starvation-Induced Cross-Resistance Challenges  
Cross-resistance challenges were performed as described by [PERSON] _et al._[32] with minor changes regarding the growth medium, dilution buffer, and challenge parameters. Overnight cultures were prepared by inoculating 1 mL of M9 HiC media with cells from a single, isolated colony on LB agar. Overnight cultures were incubated aerobically for 24 h at either 30 \({}^{\circ}\)C or 37 \({}^{\circ}\)C. The following day, 40 \(\upmu\)L of overnight culture was transferred to 4 mL of either M9 HiC or M9 LoC media (1:100 v/v dilution) and incubated aerobically by shaking at either 30 \({}^{\circ}\)C or 37 \({}^{\circ}\)C. Growth was monitored by taking optical density readings (OD\({}_{600}\)) every 30 min. Log-phase (LP) cells were from M9 HiC cultures which had reached an OD\({}_{600}\) of 0.3-0.4 (mid-log-phase; \(\sim\)10\({}^{8}\)-10\({}^{9}\) CFU/mL). In M9 LoC cultures, glucose is depleted and growth stops, after approximately 4-5 h of incubation, at an equivalent OD in the range of 0.3-0.4. Carbon-starved (CS) cells were from M9 LoC cultures, which were kept in the incubator for 24 h after growth had ceased (24-h starved cells).  
Challenge cultures were prepared by diluting 10 \(\upmu\)L of LP or CS cells in 990 \(\upmu\)L of M9 NoC media (M9 buffer) either pre-warmed to 50 \({}^{\circ}\)C, previously lowered to a pH of 2.8 by the addition of HCl, or containing 15 mM H\({\rm{O}}\)z. The challenge conditions chosen were a balance between the level of the stress and the exposure time which allowed for direct comparisons of the survival of LP and CS cells at different incubation temperatures. Thermal challenge cultures were incubated in a 50 \({}^{\circ}\)C water bath without shaking. Acid and H\({\rm{O}}\)z challenge cultures were incubated in a shaking incubator at either 30 \({}^{\circ}\)C or 37 \({}^{\circ}\)C. At the beginning of the challenge (time zero) and at later, pre-determined time points, 10-\(\upmu\)L samples were removed and serially diluted in microtiter plates containing 90 \(\upmu\)L of M9 buffer per well to generate a 10-fold dilution series. Ten-micoliters from each dilution was spotted onto LB agar in triplicate and plates were incubated at either 30 \({}^{\circ}\)C or 37 \({}^{\circ}\)C for 24 h. Colonies from suitable dilutions were counted and the number of CFU/mL in each challenge culture was determined. The percent survival relative to the initial CFU/mL for each challenge culture at time zero is shown.  
\begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Strain** & **Source of** & & & \\
**Designations** & **Isolation** & **Pigmentation** & **Other Relevant Characteristics** & **References** \\ \hline \multirow{4}{*}{ATCC 13880} & \multirow{4}{*}{Pond water} & Mixed colony & Type strain of species; quality & \\  & & pigmentation at 30 \({}^{\circ}\)C; less & control strain; genome & \\  & & pigmentation at 37 \({}^{\circ}\)C & sequence complete & \\ \multirow{4}{*}{Db10} & _Drosophila_ & No pigmentation at either & Invertebrate pathogen used in & \\  & _melanogaster_ & 30 \({}^{\circ}\)C or 37 \({}^{\circ}\)C & studies with host _Caenorhabditis_ & [30, 31] \\ \cline{1-1}  & & & _elegans_; genome sequence complete & \\ \cline{1-1}  & & Red pigmentation at & Positive for glucose fermentation & \\ \cline{1-1}  & & 30 \({}^{\circ}\)C; pale pink at 37 \({}^{\circ}\)C & and DNase activity; bile-resistant & This study \\ \hline \hline \end{tabular}
\end{table}
Table 1: Strains of _Serratia marcescens_ used in this study.