# Comparative Study of _Plantago media_ Extracts in the Treatment of _Acanthamoeba_ sp. Trophozoites
## 3 Results
\begin{table}
\begin{tabular}{c c c c c c} \hline \multirow{2}{*}{**IC\({}_{50}\)**} & _P. media_ **In-Vitro** & _P. media_ **In-Vitro** & _P. media_ & _P. media_ & _P. lanecolata_ \\  & **-Derived Shoots** & **-Derived Roots** & **Inflorescences** & **Leaves** & **Leaves** \\ \hline
2 nd day & 5.20 & 9.70 & 8.70 & 0.95 & 7.65 \\
3 rd day & 5.00 & 7.60 & 6.80 & 1.25 & 7.25 \\
4 th day & 3.30 & 5.30 & 6.30 & 1.80 & 0.55 \\ \hline \end{tabular}  
IC\({}_{50}\)—the half-maximum anti-amoebic inhibitory concentration.  
\end{table} 
Table 4: Determination of IC\({}_{50}\) [mg/mL] for the studied extracts of _P. media_ and _P. lanecolata_. \begin{table}
\begin{tabular}{c c c c c c c} \hline \multirow{2}{*}{**Plant Material**} & \multirow{2}{*}{**Extract**} & \multicolumn{6}{c}{**Duration of Treatment [Days]**} \\ \cline{3-8}  & & \multicolumn{2}{c}{**2 nd Day**} & \multicolumn{2}{c}{**3 rd Day**} & \multicolumn{2}{c}{**4 th Day**} \\  & & \multicolumn{2}{c}{**MN \(\pm\) SD**} & \multicolumn{1}{c}{**\% GI**} & \multicolumn{1}{c}{**MN \(\pm\) SD**} & \multicolumn{1}{c}{**\% GI**} & \multicolumn{1}{c}{**MN \(\pm\) SD**} & \multicolumn{1}{c}{**\% GI**} \\ \hline _Plantago media_ & control & 12.44 \(\pm\) 2.22 & 0 & 22.94 \(\pm\) 3.39 & 0 & 28.38 \(\pm\) 4.33 & 0 \\ in vitro shoots & 1 mg/mL & 12.18 \(\pm\) 2.77 & 2.10 & 18.41 \(\pm\) 4.31 & 19.75 & 21.82 \(\pm\) 6.80 & 23.11 \\  & 5 mg/mL & 6.33 \(\pm\) 1.98 * & 49.06 & 11.42 \(\pm\) 5.07 * & 50.22 & 10.25 \(\pm\) 3.47 * & 63.89 \\  & 10 mg/mL & 2.94 \(\pm\) 1.61 * & 76.27 & 4.20 \(\pm\) 2.14 * & 81.70 & 5.27 \(\pm\) 5.66 * & 81.44 \\ _Plantago media_ & control & 12.44 \(\pm\) 2.22 & 0 & 18.94 \(\pm\) 3.39 & 0 & 27.38 \(\pm\) 4.33 & 0 \\ in vitro roots & 1 mg/mL & 9.18 \(\pm\) 2.55 & 26.21 & 13.00 \(\pm\) 2.94 & 31.37 & 16.67 \(\pm\) 1.25 * & 39.12 \\  & 5 mg/mL & 8.83 \(\pm\) 1.67 & 29.02 & 10.25 \(\pm\) 3.49 * & 45.89 & 13.91 \(\pm\) 4.46 * & 49.20 \\  & 10 mg/mL & 6.03 \(\pm\) 1.91 * & 51.45 & 9.09 \(\pm\) 2.35 * & 52.01 & 12.00 \(\pm\) 2.52 * & 56.18 \\ _Plantago media_ & control & 12.12 \(\pm\) 2.82 & 0 & 26.44 \(\pm\) 6.04 & 0 & 28.00 \(\pm\) 5.35 & 0 \\ inflorescences & 1 mg/mL & 10.76 \(\pm\) 1.92 & 11.23 & 25.00 \(\pm\) 4.97 & 5.45 & 26.27 \(\pm\) 3.22 & 6.18 \\  & 5 mg/mL & 9.83 \(\pm\) 2.65 & 18.90 & 16.33 \(\pm\) 3.73 * & 38.24 & 16.42 \(\pm\) 4.35 * & 41.36 \\  & 10 mg/mL & 4.67 \(\pm\) 2.38 * & 61.47 & 8.00 \(\pm\) 2.47 * & 69.75 & 8.10 \(\pm\) 3.97 * & 71.04 \\ _Plantago media_ & control & 12.12 \(\pm\) 2.82 & 0 & 23.44 \(\pm\) 6.04 & 0 & 32.00 \(\pm\) 5.35 & 0 \\ leaves & 1 mg/mL & 6.00 \(\pm\) 1.97 * & 50.50 & 12.33 \(\pm\) 3.89 * & 47.40 & 17.67 \(\pm\) 5.03 * & 44.79 \\  & 5 mg/mL & 5.91 \(\pm\) 1.73 * & 51.24 & 10.25 \(\pm\) 3.92 * & 56.28 & 14.50 \(\pm\) 4.03 * & 54.69 \\  & 10 mg/mL & 5.05 \(\pm\) 2.39 * & 58.34 & 8.81 \(\pm\) 3.43 * & 62.42 & 12.40 \(\pm\) 3.88 * & 61.25 \\ _Plantago Lanccolata_ & control & 10.11 \(\pm\) 2.05 & 0 & 25.72 \(\pm\) 4.36 & 0 & 38.39 \(\pm\) 7.77 & 0 \\ leaves & 1 mg/mL & 9.76 \(\pm\) 2.60 & 3.47 & 18.67 \(\pm\) 4.88 & 27.42 & 14.00 \(\pm\) 5.18 * & 63.54 \\  & 5 mg/mL & 8.67 \(\pm\) 1.97 & 14.25 & 16.35 \(\pm\) 4.83 * & 26.57 & 10.25 \(\pm\) 3.47 * & 74.31 \\  & 10 mg/mL & 1.33 \(\pm\) 0.94 * & 86.85 & 3.51 \(\pm\) 2.32 * & 86.35 & 5.27 \(\pm\) 5.66 * & 86.27 \\ \hline \end{tabular}  
* \(P\) **< 0.05, statistically significant difference in comparison with the control during the same time interval; \(n\) = 18. MN—mean number of trophozoites, and GI—growth inhibition.  
\end{table} 
Table 3: Effect of extracts from _Plantago media_ biomass from in vitro culture (shoots and roots) and organs of plants from the cultivation on inhibition of _Acanthamoeba_ trophozoites during four-day treatment. In the case of the infloreserences extract, the dependence of the effect on the extract concentration and treatment time was noted.