## 4 Discussion
[PERSON] et al. [36] performed their extraction with AcOEt from the metabolic broth of endophytic fungi isolated from _Gynura procumhens_, using a time of 10 min, directly in the separation funnel. The best results for the extraction of phenolic compounds from the culture medium were with the species _Colletotrichum glossorioides_ SN11 (87 mg GAEq/g). In the same study, antioxidant activity was evaluated using the DPPH and FRAP methods, but the result was not directly proportional to the amount of total phenolics, and the extract produced by the species _Macrophomina phaseolina_ SN6 presented the best result for the sequestration of the DPPH radical, while the extract produced by _Phomopsis_ sp. SN2 showed the highest ferric reduction power. In another study, [PERSON] et al. [37] carried out extractions from the culture media of endophytic fungi isolated from _Schinus treehinthfolius_ Raddi using AcOEt, at 25 \({}^{\circ}\)C for 24 h, without agitation. The authors obtained 758.91 TEq mg/g (3.02 mmol TEq/g) in the extract produced by the species _Ochrocladosporium elatum_ for antioxidant activity using the DPPH radical sequestration method. In this case, it is observed that the longer extraction time favored the obtaining of phenolic compounds, contrary to what we observed for the extraction of phenolic metabolites from the culture medium of _C. manane_ CF2-13, whose best result (0.47 mmol TEq/g) was obtained with 2 h of extraction. On the other hand, after 24 h of the extraction of metabolites produced by the species _Penicillium olsonii_, [PERSON] et al. [37] obtained 119.33 mg TEq/g (0.47 mmol TEq/g), which is the same value obtained in our study with only 2 h of extraction with ethyl acetate under agitation. Therefore, the influence of the fungal species on the phenolic metabolic extraction process is clear, and it is necessary to study the optimal conditions for each species of fungus. Natural antioxidants produced by endophytic fungi, in general, are of the class of phenolic compounds, such as flavonoids and phenolic acids [38], which corroborates the results found in our study. The TLC results indicate the presence of these classes of molecules in the extract of _C. manane_ CF2-13 when obtained under the best extraction conditions (solvent AcOEt, at 20 \({}^{\circ}\)C for 2 h). [PERSON] et al. [39] also observed the presence of phenolic compounds and flavonoids in the metabolic extracts of the endophytic fungus _Aspergillus fumigatus_ isolated from _Moringa oleifera_, which exhibited pronounced antioxidant activity against DPPH radicals. On the other hand, in a recent study, [PERSON] et al. [29] identified not only phenolic compounds but also a new sesquiterpene in the extract of the endophytic fungi _Fusarium foetens_ AQF6, which presented pronounced antioxidant activity. Thus, these results confirm the potential of endophytic fungi as a source of natural antioxidant compounds, which can be phenolic compounds, terpen, and alkaloids, and consequently their possible use in the food industry. Phenolic acids have been found in the culture medium of endophytic fungi and are directly associated with the antioxidant activity of several fungal extracts. In the study by [PERSON] et al. [35], in which the composition of phenolic compounds in fungal extracts was evaluated using liquid chromatography coupled to mass spectrometry (LC-MS), caffe acid was found in the extracts of _P. oxalium_ and _Simplicillium_ sp., both obtained with AcOEt; and that of _Colletotrichum_ sp., obtained with butanol, at concentrations of 3.01, 0.52, and 0.12 umol/g, respectively. These results agree with those observed for the extract of _C. manane_ CF2-13, in which caffe acid was found at a concentration of 12.07 \(\mu\)g/mL (0.22 umol/g). In addition to caffe acid, [PERSON] et al. [35] also found chlorogenic acid in their fungal extracts, which was not observed in our research for _C. manane_ CF2-13. [PERSON] et al. [40] analyzed the extracts from the endophytic fungi isolated from _Alisma orientale_ ([PERSON].)