###### Abstract
Etomycorrhizal _Boletus edulis_ can decompose DDT, while _Gompidius vicsidus_, _Lacaria bicolor_, and _Leccinun scabrum_ can remove anthracene by biosorption [23]. Appropriately used mycorrhizal fungi can also reduce plant stress, e.g., in soils with high sodium contents and alkaline soils. Combining species that are tolerant of stress factors could be successful for ectomycorrhizal reclamation. Although they are mostly hydrophobic and lipophilic and are not easily taken up by plants, the presence of AMFs can enhance the accumulation of these pollutants in plant roots compared to plants without AMFs. In contrast, AMFs showed a pollutant immobilization ability in the roots, resulting in limited root-shoot transport of these pollutants [21]. The glomalin-related soil protein (GRSP) is a type of glycoprotein produced by AMFs in the phylum Glomeromycota. It is known for its contribution to the mitigation of soil degradation [24]. The GRSP improves nutrient management in soil and, therefore, plant productivity, soil organic carbon content, sequestration, and biological activity [25]. Glomalin also provides other plant and soil health benefits, including soil aggregation and enhancing the growth of plants under abiotic stress [26]. The GRSP can also affect the mobility of organic pollutants in the soil. The increasing release of PAHs from the soil particles followed by faster degradation of these pollutants and/or their better plant root uptake, as well as the opposite pattern resulting in decreased bioavailability of the pollutants, were observed as well [21]. The immobilization of risk elements by the GRSP via adsorption and complexation was also observed [27; 28; 29; 30]. The pollutant type and concentration, GRSP content, and the soil characteristics and plant species growing in these soils play an important role in these processes. [PERSON] et al. [30] confirmed the importance of the molecular weight of the GRSP in the potential interactions with PAHs. In this context, for the purpose of remediation, seedlings of trees and shrubs such as Pinaceae (_Pinus_), Salicaceae (_Salix_, _Populus_), Fagaceae (_Fagus_), and Betulaceae (_Betula_, _Alnus_) are planted, and bioaugmentation by the ectomycorrhizal fungi is provided [11]. The choice of the fungal species depends on the trees used, but it could be affected by the characteristics of the soil pollution. Frequently, the whole suite of pollutants can occur in extremely polluted areas, and a combination of species tolerant to various stress factors should be selected for potential bioremediation use. Therefore, indigenous species adaptedto the local climate and the long-lasting high pollution level should be preferred for the potential remediation of the site [11; 31; 32]. The city of Litvinov (North Bohemia, Czech Republic) is one of the most industrial cities in the Czech Republic, with a large petrochemical facility and intensive opencast brown coal mining area and coal processing. [PERSON] et al. [33] published a single study describing for the first time the pollution levels in the urban soils in Litvinov city. They also briefly summarized the history and current state of the industrial activities, resulting in the massive pollution of the soils with various organic pollutants. Neither detailed monitoring nor remediation measures were provided in the area of the city. The previous study [33] also confirmed elevated contents of both risk elements and PAHs in soils in the whole territory of the city, including recreational zones, city parks, and gardening colonies. Therefore, a detailed screening of the fungal species growing naturally in these areas, including the PAH contents in the fruiting bodies, was provided within the city area to identify the species among them that have promising potential for biodegradation of these compounds. The main objective of this study was to select indigenous fungal species to be used in further bioremediation measures to achieve satisfactory remediation efficiency in these long-term polluted areas.