## Results and Discussion
### Vibration Spectroscopy  
#### 3.2.1 FT MIR  
The average normalized FT MIR spectra of purified basidiocarps are represented in Figure 1a. The intense highly overlapped bands at 1200-950 cm\({}^{-1}\), which were assigned mainly to C-C and C-O stretching vibrations in pyranoid rings, are characteristic for polysaccharides [34]. Among these bands, the band near 1150 cm\({}^{-1}\) was found for all samples and assigned to the C-O-C stretching vibration of glycosidic bonds. The envelope shape of this region is characteristic for the mixture of fungal cell wall polysaccharides, mainly branched (1-3)(1-6)-\(\beta\)-p-glucan, linear (1-3)-\(\alpha\)-p-glucan and chitin; the IR bands or shoulders near 1375, 1316, 1253, 1202, 1150, 1071-1076 and 1033-1040 cm\({}^{-1}\) found for all samples are characteristic for fungal \(\beta\)-p-glucans [5; 14; 26]. In addition, the broad and weak IR band near 897 cm\({}^{-1}\) arose from C1H bending in \(\beta\)-anomeric sugar units of chitin and \(\beta\)-p-glucan [14; 35; 36]. By contrast, the weak bands at 930 and 847-853 cm\({}^{-1}\) indicated the presence of \(\alpha\)-p-glucans [36; 37; 38]. [PERSON] et al. [39] identified individual glucans in basidiocarps of _Boletus_ mushrooms by characteristic IR bands at 1155, 1025, 930 and 850 cm\({}^{-1}\) (\(\alpha\)-glucans) and at 1160, 1078, 1041 and 890 cm\({}^{-1}\) (\(\beta\)-glucans). Two IR bands at 1632-1650 cm\({}^{-1}\) (amide I) and 1554-1535 cm\({}^{-1}\) (amide II) were assigned to amide vibrations of chitin and proteins [40; 41]. The former band is overlapped by that of water in-plane deformation near 1640 cm\({}^{-1}\). For high-protein samples (po4, 3029), the amide II band was found near 1535 cm\({}^{-1}\), while in the case of some samples containing less protein (kryos, hk35 and p80), there was another band near 1550-1554 cm\({}^{-1}\) attributed to \(\alpha\)-chitin. Intensities of these amide bands highly varied for the samples and increased with the protein content. Similarly, the intensity of another band near 1236 cm\({}^{-1}\) was more pronounced for samples rich in protein (po4, 3009 and 3029), but was only \begin{table}
\begin{tabular}{c c c c} \hline \hline \multirow{2}{*}{**Sample**} & \multicolumn{3}{c}{**Glucans (\% _w/w_) 1**} \\ \cline{2-3}  & **Total** & \(\alpha\)**-Glucans** & \(\beta\)**-Glucans** \\ \hline
2013 & \(48.44\pm 2.01\) c & \(8.98\pm 0.27\) cd & \(39.46\pm 1.61\) bcde \\
2515 & \(45.99\pm 1.13\) c & \(13.85\pm 0.14\) e & \(32.14\pm 0.82\) bc \\
3009 & \(55.31\pm 1.56\) c & \(8.66\pm 0.17\) c & \(46.65\pm 1.35\) def \\
3029 & \(43.54\pm 2.80\) bc & \(4.89\pm 0.52\) ab & \(38.79\pm 1.97\) bcde \\
3253 & \(52.51\pm 1.44\) c & \(11.42\pm 0.30\) dc & \(41.10\pm 3.06\) cdef \\ fotios & \(45.45\pm 2.30\) bc & \(5.45\pm 0.43\) b & \(40.03\pm 1.96\) bcde \\ hk35 & \(49.22\pm 2.55\) c & \(5.22\pm 0.61\) ab & \(44.00\pm 1.75\) cdef \\ kryos & \(18.79\pm 3.39\) a & \(2.94\pm 0.46\) ab & \(15.85\pm 2.51\) a \\ spoppo & \(50.58\pm 2.85\) c & \(11.76\pm 0.93\) de & \(38.86\pm 2.29\) bcde \\ po1 & \(30.39\pm 2.95\) ab & \(3.15\pm 0.25\) ab & \(27.23\pm 2.25\) ab \\ po2 & \(56.17\pm 2.79\) c & \(4.63\pm 0.81\) ab & \(51.54\pm 1.80\) ef \\ po3 & \(48.32\pm 3.22\) c & \(9.03\pm 0.14\) cd & \(39.29\pm 2.77\) bcde \\ po4 & \(45.98\pm 2.08\) c & \(12.43\pm 0.30\) e & \(33.56\pm 1.57\) bcd \\ po5 & \(58.17\pm 3.14\) c & \(9.55\pm 0.68\) cd & \(48.62\pm 1.80\) ef \\ po6 & \(50.84\pm 3.39\) c & \(16.72\pm 0.48\) f & \(34.12\pm 2.37\) bcd \\ p80 & \(57.89\pm 2.60\) c & \(2.52\pm 0.19\) a & \(55.36\pm 2.33\) f \\ \hline \hline \end{tabular}  
* The statistical significance between mean values was calculated using ANOVA with Tukey HSD test (\(p<0.05\)); a–f are in sequence in accordance with statistically significant differences between samples.  
\end{table} 
Table 4: Composition of basidiocarps: total, \(\alpha\)- and \(\beta\)-glucans (mean \(\pm\) SD). a shoulder of the band at 1253 cm\({}^{-1}\) for the low-protein samples (kryos, hk35 and p80).