## 3 Results and Discussion
### Pseudomonas jordanii Strain G34 Genome Insight
Additionally, genes involved in IAA biosynthesis, a pivotal plant  
Figure 4: Pangenome analysis results: (**A**) Comparing the genomes of _P. jordanii_ strain G34 with _P. argentinensis_ LMG22563, with _P. stutzeri_ as an outgroup. (**B**) Comparing the genome of _P. jordanii_ strain G34 with 21 closely related species. The red lines denote singletons, representing genes that lack sequence homology with genes in any other genomes; Non-core genes are represented by the cyan lines; Clade-specific core orthologs identified in all genomes except the outgroup are represented by the purple lines; The dark blue lines represent core orthologo sets with at least one gene from the orthologo set present in each of the genomes; Pangenome non-core orthologs between core and singleton clusters present in more than one genome are represented by the grey lines. -hormone, were observed, indicating their potential significance in the symbiotic interactions between plants and bacteria [51]. Genes potentially involved in various crucial processes such as plant cell wall modification, secretion, membrane proteins, and transport systems, along with transcriptional regulators, ammonia and sulfur assimilation, and siderophores production, were identified. Furthermore, genes associated with tolerance mechanisms against osmotic and heat stress, detoxification, and heavy metal stress, including cobalt, zinc, and copper, were also detected (Table S4). Moreover, the presence of antibiotic resistance genes against fluoroquinolones, bleomycin, and fosmidomycin was detected (the annotated genes list is available online: BioProject PRJNA991609). Notably, the _ACC deaminase_ gene, often linked to plant growth  
Figure 5: (**A**) The graphical circular display illustrates the distribution of genome annotations for _P. jordanii_ strain G34. The outer to inner rings describe contigs (1), coding sequences (CDS) on the forward (2) and reverse (3) strands, GC content (4), and GC skew (5), RNA genes (6), CDS with homology to known virulence factors (7), and CDS with homology to known antimicrobial resistance genes (8). (**B**) The functional classification of predicted genes in the _P. jordanii_ G34 genome performed using RASTtk annotation. promotion in some endophytes, was absent in _P. jordanii_ strain G34, suggesting alternative mechanisms for its contribution to alleviate stress effects on plant growth [52]. Several metabolic pathways identified in _P. jordanii_ strain G34 hint at its potential for promoting plant growth and align well with its endophytic nature. These pathways include plastoquinone biosynthesis, carbon fixation, carotenoid biosynthesis, nitrogen metabolism, sulfur metabolism, and steroid hormone biosynthesis. Moreover, enzymes associated with the degradation of phenol, starch, sucrose, and xenobiotics were also detected, suggesting potential applications in the bioremediation industry. Notably, the presence of antibiotic biosynthesis monooxygenase hints at a possible role in the biosynthesis of aromatic polyketides. Given the draft genome of _P. jordanii_ strain G34 as a new species, future investigations into its transcriptome and molecular analyses are needed to offer insights into the functions of the novel proteins identified herein. These studies hold promise for uncovering the mechanisms through which this endophyte enhances plant growth [53]. Recent research on the closely related species _P. argentinensis_ LMG22563 revealed its effectiveness in enhancing plant growth under drought conditions through ABA-mediated root architecture and epigenetic reprogramming [54]. Additionally, _P. jordanii_ strain G34 demonstrated efficacy in enhancing growth under both greenhouse and field conditions and in mitigating negative salinity effects in a salt-sensitive genotype [43]. However, further investigation is needed to elucidate genotype-specific responses and the molecular mechanisms underlying genotype-bacteria interactions under saline conditions.