## 2 Results and Discussion  
Compound **1** was obtained as a white amorphous powder. Its molecular formula was established as C\({}_{28}\)H\({}_{38}\)O\({}_{6}\) by the positive HRESIMS, indicating six degrees of unsaturation. The \({}^{1}\)H-NMR spectrum of **1** (Table 1), showed several characteristic signals of the common withanolide steroid. Three signals at \(\delta\) 0.78 (3H, _s_), 1.18 (3H, \(d\), \(J\) = 6.8 Hz), 1.84(3H, _s_) and 2.10 (3H, _s_) were attributed to Me-18, Me-21, Me-19 and Me-28, respectively. A Me-27 signal was missing, and was replaced by two doublets at \(\delta\) 4.37 (1H, \(d\), \(J\) = 11.7 Hz) and 4.30 (1H, \(d\), \(J\) = 11.7 Hz), suggesting that C-27 was substituted by hydroxyl group. The double doublet at \(\delta\) 3.47 (1H, _dd_, \(J\) = 10.9, 4.5 Hz) was characteristic for a 12\(\beta\)-hydroxywithanolide [4]. H-22 resonated as a double triplet at \(\delta\) 4.62 (1H, _dt_, \(J\) = 12.9, 3.4 Hz), revealing a \(R\) configuration at C-22 [5, 6]. The \({}^{13}\)C-NMR spectrum of **1** showed resonances for all 28 carbons. The characteristic downfield signal at \(\delta\) 175.5 was due to two lactone carbonyls, respectively, along with the characteristic doublets at \(\delta\) 126.3, 157.9 and 168.6 were attributed to C-24, C-25 and C-26 of the \(a\),\(\beta\)-unsaturation-\(\gamma\)-lactone ring respectively, in the ring E. The signals at \(\delta\) 118.1, 130.2, 126.5 and 142.5 for the vinylic carbons at C-3, C-4, C-5, C-10 respectively, in the ring A. The typical signals at \(\delta\) 74.8, 78.6, 80.8, and 56.3 were assigned to the oxygenated carbons at C-6, C-12, C-22 and C-27, respectively. The signals appearing at \(\delta\) 8.1, 15.6, 15.3 and 20.2 were assigned to the Me-18, Me-19, Me-21 and Me-28, respectively. In addition, a signal at \(\delta_{\rm H}\) 5.30 (1H, _br. s_) together with the signal in the \({}^{13}\)C NMR spectrum of a keto-carbonyl of C-1 upfield of \(\delta_{\rm C}\) 175.5 due to an ester group of CO-O-CH indicated that **1** possesses a 1,10-_seco_-steroid skeleton [10, 11]. Further support this assumption was obtained from the fact that the methylene protons at C-2 [\(\delta\) 4.03 (1H, _br. d_, _J_=17.4 Hz) and 3.08 (1H, _dd_, _J_=17.4, 8.7 Hz)] were  
Figure 1: Structures of **1** and **2**. unusually shifted to a low field, suggesting the methylene to be situated between an ester carbonyl group and carbon-carbon double bond. Based on this finding and HMBC correlations, between C-1 (\(\delta_{\rm C}\) 175.5) and H-2\(a\), H-2\(\beta\), and H-3, between H-4 (6.62 (1H, \(dd,J=11.4\), 3.1 Hz) and C-2, C-5, C-6, and C-10, and between C-5 and Me-19, as shown in Figure 2. Thus, a seven-membered \(\beta\), \(\gamma\)-unsaturated lactonic moiety was determined in ring A. The \(\beta\)-configuration of the lactone bond at C-6 was established by a NOESY experiment (Figure 2). The NOESY spectrum showed the correlation between H-6 (1H, _br. s_) and H-2\(a\), H-7\(a\) and H-7\(\beta\), indicating that H-6 has the same configuration as H-2\(a\). Since a small coupling between H-2\(a\) and H-3 in the \({}^{1}\)H NMR spectrum was observed due to an approximate 90\({}^{\circ}\), a \(\beta\)-configuration of the lactone bond at C-6 was inferred, which is in agreement with those of 1,10-seco steroids [10,11]. Therefore, the structure of **1** was deduced as (12\(\beta\),6\(\beta\),22\(R\))-1,10-seco-6,12,27-trihydroxy-26-oxo-witha-3,5,24-trienolide-1-oic acid-\(e\)-lactone, which was named baimantuoluoline G.  
Compound **2** was obtained as a white amorphous powder and showed positive results for the _Molish_ reagent, which was indicative of a withanolide glycoside. Its molecular formula was established as C\({}_{34}\)H\({}_{50}\)O\({}_{12}\) by the positive HRESIMS, indicating 10 degrees of unsaturation. The \({}^{1}\)H-NMR spectrum of **2** showed distinct resemblance to those of baimantuoluoside G ((5\(\alpha\),6\(\beta\),22R)-5,6,27-trihydroxy-1-oxowitha-2,24-dienolide-27-O-\(\beta\)-glucopyranoside) [6].