## 5 Conclusions  
In conclusion, a total of 104,542 unigenes were identified from RNA-seq which were used as mapping reference of six samples of artificial PHS-induced and ABA-treated 'Keumgang' and 'Woori'. Among them, 123 unigenes were identified to be related to hormone metabolism and signaling pathways of phytohormones, ABA, GA, IAA, and cytokinin and 1862 of differentially expressed genes. The transcriptome analysis of phytohormone metabolism and signaling related genes and GO categorization of DEGs suggest that the main factor affecting PHS is ABA and ABI-mediated signaling (Figure 7). These results provided the data of transcriptional changes during PHS and consequently provide the clues for understanding processes of PHS and seed dormancy.  
**Supplementary Materials:** The following are available online at [[https://www.mdpi.com/article/10.3390/app11083562/s1](https://www.mdpi.com/article/10.3390/app11083562/s1)]([https://www.mdpi.com/article/10.3390/app11083562/s1](https://www.mdpi.com/article/10.3390/app11083562/s1)), Figure S1: Network analysis of DEGs group-V to VIII, Table S1: Categorization of group-I differentially expressed genes (DEGs), Table S2: Categorization of group-II differentially expressed genes (DEGs), Table S3: Categorization of group-III differentially expressed genes (DEGs), Table S4: Categorization of group-IV differentially expressed genes (DEGs), Table S5: Categorization of group-V differentially expressed genes (DEGs), Table S6: Categorization of group-VI differentially expressed genes (DEGs), Table S7: Categorization of group-VII differentially expressed genes (DEGs), Table S8: Categorization of group-VIII differentially expressed genes (DEGs), Table S9: Expression of unigenes related in ABA hormone metabolism and signaling, Table S10: Expression of unigenes related in IAA hormone metabolism and signaling, Table S11: Expression related in cytokinin hormone metabolism and signaling, Table S12: Expression of unigenes related in cytokinin hormone metabolism and signaling, Table S13: List of selected ABA, GA, IAA, Cytokinin pathway genes and gene identification. Constructed pathway gene specific primers are listed as forward (F) and reverse (R) primers used in qRT-PCR analysis.  
Figure 7: Proposed schematic diagram of the DEGs identified by RNA-seq transcriptome analysis in this study.  
**Author Contributions:** Data curation, Y.J.L.; Writing-Original draft preparation, Y.J.L.; Investigation, S.Y.P.; Validation, S.Y.P.; Writing-Reviewing, S.Y.P.; Editing, S.Y.P., D.Y.K. and J.Y.K.; Conceptualization, J.Y.K.; methodology, D.Y.K., J.Y.K.; Funding acquisition, J.Y.K.; Supervision, J.Y.K. All authors have read and agreed to the published version of the manuscript.  
This research was funded by Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ0148232021)\" Rural Development Administration, Republic of Korea.  
Not applicable.  
Informed Consent Statement: Informed consent was obtained from all subjects involved in the study.  
Raw sequencing data have been deposited in the NCBI Bioproject ([[https://www.ncbi.nlm.nih.gov/bioproject/PRJNA658641](https://www.ncbi.nlm.nih.gov/bioproject/PRJNA658641)]([https://www.ncbi.nlm.nih.gov/bioproject/PRJNA658641](https://www.ncbi.nlm.nih.gov/bioproject/PRJNA658641)), accessed on 22 August 2020) under the 6 accession numbers (SRX8986259, SRX8986260, SRX8986261, SRX8986262, SRX8986263, SRX8986264) in Sequence Read Archive (SRA).  
This research was supported by the National University Development Project by the Ministry of Education in 2020.  
The authors declare no conflict of interest.