## 1 Introduction  
Allergy, which is a chronic inflammatory disease, is a type of hypersensitivity reaction caused by an abnormally occurring immunological mechanism [1]. It is interpreted as an immune response that causes stimulatory or harmful actions from a wide range of meanings such as altered states and adverse reactions due to repeated exposure to an external stimulus, that is, an antigen [2]. Causes of allergies include dust, pollen, grass, pet dander, mold, and so on. In addition, allergies are usually immunoglobulin E (IgE)-mediated hypersensitivity reactions that can cause abnormal hypersensitivity reactions to inflammation [3]. The prevalence rate of allergic diseases has been on the rise in recent years because of the development of industrialization. Notably, the incidence rate of allergic rhinitis (AR) has increased rapidly [4]. AR is a chronic inflammatory disease that occurs in the nasal cavity mediated by IgE. IgE antibodies can bind to antigens to form a complex that can bind to the membrane surface of basophils and mast cells. After that, these cells produce histamine, prostaglandin, and leukotrienes. These chemical mediators cause various pathological outcomes such as smooth muscle contraction, vascular permeability, vasodilation and flushing, mucous secretion, and pruritus. Consequently, clinical symptoms of AR such as clear runny nose, paroxysmal sneezing, itching, rhinorrhea, and nasal congestion [5; 6; 7]. They begin at 1 to 2 min after antigen inflow. Symptoms usually disappear within one hour. If AR persists for a long time, various chronic inflammatory diseases such as sinusitis, asthma, nasal polyposis, and serous otitis media occur [8; 9]. Current treatments for AR can only alleviate symptoms not eliminate the cause of disease, because it is almost caused by environmental factors. It is classified as seasonal and perennial factors. Seasonal factors of allergen originate from pollen of grasses, trees, and weeds. And, perennial factors of allergen are originated from dust mites, fungi, animal dandruff, and other occupational factors [10; 11]. The symptomatic treatment uses medications such as corticosteroids, antihistamines, cromolyn, anti-cholines, and leukotriene receptor antagonists depending on symptoms. However, side effects are also occurring when drugs are used for a long time. Immunotherapy is considered if they do not respond to these medications [12]. It is effective in improving symptoms and quality of life. However, it has the disadvantages of requiring long-term treatment of 3 years or more and a high cost [13]. Experimental AR model in mice can be established by administering the unsensitized proteins to the nasal cavity and respiratory tract in mice because AR is not spontaneously caused in mice [14]. OVA, house dust mite, and fungus are the widely used allergens to induce the experimental AR model in mice. Among them, OVA is the universally used reagent for experimental allergen because of its low cost, high purity, and advantage to the study mechanism by using the genetic manipulation mice for OVA [15]. It is the major constituent protein in egg white, which represents 60-65% of the total protein [16]. So, it can work as an allergen to the mice, which are bred on the OVA-free feed. LPS-eliminated pure OVA is ordinarily co-sensitized with an adjuvant such as aluminum hydroxide (alum), heat-killed _Bordetella pertussis_, and complete Freund's adjuvant (CFA) in order to enhance the inflammatory reaction. Alum and _Bordetella pertussis_ boost Th2 responses, and CFA boosts Th1 responses. [17; 18; 19]. A two-step procedure to administrate allergen is commonly accepted and used: sensitization phase (general sensitization) and challenge phase (local sensitization). The sensitization phase is generally accomplished by administrating allergen through intranasal (IN), intraperitoneal (IP), or subcutaneous (SC) [20]. IP injection of antigen is universally adopted in asthma and AR models because it can produce IgE more than IN [21].