## 3 Experimental Results
The wettability measurements showed a significant decrease in the contact angle (Figure 10). (Supplementary Materials Figure S4). The changes are coherent with the roughness parameters; however, the dynamics of the process differed for both test methods. Such a phenomenon indicates a possible worsening of the surface's cleaning, which has a major impact on safety in hospitals. In addition, one must also take into account the influence of the degradation process on the bacteria's nesting ability on the surface. This issue may play a certain role in the decrease of the bacteriostatic efficacy of the material. It should be underlined that as AFM measurements may provide the majority of essential data for determining the product's aging ratio, this particular diagnostic technology reaches its maturity while appropriate measurement protocols and data processing data are developed and tested [31; 32; 33; 34; 35]. The usefulness of this approach and its complementary value to other methods used in this work is continuously tested and proven. The colorimetric analysis (according to CIELAB color space) [36] revealed a decrease in the luminance (\(L\) value) along with a change in the color component parameters (Figure 11): \(a-\) (red/green axis value) the decrease shows a shift to green color, and \(b-\) (yellow/ blue axis value) the increase shows a shift to yellow color. The arrows shown in Figure 11 (Supplementary Materials Figure S5) present the shift of certain components values, while the vertical bars show the standard deviation of values obtained from whole digital image of investigated sample. The obtained data show the major cause of the material's degradation, which is photo-oxidation. One could take into account the aesthetic issue, as the film is  
Figure 10: The wettability changes of the film surface for tested samples. Figure 9: The comparison of maximum pull force for investigated samples. planned to be used in equipment for everyday use. It has to be emphasized that below a certain thickness, the film can not be investigated using the colorimetric analysis, as the distances between the material-photon interactions are insufficient. Again, coherently to the AFM measurements, the analysis showed that the changes in the film properties after aging with dose B can still be acceptable (the color change was close to distinction), while for dose C, the discoloration is significant. The antibacterial activity of Ag NP films was investigated in a specialized biological laboratory, according to the ISO 22196:2007 standard [14]. The bacteriological test using _Escherichia coli_ ATCC25922 was performed. Two samples were delivered: the aluminum profiles covered with acrylic-based film containing the silver nanoparticles and acrylic-based film without Ag NPs (control sample). On both surfaces, 0.4 mL of fluid containing microorganisms was placed. Then, samples were stored in a humid environment. In order to determine the amount of bacteria, the inoculum was washed from the tested surfaces and incubated using TSA agar. The incubation lasted 24 h at 37 \({}^{\circ}\)C. The amount of living _Escherichia coli_ bacteria in the inoculum was 1.2 \(\times\) 10\({}^{6}\) jtk/mL. The test results are shown in Table 3.  
The results revealed satisfying efficacy of the investigated film in terms of bacteriostatic properties, while the R parameter was 5.30 log (78.3%).