###### Abstract
Free NH\({}_{3}\) can easily diffuse through cell membrane and accumulate in cytoplasm of algal cells, being toxic for most strains of microalgae due to its uncoupling effects on photosynthesis in chloroplasts [16; 17]. Thus, ADPE pretreatment are required to alleviate the color inhibition and ammonia toxicity for subsequent microalgae cultivation. [PERSON] et al. [18] reported that ammonia stripping as an energy-intensive pretreatment could only maintain the level of NH\({}_{4}\)+ from ADPE below the microalgae tolerance, but the dark color still inhibited algal growth. Physical and chemical methods such as electro-fenton coagulation and inorganic coagulants are already known for ADE decolorization [19; 20], but these methods are energy intensive and high cost, which do not comply with economic viability. Dilution method may be an economic solution overcoming the dark color of ADPE by dilution fold. However, in most previous studies, ADPE and other anaerobically digested manure needed high dilution to reduce color inhibition and NH\({}_{4}\)+ toxicity before microalgae cultivation [11; 21]. [PERSON] et al. [11] investigated that the algal yield of _Chlorella vulgaris_ peaked at an initial concentration of ammonium nitrogen (NH\({}_{4}\)+\(-\)N) of 100 mg L\({}^{-1}\) in 50-fold diluted ADPE but was inhibited at higher NH\({}_{4}\)+\(-\)N concentrations in 10- and 20-fold diluted ones. [PERSON] et al. [21] showed that higher algal yields were obtained at initial NH\({}_{4}\)+-N concentration of 110 mg L\({}^{-1}\) in 20-fold diluted ADE from dairy manure rather than 10-, 15-, and 25-fold diluted ones during _Chlorella_ sp. culture. These results might be because NH\({}^{+}\)-N at a concentration of 110 mg L\({}^{-1}\) was critical for NH\({}_{4}\)+ tolerance in green algae, including _Chlorella_ spp. [22]. Indeed, high dilution pretreatment can alleviate the color inhibition and ammonia toxicity in ADPE but increase water consumption and reduce the nutrient concentrations, limiting algal growth. To reduce the high dilution of ADPE, a decolorization pretreatment of ADPE using NH\({}_{4}\)+-tolerant algal species may be required. In recent years, fungi have become promising microorganisms in wastewater decolorization due to their low energy consumption and cost [15; 23; 24], compared to electrocoagulation and chemical reagents. Fungi have a strong ability to degrade complex organic compounds during decolorization by producing extracellular enzymes, such as lacase, manganese peroxidase and lignin peroxidase [25]. For example, [PERSON] and [PERSON] [24] isolated a fungal strain, _Achromobacter xylos oxidans_ GRIRKNM11, from a textile dye effluent site, which could decolorize turquoise blue dye within 48 h. Currently, fungal decolorization is mostly used in the treatments of the paper mill and textile dyes' effluent [25]. For microalgae cultivation in wastewater through fungal decolorization, only [PERSON] al. reported that _Planerochaete chrysyosporium_ could achieve decolorization of 80% from ADPE at a pH of 6.3 and 25 \({}^{\circ}\)C [15], and _Chlorella vulgaris_ could survive in the decolorized ADPE but with low removal rates of <12% for NH4\({}^{+}\)-N, TP, and COD [26]. The authors only focused on the feasibility of algal survival in the decolorized ADPE and its potential value of biofuel, but did not enhance the algal yield and nutrient removal from the ADPE through process optimization of microalgae cultivation [26]. Therefore, the integrated process-optimization strategy of fungal pretreatment and subsequent microalgae cultivation may be a more reliable procedure for algal biomass production and nutrient removal from ADPE. This novel concept of fungal pretreatment with high decolorization provides a promising way for microalgae-based wastewater remediation. The concept is needed to further optimize process to enhance algal yield and nutrient removal in microalgae-based wastewater remediation, including ADPE. This study was designed to solve the technical problems encountered in ADPE remediation using microalgae.