## 3 Results and Discussions
### Liver Biomarkers and Hepatocellular Injury Indicators  
The data in Table 5 represent the concentration of plasma ALT, AST, and ALP of the various investigated groups. A significant alteration was noticed in the liver biomarkers in rats subjected to drinking tap water (positive control (+)) and distilled water mixed with HMs (G3-1) compared with distilled water only (negative control (-)). According to the literature [61], toxic metals have negative effects on hepatic functions, biosynthetic efficiency, and the integrity of hepatocytes. The liver weight was significantly lower in control+ and G3-1 than in Control-; however, it was significantly increased in all treatments other than control+ and G3-1. In addition, all of the examined groups (G3-2, G3-3, G3-4, and G3-5) showed a significant reduction in the mean values of ALT than control+ and G3-1. G3-3 and G3-5 exhibited the least ALT values compared with the other treatments, even though G3-5 was less than for Control-, with an insignificant difference. Control+ and G3-1 caused an elevation in AST levels than Control-; however, all other groups recorded a significant decrease compared with control+ and G3-1. The lowest decline in AST was also noticed in G3-5. The tap water and mixed HMs also caused an incremental change in ALP levels; however, G3-2, G3-3, and G3-5 showed a significantly more decreased ALP level than for Control-, control+, and G3-1. It is noticed also that G3-3 and G3-5 recorded the best results for ALT and AST compared with the other treatments. It is shown in [62] that an increase in ALT and AST levels that is out of proportion to ALP and bilirubin implies hepatocellular disease. An increase in ALP and bilirubin levels disproportion to ALT and AST would mean a cholestatic pattern. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**RT** & **Compound Name** & **Area \%** & **\% Peak Area** & **Formula** & **MW** \\ \hline \multirow{4}{*}{29.20} & \multicolumn{3}{c}{Cyclopropanebutanoic acid, 2-[[2--[2-[(2- & & \\  & pentylcyclopropyl)methyl]cyclopropyl]} & 2.52 & 42,105,270 & C\({}_{25}\)H\({}_{42}\)O\({}_{2}\) & 374 \\  & methyl], methyl ester & & & & \\
30.92 & 9-Octaldecenoic acid & 2.00 & 33,361,130 & C\({}_{18}\)H\({}_{34}\)O\({}_{2}\) & 282 \\
32.40 & 7-Methyl-Z-tetradecen-1-ol acetate & 6.34 & 105,891,316 & C\({}_{17}\)H\({}_{32}\)O\({}_{2}\) & 268 \\
34.20 & 9,12-Octadecadienoic acid & 2.24 & 37,389,728 & C\({}_{18}\)H\({}_{32}\)O\({}_{2}\) & 280 \\
44.13 & Ethly iso-allocholate & 3.31 & 55,267,353 & C\({}_{26}\)H\({}_{44}\)O\({}_{5}\) & 436 \\  & \multicolumn{3}{c}{**Barley**} \\ \multirow{2}{*}{8.92} & Dodecane & 17.03 & 126,128,138 & C\({}_{12}\)H\({}_{26}\) & 170 \\
32.54 & 9 Octadecenoic acid & 42.19 & 312,497,160 & C\({}_{18}\)H\({}_{34}\)O\({}_{2}\) & 282 \\
44.49 & Ethyliso-allocholate & 40.78 & 302,104,307 & C\({}_{26}\)H\({}_{44}\)O\({}_{5}\) & 346 \\ \hline \hline \end{tabular}
\end{table} 
Table 4: _Cont._  
Figure 2: Acetylcholinesteraseterase (AChE) level in rat brains. Values with different letters in each column showed a significant difference. According to [63; 64], acute HM into intoxations may damage the liver, along with many other organs in the body. [PERSON] et al. [24] stated that wheatgrass juice contains many enzymes that assist in cleansing the body of contaminants and toxins; in addition, amino acids facilitate in detoxifying the liver and removing harmful HMs from the bloodstream, eliminating toxins from the body, and slowing down the ageing process. [PERSON] et al. [65] found that the leaf extract of wheatgrass influences the activities of liver enzymes and lipid peroxidation. It has been proven that certain biologically active phytochemicals are released during germination. [PERSON] et al. [66] stated that wheatgrass treatment suppressed the increase in liver enzymes based on its dose. [PERSON] et al. [27] reported that wheatgrass extract contains flavonoids (apigenin) that are known to enhance hepatic functions.