## 3 Outlook
It can be found that biopesticides are gaining popularity in developing countries represented by China. Biopsiesticks with higher safety, more environmental friendliness, and other advantages, in line with the developmental trends of modern agriculture, are gradually becoming mainstream in modern pesticide development. However, at present, natural biological pesticide types are still relatively unique and expensive with slow efficacy globally, and the development of new varieties and technologies is not yet comprehensive and rapid. The production of biopesticides has long suffered from bottlenecks, problems such as backward manufacturing processes, long production cycles, and an over-dependence on natural resources. Developing new compounds with novel structures and achieving the efficient production of known structures require the help of synthetic biology. Therefore, it is imperative to promote the application of synthetic biology in the field of biopesticide development for the development of the biopesticide industry. There are three main methods for the production of biopesticides: direct extraction from organisms, chemical synthesis, and biosynthesis. Common extraction methods include extraction and separation using organic solvents such as ethanol, extraction by supercritical fluid extraction (SFE), and ultrasonic cycle technology, which means adding ultrasonic cycle processing to organic solvent extraction. These methods can reduce the number of organic solvents used, shorten the working time, and improve the extraction rate. With the development of science and technology, separation techniques such as chromatography have also provided new ideas for the extraction of natural products [102]. However, the direct extraction method relies entirely on the plant itself, which grows slowly, has a long production cycle for natural products, and has a limited yield, making it difficult to carry out large-scale production. There are two main disadvantages of chemical synthesis. Firstly, many natural products are structurally complex and contain many chiral centers, thus, deactivated and difficult to separate spin isomers can occur during the process of performing synthesis. Secondly, the synthesis has more steps, low conversion rate, high energy consumption, and the organic reagents used can put serious pressure on the environment, making it difficult to meet the needs of industrialization [103]. The introduction of synthetic biotechnology into the development of botanical biopesticides can effectively solve the problems of limited resources, complex production processes, and low production efficiency faced by traditional production, thus reducing the production costs of biopesticides and lowering the price barriers to their application. These measures would make biopesticides truly affordable for farmers in China and effectively reduce the number of chemical pesticides applied for the benefit of the environment. Synthetic biology has a wide scope for development. In 2006, the biosynthesis of _artemisinic acid_ in _Saccharomyces cerevisiae_ was first reported in _Nature_[35]. In 2013, by introducing plant dehydrogenase and cytochrome CYB5, regulating gene expression, and using a two-phase fermentation method, the yield of _artemisinic acid_ was finally increased to 25 g/L\({}^{-1}\)[104], laying the foundation for its industrial production and also signals a promising future for synthetic biology. Breakthroughs have been made in the biosynthesis and synthetic biology of plant-derived biopesticides, but there are still many pressing issues that need to be addressed. For example, the biosynthetic pathways are not fully resolved, and the coordination and balance between components need to be overcome after multiple components are assembled together. The future of synthetic biology remains focused on three levels: chassis, pathways, and components. Firstly, select and modify a highly adaptable chassis.