## 2 Results and Discussion
### Analysis and Quantification of Atropine and Scopolamine in Different Types of Infusions
\begin{table}
\begin{tabular}{c c c c} \hline \hline
**Infusion Code** & **Sample Description (Code)** & \
\begin{tabular}{c} **Atropine** \\ **(ng/mL \(\pm\) SD)** \\ \end{tabular} &
\begin{tabular}{c} **Scopolamine** \\ **(ng/mL \(\pm\) SD)** \\ \end{tabular} \\ \hline \hline Tea-W-1 \({}^{\text{a}}\) & White tea (W-1) & 2.57 \(\pm\) 0.28 & 0.33 \(\pm\) 0.02 \\ \hline Tea-W-2 \({}^{\text{a}}\) & White tea (W-2) & 1.24 \(\pm\) 0.14 & 0.33 \(\pm\) 0.04 \\ \hline Tea-W-3 \({}^{\text{a}}\) & White tea (W-3) & 2.86 \(\pm\) 0.12 & ND \\ \hline Tea-G-1 \({}^{\text{a}}\) & Green tea (G-1) & 3.65 \(\pm\) 0.10 & 0.34 \(\pm\) 0.02 \\ \hline Tea-G-2 \({}^{\text{a}}\) & Green tea (G-2) & 4.65 \(\pm\) 0.57 & ND \\ \hline Tea-G-3 \({}^{\text{a}}\) & Green tea (G-3) & 4.94 \(\pm\) 0.74 & \(\leq\)MQL \\ \hline Tea-G-4 \({}^{\text{a}}\) & Green tea (G-4) & 4.15 \(\pm\) 0.24 & ND \\ \hline Tea-G-5 \({}^{\text{a}}\) & Kukicha green tea (G-5) & \(\leq\)MQL & ND \\ \hline Tea-B-1 \({}^{\text{a}}\) & Black tea (B-1) & 1.81 \(\pm\) 0.14 & ND \\ \hline Tea-B-2 \({}^{\text{a}}\) & Black tea (B-2) & 1.65 \(\pm\) 0.07 & ND \\ \hline Tea-B-3 \({}^{\text{a}}\) & Black tea (B-3) & 1.30 \(\pm\) 0.16 & ND \\ \hline Tea-B-4 \({}^{\text{a}}\) & Black tea with bergamot (B-4) & 1.95 \(\pm\) 0.08 & ND \\ \hline Tea-B-5 \({}^{\text{a}}\) & Black tea (B-5) & 2.00 \(\pm\) 0.16 & ND \\ \hline Tea-B-6 \({}^{\text{a}}\) & Black tea (B-6) & 0.44 \(\pm\) 0.05 & ND \\ \hline Tea-B-7 \({}^{\text{a}}\) & Pakistani black tea (B-7) & 1.74 \(\pm\) 0.08 & ND \\ \hline Tea-B-8 \({}^{\text{a}}\) & Black tea (B-8) & 0.75 \(\pm\) 0.03 & ND \\ \hline Her-Inf-1 \({}^{\text{b}}\) & Pink lapacho bark tea (Her-1) & ND & ND \\ \hline Her-Inf-2 \({}^{\text{b}}\) & Lemon grass tea (Her-2) & ND & ND \\ \hline Her-Inf-3 \({}^{\text{b}}\) & Rosemary (Her-3) & ND & ND \\ \hline Her-Inf-4 \({}^{\text{b}}\) & Valerian (Her-4) & \(\leq\)MQL & ND \\ \hline Her-Inf-5 \({}^{\text{b}}\) & Echin (Her-5) & ND & ND \\ \hline Her-Inf-6 \({}^{\text{b}}\) & Star anise (Her-6) & ND & ND \\ \hline Herb-Inf-7 \({}^{\text{b}}\) & Flavored yerba mate (Her-7) & 0.14 \(\pm\) 0.01 & ND \\ \hline Herb-Inf-8 \({}^{\text{b}}\) & Flavored yerba mate (Her-8) & \(\leq\)MQL & ND \\ \hline Herb-Inf-9 \({}^{\text{b}}\) & Mixed herbal tea (Her-9) & \(<\)MQL & ND \\ \hline Herb-Inf-10 \({}^{\text{b}}\) & Mixed herbal tea (Her-10) & ND & ND \\ \hline Herb-Inf-11 \({}^{\text{b}}\) & Mixed herbal tea (Her-11) & \(<\)MQL & ND \\ \hline Herb-Inf-12 \({}^{\text{b}}\) & Mixed herbal tea (Her-12) & ND & ND \\ \hline Herb-Inf-13 \({}^{\text{b}}\) & Mixed herbal tea (Her-13) & ND & ND \\ \hline Herb-Inf-14 \({}^{\text{b}}\) & Mixed herbal tea (Her-14) & ND & ND \\ \hline Herb-Inf-15 \({}^{\text{b}}\) & Mixed herbal tea (Her-15) & ND & ND \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Contents of atropine and scopolamine in the tea and herbal tea infusions analyzed. Differences in the types of tea appear to influence the content of TAs. Samples of white and green teas, which do not undergo the oxidation process, generally have the highest content of TAs, with levels ranging from 1.24 to 4.94 ng/mL for atropine and around 0.33 ng/mL for scopolamine. However, Tea-G-5 (kukicha green tea) showed the lowest atropine content (\(\leq\)MQL) compared to the other green teas, and scopolamine contamination was not found. This may be because it is a green tea sample that comes only from the petioles, stems, and twigs of _Camelia sinensis_, allowing for easier differentiation when separating the product from weeds (_Datura stranonium, Hoycsumus niger_, _Solanum nigrum_, etc.) unlike other white or green teas made from leaves. In general, a lower concentration of TAs was found in black teas than in green and white teas, with only atropine being found in black tea samples (between 0.44 and 2.00 ng/mL). Lower TA amounts were observed in Tea-B-5 (0.44 ng/mL) and Tea-B-8 (0.75 ng/mL) from conventional farming.