## 2 Results and Discussion
### Terpene Organic Compound Profiles of Extracts
Terpene profiles are unique to each cannabis variety [33] and change throughout a plant's growth cycle. Sesquiterpene concentrations are higher during the early growth stages, while monoterpene concentrations increase over time [26]. After harvesting and drying the plant material, the terpene and terpenoid content decreases due to the volatility of these compounds. The prolonged storage of raw material and temperatures above 60 \({}^{\circ}\)C used during solvent removal from the extract also negatively impact the concentration of these volatile substances. [PERSON] et al. reported that the monoterpene-to-sesquiterpene ratio in extracts obtained using solvents is lower than in essential oils [23]. Moreover, in the context of practical applications of cannabis extracts, it is crucial to understand and analyze the influence of the so-called \"entourage effect\", referring to the impact of other natural compounds, such as terpenoids, present in the extract on its biological activity [34]. Literature data indicate that plant-based formulations are more effective than individual compounds [35], particularly in the context of anticancer and anti-inflammatory activity [36]. Furthermore, terpenes and cannabis exhibit synergistic interactions, enhancing their anticancer effects through the activation of CB2 receptors [37]. Therefore, it is essential to optimize cannabis extraction methods to achieve the highest concentrations of both groups of compounds. \begin{table}
\begin{tabular}{l c c c} \hline \hline
**Compound** & \(\text{LRI}_{\text{exp}}\)1  & \(\text{LRI}_{\text{lit}}\)2  & \(\text{Content Range}\) & \multicolumn{1}{c}{\(\text{Min-Max [\%]}\)} \\  & & & \\ \hline \(\alpha\)-Ylangene & 1374 & 1371 & 0.01–2.04 \\ \(\alpha\)-Copaene & 1378 & 1375 & 0.01–0.38 \\ \(\beta\)-Longipinene & 1410 & 1407 & T \\ \(\alpha\)-_cis_-Bergamotene & 1419 & 1416 & 0.01–2.07 \\ E-Caryophyllene & 1425 & 1424 & 0.02–40.35 \\ \(\beta\)-_cis_-Farmesene & 1435 & 1440 & 0.01–0.23 \\ \(\alpha\)-_trans_-Bergamotene & 1440 & 1432 & 0.03–13.78 \\ Azulene & 1448 & 1444 & 0.01–0.24 \\ Allaozmandendrene & 1455 & 1458 & T \\ \(\alpha\)-Humulene & 1458 & 1454 & 4.21–33.50 \\ Sesquisabinene & 1461 & 1455 & 3.33–17.14 \\ \(9\)-_epi_-(_E_)-Caryophyllene & 1466 & 1464 & 0.01–2.54 \\ \(\gamma\)-Gurjunene & 1481 & 1476 & 0.01–0.55 \\ \(\alpha\)-Amorphene & 1484 & 1482 & 0.01–0.58 \\ \(\beta\)-Chamigrene & 1488 & 1479 & 0.01–1.16 \\ \(\beta\)-Selinene & 1490 & 1492 & 0.01–3.06 \\ Unknown sesquiterpene & 1494 & & 0.03–5.24 \\ \(\alpha\)-Selinene & 1499 & 1501 & 0.01–3.36 \\ \(\beta\)-Bisabolene & 1512 & 1508 & 0.01–5.87 \\ \(\beta\)-Curcunene & 1515 & 1511 & 0.01–1.06 \\ \(\gamma\)-Cadinene & 1518 & 1512 & 0.01–0.90 \\ \(7\)-_epi_-\(\alpha\)-Selinene & 1523 & 1518 & T \\ \(\beta\)-Guaiene & 1526 & 1523 & T \\ Unknown sesquiterpene & 1528 & & 0.03–3.32 \\ Selina-4(15),/(11)-diene & 1541 & 1540 & 0.02–2.98 \\ Unknown sesquiterpene & 1543 & & 0.01–1.29 \\ Selina-3,/(11)-diene & 1548 & 1546 & 0.01–2.58 \\ (_E_)-Neroliolol & 1567 & 1561 & 0.01–0.78 \\ Caryophyllene oxide & 1589 & 1587 & 0.02–5.51 \\ Humulene epoxide I & 1592 & 1605 & 0.01–0.60 \\ Humulene epoxide II & 1615 & 1613 & 0.01–1.78 \\ Unknown sesquiterpene & 1657 & & 0.01–0.49 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: _Cont._