###### Abstract
\begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline \multirow{2}{*}{**Vitamins**} & \multirow{2}{*}{**Mobile Phases**} & \multirow{2}{*}{**Solvent**} & \multirow{2}{*}{\begin{tabular}{c} **Stationary** \\ **Phase** \\ \end{tabular} } & \multirow{2}{*}{\begin{tabular}{c} **Develop-** \\ **Develop-** \\ **ment Steps** \\ \end{tabular} } & \multirow{2}{*}{**Detection**} & \multirow{2}{*}{\begin{tabular}{c} **LOD and** \\ **LOQ** \\ **(ng/band)** \\ \end{tabular} } & \multirow{2}{*}{**Type of** \\ \multirow{2}{*}{\(\text{D}_{3}\)} & \multirow{2}{*}{\begin{tabular}{c} Chlorophyll: \\ Diethyl ether \\ (90:10, \(v/v\)) \\ \end{tabular} } & \multirow{2}{*}{Methanol} & 60F254S & \multirow{2}{*}{\(1\)} & \multirow{2}{*}{\(1\)} & \multirow{2}{*}{\(17.54\) and \\  & & & (20 \(\times\) 10 cm) & & & & & \\ \hline \multirow{2}{*}{\(\text{D}_{3}\)} & Ethanol: Water & \multirow{2}{*}{\((70.30,\,v/v)\)} & \multirow{2}{*}{Methanol} & RP-60F254S & \multirow{2}{*}{\(1\)} & \multirow{2}{*}{\(1\)} & \multirow{2}{*}{\(1\)} & \multirow{2}{*}{\(\text{Densitometry}\)} & 8.47 and \\  & & & (20 \(\times\) 10 cm) & & & & & \\ \hline \multirow{4}{*}{A and E} & Benzene and a & \multirow{4}{*}{\begin{tabular}{c} Methanol \\ aqueous \\ micellar \\ solution of \\ dodecyl \\ sulfate (SDS) \\ \end{tabular} } & \multirow{4}{*}{\begin{tabular}{c} High- \\ performance \\ Sorbfil plates \\ \end{tabular} } & \multirow{4}{*}{\(2\)} & \multirow{4}{*}{\(2\)} & \multirow{4}{*}{\(\begin{tabular}{c} Optical den- \\ stometer \\ and densitometry \\ \end{tabular} } & \multirow{4}{*}{\(-\)} & \multirow{4}{*}{\(\text{Standards}\)} & \multirow{4}{*}{\(\text{[16]}\)} \\  & & & & & & & \\ \hline \multirow{4}{*}{A and E} & Chloroform: & Methanol & 60F254 & \multirow{4}{*}{\(1\)} & \multirow{4}{*}{\(1\)} & \multirow{4}{*}{\(0.16\) and \(1.2\); \\  & Cyclohexane & \
\begin{tabular}{c} and \\ (55:45, \(v/v\)) \\ \end{tabular} } & \multirow{4}{*}{\(n\)-heptane} & \multirow{4}{*}{\(1\)} & \multirow{4}{*}{\(\text{Densitometry}\)} & \multirow{4}{*}{\(0.89\) and \(5.71\)} & \multirow{4}{*}{\(\text{Human}\)} & \multirow{4}{*}{\(\text{[18]}\)} \\  & & & & & & & \\ \hline \multirow{4}{*}{\(\alpha\)-, \(\beta\)-, \(\gamma\)-, \(\gamma\)-, and \(\delta\)- \\  & Ethanol-water & \multirow{2}{*}{Chloroform} & RP-18- & \multirow{2}{*}{\(1\)} & \multirow{2}{*}{\(1\)} & \multirow{2}{*}{\(\text{Dipyridyl}\)} & \multirow{2}{*}{\(-\)} & \multirow{2}{*}{\(\text{Various}\)} & \multirow{2}{*}{\(\text{[17]}\)} \\  & \(\text{\(9.01,\,\,9.50,\,5\);}\) & & & & & & \\ \hline \multirow{4}{*}{\(\text{K}_{1}\)} & Methanol: & \multirow{4}{*}{Ethanol:
\begin{tabular}{c} \\ Isopropanol: \\ Water \\ (75:5:5:15, \\ \(v/v/v/v\)) \\ \end{tabular} } & \multirow{4}{*}{Ethanol} & \multirow{4}{*}{\(1\)} & \multirow{4}{*}{\(\text{Densitometry}\)} & \multirow{4}{*}{\(0.19\)-\(0.85\)} & \multirow{4}{*}{\(\text{Pharmaceutical}\)} \\  & & & & & & & \\ \hline \multirow{4}{*}{\(\text{K}_{1}\)} & Methanol: & \multirow{4}{*}{Ethanol} & Silica gel & \multirow{4}{*}{\(1\)} & \multirow{4}{*}{\(\text{Densitometry}\)} & \multirow{4}{*}{\(0.57\)–\(2.50\)} & \multirow{4}{*}{\(\text{Pharmaceutical}\)} \\  & Isopropanol: & & & & & \\ \cline{1-1}  & Water & \multirow{2}{*}{\((20\times 10\text{\,cm})\)} & & & & & \\ \cline{1-1}  & & & & & & \\ \cline{1-1}  & & & & & & & \\ \cline{1-1}  & & & & & & & \\ \cline{1-1}  & & & & & & & \\ \cline{1-1}  & & & & & & & \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Published studies using HPTLC for the analysis of fat-soluble vitamins. including D\({}_{2}\), D\({}_{3}\), E, and K\({}_{1}\) (Figure 1) in pharmaceutical preparations using a simple mobile phase and a single development step.