## 4 Discussion  
Triticale is a breed plant generated by crossing wheat and rye, it is the first successfully obtained grain from two different grain plants, and it is used in the diet of domestic and farm animals by different ways. It has a high content of crude protein as well as carbohydrates which are the key factors for the production of concentrated animal feed. It can replace oats, fodder, barley, and other cereals [24]. Preservation of forages with rich nutrients for prolonged periods is challengeable due to loss of nutrients by plant oxidation, entero-bacteria growths, proteolytic activity, undesired fermentation, deamination and decarboxylation of amino acids strongly affects the quality of forage/silage which increases energy loss and anti-nutritional compounds in the forages [3]. The major objectives of the silage and feed industry are to improve the nutritional content, aerobic stability, and absorbance efficiency of plant silages. In general, lactic acid bacteria (LAB) plays a major role in the preservation of forages at ensiled condition by rapid induction of fermentation of silages. However, in the feed industry, indigenous LAB population in the plant is not sufficient to meet the current industry requirements. Therefore, additional inoculums are required to make good quality silages with preserved nutrients, particularly LAB. In the current study, potent lactic acid bacteria were isolated and identified by biochemical and molecular tools indicated that these strains belonged to the _L. rhamns_ and _L. paracasei_. These bacteria were able to ferment various carbohydrates substrates which varied among the strains and it produces different kind extracellular enzymes which are very useful in food and leather industries. These enzymes productions differed between the KCC-51 and KCC-52, it consisted with a previous report [31]. Antibiotic sensitivity of LAB is an essential criterion for the selection of potent probiotic strains. The isolated KCC-51 and KCC-52 possess good antibiotic sensitivity against almost all of the tested antibiotics, but slightly varied among the antibiotics [32]. In addition, KCC-51 and KCC-52 had greater antibacterial activity against _E. faecalis_, _E. coli_, _P. aeruginosa_, and _S. aureus_, which could be very useful in the prevention of pathogenic/undesirable microbial growths. Most of the LAB showed significant antibacterial activity against _E. coli_, _S. enteritidis_, _S. aureus_, and _E. faecalis_[29; 33]. As a good probiotic, strains must have strong antimicrobial activity, survive in the harsh condition of GIT such as resistant in gastric, duodenal intestinal juices, hydrophobicity and aggregation properties [34; 35]. Here we noted significant survival rates of both strains in the simulated gastric, duodenal, and intestinal juices. Strains were reduced initially in the gastric juice due to its lower acidic condition similar to the stomach, further transferring of these strains into the duodenal and intestinal juices showed more survival rates compared to gastric juice; it suggested that these strains obtained strong resistance capacity from low pH of the gastric juice. Bile salt and higher pH affect the probiotic growths [36; 37]. Moreover, the probiotic features have been proven for strains belonging to _L. rhamns_, _L. paracasei_, and _L. casei_[38] in accordance with FAO/WHO [38]. KCC-51 and KCC-52 showed good antibacterial and potential probiotic properties; next, we studied the impact of these strains on nutrient profiles, fermentative acids, and microbial changes in the ensiled silages at day 180. It suggested that the addition of KCC-51 and KCC-52 to forage samples when ensiling