## 3 Results and Discussion
### Phytochemical Analysis
Caffeoyl phenylpropanol, known as acteoside or verbascoside, is extensively found in the plant kingdom, particularly in the _Aloysia_ species [13]. Based on our analysis, both ethanolic and aqueous extracts are rich in acteoside. The second-most abundant compounds in the ethanolic extract were isoatecoside, eupatorin, pectolinarigenin, isorhamtein, leucoscopetoside A, crissilio1, apigenin, narigenin, hispidulin, and kaempfer. In the aqueous extract, luteolin 7-O-diglucuronide was a major component, as were apigenin 7-O-diglucuronide, eupatorin, vicenin-2, eriocitrin, and geniposidic acid. The majority of the identified compounds from the plant extracts have been reported to have significant biological activities. For example, verbascoside is a potent antioxidant agent. Thiobarbituric acid-reactive substances (TBARS) and trolox equivalent antioxidant \begin{table}
\begin{tabular}{c c c c c} \hline \hline  & \multicolumn{2}{c}{_Aloysia citrodota_ H\({}_{2}\)O Extract—Negative} & & \\ \hline \hline
**Row No.** & **Component Name** & **Observed \(m/z\)** &
\begin{tabular}{c} **Mass Error** \\ **(mDa)** \\ \end{tabular} & **Response** \\ \hline
1 & Luteolin 7-O-diglucuronide & 639.1197 & 0.5 & 655,236 \\
2 & Geniposidic-Acid & 397.1095 & \(-\)1 & 380,211 \\
3 & Acteoside & 647.1952 & 0.6 & 236,018 \\
4 & Apigenin 7-O-diglucuronide & 623.1246 & 0.3 & 134,959 \\
5 & Dimethylmatairesinol & 409.1614 & \(-\)0.8 & 124,934 \\
6 & Pinoresinol-4-O-Beta- & 543.1842 & 0.5 & 41,603 \\
7 & Monoglycoside & 647.1966 & 2 & 37,067 \\
8 & 4-Hydroxybenzoic acid & 323.0729 & \(-\)0.8 & 34,696 \\
9 & Eupatorin & 345.0958 & \(-\)1.1 & 17,851 \\
10 & Leucospectoide A & 661.2112 & 0.9 & 6345 \\
11 & Coumaric acid-4-O-glucose & 349.0882 & \(-\)1.2 & 4471 \\
12 & Hernairn & 177.0538 & \(-\)0.8 & 3055 \\
13 & Crissilio1 & 331.0797 & \(-\)1.5 & 2977 \\
14 & Apigenin-7-O-glucuronide & 447.0926 & 0.4 & 2594 \\
15 & Vicenin-2 & 617.1463 & \(-\)1.4 & 2533 \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Identified compounds in _A. citrodota_ aqueous extract- (ESI positive mode). \begin{table}
\begin{tabular}{c c c c c} \hline \hline  & \multicolumn{2}{c}{_Aloysia citrodota_ H\({}_{2}\)O Extract—Negative} & & \\ \hline \hline
**Row No.** & **Component Name** & **Observed \(m/z\)** &
\begin{tabular}{c} **Mass Error** \\ **(mDa)** \\ \end{tabular} & **Response** \\ \hline
1 & Luteolin 7-O-diglucuronide & 637.104 & \(-\)0.6 & 1,157,953 \\
2 & Acteoside & 623.198 & \(-\)0.2 & 971,531 \\
3 & Geniposidic-Acid & 373.1143 & 0.3 & 399,595 \\
4 & Isoacetoside & 623.1985 & 0.4 & 269,646 \\
5 & Malic acid & 133.0143 & 0.1 & 223,063 \\
6 & Apigenin 7-O-diglucuronide & 621.1094 & \(-\)0.3 & 202,647 \\
7 & 4-Hydroxybenzoic acid & 299.0772 & 0 & 54,020 \\
8 & Eupatorin & 343.0819 & \(-\)0.5 & 33,223 \\
9 & Leucospectoide A & 637.2129 & \(-\)0.9 & 23,761 \\
10 & Vicenin-2 & 593.151 & \(-\)0.2 & 22,973 \\
11 & Crissilio1 & 329.0665 & \(-\)0.2 & 14,545 \\
12 & Pectolinarigenin & 313.0716 & \(-\)0.2 & 13,039 \\
13 & Protocatechuric acid & 315.0723 & 0.1 & 12,191 \\
14 & Ericoitrin & 595.1663 & \(-\)0.6 & 11,704 \\
15 & Coumaric acid (p-) & 163.0399 & \(-\)0.2 & 8432 \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Identified compounds in _A. citrodota_ aqueous extract- (ESI negative mode). capacity (TEAC) assays revealed a protective effect on the ocular tissue and fluids against oxidative stress following oral administration of verbascoside to hares (maximum dose of 3 mg/day) [11]. [PERSON] et al. [25] reported that verbascoside alleviated experimental colitis in mice by regulating the gut microbiota. The outcomes demonstrated that in colitic mice, acetoside reduces intestinal inflammation, oxidative stress, and nuclear factor-B (NF-B) activation. Additionally, acetoside partially reversed the dysbiosis of the gut microbiome in colitic mice, which was evident in the altered gut microbiome structure and the enrichment of helpful bacteria (_Akkermansia muciniphila_ and _Bacteroides thetaiotaomicron_) [25].