## 4 Discussion  
The results of the qualitative composition analysis of the dried biomass extracts of in vitro callus, cell suspension, and root cultures, showed that the main BAS of the medicinal plant _Rhaponticum arthamoides_ are 2-deoxy-5,20,26-trihydroxyecdyson, 5,20,26-trihydroxyecdyson 20,22-acetonide, 2-deoxy-5,20,26-trihydroxyecdyson 20,22-acetonide, 20,26-dihydroxyecdyson 20,22-acetonidecdyson and 20,22-acetonide. Stereochemistry for corresponding isomers of ecdysone was determined by the value of the spin-spin constant; for compound 4 and 5, value \(J\) increased, and this fact and conclusion led to the conclusion about the diastereomeric nature of the obtained compounds. We also took into account the values of the spin-spin interaction constant of the methylene group and protons of the cyclopentanoperhydrophenanthrene system. In this case, a decrease in the value of the spin-spin interaction constant was observed, which indicates the trans-configuration of methylene groups. In the side chain, a close value of the spin-spin interaction and chemical shift constants, with already known compounds, was found. From this, an assumption was made about a similar character of stereoisomerism for the obtained compounds. In the root culture of _Leuzea carthamoides_, [PERSON] et al. [33] found three new ecdysteroids (polypodine B 20,22-acetonide, 20-hydroxyecdysone 2,3; 20,22-diacetonide and  
Figure 2: Formulas of compounds present in the extract of callus, cell suspension, and root cultures of _Rhaponticum arthamoides_, identified by NMR spectra (the formula number corresponds to the peak number). isovitexiron together with 20-hydroxycytosone, 20-hydroxycytosone 2,3-acetonide, 20-hydroxycedysone 20,22-acetonide, ajugasterone C, macroistore A and polypodyne B). The described data confirm the presence of ecdysteroids in the _Rlaponticum earthquakes_ root cultures. The molecular structure of each compound was determined by NMR spectroscopy and HRMS analysis. A method for the isolation of an individual compound with high brightness and selectivity, and subsequent identification by NMR spectra, which characterize the magnitude of the chemical shift signal and the multiplet structure, is described. The BAS profile of callus, cell suspension, and root cultures of _Thevetia peruviana_ (an ornamental shrub growing in many tropical regions of the world) is described [34]. The biologically active compounds of this plant have unique characteristics and can be used as pharmaceutical substances for drugs under development. The research objects were the 50% aqueous ethanol and ethyl acetate extracts. The work used thin-layer chromatography and standard chemical tests for phytochemical analysis. High-performance liquid chromatography was used to analyze the phenolic chemical profile. The total amount of phenolic and flavonoid, the total amount of cardiac glycosides, and the total antioxidant activity in callus, cell suspension, and root cultures, were determined. All the samples under study had common biological activity; antioxidants, amino acids, alkaloids, flavonoids, phenols, cardiac glycosides, leukoanthocyanidins, triterpenes, and sugars were found in their composition. Dihydroquercetin, which has anticancer activity, has been identified using high-performance liquid chromatography. The presented results demonstrate the usefulness of _T. peruviana_ callus, cell suspension, and root cultures for the production of valuable pharmaceutical compounds. The data described in [34] confirm the high accumulation of flavonoids and ecdysteroids in in vitro callus, cell suspension, and root cultures of medicinal plants. In [35], ginseng callus, cell suspension and root cultures, and their extracts (alcohol content 30-70%), were studied. Organic compounds were determined by thin-layer chromatography. For each plant, quercetin, magneferin, luteolin, rutin, quercetin-2-D-glucose, malvidin, caffeic, cinnamic, ferulic, and sinapic acids were identified.