## 3 Results
### Characterization of Bee Pollen by Physico-Chemical Analyses  
Physico-chemical analyses were performed on multifocal and unifloral bee pollen samples (containing >30 g necessary for the analyses) isolated from the pooled samples for overall physico-chemical analyses. Bee pollen collected by the honey bees in CR contained six multifloral bee pollen samples (coded as mCR1-mCR6) and nine unifloral bee pollen samples, in MR contained six multifloral bee pollen samples (coded as mMR1-mMR6) and nine unifloral bee pollen samples, while in AR contained four multifloral bee pollen  
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samples (coded as mAR1-mAR4), and three unifloral bee pollen samples. The unifloral bee pollen samples were denoted as corresponding plant species. The results of the physico-chemical analyses are presented in Table 2.  
The moisture content in collected multifocal bee pollen samples from CR ranged from 15.01% to 22.40% and the ash content from 1.75% to 2.80%. The protein content ranged from 14.73% to 23.19%, total lipids from 8.74% to even 18.36%, which differed significantly from the other multifocal bee pollen samples from CR. Dominant sugars in analyzed bee pollen were monosaccharides fructose (13.99-20.05%) and glucose (8.92-15.87%), while the most represented disaccharides were sucrose (5.87-22.04%), maltose (1.56-3.85%), melezitose (0.27-1.54%), and raffinose (0.02-0.23%). The unifloral bee pollen of _Viburnum_ spp. contained the lowest moisture content (13.80%) while the highest value was observed in _T. officinal_ bee pollen (21.40%). The ash content ranged from 1.15% in _T. officinal_ to 3.08% in _C. sanguinea_ bee pollen sample. The highest protein content was determined in _A. hipprocstanum_ bee pollen (27.26%), and the lowest in _T. officinal_ (14.02%). Conversely, _T. officinal_ bee pollen contained the highest total lipid content (19.04%), while the lowest was determined in _A. hipprocastanum_ bee pollen (6.20%). Fructose ranged from 10.87% in _A. hipprocastanum_ bee pollen to 25.65% in _P. rhoets_ bee pollen, the content of glucose in _A. hipprocastanum_ bee pollen was 8.96%, and 18.80% in _T. officinal_ bee pollen, while sucrose ranged from 1.62% in _T. officinal_ bee pollen to 20.33% in _P. spinosa_ bee pollen. The highest value of other less represented sugars was determined for maltose in _T. officinal_ bee pollen (4.33%), and _J. regia_ (2.79%) bee pollen. The moisture content in multifocal bee pollen samples from MR ranged from 13.78% to 22.21%, the ash content from 1.84% to 2.89%, the protein content from 15.59% to 23.29%, and the total lipids from 6.13% to 14.57%. Sugar with the highest content was fructose (19.88%), followed by glucose with 18.70% and sucrose with 15.85%. Observing the values of unifloral bee pollen samples from MR, the lowest moisture content was found in _P. spinosa_ (11.30%), and the highest in _Q. pubescens_ samples (26.48%). The ash content ranged from 1.18% in _T. officinal_ bee pollen to 3.81% in _P. avium_ bee pollen. Proteins were the second largest constituent in the bee pollen after sugars. In _P. tanaceifolia_, bee pollen proteins were found with the content of 26.32%, and the lowest content was recorded in the bee pollen of _T. officinal_ (13.90%), which is similar to the value obtained for bee pollen from CR. The highest total lipid content was determined in _T. officinal_ bee pollen (17.53%), and the lowest in _F. vulgaris_ bee pollen (4.51%). The most represented sugar was fructose in _P. tanaceifolia_ bee pollen (24.28%), as well glucose (18.49%) and sucrose in _P. spinosa_ bee pollen (18.68%), while other sugars ranged from 0.05% for melezitose in _Q. pubescens_ bee pollen to 5.48% for maltose in _M. sativa_ bee pollen. The moisture content in multifocal samples from AR ranged from 14.18% to 22.84% and the ash content from 2.06% to 2.49%. The proteins were represented in the range of 17.21-21.19%, while the total lipids ranged from 8.52% to 10.80%.