## 6 Significance of Chemical and Biological Agents Encapsulation in Sustainable Agriculture
It is important to mention that the synergistic action of chemical and biological (biocon control) agents may improve their activity, and investigation of their relationship is of crucial importance for developing efficient products [119]. Figure 3: Macro photograph of _Trichoderma viride_ in a standard Petri dish and microphotograph (optical/ fluorescence microscope) of _Trichoderma viride_ stained with Rhodamine 123 mitochondria-specific fluorescent dye. \begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline
**Biological Agent** & **Particle Type** & \multicolumn{2}{c}{**Encopsulation**} & \multicolumn{2}{c}{**Storage**} & **Results** & **Application/Purpose** & **Literature** \\ \hline _Trichedrara supremum_ & - calcium-alignate & - & ionic gelation & - & fresh beads and & - & improving the & shelf life of \\ BRM-29104 conidia & - & beads & - & 2\% sodium & & freeze-dried beads & - & freeze-drying and cold storage & - & biocontrol agent \\ BRM-29104 conidia & - & size after & alginate & at 8 °C and in an & & maintains the viability of & - & to fight plant \\ microalgae & freeze-drying was & - & 0.2 mol dm\({}^{-3}\) & oven at 25 and 35 & & biocontrol agent & - & diseases and \\ microsatellite & \(2.5\pm 0.2\) mm & CaCl\({}_{2}\) & - & \({}^{\circ}\)C for 120 days & & & promote plant \\ \hline _Metrahizium brunnunnunn_ & - calcium & - & ionic gelation & - & calcium-alignate beads & - & entomopathogenic & fungi in \\ CaSH, CSH1, CSH11, CSH11 & CSH11 & CSH11 & - & alginate, 10\% & - & drying and storage & function as microthermometer & extract-and-kill \\ and CSH10F & - & supplemented & native granular & stability still needs & - & _Metrahizium_ spp. blastospores & formulation & \\ blastospores and & with yeast & corn starch, 1\% & & - & encapsulated blastospores & strategies as an & \\ _Metrahizium pempligi_ & - & beads size unknown & inactivated and & to be determined & maintained virulence against & option for \\ Xlc blastospores & (spring diameter & ground yeast & & tick (_Invics riting_ nymphs) & arthropod pest & \\ Xlc blastospores & \(2.1\times 0.8\) mm) & - & 2.0\% CaCl\({}_{2}\) & & & control & \\ \hline \multirow{6}{*}{_Apergillus flavus H4-5 spores_} & - calcium-alignate & - & ionic gelation & - & porous honeycomb structure - & the beads based on the semi- \\  & & isotropy/day/sample) & - & ionic gelation & - & sustainable and controllable & interpenetratingrating \\ \cline{1-1}  & & hydrogel beads & alginate, 10\% & - & alginate with good & network hydrogel \\ \cline{1-1}  & - & supplemented & starch, 1\% poly(N\)- & - & no shelf-like was investigated & - & thermosensitivity of beads & with fashion could \\ \cline{1-1}  & - & with kaolin & isopropylacrylamide, & - & is investigated & - & kaolin supplementation & serve as carriers of \\ \cline{1-1}  & - & dry bead diameter & 1-3\% kaolin & & - & results in a slow-release before & biocontrol agents \\ \cline{1-1}  & \(1.93\pm 0.08\) to \(2.63\) & - & 2\% CaCl\({}_{2}\) & & peanut lowering and rapid & in the early \\ \cline{1-1}  & \(\pm 0.12\) mm & & & release of biocontrol agents & flowering stage of \\ \cline{1-1} \cline{2-10}  & & & & after flowering begins & the peanut & \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Most recent (2017–2020) examples of biocontrol fungal agent encapsulation in biopolymer-based particles and its important outcomes.