## 3 Results
### Nonclassical Proteins Relevant to Virulence and Resistance
#### 3.6.1 Differences between the PL8 h and PL96 h Surfaceomes  
Proteins specific to PL96 h cells or more abundant (4- to 80-fold) on PL96 h than PL8 h cells (Table S5) included the fatty acid degradation enzymes (acetyl-CoA C-acyltransferase/FadA, long-chain fatty acid-CoA ligase/FadE, glutaryl-CoA dehydrogenase/FadD propionate CoA-transferase/FadX), TCA cycle (2-oxoglutarate dehydrogenase/2-OGDH, aconitase/AcnA, malate \begin{table}
\begin{tabular}{l c c c c} \hline  & **Ar. No.** & **Varience Factor Adhesion** & **Mr.\({}^{\bullet}\)** & **pI\({}^{\bullet}\)** & **Gr.\({}^{\bullet}\)** \\ \hline WP\_00094516.1 & HlgAB subunit A & 34.9 & 10.1 & \(-\)0.6 \\ WP\_001056917.1 & HlgAB/FilgC subunit B & 36.8 & 9.7 & \(-\)0.7 \\ WP\_00959617.1 & Gamma-morphism subunit B & 38.7 & 9.0 & \(-\)0.8 \\ WP\_00669728.1 & Protein MAF-domain containing & 76.8 & 10.6 & \(-\)0.5 \\ WP\_00028713.1 & Immunoglobulin G-binding–SpA & 54.7 & 5.4 & \(-\)1.1 \\ WP\_00027567.1 & Immunoglobulin binding––SpA & 50.2 & 9.9 & \(-\)1.0 \\ WP\_00071398.1 & Undracracracractional leukocyte & 40.5 & 9.9 & \(-\)1.1 \\ WP\_00071395.1 & Staphylococcal secretory SasA2 & 29.6 & 9.1 & \(-\)0.8 \\ WP\_000495296.1 & Clumping factor B CitIns & 99.0 & 3.6 & \(-\)1.2 \\ WP\_0072078.1 & Clumping factor A/CIA & 101.3 & 3.4 & \(-\)1.1 \\ WP\_00071265.1 & Immunoglobulin antigen IsaA & 24.2 & 6.6 & \(-\)0.3 \\ WP\_00170796.1 & Immunoglobulin antigen IsaB & 19.5 & 10.0 & \(-\)0.5 \\ WP\_00076972.1 & Peptidesopeptide YTEB & 21.3 & 5.1 & \(-\)1.1 \\ WP\_00082534.1 & Med ALS transporter & 30.5 & 9.5 & \(-\)0.3 \\ WP\_00103857.1 & \(\gamma\)-nucleides, lipophilic protein (F4) family & 33.3 & 10.1 & \(-\)0.8 \\ WP\_00174521.1 & Bifunctional autolys–Atta & 137.9 & 10.1 & \(-\)0.6 \\ WP\_01107274.1 & Autolys–Sel & 36.0 & 10.0 & \(-\)0.4 \\ WP\_0295175.1 & Extracellular matrix-binding–Eth8 & 1087.3 & 6.1 & \(-\)0.7 \\ WP\_03841312.1 & Fibronectin-binding–FabA & 114.1 & 4.2 & \(-\)0.9 \\ WP\_0384136.1 & Collagen adhesion & 1330.0 & 6.1 & \(-\)1.0 \\ WP\_00151905.1 & Cell wall-anchored protein SasF & 70.1 & 8.9 & \(-\)0.7 \\ WP\_0003494.1 & MSCNAM adhesion SdrD & 1452.4 & 4.0 & \(-\)1.0 \\ WP\_07670283.1 & MSCRAM adhesion SdrD (partial) & 1049.4 & 4.3 & \(-\)0.9 \\ WP\_07670284.1 & MSCRAM adhesion SdrD & 88.5 & 4.6 & \(-\)1.0 \\ WP\_001041875.1 & Hemat uptake protein–IsIsdR & 72.6 & 9.5 & \(-\)0.9 \\ WP\_00728052.1 & Matrix binding-hesin Imp & 38.5 & 10.5 & \(-\)0.5 \\ WP\_000782130.1 & Foldage protein Prsa & 35.6 & 9.8 & \(-\)0.9 \\ WP\_00129090.1 & ABC transporter CntA & 60.0 & 9.3 & \(-\)0.8 \\ WP\_0002180.1 & Catabolite control–CpxpA & 36.0 & 6.4 & \(-\)0.2 \\ WP\_00037654.1 & Manganese transport–MatC & 34.7 & 9.1 & \(-\)0.7 \\ \hline \end{tabular} \({}^{\text{a}}\) Mr, molecular mass/kDa; pl, isoelectric point; Gr, GRAVY index. PL, planktonic; EDBF and SDBF, biofilms initiated with exponential (2 h) and stationary phase cells (72 h), respectively. Color code, low (blue) and high (yellow) protein abundances. When cells are grey, identification is below detection limit.  
\end{table} 
Table 1: Abundance patterns of selected classical surface proteins on planktonic and biofilm cells. dehydrogenase/quinone/MQD), Fe-S repair (SufB), urea cycle (argininosuccinate synthase/AsS, argininosuccinate lyase/AsL, arginine deaminase/ArcD), oligopeptide uptake (OppD), peptidases (PepP, PepM, PepS and PepF, ClpP), hydrolases, stress chaperones (DnaK, DnaJ, GrpE, GroES, DnaJ, ClpB), ROS scavenging enzymes (superoxide dismutases SodM, SDR family NAD(P)-dependent oxidoreductase, catalase, alkyl hydroperoxide reductase subunit F/AhpF), and a DNA translocase (FtsK). Glycolytic moonlighters such as the phosphoenolpyruvate carboxylase (PCK) and glucose-6-phosphate dehydrogenase (GPD) were more abundant on PL96 h than PL8 h cells. From these identifications the Fad-proteins are encoded by the _faABDEX_, and the operon and stress response proteins through the _dnal-dnal-grpE_ operon.