## 3 Results and Discussion
### Method Validation of the Developed HPLC Analytical Method
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Analyte**} & \multirow{2}{*}{\
\begin{tabular}{c} **Measured** \\ **Amount (\(\mu\)g/mL)** \\ \end{tabular} } & \multirow{2}{*}{
\begin{tabular}{c} **Found Amount** \\ **(\#g/mL)** \\ \end{tabular} } & \multirow{2}{*}{**Recovery (\%)**} & \multirow{2}{*}{**SD**} & \multirow{2}{*}{**RSD (\%)**} \\ \hline
**1** & 1.00 & 1.01 & 100.81 & 1.73 & 1.72 \\
**1** & 2.00 & 1.99 & 99.41 & 0.94 & 0.95 \\  & 4.00 & 3.93 & 98.23 & 1.17 & 1.19 \\  & 2.00 & 2.07 & 103.52 & 0.98 & 0.94 \\
**2** & 4.00 & 3.99 & 99.70 & 1.00 & 1.00 \\  & 8.00 & 8.27 & 103.36 & 0.59 & 0.57 \\  & 1.00 & 1.02 & 101.52 & 1.50 & 1.48 \\
**3** & 2.00 & 1.99 & 99.58 & 0.36 & 0.36 \\  & 4.00 & 3.96 & 99.07 & 0.82 & 0.83 \\  & 4.00 & 4.04 & 100.90 & 2.80 & 2.77 \\
**4** & 10.00 & 9.92 & 99.24 & 2.97 & 2.99 \\  & 20.00 & 19.41 & 97.03 & 1.13 & 1.17 \\  & 2.00 & 1.98 & 99.19 & 1.86 & 1.88 \\
**5** & 5.00 & 4.97 & 99.45 & 1.11 & 1.12 \\  & 10.00 & 9.91 & 99.13 & 1.40 & 1.41 \\  & 1.00 & 1.00 & 100.31 & 1.24 & 1.24 \\
**6** & 2.00 & 2.01 & 100.43 & 1.48 & 1.47 \\  & 4.00 & 4.04 & 101.04 & 0.85 & 0.84 \\  & 1.00 & 1.01 & 100.56 & 1.79 & 1.78 \\
**7** & 2.00 & 1.99 & 99.73 & 0.67 & 0.67 \\  & 4.00 & 3.91 & 97.79 & 0.44 & 0.45 \\  & 1.00 & 1.04 & 104.04 & 2.36 & 2.27 \\
**8** & 2.00 & 1.96 & 98.25 & 2.56 & 2.60 \\  & 4.00 & 3.97 & 99.32 & 1.50 & 1.51 \\ \hline \hline \end{tabular}  
\({}^{\mathrm{a}}\)\(y\): peak area of compounds; \(x\): concentration (\(\mu\)g/mL) of compounds.  
\end{table} 
Table 2: Extract recovery test of compounds **1–18** in the developed analytical assay. \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multirow{2}{*}{**Analyte**} & \multicolumn{2}{c}{**Measured**} & \multicolumn{2}{c}{**Found Amount**} & \multirow{2}{*}{**Recovery (\%)**} & \multirow{2}{*}{**SD**} & \multirow{2}{*}{**RSD (\%)**} \\  & **Amount (\(\mu\)g/mL)** & & **(\(\mu\)g/mL)** & & & \\ \hline \hline \multirow{4}{*}{**9**} & 1.00 & 1.00 & 99.65 & 1.40 & 1.41 \\  & 2.00 & 2.02 & 100.84 & 1.34 & 1.33 \\  & 4.00 & 4.06 & 101.62 & 0.84 & 0.83 \\  & 5.00 & 5.13 & 102.63 & 2.36 & 2.30 \\
**10** & 12.50 & 12.79 & 102.29 & 1.23 & 1.20 \\  & 25.00 & 25.09 & 100.36 & 1.36 & 1.36 \\  & 2.00 & 1.97 & 98.37 & 2.86 & 2.90 \\
**11** & 5.00 & 4.96 & 99.16 & 1.60 & 1.61 \\  & 10.00 & 9.86 & 98.57 & 1.01 & 1.03 \\  & 3.00 & 2.96 & 98.74 & 0.97 & 0.98 \\
**12** & 7.50 & 7.42 & 98.89 & 0.82 & 0.83 \\  & 15.00 & 15.44 & 102.95 & 0.72 & 0.70 \\  & 1.00 & 1.01 & 100.80 & 1.49 & 1.47 \\
**13** & 2.00 & 2.03 & 101.35 & 0.54 & 0.53 \\  & 4.00 & 4.05 & 101.20 & 0.67 & 0.66 \\  & 1.00 & 1.01 & 100.61 & 1.83 & 1.82 \\
**14** & 2.00 & 2.03 & 101.74 & 0.90 & 0.89 \\  & 4.00 & 4.14 & 103.59 & 0.88 & 0.85 \\  & 2.00 & 1.99 & 99.45 & 1.20 & 1.21 \\
**15** & 5.00 & 4.96 & 99.14 & 1.61 & 1.62 \\  & 10.00 & 9.82 & 98.25 & 1.79 & 1.82 \\  & 2.00 & 2.04 & 101.96 & 0.89 & 0.87 \\
**16** & 4.00 & 4.06 & 101.47 & 1.44 & 1.42 \\  & 8.00 & 8.04 & 100.53 & 1.64 & 1.63 \\  & 3.00 & 3.10 & 103.47 & 2.29 & 2.21 \\
**17** & 7.50 & 7.53 & 100.37 & 1.18 & 1.18 \\  & 15.00 & 14.99 & 99.90 & 0.34 & 0.34 \\  & 1.00 & 0.99 & 98.85 & 1.91 & 1.93 \\
**18** & 2.00 & 2.02 & 101.14 & 1.02 & 1.01 \\  & 4.00 & 4.10 & 102.45 & 0.57 & 0.56 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: _Cont._