## 3 Results
### In-Vitro Assays of \(L\). pubescens Antioxidant Activity  
In this study, among the organs assessed, we established that the leaves contain the high amounts of triterpenoids, total phenolic content, and total flavonoid content, and on the basis of these observation, we sought to determine the antioxidant ability of \(L\). _pubescens_ leaf extracts. The reducing power of a chemical compound is considered an important indicator for antioxidant screening, as it is indicative of electron transfer capacity. These reducers can promote the conversion of the Fe\({}^{3+}\)/ ferricyanide complex to the Fe\({}^{2+}\)/potassium ferrocyanide form, which thereby provides a basis for assaying antioxidant activity. In the present study, we assessed the reducing power activity of a crude \(L\). _pubescens_ ethyl acetate extract, which was compared with that of ascorbic acid (Figure 7A). We accordingly established that the extract has significant reducing power, as indicated by a concentration-dependent increase in the absorbance of the reaction mixture. Indeed, even at low concentrations, the antioxidant capacity of the extract was higher than that of ascorbic acid. An assessment of DPPH radical scavenging activity is one of the most efficient methods for screening the antioxidant activity of plant extracts, and we accordingly observed that the antioxidant activity of the ethyl acetate extract of \(L\). _pubescens_ was comparable to that of ascorbic acid used as a standard and was concentration dependent, with a highest scavenging activity of 60.46 \(\pm\) 0.9% being obtained using the extract at a concentration of 100 \(\upmu\)g/mL (Figure 7B). Figure 7: The antioxidant activity of an ethyl acetate extract of _Lavandula pubescens_ leaves. (**A**) Reducing power activity; (**B**) 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity; (**C**) Hydrogen peroxide scavenging activity; (**D**) Superoxide radical scavenging activity; (**E**) Hydroxyl radical scavenging activity. The blue bars represent values obtained using an ethyl acetate extract of \(L\). _pubescens_ leaves and the red bars are values obtained using ascorbic acid. Hydrogen peroxide can rapidly cross cell membranes, and having entered the cytoplasm may react with Fe2+ ions and possibly Cu2+ ions to form hydroxyl radicals, which are potential origins of many of its toxic effects. We found that the H2O2 radical scavenging activity of the _L.__pulsecens_ ethyl acetate extract was concentration dependent and comparable to that of the ascorbic acid standard, with the highest activity of 57.46% being obtained using the extract at 100 \(\mathrm{\SIUnitSymbolMicro g}\)/mL, which compares with the activity of 76.52% obtained with ascorbic acid used at a concentration of 80 \(\mathrm{\SIUnitSymbolMicro g}\)/mL (Figure 7C). Superoxide radical scavenging activity was assessed by calculating the percentage inhibition of superoxide radical generation by the _L.__pulsecens_ ethyl acetate extract compared with that obtained using the standard ascorbic acid. At an extract concentration of 80 \(\mathrm{\SIUnitSymbolMicro g}\)/mL, we obtained a superoxide radical scavenging activity of approximately 50%, which increased to 62.6% when using the extract at a concentration at 100 \(\mathrm{\SIUnitSymbolMicro g}\)/mL. Comparatively, the highest scavenging activity using ascorbic acid was 80.07% obtained using a concentration of 100 \(\mathrm{\SIUnitSymbolMicro g}\)/mL (Figure 7D). The hydroxyl radical (\(\bullet\)OH) is particularly reactive in biological systems and has been associated with highly damaging species in free radical pathology and damaging biomolecules in living cells. These radicals combine with nucleotides in DNA, thereby promoting strand breakage and consequent carcinogenic, mutagenic, and cytotoxic effects.