# The Volatile Compounds Change during Fermentation of _Saccharina japonica_ Seedling
## 3 Results
### Qualitative and Quantitative Analysis of Volatile Compounds before and after Fermentation
\begin{table}
\begin{tabular}{c c c c c c c c c c c} \hline \hline \multirow{2}{*}{**NO.**} & \multirow{2}{*}{**RT/min**} & \multirow{2}{*}{**Compounds**} & \multirow{2}{*}{**RI \({}^{\ast}\)**} & \multirow{2}{*}{**RI \({}^{\ast}\)**} & \multirow{2}{*}{**RI \({}^{\ast}\)**} & \multirow{2}{*}{**Fragment**} & \multirow{2}{*}{**Identification**} & \multirow{2}{*}{**Standard Curves**} & & \multicolumn{2}{c}{**Concentration (\(\upmu\)g/g)**} \\ \cline{6-10}  & & & & & & & & & & & **SJS** & **FSJS** \\ \hline
7 & 30.28 & 1.3-Octadiene & 1692 & 1692 & 55 97 238 & MS, RI, Std & Y = 6.231025X \(-\) 0.045231 & 0.999 & 7.8 \(\pm\) 0.2 & - \\
8 & 34.69 & D-limonene & 1729 & 1728 & 68 93 136 & MS, RI, Std & Y = 6.131623X \(-\) 3.776805 & 0.997 & - & 8.3 \(\pm\) 3.5 \\
9 & 37.83 & 1-Undechere & 1791 & 1791 & 55 69 97 & MS, RI, Std & Estimated & - & - & 3.2 \(\pm\) 0.3 \\
10 & 38.23 & 4-Methyl-1 & & & & & & & & & \\  & & & & & & & & & & & \\
11 & 40.28 & Heptadecene & 1821 & 1822 & 55 97 238 & MS, RI, Std & Y = 4.965865X \(-\) 0.552135 & 0.998 & 31.0 \(\pm\) 3.1 & - \\  & & & & & & & & & & & \\  & & & & & & & & & & & \\
1 & 12.52 & Heey1y acetate & 1004 & 43 56 84 & MS, RI, Std & Y = 0.8865232 & \(-\) 2.443578 & 0.999 & 5.1 \(\pm\) 1.3 & - \\
2 & 16.87 & Phenethyl acetate & 1283 & 1281 & 43 91 105 & MS, RI, Std & Y = 3.412776X \(-\) 0.742351 & 0.999 & - & 8.7 \(\pm\) 2.4 \\
3 & 18.12 & Heey1 butyrate & 1282 & 1284 & 33 61 92 & MS, RI, Std & Y = 5.120312X \(-\) 0.635227 & 0.996 & 0.6 \(\pm\) 1.5 & - \\
4 & 18.43 & Ethly ciprate & 1289 & 1287 & 43 58 97 & MS, RI, Std & Y = 5.123721X \(-\) 0.64124 & 0.998 & - & 2.3 \(\pm\) 1.2 \\
5 & 23.31 & Ethyl Laurate & 1724 & 1725 & 45 63 112 & MS, RI, Std & Y = 3.26584X \(-\) 0.536241 & 0.999 & - & 3.6 \(\pm\) 1.4 \\
6 & 32.03 & Methyl palmitate & 1827 & 1827 & 43 60 102 & MS, RI, Std & Y = 4.776543X \(-\) 3.156005 & 0.993 & 65.3 \(\pm\) 8.2 \({}^{\circ}\) & 44.3 \(\pm\) 10.7 \\  & & & & & & & & & & \\  & & & & & & & & & & & \\
1 & 9.62 & 2-Pentiyfuran & 988 & 987 & 81 138 & MS, RI, Std & Y = 1.063317X \(-\) 0.033126 & 0.994 & 18.9 \(\pm\) 3.1 & - \\
2 & 18.81 & 2-Methyl-2 & & & & & & & & & \\  & & & & & & & & & & & \\  & & & & & & & & & & & \\  & & & & & & & & & & & \\
3 & 22.16 & Dimethylnaphthalene & 1503 & 1502 & 141 155 156 & MS, RI & Estimated & - & 0.4 \(\pm\) 0.5 & - \\ \hline \hline \end{tabular} Note: SJS refers to unfermented _S. japonica_ seedling; FSJS refers to fermented _S. japonica_ seedling; RT represents the retention time of aroma compounds on the Rtx-SMS column. RI \({}^{\ast}\) was calculated based on the retention time of C\({}_{8}\)–C\({}_{20}\). RI \({}^{\ast}\) was obtained from the web: [[http://webbook.nist.gov/chemistry/](http://webbook.nist.gov/chemistry/)]([http://webbook.nist.gov/chemistry/](http://webbook.nist.gov/chemistry/)) (accessed on 20 March 2020). Identification method: MS, mass spectrum comparison using (NIST11, NIST11s, FFNSC1.3) library; RI: retention index in agreement with literature value; Std: validated with authentic standards. Estimations suggest that the unquantified volatile compounds were treated as equivalent units of internal standard added to a sample matrix. \({}^{\circ,d}\) Signal intensities are shown as mean \(\pm\) standard deviation (\(n=3\)). Significant differences were performed using Tukey’s test (\(p<0.05\)) in SPSS 19.0.  
\end{table} 
Table 1: _Cont._