## 2 Results
### Proteome Profiling of Macro-Mutant Lines
#### 2.2.2 Identification and Expression Patterns of Differentially Expressed Proteins of Mutant lines
Compared to the control, this protein was up-regulated in the leaves of the HY1, HY3, MHY4, NY3, LY3, and VLY2 mutants, and down-regulated in MHY2, LY1, and VLY3 (Table 5). Spot #1268 has been identified as a Tubulin alpha-6 chain (TUA6). The MW and pI of this spot were found to be 49,537 Da and 4.93, respectively. This protein is present in the cytoskeleton and involved in microtubule cytoskeleton organization. The expression of this protein was upregulated in the leaves of the HY1 and MHY2 mutant lines of _N. sativa_ when compared with the control. In the mutants HY3, MHY4, and NY3, the expression of this protein was equal to that of the control. There was, however, down-regulation in the expression of this protein in the leaves of LY1, as well as VLY2 and VLY3. Spot #7315 has been identified as eukaryotic translation initiation factor 3, subunit I. The MW and pI of this spot were found to be 36,388 Da and 6.50, respectively. This spot is present in the cytoplasm and takes part in the synthesis of protein. This protein was upregulated in the mutants HY1, MHY2, and LY3 when compared to the control. In other mutant lines, down-regulation in the expression of this protein was observed in comparison to the control. Spot #2401 has been identified as allene oxide cyclase 3. This protein is present in the chloroplast and is involved in jasmonic acid biosynthesis. This protein was upregulated in the MHY2, LY1, and VLY3 mutants when compared with the control. In other mutants, the expression of this protein was equal to that of the control. Spot #3001 has been identified as deoxyuridine 5\({}^{\prime}\)-triphosphate nucleotid hydrolase. This protein is involved in nucleotide metabolism and present in the cytoplasm. This protein was upregulated in HY1, MHY4, and LY3 mutants when compared with the control. In all the other mutants, the expression of this protein was equal to that of the control. Spot #3909 has been identified as the GTP-binding nuclear protein, Ran-1. The MW and pI of this spot were found to be 25,275 Da and 6.38, respectively. The expression pattern of this protein in the leaves of the mutants was similar to that of spot #6301. Spot #4006 is the polycomb group protein, FIE1. The MW and pI of this protein are 52,100 Da and 7.55, respectively. Compared to the control, the expression of this protein was upregulated in the HY1 and MHY2 mutant lines, when compared to the control. In all the other mutant lines, this protein was down-regulated, except in HY3 and MHY3, where its expression was equal to that of the control plant. Spot #4518 has been identified as the transcription factor, PRE1. This protein was up-regulated in the HY1 and MHY2 mutant lines, but down regulated in the NY3, LY1, LY3, VLY2, and VLY3 mutant lines when compared with the control. In the HY3 and MHY4 mutant lines, the expression of this protein is equal to that of the control. Spot #4900 has been identified as geranyl diphosphate synthase. Expression of this protein was up-regulated in the NY3, LY3, and VLY3 mutant lines, but remained equal to the control (Tables 4 and 5). \begin{table}
\begin{tabular}{c c c c c c c c c c c} \hline \hline
**Spot** & **Protein** & **MASCOT** & **Nominal** & **pI** & **Sequence** & **Protein Name** & **Protein Function** & **Sub-Cellular** & **Pathway/Biological** & **Accession** \\
**No. ** & **View** & **Score** & **Mass (Da)** & **pl** & **Method (\%)** & **Protein Name** & **Protein Function** & **Location** & **Process** & **No. ** \\ \hline
1011 & RBL\_ERYCG & 148 & 50841 & 6.22 & 65 & \ \begin{tabular}{c} Ritualose bisphosphate \\ carboxylase large chain \\ \end{tabular}  & \begin{tabular}{c} Carboxylation of \\ D-ribulose \\ 1.5-bisphosphate \\ \end{tabular}  & Chloroplast & Photosynthesis & Q3348 \\ \hline
3401 & RCA\_MAZE & 119 & 48079 & 6.20 & 53 & \begin{tabular}{c} Ritualose bisphosphate \\ carboxylase/oxygenase \\ \end{tabular}