# Role of Exposure on the Microbial Consortium on Historical Rural Granite Buildings
## 3 Results and Discussion
### Next Generation Sequencing
In terms of biodeterioration, some consideration should be given to _Acidicalus organivorans_ as its sulfide-reducing nature contributes to mineral dissolution [55]. Figure 5: Relative abundances (%) of bacterial and fungi group for each phylum (**A**) and class (**B**). Only species with a frequency greater than 0.001% were considered in the analysis. \begin{table}
\begin{tabular}{c c c c c c c c c c c c c} \hline \multirow{2}{*}{**DOMAIN**} & \multirow{2}{*}{**PHYLUM**} & \multirow{2}{*}{**CLASS**} & \multirow{2}{*}{**ORDER**} & \multicolumn{4}{c}{**NORTH (\%)**} & \multicolumn{4}{c}{**WEST (\%)**} & \multirow{2}{*}{**GENUS**} & \multirow{2}{*}{**SPECICS**} \\ \cline{4-11}  & & & & **SMV** & **BAR** & **ROC** & **SMV** & **BAR** & **ROC** & & & & & \\ \hline \multirow{11}{*}{**BACTERIA**} & \multirow{11}{*}{Proteobacteria} & \multirow{11}{*}{Proteobacteria} & \multirow{11}{*}{14.4} & \multirow{11}{*}{6.3} & \multirow{11}{*}{29.4} & \multirow{11}{*}{10.5} & \multirow{11}{*}{10.6} & \multirow{11}{*}{10.5} & \multirow{11}{*}{10.6} & \multirow{11}{*}{_Resonormous_} & \multirow{11}{*}{_Resonormous_} & \multirow{11}{*}{_Resonormous_} \\  & & & & & & & & &  
\begin{tabular}{c c c c c c c c c c c c} \multicolumn{11}{c}{Table 2: _Cont._} & & & & & & & & & & \\ \hline
**Domain** & **PHYLUM** & **CLASS** & **ORDER** & \multicolumn{3}{c}{**NORTH (\%)**} & \multicolumn{3}{c}{**WEST (\%)**} & \multicolumn{1}{c}{**GENUS**} & \multicolumn{1}{c}{**SPECIES**} \\ \cline{4-12}  & Deinococcus & Deinococci & Turepareales & 0.2 & 0.2 & / & 2.4 & 2.9 & / & _Turepare_ & _Turepare_ & _Turepare_ & _Turepare_ \\ \cline{4-12}  & Thermus & Spartobacteria & Chinoobacterales & / & 1.5 & 0.2 & / & 2.3 & 1.0 & _Chineobacter_ & _Chineobacter_ & _Chineobacter_ & _Heas_ \\  & Cyanobacteria & Cyanophyceae & Nostocales & / & / & / & / & / & 0.2 & _Lerialopsis_ & _Lerialopsis_ & _Lerialopsis_ \\ \hline \multirow{12}{*}{**BACTERIA**} & \multirow{2}{*}{Cleanorocytes} & \multirow{2}{*}{Cleanorocytes} & \multirow{2}{*}{6.7} & \multirow{2}{*}{23.0} & \multirow{2}{*}{2.1} & \multirow{2}{*}{52.9} & \multirow{2}{*}{26.1} & \multirow{2}{*}{42.2} & \multirow{2}{*}{_Leconia orella_} \\  & & & & & & & & & & & & \\ \cline{4-  
Within the order Caulobacterates, the species _Brevundimonas albigilva_ was found to colonize the N-facing walls of the BAR and ROC churches (1.4 and 2.8) _Brevundimonas variabilis_ only appeared on the N-facing wall of the ROC church (11%). _Phenylobacterium aquaticum_ was found on the N-facing walls of the SMV and ROC churches (1 and 4.6%), as well as on the W-facing wall of the ROC church (1.2). Both genera have previously been found colonizing other monuments, especially on sandstone in polluted areas, as they can survive in such environments due to their capacity to degrade hydrocarbons and use them as a carbon source [56; 57; 58]. Members of the order Rhizobiales mainly appeared on the ROC church. More specifically, _Salitarinutosa rosse_ was found on both the N- and W-facing walls of this church (3.2 and 1%), as well as on the N-facing wall of the BAR church (5.8%). In addition, _Baudlia litorialis_ and _Roseiarcus fermentans_ were also found on the ROC church, the former on both N- and W-facing walls (4 and 1.5%) and the latter on the N-facing wall (4.1%). This species produces bacteriochlorophyll \(a\), and carotenoids, which have been reported to cause an aesthetic impact on the stone where the species occurs [59]. Examples of the genus _Aureiomonas_ were only found on W-facing walls, in all three churches (approx. 2%). This order is commonly associated with the roots of vascular plants and its occurrence may indicate an advanced level of colonization on the facades (plants rooted in the rock) but may also be due to the influence of endophytes from neighboring forest areas deposited on walls by winds. It has been found to colonize a red sandstone monolithic statue of [PERSON] [53] in a region of China with a subtropical monsoon climate and a large presence of higher plants.