## 1 Introduction  
Tomatoes (_Lycopersicum esculent_) represent the most consumed vegetables worldwide grown for both fresh consumption and processing (mainly to make tomato paste), thus identifying two different industry segments. According to statistics [1], 182 million tons of tomatoes were globally grown in 2017, 25 million tons being produced in Europe. Forty percent of the European tomatoes are sold on the fresh market. Today, Italy represents the first major European producer (followed by Spain), capable of providing 6 million tons of product per year.  
In the last two decades, breeding programs and hybrid seed production have been addressed to enhance plant tolerance to abiotic and biotic stresses, fruit nutritional value, and fruit quality [2].  
[PERSON] [3] has reported that in the USA, as well as in most European, Asian, and Australian countries, the majority of tomato production (100% of fresh market and 80% of processing tomatoes) is obtained with hybrids [2]. Hybrid tomato cultivars are more frequently used than the open pollinated lines thanks to advantages such as high productivity, early maturation, high fruit uniformity, high fruit quality, and disease resistance. Tomato hybrid production often gives plants with desirable characteristics superior with respect to the native ones but, at the same time, the production of hybrid seeds is generally not easy and laborious [2].  
Tomato features such as taste and fragrance as well as healthful properties depend on the fruit chemical composition. For instance, sugars are responsible for different sweetness levels; organic acids are correlated with sourness; amino acids such as glutamate, glutamine, and aspartate enhance the bitterness [4; 5]; whereas glutamic acid seems to be correlated to umami taste [6].  
Among different analytical methods, high-resolution Nuclear Magnetic Resonance (NMR) spectroscopy is one of the most suitable approaches to monitor the composition of foodstuffs, owing to its ability to identify and quantify major and minor components without physical separation in the same experiment [7; 8]. The NMR metabolomic approach has been largely used to characterize tomatoes fruits and derived products. Besides the identification of principal components such as sugars, organic acids, amino acids, and minor components [9; 10; 11; 12], several studies have been carried out to determine carotenoid composition [13], degree of ripeness [14; 15; 16; 17], geographical origin [18], and sensory features [4; 5; 19]; to compare organic and conventional tomatoes [20]; and to evaluate genetic transformations [21; 22].  
Within the regional project titled \"eALIERB: an OPEN LAB to characterize and valorize foodstuffs and botanicals of Lazio region\", the chemical composition of hybrid tomatoes namely Bamano, DulceMiel, Sugarland, and King Creole cultivars together with the native Italian Fiaschetta di Fondi cultivar was investigated for the first time. These hybrid cultivars have been recently introduced in the Lazio region (Central Italy) aiming at expanding the regional agronomic fresh market with products characterized by peculiar sensorial and nutritional features useful for industries and customers. In this paper, the NMR-based characterization of Bamano, DulceMiel, Sugarland, and King Creole hybrid cultivars together with the native Italian Fiaschetta di Fondi cultivar was carried out to obtain and compare their metabolite profiles.