## 3 Results and Discussion
### Description of Jannaschia Sediminis sp. nov.  
Jannaschia Sediminis (se.di'mi.nis. L. gen. n. sediminis, of sediment). The cells of strain OS4T are oval, Gram-stain-negative, facultatively aerobic, non-motile, and approximate with a length of 0.8-2.0 um and a width of 0.5-0.8 um. The colonies on MA are circular, pink, and smooth and approximately (<1 mm) in diameter after 3 days of incubation at 25 \({}^{\circ}\)C. Growth occurs at 20-37 \({}^{\circ}\)C (optimal 25 \({}^{\circ}\)C), at pH 7.0-8.5 (optimal pH 8.0), in 0-5.0% (\(w/v\)) NaCl (optimal 2.0%). The cells are positive for oxidase and catalase activity. Positive results for the hydrolysis of alginate, casein and negative for hydrolyzing starch and tweens (20, 40, 60, and 80) were observed. Strain OS4T shows activities of alkaline phosphatase, esterase (C4) and esterase lipase (C8), lipase (C14), leucine arylamidase, valine arylamidase, cystine arylamidase, and acid phosphatase. In the carbon source oxidation test, positive results were obtained for sucrose. The major fatty acids are C18:1 \(\omega\)6c/\(\omega\)7c, C18:0, C10:30 OH, C20:1 \(\omega\)7c, and C16:0. The sole respiratory quinone is Q-10. The polar lipids consist of three phospholipids (i.e., phosphatidylcholine (PC), phosphatidylcholine), phosphatidylethanolamine (PE), phosphatidylglycerol (PG)), two unknown amino-lipids (i.e., aminolipids (AL) and aminophospholipid (APL)), and four unknown glycerolipids. The DNA G + C content of OS4T is 72.7 mol%. The type strain, OS4T (=KCTC 82508T = MCCC 1K03755T), was isolated from a marine sediment sample obtained from the Golden Bay of Weihai, China (E 122.057274, N 37.541204). The GenBank accession number for the 16S rRNA gene sequence of strain OS4T is MW559544, and the draft genome data have been deposited in GenBank under the accession number JAFCAR000000000.  
In addition, strain OS4T is resistant to a wide range of antibiotics (i.e., kanamycin, carbenicillin, neomycin, chloramphenicol, gentamycin, ofloxacin, oxytetracycline, streptomycin, ampicillin, tobramycin, tetracycline, and clindamycin). The wide range of antimicrobial resistant genes identified in OS4T highlights that those aquatic systems could potentially serve as a natural reservoir for ARGs and should be raising concerns about controlling the transfer and spread of antibiotic resistance from ocean non-pathogenic bacteria to pathogens.  
The following are available online at [[https://www.mdpi.com/article/10.3390/app12062883/s1](https://www.mdpi.com/article/10.3390/app12062883/s1)]([https://www.mdpi.com/article/10.3390/app12062883/s1](https://www.mdpi.com/article/10.3390/app12062883/s1)), Table S1: antimicrobial susceptibility of strain OS4T and the related _Jannaschia_ sAPLcies, Table S2: broad-spectrum profiles of ARG abundance predicted with OS4T whole genome, Figure S1: maximal likelihood phylogenetic tree of strain OS4T and other related species, Figure S2: the polar lipid composition of OS4T.  
**Author Contributions:** Conceptualization, S.S. and G.C.; methodology, S.S., L.Z., S.L. and G.C.; software, J.Z.; validation, S.S., L.Z., S.L. and J.Z.; formal analysis, S.S., L.Z. and S.L.; investigation, S.S. and L.Z.; writing--original draft preparation, S.S.; writing--review and editing, S.S., L.Z., S.L., J.Z. and G.C.; visualization, L.Z.; supervision, G.C.; project administration, G.C.; funding acquisition, G.C. All authors have read and agreed to the published version of the manuscript.  
**Funding:** This research was funded by the Shandong Provincial Key Research and Development Program, grant number 2019 CSF109007.  
**Institutional Review Board Statement:** Not applicable.  
**Informed Consent Statement:** Not applicable.  
**Data Availability Statement:** The draft genome data have been deposited in GenBank under the accession number JAFCAR00000000.  
**Acknowledgments:** We thank [PERSON] and his group members for the technical and resources support.  
**Conflicts of Interest:** The authors declare no conflict of interest.