intermedia_ seeds contain, next to Met-derived GSLs **73**, **95**, and **107**, also Phe-derived GSL, i.e., benzyl GSL (glucotropaeolin, **11**) [22]. Later report, using UHPLC-DAD-MS/MS and standards revealed in _I. umbelata_ next to **12**, **73**, and **95**, also Phe-derived GSL **11** and one Trp-derived 4-methoxyindol-3-ylmethyl GSL (4-methoxyglucobrassicin, **48**) [23]. \begin{table}
\begin{tabular}{l c c c c c c c c} \hline \hline \multirow{2}{*}{**Species**} & \multicolumn{8}{c}{**Aminoacid Precursor**} \\ \cline{2-9}  & & \multicolumn{3}{c}{**Met**} & **Phe** & **Trp** & **References** \\ \cline{2-9}  & **12** & **64** & **73** & **82** & **84** & **95** & **107** & **11** & **48** & \\ \hline _I. amara_ & & & & & & & & [18; 20; 24; 25; 26; 27] \\ _I. compacta_ & & & & & & & [20] \\ _I. crenata_ & & & & & & & [20; 26] \\ _I. gibrtalarica_ & & & & & & & [20] \\ _I. hybrida_ & & & & & & & [20] \\ _I. internedia_ & & & & & & [20] \\ _I. linifolia_ & & & & & & & [22] \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Distribution of the glucosionolates in plants belonging to the _Iberis_ genus investigated to date. [PERSON] et al. (2019) isolated essential oil from _Iberis amara_ seeds using various methods, including steam distillation, hydrodistillation, and ultrasound-assisted hydrodistillation. They have identified certain volatile compounds, specifically but-3-enyl ITC, 3-methylbutyl ITC, and butyl ITC. However, these compounds were not included in Table 1 because no standards were employed for their identification. They have tested the obtained volatile isolates for their cytotoxic effects on human colon cancer cell lines SW480 and HCT116, revealing inhibitory concentrations (IC\({}_{50}\)) in the range of 35-42 ug/mL and 63-70 ug/mL, respectively [28]. The hydroethanolic _I. amara_ extract, a popular phytomedicine for treating digestive problems, dramatically reduced the growth of HT-29 and T84 colon carcinoma cells, having IC\({}_{50}\) values of 6 and 9 ug/mL, respectively. The researchers also conducted experiments on two different cancer cell models, specifically PC-3 prostate cancer cells and MCF7 breast cancer cells. Similar to the previous findings for the two colon cancer models, the _I. amara_ extract exhibited an inhibitory effect on the proliferation of PC-3 and MCF7 cancer cells, with IC\({}_{50}\) values of 44 g/mL and 11 g/mL, respectively. In the case of HT-29 cells, the inhibition of cell proliferation was accompanied by the arrest of the cell cycle in the G2/M phase and a reduction in the expression of several regulatory marker proteins. Importantly, the _I. amara_ extract promoted apoptosis in HT-29 cells by inducing the intracellular production of reactive oxygen species. Furthermore, when administered orally at a dosage of 50 mg/kg twice a day for a duration of 4 weeks, the _I. amara_ extract significantly suppressed tumor growth in a mouse HT-29 tumor xenograft model. These results are consistent with predictions from in vitro research [29]. The primary objective of this research was to detect and quantify GSLs in various parts of the wild-growing _I. sempervirens_ plant by analyzing their desulfo counterparts through the use of UHPLC-DAD-MS/MS. Additionally, the study involved the analysis of the volatile isolates obtained from _I. sempervirens_ using different methods: hydrodistillation, CH\({}_{2}\)Cl\({}_{2}\) extraction after a 24-h autolysis period, and microwave-assisted isolation (comprising microwave distillation, microwave hydrodiffusion, and gravity). Furthermore, the antiproliferative activity of the obtained isolates was tested against two human cancer cell lines, specifically lung A549 and bladder T24, using the MTT method.