## 3 Results and Discussion  
### Physicochemical Parameter Values for Acacia Honeys  
Acacia honey in general has a low water content [14; 32; 33; 34; 35], which can be observed also in this study. The values obtained are within the limits pertaining to the honeys found in the temperate climate, especially in Europe. In order to carry out a primary interpretation of the data obtained for this parameter and to have a clear image of its variability in accordance with the geographical origin, a box plot graph of these values was constructed for each zone. The box plot diagram summarizes the charts for the five values specific to their spread in each Zone (the minimum, the first quartile, the median, the third quartile and the maximum) and the extreme values. In this chart, one can observe a central line for each box plot, representing the median of the values measured for each zone. If the median is closer to the lower margin, the value spread leans towards the left, and if it is closer to the higher margin, the spread leans towards the right. For Zone 1, the maximum of the median was found to be 16.704, for Zone 2, 17.416, and for Zone 3, the median value was 17.088. The box plot shows 50% of the values, and its size shows result variability. The horizontal lines above and below the box are traced from between the minimum and the maximum for each zone, so that the minimum values are 15.4, 15.8 and 17.1 for zones 1, 2, 3, respectively. The extreme values are placed outside the box plot and are marked with \"*\", accompanied by the sample number. In the case of water content determination, two extreme values have been found, samples S15 (belonging to Zone 1) and S25 (Zone 2) (Supplementary Figure S2). The values obtained for water activity ranged between 0.540 (S51, Zone 1) and 0.690 (S25, Zone 2) (Table 1). A water activity value of 0.562 was recorded for Slovakian acacia honey [36], 0.490 for Czech honey [33] and 0.81 for Romanian honey [37]. The L* value shows the brightness level, positive values of the parameter a* show red zone, negative values for a* show the green component, positive values for b* show the yellow Zone and negative values for b* indicate the blue component. The values for L* (Table 2) were between 50.19 and 62.70, and the average obtained after measuring all the samples was 58.96. These values are in accordance with those measured by [PERSON] et al. [38] for Slovenian honey (64.4), some Spanish honeys (51.62-56.11) [38] but are much higher than those found for Slovakian honey (11.29) [36] and other Romanian acacia honeys (48.96) [37]. The value for parameter a* (Table 2) lies between -1.28 and 5.80. The average obtained by the measuring of all the samples was \(0.08\pm 1.12\). A wide distribution can be observed when determining this parameter, as samples were situated in the green Zone (negative a*), as well as in the red Zone (positive a*). Only positive values were found for this parameter by [PERSON] et al. (4.07) [35] in the case of Slovakian honey, Romanian honey [37], whereas [PERSON] et al. [38] found only negative values for Slovenian acacia honey (-2.82), as well as [PERSON] et al. [8] for Portuguese honey. As it can be observed in Table 2, the positive and negative values obtained for this parameter cannot be linked to geographical origin or with the sample year of harvest. Each Zone has shown both negative and positive values. The same characteristic presented Spanish honey [39].