## 3 Results
The plots with inoculated grasses in crop rotation had MRA and qCO\({}_{2}\) levels 22.2% and 23.1% lower than non-inoculated plots. \begin{table}
\begin{tabular}{l c c c c} \hline \hline \multirow{2}{*}{**Treatment**} & **MRA** & **MBC** & **TOC** & **qCO\({}_{2}\)** \\ \cline{2-5}  & **mg CO\({}_{2}\) g\({}^{-1}\) dia\({}^{-1}\)** & **mg C g\({}^{-1}\)** & **g kg\({}^{-1}\)** & **Hg C-CO\({}_{2}\) g\({}^{-1}\) CBM dia\({}^{-1}\)** \\ \hline \hline \multicolumn{5}{c}{P\({}_{2}\)O\({}_{5}\) doses (kg ha\({}^{-1}\)) (P)} \\ \hline
0 & 0.71 & 0.24 & 15 & 2.9 \\
30 & 0.62 & 0.28 & 15 & 2.2 \\
60 & 0.50 & 0.30 & 15 & 1.7 \\
120 & 0.60 & 0.31 & 15 & 1.9 \\
240 & 0.75 & 0.28 & 16 & 2.7 \\ \hline \hline \multicolumn{5}{c}{Inoculation (I)} \\ \hline Without & 0.56 b & 0.28 & 15 & 2.0 b \\ With & 0.72 a & 0.28 & 15 & 2.6 a \\ \hline Test—P & 0.538 ns & 0.080 ns & 0.273 ns & 0.341 ns \\ \hline Test—I & 0.000 ** & 0.821 ns & 0.872 ns & 0.008 ** \\ \hline Test—P \(\times\) I & 0.082 ns & 0.246 ns & 0.468 ns & 0.141 ns \\ \hline CV\% & 11.39 & 10.23 & 7.71 & 16.03 \\ \hline \hline \end{tabular}  
* Means followed by different letters in the column for different treatments differed according to the LSD post-hoc test at the 5% probability level. *: significant at 1% probability by the LSD post-hoc test. ns: not significant. CV: coefficient of variation; MRA: microbial respiration activity; MBC: microbial biomass carbon; TOC: total organic carbon; qCO\({}_{2}\); metabolic quotient.  
\end{table} 
Table 21: Mean values for soil biological attributes in the 0.00–0.10 m layer, microbial respiration activity (MRA), microbial biomass carbon (MBC), total organic carbon (TOC), and metabolic quotient (qCO\({}_{2}\)) after _Brachiaria brizantha_ CV. Piata fallow in a no-tilage system, considering the residual effect of phosphate fertilization and the inoculation of preceding grasses with _A. brasilense_. Selvtria/MS, 2023/2024. Figure 11 shows soil health via [PERSON]'s model, indicating the trends for soil carbon within the treatments adopted in this study, where it can be observed that both the inoculation factor and the residual effect of P2O5 doses resulted in high and stable levels based on TOC and MRA. Still analyzing soil carbon trends in the experimental plots, the TOC and SMEA means indicated that for both the inoculation factor and the residual effect of P2O5 doses, values fell within Quadrant 1 (Q1) and Quadrant 4 (Q4), which corresponded, respectively, to healthy soil and recovering soil (Figure 12). Figure 11: Soil carbon trends (specific mean enzymatic activity (SMEA) versus total organic carbon (TOC)) in a no-tillage system, considering the residual effect of phosphate fertilization and the inoculation of preceding grasses with _A. brasilense_. Selviria/MS, 2023/2024. SMEA = ((ØG/TOC) + (AS/TOC))/2. Ø: Ø: Ø-glucosidase; AS: arylsulfatase; TOC: total organic carbon; SMEA: mean specific enzymatic activity; semt: non-inoculated area; com: inoculated area; 0, 30, 60, 120, 240: phosphorus doses in kilograms per hectare. Source: adapted from [20]. Figure 12: Soil health, based on soil carbon trends in a no-tillage system, considering the residual effect of phosphate fertilization and inoculation of preceding grasses with _A. brasilense_. Selviria/MS, 2023/2024.024. TOC: total organic carbon, SMEA: mean specific enzymatic activity; Q1: healthy soil; Q2: deteriorating soil; Q3: degraded soil; Q4: recovering soil; without: non-inoculated area; with: inoculated area; 0, 30, 60, 120, 240: phosphorus doses in kilograms per hectare. Source: adapted from [20]. The dry matter yield of _Brachiaria brizantha_ cv. Piata during fallow after harvesting the intercropped sorghum showed no interaction between the factors and no significant difference for either the residual phosphorus doses or the inoculation of preceding grain-producing grasses in rotation (Table 22).