## 3 Results
### Effect of Room Temperature Storage  
The room temperature storage experiment focused on evaluating changes in the chemical odor profile as a function of storing samples in an open or closed vial at room temperature conditions. A characterization of the SLF eggs and crickets under room temperature conditions was made over a 6-week period. Figure 8 depicts a colored odor chart highlighting the primary odor volatile array for each specimen across the 6-week period. These compounds are coined by the authors as primary odor volatiles, as they were present in all six of the replicate samples per week for each specimen type. As can be observed in the figure, the crickets had a more expansive odor profile; however, around week 3, the abundance of the open crickets, closed eggs, and closed crickets began to even. The highest relative peak area in the open eggs can be seen in week 0, while the lowest relative peak area in the open eggs can be seen in week 2. The highest relative peak area in the closed eggs can be seen in week 4, while the lowest relative peak area in the closed eggs can be seen in week 2. The highest relative peak area in the open crickets can be seen in week 0, and the lowest relative peak area in the open crickets can be seen in week 4. Finally, the highest relative peak area in the closed crickets can be seen in week 0, while the lowest relative peak area can be seen in week 5. A total of 5 primary compounds were detected in the open egg samples:  
Figure 7: PCA of the primary compounds from the SLF eggs across 6 weeks (**top**) compared to the primary compounds from the crickets across 6 weeks (**bottom**). anisole, D-limonene, 2,6-bis(1,1-dimethylethyl)-4-(1-methylpropyl)-phenol, 6,6-dimethyl-2-methylene, (1S)-bicyclo[3.1.1]heptane, and (E)-2-octenal. There was one high-frequency compound among the open eggs, D-limonene, which was detected in 5 of the 6 weeks. The low-frequency compounds were anisole and (E)-2-octenal, which were detected in only 1 of the 6 weeks. A total of 12 primary compounds were detected in the open cricket samples: 2,6-bis(1,1-dimethylethyl)-4-(1-methylpropyl)-phenol, hexanal, 1-octen-3-ol, 2-pentyl-furan, 2-butylhept-2-enol, 2-butyl-2-octenal, 5-ethyldihydro-2(3H)-furanone, octanal, 3-ethyl-2,5-dimethyl-pyrazine, nonanal, (1R)-2,6,6-Trimethylbicyclo[3.1.1]hept-2-ene, and decanal. There were two high-frequency compounds among the open crickets, 2-butylhept-2-enal and 2-butyl-2-octenal, which were detected in all six weeks of sampling. A total of seven primary compounds were detected in the closed egg samples: anisole, D-limonene, ethyl hexanoate, butanoic acid, ethyl butanoate, ethyl ester octanoic acid, and phenylethyl1 alcohol. There were two high-frequency compounds among the closed eggs: D-limonene, which was detected in five of the six weeks, and ethyl hexanoate, which was detected in four of the six weeks. A total of 14 primary compounds were detected in the closed cricket samples: hexanal, 1-octen-3-ol, 2-butylhept-2-enol, 2-butyl-2-octenal, 2-n-butyllacrolein, 6-methyl-2-heptanone, benzaldehyde, cis-4-decene, (E)-4-undecene, 2-propyl-2-heptenal, 5-ethyldihydro-2(3H)-furanone, octanal, nonanal, and heptanal. There were three high-frequency compounds among the closed crickets, 1-octen-3-ol, 2-butylhept-2-enal, and 2-butyl-2-octenal, which were detected in all six weeks of sampling. The low-frequency compounds were hexanal, 2-n-butyllacrolein, 6-methyl-2-heptanone, benzaldehyde, cis-4-decene, 5-ethyldihydro-2(3H)-furanone, and octanal, which were detected in only one out of the six weeks of sampling. Only one compound was shared between any of the open/closed eggs and the open/closed crickets, 2,6-bis(1,1-dimethyl)-4-(1-methylpropyl)-phenol, which was present in the open egg and cricket samples. This compound was detected in the open egg samples in weeks 0 and 3 and the open cricket samples in weeks 1 and 2.