# Acute and Repeated Toxicological Study of Anti-Inflammatory Herbal Formula, Yeosinsan, in Rats
## 3 Results
### Chronic oral Toxicity
#### 3.2.6 Gross Autopsy and Histopathological Examinations
In the stomach observation, in the 2000 mg\(\cdot\)kg\({}^{-1}\cdot\)d  
\begin{table}
\begin{tabular}{c c c c c} \hline
**Dosage Group (mg\(\cdot\)kg\({}^{-1}\cdot\)d\({}^{-1}\))** & **0** & **500** & **1000** & **2000** \\ \hline GUN (mg/dL) & 125.9 \(\pm\) 36.61 & 116.0 \(\pm\) 27.93 & 111.0 \(\pm\) 17.16 & 108.3 \(\pm\) 13.00 \\ BUN (mg/dL) & 16.0 \(\pm\) 1.56 & 15.8 \(\pm\) 2.62 & 15.1 \(\pm\) 1.59 & 16.9 \(\pm\) 1.56 \\ CREA (mg/dL) & 0.48 \(\pm\) 0.043 & 0.47 \(\pm\) 0.032 & 0.46 \(\pm\) 0.020 & 0.47 \(\pm\) 0.044 \\ TPg/dL) & 6.65 \(\pm\) 0.297 & 6.55 \(\pm\) 0.195 & 6.53 \(\pm\) 0.339 & 6.52 \(\pm\) 0.120 \\ ALB (g/dL) & 4.22 \(\pm\) 0.144 & 4.21 \(\pm\) 0.101 & 4.22 \(\pm\) 0.140 & 4.23 \(\pm\) 0.091 \\ A/G (ratio) & 1.74 \(\pm\) 0.102 & 1.80 \(\pm\) 0.098 & 1.83 \(\pm\) 0.105 & 1.86 \(\pm\) 0.103 \\ AST (IU/L) & 126.1 \(\pm\) 30.47 & 109.5 \(\pm\) 15.01 & 106.0 \(\pm\) 19.54 & 107.2 \(\pm\) 16.12 \\ ALT (IU/L) & 35.0 \(\pm\) 9.63 & 29.4 \(\pm\) 5.17 & 28.6 \(\pm\) 4.56 & 30.8 \(\pm\) 5.60 \\ TBIL (mg/dL) & 0.126 \(\pm\) 0.0392 & 0.113 \(\pm\) 0.0251 & 0.140 \(\pm\) 0.0193 & 0.142 \(\pm\) 0.0147 \\
**Male** & GGT (IU/L) & 1.15 \(\pm\) 1.246 & 0.43 \(\pm\) 0.250 & 0.34 \(\pm\) 0.118 & 0.31 \(\pm\) 0.181 \\ ALP (IU/L) & 268.3 \(\pm\) 57.76 & 298.8 \(\pm\) 69.89 & 258.3 \(\pm\) 57.83 & 293.5 \(\pm\) 68.38 \\ TCHO (mg/dL) & 65.7 \(\pm\) 20.54 & 57.6 \(\pm\) 21.09 & 56.1 \(\pm\) 17.60 & 49.0 \(\pm\) 9.33 \\ TG (mg/dL) & 41.7 \(\pm\) 21.23 & 27.4 \(\pm\) 14.37 & 34.2 \(\pm\) 15.89 & 24.8 \(\pm\) 12.73 \\ Ca (mg/dL) & 10.90 \(\pm\) 0.351 & 10.78 \(\pm\) 0.354 & 10.77 \(\pm\) 0.418 & 10.95 \(\pm\) 0.236 \\ IP (mg/dL) & 8.97 \(\pm\) 0.823 & 9.41 \(\pm\) 1.096 & 9.83 \(\pm\) 0.854 & 10.04 \(\pm\) 0.556 \(\ast\) \\ K (mmol/L) & 7.24 \(\pm\) 1.561 & 8.17 \(\pm\) 1.279 & 9.19 \(\pm\) 0.731 \(\ast\) & 9.16 \(\pm\) 0.819 \(\ast\) \\ CK (IU/L) & 636.7 \(\pm\) 205.21 & 594.3 \(\pm\) 132.46 & 580.4 \(\pm\) 224.39 & 557.6 \(\pm\) 0.244.43 \\ PL (mg/dL) & 100.0 \(\pm\) 18.74 & 89.8 \(\pm\) 24.06 & 90.0 \(\pm\) 18.80 & 83.0 \(\pm\) 11.18 \\ Na (mmol/L) & 142.6 \(\pm\) 1.71 & 142.4 \(\pm\) 0.97 & 142.8 \(\pm\) 0.63 & 143.8 \(\pm\) 0.63 \\ Cl (mmol/L) & 98.7 \(\pm\) 1.70 & 99.1 \(\pm\) 1.66 & 99.6 \(\pm\) 1.35 & 99.8 \(\pm\) 1.23 \\ \hline GUN (mg/dL) & 124.3 \(\pm\) 28.13 & 122.5 \(\pm\) 36.14 & 120.2 \(\pm\) 17.63 & 123.1 \(\pm\) 30.06 \\ BUN (mg/dL) & 20.1 \(\pm\) 3.43 & 16.9 \(\pm\) 2.02 & 17.7 \(\pm\) 1.54 & 18.6 \(\pm\) 3.32 \\ CREA (mg/dL) & 0.59 \(\pm\) 0.049 & 0.52 \(\pm\) 0.050 \(\ast\) & 0.52 \(\pm\) 0.037 \(\ast\) & 0.55 \(\pm\) 0.060 \\ TPg/dL) & 7.38 \(\pm\) 0.522 & 7.35 \(\pm\) 0.386 & 7.48 \(\pm\) 0.531 & 7.38 \(\pm\) 0.284 \\ ALB (g/dL) & 4.72 \(\pm\) 0.324 & 4.78 \(\pm\) 0.188 & 4.91 \(\pm\) 0.368 & 4.80 \(\pm\) 0.209 \\ A/G (ratio) & 1.77 \(\pm\) 0.067 & 1.86 \(\pm\) 0.117 & 19.1 \(\pm\) 0.101 \(\ast\) & 1.87 \(\pm\) 0.081 \\ AST (IU/L) & 115.2 \(\pm\) 36.10 & 122.3 \(\pm\) 26.10 & 98.3 \(\pm\) 12.96 & 105.5 \(\pm\) 10.66 \\ ALT (IU/L) & 38.9 \(\pm\) 29.84 & 37.8 \(\pm\) 18.01 & 29.6 \(\pm\) 8.36 & 26.8 \(\pm\) 4.48 \\ TBIL (mg/dL) & 0.133 \(\pm\) 0.0215 & 0.160 \(\pm\) 0.0204 & 0.182 \(\pm\) 0.0213 \(\ast\) & 0.194 \(\pm\) 0.0399 \(\ast\) \\ GGT (IU/L) & 0.71 \(\pm\) 0.337 & 0.93 \(\pm\) 0.645 & 0.82 \(\pm\) 0.364 & 0.87 \(\pm\) 0.315 \\ ALP (IU/L) & 170.3 \(\pm\) 62.04 & 137.4 \(\pm\) 45.26 & 169.1 \(\pm\) 40.69 & 174.3 \(\pm\) 50.54 \\ TCHO (mg/dL) & 66.3 \(\pm\) 19.06 & 60.4 \(\pm\) 15.15 & 64.8 \(\pm\) 12.15 & 63.0 \(\pm\) 14.01 \\ TG (mg/dL) & 23.5 \(\pm\) 12.20 & 18.6 \(\pm\) 6.68 & 18.1 \(\pm\) 4.18 & 15.1 \(\pm\) 6.62 \\ Ca (mg/dL) & 11.40 \(\pm\) 0.444 & 11.47 \(\pm\) 0.558 & 11.53 \(\pm\) 0.878 & 11.39 \(\pm\) 0.306 \\ IP (mg/dL) & 7.85 \(\pm\) 0.903 &dose group (G4), squamous cell hyperplasia of the forestomach was observed to be very weak (minimal) to weak (slight). These changes were observed with vesiculation or neutrophil infiltration at the limiting ridge of the forestomach and the glandular ventriculus.