## 3 Results
Position P1 demonstrated a higher initial amplitude, peaking around 200 \(\upmu\)V, whereas position P5 showed a more gradual increase, peaking at about 150 \(\upmu\)V. After this peak, the muscle activity for position P1 declined more sharply compared to position P5, which maintained a relatively steady decline. Statistical analysis revealed significant differences in muscle activity between positions P1 and P5 during two distinct phases: the early phase (0-20%) and the late phase (75-100%), as indicated by the grey-shaded regions on the statistical bar. The RBF muscle exhibited a prominent peak in EMG activity early in the cycle, between approximately 10% and 35%. Position P5 demonstrated a higher amplitude, peaking around 120 \(\upmu\)V, whereas position P1 peaked at about 60 \(\upmu\)V. Following this peak, the muscle activity for both positions declined sharply and remained low for the remainder of the cycle. However, towards the end of the cycle, a slight increase in EMG activity was observed in both positions, with position P1 showing marginally higher values. Statistical analysis revealed significant differences in muscle activity between positions P1 and P5 during the early phase (0-15%) and the late phase (55-100%) of the cycle. The RES muscle exhibited a significant peak in EMG activity around approximately 25% of the cycle. Position P5 demonstrated a higher amplitude, peaking around 70 \(\upmu\)V, whereas position P1 peaked at about 60 \(\upmu\)V. After this peak, the muscle activity for both positions declined gradually, with position P5 maintaining slightly higher values throughout the cycle than position P1. The LES muscle also showed a notable peak in EMG activity between approximately 10% and 40% of the cycle. Position P1 exhibited a higher amplitude, peaking around 120 \(\upmu\)V, while position P5 peaked at about 80 \(\upmu\)V. Following this peak, muscle activity declined for both positions, with position P1 showing consistently higher values than position P5. The analysis indicated that position P5 elicited higher muscle activity for the RES during the peak phase of the cycle compared to position P1, although these differences were not statistically significant. Conversely, for the LES, position P1 demonstrated significantly higher muscle activity during both the cycle's early and later phases than position P5. Similarly, the EMG activity profiles of the six muscles during the pedalling cycle, comparing positions P2 (green) and P5 (red), revealed distinct patterns and significant differences as indicated by the SPM [t] post-hoc analysis (Figure 5). In particular, the RTA muscle showed two distinct peaks in EMG activity. Position P5 demonstrated higher muscle activity during the first peak (0-25% of the cycle) than P2, while P2 showed higher activity during the second peak (75-100%). Significant differences were observed during the late phase of the cycle. The RVLO muscle exhibited a prominent peak early in the cycle (0-25%). Position P2 had higher muscle activity compared to P5 during this period. Significant differences were noted in the early and late phases of the cycle. The RLG muscle displayed two prominent peaks where position P2 showed higher activity during the early peak (0-25%) and the cycle's later phase (75-100%). Significant differences were observed during these phases. The RBF muscle showed a prominent peak early in the cycle (10-35%). Position P5 exhibited higher muscle activity compared to P2 during this peak. Significant differences were found in the early and late phases of the cycle. The RES muscle had a notable peak between 15% and 40% of the cycle. Position P5 demonstrated slightly higher muscle activity than P2, but no significant differences were observed during any cycle phase. Conversely, the left LES muscle peaked between 10% and 40% of the cycle. Position P2 exhibited higher muscle activity during the cycle's early and later phases than P5. Significant differences were observed during these phases.