Despite the relatively intense research employed by some research groups in the last years, the light-mediated processes are still far from being completely understood, including the spatio-temporal aspects. Moreover, most of the studies focused on the molecular and mechanical points of view and less on the biotechnological and applicative relevance of using the best light sources on _Trichoderma_ cultures. Optimizing both the production of enzymes and fungal sporulation simultaneously would benefit both industry and agriculture, but in order for this to happen it is first necessary to understand the possible influences of the various parameters involved. In this respect, it was previously reported that during conidiation of _T. reseei_, the genes codifying for the biomass-degrading enzymes were upregulated, especially in the case of cellulases and hemicellulases [30]. MAPKinases have been recently reported to be involved in light-dependent cellulase regulation with interconnections to secondary metabolism and sexual development [12]. Most studies have investigated the effects of light on _Trichoderma_ by using LED sources of light in the blue wavelength domain. To the best of our knowledge, light in the form of a laser source has been used in only one study and just for the effect of laser radiation at 355 nm on the sporulation of _Trichoderma harzianum_[31]. In that study, it was demonstrated that irradiation induced and intensified sporulation in _Trichoderma_. Both LED and lasers emit photons that produce light, but light resulting from LED sources is dispersed and multidirectional, compared to laser light, which is focused and highly localized. Therefore, the effects induced by laser could be different from the effects induced by LED. In this study, we investigated the effects of blue light generated by a 400 nm laser on the enzymatic activity and sporulation of _T. atroviride_ grown on rice husks, a sub-product of the rice production industry. We show that blue laser light can impact the protease and cellulase activity, either positively or negatively, the effect being dependent on the irradiation time and also on the incubation time in combination with the number of irradiations. There was an increase in the UV absorption of the UV-Vis spectra, which could reflect an intensification of the rice husk degradation and/or induction of fungal water-soluble metabolites. The irradiation also increased the number and size of conidia and seems to have an effect on chlamdospore formation. A positive effect on the size of conidia is reported for the first time, to the best of our knowledge. This is the first study to report the effect of light irradiation using a laser source on the cellulase and protease activities of _Trichoderma_ cultures, to the best of our knowledge. It is also the first reported study in a series of programmed investigations that aim to bring knowledge related to light effects on _Trichoderma_ to industrial and agricultural applications and develop optimized sources of irradiation in this respect. The first step in this process is to understand how irradiation parameters influence the process and how the effects evolve over time.