## 4 Conclusions  
In conclusion, we performed RNAseq to identify DEGs involved in blue light-induced fruit body development in _L. edodes_. The fruit body formation is involved in cells' morphological and molecular functions such as cell division, cell wall, and the carbohydrate metabolic process. In the phenotypic analysis, the pileus diameter grew, and the stipe length was suppressed under the blue light. The transcriptomic analysis using GSEA, KOG analysis, and KEGG pathways identified categories for the central processes of molecular biology as upregulated genes, including transcription factors in the BL condition. Among the 13 DEGs in the transcription factor category, 10 DEGs including the AT_hook, CBFD_NFYB_HMF, and HMG_box transcription factors and the fungal-specific transcription factor were upregulated in the blue light condition. CAZyme classifications of the DEGs were conducted to better understand the significant DEGs affected by the light sources on the synthesis, metabolism, and recognition of complex carbohydrates. Selected CAZyme DEGs showed six superfamilies of glycoside hydrolases (GHs) followed by four auxiliary activities (AAs), three carbohydrate esterases (CEs), and glycosyltransferases (GTs), which implies a functional change related to the cell wall polysaccharide metabolism. Furthermore, four \(\beta\)-glucosidase, one glucose oxidase, and one multicopper oxidase-related gene were upregulated in the blue light condition as DEGs for lignocellulosic genes. Interestingly, several DEGs overlapped in both the CAzyme and lignocellulosic genes. These results provide important information to further our understanding of fruit body development under blue light. The following are available online at [[https://www.mdpi.com/article/10.3390/app11156997/s1](https://www.mdpi.com/article/10.3390/app11156997/s1)]([https://www.mdpi.com/article/10.3390/app11156997/s1](https://www.mdpi.com/article/10.3390/app11156997/s1)), Figure S1: Morphological transforms according to different light cultivation conditions, Figure S2: Principal component analysis of Multi-sample BAM QC analysis by Qualimap based on specific features derived from the alignment, including coverage, GC content, insert size and mapping quality of blue (red circles) and no (blue circles) light conditions, Table S1: Summary of color difference of pileus under blue light or dark (no light)., Table S2: The list of DEG primers with 2-fold change under blue light for qRT-PCR, Table S3: The list and annotation of up and downregulated genes with 2-fold change under blue light, Table S4: The list of the differentially expressed genes enriched in GSEA analysis, Table S5: The list of the differentially expressed genes related to the carbohydrate-active enzymes (CAzyme) family, lignocellulosic, and transcription factors. Data curation, D.Y.K. ; writing--original draft preparation, D.Y.K. and J.Y.K. ; investigation, J.Y.K. ; validation, J.Y.K. ; writing--reviewing, J.Y.K. ; editing, M.-J.J. and Y.-J.P.; Conceptualization, J.Y.K. ; methodology, D.Y.K., M.-J.J. and J.Y.K. ; funding acquisition, J.Y.K. ; supervision, J.Y.K. All authors have read and agreed to the published version of the manuscript. This study was carried out with the support of the R&D Program for Forest Science Technology (Project No. 2021400C10-2125-CA02) provided by Korea Forest Service (Korea Forestry Promotion Institute). Not applicable. Informed Consent Statement: Informed consent was obtained from all subjects involved in the study. Raw sequencing data have been deposited in the NCBI Sequence Read Archive ([[https://www.ncbi.nlm.nih.gov/sra/](https://www.ncbi.nlm.nih.gov/sra/)]([https://www.ncbi.nlm.nih.gov/sra/](https://www.ncbi.nlm.nih.gov/sra/)), accessed on 19 February 2020) under the accession numbers SRR11110298, SRR11110299, SRR1110300, SRR11110301, SRR11110302, and SRR11110303. This research was supported by the National University Development Project by the Ministry of Education in 2021. The authors declare no conflict of interest. References  
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