###### Abstract  
In the current market, there is a growing interest in traditional herbal nutraceuticalticals. Therefore, herbal formulations have re-emerged as products with sought-after nutraceuticaltical and disease-preventing properties. The health-promoting effects of herbal bioactives are attributed to the active phytoconstituents of these plants. Thus, the aim of the present study was to evaluate the putative nutraceuticaltical effectiveness of the preparations of ten herbs (chamomile, purple coneflower, lemon verbena, penmyroyal, spearmint, oregano, marjoram, headed savory, sea buckthorn, and St. John's wort) by combining in silico techniques and LC-MS/MS analysis. The binding potential of the selected phenolic compounds, according to literature and web databases, was investigated by using molecular target prediction tools. Aldose reductase (AR), an enzyme of polyol pathway which is related to hypergly-induced pathologies, emerged as the most promising molecular target. The molecular docking results showed that rosmarinic acid, cafratic acid, naringenin, and quercetin presented the highest binding affinity. In a further step, the phytochemical profile of the examined infusions, obtained by LC-MS/MS analysis, revealed that the abovementioned compounds were present, mainly in the herbs of the Lamiaceae family, designating headed savory as the herbal infusion with possible significant inhibitory activity against AR. herbal infusions; in silico techniques; molecular docking; liquid chromatography-mass spectrometry (LC-MS/MS); aldose reductase (AR); phenolic compounds +
Footnote †: journal: Appl. Sci. savory (_Thymus capitatus_ [PERSON][PERSON] & [PERSON]), spearmint (_Mentha spicata_ L.), oregano (_Origanum vulgare_ ssp. hirthum), lemon verbena (_Lippi citridora_ or _Aloysia citrodora_ Palau), chamomile (_Matricaria chamomilla_ L.), purple coneflower (_Echinacea purpurea_ ([PERSON]), etc., are the most commonly used for such preparations [9] with designated nutraceutical attributes [10]. Specifically, the most characteristic aromatic plants of the Mediterranean region, such as headed savory (_Thymus capitatus_ ([PERSON]), marjoram (_Origanum majorana_ L.), oregano (_Origanum vulgare_ ssp. hirthum), pennyroyal (_Mentha pulgium_ L.), and spearmint (_Mentha spicata_ L), belong to the Lamiaceae family and are widely used in the cosmetic, food, and health industries due to their antimicrobial, antitumor, and anti-inflammatory properties [11; 12]. The species of the Asteraceae family, for instance, purple coneflower (_Echinacea purpurea_ ([PERSON]) and wild chamomile (_Matricaria chamomilla_ L.), also present significant biological activities, which are principally assigned to their phenolic content [12; 13]. The extracts of lemon verbena (_Lippiia citridora_ or _Aloysia citrodora_ Palau), a member of the Verbenaceae family, have been employed as antioxidant agents in edible coatings and nanoformulations [14], modulators of the gut microbiome [15], components for food enrichment [16], and antioxidant constituents [17]. Sea-buckthorn (_Hippophae rhamnides_ L.) of the Elaeagnaceae family has similar uses to lemon verbena. Particularly, sea-buckthorn shows therapeutic effects against respiratory and skin diseases, antibacterial and antifungal activity, and it is incorporated as a bioactive component in fortified foods [18; 19]. Moreover, St. John's word (_Hypericum perforatum_ L.), a species of the Hypericaceae family, is known for various clinical properties, such as neuroprotective, hypoglycemic, antioxidant, antimicrobial, and antidepressant activities [20; 21; 22]. Currently, the phytochemical fingerprint of herbal preparations is assessed by implementing and combining several analytical techniques, such as infrared spectroscopy (IR), liquid chromatography mass spectrometry (LC-MS), or gas chromatography (GC) with or without MS [23; 24]. Furthermore, the hyphenation of analytical methodologies with in silico tools (i.e., molecular docking) and open web servers (i.e., TargetNet, SwissTarget Prediction, MolTarPred, etc.)