## 3 Results
### Bacterial Biodiversity  
The numbers of assigned reads and alpha diversity indices of controls and treated samples are shown in Table 3. The indices show that there was not a great diversity of species and there was dominance of a low number of species. The different operational taxonomic units (OTU) found in the puree samples were grouped into 4 phyla (Figure 1), of which _Pseudomonada_ were by far the main representatives, followed by _Bacilotta_. _Actinobacteria_ and _Bacteroidota_ had relative abundances that were below 0.5% in all samples. Remarkably, samples corresponding to treatment A showed a lower relative abundance of _Pseudomonada_ and a higher relative abundance of _Bacilotta_. Figure 1: Bacterial diversity of puree samples at phylum level. C, untreated controls. P, puree inoculated with _Paenibacillus dendritiformis_ UJA2219. E, puree treated with partially-purified extract(1.5 M eluate). A, pure treated with partially-purified extract (60% acetonitrile). After 24 h incubation, total bacterial DNA was extracted from the purees and the 16S rDNA gene V3-V4 region was amplified and sequenced using Illumina technology. The DNA sequence reads obtained were assigned to operational taxonomic units (OTUs). OTUs were grouped by taxonomic level. The mean values of sample replicates are shown. These phyla included representatives of 26 families (Figure 2). Of them, 8 families had relative abundances of at least 2% in at least one of the samples (_Pseudomonasaceae_, _Enterobacteriaceae_, _Moraxellaceae_, _Bacillusaceae_, _Enterococcaceae_, _Streptococcaceae_, _Xanthomonadaceae_ and _Leuconostaceae_). _Pseudomonasaceae_ and _Enterobacteriaceae_ were the families with higher relative abundances. The relative abundance of _Pseudomonasaceae_ was highest in sample E and lowest in sample A. Family _Moraxellaceae_ had the lowest relative abundance in sample E. _Xanthomonadaceae_ only had a relative abundance > 2.0% in sample E. The following families belonging to the phylum _Bacillusota_ had the highest relative abundances in sample A and either low or very low relative abundances in the other samples: _Bacillusaceae_, _Enterococcaceae_ and _Streptococcaceae_. Figure 2: Bacterial diversity of puree samples at family level. C, untreated controls. P, puree inoculated with _Paenibacillus dendritiformis_ UJA2219. E, puree treated with partially-purified extract (1.5 M eluate). A, puree treated with partially-purified extract (60% acetonitrile). After 24 h incubation, total bacterial DNA was extracted from the purees and the 16S rDNA gene V3-V4 region was amplified and sequenced using Illumina technology. The DNA sequence reads obtained were assigned to operational taxonomic units (OTUs). OTUs were grouped by taxonomic level. The mean values of sample replicates are shown. At the genus level, a total of 37 genera were detected (Figure 3). Of them, 9 had relative abundances \(\geq\) 2% in at least one sample (_Pseudomonas_, _Acinetobacter_, _Enterobacter_, _Klebsiella_, _Stenotrophomonas_, _Bacillus_, _Enterococcus_, _Lactococcus_ and _Leuconostoc_). Most of the OTUs corresponding to family _Enterobacteriaceae_ could not be assigned at the genus level. Among the assigned OTUs, genus _Enterobacter_ was the main representative, followed by _Klebsiella_. _Escherichia-Shigella_ had very low relative abundances. _Pseudomonasaceae_ was represented mainly by genus _Pseudomonas_. A global representation of the results of bacterial diversity showing the main taxonomic levels of the controls and treated samples is shown in Figure 4.  
Figure 3: Bacterial diversity of pure samples at genus level. C, untreated controls. P, puree inoculated with _Paenibacillus dendritiformis_ UJA2219. E, puree treated with partially-purified extract (1.5 M eluate). A, puree treated with partially-purified extract (60% acetonitrile). After 24 h incubation, total bacterial DNA was extracted from the purees and the 16s rDNA gene V3–V4 region was amplified and sequenced using Illumina technology.