# Novel Adamantane Derivatives: Synthesis, Cytotoxicity and Antimicrobial Properties
## 3 Results
### Cytotoxicity  
The 24 and 48 h cultures of L929 cells with the compounds marked **7**, **10** and **21** showed that the most cytotoxic compounds were **10** at a dose of 100 \(\mu\)M during 24 h and 48 h of culture and **21**--at a dose of 100 \(\mu\)M during 48 h of culture. However, none of the tested compounds caused a cytotoxic effect below 50% (Tables 2 and 3, Figures S1-S6 in Supplementary Materials). The 48 h culture of A549 with the tested compounds (**7**, **10**, **21**) showed that the best effect in promoting cytotoxicity was obtained with the use of compound **10** at a concentration of 25 and 150 \(\mu\)M and derivative **21** at a concentration of 100 \(\mu\)M but with no compound could be given the IC\({}_{50}\). On the other hand, during the 24 h culture of the A549 cell line, these compounds caused a slight increase in cell proliferation (Tables 4 and 5, Figures S7-S12 in Supplementary Materials). The 24 h culture of T47D cells with the examined compounds (**7**, **10**, **21**) showed that the main antiproliferative effect was observed with compounds **7** at a dose of 25-50 \(\mu\)M and **21** at a dose of 25 \(\mu\)M. During the 48 h cell culture, only compound **21** at a dose of 25 \(\mu\)M caused such an effect. However, none of the tested compounds caused a cytotoxic effect below 50% (Tables 6 and 7, Figures S13-S18 in Supplementary Materials). \begin{table}
\begin{tabular}{c c c c c c c c c c c c c} \hline \hline  & & \multicolumn{4}{c}{**7**} & \multicolumn{4}{c}{**10**} & \multicolumn{4}{c}{**21**} \\ \cline{2-13}  & & \multicolumn{4}{c}{**Repeat**:} & \multicolumn{4}{c}{**Repeat**} & \multicolumn{4}{c}{**Repeat**} \\ \cline{2-13}
**Compound No/Concentration** & \multicolumn{4}{c}{**No**} & \multicolumn{4}{c}{**No**} & \multicolumn{4}{c}{**No**} & \multicolumn{4}{c}{**No**} & \multicolumn{4}{c}{**No**} & \multicolumn{4}{c}{**No**} & \multicolumn{4}{c}{**No**} & \multicolumn{4}{c}{**No**} & \multicolumn{4}{c}{**No**} \\ \cline{2-13}
**(\(\mu\)M)** & **1** & **2** & **3** & **4** & **1** & **2** & **3** & **4** & **1** & **2** & **3** & **4** \\ \hline
**control** & 91 & 104 & 89 & 95 & 87 & 112 & 103 & 96 & 101 & 94 & 96 & 103 \\ \hline
**5** & 96 & 104 & 101 & 97 & 100 & 106 & 118 & 109 & 98 & 96 & 109 & 104 \\ \hline
**10** & 94 & 89 & 96 & 92 & 95 & 81 & 103 & 107 & 96 & 91 & 93 & 111 \\ \hline
**25** & 97 & 97 & 78 & 61 & 74 & 72 & 64 & 69 & 87 & 96 & 94 & 85 \\ \hline
**50** & 66 & 64 & 55 & 63 & 79 & 86 & 78 & 89 & 59 & 85 & 53 & 51 \\ \hline
**100** & 76 & 87 & 66 & 68 & 54 & 47 & 59 & 62 & 79 & 84 & 85 & 91 \\ \hline
**150** & 81 & 84 & 98 & 91 & 88 & 79 & 94 & 92 & 86 & 88 & 93 & 99 \\ \hline
**200** & 76 & 79 & 98 & 89 & 85 & 73 & 77 & 69 & 80 & 97 & 99 & 101 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: The cell proliferation in % after 24 h exposition on studied compounds in L929 cell line. \begin{table}
\begin{tabular}{c c c c c c c c c c c c} \hline \hline  & & \multicolumn{4}{c}{**7**} & \multicolumn{4}{c}{**10**} & \multicolumn{4}{c}{**21**} \\ \cline{2-13}
**Compound No/Concentration** & \multicolumn{4}{c}{**Repeat**:} & \multicolumn{4}{c}{**Repeat**} & \multicolumn{4}{c}{**Repeat**} \\ \cline{2-13}
**(\(\mu\)M)** & **No** & **No** & **No** & **No** & **No** & **No** & **No** & **No** & **No** & **No** & **No** \\ \cline{2-13}
**control** & 97 & 101 & 108 & 102 & 98 & 95 & 97 & 101 & 109 & 98 & 105 & 100 \\ \hline
**5** & 113 & 100 & 98 & 97 & 93 & 116 & 1 During the 24 and 48 h of cultures of the HeLa cell line, it was shown that all tested compounds did not significantly inhibit cell proliferation. The only exception was compound **10** at a dose of 25 \(\upmu\)M during 48 h culture, which resulted in a decrease in proliferation of approximately 60% (Tables 8 and 9, Figures S19-S24 in Supplementary Materials).