# In Vivo Biosynthesis of Au and Ag NPs Using Two _Medicago sativa_ L. Genotypes
## 3 Results
### Obtaining Plant Suspension from Seedings for Spectrophotometry
However, the results changed unevenly, as only a part of the results (7 of 24) showed statistically significant changes. Table 6 shows the average data of the three measurements of light absorption for the control group and the experimental groups of seedlings of both genotypes after 48 h of exposure to precursors. These results showed that the changes in light absorption did not fluctuate as often as was seen in the results after 24 h of precursor exposure in alfalfa. In samples of genotype _'Kometa'_ that received HAuCl4 acid for 48 h, light absorption was increased statistically significantly compared to the control in samples where absorption was measured at a wavelength of 650 nm. However, samples where absorption was measured at 450 nm showed statistically insignificant light absorption increase compared to the control. An increase in absorbance was observed at both wavelengths of light (450 and 650 nm). In samples of the _'Kometa'_ genotype that received AgNO3 nitrate for 48 h and had absorption measured at 470 nm, light absorption was increased compared to the control group at all concentrations (50, 100, and 200 mg/L). However, this increase was not statistically significant. At 350 nm, its absorption in the _'Kometa'_ samples also increased statistically insignificantly. In _'la Bella'_ genotype samples that received HAuCl4 acid for 48 h, light absorption increased statistically significantly at all acid concentrations (50, 100, and 200 mg/L) compared to the control group. For these samples, absorption was measured at a wavelength of 450 nm. However, samples treated at a wavelength of 650 nm and an acid concentration of 50 mg/L showed a decrease in absorbance compared to the control, but this was not statistically significant. Samples with the same light wavelength but a higher acid concentration (100 and 200 mg/L) showed a statistically significant decrease in light absorption compared to the control group. The consistency of the results between samples within the same experimental group was also demonstrated by the remaining samples with AgNO3 chloride; however, these results did not rule out differences between the experimental groups. The results with AgNO3 showed that light absorption decreased statistically significantly at almost all acid concentrations (250 and 1000 mg/L) compared to the control group when absorption of the samples at 470 nm was measured. However, light absorption decreased statistically insignificantly at samples with an acid concentration of 500 mg/L. Results when a wavelength of 350 nm was used showed that light absorption at all acid concentrations (250, 500, and 1000 mg/L) increased statistically insignificantly compared to the control group. These results are shown in Table 6.  
In 10 of 24 samples, the results from light absorption data at different wavelengths after 48 h of exposure to precursors showed statistically significant changed light absorbance values. This indicates that 48 h of exposure to the precursor caused changes in light absorption more often. Results show that HAuCl4 increased light absorption in _'Kometa'_ samples statistically significantly when absorption was measured at 450 nm. While in _'la Bella'_ samples, light absorption was statistically significantly decreased when AgNO3 at concentrations of 250 mg/L and 1000 mg/L were used and when absorption was measured at 470 nm. Absorption also statistically significantly decreased when HAuCl4 was used at concentrations of 100 mg/L and 200 mg/L and absorption was measured at 650 nm.