# Evaluation of Yukmiijihwang-Tang as a Novel Preventive Agent in Ovalbumin-Induced Murine Asthma
## 3 Results
### Toxicity Analysis of YJT  
The cytotoxic effect of various concentrations of YJT (7.81 to 500 \(\upmu\)g/mL) was examined in NCI-H292 cells. As shown in Figure 2, YJT had no cytotoxicity at any of the concentrations tested.  
\begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline \multirow{2}{*}{**No.**} & \multirow{2}{*}{**RT (min)**} & \multicolumn{3}{c}{**Precursor Ion (m/z)**} & \multirow{2}{*}{**Error**} & \multirow{2}{*}{**Formula**} & \multirow{2}{*}{**MS/MS Fragments (m/z)**} & \multirow{2}{*}{**Identifications**} \\ \cline{2-2} \cline{5-8}  & & **Calculated** & **Estimated** & **Adduct** & & & & & \\ \hline
1 & 4.62 & 373.1140 & 373.1140 & M-H & 2.8408 & C\({}_{16}\)H\({}_{22}\)O\({}_{10}\) & - & Geniposidic acid [21] \\ \hline
2 & 4.89 & 375.1297 & 375.1295 & M-H & 2.5146 & C\({}_{16}\)H\({}_{22}\)O\({}_{10}\) & 375.1292, 213.0761, 169.0858, 151.0752 & \multirow{2}{*}{Loganic acid [22]} \\ \hline
3 & 5.12 & 451.1457 & 451.1457 & M+HCO\({}_{2}\) & 2.3800 & C\({}_{17}\)H\({}_{20}\)O\({}_{11}\) & 243.0871, 179.0547, 155.0338, 141.0544, 1253.0439, 59.0122 & \multirow{2}{*}{Morroniside [22]} \\ \hline
4 & 5.63 & 505.1557 & 505.1564 & M+HCO\({}_{2}\) & 2.4165 & C\({}_{29}\)H\({}_{28}\)O\({}_{12}\) & 293.0883, 165.0546 & \multirow{2}{*}{Paeeonolide [23]} \\ \hline
5 & 5.68 & 435.1508 & 435.1506 & M+HCO\({}_{2}\) & 2.1421 & C\({}_{17}\)H\({}_{26}\)O\({}_{10}\) & 227.0917, 127.0388, 101.0230 & \multirow{2}{*}{Loganin [22]} \\ \hline
6 & 5.91 & 403.1246 & 403.1245 & M+HCO\({}_{2}\) & 2.6154 & C\({}_{16}\)H\({}_{22}\)O\({}_{9}\) & 195.0651, 125.0229, 81.0330 & \multirow{2}{*}{Sweeroside [22]} \\ \hline
7 & 6.37 & 525.1614 & 525.1610 & M+HCO\({}_{2}\) & 1.4157 & C\({}_{23}\)H\({}_{28}\)O\({}_{11}\) & 449.1473, 327.1082, 121.0280 & \multirow{2}{*}{Paeoniflorin [24]} \\ \hline
8 & 6.78 & 463.0882 & 463.0882 & M-H & 2.3612 & C\({}_{21}\)H\({}_{29}\)O\({}_{12}\) & - & Isoquecitrin [25], & \\ \hline
9 & 7.38 & 623.1981 & 623.1981 & M-H & 1.6246 & C\({}_{29}\)H\({}_{36}\)O\({}_{10}\) & 623.1980, 461.1662, 161.0232 & \multirow{2}{*}{Isoacteoside [26]} \\ \hline
10 & 8.86 & 599.1770 & 599.1769 & M-H & 1.7083 & C\({}_{36}\)H\({}_{32}\)O\({}_{13}\) & 599.1754, 447.1300, 137.0231, 121.0280 & \multirow{2}{*}{Mudaniposide C [23]} \\ \hline
11 & 9.55 & 301.0354 & 301.0352 & M-H & 3.1166 & C\({}_{15}\)H\({}_{10}\)O\({}_{7}\) & 301.0350, 178.9976, 151.0022 & \multirow{2}{*}{Quercetin [27]} \\ \hline
12 & 10.89 & 629.1876 & 629.1875 & M+HCO\({}_{2}\) & 1.6180 & C\({}_{39}\)H\({}_{32}\)O\({}_{12}\) & 431.1339, 165.0545 & \multirow{2}{*}{Benzoylpaeoniflorin [23]} \\ \hline \end{tabular}
\end{table}
Table 1: Phytochemicals identified from YJT by UPLC-DAD-MS/MS.  
Figure 1: UPLC-DAD-MS/MS analysis of YJT: the ultraviolet (UV) (254 nm) base peak chromatogram in negative ion mode and the extracted ion chromatogram (EIC) of the identified phytochemicals in YJT. In the EIC, the peaks are labeled as follows: 1: geniposidic acid, 2: loganic acid, 3: morroniside, 4: paeonolide, 5: loganin, 6: sweroside, 7: paeoniflorin, 8: isoquercirtin/hyeroside, 9: isoatecoside, 10: mudaniposide C, 11: quercetin, and 12: benzoylpaeoniflorin.