## 3 Results
Voltage waveforms (**a**) and current waveforms (**d**) during operation of the DBD plasma in the condition at 760 Torr, voltage waveforms (**b**) and current waveforms (**e**) during the operation of the corona discharge plasma in the condition at 760 Torr, and voltage waveforms (**c**) and current waveforms (**f**) during the operation of the corona discharge plasma in the condition at 10 Torr were measured using an oscilloscope with a high-voltage probe. anti-PrP antibody (SAF83) was performed. In the control sample, PrPres was detected after the 1 st round of PMCA followed by digestion with PK (PK(+)), but no such band was detected in the RENO-S130-treated samples (non-lumen or Eco mode). Likewise, PrPres was also found in control samples that had been analyzed in a 2 nd round of PMCA, prior to digestion with PK (PK(+)). However, PrPres was not found in RENO-S130-treated samples (non-lumen or Eco mode) after the 2 nd round of PMCA in PK(+).Total PrP, comprising mainly PrP\({}^{\text{C}}\) substrate, was present in all PK(-) samples at similar intensities following the 1 st and 2 nd rounds of PMCA. Taken together, these results show that the in vitro proliferation activity of the prion was decreased by both types of RENO-S130 treatment (non-lumen or Eco mode). These observations suggest that the two modes of RENO-S130 treatment eliminate PrP\({}^{\text{SC}}\) amplification capability. Next, Chandler prion-infected mouse brain homogenate, either treated with the RENO-S130 device (non-lumen or Eco mode) or untreated (Control), were intracerebrally injected into C57 BL/6] mice and their behavior and survival monitored over time (Figure 5). All six mice in the Control group showed unusual behavior, including tremors and ataxia, and died before 227 days ([PERSON] et al., 2018). Of the six mice in each of the two RENO-S130 treatment groups (non-lumen and Eco mode), five showed no clinical symptoms of prion disease up to the end of the study period (576 days). One mouse in the non-lumen group died on day 401 and one mouse in the Eco group died on day 181, although neither displayed abnormal behavior during the study period. A log-rank test demonstrated that the survival curve was significantly different between the Control group and the non-lumen group or Eco group. No significant differences were observed between the non-lumen group and Eco group. Figure 4: Protein misfolding cyclic amplification (PMCA) was used to assess the effectiveness of inactivation of scrapie prion after treatment with the RENO-S130 device. Uninfected mouse brain homogenate was used as PrP\({}^{\text{C}}\) substrate. Untreated control (C) or RENO-S130-treated (nonL and Eco) samples retrieved from the spots on the cover glasses were diluted 1:10 with PrP\({}^{\text{C}}\) substrate and analyzed in a 1 st round of PMCA. Thereafter, a 2 nd round was performed following an additional 1:10 dilution with PrP\({}^{\text{C}}\) substrate. An aliquot taken from the various samples was then incubated with or without PK (20 \(\upmu\)g/mL) at 37 \({}^{\circ}\)C for 60 min to reveal PrPres (**a**) and total PrP (**b**), respectively. To detect PrPres and total PrP, samples were subject to Western blot analysis with SAF83, an anti-PrP antibody. Size markers (kDa) are shown on the left-hand side of the blots. The incubation time of the disease mirrored survival, with a marked dissimilarity between the Control group and the non-lumen group or Eco group. The mean incubation time of mice injected with untreated prion was 191.0 \(\pm\) 9.0 days [30]. By contrast, one mouse in the non-lumen group died on day 401, while 5 mice survived more than 576 days. Likewise, one mouse in the Eco group died on day 181, while 5 mice survived more than 576 days. Overall, these findings suggest that the incubation time of mice injected with RENO-S130 treated (non-lumen or Eco mode) prion-infected brain tissue was longer than for the Control group.