## 3 Results
### Macro- and Micronutrient Status in the Soybean Grain
#### 3.1.2 Macro- and Microelement Accumulation in the Grain  
On average for both years in this study, the Zn and Se biofortification treatment showed different influences on macro- and microelements content in soybean grain (Figure 1a-g).  
For macronutrient status, the biofortification treatments showed significant influence only in the case of accumulated P in the soybean grain (Figure 1a-d). The highest content of P in this study was determined in the control treatment (6.89 g/kg), whereas biofortification treatments decreased the content of P (Figure 1a). In regard to varieties, the Toma variety had the highest accumulation of P (7.19 g/kg), and the Sonja variety accumulated less P (6.22 g/kg). The biofortification treatments nor variety did not have a significant influence on the average K content in the soybean grain, which was 20.06 g/kg (Figure 1b). For the Ca content, there were no significant differences among the biofortification treatments, whereas for the varieties, the differences among the mean accumulated Ca were significant (Figure 1c). The Ca content varied from 2.00 g/kg for the Ika variety to 2.82 g/kg for the Toma variety. The Mg content in the soybean grain was, on average, for all treatments, 2.54 g/kg (Figure 1d). Here, the varieties showed significant differences in relation to the accumulation of Mg, so the Sunc variety accumulated the highest Mg content (2.78 g/kg) and the Sonja variety accumulated the lowest amount of Mg (2.41 g/kg).  
\begin{table}
\begin{tabular}{c c c c c c c c c c} \hline \hline
**Source of** & **P** & **K** & **Ca** & **Mg** & **Fe** & **Mn** & **Cu** & **Zn** & **Se** \\ \cline{2-10}
**Variation** & & & & & **mg kg\({}^{-1}\)** & & & & & \(\mu\) **kg\({}^{-1}\)** \\ \hline Y & \textless{}0.0001 & \textless{}0.0001 & \textless{}0.0001 & ns & \textless{}0.0001 & \textless{}0.0001 & \textless{}0.0001 & \textless{}0.0001 & \textless{}0.0001 \\ V & \textless{}0.0001 & ns & \textless{}0.0001 & \textless{}0.0001 & \textless{}0.0001 & \textless{}0.0001 & ns & \textless{}0.0001 & \textless{}0.0001 \\ B & \textless{}0.0001 & ns & ns & ns & * & \textless{}0.0001 & ns & \textless{}0.0001 & \textless{}0.0001 \\ Y \(\times\) V & \textless{}0.0001 & \textless{}0.0001 & \textless{}0.0001 & ** & * & \textless{}0.0001 & \textless{}0.0001 & \textless{}0.0001 & ns \\ V \(\times\) B & ns & ns & \textless{}0.0001 & ns & ns & \textless{}0.0001 & ns & \textless{}0.0001 & \textless{}0.0001 \\ Y \(\times\) B & * & ns & ns & ns & ns & \textless{}0.0001 & ** & \textless{}0.0001 & \textless{}0.0001 \\ Y \(\times\) V \(\times\) B & ns & ns & ns & ns & \textless{}0.0001 & ** & \textless{}0.0001 & \textless{}0.0001 \\ \hline \hline \end{tabular}  
* ns: non-significant;
* significant at \(p<0.05\); *
* significant at \(p<0.01\).  
\end{table}
Table 2: Analysis of variance (ANOVA) for the year (Y), soybean variety (V), and Zn and Se biofortification treatments (B) for macro- and micronutrients in the soybean grain.  
content of Fe in the grain was determined at the Se_3 Zn_2 treatment, which was 12.5% lower than the control. Among the varieties, the Fe content varied from 65.0 mg/kg (Lucija) to 82.3 mg/kg (Toma). Biofortification significantly influenced the Mn status in the soybean grain (Figure 1f), which was with Se foliar application on average 29.24 mg/kg and with Zn and simultaneous Se and Zn application on average 32.31 mg/kg. Soybean varieties in this study showed significant differences in Mn accumulation, which was the highest for the Lucija variety (33.07 mg/kg) and the lowest for the Ika variety (25.96 mg/kg). The Cu content in the soybean grain was on average 17.66 mg/kg, and it was not significantly affected by biofortification or variety (Figure 1g).