## 3 Results
### FT-ICR MS Analysis  
FT-ICR MS analysis applied to hydroalcoholic and organic Bligh-Dyer extracts allowed us to simultaneously detect many compounds based on their accurate mass and specific isotope pattern. Different metabolomic databases enabled the identification of more than \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Metabolite** & **Assignment** & **1H (ppm)** & \begin{tabular}{c} **Multiplicity** \\ **[J(Hz)]** \\ \end{tabular} & \
\begin{tabular}{c} **13C** \\ **(ppm)** \\ \end{tabular} &
\begin{tabular}{c} **mg/100 g of** \\ **Sample** \\ \end{tabular} \\ \hline \multirow{3}{*}{Phenylalanine} & CH-2,6 ring & 7.32 & m & 130.5 & 167.43 \(\pm\) 5.08 \\  & CH-3,5 ring & 7.42 * & m & 130.1 & \\  & CH-4 ring & 7.37 & m & 128.7 & \\  & \(\alpha\)-CH & 4.15 & & & 977.76 \(\pm\) 17.27 \\  & \(\beta\), \(\beta\)’-CH\({}_{2}\) & 2.06; 2.35 & m & & \\  & \(\gamma\)-CH\({}_{2}\) & 2.01 * & m & 25.3 & \\  & \(\delta\), \(\delta\)’-CH\({}_{2}\) & 3.35; 3.43 & m & & \\  & \(\delta\)-CH\({}_{2}\) & 3.28 & t [6.5] & 48.4 & 259.83 \(\pm\) 13.73 \\  & N-CH\({}_{2}\) & 3.44 * & t [6.5] & 36.3 & \\  & \(\alpha\)-CH & 3.60 & & 61.4 & 88.89 \(\pm\) 6.86 \\  & \(\beta\)-CH & 4.27 & & 67.1 & \\  & \(\gamma\)-CH\({}_{3}\) & 1.34 * & d [6.6] & 21.2 & \\  & CH-4, ring & 7.72 & d [8.1] & & 73.10 \(\pm\) 2.54 \\  & CH-5, ring & 7.20 & & & \\  & CH-6, ring & 7.27 & & & \\  & CH-7, ring & 7.51 * & d [8.1] & & \\  & CH-2,6 ring & 6.89 * & d [8.6] & 116.9 & 537.32 \(\pm\) 4.82 \\  & \(\gamma\)-CH\({}_{3}\) & 7.20 & d [8.6] & 129.5 & \\  & C-4 ring & & & 155.5 & \\  & \(\alpha\)-CH & 3.63 & & 61.6 & 390.18 \(\pm\) 17.35 \\  & \(\beta\)-CH & 2.30 & & 30.3 & \\  & \(\gamma\)’-CH\({}_{3}\) & 0.99 & d [7.06] & 18.0 & \\  & \(\gamma\)’-CH\({}_{3}\) & 1.05 & d [7.06] & 19.1 & \\
**Organic** & & & & & \\
**acids** & & & & & \\  & COO’ & & & 182.5 & 359.64 \(\pm\) 16.25 \\  & \(\alpha\)-CH\({}_{3}\) & 1.92 * & s & 24.4 & \\  & & & & & \\  & \(\beta\)-CH\({}_{2}\) & 2.41 * & s & 35.1 & \\
**Other metabolites** & & & & & \\  & \(\alpha\)-CH\({}_{2}\) & & & 68.9 & \\  & & & & \\  & CH-1,3 & 3.56 * & dd [11.7; 6.5] & 63.6 & 1175.31 \(\pm\) 23.08 \\ Glycerol & CH-2,-2 & 3.79 & m & 55.4 & \\  & CH-1/3/\({}^{\prime}\) & 3.66 & dd [11.7; 4.3] & 63.6 & \\ \hline \hline \end{tabular}
\end{table} 
Table 1: _Cont._500 molecular formulas, as shown in Table S1, spread between hydroalcoholic and organic Bligh-Dyer extracts. Among these, about 350 molecular formulas were attributed using the free tool MassTRIX [22]. Similarities and differences between the hydroalcoholic and organic extracts are underlined in a two-way Venn diagram; around 39% of the molecular formulas were revealed to be common to both hydroalcoholic and organic extracts, as shown in Figure 2.  
Considering the high number of molecular formulas, van Krevelen diagrams (vKds) were built to provide a visual distribution of the main identified molecular classes [23]. In particular, elemental formulas from each sample were inserted into the diagrams, where the molar hydrogen to carbon ratio (H/C) is plotted against the molar ratio of oxygen to carbon (O/C), thus affording an overview of the molecular families. The hydroalcoholic extract presented a high density of metabolites in the region of lipids, terpenoids, and polykefides, followed by amino acids, whereas the organic portion displays a higher number of entries in the lipid and terpenoid areas, as shown in Figure 3A,B. Along the trend lines in the corresponding diagrams in Figure 3C,D, it is possible to identify structural relationships due to chemical reactions among groups of compounds. For instance, hits along vertical green A lines, associated with (de)hydrogenation processes, comprise decenedioic/sebaci acids (C\({}_{10}\)H\({}_{16}\)O\({}_{4}\)/C\({}_{10}\)H\({}_{18}\)O\({}_{4}\)), linoleicamide/oleamide/stereamide (C\({}_{18}\)H\({}_{33}\)NO/C\({}_{18}\)H\({}_{35}\)NO/C\({}_{18}\)H\({}_{37}\)NO), and hydroxy-octadecanoylcarnitine (C\({}_{25}\)H\({}_{47}\)NO\({}_{5}\)/C\({}_{25}\)H\({}_{49}\)NO\({}_{5}\)).