## 3 Results and Discussion
### Transcriptome Sequencing and Functional Annotation of Differentially Expressed Genes (DEGs)
In addition, pileus differentiation and development require light stimulation, and different light qualities can produce different morphological features [27]. In this study, genes related to signal transduction, transcription/post-translation, and cell division, including DNA replication and repair, were upregulated in the blue light condition. The results indicate that blue light affected the growth and development at the cellular level and caused different morphogenesis compared with the no-light condition. CAZyme Encoding Genes under Different Light Sources Mapped to the Differentially Expressed Genes (DEGs)  
We analyzed families of structurally related catalytic and carbohydrate-binding modules of enzymes that degrade, modify, or create glycosidic bonds using CAZy in DEGs (Figure 4). A total of 16 and 10 genes related to the carbohydrate-binding modules of enzymes were identified as up and downregulated DEGs, respectively. In the upregulated genes, the highest distribution of DEGs belonged to superfamilies with six glycoside hydrolases (GHs), followed by four auxiliary activities (AAs), three carbohydrate esterases (CEs), and glycosyltransferase (GTs). Among the CAZyme genes, GHs mainly hydrolyze glycosidic bonds between two or more sugars or a sugar and non-sugar moiety within carbohydrates or oligosaccharides and play important roles in the developmental process, such as cell wall modification, cellulosic biomass metabolism, energy metabolism, and stress resistance [28; 29; 30; 31; 32]. In this study, the GH1 (1 gene), GH3 (3 genes) GH17 (1 gene), and GH71 (1 gene) families were upregulated under the blue light condition (Figure 4A), and the ratio of the pileus diameter to the stipe length had the highest value under the blue light condition and the lowest value under the dark condition (Figure S1, Table 1). In previous reports, the GH1 family  
Figure 4: Heat map analysis and hierarchical clustering of up and downregulated genes under blue light and no-light conditions for (**A**) CAZyme, (**B**) lignocellulosic, and (**C**) transcription factor. Each column represents an experimental mushroom sample under a different light source (no light and blue light), and each row represents an identified gene. Yellow and blue indicate the upregulation and downregulation of each gene, respectively. Expression values of genes were transformed into Z-score values. was found to participate in a number of developmental processes and stress responses, including cell wall modification, plant hormone activation or deactivation, and herbivore resistance [29]. GH3 enzymes carry out a range of functions, including cellulosic biomass degradation, plant and bacterial cell wall remodeling, energy metabolism, and pathogen defense [30; 31], and the family GH17 mediates diverse processes that include growth, callose deposition, and pathogen defense [32]. Therefore, it is suggested that upregulated GH families under blue light could positively affect the pileus diameter. In addition, AA1 (1 gene), AA2 (1 gene), AA3 (1 gene), and AA14 (1 gene) of the AA family, and GT1 (1 gene), GT15 (1 gene), and GT22 (1 gene) of the GT family were upregulated under the blue light condition (Figure 4A). Auxiliary activity (AA) classification consists of the potential ability to help the original GH, PL, and CE enzymes gain access the carbohydrates encrusted in the plant cell wall [33]. In addition, glycosyltransferases (GTs) influence growth, development, cell division, and environmental responses [34]. Therefore, we can suggest that the upregulated CAzyme gene family, such as GHs, AAs, and GTs under blue light condition, are positively related to pileus development and differently affect the ratio of pileus diameter to the stipe length. Using the lignocellulosic genes of _L. edodes_ annotated by [PERSON] et al.