## 4 Discussion
### RGR, Statistical Analysis and Dose Response  
The results clearly show that for the control, growth was not impaired at all, as \(W_{2}>W_{1}\). However, the reverse is the case for plants treated with metronidazoleze solution. For these plants, growth was hampered, as \(W_{2}<W_{1}\), the extent of retardation being proportional to the antibiotic concentration applied [43]. The values obtained further buttress the impact of metronidazoleze on the growth of soybean plant (_Glycine max_). The treated plants widened drastically. Additionally, the mean RGR values for the control plants indicated an increase in weight in the dry biomass of the replicates from \(t_{1}\) to \(t_{2}\); however, mean RGR values for the antibiotic-treated plants were all negative-the higher concentration, i.e., 100 mg/L giving higher negative values than the 50 mg/L treatment. This shows that plants that took in most, showed the greatest wilting [43]. Also, the growth of soybean plants (_Glycine max_) was affected by increasing concentrations of metronidazoleze [29]. A few previous studies investigated poisoning of leguminous plants, including soybean (_Glycine max_) by different antibiotics. In some cases, the antibiotic killed the plants, while in others they only retarded plant growth [29; 44; 45]. In the present study, the phytotoxicity of high concentrations of metronidazoleze to exposed soybean (_Glycine max_) was explored. Toxicological effects of metronidazoleze increased with increasing concentration--relative growth rate decreased with increasing metronidazoleze concentration, in the order: RGR control \(>\) RGR 50 mg/L \(>\) RGR 100 mg/L (Figure 1). [PERSON] [44; 45] applied high concentration (160 mg/L) of animal growth-promoting antibiotics, chlortetracycline and oxytetracycline to corn (_Zea mays_ L. var. Grand Valley hybrid SX 121), pinto bean (_Phaseolus vulgaris_ L. var. University of Idaho 114), radishes (_Raphanus sativus_ L. var. White Icicle), and wheat (_Triticum aestivum_ L. var. Fielder). While the legume, pinto bean (_Phaseolus vulgaris_) was affected by the exposure, the three other crops were unaffected. Additionally, whereas pinto bean (_Phaseolus vulgaris_) planted in sandy loam soil polluted with either antibiotic in separate replicates were poisoned, those planted in clay soil polluted with the same antibiotics showed no ill effects. In contrast to the unexposed group, pinto bean plants (_Phaseolus vulgaris_) grown in sandy loam absorbed smaller quantities of plant nutrients from the soil and sustained less nitrogen fixation; they were smaller, weighed less, and throtified smaller bean yields. A plausible explanation of this observation could be that the drug molecules adsorbed to the clay soil, and so pinto bean plants (_Phaseolus vulgaris_) were inhibited from absorbing the antibiotics from the soil [44; 45]. He concluded that the extent of antibiotic toxicity to plant growth is strongly influenced by plant sensitivity to the contaminant drug, and thesoil typical features [45]. Research suggests that environmental fate and transport of antibiotics is influenced by a number of factors: physical properties of the compound, such as lipophilicity, volatility, sorption potential, and water solubility. Moreover, antibiotic soil sorption can significantly affect such vital factors as maneuverability, responsiveness to environmental elements including contaminants, as well as availability for microbial disintegration [33; 46; 47]. Soil composition including clay content, ionic strength, organic matter content, as well as pH and texture, have been determined to alter the degree and mechanism of soil sorption [48]. The research methodology employed in [PERSON]'s study in terms of the concentration of antibiotics applied to the treatment groups, compares to that used in the present study.