applied sciences  
_Theis sempervirens_: Antiproliferative Potential from Our Garden  
[PERSON] 1,*(), [PERSON] 2(), [PERSON] 3() and [PERSON] 1,*()  
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Considering the variability reported by [PERSON] et al. (2020), the estimated age of the crown group of the Iberideae tribe was established at 14.6 \(\pm\) 1.8 million years ago (mya) [10]. In the analyses of the _Iberis_ species' tmrca, the most recent common ancestor is estimated to be older than 1 mya (ranging from 1.72 to 2.44 mya) [4]. Glucosinolates (GSLs) are plant-specialized metabolites found in Brassicaceae that include sulfur and nitrogen in their structures. GSLs first appeared around 92 mya, with arylaliphatic and branched GSLs originating from phenylalanine and branched chain amino acids (valine, isoleucine, and leucine), and indolic ones appearing around 77 mya originating from tryptophan. Met-derived GSLs emerged later, ca. 61 mya, and are associated with the Brassicaceae, Cleomaceae, and Capparaceae diversification [11; 12; 13; 14]. GSLs are recognized as cancer prevention agents, biopesticides, and the source of distinctive flavor through the breakdown products they produce, mostly isothiacyanates (ITCs). To date, 90 of the 139 GSLs discovered in the plant kingdom have been fully characterized by modern spectroscopy techniques [15; 16; 17]. The distribution and level of the GSL identification in plants belonging to the _Iberis_ genus investigated to date is given in the Table 1. [PERSON] and [PERSON] in 1954 identified for the first time (\(R_{\text{S}}\))-3-(methylsulfonyl)propyl GSL in _Iberis amara_, naming it glucoiberin (**73**) [18]. Later, (\(R_{\text{S}}\))-3-(methylsulfonyl)propyl GSL was discovered by [PERSON] et al. (1955) in _I. sempervients_ and was named glucoibervirin (**95**) [19]. Several plants from the _Iberis_ genus, including _I. amara_, _I. crenata_, _I. liniifolia_, _I. sempervients_, _I. simplex_, and _I. umbellata_, were mentioned in the earliest reports of the GSLs (prior to 2000). All the GSLs identified using paper chromatography and GC-MS analysis of breakdown products were Met-derived GSLs **73**, **95**, but-3-enyl GSL (gluconapin, **12**), 3-(methylsulfonyl)propyl (glucocheinolin, **82**), 4-(methylsulfonyl)butyl GSL (glucosevicin, **84**), and prop-2-enyl GSL (sinigrin, **107**). [PERSON] et al. (2004) used the HPLC-MS technique to study the presence of GSLs in different plant seeds from the _Iberis_ genus both qualitatively and quantitatively. They confirmed the presence of Met-derived GSLs as follows (from highest to lowest, respectively): _I. amara_ (**73**, **95**), _I. compacta_ (**82**), _I. crenata_ (**82**, **73**), _I. gibrtalarica_ (**73**), _I. hybrida_ (**73**), _I. saxatilis_ (**73**), _I. sempervients_ (**84**, **95**, **73**, glucoraphanin (**64**), and **12**), and _I. umbellata_ (**73**, **107**, **95**) [20]. Using GC-MS, by their GSL breakdown products, namely but-3-enyl ITC, 4-(methylsulfonyl)butyl ITC and 5-(methylsulfonyl)pentanonitrile, 3-(methylsulfonyl)propyl ITC and 4-(methylsulfonyl)butanonitrile, and allyl ITC, [PERSON] et al. also indicated the presence of GSL **12**, **84**, **95**, and **107**, respectively [21]. Using HPLC-MS and standards, [PERSON] et al. (2017) reported that _I.