## 4 Conclusions  
In this work, a new method for the simultaneous determination of glyphosate and 13 pesticides of different chemical classes was developed. The method shows acceptable performance parameters, and is relatively straightforward in terms of operation. In the future, different ratios of sample to extraction solvent will be tested and optimized in order to achieve a better concentration factor for the SPME extraction. In soils with greater matrix interferents, it may be necessary to employ some form of clean-up of the glyphosate extract prior to derivatization. The use of SPME LC-Tips permitted the development of this combined method in a way that would have been difficult otherwise (by performing serial extractions) because the extraction of multiclass pesticides is itself performed in an aqueous solvent, whereas it normally uses an organic solvent. This new SPME configuration appears to have interesting properties and opens several avenues for further exploration, such as  
Figure 4: Sample GC-MS/MS (MRM) chromatogram from an extract of spiked soil at 60 ug/kg. 1—aminomethylphosphonic acid; 2, 3—glyphosate and isotopically labeled glyphosate (co-eluting); 4—MCPA; 5—2,4-D; 6—pentachloroanisole; 7—indane; 8—terbutylazine; 9—tefluthrin; 10—metalaxyl; 11—prousifocarb; 12, 13, 14, 15—aldrin, metolachlor, malathion, and chlorpyrifos; 16—tetraconazole; 17—metazachlor; 18, 19—isotopically labeled penconazole and penconazole; 20—buprofenzin; 21—tebuconazole; 22—diflufenican; 23—epoxiconazole; 24—boscalid.  
direct screening of soils without prior sample preparation by the insertion of the fibers onto wet soil, and then, transporting them to the laboratory.  
The following supporting information can be downloaded at: [[https://www.mdpi.com/article/10.3390/app14198584/s1](https://www.mdpi.com/article/10.3390/app14198584/s1)]([https://www.mdpi.com/article/10.3390/app14198584/s1](https://www.mdpi.com/article/10.3390/app14198584/s1)), Table S1: Multiple-reaction monitoring transitions for MS/MS experiments.  
Conceptualization P.G., E.P.M., and A.B.R.; data curation J.B.; formal analysis J.B.; funding acquisition A.B.R., P.G., E.P.M., and M.G.d.S.; investigation J.B.; methodology J.B.; project administration A.B.R., P.G., E.P.M., and M.G.d.S.; resources A.B.R., P.G., E.P.M., and M.G.d.S.; software J.B., P.G., and E.P.M.; supervision M.G.d.S., A.B.R., E.P.M., and P.G.; validation J.B.; writing--original draft J.B.; writing--review and editing A.B.R., P.G., E.P.M., and M.G.d.S. All authors have read and agreed to the published version of the manuscript.  
This work received national funds through Fundacao para a Ciencia e a Tecnologia (FCT) through the Research units CENSE \"Center for Environmental and Sustainability Research\", (PTDC/CTA-AMB/6587/2020) and LAQV/REQUIMTE (UID/QUI/50006/2020). FCT is also acknowledged for [PERSON] (UI/BD/150867/2021) fellowship, and [PERSON] established under Individual Call to Scientific Employment Stimulus (CEECIND/01969/2020). This research is anchored at RESOLUTION LAB, an infrastructure at NOVA School of Science and Technology.  
Not applicable.  
The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding authors.  
The authors declare no conflicts of interest.