## 3 Results
### Nutritional Analysis between Commercial Pellet Diet and Ex-situ Biofloc
Unlike amino acid profiles, not all of the fatty acid content of the commercial pellet was higher than that of ex-situ biofloc (Table 6). A total of 32 fatty acids (6-24) were detected in the commercial pellet, whereas all except for erucic acid (22:1n9) and Cis-13,16-docosadienoic acid (22:2) were detected in ex-situ biofloc. While both feeds share some common major fatty acids (Palmitic acid (16:0), palmitoleic acid (16:1), oleic acid (18:1n9c) and eicosapentaenoic acid (20:5n3; EPA)), other major fatty acids were different. Considering amounts of fatty acid between the commercial pellet and ex-situ biofloc, 18 fatty acids showed significantly higher levels in the commercial pellet (\(p<0.05\)); of these, four, namely oleic acid (18:1n9c, 11.69-fold increase), \(\delta\)-linolenic acid (18:3n6, 31.62-fold increase), cis-11-Eicosanoic acid (20:1, 24.22-fold increase) and DHA (22.38-fold increase) were >10 folds higher. On the other hands, only four fatty acids in ex-situ biofloc showed significantly higher levels than in the commercial pellet (\(p<0.05\)): myristoleic acid (14:1, 0.38-fold decrease), palmitoleic acid (16:1, 0.51-fold decrease), cis-8,11,14-eicosatrienoic acid (20:3n6, 0.14-fold decrease) and cis-11,14,17-eicosatrienoic acid (20:3n3, 0.71-fold decrease). \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Amino Acids (g/100 g DW)** & **Commercial Pellet (C)** & **Ex Situ-Biofloc (BF)** & **Fold Change (C/BF)** \\ \hline Alanine & 2.29 \(\pm\) 0.04 \({}^{\mathrm{a}}\) & 0.73 \(\pm\) 0.06 \({}^{\mathrm{b}}\) & 3.14 \\ Glycine & 2.95 \(\pm\) 0.09 \({}^{\mathrm{a}}\) & 0.50 \(\pm\) 0.09 \({}^{\mathrm{b}}\) & 5.90 \\ Valine & 1.76 \(\pm\) 0.06 \({}^{\mathrm{a}}\) & 0.52 \(\pm\) 0.02 \({}^{\mathrm{b}}\) & 3.38 \\ Leucine & 2.68 \(\pm\) 0.09 \({}^{\mathrm{a}}\) & 0.64 \(\pm\) 0.02 \({}^{\mathrm{b}}\) & 4.19 \\ Isoleucine & 1.55 \(\pm\) 0.06 \({}^{\mathrm{a}}\) & 0.48 \(\pm\) 0.01 \({}^{\mathrm{b}}\) & 3.23 \\ Proline & 4.63 \(\pm\) 0.12 \({}^{\mathrm{a}}\) & 0.51 \(\pm\) 0.04 \({}^{\mathrm{b}}\) & 9.08 \\ Methionine & 0.69 \(\pm\) 0.01 \({}^{\mathrm{a}}\) & 0.23 \(\pm\) 0.01 \({}^{\mathrm{b}}\) & 3.00 \\ Serine & 1.80 \(\pm\) 0.13 \({}^{\mathrm{a}}\) & 0.29 \(\pm\) 0.09 \({}^{\mathrm{b}}\) & 6.21 \\ Threonine & 1.68 \(\pm\) 0.12 \({}^{\mathrm{a}}\) & 0.34 \(\pm\) 0.08 \({}^{\mathrm{b}}\) & 4.94 \\ Phenylalanine & 1.55 \(\pm\) 0.06 \({}^{\mathrm{a}}\) & 0.46 \(\pm\) 0.02 \({}^{\mathrm{b}}\) & 3.37 \\ Aspartic acid & 2.63 \(\pm\) 0.20 \({}^{\mathrm{a}}\) & 0.77 \(\pm\) 0.27 \({}^{\mathrm{b}}\) & 3.42 \\ Hydroxyproline & 0.77 \(\pm\) 0.01 \({}^{\mathrm{a}}\) & 0.144 \(\pm\) 0.0004 \({}^{\mathrm{b}}\) & 5.35 \\ Cysteine & 0.54 \(\pm\) 0.04 \({}^{\mathrm{a}}\) & 0.053 \(\pm\) 0.003 \({}^{\mathrm{b}}\) & 10.19 \\ Glutamic acid & 5.22 \(\pm\) 0.24 \({}^{\mathrm{a}}\) & 1.44 \(\pm\) 0.39 \({}^{\mathrm{b}}\) & **3.63** \\ Lysine & 1.19 \(\pm\) 0.02 \({}^{\mathrm{a}}\) & 0.34 \(\pm\) 0.10 \({}^{\mathrm{b}}\) & **3.50** \\ Arginine & 2.89 \(\pm\) 0.06 \({}^{\mathrm{a}}\) & 0.660 \(\pm\) 0.126 \({}^{\mathrm{b}}\) & 4.38 \\ Histidine & 2.61 \(\pm\) 0.01 \({}^{\mathrm{a}}\) & 0.415 \(\pm\) 0.011 \({}^{\mathrm{b}}\) & 6.29 \\ Tyrosine & 1.27 \(\pm\) 0.05 \({}^{\mathrm{a}}\) & 0.44 \(\pm\) 0.03 \({}^{\mathrm{b}}\) & **2.89** \\ Tryptophan & 1.03 \(\pm\) 0.03 \({}^{\mathrm{a}}\) & 0.170 \(\pm\) 0.0003 \({}^{\mathrm{b}}\) & 6.06 \\ \hline \hline \end{tabular} Note: Blue-colored text indicated that these calculated values were out of limit of quantification.  
\end{table} 
Table 5: Amino acid profiles between commercial pellet and ex-situ biofloc. Different letters above the values indicate significant differences between the feeds. Letters \({}^{\mathrm{a}}\) and \({}^{\mathrm{b}}\) represent statistical analysis using student-\(t\)-test. Different letters comparing between commercial pellet and ex-situ biofloc represent significant difference (\(p<0.05\)).