# Influences of Temperature and Substrate Particle Content on Granular Sludge Bed Anaerobic Digestion
## 2 Materials and Methods
### ADM1 Simulation
\begin{table}
\begin{tabular}{l c c} \hline \hline
**Variable** & **Description and Unit** & **Value** \\ \hline input\_Qin\_dyn & Feed flow rate (m\({}^{3}\)/day) & \(3.38\times 10^{-4}\) \\ input\_S\_fa\_in & Long-chain fatty acids (kg COD-m\({}^{-3}\)) & 2.0 \\ input\_S\_aa\_in & Amino acids (kg COD-m\({}^{-3}\)) & 2.83 \\ input\_S\_IC\_in & Total inorganic carbon (M) & 0.2 \\ input\_S\_IN\_in & Total inorganic nitrogen (M) & 0.2 \\ input\_S\_I\_in & Soluble inert COD (kg COD-m\({}^{-3}\)) & 2.3 \\ input\_S\_su\_in & Monosaccharides (kg COD-m\({}^{-3}\)) & 3.6 \\ input\_X\_I\_in & Particulate inerts (kg COD-m\({}^{-3}\)) & 2.7 \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Model settings for input parameters. COD—chemical oxygen demand. \begin{table}
\begin{tabular}{l c c c} \hline \hline \multicolumn{1}{c}{**Variable**} & **Yield from Disintegration** & \multicolumn{2}{c}{**Value**} \\ \cline{2-4}  & **Model** & **ADM1** \\ \hline f\_ch\_xc1 & Carbohydrates from complex particulates (fast) & 0.2305 & \\ f\_ch\_xc2 & Carbohydrates from complex particulates (slow) & 0.2305 & \\ f\_li\_xc1 & Lipids from complex particulates (fast) & 0.0805 & \\ f\_li\_xc2 & Lipids from complex particulates (slow) & 0.0805 & \\ f\_pr\_xc1 & Proteins from complex particulates (fast) & 0.101 & \\ f\_pr\_xc2 & Proteins from complex particulates (slow) & 0.101 & \\ f\_SI\_xc1 & Soluble inerts from complex particulates (fast) & 0.0715 & \\ f\_SI\_xc2 & Soluble inerts from complex particulates (slow) & 0.0715 & \\ f\_XI\_xc1 & Particulate inerts from complex particulates (fast) & 0.0165 & \\ f\_XI\_xc2 & Particulate inerts from complex particulates (slow) & 0.0165 & \\ f\_XI\_xc2 & Particulate inerts from complex particulates (slow) & 0.0165 & \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Model settings for fast and slow disintegration parameters classified into fast and slow: ADM1 = anaerobic digestion model no.1. \begin{table}
\begin{tabular}{l c c} \hline \hline \multicolumn{1}{c}{**Variable**} & **Yield from Degradation** & \multicolumn{2}{c}{**Value**} \\ \cline{2-4}  & **Model** & **ADM1** \\ \hline f\_ac\_aa & Acetate from amino acids & 0.40 & 0.40 \\ f\_ac\_su & Acetate from sugars & 0.41 & 0.41 \\ f\_bu\_aa & Butyrate from amino acids & 0.26 & 0.26 \\ f\_bu\_su & Butyrate from monosaccharides & 0.13 & 0.13 \\ f\_fa\_li & LCFAs from lipids & 0.95 & 0.95 \\ f\_h2\_aa & Hydrogen from amino acids & 0.06 & 0.06 \\ f\_h2\_su & Hydrogen from monosaccharides & 0.19 & 0.19 \\ f\_pro\_aa & Propionate from amino acids & 0.08 & 0.05 \\ f\_pro\_su & Propionate from monosaccharides & 0.27 & 0.27 \\ f\_va\_aa & Valerate from amino acids & 0.23 & 0.23 \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Model settings for yield for acidogenesis. LCFA—long-chain fatty acid. Two modes of simulations were carried out. In Mode 1, first-order disintegration kinetics was used without classification of particulates into fast and slow disintegrating sections. In Mode 2, particulates were classified as fast and slow disintegrating. Figure 2 illustrates the two modes of simulations employed.