## 3 Results
### Laser Treatments  
With regard to laser treatments, three time intervals were used: 4 min, 16 min, and 32 min at both 5 cm and 10 cm between the laser source and the sample. It was evident that _Thermoleptolyngbya_ sp., _Leptolyngbya_ sp., and _Synechococcus elongatus_ extracts derived from cyanobacterial biomass pre-exposed to the laser and extracts directly exposed to 635 nm diode laser irradiation had enhanced antimicrobial effects against _Pantoea vagans_. Direct laser treatment of extracts resulted in a much higher inhibitory effect than that of the untreated cyanobacterial extract used as a negative control (Table 1). Effect of Laser Treatment on Antimicrobial Activity of Thermoleptolyngbya sp. Biomass Pre-Exposed to Laser and Directly Exposed Extracts against Pantoea vagans  
The extract derived from cyanobacterial biomass pre-exposed to the laser of _Thermoleptolyngbya_ sp. and its aqueous extract, which was directly exposed to the laser, showed some antimicrobial action against _Pantoea vagans_ (Table 1). _Thermoleptolyngbya_ sp. extracts derived from cyanobacterial biomass pre-exposed to the laser and extracts directly exposed to the laser were tested against _Pantoea vagans_. The inhibition zone for the negative control was 9.5 mm. At a treatment distance of 5 cm, no enhancing effect of the laser treatment was observed at any time interval, as shown in (Table 1). Similarly, the effect of extracts directly exposed to laser at 5 cm against _Pantoea vagans_ was not statistically significant. Extracts directly exposed to the laser for different time intervals did not exhibit enhanced antimicrobial action. At a treatment distance of 10 cm, the _Thermoleptolyngbya_ sp. extract derived from biomass pre-exposed to the laser against _Pantoea vagans_ exhibited similar results to those at the 5 cm distance. In contrast, the _Thermoleptolyngbya_ sp. extract directly exposed to the laser exhibited higher inhibition zone diameters for most exposure times: 15 mm for both 4 min and 32 min, more than that of the control, as shown in (Table 1). \begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{2}{*}{**Cyanobacterial Strain**} & \multirow{2}{*}{**Distance**} & \multirow{2}{*}{**Exposure Time**} & \multicolumn{2}{c}{_Pantoea vagans_} & \multicolumn{2}{c}{_Pantoea vagans_} \\  & & & & **Inhibition Zone (mm)** & **Inhibition Zone (mm)** \\  & & & & **Extract from Biomass Pre-Exposed to Laser** & **Extract Directly Exposed to Laser** \\ \hline \multirow{6}{*}{_Thermoleptolyngbya_ sp.} & 4 min & 8.5 \(\pm\) 2.1 & Nil \\ \cline{3-5}  & 5 cm & 16 min & 9.5 \(\pm\) 3.5 & 8.5 \(\pm\) 2.1 \\ \cline{3-5}  & & 32 min & 7.0 \(\pm\) 0.0 & Nil \(\pm\) 0 \\ \cline{3-5}  & \multirow{2}{*}{10 cm} & 4 min & 9.0 \(\pm\) 1.4 & 15.0 \(\pm\) 0.7 * \\ \cline{3-5}  & & 16 min & 8.0 \(\pm\) 0.0 & 11.5 \(\pm\) 2.1 \\ \cline{3-5}  & & 32 min & 9.0 \(\pm\) 1.4 & 15.0 \(\pm\) 4.2 * \\ \hline \multirow{3}{*}{_Leptolyngbya_ sp.} & 4 min & 9.5 \(\pm\) 0.7 & 9.5 \(\pm\) 0.7 \\ \cline{3-5}  & 5 cm & 4 min & 7.5 \(\pm\) 0.7 & 10.0 \(\pm\) 0.0 \\ \cline{3-5}  & 5 cm & 16 min & 8.0 \(\pm\) 1.4 & 11.0 \(\pm\) 1.4 \\ \cline{3-5}  & & 32 min & 9.5 \(\pm\) 0.7 & 13.0 \(\pm\) 0.0 \\ \cline{3-5}  & & 4 min & 9.0 \(\pm\) 1.4 & Nil \\ \cline{3-5}  &Effect of Laser Treatment on Antimicrobial Activity of Leptolyngbya sp. Biomass Pre-Exposed to Laser and Extracts Directly Exposed against Pantoea vagnas  
The extract derived from _Leptolyngbya_ sp. biomass pre-exposed to the laser and the extract directly exposed to the laser showed dissimilar antimicrobial effects against _Pantoea vagnas_ (Table 1). At a treatment distance of 5 cm, only the _Leptolyngbya_ sp. extract directly exposed to the laser showed enhanced antimicrobial activity with increasing time intervals against _Pantoea vagans_ (Table 1). At a treatment distance of 10 cm, only the _Leptolyngbya_ sp. extract directly exposed to the laser showed enhanced antimicrobial activity against _Pantoea vagans_ with 32 min exposure (Table 1).