## 3 Characterization of Molecularly Imprinted Polymer
\begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline
**Type of Poly-** & **Template** & **Functional** & **Crosslinkers** & **Initiator** & **Porogen Solvent** & **Washing Solvent** & **Ref.** \\ \hline \multirow{3}{*}{Suspension} & Diberazothiophene & 4-Vinylpyridine & Ethylenelycol & Azobissobutyronitrile & Toluene & Ethanol & [84] \\  & & & 2-Dimethyl- & & & & \\  & & & melanocyte, \(N\)- & & & & \\  & & Archylic acid, & viinylpyrrolidone, & Acetonitrile, & & & \\  & & Methacrylic acid, & Ethylenelycol & Azobissobutyronitrile & Dimethylformamide, & \(n\)-heptane/span 80 & [61] \\  & & Acrylamide & Triethylodi & & & & \\  & & & propane & & & & \\  & & & tricarylate & & & & \\  & 4- & & Ethylenelycol & & & & \\  & hydroxybenzoic acid & 4-Vinylpyridine & Ethylenelycol & Azobissobutyronitrile & Acetonitrile & Methanol/Acctic acid & [85] \\ \hline \hline \end{tabular}
\end{table} 
Table 1: \(Cont.\)pore size. Several macro-pores and flow-through channels suggests that the material was suitable for use as an SPE adsorbent. Thus, the enormous surface area and high adsorption capacity of a homogeneous macro-pore structure could theoretically be achieved. [PERSON] et al. [77] studied MIPs prepared via an oxidation-reduction polymerization system using a non-covalent molecularly imprinting strategy with hypericin as the template, acrylamide as the functional monomer, and pentaerythritl triacrylate as the crosslinker in an acetone porogen. TG and DTA (Figure 5) were used to analyze the mechanism of decomposition of a described hypericin-imprinted polymer (P1-M) in a dynamic nitrogen atmosphere. The decomposition temperature was 277.68 \({}^{\circ}\)C, and the mass-loss ratio was 99.88% when the temperature reached 455.97 \({}^{\circ}\)C, according to the TG plot. The DTA curve shows that the melting point temperature and enthalpy of melting (\(\triangle\)H) were 387.02 \({}^{\circ}\)C and 6717.36 J g\({}^{-1}\), respectively, at the starting temperature (228.48 \({}^{\circ}\)C) of the thermal absorption. SEM images of the surface morphologies of MIPs and NIPs are shown in Figure 6. The MIP particles (Figure 6a,b) were more porous, had bigger pore sizes, and had a rougher structure than the NIP (Figure 6c,d). It can be concluded that MIPs with a more uniform and open structure is significantly better for template embedding and mass transfer than NIPs. A more detailed discussion of the relationship between the pore system of different bulk imprinted polymers and their sorption properties was described by [PERSON] et al. [88] researched synthetic methods for the selective extraction of hydroxylated metabolites from human urine samples. In this study, it was observed that the specific surface area of MIP was higher than of NIP. The fact was explained by the MIP had six times the pore volume of the equivalent NIP. In addition, studies conducted by [PERSON] et al. [75] says that pore volume of NIP somewhat lower than MIP. On the contrary, [PERSON] et al. [89] studies shows that specific surface area of NIP was higher than of MIP. As, the NIP had a larger total pore volume (0.51 cm\({}^{3}\)/g) compared to the MIP (0.39 cm\({}^{3}\)/g) in conjunction with the higher SSA. The interaction of the DCF molecules as a template with MAA, which could partially inhibit crosslinking, potentially explains the MIP's lower pore volume compared to the NIP. These findings support the notion that the imprinting effect, and not the pore size distribution, is responsible for the MIP's adsorption effectiveness. Figure 5: Thermogravimetric analysis (TG) and differential thermal analysis (DTA) of hypericin-imprinted polymer [77]. The morphology of MIP and NIP particles by [PERSON] et al. [88] showed in Figure 7A shows SEM images of MIP particles made from crushed polymer monolithts generated by bulk polymerization, of which possess a non-spherical shape (A). On the imprinted Figure 7B and non-imprinted surfaces Figure 7C, the textural surface of the polymers exhibits a typical pattern (C).