## 2 Advances in Understanding Denitrification in Agricultural Soils  
This special issue of the Earth Sciences and Geography section of Applied Sciences sought to collect studies in the areas of soil denitrification and related water quality issues. This is a specific topic characterized by different aspects and facets, such as atmospheric pollution due to greenhouse gases, surface water eutrophication, groundwater pollution by nitrate, and plant-soil interactions. For the above-mentioned reasons, the papers in this special issue provide an overview of the different points of view that different disciplines can use to tackle this global issue. In fact, anthropogenic nitrogen input in the global water cycle has already been recognized as a main driver in the changing global nitrogen biogeochemistry [7]. The contribution of [PERSON] et al. [8] is on the watershed vulnerability assessment aspects of denitrification. They have analyzed four different watersheds located in the Mediterranean area (two in Italy and two in Greece), with a previously developed GIS vulnerability index called LOSN. They found that LOSN can identify portions of the landscape with higher potential denitrification directly enhancing the groundwater vulnerability assessment; although, the LOSN index only considers the topsoil, so they have incorporated it into other GIS vulnerability methods (SINTACS-SVN and DRASTIC-PA), to account for the whole aquifer vulnerability assessment. The proposed approach could be useful for a first screening in potentially vulnerable areas with scarce hydrogeological data. [PERSON] et al. [9] provided a budget approach at the basin scale to account for missing nitrogen in agricultural landscapes. They employed agricultural statistics, water quality datasets, and results of laboratory experiments to set up a detailed nitrogen budget along the terrestrial-freshwater continuum. The presented study fosters discussion on how denitrification could be considered as the leading process of nitrate removal in heavily fertilized lowland soils. [PERSON] et al. [10] worked at the plot scale, investigating the effects of zeolites (natural and amended with pig slurry) on the C and N cycles. They combined crop yield with \({}^{15}\)N isotope analyses in both soil and plants with C/N, humic substances, and fulvic acids to gain insights on the N and C turnover. In fact, this work focuses on analyzing N and C pools and \({}^{15}\)N distribution in agricultural soils with the aim of understanding the effects of zeolite addition on the soil-plant system. They found that nitrate content was lower in natural zeolitite treatments, which led to a lower nitrate availability for denitrifying bacteria. Overall, they have shown very complex interactions between soil microbes, fungi, and plants. This further promotes new studies on the effective denitrification capacity in such complex environments. [PERSON] et al. [11] assessed the N budget in agricultural plots used for short rotation forestry crops amended with biogas plant digestate. They performed an N balance by comparing a short rotation forestry with a permanent meadow, both located in an area with highly permeable soils. They used two different amounts of digestate for each system. The results obtained indicate that, in the presence of highly permeable soils, the short rotation forestry is not effective in retaining N during the initial stage of growth, due to limited denitrification rates and high leaching rates. This case study can provide valuable information for integrated soil management in upland regions characterized by highly permeable soils. [PERSON] et al. [12] presented the most bioengineering-oriented manuscript of this special issue. They isolated _Janthinobacterium svalbardensis_ F19 from sludge sediment, which is capable of simultaneously executing heterotrophic nitrification and aerobic denitrification under aerobic conditions.