## 5 Conclusions  
In the present study, the cumulative photosynthetic CO\({}_{2}\) uptake of four representative Japanese deciduous broad-leaved forest tree species was estimated from _A_-light curves measured periodically during the growing season. The whole-plant-level \(\Sigma P_{\rm n\_est}\) were highly correlated with the whole-plant dry mass increments over the two growing seasons. Because whole-plant growth is largely determined by the amount of leaf area per plant and net photosynthetic rate per leaf area, this result suggests that leaf-level \(\Sigma P_{\rm n\_est}\), which was estimated from the monthly _A_-light curves and hourly PPFD, could reflect the cumulative photosynthetic CO\({}_{2}\) uptake of the seedlings per unit LA. The \(\Sigma A_{\rm sat}\times\)days calculated from periodically measured \(A_{\rm sat}\) could be used to evaluate the O\({}_{3}\)-induced percent change in leaf-level \(\Sigma P_{\rm n\_est}\). Although the O\({}_{3}\)-induced percent changes in leaf-level \(\Sigma P_{\rm n\_est}\) were well explained by POD\({}_{0}\) in all the tree species, species-specific responses of leaf-level \(\Sigma P_{\rm n\_est}\) to POD\({}_{0}\) were observed. In addition, the flux threshold appropriate for the linear regression of the responses of relative leaf-level \(\Sigma P_{\rm n\_est}\) to POD was also species-specific. Therefore, species-specific responses of cumulative photosynthetic CO\({}_{2}\) uptake to stomatal O\({}_{3}\) uptake could be used for more accurate evaluations of O\({}_{3}\) impact on the NPP of Japanese deciduous broad-leaved forest tree species. **Supplementary Materials:** The following are available online at [[http://www.mdpi.com/1999-4907/10/7/556/s1](http://www.mdpi.com/1999-4907/10/7/556/s1)]([http://www.mdpi.com/1999-4907/10/7/556/s1](http://www.mdpi.com/1999-4907/10/7/556/s1)): Table S1: Monthly means of air temperature, vapor pressure deficit (VPD) and photosynthetic photon flux density (PPFD) inside the chambers during the O\({}_{3}\)-exposure periods in 2012 and 2013, Table S2: SUM00, AOT40 and POD\({}_{0}\) corresponding to each \(A_{\rm sat}\) in 2012 and 2013, Table S3: Results of the parameterization of the stomatal conductance models, Table S4: POD\({}_{0}\), \(\Sigma P\)\({}_{\rm n,est}\) and \(\Delta A_{\rm sat}\)\(\times\)days in 2012 and 2013, Table S5: Actual air temperatures inside the leaf chamber for measuring gas exchange rates, Table S6: Results of fitting the light-response curve of photosynthesis with non-rectangular hyperbolic function, Table S7: Light-saturated net photosynthetic rate at photosynthetic photon flux density of 1500 umd m\({}^{-2}\) s\({}^{-1}\) (\(A_{\rm sat}\), umd m\({}^{-2}\) s\({}^{-1}\)) in 2012 and 2013, Table S8: Effects of O\({}_{3}\) on the whole-plant dry mass at the end of the exposure in October and November 2013, Figure S1: Typical effect of O\({}_{3}\) on light response curve of net photosynthesis (\(A\)-light curve) observed in the present study. Conceptualization, T.I. and H.M.; Methodology, T.I. and H.M.; Investigation, H.M., M.Y. and Y.K. ; Resources, H.M.; Writing--Original draft preparation, M.Y. and Y.K. ; Writing--Review & editing, T.I. and H.M.; Visualization, M.Y. ; Supervision, T.I. Funding acquisition, T.I. This research was funded by the Ministry of the Environment, Japan, through the Program of the Environment Research and Technology Development Fund, grant number 5B-1105. The authors acknowledge [PERSON] (The University of Tokyo) for his valuable suggestions. We also acknowledge [PERSON] (Hokkaido University) and [PERSON] (Forestry and Forest Products Research Institute) for their valuable suggestions and technical support. The authors are greatly indebted to [PERSON] (Association of International Research Initiatives for Environmental Studies) for his comprehensive guidance. The authors also acknowledge [PERSON] (Civil Engineering Research & Environmental Studies) for his technical support. The authors declare no conflict of interest.