## 2 Results and Discussion
### Polyamines  
The content of total polyamines remains constant during plant development; however, they accumulate under stressful circumstances (Supplementary Figure S2). A chromatogram of benzoylated polyamine standards was obtained by reverse phase HPLC using a C18 column for separation (Supplementary Figure S3a). The peak identities were verified by injecting polyamine references into the plant tissue extract (Supplementary Figure S3b). The retention time of each peak, expressed in min, is accurate and consistently reproducible. On the left side of the chromatogram are peaks corresponding to major byproducts of the \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Rt (min)** & **Neutral Mass (Da)** & **Observed Mass m/z** & **Mass Error (ppm)** & **Adducts** & **Phenolic Compound** \\ \hline
0.82 & 558.137 & 617.149 & \(-\)3.7 & +CH3 COO & (+)-Gallocatechin hexaacetate \\ \hline
0.83 & 606.195 & 665.210 & 2.2 & +CH3 COO & 5,7-Dimethoxy-4\({}^{\prime}\)-hydroxyflavone-4\({}^{\prime}\)-O-alpha-L-rhamnose (l-\(\succ\)2)-beta-D-glucose \\ \hline
0.84 & 652.200 & 711.214 & \(-\)0.7 & +CH3 COO & Nevdensin-7-O-[a-L-rhamosyl(l-\(\succ\)6)]-beta-D-glucose \\ \hline
0.85 & 786.258 & 845.277 & 5.3 & +CH3 COO & Echinacoside \\ \hline
0.86 & 150.053 & 195.050 & \(-\)5.8 & +HCOO & Ribose \\ \hline
0.87 & 342.110 & 341.105 & 4.6 & \(-\)H, +HCOO & 4\({}^{\prime}\)_5,6,7-Tetramethoxy-flavone_ \\ \hline
0.87 & 342.116 & 387.112 & \(-\)5.8 & +HCOO & Maltose \\ \hline
0.88 & 720.211 & 719.197 & \(-\)9.7 & \(-\)H & Chelidimerine \\ \hline
0.90 & 180.063 & 179.055 & \(-\)4.1 & \(-\)H & Glucose \\ \hline
0.95 & 88.016 & 133.014 & \(-\)2.9 & +HCOO & Pyruvic acid \\ \hline
1.04 & 330.095 & 375.092 & \(-\)4.3 & +HCOO & Vanillic acid-4-O-\(\beta\)-D-glucopyranoside \\ \hline
1.10 & 129.043 & 128.035 & \(-\)1.2 & \(-\)H & 5-Oxoproline \\ \hline
1.18 & 332.074 & 331.069 & 5.7 & \(-\)H & 1-Galloy-glucose \\ \hline
1.26 & 425.045 & 424.036 & \(-\)5.2 & \(-\)H & Glucosilbin \\ \hline
1.54 & 138.032 & 137.026 & \(-\)6.4 & \(-\)H & Protocatechic aldehyde \\ \hline
1.67 & 466.111 & 511.109 & \(-\)0.3 & +HCOO & Taxofiloin-3-O-glucose \\ \hline
1.98 & 466.111 & 511.112 & 4.7 & +HCOO & (2R,3R)-Taxofilin-3\({}^{\prime}\)-O-beta-D-glucopyranoside \\ \hline
2.16 & 449.108 & 508.122 & \(-\)0.7 & +CH3 COO & Cyanidin-3-glucoside \\ \hline
2.24 & 452.132 & 497.132 & 3.4 & +HCOO & Catechin-7-O-\(\beta\)-D-glucopyranoside \\ \hline
2.35 & 304.058 & 349.057 & 0.4 & +HCOO & (2R,3R)-3,57,7,2\({}^{\prime}\)-Fentanyl hydroxyyrflavanone \\ \hline
2.44 & 180.042 & 225.042 & 5.6 & +HCOO & 3,4-Dihydroxycinamic acid \\ \hline
2.58 & 402.153 & 401.143 & \(-\)5.7 & \(-\)H, +HCOO & Benzyl alcohol xylopyranosyl(l-\(\sim\)6)-glucopyranoside \\ \hline
2.60 & 330.095 & 329.089 & 3.6 & \(-\)H & Vanillic acid-4-O-\(\beta\)-D-glucopyranoside \\ \hline
2.69 & 326.100 & 385.115 & 2.8 & +CH3 COO & 4-O-\(\beta\)-D-Glucopyranosyl-\(cis\)-cinamic acid \\ \hline
3.66 & 312.121 & 357.120 & 3.7 & +HCOO & Pisidic aciddiethyl ester \\ \hline
4.13 & 326.137 & 371.135 & 0.4 & +HCOO & Eugenyl glucoside \\ \hline
4.29 & 396.142 & 395.135 & 1.1 & \(-\)H & Proyl chlorogenate \\ \hline
5.96 & 448.137 & 447.131 & 1.9 & \(-\)H & (2S)-5/5-Dihydroxy-6-methoxy-flavanone-7-O-\(\beta\)-D \\ \hline
7.02 & 316.261 & 315.252 & \(-\)7.2 & \(-\)H & Dihydroxy stearic acid \\ \hline
8.37 & 598.314 & 597.305 & \(-\)3.3 & \(-\)H & Daturametelin H \\ \hline
9.44 & 282.256 & 281.248 & \(-\)2.3 & \(-\)H & (E)-9-Octadecenoic acid \\ \hline
10.72 & 414.386 & 473.403 & 6 & +CH3 COO & 24-Ethyl cholesterol \\ \hline
12.25 & 326.188 & 325.15 & 9.7 & \(-\)H & Pregna-4,16-diene-3,12,20-trione \\ \hline \hline \end{tabular}
\end{table} 
Table 3: UPLC/QTOF-MS data of the polyphenonic compounds in _Moringa oleifera_ seedlings. benzoyl reaction that were eluted and separated from benzoylated polyamines. Putrescine (Put), spermidine (Spd), and spermine (Spm) are the three naturally occurring amines (Mol. wt. 202.34).