# AIP Bioluminescence for Rapid and Selective Detection of Bacteria and Yeasts in Wine
## 3 Results
\begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline
**Inoculum** & \multicolumn{3}{c}{**Yeast (10\({}^{4}\)) + Bacteria (10\({}^{7}\))**} & \multicolumn{3}{c}{**Yeast (10\({}^{4}\)) + Bacteria (10\({}^{3}\))**} \\ \hline
**Filter** & \multicolumn{3}{c}{**1.2 \(\upmu\)m**} & \multicolumn{3}{c}{**0.45 \(\upmu\)m**} & \multicolumn{3}{c}{**1.2 \(\upmu\)m**} & \multicolumn{3}{c}{**0.45 \(\upmu\)m**} \\ \hline
**Analysis** & \begin{tabular}{c} **RLU** \\ **YPDch** \\ \end{tabular} & SD & \begin{tabular}{c} **RLU** \\ **MRScy** \\ \end{tabular} & SD & \
\begin{tabular}{c} **RLU** \\ **YPDch** \\ \end{tabular} & SD &
\begin{tabular}{c} **RLU** \\ **MRScy** \\ \end{tabular} & SD \\ \hline R1 & \(\mathbf{7.04\times 10^{6}}\) & \(2.24\times 10^{4}\) & \(\mathbf{1.61\times 10^{6}}\) & \(6.03\times 10^{4}\) & \(\mathbf{9.23\times 10^{5}}\) & \(8.84\times 10^{4}\) & \(1.01\times 10^{3}\) & \(2.91\times 10\) \\ R2 & \(\mathbf{3.86\times 10^{6}}\) & \(3.32\times 10^{4}\) & \(\mathbf{1.14\times 10^{6}}\) & \(2.24\times 10^{4}\) & \(\mathbf{7.66\times 10^{6}}\) & \(2.14\times 10^{4}\) & \(\mathbf{1.01\times 10^{5}}\) & \(4.28\times 10^{3}\) \\ R3 & \(\mathbf{4.28\times 10^{6}}\) & \(2.96\times 10^{4}\) & \(\mathbf{1.43\times 10^{6}}\) & \(6.24\times 10^{4}\) & \(\mathbf{9.40\times 10^{6}}\) & \(4.05\times 10^{4}\) & \(1.11\times 10^{3}\) & \(2.36\times 10^{2}\) \\ R4 & \(\mathbf{3.02\times 10^{6}}\) & \(2.13\times 10^{4}\) & \(\mathbf{9.23\times 10^{5}}\) & \(1.90\times 10^{4}\) & \(\mathbf{7.38\times 10^{6}}\) & \(4.11\times 10^{4}\) & \(1.14\times 10^{3}\) & \(8.55\times 10\) \\ R5 & \(\mathbf{1.29\times 10^{6}}\) & \(1.89\times 10^{3}\) & \(\mathbf{4.11\times 10^{5}}\) & \(4.83\times 10^{3}\) & \(\mathbf{3.75\times 10^{6}}\) & \(1.87\times 10^{4}\) & \(\mathbf{1.37\times 10^{6}}\) & \(9.89\times 10^{3}\) \\ R6 & \(\mathbf{1.45\times 10^{6}}\) & \(4.88\times 10^{4}\) & \(\mathbf{9.58\times 10^{5}}\) & \(5.23\times 10^{4}\) & \(\mathbf{4.84\times 10^{6}}\) & \(3.76\times 10^{4}\) & \(\mathbf{5.70\times 10^{5}}\) & \(1.57\times 10^{4}\) \\ W1 & \(\mathbf{5.25\times 10^{6}}\) & \(5.91\times 10^{4}\) & \(\mathbf{1.32\times 10^{6}}\) & \(7.58\times 10^{4}\) & \(\mathbf{6.80\times 10^{6}}\) & \(1.97\times 10^{4}\) & \(\mathbf{1.57\times 10^{4}}\) & \(1.45\times 10^{2}\) \\ W2 & \(\mathbf{3.96\times 10^{6}}\) & \(1.20\times 10^{4}\) & \(\mathbf{4.91\times 10^{5}}\) & \(7.99\times 10^{3}\) & \(\mathbf{7.35\times 10^{6}}\) & \(5.69\times 10^{4}\) & \(1.02\times 10^{3}\) & \(8.59\times 10\) \\ W3 & \(\mathbf{4.34\times 10^{6}}\) & \(4.05\times 10^{4}\) & \(\mathbf{1.47\times 10^{6}}\) & \(4.48\times 10^{4}\) & \(\mathbf{8.07\times 10^{6}}\) & \(6.91\times 10^{4}\) & \(\mathbf{1.24\times 10^{4}}\) & \(1.41\times 10^{2}\) \\ W4 & \(\mathbf{9.69\times 10^{6}}\) & \(3.19\times 10^{4}\) & \(\mathbf{1.33\times 10^{6}}\) & \(6.91\times 10^{4}\) & \(\mathbf{3.38\times 10^{6}}\) & \(3.01\times 10^{4}\) & \(\mathbf{1.31\times 10^{6}}\) & \(2.82\times 10^{4}\) \\ W5 & \(\mathbf{3.91\times 10^{6}}\) & \(1.63\times 10^{4}\) & \(\mathbf{2.19\times 10^{4}}\) & \(5.05\times 10^{3}\) & \(\mathbf{7.75\times 10^{6}}\) & \(9.16\times 10^{4}\) & \(\mathbf{1.80\times 10^{5}}\) & \(2.48\times 10^{3}\) \\ W6 & \(\mathbf{1.74\times 10^{6}}\) & \(1.44\times 10^{4}\) & \(\mathbf{4.93\times 10^{5}}\) & \(1.04\times 10^{4}\) & \(\mathbf{8.04\times 10^{6}}\) & \(5.14\times 10^{4}\) & \(\mathbf{5.39\times 10^{5}}\) & \(2.28\times 10^{3}\) \\ Blank & \(4.24\times 10^{3}\) & \(6.55\times 10^{2}\) & \(3.65\times 10^{3}\) & \(3.16\times 10^{2}\) & \(4.04\times 10^{3}\) & \(1.10\times 10^{3}\) & \(2.03\times 10^{3}\) & \(7.12\times 10^{2}\) \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Bioluminescence assay (RLU). Sample RLU values 3 times higher than the blank RLU value indicate contamination (by yeasts when YPDch is used as resuscitation medium for 1.2 \(\upmu\)m filters, bacteria when MRScy is used to resuscitate cells on 0.45 \(\upmu\)m filters) and are indicated in bold.