## 4 Conclusions  
The present study has conclusively demonstrated the exceptional dye decolorization capabilities of strain Q3-6, achieving remarkable efficiencies of 93.9% for Brilliant blue G-250 (0.1 g/L) and 91.9% for Congo Red (0.1 g/L). Furthermore, this strain has exhibited resistance to a wide array of antibiotics and heavy metals, likely due to its robust biofilm adsorption capacity. These findings highlight the broad biotechnological potential of strain Q3-6, particularly in the bioremediation of sewage treatment and the management of complex dyeing and printing effluents.  
The encoded heat shock proteins within the genome of strain Q3-6 confer it with notable heat resistance, which is crucial for its survival and functionality in various environmental conditions. Additionally, the strain's strong spore production ability and heat-resistant properties make it a promising candidate for industrial mass production applications.  
Overall, this study underscores the significant contributions of strain Q3-6 to the field of bioremediation and wastewater treatment. Its unique combination of dye decolorization prowess, antibiotic and heavy metal resistance, and heat tolerance warrants further exploration for potential commercial and environmental applications.  
The following supporting information can be downloaded at [[https://www.mdpi.com/article/10.3390/app14167181/s1](https://www.mdpi.com/article/10.3390/app14167181/s1)]([https://www.mdpi.com/article/10.3390/app14167181/s1](https://www.mdpi.com/article/10.3390/app14167181/s1)), Table S1: Prediction of secondary metabolite synthesis gene clusters in the genome of strain Q3-6; Table S2: Reagent strip biochemical analysis experiments for strain Q3-6 (\"+\" for positive, \"\(-\)\" for negative); Table S3: The reference standards for the antimicrobial range of the disk method for drug susceptibility testing of resistant bacteria; Table S4: Antibiotic susceptibility testing of strain Q3-6 (\"R\" for resistant, \"I\" for intermediate, and \"S\" for susceptible); Figure S1: Genome-wide view of strain Q3-6; Figure S2: The secondary metabolite gene cluster of the strain; Figure S3: Scanning electron micrograph of cells of strain Q3-6. Bar, 5 \(\upmu\)m.  
Conceptualization, Y.-H.Y.; methodology, Y.-H.Y.; validation, Y.-H.Y. and H.-Y.Z.; investigation, Y.-H.Y., H.-Y.Z., B.P. and Z.-W.W.; resources, M.-Q.Y.; data curation, Y.-H.Y.; writing--original draft preparation, Y.-H.Y.; writing--review and editing, M.-Q.Y.; visualization, Y.-H.Y., B.P. and Z.-W.W.; supervision, M.-Q.Y.; project administration, Z.-J.D. and M.-Q.Y.; funding acquisition, M.-Q.Y. All authors have read and agreed to the published version of the manuscript.  
Figure 11: Effect of Brilliant blue G-250 dye on biofilm production (different lowercase letters represent significant differences (\(p<0.05\))).  
**Funding:** This work was supported by the National Natural Science Foundation of China (32200003), the Natural Science Foundation of Shandong Province (ZR2022 QC106), and the Guangdong Basic and Applied Basic Research Foundation (2022A1515110773).  
**Institutional Review Board Statement:** Not applicable.  
**Informed Consent Statement:** Not applicable.  
**Data Availability Statement:** Data is contained within the article or Supplementary Material. The GenBank accession number of _Bacillus thuringiensis_ Q3-6 for the 16S rRNA gene sequence is PP567308, for the whole-genome assembly is PRJNA1093269.  
**Conflicts of Interest:** The authors declare no conflicts of interest.