viridulum_ baker are significantly positively correlated with the release of ocimene and myrcene [14], the overexpression of _CpTPSS4_, 6, 7, 13, 14, 15, and 16 confirmed obvious upregulation of terpene content in _Chimonanthus praecox_ L. [15], while in _Freesia \(\times\) hybrida_, _FhTPS1_ is responsible for the formation of linalool [16]. Compared with the control group, transient convey of _SoTPS2_ and _SoTPS3_ in the petals of _Antitrhium majus_ increased the release of ocimene by 10.91 times and 23.67 times. However, GGPPS and TPS belong to a family of proteins, and more expression characteristics and their relationship with terpenes still need to be clarified. By overexpressing _VvTPS59_ in _V. quinuquangularis_, a significant increase in the levels of linalool, linalool oxide, geraniol, and citronellol was found, suggesting that _TPS59_ plays a role in the synthesis of linalool and its derivatives in _Vitis vinifera_[17]. [PERSON] showed [18] that the overexpression of _LiMCT_ in _Arabidopsis thaliana_ affected the expression levels of genes of the MEP and MVA pathways, suggesting that the overexpression of _LiMCT_ in _Arabidopsis thaliana_ affects the metabolic flow of the C5 precursor of two different terpene synthesis pathways. The expression of the monoterpene synthase _AtTPS14_ was nearly four-fold higher in transgenic _Arabidopsis thaliana_ flowers compared with the control, suggesting that _LiMCT_ plays an important role in regulating monoterpene synthesis and synthesis of other isoprenoid-like precursors in transgenic _Arabidopsis thaliana_ flowers. Many terpenids are also important components of chili fruit aroma. An analysis of the components of red pepper fruit revealed that terpenoids, including carvacene, (_4 aScis)-2,4a,5,6,7,8,9,9a-octahydro-3,5,5-trimethyl-9-methylene-1H-benzylidene, and _cis-(-)-2,4a,5,6,9a-hexahbro-3,5,5,9-tetramethyl-1H-benzocyclloheptene, were the main aroma components [19]. HS-SPME-GC-MS analysis of volatile aroma components in the fruits of four different chili peppers at different developmental stages showed that terpen such as merididol, linalool, \(\alpha\)-muroclene, _ar_-himachalene, and _cis-\(\alpha\)_-bergamot were higher in content. Among them, the contents of merididol and linalool are higher in the fruits of \(C\). _annuum_, while the contents of \(\alpha\)-muroclene, _ar_-himachalene, and _cis-\(\alpha\)_-bergamotene were higher in the fruits of \(C\). _chinense_, showing significant inconsistency [20]. However, there is little research on the genes and regulatory mechanisms involved in the formation of terpen in chili fruits, which hinders the revelation of differential mechanisms in terpenoid synthesis and molecular breeding for chili fragrance. Early transcriptome sequencing combined with untargeted metabolomics analysis revealed three genes encoding _DXS_, three genes encoding _HMGS_, and sixteen genes encoding _TPS_, which may be related to the formation of terpenoids. The aim of this study is to further analyze the bioinformatics characteristics of terpenoid pathway genes within the genome, their expression levels during pepper fruit development, and their relationship with the content of major terpenoids, laying the foundation for discovering key genes and their applications.