In addition, when increasing the MCC content from 2.5% to 5% (\(w/w\) of starch), the tensile strength of the starch film increased from 0.96 to 1.11 MPa while their elongation at break decreased from 76.10% to 11.36% [12]. Not only food packaging but medical packaging is also one of the large waste from plastic, which also needs to be replaced by biodegradable materials [13]. Certainly, the issue of medical packaging waste is significant and warrants attention, especially considering the environmental impact of plastic waste. Transitioning from traditional plastic medical packaging to biodegradable alternatives can help mitigate the environmental impact of plastic waste, reduce landfill usage, align with regulatory requirements and sustainability goals, and enhance public perception of healthcare organizations' environmental stewardship efforts. It represents a proactive step towards creating a more sustainable and environmentally responsible healthcare industry. Currently, there are no reports on biocomposite film for medical packaging applications due to the difficulties in the sterilization process, as it is sensitive to temperature and moisture [14]. Therefore, the development of biocomposite films and suitable sterilization methods remains a challenging task. Sterilization stands as a pivotal process in the manufacturing of medical packaging. It ensures that packaging materials and components are devoid of harmful microorganisms, maintaining their sterility until they are employed [15]. The existing literature extensively documents that traditional sterilization methods like steam, gamma irradiation, and ethylene oxide can lead to damage, alterations, or the release of toxic substances, primarily because of the thermal and hydrolytic sensitivity inherent in biodegradable materials [14]. UV-C sterilization can indeed be considered an alternative method for sterilizing film packaging. Generally, UV-C sterilization is a method used to sterilize medical devices and pharmaceutical products that are sensitive to temperature and moisture [16]. UV-C sterilization proves most effective at a wavelength of 254 nm, closely aligning with the peak absorption point of DNA. When bacteria or pathogens encounter UV-C radiation, their cells and genetic material sustain damage, disrupting their ability to replicate. UV-C radiation poses detrimental effects on a wide range of microorganisms, encompassing bacteria, bacterial spores, molds, mold spores, viruses, algae, and yeast [17]. UV-C sterilization is effective for air and surface disinfectants, but it may have limited penetration depth and may not be suitable for densely packed or opaque materials [18]. [PERSON] et al. (2008) report that UV-C can have efficient inactivation of bacteria with an exposure time of 30 min [19]. However, the longer UV-C exposure time (3 h) significantly reduced the mechanical properties of sterilized materials [20]. In this study, biocompatible films based on cassava starch and MCC extracted from cassava pulp were developed for medical packaging applications. MCC extracted from cassava pulp has received considerable interest in the development of biocomposite films because of its distinctive mechanical characteristics, non-toxic and biodegradability [21; 22]. Cassava pulp has been recognized for its significant cellulose content, reaching up to 20%, making it a suitable source for producing MCC [22]. It is obtained from the residual material of the cassava plant, one of the most important harvests in terms of annual production. However, its waste, around 7.3 million tons/year in Thailand, is rather significant, which can contribute to environmental issues [23]. The extraction of cassava pulp is one of the solutions to achieve effective use of natural resources and sustainable management, and cassava pulp became a rich source for the production of MCC without shortage. Although there have been documented successes in extracting MCC from cassava pulp, the exploration of its application in starch film reinforcement remains relatively unexplored [24; 25].