# Analysis of the Sugarane Biomass Use to Produce Green Hydrogen: Brazilian Case Study
## 1 Introduction
[PERSON] et al. (2023) extended this idea by investigating the enzymatic saccharification and dark fermentation of wheat straw, providing valuable insights into how similar processes can be applied to sugarcane biomass for higher biohydrogen production [20]. Similarly, [PERSON] et al. (2023) optimized fermentable sugar production from Napier grass for biohydrogen generation, contributing to the broader understanding of how grasses and other lignocellulosic feedstocks can be utilized for biohydrogen production, including sugarcane [21]. Several studies have explored the synergy between pretreatment methods, with some focusing on the combination of acid, enzymatic, and oxidative treatments. [PERSON] et al. (2020) investigated how alkaline and oxidative pretreatment of corn cob waste improved biohydrogen production through dark fermentation, with potential applications for sugarcane leaves and other agricultural residues [22]. [PERSON] et al. (2024) provided a comprehensive review of recent trends in dark fermentation for biohydrogen production, emphasizing the need for optimizing the transition to a circular bioeconomy through sustainable biomass utilization [23]. The role of dark fermentation in biohydrogen production was further explored by [PERSON] et al. (2020), who examined the microbial dynamics of dark fermentation and how the electron-equivalent balance can be optimized for lignocellulosic feedstocks, including sugarcane [24]. The interplay between microbial populations and the fermentation process is essential for maximizing biohydrogen yields. [PERSON] et al. (2020) also studied biohydrogen production from cotton waste and found that adjusting fermentation conditions could significantly improve hydrogen yields, suggesting similar approaches for sugarcane biomass [25]. Moreover, the utilization of fruit and vegetable waste in dark fermentation was explored by [PERSON] et al. (2020), who demonstrated how biohydrogen can be efficiently produced from such organic wastes, further emphasizing the potential for integrating sugarcane biomass into similar biorefinery systems [26]. Their work reinforces the idea that diverse agricultural residues, including sugarcane, can be incorporated into integrated waste-to-energy processes that produce biohydrogen alongside other biofuels like biogas. The integration of biohydrogen production with other renewable energy processes is another key theme in literature. [PERSON] et al. (2023) reviewed biological hydrogen production from biowaste, focusing on storage and transportation aspects, which are crucial for optimizing the biohydrogen supply chain [27]. This concept of multi-product biorefineries is increasingly relevant for sugarcane biomass, as it allows for the co-production of biohydrogen, bioethanol, and biogas, offering a more efficient use of agricultural residues. Several studies have also emphasized the potential of optimizing pretreatment methods to enhance biohydrogen production. For example, [PERSON] et al. (2022) examined deep eutectic solvents and enzymatic hydrolysis to improve the fermentability of sugarcane leaves, leading to increased biohydrogen yields through dark fermentation [36]. This work aligns with the findings of [PERSON] et al. (2022), who optimized alkaline and oxidative pretreatment methods for biohydrogen production from sugarcane bagasse, highlighting the importance of pretreatment for maximizing sugar recovery [38]. On the other hand, [PERSON] et al. (2020) demonstrated that acid addition in liquid hot water pretreatment could significantly improve biohydrogen and biogas production from lignocellulosic biomass, including sugarcane bagasse [62]. Their findings point to the need for refining pretreatment processes to increase the accessibility of fermentable sugars, an essential step for improving biohydrogen production. [PERSON] et al.