## 2 Results and Discussion
### Delivery System
SynPG-1 was able to retain activity in 25% serum for up to 24 h, compared to 2 h for the original PG-1 AMP, with MRSA biofilm being prevented by both AMPs. [PERSON] et al. described a simple strategy for the surface-active factor functionalization of biomaterials using composite biofilms of graphene oxide (GO), poly(lactide-co-glycolide) (PLGA), AMP pomericin G1, and basic fibroblast growth factor (bFGF) [32]. This composite increased the rate of in vivo wound healing and antibacterial effects. The delivery of a custom-designed AMP (W379) via a Janus-type antimicrobial dressing with dissolvable microneedle arrays was found to generate more efficient delivery than a free AMP. Notably, the dressing completely removed dual species MRSA and _P. aeruginosa_ biofilm from an ex vivo human skin model [33]. The AMP E6 was used by [PERSON] et al. in combination with the biocide tetrakis hydroxymethyl phosphonium sulfate (THPS) to inhibit the biocorrosion of EH36 ship steel [34]. Although E6 alone was not biocidal and could not prevent the binding of _Desulfoibrio vulgaris_, the AMP appeared to enhance the bactericidal effect of THPS. Research into AMP-based coatings and implant publications were also quite popular, including a combinatorial approach to antibiotics by developing a method to immobilize nisin on NO-releasing silicone rubber (SR-SNAP-Nisin), which exhibited in vitro antiinfection activity against planktonic and adherent-state _S. aureus_ and _E. coli_[35]. Elastin-like recombinamers (ELRs) were used by [PERSON] et al. to develop an extracellular matrix-mimicking system for anchoring AMPs (e.g., D-GL13K) covalently to implant coatings with strong antibiofilm activity [36]. Mesoporous titania-covered titanium implants were used to deliver RRP9W4N, generating the sustained release of the AMP with equivalent or greater antib Biofilm properties than the cloxacillin antibiotic. An additional in vivo study using a rabbit tibia model indicated no negative effects on osseointegration [37]. Immobilisation of the novel AMP JIChis-2 on the Ti-6 Al-4V alloy resulted in a functional material with antibiofilm capacities [38], while late-sporulin-coated titanium generated resistance against _S. aureus_ biofilms [39]. [PERSON] et al. impregnated polymer polydimethylsiloxane (PDMS) with r(P)ApoBLPro--an AMP associated with human apolipoprotein B [40]. The stability, peptide release and biocompatibility were evaluated with 70% of the AMP being released after 400 min and biofilm formation by _E. coli_ ATCC 25922 being prevented. Several publications described new approaches to AMP (conjugate) synthesis for AMP delivery. [PERSON] et al. reported the design of a novel neolignan isomagnolone and isomer and their conjugation to form AMP-mimic conjugates (III5 and III15), which exhibited in vitro and in vivo anti-MRSA activity comparable with vancomycin [41]. Cholesterol-modified AMP PMAP-37(F34-R), yielded Chol-37(F34-R), with increased hydrophobicity, thereby potentially increasing bacterial membrane damage and AMP stability. Subsequently, in an _S. aureus_ (ATCC 25923) mouse model of peritonitis, Chol-37(F34-R) reduced organ injury and the burden of bacteria [42]. Finally, the potential of 'click' chemistry to generate covalent AMP-ionic liquid-based conjugates was described by combining a classical imidazolium IL with the N-terminus of AMP 3.1-PP4 (MeIm-3.1-PP4). This approach maintained the AMPs' antibacterial activity for a range of pathogenic bacteria, as well as the AMP conjugate's antibiofilm proliferation activity against an MDR clinical isolate of _K. pneumoniae_, thereby demonstrating improved stability towards tyrosinase-mediated AMP modifications (of relevance, for example, in skin wounds) [43]. Efficient AMP delivery and release is essential in helping target bacteria and biofilms at active concentrations, while helping protect AMPs from rapid degradation. Encapsulation in particles, advances in coatings, and novel methods for AMP (conjugate) synthesis may help to achieve this increased efficiency. Finally, [PERSON] et al.