## 3 Results
### Histopathology and Ultra-Structural Studies
Prominent glycogen content was also evident (Figure 3H). The positive control (EO) exhibited structures similar to that of the control, demonstrating mitochondrion cristae and abundant RER (Figure 3D,E). Light microscopy revealed conspicuous deformities in the nucleus, along with the presence of a large number of cytoplasmic vacuolation and necrotic bodies in hepatocytes after 60 days of MG treatment (Figure 4A,B). At cellular level, extensive vacuolization and diminished glycogen content occurred in hepatocytes. Cellular organelles underwent modifications in the form of RER vesiculation and fragmentation of the Golgi complex (Figure 4C,D). Hepatocytes demonstrated lost integrity of tight junctions (Figure 4E). Mitochondria swelling and the abundance of lysosomes and autophagy-lysosomes indicated debilitated organelles upon MG exposure (Figure 4C-E). The inclusion of _E. officinalis_ diet in MG intoxicated fish might have protected liver architecture and maintained glycogen content (Figure 4J-L). Ultra-structural examination demonstrated revived microvilli structure and number in Bile canaliculi (Figure 4L). Space of Disse showed the existence of the phagosome in consort with lysosomes functioning for the removal of waste (Figure 4J,K). Plant extract supplementation seemed to possess no negative impact (Figure 4F-H), depicting  
Figure 3: Alterations in hepato-architecture of _C. carpio_ after exposure to malachite green and _E. officinalis_ for 30 days. MG: (**A**) (40\(\times\))—Liver with nuclear alteration such as karyolysis (KL), hypertrophied cells (HT) and congested vessel (cV) and cytoplasmic vacuolation (V). (**B**): Bile canaliculi displayed decreased Microvilli (Mi) count and abundant membrane whorls, phagolysosomes (PLy) digesting various organelles, scarcity of glycogen content (dGY) and mitochondria (M). (**C**): Lipid deposits (L), lysosomes (Ly), macrophage (Mc), phagosomes (Ph), deformed nucleus (dN) and its membrane (dNm) in hepatocytes. _E. officinalis_ alone: (**D**): regular hepato-architecture, mitochondrion with cristae (Cr), glycogen (GY) and large number of RER strands. (**E**) (40\(\times\))—Hepucytes (H) with interspersed sinusoids (S). MG _+ E. officinalis_: (**F**) (40\(\times\))—Karyolysis (Kl) and erythrocyte infiltration (EI). (**G**): Clumped mitochondria (cM), proliferated RER. (**H**): Dissolving mitochondrion cristae (dM), membrane whorls (Mw) in space of Disse and restored glycogen content (GY). regular hepatocytes (H) with interspersed sinusoids (S). Moreover, TEM study sustained the histological observations by the appearance of euchromatic nucleus, intact RER (Figure 4G); sinusoids bearing macrophage cells and nucleated erythrocytes (Figure 4H). Statistical semi-quantitative analysis of the histopathological alteration index (HAI) was made by scoring alterations as reversible, irreversible, and circulatory following [22], and this is presented in Figure 5F. Figure 4: Alterations in _C. carpio_ hepato-architecture after exposure to malachite green and _E. officinalis_ for 60 days. MG: (**A**) (100\(\times\)) and (**B**) (40\(\times\))—Prominent nuclear alterations such as pyknosis (Py), karyolysis (KI) and cytoplasmic vacuolation (V) and necrotic hepatocytes (Nc). (**C**): vesiculated RER (vRER), vacuolation (V), auto-phagolysosome (APLy), fragmented GC (fGC) and depleted glycogen content (dGY). (**D**): RER transformation into dilated RER (dRER) and vesiculated RER (vRER), prominent lysosomes (Ly) and highly swollen mitochondria (sM). (**E**): Compact nucleus (cN), lost tight junction integrity (ITJ), sparse distribution of glycogen (dGY), vacuolation (V) and pyknosis (Py). _E. officinalis_: (**F**) (40\(\times\))—Hepatocytes (H) with interspersed sinusoids (S). (**G**): Regular hepatocyte architecture comprising nucleolus (Nu) in nucleus (N), RER extension from nuclear membrane, mitochondria (M) in vicinity and glycogen abundance (GY). (**H**): Sinusoid (S) showing macrophage with lysosomes (Ly) and erythrocyte (E) with central nucleus (N). MG + _E.