# In Vivo Biosynthesis of Au and Ag NPs Using Two _Medicago sativa_ L. Genotypes
## 3 Results
### Obtaining Plant Suspension from Seedings for Spectrophotometry
The results of light absorption for the _'Kometa'_ genotype after 48 h of exposure to AgNO3 and HAuCl4 are shown in Figure 3.  
The most significant changes in the light absorption results compared to the control for the _'Kometa'_ genotype after 48 h of exposure to the precursors are summarised in Table 3. \begin{table}
\begin{tabular}{l l l} \hline
**Precursor** & **Precursor Concentration (mg/L)** & **Light Wavelength (nm)** & **Absorption (u.a.) Values** \\ \hline  & & 340–448, 451 & \textgreater{}5.00 \\ \cline{3-4}  & & 449 & 4.79 \\ \cline{3-4} Control & & 450 & 4.31 \\ \cline{3-4}  & & 452–899 & \(\downarrow\) \\ \cline{3-4}  & & 900 & 0.78 \\ \hline  & & 340–364, 366, 433–440 & \textgreater{}5.00 \\ \cline{3-4}  & & 365 & 4.79 \\ \cline{3-4}  & 50 & 367–432 & \(\downarrow\) \\ \cline{3-4}  & & 441–899 & \(\downarrow\) \\ \cline{3-4}  & & 900 & 0,61 \\ \cline{3-4}  & & 340–355, 359 & \textgreater{}5.00 \\ \cline{3-4} HAuCl4 & & 356–358 & 4.79 \\ \cline{3-4}  & 100 & 360–899 & \(\downarrow\) \\ \cline{3-4}  & & 900 & 0.54 \\ \cline{3-4}  & & 340–345, 447 & \textgreater{}5.00 \\ \cline{3-4}  & 200 & 346 & 4.79 \\ \cline{3-4}  & & 348–899 & \(\downarrow\) \\ \cline{3-4}  & & 900 & 0.58 \\ \hline  & & 340–341 & **4.79** \\ \cline{3-4}  & & 342–345 & 4.31 \\ \cline{3-4}  & 250 & 346 & 4.09 \\ \cline{3-4}  & & 345–899 & \(\downarrow\) \\ \cline{3-4}  & & 900 & 0.52 \\ \cline{3-4}  & & 340–352 & \textgreater{}5.00 \\ \cline{3-4}  & 1000 & 353–899 & \(\downarrow\) \\ \cline{3-4}  & 900 & 0.99 \\ \hline \end{tabular}
\end{table} 
Table 2: Highest and lowest light absorbance values in _’la Bella’_ after 24 h of AgNO3 and HAuCl4 exposure (green indicates results with maximum absorbance values (5.00), yellow indicates the results in which the absorption values are the highest but do not reach the maximum possible value (5.00), an arrow means a decrease in absorption results at the indicated wavelength of light). \begin{table}
\begin{tabular}{l l l l} \hline
**Precursor** & **Precursor Concentration (mg/L)** & **Light Wavelength (nm)** & **Absorption (u.a.) Values** \\ \hline \multirow{4}{*}{Control} & & **340–357, 359** & **>500** \\ \cline{3-4}  & & 358 & 4.79 \\ \cline{3-4}  & & 350–899 & \(\downarrow\) \\ \cline{3-4}  & & 900 & 0.49 \\ \hline \multirow{4}{*}{HAuCl\({}_{4}\)} & & **340–499** & **>5.00** \\ \cline{3-4}  & 50 & 500–899 & \(\downarrow\) \\ \cline{3-4}  & & 900 & 1.2 \\ \cline{3-4}  & & **340–456, 359** & **>5.00** \\ \cline{3-4}  & 100 & 457–358 & \(\downarrow\) \\ \cline{3-4}  & & 360–899 & \(\downarrow\) \\ \cline{3-4}  & & 900 & 0.48 \\ \cline{3-4}  & & **340–483** & **>5.00** \\ \cline{3-4}  & 200 & 484–899 & \(\downarrow\) \\ \cline{3-4}  & & 900 & 0.77 \\ \hline \multirow{4}{*}{AgNO\({}_{3}\)} & & **340–345** & **>5.00** \\ \cline{3-4}  & 250 & 345–899 & \(\downarrow\) \\ \cline{3-4}  & & 900 & 0.83 \\ \cline{3-4}  & & **340–343** & **>5.00** \\ \cline{3-4}  & 500 & 344–899 & \(\downarrow\) \\ \cline{3-4}  & & 900 & 0.55 \\ \cline{3-4}  & & **340–354** & **>5.00** \\ \cline{3-4}  & 1000 & 355–899 & \(\downarrow\) \\ \cline{3-4}  & & 900 & 0.56 \\ \hline \end{tabular}
\end{table} 
Table 3: Highest and lowest light absorbance values in ‘_Kometa_’ after 48 h of AgNO\({}_{3}\) and HAuCl\({}_{4}\) exposure (green indicates results with maximum absorbance values (5.00), yellow indicates the results in which the absorption values are the highest but do not reach the maximum possible value (5.00), an arrow means a decrease in absorption results at the indicated wavelength of light). Figure 3: The results of light absorption for the ‘_Kometa_’ genotype after 48 h of exposure to AgNO\({}_{3}\) and HAuCl\({}_{4}\). The results of light absorption for the _'la Bella'_ genotype after 48 h of exposure to AgNO\({}_{3}\) and HAuCl\({}_{4}\) are shown in Figure 4.  
The