## 3 Results
### Analysis of the Sequences of Biopeptides Detected in the Hydrolysates
\begin{table}
\begin{tabular}{c c c c c} \hline \hline  & & \multicolumn{2}{c}{**Designation**} & & \\
**Biopeptide Sequence** & **Mass** & **Allocated to the Sequence in the Database (ID 1, 2 or References 3)** & **Biological Activity** & **Tested Strain of** _Lb. Inleleticus_ \\ \hline RPKPHIKHQGLPQ & 1534.88 & biopep01213 1 & antihypertensive & B734, DSMZ \\ \hline TQSLVYP & 806.42 & biopep01306 & antihypertensive & \\ \cline{2-4}  & & 3333/BIOPEP-UWM2 & ACE inhibitory & DSMZ \\ \hline SRY 4 & 424.21 & biopep01260 & antihypertensive & B734 \\ \hline GKEKV & 559.33 & biopep00360 & antihypertensive & B734, DSMZ \\ \hline ENLHLP & 721.37 & biopep00179 & antihypertensive & B734, DSMZ \\ \hline AQTQSL & 646.33 & biopep00067 & antihypertensive & B734 \\ \hline FAQTQS & 680.31 & biopep00212 & antihypertensive & B734 \\ \hline YPF & 522.25 & \begin{tabular}{c} biopep04755, \\ 2868/BIOPEP-UWM, \\ [33] \\ \end{tabular} & opioid & & \\ \cline{2-4}  & & [34] & anticancer & \\ \hline GLPQE & 542.27 & \begin{tabular}{c} 8163/BIOPEP-UWM \\ 9375/BIOPEP-UWM \\ \end{tabular} & \begin{tabular}{c} antibacterial \\ \cline{2-4}  & B734 \\ \end{tabular} \\ \hline VQVTSTAV & 803.44 & \begin{tabular}{c} biopep01445 \\ \cline{2-4}  & 8264/BIOPEP-UWM \\ \end{tabular} & \begin{tabular}{c} antibacterial \\ \cline{2-4}  & B734 \\ \end{tabular} \\ \hline VPSERYL & 862.45 & \begin{tabular}{c} biopep01442 \\ \cline{2-4}  & 9250/BIOPEP-UWM, \\ [36] \\ \end{tabular} & ACE inhibitory & B734 \\ \hline INQF & 520.26 & biopep00551 & antihypertensive & B734 \\ \hline TVY 4 & 381.19 & \begin{tabular}{c} biopep01319 \\ [37] \\ \end{tabular} & ACE-inhibitory & B734 \\ \hline FPPQSVL & 786.43 & biopep00270 & antihypertensive & B734 \\ \hline LQPEVMGVSK & 1086.57 & biopep00867 & antihypertensive & DSMZ \\ \hline AYPS & 436.20 & \begin{tabular}{c} 8472/BIOPEP-UWM \\ \end{tabular} & \begin{tabular}{c} antioxidative \\ \cline{2-4}  & B734, DSMZ \\ \end{tabular} \\ \hline VRSP & 457.26 & \begin{tabular}{c} biopep01460 \\ \cline{2-4}  & 8309/BIOPEP-UWM \\ \end{tabular} & \begin{tabular}{c} ACE inhibitory \\ \end{tabular} & B734, DSMZ \\ \hline LVYPFPGPI & 1001.56 & \begin{tabular}{c} biopep00927 \\ [38] \\ \end{tabular} & \begin{tabular}{c} antihypertensive \\ \cline{2-4}  & B734, DSMZ \\ \end{tabular} \\ \hline NENLLRFFVAPFFPE & 1691.87 & biopep00991 & antihypertensive & B734 \\ \hline LQPEVMG & 772.38 & biopep00866 & antihypertensive & DSMZ \\ \hline FVAPFFEVFGKEKVNE & 1835.94 & biopep00305 & antihypertensive & B734 \\ \hline LEQL & 501.28 & biopep00755 & antihypertensive & B734, DSMZ \\ \hline ENLLRF & 790.43 & \
\begin{tabular}{c} biopep00182 \\ [39] \\ \end{tabular} &
\begin{tabular}{c} antihypertensive \\ \cline{2-4}  & B734 \\ \end{tabular} \\ \hline FPPQS & 574.27 & 9378/BIOPEP-UWM & ACE inhibitory & DSMZ \\ \hline FVAPFFEVFGK & 1236.65 & biopep00304 & antihypertensive & B734, DSMZ \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Sequences of biopeptides detected in the hydrolysates of the tested whey protein preparation. The most widespread bioactivity among all of the detected biopeptides sequences with molecular mass in the range of 300-3000 Da (Tables 3 and 4) was antihypertensive. It was also observed that some of BAPs sequences were generated only by one strain (DSMZ or B734). Moreover, thirty different biopeptides were released in the process of WPI hydrolysis by B734, while twenty BAPs were obtained by application of the reference strain (Table 4). In addition, the use of _Lb. helveticus_ B734 to hydrolyze WPI yielded sequences of immuno- and cyto-modulatory (LLY) and opioid (YPFP) peptides, which were absent in hydrolyses obtained with application of strain DSMZ 20075.