## 3 Results
### Characterization of Nutritional Quality  
We evaluated the amino acid composition and the content (g/100 g) in each batch of octopus, as shown in Table 2. Glutamic acid (2.25-2.33 g/100 g, leeward vs. windward) was found to be the major component within muscle proteins, followed by ornithine and  
\begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Common Octopus** & \multicolumn{2}{c}{**Windward**} & \multicolumn{2}{c}{**Leeward**} \\ \hline
**Nutritional Parameters** & **Average** & **SEM** & **Average** & **SEM** \\
**(g/100 g)** & & & & \\ \hline Protein & 17.75 & 0.55 & 16.23 & 1.08 \\ Na & 0.35 & 0.01 & 0.51 & 0.02 \\ Salt & 0.875 & 0.01 & 1.19 & 0.07 \\ Moisture & 79.5 & 0.65 & 81.25 & 0.85 \\ Ash & 1.88 & 0.09 & 2.04 & 0.08 \\ Carbohydrates & \textless{}2 & - & \textless{}2 & - \\ Total fats & \textless{}1 & - & \textless{}1 & - \\ Energetic value (Kcal/100 g) & 75.5 & 2.60 & 64.73 & 2.47 \\ \hline \hline \end{tabular}
\end{table}
Table 1: Nutritional composition of windward and leeward common octopus (_Octopus vulgaris_).  
taurine, aspartic, arginine, leucine, and lysine. Typtophan was not analyzed. No significant statistical differences were found based on the octopus origin.  
Figure 1 illustrates the amino acid composition of octopus essential amino acids (EAA), those that our organism cannot synthesize, and the non-essential amino acids (NEAA) that humans can produce. In both cases, the percentage of essential amino acids is high, near 40%.  
Other nutritional quality indicators, such as FR and PER, were estimated. The values obtained were very high in both batches of octopus, as shown in Table 3.  
Figure 1: Percentage of essential (EAA) vs. non-essential (NEEA) amino acids in octopus, both windward and leeward. Average of three samples for each origin.  
\begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Octopus vulgaris** & \multicolumn{2}{c}{**Windward**} & \multicolumn{2}{c}{**Leeward**} \\ \hline
**Amino acids (g/100 g)** & **Average** & **SEM** & **Average** & **SEM** \\ \hline Cysteine & 0.025 & 0 & 0.032 & \\ Methionine & 0.28 & 0.09 & 0.24 & 0.03 \\ Alanine & 0.45 & 0.16 & 0.75 & 0.18 \\ Arginine & 1.08 & 0.4 & 1.7 & 0.16 \\ Aspartic acid & 0.99 & 0.38 & 1.6 & 0.32 \\ Glutamic acid & 2.33 & 0.34 & 2.25 & 0.31 \\ Glycine & 1.07 & 0.14 & 0.94 & 0.88 \\ Histidine & 0.5 & 0.06 & 0.47 & 0.01 \\ Hydroxyproline & 0.26 & 0.06 & 0.15 & 0.03 \\ Isoleucine & 0.78 & 0.11 & 0.77 & 0.09 \\ Leucine & 1.28 & 0.22 & 1.25 & 0.2 \\ Lysine & 1.23 & 0.22 & 1.19 & 0.19 \\ Ornithineine/Taurine & 1.5 & 0.2 & 1.39 & 0.06 \\ Phenylalanine & 0.58 & 0.04 & 0.53 & 0.02 \\ Proline & 0.76 & 0.12 & 0.70 & 0.07 \\ Serine & 0.79 & 0.15 & 0.71 & 0.10 \\ Threonine & 0.97 & 0.17 & 0.95 & 0.11 \\ Tyrosine & 0.45 & 0.04 & 0.43 & 0.03 \\ Valine & 0.75 & 0.07 & 0.75 & 0.06 \\ \hline \hline \end{tabular}
\end{table}
Table 2: Composition and amount of amino acids (g/100 g) analyzed in windward and leeward common octopus (_Octopus vulgaris_). Average \(\pm\) SEM (\(n=3\)).  
To provide more nutrition information, the octopus amino acid score was estimated by comparing it with the recommended amino acid score.  
Results show that octopus contain all essential amino acids (EAAs) required to meet the recommended amino acid concentrations per unit protein for children older than three years, adolescents, and adults (aa score > 100%), Figure 2A,B. However, results show that it seems to be deficient in methionine and cysteine (aa score 75%) (Figure 2B). Nevertheless, it is known that cys is highly unstable; it forms oxidized compounds or is lost in acid hydrolysis, so with this method, the concentration tends to be underestimated. Work is being done to optimize the method for a more accurate estimation.