## 2 Materials and Methods
### Measurement and Analyses of Cannabinoid Content  
The cannabinoids in female hemp were analyzed by modifying the method described by [PERSON] et al. [43]. Briefly, the aboveground parts of the nine-week-old female hemp plants were separated into leaves and infloresances and dried in a freeze dryer (TFD550, Ishin BioBase Co. Ltd., Dongducheon, Korea) for seven days. The dried samples were ground into powder using a mortar and pestle. The powder (100 mg) was placed into a 2.0 mL tube and mixed with a solution of MeOH: hexane (9:1; 2 mL) by vortexing for 1 min, followed by sonication (powersonic420, Hwashin Tech Co. Ltd., Daegu, Korea) at 25 \({}^{\circ}\)C for 20 min to extract the cannabinoids. After sonication, the mixture was vortexed for 1 min and centrifuged at 13,000 rpm for 5 min to extract the supernatant. The extracted supernatant was filtered through a 0.45 \(\upmu\)m syringe filter (25 HP020 AN, Advantech Co. Ltd., Asan, Korea) (1 mL per sample) and transferred to high-performance liquid chromatography (HPLC) vials for analysis. The HPLC system consisted of an Agilent 1260 Infinity II binary pump (G7112B, Agilent Technologies Inc., Santa Clara, CA, USA), Agilent 1260 Infinity II Vialsampler (G7129C Agilent Technologies Inc., Santa Clara, CA, USA), Agilent 1260 Infinity II multicolumn thermostat (G7116A, Agilent Technologies Inc., Santa Clara, CA, USA) and Agilent 1260 Infinity II diode array detector HS (G7117C, Agilent Technologies Inc., Santa Clara, CA, USA). Chromatographic separation was achieved using a Poroshell 120 EC-C18 column (4.6 \(\times\) 50 mm, 2.7 \(\upmu\)m, Agilent Technologies Inc., Santa Clara, CA, USA) and gradient elution with 0.1% formic acid (reagent grade, \(>\)96%, Sigma-Aldrich, Saint Louis, MO, USA) in water (HPLC grade, Ducsan Pure Chemical Co. Ltd., Incheon, Korea) as mobile phase A, and 0.1% formic acid in acetonitrile (HPLC grade, Ducsan pure chemical Co. Ltd., Incheon, Korea) as mobile phase B. The flow rate and oven temperature were 1 mL\(\cdot\)min\({}^{-1}\) and 25 \({}^{\circ}\)C, respectively and the gradient conditions were as follows: 0-5 min, held at 55% B; 5-25 min, increased to 85% B; 25-30 min, held at 85% B; 30-30.1 min, decreased to 55% B; 30.1-35 min, held at 55% B. The injection volume was 10 \(\upmu\)L, and full spectra were recorded from 200 to 800 nm. For quantification, the detection wavelength was set at 210 nm.  
The cannabinoid standards for CBDA (CAS No. 1244-58-2) and CBD (CAS No. 13956-29-1) and THCA (CAS No. 23978-85-0) were acquired from Lippond (Arleisheim, Switzerland). \(\uplambda\)9-THC (CAS No. 1972-08-3) was purchased from Sigma-Aldrich (Saint Louis, MO, USA). These were used to determine the retention times of each cannabinoid and prepare a six-point calibration curve. A calibration verification standard was injected at the start of each analysis day to verify retention times and quantitation. Quantification was performed using calibration curves ranging from 50 to 1,000 \(\upmu\)g\(\cdot\)mL\({}^{-1}\). The linear equations were y = 32.251x + 19.131 for CBDA, y = 83.907x \(-\) 24.125 for CBD, y = 31.180x + 35.423 for THCA and y = 86.082x \(-\) 0.954. The experiment was performed three times.