_Applied_  
_sciences_  
_Article_  
**Effectiveness of Hyaluronan Autocross-Linked-Based Gel in the Prevention of Peritendinous Adherence Following Tencolysis**  
**[PERSON] 1,\({}^{\dagger}\), [PERSON] 1,\({}^{\ast}\),\({}^{\circledR}\), [PERSON] 1, [PERSON] 1\({}^{\circledR}\), [PERSON] 1, [PERSON] 2, [PERSON] 3\({}^{\circledR}\), [PERSON] 4 and [PERSON] 1**  
1 Department of Plastic and Reconstructive Surgery-Hand Surgery Unit, Azienda 'Ospedali Riunti', 60126 Ancona, Italy; [EMAIL_ADDRESS] (A.M.); [EMAIL_ADDRESS] (P.P. ); [PERSON]_ADDRESS] (L.S. ); [EMAIL_ADDRESS] (A.C.); [PERSON]] (M.R.) 2 Veterinary Medical School, University of Camerino, 62024 Camerino, Italy; [EMAIL_ADDRESS]
3 Department of Clinical and Biological Sciences & Neuroscience Institute of the Cavalieri Ottonghia Foundation, University of Turin, 10124 Turin, Italy; [EMAIL_ADDRESS]
4 Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, 44121 Ferrara, Italy; [EMAIL_ADDRESS]
* Correspondence: [EMAIL_ADDRESS]; Tel. : +39-071-5963945; Fax: +39-071-5965297
* The authors contributed equally to the work. **Featured Application: Local application of Hyaloglide@avoids adherence formation after tenolysis and supports normal restoring with regeneration of the synovial sheath in a rabbit model. **  
**Citation:** [PERSON], [PERSON]; [PERSON], [PERSON]; [PERSON]; [PERSON] [PERSON]; [PERSON] [PERSON]; [PERSON], [PERSON]; [PERSON] [PERSON]; [PERSON] [PERSON]; [PERSON], [PERSON] Effectiveness of Hyaluronan Autocross-Linked-Based Gel in the Prevention of Peritendinous Adherence Following Tencolysis. _Appl. Sci._**2021**, 7613. [[https://doi.org/10.3390/appl1167613](https://doi.org/10.3390/appl1167613)]([https://doi.org/10.3390/appl1167613](https://doi.org/10.3390/appl1167613))  
**Publicher's Note:** MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. **Copright:** 2021 by the authors. Licence MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([[https://creativecommons.org/licenses/by/4.0/](https://creativecommons.org/licenses/by/4.0/)]([https://creativecommons.org/licenses/by/4.0/](https://creativecommons.org/licenses/by/4.0/))). **Abstract:** Peritendinous adhesions are a frequent occurrence following tenolysis and present a major clinical challenge regarding prevention and management, with no recovery assured from conservative or surgical approaches. Herein, we investigated the effectiveness of Hyaloglide\({}^{\circledR}\), a hyaluron gel-based product with a novel autocross-linked technology, in a rabbit model affected by tenolysis on the flexor digitorumum communis tendon (FDC). A 1.5-cm-long scrubbing of the tendon surface was performed bilaterally to induce periodontitis adhesion on FDC of 30 animals with subsequent application of Hyaloglide\({}^{\circledR}\) on the surrounding injured area, in one randomly chosen tendon. The contralateral tendon was treated with saline solution as the control. We sacrificed the rabbit models after 45 days of surgery and quantitatively assessed the generation of peritendinous adherence and regeneration of the tendon sheaths using histological (hematossyline-eosine, masson's trichromic), histomorphometrical (Tang score, Soslowsky Svenson, and Cook score), light electron microscopic, and gene expression investigations. Four rabbits were devoted to biomechanical analysis. Peritendinous adhesions were limited in Hyaloglide\({}^{\circledR}\)-treated tendons; moreover, well-regenerated tendon sheaths were observed conversely to untreated tendons presenting with extensive areas of adhesions on the tendon surface. Histomorphometrical analysis revealed an adhesion score (Tang score) significantly better in the treated group (\(p\) = 0.001 *) compared to the control group.