## 3 Results and Discussion
### The Effect of Fruit Enzymes at Differential Concentration on the Change of Various Floaters
As of 2011, PPV may be a better option for more diffuse vitreous opacities, fibril strands, VMT and FTMHs and could remove the damaged vitreous, thereby clearing the floaters in the visual axis. Although the results showed that 85% of participants were satisfied by surgery, most of the patients preferred food supplements to avoid the higher number of side effects from surgery and desired the safe and more effective conservative treatment to be developed through future research. Except for observation, laser and PPV, the pharmacologic vitreolysis developed recently, intravitreal ocriplasmin (microplasmin) (Jetrea; Thrombogenics USA, Alcon) injection is a recombinant and truncated form of human 27 kilodalton serine protease plasmin, a non- surgical option and alternative treatment instead of PPV for VTM that induces smaller fragments of enzyme [25; 26]. Furthermore, ocriplasmin is enzymatically able to degrade cleaved collagens, fibronectin, laminin, over growth tissues, extra-ECM, futile vitreous fibrils and the protein scaffold between the vitreo-retinal space. For patients with SOVs, a single intravitreal injection of 0.125 mg should be used to treat the subjects by cutting vitreous strands and opacity. Current options showed that ocular floaters, SVOs and proliferative tissues could be dissolved by the proteolytic and colleagonic functions of ocriplasmin. Moreover, the mechanism of ocriplasmin for treating SVOs and enhance the traction release successfully and achieved an improvement of 26.5% at Day 28 [27]. Ocular floaters and SVOs are the consequence of vitreous attachment with disturbance of the retina, which would be dissolved by ocriplasmin. It was even reported that patients with SVOs who received an ocriplasmin injection had a response rate that reached about 11%. In addition, dose- and time-dependent cleavage was achieved by ocriplasmin between posterior vitreous cortex and ILM. However, the adverse events included acute reduction in vision, metamorphosis, visual field defect, ERG changes, dyschromatopsia, retinal tear or detachment,lens subluxation and phacodonesis. Intravitreal injection also has several complications such as endophthalmitis, glaucoma, cataract and even RD [25]. During aging, the vitreous progressively liquefies and pockets of liquid form in the gel. The first liquefaction has been observed in individuals at 4 year of ages when 20% of the vitreous is liquid; after age 40 liquefaction increases so that by age 80, more than half of the vitreous is liquid which is called \"synchysis\" [26]. This age-related redistribution of the fibrillar components of the vitreous leaves the spaces between the collagen cables filled with liquid. The process has been termed \"synchysis senile\". The collagen fibrils of the core aggregate into thick cables, instead of the random orientation of fine fibrils in the gel network earlier in life. Liquefaction is caused by the aggregation of collagen fibrils which induces redistribution of the fibrils, with the aggregates becoming more highly concentrated in some of the gel, while other parts of the vitreous developed a lack of fibrils and are converted into liquid compartments. Over the next 1 to 3 months, the vitreous gel further condenses, and the sides of the gel also separate from the retina until the PVD is complete [27]. Hence, the shadows cast on the retina could reveal ocular floaters. The collagen filaments aggregation and condensation resulted in the formation of larger fibrils, which float in lacunas of liquefied vitreous giving the patients the perception of floaters. The speed at which these vitreous changes happen depends on age, environmental factors, exposure to sunlight or blue light, oxidative stress and HA-collagen interaction [28]. If PVD gently progresses, the symptoms are mild. However, if the forces of separation are strong, PVD could tear the retina or retinal blood vessels. Moreover, in PVD, the vitreous shrinks and leads to ocular floaters.