## Materials and Methods
### Lab Incubation and Greenhouse Experiment  
After pre-incubation, the amended soils were transported to the University of Wyoming College of Agriculture and Natural Resources Research and Extension Center Greenhouse Complex in Laramie, Wyoming. The temperature in the greenhouse was set to 21.1 \({}^{\circ}\)C and 18.3 \({}^{\circ}\)C during the day and night, respectively, with no supplemental lighting. Sixty-four plastic cone-tainers were filled with approximately 670 cubic centimeters of soil and planted with two varieties of vegetable seeds: a leafy green, _Lactuca sativa_ L. (lettuce), and a root vegetable, _Raphanus sativus_ L. (radish). The lettuce (\"Emerald Jewel\" cultivar) was obtained from Burpee in Warminster, Pennsylvania, and theadish (Rover F1 cultivar) was obtained from Johnny's Selected Seeds in Winslow, Maine. Both were kindly provided by Dr. [PERSON] (University of Wyoming, Laramie, Wyoming). The plants were chosen due to their commonality among local home gardeners and as representative species for above and belowground biomass, respectively. The cone-tainers had limited perforation at the bottom, allowing for leaching of the soil solution with minimal loss of treated soil. To increase the likelihood of germination for each replicate, cone-tainers planted with lettuce were seeded with four seeds and those with radish with two seeds. All cone-tainers were thinned to one plant per cone-tainer within a week of seedling emergence, except for those in which germination did not occur. The cone-tainers were arranged in an 8 cone-tainer \(\times\) 8 cone-tainer square using a completely randomized design. One additional layer of cone-tainers planted with radish were also placed on the outside of the experiment perimeter to act as an edge row and help reduce potential moisture loss. Soil moisture was monitored and adjusted every one-to-two days to reach 20-30% (_w/w_). This was achieved by comparing the weight of a cone-tainer at the ideal moisture content (\"par weight\") for the treatment type against the average weight (g) of two reference cone-tainers for each treatment and plant species combination. The average reference weight was subtracted from the ideal par weight to estimate moisture loss, then an equivalent amount of tap water was added as needed to each cone-tainer to bring it back up to the par weight for its species and treatment type. The experiment was terminated, and plants were harvested after 12 weeks in the greenhouse. \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Chemical Composition** & **Biochar** & **Compost** & **Inorganic Fertilizer** \\ \hline
**Carbon and Nitrogen:** & & \(-\%\)\(\%\) & \\ Total Nitrogen & 0.42 & 1.24 & n/a \\ Total Carbon & 65.10 & 8.57 & n/a \\ C-to-N ratio & 154 & 7 & n/a \\
**Macronutrients:** & & \(-\%\)\(\%\) & \\ Organic N & n/a & 1.15 & n/a \\ Ammonium & n/a & 0.004 & 3.500 \\ Nitrate & n/a & 0.086 & 20.50 \\ Available Phosphorus & 0.11 & 0.85 & 8.00 \\ Potassium & n/a & 2.13 & 16.00 \\ Calcium & 3.21 & 3.93 & n/a \\ Magnesium & 0.39 & 0.68 & n/a \\ Sulfur & 0.06 & 0.41 & n/a \\
**Micronutrients:** & & \(-\)ppm– & \(\%\)\(\%\) \\ Boron & 29.2 & 28.4 & 0.02 \\ Copper & n/a & 23.3 & 0.07 \\ Iron & 181 & 8933 & 0.15 \\ Manganese & 51 & 241.6 & 0.05 \\ Molybdenum & 0.07 & n/a & 0.0005 \\ Zinc & 53 & 95.7 & 0.06 \\
**Trace Elements:** & & \(-\%\)\(\%\) & \\ Sodium & n/a & 0.33 & n/a \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Chemical properties of amendments prior to addition of native soil and water. The mineral composition of the inorganic fertilizer was sourced from the product label. Results for biochar and composted manure were obtained through laboratory testing. Use of “n/a” in the table indicates that a specific element was not provided with the laboratory report or product label but does not necessarily suggest that the element is entirely absent. Concurrent to the greenhouse experiment, a portion of the treated soils (\"Lab Inc\") continued to be incubated in the lab.