# Molecularly-Imprinted SERS: A Potential Method for Bioanalysis
## 4 Molecularly-Imprinted SERS Methods
### One Step MIP-SERS  
[PERSON], et.al, developed benzimidazole analysis using the single-step MIP-SERS method. The type used is the core-shell formation type. Ag microspheres were used as the core, and then coated with MIP. MIP in this study was synthesized using a dummy template, namely carbendazim. The use of carbendazim as a dummy template and reducing background noise can also avoid the phenomenon of template leakage in MIP making. Based on the validation results of the analytical method, this method was proven to be used for qualitative and semi-quantitative analysis of benzimidazole on samples with complex matrix [28]. Another study developed an analytical method for detecting Rhodamine 6G using SiO\({}_{2}\)/Ag/MIP nanocomposites. SiO\({}_{2}\) is used as a buffer that will adsorb Ag+ ions, which are then reduced with ethanolamine. In this study, SiO\({}_{2}\)/Ag, which acts as a substrate and acts as a core, was then covered with an MIP using a surface molecularly imprinting technology (SMIT) method. The combination of SiO\({}_{2}\)/Ag with a MIP is expected to overcome the shortcomings of MIP in the form of low binding capacity and low bond kinetics. MIP was synthesized by the precipitation polymerization method. The thickness of the MIP layer on the SiO\({}_{2}\)/Ag surface is regulated by adjusting the number of crosslinkers in the pre-polymerization reaction. The thickness of this layer will affect the detection with SERS. The ratio of monomer and crosslinker is 1:3, giving the maximum MIP layer thickness for SERS detection, which is 40 nm. The ratio increases, the SERS signal decreases [49]. [PERSON] et al., also developed a nanocomposite between AgNPs and MIP (AgNPs@MIP) for the detection of caffeine residues in wastewater. This study used a dummy template, theophylline, which is similar to caffeine. In this study, AgNPs were spread on MIP, then the formed nanocomposite was used as an adsorbent for SPE cartridges. The one step MIP-SERS method of this type can reduce the shortcomings of the one step MIP SERS method with the core shell type which requires synthesis conditions that are difficult to control and reproduce. This method can detect caffeine in river water samples with a fast analysis time of 23 min. The same mechanism was used for the analysis of bisphenol A on polycarbonate plastic samples. AgNPs substrates are formed in situ in MIP. The AgNPs formed are expected to be evenly distributed to support the analyte-AgNPs interaction and increase the hotspot effect, which can increase the signal at the time of SERS detection [50]. \begin{table}
\begin{tabular}{l l l l l l l l l l l l} \hline \hline \multirow{2}{*}{**No**} & \multicolumn{2}{c}{**Cineineineologicalological**} & \multirow{2}{*}{**Samples**} & \multicolumn{2}{c}{**Methods**} & \multicolumn{2}{c}{**Functional**} & \multicolumn{2}{c}{**Template**} & \multicolumn{2}{c}{**Koolistic**} & \multicolumn{2}{c}{**Assolved Performance**} & \multicolumn{2}{c}{**Ref. **} \\ \cline{2-11}  & \multicolumn{2}{c}{**Compounds**} & \multicolumn{1}{c}{**Compounds**} & & & & & & & & & & \\ \hline
16. & Typune & Aqueous medium & FDA MIP SBS & Ag & AM & Typune & EDCAA & ND & LOD \(\cdot 10^{-14}\) med/L & [129] \\ \hline
17. & p-circumulative & Water & DC/Ag-MIP SBS & Ag & Methacrylamide & p-circumulative & \multicolumn{2}{c}{P-circumulative} & \multicolumn{2}{c}{N-N, [PERSON] et al., developed an analytical method for 2,6-dichlorophenol using SiO\({}_{2}\)/rGO/Au composites, SGA, as SERS substrate. The composites made are expected to increase the sensitivity of the SERS substrate. The combination of composites with MIP is further expected to increase the selectivity of the SERS substrate. Through the SMIT (surface molecularly imprinting technology) mechanism, it is hoped that specific cavities that recognize certain compounds can freeze on the surface of the SERS substrate.