## 4 Data  
Datasets publicly available can be broadly subdivided into those devoted solely to segmentation (see Table 2), event detection and classification (and eventually also tracking, see Table 3), or tracking (and eventually also segmentation, see Table 4). Observe that the numbers reported in these tables refer solely to traditional label-free images/image sequences, which is the focus of this review; nonetheless, many of the reported datasets also have data from other microscopy types. The reported numbers specify only images for which annotations exist (there could be other images but without annotations). \begin{table}
\begin{tabular}{l c c c c c c} \hline \hline
**Name** & **[Ref]** & **Content** & **Link** & **\# Imgs** & **\# Cells** & \begin{tabular}{c} **\# Cell** \\ **Lines** \\ \end{tabular} \\ \hline Allen Cell Explorer & [86] & \
\begin{tabular}{c} 3D Label- \\ Free \\ onLabelfreeTrainingData \\ \end{tabular} & [[https://www.cs.bu.edu/fac/betke/BiomedicalImageSeg](https://www.cs.bu.edu/fac/betke/BiomedicalImageSeg)]([https://www.cs.bu.edu/fac/betke/BiomedicalImageSeg](https://www.cs.bu.edu/fac/betke/BiomedicalImageSeg)) & 151 & 151 & 3 \\ BU-BIL & [87] & PhC & [[https://www.cs.bu.edu/fac/betke/BiomedicalImageSeg](https://www.cs.bu.edu/fac/betke/BiomedicalImageSeg)]([https://www.cs.bu.edu/fac/betke/BiomedicalImageSeg](https://www.cs.bu.edu/fac/betke/BiomedicalImageSeg)) & 151 & 151 & 3 \\  & & & & & \\ CTC & [22] & PhC, DIC, BF & [[http://www.celltrackingchallenge.net](http://www.celltrackingchallenge.net)]([http://www.celltrackingchallenge.net](http://www.celltrackingchallenge.net)) & 213 & 1980 & 5 \\ DeepCell & [23] & PhC & [[https://doi.org/10.1371/journal.pcbi.1005177.s021](https://doi.org/10.1371/journal.pcbi.1005177.s021)]([https://doi.org/10.1371/journal.pcbi.1005177.s021](https://doi.org/10.1371/journal.pcbi.1005177.s021)), https: & 45 & \(\sim\)4300 & 1 \\  & & & & & \\ EVICAN & [88] & PhC, BF & [[https://edmond.mpdl.mpg.de/dataset.shtml?persistentId=](https://edmond.mpdl.mpg.de/dataset.shtml?persistentId=)]([https://edmond.mpdl.mpg.de/dataset.shtml?persistentId=](https://edmond.mpdl.mpg.de/dataset.shtml?persistentId=)) & 4640 & 26,428 & 30 \\  & & & & & & \\ fastER & [25] & PhC, BF & [[https://bsse.ethz.ch/csd/software/faster.html](https://bsse.ethz.ch/csd/software/faster.html)]([https://bsse.ethz.ch/csd/software/faster.html](https://bsse.ethz.ch/csd/software/faster.html)) & 39 &
\begin{tabular}{c} 1653 \\ (+953) 1 \\ \end{tabular} & 2 \\ LIVEcell & [26] & PhC & [[https://sartorius-research.github.io/LIVECell/](https://sartorius-research.github.io/LIVECell/)]([https://sartorius-research.github.io/LIVECell/](https://sartorius-research.github.io/LIVECell/)) & 5239 & 1,686,352 & 8 \\ [PERSON] & [63] & PhC & [[https://github.com/ElsevierSoftwareX/SOFTX_2018_158](https://github.com/ElsevierSoftwareX/SOFTX_2018_158)]([https://github.com/ElsevierSoftwareX/SOFTX_2018_158](https://github.com/ElsevierSoftwareX/SOFTX_2018_158)) & 37 & 2641 & 1 \\ [PERSON] et al. & [20] & PhC, DIC, HMC & [[https://zenodo.org/record/1250729](https://zenodo.org/record/1250729)]([https://zenodo.org/record/1250729](https://zenodo.org/record/1250729)) & 32 & 4546 & 1 \\ \hline \hline \end{tabular}  
* For other 953 cells, only centroids are provided.  
\end{table} 
Table 2: Details of available annotated data for cell segmentation in traditional label-free images: dataset name (Name); reference ([Ref]); type of microscopy data (Content); url (Link); number of annotated images (# imgs), annotated cells (# cells), and cell lines (#cell lines). All links were accessed on 28 August 2022. **Allen Cell Explorer**[86] includes a massive collection of light microscopy cell images with manually curated segmentation masks for 12 cellular components, as reported in [88]. **BU-BIL** (Boston University-Biomedical Image Library) [87] includes six datasets, three of which consisting of PhC images from different cell lines.