## 5 Conclusions  
In summary, the present study describes _Streptomyces_ sp. CH6 isolated from the soil in Vietnam as a novel species with promising antibacterial, antioxidant, and anticancer properties. The crude extract of _Streptomyces_ sp. CH6 showed broad-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria, as well as antioxidant and selective anticancer properties in vitro. The genome of _Streptomyces_ sp. CH6 consists of a 6,936,977 bp chromosome with a GC content of 73% and encodes 5831 proteins. Analysis revealed 13 putative biosynthetic gene clusters (BGCs) showing similarity (13.2-80.7%) to those responsible for the production of known antibacterial, antioxidant, and antitumor compounds. These findings suggest that _Streptomyces_ sp. CH6 has the genetic potential to produce novel secondary metabolites. Overall, this study provides a comprehensive genomic overview of _Streptomyces_ sp. CH6 and lays the groundwork for future experimental validation of guangnanmycin production or the discovery of other bioactive compounds with potential medical applications.  
The following supporting information can be downloaded at: [[https://www.mdpi.com/article/10.3390/app15137126/s1](https://www.mdpi.com/article/10.3390/app15137126/s1)]([https://www.mdpi.com/article/10.3390/app15137126/s1](https://www.mdpi.com/article/10.3390/app15137126/s1)), Figure S1: Antifungal activity against _Col_letotrichum gloeosporoides_ (**A**) and _Fusarium solani_ (**B**) of CH6; Figure S2: Colony morphology (**A**) and hyphal growth observed via light microscopy at 40\(\times\) (**B**); Figure S3: Prediction of biosynthetic gene clusters (BGCs) present in _Streptomyces_ sp. CH6. Genes are color-coded in antiSMASH by predicted function, as indicated in the bottom legends; Figure S4: Multiple sequence alignments of GnmP (**A**) and GnmY (**B**) from _Streptomyces_ sp. CH6 with their homologs from _Streptomyces_ sp. CB01883. GnmP and GnmY from the CH6 strain shared 87.5% and 84.9% sequence identity with those from the CB01883 strain, respectively. The multiple sequence alignment was performed using Clustal Omega ([[https://www.ebi.ac.uk/jdispatcher/msa/clustalo](https://www.ebi.ac.uk/jdispatcher/msa/clustalo)]([https://www.ebi.ac.uk/jdispatcher/msa/clustalo](https://www.ebi.ac.uk/jdispatcher/msa/clustalo)), accessed on 21 May 2025) and presented using Jalview ([[https://www.jalview.org/](https://www.jalview.org/)]([https://www.jalview.org/](https://www.jalview.org/))). Intensity of the blue color gradient is based on 50% identity; Table S1: Morphological, physiological, and biochemical characteristics of CH6 strain.  
Conceptualization, C.T.N. and T.T.T.T.; methodology, C.T.N., H.T.N., V.T.H.D., K.P.D. and T.T.T.T.; investigation, C.T.N. and H.T.N.; validation, C.T.N., H.T.N. and V.T.H.D.; formal analysis, C.T.N., T.T.T.T. and K.P.D.; writing--original draft preparation, C.T.N. and H.T.N.; writing--review and editing, T.T.T.T. All authors have read and agreed to the published version of the manuscript.  
This research was funded by a grant from the Ministry of Education and Training, Vietnam, project number: B2023-MHN-02.  
Not applicable.  
The original contributions presented in this study are included in the article and Supplementary Materials. Further inquiries can be directed to the corresponding author.  
The authors declare no conflicts of interest.