## 3 Results
### Identification of S. aureus via Mass Peptide Analysis  
MBT's Compass software was used to analyze the recovered microorganisms in the ongoing experiment, and the acquired spectra were evaluated by comparing them to those in the MBT database. MBT's Compass software displayed a standard evaluation of many _S. aureus_ strains acquired from inpatient hospital specimens. According to our findings, up to 20 distended ion peaks were found in the existing bands from the region, ranging from three hundred to eleven thousand Daltons (Da) (Figure 1). Strong peaks were found between 3000 and 8000 Da (Figure 2). MRSA isolates from the field were matched with two MRSA benchmark strains, including _S. aureus_ DSM 3463 and _S. aureus_ DSM 20232, whereas MSSA isolates were matched with five MSSA reference strains, including _S. aureus_ ATCC 29213, _S. aureus_ ATCC 25923, _S. aureus_ DSM 20231, _S. aureus_ DSM 346, and _S. aureus_ DSM 799. In the present investigation, 26/26 (100%) MSSA isolates and 22/22 (100%) MRSA isolates were successfully identified, with a logarithmic score ranging between 2.300 and 3.000 for 15 MSSA isolates and 10 MRSA isolates, and a logarithmic value ranging from 2.00 to 2.29 for 10 MSSA isolates and 12 MRSA isolates. Interestingly, one MSSA was found to have a score ranging from 1.7 to 1.99. MSSA and MRSA isolates were identified by comparing their profiles to those in the MBT device dataset, which includes over 290 strains from 16 species from the ATCC and the DSMZ reference strains. The single-peak screening of several mass regions revealed regular divergences that might be used to classify MSSA and MRSA strains. We discovered a plethora of single-peak values in the region of 2770 to 6310 Da, revealing a wide variety of intensities between both the reported MSSA and MRSA strains. As a result, the single peak is a useful tool in differentiating MRSA from MSSA. The precise discoveries were constantly expanded into the areas of 2771 Da, 2996 Da, 3720 Da, 4480 Da, 4540 Da, and 6310 Da. In such circumstances, there were several differences in the strengths of the single peaks of concentration between MRSA and MSSA. MSSA (green color) had significant strength concentrations in the mass ranges of 2771 Da, 2996 Da, 3720 Da, 4480 Da, 4540 Da, and 6310 Da (Figure 3), whereas MRSA had none (red color). MRSA (red color) had higher intensity peaks in the mass ranges of 5530 Da, 6580 Da, 6710 Da, and 6820 Da (Figure 4) that were not seen in MSSA (green color). Furthermore, principal component analysis (PCA) is a supplementary mathematical technique taken from the MBT device's Compass program for evaluating statistical models to show the degree of likeness and diversity of diverse protein profile spectra. Similarly, as mentioned in the various mathematical evaluations, PCA decreases the variance of a complicated database. In three-dimensional (3d) PCA, several spectral proteins for MSSA and MRSA isolates were identified (Figure 5). Each spectrum is symbolized by a patch, and the varied colors show the mirrored subgroup contributions, with each dot representing the side view of one of the protein's parts of the spectrum. Figure 3: MSSA (green color) had single-peak intensities (2771 Da, 2996 Da, 3720 Da, 4480 Da, 4540 Da, and 6310 Da), whereas MRSA (red color) did not (red color). The preponderance of peaks, including all _S. aureus_ isolates, were tightly related and matched together, as shown by the cluster view of the 3d PCA (Figure 6). When it comes to PCA assessment groups, each peak may generate loading values produced from the PCs computation. During our experiment, each signal was assigned loadings of 1, 2, and 3, values derived from calculations of PC 1, 2, and 3. To illustrate the protein profiles for the 22 detected MRSA isolates, the Compass software programed in the MBT gadget provided an actual gel image. The spectra ranged from 3000 Da to 11,000 Da, with prominent peaks between 3000 and 8000 Da (Figure 7).