###### Abstract  
The primary objective of this study was to compare the nutrition and metabolite profiles of _Siraitia grosvenorii_ from different regions (namely Yongfu and Longsheng) and processing stages. Our findings showed that fresh _Siraitia grosvenorii_ from Longsheng contained higher levels of total sugars, protein, and crude fat compared with those from Yongfu, though both regions had similar dietary fiber and ash content. Dried Yongfu _Siraitia grosvenorii_ showed increased nutrient levels. A mineral analysis revealed that fresh _Siraitia grosvenorii_ from Yongfu had the highest levels of calcium, magnesium, and potassium along with distinct differences in other mineral concentrations compared with Longsheng. Notably, fresh Yongfu fruits had higher mineral content than dried ones, except for aluminum and selenium. Through an untargeted metabolomics analysis, we identified 470 metabolites, showing significant variation between fresh samples from Yongfu and Longsheng and between fresh and dried Yongfu samples. Key metabolites included carboxylic acids, fatty acyls, and organooxygen compounds. Additionally, we observed significant enrichment in metabolic pathways such as phenylpropanoid biosynthesis, galactose metabolism, and linoleic acid metabolism, with notable differences in metabolite regulation depending on the region and processing stage. These findings highlight the influence of regional environmental factors and drying processes on the nutrient and metabolite composition of _Siraitia grosvenorii_. S. Giraitia grosvenorii; nutrient composition; minerals; metabolites 21202025202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202020202202020202020202  
and separated a non-sugar substance with a sweetness equivalent to 150 times that of sucrose using column chromatography and thin-layer chromatography, suggesting it to be a triterpene glycoside. In the food and pharmaceutical industries, dried fruits of _S. grosvenorii_ are commonly used. This is conducted to facilitate storage and transportation, ensure the stability of the content of active ingredients, and make processing operations more convenient. However, the nutritional, mineral, and metabolite composition of _S. grosvenorii_ can vary significantly depending on factors such as the growing region and processing methods, which in turn impact its value and efficacy as a dietary component. Regional variations along with different processing stages influence the concentration of essential nutrients such as sugars, protein, and crude fat [4]. Minerals like calcium, magnesium, and potassium, crucial for physiological health [5], also differ by region, potentially due to environmental factors like soil composition and climate. Furthermore, processing methods, specifically drying, also play a significant role in altering the fruit's nutritional and mineral profiles, affecting its bioavailability and potential health impacts [6]. In addition to its nutrient and mineral content, _S. grosvenorii_ hosts a diverse array of metabolites, including carboxylic acids, fatty acyls, and organooxygen compounds [7]. Metabolomics, as an emerging scientific discipline, is dedicated to the qualitative and quantitative analysis of all low-molecular weight metabolites present within a biological organism or cell during a specific physiological stage [8]. In accordance with the distinct research objectives, metabolomics can be categorized into untargeted metabolomics and targeted metabolomics. Untargeted metabolomics undertakes the unbiased detection of all detectable metabolite molecules within a sample and subsequently conducts differential and pathway analyses via bioinformatics approaches to discern the disparities in metabolites between samples [9].