Effect of the Concentration of Extracellular Polymeric Substances (EPS) and Aeration Intensity on Waste Glycerol Vaporization by Docosahexaenoic Acid (DHA) Produced in Heterorophic Culture of _Schizochytrium_ sp  
[PERSON]  
1 InnovaTree Sp. z. o.o., 81-451 Gdynia, Poland; [EMAIL_ADDRESS] (N.K. ); [EMAIL_ADDRESS] (S.T. )1 InnovaTree Sp. z. o.o., 81-451 Gdynia, Poland; [EMAIL_ADDRESS] (N.K. ); [EMAIL_ADDRESS] (S.T. )1 InnovaTree Sp. z. o.o., 81-451 Gdynia, Poland; [EMAIL_ADDRESS] (N.K. ); [EMAIL_ADDRESS] (S.T. )1 InnovaTree Sp. z. o.o., 81-451 Gdynia, Poland; [EMAIL_ADDRESS] (N.K. ); [EMAIL_ADDRESS] (S.T. )1 InnovaTree Sp. z. o.o., 81-451 Gdynia, Poland; [EMAIL_ADDRESS] (N.K. ); [EMAIL_ADDRESS] (S.T. )1 InnovaTree Sp. z. o.o., 81-451 Gdynia, Poland; [EMAIL_ADDRESS] (N.K. ); [EMAIL_ADDRESS] (S.T. )1 InnovaTree Sp. z. o.o., 81-451 Gdynia, Poland; [EMAIL_ADDRESS] (N.K. ); [EMAIL_ADDRESS] (S.T. )1 InnovaTree Sp. z. o.o., 81-451 Gdynia, Poland; [EMAIL_ADDRESS] (N.K. ); szymon.talibierz@innovatree. tory conditions are not always consistent with respective values obtained from large-scale installations [12; 13]. Inexpensive carbon sources offering an alternative to glucose are also searched for. Given the fact that _Schizochytrium_ sp. biomass can grow on various carbon sources, including wastes, studies on the use of waste glycerol seem a justified prospective trend in scientific research [14]. So far, glycerol has been used as a carbon source in biochemical processes [15] and thermochemically converted to dipropylene glycol [16] and hydroxyacetone [17]. Other explored processes included its reformation to produce hydrogen and synthesis gas [18], esterification [19], hydrogenolysis [20], and production of epichlorohydrin [21]. Other ways of waste glycerol management for energetic purposes that have recently spurred great interest include production of ethanol (Saccharomyces cerevisiae) or \(\beta\)-carotene (Blakeslea trispora) [22; 23; 24]. Technological advance necessitates scale enlargement and verification of data obtained, that can be achieved via exploitation of pilot bioreactor installations followed by tests in the full technical scale [25; 26; 27; 28]. During culture scale enlargement, it is impossible to keep all technological parameters at the levels established and employed in the laboratory scale [29; 30]. Additionally, new difficulties and limitations often emerge, which determine the course and effectiveness of the whole process [31]. It is then indispensable to know the critical values of technological parameters having the greatest impact on the course of the entire incubation process [32]. One of the exploitation treatments posing severe difficulties in the large-scale facilities is the technologically and economically effective oxygen supply to bioreactors [33]. These difficulties stem from the cubature of facilities and growing viscosity of the culture medium during intensive production of _Schizochytrium_ sp. Biomass [34; 35]. Fluid viscosity affects the mixing process, mass transfer, and gas diffusion in the culture medium, and consequently, the Reynold number value, and correlates with the oxygen mass transfer coefficient [36; 37]. In high-density microbiological cultures with a high biomass concentration, the culture liquid turns viscous, which significantly influences hydrodynamics, heat and mass transfer, cell growth kinetics, and formation and accumulation of secondary metabolites in the culture medium [38; 39; 40]. It is believed that the increase in culture medium viscosity observed in the microorganism-mediated processes, including _Schizochytrium_ sp., is largely due to the production of extracellular polymeric substances (EPS) [41; 42]. Apart from technological difficulties and complications in establishing a commercial scale of high-density cultures, the viscosity increase caused by polysaccharide production may suppress lipid accumulation in cells and DHA production [43].