# De Novo Transcriptome Identifies Olfactory Genes in _Diachassimomorpha Ionigicaudata_ (Ashmead)
## 3 Results
### Differential Expression Analysis
\begin{table}
\begin{tabular}{l c c c c c} \hline \hline
**TranscriptID** & **G*** & **M*** & **C*** & **Protein/Gene** & **Description** \\ \hline
**OBP** & & & & & & \\ TRINITY\_DN22\_cd\_g3\_31 & 41,518.89 & 55,018.99 & 39,228.41 & OB56D & General odorant-binding protein 56d \\ cluster\_contig4670 & 20,459.11 & 27,957.99 & 83,228.40 & OB56H & General odorant-binding protein 56h \\ cluster\_contig378 & 15,552.04 & 13,752.58 & 20,997.13 & OB69A & General odorant-binding protein 69a \\ TRINITY\_DN641\_cd\_g2\_31 & 5,010.50 & 13,074.19 & 4,838.31 & OB69A & General odorant-binding protein 69a \\ TRINITY\_DN23528\_cd\_g1\_i & 3853.93 & 5367.95 & 6228.73 & OB56D & General odorant-binding protein 56d \\
**CSP** & & & & & \\ TRINITY\_DN1018\_cd\_g1\_i\_5 & 1105.74 & 15,620.31 & 10,036.05 & CSP & chemosensory protein \\ TRINITY\_DN661\_g1\_i\_1 & 9447.67 & 10,194.33 & 7724.67 & TrTix33221.1 & chemosensory protein 4 \\ TRINITY\_DN4258\_cd\_g1\_i\_i\_ & 4538.67 & 4738.33 & 5976.67 & AZQ24964.1 & chemosensory protein, partial \\ TRINITY\_DN188\_cd\_g1\_i\_56 & 5121.01 & 3247.27 & 4919.50 & CSP & chemosensory protein \\ cluster\_contig14748 & 3679.27 & 3944.22 & 2395.06 & TrTix33222.1 & chemosensory protein 5 \\
**OR** & & & & & \\ cluster\_contig3730 & 41,364.85 & 682,43.05 & 24,104.78 & LOC107043577 & Odorant receptor \\ TRINITY\_DN1357\_cd\_g1\_i\_5 & 18,042.97 & 23,565.93 & 11,831.46 & OR43A & Odorant receptor 43a \\ TRINITY\_DN1357\_cd\_g1\_i\_6 & 7765.79 & 27,969.19 & 13,421.25 & OR43A & Odorant receptor 43a \\ cluster\_contig12468 & 16,698.20 & 32,582.66 & 712.54 & LOC107043576 & Odorant receptor \\ cluster\_contig5799 & 2835.24 & 248.79 & 3479.03 & OR43A & Odorant receptor 43a \\
**GR** & & & & & \\ TRINITY\_DN9119\_cd\_g1\_i\_2 & 45.68 & 489.30 & 545.92 & GR107 & gustatory receptor Gr107 \\ TRINITY\_DN3525\_cd\_g1\_i\_10 & 288.94 & 333.98 & 230.17 & GR43A & 43-like \\ cluster\_contig36 & 290.10 & 43.82 & 30.95 & GR43a & gustatory receptor for sugar taste \\
**TRINITY\_DN14061\_cd\_g1\_3 & 91.79 & 116.05 & 78.74 & GR43a & 43-like \\ TRINITY\_DN4338\_cd\_g1\_i\_4 & 90.22 & 77.22 & 64.01 & GR107 & gustatory receptor Gr107 \\
**IR** & & & & & \\ TRINITY\_DN11328\_cd\_g1\_i\_1 & 41.09 & 39.27 & 45.12 & IR25A & Iontropic receptor 25a \\ cluster\_contig8505 & 39.77 & 34.65 & 39.58 & IR25A & Iontropic receptor 25a \\ TRINITY\_DN444\_cd\_g1\_i\_2 & 16.26 & 12.92 & 47.84 & IR25A & Iontropic receptor 25a \\ cluster\_contig11725 & 19.21 & 25.57 & 18.80 & IR25A & Iontropic receptor 25a \\ TRINITY\_DN13912\_cd\_g1\_i\_2 & 20.88 & 10.71 & 20.37 & IR25A & Iontropic receptor 25a \\
**SNMP** & & & & & \\ TRINITY\_DN1934\_cd\_g1\_i\_1 & 3567.61 & 3632.31 & 5117.35 & SNMP1 & Sensory neuron membrane protein 1 \\ TRINITY\_DN4669\_cd\_g1\_i\_1 & 176.96 & 204.92 & 206.30 & SNMP1 & Sensory neuron membrane protein 1 \\ TRINITY\_DN1867\_cd\_g2\_i\_1 & 86.72 & 83.98 & 50.41 & XP\_015114627.1 & Sensory neuron membrane protein 1 \\ cluster\_contig4264 & 70.20 & 73.23 & 46.97 & SNMP1 & Sensory neuron membrane protein 1 \\ cluster\_contig237 & 44.54 & 90.10 & 34.07 & SNMP1 & Sensory neuron membrane protein 1 \\ \hline \hline \end{tabular}  
* Normalized expression, TMM.  
\end{table} 
Table 3: Top five transcripts encoding olfactory proteins in Ashmead insects. Figure 3: Phylogenetic trees for OBPs (**A**) and CSPs (**B**). Ashmead proteins are highlighted in red, the percentage represents the bootstrap value, and the scale bar represents the evolutionary distance.