###### Abstract  
In light of the environmental problems stemming from chemical pesticides, a preparation system for an ozone-induced free radical solution was developed to replace chemical pesticides for disease control. The effective synthesis process parameters for the solution under experimental conditions were determined through a single-factor experiment. The mechanism by which the solution eradicates pathogenic bacteria was investigated using electron microscopy, and a disease prevention and control experiment was conducted. Under slightly acidic conditions, the redox potential of the solution was observed to be high, with an air intake of 0.5 L/min and a liquid intake of 1.45 L/min, while the concentration decayed slowly, with a liquid intake of 0.98 L/min. The solution's destructive effect on the bacteria's internal and external structures intensified with prolonged action time and an increased number of free radicals. A 1.5 mg/L solution and 5% imidalcoprid effectively reduced pest levels to grades 3 and 4, respectively. When the pH is 3, with air intake at 0.5 L/min and liquid intake at 0.98 L/min, the ozone-induced free radical solution exhibits strong oxidation and stability. At a concentration of 1.5 mg/L, the solution demonstrates a superior control effect on diseases and can partially replace chemical pesticides, offering a promising alternative for environmentally sustainable disease control. substitute pesticides; ozone; free radical; pest control; insecticide +
Footnote †: journal: Medical  
eventually transforming into a solution rich in liquid free radicals including \(\cdot OH\), \({}^{1}O_{2}\), \(\cdot H\)O\({}_{2}\)\({}^{-}\), and \(\cdot O_{2}\)\({}^{-}\). The solution exhibits several key characteristics: Firstly, it possesses a strong oxidizing property [9]. The \(\cdot OH\) radical in the solution possesses a significantly greater oxidizing capability than other chemical oxidants. The reaction's final products are CO\({}_{2}\), water, and other non-polluting substances. Secondly, it has a broad spectrum [10]. These free radicals initiate and participate in chain reactions, breaking down organic pollutants and attacking a wide array of microorganisms and pests. Thirdly, the free radical reaction rate constants range from 10\({}^{6}\) to 10\({}^{9}\) L\(\cdot\)mol\({}^{-1}\cdot\)s\({}^{-1}\), enabling rapid eradication of microorganisms and pests [11]. [PERSON] [12] introduced a green reaction device that synthesizes a free radical solution using UV and ozone, demonstrating superior biocidal effectiveness compared to traditional disinfectants. [PERSON] [13] utilized hot water and ozone water to effectively reduce the decay of papaya and achieve control over 50% of the disease. [PERSON] [14] discovered that spraying 1.5 ppm and 3.0 ppm ozone water not only effectively inhibited the occurrence of brown rot in nectarines and plums but also demonstrated no toxic effects on the fruits. [PERSON] [15] determined that ozone water effectively destroyed the salivary glands of Rhipicephalus sanguineus sensu lato and inhibited pest growth. Although the efficacy of ozone-induced free radical solutions as a substitute for pesticides in controlling agricultural pests and diseases has been widely confirmed, studies on the optimum process parameters for the synthesis of ozone-induced free radical solutions under experimental conditions, identifying the primary free radical types, and evaluating the actual disease control efficacy are limited. In view of the limitations of existing research, this study develops an efficient preparation system for an ozone-induced free radical solution, aiming to synthesize it as an alternative to traditional chemical pesticides. Initially, single-factor experiments determined the synthesis process parameters for the solution (pH, air intake, and liquid intake).