## 4 Conclusions  
The removal of dyes depends on physicochemical factors such as the concentration and size of the dye molecule, ionic charge, pH, and temperature. In this research, it was observed that the genotype and the preparation of the fungal biomass are relevant factors when looking for high removal percentages. In that sense, it was found that the removal of CR by the fresh biomass of both species of _Trichoderma_ studied was very similar. For MG, the highest percentage of removal was obtained with the live and dead biomass of _T. virens_. In the same way, the present study showed that after 24 h of contact time, fresh and dry biomass of _T. virens_ and _T. viriide_ had a higher CR removal capacity than activated carbon. Additionally, _T. virens_ showed a higher saturation capacity of the monolayer than  
Figure 6: Pseudo-second-order model fit plot for activated carbon and fungal dry biomass of _T. virens_ and _T. viriide_. (**A**) Congo red and (**B**) malachite green. \begin{table}
\begin{tabular}{c c c c c} \hline \hline
**Dye** & **Concentration (mg L\({}^{-1}\))** & **Adsorbent** & \(q_{e}\) **(mg g\({}^{-1}\))** & **K\({}_{2}\) (g mg\({}^{-1}\) h\({}^{-1}\))** & **R\({}^{2}\)** \\ \hline CR & 15 & Activated carbon & 0.525 & 6.819 & 0.999 \\ CR & 15 & _T. virens_ & 0.297 & 11.952 & 0.985 \\ CR & 15 & _T. vira_ & 0.209 & 10.381 & 0.991 \\ MG & 15 & Activated carbon & 0.700 & 6.215 & 1.000 \\ MG & 15 & _T. virens_ & 0.625 & 10.368 & 0.999 \\ MG & 15 & _T. vira_ & 0.619 & 3.505 & 0.999 \\ CR & 30 & Activated carbon & 0.900 & 3.691 & 0.999 \\ CR & 30 & _T. vira_ & 0.388 & 6.249 & 0.999 \\ CR & 30 & _T. [PERSON]_ & 0.600 & 7.030 & 0.991 \\ MG & 30 & Activated carbon & 1.548 & 2.773 & 1.000 \\ MG & 30 & _[PERSON]. [PERSON]_ & 1.425 & 4.870 & 0.999 \\ MG & 30 & _[PERSON]. [PERSON]_ & 1.458 & 10.890 & 0.999 \\ CR & 45 & Activated carbon & 1.592 & 3.022 & 0.999 \\ CR & 45 & _[PERSON]. [PERSON]_ & 0.813 & 5.943 & 0.999 \\ CR & 45 & _[PERSON]. [PERSON]_ & 0.908 & 9.418 & 0.988 \\ MG & 45 & Activated carbon & 2.168 & 2.058 & 1.000 \\ MG & 45 & _[PERSON]. [PERSON]_ & 2.006 & 2.982 & 0.999 \\ MG & 45 & _[PERSON]_ & 2.076 & 2.678 & 0.999 \\ CR & 60 & Activated carbon & 1.986 & 2.002 & 0.999 \\ CR & 60 & _[PERSON]_ & 1.160 & 3.389 & 0.986 \\ CR & 60 & _[PERSON]_ & 1.247 & 4.831 & 0.999 \\ MG & 60 & Activated carbon & 2.875 & 1.486 & 1.000 \\ MG & 60 & _T. virens_ & 2.583 & 2.904 & 0.999 \\ MG & 60 & _T. vira_ & 2.796 & 2.090 & 0.999 \\ CR & 75 & Activated carbon & 2.296 & 1.300 & 0.999 \\ CR & 75 & _T. virens_ & 1.060 & 3.709 & 0.998 \\ CR & 75 & _T. vira_ & 1.598 & 5.921 & 0.999 \\ MG & 75 & Activated carbon & 3.644 & 1.191 & 1.000 \\ MG & 75 & _T. virens_ & 3.206 & 2.828 & 0.999 \\ MG & 75 & _T. vira_ & 3.399 & 1.315 & 1.000 \\ CR & 90 & Activated carbon & 2.716 & 1.121 & 0.999 \\ CR & 90 & _T. virens_ & 2.207 & 1.561 & 0.988 \\ CR & 90 & _T. vira_ & 2.108 & 3.926 & 0.997 \\ MG & 90 & Activated carbon & 4.570 & 0.941 & 1.000 \\ MG & 90 & _T. virens_ & 3.951 & 1.408 & 1.000 \\ MG & 90 & _T. vira_ & 4.322 & 1.042 & 1.000 \\ \hline \hline \end{tabular}
\end{table} 
Table 5: Pseudo-second-order kinetic parameters for the removal of Congo red and malachite green by fungal dry biomass of _T. virens_ and _T. viriide_. _T. viride_ and activated carbon for MG removal. Thus, either fresh or dry biomass of _T. virens_ can represent a simple and cost-effective alternative for removing industrial dyes like CR and MG.  
Conceptualization, R.A.-D. and [PERSON]-M.; methodology, R.A.-D.; investigation and formal analysis, R.A.-D.; M.J.G.-M. and [PERSON]; data curation, [PERSON], [PERSON] and [PERSON] writing--original draft preparation, [PERSON] and [PERSON]; writing--review and editing, [PERSON]; [PERSON] and [PERSON]; funding acquisition, R.A.-D. All authors have read and agreed to the published version of the manuscript. This research was funded by Consejo Nacional de Ciencia y Tecnologia--CONACYT of Mexico, from the Basic Science Project-SEP, grant number 239601. Not applicable.