## 1 Introduction  
Gingival creeicular fluid (GCF) is physiologically produced in small amounts and its composition resembles blood serum. In a healthy state, GCF is responsible for leaching pathogens and toxic metabolites from the periodonontum. However, during inflammation, the microcapillary framework enhances the secretion of fluid containing various inflammatory biomarkers [1]. The inflammatory exudate components include bacteria, damaged tissue fragments, proteins, complement system components, immune cells, immunoglobulin, and inflammatory mediators [2; 3; 4]. Among the biochemical inflammatory mediators, proinflammatory and anti-inflammatory interleukins are listed. Interleukin-1 (IL-1) is one of the proinflammatory nuclear factor-\(\kappa\)B-dependent cytokines present in gingival fluid, and is responsible for acute phase response induction [5; 6]. The beta form (IL-1\(\beta\)), prevalent in periodontal tissues, is produced by macrophages [7]. [PERSON] et al. [8] showed that IL-1 is the most important biomarker associated with chronic periodontitis. Interleukin-1\(\beta\) is sometimes referred to as a mediator of periodontal bone loss, as it stimulates the formation of osteoclasts and affects bone resorption [9]. Furthermore, the rs1143634 polymorphism in the interleukin-1\(\beta\) gene seems to associated with an elevated risk of chronic periodontitis in Caucasian and Asian ethnicities, as well as mixed populations [10]. As a correlation between gingival fluid volume and the periodontitis stage exists [11], researchers have long searched for an effective, non-invasive method of collecting and measuring the amount of fluid to estimate the intensity and predict the occurrence of periodontal disease [2; 12; 13]. Construction of the Periotron device (Oraflow Inc., New York, NY, USA) was crucial and became considered the most accurate, non-invasive method for diagnosing the intensity of periodontitis, useful in subclinical symptom detection. The latest available model--Periotron 8010--additionally enables saliva testing [14]. The device uses the mechanism of an electric capacitor; it has an arm with two jaws that are its capacitor plates. Methylcellulose Periopaper strips (Oraflow Inc., New York, NY, USA) soaked in GCF are inserted between the jaws. The reading of the fluid volume is displayed in \"Periotron units\" (device units proportional to the change in capacitance of the capacitor). The result can be converted into SI units after plotting a standard curve using known fluid volumes [2]. Apart from periodontal diseases, the secretion of gingival fluid is influenced by various factors, such as medication intake, systemic diseases, hormonal status changes during the menstrual cycle [15], or treatment with orthodontic appliances [16]. Authors assumed that bruxism, repetitive activity of the masticatory muscles, may also impact this. Depending on the circadian occurrence of the habit, awake bruxism and sleep bruxism are diagnosed [17]. During sleep, rhythmic or non-rhythmic muscular activity is observed. Night grinding episodes are independent of the patient's consciousness. Awake bruxism often takes the form of permanent occlusal contact and manifests itself in voiceless clenching of teeth. The daily form depends mainly on emotional state and can be controlled by the patient [18; 19]. The etiopathogenesis of bruxism has not been fully elucidated, although the dominant factors leading to hypertonic are stress, anxiety, and genetic predisposition [20; 21; 22]. Other factors associated with bruxism include sleep disorders (e.g., obstructive sleep apnea), neurological (e.g., epilepsy) and endocrine disorders (e.g., hyper-myroidism), as well as gastroesophageal reflux disorder. Substances that promote bruxism include nicotine, caffeine, alcohol, and prescribed medications, such as antidepressants or antipsychotics[23]. The consequences of grinding and clenching are pathological wear of dental hard tissues, changes in the periodontium and oral mucosa, and stomatognathic system overload [24].