# Biological Activities of Selected Medicinal and Edible Plants Aqueous Infusions
## 3 Results and Discussion
### Antiproliferative Activity of Aqueous Herbal Infusions  
The antiproliferative activity of infusions was investigated against three human cancer cell lines: MD-MBA-231, T24 and A549 cells (Tables 4-6). Four different concentrations of stock solutions of each herbal infusion were tested (0.1 g/L, 0.25 g/L, 0.5 g/L, and 1 g/L) after 4, 24, 48 and 72 incubation periods. _G. macrornhizum_, _O spinosa_ and _A. millefolium_ showed the highest and dose-dependent antiproliferative effect against MD-MBA-231 cells after all incubation periods (Table 4). After 72 h of incubation, the antiproliferative effect was 54.99% for _G. macrornhizum_, 49.05% for _A. millefolium_ and 48.53% for _O. spinosa_. Compared to the cancer cells MD-MBA-231, the antiproliferative activity of the tested infusions against A549 cells was significantly higher. A very high (>88%) and dose-dependent antiproliferative activity was achieved by _G. macrornhizum_, _O. spinosa_, _A. millefolium_ and _P. aviculare_ (Table 5). In constrast to the low antiproliferative activity against MD-MBA-231 cells, the infusion of _P. aviculare_ showed a very high antiproliferative activity against A549 cells after 48 h and 72 h of incubation (78.50% and 94.40%). _G. macrornhizum_ showed a very high antiproliferative activity against A549 high antiproliferative activity against A549 cells already after 24 h of incubation (74.02%). The antiproliferative and dose-dependent activity of selected infusions against T24 cells is shown in Table 6. _G. macrornhizum_ (51.17% after 48 h and 96.20% after 72 h) and _O. spinosa_ (58.96% after 72 h) showed the highest activity. In general, the antiproliferative activity of the tested infusions against T24 cells was lower compared to other cancer cell lines tested, except for _G. macrornhizum_ (Tables 4-6). There are no data on the comparative antiproliferative activity of selected aqueous infusions against the tested cell lines and the available results are mostly based on the antiproliferative activity of different solvent extracts from selected plants. For example, many authors demonstrated the antiproliferative activity of the methanol extracts of _P. aviculare_, _V. thapsus_, _O. spinosa_, _A. millefolium_ against various cancer cell lines (breast cancer cells MCF-7, colon cancer cells CaCo2, liver cell lines HepG2 and HepG3B, glioblastoma cells A172, epidermoid carcinoma cells A431) [37; 41; 48]. [PERSON] et al. [50] reported that the hot water extract of _O. spinosa_ showed no inhibitory effect on T24 cells after 24 h of incubation. In our study, the infusion of _O spinosa_ also showed a low antiproliferative effect after 24 h of incubation (16.26%), but the effect was much higher after 48 h and 72 h of incubation (37.64% and 58.69%) (Table 6). _G. macrornhizum_ showed a very high and dose-dependent antiproliferative activity against all tested cancer cells (>50% for MD-MBA-231 cells and >90% for T24 and A549 cells after 72 h of incubation). The results obtained cannot be compared with similar studies as there are no data on the antiproliferative activity of _G. macrornhizum_ infusion against the tested cancer cell lines. Table 1 shows that _G. macrornhizum_ contains a very high content of quercetin and its derivatives (300.44 mg/L) and also a very high content of procyanidines (744.48 mg/L)