## 4 Conclusions  
In this study, we demonstrated that the MeOH extract of _C. ussuriensis_ roots exhibited potent inhibitory activity against BNA. We identified ussurienside I and tangshenoside I as the major constituents of the _C. ussuriensis_ root extract. The major compounds displayed significant inhibitory activity, with IC\({}_{50}\) values of 56.0 uM and 203.3 uM, respectively. All compounds showed mixed type-I inhibitory behavior. To the best of our knowledge, ussurienside I has never been linked to antibacterial action previously. Additionally, ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS) allowed us to identify phytochemical compounds present in the extracts. The \begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline
**No.** & **RT (min)** & **Identification** & \begin{tabular}{c} **Detected** \\ **Ion** \\ \end{tabular} & \begin{tabular}{c} **Calculated** \\ **Ion** \\ \end{tabular} & \begin{tabular}{c} **Adduct** \\ **Ion** \\ \end{tabular} & \begin{tabular}{c} **Error** \\ **(ppm)** \\ \end{tabular} & \
\begin{tabular}{c} **Fragment** \\ **Ion** \\ \end{tabular} &
\begin{tabular}{c} **Molecular** \\ **Formula** \\ \end{tabular} \\ \hline
1 & 1.74 & Citric acid & 191.0198 & 191.0192 & \([\mathrm{M}-\mathrm{H}]^{-}\) & 1.3 & 173.0, 111.0 & C\({}_{6}\)H\({}_{8}\)O\({}_{7}\) \\ \hline
2 & 5.14 & Tryptophan & 203.0827 & 203.0821 & \([\mathrm{M}-\mathrm{H}]^{-}\) & 0.5 &
\begin{tabular}{c} 159.0, 142.0, \\ 116.0, 74.0 \\ \end{tabular} & C\({}_{11}\)H\({}_{12}\)N\({}_{2}\)O\({}_{2}\) \\ \hline
3 & 8.51 & Tangshenoside I & 677.2297 & 677.2293 & \([\mathrm{M}-\mathrm{H}]^{-}\) & -0.2 &
\begin{tabular}{c} 497.1, 323.0, \\ 261.0, 99.0 \\ \end{tabular} & C\({}_{29}\)H\({}_{42}\)O\({}_{18}\) \\ \hline
4 & 8.85 & Unknown & 498.1549 & 498.1544 & \([\mathrm{M}-\mathrm{H}]^{-}\) & 0.02 &
\begin{tabular}{c} 328.0, 306.0, \\ 272.0, 143.0 \\ \end{tabular} & C\({}_{11}\)H\({}_{13}\)N\({}_{2}\)O\({}_{2}\) \\ \hline
5 & 9.84 & Ussurienside I & 515.1777 & 515.1765 & \([\mathrm{M}-\mathrm{H}]^{-}\) & 0.05 &
\begin{tabular}{c} 471.1, 371.1, \\ 161.0 \\ \end{tabular} & C\({}_{23}\)H\({}_{32}\)O\({}_{13}\) \\ \hline
6 & 10.88 & Ginkgolide B & 469.1355 & 469.1346 & \([\mathrm{M}+\mathrm{HCOO}]^{-}\) & 0.8 &
\begin{tabular}{c} 325.0, 225.0, \\ 163.0, \\ \end{tabular} & C\({}_{20}\)H\({}_{24}\)O\({}_{10}\) \\ \hline
7 & 11.58 & Unknown & 869.3100 & 869.3079 & \([\mathrm{M}-\mathrm{H}]^{-}\) & 1.8 &
\begin{tabular}{c} 563.2, 401.1, \\ 383.1, 161.0 \\ \end{tabular} & C\({}_{40}\)H\({}_{54}\)O\({}_{21}\) \\ \hline
8 & 12.16 & Lobetyolin & 441.1768 & 441.1761 & \([\mathrm{M}+\mathrm{HCOO}]^{-}\) & -0.32 &
\begin{tabular}{c} 373.1, 305.1, \\ 179.0, 112.9 \\ \end{tabular} & C\({}_{21}\)H\({}_{30}\)O\({}_{10}\) \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Compounds of _C. ussuriensis_ root extract identified using LC-Q-TOF-MS.  
Figure 4: LC-QTOF-MS chromatograms of compounds of _C. ussuriensis_ root extract. **(a)** PDA spectra; **(b)** MS spectra; **(c)** Tangshenoside I; **(d)** Ussurienside I.  
fact that there are two compounds in this plant shows that they could be used as starting points for producing antibacterial drugs. The following supporting information can be downloaded at: [[https://www.mdpi.com/article/10.3390/app12126254/s1](https://www.mdpi.com/article/10.3390/app12126254/s1)]([https://www.mdpi.com/article/10.3390/app12126254/s1](https://www.mdpi.com/article/10.3390/app12126254/s1)), Figures S1-S7: 1D and 2D NMR spectra of compounds **1** and **2**, Figure S8: Base peak chromatograms of _C. ussuriensis_ crude extract and identified secondary metabolites, Figure S9: IR spectrum of compound **1**, Table S1: \({}^{1}\)H-(700 MHz) and \({}^{13}\)C-NMR (125 MHz) spectra of compounds **1** and **2** in MeOD. S.C.K., H.S.W. and D.W.K. conceived the concept and designed the manuscript; S.C.K., J.H.K., J.Y. and Y.C.K. contributed critical information and helped revise the manuscript; S.C.K., H.S.W., J.W.K., Y.-S.K., J.Y.K. and D.W.K.