## 4 Conclusions  
In the present study, we have isolated twelve **(1-12)** components and identified 40 volatile compounds in Mexican propolis. The current study revealed the presence of antioxidant, antimicrobial, and cytotoxic phytochemicals [galangin (3); ferulic acid (10); syringic acid (11); and caffeic acid (12)] in EEP. It is concluded that EEP can be a potential addition in pharmaceutical products for the improvement of human health by contributing in the antioxidant defense system fighting against the production of free radicals. Mexico, being a megadiverse country, has numerous numbers of propolis differing in chemical composition. However, unfortunately it is still unexplored. \begin{table}
\begin{tabular}{c c c c c} \hline \hline  & **Name** & **Retention Index** & **Area \%** & **Method of Identification** \\ \hline
1 & \(\alpha\)-pinene & 939 & 8.046 & a, b, c \\
2 & \(\beta\)-pinene & 979 & 12.179 & a, b, c \\
3 & 1-octen-3-ol & 982 & 12.129 & a, b \\
4 & ethyl hexanoate & 1003 & 0.042 & a, b, c \\
5 & Octanal & 1006 & 1.273 & a, b \\
6 & Sylvestrene & 1030 & 3.022 & a, b \\
7 & acetophenone & 1076 & 0.486 & a, b \\
8 & 1-octanol & 1078 & 0.107 & a, b \\
9 & Thujone & 1102 & 0.276 & a, b \\
10 & p-cymene & 1082 & 0.291 & a, b \\
11 & Nonanal & 1100 & 18.829 & a, b, c \\
12 & 6-methyl-3,5-heptadiene-2-one & 1105 & 6.803 & a, b \\
13 & Eucalyptusptol & 1039 & 0.472 & a, b, c \\
14 & Camphor & 1146 & 0.583 & a, b, c \\
15 & _trans_-pinocamphone & 1162 & 2.386 & a, b \\
16 & _trans_-terpineol & 1163 & 0.102 & a, b \\
17 & \(p\)-menth-1,5-dien-8-ol & 1167 & 1.097 & a, b \\
18 & (\(2E\))-nonenal & 1168 & 1.097 & a, b \\
19 & \(m\)-cymen-8-ol & 1180 & 1.024 & a, b, c \\
20 & Unknown 1 & 1185 & 0.064 & - \\
21 & \(\alpha\)-terpineol & 1189 & 1.571 & a, b \\
22 & Myrtenol & 1193 & 1.571 & a, b \\
23 & p-fenchone & 1187 & 1.828 & a, b \\
24 & \(p\)-cymen-9-ol & 1207 & 3.108 & a, b, c \\
25 & neryl alcohol & 1217 & 10.135 & a, b \\
26 & trans-carveol & 1219 & 2.953 & a, b \\
27 & Citronellol & 1228 & 1.916 & a, b \\
28 & Ocimeneo & 1230 & 0.727 & a, b \\
29 & _cis_-chrysantenyl acetate & 1253 & 0.243 & a, b \\
30 & Geraniol & 1278 & 0.314 & a, b \\
31 & needehydroc arveol acetate & 1307 & 1.843 & a, b \\
32 & ethyl nonanoate & 1319 & 1.283 & a, b \\
33 & trans-carvyl acetate & 1337 & 0.357 & a, b \\
34 & \(\alpha\)-longipinene & 1352 & 0.181 & a, b \\
35 & \(\beta\)-cububene & 1388 & 0.248 & a, b \\
36 & \(\beta\)-bourbonene & 1398 & 0.217 & a, b \\
37 & n-decyl acetate & 1408 & 0.109 & a, b \\
38 & trans-granylacetone & 1455 & 0.429 & a, b \\
39 & \(\gamma\)-gelineene & 1485 & 0.103 & a, b \\
40 & \(\beta\)-bisabolene & 1509 & 0.290 & a, b \\ \hline \hline \end{tabular} a: Retention time; b: Retention index; c: Mass spectrum.  
\end{table} 
Table 5: Volatile components from Mexican propolisis GUA-4. GUA-4; Figure S3: Major volatile compounds of EEP GUA-4; Table S1: \({}^{1}\)H-RMN data of the flavonoids isolated from Mexican propulsion. [PERSON] performed the phytochemical study; [PERSON] conceived and designed the phytochemical experiments and wrote the paper; [PERSON] conceived and designed the in vitro experiments; [PERSON]-Previewed of the manuscript and contributed with in vitro experiments; [PERSON] contributed with the antimicrobial assays; [PERSON] reviewed the manuscript and contributed with in vitro experiments; [PERSON] reviewed of the manuscript and contributed with in vitro experiments. All authors have read and agreed to the published version of the manuscript. This research was funded by CONACyT CB-252006 and College of Chemistry, National Autonomous University of Mexico under the PAIP 5000-9138 Fellowship Scheme. We are in debt to Medico Veterinario Zootecnista. [PERSON] for technical assistance in propolisolis recollection. [PERSON] is a doctoral student from the Programa de Doctorado en Ciencias Quimicas, Universidad Nacional Autonoma de Mexico (UNAM), Facultad de Quimica, and recieved a CONACyT fellowship # 273461. The authors wish to thank the technical assistance of [PERSON] and [PERSON].