## 2 Materials and Methods
### Extraction with Organic Solvents
#### 2.5.2 Determination of Amount of Phenolic Compounds in the Pomace  
Full recovery of the phenolic compounds present in the pomace was achieved using a protocol described by [PERSON] [26]. The sample was homogenized and extracted with a 1% solution of hydrochloric acid in methanol. The solid remains were reextracted four times and the extracts obtained were connected.  
\begin{table}
\begin{tabular}{c c c c c c c c c c c c c} \hline \hline \multirow{3}{*}{**Sample**} & \multicolumn{4}{c}{**Carbon Dioxide Properties**} & \multicolumn{4}{c}{**Extract Composition**} & \multicolumn{4}{c}{**Activity Against**} \\ \cline{2-11}  & \(T\) (\(X_{1}\)) & \(p\) (\(X_{2}\)) & \(c\) (\(X_{3}\)) & \(\varrho\) (\(X_{4}\)) & **ACNs** & **TPC** & **DPPH\({}^{\bullet}\)** & **ABSTS\({}^{\bullet\bullet}\)** & **NO\({}^{\bullet}\)** & **O\({}_{2}\)\({}^{\bullet\bullet}\)** & **\({}^{\bullet\bullet}\)**OH** \\ \cline{2-11}  & \((^{\circ}\)C) & **(MPa)** & **(\% m/m)** & **(kg m\({}^{-3}\))** & **(kg m\({}^{-3}\))** & **(kg m\({}^{-3}\))** & **(kg m\({}^{-3}\))** & **(kg m\({}^{-3}\))** & **(kg m\({}^{-3}\))** & **(kg m\({}^{-3}\))** & **(kg m\({}^{-3}\))** \\ \hline
1 & 50 (0) & 10.0 (0) & 50 (0) & 398 (-0.28) & 410.3 & 711.4 & 1309.2 & 1227.8 & 24.7 & 648.8 & 41.15 \\
2 & 50 (0) & 12.5 (+1) & 80 (+1) & 596 (+0.33) & 749.1 & 1197.7 & 2017.2 & 2007.3 & 20.3 & 348.0 & 49.00 \\
3 & 50 (0) & 12.5 (+1) & 20 (-1) & 596 (+0.33) & 341.3 & 680.1 & 987.1 & 1214.1 & 16.2 & 621.8 & 28.41 \\
4 & 50 (0) & 7.5 (-1) & 80 (+1) & 17 (7-0.98) & 324.0 & 545.4 & - & - & - & - & - \\
5 & 50 (0) & 7.5 (-1) & 20 (-1) & 177 (-0.98) & 116.8 & 187.2 & 499.7 & 411.0 & 13.3 & 249.4 & 16.44 \\
6 & 50 (0) & 10.0 (0) & 50 (0) & 398 (-0.28) & 403.3 & 820.0 & - & - & - & - & - \\
7 & 65 (+1) & 10.0 (0) & 80 (+1) & 266 (-0.70) & 455.7 & 872. & - & - & - & - & - \\
8 & 65 (+1) & 10.0 (0) & 20 (-1) & 266 (-0.70) & 197.9 & 367.4 & - & - & - & - & - \\
9 & 35 (-1) & 10.0 (0) & 80 (+1) & 766 (+0.86) & 102.4 & 1520.7 & 2907.0 & 3384.1 & 29.0 & 1138.4 & 72.24 \\
10 & 35 (-1) & 10.0 (0) & 20 (-1) & 766 (+0.86) & 574.8 & 102.0 & - & - & - & - & - \\
11 & 50 (0) & 10.0 (0) & 50 (0) & 398 (-0.28) & 422.4 & 681.4 & - & - & - & - & - \\
12 & 65 (+1) & 12.5 (+1) & 50 (0) & 419 (+0.22) & 437.5 & 801.4 & - & - & - & - & - \\
13 & 65 (+1) & 7.5 (-1) & 50 (0) & 169 (-1.00) & 201.6 & 356.4 & 671.0 & 702.4 & 187 & 328.4 & 27.28 \\
14 & 35 (-1) & 12.5 (+1) & 50 (0) & 812 (+1.00) & 907.7 & 1429.9 & - & - & - & - & - \\
15 & 35 (-1) & 7.5 (-1) & 50 (0) & 207 (-0.88) & 406.5 & 641.1 & - & - & - & - & - \\
5 & & full extraction of polyphenols with methanol & 1812.4 & 3204.4 & 6418.3 & 6877.4 & 54.7 & 2900.7 & 135.77 \\
7 & ethanol extract (ethanol-solid ratio 50% m/m) & 245.3 & 433.0 & 923.1 & 908.6 & 11.9 & 341.1 & 30.12 \\ \hline \hline \end{tabular}
\end{table}
Table 1: Composition and activity against selected radicals of the chokeberry extracts obtained under different extraction parameters. Masses refer to the pomace. Abbr: \(T=\) temperature, \(p=\) pressure, \(c\) ethyl alcohol addition, \(\varrho=\) density, \(X_{i}=\) normalized process parameters (in the range from \(-1\) to \(+1\)), \(ACNs\) = anthocyanins, TPC = total phenolic content, CSG = cyanidin-3-O-glucose, GA = gallic acid, curc = curcumin, querc = quercetin, sorb = sorbitol.