## 2 Materials and Methods
### Antioxidant Activity of Dipteryx Punctata Extracts by TLC and DPPH Free Radical Scavenging  
The ethanolic extracts obtained were also evaluated by TLC for their antioxidant potential, according to an adapted methodology [17], using the same elution system for the different classes of compounds, and ABTS (2,2\({}^{\prime}\)-azinobis (3-ethylbenzothazoline-6-sulfonic acid)) and DPPH (2,2-diphenyl-l-picrylhydrazyl) radicals as developers. Plates developed with ABTS (7 mM) had ascorbic acid as standard (1000 ug.mL\({}^{-1}\)) and for those developed with methanolic solution DPPH (0.2%), the standard used was gallic acid (100 ug.mL\({}^{-1}\)).  
The antioxidant potential resulting from the reduction in ABTS was evaluated by the presence of white spots on a greenish background after 48 h and that of DPPH by the presence of light-yellow spots against purple staining, after 2 h. All plates were kept in the dark during this period.  
After qualitative verification of the antioxidant action on the plaques, the selected quantitative method was DPPH free radical capture with some modifications [30], as only this analysis enables the necessary extract concentrations to be obtained for a 50% reduction in radicals [31]. Representative samples from all collection areas were weighed and grouped; then, the sample from each part of the plate was diluted and aliquots added to test tubes to obtain concentrations of 40, 70, 200, 350, and 500 ug.mL\({}^{-1}\) for leaf and fruit residue extracts and 230, 800, 1500, 2500, and 3000 ug.mL\({}^{-1}\) for branch and seed extracts, up to a volume of 600 uL in methyl alcohol P. A.  
After this process, 2.4 mL of the DPPH radical solution (29 ug.mL\({}^{-1}\)) diluted in methyl alcohol P.A. were added to the tubes and homogenized in a shaker. For negative control, 600 uL of methyl alcohol and 2.4 mL of DPPH radical were used. All analyses were performed in triplicate. The two readings were performed with a UV/visible spectrophotometer (\(\lambda\) = 516 nm) and monitored at intervals of 40 min (80 min in total) until the absorbance stabilized. The antioxidant action was verified by observing the color change in the sample (purple or violet) to light yellow or pale violet after that period in the dark.  
The DPPH index was calculated using the equation: I (%) = [(Abs0 \(-\) Abs1)/Abs0] \(\times\) 100, where Abs0 is the absorbance of the blank and Abs1 is the absorbance of the sample. Regression analysis was performed using the SISVAR statistical software, version 5.6, Build 90 [26]. Based on the relationship between the concentrations tested and the percentage of inhibition calculated, adjusted logarithmic functions were obtained with representation of each part of the plant given via y = a ln (x) \(\pm\) b (a and b = constants; ln = natural logarithmic function) for the calculation of IC50 values (extract concentration necessary to reduce 50% of the DPPH radical), where IC50 = EXP((50 \(-\) b)/a). The logarithmic model was used to plot the graphs in the electronic spreadsheet program, Microsoft Office Excel 365 (Microsoft Corporation, an American Company located in Redmond, Washington), presenting five points plotted for each extract (leaves, branches, residues, and seeds).