# The Immobilization of Lipases on Porous Support by Adsorption and Hydrophobic Interaction Method
## 3 Porous Carriers Used for the Immobilization of Lipase
### Criteria
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Lipase Source** & **Support** & **Surface Area** & **Particle Size** & **Pore Size** & **Reference** \\ \hline \multirow{6}{*}{\begin{tabular}{c} Phospholipase D from _Stripomyces_ sp. \\ \end{tabular} } & & & Ultramacroproe & & \\  & & & & (5-50 \(\upmu\)m) & & \\  & & & Macropore & & \\  & & hierarchical porous & 225–156 m\({}^{2}\)/g & (0.1-5 \(\upmu\)m); & - & [37] \\  & polymers (HPSs) & & Mesoporce & & \\  & & & (10-50 \(\upmu\)m) & & \\ \hline \multirow{6}{*}{\begin{tabular}{c} Lipase B from _Candidata enterica_ \\ (CALB) \\ \end{tabular} } & & & & \\  & Methyl methacrylate resins & 500 m\({}^{2}\)/g & 560–710 \(\upmu\)m & 250 Å & [61] \\  & & & & \\ \hline CALB & Polystyrene resins & - & 350–600 \(\upmu\)m & 300–1000 Å & [62] \\ \hline AKL 13 lipase from _Bacillus subtilis_ (BsL) & Mesoporous cuttlebone powder of Sepia officinalis (CBP) & 1.631 m\({}^{2}\)/g & 89.95 \(\upmu\)m & - & [63] \\ \hline \multirow{6}{*}{\begin{tabular}{c} _Candidata rugost_ \\ (OF360) \\ \end{tabular} } & & EP100: 50–90 m\({}^{2}\)/g & \(<\)200 \(\upmu\)m & 0.1–1.0 \(\upmu\)m & \\  & & EP400: 20–40 m\({}^{2}\)/g & \(<\)400 \(\upmu\)m & 0.1–0.5 \(\upmu\)m & [64] \\ \hline \multirow{6}{*}{\begin{tabular}{c} _Thramomyces_ \\ _lamingness_ \\ lipase (TLL) \\ \end{tabular} } & & & & \\  & & & & \\ \hline \multirow{6}{*}{\begin{tabular}{c} _Muocr mithei_ \\ _Rizopus orgae_ \\ \end{tabular} } & & & & & \\  & & & 502 m\({}^{2}\)/g & 1000 nm & 9.3 nm & \\ \cline{1-1} \cline{3-5}  & Mesoporous silica (MPS) & MPS-300: 606 m\({}^{2}\)/g & 300 nm & 9.4 nm & [66] \\ \cline{1-1} \cline{3-5}  & MPS-40: 463 m\({}^{2}\)/g & 40 nm & 9.1 nm & \\ \hline \multirow{6}{*}{\begin{tabular}{c} _Candidata rugosa_ \\ (C18-MSNs) \\ \end{tabular} } & & & & & \\ \cline{1-1} \cline{3-5}  & Mesoporous silica (PMC) & 500 m\({}^{2}\)/g & 300–700 \(\upmu\)m & 170 Å & [68] \\ \cline{1-1}  & Mesoporous silica (PMC) & & & \\ \cline{1-1}  & Mesoporous silica (PMC) & & & \\ \cline{1-1}  & Mesoporous silica (PMC) & & & \\ \cline{1-1}  & Mesoporous silica (PMC) & & & \\ \hline \multirow{6}{*}{\begin{tabular}{c} _Burbhelderia c capacia_ \\ _Mavor jacunicis_ \\ lipase \\ \end{tabular} } & & & & 570 m\({}^{2}\)/g & 300 \(\upmu\)m & 200 Å & [69] \\ \cline{1-1}  & Poly(methylmethacrylate-co & & & \\ \cline{1-1}  & divinylbenzene)-encapsulated & 12 m\({}^{2}\)/g & - & 170 nm & [70] \\ \cline{1-1}  & porous magnetic microspheres & & & \\ \hline \multirow{6}{*}{\begin{tabular}{c} Candida sp. L3170 \\ resin \\ \end{tabular} } & & & & & \\ \cline{1-1} \cline{3-5}  & Purolite \& A109, polystyrene & & & \\ \cline{1-1}  & macroporous resin & - & 400 \(\upmu\)m & - & [31] \\ \hline \multirow{6}{*}{\begin{tabular}{c} CALB \\ \end{tabular} } & & & & & \\ \cline{1-1}  & Styrene-divinylbenzene MCI & & & \\ \cline{1-1}  & GEL CHP20P porous support & \(>\)500 m\({}^{2}\)/g & 75–150 \(\upmu\)m & 400–600 Å & [60] \\ \cline{1-1} \cline{3-5}  & Lipases A and B from _Candidata enterica_ & Mesoporous carbon beads & \(>\)800 m\({}^{2}\)/g & 0.5–1 mm & - & [71] \\ \hline \multirow{6}{*}{\begin{tabular}{c} CALB \\ \end{tabular} } & & & & & \\ \cline{1-1}  & Pore-expanded mesoporous silica (SBA-15) & 526 m\({}^{2}\)/g & - & 14 nm & [72] \\ \hline \multirow{6}{*}{\begin{tabular}{c} CALB \\ \end{tabular} } & & & & & \\ \cline{1-1}  & Accurel MP (a macroporous & 78.92 cm\({}^{2}\)/g & \(\upmu\)m & 10 nm & \\ \cline{1-1}  & propylene) & & & \\ \cline{1-1}  & MP1001: \(<\)1000 & 8 \(\upmu\)m & [59] \\ \hline \multirow{6}{*}{\begin{tabular}{c} _Thramomyces_ \\ _lamingosus_ \\ \end{tabular} } & & & & & \\ \cline{1-1}  & Mesoporous Napolea Sc-18 & 116 m\({}^{2}\)/g & - & 616 Å & [58] \\ \cline{1-1}  & Mesoporous silica (SBA-15) & 60–80 m\({}^{2}\)/g & & 100–200 Å & [58] \\ \hline \multirow{6}{*}{\begin{tabular}{c} Heterologous \\ _Rizopus oryzae_ \\ \end{tabular} } & & & & & \\ \cline{1-1}  & Accurel MP 1000 & 70 m\({}^{2}\)/g & 50–1000 \(\upmu\)m & 2000 Å & [8] \\ \cline{1-1}  & Occ 1600 & & & & \\ \cline{1-1}  & Mesoporous silica (HMS) & 315–100 \(\upmu\)m & 15 Å & \\ \hline \multirow{6}{*}{\
\begin{tabular}{c} _Burbhelderia ambydia_ \\ (YCJ01) \\ \end{tabular} } & & & & & \\ \cline{1-1}  & Presoporous silica (PMC) & 38.02 m\({}^{2}\)/g & - & 16.25 nm & [73] \\ \cline{1-1}  & poly(ethylene oxide) & & & \\ \hline \multirow{6}{*}{
\begin{tabular}{c} _Burbhelderia c capacia_ \\ _Camdata rugosa_ \\ \end{tabular} } & & & & & \\ \cline{1-1}  & Mesoporous silica (HMS) & 399 m\({}^{2}\)/g & - & 12 nm & [75] \\ \hline \hline \end{tabular}
\end{table}