## 1 Introduction  
Peripheral nerve plasticity and its response to physical stress play a crucial role in clinical care for individuals with nerve dysfunctions. Mechanosensitivity of neural tissues is a key protective mechanism, allowing nerves to adapt to stress, tension variations, or compression, which can lead to symptoms such as pain, paresthesia, or restricted movement [1, 2]. Increased nerve sensitivity requires less force to provoke discomfort, resulting in an amplified response. Understanding and managing mechanosensitivity is essential in both therapeutic and rehabilitation contexts [2, 3, 4]. The mechanosensitivity of nerves can be assessed clinically by palpating neural structures and/or applying tension to the nervous system through neurodynamic tests, which demonstrate good reliability, precision, and diagnostic accuracy [2; 5; 6]. These tests are particularly useful for identifying nerve-related dysfunctions and guiding treatment strategies. The upper limb neurodynamic test 1 (ULNT1) is a provocative test designed to evaluate the mechanosensitivity of the brachial plexus and median nerve. This test involves a series of sequential movements that promote sliding and apply progressive tension to the brachial plexus and median nerve [1; 4; 7]. Key factors to consider during the assessment include the location of symptoms, resistance to movement, range of motion, and sensory and structural differentiation maneuvers [8]. Assessing the mechanosensitivity of the median nerve is particularly relevant in the context of cupping therapy because this nerve is often implicated in common musculoskeletal conditions such as carpal tunnel syndrome and cervical radiiculopathy. These conditions are characterized by increased nerve mechanosensitivity, which can lead to pain, tingling, and functional limitations [2; 3]. Understanding how cupping therapy affects nerve mechanosensitivity could provide valuable insights into its potential therapeutic applications for these and other nerve-related disorders. Cupping therapy is an ancient technique with documented applications spanning thousands of years [9]. This non-invasive mechanical massage technique utilizes negative pressure to create a localized vacuum, promoting blood circulation and mobilizing underlying tissues [10; 11]. Research suggests that cupping therapy can reduce fascial and muscular tension [12], increase pain thresholds [12; 13], enhance local circulation [14], and modulate inflammation [15]. These effects suggest potential benefits for neuromuscular conditions, particularly those involving mechanosensitivity alterations. Despite its widespread use, the impact of cupping therapy on peripheral nerve mechanosensitivity remains unclear. While previous studies have demonstrated its efficacy in increasing range of motion [16] and alleviating musculoskeletal pain [12; 13], no research has specifically investigated its immediate effects on the median nerve's mechanosensitivity. Given that musculoskeletal conditions often involve nerve-related symptoms, exploring this relationship is crucial. This represents a significant research gap, as musculoskeletal conditions often involve nerve-related symptoms, and understanding the relationship between cupping therapy and nerve mechanosensitivity could enhance its clinical application. Recent studies have further emphasized the importance of neurodynamics in physiotherapy, particularly in the management of nerve-related pain and dysfunction. For example, [PERSON] et al. (2012) demonstrated that neural tissue management can provide immediate clinically relevant benefits for patients with nerve-related neck and arm pain [8]. Additionally, [PERSON] et al. (2009) highlighted the reliability of neurodynamic tests in assessing upper limb nerve function [5]. These findings underscore the need for further research into interventions, such as cupping therapy, that may influence nerve mechanosensitivity.