## 3 Results  
All used OAs displayed a high antibiofilm activity, e.g., low values of MBEC, in the standard setting of 96-well culturing plates. Results are presented in Table 1. \begin{table}
\begin{tabular}{c c c c c c c} \hline \multirow{2}{*}{**Antistieptics**} & \multicolumn{5}{c}{**MBEC (\% of Dilution of Specific OA Product)**} \\ \cline{2-7}  & S. aureus & E. faecalis & P. aeruginosa & C. albicans & Str. mutans & A.cinomycetemcomitans \\ \hline B & 3.125 & 3.125 & 6.25 & 1.56 & 3.125 & 3.125 \\ PO & 0.098 & 0.098 & 3.125 & 3.125 & 0.098 & 0.78 \\ P & 0.098 & 0.195 & 12.5 & 3.125 & 0.195 & 0.390 \\ \hline \end{tabular}  
* B—Braunol, PO—ProttOral, P—Prottosan. MBEC is presented in the form of the percentage of dilution of the specific oral antiseptic product.  
\end{table} 
Table 1: Minimal biofilm eradication concentrations (MBECs) of oral antiseptic against tested strains. It should be noted that oral antiseptics containing polihexanide (PO and P) were more active against all microbial strains analyzed (with the exception of _C. albicans_) than oral antiseptic containing povidone-iodine (B) in this setting. Also, the PO and P MBEC values obtained regarding a specific strain were the same or differed with maximally one level of dilution. Using antiseptics proven in standard test settings (e.g., microdilution assays in 96-well plates), we chemisorbed these compounds to BC and tested the combination product in another standard microbiological assay (disc-diffusion methodology) to assess the antimicrobial activity. The only difference was that instead of the antibiotic-saturated discs, BC membranes with chemisorbed antiseptics were used. The average weight of wet BC dressings was \(276\pm 60\) mg, while their dry weight was \(2.4\pm 0.4\) mg. The average weight of water trapped within native BC dressings was estimated for 273 \(\mu\)L. The average volume of the filtrate obtained after centrifugation of OA-BC was \(196.5\pm 22.7\), \(213.16\pm 45.45\), and \(240\pm 40\)\(\mu\)L for B, P, and PO, respectively. The estimated concentration of particular OAs in filtrates compared with working solutions was \(70\pm 11\%\), \(68\pm 9\%\), and \(64\pm 11\%\) for B, P, and PO, respectively. Knowing the concentration of the active substance in working solutions of particular OAs, the amount of drug incorporated in B-BC could thus be estimated for \(\approx\)10.5 mg of povidone-iodine complex and \(\approx\)1 mg of active iodine (according to the manufacturer, PVP-I complex contains 10% of active iodine element; please refer to Section 2.2). For P and PO-BC discs, analogical values were estimated for 0.14 mg and 0.15 mg, respectively. The appearance of BC membranes used for this experimental setting, together with their micro-structure, is presented in Figure 2, while specific AOs released from BC dressings is presented in Figures 3 and 4. Figure 3: Release of PVP-iodine from the BC dressing. The assay was performed for 300 min. The first 100 min are presented for figure clarity. Figure 2: Presentation of the BC carriers (**A**) and their porous, mesh-like microscopic structure (**B**). An EVO MA microscope was used at 1360\(\times\) magnification. Antimicrobial activity and the repeatability of results is shown in Figure 5 with _S. aureus_ as an exemplar. The summarized results of the antimicrobial activity of BC antiseptics against all strains analyzed are presented in Table 2.  
Figure 4: Release of polihexanidine from the BC dressing. The assay was performed for 300 min. The first 100 min are presented for figure clarity. Figure 5: Antimicrobial activity of BC antiseptic carriers against _S. aureus_. P—Prontosan, PO—Prontoral, B—Braunol, NaCl—control setting, i.e., BC saturated with saline. 1.2–1.3: iterations of the experiment purposely presented in this figure to visualize the high repeatability of the obtained growth inhibition zones.