## 4 Materials and Methods  
### General Procedures and Reagents  
All reagents were purchased as reagent grade and used without further purification. Solvents for peptide synthesis and RP-HPLC were purchased as synthesis-grade and HPLC-grade, respectively. Polystyrene AM NH\({}_{2}\) (AM PS), TentaGel(r)-S-NH\({}_{2}\) resin with alternative particle size of 90 \(\upmu\)m (used in automated peptide synthesiser) and 130 \(\upmu\)m (used in flow chemistry vessel) were purchased from RAPP polymere (Tubingen, Germany). ChemMatrix(r) resin and Fmoc-Gly-3-(4-hydroxymethylphenoxy)propionic acid (Fmoc-Gly HMPpler) were purchased from Polypeptide Laboratories Group (Limhamn, Sweden). 4-(Hydrooxymethyl)phenoxyacetic acid (HMPA), 4-(4-hydroxymethyl-3-methoxyphenoxy)butyric acid (HMPB), 4-(Dimethylamine)pyridine (DMAP), Fmoc-Lys(Mtt)-OH, 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), acetic acid (Ac\({}_{2}\)O) and polymycin B sulfate were purchased from AK Scientific (Union City, California). Amphotericin B (from _Streptomyces_), human serum (from male AB serum), \(N\),\(N\)-disopropyl ethylamine (DIPEA), Fmoc-Dab(Dde)-OH, 2,4,6-trimethylpyridine (\(sym\)-collidine), \(N\),\(N^{\prime}\)-disopropylcarbodimide (DIC), tristiopropylane (TIPS), piperidine, 3,6-diox-1,8-octanedithiol (DODT), trichloroacetic acid (TCA) and formic acid (FA) were purchased from Sigma-Aldrich (St Louis, MO, USA). Diethyl ether (Et\({}_{2}\)O) was purchased from Avantor Performance Materials (Center Valley, USA). Trifluoroacetic acid (TFA) was purchased from Oakwood Chemicals (Estill, SC, USA). \(N\)-Methylmorpholine (NMM), 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazole [4,5-b]pyridinium 3-oxide hexafluorophosphate (HATU) and all Fmoc-amino acids were purchased from CS Bio China (Shanghai, China). Fmoc-Asp(O-2-PhiPr)-OH and Fmoc-Glu(O-2-PhiPr)-OH, 6-chloro-1-hydroxybenzotriazole (6-Cl-HOBt), Fmoc-Ser(Hu)-Thr(Psi(Me,Me)pro)-OH was purchased from Apptice (Louisville, Kentucky, USA). \(N\),\(N\)-dimethylformamide (DMF; AR grade) and acetonitrile (CH\({}_{3}\)CN, HPLC grade) were purchased from Thermo Scientific (Hampshire, NH, USA). Dichloromethane (CH\({}_{2}\)Cl\({}_{2}\)) and iodine (I\({}_{2}\)) were purchased from ECP Limited (Auckland, New Zealand). Polypropylene 96-well flat bottom plates were purchased from Greiner Bio-One (Kremsmunster, Austria). Milli-Q high purity deionised water (MQ H\({}_{2}\)O) was available from a Sartorius Arium(r) Pro Ultrapure Water System from Sartorius Stedim Biotech (Gottingen, Germany). Microwave reactions were carried out in a Biotage(r) (Uppsala, Sweden) Initiator Alstra(tm) microwave peptide synthesiser at variable temperatures. Room-temperature automated peptide synthesis was performed on a Tribute(r) automated synthesiser at Gyros Protein Technologies (Tucson, Arizona) at room temperature. Manual flow chemistry was carried out in a custom-made metal reactor packed with the desired resin. Reagents were delivered via a 5-mL stainless steel heating loop submerged in a water bath (65 \({}^{\circ}\)C) to the reaction vessel, also submerged in a water bath (65 \({}^{\circ}\)C). All reagents were delivered at a constant flow rate of 15 mL/min. Reverse phase high performance liquid chromatography (RP-HPLC) was performed on a Thermo Scientific (Waltham, MA, USA) Dionec Ultimate 3000 HPLC equipped with a four channel UV Detector at 210, 225, 254 and 280 nm using either an analytical column Waters (Milford, MA, USA) XTerra(r) MS C18, (5 \(\upmu\)M; 4.6 \(\times\) 150 mm) at a flow rate of 1 mL min\({}^{-1}\) or a semi-preparative column Phenomenex(r) (Torrance, CA, USA), Gemini C18, 5 mm; 10 \(\times\) 250 mm) at a flow rate of 4.5 mL min\({}^{-1}\) operated at room temperature. For both analytical and semi-preparative RP-HPLC, the solvents used were as follows: solvent A = 0.1% TFA in water (MQ H\({}_{2}\)O) and solvent B = 0.1% TFA in MeCN. For analytical RP-HPLC, the gradient employed was 5-65% of solvent B over 60 min at a flow rate of 1 mL/min, unless specified otherwise.