# Steering the Clinical Translation of Delivery Systems for Drugs and Health Products
## 3 Poster Presentations
### Polysaccharide Hydrogels for the Stabilization and Controlled Release of Ionic Cargo
###### Abstract
To protect OV against NAbs and enhance antitumor efficacy we decided to encapsulate OVs into extracellular vesicles (EV-OV). EVs are naturally occurring cargo delivery vesicles with the ability to selectively target the tumor tissue originating the vesicles and to deliver a wide variety of macromolecules. We demonstrated that mesothelioma derived-complex EV-OV resulted in more potent anti-neoplastic activity compared to the virus or EVs alone. Encapsulation seems to protect the OV from immune disruption through their encapsulation into EVs. Furthermore, a program of immunogenic cell death was triggered by the treatments (EV-OV, OV) suggesting that mesothelioma derived-EV-Virus formulations, but not EVs alone, were able to counteract the growth of tumor cells and induce immunogenic cell death. The presented approach might be a way for a systemic delivery of oncolytic viruses and other drug combinations through EVs for the treatment of cancer malignancies, including mesothelioma. **Acknowledgments:** MINIATURA (2018/02/X/NZ7/00727) funded by National Science Center, Poland (L.K. ); EU COST action CA17140 (M.G. & L.K. ); 1 BWBW/19 funded by National Institute of Public Health--National Institute of Hygiene (NIPH-NIH), Poland (L.K.). Development of Poly (Lactide-co-glycolide) Nanoparticles Decorated with Hyluronic Acid and Loaded with Gold for Visualization and Treatment of Osteoarthritis  
[PERSON] 1,*, [PERSON] 2, [PERSON] 3, [PERSON] 4, [PERSON] 5, [PERSON] 6, [PERSON] 2, [PERSON] 1 and [PERSON] 1  
1 School of Pharmacy, Drug Delivery Division, University of Camerino, 62032 Camerino (MC), Italy 2 Translational Nanobiomaterials and Imaging (TNI) group, Radiology department, Leiden University Medical Centrum, 2333 ZA Leiden, The Netherlands 3 Biotechnology and Food Engineering dept, Technion Israel Institute of Technology, 3200003 Haifa, Israel 4 Department of Orthopedics, Utrecht Medical Center, 3584 CX Utrecht, The Netherlands 5 Department of Radiology & Nuclear Medicine, Erasmus University Medical Center, 3000 CA Rotterdam, The Netherlands 6 Percuros B.V., 2333 CL Leiden, The Netherlands * Correspondence: [EMAIL_ADDRESS]  
Osteoarthritis (OA) represents the most common chronic and degenerative disease. Until now, no specific cures for OA are available, but intra-articular (IA) drug delivery could be a very promising treatment [37]. This treatment allows to minimize the amount of drug for local administration and have high potency and minimal side effects. In particular, glucocorticoids and sodium hyaluronate/hyaluronic acid (HA) are the broadly class of compounds used for OA treatment via IA injection. However, the efficacy of these treatments is relatively short, due to the rapid clearance and short residence time of the glucocorticoids (1-2 h) and HA (22-26 h) in the site of action [38]. The present investigation aimed to develop innovative nanoparticles (NPs) drug delivery system-based poly (D, L-lactide-co-glycolide) (PLGA) functionalized with sodium hyaluronate, which is used as a ligand to target specific cell receptors (CD44) for OA treatments [39; 40]. The PLGA (RG 503H, 39000 Da) was activated by DCC coupling and reacted with the complex of sodium hyaluronate(21 kDa-40 kDa)/dm PEG (dm PEG; 2000 Da) in anhydrous DMSO for two days at room temperature. PLGA-HA copolymer was synthesized with a yield of 67%. \({}^{1}\)H-NMR in DMSO and D\({}_{2}\)O and FT-IR spectrum pointed out the formation of PLGA-HA copolymer. The amount of covalent grafted HA was 75% obtained by CTAB assay. By using PLGA-HA copolymer, NPs were prepared by a double emulsion solvent evaporation method and co-encapsulated with near-infrared dye (NIR) and gold NPs of 20 nm diameter. NIR dye was useful for visualizing the NPs _in vitro_ and _in vivo_ through molecular imaging, while gold was used as a contrast agent for _in vivo_\(\upmu\)CT scan [41; 42]. NPs of PLGA-HA showed a particle size around 200 nm, a low polydispersity index and the zeta potential of \(-23.15\pm 1.67\) mV.