## 3 Results
### Protective Effect of Different Varieties of RLEE on UVB Induced L929 Fibroblast Injury  
In order to further investigate the protective effect of RLEE from different varieties of on L929 fibroblasts under UVB irradiation, fibroblasts were treated according to the method section. Firstly, the CCK-8 kit was used to measure the fibroblast viability after UVB exposure with or without 50 \(\upmu\)g/mL RLEE pre-protection (Figure 6A). Compared with the control group, UVB exposure could induce dramatic cell viability decreasing caused by oxidative stress and leading to cell injury and apoptosis. When pre-protected by Autumn Britten and Autumn Bliss RLEE, UVB exposure induced fibroblast toxicity was significantly reduced (Figure 6A, \(p<0.001\)). Additionally, as shown in Figure 6B, the levels of SOD in L929 fibroblast cells showed significant decrease in UVB groups compared with control counterparts. Moreover, SOD activity was significantly increased in the RLEE pre-protected group compared to the UVB group, Autumn Britten increased SOD activity to 120.32 \(\pm\) 0.63 U/mgprot compared to the UVB exposed group (Figure 6B). As illustrated in Figure 6C, the levels of GSH in L929 fibroblast cells showed significant reduction in the UVB group, compared with the control group, RLEE pre-protection increased the GSH level of fibroblasts, among which Autumn Britten and Autumn Bliss had the most significant effect (\(p<0.01\)). As shown in Figure 6D, the levels of CAT in L929 fibroblast cells exhibited significant inhibition in UVB and UVB + RLEE groups, while cells from the Autumn Britten RLEE and the Autumn Bliss RLEE pre-protection groups showed significant elevation (\(p<0.01\)) compared to their control counterparts.  
Figure 6: Cell viability and antioxidant enzyme activity of L929 fibroblasts treated with different varieties of RLEE (50 \(\upmu\)g/mL); (**A**): cell viability (**B**): SOD Activity (**C**): GSH Activity (**D**): CAT Activity. SOD activities were expressed in terms of U/mg protein, U means that when the SOD inhibition rate reaches 50% in the reaction system, the corresponding enzyme amount is an activity unit (U); GSH activity were expressed in terms of \(\upmu\)mol/g protein; CAT activity were expressed in terms of U/mg protein, U means 1 \(\upmu\)mol of H\({}_{2}\)O\({}_{2}\) per milligram of cellular protein per second is an activity unit (U). (CK: control group: untreated cells; UVB: exposed only to UVB without RLEE protection; **\(p<0.05\), ***\(p<0.01\), significant difference compared to CK, \({}^{*}\)\(p<0.05\), ***\(p<0.01\), significant difference compared to UVB group).  
L929 fibroblasts were pre-protected with RLEE of different varieties (50 \(\upmu\)g/mL) for 12 h. After UVB exposure, they continued to be cultured for 12 h and then treated with the ROS kit to observe the fluorescence using inverted fluorescence microscopy (Figure 7). Compared with the blank group, the fluorescence intensity of fibroblasts after UVB exposure increased significantly, this indicated the content of ROS in cells increased significantly. The fluorescence intensity of cells treated with different varieties of RLEE decreased gradually, and the fluorescence intensity of cells protected by 50 \(\upmu\)g/mL Autumn Britten RLEE decreased most after exposure to UVB.