## 2 Results and Discussion
### Analysis of the Volatile Fraction
#### 2.1.3 GC-MS Analysis of EOs
In addition to myrcene, other abundant constituents were (\(E\))-aryophyllene (16.0%) and \(\alpha\)-pinene (11.0%) in Fresh Mountain, and terpinolene (11.8%), (\(E\))-aryophyllene (11.4%), \(\alpha\)-pinene (11.3%) and limonene (10.2%) in White Shark. Myrcene was followed by \(\alpha\)-pinene (12.5%) and (\(E\))-aryophyllene (10.7%) in Lemon Conti Kush. Differently from the latter, the Lemon Conti Kush New EO presented terpinolene as the predominant component, accounting for a remarkable 30.2% of the total composition, followed by myrcene (9.5%). This EO was also the one with the highest content of terpinolene among all the EOs, as shown by the GC-FID analysis results. Unlike the previous ones, 24 K, Gorilla Glue, and Pablito EOs were dominated by sesquiterpenes. Among them, (\(E\))-aryophyllene was the most prevalent compound, in line with GC-FID analysis, representing 19.9%, 17.6%, and 15.5% of EO, respectively. It was followed by 10-_epi_-\(\gamma\)-eudesmol (10.2%) and guaiol (9.7%) in 24 K, and by selina-3/1(11)-diene (12.5%) and selina-4(15),7(11)-diene (7.1%) in Gorilla Glue. With respect to the other EOs, Pablito contained a higher relative percentage of \(\alpha\)-bisabolol (8.6%). Regarding cannabinoids, the only compounds detected in the nine commercial varieties' EOs were CBD and cannabichromene (CBC). While CBC was found only in traces in all the EOs, CBD content ranged from 0.1% to 0.6%, except in Venom OG, where it reached 1.0% (Table 2). The above-described GC-MS outcomes highlight a certain variability in the chemical profiles of the investigated EOs, among each other and also, in some cases, with respect to those obtained by the certified industrial hemp varieties which were the relatives of the nine commercial cultivars considered in this study. In fact, the Pablito EO was characterized by the prevalence of (\(E\))-aryophyllene and other sesquiterpenes, while its original monoecious variety Santhica 70 was reported to be richer in monoterpenes, especially myrcene, although its EO was obtained by steam distillation [42]. 24 K and Gorilla Glue, deriving from Carmagnola CS, presented the sesquiterpenes class and, particularly, (\(E\))-aryophyllene as predominant constituents; notably, Carmagnola EO showed a higher amount of monoterpenes (especially myrcene) in the case of steam-distilled fresh inflorescences [43], but a higher sesquiterpene content (notably (\(E\))-caryophyllene) when hydrodistillation of dry inflorescences was carried out [44]. Regarding Lemon Conti Kush, Fresh Mountain, and Amnesia Cookies, originated by the Kompolti variety, a considerable abundance of myrcene and other monoterpenes was detected in their EOs. This was in accordance with some literature findings in which the Kompolti EO from dried inflorescences appeared to be richer in the same compounds, rather than in sesquiterpenes [45]. So, it could be deduced that such differences in the distribution of mono- and sesquiterpenes in hemp EOs seem to be dependent on the extraction method and plant material status. Interestingly, in some EOs from the nine commercial varieties, the presence of several sesquiterpenes structurally different from (\(E\))-caryophyllene was found at noteworthy levels (Figure 1). Among them, 10-_epi_-\(\gamma\)-eudesmol and guaiol in 24 K, selina-3,7(11)-diene and selina-4(15),7(11)-diene in Gorilla Glue, and \(\alpha\)-bisabolol in Pablito could be mentioned as the most representative compounds (Table 2). These sesquiterpenes were not frequent in hemp EOs from other studies. For instance, they were missing in the Kompolti EO analyzed by [PERSON] et al. [46], while in [PERSON] et al. [5], selina-3,7(11)-diene and \(\alpha\)-bisabolol were detected, but in very low amounts (<1%), as for all the other minor sesquiterpenes identified in Carmagnola EO.