## 5 Conclusions  
In this work the potential of green extraction of BACs from wild thyme by theoretical and experimental approach was demonstrated. The results of experimental study confirmed the potential of HVED for extractions of wild thyme bioactive compounds using water and ethanol. Extraction yield of polyphenols and antioxidants was enhanced with longer treatment time, higher ethanol content and higher voltage, and it was higher for treatment with nitrogen than argon. In average, HVED extraction showed 2.03 higher yield of extraction compared to CE under same extraction conditions (ethanol \begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{2}{*}{**Sample**} & **High Voltage Treatment Time** & **Voltage** & **Ethanol Content** & **Stirring** & **Extraction** \\  & **(min)** & **(kV)** & **(\%)** & **(min)** & **Type** \\ \hline TN1 & 3 & 20 & 50 & 0 \\ \hline TN2 & 9 & 20 & 0 & 0 \\ \hline TN3 & 3 & 20 & 0 & 0 \\ \hline TN4 & 3 & 25 & 0 & 0 \\ \hline TN5 & 9 & 25 & 25 & 0 \\ \hline TN6 & 9 & 20 & 25 & 0 \\ \hline TN7 & 9 & 20 & 50 & 0 \\ \hline TN8 & 9 & 25 & 50 & 0 \\ \hline TN9 & 3 & 25 & 25 & 0 \\ \hline TN10 & 9 & 25 & 0 & 0 \\ \hline TN11 & 3 & 25 & 50 & 0 \\ \hline TN12 & 3 & 20 & 25 & 0 \\ \hline TA1 & 3 & 15 & 50 & 0 \\ \hline TA2 & 9 & 15 & 0 & 0 \\ \hline TA3 & 3 & 15 & 0 & 0 \\ \hline TA4 & 3 & 20 & 0 & 0 \\ \hline TA5 & 9 & 20 & 25 & 0 \\ \hline TA6 & 9 & 15 & 25 & 0 \\ \hline TA7 & 9 & 15 & 50 & 0 \\ \hline TA8 & 9 & 20 & 50 & 0 \\ \hline TA9 & 3 & 20 & 25 & 0 \\ \hline TA10 & 9 & 20 & 0 & 0 \\ \hline TA11 & 3 & 20 & 50 & 0 \\ \hline TA12 & 3 & 15 & 25 & 0 \\ \hline \hline \end{tabular}
\end{table} 
Table 9: _Cont._content and time of extraction), and it was 2.07 times higher for nitrogen and 1.99 times higher for extraction with argon. The main phenolic compound found in wild thyme extracts was rosmarinic acid, while the predominant volatile compound was monoteroid phenol carvacrol. Furthermore, experimental results confirmed theoretical results since higher solubility of wild thyme bioactive compounds was found in ethanol than water. However, more accurate results were obtained using COSMO-RS software, compared to HSPs. Wild thyme extracts obtained by HVED are considered as safe, in terms of pesticides and metals levels, and present a high-quality source of valuable BACs for further use in functional food production. HVED was presented as fast and effective nonthermal technology where thermolabile BACs are being preserved and recovered in high concentrations. Furthermore, HVED has a potential to be scaled-up to industrial level to replace less environmentally acceptable conventional extraction methods. Finally, the results from this study encourage further investigation of HVED as a green extraction method and its comparison with other green extraction methods. The following are available online, Table S1: Correlations between TPC, DPPH, FRAP and sum of all measured compounds by UPLC-MS/MS and GC-MS methods, Figure S1: UPLC-MS/MS chromatograms of main compounds from extract 3 T0: a) apigenin) and b) rosmarinic acid, Figure S2: UPLC-MS/MS chromatograms of main compounds from extract TN8: a) apigenin), b) luteolin, c) rosmarinic acid, and d) oleanolic acid, Figure S3: GC-MS chromatograms of extracts: a) 3 TO, b) TN4, c) TA4 and d) chemical formulas of main compounds found in wild thyme extracts. Conceptualization, A.R.J. and M.N. ; Methodology, M.N., A.R.J., G.P. ; Software, F.C. ; Validation, A.R.J. ; Formal Analysis, M.N., D.C.; Investigation, M.N., S.C., L.M., F.C., M.B. ; Resources, A.R.J. ; Data Curation, M.N. and A.R.J. ; Writing--Original Draft Preparation, M.N. ; Writing--Review & Editing, G.P., A.R.J. ; Visualization, M.N. ; Supervision, A.R.J., G.P., G.F.; Project Administration, A.R.J. ; Funding Acquisition, A.R.J. and M.N. All authors have read and agreed to the published version of the manuscript.