## 2 Results and Discussion
### Terpene Organic Compound Profiles of Extracts  
The terpene profiles of extracts obtained through pressurized extraction were analyzed using solid-phase microextraction (SPME) combined with GC/MS. The lowest monoterpene-to-sesquiterpene ratio was observed in extracts obtained using acetone at 25 \({}^{\circ}\)C. Conversely, the highest monoterpene content relative to sesquiterpenes was achieved using a hexane/isopropanol mixture (7:3 \(v/v\)) at \(-\)55 \({}^{\circ}\)C. [PERSON] et al. highlighted in their study that a high extraction efficiency for both cannabinoids and terpenes can be achieved using a combination of polar and nonpolar solvents [29]. This observation aligns with the results obtained in our study (Figure 2). The analysis of the terpene fraction profile in extracts obtained using magnetic stirrer-assisted extraction yielded results analogous to those observed for the previous extraction method. The lowest monoterpene-to-sesquiterpene ratio was identified in extracts obtained with acetone at 25 \({}^{\circ}\)C. Conversely, the highest monoterpene content relative to sesquiterpenes was observed in extracts obtained using the same binary solvent mixture (hexane/isopropanol, 7:3 \(v/v\)) as in the pressurized extraction at a temperature of \(-\)55 \({}^{\circ}\)C (Figure 3). Figure 1: Cannabinoid content of extracts from the Futura 75 cultivar obtained by magnetic stirrer extraction with organic solvents at 25 \({}^{\circ}\)C and \(-\)55 \({}^{\circ}\)C.  
Figure 2: The influence of organic solvent on the efficiency of extraction of terpene compounds during pressure extraction from the Futura 75 hemp variety. In the next stage of this study, the identification of terpene compounds present in the extracts was based on two independent methods: comparing the obtained mass spectra with the NIST 20 (National Institute of Standards and Technology) and FFNSC 3.0 (Flavours and Fragrances of Natural and Synthetic Compounds 3.0) databases and comparing the calculated retention indices' LRIs using a retention index calculator with the values provided in the NIST 20 and FFNSC 3.0 databases. A total of 59 compounds were identified in the extracts obtained via both pressurized extraction and magnetic stirrer-assisted extraction, as presented in Table 2 below. \begin{table}
\begin{tabular}{l c c c} \hline \hline
**Compound** & \(\mathbf{LRI_{exp}}^{1}\) & \(\mathbf{LRI_{in}}^{2}\) &
\begin{tabular}{c} **Content Range** \\ **Min-Max [\%]** \\ \end{tabular} \\ \hline \(\alpha\)-Pinene & 933 & 933 & 0.01–1.16 \\ Benzaldehyde & 960 & 960 & 0.03–0.60 \\ Myrcene & 991 & 991 & 0.33–11.50 \\ \(\alpha\)-Phellandrene & 1005 & 1007 & \(\mathrm{T}^{3}\) \\ Unknown terpene & 1010 & & 0.01–0.82 \\ \(\alpha\)-Terpinene & 1017 & 1018 & 0.03–0.87 \\ \(\alpha\)-Cyrene & 1024 & 1025 & 0.07–10.26 \\ Sylvestrene & 1028 & 1031 & \(\mathrm{T}\) \\ Eucalyptusptol & 1031 & 1032 & 0.01–2.37 \\ \(\gamma\)-Terpinene & 1058 & 1058 & 0.01–6.59 \\ _cis_-Sabinene hydrate & 1067 & 1069 & 0.07–0.45 \\ Unknown terpene & 1072 & & 0.03–0.43 \\ _p_-Cymenene & 1090 & 1093 & 0.05–3.36 \\ _trans_-Sabinene hydrate & 1099 & 1099 & 0.03–1.42 \\ Linalool & 1101 & 1101 & 0.02–1.76 \\ Fenchol & 1114 & 1119 & 0.01–3.16 \\ _trans_-Pinene hydrate & 1122 & 1121 & 0.02–0.88 \\ _trans_-p-Mentha-2,8-diene-1-ol & 1136 & 1140 & 0.03–0.40 \\ _trans_-Pinocarveol & 1139 & 1141 & 0.01–0.56 \\ Ipsdeniol & 1147 & 1146 & 0.01–1.82 \\ Unknown terpene & 1158 & & 0.03–3.69 \\ Borneol & 1166 & 1173 & 0.02–2.37 \\ _p_-Cymen-8-ol & 1186 & 1189 & 2.30–15.23 \\ \(\alpha\)-Terpineol & 1192 & 1195 & 0.12–5.57 \\ Unknown terpene & 1198 & & 0.01–0.26 \\ Citronellol & 1229 & 1232 & 0.01–0.21 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Volatile organic compounds identified in the extract of Futura 75. Figure 3: Influence of selected parameters: type of organic solvent, temperature, and time (10, 20, and 40 min) on the terpen profile in the extracts obtained from Futura 75 by extraction performed by a magnetic stirrer.