## 1 Introduction  
The wound is the disruption of cellular and anatomical continuity of living tissue produced by physical, chemical, electrical, or microbial insults to the tissue [1]. The subcutaneous tissue loses its integrity when wounded and colonized by microbes. These microorganisms involve potential pathogens, which interrupt the wound healing process in the injured tissues [2; 3]. Wound infection is a major factor in the progression of chronicity, delaying wound healing [4].  
The development of wound infection is an ongoing problem for many patients. Infected wounds can cause great distress in terms of associated morbidity and mortality, increased length of hospital admission, delayed wound healing, and increased discomfort, and have long been known to increase healthcare costs significantly, as the exudate levels are relatively higher in infected wounds. Pus and odor may indicate that the patient is pyrexial or has an increased neutrophil count [5].  
Most chronic wound microbiome research is focused on bacteria, but contamination of the wound by fungi needs more elucidation. Ref. [6] classified seventeen fungal species from different wounds, which obstruct the healing of these wounds.  
The yeasts of the genus _Candida_ were the most numerous fungus. In particular, the high relative abundance of fungus within the polymicrobial ecology of wound infections, when present, shows that the fungus can be a major contributor to the biofilm of wounds. In addition, the diversity of fungal genera and species found in and among such wounds is more than previously thought or recorded [7]. The vulnerability to fungal wound infection is also increasing in geographical locations with a warm and humid climates [8].  
The link between microbial aggregates and wound healing has been studied using a number of animal models. In order to imitate wound healing, most research used small rodents. However, the architecture of these species' skin and healing patterns differ significantly from those of humans [9]. Horses have thick, tight skin, similar to humans [10]. In humans and horses, wound edge contraction has a minor role (20-25%) in extremity/limb healing, in contrast to the situation in loose-skinned animals such as rodents [11].  
Horses appear to be valid model animals for wound healing in humans because of their similar skin architecture and the relative role of contraction and epithelialization in healing [12; 13]. Because of their surroundings, horses' wounds have a significant risk of infection, as the infected wounds house a variety of microorganisms. However, these germs can be challenging to identify and not react to antibiotic treatment, resulting in chronic non-healing wounds [14]. Therefore, in the study, we selected some cases of equine wounds for clinical application of the gel and to find a resolution for the problem of equine wounds through the investigation of new wound-healing agents.  
Traditional medicine is widespread throughout the world, using natural medicinal plants. _Solenostemma arghel_ is a desert medicinal plant indigenous to African countries (Egypt, Sudan, Algeria, Libya, and Chad) [15], which is considered folk medicine. African people use this plant as an anti-inflammatory, anti-rheumatic, antispasmodic, anti-diabetic, anti-nutrition, antimicrobial, and anticancer agent [16; 17; 18; 19]. The medical effect of different parts of this plant comes especially from leaves because of phytochemicals richness, including various phenolic acids, flavones, glycosylated flavonoids, polyphenols, b-carotene, b-sitosterol, monoterpenes, pregnenes, and pregnane [17; 20].  
The present study was designed to compare the antifungal, antioxidant, and wound healing activities of EtOAc and MeoH _S. arghel_ extracts in albino rats besides the clinical application of the extracts for investigation of wound healing in equines.