## 1 Introduction  
The extraction of bioactive compounds from plant species is one of the oldest and most established applications of biodiversity [1; 2]. The richness of Brazilian flora and fauna, coupled with the severe as a driving force in the search for new bioactive substances with diverse applications, such as compounds in cosmetics, flavors, fragrances, dyes and pigments, general food additives, components in insecticides and pesticides [3], or as important ingredients in various formulations for the treatment of infectious diseases [4]. Natural products derived from plants have served as medicine, given the broad efficiency of bioactive compounds against infectious agents [1; 5]. The use of therapeutic agents based on plant derivatives is justified by the advancement of scientific knowledge in this area [6]. The lack of accessibility, the inefficacy of available drugs, and the high cost of antibiotics, especially as the occurrence of multidrug-resistant bacteria becomes increasingly common [7; 8], are significant challenges. A large part of the therapeutic action of plants is related to the bioactive properties of secondary compounds such as polyphenols, flavonoids, phenolic acids, terpenoids, saponins, alkaloids and others [3; 5; 7; 9]. The Malpighiaceae, which is part of the Cerrado biome, one of the largest in Brazil [10], is composed of 45 genera, includes the genus _Banisteriopsis_, considered a sister genus to the species of interest in this investigation: _Diplopterys pubipetala_ ([PERSON].) [PERSON] & [PERSON], commonly known as \"marvaquero\" (in northern Minas Gerais, Brazil), \"cipo preto\" [11], or \"tucunaca\" [12]. Regarding the species _D. pubipetala_, there are documented findings related to the botanical, anatomical, and physiological aspects of this liana [13; 14; 15; 16; 17]. Other areas of interest have gained significance, including the diversity of secondary metabolites revealed through phytochemical and histochemical tests, as well as the quantification of phenolic compounds and flavonoids, which showed values comparable to or even better than those of biologically significant substances previously quantified in the family [18; 19]. In an _in vitro_ analysis of anticancer activity, the ethyl acetate fraction from leaves and stems reduced the viability of B16F10 cells by over 50% at a concentration of 125 mg.mL\({}^{-1}\), whereas other species widely used as chemotherapeutic agents failed to achieve such reduction in different cancer cell lines at this concentration [20]. Additionally, in tests of anti-inflammatory potential using the dichloromethane and ethyl acetate fractions on 3T3 mouse fibroblast cells exposed to ethyl acetate fractions, there was a decrease in the production of pro-inflammatory cytokines (TNF-\(\alpha\) and IL-6) and an increase in the production of interleukin-10 and nitric oxide levels. These findings indicate the anti-inflammatory and immunoregulatory actions that may be exerted by the explored fraction [21]. In a study conducted on seeds, chromatographic analysis confirmed the presence of unsaturated fatty acids such as oleic, linoleic, and eicosanoic acids, which, similar to other species in the family, are already exploited by the industry. Furthermore, the species was reported as having promising potential in the oilseed sector due to the high content of these substances extracted from the seeds [22]. _Banisteriopsis_ and other genera belonging to the Cerrado biome, with more advanced studies based on biomonitoring [23; 24; 25; 26], stand out through chromatographic and spectroscopic analyses. Through ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS), secondary metabolites have been identified in stem and leaf extracts of _D. pubipetala_, with the presence of alkaloids, flavonoids, terpenoids, saponins, and lactones documented in the literature [18; 19]. However, there are still gaps, both in the study of non-volatile substances and in the investigation of possible volatile substances present in this species.