## 2 Results and Discussion
### GCMS Analysis of Non-Volatile Primary Metabolites Post Silylation
** & \(R_{t}\) **(min)** & **RI** & **Compound** & **RW** & **RS** & **P\({}_{1}\)** & **P\({}_{2}\)** & **C** \\ \hline Total amino acids & & & & 0.46 & 0.60 & 0.73 & 0.96 & 11.01 \\ Fatty acids & & & & & & & \\ \hline S28 & 13.81 & 1653.8 & Lauric acid, TMS & & 2.37 \(\pm\) 0.65 & 7.58 \(\pm\) 1.21 & 4.53 \(\pm\) 3.85 & 7.62 \(\pm\) 1.75 & 0.02 \(\pm\) 2.97 \\ S29 & 15.95 & 1850.2 & Myristic acid, TMS & & 2.23 \(\pm\) 0.59 & 5.39 \(\pm\) 1.16 & 3.50 \(\pm\) 2.62 & 5.89 \(\pm\) 1.18 & 0.04 \(\pm\) 2.41 \\ S30 & 17.90 & 2045.1 & Palmitic acid, TMS & & 3.45 \(\pm\) 0.89 & 7.70 \(\pm\) 1.81 & 6.41 \(\pm\) 3.70 & 9.49 \(\pm\) 2.17 & 0.60 \(\pm\) 3.86 \\ S31 & 19.49 & 2205.5 & Oice acid, TMS & & 3.74 \(\pm\) 1.17 & 11.51 \(\pm\) 1.82 & 7.09 \(\pm\) 5.99 & 14.28 \(\pm\) 2.75 & 0.08 \(\pm\) 5.98 \\ S32 & 19.69 & 2226.5 & Stearic acid, TMS & & 1.28 \(\pm\) 0.43 & 3.32 \(\pm\) 0.60 & 2.14 \(\pm\) 1.62 & 4.20 \(\pm\) 0.79 & 0.19 \(\pm\) 1.78 \\ S33 & 21.36 & 2394.5 & Eicosanoic acid, TMS & & 6.59 \(\pm\) 2.05 & 15.26 \(\pm\) 3.21 & 16.62 \(\pm\) 3.72 & 17.06 \(\pm\) 6.87 & 4.31 \(\pm\) 5.76 \\ Total fatty acids & & & & 19.64 & 50.77 & 4.001 & 58.54 & 5.24 \\ Sugars & & & & & & & \\ \hline S34 & 11.97 & 1499.6 & L-Threitol, tetra-TMS & 0.38 \(\pm\) 0.15 & 0.14 \(\pm\) 0.16 & 0.29 \(\pm\) 0.01 & 0.25 \(\pm\) 0.14 & 1.26 \(\pm\) 0.12 \\ S35 & 12.21 & 1518.9 & 2-Deoxy-D-ribose, tri-TMS & 0.01 \(\pm\) 0.00 & 0.03 \(\pm\) 0.01 & 0.02 \(\pm\) 0.01 & 0.05 \(\pm\) 0.02 \\ S36 & 13.56 & 1632.7 & Kibertonarase, tetra-TMS & & 0.01 \(\pm\) 0.00 & 0.01 \(\pm\) 0.01 & 0.03 \(\pm\) 0.01 & 3.24 \(\pm\) 0.01 \\ S37 & 13.62 & 1637.3 & Arabidopsisynynorez, tetra-TMS & & - & 0.01 \(\pm\) 0.00 & 0.01 \(\pm\) 0.00 & 0.00 \(\pm\) 0.00 & 0.59 \(\pm\) 0.01 \\ S38 & 13.98 & 1669.2 & Kiboryanose, tetra-TMS & & 0.01 \(\pm\) 0.01 & 0.01 \(\pm\) 0.00 & 0.01 \(\pm\) 0.00 & 0.01 \(\pm\) 0.00 & 3.61 \(\pm\) 0.01 \\ S39 & 14.30 & 1669.5 & Kibertonarase, tetra-TMS isomer & 0.36 \(\pm\) 0.21 & 0.11 \(\pm\) 0.10 & 0.09 \(\pm\) 0.06 & 0.10 \(\pm\) 0.02 & 0.43 \(\pm\) 0.04 \\ S40 & 15.21 & 1779.0 & Sorburdinov, penta-TMS & & 0.35 \(\pm\) 0.43 & 0.01 \(\pm\) 0.07 & 0.01 \(\pm\) 0.23 & 0.11 \(\pm\) 0.57 \\ S41 & 15.36 & 1794.1 & Circutorange, penta-TMS & & 1.55 \(\pm\) 0.75 & 0.28 \(\pm\) 0.57 & 0.10 \(\pm\) 0.24 & 0.21 \(\pm\) 0.24 & 1.54 \(\pm\) 0.02 \\ S42 & 15.41 & 1798.6 & Steloyanose, penta-TMS & & 0.03 \(\pm\) 0.02 & 0.01 \(\pm\) 0.01 & 0.00 \(\pm\) 0.00 & 1.11 \(\pm\) 0.01 \\ S43 & 15.49 & 1806.1 & Tagdatramose, penta-TMS & & 0.46 \(\pm\) 0.28 & 0.02 \(\pm\) 0.12 & 0.02 \(\pm\) 0.13 & 0.02 \(\pm\) 0.06 & 0.13 \(\pm\) 0.06 \\ S44 & 15.65 & 1821.7 & Taidoranose, penta-TMS & & 6.73 \(\pm\) 2.96 & 0.18 \(\pm\) 2.66 & 0.14 \(\pm\) 1.53 & 0.09 \(\pm\) 1.31 & 0.28 \(\pm\) 0.78 \\ S45 & 15.79 & 1835.4 & Pistolarase, penta-TMS & & 5.68 \(\pm\) 2.39 & 0.21 \(\pm\) 2.33 & 0.30 \(\pm\) 1.24 & 0.27 \(\pm\) 1.02 & 1.77 \(\pm\) 0.51 \\ S46 & 15.81 & 1887.3 & Taloyranose, penta-TMS & & 1.30 \(\pm\) 0.30 & 0.01 \(\pm\) 0.71 & 0.01 \(\pm\) 0.35 & 0.02 \(\pm\) 0.35 & 2.40 \(\pm\) 0.19 \\ S47 & 15.87 & 1843.2 & Taidoranose, penta-TMS isomer & & 5.01 \(\pm\) 3.29 & 0.03 \(\pm\) 1.21 & 0.11 \(\pm\) 1.65 & 0.03 \(\pm\) 0.85 & 1.02 \(\pm\) 0.81 \\ S48 & 16.12 & 1867.1 & Galactoryanose, penta-TMS & & 3.35 \(\pm\) 1.25 & 0.06 \(\pm\) 1.01 & 0.082 \(\pm\) 0.00 & 0.08 \(\pm\) 0.46 \\ S49 & 16.30 & 1884.0 & 3-Deoxy-arshone-hexonic acid, penta-TMS & & 4.90 \(\pm\) 1.60 & 0.26 \(\pm\) 2.33 & 0.26 \(\pm\) 1.05 & 0.24 \(\pm\) 1.04 & 5.49 \(\pm\) 0.47 \\ S50 & 16.66 & 1692.0 & 1535.6 & penta-PR, TMS-oxy serine & & 0.57 \(\pm\) 0.56 & 0.00 \(\pm\) 0.01 & 0.00 \(\pm\) 0.32 & 0.00 \(\pm\) 0.18 \\ S51 & 16.69 & 1922.0 & Circutonic acid -lactone TMS, trimethyloxyovazine & & 0.60 \(\pm\) 0.03 & 0.01 \(\pm\) 0.02 & 0.01 \(\pm\) 0.01 & 0.01 \(\pm\) 0.01 & 0.27 \(\pm\) 0.00 \\ S52 & 16.81 & 1934.9 & Glucose, penta-TMS & & 1.23 \(\pm\) 0.60 & 0.01 \(\pm\) 0.45 & 0.00 \(\pm\) 0.31 & 0.00 \(\pm\) 0.23 & 0.02 \(\pm\) 0.16 \\ S53 & 17.03 & 1997.5 & Colonic acid, -14,lactone, tetra-TMS & & 1.32 \(\pm\) 0.82 & 0.10 \(\pm\) 0.35 & 0.01 \(\pm\) 0.37 & 0.05 \(\pm\) 0.20 & 0.21 \(\pm\) 0.16 \\ S54 & 17.23 & 1977.1 & Minnitol, hexa-TMS & & 0.32 \(\pm\) 0.25 & 0.08 \(\pm\) 0.05 &