## 5 Conclusions  
In conclusion, the bioengineering of _P. tricornutum_ with cannabis genes represents a significant advancement in CB production technology. This innovative approach not only provides a sustainable alternative to traditional cannabis cultivation but also highlights the potential of marine microorganisms as versatile bioreactors for valuable natural products. As synthetic biology and metabolic engineering continue to evolve, the intersection of marine biology and cannabis biosynthesis could shape the future of CB production.  
**Author Contributions**: [PERSON], [PERSON], [PERSON], F.M.-M. and [PERSON] contributed to the study's conception and design. Methodology, material preparation, data collection, and analysis were performed by [PERSON], [PERSON], [PERSON], A.M.D.-G., [PERSON], [PERSON], [PERSON] and [PERSON]-M. Episomal constructions, diatom conjugation, and molecular biology experiments were planned and conducted by [PERSON], [PERSON], [PERSON], [PERSON], [PERSON], [PERSON], [PERSON] and [PERSON]. HPLC analyses were performed by [PERSON], [PERSON] and [PERSON], whereas all LC-MS analyses were performed by [PERSON]. The first draft of the manuscript was written by [PERSON], [PERSON], [PERSON] and [PERSON]. [PERSON] and [PERSON] edited the previous versions of the manuscript. [PERSON] supervised the project and secured the funding. All authors have read and agreed to the published version of the manuscript.  
**Funding:** This research was funded by the Natural Sciences and Engineering Research Council of Canada through the Alliance Program (award nos. ALLRP 554429-20 and ALLRP 570476-2021) to [PERSON]. Additional support in the form of scholarships was awarded to F.A. from MITACS-Elevation IT15971, to E.I.F. and A.M.D.-G. from the MITACS-Acceleration program (grant nos. IT12310, IT16463, and IT19432), and to [PERSON] is also acknowledged. B.J.K. is supported by Natural Sciences and Engineering Research Council of Canada (RGPIN-2018-06172).  
**Data Availability Statement**: Data is contained within the article.  
**Acknowledgments**: The authors would like to acknowledge [PERSON] from the Centre National en Electrochimie et en Technologies Environnementales Inc. for LC-MS analyses, and [PERSON], [PERSON], and [PERSON] from the University du Quebec a Trois-Rivieres for their guidance, mentoring, and assistance.  
**Conflicts of Interest**: The authors declare that this study received funding from Algae-C. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication.  
**Abbreviations**  
\begin{tabular}{l l} Au & Absorbance units \\ CB & Cannabinoid \\ CBD & Cannabidiol \\ CBDA & Cannabidiolic acid \\ CBGA & Cannabigerolic acid \\ cPCR & Colony polymerase chain reaction \\ FACS & Fluorescence-activated cell sorting \\ Gly & Glycine \\ GPP & Geranyl pyrophosphate \\ His & Histidine \\ OA & Olivetolic acid \\ OAC & Olivetolic acid \\ OD & Optical density \\ Ser & Serine \\ T2A & _Thosea asigma_ virus self-cleavable sequence \\ THC & \(\Delta\)-9-tetrahydrorocannabinol \\ THCA & Delta (9)-tetrahydrorocannabinolic acid \\ TKS & Tetaketide synthase \\ YFP & Yellow fluorescent protein \\ \end{tabular}