## 2 Materials and Methods
### Chemistry
\(N^{\prime}\)-[(\(E\))-(2-bromo-3-hydroxy-4-methoxyphenyl)methylidene]-2-phenylacetohydrazide (**9**)  
Color: white; M.p. : 184-186 \({}^{\circ}\)C; yield: 61%; for C\({}_{16}\)H\({}_{15}\)BrN\({}_{2}\)O\({}_{3}\) (363.21) calculated: C: 52.91%, H: 4.16%, N: 7.71%; found: C: 52.98%, H: 4.14%, N: 7.69%. IR (cm\({}^{-1}\)): 3678 (OH), 3254 (NH), 3064 (CH, arom. ), 2941 (CH, aliph. ), 1652 (C=O), 1593 (C=N); \({}^{1}\)H NMR (600 MHz, DMSO-\(d_{6}\)): 3.53 (s, 2H, CH\({}_{2}\)), 3.88 (s, 3H, OCH\({}_{3}\)), 7.08 (t, 1H, ArH, \(J\) = 6 Hz), 7.21-7.27 (m, 2H, ArH), 7.30-7.35 (m, 3H, ArH), 7.41 (d, 1H, ArH, \(J\) = 6 Hz), 7.46 (d, 1H, ArH, \(J\) = 6 Hz), 8.32 (s, 1H, =CH), 11.45 (s, 1H, OH), 11.72 (s, 1H, NH); \({}^{13}\)C NMR (150 MHz, DMSO-\(d_{6}\)): 41.74 (CH\({}_{2}\)), 56.74 (OCH\({}_{3}\)), 111.58, 112.12, 117.83, 126.84, 128.70, 129.83, 136.22, 142.65, 144.22, 146.05 (12C\({}_{\rm ar}\)), 149.79 (=CH), 166.88 (C=O). \(N^{\prime}\)-[(\(E\))-(4-hydroxy-3-iodo-5-methoxyphenyl)methylidene]-2-phenylacetohydrazide (**10**)  
Color: cream; M.p. : 210-214 \({}^{\circ}\)C; yield: 71%; for C\({}_{16}\)H\({}_{15}\)IN\({}_{2}\)O\({}_{3}\) (410.21) calculated: C: 46.85%, H: 3.69%, N: 6.83%; found: C: 46.98%, H: 3.70%, N: 6.85%. IR (cm\({}^{-1}\)): 3628 (OH), 3182 (NH), 3056 (CH, arom. ), 2999, 2965 (CH, aliph. ), 1643 (C=O), 1606 (C=N); \({}^{1}\)H NMR (600 MHz, DMSO-\(d_{6}\)): 3.54 (s, 2H, CH\({}_{3}\)), 3.88 (s, 3H, OCH\({}_{3}\)), 7.21-7.27 (m, 2H, ArH), 7.28-7.34 (m, 3H, ArH), 7.54 (d, 1H, ArH, \(J\) = 18 Hz), 7.83 (s, 1H, ArH), 8.06 (s, 1H, =CH), 11.32 (s, 1H, OH), 11.55 (s, 1H, NH); \({}^{13}\)C NMR (150 MHz, DMSO-\(d_{6}\)): 41.66 (CH\({}_{2}\)), 56.56 (OCH\({}_{3}\)), 84.89, 109.53, 126.80, 128.68, 129.48, 129.81, 130.55, 136.38, 142.06, 145.84 (12C\({}_{\rm ar}\)), 147.72 (=CH), 166.84 (C=O). \(N^{\prime}\)-[(\(E\))-(3-ethoxy-2-hydroxyphenyl)methylidene]-2-phenylacetohydrazide (**11**)  
Color: cream; M.p. : 134-136 \({}^{\circ}\)C; yield: 83%; for C\({}_{17}\)H\({}_{18}\)N\({}_{2}\)O\({}_{3}\) (298.34) calculated: C: C: 68.44%, H: 6.08%, N: 9.39%; found: C: 68.37%, H: 6.10%, N: 9.41%. IR (cm\({}^{-1}\)): 3307 (OH), 3181 (NH), 3083 (CH, arom. ), 2969 (CH, aliph. ), 1659 (C=O), 1604 (C=N); \({}^{1}\)H NMR (600 MHz, DMSO-\(d_{6}\)): 1.34 (t, 3H, CH\({}_{3}\)), \(J\) = 6 Hz), 3.57 (s, 2H, CH\({}_{2}\)), 4.05 (q, 2H, CH\({}_{2}\), \(J\) = 6 Hz), 6.80-6.84 (m, 1H, ArH), 6.97-7.01 (m, 1H, ArH), 7.10 (d, 1H ArH, \(J\) = 6 Hz), 7.21-7.28 (m, 2H, ArH), 7.30-7.35 (m, 3H, ArH), 8.42 (s, 1H, =CH), 11.37 (s, 1H, OH), 11.84 (s, 1H, NH); \({}^{13}\)C NMR (150 MHz, DMSO-\(d_{6}\)): 15.21 (CH\({}_{3}\)), 41.45 (CH\({}_{2}\)), 64.59 (OCH\({}_{2}\)), 115.63, 119.47, 121.35, 127.13, 128.70, 128.83, 129.59, 129.83, 135.91, 141.40 (12C\({}_{\rm ar}\)), 147.50 (=CH), 166.79 (C=O). \(N^{\prime}\)-[(\(E\))-(2-hydroxy-3-nitrophenyl)methylidene]-2-phenylacetohydrazide (**12**)  
Color: orange; M.p. : 176 \({}^{\circ}\)C; yield: 67%; for C\({}_{15}\)H\({}_{13}\)N\({}_{3}\)O\({}_{4}\) (299.28) calculated: C: 60.20%, H: 4.38%, N: 14.04%; found: C: 60.11%, H: 4.39%, N: 14.08%. IR (cm\({}^{-1}\)): 3484 (OH), 3177, 3107 (NH), 3077 (CH, arom. ), 2948 (CH, aliph. ), 1663 (C=O), 1614 (C=N); \({}^{1}\)H NMR (600 MHz, DMSO-\(d_{6}\)): 3.62 (s, 2H, CH\({}_{2}\)), 7.10-7.15 (m, 1H, ArH), 7.23-7.28 (m, 1H, ArH), 7.28-7.36 (m, 5H, ArH), 7.89-7.99 (m, 1H, ArH), 8.50 (s, 1H, =CH), 11.61 (s, 1H, OH), 12.17 (s, 1H, NH); \({}^{13}\)C NMR (150 MHz, DMSO-\(d_{6}\)): 41.30 (CH\({}_{2}\)), 119.62, 12=CH), 8.54-8.57 (m, 1H, ArH), 11.54 (s, 1H, OH), 12.03 (s, 1H, NH); \({}^{13}\)C NMR (150 MHz, DMSO-\(d_{6}\)): 41.45 (CH\({}_{2}\)), 117.55, 121.87, 124.27, 127.17, 128.84, 129.58, 135.78, 140.36 (10\(\mathrm{C}\mathrm{a}\mathrm{r}\)), 143.76 (=CH), 162.25, 162.97 (2\(\mathrm{C}\mathrm{a}\mathrm{r}\)), 167.23 (C=O). N\({}^{\prime}\)-[(E)-(4-hydroxy-3-nitrophenyl)]methylidene]-2-phenylacetohydrazide **(14)**  
Color: orange; M.p.