## 4 Discussion  
The results of lettuce seeds germination, after treatment with aqueous extracts of chia, black cumin, wormwood herbs, and nettle (Table 7), as well as hypocotyl and radicle length of lettuce seedlings (Tables 10 and 13), partially correspond with the results of previous studies [24; 25]. The authors of previous studies found an inhibitory effects of epicatechin on the growth of lettuce seedlings. However, in the case when epicatechin is applied with hydroquinone, the epicatechin tends to counteract the growth inhibition activity of hydroquinone [26]. Moreover, the application of chia aqueous extracts in all concentrations, i.e., of 2.5, 5 and 10%, respectively, significantly increased the lettuce seeds germination in comparison with the control (Table 7). On the other hand, only one variant of spinach seeds treated with aqueous extract of chia saw a significant increased in the spinach \begin{table}
\begin{tabular}{c c c c c} \hline \hline \multicolumn{2}{c}{**Control**} & \multicolumn{3}{c}{**1.77 \(\pm\) 0.34**} \\ \multicolumn{2}{c}{**(Not Treated)**} & & **Black Cumin** & **Wormwood Herbs** & **Nettle** \\  & **(S. _higpanica_ L.)** & **(N. _sativa_ L.)** & **(_A. absinthium_ L.)** & **(_U. & _dioica_ L.)** \\ \hline
2.5\% & 2.92 \(\pm\) 0.67 \({}^{\text{a}}\) & 3.37 \(\pm\) 0.22 \({}^{\text{a}}\) & 3.05 \(\pm\) 0.45 \({}^{\text{n.s}}\) & 1.67 \(\pm\) 0.30 \({}^{\text{a}}\) \\
5\% & 2.98 \(\pm\) 1.08 \({}^{\text{b}}\) & 2.47 \(\pm\) 0.59 \({}^{\text{b}}\) & 2.35 \(\pm\) 0.31 \({}^{\text{n.s}}\) & 0.57 \(\pm\) 0.40 \({}^{\text{b}}\) \\
10\% & 2.25 \(\pm\) 0.34 \({}^{\text{b}}\) & 1.77 \(\pm\) 0.15 \({}^{\text{b}}\) & 1.30 \(\pm\) 0.01 \({}^{\text{n.s}}\) & 0.0 \(\pm\) 0.00 \\ \hline \hline \end{tabular} The values with different letters in superscript are significant \(p\) \(<\) 0.05; (n.s. = not significant).  
\end{table} 
Table 13: Comparison of differences of means for radicle length of lettuce (_L. sativa_ L.) seedlings (The values in the table represent the mean value of radicle length in cm \(\pm\) SD). \begin{table}
\begin{tabular}{c c c c c} \hline \hline \multicolumn{2}{c}{**Control**} & \multicolumn{3}{c}{**2.55 \(\pm\) 0.24**} \\ \multicolumn{2}{c}{**(Not Treated)**} & & **Black Cumin** & **Wormwood Herbs** & **Nettle** \\  & **(S. _higpanica_ L.)** & **(N. _sativa_ L.)** & **(_A. absinthium_ L.)** & **(_U. & _dioica_ L.)** \\ \hline
2.5\% & 2.30 \(\pm\) 0.43 \({}^{\text{n.s.}}\) & 2.42 \(\pm\) 0.26 \({}^{\text{a}}\) & 2.27 \(\pm\) 0.67 \({}^{\text{a}}\) & 2.25 \(\pm\) 0.30 \({}^{\text{a}}\) \\
5\% & 2.17 \(\pm\) 0.39 \({}^{\text{n.s.}}\) & 1.65 \(\pm\) 0.13 \({}^{\text{b}}\) & 2.5 \(\pm\) 0.14 \({}^{\text{a}}\) & 1.15 \(\pm\) 0.38 \({}^{\text{b}}\) \\
10\% & 2.15 \(\pm\) 0.24 \({}^{\text{n.s.}}\) & 1.32 \(\pm\) 0.57 \({}^{\text{c}}\) & 1.55 \(\pm\) 0.49 \({}^{\text{b}}\) & 0.0 \(\pm\) 0.00 \\ \hline \hline \end{tabular} The values with different letters in superscript are significant \(p\) \(<\) 0.05; (n.s. = not significant).  
\end{table} 
Table 11: Comparison of differences of means radicle length of pepper (_C. annuum_ L.) seedlings (The values in the table represent the mean value of radicle length in cm \(\pm\) SD). seeds germination (Table 6), although all the treatments with all aqueous extracts in all concentrations significantly decreased the pepper seeds germination (Table 5). Additionally, when comparing these results with the results of hypocotyl and radiicle lengths of pepper seedlings in the same variants of trial, it is visible that the decrease in hypocotyl (Table 8) and radiicle length (Table 11) corresponds with decrease in the germination of pepper seeds (Table 5). The correspondence between the results of lettuce seeds germination and lengths of lettuce seedlings hypocotyl and radiicle also occurs (Tables 7, 10 and 13), but there is no correspondence between the spinach seeds germination and lengths of hypocotyl and radiicle of spinach seedlings (Tables 6, 9 and 12).