## 3 Results
### Test on OECD Soils
#### 3.1.2 Cucurbita pepo  
Assay  
The in vivo assay on _C. pepo_ was performed on the same soils from the in vitro assay. Due to technical difficulties inherent to the experimental design (weak soil quantity in each pot), some repetition did not grow properly. Thus, 4 repetitions were obtained from BC2 and BC3 groups, whereas only 1 repetition was obtained from AC3 group instead of the 5 repetitions sown for each modality. As the characteristics of the AC amendment could also strongly influence nutrient availability and water retention, they can interfere  
Figure 1: Environmental available concentrations of polychlorobiphenyls (PCB) PCB 101, PCB 138, PCB 153 and PCB 180 present in artificial soil. Values correspond to the mean \(\pm\) Standard Error (SE) (n = 3) concentrations of (**A**) PCB 101, (**B**) PCB 138, (**C**) PCB 153 and (**D**) PCB 180 obtained through environmental availability assay. Group mean values with superscript asterisks are statistically different from non-amended but contaminated Standard Soil (SS) (0.05: * > 0.01: ** > 0.001: ***) using a variance analysis and a Dunnett post-hoc test. Controls, representing soil without any spiking of the pollutants, were not included in the tested dataset. Groups mean concentrations with different superscript letters (a, b) are statistically different (\(p<0.05\)) from each other using a complementary variance analysis followed by a [PERSON] post-hoc test on previously found significant groups using Dunnett’s. #: Values below Limits of Quantitation (LOQs) were replaced by the corresponding LOQ, superscript numbers indicate the number of replaced values.  
with plant development. Considering transfer, and similarly to previous environmental availability results, BCs and AC1 were the less effective materials. Results were even more contrasted as for all studied NDL-PCBs no significant reductions were found for these materials compared to the SS control group (Figure 2). At the opposite, AC2 and AC3 were also the most effective matrices and interestingly, levels of reduction found were greater than previous measurements as the analytical quantitation limits were far lower than concentrations found in the non-amended control group. Thus, the minimal reduction factors which could be derived from this assay were the following: 93% for PCB 101, 95% for PCB 138, 95% for PCB 153 and 92% for PCB 180. Thus, from this in vivo assay, the following efficiency hierarchy was found between the used sequestration materials: BC1 = BC2 = BC3 = AC1 << AC2 = AC3.  
Figure 2: In vivo concentrations of PCB 101, PCB 138, PCB 153 and PCB 180 in aerial parts of _Cucurbita pepo_. Values correspond to the mean \(\pm\) SE (n = 5) concentrations of **(A)** PCB 101, **(B)** PCB 138, **(C)** PCB 153 and **(D)** PCB 180 obtained through the in vivo _Cucurbita pepo_ assay. Group mean values with superscript asterisks are statistically different from non-amended soil (SS) (0.05: * > 0.01: **> 0.001: ***) using a variance analysis and a Dunnett post-hoc test. Controls were not included in the tested dataset. Groups mean concentrations with different superscript letter (a, b) are statistically different (\(p<0.05\)) from each other using a complementary variance analysis followed by a Tukey post-hoc test on previously found significant groups using Dunnett’s. #: Values below LOQs were replaced by the corresponding LOQ, superscript numbers indicate the number of replaced values. DM stands for Dry Matter.