Previously, to overcome these problems, the management of water systems to control environmental factors from climate change and the prophylactic use of antibiotics to prevent disease outbreaks were explored. However, the water management system results in high costs for the farming industry, while the use of antibiotics is not sustainable and environmentally-friendly and prohibited in many countries. Biofloc technology presents a promising alternative to managing water systems and could potentially reduce the risk of diseases to counter the negative side of intensive closed culture systems [11]. Biofloc is an aggregate of microorganism--bacteria, microalgae and protozoa--and organic matter that has high porosity [12]. Water with high nitrogen (N) and phosphorus (P) compounds can pass through the pores in biofloc, allowing the nutrient exchange. Biofloc can reduce the external organic input by microbial activities and convert it into biomass, which can be used as a natural feed source for shrimp. This natural feed can decrease the use of commercial feed in the intensive culture system, thereby reducing the production cost [12]. Benefits of biofoc technology have been shown to improve water quality [13; 14; 15], increase growth in shrimp culture [16; 17], protect against pathogenic _Vibrio_[18; 19; 20] and provide supplementary nutritional intake to many aquatic animals [12; 21; 22]. Generally, one of the microbial communities in biofloc, genera of _Bacillus_, also has the potency to help in the digestion and absorption process in the gut of shrimp by breaking down the larger particles into smaller ones and results in better growth [19]. Nonetheless, the most implemented biofoc technology is in situ biofoc which is naturally formed in the water column. The weakness of the in-situ biofloc is the unknown composition and function of the biofoc. Therefore, the production and application of ex-situ biofoc are needed in order to optimize the performance of biofoc technology with the possibility to predict and customize the function of the biofoc. The ex-situ biofoc can be produced in suspended growth bioreactors, and the dried (powdered) biofoc can be used as a feed supplement to improve growth performance, immune responses, and metabolic activities of shrimp such as _Peneus monodon_[23; 24] and _Litopenaeus vannamei_[25]. Previously, we established ex-situ biofoc containing probiotic bacteria (_B. cereus_, _B. megaterium_), microalgae (_Chaetoceros calcitrans_), and a consortium of nitrifying bacteria according to their beneficial effects as aforementioned. The ex-situ biofoc using an inoculum percentage (% \(v/v\)) (_B. megaterium_:_B. cereus_:_C. calcitrans_: Nitrifying bacteria) of 1:1:6:9 was found to be an optimal ratio based on biofoc porosity and volume (unpublished data). Despite its benefits, there is still limited knowledge on how biofoc technology affects the shrimp growth performance and enhances shrimp nutritional value for food security, especially at the molecular levels. Therefore, in this study, we aim to evaluate the effects of the established ex-situ biofoc supplementation in Pacific white shrimp (_L. vannamei_) cultured on water quality management, the shrimp growth performance and nutritional properties, and growth-related genes to shed light on the molecular mechanism of the benefits of the ex-situ biofloc on shrimp.