## 3 Results
### Microbiological Stability of Creans with Eugenol and Isoeugenol  
The results in Table 5 present the activity of eugenol and isoeugenol at different concentrations on the growth of bacteria and yeast-like fungi in the cream samples. The creans containing eugenol at the concentrations of 0.5%, 1.5%, and 2.5%, and isoeugenol at the same concentrations, were tested on PCA, MC, MSA, BHI broth, and D/E neutralizing broth. No growth of bacteria or yeast-like fungi was observed in any of the cases. These results suggest that eugenol and isoeugenol can effectively reduce the growth and/or eliminate microorganisms from the cream samples, which is important for maintaining the hygiene and safety of cosmeceutical products. The antimicrobial activity of eugenol and isoeugenol was widely studied and well documented. Their main mechanism of action is the ability to disrupt the integrity of the cell membranes of microorganisms, leading to their damage and death. In addition, these substances show the ability to inhibit the activity of enzymes that are essential for bacterial life and function [6; 7; 9; 10; 11]. In the case of yeast-like fungi, the action of eugenol and isoeugenol also involves interfering with the metabolic processes necessary for their growth and reproduction. Yeast-like fungi, like bacteria, have their own unique metabolic pathways that are crucial to  
Figure 4: Sample photos of the results of the disk diffusion method for yeast-like fungi. \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Time After** & **Concentration** & **Concentration** & **Medium (Type of** **Microorganism)** & **Test Result** \\ \hline \multirow{3}{*}{1 day} & 0.50\% & 0.50\% & \multirow{3}{*}{PCA, MC, MSA, \(2.5\%\)} & No microbial \\  & 1.50\% & 1.50\% & & BHI, D/E \\  & 2.50\% & 2.50\% & & \\ \hline \multirow{3}{*}{1 week} & 0.50\% & 0.50\% & \multirow{3}{*}{PCA, MC, MSA, \(2.5\%\)} & No microbial \\  & 1.50\% & 1.50\% & & BHI, D/E \\  & 2.50\% & 2.50\% & & \\ \hline \multirow{3}{*}{1 month} & 0.50\% & 0.50\% & \multirow{3}{*}{PCA, MC, MSA, \(2.5\%\)} & No microbial \\  & 1.50\% & 1.50\% & & BHI, D/E \\ \cline{1-1} \cline{3-5}  & 0.50\% & 0.50\% & & \\ \cline{1-1} \cline{3-5}  & 1.50\% & 1.50\% & & \\ \cline{1-1} \cline{3-5}  & 2.50\% & 2.50\% & & \\ \hline \end{tabular} Legend: PCA—agar medium for general microorganisms; MC—MacConkey medium (_Gram-negative bacteria_); MSA—selective agar for _Staphylococcus_ spp_; BHI—Brain Heart infusion (general bacterial medium). D/E neutralizing broth—neutralizing medium to detect the presence of bacteria.  
\end{table} 
Table 5: Results of microbiological stability tests of creans containing eugenol and isoeugenol. their survival. Eugenol and isoeugenol can interfere with these processes, leading to the inhibition of fungal growth and proliferation [19; 20; 40; 41]. One important point to emphasize is the selectivity of eugenol and isoeugenol. This means that these substances can be effective in the elimination of harmful microorganisms, but at the same time can have minimal or no effect on beneficial microorganisms, such as the probiotic gut bacteria. This property is particularly important in the context of antimicrobial and antifungal therapy, as it allows the selective destruction of pathogens while minimizing the disruption of the body's natural microbiotic flora [6; 13; 14; 17]. In addition, studies on the synergistic effects of eugenol and isoeugenol with other substances or drugs may bring new therapeutic possibilities. Combinations of these compounds with the existing antibiotics or other antimicrobial substances may increase their effectiveness and reduce the risk of bacterial resistance [15; 25; 39]. It is also worth continuing to study the safety of eugenol and isoeugenol in antibacterial and antifungal therapy. Although these substances show promising properties, there is a need to evaluate the potential side effects and possible interactions with other drugs or substances.