## 1 Introduction  
Biochips, such as microfluidic, lab-on-a-chip, and organ-on-a-chip devices, and micro total analysis systems have opened a new door for medical and biological investigations due to capability of generating biologically relevant microenvironments and potential of single cell analysis [1; 2]. Generally, shape-controlled micropatterns grown on solid substrates by additive manufacturing are adequate for the generation of biomimetic cell-instructive environments, able to modulate specific cell responses for applications in tissue engineering, regenerative medicine, or cancer therapy. These surfaces and cell interfaces are needed for the next generation of biomaterials [3]. For example, in the cancer therapy,studies have shown that tumor cells may exhibit 'plasticity' in response to microenvironmental cues [4]. Interfacial geometry that dictates cancer cell tumorigenicity has revealed how the characteristics of micropatterns (e.g., size, shape, and perimeter characteristics) influence a population of tumor cells that can further guide cancer cells toward a stem cell-like state [5]. A clinical biochip consisting of more than 1000-nanoliter-sized microchambers was developed for single immune cell investigation [6]. The proteins secreted by individual cells were then captured by antibody stripes followed by multiplexed fluorescent detection. Such bioplatform proved successful ex vivo quantification of the polyfunctional diversity of tumor antigen-specific T lymphocytes. New biochip platforms consisting of microwell arrays were recently proposed for cell-based immunological studies with various applications related to immune cell secretion, cytotoxicity, migration, phenotyping, and immune cell engineering [7]. In addition, high-throughput cell trapping assays with single cell accuracy were efficiently developed into microfluidic lab-on-chip systems [8]. Meanwhile, lasers are increasingly getting important as tools for biomaterial modification or fabrication of biochips. The main advantages of laser techniques in comparison to other approaches are extensively addressed in the literature [9; 10]. One can mention the high processing versatility of a variety of materials together with fast, clean, flexible, and controllable microfabrication capability. During the last decade, laser technologies were explored in the field of biomimetics with new cutting-edge applications [11]. The state of the art of \"Laser Technology in Biomimetics\" covering a broad spectrum of materials, methodologies, and applications was comprehensively reported in [12]. This book covers aspects of laser-matter interaction of nanosecond pulsed lasers for 2D patterning using well-known deposition techniques, such as pulsed laser deposition (PLD) and matrix-assisted pulsed laser evaporation (MAPLE) for material coating and ultrashort laser pulses for 3D modification of materials. PLD and MAPLE employ pulsed laser beams, typically nanosecond laser pulses, to ablate solid targets in a vacuum. Then, the ablated material is deposited onto substrates opposed to the targets to form thin coatings. Different from PLD, which is used for inorganic coatings only, the MAPLE process employs milder conditions (laser energies one order of magnitude lower) adequate for coating any inorganic, organic, and hybrid materials with high versatility. Thus, the safe transfer and deposition of delicate compounds such as proteins and biopolymers are possible without affecting stability and deteriorating functionalities [11]. In addition, graphene oxide (GO) and GO nanocolloids (GON) can be deposited by MAPLE, which are very challenging matrices for drug release to target cancer cells. It has been very recently found that the MAPLE process provides safe concentration windows of GON for five different human melanoma cell lines as well as for nontransformed melanocytes and human dermal fibroblasts [13].