## 2 Results  
The chromatographic profile (chromatogram) of EOCw (Figure 1) revealed by gas chromatography with mass spectrometry (GC/MS), indicates a single substance with retention time = 31.299 min and maximum detection intensity (most concentrated). As can be seen in Table 1, the chemical composition of the EOCw presented as major constituents in ascending order of concentration: citronellol (16.91%), citronellal (17.31%), and geraniol (42.13%), followed by other compounds in lower concentrations. In Figure 2, it is graphically observed that the cytotoxic activity of the EOCw had a relatively high LC\({}_{50}\) of 96.56 \(\upmu\)g/mL, which is desired for products intended for human consumption. Table 2 shows, from a different perspective, the same promising result observed in Figure 2, which would be the low cytotoxicity exhibited by EOCw against mammalian cells that would normally be infected by the parasites; cell damage only showed to be significant from the concentration of 125 \(\upmu\)g/mL. According to Figures 3 and 4, the EOCw presented inhibition values considered very promising for the promastigote and epimastigote forms of _L. brasiliense_ and _T. cruzi_, with LC\({}_{50}\) values of 44.98 \(\upmu\)g/mL and 72.60 \(\upmu\)g/mL, for _Leishmana_ and _Trypanosoma_, respectively, with performance equivalent to that of Pentamidine, at a concentration of 125 \(\upmu\)g/mL. \begin{table}
\begin{tabular}{c c c} \hline \hline
**Components** & **RT (min) 1 ** & **(\%)** \\ \hline Limonene & 17.88 & 4.24 \\ Citronellal & 25.27 & 17.31 \\ Citronellol & 29.70 & 16.91 \\ Ceraniod & 31.29 & 42.13 \\ \(\beta\)-elemene & 39.37 & 2.69 \\ \(\delta\)-Cadinene & 46.65 & 1.05 \\ Elemol & 47.24 & 6.71 \\ Germacrene & 47.80 & 4.44 \\ Guaiol & 48.87 & 1.14 \\ Nerolidol & 52.70 & 3.38 \\ \hline Total & & 100.00 \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Chemical composition (%) of the essential oil of C. _winterianus_. Figure 1: GC/MS chromatogram of the essential oil of _C. winterianus_ with total ion current (TIC) peak reports. 1 Total ion Current. Figure 3: Cytotoxicity of the promastigotesote _L. brasiliensis_ treated with essential oil of _C. winterianus._ LC\({}_{50}\), with the confidence interval for oil at 95% (35.55–56.92). LC\({}_{50}\) was obtained through non-linear regression of means. \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Natural Product** & **Conc.**\(\mu\)**g/mL** & **\%C** & \(\pm\)**\%DS** \\ \hline _C. winterianus_ & 1000 & 0 & – \\  & 500 & 0 & – \\  & 250 & 2.15 & 0.49 \\  & 125 & 35.79 & 0.80 \\  & 62.5 & 89.96 & 0.70 \\  & 31.5 & 90.96 & 0.77 \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Survival of fibroblasts exposed to the essential oil of _C. winterianus._  
Figure 2: Cytotoxicity of the essential oil of _C. winterianus_, with the confidence interval for oil at 95% (87.07–107.10). LC\({}_{50}\) was obtained through non-linear regression of means. Additionally, observing Tables 3 and 4, it can be inferred that the EOCw showed survival percentage values considered very promising for the promastigote and epimastigote forms of _L. brasiliense_ and _T. cruzi_, respectively. Figure 5 shows the log-dose vs. cytotoxicity curves of EOCw for fibroblasts, promastigotes, and epimastigotes estimated through non-linear regression of means, demonstrating that the anti-kinetoplastid effect was more intense than the cytotoxic effect in fibroblasts. Figure 4: Cytotoxicity of the epimastigote _T. cruzi_ treated with essential oil of _C. winteranus_. LC\({}_{50}\) confidence interval for oil is 95% (50.63–104.10). LC\({}_{50}\) was obtained through non-linear regression of means.