## 2 Results
### RNA-Seq Analysis
\begin{table}
\begin{tabular}{c c c c c c c} \hline
**No** & **Name** & **CAS\#** & **RI** & **Exocarp** & **Pulp** \\ \hline  & & **No.** & & **HJU** & **R-HJH** & **HJU** & **R-HJH** \\ \hline \(1\) & \(\alpha\)-pinene & 80-56-8 & 7.103 & - & - & 5.58 \(\pm\) 0.29 & 2.53 \(\pm\) 0.18 \\ \(2\) & \(\beta\)-Myrcene & 123-35-3 & 8.613 & 813.58 \(\pm\) 74.28 & 268.86 \(\pm\) 42.92 & 169.82 \(\pm\) 7.53 & \\ \(3\) & \(\alpha\)-Phellandrene & 99-83-2 & 8.993 & 173.41 \(\pm\) 14.46 & 78.72 \(\pm\) 8.32 & 11.28 \(\pm\) 1.26 & 6.59 \(\pm\) 0.63 \\ \(4\) & D-Limonene & 599-27-5 & 9.757 & 8594.44 \(\pm\) 700.36 & 5721.00 \(\pm\) 827.98 & 774.94 \(\pm\) 43.00 & 398.28 \(\pm\) 22.36 \\ \(5\) & \(\gamma\)-Terpinene & 99-85-4 & 10.537 & 917.96 \(\pm\) 78.94 & 124.34 \(\pm\) 18.61 & 103.85 \(\pm\) 4.20 & 42.47 \(\pm\) 2.61 \\ \(6\) & Linalool & 78-70-6 & 11.679 & 1189.20 \(\pm\) 26.86 & 915.71 \(\pm\) 22.37 & 76.03 \(\pm\) 2.76 & 37.68 \(\pm\) 0.65 \\ \(7\) & Nonanal & 124-19-6 & 11.791 & 454.39 \(\pm\) 371.06 & 63.48 \(\pm\) 8.13 & 6.74 \(\pm\) 0.66 & 4.58 \(\pm\) 0.38 \\ \(8\) & \(\alpha\)-Terpineol & 10482-56-1 & 14.302 & 467.10 \(\pm\) 8.22 & 231.96 \(\pm\) 4.00 & 26.97 \(\pm\) 1.24 & 16.59 \(\pm\) 0.55 \\ \(9\) & Neral & 106-26-3 & 15.676 & 722.94 \(\pm\) 17.79 & 430.55 \(\pm\) 18.60 & 18.52 \(\pm\) 0.68 & 9.59 \(\pm\) 0.29 \\ \(10\) & Geraniol & 106-24-1 & 16.017 & 95.81 \(\pm\) 1.86 & 63.39 \(\pm\) 1.40 & 6.76 \(\pm\) 0.30 & 7.18 \(\pm\) 0.47 \\ \(11\) & Citral & 5392-40-5 & 16.485 & 1036.55 \(\pm\) 24.90 & 620.00 \(\pm\) 26.35 & 29.05 \(\pm\) 1.41 & 18.27 \(\pm\) 0.58 \\ \(12\) & Dodecanal & 112-54-9 & 20.102 & 91.55 \(\pm\) 7.81 & 88.59 \(\pm\) 17.96 & 4.73 \(\pm\) 0.48 & 1.72 \(\pm\) 0.03 \\ \(13\) & Caryophyllene & 87-44-5 & 20.532 & 324.47 \(\pm\) 33.88 & 134.35 \(\pm\) 36.49 & 12.14 \(\pm\) 1.31 & 2.16 \(\pm\) 0.21 \\ \(14\) & \(\gamma\)-Muurolene & 30021-74-0 & 21.925 & 223.37 \(\pm\) 25.60 & 127.79 \(\pm\) 33.25 & 9.73 \(\pm\) 1.07 & - \\ \(15\) & Germacrene D & 23986-74-5 & 22.070 & 1085.83 \(\pm\) 110.57 & 591.64 \(\pm\) 182.15 & 32.36 \(\pm\) 3.29 & 4.17 \(\pm\) 0.37 \\ \(16\) & \(\alpha\)-Muurolene & 10208-80-7 & 23.372 & 111.13 \(\pm\) 10.70 & 65.07 \(\pm\) 15.59 & 4.31 \(\pm\) 0.46 & - \\ \hline \end{tabular} \({}^{\ast}\)_\"_ indicated Chemical Abstracts Service. \"-\" indicated not detected.  
\end{table} 
Table 3: Main volatiles compounds (ug/mg) in two different fruits tissues of R-HJH and HJJH. KEGG pathway enrichment analysis revealed that the most enriched pathway was the metabolic pathway (821 DEGs), followed by the genetic information processing (129 DEGs), environmental information processing (120 DEGs) and organismal systems (120 DEGs) (Figure 3). Only 39 DEGs were assigned to cellular processes. When we focused on carotenoid biosynthesis, 12 unigenes were annotated according to KEGG analysis, with seven upregulated and five downregulated. Figure 3: Top 20 enriched KEGG pathways of differentially expressed genes (DEGs) in R-HJH compared with HJH. The X-axis shows the rich factor. A high q-value is represented by blue, and a low q-value is represented by red (\(p\) value \(<\) 0.05). The Y-axis represents the second KEGG pathway terms. The number of DEGs is represented by the size of the circle. Figure 2: The statistics of GO functional classification of differentially expressed genes (DEGs) in R-HJH compared with HJH. The X-axis indicates the number of DEGs. The Y-axis represents GO terms. All GO terms are grouped into three ontologies: blue represents biological processes, red represents molecular functions and green represents cellular components.