## 2 Materials and Methods
### Experimental Models  
(a) The 96-well plate model for Assessment of Minimum Antimicrobial Concentration. To assess the impact of OA and OCT on planktonic growth, 100 uL of Mueller-Hinton (BioCorp, Warsaw, Poland) broth (for _S. aureus_ and _P. aeruginosa_), TSB + 3% saccharose in case of _A. actinomycetemcomitans_ or RPMI with 2% glucose for _C. albicans_ was introduced into the wells of 96-well test plates (Gibco, Carlsbad, CA, USA). Afterwards, 100 uL of the OL or 100 uL of OCT, previously dissolved in 40 mL of saline (POCH, Gliwice, Poland), was introduced to the well. Next, geometric dilutions of individual antiseptic were obtained. Subsequently, 100 uL of microbial suspension (of density of 1 \(\times\) 10\({}^{5}\) CFU/mL established by densitometer Densitomat II, BioMerieux, Warsaw, Poland and subsequently by serial dilutions) was introduced into the wells of 96-well plates (Biofil, Warsaw, Poland). Then, the suspensions' absorbances were recorded at a 580 nm wavelength using a spectrometer ([PERSON], EnSpire Multimode Plate Reader, Waltham, MA, USA). Subsequently, the plates were subjected to incubation for 24 h at 37 \({}^{\circ}\)C in a shaker Lab Companion IST-3075R (Imgen Technologies, Alexandria, VA, USA) to provide optimal conditions for planktonic growth and to prevent microorganisms from forming a biofilm. Afterwards, the absorbance values were recorded once again. The sterile medium (containing no microbes) was a negative control for the experiment, while the medium containing microbes but no antimicrobial was utilized as a positive control for bacterial/fungal growth. If the spectrometric record showed the Minimum Antimicrobial value in a specific well of the plate, the whole content of that well was placed in an Eppendorf tube containing 1.8 mL of a neutralizer (polysorbate 80 (3.0%) + saponin (3.0%) + L-histidine (0.1%) + cysteine (0.1%)) for 5 min. Next, the whole suspension was placed in 8 mL of appropriate liquid media for the growth of the specific microorganisms applied in the test for 48 h. The lack of an increase in the turbidity observed after this time point was an additional indication of the lack of live microorganisms in the well. (b) The 96-well plate model for Assessment of Minimal Biofilm Eradication Concentration. The bacterial liquid cultures were diluted to the density of 0.5 McFarland and subsequently diluted to 10\({}^{5}\) cfu/mL in the same media as indicated in the assessment of the MBC value. Next, 100 uL of such diluted cultures were incubated in a 96-well plate at 37 \({}^{\circ}\)C/24 h. In that time, the biofilm formed. After incubation, the medium was removed. Next, geometric solutions of antiseptic (in sterile) media were introduced to the wells of the plate. The biofilms administered the antiseptic were incubated for another 24 h at 37 \({}^{\circ}\)C. Subsequently, the solutions were removed and sterile medium supplemented with tetrazolium chloride (TTC, Sigma-Aldrich, Darmstadt, Germany) at a 1% concentration was added. Next, the plates were incubated for 2 h at 37 \({}^{\circ}\)C. During these 2 h, live bacteria/fungi metabolized colorless TTC into red formazan. A lack of colorization in the first well next to a well that had turned red showed the MBEC value. These analyses were performed in three replicates. In case of _A. actinomycetemcomitans_, for which the formazan assay might be non-conclusive, an additional analysis was also performed, namely, 100 uL of neutralizing agent (polysorbate 80 (3.0%) + saponin (3.0%) + L-histidine (0.1%) + cysteine (0.1%)) was added to wells containing the aforementioned microbe's biofilm and antiseptic for 5 min. Subsequently, the whole content of the well was spotted on the BHI (Biomaxima, Lublin, Poland) agar and subjected to incubation for 24 h/37 \({}^{\circ}\)C. The lack of growth of _A. actinomycetemcomitans_ colonies in the spotted areas confirmed that the applied concentration of OCT or OA was the Minimal Biofilm Eradication Concentration.