## 3 Results
### Mycoremediation Assay  
Figure 1 shows the visual aspect of the four microcosms inoculated with the different SMSs after 40 days of incubation. The microcosms inoculated with SMS of _P. eryngi_ and _A. bisporus_ showed extensive colonization of the contaminated soil. The fungi _P. ostreatus_ and _L. edodes_ showed lower colonization than the previous ones. In the case of _P. ostreatus_ the colonization was not clearly observed until the 30 th day. Mean values of hydrolase and dehydrogenase activities were higher for the _A. bisporus_ microcosm than for the control. The other treatments showed higher enzymatic activities than the control but were not statistically different due to the high deviations between replicates (Table 2). Hydrolase and dehydrogenase activities were 17 and 4 times, respectively, higher for _A. bisporus_ than for the control, denoting the high metabolic activity in _A. bisporus_ treatment with respect to control. The mean values of ergosterol did not present significant differences between bioaugmented treatments (Table 2). However, the increment of ergosterol content in those treatments with respect to the control indicates the soil colonization of the four fungi from their respective SMS. The ligninolytic activity of the five microcosms was determined by three different activities: increase, versatile peroxidase and MnP. Lacase was proposed as a bioindicator of the mycelial growth of _A. bisporus_[41]. This enzyme only was detected in _A. bisporus_ and _P. eryngi_ treatments, denoting the extensive colonization of the soil and their metabolic activity. The lacase activity of _A. bisporus_ microcosms was significantly higher than the lacase activity of _P. eringii_ microcosms. Peroxidase activities were detected in control and all the bioaugmented microcosms irrespective of the SMS inoculated. However, no significant differences were detected for versatile peroxidase and MnP activities between treatments. The detection of peroxidase activity suggests the metabolic activity of the inoculated fungi and the inherent microbiota of soil. Figure 1: Visual state of the bioaugmented microcosms with four different SMS (_P. eryngi_, _L. edodes_, _P. ostreatus_ and _A. bisporus_) after 40 days of incubation. All the bioaugmented treatments reached a final concentration of TPHs significantly lower than the control. The _A. bisporus_ microcosms were the most effective treatment for the TPHs removal, reaching the lowest concentration of these contaminants at the end of the assay. This treatment showed a degradation rate of 48% higher than the control, followed by _L. edodes_ (34%), _P. ostreatus_ (29%) and _P. eryngii_ (12%). The assessment of the petroleum hydrocarbons removal was analyzed considering the aliphatic and aromatic nature of the contaminants. Moreover, the aliphatic and aromatic compounds were analyzed according to the length of their carbon chains (Table 3). Regarding the total aliphatic hydrocarbon removal, once again, all the bioaugmented microcosms showed significantly lower concentrations than the control microcosms at the end of the incubation. The SMS of _A. bisporus_ achieved the lowest concentration of total aliphatic hydrocarbons. In contrast, among the bioaugmented treatments, _P. eryngii_ was less effective for the removal of aliphatic hydrocarbons. The most abundant aliphatic hydrocarbons are compounds with chains of C\({}_{12}\)-C\({}_{16}\), C\({}_{16}\)-C\({}_{21}\) and C\({}_{21}\)-C\({}_{35}\). The concentration of lighter hydrocarbons (C\({}_{10}\)-C\({}_{12}\)) was one-fold