###### Abstract  
Repeated sprint ability (RSA) is defined as the ability to recover and maintain maximal effort during repeated sprints, recognised as a crucial performance component in team sports. The exercise mode used to test RSA may influence performance and the contributions of different energy systems. The primary aim of this study is to address the critical gap between traditional cycling-based anaerobic tests, such as the Wingate test, and the practical, sport-specific demands of running in field-based team sports. **Methods:** This study involved 32 professional soccer players (age: 21.2 \(\pm\) 1.3 years; height: 177.8 \(\pm\) 4.3 cm; and mass: 71.3 \(\pm\) 6.4 kg). They performed cycling- and running-based repeated sprint tests, with similar total sprint numbers, durations, and recovery times, on different days. Contributions from adenosine triphosphate-phosphocreatine (ATP-PCr), glycolytic, and oxidative systems were estimated through body weight, oxygen uptake (VO\({}_{2}\)), blood lactate (BLa), and the fast component of excess post-exercise oxygen consumption (EPOC). The VO\({}_{2}\) levels and heart rate (HR) were monitored during the rest (10 min), exercise, and recovery (15 min) phases in a breath-by-breath mode using a portable gas exchange system. BLa was measured before (at rest) and 1, 3, 5, 7, and 10 min after the running and cycling tests using a handheld portable analyser. A mono-exponential model estimated the ATP-PCr system contribution, calculated using the fast component of EPOC following the final sprint and the sum of the VO\({}_{2}\)-time integral during rest intervals. **Results** The cycling tests demonstrated significantly higher values for the peak power (PP), mean power (MP), and rate of perceived exertion (RPE) (\(p<0.05\)), while the heart rate peak and blood lactate responses were similar across all modalities. The fatigue index was notably higher in the running tests (\(p<0.05\)). Furthermore, the running tests showed greater contributions in both the percentage and absolute terms from the adenosine triphosphate-phosphocreatine (ATP-PCr) system (\(p<0.01\)), total energy demand (\(p<0.05\)), and total energy expenditure (TEE) (\(p<0.01\)). Notably, the running tests resulted in an increased phosphocreatine breakdown (\(p<0.05\)) and rapid phosphocreatine replenishment (\(p<0.01\)). A simple linear regression analysis highlighted a significant determination coefficient between these performance variables and the contributions of the energy systems, affirming the robustness of the results. The correlation heatmaps further illustrated these relationships, with higher correlations for the PP and MP across modalities (0.41), emphasising the moderate association between cycling and running tests in these metrics. **Conclusions:** This study elucidated the similarities and differences in energy system contributions and performance outcomes between a cycling and a running repeated sprint protocol, with a comparable total sprint time and work-rest ratio. The findings reveal that a running repeated sprint test elicits a higher energy demand and a higher contribution from the PCr energy system compared to cycling. Performance variables were not associated between running and cycling tests, suggesting those tests cannot be used interchangeably.  
energy demand; soccer; performance; Wingate; field test +
Footnote †: journal: Applied Sciences