# Nitrite: From Application to Detection and Development
## 8 Conclusions and Prospects
\begin{table}
\begin{tabular}{c c c} \hline
**Detection Methods** & **Advantages** & **Disadvantages** \\ \hline Ion Chromatography & High sensitivity and selectivity for accurate determination of nitrite in samples. Multiple anions can be determined simultaneously, making it suitable for the analysis of complex samples. High separation efficiency and good reproducibility, making it suitable for the analysis of large quantities of samples. & Instrumentation is expensive, with high operating and maintenance costs. Sample pre-treatment is complex and requires specialized operational skills. The analysis process is relatively slow and not suitable for rapid on-site testing. & Relatively low sensitivity and selectivity and susceptible to interference from other pigments in the sample. Requires the use of toxic color developers, with potential risks to the environment and operators. Analytical results may be affected by experimental conditions such as temperature, pH, etc. Low sensitivity and accuracy, not suitable for micro or trace analysis. & Relatively low sensitivity and selectivity and susceptible to interference from other pigments in the sample. Requires the use of toxic color developers, with potential risks to the environment and operators. Analytical results may be affected by experimental conditions such as temperature, pH, etc. Low sensitivity and accuracy, not suitable for micro or trace analysis. \\ Colorimetric & Simple and inexpensive to operate, suitable for initial screening and on-site testing. Qualitative results can be obtained quickly and are suitable for preliminary analysis of large samples. & Affected by sample color and turbidity and may require complex pre-treatment steps. Usually only suitable for specific types of samples and has limited applications. \\  & High sensitivity and low detection limit, suitable for the detection of micro and trace nitrite. & Equipment is costly and requires specialized operation and maintenance. Sensitive to environmental conditions, e.g., changes in temperature, and pH may affect the fluorescence signal. Requires the use of specific fluorescent probes, which may have stability and toxicity issues. \\ Biosensors & Rapid response allows for real-time monitoring. High sensitivity, can detect low concentrations of nitrite. Portable equipment, suitable for on-site testing and mobile laboratory use. & Sensor stability and repeatability may be challenged. Periodic calibration and sensor replacement may be required. For complex samples, additional sample pre-treatment steps may be required. \\  & High sensitivity and fast response time for rapid on-site detection. Specific electrode materials can be designed to improve selectivity and stability. & May be interfered with by other electroactive substances in the sample. The electrode surface may be contaminated and require periodic cleaning and maintenance. For some applications, complex electrode preparation and modification processes may be required. \\ \hline \end{tabular}
\end{table} 
Table 6: Analysis of the advantages and disadvantages of different detection methods. Traditional detection methods have long been used for their sensitivity and precision, while in recent years, research has led to innovations such as the preparation of more efficient detectors and the optimization of pre-treatment protocols. Among the various optical methods, fluorescence and colorimetric methods account for a large proportion, and both have high sensitivity and specificity. However, fluorescence methods are susceptible to interference from autofluorescence or background fluorescence, and colorimetric methods need to be further improved due to their low sensitivity and system instability. The vast majority of research nowadays has possessed new fluorescent probes for the purpose of achieving less matrix interference and accurate detection. In conclusion, nitrite has great challenges in future development.