## 3 Results
### Salicornia ramossissima Fatty Acids Profile  
In _S. ramossissima_ fourteen different fatty acids were identified. The most representative PUFA in the edible parts, considering cultured and wild haplotypes was the ALA fatty acid (Tables 3 and 4). In detail, cultured halophytes showed in edible part an ALA content of 7.02, 9.19, and 5.66 mg\({}^{-1}\) DW and 9.18, 7.96, and 5.41 mg mg\({}^{-1}\) DW for wild halophytes for spring, summer, and autumn, respectively. The roots of cultured and wild halophytes presented LA as the most abundant PUFA, with 5.24, 2.21, and 2.85 mg\({}^{-1}\) DW cultured halophytes and 5.28, 2.09, and 1.28 mg mg\({}^{-1}\) DW for wild halophytes during the spring, summer, and autumn, respectively. Oleic acid was the most representative MUFA with similar concentrations both in edible parts and roots in both cultured and wild halophytes. In both cultured and wild halophytes, palmitic acid was the most representative SFA, considering the edible parts and roots. Figure 3: Principal coordinates analysis of the fatty acid profile for cultured in aququonics (A) and wild (W) _H. portulacoides_ in spring, summer and autumn in edible parts and roots. \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline \multicolumn{6}{c}{**Wild**} \\ \cline{2-7}
**Fatty Acids** & \multicolumn{4}{c}{**Edible Plant Part**} & \multicolumn{4}{c}{**Root**} \\ \cline{2-7}  & \multicolumn{2}{c}{**Spring**} & **Summer** & **Autumn** & **Spring** & **Summer** & **Autumn** \\ \hline
**16:0** & 3.49 \(\pm\) 0.16 & 2.86 \(\pm\) 0.05 & 2.74 \(\pm\) 0.07 & 2.68 \(\pm\) 0.33 & 1.0  
Concerning to edible part, PERMANOVA test revealed significant differences among the FA profiles for cultured and wild specimens (Pseudo-F\({}_{1,16}\) = 222.46; \(p\) = 0.0001). This variability can be explained by the first two axes of the PCO performed, PCO1 axis explaining 75.2% of total variance plus PCO2 axis explaining 19.1% of total variation. During spring, summer, and autumn were also recorded significant differences in the FA profiles (Pseudo-F\({}_{2,16}\) = 606.97; \(p\) = 0.0001). For the root part of _S. ramossissima_, PERMANOVA test showed significant differences among the FA profiles for cultured and wild specimens (Pseudo-F\({}_{1,17}\) = 924.81; \(p\) = 0.0001), with the first two axis of the PCO analysis (Figure 4) explained 99.5% of total variance, with PCO1 axis explaining 90.4% of total variance and clearly separating cultured in aququonics from wild specimens. Displayed the same trend with significant differences among the spring, summer, and autumn (Pseudo-F\({}_{2,17}\) = 90.671; \(p\) = 0.0001). Wild and cultured _S. ramossissima_ presented a PUFA/SFA ratio > 0.45, indicative value of good nutritional quality see Tables 3 and 4.  
acid, with 5.49, 7.20, and 6.64 \(\upmu\)g mg\({}^{-1}\) DW. Regarding wild halophytes during the spring and summer, the most representative polyunsaturated fatty acids (PUFA) was ALA fatty acid displaying 6.17 and 6.19 \(\upmu\)mg\({}^{-1}\) DW, respectively. During the autumn, the most representative polyunsaturated fatty acid was linoleic acid (LA) fatty acid with 1.96 \(\upmu\)mg\({}^{-1}\) DW. Otherwise, the most abundant PUFA in roots was LA fatty acid, displaying 4.75, 2.81, and 3.03 \(\upmu\)mg\({}^{-1}\) DW for cultured halophytes and 1.97, 1.49, and 1.62 \(\upmu\)mg\({}^{-1}\) DW for wild halophytes during the spring, summer, and autumn, respectively. Oleic acid (18:1n-9) was the most representative monounsaturated fatty acid (MUFA) with similar concentrations both in edible parts and in the roots in cultured and wild halophytes during spring, summer, and autumn. Regarding saturated fatty acid (SFA), palmitic acid (16:0) was the most representative FA in both cultured and wild halophytes, considering the edible parts and roots during the spring, summer and autumn. The PERMANOVA test performed for edible part of _S. perennial_, showed significant differences among the FA profiles for cultured and wild specimens (Pseudo-F\({}_{1,17}\) = 47.39; \(p\) = 0.0001).