# GC- and UHPLC-MS Profiles as a Tool to Valorize the Red Alga Asparagopsis armata+
## 3 Results and Discussion
### GC-MS of Lipophilic Extract
\begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{2}{*}{**Rt (min)**} & \multirow{2}{*}{**Compound Identified \({}^{*}\)**} & \multicolumn{3}{c}{**Medium Content (SD)**} \\ \cline{3-5}  & & **mg/100 mg Extract** & **mg/100 g Dried Alga** & **mg/kg Fresh Alga** \\ \hline \multicolumn{5}{c}{_Fatty Acids_} \\ \hline
24.1 & Lauric acid (C12:0) \({}^{\text{b}}\) & 0.489 (0.024) & 6.11 (0.30) & 4.33 (0.21) \\ \hline
30.6 & Myristic acid (C14:0) \({}^{\text{b}}\) & 1.88 (0.07) & 23.5 (0.9) & 16.6 (0.6) \\ \hline
34.6 & Pentadecanoic acid (C15:0) \({}^{\text{b}}\) & 0.404 (0.012) & 5.05 (0.14) & 3.58 (0.10) \\ \hline
36.5 & Palmitoleic acid (C16:1) \({}^{\text{b}}\) & 0.767 (0.066) & 9.59 (0.82) & 6.80 (0.58) \\ \hline
36.8 & Palmitic acid (C16:0) \({}^{\text{a}}\) & 6.67 (0.29) & 83.4 (3.6) & 59.2 (2.6) \\ \hline
39.5 & Oleic acid (C18:1) \({}^{\text{a}}\) & 1.15 (0.13) & 14.4 (1.6) & 10.2 (1.1) \\ \hline
39.8 & Stearic acid (C18:0) \({}^{\text{a}}\) & 0.381 (0.017) & 4.76 (0.21) & 3.37 (0.15) \\ \hline \multicolumn{5}{c}{_Total_} & **11.7** & **147** & **104** \\ \hline \multicolumn{5}{c}{_Halogenated Compounds_} \\ \hline
4.2 & Chloro acetic acid \({}^{\text{b}}\) & 0.497 (0.049) & 6.21 (0.61) & 4.40 (0.43) \\ \hline
6.1 & Clorobutenol \({}^{\text{c}}\) & 0.502 (0.012) & 6.27 (0.15) & 4.45 (0.11) \\ \hline
8.5 & 3-Bromopropp-2-enoic acid \({}^{\text{c}}\) & 1.40 (0.20) & 17.5 (2.51) & 12.4 (1.78) \\ \hline
10.1 & Bromopyranivic acid \({}^{\text{c}}\) & 0.612 (0.061) & 7.65 (0.77) & 5.43 (0.54) \\ \hline
15.5 & 1,4-Dibromobuten-1-ol \({}^{\text{c}}\) & 12.4 (1.0) & 155 (12) & 110 (9) \\ \hline
19.1 & Bromotridecenol \({}^{\text{c}}\) & 0.842 (0.048) & 10.5 (0.6) & 7.47 (0.42) \\ \hline
19.3 & Isomer of bromotridecenol (19.1 min) \({}^{\text{c}}\) & 0.807 (0.061) & 10.1 (0.8) & 7.16 (0.54) \\ \hline
23.7 & Isomer of 1,4-dibromobuten-1-ol (15.5 min) \({}^{\text{c}}\) & 0.940 (0.019) & 11.7 (0.2) & 8.33 (0.17) \\ \hline \multicolumn{5}{c}{_Total_} & **18.0** & **224** & **159** \\ \hline \multicolumn{5}{c}{_Glycerol and derivatives_} \\ \hline
15.2 & Glycerol \({}^{\text{a,b}}\) & 0.151 (0.020) & 1.96 (0.20) & 1.39 (0.14) \\ \hline
40.4 & Glyceryl-glycoside \({}^{\text{b}}\) & 0.163 (0.031) & 2.04 (0.39) & 1.44 (0.27) \\ \hline
41.5 & 1-Monomyristin \({}^{\text{b,c}}\) & 0.356 (0.046) & 4.44 (0.57) & 3.15 (0.40) \\ \hline
43.3 & 1-Monopalmitin \({}^{\text{a}}\) & 0.353 (0.013) & 4.41 (0.16) & 3.13 (0.11) \\ \hline \multicolumn{5}{c}{_Total_} & **1.02** & **12.9** & **9.11** \\ \hline \multicolumn{5}{c}{_Sterols_} \\ \hline
48.4 & Cholesterol \({}^{\text{a}}\) & 0.617 (0.076) & 7.71 (0.95) & 5.47 (0.68) \\ \hline \multicolumn{5}{c}{_Aromatic compounds_} \\ \hline
16.1 & Phenyl acetic acid \({}^{\text{a}}\) & 0.683 (0.049) & 8.54 (0.61) & 6.06 (0.43) \\ \hline
18.6 & 4-Hydroxybenzaldehyde \({}^{\text{b}}\) & 0.158 (0.009) & 1.97 (0.11) & 1.40 (0.08) \\ \hline \multicolumn{5}{c}{_Total_} & **0.841** & **10.5** & **7.46** \\ \hline \multicolumn{5}{c}{_Other compounds_} \\ \hline
4.7 & Ethyl amine \({}^{\text{b}}\) & 0.058 (0.004) & 0.727 (0.054) & 0.516 (0.038) \\ \hline
8.1 & Glycolic acid \({}^{\text{b}}\) & 0.267 (0.037) & 3.33 (0.47) & 2.37 (0.33) \\ \hline
25.4 & Heptadecane \({}^{\text{b}}\) & 0.900 (0.043) & 11.2 (0.5) & 7.97 (0.38) \\ \hline \multicolumn{5}{c}{_Total_} & **1.23** & **15.3** & **10.9** \\ \hline \hline \end{tabular}  
\({}^{\text{Rt--Retention time. SD--Standard deviation. ${}^{*}$ All the compounds possessing hydroxyl groups are identified as the corresponding TMS derivatives. Compounds were identified by: ${}^{\text{a}}$ comparison with pure standards; ${}^{\text{b}}$ comparison with the GC-MS spectral libraries with similarity index higher than 94% and confirmation of the peaks at \(m\)/\(z\) values corresponding to the characteristic fragmentation pattern of the family in question; ${}^{\text{c}}$ interpretation of pattern fragmentation of MS spectrum according to published literature.  
\end{table}