## 3 Results and Discussion
### Main Results of Preclinical In Vivo Studies
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Experimental Groups**} & \multicolumn{5}{c}{**Control Time**} \\ \cline{2-6}  & **4 Hours** & **3 Days** & **7 Days** & **10 Days** & **14 Days** \\ \hline Control N 1 * & 44.0 (20.5–300.0) & 300.0 (300.0–300.0) & 300.0 (250.0–300.0) & 300.0 (300.0–300.0) & 300.0 (300.0–300.0) \\ \hline Control N 2 & 77.0 * (5.5–294.0) & 1.0 * (0.0–3.0) & 13 * (2.0–58.0) & 20 * (9.5–197.5) & 7.0 * (0.5–125.5) \\ \hline Main N 3 & 3.5 * (2.0–13.0) & 0.0 * (0.0–0.0) & 0.0 * (0.0–2.5) & 0.5 * (0.0–2.5) \\ \hline \hline \end{tabular}  
*–differences with control group N 1 are statistically significant at \(p<0.05\); #–differences with control group N 2 are statistically significant at \(p<0.05\). The statistical assessment method for the significance of differences in the results of the study was the Kruskal–Wallis test for independent samples.  
\end{table} 
Table 1: Influence of therapy regimens on the average number of colonies per 1 mL (Me (25–75%)). The main results of the histological studies of the experimental groups of animals are as follows. In control group \(N\)* 1, on the 14 th day of wound healing, granulation tissue was detected (Figure 8), represented by a large number of cells--mainly fibroblasts and fibroblasts, which is typical of phase II in the course of the wound process--proliferation. The granulation tissue contained a large number of plethoric vessels. The vessels were predominantly slit-like; their walls were not formed or only partially formed. Collagen fibers were arranged partially ordered. Inflammatory infiltration by lymphocytes, histiocytes, and neutrophils in the granulation tissue was pronounced in most of the animals. Figure 8: Granulation tissue of a rat wound in control group \(N\)* 1. Vascular plethora, pronounced inflammatory infiltration by lymphocytes, histiocytes, and neutrophils; cells dominate over the fibers (**A**,**B**). Massive overlays of exudate (**B**). Staining with hematoxylin and eosin. Mag. 200\(\times\). Figure 7: The condition of wound surfaces on the 14 th day after introducing the pathology. (**A**,**B**)—control group \(N\)* 1; (**A**)—wound under the scab; (**B**)—wound after removal of the scab (there is a pronounced wound surface with purulent exudate); (**C**)—control group \(N\)* 2 (levomekol), there is a decrease in the wound surface, the wound contains exudate; (**D**)—the main group (levomekol + irradiation with the “Zarnitsa-A” apparatus), there is a pronounced decrease in the size of the wound defect and an absence of exudate. In control group \(N\) 2, compared with control group \(N\) 1, the granulation tissue in most of the animals contained few vessels (Figure 9). The vessels were predominantly slit-shaped, but some of them were rounded. In the slit-shaped vessels of most of the animals, the walls were partially formed, and collagen fibers were partially ordered. Inflammatory infiltration in the granulation tissue of most of the rats was mild. In general, the histological picture of the wound defects in animals in control groups \(N\) 1 and \(N\) 2 corresponds to the second phase of the course of the wound process, i.e., proliferation. In the main group, blood vessels were predominantly round with formed walls in the granulation tissue (Figure 10). Collagen fibers were arranged in an orderly manner. Histologically mature scar tissue was represented by parallel, dense bundles of collagen containing few blood vessels and cells compared with intact tissues. Inflammatory infiltration by lymphocytes, neutrophils, and histiotes in the granulation tissue of all the animals was weakly expressed. Thus, in contrast to the control groups, given the morphological characteristics, phase III of the wound process in the main group was observed, showing that accelerated wound healing took place. Figure 10: Granulation tissue of a rat wound in the main group (levomekol with irradiation).