## 1 Introduction  
Grapevine is capable of synthetizing a wide variety of phenolic compounds, such as phenolic acids (hydroxybenzoic and hydroxycinnamic acids), stilbenes and flavonoids, with important protecting functions against biotic and abiotic stresses. Flavonoids act among others as antioxidants and UV-protectants and have important health protecting effects by consumption of berries, wine or leaf extracts [1; 2; 3; 4; 5; 6]. The flavonoid composition tends to be specific, mainly determined by genetic factors, but their amount is highly dependent on viticultural and environmental factors such as light, temperature, and water status; furthermore on insect and pathogen attacks or phenology [7; 8; 9; 10]. acclimation to sunlight includes a variety of metabolic responses [11]. Among these, flavonoids provide multiple roles in photoprotection as potential UV absorbers and as antioxidants [12; 13; 14; 15]. According to their micro-localization in leaf tissues, flavonoids are expected to aid both physical (screening) or chemical (antioxidant) defense functions. The possibility of direct solar UV-screening by flavonoids is supported by their presence in epidermal cell vacuoles and trichomes [16; 17; 18]. On the other hand, the importance of UV-shielding has been challenged by a generally better absorption of lower energy UV-A, than of potentially more damaging UV-B by flavonoids [19], in favor of potential antioxidant functions [20]. The positive relationship between sunlight exposure and increased flavonoid accumulation have been noticed by several researchers studying berry flavonoids and their biosynthetic pathways [21; 22; 23], while grapevine leaf flavonoids as important participants in acclimation processes of the plant are less studied.  
The main objective of our work was to analyze and localize the leaf polyphenics of grapevine varieties in terms of effect of natural sunshade conditions in the canopy, using a high performance liquid chromatography and accurate mass, high-resolution mass spectrometry as well as laser scanning microscopy. Grapevine (_Vitis vinifera_ L.) leaves are rich in polyphenols and thus provide an excellent specimen for these studies. Traditionally, the growing method (see in Section 3.1) consistently provides a relatively stable shaded condition for leaves developing inside the canopy as opposed to fully sun exposed ones. Goher white, Goher altering and Goher red, belonging to the Goher concluta, show very close genetic relationship [24], but differ in the regulation of the anthocyanin biosynthesis in both their leaves and berries. However, the nonanthocyanin polyphenic composition of the leaves of these varieties is unstudied. Since genetic differences among phenotypes may render the importance of environmental factors on phenolics production moot [25], these varieties offer an amenable model to determine the importance of genetic and environmental factors influencing grapevine phenolic composition. Nevertheless, the native varieties bring diversity; therefore, their characterization is critical. Good adaptation to local environmental conditions could contribute to unique composition, which finally reflects in sensory properties of grape and in prepared wines. The results of this study give information on the accumulation and localization of nonanthocyanin phenolic compounds in the leaves of three grapevine varieties native to Hungary.