## 3 Results
### Effects of TJ on Cell Viability in 3T3-L1 Cells
The data are shown as mean \(\pm\) standard deviation (SD; n = 3), and statistical analyses were conducted by [PERSON]’s multiple range tests after one-way ANOVA. Each lowercase alphabet indicates within-figure differing significance (\(p<0.05\)). Figure 2: The effects of the TJ in the cAMP level (**A**), intracellular FA (**B**), TG (**C**), and glycerol release (**D**) related to the adipogenesis mechanism in differentiated 3T3-L1 cells. NC, normal control; DC, differentiated control; PC1, catechin 100 \(\upmu\)g/mL; PC2, catechin 200 \(\upmu\)g/mL; JT (10, 50, 100, or 200 \(\upmu\)g/mL). The data are shown as mean \(\pm\) standard deviation (SD; n = 3), and statistical analyses were conducted by [PERSON]’s multiple range tests after one-way ANOVA. Each lowercase alphabet indicates within-figure differing significance (\(p<0.05\)). The TJ Regulated the Protein Expression Levels Related to the Adipogenesis Mechanism in Differentiated 3T3-L1 Cells  
Upon measuring the protein expression level related to the mechanism of suppressing adipogenesis, the phospho-MAPK/MAPK ratio was significantly reduced in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (1.49 \(\pm\) 0.23, 1.30 \(\pm\) 0.06, 0.78 \(\pm\) 0.06, and 0.40 \(\pm\) 0.03, respectively) in a dose-dependent manner relative to that of the DC group (1.90 \(\pm\) 0.03) (Figure 4A,B). The phospho-CREB/CREB ratio was significantly reduced in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (1.40 \(\pm\) 0.03, 0.94 \(\pm\) 0.06, 0.97 \(\pm\) 0.11, and 0.75 \(\pm\) 0.12, respectively) in a dose-dependent manner relative to that of the DC group (1.28 \(\pm\) 0.03) (Figure 4A,C). The C/EBP\(\beta\)/actin ratio was significantly reduced in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (1.02 \(\pm\) 0.06, 0.87 \(\pm\) 0.06, 0.29 \(\pm\) 0.03 and 0.21 \(\pm\) 0.03, respectively) in a dose-dependent manner relative to that of the DC group (0.96 \(\pm\) 0.15) (Figure 4A,D). The PPAR\(\gamma\)/actin ratio was significantly reduced in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (1.43 \(\pm\) 0.05, 1.17 \(\pm\) 0.12, 0.81 \(\pm\) 0.15, and 0.72 \(\pm\) 0.08, respectively) in a dose-dependent manner relative to that of the DC group (1.48 \(\pm\) 0.05) (Figure 4A,E). The C/EBP\(\alpha\)/actin ratio was significantly reduced in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (0.97 \(\pm\) 0.04, 0.82 \(\pm\) 0.03, 0.71 \(\pm\) 0.02, and 0.59 \(\pm\) 0.06, respectively) in a dose-dependent manner relative to that of the DC group (1.23 \(\pm\) 0.05) (Figure 4A,F). The FABP\(\gamma\)/actin ratio was significantly reduced in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (1.21 \(\pm\) 0.05, 1.20 \(\pm\) 0.15, 0.69 \(\pm\) 0.05, and 0.54 \(\pm\) 0.05, respectively) in a dose-dependent manner relative to that of the DC group (1.21 \(\pm\) 0.15) (Figure 4A,G). The leptin/actin ratio was significantly reduced in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (1.33 \(\pm\) 0.05, 1.09 \(\pm\) 0.10, 0.87 \(\pm\) 0.05, and 0.54 \(\pm\) 0.03, respectively) in a dose-dependent manner relative to that of the DC group (1.46 \(\pm\) 0.05) (Figure 4A,H). The G6P/actin ratio was significantly reduced in the TJ 10, 50, 100, and 200 \(\mathrm{\SIUnitSymbolMicro g}\)/mL groups (1.30 \(\pm\) 0.05, 1.15 \(\pm\) 0.06, 0.72 \(\pm\) 0.08, and 0.61 \(\pm\) 0.08, respectively) in a dose-dependent manner relative to that of the DC group (1.40 \(\pm\) 0.10) (Figure 4A,J). Figure 4: Effects of the TJ on adipogenesis-related proteins in 3T3-L1 cells: **(A)** Western blot showing the expression levels of the proteins, **(B)** p-MAPK/MAPK, **(C)** p-CREB/CREB, **(D)** CEBP\(\beta\)/actin, **(E)** PPAR\(\gamma\)/actin, **(F)** CEBP\(\alpha\)/actin, **(G)** FABP\(\gamma\)/actin, **(H)** leptin/actin, **(I)** G6P/actin, and **(J)** PCK/actin.