## 4 Conclusions  
The NMR-based characterization allowed to obtain the metabolite profiling of hybrid tomato cultivars essential to define nutritional properties. This untargeted approach allowed to characterize each cultivar: King Creole showed the highest content of malic acid, whereas Bamano and Sugarland were characterized by high levels of glucose and fructose. Fiaschetta native cultivar showed the highest level of lactic acid and MUFA and the lowest levels of sugars, whereas DulceMiel cultivar was characterized by high levels of both sugars and acids. The content of water-soluble metabolites in analyzed tomatoes is generally consistent with the literature data obtained by NMR-based characterization of other tomato cultivars (\"Ailsa Craig\" [17], \"Palmiro\" and \"Clotilde\" [10], \"Moneymaker\" [12]). Nevertheless, a few metabolites showed either relatively lower (malic acid) or higher content (alanine, threonine) with respect to the literature data mentioned above.  
The knowledge of the nutritional profile and chemical composition of hybrid tomatoes may represent an important tool for fresh market, industries and customers, driving them to a more targeted and optimized use of the fruits.  
The following are available online at [[http://www.mdpi.com/2076-3417/10/5/1887/s1](http://www.mdpi.com/2076-3417/10/5/1887/s1)]([http://www.mdpi.com/2076-3417/10/5/1887/s1](http://www.mdpi.com/2076-3417/10/5/1887/s1)), Figure S1: \({}^{1}\)H-\({}^{1}\)H TOCSY spectrum of hydroalcoholic extract from \"Fiaschetta di Fondi\" tomato fruits in 400 mM phosphate buffer/D\({}_{2}\)O 1 mM TSP, Figure S2: \({}^{1}\)H-\({}^{13}\)C HSQC spectrum of hydroalcoholic extract from \"Fiaschetta di Fondi\" tomato fruits in 400 mM phosphate buffer/D\({}_{2}\)O 1 mM TSP, Figure S3: \({}^{1}\)H-\({}^{13}\)C HMBC spectrum of hydroalcoholic extract from \"Fiaschetta di Fondi\" tomato fruits in 400 mM phosphate buffer/D\({}_{2}\)O 1 mM TSP, Figure S4: \({}^{1}\)H-\({}^{1}\)H TOCSY spectrum of organic extract from \"Fiaschetta di Fondi\" tomato fruits in CDCl\({}_{3}\)/CD\({}_{3}\)OD 2:1 v/v mixture, Figure S5: \({}^{1}\)H-\({}^{13}\)C HSQC spectrum of organic extract from \"Fiaschetta di Fondi\" tomato fruits in CDCl\({}_{3}\)/CD\({}_{3}\)OD 2:1 v/v mixture, Figure S6: \({}^{1}\)H-\({}^{13}\)C HMBC spectrum of organic extract from \"Fiaschetta di Fondi\" tomato fruits in CDCl\({}_{3}\)/CD\({}_{3}\)OD 2:1 v/v mixture.  
Conceptualization, A.P.S. and C.I.; methodology, A.P.S.; validation, A.P.S. and M.S.; formal analysis, C.I., S.C. and M.S.; investigation, C.I. and M.S.; resources, A.M.G.; data curation, A.M.G.; writing--original draft preparation, C.I.; writing--review and editing, A.P.S.; visualization, C.I., L.M. and A.P.S.; supervision, L.M. and A.P.S.; project administration, L.M.; funding acquisition, L.M. All authors have read and agreed to the published version of the manuscript.  
This work has been supported by REGIONE LAZIO, \"e-ALIERB\" Project LR13/2008-Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Universita di Roma and by Italian Ministry of Education, Universities and Research-Dipartimenti di Eccellenza-L. 232/2016  
The authors acknowledge Terra del Sole farm for the samples collection.  
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.  
Figure 5: Dendrogram (TCA) showing the clustering, based on NMR data (both hydroalcoholic and organic metabolites quantified) of F: Fiaschetta, B: Bamano, DM: DulceMiel, S: Sugarland, KC: King Creole. Two levels corresponding to a different number of groups are marked.