## 2 Materials and Methods
### Analysis of Fatty Acid Methyl Esters  
For fatty acid determination, total fat was extracted according to the method described by [PERSON] and [PERSON] [PERSON] and [PERSON] (1996), with modifications. In brief, 10 g of sample was homogenized with NaCl (1%), in order to achieve a moisture of 80%, 8 mL of chloroform and 20 mL of methanol for 30 s (at 12,000 rpm) (AllegraTM X-22 Centrifuge, Beckman Coulter, Brea, CA, USA). Then, 10 mL of chloroform was added to the mixture and homogenized, and after that, 10 mL of NaCl (1% in distilled water) was put in and mixed. Afterwards, the organic phase (lower layer containing chloroform and fat) was separated from the aqueous layer (upper phase) and residues (\"meat phase\") by centrifugation at 4000 rpm for 10 min. Finally, the lower phase was transferred to a test tube (previously weighed) and evaporated to dryness using N\({}_{2}\) on a TurboVap(r) LV evaporator (1.2 bar nitrogen pressure with water bath temperature at 56 \({}^{\circ}\)C) (Zymark Corporation, Hopkinton, MA, USA). After cooling at room temperature, the test tube was weighed and the amount of fat was calculated by difference and reserved for transesterification. Fatty acid transesterification was carried out in accordance with the procedure formerly described by [PERSON] et al. [PERSON] et al. (2016), with some modifications: 20 \(\pm\) 5 mg of extracted fat was dissolved in 1 mL of toluene and successively mixed with 2 mL of sodium methoxide (0.5 N) solution, the mixture was allowed to stand for 15 min at room temperature after being vortexed for 10 s. Then, 4 mL of a H\({}_{2}\)SO\({}_{4}\) solution (10% of H\({}_{2}\)SO\({}_{4}\) in methanol) was added, vortexed for 10 s and allowed to stand for 5 min. At this point, 2 mL of saturated sodium bicarbonate solution was added and vortexed again. For the extraction of fatty acid methyl esters, 1 mL of hexane was added to the samples, vortexed for 10 s, and 970 \(\upmu\)L of organic phase was then transferred to an appropriate vial for gas chromatography (GC) and mixed with 30 \(\upmu\)L of C19:0 (10 mg/mL; nonadecanoic acid; internal standard). Separation and quantification of fatty acids methyl esters (FAMEs) were performed using a gas chromatograph (GC-Agilent 7890B, Agilent Technologies, Santa Clara, CA, USA) equipped with a flame ionization detector (FID) and a PAL RTC-120 autosampler with a liquid injection tool (Pal System). One microliter of sample was injected in split mode (1:50). The injector was maintained at 250 \({}^{\circ}\)C and 64.2 mL/min of total flow. A DB-23 fused silica capillary column (60 m, 0.25 mm i.d., 0.25 \(\upmu\)m film thickness; Agilent Technologies) was employed for FAMEs separation. Chromatographic conditions were as follows: initial oven temperature of 50 \({}^{\circ}\)C (held for 1 min), first ramp at 25 \({}^{\circ}\)C/min to 175 \({}^{\circ}\)C, second ramp at 3 \({}^{\circ}\)C/min to 230 \({}^{\circ}\)C (held for 3 min), and third ramp at 2 \({}^{\circ}\)C/min to a final temperature of 235 \({}^{\circ}\)C (held for 3 min). Helium was used as a carrier gas at a constant flow-rate of 1.2 mL/min, with the column head pressure set at 22.9 psi. The FID detector was maintained at 280 \({}^{\circ}\)C, while the operational flows were set as 40 mL/min of H\({}_{2}\), 450 mL/min of air and 30 mL/min of makeup flow (He). The detector signal was recorded at 10 Hz data rate. The total time for chromatographic analysis was 32.83 min. Data acquisition and equipment control was carried out using the software Mass Hunter GC/MS Acquisition B.07.05.2479 (Agilent Technologies), while the data analysis was carried out with the software Mass Hunter Quantitative Analysis B.07.01.