# Genomic Insights into Two Endophytic Strains:
## 4 Discussion  
* Authentication of the endophytic bacteria strains as plant growth enhancers. The quest for cheaper, readily available, and natural resource-based alternatives to synthetic agrochemicals as a means of achieving highly intensified improvements in sustainable agricultural productivity is a must in the midst of, among other factors, declining agricultural input resources, climatic change variations, famine, urbanization, war, and the increasing number of people in the world to feed. Endophytic microbiota re-search is being looked into as a veritable means of achieving the objective of boosting food safety and security in a sustainable manner [37]. In this study, the endophytic bac  
Figure 5: (**A**) Endophytic Stenotrophomonas geniculata strain NWUBe21 subsystem overview showing different functional/metabolic roles. (**B**) Endophytic Pseudomonas carmis strain NWUBe30 subsystem overview showing different functional/metabolic roles. terial strains _Stenotrophomonas geniculata_ NWUBe21 and _Pseudomonas carnis_ NWUBe30 that were isolated from the tissues of _Vigna unguiculata_ were authenticated as possessing plant growth-improving characteristic features. The results obtained from both of the endophytic strains indicated their potency to solubilize phosphate, produce siderophore, secrete plant hormones, and produce auxin and ammonia, as well as other plant growth-enhancing metabolites. Similar results of endophytic bacterial strains that possess plant growth-stimulating features have been reported [38; 39; 40]. In order to further examine the genetic basis of the plant growth-promoting traits of _Stenotrophomonas geniculata_ strain NWUBe21 and _Pseudomonas carnis_ strain NWUBe30, the genomic exploration of the two endophytic bacteria was conducted in order to determine the genes responsible for the important secondary metabolites secreted, as well as the genes responsible for the plant growth-stimulating features. The highlights of the beneficial features in the genome of the endophytic bacterial strains that confer plant growth-promoting attributes are summarized below. * Genetic components that are involved in plant growth enhancement. The genome exploration of the two endophytic bacterial strains _S. geniculata_ NWUBe21 and _P. carnis_ NWUBe30 indicated the presence of various genes that play vital roles in enhancing plant growth traits. * Iron acquisition and metabolism. The genomes of the two endophytic bacterial strains contain genes that are responsible for siderophore production and iron metabolism. These include the iron siderophore receptor protein, the iron siderophore sensor protein, the ferrous iron transport periplasmic protein EfeO, the ferrous iron transport permease EfeU, and the ferrous iron transport peroxidase EfeB. Iron is of vital importance for plant growth and survival. The presence of various iron-acquisition and siderophore genes in the genome of these two bacterial strains is responsible for the development of plants by making iron readily available for utilization by the plants [41; 42]. * Motility and Chemotaxis  
The movement and attachment of micro-organisms to plants are crucial for effective colonization [43]. Different genes that aid the attachment and movement of these two microbes in plants are present in their genomes. They include the chemotaxis protein CheV, the flagellar L-ring protein FlgH, the flagellar biosynthesis protein FlhA, the predicted signal transduction protein, and the flagellar motor rotation protein MotA. * Nitrogen Metabolism  
Nitrogen remains a key macronutrient that is essential for plant growth; hence, it is regarded as a key nutrient that is generally deployed for agricultural production [44]. Various nitrogen metabolism genes are present in the genome of the two endophytic bacteria, such as NorD--the nitric oxide reductase gene, the ammonium transporter, nitrilase, NorR, and the glutamate ammonia ligase adenylyltransferase gene. * Phosphorus Metabolism. Phosphorus is another key mineral that is essential for plant growth and vitality.