## 5 Conclusions  
To our knowledge, the present study represents the first assessment of \(Nm\)E influence on the cancer cell (HepG2) cycle profile analysis, and ROS production, of the antimicrobial activity of both \(Cs\)E and \(Nm\)E. The data generated during the current study emphasize our hypothesis, namely, that \(Nm\)E has anti-cancer properties that might be promising, and can be developed in the future as an anti-cancer drug for cancer therapy outcomes, and also that \(Cs\)E acts as a vital source for antimicrobial drug discovery. Therefore, the findings in this study recommend increasing research in this field, which can have solutions in treating many intractable diseases. **Author Contributions:** [PERSON], [PERSON], [PERSON], [PERSON], [PERSON], [PERSON] and [PERSON] performed the experiments. [PERSON] and [PERSON] collected sponge specimens from Red Sea via SCUBA diving. [PERSON] and [PERSON] conceived, designed, and supervised the study, and provided key insights into the planning of the project, interpretation of the data, revised the final manuscript for intellectual content and coordinated the study, as well as all other authors. [PERSON], [PERSON], [PERSON], and [PERSON] analyzed the data and wrote the first draft of the manuscript. [PERSON]-O. and [PERSON]R. Contributed reagents, materials, laboratory, and grant support for the project. [PERSON] and design the study, validation, supervision and revised the final manuscript for intellectual content and coordinated the study. All authors have read and agreed to the published version of the manuscript. **Funding:** This research project was supported by a grant from the Researchers Supporting Project number (RSP2021/114), King Saud University, Riyadh, Saudi Arabia. **Institutional Review Board Statement:** Not applicable. **Informed Consent Statement:** Not applicable. Data Availability Statement: The datasets generated during and/or analyzed through the current study are available from all authors. The authors would like to extend their sincere appreciation to the Research Supporting Project number: RSP-2021/114, King Saud University, Riyadh, Saudi Arabia. The authors declare no conflict of interest. References  
[PERSON]; [PERSON]. Novel extracts from _Callyspongia siphonella_ and _Negombata magnifica_ sponges from the Red Sea, induced antiproliferative and proapoptotic activity in HepG-2, MCF-7, and Caco-2 cancer cell lines. _Egypt. J. Aqua. Biol. Fish._**2020**, _24_, 319-347. [CrossRef]  
[PERSON]; [PERSON] [PERSON]; [PERSON]; [PERSON]; [PERSON]; [PERSON] [PERSON] GC-MS analysis of bioactive components in six different crude extracts from the Soft Coral (_Sinularia maxim_) collected from Ras Mohamed, Aqaba Gulf, Red Sea, Egypt. _Egypt. J. Aquat. Biol. Fish._**2020**, _24_, 425-434. [CrossRef]  
[PERSON]; [PERSON]; [PERSON]; [PERSON] [PERSON]; [PERSON]; [PERSON]. Antiproliferative Activity, Proapoptotic Effect, and Cell Cycle Arrest in Human Cancer Cells of Some Marine Natural Product Extract. _Oxidative Med. Cell. Longex._**2020**, _2020_, 7948705. [CrossRef] [PubMed]  
[PERSON]; [PERSON] [PERSON]; [PERSON]; [PERSON]; [PERSON] Eco friendly Synthesis of Zinc Oxide Nanoparticles by Marine Sponge. _Spongia officinalis_: Antimicrobial and Insecticidal Activities against the Mosquito Vectors, _Culex pipiens_ and _Anopheles pharemisis. BioNanoScience_**2021**. [CrossRef]  
[PERSON]; [PERSON] [PERSON]; [PERSON] [PERSON]; [PERSON]; [PERSON]. Effect of human activities on biodiversity in Nabq Protected Area, South Sinai, Egypt. _Egypt. [PERSON]. Res._**2019**, _45_, 33-43. [CrossRef]  
[PERSON]; [PERSON] [PERSON]; [PERSON] [PERSON]; [PERSON]; [PERSON] [PERSON]; [PERSON]; [PERSON] Effects of recreational diving and snorkeling on the distribution and abundance of surgeonfishes in the Egyptian Red Sea northern islands. _Egypt. [PERSON]. Res._**2020**, _46_, 251-257. [CrossRef]  
[PERSON]; [PERSON]; [PERSON]; [PERSON] [PERSON]; [PERSON] Impact of tourism and fishing on the coral reef health along the west coast of the Gulf of Aqaba, Red Sea, Egypt. _Egypt.