## 3 Results and Discussion
### Bioactive Compounds in Raspberries Fruits
In the present study, the content of total polyphenols in wild raspberry is the highest, as mentioned earlier; this may be due to the highest dry matter content in these plants. Probably, the raspberries studied differed in climatic and agrotechnical conditions during growth, which could have a significant impact on the amount of analyzed compounds. Contaminants from fertilizers used in conventional agriculture and the lower nutritional quality of the fruit could affect the low concentration of these compounds. According to [PERSON], et al. [40], the anthocyanin content in 100 g of dry raspberry fruit _Polana_ variety was 196.23 mg 100 g\({}^{-1}\) of cyanidin-3-glucose. Similar results were obtained by other authors [42; 44]. These results corresponded to conventionally grown raspberries from the 2020 crop. Other authors have obtained higher results for raspberry anthocyanin content. [PERSON], et al. [39] examining the species of the same raspberry variety obtained the results of 513.55 mg 100 g\({}^{-1}\) DW, which suggests that raspberries grown in Serbia may have a higher content of anthocyanins. The deep red color of the raspberry proper is related to its composition, including its anthocyanin content, the concentration of which is influenced by several factors, such as the variety, stage of ripening and climatic and soil characteristics of the growing sites, among others. Anthocyanins, and by extension raspberry color, are important to raspberry growers because they improve consumers' perception of quality. Sun exposure increases the concentration of these water-soluble pigments because it promotes the expression of flavonoid biosynthesis genes in the skin of these fruits. On the other hand, excessively high temperatures lead to inhibition of their biosynthesis [26]. In the results of this work, the highest anthocyanin content was obtained in organic raspberries. The reason for this may be that in organic production, plants activate their natural defense system against diseases and pests, so they synthesize more polyphenic compounds, which have defensive functions in plants. Better health-promoting properties are also due to higher nutritional value and lower levels of contaminants, which are residues of agricultural chemicals from conventional farms [45]. The reason for the lower anthocyanin content of wild raspberry may have been, among other things, too little light [46]. Raspberry fruits tested by other researchers had lower antioxidant activity as determined by ferric-reducing antioxidant power [42]. [PERSON], et al. [40] showed that the _Polana_ cv. of raspberry fruit had an antioxidant activity at the level of 156.60 mg TE g\({}^{-1}\) DW (ABTS assay) and 150.31 mg TE g\({}^{-1}\) DW (FRAP method). Our results obtained by the FRAP method are higher than those presented by the authors, while the results of antioxidant activity obtained by the ABTS assay were similar to the results of this authors. Total carotenoids found in raspberries according to [PERSON], et al. [47] are 0.47 mg g\({}^{-1}\) DW. Polish nutritional tables [38] present these data at 0.02 mg/100 g (retienol + \(\beta\)-carotene). Other studies have found that the \(\alpha\)-carotene content of raspberries ranged from 20 to 80, and beta caroten from 40 to 160 \(\mu\)g g\({}^{-1}\) DW; the seeds had a low caroten content, but were rich in zeaxanthin and lutein [48]. The carotenoid profile of raspberries is highly variable and depends on the species origin, fruit phenotype, growing site, sunlight and the period in which the fruit was harvested.