## 2 Materials and Methods
### Protection Treatments  
The research factor was protection against infectious diseases, which included horseradish seedling treatment before planting and foliar application of preparations.Due to the high risk of insect infestation, it was also necessary to activate pest control. During the growing season, the horseradish was protected according to the scheme presented in Table 1. The control of weeds throughout the entire experiment was conducted mechanically. The following agents were used in the experiment: chemical, biological, as well as biostimulants included in the list below. \begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{3}{*}{**Treatments**} & \multirow{3}{*}{**Root Cuttings**} & \multicolumn{3}{c}{**TheFoliar Application**} \\ \cline{3-5}  & & **Fungicide** & **Insecticide** & **Biostimulants** \\  & **Before Planting** & **Preparations** & **Preparations** & **Preparations** & **Biostimulants** \\ \hline \multirow{6}{*}{Control} & \multirow{6}{*}{Untreated} & \multirow{6}{*}{Without protection} & \(1\times\) Decis Mega 50 EW, 0.151-ha\({}^{-1}\) & \\  & & & \(1\times\) Proteus 110 OD, 0.61-ha\({}^{-1}\) & \\  & & & \(1\times\) Sumi Alpha 050 EC, 0.21-ha\({}^{-1}\) & \\  & & & \(4\times\) Dipel\({}^{\text{\text{\textregistered}}}\) & \\  & & & \(WG\),1 kg-ha\({}^{-1}\) & \\ \hline \multicolumn{5}{c}{Chemical protection} \\ \hline \multirow{6}{*}{ChP} & \multirow{6}{*}{Topsin M 500 SC} & \multirow{6}{*}{2 \(\times\) Dichane Neo Tec} & \(2\times\) Decis Mega 50 EW, 0.151-ha\({}^{-1}\) & \\  & & & \(2\times\) Dichane Neo Tec & \(2\times\) Proteus 110 OD, 0.61-ha\({}^{-1}\) & \\  & & & \(2\times\) Amistar Opti & \(1\times\) Nurelle D 550, 0.61-ha\({}^{-1}\) & \\  & & & \(480\) SC, 2.51-ha\({}^{-1}\) & \(1\times\) Nurelle D 550, 0.61-ha\({}^{-1}\) & Not used \\  & & & \(2\times\) Ridomil Gold MZ & \(0.6\)1-ha\({}^{-1}\) & \\  & & & \(2\times\) Ridomil Gold MZ & \(1\times\) Sumi Alpha 050 EC, 0.21-ha\({}^{-1}\) & \\  & & & \(2\times\) kg-ha\({}^{-1}\) & \(1\times\) Bulldock 025 & \\  & & & & EC,0.31-ha\({}^{-1}\) & \\ \cline{3-5}  & & & \multicolumn{3}{c}{First treatments in the first pair of leaves unfolded. Next treatments depending on the occurrence of disease symptoms and pests. The preparations were used alternately.} \\ \hline \multicolumn{5}{c}{Reduced chemical protection with the participation of biopreparations and biostimulants} \\ \hline \hline \multirow{6}{*}{RChP} & \multirow{6}{*}{Topsin M 500 SC} & \multirow{6}{*}{\(1\times\) Comistar Opti} & \(1\times\) Decis Mega 50 EW, 0.151-ha\({}^{-1}\) & \\  & & & \(480\) SC, 2.51-ha\({}^{-1}\) & \(1\times\) Proteus 110 OD, 0.61-ha\({}^{-1}\) & \\  & & & \(200\) g-ha\({}^{-1}\) & \(1\times\) Sumi Alpha 050 EC, 0.21-ha\({}^{-1}\) & \\ \cline{3-5}  & & & \(1\times\) Serenade ASO, 0.21-ha\({}^{-1}\) & \(4\times\) Dipel\({}^{\text{\textregistered}}\) & \\ \cline{3-5}  & & & \(WG\),1 kg-ha\({}^{-1}\) & \\ \cline{3-5}  & & & & \(WG\),1 kg-ha\({}^{-1}\) & \\ \cline{3-5}  & & & & \\ \hline \hline \end{tabular}
\begin{tabular}{c c c} \hline \hline \multicolumn{2}{c}{**Protection Method**} \\ \hline \hline \multirow{6}{*}{Control} & \multirow{6}{*}{Untreated} & \multirow{6}{*}{Without protection} & \(1\times\) Decis Mega 50 EW, 0.151-ha\({}^{-1}\) \\  & & & \(0.1\times\) Proteus 110 OD, 0.61-ha\({}^{-1}\) & \\  & & & \(0.6\)1-ha\({}^{-1}\) & \\ \cline{3-5}  & & & \(1\times\) Sumi Alpha 050 EC, 0.21-ha\({}^{-1}\) & \\ \cline{3-5}  & & & \(4\times\) Dipel\({}^{\text{\textregistered}}\) & \\ \cline{3-5}  & & & \(WG\),1 kg-ha\({}^{-1}\) & \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Scheme of horseradish protection treatments.