## Results and Discussion
### PCA of Spectroscopic Data
#### 3.3.1 PCA Loadings
On the other hand, PC3 loadings demonstrate several positive peaks at 1672 cm\({}^{-1}\) (amide J), 1608 and 1409 cm\({}^{-1}\) (COO' stretching in Asp and Glu),  
\begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{2}{*}{**Method**} & \multicolumn{4}{c}{**Wavenumber (cm\({}^{-1}\))**} & \multirow{2}{*}{**Origin 1**} \\ \cline{2-4}  & & **Loadings** & & & **Operin 1** \\ \cline{2-4}  & **PC1** & **PC2** & **PC3** & & \\ \hline \multirow{8}{*}{FT MIR} & 11697/11619 & 11697/11619 & \(\uparrow\)1633/1614 & 1642 & amide I—proteins, chitin \\  & 115597/11511 & 11556/11511 & 11564/11503 & 1536 & amide II—proteins, chitin \\  & 11472/1744 & 11426/11449 & 14558h & \(\$\)(CHJ), \(\$\)(CHJ) \\  & 11418 & \(\uparrow\)1415 & \(\uparrow\)1418 & 1421 & \(\$\)(CHJ)(COH)—glucans \\  & 11382/11368 & 11379 & 1375 & \(\$\)(CHJ)—proteins, chitin \\  & 11326 & 11326/11299 & 11337/11300 & 1316 & amide III—chitin \\  & 11246 & 11242 & 11245 & 1236 & amide III—proteins \\  & 11204 & \(\downarrow\)1206 & \(\downarrow\)1208 & 1202 & \(\beta\)-p-glucans \\  & 1162/1144 & \(\uparrow\)1163/1142 & \(\uparrow\)1163/1144 & 1150 & \(\$\)(COO)—glucans, chitin \\  & 11115 & \(\downarrow\)1119 & 11114 & 1100 & \(\$\)(COO)—glucans, chitin \\  & 11070 & 11074/11058 & 11074/11058 & 1075 & \(\$\)(CO)(CC)—glucans, chitin \\  & 11031/1982 & 11027/1980 & 11041/1980 & 1040 & \(\$\)(COO)—glucans, chitin \\  & 1944 & \(\uparrow\)943 & \(\uparrow\)946 & 930 & \(\$\)\(\alpha\)-p-glucans \\ \hline \multirow{8}{*}{FT NIR} & 16730, 16638 & 16688/1667634 & 16700 & 6600 sh & NH comb.—proteins, chitin \\  & & & & 16445 & 6470 sh & NH 1\({}^{\rm st}\) ov.—proteins, chitin \\  & & & 15955, 15820 & 15955, 15820 & & CH, CH\({}_{2}\) CH\({}_{3}\) 1 st ov. \\  & & & 15739, 15850 & & CH, CH\({}_{2}\) CH\({}_{3}\) 1 st ov. \\  & \(\$\)5272/15172 & \(\$\)15280/15218 & 15234 & 5165 & OH comb.—glucans, chitin \\  & 15076 & \(\downarrow\)15122 & \(\$\)15134, \(\downarrow\)4999 & & OH comb.—glucans, chitin \\  & 14894/14840 & 14900, 14840 & 14894/14840 & & amide II’ comb.—proteins \\  & 14675/1451 & \(\downarrow\)4675, \(\downarrow\)4567 & \(\$\)4655/14505 & & amide II’ comb.—proteins \\  & 14432, 14378 & 14405 & 14432 & & OH/CO comb.—glucans, chitin \\  & 14231 & \(\uparrow\)4327/14266 & 14335/14266 & 4285 & CH, CH\({}_{2}\) CH\({}_{3}\) comb. \\  & 14173/14088 & 14204/4038 & \(\$\)1450/14081 & & CH, CH\({}_{2}\) CH\({}_{3}\) comb. \\ \hline \multirow{8}{*}{FT Raman} & 11654 & 11670 & 11672 & 1670 & amide I-proteins, chitin \\  & 11610, 11589 & 11591 & 11608 & 1604 & \(\$\)(COO)—proteins \\  & 11535 & & & \(\$\)(C\(\leftarrow\)C)—phenolic compounds \\  & 11444 & \(\downarrow\)1454 & 11448 & 1457 & \(\Figure 2:0\Figure 2:1\)(\(\Figure 2:2\)(\Figure 2:3\))(\(\Figure 2:4\)))))))))))))))))))))))))}}})\(\(())\((((((((((((((((((((((1111))))))))))))))) \\  & & \(\(\uparrow\)1371 & & & \(\Figure 2:5\)(\(\Figure 2:6\)\(\Figure 2:7\)\(\Figure 2:8\)\(\Figure 2:9\)\(\$\)(CHJ), \(\$0\)\(\$\)(CHJ), \(\$1\)\(\$\)(CHJ), \(\$2\$\)(CHJ), \(\$3\)\(\$\)(CHJ), \(\$4\)\(\$\)(CHJ), \(\$5\)\(\$\)(CHJ), \(\$6\)\(\$\)(CHJ), \(\$7\)\(\$\)(CHJ), \(\$8\$\)(CHJ), \(\$9\$\)(CHJ)(COH)—glucans \\  & 11382/11368 & 11379 & 1375 & \(\$0\)\(\$\)(CHJ)(COH)—glucans \\  & 11382/11368 & 11379 & 1375 & \(\$1\$\)(CHJ)(COH)—glucans \\  & 11382/11368 & 11379 & 1375 & \(\$2\$\)(CHJ)(COH)—glucans \\  & 11382/11368 & 11379 & 1375 & \(\$3\$\)(CHJ)(COH)—glucans \\  & 11382/11368 & 11379 & 1375 & \(\$4\)\(\$\1238 cm\({}^{-1}\) (amide III), 1031 and 1004 cm\({}^{-1}\) (The ring vibrations) and 850 and 829 cm\({}^{-1}\) (Tyr doublet); all of them are characteristic for proteins [48; 49].