## 3 Results  
A total of 20 individuals, ranging in age from 18 to 65 years, were enlisted as participants in this study. The findings of the investigation, which assessed the microbiological profiles of patients subjected to two distinct regimens, are outlined in the subsequent paragraphs.  
The mean values of microbiological analysis for each of the analyzed groups, namely Group 1, consisting of patients treated with the LANAP protocol, and Group 2, consisting of patients treated with the OHLLT, are presented in this study. The initial sampling was conducted prior to the implementation of the program, whereas the subsequent sampling occurred one week after the completion of the therapy. The total bacterial count was assessed one week following the initiation of the program. Group 1 exhibited a reduction of 80.3%, while Group 2 showed a reduction of 81.4%. It is noteworthy that both groups exhibited statistically equivalent reductions. The full results and statistics of  
Figure 5: The utilization of a Sioxyl solution for irrigation purposes.  
Figure 6: The last clinical procedure involved utilizing a Wiser diode laser with certain parameters. The laser had a peak power of 2.5 W, a T-On (time on) duration of 20 microns, a T-Off (time off) duration of 80 microns, an average power of 0.5 W, a duty cycle of 100 microns, and a frequency of 10 KHz.  
the microbiological analysis are presented in the previously published paper by the same authors [29].  
The statistical analysis was conducted on the data collected from the questionnaires administered to patients in Group 1, who received therapy using the LANAP protocol, and Group 2, who received treatment using the OHLLT protocol. The analysis revealed the following outcomes.  
Within the first group, the feeling of pain reached its peak at 0 h following the implementation of the LANAP protocol, with an intermediate score of 4.2. Subsequently, there was a gradual decline in pain perception, resulting in an intermediate score of 3.1 after 6 h and ultimately reaching a score of 0 by day 7 (Table 1; Figure 7).  
Within Group 2, the most pronounced sense of pain was observed immediately following the OHLLT treatment, with an intermediate score of 3.5. Subsequently, there was an initial decrease in pain perception to a median score of 1.6 at 6 h, followed by a further fall to 0.1 after 48 h. (Table 1; Figure 8) is referenced in the text. Figure 9 presents a summary of the OHLLT and LANAP clinical protocols.  
The Mann-Whitney test revealed statistically significant differences in the perceived pain levels reported by patients at each recorded instant and the highest pain scores between the two groups. In relation to this matter, the scores showed a notable decrease in Group 2, which consisted of patients who had treatment utilizing the OHLLT regimen.  
\begin{table}
\begin{tabular}{c c c c} \hline
**Time Schedule** & **Median G1 (CI 95\%)** & **Median G2 (CI 95\%)** & **Significance** \\ \hline
0 h & 4.2 (6–3), CI 95\%: 5–3 & 3.5 (5–2), CI 95\%: 4-2 & \(p\) > 0.05 \\
6 h & 3.1 (4–2), CI 95\%: 4-2 & 1.6 (3–1), CI 95\%: 2–1 & \(p\) < 0.05 \\
12 h & 2.3 (3–1), CI 95\%: 3–1 & 0.9 (2–0), CI 95\%: 1–0 & \(p\) < 0.05 \\
24 h & 1.8 (3–1), CI 95\%: 2–1 & 0.4 (2–0), CI 95\%: 1–0 & \(p\) < 0.05 \\
48 h & 0.9 (2–0), CI 95\%: 1–0 & 0.1 (1–0), CI 95\%: 0–0 & \(p\) < 0.05 \\
7 days & 0 (0–0), CI 95\%: 0–0 & 0 (0–0), CI 95\%: 0–0 & - \\ \hline \end{tabular}
\end{table}
Table 1: The study aimed to determine the pain experienced at each time schedule using the Numeric Rating Scale (NRS). The participants were divided into two groups: Group 1 (G1) and Group 2 (G2). The analysis was conducted with a 95% confidence interval (CI). The _p_-value was defined as being less than or equal to 0.05 and was evaluated using the Mann–Whitney test.  
Figure 7: A graphical representation illustrating the temporal evolution of pain intensity in Group 1 and Group 2 (median values for groups of patients).