## 4 Discussion and Conclusions
Mycelium acted as the primary degradable component, with weight loss of up to 43% observed in samples after sixteen weeks in potting soil [44]. While this methodology was applied to a specific fungal strain and substrate, it presents a relevant approach that could be adapted to assess the degradation behavior of the material developed in this study. The successful integration of the formulated paste optimized robotic EAM parameters, and toolpath planning parameters in this study provides a foundation for the designing and fabricating acoustic panels using living mycelium-based mixtures. While prior studies focused on paste formulation and extrusion feasibility of MBCs [12; 13; 16], this study advances both the paste formulation and the EAM workflow. The paste is developed using ground Ecovative's Dehydrated Grow-It-Yourself Mushroom(r) Material, with inoculation initiated prior to EAM. This eliminates the need for a separate colonization step in growth bags, enabling direct EAM of the living material and significantly reducing the total fabrication timeline. Additionally, this study uses a data-driven strategy using an Artificial Neural Network (ANN) for optimizing the robotic EAM parameters for mycelium-based mixtures, which can be extended to future formulations for consistent and predictable extrusion. A hybrid slicing approach combining planar and non-planar slicing methods improves surface quality and enables fabrication of conformal geometries. Finally, this study uses robotic EAM for the fabrication of architectural-scale acoustic panels, demonstrating its potential for fabrication of performance-based building parts beyond conventional mold-based methods. While this study focused on panel-scale fabrication and acoustic performance, future work should address installation methods and mounting details. Studying boundary conditions of the panels, joinery, and support structures will be necessary to minimize acoustic bridging. Acoustic bridge refers to a structural sound transmission path that couples with the airborne sound pathway and reduces overall sound transmission loss [45]. Beyond acoustic panel fabrication, this research addresses the growing need for precise and efficient fabrication solutions for biomaterials, ultimately contributing to more sustainable material development. Future studies could investigate the long-term durability and biodegradability of MBC-based acoustic panels to expand their use in architecture. Additionally, broadening the methodology to explore various paste formulations, geometries, and toolpath planning strategies would enable application-specific designs that meet an even wider range of performance requirements. Conceptualization, A.M., O.B.C., A.Z., N.W. and B.G. ; methodology, A.M., O.B.C., A.Z., N.W. and B.G. ; software, A.M., O.B.C. and N.W. ; validation, A.M., O.B.C., N.W. and B.G. ; formal analysis, A.M., O.B.C. and N.W. ; investigation, A.M., O.B.C. and N.W. ; resources, B.G. ; data curation, A.M., O.B.C. and N.W. ; writing--original draft preparation, A.M., O.B.C. and N.W. ; writing--review and editing, B.G. ; visualization, A.M., O.B.C. A.Z. and N.W. ; supervision, B.G. ; project administration, B.G. ; funding acquisition, B.G. All authors have read and agreed to the published version of the manuscript. **Funding:** This research is supported by the Stuckeman Center for Design Computing Research Fund at the Pennsylvania State University. **Institutional Review Board Statement:** Not applicable. **Informed Consent Statement:** Not applicable. **Data Availability Statement:** The original contributions presented in the study are included in the article, further inquiries can be directed to the corresponding author. **Acknowledgments:** The authors acknowledge the use of the facilities at the Mushroom Research Center, Penn State University, during the course of this work.