## 3 Results and Discussion
### HPLC-PDA-tQ-ESI-MS Profiles of SPE Fractions of Silene repens: Qualitative Study
repens_ seedlings did not indicate statistically significant differences compared to control samples. The concentrations of 20-hydroxyecdysone and polypodine B in leaves of _S. repens_ seedlings slightly increased (0.95\(\rightarrow\)1.05 mg/g and 0.39\(\rightarrow\)0.47 mg/g, respectively) after the treatment with indole-3-butyric acid (100 mg/L) compared with the control. The content of 20-hydroxyecdysone in roots also slightly increased from 0.42 to 0.50 mg/g compared to the control. Indole-3-butyric acid is representative of the auxin  
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Group**} & \multirow{2}{*}{ \begin{tabular}{c} **Concentration,** \\ **mg/L** \\ \end{tabular}  } & \multicolumn{3}{c}{**Leaves**} & \multicolumn{2}{c}{**Roots**} \\ \cline{3-6}  & & **Weight,** \(\mathrm{\textsc{\textsc{\textsc{\textsc{\textsc{\textsc{\textsc{\textsc{\textsc{\textsc{\textsc{\textsc{\textsc{\textsc{\  
class of phytohormones and as a derivative of indole stimulates the growth of plant fruits and shoots, causes positive geotropism of roots, affects cell differentiation, and ensures the interaction of individual organs [46; 47]. Spraying of _S. repens_ seedlings with 4-chlorophenylaphenylacetic acid (100 mg/L), which is similar to auxins in chemical structure, led to an increase in the productivity of both leaves (60.0\(\rightarrow\)79.6 mg) and roots (51.2\(\rightarrow\)61.6 mg) of _S. repens_ seedlings. The content of 20-hydroxycosone in _S. repens_ leaves increased from 0.97 to 1.06 mg/g and decreased in roots from 0.44 to 0.38 mg/g. The content of polypodine B in all groups did not indicate a statistically significant difference compared to the control sample. 4-Chlorophenoxylactic acid is used in agriculture to increase the productivity of species by stimulating the formation of ovaries and preventing them from dropping [48; 49]. It is likely that the short duration of the experiment and the absence of generative organs in _S. repens_ seedlings did not allow to fully assess the effect of this growth regulator. Gibberellic acid potassium salt at maximum concentration showed the increased productivity of _S. repens_ leaves by 1.74 times (from 60.0 to 104.3 mg) and roots by 1.42 times (from 51.2 to 72.5 mg). Despite the increase in productivity, the content of 20-hydroxycosone in leaves increased slightly from 0.97 to 1.08 mg/g. Concentration of polypodine B did not indicate a statistically significant difference compared to the control sample. However, the content of 20-hydroxycosone in roots of _S. repens_ seedlings increased from 0.44 to 0.66 mg/g. Previously, the synergistic effect of 20-hydroxycosone and gibberellic acid was noted for dwarf rice in an increase in the length of seedlings [43]. Gibberellins are phytohormones with a tetracycline structure and are associated with the stimulation of vegetative and generative developments of plants [50]. Gibberellins work in the same direction as auxins and stimulate biosynthesis and signal transmission of each other [51]. Treatment with elicitor arachidonic acid and its ether led to a significant increase in productivity of _S. repens_ seedlings. It was revealed that the maximum yield of _S. repens_ leaves (60.0\(\rightarrow\)113.3 mg) and roots (51.2\(\rightarrow\)73.2 mg) comparing all experimental groups was observed after spraying with ethyl arachid (100 mg/L). However, the content of 20-hydroxycosone and polypodine B in _S. repens_ leaves and roots was lower than in the control samples when treated with arachidonic acid and ethyl arachid (both 100 mg/L). Thus, if it is necessary to increase the yield of _S. repens_, spraying seedlings with ethyl arachid (100 mg/L) is justified. However, if it is required to obtain _S. repens_ seedlings with high content of ecdysteroids, spraying seedlings with epibrassinolide (100 mg/L) is recommended. Effect of Phytolormones and Elicitors on Content of Glycos/flavones in Introduced Seedlings of _S.