# A New Approach for the Utilization of Technical Egg Albumen Based on Acid-Thermal Coagulation
## 3. Results
### Characterization and Changes During Storage of Dried Technical Albumen Products
#### 3.3.5 Changes in Microbial Status During Storage  
A similar initial (\(p\geq 0.05\)) total number of mesophilic aerobic microorganisms, expressed by the index total microbial number mentioned in Table 8, was found in samples CA, PA, and CADF. In sample PADF, the highest (\(p\leq 0.05\)) degree of microbial insemination was evaluated.  
After a month of storage, a difference was found in the PADF sample, whose number of mesophilic aerobic microorganisms decreased (\(p\leq 0.05\)) compared to the initial one. The remaining three samples remained stable (\(p\geq 0.05\)) relative to their baseline values. At the same time, the difference between the four samples is statistically insignificant (\(p\geq 0.05\)).  
The detected number of coliforms was the lowest in sample PA (\(p\leq 0.05\)). CA and CADF samples, as well as PADF, had statistically insignificant differences (\(p\geq 0.05\)). No presence of _E. coli_ was detected in all the tested samples. Microbiological analysis of the  
Figure 5: Changes in TBARS, mg MDA/kg of the dried egg products from technical album. Results are presented as means \(\pm\) SEM; superscripts \({}^{\text{a,b,c}}\) indicate significant (\(p\leq 0.05\)) differences between the dried technical albumen products for each parameter separately, while superscripts \({}^{\text{x,y}}\) indicate significant (\(p\leq 0.05\)) differences between time periods.  
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**CFU/g**} & \multirow{2}{*}{**CA**} & \multirow{2}{*}{**CADF**} & \multirow{2}{*}{**PA**} & \multirow{2}{*}{**PADF**} & \multirow{2}{*}{**\(p\)-Value/ Significance F**} \\ \hline \multicolumn{6}{c}{24 h after production} \\ \hline TPC & 4.92 \({}^{\text{ax}}\) \(\pm\) 0.47 & 4.73 \({}^{\text{ax}}\) \(\pm\) 1.33 & 4.81 \({}^{\text{ax}}\) \(\pm\) 0.39 & 5.37 \({}^{\text{ax}}\) \(\pm\) 0.35 & 0.808425 \\ Coliforms & 4.00 \({}^{\text{a}}\) \(\pm\) 1.00 & 3.00 \({}^{\text{b}}\) \(\pm\) 0.420 & 4.15 \({}^{\text{a}}\) \(\pm\) 1.15 & 3.83 \({}^{\text{a}}\) \(\pm\) 0.62 & 0.768197 \\ _E. coli_ & N.F. & N.F. & N.F. & N.F. & - \\ _Enterobacteriaceae_ & \textless{10} & \textless{10} & \textless{10} & \textless{10} & \textless{10} & - \\ _Salmonella_ spp. & N.F. & N.F. & N.F. & N.F. & - \\ \hline \hline \multicolumn{6}{c}{1 month after production} \\ \hline TPC & 3.96 \({}^{\text{ax}}\) \(\pm\) 0.41 & 4.65 \({}^{\text{ax}}\) \(\pm\) 0.37 & 4.41 \({}^{\text{ax}}\) \(\pm\) 0.37 & 4.40 \({}^{\text{ax}}\) \(\pm\) 0.38 & 0.1670 \\ Coliforms & N.F. & N.F. & N.F. & N.F. & \\ _E. coli_ & N.F. & N.F. & N.F. & N.F. & - \\ _Enterobacteriaceae_ & \textless{10} & \textless{10} & \textless{10} & \textless{10} & - \\ _Salmonella_ spp. & N.F. & N.F. & N.F. & N.F. & - \\ \hline \hline \end{tabular} Results are presented as means \(\pm\) SEM; superscripts \({}^{\text{a,b}}\) indicate significant (\(p\leq 0.05\)) differences between the dried technical albumen products for each parameter separately, while superscripts \({}^{\text{x}}\) indicate significant (\(p\leq 0.05\)) differences between time periods. N.F.—Not Found.  
\end{table}
Table 8: Changes in microbiological status of the dried egg products from technical albumen during storage.  
four samples stored for one month did not detect the presence of both coliforms and _E. coli_. This is probably due to the low water activity resulting from moisture loss during storage.  
It was found that in all investigated samples, the number of _Enterobacteriaceae_ microorganisms was below 10 CFU/g (\(p\geq 0.05\)), both at the beginning of the experiment and at the 1 st month of storage (Table 8).  
The examination of the dry egg products from technical protein did not detect the presence of _Salmonella_ spp. for the entire period of the experiment.