## 3 Results  
### Antioxidant Parameter Activities in H. salsolifolium Extracts  
A calibration curve (y = \(-\)0.0164x + 1.1767, R2 = 0.9916 \(\mathrm{\SIUnitSymbolMicro m}\)/mL) was drawn using Trolox for the DPPH assay and the antioxidant activity was calculated using this curve (mg Trolox/g). The highest activity values in the extracts were determined as methanol > hexane > water > dichloromethane (66.14 \(\pm\) 3.30, 65.4 \(\pm\) 3.27, 65.16 \(\pm\) 3.25, and 59.61 \(\pm\) 2.98, respectively). \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Cell Lineage** & **Cancer-Normal** & **Histopathology** & **Culture** \\ \hline A549 & Lung Cancer Epithelium & Cancer & RPMI-1640 \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Cancer cell line used in the study. A calibration curve (y = \(-\)0.0147x + 1.1707, R2 = 0.99 \(\mathrm{\SIUnitSymbolMicro\mathrm{g}}\)/mL) was drawn using Trolox for the ABTS assay and the antioxidant activity was calculated using this curve (mg Trolox/g). The highest activity values in the extracts were determined as water > methanol > dichloromethane > hexane (75.01 \(\pm\) 3.75, 73.38 \(\pm\) 3.66, 32.42 \(\pm\) 1.62, and 11.2 \(\pm\) 0.56, respectively). A calibration curve (y = 0.0056x + 0.053, R2 = 0.9942 \(\mathrm{\SIUnitSymbolMicro\mathrm{g}}\)/mL) was drawn using Trolox for the FRAP assay and the antioxidant activity was calculated using this curve (mg Trolox/g). The highest activity values in the extracts were determined as methanol > water > dichloromethane > hexane (322.14 \(\pm\) 16.10, 308.21 \(\pm\) 15.41, 136.42 \(\pm\) 6.82, and 37.32 \(\pm\) 1.86, respectively). A calibration curve (y = 0.0293x + 0.0572, R2 = 0.9911 \(\mathrm{\SIUnitSymbolMicro\mathrm{g}}\)/mL) was drawn using Trolox for the CUPRAC assay and the antioxidant activity was calculated using this curve (mg Trolox/g). The highest activity values in the extracts were determined as water > dichloromethane > methanol > hexane (87.94 \(\pm\) 4.39, 12.41 \(\pm\) 0.62, 10.5 \(\pm\) 0.52, and 2.65 \(\pm\) 0.13, respectively). The TPC values of the _H. salsolifolium_ plant extracts were examined in the study. A calibration curve (y = 0.0144x + 0.0728, R2 = 0.9952 \(\mathrm{\SIUnitSymbolMicro\mathrm{g}}\)/mL) was drawn by calculating gallic acid, and the total phenolic amount was calculated using this curve. The results were expressed as mg GAE/g. The highest activity values in the extracts were determined as methanol > water > dichloromethane > hexane (68.9 \(\pm\) 3.44, 64.52 \(\pm\) 3.22, 7.51 \(\pm\) 0.37, and 3.68 \(\pm\) 0.14, respectively). In the examination of the TPC values of the _H. salsolifolium_ plant extracts, a calibration curve (y = 0.0024x + 0.0344, R2 = 0.9957 \(\mathrm{\SIUnitSymbolMicro\mathrm{g}}\)/mL) was drawn by calculating quercetin and the total flavonoid amount was calculated using this curve. The results were expressed as mg QE/g. The highest activity values in the extracts were determined as methanol > water > dichloromethane > hexane (104.69 \(\pm\) 5.23, 83.72 \(\pm\) 4.18, 13.02 \(\pm\) 0.65, and 6.08 \(\pm\) 0.30, respectively). The antioxidant activities of the different fractions of the _H. salsolifolium_ extracts were examined by the ABTS, DPPH, CUPRAC, TPC, and TFC assays and the results are given in Table 2. It was seen that the antioxidant activities and TPC and TFC values of the methanolic and aqueous extracts showing polar properties were higher than those of the other nonpolar extracts. Significantly positive correlations were determined between the TPC values and the ABTS and FRAP values (\(r\) = 0.97, \(p\) = 0.015; \(r\) = 0.968, \(p\) = 0.016; respectively) (Table 3). Significantly positive correlations were determined between the TFC values and the ABTS and FRAP values (\(r\) = 0.957, \(p\) = 0.022; \(r\) = 0.963, \(p\) = 0.018; respectively).