## 1 Introduction  
Daucosterol [(36)-stigmast-5-en-3-yl \(\beta\)-D-glucopyranoside], a natural \(\beta\)-sitosterol glucose, is a saponin phytosterol belonging to different families and genera found in several countries such as China, Vietnam, Mexico, Algeria, Tunisia, Italy, Iran, Cameroon, Saudi Arabia, Nigeria, Korea, and India [1; 2; 3; 4]. Daucosterol is the major component of several plant extracts, namely Chinese _Fallopia cillinerve_ root extract [1], _Dendrobium huoshanense_ and _Dendrobium officinal_ stem extract [5], and _Hyssopus cuspidatus_ Boriss aerial part extract [6]. In addition, this natural agent was found to be the main component of aerial part extracts of _Centaurea resupinata_ subsp. _dufourii_[7] and _Oronis mitissina_ L. [8] from Algeria. It was also detected at high concentrations in leaf and stem extracts of _Prangos ferulacea_ from Iran [9], aerial part extracts of _Cassia italic_ collected from Saudi Arabia [3], and leaf extracts of _Ficus deltaidea_ from Indonesia [10] and _Dioscorea batatas_ collected from Korea [11]. Furthermore, daucosterol is the principal component of aerial part extracts of _Astragalus tanae_ found in Italy [12] and _Landolphia owaraensis_ collected from Nigeria [13], as well as whole plant and root extracts of _Rheum turkestanicum_ from Iran [14; 15]. Other investigations have reported high contents of daucosterol in plant extracts, namely _Archidendron clyperaria_ harvested in Vietnam [16] and _Paraseneico pseudoatimgasa_ leaf extract [17] and _Grewia optiva_ [PERSON] stem bark extract in Pakistan [18]. Concerning the purification and isolation of daucosterol from plants, several methods have been used as spectroscopic techniques, including NMR and FT-IR, on the stems and leaves of _Prangos ferulacea_[9] and the aerial parts of _Cassia italica_[3] and _Oronis mitissina_ L. [8]. NMR (\({}^{1}\)H NMR, \({}^{13}\)C NMR, COSY, HSQC, and HMBC) and mass spectroscopy (ESI-MS) have been used to elucidate daucosterol in _Centaurea resupinata_ subsp. _[PERSON]_[7]. This molecule has been elucidated from _Dioscorea opposite_ with silica gel column chromatography (SGCC) using CHCl3-MeOH [19], from _Phylenthus emblica_ L. with thin layer chromatography (TLC) [20], from _Portulaca olerca_ L. [21] and _Eriobotrya fragrans_ Champ [22] with \({}^{1}\)H and \({}^{13}\)C aided by HMQC, and from _Arcttoids arcttooides_[23] using NMR (COSY, NOESY, HMQC, and HMBC) and mass spectra. Several studies have highlighted the pharmacological properties of daucosterol, including chemopre [24; 25; 26], neuroprotective [27; 28; 29; 30], antioxidant [7; 8; 9], anti-inflammatory [31], antidiabetic [32], inhibition and interactions of alpha-amylase by daucosterol from the peel of Chinese water chestnut [33], and immunomodulatory [34]. Immunoregulatory activity by daucosterol, a \(\beta\)-sitosterol glycoside, induces a protective Th1 immune response against disseminated Candidiasis in mice [35]. Regarding its chemopre potential, daucosterol was found to possess important anticancer activity on various tumor cell lines and could be considered as one of the novel pharmacological treatment strategies for cancer: for breast adenocarcinoma through different cellular and molecular mechanisms, such as an increase in pro-apoptotic protein Bax and Bcl2, a decrease in the Bcl-2/Bax ratio, upregulation of the PTEN gene, inhibition of the PI3K/Akt pathway, loss of mitochondrial membrane potential and cytochrome c (Cyt c) [36; 37], repression of cell migration and invasion, and induction of cell death by cell-cycle arrest and apoptosis [38; 39]; for lung cancer by increasing reactive oxygen species (ROS) level and promoting intrinsic apoptotic cell death on A549 cells mediated by increased expression of caspase-3, caspase-9, Bax, PARP inactivation, Cyt c release, and diminished bcl-2 protein expression; and for hepatocellular carcinoma by inhibiting the proliferation, migration, and invasion of hepatocellular carcinoma cells via Wnt/\(\beta\)-Ccatenin signaling [40].