## 3 Results and Discussion
### Bacterial Characterization
#### 3.1.2 Biolog
\begin{table}
\begin{tabular}{c c c c c c c} \hline
**Type** & **Substrate** & **AN** & **Gen III** & **Type** & **Substrate** & **AN** & **Gen III** \\ \hline Amine/Amide & Alaninamide & 78 & N/A & Carbohydrate & Sucrose & 100 & 87 \\ Glucuronamide & N/A & 85 & D-Trehalose & 100 & 81 \\  & Sucminic Acid & 0 & N/A & & Turanose & 88 & 87 \\ Amino Acid & L-Alanine & 100 & 93 & D-Galacturonic acid & 100 & 93 \\  & L-Alanyl-L-Glutamine & 100 & N/A & D-Gluconic acid & 100 & 94 \\  & L-Alanyl-L-histidine & 66 & N/A & Glucuronic Acid & N/A & 93 \\  & L-Alanyl-L-threonine & 100 & N/A & D-Glucosamine acid & 0 & N/A \\  & L-Ar Argin & N/A & 78 & Acetic acid & 38 & 87 \\  & L-Apargazine & 78 & N/A & Formic acid & 0 & 82 \\  & D-Aspartic Acid & N/A & 73 & Fumaric acid & 78 & N/A \\  & L-Histidine & N/A & 89 & Glyoxylic acid & 0 & N/A \\  & L-Glutamic acid & 82 & 93 & \(\alpha\)-Hydroxybutric acid & 0 & 13 \\  & L-Clutamine & 100 & N/A & \(\beta\)-Hydroxybutric acid & 24 & 88 \\ Glyoxyl-L-aspartic acid & 72 & N/A & & Iaconic acid & 0 & N/A \\ Glyoxyl-L-glutamine & 100 & N/A & & \(\alpha\)-Ketobutyric acid & 0 & 2 \\  & Glyoxyl-L-methionine & 0 & N/A & & \(\alpha\)-Ketobacter acid & 0 & N/A \\ Glyoxyl-L-proline & 100 & 94 & Carboxylic acid & a-Ketoglutaric acid & N/A & 60 \\  & L-Methionine & 0 & N/A & D-Lactic acid & 60 & N/A \\  & L-Phenylalanine & 0 & N/A & L-Lactic acid & 100 & 94 \\  & L-pyroglutamic acid & N/A & 79 & D-Malic acid & 0 & 68 \\  & D-Serine & N/A & 10 & L-Malic acid & 100 & 95 \\  & L-Serine & 100 & 94 & Prognionic acid & 0 & 5 \\  & L-Threonine & 0 & N/A & Pyrruvic acid & 100 & N/A \\  & L-Valine & 0 & N/A & D-Saccharic acid & 100 & 94 \\  & L-Valine + L-aspartic acid & 100 & N/A & Succinic acid & 100 & N/A \\ N-Acetly-D-galactosamine & 0 & 44 & m-Tartaric acid & 100 & N/A \\  & N-Acetyl-D-glucosamine & 100 & 94 & Mucic Acid & N/A & 93 \\  & N-Acetly-D-mannosamine & 100 & 94 & Quinic Acid & N/A & 68 \\  & Adomitol & 0 & N/A & Citric Acid & N/A & 94 \\  & D-Arabitol & 0 & 67 & Acetoacetic Acid & N/A & 55 \\  & Arbutin & 82 & N/A & Amygdala & 0 & N/A \\  & D-Cellobiose & 100 & 92 & Glycerol & 24 & 87 \\  & Dulcitol & 16 & N/A & Phosphate & 100 & N/A \\ Erythritol & 0 & N/A & Glucose-1-phosphate & 100 & N/A \\  & D-Fructose & 100 & 85 & Glucose-6-phosphate & 100 & 95 \\  & D-Fucose & N/A & 45 & Fructose-6-phosphate & N/A & 93 \\  & L-Fucose & 0 & 67 & D-Salicin & 78 & 85 \\  & D-Galactose & 100 & 84 & D,L-Lactic acid & 0 & 78 \\  & Gentiobiose & 78 & 88 & Myruvic acid & 100 & 89 \\ Carbohydrate & & & & & & \\  & & & & & & \\  & & & & & & \\  & & & & & & \\  & & & & & & \\ \end{tabular}
\end{table} 
Table 1: Carbon source utilization pattern of isolate KBH 6958 using BIOLOG AN and GEN III plates. Carbon sources on both microplates can be classified into six groups based on their functional group. The six groups were amine/amide, amino acid, carbohydrate, carboxylic acid, polymers, and other miscellaneous carbon sources. The substrate utilization patterns appeared random at first glance, with no discernible correlations with the respective substrate groups. The level of carbon utilization was determined by the individual substrate rather than the group type. In general, common monosaccharides such as \(\alpha\)-D-glucose, \(\alpha\)-D-fructose, \(\alpha\)-D-galactose, and \(\alpha\)-D-lactose were very favorable to this isolate in both aerobic and anaerobic conditions (utilisation level nearly 100%). However, some carbon sources, such as \(\alpha\)-ketobutyric acid and \(\alpha\)-hydroxybutyrate acid, were unable to sustain KBH 6958 growth because they were not utilised (the utilisation level was nearly 0%) in both plates. These results also highlighted the difference of metabolism in aerobic and anaerobic conditions. In most of the carbon sources found in both Gen III and AN microplates, the availability of oxygen positively affects the level of substrate utilization.