## 3 Results
### Three Months Observations and Overall Survival Post IRE  
**Case 1** (IRE with intratumoral administration of calcium ions). CT and MRI images of the patient are presented in Figure 2A,B. In the follow-up CT examination, the size of the pancreatic tumor was found to be reduced, with features of internal lysis. The presence of air inside the tumor was related to a fistula between the pancreatic ducts and the tumor, and inflammation was also detected. \begin{table}
\begin{tabular}{c c c c} \hline  & **Case 1 (IRE with CaCl\({}_{2}\)** & **Case 1 (IRE with CaCl\({}_{2}\)** & **Case 2 (IRE with Chemotherapy and CaCl\({}_{2}\) Administered after the Procedure)** & **Case 2 (IRE with Chemotherapy and CaCl\({}_{2}\) Administered after the Procedure)** \\ \hline Voltage & 1.09-1.2 kV & 2.8–1.35 kV & 1.05-1.47 kV \\ \hline Electrode type & Single & Single & Single \\ \hline Electrode spacing & 1.1-1.9 cm & 1.1–1.9 cm & 1.2–1.4 cm \\ \hline Number of pulses per electrode pair & 10 & 10 & 10 \\ \hline Number of electrode replacement & None & None & 1 \\ \hline Operating time & 2 h 50 min & 3 h 10 min \\ \hline Anesthesia & Epidural and general anesthesia & Epidural and general anesthesia \\ \hline Administered additional drugs & Calcium ions intratumoral before the procedure & Calcium ions intratumoral after the procedure Cisplatin intravenous before the procedure \\ \hline \end{tabular}
\end{table} 
Table 2: Electroporation protocols, anesthesia, and additional drug data. Figure 2: Patients’ imaging after the IRE procedure: (**A**) Case 1. In the follow-up CT examination, 3 months after electroporation, a slight reduction in the size of the pancreatic tumor was found, with the presence of features of internal lysis. The presence of air inside the tumor was related to fistula between the pancreatic ducts and the tumor, and inflammation was also found; (**B**) Case 1. In the follow-up MRI examination (T1 images, contrast-dependent) 3 months after the electroporation procedure, an area of necrosis lysis in the tumor (no enhancement and low signal) was found, (**C**) Case 2. The CT examination after the administration of the contrast agent showed necrosis of the tumor (visible in the image). In the follow-up MRI (T1 images, contrast-dependent), a necrotic area in the tumor was also found. However, the patient was discharged in good condition and referred for further CTH. The patient was admitted for follow-up examination after the ablation procedure. After the procedure, he did not receive CTH due to persistent pancreatic fistula. The drain was removed, and the patient's WHO performance status was I. He did not report impaired food intake; however, he did report appetite disorders, which was most likely related to acute pancreatitis, immediately after the procedure. A further decrease in body weight to 75 kg (8 kg since the procedure) occurred; however, at present, the patient does not report further weight loss. The patient periodically takes analgesics (NSAIDs), and the VAS level periodically equals to 1. The Ca 19-9 level was 408 U/L. The patient was discharged in good condition and referred for further CTH. Unfortunately, the patient did not respond well to the chemotherapy. The patient refused treatment after another 3 months and died after 9 months from diagnosis and 6 months from calcium electroporation. **Case 2** (IRE with intratumoral administration of calcium ions and intraoperative chemotherapy). CT image of the patient after the procedure is presented in Figure 2C. In a short time after IRE-Ca administration, the patient continued with CTH (for 2 months). The pancreatic fistula had ceased to be active. Moreover, the patient gained 5 kg weight, and the VAS score was 1. The patient has stopped the intake of opioids, and his quality of life is currently significantly better. After 21 months from diagnosis and 12 months from calcium electroporation and electrochemotherapy, the patient is well. He gained another 15 kg, has no fistula sign, and has no pain. He continued CTH, but to date has had a 2-month break.