###### Abstract  
The chemical profile of the _Cannabis sativa_ L. female inflorescences is rather complex being characterized by a large number of molecules belonging to different chemical classes. Considering the numerous applications in various fields, including the medical and pharmaceutical sectors, that have seen a large use of _Cannabis_ genus in recent years, a precise characterization of the matrices is essential. In this regard, the application of adequate and suitable sampling and analysis techniques becomes important in order to provide an identification of the metabolites characterizing the profile of the sample under examination. The goal of this work is to provide additional information on the chemical composition of the inflorescences of five C. _sativa_ different cultivars grown in Emilia Romagna (Italy) through the application of sophisticated analysis techniques such as Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry and Ultra-Performance Liquid Chromatography-Mass Spectrometry (SPME-GC-MS and UPLC-MS). The obtained data highlighted the presence of a high number of volatile and non-volatile compounds, thus allowing a comparative evaluation of the different samples. Furthermore, an in-depth statistical survey by Principal Components Analysis (PCA) and HeatMap, Hierarchical luster Analysis (HCA) and Partial Least Squares Discriminant Analysis (PLS-DA-VIP), was conducted to consider any correlations between the investigated cultivars. The findings of this study may help to provide more information on the _C. sativa_ inflorescences useful for potential applications of their metabolites in scientific research. cannabinoids; non-cannabinoids; volatile and non-volatile compounds; chromatographic analyses; multivariate statistical analysis +
Footnote †: _Appl. still commonly eaten, roasted or raw [6; 7]. In recent years, the renewed interest in the therapeutic effects of _C. sativa_ has led to the legitimate medicinal use through clinical studies demonstrating its efficacy [8]. Moreover, currently, _C. sativa_ is an agricultural commodity grown to be used in the production of foods and beverages, nutritional supplements, cosmetics and personal care products, textiles, paper, insulation materials, and other manufactured goods [9; 10]. From a chemical point of view, _C. sativa_ is a complex species with numerous (>500) reported secondary metabolites, both cannabinoid and non-cannabinoid constituents, obtained from all plant parts (leaves, flowers, bark, seeds, and roots) [11; 12; 13; 14]. The former are a specific chemical class found in _Cannabis_ genus, a group of compounds with a characteristic C21 terpenophenolic backbone and divided into several sub-classes [15]. The latter belong to various chemical classes including alkaloids, flavonoids, non-cannabinoid phenols, and terpenes as well as others [12; 15; 16; 17]. While the stems provide cellulosic and woody fibers and the seeds are exploited in the feed and food industry for their high content of fatty acids and proteins, the leaves and infloresences of _C. sativa_ are a rich source of phytochemicals [18]. In this work, the infloresences of five organic _C. sativa_ commercial cultivars were chemically investigated. In particular, (i) V1 CBD, (ii) Banana Hybrid, (iii) Green Poison (iv), Candy BUD and (v) Gorilla CBD (Figure 1) were analyzed, all with a high cannabinoid content and characterized by medium/large sized buds, compact and containing a good amount of resin. They are obtained from greenhouse crops located in the Tuscan-Emilian Apennines (Italy), where the entire processing cycle is carried out strictly by hand and the tanning process is particularly long in order to obtain and enhance their unique taste. In detail, Green Poison gives off an unusual smell due to contrasting sugary and bitter aromas leaving a very pleasant aftertaste. As the name suggests, Banana Hybrid releases sweet banana fragrances. Both V1 CBD and Candy BUD have a dense and particularly fragrant smell with fruity notes reminiscent of citrus fruits.