## 6 Limitations and Future Work  
In this study, we carried out our experiments on culture cells, and we used one concentration of treatment-100 \(\upmu\)M, which, in our previous studies, has demonstrated significant differences between treated and untreated cells. Future transcriptomic studies might be dose-dependent and include in vivo experiments as well as testing other products of lipid peroxidation.  
Supplementary materials can be accessed at: [[https://www.mdpi.com/2076-3417/11/6/2678/s1](https://www.mdpi.com/2076-3417/11/6/2678/s1)]([https://www.mdpi.com/2076-3417/11/6/2678/s1](https://www.mdpi.com/2076-3417/11/6/2678/s1)). Figure S1. Leucomethylene blue (LMB) assay. Figure S2: Principal component analysis (PCA), Figure S3: Sample-to-sample distance heatmaps generated by DESeq2, Figure S4: MA plots of differentially expressed genes (DEGs) between untreated and treated PDiff Caco-2 cells, Figure S5: Quantitative RT-PCR validation of RNA-seq data in 13-HPDDE-treated PDiff Caco-2 cells, Figure S6: Quantitative RT-PCR validation of RNA-seq data in LA-treated PDiff Caco-2 cells, Figure S7: Quantitative RT-PCR validation of RNA-seq data in H\({}_{2}\)O\({}_{2}\)-treated PDiff Caco-2 cells, Table S1: Primer sequences, Table S2: Number of sequence reads per sample after trimming, Table S3: Differential gene expression in 13-HPDDE-treated cells, Table S4: Gene Ontology analysis (biological processes) in 13-HPDDE-treated PDiff Caco-2 cells, Table S5: Gene Ontology analysis (molecular functions) in 13-HPDDE-treated PDiff Caco-2 cells, Table S6: Gene Ontology analysis (cellular components) in 13-HPDDE-treated PDiff Caco-2 cells, Table S7: Differential gene expression in LA-treated cells, Table S8: Gene Ontology analysis (biological processes) in LA-treated PDiff Caco-2 cells, Table S9: Gene Ontology analysis (molecular functions) in LA-treated PDiff Caco-2 cells, Table S10: Gene Ontology analysis (cellular components) in LA-treated PDiff Caco-2 cells, Table S11: Differential gene expression in H\({}_{2}\)O\({}_{2}\)-treated cells, Table S12: Gene Ontology analysis (biological processes) in H\({}_{2}\)O\({}_{2}\)-treated PDiff Caco-2 cells, Table S13: Gene Ontology analysis (molecular functions) in H\({}_{2}\)O\({}_{2}\)-treated PDiff Caco-2 cells, Table S14: Gene Ontology analysis (cellular components) in H\({}_{2}\)O\({}_{2}\)-treated PDiff Caco-2 cells.  
Figure 7: A summary of the cellular events and processes altered in PDiff Caco-2 cells by 13-HPDDE, LA, or H\({}_{2}\)O\({}_{2}\).  
**Author Contributions:** All authors have contributed substantially to the work reported. Conceptualization, N.F., S.Y., and S.P.; data curation, N.F. and S.Y.; formal analysis, N.F. and S.Y.; funding acquisition, S.Y. and S.P.; investigation, N.F., C.A.N., A.F., and S.Y.; methodology, N.F., C.A.N., A.F., S.Y., and S.P.; project administration, N.F., C.A.N., A.F., S.Y., and S.P.; resources, N.F., C.A.N., A.F., S.Y., and S.P.; software, N.F. and S.Y.; supervision, N.F., A.F., S.Y., and S.P.; validation, N.F., S.Y., and S.P.; visualization, N.F., S.Y., and S.P.; writing--original draft, N.F., S.Y., and S.P.; writing--review and editing, N.F., C.A.N., A.F., S.Y., and S.P. All authors have read and agreed to the published version of the manuscript.  
This research received no external funding.  
Not applicable.  
**Data Availability Statement:** All sequence files and metadata are available at NCBI under BioProject accession PRJNA694700. [[https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA694700](https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA694700)]([https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA694700](https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA694700)) (accessed on 25 January 2021).  
The authors declare no conflict of interest.