## 2 Materials and Methods
### Sequence Alignment and Phylogenetic Analysis  
A dataset consisting of nrSSU, nrITS, nrLSU, and RPB1 was assembled based on BLASTn results and of the most recent phylogenetic studies focused on Halosphaeriaceae and _Corollospora_[30; 41]. Reference sequences were retrieved from GenBank (Table 1). Sequences were aligned using MUSCLE (default conditions for gap openings and gap extension penalties), implemented in MEGA X (Molecular Evolutionary Genetics Analysis), visually inspected, and manually trimmed to delimit and discard ambiguously aligned \begin{table}
\begin{tabular}{c c c c} \hline \hline \multicolumn{3}{c}{**Forward and Reverse Primers**} & **Thermocycler Conditions** & **References** \\ \hline
**ITS** & ITS1-ITS4 & 95 \({}^{\circ}\)C: 5 min, (95 \({}^{\circ}\)C: 40 s, 55 \({}^{\circ}\)C: 50 s, 72 \({}^{\circ}\)C: 50 s) \(\times\) 35 & [35] \\  & & cycles; 72 \({}^{\circ}\)C: 8 min; 4 \({}^{\circ}\)C: \(\infty\) & [36] \\ \hline
**LSU** & LROR-LR7 & 95 \({}^{\circ}\)C: 5 min, (95 \({}^{\circ}\)C: 1 min, 50 \({}^{\circ}\)C: 1 min, 72 \({}^{\circ}\)C: 2 min) & [36] \\  & & \(\times\) 35 cycles; 72 \({}^{\circ}\)C: 10 min; 4 \({}^{\circ}\)C: \(\infty\) & [35] \\ \hline
**SSU** & NS1-NS4 & 95 \({}^{\circ}\)C: 5 min, (95 \({}^{\circ}\)C: 1 min, 50 \({}^{\circ}\)C: 1 min, 72 \({}^{\circ}\)C: 2 min) & [35] \\  & & \(\times\) 35 cycles; 72 \({}^{\circ}\)C: 10 min; 4 \({}^{\circ}\)C: \(\infty\) & [37] \\ \hline
**TEF-1\(\alpha\)** & EF-dF/EF-2218R & 95 \({}^{\circ}\)C: 5 min, (95 \({}^{\circ}\)C: 1 min, 50 \({}^{\circ}\)C: 1 min; 72 \({}^{\circ}\)C: 2 min) & [37] \\  & & \(\times\) 40 cycles, 72 \({}^{\circ}\)C: 10 min; 4 \({}^{\circ}\)C: \(\infty\) & [38] \\ \hline
**gTUB** & Bt2a-Bt2b & 94 \({}^{\circ}\)C: 4 min, (94 \({}^{\circ}\)C: 35 sec, 58 \({}^{\circ}\)C: 35 s, 72 \({}^{\circ}\)C: 50 s) \(\times\) & [38] \\  & & 35 cycles; 72 \({}^{\circ}\)C: 5 min; 4 \({}^{\circ}\)C: \(\infty\) & [39] \\ \hline
**RPB1** & RPB1 Af-RPB1 Cr & 96 \({}^{\circ}\)C: 5 min, (94 \({}^{\circ}\)C: 30 s, 52 \({}^{\circ}\)C: 30 s, 72 \({}^{\circ}\)C: 1 min) \(\times\) & [40] \\  & & 40 cycles; 72 \({}^{\circ}\)C: 8 min; 4 \({}^{\circ}\)C: \(\infty\) & [40] \\ \hline
**RPB2** & fRPB2-SF/fPB2-7 cR & 94 \({}^{\circ}\)C: 3 min, (94 \({}^{\circ}\)C: 30 s; 55 \({}^{\circ}\)C: 30 s; 72 \({}^{\circ}\)C: 1 min) \(\times\) & [40] \\  & & 40 cycles, 72 \({}^{\circ}\)C: 10 min; 4 \({}^{\circ}\)C: \(\infty\) & [40] \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Genetic markers, primers, and thermocycler conditions were used in this study. regions. Since no incongruence was observed among single-loci phylogenetic trees, alignments were concatenated into a single data matrix with SequenceMatrix [42]. The best evolutionary model under the Akaike Information Criterion (AIC) was determined with jModelTest 2 [43]. Phylogenetic inference was estimated using Maximum Likelihood (ML) and Bayesian Inference (BI) criteria. The ML analysis was generated using RAxML v. 8.1.2 [44] under GTR + I + G evolutionary model and 1000 bootstrap replicates. Support values from bootstrapping runs (MLB) were mapped on the globally best tree using the \"-f a\" option of RAxML and \"-x 12345\" as a random seed to invoke the novel rapid bootstrapping algorithm. BI was performed with MrBayes 3.2.2 [45] with the same substitution model (GTR + I + G). The alignment was run for 10 million generations with two independent runs each containing four Markov Chains Monte Carlo (MCMC) and sampling every 100 iterations. The first 25% of generated trees were discarded as \"burn-in\". A consensus tree was generated using the \"sumt\" function of MrBayes and Bayesian posterior probabilities (BPP) were calculated. Consensus trees were visualized in FigTree v. 1.4.2 ([[http://tree.bio.ed.ac.uk/software/figtree](http://tree.bio.ed.ac.uk/software/figtree)]([http://tree.bio.ed.ac.uk/software/figtree](http://tree.bio.ed.ac.uk/software/figtree)) accessed on 12 May 2021).