# Comparative Study of Ozonated Olive Oil and Extra Virgin Olive Oil Effects on Oral Hygiene
## 4 Discussion
Taking into account what has been described ozonated oil can represent and guarantee a determining factor in the treatment of gingivitis and oral lesions [22]. Studies have shown that after treatment, plaque index and bleeding index differ significantly from the indices of placebo-treated subjects in any comparison [28]. The ozonated oil may play a key role in the treatment of gingivitis and oral lesions, and there is also some preliminary evidence to suggest that it reduces the levels of severalcompounds associated with halitosis [26]. The studies carried out in the field of dentistry have led to considerable progress in the field of prevention and hygiene, with positive results, and therefore the use of ozonated oil guarantees this turning point in dental treatments [25; 26]. The study showed that ozonated olive oil, due to its organoleptic properties, the absence of toxicity and side effects, and its biocompatibility, represents a choice in the treatment of various oral diseases. Ozone has the power to kill bacteria, viruses, and fungi as well as other pathologies [27; 28]. Studies show that it provides soothing treatment for fungal infections, dermatitis, infection sites, periodontal pockets, and more. [PERSON] et al demonstrated that ozonated water strongly inhibits plaque accumulation in vitro, with high efficacy against Gram-positive and negative oral microorganisms [29]. Ozonated olive oil offers a quick and easy way to apply ozone directly where it is needed and facilitates healing. People using ozonated olive oil for teeth have noted success against tooth decay, gingivitis, and chapped lips [30; 31]. The release of ozone can be achieved in several ways because it becomes unstable when dissolved in water, it decomposes quickly through a complex series of chain reactions, so it cannot be stored [32]. Olive oil reacts with ozone thus forming long complex molecules [27]. Several studies confirm the fact that the use of ozonated olive oil gel has a longer duration of action in the oral cavity with beneficial effects in the treatment of oral diseases [28]. Ozone appears to strongly inhibit the formation of dental plaque and reduce the number of pathogens [16]. The oxidative power of ozone is 1.5 times that of chloride when used as an antimicrobial agent [6]. This oxidation effect gives ozone its bactericidal, virucidal, and fungicidal activity [12]. According to microbiological studies, ozone is able to kill Gram-positive and Gram-negative bacteria, including _Pseudomonas aeruginosa_ and _Escherichia coli_[6]. This antimicrobial capacity is the result of the effects of ozone on cells such as damage to the cytoplasmic membrane due to ozonolysis of double bonds and induction of cytoplasmic change contents. This action does not seem to harm the human body cells; the reason attributed to this is the antioxidant capacity of mammalian cells [18]. The therapeutic action of ozonated olive oil is its effectiveness in reducing periodontal indices. Subjects using ozonated olive oil for teeth have noted good responses in diminishing the rate of tooth decay, gingivitis, and chapped lips and ozonated olive oil could be considered an alternative antibacterial agent [28; 29]. There are also studies that have shown that ozonated olive oil promotes the healing of grafts with a very good prognosis [30]. A particularly important aspect of dentistry is the prevention of various diseases. There are studies that indicate that ozonated olive oil has the effect of improving the results of scaling and root planing (SRP) in the treatment of periodontal diseases [31; 32]. Compliance with tooth brushing procedures and the use of mouthwash with ozonated olive oil could be an additional preventive tools for the oral hygiene procedure at home. Further research is needed to evaluate the effectiveness of ozonated olive oil mouthwashes in patients at high risk of caries and subjects requiring orthodontic therapy [33; 34; 35]. One of the study's limitations is the small number of groups of study.