## 2 Materials and Methods  
In this study, we used forty-eight Wister rats, adults, with a similar weight, between 300-440 g, which were kept in the same conditions of light and humidity. Water and standard laboratory food for rats were freely provided to the animals, room temperature and alternating 12 h cycles of light and dark. At the end of our study the rats used were euthanized. The study received the Ethics opinion of \"[PERSON]\" University of Medicine and Pharmacy of Iasi. The tissue fragments taken were processed by the usual paraffin-embedded histopathological technique and stained with hematoxylin-eosin. We implanted with one microtextured breast implant, according to an approved institutional animal care protocol. The implants were each 2 ccs (2 cm diameter). Before surgical procedures, the rats were anesthetized with intramuscular administration of Ketamine 50 mg/mL, 0.3 mL/kg and Xylazine 2% 0.2 mL/kg. After the animals had been shaved and prepared prior to surgery, the skin of each rat was washed with 4 % Chlorhexiidine surgical scrub and their skin was disinfected with Betadine solution that contained 10% poyidone-iodine, according to the instructions for performing rodens surgery new drape and a new sterile gloves was used for each animal The animal was positioned in supine position that does not influence the surgical technique and the resultseither. The surgical procedure was performed in an animal operating theater following aseptic rules. Talc-free gloves were used at all times during the procedure. Implant pockets were developed throw an abdominal paramedian incision next to the mammary gland, in a retroglandular pocket with atraumatic dissection. Under direct vision, particular attention was paid to hemostasis, avoiding blunt instrumentation; there was no obvious bleeding. A new pair of talc-free gloves were worn when inserting the implants. 8 rats received an untreated implant (control), 17 rats an implant impregnated with rifampicin solution, 12 rats had implant combined with intramuscular dexamethasone injection for ten days and 11 rats had silicone implant associated with autologous centrifuged fat introduced in the implant pocket. The skin incisions were closed using 4-0 nylon sutures. Rats were sacrificed at eight weeks. Prior to sacrifice, each animal was anesthetized, and a 5-mm incision was made directly over the previous incision, through the skin, we identify the capsular tissue surrounding the silicone implant and we removed it after dissection through the skin incision. Capsule samples were immersed in 10% formalin and were submitted to histological evaluations. **Histological Assessment:** Capsule specimens were fixed with 10% buffered formalin and after 24 h were embedded in paraffin. The transversal sections were made in order to evaluate the capsular architecture. Afterwards were performed the hematoxylin and eosin staining and histological assessment for tissue inflammation and capsular thickness. Lymphocytes, granulocytes, macrophages, eosinophils, and mastocytes were the types of inflammatory cells evaluated in the capsule. The giant cellular reaction and the signs of acute and chronic inflammation were quantitated. Inflammatory infiltrate was categorized as mild, moderate, or severe, according to the intensity. **Statistical Analysis:** Statistical analysis was performed using the statistical software package IBM SPSS Statistics Version 20.0 (International Business Machines Corp., Armonk, New York, USA). The confidence interval (CI) was invariably calculated using the confidence interval analysis (CIA) software (3). Prior to the statistical analysis, the presumption of normality was performed using the Shapiro-Wilk test. Descriptive data were expressed as mean \(\pm\) standard deviation (SD), median with interquartile range (IQR), or relative frequency with 95% CI.