## 3 Results
### Metabolite Variance across Multiwell Fluidic System to Ascertain Sites of Generated Cell Byproducts
\begin{table}
\begin{tabular}{l l l} \hline \hline
**APAP-GSH** & Highest MR @ 24 h & Most well–well \(\Delta\) is 0.18 uM occurs at 24 h in midstream well 6 (Figure 9(a1)). \\ \hline Mean MR @ 24 h & Uniform well–well \(\Delta\) is measured in wells 4, 7, 8, and 9 (0.05 uM) (Figure 9(a1)). \\ \hline Negligeable MR @ 24 h & No well–well \(\Delta\) found in downstream wells 10 and 11 (0.0 uM) (Figure 9(a1)). \\ \hline MR Compilations @ 24, 48, 72 h & \(\blacksquare\) & 24 h mean (0.06 uM) and the UCL (0.24 uM) are highest when compared alongside GSH-48 h and GSH-72 h; Figure 9(a1–a3). \\  & \(\blacksquare\) & 48 h mean is least (0.042 uM) with UCL having a midrange distinction (0.125 uM); Figure 9(a2). \\  & \(\blacksquare\) & 72 h mean is midrange (0.051 uM) with UCL having the lowest distinction (0.11 uM) to divulge least variation \(\Delta\)’s across device culture wells; Figure 9(a3). \\ \hline In Brief (Figure 9(a1–a3)): & 3\(\sigma\) UCL is maximum at 24 h and decreases over time to indicate that cell generated APAP-GSH has the highest variance during early APAP exposure periods in upstream sites (wells 3–5; 0.05 uM; Figure 9(a1)) and conditions midstream culture wells with cell byproducts (well 6; 0.18 uM). \\ \hline
**APAP-Sulfate** & Highest MR @ 72 h & Most well–well as 12.79 uM and 11.11 uM occurring at 72 h in downstream wells 9 and 10 (Figure 9(b3)). \\ \hline Mean MR @ 72 h & Uniform well–well \(\Delta\)s are present in upstream wells 4–8 (0.84–4.9 uM) and downstream well 11 (0.9 uM) (Figure 9(b3)). \\ \hline Negligeable MR @ 24 h & Limited well–well \(\Delta\) found in upstream wells 4 and 5 (–0.0 uM) (Figure 9(b1)). \\ \hline MR Compilations @ 24, 48, 72 h & \(\blacksquare\) & 24 h data mean is lowest (2.0 uM) with UCL having the lowest distinction (7.0 uM) to divulge least variation \(\Delta\)’s across device culture wells; Figure 9(b1). \(\blacksquare\)8 h data mean is midrange (2.8 uM) with UCL also having a midrange distinction (9.0 uM); Figure 9(b2). \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Summary of APAP-Metabolite variance from HepaRGs—generation and dissemination. Cell Function Variance across Multiwell Fluidic System to Assess Bio-Activity Trends Affiliated with Direct Drug Exposure and Predictive Recognition for Indirect Cell-Byproduct Stimuli (Dual Assessment Screens)  
Conventional cell function assays LDH, CYP3A4, and GSH are re-purposed as ulfudic device biomarkers and utilized as cell function indicators (sensors) across the platform's changing well-well environments, i.e., monitoring worksites across an assembly line. Assays are used to track mutable cell-health kinetics, throughout uchannel wells 3-11, corresponding to real-time ramps in drug concentrations, accruing exposure times, and for gaging the incidence of cell-generated byproducts. To initiate surveys, an exposure compound is dosed into the upstream source well 1, daily for 7 days. Compounds in this study are DMSO control vehicle (0.1%), ASA (25 \(\upmu\)M; non-DILI drug), and NEF (16 \(\upmu\)M; DILI drug). With flow, compounds auto-disperse along the uchannel, catalyzing adaptive bioactivity amid aligned device culture site locations, i.e., wells 3-11. To reveal if gradients of drug, time, or cellular byproducts have influence on cell functions, we assessed both system-trends and adjacent-well responses, i.e., six sigma control charts, from two hepatic sources: human HepaRG cells and rat hepatocytes.