## 3 Results and Discussion
### Determination of judgment Values for Standard and Test Samples  
Because a minimum sample of 24 single grains is required for 95% reliability, 200 g of the collected sample was evenly distributed with a grain spreader, and 24 single grains were ultimately sampled [18]. After DNA extraction, multiplex PCR analysis was performed on the samples using the 11 InDel markers that were assigned unique scores. Then, the scores given to the amplified markers were calculated to confirm the judgment values. First, we confirmed, through analysis, whether the 16 standard samples were clearly identified. Among the 16 varieties (15 domestic and 1 imported), 14 varieties (13 domestic and 1 imported) could be discriminated (Table 2). The two domestic varieties that could not be discriminated were Seonyu and Hwangkeumol, which shared an identical judgment value (655). \begin{table}
\begin{tabular}{c c c c c c} \hline \hline \multirow{2}{*}{**Set**} & \multirow{2}{*}{**Marker**} & \multicolumn{2}{c}{**Final Primer**} & \multirow{2}{*}{**Forward Sequence**} & \multirow{2}{*}{**Reverse Sequence**} & \multirow{2}{*}{**Size (bp)**} \\ \cline{3-3} \cline{5-6}  & & & & & \\ \hline A & 1 & 1.05 & CCAATTCGATCTTTACCAATTCA & TACCATATTAATCAAAACATTTTTCTAAA & 102 \\ \hline \multirow{3}{*}{B} & 3 & 0.09 & TTGGACCTGAGCCGTGGAGC & CACCCAAAATGCGTCATTAGCC & 473 \\ \cline{2-6}  & 4 & 0.35 & CAGAGCTTCAGTTCTTGCACTCAG & TCAAGAACAACAACATAGGAAGATG & 138 \\ \cline{2-6}  & 11 & 1.05 & CGAAAAATTTTGAAAATACTGTAGAGGA & GCAGCTTCTTGCAAAAATG & 112 \\ \hline \multirow{3}{*}{C} & 2 & 0.18 & TGAGTGGTGTGTGTGAATAAGTCTT & TGATGGGTTGAGGCTCTAT & 238 \\ \cline{2-6}  & 5 & 1.05 & CACCCACTCGTTTTACTCG & GCGGTTTGACTGGATTGG & 107 \\ \hline \multirow{3}{*}{D} & 6 & 1.05 & TGATTTGGACAACATATATACGTG & GCGACATAAACACATGGAAC & 459 \\ \cline{2-6}  & 7 & 0.35 & CCTTGGTCTCCACTGCG & TTCGTATGGGGGTTCAAAA & 246 \\ \cline{2-6}  & 8 & 0.35 & GGGCATGTCGTAAGCTTGTC & CCACTACCCCCAAAACGTAT & 112 \\ \hline E & 9 & 0.18 & TTCTTTTGAGTATTCCCTTCTG & TAGGTGCCTTACGAAAGTATTATTATA & 324 \\ \hline F & 10 & 0.35 & GCGAATCCAAGACCTAAGTCAG & AAACCACTGGGCCCTTTA & 872 \\ \hline \hline \end{tabular}
\end{table} 
Table 1: A list of the eleven allele-specific PCR markers used for the origin discrimination of soybean in this study. Figure 3: Simultaneous detection performance and specificity of multiplex PCR based on 11 InDel markers for soybean origin identification by **(A)** agarose gel electrophoresis and **(B)** capillary electrophoresis. Lane M: **(A)** 100 bp DNA ladder (ST-M100, TNT Research, Korea), **(B)** 15 bp to 3 kb DNA ladder (QIAxcel DNA Fast Analysis kit, QIAGEN, Hilden, Germany); lane A: marker 1 (102 bp); lane B: marker 3 (473 bp), marker 4 (138 bp), marker 11 (112 bp); lane C: marker 2 (238 bp), marker 5 (107 bp); lane D: marker 6 (459 bp), marker 7 (246 bp), marker 8 (112 bp); lane E: marker 9 (324 bp); lane F: marker 10 (872 bp). Figure 2: Polymerase chain reaction amplification of 11 markers for soybean origin discrimination. **(A)** Marker 1 (102 bp), **(B)** marker 2 (238 bp), **(C)** marker 3 (473 bp), **(D)** marker 4 (138 bp), **(E)** marker 5 (107 bp), **(F)** marker 6 (459 bp), **(G)** marker 7 (246 bp), **(H)** marker 8 (112 bp), **(I)** marker 9 (324 bp), **(J)** marker 10 (872 bp), **(K)** marker 11 (112 bp). Second, to set the judgment value for a variety of soybean samples, 1096 soybean samples were collected to include the domestic soybeans cultivated in South Korea, those imported through the port of entry, and the foreign soybeans currently being distributed. As a result, domestic and imported soybean varieties could be classified based on 53 and 70 judgment values, respectively (Tables 3 and 4). These varieties had four overlapping judgment values (671; 1183; 1215; and 1695) that prevented origin discrimination across the corresponding domestic and imported soybeans.