## 3 Results
### Bacteriophage Lytic Performance  
For the phage lysis ability test in real time, bacteriophage performance against bacteria was tested in the presence of selected extracts at different concentrations, assessed by the changes in optical density measurements when the samples were cultivated in the bioreactors with mixing (150 rpm) (Figure 5). The extract concentrations were chosen based on the results from synogram experiment (combinations in which the above-described phenomena were present, along with maximal treatment doses as the controls). Experiment time was also shortened (16 h), as the synogram experiment (>18 h) revealed unactive cells. For most of the tested samples, the darkening of the sample was present due to the dark colour of the concentrated extract. This was taken into account when analysing the data: the extract optical density background was removed from the curves for a clear interpretation of the results. The tested extracts influenced bacterial cells and phage activity in a varied manner; however, some dependencies were noted (Figure 5). In general, high concentrations (25%) of the tested extracts resulted in a stimulating effect on bacterial cells, often cancelling the lytic effect of the phages. The combination of _P. syringae_, phage phie and EP at concentrations of 25% and 6.25%, resulted in growth curves similar to the control bacterial curve: no phage lysis was observed. However, the treatment combination when the EP concentration was 0.049% led to the phie lysis curve to be the same as the control lysis curve (only phage and bacteria) (Figure 5A). Mixtures of the MS2 phage and 25% EP extract initially resulted in a similar, though slightly worse, lysis of _E. coli_ DSM 5695 at 2 h to 4 h incubation. After this time, there was a clear rebound of the curve, suggesting increased bacterial proliferation. The reduction in the EP concentration to 6.25% also led to the bacterial lysis curve being weakened, but similar to the control lysis curve; however, the lysis started quicker (after 1 h vs. 2 h for the control), and later on, it also ended quicker (after 1.5 h vs. 3 h for the control). The EP concentration of 0.049% resulted in almost identical lysis curve as in the control lysis sample, except the low extract concentration also quickened the lysis visibly (Figure 5A'). When T4 phage was present, the high concentration of the EP extract (25%) eliminated the phage lytic effect after the first 2 h of incubation, simultaneously increasing the proliferation of _E. coli_ DSM 613 cells. After 2 h, the sample optical density (proliferated bacterial biomass) started to drop very slowly. The EP concentration of 6.25% resulted in a very similar growth curve as the 25% concentration, until 6 h of incubation. After that time, a visible drop in the OD was observed, probably caused by the T4 phage activity. Finally, when 0.049% of the EP extract was added to the bacteria-phage sample, after the first 10 h of incubation, the curve was fairly similar to the control bacterial growth curve, meaning that the bacterial proliferation was only slightly altered. Then, after 10 h, a visible drop in the OD was observed, as it was in the EP 6.25% sample, suggesting the occurrence of phage activity (Figure 5A\"). When the RG extract was used (25%) along with the phie phage, a very high proliferation rate of _P. syringae_ was observed after 3.5 h of incubation. After 10 h, the growth curve stabilised, suggesting that the bacterial stationary phase was reached (Figure 5B). The reduction in the RG concentration to 0.78% led to the observable lysis of the phie phage. However, the lysis was delayed, since it started after 5 h of incubation (control lysis started after 2 h) and also ended later in time (after 8 h, compared to the control that ended after 3.5 h), reaching the final OD values that were almost the same as that of the lysis control (Figure 5B(II)). When the RG extract (25% and 3.125%) was mixed with the MS2 phage and added to the _E.