## 2 Results and Discussion
### Sensory Analysis
\begin{table}
\begin{tabular}{c c c c c} \hline \hline
**No** & **Compound** & **Standard Curve** & \(\mathbf{r^{2}}\) & **Validation Range (\(\mathbf{\mu}\)g kg\({}^{-1}\))** \\ \hline
**1** & Methanethiol & y = 0.065x \(-\) 0.0037 & 0.986 & 1–10 \\
**2** & Dimethyl trisulfide & y = 1.7x + 0.0373 & 0.973 & 500–5000 \\
**3** & 2-Methylbutanal & y = 4.53x \(-\) 0.00591 & 0.981 & 50–500 \\
**4** & 3-Methylbutanal & y = 0.84x + 0.109 & 0.971 & 10–5000 \\
**5** & Pentanal & y = 1.27x + 0.054 & 0.982 & 1–20 \\
**6** & Isopropyl alcohol & y = 2.13x + 0.0016 & 0.993 & 1–10 \\
**7** & 1-Propanol & y = 3.39x \(-\) 0.0303 & 0.942 & 50–500 \\
**8** & 1-Butanol & y = 1.16x \(-\) 0.0239 & 0.996 & 1–10 \\
**9** & Hexanal & y = 0.70x \(-\) 0.0531 & 0.971 & 10–100 \\
**10** & Dimethyl disulfide & y = 4.53x \(-\) 0.591 & 0.968 & 10–2000 \\
**11** & 2-Methylpropanol & y = 0.99x \(-\) 0.0477 & 0.987 & 1–500 \\
**12** & (\(E\))-2-Methyl-2-butenal & y = 1.51x \(+\) 0.019 & 0.979 & 10–500 \\
**13** & Limonene & y = 3.40x \(-\) 0.041 & 0.988 & 1–50 \\
**14** & Heptenal & y = 1.56x \(-\) 0.0193 & 0.984 & 1–500 \\
**15** & 2-Butenal & y = 4.73x \(-\) 0.025 & 0.983 & 5–200 \\
**16** & 2-Methylbutanol & y = 0.91x \(+\) 0.031 & 0.986 & 500–5000 \\
**17** & 3-Methylbutanol & y = 1.05x \(+\) 0.076 & 0.985 & 5–50 \\
**18** & 2-Pentylfuran & y = 0.80x \(+\) 0.0492 & 0.971 & 5–100 \\
**19** & 3-Octanone & y = 0.38x \(+\) 0.0852 & 0.997 & 500–10000 \\
**20** & 1-Pentanol & y = 0.88x \(-\) 0.017 & 0.992 & 1–10 \\
**21** & Bis(methylthio) mathane & y = 0.9x \(+\) 0.145 & 0.977 & 2–20 \\
**22** & 2-Octanone & y = 0.26 \(-\) 0.0138 & 0.993 & 2–20 \\
**23** & 4-Isopropyltoluene & y = 0.1277x \(+\) 0.00985 & 0.987 & 0.1–5 \\
**24** & Octanal & y = 1.654x \(-\) 0.0235 & 0.973 & 20–1000 \\
**25** & 1-Octen-3-one & y = 1.977x \(+\) 0.0713 & 0.986 & 50–2000 \\
**26** & Isobutyl hexanoate & y = 1.488x \(-\) 0.0790 & 0.982 & 0.1–5 \\
**27** & Nonanal & y = 1.61x \(-\) 0.0233 & 0.985 & 10–100 \\
**28** & Heptenoic acid ethyl ester & y = 2.87x \(-\) 0.0188 & 0.976 & 1–20 \\
**29** & 1-Hexanol & y = 1.70 \(-\) 0.0918 & 0.992 & 1–10 \\
**30** & 3-Octanol & y = 0.93x \(+\) 0.029 & 0.969 & 200–5000 \\
**31** & Octanoic acid ethyl ester & y = 1.07 \(+\) 0.068 & 0.983 & 5–50 \\
**32** & (E)-2-Octenal & y = 1.50x \(-\) 0.0218 & 0.979 & 1–10 \\
**33** & (E)-2-Nonneal & y = 2.59x \(-\) 0.0376 & 0.986 & 2–50 \\
**34** & P-cresyl methyl ether & y = 0.79 \(-\) 0.0034 & 0.967 & 20–200 \\
**35** & 1-Heptonal & y = 1.49x \(-\) 0.00208 & 0.983 & 1–10 \\
**36** & 1-Octen-3-ol & y = 2.13x \(-\) 0.0289 & 0.995 & 200–10000 \\
**37** & 3-(Methylthio)propanal & y = 2.48x \(+\) 0.0102 & 0.992 & 0.5–5 \\
**38** & 1-Octanol & y = 3.51x \(-\) 0.0472 & 0.992 & 1–10 \\
**39** & Dimethyl sulfoxide & y = 0.30x \(-\) 0.0446 & 0.989 & 1–10 \\
**40** & Benzaldehyde & y = 0.40x \(-\) 0.0595 & 0.976 & 0.5–10 \\
**41** & 2-Methylbutanoic acid & y = 0.78x \(-\) 0.0421 & 0.971 & 2–20 \\
**42** & 3-Methylbutanoic acid & y = 0.89x \(-\) 0.0086 & 0.975 & 1–10 \\
**43** & 2-Acetylthiazoe & y = 1.25x \(+\) 0.41 & 0.965 & 1–10 \\
**44** & Benzeneacetaldehyde & y = 1.77x \(-\) 0.0142 & 0.982 & 20–500 \\
**45** & Dipropyl trisulfide & y = 2.40x \(-\) 0.0320 & 0.982 & 2–10 \\
**46** & 3-(Methylthio)propanol & y = 2.072x \(-\) 0.0282 & 0.979 & 2–20 \\
**47** & Benzyl alcohol & y = 2.47x \(+\) 0.0079 & 0.987 & 0.05–1 \\
**48** & Dimethyl sulfone & y = 1.97x \(+\) 0.031 & 0.981 & 1–10 \\
**49** & Phenylethyl alcohol & y = 4.95x \(-\) 0.0356 & 0.973 & 2–500 \\
**50** & \(\Gamma\)-Nonalactone & y = 0.78x \(+\) 0.0512 & 0.988 & 2–20 \\
**51** & [Bis(2-methyl-3-furyl) disulfide] & y = 2.97x \(+\) 0.0053 & 0.976 & 0.2–2 \\ \hline \hline \end{tabular}
\end{table} 
Table 3: Standard curves, validation range and correlation coefficients of standards (\(\mathbf{r^{2}}\)) for the volatile compounds in truffle samples. PLSR was applied to certify the correlation between the GC-O data and flavor notes by the panelists.