## 3 Results and Discussion
\\ \hline
49 & 11.92 & 288, 282 & 3,4-didehydrothomson-2 & & C2H\({}_{4}\)NaSO\({}_{0}\)+ & - & 32L358 & - & 165.6288, 117.039 & c \\
50 & 12.00 & 234, 277 & 3,4-didehydrothomson-2 & & C2H\({}_{4}\)NaSO\({}_{0}\)+ & - & 32L368 & - & 165.6288, 117.039 & n, g-g g g \\  & & & 3,4-didehydrothomson-2 & & & C2H\({}_{4}\)NaSO\({}_{0}\)+ & - & 32L355 & 1.12 & 165.6288, 117.039 & n \\
51 & 12.15 & 228, 324 & derivative & & C2H\({}_{4}\)NaSO\({}_{0}\)+ & - & 32L355 & 1.12 & 165.6288, 117.039 & n \\
52 & 12.85 & 259 & Unknown & & C2H\({}_{4}\)NaSO\({}_{0}\)+ & - & 32L358 & - & 115.6288, 118.0355 & n \\
53 & 13.80 & 285 & & - & - & 34L342 & - & 165.0328, 117.039 & n \\  & & & derivative & & & & 165.0328, 117.039 & n \\
54 & 14.75 & 220, 260 & Tokita-old & C4H\({}_{0}\)+ & 137.0397 & 137.0601 & 2.84 & 107.0398, 120.035 & n \\  & & & & & & 107.0398 & 107.0601 & 2.84 & 107.0398, 120.035 & n \\
55 & 14.74 & 221, 223, 260 & Tokita-old & C4H\({}_{0}\)+ & 137.0397 & 137.0601 & 2.84 & 107.0398, 120.035, 120.035, & c, n+g g g \\
56 & 16.36 & 283 & Preon & C4H\({}_{0}\)O\({}_{+}\)+ & 299.1123 & 294.1233 & 6.83 & 107.0398, 120.035, & c, n+g g \\
57 & 17.08 & 221, 286 & Tokita-old & C4H\({}_{0}\)O\({}_{+}\) & 137.0397 & 137.0601 & 5.21 & 107.0398, 120.035, & n \\
58 & 15.27 & 285 & Preonon & C4H\({}_{0}\)O\({}_{+}\)+ & 299.1123 & 294.123 & 6.83 & 107.0398, 120.035, & n \\
59 & 18.70 & 290 & Tokita-old derivative & & & 352.2344 & - & 137.0398, 120.035, & n \\
60 & 19.50 & 283 & Launch/discontinuing & C4H\({}_{0}\)NaSO\({}_{0}\)+ & 271.2740 & 274.2758 & 5.5 & 264.332, 262.034, 262.034, & n \\
61 & 19.70 & 292 & Tokita-old derivative & & & 202.2487 & - & 137.0398, 121.049 & c, n+g g \\
62 & 19.90 & 276 & dide  
Figure 2: Proposed fragmentation pattern and MS2 scan of (**a**) salsoline (compounds **4**, **13**, **15**, **17**, **18**, **19**, **21**, **26**, and **42**), (**b**) N-methylphenylalanine (compound **8**), (**c**) 3-Amino-2-naphthoic acid (compounds **22**, **23**, **29**, and **33**), and (**d**) 1,2-Ethanediol, 1-(3-ethehenylphenyl) (compounds **24** and **28**). Peak **32** occurred exclusively in the callus tissue, and it was assigned as 3,4,5-triethoxyp henethylamine since pseudomolecular ion at \(m/z\): 254.1764 yielded one main fragment at \(m/z\): 138.0918 ([M+H-2C\({}_{2}\)H\({}_{6}\)O-C\({}_{2}\)H\({}_{6}\)]\({}^{+}\)). This metabolite is commonly known as trisescaline, and it is structurally related to mescaline (an alkaloid that is present in cacti species such as peyote and San Pedro cactus, two species with recognized pharmacological and hallucinogenic properties [45; 46]). On the other hand, peak **36** was assigned as maclurin since the pseudomolecular ion at \(m/z\): 263.0542 produced fragments at \(m/z\): 121.0647 and 107.0491. The signal at \(m/z\): 121.0647 was generated by the fragmentation of the phenyl group and the elimination of two molecules of water; meanwhile, the fragment at \(m/z\): 107.0491 was generated by the subsequent elimination of one methyl group from the aromatic moiety of the fragment at \(m/z\): 121.0647. This compound was only detected in the aerial section of the in vitro plants, and it belongs to a class of metabolites known as benzophenones, a class of metabolites with applications in the cosmetic industry. Compound **37** was only detected in the greenhouse plants. This compound was assigned as 3,4-dihydro-8-hydroxy-6,7-dimethoxy-1-methylisoquinoline since the pseudomolecular ion at \(m/z\): 222.1124 yielded fragments at \(m/z\): 162.0917 and 148.0761. The fragment at \(m/z\): 162.0917 was generated by the loss of two methoxy groups, and the fragment at \(m/z\): 148.0761 was generated by the subsequent elimination of one methyl group. Similarly, compounds **38**, **41**, **47**, **49**, **50**, **51**, and **53** were also detected in the callus tissue, as was the case in the aerial section of the in vitro plants. In addition, this metabolite was detected in the whole plant that grew under greenhouse conditions.