Incrementally with the specific evolution of the times in most scientific fields, these materials--as our study follows--other new as well, with higher impact upon tissue regeneration, but which due to certain limitations by different socio-economic criteria and specific condition have not yet been studied by us, being mentioned only. Bioactive biocearamic (BA) materials used in endodontic treatment promote pulpal and periapical tissue healing and are easy to use. They are dental materials used extensively for vital pulp therapy, protecting scaffolds during regenerative endodontic procedures, apical barriers in teeth with necrotic pulps and open apices, perforation repairs as well as root canal filling, and root-end filling during surgical endodontics [6]. These materials can be placed in contact with both soft and hard vital tissues, influencing the final result of the treatment and, therefore, their biocompatibility and regenerative properties are of critical importance [7,8]. The biological properties of root canal sealers and the degree of cytotoxicity or stimulation they manifest on different cells, including fibroblasts, osteoblasts, and cementoblasts must be evaluated in vivo as well as in vitro. The main characteristic properties of MTA include superior sealing ability, biocompatibility,antimicrobial effect, radiopacity, dimensional stability, and tolerance to moisture over other dental materials such as IRM, amalgam, Ca(OH)\({}_{2}\), super EBA, or ZOE. Its composition was described as primarily a mixture of calcium silicates comprised of calcium oxide (CaO) and silicon dioxide (SiO\({}_{2}\)). MTA restoratives are typically tested with a mixture of tests from multiple standards. As the setting of MTA is dependent upon hydration, the results of various MTA restoratives and sealers are dependent upon the healing approach. Since its design, MTA has been tested under laboratory conditions, in animal studies and in clinical trials as well [9]. Another product that is not marketed as MTA but is chemically similar is the BioAggregate (BA-Innovative BioCeramix, Inc., Vancouver, BC, Canada), a biocompatible pure white powder composed of ceramic nanoparticle upon mixing DiaRoot with BioA. It differs from MTA, being aluminum-free and containing calcium phosphate monobasic and tantalum pentoxide. BA also includes hydroxyapatite and amorphous silicon oxide. Hydroxyapatite and amorphous silicon oxide are added by the manufacturer to reduce the levels of the calcium hydroxide, which does not contribute to the strength of the material. Polymeric calcium hydroxide root canal sealer named Sealapex, the original non-eugenol, calcium hydroxide polymeric root canal sealant produces rapid healing and hard tissue formation issue confirmed by some histopathological analysis studies that showed it was the best sealer that permitted the deposition of mineralized tissue at the apical level and also the only sealer that provided complete sealing with no inflammatory infiltrate occurring and no reabsorption of mineralize tissues [10]. Several studies in the literature report various results regarding the tested sealers, cell type, time of cell exposure to materials, extract dilutions, and specimen size [11]. However, there are few studies that evaluate the cytotoxic effect of root canal sealers in an in vivo condition and, also in a reproducible model that comes close to the real response to the area of exposure of the apical foramen diameter without using animal models [12]. In most of clinical situations, the endodontic treatment is followed by an unidental or bridge component prosthetic restoration. As a matter of theory issue, this stage framed of the treatment plan--in non-specific preprosthetic treatments part, is considered as a premise of restoring the prosthetic support that needs an increased resistance considering the height power of masticatory forces that require an optimally functionality in the stomatognathic system areal.