## 3 Chemical Composition of _Hyssopus officinalis_ L. and _Agastache foeniculum_ (Pursh) Kuntze EOs
### Chemical Composition of Agastache foneticulum (Pursh) Kuntze EO
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17 & NM & NM & \begin{tabular}{c} **Methyl chavicol**: 3.2; \\ **Mentbone**: 34.3; \\ **Isomentbone**: 14.4; \\ **Pulegone**: 9.1; \\ **Methyl eugenol**: 3.1 \\ \end{tabular}  & \begin{tabular}{c} **Methyl chavicol**: 3.2; \\ **Mentbone**: 34.3; \\ **Mentb**: 14.4; \\ **Pulegone**: 9.1; \\ **Methyl eugenol**: 3.1 \\ \end{tabular}  & \begin{tabular}{c} **Methyl chavicol**: 3.2; \\ **Mentbone**: 14.3; \\ **Pulegone**: 9.1; \\ **-Caryophyllene**: 3.1 \\ \end{tabular}  & \ \begin{tabular}{c} **Methyl chavicol**: 3.2; \\ **Mentbone**: 34.3; \\ **Mentb**: 14.4; \\ **-Caryophyllene**: 3.4 \\ \end{tabular}  & \begin{tabular}{c} **[42]** \\ **[42]** \\ **[42]** \\ \end{tabular}  \\ \hline
34 & 99.63 & \begin{tabular}{c} **Phenylpropanoids**: 69.79 \\ **Sesquietpene hydrocarbons**: 19.19 \\ **Monoterpene hydrocarbons**: 6.29 \\ **Oxygenated monoterepenes**: 0.46 \\ **Oxygenated sesquiterpenes**: 2.42 \\ **Other**: 1.85 \\ \end{tabular}  & \begin{tabular}{c} **Stragole:** 63.27; \\ **Caryophyllene**: 10.44; \\ **Limonene**: 6.29; \\ **-Caryophyllene**: 4.34; \\ **-Caryophyllene**: 22.39 \\ **-Caryophyllene**: 9.23 \\ **-Caryophyllene**: 31.58; \\ **-Caryophyllene**: 54.51 \\ **-Caryophyllene**: 5.03 \\ \end{tabular}  & \begin{tabular}{c} **Stragole:** 60.648, \\ **E02**: 30.16, EO3: 88.09; \\ **Limonene**: E01: 52.4, EO3: 8.01; \\ **-Caryophyllene**: 1.04, EO2: 14.22; \\ **-Caryophyllene**: 1.07; \\ **-Caryophyllene**: 1.08; \\ **-Caryophyllene**: 5.03 \\ \end{tabular}  & \begin{tabular}{c} **Stragole:** 60.648, \\ EO2: 30.16, EO3: 88.09; \\ **Limonene**: E01: 52.4, EO3: 8.01; \\ **-Caryophyll**: 1.04, EO2: 14.22; \\ **-Caryophyll eugenol**: EO1: 9.77; \\ **-Caryophyllene**: 1.39.6; \\ **-Caryophyllene**: 5.02, EO2: 2.44; \\ **-Caryophyllene**: 5.03 \\ \end{tabular}  & \ \begin{tabular}{c} **Stragole:** 60.648, \\ EO2: 30.16, EO3: 88.09; \\ **Limonene**: E01: 52.4, EO3: 8.01; \\ **-Caryophyll**: 1.04, EO2: 14.22; \\ **-Caryophyll eugenol**: EO1: 9.77; \\ **-Caryophyllene**: 1.39.6; \\ **-Caryophyllene**: 5.02, EO2: 2.44; \\ **-Caryophyllene**: 5.03 \\ \end{tabular}  &
\begin{tabular}{c} **Stragole:** 60.648, \\ EO2: 30.16, EO3: 88.09; \\ **Limonene**: E01: 52.4, EO3: 8.01; \\ **-Caryophyll**: 1.04, EO2: 14.22; \\ **-Caryophyll eugenol**: EO1: 9.77; \\ **-Caryophyllene**: 1.39.6; \\ **-Caryophyllene**: 1.520, EO2: 2.44; \\ **-Caryophyll**: 1.45, EO2: 3.13; \\ **-Caryophyll**: 1.02**: 2.019; \\ **-Caryophyll**: 1.02**: 2.02; 4.53 \\According to [PERSON] et al., 2021 [41], five distinct chemotypes of _A. fonciculum_ were identified based on EO chemical profiles: methyl chavicol (estragole), spathulenol/bornyl acetate, \(\delta\)-cadinene/\(\beta\)-cadinol, limonene and isomenthone. They also tested three samples of _H. officialis_ EO obtained from plants grown under similar conditions (full sun, clayey-loamy sand) and reported no significant differences in the composition of the compounds. [PERSON] et al., 2024 [17] showed a difference between two species of _A. fonciculum_ in which  
Figure 4: The mean values (%) of the most reported compound classes from _Agastache fonciculum_ EO. Figure 5: The chemical structures of the main compounds of _A. fonciculum_ EO (Created with BioRender.com; accessed on 2 April 2025). one had the main compound estragole and the other menthone and pulogone. [PERSON] et al., 2022 [43] demonstrated a significant difference between _H. officinalis_ EO obtained from different parts of the plant. EO obtained from flowers had the higher amount of estragole, at 88.09%, followed by limonene (8.01%). Meanwhile, EO obtained from leaves had a more balanced composition; the main compounds being estragole, chavicol, eugenol, and phenyl ethyl alcohol. Additionally, significant amounts of methyl eugenol were reported besides estragole in the EO extracted from the entire aerial parts. The major considerations related to the various chemotypes must be identified and standardized to ensure their efficacy and safety, thereby guaranteeing their safe use by consumers [29].