## 1 Introduction  
Polysaccharides of microbial origin are carbohydrate polymers with a high molecular weight (MW) produced by microorganisms such as bacteria, fungi, yeast, and microalgae [1]. Polysaccharides in the cell may be found as intracellular polysaccharides, which accumulate in the cytoplasm as energy and carbon reserves (e.g., glycogen). They may be found in the cell walls, helping microbial cells to maintain their structural integrity (e.g., chitin) or polysaccharides that are produced outside the cell (exogenous polysaccharides) that either remain as capsular polysaccharides attached to the cell surface (CPS) or as a slime which is figuratively attached to the cell (EPS) [1]. Exopolysaccharides (EPS) are produced by a variety of microorganisms, where non-structural storage carbohydrates are produced by a few plant species. Homopolysaccharides and heteropolysaccharides are two different types of EPS, in which heteropolysaccharides are made up of various types of sugar monomers arranged in oligosaccharide repeating units, and homopolysaccharides are made up of only one type of constituting monosaccharide [2]. Levan is a fructose homopolymer that is naturally produced by a variety of microbes. _Bacillus_ sp. are known as good producer of levan, including _Bacillus subtilis_, _Bacillus licheniformis_, _Lactobacillus reuteri_, BKAG21, _Bacillus amyloliqueefaciens_, and _Bacillus licheniformis_ BKAG21 [3]. Biofabrication of levan from _B. subtilis_ has been reported in many studies. [PERSON] et al. reported the production of the polymer levan from _B. subtilis_ NATTO [4]. [PERSON] et al. reported that two strains of _B. subtilis_ exhibited in vitro promising probiotic characteristics [5]. Moreover, [PERSON] et al. reported the antioxidant activities of a levan produced from a new isolated _B. subtilis_ AF17 [3]. Levanscurase is a fructosyltransferase enzyme that aids in the conversion of sucrose to glucose and fructose, resulting in -(2,6)-levan synthesis [4]. Levan is also known as -2,6-D-fructan because of its biodegradability, biocompatibility, and film-forming ability. Levan finds many uses in the medical, food, and cosme industries. In food manufacturing, levan is used as flavor- and color-stabilizing agent [3], while in the cosmetic industry it is used as a hair care and skin whitening agent. In addition, in the medical industry, it is used in wound and burn healing, an anti-inflammatory, an antimicrobial, an antioxidant, an anti-irritant, an anticancer agent, and as a blood plasma substitute [5]. The main function of intact skin is to maintain body homeostasis by acting as a mechanical barrier that prevents the entry of pathogens into the interior environment of the body and prevents the loss of electrolytes to the outer environment as well [6]. Skin damage due to burning, cutting, or trauma is a challenging problem in medicine, especially with the growing problem of antibiotic resistance. The disruption of skin integrity, particularly due to burns, damages the epidermal barrier and causes denaturation of structural proteins and lipids in the skin, which leaves the burned area susceptible to infection [7]. The use of topical agents, especially those with antimicrobial activity, has significantly decreased the mortality rate due to burn contamination. It is also a great advantage when the topical antimicrobial agent does not retard wound healing but accelerates the process of healing instead [8]. Furthermore, before employing levan in any application, it must first undergo characterization to establish its chemical and physical composition, its morphological, mechanical, and thermal characteristics, and its molecular weight and other chemical properties [9]. Several techniques, such as Fourier transform infrared (FT-IR), nuclear magnetic analysis (\({}^{1}\)H and \({}^{13}\)C NMR spectroscopy), and others, were used for the characterization of levan polymers [10]. In this study, _Bacillus subtilis_ MZ292983.1, a levan producer, was isolated from soil.