## 3 Results and Discussion
### Wilcoxon Matched Pairs Test of the NADES Samples  
Pairwise comparison of samples observed at the beginning of the experiment and after 3 and 6 months was performed using the Wilcoxon Matched Pairs Test to test whether the mean values of the samples differed over time (Table 5). The significant composition change between 3 and 6 months was noticeable for the control, Bet/Suc/Pro/W, Bet/Gly/Suc/W, Pro/Gly/Sor/W, and Gly/Glu/Sor/W samples. The Wilcoxon test implied the stability of the Gly/Glu, Bet/Sor/W and Bet/EG samples for 6 months. \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Start of the** & & **Monitoring after 3** & **Monitoring after 6** \\
**Monitoring** & & **Months** & **Months** \\ \hline Cont0 & & Cont3 & & Cont6 \\ \hline \multirow{6}{*}{Gly/Glu0} & \(N_{\text{valid}}=54\) & & \(N_{\text{valid}}=62\) & \\  & \(T=476.5\) & & \(T=500.5\) & \\  & \(Z=2.290\) & & \(Z=3.337\) & \\  & \(p=0.022\) & & \(p=0.001\) & \\ \hline Gly/Glu0 & & Gly/Glu3 & & Gly/Glu6 \\ \hline \multirow{6}{*}{Gly/Suc/W0} & \(N_{\text{valid}}=48\) & & \(N_{\text{valid}}=51\) & \\  & \(T=472.5\) & & \(T=423.5\) & \\  & \(Z=1.185\) & & \(Z=2.245\) & \\  & \(p=0.236\) & & \(p=0.025\) & \\ \hline Bet/Suc/Pro/W0 & & & Bet/Suc/Pro/W3 & \\ \hline \multirow{6}{*}{Gel/Suc/W0} & \multirow{6}{*}{\begin{tabular}{c} \(N_{\text{valid}}=52\) \\ \(T=474.5\) \\ \(Z=1.953\) \\ \(p=0.05\) \\ \end{tabular} } & \multirow{6}{*}{\
\begin{tabular}{c} \(N_{\text{valid}}=55\) \\ \(T=412.0\) \\ \(Z=3000\) \\ \(p=0.003\) \\ \end{tabular} } & \multirow{6}{*}{
\begin{tabular}{c} Bet/Gly/Suc/W6 \\ \(T=408.0\) \\ \(Z=2.104\) \\ \(p=0.035\) \\ \end{tabular} } \\ \cline{2-4}  & & & & \\ \cline{2-4}  & & & \\ \hline Pro/Gly/Sor/W0 & & & Pro/Gly/Sor/W3 & \\ \hline \multirow{6}{*}{Gly/Glu/Sor/W0} & \(N_{\text{valid}}=49\) & & \(N_{\text{valid}}=51\) & \\  & \(T=420.5\) & & \(T=292.0\) & \\  & \(Z=1.910\) & & \(Z=3.478\) & \\  & \(p=0.06\) & & \(p=0.005\) & \\ \hline Gly/Glu/Sor/W0 & & Gly/Glu/Sor/W3 & & Gly/Glu/Sor/W6 \\ \hline \multirow{6}{*}{Gel/Sor/W0} & \(N_{\text{valid}}=50\) & & \(N_{\text{valid}}=49\) & \\  & \(T=554.0\) & & \(T=315.0\) & \\  & \(Z=0.806\) & & \(Z=2.959\) & \\  & \(p=0.420\) & & \(p=0.003\) & \\ \hline Bet/EG0 & & & Bet/EG3 & \\ \cline{2-4}  & \(N_{\text{valid}}=48\) & & \(N_{\text{valid}}=49\) & \\ \cline{2-4}  & \(T=493.5\) & & \(T=398.5\) & \\ \cline{2-4}  & \(Z=0.969\) & & \(Z=2.129\) & \\ \cline{2-4}  & \(p=0.033\) & & \(p=0.033\) & \\ \hline \hline \end{tabular}
\end{table} 
Table 5: Wilcoxon matched pairs test results (\(p=0.01\))—the colored cells between the columns symbolize the statistically significant (red color) or non-significant (green color) difference between the samples. The improved stability of the components dispersed in individual NADES compared to the control after 6 months of storage at room temperature is evident. A possible explanation for the better stability of the CO2-NADES systems compared to the control is that NADES played the role of a physical predictor of the components, which, consequently, ensured the protection of the components from oxidative degradation. Potentially, the high viscosity of Gly/Glu (5258.47 \(\pm\) 322.86 mPa\(\cdot\)s) and Bet/Gly/Suc/W (2246.77 \(\pm\) 45.27 mPa\(\cdot\)s) could be one of the factors that contributed to the stabilization. In a previous study, it had been suggested that stabilization in high-viscous NADES can contribute to the preservation due to the reduced movement of the molecules [21]. However, in our study, no clear relationship between viscosity and stability was noticed because Bet/EG (48.63 \(\pm\) 0.42 mPa\(\cdot\)s) with a very low viscosity proved to be one of the NADES with stabilizing capacity. Furthermore, the stabilizing capacity of NADES may be due to the development of intermolecular interactions with individual components of _S. montana_[11; 21]. Namely, _S.