## 3 Results and Discussion
### Biomass Production, Sugars and Nitrogen Consumption
rotunda_**} & & & & & & & \\ \hline \multicolumn{2}{c}{**Substrate**} & \multicolumn{2}{c}{**Time**} & \multicolumn{2}{c}{**X**} & \multicolumn{2}{c}{**TScon**} & \multicolumn{2}{c}{**Ncon**} & \multicolumn{2}{c}{**YVS**} & \multicolumn{2}{c}{**EPS**} & \multicolumn{2}{c}{**IPS**} & \multicolumn{2}{c}{**IPS**} \\ \multicolumn{2}{c}{**(Days)**} & \multicolumn{2}{c}{**(g/L)**} & \multicolumn{2}{c}{**(s)**} & \multicolumn{2}{c}{**(\%)**} & \multicolumn{2}{c}{**(\%)**} & \multicolumn{2}{c}{**(\%)**} & \multicolumn{2}{c}{**(g/g)**} & \multicolumn{2}{c}{**(g/L)**} & \multicolumn{2}{c}{**(g/L)**} & \multicolumn{2}{c}{**(\%,w/w)**} \\ \hline \multicolumn{2}{c}{**Static**} & \multicolumn{2}{c}{**Static**} & & & & & & \\ \hline \multicolumn{2}{c}{} & 5 & d & 0.87 \(\pm\) 0.01 & 21.0 \(\pm\) 0.1 & 15.3 \(\pm\) 0.1 & 0.24 \(\pm\) 0.01 & **0.94 \(\pm\) 0.01** & 0.13 \(\pm\) 0.02 & 15.0 \(\pm\) 0.3 \\ \multicolumn{2}{c}{} & 10 & c & 5.67 \(\pm\) 0.73 & 49.1 \(\pm\) 0.0 & 61.6 \(\pm\) 2.1 & **0.68 \(\pm\) 0.05** & 0.67 \(\pm\) 0.06 & 1.43 \(\pm\) 0.06 & 25.3 \(\pm\) 0.9 \\ \multicolumn{2}{c}{} & 19 & a, b, e, f & **7.29 \(\pm\) 0.16** & **80.5 \(\pm\) 1.4** & **97.4 \(\pm\) 0.2** & 0.53 \(\pm\) 0.16 & 0.28 \(\pm\) 0.01 & **2.04 \(\pm\) 0.03** & **28.0 \(\pm\) 0.4** \\ \hline \multicolumn{2}{c}{} & 8 & c, d & 5.22 \(\pm\) 0.06 & 45.9 \(\pm\) 0 & 82.4 \(\pm\) 0.1 & **0.57 \(\pm\) 0.06** & **0.87 \(\pm\) 0.22** & 0.84 \(\pm\) 0.06 & 16.0 \(\pm\) 0.6 \\ \multicolumn{2}{c}{} & 16 & c, f & 6.24 \(\pm\) 0.05 & 66.5 \(\pm\) 1.3 & 90.2 \(\pm\) 2.1 & 0.48 \(\pm\) 0.05 & 0.37 \(\pm\) 0.01 & 2.63 \(\pm\) 0.14 & **2.2 \(\pm\) 2.2** \\ \multicolumn{2}{c}{} & 35 & a, b & **7.39 \(\pm\) 0.27** & **85.2 \(\pm\) 1.1** & **97.5 \(\pm\) 0.1** & 0.43 \(\pm\) 0.27 & 0.13 \(\pm\) 0.01 & 1.85 \(\pm\) 0.04 & 25.0 \(\pm\) 0.5 \\ \hline \multicolumn{2}{c}{} & 5 & d & 0.62 \(\pm\) 0.05 & 16.9 \(\pm\) 1.3 & 11.7 \(\pm\) 0.1 & 0.17 \(\pm\) 0.06 & **1.66 \(\pm\) 0.03** & 0.09 \(\pm\) 0.11 & 15.0 \(\pm\) 1.1 \\ \multicolumn{2}{c}{} & 10 & c & 9.69 \(\pm\) 0.01 & 50.2 \(\pm\) 1.7 & 56.7 \(\pm\) 0.2 & **0.88 \(\pm\) 0.01** & 1.37 \(\pm\) 0.08 & 2.46 \(\pm\) 0.11 & 25.4 \(\pm\) 1.1 \\ \multicolumn{2}{c}{} & 14 & c, f & 12.65 \(\pm\) 0.38 & 77.5 \(\pm\) 1.0 & 83.5 \(\pm\) 0.9 & 0.74 \(\pm\) 0.05 & 0.97 \(\pm\) 0.06 & 3.34 \(\pm\) 0.09 & 26.4 \(\pm\) 0.7 \\ \multicolumn{2}{c}{} & 19 & a, b & **13.46 \(\pm\) 0.02** & **95.2 \(\pm\) 0.08** & **88.9 \(\pm\) 0.3** & 0.64 \(\pm\) 0.27 & 0.42 \(\pm\) 0.06 & 2.92 \(\pm\) 0.06 & 21.7 \(\pm\) 0.4 \\ \hline \multicolumn{2}{c}{} & 8 & d & 1.80 \(\pm\) 0.23 & 17.0 \(\pm\) 1.7 & 23.3 \(\pm\) 1.9 & 0.26 \(\pm\) 0.06 & **3.94 \(\pm\) 0.07** & 0.29 \(\pm\) 0.10 & 16.0 \(\pm\) 1.2 \\ \multicolumn{2}{c}{} & 12 & c & 7.93 \(\pm\) 0.10 & 28.9 \(\pm\) 2.4 & 37.2 \(\pm\) 2.1 & **0.67 \(\pm\) 0.01** & 3.77 \(\pm\) 0.03 & 1.40 \(\pm\) 0.05 & 17.7 \(\pm\) 0.6 \\ \multicolumn{2}{c}{} & 35 & a, b, e, f & **12.01 \(\pm\) 0.04** & **90.1 \(\pm\) 1.9** & **69.8 \(\pm\) 0.3** & 0.50 \(\pm\) 0.27 & 3.26 \(\pm\) 0.11 & **2.14 \(\pm\) 0.22** & **17.8 \(\pm\) 1.8** \\ \hline \multicolumn{2}{c}{} & \multicolumn{2}{c}{**Agitated**} & & & & & & \\ \hline \multicolumn{2}{c}{} & 5 & d & 0.90 \(\pm\) 0.01 & 30.4 \(\pm\) 0.0 & 25.4 \(\pm\) 2.2 & 0.17 \(\pm\) 0.01 & **1.09 \(\pm\) 0.05** & 0.15 \(\pm\) 0.07 & 16.7 \(\pm\) 0.2 \\ \multicolumn{2}{c}{} & 17 & c & 7.71 \(\pm\) 0.13 & 85.3 \(\pm\) 1.3 & 73.1 \(\pm\) 0.01 & **0.53 \(\pm\) 0.13** & 0.27 \(\pm\) 0.01 & 1.53 \(\pm\) 0.01 & 19.8 \(\pm\) 0.2 \\ \multicolumn{2}{c}{} & 19 & a, b, e, f & **7.95 \(\pm\) 0.65** & **90.0 \(\pm\) 1.2** & **97.4 \(\pm\) 0.04** & 0.52 \(\pm\) 0.65 & 0.25 \(\pm\) 0.01 & **2.40 \(\pm\) 0.02** & **30.2 \(\pm\) 0.3** \\ \hline \multicolumn{2}{c}{} & 8 & c, d & 3.37 \(\pm\) 0.47 & 18.3 \(\pm\) 0.1 & 41.4 \(\pm\) 0.0 & **0.92 \(\pm\) 0.47** & **0.64 \(\pm\) 0.03** & 0.44 \(\pm\) 0.02 & 13.0 \(\pm\) 0.23 \\ \multicolumn{2}{c}{} & 16 & f & 6.26 \(\pm\) 0.17 & 70.8 \(\pm\) 2.9 & 75.1 \(\pm\) 0.8 & 0.44 \(\pm\) 0.17 & 0.42 \(\pm\) 0.04 & 1.85 \(\pm\) 0.14 & **29.5 \(\pm\) 2.3** \\ \multicolumn{2}{c}{} & 28 & e & 8.63 \(\pm\) 0.75 & 80.4 \(\pm\) 0.9 & 96.9 \(\pm\) 1.1 & 0.54 \(\pm\) 0.75 & 0.34 \(\pm\) 0.