## 3 Materials and Methods
### Magnetic Resonance Imaging  
Magnetic resonance imaging (MRI) equipment provides highly detailed images of living tissues, making it invaluable for both brain and body human studies. The technology behind MRI allows for the visualization of internal structures with remarkable clarity, enabling healthcare professionals to diagnose and monitor a wide range of medical conditions. Among the diverse applications of MRI, data obtained from these images are commonly used for detecting tissue deformities such as cancers, injuries, and other abnormalities. This capability is critical for early diagnosis and effective treatment planning, as it allows for the precise localization and characterization of pathological changes in tissues [28]. MRI equipment is composed of several key components that work together to generate these detailed images. First, there is the patient support and magnet system (Figure 1a), where the patient lies down during the examination. The magnet creates a strong magnetic field, which is essential for the imaging process. Inside this magnet, radiofrequency coils are used to send and receive signals from the body. The interaction between the magnetic field and these signals is what creates the detailed images. Figure 1: RM equipment. (**a**) Magnet: structure where the patient is inserted during the exam. (**b**) Console: used by the operator to perform the exam and monitor the patient. (**c**) Example of image generated by magnetic resonance of a hand. In addition to the patient support and magnet system, there is a console (Figure 1b), which is used by the operator to control the acquisition of the exam. This console is equipped with sophisticated software that allows the operator to adjust various parameters of the MRI scan, ensuring that the images produced are of the highest quality and most useful for diagnostic purposes. The operator can also view the images in real time, making adjustments as needed during the scan. An example of an image generated using MRI technology is shown in Figure 1c. This image illustrates the level of detail that can be achieved, highlighting the fine structures within the tissues being examined. Such images are invaluable for medical professionals, providing them with the information needed to make informed decisions about patient care. Whether it is for identifying a small tumor, assessing the extent of an injury, or monitoring the progress of a disease, MRI continues to be a cornerstone of modern medical imaging. In the case of brain MRI scans, three primary plane orientations are considered for imaging: sagittal, coronal, and transverse. Each of these planes provides unique and complementary views of the brain's structure, enabling comprehensive analysis and diagnosis. The sagittal plane divides the brain into left and right sections. During an MRI scan, the lower extremities of the corpus callosum are used to determine the slope of the images that will be generated. The corpus callosum, a thick band of nerve fibers, connects the right and left hemispheres of the brain, playing a crucial role in interhemispheric communication. The equipment operator uses these structures to align the line segment that delimits the area of interest, providing essential guidance for the formation of other images. This process is known as slice planning, as illustrated in Figure 2a. Proper alignment ensures that the resulting images accurately represent the anatomical features of interest, facilitating accurate diagnosis and treatment planning. The coronal plane slices the brain into front (anterior) and back (posterior) sections. This orientation is particularly useful for examining the brain's frontal and occipital lobes, as well as the structures located in between. By analyzing coronal images, medical professionals can assess conditions such as tumors, hemorrhages, and structural abnormalities. This view is crucial for evaluating the integrity of brain structures and understanding their spatial relationships.