## 1 Introduction
Their lipid composition can also distinguish mitochondrial inner and outer membranes. The outer mitochondrial membrane is a smooth lipid-rich envelope with pore-forming proteins. In contrast, the highly folded inner mitochondrial membrane is protein-rich harboring mainly enzymes of the respiratory chain [15]. PI is present in the outer mitochondrial membrane at a considerable amount. By contrast, CL and PE are enriched in the inner mitochondrial membrane. The presence of high CL levels in the mitochondrial membranes suggests that this PL is essential for efficient oxidative phosphorylation. The unsaturation degree of membrane lipids was found to influence respiratory properties and cytochrome content of mitochondria from _S. cerevisiae_. It was shown that a decrease in the amount of unsaturated FAs also declined the activity of several mitochondrial enzymes. [PERSON]'s team has methodically studied the relationship between the mitochondria lipidome and the life span of the yeast. The authors declared that ageing changes of the mitochondria lipid composition in the yeast include. (1) The levels of PE, CL and monolysocardiolipin (MLCL) decrease in the mitochondrial membranes. (2) the levels of phosphatidic acid (PA), PS, PC and phosphatidylglycerol (PG) increase in the mitochondrial membranes [17; 18]. The applied lithoholic bile acid was reported to penetrate into the mitochondria and accumulate in the inner mitochondria membrane. This leads to changes in the phospholipid composition of the mitochondria membranes [17; 18; 19]. The remodeling of mitochondrial phospholipids intensifies while a yeast culture ages and finally causes substantial changes in mitochondria membrane lipidome. The composition of the mitochondria's respiratory chain in traditional yeast species, namely _S. cerevisiae_ is quite invariable. In particular, their mitochondria have no complex I [20]. By contrast, the _Endomyes magnusii_ yeast being an obligate aerobe possesses a complete respiratory system similar in its mitochondrial system to the animal one. The _E. magnusii_ cannot grow in the presence of the inhibitors of the mitochondria transcription (ethidium bromide) and mitochondria translation (erythromycin). Under these conditions, the growth should undergo only at the expense of substrate and glycolytic phosphorylation. In our experiments, the glycerol-containing medium-low concentrations of ethidium bromide (5-15 ug/mL) led to the complete inhibition of the growth. We assayed the number of generations (Figure S3B). We could see similar results in the experiments with erythromycincin, which in low concentration (5 mg/mL) blocked the growth of the _E. magnusii_ (Figure S3A). In the yeast with fermentative metabolism types such as _S. cerevisiae_, the membrane and mitochondrial apparatus are ill-developed. There are only scanty large mitochondria with small cristae. Their mitochondria have much fewer cristae and trend to irregularity in their shape, structure, and packing. However, the yeast of strongly-pronounced aerobic metabolism, namely _E. magnusii_, possesses well-developed membrane apparatus with abundant complex mitochondria with numerous cristae [21]. According to the above-mentioned statement, we consider that the _E. magnusii_ species is a unique model close to the animal in the mitochondrial system that makes it a convenient model for studying some mechanisms, including ageing. Previously, we demonstrated high viability capacity in the _E. magnusii_ yeast upon chronological ageing [22]. For the 20-day cultivation of the strain in glycerol-containing (1%) medium, there were no signs of growth culture inhibition. The analysis of cell survival during culture ageing showed that after 168 h of growth (7 days), it decreased by not more than 10-15% compared to the logarithmic growth stage [22].