## 4 Conclusions  
In this study, a simultaneous RP-HPLC and LC-MS/MS MRM analysis methods for efficient quality control of DGJT, which is effective in postoperative intestinal obstruction and postoperative constipation, was developed and verified through linearity, LOD, LOQ, accuracy, and precision. These results provide a framework for quality control monitoring  
\begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline \multirow{2}{*}{**Compound**} & \multicolumn{6}{c}{**Content (mg/g)**} \\ \cline{2-7}  & \multicolumn{2}{c}{**Batch 1**} & \multicolumn{2}{c}{**Batch 2**} & \multicolumn{2}{c}{**Batch 3**} & \multirow{2}{*}{**Source**} \\ \cline{2-7} \cline{5-7}  & **Mean \(\pm\) SD** & **RSD (\%)** & **Mean \(\pm\) SD** & **RSD (\%)** & **Mean \(\pm\) SD** & **RSD (\%)** \\ \hline Hyperoside & 0.95 \(\pm\) 0.01 & 0.75 & 0.96 \(\pm\) 0.01 & 0.77 & 0.97 \(\pm\) 0.01 & 0.55 & _Z. schinfolium_ \\ Querctrin & 3.85 \(\pm\) 0.03 & 0.76 & 3.88 \(\pm\) 0.03 & 0.73 & 3.88 \(\pm\) 0.02 & 0.41 & _Z. schinfolium_ \\ Ginsnoside Rg1 & 13.53 \(\pm\) 0.18 & 1.36 & 13.85 \(\pm\) 0.23 & 1.64 & 13.86 \(\pm\) 0.16 & 1.14 & _P. ginseng_ \\
6-Gingrol & 1.44 \(\pm\) 0.00 & 0.23 & 1.45 \(\pm\) 0.01 & 0.48 & 1.46 \(\pm\) 0.02 & 1.40 & _Z. officiale_ \\ \hline \hline \end{tabular}
\end{table}
Table 4: Content of the four marker analytes in DGJT sample determined using HPLC (\(n\) = 3).  
Figure 2: Total ion chromatograms of the standard solution (**A**) and DGJT sample (**B**) by LC-MS/MS MRM method. Hyperoside (**1**), querctrin (**2**), ginseng Rg1 (**3**), and 6-gingerol (**4**).  
of DGJT as well as other traditional Korean medicines, traditional Chinese medicines, and Kampo medicines.  
The following are available online at [[https://www.mdpi.com/article/10.3390/app11083437/s1](https://www.mdpi.com/article/10.3390/app11083437/s1)]([https://www.mdpi.com/article/10.3390/app11083437/s1](https://www.mdpi.com/article/10.3390/app11083437/s1)), Figure S1: Chemical structures of the four marker analytes used in the quantitative analysis of DGJT; Figure S2: HPLC chromatogram of constituent herbal medicine and its main components. A: _Z. officinal_, _B. P. ginseng_, and C: _Z. schintifolium_; Figure S3: HPLC chromatograms of the standard solution (**A**) and 70% methanol extract of DGJT sample (**B**). Xeroboside (**1**), rutin (**2**), hyperoside (**3**), ferulic acid (**4**), foniculin (**5**), quercitrin (**6**), isorhametin-7-glucoside (**7**), ginseng Rg1 (**8**), ginseng Rb1 (**9**), 3,4,5-trihydroxy-7,4'-dimethoxyoxyflavone (**10**), dictammin (**11**), scinifolin (**12**), 6-gingerol (**13**); Figure S4: Extracted ion chromatograms of the reference standard (**A**) and DGJT sample (**B**) by LC-MS/MS MRM mode. Table S1: Information on the composition of DGJT; Table S2: HPLC parameters for quantification of DGJT; Table S3: MRM parameters for LC-MS/MS analysis of 4 marker compounds in DGJT. Table S4: System suitability for the four marker analytes in the developed HPLC method; Table S5: Repeatability for retention time and peak areas of the four marker analytes using HPLC (\(n=6\)). Table S6: The linear range, regression equation, \(r^{2}\), LODs, and LOQs of the analytes from YPS using LC-MS/MS (\(n=3\)).  
Conceptualization, C.-S.S. and H.-K.S.; performing experiments and analyzing data, and writing--original draft preparation, C.-S.S.; funding acquisition, H.-K.S. All authors have read and agreed to the published version of the manuscript.  
This research was supported by a grant from the Korea Institute of Oriental Medicine (grant number KSN2021310).  
Not applicable.  
The data presented in this study are available in the article (tables and figures).  
The authors have declared no conflict of interest.