# Feedback Modulation between Human IN080 Chromatin Remodeling Complex and miR-372 in HCT116 Cells  
[PERSON]  
1 School of Life Sciences, Jilin University, Changchun 130012, China; [EMAIL_ADDRESS] (J.A.S. ); [EMAIL_ADDRESS] (Y.M. ); [EMAIL_ADDRESS] (J.C.); [EMAIL_ADDRESS] (W.C.); [EMAIL_ADDRESS] (Q.Z. ); [EMAIL_ADDRESS] (C.C.) [PERSON]  
1 School of Life Sciences, Jilin University, Changchun 130012, China; [EMAIL_ADDRESS] (J.A.S. ); [EMAIL_ADDRESS] (Y.M. ); [EMAIL_ADDRESS] (J.C.); [EMAIL_ADDRESS] (W.C.); [EMAIL_ADDRESS] (Q.Z. ); [EMAIL_ADDRESS] (C.C.) [PERSON]  
1 School of Life Sciences, Jilin University, Changchun 130012, China; [EMAIL_ADDRESS] (J.A.S. ); [EMAIL_ADDRESS] (Y.M. ); [EMAIL_ADDRESS] (J.C.); [EMAIL_ADDRESS] (W.C.); [EMAIL_ADDRESS] (Q.Z. ); [EMAIL_ADDRESS] (C.C.) [PERSON]  
1 School of Life Sciences, Jilin University, Changchun 130012, China; [EMAIL_ADDRESS] (J.A.S. ); [EMAIL_ADDRESS] (Y.M. ); [EMAIL_ADDRESS] (J.C.); [EMAIL_ADDRESS] (W.C.); [EMAIL_ADDRESS] (Q.Z. ); [EMAIL_ADDRESS] (C.C.) Qingzhi Zhao  
1 School of Life Sciences, Jilin University, Changchun 130012, China; [EMAIL_ADDRESS] (J.A.S. ); [EMAIL_ADDRESS] (Y.M. ); [EMAIL_ADDRESS] (J.C.); [EMAIL_ADDRESS] (W.C.); [EMAIL_ADDRESS] (Q.Z. ); [EMAIL_ADDRESS] (C.C.) Chengyu Cai  
1 School of Life Sciences, Jilin University, Changchun 130012, China; [EMAIL_ADDRESS] (J.A.S. ); [EMAIL_ADDRESS] (Y.M. ); [EMAIL_ADDRESS] (J.C.); [EMAIL_ADDRESS] (W.C.); [EMAIL_ADDRESS] (Q.Z. ); [EMAIL_ADDRESS] (C.C.) [PERSON]  
MicroRNAs (miRNAs) are 19-22 nucleotide non-coding single-stranded RNAs in plants, animals, and viruses [5]. It can post-transcriptionally regulate gene expression by controlling translation or mRNA degradation [6]. As the major regulators of gene expression, miRNAs have been discovered to control many important biological processes, such as cell proliferation, apoptosis, and migration [7]. Furthermore, numerous studies have shown that the imbalanced expression of miRNAs may result in the aberrant expression of target proteins, which can affect numerous biological processes in cells. According to this viewpoint, abnormal miRNA expression has been seen in a variety of malignancies, including gastric [8], lung [9], and prostate cancer (PCa) [10], indicating that miRNAs play a crucial role in the development of cancer. MiRNA transcriptional silencing is a well-known mechanism of epigenetic alterations in cancer. Among the silenced miRNAs, miR-372 and miR-373 have been linked to DNA hypermethylation. However, it is worth mentioning that histone modifications may also potentially influence their expression. Inhibitors of histone deacetylase (HDAC), such as suberoylanilide hydroxamic acid (SAHA) and trichostatin A (TSA), can restore the expression of miR-373 in LNSC A549 and Calu-6 cells. This restoration inhibits cell proliferation and invasion by decreasing the expression of miR-373 target genes, interleukin-1 receptor-associated kinase 2 (_IRAK2_), and lysosomal-associated membrane protein 1 (_LAMPI_) [11]. On the other hand, miR-520 and miR-373 are responsible for the upregulation of matrix metalloproteinase 9 (_MMP9_) in human fibrosarcoma HT1080 cells by directly targeting the 3\({}^{\prime}\)-UTR of mTOR and sirutin 1 (_SIRT_) mRNAs. This upregulation activates the Ras/Raf/MEK/ Erk signaling pathway and NF-B, increasing cell growth [12]. According to the literature, human embryonic stem cells (ESCs) contain microRNA-371-373 (miR-371-373) gene clusters in a highly abundant form, indicating that miR-371-373 genes are important in the maintenance of stemness in ESCs [13]. Through this, the miR-371-373 gene cluster, involved in signaling pathways, such as the Wnt/ \(\beta\)-catenin pathway, can promote stem cell self-renewal and oncogenesis in various tissues [14]. For instance, miR-372 and miR-373 promote the stemness of CRC cells by suppressing the expression of differentiation genes, such as the nuclear factor kappa B (_NF-kB_), mitogen-activated protein kinase (_MAPK_)/ extracellular signal-regulated kinase (_Erk_), and vitamin D receptor (_VDR_) [15].