## 2 Materials and Methods
### Synthesis
CH) 7.10 (t, \(J\) = 7.03 Hz, 1H, Hp), 7.20 (t, \(J\) = 7.53 Hz, 2H, H\({}_{0}\)), 7.29 (d, \(J\) = 7.28 Hz, 2H, H\({}_{0}\)), 7.70-7.81 (m, 2H, H9, H8), 7.87 (d, \(J\) = 7.53 Hz, 1H, H7), 8.01 (d, \(J\) = 7.28 Hz, 1H, H10), 9.68 (s, 1H, NH). \({}^{13}\)C NMR \(\delta\) (ppm): 14.4 (SCH\({}_{3}\)), 30.0 (NCH\({}_{3}\)), 35.3 (C5), 117.4 (Ca), 125.6 (C10), 125.8 (C7), 126.4 (Cp), 128.0 (Co), 128.6 (Cm), 130.3 (C6a), 131.8 (C10a), 133.2 (C8), 134.7 (C9), 139.2 (C5a), 145.0 (Ci), 149.7 (C2), 160.2 (C4, C=O), 161.6 (C12a), 179.1 (C=O), 181.5 (C=O). MS: (70 eV) \(m/z\) (%) = 414 (11, M\({}^{+}\)), 337 (100). Anal. Calcd. for C\({}_{23}\)H\({}_{17}\)N\({}_{3}\)O\({}_{3}\)S C, 66.49; H, 4.12; N, 10.11; found C, 66.47; H, 4.15; N, 10.12. _3-methyl-2-(methylthio)-5-(4-methylphenyl)-5,12-dilhydrobenzo[g]pyrimidine[4,5-b]quinoline-4,6,11(3H)-trione_ (**4j**). Red solid. 81%. m.p. 27 \({}^{\circ}\)C. IR (KBr, v cm\({}^{-1}\)), 3227 (N-H st), 1647 (C=O,34.4 (C5), 54.9 (OCH\({}_{3}\)), 113.5 (Co), 117.7 (C4a), 125.6 (C10), 128.8 (C\(m\)), 130.3 (C6a), 131.8 (C\(l\)), 133.1 (C7), 134.7 (C9), 137.3 (C10a), 138.9 (C5a), 149.6 (C2), 157.8 (C2), 160.2 (C=O), 161.4 (C12a). MS: (70 eV) \(m/z\) (%) = 445 (45, M\({}^{+}\)), 337 (100). Anal. Calcd. for C\({}_{24}\)H\({}_{19}\)N\({}_{3}\)O\({}_{4}\)S C, 64.71; H, 4.30; N, 9.43; found C, 64.75; H, 4.27; N, 9.45. _3-methyl-2-(methylthio)-5-(3,4,5-trimethoxyphenyl)-5,12-dihydrobenzo[g]pyrimindo[4,5-b]quinoline-4,6,11(3H) -trione_ (**41**). Brown solid. 80%. m.p. 263 \({}^{\circ}\)C. IR (KBr, \(\upsilon\) cm\({}^{-1}\)), 3243 (NH st), 1648 (C=O st). \({}^{1}\)H NMR (400 MHz, DMSO-\(d_{6}\)) \(\delta\) (ppm): 2.62 (s, 3H, SCH\({}_{3}\)), 3.33 (s, 3H, NCH\({}_{3}\)), 3.56 (s, 3H, OCH\({}_{3}\)), 3.67 (s, 6H, OCH\({}_{3}\)), 5.21 (s, 1H, H5), 6.57 (s, 2H, Ho) 7.78-7.82 (m, 2H, H8, H9), 7.91 (d, \(J\) = 7.28 Hz, 1H, H7), 8.03 (d, \(J\) = 7.28 Hz, 1H, H10), 9.60 (s, 1H, NH). \({}^{13}\)C NMR \(\delta\) (ppm): 14.4 (SCH\({}_{3}\)), 29.9 (NCH\({}_{3}\)), 35.6 (C5), 55.8 (OCH\({}_{3}\)), 59.7 (OCH\({}_{3}\)), 105.6 (Co), 117.1 (C4a), 125.6 (C7), 125.8 (C10), 130.4 (C6a), 131.8 (C10a), 133.1 (C8), 134.6 (C9), 136.5 (C5a), 140.6 (Ci), 149.7 (C2), 152.5 (C11a), 161.5 (C12a), 179.1 (C=O), 181.6 (C=O). MS: (70 eV) \(m/z\) (%) = 505 (76, M\({}^{+}\)), 474 (15), 337 (100). Anal. Calcd. for C\({}_{26}\)H\({}_{23}\)N\({}_{3}\)O\({}_{6}\)S C, 61.77; H, 4.59; N, 8.31; found C, 61.79; H, 4.62; N, 8.34. _3-methyl-2-(methylthio)-5-(4-trifluoromethylphenyl)-5,12-dihydrobenzo[g]pyrimidine_[4,5-b]quinoline-4,6,11(3H) -trione_ (**4m**). Red solid. 75%. m.p. \(>\)300 \({}^{\circ}\)C. IR (KBr, \(\upsilon\) cm\({}^{-1}\)), 3447 (NH st), 1687 (C=O st), 1519 (C=C st). \({}^{1}\)H NMR (400 MHz, DMSO-\(d_{6}\)) \(\delta\) (ppm): 2.74 (s, 3H, SCH\({}_{3}\)), 2.88 (s, 3H, NCH\({}_{3}\)), 5.33 (s, 1H, H5), 7.39 (d, \(J\) = 7.03 Hz, 1H, H7), 7.56 (d, \(J\) = 7.06 Hz, 2H, Ho), 7.72-7.74 (d, \(J\) = 7.03 Hz, 2H, H\({}_{7}\)), 7.80 (t, 1H, H8), 7.89 (t, 1H, H9), 8.03 (d, \(J\) = 7.03 Hz, 1H, H10), 9.88 (s, 1H, NH). \({}^{13}\)C NMR \(\delta\) (ppm): 14.5 (SCH\({}_{3}\)), 29.9 (NCH\({}_{3}\)), 35.7 (C5), 99.5 (C4a), 111.6 (C5a), 127.0 (C10), 127.3 (Co), 128.0 (C7), 128.9 (_Cm_), 133.2 (C8), 135.4 (C9), 139.7 (Ci), 149.8 (C2), 157.3, 159.2 (Coa), 160.2 (C=O), 166.1 (C12a), 178.3 (C=O), 198.50 (C6). MS: (70 eV) \(m/z\) (%) = 480 (18, M\({}^{+}\)), 337 (100), 437 (40). Anal. Calcd. for C\({}_{24}\)H\({}_{16}\)F\({}_{3}\)N\({}_{3}\)O\({}_{3}\)S C, 59.62; H, 3.34; N, 8.69; found C, 59.65; H, 3.30; N, 8.73. _5-(benzo[d][1,3]dioxol-6-yl)-3-methyl-2-methylthio-5,12-dihydrobenzo[g]pyrimidine_[4,5-b]quinoline-4,6,11(3H) -trione_ (**4n**). Red solid. 75%. m.p. 254 \({}^{\circ}\)C. IR (KBr, \(\upsilon\) cm\({}^{-1}\)), 3225 (NH st), 1647 (C=O st), 1519 (C=C st).