## 3 Results
Simultaneously, the histogram (grey value) peaks defining the several CE organs were at a high level. \begin{table}
\begin{tabular}{l l l l l l} \hline \hline \multirow{2}{*}{**Stain, Exposure Time, Temperature**} & \multicolumn{2}{c}{**X-ray Density, HU**} & & & \\ \cline{2-6}  & **The Chick Embryo** & **Brain** & **Heart** & **Liver** & **Sclerotome (Neural Canal)** \\ \hline I2 KI (3\%), 24 h, 25 \({}^{\circ}\)C & 1089.1 \(\pm\) 50 & 1526.4 \(\pm\) 75 & 3503.4 \(\pm\) 129 & 6972.4 \(\pm\) 264 & 2991.6 \(\pm\) 140 \\ \hline I2 KI (3\%), 24 h, 40 \({}^{\circ}\)C & 839.6 \(\pm\) 47 & 957.3 \(\pm\) 43 & 3152.4 \(\pm\) 166 & 6979.3 \(\pm\) 348 & 3347.9 \(\pm\) 273 \\ \hline I2 KI (3\%), 48 h, 25 \({}^{\circ}\)C & 2845.1 \(\pm\) 235 & 3307.6 \(\pm\) 201 & 7228.8 \(\pm\) 301 & 15,525.0 \(\pm\) 699 & 8181.1 \(\pm\) 465 \\ \hline I2 KI (3\%), 48 h, 40 \({}^{\circ}\)C & 2483.8 \(\pm\) 178 & 2084.4 \(\pm\) 156 & 6570.1 \(\pm\) 435 & 13,575.0 \(\pm\) 621 & 7785.9 \(\pm\) 289 \\ \hline PMA (1\%), 24 h, 25 \({}^{\circ}\)C & 384.9 \(\pm\) 29 & 759.8 \(\pm\) 56 & 1629.0 \(\pm\) 91 & 3603.6 \(\pm\) 188 & 1680.4 \(\pm\) 110 \\ \hline PMA (1\%), 24 h, 40 \({}^{\circ}\)C & 635.1 \(\pm\) 42 & 1349.2 \(\pm\) 72 & 2120.9 \(\pm\) 99 & 4565.9 \(\pm\) 245 & 2080.9 \(\pm\) 130 \\ \hline PMA (1\%), 48 h, 25 \({}^{\circ}\)C & 472.8 \(\pm\) 29 & 810.5 \(\pm\) 44 & 1825.1 \(\pm\) 102 & 4138.9 \(\pm\) 203 & 1907.0 \(\pm\) 98 \\ \hline PMA (1\%), 48 h, 40 \({}^{\circ}\)C & 828.7 \(\pm\) 37 & 1544.0 \(\pm\) 128 & 2953.4 \(\pm\) 144 & 5685.5 \(\pm\) 276 & 2344.3 \(\pm\) 175 \\ \hline PTA (1\%), 24 h, 25 \({}^{\circ}\)C & 1652.7 \(\pm\) 95 & 1962.4 \(\pm\) 108 & 3992.9 \(\pm\) 264 & 8030.0 \(\pm\) 301 & 4716.8 \(\pm\) 234 \\ \hline PTA (1\%), 24 h, 40 \({}^{\circ}\)C & 2065.5 \(\pm\) 116 & 2395.9 \(\pm\) 148 & 5714.7 \(\pm\) 176 & 11,255.0 \(\pm\) 559 & 5744.5 \(\pm\) 212 \\ \hline PTA (1\%), 48 h, 25 \({}^{\circ}\)C & 1790.0 \(\pm\) 102 & 2258.2 \(\pm\) 190 & 4026.8 \(\pm\) 134 & 8611.1 \(\pm\) 301 & 5653.6 \(\pm\) 267 \\ \hline PTA (1\%), 48 h, 40 \({}^{\circ}\)C & 2362.3 \(\pm\) 157 & 2626.2 \(\pm\) 114 & 6185.8 \(\pm\) 312 & 10,816.0 \(\pm\) 489 & 5716.2 \(\pm\) 229 \\ \hline \hline \end{tabular}
\end{table} 
Table 3: X-ray density of various structures of CE (HH25-HH27 embryonic stage), n = 5. \begin{table}
\begin{tabular}{l c c c c c} \hline \hline \multirow{2}{*}{**Structures**} & \multicolumn{2}{c}{**X-ray Density, HU**} & & & \\ \cline{2-5}  & **4 Days** & **5 Days** & **6 Days** & **7 Days** & **8 Days** \\  & **(HH22–HH24)** & **(HH25–HH27)** & **(HH28–HH29)** & **(HH30–HH32)** & **(HH33–HH34)** \\ \hline The chick embryo & \(3115.9\pm 111\) & \(2065.5\pm 116\) & \(2447.7\pm 125\) & \(1517.2\pm 84\) & \(1807.9\pm 103\) \\ \hline Brain & \(5284.4\pm 202\) & \(2395.9\pm 148\) & \(2752.9\pm 112\) & \(2393.7\pm 135\) & \(3238.3\pm 193\) \\ \hline Eye (left) & \(7514,4\pm 257\) & \(3780.2\pm 180\) & \(3255.0\pm 177\) & \(1693.4\pm 95\) & \(1897.2\pm 98\) \\ \hline Heart & \(4923.2\pm 226\) & \(5714.7\pm 176\) & \(5401.1\pm 245\) & \(3513.8\pm 164\) & \(3563.9\pm 198\) \\ \hline Liver & \(9244.0\pm 378\) & \(11,255.0\pm 559\) & \(12,155.0\pm 589\) & \(5763.6\pm 243\) & \(6154.7\pm 302\) \\ \hline
\begin{tabular}{l} Sclerotome \\ (spine and neural canal) \\ \end{tabular} & \(6978.4\pm 320\) & \(5744.5\pm 212\) & \(6339.1\pm 369\) & \(2658.6\pm 139\) & \(2775.2\pm 173\) \\ \hline Mesoneph (left) & \(10,091.0\pm 461\) & \(8785.3\pm 342\) & \(9659.7\pm 401\) & \(4302.4\pm 157\) & \(4382.3\pm 189\) \\ \hline Stomach & \(13,873.0\pm 598\) & \(10,064.0\pm 497\) & \(9785.7\pm 389\) & \(4233.1\pm 187\) & \(4298.8\pm 194\) \\ \hline Lungs (left) & not defined & not defined & \(10,038\pm 450\) & \(3624.5\pm 143\) & \(2911.0\pm 157\) \\ \hline \end{tabular}
\end{table} 
Table 4: X-ray density of various structures of CE (HH22-HH34 embryonic stages) stained with 1% PTA solution for 24 h at 40 \({}^{\circ}\)C, n = 5.