# Voltailome and Essential Oil of _Ulomoides dermatoides_: A Broad-Spectrum Medical Insect
## 2 Results
### Obtention and Characterization of Essential Oils

Table 4: \triene, allopregnane, pimaric acid, 7-ethyl-1,4a,7-trimethyl-3,4,4b,5,6,8,10,10a-octahydro-2H-phenanthrene-1-carboxylic acid, isopimaric acid, 8-pimarenic acid, abiet-8-en-18-oic acid, 12\(\alpha\)-hydroxy-5\(\alpha\)-pregnane, coprostane, cholesterol, germanicol, 3-epimoretenol, campesterol, and avenasterol were found; carboxylic acid and derivatives: butanoic acid, valeric acid, peracetic, 10-undecenoic acid, tridecanoic acid, 2-resacrylic acid, 9-tetradecenoic acid, 9-hexadecenoic acid, _cis_-10-heptadecenoic acid, _cis_-11,14-eicosadienoic acid, _cis_-11-eicosenoic acid, arachidic acid, 1-monopalmitin, docosanoic acid, triacontadiene acid and dotriacontadiene acid; and alcohols: 2,2-dimethyl-3-pentanol, furfuryl alcohol, 2,3-butanediol, 4-hydroxybenzenemethanol, 2-pentadecanol, 1-heptadecanol, oleyl alcohol, 1-hexacosanol, 1-octacosanol and 1-dotriacontanol. In the derivatization for the detection of aldehydes and alkynes, a total of 12 aldehydes and three alkynes were identified. These corresponded to an increase in the total percentage of identified compounds of 0.01% and 0.56% for EOT1 and EOT2, respectively. In summary, the total percentages of identified compounds for EOT1 and EOT2 were 97.46% and 99.92%. The compounds exclusively found in EOT2 were eight of the 12 aldehydes and the three alkynes (Table 5). In general, the compounds obtained from both oils can be classified into 10 categories: alcohols, aldehydes, alkanes, alkenes, alkynes, alkyl disulfides, aromatic compounds, carboxylic acids, and their derivatives, quinones and terpen. The amount and type of these metabolites varied depending on the stimulus to which the sample was subjected when the essential oil was obtained. Despite a significant decrease in alkenes and terpenes with respect to the peak area, the variety of these metabolites in EOT2 increased. For the remaining metabolite groups, all compounds increased both in the peak area and in the variety of compounds present (Figure 1). One of the categories of greatest biological interest is terpenes; therefore, they were analyzed independently. In EOT2, the number of functionalized terpenes increased, and there was a tendency for the peak area to increase with respect to EOT1. Although in terpenes that were not functionalized, the areas of the peaks were smaller, the variety of terpenes present was greater in EOT2 than in EOT1 (Figure 2). \begin{table}
\begin{tabular}{l l c c c c c} \hline \hline  & \multicolumn{2}{c}{**Compounds**} & \multicolumn{2}{c}{**EOUd1**} & \multicolumn{2}{c}{**EOUd2**} & \multirow{2}{*}{**KI Ref**} \\  & & **RA (\%)** & & **KI Exp** & & **RA (\%)** & **KI Exp** & **KI Ref** \\ \hline  & Aldehydes & 0.01\% & & & 0.49\% & & \\
2 & Hexanal & 0.001 \(\pm\) 0.001 & 971.8 & 0.013 \(\pm\) 0.000 & 968.5 & 964 \\
3 & Heptanal & 0.003 \(\pm\) 0.000 & 1077.2 & 0.015 \(\pm\) 0.001 & 1077.2 & 1069 \\
4 & Benzaldehyde & & & 0.021 \(\pm\) 0.001 & 1107.6 & 1200 \\
1 & Phenylacetaldehyde & & & 0.161 \(\pm\) 0.001 & 1217.2 & 1194 \\
5 & Nonanal & 0.001\(\pm\)0.001 & 1278.5 & 0.041 \(\pm\) 0.001 & 1278.9 & 1267 \\
6 & Decanal & & & 0.014 \(\pm\) 0.002 & 1377.4 & 1366 \\
7 & Dodecanal & & & 0.013 \(\pm\) 0.000 & 1577.4 & \\
8 & Tridecanal & & & 0.011 \(\pm\) 0.003 & 1676.5 & \\
9 & Tetradecanal & & & 0.051 \(\pm\) 0.002 & 1774.8 & \\
10 & Pentadecanal & & & 0.012 \(\pm\) 0.001 & 1876.8 & \\
11 & Hexadecanal & & & 0.068 \(\pm\) 0.004 & 1976.9 & \\
12 & Octadecanal & & & 0.065 \(\pm\) 0.010 & 2177.4 & \\ \hline  & Alkynes & 0.00\% & & 0.07\% & & \\
1 & Pentadecine & & & 0.009 \(\pm\) 0.002 & 1744.4 & \\
2 & Hexadecine & & & 0.007 \(\pm\) 0.000 & 1849.8 & \\
3 & Octadecine & & & 0.054 \(\pm\) 0.002 & 2017 \\ \hline \hline \end{tabular}
\end{table} 
Table 5: Identified compounds of _U. dermateoides_ essential oils derivatized by acetal and enother reaction.