## 3 Results
### Optimization of Purification Conditions  
In the pursuit of optimizing purification conditions, extracts derived from the most efficacious extraction and partitioning methodology for tricyclazole--incorporating 0.1% FA in a MeCN/EA (1:1, _v/v_) mixture combined with the Original QuECherSR approach--were further processed. The purification efficiency was evaluated by treating these extracts with principal dSPE sorbents (PSA, C18, and PSA+C18) and PRiME HLB plus light (HLB). When PSA and C18 were individually used at a quantity of 25 mg (Methods No. 1 and 2), the average recoveries were recorded at 90.8% and 92.1%, respectively. Statistical analysis using ANOVA indicated no significant differences between the two single-sorbent methods (Table 2). HLB (No. 5) was shown to yield a competitive recovery rate of 90.9%, nearly mirroring the performance of single-use PSA, which supports its utility as an effective cleanup method for tricyclazole analysis. Interestingly, utilizing a combination of PSA and C18, with each at the same dosage (No. 3), afforded the highest recovery of 96.7% (_p_ < 0.05). This suggests a synergistic effect, with the dual-sorbent system enhancing the cleanup by effectively reducing matrix interferences more than when either sorbent was used independently. Conversely, doubling the amount of each sorbent to 50 mg each did not enhance the recovery (No. 4); the recovery rate observed was lower at 86.4% for the dSPE. The intended purpose of using sorbents like PSA and C18 is to selectively bind and remove contaminants and impurities from the sample matrix, thereby enhancing the purity of the analyte for subsequent analysis [21; 26; 27; 28]. However, an excess in sorbent quantity could introduce additional binding sites that remain unoccupied by impurities. This can result in the unintentional adsorption of tricyclazole molecules themselves, diminishing their presence in the final extract measured. The same extraction conditions were applied to ferimzone Z and its isomer E form, which also exhibited a decrease in recovery rates as the quantity of purification sorbent increased, so this further supports the notion [23]. The matrix effects were significantly influenced by the choice of dSPE sorbents and HLB cartridge used during the cleanup in brown rice (Table 2). The combination of PSA and C18 (No. 3 and 4) minimized matrix effects to a notable degree, resulting in values of \(-6.1\%\) to \(-6.2\%\), which indicates a highly efficient cleanup. In contrast, the other sorbents and conditions tested showed varying degrees of matrix interference. This highlights the importance of sorbent selection in reducing matrix effects, which is crucial for accurate quantification in residue analysis. The matrix effects appeared to be relatively unaffected by the increased amounts of the sorbents PSA and C18, when compared at 25 mg and 50 mg each. Despite the doubling of the sorbent mass, the matrix effect remained comparable, indicating that beyond a certain \begin{table}
\begin{tabular}{c c c c} \hline \hline \multirow{2}{*}{**No.**} & \multirow{2}{*}{**Purification Method (Major Sorbents)**} & **Recovery \(\pm\) sd 1** & **Matrix Effect \(\pm\) sd (\%)** \\  & & **(\%)** & **(\%)** \\ \hline
1 & dSPE (PSA, 25 mg) & 90.8 \(\pm\) 2.1 b 2  & \(-12.9\pm 2.4\) b \\ \hline
2 & dSPE (C18, 25 mg) & 92.1 \(\pm\) 1.3 b & \(-17.0\pm 1.9\) b \\ \hline
3 & dSPE (PSA, 25 mg and C18, 25 mg) & 96.7 \(\pm\) 1.1 a & \(-6.1\pm 0.3\) a \\ \hline
4 & dSPE (PSA, 50 mg and C18, 50 mg) & 86.4 \(\pm\) 1.4 c & \(-6.2\pm 2.6\) a \\ \hline
5 & PRiME HLB plus light & 90.9 \(\pm\) 1.6 b & \(-12.8\pm 0.9\) b \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Comparison of tricyclazole recovery rates and matrix effects using different dSPE sorbents and HLB cartridge cleanup in brown rice. threshold, additional sorbent does not necessarily correlate with increased interference reduction. The correct balance of sorbent properties is key to achieving optimal cleanup and ensuring the reliability of analytical results in complex sample matrices.