## 4 Discussion  
_P. emblica_ and _A. indica_ are a rich source of various biologically active compounds. Almost 15 biologically active compounds were reported in _P. emblica_ through Gas Chromatography-Mass Spectrometry (GC-MS) studies. Among these secondary metabolites present were 1,2,3 Benzenetriol, hexadecanoic acid, 9-octadecenoic acid, octadecenoic acid, tetraetracontane, and nonacosane [36]. In addition, several compounds were identified by ethyl acetate extract of phylumths emblica fruit using GC-MS analysis as having enormous medicinal potentialities [37]. These compounds include Citronellyl propionate, Citronellyl acetate, Capric acid methyl ester, 8-Amino-5-benzyloxy-6-methoxy-4-methylquinoline, Aciphyllene, Beta. -sistosterol, Hexacontanoic acid, 1-pentacontanol, N-Hexadecane, N-Pentadecane, Oleyl alcohol, Beta-sitostero acetate, Campesterol are active component of P emblica [37]. Moreover, several compounds are identified by ethyl acetate extract of phylumths emblica fruit using GC-MS as Citronellyl propionate, Citronelly acetate, Capric acid methyl ester, 8-Amino-5-benzyloxy-6-methoxy-4-methylquinoline, Aciphyllene, Beta. -sistosterol, Hexacontanoic acid, 1-pentacontanol, N-Hexadecane, Oleyl alcohol, Beta-sitostero acetate, Campesterol, active components of P emblica [37]. The methanolic extract of the leaves of Azadirachta indica was subjected to GC-MS analysis and different compounds were reported, such as 3,7,11,15-tetramethyll-2-hexadecen-1-ol; 9,12,15-Octadecatrienoic acid; \(\alpha\)-Linolenic acid), 8, 11, 14-Eicosatrienoic acid; N-Hexadecanoic acid, and Trileccanoic acid, the major components in the extract. Moreover, the methanolic extract of the leaves of Azadirachta indica by GC-MS analysis reported the compounds such as Phytol, Linolenic acid, Homo-\(\gamma\)-linolenic acid, Palmitic acid, and Tridecylic acid [38]. _P. emblica_ and _A. indica_ have various important medicinal properties and have been extensively studied previously, but there are gaps that still exist, such as examining the properties of these plants in a comprehensive manner and correlating the results about the special properties which it might possess. In this study, our focus was to investigate the anti-proteinase activity, membrane stabilizing potentials, antiglycating properties, and AGEs inhibition properties of these plants. As per our best knowledge, the properties of these plants have not been investigated earlier. However, we found a similarity in the results of our investigated properties of these plants with the reports of previous investigations. In earlier studies, researchers investigated different properties and found a concentration-dependent increase in the biological activities of extract of these plants. Similarly, we found that anti-inflammatory properties such as antihemolytic and anti-proteinase action, inhibition of protein denaturation, antiglycating, and anti-AGES formation properties were found to be increased in a dose-dependent manner. Oxidative stress and ROS induce the oxidative destruction of nucleic acids, protein, lipids, lipoproteins, carbohydrates, and connective tissue macromolecules [39]. The imbalance in the cellular oxidants production and their removal imposes oxidative stress. Several human diseases, especially heart disease, aging, arteriosclerosis, stroke, cancer, diabetes, inflammation, cataract, arthritis, muscular degeneration, and impaired wound healing, etc., are known to be associated with this damage [29]. It has been suggested that antioxidants can be better therapy for cancer prevention and treatment because of their ability to modulate the intracellular ROS levels [4]. Natural products containing phenolic compounds are very beneficial for human health because they can reduce and -scavenge free radicals such as ROS [40]. There are several incidences demonstrating the free radical scavenging ability of flavonoids as well as their ability to increase the antioxidant enzyme activities significantly [41].