# Comprehensive Analysis of the Acute Toxicity of Ionic Liquids Using Microtax(r) Bioassays
## 3 Results and Discussion
[13] is that the associated ion pair is less permeable into or through the cell membrane, and therefore, the active [FAP] anion cannot reach the cell, resulting in being less toxic or harmless as the alkyl chain size increases. Furthermore, following with the analysis of FAP-based ILs, the same interesting toxicity pattern also emerged with the increase in alkyl chain length on the FAP anion, with the values of EC\({}_{50}\) after 30 min of exposure of 50.54 mg/L and 5430 mg/L for C\({}_{4}\)C\({}_{1}\)Pyrr \begin{table}
\begin{tabular}{c c c c} \hline \hline
**IL** & **EC\({}_{10}\) 5 min/mg/L** & **EC\({}_{10}\) 15 min/mg/L** & **EC\({}_{10}\) 30 min/mg/L** \\  & **(Lower; Upper) Limits** & **(Lower; Upper) Limits** & **(Lower; Upper) Limits** \\ \hline C\({}_{2}\)C\({}_{1}\)Im TTSI & 44.54 (7.06; 82.03) & 18.24 (0.07; 38.18) & 8.94 (0.02; 22.05) \\ C\({}_{4}\)C\({}_{1}\)Im TTSI & 8.63 (0.00; 18.97) & 5.91 (0.00; 13.03) & 4.39 (0.00; 11.01) \\ C\({}_{4}\)C\({}_{1}\)C\({}_{1}\)Im TTSI [12] & 23.25 (0.00; 50.07) & 20.96 (7.96; 33.95) & 20.34(12.63; 28.05) \\ C\({}_{6}\)C\({}_{1}\)Im TTSI & 8.19 (6.42; 9.96) & 8.53 (6.91;10.16) & 12.46 (10.54; 14.37) \\ C\({}_{8}\)C\({}_{1}\)Im TTSI & 0.88 (0.80; 0.98) & 1.07 (0.83; 1.30) & 1.50 (0.84; 2.17) \\ AC\({}_{1}\)Im TTSI & 38.67 (2.85; 74.48) & 27.19 (3.07; 51.31) & 26.89 (4.41; 49.37) \\ AC\({}_{1}\)Im Cl & 174.31 (0.00; 435.31) & 172.68 (17.45; 327.90) & 150.85 (28.69; 273.00) \\ AC\({}_{1}\)Im DCA & 243.68 (91.39; 395.97) & 202.36 (124.59; 279.78) & 204.59 (133.20; 275.98) \\ C\({}_{4}\)C\({}_{1}\)Im FAP & 10.95 (3.62; 18.28) & 10.19 (4.96; 154.3) & 8.38 (3.86; 12.89) \\ C\({}_{1}\)C\({}_{1}\)Im DMP & 763.66 (679.73; 846.46) & 847.12 (794.31; 921.86) & 901.85 (45.03; 958.37) \\ C\({}_{2}\)Py TTSI & 39.23 (29.18; 49.29) & 27.55 (19.52; 35.59) & 24.32 (15.38; 33.26) \\ C\({}_{4}\)Py TTSI & 19.35 (0.00; 40.98) & 10.38 (0.00; 24.55) & 4.33 (0.00; 14.24) \\ C\({}_{6}\)Py TTSI & 14.30 (11.49; 17.11) & 13.27 (10.23; 16.31) & 17.15 (13.77; 20.53) \\ C\({}_{5}\)C\({}_{1}\)Pip TTSI & 42.26 (35.50; 49.01) & 37.00 (30.62; 43.38) & 37.41 (32.40; 42.41) \\ C\({}_{4}\)C\({}_{1}\)Pip TTSI & 33.61 (13.56; 53.97) & 24.51 (10.15; 318.50) & 22.10 (6.61; 37.60) \\ C\({}_{4}\)C\({}_{1}\)Pipr TTSI [12] & 438.08 (225.18; 650.98) & 254.32 (146.51; 362.18) & 170.23 (93.44; 247.12)) \\ C\({}_{6}\)C\({}_{1}\)Pyrr TTSI & 24.76 (18.44; 31.09) & 21.71 (16.15; 27.26) & 29.22 (21.09; 37.36) \\ C\({}_{8}\)C\({}_{1}\)Pyrr TTSI & 4.20 (2.59; 5.82) & 4.86 (2.88; 6.84) & 9.57 (3.44; 15.71) \\ C\({}_{4}\)C\({}_{1}\)Pyrr FAP & 295.81 (79.77; 511.84) & 270.28 (142.70; 397.86) & 23.992 (161.01; 318.83) \\ C\({}_{1}\)O\({}_{2}\)C\({}_{1}\)Pyrr FAP & 10.66 (0.81; 19.79) & 6.94 (1.09; 12.79) & 6.24 (2.26; 10.22) \\ C\({}_{4}\)C\({}_{1}\)Pyrr \({}_{4}\)FAP & 33.83 (13.75; 53.92) & 18.26 (2.88; 33.62) & 13.47 (10.4; 24.25) \\ C\({}_{4}\)C\({}_{1}\)Pyrr \({}_{5}\)FAP & - & - & 540.79 (114.86; 1000.97) \\ Phyt FAP & 445.54 (162.01; 729.08) & 224.03 (15.24; 457.16) & 190.91 (3.87; 305.99) \\ EAN [12] & 2304.89 (248.43; 4361.05) & 1609.79 (560.06; 3163.56) & 1517.65 (332.07; 2703.22) \\ PAN [23] & 2932.68 (2072.90; 3792.46) & 2138.10 (1402.95; 2873.26) & 2366.64 (1600.94; 3132.34) \\ BAN [23] & 136.59 (0.00; 354.65) & 160.30 (0.00; 369.51) & 139.74 (0.00; 341.90) \\ PEAN [23] & 203.54 (121.38; 285.71) & 182.43 (75.64; 289.23) & 183.21 (72.35; 294.07) \\ HEAN [23] & 36.14 (28.64; 43.65) & 25.76 (20.96; 30.57) & 24.98 (19.07; 30.89) \\ OAN [23] & 2.62 (0.53; 47.1) & 2.64 (0.87; 4.42) & 4.00 (1.36; 0.66) \\ C\({}_{2}\)Im NO\({}_{3}\)[12] & 100.10 (21.33; 179.99) & 103.80 (22.16; 184.19) & 127.39 (37.59; 214.25) \\ \hline \hline \end{tabular}
\end{table} 
Table 4: EC\({}_{10}\) effective concentration values in mg/L and the respective 95% confidence intervals, obtained after 5, 15, and 30 min of exposure of the marine bacteria _A. C4 FAP and C4C1 Pyrr C8 FAP, respectively. This unexpectable behavior is also consistent with the observations of [PERSON] et al. [11].