## 3 Results  
After 7 days of cultivation, the cell culture contained up to 40% stellate fibroblast-like cells attached to the polymer base (stromal progenitor cells) and up to 60% rounded and non-attached cells (hematopoietic cells, lymphocytes, and monocytes). The number of apoptotic cells reached 50-60%, and that of necrotic cells reached 7-10% (Figure 2P,Q). For the first 7 days after ELR, the _control_ group showed the survival rate almost equal to 50% as estimated using the log-rank test with \(p<0.05\). No additional mortality occurred  
Figure 2: **Histological slides of liver from rats before ELR and 48 h after. ****(A,B)—** intact liver at Day 0; (**D,E)—_control_ group; (**G,H**)—**group of _iHSC_; (**J,K**)—group of _aHSC_; (**M,N**)—**group of _total RNA_. The second row shows pseudostaining of the corresponding images from the first row (hepatocyte nuclei are blue, cytoplasm is green, empty spaces are red). Yellow arrows indicate mitotic hepatocytes. H&E staining, \(\times 200\). Scale bar 100 \(\upmu\)m. **Caspase-9 expression on histological slides of liver from rats 48 h after ELR. ****(C)—**intact liver at Day 0; (**F**)—_control_ group; (**I**)—group of _iHSC_; (**L**)—group of _aHSC_; (**O**)—**group of _total RNA_. Arrows indicate cells during mitosis. Although many of them are caspase-9-positive (i.e., in reversible apoptosis), the proliferation is evident. Anti-caspase-9 immunohistochemical staining with additional Mayer’s hematoxylin staining, \(\times 200\). Scale bar 100 \(\upmu\)m. **Cell cultures. ****(P)—**culture of cells from the bone marrow of rats, 7 days of cultivation, \(\times 100\); (**Q**)—non-attached HSCs washed from the surface of cultivated cells, \(\times 200\), phase contrast. in the _control_ group after Day 7. Groups of _iHSC_, _aHSC_, and _total RNA_ had no fatal outcomes during the entire study period (Figure 3A). The high mortality after surgery in the _control_ group is consistent with acute liver failure confirmed by an increase on Day 2 compared to Day 0 in AST (572 \(\pm\) 42.2 u/L to 71 \(\pm\) 14.8 u/L), ALT (315 \(\pm\) 3.7 u/L to 35 \(\pm\) 2.3 u/L), ALP (1102 \(\pm\) 37.2 u/L to 382 \(\pm\) 29.8 u/L), and total bilirubin (2 \(\pm\) 1.8 g/L to 10 \(\pm\) 2.1 g/L with the increase continuing up to Day 12) levels (markers of cell lysis) in the blood of the rats (Figure 4A-E). The _iHSC_, _aHSC_, and _total RNA_ groups exhibited a higher rate of biochemical recovery than the _control_ group, as shown by repeated t test comparisons with \(p\) < 0.05. In the _iHSC_, _aHSC_, and _total RNA_ groups, cell lysis markers also increased to a lesser extent during the first two days but recovered completely by Day 12-14. Thus, the AST levels on Day 2 were the least for the _total RNA_ group (330 \(\pm\) 29.7 u/L), ALT levels were the least for the _total RNA_ group (81 \(\pm\) 6.4 g/L), ALP levels were the least for the _aHSCs_ group (892 \(\pm\) 32.4 u/L), and total bilirubin was the least for the _total RNA_ (6 \(\pm\) 1.2 g/L) group. Figure 3: **Dynamics of animal survival, Ki-67 expression, and rat liver mass. ****(A)**—survival at percents after 75% ELR in rats (Kaplan–Meier). Groups of _iHSC_, _aHSC_, and _total RNA_ increased the survival rate after ELR (_p_ = 0.037, log-rank test); (**B**)—timepoints at dynamic logarithmic function of Ki-67 expression; *—the difference is significant compared with the _control_ group; \(p\) < 0.05; (**C**)—recovery of rat liver mass in grams after ELR. Values are given as means \(\pm\) SDs. Albumin levels in the _control_ group decreased with a peak by Day 2 (19.3 \(\pm\) 1.73 g/L), then gradually increased almost up to the Day 0 values (98.0 \(\pm\) 2.01 g/L) before the surgery on Day 14 (52.1 \(\pm\) 1.54 g/L). The albumin levels in the _iHSC_ (88.2 \(\pm\) 1.94 g/L), _aHSC_ (90.3 \(\pm\) 1.23 g/L), and _total RNA_ groups (90.5 \(\pm\) 0.94 g/L) almost recovered by the end of the study. Studying the histological specimens, we revealed no signs of bleeding, leukocyte infiltration, necrosis, or hemosiderin accumulation in the liver.