## 4 Discussion
\begin{table}
\begin{tabular}{c c c c} \hline \hline
**Strain** & **Species Identity** & **\% Identity \({}^{\star}\)** & **Accession Number** \\ \hline P24-1 & _Lactobacillus plantarum_ ATCC 14917 & 100 & KT025937.1 \\  & _Lactobacillus plantarum_ JCM 1149 & 100 & NR_115605.1 \\ P24-2 & _Lactobacillus pentosus_ 124-2 & 99.9 & NR_029133.1 \\  & _Lactobacillus plantarum_ JCM 1149 & 99.8 & NR_115605.1 \\ P24-3 & _Lactobacillus plantarum_ NBRC 15891 & 99.8 & NR_113338.1 \\  & _Lactobacillus plantarum_ JCM 1149 & 100 & NR_115605.1 \\ P24-4 & _Lactobacillus plantarum_ NBRC 15891 & 99.8 & NR_113338.1 \\  & _Lactobacillus plantarum_ ATCC 14917 & 100 & KT025937.1 \\ P24-5 & _Lactobacillus plantarum_ JCM 1149 & 100 & NR_115605.1 \\  & _Lactobacillus plantarum_ ATCC 14917 & 100 & KT025937.1 \\ P24-6 & _Lactobacillus plantarum_ JCM 1149 & 100 & NR_115605.1 \\  & _Lactobacillus plantarum_ CIP 103151 & 100 & NR_104573.1 \\  & _Lactobacillus plantarum_ JCM 1149 & 100 & NR_117813.1 \\ P24-7 & _Lactobacillus brachy_ ATCC 14869 & 100 & NR_116238.1 \\  & _Lactobacillus vaginalensis_ THK-V8 & 97.8 & NR_109452.1 \\  & _Lactobacillus plantarum_ CIP 103151 & 100 & NR_104573.1 \\ A1-2 & _Lactobacillus plantarum_ JCM 1149 & 100 & NR_117813.1 \\  & _Pediococcus acidilactici_ DSM 20284 & 99.6 & NR_042057.1 \\  & _Pediococcus acidilactici_ NGRI 0510Q & 98.3 & NR_041640.1 \\ \hline \hline \end{tabular} \({}^{\star}\) Identity were determined through pairwise alignments using the European Molecular Biology Open Software Suite (EMBOSS) Water algorithms.  
\end{table} 
Table 4: Identification of lactic acid bacteria isolates by 165 rRNA sequences. Bile salts resistance may also be attributed to the enzyme responsible for bile salt deconjugation, a hydrolase present in some LAB. This enzyme is also associated with the reduction of serum cholesterol, and the toxicity and side effects of bile salts [8; 25; 29]. The results of our study indicate that 70% of the evaluated strains showed equal or greater resistance than _L. casei Shirota_ to the gastrointestinal conditions. The antibacterial activity of LAB could result from the production of a great variety of compounds, including sugar catabolites (e.g., lactic acid and acetic acid); oxygen catabolites (such as hydrogen peroxide); proteinaceous compounds (e.g., bacteriocins, other low-molecular-mass peptides, and antifungal peptides/proteins); fat and amino acid metabolites (e.g., fatty acids, phenyllactic acid, and OH-phenyllactic acid); and others such as reuterin and reutericyclin [38]. Antibacterial capacity can also differ under the same pH conditions (e.g., the stronger effect of acetic acid compared to lactic acid under similar pH values) [7]. [PERSON] et al. [7] found that 50% of the isolates from swine (251 strains) inhibited the growth of _E. coli_ O55. Meanwhile, [PERSON] et al. [8] described that 45% of _L. fermentum_ strains inhibited _E. coli_ O157 882364, and all the strains had antimicrobial activity against _S. aureus_, revealing that even if the strains belong to the same species, their antibacterial effect can be different. Inhibition of Gram-negative bacteria could result from the production of lactic acid and/or other organic acids that can penetrate the cell membrane affecting its function, acidifying the cytoplasm, and inhibiting acid-sensitive enzymes [39]. The production of bacteriocins also contributes to the antibacterial activity against several Gram-positive and -negative pathogens, as well as the competition for nutrients, and hydrogen peroxide production [40; 41; 42]. In this study, 60% of the isolates showed antimicrobial activity against _E. coli_ and _S. aureus_. There is increasing evidence that highlights the efficacy of probiotics in the management of _Helicobacter pylori_ infection. Inhibition is associated with the production of antimicrobial compounds, such as lactic acid, and by competing for substrate and binding sites [43; 44]. Although ingestion of probiotics may reduce the density of infection, they are unable to completely eradicate _H.