## 4 Discussion
Oleic acid (C18:1 _n_-9), which is characterized by its cholesterol-lowering properties [72], was the most dominant acid among MUFA and was negatively affected by organic fertilization application, with the highest two-year average value (13.54% of DM) of its content observed in the unfertilized (control) plots. Hazardous erucic acid (C22:1) was the lowest MUFA in content and presented a similar response to that of total MUFA towards the examined factors' effects. The highest average 2-year values were observed under conventional tillage (2.17% DM) and untreated (2.23% DM). As for the above-mentioned results, similar behavior of MUFA and their consequents was also detected in previous research studying the influence of similar factors (minimum soil tillage and organic fertilization) on the fatty acid profile of amelina seed oil [50; 73; 74]. The current findings confirmed that amelina cake contains more MUFA than hempseed cake (10%) but less than soybean meal (52.4%) and rapeseed cake (48.7%) [70; 71]. Moreover, the erucic acid content in cold-pressed amelina cake seemed to be thirty-six times higher than that in rapeseed cake [71]. Camelina oilseed cake constitutes a major source of polyunsaturated fatty acids (PUFA), particularly linoleic acid (C18:2 _n_-6) and \(\alpha\)-lionlemic acid (C18:3 _n_-3), which are characterized as essential since they are essential for good health, and must be incorporated into the diet as they are not able to be produced by the body. Omega 6 (_n_-6) and omega 3 (_n_-3) are fatty acids of PUFA, and specifically in the present research, the _n_-6 and _n_-3 omega fatty acids in cold-pressed amelina cake were composed exclusively of linoleic and \(\alpha\)-lionlemic acid, respectively. According to the results of our study, the total PUFA and their constituents' contents were only affected by organic fertilization, and the highest average 2-year values of their contents were presented in oilseed cake obtained from plants fertilized with compost (52.68%, 22.49%, and 30.20% for total PUFA, linoleic acid, and \(\alpha\)-lionlemic acid, respectively). In the same manner, in the research study of [PERSON] et al. [74] evaluating the organic fertilization effect on amelina oil, the application of compost sludge seemed to significantly increase the proportion of linoleic acid, while there was a significant decrease in the proportion of erucic acid with respect to untreated plants. In addition, correlation analysis revealed highly significant correlations between the levels of linoleic and linolenic acids and between these fatty acids and seed cake extraction rate (Figure 3), which is in accordance with the relevant study of [PERSON] et al. [75] on the fatty acid profile of amelina seed oil. As for \(\alpha\)-linolenic acid, amelina cake presents a much higher content of this fatty acid in comparison with other feed ingredients, including soybean meal (7.2-8.6%), hempseed cake (15.9-24.7%), and rapeseed cake (10.6-13.1%), but lower than the relevant content recorded in linseed cake (51.5%) [52; 71; 76; 77]. As a result, it is important to remember that amelina is the second highest \(\alpha\)-linolenic acid-containing plant that is cultivated in the Northern Hemisphere and utilized as a feed ingredient [50; 69]. Furthermore, essential linoleic acid was found to be lower than in other feed ingredients, such as soybean meal (52.4-55.2%) and hempseed cake (52.5-59.5%), but lower than the relevant content recorded in linseed cake (14.60%) [52; 71; 77]. In response to the fatty acid ratios, the PUFA/SAFA were only affected by organic fertilization during the second year of the study, and the greatest value was found in compost plots, with the average values being 5.31, while the _n-6/n-_3 or linoleic/\(\alpha\)-linolenic ratio was not influenced by either examined factor, and its values ranged from 0.73 to 0.77%.