## 2 Results
### Identified Staphylococcus Comenes Impacting HMEC-1 Damages
\begin{table}
\begin{tabular}{c c c c} \hline \hline
**Locus** & **Group** & **Gene Name** & **\% EC Damage (Mean \(\pm\) SD)** \\ \hline JE2 & Wildtype & & \(46.19\) \({}^{a}\) \({}^{\pm}\) 2.97 & \(42.43\) \({}^{a}\) \({}^{\pm}\) 6.44 \\ \hline SAUSA300\_1197 & hypothetical & \(62.86\) \({}^{a}\) 5.67 & \(59.40\) \({}^{a}\) \({}^{\pm}\) 1.50 \\ SAUSA300\_1333 & hypothetical & hypothetical & \(62.17\) \({}^{a}\) 3.05 & \(66.92\) \({}^{\pm}\) 0.84 \\ SAUSA300\_1485 & EC damage \(\geq\) 60\% & hypothetical & \(61.86\) \({}^{a}\) 6.12 & \(61.75\) \({}^{a}\) \\ SAUSA300\_2221 & in 384-well plates & _moaD_ & \(61.64\) \({}^{a}\) 3.61 & \(59.90\) \({}^{a}\) \({}^{\pm}\) 1.08 \\ SAUSA300\_0206 & hypothetical & \(60.82\) \({}^{a}\) 6.24 & \(69.33\) \({}^{\pm}\) 0.48 \\ SAUSA300\_0335 & hypothetical & \(60.15\) \({}^{a}\) 8.31 & \(63.35\) \({}^{a}\) \({}^{\pm}\) 2.06 \\ \hline \hline \end{tabular}  
* ND: not determined.  
\end{table} 
Table 2: Mutants significantly increase HMEC-1 damage vs. JE2 WT strain (EC damage rate \(\geq\) 60%). \begin{table}
\begin{tabular}{c c c c} \hline \hline
**Locus** & **Gene Name** & **Description** & **\% EC Damage (Mean \(\pm\) SD)** \\ \hline SAUSA300\_0904 & _vbl_ & hypothetical protein & \(-1.32\) \({}^{a}\) \({}^{\pm}\) 9.61 \\ SAUSA300\_0423 & hypothetical & hypothetical protein & \(-2.20\) \({}^{a}\) \({}^{\pm}\) 9.08 \\ SAUSA300\_1422 & hypothetical & phiSLT ORF65-like protein & \(-2.77\) \({}^{a}\) \({}^{\pm}\) 6.35 \\ SAUSA300\_0068 & hypothetical & cadmium-exporting ATPase, truncation & \(-2.79\) \({}^{a}\) \({}^{\pm}\) 8.85 \\ SAUSA300\_1870 & hypothetical & hypothetical protein & \(-2.92\) \({}^{a}\) \({}^{\pm}\) 15.58 \\ SAUSA300\_1139 & _sucD_ & succinyl-CoA synthetase subunit alpha & \(-2.94\) \({}^{a}\) \({}^{\pm}\) 8.32 \\ SAUSA300\_0918 & _ugtP_ & diacylglycerol glucoseyltransferase & \(-3.09\) \({}^{a}\) \({}^{\pm}\) 8.63 \\ SAUSA300\_0597 & hypothetical & putative endonuclease III & \(-3.15\) \({}^{a}\) \({}^{\pm}\) 14.78 \\ SAUSA300\_0326 & hypothetical & hypothetical protein & \(-3.64\) \({}^{a}\) \({}^{\pm}\) 2.40 \\ SAUSA300\_0690 & _sucS_ & sensor histidine kinase SaeS & \(-4.88\) \({}^{a}\) \({}^{\pm}\) 14.01 \\ SAUSA300\_0560 & _vraB_ & acetyl-CoA C-acetyltransferase & \(-5.06\) \ \begin{table}
\begin{tabular}{c c c c} \hline \hline \multirow{2}{*}{**Categories**} & \multirow{2}{*}{**Sub-Groups**} & \multicolumn{2}{c}{**No. of Mutants with Decreased HMEC-1**} & \multicolumn{2}{c}{**No. of Mutants with Increased HMEC-1**} \\  & & **Damage** & **Damage** \\ \hline \multirow{8}{*}{Metabolism} & Carbohydrate metabolism & 53 & \\  & Amino acid metabolism & 33 & \\  & Metabolism of cofactors and vitamins & 11 & \\  & Lipid metabolism & 8 & 1 \\  & Nucleotide metabolism & 8 & \\  & Biosynthesis of other secondary metabolites & 7 & \\  & Energy metabolism & 7 & \\  & Metabolism of other amino acids & 3 & 1 \\  & Metabolism of terpenoids and polyketides & 3 & \\  & Glycan biosynthesis and metabolism & 2 & \\  & Xenobiotics biodegradation and metabolism & 1 & \\ \hline \multirow{8}{*}{Genetic information processing} & Homologous recombination & 4 & \\  & DNA replication & 2 & \\  & Mismatch repair & 2 & \\  & Protein export & 2 & \\  & Ribosome & 2 & \\  & Sulfur relay system & 2 & 1 \\  & RNA degradation & 1 & \\ \hline \multirow{4}{*}{Environmental information processing} & Two-component system & 13 & \\  & ABC transporters & 9 & \\  & Other & 3 & \\ \hline Cellular processes & Quorum sensing & 9 & \\ \hline Total & & 185 & 3 \\ \hline \hline \end{tabular}
\end{table} 
Table 4: Numbers of genes from different KEGG pathway categories.