## 3 Results
A significant difference in MDA concentration was observed between feeding groups A and B (\(p\) < 0.05). To clarify the relative importance of varying diet quality on the lipid composition and physiological condition of the meagre during marine cage farming, Principal Component Analysis (PCA) was performed. \begin{table}
\begin{tabular}{l c c c c c c} \hline  & \multicolumn{6}{c}{**Feed Mixtures**} \\ \cline{2-7}  & \multicolumn{2}{c}{**A**} & \multicolumn{2}{c}{**B**} & \multicolumn{2}{c}{**C**} & \multicolumn{1}{c}{**_p_ Level} \\ \hline
**Fatty Acid** & \(\mathbf{\overline{x}}\) & **SD** & \(\mathbf{\overline{x}}\) & **SD** & \(\mathbf{\overline{x}}\) & **SD** & \\ \hline C14:0 & 3.03 \({}^{\text{a}}\) & 0.68 & 2.80 \({}^{\text{b}}\) & 0.70 & 1.97 \({}^{\text{c}}\) & 0.413 & *** \\ C15:0 & 0.42 \({}^{\text{a}}\) & 0.11 & 0.34 \({}^{\text{b}}\) & 0.16 & 0.33 \({}^{\text{b}}\) & 0.07 & ** \\ C16:3 (n-3) & 1.00 \({}^{\text{ab}}\) & 0.39 & 1.21 \({}^{\text{a}}\) & 0.35 & 0.79 \({}^{\text{b}}\) & 0.20 & *** \\ C16:1 (n-7) & 4.10 \({}^{\text{a}}\) & 0.89 & 3.95 \({}^{\text{a}}\) & 0.93 & 3.24 \({}^{\text{b}}\) & 0.51 & ** \\ C16:0 & 17.27 \({}^{\text{a}}\) & 1.06 & 17.45 \({}^{\text{a}}\) & 1.19 & 16.03 \({}^{\text{b}}\) & 0.87 & *** \\ C17:1 (n-9) & 0.74 & 0.45 & 0.86 & 0.64 & 0.62 & 0.26 & ns \\ C18:3 (n-3) & 2.20 & 1.18 & 3.72 & 2.11 & 3.05 & 2.24 & ns \\ C18:2 (n-6) & 5.86 \({}^{\text{b}}\) & 2.18 & 7.76 \({}^{\text{b}}\) & 2.23 & 10.48 \({}^{\text{a}}\) & 2.62 & * \\ C18:1 & 17.97 \({}^{\text{ab}}\) & 3.00 & 17.43 \({}^{\text{b}}\) & 2.80 & 21.05 \({}^{\text{a}}\) & 4.77 & * \\ C18:0 & 6.50 \({}^{\text{b}}\) & 0.67 & 6.71 \({}^{\text{a}}\) & 0.59 & 6.66 \({}^{\text{b}}\) & 0.77 & * \\ C20:4 (n-6)\_ARA & 0.55 \({}^{\text{a}}\) & 0.07 & 0.53 \({}^{\text{a}}\) & 0.05 & 0.44 \({}^{\text{b}}\) & 0.05 & *** \\ C20:5 (n-3)\_EPA & 11.65 \({}^{\text{a}}\) & 1.56 & 11.21 \({}^{\text{a}}\) & 1.10 & 9.34 \({}^{\text{b}}\) & 1.10 & *** \\ C20:3 (n-6) & 1.40 & 0.29 & 1.38 & 0.32 & 1.34 & 0.18 & ns \\ C20:1 (n-7) & 1.56 \({}^{\text{a}}\) & 0.31 & 1.17 \({}^{\text{b}}\) & 0.21 & 1.26 \({}^{\text{b}}\) & 0.16 & *** \\ C20:0 & 0.27 \({}^{\text{ab}}\) & 0.11 & 0.23 \({}^{\text{b}}\) & 0.08 & 0.33 \({}^{\text{a}}\) & 0.06 & *** \\ C22:6 (n-3)\_DHA & 21.59 \({}^{\text{a}}\) & 3.46 & 19.69 \({}^{\text{a}}\) & 3.61 & 17.29 \({}^{\text{b}}\) & 3.55 & ** \\ C22:4 (n-6) & 1.92 \({}^{\text{b}}\) & 0.17 & 1.65 \({}^{\text{a}}\) & 0.23 & 1.83 \({}^{\text{b}}\) & 0.25 & * \\ C22:1 (n-9) & 1.98 \({}^{\text{a}}\) & 0.54 & 1.17 & 0.38 & 1.22 \({}^{\text{b}}\) & 0.29 & *** \\ C22:0 & 0.23 & 0.12 & 0.15 & 0.018 & 0.19 & 0.043 & ns \\ C24:1 & 0.68 \({}^{\text{a}}\) & 0.22 & 0.49 \({}^{\text{b}}\) & 0.13 & 0.46 \({}^{\text{b}}\) & 0.09 & *** \\ \hline SFA & 27.49 \({}^{\text{a}}\) & 1.25 & 27.61 \({}^{\text{a}}\) & 1.50 & 25.43 \({}^{\text{b}}\) & 1.35 & *** \\ UNISFA & 72.51 \({}^{\text{b}}\) & 1.25 & 72.39 \({}^{\text{b}}\) & 1.50 & 74.57 \({}^{\text{a}}\) & 1.35 & *** \\ MUFA & 26.90 & 4.19 & 25.76 & 3.71 & 27.74 & 4.47 & ns \\ PUFA & 45.62 & 4.44 & 46.63 & 4.65 & 46.83 & 5.13 & ns \\ n-6 & 9.19 \({}^{\text{b}}\) & 4.58 & 12.34 \({}^{\text{ab}}\) & 4.42 & 14.50 \({}^{\text{a}}\) & 7.16 & ** \\ n-3 & 35.48 \({}^{\text{a}}\) & 6.12 & 34.56 \({}^{\text{a}}\) & 6.28 & 30.65 \({}^{\text{b}}\) & 6.82 & * \\ n-3/n-6 & 3.86 \({}^{\text{a}}\) & 1.54 & 2.80 \({}^{\text{b}}\) & 1.13 & 2.11 \({}^{\text{c}}\) & 0.86 & ** \\ DHA/EPA & 1.96 & 0.40 & 1.70 & 0.28 & 1.83 & 0.22 & ns \\ \hline Total lipids & 16.81 & 7.84 & 13.04 & 5.78 & 11.18 & 3.59 & ns \\ \hline \end{tabular}
\end{table} 
Table 3: Fatty acid profile of muscle tissue of meagre _A. regius_ fed diets containing different levels of fish oil (% of total fatty acids). The relationship among feed quality (Prot/Fat), unsaturated FA content, oxidative stress (MDA) and physiological condition indices (Kffin, SGR) in meagre groups fed different diets, are shown on the principal component plot (Figure 4) where PC1 and PC2 explained 66.6% and 26.0% of total variance, respectively.