## 3 Results
### In Vitro Incubation and Degradability Measurements
\begin{tabular}{l c c c c c c c c c} \hline \hline \multirow{2}{*}{**Item**} & \multirow{2}{*}{**Temperature**} & \multicolumn{4}{c}{**Inoculant**} & \multicolumn{4}{c}{**Significance**} \\ \cline{3-10}  & & **Control** & **LF18** & **LP23** & & **I** & **T** & **I \(\times\) T** \\ \hline \multirow{3}{*}{DM (g kg\({}^{-1}\) FM)} & 20 \({}^{\circ}\)C & 371.1 & 362.7 & 357.9 & 1.88 & 0.218 & 0.729 & 0.524 \\  & 30 \({}^{\circ}\)C & 369.8 & 370.2 & 358.4 & & & & \\  & 40 \({}^{\circ}\)C & 364.9 & 357.9 & 364.6 & & & & \\ \hline \multirow{3}{*}{WSC (g kg\({}^{-1}\) DM)} & 20 \({}^{\circ}\)C & 39.0 \({}^{\mathrm{A}}\) & 41.2 \({}^{\mathrm{A}}\) & 42.8 \({}^{\mathrm{A}}\) & 0.42 & 0.010 & \textless{}0.001 & 0.053 \\  & 30 \({}^{\circ}\)C & 32.0 \({}^{\mathrm{B}}\) & 35.4 \({}^{\mathrm{a}\mathrm{B}}\) & 38.4 \({}^{\mathrm{a}\mathrm{A}}\) & & & & \\  & 40 \({}^{\circ}\)C & 32.4 \({}^{\mathrm{B}}\) & 35.7 \({}^{\mathrm{B}}\) & 31.7 \({}^{\mathrm{B}}\) & & & & \\ \hline \multirow{3}{*}{CP (g kg\({}^{-1}\) DM)} & 20 \({}^{\circ}\)C & 109.8 \({}^{\mathrm{A}}\) & 109.9 \({}^{\mathrm{A}}\) & 109.7 \({}^{\mathrm{A}}\) & 0.34 & 0.262 & \textless{}0.001 & 0.195 \\  & 30 \({}^{\circ}\)C & 109.4 \({}^{\mathrm{A}}\) & 105.6 \({}^{\mathrm{B}}\) & 105.9 \({}^{\mathrm{B}}\) & & & & \\  & 40 \({}^{\circ}\)C & 104.7 \({}^{\mathrm{B}}\) & 105.6 \({}^{\mathrm{B}}\) & 104.1 \({}^{\mathrm{B}}\) & & & & \\ \hline \multirow{3}{*}{aNDF (g kg\({}^{-1}\) DM)} & 20 \({}^{\circ}\)C & 572.9 \({}^{\mathrm{B}}\) & 564.6 & 557.4 \({}^{\mathrm{C}}\) & 1.40 & 0.014 & \textless{}0.001 & 0.273 \\  & 30 \({}^{\circ}\)C & 585.2 \({}^{\mathrm{A}}\) & 569.3 \({}^{\mathrm{b}}\) & 571.5 \({}^{\mathrm{b}\mathrm{B}}\) & & & & \\  & 40 \({}^{\circ}\)C & 585.2 \({}^{\mathrm{A}}\) & 579.2 & 585.5 \({}^{\mathrm{A}}\) & & & & \\ \hline \multirow{3}{*}{ADF (g kg\({}^{-1}\) DM)} & 20 \({}^{\circ}\)C & 314.1 & 313.0 & 308.0 \({}^{\mathrm{B}}\) & 1.59 & 0.754 & 0.023 & 0.293 \\  & 30 \({}^{\circ}\)C & 319.1 & 315.5 & 314.0 \({}^{\mathrm{B}}\) & & & & \\  & 40 \({}^{\circ}\)C & 315.4 & 328.0 & 327.1 \({}^{\mathrm{A}}\) & & & & \\ \hline \multirow{3}{*}{HC (g kg\({}^{-1}\) DM)} & 20 \({}^{\circ}\)C & 258.8 & 251.6 & 249.5 & 1.95 & 0.040 & 0.347 & 0.889 \\  & 30 \({}^{\circ}\)C & 266.1 & 253.8 & 257.5 & & & & \\  & 40 \({}^{\circ}\)C & 269.8 & 251.2 & 258.4 & & & & \\ \hline \multirow{3}{*}{CE (g kg\({}^{-1}\) DM)} & 20 \({}^{\circ}\)C & 293.5 & 291.3 & 288.4 & 1.78 & 0.556 & 0.379 & 0.232 \\  & 30 \({}^{\circ}\)C & 296.2 & 294.4 & 292.3 & & & & \\  & 40 \({}^{\circ}\)C & 277.0 & 295.4 & 291.8 & & & & \\ \hline \multirow{3}{*}{ADL (g kg\({}^{-1}\) DM)} & 20 \({}^{\circ}\)C & 20.6 \({}^{\mathrm{B}}\) & 21.7 \({}^{\mathrm{B}}\) & 19.5 \({}^{\mathrm{C}}\) & 0.58 & 0.317 & \textless{}0.001 & 0.396 \\  & 30 \({}^{\circ}\)C & 26.8 \({}^{\mathrm{B}}\) & 25.2 \({}^{\mathrm{B}}\) & 25.9 \({}^{\mathrm{B}}\) & & & \\  & 40 \({}^{\circ}\)C & 38.4 \({}^{\mathrm{A}}\) & 32.6 \({}^{\mathrm{A}}\) & 35.3 \({}^{\mathrm{A}}\) & & & & \\ \hline \multirow{3}{*}{Ash (g kg\({}^{-1}\) DM)} & 20 \({}^{\circ}\)C & 90.0 & 93.3 \({}^{\mathrm{A}}\) & 91.6 & 0.33 & 0.034 & 0.438 & 0.564 \\  & 30 \({}^{\circ}\)C & 89.3 & 90.6 \({}^{\mathrm{B}}\) & 92.2 & & & & \\  & 40 \({}^{\circ}\)C & 89.7 & 91.1 \({}^{\mathrm{B}}\) & 91.2 & & & & \\ \hline \multirow{3}{*}{Weight loss (g kg\({}^{-1}\) FM)} & 20 \({}^{\circ}\)C & 28.9 \({}^{\mathrm{a}}\) & 13.8 \({}^{\mathrm{b}}\) & 13.6 \({}^{\mathrm{b}}\) & 1.05 & 0.005 & 0.309 & 0.106 \\  & 30 \({}^{\circ}\)C & 24.6 & 14.6 & 13.1 & & & & \\  & 40 \({}^{\circ}\)C & 14.4 & 15.5 & 14.5 & & & & \\ \hline \multirow{3}{*}{DM loss (g kg\({}^{-1}\) DM)} & 20 \({}^{\circ}\)C & 40.7 & 47.9 & 60.3 & 4.73 & 0.538 & 0.788 & 0.561 \\  & 30 \({}^{\circ}\)C & 40.1 & 29.1 & 58.5 & & & & \\ \cline{1-1}  & 40 \({}^{\circ}\)C & 42.6 & 62.0 & 43.7 & & & & \\ \hline \hline \end{tabular}