# Isolation and Characterization of a Novel Cyanophage Encoding Multiple Auxiliary Metabolic Genes
## 4 Conclusions  
In this study, a novel cyanophage, S-SZBM1, infecting _Synechococcus_ CB0101 was isolated and characterized based on its physiological characteristics, taxonomy, and genomic and proteomic features. As a myovirus, cyanophage S-SZBM1 does not have a broad host range as expected and only shows lysis ability to its host strain. Surprisingly, up to 25 AMGs are encoded by cyanophage S-SZBM1, which potentially re-program the phage-host interactions. These AMGs are mainly involved in photosynthesis, carbon metabolism, nucleotide synthesis and metabolism, cell protection, and other cell metabolism. Taxonomy analysis showed that cyanophage S-SZBM1 belongs to a new genus under the family _Kyanoviridae_. According to the assembly pathways in bacteriophage T4, the structural assembly processes during infection were also speculated based on the identified structural genes of cyanophage S-SZBM1. Taken together, this study represents the structural and genomic characterization of a newly isolated and distinct cyanophage.  
The following supporting information can be downloaded at: [[https://www.mdpi.com/article/10.3390/v14050887/s1](https://www.mdpi.com/article/10.3390/v14050887/s1)]([https://www.mdpi.com/article/10.3390/v14050887/s1](https://www.mdpi.com/article/10.3390/v14050887/s1)), Table S1: Genome annotations of cyanophage S-SZBM1; Table S2: T4-like core genes in cyanophage S-SZBM1 genome; Table S3: tRNA genes predicted in the genome of cyanophage S-SZBM1; Table S4: homologs of cyanophage S-SZBM1 predicted in the non-redundant database based on the complete nucleotide sequence.  
**Author Contributions:** [PERSON], [PERSON] and [PERSON] supervised the project and revised the manuscript; [PERSON] and [PERSON] performed the experiments; [PERSON], [PERSON], [PERSON], [PERSON], [PERSON] and [PERSON] analyzed the data and wrote the manuscript. All authors have read and agreed to the published version of the manuscript.  
**Funding:** This research was funded by Scientific and Technical Innovation Council of Shenzhen (JCYJ20210324093412035), Guangdong Natural Science Foundation (2020A1515011117), National Natural Science Foundation of China (42106194), China Postdoctoral Science Foundation (2019 M662237), and Science and Technology Program of Guangzhou, China (201904020029).  
**Institutional Review Board Statement:** Not applicable.  
**Informed Consent Statement:** Not applicable.  
**Data Availability Statement:** The complete genome sequence of cyanophage S-SZBM1 was submitted to the GenBank database under accession number OL473597.  
**Acknowledgments:** We thank [PERSON], [PERSON], [PERSON], and [PERSON] of the State Key Laboratory of Marine Environmental Science for their useful suggestions and help.  
\begin{table}
\begin{tabular}{c c c c c c c c c} \hline \hline
**Locus Tag** & **Putative Function** & **Mass** & **aa** & **Petides** & **Coverage** & **Score** & **\(p\)-Value** & **em PAI** \\ \hline S-SZBM1\_207 & Structural protein & 58.61 & 532 & 1 & 2.6\% & 245 & \(3.16\times 10^{-25}\) & 0.12 \\ \hline S-SZBM1\_51 & Hypothetical protein & 30.51 & 287 & 1 & 4.9\% & 120 & \(1.00\times 10^{-12}\) & 0.11 \\ \hline S-SZBM1\_198 & Neck protein & 32.36 & 291 & 1 & 8.6\% & 74 & \(3.98\times 10^{-8}\) & 0.10 \\ \hline \hline \end{tabular}
\end{table}
Table 1: _Cont._