# Reactive Polymer Composite Microparticles Based on Glycidyl Metacrylate and Magnetic Nanoparticles
## 2 Experimental Part
### General Procedure of Preparation of Glycidyl-Functionalized Polymer Composite Microparticles  
A three-neck flask with a capacity of 250 mL equipped with a mechanical stirrer, a thermometer, dropping funnel, an argon supply system (an argon balloon), and a heating mantle were used to react 2.35 g (0.008 mol) FeSO4\(\cdot\)7H\({}_{2}\)O and 2.45 g (0.015 mol) FeCl\({}_{3}\) dissolved in 100 mL of deionized water with 25 mL of 28 wt.% ammonia solution. The ammonia solution was added dropwise under an argon atmosphere at room temperature. The reaction mixture was stirred intensively during this operation. Then, the mixture was heated to 80 \({}^{\circ}\)C, and 1 mL of oleic acid was added very slowly dropwise. The mixing was continued at 80 \({}^{\circ}\)C for 1 h upon allowing the reaction mixture to cool to ambient temperature. Then, black precipitate was extracted twice with toluene (2 \(\times\) 50 mL) to transfer the hydrophobic Fe\({}_{3}\)O\({}_{4}\) particles provided from water to toluene. The resulting toluene dispersion was dried by azeotropic distillation using a Dean-Stark apparatus and then diluted with dry toluene to obtain the final concentration of 10 mg of Fe\({}_{3}\)O\({}_{4}\) in 1 mL of toluene. The final black dispersion was applied for the synthesis of polymer particles. To perform the polymerization, a cylindrical-shaped 100 mL reactor equipped with a heating coat, a reflux condenser, and a mechanical stirrer was used. Monomers (20 mol% GMA, 77 mol% S and 3 mol% DVB), an initiator (benzoyl peroxide), and the dispersion of Fe\({}_{3}\)O\({}_{4}\)@OA in toluene were mixed, diluted with toluene, and then sonicated using an ultrasonic bath for 15 min. The organic phase prepared in this manner was then dispersed slowly in a poly(vinyl alcohol) water solution (1% PVA, 18-88, Fluka). A 100 mL cylindrical glass reactor equipped with a heating coat, a mechanical stirrer, and a reflux condenser was used to perform the polymerization. The stirring rate was adjusted to 350 rpm. The mixture reacted under an argon atmosphere at 70 \({}^{\circ}\)C for 2 h and at 80 \({}^{\circ}\)C for 6 h. After the reaction, the mixture was cooled to ambient temperature, and it was poured into a glass beaker with water. The polymer particles were then washed several times with water until a clear filtrate was obtained, and then filtered on a Buchner funnel. The particles were then transferred to a Soxhlet apparatus, where extraction with acetone was carried out for 6 h. Subsequently, the microbeads were resuspended in methanol, filtered, and dried at 40 \({}^{\circ}\)C under reduced pressure.  
Detailed synthesis conditions and characteristics of polymer products are presented in Table 1.  
\begin{table}
\begin{tabular}{c c c c c c c c} \hline \multirow{2}{*}{**Entry**} & \multirow{2}{*}{**Abbr.**} & \multirow{2}{*}{\
\begin{tabular}{c} **Fe\({}_{3}\)O\({}_{4}\)** \\ **wt.\%** \\ \end{tabular} } & \multirow{2}{*}{
\begin{tabular}{c} **Average Size**, **\(\mu\)m** \\ \end{tabular} } & \multirow{2}{*}{**Diameter**} & \multirow{2}{*}{**Fraction, \%**} & \multicolumn{3}{c}{**Fe Content**} \\ \cline{6-9}  & & & & & & **Calculated, wt.\%** & **Defermed,** **wt.\%** & **Modification Degree, \%** \\ \hline
1 & **1a** & - & 211 & 100–448 & 100 & - & - & - \\
2 & **1b** & 0.05 & 272 & 142–632 & 100 & 0.036 & 0.013 & 36.0 \\
3 & **1c** & 0.10 & 328 & 159–710 & 100 & - & - & - \\
4 & **1d** & 0.25 & 386 & 159–1002 & 95.1 & 0.181 & 0.048 & 26.5 \\
5 & **1e** & 0.50 & 256 & 112–633 & 95.7 & 0.361 & 0.068 & 18.8 \\
6 & **1f** & 0.75 & 298 & 142–633 & 100 & 0.542 & 0.095 & 17.5 \\
7 \({}^{*}\) & **1g** & 0.50 & 247 & 100–564 & 100 & - & - & - \\
8 \({}^{**}\) & **1h** & 0.50 & 173 & 50—448 & 88.8 & - & - & - \\ \hline \end{tabular}
\end{table}
Table 1: Polymerization conditions and polymer particle characteristics.