## 1 Introduction  
The increasing mortality rate of infectious diseases is one the most challenging public health problems faced by different countries worldwide. This compromises and poses a threat to human health. Numerous synthetic antibiotic agents have always been used for the management of infectious diseases. Looking at the figures from 2000 to 2015, the statistics confirm that global antibiotics consumption levels have reached 65%, including the use of strong antibiotic agents like colistin and carbapenem [1]. Unfortunately, the wide use of antibiotics has increased the development of bacterial resistant strains to antibiotics [2], which has resulted in a reduction in the effectiveness of some of the antibacterial agents, leading to high mortality rates. Antibiotic resistance is considered one of the world's most urgent public health problems [3, 4]. A literature review has provided guidelines to minimize the increase of antibiotic resistance in the management of infections. Antibiotic resistance can be reduced by making an appropriate, timely diagnosis before doing any treatment planning; proper prescription and use of antibiotics by physicians as well as patients; effective implementation of strategies to prevent the transmission of infectious diseases; and discovering new antibacterial agents might help to control microbial drug resistance [5]. Of these, invention of new antimicrobial agents has been given more attention compared to other strategies [6]. Medicinal plants have been used for many years in the treatment of a vast number of human diseases by the community, specifically in traditional medicine. They are considered the main source of new, natural, and safe drugs to be utilized in managing diseases as an effective and harmless alternative medicine [7; 8], because they are not expensive and pose minimum side effects to humans. According to a report published in 2002 by the World Health Organization (WHO) in Geneva, medicinal plants have significant value and could be considered as the best source of complementary and alternative natural medicines [9]. Garlic, scientifically known as _Allium sativum_, is one of the oldest plants used as a spice in food and also used as a medicine because of its many benefits to human health and wellbeing [10; 11]. Findings have shown that garlic can be used in the management of various diseases such as cardiovascular disease and hyperglycemia. Additionallygarlic has been approved to reduce the risk of cancer, boosting the immune system and protecting against inflammation as well as infectious diseases [12; 13; 14]. Results from different studies have shown that garlic extracts have the capacity to inhibit the growth of some pathogenic microorganisms [15; 16]. Their antimicrobial activity has been linked to the presence of sulphur compounds [17], specifically, allicin which is the compound produced by the alliin lyase enzyme, after crushing or bruising a garlic bulb [12]. Many medical bacteria, including gram-positive and gram-negative strains, are sensitive to garlic extracts [18; 19], indicating that that garlic has a reliable broad-spectrum of activity related to its chemical composition [20]. However, the amounts and types of antimicrobial substances extracted depend on diverse extraction methods. Ultrasound-assisted extraction is considered as one of the best green extraction methods for extracting bioactive compounds from various spices, including garlic. The advantages include low-temperature extraction, easy operation, less cost, time, and energy requirement, and reduced use of toxic chemicals [21; 22]. Several studies have conducted ultrasonic-assisted extraction of bioactive antimicrobial substances from garlic such as allicin, essential oil, flavonoids, polyphenols, and sulfur compounds [23; 24; 25; 26; 27]. However, despite numerous studies done on garlic extract particles against bacteria, the antibacterial activity of probe ultrasonicated garlic extract against _Staphylococcus aureus_ sub.