# Physiological and Biochemical Variations in Celery by Imidacloprid and Fenpyroximate
## 3 Results
### Soluble Protein Accumulation  
The effect of spraying celery plants grown under two pesticides (IMI and FEN) with three concentrations (0, 1 F, and 10 F) after 2 h, 1, 3, 5, 8, 10, 14, 21, and 28 days on soluble protein (SP) content was examined. The concentration of SP in stem and leaf was measured by a microplate reader at 595 nm (Figure 2). After 2 h of spraying celery with IMI and FEN (for tissue stem), the SP in the T\({}_{2}\) (Figure 2A) and T\({}_{3}\) (Figure 2B) treatments changed dramatically when compared to the non-pesticded treatment. Furthermore, the SP concentration in stem dropped by 20.05% (T\({}_{1}\)), 31.63% (T\({}_{2}\)), 4.61% (T\({}_{3}\)) and 26.20% (T\({}_{4}\)) after \begin{table}
\begin{tabular}{c c c c c} \hline \hline \multirow{2}{*}{**Pesticides**} & \multirow{2}{*}{**Tissues**} & **Dosage** & **Residual** & **Correlation** & **Half-Life** \\  & & **(g a.i.h.m\({}^{-2}\))** & **Kinetics** & **Coefficient (R)** & **(T\({}_{1/2}\), Days)** \\ \hline \multirow{3}{*}{IMI} & \multirow{2}{*}{stem} & 30 & C\({}_{\text{t}}\) = 0.1864 e\({}^{-0.1898\text{t}}\) & 0.8154 & 3.65 \\  & & 300 & C\({}_{\text{t}}\) = 2.3015 e\({}^{-0.2400\text{t}}\) & 0.9182 & 2.89 \\ \cline{2-5}  & \multirow{2}{*}{leaf} & 30 & C\({}_{\text{t}}\) = 0.7349 e\({}^{-0.1259\text{t}}\) & 0.8478 & 5.51 \\  & & 300 & C\({}_{\text{t}}\) = 9.8642 e\({}^{-0.1707\text{t}}\) & 0.8782 & 4.06 \\ \hline \multirow{3}{*}{FEN} & \multirow{2}{*}{stem} & 37.5 & C\({}_{\text{t}}\) = 0.9038 e\({}^{-0.1671\text{t}}\) & 0.9914 & 4.15 \\  & & 375 & C\({}_{\text{t}}\) = 7.0234 e\({}^{-0.1748\text{t}}\) & 0.9773 & 3.97 \\ \cline{1-1} \cline{2-5}  & \multirow{2}{*}{leaf} & 37.5 & C\({}_{\text{t}}\) = 1.4326 e\({}^{-0.1441\text{t}}\) & 0.9466 & 4.81 \\ \cline{1-1}  & & 375 & C\({}_{\text{t}}\) = 11.0467 e\({}^{-0.1411\text{t}}\) & 0.9547 & 4.91 \\ \hline \hline \end{tabular}
\end{table} 
Table 1: T\({}_{1/2}\) and residual kinetics of IMI and FEN in celery under 1 F and 10 F after application. Figure 1: The residues of Imidadprid (IMI) and fenpyroximate (FEN) in stem and leaf after spraying IMI and FEN at the 1 F (**A**) and 10 F (**B**), respectively. one day of application, and there were significant differences between CK and treatments (T\({}_{1}\), T\({}_{2}\) and T\({}_{4}\)), which indicated that the celery itself has made a stress response due to the residues. Additionally, following a 3-day application of pesticides, SP concentration showed a considerable increase. Additionally, a similar phenomenon was observed after a 5-day application. After an 8-day treatment, the content of SP was found to be significantly lower in T\({}_{1}\) and T\({}_{3}\). Under treatments with CK, there were no significant differences after 10 and 14 days of application. On the 28 th day, a significant increase in T\({}_{1}\) and T\({}_{4}\) was seen. Positive feedback was seen at an intermediate concentration of 1 F and a ceiling concentration of 10 F, implying that the disparate stress reaction occurred in the tissue stem after spraying celery with varying concentrations of IMI and FEN. In contrast, the levels of SP in tissue leaf increased significantly after 2 h application in all treatments (T\({}_{1}\), T\({}_{2}\), T\({}_{3}\), and T\({}_{4}\)) (Figure 2C,D), with the SP amount increasing by 34.88% (T\({}_{1}\)), 78.94% (T\({}_{2}\)), 15.85% (T\({}_{3}\)) and 76.26% (T\({}_{4}\)) in leaf after 2 h when compared to the non-pesticded condition. The leaf tissue's reaction to pesticide was speedier than the stem tissue's, which could be owing to the leaf tissue's higher residue. A notable difference was seen at the same dose with two pesticides, namely, a significant increase and a decrease in T\({}_{1}\) and T\({}_{3}\) on one day, respectively, implying that celery is more adaptable to pesticide IMI when compared to FEN, a similar conclusion could be found at the results of 5, 14 and 21-day application. Furthermore, the levels of SP in treatment groups IMI and FEN on 5-day application were considerably increased and decreased, respectively.