# Anthocyanins, Phenolic Compounds, and Antioxidants from Extractions of Six _Eucalyptus_ Species
## 3 Results
### Extract Composition  
The levels of total phenolics and anthocyanins obtained varied among species (Tables 4 and 5). Our results show the highest phenolic content was obtained 124.17 mg GAE/g of extract for _E. nitens_ with methanol. On the other hand, the lowest phenolic content obtained was 1.57 mg GAE/g of extract for _E. oblicua_ with hexane.  
Phenolic content was higher for all five species of _Eucalyptus_ when using the polar solvents methanol (20.10-124.17 mg GAE/g of extract) and ethanol (17.72-112.57 mg GAE/g of extract), than when using non-polar solvents such as hexane (1.57-8.06 mg GAE/g of extract) (Table 4). The anthocyanin content varied greatly depending on the species and solvent (Table 5).  
Figure 2: Extraction yields obtained from leaves of six _Eucalyptus_ species mixed with solvents of different polarity, using a Soxllet extractor.  
\begin{table}
\begin{tabular}{c c c c c c c c} \hline \hline
**Solvent** & **Method.** & _E. globulus_ & _E. oblicua_ & _E. pavafflora_ &
\begin{tabular}{c} _E. canaldu-_ \\ _lensis_ \\ \end{tabular} & _E. vininalis_ & _E. nitens_ \\ \hline \multirow{3}{*}{DCH} & SLE 20 & 10.23 & 8.72 & 13.44 & 10.12 & 15.28 & 11.10 \\ \cline{2-8}  & SLE 35 & 15.33 & 9.21 & 14.21 & 11.49 & 20.62 & 17.32 \\ \hline \multirow{3}{*}{MTH} & SLE 20 & 23.11 & 20.54 & 19.76 & 22.14 & 24.71 & 23.18 \\ \cline{2-8}  & SLE 35 & 30.82 & 23.66 & 28.22 & 34.11 & 36.29 & 32.17 \\ \cline{2-8}  & SLE 50 & 36.12 & 29.74 & 32.14 & 40.87 & 42.11 & 40.26 \\ \hline \multirow{3}{*}{ETH} & SLE 20 & 15.17 & 8.52 & 20.08 & 12.44 & 18.33 & 14.01 \\ \cline{2-8}  & SLE 35 & 19.33 & 9.44 & 26.76 & 28.19 & 29.10 & 30.45 \\ \cline{2-8}  & SLE 50 & 32.15 & 9.78 & 31.11 & 35.87 & 34.52 & 30.74 \\ \hline \multirow{3}{*}{HEX} & SLE 20 & 5.77 & 3.22 & 6.54 & 7.08 & 9.21 & 6.24 \\ \cline{2-8}  & SLE 35 & 6.75 & 4.18 & 7.26 & 10.05 & 14.70 & 10.83 \\ \cline{2-8}  & SLE 50 & 11.90 & 4.97 & 8.94 & 13.27 & 18.43 & 18.22 \\ \hline \multirow{3}{*}{AC} & SLE 20 & 11.33 & 12.07 & 10.27 & 14.00 & 13.57 & 9.08 \\ \cline{2-8}  & SLE 35 & 15.80 & 14.66 & 11.35 & 14.35 & 15.21 & 10.74 \\ \cline{2-8}  & SLE50 & 24.51 & 17.14 & 15.14 & 20.17 & 22.14 & 19.72 \\ \hline \multirow{3}{*}{W} & SLE 50 & 20.71 & 17.21 & 16.16 & 14.21 & 15.14 & 13.11 \\ \cline{2-8}  & HD 50 & 12.11 & 11.03 & 10.44 & 13.18 & 15.21 & 9.07 \\ \hline \hline \end{tabular}  
* Water (W), acetone (AC), ethanol (ETH), methanol (MTH), hexane (HEX), and dichloromethane (DCH).  
\end{table}
Table 3: Total extraction yield results for SLE and HD methods (%).  
Two different antioxidant capacity assays (ABTS, CUPRAC) were evaluated for each of the study species. The ABTS assay was based on the ability of the antioxidants to remove ABTS-+, while CUPRAC measured the ability of the antioxidants to reduce Cu(II) to Cu(I). Knowledge of antioxidant activity could be useful for the standardisation of Eucalyptusptus raw materials. The highest antioxidant activity by ABTS and CUPRAC methods was for acetone extracts (10.72 and 3.44 mmol g\({}^{-1}\), respectively for _Eucalyptusptus globules_) (Table 6).