# Influence of _Eisenia fetida_ on the Nematode Populations during Vermicomposting Process
## 3 Results and Discussion
\begin{table}
\begin{tabular}{c c} \hline \hline
**Number of Species in Genus. N = Number of Species** & **Species with GenBank Records.** \\  & **n = Number of Records (Records Include at Least 18S and 28S rRNA Sequences)** \\ \hline \multicolumn{3}{c}{_P. boliviae, n = 28_} \\ \hline \multicolumn{3}{c}{_P. barbonicus, n = 25_} \\ \hline \multicolumn{3}{c}{_P. brevicauda, n = 4_} \\ \hline \multicolumn{3}{c}{_P. buccientus, n = 28_} \\ \hline \multicolumn{3}{c}{_P. bulgarcius, n = 28_} \\ \hline \multicolumn{3}{c}{_P. chinensis_\({}^{*}\)} \\ \hline \multicolumn{3}{c}{_P. clawus, n = 28_} \\ \hline \multicolumn{3}{c}{_P. degavai, n = 1 18 SRNA, n = 39 COI_} \\ \hline \multicolumn{3}{c}{_P. dorci, n = 2, 18 SRNA only_} \\ \hline \multicolumn{3}{c}{_P. elegans, n = 6_} \\ \hline \multicolumn{3}{c}{_P. entomophagus_\({}^{*}\)} \\ \hline \multicolumn{3}{c}{_P. entomophilus, n = 2, 18 SRNA only_} \\ \hline \multicolumn{3}{c}{_P. cospectralus_\({}^{*}\)} \\ \hline \multicolumn{3}{c}{_P. fissidentatus_\({}^{*}\)} \\ \hline \multicolumn{3}{c}{_P. fukushima, n = 28_} \\ \hline \multicolumn{3}{c}{_P. hongkongensis, n = 1, 18 SRNA only_} \\ \hline \multicolumn{3}{c}{_P. hoplostomus, n = 28_} \\ \hline \multicolumn{3}{c}{_P. japonicas_\({}^{*}\)} \\ \hline \multicolumn{3}{c}{_P. kurosawai, n = 1, 18S RNA only_} \\ \hline \multicolumn{3}{c}{_P. laevicollis, n = 4, 18S RNA only_} \\ \hline \multicolumn{3}{c}{_P. lheritieri, n = 61_} \\ \hline \multicolumn{3}{c}{_P. lucani, n = 3_} \\ \hline \multicolumn{3}{c}{_P. magnolia_\({}^{*}\)} \\ \hline \multicolumn{3}{c}{_P. mariannenea, n = 32_} \\ \hline \multicolumn{3}{c}{_P. maupsi, n = 54_} \\ \hline \multicolumn{3}{c}{_P. maxplanci_\({}^{*}\)} \\ \hline \multicolumn{3}{c}{_P. mayeri_\({}^{*}\)} \\ \hline \multicolumn{3}{c}{_P. musae_\({}^{*}\)} \\ \hline \multicolumn{3}{c}{_P. neolucani, n = 1, 18S RNA only_} \\ \hline \multicolumn{3}{c}{_P. mudus, n = 1, 18S RNA only_} \\ \hline \multicolumn{3}{c}{_P. oecultus, n = 1, 18S RNA only_} \\ \hline \multicolumn{3}{c}{_P. pacificus_\({}^{*}\)} \\ \hline \multicolumn{3}{c}{_P. ct. pacificus, n = 4_} \\ \hline \multicolumn{3}{c}{_P. paramundus_\({}^{*}\)} \\ \hline \multicolumn{3}{c}{_P. grasslandorum, n = 2, 18S RNA only_} \\ \hline \multicolumn{3}{c}{_P. pauli, n = 32_} \\ \hline \multicolumn{3}{c}{_P. paulseni, n = 1, 18S RNA only_} \\ \hline \multicolumn{3}{c}{_P. pseudaerivorus, n = 35_} \\ \hline \multicolumn{3}{c}{_P. purgamentorium_\({}^{*}\)} \\ \hline \end{tabular}
\end{table} 
Table 1: _Cont._Among bacterial-feeders, the most numerous genera were _Halicphalobus_ and _Acrostichus_. Depending on the type of compost and composting time, the number of _Acrostichus_ sp. ranged from 1.9% to 72.9%. In the compost made of sewage sludge and sawdust during composting, the abundance of _Acrostichus sp._ decreased from 59.2% to 46.9%. The change in sewage sludge for garden and park waste reversed this relationship and during composting, abundance of this genus increased from 1.9% to 25.6%. In compost made from sewage sludge, garden and park waste and sawdust, changes in the abundance of _Acrostichus_ sp. mirrored those in the compost prepared from sewage sludge and sawdust. The number of sequence reads decreased from 72.9% to 49.9%. After their introduction into the compost, _E. fetida_ led to a noticeable increase in abundance of _Acrostichum_ sp. In the compost, there was a detected presence of _A. floridensis._ An abundance of this species varied from <0.1% to 30.5%. To our knowledge, this was the first time the species was reported in compost. Previous research suggested that earthworms could influence the structure of nematode communities and decrease nematode density by engaging in grazing and ingesting nematodes. The trophic groups only consisted of fungivores and bacterivores since no plant-parasitic, dorylaimid, or mononchid nematodes were recovered [31; 32]. In our study, nematode communities were dominated by bacterivories and their abundance was reduced by earthworm activity in variant C5 (Figure 3E).