## 3 Results and Discussion

Table 1: The yield of hydrocolloids, chemical composition of preparations and molecular properties of extracted polysaccharides. ture [7; 8]. Based on the results obtained after the acid hydrolysis of the extracts, it can be concluded that the main polysaccharides in the preparations obtained from fenugreek seeds were galactomannans, the content of which was about 27-33% (Table 1), and this value was lower than the values presented in the literature, where the content of this polysaccharide in preparations was over 75% [8]. On the basis of the content of sugars after acid hydrolysis, polysaccharides that occurred in smaller amounts in fenugreek seed preparations can probably also include \(\beta\)-glucan composed of glucose residues and arabinoxylan composed of arabin-xylose residues (Table 1), but there is a lack of literature data to confirm these speculations. Fenugreek seed preparations PFRT and PF70 were characterized by a total sugar content of about 39% and 47%, which differs from the literature data, where the sugar content of the milled fenugreek seed preparation was about 90% [8]. The observed differences were due to the use of different raw materials, the use of a different extraction process (which included purification), as well as differences in methods of sugar analysis. Analysis of monosaccharides after the acid hydrolysis of polysaccharides extracted from milled sweet basil seeds showed that they consisted mainly of sugar residues such as galactose, glucose and mannose and in smaller amounts of xylose and arabinose (Table 1). The data presented previously in the literature only concern polysaccharides extracted from unmilled basil seeds but also indicate the presence of the mentioned sugars [13]. Based on literature data, it can be concluded that basil seed preparations could probably include such polysaccharides as galacto or/and glucomannan, arabinoxylan and arabinogalactan [34]. Polysaccharide preparations from milled sweet basil seeds were characterized by a total sugar content after acid hydrolysis of about 32% and 37% (Table 1), which was significantly different from the sugar content in preparations obtained from unmilled basil seeds by [PERSON] et al. [13], in which the sugar content was above 90%. The protein content (Table 1) of fenugreek seed preparations (PFRT and PF70) was much higher than reported in the literature [7; 9]. It should be noted that [PERSON] et al. [7] used a protease preparation during the extraction of hydrocolloids from fenugreek seeds, so that the protein content of the preparation was only 2.36%. The protein content of preparations obtained from milled sweet basil seeds (PSBRT and PSB70) was also high (Table 1). In studies by [PERSON] et al. [15] and [PERSON] et al. [13], the content of this component in hydrocolloid preparations obtained from whole, sweet basil seeds was only 2.4-10.9%, but the differences in the results were due to the different preparation of the raw material for extraction, as the studies by [PERSON] et al. [15] and [PERSON] et al. [13] used whole, non-milled basil seeds. It should be noted that the preparations obtained from the milled seeds of both fenugreek and basil by extraction with water at 70 \({}^{\circ}\)C were characterized by significantly (\(p<0.05\)) higher yields, lower protein content and higher polysaccharide content compared to analogous preparations extracted with water at room temperature. This could be explained by the partial thermal denaturation of the protein and loss of its solubility, which resulted in a decrease in its proportion in the extracts.