The basic idea that unites this extremely heterogeneous group of preparations is that by centrifuging the blood, all the elements that will promote the healing and tissue regeneration process are extracted, especially platelets (as a source of growth factors), fibrin (as a matrix), and in some cases, leukocytes [28]. Oral implantology, maxillofacial reconstruction, orthodontic therapy and regenerative periodontal procedures are all directly dependent on the success of the bone regeneration process [29]. Many studies have demonstrated the effectiveness of growth factors in promoting cell proliferation, differentiation, chemotaxis and extracellular matrix synthesis during tissue healing [30]. Autologous blood products are classified into four basic categories, based on cellular content and fibrin-fiber architecture, namely, pure platelet-rich plasma (P-PRP), leukocyte and platelet-rich plasma (L-PRP), pure platelet-rich fibrin (P-PRF) and leukocyte and platelet rich fibrin (L-PRF) [31]. To prepare these PRF products, blood is collected in test tubes without anticoagulants and immediately centrifuged. Unlike PRP, PRF does not degrade rapidly after application; on the contrary, its strong fibrin matrix is easily remodeled, like a natural blood clot. The number of platelets and leukocytes in these preparations is high, and it is important to note that they remain preserved during the procedure of preparing the product. Platelets are activated during the preparation process, which leads to a significant release of growth factors from them and from leukocytes. Its composition allows for the slow and continuous release of many growth factors (PDGF, TGF, VEGF, IGF) over a period of 7-14 days [32; 33; 34]. The fibrin L-PRF clot is often described as an \"optimized\" blood clot. L-PRF preparations are the right answer to the requirements of oral and maxillofacial surgery, because they are easily combined with the largest number of surgical techniques (as an augmentation material, as a combination with another material or as a protective membrane for healing) [35; 36; 37; 38]. Blood preparations containing concentrated growth factors are widely used today, with the aim of shortening the interval between tooth extraction and implantation [39] and bone augmentation in the maxillary sinus [40], as well as improving the success of implant therapy. The beneficial effect of these preparations in the post-extraction period is evident in terms of accelerated healing of soft tissues; reduction of pain, swelling and trismus; and stimulating formation of bone tissue [41; 42; 43]. Based on the assumption that the reduction of G-force will lead to an increase in the number of leukocytes in the PRF matrix, new protocols have been developed for the preparation of advanced platelet-rich fibrin (A-PRF and A-PRF+), injectable platelet-rich fibrin (i-PRF) and concentrated platelet-rich fibrin (C-PRF), products that have also found their clinical application [33; 44; 45]. All these preparations, because of different preparation protocols, have different characteristics. There is a lot of contradictory information in the literature about the effectiveness of different autologous platelet preparations regarding healing of soft and bone tissues after tooth extraction, which necessitates the need to conduct controlled clinical studies. There is evidence that the application of the autologous blood preparations can, to a certain extent, improve the tissue healing process and reduce the unwanted side effects of surgical intervention after the extraction of the mandibular third molar [46; 47; 48; 49; 50; 51]. Since surgical extraction of the third mandibular molar is the most frequent intervention done by oral and maxillofacial surgeons, complications and sequelae of this intervention often represent a great clinical problem. L-PRF is simple to produce and apply; it is an autologous product, so the danger of adverse reactions and transmitting diseases is eliminated.