## 3 Results and Discussion
### HPLC-PDA-tQ-ESI-MS Profiles of SPE Fractions of Silene repens: Qualitative Study
Molecular formula **13** was defined as C\({}_{32}\)H\({}_{38}\)O\({}_{19}\) ([M-H]\({}^{-}\) with \(m/z\) 725). The mass spectrum of **13** was close to schaftooside (appigenin-6-C-glucose-8-C-arabinoside, **14**), which made it possible to preliminarily characterize **13** as schaftooside O-hexoside. Previously, **13** was discovered in _S. italica_ and named schaftooside-2\({}^{\prime\prime}\)-O-glucose (sileneside E) [30]. Effect of Phytohormones and Elicitors on Productivity and Content of Edystereids in Introduced Seedlings of S. repens  
At the next stage of investigation, we decided to evaluate the effect of phytohormones and elicitors on productivity and content of ecystereids in introduced seedlings of _S. repens_. Epibrassinolide, indole-3-butyric acid, 4-chlorophenylacetic acid, gibberell acids potassium salt, arachidonic acid, and ethyl arachid in different concentrations (1, 10, 100 mg/L) were applied. The appearance of _S. repens_ seedlings (age 2 months) after 1 month of phytohormones and elicitors treatment was estimated (Figure 4). The average weight of leaves and roots per plant, as well as content of 20-hydroxycedysone (**6**) and polypodine B (**7**) as marker compounds, were also evaluated (Table 1). According to the data obtained, the treatment of _S. repens_ seedlings with epibrassino-lide at a concentration of 100 mg/L led to an increase in the average weight of plant leaves by 1.38 times. The productivity of root weight increased from 51.2 mg to 61.3 mg when using the maximum concentration of epibrassinolide. The content of 20-hydroxycedysone and polypodine B in leaves of _S. repens_ seedlings was the highest among all experimental groups and exceeded the concentrations of the same ecystereids by more than two times in comparison with the control sample. Epibrassinolide belongs to the class of brassinos  
Figure 4: The appearance of _S. repens_ seedlings (age 2 months) after 1 month of phytohormones and elicitors treatment. The letters indicate phytohormones and elicitors used (100 mg/L): **(a)**—control 1; **(b)**—control 2; **(c)**—indole-3-butyric acid; **(d)**—4-chlorophenylacetic acid; **(e)**—epibrassinolide; **(f)**—archidonic acid; **(g)**—gibberellic acids potassium salt; **(h)**—ethyl arachid. teroids, chemically being a polyhydroxysteroid that is necessary for the development of plant objects [41; 42]. The similarity in chemical structures between ecdysterioids and brassinosteroids could be the possible reason of this phenomenon [43] and could have an indirect effect on the quantitative content of each other. Thus, treatment of _Lepidium sativum_ seedlings with exogenous 20-hydroxyecdysone led to a significant decrease in the content of endogenous brassinosteroids [44]. In turn, when the seedlings were treated with epibrassinolide, the opposite effect was observed. In accordance with the chemical structure, 20-hydroxyecdysone, polypodine B, and epibrassinolide belong to tetracycline triterpenenes and include polyhydroxylated steroid structures with an oxygenated B-ring [45]. However, the following structural differences between ecdysterioids and brassinosteroids affect their biological activities: the B-ring of epibrassinolide contains only a carbonyl group at C-6, while ecdysterioids have a typical chromophore 14-hydroxy-7-en-6-one fragment; hydroxyl groups at C-2, C-3, and C-22 have a mirrored orientation; and the junction of the A- and B-rings of brassinosteroids skeleton is characterized by _trans_-configuration while ecdysterioids possess a _cis_-orientation [44; 45]. Interestingly, the data obtained correlated with literature only for leaves of _S. repens_. The content of 20-hydroxyecdysone in roots treated by epibrassinolide (100 mg/L) did not change significantly when compared with the control group (0.41 vs. 0.44 mg/g, respectively). The watering of _S. repens_ seedlings with indole-3-butyric acid at maximum concentration had no pronounced effect on yield or ecdystenoid content. The productivity of both leaves and roots of _S.