## 2 Materials and Methods  
### Molecular Genotyping of the NCYC 1146 Strain through 18s rRNA Gene Sequence  
DNA extraction from the NCYC 1146 strain was performed using Ultra Clean(r) Microbial DNA Isolation (MO BIO Laboratories INC.) (Carlsbad, CA, USA) following the supplier's instructions. DNA was stored at \(-20\)\({}^{\circ}\)C.  
The amplification of the ribosomal gene 18s rRNA was carried out by PCR, using the primers described 18S1 (5'-AACCTGTTGATCCTGCCAAT-3') and 18S12 (5'-CCITGTTACGACTTCACCTTCTCT- 3') [18]. Reaction mixture contained the following reagents: 5\(\times\) buffer of dNTPs, 2 mM of MgCl2, 1 U of Go-taq polymerase, 0.5 uM of each primers and, approximately, 1.5 uL of DNA in a final volume of 25 uL. The amplification conditions used were specified by [PERSON] et al. [18] as follows: initial denaturation at 95 \({}^{\circ}\)C for 5 min, followed by 35 amplification cycles. Each cycle consisted of denaturation at 95 \({}^{\circ}\)C for 30 s, alignment for 30 s at 55 \({}^{\circ}\)C and extension at 72 \({}^{\circ}\)C for 1 min, with a final extension step of 72 \({}^{\circ}\)C for 10 min. PCR products were visualized through 2% in agarosegel electrophoresis. The bands were visualized by gel staining with ethidium bromide (0.5 ug mL\({}^{-1}\)) and exposure with a UV transilluminator.  
Bands of expected sizes (>1500 pb) were purified using the E.Z.N.A(r) Gel Purification Kit, following the supplier's instructions. The purified PCR product was ligated to the pGEM(r)T-Easy vector (Promega) using a vector ratio of 3:1 in a final reaction volume of 10 \(\mu\)L and then incubated overnight at 4 \({}^{\circ}\)C.  
Bacterial transformation was done with a vial of competent _Escherichia coli_ cells (JM109(r) Promega) and the ligation product (recombinant plasmid), following the thermal shock methodology. The transformed bacteria were seeded in Petri dishes with LB agar and ampicillin (50 ug mL\({}^{-1}\)), IPTG (0.5 mM) and \(\beta\)-galactosidase (50 mg mL\({}^{-1}\)), which allowed for identifying the bacteria in the recombinant plasmid. The seeded plates were left to grow overnight at 37 \({}^{\circ}\)C. The positive clones were confirmed by conventional PCR in the colonies, and the bands were viewed by SafeView(tm) staining with and exposure in a UV transilluminator. The positive colonies were grown in liquid LB medium with ampicillin (50 ug mL\({}^{-1}\)), and the DNA plasmid was purified with the commercial kit commercial Plasmid Miniprep kit II E.Z.N.A.(r), following the supplier's instructions.  
Purified plasmids containing the cloned segment were sequenced by MACROGEN (South Korea). To identify the microorganisms, the sequences obtained were compared with the sequences available at the GenBank Database by the BLAST program available at the NCBI website. The phylogenetic tree based on the ribosomal gene 18s rRNA sequence was processed with the MEGA 6 and Geneious version 6.0.3 programs (Biomatters, Ltd.)  
The sequences obtained from the studied strains were entered into the GenBank database with the following access numbers: KJ530974 (C6), KJ530975 (16 CC1B), KJ530976 (NCYC 4007), KJ530977 (C3), KJ530978 (C30), KJ530979 (C46), KJ530980 (C51), KJ530981 (C24), KJ530982 (P39) and KJ530983 (C4).