## 3 Results
### Sensory Evaluation
\begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Days** & **Leaves** & **Freshness (\%)** & **Colour (\%)** & **Texture (\%)** & **Odour (\%)** & \multicolumn{2}{c}{**Overall**} \\  & & & & & & & **Appearance (\%)** \\ \hline \multirow{4}{*}{0} & Non-impregnated & 62.7 \({}^{\text{Ba}}\) & 71.7 \({}^{\text{Aa}}\) & 53 \({}^{\text{Aa}}\) & 69.3 \({}^{\text{Aa}}\) & 62.7 \({}^{\text{Aa}}\) \\  & SA-impregnated & 64.7 \({}^{\text{Aa}}\) & 70.7 \({}^{\text{ABa}}\) & 53 \({}^{\text{Ac}}\) & 64.7 \({}^{\text{Bb}}\) & 63 \({}^{\text{Ab}}\) \\  & GABA-impregnated & 63.3 \({}^{\text{Ad}}\) & 70 \({}^{\text{Bd}}\) & 51.7 \({}^{\text{Be}}\) & 64.7 \({}^{\text{Bb}}\) & 62.3 \({}^{\text{Ad}}\) \\  & Sucrose-impregnated & 61.7 \({}^{\text{Bb}}\) & 71 \({}^{\text{Ab}}\) & 51.7 \({}^{\text{Bd}}\) & 64.7 \({}^{\text{Ba}}\) & 63.3 \({}^{\text{Ac}}\) \\ \hline \multirow{4}{*}{1} & Non-impregnated & 57.7 \({}^{\text{Db}}\) & 68 \({}^{\text{Bb}}\) & 54.3 \({}^{\text{Aa}}\) & 68 \({}^{\text{Aab}}\) & 61.7 \({}^{\text{Ca}}\) \\  & SA-impregnated & 58.7 \({}^{\text{Cc}}\) & 69.7 \({}^{\text{Aab}}\) & 57.3 \({}^{\text{Bb}}\) & 65.3 \({}^{\text{Bb}}\) & 65.3 \({}^{\text{Ba}}\) \\  & GABA-impregnated & 63.7 \({}^{\text{Ad}}\) & 70 \({}^{\text{Ac}}\) & 66.3 \({}^{\text{Ab}}\) & 65.3 \({}^{\text{Bb}}\) & 70 \({}^{\text{Ab}}\) \\  & Sucrose-impregnated & 62 \({}^{\text{Bb}}\) & 70 \({}^{\text{Ac}}\) & 66.3 \({}^{\text{Ab}}\) & 65 \({}^{\text{Ba}}\) & 69.7 \({}^{\text{Aa}}\) \\ \hline \multirow{4}{*}{3} & Non-impregnated & 53.3 \({}^{\text{Cc}}\) & 65.7 \({}^{\text{Dc}}\) & 51 \({}^{\text{Db}}\) & 66 \({}^{\text{Bc}}\) & 61.7 \({}^{\text{Da}}\) \\  & SA-impregnated & 60 \({}^{\text{Cb}}\) & 69.3 \({}^{\text{Cb}}\) & 69.3 \({}^{\text{Ba}}\) & 67.3 \({}^{\text{Aa}}\) & 64.7 \({}^{\text{Ca}}\) \\  & GABA-impregnated & 64.7 \({}^{\text{Bc}}\) & 73.7 \({}^{\text{Ab}}\) & 68 \({}^{\text{Cc}}\) & 68.3 \({}^{\text{Aa}}\) & 71 \({}^{\text{Aa}}\) \\  & Sucrose-impregnated & 69.3 \({}^{\text{Aa}}\) & 72.7 \({}^{\text{Ba}}\) & 70 \({}^{\text{Aa}}\) & 65 \({}^{\text{Ba}}\) & 70 \({}^{\text{Ba}}\) \\ \hline \multirow{4}{*}{5} & Non-impregnated & 53 \({}^{\text{Dc}}\) & 66 \({}^{\text{Cc}}\) & 47 \({}^{\text{Bc}}\) & 66.7 \({}^{\text{Bc}}\) & 58 \({}^{\text{Db}}\) \\  & SA-impregnated & 59.7 \({}^{\text{Cb}}\) & 70.3 \({}^{\text{Bab}}\) & 68.7 \({}^{\text{Aa}}\) & 68.3 \({}^{\text{Aa}}\) & 64.3 \({}^{\text{Ca}}\) \\  & GABA-impregnated & 68 \({}^{\text{Aa}}\) & 75.3 \({}^{\text{Aa}}\) & 70 \({}^{\text{Aa}}\) & 68.3 \({}^{\text{Aa}}\) & 71.3 \({}^{\text{Aa}}\) \\  & Sucrose-impregnated & 62 \({}^{\text{Bb}}\) & 66.7 \({}^{\text{Cd}}\) & 69.7 \({}^{\text{Aa}}\) & 62.3 \({}^{\text{Bb}}\) & 65.7 \({}^{\text{Bb}}\) \\ \hline \multirow{4}{*}{7} & Non-impregnated & 42 \({}^{\text{Dd}}\) & 58.3 \({}^{\text{Cd}}\) & 44.3 \({}^{\text{Cd}}\) & 64.3 \({}^{\text{Bd}}\) & 51 \({}^{\text{Dc}}\) \\  & SA-impregnated & 53.3 \({}^{\text{Cd}}\) & 63 \({}^{\text{Bc}}\) & 57.7 \({}^{\text{Bb}}\) & 65.7 \({}^{\text{Bb}}\) & 56.7 \({}^{\text{Cc}}\) \\  & GABA-impregnated & 67 \({}^{\text{Ab}}\) & 71 \({}^{\text{Ac}}\) & 68.7 \({}^{\text{Ab}}\) & 68.3 \({}^{\text{Aa}}\) & 68.7 \({}^{\text{Ac}}\) \\  & Sucrose-impregnated & 57.7 \({}^{\text{Bc}}\) & 62 \({}^{\text{Be}}\) & 58.3 \({}^{\text{Bc}}\) & 62.7 \({}^{\text{Cb}}\) & 61 \({}^{\text{Bd}}\) \\ \hline \hline \end{tabular}
\end{table} 
Table 1: Effect of different impregnating solutions on freshness, color, texture, smell and overall appearance of spinach leaves during storage at 4 \({}^{\text{C}}\) for 7 days. Spinach leaves were either non-impregnated or impregnated with 2 mM SA, 5 mM GABA and 0.3 M sucrose solutions. The scores were given by 60 panelists. Capital letters represent the significant difference between impregnated compounds and small letters represent the significant difference between storage days. Figure 4: Overall visual quality of non-impregnated leaves and vacuum impregnated leaves with 2 mM SA, 5 mM GABA and 0.3 M sucrose during 7 days at 4 \({}^{\text{C}}\).