## 3 Results
### VOC Profile of C.depressa Fruit Edible Parts of Different Origins  
A total of 59 VOCs were identified from the 102 chromatographic peaks detected in the edible parts of the 23 accessions of _C.depressa_ (Supplementary Table S2). The average of the relative percentage of the identified VOCs in all accessions was 84.1% of total  
Figure 4: Volcano plots of VOCs of _C.depressa_ leaves: (**a**) comparison between Okinawa and Kagoshima accessions; (**b**) comparison between Okinawa and Taiwan accessions; (**c**) comparison between Kagoshima and Taiwan accessions. normalized intensity. The relative normalized intensities of the total volatiles were higher in Taiwanese fruits compared to Okinawa and Kagoshima, with averages of 2.03, 1.17, and 0.81, respectively (Figure 5a). Among the 23 accessions, \"Nantou\" (2.24) and \"Taoyuan\" (1.83) from Taiwan and \"Kabuchi-hybrid\" (1.70), \"Ishikunibu\" (1.63), and \"Motobu\" (1.61) from Okinawa were expected to have a strong volatile intensity, while \"Kakeroma\" (0.54) from Kagoshima was suggested to be low. Figure 5: Normalized intensities and multivariate statistical plots of VOCs in _C. depressa_ fruit edible parts: (**a**) normalized intensities of total compounds; (**b**) heat map of normalized intensities of major compounds; (**c**) PCA score plot; (**d**) PCA loading plot; (**e**) OPLS-DA score plot; (**f**) OPLS-DA loading plot. Accession numbers in (**a**,**b**) are as follows: (1) KGS-Amani Oshima, (2) KGS-Kakeroma, (3) KGS-Okinoerabu, (4) KGS-Tokunoshima 1, (5) KGS-Tokunoshima 2, (6) OKN-Hijakunibu, (7) OKN-Iriomote, (8) OKN-Ishikunibu, (9) OKN-Izumi kugani, (10)OKN-Izumi kugani-like, (11) OKN-Kaachi, (12) OKN-Kabuchi-hybrid, (13) OKN-Katsuyama kugani, (14) OKN-Kohama, (15) OKN-Motobu, (16) OKN-Nakamoto seedless, (17) OKN-Ogimi 1, (18) OKN-Ogimi 2, (19) OKN-Ogimi kugani, (20) OKN-Sokihidai, (21) OKN-Tokashiki, (22) TWN-Nantou, and (23) TWN-Taoyuan. The heat map revealed the normalized intensity of the top 10 VOCs of each of 23 accessions, and especially p-cymene, limonene, (\(Z\))-\(\beta\)-codimene, and \(\gamma\)-terpinene were highly present in \(C\). _depressa_ fruit edible parts (Figure 5b). The other 12 components, including aldehydes, esters, and monoterpenes, showed differences in content among accessions. Kagoshima accessions contained a greater amount of hexanal, while esters (ethyl butanoate, ethyl hexanoate, and ethyl acetate) were characteristic in several Okinawan fruits, such as the \"Kaachi\", \"Motobu\", \"Nakamoto seedless\", \"Ogimi 2\", and \"Sokihidai\" lines. Additionally, Taiwanese \"Nantou\" and Kagoshima \"Okinoerabu\" and \"Tokunoshima 1\" were rich in linalool; both Taiwanese accessions and the Okinawan \"Izumi kugani\" were rich in 1,8-cineole; and \"Taoyuan\" was rich in thymol methyl ether. Other monoterpene hydrocarbons were similar to the total volatile intensity results (Figure 5a); thus, the higher the amount of VOCs, the more abundant the monoterpene hydrocarbons tended to be in Taiwanese and Okinawan accessions. The PCA plot revealed the singularly divided accessions of the two Taiwanese accessions and Okinawan \"Kabuchi-hybrid\", and other accessions from Okinawa and Kagoshima origins which were not distinctively separated (Figure 5c). Specifically, Taiwanese \"Nantou\" was located in the farthest position in the quadrant of PC1-negative and PC2-negative. On the other hand, Taiwanese \"Taoyuan\" and Okinawan \"Kabuchi-hybrid\" were plotted in the quadrant of PC1-negative and PC2-positive. Moreover, there was a tendency in the PC2 axis, in which the accessions queued from positive and negative in the order of Okinawa to Kagoshima origin. Most VOCs were outlined in the negative direction of PC1 in the loading plot, indicating that Taiwanese fruits contained greater amounts of these compounds (Figure 5d).