## 5 Conclusions  
The main goal of this study was to estimate the impact of the addition of pullulan and/or disaccharides (namely sucrose and trehalose) on the encapsulation of chokeberry polyphenols in alginate hydrogel beads. Results showed that the composition of encapsulation mixture and the structure of phenolics played important roles in the entrapment of phenolics into hydrogel beads. The addition of pullulan alongside alginate in the encapsulation mixture had the most positive impact on the entrapment of anthocyanins and quercetin-3-rutinoside. The addition of pullulan and/or disaccharides negatively affected the encapsulation of neochlorogenic acids, i.e., the highest entrapment of this phenolic acid was in the alginate encapsulation mixture. The positive effect of all compounds in the encapsulation mixture was revealed in the encapsulation of chlorogenic acid. Especially high encapsulation efficiency was achieved for anthocyanins, proving that pullulan can be used in combination with alginate to encapsulate these valuable pigments. **Supplementary Materials:** The following supporting information can be downloaded at: [[https://www.mdpi.com/article/10.3390/app1516320/s1](https://www.mdpi.com/article/10.3390/app1516320/s1)]([https://www.mdpi.com/article/10.3390/app1516320/s1](https://www.mdpi.com/article/10.3390/app1516320/s1)), Figure S1. Chromatograms of alginate hydrogel beads (C-3-G: cyanidin-3-galactosidase, C-3-A: cyanidin-3-arabinoside, NcA: neochlorogenic acid, CA: chlorogenic acid, Q-3-R: quercetin-3-rutinoside); Figure S2. Chromatograms of alginate/pullulan hydrogel beads (C-3-G: cyanidin-3-galactosidase, C-3-A: cyanidin-3-arabinoside, NcA: neochlorogenic acid, CA: chlorogenic acid, Q-3-R: quercetin-3-rutinoside); Figure S3. Chromatograms of alginate/sucrose hydrogel beads (C-3-G: cyanidin-3-galactosidase, C-3-A: cyanidin-3-arabinoside, NcA: neochlorogenic acid, CA: chlorogenic acid, Q-3-R: quercetin-3-rutinoside); Figure S4. Chromatograms of alginate/trehalose hydrogel beads (C-3-G: cyanidin-3-galactosidase, C-3-A: cyanidin-3-arabinoside, NcA: neochlorogenic acid, CA: chlorogenic acid, Q-3-R: quercetin-3-rutinoside); Figure S5. Chromatograms of alginate/pullulan/sucrose hydrogel beads (C-3-G: cyanidin-3-R:cyanidin-3-galactosidaseoside, C-3-A: cyanidin-3-arabinoside, NcA: neochlorogenic acid, CA: chlorogenic acid, Q-3-R: quercetin-3-rutinoside); Figure S6. Chromatograms of alginate/pullulan/trehalose hydrogel beads (C-3-G: cyanidin-3-galactosidaseoside, C-3-A: cyanidin-3-arabinoside, NcA: neochlorogenic acid, CA: chlorogenic acid, Q-3-R: quercetin-3-rutinoside); Figure S7. Chromatograms of cyanidin-3-glucoside solutions; Figure S8. Chromatograms of cyanidin-3-arabinoside solutions; Figure S9. Chromatograms of chlorogenic acid solutions; Figure S10. Chromatograms of neochlorogenic acid solutions; Figure S11. Chromatograms of quercetin-3-rutinoside solutions; Figure S12. Representative photo of formulated hydrogel beads. Conceptualization, M.K. and J.S. ; methodology, M.K. and A.P. ; formal analysis, I.C. and A.P. ; investigation, I.C., M.K. and A.P. ; data curation, I.C. and A.P. ; writing--original draft preparation, M.K. ; writing--review and editing, J.S. ; supervision, M.K. and A.P. ; project administration, M.K. ; funding acquisition, M.K. All authors have read and agreed to the published version of the manuscript. This work was supported by the Croatian Science Foundation under the project (IP-2019 04-5749) \"Design, fabrication and testing of biopolymer gels as delivery systems for bioactive and volatile compounds in innovative functional foods (bioACTIVEgels)\", and Young Researchers' Career Development Project--Training New Doctoral Students (DOK-2020-01-4205). Not applicable. The original contributions presented in this study are included in the article and Supplementary Materials. Further inquiries can be directed at the corresponding author.