## 3 Results  
Firstly, the different peripheral blood eosinophil counts cut-off points were examined for patients with asthma. Statistically significant differences were found in every selected cut-off point (150, 300, 500 and 800) for some items (Table 1). Nevertheless, there were important differences between the sensibility of these thresholds when detecting them. Furthermore, 300 Eos/uL was the cut-off point that detected more statistically significant differences and indicated a higher significance, followed by 500, which also objectified these differences with a high sensibility (Tables 1-3). It was found that 150 Eos/uL is less effective in detecting these changes and 800 Eos/uL does not detect most differences and its statistical significance is markedly lower. The results were analyzed by comparing sexes and age strata (19-39, 40-64 and >65 years), with no notable differences. When looking into patients without asthma, we observed that a 150 Eos/uL cut-off point was able to detect differences in drug (probability value (\(p\)) 0.033, relative risk (RR) 0.408, odds ratio (OR) 0.389) and contact allergies (\(p\) 0.017, RR 4.438, OR 4.567). Moreover, rhinitis (\(p\) 0.017, RR 0.894, OR 0.606) and atopy (\(p\) 0.023, RR 0.408, OR 0.389) are more common in patients with more than 150 eosinophils, showing a higher risk of developing these conditions. It is also remarkable that women are always more prevalent in all the eosinophil strata. However, men increase their prevalence when eosinophils are higher, showing statistically significant differences between genders when using 150 (\(p\) 0.005, RR 0.673, OR 1.750) and 300 (\(p\) 0.009, RR 0.530, OR 1.767). FeNO is also consistently higher in higher eosinophil groups (150 \(p\) 0.015, 300 \(p\) 0.001, 500 \(p\) 0.001 and 800 \(p\) 0.005), whilst Total IgE comorbidities and functional pulmonary tests remained stable despite the eosinophil counts. Looking at all studied populations, including patients with and without asthma, women are also more frequent in every studied cut-off point. However, men become more prevalent when peripheral blood eosinophil counts increase (Table 2). Asthma diagnosis also increases in higher eosinophil groups for every cut-off point aside from 800, showing a decrease in the risk of developing this condition when eosinophil counts are lower (150 \(p\) 0.001, OR 0.540, RR 0.749; 300 \(p\) 0.001, OR 0.513, RR 0.768; 500 \(p\) 0.001, OR 0.391, RR 0.721). The tendency can be observed in the 800 Eos/uL, but risk and statistical differences were not significant. Rhinitis (150 \(p\) 0.001, RR 0.914, OR 0.508; 300 \(p\) 0.003, RR 0.935, OR 0.513; 500 RR 0.949) and atopy (150 \(p\) 0.001, RR 0.863, OR 0.499; 300 \(p\) 0.001, RR 0.886, OR 0.472; 500 \(p\) 0.002, RR 0.885, OR 0.426) are also more frequent when eosinophils are elevated, having a lower risk of existing when eosinophils decrease. When examined for asthma patients, and contrary to non-asthmatics, total IgE and FeNO were found to be elevated in the groups with higher eosinophil count (\(p\) = 0.001) in all cut-off points for FeNO and p between 0.001 and 0.003 for IgE, except at the 800 Eos/uL threshold where total IgE was higher in the >800 Eos/uL but not significantly. Moreover, in this sample we can observe changes in spirometric values. For the 150 Eos/uL threshold, we observed differences in FEV1% (\(p\) 0.001), FEV0% (\(p\) 0.033), FEV1/FVC (\(p\) 0.010), FEV1/FVC Zscore (\(p\) 0.002) and FEF 25-75% (\(p\) 0.007). Additionally, the bronchodilation test results led to more positive results in the group >150 Eos/uL (\(p\) 0.030). This tendency can also be observed in other cut off points such as 300 Eos/uL FEV1% (\(p\) 0.001), FVC% (\(p\) 0.006), FEV1/FVC (\(p\) 0.001), FEV1/FVC Zscore (\(p\) 0.001), FEF 25-75% (0.001) and bronchodilation test (0.019) and with 300 Eos/uL FEV1% (\(p\) 0.004), FVC (0.047), FEV1/FVC (\(p\) 0.002), FEV1/FVC Zscore (\(p\) 0.001), FEF 25-75% (\(p\) 0.001) and the bronchodilation test result (0.047).