## 3 Results  
### Applied to QTL Mapping of Phenotype and Secondary Metabolites  
In order to distinguish the resistance and susceptibility of the CNDH lines to WBPH, 14 days after seeding, the WBPH was treated and the phenotypes were compared. When treated with WBPH, resistant plants showed green leaves, but susceptible lines revealed dried leaves. The resistance score was assigned to the CNDH lines through the comparison of phenotypes after treatment with WBPH. In addition, HPLC analysis was performed to compare the secondary metabolites produced in the resistant and susceptible lines after inoculation with WBPH. The CNDH lines showed resistance and susceptible when inoculated with WBPH, and as a result of HPLC analysis, peaks appeared different at specific times and showed various concentrations (Supplementary Materials Figure S1). The concentration of the substance displayed when treating the CNDH lines of WBPH shows a continuous distribution showing a normal distribution, and it means a quantitative trait expression regulated by one or more genes. QTL mapping was performed using thedata collected from 2016 to 2020. First, check the marker polymorphic in Cheongcheong and Nagdong were performed using 423 SSR markers for high-density mapping of the CNDH lines. Of these, 218 (52%) SSR markers are polymorphic. Of the 218 SSR markers, 198 SSR markers were used except that the positions on the chromosome were overlap or unknown, and a related map was created. The total length of the related maps is 2121 cM and the average distance between SSR markers is 10.6 cM. It is the result of QTL related to resistance to WBPH for 5 years in the CNDH lines (Figure 1A and Supplementary Materials Table S1). In 2016, qwbph2, qwbph4, qwbph6, qwbph7 and qwbph8 were detected. The qwbph2 is detected in RM5619-RM424 on chromosome 2, with a LOD value of 2.5 and an explainable phenotypic change of 10%. The qwbph4 is detected by RM273-RM16467 on chromosome 4 with a LOD value of 4.7 and an explainable phenotypic change of 18%. The qwbph6 is detected by RM50-RM1163 on chromosome 6 with a LOD value of 6.1 and an accountable phenotypic change of 29%. The qwbph7 is detected by RM21972-RM6776 on chromosome 7, with a LOD value of 4.8 and an explainable phenotypic change of 18%. The qwbph8 was detected by RM3689-RM23314 on chromosome 8 with a LOD value of 5.2 and an explainable phenotypic change of 18%. In 2017, qwbph4-1, qwbph6-1 and qwbph6-2 were detected. The qwbph4-1 is detected by RM127-RM17502 on chromosome 4 with a LOD value of 2.6 and an explainable phenotypic change of 16%. The qwbph6-1 is detected by RM50-RM1163 on chromosome 6 with a LOD value of 4.3 and an explainable phenotypic change of 23%. The qwbph6-2 is detected by RM20196-RM20096 on chromosome 6 with a LOD value of 2.7 and an explainable phenotypic change of 11%. In 2018, qwbph4-2, qwbph7-1, qwbph8-1, qwbph8-2 and qwbph12 were detected. The qwbph4-2 is detected by RM280-RM6909 on chromosome 4 with a LOD value of 3.5 and an explainable phenotypic change of 30%. The qwbph7-1 is detected by RM248-RM1134 on chromosome 7 with a LOD value of 3.0 and an explainable phenotypic change of 30%. The qwbph8-1 is detected by RM23230-RM3689 on chromosome 8 with a LOD value of 2.5 and an explainable phenotypic change of 30%. The qwbph8-2 is detected by RM17699-RM264 on chromosome 8 with a LOD value of 3.3 and an explainable phenotypic change of 30%. The qwbph12 was detected to RM1226-RM12 on chromosome 12, with LOD value of 2.7 and 40% phenotypic change explained. In 2019, qwbph1, qwbph1-1, qwbph1-2, qwbph8-3 were detected. The qwbph1 is detected by RM3482-RM11966 on chromosome 1 with a LOD value of 4.0 and an explainable phenotypic change of 30%. The qwbph1-1 is detected by RM3709-RM11694 on chromosome 1 with a LOD of 3.5 and a 30% explainable phenotypic change. The qwbph1-2 is detected by RM11694-M11669 on chromosome 1 with a LOD value of 3.3 and an explainable phenotypic change of 30%.