## 3 Results
### Identification of Selected Psychrotolerant Bacteria  
The 70 selected isolates were identified through the analysis of the partial 16S rRNA gene sequence (Figure 2). The isolates belong to four bacterial phyla. The bacterial phylum with the highest diversity of genera was Actinobacteria with 19 identified strains from seven genera (_Pseudartlrobacter_, _Arttbroacter_, _Brachybacterium_, _Cutbobacterium_, _Paenarthrobacter_, _Frontalhibtains_, and _Clavibacter_), followed by Proteobacteria phylum with 43 identified strains from six genera (_Pseudomonas_, _Stenotrophomonas_, _Serratia_, _Janthinobacterium_, _Xanthomonas_, and _Brevundimonas_), Firmicutes phylum with six identified strains from three genera (_Sporosarcina_, _Solibacillus_, and _Bacillus_), and Bacteroidetes phylum, with two identified strains from two genera (_Flavobacterium_ and _Pedobacter_). The most abundant isolates corresponded to _Pseudomonas_ (18), _Stenotrophomonas_ (17), _Pseudartlrobacter_ (6), _Serratia_ (5), _Arthrobacter_ (4), and _Sporosarcina_ (4) genera. Strains from _Pseudomonas_, _Stenotrophomonas_, _Sporosarcina_, _Cutbobacterium_, _Arthrobacter_, and _Pseudartlrobacter_ genera were isolated from rhizosphere and phyllosphere. On the other hand, _Serratia_, _Xanthomonas_, _Peaarthbroacter_, and _Janthinobacterium_ genera were only isolated from rhizosphere, while _Solibacillus_, _Bacillus_, _Brachybacterium_, _Frontalabitans_, and _Clavibacter_ genera were only isolated from the phyllosphere.  
\begin{table}
\begin{tabular}{c c c c c c c c c c c c c c c c} \hline \hline  & \multicolumn{10}{c}{**Number of Isolates**} \\ \cline{2-13}  & \multicolumn{10}{c}{**Rhizosphere**} \\ \cline{2-13}
**Wild plant (Code for Isolates)** & **Total** & **Id** & **L** & **M** & **H** & **VH** & **E** & **Total** & **Id** & **L** & **M** & **H** & **VH** & **E** \\ \hline _Calycen_ sp. (Cp) & 16 & 11 & 0 & 5 & 0 & 4 & 7 & 3 & 3 & 0 & 0 & 1 & 0 & 2 \\ _Thlispi_ sp. (Tm) & 17 & 13 & 3 & 1 & 4 & 7 & 2 & 10 & 8 & 0 & 2 & 2 & 2 & 4 \\ _Haplopapus_ sp. (Hs) & 9 & 3 & 0 & 6 & 1 & 0 & 2 & 2 & 1 & 1 & 0 & 0 & 1 & 0 \\ \hline _Baccharis_ sp. (Bl) & 10 & 3 & 1 & 6 & 0 & 3 & 0 & 0 & 0 & 0 & 0 & 0 & 0 & 0 & 0 \\ _Guaphlium_ sp. (Gc) & 18 & 9 & 3 & 6 & 4 & 2 & 3 & 2 & 2 & 0 & 1 & 0 & 1 \\ \hline _Gaultheria_ sp. (Gp) & 8 & 1 & 1 & 6 & 0 & 1 & 0 & 2 & 1 & 1 & 0 & 0 & 1 \\ _Nothofagus_ sp. + _Lusnea_ sp. (NU) & 7 & 2 & 0 & 5 & 1 & 1 & 0 & 1 & 0 & 1 & 0 & 0 & 0 & 0 \\ Orites sp. (Om) & 3 & 1 & 1 & 0 & 1 & 0 & 0 & 0 & 0 & 0 & 0 & 0 & 0 & 0 \\ _Chusqua_ sp. (Cc) & 10 & 3 & 1 & 6 & 2 & 0 & 1 & 1 & 0 & 0 & 1 & 0 & 0 & 0 \\ \hline _Berberis_ sp. (Bm) & 5 & 3 & 0 & 2 & 1 & 0 & 2 & 6 & 6 & 0 & 0 & 1 & 2 & 3 \\ _Nothofagus_ sp. (Nd) & 0 & 0 & 0 & 0 & 0 & 0 & 0 & 0 & 0 & 0 & 0 & 0 & 0 & 0 \\ \hline Total isolates & **103** & **49** & **10** & **44** & **13** & **19** & **17** & **27** & **21** & **3** & **3** & **5** & **5** & **11** \\ \hline Id, Identified isolates showing \(\geq\)33% bacterial cell survival (BCS); L, low (<10\% BCS); M, moderate (10–32% BCS); H, high (33–55% BCS); VH, very high (56–78% BCS) E, excellent (79–100% BCS). & & & & & & & & & & & & \\ \hline \end{tabular} _Brevundomonas_ (R) _Janthinobacterium_ (R) _Serratia_ (R) _Stenotrophomonas_ (P) _Stenotrophomonas_ (R) _Stenodonomas_ (P) _Stedomonas_ (R) _Stenodonomas_ (R) _Stenodonomas_ (P) _Stenodonomas_ (R) _Stenobacter_ (P) _Frontalabitans_ (P) _Curtobacterium_ (P) _Stenobacterium_ (R) _Stenobacterian_ (P) _Stenobacterian_ (P) _Stenotrans_ (R) _Stenobacterian_ (P) _Stenotrans_ (R) _Stenotrans_ (P) _Stenotrans  
Of the 70 isolates, only two were closely related to phytopathogenic bacteria. The strain HsR18 that was isolated from the rhizosphere of _Haplopappus_ sp. is closely related to _Xanthomonas translucens_[60; 61]. The strain BmP21 that was isolated from the phyllosphere of _Berberis_ sp. is closely related to _Clavibacter michiganensis_[62; 63; 44]. Both strains HsR18 and BmP21 were discarded for further analysis.