## 2 Materials and Methods
### Severity  
In total, 29 (17.1%) injuries were severe, while 140 (82.9%) were non-severe.  
In the _ACTN3_ c.1729C>T polymorphism, the genotype distribution differed between severely injured and non-severely injured players (\(p\) < 0.001), showing in the TT genotype a higher frequency in players with severe injuries than those with non-severe injuries (51.8%  
\begin{table}
\begin{tabular}{l l l l l l l l l l l l} \hline \hline  & \multicolumn{4}{c}{**Injury Setting**} & \multicolumn{4}{c}{**Severity**} \\ \cline{3-11}  & \multicolumn{2}{c}{**Polymorphism Genotype**} & \multicolumn{2}{c}{**Training, n (\%)**} & \multicolumn{2}{c}{**Match, n (\%)**} & \multicolumn{2}{c}{\(p\) **Value**} & \multicolumn{2}{c}{**df**} & \multicolumn{2}{c}{**No, n (\%)**} & \multicolumn{2}{c}{**Yes, n (\%)**} & \multicolumn{2}{c}{\(p\) **Value**} & \multicolumn{2}{c}{**df**} \\ \hline \multirow{3}{*}{_ACE_} & \multirow{3}{*}{I/D} & DD & 23 (32.8) & 47 (47.5) & & & 55 (39.3) & 14 (48.3) & & & \\  & ID & 32 (45.7) & 37 (37.4) & 0.427 & 14 & 61 (43.6) & 9 (31.0) & 0.683 & 5 \\  & II & 15 (21.5) & 15 (15.1) & & & & 24 (17.1) & 6 (20.7) & & & \\ \hline \multirow{3}{*}{_ACTN3_} & \multirow{3}{*}{c.1729C>T} & CC & 23 (32.8) & 29 (29.3) & & & 50 (35.7) & 3 (10.3) & & & \\  & CT & 38 (54.3) & 58 (58.6) & 0.937 & 8 & 85 (60.7) & 11 (37.9) & \textless{}0.001 & 4 \\  & & TT & 9 (12.9) & 12 (12.1) & & & 5 (3.6) \({}^{\downarrow}\) & 15 (51.8) \({}^{\uparrow}\) & & & \\ \hline \multirow{3}{*}{_AMPD1_} & \multirow{3}{*}{c.34C>T} & CC & 38 (54.3) & 46 (46.5) & & & 61 (43.6) \({}^{\uparrow}\) & 1 (3.4) \({}^{\downarrow}\) & & \\  & CT & 18 (25.7) & 38 (38.4) & 0.465 & 13 & 50 (35.7) & 6 (20.7) \({}^{\uparrow}\) & \textless{}0.001 & 2 \\  & & TT & 14 (20.0) & 15 (15.1) & & & 29 (20.7) \({}^{\downarrow}\) & 22 (75.9) \({}^{\uparrow}\) & & & \\ \hline \multirow{3}{*}{_CKM_} & \multirow{3}{*}{c.***800A>G} & GG & 20 (29.2) \({}^{\uparrow}\) & 6 (6.1) \({}^{\downarrow}\) & & & 20 (14.6) & 5 (17.3) & & & \\  & GA & 23 (33.3) & 32 (32.3) & 38 (27.1) & 13 (44.8) & 0.199 & 4 \\  & AA & 27 (37.5) & 61 (61.6) & & & 82 (58.3) & 11 (37.9) & & & \\ \hline \multirow{3}{*}{MLCK} & \multirow{3}{*}{c.49C>T} & CC & 52 (74.3) & 75 (75.8) & & & 114 (81.4) & 17 (58.6) & & \\  & CT & 15 (21.4) & 20 (20.2) & 0.843 & 2 & 23 (16.4) & 12 (41.4) & 0.238 & 2 \\ \cline{1-1}  & & TT & 3 (4.3) & 4 (4.0) & & 3 (2.1) & 0 (0.0) & & & \\ \cline{1-1} \cline{2-11}  & \multirow{3}{*}{c.37885C>A} & AA & 1 (1.4) & 2 (2.0) & & & 2 (1.4) & 2 (6.7) & & \\ \cline{1-1}  & & CA & 5 (7.1) \({}^{\downarrow}\) & 30 (30.3) \({}^{\uparrow}\) & 0.024 & 2 & 33 (23.6) & 9 (31.0) & 0.301 & 2 \\ \cline{1-1}  & & CC & 64 (91.5) & 67 (67.7) & & & 105 (75.0) & 18 (62.3) & & \\ \hline \hline \end{tabular}  
\({}^{\uparrow}\) indicates that the frequency was above the expected value, calculated from the standardised residuals of a chi-square test; \({}^{\downarrow}\) indicates that the frequency was below the expected value, calculated from the standardised residuals of a chi-square test; df, degrees of freedom.  
\end{table}
Table 5: Genotype distribution in women’s amateur football players regarding injury setting and severity.  
vs. 3.6%) (Table 5). Regarding the _AMPD1_ c.34C>T polymorphism, differences in genotype distribution were observed in the TT genotype, with a higher frequency in players with severe injuries than those with non-severe injuries (75.9% vs. 20.7%) (_p_ < 0.001) (Table 5).