## 2 Results and Discussion
### Analysis of the Volatile Fraction
#### 2.1.3 GC-MS Analysis of EOs  
A comprehensive GC-MS analysis was carried out using two columns of different polarities, namely, HP-5 MS and DB-WAX, to obtain an overview of the chemical profiles of the nine EOs. The results of the two analyses were comparable, confirming the presence of the same main compounds in each variety. Interestingly, the more apolar HP-5 MS stationary phase allowed us to obtain, for several components, higher relative abundances, with respect to those found by employing the more polar DB-WAX column. For this reason, Table 2 reports the percentage values provided by the analysis performed with the HP-5 MS column. \begin{table}
\begin{tabular}{c c c c c c c c c c} \hline \multicolumn{2}{c}{**Compound**} & \multicolumn{6}{c}{**Hemp Variety**} \\ \hline  & **White** & \begin{tabular}{c} **Lemon** \\ **Canti** \\ **Kush New** \\ \end{tabular} & \begin{tabular}{c} **Lemon** \\ **Canti** \\ **Kush** \\ \end{tabular} & \begin{tabular}{c} **Venom** \\ **OG** \\ \end{tabular} & **Pablito** & **24 K** & \
\begin{tabular}{c} **Fresh** \\ **Mountain** \\ \end{tabular} & **Cowies** &
\begin{tabular}{c} **Gorilla** \\ **Glue** \\ \end{tabular} \\ \hline  & & & & **(g/100 g)** & & & & & & \\ \hline \(\alpha\)-pinene & 8.53 & 3.24 & 11.48 & 21.19 & 8.35 & 0.73 & 9.32 & 21.16 & 0.74 \\ \(\beta\)-pinene & 3.59 & 2.78 & 4.67 & 4.79 & 3.21 & 0.68 & 3.66 & 7.45 & 0.82 \\ myrcene & 20.28 & 11.14 & 17.13 & 10.57 & 12.56 & 8.12 & 27.46 & 29.23 & 7.16 \\ limonene & 9.17 & 5.97 & 3.38 & 4.28 & 3.30 & 4.18 & 4.10 & 2.20 & 6.79 \\ \(1.8\)-cineole & 0.07 & 0.13 & 0.04 & 0.07 & 0.03 & 0.02 & 0.04 & 0.04 & 0.02 \\ (\(E\))-\(\beta\)-ocimene & 0.10 & 4.51 & 0.16 & 0.05 & 0.06 & 0.03 & 0.02 & 0.02 & 0.03 \\ terpinolene & 9.51 & 30.47 & 1.33 & 1.65 & 0.52 & 3.69 & 0.25 & 0.18 & 0.27 \\ (\(E\))-caryophyllene & 10.79 & 10.40 & 11.91 & 17.19 & 18.15 & 18.12 & 19.70 & 18.91 & 18.94 \\ humulene & 2.96 & 3.04 & 4.46 & 4.64 & 6.82 & 5.35 & 5.51 & 8.57 & 4.86 \\ caryophyllene oxide & 3.52 & 3.34 & 3.88 & 5.60 & 7.97 & 8.00 & 1.28 & 3.93 & 6.62 \\ CBD & 5.54 & 2.91 & 4.26 & 5.25 & 3.13 & 4.02 & 3.86 & 2.30 & 3.06 \\ \(\Delta\)-9-THC & 0.13 & 0.09 & 0.10 & 0.13 & 0.12 & 0.12 & 0.12 & 0.20 & 0.20 \\ \hline Yields (\%) & 0.64 & 1.51 & 1.03 & 0.86 & 0.49 & 1.08 & 1.23 & 1.81 & 1.33 \\ \hline \end{tabular}
\end{table} 
Table 1: GC-FID analysis results for the 9 commercial varieties’ EOs and their yield values (%). \begin{table}
\begin{tabular}{l l c c c c c c c c c c c c c c c} \hline \hline  & & & & & & & & & & & & & & & & & & \\  & & & & & & & & & & & & & & & & & & \\ \hline \multicolumn{12}{c}{**Variety**} & \multicolumn{1}{c}{**Impact of**} \\  & & & & & & & & & & & & & & & & & & & & \\  & & & & & & & & & & & & & & & & & & \\  & & & & & & & & & & & & & & & & & & \\ \hline \multicolumn{12}{c}{**Variety**} & \multicolumn{1}{c}{**Impact of**} \\  & & & & & & & & & & & &  
\begin{table}
\begin{tabular}{c c c c c c c c c c c c c c c} \hline \hline \multirow{2}{*}{**N**} & \multirow{2}{*}{**Component**\({}^{*}\)} & \multirow{2}{*}{**RI**\({}  
Among the monoterpenes, \(\alpha\)-pinene, myrcene, and terpinolene were again the most abundant compounds detected in the EOs from the nine commercial varieties. Only one of them, namely Venom OG, showed prevalence of \(\alpha\)-pinene over the other terpen, as reported in the GC-FID analysis (Section 2.1.2). Indeed, this component accounted for 23.3% of the whole chemical profile, followed by (\(E\))-aryophyllene (17.1%). Myrcene was the predominant constitment of the EOs from Amnesia Cookies, Fresh Mountain, White Shark, and Lemon Conti Kush (27.1%, 26.0%, 21.5%, and 14.9% of the total composition, respectively) confirming the GC-FID outcomes (Table 1). Along with myrcene, Amnesia Cookies' EO was characterized by a significant content of \(\alpha\)-pinene (24.6%) and (\(E\))-aryophyllene (15.6%).