## 3 Results and Discussion
### Assessment of Bacterial Contamination of Air-Qualitative Analysis Using a Metagenomic Approach
\begin{table}
\begin{tabular}{c c c c} \hline \hline \multirow{2}{*}{**Row No.**} & \multirow{2}{*}{**Media Combination***} & **Percent of** & \\  & & **All Genera** & **Genera Detected** \\  & & **Detected** \\ \hline \multirow{7}{*}{1} & \multirow{7}{*}{FA} & \multirow{7}{*}{64.62\%} & _Acinetobacter, Aerococcus, Agroccocus, Algoriophagus, Amaricoccus, Arlrhrobacter, Aureimonas, Bacillus, Bosea, Brachybacterium, Brevibucillus, Brevibucerium, Brevundimonas, Candidatus_ & \\  & & & _Paracaedibacter, Carnobacterium, Caulobacter, Chryseobacterium, Corynebacterium, Cupriavidus, Devasia, Dyudobacter, Enhydrobacter, Entorecoccus, Exigobacterium, Ficthicillus, Flavulinbacter, Flaobacterium, Glutamicbacter, Cordonia, Hydrogromophaga, Hymenoptera, Jeqtgalococcus, Kocura, Kytococcus, Lactococcus, Luteimonas, Lysinibacillus, Lysobacter, Macrooccus, Marmoricola, Massilia, Methylobacterium, Microbacterium, Micrococcus, Mycobacterium, Nakamuraella, Nocandioides, Novoshingobium, Oceanobacillus, Paenanthrobacter, Penaibillus, Paeniglutantitchacter, Penaiprosaccrina, Pantoet, Paracoccus, Peduobacter, Pseudomonas, Pseudorhodobacter, Pseudoxanthomonas, Psychobacillus, Psychobacter, Ralstonia, Rhizobacter, Rhizobium, Rhodobacter, Rhodococcus, Rhodoferax, Rosemonas, Rothia, Rummelihucillus, Skermanella, Sphingobacterium, Sphingobium, Splicingomonas, Sphingoryaxis, Sphingorhabus, Sporosarcina, Staphylococcus, Stenotrophomonas, Streptococcus, Trichococcus, Truepera, Variovorax, Williams_ \\ \hline \multirow{7}{*}{2} & \multirow{7}{*}{FA, R2A} & \multirow{7}{*}{80.77\%} & Row no. 1 + _Aeromicobium_, Agromoves, Cellulomonas, Claobacterium, Cluonella, Curtobacterium, Deinococcus, Dermococcus, Dietzia, Escherichia-Shigella, Microvigra, Orntihillucillus, Pansagefibtchater, Planomicribolium, Porphyrobacter, Pseudardrhbacter, Pseudodexibacter, Sanguiuchater, Shinella, Solibacillus, Timonella_ \\ \hline \multirow{7}{*}{3} & \multirow{7}{*}{FA, R2A, LB} & \multirow{7}{*}{89.23\%} & Row no. 2 + _Achromobacter_, Clostridioides, Desemza, Dominikallus, Enterobacter, Gracillobacillus, Kaistia, Leucobacter, Terribacillus, Vagococcus, Verticia_ \\ \hline \multirow{7}{*}{4} & \multirow{7}{*}{FA, R2A, ULB, NA} & \multirow{7}{*}{93.85\%} & Row no. 3 + _Aneurinibacillus_, Bergeyella, Cellulosinicobium, Nosocomialoccus, Phenylobacterium, Streptomyces \\ \hline \multirow{7}{*}{5} & \multirow{7}{*}{FA, R2A, LB, NA, BA} & \multirow{7}{*}{96.92\%} & Row no. 4 + _Acidoorax_, _Lechevalieria_, _Ochrobactrum_, _Pronicromospora_ \\  & & & & \\ \hline \multirow{7}{*}{6} & \multirow{7}{*}{FA, R2A, LB, NA, BA, BHI} & \multirow{7}{*}{99.23\%} & Row no. 5 + _Dermabacter_, _Marinilatichacillus_, _Virgibacillus_ \\  & & & & \\ \hline \multirow{7}{*}{7} & \multirow{7}{*}{FA, R2A, LB, NA, BA, BHI, YEA} & \multirow{7}{*}{100\%} & Row no. 6 + _Leuconostoc_ \\ \cline{1-1}  & & & & \\ \hline \end{tabular}  
* NA—nutrient agar, LB—lysogeny broth, BHI—brain heart infusion agar, BA—Blickfeldt agar, FA—Frazier agar, R2A—Resoncer’s Za agar, YEA—yeast extract agar. ** The percentage of all genera detected is equal to the number of genera detected on a given medium or media combination, divided by the summarized number of unique genera grown on at least one tested medium.  
\end{table} 
Table 2: Summary of the bacterial genera recovery using various media combinations. to use more than three different media during regular air contamination assessments in museums. Therefore, it is critical to establish reliable and comparable protocols (including medium selection) for classical analyses when employing microbial cultivation. In order to obtain the most comprehensive results, based on our findings, we suggest starting with the FA medium and then, depending on the available resources, adding other media in the following order: R2A, LB, NA, BA, BHI, and YEA.