## 4 Conclusions  
The ATR-FTIR spectrum of potato flesh, although dominated by the high content of moisture and starch in the tubers, which may hide the potential role of minor constituents, provides useful information on the origin of potato accessions. Chemometric treatment of the ATR-FTIR spectra allowed for discrimination of the potato cultivars with a good accuracy. These results confirmed the great potentiality of mid-infrared spectroscopy toward tracing foodstuffs. Because of the low cost, easy use, and minimal sample manipulation, ATR-FTIR can be preferred to more sophisticated instrumental techniques used for the varietal/geographical discrimination of cultivars.  
The results obtained in this study are useful for the characterization and valorization of local germplasm. In particular, the molecular markers suggest that the potato accession named Montenerodomo, cultivated in Majella National Park, can be considered a local variety and can be registered into the Regional Voluntary GR Register and entered into the foreseen protection scheme, as reported by the Italian regional laws [48].  
Figure 7: Importance of variables in PLS-DA: variables with VIP \(>\) 1 (red) and VIP \(<\) 1 (blue).  
**Author Contributions:** Conceptualization, A.A.D. and L.D.M.; methodology and validation, R.A., E.A., F.V., and A.A.D.; software, R.G., R.T., and F.D.D.; formal analysis and data curation, R.G., R.T., and F.D.D.; investigation, R.G., R.T., V.D.C., and F.D.D.; resources, M.D.S., G.M., V.D.C., and L.D.M.; data curation, R.G.; writing--original draft preparation, review and editing, A.A.D.; project administration, M.D.S. and G.M.; funding acquisition, L.D.M. All authors have read and agreed to the published version of the manuscript.  
**Funding:** This research was funded by Majella National Park, within the Project \"Tipizzazione di specie vegetali endemic, crop wild relatives e varieta agricole autoctone del Parco della Majella mediante metodi analitici ed approcci statistici multivariati\" with the Dipartimento di Scienze Fisiche e Chimiche, Universita degli Studi dell'Aquila, and within the Project \"Coltiviamo la diversita-Caratterizzazione e conservazione del germoplasma agricolo autoctono del Parco Nazionale della Majella\" with the Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Universita degli Studi di Perugia.  
**Conflicts of Interest:** The authors declare no conflict of interest.  
\begin{table}
\begin{tabular}{c c c c c c} \hline
**Locus** & **Motif** & **Primer** & **Ta** & **Map** & **Total Found Alleles** \\ \hline \multirow{3}{*}{STMS121} & \multirow{3}{*}{(TGT)\({}_{5}\)} & FW: & & & \\  & & CACCGGAATAAGCCGATCT & 48 & XII & 301, 305, 308 \\  & & RW: & & & \\  & & TCTCCTTCCATTTTGCTG & & & \\  & & FW: & & & \\ STI0001 & (AAT)\({}_{n}\) & CACCAAAATCAGAA CCCGAT & 60 & IV & 196, 199, 202, 205, 207, 210 \\  & & RGATCATCAAATTCCCCCT & & & \\  & & FW: & & & \\ STM1064 & (TA)\({}_{12}\)-(TG)\({}_{4}\) GT (TG) & GTTTTTGGCTGTTTC & 55 & II & 207, 210, 212 \\  & & RW: & & & \\  & & TTATTTCTCTTGTGTGTGT & & & \\  & & FW: & & & \\ STG0016 & (AGA)\({}_{8}\) & ACCTGCTCACCATCAGAGA & 55 & I & 142, 149, 152, 155, 173 \\  & & RW: & & & \\  & & ACACCACCACCACCACCATCATC & & & \\  & & FW: & & & \\ STPoAC58 & (TA)\({}_{13}\) & TTGATGAAAGGAAATCCGCTTG & 55 & V & 250, 264 \\  & & RW: & & & \\  & & ACGTTAAAAGAGTAGAGGTACGC & & & \\ \hline \end{tabular}
\end{table}
Table 11: List of microsatellite loci used for genotyping the eight potato accessions. For each locus, the repeated pattern, the primer sequences, the annealing temperature (Ta), the chromosome position, and alleles found are reported.