## 3 Results
### Physicochemical Properties
#### 3.1.4 EO Content in CE EO and NLC EO  
The EO content incorporated in CE EO was 8.57 \(\pm\) 0.15 mg/mL, while in NLC EO, it was 7.75 \(\pm\) 0.05 mg/mL (\(p\) < 0.05). The ingredients with the highest content in both CE EO and NLC EO were eucalypt, linalool, camphor, and linalyl acetate. Cymene, borneol,  
Figure 1: Stability study monitoring the droplet size of the dispersed phase of CE and CE EO (**I**), mean size and PDI (**II**), and \(\zeta\)-potential distribution (**III**) of NLC and NLC EO after centrifugation (**a**) or accelerating aging (**b**) and during storage at 4 \({}^{\circ}\)C for 90 days (**c**). Mean size or \(\zeta\)-potential of CE, NLC (**III**) and CE EO, NLC EO (**III**) and uniformity, PDI, or width of NLC (yellow line), CE and CE EO, NLC EO (orange line). and trans-linaly oxide (furanoid) were incorporated only in NLC EO and not in CE EO. However, methyl clavicol, \(\alpha\)-terpineol, and eugenol were present only in the CE EO one day after preparation (Table 2). \begin{table}
\begin{tabular}{c c c c c c c} \hline \hline
**Peak** & **Ingredient** & **MW** & **AI** & **EO (mg/mL)** & **Percentage** & **CE EO** & **NLC EO** \\  & & & & & **(\%)** & **(mg/mL)** & **(mg/mL)** \\ \hline
1 & \(\alpha\)-Pinene & 136 & 931 & 24.49 & 2.41 & & \\
2 & Myrcene & 136 & 991 & 2.41 & 0.16 & & \\
3 & Cymene & 134 & 1021 & 13.94 & 1.34 & n.d. & 0.09 \(\pm\) 0.01 \\
4 & Eucalypt (1,8-cineole) & 154 & 1027 & 201.06 & 20.40 & 3.94 \(\pm\) 0.1 & 4.32 \(\pm\) 0.02 \\
5 & \(\gamma\)-Terpinene & 136 & 1052 & 1.94 & 0.11 & & \\  & cis-Linaly oxide & & 170 & 1067 & 10.27 & 0.96 & & \\  & (furanoid) & & & & & & \\
8 & Linalool & 154 & 1102 & 125.63 & 12.72 & 3.31 \(\pm\) 0.02 & 2.14 \(\pm\) 0.03 \\
9 & Dihydro-linalool & 156 & 1131 & n.q. & & & \\
10 & Camphor & 152 & 1138 & 27.28 & 2.70 & 0.44 \(\pm\) 0.04 & 0.34 \(\pm\) 0.04 \\
11 & Isoborneol & 154 & 1150 & 4.80 & 0.41 & & \\
12 & Borneool & 154 & 1159 & 14.15 & 1.36 & n.d. & 0.19 \(\pm\) 0.02 \\  & cis-Linaly oxide & & 170 & 1164 & 1.87 & 0.11 & & \\
13 & (pyranoid) & & & & & & \\
14 & Terpine-4-ol & 154 & 1171 & 3.80 & 0.30 & & \\
15 & \(\alpha\)-Terpineol & 154 & 1186 & 5.61 & 0.49 & 0.12 \(\pm\) 0.02 & n.d. \\
16 & (E)-2-Hexenyl butanoate & 170 & 1190 & 6.82 & 0.61 & & \\
17 & Methyl clavicol & 148 & 1196 & 54.76 & 5.50 & 0.08 \(\pm\) 0.01 & n.d. \\
18 & 3-(Methylthio)-1- & & & & & & \\ hexanol & & 148 & 1217 & 61.55 & 6.19 & & \\
19 & Citronellol & 156 & 1228 & 60.16 & 6.05 & & \\
20 & Isopulegol & 154 & 1233 & n.q. & & & \\
21 & Neral & 152 & 1237 & 2.26 & 0.15 & & \\
22 & Geraniol & 154 & 1253 & 107.67 & 10.89 & & \\
23 & Linalyl acetate & 196 & 1255 & 58.64 & 5.89 & 0.31 \(\pm\) 0.01 & 0.56 \(\pm\) 0.06 \\
24 & Gerani & 152 & 1267 & 4.96 & 0.42 & & \\
25 & Dihydro-linaly acetate & 198 & 1271 & n.q. & & & \\
26 & Citronellyl formate & 184 & 1273 & 3.96 & 0.32 & & \\
27 & Lavandulyl acetate & 196 & 1290 & n.q. & & & \\
28 & Geranyl formate & 182 & 1300 & 2.30 & 0.15 & & \\
29 & Eugenol & 164 & 1354 & 161.27 & 16.35 & 0.37 \(\pm\) 0.03 & n.d. \\
30 & \(\alpha\)-Ylangene & 204 & 1365 & n.q. & & & \\
31 & Geranyl acetate & 196 & 1382 & 2.56 & 0.18 & & \\
32 & Caryophyllene & 204 & 1407 & 10.75 & 1.01 & & \\
33 & Lavandulyl & & & & & \\  & isobutanoate & & & & & \\
34 & trans-\(\alpha\)-Bergamotene & 204 & 1430 & 3.56 & 0.28 & & \\
35 & \(\alpha\)-Mumulene & 204 & 1443 & 1.97 & 0.12 & & \\
36 & \(\gamma\)-Cadinene & 204 & 1503 & n.q. & & & \\
37 & Eugenol acetate & 206 & 1528 & 8.78 & 0.81 & & \\
38 & Caryophyllene oxide & 220 & 1573 & 7.32 & 0.66 & & \\
39 & epi-\(\alpha\)-Cadinol & 222 & 1639 & n.q. & & & \\ \hline  & Total identified & & & & 68.58 & & \\ \hline  & \multicolumn{4}{c}{Concentration of total EO incorporated} & & 8.57 \(\pm\) 0.15 & 7.75 \(\pm\) 0.05 \\ \multicolumn{4}{c}{n.d.: not detected; n.q: non-quantifiable.} \\ \end{tabular}
\end{table}