## 3 Results and Discussion
### General Characteristics of De Novo Bacidia rubella Genome
\begin{table}
\begin{tabular}{c c c c c c c c c c c c c} \hline \hline
**Species** & **Species** & **Genome** & **No of** & **Type I** & **Type I** & **Type I** & **PR-PKS** & **Hybrid** & **NRPS/** & **Strative** & **Metabolites** \\
**(NCBI)** & **Tag** & **(Mb)** & **Clusters** & **(Total)** & **NR-PKS** & **R-PKS** & **R-PKS** & **NRPS** & **NRPS** & **NRPS/** & **Retapolites** \\ \hline _Electoria sarmmetosa_ (ASM973377v1) & \multirow{2}{*}{alesarpred} & 39.5 & 36 & 12 & 5 & 7 & 0 & 1 & 2/12 & \begin{tabular}{c} Usnic acid, \\ electronic acid \\ (major), mammic, \\ squamatic and \\ \end{tabular} \\ \hline _Socialia gigantensis_ (ASM1945646v1) & \multirow{2}{*}{bagigpred} & 33.1 & 31 & 11 & 7 & 4 & 0 & 1 & 5/10 & Homosekikae acid \\ \hline _Socialia rheella_ (this study) & \multirow{2}{*}{benchpred} & 33.5 & 31 & 10 & 6 & 4 & 0 & 0 & 3/7 & **Atranorin** \\ \hline _Cladonia sraj_ (QGI: Ggr/DA2 myc/ ss v2.0) & \multirow{2}{*}{clagrapred} & 34.4 & 48 & 21 & 8 & 12 & 1 & 0 & 2/11 & \begin{tabular}{c} 4-O- \\ \end{tabular} \\ \hline _Cladonia magictan_ (Clmac\_v1) & \multirow{2}{*}{clamacpred} & 36.8 & 55 & 25 & 15 & 10 & 0 & 2 & 3/9 & \begin{tabular}{c} Thammolic acid, \\ batribatic acid, \\ digdymic acid, \\ squamatic acid, \\ \end{tabular} \\ \hline _Cladonia metacomilifera_ (KolR1002260\_v2) & \multirow{2}{*}{clametpred} & 36.6 & 51 & 24 & 10 & 14 & 0 & 1 & 1/11 & \begin{tabular}{c} Usnic acid, digdymic acid, \\ acid, squamatic acid, \\ rhododcalonic acid \\ \end{tabular} \\ \hline _Cladonia magictan_ (Clmac\_v1) & \multirow{2}{*}{clamacpred} & 34.5 & 68 & 34 & 14 & 20 & 0 & 2 & 3/12 & \begin{tabular}{c} **Atranorin**, \\ protocetratric acid, \\ fumaraproducteric acid \\ acid \\ \end{tabular} \\ \hline _Cladonia uncialis_ (ASM292778v1) & \multirow{2}{*}{clauncpred} & 30.7 & 61 & 30 & 15 & 14 & 1 & 2 & 1/11 & Usnic acid, \\ \hline _Cynamorella_ (Astra) & \multirow{2}{*}{cyanstpred} & 28.6 & 35 & 11 & 5 & 5 & 1 & 3/10 & Astin, skyrmion \\ \hline _Dibicis hneomyces_ (JGI) & \multirow{2}{*}{dibbaepred} & 34.2 & 55 & 27 & 11 & 15 & 1 & 0 & 4/14 & \begin{tabular}{c} Baeomycin acid, \\ squamatic acid \\ \end{tabular} \\ \hline _Endocarpp_ & \multirow{2}{*}{endpuspred} & \multirow{2}{*}{36.2} & \multirow{2}{*}{32} & \multirow{2}{*}{14} & \multirow{2}{*}{4} & \multirow{2}{*}{9} & \multirow{2}{*}{1} & \multirow{2}{*}{1} & \multirow{2}{*}{3/4} & Not reported \\ \cline{1-1} _(ZW7020)_ & & & & & & & & & & & \\ \hline _Everria prannstri_ (ASM18436v1) & \multirow{2}{*}{everypred} & 40.2 & 86 & 36 & 16 & 19 & 1 & 4 & 3/20 & \begin{tabular}{c} Usnic acid, \\ **atranorin**, and \\ chlorotransorin, \\ everine acid \\ \end{tabular} \\ \hline _Graphis scripta_ (JGI: Cbs 132367) & \multirow{2}{*}{grasscprred} & 34.8 & 54 & 21 & 6 & 15 & 0 & 1 & 6/14 & Not reported \\ \hline _Gquellecchia_ & \multirow{2}{*}{grawihescens_ (KolR1002931)} & \multirow{2}{*}{gyafalpred} & 34.4 & 41 & 16 & 8 & 8 & 0 & 1 & 3/8 & \begin{tabular}{c} Parieitin, emodin, \\ fallacinal, fraglin \\ \end{tabular} \\ \hline _Lasilia bispatica_ (ASM325442v1) & \multirow{2}{*}{lashispred} & 39.7 & 28 & 15 & 8 & 6 & 1 & 1 & 0/3 & \begin{tabular}{c} Gyrophoric acid, \\ technicor acid, \\ sublificar \\ \end{tabular} \\ \hline _Lasilia pustistata_ (ASM63619v1) & \multirow{2}{*}{laspusppred} & 32.9 & 26 & 17 & 9 & 7 & 1 & 0 & 0/4 & \
\begin{tabular}{c} Gyrophoric acid, \\ licenoric acid, \\ hiasenic acid, skyrmion \\ \end{tabular} \\ \hline _Letharia cumbiana_ (Lecol\_v1.0) & \multirow{2}{*}{letoclpred} & 52.2 & 43 & 14 & 7 & 7 & 0 & 2 & 3/6 &
\begin{tabular}{c} Vulpinic acid and \\ **atranorin** \\ \end{tabular} \\ \hline \hline \end{tabular}
\end{table} 
Table 2: Overview of biosynthetic gene clusters and polyketide synthase families found in the twenty-three studied fungal genomes. The occurrence of the major secondary substance of _B. rubella_, atranorin, is highlighted. All sequences except the de novo sequenced _B. rubella_ genome are from NCBI, if not otherwise specified in the first column. The two species _Ramulina internedia_ and _R.