###### Abstract  
Since plant's edible parts are one of the most important sources of nutrition, agronomic biofortification plays a huge role in overcoming mineral deficiency worldwide. The field-based research trial was set up in 2 years (2020 and 2021) with seven different treatments of foliar Zn and Se biofortification: 1. control (without Se or Zn solutions); 2. Se_1 treatment: 10 g/ha Se; 3. Se_2 treatment: 20 g/ha Se; 4. Se_3 treatment: 30 g/ha Se; 5. Zn_1 treatment: 3 kg/ha Zn; 6. Zn_2 treatment: 6 kg/ha Zn; 7. Se_3 Zn_2 treatment: 30 g/ha Se + 6 kg ha Zn. There were six soybean varieties of the 00 to I maturity group (Ika, Korana, Lucija, Sonja, Sunee, and Toma) included in the study, which originated from the Agricultural Institute Osjek, Croatia. After sampling the plants at the harvest, the macro- and micronutrient status in the grain, pods, leaves, and stems were determined, as well as nutrient removal by the plant. In general, biofortification treatment has a very significant influence (\(p<0.001\)) on both Zn and Se accumulation in soybean grain and the removal of the elements within all above-ground organs. The highest increments of Zn in the soybean grain were determined at the Zn_2 treatment, which was 43% higher than the control treatment. The Toma variety accumulates the highest Zn in the grain (61.47 mg/kg), and the Lucija variety accumulates the highest Se (1070.71 ug/kg). The Se content in the soybean grain was the highest at the Se_3 treatment, where it was 53 times higher compared to the control. The linear regression showed that for each kg Zn and g Se applied, the grain status increased by 3.18 mg/kg and 338.71 ug/kg, respectively. The highest Zn nutrient use efficiency (NUE) of foliar biofortification for grain (2.6%) and vegetative mass (4.4%) was with 3 kg/ha (Zn_1). Generally, for all the Se treatments, it was found that the seed and vegetative mass yields of 4.0 t/ha have average Se NUE, around 38%, and vegetative mass of around 6%. grain; yield; mineral composition; removal minerals; leaf; NUE 7 November 202428 November 282 November crucial for metabolism and as the catalytic activity of enzymes which has a role in most metabolic pathways [6]. For young children pregnant women and infants, Zn is especially severe for healthy growth. The Zn deficiency leads to anomie, alopecia, delayed growth, loss of appetite, and reproductive problems [7]. In the human body, Se is important for the reproductive system, DNA formation, and general body health. The lack of Se in the human body can lead to Keshan disease (a type of heart disease), Kashin-Beck disease (a type of arthritis), male infertility, swelling, and the loss of motion in joints, but also weakens the immune system [8]. Plants are one of the most important sources of nutrition for humans and animals. A potentially sustainable and cost-effective way to alleviate the hidden hunger worldwide is the agronomic biofortification of micronutrients and their bioavailability in edible plant parts [8; 9; 10; 11]. Usually, biofortification includes determining the dose and timing of the foliar application of Zn, Fe, Se, and I [12]. The biofortification interaction of Se and Zn has been the focus of many researchers recently [4; 13]. Except for the biofortification in the later growing stages, seed priming with Se and Zn recently showed different accumulations of these elements into the plant tissue. Rice seed priming with Se (0, 30, and 60 umol/L) increases the emergence index and total germination, as well as shoot and root lengths and dry weights [14]. Soaking seed, as a sowing pre-treatment, also leads to improving seed germination and a better establishment of seedlings [15]. The usual content of Zn in soils is 6-28 mg kg\({}^{-1}\)[16], while Se in the soil is in the range of 0.1-2 mg kg\({}^{-1}\)[17]. In Croatia, Se content in the soil is low and it varies from 0.024 to 9.45 mg Se kg\({}^{-1}\) soil [13]. Plants receive selenium from the soil, water, or atmosphere through the roots and leaf surfaces.