## 3 Results
### Frozen Storage  
Given the need for these samples to serve as canine training aids, an important variable to consider is the odor persistence of detected volatiles under storage conditions. Hence, a chemical characterization of the SLF eggs and crickets under frozen conditions was made over a 6 week period to monitor changes to the detected odor profile. Figure 4 depicts a colored odor chart highlighting the primary odor volatile (i.e., odors appearing in all replicates) array for each specimen across the 6-week study period. The crickets depicted a more abundant odor profile measured by peak area response overall from week 0 to week 5. The highest relative peak area in the crickets was seen in week 0, with the value reaching 869,768,225, and the lowest relative peak area was seen in week 5, with the value reaching 441,360,932. A total of thirteen primary compounds were detected in the cricket samples at the different sampling times: dodecane, 1-octen-3-ol, 6-methyl-2-heptanone, 5-ethyldihydro-2-(3H)-Furanone, 2-decanone, benzaldehyde, 2-butyl-2-enal, 2-butyl-2-octenal, 2-Heptanone, 3,5-Octadien-2-ol, octanal, 2-pently-pyridine, and p-xylene. Of these compounds, four were present in all 6 weeks: 1-octen-3-ol, 2-decanone, 2-butyl-hept-2-enal, and 2-butyl-2-octenal. Compared to the crickets, the SLF eggs had a much more limited odor profile overall from week 0 to week 5. The highest relative peak area in the SLF eggs was seen in week 2, with the value reaching 448,212,236, and the lowest relative peak area was seen in week 4, with the value reaching 121,828,007. A total of eight primary compounds were detected in the egg samples at different times: anisole, tridecane, dodecane, 2-nonanone, 3-octanone, D-limonene, 2,3-butanediol, and phenylethyl alcohol. Of these compounds, four were present in all 6 weeks: anisole, tridecane, dodecane, and 3-octanone. Dodecane was the only compound shared between the SLF eggs and crickets; however, it was more prevalent in the egg samples, as it was present in all 6 weeks, compared to the crickets, where it was present in 1 week. A total VOC accumulation from each specimen across the 6-week period can be seen in Figure 5. The average VOC accumulation for the SLF eggs was 278,706,213, and the average VOC accumulation for the crickets was 646,101,011. Comparing both insect samples, the crickets have more VOC accumulation across the board than the SLF eggs, with the greatest amount seen in week 0, with a value of 869,768,225. The greatest amount in the SLF eggs can be seen in week 2, with a value of 448,212,236. The VOC accumulation of both the SLF eggs and crickets stays within detection response the entire time, with the biggest decrease occurring in the eggs from week 3 to week 4. Figure 3: Venn diagram listing the primary and shared compounds from the SLF eggs, crickets, and bark. A calibration curve was created so the peak area response could be correlated to the amount of dodecane and anisole extracted across the egg and cricket samples in the frozen storage study. Using these two compounds, liquid solutions of various concentrations (5-100 ppm) were used to create external calibration curves for quantitation purposes. This was performed by injecting the range of liquid concentration solutions into the GC/MS system using the same method used for headspace sampling. An average response factor was then obtained and used to approximate the amount of VOCs being extracted by the  
Figure 4: Stacked bar graph representing the relative peak area response of the odor profiles of the SLF eggs and crickets over a 6-week sampling period. Figure 5: Total VOC accumulation graph from each specimen, SLF eggs and crickets, across the 6-week sampling period. Error bars indicate the standard error of the mean. SPME. These converted peak area responses to parts per million can be seen in Figure 6. As can be observed in the figure, anisole is present in a larger concentration across the egg samples, while dodecane is present in a larger concentration in the cricket samples.