## 4 Discussion
\begin{table}
\begin{tabular}{c c c c c c c} \hline
**Day** & **CON** & **GUA** & **WBV-E** & **GUA+WBV-E** & \(p\) & \(\varepsilon^{2}\) \\ \hline
1–10 & 2.00 \(\pm\) 0.00 & 4.00 \(\pm\) 0.00 *** & 3.00 \(\pm\) 1.00 * & 4.00 \(\pm\) 0.75 ** & 0.0005 & 0.4566 \\
11–20 & 2.00 \(\pm\) 0.00 & 4.00 \(\pm\) 0.00 *** & 2.00 \(\pm\) 0.00 ** & 4.00 \(\pm\) 0.00 *** & \(<\)0.0001 & 0.6272 \\
21–30 & 2.00 \(\pm\) 0.00 & 4.00 \(\pm\) 0.00 *** & 2.00 \(\pm\) 0.00 ** & 4.00 \(\pm\) 1.00 *** & \(<\)0.0001 & 0.6527 \\
31–40 & 2.00 \(\pm\) 0.00 & 4.00 \(\pm\) 0.75 *** & 2.00 \(\pm\) 0.00 ** & 3.00 \(\pm\) 1.00 ***++ & \(<\)0.0001 & 0.7726 \\ Total (1–40) & 2.00 \(\pm\) 0.00 & 4.00 \(\pm\) 0.25 *** & 2.00 \(\pm\) 0.00 ** & 4.00 \(\pm\) 1.00 ***++ & \(<\)0.0001 & 0.6016 \\ \hline \end{tabular}
* CON, control; GUA, group treated with guarana; WBV-E, group exposed to mechanical vibration generated in platform; GUA + WBV-E, group treated with guarana and exposed to mechanical vibration. Values are presented in median \(\pm\) IQR and \(\varepsilon^{2}\), epsilon squared. p values (Student–Newman–Keus correction) were considered significant at ** \(p<0.01\), and ** \(p<0.001\) compared with the CON group, and \({}^{\#}\)\(p<0.05\), ** \(p<0.01\), and ** \(p<0.001\) compared with the GWA group, \({}^{+}\)\(p<0.05\), \({}^{++}\)\(p<0.01\), and \({}^{+++}\)\(p<0.001\) compared with the WBV-E group.  
\end{table} 
Table 5: Stool consistency of animals submitted to some interventions. however, [PERSON] et al. [39] described, in humans, the influence of WBV on bone scintigraphy using \({}^{99\text{m}}\)Tc-methylene diphosphate. For a period of 6-weeks (40 days), the interventions with WBV (50 Hz), the WBV-E and GUA + WBV-E groups, had increased calcium levels as compared with the GUA group (Table 2). It is well-known that the calcium level in blood is mainly controlled by the parathyroid glands and, when the body needs calcium, the parathyroid glands secrete a hormone called parathyroid hormone (PTH) [40,41]. Therefore, it is possible to infer that WBV exercises promote an osteogenic effect that causes the PTH to signal the bone to release more calcium, which explains the increased calcium levels in the blood of these animals, and offers insight into on how bone construction mechanisms behave while receiving the vibration stimuli [41,42]. Considering the food intake for the period of 6-weeks, an increase of food intake was observed in the WBV-E group. This finding corroborates with [PERSON] et al. [15] and [PERSON] et al. [33] who also observed, for 6-weeks, a significant increase of food intake in rats exposed to WBV at 50 Hz, as well as an increase in a GUA group. Our finding is in agreement with [PERSON] et al. [43] who observed, after 30 days of treatment, that the daily administration of 200 and 400 mg of guarana increased appetite. In contrast, they observed a decrease in food intake of the rats exposed to WBV exercise in combination with guarana (the GUA + WBV-E group), notably, an interesting finding. It was expected that food intake would increase in the concomitant treatment, as noted in the results of both agents separately as compared with the CON group (Figure 4). Other than this decrease, no significant change in body mass of the GUA + WBV-E group was observed. Some studies have shown that guarana can slow and inhibit the production of fat cells to help decrease body fat [43,44]. With respect to the body mass of the animals (Table 4), an increase in the percentage was shown over the weeks, but no statistical difference was observed among the groups. Therefore, the body mass and food intake outcomes observed in this investigation can be highlighted as interesting findings. In healthy rats, the food intake increase did not have a direct influence on the body mass increase. In similar conditions, previous works [15,33] have shown (either in animals that only received the natural product or WBV, or in association) that the food intake increased without modification of the body mass.