## 4 Discussion
### Room Temperature  
The final objective of this study was to monitor the persistence of volatiles from the SLF eggs and crickets over a 6-week longevity period when stored in an open or closed vial at room temperature. A total of 5 primary compounds were detected in the open egg samples: anisole, D-limonene, 2,6-bis(1,1-dimethylethyl)-4-(1-methylpropyl)-phenol, 6,6-dimethyl-2-methylene-(1S)-bicyclo[3.1.1]heptane, and (E)-2-ocrenal. A total of 12 primary compounds were detected in the open cricket samples: 2,6-bis(1,1 dimethylethyl)-4-(1-methylpropyl)-phenol, hexanal, 1-octen-3-ol, 2-pentyl-furan, 2-butylhept-2-enal, 2-butyl-2-ocrenal, 5-ethyldihydro-2(3H)-furanone, octanal, 3-ethyl-2,5-dimethyl-pyrazine, nonanal, (1R)-2,6,6-Trimethylbicyclo[3.1.1]hept-2-ene, and decanal. A total of seven primary compounds were detected in the closed egg samples: anisole, D-limonene, ethyl ester hexanoic acid, butanoic acid, ethyl ester butanoic acid, ethyl ester octanoic acid, and phenylethyl alcohol. A total of 14 primary compounds were detected in the closed cricket samples: hexanal, 1-octen-3-ol, 2-butylhept-2-enal, 2-butyl-2-ocrenal, 2-n-butyllacrolein, 6-methyl-2-heptanone, benzaldehyde, cis-4-decene, (E)-4-undecene, 2-propyl-2-heptenal, 5-ethyldihydro-2(3H)-furanone, octanal, nonanal, and heptanal. In both the SLF eggs samples and the cricket samples, primary compounds were detected that were not present in the frozen study. This is most likely because half of the samples were left open, and these compounds could have come from the surrounding environment and are not an accurate representation of the VOC profile of either the SLF eggs or crickets and represent environmental contamination. These compounds include 2,6-bis(1,1 dimethylethyl)-4-(1-methylpropyl)-phenol, 6,6-dimethyl-2-methylene-(1S)-bicyclo[3.1.1]heptane, 3-ethyl-2,5-dimethyl-pyrazine, (E)-2-ocrenal, (1R)-2,6,6-trimethylbicyclo[3.1.1]hept-2-ene, and decanal. All these listed compounds were present in the open egg or cricket samples; therefore, it can be assumed that these were a product of environmental contamination. Regarding the overall persistence of each specimen type, both the SLF eggs and crickets depicted a weekly change in the reported VOC profile. PCA analysis corroborated that the primary odor set of compounds did not cluster as a function of the week, indicating continuous flux over time. The exceptions can be seen in the open SLF egg and cricket samples as weeks 1 through 4 of the eggs are clustered, and weeks 3 and 4 of the crickets are clustered. This would indicate that between these weeks, the VOC profile of each specimen type remained stable. The other exception can be seen in the closed SLF egg samples as weeks 0 through 3 and 5 are clustered, indicating the VOC profile in between these weeks remained stable in the SLF eggs only. The VOC abundance of the crickets, both open and closed, held somewhat until week 3 when a steep decline was seen. The VOC abundance of the eggs, both open and closed, was relatively low throughout the 6-week period. However, there was an increase in VOC abundance in the closed eggs in week 3, and this trend persisted until the last week of the sampling period. It was also at week 3 where the VOC abundance of the closed eggs, open crickets, and closed crickets began to equal out, and this trend also persisted until the last week of the sampling period. This trend was also depicted in the one-way ANOVA measuring the effect of dissipation type (closed or open lid) on VOC accumulation. In the SLF egg samples, a \(p\)-value less than 0.05 was produced (\(p\) = 0.0005), indicating a statistically significant effect on VOC accumulation as a function of lid type. This analysis reveals that it is more beneficial for the SLF egg samples to be stored with a closed-lid vial containment system between sampling times, as leaving the vials open would significantly affect the VOC accumulation detected in the samples. For the cricket samples, the one-way ANOVA test was run to analyze the VOC accumulation in relation to lid type, as well.