## 3 Results  
This research was experimental and was conducted in a laboratory. The dependent variable was peroxide residue at 1 h, 24 h, and 48 h, while _Trigona_ spp. propolis 10% and Sodium Ascorbate 10% + Tween 80 0.2% were the independent variables. The Shapiro-Wilk normality test gave a \(p\)-value \(>0.05\), suggesting that the data were normally distributed. Figure 1 presents the average dissolved oxygen (ppm) results. The statistical test, a one-way ANOVA, found a \(p\)-value \(<0.05\) in both antioxidant groups, i.e., _Trigona_ spp. propolis 10% and sodium ascorbate combined with Tween 80 0.2%. These results indicated a significant difference in peroxide residue between _Trigona_ spp. propolis 10% and sodium ascorbate combined with Tween 80 0.2% in the application period. Table 1 shows the results of different advanced tests that we used to analyze the results that showed the effects of different application duration times, i.e., 1 h, 24 h, and 48 h, in each antioxidant group. In the _Trigona_ spp. propolis 10% antioxidant group, we found a significant difference in peroxide residue between 1 h and 24 h that was 1320 ppm (\(p=0.000\); \(p<0.05\)). The results also showed that there was a significant difference (\(p=0.000\), \(p<0.05\)) of 1020 ppm between 24 h and 48 h. There was a difference between 1 h and 48 h (\(p=0.000\), \(p<0.05\)) as well. In sodium ascorbate 10% combined with Tween 80 0.2%, a significant difference (\(p=0.000\), \(p<0.05\)) was found between 1 h and 24 h, which was 1940 ppm. Between 24 h and 48 h, there was a significant residual difference (\(p=0.000\), \(p<0.05\)) of 0.876 ppm. There was also a significant difference between 1 h and 48 h (\(p=0.000\), \(p<0.05\)). Figure 2 shows significant differences in peroxide residues among the _Trigona_ spp. propolisis 10% group, sodium ascorbate 10% combined with Tween 80 0.2% group, and control group (\(p\) = 0.000; \(p\) <0.05) for each application duration time. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Antioxidant** & \begin{tabular}{c} **The Length of** \\ **Application Time** \\ \end{tabular} & **Comparison** & \begin{tabular}{c} **Mean** \\ **Difference** \\ \end{tabular} & \
\begin{tabular}{c} **95\% CI** \\ **(Min-Max)** \\ \end{tabular} &
\begin{tabular}{c} **\(p\)-Value** \\ **\end{tabular} \\ \hline \multirow{3}{*}{_Trigona_ spp. propolisis 10\%} & 1 h & 24 h & 1.320 & 0.832–1.807 & 0.000 * \\  & 48 h & 2.340 & 1.852–2.827 & 0.000 * \\ \cline{2-6}  & 24 h & 48 h & 1.020 & 0.532–1.507 & 0.000 * \\ \cline{2-6}  & 1 h & 24 h & 1.940 & 1.478–2.401 & 0.000 * \\ \cline{2-6}  & & 48 h & 2.816 & 2.354–3.277 & 0.000 * \\ \cline{2-6}  & 24 h & 48 h & 0.876 & 0.414–1.337 & 0.001 * \\ \hline \hline \end{tabular}  
* Post hoc test: Tukey’s HSD test; \(p\) < 0.05: significant.  
\end{table} 
Table 1: The test results for application duration times of 1 h, 24 h, and 48 h in each antioxidant group. Figure 1: The average distribution of dissolved oxygen based on duration of application time in each antioxidant group. (The difference between the average dissolved oxygen of each antioxidant group during application time was statistically significant at 0.05 level.) To see further group differences, a post hoc test was conducted. Table 2 shows the results of the advanced difference test between the _Trigona_ spp. propolis 10% group, sodium ascorbate 10% combined with Tween 80 0.2% group, and control group with different application times of 1 h, 24 h, and 48 h. There was a significant difference between _Trigona_ spp. propolis 10% and the control group. The same result was also found between the sodium ascorbate 10% combined with Tween 80 0.2% group and the control group. However, no significant difference was found between _Trigona_ spp. propolis 10% and sodium ascorbate 10% combined with Tween 80 0.2%.