## 2 Results  
### Chemical Profiling  
The total flavonoid compound concentration for S. nigrum roots and aerial parts was determined as 12.49 \(\pm\) 0.17 mg QE/g dry extract and 15.73 \(\pm\) 0.22 mg QE/g dry extract, respectively. Regarding the detailed analysis based on research by [PERSON] et al. (2013) [32], twenty-four compounds that belonged to flavonoids (Table 1), steroidal glycosides, steroidal glycocalkaloids or steroidal alkaloids groups (Table 2) were searched for extracts, and nineteen of them were found in at least one sample. Among flavonoids, delphinidin-3-O-(6''-O-\(\alpha\)-rahmopyranosyl)-\(\beta\)-glucopyranoside, isoquercitrin, naringenin and pelargonidin-3-O-(6''-O-\(\alpha\)-rahmopyranosyl)-\(\beta\)-glucopyranoside were found, both in aerial parts and root extract; while among steroidal glycosides, steroidal glycocalkaloids or steroidal alkaloids solamargine, solanine, solasodine, solasodine and tomatine were found in both extracts (source chromatograms are given as Supplementary Files).  
\begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**No.** & **Compound** & **_m/z_** [M + H]\({}^{*}\) & **RT [min]** & **Aerial Parts** & **Roots** \\ \hline
1. & Astragalain & 449.2 & - & nd 1 & nd \\ \hline
2. & Cyandin–3-O-(6′′-O-\(\alpha\)-rahmopyranosyl)-\(\beta\)-glucopyranoside & 595.0 & 5.78 & 0.82 \(\pm\) 0.11 2 & nd \\ \hline
3. & Delphinidin-3-O-(6′′-O-\(\alpha\)-rahmopyranosyl)-\(\beta\)-glucopyranoside & 611.0 & 7.32 & 22.62 \(\pm\) 0.16 & 5.51 \(\pm\) 0.13 \\ \hline
4. & Eriodicylol & 289.0 & 5.83 & 2.17 \(\pm\) 0.09 & nd \\ \hline
5. & Isoquercitrin & 465.1 & 8.85 & 7.74 \(\pm\) 0.16 & 6.31 \(\pm\) 0.15 \\ \hline
6. & Kaempfer-3-O-rutinoside & 595.1 & 6.81 & 2.72 \(\pm\) 0.11 & 3.66 \(\pm\) 0.12 \\ \hline
7. & Kaempfer-3-O-rutinoside-7-O-glucose & 757.0 & 6.64 & 0.85 \(\pm\) 0.12 & 2.87 \(\pm\) 0.10 \\ \hline
8. & Linalool-3-O-glucopyrananoyl-(1–6)-arabinopyranoside & 449.2 & - & nd & nd \\ \hline
9. & Naringenin & 273.0 & 7.10 & 3.54 \(\pm\) 0.20 & 14.14 \(\pm\) 0.19 \\ \hline
10. & Naringenin chalcone-glucose & 435.0 & 7.02 & nd & 3.77 \(\pm\) 0.04 \\ \hline
11. & Naringenin-7-O-glucose & 435.0 & 7.02 & nd & 3.69 \(\pm\) 0.08 \\ \hline
12. & Pelargonidin-3-O-(6′′-O-\(\alpha\)-rahmopyranosyl)-\(\beta\)-glucopyranoside & 579.0 & 7.32 & 34.39 \(\pm\) 0.30 & 60.05 \(\pm\) 0.21 \\ \hline
13. & Querecetine-3-O-gentiboiside & 627.0 & 7.30 & 2.84 \(\pm\) 0.05 & nd \\ \hline
14. & Rutin & 611.0 & 7.33 & 22.54 \(\pm\) 0.15 & nd \\ \hline \hline \end{tabular} \({}^{1}\) nd—not detected; 2 percentage based on the peak area normalization.  
\end{table}
Table 1: _S. nigrum_ methanolic extracts flavonoids content (%).  
\begin{table}
\begin{tabular}{c c c c c} \hline \hline
**No.** & **Compound** & **_m/z_ [M + H]\({}^{*}\)** & **RT [min]** & **Aerial Parts** & **Roots** \\ \hline
1. & Chaconine & 853.5 & - & nd 1 & nd \\ \hline
2. & Demissine & 1019.2 & 6.95 & nd & ++ 2 \\ \hline
3. & Solamargine & 868.4 & 6.34 & + & + \\ \hline
4. & Solandidienol chacotriose & 900.3 & - & nd & nd \\ \hline
5. & Solandidienol solatriose & 916.3 & - & nd & nd \\ \hline
6. & Solanidine & 398.5 & 9.74 & + & + \\ \hline \hline \end{tabular} \({}^{1}\) nd—not detected; 2 percentage based on the peak area normalization.  
\end{table}
Table 2: S. nigrum methanolic extracts steroidal glycosides, steroidal glycoalkaloids and steroidal alkaloids’ profile.