# Basic Bioelement Contents in Anaerobic Intestinal Sulfate-Reducing Bacteria
## 3 Results
\begin{table}
\begin{tabular}{c c c c c c c c c c c c} \hline \hline
**Groups** & **Parameters** & **Na\({}^{+}\)** & **K\({}^{+}\)** & **Ca\({}^{2+}\)** & **Mg\({}^{2+}\)** & **Zn\({}^{2+}\)** & **Fe\({}^{2+}\)** & **Cu\({}^{+}\)** & **Mn\({}^{2+}\)** & **Co\({}^{2+}\)** & **Ni\({}^{2+}\)** & **Mo\({}^{2+}\)** \\ \hline \multirow{3}{*}{CD} & X & **4.46** & **21.84** & **15.66** & **1.07** & **0.17** & **3.96** & **0.02** & **0.033** & **0.0005** & **0** & **0.0011** \\  & **Ax (t lim 2.26, n = 9)** & 0.22 & **3.17** & **13.31** & 0.24 & 0.22 & **2.62** & 0.01 & 0.01 & 0.0004 & 0 & 0.0011 \\ \hline \multirow{3}{*}{UC} & X & **4.22** & **17.30** & **7.36** & **0.77** & **0.08** & **1.47** & **0.023** & **0.027** & **0.0005** & **0.0052** & **0.0001** \\  & **Ax (t lim 2.16, n = 14)** & 0.28 & 0.89 & **2.29** & 0.06 & 0.06 & 1.02 & 0.01 & 0.002 & 0.0002 & 0.0061 & 0.0003 \\ \hline \multirow{3}{*}{H} & X & **4.16** & **18.37** & **11.41** & **0.94** & **0.25** & **8.41** & **0.04** & **0.03** & **0.001** & **0.01** & **0.0022** \\  & **Ax (t lim 2.23, n = 11)** & 0.24 & 1.38 & **3.67** & 0.13 & 0.11 & **3.69** & 0.039 & 0.004 & 0.0003 & 0.0144 & 0.0010 \\ \hline \hline \end{tabular} Note: [PERSON]’s disease (CD), ulcerative colitis (UC), healthy (H), X is average mean. Inaccurate data according to Student’s test (_t_) at \(t\leq 0.05\) are in bold.  
\end{table} 
Table 2: Significance of the mean values of the metal concentrations in the cytoplasm of SRB from patients with IBD. \begin{table}
\begin{tabular}{c c c c c c} \hline \hline
**Importance** & **Factors** & **Power** & \multicolumn{4}{c}{**Principal Components**} \\ \cline{3-6}  & & & **PC1** & **PC2** & **PC3** \\ \hline
1 & Mn & 0.87 & 0.874 & 0.333 & 0.068 \\
2 & Mg & 0.86 & 0.836 & 0.388 & \(-\)0.138 \\
3 & Co & 0.86 & 0.729 & \(-\)0.456 & 0.3411 \\
4 & Ca & 0.84 & 0.718 & 0.337 & \(-\)0.468 \\
5 & Mo & 0.81 & 0.806 & \(-\)0.323 & 0.233 \\
6 & Fe & 0.78 & 0.765 & \(-\)0.317 & 0.297 \\
7 & Na & 0.77 & 0.066 & 0.460 & 0.753 \\
8 & Cu & 0.76 & 0.565 & \(-\)0.598 & \(-\)0.279 \\
9 & K & 0.72 & 0.475 & 0.701 & \(-\)0.074 \\
10 & Zn & 0.68 & 0.796 & 0.200 & \(-\)0.121 \\
11 & Ni & 0.59 & 0.296 & \(-\)0.699 & \(-\)0.110 \\ \hline \hline \multicolumn{3}{c}{Eigenvalues} & 5.02 (S) & 2.38 (S) & 1.18 \\ \hline \multicolumn{3}{c}{Total variance (\%)} & 45.65 & 21.65 & 10.74 \\ \hline \end{tabular} Note: values highlighted in red mean that the loadings of this factor are insignificant, the sign “\(-\)” determines the position of the point in the coordinate system, S is significant.  
\end{table} 
Table 3: PCA results and explained variations. contribution to the distribution of samples of SRB strains is manganese. The second major component of PC2 also has a significant eigenvalue (2.38) and explains up to 21.65% of the variations. The second component is loaded with elements such as potassium and magnesium, while negative correlations were observed for copper, nickel, and cobalt metals, although they exerted a significant load. The third component explained up to 10.74% of the variations, and sodium (power 0.77) put a significant load on it. On the plot, each variable is represented by a point in coordinates (PC1, PC2). Analyzing it similarly to the score plot, we can observe which variables are closely related and which are independent [44]. However, the most informative joint study of paired plots of scores and loadings (Figure 3). Figure 3A shows the grouping of chemical elements into two clusters, the first cluster includes elements such as manganese, magnesium, zinc, and cobalt. Sodium and calcium  
Figure 3: Loading (**A**) and score (**B**) plots of the PCA for samples from patients with IBD. Note: designation of SRB strains as in Table 1; UC indicates ulcerative colitis, CD indicates [PERSON]’s disease, and H indicates a healthy person. in this cluster are slightly separated. This is probably due to their low importance in the factor distribution (powers were 0.77 and 0.72, respectively).