# Towards Understanding the Function of Aegerolysins
## 3 Sequences, Structures, and Structural Models of Aegerolysins
Circles, proteins from fungi; A color legend is supplied for individual taxa: proteins from _Agaricomycetes_ (GlyA6 and PlyB in grey and others in brown), _Eurotiomycetes_ (in blue), _Sordariomycetes_ (in green), and _Dothidomycetes_ (in violet). Squares, bacteria; _Firmicutes_ (in red), and _Proteobacteria_ (in orange). Triangle, insects (in light green); diamond, virus (in black). Aegerolysin phylogeny (calculated by Mega [154]) is not following taxon phylogeny. Structures and AlphaFold2 calculated models of aegypti and protein partners [14]; presentation by PyMOL [62]. Aegerolysins: GlyA6/PlyA, ostreolysin A6/pleurotolysin A from _Pleurotus ostratus_, PDB ID: 6 MYJ; EryA, erylysin A, and PlyA2, pleurotolysin A2 from _Pleurotus ersyngii_; Aa-Pri1, aegerolysin Aa-Pri1 from _Cyclocyhe aegerita (Agrocybe aegerita)_; MpPRIA1 and MpPRIA2, putative aegerolysin genes from _Moniliophthora pernicosa_; GME7309, _Ligosus rhinocr ots_; aegerolysin-domain-containing protein; Asp  
HS, Asp-hemolysin, and Asp-HS-like, Asp hemolysis-like from _Nesoartoya fungiata_ (_Apergillus fungiata_); Ter, terrelysin from _A. terreus_; AoHlyA, _A. orgaze_ hemolysis; NigA1 and NigA2, nigerolysin A1 and A2 from _A. niger_; BlyA, beauveriolysin A from _Beauveria hassiana_; AgI1, _Hypocra atroviridis_ (_Trichodemma atroviridis_) aegerolysin; L152, _Alternaria gelsen_ aegerolysin; Cry34 Ab1 (Gpp34 Ab1) 13.6 kDa insecticidal crystal protein PDB ID: 4 JOX from _Bacillus thuringiensis_; Cbm17.1 and Cbm17.2, hemolysis-like protein Cbm17.1 and Cbm17.2 from _Paraclostridium bifermentans_ (_Clostridium bifermentans_); AfIP-1A, two-component insecticidal protein 16 kDa unit, PDB ID: 5V3S, from _Alcaligenes faecalis_; RahU, RahU protein, PDB ID: 6 ZC1, from _Pseudomonas aeruginosa_; P23, protein 23 from _Chrysoidevis includes_ (_Pseudoplasma includes_); and TnAV6a1 gp029; ORF029 from _Trichpolususii_ ascovirus 6a1 (2c). Partner proteins: PlyB, pleutorolysis B, PDB ID: 4 QEJ; EryB, erlypsin B; NigB1, nigerolysin B1, BlyB, beauveriolysin B; Cry35 Ab1 (Tpp35 Ab1), 43.8 kDa insecticidal crystal protein, PDB ID: 4 JP0; Cry16 Aa and Cry17 Aa, pesticidal crystal-like protein Cry16 Aa and Cry17 Aa; and AfIP-1B, two-component insecticidal protein 77 kDa unit. The position of the only three conserved glycines is marked in the structure of OlyA6 (in magenta) (Figure S1), the position of the fragment of chemical structure of SM (18:1) (in orange and marked with arrow). SM, sphingomyelin; Typical signature Y/F-G-X2-F/Y-X6-G-G for fungal MACPF domain is marked in PlyB (magenta); x, Cry16 Aa/Cry17 Aa/Cbm17.1/Cbm17.2 act together;?, no info about component B; +, having component B. For some species, all aegerolyins have been reasonably described, while others may have additional genes. One aegerolysin is encoded by the genome of _P. ostreatus_, _A. terreus_, _B. bassiana_, _T. atroviride_, _A. gelsen_, _A. faecalis_, _T. ni_ ascovirus 2c; two aegerolyins are encoded by _P. erygii_, _A. niger_, _A. fumigatus_ (except for one strain that has one aegerolysin), _P. aeruginosa_, and _C. bifermentans_; _A. oryzae_ encodes one to four and _P. includes_ four; _A. aegerita_ and _M. perriciosa_ encode six. No data for additional aegerolysin were found for _L. rhinocreus_. Five structures of aegerolyins have been solved: _P. ostreatus_**PlyA** (PDB ID: 4 OEBA) and **OlyA6** (PDB ID: 6 MYJ and some analogs), _B. thuringiensis_**Cry34 Ab1** (Gpp34 Ab1) (PDB ID: 4 JOX), _A. faecalis_**AfIP-1A** (PDB ID: 5V3S), and _P. aeruginosa_**RaU** (PDB ID: 6 ZC1). In addition, two structures of the two-component partner proteins _P. ostreatus_**PlyB** (PDB ID: 4 QEJ) and _B. thuringiensis_**Cry35 Ab1** (Tpp35 Ab1) (PDB ID: 4 JP0) have been solved, and furthermore, the membrane embedded pleutorolysis **PlyA/Plyb** pore with 13-fold symmetry (PDB ID: 4V2T) from _P. ostreatus_ (Figure 1).