## 1 Introduction  
The presence of drugs in the environment, including non-steroidal anti-inflammatory drugs (NSAIDs), has been increasingly signaled. NSAIDs are analgesics and anti-inflammatory medications, often used for mild to moderate pain. Due to the relatively small number of side effects, they are considered safe, widely used, and available without a prescription. This contributes to their high consumption and, consequently, their presence in sewage and the environment. Among others, naproxen and diclofenac have been found in European waters at concentration ranges of 3-753 ng/L and 1-429 ng/L, respectively [1, 2, 3, 4]. Concentrations observed in the environment are not the root of acute toxicity. However, it has been clearly demonstrated that they lead to chronic toxicity. It manifests itself, among others, in changes in the mating behavior of fish, damage to the gills and liver, cardiac arrhythmias, teratogenic changes, and infertility [3; 4; 5; 6]. The drug's degradation efficiency in biological wastewater treatment plants ranges from 40 to almost 100%, depending on the type of contamination, its concentration, and activated sludge properties [7; 8]. Diclofenac is one of the most difficultly degraded drugs among NSAIDs, also characterized by high toxicity [1]. Due to the incomplete degradation of NSAIDs, microorganisms supporting activated sludge and their effective implementation into the indigenous microbiome of sewage treatment plants are sought. Studies on the effect of bioaugmentation on the indigenous microbiome of sewage treatment plants and its degradation efficiency were carried out on various medicinal substances, including progesterone, where an improvement in the removal of this compound by activated sludge was demonstrated in the presence of an introduced strain of _Rhodococcus_ sp. HYW [8]. [PERSON] et al. [9] successfully introduced the _Sphingopyxis granuli_ RW412 strain into the microbiome of a wastewater treatment plant in Grenada, achieving seven times faster degradation of ibuprofen compared to the indigenous microbiome. At the same time, no accumulation of ibuprofen degradation intermediates was observed. In addition, this strain did not negatively affect the microbial population of the sewage treatment plant while maintaining its own stability [9]. Moreover, after introducing _Bacillus thuringiensis_ B1(2015b), _Stenotrophomonas maltoplilia_ KB2, and _Pseudomonas moorei_ KB4 into the activated sludge system, only a minor increase in the metabolic activity and diversity of the initial microbiome were observed. However, even though these bacteria could degrade selected NSAIDs, no significant differences were observed between the degradation rate by indigenous microbiomes and those introduced by these strains. The exception was the degradation of diclofenac, which was more effective in bioaugmented activated sludge [10]. [PERSON] et al. [11] showed that the _Stenotrophomonas maltoplilia_ KB2 strain might negatively affect the remaining strains. Therefore, effective augmentation required only the B1(2015b) and KB4 strains. Such augmented active sludge degraded diclofenac, naproxen, ibuprofen, and paracetamol more efficiently than the native microbiome. In addition, it was observed that these strains survived in bioreactor conditions in the presence of activated sludge for five weeks of the experiment [11]. Despite reports of effective augmentation of the indigenous microbiome by bacterial strains with increased degradation potential, most often, introducing such microorganisms into the microbial cultures of sewage treatment plants leads to their displacement. The reason is the changing environmental conditions in the sewage treatment plant and the competition between microorganisms. The solution to this problem is the construction of preparations based on immobilized bacteria or enzymes [12]. Laccase is often used in bioremediation processes as an enzyme with a broad substrate specificity and high oxidative potential.