_Applied_  
_sciences_  
_Article_  
**A Comparative Bio-Evaluation and Chemical Profiles**  
**of _Calendula officinalis_** L. Extracts Prepared via**  
**Different Extraction Techniques**  
**[PERSON] 1, [PERSON] 1, [PERSON] 1, [PERSON] 2,3,*, [PERSON] 3, [PERSON] 4, [PERSON] 5, [PERSON] 6, [PERSON] 7,* and [PERSON] 8, 2  
1 Department of Biology, Science Faculty, Selcuk University, 42130 Konya, Turkey;  
[EMAIL_ADDRESS] (G.A.); [EMAIL_ADDRESS] (G.Z.); [EMAIL_ADDRESS] (K.I.S.)  
2 Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam  
3 Department of Health Sciences, Faculty of Science, University of Mauritius, 230 Reduit, Mauritius;  
[EMAIL_ADDRESS]  
4 Science and Technology Research and Application Center, Dicle University, 21280 Diyarbakir, Turkey;  
[EMAIL_ADDRESS]  
5 Laboratoire de Genetique, Biochimie et Biotechnologies Vegetales GBBV, Faculte des Sciences de la Nature et de la Vie, Universite Freres Mentouri Constantine1, Route d'Ain El Bey, 25017 Constantine, Algeria;  
[EMAIL_ADDRESS]  
6 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Dicle University, 21280 Diyarbakir, Turkey;  
[EMAIL_ADDRESS]  
7 Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, via Pansini, 5, 80131 Naples, Italy  
8 Department of Pharmaceutical Sciences, Section of Food Science and Nutrition, University of Perugia, via San Costanzo 1, 06126 Perugia, Italy; [EMAIL_ADDRESS]  
* Correspondence: [EMAIL_ADDRESS] (M.F.M.); [EMAIL_ADDRESS] (M.G.)  
Received: 19 July 2020; Accepted: 24 August 2020; Published: 26 August 2020  
**Featured Application: This study provides valuable data on the influence of extraction techniques on the recovery of bioactive compounds from _Calendula officinalis_ useful for the formulation of therapeutic preparations.**  
**Abstract: _Calendula officinalis_** L., (marigold), well known for its medicinal properties, has been extensively studied for its therapeutic properties. Nonetheless, as far as the literature could establish, no study has attempted to comparatively assess the biological (antioxidant and enzyme inhibitory potential) of the flowers, leaves, and roots of _C. officinalis_ extracted using conventional (maceration and Soxhel extraction (SE)) and non-conventional extraction (homogenizer (HAE) and ultrasound (UAE) assisted extraction) techniques. The detailed phytochemical profile of each extract along with the concentration of specific bioactive compounds has also been established. Total phenolic content was highest for the flower extracts while flavonoid content was highest in the leaf extracts. Phytochemical profiling showed that the extraction method influenced the phytochemical composition of the extract. Nicotiflorin was identified in the flower extracts only while amendoflavone occurred only in the roots, inferring that the occurrence of bioactive compounds varies within a plant. The flower extracts showed highest antioxidant potential while the roots extracts were potent inhibitors of cholinesterase and tyrosinase. This study provides valuable data on the influence of extraction techniques on the recovery of bioactive compounds from plants. In an endeavor to scale-up extraction from plant considering the more efficient extraction method is of paramount importance. Moreover, the study highlighted the necessity to thoroughly examine the biological activities of various parts of a plant obtained via different extraction protocols.  
**Keywords:** marigold; maceration; cholinesterase; tyrosinase