sentence: An Echo Liquid Handler ( Labcyte , San Jose , CA , USA ) was used to transfer 200 nL of DMSO-dissolved compounds via acoustic-coupled ejection from 10 mM source plates into 384-well black , flat-bottom , black-walled microplates , for a final compound concentration of 10 μ M. Compounds were sourced from the following libraries : LOPAC ( Sigma Aldrich ) , The Spectrum Collection ( Microsource Discovery Systems ) , MEGx ( Analyticon Discovery ) , and NATx ( Analyticon Discovery ) .