sentence: 50 μ M α - syn WT ( in 50 mM Tris , pH 7.5 , 150 mM KCl , 0.05 % NaN ) monomer was incubated with the WT α - syn PFFs and G51D α - syn PFFs ( 5 mol % , equivalent to monomer concentration ) with 10 μ M ThT in the reaction mixture , separately .