sentence: For KiPIK screening , immunoblotting and immunofluorescence microscopy , rabbit polyclonal antibodies to H3T3ph ( B8634 ) , INCENP ( P240 , Cell Signaling Technology # 2807 ) , INCENP-S446ph ( Jan-Michael Peters , IMP , Vienna ) , INCENP-TSSph ( Michael Lampson , University of Pennsylvania ) , BCL9L S915ph ( Cell Signaling Technology # 13325 ) , Neurogranin S36ph ( Merck-Millipore , ABN426 ) and γ - tubulin ( AK-15 , Sigma , T3320 ) ; rabbit monoclonal antibodies to the S-pT-P motif ( D73F6 ; Cell Signaling Technology # 5243 ) , Cyclin B1 ( D5C10 ; Cell Signaling Technology # 12231 ) and Vinculin ( E1E9V ; Cell Signaling Technology # 13901 ) ; mouse monoclonal antibodies to H3S28ph ( CMA315 ) and phospho-tyrosine ( P-Tyr-100 ; Cell Signaling Technology # 9411 ) ; and sheep polyclonal antibodies to Aurora B ( SAB.1 , Stephen Taylor , University of Manchester ) and BCL9L ( R & D Systems , AF4967 ) were used .