sentence: Taken together , cross-bridge formation is reduced in disused muscle , accompanied by a change in the length dependence.Given titin 's role as a molecular ruler ( Whiting et al ., 1989 ; Trinick , 1994 ; Gregorio et al ., 1999 ) , the abnormalities in the sarcomeric structure in disused muscle ( Fig. 1 , B – D ) may be due to a change in its expression , resulting in depressed mechanical properties ( Fig. 2 , A – C ) .