sentence: Whole ‐ cell protein extracts were obtained after homogenization of ischaemic muscles of the different groups of mice in ice ‐ cold RIPA buffer ( pH = 8) containing 50 mM Tris – HCl , 150 mM NaCl , 5 mM EDTA , 1 % Triton X ‐ 100 , 0.5 % sodium deoxycholate , 0.1 % SDS with a cocktail of proteases and phosphatase inhibitors ( MiniComplete , PhosphoStop and PMSF , Roche , B â le , Switzerland ) .