sentence: A total of 5 ug of antibody against H3K27ac ( Cell Signaling Technology , D5E4 , 8173 ) , H3K4me1 ( Abcam , ab8895 ) , H3K4me3 ( Millipore , 07-473 ) or PolII ( Santa Cruz , N-20 , sc-899X , lot #C 0813 ) was added to the digested chromatin ( 1,000 ul ) and incubated overnight at 4 ° C.For the ChIP-seq of SWI / SNF complex subunits , the protocol was slightly modified .