sentence: Antibodies used for immunoblotting / immunohistochemistry were phospho-Cav-1 ( Tyr14 ) ( code 61338 , BD Biosciences , Buccinasco , Italy ) , Cav-1 ( code SC-894 , Santa Cruz Biotechnology , Dallas , USA ) , Cav-2 and Cav-3 ( code 610684 and 610420 , BD Biosciences , Buccinasco , Italy ) , phosho-Src ( Tyr418 ) ( code 569732 , Merck-Millipore , Milan , Italy ) , total and phospho-AKT ( Ser473 ) ( code # 2920 and # 4060 , Cell Signalling , Milan , Italy ) , total and phospho-ERK ( Tyr204 ) ( code SC-135900 and SC-7883 , Santa Cruz Biotechnology , Dallas , USA ) , MKI67 ( code NCL-L-Ki67-MM1 , NovocastraTM Laboratories Ltd , Newcastle Upon Tyne , United Kingdom ) , CD31 ( code SC-8306 , Santa Cruz Biotechnology , Dallas , USA ) , cleaved Caspase-3 ( code # 9661 , Cell Signalling , Milan , Italy ) and tubulin ( code T5168 , Sigma-Aldrich , Milan , Italy ) .