sentence: To assess the activation of T cells after viral infection , splenocytes from B6.CgThy1a-Tg ( TcraTcrb ) 8Rest / J ( PMEL ) mice were isolated and seeded ( 4 × 10 ) in a 96-well flat-bottomed plate coated with an anti-CD3 antibody ( clone 145-2 C11 , 100314 BioLegend ) for 24 hours .