sentence: The cDNA templates for RT-PCR and Q-RT – PCR were prepared by random priming of 2 μ g of total RNA with 200 units of Moloney Murine Leukemia Virus reverse transcriptase.Quantification of Anks3 , Anks6 , Aqp1 , Aqp2 , Aqp3 , Cep290 , Gli2 , Icam1 , Nek8 , Nphp1 , Nphp4 , p53 and Vit32 expression was carried out using the SYBRGreen technology ( Qiagen , GmbH , Hilden , Germany ) using oligonucleotides listed in S3 Table .