sentence: In this approach , 50 animals were randomly sampled among the four and three AA and SF subpopulations , respectively , to constitute two populations.Eleven microsatellite markers were used to perform the analysis ( AHT4 , AHT5 , ASB2 , HMS1 , HMS3 , HMS6 , HMS7 , HTG4 , HTG6 , HTG10 , VHL20 ) , with all but two ( HMS1 and HTG6 ) being recommended by the International Society of Animal Genetics for parentage testing and used in similar studies ( except HMS1 ) [ 7,9,10 ] .