sentence: Apart from well ‐ known gene mutations ( e.g ., TP53 , EGFR , MTOR , KRAS and SETD2 ) ,33 our analysis also identified several potential lung cancer associated gene mutations that were rarely reported ( e.g ., HOXA4 , MST1 and CD209 ) .34 , 35 , 36 , 37 When comparing with the mutation frequency of top 50 most frequently mutated genes reported in 412 LUAD cases from the TCGA cohort ,8 our cohort exhibited lower prevalence of recurrent mutations , especially in TP53 ( 27.78 % vs. 47.57 % , p = 0.024 ) , KRAS ( 11.11 % vs. 27.91 % , p = 0.030 ) , STK11 ( 0.00 % vs. 15.53 % , p = 0.005 ) and KEAP1 ( 0.00 % vs. 14.81 % , p = 0.009 ) ( Supporting Information , Table 6 ) .