sentence: Mouse monoclonal antibodies against PTPN12 ( clone 4G6 ; Abnova ) , PTPN22 ( clone 4F6 ; Abnova ) , p21 ( clone SXM30 ; BD ) , Fyn ( clone 1S ; Abcam ) , and p18 ( clone DCS118 ; Cell Signaling Technology ) were directly conjugated to DyLight650 according to the manufacturer ’ s protocols ( Thermo Fisher Scientific ) .