sentence: After washing in PBS / 10 % heat-inactivated fetal calf serum ( Sigma F7524 ) and erythrocyte lysis ( BD , 349202 ) in ammonium chloride ( NH Cl , 8.26 g in ddH O ) , potassium bicarbonate ( KHCO , 1 g ) , EDTA ( 0.037 g ) buffer ( pH 7.3 ) , 50,000 to 100,000 , CD45 cells were analysed .