sentence: 200,000 cells per well of a 6-well plate were reverse transfected with scrambled ( R0K0 ) , SNX17 ( R6K4 ) , and SNX27 ( R10K8 ) siRNA oligos ( ON-TARGETplus SMARTpool ; Thermo Fisher Scientific ) with reagent ( HiPerFect ; QIAGEN ) according to the manufacturer ’ s reverse transfection protocol .