sentence: Plasmids YEpSBH1-FL ( DM ) L ( full length SBH1 with mutations P54S V57G ) , YEpSBH1-TM ( DM ) L ( tm-domain encoding SBH1 with mutations P54S V57G ) , YEpSBH1-TM ( SM1 ) L ( tm-domain encoding SBH1 with mutation P54S ) and YEpSBH1-TM ( SM2 ) L ( tm-domain encoding SBH1 with mutation V57G ) were transformed into H3543 strain containing plasmids encoding either Suc2 , Sec2 , Dap2 ( from N. Johnsson , University of Muenster , Germany , and J. Brown , Newcastle University , United Kingdom ) , or the empty vector pRS314 [ 1,36 ] .