sentence: By performing the Thioflavin T ( ThT ) kinetic assay and negative-staining transmission electron microscopy ( NS-TEM ) , we showed that G51D α - syn spontaneously forms long and unbranched amyloid fibrils with a lag time of ~ 16 h in 50 mM phosphate buffer , pH 7.0 , 50 mM NaCl ( referred to as PB buffer ) ( Fig. 1a ) .