sentence: Constructs were designed using an 18-bp sequence including the Shine-Dalgarno sequence from pBAD ( 35 ) followed by an ATG start codon fused to the DNA sequence occurring directly after that encoding for the characteristic Rhs PXXXXDPXGL motif or the sequence of the predicted immunity gene followed by that encoding the V5his tag ( from pET-DEST42 ( Invitrogen ) ) .