sentence: The term sequential model refers to surface infection , where one of the organisms plays a role as a first colonizer ( C. albicans or P. gingivalis , respectively ) and was grown overnight at optimal conditions after which the second coloniser was settled.In sequential model type I , the P. gingivalis biofilm was first formed by settling the bacterial cells on the microplate wells ( 1 × 10 cells / well ) in Schaedler medium followed by an overnight incubation at 37 ° C under anaerobic conditions maintained using a GENbox jar with a Genbox anaer generator ( bioM é rieux S.A ., Marcy l ’ Etoile , France ) .