sentence: This is consistent with the presence in this fraction of pre-40S particles from both the nucleoplasm ( referred to hereafter as pre40S-Nuc ) and the cytoplasm ( designated from now on as pre40S-Cyt ) that must be undergoing the sequential release of RRP12 and late RBFs ( ENP1 , PNO1 , TSR1 , LTV1 , RIO2 , and NOB1 ) along the maturation process .