sentence: Lariatin A and variants were separated from other components in the culture broth under the following conditions ; column , PEGASIL ODS ( 2.0 × 50 mm , Senshu Pak ) ; solvent , the 20-min linear gradient from 10 % to 100 % acetonitrile containing 0.05 % trifluoroacetic acid ( TFA ) ; column temperature , 40 ° C ; flow rate , 0.2 ml / min ; detection , UV 210 nm .