sentence: The coding region of the wild-type cDNA of MOT48 was amplified by PCR with the primers MOT48-NF2 ( 5 ′ - GGATCCCATATG­CTGATAACAGA­TAACATGCTAG­GGAAACAAGAA-3 ′ ) and MOT48-NR2 ( 5 ′ - GAATTC­CGGCTCCAGCT­CCAGCAGCGCC­TTGCTCGCAAA­CGACAG-3 ′ ) , which contained the recognition sites for BamHI – NdeI and EcoRI , respectively ( underlined ) .