sentence: In brief , gRNA encoding oligonucleotide cassettes ( gRNA1 [ 5 ′ - GCTGAACGCAGAGCGACTCG-3 ′ ] and gRNA2 [ 5 ′ - TCGCCACCTGGATCCGGTCG-3 ′ ] for ACBD3 ; gRNA1 [ 5 ′ - GTGTGGGGTACACGGACCACG-3 ′ ] and gRNA2 [ 5 ′ - GAGACTCGGGCAGGGAGCTTA-3 ′ ] for PI4KB ) were cloned into the SapI restriction sites of the pCRISPR-hCas9-2xgRNA-Puro plasmid .