sentence: Sequences of the primers were as follows : NKAP forward , 5 ′ - CGGCAGAAGAGATTAAGTGAG-3 ′ and reverse , 5 ′ - CGTTCATACCCCCAGAGGTTTAG-3 ′ , and β - actin forward , 5 ′ - CCCGAGCCGTGTTTCCT-3 ′ and reverse , 5 ′ - GTCCCAGTTGGTGACGATGC-3 ′ .