sentence: While we do not know the stoichiometry of S185 phosphorylation , this regulatory mechanism could function as an on / off switch at shmoo tips rapidly activated during stress responses rather than a global tunable rheostat typically observed for multisite phosphorylation.Although we were unable to confirm that S185 phosphorylation depends on Pkc1 or mechanical stress in vivo , several lines of evidence suggest that the RING-H2 domain of Ste5 is directly phosphorylated by Pkc1 .