sentence: 100 μ M α - syn WT , 100 μ M G51D monomer or mixed monomer ( 50 μ M WT + 50 μ M G51D ) was incubated with 10 μ M ThT in the reaction mixture in PB buffer ( 50 mM phosphate buffer , pH 7.0 , 50 mM NaCl , 0.05 % NaN ) or Tris Buffer ( 50 mM Tris , pH 7.5 , 150 mM KCl , 0.05 % NaN ) .