sentence: Rabbit polyclonal antibodies included anti-PI4KB ( Millipore ) , anti-myc ( Thermo Fisher Scientific ) , anti-TGN46 ( Novus Biologicals ) , anticalreticulin ( Sigma ) , anti-FLAG ( Sigma ) , and anti-EGFP ( a gift from J. Fransen , NCMLS , Nijmegen , The Netherlands ) .