sentence: Both methods revealed significant differential expression of only few contigs at the False Discovery Rate ( FDR ) of < 0.05 ( Fig. 4a , Additional file 2 : Figure S2 ) : DE isotigs identified using transcript abundances estimated by RSEM were 45 ( for a total of 82 contigs ) whereas Corset transcript quantification led to the identification of 59 clusters ( for a total of 85 contigs ) ( Additional file 3 : Table S2 , Additional file 4 : Table S3 ) .