sentence: The serum was separated by centrifugation within twelve hours of collection and stored at − 20 ° C. Dacron swabs ( Fischer Scientific , Loughbourgh , UK ) were used to swab the facial area and antebrachium gland.Squirrel serum was analyzed for SQPV antibodies using an enzyme-linked immunosorbent assay ( ELISA ) ( Sainsbury et al. 2000 ) .