sentence: 72 h post transduction , MEFs and G401 cells were harvested and lysed with buffer A ( 20 mM HEPES , pH 7.4 , 10 mM KCl , 1.5 mM MgCl , 0.34 M sucrose , 10 % glycerol , 1 mM dithiothritol and protease inhibitor cocktail ) containing 0.2 % Triton X-100 .