sentence: MATa cells growing in mid log phase were treated with 2.7 µ M α factor for 100 min and loaded in the microfluidic chip , which was precoated with 1 mg / ml Con A. Where appropriate , cells were pretreated for 15 min with the solvent DMSO or , as indicated , with cercosporamide ( 7.5 µ M ; Merck ) or NaPP1 ( 5 µ M ; Tocris Bioscience ) .