sentence: GV3101 cells containing PYL192 ( TRV1 ) and TRV : : CaHSL1-1 , TRV : : CaHSL1-2 or TRV : :00 ( as a negative control ) were resuspended in the induction medium at 1:1 ratio ( OD = 0.6 ) used in the VIGS following the method in our previous study ( Dang et al ., 2013 ) , and were co-infiltrated into cotyledons of 2-week-old pepper plants .