sentence: Laser injury assay : cells were injured at 37 ° C in cell imaging media ( CIM : Hank ’ s balanced salt solution +10 mM Hepes +2 mM Ca , pH 7.4 ) by pulsed UV-laser ( Rapp OptoElectronic or visible light ( 532 nm ) - laser ( AblateTM , 3i Intelligent Imaging Innovations , Inc. Denver , CO , USA ) by irradiating a small region ( 1 – 2 µ m ) for < 10 – 100 ms.