sentence: Altogether , these data suggest that TFIP11 knockdown might cause structural rearrangement in the U4 / U6.U5 tri-snRNP and alter the formation and / or stability of spliceosomal intermediates , impacting the dynamics and assembly of the spliceosome machinery.As incomplete or immature tri-snRNP intermediates can accumulate in CBs , we checked for co-localization between coilin and the U4 / U6 di-snRNP-specific protein Prp4k or the U5 snRNP-specific protein EFTUD2 in TFIP11-depleted cells .