sentence: Slides of paraffin-embedded squamous cell carcinoma tumor specimens , dysplastic and normal epithelial tissues were deparaffinized , rehydrated and sections were treated with antigen retrieval buffer ( pH 6 , S2367 , DAKO , Carpinteria , CA ) in a steamer for 20 min at 96 ° C. Anti-WHSC1L1 antibody ( 11345-1-AP , Proteintech , Rosemont , IL , dilution 1:400 ) was applied on tissue sections for one hour incubation at room temperature .