sentence: In addition , we further investigated the expansion of a specific candidate gene within Jaltomata , SEUSS , by examining gene trees , primary mapped Illumina reads ( i.e ., using only the best alignment of multimapped reads ) , PacBio reads spanning more than one gene copy , and expression patterns supported by RNA-seq from 14 Jaltomata species examined in our previous study ( Wu et al. 2018 ) ( see supplementary text , Supplementary Material online ) .