sentence: Paraformaldehyde or formalin-fixed tissue sections from temporal cortex ( middle temporal gyrus ) were used for localization of progranulin ( PGRN ) - positive cells identified with antibody AF2420 ( R & D Systems , Minneapolis , MN , U.S.A. ) , and for colocalization with A β peptide and phosphorylated tau , and markers of microglia ( IBA-1 , CD45 ) , astrocytes ( GFAP ) , endothelial cells ( CD31 ) , lysosomal proteins ( LAMP-1 , CD68 , prosaposin , cathepsin D ) and others ( sortilin , beta-secretase-1 ( BACE1 ) , TMEM106B , neurofilaments , synaptophysin ) according to our previously published procedures [ 51 , 52 ] .