sentence: After washing , primary phospho-specific antibodies were added at 40 µ l / well in TBS , 0.05 % Tween20 , 0.1 % BSA , for 2 h at room temperature ( H3S28ph 0.2 µ g / ml , H3T3ph 0.1 µ g / ml , P-Tyr-100 0.15 µ g / ml , INCENP S446ph 1:5000 , BCL9L S915ph 1:5000 ) .