sentence: Due to the localisation of both ANKS3 and ANKS6 to the primary cilium in the mouse kidney [ 7 ] and in Zebrafish [ 17 ] , and experimental evidence ofANKS3 interaction with nephronophthisis proteins [ 4 , 17 ] , we tested the effects of in vivo Anks3 knock down on renal expression of genes encoding proteins present in the basal body ( Cep290 , Nphp5 ) and the transition zone ( Nphp1 , Nphp2 , Nphp4 ) of the primary cilium , and in the ciliary axoneme ( Nek8 , Gli2 ) [ 18 , 19 ] ( S3 Fig ) .