sentence: Genomic DNA of transformant clones that were chloramphenicol sensitive and streptomycin resistant was used as a template for PCR to screen for replacement of the target gene with rpsL-cat with primers JG402 / JG407 fzor HP0381 , JG378 / JG383 for HP1369 , JG384 / JG389 for HP1471 , JG390 / JG395 for HP1522 , and JG396 / JG401 SOE-PCR was used to amplify the target gene from OND79 genomic DNA using primers in which the homopolymeric tract sequence had been altered .