sentence: The previously constructed anti-IncF cassette from pCURE2 , designed to displace IncF plasmids , including loci that inhibit replication ( repFIA , incC ; repFIB ; repFIC , copAB ; repFIC / repFIIA , copAB ) and that neutralise addiction systems ( flmB , sok ; letA , ccdA ; pemI , srnC , sok ) [ 12 ] , was inserted into RK2 by recombination to replace the aph ( Km ) gene ( Fig 2 ) as described in Methods , creating pCURE-F-RK2 ( Fig 3 ; nomenclature indicates first that it carries a curing cassette , second the plasmid group it targets , and third the plasmid it is based on ) .