sentence: PCR primers for PAF synthases , PAF receptor and glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ) cDNA were designed as follows : LPCAT1 ( accession number NM_001100735 ) primers , sense 5 ' - CCATCCGGCTCCTGTTTGCT-3 ' and antisense 5 ' - CGCCCGTCGCTTGATCTCTT-3 ' ; LysoPAFAT / LPCAT2 ( accession number XM_001064713 ) primers , sense 5 ' - CCTCGCCAGGCGTCCTTCTT-3 ' and antisense 5 ' - GAACATGGCACGACCCAGGA-3 ' ; PAF receptor ( accession number U04740 ) primers , sense 5 ' - GGCTCCTTCCGTGTGGATTC-3 ' and antisense 5 ' - GCAGCGGGTGATGTTACCTG-3 ' ; GAPDH ( accession number M17701 ) primers , sense 5 ' - CCAGGGCTGCCTTCTCTTGT -3 ' and antisense 5 ' - CCAGCCTTCTCCATGGTGGT -3 ' .