sentence: Therefore , C3bot was cloned in place of the original TcC3hvr , that is , C-terminal to the auto-proteolytic cleavage site of truncated BC3 ( functional cocoon-forming BC fusion protein of P. luminescens TcB2 and TcC3 [ Meusch et al , 2014 ] ) and the chimeric complex ( TcB-TcC3 - C3bot [ BC3-C3bot ] ) was expressed in Escherichia coli ( Fig 1A ) .