sentence: Briefly , the flanking regions of target genes were amplified by SOE-PCR [ 18 ] from OND79 genomic DNA with Phusion DNA polymerase ( NEB ) with primer pairs JG379 / JG380 and JG381 / JG382 for HP1369 , JG385 / 386 and JG387 / JG388 for HP1471 , JG391 / JG392 and JG393 / JG394 for HP1522 , JG397 / JG398 and JG399 / JG400 for HP0464 , and JG403 / 404 and JG405 / 406 for HP0381 .