sentence: To assess the relative increase in intracellular c-di-GMP with dccA induction during growth on 70:30 sporulation agar , we employed the previously described reporter controlled by the regulatory region of the pilA1 locus : the pilA1 promoter ( P ) and the 5 ′ untranslated region ( UTR ) containing a c-di-GMP riboswitch ( 41 ) .