sentence: Lentiviral particles were generated in the 293FT packaging cell line ( complete medium : high glucose DMEM , 10 % FBS , 0.1 mM NEAA , 1 mM MEM Sodium Pyruvate , 6 mM L-Glutamine , 1 % Pen / Strep and 0.5 mg / ml Geneticin ) by transient transfection of transfer vector , either pLenti6.3 / v5-DEST-SMDF ( SMDF ) or pLenti6.3 / V5-DEST-Hrg β -1 ( Hrg β -1 ) , 2nd generation packaging plasmid-psPAX2 and envelope vector-pMD2 ( kind gifts from Didier Trono , Addgene plasmids # 12259 and # 12260 , respectively ) with the ratio ( 7:2:1 ) using calcium-phosphate precipitation method [ 63 ] .