sentence: Analysis of ProMYB64 : MYB64-GFP and ProMYB119 : MYB119-GFP in cki1 mutants was done by emasculating stage 12c flowers and examining the ovules 12 hours later.For CSLM analysis of gametophyte development , pistils were sliced open along the replum using a needle and immersed in fixative containing 1 × PBS and 4 % glutaraldehyde .