sentence: Plasmids pT3-Hcp1 , pT3-ClpV1 , and pT3-ClpV2 were generated by cloning of a PCR product containing the corresponding hcp1 , clpV1 , and clpV2 regions of E. cloacae , respectively , into the pMPM-T3 plasmid ( see primers in Table 2 ) .