sentence: Fibroblasts were resuspended in treatment media at 80,000 cells / mL and then seeded into 384-well imaging plates ( Greiner microclear , Greiner Bio-One GmbH , Kremsmunster , Austria ) at 25 µ L / well , with a further 25 µ L of media with or without 10 ng / mL rhTGF β ( R & D Systems , Minneapolis , MN , 7754-BH / CF ; final concentration , 5 ng / mL ) .