sentence: Several grams of different tissues from each plant were frozen in liquid nitrogen and stored at − 80 ° C.JrFAD3-1 gene expression analysis was conducted with ‘ Qingxiang ’ samples from 10 different developmental stages ( 70 DAF , 80 DAF , 90 DAF , 95 DAF , 100 DAF , 105 DAF , 110 DAF , 120 DAF , 130 DAF and 140 DAF ) , and 8 different tissues : pistils , catkins , young branches ( bark ) , old branches ( bark ) , young leaves , mature leaves , young embryos and mature embryos .