sentence: A commercial polyclonal rabbit antibody from Novus Biologicals ( Littleton , CO ) was used for GLUT9 , and rabbit polyclonals from Cell Signaling Technology ( Danvers , MA ) were used for p ‐ thr 308 Akt ( 9275 ) , Akt ( 4685 ) , transforming growth factor beta 1 ( TGF β , 3711 ) , and the mammalian target of rapamycin ( mTOR , 7C10 ) .