sentence: The genes encoding the two Nfo homologs , PF0258 and PF1383 , were amplified by PCR directly from P. furiosus genomic DNA , using the oligonucleotides 5 ′ - dGCGCCATATGTTCAAAATTGATAGGCTAAG-3 ′ and 5 ′ - dGCGCGGATCCTTAAATTTTTAGCTCCTCCC-3 ′ as the forward and reverse primers for PF0258 and 5 ′ - dGCGCCATATGAAAGTTGGAGTTAGCATATA-3 ′ and 5 ′ - dGCGCGGATCCTCACCTCATCAATCTCTGAA-3 ′ as the forward and reverse primers for PF1383 .