sentence: These results indicate that a large majority of the protein-coding transcripts that overaccumulate in xrn4 are in the polyA - fraction.To validate several of these transcripts identified by RNA-seq as overaccumulating in xrn4 , northern blots of total RNA were compared alongside fractionated ( polyA + and polyA - ) RNA in biological replicates different from those used for the RNA-seq ( Supplementary Figure S1 ) .