sentence: In short , 40 µ g of mVDAC1 ( containing one exposed and one buried cysteine ) or 20 µ g of hVDAC3 ( containing five exposed and one buried cysteine ) were mixed with 16 µ g of CPM in a final volume of 1 ml of buffer containing 40 mM HEPES , pH 7.0 , 50 mM NaCl , and 0.1 % LDAO .