sentence: Interestingly , reducing the size of the DNA targets ( 45 – 102 bp ) resulted in a decrease in the protein / DNA ratio ( only 1 ∶ 50 ) required to obtain a shift , ( Fig. 2B ) , showing that we indeed were able to increase the affinity as we narrowed down the targets to match the DRs.The transcription initiation sites were determined for lipF , pks2 and msl3 in previous studies [ 31 ] , [ 32 ] .