The route parallels the Appalachian Mountains for much of its route through Tennessee and Virginia , serving such cities as the twin cities of Bristol , Tennessee and Bristol , Virginia , Roanoke , Blacksburg , Lexington , Staunton , Harrisonburg , and Winchester .
The route follows the Appalachian Mountains for much of its route through Tennessee and Virginia , serving such cities as the twin cities of Bristol , Tennessee and Bristol , Virginia , Roanoke , Blacksburg , Lexington , Staunton , Harrisonburg , and Winchester .