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[DESCRIPTION]
<p>MegaLong™ isolates high molecular weight (&gt;100kb) genomic DNA from a variety of samples, including animal tissues, cultured cells, whole blood, bacterial and yeast.</p>
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<p>The protocol described here is for yeast samples. Please <a href="https://www.protocols.io/view/MegaLong-Protocols-Collection-for-Isolation-of-100-e7kbhkw" target="_blank">refer to the appropriate protocol</a>, depending on your application.</p>

[GUIDELINES]
<p><strong>INTRODUCTION </strong></p>
<p>MegaLong™ isolates high molecular weight (&gt;100kb) genomic DNA from a variety of samples, including animal tissues, cultured cells, whole blood, bacterial and yeast.  MegaLong™ uses Genomic Tube-O-DIALYZER™, a unique micro dialysis device with a 0.45µm membrane, which minimizes sample manipulation, one of the main reasons for DNA breakage.  MegaLong™ isolates nuclei under mild extraction conditions and releases genomic DNA by digestion of nuclear proteins with a highly active LongLife™ Proteinase K. The digestion is performed in the Tube-O-DIALYZER™ and after digestion the Tube-ODIALYZER™ is inverted to dialyze away digested protein and other impurities leaving behind highly pure and fully hydrated genomic DNA.  </p>
<p><br />The fragile, high molecular weight genomic DNA can be stored in the Tube-O-DIALYZER™ to further minimize mechanical manipulation of the DNA. The DNA is suitable for Southern blot analysis, recovery of Lambda shuttle vectors from transgenic animals, PCR, analysis by pulsed-field electrophoresis or any application where genomic DNA is required.</p>
<p><br /><strong>APPLICATIONS</strong></p>
<p>MegaLong™ kit can be used for the isolation of genomic DNA from animal tissues, cultured cells, whole blood, bacterial and yeast.  For samples unsuitable for the isolation of high molecular weight DNA with MegaLong™, G-Biosciences recommends using the OmniPrep™ Genomic DNA isolation kit (Cat. # 786-136). </p>
<p><br />The kit is supplied as a Micro or Large packs to process either 25 or 50 1-25mg samples.   </p>
<p><br /><strong> ITEM(S) SUPPLIED</strong></p>
<table style="width: 523px; height: 178px;">
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<td style="width: 172px;"><strong> Description</strong></td>
<td style="width: 163px;"><strong>Cat. # 786-146 </strong></td>
<td style="width: 166px;"><strong>Cat. # 786-147</strong></td>
</tr>
<tr>
<td style="width: 172px;">Nuclei Isolation Buffe</td>
<td style="width: 163px;">2 x 30ml</td>
<td style="width: 166px;">4 x 30ml</td>
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<tr>
<td style="width: 172px;">Suspension Buffer</td>
<td style="width: 163px;">1 x 10ml</td>
<td style="width: 166px;">2 x 10ml</td>
</tr>
<tr>
<td style="width: 172px;">Digestion Buffer</td>
<td style="width: 163px;">1 x 2ml</td>
<td style="width: 166px;">2 x 2ml</td>
</tr>
<tr>
<td style="width: 172px;">LongLife™ Proteinase K [5mg/ml]</td>
<td style="width: 163px;">2 x 0.5ml</td>
<td style="width: 166px;">4 x 0.5ml</td>
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<tr>
<td style="width: 172px;">Genomic Tube-O-Dialyzer™  </td>
<td style="width: 163px;">   25  </td>
<td style="width: 166px;">   50</td>
</tr>
<tr>
<td style="width: 172px;">Floats (Medi)    </td>
<td style="width: 163px;">   6</td>
<td style="width: 166px;">   6</td>
</tr>
<tr>
<td style="width: 172px;">Caps (Medi)</td>
<td style="width: 163px;">   25    </td>
<td style="width: 166px;">   50</td>
</tr>
<tr>
<td style="width: 172px;">Forceps</td>
<td style="width: 163px;">   1</td>
<td style="width: 166px;">   1</td>
</tr>
</tbody>
</table>
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<p> <br /> <br /><strong>STORAGE CONDITION</strong></p>
<p>The kit is shipped at ambient temperature. Upon arrival, store LongLife™ Proteinase K at -20°C and remaining components at 4°C.  LongLife™ Proteinase K solution is stable for 1 year, if stored properly.</p>
<p><br /><strong>ADDITIONAL MATERIALS REQUIRED</strong> </p>
<p>• Microfuge tubes &amp; pestles (Cat. # 786-138P) </p>
<p>• TE buffer</p>
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