eamag/protocols-with-steps huggingface dataset:
TITLE: HotSHOT genomic DNA extraction
AUTHORS: FishFloorUCL

[DESCRIPTION]
How to extract genomic DNA from larvae or finclips using the HotSHOT method.

[STEPS]
SECTION: Materials
1. Prepare the BASE solution (50X):
14.03 g KOH crystals (1.25M final concentration)
4 mL of 0.5M EDTA (10 mM final concentration)
ddH2O to 200 mL total volume
SECTION: Materials
2. Prepare the NEUTRALISATION solution (50X)
63.04 g Tris-HCL (2M final concentration); also called Trizma HCl
ddH2O to 200 mL total volume
SECTION: Procedure
3. Prepare fresh 1X BASE and NEUTRALISATION solution in nuclease-free H2O.
SECTION: Procedure
4. If larvae: add larvae one per well with Pasteur pipette. Take up all the liquid using a P200 pipette.
If finclip: add finclip directly to well (this can be done while finclipping).
SECTION: Procedure
5. Add 50 µL of 1x BASE Solution into each well of the plate.
SECTION: Procedure
6. Seal the plate and place on PCR block:
95 °C 30 min 
SECTION: Procedure
7. Cool at
Room temperature 

SECTION: Procedure
8. Add 50 µL of 1x NEUTRALISATION solution into each well of the plate.
SECTION: Procedure
9. Note, the extracted DNA concentration is often too high for downstream applications like PCR or KASP.
SECTION: Storage
10. Store at
4 °C if you will use the DNA in the next few weeks. Beware, the samples will slowly evaporate from sealed plate
or
-20 °C for long-term storage. This will also prevent the samples from evaporating.
TITLE: Weight Crasher Keto Gummies Reviews - Fake Or Trusted?
AUTHORS: weightcrasherreviews  

[DESCRIPTION]
Weight Crasher Keto Gummies Reviews - Fake Or Trusted?


[STEPS]
1. Weight Crasher Keto Gummies Reviews - Fake Or Trusted?
Weight Crasher Keto Gummies Review Rotundity is a wide problem that most individualities are dealing with. People who have poor eating habits and don't engage in visionary conditioning gain weight because their bodies warrant fat- burning mechanisms. There are traditional weight loss options on the request. The maturity of people consider traditional options but don't take action. The weight loss trouble is hampered by tight deadlines and a lack of ambition. It's critical to make similar opinions that will help you in barring any redundant fat from your body. The same may be said about delicate eating habits and exercise habits that make a person ineffective in their weight loss sweats. 
Weight Crasher Keto Gummies help to strengthen the body and boost metabolism. It makes it simple for the person to stick to their diet plan. It prevents the body from storing fat and rather utilizes it to induce energy. This approach enhances well- being and makes a person really and clairvoyantly calm. It's well- known for being a lately dispatched item. This helps the customer achieve the topmost results by reducing the inflexibility of all ails in the constitution. 
What Are Weight Crasher Keto Gummies? 
The system is forced to eat fats to separate the needed energy. Sugars pullulate in the food we eat, making it a simple source of energy. As a result, the body absorbs carbohydrates rather of fats, accumulating fats and working to lose weight. When the system runs out of carbs, it turns to lipids and the ketones produced by the liver to keep ketosis going. So ketosis is the process of carrying energy by ingesting fat factors rather of carbohydrates. 
Weight Crasher Keto Gummies is a ketogenic natural result that supports weight loss pretensions by perfecting metabolism rate and adding ketones and fat burn. It’s composed of all-natural organic fat- burning constituents that are combined in the applicable quantities and packaged in easy-to- take goo. Crasher Keto Gummies contain beta-hydroxybutyrate, hydrolyzed collagen, zinc, vitamin D, and fish canvas greasepaint, among other constituents. This important list of constituents effectively causes ketogenic, a metabolic condition in which accumulated fats rather of carbs are consumed for energy. Each element is strictly anatomized in laboratories by good medical experts and manufactured in a US installation that meets established norms. 
Official Website:- https://freaknewswire.com/index.php/2022/05/13/weight-crasher-keto-gummies-reviews-is-it-really-works/
TITLE: No Title
AUTHORS: 

[BEFORE_START]
<p>Prepare 5 μM primer solutions:</p>
<p> </p>
<div title="Page 1">
<p>ITS2 primers used in the 1st PCR:</p>
<p>ITS2-4R GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNTCCTCCGCTTATTGATATGC</p>
<p>ITS2-S2F ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNATGCGATACTTGGTGTGAAT</p>
<p> </p>
<p>Indexing primers used in the 2nd PCR (xxxxxxxx - index):</p>
</div>
<div title="Page 1">AATGATACGGCGACCACCGAGATCTACACxxxxxxxxACACTCTTTCCCTACACGACGC</div>
<div title="Page 1">
<div>
<p>CAAGCAGAAGACGGCATACGAGATxxxxxxxxGTGACTGGAGTTCAGACGTGTGC</p>
</div>
</div>

[GUIDELINES]
<p>Perform reactions in small batches until you are confident that there is no cross-contamination among the samples. Including isolation blanks and PCR blanks is crucial for the quality control.</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: GUARDS Statistical Analysis Plan 
AUTHORS: Professor  Catherine Williamson, Dr Jose Blanco Carnero, Dr Catalina de Paco Matallana, Dr  Alice Mitchell, Dr  Caroline Ovadia, Professor David Wright

[DESCRIPTION]
GUARDS is a double-blind randomised placebo-controlled single site trial. The participating centre in Spain will be Clinic University Hospital “Virgen de la Arrixaca” in Murcia. Women with an abnormal oral glucose tolerance test (OGTT) screen will be offered participation. After written informed consent, eligible women will be randomly assigned to UDCA or placebo at 24+0-30+6 weeks’ gestation until birth. All participants will be offered standard antenatal care, aiming for the GEDE guideline targets of blood glucose concentration between 3.9-7.8mmol/L (70 - 140 mg/dL). We will assess clinical efficacy, mechanisms of action and acceptability of UCDA in study participants. Clinical efficacy will be measured by maternal-infant glucose control using conventional capillary and novel continuous glucose measures and standard clinical measures for serum lipids (total, HDL- and LDL-cholesterol, triglycerides and total free fatty acids). Acceptability will be measured by patient compliance (pill counts) and confirmed by serum measurements of individual bile acids (including UDCA). 

Serum samples will be taken for UPLC-MS/MS measurement of bile acids (including UDCA), so it will be clear if women have taken (and absorbed) the drug. Obstetric and neonatal outcomes will be measured. This Statistical Analysis Plan sets out how we will analyse the trial.

[STEPS]
TITLE: Fear Conditioning
AUTHORS: Haley   Geertsma

[DESCRIPTION]
This protocol is used to test mice in the Fear Conditioning behavioural test.

[STEPS]
1. On day 1, confirm that EthoVision is set up properly for a 6-minute test (with 3 tone-shock pairings of a 30-second tone co-terminated with a 2-second foot shock).
2. Place mice into the Fear Conditioning apparatus and start the testing.
3. Once testing is complete, return mice to their home cage overnight.
4. On day 2, confirm that EthoVision is set up properly for a 6-minute test (no tone or foot shock).
5. Place mice into the Fear Conditioning apparatus and start the testing.
6. Once testing is complete, return mice to their home cage overnight.
7. On day 3, confirm that EthoVision is set up properly for a 6-minute test (with 3 minutes of no tone/shock, then 3 minutes repeating the 30-second tone without a foot shock). Confirm that the room has a novel scent, noise, and Fear Conditioning apparatus).
8. Place mice into the Fear Conditioning apparatus and start the testing.
9. Once testing is complete, return mice to their home cage.
TITLE: Protein Transfer using Bio-rad TransBlot Turbo
AUTHORS: Steven Burgess

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A protocol for transfer of proteins from acrylamide gels onto nitrocellulose membrane for immunoblot analysis. This protocol is based on the assumption that TGX pre-cast gels have been used, the procedure is very similar for self-made gels, just that the running time is longer. Using the transfer packs is economically similar to wet-transfer using MeOH.  </div><div class = "text-block"><span style = "font-weight:bold;">Note</span><span>:  Clean the apparatus immediately after use with dH</span><span style = "vertical-align:sub;">2</span><span>O and dry. This prevents the build-up of salts and rust which impair performance.  </span></div><div class = "text-block"><span style = "font-weight:bold;">Literature</span><span>: </span></div><div class = "text-block"><span>Full manual: </span><a href="https://www.bio-rad.com/webroot/web/pdf/lsr/literature/10020688.pdf" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;">https://www.bio-rad.com/webroot/web/pdf/lsr/literature/10020688.pdf</span></a><span style = ":;"> </span></div><div class = "text-block"><span>Quick-start: </span><a href="https://www.bio-rad.com/webroot/web/pdf/lsr/literature/10016505D.pdf" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;">https://www.bio-rad.com/webroot/web/pdf/lsr/literature/10016505D.pdf</span></a><span style = ":;"> </span></div><div class = "text-block"><span>Transfer pack: </span><a href="https://www.bio-rad.com/webroot/web/pdf/lsr/literature/10019593D.pdf" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;">https://www.bio-rad.com/webroot/web/pdf/lsr/literature/10019593D.pdf</span></a></div></div>

[STEPS]
?. Place the membrane and bottom stack in the middle of the cassette base
?. Place the pre-cast gel (following SDS-PAGE electrophoresis) in the middle of the membrane and roll to remove air-bubbles
?. Place the top stack on top of the gel, gently roll
?. Close the cassette lid, taking care not to disturb the gel
?. Place the cassette in the Trans-Blot Turbo and follow the instructions on the machine (Fast protocol TGX gel).
?. Either dry membrane and store at  for later use or proceed immediately to fluorescent western protocol
4 °C
TITLE: Parse Evercode WT Mini v2.0.1 Protocol -- University of Minnesota TMCs
AUTHORS: Parse Biosciences, Laura J Niedernhofer, David A Bernlohr

[DESCRIPTION]
https://www.parsebiosciences.com/products/evercode-whole-transcriptome

Protocol for Parse Biosciences Evercode, MINI WT -- for trials/pilots, capable of tagging 10k cells total in up to 12 samples. 

Version 2.0.1 

https://www.parsebiosciences.com/

[STEPS]
1.   

2.
TITLE: Preparation of electrocompetent cells
AUTHORS: Shuning    Guo

[DESCRIPTION]
This protocol is used to prepare electrocompetent cells with high transformation efficiency.

[BEFORE_START]
All the medium and containers used in the protocol should be sterilized by high temperature autoclave.
All the steps that expose cells to air should be down in the super-clean worktable.

[STEPS]
1. Line the E. coli strain on a solid LB medium without resistance and culture at 37℃ for 12h.
 

2. Pick monoclonal cells from the medium and culture them in 5 ml LB medium at 37℃ for 12h.  

 

3. Inoculate 100 ml of LB medium with 1% volume of E. coli culture from step 2.
 

4. Grow the cells at 37℃ shaking at 200 rpm to an OD600 of 0.4 (about 1h 20min).
 

5. Transfer the culture on the ice immediately and chill cells on ice for 30 min and chill the centrifuge at 4 ℃ at the same time.
6. 
 

7. Transfer the cells to two cold 50ml centrifuge bottles (45ml every bottle) and spin at 1000 x g for 15 min at 4℃.
8. Carefully pour off and discard the supernatant.
9. Gently resuspend the pellet in ice-cold ddH2O (43ml every bottle). Centrifuge at 1000 x g for 20 min at 4℃; carefully pour off and discard the supernatant.
10. Gently resuspend the pellet in ice-cold 10% glycerol (21ml every bottle). Centrifuge at 1000 x g for 20 min at 4℃; carefully pour off and discard the supernatant.
11. Gently resuspend the pellet in ice-cold 10% glycerol (858μl every bottle). Mix well and transfer the mixture in two bottles into on bottle. Centrifuge at 1000 x g for 20 min at 4℃; carefully pour off and discard the supernatant.
12. Gently resuspend the pellet in 172 μl ice-cold GYT medium. 
13. Transfer the suspension into ice-cold 1.5ml microfuge tubes (25μl every tube).
14. Freeze the suspension in -80℃ ice cuber for about 2h.
TITLE: 城市微生物菌相調查計畫——採集土壤教學
AUTHORS: Hsin-Mao  Wu

[DESCRIPTION]
Soil sample, citizen scientist, 土壤樣本採集，公民科學家

[STEPS]
SECTION: 如何加入專案
1. 開啟Line app，將頁面切換成「聊天」，並按下頂部的掃描圖示(紅框處)
  

SECTION: 如何加入專案
2. 掃描以下QR code或點選此連結微生物公民科學
  

SECTION: 如何加入專案
3. 加入成功後會顯示以下畫面
  

SECTION: 如何加入專案
4. 接著等待採集工具包寄送即可開始採集！
SECTION: 採土
5. 移除表土，取距離土表5~7公分之處的土，裝入塑膠夾鏈袋中
[示意圖片待補]
SECTION: 採土
6. 將塑膠袋中的土均勻混合，倒入採集罐之中
 

SECTION: 採土
7. 在採集紀錄紙上勾選採樣地點、採樣種類，以及填寫日期、城市、鄉鎮、地點、採集者等資訊
SECTION: 採土
8. 最後拍兩張照片
 

   
   

 

SECTION: 採土
9. 打開iNaturalist app，上傳剛剛拍攝的兩張照片
[上傳之螢幕截圖與步驟待補]
SECTION: 採土
10. 上傳完成後，在詮釋資料中找到ID(如下圖紅框處)  
 


SECTION: 採土
11. 在採集紀錄紙上填入ID，並將紀錄紙放入採集罐中
[示意圖片待補]
SECTION: 採土
12. 將採集罐保存於室溫，並於10天內以郵信寄回高雄醫學大學生物系
TITLE: No Title
AUTHORS: 

[STEPS]
SECTION: Day 1 Materials to Prepare
?. 
SECTION: Day 1 Procedure
?. 
SECTION: Day 1 Procedure
?. 
SECTION: Day 1 Procedure
?. 
SECTION: Day 1 Procedure
?. 
SECTION: Day 1 Procedure
?. 
SECTION: Day 1 Procedure
?. 
SECTION: Day 1 Procedure
?. 
SECTION: Day 1 Procedure
?. 
SECTION: Day 1 Procedure
?. 
SECTION: Day 2 Materials to Prepare
?. 
SECTION: Day 2 Procedure
?. 
SECTION: Day 2 Procedure
?. 
SECTION: Day 2 Procedure
?. 
SECTION: Day 2 Procedure
?. 
SECTION: Day 2 Procedure
?. 
SECTION: Day 2 Procedure
?. 
SECTION: Day 2 Procedure
?. 
SECTION: Day 2 Procedure
?. 
SECTION: Data Analysis
?. 
SECTION: Day 2 Procedure
?.
TITLE: ssDNA2.0: Dephosphorylation mix
AUTHORS: Sarah Nagel, Anna Schmidt, Matthias Meyer

[DESCRIPTION]
Protocol for the preparation of Dephosphorylation mix for automated single-stranded DNA library preparation using the ssDNA2.0 method (Gansauge et al. 2020). 

References
Gansauge, M.-T., Aximu-Petri, A., Nagel, S., & Meyer, M. (2020). Manual and automated preparation of single-stranded DNA libraries for the sequencing of DNA from ancient biological remains and other sources of highly degraded DNA. Nature Protocols, 15, 2279-2300.

[STEPS]
TITLE: Elevated Plus Maze
AUTHORS: Lauren Smith, Lani Tieu, Olivier George

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The elevated plus maze is used to measure anxiety-like behavior in rodents, and to assess the anxiolytic effects of pharmacological compounds. Time in the open arms of the maze reflects anti-anxiety-related behavior.</div></div>

[STEPS]
?. [Procedure]
Place rat in center of elevated plus maze and set the timer to 5 minutes.
?. [Procedure]
Use stopwatch to record the amount of time spent in open arms.In addition, tally the number of entries into each arm (open or closed).
At least 3/4 of the rat's body must enter the arm to count as time spent in that arm.
?. [Procedure]
Clean the maze with quatricide before testing the next rat.
Make sure to clean the maze in between each rat.
?. [Procedure]
Place the rat back in its home cage.
?. [Procedure]
After 5 minutes is up, calculate and write down the total amount of time spent in open arms and the total amount of time spent in closed arms.
TITLE: UroMOCA and StimPod Device Implantation
AUTHORS: Dennis Bourbeau, Brett Hanzlicek

[DESCRIPTION]
This protocol describes the implantation procedure of the wireless bladder device (UroMOCA) and of the wireless stimulation device (StimPods) into pigs.

[BEFORE_START]
Perform standard surgical prep.

[STEPS]
SECTION: UroMOCA implantation into bladder
1. Clean the incision site using Betadine solution and isolate the prep area with sterile towels.  Sterile instruments will be used.
SECTION: UroMOCA implantation into bladder
2. Place animal in supine position.
SECTION: UroMOCA implantation into bladder
3. Insert single lumen 8 Fr catheter.
SECTION: UroMOCA implantation into bladder
4. Administer analgesic at injection site.
SECTION: UroMOCA implantation into bladder
5. Expose the bladder by making a dorsal midline incision.
SECTION: UroMOCA implantation into bladder
6. Incise bladder dome.
SECTION: UroMOCA implantation into bladder
7. Place UroMOCA device into bladder.
SECTION: UroMOCA implantation into bladder
8. Close bladder incision and evaluate integrity.
SECTION: UroMOCA implantation into bladder
9. Place sterile drape on abdomen for UroMOCA transmission coil.
SECTION: UroMOCA implantation into bladder
10. Verify function of UroMOCA in vivo.
SECTION: UroMOCA implantation into bladder
11. Close abdominal incision.       
SECTION: StimPod Implantation
12. Place animal in prone position.
SECTION: StimPod Implantation
13. Administer analgesic at injection site.
SECTION: StimPod Implantation
14. Disinfect and drape lower back.
SECTION: StimPod Implantation
15. Make a dorsal midline sacral incision to expose the sacral spine from the second-to-last lumbar dorsal spinal process to the end of the sacrum.
SECTION: StimPod Implantation
16. Perform a laminectomy to remove the bone of the spine to expose the sacral roots from S1 to S2.
SECTION: StimPod Implantation
17. Implant 4 nerve cuff electrodes, including one each around the sacral root S1 left side, S1 right side, S2 left side and S2 right side.
SECTION: StimPod Implantation
18. Place EMG electrodes along the pelvic floor.
SECTION: StimPod Implantation
19. All electrodes will be attached to 2 wireless, implantable stimulation devices (StimPod, Micro-Leads, Boston, MA).
SECTION: StimPod Implantation
20. Verify function of StimPods in vivo (Power, Data transmission).
SECTION: StimPod Implantation
21. Confirm motor response (visual and EMG) to sacral root stimulation on each nerve cuff electrode.
SECTION: StimPod Implantation
22. Close back incision.
SECTION: StimPod Implantation
23. Monitor animal until recumbent.
TITLE: Make a worm picker
AUTHORS: Cristian Riccio

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Make a worm picker. Platinum is used because it cools down very quickly after sterilisation under a flame.</div></div>

[STEPS]
?. Unscrew the loop holder.
?. Cut 8 cm of platinum wire
?. Stick the platinum wire into the loop holder and screw the loop holder.
?. With your nails, flatten the end of the platinum wire. This is so as to make it into a bit of a spoon to collect the worms.
?. Your worm picker is ready! Sterilise under the flame before picking worms.
TITLE: Protein Concentration Determination using Qubit 4 Fluorometer
AUTHORS: Steven Burgess

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Procedure for quantification of protein concentration using a Qubit 4 Fluorometer. The procedure follows the manufacturer's instructions, this version is adapted for use with samples that have been extracted in protein extraction buffer as part of processing soybean leaf tissue. </div><div class = "text-block">Consult the manual for further details:  </div><div class = "text-block"><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/Qubit_Protein_Assay_UG.pdf" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;">https://assets.thermofisher.com/TFS-Assets/LSG/manuals/Qubit_Protein_Assay_UG.pdf</span></a></div></div>

[STEPS]
?. [Create Working Solution for Analysis]
Create a working solution of Qubit assay buffer by diluting the reagent 1:200 in the provided buffer.
The final volume in each tube must be 200 µL. Each standard tube requires 190 µL of Qubit working solution, and each sample tube requires anywhere from 180–199 µL. Therefore prepare a sufficient Qubit working solution to accommodate all standards and samples.
?. [Create Sample Dilution for Analysis]
Dilute sample 1:50 ( dH2O +  sample)
196 µl
4 µl
After taking into consideration the Qubit dilution factor (1:20; 10 μL sample + 190 μL qubit assay buffer) the sample being analyzed has been diluted 1:1000. This should give a value in the linear range for qubit (1.25-25 μg/mL). The dilution is also important to reduce the impact of SDS and 2-mercaptoethanol on quantification. The concentration of SDS must be >0.2 %, and 2-mercaptoethanol > 20 mM in the 10 μL sample added to the assay. The concentration of SDS in PEB is 2 %, and 2-mercaptoethanol 335 mM, so diluted 1:50 yields [SDS] 0.04 % and [2-mercaptoethanol] 6.7 mM, which is in the acceptable range.
?. [Create Sample Dilution for Analysis]
Add  of Qubit working solution to a fresh Qubit assay tube, one for each sample to be analyzed (including the three protein standards)
190 µl
?. [Create Sample Dilution for Analysis]
Add  of protein standard to the appropriate tube and mix by vortexing s.
10 µl
?. [Create Sample Dilution for Analysis]
Add  of diluted sample to the appropriate tube and mix by vortexing  s.
10 µl
?. [Create Sample Dilution for Analysis]
Allow samples to incubate at room temperature for
?. [Create Sample Dilution for Analysis]
Measure protein sample concentration using the Qubit, following the instructions on the machine (i.e. start by measuring the standard curve).
Remember to adjust measured values according to the dilution factor applied. In this example, after taking into consideration the Qubit dilution factor (1:20;   sample +    Qubit assay buffer) the sample has been diluted 1:1000
TITLE: DNA extraction protocol
AUTHORS: Unai Baroja, Inazio Garin, Joxerra Aihartza, Aitor Arrizabalaga-Escudero, Nerea Vallejo, Miren Aldasoro, Urtzi Goiti

[STEPS]
?. Heat the tubes for 15 minutes at in the heatblock
65 °C
Meanwhile add  C2 solution to empty 1.5 ml tubes and label them(for step 7)
Meanwhile add   C3 solution to empty 1.5 ml tubes and label them(for step 10)
?. Add the pellets to sterilised weigh trays and weigh the sample (note down numberof pellets + total weight)
?. Add the pellets to the labelled Dry Bead Tubes with Bead Solution and give a quick spin
?. Add  of preheated C1 solution to each Dry Bead Tube and vortex thoroughly (15 sec)
60 µl
?. Tissuelyse the tubes 10 minutes at freq 20 (2x20) in Precellys Tissue Homogenizer.
?. Centrifuge the tubes 13,000g for 3 minutes
?. Transfer the supernatant () to the 1.5 ml tubes containing C2 solutionand vortex briefly
450 µl
?. Incubate for 5 minutes at
4 °C
Meanwhile label N spin filters and 2N collection tubes
?. Centrifuge the tubes 13,000g for 1 minute
?. Transfer the supernatant (up to ) to the 1.5 ml tubes containing C3solution and vortex briefly
600 µl
?. Incubate for 5 minutes at
4 °C
Meanwhile label N low-binding 1.5 ml tubes with sample names/numbers
?. Centrifuge the tubes 13,000g for 1 minute
?. Transfer the supernatant (up to) to the 2 ml Collection Tubes
750 µl
?. Shake the C4 solution, add (550+550) to the supernatant and vortexbriefly
1100 µl
?. Load  onto a Spin Filter, centrifuge 8,000g for 1 minute and discard theflowthrough to a 50ml falcon tube
650 µl
?. Repeat the step 15 until loading all the mix
?. Add of C5 solution and centrifuge at 13,000g for 1 minute. Check if theliquid spun through and discard the flowthrough
500 µl
Avoid splashing C5 solution onto the Spin Filter!
?. Centrifuge again at 13,000g for 1 minute and place the Spin Filter in a clean 2 ml Collection Tube
?. Add 50 ul C6 solution to the center of the Spin Filter and incubate at  15 minutes
37 °C
?. Centrifuge 13,000g for 1 minute and discard the Spin Filter
?. Transfer the extract to 1.5 low bind tubes and distribute the extracts in PCR strips
TITLE: Prodentim Australia, NZ, Ireland, Canada or UK
AUTHORS: Prodentim  Australia

[DESCRIPTION]
ProDentim supplements include several ingredients that work on the mouth, tooth, gum, and breath. In addition, the components are beneficial for the whole body. 

ProDentim works on many levels to improve dental health gradually and effectively. It aids in the removal of most tooth and gum problems and conditions such as tartar, cavities, and plaque. It works very well due to the specially selected components. 

These substances work well because they have been combined in the right proportions. In the preparation, the manufacturer did not use any artificial additives or dangerous substances. 

Prodentim Official Web Click Here :-

https://www.outlookindia.com/outlook-spotlight/prodentim-reviews-australia-nz-2022-scam-or-legit-prodentim-canada-price-buy-in-prodentim-uk-ireland-or-united-states-news-211409/amp


[STEPS]