eamag/protocols-with-steps huggingface dataset:
TITLE: Preparação para ir ao campo
AUTHORS: Alicia Rafaelly Vilefort Sales, Daniel Vartanian, Maria Augusta Medeiros Andrade

[DESCRIPTION]
Este protocolo foi desenhado para o processo de coleta de dados do projeto de pesquisa Associações entre a duração e a qualidade do sono de gestantes no terceiro trimestre com a duração do trabalho de parto. Consulte a aba Guidelines para visualizar o fluxograma do processo como um todo.

Uma coleção com todos os protocolos do processo deve ser publicada em breve.

Caso tenha dúvidas ou sugestões, entre em contato com um dos autores.

---

This protocol was designed for the data collection process of the research project Associations between the duration and quality of sleep of pregnant women in the third trimester with the duration of labor. See the Guidelines tab to view the process flowchart as a whole.

A collection with all the process protocols should be published soon.

If you have any questions or suggestions, please contact one of the authors.

[BEFORE_START]
Este protocolo assume que o(a) usuário(a) se encontra na sala do Grupo Interdisciplinar de Pesquisa em Sono (GIPSO). Os materiais citados neste documento já devem estar à disposição.

Entre em contato com a supervisão do projeto caso encontre algum problema.

[GUIDELINES]
O processo de coleta de dados deste projeto segue o seguinte fluxograma.

  
 Com exceção da etapa Processe e analise os dados, todas as etapas possuem protocolos próprios.

[STEPS]
SECTION: Outros materiais
6. Separe os itens abaixo conforme a quantidade de gestantes que irá recrutar, sempre deixando algumas cópias extras por segurança.

 

Termo de Consentimento Livre e Esclarecido  (TCLE)

 

 Formulário de campo

 

Termo de entrega de actígrafo

 

Guia de instruções e cuidados com o actígrafo
Formulário de controle

SECTION: Aparelho celular
8. Certifique-se que você tem acesso à internet pela sua operadora de telefonia.

 

SECTION: Ida ao campo
10. Siga para o recrutamento de voluntárias na casa de parto.

 

SECTION: Actígrafos
5. Armazene os actígrafos de maneira segura.

 

SECTION: Actígrafos
4. Realize o processamento pré-coleta dos actígrafos.

 

SECTION: Agenda
1. Consulte a agenda do projeto e verifique a quantidade de gestantes a serem recrutadas no seu turno.

 

SECTION: Actígrafos
3. Separe um actígrafo para cada gestante a ser recrutada e mais um actígrafo extra por segurança.

 

SECTION: Outros materiais
7. Separe também os seguintes itens:

1 Folha de acesso
3 canetas esferográficas de cor azul ou preta
1 Porta crachá
1 Prancheta

 

SECTION: Agenda
2. Verifique os horários e o trajeto que você terá que fazer para que você chegue com ao menos 30 minutos de antecedência na Casa de Parto.
SECTION: Aparelho celular
9. Certifique-se que seu celular está com 100% de bateria.
TITLE: Italian set up of the program “REsilience and Activity every DaY for MS”, of outcomes, and pilot assessment of efficacy using a mixed methodology (READY-It-MS)
AUTHORS: Ambra Mara Giovannetti, Alessandra Solari, Kenneth Pakenham

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Italian set up of the program “REsilience and Activity every DaY for MS”, of outcomes, and pilot assessment of efficacy using a mixed methodology (READY-It-MS)</div></div>

[STEPS]
TITLE: Domestication of L0 parts for Loop type IIS (BsaI and SapI)
AUTHORS: Eftychis Frangedakis, marta tomaselli, Marius Rebmann, Susana Sauret-Gueto

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Domestication and cloning of L0 parts into pUAP4 for </span><a href="https://www.protocols.io/view/loop-l1-odd-level-bsai-type-iis-cloning-into-pck-v-92gh8bw" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;">Loop type IIS assembly</span></a></div></div>

[STEPS]
?. [PCR and amplified fragments purification   ]
Amplify the parts using your preferred polymerase (e.g. Phusion, Thermo Scientigic™) following the manufacturer instructions.
?. [Domestication Guide.]
?. [PCR and amplified fragments purification   ]
Run your PCR product on agarose gel.
?. [Fragments Assembly]
Set up the assembly of the fragments as in cloning of L0 parts into pUAP4[pUAP4]= plasmid length/200  [fragment]= fragment length/100The formulas above will give you the required concentration in ng/µl.
?. [PCR and amplified fragments purification   ]
Extract the amplified fragments using a PCR purification kit (e.g. Qiagen MinElute™ ).
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for more information regarding this protocol.</p>
<p> </p>
<p>Modified from Pardee, A. B., F. Jacob, and J. Monod. 1959. The genetic control and cytoplasmic expression of "inducibility" in the synthesis of ß-galactosidase in E. coli. J. Mol. Biol. 1:165-178</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Preparation of Frozen Bacteria Stock
AUTHORS: Kenneth Schackart

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol details how to prepare bacteria culture for deep freezing. Protocol adapted from the protocol provided as an external link.</div><div class = "text-block">Reference:</div><div class = "text-block"><span>Vitko N.P. and Richardson A.R. 2013. Laboratory Maintenance of Methicillin-Resistant Staphylococcus aureus (MRSA). </span><span style = "font-style:italic;">Curr Protoc Microbiology</span><span>. Basic Protocol 4: Preparation of </span><span style = "font-style:italic;">S. aureus</span><span> Frozen Stocks.</span></div></div>

[STEPS]
?. [Prepare Stocks]
Add  sterile DMSO to sterile cryotube.
150 µl
?. [Prepare Stocks]
Add  overnight culture to cryotube containing.
850 µl
?. [Prepare Stocks]
Vortex or invert repeatedly to mix.
?. [Prepare Stocks]
Store cryotube in deep freezer ( to  )
-40 °C
-80 °C
?. [Update Inventory]
Go to the frozen storage inventory and add at a minimum the following information:Storage locationYour nameDateOrganism identification
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<div>
<div>
<div>
<div>
<div>
<p>This protocol was designed and developed at this laboratory.</p>
<p>The protocol specifically aims to amplify strains of Influenza B YAMAGATA virus lineage and not strains of the VICTORIA virus lineage or other virus species. The assay targets the haemagglutinin (HA) region and is designed as a qualitative lineage-typing test for human cases of seasonal influenza virus type B infections.</p>
<p>FluB-YAMA-TM2018 is ideally used alongside its companion protocol, "Influenza B virus VICTORIA lineage TaqMan 2018" (FluB-VICT-TM2018), which aims to target influenza B virus VICTORIA lineage strains exclusively. The two assays perform best as UNIPLEX protocols; a drop in sensitivity was observed when combined in a DUPLEX format. </p>
</div>
</div>
</div>
</div>
</div>

[BEFORE_START]
<div>
<div>
<div>
<div>
<div>
<div>
<ul>
<li>If using a different brand or model of real-time thermocycler, check the concentration of ROX is adequate.</li>
<li>Method assumes the user is familiar with the thermocycler and software used to run the protocol and with PCR in general.</li>
</ul>
</div>
</div>
</div>
</div>
</div>
</div>

[STEPS]
?. 
?. 
?. 
?. 
?.
TITLE: Donor Case Selection | HuBMAP | JHU-TMC
AUTHORS: Kyu Sang Han, Joel Sunshine, Sashank Reddy, Denis Wirtz, Pei-Hsun Wu

[DESCRIPTION]
Donor case selection protocol for Johns Hopkins TMC describes criteria for human tissue biopsy collection.

[BEFORE_START]
Patient samples are obtained from skin that is to be discarded from patients presenting for reconstructive or aesthetic procedures necessitating skin excision. No other procedures or follow-up is needed for research purposes.

[STEPS]
SECTION: Inclusion
1. Age 18-35 or 50 and over
English Speaking
Able to provide consent
Undergoing reconstructive or aesthetic procedures necessitating skin excision at the scalp or abdomen
SECTION: Exclusion
2. Known collagen disorder
Strong personal history of keloid formation
Deemed unfit for general anesthesia
Active infection of skin where samples may be taken
TITLE: Protocols from 237 CAR-T studies in a mouse ovarian tumor model (reported in Ranoa et al JITC 2023)
AUTHORS: Diana Rose E Ranoa, Preeti Sharma, Claire P. Schane, Amber N Lewis, Edward Valdez, Edward J. Roy, David M. Kranz

[DESCRIPTION]
This is a collection of protocols from the manuscript: Ranoa, D. R. E.; Sharma, P.; Schane, C.P.; Lewis, A.N.; Valdez, E.; Marada, V. V. V. R.; Hager, M.; Montgomery, W.; Wolf, S. E.; Schreiber, K.; Schreiber, H.; Bailey, K.; Fan, T. M.; Hergenrother, P. J.; Roy, E. J.; Kranz, D. M. A single CAR-T cell treatment controls disseminated ovarian cancer in a syngeneic mouse model. Submitted to Journal for ImmunoTherapy of Cancer 2023; doi:10.1136/jitc-2022-006509.

[STEPS]
TITLE: Nuclei isolation from human intestinal biopsic tissue for single-cell genomic applications
AUTHORS: Ran Zhou, Oni Basu

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol provides an efficient method to isolate nuclei from human intestinal biopsy samples for single cell applications (RNA-seq or ATAC-seq).</div></div>

[STEPS]
?. [Sample preparation]
Rinse fresh samples in ice-cold PBS twice.
Biopsy tissuse can be store up to 3 days in liquid nitrogen/at - 80 Celsius following the steps below:Tissues are rinsed in ice-cold PBS twiceFlash freeze the tissue in 1.7 ml Eppendorf tube in liquid nitrogenStore frozen tissue in liquid nitrogen (preferred) or at -80CStart from step 2 if working with frozen tissues.
?. [Tissue lysis]
Mince the tissue, with 200 ul lysis buffer added, in a 1.7 ml Eppendorf tube by Iris Scissors on ice x 1 mins.
?. [Tissue lysis]
Add 1-1.5 ml ice-cold lysis buffer to the tube and incubate on ice x 5 mins. Invert the tube 3 times in the middle of the incubation to mix.
?. [Tissue lysis]
Wet a 40 micron cell strainer with 1 ml lysis buffer.
?. [Tissue lysis]
Filter the lysis through the strainer. Wash the strainer by 3 ml lysis buffer and 4 ml nuclei suspension buffer (NSB). Keep the flow through as this is where your nuclei are.
?. [Nuclei collection]
Spin down the flow-through, at 600 g x 5 mins, at 4 celsius in a 15 ml conical tube.
Centrifuge: 600 34, 5 min, 4 10
?. [Nuclei collection]
Suspend nuclei in 100 ul NSB using gentle pipetting.
If working with a single-cell platform, e.g. 10x Genomics, the nuclei should be suspended in PBS + 1% BSA + 0.2 U/ul RNase Inhibitor
?. [Nuclei collection]
Take 10 ul nuclei suspension and mix with 10 ul DAPI or Hoechst dye at 10 ug/ml and 10 ul WGA dye at 1 ug/ml. Count the nuclei.
?. [Nuclei preparation]
Dilute nuclei to the desired density using NSB.
?. [Nuclei collection]
If red bloold cells are present in fresh tissue nuclei suspension, dilute the suspension with NSB to 1 ml. Add 2 ml RBC lysis buffer and incubate on ice for 5 minutes. Pellet nuclei by centrifugation 600 g x 5 mins, 4 Celsius. Suspend nuclei in 100 ul NSB with gentle pipetting.
Centrifuge: 600 34, 5 min, 4 10
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Sirius Red staining is a usefull staining to study collagen distribution. In adipose tissue development during obesity the extracellular matrix is being remodeled and this can be analyzed using Sirius Red staining. In addition, fibrosis in livers from mice suffering from non-alcoholic steatohepatitis can be identified using this staining. Moreover, scar formation after the induction of a myocardial infarct can be illustrated using the Sirius Red staining.</p>

[GUIDELINES]
<p>To weigh off Picric acid, the entire bottle is weighed before and after some Picric acid is transfered to another recipient.</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Cartilage staining
AUTHORS: Satheeswaran Balasubramanian, Ekambaram Perumal

[DESCRIPTION]
The Alcian blue staining technique is widely used among developmental biologists to observe the development of cartilage and bone in zebrafish embryos and larvae. Alcian blue is a positively charged dye that stains the cartilage through an electrostatic interaction with negatively charged acidic mucopolysaccharides in the presence of magnesium ions (Scott et al. 1996).

[BEFORE_START]
Every protocol needs to be validated in the laboratory when first introduced. The present protocol describes validation steps that were taken in the Molecular Toxicology lab, at Bharathiar University. 
Always wear personal protective equipment. 
All the steps are optimized in a 24-well plate for multiple groups such as control and treated. 

[GUIDELINES]
This protocol is optimized for 5 days post-fertilization (dpf) of zebrafish larvae. It can be adapted for higher dpf.

[STEPS]
SECTION: Larval fixation
1. At the desired stage, take the zebrafish larvae and wash in PBS for 5 minutes.
SECTION: Larval fixation
2. Euthanize the larvae using the cold shock method (Keep at 4oC for 5-10 minutes).
SECTION: Larval fixation
3. Transfer the euthanized larvae into 4% PFA and keep it in the rocker for 2 hours at room temperature (if required, it can be kept overnight at 4oC).
SECTION: Larval fixation
4. Wash the fixed larvae with PBST for 5 minutes, two times.
SECTION: Larval fixation
5. Dehydrate the larvae using 50% ethanol and keep it in the rocker for 10 minutes at room temperature.
SECTION: Staining
6. Add 1 ml of alcian blue staining solution to the dehydrated larvae and keep it in a rocker overnight at room temperature.
SECTION: Bleaching
7. Rinse the stained larvae once using PBST.
SECTION: Bleaching
8. Transfer the larvae to a bleach solution and incubate in dark at room temperature. Assess the bleaching process (using a microscope) and stop the reaction when the larvae become transparent (approximately 20-30 min for 4 dpf and above).
SECTION: Clearing
9. Remove the bleach solution and rinse once using PBST.
SECTION: Clearing
10. Clear the beached larvae with 25% glycerol and 0.25% KOH. Keep them in a rocker at room temperature for 30 minutes to overnight.
SECTION: Clearing
11. Replace the solution with 50% glycerol and 0.25% KOH. Keep them in a rocker at room temperature for 2 hours to overnight.
SECTION: Storage
12. Store larvae in a solution of 50% glycerol and 0.1% KOH at 4oC. Avoid long-term storage as it will diminish the stain. Capture images of the cartilage using any bright field microscope (preferably a stereo microscope). 
SECTION: Results
13.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>To control a disease it is essential to know its causal agent. A correct diagnosis provides a considerable amount of basic information to select correct control methods. An accurate diagnosis always starts with a representative sample of the damaged tissue, from which a portion is taken, to isolate the causal agent. In the case of phytopathogenic fungi, there are several isolation methods, depending on the tissue or substrate in which they are found. This protocol describes the isolation and purification of phytopathogenic fungi from leaves and fruits of different tropical crops.</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Plasmid maps and genbank files
AUTHORS: Tamara Matute, Isaac Nuñez, Fernan Federici, Peter von Dassow

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Plasmid maps and genbank sequence files for the plasmids used in the transformation of </span><span style = "font-style:italic;">Nannochloropsis oceanica</span><span> and environmental samples following the protocol </span><a href="https://www.protocols.io/view/fabrication-of-dna-constructs-by-gibson-assembly-a-7r8hm9w" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;">Matute et al.</span></a><span style = ":;">.</span></div></div>

[STEPS]
?. 
?.
TITLE: Small Scale IPTG Induction | Protein Expression
AUTHORS: Catherine M Gohar

[DESCRIPTION]
Test

[BEFORE_START]
It's best to start small-scale protein purification on a Monday or Tuesday. This protocol will take at least 3 days before the first freezing step.

[STEPS]
SECTION: Seed Culture
4. Take the BL21 glycerol stock from the -80 °C freezer and place immediately into the ice bucket.

SECTION: Seed Culture
2. Label a 15 mLculture tube with the vector strain you will be using, and fill with 5 mL  of LB broth with 1x Kanamycin antibiotic or 5ul of 1000x.

SECTION: Seed Culture
5. Use a 200 µL pipette tip to scrape some of the ice from the tube, and place the tip directly into the culture tube with LB.

SECTION: Seed Culture
1. Preheat the shaking tube incubator to 37 °C 

SECTION: Seed Culture
3. Place the labeled tube into the shaking incubator to warm, then grab an ice bucket and fill with ice.
SECTION: Seed Culture
6. Let the culture shake at 37 °C overnight to be completely saturated by the next morning.

SECTION: Culture Expansion
7. Grab a sterilized 250 mL Erlenmeyer flask and fill with 45mL of Terrific Broth and 1x Kanamycin antibiotic.

SECTION: Culture Expansion
8. Prewarm the flask in the shaking tube incubator at 37 °C 

SECTION: Culture Expansion
9. Add all 5 mL of the seed culture to the Erlenmeyer flask. The culture should expand 1:10 in 1-2 hours, to an OD around 0.4-0.6.

SECTION: Culture Expansion
10. Thaw a stock of IPTG on ice about 30 minutes before you suspect the culture will be at 0.4 OD.
SECTION: Culture Expansion
11. Quantify the OD of your 50 mL flask using 500 µL of culture in a spectrometer cuvette. Be sure to blank with 500 µL of Terrific Broth.

SECTION: Culture Expansion
12. Once OD hits 0.4-0.6, preheat the fridge shaking incubator to 16 °C 

SECTION: Culture Expansion
13. Dilute1 Molarity (M) IPTG  stock to the appropriate concentration for protein induction by adding to expanded culture. This will most likely be a range of 100 micromolar (µM) to 1 millimolar (mM)

SECTION: Culture Expansion
13.1. To calculate IPTG concentration,  use V1 x C1 = V2 x C2, where column E is equivalent to V2.

 

SECTION: Culture Expansion
14. Take your IPTG-induced culture and shake overnight at 16 °C for about 16-18 hours.
SECTION: Pellet Cells
15. Take each 50 mL culture and pour into a 50 mL conical tube, labelled.

SECTION: Pellet Cells
16. Take the cells to a large or spinning-bucket centrifuge and spin at   

SECTION: Pellet Cells
17. Decant cells into a bleach waste bin labeled for culture, and dry on a paper towel for 5-10 seconds
SECTION: Pellet Cells
18. Freeze the pellet at -80 °C by storing in a freezer for storage, or continue to the protocol for cell lysis.
TITLE: XP Nutrition Keto Gummies Review: Shocking Side Effects?
AUTHORS: xpnutritionketoreview  

[DESCRIPTION]
XP Nutrition Keto Gummies Review: Shocking Side Effects?


[STEPS]
1. XP Nutrition Keto Gummies Review: Shocking Side Effects?
Are you looking for a quick, easy, and effective way to exfoliate that redundant body fat in a short period of time? Do you want to feel more and perform better? Also it’s time to try XP Nutrition Keto Gummies. You won't find a better ketogenic diet supplement anywhere. 
What are XP Nutrition Keto ACV Gummies? 
XP Nutrition Keto ACV Gummies is a revolutionary new salutary supplement that combines the stylish of high- quality constituents that have been proven to work together to help you lose weight presto and keep it off. This supplement contains natural and organic constituents that are precisely formulated to support your healthy ketogenic diet and help you lose weight naturally. 
It'll help you lose weight, lower cholesterol, burn fat, boost energy situations, and more. They can work as a standalone product, or you can mound them with other supplements to get indeed lesser results. 
How Does XP Nutrition Keto ACV Gummies Work? 
The way in which XP Nutrition ACV Gummies work is fairly simple. They encourage the body to enter a state of ketosis, a metabolic state associated with a lower appetite. The idea is that when you ’re in a state of ketosis, you ’ll naturally eat less and lose weight. This is because when the body is in ketosis, it releases hormones that make you feel full and satisfied and can lead to a reduction in appetite and an overall reduction in calorie input. 
When you eat a mess containing carbs, the body converts the carbs into glucose, a form of sugar that's absorbed through the digestive tract. Glucose is also transported throughout the body, where it’s used as energy. This is why keto-friendly foods are also appertained to as keto-friendly, or fat-burning, foods. 

Official Website:- http://softtechfreak.com/health/xp-nutrition-keto-gummies-reviews-scam-or-legit/
TITLE: Tris-Buffered Saline (TBS)
AUTHORS: Andreas Sagen

[DESCRIPTION]
Tris-buffered saline (TBS) is a buffer used in certain biochemical techniques to keep the pH within a limited range. This formulation has an approximate sodium chloride concentration of 150 mM and a tris concentration of 50 mM. The pH of the buffer is adjusted to 7.6. TBS is frequently formulated with a detergent such as Tween-20 for staining applications such as Western blotting. As a surfactant, Tween-20's function is to dissolve the surface tension between the membrane and the solution, ensuring complete membrane surface area wetting.

[STEPS]
SECTION: 1 000 mL 25xTBS
1. Fill 800 mL distilled water in a bottle
SECTION: 100 mL TBS with 0.1% Tween-20 (TBST)
11. Fill 80 mL distilled water in a glass bottle
SECTION: 1 000 mL 25xTBS
5. Sterilize by autoclave for 40 min
SECTION: 1 000 mL 25xTBS
4. Fill bottle to 1000 mL with distilled water
SECTION: 1 000 mL 25xTBS
3. Adjust to pH 7.6 with 12N hydrochloric acid

Reagents:
 
SECTION: 1 000 mL 25xTBS
2. Measure and add:
150 g Trizma Base (NH2C(CH2OH)3; 121.14 g mol-1)
220 g Sodium Chloride (NaCl; 58.44 g mol-1)

Reagents:
 
  
SECTION: 100 mL TBS with 0.1% Tween-20 (TBST)
14. Fill bottle to 100 mL with distilled water
SECTION: 100 mL TBS with 0.1% Tween-20 (TBST)
12. Add 4 mL 25xTBS solution and 1 mL 10% Tween-20

 

Reagents:
  
SECTION: 1 000 mL 1xTBS
7. Fill 800 mL sterile water in a glass bottle
SECTION: 1 000 mL 25xTBS
6. Aliquot in 40 mL units, and store at -20 °C
SECTION: 100 mL TBS with 0.1% Tween-20 (TBST)
15. Sterilize with a 0.22 micron filter, and store at 4 °C
SECTION: 1 000 mL 1xTBS
10. Fill bottle to 1000 mL with sterile water, and store at 4 °C
SECTION: 1 000 mL 1xTBS
8. Add 40 mL 25xTBS solution
SECTION: 1 000 mL 1xTBS
9. Mix solids completely, then adjust pH to pH 7.6 with 12N hydrochloric acid

Reagents:
 
SECTION: 100 mL TBS with 0.1% Tween-20 (TBST)
13. Mix solids completely, then adjust pH to pH 7.6 with 12N hydrochloric acid

Reagents:
 
SECTION: 100 mL 5% Non-fat dry milk (NFDM) in 1xTBST
19. Measure 1 g BSA in a glass bottle

Reagents:
 
SECTION: 100 mL 5% Non-fat dry milk (NFDM) in 1xTBST
16. Measure 1 g Non-fat dry milk (NFDM) in a glass bottle

Reagents:
 
SECTION: 100 mL 5% Non-fat dry milk (NFDM) in 1xTBST
20. Fill bottle to 100 mL with 1xTBST
SECTION: 100 mL 5% Non-fat dry milk (NFDM) in 1xTBST
21. Sterilize with a 0.22 micron filter, and store at 4 °C
SECTION: 100 mL 5% Non-fat dry milk (NFDM) in 1xTBST
17. Fill bottle to 100 mL with 1xTBST
SECTION: 100 mL 5% Non-fat dry milk (NFDM) in 1xTBST
18. Sterilize with a 0.22 micron filter, and store at 4 °C
TITLE: The processing files and analysis result files
AUTHORS: XX  

[DESCRIPTION]
The base data for bivariate autocorrelation and geo-probe analysis and the Geodetector analysis results are included here. The GeoDA analysis results mentioned in the paper can be obtained by running the base data for bivariate autocorrelation analysis, and the data can be converted using the Geodetector sampling points and imported into the Geodetector software to obtain the analysis results provided. The specific operation of both analysis software can be learned in Ref.

[GUIDELINES]
All raw data are publicly available on the website mentioned in the paper. The provided files were mainly used to analyze the relationship between impact factors and habitat quality, and the operational steps are described in the paper.

[STEPS]