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[GUIDELINES]
<strong>Turk's Island Salt Mix</strong><br />
<table>
<tbody>
<tr>
<td style="text-align: center;">Chemical</td>
<td style="text-align: center;">g/2 L</td>
<td style="text-align: center;">Final Conc.</td>
<td style="text-align: center;">Mfc.-PN</td>
<td style="text-align: center;">Chemical Grade</td>
</tr>
<tr>
<td style="text-align: left;">&nbsp;NaCl</td>
<td style="text-align: center;">&nbsp;56.22</td>
<td style="text-align: center;">481 mM&nbsp;</td>
<td style="text-align: center;">&nbsp;Fisher S271</td>
<td style="text-align: center;">ACS&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">MgSO<sub>4</sub>&middot;7H<sub>2</sub>O&nbsp;</td>
<td style="text-align: center;">13.8&nbsp;</td>
<td style="text-align: center;">28 mM&nbsp;</td>
<td style="text-align: center;">Sigma M2773&nbsp;</td>
<td style="text-align: center;">Mo.Biol. 99%&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">MgCl<sub>2</sub>&middot;6H<sub>2</sub>O&nbsp;</td>
<td style="text-align: center;">10.98&nbsp;</td>
<td style="text-align: center;">27 mM&nbsp;</td>
<td style="text-align: center;">AlfaAesar&nbsp;</td>
<td style="text-align: center;">99.99%&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">CaCl<sub>2</sub>&middot;2H<sub>2</sub>O&nbsp;</td>
<td style="text-align: center;">2.94&nbsp;</td>
<td style="text-align: center;">10 mM&nbsp;</td>
<td style="text-align: center;">Fisher C79&nbsp;</td>
<td style="text-align: center;">ACS&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">KCl&nbsp;</td>
<td style="text-align: center;">1.34&nbsp;</td>
<td style="text-align: center;">9 mM&nbsp;</td>
<td style="text-align: center;">Acros&nbsp;</td>
<td style="text-align: center;">99+%&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">MQ&middot;H<sub>2</sub>O&nbsp;</td>
<td style="text-align: center;">QS to 2000 ml&nbsp;</td>
<td style="text-align: center;">na&nbsp;</td>
<td style="text-align: center;">in house&nbsp;</td>
<td style="text-align: center;">na&nbsp;</td>
</tr>
</tbody>
</table>
<br /><br /><strong>Macronutrients</strong>&nbsp;<br />
<table>
<tbody>
<tr>
<td style="text-align: center;">Macronutrient&nbsp;</td>
<td style="text-align: center;">g/100 ml&nbsp;</td>
<td>Final Conc.</td>
<td style="text-align: center;">Mfc.-PN&nbsp;</td>
<td style="text-align: center;">Chemical Grade&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">0.1 M NaH<sub>2</sub>PO<sub>4</sub>&middot;H<sub>2</sub>O</td>
<td style="text-align: center;">1.38&nbsp;</td>
<td style="text-align: center;">50 &micro;M&nbsp;</td>
<td style="text-align: center;">Mallinckrodt 7892-04&nbsp;</td>
<td style="text-align: center;">ACS&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">0.8 M (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>&nbsp;</td>
<td style="text-align: center;">10.57&nbsp;</td>
<td style="text-align: center;">400 &micro;M&nbsp;</td>
<td style="text-align: center;">VWR-BDH 0216&nbsp;</td>
<td style="text-align: center;">ACS 99%&nbsp;</td>
</tr>
</tbody>
</table>
<br /><strong>Buffers</strong><br />
<table>
<tbody>
<tr>
<td style="text-align: center;">Buffer</td>
<td style="text-align: center;">Stock</td>
<td style="text-align: center;">Final Conc.</td>
<td style="text-align: center;">Mfc.-PN</td>
<td style="text-align: center;">Chemical Grade</td>
</tr>
<tr>
<td style="text-align: left;">0.6 M NaHCO<sub>3</sub></td>
<td style="text-align: center;">0.504 g/10 ml&nbsp;</td>
<td style="text-align: center;">6 mM&nbsp;</td>
<td style="text-align: center;">Sigma S6014</td>
<td style="text-align: center;">ACS 99.7-100.3%&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">1 M HEPES&nbsp;</td>
<td style="text-align: center;">11.9 g/50 ml&nbsp;</td>
<td style="text-align: center;">1 mM&nbsp;</td>
<td style="text-align: center;">Fisher BP310&nbsp;</td>
<td style="text-align: center;">Mol. Biol. &nbsp;</td>
</tr>
</tbody>
</table>
<br /><strong>Trace Metal Mix Working Stock</strong><br />
<table>
<tbody>
<tr>
<td style="text-align: center;">Trace Metal</td>
<td style="text-align: center;">Primary Stock</td>
<td style="text-align: center;">Final Conc.</td>
<td style="text-align: center;">Mfc.-PN</td>
<td style="text-align: center;">Chemical Grade</td>
</tr>
<tr>
<td style="text-align: left;">Na<sub>2</sub>EDTA&middot;2H<sub>2</sub>O&nbsp;</td>
<td style="text-align: center;">0.2175 g/50 ml</td>
<td style="text-align: center;">0.1170 &micro;M&nbsp;</td>
<td style="text-align: center;">Sigma E4884&nbsp;</td>
<td style="text-align: center;">ACS 99-101%</td>
</tr>
<tr>
<td style="text-align: left;">FeCl<sub>3</sub>&middot;6H<sub>2</sub>O</td>
<td style="text-align: center;">0.16 g/50 ml&nbsp;</td>
<td style="text-align: center;">0.1180 &micro;M&nbsp;</td>
<td style="text-align: center;">Sigma 44944</td>
<td style="text-align: center;">ACS 98-102%&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">ZnSO<sub>4</sub>&middot;7H<sub>2</sub>O&nbsp;</td>
<td style="text-align: center;">1.15 g/50 ml&nbsp;</td>
<td style="text-align: center;">0.0008 &micro;M&nbsp;</td>
<td style="text-align: center;">Sigma 204986&nbsp;</td>
<td style="text-align: center;">&gt;99.5%&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">CoCl<sub>2</sub>&middot;6H<sub>2</sub>O&nbsp;</td>
<td style="text-align: center;">0.595 g/50 ml&nbsp;</td>
<td style="text-align: center;">0.0005 &micro;M&nbsp;</td>
<td style="text-align: center;">Sigma 60820&nbsp;</td>
<td style="text-align: center;">&gt;98%&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">MnCl<sub>2</sub>&middot;4H<sub>2</sub>O&nbsp;</td>
<td style="text-align: center;">8.905 g/50 ml&nbsp;</td>
<td style="text-align: center;">0.0090 &micro;M&nbsp;</td>
<td style="text-align: center;">M3634&nbsp;</td>
<td style="text-align: center;">ReagentPlus &gt;99%&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">Na<sub>2</sub>MoO<sub>4</sub>&middot;2H<sub>2</sub>O&nbsp;</td>
<td style="text-align: center;">0.363 g/50 ml&nbsp;</td>
<td style="text-align: center;">0.0003 &micro;M&nbsp;</td>
<td style="text-align: center;">M1651&nbsp;</td>
<td style="text-align: center;">&gt;99.5%&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">Na<sub>2</sub>SeO<sub>3</sub>&nbsp;</td>
<td style="text-align: center;">0.865 g/50 ml&nbsp;</td>
<td style="text-align: center;">0.0010 &micro;M&nbsp;</td>
<td style="text-align: center;">S5261&nbsp;</td>
<td style="text-align: center;">BioReagent ~98%&nbsp;</td>
</tr>
<tr>
<td style="text-align: left;">NiCl<sub>2</sub>&middot;6H<sub>2</sub>O&nbsp;</td>
<td style="text-align: center;">1.19 g/50 ml&nbsp;</td>
<td style="text-align: center;">0.0010 &micro;M&nbsp;</td>
<td style="text-align: center;">223387&nbsp;</td>
<td style="text-align: center;">ReagentPlus&nbsp;</td>
</tr>
</tbody>
</table>
Note: Use primary stocks of ZnSO<sub>4</sub>, CoCl<sub>2</sub>, MnCl<sub>2</sub>, Na<sub>2</sub>MoO<sub>4</sub>, Na<sub>2</sub>SeO<sub>3</sub>, and NiCl<sub>2</sub> prepared for Pro99 trace metal working stock.<br /><br /><img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/vqc2xe.jpg" alt="" data-src="https://s3.amazonaws.com/pr-journal/vpc2xe.jpg" data-ofn="IMG_3559.JPG" /><br /><img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/vsc2xe.jpg" alt="" data-src="https://s3.amazonaws.com/pr-journal/vrc2xe.jpg" data-ofn="IMG_3560.JPG" /><br /><br />

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