TITLE: Hydra collecting for citizen scientists
AUTHORS: Callen  Hyland, Kimberly Sladek

[DESCRIPTION]
The freshwater cnidarian Hydra has been a model system for regeneration and developmental biology for over 250 years, but much remains unknown about their biodiversity and global distribution. As a citizen scientist, you can contribute to our understanding of Hydra in the wild by becoming a "Hydra Hunter". All it takes is a few simple materials and a little patience.

Collecting Hydra in the wild can be challenging. You will certainly not find them everywhere you look. Keep in mind that NOT finding Hydra is still useful information because this will help us understand the environmental factors that effect their distribution.

Metadata submission form: https://forms.gle/cAZCiiRCyE922G5t5

Please contact chyland@sandiego.edu for more information or to receive a Hydra collecting kit.

Hydra collecting kits were purchased with a grant to Kimberly Sladek from the University of San Diego Associated Students Government.

Thank you to Rob Steele for helpful feedback on this protocol.


References:

Campbell, R. D. (1983). Hydra Collecting. In H. M. Lenhoff (Ed.). Hydra: Research Methods. New York: Springer Science + Business Media.

Martínez, D. E., et al. (2010). Phylogeny and biogeography of Hydra  (Cnidaria: Hydridae) using mitochondrial and nuclear DNA sequence. Molecular Phylogenetics and Evolution, 57, 403-410. doi:10.1016/j.ympev.2010.06.016

[BEFORE_START]
Make sure you have all collecting equipment and are prepared for a day outside in the field.
Familiarize yourself with the metadata submission form so you know what to keep track of when you are in the field.

If it will be a long walk to get to the collecting site, bring water, sunblock, and appropriate clothing for changing weather conditions. Be cautions around fast flowing streams, deep water, and slippery rocks.

Research your collecting locations before starting. Avoid private property and restricted areas. You are responsible for obtaining all necessary permits and permissions before collecting. Respect the environment by avoiding sensitive habitats and by disturbing vegetation and wildlife as little as possible.

[STEPS]
SECTION: Collecting Hydra
1. Gather your materials:
Notebook and pen
White or clear plastic container (a medium-sized food storage container is ideal)
Glass Pasteur pipette + rubber bulb
Screw-cap plastic tubes
Magnifying glass, high powered reading glasses, or headband magnifier
Optional: plastic toothbrush holder to protect your glass pipettes

The Hydra Collecting Kit contains:
Plastic food storage container
Two glass Pasteur pipettes + one rubber bulb
Credit card-sized magnifying glass
Three plastic screw-cap tubes
SECTION: Collecting Hydra
2. Familiarize yourself with the appearance and anatomy of Hydra so you know what you are looking for.

Check out Hydra observations on iNaturalist for beautiful images of the many different ways that Hydra may appear in its natural environment.

  
 

SECTION: Collecting Hydra
3. Select your collecting location.
Hydra are found in fresh water bodies all over the world. Look for ponds, lakes, rivers, and streams with accessible shoreline. Especially promising areas will have slowly moving water, abundant small invertebrates, and water plants to which Hydra can attach.

    
SECTION: Collecting Hydra
4. Once you are at the collecting site, record metadata about the environment:
Name of the location
GPS coordinates
Date and time
Brief description of the location
Any wildlife observed
Human activity observed
Description of the weather including air and water temperature. You can measure it if you have a thermometer, otherwise provide an estimate.
 

 

You can access the metadata submission form with your mobile device and fill it out while you are in the field, or you can write everything down in your notebook and fill out the form when you get home.
https://forms.gle/cAZCiiRCyE922G5t5
SECTION: Collecting Hydra
5. Hydra attach to submerged surfaces (rocks, sticks, leaves, reeds, stems of water plants, shells) through their basal disk, so this is likely where you will find them in the water. Fill your plastic container with water, then pick up various items from the water and place them in your container. Use your magnifier to examine every side looking for Hydra. If there are none, put the items back into the water and repeat.

    

Hydra can be difficult to spot since they are less than a centimeter long. It can be especially challenging if there is algae on the surfaces you are examining. Here are some tips for what to look for. Add a comment if you have additional suggestions.

Hydra may be extended or contracted. If extended you will notice the cylindrical body column. If contracted, they will appear spherical on the surface of the item you are examining. Either way, they will look more "geometrical" than the surrounding algae and debris.
Look for fine tentacles extending up into the water column.
Hydra can be either green or brown. In the author's experience, green Hydra are much darker green than surrounding algae and aquatic plants. Brown Hydra look tan or translucent.

   
SECTION: Collecting Hydra
6. Did you find Hydra? If so, use the Pasteur pipette to fill a screw-cap plastic tube with water, then use the Pasteur pipette to scrape the Hydras off the surface and then suck them up and transfer them into the tube.
Record the number of Hydra you found at each location.

     
 

SECTION: Collecting Hydra
7. Make sure to fill out the metadata submission form for every site you visit. Fill out a separate form for each site, even places where you did not find Hydra (under "number of Hydra found", record 0 if you did not find any). https://forms.gle/cAZCiiRCyE922G5t5

If you have collected Hydra, contact chyland@sandiego.edu for the next steps.
SECTION: Shipping Hydra
9. If possible, collect water from the pond or stream where you found the Hydra, avoiding algae and debris. The water should be as clear as possible. If water from the source appears cloudy:

Try collecting water from close to the surface of the pond or stream.
Let the water sit undisturbed for a couple of hours to let debris settle.
Filter the water through a coffee filter, cloth napkin, or bandana.

If you don't have water from the original source, you can substitute commercial spring water (e.g. Arrowhead). Do not use tap water, drinking water, or distilled water.
SECTION: Shipping Hydra
10. Place each Hydra in a separate 5 mL screw-cap plastic tube. Fill the tube completely with water and seal it tightly leaving no air bubbles. Hydra can live for over a month in a tube with no oxygen, but can be damaged by air bubbles sloshing around.
SECTION: Shipping Hydra
11. Wrap the tubes in an absorbent material, like paper napkins or paper towels, and put the tubes and the absorbent material in a ziplock bag. Seal the bag and place it inside a bubble bag and tape the whole thing together tightly. Attach your shipping label to the outside of the bubble bag.
SECTION: Collecting Hydra
8. Store your Hydra somewhere cool where the ambient temperature won't get above 75°F (24°C) until you are ready to ship. Do not put them in the refrigerator.