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[DESCRIPTION]
<p>The UPPA-PROTEIN Concentrate™ kit is used to quantitatively concentrate dilute protein samples as low as 1ng/ml into a small volume. Protein precipitation and concentration are not affected by the presence of detergents, chaotropics, or other common laboratory agents.</p>
<p> </p>

[BEFORE_START]
<p><em>Chill OrgoSol Buffer at –20°C for ~1hr or more before use- see step 10 of the protocol.</em></p>
<p> </p>
<p><em><strong>NOTE:</strong></em> Perform the entire procedure in the cold (ice bucket) unless specified otherwise. Concentration should be performed in a centrifuge tube. For small volumes, use microfuge tubes. Always position microfuge-tubes in the centrifuge at the same orientation, i.e. cap-hinge facing out-ward. This will allow the pellet to remain glued to the same side of the tube during repeated centrifugations and minimize the loss of protein pellets.</p>

[GUIDELINES]
<p><strong>INTRODUCTION</strong></p>
<p>PROTEIN-Concentrate™ kit uses a proprietary reagent, Universal Protein Precipitation Agent (UPPA; Patent Pending). Protein solutions as dilute as 1ng/ml can be quantitatively concentrated into a small volume. Protein precipitation is not affected by the presence of detergents, chaotropics, or other common laboratory agents. After precipitation, the precipitate is washed to remove salts and other agents which produces protein samples of conductivity ~ 40-60µS - ideal for critical analysis. The protein precipitate is suspended in a small volume buffer. If the protocol is followed correctly, the recovery is generally 100%.</p>
<p> </p>
<p>The PROTEIN-Concentrate™ kit is supplied in two sizes: the Micro Kit is for concentrating up to a total of 10 ml dilute protein solution, either single or multiple procedures, and the Medi Kit is for concentration up to a total of 30 ml dilute protein solution, either single or multiple procedures. Additional volumes of any reagent may be purchased separately.</p>
<p> </p>
<p><strong>APPLICATIONS</strong></p>
<p>Suitable for concentrating proteins for running gels, raising antibodies, protein purification, protein assays, and other applications. This kit is not suitable for those proteins which may lose some of their biological activities when precipitated, for such proteins use either Column-PROTEIN Concentration™ kit (Cat. #786-126) or OrgoSol PROTEIN Concentration™ kit (Cat. # 786-125).</p>
<p> </p>
<p><strong>ITEM(S) SUPPLIED</strong></p>
<table style="width: 284px;">
<tbody>
<tr style="height: 40px;">
<td style="width: 10px; height: 40px;"><strong>Cat. #</strong></td>
<td style="width: 55px; height: 40px;"><strong>786-120 (Micro)</strong></td>
<td style="width: 69px; height: 40px;"><strong>786-121 (Medi)</strong></td>
</tr>
<tr style="height: 20px;">
<td style="width: 10px; height: 20px;">UPPA- I</td>
<td style="width: 55px; height: 20px;">30ml</td>
<td style="width: 69px; height: 20px;">100ml</td>
</tr>
<tr style="height: 20px;">
<td style="width: 10px; height: 20px;">UPPA- II</td>
<td style="width: 55px; height: 20px;">30ml</td>
<td style="width: 69px; height: 20px;">100ml</td>
</tr>
<tr style="height: 20px;">
<td style="width: 10px; height: 20px;">OrgoSol Buffer</td>
<td style="width: 55px; height: 20px;">50ml</td>
<td style="width: 69px; height: 20px;">2 x 50ml</td>
</tr>
<tr style="height: 20px;">
<td style="width: 10px; height: 20px;">UPC-Wash</td>
<td style="width: 55px; height: 20px;">2.0ml</td>
<td style="width: 69px; height: 20px;">2 x 2.0ml</td>
</tr>
<tr style="height: 20px;">
<td style="width: 10px; height: 20px;">SEED</td>
<td style="width: 55px; height: 20px;">300µl</td>
<td style="width: 69px; height: 20px;">2 x 300µl</td>
</tr>
<tr style="height: 40px;">
<td style="width: 10px; height: 40px;">Solubilization Buffer-I</td>
<td style="width: 55px; height: 40px;">2.0ml</td>
<td style="width: 69px; height: 40px;">2 x 2.0ml</td>
</tr>
<tr style="height: 40px;">
<td style="width: 10px; height: 40px;">Solubilization Buffer-II</td>
<td style="width: 55px; height: 40px;">0.5ml</td>
<td style="width: 69px; height: 40px;">2 x 0.5ml </td>
</tr>
</tbody>
</table>
<p> </p>
<p><strong>STORAGE CONDITIONS</strong></p>
<p>The kit is shipped at ambient temperature. Store all the components at room temperature upon arrival.</p>
<p><em>NOTE: Chill OrgoSol Buffer at –20°C for ~1hr or more before use- see step 10 of the protocol.</em></p>
<p> </p>
<p><strong>ADDITIONAL ITEMS REQUIRED</strong></p>
<p>Centrifuge, Centrifuge Tubes, Microfuge, &amp; Spin Columns</p>
<p> </p>
<p><em><strong>NOTE:</strong> </em>Perform the entire procedure in the cold (ice bucket) unless specified otherwise. Concentration should be performed in a centrifuge tube. For small volumes, use microfuge tubes. Always position microfuge-tubes in the centrifuge at the same orientation, i.e. cap-hinge facing out-ward. This will allow the pellet to remain glued to the same side of the tube during repeated centrifugations and minimize the loss of protein pellets.</p>
<p> </p>
<p><strong>PROCESSING LARGE SAMPLES </strong></p>
<p>Samples containing &gt; 100µg protein produces large and tightly packed protein pellets which require a longer time to dissolve in Buffers. Grinding of the protein pellet with a pestle will accelerate solubilization of the pellet. We recommend use of microfuge tubes and tight fitting pestle for processing samples containing larger than 100µg protein. See related products for ordering information.</p>

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