eamag/protocols-with-steps huggingface dataset:
TITLE: Schistosoma mansoni cercariae shedding
AUTHORS: Sarah K Buddenborg

[DESCRIPTION]
Schistosoma mansoni cercariae can be obtained from infected Biomphalaria glabrata snails. Although infected snails will release (shed) cercariae constantly at low numbers, it is possible to trigger the release of larger quantities by keeping the snails in the dark for a few days before shedding.

Material obtained from these procedures can be mechanically transformed into schistosomula for further experimentation.


[STEPS]
SECTION: Preparing snails for shedding
3.  Place snails into labelled 12- or 24-well plates with one snail per well

SECTION: Shedding snails
4. Place cover on plate and place snails under LED or fluorescent lighting for up to 2 hours to trigger release of cercariae from the infected snails. 
 

SECTION: Shedding snails
6. Check snails for shedding by viewing each well under a zoom stereomicroscope with diascopic illumination stand
SECTION: Collecting cercariae
9. Return shedding snails to a new tank with food and appropriate labels – make sure their air stones are under the water level and bubbling properly (See protocol "Snail husbandry for maintaining the Schistosoma mansoni life cycle")
 

SECTION: Preparing snails for shedding
1. Place aquarium tank containing snails >=4 weeks post-exposure to S. mansoni on a stable bench top 
 
 
SECTION: Preparing snails for shedding
2. Using featherweight forceps, remove snails from the tank and rinse gently with 1x aquarium water in a wash bottle

This is critical in order eliminate false positives (snails with cercariae on them from a different shedding snail)
SECTION: Collecting cercariae
8. Dispose of snails that did not shed by putting them in a container klorsept (See "Guidelines and Warnings") or place in new tank if you'd like to re-shed after an additional week. 
SECTION: Collecting cercariae
7. Collect water containing cercariae every 30 minutes (alternatively, you can just collect cercariae at the end of 2 hrs shedding; this will give you the most concentrated cercariae suspension) using a transfer pipet and top up wells with new 1x aquarium water

If needed (i.e. for monomiracidium-infected snails where separate sexes are needed), keep cercariae from each snail separate and use new transfer pipet for each snail
SECTION: Shedding snails
5. Add enough 1x aquarium water to each well so that they are covered
TITLE: Production of AAV Virus by Iodixanol Gradient Ultracentrifugation
AUTHORS: Allen Institute for Brain Science

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is used to produce AAV of any serotype with high purity and high titer.</div><div class = "text-block"><span style = "font-weight:bold;">Note</span><span>: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.</span></div></div>

[STEPS]
TITLE: Grip Strength
AUTHORS: Xiaobo Mao, Ramhari Kumbhar, Hanseok Ko, Valina L. Dawson, Ted Dawson

[DESCRIPTION]
Use to measure rodent grip strength

[STEPS]
1. Set up grip strength meter on table.
2. Hold the mouse gently by the tail, and place either its forepaws or all paws on the grip strength grid.
3. Gently pull the mouse back until it releases its grasp.
4. Record the maximum holding force displayed on the grip strength meter.
TITLE: Purification of Plasmid DNA by Miniprep
AUTHORS: Georgina Elisa Diego Macías, Ana Belem García González, Irán Alessandra Chaparro Rodríguez, Jair Alexis Gardea Sáenz

[DESCRIPTION]
Extraction of Plasmid DNA by Miniprep PureLink T M Quick Plasmid Miniprepa Kits

[STEPS]
1. Centrifuge 1-5 mL of the overnight LB-culture. Remove all medium.

2. Add 250 µL Resuspension Buffer (R3) with RNase A to the cell pellet and resuspend the pellet until it is homogeneous

3. Add 250 µL Lysis Buffer (L7). Mix gently by inverting the capped tube until the mixture is homogeneous. Do not vortex. Incubate the tube at room temperature for 5 min.

4. Add 350 µL Precipitation Buffer (N4). Mix immediately by inverting the tube, or for large pellets, vigorously shaking the tube, until the mixture is homogeneous. Do not vortex. Centrifuge the lysate at >12,000 × g for 10 min

5. Load the supernatant from step 4 onto a spin column in a 2-mL wash tube. Centrifuge the column at 12,000 × g for1 min. Discard the flowthrough and place the column back into the wash tube.

6.  Add 500 µL Wash Buffer (W10) with ethanol to the column. Incubate the column for 1 min at room temperature. Centrifuge the column at 12,000 × g for 1 min. Discard the flowthrough and place column back into the wash tube 

7. Add 700 µL Wash Buffer (W9) with ethanol to the column. Centrifuge the column at 12,000 × g for 1 min. Discard the flowthrough and place the column into the wash tube. Centrifuge the column at 12,000 × g for 1 min. Discard the wash tube with the flowthrough

8. Place the Spin Column in a clean 1.5-mL elution tube. Add 75 µL of preheated TE Buffer (TE) to the center of the column. Incubate the column for 1 minat room temperature.

9. Centrifuge the column at 12,000 × g for 2 minutes. The elution tube contains the purified plasmid DNA. Discard the column. Store plasmid DNA at 4°C (short term) or store the DNA in aliquots at −20°C (long term).
TITLE: Protein-A and Protein-LG Sandwich ELISA
AUTHORS: Angel  Justiz-Vaillant

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This ELISA was used to study the interactions between Staphylococcal protein-A (SpA) and protein-LG (SpLG) with different immunoglobulin preparations from mammalian and avian species.</div></div>

[STEPS]
?. This ELISA was used to study the interactions between Staphylococcal protein-A (SpA) and protein-LG (SpLG) with different immunoglobulin preparations from mammalian and avian species. The 96 well microtiter plate was coated overnight at 4°C with 2 µg/µl per well of SpA in carbonate-bicarbonate buffer pH 9.6.
?. The plate was then treated with bovine serum albumin solution and washed 4X with PBS-Tween. 50 µl of immunoglobulins (1 mg/ml) is added and incubated for 1h at room temperature, and the microplate is rewashed 4X with PBS-Tween.
?. Then, 50 µL of peroxidase-labeled SpLG conjugate diluted 1:5000 in PBS-non-fat milk was added to each well and incubated for 1h at RT. The plate was washed 4X with PBS-Tween. Then, 50 µL of o-phenylenediamine solution (4 mg/mL) was added, and the plate was incubated for 15 min at RT in the dark.
?. The reaction was stopped with 50 µL of a 3M H2SO4 solution. The plate was visually assessed for color development and read on a microplate reader at 492 nm. A cut-off point was calculated as the mean of the optical density of the negative controls multiplied by two. The cut-off point was set to 0.32.
TITLE: PCR algorithm to detect and characterize Neisseria meningitidis carriage isolates in the African meningitis belt
AUTHORS: Olivier Manigart

[DESCRIPTION]
<h3>Improved methods for the detection and characterization of carried Neisseria meningitidis isolates are needed.  We evaluated a multiplex PCR algorithm for the detection of a variety of carriage strains in the meningitis belt. To further improve the sensitivity and specificity of the existing PCR assays, primers for gel-based PCR assays (sodC, H, Z) and primers/probe for real-time quantitative PCR (qPCR) assays (porA, cnl, sodC, H, E, Z) were modified or created using Primer Express software. Optimized multiplex PCR assays were tested on 247 well-characterised carriage isolates from six countries of the African meningitis belt. The PCR algorithm developed enabled the detection of N. meningitidis species using gel-based and real-time multiplex PCR targeting porA, sodC, cnl and characterization of capsule genes through sequential multiplex PCR assays for genogroups (A, W, X, then B, C, Y and finally H, E and Z). Targeting both porA and sodC genes together allowed the detection of meningococci with a sensitivity of 96% and 89% and a specificity of 78% and 67%, for qPCR and gel-based PCR respectively. The sensitivity and specificity ranges for capsular genogrouping of N. meningitidis are 67% - 100% and 98%-100% respectively for gel-based PCR and 90%-100% and 99%-100% for qPCR. We developed a PCR algorithm that allows simple, rapid and systematic detection and characterisation of most major and minor N. meningitidis capsular groups, including uncommon capsular groups (H, E, Z).</h3>

[STEPS]
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TITLE: Biochemical Measures of Neuropathy - Glutathione Reductase
AUTHORS: Eva Feldman

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:</span></div><div class = "text-block">Oxidative stress is highly correlated with the metabolic changes caused by hyperglycemia. Increased levels of glucose overload mitochondria and result in the production of reactive oxygen species (ROS). In addition, the flow of excess glucose through cellular pathways decreases the cell’s normal ability to detoxify ROS. As a result, the neurons and axons of the peripheral nervous system contain increased levels of ROS and decreased antioxidant capacity. The following assays are used to measure these changes in rodent models of diabetic neuropathy. </div><div class = "text-block"><span style = "font-weight:bold;">Diabetic Complication:</span></div><div style = "text-align :; float : ;"><img style = "" src = "https://s3.amazonaws.com/pr-journal/bixb4kcn.jpg" /></div></div>

[STEPS]
?. [Sample Preparation — Tissue:]
1. Homogenize the tissue in 5–10 mL of cold assay buffer (i.e., 50 mM potassium phosphate, pH 7.5, 1 mM EDTA) per gram of tissue. (General Equation: μL Buffer = mg Tissue X 10)
2. Centrifuge at 10,000 x g for 15 minutes at 4°C.
3. Remove supernatant for assay and store on ice. Sample can be stored at –80°C for at least one month.
?. [Performing Assay:]
1. Turn on Multiskan and open file GRx.sed.2. Background Wells: add 120 μL of Assay Buffer and 20 μL of GSSG to three wells.3. Positive Control Wells: add 100 μL of Assay Buffer, 2 0μL of GSSG, and 20 μL of diluted GR (control) to three wells.4. Sample Wells: add 100 μL of Assay Buffer, 20μL of GSSG, and 2 0μL of sample to three wells.5. Initiate reactions by adding 50 μL of NADPH to all wells as quickly as possible. Note precise time the reaction is initiated.6. Place plate onto Multiskan holder and click START. 7. Save raw data as an Excel file into the GRx data folder. Use the naming convention gxXXXX.xls, where XXXX is the date in mmdd format.8. Select Process>Organize. Choose the appropriate data to organize (usually Measure1), then click OK. This rearranges the data into columns.9. Save organized data as an Excel file into the GRx data folder. Use the naming convention grXXXXXX.xls, where XXXXXX is the date in mmddyy format.
TITLE: Probe qPCR assay for Charybdis japonicum 
AUTHORS: Jackson Treece, Gert-Jan Jeunen, Jo-Ann Stanton, Neil Gemmell

[DESCRIPTION]
Charybdis japonica or the Asian Paddle Crab is a non-native crab species to Aotearoa, New Zealand. It was first detected in 2000 and has since spread along the eastern Northland coast where it is a threat to local native species. This test was developed to aid in detecting the invasive crustacean's presence using environmental DNA sampling. 
The probe based qPCR assay targets a 208bp portion of the COX-1 gene with an indicative limit of detection of 1.0E-4 ng/µl genomic DNA input or 92 molecules/µl with a synthetic standard (IDT gBlock). Species specificity was successfully challenged against a panel of 14 New Zealand native crab species. Further challenge with local species is recommended. 

[STEPS]
SECTION: Master Mix
2. 

10.0 µl SensiFAST Probe 2x Mix 
0.8 µl 10µM Forward primer 
0.8 µl 10µM Reverse primer 
0.2 µl 10µM FAM Probe 
6.2 µl PCR Water
2.0 µl Template
20µl total reaction volume

SECTION: Thermal Conditions
3. 95°C - 3 minutes
95°C - 15 seconds
60°C - 30 seconds   x45 cycles

QuantStudio 3 (ThermoFisher Scientific) used during assay development.
SECTION: Control Sequence
4. Sythesized positive control sequence (IDT gBlocks)

tattactgccattcttctactcctctcgcttcctgtgttagctggagctattactatattattaacagaccgaaacttaaatacttcattcttcgaccctgctgggggtggggatcctgttctttatcaacacttgttttgattctttggacatcctgaagtttatattttaatcttacctgccttcggaataatttcacatattgtcagacaagaatctggtaaaaaggaatcatttggtactttgggtataatttatgctataatggctattggcattttaggctttattgtatgagctcatcacatgtttacagttggaatagatgtagatactcgagcatattttacatcagctactataattattgctgtgcctactggaattaaaatttttagatgacttagaactcttcacggcactcaaattaactatagaccttctatattatgggccctaggatttattttcttattcacagtaggaggtcttacaggagtagttttagcaaactcttccattgatattattcttcacgacacatactatgtggtagcccactttcattacgttctttctataggagctgtatttggaattttcgccggcattgcccactgattccccttatttaccggactttcattaaatcctaaatgaataaaaattcattttactattatatttattggtgtaaatattactttctttcctcaacactttttaggactaaatggcatgcctcgtcgttattctgactaccccgatgcatacactacgtgaaatattgtctcttctataggatcaatagtatcacttattgctatactaatttttataattgtaatttgagaagcacttatctcaaatcgacctgttctattttctcctttccttccttcttctattgaatgaaaccactcttacccacctgcagatcactcatacatagaaatccccctaatctctaacttctaaaatggcagatagatgtatagg
SECTION: Materials
1. Meridian Bioscience SensiFAST Probe Lo-ROX Mix (2x)
PCR quality water
Template DNA
Oligonucleotides (10uM concentration):
Forward primer: Cj_1295F	ATGGCATGCCTCGTCGTTAT
Reverse primer: Cj_1498R	CTGCAGGTGGGTAAGAGTGG
FAM Probe:        Cj_1323P	CCCCGATGCATACACTACGT
SECTION: Species for Assay Development and Specificity Testing
5. Many thanks to the National Institute of Water and Atmospheric Research (NIWA), the Marine Invasives Taxonomic Service (MITS) and Deakin University for contributions from their invertebrate tissue collection. Donation ID: 2023-032 D
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
This is a very simple protocol&nbsp;showing how to extract haemolymph from adult Drosophila melanogaster. (Based on protocols from Sigma Aldrich).

[STEPS]
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TITLE: SOP52v1_TGD_IIDP-HIGISampleProcessing
AUTHORS: Varsha Rajesh

[DESCRIPTION]
This workflow details the processing of acinar tissue obtained as part of the HIGI cohort (IIDP), by TGD Lab. 

[STEPS]
SECTION: Recieving Samples
1. When acinar samples are received from the various isolation centers, match what is physically sent with sample info list sent with package and make sure all samples are accounted for. Take note of the condition of the samples. 
SECTION: Recieving Samples
2. Let isolation center know samples were received and in what condition. 
SECTION: Recieving Samples
3. Print QR barcodes for the acinar samples in this format and tape onto the tubes. 

HIGI#ACIN (# being the next sequential number)
SECTION: Recieving Samples
4. Store tubes in Yalow -80°C freezer in  "IIDP - HIGI Acinar Samples" box.
SECTION: Extracting DNA
5. Thaw acinar tubes on ice. 
SECTION: Extracting DNA
6. Use DNA extraction protocol for DNeasy Blood & Tissue Kit (https://protocols.io/view/dna-extraction-qiagen-dneasy-blood-amp-tissue-kit-bjngkmbw). 
SECTION: QC
7. After extraction, measure the concentration by nanodrop, taking account of 260/280 and 260/230 values as well. 260/280 value should be close to 1.8 and 260/230 should be around 1.8-2.0. 
SECTION: QC
8. Take a portion of the sample to measure concentration by Qubit. Protocol can be found here: https://protocols.io/view/qubit-dsdna-assays-bnstmeen. For genotyping, Qubit concentration should be above 50 ng/uL and total DNA should be above 750 ng. 
SECTION: Genotyping
10. Record sample info, nanodrop QC and qubit information in genotyping QC spreadsheet, which will be sent to the SFGF core whenever the entire batch is ready (follow this protocol: https://www.protocols.io/edit/sop54v1-tgd-genotypingsubmissionforcollaborators-b8jaruie).
SECTION: QC
9. Create QR codes that include sample name in this format: HIGI#DNA. Label should also include nanodrop concentration and QC info. 
SECTION: Genotyping
11. As of 2023-12-01, samples were sent to be genotyped on Illumina's Infinium Omni Exome 2.5 arrays.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
NYCIII  (en-why-see-three) culture media<br />Suitable for the culture of Gardnerella vaginalis

[BEFORE_START]
Heat inacticate your serum. Serum can kill many different types of bacteria.

[STEPS]
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TITLE: No Title
AUTHORS: 

[DESCRIPTION]
This protocol provides a method for analysis of the relative abundance of potential antibiotic resistance genes found in the virome. We use the Comprehensive Antibiotic Resistance Database (CARD) as our reference for potential antibiotic resistance genes.

[GUIDELINES]
Required Software:<br />
<ul>
<li>CARD</li>
<li>VFDB</li>
<li>NCBI's BLAST+ v2.2.0</li>
<li>Bowtie2-2.1.0</li>
</ul>
Relevant Files<br />Output:<br />
<ul>
<li>CARD_abx_resistance/CARD_annotated_orfs_in_otu_table.tsv</li>
<li>CARD_abx_resistance/orf_otu_table.tsv</li>
</ul>
Perl Script: calculate_abundance_from_sam.pl<br />R Script: <a href="https://www.protocols.io/view/Script-R14-CARD-Antibiotic-Resistance-Analysis-ejnbcme" target="_blank">R14</a>

[STEPS]
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TITLE: CHEM 584--Cloning sgRNA Sequences into lentiCRISPRv2
AUTHORS: Ken   Christensen

[DESCRIPTION]
This protocol describes how to clone your chosen sgRNA sequence into the lentiCRISPRv2 plasmid. A method to validate your cloning is also included here.

[STEPS]
SECTION: Design and Order Guide Sequences
1. Design and order sgRNA sequence oligonucleotides based on your previously identified target sequences.

Oligo 1: 5'-CACCGNNNNNNNNNNNNNNNNNNNN
Oligo 2: 3'-          CNNNNNNNNNNNNNNNNNNNNCAAA

Important Note: Do not include the NGG PAM in your designed oligonucleotides
SECTION: lentiCRISPRv2 Digestion
2. Digest 2 µg of lentiCRISPRv2 plasmid with BsmBI overnight at 55°C:

Set up the following reaction:
    2.5 µl 10X NEB r3.1 Buffer
    x µl ddH2O to make up 25 µl final volume
    1 µl BsmBI
    2 µg lentiCRISPRv2 plasmid*

    *Add the plasmid last, into the reaction mixture, pipette gently to mix and spin briefly.
SECTION: Gel Purify the Digested Plasmid
3. Use the Zymoclean Gel DNA Recovery kit to gel purify the digested plasmid.

A ~2 kb filler piece should be present on the gel. Only purify the larger band. Leave the ~2 kb band.
SECTION: Anneal the oligonucleotides
4. Suspend the oligonucleotides at 100 µM in autoclaved ddH2O
SECTION: Anneal the oligonucleotides
5. Set up annealing reaction:
    
    2 µl Oligo 1
    2 µL Oligo 2
    2 µl 10X T4 Ligation Buffer (NEB)
    14 µl ddH2O
SECTION: Anneal the oligonucleotides
6. Anneal the oligonucleotides in a thermocycler:

    95°C for 5 min
    Ramp temperature down to 25°C at 5°C/min
SECTION: Anneal the oligonucleotides
7. Dilute annealed oligonucleotides 1:200 with autoclaved ddH2O (1 µl annealed oligo pair + 199 µl water).
SECTION: Ligation into lentiCRISPRv2
8. Set up the ligation reaction and incubate at 16°C overnight (with the 2X Instant Stick End Master Mix, you may be able to significantly shorten this incubation).

    x µl digested plasmid (50 ng)
    1 µl diluted oligonucleotide duplex
    6 µl 2X Instant Sticky End Master Mix
    x µl ddH2O to make a final volume of 12 µl
SECTION: Transform into NEB STBL competent cells
9. Add up to 5 µl of your ligation reaction into NEB STBL Mix & Go component cells following the Mix & Go Competent Cell protocol. Plate your cells on Amp/Carb plates.
SECTION: Use Colony PCR to screen for the presence of cloned oligo pairs
10. Use the Colony PCR protocol to screen for the presence of cloned oligo pairs using the 2X TaqDog Master Mix.

Run 10-20 µl of your PCR products on a 0.8% agarose gel. Use the 100 bp ladder for your gel.

Primers (provided):

    lentiCRISPRv2 For: GTGGAAAGGACGAAACACCG
    lentiCRISPRv2 Rev: CTAGGCACCGGATCAATTGC

Expected amplicon for positive clones = 248 bp
SECTION: Isolate plasmid for positive clones
11. Prepare overnight cultures for positive clones.

Purify plasmid from overnight cultures using the Zymo Plasmid Miniprep-Classic protocol.
SECTION: Sequence positive plasmids
12. Send plasmid for Sanger Sequencing (Eton Biotechnology).

Sequencing primer:
    hU6-F: GAGGGCCTATTTCCCATGATT
TITLE: EXPRESSION AND PURIFICATION OF HUMAN p62 (HIS-TEV-mCherry-p62)
AUTHORS: Gabriele Zaffagnini, Elisabeth Holzer

[DESCRIPTION]
This protocol describes how to express and purify human p62 tagged N-terminally with HIS and TEV. The expression is performed with E. coli Rosetta pLysS cells. The protein is purified via a HisTrap column and gel filtration (SEC). 

[BEFORE_START]
Lysis Buffer:
25 mM HEPES pH 7.5
150 mM NaCl
25 mM Imidazole
2 mM MgCl2
Freshly added: 2 mM β-Mercaptoethanol, Roche Protease Inhibitor (Merck, #5056489001), DNAse I (Sigma, #DN25-1G)

HisTrap Buffer A (filtered, degassed):
25 mM HEPES pH 7.5
500 mM NaCl
25 mM Imidazole
Freshly added: 2 mM β-Mercaptoethanol

HisTrap Buffer B (filtered, degassed):
25 mM HEPES pH 7.5
500 mM NaCl
400 mM Imidazole
Freshly added: 2 mM β-Mercaptoethanol

SEC Buffer (filtered, degassed):
25 mM HEPES pH 7.5
500 mM NaCl
Freshly added: 1 mM DTT

[STEPS]
SECTION: Expression of p62
1. Grow E. coli Rosetta pLysS cells in 8 L Luria broth medium at 37 °C until an OD600 nm of 0.8 is reached.

SECTION: Expression of p62
2. Induce protein expression with 150 micromolar (µM) IPTG for 300 min at 25 °C.

SECTION: Expression of p62
3. Centrifuge cells at 3000 rcf, 4 °C, 15 min

SECTION: Expression of p62
4. Aspirate media and resuspend pellet in 10 mL Lysis Buffer

SECTION: Expression of p62
5. Flash freeze sample in liquid nitrogen
SECTION: Expression of p62
6. Store at -80 °C until use.

SECTION: Purification of p62
7. Thaw sample at 37 °C

SECTION: Purification of p62
8. Sonicate sample 5 cycles at 65% power for 30 s

SECTION: Purification of p62
9. Centrifuge sample 140,000 rcf at 4 °C for 30 min

SECTION: Purification of p62
8.1. Repeat sonication for a total of three times
SECTION: Purification of p62
10. Equilibrate a HisTrap HP column (GE Healthcare) with 5 column volumes of water and 5 of HisTrap Buffer A
SECTION: Purification of p62
9.1. During centrifugation perform equilibration
SECTION: Purification of p62
11. Filter the supernatant through   syringe filter 

SECTION: Purification of p62
12. Load sample onto the equilibrated HisTrap column at 4 °C

SECTION: Purification of p62
13. Elute protein via a stepwise imidazole gradient using HisTrap Buffers A and B and elution steps: 25 mM, 62.5 mM, 118.75 mM, 148.75 mM, 212.mM, 306.25 mM and 400 mM imidazole
SECTION: Purification of p62
14. Add  100 µg  to 150 µg TEV protease to pooled protein-containing fractions and incubate 30 s at 4 °C to cleave.

SECTION: Purification of p62
15. Concentrate resulting protein with a 30 kDa MWCO concentrator (Millipore) to between .5 mL and 1 mL 

SECTION: Purification of p62
16. Apply protein to a Superdex 200 Increase 10/300 column (Cytiva) pre-equilibrated with SEC Buffer.
SECTION: Purification of p62
17. Freeze purified protein in liquid nitrogen and store at -80 °C

SECTION: Purification of p62
18. Protein purity can be determined by SDS PAGE analysis
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Haematoxylin Eosin (H&amp;E) staining of mouse intestinal tissue</p>

[STEPS]
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TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>The IL-8 ELISA protocol here described was established by Stephan Odenbreit and modified in this form by Luisa F. Jiménez-Soto.</p>
<p>Here are the publications using this protocol:</p>
<p>DOI:<a href="https://doi.org/10.1371/journal.pone.0035341" target="_blank">10.1371/journal.pone.0035341</a></p>
<p>DOI:<a href="https://doi.org/10.1128/IAI.00364-09" target="_blank">10.1128/IAI.00364-09</a></p>
<p>DOI:<a href="https://doi.org/10.1078/1438-4221-00205" target="_blank">10.1078/1438-4221-00205</a></p>
<p>and  PMID:11886563</p>

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