eamag/protocols-with-steps huggingface dataset:
TITLE: Immunohistochemistry for anti-GFP
AUTHORS: Lauren C. Faget, Thomas Hnasko

[DESCRIPTION]
Hnasko lab protocol for anti-GFP immunohistochemistry

[STEPS]
SECTION: Protocol:
1. Day 1    
SECTION: Protocol:
1.1. Wash with 1X PBS (Phosphate Buffered Saline, 10X solution, Fisher bioreagents, BP399-1) pH 7.4 5 min 

SECTION: Protocol:
1.2. Wash with 1X PBS containing  0.2% Triton X-100 (Sigma-Aldrich, X100-1L) (PBS-T). 5 min 

SECTION: Protocol:
1.3. Blocking step with appropriate normal serum (use serum from the source species for the secondary antibodies to prevent nonspecific binding). 
Incubate with 4% NDS (Normal Donkey Serum, Jackson ImmunoResearch, 017-000-121) in PBS-T. 60 min at Room temperature

SECTION: Protocol:
1.4. Incubate with primary antibodies in PBS-T with 4% NDS.
5 min at 4 °C
Wrap well-plate air-tight with parafilm and cover with foil.
Rabbit anti-GFP polyclonal antibody, Invitrogen, A11122 (*Upon arrival: Diluted 1:1 in glycerol by adding same volume 100% glycerol, and stored at -20C). Dilution: 1:1000 to have a final dilution of 1:2000. Example: 1 µL anti-GFP + 999 µLPBS-T 4% NDS  

SECTION: Protocol:
2. Day 2
SECTION: Protocol:
2.1. Wash with 1X PBS-T. 10 min

SECTION: Protocol:
2.2. Incubate with secondary antibodies in 1X PBS-T.
120 min at Room temperature
Cover well-plate with foil. 
AlexaFluor 488 Donkey anti-rabbit, Jackson ImmunoResearch, 711-545-152 (*Upon arrival: Reconstituted with ~0.4 ml 50% glycerol and stored at -20C). Dilution: 1:400. 
Example: 1 µL D@Rb488 + 399 µL PBS-T

SECTION: Protocol:
2.3. Wash with 1X PBS. 10 min

SECTION: Protocol:
2.4. Mount on Slides (22-034-979, Fisherbrand). Takes practice!
SECTION: Protocol:
2.5. Let dry on a slide holder (VWR, 48429-105). Cover from light
SECTION: Protocol:
2.6. Rinse with ddH2O. Briefly dip slide in a 50mL falcon tube filled with ddH2O
SECTION: Protocol:
2.7. Place back on slide holder and let dry covered from light
SECTION: Protocol:
2.8. Apply ~120 µLof Fluoromount-G Fluorescent mounting medium (SouthernBiotech, 0100-01) with 0.5 μg/ml DAPI (Millipore, 10236276001) on dried slides and apply coverslip (2980-225, Corning)

SECTION: Protocol:
2.9. Protect from light and dry overnight before imaging
SECTION: Purpose:
6. GFP or YFP amplification. This protocol can also be applied to other epitopes/antibodies.
SECTION: Material:
3. Use a 24-well plate. Place a maximum of 12 sections / well. Use 1 mL/ well for washes and blocking. Use from 500 µL to 750 µL / well for antibody incubations. Use fire-shaped blunted Pasteur glass pipette or thin brush to transfer section from 1 well to another. Place well plate on shaker, rocker, or belly dancer for all washes and incubation steps.
SECTION: Note:
4. Discard any sections found stuck to side of wells

SECTION: Samples:
5. 30um-sections in 48 well-plates at 4 °Cin 1X PBS with 0.01% Sodium Azide (Fisher Scientific,
BP922I-500) solution
TITLE: Zona pellucida removal using  Acid Tyrode solution and snap freezing
AUTHORS: Elizabeth Jannaman, Peter J. Hansen

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A simple protocol to remove the zona pellucida from bovine embryos. This is a necessary step before freezing embryos for analysis of RNA or DNA because of contaminating cumulus and sperm cells associaed with the zona pellucida. </div></div>

[STEPS]
?.
TITLE: Mask Building Idea
AUTHORS: Erica Wells

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Our limited findings</span><span>: This “DIY folding mask” tries to replicate some of the benefits of a n95 mask. It has been saccharin tested by several nurses and an NP. I cannot say these are as effective as a factory n95 mask because of course a real one would be better. But I tested it against a CDC recommended bandana and scarf and it seems to out perform them. It also seems to outperform the surgical mask.</span></div></div>

[STEPS]
?. Make your pattern. Cut out your first set and use them for tracing. No need for perfection. The felt is around 9 x 12 inches. It's not wildly important that the measurements are exact. It's the shapes really. As long as it fits your face.
?. Glue bottom to front. Match up curved edges.
?. Glue top to front so that it can be tucked under the bottom. Glue along edges.
?. Cut 6 1/2 inches of beading wire. Use pliers to curl ends.
?. Glue wire into bottom part of mask. Glue to edge.
?. Fold over
?. Apply glue to wire curls liberally. Allow time to dry.
?. Cut 2 1/2 inches of flat wire and glue to front.
This will be used to adjust over the nose.
?. Cut about 12 to 14 inches of elastic x 2
?. Glue elastic to mask.
?. Tuck inner front into mask. No need for perfection.
?. Fit snuggly to face. Use wires to form. Apply like n95. YouTube “n95 mask application” if needed
TITLE: Poly-d lysine coating slides
AUTHORS: Grace Burgin

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">coating standard blank slides with poly-d lysine to increase adhesion of tissue </div></div>

[STEPS]
?. Make .3mg/ml in 0.1M borate buffer [ → add 16.67 ml 0.1M borate to 5mg bottle of poly-D lysine lyophylized powder]
?. coat the slides once by dipping
?. Dry
?. Rinse twice with dH20
?. Dry
TITLE: Bacterial Media Preparation
AUTHORS: Andrew Crowley

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for the preparation of bacterial culture and recovery media, with options for several types of antibiotics.</div><div class = "text-block">Default scale - 1 L</div></div>

[STEPS]
?. Add powdered LB ( ) to deionized water ( )
25 g
1 L
n.b.Particulates may remain after addition, but these will dissolve during the autoclave cycle
?. Autoclave using liquid cycle
?. Allow media to cool to
50 °C
?. Select antibiotic from the list below:
?. Add  kanamycin and mix thoroughly
50 mg
?. Add  ampicillin and mix thoroughly
100 mg
?. Select the type of media from the list below:
?. Add powdered tryptic soy broth ( ) to deionized water ( )
30 g
1 L
n.b.Particulates may remain after addition, but these will dissolve during the autoclave cycle
?. Autoclave using liquid cycle
TITLE: Cleanbox UV-C sterilization chamber
AUTHORS: Ryan  Straight

[DESCRIPTION]
Using the Cleanbox CX2 UV-C sterilization chamber.

[STEPS]
1. Ensure the Cleanbox is powered on. (Green light in front will be lit.)


2. Insert the item to be sterilized into the Cleanbox. For headsets, hang using the headstrap from the platform inside the chamber, so the lens portion is hanging down. If the equipment you're cleaning is wired, run the wires through the small hole at the bottom of the front of the chamber.
3. Close the Cleanbox door completely. This Cleanbox will not run unless both doors are completely shut.
4. Press the silver button at the top center of the front of the chamber. The formerly green light will turn red, the fan inside the Cleanbox will turn on, and the UV light will begin sterilizing.
5. When the light turns green and the fan stops, you are free to remove the equipment. Be sure to shut the door.
TITLE: Quantitative analysis method of mRNA expression using dual RNA in situ hybridization by comparison with housekeeping genes
AUTHORS: Byung Joon Seung

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">In retrospective formalin-fixed paraffin-embedded (FFPE) samples, RNA quality of FFPE tissues showed variation following the storage period and fixation process. So we suggest quantitative analysis by dual detection of target mRNA and housekeeping genes in one section and calculated the target gene/housekeeping gene ratio by two open-source image analysis programs. </div><div class = "text-block">This assay potentially allows for reliable quantification of mRNA expression levels in retrospective FFPE samples. </div></div>

[STEPS]
?. [RNA-ISH original image]
RNA-ISH images were acquired at ×400 magnification.
?. [Counting the blue dots]
The original images were converted using Fiji program [1].[1] Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, et al. Fiji: an open-source platform for biological-image analysis. Nat Methods. 2012;9(7):676-82.
?. [Counting the blue dots]
Original images were converted using "Image→Color→Dichromacy→Tritanope" by Fiji programSave as Tiff file.
?. [Counting the red dots]
Tritanope-filtered images were converted to a CIELAB (RGB to CIELAB) and ‘a’ channel images were acquired as jpg files using Fiji program.
?. [Counting the red dots]
Select regions of interest (ROI) in 'a' channel images.To measure red dots, white spot detection mode in the spot detector of ICY program was used.Size of spots to detect (scale 3). Find adequate filter conditions for your research.
?. [Counting the blue dots]
Select regions of interest (ROI) in Tritanope filtered images.To measure blue dots, dark spot detection mode in the spot detector of ICY program [2] was used. Size of spots to detect (scale 2).Find adequate filter conditions for your research.[2] De Chaumont F, Dallongeville S, Chenouard N, Hervé N, Pop S, Provoost T, et al. Icy: an open bioimage informatics platform for extended reproducible research. Nat Methods. 2012;9(7):690-6.
TITLE: Crystallisation protocol for SARS-CoV-2 nsp3 macrodomain in P1 21 1
AUTHORS: Jasmin Aschenbrenner, Peter Marples, Lizbé Koekemoer, Charlie Tomlinson, Daren Fearon

[DESCRIPTION]
The COVID-19 pandemic has demonstrated the need for novel therapeutic interventions and improved pandemic preparedness strategies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This protocol details an optimized crystallization method for the SARS-CoV-2 nsp3 macrodomain, a potential drug target. Using sitting drop vapor diffusion with seeding, we describe specific buffer conditions and procedures to consistently produce high-quality crystals suitable for XChem fragment screening. The method yields crystals that diffract to an average resolution of 1.5 Å, enabling high-resolution structural studies and can also be used for compound development through co-crystallization experiments. 
All structures solved during the development of tool compounds for the SARS-CoV-2 nsp3 macrodomain are deposited on the PDB (Group deposition: G_1002283).

[STEPS]
SECTION: Crystallization experiment
4. Protein and buffer requirements:
115.2 µL21.6 mg/mL  
2.88 mL 
28.8 µL   seeds, dilution 1:2

SECTION: Crystallization experiment
6. Dispense 30 µL into SwissCI 3 lens plate reservoir wells using a 100 µl multi-channel pipette.
Dispense 400 nL21.6 mg/mL    to each lens using the SPT mosquito.
Dispense  300 nL to each lens using the SPT mosquito.
Dispense  100 nL to each lens using the SPT mosquito.

Drop ratio: 4:3:1 ratio (400 nl   : 300 nl reservoir solution: 100 nl seeds)
Final drop volume: 800 nl
SECTION: Crystallization experiment
5. Crystallisation screen composition:
0.1 Molarity (M)  MES pH 6.5 
30% w/v PEG 3000

Stock solutions used:
1 Molarity (M)  MES adjusted to pH 6.5 with HCl
50% w/v PEG 3000

 

SECTION: Crystallization experiment
8. Crystal form after ~12 h.
 


SECTION: Crystallization experiment
7. Incubate at 20 °C for 1440 min in Formulatrix Rock Imager.

Imaging Schedule: The first images are taken after 12 h and the imaging schedule follows a Fibonacci sequence of days for further collections. 
SECTION: Equipment needed
2. Formulatrix Rock Imager (or incubator of choice)
SPT mosquito
 
P100 8 multi-channel pipette
SwissCI 3 lens plate

SECTION: Crystallization experiment
3. Prepare seed stock:
 
1: 2 dilution    seeds
SECTION: Data Collection at Synchrotron
9. Diamond Light Source
Unattended Data Collection (UDC)
Data Collection Temperature: 100K
Detector: DECTRIS EIGER2 X 9M
Beamline: I04-1
Wavelength:  0.9212 Å
Resolution (Å): 1.62
Beam Size (μm): 60 X 50
Number of images: 3600
Oscillation: 0.10°
Exposure (s): 0.0020
Transmission (%): 100
Flux (ph/s): 3.80e+12
SECTION: SARS-CoV-2 nsp3 macrodomain expression and purification
1. The protein used for crystallisation was expressed and purified using the following protocol.
TITLE: Polymer-brush-bilayers-under-stationary-shear-motion-at-linear-response-regime
AUTHORS: Mike Edwards

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Statistical mechanics is employed to tackle the problem of polymer brush  bilayers under stationary shear motion. The article addresses, solely,  the linear response regime in which the polymer brush bilayers behave  very much similar to the Newtonian fluids. My approach to this  long-standing problem split drastically from the work already published </span><span style = "font-style:italic;">Kreer, T</span><span>,  Soft Matter, 12, 3479 (2016). It has been thought for many decades that  the interpenetration between the brushes is source of the friction  between the brush covered surfaces sliding over each other. Whiles, the  present article strongly rejects the idea of interpenetration length in  that issue. Instead, here, I show that structure of the whole system is  significant in friction between brush covered surfaces and the  interpenetration is absolutely insignificant. The results of this  research would blow one’s mind about how the polymer brush bilayers  respond at small shear rates.</span></div></div>

[STEPS]
TITLE: No Title
AUTHORS: 

[GUIDELINES]
<strong>YTSS</strong> <br />&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;per 500 ml<br />
<table>
<tbody>
<tr>
<td>&nbsp;</td>
<td><strong>1/2</strong></td>
<td><strong>1/10</strong></td>
</tr>
<tr>
<td>Trypton</td>
<td>1.25 g</td>
<td>0.25 g</td>
</tr>
<tr>
<td>Yeast Extract</td>
<td>2 g</td>
<td>0.4 g</td>
</tr>
<tr>
<td>Sea Salts</td>
<td>10 g</td>
<td>10 g</td>
</tr>
<tr>
<td>Agar</td>
<td>7.5-9 g</td>
<td>7.5-9 g</td>
</tr>
</tbody>
</table>
<br />For TM1040, use 1/2 YTSS, 30C, in dark, 200-250 rpm if in liquid;<br />For Jannaschia CCS1 and CCS2, use 1/10 YTSS, 22-24C, in dark, 200-250 rpm if in liquid. <br />For all other Roseos, use 1/2 YTSS.<br /><br />Note from Matt: The 1/2x (30&deg;C+shaking) is for the bugs that are more coastal, and the 1/10thx (22-24&deg;C + shaking) is for the bugs that are more open ocean.<br /><br />Sea Salts: use 100 kDa permeate from TFF seawater to prepare media and this will take care of &ldquo;sea salts&rdquo; for the medium.

[STEPS]
TITLE: Personality disorder in an Early Intervention Psychosis cohort: Findings from the Social Epidemiology of Psychoses in East Anglia (SEPEA) study
AUTHORS: Ka-young Ban, D Osborn, Y Hameed, S Pandey, J Perez, PB Jones, JB Kirkbride

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Aim: Personality Disorders (PD) often share clinical and phenomenological overlap with psychotic disorders, especially at onset. However, there is little research on comorbid PD among people experiencing first episode psychosis. We examined the prevalence of PD recording and its sociodemographic and clinical correlates in people accepted to Early Intervention in Psychosis (EIP) services. </div><div class = "text-block">Methods: Participants were aged 16-35, accepted into 6 EIP services for suspected psychosis, as part of the Social Epidemiology of Psychoses in East Anglia (SEPEA) study. PD was recorded by clinicians according to ICD-10. Multilevel logistic regression was performed. </div><div class = "text-block">Results: Of 798 participants, 76 people (9.5%) received a clinical diagnosis of PD, with emotionally unstable PD (75.0%, N=57) the most common subtype. In multivariable analysis, risk factors for PD included female sex (odds ratio [OR]: 3.4; 95% CI: 2.0-5.7), absence of psychotic disorder after acceptance to EIP (OR: 3.0; 95% CI: 1.6-5.5), more severe hallucinations (OR: 1.6; 95% CI: 1.2-2.1), and lower parental SES (OR: 1.4; 95% CI: 1.1-1.8). Compared with the white British, black and minority ethnic groups were less likely to receive a PD diagnosis (OR: 0.3; 95% CI: 0.1-0.7). There was no association between PD and neighbourhood-level deprivation or population-density. </div><div class = "text-block">Conclusions: Recording of a PD diagnosis was three times more common amongst participants later found not to meet threshold criteria for psychotic disorder, implying phenomenological overlap at referral which highlights difficulties encountered in accurate diagnostic assessment, treatment and onward referral. People with PD experienced more individual-level, but not neighbourhood-level social disadvantage in an already disadvantaged sample.</div></div>

[STEPS]
TITLE: Lung Homogenization
AUTHORS: Timothy S C Hinks, Bonnie van Wilgenburg, Huimeng Wang, Liyen Loh, Marios Koutsakos, Katherine Kedzierska, Alexandra J. Corbett, Zhenjun Chen

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is part 3.4 of the "Study of MAIT Cell Activation in Viral Infections In Vivo" collection of protocols.</div><br/><div class = "text-block"><span style = "font-weight:bold;">Collection Abstract: </span><span>MAIT cells are abundant, highly evolutionarily conserved innate-like lymphocytes expressing a semi-invariant T cell receptor (TCR), which recognizes microbially derived small intermediate molecules from the riboflavin biosynthetic pathway. However, in addition to their TCR-mediated functions they can also be activated in a TCR-independent manner via cytokines including IL-12, -15, -18, and type I interferon. Emerging data suggest that they are expanded and activated by a range of viral infections, and significantly that they can contribute to a protective anti-viral response. Here we describe methods used to investigate these anti-viral functions in vivo in murine models. To overcome the technical challenge that MAIT cells are rare in specific pathogen-free laboratory mice, we describe how pulmonary MAIT cells can be expanded using intranasal bacterial infection or a combination of synthetic MAIT cell antigen and TLR agonists. We also describe protocols for adoptive transfer of MAIT cells, methods for lung homogenization for plaque assays, and surface and intracellular cytokine staining to determine MAIT cell activation.</span></div></div>

[STEPS]
?. Collect the lungs into .
[RPMI supplemented with penicillin/streptomycin]
?. For homogenization, place the lung and the   into 10 mL falcon tubes with lids (see Note 16).
[media]
?. Prepare 10 or 15 mL Falcon tubes with 2 ×  for cleaning the homogenization probe initially and 1 tube containing HBSS. For each group of samples, prepare further 1 ×  and 1 × , and for the final probe clean set up 2 × .
[80%w/v EtOH]
[EtOH]
[HBSS]
[EtOH]
?. Homogenize the sample using a homogenizer, mounted on a retort stand with the probe set to medium for  per sample. Keep samples  (see Note 17).
on ice
?. Centrifuge the samples at .
Centrifuge: 1000 34, 7 min
?. Using a 1 mL pipette, carefully draw up approximately (a little bit more is good), avoiding the pellet and fatty residue on top. Divide this volume into two 1.5 mL Eppendorf tubes. Store at  for subsequent plaque assays.
[supernatant]
-80 °C
TITLE: Staining of Dissociated Lung Cells for Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE sequencing)
AUTHORS: Gautam Bandyopadhyay, Jeffrey Malik, Heidie Huyck, Ravi Misra, John_Ashton, Gloria S Pryhuber

[DESCRIPTION]
This article describes step by step protocol for antibody-staining frozen lung cells for CITE sequencing.

[STEPS]
SECTION: Thawing of frozen dissociated human lung cells
1. Frozen cells were thawed in 37°C water bath and soon after the freezing medium melted cells were transferred into a 15 ml conical tube and the tubes were filled with staining buffer [1% BSA (w/v, Millipore) in DPBS (Biowhittaker)] to dilute the DMSO in freezing medium.
SECTION: Thawing of frozen dissociated human lung cells
2. Cells were centrifuged at 800g, 4°C for 10 minutes.
SECTION: Thawing of frozen dissociated human lung cells
3. After centrifugation supernatants were discarded and cells were resuspended in 1.0 ml staining buffer.
SECTION: Fc receptor blocking and  antibody staining
4. Viable cell counts were determined by trypan blue exclusion assay
SECTION: Fc receptor blocking and  antibody staining
5. Cells were then incubated in staining buffer containing 2% v/v normal mouse serum (Millipore Sigma) for 10 minutes over ice for Fc-receptor blocking.
SECTION: Fc receptor blocking and  antibody staining
6. Cells were then washed with staining buffer and incubated at a final concentration of 1 × 106 cells per 100 μl of staining cocktail containing analyte-specific antibodies (attached table) for 30 min at 4°C.  

SECTION: Fc receptor blocking and  antibody staining
7. After antibody-staining, cells were washed three times (800g, 4°C for 10 minutes) and resuspended in 100 μl staining buffer and passed through a 100-μm strainer (Falcon, Corning) and sent Genomic Research Center, University of Rochester Medical Center for further processing for CITE sequencing.
TITLE: SCN Cyst Extraction from Greenhouse Cultures (crocks)
AUTHORS: Casey A Schlenker

[DESCRIPTION]
SCN Cyst Extraction from Greenhouse Cultures (crocks)

[STEPS]
SECTION: Preparing Green House Cultures for Cyst Extraction
1. Put on gloves to protect hands before beginning preparation
SECTION: Preparing Green House Cultures for Cyst Extraction
2. Using gardening shears, clip off all of the stems on the chosen soybean plant, leaving behind 1-2 inches of the stem. Place stems in the 3-gallon bucket.
SECTION: Preparing Green House Cultures for Cyst Extraction
3. Place crocks on cart for transport back to the lab. Dispose of the soybean clippings in either the steam cart or the compost.
SECTION: SCN cyst extraction
4. *Once you begin this process for a crock you must finish the whole crock within the work day. 
SECTION: SCN cyst extraction
5. Lay out small petri dishes on a tray and set aside.
SECTION: SCN cyst extraction
6. To begin collecting cysts from greenhouse cultures, flood the crock gently with water. Utilize the faucet with long rubber tubing.
SECTION: SCN cyst extraction
7. A white cutting board with a hole is placed on-top of a white tub. On top of the cutting board hole place the 20/60 mesh sieves.
SECTION: SCN cyst extraction
8. Fill a grey bucket with water and place close to crock.
SECTION: SCN cyst extraction
9. Once properly soaked, remove the long skinny tube from the center of the crock, and place aside.
SECTION: SCN cyst extraction
10. Choose your first sample. Remove the tag from the sample and set aside. Pull the stem and the tube out of the crock at the same time, being careful not to rip the stem.
SECTION: SCN cyst extraction
11. Move the root and tube to the gray wash bucket and gently swirl to dislodge any sand and turface from the tube.
SECTION: SCN cyst extraction
12. Place the washed root on top of the 20/60 sieves and blast the roots, with the handheld sprayer, turning the root over periodically to ensure all cysts are properly dislodged. A good indicator of when all the cysts are removed is when all the turface is dislodged from the roots.
SECTION: SCN cyst extraction
13. After checking that the root is thoroughly clear of cysts, dispose of the root in a 3-gallon bucket and rinse 20 sieve briefly.
SECTION: SCN cyst extraction
14. Using a wash bottle, wash all material from 60 sieve into a small petri dish. Replace the petri dish lid and place the tag from the corresponding sample on top of the petri dish.
SECTION: SCN cyst extraction
15. Repeat steps 6-10 with the remaining tubes.
SECTION: Cleaning the crocks
16. To clean the crocks, you must thoroughly wash all growing substrate and plant matter out of the crock. Scrub the crocks to ensure all previous plant material is removed.
SECTION: Cleaning the crocks
17. Place all the pvc tubing into the crock (22 in total, 1 long tube) and fill 80% with water. Fill the rest of the crock with bleach. Let sit for a minimum of 24 hours.
SECTION: Cleaning the crocks
18. Drain and let dry.