TITLE: BG11 medium (working group Wilde)
AUTHORS: Christin Köbler, Annegret Wilde

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">100x BG11 (500 ml)</span></div><div class = "text-block"><span>74.79 g NaNO</span><span style = "vertical-align:sub;">3</span></div><div class = "text-block"><span>3.75 g MgSO</span><span style = "vertical-align:sub;">4</span><span> × 7H</span><span style = "vertical-align:sub;">2</span><span>O</span></div><div class = "text-block"><span>1.8 g CaCl</span><span style = "vertical-align:sub;">2</span><span> × 2H</span><span style = "vertical-align:sub;">2</span><span>O</span></div><div class = "text-block">0.30 g Citric Acid</div><div class = "text-block"><span>0.28 ml 0,5 M Na</span><span style = "vertical-align:sub;">2</span><span>EDTA (pH 8)</span></div><div class = "text-block"><span>Add </span><span style = "font-style:italic;">Aqua bidest</span><span> (</span><span style = "font-weight:bold;">ddH</span><span style = "font-weight:bold;vertical-align:sub;">2</span><span style = "font-weight:bold;vertical-align:sub;">O</span><span>) to a final volume of 500 ml. Aliquot (5x100 ml) and autoclave. Store at 4°C.</span></div><div class = "text-block"><span style = "font-weight:bold;">Trace-Metal-Mix (500 ml)</span></div><div class = "text-block"><span>1.43 g H</span><span style = "vertical-align:sub;">3</span><span>BO</span><span style = "vertical-align:sub;">3</span></div><div class = "text-block"><span>900 mg MnCl</span><span style = "vertical-align:sub;">2</span><span> × 4H</span><span style = "vertical-align:sub;">2</span><span>O</span></div><div class = "text-block"><span>110 mg ZnSO</span><span style = "vertical-align:sub;">4</span><span> × 7H</span><span style = "vertical-align:sub;">2</span><span>O</span></div><div class = "text-block"><span>195 mg Na</span><span style = "vertical-align:sub;">2</span><span>MoO</span><span style = "vertical-align:sub;">4</span><span> × 2H</span><span style = "vertical-align:sub;">2</span><span>O</span></div><div class = "text-block"><span>39.5 mg CuSO</span><span style = "vertical-align:sub;">4</span><span> × 5H</span><span style = "vertical-align:sub;">2</span><span>O</span></div><div class = "text-block"><span>24.7 mg Co(NO</span><span style = "vertical-align:sub;">3</span><span style = "vertical-align:sub;vertical-align:sub;">)</span><span style = "vertical-align:sub;">2</span><span> × 6H</span><span style = "vertical-align:sub;">2</span><span>O</span></div><div class = "text-block"><span>Put some</span><span style = "font-weight:bold;"> ddH</span><span style = "font-weight:bold;vertical-align:sub;">2</span><span style = "font-weight:bold;vertical-align:sub;">O</span><span> into the bottle; dissovle the salts seperately and fill up to 500 ml. Sterile filter and aliquot the solution  (10 x 50 ml). Store at 4°C.</span></div><div class = "text-block"><span style = "font-weight:bold;">2x BG11 (1 L)</span></div><div class = "text-block">20 ml 100x BG11</div><div class = "text-block"><span>2 ml K</span><span style = "vertical-align:sub;">2</span><span>HPO</span><span style = "vertical-align:sub;">4</span><span> × 3H</span><span style = "vertical-align:sub;">2</span><span>O (30 mg/ml)</span></div><div class = "text-block"><span>2 ml Na</span><span style = "vertical-align:sub;">2</span><span>CO</span><span style = "vertical-align:sub;">3</span><span> (20 mg/ml)</span></div><div class = "text-block">2 ml Trace-Metal-Mix</div><div class = "text-block">Add 900 ml deionized water, afterwards add the solutions, fill up to 1 L with deionized water and autoclave. Store at RT.</div><div class = "text-block">Before use add 2 ml of Fe-Ammonium-Citrate (6 mg/ml). </div><div class = "text-block"><span style = "font-weight:bold;">1xBG11 (1 L)</span></div><div class = "text-block">10 ml 100xBG11</div><div class = "text-block">10 ml TES pH 8</div><div class = "text-block"><span>1 ml K</span><span style = "vertical-align:sub;">2</span><span>HPO</span><span style = "vertical-align:sub;">4</span><span> (30 mg/ml)</span></div><div class = "text-block"><span>1 ml Na</span><span style = "vertical-align:sub;">2</span><span>CO</span><span style = "vertical-align:sub;">3</span><span> (20 mg/ml)</span></div><div class = "text-block">1 ml Trace-Metal-Mix </div><div class = "text-block">Add 900 ml deionized water, afterwards add the solutions, fill up to 1 L with deionized water and autoclave. Store at RT.</div><div class = "text-block">Before use add 1 ml of Fe-Ammonium-Citrate (6 mg/ml). </div></div>

[STEPS]