eamag/protocols-with-steps huggingface dataset:
TITLE: Aureococcus anophagefferens population count, and relative size (Violet SSC) by flow cytometry (CytoFLEX S Flow Cytometer Beckman Coulter)
AUTHORS: Emily E. Chase, Alex Truchon, Steven W Wilhelm

[DESCRIPTION]
A method for obtaining relative cell size, population density, etc., of the brown tide algae Aureococcous anophagefferens by a Violet Side Scatter configuration on a Beckman Coulter CytoFLEX S Flow Cytometry System (CytExpert software). 


[BEFORE_START]
Boot up the CytExpert Software and follow the start up protocol. Familiarity with the machine and its setting will permit necessary adjustments for your experiments and help ensure accurate results. 

[GUIDELINES]
To achieve the intended results of this protocol a configuration using a violet laser must be setup for a CytoFLEX S Flow Cytometer. This protocol is based on a CytoFLEX with Violet SSC on the Violet laser at the 405/10 position. See the "Setting Up Violet Side Scatter (VSSC) Channel" section of the CytoFLEX manual. While visualization of A. anophagefferens can be achieved without a violet laser, it will not be possible to determine relative cell size. 

[STEPS]
SECTION: CytoFLEX Experiment Setup
1. Create a new experiment with sample acquisition settings as follows: 
FSC 200
SSC 40
VioSSC 75
FITC 200
PerCP 150
PB450 1 [placeholder; this could be set for a different dye than Pacific Blue shown here, this will not change the results]
Threshold: (manual) PerCP 5000

Set the flow rate to medium (30 μL/minute), the sampling to 60 seconds (not by events), and the display to 100,000 events. 


 


  

SECTION: Sampling Process
3. 250 μL of each sample to be measured (e.g., A. anophagefferens culture) are pipetted into a 96 well CoStar [REF#3795] Assay Plate round bottom plate (can be substituted) and loaded into the CytoFLEX. After opening the "Plate" window and clicking "Add Plate", samples can then be labelled. 

 

SECTION: CytoFLEX Experiment Setup
2. Optional Create a combination of density plots and histograms best suited for your analyses. An example set up is provided in the image below (Figure 2), where A. anophagefferens populations are clearly achieved. It is recommended to set up a histogram with time on the x-axis (final histogram) to account for the machine's measuring consistency as sampling will often start out slower and then stabilize. This can be applied to any gated population you have selected by clicking the text at the top of the histogram and selecting the population within the dropdown menu. 

   

SECTION: Sampling Process
4. Labelled samples can then be run sequentially using the "Auto Record". Upon completion data can be either exported (most conveniently as a .csv) or reviewed using the "Statistics" window according to all events and events within user defined gated populations. Population counts are achieved by number of events within the A. anophagefferens gates, and subsequent relative size of events (cells) can be achieved through Violet Side Scatter channel results.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<div>
<p>Laboratory protocols for preparation of blood smears, reporting of malaria parasites, processing of blood samples using malaria rdt (hrp-2), blood grouping and design of questionnaire, employed in this study are briefly described.</p>
</div>
<div> </div>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Modified beetle rearing medium
AUTHORS: 孟筠 陳

[DESCRIPTION]
Modified beetle rearing medium

[STEPS]
SECTION: Start making the medium!
1. 
First, Add the dry ingredients

 



 


SECTION: Start making the medium!
2. Second, add the wet ingredients

 

SECTION: Start making the medium!
3. MIx all of the ingredients in the box and fill into the tube for 3/4 full (about 75ml)

2. 
2. 
2. 
SECTION: Start making the medium!
5. Cut the tin foil into small pieces and fold it twice to cover the open side of the tube.
SECTION: Start making the medium!
6. Autoclave the filled tube at 120°C for 20 min.
SECTION: Start making the medium!
4. Use the stick to compress the ingredients inside the tube.
SECTION: Start making the medium!
7. Done!
TITLE: Transformation using electroporation 
AUTHORS: Ashwinuday 

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">For transforming cells with the DNA of interest using electroporation as the tool.</div></div>

[STEPS]
?. To an electroporation cuvette add electro-competent cells of the required strain,  of DNA of interest and  of autoclaved ultrapure water (milliQ)
50 µl
70 ng
50 µl
?. Keep the cuvette in ice for
?. Pulse the cuvette using a pulser using the bacteria setting at 2.5keV
Ensure that there is no sparking
?. To the cuvette add 2X LB up to same volume
?. Incubate the cuvette at for
37 °C
?. Spread the contents of the cuvette onto an LA plate with appropriate antibiotics
?. Incubate the plate overnight at
37 °C
TITLE: RNA TRIzol isolation of Datura spp.
AUTHORS: Eunice Kariñho-Betancourt, rtapia

[DESCRIPTION]
We present the TRIzol protocol for RNA extraction. We employed leaf tissue from vegetative and reproductive plants from four solanaceous species of the genus Datura; D. stramonium, D. pruinosa, D. inoxia and D. wrightii.

[BEFORE_START]
Make sure all your lab devices and supplies are sterilized

[GUIDELINES]
Set flash-frozen tissue

Prepare reagents

Isolate and store RNA

Quantify and verify RNA quality and integrity

[STEPS]
SECTION: REAGENTS
1. Reagents

Chloroform:Isoamyl alcohol (24:1) 100ml
    96 ml Chloroform
    4 ml Isoamyl alcohol

Saline solution 500 ml 
    0.8M Sodium Citrate
    1.2 M NaCl
    Distilled water

75% ethanol 100 ml
    75 ml of ethanol molecular biology grade (100%)
    25 ml of Distilled water

Isopropyl alcohol (Isopropanol) molecular biology grade

TRIzol reagent (Invitrogen/Thermofisher)

DNAsa I

RNase free water

SECTION: RNA ISOLATION
2. Protocol
Grind tissue to a powder in liquid nitrogen with cold (-80°C 1 hr sterilized) mortar and pestle

SECTION: RNA ISOLATION
3. In a 1.5 ml Eppendorf tube add 50-100 mg of ground tissue plus 1ml of TRIzol Reagent and 5 μl of DNAsa I. Mix by vortex 30 seconds. 
SECTION: RNA ISOLATION
4. Add two sterilized steel beads and high-speed shake the tube in a Tissuelyser II (QIAGEN), at 30 Hz for 45 seconds. Repeat 2 more times
SECTION: RNA ISOLATION
5. Take out the beads and incubate the mixture at room temperature in the same Eppendorf tube for 5 min.
SECTION: RNA ISOLATION
6. Centrifuge the tube at 13,000 rpm for 15 min at 4°C. All of the insoluble matter should form a pellet at the bottom of the tube.
SECTION: RNA ISOLATION
7. Transfer the aqueous phase (upper) in a new 1.5 ml tube and add 250 μl of isoamyl alcohol. Mix by inversion.
SECTION: RNA ISOLATION
8. Centrifuge the tube at 13,000 rpm for 15 min at 4°C.
SECTION: RNA ISOLATION
9. Transfer the upper aqueous phase in a new 1.5 ml tube and add 250 μl of Saline solution and then 250 μl of isopropanol.
SECTION: RNA ISOLATION
10. Mix by inversion and incubate on ice for 10 min.
SECTION: RNA ISOLATION
11. Centrifuge at 13,000 rpm for 10 min at 4°C, and decant the supernatant, taking care not to lose the pellet.
SECTION: RNA ISOLATION
12. Add 1 ml 75% cooled ethanol to the pellet and centrifuge at 13,000 rpm for 10 min at 4°C and decant the supernatant
SECTION: RNA ISOLATION
13. Open the cap and air-dry the pellet.
SECTION: RNA ISOLATION
14. Add 30μl RNase-free water and heat at 55°C to resuspend the pellet carefully.
SECTION: RNA ISOLATION
15. Analyze (quantify concentration, quality, and integrity of the RNA) and store the tube at -80°C.
SECTION: RNA QUALITY AND INTEGRITY
16. The integrity of each sample of RNA was first visualized by electrophoresis in 1.0% agarose stained with Ethidium Bromide (EtBr). The gel was run in TBE 0.5X buffer prepared in water treated with DEPC.

Then the quantity and quality of RNA samples were verified by UV absorbance in Nanodrop (Thermo Scientific) and by fluorescence measurements with Qubit 3.0 (Invitrogen) using 2ul of each sample of RNA .
TITLE: KAPP-Sen TMC: Pancreas Donor Acceptance Criteria
AUTHORS: Cristina Aguayo-Mazzucato, Kanako Iwasaki

[DESCRIPTION]
Pancreas Donor Acceptance Criteria

[STEPS]
SECTION: Inclusion Criteria
1. Both sexes
Age > 18 years
All ethnicities
SECTION: Exclusion Criteria
2. HTN with end-organ damage
BMI > 35
GFR < 60 ml/min/1.73 m2
Known cardiovascular disease
Pancreatitis
Active malignancy
Chronic pulmonary, hepatic, infectious, gastrointestinal, endocrine, neurologic, or inflammatory disease
Chronic use of systemic steroids 
Use of agents with senolytic activity
TITLE: Th1 Polarization of Mouse CD4+ Cells
AUTHORS: Sam Li

[DESCRIPTION]
<div class = "text-blocks"></div>

[STEPS]
?. [Isolation of CD4+ Cells From Lymph Nodes]
Harvest lymph nodes (superficial cervical, mandibular, axillary, inguinal, and mesenteric) from mice.
?. [Isolation of CD4+ Cells From Lymph Nodes]
Tease lymph nodes through a sterile 70-µm nylon cell strainer to obtain single-cell suspensions incomplete RPMI containing 10% FCS (complete medium).
?. [Isolation of CD4+ Cells From Lymph Nodes]
Resuspend cells in complete medium and use your favorite method to isolate CD4+cells. Consider using our Mojosort™ Mouse CD4 T Cell Isolation Kit.
?. [Th1 Polarization of CD4+ Cells]
On day 0, coat 12-well plate with anti-mouse CD3ε, clone 145-2C11 (3µg/ml). Incubate at 37°C for 2 hours or 4°C overnight. Aseptically decant antibody solution from the plate. Wash plate 3 times with sterile PBS. Discard liquid.
?. [Th1 Polarization of CD4+ Cells]
Plate CD4+ cells at 1.0 x 106 /1ml/well. Culture cells for 5 days at 37°C, 5% CO2, in the presence of anti-mouse CD28, clone 37.51 (3 µg/mL), anti-mouse IL-4, clone 11B11 (10 µg/mL), recombinant mouse IL-2 (5 ng/mL), and recombinant mouse IL-12 (10 ng/ml).
?. [Th1 Polarization of CD4+ Cells]
On day 3, if media is yellow, add 2 ml/well of fresh media.
?. [Th1 Polarization of CD4+ Cells]
After harvesting, the cells are ready for staining.
?. [Th1 Polarization of CD4+ Cells]
On day 5, wash cells once and then restimulate in complete media with 50 ng/ml PMA, 1 μg/ml ionomycin and 10 µl monensin (1000x), in a 6-well plate in incubator at 37°C for 5 hours.
TITLE: Pyruvate and Urea preparation for dissolution DNP
AUTHORS: Dave Korenchan, Hikari Yoshihara, Sukumar Subramaniam, Romelyn DeLos Santos, Renuka   Sriram, John Kurhanewicz

[DESCRIPTION]
This document codifies the probe preparation of pyruvate and urea for dissolution dynamic nuclear polarization. Additionally, this also describes the steps recommended for estimation of T1 and polarization and quality assurance to ensure repeatability and robustness of hyperpolarized 13C probe formulation. 

[STEPS]
TITLE: Universal Immunoblot analysis for investigating Protein-AG (SpAG)-binding to avian and mammalian  immunoglobulins.
AUTHORS: Angel  Justiz-Vaillant

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A protein that combines the binding capacity of SpA and SpG is not comercially available. It can be easy created in the laboratory by combining these two immunoglobulin-binding proteins to horseradish peroxidase by the periodate method [1]. However, a mixture of SpA and SpG could have the same effect as universal reagent in immunodetection. </div><div class = "text-block">1. Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175 </div></div>

[STEPS]
?. Aliquots of egg yolks, animal sera or 2 µg/µl of purified immunoglobulins from birds, laboratory, wild, farm animals and pets are applied to the gels of SDS-PAGE as described elsewhere.
?. Gels are transferred to nitrocellulose membranes (Immobilon-Nc, pore size 0.45 µm, Sigma-Aldrich Co, St Louis, Missouri) during 71 minutes at 40 mAmps using a semi-dry electroblotter, HEP-1 Model, Owl Scientific Inc.
?. The running buffer contains 25 mM Tris, 192 mM glycine pH 8.3 and 20% methanol.
?. The nitrocellulose membranes are blocked overnight in 10% non-fat skim milk in PBS with 0.05% Tween-20 pH 7.4 and then washed 4x, 10 minutes with PBS-Tween 20.
?. A mixture of SpA and SpG at a concentration of 5 µg/ml is added to the membranes. A recombinant Protein-AG (SpAG) could be used instead.
?. After that there is an incubation period of 12 hours at 4°C. It may be an overnight incubation period.
?. The nitrocellulose membranes were washed as above.
?. A secondary antibody (rabbit anti-chicken IgY horseradish peroxidase, Sigma Aldrich) is added at a 1:15 000 dilution.
?. It is incubated for one hour at room temperature and washed as above.
?. Tetramethyl-benzidine (TMB) solution is added to the nitrocellulose membranes, which are then incubated in the dark for seven minutes.  Then, the membranes are shaken gently and rinsed thoroughly in de-ionized water to stop the blotting process and are left to dry.
?. Alternatively, Ig samples are transferred to nitrocellulose membranes and directly probed using SpAG-HRP (diluted 1:5000) and then adding TMB (this system was mainly used for detecting avian Igs).
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This protocol describes an ELISA assay  for measurement of FAS ligand (FASL, CD95L) in supernatants collected from T cell dependent cytotoxicity (TDCC) cultures after BiTE® treatment.</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Be the Banksy-Monat love child you've always wanted to be.</p>

[GUIDELINES]
<p>Materials needed: sharpened pencil, eraser, at least a 4 inch by 4 inch piece of unlined paper</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>RNA extraction of plant tissues (in our case Bauhinia leaves) using a pBIOZOL/CTAB lysis buffer</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: P14 PROCEDIMIENTO DE RECLAMACIONES
AUTHORS: cgarcia

[DESCRIPTION]
Las reclamaciones y sugerencias serán presentadas por los doctorandos, directores y/o tutores o agentes externos que tengan relación con la EIDUCAM en la Secretaría Técnica de la EIDUCAM (eiducam@ucam.edu).

Para realizar una reclamación/sugerencia existe un formulario que se encuentra a disposición de los grupos de interés en el Portal del Doctorando

http://investigacion.ucam.edu/doctorado/portal-del-doctorando/sugerencias .

[STEPS]
SECTION: P14 PROCEDIMIENTO DE RECLAMACIONES
1. Rellenar el formulario presente en la página web de la EIDUCAM (enlace).
SECTION: P14 PROCEDIMIENTO DE RECLAMACIONES
2. La Secretaría Técnica de la EIDUCAM recepciona, registra y estudia las reclamaciones y sugerencias que le sean presentadas y, posteriormente las trasmite al Secretario de la Comisión Académica del Programa de doctorado correspondiente.
SECTION: P14 PROCEDIMIENTO DE RECLAMACIONES
3. La Comisión Académica del Programa de doctorado correspondiente estudiará la reclamación o sugerencia y contestará al solicitante en un plazo no superior a 15 días. También hará la comunicación a la Secretaría Técnica de la EIDUCAM.
SECTION: P14 PROCEDIMIENTO DE RECLAMACIONES
4. La Secretaría Técnica de la EIDUCAM archivará las reclamaciones una vez resueltas.
TITLE: COVIDscanDX mScanner Protocol (Confidential)
AUTHORS: don.cooper 

[STEPS]
?. [COVIDscanDX mScanner Instructions for Use]
For iOS iPad and iPhone devices newer than 2018 use the following procedure.  To scan the result of the developed test open the Safari browser on the iPad or iPhone device and navigate to www.register.covidscandx.com.For Android Samsung Galaxy Tab A 10.1 inch (2019) use the following procedure. To scan the result of the developed test open the default browser on the  tablet and navigate to www.register.covidscandx.com.
?. [COVIDscanDX mScanner Instructions for Use]
Enter the unique single-use product ID code included in the kit on the COVIDscanDX card. *Note the product code will expire after a valid image is automatically captured and a result is provided.  Keep the COVIDscanDX card and product code to access the detailed results in the future to share with your electronic record of your result with your health care provider, travel entry authority, employer etc.
?. [COVIDscanDX mScanner Instructions for Use]
Following valid product code entry a new page is displayed on the device that gathers information about testing location and conditions and/or test subject symptoms.  The user enters the information in the fields and provide a visual score of the test region signal ranging from 0-5 according to the COVIDscanDX card examples.  A score of 0 indicates no test signal is visible while a score of 5 indicates the strongest test signal.
?. [COVIDscanDX mScanner Instructions for Use]
The appliction will ask for permission to open the device camera application.  The user gives permission and positions the displayed blue box landmarks over the aruco targets and QR code until the blue boxes turn green and test image capture occurs within 1 second.  If the lighting conditions are not optimal for image capture an error messaage will be shown that instructs the user to adjust the lighting or position of the test cartridge and image again.
?. [COVIDscanDX mScanner Instructions for Use]
Once the images have been captured the user is notified and server-side algorithms check the images for lighting conditions and position.  A Positive, Negative or Invalid result will appear on the device display within 5 seconds (assuming web connectivity).  Depending on the result the user is given instructions on further support to establish the diagnosis of COVID-19 via telemedicine portal.
?. [COVIDscanDX mScanner Instructions for Use]
Post test results are accessible for review and integration via JSON or CSV format to partner database by site approved users through the partner dashboard.
TITLE: Streaking bacteria on a Petri Plate from -70C frozen culture
AUTHORS: Emma Tollafield, Dibyendu Dutta, Wolfram Moebius

[DESCRIPTION]
This protocol describes how to obtain isolated single colonies of any bacterial strain by streak plating, from a -70C frozen stock culture.

[STEPS]
SECTION: Creating a streak plate
1. Label bottom of LB agar plate with name, date, E. coli strain, and any additional contents such as antibiotics. 

 

SECTION: Creating a streak plate
2. Working aseptically, use inoculation loop to take E. coli from the frozen stock by swirling loop on surface of stock until slushy. 
 

SECTION: Creating a streak plate
3. Streak loop across one side of the agar plate. Discard loop (if using disposable plastic loop). 
SECTION: Creating a streak plate
4. Rotate the plate 90°, and starting at the end of the last set of streaks, use a fresh disposable loop (or sterilize the reusable loop) to create streaks on this side of the plate. Working clockwise, repeat with next two sides of the plate, using a fresh loop each time.
SECTION: Creating a streak plate
5. Incubate plate   at37 °C, upside down to prevent condensation.
TITLE: YT medium
AUTHORS: Andreas Sagen

[DESCRIPTION]
2xYT is a nutritionally rich liquid microbial growth medium used for propagation of recombinant strains of Escherichia coli and M13 bacteriophage or other filamentous single-stranded DNA bacteriophages.

[GUIDELINES]
Prepare enough for the necessary number of experiments to perform in order to prevent batch-to-batch variation between experiments. Input chemicals should be of a certain quality in order to prevent contaminants that can impact microbial growth and selection.

[STEPS]
SECTION: 500 mL 2x YT medium
1. Fill the bottle with 400 mL deionized water
SECTION: 500 mL 2x YT medium
2. Measure and add:
    8 g Tryptone
    5 g Yeast extract
    2.5 g Sodium chloride

Powdered compounds:
 
 
 
SECTION: 500 mL 2x YT medium
3. Adjust pH to pH 7.0 by adding drops of concentrated NaOH
SECTION: 500 mL 2x YT medium
4. Add deionized water to a total of 500 mL
SECTION: 500 mL 2x YT medium
5. Autoclave liquid at 121 °C for 15 min
TITLE: TaqMan qPCR INRAE eWHALE 
AUTHORS: Teddy Urvois, Anne-Laure Besnard, Erwan Quéméré

[DESCRIPTION]
Protocol for the TaqMan assay used by INRAE team in eWHALE ring test study

[BEFORE_START]
Lab work:
Put all equipment (micropipettes, disposable tips, microtubes, molecular grade water, qPCR plate) in UV cabinet and
light UV for 20 mins.
Thaw, vortex and spin samples.
Thaw and vortex Master Mix environnemental TaqMan‱ 2.0.

[STEPS]
SECTION: Master mix preparation
1. Determine the number of reactions, including negative controls and NTCs. Add 2-3 reactions to have margin for pipetting error.
Prepare the Master mix according following this volume for 1 reaction :
4 µL of Molecular Grade Water
10 µL Master Mix environnemental TaqMan‱ 2.0
1 µL Probe at 0.13 micromolar (µM)
1 µL Forward primer at 0.5 micromolar (µM)
1 µL Reverse primer at 0.5 micromolar (µM) 

Vortex and spin.
SECTION: Plate preparation
2. Add 17 µL of Master mix.

Add 3 µL of  .

Seal the plate with qPCR plate seal and centrifuge briefly (2 min at 2000g).
SECTION: qPCR
3. Put the plate in the thermal cycler. qPCR is performed with an initial denaturation at 95 °C for 10 min followed by 49 cycles of 30 s at 95 °C and 50 s at 60 °C.
TITLE: Lung COVID
AUTHORS: Solos Jaturapisanukul

[DESCRIPTION]

Primary
objectives:
-To study the Chest CT and
pulmonary function tests in ESRD patients after recovered from COVID-19

Secondary objectives

- To study the factors affecting the
pulmonary sequalae after COVID-19 in CKD patientssuch as oxygen requirement,ventilator need and levels of inflammatory
cytokines  such as interleukin-6 (IL-6)
and C-reactive protein (CRP)

Type of study: Prospective observational cohort study 
 
Inclusion Criteria:
      
CKD stage 5 requiring HD
or continuous peritoneal dialysis (CAPD ) for more than 3 months

       - Age 18-80 years
       - Diagnosis of COVID-19
confirmed by real time polymerase chain reaction (RT-PCR) and recovered for
more than3 months previously

Exclusion Criteria:

-   Patients with history of chronic lung
diseases i.e chronic obstructive pulmonary disease (COPD) and restrictive lung
disease

Criteria to withdraw from the protocol  Patients who have active disease after enrollment 
Participants  100 patients 

Place- Faculty of Medicine,Vajira Hospital ,Navamindradhiraj University



 
 




 
 


 
 
 
 
 
 
 



 


 


 



 
 
 
 
 
 
 
 

[GUIDELINES]


 


The study was conducted according to the Declaration of Helsinki and Good Clinical
Practice guidelines, and all study methods were carried out in accordance with
relevant guidelines and regulations. All patients participating in the study
signed an informed consent before enrollment.


14. Expected outcomes of the study


This trial contribute to new knowledge, including the
consequences of the most affected organ (lung) after COVID-19, which has rarely
been reported previously. In addition, the factors that associated with poor
pulmonary outcome will also be considered. 


15. Dissemination of results and publication policy


The
data supporting the findings of this study are available from the corresponding
author. 




[STEPS]
1. Follow-up Study of the Pulmonary Function and CT
scan finding in Chronic Kidney Disease Patients After COVID-19 Infection
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
Separates molecules based on size. Great for checking DNA after a Restriction Digest.

[BEFORE_START]
Have a DNA Sample ready, typically either from PCR or a recently performed Restriction Digest. Dilute down the 50X TAE Buffer to 1X.

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.