eamag/protocols-with-steps huggingface dataset:
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Insulin and glucagon hormones were detected in paraffin sections (4 μm thickness) of formalin-fixed human pancreases. Insulin and glucagon were detected immunohistochemically using mouse anti-human insulin mAb (ascites; Sigma) and mouse anti-porcine glucagon mAb (ascites; Sigma), respectively, with biotinylated anti-mouse IgG and avidin-biotin-complex (ABC reagent; PK-2200, Vector Labs). Background staining was checked using the corresponding isotype control Ig instead of the primary antibody. 3-amino-9-ethylcarbazole (AEC) was used as the chromogen. Stained sections were imaged using a light microscope with attached camera (Olympus BX41).</p>

[BEFORE_START]
<p>Materials:</p>
<p> </p>
<ol>
<li>Prepare graded alcohols and xylene for deparaffinizing tissue sections: 2 x xylene (250 ml/slide container), 2 x absolute ethanol (250 ml/slide container), 1 x 90% ethanol (250 ml), 1 x 70% ethanol (250 ml).</li>
</ol>
<p> </p>
<ol start="2">
<li>Prepare acetate buffer components:</li>
</ol>
<p>(i) 0.1N acetic acid: 290 μl glacial acetic acid in 50 ml deionized water</p>
<p>(ii) 0.1M sodium acetate: 410 mg anhydrous CH<sub>3</sub>COONa in 50 ml deionized water.</p>
<p>Prepare 0.1M acetate buffer (pH 5.2) by mixing 10.5 ml 0.1N acetic acid and 39.5 ml 0.1M sodium acetate.</p>
<p> </p>
<ol start="3">
<li>Prepare 18% (w/v) sodium azide.<br /><br /><br /></li>
<li>Prepare stock solution of 3-amino-9-ethylcarbazole (AEC; chromogen, 8 mg/ml: 40 mg AEC in 5 ml N-N-dimethyl formamide; protect from light and refrigerate at 4°C.<br /><br /><br /></li>
<li>Prepare M.O.M. diluent: 200 μl M.O.M. protein concentrate stock solution (Immunodetection kit, PK-2200, Vector) in 2.5 ml phosphate-buffered saline (PBS) for use either as a blocking step to minimize non-specific Ig binding or for diluting antibodies.<br /><br /><br /></li>
<li>Mabs and pAbs:</li>
</ol>
<p>Mouse anti-human insulin mAb (ascites), Sigma-Aldrich #12018</p>
<p>Mouse anti-porcine glucagon mAb (ascites), Sigma-Aldrich #G2654</p>
<p>Biotinylated anti-mouse IgG, M.O.M immunodetection kit, Vector Labs # PK-2200</p>
<p>Mouse IgG<sub>1Κ </sub>, eBioscience # 14-4714-85</p>
<p> </p>
<p> </p>
<p> </p>
<p>      7. Other reagents:</p>
<p>Hydrogen peroxide (30% w/w), Chem-Supply Pty Ltd (Australia) #HA154-500M<br /> Methanol, Merck #106009<br /> Sodium azide, Sigma-Aldrich #S2002<br /> 3-Amino-9-ethylcarbazole (AEC), Sigma-Aldrich #A5754<br /> Animal free blocker, Vector Labs #SP-5030<br /> Stock protein concentrate, M.O.M immunodetection kit, Vector Labs # PK-2200<br /> Avidin-biotin complex (ABC), M.O.M immunodetection kit, Vector Labs # PK-2200<br /> N-N-dimethyl formamide, Sigma #D158550<br /> Glycergel mounting medium, Dako #C0563</p>

[STEPS]
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TITLE: GenXZ Keto Gummies *Read 8 Facts*100% Clinically Certified Ingredients!
AUTHORS: GenXZ Keto Gummies

[DESCRIPTION]
GenXZ Keto Gummies is an excellent choice for everybody for losing weight that helps in reducing the extra fat from the body tone!
BUY NOW:- https://www.elitegross.com/buygenxzketogummies

[STEPS]
TITLE: Sample Collection and Measurement of Serum Neurofilament Light (NfL)
AUTHORS: Huw  Morris, Donald Grosset, Amanda Heslegrave, Henrik Zetterberg

[DESCRIPTION]
This protocol details the steps for the preparation of serum from human blood samples and the measurement of NfL concentration using the NF-Light Advantage kit on the HD-X Analyzer (Quanterix, Billerica, MA).

[BEFORE_START]
Check the planned procedure is covered by existing Research Ethics Committee approval and other required approvals.
Check that you have the correct personal protective equipment, supplies and consumables.

[STEPS]
SECTION: Blood Sample Collection and Serum Preparation
1. At enrolment, collect 10 mL of venous blood from each participant in Serum Separator Tubes (SST): https://www.fishersci.co.uk/shop/products/vacutainer-sst-ii-advance-tubes/12927696
 

SECTION: Blood Sample Collection and Serum Preparation
2. Within 1 hour of collection, centrifuge the SSTs at 2,500g for 15 minutes at room temperature
SECTION: Blood Sample Collection and Serum Preparation
3. Print cryogenic labels / barcodes and apply to cryotubes: https://www.brady.co.uk/labels
SECTION: Blood Sample Collection and Serum Preparation
4. Aliquot approx.  -0.5 mL serum into labelled cryotubes, box and/or rack then store at -80 °C.

SECTION: Measurement of Serum NfL Concentration
5. 
Measure serum NfL concentration using the Simoa® NF-light Advantage Kit for HD-1 / HD-X following the manufacturers instructions : https://www.quanterix.com/wp-content/uploads/2020/12/NF-light-Data-Sheet-HD-1%E2%88%95HD-X-2.pdf 
This kit is a digital immunoassay for the quantitative determination of NfL in serum, plasma and CSF.
 

SECTION: Measurement of Serum NfL Concentration
6. Run on the HD-X Analyzer (Quanterix, Billerica, MA), a fully automated Simoa® bead-based immunoassay platform: https://www.quanterix.com/instruments/simoa-hd-x-analyzer/
TITLE: PCR Reaction Optimization
AUTHORS: Kenneth Schackart

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">How to run nucleic acid amplification using the Thermo scientific PCR Master Mix kit.</div><div class = "text-block">Each reaction produces 50 μL.</div><div class = "text-block"><span>For the original protocol, look at: </span><a style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;"> </span></a><span style = ":;">.</span></div></div>

[STEPS]
?. [Prepare Work Area]
Spray entire work area with 70% EtOH including pipettes, tip holder used for holding PCR tubes, and work surface. Wipe with a paper towel.
?. [Prepare Work Area]
Spray entire work area with RNAway.
?. [Gather Materials]
Take styrofoam container to Marley 527 (directly across from Marley 509) and fill halfway with ice.
?. [Gather Materials]
Set PCR tube holder on ice, and allow to cool for .
?. [Gather Materials]
Transfer Master Mix, primers, RNAse free water, and target tubes from freezer to PCR tube holder on ice.
?. [Gather Materials]
Allow reagents to thaw on ice
?. [Gather Materials]
Carefully obtain (2) 0.2 mL PCR tubes. Label one with "NTC" and the other "TARG". These will be your reaction vessels.
To avoid contamination when grabbing PCR tubes, only touch the outside of tubes. Avoid touching the inside of the caps of other tubes in this process. This is critical.
?. [Prepare Reaction]
Add the following to your two tubes:  ABC1TargetNTC2PCR Master Mix25 μL25 μL3Primer Mixture (25 μM)0.2 - 2.0 μL0.2 - 2.0 μL4Target1 μL-5Waterto 50 μLto 50 μL6Total50 μL50 μLVarious primer concentrations are to be optimized. Start with 1.0 μL (0.5 μM).
ABC1TargetNTC2PCR Master Mix25 μL25 μL3Primer Mixture (25 μM)0.2 - 2.0 μL0.2 - 2.0 μL4Target1 μL-5Waterto 50 μLto 50 μL6Total50 μL50 μL
?. [Gather Materials]
Vortex mix all reagents for approximately .
?. [Prepare Reaction]
Vortex mix the reaction mixture.
?. [Prepare Reaction]
Spin down reaction mixture.
?. [Run PCR Reaction]
Place reaction vessels into thermocycler.
?. [Run PCR Reaction]
Turn on thermocycler
?. [Run PCR Reaction]
Hit PROCEED to select a reaction cycle.
?. [Run PCR Reaction]
Scroll using the '' keys to get to EPCR.Begin using the following program:  ABCD1StepTemperatureTimeNumber Cycles2Denaturation95ºC3 min13Denaturation95ºC30 s304Annealing58ºC30 s5Extension72ºC60 s6Final Extension72º10 min1
ABCD1StepTemperatureTimeNumber Cycles2Denaturation95ºC3 min13Denaturation95ºC30 s304Annealing58ºC30 s5Extension72ºC60 s6Final Extension72º10 min1
?. [Run PCR Reaction]
Press PROCEED to begin
?. [Gather Materials]
Spin down all reagents for approximately .
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
This is a highly reproducible method for creating hit science videos. Guaranteed to lead to millions of views, fame, faculty position, tenure, and stable funding.<br /><br />* Legal disclaimer: This is not a peer reviewed protocol. Therefore, all claims should be taken with as much skepticism as anything that is peer reviewed.

[STEPS]
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TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>These protocols accompany the following <em>GigaScience</em> publication:</p>
<p> </p>
<p>Jihoon Jo, et al.  (2016): Draft genome of the sea cucumber Apostichopus japonicus and genetic polymorphism among color variants. <em>GigaScience</em>...</p>

[STEPS]
TITLE: LRRK2-RCKW: MLi-2: E11 DARPin cryo-EM sample preparation
AUTHORS: Marta Sanz Murillo, Andres Leschziner

[DESCRIPTION]
Protocol used to prepare LRRK2-RCKW: DARPin:MLi-2 complex and cryo-EM grid preparation.

[BEFORE_START]
Make 

[STEPS]
SECTION: Protein purification and buffer exchange
1. His6-Z-TEV-LRRK2-RCKW was expressed and purified as described in a previous protocol.

 


SECTION: E11 DARPin purification
9. E11 DARPin purified as described in the next protocol
SECTION: cryo-EM sample preparation
10. We used UltraAuFoil Holey Gold 2/2 200 mesh grids and plasma cleaning them in the Solarus II (Gatan) using the QuantiFoil Au preset.
SECTION: Protein purification and buffer exchange
2. Prepare LRRK2 buffer exchange. Keep it at 4ºC.
20 millimolar (mM) HEPES pH=7.4
150 millimolar (mM) NaCl
2.5 millimolar (mM) MgCl2
20 micromolar (µM) GDP
0.5 millimolar (mM) TCEP
SECTION: Protein purification and buffer exchange
3. Spin down purified LRRK2-RCKW (10000 rcf, 4°C, 10 minutes). Leave protein on ice afterward.
For the best result, keep protein on ice and reduce the amount of time between spinning
and freezing cryo-EM samples.
SECTION: Protein purification and buffer exchange
4. Exchange buffer using a spin desalting column (Zeba‱ Spin Desalting Columns, 7K MWCO (Catalog number: 89877). 
SECTION: Protein purification and buffer exchange
5. Spin down again the exchange buffer LRRK2-RCKW (10000 rcf, 4°C, 10 minutes) and measure the concentration. Leave protein on ice afterward
 

SECTION: Protein purification and buffer exchange
7. Based on LRRK2-RCKW concentration, add the necessary volume to get a proportional ratio
LRRK2:DARPin:MLi-2 1:1.25:3 and dilute to a final 10 micromolar (µM) LRRK2-RCKW concentration using exchange buffer (150 mM NaCl).
SECTION: Protein purification and buffer exchange
5.1. Thaw E11 DARPin and spin it down. Measure its concentration.
SECTION: Protein purification and buffer exchange
8. Incubate 10 minutes at RT. Afterward, keep it on ice until grid preparation.
SECTION: Protein purification and buffer exchange
6. Dilute MLi-2 stock (diluted in 100% DMSO) to a desired concentration 
SECTION: cryo-EM sample preparation
11. Dilute the sample to the desired concentration using the LRRK2 exchange buffer. We used 6 micromolar (µM).
SECTION: cryo-EM sample preparation
12. Apply 3 to 3.5 microliters (µl) of sample and plunge freeze. We used a
Vitrobot (FEI) to blot away excess sample and plunge freeze in ethane liquid. (In
our case, we use 4 seconds as a time blot as 20 sec as a wait time and 4 as a blot force, but these
parameters are slightly different from one Vitrobot to another. I would try
with the Vitrobot parameters already tested in your machine first).

SECTION: cryo-EM sample preparation
13. Store grids in liquid nitrogen until ready for imaging
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This protocol is to fix freshly collected plant tissue for serial sectioning. Although this protocol is similar to ones used for <em>in situ</em> mRNA hybridization, it should not be used for that purpose as it is not nearly careful/gently enough. This protocol has been tested with tomato, tobacco, and date fruits with good results.</p>

[BEFORE_START]
<p>Label tubes using an ultrapermanent marker with the sample information (e.g. “3 DPA, Plant #1”), or alternatively, write a label with pencil and paper (not pen) and place it in the container with the sample.</p>

[GUIDELINES]
<p>Tissue collected for this procedure should be small in order to inflitrate well. Samples approaching 1cm<sup>3</sup> may need to be cut apart or scored in order to let the fixative penatrate well. Plant organs with a continuous and/or waterproof membrane, like small fruits, should also be scored.</p>

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TITLE: Automated Tissue Fixation with 10% NBF (Leica)
AUTHORS: Donna Skinner, Teri Bowman, Jon Aster

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Human tissue specimens are frequently formalin-fixed and paraffin-embedded (FFPE) for histological staining,  pathological review, and archival purposes. This is the routine fixation protocol used by the Specialized Histopathology (SHP) Core and the Department of Pathology at Brigham and Women's Hospital. The protocol uses the Leica ASP300 S Fully Enclosed Tissue Processor to automate fixation.</div></div>

[STEPS]
?. [Automated Tissue Fixation with 10% NBF (Leica)]
Formalin Short ProgramStation/ReagentTimeTempP/V10% NBF00:12 min40Y70% Ethyl Alcohol00:15 min40Y95% Ethyl Alcohol00:15 min40Y95% Ethyl Alcohol00:20 min40Y100% Ethyl Alcohol00:20 min40Y100% Ethyl Alcohol00:20 min40YXylene00:20 min40YXylene00:30 min40YParaffin Wax 100:05 min60YParaffin Wax 200:10 min60YParaffin Wax 300:15 min60Y
Station/ReagentTimeTempP/V10% NBF00:12 min40Y70% Ethyl Alcohol00:15 min40Y95% Ethyl Alcohol00:15 min40Y95% Ethyl Alcohol00:20 min40Y100% Ethyl Alcohol00:20 min40Y100% Ethyl Alcohol00:20 min40YXylene00:20 min40YXylene00:30 min40YParaffin Wax 100:05 min60YParaffin Wax 200:10 min60YParaffin Wax 300:15 min60Y
?. [Automated Tissue Fixation with 10% NBF (Leica)]
Program the Leica ASP300 S Fully Enclosed Tissue Processor with either the short program or long program depending on size, thickness, and type of sample.
?. [Automated Tissue Fixation with 10% NBF (Leica)]
Formalin Long ProgramStation/ReagentTimeTempP/V10% NBF02:00 hr40Y70% Ethyl Alcohol00:45 min40Y95% Ethyl Alcohol00:45 min40Y95% Ethyl Alcohol00:45 min40Y100% Ethyl Alcohol00:30 min40Y100% Ethyl Alcohol01:00 min40Y100% Ethyl Alcohol30 min40YXylene30 min40YXylene30 min40YParaffin Wax 130 min60YParaffin Wax 230 min60YParaffin Wax 330 min60Y
Station/ReagentTimeTempP/V10% NBF02:00 hr40Y70% Ethyl Alcohol00:45 min40Y95% Ethyl Alcohol00:45 min40Y95% Ethyl Alcohol00:45 min40Y100% Ethyl Alcohol00:30 min40Y100% Ethyl Alcohol01:00 min40Y100% Ethyl Alcohol30 min40YXylene30 min40YXylene30 min40YParaffin Wax 130 min60YParaffin Wax 230 min60YParaffin Wax 330 min60Y
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.</p>
<p> </p>
<p>Adapted from the original publication Stein, J (ED.) 1973. Handbook of Phycological methods. Culture methods and growth measurements. Cambridge University Press. 448 pp.</p>

[STEPS]
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TITLE: Cell-based Tau seeding assay
AUTHORS: Patricia Yuste-Checa

[DESCRIPTION]
This protocol details the procedure of cell-based Tau seeding assay.

[GUIDELINES]
Banning, C. et al. A flow cytometry-based FRET assay to identify and analyse proteinprotein interactions in living cells. PLoS One 5, e9344, doi:10.1371/journal.pone.0009344 (2010).

[STEPS]
1. Plate 100,000 cells per well of HEK293T FRET reporter cell line (TauRD-YT, FLTau-YT or commercial cell line Tau RD P301S FRET Biosensor (ATCC, CRL- 3275)) into a 12-well plate.
 

2. On the next day, seed aggregation in cells with Tau aggregates.
 

2.1. Seeding with transfection reagent:

Prepare lipofectamine mix for n+1 samples: 50 µL Opti-MEM (Gibco) + 1.6 µL lipofectamine 3000 reagent (Thermo Fisher Scientific) per sample.
Distribute 51.6 µL of the lipofectamine mix to individual tubes.
Add corresponding amount of Tau aggregates.
 



2.2. Seeding without transfection reagent:

Mix Tau aggregates with 500 µL fresh media.
Replace cell media with this mix.
 


3. Collect and quantify FRET positive cells after 960 min-1200 min (when using lipofectamine) or 2880 min (without lipofectamine or when using the FLTau-YT reporter cells):

3.1. Wash cells with PBS.
3.2. Add 100 µL TrypL Express Enzyme (Gibco).

3.3. Collect the cells adding 400 µL of media and transfer them to an Eppendorf tube.

3.4. Centrifuge at 1000 x g for 5 min.

3.5. Discard the supernatant and wash the cell pellet with 200 µL PBS.

3.6. Centrifuge at 1000 x g for 5 min.

3.7. Discard supernatants and resuspend the cell pellet in 200 µL PBS.
 


3.8. Quantify FRET positive cells by flow cytometry. A minimum of 50,000 single cells should be analyzed. The FRET positive gate is set by plotting the FRET fluorescence signal versus the mTurquoise2 fluorescence signal using as reference nonseeded cells (See Figure below).
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
Case 1: Quick check of viral concentration. Use this protocol when you can count your samples in a few days.<br /><br />For&nbsp;when long term storage of slide is required, see <a href="https://www.protocols.io/view/SYBR-Gold-Staining-for-Viral-Enumeration-Case-2-c7fzjm" target="_blank">Case 2</a>.&nbsp;

[STEPS]
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TITLE: iGEM Calibration Protocol - Flow Cytometry Cell Size
AUTHORS: Jacob Beal, Cheryl Telmer, Richard Tennant, Paul Rutten

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Conversion from forward scatter to Eμm is not a linear function, so data cannot be converted simply by multiplying with a scaling factor. We thus recommend use of software tools for processing data with size calibration.</div><div class = "text-block">This protocol can be combined with bead-based fluorescence calibration.</div></div>

[STEPS]
?. Prepare and culture experimental samples according to your desired experimental procedure.
?. Prepare experimental samples as needed for running through your flow cytometer.
?. Follow SpheroTech directions for preparation for PPS-6K bead sample.
?. Measure all samples in flow cytometer Using the bead sample, adjust FSC channel voltage so that the top bead peak is below detector saturation.Using the negative process control sample, adjust forward-scatter and side-scatter voltages to place the strong cell peak as close to the center of the detector range as possible (see example illustrated below).  Instrument gating should be set to ensure that no cell events are discarded (see example illustrated below). Collect at least 10,000 events per sample.Using the negative process control sample, adjust forward-scatter and side-scatter voltages to place the strong cell peak as close to the center of the detector range as possible (see example illustrated below).  Instrument gating should be set to ensure that no cell events are discarded (see example illustrated below). Collect at least 10,000 events per sample.Instrument gating should be set to ensure that no cell events are discarded (see example illustrated below). Collect at least 10,000 events per sample.
?. Compute the Eμm/a.u. scaling function from bead sample using TASBE Flow Analytics to create a TASBE color model.
?. Convert experimental data to Eμm using TASBE Flow Analytics analysis functions.
TITLE: Western blotting to detect ATP13A2 and ATP13A3
AUTHORS: Marine Houdou, Peter Vangheluwe

[DESCRIPTION]
Protocol to detect ATP13A2 and ATP13A3 via Western Blotting.

[STEPS]
SECTION: Harvesting cells
1. Depending on cell type, collect the cells by scrapping them with a scrapper in Dulbecco’s Phosphate Buffered saline modified without calcium chloride and magnesium chloride (DPBS) (SH-SY5Y) or, using 0.25% Trypsin-EDTA (HMEC-1) for which stop enzymatic reaction by adding culture medium.
SECTION: Harvesting cells
2. Centrifuge cell suspensions at 450 g (SH-SY5Y) or 2500 rpm (HMEC-1), 4°C for 5 min.

SECTION: Harvesting cells
3. Resuspend cell pellets with DPBS and centrifuge following the same indications as in 2. Repeat once.
SECTION: Harvesting cells
4. Discard supernatants and keep cell pellets on ice.
SECTION: Cell lysis and protein concentration determination
5. Resuspend cell pellets in RIPA buffer (RIPA Lysis and Extraction Buffer supplemented with protease cocktail inhibitors. 
SECTION: Cell lysis and protein concentration determination
6. Vortex 30 s and keep on ice for 30 min.

SECTION: Cell lysis and protein concentration determination
7. Centrifuge at 20,000g, 4°C for 30 min.

SECTION: Cell lysis and protein concentration determination
8. Keep supernatants on ice to proceed with protein concentration determination using the micro-BCA Protein Assay Kit.
SECTION: SDS-PAGE
9. Loading
SECTION: SDS-PAGE
9.1. Mix 20 µg of protein with NuPAGE LDS sample buffer and 5% β-mercaptoethanol final.
SECTION: SDS-PAGE
9.2. For this specific protocol to detect ATP13A2 and ATP13A3, do not boil samples.
SECTION: SDS-PAGE
9.3. Load protein on pre-cast 4-12% Bis-Tris gels. Include at least one lane with a protein ladder.
SECTION: SDS-PAGE
10. Running
SECTION: SDS-PAGE
10.1. Run for 10 minat 100V and 90 minat 110-130V.

SECTION: SDS-PAGE
11. Transfer
SECTION: SDS-PAGE
11.1. Transfer onto PVDF membranes using a liquid transfer and following settings: 100V, 75 min, 4°C.

SECTION: SDS-PAGE
12. Ponceau staining
SECTION: SDS-PAGE
12.1. Rinse membrane with distilled water.
SECTION: SDS-PAGE
12.2. Incubate membrane with Ponceau staining for 5 min, 19 rpm.
SECTION: SDS-PAGE
13. Blocking
SECTION: SDS-PAGE
13.1. 60 min Block membranes with blocking buffer (5% milk powder in 1X TBS and 0.1% Tween20 (REF)) for 60 min at room temperature, 19 rpm.

SECTION: SDS-PAGE
14. Primary antibodies
SECTION: SDS-PAGE
14.1. Incubate membrane with primary antibodies in solution (1% bovine serum albumin in 1X TBS-Tween20 (TBS-T) buffer), 60 minat 4°C, 19 rpm.

SECTION: SDS-PAGE
14.2. Wash membrane three times for 5 min in TBS-T, 19 rpm.

SECTION: SDS-PAGE
15. Secondary antibodies
SECTION: SDS-PAGE
15.1. Incubate membrane with peroxidase-conjugated secondary antibodies in solution (1% milk powder in 1X TBS-T) for 60 minat room temperature and, 19 rpm.

SECTION: SDS-PAGE
15.2. Wash membrane five times for5 minin TBS-T, 19 rpm.

SECTION: SDS-PAGE
16. Detection
SECTION: SDS-PAGE
17. Use a chemiluminescence reagent to detect signal and acquire with a Biorad Camera (Vilber Lourmat) and its software (ImageLab).
SECTION: SDS-PAGE
12.3. Scan membrane if necessary.