TITLE: Active Avoidance protocol 01282020
AUTHORS: Teresa Macheda, Henry Snider, James B. Watson, Kelly N. Roberts, Adam Bachstetter

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:justify"></div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:left"><span style = ":UNDERLINE;">Active Avoidance Test Using Shock as unconditioned stimulus</span></div></div><div class = "text-block"><div class = "justify" style = "text-align:left"></div></div><div class = "text-block"><div class = "justify" style = "text-align:left">Active avoidance is a widely-used paradigm to evaluate fear-motivated associative learning and memory. </div></div><div class = "text-block"><div class = "justify" style = "text-align:left"></div></div><div class = "text-block"><div class = "justify" style = "text-align:left">The Gemini shuttle box was used to perform the active avoidance test. In the active avoidance behavior, a subject is trained to avoid an aversive unconditioned stimulus (US), by associating the conditioned stimulus (CS: light and/or auditory tone) with the foot-shock (US). Over repeated trials, the subject learns that to avoid the foot-shock (US) it must shuttle between chambers when the warning cue (CS) is presented.  Learning performance is evaluated by analysis of avoidance behavior during the test.</div></div><div class = "text-block"><div class = "justify" style = "text-align:left"></div></div><div class = "text-block"><div class = "justify" style = "text-align:left">Five days test: </div></div><div class = "text-block"><div class = "justify" style = "text-align:left"><span>The mouse will be placed in the dark box with the guillotine door open, free to explore both sides of the chamber for 5 minutes. Then a </span><span style = "font-weight:bold;font-style:italic;">cue -house light (1864 lux)- </span><span>will be presented for 10 seconds. The mouse could step into the safe side (no shock delivered), otherwise a foot shock would be delivered </span><span style = "font-weight:bold;">(e.g. 0.2 mA, 2 sec</span><span>). The mouse will be exposed to 50 trials/day (ITT 30±5 sec) for a total of 5 days. CS opposite side of US side.</span></div></div><div class = "text-block"><div class = "justify" style = "text-align:left"></div></div><div class = "text-block"><div class = "justify" style = "text-align:left"><span style = "font-weight:bold;">Habituation=300 sec</span></div></div><div class = "text-block"><div class = "justify" style = "text-align:left"><span style = "font-weight:bold;">CS= housing light, 10 sec</span></div></div><div class = "text-block"><div class = "justify" style = "text-align:left"><span style = "font-weight:bold;">US= 0.2 mA (e.g. ) shock, 2 sec</span></div></div><div class = "text-block"><div class = "justify" style = "text-align:left"><span style = "font-weight:bold;">ITT=30±5 sec</span><span style = "font-weight:bold;"></span></div></div><div class = "text-block"><div class = "justify" style = "text-align:left"><span style = "font-weight:bold;"># Trials= 50</span></div></div><div class = "text-block"><div class = "justify" style = "text-align:left"><span style = "font-weight:bold;"># days=5</span></div></div></div>

[STEPS]
?. Experiment Design and Recommendations:Housing: Behavior is affected by the housing mode. Singly housed mice could perform differently than group housed mice. Blinding: Experimental groups of mice should be coded while the operator is testing the mice.Test order: Mice should be randomized in test order, to avoid confounded results if all the mice from the same experimental group are tested before all the mice from a different group. The randomization should be applied in all the cohorts tested across time.Time of testing: Time of day strongly affects performance. Testing should be conducted during the same time between days and experiments. If a study requires more animals, several cohorts should be tested. It is recommended that each cohort of mice is tested starting the same day of the week and by the same personnel to reduce the introduction of new variability in the task. Sex: Males and females should be tested in separate groups, in particular in different cohorts. If this is not possible and more than one active avoidance chamber is available make sure to dedicate one chamber for each sex.
?. Set upComputer setupTurn on the computerMake sure that the Avoidance system is plugged in and turned onOpen the avoidance system program  (GEMINI.exe)Click file-> click create a new study database   Name your DATABASE making sure to leave the m-defined extension .mdb and click Open  A "connecting" window will pop pup, click OK  Defining a ProtocolMake your protocol, click "Add Protocol Record"  e.g.         Protocol ID: active avoidance        CS Type: House        US Type: Shock        Gate: Open        Misc.: Assume location/ Co-Terminate CS/US                Trial type: Active        Acclimation (sec): 300        Accl Intrvl (sec): 30        CS Duration (sec): 10        US Duration (sec): 2        Max trial  Length (sec): 12 -this will be calculated automatically         Max Num Trial: 50        ITI= 30±5 sec will be generated by the software following this steps:                click "Add ITI Record  -> name it                Min (sec): 25                Max (sec): 35                Delta (sec): 5                Quantity: 49                -click Generate RandomSave protocol record          How to open study database/ protocolClick file-> click open existing study database   Choose your database and click open. The database contains any previously defined protocols. The protocol of your choice will be ready to be applied to your study  How to save your fileClick file, then “save as”How to add subjects to the experimentOnce the protocol is defined, you can add subjects to your experiment.Click triangle-star and pen button on top left side (you can create a session and add subjects)    Click Session  Click Add session record, Fill in Session ID (e.g. name , date)  Click OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedMake sure that the Avoidance system is plugged in and turned onOpen the avoidance system program  (GEMINI.exe)Click file-> click create a new study database   Name your DATABASE making sure to leave the m-defined extension .mdb and click Open  A "connecting" window will pop pup, click OK  Defining a ProtocolMake your protocol, click "Add Protocol Record"  e.g.         Protocol ID: active avoidance        CS Type: House        US Type: Shock        Gate: Open        Misc.: Assume location/ Co-Terminate CS/US                Trial type: Active        Acclimation (sec): 300        Accl Intrvl (sec): 30        CS Duration (sec): 10        US Duration (sec): 2        Max trial  Length (sec): 12 -this will be calculated automatically         Max Num Trial: 50        ITI= 30±5 sec will be generated by the software following this steps:                click "Add ITI Record  -> name it                Min (sec): 25                Max (sec): 35                Delta (sec): 5                Quantity: 49                -click Generate RandomSave protocol record          How to open study database/ protocolClick file-> click open existing study database   Choose your database and click open. The database contains any previously defined protocols. The protocol of your choice will be ready to be applied to your study  How to save your fileClick file, then “save as”How to add subjects to the experimentOnce the protocol is defined, you can add subjects to your experiment.Click triangle-star and pen button on top left side (you can create a session and add subjects)    Click Session  Click Add session record, Fill in Session ID (e.g. name , date)  Click OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedOpen the avoidance system program  (GEMINI.exe)Click file-> click create a new study database   Name your DATABASE making sure to leave the m-defined extension .mdb and click Open  A "connecting" window will pop pup, click OK  Defining a ProtocolMake your protocol, click "Add Protocol Record"  e.g.         Protocol ID: active avoidance        CS Type: House        US Type: Shock        Gate: Open        Misc.: Assume location/ Co-Terminate CS/US                Trial type: Active        Acclimation (sec): 300        Accl Intrvl (sec): 30        CS Duration (sec): 10        US Duration (sec): 2        Max trial  Length (sec): 12 -this will be calculated automatically         Max Num Trial: 50        ITI= 30±5 sec will be generated by the software following this steps:                click "Add ITI Record  -> name it                Min (sec): 25                Max (sec): 35                Delta (sec): 5                Quantity: 49                -click Generate RandomSave protocol record          How to open study database/ protocolClick file-> click open existing study database   Choose your database and click open. The database contains any previously defined protocols. The protocol of your choice will be ready to be applied to your study  How to save your fileClick file, then “save as”How to add subjects to the experimentOnce the protocol is defined, you can add subjects to your experiment.Click triangle-star and pen button on top left side (you can create a session and add subjects)    Click Session  Click Add session record, Fill in Session ID (e.g. name , date)  Click OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedClick file-> click create a new study database   Name your DATABASE making sure to leave the m-defined extension .mdb and click Open  A "connecting" window will pop pup, click OK  Defining a ProtocolMake your protocol, click "Add Protocol Record"  e.g.         Protocol ID: active avoidance        CS Type: House        US Type: Shock        Gate: Open        Misc.: Assume location/ Co-Terminate CS/US                Trial type: Active        Acclimation (sec): 300        Accl Intrvl (sec): 30        CS Duration (sec): 10        US Duration (sec): 2        Max trial  Length (sec): 12 -this will be calculated automatically         Max Num Trial: 50        ITI= 30±5 sec will be generated by the software following this steps:                click "Add ITI Record  -> name it                Min (sec): 25                Max (sec): 35                Delta (sec): 5                Quantity: 49                -click Generate RandomSave protocol record          How to open study database/ protocolClick file-> click open existing study database   Choose your database and click open. The database contains any previously defined protocols. The protocol of your choice will be ready to be applied to your study  How to save your fileClick file, then “save as”How to add subjects to the experimentOnce the protocol is defined, you can add subjects to your experiment.Click triangle-star and pen button on top left side (you can create a session and add subjects)    Click Session  Click Add session record, Fill in Session ID (e.g. name , date)  Click OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedName your DATABASE making sure to leave the m-defined extension .mdb and click OpenA "connecting" window will pop pup, click OK  Defining a ProtocolMake your protocol, click "Add Protocol Record"  e.g.         Protocol ID: active avoidance        CS Type: House        US Type: Shock        Gate: Open        Misc.: Assume location/ Co-Terminate CS/US                Trial type: Active        Acclimation (sec): 300        Accl Intrvl (sec): 30        CS Duration (sec): 10        US Duration (sec): 2        Max trial  Length (sec): 12 -this will be calculated automatically         Max Num Trial: 50        ITI= 30±5 sec will be generated by the software following this steps:                click "Add ITI Record  -> name it                Min (sec): 25                Max (sec): 35                Delta (sec): 5                Quantity: 49                -click Generate RandomSave protocol record          How to open study database/ protocolClick file-> click open existing study database   Choose your database and click open. The database contains any previously defined protocols. The protocol of your choice will be ready to be applied to your study  How to save your fileClick file, then “save as”How to add subjects to the experimentOnce the protocol is defined, you can add subjects to your experiment.Click triangle-star and pen button on top left side (you can create a session and add subjects)    Click Session  Click Add session record, Fill in Session ID (e.g. name , date)  Click OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedDefining a ProtocolMake your protocol, click "Add Protocol Record"  e.g.         Protocol ID: active avoidance        CS Type: House        US Type: Shock        Gate: Open        Misc.: Assume location/ Co-Terminate CS/US                Trial type: Active        Acclimation (sec): 300        Accl Intrvl (sec): 30        CS Duration (sec): 10        US Duration (sec): 2        Max trial  Length (sec): 12 -this will be calculated automatically         Max Num Trial: 50        ITI= 30±5 sec will be generated by the software following this steps:                click "Add ITI Record  -> name it                Min (sec): 25                Max (sec): 35                Delta (sec): 5                Quantity: 49                -click Generate RandomSave protocol record          How to open study database/ protocolClick file-> click open existing study database   Choose your database and click open. The database contains any previously defined protocols. The protocol of your choice will be ready to be applied to your study  How to save your fileClick file, then “save as”How to add subjects to the experimentOnce the protocol is defined, you can add subjects to your experiment.Click triangle-star and pen button on top left side (you can create a session and add subjects)    Click Session  Click Add session record, Fill in Session ID (e.g. name , date)  Click OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordede.g.         Protocol ID: active avoidance        CS Type: House        US Type: Shock        Gate: Open        Misc.: Assume location/ Co-Terminate CS/US        Trial type: Active        Acclimation (sec): 300        Accl Intrvl (sec): 30        CS Duration (sec): 10        US Duration (sec): 2        Max trial  Length (sec): 12 -this will be calculated automatically         Max Num Trial: 50        ITI= 30±5 sec will be generated by the software following this steps:                click "Add ITI Record  -> name it                Min (sec): 25                Max (sec): 35                Delta (sec): 5                Quantity: 49                -click Generate RandomSave protocol record          How to open study database/ protocolClick file-> click open existing study database   Choose your database and click open. The database contains any previously defined protocols. The protocol of your choice will be ready to be applied to your study  How to save your fileClick file, then “save as”How to add subjects to the experimentOnce the protocol is defined, you can add subjects to your experiment.Click triangle-star and pen button on top left side (you can create a session and add subjects)    Click Session  Click Add session record, Fill in Session ID (e.g. name , date)  Click OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedHow to open study database/ protocolClick file-> click open existing study database   Choose your database and click open. The database contains any previously defined protocols. The protocol of your choice will be ready to be applied to your study  How to save your fileClick file, then “save as”How to add subjects to the experimentOnce the protocol is defined, you can add subjects to your experiment.Click triangle-star and pen button on top left side (you can create a session and add subjects)    Click Session  Click Add session record, Fill in Session ID (e.g. name , date)  Click OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedChoose your database and click open. The database contains any previously defined protocols. The protocol of your choice will be ready to be applied to your study  How to save your fileClick file, then “save as”How to add subjects to the experimentOnce the protocol is defined, you can add subjects to your experiment.Click triangle-star and pen button on top left side (you can create a session and add subjects)    Click Session  Click Add session record, Fill in Session ID (e.g. name , date)  Click OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedHow to save your fileClick file, then “save as”How to add subjects to the experimentOnce the protocol is defined, you can add subjects to your experiment.Click triangle-star and pen button on top left side (you can create a session and add subjects)    Click Session  Click Add session record, Fill in Session ID (e.g. name , date)  Click OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedHow to add subjects to the experimentOnce the protocol is defined, you can add subjects to your experiment.Click triangle-star and pen button on top left side (you can create a session and add subjects)    Click Session  Click Add session record, Fill in Session ID (e.g. name , date)  Click OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedClick Session  Click Add session record, Fill in Session ID (e.g. name , date)  Click OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedClick Add session record, Fill in Session ID (e.g. name , date)  Click OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedClick OKUnder “Protocol ID,” select the appropriate protocolClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedClick Subjects  Fill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedFill in group ID, and group order (you can just put a random #), then click add subject record    If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recorded If the animal ID is already saved, click  Subject ID  and select your subjectSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedSelect the station (it is important to assign the subject to the correct station/chamber when you use more than one box)  Click Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedClick Preload session to trial, data will be preloaded and experiment will be able to start  Click Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recordedClick Run -> click Trial -> Type in your name in the “user login” box that pops up   Now mice can be  loaded in the gemini box, push the "start" button on the box, trial will start and data will be recorded
?. Experimental ProcedureBefore beginning, clean the chamber floor and walls Place bedding in the tray beneath the grid floorPlace the mouse in one of the two compartments (Right or left side)Shut the doorPress start on the Gemini box: after 5 minutes of habituation, the mouse will be tested in a total of 50 trialsWhen the mouse is finished it may be returned to its home cageClean the chamber floor and walls between each mouse in order to maintain a consistent intensity of shock released from the floorPlace next mouse in the chamber and continue with steps 1-7Repeat 1-7 every day for 5 days
?. Export dataClick Reports  Select the data you want to export under Session ID -> Click Export to Excel File     Select the file -> click OK  Name your file -> click openSelect the data you want to export under Session ID -> Click Export to Excel File     Select the file -> click OK  Name your file -> click open   Select the file -> click OK  Name your file -> click open
?. Cleaning upClean the chamber floor and walls Empty the chamber tray of bedding, and clean the drawer Return mice to their home rooms
?. How to check if the chamber works properlyComputer setupTurn on the computerMake sure that the Avoidance system is plugged in and turned onOpen the avoidance system program  (GEMINI.exe)Click Run -> click Diagnostics  Click on the chamber controls to evaluate if the chamber is working properlyMake sure that the Avoidance system is plugged in and turned onOpen the avoidance system program  (GEMINI.exe)Click Run -> click Diagnostics  Click on the chamber controls to evaluate if the chamber is working properlyOpen the avoidance system program  (GEMINI.exe)Click Run -> click Diagnostics  Click on the chamber controls to evaluate if the chamber is working properlyClick Run -> click Diagnostics  Click on the chamber controls to evaluate if the chamber is working properly
?. Setting up the gemini boxActive Avoidance Maintenance:  Each testing day, the lights and shock should be tested before the start of each testing session.Active Avoidance box should be cleaned between subjects, in particular the chamber floor. The presence of urine will increase the shock intensity experienced by the animal. Turn on the boxSet up the shock intensity to use on the box -> put the switcher PROGRAM/ MANUAL on MANUAL -> put the switcher RUN/ADJUST on Adjust ->  adjust current using the CURRENT ADJUST knob to the shock to use during the test (you can read the value on the SHOCK INTENSITY (mA) display) -> Switch the switcher RUN/ADJUST in the centerTurn on the boxSet up the shock intensity to use on the box -> put the switcher PROGRAM/ MANUAL on MANUAL -> put the switcher RUN/ADJUST on Adjust ->  adjust current using the CURRENT ADJUST knob to the shock to use during the test (you can read the value on the SHOCK INTENSITY (mA) display) -> Switch the switcher RUN/ADJUST in the center
?. Export dataClick Reports  Select "Session ID"  Click "Export to Excel File" and select on the Dialog window the file you want to export and click OK   Select the folder where you want to save your file, name the file and click "Open"  Example of excel file, 4-worksheet: Select "Session ID"  Click "Export to Excel File" and select on the Dialog window the file you want to export and click OK   Select the folder where you want to save your file, name the file and click "Open"  Example of excel file, 4-worksheet: Click "Export to Excel File" and select on the Dialog window the file you want to export and click OK   Select the folder where you want to save your file, name the file and click "Open"  Example of excel file, 4-worksheet: Select the folder where you want to save your file, name the file and click "Open"  Example of excel file, 4-worksheet:  Session Results -> Subject ID, trials, latency and results (avoided, escaped or No Response) are reported in this worksheet.Acclimation -> number of crossing during acclimation time.InterTrialInterval -> inter trial time (seconds) and number of crossing during this interval.Protocol -> info about your session and protocol are saved in this worksheet.