TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p dir="ltr" style="line-height: 1.3800000000000001; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">Scott, JA, WG brogdon, and FH Collins, </span><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-style: italic; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">Identification of single specimens of the Anopholes gambiae complex by the polymerase chain reaction.</span><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;"> Am J Trop Med Hyg, 1993. </span><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">49</span><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">(4): p. 520-9</span></p>
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[BEFORE_START]
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<table style="border: none; border-collapse: collapse; height: 194px;" width="713"><colgroup><col width="217" /><col width="228" /><col width="108" /><col width="48" /></colgroup>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Cambria; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">PRIMERS – 5’ TO 3’</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Cambria; color: #000000; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">Size of fragment (BP)</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Cambria; color: #000000; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">Tm</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Cambria; color: #000000; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">Universal (forward)</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">GTG  TGC  CCC  TTC   CTC   GAT  GT</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">58.3</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Cambria; color: #000000; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">GA – gambiae (reverse)</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">CTG  GTT  TGG  TCG  GCA  CGT   TT</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">        390</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">59.3</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Cambria; color: #000000; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">ME – merus &amp; melus (reverse)</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">TGA  CCA  ACC  CAC  TCC   CTT   GA</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">        466/464</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">57.2</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Cambria; color: #000000; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">AR – arabiensis (reverse)</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">AAG  TGT  CCT  TCT  CCA   TCC   TA</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">        315</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">47.4</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Cambria; color: #000000; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">QD – quadriannulatus (reverse)</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">CAG  ACC  AAG  ATG  GTT  AGT  AT</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">        153</span></p>
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<p dir="ltr" style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14.6667px; font-family: Calibri; color: #000000; font-weight: bold; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">42.7</span></p>
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[GUIDELINES]
<br />
<div dir="ltr" style="margin-left: -5.75pt;"> Guideline for Identification<br /><br />M Form: 370bp<br />S Form: 260bp</div>

[STEPS]
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