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[GUIDELINES]
The following is a list of antibody concentrations for use with the protocol 'Immunohistochemistry - Drosophila Embryo'<br />Custom antibodies were provided by the published authors.<br /><br />DSHB Antibodies<br /><table style='border-collapse: collapse; width: 853pt;' border='0' width='853' cellspacing='0' cellpadding='0'><tbody><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; width: 114pt;' width='114' height='15'>Antigen</td><td class='xl64' style='border-left: none; width: 69pt;' width='69'>Host Species</td><td class='xl64' style='border-left: none; width: 168pt;' width='168'>Product ID</td><td class='xl65' style='border-left: none; width: 77pt;' width='77'>Concentration</td><td class='xl64' style='border-left: none; width: 425pt;' width='425'>Product Link</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>DE-Cadherin</td><td class='xl64' style='border-top: none; border-left: none;'>rat</td><td class='xl64' style='border-top: none; border-left: none;'>DCAD2</td><td class='xl65' style='border-top: none; border-left: none;'>1:16</td><td class='xl67' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/cadherin-DE-</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Coracle</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>50:50 mix of C566.9 and C615.16</td><td class='xl65' style='border-top: none; border-left: none;'>1:50</td><td class='xl66' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/Coracle and http://dshb.biology.uiowa.edu/Coracle_2</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Engrailed&nbsp;</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>4D9</td><td class='xl65' style='border-top: none; border-left: none;'>1:10</td><td class='xl67' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/4D9-anti-engrailed-invected</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Alpha-spectrin</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>3A9</td><td class='xl65' style='border-top: none; border-left: none;'>1:16</td><td class='xl67' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/3A9-323-or-M10-2_2</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Mmp</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>&nbsp;3A6B4</td><td class='xl65' style='border-top: none; border-left: none;'>1:20</td><td class='xl67' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/3A6B4</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Hindsight</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>1G9</td><td class='xl65' style='border-top: none; border-left: none;'>1:20</td><td class='xl67' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/hindsight-protein</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Atp-alpha</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>a5</td><td class='xl65' style='border-top: none; border-left: none;'>1:50</td><td class='xl67' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/a5</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Nervana</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>Nrv5F7</td><td class='xl65' style='border-top: none; border-left: none;'>1:50</td><td class='xl67' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/Nervana-protein</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Discs Large</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>4F3</td><td class='xl65' style='border-top: none; border-left: none;'>1:10</td><td class='xl67' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/4F3-anti-discs-large</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Fasciclin III</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>7G10</td><td class='xl65' style='border-top: none; border-left: none;'>1:100</td><td class='xl68' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/7G10-anti-Fasciclin-III_2</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Crumbs (concentrate)</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>Cq4-c</td><td class='xl65' style='border-top: none; border-left: none;'>1:50</td><td class='xl68' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/Crumbs</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Armadillo</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>N2 7A1</td><td class='xl65' style='border-top: none; border-left: none;'>1:50</td><td class='xl68' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/N2-7A1-ARMADILLO</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Neuroglian</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>BP 104</td><td class='xl65' style='border-top: none; border-left: none;'>1:25</td><td class='xl68' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/neuroglian</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Flamingo</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>Flamingo #74</td><td class='xl65' style='border-top: none; border-left: none;'>1:20</td><td class='xl68' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/Flamingo-74</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>N-Cadherin</td><td class='xl64' style='border-top: none; border-left: none;'>rat</td><td class='xl64' style='border-top: none; border-left: none;'>DN-Ex #8</td><td class='xl65' style='border-top: none; border-left: none;'>1:20</td><td class='xl68' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/cadherin-DN-</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Rho1</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>p1D9</td><td class='xl65' style='border-top: none; border-left: none;'>1:40</td><td class='xl68' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/p1D9-anti-rho1</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Alpha-catenin</td><td class='xl64' style='border-top: none; border-left: none;'>rat</td><td class='xl64' style='border-top: none; border-left: none;'>DCAT-1</td><td class='xl65' style='border-top: none; border-left: none;'>1:10</td><td class='xl68' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/catenin-alpha-</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Draper</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>Draper 8A1</td><td class='xl65' style='border-top: none; border-left: none;'>1:25</td><td class='xl68' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/Draper8A1</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Delta</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>C594.9B</td><td class='xl65' style='border-top: none; border-left: none;'>1:50</td><td class='xl68' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/Delta-extracellular-domain</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Elav</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>ELAV-9F8A9</td><td class='xl65' style='border-top: none; border-left: none;'>1:100</td><td class='xl68' style='border-top: none; border-left: none;'>http://dshb.biology.uiowa.edu/Elav-9F8A9</td></tr></tbody></table><br />Custom Antibodies<br /><table style='border-collapse: collapse; width: 410pt;' border='0' width='410' cellspacing='0' cellpadding='0'><tbody><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; width: 90pt;' width='90' height='15'>Antigen</td><td class='xl64' style='border-left: none; width: 69pt;' width='69'>Host Species</td><td class='xl64' style='border-left: none; width: 65pt;' width='65'>Product ID</td><td class='xl65' style='border-left: none; width: 77pt;' width='77'>Concentration</td><td class='xl64' style='border-left: none; width: 109pt;' width='109'>Citation - Pubmed ID</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Pio-Pio</td><td class='xl64' style='border-top: none; border-left: none;'>rabbit</td><td class='xl64' style='border-top: none; border-left: none;'>Custom</td><td class='xl65' style='border-top: none; border-left: none;'>1:100</td><td class='xl64' style='border-top: none; border-left: none;' align='right'>12973360</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Neuroglian (1B7)</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>Custom</td><td class='xl65' style='border-top: none; border-left: none;'>1:10</td><td class='xl64' style='border-top: none; border-left: none;'>2805067&nbsp;</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>sqh-1P</td><td class='xl64' style='border-top: none; border-left: none;'>guinea pig</td><td class='xl64' style='border-top: none; border-left: none;'>Custom</td><td class='xl65' style='border-top: none; border-left: none;'>1:250</td><td class='xl64' style='border-top: none; border-left: none;' align='right'>20920606</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>sqh-2P</td><td class='xl64' style='border-top: none; border-left: none;'>rat&nbsp;</td><td class='xl64' style='border-top: none; border-left: none;'>Custom</td><td class='xl65' style='border-top: none; border-left: none;'>1:500</td><td class='xl64' style='border-top: none; border-left: none;' align='right'>20920606</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Mcr</td><td class='xl64' style='border-top: none; border-left: none;'>guinea pig</td><td class='xl64' style='border-top: none; border-left: none;'>Custom</td><td class='xl65' style='border-top: none; border-left: none;'>1:400</td><td class='xl64' style='border-top: none; border-left: none;' align='right'>24496625</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Contactin</td><td class='xl64' style='border-top: none; border-left: none;'>guinea pig</td><td class='xl64' style='border-top: none; border-left: none;'>Custom</td><td class='xl65' style='border-top: none; border-left: none;'>1:2000</td><td class='xl64' style='border-top: none; border-left: none;' align='right'>15459097</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Kune</td><td class='xl64' style='border-top: none; border-left: none;'>rabbit</td><td class='xl64' style='border-top: none; border-left: none;'>Custom</td><td class='xl65' style='border-top: none; border-left: none;'>1:1000</td><td class='xl64' style='border-top: none; border-left: none;' align='right'>20407131</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Neurexin</td><td class='xl64' style='border-top: none; border-left: none;'>rabbit</td><td class='xl64' style='border-top: none; border-left: none;'>Custom</td><td class='xl65' style='border-top: none; border-left: none;'>1:500</td><td class='xl64' style='border-top: none; border-left: none;'>8978610</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Uninflatable</td><td class='xl64' style='border-top: none; border-left: none;'>guinea pig</td><td class='xl64' style='border-top: none; border-left: none;'>Custom</td><td class='xl65' style='border-top: none; border-left: none;'>1:400</td><td class='xl64' style='border-top: none; border-left: none;' align='right'>19818339</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Mtf</td><td class='xl64' style='border-top: none; border-left: none;'>guinea pig</td><td class='xl64' style='border-top: none; border-left: none;'>Custom</td><td class='xl65' style='border-top: none; border-left: none;'>1:500</td><td class='xl64' style='border-top: none; border-left: none;' align='right'>20935638</td></tr><tr style='height: 15.0pt;'><td class='xl64' style='height: 15.0pt; border-top: none;' height='15'>Squash</td><td class='xl64' style='border-top: none; border-left: none;'>mouse</td><td class='xl64' style='border-top: none; border-left: none;'>Custom</td><td class='xl65' style='border-top: none; border-left: none;'>1:400</td><td class='xl64' style='border-top: none; border-left: none;' align='right'>20920606</td></tr></tbody></table>

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