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<p><strong>Lysogeny <a href="https://en.wikipedia.org/wiki/Broth" target="_blank">broth</a></strong> (<strong>LB</strong>), a <a href="https://en.wikipedia.org/wiki/Nutrient" target="_blank">nutritionally</a> rich <a href="https://en.wikipedia.org/wiki/Growth_medium" target="_blank">medium</a>, is primarily used for the <a href="https://en.wikipedia.org/wiki/Bacterial_growth" target="_blank">growth</a> of <a href="https://en.wikipedia.org/wiki/Bacterium" target="_blank">bacteria</a>. The initialism is also commonly, albeit incorrectly, taken to mean <strong>Luria broth</strong>, <strong>Lennox broth</strong>, or <strong>Luria-Bertani medium</strong>. According to its creator <a href="https://en.wikipedia.org/w/index.php?title=Giuseppe_Bertani&amp;action=edit&amp;redlink=1" target="_blank">Giuseppe Bertani</a>, the abbreviation <strong>LB</strong> was actually intended to stand for <strong>lysogeny broth</strong>.<sup class="reference"><a href="https://en.wikipedia.org/wiki/Lysogeny_broth#cite_note-B04-1" target="_blank">[1]</a></sup> The formula of the <strong>LB medium</strong> was published in 1951 in the first paper of Bertani on <a href="https://en.wikipedia.org/wiki/Lysogeny" target="_blank">lysogeny</a>. In this article he described the modified <a href="https://en.wikipedia.org/w/index.php?title=Single-burst_experiment&amp;action=edit&amp;redlink=1" target="_blank">single-burst experiment</a> and the isolation of the phages <a href="https://en.wikipedia.org/wiki/Enterobacteria_phage_P1" target="_blank">P1</a>, <a href="https://en.wikipedia.org/wiki/Enterobacteria_phage_P2" target="_blank">P2</a>, and <a href="https://en.wikipedia.org/w/index.php?title=Enterobacteria_phage_P3&amp;action=edit&amp;redlink=1" target="_blank">P3</a>. He had developed the <strong>LB medium</strong> to optimize <em><a href="https://en.wikipedia.org/wiki/Shigella" target="_blank">Shigella</a></em> growth and <a href="https://en.wikipedia.org/wiki/Virus_Quantification#Plaque_assay" target="_blank">plaque formation</a>.<sup class="reference"><a href="https://en.wikipedia.org/wiki/Lysogeny_broth#cite_note-B04-1" target="_blank">[1]</a></sup><sup class="reference"><a href="https://en.wikipedia.org/wiki/Lysogeny_broth#cite_note-2" target="_blank">[2]</a></sup></p>
<p>LB media formulations have been an industry standard for the cultivation of <em><a href="https://en.wikipedia.org/wiki/Escherichia_coli_(molecular_biology)" target="_blank">Escherichia coli</a></em> as far back as the 1950s.<sup class="reference"><a href="https://en.wikipedia.org/wiki/Lysogeny_broth#cite_note-M72-3" target="_blank">[3]</a></sup><sup class="reference"><a href="https://en.wikipedia.org/wiki/Lysogeny_broth#cite_note-L60-4" target="_blank">[4]</a></sup><sup class="reference"><a href="https://en.wikipedia.org/wiki/Lysogeny_broth#cite_note-L55-5" target="_blank">[5]</a></sup><sup class="reference"><a href="https://en.wikipedia.org/wiki/Lysogeny_broth#cite_note-L57-6" target="_blank">[6]</a></sup><sup class="reference"><a href="https://en.wikipedia.org/wiki/Lysogeny_broth#cite_note-A46-7" target="_blank">[7]</a></sup> These media have been widely used in <a href="https://en.wikipedia.org/wiki/Molecular_microbiology" target="_blank">molecular microbiology</a>applications for the preparation of <a href="https://en.wikipedia.org/wiki/Plasmid" target="_blank">plasmid</a> <a href="https://en.wikipedia.org/wiki/DNA" target="_blank">DNA</a> and <a href="https://en.wikipedia.org/wiki/Recombinant_DNA" target="_blank">recombinant</a> proteins. It continues to be one of the most common media used for maintaining and cultivating laboratory recombinant strains of<em>Escherichia coli</em>.<sup class="reference"><a href="https://en.wikipedia.org/wiki/Lysogeny_broth#cite_note-sambrook-8" target="_blank">[8]</a></sup> For physiological studies however, the use of LB medium is to be discouraged.<sup class="reference"><a href="https://en.wikipedia.org/wiki/Lysogeny_broth#cite_note-9" target="_blank">[9]</a></sup></p>
<p>There are several common formulations of LB. Although they are different, they generally share a somewhat similar composition of ingredients used to promote growth, including the following:</p>
<ul>
<li><a href="https://en.wikipedia.org/wiki/Peptides" target="_blank">Peptides</a> and <a href="https://en.wikipedia.org/wiki/Casein" target="_blank">casein</a> <a href="https://en.wikipedia.org/wiki/Peptide" target="_blank">peptones</a></li>
<li><a href="https://en.wikipedia.org/wiki/Vitamins" target="_blank">Vitamins</a> (including B vitamins)</li>
<li>Trace elements (e.g. nitrogen, sulfur, magnesium)</li>
<li>Minerals</li>
</ul>
<p>Peptides and peptones are provided by <a href="https://en.wikipedia.org/wiki/Tryptone" target="_blank">tryptone</a>. Vitamins and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by <a href="https://en.wikipedia.org/wiki/Sodium_chloride" target="_blank">sodium chloride</a>. Tryptone is used to provide essential <a href="https://en.wikipedia.org/wiki/Amino_acid" target="_blank">amino acids</a> to the growing bacteria, while the <a href="https://en.wikipedia.org/wiki/Yeast_extract" target="_blank">yeast extract</a> is used to provide a plethora of <a href="https://en.wikipedia.org/wiki/Organic_compound" target="_blank">organic compounds</a> helpful for bacterial growth.</p>
<p>In his original 1951 paper, Bertani used 10 grams of NaCl and 1 gram of glucose per 1 L of solution; Luria in his 'L broth' of 1957 copied Bertani's original recipe exactly.<sup class="reference"><a href="https://en.wikipedia.org/wiki/Lysogeny_broth#cite_note-L57-6" target="_blank">[6]</a></sup> Recipes published later have typically left out the glucose.</p>
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<p><strong>From Wikipeida </strong><strong>https://en.wikipedia.org/wiki/Lysogeny_broth</strong></p>

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