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[DESCRIPTION]
<p>SWIFT™ Western Transfer Pads For Efficient High &amp; Low MW Protein Transfer</p>
<p>(Cat. # <a href="http://www.gbiosciences.com/ResearchProducts/swift-desc.aspx" target="_blank">786‐370 to 786‐375, 786‐370S, 786‐373S</a>)</p>

[BEFORE_START]
<p>Combine 50ml of supplied SWIFT™ Western Buffer with 850ml deionized water and 100ml Methanol to give 1L final volume.  For best results, we recommend to chill the diluted SWIFT™ Western Buffer at 4° C before use.</p>
<p> </p>
<p><strong>NOTE</strong>: The buffer volume preparation can be adjusted as per blotting unit size.  </p>

[GUIDELINES]
<p><strong>INTRODUCTION</strong></p>
<p> </p>
<p>Western blot analysis of proteins is a routine and commonly used technique in most research laboratories, with 3 major drawbacks.  The first is the variable efficiency of the transfer; the second is the problem of transferring high molecular weight proteins; and the third is the amount of time taken to transfer the proteins to a binding membrane. Other minor drawbacks also exist with the Western blotting technique and these include overheating of the apparatus, shorting out of power packs due to excess current and the messy assembling of transfer sandwiches.</p>
<p> </p>
<p>SWIFT™ Western Transfer pads alleviate the above issues encountered with Western blotting and produces consistent and efficient transfer of high and low molecular weight proteins, while reducing the transfer time by approximately 50%.  The SWIFT™ Western Transfer pads Cat. #   786‐370prevent overheating and power shortages by maintaining the lower current in the transfer buffer , without affecting transfer efficiency.   </p>
<p> </p>
<p> </p>
<p><strong>ITEM(S) SUPPLIED</strong></p>
<p> </p>
<table>
<tbody>
<tr>
<td><strong>Pad/ Membrane Size 8.5 x 7.5cm</strong></td>
<td><strong>Cat. #   786‐370</strong></td>
<td><strong>Cat. # 786‐371</strong></td>
<td><strong>Cat. # 786‐372</strong></td>
<td><strong>Cat. # 786‐370S</strong></td>
</tr>
<tr>
<td>SWIFT™ Western Buffer</td>
<td> 2 x 250ml</td>
<td>2 x 250ml</td>
<td>2 x 250ml</td>
<td>1 x 100ml</td>
</tr>
<tr>
<td>SWIFT™ Western Transfer Pads</td>
<td> 2 x 10</td>
<td> 2 x 10</td>
<td> 2 x 10</td>
<td> 4 </td>
</tr>
<tr>
<td>SWIFT™ Template Card</td>
<td> 1</td>
<td> 1</td>
<td> 1</td>
<td> 1</td>
</tr>
<tr>
<td>PVDF Membrane</td>
<td> N/A</td>
<td> N/A</td>
<td> 10</td>
<td> N/A</td>
</tr>
<tr>
<td>Nitrocellulose Membrane</td>
<td> N/A</td>
<td> 10</td>
<td> N/A</td>
<td> N/A</td>
</tr>
<tr>
<td><strong>Pad/ Membrane Size 9.5 x 15cm </strong></td>
<td><strong>Cat. # 786‐373 </strong></td>
<td><strong>Cat. # 786‐374</strong></td>
<td><strong>Cat. # 786‐375</strong></td>
<td><strong>Cat. # 786‐373S</strong></td>
</tr>
<tr>
<td>SWIFT™ Western Buffer</td>
<td> 2 x 250ml</td>
<td> 2 x 250ml</td>
<td> 2 x 250ml</td>
<td> 1 x 100ml</td>
</tr>
<tr>
<td>SWIFT™ Western Transfer Pads</td>
<td> 10</td>
<td> 10</td>
<td> 10</td>
<td> 4</td>
</tr>
<tr>
<td>SWIFT™ Template Card</td>
<td> 1</td>
<td> 1</td>
<td> 1</td>
<td> 1</td>
</tr>
<tr>
<td>PVDF Membrane</td>
<td> N/A</td>
<td> N/A</td>
<td> 5</td>
<td> N/A</td>
</tr>
<tr>
<td>Nitrocellulose Membrane</td>
<td> N/A</td>
<td> 5</td>
<td> N/A</td>
<td> N/A</td>
</tr>
</tbody>
</table>
<p> </p>
<p><strong>IMPORTANT INFORMATION</strong></p>
<p> </p>
<p>Keep the SWIFT™ Western Transfer Pads bag sealed after use to avoid drying of the pads. If pads dry out, add diluted SWIFT™ Western Buffer to wet before use.</p>
<p> </p>
<p><strong>STORAGE CONDITIONS</strong></p>
<p> </p>
<p>The kit is shipped at ambient temperature. Upon arrival, store at room temp and is stable for 1year.  </p>
<p> </p>
<p><strong> PREPARATION BEFORE USE</strong> </p>
<p> </p>
<p>Combine 50ml of supplied SWIFT™ Western Buffer with 850ml deionized water and 100ml Methanol to give 1L final volume.  For best results, we recommend to chill the diluted SWIFT™ Western Buffer at 4° C before use.</p>
<p> </p>
<p><strong>NOTE</strong>: The buffer volume preparation can be adjusted as per blotting unit size.  </p>
<p> </p>
<p> </p>

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