eamag/protocols-with-steps huggingface dataset:
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This method describes magnetic particle based DNA purification.  </p>
<p> </p>
<p> </p>
<p> </p>

[BEFORE_START]
<p>Make 80% Ethanol.  Do not use 80% Ethanol that is more than 3 days old. </p>

[GUIDELINES]
<p><strong>Materials:</strong></p>
<p> </p>
<p>80% Ethanol, made fresh</p>
<p>'beads' - <a href="https://protocols.io/view/1-5-mg-ml-sera-mag-carbolylate-modified-magnetic-p-g2abyae" target="_blank">1.5 mg/mL Sera-mag magnetic particles</a>  or  Agencourt AMPure XP beads, Beckman Coulter A63880/1/2</p>
<p>DNA sample needing purification and/or concentrating</p>
<p>10 mM Tris-HCl (pH 8.0 - 8.5)</p>
<p> </p>
<p> </p>
<p> </p>
<p><strong>Equipment:</strong>  </p>
<p> </p>
<p>Stand or plate magnet compatible with sample size such as:</p>
<p>    DynaMag-2 from Thermo-Fischer, 12321D</p>
<p>    http://www.eandkscientific.com/Magnet-Plates/</p>
<p> </p>
<p> </p>

[STEPS]
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TITLE: No Title
AUTHORS: 

[BEFORE_START]
<p>The protocol remains unmodified from original kit protocol, with the exception of steps 9, 10 and 11.</p>

[STEPS]
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TITLE: Virome Extraction
AUTHORS: Frej   Larsen

[DESCRIPTION]
This protocol is for isolation of bacteriophages from fecal matter. It is based on the protocol used for the COPSAC10 cohort and was originally described by Ling Deng in doi:10.3390/v11070667. Extraction is performed using centrifugation and ultrafiltration to isolate viral particles and get rid of contaminants.

[BEFORE_START]
Fill bucket with crushed ice

Turn on centrifuge and start the fast temp program to reach 4◦ C

Cool SM buffer in ice bucket

[GUIDELINES]
This protocol is designed for extraction of bacteriophages from fecal samples. The sample should be 50-500mg of fecal material diluted in buffer (2xSM buffer for instance). Sample sizes outside these limits have not been tested and correct sequencing results can not be guaranteed.

[STEPS]
1. Thaw frozen sample in ice bucket
2. Transfer up to 500 mg of sample to centrifuge tube and add  SM buffer to reach a final volume of 5 mL. Place centrifuge tube back in ice box

3. Homogenize samples by placing ice box on shaking board at   

4. Centrifuge samples at5000 rcf, 30 min, 4 °C 

5. Filter the supernatant  through a 0.45 um PES filter into a new centrifuge tube
6. Transfer 2.5 mL of the sample to the outer chamber of the concentrator

7. Centrifuge sample in the concentrator at 1500 rcf, 20 min, 4 °C 

8. Pour off liquid from the inner chamber
9. Repeat the three previous steps until all liquid from the centrifuge tube has passed through the concentrator and the there is no liquid left in the inner chamber
10. Add 100 ul SM buffer to the inner chamber and centrifuge 1500 rcf, 2 min, 4 °C 

11. Cut off filter from bottom of inner chamber using a sterile scalpel and transfer to new eppendorf tube. Transfer remaining liquid from outer chamber to the eppendofr tube
12. Leave in fridge at least 12 hours and at most for 1 week before proceeding with spotting or DNA/RNA extraction
TITLE: S1File_FullQuestionnaire
AUTHORS: Chris Englert, Dennis Koroma, Alex Bertrams, Corinna Martarelli

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>The attention control video has been frequently applied to test the ego depletion effect. However, its validity has never been tested, a shortcoming we address in this preregistered study. In the first task, self-control strength was temporarily depleted in the depletion condition (</span><span style = "font-style:italic;">n</span><span> = 56) but remained intact in the control condition (</span><span style = "font-style:italic;">n</span><span> = 56). The attention control video served as the secondary task, and we assumed that the depletion condition would perform significantly worse compared to the control condition. Attention regulation was measured with an eye-tracking device. The results revealed that the gaze behavior in the two conditions differed statistically significantly; however, the actual difference was small, indicating that the attention control video may not be an optimal measure of self-control.</span></div></div>

[STEPS]
TITLE: U Cinn - Non-invasive Measurement of Intestinal Fat Absorption
AUTHORS: Patrick Tso, Dana Lee

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary:  </span></div><div class = "text-block">Dietary fat containing 5% sucrose polybehenate, a non-absorbable food additive, is fed in a semi-synthetic diet containing absorbable fat to rodents. The animals eat the diet ad lib for 3 days. Two or more fecal pellets are collected on days 3 and 4. Fat absorption is calculated from the ratios of behenic acid to other fatty acids in the diet and in the feces as analyzed by gas chromatography of fatty acid methyl esters.</div></div>

[STEPS]
?. Animal Feeding and Fecal Collection: MONDAY:                     REPLACE CHOW WITH TEST DIET TUESDAY:                    CONTINUE TEST DIET,                                           NEW CAGE BEDDING BEFORE FEEDING PERIOD WEDNESDAY:              CONTINUE TEST DIET(DAY 3 – RETURN)                                          COLLECT 5-8 FECAL PELLETS/ANIMAL                                          CHANGE CAGE BEDDING BEFORE FEEDING PERIOD THURSDAY:                  COLLECT 5-8 FECAL PELLETS/ANIMAL (DAY 4 – RETURN)
TITLE: CARD COUNT PROTOCOL
AUTHORS: Grace Sandel

[DESCRIPTION]
Procedure to conduct the card count portion of "Crazy Yellow Ant Sampling on Tetiaroa"
The point of this protocol is to quantify the prevalence of yellow crazy ants in given transects around Tetiaroa. 

[BEFORE_START]
Follow this protocol after arriving at the sample site coordinates (ex: D5, E17). 
Do card counts before deploying vials. 

[GUIDELINES]
- Do not trespass
- Analyze site safety before conducting a sample
- Avoid trampling flora 
- If a point is inside a building, just go to the next point or as close to the point as possible

[STEPS]
SECTION: Card Count Protocol
1. If needed, scrape the ground (at least a 10x10cm area) at the site so the card sits flat on the ground (i.e. if there are branches or leaf litter, scrape them away).
SECTION: Card Count Protocol
3. Place the card down on the ground.
SECTION: Card Count Protocol
4. For 30 seconds, count the number of yellow crazy ants that cross the card.
SECTION: Card Count Protocol
5. After 30 seconds have passed, pick up the card and remove all ants from it. 
SECTION: Card Count Protocol
2. If you scraped the ground, wait 30 seconds before placing the card down. If you did not scrape the ground, place the card down without waiting.
SECTION: Card Count Protocol
6. Enter the number of ants counted in the card count into the appropriate field of the EpiCollect5 form.
TITLE: WIPI2d construct cloning
AUTHORS: lmstrong 

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">WIPI2d cloning </div></div>

[STEPS]
?. Amplify gene using PCR with Q5 polymerase. Design primers to be cut with ClaI and XmaI. For mutants, design overlapping primers in opposing direction as if using around the horn. Use these to perform 2-step PCR, verifying each step via gel.
?. Gel extract correct size band and measure concentration
?. Digest insert with ClaI and XmaI in Cutsmart buffer at 37C for 1 hour. Digest pCAG vector with same enzymes. After 1 hour add CIAP to vector digest and incubate at 37 for another 30 minutes
?. Gel extract vector digest. PCR clean up insert digest. Measure concentrations of both.
?. Mix vector and insert at 1:4 molar ratio for ligation. Add 1uL T4 ligase and 2uL T4 ligase buffer in 20uL reaction. Incubate at RT for 10 minutes.
?. Transform 4uL of reaction into DH5alpha cells. Plate everything. Sequence colonies to ensure correct mutation.
TITLE: Total Starch Enzymatic Digestion 
AUTHORS: Lynn   Doran, Amanda P. De Souza

[DESCRIPTION]
Enzymatic digestion of total soluble starch to glucose in plant tissue extracts for preparation for quantification via the GOD-POD Method (NZYtech).

[BEFORE_START]
Extract and dry total starch pellet from plant tissue per Extraction of Non-Structural Carbohydrates (Total Soluble Sugars + Starch) in Plant Tissues.

[STEPS]
1. Prepare fresh daily 120 U/mL α-amylase in MOPS buffer. 1 mL per sample will be needed. Initial concentration of α-amylase is 1000 U/mL.  Use C1V1 = C2V2 to calculate the volume of α-amylase and MOPS buffer to use.
 

2. Prepare fresh daily 30 U/mL amyloglucosidase in acetate buffer. 1 mL per sample will be needed. Initial concentration of amyloglucosidase is 3260 U/mL.  Use C1V1 = C2V2 to calculate the volume of amyloglucosidase  and acetate buffer to use.
 

3. Heat the water bath to 75 °C.

4. Add 500 µL of α-amylase in MOPS buffer (120 U/mL) to each sample tube.

5. Vortex to suspend all solids.  Flicking the tube may help dislodge the dried starch pellet.
6. Incubate for 30 min at 75 °C in the water bath.

7. Add another 500 µL of α-amylase in MOPS buffer (120 U/mL) to each sample tube.
8. Vortex to suspend all solids.
9. Incubate for 30 min at 75 °C in the water bath.

10. Cool down the water bath to 50 °C, tubes can be stored at room temp on the counter while water bath cools.
 

11. Add 500 µL amyloglucosidase in acetate buffer (30 U/mL) to each sample tube.

12. Vortex to suspend all solids.
13. Incubate for 30 min at 50 °C in the water bath.

14. Add another 500 µL amyloglucosidase in acetate buffer (30 U/mL) to each sample tube.

15. Vortex to suspend all solids.
17. Place the tubes in ice to stop the reaction, until cool to the touch.
18. Proceed to total starch (as glucose) quantification by NZYtech GOD-POD method or store the samples at -20°C up to one month.
16. Incubate for 30 min at 50 °C in the water bath.
TITLE: No Title
AUTHORS: 

[STEPS]
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TITLE: Wide-field imaging of voltage sensors expressed in ex vivo mouse brain slices
AUTHORS: Yan-Feng Zhang, Stephanie J Cragg

[DESCRIPTION]
This protocol describes how to perform wide-field imaging of voltage sensors using high frame rates (660 Hz minimum every 2.5 minutes) in mouse midbrain using ex vivo brain slices. 

[BEFORE_START]
We injected the voltage sensor ASAP3 without a soma-targeting signal (AAV5-EF1α-DIO-ASAP3WPRE) following the steps described in Protocol: Intracranial injections of viral vectors in mouse midbrain and striatum. We injected the virus diluted to 2.4E+12 vg/ml in the midbrain (1 μL per site) of heterozygous DAT-IRES-Cre mice.

The coordinates used by us for targeting the midbrain were as follows:

Ventral tegmental area (VTA) (AP = -3.1 mm, ML = ± 0.5 mm, DV = -4.4 mm)
Substancia nigra pars compacta (SNc) (AP = -3.5 mm, ML = ± 1.2 mm , DV = -4.0 mm)

Animals were maintained for at least three weeks following surgery to allow virus expression in the midbrain. We then prepare ex vivo brain slices by performing steps 1 to 11 from Protocol: Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices. 

[STEPS]
SECTION: Image Acquisition
1. Using a x40/0.8 NA water-objective (Olympus UK), position the stimulating electrode on the surface of the brain slice and centre it in the field of view.
SECTION: Image Analysis
5. The following steps were performed in MATLAB vR2019b and Fiji v1.5.

Extract ﬂuorescence intensity from the region of interest (~5 µm * 5 µm).
SECTION: Image Acquisition
3. Apply electrical stimulus pulses singly and in trains (4 pulses, 50 Hz) using PClamp.


 

SECTION: Image Analysis
6. Bleach-correct the ASAP3 transients by ﬁtting an exponential curve function.
SECTION: Image Analysis
7. Expressed data as ΔF/F where F is the ﬁtted curve.
SECTION: Image Acquisition
2. Change the exposure time to reach a frame rate of around 600 Hz every 2.5 min using Micro-Manager v1.4.
SECTION: Image Acquisition
4. Record changes in fluorescence intensity using PClamp.
TITLE: Swarming assay (high number)
AUTHORS: Serena Ding

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">For imaging swarming behaviour of a very high number (thousands+) of young adult C. elegans on agar using the Cyclops single worm tracker. Worms are synchronised by bleaching and refeeding for 72 hours, and then 300 uL of young adult hermaphrodites are transferred by glass pipette onto a 90 mm regular NGM plate for imaging for 15 hours at 0.1Hz.</div></div>

[STEPS]
?. [Bleach synchronising worms (-7 to -4 day)]
Bleach synchronise 3 large plates (90mm) worth of animals, making sure lots of gravid hermaphrodites are present. Leave on rotator at 20 °C until use.
?. [Imaging (Day 0 PM)]
Place the plate inside the tracker with an appropriate field of view (i.e. worm front at the edge of the illuminated circular field of field, so that they are expected to sweep over the field of view). Image overnight.
?. [Imaging (Day 1 AM)]
Close Gecko. Transfer the files to the server.
?. [Imaging (Day 0 PM)]
Transfer ~160 uL of worm onto an imaging plate (90 mm plate pre-seeded with undiluted OP50 at least one day prior) using a glass pipette, making sure to carry as little liquid as possible as this will disperse bacteria on the imaging plate.
Some liquid will inevitably spill onto the lawn, which is ok. This is where the worm aggregate/front initially forms.
?. [Imaging (Day 0 PM)]
Set appropriate frame rate using CommonVision control box (0.1 Hz). Open Gecko and initialise experiments: fixed duration (60 minute recording, repeat 15 times), MJPG format.
?. [Re-feeding worms (-3 day PM)]
Re-feed worms onto 3-4 large plates (90mm) using a glass pipette. Incubate at 20 °C.
90 mm plates are pre-seeded with undiluted OP50. Lawn does not have to be any specific shape - uniform, with gaps, all is ok, depending on experimental needs.
?. [Imaging (Day 0 PM)]
Pool animals together from 3 large plates (90mm), wash in M9 twice, and aspire as much supernatent as possible after the last wash.
TITLE: Nanopore amplicon sequencing with DIY adapter
AUTHORS: Jie Hao Ou, Yin-Tse Huang

[DESCRIPTION]
Nanopore amplicon sequencing with DIY adapter

[STEPS]
SECTION: Workflow
1.    

SECTION: Preparation of amplicons
2. Use a primer with a HEAD to make amplicons, as in a regular procedure. 
Enzymes with or without proofreading can be used, and purification is not necessary.
SECTION: Pooling &amp; 5&#x27; Phosphorylation
5. 
Mix the following materials in order:
(10 samples as an example)
 
#MaterialQuantity1Sterile water7 ul2T4 Polynucleotide Kinase Reaction Buffer2 ul3T4 Polynucleotide Kinase1 ul4PCR product (x10 samples)10 ul
 
 
SECTION: Preparation of Adapter
9. Order the following two primers:
Adapter_top: TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT
Adapter_bottom: GCAATACGTAACTGAACGAAGTACAGG
Preferably, choose OPC or a higher level of purification method.
SECTION: Preparation of Adapter
10. Dilute both primers to 100uM according to the manufacturer's instructions 
(TE buffer is recommended)
SECTION: Preparation of Adapter
11. Mix both primers together in equal volumes.
(Now it is 50 uM)
SECTION: Preparation of Adapter
12. Incubate the mixture in a dry bath at 95°C for 2 minutes.


  

SECTION: Preparation of Adapter
13. Allow the mixture to slowly cool to room temperature for one hour.
SECTION: Preparation of Adapter
14. Make 1:10 dilution (5 uM)
Store the mixture at 4°C or in a freezer for long-term storage. 
When necessary, divide the mixture into smaller portions to avoid repeated thawing.
4. Purify the PCR product and adjust the final concentration of the purified DNA to ~50 ng/ul.
3. Use a barcoding index for the second PCR. 
Note: 
1. non-proofreading enzyme (Taq) must be used to add a A tail to the PCR product.
SECTION: Pooling &amp; 5&#x27; Phosphorylation
6. Incubate at 37°C for 30 minutes.
SECTION: Pooling &amp; 5&#x27; Phosphorylation
7. Heat inactivate by incubating at 65°C for 20 minutes
SECTION: Pooling &amp; 5&#x27; Phosphorylation
8. Purify the DNA and adjust the final concentration to ~50 ng/ul.
SECTION: Attach adapter to PCR products.
15. Mix the following materials in order:

 

SECTION: Attach adapter to PCR products.
17. Purify the DNA and adjust the final concentration to ~50 ng/ul.
SECTION: Attach adapter to PCR products.
16. Incubate at  16-20 °C  for at least 60 min
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This protocol is aimed at amplifying the 16S rRNA hypervariable region 4 (16S V4) in prokaryotes. The primers (515fB and 806rB, <span style="font-weight: 400;">926R</span>) used in this protocol are based on the primer utilized in Apprill et al 2015 and the Earth Microbiome Project (EMP).</p>
<p> </p>
<p>This work was supported by NASA grant NNX14AP62A ‘National Marine Sanctuaries as Sentinel Sites for a Demonstration Marine Biodiversity Observation Network (MBON)’ funded under the National Ocean Partnership Program (NOPP RFP NOAA-NOS-IOOS-2014-2003803 in partnership between NOAA, BOEM, and NASA), and the U.S. Integrated Ocean Observing System (IOOS) Program Office.</p>
<p> </p>
<p>Citation<br />Apprill, A., McNally, S., Parsons, R. &amp; Weber, L. Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. Aquat Microb Ecol 75, 129–137 (2015). <a href="http://doi.org/10.3354/ame01753" target="_blank" rel="noopener noreferrer">doi:10.3354/ame01753</a></p>
<p> </p>
<p>Earth Microbiom Project<br />http://press.igsb.anl.gov/earthmicrobiome/protocols-and-standards/16s/</p>

[BEFORE_START]
<p>Disinfect work surfaces with 10% bleach, followed by 70% ethanol, then RNase Away and pipets with RNase Away. UV pipets, molecular grade water, and tube racks for 20 minutes prior to starting protocol.</p>

[GUIDELINES]
<div>
<div>
<div>
<p>Local containment involves the employment of biological <em>safety</em> cabinets for initial specimen disinfection and <em>DNA</em> extraction. Such cabinets should not be used for <em>PCR</em> setup, as this <em>procedure</em> should be conducted apart from the aforementioned disinfection and extraction to minimize contamination.</p>
<p> </p>
<ol>
<li>Do not use any tube or plate that is not appropriate for the PCR machine you are using.</li>
<li>Make sure tubes and especially plates are well sealed before you begin run.</li>
<li>Clean up any spilled solutions and dispose of in appropriate biohazard boxes.</li>
<li>Be careful with PCR machine lids. These can be damaged if you slam or drop lids.</li>
<li>Make sure PCR heater block is clean before you start a run. Check each tube receptacle before you start.</li>
<li>Distribute tubes evenly across block so lid will seat flat against top of tubes for even heating and sealing.</li>
<li>Turn PCR machine off when you are done using.</li>
</ol>
</div>
</div>
</div>

[STEPS]
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TITLE: Isolation of intestinal organoids from matrigel for protein or RNA extraction
AUTHORS: rachel.bates

[DESCRIPTION]
Isolation of intestinal organoids from matrigel or cultrex growth domes ready for protein or RNA isolation. 

[STEPS]
1. Intestinal organoids are grown in 30 µL     domes for 14400 min 

2. Once organoids are large enough for harvest domes are washed three times with ice cold   .

3. Add  1 mL per well. incubate on ice for 60 min with orbital shaking at   . 

4. Harvest well contents into centrifuge tube. Centrifuge 6000 rpm, 1 min . Carefully remove supernatant and add 1 mL of 4 °C PBS. Incubate on ice for 5 min . 

5. Centrifuge 6000 rpm, 1 min, 4 °C . Carefully remove the supernatant and as much solidified matrigel as possible leaving behind organoids. Cell pellet is ready for RNA or protein extraction.
TITLE: Qubit
AUTHORS: Dakota Betz

[DESCRIPTION]
Our protocols are constantly evolving and old versions will be deleted.
The documents here are not intended to be cited in publications

[STEPS]
SECTION: Setup
2. Take out standards (1 and 2) and Qubit buffer from cabinet above NGS bench. Also get the Qubit reagent from the labled blue box in the drawer under the NGS bench. Retrieve DNA extractions and defrost (if they were frozen previously). Be sure to vortex and centrifuge samples. 
SECTION: Setup
3. Clean the NGS area well, including the Qubit machine, vortex, and small centrifuge (if it is not already there, bring it to the NGS side of the lab bench - the blue one with the plastic lid).
SECTION: Setup
4. Get out a number of Qubit tubes (glass cabinet above the NGS workspace) equal to your number of samples + 2 for the standards (N tubes + 2). Label each with an alcohol resistant marker, and write down each sample in your lab notebook (if you are using an abbreviated label, note this as well).
SECTION: Master Mix
5. Each time you use the Qubit, you will need to prepare enough of the master mix for all the samples. Prepare 199 uL Qubit buffer + 1 uL of Qubit reagent * (N samples + 2 standards + 1 extra). For example, if you have 6 samples, you will need 199 uL Qubit buffer + 1 uL of Qubit reagent * (9) for all the tubes. Get out an appropriate number of tubes to fit the master mix and label them. Combine the amount of buffer and reagent necessary, then vortex and spin the master mix.  

SECTION: Scale Protocol
1. Double check that you have appropriately scaled this protocol. After clicking "run" above, you will need to enter your number of samples + 2 standards + 1 extra for pipette error (e.g. if you are running 6 samples, scale this protocol for 9).
SECTION: Making Tubes
6. For Standard tubes, pipette: 190 uL of Master Mix + 10 uL of standard into each tube. Do this for each separate standard, switching pipette tips for each one. Vortex and spin once done.
SECTION: Making Tubes
7. For DNA tubes: pipette 198 uL of Master Mix + 2 uL of extraction sample. Repeat for each sample, changing pipette tips in between each sample. Vortex and spin tubes once done.  
SECTION: Qubit
8. Start up the Qubit machine. 
Select the necessary path, which usually is: DNA > dsDNA broadrange. When told to run new standards, click yes
SECTION: Qubit
9. Enter standard 1 when prompted, then run. Enter standard 2 when prompted, then run. Repeat with DNA samples until done.
TITLE: Study_Protocol_English_Wintermann_V2
AUTHORS: gloria.wintermann

[DESCRIPTION]
Objectives: Patients with Panic Disorder (PD) show an abnormal stress-induced functioning of the Hypothalamic-Pituitary-adrenal (HPA)-axis. Different protocols for stress induction are of rather low relevance for the psychotherapeutic treatment. In practice, interoceptive exposure is often realized as Low Intensity Exercise (LIE), as compared to an incremental cycle exercise test to exhaustion. Currently, it is not known, whether LIE displays an effective interoceptive stressor 1.) leading to a significant anxiety induction; 2.) a comparable HPA- and Sympathetic-Adreno-Medullar (SAM)-axis response in both patients and healthy controls; 3.) stress responses under LIE are associated with treatment outcomes.
Patients and methods: N = 20 patients with PD and n = 20 healthy controls were exposed to ten minutes of LIE on an exercise bike. LIE was applied as part of the interoceptive exposure, during an intensive Cognitive-Behavioral Therapy (CBT) in a day clinic. Heart rate was monitored and salivary cortisol samples collected. Before and after the LIE, state anxiety/ arousal were assessed. In order to evaluate psychopathology, the Panic and Agoraphobia Scale, Mobility Inventory, Agoraphobic Cognitions Questionnaire and Body Sensations Questionnaire were applied, before (T1) and after five weeks (T2) of an intensive CBT.
Results: LIE led to a significant and similar heart rate increase in both groups. Cortisol decreased over time in both groups, especially in male patients. A higher psychopathology before, and after CBT, was associated with a significantly lower cortisol response under LIE.
Conclusions: In the present study, LIE led to a divergent stress response: while there was a significant heart rate increase, cortisol decreased over time, particularly in male patients. A lower reactivity of the HPA-axis seems to be associated with a lower treatment outcome, which may affect extinction based learning. The findings suggest, that interoceptive stimuli should be designed carefully in order to be potent stressors.

[STEPS]