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[DESCRIPTION]
<p>This protocol is for the extraction and purification of up to 10ug of plasmid DNA. It can be used for the purification of plasmid DNA ranging from 40 bp to 40kbp. </p>

[BEFORE_START]
<p>1) Add RNAse A solution to the bottle containing Solution 1 and mix well. Once RNAse A solution is added to solution 1, the resulting solution is stable for 6 months at 4<sup>o</sup>C. If being used infrequenly the solution can be stored at -20 <sup>o</sup>C for longer periods as long as freeze thaw cycles are minimized. </p>
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<p>2) If solution 2 contains a precipitate, dissolve the precipitate before use by gently warming the solution at 37 <sup>o</sup>C.</p>
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<p>3) Before using the wash solution, add 80 mL of 96-100% Ethanol to the 20 mL wash solution. Anhydrous Ethanol from Bio Basic Inc. (<a href="http://store.biobasic.com/product/d0193-size-500ml/" target="_blank">D0193</a>) is suitable for use with this protocol.</p>
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[GUIDELINES]
<p><strong>Introduction</strong></p>
<p><br />The EZ-10 Spin Column Kits provide a simple and efficient method for purification of plasmid DNA, extraction of DNA from agarose gels, and purification of DNA from enzymatic reactions such as PCR or restriction enzyme digestions.</p>
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<p>The DNA is selectively adsorbed in silica gel-based EZ-10 column and other components are washed away. The DNA is then eluted off the column and can be used for any downstream applications. </p>
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<p>The purification method used in these protocols does not require use of phenol, chloroform, or CsCl. The DNA is purified without an additional step of ethanol precipitation.</p>
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<p><strong>Limitations</strong> <strong>of Use</strong></p>
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<p>These kits are designed for research use only. The purified plasmid DNA should not be used for live animal transfections. It is also not to be used for human diagnostic or drug production purposes. </p>
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<p><strong>Features</strong></p>
<ul>
<li>Simple, Fast and Efficient</li>
<li>Preparation  of  high  quality  DNA  which  can  be used  in  any  downstream  applications  such  as sequencing,  PCR,  transformation  or  restriction digestions</li>
<li>HighYield and Reproducible</li>
<li>High Capacity -Up to 10μg of DNA per column</li>
</ul>
<p><strong>Storage</strong></p>
<p>EZ-10  Spin  Column  Kits  should  be  stored  dry  at room  temperature  (15 °C-25 °C).  Kits  can  be  storedfor  up  to  24 months without showing any reduction in performance and quality. RNase A stock solution can  be  stored  for  2  years  at  4 °C.  After  addition  of RNase A, Solution I is stable for 6 months at 4 °C. </p>
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<p><strong>Quality Control</strong></p>
<p>Each lot of EZ-10 Spin Column kit is tested against predetermined  specifications to ensure consistent product quality.  </p>
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<p><strong>Principle</strong>:</p>
<p>This kit provides a simple and efficient method formini plasmid DNA purification. The plasmid DNA is selectively adsorbed in silica gel-based EZ-10 column and other impurities such as proteins, salts,nucleotides, oligos (&lt;40-mer) are washed away. In order to maximize the recovery yield of plasmid DNA, a color indicator-VisualLyse is added to the buffer which prevents insufficient or over-lysis during lysis and neutralization step. The plasmid DNA is then eluted off the column and can be used for any downstream application.</p>

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