TITLE: Pesticide Analysis Autotrace SOP
AUTHORS: Lara Frankson

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">To test for neonics in turf runoff.</div></div>

[STEPS]
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
Turn on the system (doesn’t need to be turned off unless idle for >14 days)Open the nitrogen valve on the tank (to the right of the -40C freezer).Make sure the pressure on the regulator is no more than 100 psi.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
Using the regulator knob on the left side of the Autotrace (in the panel with a glass window), adjust the system’s output gas pressure to 10 psi.Pull knob out slightly and turn. This tends to come off so have patience with it.When adjusted, push back in and close the glass door.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
Top off solvents and check that lines are correctly in place.Take care that all solvent lines are amply submerged in the solvent and secure the lines in place with tape if necessary.Solvent line configuration is as follows:         i.     Line #1 = methanol         ii.     Line #2 = Milli-Q         iii.     Line #3 = methylene chloride (DCM)         iv.     Line #4 = 2% formic acid in waterIf you need more 2% formic acid in water, add approximately 400mL of Milli-Q water to a 500 mL volumetric flask. Pipette exactly 10 mL of concentrated formic acid into the flask. Bring to volume with Milli-Q water. Cap and invert to mix.Solvent line configuration is as follows:         i.     Line #1 = methanol         ii.     Line #2 = Milli-Q         iii.     Line #3 = methylene chloride (DCM)         iv.     Line #4 = 2% formic acid in waterIf you need more 2% formic acid in water, add approximately 400mL of Milli-Q water to a 500 mL volumetric flask. Pipette exactly 10 mL of concentrated formic acid into the flask. Bring to volume with Milli-Q water. Cap and invert to mix.         i.     Line #1 = methanol         ii.     Line #2 = Milli-Q         iii.     Line #3 = methylene chloride (DCM)         iv.     Line #4 = 2% formic acid in water
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
Check that the waste containers are not full or will not fill during extraction. There is also one waste container in the solvent rack. IF they are full:
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
a.     Solvent waste – prepare for hazardous waste pick up (CHLORINATED)
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
b.     Water waste – adjust pH to 6-8 with sodium bicarbonate and then dump down the sink, flushing with plenty of water.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
Rinse the Cleaning Cartridges with Milli-Q water in a squirt bottle
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
a.     This prevents leakage around the cartridge holder O-ring due to the presence of packing debris on the inside walls of the cartridge.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
Place the Cleaning Cartridges in each of the Autotrace holders by sliding the cartridge upward onto the bottom of the cartridge holder’s plunger.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
Push down on the lever until the assembly clicks into place and the holder’s LED comes on. (2nd one is dimly lit)
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
Purge air from the solvent lines by running the Prime Solvents method (method 29).
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
a.     Press LOAD to display the LOADING METHOD screen.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
b.     Press LOAD to increment the displayed method to 29.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
c.      Press CONT once to select method 29.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
d.     Press CONT to run method 29.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
e.     Watch the Liquid Handling Syringe on the left side of the instrument while this happens. On the 1st run, it likely won’t pull the full 7 mL of liquid per solvent.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
10.  Repeat step 9
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
a.     On the 2nd run, ensure that the Liquid Handling Syringe pulls at least 6 mL of liquid per solvent. If not, run the Prime Solvents method a 3rd time.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
11.  Place all of the sample lines in a container with MeOH.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
a.     Make sure there is enough solvent for the method and make sure that all of the sample lines are fully submerged.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
12.  Clean the sample lines by running the Clean Sample Lines method (method 2).
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
a.     Press LOAD to display the LOADING METHOD screen.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
b.     Press LOAD to increment the displayed method to 2.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
c.      Press CONT once to select method 2.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
d.     Press CONT to run method 2.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
e.     It will ask you to put all of the sample lines in a beaker with MeOH. You did this in step 10, so press CONT.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
f.       It will stop after running the MeOH. Then, place all of the sample lines in a beaker with Milli-Q Water. Press Cont.
?. [Prepare Autotrace for T120 Runoff Extractions (Neonics)]
Safety Equipment:Gloves, googles, lab coatLab Equipment:AutotraceNitrogen, industrial gradeMethanol, ACS gradeMilli-Q waterMethylene chloride (dichloromethane), HPLC grade2% formic acid in Milli-Q waterCleaning Cartridges (labeled Cleaning Cartridge)Lab Equipment:AutotraceNitrogen, industrial gradeMethanol, ACS gradeMilli-Q waterMethylene chloride (dichloromethane), HPLC grade2% formic acid in Milli-Q waterCleaning Cartridges (labeled Cleaning Cartridge)