TITLE: Sensor Protocol updated
AUTHORS: s.yao 

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The CoV-SARS-2 Antigen Diagnostic kit is a test intended for the qualitative detection of intact SARS-CoV-2 virions (SARS-CoV-2 is abbreviated as SARS-2 herein) in upper and lower respiratory specimens (such as nasopharyngeal or oropharyngeal swabs, sputum, lower respiratory tract aspirates, bronchoalveolar lavage, and nasopharyngeal wash/aspirate or nasal aspirate) collected from individuals for COVID-19 diagnosis.</div></div>

[STEPS]
?. [Results and data interpretation]
For testing the sensor and fluorometer using negative control and positive controlCalculate an average for the five values collected per run at 30sec and 10mRecord the difference in the average fluorescence reading at 10m from 30sec for negative sample this is the Vneg value.Record the difference in the average fluorescence reading at 10m from 30sec for positive sample, this is the Vpositive value.Calculate the % increase in fluorescence signal intensity from Vneg to Vpos. If the % change is greater than 15%, proceed to evaluation of patient samples. If the difference is For test samples:Calculate an average for the five values collected per run at 30sec and 10mRecord the difference in the average fluorescence reading at 10m from 30sec for Blank, this is the Vcontrol value.Record the difference in the average fluorescence reading at 10m from 30sec for test sample, this is the Vsample value.Calculate the background subtracted fluorescence count for the patient sample using the formula (((Vsample – Vcontrol)/ Vcontrol)*100). If the % change is greater than 15 % then the sample is positive for intact SARS-CoV-2 virions.For test samples:Calculate an average for the five values collected per run at 30sec and 10mRecord the difference in the average fluorescence reading at 10m from 30sec for Blank, this is the Vcontrol value.Record the difference in the average fluorescence reading at 10m from 30sec for test sample, this is the Vsample value.Calculate the background subtracted fluorescence count for the patient sample using the formula (((Vsample – Vcontrol)/ Vcontrol)*100). If the % change is greater than 15 % then the sample is positive for intact SARS-CoV-2 virions.
?. [Test the sample]
?. [Run positive and negative control]
Before each batch of sample testing,  it is recommended to run positive control and negative control to ensure the DNA Star sensor is functional.Incubate the PCR test for 20 min (every test contains 20uL of the DNA start assay solution)Set up the AND1100 fluorometer for test (refer to description on how to run test on fluorometer)Add 80uL of the blank/positive/negative solution to the PCR tube containing DNA star assay and start the timer  Quickly mix the solution gently by rotating the PCR tube in and up and down motion with your hand. DO NOT VORTEX OR MIX WITH PIPETTETo record the fluorescence, insert the PCR tube into the provided Fluorometer device adapter, make sure the tube is securely positioned so that the tube cannot move  Close the cap of the chamber and record the fluorescence reading after 30 seconds and 10m  of incubation timeSave your data in Excel (described in How to use Fluorometer section) and analyze data (described in data interpretation section) to ensure (Vpos-Vneg)/Vneg>15%. If the correct control results are not obtained, do not perform patient tests or report patient results. Contact Atom Bioworks Technical Support during normal business hours before testing patient specimens.Quickly mix the solution gently by rotating the PCR tube in and up and down motion with your hand. DO NOT VORTEX OR MIX WITH PIPETTETo record the fluorescence, insert the PCR tube into the provided Fluorometer device adapter, make sure the tube is securely positioned so that the tube cannot move  Close the cap of the chamber and record the fluorescence reading after 30 seconds and 10m  of incubation timeSave your data in Excel (described in How to use Fluorometer section) and analyze data (described in data interpretation section) to ensure (Vpos-Vneg)/Vneg>15%. If the correct control results are not obtained, do not perform patient tests or report patient results. Contact Atom Bioworks Technical Support during normal business hours before testing patient specimens.Close the cap of the chamber and record the fluorescence reading after 30 seconds and 10m  of incubation timeSave your data in Excel (described in How to use Fluorometer section) and analyze data (described in data interpretation section) to ensure (Vpos-Vneg)/Vneg>15%. If the correct control results are not obtained, do not perform patient tests or report patient results. Contact Atom Bioworks Technical Support during normal business hours before testing patient specimens.
?. [Preparation of tests]
Prepare the following three groups of testsBlank: Prepare a 100uL of blank solution by adding 10uL of the virus matrix (Matrix in which the samples to be tested are stored; can be PBS/Saliva/Water etc.) in 90uL of the bufferPositive and Negative controls: Prepare a 100uL of positive control/negative control solution by adding 10uL of the positive /negative control provided in the kit in 90uL of the buffer provided in the kitTest Sample: Prepare a 100uL of test sample by adding 10uL of patient sample in 90uL of the buffer provided in the kit