TITLE: EPCAM Sorting Protocol
AUTHORS: Morrisey Lab

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Epcam sorting Protocol</span></div><div class = "text-block"><div class = "justify" style = "text-align:justify"><span style = "font-weight:bold;">FACS Buffer: </span></div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">- 1L dPBS (Cell Center, cat# 14190136, without Ca++ & Mg++)</div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">- 2mL EDTA (Invitrogen, cat# 15575020, UltraPure, 0.5M, ph 8.0, 100mL)</div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">- 10mL of FBS (Invitrogen, cat# 10437-028, 500mL)</div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify"><span style = "font-weight:bold;">0.04% BSA+PBS:</span></div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">- 10mg BSA (Jackson, cat# 001-000-162, IgG free, protease free)</div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">- 25mL PBS (Cell Center, cat# 14190136, without Ca++ & Mg++)</div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify"></div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify"><span style = "font-weight:bold;">MACS Accessories: (Miltenyi Biotec):</span></div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">QuadroMACS Separator (cat# 130-090-976)</div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">MACS multistand (cat# 130-042-303)</div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">MACS LS Cloumns (25pk) (cat# 130-042-401)</div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">MACS EpCAM microbeads, human (cat#130-061-101)</div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">Cellometer K2 (Nexcelome)</div><style>
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							    width: 100%;
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">Countess™ Cell Counting Chamber Slides (Thermo Fisher cat#C10228)</div><style>
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							    width: 100%;
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">ViaStain™ AOPI Staining Solution (Nexcelom/ Fisher cat #CS2-0106)</div><style>
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							    width: 100%;
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">Evos (Lifetech)</div><style>
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							</style></div><div class = "text-block"><div class = "justify" style = "text-align:justify">Trypan Blue (Fisher cat #ICN1691049)</div><style>
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[STEPS]