TITLE: 1DROP SARS-CoV-2 antigen test
AUTHORS: Mahe Raccaud, Manon Garzuel, Joerg Ziegler, Luc Gervais

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Our capillary-driven microfluidic chips automatically detect multiple SARS-CoV-2 antigens after the addition of a few drops of saliva, triggering a cascade of events powered by capillary forces. We integrate biochemical reagents directly on the microfluidic chips, avoiding the need for peripheral equipment for fluid control. Thus, detection of SARS-CoV-2 can be done at the patient’s side by simply adding sample to the chips, with minimal hands-on time, faster results, and increased sensitivity of fluorescence assays. The use of saliva as a sample matrix avoids painful sample-collection procedures and the need for trained medical staff.</div></div>

[STEPS]
?. [Sample collection]
Collect patient saliva in the Collection Tube. Saliva must be collected until the amount reaches above the  line.
1 mL
?. [Device preparation]
Switch on the 1DROP Analyzer with the side switch.
?. [Device preparation]
Scan the Chip on the scanner of the 1DROP Analyzer.
?. [Device preparation]
Follow instructions on the screen to enter patient ID.
?. [Sample preparation]
Use the Pipette to transfer  of saliva to the Reagent Tube containing the Buffer. To transfer the patient sample: Firmly squeeze the top bulb of the Pipette. Still squeezing, place the Pipette tip into the saliva sample. With the Pipette tip still in the sample, release pressure on the bulb to fill the Pipette up to   Place the Pipette in the Reaction Tube containing the Buffer. Firmly squeeze the bulb to empty the contents of the Pipette in the Reaction Tube.With the Pipette tip still in the sample, release pressure on the bulb to fill the Pipette up to Place the Pipette in the Reaction Tube containing the Buffer. Firmly squeeze the bulb to empty the contents of the Pipette in the Reaction Tube.
500 µl
500 µl
?. [Sample preparation]
Pipette up and down the reagent mix in the Reagent Tube 5 times.
?. [Test Procedure]
Just prior to loading the sample, pipet up and down two more times the prepared sample in the Reagent Tube.
?. [Test Procedure]
Fill the Pipette and transfer 4 drops to the loading pad of the Chip.
?. [Test Procedure]
Once the sample have been loaded, start a timer for .
?. [Test Procedure]
After 15 minutes, insert the Chip in the 1DROP Analyzer slit and press "Start" on the screen. The result will be displayed on the 1DROP Analyzer screen.    AB1DisplayInterpretation2CoV2 PositivePositive Test for SARS-CoV-2 (antigen detected).3CoV2 NegativePresumptive Negative Test for SARS-CoV-2 (no antigen detected).4Invalid TestTest is invalid.** Repeat the test with another Chip (same sample mix can be reused).
AB1DisplayInterpretation2CoV2 PositivePositive Test for SARS-CoV-2 (antigen detected).3CoV2 NegativePresumptive Negative Test for SARS-CoV-2 (no antigen detected).4Invalid TestTest is invalid.*
?. [Test Procedure]
Once the results have been read, remove the Chip from the 1DROP Analyzer and switch off the Analyzer.
?. [Test Procedure]
Once done, discard the Chip, the Pipette, the Collection Tube, and the Reagent Tube as biohazard waste.