TITLE: SN medium
AUTHORS: Roscoff Culture Collection

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Used for some cyanobacteria in particular </span><span style = "font-style:italic;">Synechococcus</span><span style = "font-style:italic;">.</span></div></div>

[STEPS]
?. Autoclave and filter aged seawaterWork under laminar flow hood.Add to 800mL of seawater the following nutriments that have been autoclaved beforehand  (except vitamin)     Quantity
   Compound
   Stock Solution
     2,0 mL
   EDTA disodium salt dihydrate (Na2EDTA-2H2O)
   2,8 g/L
     2,0 mL
   Sodium carbonate dihydrate (Na2CO3-H2O)
   6 g/L
     2,0 mL
   Sodium nitrate (NaNO3)
   106,2 g/L
     2,0 mL
   Dipotassium phosphate (K2HPO4)
   3,05 g/L
     250 µL
   Ammonium chloride (NH4Cl)
   21,4 g/L
      
 250 µL
   Cyano Trace Metals (see receipe below)
    
     250 µL
   Cyanocobalamin (Vit. B12)
   8 µg/L
     200mL
   Steril milliQ water
    
     2,0 mL
   Sodium sulfite (Na2SO3)
   126 g/L
     Filter the medium on 0.2µm (do not autoclave)  ABC1Quantity
Compound
Stock Solution
22,0 mL
EDTA disodium salt dihydrate (Na2EDTA-2H2O)
2,8 g/L
32,0 mL
Sodium carbonate dihydrate (Na2CO3-H2O)
6 g/L
42,0 mL
Sodium nitrate (NaNO3)
106,2 g/L
52,0 mL
Dipotassium phosphate (K2HPO4)
3,05 g/L
6250 µL
Ammonium chloride (NH4Cl)
21,4 g/L
7&nbsp;
250 µL
Cyano Trace Metals (see receipe below)
&nbsp;
8250 µL
Cyanocobalamin (Vit. B12)
8 µg/L
9200mL
Steril milliQ water
&nbsp;
102,0 mL
Sodium sulfite (Na2SO3)
126 g/L
ABC1Quantity
Compound
Stock Solution
22,0 mL
EDTA disodium salt dihydrate (Na2EDTA-2H2O)
2,8 g/L
32,0 mL
Sodium carbonate dihydrate (Na2CO3-H2O)
6 g/L
42,0 mL
Sodium nitrate (NaNO3)
106,2 g/L
52,0 mL
Dipotassium phosphate (K2HPO4)
3,05 g/L
6250 µL
Ammonium chloride (NH4Cl)
21,4 g/L
7&nbsp;
250 µL
Cyano Trace Metals (see receipe below)
&nbsp;
8250 µL
Cyanocobalamin (Vit. B12)
8 µg/L
9200mL
Steril milliQ water
&nbsp;
102,0 mL
Sodium sulfite (Na2SO3)
126 g/L
?. Cyano Trace Metal SolutionDissolve all these components separately in milliQ water     Quantity
   Compound
     6,25 g
   Citric Acid·H2O
     6,0 g
   Ferric Ammonium Citrate
     1,4 g
   Manganese (II) chloride tetrahydrate (MnCl2-4H2O)
     0,39 g
   Sodium molybdate dihydrate (Na2MoO4-2H2O)
     0,025 g
   Cobalt nitrate hexahydrate (Co(NO3)2-6H2O)
     0,222 g
   Zinc sulfate heptahydrate (ZnSO4-7H2O)
     Combine the various solutions after full dissolutionMake final volume up to 1L with milliQ waterStore in refrigerator  AB1Quantity
Compound
26,25 g
Citric Acid·H2O
36,0 g
Ferric Ammonium Citrate
41,4 g
Manganese (II) chloride tetrahydrate (MnCl2-4H2O)
50,39 g
Sodium molybdate dihydrate (Na2MoO4-2H2O)
60,025 g
Cobalt nitrate hexahydrate (Co(NO3)2-6H2O)
70,222 g
Zinc sulfate heptahydrate (ZnSO4-7H2O)
Combine the various solutions after full dissolutionMake final volume up to 1L with milliQ waterStore in refrigerator
AB1Quantity
Compound
26,25 g
Citric Acid·H2O
36,0 g
Ferric Ammonium Citrate
41,4 g
Manganese (II) chloride tetrahydrate (MnCl2-4H2O)
50,39 g
Sodium molybdate dihydrate (Na2MoO4-2H2O)
60,025 g
Cobalt nitrate hexahydrate (Co(NO3)2-6H2O)
70,222 g
Zinc sulfate heptahydrate (ZnSO4-7H2O)