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[DESCRIPTION]
This is a protocol for high yield recovery of pure DNA from agarose gels using the Zymoclean&trade; Gel DNA Recovery Kit.

[BEFORE_START]
Add 24 ml 100% ethanol (26 ml 95% ethanol) to the 6 ml <strong>DNA Wash Buffer</strong> concentrate. Add 96 ml 100% ethanol (104 ml 95% ethanol) to the 24 ml <strong>DNA Wash Buffer</strong> concentrate.

[GUIDELINES]
All centrifugation steps should be performed between 10,000 - 16,000 x g.<br /><br /><strong>Product Contents</strong><br />
<table>
<tbody>
<tr>
<td><strong>Zymoclean&trade; Gel DNA Recovery Kit</strong> (Kit Size)</td>
<td><strong>D4001, D4007 </strong>(50 Preps.)</td>
<td><strong>D4002, D4008 </strong>(200 Preps.)</td>
<td><strong>Storage Temperature</strong></td>
</tr>
<tr>
<td><strong>ADB</strong></td>
<td>50 ml</td>
<td>2x100 ml</td>
<td>Room Temp.</td>
</tr>
<tr>
<td><strong>DNA Wash Buffer<sup>1</sup></strong></td>
<td>6 ml</td>
<td>24 ml</td>
<td>Room Temp.</td>
</tr>
<tr>
<td><strong>DNA Elution Buffer</strong></td>
<td>1 ml</td>
<td>4 ml</td>
<td>Room Temp.</td>
</tr>
<tr>
<td><strong>Zymo-Spin&trade; I Columns</strong></td>
<td>50 <br />D4001 &ndash; uncapped columns <br />D4007 &ndash; capped columns</td>
<td>200<br />D4002 &ndash; uncapped columns <br />D4008 &ndash; capped columns</td>
<td>Room Temp.</td>
</tr>
<tr>
<td><strong>Collection Tubes</strong></td>
<td>50</td>
<td>200</td>
<td>Room Temp.</td>
</tr>
<tr>
<td><strong>Instruction Manual</strong></td>
<td>1</td>
<td>1</td>
<td>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; -</td>
</tr>
</tbody>
</table>
<em>Integrity of kit components is guaranteed for up to one year from date of purchase. Reagents are routinely tested on a lot-to-lot basis to ensure they provide the highest performance and reliability.</em> <br /><sup>1</sup> Ethanol must be added prior to use as indicated on the DNA Wash Buffer label. <br /><br /><strong>Specifications</strong> <br />&bull; <strong>DNA Purity</strong> &ndash; High-quality, purified DNA is especially well suited for sequencing and ligation reactions. <br />&bull; <strong>DNA Size Limits</strong> &ndash; From ~50 bp to 23 kb. <br />&bull; <strong>DNA Recovery</strong> &ndash; Typically, up to 5 &micro;g total DNA per column can be eluted into as little as 6 &micro;l of low salt <strong>DNA Elution Buffer</strong> or water. For DNA 50 bp to 10 kb, the recovery is 70-90%. For DNA 11 kb to 23 kb, the recovery is 50-70%. <br />&bull; <strong>Sample Sources</strong> &ndash; DNA in excised agarose gel slices. <br />&bull; <strong>Product Detergent Tolerance</strong> &ndash; &le; 5% Triton X-100, &le; 5% Tween-20, &le; 5% Sarkosyl, &le; 0.1% SDS.&nbsp;<br /><br /><strong>Product Description</strong><br /> The<strong> Zymoclean&trade;</strong> <strong>Gel DNA Recovery Kit</strong> provides a hassle-free method for high yield recovery of pure DNA from agarose gels. Simply add the specially formulated <strong>Agarose Dissolving Buffer (ADB)</strong> to the gel slice containing your DNA sample, let dissolve, and then transfer to the supplied <strong>Zymo-Spin&trade; Column</strong>. There is no need for organic denaturants or chloroform. Instead, the product utilizes Fast-Spin column technology to yield high-quality DNA in just 15 minutes (See figures below). DNA purified using the <strong>Zymoclean&trade; Gel DNA Recovery Kit</strong> is perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.<br /><br /><img src="https://s3.amazonaws.com/pr-journal/ijc3kn.png" alt="" width="536" height="239" /><br /><strong>Zymoclean&trade;</strong> products are offered in single column (uncapped or capped column) or 96-well format. In addition, the <strong>Zymoclean&trade; Large Fragment DNA Recovery Kit</strong> is designed for large DNA (up to 200 kb) gel recovery.<br /><br /><img src="https://s3.amazonaws.com/pr-journal/ipc3kn.png" alt="" width="520" height="237" /><br /><br />

[STEPS]
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