eamag/protocols-with-steps huggingface dataset:
TITLE: Myco Nootropic Brain Gummies:-Does it really work? Review after 30 days Use
AUTHORS: brainmyconootropic  

[DESCRIPTION]
Myco Nootropic Brain Gummies is a versatile high-potency nootropic supplement manufactured in the UK and USA by Wolfson Brands. In common with all the other top nootropic products, Myco Nootropic Brain Gummies is an oral supplement that comes in the form of a gummies.

[STEPS]
TITLE: FASTQ alignment, gene counts, and differential expression analysis and functional annotation of DEGs
AUTHORS: Rebecca Wallings

[DESCRIPTION]
FASTQ alignment, gene counts, and differential expression analysis and functional annotation of DEGs

[STEPS]
SECTION: FASTQ alignment, gene counts, and differential expression analysis
1. FASTQ files were aligned against the mouse genome (GRCm39) and GRCm39.107 annotation using STAR to generate BAM files. Gene counts were generated from BAM files using Rsamtools (Bioconductor package) and the summarizeOverlaps function with the GenomicAlignments package v1.36.0. Differential gene expression analysis was performed with DESeq2 package v1.40.2 using the “DESeq” function with default settings which fits a generalized linear model for each gene. Subsequent Wald test P-values are adjusted for multiple comparisons using the Benjamini–Hochberg method (adjusted P-value). Pair-wise changes in gene expression levels between groups were used to identify DEGs. DEGs will be defined as an absolute log2 fold change ≥0.5 and an adjusted P-value ≤0.05. 
SECTION: Functional annotation of DEGs
2. Gene Ontology (GO) enrichment analysis was performed with goseq to identify enrichment in gene ontology categories and KEGG pathways. For DEGs, up- and down-regulated gene lists were analyzed separately. Over-represented P-values were adjusted for multiple comparisons using the
Benjamini–Hochberg (BH) adjustments for controlling false-discovery rates. An enrichment score was calculated using an observed-over-expected ratio for each gene list. GO-BP categories and KEGG pathways were plotted if their BH adjusted FDR reached >= 0.05 and the number of DEGs within each category/pathway was greater than 4. To eliminate larger, broad GO-BP parent categories, categories with more than 250 total genes were not plotted. To eliminate redundancy, pathways with significant overlap regarding genes and semantics were merged.
TITLE: Large (Klenow) Fragment Blunting (M0210)
AUTHORS: New  England   Biolabs

[DESCRIPTION]
Protocol for blunting ends by 3' overhang removal and fill-in of 3' recessed (5' overhang) ends using DNA Polymerase I, Large (Klenow) Fragment (M0210)

[STEPS]
1. DNA should be dissolved in 1 X or T4 DNA Ligase Reaction buffer supplemented with 33 Micromolar (µM) each dNTP.
2. Add 1 unit of Klenow per microgram DNA.  
3. Incubate for 15 min at 25 °C. 
4. Stop reaction by adding EDTA to a final concentration of 10 Milimolar (mM).
5. Heat for 20 min at 75 °C.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<p>The protocol aims explicitly to amplify BFV viruses and not other viruses.</p>
<p>The assay targets the E2 gene region and is designed as a qualitative test for investigating BFV infection of humans and arthropods.</p>
<p> </p>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>

[BEFORE_START]
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<ul>
<li>If using a different brand or model of real-time thermocycler, check the concentration of ROX is adequate.</li>
<li>Method assumes the user is familiar with the thermocycler and software used to run the protocol and with PCR in general.</li>
</ul>
</div>
</div>
</div>
</div>
</div>
</div>
</div>

[GUIDELINES]
<div> </div>

[STEPS]
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TITLE: Padrões de arquivamento para Revisões Sistemáticas e Quantitativas da Literatura (RSQL)
AUTHORS: Daniel  Vartanian

[DESCRIPTION]
Este protocolo foi criado para o programa de Revisões Sistemáticas e Quantitativas da Literatura (RSQL) científica do Grupo Interdisciplinar de Pesquisa em Sono (GIPSO), mas ele pode ser utilizado por qualquer um que queira realizar uma RSQL. O programa foi criado para que novos ingressantes do GIPSO possam dar um pontapé inicial em uma carreira acadêmica na cronobiologia. Saiba mais no protocolo abaixo.
 
Para dúvidas ou sugestões, entre em contato em gipso@usp.br.

[STEPS]
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This year´s interlab study allows participants to fulfil Bronze medal requirements. </p>
<p>For this purposes a set of experiments has to be performed, which in the end will be compared and validated with other team´s data. </p>
<p>Part of this Challenge are the Calibration protcols. </p>
<p>For the third calibration, a fluorescein standard curve is prepared and fluorescence (bandpass width: 520nm/30nm bandpass, 25-30nm width; excitation; 485nm; emission: 520-530nm) is measured. </p>

[BEFORE_START]
<h3> </h3>
<div>
<div>
<div>
<div>
<div>
<div>
<p>Make sure to adjust the temperature of your plate reader to room temperature (22°C-25°C) before measurement.</p>
</div>
</div>
</div>
</div>
</div>
<div> </div>
<div>Also prepare the fluorescein stock solution before starting. Follow the steps in this protocol. </div>
</div>

[GUIDELINES]
<p>Make sure to always use the same plates, volumes and settings for the measurement as will be used for the other calibrations and the cell measurements. </p>
<p>Also take care to remain constant with your pipetting techniques. </p>

[STEPS]
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TITLE: Post-IMS H&E Staining for Pancreas or Eye Cryosections
AUTHORS: Angela   Kruse, Diane  Saunders, Jamie  Allen, Carrie   Romer, Danielle  Gutierrez, Alvin Powers, Jeff  Spraggins

[DESCRIPTION]
This protocol for H&E staining can be applied to either fixed or unfixed frozen cryosections.

[STEPS]
SECTION: H&amp;E Staining
3. Incubate in 95% alcohol for 1 min , then wash, dipping until clear.

SECTION: H&amp;E Staining
4. Incubate in hematoxylin for 30 s , then wash until clear.

SECTION: H&amp;E Staining
5. Dip 3-4x in bluing solution, then wash for 30 s 

SECTION: H&amp;E Staining
6. Dip 3-4x in 95% alcohol.
SECTION: H&amp;E Staining
7. Dip 1-2x in eosin.
SECTION: H&amp;E Staining
8. Dip 5-10x in 95% alcohol, then repeat using 2nd aliquot.
SECTION: H&amp;E Staining
9. Dip 5-10x in 100% alcohol, then repeat using 2nd aliquot.
SECTION: H&amp;E Staining
10. Dip 5-10x in xylene, then repeat using 2nd aliquot.
SECTION: H&amp;E Staining
11. Apply coverslip and seal.

    

SECTION: Matrix Removal
1. Collect post-IMS autofluorescence
SECTION: Matrix Removal
2. Remove matrix from tissue sections by incubating in 100% alcohol, dipping gently for 30 s. Repeat this step 2-3 times until there is no visible matrix on the slides.
TITLE: Induction of starvation stress
AUTHORS: Louise Uoselis

[DESCRIPTION]
Induction of starvation stress using EBSS in HeLa cells

[STEPS]
SECTION: Day 1
1. Seed cells, aiming for a confluency of 90 – 100% at the time of treatment the next day. 

SECTION: Day 2
2. Feed cells for 60 min in an appropriate volume of standard growth media. 

SECTION: Day 2
3. Remove the media, and wash each well 3 times with an ample amount of room temperature PBS (eg. Wash with 3 mL of PBS per well of a 6 well plate). 
SECTION: Day 2
4. After the third wash, add an appropriate volume of Earle’s Balanced Salt Solution (EBSS) to each well/plate. 
SECTION: Day 2
5. After 480 min of incubation in EBSS, harvest all samples for analysis.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<a href="http://store.p212121.com/ampicillin-sodium-salt/" target="_blank">Ampicillin</a> is a &beta;-lactam antibiotic routinely used in bacterial selection procedures to select for bacteria&nbsp;(usually E. coli) that have been transformed with an ampicillin resistance plasmid (pUC19, others).&nbsp;Ampicillin resistance is&nbsp;usually due to production of beta-lactamase&nbsp;enzymes which cleave the beta -&nbsp;lactam ring rendering the antibiotic inactive.<br /><br />The information below will outline preparation, storage, and a general selection procedure for ampicillin resistant bacteria.

[STEPS]
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TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This protocol aims to achieve an isotopic labeling of mixotrophic algae using <sup>15</sup>N and <sup>13</sup>C labeled heat-killed bacteria. It was developped to be used with the mixorophic chrysophyte <em>Ochromonas</em> to asses the sources of carbon and nitrogen of this algae when growing mixotrophically . Sources of nitrogen and carbon in the media used were reduced to 1 inorganic source (NH<sub>4</sub>Cl for nitrogen and bicarbonate for carbon) and 1 organic source (heat-killed bacteria); media used for <em>Ochromonas</em> growth was modified accordingly to reflect this.</p>
<p> </p>
<p>Downstream analysis of isotopic labeled Ochromonas include bulk IRMS measurements or nanoSIMS measurements.</p>

[STEPS]
?. 
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TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>The presence and structure of EFNs in <em>Opuntia robusta</em> had not been investigated. We used light, scanning-electron, and transmission-electron microscopy to examine morphology, anatomy, and ultrastructure of the secretory spines in areoles in female and hermaphrodite individuals of <em>O. robusta</em>. Young cladodes develop areoles with modified and secretory spines as EFNs only active during the early growth phase of female and hermaphrodite individuals. EFNs are non-vascularized structures, with no stomata, that consist of three distinct tissues: a basal meristematic tissue; a middle elongation region; and an apical secretory cone (asc) formed by large globular epidermal cells, of sac shape, containing nectar and medullar elongated cells. TEM observations indicated the presence of Golgi apparatus, vesicles and plastids in the medullar and sup-epidermal cells of the asc, transporting nectar to the epidermal secretory cells. The nectar is transported through plasmodesmata, and then stored on the asc cells, to be secreted by breaking through the globular cells and pores</p>

[STEPS]
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TITLE: Synthesis of colloidal dextran-conjugated superparamagnetic iron nanoparticles (SPIONs)
AUTHORS: William  Hancock-Cerutti, Arun  Kumar Tharkeshwar, Shawn  M Ferguson, Pietro  De Camilli

[DESCRIPTION]
This method describes the synthesis and usage of dextran-conjugated superparamagnetic iron nanoparticles (SPIONs)


[STEPS]
TITLE: Sub-Cloning Primer Design and PCR
AUTHORS: Abby Jurgensmeier, Moriah Beck

[STEPS]
?. Combine the following in a small PCR tube:  AB11 uLForward Primer (10 uM)21 uLReverse Primer (10 uM)31uLTemplate DNA (may need adjustment for at least 20 ng; remember to adjust water volume!)41 uLdNTPs50.25 uLTaq Polymerase65 uL10X Thermopol Buffer741.75 uLddH20
AB11 uLForward Primer (10 uM)21 uLReverse Primer (10 uM)31uLTemplate DNA (may need adjustment for at least 20 ng; remember to adjust water volume!)41 uLdNTPs50.25 uLTaq Polymerase65 uL10X Thermopol Buffer741.75 uLddH20
?. PCR Conditions as programmed into thermocycler as BECKTAQT Denaturation    x30        Denaturation         Annealing         Elongation Final extension
[Adjust to 5 less than primer Tm]
TITLE: Rice medium
AUTHORS: Roscoff Culture Collection

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Used for heterotrophic protists.</div></div>

[STEPS]
?. Filter 1L of seawater aged for at least two months on prefilter and filter 0.2 µm
?. Heat seawater during 20 min at 100°C
?. Add 40 graism of rice
?. Autoclave 3 times the medium (let temperature decrease between each round of autoclaving)
TITLE: Optical densitometry of neuronal fibers
AUTHORS: Núria Peñuelas

[DESCRIPTION]
Optical densitometry of striatal neuronal fibers in the rodent brain

[STEPS]
1. Scan the immunostained serial coronal sections covering different caudorostral levels of the brain regions (for striatum, 4 sections/animal) with an Epson Perfection v750 Pro scanner.
2. Open the resulting images in ImageJ (RRID:SCR_003070,https://imagej.net/). 
3. With the free hand selections tool, draw the region of interest (e.g. striatum, nucleus accumbens and olfactory tubercle) and measure the optical density (pixel brightness). Then draw a region of interest in a blank area (e.g. cortex) and also measure the optical density.

4. Calculate the optical density or absorbance using the formula: -log10 (Striatum Intensity/Cortex Intensity).
TITLE: Feeding T75, T150, and 6wp Backup
AUTHORS: Andrea Argouarch

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol includes feeding of T75 flask, T150 flask, or the back up 6 well plate.</div></div>

[STEPS]
?. [Observations]
Observe cell morphology and outgrowth
?. [Observations]
Feed every 2-3 days until 90-100% confluent
?. [Preparation]
Turn off UV lights and clean hood with 70% ethanol
?. [Preparation]
Clean items with 70% ethanol and bring into hood    a. Sterile Filtered Media with PenStrep
?. [Preparation]
Write down observations on confluency
?. [Culturing]
Aspirate old media
?. [Culturing]
Add 20 mls   of media with penstrep to T75 flask
20 mL
?. [Culturing]
Add 2 mls   of media with penstrep to 6wp backup
2 mL
?. [Culturing]
Continue to feed every 2-3 days until 90-100% confluent
?. [Clean Up]
Throw away biohazard materials properly
?. [Clean Up]
Clean and sterilize hood with 70% ethanol and turn on UV
?. [Clean Up]
Update cell culture notes in lab notebook
?. [Culturing]
Add 30 mls   of media with penstrep to T150 flask
30 mL
TITLE: Enteric neuron activity during spontaneous motor complexes in mouse colon
AUTHORS: Kristen Smith-Edwards

[DESCRIPTION]
This protocol describes the steps to prepare mouse colon and image GCaMP calcium activity in myenteric neurons during spontaneous colonic motor complexes. 

[STEPS]
1. Euthanize EIIa-GCaMP mice (offspring from the pairing of B6.FVB-Tg(EIIa-cre)C5379Lmgd/J [RRID:IMSR_JAX:003724;cat. 003724; Jax Labs] and B6J.Cg-Gt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze/MwarJ [RRID:IMSR_JAX:028866;cat. 028865; Jax Labs]), by inhalation of isoflurane and thoracotomy. Remove the entire colon from mouse and place into a Sylgard-lined Petri dish (Dow Corning). Circulate oxygenated artificial cerebral spinal fluid at room-temp (ACSF, containing in mM: 117.9 NaCl [cat. S9888, Sigma], 4.7 KCL [cat. P3911; Sigma], 25 NaHCO3[catS6014; Sigma], 1.3 NaH2PO4, [cat. S8282; Sigma] 1.2 MgSO47H2O [cat. 230391; Sigma], 2.5 CaCl2 [cat. C1016; Sigma], 11.1 D-glucose [cat. G5767; Sigma], 2 sodium butyrate [cat. B5887; Sigma], and 20 sodium acetate [cat. S2889; Sigma]). Cut the colon open longitudinally and pin flat using minutien pins (Fine Science Tools; cat. 26002-20) with mucosal side facing down. A small amount of stretch should be applied when pinning the colon for optimal imaging conditions. The full-length, full-thickness colon should remain intact for imaging activity during spontaneous motor complexes. Transfer to the stage of an upright fluorescence microscope (DM6 FS, Leica) equipped with camera (Prime 95B, Photometrics, Roper Scientific) and software (Metamorph, Molecular Devices) for calcium imaging and slowly heat up circulating fluid to 35-37 C using a heated water bath.
2. After the colon has warmed up and equilibrated (10-15 minutes), use a 10X objective, focus on the myenteric neurons and image calcium signals during spontaneous motility behavior. Myenteric neurons can be distinguished from other cell types based on 1) their location in ganglia within the myenteric plexus between the longitudinal and circular muscle layers, 2) cell body shape and size, and 3) the kinetics of their calcium transients. Set imaging parameters to 150ms exposure time, 1200 frames, 2x2 binning to obtain 3-min duration imaging files. Collect several consecutive 3-min imaging files for a total of 9-15 min to capture multiple colon motor complexes (CMCs), which typically occur every 2-5 min depending on the amount of stretch applied to the colon tissue. 
3. Export imaging files to ImageJ and open as a multi-TIFF stack. Use the ImageJ plugin "Template Matching, Align Slices in Stack" to quantify and correct for tissue displacement in the x and y axis of the field of view. Save the x and y displacement values for all 1200 frames, convert frames to time by multiplying the frame by 0.150, and plot as a motility trace (displacement over time) in Excel. 
4. After imaging files have been corrected for movement in ImageJ and displacement data saved, myenteric neuron activity can be analyzed by drawing regions of interests over cell bodies and plotting the mean intensity changes over time.
TITLE: MED-JET H4 MULTIJET (MJH4M) Transfection Protocol
AUTHORS: Nathan Liu, Hani Abd-Ul-Salam, Noemie Joannette-Lafrance, Jingjing Li, Karim Menassa, Monzur Murshed

[DESCRIPTION]
Transfection, a non-viral method of nucleic acid delivery, often shows poor efficiency in vivo. The needle-based in vivo delivery of transfection reagents can be invasive. Here, we report a non-invasive in vivo gene delivery protocol via the needle-free MED-JET H4 MULTIJET (MJH4M) devices using glucose-based and commercial transfection reagents. The objective of this study is to compare the relative transfection efficiencies of the needle-free system to that of the needle-based delivery method. The highest transfection efficiency was noted using 5% glucose as a delivery agent; a 15-fold increase was observed using MJH4M compared to delivery with needles. 

[STEPS]
1. Prepare transfection reagent by diluting renilla-luciferase plasmid DNA (pR-Luc) in 5% glucose (w/v) to a final concentration of 0.1 μg/μl.
2. Insert the transfection reagent cartridge into the MED-JET H4 MULTIJET (MJH4M) device.
3. MJH4M device parameters were set to 80 psi and 75 µl.
4. Adult wild-type C57BL/6 mice were anesthetized via a mixture of inhaled isoflurane and oxygen gas.
5. The dorsal and ventral regions of the mice hind legs were shaved to expose the skin covering the region below the knee.
6. The injection landmark was identified on the mice hindleg ventral skin 7mm proximal to the heel.
7. The tip of the device is positioned directly on the injection landmark and the trigger was pressed. The device was held on the hindleg for 3 seconds to ensure retention of the injected liquid.
8. Tissue collection occurred 48 hours after the injection. The mice were euthanized using inhaled isoflurane and carbon dioxide followed by cervical dislocation.
9. Mice hindleg muscles were extracted from the bone and flash frozen in liquid nitrogen.
10. Luciferase assay and signal normalization were performed to determine the transfection efficiency.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Objective: This study aimed to determine the predictive value of the neutrophil to lymphocyte ratio (NLR) in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD).</p>
<p>    Methods: A retrospective study was conducted from March 2012 to May 2016 in Fuxing Hospital, Capital University of Medical Science. We collected 906 cases (525 males, 381 females, mean age 81.86±9.75 years) diagnosed with AECOPD. The NLR was calculated from their white blood cell (WBC), neutrophil (NEU), and lymphocyte (LYM)counts, which were obtained at laboratory examination.</p>
<p>    Result: After treatment, 698 patients with AECOPD improved. The NLR was higher at admission (6.89±6.82) than after treatment (4.19±5.11) (P=0.000). The area under the receiver operating characteristic curve (AUC) of the NLR for predicting the 28-day mortality rate was 0.737. Using 8.130 as the critical NLR value, the sensitivity was 60.5%, and the specificity was 74.8%. The AUC of the NLR for predicting the frequency of the need for invasive mechanical ventilation was 0.732. Using 10.345 as the critical NLR value, the sensitivity was 54.3%, and the specificity was 84.8%. The AUC of WBC, NEU and LYMfor predicting 28-day mortality and the need for invasive mechanical ventilation in these patients were all less than 0.7. An increased NLR was an independent risk factor for 28-day mortality (OR=1.067, P=0.000), intensive care unit occupancy (OR=1.046, P=0.000), and the need for invasive mechanical ventilation (OR=1.042, P=0.000). Compared with those patients without comorbidities, patients with renal dysfunction or upper gastrointestinal bleeding had an increased risk of death within 28 days (OR=3.102, OR=4.598, respectively), ICU admission (OR=2.228, OR=3.103, respectively), and the need for invasive mechanical ventilation (OR=3.572, OR=4.279, respectively).</p>
<p>    Conclusion: In patients with AECOPD, the accuracy of the NLR for predicting the 28-day mortality rate and frequency of the need for mechanical ventilation was significantly higher than the accuracy of WBC, NEU and LYMcounts. AECOPD patients with an NLR≥8.130 had higher 28-day mortality rate, while those with an NLR ≥10.345 were more likely to need invasive mechanical ventilation.</p>

[STEPS]
TITLE: K1 Keto Reviews -  (Ingredients, Side Effects) Does Pills Work Without Keto Diet!
AUTHORS: thomsalonzo  

[DESCRIPTION]
Why am I exhausted on K1 keto Life Diet?
K1 keto Life Reviews - The most widely recognized oftentimes asked question on the web. You probably paid attention to great weight reduction examples of overcoming adversity, how somebody loses in excess of 20 pounds weight with K1 keto LifeDiet, nobody let you know obstacles of K1 keto LifeDiet. On the off chance that you are a novice in the weight reduction cycle and need to accomplish the objective, you ought to peruse or take all precautionary measures to keep away from Keto Flu side effects or save you from the weariness of Ketogenic recipe.

[STEPS]
TITLE: Scanning electron microscopy
AUTHORS: Sue Lin

[DESCRIPTION]
<div class = "text-blocks"></div>

[STEPS]
?. Double fixation: The specimen was first fixed with 2.5% glutaraldehyde in phosphate buffer (pH7.0) for more than 4 hours; washed three times in the phosphate buffer; then postfixed with 1% OsO4 in phosphate buffer (pH7.0) for 1 hour and washed three times in the phosphate buffer.
?. Dehydration: The specimen was first dehydrated by a graded series of ethanol (50%, 70%, 80%, 90%, 95% and 100%) for about 15 to 20 minutes at each step, transferred to the mixture of alcohol and iso-amyl acetate (v:v=1:1) for about 30 minutes, then transferred to pure iso-amyl acetate for about 1 hour. In the end, the specimen was dehydrated in Hitachi Model HCP-2 critical point dryer with liquid CO2.
?. Coating and observation: The dehydrated specimen was coated with gold-palladium in an Eiko Model IB5 ion coater (Eiko Engineering Company, Ibaraki, Japan) and observed in a Hitachi Model TM-1000 scanning electron microscope (Tokyo, Japan).
TITLE: Visualizing lower urinary tract afferent projections in the lumbosacral spinal cord in rats
AUTHORS: John-Paul  Fuller-Jackson, Peregrine B Osborne, Janet R Keast

[DESCRIPTION]
This collection includes the protocols required to map the lower urinary tract afferent projections to the lumbosacral spinal cord of male and female Sprague-Dawley rats. Afferents can be visualized using 3D reconstruction of alternating sections in TissueMaker (MBF Bioscience), or through the immunolabelling and clearing method, iDISCO. The following protocols are performed, regardless of endpoint:

STAGE 1: Use of cholera toxin subunit B to label neural projections to lower urinary tract organs.

STAGE 2: Intracardiac perfusion with fixative for anatomical studies.

The next set of protocols pertain to the 3D reconstruction of spinal cord from alternating sections.

STAGE 3: Immunohistochemical labelling of lower urinary tract afferents in spinal cord.

STAGE 4: Quantitation of lower urinary tract afferents in 3D reconstruction of lumbosacral spinal cord sections

For the visualization of lower urinary tract afferents in the intact spinal cord, skip Stages 3 and 4, and instead use Stage 5:

STAGE 5: Immunolabelling and clearing of intact spinal cord for visualization of lower urinary tract afferents

[STEPS]
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.</p>

[STEPS]
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