TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This protocol describes the preparation of Illumina libraries for high through-put sequencing of full-length SSU rRNA sequences from all domains of life without primer bias.</p>
<p> </p>
<p>Sequences for primers and adaptor used can be found under guidelines.</p>

[GUIDELINES]
<p><strong>Primers and adaptors used for library preparation:</strong></p>
<table width="1068">
<tbody>
<tr>
<td width="154">Oligo name</td>
<td width="840">sequence</td>
<td width="74">Purification</td>
</tr>
<tr>
<td>SSU_rRNA_RT1</td>
<td>AGGGGGGCAAAGATGAAGATNNNNNNNNNNNNNNNTCGCCTTAACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTVN</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_RT2</td>
<td>AGGGGGGCAAAGATGAAGATNNNNNNNNNNNNNNNCTAGTACGACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTVN</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_RT3</td>
<td>AGGGGGGCAAAGATGAAGATNNNNNNNNNNNNNNNTTCTGCCTACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTVN</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_RT4</td>
<td>AGGGGGGCAAAGATGAAGATNNNNNNNNNNNNNNNGCTCAGGAACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTVN</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_RT5</td>
<td>AGGGGGGCAAAGATGAAGATNNNNNNNNNNNNNNNAGGAGTCCACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTVN</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_RT6</td>
<td>AGGGGGGCAAAGATGAAGATNNNNNNNNNNNNNNNCATGCCTAACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTVN</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_RT7</td>
<td>AGGGGGGCAAAGATGAAGATNNNNNNNNNNNNNNNGTAGAGAGACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTVN</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_RT8</td>
<td>AGGGGGGCAAAGATGAAGATNNNNNNNNNNNNNNNCCTCTCTGACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTVN</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_RT9</td>
<td>AGGGGGGCAAAGATGAAGATNNNNNNNNNNNNNNNAGCGTAGCACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTVN</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_RT10</td>
<td>AGGGGGGCAAAGATGAAGATNNNNNNNNNNNNNNNCAGCCTCGACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTVN</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_s </td>
<td>/5Phos/GGGCAATATCAGCACCAACAGAAA/3SpC3/</td>
<td>HPLC</td>
</tr>
<tr>
<td>SSU_rRNA_l</td>
<td>CTCCACCCAGACTCATCCATNNNNNNNNNNNNNNNATAGAGGCTTTCTGTTGGTGCTGATATTGC</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_pcr_rv </td>
<td>AGGGGGGCAAAGATGAAGAT</td>
<td>HPLC</td>
</tr>
<tr>
<td>SSU_rRNA_pcr_fw</td>
<td>CTCCACCCAGACTCATCCAT</td>
<td>HPLC</td>
</tr>
<tr>
<td>SSU_rRNA_readtag_rv </td>
<td>CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTAGGGGGGCAAAGATGAAGAT</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_readtag_fw</td>
<td>CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTCCACCCAGACTCATCCAT</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_linktag_rv </td>
<td>CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTGCAATATCAGCACCAACAGAAA</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_linktag_fw</td>
<td>AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGAAGATAGAGCGACAGGCAAGT</td>
<td>PAGE</td>
</tr>
<tr>
<td>SSU_rRNA_read2_rv</td>
<td>GCTCTTCCGATCTAGGGGGGCAAAGATGAAGAT </td>
<td>HPLC</td>
</tr>
<tr>
<td>SSU_rRNA_read2_fw</td>
<td>GCTCTTCCGATCTCTCCACCCAGACTCATCCAT </td>
<td>HPLC</td>
</tr>
</tbody>
</table>
<p> </p>

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