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[DESCRIPTION]
<p> </p>
<p> </p>
<p>The OmniPrep™ kit isolates high quality genomic DNA from many different species and tissue types including animal, plant, bacteria, yeast, fungi, whole blood, and cells in culture. DNA can be isolated from samples high in polysaccharides or other contaminants that are difficult to remove from the DNA preparations.</p>
<p> </p>
<p>This protocol is for use with <strong>body fluids (including CSF, plasma, saliva, serum, sputum, synovial fluid, urine and whole blood)</strong>. Please refer to the <a href="https://www.protocols.io/view/OmniPrep-Protocol-Collection-for-High-Quality-Geno-e7rbhm6" target="_blank">appropriate protocol</a> depending on your application.</p>

[BEFORE_START]
<p><em>Proteinase K Solution</em>: To avoid repeated freezing-thaw, dispense the Proteinase K solution into aliquots of 30µl/tube and freeze at -20°C.</p>
<p> </p>
<p><em>Genomic Lysis Buffer &amp; DNA Stripping Solution</em>: If a precipitate forms due to cold storage allow to warm to room temperature until precipitate dissolves.</p>

[GUIDELINES]
<p><strong>INTRODUCTION</strong></p>
<p>The OmniPrep™ kit isolates high quality genomic DNA from many different species and tissue types including animal, plant, bacteria, yeast, fungi, whole blood, and cells in culture. DNA can be isolated from samples high in polysaccharides or other contaminants that are difficult to remove from the DNA preparations.</p>
<p> </p>
<p>Several unique features separate the OmniPrep™ kit from other methods:</p>
<p>• A unique and proprietary formulation of detergents and salts. DNA can be extracted and purified from almost any tissue without the use of toxic agents such as phenol.</p>
<p>• A quick protocol, which isolates extremely clean genomic DNA. On an average, the DNA is 100kb in size and generally hydrates in minutes.</p>
<p>• Provides an option to modify protocol for difficult to handle samples.</p>
<p>• Suitable for 200 Preps (10mg/prep). The kit is adaptable for larger tissue volumes.</p>
<p> </p>
<p><strong>ITEM(S) SUPPLIED</strong></p>
<table style="width: 394px; height: 414px;">
<tbody>
<tr>
<td style="width: 127.102px;"><strong>Description</strong></td>
<td style="width: 114.375px;"><strong>Cat. # 786-136</strong></td>
<td style="width: 131.648px;"><strong>Cat. # 786-136S </strong></td>
</tr>
<tr>
<td style="width: 127.102px;">Genomic Lysis Buffer</td>
<td style="width: 114.375px;">100ml</td>
<td style="width: 131.648px;">2 x 2ml</td>
</tr>
<tr>
<td style="width: 127.102px;">Nuclei Isolation Buffer</td>
<td style="width: 114.375px;">2 x 30ml</td>
<td style="width: 131.648px;">-</td>
</tr>
<tr>
<td style="width: 127.102px;">DNA Stripping Solution</td>
<td style="width: 114.375px;">10ml</td>
<td style="width: 131.648px;">0.5ml</td>
</tr>
<tr>
<td style="width: 127.102px;">Precipitation Solution</td>
<td style="width: 114.375px;">30ml</td>
<td style="width: 131.648px;">2ml</td>
</tr>
<tr>
<td style="width: 127.102px;">Mussel Glycogen (10mg/ml)</td>
<td style="width: 114.375px;">1ml</td>
<td style="width: 131.648px;">25µl</td>
</tr>
<tr>
<td style="width: 127.102px;">TE Buffer</td>
<td style="width: 114.375px;">20ml</td>
<td style="width: 131.648px;">0.5ml</td>
</tr>
<tr>
<td style="width: 127.102px;">Longlife™ RNase (5mg/ml; &gt;60U/mg)</td>
<td style="width: 114.375px;">0.5ml</td>
<td style="width: 131.648px;">50µl</td>
</tr>
<tr>
<td style="width: 127.102px;">Longlife™ Proteinase K (5mg/ml)</td>
<td style="width: 114.375px;">2 x 0.5ml</td>
<td style="width: 131.648px;"> 50µl</td>
</tr>
</tbody>
</table>
<p><em><strong>NOTE:</strong></em> Cat. # 786-136S is a trial/sample size and does not contain enough reagents for all the protocols. For regular size kit, order Cat. # 786-136.</p>
<p> </p>
<p><strong>STORAGE CONDITIONS</strong></p>
<p>The kit is shipped at ambient temperature. Upon arrival, store the kit components as recommended on the reagent label.</p>
<p> </p>
<p><strong>ADDITIONAL REAGENTS REQUIRED</strong></p>
<p>• For all samples: Isopropanol, 70% Ethanol, and Chloroform</p>
<p>• For Gram positive Bacteria: Lysozyme, 0.5M EDTA. Longlife™ Lysozyme, (Cat# 786-037) available.</p>
<p>• For Yeast: Zymolyase® , β-mercaptoethanol, phosphate buffered saline (PBS). Longlife™ Zymolyase® (Cat# 786-036) available.</p>
<p> </p>
<p><strong>PREPARATION BEFORE USE</strong></p>
<p><em>Proteinase K Solution</em>: To avoid repeated freezing-thaw, dispense the Proteinase K solution into aliquots of 30µl/tube and freeze at -20°C.</p>
<p> </p>
<p><em>Genomic Lysis Buffer &amp; DNA Stripping Solution</em>: If a precipitate forms due to cold storage allow to warm to room temperature until precipitate dissolves.</p>
<p> </p>
<p><strong>PROTOCOLS </strong></p>
<p>The protocols contained in this manual are listed below and are based on the Extraction from Solid Tissue protocol. Thoroughly review the appropriate protocols before commencing isolation of genomic DNA. </p>
<p> </p>
<p><strong>TROUBLESHOOTING</strong></p>
<p><strong>• POOR DNA RECOVERY:</strong></p>
<p>o Increase volumes of Genomic Lysis Buffer, DNA Stripping Solution and Precipitation Solution proportionally.</p>
<p>o Introduce optimal Proteinase K step after step 4.</p>
<p>o Improve grinding technique. </p>
<p>• For efficient grinding of small samples we offer Molecular Grinding Resin™ (Cat. # 786-138), high tensile micro-particles that do not bind nucleic acids and are recommended for grinding and isolation of genomic DNA. </p>
<p>• DNA/RNA free matching pestles and microfuge tubes (1.5ml) for grinding small samples are also available (Cat. # 786-138P).</p>
<p> </p>
<p><strong>• FOR &gt;100kb GENOMIC DNA</strong></p>
<p>o Recommend using MegaLong™ DNA isolation kit (Cat. # 786-146, 786-147). Isolates nuclei under mild condition, which are then transferred to Tube-ODIALYZER™ , a new device for DNA isolation. Nuclei are digested with protease followed by dialysis to remove protein and other contaminants. For more information call our Technical Department.</p>
<p> </p>
<p><strong>Typical Yield:</strong> 0.2-5µg DNA/100µl body fluid.</p>
<p> </p>
<p><strong>CITATIONS</strong></p>
<p>1. Staley, C.A. et al (2012) Gene. 496:118</p>
<p>2. Jonkers, W. et al (2012) Appl Envir Microbiol 78:3656</p>
<p>3. Li, Z. et al (2010) Protein Expression and Purification. 72:113</p>
<p>4. Li, Z. et al (2010) Biochem and Biophys Res Comm. 402:519</p>
<p>5. Lorch, J. et al (2010) J Vet Diagn Invest 22:224</p>
<p>6. Li, Z. et al (2009) Protein Expression and Purification. 67:175</p>
<p>7. Lin-Cereghino, J. et al (2008) Yeast. 25:293</p>
<p>8. Choi, Y. and Shim, W. (2008) Microbiology. 154: 326</p>
<p>9. Choi, Y. et al (2008) Mycologia. 100:701</p>
<p>10. Whitaker, V. et al (2007) J. Amer. Soc. Hort. Sci. 132:534</p>
<p>11. Szabo, L. J. (2007) Mol Ecol Notes 7:92</p>
<p>12. Ordonez, M.E. and Kolmer, J.A. (2007) Phytopathology 97:574</p>
<p>13. Sagaram, U.S. et al (2006) Mol Plant Pathol 7:381</p>
<p>14. Mertens, J.A. et al (2006) Arch. Microbiol. 186:41</p>
<p>15. Lee, B et al (2005) Genome. 48:1104</p>
<p>16. Pliss, L. et al (2004) J. Neurochem. 91:1082</p>
<p>17. Jacobs-Helber, S. et al (2002) JBC. 277:4859</p>
<p>18. Li, X. et al (2002) Genome. 45:229</p>
<p>19. Villar, M. et al (2001) J. Bact. 183:55</p>

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