TITLE: No Title
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[BEFORE_START]
Prepare 5 μM primer solutions.
 
 

[GUIDELINES]
Perform reactions in small batches until you are confident that there is no cross-contamination among the samples. Including isolation blanks and PCR blanks is crucial for the quality control.

ITS2 primers used in the 1st PCR:

ITS2-4R GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNTCCTCCGCTTATTGATATGC
ITS2-S2F ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNATGCGATACTTGGTGTGAAT

Indexing primers used in the 2nd PCR (xxxxxxxx - index):

AATGATACGGCGACCACCGAGATCTACACxxxxxxxxACACTCTTTCCCTACACGACGC
CAAGCAGAAGACGGCATACGAGATxxxxxxxxGTGACTGGAGTTCAGACGTGTGC

PCR_F_A501:	AATGATACGGCGACCACCGAGATCTACACTGAACCTTACACTCTTTCCCTACACGACGC
PCR_F_A502:	AATGATACGGCGACCACCGAGATCTACACTGCTAAGTACACTCTTTCCCTACACGACGC
PCR_F_A503:	AATGATACGGCGACCACCGAGATCTACACTGTTCTCTACACTCTTTCCCTACACGACGC
PCR_F_A504:	AATGATACGGCGACCACCGAGATCTACACTAAGACACACACTCTTTCCCTACACGACGC
PCR_F_A505:	AATGATACGGCGACCACCGAGATCTACACCTAATCGAACACTCTTTCCCTACACGACGC
PCR_F_A506:	AATGATACGGCGACCACCGAGATCTACACCTAGAACAACACTCTTTCCCTACACGACGC
PCR_F_A507:	AATGATACGGCGACCACCGAGATCTACACTAAGTTCCACACTCTTTCCCTACACGACGC
PCR_F_A508:	AATGATACGGCGACCACCGAGATCTACACTAGACCTAACACTCTTTCCCTACACGACGC
PCR_F_D501:	AATGATACGGCGACCACCGAGATCTACACTATAGCCTACACTCTTTCCCTACACGACGC

PCR_F_D502:	AATGATACGGCGACCACCGAGATCTACACATAGAGGCACACTCTTTCCCTACACGACGC
PCR_F_D503:	AATGATACGGCGACCACCGAGATCTACACCCTATCCTACACTCTTTCCCTACACGACGC
PCR_F_D504:	AATGATACGGCGACCACCGAGATCTACACGGCTCTGAACACTCTTTCCCTACACGACGC
PCR_F_D505:	AATGATACGGCGACCACCGAGATCTACACAGGCGAAGACACTCTTTCCCTACACGACGC
PCR_F_D506:	AATGATACGGCGACCACCGAGATCTACACTAATCTTAACACTCTTTCCCTACACGACGC
PCR_F_D507:	AATGATACGGCGACCACCGAGATCTACACCAGGACGTACACTCTTTCCCTACACGACGC
PCR_F_D508:	AATGATACGGCGACCACCGAGATCTACACGTACTGACACACTCTTTCCCTACACGACGC

PCR_R_A701:	CAAGCAGAAGACGGCATACGAGATGTCGTGATGTGACTGGAGTTCAGACGTGTGC
PCR_R_A702:	CAAGCAGAAGACGGCATACGAGATACCACTGTGTGACTGGAGTTCAGACGTGTGC
PCR_R_A703:	CAAGCAGAAGACGGCATACGAGATTGGATCTGGTGACTGGAGTTCAGACGTGTGC
PCR_R_A704:	CAAGCAGAAGACGGCATACGAGATCCGTTTGTGTGACTGGAGTTCAGACGTGTGC
PCR_R_A705:	CAAGCAGAAGACGGCATACGAGATTGCTGGGTGTGACTGGAGTTCAGACGTGTGC
PCR_R_A706:	CAAGCAGAAGACGGCATACGAGATGAGGGGTTGTGACTGGAGTTCAGACGTGTGC
PCR_R_A707:	CAAGCAGAAGACGGCATACGAGATAGGTTGGGGTGACTGGAGTTCAGACGTGTGC
PCR_R_A708:	CAAGCAGAAGACGGCATACGAGATGTGTGGTGGTGACTGGAGTTCAGACGTGTGC
PCR_R_A709:	CAAGCAGAAGACGGCATACGAGATTGGGTTTCGTGACTGGAGTTCAGACGTGTGC
PCR_R_A710:	CAAGCAGAAGACGGCATACGAGATTGGTCACAGTGACTGGAGTTCAGACGTGTGC
PCR_R_A711:	CAAGCAGAAGACGGCATACGAGATTTGACCCTGTGACTGGAGTTCAGACGTGTGC
PCR_R_A712:	CAAGCAGAAGACGGCATACGAGATCCACTCCTGTGACTGGAGTTCAGACGTGTGC

PCR_R_D701:	CAAGCAGAAGACGGCATACGAGATCGAGTAATGTGACTGGAGTTCAGACGTGTGC
PCR_R_D702:	CAAGCAGAAGACGGCATACGAGATTCTCCGGAGTGACTGGAGTTCAGACGTGTGC
PCR_R_D703:	CAAGCAGAAGACGGCATACGAGATAATGAGCGGTGACTGGAGTTCAGACGTGTGC
PCR_R_D704:	CAAGCAGAAGACGGCATACGAGATGGAATCTCGTGACTGGAGTTCAGACGTGTGC
PCR_R_D705:	CAAGCAGAAGACGGCATACGAGATTTCTGAATGTGACTGGAGTTCAGACGTGTGC
PCR_R_D706:	CAAGCAGAAGACGGCATACGAGATACGAATTCGTGACTGGAGTTCAGACGTGTGC
PCR_R_D707:	CAAGCAGAAGACGGCATACGAGATAGCTTCAGGTGACTGGAGTTCAGACGTGTGC
PCR_R_D708:	CAAGCAGAAGACGGCATACGAGATGCGCATTAGTGACTGGAGTTCAGACGTGTGC
PCR_R_D709:	CAAGCAGAAGACGGCATACGAGATCATAGCCGGTGACTGGAGTTCAGACGTGTGC
PCR_R_D710:	CAAGCAGAAGACGGCATACGAGATTTCGCGGAGTGACTGGAGTTCAGACGTGTGC
PCR_R_D711:	CAAGCAGAAGACGGCATACGAGATGCGCGAGAGTGACTGGAGTTCAGACGTGTGC
PCR_R_D712:	CAAGCAGAAGACGGCATACGAGATCTATCGCTGTGACTGGAGTTCAGACGTGTGC

[STEPS]
SECTION: 1st PCR
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SECTION: 2nd PCR
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SECTION: Purification
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SECTION: 2nd PCR
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SECTION: 2nd PCR
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SECTION: Pooling
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SECTION: Pooling
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SECTION: Pooling
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SECTION: 1st PCR
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SECTION: 1st PCR
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SECTION: 2nd PCR
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SECTION: 2nd PCR
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