TITLE: ASW+NO3 medium
AUTHORS: Roscoff Culture Collection

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Medium used for cyanobacteria based on artificial seawater (ASW)</div></div>

[STEPS]
?. Dissolve 25 g of NaCl in MilliQ waterTo this solution, add  :     Quantity
    
 Compound
    
 Stock Solution
   Concentration in medium (in mM)
     mLstock
 /LASW
   g/LASW
     10
   0,75
   Sodium nitrate (NaNO3)
   75 g/L
   8.8
     10
   2
   Magnesium chloride hexahydrate (MgCl26H2O)
   200 g/L
   9.8
     5
   0,5
   Potassium chloride (KCl)
   100 g/L
   6,7
     5
   0,5
   Calcium chloride (CaCl2)
   100 g/L
   4.5
     10
   3,5
   Magnesium sulfate heptahydrate (MgSO47H2O)
   350 g/L
   14.2
     5,5
   1,1
   TRIS-Base
   200 g/L
   9.08
      
 2,5
    
 0,03
   Dipotassium phosphate (K2HPO4)
    
 12 g/L
    
 0.172
     Adjust the pH to 8 with concentrated HClAdjust to 999 mL with milliQ waterAdd 1 mL of trace metals (see receipe below)Autoclave the medium  ABCDE1Quantity
&nbsp;
Compound
&nbsp;
Stock Solution
Concentration in medium (in mM)
2mLstock
/LASW
g/LASW
310
0,75
Sodium nitrate (NaNO3)
75 g/L
8.8
410
2
Magnesium chloride hexahydrate (MgCl26H2O)
200 g/L
9.8
55
0,5
Potassium chloride (KCl)
100 g/L
6,7
65
0,5
Calcium chloride (CaCl2)
100 g/L
4.5
710
3,5
Magnesium sulfate heptahydrate (MgSO47H2O)
350 g/L
14.2
85,5
1,1
TRIS-Base
200 g/L
9.08
9&nbsp;
2,5
&nbsp;
0,03
Dipotassium phosphate (K2HPO4)
&nbsp;
12 g/L
&nbsp;
0.172
Adjust the pH to 8 with concentrated HClAdjust to 999 mL with milliQ waterAdd 1 mL of trace metals (see receipe below)Autoclave the medium
ABCDE1Quantity
&nbsp;
Compound
&nbsp;
Stock Solution
Concentration in medium (in mM)
2mLstock
/LASW
g/LASW
310
0,75
Sodium nitrate (NaNO3)
75 g/L
8.8
410
2
Magnesium chloride hexahydrate (MgCl26H2O)
200 g/L
9.8
55
0,5
Potassium chloride (KCl)
100 g/L
6,7
65
0,5
Calcium chloride (CaCl2)
100 g/L
4.5
710
3,5
Magnesium sulfate heptahydrate (MgSO47H2O)
350 g/L
14.2
85,5
1,1
TRIS-Base
200 g/L
9.08
9&nbsp;
2,5
&nbsp;
0,03
Dipotassium phosphate (K2HPO4)
&nbsp;
12 g/L
&nbsp;
0.172
?. [Trace metal stock solution]
Dissolve all these components separately in milliQ water :     Quantity
   Compound
     2.86g
   Boric acid (H3BO3)
     1.81g
   Manganese (II) chloride tetrahydrate (MnCl2-4H2O)
     0.222g
   Zinc sulfate monohydrate (ZnSO4-H2O)
     0.390g
   Sodium molybdate dihydrate (Na2MoO4-2H2O)
     0.008g
   Copper sulfate pentahydrate (CuSO4-5H2O)
     0.0494g
   Cobalt nitrate hexahydrate (Co(NO3)2-6H2O)
     3.0g
   Ferric chloride hexahydrate (FeCl-6H2O)
     0.5g
   EDTA magnesium disodium (EDTA(Na2Mg))
    Combine the various solutions after full dissolutionMake final volume up to 1L with milliQStore in refrigerator  AB1Quantity
Compound
22.86g
Boric acid (H3BO3)
31.81g
Manganese (II) chloride tetrahydrate (MnCl2-4H2O)
40.222g
Zinc sulfate monohydrate (ZnSO4-H2O)
50.390g
Sodium molybdate dihydrate (Na2MoO4-2H2O)
60.008g
Copper sulfate pentahydrate (CuSO4-5H2O)
70.0494g
Cobalt nitrate hexahydrate (Co(NO3)2-6H2O)
83.0g
Ferric chloride hexahydrate (FeCl-6H2O)
90.5g
EDTA magnesium disodium (EDTA(Na2Mg))
Combine the various solutions after full dissolutionMake final volume up to 1L with milliQStore in refrigerator
AB1Quantity
Compound
22.86g
Boric acid (H3BO3)
31.81g
Manganese (II) chloride tetrahydrate (MnCl2-4H2O)
40.222g
Zinc sulfate monohydrate (ZnSO4-H2O)
50.390g
Sodium molybdate dihydrate (Na2MoO4-2H2O)
60.008g
Copper sulfate pentahydrate (CuSO4-5H2O)
70.0494g
Cobalt nitrate hexahydrate (Co(NO3)2-6H2O)
83.0g
Ferric chloride hexahydrate (FeCl-6H2O)
90.5g
EDTA magnesium disodium (EDTA(Na2Mg))