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[DESCRIPTION]
<p>This is a protocol for the Isolation of Genomic DNA from Fresh Blood, Bone Marrow &amp; Buffy Coat (5ml blood). </p>
<p>If the buffy coat preparation was processed from 5ml whole blood then follow the Protocol for 5ml Blood.</p>
<p> </p>
<p>(Cat. # <a href="http://www.gbiosciences.com/ResearchProducts/XIT_Blood.aspx" target="_blank">786‐294, 786‐295, 786‐296</a>)</p>

[BEFORE_START]
<p>Preheat a water‐bath or heating block to 55°C and equilibrate TE Buffer to 50‐60°C.</p>

[GUIDELINES]
<p><strong>INTRODUCTION</strong></p>
<p>The XIT™ Genomic DNA Blood kits are designed for the isolation of genomic DNA from whole blood, bone marrow and buffy coat. The XIT™ kit uses the principle of cell lysis, protein precipitation and finally DNA precipitation to isolate high quality genomic DNA.   </p>
<p> </p>
<p>XIT™ Genomic DNA Blood Kit protocol is designed to use 0.5ml whole blood, however the protocol can be easily adapted for larger tissue sample sizes. The purified DNA has an A260/A280 ratio between 1.8 ‐2.0 and has yields ranging between 10‐15µg/ml depending on volume of blood.</p>
<p> </p>
<p><strong>ITEM(S) SUPPLIED</strong></p>
<table style="width: 573px; height: 205px;">
<tbody>
<tr>
<td style="width: 201px;"><strong>Description</strong></td>
<td style="width: 118px;">
<p><strong>Cat # 786‐294</strong></p>
<p>≤12.5ml blood</p>
</td>
<td style="width: 127px;">
<p><strong>Cat # 786‐295</strong></p>
<p><strong> </strong>≤125ml blood</p>
</td>
<td style="width: 123px;">
<p><strong>Cat # 786‐296 </strong></p>
<p>≤250ml blood</p>
</td>
</tr>
<tr>
<td style="width: 201px;"> RBC Lysis Buffer</td>
<td style="width: 118px;">         100ml</td>
<td style="width: 127px;">     2 x 250ml</td>
<td style="width: 123px;">     4 x 250ml</td>
</tr>
<tr>
<td style="width: 201px;"> XIT™ Lysis Buffer</td>
<td style="width: 118px;">          10ml    </td>
<td style="width: 127px;">     100ml</td>
<td style="width: 123px;">     2 x 100ml </td>
</tr>
<tr>
<td style="width: 201px;"> XIT™ Protein Precipitation Buffer</td>
<td style="width: 118px;">          2.5ml</td>
<td style="width: 127px;">      25ml</td>
<td style="width: 123px;">     2 x 25ml</td>
</tr>
<tr>
<td style="width: 201px;"> TE Buffer</td>
<td style="width: 118px;">          1.5ml</td>
<td style="width: 127px;">      20ml</td>
<td style="width: 123px;">     2 x 20ml</td>
</tr>
<tr>
<td style="width: 201px;"> LongLife™ RNase</td>
<td style="width: 118px;">          0.5ml   </td>
<td style="width: 127px;">       0.5ml</td>
<td style="width: 123px;">     2 x 0.5ml</td>
</tr>
</tbody>
</table>
<p> </p>
<p> </p>
<p><strong>STORAGE CONDITIONS</strong></p>
<p>The kit is shipped at ambient temperature. Upon arrival, store the LongLife™ RNase at ‐20°C and all other kit components at room temperature. The kit components are stable for 1 year, if stored properly.</p>
<p> </p>
<p><strong>ADDITIONAL ITEMS REQUIRED</strong></p>
<p>• Isopropanol</p>
<p>• 70% ethanol  </p>
<p> </p>
<p><strong> BUFFY COATS &amp; BONE MARROW SAMPLES</strong></p>
<ul>
<li>For processing buffy coats, use the volumes required for processing the original blood sample.  For example, if the buffy coat preparation was processed from 5ml whole blood then follow the Protocol for 5ml Blood.</li>
<li>For bone marrow samples, ensure that the sample is completely homogenous after addition of XIT™ Lysis Buffer.  If not add additional XIT™ Lysis Buffer until an homogenous sample is obtained.</li>
</ul>
<p> </p>

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