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AUTHORS: 

[BEFORE_START]
<ol>
<li>Clean all surfaces and pipettors to remove DNA</li>
<li>Label Plates
<ul>
<li>Plate #1 – 1ml collection plate</li>
<li>Plate #2 – 1ml collection plate</li>
<li>Plate #3 – 1ml collection plate</li>
<li>Plate #4 – 1ml collection plate</li>
<li>Plate #5 – 2ml collection plate</li>
<li>Plate #6 – 2ml collection plate</li>
</ul>
</li>
<li>UV Sterilize and Label reservoirs
<ul>
<li>Bead solution – 750ul/well</li>
<li>C1 – 60ul/well</li>
<li>C2 – 250ul/well</li>
<li>C3 – 200ul/well</li>
<li>C4 – 650ul/well</li>
<li>C5 – 500ul/well</li>
<li>C6 – 100ul/well</li>
</ul>
</li>
</ol>

[GUIDELINES]
<p><strong><a href="https://mobio.com/media/wysiwyg/pdfs/protocols/27000-4-KF.pdf" target="_blank" rel="noopener noreferrer">MoBio PowerMag Soil DNA Isolation Kit (Optimized for KingFisher)</a></strong></p>
<p> </p>
<p>The Knight lab has transitioned to the PowerMag Soil DNA Isolation Kit (Optimized for KingFisher). We have validated a variety of sample types to ensure reproducibility when compared to MoBio PowerSoil Extraction Kit. This transition occurred to increase efficiency and reduce DNA extraction time from 6-8 hours to 2.5-3 hours.</p>
<p> </p>
<p>The protocol is followed as MoBio recommends, with an added 10-minute water bath at 65°C after step 7.</p>
<p> </p>
<p>The new kit can be implemented on the epMotion using a magnetic bead plate adapter for the epMotion. However, it does not reduce the amount of time the extraction takes by a significant amount: tests showed that the new kit on the epMotion took longer than the old kit. Comparison showed both the old and new kit performed well on the epMotion.</p>
<p> </p>
<p><a href="https://mobio.com/products/dna-isolation/soil/powersoilr-htp-96-well-soil-dna-isolation-kit.html" target="_blank" rel="noopener noreferrer"><strong>PowerSoil-htp 96 Well Soil DNA Isolation Kit</strong></a></p>
<p> </p>
<p>The MoBio PowerSoil DNA Isolation Kit is still compatible if KingFisher instrumentation is not available.</p>
<p> </p>
<p><strong>Items included in the extraction kit (for 1-96 well extraction)</strong></p>
<ul>
<li>(1) Bead plate</li>
<li>(1) Spin plate (filter)</li>
<li>(1) 0.5ml collection</li>
<li>(4) 1.0ml collection plates</li>
<li>(2) 2.0ml collection plates</li>
<li>(1) Microplate (DNA elution)</li>
<li>Sealing Tape</li>
<li>Centrifuge Tape</li>
<li>Elution Sealing Mat</li>
<li>Labeled solutions</li>
</ul>
<p> </p>
<p><strong>Important Considerations </strong></p>
<p> </p>
<ol>
<li>Normal diameter 1 ml pipet tips are too large for some of the pipetting steps. To get around this problem we use a Rainin 300 ul 8-channel with filtered tips (Rainin #SR-L300F) and a Rainin 1000 ul 8-channel with extended length filtered tips (Rainin #RTS-L1000XF).</li>
<li>Make sure that all of the necessary consumables and reagents are in place before you start the extraction. Remember, each pipetting step will require 1 box of 96 tips per plate.</li>
<li>We use individually wrapped reagent reservoirs and expose them to UV light for 30 minutes prior to usage.</li>
<li>In Step 10, it is important that the plate not rub against any surfaces in the shaker.</li>
<li>Make sure that the alpha-numeric grid is in the same orientation across all the plates. On a few occasions we have noticed that the sticker is not always in the same position on the plate.</li>
</ol>
<p><br /><strong>Next Steps</strong></p>
<ul>
<li><a href="https://www.protocols.io/view/emp-16s-rrna-amplification-protocol-nuudeww" target="_blank" rel="noopener noreferrer">16S rRNA Amplification Protocol</a></li>
<li><a href="https://www.protocols.io/view/emp-18s-rrna-amplification-protocol-nuvdew6?step=6" target="_blank" rel="noopener noreferrer">18S rRNA Amplification Protocol</a></li>
</ul>

[STEPS]
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