TITLE: Protocol 1: Micropipette
AUTHORS:  

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>For this lab, you will understand the </span><span style = ":UNDERLINE;">mechanics</span><span>, know how t</span><span style = ":UNDERLINE;">o use the buttons</span><span>,  and understand the </span><span style = ":UNDERLINE;">standard error</span><span> involved with a micropipette.</span></div><div class = "text-block"><span>Students must know how to appropriately</span><span style = ":UNDERLINE;"> use </span><span>their tools, it is vital that they understand how to use their micropipette for they will have to use it for the remainder of their lab course/career. They should understand when to use which tip and how to extract and give precise measurements of the product</span></div><div class = "text-block"><span>Students will understand how there is </span><span style = ":UNDERLINE;">error involved </span><span>in micropipettes. Every time they use it there will be different results. This understanding of error also allows us and them to know that their equipment is accurately calibrated/assembled. </span></div></div>

[STEPS]
?. [Using the Micropipette]
Hold Micropipette firmly in hand.
?. [Using the Micropipette]
Turn the dial to the respective volume. Make sure you do not turn the dial past the maximum point.
?. [Using the Micropipette]
Now, gently press on the top of the pipette until you reach the first click.Release the the button to fully extract the desired amount of the solution into the tip.Make sure that the pipette is held perfectly veritcal to ensure that the full volume of what the solution you want is extracted.
?. [Using the Micropipette]
Once the solution is in the tip, place the pipette into the empty container you would like to dispense the solution in. Firmly press the top of the pipette to dispense the full volume of the solution into the container.
?. [Using the Micropipette]
When the full volume of the solution is out of the tip, you may place to the tip over the "tip waste" container and discard the used tip by pressing the eject button.
?. [Calculating Mass]
Turn on your weighing scale (0.000 g)
?. [Calculating Mass]
Once the scale is on, a number will display. Add the weigh boats and hit the tare button.The tare will calibrate whatever is on the scale to display as zero. The scale should display no weight is on it, even though our weigh boat is on top of it.
?. [Calculating Mass]
Using the steps in the above section (Using the micropipette),  pipette a known volume of your reagent (water or glycerol).
?. [Calculating Mass]
Add reagent to weigh boat and record the measured weight.
?. [Calculating Mass]
After recording the weight, hit the tar button
?. [Calculating Mass]
Repeats steps 8-11 for 5 times with each reagent and each micropipette.
?. [Calculating Mass]
Discard the weigh boat with the reagents on it.
?. [Calibrating your Scale]
Turn the scale on and wait for the display to show “0.000g”
?. [Calibrating your Scale]
Press and hold the ON key until the display shows “CAL” then release
?. [Calibrating your Scale]
Press the ON key once more. The display will flash 10.000g.
?. [Calibrating your Scale]
Place one of the 10g calibration weights on the center of the platform. Once the weight is accepted, the display will change to show 20.000g
?. [Calibrating your Scale]
Place the second 10g weight on the top of the first one to equal a total of 20.000g. The scale is then calibrated.
?. [Using the Micropipette]
Add the correct pipette tip for the appropiate volume. Large tips for  . Small tips for   and .
1000 µl
200 µl
20 µl