eamag/protocols-with-steps huggingface dataset:
TITLE: Collecting Citations from Text
AUTHORS: Rebecca Hedreen

[DESCRIPTION]
Basic steps and scripts used for translating text citations to bibtex files suitable for loading into citation management software or citation analysis scripts. 4 publically available webpage mounted scripts are suggested, that require no programming.

Updated in April 2023 to include a ChatGPT option

[STEPS]
SECTION: Preparing text file
1. Copy citations from the source document(s) into a text (.txt) document.
 

2. Edit the text document so that each citation is on a separate line with one blank line between each citation. Not all the scripts require a blank line between citations, but it does improve readability and importing.
 
 

SECTION: Processing citations
3. I've found 4 web-mounted scripts that will do text file to bibtex file translation. Only one is needed to produce a bib file - different text citation formats may work better in different systems, however.
SECTION: Export
4. Once the script has exported a .bib file, load it into your citation manager of choice. Zotero has been the most reliable for importing. Zotero also has an option to "Import from Clipboard" if the output is available for copy & paste.
 

 

SECTION: Processing citations
3.1. Anystyle.io can be used on the web, or as a Ruby script. This is the most flexible script and it allows detailed editing before the final file export. The website is privately hosted, so it's not always been available. https://anystyle.io/
 

 

SECTION: Processing citations
3.2. This PERL script is mounted on a website and will do basic translation. No support, but it's quite good. http://www.snowelm.com/~t/doc/tips/makebib.perl.cgi
 

 

SECTION: Processing citations
3.3. Hosted by the University of Toronto. Requires registration. Basic editing and checking in Google Scholar is available. Accurate and reliable. https://text2bib.economics.utoronto.ca/

 

 

SECTION: Processing citations
3.4. ChatGPT can take text citations, even with errors or in non-standard formats, and format them as bibtex with high accuracy. While it is not required to process the text as much as for the other services, it may make it easier to be sure you are getting the correct number of fully formatted citations. The following prompt has worked well, but variations should work as well.
TITLE: ATG3 construct cloning
AUTHORS: lmstrong

[DESCRIPTION]
ATG3 cloning 

[STEPS]
SECTION: Ligation Independent Cloning
1. Amplify gene using PCR with Q5 polymerase. Design primers with overhangs compatible with 1GFP and 2BT vectors. For mutants, design overlapping primers in opposing direction as if using around the horn. Use these to perform 2-step PCR, verifying each step via gel. 
SECTION: Ligation Independent Cloning
2. Gel extract correct size band and measure concentration 
SECTION: Ligation Independent Cloning
3. Digest vector with SspI at 37 C for 1 hour.  After 1 hour add CIAP to vector digest and incubate at 37 for another 30 minutes
SECTION: Ligation Independent Cloning
4. Gel extract vector digest. PCR clean up insert digest. Measure concentrations of both. 
SECTION: Ligation Independent Cloning
5. Mix vector and insert at 1:4 molar ratio for ligation with 1 µL NEBuffer 2.1 and 1 µL 100 mM DTT to a final volume of 10 µL. Add 0.3 µL of NEB T4 DNA polymerase, and immediately incubate for 30 seconds at 50°C
SECTION: Ligation Independent Cloning
6. Transform 4uL of reaction into DH5alpha cells. Plate everything. Sequence colonies to ensure correct mutation.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p><strong>Murashige and Skoog medium</strong> (or <em><strong>MSO</strong></em> or <em><strong>MS0</strong></em> <em>(MS-zero)</em>) is a <a href="https://en.wikipedia.org/wiki/Plant" target="_blank">plant</a> <a href="https://en.wikipedia.org/wiki/Growth_medium" target="_blank">growth medium</a> used in the laboratories for cultivation of plant <a href="https://en.wikipedia.org/wiki/Cell_culture" target="_blank">cell culture</a>. MSO was invented by plant scientists <a href="https://en.wikipedia.org/wiki/Toshio_Murashige" target="_blank">Toshio Murashige</a> and <a href="https://en.wikipedia.org/wiki/Folke_K._Skoog" target="_blank">Folke K. Skoog</a> in 1962 during Murashige's search for a new <a href="https://en.wikipedia.org/wiki/Plant_growth_regulator" target="_blank">plant growth regulator</a>. A number behind the letters MS is used to indicate the sucrose concentration of the medium. For example, MS0 contains no sucrose and MS20 contains 20 g/l sucrose. Along with its modifications, it is the most commonly used medium in <a href="https://en.wikipedia.org/wiki/Plant_tissue_culture" target="_blank">plant tissue culture</a> experiments in laboratorium.<sup class="reference"><a href="https://en.wikipedia.org/wiki/Murashige_and_Skoog_medium#cite_note-1" target="_blank">[1]</a></sup></p>
<p> </p>
<p>Source:</p>
<p>http://cshprotocols.cshlp.org/content/2010/2/pdb.rec12142.full?text_only=true</p>
<p> </p>

[GUIDELINES]
<p>Derivatives of MS medium can be made through the addition of sucrose, both 1% (w/v) and 2% (w/v) are commonly used. </p>

[STEPS]
?. 
?. 
?. 
?.
TITLE: Liver Landscape project (HCA) publication link
AUTHORS: Christine Briggs

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">HCA does not provide the Case Selection Protocol. We will provide a link to the project publication for the purposes of providing the required protocol DOI to huBMAP.</div></div>

[STEPS]
?. [HCA_liver_landscape-Case_Selection_Protocol]
This is the DOI to the project publication:https://doi.org/10.1038/s41467-018-06318-7
TITLE: Golden Gate lvl 1/2
AUTHORS: Vinca Seiler, René Inckemann

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Golden Gate reaction protocol for lvl 1/2</div></div>

[STEPS]
?. [Pipetting scheme for assembly reaction]
of each DNA insert. For an improved assembly efficiency, the amount of DNA inserts can optionally be normalized to equimolar concentrations (∼ each) or use  of each insert (antibiotic resistance part should be diluted 1:10, -).
0.5 µl
20
75 ng
7.5 ng
10 ng
?. [Pipetting scheme for assembly reaction]
T4 DNA Ligase buffer (NEB)
1 µl
?. [Pipetting scheme for assembly reaction]
T4 DNA Ligase (NEB)
0.5 µl
?. [Pipetting scheme for assembly reaction]
BsaI-HF®v2(NEB) for  lvl 1 / EspI3 (NEB) for lvl 2
0.5 µl
?. [Pipetting scheme for assembly reaction]
Water to . ( also possible) of each DNA insert
10 µl
20 µl
0.5 µl
?. [Thermocycler Improved Protocol]
37 °C
?. [Thermocycler Improved Protocol]
16 °C
?. [Thermocycler Improved Protocol]
Cycle step 6 and 7 15x
?. [Thermocycler Improved Protocol]
50 °C
?. [Thermocycler Improved Protocol]
80 °C
?. [Alternative Thermocycler Troubleshoot/Overnight Protocol]
37 °C
?. [Alternative Thermocycler Troubleshoot/Overnight Protocol]
16 °C
?. [Alternative Thermocycler Troubleshoot/Overnight Protocol]
Cycle steps 11 & 12 x 50
?. [Alternative Thermocycler Troubleshoot/Overnight Protocol]
50 °C
?. [Alternative Thermocycler Troubleshoot/Overnight Protocol]
80 °C
?. [Transformation]
Add - of each assembly reaction added to  competent cells.
2 µl
5 µl
50 µl
?. [Transformation]
Cells should be recovered for   (Amp) to  (Kan, Chloramphenicol).
TITLE: Thawing of CT-2A cell line
AUTHORS: Guido  Krähenbühl

[DESCRIPTION]
This protocol describes the thawing of mouse CT-2A glioma cells


[BEFORE_START]
Make sure to warm up media to 37 °C 


[GUIDELINES]
GMO safety level 1

[STEPS]
1. Remove cryovial from liquid nitrogen storage and place on dry-ice for transfer to cell culture lab.


 

2. Remove cryovial from dry-ice and hold in a 37 °C  waterbath until  thawed
 

Dry the cryovial thoroughly, spray with 70% ethanol and transfer to cell culture hood
3. Transfer contents of cryovial to a   containing 7 mL .

4. Centrifuge at 300 rcf, 5 min 

5. Discard supernatant, resubstitute with fresh 7 mL
3.1. Rinse the cryovial with 1 mL  of the cell suspension and add it back to the same  

6. Transfer cell suspension to a   and agitate gently back and forth and side to side to evenly distribute cells
7. Place the petri dish in a 37 °C (5%CO2)

8. After 24 hours, inspect adherence and confluency.
TITLE: CDx autoXpress detection of COVID 19
AUTHORS: Mickey Shah, Gary Niehaus

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The Crystal Diagnostics AutoXpress provides rapid detection of COVID 19 at 10 - 100 CFU per ul of clinical sample (nasal swab, sputum, or saliva). The patented Liquid Crystal technology is combined with antibody-coated paramagnetic microspheres to selectively capture and detect COVID 19.</div></div>

[STEPS]
?. [Automated]
Transfer  of test sample to 96 well plate. Each sample will be transferred to two wells, one for POS and one for NEG.
600 µl
?. [Automated]
Mix the magnetic 3um microspheres for POS and NEG using a pipette.
?. [Automated]
Dispense  of the magnetic 3um microspheres to each well.
100 µl
?. [Automated]
Shake the plate at  , followed by .
33
33
?. [Automated]
Transfer the plate to the magnet block and wait for
?. [Automated]
Aspirate all the liquid from each well without disturbing the pellet.
?. [Automated]
Add  of PBS to each well  and wait for
900 µl
?. [Automated]
Aspirate all the liquid from each well without disturbing the pellet.
?. [Automated]
Transfer plate to shaker from the magnet block and shake the plate at
33
?. [Automated]
Mix the 10um poly microspheres using a pipette.
?. [Automated]
Dispense  of the 10um poly microspheres to each well.
100 µl
?. [Automated]
Shake the plate at  , followed by
33
33
?. [Automated]
Transfer the plate to magnet block and wait for
?. [Automated]
Aspirate all the liquid from each well without disturbing the pellet.
?. [Automated]
Dispense  of PBS to each well and wait for
300 µl
?. [Automated]
Aspirate all the liquid from each well without disturbing the pellet.
?. [Automated]
Dispense  of PBS to each well and wait for
300 µl
?. [Automated]
Aspirate  of liquid  from each well without disturbing the pellet.
280 µl
?. [Automated]
Transfer plate to shaker from the magnet block and shake the plate at
33
?. [Automated]
Transfer the plate from shaker to Reader side
?. [Automated]
Mix the sample using a pipette. Transfer  of the sample to  of liquid crystal.
10 µl
60 µl
?. [Automated]
Gently mix the sample with liquid crystal and transfer the mix to BioSlide.
?. [Automated]
To disperse the liquid crystal, apply positive pressure to the BioSlide.
?. [Automated]
After , BioSlide is read using the camera reader. The image and event count is obtained for each sample.
TITLE: RNA stability in SPECTRA
AUTHORS: Venkata Subramanian  Ramesan, Bhushan  Toley

[DESCRIPTION]
Protocol for storage of RNA in SPECTRA.

[STEPS]
SECTION: Source the RNA
1. Obtain any RNA (RNase P, SARS CoV-2, etc.)  
SECTION: Source the RNA
2. Quantify the RNA using a Nanodrop or RT-PCR. Convert to copy number using the following tool.

RNA copy number calculator
SECTION: Apply RNA to the nylon flock
3. Add 10ul of 10^4 copies /ul RNA to the  swab
 


SECTION: Load the swab into the SPECTRA
4. Open the screw cap of the SPECTRA device and place it on a 50ml falcon stand (try to minimize exposure to air by keeping the device opened. Open the SPECTRA device just before loading the swab) 


3.1. Caution: the swab is slightly hydrophobic, hence try to smear the 10ul drop over the nylon flock with a pipette tip
SECTION: Load the swab into the SPECTRA
5. Put the swab into the centrifuge tube present inside the SPECTRA tube, and cut the plastic body just above the nylon flock head
SECTION: Load the swab into the SPECTRA
6. DO NOT CLOSE THE 1.5ml CENTRIFUGE TUBE (IMPORTANT)
SECTION: Load the swab into the SPECTRA
7. Close the 50ml centrifuge tube by screwing it tight 
SECTION: Load the swab into the SPECTRA
8. Store the SPECTRA tube for different durations at room temperature (25°C) 


 

(2 controls, and 3 test)
SECTION: RT-PCR
9. Add  300 µL of nuclease-free water to  1.5 mL centrifuge tube and mix it vigorously using a vortexer for at least 5 seconds each.
 


SECTION: RT-PCR
11. Perform RT-PCR reaction for the RNA with suitable primers. Add ~15ul of the 
 

SECTION: RT-PCR
10. Spin-down the 1.5ml centrifuge tube (5 seconds)
TITLE: MC38 and B16-F10 culture media
AUTHORS: Elinor Gottschalk, Bulent Arman Aksoy, Pinar Aksoy, Jeff Hammerbacher

[STEPS]
?. [Mix components and sterile filter]
Working in the hood, remove 50 ml of DMEM from the 500 ml bottle (HyClone™ Dulbecco's High Glucose Modified Eagles Medium). To the remaining 450 ml DMEM add: 50 ml FBS (Fetal Plus) (10%) and 5 ml penicillin-streptomycin (1%).Sterile filter into a 500 ml 0.22 filter bottle. Store at  in .
4 °C
TITLE: Mass-Spectrometry analysis of ATP13A2 samples
AUTHORS: Sue  Sim, eunyong_park  

[DESCRIPTION]
Preparing mass-spectrometry samples to analyze polyamine content in purified ATP13A2 samples

[STEPS]
1. Pool purified ATP13A2 sample after SEC (sample should be in MS buffer after SEC)
2. Concentrate the protein to ~1.2 mg/mL using an Amicon Ultrafilter (cut-off 100kDa) at 4 °C

3. Prepare standards of spermidine (Sigma-Aldrich) and spermine (Sigma-Aldrich) at a concentration of 10 µM in MS buffer
4. Flash-freeze samples in liquid nitrogen and store at −80 °C until use
5. Dilute each sample 1:1 into acetonitrile with 1% formic acid (volume/volume)
6. Analyze by nanoelectrospray ionization (nanoESI) high-resolution mass spectrometry, using an LTQ-Orbitrap-XL mass spectrometer (Thermo Fisher Scientific)
6.1. Performed by QB3/Chemistry Mass Spectrometry Facility (University of California, Berkeley)
6.2. Mass spectrometer was equipped with a nanoESI source and operated in the positive ion mode
6.3. Mass spectra were acquired at a mass resolution setting of 100,000, as measured at mass-to-charge ratio (m/z) = 400, full width at half-maximum peak height
6.4. Mass spectrometry data acquisition and processing were performed using Xcalibur software (version 2.0.7, Thermo)
TITLE: Nano-DESI Mass Spectrometry Imaging kidney characterization pipeline for tissues collected by Vanderbilt University
AUTHORS: yang1832 , Julia Laskin

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Our goal is to map the biological tissues with Nano-DESI Mass Spectrometry Imaging and build an atlas of human kidney.</div><div class = "text-block">Scope: </div><div class = "text-block">Provide an overview of the sample preparation steps used by the Vanderbilt Tissue Mapping Center and Nano-DESI MSI workflow used by Julia Laskin's group as part of the Human Biomolecular Atlas Program (HuBMAP, NIH Common Fund).</div></div>

[STEPS]
?. Collection of post-surgical tissue by Vanderbilt.Collection:  dx.doi.org/10.17504/protocols.io.7gehjte
?. Stabilize and freeze tissues by VanderbiltFreezing Tissue:  dx.doi.org/10.17504/protocols.io.6wghfbw
?. Intial Rapid Pathology Assessment of Kidney Tissue by Vanderbilt.Staining: dx.doi.org/10.17504/protocols.io.4qngvveAssessment: dx.doi.org/10.17504/protocols.io.9dph25n
?. Cryosection tissues into micrometer thick sections, alternating between thaw mounting onto indium tin-oxide and positively charged glass slides (proceed to step 4), or collecting several tissue sections within an microcentrifuge tube for proteomics analysis by Vanderbilt.Cryosectioning:  dx.doi.org/10.17504/protocols.io.7ethjen
?. Perform autofluorescence microscopy on all tissue sections before IMS (step 5) by Vanderbilt.Autofluorescence:  dx.doi.org/10.17504/protocols.io.7e3hjgn
?. Perform high resolution IMS lipidomics and metabolomics analysis of tissue sections.IMS:  dx.doi.org/10.17504/protocols.io.bmwnk7de
?. Delipid the tissue section.Sample preparation: dx.doi.org/10.17504/protocols.io.bmw6k7he
?. Perform high resolution IMS proteomics analysis of tissue sections.IMS:  dx.doi.org/10.17504/protocols.io.bm2nk8de
?. Annotation of Lipids from IMS DataAnnotation: dx.doi.org/10.17504/protocols.io.bm2qk8dw
?. Annotation of proteins from IMS data.Annotation: dx.doi.org/10.17504/protocols.io.bm2rk8d6
?. Match the autofluorescence images from IMS sections with the ion images to visualize the correlation between molecules distribution and biological pathology.
?. Nano-DESI imaging mass spectrometry data processing.Data processing: dx.doi.org/10.17504/protocols.io.bm2sk8ee
TITLE: Protocol of sampling of feces in preterm infants
AUTHORS: Yuan Zhenya

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol refer to a study having the aim to study on the variation of gut microbiota in preterm infants in the process of feeding intolerance after birth.
In this study, fecal samples were collected from the preterm infants for several times and performed on the bacterial DNA extraction,PCR amplification in the lab, and 16S rRNA sequencing on the Miseq PE300 of the Illumina Platform.</div></div>

[STEPS]
?. [Fecal sampling]
Fecal samples were collected by disposable sterile swabs from the diapers of preterm infants, transferred to sterile tubes, and stored at -80℃ for the subsequent DNA extraction.
?. [DNA extraction]
The bacterial genomic DNA from samples was extracted by E.Z.N.A.® Soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.) according to the manufacturer’s protocols. The final DNA concentration and purification were determined by NanoDrop 2000 UV-vis spectrophotometer (Thermo Scientific, Wilmington, USA), and DNA quality was checked by 1% agarose gel electrophoresis.
?. [PCR]
The bacterial primer set of forward primer 338F  (5’-ACTCCTACGGGAGGCAGCAG-3’) and reverse primer 806R (5’-GGACTACHVGGGTWTCTAAT-3’) were used to amplify the DNA fragment of the 16S rRNA genes. TransStart Fastpfu DNA Polymerase and thermocycler PCR system (GeneAmp 9700, ABI, USA) were available for PCR. PCR reactions were performed in triplicate 20 μL mixture containing 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase and 10 ng of template DNA. Amplification conditions were as follows: An initial denaturation step of 95℃ for 3 min, followed by 27 cycles of the denaturation at 95℃ for 30 s, annealing at 55℃ for 30 s, elongating at 72℃ for 45 s with a final extension step at 72℃ for 5 min, halted at 10℃. The resulted PCR products were extracted from a 2% agarose gel and further purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantiFluor™-ST Real-time PCR System (Promega, USA) according to the manufacturer’s protocol before sequencing.
?. [16S rRNA sequencing]
16S rRNA genes sequencing was performed on an Illumina Miseq 300 Platform (Illumina, San Diego, USA) according to the standard protocols by Majorbio Bio-pharm Technology Co., Ltd., Shanghai, China. The raw reads were deposited into the NCBI Sequence Read Archive (SRA) database.
?. [Data analysis]
Raw fastq files were quality-filtered by Trimmomatic and merged by FLASH with the following criteria: (1)The reads were truncated at any site receiving an average quality score
TITLE: Multi-layer Hybrid Classification Model of COVID-19 Chest X-ray Images
AUTHORS: Thanakorn  Poomkur

[DESCRIPTION]
The coronavirus disease of 2019(COVID-19) has been declared a pandemic and has raised worldwide concern. Lung inflammation and respiratory failure are commonly observed in moderate-to-severe cases. Radiography or chest X-ray imaging is compulsory for diagnosis, and interpretation is commonly performed by skilled medical specialists. In this study, we propose anew computer-aided diagnosis (CADx) tool for identifying chest X-ray images of COVID-19 infection using a multi-layer hybrid classification model (MLHC). The MLHC-COVID-19 consists of two layers, Layer I: Healthy and non-Healthy; Layer II: COVID-19 and non-COVID-19. The MLHC-COVID-19 was evaluated in real COVID-19 cases. The classification results showed promising performance comparable with other existing techniques considering the accuracy, sensitivity, and specificity of 96.20%, 96.20%, and 0.971%, respectively. This demonstrates the effectiveness of the MLHC-COVID-19 in classifying chest X-ray images, enhancing the accuracy of chest X-ray image interpretation with a reduction in the interpretation time. Furthermore, a detailed comparison of the MLHC-COVID-19 with other techniques has been presented.

[STEPS]
2. 
 

1.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>For investigate the presence of YFV genome by quantitative PCR assays in the hydrolysis probe detection format. The target is the 5’-noncoding region (5’-NC) of the YFV genome (Domingo et al., 2012).</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Neuromuscular Junction Immunostaining 
AUTHORS: Jake Willows, Kristy Townsend, Magdalena Blaszkiewicz

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">A series of protocols to fluorescently label neuromuscular junctions in mice for qualitative and quantitative studies.</div></div>

[STEPS]
?. See attachments under Abstract for PDF copies of protocols.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Protocol for protein extraction, alkylation, and digestion to obtain LC/MS (liquid chromatography-mass spectrometry) data of calculated protein signal intensities in HEK-293 cells to monitor them.</p>

[BEFORE_START]
<p>Prepare a Krebs-Ringer-Buffer: </p>
<ul>
<li>154 mM NaCl</li>
<li>5.6 mM KCl</li>
<li>5.5 mM glucose</li>
<li>20.1 mM HEPES pH 7.4</li>
<li>25 mM NaHCO<sub>3</sub></li>
</ul>
<p> </p>
<p> </p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p><span style="font-weight: 400;">Electroporation of V. natriegens cells is a rapid 2-hour protocol, from culture inoculation to plating. Each transformation produces &gt;10^5 CFU / µg plasmid DNA. Each transformation requires 10mL of LB3 (high-salt) media, corresponding to 50uL of 200x concentrated cells.</span></p>

[BEFORE_START]
<ul>
<li>Prepare 1M ice cold sorbitol</li>
<li>Prepare "LB3" high-salt media as described below. To determine the volume of media you'll need, multiple the number of samples by 10mL. For example, if you want to transform 6 different samples, you need 60mL.</li>
</ul>
<ol>
<li><span style="text-decoration: underline;">Short LB3 protocol:</span> dissolve 25g LB Broth Miller (Fisher BP9723-500, ensure LB contains 10g of NaCl) and 20g NaCl in 1L total of deionized water, pH ~7.2. (alternatively, use the full LB3 protocl below). </li>
<li><span style="text-decoration: underline;">Full LB3 protocol:</span> dissolve 10g tryptone, 5g peptone, 30g NaCl in 1L total of deionized water. pH ~7.2.  </li>
</ol>
<ul>
<li>To make LB3 plates, use the protocl above and add agar to 1.5% final. </li>
</ul>

[STEPS]
?. 
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?.
TITLE: Zeiss AxioImager Multi Channel 20X Image Capture
AUTHORS: Allen Institute for Brain Science

[DESCRIPTION]
This protocol describes the capture of 20X multi-channel images using human and mouse tissue.

Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

[STEPS]