TITLE: Buffer Recipes for Protein Purification
AUTHORS: Annie Kwon

[DESCRIPTION]
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[STEPS]
?. [Equilibration buffer]
25 mM HEPES, 100 mM NaCl, pH 7.5  ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)1.461 g2.922 g5.844 g11.688 g23.376 g25 mM HEPES, 500 mM NaCl, pH 7.5  ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)7.305 g14.61 g29.22 g58.44 g116.88 g
ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)1.461 g2.922 g5.844 g11.688 g23.376 g
ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)7.305 g14.61 g29.22 g58.44 g116.88 g
?. [Lysis buffer]
50 mM Na/K phosphate buffer, 500 mM NaCl, 2.5% glycerol, pH 8.0  ABCDEF1250 mL500 mL1 L2 L4 L2NaH2PO4 • H2O (MW=137.9923)0.0690 g0.1380 g0.2760 g0.5520 g1.1039 g3K2HPO4 (MW=174.18)2.0902 g4.1803 g8.3607 g16.7213 g33.4426 g4NaCl (MW=58.44)7.305 g14.61 g29.22 g58.44 g116.88 g5glycerol6.25 mL12.5 mL25 mL50 mL100 mL
ABCDEF1250 mL500 mL1 L2 L4 L2NaH2PO4 • H2O (MW=137.9923)0.0690 g0.1380 g0.2760 g0.5520 g1.1039 g3K2HPO4 (MW=174.18)2.0902 g4.1803 g8.3607 g16.7213 g33.4426 g4NaCl (MW=58.44)7.305 g14.61 g29.22 g58.44 g116.88 g5glycerol6.25 mL12.5 mL25 mL50 mL100 mL
?. [Wash buffer]
25 mM HEPES, 1M NaCl, 10 mM imidazole, 10% glycerol, pH 7.5  ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)14.61 g29.22 g58.44 g116.88 g233.76 g4imidazole (MW=68.08)0.1702 g0.3404 g0.6808 g1.3616 g2.7232 g5glycerol25 mL50 mL100 mL200 mL400 mL25 mM HEPES, 1M NaCl, 10 mM imidazole, 10% glycerol, pH 7.5   ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)14.61 g29.22 g58.44 g116.88 g233.76 g4imidazole (MW=68.08)0.3404 g0.6808 g1.3616 g2.7232 g5.4464 g5glycerol25 mL50 mL100 mL200 mL400 mL
ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)14.61 g29.22 g58.44 g116.88 g233.76 g4imidazole (MW=68.08)0.1702 g0.3404 g0.6808 g1.3616 g2.7232 g5glycerol25 mL50 mL100 mL200 mL400 mL
ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)14.61 g29.22 g58.44 g116.88 g233.76 g4imidazole (MW=68.08)0.3404 g0.6808 g1.3616 g2.7232 g5.4464 g5glycerol25 mL50 mL100 mL200 mL400 mL
?. [Elution buffer]
25 mM HEPES, 100 mM NaCl, 300 mM imidazole, pH 7.5  ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)1.461 g2.922 g5.844 g11.688 g23.376 g4imidazole (MW=68.08)5.106 g10.212 g20.424 g40.848 g81.696 g25 mM HEPES, 500 mM NaCl, 300 mM imidazole, pH 7.5  ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)7.305 g14.61 g29.22 g58.44 g116.88 g4imidazole (MW=68.08)5.106 g10.212 g20.424 g40.848 g81.696 g
ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)1.461 g2.922 g5.844 g11.688 g23.376 g4imidazole (MW=68.08)5.106 g10.212 g20.424 g40.848 g81.696 g
ABCDEF1250 mL500 mL1 L2 L4 L2HEPES (MW=238.31)1.4894 g2.9789 g5.9578 g11.9155 g23.831 g3NaCl (MW=58.44)7.305 g14.61 g29.22 g58.44 g116.88 g4imidazole (MW=68.08)5.106 g10.212 g20.424 g40.848 g81.696 g