TITLE: List of IHC/WB antibodies, RT-PCR probes and software used
AUTHORS: carmela.giachino

[DESCRIPTION]
This protocol details list of antibodies used for immunohistochemistry and western blot.

[STEPS]
SECTION: List of antibodies used for immunohistochemistry and western blot
1. Table 1. List of antibodies used for immunohistochemistry and western blot.

 
        Antibody   name  Company  Dilution  RRID    CCR2, goat   polyclonal  Thermo Fisher Scientific  0.388889  AB_557978    CD11b, rabbit  Abcam  0.736111  AB_2650514    CD11b, rat  Biolegend  0.388889  AB_312784    CD3, mouse  Santa Cruz Biotechnology  0.388889  AB_2228831    CD4, rat  Bio-Rad  0.25  AB_1898234    CD68, rat  Bio-Rad  0.25  AB_2074849    DAT, rabbit  Proteintech  0.180556  AB_2879116    GFAP, mouse  Sigma  0.215278  AB_2827276    GFAP, rabbit  sigma  0.736111  AB_2905668    GSK-3β, mouse  ECM Biosciences  0.180556  AB_2115216    HuD+HuC, rabbit  Abcam  0.319444  AB_2864321    Iba-1, goat   polyclonal  Abcam  0.180556  AB_870576    Iba-1, rabbit   polyclonal  Wako  0.388889  AB_2889406    iNOS, mouse  Cell signaling technology  0.180556  AB_1078202    LRRK2, rabbit  clone 41-2, Abcam by MJFF  0.736111  AB_2713963    MAP2, rabbit  Cell Signaling  0.388889  AB_10693782    NeuN, rabbit  Abcam  0.736111  AB_2716282    Nurr1, goat   polyclonal  R&D  0.25  AB_2153894    phospho-alpha-Synuclein   (Ser129), rabbit monoclonal (clone LS4-1B1)  Millipore  0.736111  AB_673008    phospho-GSK-3β   (p-Tyr216), rabbit  Abcam  0.388889  AB_2533691    Phospho-LRRK2-S935,   rabbit  Abcam by MJFF  0.736111  AB_2864018    phospho-Tau   (pSer396), rabbit polyclonal  Sigma aldrich  0.180556  AB_261757    phospho-Tau   (pThr181)  Thermo Fisher scientific  0.180556  AB_1087704    Tau, mouse  RayBiotech  0.180556  AB_11217610    TH, mouse  STEMCELL Technologies  0.180556  AB_215512    TH, rabbit  Pel-Freez  0.180556  AB_2313713    TH, sheep  Pel-Freez Biologicals  0.180556  AB_2935637    TH, sheep  Pel-Freez Biologicals  0.736111  AB_461070    TUBB3, mouse  Biolegend  0.319444  AB_2313773    α-syn (C-20)-R,   rabbit  Santa Cruz Biotechnology  0.180556  AB_2192953    α-syn aggregate,   MJFR-14-6-4-2, rabbit  Abcam  Jan-00  AB_2714215    β-actin, rabbit  Cell Signaling  0.736111  AB_10694076  
 
SECTION: List of antibodies used for immunohistochemistry and western blot
2.  

 Table 2. List of probes and IDs used for quantitative Real time PCR.
 
 
      PROBES  IDs    Casp6  Mm00438053-m1    CCl3  Mm 00441258-m1    CxCl10  Mm 00445235-m1    CxCl11  Mm00444662-m1    Drd1a  Mm01353211-m1    Drd2  Mm00438545-m1    Foxa2  Mm01976556-s1    GAPDH  Mm99999915-g1    Gfap  Mm 00546086-m1    Gp91phox  Mm01287743-m1    GSK3b  Mm00444911-m1    IL-10  Mm12088386-m1    IL-17  Mm00439618-m1    IL-18  Mm00434226-m1    Il1b  Mm00434228-m1    IL-4  Mm00445259-m1    Itgam (Mac1)  Mm 00434455-m1    LRRK2  Mm00481934_m1    Map2  Mm00485231-m1    Nfkb1  Mm00476361-m1    Nos 2  Mm 00440485-m1    Nurr 1  Mm 00443056-m1    Slc6a3 (Dat)  Mm 00438388-m1    Tau (Mapt)  Mm00521988-m1    Th  Mm 00447546-m1    Tnf  Mm00443258-m1    Tnfrsf1b  Mm00441875-m1    α-syn (SNCA)  Mm00458965-m1  
 
 

SECTION: List of antibodies used for immunohistochemistry and western blot
3.  Table 3 List of software 
     Software name      Version      URL        ImageJ      1.53t      https://imagej.net/        Luminex xPONENT      4.3         https://www.luminexcorp.com/xponent/        Bio-Plex Manager      6.2         http://www.bio-rad.com/en-us/product/bio-plex-manager-software-standard-edition           ImageQuantity One      4.6      http://www.bio-rad.com/en-us/product/quantity-one-1-d-analysis-software        GraphPad Prism      9      http://www.graphpad.com/