eamag/protocols-with-steps huggingface dataset:
TITLE: SEM General Fixation
AUTHORS: Elizabeth Fozo

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">General SEM Fixation Procedure</div></div>

[STEPS]
?. [General SEM Fixation Procedure]
Grow 50mL cells to appropriate OD600 and wash in 1X PBS. Pour of supernatant.
?. [General SEM Fixation Procedure]
Primary Fixation:3% Gultaraldehyde in 0.1M cacodylate (1X PBS)* = add 500uL and vortex brieflyFix for 1 hour at room temperature.*Any buffer should be fine = use 1X PBS
?. [General SEM Fixation Procedure]
Rinse the sample in a buffer (1X PBS) at least 3 times for 10 minutes each.
?. [General SEM Fixation Procedure]
Secondary Fixation: (Not usually required)2% Osmium tetroxide in 0.1M cacodylate (1X PBS) = add 200uLFix for 1 hour at room temperature.
?. [General SEM Fixation Procedure]
Rinse the sample in the water at least 3 times for 10 minutes each.****If working with single cells such as bacteria, allow the sample to settle onto a 3X4mm silicon chip after the final water wash. The Si chip (clean off with Methanol – cells go on SHINY side) is then processed through the remaining dehydration steps
?. [General SEM Fixation Procedure]
Put the samples at 4oC overnight in 500uL 1X PBS
?. [General SEM Fixation Procedure]
Spin down before heading to JIAM. – Don’t resuspend
?. [General SEM Fixation Procedure]
Bring samples to JIAM – SEM facility
?. [General SEM Fixation Procedure]
Clean the silicon chip with methanol
?. [General SEM Fixation Procedure]
Poly-L-lysine – add 20uL to the chip and let sit for 2 minutes – blow the liquid off with compressed air
?. [General SEM Fixation Procedure]
Add 20uL of cells to the silicone chip and let sit for 25 minutes.
?. [General SEM Fixation Procedure]
Dehydrate sample through a graded ethanol series25% Ethanol                10 min.50% Ethanol                10 min.70% Ethanol                10 min.95% Ethanol                10 min.100% Ethanol              10 min100% Dry Ethanol       10 min – Freshly opened container of anhydrous ethanolThe purpose of 2-100% Ethanol steps is to account for any water that transfers from 95%.
?. [General SEM Fixation Procedure]
Critical point dry – Follow the protocol
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>In this protocol we describe some approaches to graphically represent single profiled samples or a merged table of relative abundances.</p>

[BEFORE_START]
REQUIREMENTS: <a href="https://bitbucket.org/nsegata/graphlan" target="_blank">GraPhlAn</a> installed (and in the system path), and the <a href="http://matplotlib.org/" target="_blank">matplotlib</a> python library. GraPhlAn can be downloaded using <a href="http://mercurial.selenic.com/" target="_blank">Mercurial</a>: <br /><code>hg clone ssh://hg@bitbucket.org/nsegata/graphlan</code>.

[GUIDELINES]
The annotation file can be edited manually and/or with ad-hoc scripts for a higher degree of customization. Please refer to the <a href="https://bitbucket.org/nsegata/graphlan" target="_blank">GraPhlAn readme file</a> and to these <a href="https://bitbucket.org/nsegata/graphlan/wiki/Home" target="_blank">examples</a> for additional information.<br /><br />The same script can also be applied to the relative abundance table of 20 merged samples. In this case, the abundances reported refer to the overall average of each clade across all the samples in the table.<br /><br />
<pre>$ plotting_scripts/metaphlan2graphlan.py output/merged_abundance_table.txt  --tree_file tmp/merged.tree.txt --annot_file tmp/merged.annot.txt
$ graphlan_annotate.py --annot tmp/merged.annot.txt tmp/merged.tree.txt tmp/merged.xml
$ graphlan.py --dpi 200 tmp/merged.xml output_images/merged.png</pre>
<p>As an example of manually and script-based modification of the "annotation" file provided in <a href="https://bitbucket.org/nsegata/metaphlan/wiki/annotation_example.txt" target="_blank">this file</a>, we can produce comparisons between classes. Note, this script also includes information about biomarkers (detailed in Step 4: Taxonomic Biomarker discovery with LEfSe). The resulting GraPhlAn image is reported below:<br /><br /><br /><img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/bvyc2w6.png" alt="" width="500" data-src="https://s3.amazonaws.com/pr-journal/bvxc2w6.png" data-ofn="TD_vs_BM_tree.png" /><br /><br /><br />Notice also that MetaPhlAn output can be exported to <a href="http://sourceforge.net/p/krona/home/krona/" target="_blank">Krona</a> (another popular visualization tool) using the script <code>metaphlan2krona.py</code> in the<code>conversion_scripts</code> folder downloaded as part of the MetaPhlAn package.</p>
The commands reported in this protocol can be retrieved as a <a href="https://bitbucket.org/nsegata/metaphlan/wiki/3_graphlan_visualizations.sh" target="_blank">bash script</a>.

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.</p>
<p> </p>
<p> </p>
<p>Modified from: Watanabe, Makoto M. "Fresh-and salt-water forms of Spirulina platensis in axenic cultures." <em>Bull. Jpn. Soc. Phycol.</em> 25 (1977): 371-377.</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Analysis of Factors Affecting Gelatin Degradation Assay and Countermeasures
AUTHORS: 181830691 

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left">Edible gelatin belongs to the animal gelatin category of hydrophilic colloid/edible gelatin, which is made from the collagen part of connective tissues such as animal skin, bone, muscle membrane and muscle enchantment. Gelatin has many excellent properties, which makes it a good hand in food processing industry.</div></div><div class = "text-block"><div class = "justify" style = "text-align:left"></div></div><div class = "text-block"><div class = "justify" style = "text-align:left"><span>The main factors affecting the </span><a href="https://www.creative-proteomics.com/services/gelatin-degradation-assay.htm" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;:UNDERLINE;">gelatin degradation assay</span></a><span> are temperature, bacteria and pH.</span></div></div></div>

[STEPS]
TITLE: Risk factors associated with IgA vasculitis with nephritis (Henoch–Schönlein purpura nephritis) progressing to unfavorable outcomes: a meta-analysis
AUTHORS: Qiu Li

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The aim of this study was to analyze  the risk factors associated with unfavorable outcomes in children with IgA vasculitis with nephritis (Henoch-Schőnlein purpura nephritis) (IgA-VN). these databases PubMed, Embase, and Web of Science were searched in this meta-analysis. The data were extracted to perform pooled analysis, heterogeneity testing, subgroup analysis, sensitivity analysis, and publication bias analysis.</div></div>

[STEPS]
?. literature search: PubMed, Embase, and Web of Science databases were searched for studies, published in English through February 2019. Using basic search terms from combined text and Medica Subject Heading (MeSH) terms. These included a MeSH search using the term ‘Purpura, Schoenlein-Henoch’ and a keyword search using the term ‘Henoch-Schönlein purpura’, and terms related to unfavorable outcomes (including MeSH searches using the terms ‘Kidney Failure, Chronic’ and ‘Renal Insufficiency, Chronic’, and keyword searches using the term ‘end stage renal disease’ and ‘chronic renal disease’).
?. Study selection: Cohort and case-control studies were included, whereas cross-sectional, case reports, review articles, comments, meeting abstracts, genetic association studies, and editorial comments were excluded. Studies were included if they assessed patients diagnosed with IgA-VN at age
?. Data collection and data extraction: Data were independently extracted by two investigators, with any discrepancies resolved by a third investigator. Data collected included the characteristics of the studies (year of publication, country, and duration of follow-up), the demographic characteristics of the patients (e.g., numbers of patients and age), laboratory predictors, renal manifestations and renal histopathology at onset.
?. Quality assessments of the studies: Study quality was assessed using three main categories of the Newcastle-Ottawa scale.
?. Statistical analysis: Aggregates were pooled used the generic inverse variance meta-analysis method. Effect measures of interest were reported as ORs and 95% CIs.All statistical analyses were performed using Stata 14.0 software.
TITLE: Tissue Culture (tc) PBS
AUTHORS: Jacquelina.Woods  

[DESCRIPTION]
This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV-2 variants in wastewater​​. Protocols developed for this project cover wastewater collection, concentration, RNA extraction, RT-qPCR detection, library prep, genome sequencing, quality control checks, and data submission to NCBI. This method provides a reagent formula required in the rapid concentration of intact viruses from wastewater using a combination of PEG precipitation and ultracentrifugation. 

[STEPS]
SECTION: 1X PBS
1. Dissolve components in deionized or ultrapure water to 1L. 
SECTION: 1X PBS
1.1. 8.0 g  

SECTION: 1X PBS
1.2. 0.2 g  

SECTION: 1X PBS
2. Adjust pH 7.5

SECTION: 1X PBS
1.3. 0.12 g  

SECTION: 1X PBS
1.4. 0.91 g 

SECTION: 1X PBS
3. Autoclave 121 °C15 min

SECTION: 1X PBS
4. Store at 2-8 °C

 

SECTION: 10X tc PBS
5.
TITLE: TFome CRISPRko screens
AUTHORS: Andrea R Daniel

[DESCRIPTION]
This protocols describes methods for transcription factor CRISPR knockout screens to reveal cofactors of BATF3 in T cells. 

[STEPS]
SECTION: gRNA library construction
1. The Brunello genome-wide KO83 library (four gRNAs per gene) was subset for 1,612 TFs45 and IL7R. 
SECTION: gRNA library construction
2. A total of 550 NT gRNAs were included in the library for a total of 7,000 gRNAs (available in Supplementary Table 6 of McCutcheon et al. Nature Genetics, 2023. https://doi.org/10.1038/s41588-023-01554-0). 
SECTION: gRNA library construction
3. This gRNA library was cloned into SpCas9 gRNA lentiviral plasmids with either mCherry or BATF3.
SECTION: gRNA library cloning
4. Oligonucleotide pools containing variable gRNA sequences and constant regions for polymerase chain reaction (PCR) amplification were synthesized by Twist Bioscience.
SECTION: gRNA library cloning
5. gRNA amplicons were gel extracted, PCR purified and input into 20 μl Gibson reactions (5:1 molar ratio of insert to backbone) with 200 ng of Esp3I digested and 1 × solid-phase reversible immobilization (SPRI)-selected (Beckman Coulter) plasmid backbone. 
SECTION: gRNA library cloning
6. Gibson reactions were purified using ethanol precipitation and transformed into Lucigen’s Endura ElectroCompetent Cells. 
SECTION: gRNA library cloning
7. Transformed cells were cultured overnight and plasmids were isolated using Qiagen Midi Kits.
SECTION: TFome CRISPRko screens
8. A total of 20 × 106 CD8+ T cells from two donors were activated with CD3/CD28 dynabeads at a 1:1 ratio.
SECTION: TFome CRISPRko screens
9. At 24 h post-activation, CD8+ T cells were split evenly and transduced in parallel with TFome CRISPRko gRNA libraries with mCherry or BATF3.
SECTION: TFome CRISPRko screens
10. At 48 h post-activation, cells were electroporated with Cas9 protein. Briefly, the cells were collected, spun down at 90g for 10 min, resuspended in 100 μl of Lonza P3 Primary Cell buffer with 3.2 μg Cas9 (Thermo) per 106 cells, and electroporated with the pulse code EH115. 
SECTION: TFome CRISPRko screens
11. After electroporation, warm medium was immediately added to each cuvette and cells were recovered at 37 °C for 20 min before being transferred into a six-well plate. 
SECTION: TFome CRISPRko screens
12. On day 3 post transduction, cells were selected with 2 μg ml−1 of puromycin for 3 days. On day 9 post transduction, cells were stained for CD8, IL7R and a viability dye. 
SECTION: TFome CRISPRko screens
15. Viable CD8+ T cells in the lower and upper 10% tails of IL7R expression were sorted for subsequent gRNA library construction and sequencing. 
SECTION: TFome CRISPRko screens
12.1.  
 
 
SECTION: TFome CRISPRko screens
13. An SH800 FACS Cell Sorter (Sony Biotechnology) was used for cell sorting and analysis.
SECTION: TFome CRISPRko screens
14. Cells were then washed with flow buffer, spun down at 300g for 5 min and resuspended in flow buffer for cell sorting.
SECTION: TFome CRISPRko screens
16. All replicates were maintained and sorted at a minimum of 75× coverage.
TITLE: ViriCan Protocol: PBMC isolation from whole blood
AUTHORS: Deilson  Elgui De Oliveira

[DESCRIPTION]
Protocol for isolation of peripheral blood mononuclear cells for downstream applications requiring viable cells or cell pellets to extract DNA or RNA.

[GUIDELINES]
Notes:

From Thermofisher (Answer Id: E4470): "Blood collected with EDTA typically has the highest DNA contamination, blood collected with heparin typically has less than that collected with EDTA, and blood collected with citrate shows the least DNA contamination of the three. (Formulation for citrate solution: 3.8% (w/v) which is 3.8 g/100 mL of water. Use 0.5 mL for every 4.5 mL of blood. Rock gently back and forth after adding citrate solution to mix.) Adding 12 µL of 5 N acetic acid per milliliter of TRIzol Reagent may help, although there may still be a problem with DNA contamination. Using plasma or serum works best. The fresher the blood sample the better the RNA. Degraded RNA has been observed in blood that has been processed in as little as two hours after drawing."

Reagent manuals:
Ficoll-Paque Plus: https://drive.google.com/file/d/1hlvoGTZ60NTTsKaTwFiznoF1eh4vufwm/
Histopaque 1077: https://drive.google.com/file/d/1jgZXuhhGaw0cCRLKa9K_1W2KaOLgPhHq/

[STEPS]
SECTION: Blood colection
1. Collect 4 mL of whole blood in tubes with anticoagulant*


 


SECTION: PBMC isolation
4. Centrifuge  Room temperature at 400 x g, 30 min, 
  
 


SECTION: PBMC isolation
2. Add 3 mL of Histopaque/Ficoll to 15mL conical centrifuge tube

 


SECTION: PBMC isolation
3. Carefully layer about 3 mL of whole blood on top of the Histopaque/Ficoll reagent.

 

SECTION: PBMC isolation
5. Carefully transfer the middle opaque phase containing the PBMC to a new identified tube as follows and proceed to obtain PBMC's lisates or viable PBMCs for cellular assays.
 

SECTION: (Optional) PBMC clearing
8. To the 15mL conical centrifuge tubes, add 10 mL of on ice isotonic   

SECTION: (Optional) PBMC clearing
8.1. Centrifuge 250 x g, 10 min   

SECTION: (Optional) PBMC clearing
8.2. Aspirate the supernatant and discard
8.4. Resuspend the cell pellet with 5.0 mL isotonic  
8.5. Mix by gentle aspiration with a Pasteur pipet or pipetting up and down with 1mL tip
8.6. Centrifuge 250 x g, 10 min   
8.7. Discard supernatant
8.8. Repeat the PBMC wash procedure twice
9. Proceed to downstream applications with the isolated PBMCs
SECTION: (Optional) PBMC clearing
8.3. Wash the PBMC pellet
SECTION: (Optional) PBMC lysis to extract DNA/RNA
6. Pellet the cells by centrifugation 1000 x g, 5 min, 4 °C 
 

SECTION: (Optional) PBMC lysis to extract DNA/RNA
6.1. Add To the PBMCs pellet:

 250 µL of TRIZol LS Reagent

(General recommendation: 1 mL of TRIZol per 5-10 ×10e6 eukaryotic cells)



SECTION: (Optional) PBMC lysis to extract DNA/RNA
6.3. Store PBMC lisates in Trizol at -70 °C or immediately proceed to nucleic acid isolation 
 


SECTION: (Optional) PBMC lysis to extract DNA/RNA
7. 
SECTION: (Optional) PBMC lysis to extract DNA/RNA
6.2. Homogenize to lysate the cells
TITLE: DNA Maxwell RSC instrument
AUTHORS: Yin-Tse Huang,  Tina

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Maxwell automated nucleotide extraction </div></div>

[STEPS]
?. 先取 或 ，將sample放入研缽加液態氮磨
[plate]
[1.5 ml tube]
?. 加note: 10ul Lyticase = 100U
[Lyticase]
37 °C
?. 加入 等待溶解，並移到離心管
[CTAB]
?. +
94 °C
[Heating]
[Cooling]
?. + into離心管
[RNase A]
[Proteinase K]
?. 每10～15分鐘搖晃一次
[加熱]
?. Vortex tube 使均勻混合
?. Centrifuge: 16000 34, 10 min, 24 10
?. 加 + 到第一格
[Lysis buffer]
[上清液Sample]
?. 加 到第八格的小離心管 蓋子不要關
[Elution buffer]
?. 放入機器讓他跑
?. 把磁棒放到長條的最後一格
TITLE: Assembly_Template
AUTHORS: ann.mccartney  

[DESCRIPTION]
ERGA_Assembly_Strategy

[STEPS]
SECTION: Assembly and Curation
1. 
Data Download

 

SECTION: Assembly and Curation
2. Data cleaning
 

SECTION: Assembly and Curation
3. Assembly of long reads
 
2880 min 
 

 


SECTION: Assembly and Curation
4. Scaffolding with HiC
 

 





SECTION: Assembly and Curation
5. Polishing with Illumina
 

 

 

SECTION: Assembly and Curation
6. QC
 

 

 

SECTION: Assembly and Curation
7. Curation
TITLE:     ELISA for quantification of IL-16 in human serum.
AUTHORS: Angel  Justiz-Vaillant

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. </div><div class = "text-block"><span>Eosinophils and CD8+T cells synthesize IL-16. Its principal target is CD4+ T cells. It causes CD4+ T cell chemoattraction. </span><a href="https://www.ncbi.nlm.nih.gov/books/NBK499840" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;">[1]</span></a></div><div class = "text-block">Reference</div><div class = "text-block"><span>1. Justiz Vaillant AA, Qurie A. Interleukin. In:</span><span style = "font-style:italic;">StatPearls</span><span>. Treasure Island (FL): StatPearls Publishing; June 12, 2019.</span></div></div>

[STEPS]
?. An anti-human IL-16 coating antibody is adsorbed onto microwells by incubation overnight at 4°C.
?. Add 50 µl of human serum. Human IL-16 present in the serum sample binds to antibodies adsorbed to the microwells.
?. The microplate is blocked with 3% non-fat milk-PBS buffer and later wash to remove unbound proteins.
?. Fifty (50) µl of biotin-conjugated anti-human IL-16 antibody is added. The optimal dilution must be investigated.
?. The microplate is rewashed with PBS-Tween 20 buffer, pH 7.4.
?. One hundred µl of streptavidin-HRP conjugate is added and it binds to the biotin-conjugated anti-human IL-16 antibody. The optimal dilution of this conjugate must be investigated.
?. The plate is washed following incubation to remove the unbound Streptavidin-HRP.
?. Add 100 µl of 3',3',5',5'- tetramethylbenzidine (TMB; Sigma-Aldrich) to each well.
?. Incubate the microwells in the dark for 15 min.
?. A colored product is formed in proportion to the quantity of human IL-16 present in the sample or standard.
?. The reaction is terminated by addition of 100 µl 3M H2SO4  and absorbance is measured at 450 nm.
?. A standard curve is made from 7 human IL-16 standard dilutions and the human IL-16 sample concentration is determined.
?. For better results place the microplate on a plate shaker in every incubation.
TITLE: Preparation and enzyme activity of recombinant protein
AUTHORS: Ning Zhang

[STEPS]
?. ligate CTSL sequence with pET28a vector.
?. Grow small culture overnight.
?. Inoculate 1ml overnight culture into 100ml 2xYT media in a 500 ml flask.
?. Incubate in a 37°C shaker until OD600 reaches 0.6 (About 3.5 hours), and then add 100 mM IPTG 100ul and incubate for 3 hr.
?. Pellet the bacteria at 6, 000 rpm for 5-6min, resuspend in 9ml PBS containing protease inhibitors and transfer to 50 ml conical tubes.
?. Sonicate the bacterial suspension on ice, 4 times x 1min with 30s rests (powder setting: cup, 10; tip, 5)
?. Transfer the resulting sonicate to a 10-15 ml conical tube and incubate with 1ml 10% Triton X-100 for 30min at 4°C on a rocker
?. Transfer the lysates to high-speed tubes (usually 12-14ml falcon tubes from BD or VWR), and centrifuge at 11, 000rpm at 4°C to remove unlysed bacteria and cell debris from sonicate. Keep the supernatant, and repeat the step 7 once
?. Keep the supernatant in 15ml conical tubes
?. Add His beads (200-250ul) and incubate for 1h at RT with constant rocking.
?. Pellet the beads at 2, 000 rpm for 2 min, 4°C, and aspirate the supernatant.
?. Wash the beads 3 times with PBS, 5 min each at RT with rocking
?. Resuspend in 300ul PBS
?. Store the His beads at 4°C
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
Objective: To evaluate the expression of miR-155, nuclear factor kappa B (NF-κB) and soluble intercellular adhesion molecule-1 (sICAM-1) in the peripheral blood of patients with type 2 diabetes mellitus (T2DM) and assess its relationship with diabetic vascular lesions.
 
Methods: A total of 165 T2DM patients and 60 healthy patients were included. Patients were divided into two groups: a simple T2DM group and a T2DM with vascular lesion group. The latter was further divided into a microvascular lesion group, a macrovascular lesion group and a microvascular combined with macrovascular lesion group. Albuminuria levels were divided into three groups as follows: <30 mg / 24 h, normal albuminuria group (NAU group); 30-300 mg / 24 h, microalbuminuria group (MAU group); and ≥300 mg / 24 h, large albuminuria group (LAU group). Levels of peripheral blood miR-155, NF-κB, and sICAM-1 were detected by RT-PCR[SE .
Results:
①Compared with that in the control group, the expression of miR-155, NF-κB, and sICAM-1 in the T2DM group was significantly increased [(1.00±0.12) vs. (1.82±0.71), (1.04±0.33) vs. (2.28±0.66), (1.03±0.30) vs. (1.88±0.80), respectively; P<0.05].
②The levels of miR-155, NF-κB, and sICAM-1 were significantly higher in the T2DM vascular lesion group than in the simple T2DM group [(1.95±0.73) vs. (1.34±0.29), (2.40±0.65) vs. (1.65±0.16), (2.01±0.77) vs. (1.07±0.41), respectively; P<0.05].
③The expression of miR-155 in the microvascular combined with macrovascular lesion group was higher than that in the macrovascular lesion group [(2.36±0.61) vs. (1.77±0.59); P<0.05], and the level of NF-κB in the former group was significantly higher than those in the microvascular and macrovascular lesion groups (P<0.05).
④Compared with that in the NAU group, the level of miR-155 was significantly increased in the MAU and LAU groups [(1.04±0.20) vs. (1.41±0.49) vs. (2.64±0.52), respectively; p<0.05], and the miR-155 level in the LAU group was higher than that in the MAU group (P<0.05).
⑤Pearson correlation analysis showed that miR-155 was positively correlated with NF-κB and sICAM-1 (R=0.259, 0.67; P<0.01).
Conclusions: miR-155 expression was increased in T2DM patients and vascular lesions, consistent with the increasing trends observed for NF-κB and sICAM-1. These findings suggest that miR-155 may be involved in the occurrence and development of diabetic chronic vascular disease.
     
 

[STEPS]
SECTION: Sujects
?. 
?. 
SECTION: Incriteria
?. 
SECTION: Exclusion criteria
?. 
SECTION: Grouped
?. 
SECTION: Methods
?. 
SECTION: Methods
?.