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[GUIDELINES]
Materials:<br /><strong>Part 1:</strong><br />Black 96-well microtiter plate<br />Pipet<br />25% glutaraldehyde<br />Liquid nitrogen<br /><br /><strong>Part II:</strong><br />None<br /><br /><strong>Part III:</strong><br />10<sup>8</sup> cell hosts &amp; 10<sup>7</sup> phages for one-step<br />~240 ml host for plating<br />70 ml SN media<br />950 ml of SN top agar<br />480 ml of SN bottom agar<br />Water bath<br />Vortex<br />Micropipettes and tips<br />Electric pipette<br />66 --&gt; 1 ml serological pipettes<br />1 --&gt; 1L tissue culture flask<br />231 --&gt; 15 ml snap cap tubes<br />230 --&gt; SN bottom agar plates (60x15mm)<br />33 --&gt; 1 ml luer-lock syringes<br />33 --&gt; 0.2 µm syringe filters (25mm diameter)<br />10 --&gt; 50 ml conical tubes<br />290 --&gt; 1.5 µl centrifuge tubes<br /><br /><br /><span style="text-decoration: underline;"><strong>Phage Growth Parameters:</strong></span><br /><br /><br />
<table>
<tbody>
<tr>
<td>Phage(s)</td>
<td>Morphology</td>
<td>Burst Size</td>
<td>Latent Period</td>
<td>Host</td>
<td>Citation</td>
</tr>
<tr>
<td>Syn5</td>
<td>podovirus</td>
<td>20-30</td>
<td>1 hr</td>
<td>WH8109</td>
<td>Raytcheva et al. (2010)</td>
</tr>
<tr>
<td>S-CBP1, S-CBP2, S-CBP3</td>
<td>podovirus</td>
<td>75-92</td>
<td> </td>
<td> </td>
<td>Wang (2007)</td>
</tr>
<tr>
<td>P60</td>
<td>podovirus</td>
<td>81</td>
<td>1 hr</td>
<td>WH7803</td>
<td>Brown et al (2006)</td>
</tr>
<tr>
<td>P-SSP7</td>
<td>podovirus</td>
<td> </td>
<td>8 hrs</td>
<td>Med4</td>
<td>Lindell et al (2007)</td>
</tr>
<tr>
<td>S-PM2*</td>
<td>myovirus</td>
<td>21</td>
<td>9 hrs</td>
<td>WH7803</td>
<td>Wilson et al (1996)</td>
</tr>
<tr>
<td>S-CAM4</td>
<td>myovirus</td>
<td>~50</td>
<td> </td>
<td>WH7803</td>
<td>Kuznetsov et al (2010)</td>
</tr>
<tr>
<td>S-TIM5</td>
<td>myovirus</td>
<td>~35</td>
<td>6 hrs</td>
<td>WH8102</td>
<td>Sabehi et al (2012)</td>
</tr>
<tr>
<td>S-CBM2</td>
<td>myovirus</td>
<td>28</td>
<td> </td>
<td>CB0101</td>
<td>Wang (2007)</td>
</tr>
<tr>
<td>S-RIM1</td>
<td>myovirus</td>
<td>63</td>
<td>6 hrs</td>
<td>WH7803</td>
<td>Stoddard et al (2007)</td>
</tr>
<tr>
<td>S-RIM8</td>
<td>myovirus</td>
<td>86</td>
<td>8 hrs</td>
<td>WH7803</td>
<td>Stoddard et al (2007)</td>
</tr>
<tr>
<td>S-CBS2, S-CBS3, S-CBS3</td>
<td>siphovirus</td>
<td>57-175</td>
<td rowspan="2">&gt; podoviridae <br />latent periods</td>
<td>CB0204, CB0202, CB0101</td>
<td>Wang (2007)</td>
</tr>
<tr>
<td>S-BB1</td>
<td>siphovirus</td>
<td>250</td>
<td>Synechococcus strain</td>
<td>Mann (2003)</td>
</tr>
</tbody>
</table>
*under phosphate limitation burst size is reduced by 80% and latent period is increased (Wilson et al. 1996)<br />NOTE: Estimates of burst sizes based on balancing estimates of viral decay and Synechococcus contacts yielded values ranging from 93 to 324 (Mann 2003)<br /><br /><a href="https://s3.amazonaws.com/pr-journal/pmc2ze.jpg" target="_blank"><img src="https://s3.amazonaws.com/pr-journal/pnc2ze.jpg" alt="" /></a><br /><br /><br /><strong>Table 1: </strong>Dilutions for Free Phage and Total Phage<br /><br />
<table border="0" cellspacing="1.5" cellpadding="3">
<tbody>
<tr>
<th colspan="5">Free Phage Dilutions</th>
<th colspan="5">Total Phage Dilutions</th>
</tr>
<tr>
<td>T0 FP 0</td>
<td>T0 FP -2</td>
<td>T0 FP -3</td>
<td> </td>
<td> </td>
<td>T0 FP 0</td>
<td>T0 FP -2</td>
<td>T0 FP -3</td>
<td> </td>
<td> </td>
</tr>
<tr>
<td>T1 FP 0</td>
<td>T1 FP -2</td>
<td>T1 FP -3</td>
<td> </td>
<td> </td>
<td>T1 FP 0</td>
<td>T1 FP -2</td>
<td>T1 FP -3</td>
<td> </td>
<td> </td>
</tr>
<tr>
<td>T2 FP 0</td>
<td>T2 FP -2</td>
<td>T2 FP -3</td>
<td> </td>
<td> </td>
<td>T2 FP 0</td>
<td>T2 FP -2</td>
<td>T2 FP -3</td>
<td> </td>
<td> </td>
</tr>
<tr>
<td>T3 FP 0</td>
<td>T3 FP -2</td>
<td>T3 FP -3</td>
<td> </td>
<td> </td>
<td>T3 FP 0</td>
<td>T3 FP -2</td>
<td>T3 FP -3</td>
<td> </td>
<td> </td>
</tr>
<tr>
<td>T3.5 FP 0</td>
<td>T3.5 FP -2</td>
<td>T3.5 FP -3</td>
<td> </td>
<td> </td>
<td>T3.5 FP 0</td>
<td>T3.5 FP -2</td>
<td>T3.5 FP -3</td>
<td> </td>
<td> </td>
</tr>
<tr>
<td>T4 FP 0</td>
<td>T4 FP -2</td>
<td>T4 FP -3</td>
<td> </td>
<td> </td>
<td>T4 FP 0</td>
<td>T4 FP -2</td>
<td>T4 FP -3</td>
<td> </td>
<td> </td>
</tr>
<tr>
<td>T4.5 FP 0</td>
<td>T4.5 FP -2</td>
<td>T4.5 FP -3</td>
<td> </td>
<td> </td>
<td>T4.5 FP 0</td>
<td>T4.5 FP -2</td>
<td>T4.5 FP -3</td>
<td> </td>
<td> </td>
</tr>
<tr>
<td>T5 FP 0</td>
<td>T5 FP -2</td>
<td>T5 FP -3</td>
<td>T5 FP -4</td>
<td> </td>
<td>T5 FP 0</td>
<td>T5 FP -2</td>
<td>T5 FP -3</td>
<td>T5 FP -4</td>
<td> </td>
</tr>
<tr>
<td>T5.5 FP 0</td>
<td>T5.5 FP -2</td>
<td>T5.5 FP -3</td>
<td>T5.5 FP -4</td>
<td> </td>
<td>T5.5 FP 0</td>
<td>T5.5 FP -2</td>
<td>T5.5 FP -3</td>
<td>T5.5 FP -4</td>
<td> </td>
</tr>
<tr>
<td>T6 FP 0</td>
<td>T6 FP -2</td>
<td>T6 FP -3</td>
<td>T6 FP -4</td>
<td> </td>
<td>T6 FP 0</td>
<td>T6 FP -2</td>
<td>T6 FP -3</td>
<td>T6 FP -4</td>
<td> </td>
</tr>
<tr>
<td>T6.5 FP 0</td>
<td>T6.5 FP -2</td>
<td>T6.5 FP -3</td>
<td>T6.5 FP -4</td>
<td> </td>
<td>T6.5 FP 0</td>
<td>T6.5 FP -2</td>
<td>T6.5 FP -3</td>
<td>T6.5 FP -4</td>
<td> </td>
</tr>
<tr>
<td>T7 FP 0</td>
<td>T7 FP -2</td>
<td>T7 FP -3</td>
<td>T7 FP -4</td>
<td> </td>
<td>T7 FP 0</td>
<td>T7 FP -2</td>
<td>T7 FP -3</td>
<td>T7 FP -4</td>
<td> </td>
</tr>
<tr>
<td>T7.5 FP 0</td>
<td>T7.5 FP -2</td>
<td>T7.5 FP -3</td>
<td>T7.5 FP -4</td>
<td> </td>
<td>T7.5 FP 0</td>
<td>T7.5 FP -2</td>
<td>T7.5 FP -3</td>
<td>T7.5 FP -4</td>
<td> </td>
</tr>
<tr>
<td>T8 FP 0</td>
<td>T8 FP -2</td>
<td>T8 FP -3</td>
<td>T8 FP -4</td>
<td> </td>
<td>T8 FP 0</td>
<td>T8 FP -2</td>
<td>T8 FP -3</td>
<td>T8 FP -4</td>
<td> </td>
</tr>
<tr>
<td>T8.5 FP 0</td>
<td>T8.5 FP -2</td>
<td>T8.5 FP -3</td>
<td>T8.5 FP -4</td>
<td> </td>
<td>T8.5 FP 0</td>
<td>T8.5 FP -2</td>
<td>T8.5 FP -3</td>
<td>T8.5 FP -4</td>
<td> </td>
</tr>
<tr>
<td>T9 FP 0</td>
<td>T9 FP -2</td>
<td>T9 FP -3</td>
<td>T9 FP -4</td>
<td> </td>
<td>T9 FP 0</td>
<td>T9 FP -2</td>
<td>T9 FP -3</td>
<td>T9 FP -4</td>
<td> </td>
</tr>
<tr>
<td>T9.5 FP 0</td>
<td>T9.5 FP -2</td>
<td>T9.5 FP -3</td>
<td>T9.5 FP -4</td>
<td> </td>
<td>T9.5 FP 0</td>
<td>T9.5 FP -2</td>
<td>T9.5 FP -3</td>
<td>T9.5 FP -4</td>
<td> </td>
</tr>
<tr>
<td>T10 FP 0</td>
<td>T10 FP -2</td>
<td>T10 FP -3</td>
<td>T10 FP -4</td>
<td>T10 FP -5</td>
<td>T10 FP 0</td>
<td>T10 FP -2</td>
<td>T10 FP -3</td>
<td>T10 FP -4</td>
<td>T10 FP -5</td>
</tr>
<tr>
<td>T10.5 FP 0</td>
<td>T10.5 FP -2</td>
<td>T10.5 FP -3</td>
<td>T10.5 FP -4</td>
<td>T10.5 FP -5</td>
<td>T10.5 FP 0</td>
<td>T10.5 FP -2</td>
<td>T10.5 FP -3</td>
<td>T10.5 FP -4</td>
<td>T10.5 FP -5</td>
</tr>
<tr>
<td>T11 FP 0</td>
<td>T11 FP -2</td>
<td>T11 FP -3</td>
<td>T11 FP -4</td>
<td>T11 FP -5</td>
<td>T11 FP 0</td>
<td>T11 FP -2</td>
<td>T11 FP -3</td>
<td>T11 FP -4</td>
<td>T11 FP -5</td>
</tr>
<tr>
<td>T11.5 FP 0</td>
<td>T11.5 FP -2</td>
<td>T11.5 FP -3</td>
<td>T11.5 FP -4</td>
<td>T11.5 FP -5</td>
<td>T11.5 FP 0</td>
<td>T11.5 FP -2</td>
<td>T11.5 FP -3</td>
<td>T11.5 FP -4</td>
<td>T11.5 FP -5</td>
</tr>
<tr>
<td>T12 FP 0</td>
<td>T12 FP -2</td>
<td>T12 FP -3</td>
<td>T12 FP -4</td>
<td>T12 FP -5</td>
<td>T12 FP 0</td>
<td>T12 FP -2</td>
<td>T12 FP -3</td>
<td>T12 FP -4</td>
<td>T12 FP -5</td>
</tr>
<tr>
<td>T12.5 FP 0</td>
<td>T12.5 FP -2</td>
<td>T12.5 FP -3</td>
<td>T12.5 FP -4</td>
<td>T12.5 FP -5</td>
<td>T12.5 FP 0</td>
<td>T12.5 FP -2</td>
<td>T12.5 FP -3</td>
<td>T12.5 FP -4</td>
<td>T12.5 FP -5</td>
</tr>
<tr>
<td>T13 FP 0</td>
<td>T13 FP -2</td>
<td>T13 FP -3</td>
<td>T13 FP -4</td>
<td>T13 FP -5</td>
<td>T13 FP 0</td>
<td>T13 FP -2</td>
<td>T13 FP -3</td>
<td>T13 FP -4</td>
<td>T13 FP -5</td>
</tr>
<tr>
<td>T13.5 FP 0</td>
<td>T13.5 FP -2</td>
<td>T13.5 FP -3</td>
<td>T13.5 FP -4</td>
<td>T13.5 FP -5</td>
<td>T13.5 FP 0</td>
<td>T13.5 FP -2</td>
<td>T13.5 FP -3</td>
<td>T13.5 FP -4</td>
<td>T13.5 FP -5</td>
</tr>
<tr>
<td>T14 FP 0</td>
<td>T14 FP -2</td>
<td>T14 FP -3</td>
<td>T14 FP -4</td>
<td>T14 FP -5</td>
<td>T14 FP 0</td>
<td>T14 FP -2</td>
<td>T14 FP -3</td>
<td>T14 FP -4</td>
<td>T14 FP -5</td>
</tr>
<tr>
<td>T14.5 FP 0</td>
<td>T14.5 FP -2</td>
<td>T14.5 FP -3</td>
<td>T14.5 FP -4</td>
<td>T14.5 FP -5</td>
<td>T14.5 FP 0</td>
<td>T14.5 FP -2</td>
<td>T14.5 FP -3</td>
<td>T14.5 FP -4</td>
<td>T14.5 FP -5</td>
</tr>
<tr>
<td>T15 FP 0</td>
<td>T15 FP -2</td>
<td>T15 FP -3</td>
<td>T15 FP -4</td>
<td>T15 FP -5</td>
<td>T15 FP 0</td>
<td>T15 FP -2</td>
<td>T15 FP -3</td>
<td>T15 FP -4</td>
<td>T15 FP -5</td>
</tr>
<tr>
<td>T15.5 FP 0</td>
<td>T15.5 FP -2</td>
<td>T15.5 FP -3</td>
<td>T15.5 FP -4</td>
<td>T15.5 FP -5</td>
<td>T15.5 FP 0</td>
<td>T15.5 FP -2</td>
<td>T15.5 FP -3</td>
<td>T15.5 FP -4</td>
<td>T15.5 FP -5</td>
</tr>
<tr>
<td>T16 FP 0</td>
<td>T16 FP -2</td>
<td>T16 FP -3</td>
<td>T16 FP -4</td>
<td>T16 FP -5</td>
<td>T16 FP 0</td>
<td>T16 FP -2</td>
<td>T16 FP -3</td>
<td>T16 FP -4</td>
<td>T16 FP -5</td>
</tr>
<tr>
<td>T24 FP 0</td>
<td>T24 FP -2</td>
<td>T24 FP -3</td>
<td>T24 FP -4</td>
<td>T24 FP -5</td>
<td>T24 FP 0</td>
<td>T24 FP -2</td>
<td>T24 FP -3</td>
<td>T24 FP -4</td>
<td>T24 FP -5</td>
</tr>
</tbody>
</table>
<br /><br /><a href="https://s3.amazonaws.com/pr-journal/phc2ze.jpg" target="_blank"><img src="https://s3.amazonaws.com/pr-journal/pic2ze.jpg" alt="" /></a><br /><br /><br /><span style="text-decoration: underline;"><strong>Serial Dilution:</strong></span><br /><br /><a href="https://s3.amazonaws.com/pr-journal/ppc2ze.jpg" target="_blank"><img src="https://s3.amazonaws.com/pr-journal/ppc2ze.jpg" alt="" /></a><br /><br /><br /><span style="text-decoration: underline;"><strong>Serial Dilution 2:</strong></span><br /><br /><a href="https://s3.amazonaws.com/pr-journal/pqc2ze.jpg" target="_blank"><img src="https://s3.amazonaws.com/pr-journal/prc2ze.jpg" alt="" /></a>

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