TITLE: Association between the rs1544410 polymorphism in the vitamin D receptor (VDR) gene and insulin secretion after gestational diabetes mellitus
AUTHORS: Nael Shaat, Anastasia Katsarou, Bushra Shahida, Rashmi B Prasad, Karl Kristensen, Tereza Planck

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:left"><a href="" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;">Genotyping using TaqMan allelic discrimination assay</span></a></div></div><div class = "text-block"><div class = "justify" style = "text-align:left"></div></div><div class = "text-block"><div class = "justify" style = "text-align:left"><span>Genotyping was done by adding 2 μl (total 10 ng) into MicroAmp™ Optical 384-well plate (Applied Biosystems, Sweden) and dried overnight. On the following day, a mixture of 2.5 µl of TaqMan® Genotyping Master Mix (Applied Biosciences, Sweden), 0.0625 µl of TaqMan® SNP Genotyping Assay </span><a href="" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;">(Applied Biosystems,</span></a><span> Sweden) and 2.4 µl of sterile H</span><span style = "vertical-align:sub;">2</span><span>O was added to the wells using a multichannel pipette. The plates were covered with MicroAmp™ optical Adhesive Film (Applied Biosystems, Sweden), vortexed and spun down shortly. Plates were then placed in a thermal cycler (Applied Biosystems, Sweden) with following PCR conditions: 50°C for 2 min, 95°C for 10 min and 40 cycles of (95°C for 15 sec and 60°C for 1 min). The plates were read using QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems, Sweden).TaqMan® SNP Genotyping Assay were designed using Custom Assay TaqMan® Design Tool (Applied Biosystems, Sweden). More details about the studied TaqMan® SNP are listed in the Table  below.</span></div></div><table border><tr style = "text-align:center;"><td>  </td><td>A</td><td>B</td><td>C</td><td>D</td></tr><tr><td style = "text-align:center;">1</td><td rowspan = "" colspan = "" style ="display : table-cell;">  <b style="mso-bidi-font-weight:normal">SNP</b>  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  <b>ID ThermoFischer</b>  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  <b style="mso-bidi-font-weight:normal">Gene</b>  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  <b style="mso-bidi-font-weight:normal">Position</b>  </td></tr><tr><td style = "text-align:center;">2</td><td rowspan = "" colspan = "" style ="display : table-cell;">  <b style="mso-bidi-font-weight:normal">rs731236</b>  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  C_2404008_10  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  VDR  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  Chr.12:
  47844974 on GRCh38  </td></tr><tr><td style = "text-align:center;">3</td><td rowspan = "" colspan = "" style ="display : table-cell;">  <b style="mso-bidi-font-weight:normal">rs7975232</b>  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  C_28977635_10     </td><td rowspan = "" colspan = "" style ="display : table-cell;">  VDR  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  Chr.12: 47845054 on GRCh38  </td></tr><tr><td style = "text-align:center;">4</td><td rowspan = "" colspan = "" style ="display : table-cell;">  <b style="mso-bidi-font-weight:normal">rs10735810/rs2228570</b>  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  C_12060045_20     </td><td rowspan = "" colspan = "" style ="display : table-cell;">  VDR  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  Chr.12: 47879112 on GRCh38  </td></tr><tr><td style = "text-align:center;">5</td><td rowspan = "" colspan = "" style ="display : table-cell;">  <b style="mso-bidi-font-weight:normal">rs1544410</b>  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  AHQJVHI  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  VDR  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  Chr.12: 47846052 on GRCh38  </td></tr><tr><td style = "text-align:center;">6</td><td rowspan = "" colspan = "" style ="display : table-cell;">  <b style="mso-bidi-font-weight:normal">rs7041</b>  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  C__3133594_30     </td><td rowspan = "" colspan = "" style ="display : table-cell;">  DBP  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  Chr.4: 71752617 on GRCh38  </td></tr><tr><td style = "text-align:center;">7</td><td rowspan = "" colspan = "" style ="display : table-cell;">  <b style="mso-bidi-font-weight:normal">rs4588</b>  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  C__8278879_10     </td><td rowspan = "" colspan = "" style ="display : table-cell;">  DBP  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  Chr.4: 71752606 on GRCh38  </td></tr><tr><td style = "text-align:center;">8</td><td rowspan = "" colspan = "" style ="display : table-cell;">  <b style="mso-bidi-font-weight:normal">rs10877012</b>  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  AHRSTNQ  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  CYP27B1  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  Chr.12: 57768302 on GRCh38  </td></tr><tr><td style = "text-align:center;">9</td><td rowspan = "" colspan = "" style ="display : table-cell;">  <b style="mso-bidi-font-weight:normal">rs4646536</b>  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  C_25623453_10     </td><td rowspan = "" colspan = "" style ="display : table-cell;">  CYP27B1  </td><td rowspan = "" colspan = "" style ="display : table-cell;">  Chr.12: 57764205 on GRCh38  </td></tr></table><div class = "text-block"><div class = "justify" style = "text-align:left"> </div></div><div class = "text-block"><div class = "justify" style = "text-align:justify"></div><style>
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