TITLE: No Title
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[DESCRIPTION]
<p>SpinOUT™ protocol for buffer exchange of peptide and protein samples.</p>
<p>(Cat. # 786-170 to 786-173, 786-703 to 786-708, 786-865 to 786-869)</p>

[GUIDELINES]
<p><strong>INTRODUCTION</strong></p>
<p>The SpinOUT™ columns are versatile, spin-format columns for the desalting and buffer exchange of peptide and protein and other macromolecule solutions ranging from 5µl through to 3ml sample volumes. The SpinOUT™ columns are available in three MWCO sizes for &gt;700, &gt;6,000 or &gt;30,000 dalton peptides or proteins and are suitable for samples containing as little as 20µg peptide or protein/ml. The</p>
<p> </p>
<p>SpinOUT™ columns are simple to use as the peptide or protein solution is applied and then centrifuged to recover protein with the column retaining &gt;95% of the salts and small molecules (&lt;100Da for SpinOUT™ GT-100, &lt;1,000Da for Spinout™ GT-600 and &lt;1,500 for Spinout™ GT-1200).</p>
<p> </p>
<p><strong>ITEMS SUPPLIED</strong></p>
<table style="width: 725px;">
<tbody>
<tr>
<td style="width: 73.15px;"><strong>Cat. #</strong></td>
<td style="width: 201.967px;"><strong>Description</strong></td>
<td style="width: 106px;"><strong># Supplied</strong></td>
<td style="width: 146.183px;"><strong>Resin Bed Volume (ml)</strong></td>
<td style="width: 162.3px;"><strong>Sample Load Volume (ml)</strong></td>
</tr>
<tr>
<td style="width: 73.15px;">786-865</td>
<td style="width: 201.967px;">SpinOUT™ GT-100, 0.1ml</td>
<td style="width: 106px;">25</td>
<td style="width: 146.183px;">0.1</td>
<td style="width: 162.3px;">0.005-0.03</td>
</tr>
<tr>
<td style="width: 73.15px;">786-866</td>
<td style="width: 201.967px;">SpinOUT™ GT-100, 1ml</td>
<td style="width: 106px;">10</td>
<td style="width: 146.183px;">1</td>
<td style="width: 162.3px;">0.03-0.3</td>
</tr>
<tr>
<td style="width: 73.15px;">786-867</td>
<td style="width: 201.967px;">SpinOUT™ GT-100, 3ml</td>
<td style="width: 106px;">10</td>
<td style="width: 146.183px;">3</td>
<td style="width: 162.3px;">0.3-0.9</td>
</tr>
<tr>
<td style="width: 73.15px;">786-868</td>
<td style="width: 201.967px;">SpinOUT™ GT-100, 5ml</td>
<td style="width: 106px;">5</td>
<td style="width: 146.183px;">5</td>
<td style="width: 162.3px;">0.75-1.5</td>
</tr>
<tr>
<td style="width: 73.15px;">786-869</td>
<td style="width: 201.967px;">SpinOUT™ GT-100, 10ml</td>
<td style="width: 106px;">5</td>
<td style="width: 146.183px;">10</td>
<td style="width: 162.3px;">1-3</td>
</tr>
<tr>
<td style="width: 73.15px;">786-703</td>
<td style="width: 201.967px;">SpinOUT™ GT-600, 0.1ml</td>
<td style="width: 106px;">25</td>
<td style="width: 146.183px;">0.1</td>
<td style="width: 162.3px;">0.005-0.03</td>
</tr>
<tr>
<td style="width: 73.15px;">786-170</td>
<td style="width: 201.967px;">SpinOUT™ GT-600, 1ml</td>
<td style="width: 106px;">10</td>
<td style="width: 146.183px;">1</td>
<td style="width: 162.3px;">0.03-0.3</td>
</tr>
<tr>
<td style="width: 73.15px;">786-171</td>
<td style="width: 201.967px;">SpinOUT™ GT-600, 3ml</td>
<td style="width: 106px;">10</td>
<td style="width: 146.183px;">3</td>
<td style="width: 162.3px;">0.3-0.9</td>
</tr>
<tr>
<td style="width: 73.15px;">786-704 </td>
<td style="width: 201.967px;">SpinOUT™ GT-600, 5ml </td>
<td style="width: 106px;">5 </td>
<td style="width: 146.183px;">5 </td>
<td style="width: 162.3px;">0.75-1.5 </td>
</tr>
<tr>
<td style="width: 73.15px;">786-705</td>
<td style="width: 201.967px;">SpinOUT™ GT-600, 10ml </td>
<td style="width: 106px;">5 </td>
<td style="width: 146.183px;">10 </td>
<td style="width: 162.3px;">1-3 </td>
</tr>
<tr>
<td style="width: 73.15px;">786-706 </td>
<td style="width: 201.967px;"> SpinOUT™ GT-1200, 0.1ml</td>
<td style="width: 106px;">25 </td>
<td style="width: 146.183px;">0.1 </td>
<td style="width: 162.3px;">0.005-0.03 </td>
</tr>
<tr>
<td style="width: 73.15px;">786-172</td>
<td style="width: 201.967px;"> SpinOUT™ GT-1200, 1ml</td>
<td style="width: 106px;">10 </td>
<td style="width: 146.183px;">1 </td>
<td style="width: 162.3px;">0.03-0.3 </td>
</tr>
<tr>
<td style="width: 73.15px;">786-173 </td>
<td style="width: 201.967px;"> SpinOUT™ GT-1200, 3ml</td>
<td style="width: 106px;">10 </td>
<td style="width: 146.183px;">3 </td>
<td style="width: 162.3px;">0.3-0.9 </td>
</tr>
<tr>
<td style="width: 73.15px;">786-707 </td>
<td style="width: 201.967px;">SpinOUT™ GT-1200, 5ml </td>
<td style="width: 106px;">5 </td>
<td style="width: 146.183px;">5 </td>
<td style="width: 162.3px;">0.75-1.5 </td>
</tr>
<tr>
<td style="width: 73.15px;">786-708</td>
<td style="width: 201.967px;">SpinOUT™ GT-1200, 10ml</td>
<td style="width: 106px;">5</td>
<td style="width: 146.183px;">10</td>
<td style="width: 162.3px;">1-3 </td>
</tr>
</tbody>
</table>
<p> </p>
<p><strong>STORAGE CONDITIONS</strong></p>
<p>The columns are shipped at ambient temperature. Upon arrival, store the columns at 4˚C. If stored and handled correctly the columns have a shelf-life of 1 year.</p>
<p> </p>
<p><strong>SPECIFICATIONS</strong></p>
<p><strong>SpinOUT™ GT-100</strong></p>
<p>• Particle size: 55-165µm</p>
<p>• Exclusion limit (Mr): 700</p>
<p><strong>SpinOUT™ GT-600</strong></p>
<p>• Particle size: 20-130µm</p>
<p>• Exclusion limit (Mr): 6,000</p>
<p><strong>SpinOUT™ GT-1200</strong></p>
<p>• Particle size: 35-200µm</p>
<p>• Exclusion limit (Mr): 30,000</p>
<p> </p>
<p><strong>IMPORTANT INFORMATION</strong></p>
<p><strong>Sample Load Volume</strong></p>
<p>The recommended load volumes (see table) are a guideline. The actual volumes used will be dependent on your sample, the concentration of salts and contaminants to be removed and the recovered purity desired. For optimal removal of contaminants, we recommend using a sample volume of &lt;20% of the resin bed volume.</p>
<p><strong>NOTE:</strong> Loading more than the recommended load volume will result in a higher level of contaminating salts and other molecules.</p>
<p> </p>
<p><strong>ADDITIONAL ITEMS NEEDED</strong></p>
<p>• Variable speed centrifuge</p>
<p>• 1.5-2ml microcentrifuge collection tubes for the 0.1ml (Cat. # 786-703, 786-706) and 1ml (Cat. # 786-170, 786-172) spin columns</p>
<p>• 15ml collection tubes for the 3ml (Cat. # 786-171, 786-173) and 5ml (Cat. # 786- 704, 786-707) spin columns</p>
<p>• 50ml collection tubes for the 10 ml (Cat. # 786-705, 786-708) spin columns</p>
<p>• Buffer for buffer-exchange</p>
<p> </p>
<p><strong>Table 1: Centrifugation times for optimal sample recovery.</strong></p>
<div class="step-content-block editable-content">
<table>
<tbody>
<tr>
<td>Column size</td>
<td>Centrifugation Time (mins)</td>
</tr>
<tr>
<td>0.1ml column</td>
<td>4</td>
</tr>
<tr>
<td>1ml column</td>
<td>4</td>
</tr>
<tr>
<td>3ml column</td>
<td>6</td>
</tr>
<tr>
<td>5ml column</td>
<td>8</td>
</tr>
<tr>
<td>10ml column</td>
<td>10 </td>
</tr>
</tbody>
</table>
</div>
<div> </div>

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