TITLE: No Title
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[DESCRIPTION]
<p>Defined Medium for Aureococcus anophagefferens</p>

[GUIDELINES]
<table>
<tbody>
<tr style="text-align: center;">
<td colspan="7">
<p><strong>Anhydrous Salts</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><strong>Constituent</strong></p>
</td>
<td>
<p><strong>Final Conc.</strong></p>
</td>
<td>
<p><strong>Molar Mass</strong></p>
</td>
<td>
<p><strong>g/1.0L</strong></p>
</td>
<td>
<p><strong>g/0.75L</strong></p>
</td>
<td>
<p><strong>g/0.5L</strong></p>
</td>
<td>
<p><strong>g/0.25L</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">NaCl</span></p>
</td>
<td>
<p><span style="font-weight: 400;">4.28E-01</span></p>
</td>
<td>
<p><span style="font-weight: 400;">58.44</span></p>
</td>
<td>
<p><span style="font-weight: 400;">25.01232</span></p>
</td>
<td>
<p><span style="font-weight: 400;">18.75924</span></p>
</td>
<td>
<p><span style="font-weight: 400;">12.50616</span></p>
</td>
<td>
<p><span style="font-weight: 400;">6.25308</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">KCl</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.10E-02</span></p>
</td>
<td>
<p><span style="font-weight: 400;">74.5513</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.8200643</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.615048225</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.410032</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.205016</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">Na2SO4</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.00E-02</span></p>
</td>
<td>
<p><span style="font-weight: 400;">142.04</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.4204</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.0653</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.7102</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.3551</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">NaHCO3</span></p>
</td>
<td>
<p><span style="font-weight: 400;">2.00E-03</span></p>
</td>
<td>
<p><span style="font-weight: 400;">84.007</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.168014</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.1260105</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.084007</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.042004</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td colspan="7">
<p><strong>Hydrous Salts</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><strong>Constituent</strong></p>
</td>
<td>
<p><strong>Final Conc.</strong></p>
</td>
<td>
<p><strong>Molar Mass</strong></p>
</td>
<td>
<p><strong>g/1.0L</strong></p>
</td>
<td>
<p><strong>g/0.75L</strong></p>
</td>
<td>
<p><strong>g/0.5L</strong></p>
</td>
<td>
<p><strong>g/0.25L</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">CaCl2·2H2O</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.25E-02</span></p>
</td>
<td>
<p><span style="font-weight: 400;">147.02</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.83775</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.3783125</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.918875</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.459438</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">MgCl2·6H2O</span></p>
</td>
<td>
<p><span style="font-weight: 400;">4.00E-02</span></p>
</td>
<td>
<p><span style="font-weight: 400;">203.3027</span></p>
</td>
<td>
<p><span style="font-weight: 400;">8.132108</span></p>
</td>
<td>
<p><span style="font-weight: 400;">6.099081</span></p>
</td>
<td>
<p><span style="font-weight: 400;">4.066054</span></p>
</td>
<td>
<p><span style="font-weight: 400;">2.033027</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td colspan="7">
<p><strong>Macronutrients</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><strong>Constituent</strong></p>
</td>
<td>
<p><strong>Final Conc.</strong></p>
</td>
<td>
<p><strong>Molar Mass</strong></p>
</td>
<td>
<p><strong>g/1.0L</strong></p>
</td>
<td>
<p><strong>g/0.75L</strong></p>
</td>
<td>
<p><strong>g/0.5L</strong></p>
</td>
<td>
<p><strong>g/0.25L</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">NaNO3</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.47E-03</span></p>
</td>
<td>
<p><span style="font-weight: 400;">84.9947</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.124942209</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.093706657</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.062471</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.031236</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">Ca2·glycerophosphate</span></p>
</td>
<td>
<p><span style="font-weight: 400;">2.00E-05</span></p>
</td>
<td>
<p><span style="font-weight: 400;">210.14</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.0042028</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.0031521</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.002101</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.001051</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">K2HPO4</span></p>
</td>
<td>
<p><span style="font-weight: 400;">4.00E-05</span></p>
</td>
<td>
<p><span style="font-weight: 400;">174.2</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.006968</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.005226</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.003484</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.001742</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td colspan="7">
<p><strong>Other</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><strong>Constituent</strong></p>
</td>
<td>
<p><strong>Final Conc.</strong></p>
</td>
<td>
<p><strong>Molar Mass</strong></p>
</td>
<td>
<p><strong>g/1.0L</strong></p>
</td>
<td>
<p><strong>g/0.75L</strong></p>
</td>
<td>
<p><strong>g/0.5L</strong></p>
</td>
<td>
<p><strong>g/0.25L</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">Tris Base</span></p>
</td>
<td>
<p><span style="font-weight: 400;">4.13E-03</span></p>
</td>
<td>
<p><span style="font-weight: 400;">121.14</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.5003082</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.37523115</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.250154</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.125077</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td colspan="7">
<p><strong>Stock Solutions</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><strong>Solution</strong></p>
</td>
<td>
<p><strong>Final Conc.</strong></p>
</td>
<td>
<p><strong>g/L</strong></p>
</td>
<td>
<p><strong>mL/1.0L</strong></p>
</td>
<td>
<p><strong>mL/0.75L</strong></p>
</td>
<td>
<p><strong>mL/0.5L</strong></p>
</td>
<td>
<p><strong>mL/0.25L</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">Fe-EDTA sodium Salt</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.79E-06</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.65702</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.75</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.5</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.25</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">TSM - I</span></p>
</td>
<td> </td>
<td> </td>
<td>
<p><span style="font-weight: 400;">1</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.75</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.5</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.25</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">TSM - II</span></p>
</td>
<td> </td>
<td> </td>
<td>
<p><span style="font-weight: 400;">1</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.75</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.5</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.25</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">TSM - III</span></p>
</td>
<td> </td>
<td> </td>
<td>
<p><span style="font-weight: 400;">1</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.75</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.5</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.25</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">S3 Vitamin Solution</span></p>
</td>
<td> </td>
<td> </td>
<td>
<p><span style="font-weight: 400;">1</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.75</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.5</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.25</span></p>
</td>
</tr>
<tr style="text-align: center;">
<td colspan="7">
<p><strong>Trace Metal Solution - TMS - I</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><strong>Constituent</strong></p>
</td>
<td>
<p><strong>Final Conc.</strong></p>
</td>
<td>
<p><strong>Molar Mass</strong></p>
</td>
<td>
<p><strong>g/1.0L 1000X Stock</strong></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">Na2EDTA·2H2O</span></p>
</td>
<td>
<p><span style="font-weight: 400;">8.53E-05</span></p>
</td>
<td>
<p><span style="font-weight: 400;">372.24</span></p>
</td>
<td>
<p><span style="font-weight: 400;">31.752072</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">FeCl3·6H2O</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.79E-06</span></p>
</td>
<td>
<p><span style="font-weight: 400;">270.3</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.483837</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">H3BO3</span></p>
</td>
<td>
<p><span style="font-weight: 400;">2.77E-04</span></p>
</td>
<td>
<p><span style="font-weight: 400;">61.83</span></p>
</td>
<td>
<p><span style="font-weight: 400;">17.12691</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">MnCl2·4H2O</span></p>
</td>
<td>
<p><span style="font-weight: 400;">9.10E-06</span></p>
</td>
<td>
<p><span style="font-weight: 400;">197.91</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.800981</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">ZnSO4·7H2O</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.07E-06</span></p>
</td>
<td>
<p><span style="font-weight: 400;">287.56</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.3076892</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">NaMoO4·2H2O</span></p>
</td>
<td>
<p><span style="font-weight: 400;">5.21E-06</span></p>
</td>
<td>
<p><span style="font-weight: 400;">218.9779</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.140874859</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">CoCl2·6H2O</span></p>
</td>
<td>
<p><span style="font-weight: 400;">2.21E-07</span></p>
</td>
<td>
<p><span style="font-weight: 400;">237.93</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.05258253</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td colspan="7">
<p><strong>Trace Metal Solution - TMS - II</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><strong>Constituent</strong></p>
</td>
<td>
<p><strong>Final Conc.</strong></p>
</td>
<td>
<p><strong>Molar Mass</strong></p>
</td>
<td>
<p><strong>g/1.0L 1000X Stock</strong></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">KBr</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.25E-04</span></p>
</td>
<td>
<p><span style="font-weight: 400;">119.002</span></p>
</td>
<td>
<p><span style="font-weight: 400;">14.87525</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">SrCl2 · 6H2O</span></p>
</td>
<td>
<p><span style="font-weight: 400;">3.42E-05</span></p>
</td>
<td>
<p><span style="font-weight: 400;">266.62</span></p>
</td>
<td>
<p><span style="font-weight: 400;">9.118404</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">RbCl</span></p>
</td>
<td>
<p><span style="font-weight: 400;">2.34E-06</span></p>
</td>
<td>
<p><span style="font-weight: 400;">120.92</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.2829528</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td colspan="7">
<p><strong>Trace Metal Solution - TMS - III</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><strong>Constituent</strong></p>
</td>
<td>
<p><strong>Final Conc.</strong></p>
</td>
<td>
<p><strong>Molar Mass</strong></p>
</td>
<td>
<p><strong>g/1.0L 1000X Stock</strong></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">Na2SeO3</span></p>
</td>
<td>
<p><span style="font-weight: 400;">2.00E-08</span></p>
</td>
<td>
<p><span style="font-weight: 400;">172.94</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.0034588</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">NaF</span></p>
</td>
<td>
<p><span style="font-weight: 400;">5.26E-05</span></p>
</td>
<td>
<p><span style="font-weight: 400;">41.98871</span></p>
</td>
<td>
<p><span style="font-weight: 400;">2.208606146</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">NaAsO2</span></p>
</td>
<td>
<p><span style="font-weight: 400;">4.00E-08</span></p>
</td>
<td>
<p><span style="font-weight: 400;">129.91</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.0051964</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">Al2(SO4)3·18H2O</span></p>
</td>
<td>
<p><span style="font-weight: 400;">1.11E-06</span></p>
</td>
<td>
<p><span style="font-weight: 400;">666.43</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.7397373</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">NH4VO3</span></p>
</td>
<td>
<p><span style="font-weight: 400;">2.00E-08</span></p>
</td>
<td>
<p><span style="font-weight: 400;">116.98</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.0023396</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td colspan="7">
<p><strong>S3 Vitamin Solution</strong></p>
</td>
</tr>
<tr style="text-align: center;">
<td>
<p><strong>Constituent</strong></p>
</td>
<td>
<p><strong>Final Conc.</strong></p>
</td>
<td>
<p><strong>Molar Mass</strong></p>
</td>
<td>
<p><strong>g/1.0L 1000X Stock</strong></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">Thiamine HCL</span></p>
</td>
<td>
<p><span style="font-weight: 400;">2.96E-07</span></p>
</td>
<td>
<p><span style="font-weight: 400;">337.27</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.09983192</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr style="text-align: center;">
<td>
<p><span style="font-weight: 400;">biotin</span></p>
</td>
<td>
<p><span style="font-weight: 400;">4.10E-09</span></p>
</td>
<td>
<p><span style="font-weight: 400;">244.31</span></p>
</td>
<td>
<p><span style="font-weight: 400;">0.001001671</span></p>
</td>
<td> </td>
<td> </td>
<td> </td>
</tr>
<tr>
<td style="text-align: center;">
<p><span style="font-weight: 400;">vitamin B12</span></p>
</td>
<td style="text-align: center;">
<p><span style="font-weight: 400;">7.40E-10</span></p>
</td>
<td style="text-align: center;">
<p><span style="font-weight: 400;">1.36E+03</span></p>
</td>
<td style="text-align: center;">
<p><span style="font-weight: 400;">0.0010064</span></p>
</td>
<td style="text-align: center;"> </td>
<td style="text-align: center;"> </td>
<td style="text-align: center;"> </td>
</tr>
</tbody>
</table>
<p> </p>

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