eamag/protocols-with-steps huggingface dataset:
TITLE: Mammalian Cell Nucleus Staining
AUTHORS: Kenneth Schackart, Kattika Kaarj

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Introductory protocol for cell nucelus staining using and imaging. This protocol uses Invitrogen™ NucBlue™ LiveReady Probes™.</div></div>

[STEPS]
?. [Stain Cells]
Using micropipette, remove cell culture media from each well.
?. [Stain Cells]
Wash each well twice with DPBS.
100 µl
?. [Stain Cells]
Add NucBlue™ solution to each well.
100 µl
?. [Stain Cells]
Cover with aluminum foil.
The aluminum foil is simply to prevent photobleaching of the fluorescent dye.
?. [Stain Cells]
Incubate for
TITLE: Viral purification from faecal sample
AUTHORS: sarah.schulz

[DESCRIPTION]
Protocol for the purification of viral particles from a faecal sample

[STEPS]
1. Homogenise 200 mg of faecal sample in 30 ml of SM buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl, 8 mM MgSO4)
2. Centrifuge sample at 4000 rpm for 20 min, then filter through a 0.2 μm filter
3. Add filtrate to a 100-kDa-molecular-mass Amicon Ultra-15 Centrifugal Filter
4. Centrifuge filter tube at 4000 rpm for 3 min to concentrate sample
5. Repeat centrifugation until the entire diluted filtrate has been passed through the filter tube
6. Dilute filtrate with 30 ml SM buffer 
7. Add diluted filtrate to a fresh 100-kDa-molecular-mass Amicon Ultra-15 Centrifugal Filter
8. Repeat centrifugation until a filtrate volume of at least 500 μl is reached
9. Add 10 U of DNase I and 10 U of RNase to the filtrate and incubate at 37°C for 30 min
10. Incubate samples at 65°C for 15 min to inactivate enzymes
11. Store at 4°C
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This protocol is used to dissociate adult (8-10 week) mouse testis into a single cell suspension. The entire procedure is carried out 'on ice', reducing artifact gene expression changes.  The yield is 5.4 million cells from 25 mg tissue and the viability is ~99%. </p>

[GUIDELINES]
<p><strong><u>Reagents</u></strong></p>
<p>Collagenase Type 4 – Worthington (LS004186)</p>
<p>Soybean Trypsin Inhibitor – Roche (10109886001) - 100 µL aliquots of 1 mg/mL made up in DPBS.</p>
<p>DNAse - Applichem (A3778) - 10 µL aliquots in DPBS each with 250 U</p>
<p>DPBS (no Ca, no Mg) - ThermoFisher (14190144)</p>
<p>Bovine Serum Albumin - Sigma (A8806)</p>
<p><strong><u> </u></strong></p>
<p><strong><u>Collagenase Enzyme Mix (1 mL)</u></strong></p>
<p>10 mg Coll. Type 4</p>
<p>100 µL of 1 mg/mL Soybean Trypsin Inhibitor (100 µg)</p>
<p>5 µl Dnase I (125 U/mL)</p>
<p>895µl DPBS </p>
<p> </p>
<p><strong><u>Equipment</u></strong></p>
<p>gentleMACS dissociator (130-093-235) + gentleMACS C-tubes (130-093-237)</p>
<p>Centrifuge for 1.5 mL, 15 mL conicals<br /> Pipettes and pipet tips<br /> 15, 50 ml Conicals (MLS)<br /> 1.5 mL tubes (MLS)<br /> 30 µM filters - Miltenyi (130-098-458) <br /> Petri dishes (MLS)<br /> Razor blades (MLS)<br /> Ice bucket w/ice<br /> Hemocytometers - InCyto Neubauer Improved (DHC-NO1-5)</p>
<p> </p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Pioneer Woman CBD Gummies Reviews: Best Gummy Bears In USA
AUTHORS: pioneerwomangummireviews  

[DESCRIPTION]
Pioneer Woman CBD Gummies Reviews: Best Gummy Bears In USA


[STEPS]
1. Pioneer Woman CBD Gummies Reviews: Best Gummy Bears In USA
Pressure, despair, concern, and a variety of other internal issues face people with a lot of liabilities, and they must be dealt with on time and in a natural way. The brain is vital to a person’s overall physical well- being, but when neurodegenerative ails strike, the entire body’s effectiveness suffers. Feting the graveness of the problem, Ree Drummond created Ree Drummond CBD Gummies as a mending cure for you. It’s great for common inflammation, excruciating reverse pain, and other common pangs and pains that are each too common these days. Pioneer Woman CBD Gummies have come one of the most popular brain safety, fitness, and heartiness supplements. 
Ree Drummond CBD Gummies are well- known for their capability to ameliorate general health and treat a variety of conditions, including stress, melancholy, anxiety, internal pain, seizures, and more. This is a CBD comestible result that's meant to help guests with their fleshly pangs and pains in a natural way. These delicacies contain no fungicides or dangerous constituents, according to the sanctioned website, and they help druggies relax. These CBD sweets vary from regular bears since they contain shops prize. 
There are substantially advantages and further that you'll get from Ree Drummond CBD Gummies Reviews but without important effect. No drug is needed or regular checks and it's 100 natural concentrate. 
About Pioneer Woman CBD Gummies 
Ree Drummond Or Pioneer Woman CBD Gummies are a fantastic product for pain relief. It’s also an anti-inflammatory, which means it’ll get relieve of your habitual pain for good. still, it's substantially used to treat and reduce common pain. It also addresses common suffering issues similar as shoulder pain, common discomfort, knee vexation, and so on, as well as the issues that go on with them, similar as anxiety and concern, internal stress, and physical aggravation, all of which contribute to wakefulness. This product is made from organically tended, high- quality hemp grown fully in the United States. 

Official Website:- http://healthynutripills.com/pioneer-woman-cbd-gummies-reviews-scam-report/
https://www.facebook.com/Pioneer-Woman-CBD-Gummies-101840469247086
https://sites.google.com/view/pioneer-woman-cbd-gummies-re/home
https://pioneer-woman-cbd-gummies-reviews-1.jimdosite.com/
https://in.pinterest.com/pin/777222848208008372/
https://twitter.com/WomanCbd
https://www.bonfire.com/pioneer-woman-cbd-gummies-reviewws/
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.</p>
<p> </p>
<p>Modified from Bold 1949, Bischoff and Bold 1963</p>
<p> </p>
<p> </p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
This is the protocol for&nbsp;Protein Expression Using BL21(DE3) Competent E. coli (C2527).&nbsp;For large scale, please follow&nbsp;<a href="https://protocols.io/view/Protein-Expression-Using-BL21-DE3-Large-Scale-C252-imsum5" target="_blank">this</a>&nbsp;protocol variant.

[GUIDELINES]
<strong>BL21(DE3)&nbsp;Genotype:</strong><br />fhuA2 [lon] ompT gal (&lambda; DE3) [dcm] ∆hsdS<br />&lambda; DE3 = &lambda; sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7&nbsp;gene1) i21 ∆nin5<br /><br /><strong>NOTES:</strong><br />1. <strong>Caution:&nbsp;</strong>This product contains DMSO, a hazardous material. Review the MSDS before handling.<br /><br />2. <strong>Storage and Handling</strong>:&nbsp;Competent cells should be stored at -80&deg;C. Storage at -20&deg;C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80&deg;C, even if they do not thaw.

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
Integrated Whole Exome Sequencing (WES) analysis pipline using various tools and databases, developed as part of the Accelerator program for Discovery in Brain disorders using Stem cells (ADBS) program at National Centre for Biological Sciences (NCBS), Bangalore.

[STEPS]
SECTION: Define paths and directories
?. 
SECTION: Unzip the raw reads files from .gz to fastq format
?. 
SECTION: QC check of R1 and R2 paired-end raw reads using FASTQC, Trimming poor quality reads using Prinseq-lite, and Adapter contimination removal using AfterQC,
?. 
SECTION: Alignment of clened raw reads against Human Reference Genome hg19 GRCh37.p13 build using BWA and SAMTOOLS.
?. 
SECTION: Mark PCR duplicates and sorting BAM using PICARD Tools
?. 
SECTION: Index the coordinate sorted bam file using SAMTOOLS
?. 
SECTION: INDEL re-alignment using GATK tools
?. 
SECTION: Check the alignment QC of the bam file using Qualimap
?. 
SECTION: SNP and INDEL variant calling using VarScan and SAMTOOLS
?. 
SECTION: VCF QC of SNP and INDEL files using rtg-tools
?. 
SECTION: SNP AND INDEL variant annotation using ANNOVAR
?. 
SECTION: Delete inter-mediate files after varifying the final results files (OPTIONAL STEP AS PER USER)
?.
TITLE: Sequencing open chromatin of single cell nuclei: snATAC-seq
AUTHORS: Sebastian Preissl, Xinxin Wang, Bing Ren

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Protocol for applying the TN5-based ATAC-seq method to the analysis of the chromatin of single cell nuclei isolated from brain tissue, as performed within the Center for Epigenomics of the Mouse Bain Atlas (CEMBA) and in coordination with the BRAIN Initiative Cell Census Network (BICCN).</div></div>

[STEPS]
TITLE: Fixation Protocol for Fresh Frozen Tissue Samples (post-MALDI)
AUTHORS: Elizabeth Neumann, Jamie Allen, Jeff Spraggins

[DESCRIPTION]
Scope:
To provide a method for removal of MALDI matrix, fixation and preparation of tissue sections for Cell DIVE.

[GUIDELINES]
Special Notes:
1. Make sure no tissue drying, particularly after matrix removal.
2. Glass petri dishes can be used for more fragile tissues.
3. This protocol can be used on Cell DIVE fresh frozen samples.

[STEPS]
1. After the tissue has been imaged via MALDI IMS, the slide should be placed in a coplin jar or glass petri dish with a solution of room temperature 4% paraformaldehyde in PBS (*must be room temperature).
2. Place jar/dish on a shaker for 5 minutes.
3. Discard the PFA (the MALDI IMS matrix will come off the slide in this step).
4. Add fresh 4% PFA in PBS and shake for 5 more minutes (for a total of 10 minutes in PFA).

 Washing with PFA solution will fix and rehydrate the tissue as the matrix is removed.  This preserves the tissue and antigens, and significantly reduces artifacts.
5. Add fresh PBS for 3 minutes on shaker. 
Repeat this wash two more times for a total of 3 times for 3 minutes each.
 pH should be neutral
6. Incubate the slides in 50 mg/mL bovie serum albumin (BSA), 10% (v/v) host-derived serum (depends on which antibodies they use), and 0.5% (v/v) Tween-20 in PBS for 30 minutes.
7. The tissue should be placed in PBS for 3 minutes on a shaker.  
Repeat this wash two more times for a total of 3 times for 3 minutes each.
6.1. Be very careful. Tissue will be very fragile.
This permeabilizes the tissue slowly. 
The Tween cannot be substituted for triton X, as triton X is too harsh and will degrade the tissue. 
30 minutes works for most.  Can do up to 2 hours before the tissue gets very damaged/adherence issues/weird background. 
7.1. Ensure the donkey serum is reconstituted (10 mL of ddH20)

2.5 grams of BSA
5 mL of reconstituted donkey serum
0.25 mL of Tween 20
44.75 mL of 1X PBS
---------------------------
50 mL block solution

Be very careful. Tissue will       be very fragile.
This permeabilizes the tissue       slowly. The Tween cannot be substituted for triton X, as triton X is too       harsh and will degrade the tissue. 
30 minutes works for most.       Can do up to 2 hours before the tissue gets very damaged/adherence       issues/weird background. 
6.1. 
7. Add fresh PBS for 3 minutes on shaker. 
Repeat this wash two more times for a total of 3 times for 3 minutes each.
TITLE: Preparing plasmids for nucleofection  of hPSCs
AUTHORS: Hanqin  Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner

[DESCRIPTION]
This protocol describes the standard procedure for the preparation of plasmids to be delivered into human pluripotent stem cells (hPSCs) using nucleofection.

General notes
1. Throughout this protocol, the term hPSC is used to collectively refer to both hiPSCs and hESCs. All described procedures have been tested and work equally well for hiPSCs and hESCs.



[STEPS]
1. Thaw plasmids on ice 

2. In each nucleofection, use 1 µg total of plasmid
3. For prime editing PE2 strategy, use:
    500 ng pCMV-PE2
    500 ng pU6-pegRNA
4. For prime editing PE3 strategy, use:
    500 ng pCMV-PE2
    330 ng pU6-pegRNA
    170 ng pBPK1520-ngRNA
5. Pipet the proper amount of each plasmid into a microcentrifuge tube. If the volume is too small, dilute in autoclaved water first.
TITLE: Descending Platform
AUTHORS: daniel.dautan, Per Svenningsson

[DESCRIPTION]
Behavioral test to assess motor function.

[STEPS]
1. Place the custom-made horizontal grid (0.5cm space between grid, 45cm long, 5cm wide) to have an angle of 45 degrees with the floor.
2. A clean cage was placed at the bottom of the grid.
3. Place a camera on top of the grid to allow recording of the time to descend.
 
4. Naïve mice were placed on the horizontal end of the grid and gently pushed to descend the grid towards the cage. 
5. Quantification: 
Time to descend the first and second half of the grid
Using the video and a proper scale, the distance between the hindlimbs of the mice was determined at 5 randomly selected frames and used as average.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
Protocol for digestion with NEBNext dsDNA Fragmentase (M0348)

[BEFORE_START]
Adequate mixing of NEBNext dsDNA Fragmentase&nbsp;is important for the success of this reaction.&nbsp;NEBNext dsDNA Fragmentase should be vortexed for&nbsp;3 seconds prior to use.<br /><br />The protocol listed below is for fragmentation of&nbsp;5 ng&ndash;3 &mu;g of DNA.

[GUIDELINES]
<p>Adequate mixing of NEBNext dsDNA Fragmentase is important for the success of this reaction. NEBNext dsDNA Fragmentase should be vortexed for 3 seconds prior to use.<br /><br />For tough digestions, add 1 μl of 200 mM MgCl2 to the reaction. Additional MgCl2 can be added if necessary.<br /><br />The protocol listed below is for fragmentation of 5 ng–3 μg of DNA.<br /><br /></p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Optimus GelРецензије - састав - *Прочитајте масти* Где купити!
AUTHORS: Optimus Gel

[DESCRIPTION]
Оптимус Гел је нови квалитетан производ који обједињује најновија научна достигнућа која заувек ублажавају и отклањају болове у зглобовима, костима и мишићима! Наручите данас са 50% попуста!
https://www.digitalholic.today/ba/optimus-gel-recenzije/

[STEPS]
TITLE: Protocol for Generation of Pre-Formed Fibrils from Alpha-Synuclein Monomer
AUTHORS: The Michael J Fox Foundation Pff Standardization Consortium

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a consensus protocol developed through discussions with Laura Volpicelli-Daley, Caryl Sortwell, Kelvin Luk, Lindsey Gottler, and Virginia Lee. This protocol is intended for research purposes only, using specially-formulated monomeric alpha-synuclein protein available for purchase at Proteos, Inc as the result of efforts by The Michael J. Fox Foundation (MJFF). Each batch of the “Alpha-Synuclein Monomer Protein for Making Pre- Formed Fibrils” has undergone internal purification and quality control at Proteos in addition to external validation to confirm successful generation of pathogenic aSyn PFFs. See Reference section for methods and results from application of alpha-synuclein pre-formed fibrils (aSyn PFFs) in primary neuron cultures in vitro or in mice in vivo. This protocol is referenced in the Polinski et al 2018 paper entitled "Best Practices for Generating and Using Alpha-Synuclein Pre-Formed Fibrils to Model Parkinson's Disease in Rodents" (doi: 10.3233/JPD-171248).</div></div>

[STEPS]
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
Mapping metagenomic reads from <a href="https://github.com/MicroB3-IS/osd-analysis" target="_blank">Ocean Sampling Da</a>y (OSD) 2014 against NCBI's ViralRefSeq alongside viral sequences identified from the Tara Oceans survey using <a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4533152/" target="_blank">VirSorter</a>.

[BEFORE_START]
<p>To run this protocol, users must first <a href="https://user.iplantcollaborative.org/register/" target="_blank">register</a> for Cyverse account. All data (both inputs and outputs) are available within Cyverse's data store at /iplant/home/shared/iVirus/ExampleData/</p>
<p> </p>
<p>All source code is available at the Sullivan lab bitbucket repository, <a href="https://bitbucket.org/MAVERICLab/" target="_blank">MAVERICLab</a> - either *-bowtiebatch or *-read2refmapper. * denotes either docker or stampede-based repos.</p>

[GUIDELINES]
One of the most commonly used procedures for analyzing viral metagenomic data is to map their reads (or reads from another dataset) against a set of references, often those from the read assembly. For example, if one wanted to know how well-represented viruses in NCBI's Viral Reference Sequences (ViralRefSeq) were in ocean viromes, they could map reads from lots of ocean viral metagenomes against ViralRefSeq. This is generally done using <a href="http://bowtie-bio.sourceforge.net/bowtie2/index.shtml" target="_blank">Bowtie2</a> or <a href="http://bio-bwa.sourceforge.net/" target="_blank">BWA</a>, by selecting a reference set of sequences, and then providing paired or unpaired reads to Bowtie2/BWA. <em>Then</em> the results must be processed/filtered to generate coverage tables. Dealing with setting up multiple reads files (10 paired metagenomes = 10 alignment runs) and the processing those read files can be challenging (not to mention requring computational resources).<br /><br />In this protocol, we'll be using reads fromÂ <a href="https://github.com/MicroB3-IS/osd-analysis" target="_blank">Ocean Sampling Da</a>y (OSD) 2014 and map them against ViralRefSeq alongside viral sequences identified from the Tara Oceans survey using <a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4533152/" target="_blank">VirSorter</a>.

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Sea Water - Blue Treasure
AUTHORS: Willian Barela Costa

[DESCRIPTION]
Pt  🇧🇷

Produção de água do mar para cultivo de microalgas utilizando a marca Blue Treasure.

25 - 35 Salinidade


En

Sea water production for microalgae cultivation using the Blue Treasure supplier. 

25 - 35 Salinity

27 mg/mL 



[STEPS]
SECTION: Salting process - 1 liter
4. 0.027 g/mL ->   27 g Sea Sal to 1 L     

SECTION: Salting process - 1 liter
5. Shake until diluted
SECTION: Salting process - 1 liter
6. Make sure the salinity is within the desired range using the salinity refractometer. In this protocol, the focus is 27 salinity.
SECTION: Metodologia - 1 litro
1. 0.027 g/mL ->   27 g Sal do mar para 1 L     

SECTION: Metodologia - 1 litro
2. Agite até diluir
SECTION: Metodologia - 1 litro
3. Certifique-se de que a salinidade esteja dentro da faixa desejada usando o refratômetro de salinidade. Neste protocolo, o foco é a salinidade 27.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>The cells targeted by the Streptavidin Nanobeads are either selected or depleted by incubating your sample with the magnetic particles after incubating with a biotin-conjugated antibody or antibody cocktail. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.</p>

[GUIDELINES]
<p><strong>Protocol:</strong></p>
<p>This procedure is optimized for the isolation of 10<sup>7</sup> to 2 x 10<sup>8</sup> cells per tube. If working with fewer than 10<sup>7</sup> cells, keep volumes as indicated for 10<sup>7</sup> cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.</p>
<p> </p>
<p> </p>
<p><strong>Reagents and instruments required:</strong><br />-MojoSort™ Buffer (5X) (Cat. No. 480017)<br />-MojoSort™ Magnet (Cat. No. 480019) or compatible magnetic separation system<br />-Adjustable pipettes<br />-70 μm filters (one per sample)<br />-5 mL (12 x 75 mm) polystyrene tubes<br />-Reagents for sample preparation<br />-Reagents and instruments (Flow cytometer) to determine yield and purity</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Türk solution and Trypan Blue
AUTHORS: Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This recepe is used in the following protocols:</div><div class = "text-block">- Separation and purification of human PBMC from FRESH BLOOD</div><div class = "text-block">- Separation and purification of human PBMC from BUFFY COAT</div><div class = "text-block">- Magnetic bead-based CD4+ T cell isolation from PBMCs with Dynabeads: CD4 Positive Isolation Kit</div><div class = "text-block">- Magnetic bead-based TREG-TEFF cell isolation from PBMC with Miltenyi CD4+CD25+ Regulatory T cell Isolation Kit</div><div class = "text-block">- Separation of Human Neutrophils (PMN) from Buffy Coat</div><div class = "text-block">- Separation of Human Neutrophils (PMN) from Whole Blood</div></div>

[STEPS]
TITLE: Flow cytometry for ex vivo stimulated pMacs
AUTHORS: Rebecca Wallings

[DESCRIPTION]
Flow cytometry for ex vivo stimulated pMacs

[STEPS]
1. Incubate cells for 30 minutes at 37 degrees in incubator in media containing 3mM EDTA at pH 6.2 to gently lift cells from plate. To encourage dissociation from plate, gently tap plate, or alternatively use a mini scraper
2. Aliquot cells in to wells of v-bottom 96 well plate

Spin cells at 300 x g at 4 degrees for 5 minutes

Resuspend cells in 50uL of antibody cocktail containing live/dead dye and Fc block

Incubate at 4 degrees in the dark for 20 minutes

Spin cells at 300 x g at 4 degrees for 5 minutes

Remove supernatant and resuspend pellet in 200uL 1 x PBS

Spin cells at 300 x g at 4 degrees for 5 minutes

Remove supernatant and resuspend pellet in 200uL 1 x PBS

Spin cells at 300 x g at 4 degrees for 5 minutes

Remove supernatant and resuspend pellet in 50uL ICC fixation buffer (Invitrogen)

Incubate cells at room temperature for 10 minutes in the dark

Spin cells at 300 x g at 4 degrees for 5 minutes

Remove supernatant and resuspend pellet in 200uL FACS buffer and proceed to assess cells on cytometer
TITLE: Protocol-RT-qPCR
AUTHORS: Klaus Hirschbühl

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><div class = "justify" style = "text-align:center">Protocol-RT-qPCR to the paper:</div></div><div class = "text-block"><div class = "justify" style = "text-align:center"></div></div><div class = "text-block"><div class = "justify" style = "text-align:center"><span style = "font-weight:bold;">Viral mapping in COVID-19 deceased in the Augsburg autopsy series of the first wave: a multiorgan and multimethodological approach</span></div></div><div class = "text-block"><div class = "justify" style = "text-align:center"><span style = "font-weight:bold;">Full organ mapping of patients with COVID-19: an autopsy study</span></div></div></div>

[STEPS]