TITLE: Protocol for preparing minced Artemia nauplii
AUTHORS: Ranjith  Lakshmanan, Linga Prabu Dhanasekaran, Kalidas C, Mathanbabu   A, Saravanan Raju

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Protocol for preparing minced </span><span style = "font-weight:bold;font-style:italic;">Artemia </span><span style = "font-weight:bold;">napulii as feed for young medusa of upside-down jellyfish </span></div><div class = "text-block"><span style = "font-weight:bold;font-style:italic;">Cassiopea andromeda</span></div><div class = "text-block"><ul style = "list-style-type:disc;"><li style = "counter-reset:ol0;list-style-type:disc;"><span>Laboratory hatched </span><span style = "font-style:italic;">Artemia </span><span>nauplii (Instar I & II stage) were filtered using a 250-micron sieve and concentrated to </span><a href="#" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;"> </span></a><span> volume.</span></li><li style = "counter-reset:ol0;list-style-type:disc;"></li><li style = "counter-reset:ol0;list-style-type:disc;"><span>The </span><span style = "font-style:italic;">Artemia </span><span>nauplii concentrate were centrifuging @ 8000 rpm for 3 minutes.</span></li><li style = "counter-reset:ol0;list-style-type:disc;"></li><li style = "counter-reset:ol0;list-style-type:disc;"><span>The supernatant was removed and </span><span style = "font-style:italic;">Artemia </span><span>nauplii biomass was macerated using a sterile plastic pestle. </span></li><li style = "counter-reset:ol0;list-style-type:disc;"></li><li style = "counter-reset:ol0;list-style-type:disc;"><span>Completely minced </span><span style = "font-style:italic;">Artemia </span><span>biomass was homogenously mixed with 5 mL of distilled water to make the concentration of 900-1100 nauplii/mL.</span></li><li style = "counter-reset:ol0;list-style-type:disc;"></li><li style = "counter-reset:ol0;list-style-type:disc;"><span>Two drops (</span><a href="#" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;"> </span></a><span> ) of minced </span><span style = "font-style:italic;">Artemia </span><span>biomass was fed to young medusa. </span></li></ul></div><div class = "text-block"><ul style = "list-style-type:disc;"><li style = "counter-reset:ol0;list-style-type:disc;"><span>The </span><span style = "font-style:italic;">Artemia </span><span>nauplii concentrate were centrifuging @ 8000 rpm for 3 minutes.</span></li><li style = "counter-reset:ol0;list-style-type:disc;"></li><li style = "counter-reset:ol0;list-style-type:disc;"><span>The supernatant was removed and </span><span style = "font-style:italic;">Artemia </span><span>nauplii biomass was macerated using a sterile plastic pestle. </span></li><li style = "counter-reset:ol0;list-style-type:disc;"></li><li style = "counter-reset:ol0;list-style-type:disc;"><span>Completely minced </span><span style = "font-style:italic;">Artemia </span><span>biomass was homogenously mixed with 5 mL of distilled water to make the concentration of 900-1100 nauplii/mL.</span></li><li style = "counter-reset:ol0;list-style-type:disc;"></li><li style = "counter-reset:ol0;list-style-type:disc;"><span>Two drops (</span><a href="#" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;"> </span></a><span> ) of minced </span><span style = "font-style:italic;">Artemia </span><span>biomass was fed to young medusa. </span></li></ul></div><div class = "text-block"><ul style = "list-style-type:disc;"><li style = "counter-reset:ol0;list-style-type:disc;"><span>The supernatant was removed and </span><span style = "font-style:italic;">Artemia </span><span>nauplii biomass was macerated using a sterile plastic pestle. </span></li><li style = "counter-reset:ol0;list-style-type:disc;"></li><li style = "counter-reset:ol0;list-style-type:disc;"><span>Completely minced </span><span style = "font-style:italic;">Artemia </span><span>biomass was homogenously mixed with 5 mL of distilled water to make the concentration of 900-1100 nauplii/mL.</span></li><li style = "counter-reset:ol0;list-style-type:disc;"></li><li style = "counter-reset:ol0;list-style-type:disc;"><span>Two drops (</span><a href="#" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;"> </span></a><span> ) of minced </span><span style = "font-style:italic;">Artemia </span><span>biomass was fed to young medusa. </span></li></ul></div><div class = "text-block"><ul style = "list-style-type:disc;"><li style = "counter-reset:ol0;list-style-type:disc;"><span>Completely minced </span><span style = "font-style:italic;">Artemia </span><span>biomass was homogenously mixed with 5 mL of distilled water to make the concentration of 900-1100 nauplii/mL.</span></li><li style = "counter-reset:ol0;list-style-type:disc;"></li><li style = "counter-reset:ol0;list-style-type:disc;"><span>Two drops (</span><a href="#" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;"> </span></a><span> ) of minced </span><span style = "font-style:italic;">Artemia </span><span>biomass was fed to young medusa. </span></li></ul></div><div class = "text-block"><ul style = "list-style-type:disc;"></ul></div></div>

[STEPS]