TITLE: WHO-recommended handrub formulations
AUTHORS: WHO

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Composition of alcohol-based formulations for in-house/local production </span></div><div class = "text-block">The choice of components for WHO handrubs takes into account both cost constraints and microbiological efficacy. The procurement of raw ingredients will be influenced by the availability of sub-standard materials on the market and it is important to select local sources with care. </div><div class = "text-block">The following two alcohol-based handrub formulations are recommended for preparation in-house or in a local production facility, up to a maximum of 50 litres: </div><div class = "text-block"><span style = "font-weight:bold;">Formulation 1 </span></div><div class = "text-block">To produce final concentrations of ethanol 80% v/v, glycerol 1.45% v/v, hydrogen peroxide (H2O2) 0.125% v/v. </div><div class = "text-block"><span style = "font-weight:bold;">Formulation 2</span><span style = "font-weight:bold;"> </span></div><div class = "text-block">To produce final concentrations of isopropyl alcohol 75% v/v, glycerol 1.45% v/v, hydrogen peroxide (H2O2) 0.125% v/v: Only pharmacopoeial quality reagents should be used (e.g. The International Pharmacopoeia) and not technical grade products</div></div>

[STEPS]
?. Method for  preparations
10 L
These can be prepared in 10-liter glass or plastic bottles with screw-threaded stoppers.
?. Pour  into the large bottle or tank up to the graduated mark.
[Ethanol 96%]
?. Add  using the measuring cylinder.
[Hydrogen peroxide 3%]
?. Add  using a measuring cylinder.
[Glycerol]
As glycerol is very viscous and sticks to the wall of the measuring cylinder, it should be rinsed with some sterile distilled or cold boiled water and then emptied into the bottle/tank.
?. Top the bottle/tank up to the 10-liter mark with sterile distilled or cold boiled water.
?. Place the lid or the screw cap on the tank/bottle as soon as possible after preparation, in order to prevent evaporation.
?. Mix the solution by shaking gently where appropriate or by using a paddle.
?. Immediately divide up the solution into its final containers.
E.g. 500 or 100 ml plastic bottles.
?. Pour  into the large bottle or tank up to the graduated mark.
[Isopropyl alcohol 99.8%]
?. Add  using the measuring cylinder.
[Hydrogen peroxide 3%]
?. Add  using a measuring cylinder.
[Glycerol]
As glycerol is very viscous and sticks to the wall of the measuring cylinder, it should be rinsed with some sterile distilled or cold boiled water and then emptied into the bottle/tank.
?. Top the bottle/tank up to the 10-liter mark with sterile distilled or cold boiled water.
?. Place the lid or the screw cap on the tank/bottle as soon as possible after preparation, in order to prevent evaporation.
?. Mix the solution by shaking gently where appropriate or by using a paddle.
?. Place the bottles in quarantine for  before use.
This allows time for any spores present in the alcohol or the new/re-used bottles to be destroyed.
?. Immediately divide up the solution into its final containers.
E.g. 500 or 100 ml plastic bottles.
?. Place the bottles in quarantine for  before use.
This allows time for any spores present in the alcohol or the new/re-used bottles to be destroyed.