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[DESCRIPTION]
<p>The <strong>Quick-DNA™ FFPE Kit</strong> provides a simple and reliable method for high yield/quality DNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples and sections. The unique chemistries of the product have been optimized for maximum recovery of non-crosslinked, ultra-pure DNA without RNA contamination. Simply digest deparaffinized tissues using the provided <strong>Proteinase K</strong>, heat, and then purify the DNA with the Fast-Spin columns in the kit. DNA &gt;50 bp or &gt;500 bp can be selectively isolated by altering the lysis buffer conditions as given in the protocol. PCR inhibitors are effectively removed during the isolation procedure, and eluted DNA is ideal for PCR, Next-Gen library prep, enzymatic manipulation, etc.</p>

[BEFORE_START]
<p><strong>Buffer Preparation</strong></p>
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<p>Add 260 μl <strong>Proteinase K Storage Buffer</strong> to reconstitute lyophilized <strong>Proteinase K</strong> at 20 mg/ml. Vortex to dissolve. Store at -20° C.</p>
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<p>Before starting, add 48 ml 100% ethanol (52 ml 95% ethanol) to the 12 ml <strong>Genomic DNA</strong></p>
<p><strong>Wash 2</strong> concentrate.</p>
<p> </p>
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<p>Resuspend lyophilized <strong>RNase A</strong> in 300 μl of ddH<sub>2</sub>0. Store at 4° C.</p>
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<p><em>Recommended:</em> Add beta-mercaptoethanol (user supplied) to the <strong>Genomic Lysis Buffer</strong> to a final dilution of 0.5%(v/v) i.e., 250 μl per 50 ml.</p>
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[GUIDELINES]
<p><strong>Product Contents</strong></p>
<p> </p>
<p><img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/gtvc2w6.png" width="450" data-src="https://s3.amazonaws.com/pr-journal/gtuc2w6.png" data-ofn="Screen Shot 2016-11-25 at 11.37.27 AM.png" /></p>
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<div class="layoutArea"><sup>1</sup> The Proteinase K is stable as shipped. Add 260 µl <strong>Proteinase K Storage Buffer</strong> to each <strong>Proteinase K</strong> tube prior to use. The final concentration of <strong>Proteinase K</strong> after the addition of <strong>Proteinase K</strong> storage Buffer is ~20 mg/ml. Store at -20° C.</div>
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<div class="layoutArea"><sup>2</sup> Recommended: Add beta-mercaptoethanol to 0.5(v/v) i.e., 250 µl per 50 ml or 500 µl per 100 ml.</div>
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<div class="layoutArea"><sup>3</sup> Before starting, add 48 ml 100% ethanol (52 ml 95% ethanol) to the 12 ml <strong>Genomic DNA Wash 2</strong> concentrate. </div>
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<div class="layoutArea"><sup>4 </sup>Re-suspend lyophilized RNase A in 300 µl of ddH<sub>2</sub>O. Store at 4° C.</div>
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<div class="layoutArea"><strong>Specifications</strong></div>
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<li><strong>Sample Size</strong> Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 cm<sup>2</sup> . It is recommended to use 1-2 sections if performing the protocol for the first time. Compatible with fresh/frozen tissue specimens.</li>
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<li>
<p><strong>DNA Recovery</strong>  High quality total DNA (A<sub>260/</sub>A<sub>280</sub> &gt;1.8) can be eluted into small volumes (i.e., ≥25 µl) allowing for highly concentrated samples. The maximum DNA binding capacity of the provided spin column is ~25 µg.</p>
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<li>
<p><strong>Processing Time</strong> As little as 4 hours when processing large amounts of tissue. For maximum yields of the highest quality DNA, it is recommended to process samples overnight.</p>
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<p><strong>Equipment/Reagents</strong> Microcentrifuge, thermomixer or heat block/bath capable of 55°C and 90°C, isopropanol, beta-mercaptoethanol (optional).</p>
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<p> </p>
<p><strong>Product Description</strong></p>
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<p>The <strong>Quick-DNA™ FFPE Kit</strong> provides a simple and reliable method for high yield/quality DNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples and sections. The unique chemistries of the product have been optimized for maximum recovery of non-crosslinked, ultra-pure DNA without RNA contamination. Simply digest deparaffinized tissues using the provided <strong>Proteinase K</strong>, heat, and then purify the DNA with the Fast-Spin columns in the kit. DNA &gt;50 bp or &gt;500 bp can be selectively isolated by altering the lysis buffer conditions as given in the protocol. PCR inhibitors are effectively removed during the isolation procedure, and eluted DNA is ideal for PCR, Next-Gen library prep, enzymatic manipulation, etc. Shown below is a schematic and performance overview of the procedure.</p>
<p> </p>
<p><img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/gtwc2w6.png" width="450" data-src="https://s3.amazonaws.com/pr-journal/gtwc2w6.png" data-ofn="Screen Shot 2016-11-25 at 11.45.40 AM.png" /></p>
<p> </p>
<p><strong>Buffer Preparation</strong></p>
<p> </p>
<div class="page" title="Page 4">
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<p>Add 260 µl <strong>Proteinase K Storage Buffer</strong> to reconstitute lyophilized <strong>Proteinase K</strong> at 20 mg/ml. Vortex to dissolve. Store at -20° C.</p>
<p> </p>
<div class="page" title="Page 4">
<div class="layoutArea">
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<p>Before starting, add 48 ml 100% ethanol (52 ml 95% ethanol) to the 12 ml <strong>Genomic DNA</strong></p>
<p><strong>Wash 2</strong> concentrate.</p>
<p> </p>
<div class="page" title="Page 4">
<div class="layoutArea">
<div class="column">
<p>Resuspend lyophilized <strong>RNase A</strong> in 300 µl of ddH<sub>2</sub>0. Store at 4° C.</p>
<p> </p>
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<p><em>Recommended:</em> Add beta-mercaptoethanol (user supplied) to the <strong>Genomic Lysis Buffer</strong> to a final dilution of 0.5%(v/v) i.e., 250 µl per 50 ml.</p>
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