TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>The <strong>Zyppy™ Plasmid Miniprep Kit</strong> features a <strong>Pellet-Free™</strong> modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated <strong>7X Lysis Buffer</strong> directly to your bacterial culture, neutralize, then purify using our Fast-Spin column technology (alternatively, the samples may be processed by the classical centrifugation method). Additionally, the innovative colored buffers included in the kit permit error-free visualization identification of complete bacterial cell lysis and neutralization.</p>

[BEFORE_START]
<p>The procedure is performed at room temperature.</p>
<p> </p>
<p><strong>Buffer Preparation:</strong></p>
<p> </p>
<div class="page" title="Page 4">
<div class="layoutArea">
<div class="column">
<ol>
<li>
<p>Add 24 ml of 100% ethanol (26 ml of 95% ethanol) to the 6 ml <strong>Zyppy™ Wash Buffer</strong> concentrate (D4036), 48 ml of 100% ethanol (52 ml of 95% ethanol) to the 12 ml <strong>Zyppy™ Wash Buffer</strong> concentrate (D4019), or 192 ml of 100% ethanol (208 ml of 95% ethanol) to the 48 ml <strong>Zyppy™ Wash Buffer</strong> concentrate (D4020 &amp; D4037) before use.</p>
</li>
<li>
<p>The <strong>7X Lysis Buffer</strong> may have precipitated during shipping. To completely resuspend the buffer, incubate the bottle at 30 – 37 °C for 30 minutes and mix by inversion. DO NOT MICROWAVE.</p>
</li>
</ol>
<p> </p>
<p> </p>
</div>
</div>
</div>

[GUIDELINES]
<p><strong>Product Contents</strong></p>
<p> </p>
<p><img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/gt4c2w6.png" width="450" data-src="https://s3.amazonaws.com/pr-journal/gt3c2w6.png" data-ofn="Screen Shot 2016-11-25 at 12.33.04 PM.png" /></p>
<div class="page" title="Page 2">
<div class="layoutArea">
<div class="column">
<p>Note ─ Integrity of kit components is guaranteed for up to one year from date of purchase. Reagents are routinely tested on a lot-to-lot basis to ensure they provide maximal performance and reliability.</p>
<p> </p>
<div class="page" title="Page 2">
<div class="layoutArea">
<div class="column">
<p><sup>1 </sup>The 7X Lysis Buffer may have precipitated during shipping. To completely resuspend the buffer, incubate the bottle at 30 – 37 °C for 30 minutes and mix by inversion. DO NOT MICROWAVE.</p>
<p> </p>
<div class="page" title="Page 2">
<div class="layoutArea">
<div class="column">
<p><sup>2 </sup>Neutralization Buffer contains RNase A at a concentration of 200 μg/ml.</p>
<p> </p>
<div class="page" title="Page 2">
<div class="layoutArea">
<div class="column">
<p><sup>3 </sup>Add 24 ml of 100% ethanol (26 ml of 95% ethanol) to the 6 ml Zyppy™ Wash Buffer concentrate (D4036), 48 ml of 100% ethanol (52 ml of 95% ethanol) to the 12 ml Zyppy™ Wash Buffer concentrate (D4019), or 192 ml of 100% ethanol (208 ml of 95% ethanol) to the 48 ml Zyppy™ Wash Buffer concentrate (D4020 &amp; D4037) before use.</p>
<p> </p>
<div class="page" title="Page 2">
<div class="layoutArea">
<div class="column">
<p><sup>4 </sup>Caution: 7X Lysis Buffer contains NaOH and Neutralization Buffer contains chaotropic reagents. Please use proper safety precautions with these reagents.</p>
<p> </p>
<p><strong>Product Description</strong></p>
<p> </p>
<div class="page" title="Page 3">
<div class="layoutArea">
<div class="column">
<p>The <strong>Zyppy™ Plasmid Miniprep Kit</strong> features a <strong>Pellet-Free™</strong> modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated <strong>7X Lysis Buffer</strong> directly to your bacterial culture, neutralize, then purify using our Fast-Spin column technology (alternatively, the samples may be processed by the classical centrifugation method). Additionally, the innovative colored buffers included in the kit permit error-free visualization identification of complete bacterial cell lysis and neutralization.</p>
<p> </p>
<p>Our <strong>Zyppy™ Plasmid Miniprep Kit</strong> is the fastest and simplest method available to efficiently separate plasmid DNA from E. coli. The plasmid DNA is of the highest quality, is endotoxin-free, and is well suited for use in bacterial transformation, restriction endonuclease digestion (below right), DNA ligation, PCR, transcription, sequencing (below), and other sensitive downstream applications. An overview of the purification procedure is shown to the right.</p>
<p> </p>
<p><img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/gt5c2w6.png" height="400" data-src="https://s3.amazonaws.com/pr-journal/gt5c2w6.png" data-ofn="Screen Shot 2016-11-25 at 12.42.42 PM.png" /> <img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/gt7c2w6.png" width="450" data-src="https://s3.amazonaws.com/pr-journal/gt7c2w6.png" data-ofn="Screen Shot 2016-11-25 at 12.44.46 PM.png" /></p>
<p><img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/gt8c2w6.png" width="200" data-src="https://s3.amazonaws.com/pr-journal/gt8c2w6.png" data-ofn="Screen Shot 2016-11-25 at 12.45.15 PM.png" /></p>
<p> </p>
<p>Specifications</p>
<p> </p>
<div class="page" title="Page 4">
<div class="layoutArea">
<div class="column">
<ul>
<li>
<p><strong>  DNA Purity</strong>: Plasmid DNA is well suited for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. Typical Abs<sub>260</sub>/<sub>280</sub> index is ≥ 1.8.</p>
</li>
<li>
<p><strong>Plasmid DNA Yield:</strong> Up to 25 μg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized.</p>
</li>
<li>
<p><strong>  Plasmid DNA Size</strong>: Up to 25 kb.</p>
</li>
<li>
<p><strong>  Recovery Volume</strong>: ≥ 30 μl.</p>
</li>
<li>
<p><strong>  Procedure</strong>: Performed at room temperature (15-30°C).</p>
</li>
</ul>
<p> </p>
<p><strong>Buffer Preparation:</strong></p>
<p> </p>
<div class="page" title="Page 4">
<div class="layoutArea">
<div class="column">
<ol>
<li>
<p>Add 24 ml of 100% ethanol (26 ml of 95% ethanol) to the 6 ml <strong>Zyppy™ Wash Buffer</strong> concentrate (D4036), 48 ml of 100% ethanol (52 ml of 95% ethanol) to the 12 ml <strong>Zyppy™ Wash Buffer</strong> concentrate (D4019), or 192 ml of 100% ethanol (208 ml of 95% ethanol) to the 48 ml <strong>Zyppy™ Wash Buffer</strong> concentrate (D4020 &amp; D4037) before use.</p>
</li>
<li>
<p>The <strong>7X Lysis Buffer</strong> may have precipitated during shipping. To completely resuspend the buffer, incubate the bottle at 30 – 37 °C for 30 minutes and mix by inversion. DO NOT MICROWAVE.</p>
</li>
</ol>
<p><strong>Troubleshooting:</strong></p>
<p>Please see the <a href="http://www.zymoresearch.com/downloads/dl/file/id/47/d4036i.pdf" target="_blank">product PDF</a>.</p>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.