eamag/protocols-with-steps huggingface dataset:
TITLE: Apple Cider Mimosa Recipe
AUTHORS: Diana Curcio

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">protocols.io Team Friendsgiving 2020</div><div class = "text-block">This protocol is a quick recipe for how to make an apple cider mimosa. </div></div>

[STEPS]
?. [Prep]
Ingredients:• Zeigler's Apple Cider 64 oz – also works with any apple cider brand • 1 bottle of champagne, La Marca Prosecco is what I use, but also works with your preferred champagne • Optional add ons – Marzetti Caramel Dip, Classic 13.05 oz
To prep, keep all ingredients refrigerated until chilled.
?. [Mixing]
IF YOU CHOOSE TO ADD CARAMEL, start by taking a 12 oz glass and dipping the top of the glass into the caramel so it will be on the rim. IF YOU CHOOSE TO MAKE THE DRINK WITHOUT CARAMEL, proceed to the below steps.
?. [Mixing]
Pour 1/2 cup of champagne and 1/2 cup of apple cider in a 12 oz glass.
Be sure not to overpour, as it could cause the drink to become too sweet
If you prefer less champagne, shift to pouring 1/4 cup of champagne and 3/4 cup of apple cider
?. [Mixing]
Mix/stir well
?. [Enjoy!]
Once you have mixed the ingredients listed above, your apple cider mimosa is ready!
TITLE: HuBMAP | GE/URMC/SCH Cell DIVE™ Modality Overview
AUTHORS: Gloria S Pryhuber, gail.deutsch  , Christine  Surrette, Lisa  Lowery, heidie_huyck  , Cory  Poole

[DESCRIPTION]
This is an overview of all protocols currently in use for the GE/URMC/SCH Cell DIVE collaboration for the Human BioMolecular Atlas Program (HuBMAP). It includes links to each of the individual protocols that make up this project workflow.

[STEPS]
1. Confirm donor acceptance criteria for inclusion.

URMC | Donor Acceptance Criteria for GE/URMC HuBMAP Inclusion
URMC | COVID+ Donor Acceptance Criteria for GE/URMC HuBMAP Inclusion
2. Prepare paraffin blocks and FFPE sections from tissue samples.

URMC | Whole Lung and Lobe Processing - Formalin Fixation and Gross Sectioning of Tissue Samples
URMC | Parrafin Embedding and Sectioning of FFPE Lung Tissue
3. Deparaffinize and rehydrate slides.

Cell DIVE™ Platform | Slide Clearing and Antigen Retrieval
4. Characterize antibodies (primary/secondary, direct conjugates, and zenon labelled antibodies) and determine any antigen effects from the Cell DIVE dye inactivation process.

Cell DIVE™ Platform | Antibody Characterization for Multiplexing 
Cell DIVE™ Platform | Antibody Staining & Imaging

5. Prepare direct conjugates for study.

Cell DIVE™ Platform | Antibody Purification Chemistry
Cell DIVE™ Platform | Ab Conjugation: Initial Conjugation & Scale up Conjugation
6. Perform Cell DIVE™ multiplexed data acquisition on the final cohort.
TITLE: Neural differentiation of AAVS1-TRE3G-NGN2 pluripotent stem cells
AUTHORS: Jiuchun Zhang, Harper JW

[DESCRIPTION]
This protocol is about neural differentiation of AAVS1-TRE3G-NGN2 pluripotent stem cells.

[BEFORE_START]
Prepare ND1 Medium and ND2 Medium:

ND1 Medium
DMEM/F12
N2 (100x)            1x
BDNF                   10 ng/ml
NT3                      10 ng/ml
NEAA (100X)      1x
Laminin               0.2 μg/ml
Doxycycline        2 μg/ml

ND2 Medium
Neurobasal medium
B27 (50x)                        1x
Glutamax (100x)            1x
BDNF                               10 ng/ml
NT3                                  10 ng/ml
Doxycycline                     2 μg/ml


 


[STEPS]
SECTION: Day 0:
1. Treat H9 AAVS1-TRE3G-NGN2 cells with Accutase and plate the dissociated cells in matrigel-coated 6-well plates (2x105 cells/well) in ND1 Medium supplemented with Y27632 (10 micromolar (µM)).
SECTION: Day 1:
2. Replace the medium with ND1 Medium.
SECTION: Day 2:
3. Replace the medium with ND2 Medium.
SECTION: Day 4:
4. Exchange 50% of the medium from each well.
SECTION: Day 6:
5. Treat the cells with Accutase and replate the dissociated cells in matrigel-coated 6-well plates (2-4x105 cells/well) in ND2 Medium.
SECTION: Day 8 and thereafter:
6. Exchange 50% of the medium from each well every other day.
TITLE: Imaging of Calcium Dynamics in Vasoactive Intestinal Peptide-expressing Neurons of Enteric Nervous System
AUTHORS: Joseph  Margiotta, Marthe   Howard

[DESCRIPTION]
The protocol was published on behalf of the investigators by the SPARC project team. 

The University of Toledo Health Science Campus animal care and use committee approved animal care, breeding procedures, and experimental protocols. Animals were housed in an Association of Laboratory animal Care-approved facility with a 12-hour light cycle with food (standard chow) and water ad libitum.

One male VIP-GCAmP reporter mouse, age 4 months, was used in this study. The mouse was purchased from the Jackson Laboratories, and all genotyping was performed using primers recommended by the Jackson Laboratories according to their protocols. 

[STEPS]
3. Remove the colon. 
5. Pinn the colon segment mucosal side down onto a Sylgard surface lining a glass coverslip attached to the bottom of a plastic imaging chamber containing artificial CSF perfused and bubbled with Carbogen (95% O2/5% CO2) to oxygenate and achieve pH 7.4.
 

1. On the day of the experiment prepare solutions of:

Artificial cerebrospinal fluid (CSF) containing:

117.9 mmol/L NaCl
4.7 mmol/L KCL
25 mmol/L NaHCO3
1.3 mmol/L NaH2PO4
1.2 mmol/L MgSO4•7H2O
2.5 mmol/L CaCl2
11.1 mmol/L D-glucose
2 mmol/L sodium butyrate
20 mmol/L sodium acetate

  2. Artificial CSF with test reagents, e.g., agonists.
6. Detect GCaMP-positive neurons by their basal Ca2+ fluorescence.

 

4. Open the excised proximal colon segment along the mesenteric border.
2. Euthanize the mouse.
8. At second 20 focally deliver an agonist by pressure microperfusion (10psi,10 sec; via Picospritzer II; Parker Instrumentation Corp, Barnstaple, UK) from blunt glass micropipettes (diameter, 5–10 mm) delivered within 50 µm of an adjacent myenteric ganglion.

 

7. Record spontaneous and evoked changes in GCaMP6f-mediated Ca2+ fluorescence intensity.
TITLE: A protocol for computerized quantitative analysis of nerve fibers, mast cells, enteric glial cells and the proximity of mast cells to the nerve fibers in 3D Images of human sigmoid mucosal biopsies
AUTHORS: Tao Li, Pu-Qing Yuan, Yvette Taché

[DESCRIPTION]
This protocol describes a step-by-step computational workflow that we developed by adapting Imaris 9.7-9.9 Surfaces Rendering Technology (https://imaris.oxinst.com/products/imaris-for-neuroscientists) to perform the quantitative analysis of nerve fibers, enteric glial and immune cells in 3D images of human sigmoid mucosal biopsies, as well as to assess the proximity of mast cells to the nerve fibers which cannot be easily portrayed and precisely measured with 2D images. The volumes of surface-masked nerve fibers and enteric glial cells, the numbers of spotted mast cells and the shortest distances of the centers of each individual spot to the surfaces of nerve fibers in 3D images were automatically computed and plotted correspondently with Imaris 9.7-9.9. This computerized protocol not only reduces the biases due to observer/examiner
judgment and overcomes limitations of 2D images, but also much faster than measuring manually and allows us to quantitate a larger number of samples, increasing statistical accuracy. The created parameters also provide efficient reference for us to apply the New AI Machine Learning Segmentation implemented into Imaris 10.1 recently for our quantitative analysis of large datasets. This protocol has been using in out study relevant to the underlying peripheral mechanisms of visceral pain in irritable bowel syndrome.

[STEPS]
1. 
2.
TITLE: Assessment of cognition as a predictor of prognosis in inpatients with brain damage: A Scoping Review Protocol
AUTHORS: Michihito  Mitsuyasu, Yuro Yamauchi, Daichi Ando, Takashi  Kitagawa

[DESCRIPTION]
Objective: 
The purpose of this study is to determine what cognitive assessments are used to predict or determine outcomes in inpatients with brain damage.  
Introduction:  
The prognosis of hospitalized stroke and brain injury patients is related not only to physical function but also to cognition. Global mental functions such as the Mini-Mental State Exam (MMSE), Montreal Cognitive Assessment (MoCA), and Functional Independence Measure (FIM) are commonly used to assess cognition in stroke patients. It is not clear whether these assessments are used because they have better predictive discriminating ability than other assessments.
Inclusion criteria: 
Patients with stroke, traumatic cerebral hemorrhage, or subarachnoid hemorrhage who are hospitalized are included in the study. Eligibility criteria will consist of studies that use cognitive assessments (global mental functions, higher brain function, memory, attention, neglect, apraxia, disorientation, executive function, multitasking, apathy) as exposure or covariates and examine the association between gait, falls, hospital discharge, activities of daily living, and quality of life. The study design will be observational, but case studies (case reports, case series), intervention studies, and systematic reviews will be excluded. In addition to peer-reviewed articles, conference abstracts will be included in the search. Countries and languages are not restricted. 
Methods: 
Databases (PubMed, Web of Science, Scopus, CINAHL, Igaku Chuo Zasshi) will be used for searching. NPO Japan Medical Abstracts Society operates Igaku Chuo Zasshi. Results will be tabulated by country, publication type, study design, type of analysis (univariate or multivariate), names of cognitive assessments and when to assess them, and type and timing of theoutcome. In addition, we will also add whether there are exclusion criteria due to cognitive impairment such as aphasia and whether studies are comparing the MMSE, MoCA, and FIM-Cog with other cognitive assessments.

[STEPS]
TITLE: CFE Expression and Lyophilization of β-Galactosidase (LacZ)
AUTHORS: Felipe Navarro Martínez, Anibal Arce Medina, Fernan Federici

[DESCRIPTION]
This protocol includes the expression and lyophilization of the β-Galactosidase enzyme (from the LacZ gene) in a cell-free system. We used it to prepare and conduct experiments for a biochemistry lab course. 

The cell-free extracts and buffers were prepared according to:
Guzman-Chavez, F., Arce, A., Adhikari, A., Vadhin, S., Pedroza-Garcia, J. A., Gandini, C., Ajioka, J. W., Molloy, J., Sanchez-Nieto, S., Varner, J. D., Federici, F., & Haseloff, J. (2022). Constructing Cell-Free Expression Systems for Low-Cost Access.ACS synthetic biology,11(3), 1114–1128. https://doi.org/10.1021/acssynbio.1c00342


The lyophilization steps for cell-free systems were adapted from:
Jung, J. K., Alam, K. K., Verosloff, M. S., Capdevila, D. A., Desmau, M., Clauer, P. R., ... & Lucks, J. B. (2020). Cell-free biosensors for rapid detection of water contaminants.Nature Biotechnology,38(12), 1451-1459.

[STEPS]
SECTION: CFE Expression and Lyophilization of β-Galactosidase
1. 
 

For the cell-free expression of β-Galactosidase, we prepare the following reaction according to the guidelines of this article:

 

 

SECTION: CFE Expression and Lyophilization of β-Galactosidase
2. Vortex the tubes. Incubate   at   in an orbital shaker.
 

 

SECTION: CFE Expression and Lyophilization of β-Galactosidase
3. Prepare the CFE reactions for lyophilization by diluting 200 µL of the reaction in PBS 1X to a final volume of 2500 µL (1/12.5 dilution).
SECTION: CFE Expression and Lyophilization of β-Galactosidase
4. Vortex and make 50 µL aliquots. Seal each tube with aluminum foil or parafilm.
 

SECTION: CFE Expression and Lyophilization of β-Galactosidase
5. Place the tubes at 80 °C for 20-30 min to allow slow freezing. 

SECTION: CFE Expression and Lyophilization of β-Galactosidase
7. Transfer the tubes to a freeze-dryer and lyophilize the tubes for at least 120 min.
 

SECTION: CFE Expression and Lyophilization of β-Galactosidase
8. Remove the tubes from the drying chamber, quickly remove the parafilm and close the tubes. 
 

SECTION: CFE Expression and Lyophilization of β-Galactosidase
9. Unless rehydrated immediately, place the tubes in a light protective bag with a silica gel desiccant, an oxygen absorber and a shot of argon using an argon canister to achieve an anoxic environment. Vacuum seal the bag using a vacuum sealer. 
SECTION: Rehydration of β-Galactosidase
11. To rehydrate the lyophilized enzyme (seen as a white pellet on the tube), spin down and add 1 mL of PBS 1X. Vortex for 40 s to favor the homogeneous resuspension of the protein inside the tube.
 


SECTION: CFE Expression and Lyophilization of β-Galactosidase
10. Store the bags protected from light at 4 °C or RT

SECTION: CFE Expression and Lyophilization of β-Galactosidase
6. Perform small punctures in the seal of each tube using a small 1mL syringe or a similar instrument.
TITLE: Bacteria Transformation
AUTHORS: Carolina Lopez

[DESCRIPTION]
General protocol for bacteria transformation

[STEPS]
SECTION: Bacteria Transformation:
1. 1. Pipette 2 µL of product to 50 µL of E. coli competent cells and pipette slowly up and down 5 times to mix.  For plasmids, use 2 µL  ul of 0.005 µg/µLplasmid solution. For ligation reactions, DNA concentrations will vary so use 2 µL ul of reaction. 
2. Incubate for 20 min on ice.
3. Heat shock bacteria in 42 °C waterbath for 1 min.
4. Incubate on Ice for 3 min.
5. Add 100 µL of SOC media and shake in warm room for 60 min.
6. Pipette 100 µL of bacteria onto LB-Antibiotic Plate and spread cells out with plate spreader to get individual colonies.
7. Incubate overnight in 37 °C warm room. 
8. Pick colonies for miniprep growth and sequencing. 
SECTION: Glycerol Bacteria Stocks:
2. 1. In screw top 2 mL tube. Mix 500 µL of Bacteria Solution (Either from Miniprep or Maxiprep before centrifugation) and 500 µL of 50% (v/v) Glycerol Solution.  
2. Store at -80 °C. 
3. To pull out bacteria, use bacteria loop to scrap some of still frozen glycerol stock and streak bacteria onto LB-Antibiotic plate. Then pick colonies for growth.
TITLE: Colloidal Coomassie Blue Stain: Recipe and Protocol
AUTHORS: Jeremy Kamil

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">I learned this Colloidal Coomassie Blue staining recipe and protocol when I was a postdoc at Harvard Medical School.. Rumor has it, a postdoc reverse engineered a “safe stain” from a leading commercial supplier. It’s a really great recipe: much more sensitive, faster, and easier to use than traditional methanol based coomassie staining protocols you see in Cold Spring Harbor books, like by Maniatis etc..  Saves a ton of money compared to buying this stuff from commercial vendors.  It is downstream compatible with mass spec and most other things you'd want to do with gel slices. </div></div>

[STEPS]
?. Dissolve 0.5 grams Coomassie Brilliant Blue G powder in 250 mL ethanol (200 proof), mix very well on stir bar until it seems fully or very nearly fully dissolved.
?. Filter the Coomassie Blue G in ethanol solution through 0.2 or 0.45 um membrane, like a bottle top vaccum filter, to produce a solution that is free of any insoluble Brilliant Blue particles (Do not skip this step!)
?. In a large graduated cylinder or other suitible container, 4.75 L of distilled (or pure) water, dissolve 105.1 grams of citric acid monohydrate (sufficient material to produce a 0.1 M citric acid monohydrate solution when brought to 5 L.
?. Dump the filtered 250 mL of ethanol containing 0.5 grams of dissolved Brilliant Blue G into the 4.75 L citric acid monohydrate solution, bringing it to 5L of 0.1 M citric acid monohydrate and 0.1% Brilliant Blue G.  Mix well.
?. To stain an SDS-PAGE protein gel using this staining reagent, gently transfer an SDS-PAGE gel into ~50-100 mL of distilled or deionized water and microwave for approximately 30-45 seconds, until the water is very warm but not boiling.  Then carefully dump off the water and replace with 50-70 mL of colloidal coomassie blue staining solution (the product of step 4).  You want just enough to fully cover the gel.  Microwave the gel + stain solution again for 30-45 seconds or until very warm.  Let the gel rock on a rocking platform for 3-10 minutes while checking occassionally how the bands are developing.  Once fully stained (takes usually no longer than 15 min) you can dump off the staining solution and replace with 100-150 mL of water.  Microwave the gel in water for 45 seconds to heat it, and rock on a rocking platform with a tiny crumpled piece of kim wipe or paper napkin to accelerate destaining process.  You can change out the water and keep replacing the kimwipe until the destaining is complete enough.  Or just leave it out on your bench overnight in water.. the will destain nicely.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
This protocol outlines the analysis used to plot contig coverage stats, as well as sequence count and length stats. We begin with visualizing contig length vs coverage. We then visualize the distributions of sequence counts per sample as a probability density plot (similar idea as a histogram), and then do the same for median sequence length. Based on the methods from the following publication:<br /><br />Hannigan, Geoffrey D., et al. "The Human Skin Double-Stranded DNA Virome: Topographical and Temporal Diversity, Genetic Enrichment, and Dynamic Associations with the Host Microbiome." <em>mBio</em> 6.5 (2015): e01578-15.<br /><br />

[BEFORE_START]
Supplemental informtion available at:<br /><br /><a href="https://figshare.com/articles/The_Human_Skin_dsDNA_Virome_Topographical_and_Temporal_Diversity_Genetic_Enrichment_and_Dynamic_Associations_with_the_Host_Microbiome/1281248" target="_blank">https://figshare.com/articles/The_Human_Skin_dsDNA_Virome_Topographical_and_Temporal_Diversity_Genetic_Enrichment_and_Dynamic_Associations_with_the_Host_Microbiome/1281248</a>

[GUIDELINES]
<span class="identifier">sessionInfo</span><span class="paren">(</span><span class="paren">)<br /><br />## R version 3.2.0 (2015-04-16) <br />## Platform: x86_64-apple-darwin13.4.0 (64-bit) <br />## Running under: OS X 10.10.4 (Yosemite) <br />## ## locale: <br />## [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 <br />## <br />## attached base packages: <br />## [1] stats graphics grDevices utils datasets methods base <br />## <br />## loaded via a namespace (and not attached): <br />## [1] magrittr_1.5    formatR_1.2    tools_3.2.0    htmltools_0.2.6 <br />## [5] yaml_2.1.13    stringi_0.4-1    rmarkdown_0.7    knitr_1.10.5 <br />## [9] stringr_1.0.0    digest_0.6.8    evaluate_0.7</span>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
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?. 
?. 
?. 
?. 
?.
TITLE: Activation of Human T cells with Phytohaemagglutinin (PHA) 
AUTHORS: Zaki Molvi

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Activating T cells with Phytohaemagglutinin (PHA) is a cheap and easy way to initiate a culture of rapidly growing T cells from human samples. PHA-activated T cells, or PHA blasts, can be used for downstream applications such as transduction (e.g. of a CAR or TCR) or as target cells for CTLs since they express sufficiently high levels of HLA class I. An especially useful application is for QCing a batch of frozen PBMCs -- if PHA blasts do not grow in 5 days from a given batch, then it is unlikely that such cells are in adequate condition for more sensitive in vitro applications. I describe here how I like to make them.</div></div>

[STEPS]
?. Resuspend PBMCs at 2e6 cells/mL in complete medium (e.g. IMDM + 2mM L-Glutamine + 10% FBS). Add PHA to a final concentration of 5-10ug/mL (PHA-L or PHA-P both work in my experience). Incubate for 5 days in an appropriate vessel for your culture size (both flasks and plates will work, even if the latter is not TC coated).
?. Incubate for 5 days at 37C.
?. On day 5-6, growing clusters and acidified media should be visible by naked eye. Count cell density. If density is over 1e6 cells /mL, adjust to 1e6 cell /mL with media. If density is at or below 1e6 cells/mL, spin down half of the culture and resuspend in the same volume with fresh media. Add IL2 to a final concentration of 50U/mL. Incubate for 2-3 days.
?. After 2-3 days, and every 2-3 days thereafter, add IL2 to a final concentration of 50U/mL (always assume all of the IL2 from previous feedings is completely consumed). If cell numbers drop, increase IL2 dose by 50U/mL. Use this increased dose for subsequent feedings. Around day 10-14, restimulate cells with 5-10ug/mL PHA if you will not be freezing them.
?. By day 10, I usually observe that CD4 T cells preferentially grow compared to CD8 T cells (CD4:CD8 of > 70:30), and such CD4 T cells express moderate to high levels of HLA class I, making them ideal for assay of class I-restricted effector cells. Phenotype may change depending on inclusion of other cytokines in addition to IL2, such as IL15 or IL7. Enjoy your PHA blasts!
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
The Gibson Assembly <sup>®</sup> HiFi 1 Step method utilizes a blend of enzymes to assemble the DNA quickly and efficiently. The use of a High-Fidelity DNA polymerase enzyme allowsfor error free cloning resulting in a seamless construct ready for multiple downstream applications. The HiFi 1 Step method allows Gibson Assembly of up to 5 fragments using a one tube, one-step, isothermal reaction. Multiple DNA fragments can be joined with greater than 90% cloning efficiency. Large constructs up to 100kb in length can be efficiently assembled in multi stage reactions.

[STEPS]
?. 
?. 
?. 
?.
TITLE: Nielsen and Ford (2024) - Reduced striatal M4-cholinergic signaling following dopamine loss differentially contributes to parkinsonian and L-DOPA-induced dyskinetic behaviors
AUTHORS: Beatriz E Nielsen, Christopher P Ford

[DESCRIPTION]
This collection contains protocols detailing methods used in Nielsen and Ford (2024)-Reduced striatal M4-cholinergic signaling following dopamine loss differentially contributes to parkinsonian and L-DOPA-induced dyskinetic behaviors.



[STEPS]
TITLE: Collection and shipment of specimen for single-cell RNA sequencing (scRNA-Seq)
AUTHORS: Laura Robinson, Susan Sheehan, Gaven Garland, Ron Korstanje

[DESCRIPTION]
These samples are part of the JAX-Sen project in the SenNet Consortium. Here we provide details on specimen collection and shipment to the Robson laboratory at The Jackson Laboratory for Genomic Medicine (JAX-GM) in Farmington, CT for its processing for single-cell RNA-sequencing (scRNA-seq).

[STEPS]
SECTION: Reagents and Materials:
1. 2mL Eppendorf tubes or 5 ml Eppendorf tubes
MACS tissue storage Solution (#130-100-008, MACS Miltenyi Biotech), 
cold storage (4 degrees Celsius)
Tweezers (clean, sterile)
SECTION: Quality Key Points:
2. The tissue specimen should be kept at 4 degrees Celsius and RNAse-free from its excision until it is processed at JAX. 
It is crucial to not store the tissue specimen at RT to avoid any cell death, tissue and/or RNA degradation. 
SECTION: Procedure:
3. Timeline: The daily shipping deadline at Jax BH is 12:00 noon. So, harvest the tissues before and closer to noon.
SECTION: Procedure:
4. Collection/Harvest:
SECTION: Procedure:
4.1. Prepare sterile tubes with cold MACS tissue storage solution (the volume will depend on the size of the specimen, but should cover abundantly the tissue and fill up the tube)
SECTION: Procedure:
4.2. Animal was euthanized via cervical dislocation. 
SECTION: Procedure:
4.3. Animal was pinned to a necropsy tray which remained on wet ice throughout the harvest. 
SECTION: Procedure:
4.4. Animal was perfused with 20ml cold PBS.
SECTION: Procedure:
4.5. Heart, pancreas, and kidney was collected and the specimen was transferred immediately into cold MACS tissue storage solution in tubes, on ice.
SECTION: Procedure:
4.6. Individually parafilm the Eppendorf tube lids to their tubes before shipping.
SECTION: Procedure:
4.7. Keep at 4 degrees Celsius thereafter, until shipping.
SECTION: Procedure:
5. Shipping:
SECTION: Procedure:
5.1. Place sample tubes in a plastic box (cardboard boxes insulate the samples from the cold ice) or in double Ziplock bags after checking that they are completely sealed.
SECTION: Procedure:
5.2. Ship the sample box on wet ice.
SECTION: Procedure:
5.3. Ship O/N on ice/ice packs, to:
The Jackson Laboratory for Genomic Medicine,
Farmington, CT, 06032