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[DESCRIPTION]
<p>Rapid, Sensitive and Reversible Protein Stain For PVDF and Nitrocellulose Membranes.</p>
<p> </p>
<p>(These instructions are for a single 8 x 8 cm membrane.  Increase the reagent volumes with larger membranes.)</p>

[GUIDELINES]
<p><strong>INTRODUCTION</strong></p>
<p>Swift™ Membrane Stain is a unique, proprietary (patents pending), reversible, ready‐to‐ use membrane stain for proteins on nitrocellulose or PVDF membranes.  Swift™ Membrane Stain stains proteins faster and with 500X more sensitivity than the routinely used Ponceau‐S stain and other commercially available stains.  The lower detection limit of Swift™ Membrane Stain is ~0.5ng protein (BSA)/band.</p>
<p> </p>
<p>Swift™ Membrane Stain only stains proteins resulting in a clear background and no requirement for additional steps to remove background.  The stronger staining allows for easier image capture due to the strong blue stain on a clear, white background.  </p>
<p> </p>
<p>Swift Membrane Stain™ can be complete removed from the membrane in &lt;1 minute without affecting the biological or immunological properties of the immobilized proteins. This offers an advantage over Coomassie based stains as these are irreversible and can interfere with Western blotting.</p>
<p> </p>
<p><strong>*</strong>These instructions are for a single 8 x 8 cm membrane.  Increase the reagent volumes with larger membranes.</p>
<p> </p>
<p><strong>ITEM SUPPLIED</strong></p>
<table style="width: 421px; height: 138px;">
<tbody>
<tr>
<td style="width: 134.688px;"><strong>Cat. #</strong></td>
<td style="width: 133.576px;"><strong>786‐677</strong></td>
<td style="width: 132.465px;"><strong>786‐677S</strong></td>
</tr>
<tr>
<td style="width: 134.688px;">Swift™ Membrane Stain</td>
<td style="width: 133.576px;">250ml</td>
<td style="width: 132.465px;">25ml</td>
</tr>
<tr>
<td style="width: 134.688px;">Swift™ Destain [5X]</td>
<td style="width: 133.576px;">200ml</td>
<td style="width: 132.465px;">20ml </td>
</tr>
</tbody>
</table>
<p>The kit components are sufficient for 20 blots (786‐677) or 2 blots (786‐677S) of 8 x 8cm<br />size.</p>
<p><br /><strong>STORAGE &amp; STABILITY</strong><br />The kit is shipped at ambient temperature. Upon arrival, store reagents at room<br />temperature, storing reagents at 4°C will severely affect the performance of the stain.  <br />The kit components are stable for 12 months, when stored and handled properly.   </p>
<p> </p>
<p><strong>ITEMS NEEDED BUT NOT SUPPLIED</strong><br />• Staining trays (slightly larger than membrane)  <br />• Orbital shaker</p>
<p><br /><strong>PREPARATION BEFORE USE</strong><br />1. <em><strong>1X Swift™ Destain:</strong></em> Add 10ml Swift™ Destain [5X] to 40ml deionized water for each<br />blot and store at room temperature until required.</p>
<p> </p>
<p><strong>BACKGROUND STAINING</strong></p>
<p>Background staining depends on the types of membrane used for protein transfer. Nitrocellulose gives the clearest and most brilliant white background. Some PVDF membranes may give higher background. Background staining can be removed by shaking the membrane 5‐10 minutes in cold water.</p>

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