eamag/protocols-with-steps huggingface dataset:
TITLE: The efficacy of anti-stigma initiatives in reducing dementia stigma in young people: A systematic review protocol
AUTHORS: Mahfuza Ali, Esra Hassan, Nicolas Farina

[DESCRIPTION]
The aim of this study is to identify and describe the available published research on anti-stigma initiatives aimed at young people that seek to reduce dementia stigma. Stigma towards those  diagnosed with dementia is a recognised problem. The World Health Organisation (WHO) have devised a Global action plan that aims to improve the lives of people with dementia, so  they can live with dignity and respect. Integrated into the plan is a focus on dementia  awareness and friendliness which aims to reduce stigma using planned programmes that target a range of communities within the general public, including school students. Initiatives to reduce stigma have been described in previous papers however, this has  previously not been limited to adolescents. This is despite the WHO targeting namely  school students amongst the communities who would benefit from dementia awareness  and friendliness programmes. Therefore, this systematic review will focus on adolescents aged 10-18 years old, to understand whether such interventions are effective in reducing  dementia stigma.

[STEPS]
TITLE: End-preparation of amplicon pools
AUTHORS: Josh Quick

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This is a subprotocol for generating a library from a single amplicon pool</div></div>

[STEPS]
?. Set up the following reaction for each sample:Component                                          VolumeDNA amplicons (5ng/ul)                    Nuclease-free water                           Ultra II End Prep Reaction Buffer     Ultra II End Prep Enzyme Mix           Total
10 µl
2.5 µl
1.75 µl
0.75 µl
15 µl
?. Incubate at room temperature for  Incubate at  for Incubate on ice for
65 °C
TITLE: Look for Cell Invasion Experiment? How About Following Information?
AUTHORS: 181830691 

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">The cell transwell is put into the culture plate, the chamber was called the upper chamber, the culture plate was called the lower chamber, the upper chamber was filled with the upper culture medium, the lower chamber was filled with the lower culture medium, and the upper and lower culture medium were separated by polycarbonate membrane.The cells is planted in the upper chamber. Due to the permeability of the polycarbonate membrane, the components in the lower culture medium could affect the cells in the upper chamber, it is convenientto study the growth and movement of cells according to the effects conducted by the components in the lower culture medium.</div></div>

[STEPS]
TITLE: Illumina GAII Library Construction and Sequencing for RNA Seq
AUTHORS: Eric J. Carpenter, Naim Matasci, Shuangxiu Wu, Jing Sun, Jun Yu, Fabio Rocha Jimenez Vieira, Chris Bowler, Richard G. Dorrell, Matt Gitzendanner, Ling Li, Wensi Du, Kristian Ullrich, Michael S. Barker, James H. Leebens-Mack, Gane Ka-Shu Wong

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Illumina GAII Library Construction and Sequencing for RNA Seq</div></div>

[STEPS]
?. Isolate polyA RNA from   of total RNA treated by using .It is best to use up to 50 µg as the use of a lower mass (typically 20 µg) has been insufficient for successful library construction. This can be assessed by running final PCR products on an agarose gel; the library construction is considered to have failed when there was no visible band. It is possible to use less than 20 µg of total RNA when isolation of an important sample yielded low RNA mass but library construction was successful.
20 µg
?. Purified polyA RNA is fragmented in a at for  to 200–300 nt fragment sizes.
70 °C
?. The first cDNA strand is then synthesized with random hexamer primers using the .
?. The second-strand synthesis is performed by incubation with  and  .
?. Short double-stranded cDNA fragments are then purified using one of two methods.
?. Our standard procedure was to use the , whereas for samples with low RNA mass it is better to use .
?. Both methods are then followed by end-repair with Klenow polymerase,  .
?. A single 3′ adenosine (A base) was added to the double-stranded cDNA using and  .
?. The Illumina PE Adapter oligo mix is ligated onto the A base on repaired double-stranded cDNA ends and DNA fragments of a selected size are then gel-purified to make sure the insert size is 200 bp (±10% deviation).
?. Thereafter, libraries were amplified by 15 cycles of PCR with and “indexed” paired-end PCR primers; the prepared libraries were 322 bp long.
?. The amplified libraries were denatured with sodium hydroxide and diluted to  in hybridization buffer for loading into a GAII lane.
?. Read length on the GAIIx platform are typically adjusted to 73–75 bp (mean=74 bp), but can be read at 100 bp..
?. Samples are sequenced with paired-end reads, and average run times are about 5 days.
TITLE: Binding properties of Staphylococcal protein A (SpA) to purified immunoglobulins tested by double immunodiffusion (Ouchterlony) technique.
AUTHORS: Angel  Justiz-Vaillant

[STEPS]
?. The binding of SpA to purified IgG are investigated by double immunodiffusion.
?. Briefly, 1% agarose gels are prepared and wells cut into the gel using a template.
?. Initially, aliquots of 25 µl each of SpA diluted 1:5 are applied to the centre well.
?. The peripheral wells are filled with 25 µl each of purified IgG (1 µg/µl) in PBS pH 7.4.
?. The gels are incubated at RT for 48−72 hours.
?. After that the gels are examined for precipitin lines.
?. Human IgG are included as positive control and turtle serum as a negative control.
?. The positive results are taken as the presence of precipitin line/s and negative results, the absence of precipitin lines.
TITLE: Reverse transcription using SuperSript IV
AUTHORS: Amin Mahpour

[STEPS]
?. Mix the following reagents from the kit. Please scale up if more RT reaction is desired.  AB1ReagentAmount (uL)2Primer (Random or dT)0.53dNTP (10mM)14RNA11
AB1ReagentAmount (uL)2Primer (Random or dT)0.53dNTP (10mM)14RNA11
?. Incubate the mixture at   for . Then, incubate samples on ice for few minutes.
72 °C
This step allows denaturation of RNA and proper priming for the downstream cDNA synthesis.
?. Mix the following reagents from the kit. Please scale up if more RT reaction is desired.  AB1ReagentAmount (uL)2RT Buffer (5x)43DTT(10mM)14RNase Inhibitor1Add the 6uL to the 12.5uL mix from Step 3.
AB1ReagentAmount (uL)2RT Buffer (5x)43DTT(10mM)14RNase Inhibitor1
?. Incubate the samples at  for . Then, add 1.5uL SuperScript RT IV enzyme to the reaction and mix well.
37 °C
?. Incubate the samples using the following incubation settings:  AB1Temp (C)Time (minutes)2255345404551057510
AB1Temp (C)Time (minutes)2255345404551057510
?. Add 1uL RNase H to the cDNA samples and incubate at  for .
37 °C
?. Dilute the cDNA samples using Nuclease free water.
TITLE: Mixotrophic batch growth of Spirulina platensis
AUTHORS: Bruna Emerenciano, Maria Izabel Pereira Batista, Adriano H N Rangel

[STEPS]
?. 1. Prepare the batch culture in 6 L plastic bottles.
?. Introduce 10 % of inoculum into 3,6 L of Zarrouk medium as control cultivationTo mixotrophic cultivation introduce 10 % of inoculum into 4L of Zarrouk medium supplemented with 2.5%, 5.0% and 10% clarified buffalo mozzarella cheese whey. The initial S. platensis concentration should range from 0.2 to 0.3 g/L.
?. Then the culture should grown under the same light intensity and light regime as the inoculum (irradiance of 238 μmol m-2 s-1 under a photoperiod (12 h light/dark).
?. Keep the cultures under temperature of 25 °C and constant sterile aeration promoted by pumps (JAD Air Pump S-510) at a specific flow rate of 0.5 vvm (volume of air per volume of medium per minute).
?. All assays should performed in triplicate.
?. When the cultures reach the stationary phase, the biomasses should be collected by filtration on 20 μm screens and washed in distilled water to remove salt remnants, then the biomass will be lyophilized in a lyophilizer (LJJ02 - JJ Scientific) and frozen in a freezer at -20°C until beginning the chemical characterization.
TITLE: Streaking bacteria on an agar plate from a glycerol stock
AUTHORS: Cristian Riccio

[STEPS]
?. Transfer the glycerol stock onto dry ice next to a flame.
?. Rub the loop in the glycerol stock.
?. Streak the bacteri in zigzag over half the agar plate.
?. Sterilise the loop in the flame.
?. Sterilise the loop.
?. Zigzag over half the plate which overlaps with the first half by a quarter of a plate.
?. Sterilise the loop.
?. Zigzag over half the plate which overlaps with the second half by a quarter of a plate.
?. Incube overnight at the temperature which is optimal for bacterial growth, 37 C for E. coli.
?. The next day, you should have single colonies visible on a quarter of the plate or half the plate.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
This collection is part of the VERVE holiday drive to collect exotic off-the-shelf laboratory recipes. More details <a href="https://www.protocols.io/g/verve-net/news/call-for-recipes" target="_blank">here</a>.<br /><a href="https://twitter.com/whiskeyboy/status/677276040734089216" target="_blank"><br />From Larry Kane</a>: <em>Back when I was a tech before grad school, made my own instant coffee by lyophilizing coffee (and drank it)<br /><img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/byjcz36.jpg" alt="" data-src="https://s3.amazonaws.com/pr-journal/byicz36.jpg" data-ofn="11358520094_6f973d3059_o.jpg" /><br /><br /></em>(Image: <a href="https://www.flickr.com/photos/16210667@N02/11358520094/in/photolist-iiHoim-6iNxRA-5pQ1SW-eivx5S-dJEWe8-krbmsK-NertZ-74bVtD-66FUPR-81XjMw-76XdL2-HoPe6-j7bctV-7Mmoww-5RjmBr-5QC3vr-5Qqhrw-81XjNu-7i5GJs-4XGvog-7mDfYY-5S6Vp9-6uHJh5-Neo1o-5QWFFL-9jX18z-SUo5Z-7kNphi-8Enmy5-5R7jJ4-7MmiFw-SUkTi-STnD7-STpKE-5P8wRp-5PvWTf-bnTrvC-9k88fT-6Nd8JG-5wMeM8-4sGZeN-5wMdAv-4sH1Fm-7MhkxZ-abepdU-B8Q9Ma-eeabPW-siocBG-668ptS-5Kxnfh" target="_blank">Instant ! (Explored)</a>, Craig Sunter, CC BY-ND)

[STEPS]
?. 
?. 
?. 
?. 
?.
TITLE: Continuous positive airway pressure to reduce the risk of early peripheral oxygen desaturation following apnoea onset in children: Randomised double-blind controlled trial
AUTHORS: Jayme Marques

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>It was a randomised double-blind parallel controlled clinical trial, in a tertiary teaching hospital with 68 children, aging 2-6 years, ASA I-II, who presented for surgery under general anaesthesia, divided into two groups of 34 participants (CPAP and controls). The intervention was CPAP-ventilation and passive CPAP-oxygenation using the anaesthesia workstation. The main outcome measure was the occurrence of a SpO</span><span style = "vertical-align:sub;">2</span><span> of 95% during a follow-up period of 300 seconds from the beginning of the apnoea. We alternatively registered the time from the onset of apnoea until a SpO</span><span style = "vertical-align:sub;">2</span><span> of 95% (limited to 300 seconds) – with SpO</span><span style = "vertical-align:sub;">2</span><span> recorded each 30 seconds - (T1) and time from a SpO</span><span style = "vertical-align:sub;">2</span><span> of 95% to previous baseline levels aided by positive pressure ventilation (T2).</span><a href="#" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;"> </span></a><span> </span></div><br/></div>

[STEPS]
?. [Continuous positive airway pressure to reduce the risk of early peripheral oxygen desaturation following apnoea onset in children: Randomised double-blind controlled trial]
Continuous positive airway pressure to reduce the risk of early peripheral oxygen desaturation following apnoea onset in children: Randomised double-blind controlled trial
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TITLE: DNA Extraction from Yeast
AUTHORS: Brian    Teague

[DESCRIPTION]
This is a "quick and dirty" way to get some genomic DNA out of yeast cells. Not pure enough for many things, but should be fine for a PCR template.

This protocol is adapted from 
 
who in turn adapted it from
 



[GUIDELINES]
The centrifugation steps all specify 21000 x g . If your microcentrifuge doesn't go this high, spin at the fastest speed available.


[STEPS]
1. Make 100 µL of yeast lysis solution by mixing the following in a 1.7 ml microcentrifuge tube:
70 µL H2O
20 µL   solution, 1 Molarity (M)
10 µL   solution, 10 Mass / % volume 





2. Choose a yeast colony to analyze and circle it on the bottom of the petri dish. A large one is best.
3. Using a micropipette tip, scrape some of the colony off and resuspend it in the lysis solution. Vortex vigorously until the colony is mixed completely into the lysis solution.
 

4. Incubate at 70 °C for 5 min

5. Add 300 µL of  and vortex briefly. Centrifuge 21000 x g, 3 min  .
 



6. Using a P-1000 micropipettor, carefully aspirate the supernatant and discard as biological waste. Do not disturb the pellet.
7. Add500 µL of 70 % (v/v)   to the microcentrifuge tube. Centrifuge 21000 x g, 3 min. 
 


8. Using a P-1000 micropipettor, carefully aspirate the supernatant and discard as biological waste. Do not disturb the pellet. Try to get as much of the ethanol off as you can.
9. Let the pellet dry by leaving it in a 42 °C dry bath for 15 min. Leave the cap open.
 


10. Add 100 µL of   and vortex to resuspend the pellet.

11. Centrifuge 21000 x g, 30 s to collect the cellular debris at the bottom. The DNA remains suspended in the supernatant.

12. Label the tube and store at -20 °C , or proceed directly to PCR.

13. If you need to use this sample again:
Thaw the sample completely.
Vortex briefly to resuspend everything.
Centrifuge 21000 x g, 30 s to (re)collect the cellular debris at the bottom of the tube.
TITLE: Purification of FUNDC1-GFP
AUTHORS: Elias Adriaenssens

[DESCRIPTION]
This protocol details the purification of FUNDC1-GFP.

[STEPS]
SECTION: Purification - FUNDC1-GFP
1. To purify GFP-tagged 

FUNDC1-GFP (available from Addgene) or FUNDC1(Y18A/L21A)-GFP (ΔLIR) (available from Addgene), 

fuse the cytosol-exposed domain of FUNDC1 (1-50aa) to a C-terminal GFP-tag through cloning into a pET-DUET1 vector (available on Addgene). 
SECTION: Purification - FUNDC1-GFP
2. Introduce the point mutants by in vitro mutagenesis to generate 

FUNDC1 Y18A/L21A (ΔLIR)(available on Addgene). 
SECTION: Purification - FUNDC1-GFP
4. Once the cells reaches an OD600 of 0.8, induce protein expression with 100 micromolar (µM) isopropyl β-D-1-thiogalactopyranoside (IPTG) for 960 min at 18 °C. 
SECTION: Purification - FUNDC1-GFP
5. Collect the cells by centrifugation and resuspend in lysis buffer. 

Lysis buffer:

 
 
SECTION: Purification - FUNDC1-GFP
6. Sonicate the cell lysates twice for 30 s and clear by centrifugation at 18000 rpm, 45 min, 4 °C in a SORVAL RC6+ centrifuge with an F21S-8x50Y rotor (Thermo Scientific). 
SECTION: Purification - FUNDC1-GFP
7. Collect the supernatant and incubate with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare) for 120 min at 4 °Cwith gentle shaking to bind FUNDC1-GFP. 
SECTION: Purification - FUNDC1-GFP
6.1. Sonicate the cell lysates for 30 s (1/2).
SECTION: Purification - FUNDC1-GFP
6.2. Sonicate the cell lysates for 30 s (2/2).
SECTION: Purification - FUNDC1-GFP
8. Centrifuge the samples to pellet the beads and remove the unbound lysate. 
SECTION: Purification - FUNDC1-GFP
9. Wash the beads twice with wash buffer, once with high salt wash buffer, and two more times with wash buffer. 

Wash buffer:
 
 
 
High salt buffer:
 
 
 
SECTION: Purification - FUNDC1-GFP
10. Cleave off the GST-tag   by eluting the GFP-tagged cargo receptor from the GSH beads by the addition of TEV protease in wash buffer at 4 °C
SECTION: Purification - FUNDC1-GFP
11. To collect the supernatant, collect the beads by centrifugation. 
SECTION: Purification - FUNDC1-GFP
12. Wash the beads twice with 4 mL of wash buffer, and collect the supernatant. 
SECTION: Purification - FUNDC1-GFP
13. Pool the supernatant fractions, filtered through a 0.45 µm syringe filter, concentrated with 10 kDa cut-off Amicon filter (Merck Millipore), and load onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva). 
SECTION: Purification - FUNDC1-GFP
14. Elute the proteins with SEC buffer. 

 SEC buffer: 

 
 
SECTION: Purification - FUNDC1-GFP
15. Analyze fractions by SDS-PAGE and Coomassie staining. Pool the fractions containing purified FUNDC1-GFP. 
SECTION: Purification - FUNDC1-GFP
16. After concentrating the purified protein, aliquote the  protein and snap-frozen in liquid nitrogen. 

 

SECTION: Purification - FUNDC1-GFP
3. After the transformation of the pET-DUET1 vector encoding FUNDC1-GFP wild-type or mutants in E. coli Rosetta pLysS cells (Novagen Cat# 70956-4), grow the cells in 2x Tryptone Yeast extract (TY) medium at 37 °C until an OD600  of 0.4 and then continue at 18 °C.
TITLE: Immunofluorescence Staining of Brain Slices
AUTHORS: tomdeerinck

[DESCRIPTION]
Primary antibody labeling to a specific protein using a fluorescence secondary anitbody to label it. 

[STEPS]
1. Rinse tissues with ice cold 1x PBS
      3x @ 10 min 
2. Make up blocking buffer
      1x PBS, containing
      3% normal donkey serum
      1% bovine serum albumin
      1% Gelatin from cold water fish skin (G7765 Sigma-Aldrich)
      0.1% Triton X-100
3. Block tissues in blocking buffer 1hr on ice
4. Rinse tissues with ice cold working buffer
     1x PBS, containing
     10% Blocking Buffer
     0.1~0.5% Triton X-100
5. Make double- or triple- 1stAbs cocktail in Working Buffer
6. Incubate with 1stAbs solution 24 to 72 hrs at 4°C
7. Rinse with ice-cold working buffer
     6x @ 5 min
8. Dilute double- or triple- 2nd Abs cocktail in ice-cold working buffer
9. Incubate with 2nd Abs solution 2hr at 4°C
10. Rinse with 1x PBS    
       3x @ 5 min
11. Incubate tissues with DAPI or Hoechst 33342 solution for 5 minutes
12. Rinse with 1x PBS    
       3x @ 5 min
13. Mount cover slip using mounting medium
TITLE: iPSC Restriction Digest: For Screening Edited Clones
AUTHORS: Celeste Karch, Rita Martinez, Jacob Marsh

[STEPS]
?. Prepare the following reagents in a strip cap tube with the previously identified enzyme.  ABC1Restriction Digest Protocol2ReagentVolume# of rxns3Buffer (Cut Smart/ NEB)2 µl4DNA from PCR17.75 µl5Enzyme (specific)0.25 µl6Total20 µl
ABC1Restriction Digest Protocol2ReagentVolume# of rxns3Buffer (Cut Smart/ NEB)2 µl4DNA from PCR17.75 µl5Enzyme (specific)0.25 µl6Total20 µl
?. Incubate reaction at temperature ideal for the enzyme being used (e.g. 37°C , 42°C, 25°C, etc.)
?. Incubate at appropriate temperature for 2-3 hours.
?. Run the enzyme reaction on a gel to visualize product.
?. To make the gel, combine an appropriate amount of Agarose, TBE and Ethidium Bromide using the following guidelines. a. The 2% gel will be cast in one of the following ways:  ABC115x15 cast15x25 cast2Agarose1.5 g3.0 g3TBE75 mL150 mL4Ethidium Bromide3.75 uL7.5 uLb. Combine Agarose and TBE in an appropriately sized flask and microwave until Agarose is completely dissolved. Swirl intermittently during heating.c. Once completely dissolved add appropriate amount of Ethidium Bromide to flask and swirl until dispersed evenly.
ABC115x15 cast15x25 cast2Agarose1.5 g3.0 g3TBE75 mL150 mL4Ethidium Bromide3.75 uL7.5 uL
?. Pour gel from flask into casting tray (be sure to add appropriate amount of combs to casting tray).
?. Let sit for 30-40 minutes, or until firm.
?. Place gel cast into the gel rig apparatus.
?. Load samples.
?. Load 50 bp ladder.
?. Place lid on gel rig apparatus.
?. Run gel at 150 volts for  (checking at  to ensure samples have not run too far or off the gel).
?. Turn off gel rig apparatus and remove cast.
?. Blot off excess TBE from cast.
?. Analyze gel images and select potentially edited clones based on banding patterns.
TITLE: TIMSTOFPro Crosslinking Optimized for PhoX
AUTHORS: Johannes Hevler

[DESCRIPTION]
crosslinking.m:

Method to run cross-linking samples on TimsTof pro instrument. Method and energies are specifically
optimized for PhoX cross-linker reagent (by Richard Scheltema and Markus Lubeck (Brucker))

Standard_DDA_PASEF_1.1sec_cycletime_2segm_1st_15min_nospectra.m:

Method for classical bottom-up (proteomics) experiments. Optimized for LC systems that are operated 
without a trap and are euqipped with a 5 µL sample loop (flowrate 0.400 µL/min), as the Method is segmented: The
first 15 min of the method no spectra are saved. To further reduce the file size the noise filtering for Tims is turned on. (by Richard Scheltema and Markus Lubeck (Brucker))

[STEPS]
1.
TITLE: Single-cell sequencing and analysis
AUTHORS: Daniele  Neri, Lori  Zeltser

[DESCRIPTION]
This protocol describes single-cell sequencing and analysis.

[STEPS]
SECTION: Sequencing
1. We performed plate-based single-cell sequencing as described in Snyder et al., 2019 (https://doi.org/10.1126/sciimmunol.aav5581)
SECTION: Sequencing
2. Pooled 3’-end sequencing libraries were sequenced on an Illumina NextSeq 500/550 platform
SECTION: Alignment and count matrix generation
3. The reads were aligned to mouse genome, GRCm38, gencode version M13 (Ensembl 88) using STAR v2.5.3a
SECTION: Alignment and count matrix generation
4. The aligned reads were assigned to genes using featureCounts v1.5.3.
SECTION: Alignment and count matrix generation
5. bam files were sorted and indexed using Samtools v1.4.1, duplicated reads were removed and a UMI count matrix was generated using umi tools
SECTION: Analysis
6. We performed analysis of the count matrices using Seurat v2.3.4 in R v3.4.3 (R Core Team, 2013).
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This protocol described the procedures of analyzing RNA-Seq data for the expression of long non-coding RNAs (lncRNAs) as well as protein-coding genes (PCGs), as well as identifying the genomic location of lncRNAs and lncRNA-PCG pairs. As an example, we used an existing RNA-Seq dataset generated in our labotratory, which were  deposited in Gene Expression Omnibus (GEO) database (accession number: GSE101650) and are available to the public. </p>
<p> </p>

[STEPS]
?.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
This protocol is for staining with AbSeq and multiplexing 16 different samples from distinct individuals into the same 10x run. 

[GUIDELINES]
Make all necessary preparations for the experiment:
Notify the IHG core that the 10x Controller will be used.
Book the Attune NXT for cell counting.
Find the samples the day before (Day 0) and place them in a separate box for easy access the following morning.
Keep frozen PBMC vials on dry ice and thawed cells on watery ice when not in use.
Days do not have to be in order. In general, DNA can be kept at 4°C for < 72 hours and -20°C for < 1 week.

[STEPS]
SECTION: Prepare antibody pool
?. 
SECTION: Thaw Frozen PBMCs
?. 
SECTION: Thaw Frozen PBMCs
?. 
SECTION: Thaw Frozen PBMCs
?. 
SECTION: Thaw Frozen PBMCs
?. 
SECTION: Thaw Frozen PBMCs
?. 
SECTION: Thaw Frozen PBMCs
?. 
SECTION: Thaw Frozen PBMCs
?. 
SECTION: DNAse Treatment
?. 
SECTION: DNAse Treatment
?. 
SECTION: DNAse Treatment
?. 
SECTION: DNAse Treatment
?. 
SECTION: DNAse Treatment
?. 
SECTION: Counting on the Attune Flow Cytometer
?. 
SECTION: Counting on the Attune Flow Cytometer
?. 
SECTION: Counting on the Attune Flow Cytometer
?. 
SECTION: Counting on the Attune Flow Cytometer
?. 
SECTION: Counting on the Attune Flow Cytometer
?. 
SECTION: Counting on the Attune Flow Cytometer
?. 
SECTION: Pooling and Staining
?. 
SECTION: Pooling and Staining
?. 
SECTION: Pooling and Staining
?. 
SECTION: Pooling and Staining
?. 
SECTION: Pooling and Staining
?. 
SECTION: Preparing Single Cell Suspension
?. 
SECTION: Preparing Single Cell Suspension
?. 
SECTION: Preparing Single Cell Suspension
?. 
SECTION: Preparing Single Cell Suspension
?. 
SECTION: Preparing Single Cell Suspension
?. 
SECTION: Preparing Single Cell Suspension
?. 
SECTION: Preparing Single Cell Suspension
?.