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[DESCRIPTION]
This is the synthesis protocol for modified nucleotides using the&nbsp;HiScribe&trade;&nbsp;T7 Quick High Yield RNA Synthesis Kit (E2050). The kit is capable of synthesizing biotin- or dye-modified RNA.

[GUIDELINES]
We strongly recommend wearing gloves and using nuclease-free tubes and reagents to avoid RNase contamination. Reactions are typically 20 &mu;l but can be scaled up as needed. Reactions should be assembled in nuclease-free microfuge tubes or PCR strip tubes.<br /><br />The kit is capable of synthesizing biotin- or dye-modified RNA with the following protocol. The recommended molar ratio of modified NTP (Biotin-, Fluorescein-, Digoxigenin-, or Aminoallyl-NTP) to standard NTP is 1:2. The following reaction set-up assumes modified UTP is used. Please note that Dye- or Biotin-NTPs are not supplied with the kit.<br /><br /><strong>Figure 1</strong> shows the time course of labeled RNA synthesis using 1 &micro;g control template with Biotin-16-UTP and Fluorescein-12-UTP following the above reaction setup.<br /><br />Modified ribonucleotides reduce transcription efficiency; therefore, lower transcription yields should be expected as compared to transcription using unmodified NTPs. In general, Biotin-NTP and Aminoallyl-NTP have an insignificant effect on yields, while lower yields can be expected for transcription reactions containing Fluorescein-NTP or Cy-NTP. In addition, transcripts containing modified ribonucleotides have reduced electrophoretic mobility due to higher molecular weight.<br /><br /><img 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' alt='' /><br /><strong>Figure 1. RNA synthesis with modified nucleotides</strong><br /><em>Reactions were incubated at 37&deg;C in a thermocycler. Transcripts were purified by&nbsp;spin columns and quantified on a NanoDrop Spectrophotometer.</em>

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