TITLE: No Title
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[DESCRIPTION]
The kit formulation allows for efficient capped RNA synthesis using cap analog (ARCA).

[BEFORE_START]
We strongly recommend wearing gloves and using nuclease-free tubes and reagents to avoid RNase contamination. Reactions are typically 20 &mu;l but can be scaled up as needed. Reactions should be assembled in nuclease-free microfuge tubes or PCR strip tubes.

[GUIDELINES]
We strongly recommend wearing gloves and using nuclease-free tubes and reagents to avoid RNase contamination. Reactions are typically 20 &mu;l but can be scaled up as needed. Reactions should be assembled in nuclease-free microfuge tubes or PCR strip tubes.<br /><br />The kit formulation allows for efficient capped RNA synthesis using cap analog (ARCA). The recommended ratio of cap analog to GTP is 4:1. Increasing the ratio of cap analog to GTP will increase the proportion of capped RNA transcripts; however, it also significantly decreases the yield of the reaction. Cap analogs are sold separately. Please refer to the&nbsp;<a href='https://www.neb.com/products/e2050-hiscribe-t7-quick-high-yield-rna-synthesis-kit#tabselect2' target='_blank'>companion products</a>&nbsp;section.<br /><br />Cap analog (ARCA,&nbsp;<a href='https://www.neb.com/products/s1411-3-0-me-m7g5ppp5g-rna-cap-structure-analog'>NEB #S1411</a>) is supplied in a lyophilized form of 1 &micro;mol per tube. Dissolving it in 25 &mu;l nuclease-free water will yield a concentration of 40 mM.<br /><br />The yield per reaction is 30&ndash;40 &mu;g RNA with approximately 80% capped RNA transcripts. Figure 1 shows the time course of capped RNA synthesis from 1 &micro;g control template. Most reactions will be complete in 1 hour.<br /><img 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' alt='' /><br /><br /><strong>Figure 1. Capped RNA Synthesis with ARCA</strong><br /><em>Reactions were incubated at 37&deg;C in a thermocycler. Transcripts were purified by&nbsp;spin columns and quantified on a NanoDrop Spectrophotometer.</em>

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