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[GUIDELINES]
<p>The following is a list of antibody concentrations for use with the protocol 'Immunohistochemistry - Drosophila Embryos'<br />Custom antibodies were provided by the published authors.<br /><br />DSHB Antibodies</p>
<table style="border-collapse: collapse; width: 853pt;" border="0" width="853" cellspacing="0" cellpadding="0">
<tbody>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; width: 114pt;" width="114" height="15">Antigen</td>
<td class="xl64" style="border-left: none; width: 69pt;" width="69">Host Species</td>
<td class="xl64" style="border-left: none; width: 168pt;" width="168">Product ID</td>
<td class="xl65" style="border-left: none; width: 77pt;" width="77">Concentration</td>
<td class="xl64" style="border-left: none; width: 425pt;" width="425">Product Link</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">DE-Cadherin</td>
<td class="xl64" style="border-top: none; border-left: none;">rat</td>
<td class="xl64" style="border-top: none; border-left: none;">DCAD2</td>
<td class="xl65" style="border-top: none; border-left: none;">1:16</td>
<td class="xl67" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/cadherin-DE-</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Coracle</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">50:50 mix of C566.9 and C615.16</td>
<td class="xl65" style="border-top: none; border-left: none;">1:50</td>
<td class="xl66" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/Coracle and http://dshb.biology.uiowa.edu/Coracle_2</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Engrailed </td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">4D9</td>
<td class="xl65" style="border-top: none; border-left: none;">1:10</td>
<td class="xl67" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/4D9-anti-engrailed-invected</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Alpha-spectrin</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">3A9</td>
<td class="xl65" style="border-top: none; border-left: none;">1:16</td>
<td class="xl67" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/3A9-323-or-M10-2_2</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Mmp</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;"> 3A6B4</td>
<td class="xl65" style="border-top: none; border-left: none;">1:20</td>
<td class="xl67" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/3A6B4</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Hindsight</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">1G9</td>
<td class="xl65" style="border-top: none; border-left: none;">1:20</td>
<td class="xl67" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/hindsight-protein</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Atp-alpha</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">a5</td>
<td class="xl65" style="border-top: none; border-left: none;">1:50</td>
<td class="xl67" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/a5</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Nervana</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">Nrv5F7</td>
<td class="xl65" style="border-top: none; border-left: none;">1:50</td>
<td class="xl67" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/Nervana-protein</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Discs Large</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">4F3</td>
<td class="xl65" style="border-top: none; border-left: none;">1:10</td>
<td class="xl67" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/4F3-anti-discs-large</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Fasciclin III</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">7G10</td>
<td class="xl65" style="border-top: none; border-left: none;">1:100</td>
<td class="xl68" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/7G10-anti-Fasciclin-III_2</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Crumbs (concentrate)</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">Cq4-c</td>
<td class="xl65" style="border-top: none; border-left: none;">1:50</td>
<td class="xl68" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/Crumbs</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Armadillo</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">N2 7A1</td>
<td class="xl65" style="border-top: none; border-left: none;">1:50</td>
<td class="xl68" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/N2-7A1-ARMADILLO</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Neuroglian</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">BP 104</td>
<td class="xl65" style="border-top: none; border-left: none;">1:25</td>
<td class="xl68" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/neuroglian</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Flamingo</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">Flamingo #74</td>
<td class="xl65" style="border-top: none; border-left: none;">1:20</td>
<td class="xl68" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/Flamingo-74</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">N-Cadherin</td>
<td class="xl64" style="border-top: none; border-left: none;">rat</td>
<td class="xl64" style="border-top: none; border-left: none;">DN-Ex #8</td>
<td class="xl65" style="border-top: none; border-left: none;">1:20</td>
<td class="xl68" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/cadherin-DN-</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Rho1</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">p1D9</td>
<td class="xl65" style="border-top: none; border-left: none;">1:40</td>
<td class="xl68" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/p1D9-anti-rho1</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Alpha-catenin</td>
<td class="xl64" style="border-top: none; border-left: none;">rat</td>
<td class="xl64" style="border-top: none; border-left: none;">DCAT-1</td>
<td class="xl65" style="border-top: none; border-left: none;">1:10</td>
<td class="xl68" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/catenin-alpha-</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Draper</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">Draper 8A1</td>
<td class="xl65" style="border-top: none; border-left: none;">1:25</td>
<td class="xl68" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/Draper8A1</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Delta</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">C594.9B</td>
<td class="xl65" style="border-top: none; border-left: none;">1:50</td>
<td class="xl68" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/Delta-extracellular-domain</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Elav</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">ELAV-9F8A9</td>
<td class="xl65" style="border-top: none; border-left: none;">1:100</td>
<td class="xl68" style="border-top: none; border-left: none;">http://dshb.biology.uiowa.edu/Elav-9F8A9</td>
</tr>
</tbody>
</table>
<p><br />Custom Antibodies<br /><br /></p>
<table style="border-collapse: collapse; width: 574px; height: 382px;" border="0" width="410" cellspacing="0" cellpadding="0">
<tbody>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; width: 90pt;" width="90" height="15">Antigen</td>
<td class="xl64" style="border-left: none; width: 69pt;" width="69">Host Species</td>
<td class="xl64" style="border-left: none; width: 65pt;" width="65">Product ID</td>
<td class="xl65" style="border-left: none; width: 77pt;" width="77">Concentration</td>
<td class="xl64" style="border-left: none; width: 109pt;" width="109">Citation - Pubmed ID</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Pio-Pio</td>
<td class="xl64" style="border-top: none; border-left: none;">rabbit</td>
<td class="xl64" style="border-top: none; border-left: none;">Custom</td>
<td class="xl65" style="border-top: none; border-left: none;">1:100</td>
<td class="xl64" style="border-top: none; border-left: none;" align="right">12973360</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Neuroglian (1B7)</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">Custom</td>
<td class="xl65" style="border-top: none; border-left: none;">1:10</td>
<td class="xl64" style="border-top: none; border-left: none;">                          2805067</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">sqh-1P</td>
<td class="xl64" style="border-top: none; border-left: none;">guinea pig</td>
<td class="xl64" style="border-top: none; border-left: none;">Custom</td>
<td class="xl65" style="border-top: none; border-left: none;">1:250</td>
<td class="xl64" style="border-top: none; border-left: none;" align="right">20920606</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">sqh-2P</td>
<td class="xl64" style="border-top: none; border-left: none;">rat </td>
<td class="xl64" style="border-top: none; border-left: none;">Custom</td>
<td class="xl65" style="border-top: none; border-left: none;">1:500</td>
<td class="xl64" style="border-top: none; border-left: none;" align="right">20920606</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Mcr</td>
<td class="xl64" style="border-top: none; border-left: none;">guinea pig</td>
<td class="xl64" style="border-top: none; border-left: none;">Custom</td>
<td class="xl65" style="border-top: none; border-left: none;">1:400</td>
<td class="xl64" style="border-top: none; border-left: none;" align="right">24496625</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Contactin</td>
<td class="xl64" style="border-top: none; border-left: none;">guinea pig</td>
<td class="xl64" style="border-top: none; border-left: none;">Custom</td>
<td class="xl65" style="border-top: none; border-left: none;">1:2000</td>
<td class="xl64" style="border-top: none; border-left: none;" align="right">15459097</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Kune</td>
<td class="xl64" style="border-top: none; border-left: none;">rabbit</td>
<td class="xl64" style="border-top: none; border-left: none;">Custom</td>
<td class="xl65" style="border-top: none; border-left: none;">1:1000</td>
<td class="xl64" style="border-top: none; border-left: none;" align="right">20407131</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Neurexin</td>
<td class="xl64" style="border-top: none; border-left: none;">rabbit</td>
<td class="xl64" style="border-top: none; border-left: none;">Custom</td>
<td class="xl65" style="border-top: none; border-left: none;">1:500</td>
<td class="xl64" style="border-top: none; border-left: none;">                          8978610</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Uninflatable</td>
<td class="xl64" style="border-top: none; border-left: none;">guinea pig</td>
<td class="xl64" style="border-top: none; border-left: none;">Custom</td>
<td class="xl65" style="border-top: none; border-left: none;">1:400</td>
<td class="xl64" style="border-top: none; border-left: none;" align="right">19818339</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Mtf</td>
<td class="xl64" style="border-top: none; border-left: none;">guinea pig</td>
<td class="xl64" style="border-top: none; border-left: none;">Custom</td>
<td class="xl65" style="border-top: none; border-left: none;">1:500</td>
<td class="xl64" style="border-top: none; border-left: none;" align="right">20935638</td>
</tr>
<tr style="height: 15.0pt;">
<td class="xl64" style="height: 15.0pt; border-top: none;" height="15">Squash</td>
<td class="xl64" style="border-top: none; border-left: none;">mouse</td>
<td class="xl64" style="border-top: none; border-left: none;">Custom</td>
<td class="xl65" style="border-top: none; border-left: none;">1:400</td>
<td class="xl64" style="border-top: none; border-left: none;" align="right">20920606</td>
</tr>
</tbody>
</table>
<p> </p>
<p>Vendor Specific Antibodies - HeLa Cell Concentrations</p>
<table style="width: 574px; height: 382px; border-collapse: collapse;" border="0" width="410" cellspacing="0" cellpadding="0">
<tbody>
<tr style="height: 20px;">
<td class="xl64" style="width: 90pt; height: 20px;" width="90">Antigen</td>
<td class="xl64" style="width: 69pt; height: 20px; border-left-color: currentColor; border-left-width: medium; border-left-style: none;" width="69">Host Species</td>
<td class="xl64" style="width: 65pt; height: 20px; border-left-color: currentColor; border-left-width: medium; border-left-style: none;" width="65">Product ID</td>
<td class="xl65" style="width: 77pt; height: 20px; border-left-color: currentColor; border-left-width: medium; border-left-style: none;" width="77">Concentration</td>
<td class="xl64" style="width: 109pt; height: 20px; border-left-color: currentColor; border-left-width: medium; border-left-style: none;" width="109">Citation - Pubmed ID</td>
</tr>
<tr style="height: 15pt;">
<td class="xl64" style="height: 15pt; border-top-color: currentColor; border-top-width: medium; border-top-style: none;">LC3 (clone 5F10)</td>
<td class="xl64" style="border-top-color: currentColor; border-left-color: currentColor; border-top-width: medium; border-left-width: medium; border-top-style: none; border-left-style: none;">mouse</td>
<td class="xl64" style="border-top-color: currentColor; border-left-color: currentColor; border-top-width: medium; border-left-width: medium; border-top-style: none; border-left-style: none;">0231-100/LC3-5F10</td>
<td class="xl65" style="border-top-color: currentColor; border-left-color: currentColor; border-top-width: medium; border-left-width: medium; border-top-style: none; border-left-style: none;">1:100</td>
<td class="xl64" style="border-top-color: currentColor; border-left-color: currentColor; border-top-width: medium; border-left-width: medium; border-top-style: none; border-left-style: none;" align="right"> </td>
</tr>
<tr style="height: 20px;">
<td class="xl64" style="height: 20px; border-top-color: currentColor; border-top-width: medium; border-top-style: none;"> </td>
<td class="xl64" style="height: 20px; border-top-color: currentColor; border-left-color: currentColor; border-top-width: medium; border-left-width: medium; border-top-style: none; border-left-style: none;"> </td>
<td class="xl64" style="height: 20px; border-top-color: currentColor; border-left-color: currentColor; border-top-width: medium; border-left-width: medium; border-top-style: none; border-left-style: none;"> </td>
<td class="xl65" style="height: 20px; border-top-color: currentColor; border-left-color: currentColor; border-top-width: medium; border-left-width: medium; border-top-style: none; border-left-style: none;"> </td>
<td class="xl64" style="height: 20px; border-top-color: currentColor; border-left-color: currentColor; border-top-width: medium; border-left-width: medium; border-top-style: none; border-left-style: none;">                          2805067</td>
</tr>
</tbody>
</table>
<p> </p>

[STEPS]