TITLE: No Title
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[DESCRIPTION]
Sequence for barcoded oligos for linker amplified libraries. Numbers 1 to 29 were developed at the Tucson Marine Phage Lab dervied from MID 1 to 14 recommended by Roche.

[GUIDELINES]
<strong>Rules for oligo creation:</strong> <br />- No duplicate nucleotides in a row, including ends next to Linker A &amp; emPCR primer<br /> - Barcodes must differ by at least 2 nucleotides<br /> - Linker A has C at 5’ end, so no C at 3’ end of barcode <br />- emPCR primer has G at 3’ end, so no G at 5’ end of barcode <br />- Make with 5’ phosphorylation.<br /><br />5’phos-[5bp Bar Code]-CCACACAGATCACGAAGCATAC-3’<br /><br />
<table style="width: 200px;">
<tbody>
<tr>
<td><strong>1</strong></td>
<td>C</td>
<td>G</td>
<td>A</td>
<td>C</td>
<td>A</td>
</tr>
<tr>
<td><strong>2</strong></td>
<td>C</td>
<td>G</td>
<td>T</td>
<td>G</td>
<td>T</td>
</tr>
<tr>
<td><strong>3</strong></td>
<td>C</td>
<td>T</td>
<td>C</td>
<td>T</td>
<td>A</td>
</tr>
<tr>
<td><strong>4</strong></td>
<td>C</td>
<td>T</td>
<td>A</td>
<td>T</td>
<td>G</td>
</tr>
<tr>
<td><strong>5</strong></td>
<td>C</td>
<td>T</td>
<td>G</td>
<td>A</td>
<td>G</td>
</tr>
<tr>
<td><strong>6</strong></td>
<td>C</td>
<td>A</td>
<td>T</td>
<td>A</td>
<td>G</td>
</tr>
<tr>
<td><strong>7</strong></td>
<td>C</td>
<td>A</td>
<td>G</td>
<td>A</td>
<td>T</td>
</tr>
<tr>
<td><strong>8</strong></td>
<td>A</td>
<td>C</td>
<td>A</td>
<td>G</td>
<td>A</td>
</tr>
<tr>
<td><strong>9</strong></td>
<td>A</td>
<td>C</td>
<td>G</td>
<td>T</td>
<td>G</td>
</tr>
<tr>
<td><strong>10</strong></td>
<td>A</td>
<td>C</td>
<td>T</td>
<td>C</td>
<td>T</td>
</tr>
<tr>
<td><strong>11</strong></td>
<td>A</td>
<td>G</td>
<td>C</td>
<td>A</td>
<td>T</td>
</tr>
<tr>
<td><strong>12</strong></td>
<td>A</td>
<td>T</td>
<td>A</td>
<td>C</td>
<td>T</td>
</tr>
<tr>
<td><strong>13</strong></td>
<td>A</td>
<td>T</td>
<td>C</td>
<td>A</td>
<td>G</td>
</tr>
<tr>
<td><strong>14</strong></td>
<td>A</td>
<td>T</td>
<td>G</td>
<td>T</td>
<td>A</td>
</tr>
<tr>
<td><strong>15</strong></td>
<td>T</td>
<td>G</td>
<td>A</td>
<td>G</td>
<td>T</td>
</tr>
<tr>
<td><strong>16</strong></td>
<td>T</td>
<td>G</td>
<td>C</td>
<td>T</td>
<td>G</td>
</tr>
<tr>
<td><strong>17</strong></td>
<td>T</td>
<td>C</td>
<td>G</td>
<td>C</td>
<td>A</td>
</tr>
<tr>
<td><strong>18</strong></td>
<td>T</td>
<td>C</td>
<td>A</td>
<td>T</td>
<td>A</td>
</tr>
<tr>
<td><strong>19</strong></td>
<td>T</td>
<td>C</td>
<td>T</td>
<td>G</td>
<td>A</td>
</tr>
<tr>
<td><strong>20</strong></td>
<td>T</td>
<td>A</td>
<td>C</td>
<td>G</td>
<td>A</td>
</tr>
<tr>
<td><strong>21</strong></td>
<td>T</td>
<td>A</td>
<td>T</td>
<td>C</td>
<td>G</td>
</tr>
<tr>
<td><strong>22</strong></td>
<td>C</td>
<td>G</td>
<td>C</td>
<td>G</td>
<td>A</td>
</tr>
<tr>
<td><strong>23</strong></td>
<td>C</td>
<td>T</td>
<td>C</td>
<td>A</td>
<td>T</td>
</tr>
<tr>
<td><strong>24</strong></td>
<td>C</td>
<td>A</td>
<td>T</td>
<td>C</td>
<td>A</td>
</tr>
<tr>
<td><strong>25</strong></td>
<td>A</td>
<td>G</td>
<td>A</td>
<td>C</td>
<td>A</td>
</tr>
<tr>
<td><strong>26</strong></td>
<td>A</td>
<td>G</td>
<td>A</td>
<td>G</td>
<td>T</td>
</tr>
<tr>
<td><strong>27</strong></td>
<td>A</td>
<td>G</td>
<td>T</td>
<td>C</td>
<td>A</td>
</tr>
<tr>
<td><strong>28</strong></td>
<td>T</td>
<td>C</td>
<td>G</td>
<td>A</td>
<td>T</td>
</tr>
<tr>
<td><strong>29</strong></td>
<td>T</td>
<td>A</td>
<td>T</td>
<td>A</td>
<td>T</td>
</tr>
</tbody>
</table>
<br />
<table style="width: 200px;">
<tbody>
<tr>
<td><strong>MID 1</strong></td>
<td>A</td>
<td>C</td>
<td>G</td>
<td>A</td>
<td>G</td>
<td>T</td>
<td>G</td>
<td>C</td>
<td>G</td>
<td>T</td>
</tr>
<tr>
<td><strong>MID 2</strong></td>
<td>A</td>
<td>C</td>
<td>G</td>
<td>C</td>
<td>T</td>
<td>C</td>
<td>G</td>
<td>A</td>
<td>C</td>
<td>A</td>
</tr>
<tr>
<td><strong>MID 3</strong></td>
<td>A</td>
<td>G</td>
<td>A</td>
<td>C</td>
<td>G</td>
<td>C</td>
<td>A</td>
<td>C</td>
<td>T</td>
<td>C</td>
</tr>
<tr>
<td><strong>MID 4</strong></td>
<td>A</td>
<td>G</td>
<td>C</td>
<td>A</td>
<td>C</td>
<td>T</td>
<td>G</td>
<td>T</td>
<td>A</td>
<td>G</td>
</tr>
<tr>
<td><strong>MID 5</strong></td>
<td>A</td>
<td>T</td>
<td>C</td>
<td>A</td>
<td>G</td>
<td>A</td>
<td>C</td>
<td>A</td>
<td>C</td>
<td>G</td>
</tr>
<tr>
<td><strong>MID 6</strong></td>
<td>A</td>
<td>T</td>
<td>A</td>
<td>T</td>
<td>C</td>
<td>G</td>
<td>C</td>
<td>G</td>
<td>A</td>
<td>G</td>
</tr>
<tr>
<td><strong>MID 7</strong></td>
<td>C</td>
<td>G</td>
<td>T</td>
<td>G</td>
<td>T</td>
<td>C</td>
<td>T</td>
<td>C</td>
<td>T</td>
<td>A</td>
</tr>
<tr>
<td><strong>MID 8</strong></td>
<td>C</td>
<td>T</td>
<td>C</td>
<td>G</td>
<td>C</td>
<td>G</td>
<td>T</td>
<td>G</td>
<td>T</td>
<td>C</td>
</tr>
<tr>
<td><strong>MID 9</strong></td>
<td>T</td>
<td>A</td>
<td>G</td>
<td>T</td>
<td>A</td>
<td>T</td>
<td>C</td>
<td>A</td>
<td>G</td>
<td>C</td>
</tr>
<tr>
<td><strong>MID 10</strong></td>
<td>T</td>
<td>C</td>
<td>T</td>
<td>C</td>
<td>T</td>
<td>A</td>
<td>T</td>
<td>G</td>
<td>C</td>
<td>G</td>
</tr>
<tr>
<td><strong>MID 11</strong></td>
<td>T</td>
<td>G</td>
<td>A</td>
<td>T</td>
<td>A</td>
<td>C</td>
<td>G</td>
<td>T</td>
<td>C</td>
<td>T</td>
</tr>
<tr>
<td><strong>MID 12</strong></td>
<td>T</td>
<td>A</td>
<td>C</td>
<td>T</td>
<td>G</td>
<td>A</td>
<td>G</td>
<td>C</td>
<td>T</td>
<td>A</td>
</tr>
<tr>
<td><strong>MID 13</strong></td>
<td>C</td>
<td>A</td>
<td>T</td>
<td>A</td>
<td>G</td>
<td>T</td>
<td>A</td>
<td>G</td>
<td>T</td>
<td>G</td>
</tr>
<tr>
<td><strong>MID 14</strong></td>
<td>C</td>
<td>G</td>
<td>A</td>
<td>G</td>
<td>A</td>
<td>G</td>
<td>A</td>
<td>T</td>
<td>A</td>
<td>C</td>
</tr>
</tbody>
</table>
<br /><br />

[STEPS]