eamag/protocols-with-steps huggingface dataset:
TITLE: Fabrication and characterization of a pixel pressure detector using 3-D printable materials or classical PCB materials
AUTHORS: German Sanchez

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol presents the fabrication process for a piezoelectric ( 3D printed or PCB) pixel pressure sensor using commercially available materials.</div></div>

[STEPS]
?. [3D OR PCB PIXEL DETEXCTOR DESIGN]
Into a 3D designer. In this case, inventor, generate an initial base of 38 mm * 38 mm with a thickness of 0.7 mm asshown:
?. [3D OR PCB PIXEL DETEXCTOR DESIGN]
Create a different piece (the one that is expected to be printed in a conductive material), generating pixels of an area of 16 mm^2 , height of 2.1 mm symmetrically spaced (distance of 2.8 mm among them). Then generate paths of 1.4 mm of height and width of 1.4 mm. Each path goes to the border of the figure that is inscribed into a square of 38mm*38mm.
?. [3D OR PCB PIXEL DETEXCTOR DESIGN]
Finally generate a top layer to cover the pixels, as shown here:The squares are spaced 2.8 mm. The height of the holes is 2.1 mm and the height for the paths holes is 1.4 mm.
?. [3D OR PCB PIXEL DETEXCTOR DESIGN]
As a final step to get this structure, align the pieces as shown:
?. [3D OR PCB PIXEL DETEXCTOR DESIGN]
With the two options, the 3D or the PCB inspired one, ask a clean room assistant to follow this protocol and add a 100 nm nanocompiste layer on the structure you've chosen before: Carlos A Perez-Lopez, J.A Perez-Taborda, Alba Avila.Fabrication of a ZnO/PDMS Piezoelectric Triboelectric Nanogenerator.protocols.iodx.doi.org/10.17504/protocols.io.zj8f4rwAt this step, the possible results should look like this:
?. [3D OR PCB PIXEL DETEXCTOR DESIGN]
Finally, its required to add a conductive layer. There are two options as well: 1. Use an (Au) layer of 200 nm over the nanocomposite layer:2. Add aluminium tape (firt remove the glue with alcohol) to get the results as shown:
?. [Sensors Characterization]
Set up a stage for measurements as shown in the figure 11: Materials: * Power supply *Arduino Uno * Motor DC Crouzet 60Follow the next schematic to connect arduino: Place the sensor below the impact area of the stage:
?. [Sensors Characterization]
Connect the oscilloscope terminals to either one pixel PCB copper terminal or a electrifi filament if using the 3D sensor, and the other terminal to the second electrode of the sensor (conductive tape/ gold film).
?. [Sensors Characterization]
Run the code hosted at https://github.com/gasanchez10/Zno-NanocompositeYou can either set the frequency of the stage and the power it uses.
?. [Sensors Characterization]
Finally check the data on the oscilloscope's screen. Register the data and start data processing.
TITLE: Quality control and assurance for S. Typhi amplicon sequencing 
AUTHORS: Jaspreet Mahindroo, Catherine Troman, Anton Spadar, Zoe Dyson, Kathryn Holt, Nick Grassly

[DESCRIPTION]
The following quality control points should be followed alongside the amplicon sequencing protocol for genotyping Salmonella Typhi and help to ensure the protocol has been performed correctly and the results are valid.

[STEPS]
TITLE: Human Kidney, Urinary Tract, and Lung Cell Type Mapping  Pipeline for the Human Biomolecular Atlas Program (HuBMAP)
AUTHORS: Blue Lake, Elizabeth Duong, Dinh H. Diep, Xin Sun, Peter K, Jim Hagood, Gloria Pryhuber, Sanjay Jain, Kun Zhang [University Of California

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This document provides an overview of the protocols used by the Human Kidney, Urinary Tract, and Lung Mapping Center (KULMAP) for generation of spatially resolved mulitomic cell type organ maps within HuBMAP. This involves sequencing of the transcriptomes and epigenomes of dissociated single cells in a massively parallel manner, which will then inform on a highly multiplexed RNA </span><span style = "font-style:italic;">in situ</span><span> imaging method (DART-FISH) for spatial mapping of hundreds of molecular targets in the tissue sections, at a subcellular resolution. These spatial molecular maps will serve as scaffolds for computational registration of cell types and the associated transcriptome/chromatin maps to the tissue space. </span></div></div>

[STEPS]
?. Collection and processing of human tissues.Bladder, Ureter, Kidney: dx.doi.org/10.17504/protocols.io.568g9hwLung airways and parenchyma: dx.doi.org/10.17504/protocols.io.bjuxknxn and dx.doi.org/10.17504/protocols.io.bjtnknme
?. Isolation of single nuclei for omic assays.Bladder, Ureter, Kidney: dx.doi.org/10.17504/protocols.io.ufketkwLung airways and parenchyma: dx.doi.org/10.17504/protocols.io.bh26j8he
?. Running of multimodal single cell/nucleus assays.10X snRNA-seq: dx.doi.org/10.17504/protocols.io.86khzcw10X snATAC-seq: dx.doi.org/10.17504/protocols.io.bvssn6eeSNARE-Seq2: dx.doi.org/10.17504/protocols.io.be5gjg3w
?. Spatial in situ mapping of multiomic profiles.DART-FISH: Protocols coming soon.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
This collection presents some of the most widely used methodological approaches for isolation and purification of bacteriophages and cyanophages, the first step in detailed studies of virus–host interactions and viral genetic composition, and discusses the applications and limitations of different isolation procedures. Most work on phage isolation has been carried out with aerobic heterotrophic bacteria and cyanobacteria, culturable both on agar plates and in enriched liquid cultures. The procedures presented here are limited to lytic viruses infecting such hosts. In addition to the isolation procedures, methods for life cycle characterization (one-step growth experiments) of bacteriophages and cyanophages are described.

[STEPS]
TITLE: Isolation and Transfection of Nicotiana benthamiana Mesophyll Protoplasts for Fluorescent Protein Visualization
AUTHORS: Namitha Nayak, Sandhya Mehrotra, Rajesh Mehrotra

[DESCRIPTION]
Protoplast transfection is routinely used to study the sub-cellular localization of fluorescent-tagged proteins. Using N. benthamiana protoplasts is advantageous as it is a hardy plant that grows well, especially in tropical climates. Isolating protoplasts from the leaves of young 3-4-week-old N. benthamiana plants compensates for the benefits of using the ephemeral Arabidopsis. Moreover, the larger protoplast size of N. benthamiana offers better visualization of fluorescent proteins at lower magnifications. The protocol described here is an easy method for N. benthamiana protoplast isolation and transfection with simple and economical modifications to increase yield and transfection efficiency. The protocol is optimized for easy performance with minimal laboratory equipment. 

[STEPS]
TITLE: UV Exposure Protocol
AUTHORS: Martin O Pollard

[DESCRIPTION]
A procedure utilising a UV crosslinker to expose isolated DNA to UV radiation with the aim of inducing lesions in the DNA typical of that kind of damage induced by UV.

[STEPS]
SECTION: UV treatment
1. Pipette 2.2 µL  of   which has a measured concentration of 227 µg/ml from stock tube to sterile plastic petri dish.
SECTION: UV treatment
2. Dilute material in petri dish with 22.8 µL of buffer to achieve a target of 25 µL of DNA at 20 ng/µl yielding 0.5 µg of DNA. Gently mix but do not agitate vigorously. 

SECTION: UV treatment
3. Expose tube to UV light using Crosslinker at 254 nm to exposing the cells to 800 J/m2 (80000 μJ/cm2)
 

SECTION: UV treatment
3.1. Place sample in Crosslinker drawer
SECTION: UV treatment
3.2. Press the ENERGY button, enter the exposure energy of 80000 microjoules/cm2 as 80 on the keypad.
Note: YOUR ENERGY EXPOSURE SETTINGS DISPLAYED MUST BE MULTIPLIED BY 
100 to obtain the exposure in microjoules/cm2. If settings are correct, push ENTER on the touch pad. 
SECTION: UV treatment
3.3. Press START button to activate crosslinker and wait for cycle to finish.
SECTION: UV treatment
4. Transfer sample to eppendorf tube.
TITLE: RNA Extraction Protocol for Aurantiochytrium limacinum
AUTHORS: Michael Horowitz, Mariana Rius, Jackie Collier

[STEPS]
?. [Prepare cells]
Start a preculture 4 days prior to extraction by inoculating 5 ml of 790 with a colony of Aurantiochytrium limacinum (ATCC MYA-1381). Incubate overnight at 28 C 171 rpm.Use preculture to inoculate 20 ml of 790 in a 250 ml flask. Culture for three days at 28 C, 171 rpm.
?. [Prepare cells]
Determine volume of culture needed to reach 5*10^7 cells via cell count.
?. [Prepare cells]
Place volume needed into a microcentrifuge tube and centrifuge down at 13000rpm for two minutes at room temperature. Discard supernatant.
?. [Prepare cells]
Place pellet in liquid nitrogen for 3-5 minutes.
?. [Trizol Extraction]
Resuspend pellet in 750ul Trizol lysis buffer and incubate on ice for 1 hour and 30 minutes.
?. [Trizol Extraction]
Add 150ul chloroform in fume hood to the pellet and let sit for 2 minutes.
?. [Trizol Extraction]
Vigorously shake the mixture and centrifuge at 13000rpm for 5 minutes at 4C.
?. [Trizol Extraction]
Pipette upper aqueous layer to a sterile microcentrifuge tube on ice.
?. [Trizol Extraction]
Add an equal volume of >95% ethanol to the upper aqueous layer and mix well.
?. [Monarch Kit RNA Extraction]
Use NEB Monarch Total RNA Miniprep Kit from here. All centrifugation steps should be carried out at 16,000rcf.
?. [Monarch Kit RNA Extraction]
Take an RNA purification column and collection tube. Place the mixture (the upper aqueous layer with ethanol) from the microcentrifuge tube to the purification column.
?. [Monarch Kit RNA Extraction]
Centrifuge for 30 seconds and discard flow-through.
?. [Monarch Kit RNA Extraction]
Add 500ul RNA Wash Buffer and spin for 30 seconds. Discard flow-through.
?. [Monarch Kit RNA Extraction]
In a separate RNase-free microcentrifuge tube, combine 5ul DNase 1 provided in the Monarch Kit with 75ul DNase 1 Reaction Buffer. Pipette the mixture directly onto the column matrix.
?. [Monarch Kit RNA Extraction]
Incubate for 15 minutes at room temperature.
?. [Monarch Kit RNA Extraction]
Add 500ul RNA Priming Buffer and spin for 30 seconds. Discard the flow-through.
?. [Monarch Kit RNA Extraction]
Add 500ul RNA Wash Buffer and spin for 30 seconds. Discard the flow-through.
?. [Monarch Kit RNA Extraction]
Add another 500ul RNA Wash Buffer and spin for two minutes. Transfer the column to a new RNase-free microcentrifuge tube.
?. [Monarch Kit RNA Extraction]
Add 30ul of Nuclease-free Water directly to the column matrix and spin for 30 seconds.
?. [Monarch Kit RNA Extraction]
Pipette the flow-through back into the column matrix and spin again for 30 seconds.
?. [Monarch Kit RNA Extraction]
Remove column matrix, close the microcentrifuge tube, and label it. Store the eluted RNA at -20C for short-term storage or at -80C for long-term storage.RNA yields for GPY grown cells are usually around a 1000ng/ul and for 790-grown cells are much lower around 50-100ng/ul. Lower yield for 790 cells are possibly due to excessive cell clumping.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>A method to transform the chloroplast of <em>Amphidinium carterae</em> using biolistics.</p>
<pre>orcid.org/0000-0002-2469-0611</pre>
<pre>orcid.org/0000-0002-6975-8640</pre>
<pre>orcid.org/0000-0003-4487-196X</pre>
<pre> </pre>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Intact cell cross-linking
AUTHORS: Veerle Baekelandt

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Intact cell cross-linking</span></div></div>

[STEPS]
?. seed cells (SHSY-5Y) in 10 cm dishes at a density of 6 x 10^6 cells per plate.incubate for
?. wash cells 2 times with 1X PBS
?. harvest cells with 1x PBS + PI cocktail (PBS + PI buffer)
?. and resuspend in + PI buffer
Centrifuge: 1000 34, 15 min
400 µl
?. divide  resuspended cells over 2 vialstube 1: add 1 µM disuccinimidyl glutarate (DSG; Thermo Fisher Scientific)tube 2: add 1μM dithiobis succinimidyl propionate (DSP; Thermo Fisher Scientificincubate  at Preparation of 50X DSP, 50X DSG50X DSP: 2.02mg into 100µL DMSO50X DSG: 1.632mg into 100µL DMSONote: Prepare the crosslinkers freshly before use
400 µl
37 °C
?. quenche reaction for  on   in both tubes with  Tris ( )
37 Room temperature
?. sonicate cells 2 times at 30 Hz with 15 ON-OF intervals of  (1s pulses)
TITLE: Small-scale Expression and Solubility Testing of Proteins in BL21 E. coli 
AUTHORS: Vincent Bielinski

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Scaled-down solubility screen for bacterial overexpression constructs. </div></div>

[STEPS]
?. [Screening]
Grow and induce BL21cells in medium of your choice with appropriate temperature and inducer conditions. We often use Teriffic Broth and culture cells at 30 oC, with induction carried out at 18 oC overnight (1mM IPGTG or 0.5% L-arabinose).
?. [Screening]
Harvest 10 mL overnight expression culture by centrifugation for 10 minutes at 3000 x g.
?. [Screening]
Remove supernatant by decanting and resuspend pellet in 800 uL Lysis Buffer. Transfer cell suspension to to a new 1.5 mL Eppendorf tube and store on ice.
?. [Screening]
Sonicate as appropriate for complete cell lysis (settings will depend on make and model of your sonicator).
?. [Screening]
Clarify lysates by centrifugation at 4 oC for 10 minutes at 12-15000 x g.
?. [Screening]
While lysates are spinning, equilibrate the Talon resin by washing 3x with 5 volumes of resin bedding.NOTE: For each pulldown you do, you will need 25 ul of equilibrated Talon resin.This comes supplied as a 50% slurry in ethanol, so calculate how many pulldowns you will be doing and then add 50 uL. If you have 8, estimate you will need enough for 10 pull-downs. To equilibrate, shake the slurry well and remove 500 ul resin slurry with pipette.  Transfer slurry to new Eppendorf tube. Pellet resin by spinning in microfuge for 30 seconds at 3000 x g, then remove supernatant carefully and add 1 mL of Lysis Buffer. Vortex resin briefly, then pellet again and wash beads 2x more with 1 mL of Lysis Buffer. After the third spin, remove supernatant and add 250 uL of Lysis Buffer to produce 500 uL of equilibrated resin.
?. [Screening]
Transfer 750 uL of clarified supernatant to a new Eppendorf tube.
?. [Screening]
Add 50 uL equilibrated resin to each Eppendorf tube and incubate for 1 hr at RT with end-over-end mixing.
?. [Screening]
After 1 hour, pellet resin with 1 min 3000 x g spin at RT, then remove supernatant with piepette.
?. [Screening]
Wash resin 3x with 1 mL of Wash Buffer, pelleting resin between each wash and removing supernatant.
?. [Screening]
After third wash, remove supernatant and resuspend resin in 50 uL of Elution Buffer.
?. [Screening]
Incubate at RT for 5-10 minutes, then quickly spin down beads and transfer eluate to clean Eppendorf tube.
?. [Screening]
Analyze 5-10 uL of eluate on an SDS gel and stain with Coomassie blue.
?. [Screening]
Repeat elution again, combining eluates in single tube at the end (100 uL eluate).
TITLE: It improves your skin and prevents you from acne, pimples etc
AUTHORS: Condor  CBD Gummies

[DESCRIPTION]
Perhaps you will put your own spin on Condor CBD Gummies after this.

[STEPS]
SECTION: It improves your skin and prevents you from acne, pimples etc
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https://wefocile.wixsite.com/condor-cbd-gummies-r
http://fy9.in/QQ7K6Y
SECTION: It improves your skin and prevents you from acne, pimples etc
2. 
3.
TITLE: Crystallisation of Enterovirus coxsackievirus A16 2A protease
AUTHORS: ryan Lithgo, Peter Marples, Lizbé Koekemoer, Daren Fearon

[DESCRIPTION]
Picornaviridae coxsackievirus A16 is the causative agent of paediatric hand-foot-and-mouth disease, and a target for pandemic preparedness due to the risk of higher order complications in a large-scale outbreak. The 2A protease of the virus is responsible for self-cleavage from the poly protein, allowing for correct folding and assembly of capsid proteins in the final stages of viral replication. Inhibition deranges capsid folding and assembly, preventing formation of mature virions in host cells and making the protease a valuable target for antiviral activity. This protocol was used to grow coxsackievirus A16 crystals that were applied high-throughput crystallographic fragment screening on the target.

[STEPS]
SECTION: Crystallisation experiment
4. Protein and buffer requirements:
43.2 µL20 mg/mL 
3.36 mL 
14.4 µL   seeds, dilution 1:1000
SECTION: Crystallisation experiment
6. Dispense 35 µL into SwissCI 3 lens plate reservoir wells using a 100 µl multi-channel pipette.
Dispense 150 nL20 mg/mL  to each lens using the SPT mosquito.
Dispense  150 nL to each lens using the SPT mosquito.
Dispense  50 nL to each lens using the SPT mosquito.

Drop ratio: 3:3:1 ratio (150 nl   : 150 nl reservoir solution: 50 nl seeds)
Final drop volume: 350 nl
SECTION: Crystallisation experiment
5. Crystallisation screen composition:
13.5 % w/v  PEG 20000
0.1 Molarity (M)  MES pH 6.7 


Stock solutions used:
1 Molarity (M)  MES pH 6.7  
50% w/v PEG 20000

 

SECTION: Crystallisation experiment
8. Crystal typically form after ~24hrs
 


SECTION: Crystallisation experiment
7. Incubate at 20 °C for 1440 min in Formulatrix Rock Imager.

Imaging Schedule: The first images are taken after 12hrs and the imaging schedule follows a Fibonacci sequence of days for further collections. 
SECTION: Equipment needed
2. Formulatrix Rock Imager (or incubator of choice)
SPT mosquito
 
P100 8 multi-channel pipette
SwissCI 3 lens plate

SECTION: Crystallisation experiment
3. Prepare seed stock:
 
1: 1000 dilution    seeds
SECTION: Data collection at Synchrotron
9. Diamond Light Source
Unattended Data Collection (UDC)
Data Collection Temperature: 100K
Detector: DECTRIS EIGER2 X 9M
Beamline: I04-1
Wavelength:  0.9212 Å
Resolution (Å): 1.21
Beam Size (μm): 60 X 50
Number of images: 3600
Oscillation: 0.10°
Exposure (s): 0.0020
Transmission (%): 100
Flux (ph/s): 9.50e+11
SECTION: Enterovirus coxsackievirus A16 2A protease expression and purification
1. The protein used for crystallisation was expressed and purified using the following protocol.
TITLE: How to make a cup of tea
AUTHORS: Shiau  Haln Chen, ines.boehm  

[DESCRIPTION]
This protocol is part of the Record Keeping lesson within the FAIR in (biological) practice DataCarpentry course. Here we explore the perfect protocol to brew tea.



[BEFORE_START]
Make sure the kettle is cleaned and the bottom of the kettle is free of lime-scale.
Should there be lime-scale, add small amount of 5% vinegar just covering the base to the kettle and leave over-night, or add about half a cm of 5% vinegar and boil the kettle. The latter should get rid of the lime-scale right away. Any type of malt-vinegar should do the job.

[GUIDELINES]
Brew tea fresh on demand.

[STEPS]
SECTION: Preparation of water and tea
1. Fill the kettle with ~500mL of water, or until your min fill line and boil the water.
SECTION: Preparation of water and tea
2. In the meantime prepare your tea bag, unpack it and place it in the cup/mug so that the tag is positioned outside of the mug.
SECTION: Preparation of water and tea
3. Once the water is boiled, instantly pour the hot water into the mug. Ensure you fix the tag on the outside of the mug with your finger, to avoid falling in of the string + tag of the tea bag. Otherwise removal of the tea bag becomes more difficult.
SECTION: Brewing
4. Brew the tea for 5 min
SECTION: Brewing
5. Use the string/tag to remove the tea bag. Discard the tea bag in your compost for most appropriate recycling.
SECTION: Flavouring
6. Add 5 mL = 1 teaspoon soy milk to your tea
SECTION: Flavouring
7. Enjoy!
TITLE: Tomato Transformation Media
AUTHORS: Alex Rajewski

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol is simply the media recipes for use with my tomato transformation protocol.</div></div>

[STEPS]
?. To make 1L, for each media, dissolve the following components to ~800mL of MilliQ water in a 1L beaker  ABCDEFG1Component:1/2MSOMS0-2%KCMS2Z1ZRooting2MS Basal Salts2.15g4.3g4.3g------3MS Salts with Nitsch Vitamins------4.3g4.3g4.3g4Sucrose10g20g30g20g20g30g5myo-Inositol100mg100mg100mg100mg100mg--6KH2PO4----200mg------7Thiamine HCl (1mg/mL)2mL400µL1.3mL------8Pyridoxine HCl (1mg/mL)500µL500µL--------9Nicotinic Acid (1mg/mL)500µL500µL--------10Glycine--2mg--------112,4-D (1mg/mL)----200µL------12Kinetin (1mg/mL)----100µL------
ABCDEFG1Component:1/2MSOMS0-2%KCMS2Z1ZRooting2MS Basal Salts2.15g4.3g4.3g------3MS Salts with Nitsch Vitamins------4.3g4.3g4.3g4Sucrose10g20g30g20g20g30g5myo-Inositol100mg100mg100mg100mg100mg--6KH2PO4----200mg------7Thiamine HCl (1mg/mL)2mL400µL1.3mL------8Pyridoxine HCl (1mg/mL)500µL500µL--------9Nicotinic Acid (1mg/mL)500µL500µL--------10Glycine--2mg--------112,4-D (1mg/mL)----200µL------12Kinetin (1mg/mL)----100µL------
?. Bring volume to 1L and adjust pH (±0.03) according to the table below:  ABCDEFG11/2MSOMS0-2%KCMS2Z1ZRooting2pH5.85.65.56.06.06.0
ABCDEFG11/2MSOMS0-2%KCMS2Z1ZRooting2pH5.85.65.56.06.06.0
?. Divide the volume into two screw-top jars and add the following gelling agent. Note that not all medias require a gelling agent, and that this mass is the TOTAL mass added for 1L. It is not necessary to dissolve this powder; it will dissolve in the autoclave.  ABCDEFG11/2MSOMS0-2%KCMS2Z1ZRooting2Agar8g----------3Agargel----5.2g5.2g5.2g--4Difco Bacto Agar----------8g
ABCDEFG11/2MSOMS0-2%KCMS2Z1ZRooting2Agar8g----------3Agargel----5.2g5.2g5.2g--4Difco Bacto Agar----------8g
?. Autoclave the media, and allow to cool to ~50º. Add any antibiotics and phytohormones (filter-sterile) according to the table below:  ABCDEFG11/2MSOMS0-2%KCMS2Z1ZRooting2Zeatin (1mg/mL)------2mL1mL--3Timentin (300mg/mL)------1mL1mL--4AntibioticsNoNoNoYesYesMaybe
ABCDEFG11/2MSOMS0-2%KCMS2Z1ZRooting2Zeatin (1mg/mL)------2mL1mL--3Timentin (300mg/mL)------1mL1mL--4AntibioticsNoNoNoYesYesMaybe
?. In the sterile hood, dispense the media into the following containers. The liquid media can simply be stored in the screw-top jar.  ABCDEFG11/2MSOMS0-2%KCMS2Z1ZRooting2100x15mm Petri DishYes--Yes------3100x20mm Petri Dish------Yes----416oz Soup Container--------YesYes
ABCDEFG11/2MSOMS0-2%KCMS2Z1ZRooting2100x15mm Petri DishYes--Yes------3100x20mm Petri Dish------Yes----416oz Soup Container--------YesYes
?. Once the media has solidified, return the plates to their sleeves, label them accordingly, and store a 4ºC for up to several months.