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[DESCRIPTION]
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<div class="column">Highlights<br />
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<p>Quick (15 minute) high-yield recovery of ultra-pure DNA from agarose gels.</p>
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<p>Column design permits DNA elution at high concentrations into minimal volumes (≥ 6 μl).</p>
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<p>Eluted DNA is well suited for use in DNA ligation, sequencing, labeling, PCR, etc.</p>
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[BEFORE_START]
<p><em>Before starting:</em> Add 24 ml 100% ethanol (26 ml 95% ethanol) to the 6 ml <strong>DNA Wash Buffer</strong> concentrate. Add 96 ml 100% ethanol (104 ml 95% ethanol) to the 24 ml <strong>DNA Wash Buffer</strong> concentrate.</p>
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[GUIDELINES]
<p><strong>Product Contents</strong></p>
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<p><img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/gs8c2w6.png" width="450" data-src="https://s3.amazonaws.com/pr-journal/gs8c2w6.png" data-ofn="Screen Shot 2016-11-25 at 10.04.12 AM.png" /></p>
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<p>Integrity of kit components is guaranteed for up to one year from date of purchase. Reagents are routinely tested on a lot-to-lot basis to ensure they provide the highest performance and reliability.</p>
<p><sup>1</sup> Ethanol must be added prior to use as indicated on the <strong>DNA Wash Buffer</strong> label.</p>
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<p><strong>Specifications</strong></p>
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<p><strong>DNA Purity</strong> – High-quality, purified DNA is especially well suited for sequencing and ligation reactions.</p>
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<p><strong>DNA Size Limits</strong> – From ~50 bp to 23 kb.</p>
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<p><strong>DNA Recovery</strong> – Typically, up to 5 μg total DNA per column can be eluted into as little as 6 μl of low salt<strong> DNA Elution Buffer</strong> or water. For DNA 50 bp to 10 kb, the recovery is 70-90%. For DNA 11 kb to 23 kb, the recovery is 50-70%.</p>
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<p><strong>Sample Sources</strong> – DNA in excised agarose gel slices.</p>
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<p><strong>Product Detergent Tolerance</strong> – ≤ 5% Triton X-100, ≤ 5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS.</p>
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<p><strong>Product Description</strong></p>
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<p>The <strong>Zymoclean™ Gel DNA Recovery Kit</strong> provides a hassle-free method for high yield recovery of pure DNA from agarose gels. Simply add the specially formulated <strong>Agarose Dissolving Buffer (ADB)</strong> to the gel slice containing your DNA sample, let dissolve, and then transfer to the supplied <strong>Zymo-Spin™ Column</strong>. There is no need for organic denaturants or chloroform. Instead, the product utilizes Fast-Spin column technology to yield high-quality DNA in just 15 minutes (See figures below). DNA purified using the <strong>Zymoclean™ Gel DNA Recovery Kit</strong> is perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.</p>
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<p><img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/gtbc2w6.png" width="450" data-src="https://s3.amazonaws.com/pr-journal/gtbc2w6.png" data-ofn="Screen Shot 2016-11-25 at 10.08.01 AM.png" /></p>
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<p><strong>Zymoclean™ </strong>products are offered in single column (uncapped or capped column) or 96-well format. In addition, the <strong>Zymoclean™ Large Fragment DNA Recovery Kit</strong> is designed for large DNA (up to 200 kb) gel recovery.</p>
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<p><img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/gtcc2w6.png" width="450" data-src="https://s3.amazonaws.com/pr-journal/gtcc2w6.png" data-ofn="Screen Shot 2016-11-25 at 10.09.09 AM.png" /></p>
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<p><strong>Buffer Preparation</strong></p>
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<p><em>Before starting:</em> Add 24 ml 100% ethanol (26 ml 95% ethanol) to the 6 ml <strong>DNA Wash Buffer</strong> concentrate. Add 96 ml 100% ethanol (104 ml 95% ethanol) to the 24 ml <strong>DNA Wash Buffer</strong> concentrate.</p>
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<p><strong>Troubleshooting</strong></p>
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<p><strong>Low Recovery</strong></p>
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<p><strong>Ensure Agarose is Fully Dissolved</strong><br />There may be small globules of undissolved agarose in the sample that can interfere with DNA recovery by clogging the column and leeching salts into the eluate.</p>
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<p><strong>Gel Dissolved at Temperatures Above 60 °C</strong><br />If dissolved at a higher temperature, DNA may be denatured affecting recovery. For optimal results, dissolve the gel slice between 37-55 °C.</p>
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<p><strong>Improperly Prepared/Stored DNA Wash Buffer</strong><br />Make sure ethanol has been added to the DNA Wash Buffer concentrate. Cap the bottle tightly to prevent evaporation over time.</p>
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<p><strong>Addition of DNA Elution Buffer</strong><br />Add elution buffer directly to the column matrix, not to the walls of the column. Elution buffer requires contact with the matrix for at least 1 minute for large DNA ≥ 10kb.</p>
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<p><strong>Incomplete Elution</strong></p>
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<p>DNA elution is dependent on pH, temperature, and time. For large genomic DNA (≥ 50 kb), apply heated elution buffer (60-70 °C) to the column and incubate for several minutes prior to elution.</p>
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<p>Sequential elutions may be performed for quantitatively higher recovery but lower final DNA concentration. This is recommended for DNA ≥ 10 kb.</p>
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<div class="column"><strong>Low A260/A230 ratio</strong><br />
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<p>When removing the column from the collection tube, be careful that the tip of the column does not come into contact with the flowthrough. Trace amounts of salt from the flowthrough can contaminate a sample resulting in a low A<sub>260</sub>/A<sub>230</sub> ratio. Ethanol contamination from the flowthrough can also interfere with DNA elution. Zymo-Spin™ columns are designed for complete elution with no buffer retention or carryover (see below).</p>
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<p><img class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/gtdc2w6.png" width="450" data-src="https://s3.amazonaws.com/pr-journal/gtdc2w6.png" data-ofn="Screen Shot 2016-11-25 at 10.25.39 AM.png" /></p>
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<p><strong>Following Clean-up with the DCCTM, Multiple Bands Appear in an Agarose Gel</strong></p>
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<li><strong>Acidification of DNA Loading Dye</strong></li>
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<p>Most loading dyes do not contain EDTA and will acidify (pH ≤ 4) over time due to some microbial growth. This low pH is enough to cause DNA degradation. Therefore, if water is used to elute the DNA, 6X Loading Dye containing 1 mM EDTA is recommended.</p>
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