TITLE: Assembling Algal Shaker
AUTHORS: Jakub Nedbal

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol described the final assembly steps to complete the illuminated orbital shaker and its testing. The steps involve fixing the transparent platform to the orbital shaker, placing the LED illuminator underneath, and electrically connecting the parts to the mains supply.</div><div class = "text-block"><span style = "font-weight:bold;">This document is the final part of the Illuminated Orbital Shaker for Microalgae Culture project:</span></div><div class = "text-block"><ul style = "list-style-type:disc;"><li style = "counter-reset:ol0;list-style-type:disc;">Procuring Parts for Algal Shaker</li><li style = "counter-reset:ol0;list-style-type:disc;">Assembling LED Controller Electronics</li><li style = "counter-reset:ol0;list-style-type:disc;">3D Printing Case for LED Controller</li><li style = "counter-reset:ol0;list-style-type:disc;">Assembling Cooled LED Illuminator</li><li style = "counter-reset:ol0;list-style-type:disc;">Cutting and Drilling Clear Acrylic Sheet</li><li style = "counter-reset:ol0;list-style-type:disc;"><span>Assembling Algal Shaker </span><span style = "font-style:italic;">(this document)</span></li></ul></div></div>

[STEPS]
?. Prepare a tidy workbench with sufficient space and illumination to minimize risk of accidents.
?. Fix the four M4×50 mm male-female standoffs to the orbital shaker platform using nuts and washers.Where Imperial threads are preferred, use #8-32×2" stand-offs instead.​
?. Lay the cooled LED illuminator onto the orbital shaker platform, between the standoffs.
?. Place the clear acrylic sheet over the standoffs.
?. Place the antislip mat delivered with the orbital shaker over the clear acrylic sheet. Orient it with the antislip face facing up. Pierce small holes through the antislip mat using a needle or a sharp blade in the locations of the mounting holes.
?. Use M4×12 mm screws and washers to fix the clear acrylic sheet with the antislip mat to the orbital shaker platform.Where Imperial screws are preferred, use #8-32 UNC×1/2" screws and washers instead.
?. Program the 24 hour timer to the desired day/night cycle. In our case the day starts at 6 AM and finishes at 8PM. Set the current time on the 24 hour timer.
?. Connect the 30V/1A power supply into the LED controller power input and plug it into the 24 hour timer.Plug the orbital shaker and the 24 hour timer to the mains socket.
?. Turn on the LED to the desired power setting using the toggle switch and the rotary switch on the LED controller. This may require temporarily pulling out the cooled LED illuminator to reach these switches. Set the spin speed on the orbital shaker to the desired speed (100 rpm, in our case) and turn it on.
?. With the above steps complete, the orbital shaker should keep shaking 24-hours a day and cycle the light with the interval set on the 24 hour timer.
?. This protocol finished the assembly of the illuminated orbital shaker for microalgae culture. The cooled LED illuminator has been installed onto the shaker platform and a new light-transmissive antislip platform was built over the illuminator.
?. This document is the final part of the Illuminated Orbital Shaker for Microalgae Culture project:Procuring Parts for Algal ShakerAssembling LED Controller Electronics3D Printing Case for LED ControllerAssembling Cooled LED IlluminatorCutting and Drilling Clear Acrylic SheetAssembling Algal Shaker (this document)