eamag/protocols-with-steps huggingface dataset:
TITLE: construct a taro linkage map using onemap
AUTHORS:  Michael Kantar, M Renee Bellinger, Roshan Paudel

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">R script for taro linkage mapping using program onemap. </div><div class = "text-block">This script was applied to create a linkage map for a taro mapping population resistant to taro leaf blight. </div><div class = "text-block">The genotype file was constructed by calling SNPs using genotyping by sequencing data set using a taro genome reference genome available from NCBI's genbank under Bioproject PRJNA567267.</div></div>

[STEPS]
?. [Set up your R working environment]
### There are two ways to install onemap###-------------------###### Option 1. ####Download and install Rtools in your computer before running this script###https://cran.r-project.org/bin/windows/Rtoolsfind_rtools(debug = TRUE)###Install required packagesinstall.packages("devtools")library(devtools)### The above installation is supposed to run in R version to 3.5 but I had issue with getting it to install.devtools::install_github("Cristianetaniguti/onemap")library("onemap")###-------------------###### Option 2. If using R 5.6 this package is compatible with the CRAN version### install.packages(onemap)libarary(onemap)install.packages("vcfR")library("vcfR")#######Set working directorysetwd("/your/working/directory/")#####Convert the vcf file into onemap format.## BE SURE THE txt is removed from the end of the vcf file namevcfR.object example_out                                     parent1 = "P230.sam.mapped.bam",                                     parent2 = "P255.sam.mapped.bam",                                     cross = "outcross")####Get the information on marker genotypes.plot(example_out)###Number of markers by segregation typeplot_by_segreg_type(example_out)###Find redundant markersbins bins###Create onemap file after removing redundant markersbins_example bins_example###testing segregation pattern of the markerssegreg_test print(segreg_test)#####to show the markers names with segregation distortionselect_segreg(segreg_test, distorted = TRUE)###to show the markers numbers with segregation distortiondist #to show the markers numbers without segregation distortionno_dist no_dist####See the segregation test graphicallyplot(segreg_test)##calculate a suggested LOD scoreLOD_sug LOD_sug###Estimating two-point recombination fractionstwopts twopts###Create the sequences of markers without segregation distortionmark_no_dist ###Number of linkage groupslg print(lg, detailed = FALSE)###Estimate a genetic mapmapsfor(i in 1:lg$n.groups)  maps[[i]]                                   "rcd"), "force")##Draw the maps##Draw the mapsdraw_map(maps, names = FALSE, grid = TRUE, cex.mrk = 0.7)draw_map2(maps,output="maps.png")####Name the map and extract it into external filefile.outwrite_map(map1, file.out)
TITLE: Gcase co-immunoprecipitation
AUTHORS: michela.deleidi, Federico   Bertoli, María José Pérez J., Hariam Raji

[DESCRIPTION]
We developed this protocol to identify protein-protein interactions between the enzyme glucocerebrosidase (GCase) and other proteins in human iPSC-derived Neural Precursor Cells.

[STEPS]
SECTION: Gcase co-immunoprecipitation
1. Wash cells 1X with phosphate-buffered saline (PBS, Sigma‒Aldrich) and detach using Accutase.
SECTION: Gcase co-immunoprecipitation
2. Pellet the cell suspension at 280 rcf, 5 min, 23 °C.
SECTION: Gcase co-immunoprecipitation
3. Lyse the pellets in IP/lysis buffer (Thermo Fisher, #87787) supplemented with a  protease/phosphatase inhibitor cocktail (Pierce, #A32959). 
SECTION: Gcase co-immunoprecipitation
4. Carry out coimmunoprecipitation using the Thermo Fisher Pierce Crosslink Magnetic IP/Co-IP Kit (#88805) according to the manufacturer´s instructions:
SECTION: Gcase co-immunoprecipitation
5. Prewash 25 µL of  Pierce protein A/G magnetic beads (Thermo Fisher, #88802-3) twice with 1X Modified Coupling Buffer and incubate with 10 µg of GBA MaxPab polyclonal rabbit antibody (Abnova) or normal rabbit IgG (Covalab, #pab01004-P) on a rotating wheel   at4 °C. 
SECTION: Gcase co-immunoprecipitation
6. The following day, crosslink the antibody to the beads with a 0.25 millimolar (mM) DSS solution for 60 min on a rotating wheel at Room temperature.
SECTION: Gcase co-immunoprecipitation
7. Incubate crosslinked magnetic beads 60 min with a total of 7 mg of protein for each lysate. 
SECTION: Gcase co-immunoprecipitation
8. Elute Coimmunoprecipitated proteins from the beads with 60 µL of the kit-provided Elution buffer pH 2.0 (Pierce, #88805) and neutralize with 6 µL of Neutralization Buffer provided with the kit. 
SECTION: Gcase co-immunoprecipitation
9. Prepare samples for Western blotting by adding 5x Lane buffer +10% DTT 1 Molarity (M) to a final concentration of 1X.
TITLE: Agro Transformation for Mimulus in Planta Transformation
AUTHORS: Yaowu Yuan

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol is part of a </span><a style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;">collection for </span><span style = ":;font-style:italic;">Mimulus in planta</span><span style = ":;"> transformation</span></a><span>. </span></div></div>

[STEPS]
?. [Agro Transformation]
Please select from the following two options: 1. Agro Transformation - Using Electroporator2. Agro Transformation - Using Freeze -Thaw Method
?. [Agro Transformation]
Chill 2-mm electroporation cuvette on ice.
?. [Agro Transformation]
Aliquot    into eppie tube.
[LB]
?. [Agro Transformation]
Add  to agro cells.
[plasmid DNA]
?. [Agro Transformation]
Transfer the agro + plasmid DNA to the cuvette.
?. [Agro Transformation]
Electroporate: (Bio-Rad Electroporator program Agr)Capacitance:	25 µFVoltage:	2.4 kV Resistance: 200 Ohm Pulse length: 5 msec
?. [Agro Transformation]
Immediately add  to the cuvette.
[LB]
?. [Agro Transformation]
Transfer	to an eppie tube and shake at - for 1-2 hrs  .
?. [Agro Transformation]
Plate on LB + Kan + Gent + Rif plate and incubate for  at - .
100 µl
?. [Agro Transformation]
Thaw agro competent	cells on ice (stored at )– Agrobacterium GV3101
?. [Agro Transformation]
Thaw competent Agrobacterium on ice (use  per transformation reaction) and add .
250 µl
[standard E. coli miniprep plasmid]
?. [Agro Transformation]
Incubate the mixture on ice for .
?. [Agro Transformation]
Transfer mixture to liquid nitrogen and incubate for .
?. [Agro Transformation]
Incubate the mixture for a further  in a  dry bath.
?. [Agro Transformation]
Add  to each tube.
[LB]
?. [Agro Transformation]
Shake at  for at least .
?. [Agro Transformation]
Collect the cells as follows.
?. [Agro Transformation]
Spin at  for  in a microcentrifuge.
?. [Agro Transformation]
Take  out.
[LB]
?. [Agro Transformation]
Resuspend the pellet with the remaining LB.
?. [Agro Transformation]
Spread the cells on LB agar plates containing the appropriate antibiotic.
?. [Agro Transformation]
Incubate cells for  at .
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This protocol describes briefly how to perform colonization of aposymbiotic Aiptasia with <em>Symbiodinium</em> cultures.</p>
<p> </p>
<p>The method is based on the one described in Xiang et al., 2013.</p>

[BEFORE_START]
<p>The colonizationworks most efficiently with <em>Symbiodinium</em> cultures not older than 2 months. </p>
<p> </p>
<p> </p>
<p>Fluorescence filters:</p>
<p>Every wavelength between 400 and 550 nm efficiently axcites the algal chlorophyll.</p>
<p>The chlorophyll fluorescence emission peaks between 650 and 720 nm.</p>
<p>This means that most RFP filter sets with a wide bandpass or longpass emission filter at above 600 nm will work well.</p>
<p>GFP longpass filter sets will work as well but only in combination with an RGB camera to distinguish red algal fluorescence and green autofluorescence of the anemones themselves.</p>
<p>These numbers are only rough estimations based on a few algal strains. For purchasing new filter sets it will be still best to test them before buying.</p>

[GUIDELINES]
<p>Be careful while working with <em>Symbiodinium</em> cultures to not introduce any contaminations which might overgrow the slow-growing algae. Best practice is to perform all steps involving open cultures under sterile conditions in a clean bench.</p>
<p>The medium of the cultures can be IMK or IMK with casein hydrolysate, if you want to assess the colonization rates of different host or algae strains make sure to use the same medium for all cultures.</p>
<p>A general consideration: The smaller the tank with the anemones, the less <em>Symbiodinium</em> culture is needed.</p>
<p> </p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Cerebral aneurysms are a major risk factor for intracranial bleeding with devastating consequences for the patient. One recently established treatment is the implantation of flow diverters (FD). Methods to predict their treatment success before or directly after implantation are not well investigated yet. The aim of this work was to quantitatively study hemodynamic parameters in patient-specific models of treated cerebral aneurysms and its correlation with the clinical outcome. Hemodynamics were evaluated using both computational fluid dynamics (CFD) and phase contrast (PC) MRI. For preoperative and postoperative distribution of hemodynamic parameters, CFD simulations and PC-MRI velocity measurements showed similar results. In both cases where no occlusion of the aneurysm was observed after six months, a flow reduction of about 30-50% was found, while in the clinically successful case with complete occlusion of the aneurysm after 6 months, the flow reduction was about 80%. No vortex was observed in any of the three models after treatment. The results are in agreement with recent studies suggesting that CFD simulations can predict treatment success or failure already before implantation of an FD and PC-MRI could validate the predicted hemodynamic changes right after implantation of an FD.</p>

[STEPS]
?. 
?. 
?. 
?. 
?.
TITLE: KAPP-Sen TMC: Pancreas Tissue Blocks Paraffin Embedding
AUTHORS: Cristina Aguayo-Mazzucato, Kanako Iwasaki

[DESCRIPTION]
The paraffin cassettes were fixed in 4% paraformaldehyde for 24 hours and processed for paraffin embedding at Joslin Histology Core as described here.


[STEPS]
SECTION: Tissue processing using the Citadel 2000 Table Top Processor
1. Tissue is processed overnight.

 

 


SECTION: Tissue processing using the Citadel 2000 Table Top Processor
2. Tissue blocks are transferred to a vacuum paraffin bath.

SECTION: Tissue processing using the Citadel 2000 Table Top Processor
1.1. Cassettes are submersed in 50% EtOH at the end of the day.

SECTION: Tissue processing using the Citadel 2000 Table Top Processor
1.2. Processor programmed to start overnight and finish the following morning.

SECTION: Tissue processing using the Citadel 2000 Table Top Processor
3. Tissue blocks are then embedded and placed in 4°C wrapped in foil.
TITLE: Qubit dsDNA HS Assay
AUTHORS: Lakme Caceres

[DESCRIPTION]
How to use the Qubit 2.0 fluorometer to quantify dsDNA samples. It is most accurate for sample concentrations from 5 pg/uL to 120 ng/uL.

[GUIDELINES]
All reagents are stored at 4C. Allow them to equilibriate to room temperature before use:
HS Reagent (keep dark)
HS Buffer
HS Standard #1
HS Standard #2

Make sure to use the thin-wall 0.5 mL Qubit PCR tubes for all samples and standards. Only label the caps.

[STEPS]
1. To prepare the Qubit working solution, a mixture of HS Reagent and HS Buffer, take the number of samples you will be running and multiply that by 199 uL. Then add 380 for the HS Standards. Round this number up to the nearest tenth (for excess). This is the amount of HS Buffer you will need. Divide this number by 200 to get the amount of HS Reagent you need to add. 

Example: 
199(8) + 380 = 1,972 --> 1,980 uL
1,980 / 200 = 9.9 uL
Add 9.9 uL HS Reagent to 1,980 uL of HS Buffer to make enough working solution for 8                                      samples and 2 standards.
2. Add 190 uL of working solution to two Qubit tubes. Label the top of the tubes S1 and S2. Then add 10 uL of the respective HS Standard to each tube.
3. For each of your sample tubes, add 199 uL of working solution to labeled tubes. Then add 1 uL of your samples to each tube. 
4. Vortex all tubes and spin down if necessary. Incubate for 2 minutes at room temperature. 
5. On the Qubit, select dsDNA -> dsDNA High Sensitivity -> Read standards
6. Avoid touching the walls of the tube as the Qubit is very temperature sensitive. Insert standard 1 and then select Read standard. Repeat for standard 2. 
7. Select Run samples and read them one by one. Use the 1 uL sample volume. When you are done, select Data at the bottom to view all your sample concentrations and take a photo.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p><strong>Background </strong>Autochthonous cutaneous and visceral leishmaniasis caused by <em>Leishmania martiniquensis</em> and <em>Leishmania siamensis</em> have been considered an emerging infectious disease in Thailand. The disease burden is significantly underestimated, especially the prevalence of <em>Leishmania</em> infection among HIV-positive patients.</p>
<p><strong>Methods </strong>A cross-sectional study was conducted to determine the prevalence and risk factors associated with <em>Leishmania</em> infection among patients with HIV/AIDS living in Trang Province, southern Thailand between 2015 and 2016. Antibodies against <em>Leishmania</em> infection were assayed using the direct agglutination test (DAT). DNA of <em>Leishmania</em> was detected by ITS1-PCR using the buffy coat. Species of <em>Leishmania</em> were also identified.</p>
<p><strong>Results </strong>Of 724 participants, the prevalence of <em>Leishmania</em> infection was 25.1% (182/724) using either DAT and/or PCR assays. Seroprevalence of<em> Leishmania </em>infection was 18.5% (134/724), while <em>Leishmania </em>DNA detected by PCR method was 8.4% (61/724). Of these, 24.9% (180/724) were asymptomatic, whereas 0.3% (2/724) were symptomatic VL and VL/CL. At least five species were identified, that is, <em>L. siamensis</em>, <em>L. martiniquensis</em>, <em>L. donovani</em> complex, <em>L. lainsoni,</em> and <em>L. major</em>. Multivariate analysis showed that CD4+ levels &lt;500 cells/µL, and living in stilt houses were independently associated with <em>Leishmania</em> infection. Those who were PCR positive for <em>Leishmania</em> DNA were significantly associated with detectable viral load. Whereas, non-injection drug use (NIDU) and CD4+ levels &lt;500 cells/µL were potential risk factors of <em>Leishmania </em>seropositivity.</p>
<p><strong>Conclusions </strong>A magnitude of prevalence of underreporting <em>Leishmania</em> infection among Thai patients with HIV was revealed in this study. Effective public health policy to prevent and control disease transmission is urgently needed.</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
This protocol provides a method for identifing CRIPR repeats using the program PilerCR on assembled contigs. Based on the methods from the following publication: <br /><br />Hannigan, Geoffrey D., et al. "The Human Skin Double-Stranded DNA Virome: Topographical and Temporal Diversity, Genetic Enrichment, and Dynamic Associations with the Host Microbiome." <em>mBio</em> 6.5 (2015): e01578-15.

[BEFORE_START]
Perl scripts and other supplemental information available at:<br /><br /><a href="https://figshare.com/articles/The_Human_Skin_dsDNA_Virome_Topographical_and_Temporal_Diversity_Genetic_Enrichment_and_Dynamic_Associations_with_the_Host_Microbiome/1281248" target="_blank">https://figshare.com/articles/The_Human_Skin_dsDNA_Virome_Topographical_and_Temporal_Diversity_Genetic_Enrichment_and_Dynamic_Associations_with_the_Host_Microbiome/1281248</a>

[GUIDELINES]
Required Software:<br />
<ul>
<li>PilerCR v1.06</li>
<li>NCBI's BLAST+ v2.2.0</li>
<li>mothur</li>
</ul>
Relevant Files<br />Output:<br />
<ul>
<li>CRISPRs/spacer_lengths.txt</li>
<li>CRISPRs/all_viral_hits.txt</li>
<li>CRISPRs/viral_hit_seqs_blastn_formatted.txt</li>
<li>CRISPRs/read_assignment_blastn.txt</li>
<li>CRISPRs/read_spacer_assignment.txt</li>
</ul>
R Script: <a href="https://www.protocols.io/view/Script-R16-CRISPR-Analysis-ejqbcmw" target="_blank">R16</a>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Toehold switch assembly
AUTHORS: Junyan Qian

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">General toehold assemble method using NEB Q5 High-Fidelity 2x Master Mix. Here we took Boir Noir toeholds as an example, it has four candidates we wanted to test. In order to have a higher yield, run 5 reactions for each candidates.</div></div>

[STEPS]
?. [PCR preparation]
Prepare 4 effendorfs label them, each one will contain the master mix of a certain toehold primer.Attention: Keep the polymerase on ice avoiding denaturation.(Order of pipetting: H2O -> Q5 MasterMix -> primers)  ABCDEF1Number of reactions per Master mix:52Reaction Volume:2534Reference (25 uL)Master mix1Master mix2Master mix3Master mix45DNA template(~60ng/uL)16Common primer (10μM)1.256.256.256.256.257Toehold_primer_1(10μM)1.256.258Toehold_primer_2(10μM)6.259Toehold_primer_3(10μM)6.2510Toehold_primer_4(10μM)6.2511Q5 MasterMix12.562.562.562.562.512H2O (DNAse RNAse free distill)94545454513Total25120120120120
ABCDEF1Number of reactions per Master mix:52Reaction Volume:2534Reference (25 uL)Master mix1Master mix2Master mix3Master mix45DNA template(~60ng/uL)16Common primer (10μM)1.256.256.256.256.257Toehold_primer_1(10μM)1.256.258Toehold_primer_2(10μM)6.259Toehold_primer_3(10μM)6.2510Toehold_primer_4(10μM)6.2511Q5 MasterMix12.562.562.562.562.512H2O (DNAse RNAse free distill)94545454513Total25120120120120
?. [PCR preparation]
Pipette up and down mix the master mix thoroughly.
?. [PCR preparation]
Take 20 PCR tubes label them, pipette 24 μl of the master mix into the tubes accordingly.
?. [PCR preparation]
Add 1 μl  of DNA template into each PCR tubes
?. [PCR]
PCR machine setting: Calculate the reaction temperature according to the primer sequence using NEB online Tm calculator:  http://tmcalculator.neb.com/#!/main  ABCDEFG1TemplateFw PrimerRev PrimerLengthElongation timeTm - Q5Tm used2DNA templateToehold primerCommon primer900-100030sT1/T2T = Average (T1,T2)
ABCDEFG1TemplateFw PrimerRev PrimerLengthElongation timeTm - Q5Tm used2DNA templateToehold primerCommon primer900-100030sT1/T2T = Average (T1,T2)
?. [PCR]
Start the PCR reaction.
?. [PCR]
Thermo cycle setting:  ABC1Initial Denaturation98°C30 seconds225–35 Cycles98°C5–10 seconds3T°C10–30 seconds472°C20–30 seconds/kb5Final Extension72°C2 minutes6Hold4°C&nbsp;
ABC1Initial Denaturation98°C30 seconds225–35 Cycles98°C5–10 seconds3T°C10–30 seconds472°C20–30 seconds/kb5Final Extension72°C2 minutes6Hold4°C&nbsp;
TITLE: Cell lysis and immunoblotting
AUTHORS: Kelsey Hickey, Sharan Sharan Swarup, Harper JW

[DESCRIPTION]
This is cell lysis and immunoblotting protocol. 

[STEPS]
SECTION: Harvest and Lyse cells
1. Cells were cultured in the presence of the corresponding stress to 60-80% confluency in 6-well plates, 10 cm or 15 cm dishes. After removing the media, the cells were washed with DPBS three times. 

SECTION: Harvest and Lyse cells
2. To lyse cell urea buffer (8M urea, 50 mM TRIS 7.5, 150 mM NaCl, containing mammalian protease inhibitor cocktail (Sigma), Phos-STOP, and 20 unit/ml Benzonase (Millipore)) was added directly onto the cells. Cell lysates were collected by cell scrapers and sonicated on ice for 10 seconds at level 5, and lysates were cleared by centrifugation (15000 rpm, 10 min at 4 oC). 

SECTION: Run SDS-page gel and transfer to membrane for immunoblotting
3. The concentration of the supernatant was measured by BCA assay. For immunoblotting, the whole cell lysate was denatured by the addition of LDS sample buffer supplemented with 100 mM DTT, followed by boiling at 95oC for 5 minutes

SECTION: Run SDS-page gel and transfer to membrane for immunoblotting
4. 10-20 mg of each lysate was loaded onto the 4-20% Tris-Glycine gel (Thermo Fisher Scientific) or 4-12% NuPAGE Bis-Tris gel (Thermo Fisher Scientific), followed by SDS-PAGE with Tris-Glycine SDS running buffer (Thermo Fisher Scientific) or MOPS SDS running buffer (Thermo Fisher Scientific), respectively. 

SECTION: Run SDS-page gel and transfer to membrane for immunoblotting
5. For Chemiluminescence westerns: The proteins were electro-transferred to PVDF membranes (0.45 µm, Millipore), and then the total protein was stained using Ponceau (Thermo Fisher Scientific). The membrane was then blocked with 5% non-fat milk (r.t., 60 min) incubated with the indicated primary antibodies (4oC, overnight), washed three times with TBST (total 30 min), and further incubated either with HRP conjugated anti-Rabbit and anti-mouse secondaries at (1:5,000) for 1h. After thorough wash with TBST for 30 min membranes were treated with Lightning‱ Plus Chemiluminescence Reagent (PerkinElmer, NEL104001EA) after mixing the Enhanced Luminol Reagent and the Oxidizing Reagent 1:1. Mixed Chemiluminescence Reagent was added to blot and rocked gently for 1 minute and imaged using BioRad ChemiDoc Imaging System

SECTION: Run SDS-page gel and transfer to membrane for immunoblotting
6. 

For LI-COR westerns: The proteins were electro-transferred to nitrocellulose membranes and then the total protein was stained using Ponceau (Thermo Fisher Scientific). The membrane was then blocked with LI-COR blocking buffer at room temperature for 1h. Then membranes were incubated with the indicated primary antibodies (4oC, overnight), washed three times with TBST (total 30 min), and further incubated either with fluorescent IRDye 680RD Goat anti-Mouse IgG H+L, or IRDye 800CW Goat anti-Rabbit IgG H+L secondary antibody at (1:10,000) at room temperature for 1h. After thorough wash with TBST for 30 min, near infrared signal was detected using OdysseyCLx imager and quantified using ImageStudioLite (LI-COR).
TITLE: Safety Training - Rice/Correa Lab
AUTHORS: Samantha Coy

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">In order to work in the Correa Lab, new members must complete both the General Laboratory Safety Training and Blood Bourne Pathogen Safety Training offered by Rice University. They must also be trained by the Correa Lab member currently in charge of safety. Annual refreshers can be completed online. </div></div>

[STEPS]
?. Navigate to Rice University's Environmental Health Safety Training Schedule and Registration Page
?. If this is your first time receiving this training, you must attend sessions in person for 1) General Laboratory Safety Training, and 2) Biosafety and Bloodborne Pathogens Safety Training before you can work in the Correa Lab. Sign up for both sessions (they usually occur on the same day, consecutively) and attend the training. If you need training sooner than the next available courses, contact cmize@rice.edu.
?. Once you have completed the in-person or online refresher course, you should receive files containing safety certifications for each course that will need to be printed out. Both you and Dr. Correa need to sign these and place these in the Safety Certification binder.
?. If this is not your first time getting this training, you can complete the online refresher course for BOTH the General Laboratory Safety session and Biosafety and Bloodborne Pathogens Safety session. After signing up with your Rice netID credentials, the refersher will appear in your Canvas Dashboard where you can find and complete the training modules.
?. You will also need to contact whomever is in charge of safety in the Correa Lab (currently Dr. Amanda Shore, ashore@rice.edu) to schedule lab safety training. This will familarize you with where everything is in the lab (first-aid kit, eye-wash stations, etc.).