eamag/protocols-with-steps huggingface dataset:
TITLE: Experimento 2: Colores
AUTHORS: Javier Lopez, Eduardo Antonio Mancilla Pacheco, Luis Emiliano Villegas Sapiens

[DESCRIPTION]
Este protocolo detalla un experimento diseñado para investigar la influencia del color en el cambio de temperatura. Se utilizan tres botellas de diferentes colores, y las mediciones de temperatura se realizan con Arduino. El objetivo es explorar cómo las variaciones en la absorción y emisión de calor podrían afectar el comportamiento térmico de los objetos.

[STEPS]
SECTION: Experimento
1. Instalar Arduino
SECTION: Experimento
2. Programar el Arduino para la recopilación de datos
SECTION: Experimento
3. Conectar los sensores de temperatura
SECTION: Experimento
5. Calentar agua a 80ºC
SECTION: Experimento
4. Verificar que se registren las mediciones
SECTION: Experimento
6. Llenar las botellas de agua caliente
SECTION: Experimento
3.1. Colocarlos en las botellas
SECTION: Experimento
7. Tomar mediciones de temperatura
SECTION: Experimento
7.1. Hasta que el agua llegue a temperatura ambiente
SECTION: Experimento
8. Limpiar los datos
SECTION: Experimento
9. Graficar en MatLab
TITLE: wafarghsg
AUTHORS: sf  

[DESCRIPTION]
gbsfbbfgb

[STEPS]
SECTION: 21.08.2066
1.    fgsgrtvsvxfdgbhgdbbgfxbxgbdfbcvbfxbgbxbggdfgxbdfgb
SECTION: 23.98.6778
2. fgdbdgnffgnbg
SECTION: 23.98.6778
2.1.
TITLE: Cryogenic (H2O)n-GCIB-SIMS imaging 
AUTHORS: Hua  Tian

[DESCRIPTION]
The protocol describes the imaging of frozen-hydrated biological sample using high resolution mass spectrometry imaging, water gas cluster ion beam secondary ion mass spectrometry (H2O)n-GCIB-SIMS).

[STEPS]
1. Both (H2O)n-GCIB and C60-SIMS were performed on a buncher-ToF instrument, J105 3D Chemical Imager (Ionoptika, Southampton, UK. Abbv. J105). The water cluster ion beam is pulsed through a pulser in the gun column, where the distance to the sample surface is 0.533 m. Beam tuning was assisted with an oscilloscope (Tektronix TDS 2024, USA) with detection by a secondary electron detector (SED). The singly-charged (H2O)n cluster size at beam energy of 70 kV with a time of flight (ToF) of 103 µs was calculated using the ToF equation as n = 30,900 (Figure S15). The SED offset was 8 µs. Beam focus was measured by scanning a 1000 mesh grid (Agar Scientific, Essex, UK). The average beam spot sizes were calculated using 20/80 percent of maximum intensities and were 1.60±0.01 µm and 1.16±0.45 µm for 70 keV (H2O)30k+ and 40 kV C60+, respectively (Figure S14). The beam dither was adapted to match the image pixel size. The mass resolution m/Δm was 6875 around m/z 100, and 10,000~12,000 up to m/z 2000. The live readout of mass resolution was from the software, Ionoptika SIMS Mainframe during the data acquisition.

The gold coated Si wafer with the frozen-hydrated mouse/human liver tissue section was plunged into liquid nitrogen and inserted to the pre-chilled cold sample stage in J105 instrument and kept at 100 K during GCIB-SIMS imaging. This cryogenic sample handling preserved the frozen-hydrated state thus maintaining the chemical gradients in the tissue section. 

Guided by the anatomical features on the semi-serial H&E stained section, an area of interest was selected for SIMS imaging in negative ion mode using a 70 keV (H2O)30k+ beam. The acquisition was in negative ion mode with 256 × 256 pixels using a 2 × 2 tiled image mode for mouse liver tissue sections, or 768×768 pixels using a 3 × 3 tiled image mode for human liver tissue sections. Each tile covers 400 × 400 µm2 (3.1 µm per pixel) for each section. With 1 pA of beam current and 296 shots per pixel, the ion doses were 3.01×1012 ions/cm2 each tile.
TITLE: Protein expression of hard-to-produce proteins in the cytoplasm of Escherichia coli
AUTHORS: Cristina Hernandez Rollan, Kristoffer Bach Falkenberg, Maja Rennig, Andreas Birk Bertelsen, Morten Norholm

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol describes the expression and extraction of heterologously cytoplasmically expressed proteins in the bacterium </span><span style = "font-style:italic;">E.coli</span><span>, for T7 based expression systems. </span></div><div class = "text-block">Specifically, it describes how to produce "hard-to-produce", as for example, LPMOs.</div><div class = "text-block">It is based on a modified version of:</div><div class = "text-block"><span style = ":UNDERLINE;">"</span><span style = ":UNDERLINE;">IPTG Induction and  Extraction of Proteins from Bacteria" </span><span>by Swathi Arur and Sudhir Nayak, Schedl Lab. Washington University Genetics, St. Louis. </span></div><div class = "text-block">Hemsworth, G. R., Henrissat, B., Davies, G. J., and Walton, P. H. (2014) Discovery and characterization of a new family of lytic polysaccharide monooxygenases. Nat. Chem. Biol.10, 122–126.</div><div class = "text-block">The extraction of cytoplasmatic proteins is followed by a chemical lysis protocol, alternatively, sonication can also be employed.</div></div>

[STEPS]
?. [Day 1 - Culture inoculation]
Pick a fresh colony of your BL21 (DE3) strain previously transformed with your expression vector, and inoculate it in LB medium supplemented with relevant antibiotics. Grow the culture at  at 250 RPM shaking . The volume of the overnight culture depends on the volume of the expression culture and should be at least 1/100 of the expression culture
37 °C
?. [Day 2 - Induction]
Dilute the overnight culture 1:100 in fresh LB supplemented with relevant antibiotics
?. [Day 3 - Collection and lysis]
Collect induced samples by centrifugation at  and carefully discard the supernatant
Centrifuge: 8000 34, 20 min, 4 10
Pellets can be frozen here at   or   until protein extraction
?. [Day 2 - Induction]
Grow the culture at  with 250 RPM shaking until an OD600 = 0.5 - 0.6
37 °C
?. [Day 2 - Induction]
Induce the expression by adding IPTG to a final concentration of
?. [Day 2 - Induction]
Grow the culture for   at  with 180 RPM shacking.
18 °C
The temperature can be lowered further allowing for a slower induction in order to increase the production of soluble product.
?. [Day 3 - Collection and lysis]
Lyse the cells using a lysis protocol or a commercially available kit
?. [Day 3 - Collection and lysis]
Keep the extractions  when working with it and at  for storage
on ice
4 °C
?. [Day 2 - Induction]
Move the culture into an incubator set to  with 180 RPM of shaking and allow the culture to reach OD600 = 0.8 - 1.0
18 °C
TITLE: No Title
AUTHORS: 

[GUIDELINES]
<p>Glassware should be acid-washed and rinsed well with dH<sub>2</sub>O before use. If unable to measure small quantities accurately, either scale up the recipe or prepare stock solutions of the individual compounds.</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Calcium Imaging in mDA neurons
AUTHORS: gurvir.virdi

[DESCRIPTION]
Calcium imaging using Fura-2, Fluo-4 and X-Rhod-1.

[STEPS]
1. To measure cytosolic calcium, 5 uM Fura-2 or Fluo-4 was added to cells at room temperature for 40 minutes made up in HBSS (Invitrogen).

To measure mitochondrial calcium, cells were incubated with 2μM X-Rhod-1 in HBSS for 40 minutes. 
2. Cells are then washed 2x in HBSS at room temperature. They are now ready for imaging.
1.1. All probes are from Molecular Probes (Thermo Fisher Scientific). Fura-2-AM is a ratiometric dye with a high affinity for Ca2+. It is used to measure [Ca2+]c.

Fluo-4-AM is a calcium indicator.

X-Rhod-1-AM is a calcium indicator which localises to mitochondria.
3. [Ca2+]c is monitored in cells by obtaining the ratio between the excitation at 340 nm (high Ca2+) and 380 nm (low Ca2+) for which fluorescence light was reflected through a 515 nm long pass filter. 
4. Microscopy for Fluo-4:
5. Microscopy for Fura-2 [Ca2+]c:

5.1. The cells were excited sequentially at 340 and 380nm using light from a Xenon arc lamp. A time-series with 1 or 5 second intervals was performed to establish basal fluorescence before 50mM KCl was added to depolarise the membrane.The emitted fluorescence was measured at 515 nm on a cooled camera device (CCD).
5.2. [Ca2+]c  using Fura-2 AM was also imaged on a Nikon Ti2 inverted microscope with Perfect Focus System, an ASI motorised XY stage with piezo Z and an Okolab environmental chamber with a CO2 mixer. Imaged were acquired using an Andor iXon Ultra897 EMCCD camera. Cells were excited with a Cairn FuraLED light engine optimised for 340 and 380 nm with a dichroic mirror T400lp (Chroma) and an emission filter ET510/80m (Chroma), using a 40x 1.3 NA S Fluor objective. The microscope was controlled with Micro-Manager v2.0
4.1. Live-cell imaging was performed excited by a 488 nm laser and measured at 520 nm. A time-series with 5 second intervals was performed to establish basal fluorescence before 50mM KCl was added to depolarise the membrane and measure fluorescence intensity increase, and recovery. 
6. Microscopy for X-Rhod-1:
6.1. Live-cell imaging was performed excited by a 561 nm laser. A time-series with 5 second intervals was performed to establish basal fluorescence before 50mM KCl was added to depolarise the membrane and measure fluorescence intensity increase, and recovery giving readouts for mitochondrial calcium.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>We developed an Illumina-based pipeline, HIFI-Barcode, to produce full-length COI barcodes from pooled PCR amplicons generated by individual specimens. Using indexed primer sets and high-throughput sequencing platform strategy, and optimized analysis pipeline, the analytical cost and chemistry cost will significantly be reduced. The new protocol includes DNA preparation, amplification, and data analysis pipeline.</p>

[BEFORE_START]
<p>Before starting this work, we recommend you read the paper we have published, "Shanlin Liu, Chentao Yang, Chengran Zhou, Xin Zhou; Filling reference gaps via assembling DNA barcodes using high-throughput sequencing—moving toward barcoding the world, <em>GigaScience</em>, Volume 6, Issue 12, 1 December 2017, Pages 1–8, <a href="https://doi.org/10.1093/gigascience/gix104" target="_blank">https://doi.org/10.1093/gigascience/gix104</a>", and if you have any question, just contact us.</p>
<div class="grammarly-disable-indicator"> </div>

[STEPS]
?. 
?. 
?. 
?. 
?.
TITLE: Odor Threshold
AUTHORS: sdwalto, Jeffrey Kordower, Bryan_Killinger

[DESCRIPTION]
Odor threshold optimized for mice. This test was made to test short term olfactory memory. Mice with olfactory impairment or deficits should score lower. 

[GUIDELINES]
ODORANT: Propionic acid at increasing concentrations of 1:106, 1:104, and 1:103 diluted in mineral oil. 
 
Notes:
- Paper swabs soaked in the different odorant dilutions were used inside the cartridges.
- Investigation time defined as the duration of active sniffing with the nose placed less than 1 cm away from the cartridge. 
 

[STEPS]
1. Prepare and empty rodent cage with no bedding.
2. Prepare the odorant by diluting in mineral oil. (See guidelines)
3. Acclimate mouse to setup (empty cage with odor cartridges). Place mouse in the cage and expose to empty odor cartridge for 5 mins. 
4. Pre-habituate mouse to a paper swab soaked in mineral oil in the odor cartridge. (5 mins). 
5. Habituation phase. Expose mouse to mineral oil for three 50 secs trials with a 5 min interval between trials. 
6. Detection phase. Expose mouse to lowest concentration of propionic acid for 50 secs and record time spent investigating the odor cartridge. 
7. Repeat step 6 with the other concentrations of propionic acid, starting from lowest to highest.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This protocol modified from: </p>
<p>Pandey A, Bhathena Z. 2014. Prevalence of PUFA Rich Thraustochytrids sps. along the<br />Coast of Mumbai for Production of Bio Oil. Journal of Food and Nutrition<br />Research 2(12): 993-999.</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
Genomic DNA was extracted from circulating blood leucocytes by using Gentra Puregene Blood Kits according to the manufacturer’s protocol (Qiagen) and was stored at -20°C. 
The TRKB polymorphismswere genotyped by the IntegraGen company (Evry, France) using the Fluidigm®BioMarkTMHD system (Fluidigm Corporation, CA, USA), using TaqMan allelic discrimination (Coulbault et al., 2006), with the ABI Prism® 7900HT Sequence Detection Sys­tem (Life Technologies).

[STEPS]
TITLE: Generation of stable cell lines via lentiviral transduction
AUTHORS: Elias Adriaenssens

[DESCRIPTION]
Here, we describe a protocol to generate stable cell lines using a lentivirus system. Please note that necessary safety measures are to be taken in working with lentivirus.

[STEPS]
SECTION: Packaging lentiviral plasmid into a lentiviral particles for in…
1. Grow HEK293T cells to 60-70% confluency in Growth media in a 6-well Petri Dish.
SECTION: Packaging lentiviral plasmid into a lentiviral particles for in…
2. Prepare a transfection mix in a sterile 1.5 ml Eppendorf tube, containing: 
      lentiviral vector   with your gene-of-interest  1500 ng    Gag/Pol plasmid  1000 ng    VSV-G plasmid  500 ng    P3000 reagent (Lipofectamine 3000 kit)  5 µL    OptiMem  125 µL  
 
SECTION: Packaging lentiviral plasmid into a lentiviral particles for in…
3. Prepare Lipofectamine 3000 mixture in a sterile 1.5 ml Eppendorf tube, containing: 
      Lipofectamine 3000  5 µL    OptiMem  125 µL  
 
SECTION: Packaging lentiviral plasmid into a lentiviral particles for in…
4. Incubate each mixture (from steps 2 and 3) separately for ~ 5 min at Room temperature.

SECTION: Packaging lentiviral plasmid into a lentiviral particles for in…
5. Mix the two suspensions and incubate at Room temperature for 15 min.

SECTION: Packaging lentiviral plasmid into a lentiviral particles for in…
6. Add the mixture drop-wise to the cells from step 1 using a P1000 sterile pipette.
SECTION: Packaging lentiviral plasmid into a lentiviral particles for in…
7. Incubate cells at 37 °C for 1440 min.

SECTION: Packaging lentiviral plasmid into a lentiviral particles for in…
8. Collect the supernatant from the cells (that now contains the lentiviruses) and pass it through a   syringe filter. If needed, add fresh growth medium and collect this too 1440 min later.
SECTION: Lentiviral infection of HeLa cells
9. Add 2 µL of polybrene (8 mg/ml) to 2 mL of lentivirus infection media from step 8 to HeLa cells seeded in 6-well plate (at 700.000/well) at 60% confluence.

SECTION: Lentiviral infection of HeLa cells
10. Incubate at 37 °C for 1440 min.

SECTION: Lentiviral infection of HeLa cells
11. Change media to fresh medium and incubate until confluency. Split three times before cells can leave the viral S2 lab.
SECTION: Lentiviral infection of HeLa cells
12. Cells can now be passaged and plated for experiments or frozen down for long-term storage in liquid nitrogen.
TITLE: Preparation of LRRK2 RCKW cryo-EM grids
AUTHORS: Mariusz Matyszewski

[DESCRIPTION]
This is Leschziner's Lab updated protocol for making cryo-EM grids for LRRK2 RCKW. This protocol, when using lower protein concentration, results in better monomer and dimer formation than the old protocol.

[BEFORE_START]
Decide which protein concentration to use, and create the proper LRRK2 buffers in order to obtain the right salt concentration (80 mM NaCl).

[STEPS]
SECTION: Freezing Grids
2. Plasma clean grids.
We used UltrAuFoil Holey Gold 1.2/1.3 300 mesh grids and plasma cleaned them in the Solarus II (Gatan) using the QuantiFoil Au preset.
SECTION: Freezing Grids
4. Apply protein to grids and plunge freeze.
We used a Vitrobot (FEI) to blot away excess sample and plunge freeze
SECTION: Freezing Grids
5. Store grids in liquid nitrogen until ready for imaging.
SECTION: Preparing Sample
1. Spin down purified LRRK2 RCKW. 10000 rcf, 10 min, 4 °C 
Leave protein on ice afterwards.

 

SECTION: Freezing Grids
3. Dilute samples to desired concentration in the LRRK2 buffer. Make sure final salt is at 80 mM NaCl.
For best results, make 10 µL samples, good for freezing 2 grids. This is to minimize time spent outside of storage buffer, reducing aggregation.
 

SECTION: Freezing Grids
3.1. (Optional) If using inhibitors, let them incubate on ice in the final LRRK2 buffer before plunge freezing.
TITLE: Crystallisation protocol for SARS-CoV-2 nsp3 macrodomain in P43
AUTHORS: Jasmin Aschenbrenner, Peter Marples, Charlie Tomlinson, Lizbé Koekemoer, Daren Fearon

[DESCRIPTION]
The COVID-19 pandemic has demonstrated the need for novel therapeutic interventions and improved pandemic preparedness strategies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This protocol details an optimized crystallization method for the SARS-CoV-2 nsp3 macrodomain, a potential drug target. Using sitting drop vapor diffusion, we describe specific buffer conditions and procedures to consistently produce high-quality crystals suitable for XChem fragment screening. The method yields crystals that diffract to an average resolution of 1.2 Å, enabling high-resolution structural studies.
All structures solved during the development of tool compounds for the SARS-CoV-2 nsp3 macrodomain are deposited on the PDB (Group deposition: G_1002283).

[STEPS]
SECTION: Crystallization experiment
3. Protein and buffer requirements:
45 µL47 mg/mL  
2.0 mL 

SECTION: Crystallization experiment
5. Dispense 20 µL into SwissCI 3 lens plate reservoir wells using a 100 µl multi-channel pipette.
Dispense 150 nL47 mg/mL    to each lens using the SPT mosquito.
Dispense  150 nL to each lens using the SPT mosquito.

Drop ratio: 1:1 ratio (150 nL   : 150 nL reservoir solution)
Final drop volume: 300 nl
SECTION: Crystallization experiment
4. Crystallisation screen composition:
100 millimolar (mM) CHES, pH 9.5
30% w/v PEG 3000

Stock solutions used:
1 Molarity (M) CHES adjusted to pH 9.5 with NaOH
50% w/v PEG 3000

 

SECTION: Crystallization experiment
7. Crystal form after ~24 h.

 


SECTION: Crystallization experiment
6. Incubate at 20 °C for 2880 min in Formulatrix Rock Imager.

Imaging Schedule: The first images are taken after 12 h and the imaging schedule follows a Fibonacci sequence of days for further collections. 
SECTION: Equipment needed
2. Formulatrix Rock Imager (or incubator of choice)
SPT mosquito
P100 multi-channel pipette
SwissCI 3 lens plate

SECTION: SARS-CoV-2 nsp3 macrodomain expression and purification
1. The protein usedfor crystallisation was expressed and purified using the following protocol.
 

SECTION: Data Collection at Synchrotron
8. Diamond Light Source
Unattended Data Collection (UDC)
Data Collection Temperature: 100K
Detector: DECTRIS EIGER2 X 9M
Beamline: I04-1
Wavelength:  0.9212 Å
Resolution (Å): 1.62
Beam Size (μm): 60 X 50
Number of images: 3600
Oscillation: 0.10°
Exposure (s): 0.0020
Transmission (%): 100
Flux (ph/s): 3.80e+12
TITLE: Determination of flavonoid content
AUTHORS: Jorge Carlos Ruiz Ruiz

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Flavonoid content determination using the aluminum chloride method.</div></div>

[STEPS]
?. An aliquot of sample (0.1 mL) was mixed with 0.4 ml distilled water in a 1.5 mL microcentrifuge tube.
?. 0.03 mL of 5% NaNO2 was added and the mixture was allowed to react for 5 min.
?. Following this, 0.03 mL of 10% AlCl3 was added and the mixture stood for a further 5 min.
?. Finally, the reaction mixture was treated with 0.2 mL of 1 M Na2CO3 and 0.24 mL distilled water, and the absorbance at 510 nm.
?. Catechin (Sigma-Aldrich) was used as the standard and the results were expressed as µg/mL of catechin equivalents (CE).
TITLE: RNA/DNA extraction from samples of acute gastroenteritis
AUTHORS: Hengyun Guan, Chunrong Wang, Lanzheng Liu, Guoliang Yang

[DESCRIPTION]
<p>Many types of sample would be collected in outbreaks of acute gastroenteritis. Therefore we have settled this protocol to extract RNA/DNA for virus detection rapidly and effectively.</p>

[STEPS]
?. [Pretreatment of stool/anal swab]
Add 0.2g stool to 1.0ml phosphate buffer saline (with Mg2+ and Ca2+) in one 1.5ml Eppendorf tube with 6-8 particles of ceramic beads. Put the anal swab into 1.0ml phosphate buffer saline (with Mg2+ and Ca2+) and repeatedly squeeze it.
 Vortex for 2×20 sec at 4000rpm at room temperature.Centrifuge at 8000 rpm for 10 min at 4℃.Collect the supernatant.
?. [Preparing swabs]
5. Throat swabs or environmental surface swabs were stored in 3.5ml Hank’s solution （Yocon Catalog #:MT0301-1）.6. Vortex for 40 sec at 4000rpm at room temperature.
?. [Pretreatment of water]
7. Add 15ml contaminated water to centrifugal filter (Merck Millipore Ltd., Ireland).8. Centrifuge at 8000rpm for 5min at 4℃.9. Repeat step 8 for three times.10. Collect the supernatant.
?. [RNA/DNA extraction]
16. Add each above 200ul supernatant in sample cartridge to extract RNA/DNA followed the manufacturer’s instructions (MagNA Pure LC Total Nucleic Acid Isolation Kit, Roche, Germany).17. RNA/DNA was suspended in 50ul of elution buffer.18. RNA/DNA was amplified immediately or stored at −80℃.
?. [Pretreatment of food:]
Select randomly six points on the surface of food specimen, then cut them into 2mm×2mm×2mm pieces by sterilized scissors.Add 0.2g food pieces to 1.0ml phosphate buffer saline (with Mg2+ and Ca2+) in one 1.5ml Eppendorf tube with 6-8 particles of ceramic beads. Vortex for 2×20 sec at 4000rpm at room temperature.
  Centrifuge at 8000 rpm for 10 min at 4℃.
  Collect the supernatant.
?. [Note:]
The left supernatant was stored at −80℃ after the RNA/DNA extraction.
TITLE: ISP French Polynesia Recreational Scuba Diving
AUTHORS: Stephanie Gay

[DESCRIPTION]
This landscape study aims to assess the current state of the recreational diving industry in French Polynesia, particularly from a local community perspective. The main focus of study will be to investigate local perceptions of industry. Particularly:
The perceived impact on the local community – whether positive or negative
The perceived presence or absence of local participation – motivators and deterrents such as existing barriers to entry in the industry

To achieve the aims outlined, this study will also need to investigate the wider context of the French Polynesian recreational dive industry – information about which seems to be lacking in current literature. Hence, in the following protocol I highlight the procedure I followed to gather some preliminary information about the demographics of recreational scuba dive clubs in French Polynesia

[STEPS]
SECTION: Background Web Research on Dive Clubs in French Polynesia
1. Navigate to Google Directory of dive clubs in French Polynesia through query "Scuba diving centres in French Polynesia"
SECTION: Background Web Research on Dive Clubs in French Polynesia
2. Navigate to https://www.tahititourisme.com/activities/diving/diving-clubs/
SECTION: Background Web Research on Dive Clubs in French Polynesia
1.1. 
 
Click on "More Places"
SECTION: Background Web Research on Dive Clubs in French Polynesia
1.2. 
 
Open "Website" link for each directory entry in a new tab
SECTION: Background Web Research on Dive Clubs in French Polynesia
2.1. 
 
Open in a new tab each entry listing a club whose club website was not opened in step 1
SECTION: Background Web Research on Dive Clubs in French Polynesia
2.2. 
 
For each page, open in a new tab the club's website 
5. Scroll or navigate to the portion of the webpage that lists the club's staff - typically listed under "Our Team"
 

3. On each dive club's website, select the language choice to be English or use web browser built-in French to English translation tools


 

 

4. Note down the club name, location and web address in a spreadsheet

 
 
6. Note down the number of instructors and types of certifications possessed in the same spreadsheet. If no certification info is listed, also note this down.
7. Repeat for each dive club