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<p><strong>A-2. <u>ISCO Sampling Protocol</u></strong></p>
<p>River and estuary samples will be collected using an automated ISCO sampler using the following protocol:</p>
<p> </p>
<ul>
<li>Sampler Description:</li>
</ul>
<ul>
<li>A Teledyne ISCO 3700 full size portable sequential sampler is used for sampling.</li>
<li>ISCO sampler consists of a rosette of 24, one-liter polypropylene bottles in which samples are collected at regular intervals.</li>
<li>Includes a pump, which pumps water through a ¼-inch, flexible sample line into sample bottles. Sample line is automatically rinsed before each new sample is collected.</li>
<li>Controller on ISCO sampler allows for sample programming.</li>
</ul>
<p> </p>
<ul>
<li>Sampler Installation:</li>
</ul>
<ul>
<li>The ISCO samplers are installed within a sturdy, locked enclosure that is protected from damage due to tampering and environmental hazards (usually installed within a U.S. Geological Survey gaging station).</li>
<li>ISCO samplers are installed by extending the ¼-inch suction line from the sampler into the water body being sampled.</li>
<li>Sample line is secured using PVC pipe attached to a permanent structure, such as a river wall or concrete river bed.</li>
<li>A Campbell<sup>®</sup> Scientific CR1000 datalogger and AirLink<sup>®</sup> LS300 Raven cell modem is installed to communicate with the ISCO, allowing for remote control and monitoring of the sampler.</li>
<li>ISCO samplers are generally programmed to collect 250 mL every 15 minutes for up to 24 hours (each bottle represents a composite, one-hour sample).</li>
</ul>
<p> </p>
<ul>
<li>Field Collection:</li>
</ul>
<ul>
<li>ISCO samplers are turned on remotely using the Campbell<sup>®</sup> Scientific LoggerLink mobile application, which communicates with the datalogger.</li>
<li>Sampler is filled with ice to ensure sample preservation and ice is replaced once per day while the sampler is running.
<ul>
<li>When samples are collected, if ice is melted, the temperature of the melted ice water is recorded on a field sheet.</li>
</ul>
</li>
<li>Water samples are collected in two-liter plastic Nalgene bottles, which are sanitized with dish soap after each use.
<ul>
<li>A small quantity of sample water from each sample bottle is poured into a Nalgene bottle, capped, shaken, and dumped to remove any soap residue from the bottle before the sample is collected (x3).</li>
<li>During and within 24 hours of an event, two ISCO sample bottles are combined into one, two-liter Nalgene bottle when the samples are collected (results in a two hour composite sample).</li>
<li>24 hours after an event, four ISCO sample bottles are combined into one, two-liter Nalgene bottle when the samples are collected (results in a four hour composite sample).</li>
<li>Samples are immediately placed on ice and taken back to the lab for filtering.</li>
</ul>
</li>
<li>One-liter ISCO sample bottles are rinsed with deionized water (x3) and replaced in the sampler after each sample is removed.</li>
<li>Bottle blanks are collected once per event at each site by collecting a sample of deionized water using the same procedure that a field sample would be collected.</li>
</ul>

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