TITLE: No Title
AUTHORS: 

[GUIDELINES]
<p>1)      10-40% linear sucrose gradient for SW27 (or SW28) rotor tubes for 6 gradients</p>
<p> </p>
<p>                    96.0 mL of sucrose (600 mg/mL)</p>
<p>                  <u>  48.0</u> mL of 3X buffer</p>
<p>                  144.0 mL total of 400 mg/mL sucrose</p>
<p>                                 add 9.0 mL to each of 6 gradients (90.0 mL remaining)</p>
<p> </p>
<p>                    90.0 mL of sucrose (400 mg/mL)</p>
<p>                  <u>  30.0</u> mL of 1X buffer</p>
<p>                  120.0 mL total of 300 mg/mL sucrose</p>
<p>                                 add 9.0 mL to each of 6 gradients (66.0 mL remaining)</p>
<p> </p>
<p>                    66.0 mL of sucrose (300 mg/mL)</p>
<p>                    <u>33.0</u> mL of 1X buffer</p>
<p>                    99.0 mL total of 200 mg/mL sucrose</p>
<p>                                 add 9.0 mL to each of 6 gradients (45.0 mL remaining)</p>
<p> </p>
<p>                    45.0 mL of sucrose (200 mg/mL)</p>
<p>                    <u>45.0</u> mL of 1X buffer</p>
<p>                    90.0 mL total of 100 mg/mL sucrose</p>
<p>                                 add 6.0 mL to each of 6 gradients (discard the remainder)<br /><br /></p>
<p>2)      20-50% linear sucrose gradient for SW27 (or SW28) rotor tubes for six gradients<br /><br /></p>
<p>                  120.0 mL of sucrose (600 mg/mL)</p>
<p>                  <u>  24.0</u> mL of 6X buffer</p>
<p>                  144.0 mL total of 500 mg/mL sucrose</p>
<p>                                 add 9.0 mL to each of 6 gradients (90.0 mL remaining)<br /><br /></p>
<p>                    90.0 mL of sucrose (500 mg/mL)</p>
<p>                  <u>  22.8</u> mL of 1X buffer</p>
<p>                  112.8 mL total of 400 mg/mL sucrose</p>
<p>                                 add 9.0 mL to each of 6 gradients (58.8 mL remaining)<br /><br /></p>
<p>                    58.8 mL of sucrose (400 mg/mL)</p>
<p>                    <u>19.8</u> mL of 1X buffer</p>
<p>                    78.6 mL total of 300 mg/mL sucrose</p>
<p>                                 add 9.0 mL to each of 6 gradients (24.6 mL remaining)<br /><br /></p>
<p>                    24.6 mL of sucrose (300 mg/mL)</p>
<p>                    <u>12.6</u> mL of 1X buffer</p>
<p>                    37.2 mL total of 200 mg/mL sucrose</p>
<p>                                 add 6.0 mL to each of 6 gradients (discard the remainder)<br /><br /></p>
<p>3)      20-40% linear sucrose gradient for SW27 (or SW28) rotor tubes for 6 gradients</p>
<p> </p>
<p>                    96.0 mL of sucrose (600 mg/mL)</p>
<p>                  <u>  48.0</u> mL of 3X buffer</p>
<p>                  144.0 mL total of 400 mg/mL sucrose</p>
<p>                                 add 10.0 mL to each of 6 gradients (84.0 mL remaining)</p>
<p> </p>
<p>                    84.0 mL of sucrose (400 mg/mL)</p>
<p>                  <u>  28.0</u> mL of 1X buffer</p>
<p>                  112.0 mL total of 300 mg/mL sucrose</p>
<p>                                 add 10.0 mL to each of 6 gradients (52.0 mL remaining)</p>
<p> </p>
<p>                    52.0 mL of sucrose (300 mg/mL)</p>
<p>                    <u>26.0</u> mL of 1X buffer</p>
<p>                    78.0 mL total of 200 mg/mL sucrose</p>
<p>                                 add 10.0 mL to each of 6 gradients (discard the remainder)<br /><br /></p>
<p>4)      5-20% linear sucrose gradient for SW27 (or SW28) rotor tubes for 6 gradients</p>
<p> </p>
<p>                    48.0 mL of sucrose (600 mg/mL)</p>
<p>                  <u>  96.0</u> mL of 1.5X buffer</p>
<p>                  144.0 mL total of 200 mg/mL sucrose</p>
<p>                                 add 9.0 mL to each of 6 gradients (90.0 mL remaining)</p>
<p> </p>
<p>                    90.0 mL of sucrose (200 mg/mL)</p>
<p>                  <u>  30.0</u> mL of 1X buffer</p>
<p>                  120.0 mL total of 150 mg/mL sucrose</p>
<p>                                 add 9.0 mL to each of 6 gradients (66.0 mL remaining)</p>
<p> </p>
<p>                    66.0 mL of sucrose (150 mg/mL)</p>
<p>                    <u>33.0</u> mL of 1X buffer</p>
<p>                    99.0 mL total of 100 mg/mL sucrose</p>
<p>                                 add 9.0 mL to each of 6 gradients (45.0 mL remaining)</p>
<p> </p>
<p>                    45.0 mL of sucrose (100 mg/mL)</p>
<p>                    <u>45.0</u> mL of 1X buffer</p>
<p>                    90.0 mL total of 50 mg/ml sucrose</p>
<p>                                 add 6.0 mL to each of 6 gradients (discard the remainder)<br /><br /></p>
<p>5)      10-40% linear sucrose gradient for SW41 rotor tubes for 6 gradients</p>
<p> </p>
<p>                    36.0 mL of sucrose (600 mg/mL)</p>
<p>                    <u>18.0</u> mL of 3X buffer</p>
<p>                    54.0 mL total of 400 mg/mL sucrose</p>
<p>                                 add 3.5 mL to each of 6 gradients (33.0 mL remaining)</p>
<p> </p>
<p>                    33.0 mL of sucrose (400 mg/mL)</p>
<p>                    <u>11.0</u> mL of 1X buffer</p>
<p>                    44.0 mL total of 300 mg/mL sucrose</p>
<p>                                 add 3.5 mL to each of 6 gradients (23.0 mL remaining)</p>
<p> </p>
<p>                    23.0 mL of sucrose (300 mg/mL)</p>
<p>                    <u>11.5</u> mL of 1X buffer</p>
<p>                    34.5 mL total of 200 mg/mL sucrose</p>
<p>                                 add 3.5 mL to each of 6 gradients (13.5 mL remaining)</p>
<p> </p>
<p>                    13.5 mL of sucrose (200 mg/mL)</p>
<p>                    <u>13.5</u> mL of 1X buffer</p>
<p>                    27.0 mL total of 100 mg/mL sucrose</p>
<p>                                 add 1.5 mL to each of 6 gradients (discard the remainder)</p>
<p> </p>
<p>6)      15-30% linear sucrose gradint for SW41 rotor tubes for 6 gradients</p>
<p> </p>
<p>                    36.0 mL of sucrose (600 mg/mL)</p>
<p>                    <u>36.0</u> mL of 2X buffer</p>
<p>                    72.0 mL total of 300 mg/mL sucrose</p>
<p>                                 add 3.5 mL to each of 6 gradients (51.0 mL remaining)</p>
<p> </p>
<p>                    51.0 mL of sucrose (300 mg/mL)</p>
<p>                    <u>10.2</u> mL of 1X buffer</p>
<p>                    61.2 mL total of 250 mg/mL sucrose</p>
<p>                                 add 3.5 mL to each of 6 gradients (40.0 mL remaining)</p>
<p> </p>
<p>                    40.0 mL of sucrose (250 mg/mL)</p>
<p>                    <u>10.0</u> mL of 1X buffer</p>
<p>                    50.0 mL total of 200 mg/mL sucrose</p>
<p>                                 add 3.5 mL to each of 6 gradients (29.0 mL remaining)</p>
<p> </p>
<p>                    29.0 mL of sucrose (200 mg/mL)</p>
<p>                    <u>10.0</u> mL of 1X buffer</p>
<p>                    39.0 mL total of 150 mg/mL sucrose</p>
<p>                                 add 1.5 mL to each of 6 gradients (discard the remainder)</p>
<p> </p>
<p>7)      5-20% linear sucrose gradient for SW41 rotor tubes for 6 gradients</p>
<p> </p>
<p>                    18.0 mL of sucrose (600 mg/mL)</p>
<p>                    <u>36.0</u> mL of 1.5X buffer</p>
<p>                    54.0 mL total of 200 mg/mL sucrose</p>
<p>                                 add 3.5 mL to each of 6 gradients (33.0 mL remaining)</p>
<p> </p>
<p>                    33.0 mL of sucrose (200 mg/mL)</p>
<p>                    <u>11.0</u> mL of 1X buffer</p>
<p>                    44.0 mL total of 150 mg/mL sucrose</p>
<p>                                 add 3.5 mL to each of 6 gradients (23.0 mL remaining)</p>
<p> </p>
<p>                    23.0 mL of sucrose (150 mg/mL)</p>
<p>                    <u>11.5</u> mL of 1X buffer</p>
<p>                    34.5 mL total of 100 mg/mL sucrose</p>
<p>                                 add 3.5 mL to each of 6 gradients (13.5 mL remaining)</p>
<p> </p>
<p>                    13.5 mL of sucrose (100 mg/mL)</p>
<p>                    <u>13.5</u> mL of 1X buffer</p>
<p>                    27.0 mL total of 50 mg/mL sucrose</p>
<p>                                 add 1.5 mL to each of 6 gradients (discard the remainder)</p>
<p> </p>
<p>8)      5-20% linear sucrose gradient for SW50.1 tubes  for 6 gradients</p>
<p> </p>
<p>                      8.0 mL of sucrose (600 mg/mL)</p>
<p>                    <u>16.0</u> mL of 1.5X buffer</p>
<p>                    24.0 mL total of 200 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (16.8 mL remaining)</p>
<p> </p>
<p>                    16.8 mL of sucrose (200 mg/mL)</p>
<p>                    <u>  5.6</u> mL of 1X buffer</p>
<p>                    22.4 mL total of 150 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (15.2 mL remaining)</p>
<p> </p>
<p>                    15.2 mL of sucrose (150 mg/mL)</p>
<p>                    <u>  7.6</u> mL of 1X buffer</p>
<p>                    22.8 mL total of 100 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (15.6 mL remaining)</p>
<p> </p>
<p>                    15.6 mL of sucrose (100 mg/mL)</p>
<p>                    <u>15.6</u> mL of 1X buffer</p>
<p>                    31.2 mL total of 50 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (discard the remainder)<br /><br /></p>
<p>9)      10-40% linear sucrose gradient for SW50.1 rotor tubes for 6 gradients</p>
<p> </p>
<p>                    16.0 mL of sucrose (600 mg/mL)</p>
<p>                    <u>  8.0</u> mL of 3X buffer</p>
<p>                    24.0 mL total of 400 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (16.8 mL remaining)</p>
<p> </p>
<p>                    16.8 mL of sucrose (400 mg/mL)</p>
<p>                    <u>  5.6</u> mL of 1X buffer</p>
<p>                    22.4 mL total of 300 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (15.2 mL remaining)</p>
<p> </p>
<p>                    15.2 mL of sucrose (300 mg/mL)</p>
<p>                    <u>  7.6</u> mL of 1X buffer</p>
<p>                    22.8 mL total of 200 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (15.6 mL remaining)</p>
<p> </p>
<p>                    15.6 mL of sucrose (200 mg/mL)</p>
<p>                    <u>15.6</u> mL of 1X buffer</p>
<p>                    31.2 mL total of 100 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (discard the remainder)</p>
<p> </p>
<p>10)    10-40% linear sucrose gradient for SW60 rotor tubes for 6 gradients</p>
<p> </p>
<p>                    12.0 mL of sucrose (600 mg/mL)</p>
<p>                    <u>  6.0</u> mL of 3X buffer</p>
<p>                    18.0 mL total of 400 mg/mL sucrose</p>
<p>                                 add 1.0 mL to each of 6 gradients (12.0 mL remaining)</p>
<p> </p>
<p>                    12.0 mL of sucrose (400 mg/mL)</p>
<p>                    <u>  4.0</u> mL of 1X buffer</p>
<p>                    16.0 mL total of 300 mg/mL sucrose</p>
<p>                                 add 1.0 mL to each of 6 gradients (10.0 mL remaining)</p>
<p> </p>
<p>                    10.0 mL of sucrose (300 mg/mL)</p>
<p>                    <u>  5.0</u> mL of 1X buffer</p>
<p>                    15.0 mL total of 200 mg/mL sucrose</p>
<p>                                 add 1.0 mL to each of 6 gradients (9.0 mL remaining)</p>
<p> </p>
<p>                      9.0 mL of sucrose (200 mg/mL0</p>
<p>                    <u>  9.0</u> mL of 1X buffer</p>
<p>                    18.0 mL total of 100 mg/mL sucrose</p>
<p>                                 add 1.0 mL to each of 6 gradients (discard the remainder)</p>
<p> </p>
<p>11)    5-25% linear sucrose gradient for SW41 rotor tubes for 6 gradients</p>
<p> </p>
<p>                    25.0 mL of sucrose (600 mg/mL)</p>
<p>                    <u>  5.0</u> mL of d-H<sub>2</sub>O</p>
<p>                    30.0 mL total of sucrose (500 mg/mL)</p>
<p> </p>
<p>                    23.8 mL of sucrose (500 mg/mL)</p>
<p>                    <u>23.8</u> mL of 2X buffer</p>
<p>                    47.6 mL total of 250 mg/mL sucrose</p>
<p>                                 add 2.4 mL to each of 6 gradients (33.2 mL remaining)</p>
<p> </p>
<p>                    33.2 mL of sucrose (250 mg/mL)</p>
<p>                    <u>  8.3</u> mL of 1X buffer</p>
<p>                    41.5 mL total of 200 mg/mL sucrose</p>
<p>                                 add 2.4 mL to each of 6 gradients (27.1 mL remaining)</p>
<p> </p>
<p>                    27.1 mL of sucrose (200 mg/mL)</p>
<p>                    <u>  9.0</u> mL of 1X buffer</p>
<p>                    36.1 mL total of 150 mg/mL sucrose</p>
<p>                                 add 2.4 mL to each of 6 gradients (21.7 mL remaining)</p>
<p> </p>
<p>                    21.7 mL of sucrose (150 mg/mL)</p>
<p>                    <u>10.8</u> mL of 1X buffer</p>
<p>                    32.5 mL total of 100 mg/mL sucrose</p>
<p>                                 add 2.4 mL to each of 6 gradients (18.1 mL remaining)</p>
<p> </p>
<p>                    18.1 mL of sucrose (100 mg/mL)</p>
<p>                    <u>18.1</u> mL of 1X buffer</p>
<p>                    36.2 mL total of 50 mg/mL sucrose</p>
<p>                                 add 2.0 mL to each of 6 gradients (discard the remainder)<br /><br /></p>
<p>12)    5-25% linear sucrose gradient for SW50.1 rotor tubes for 6 gradients</p>
<p> </p>
<p>                    10.0 mL of sucrose (600 mg/mL)</p>
<p>                    <u>  2.0</u> mL of d-H<sub>2</sub>O</p>
<p>                    12.0 mL total of 500 mg/mL sucrose</p>
<p> </p>
<p>                    11.0 mL of sucrose (500 mg/mL)</p>
<p>                    <u>11.0</u> mL of 2X buffer</p>
<p>                    22.0 mL total of 250 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (14.8 mL remaining)</p>
<p> </p>
<p>                    14.8 mL of sucrose (250 mg/mL)</p>
<p>                    <u>  4.7</u> mL of 1X buffer</p>
<p>                    19.5 mL total of 190 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (12.3 mL remaining)</p>
<p> </p>
<p>                    12.3 mL of sucrose (190 mg/mL)</p>
<p>                    <u>  7.2</u> mL of 1X buffer</p>
<p>                    19.5 mL total of 120 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (12.3 mL remaining)</p>
<p> </p>
<p>                    12.3 mL of sucrose (120 mg/mL)</p>
<p>                    <u>17.2</u> mL of 1X buffer</p>
<p>                    29.5 mL total of 50 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (discard the remainder)</p>
<p> </p>
<p>13)    5-25% linear sucrose gradient for SW60 tubes for 6 gradients</p>
<p> </p>
<p>                    10.0 mL of sucrose (600 mg/mL)</p>
<p>                    <u>  2.0</u> mL of d-H<sub>2</sub>O</p>
<p>                    12.0 mL total of 500 mg/mL sucrose</p>
<p> </p>
<p>                    11.0 mL of sucrose (500 mg/mL)</p>
<p>                    <u>11.0</u> mL of 2X buffer</p>
<p>                    22.0 mL total of 250 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (14.8 mL remaining)</p>
<p> </p>
<p>                    14.8 mL of sucrose (250 mg/mL)</p>
<p>                    <u>  4.7</u> mL of 1X buffer</p>
<p>                    19.5 mL total of 190 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (12.3 mL remaining)</p>
<p> </p>
<p>                    12.3 mL of sucrose (190 mg/mL)</p>
<p>                    <u>  7.2</u> mL of 1X buffer</p>
<p>                    19.5 mL total of 120 mg/mL sucrose</p>
<p>                                 add 1.2 mL to each of 6 gradients (12.3 mL remaining)</p>
<p> </p>
<p>                    12.3 mL of sucrose (120 mg/mL)</p>
<p>                    <u>17.2</u> mL of 1X buffer</p>
<p>                    29.5 mL total of 50 mg/mL sucrose              </p>
<p>                                 add 1.0 mL to each of 6 gradients (discard the remainder)<br /><br /></p>
<p>14)    Linear log sucrose gradient for SW27 (or SW28) rotor tubes, 14°C, Pp=1.30 for 6 gradients</p>
<p> </p>
<table>
<tbody>
<tr>
<td><strong>Tube</strong></td>
<td style="text-align: center;"><strong>Sucrose</strong></td>
<td style="text-align: center;"><strong>Layer</strong></td>
<td style="text-align: center;"><strong>Sucrose (600 mg/ml)</strong></td>
<td style="text-align: center;"><strong>2X buffer</strong></td>
<td style="text-align: center;"><strong> d-H<sub>2</sub>O</strong></td>
<td style="text-align: center;"><strong>Total</strong></td>
</tr>
<tr>
<td style="text-align: center;"> </td>
<td style="text-align: center;"><em>Conc.</em></td>
<td style="text-align: center;"><em>(mL)</em></td>
<td style="text-align: center;"><em> (mL)</em></td>
<td style="text-align: center;"><em>(mL)  </em></td>
<td style="text-align: center;"><em>(mL)  </em></td>
<td style="text-align: center;"><em>Volume </em></td>
</tr>
<tr>
<td style="text-align: center;">1 </td>
<td style="text-align: center;">334 mg</td>
<td style="text-align: center;">3.0</td>
<td style="text-align: center;">12.23</td>
<td style="text-align: center;">9.77</td>
<td style="text-align: center;">0.0</td>
<td style="text-align: center;">22.0</td>
</tr>
<tr>
<td style="text-align: center;">2   </td>
<td style="text-align: center;">304 mg</td>
<td style="text-align: center;">8.4</td>
<td style="text-align: center;">27.85</td>
<td style="text-align: center;">27.15</td>
<td style="text-align: center;">0.0</td>
<td style="text-align: center;">55.0</td>
</tr>
<tr>
<td style="text-align: center;">3  </td>
<td style="text-align: center;">270 mg</td>
<td style="text-align: center;">7.8</td>
<td style="text-align: center;">22.50</td>
<td style="text-align: center;">22.50</td>
<td style="text-align: center;">2.5</td>
<td style="text-align: center;">50.0</td>
</tr>
<tr>
<td style="text-align: center;">4  </td>
<td style="text-align: center;">230 mg</td>
<td style="text-align: center;">7.0</td>
<td style="text-align: center;">17.25</td>
<td style="text-align: center;">18.00</td>
<td style="text-align: center;">5.0</td>
<td style="text-align: center;">45.0</td>
</tr>
<tr>
<td style="text-align: center;">5  </td>
<td style="text-align: center;">184 mg </td>
<td style="text-align: center;">5.5</td>
<td style="text-align: center;">11.04</td>
<td style="text-align: center;">12.00</td>
<td style="text-align: center;">7.0</td>
<td style="text-align: center;">36.0</td>
</tr>
<tr>
<td style="text-align: center;">6    </td>
<td style="text-align: center;">120 mg</td>
<td style="text-align: center;">3.5</td>
<td style="text-align: center;">4.80</td>
<td style="text-align: center;">12.00</td>
<td style="text-align: center;">7.2</td>
<td style="text-align: center;">24.0</td>
</tr>
<tr>
<td style="text-align: center;">7   </td>
<td style="text-align: center;">0 mg</td>
<td style="text-align: center;">3.0</td>
<td style="text-align: center;">0.00</td>
<td style="text-align: center;">11.00</td>
<td style="text-align: center;">11.0</td>
<td style="text-align: center;">22.0</td>
</tr>
<tr>
<td style="text-align: center;"> </td>
<td style="text-align: center;"> </td>
<td style="text-align: center;">38.2</td>
<td style="text-align: center;"> </td>
<td style="text-align: center;"> </td>
<td style="text-align: center;"> </td>
<td style="text-align: center;"> </td>
</tr>
</tbody>
</table>
<p>                </p>
<p>15)   Linear log sucrose gradient for SW41 rotor tubes, 14°C, Pp=1.50 for 6 gradients</p>
<p> </p>
<table>
<tbody>
<tr style="height: 24px;">
<td style="text-align: center; width: 31px; height: 24px;"><strong> Tube</strong></td>
<td style="text-align: center; width: 35px; height: 24px;"><strong>Layer</strong></td>
<td style="text-align: center; width: 135px; height: 24px;"><strong>Sucrose (600 mg/ml)</strong></td>
<td style="text-align: center; width: 58px; height: 24px;"><strong>4X buffer</strong></td>
<td style="text-align: center; width: 58px; height: 24px;"><strong>2X buffer</strong></td>
<td style="text-align: center; width: 39px; height: 24px;"><strong>d-H<sub>2</sub>O</strong></td>
<td style="text-align: center; width: 42px; height: 24px;"><strong>Total</strong></td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;"> </td>
<td style="text-align: center; width: 35px; height: 20px;"><em>(mL)</em></td>
<td style="text-align: center; width: 135px; height: 20px;"><em> (mL)</em></td>
<td style="text-align: center; width: 58px; height: 20px;"><em>(mL)</em></td>
<td style="text-align: center; width: 58px; height: 20px;"><em>(mL)</em></td>
<td style="text-align: center; width: 39px; height: 20px;"><em>(mL)</em></td>
<td style="text-align: center; width: 42px; height: 20px;"><em>Volume</em></td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">1</td>
<td style="text-align: center; width: 35px; height: 20px;">1.8</td>
<td style="text-align: center; width: 135px; height: 20px;">6.50</td>
<td style="text-align: center; width: 58px; height: 20px;">3.0</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 39px; height: 20px;">2.50</td>
<td style="text-align: center; width: 42px; height: 20px;">12.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">2</td>
<td style="text-align: center; width: 35px; height: 20px;">3.3</td>
<td style="text-align: center; width: 135px; height: 20px;">10.80</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">12.0</td>
<td style="text-align: center; width: 39px; height: 20px;">1.20</td>
<td style="text-align: center; width: 42px; height: 20px;">24.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">3</td>
<td style="text-align: center; width: 35px; height: 20px;">2.2</td>
<td style="text-align: center; width: 135px; height: 20px;">5.25</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">7.5</td>
<td style="text-align: center; width: 39px; height: 20px;">2.25</td>
<td style="text-align: center; width: 42px; height: 20px;">15.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">4</td>
<td style="text-align: center; width: 35px; height: 20px;">1.5</td>
<td style="text-align: center; width: 135px; height: 20px;">2.65</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">5.0</td>
<td style="text-align: center; width: 39px; height: 20px;">2.35</td>
<td style="text-align: center; width: 42px; height: 20px;">10.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">5</td>
<td style="text-align: center; width: 35px; height: 20px;">1.2</td>
<td style="text-align: center; width: 135px; height: 20px;">1.40</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">4.2</td>
<td style="text-align: center; width: 39px; height: 20px;">2.80 </td>
<td style="text-align: center; width: 42px; height: 20px;">8.4</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">6</td>
<td style="text-align: center; width: 35px; height: 20px;">1.4</td>
<td style="text-align: center; width: 135px; height: 20px;">0.00</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">5.0</td>
<td style="text-align: center; width: 39px; height: 20px;">5.00</td>
<td style="text-align: center; width: 42px; height: 20px;">10.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;"> </td>
<td style="text-align: center; width: 35px; height: 20px;"> 11.1</td>
<td style="text-align: center; width: 135px; height: 20px;"> </td>
<td style="text-align: center; width: 58px; height: 20px;"> </td>
<td style="text-align: center; width: 58px; height: 20px;"> </td>
<td style="text-align: center; width: 39px; height: 20px;"> </td>
<td style="text-align: center; width: 42px; height: 20px;"> </td>
</tr>
</tbody>
</table>
<p> </p>
<p>16)    Linear log sucrose gradient for SW41 rotor tubes, 14°C, Pp=1.30 for 6 gradients</p>
<p> </p>
<table>
<tbody>
<tr style="height: 24px;">
<td style="height: 24px; text-align: center;"><strong>Tube</strong></td>
<td style="height: 24px; text-align: center;"><strong>Layer</strong></td>
<td style="height: 24px; text-align: center;"><strong>Sucrose (600 mg/ml)</strong></td>
<td style="height: 24px; text-align: center;"><strong>2X buffer</strong></td>
<td style="height: 24px; text-align: center;"><strong>d-H<sub>2</sub>O</strong></td>
<td style="height: 24px; text-align: center;"><strong>Total</strong></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
<td style="height: 20px; text-align: center;"><em>Volume</em></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">1</td>
<td style="height: 20px; text-align: center;">1.5</td>
<td style="height: 20px; text-align: center;">6.0</td>
<td style="height: 20px; text-align: center;">6.0</td>
<td style="height: 20px; text-align: center;">---</td>
<td style="height: 20px; text-align: center;">12.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">2</td>
<td style="height: 20px; text-align: center;">3.2</td>
<td style="height: 20px; text-align: center;">10.4</td>
<td style="height: 20px; text-align: center;">12.0</td>
<td style="height: 20px; text-align: center;">1.6  </td>
<td style="height: 20px; text-align: center;">24.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">3</td>
<td style="height: 20px; text-align: center;">2.7</td>
<td style="height: 20px; text-align: center;">7.0</td>
<td style="height: 20px; text-align: center;">10.0</td>
<td style="height: 20px; text-align: center;">3.0</td>
<td style="height: 20px; text-align: center;">20.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">4</td>
<td style="height: 20px; text-align: center;">1.8</td>
<td style="height: 20px; text-align: center;">4.0</td>
<td style="height: 20px; text-align: center;">7.5</td>
<td style="height: 20px; text-align: center;">3.5</td>
<td style="height: 20px; text-align: center;">15.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">5</td>
<td style="height: 20px; text-align: center;">1.3</td>
<td style="height: 20px; text-align: center;">1.7</td>
<td style="height: 20px; text-align: center;">5.0</td>
<td style="height: 20px; text-align: center;">3.3</td>
<td style="height: 20px; text-align: center;">10.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">6</td>
<td style="height: 20px; text-align: center;">1.1</td>
<td style="height: 20px; text-align: center;">0.0</td>
<td style="height: 20px; text-align: center;">5.0</td>
<td style="height: 20px; text-align: center;">5.0 </td>
<td style="height: 20px; text-align: center;">10.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;">11.6 </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
</tr>
</tbody>
</table>
<p>     </p>
<p>17)    Linear log sucrose gradient for SW50.1 rotor tubes, 14°C, Pp=1.50 for 6 gradients<br /><br /></p>
<table>
<tbody>
<tr style="height: 24px;">
<td style="text-align: center; width: 31px; height: 24px;"><strong>Tube</strong></td>
<td style="text-align: center; width: 35px; height: 24px;"><strong>Layer</strong></td>
<td style="text-align: center; width: 135px; height: 24px;"><strong>Sucrose (600 mg/ml)</strong></td>
<td style="text-align: center; width: 58px; height: 24px;"><strong>4X buffer</strong></td>
<td style="text-align: center; width: 58px; height: 24px;"><strong>2X buffer</strong></td>
<td style="text-align: center; width: 39px; height: 24px;"><strong>d-H<sub>2</sub>O</strong></td>
<td style="text-align: center; width: 42px; height: 24px;"><strong>Total</strong></td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;"> </td>
<td style="text-align: center; width: 35px; height: 20px;"><em>(mL)</em></td>
<td style="text-align: center; width: 135px; height: 20px;"><em> (mL)</em></td>
<td style="text-align: center; width: 58px; height: 20px;"><em>(mL)</em></td>
<td style="text-align: center; width: 58px; height: 20px;"><em>(mL)</em></td>
<td style="text-align: center; width: 39px; height: 20px;"><em>(mL)</em></td>
<td style="text-align: center; width: 42px; height: 20px;"><em>Volume</em></td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">1</td>
<td style="text-align: center; width: 35px; height: 20px;">0.92</td>
<td style="text-align: center; width: 135px; height: 20px;">5.40</td>
<td style="text-align: center; width: 58px; height: 20px;">2.5</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 39px; height: 20px;">2.10</td>
<td style="text-align: center; width: 42px; height: 20px;">10.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">2</td>
<td style="text-align: center; width: 35px; height: 20px;">1.66</td>
<td style="text-align: center; width: 135px; height: 20px;">6.75</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">7.5</td>
<td style="text-align: center; width: 39px; height: 20px;">0.75</td>
<td style="text-align: center; width: 42px; height: 20px;">15.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">3</td>
<td style="text-align: center; width: 35px; height: 20px;">1.15</td>
<td style="text-align: center; width: 135px; height: 20px;">5.25</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">7.5</td>
<td style="text-align: center; width: 39px; height: 20px;">2.25</td>
<td style="text-align: center; width: 42px; height: 20px;">15.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">4</td>
<td style="text-align: center; width: 35px; height: 20px;">0.65</td>
<td style="text-align: center; width: 135px; height: 20px;">2.15</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">4.0</td>
<td style="text-align: center; width: 39px; height: 20px;">1.85</td>
<td style="text-align: center; width: 42px; height: 20px;">8.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">5</td>
<td style="text-align: center; width: 35px; height: 20px;">0.55</td>
<td style="text-align: center; width: 135px; height: 20px;">1.60</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">4.0</td>
<td style="text-align: center; width: 39px; height: 20px;">2.40 </td>
<td style="text-align: center; width: 42px; height: 20px;">8.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">6</td>
<td style="text-align: center; width: 35px; height: 20px;">0.37</td>
<td style="text-align: center; width: 135px; height: 20px;">0.00</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">3.0</td>
<td style="text-align: center; width: 39px; height: 20px;">3.00</td>
<td style="text-align: center; width: 42px; height: 20px;">6.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;"> </td>
<td style="text-align: center; width: 35px; height: 20px;"> 5.30</td>
<td style="text-align: center; width: 135px; height: 20px;"> </td>
<td style="text-align: center; width: 58px; height: 20px;"> </td>
<td style="text-align: center; width: 58px; height: 20px;"> </td>
<td style="text-align: center; width: 39px; height: 20px;"> </td>
<td style="text-align: center; width: 42px; height: 20px;"> </td>
</tr>
</tbody>
</table>
<p>  </p>
<p>18)    Linear log sucrose gradient for SW60 rotor tubes, 14°C, Pp=1.50 for 6 gradients</p>
<p> </p>
<table>
<tbody>
<tr style="height: 24px;">
<td style="text-align: center; width: 31px; height: 24px;"><strong>Tube</strong></td>
<td style="text-align: center; width: 35px; height: 24px;"><strong>Layer</strong></td>
<td style="text-align: center; width: 135px; height: 24px;"><strong>Sucrose (600 mg/ml)</strong></td>
<td style="text-align: center; width: 58px; height: 24px;"><strong>4X buffer</strong></td>
<td style="text-align: center; width: 58px; height: 24px;"><strong>2X buffer</strong></td>
<td style="text-align: center; width: 39px; height: 24px;"><strong>d-H<sub>2</sub>O</strong></td>
<td style="text-align: center; width: 42px; height: 24px;"><strong>Total</strong></td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;"> </td>
<td style="text-align: center; width: 35px; height: 20px;"><em>(mL)</em></td>
<td style="text-align: center; width: 135px; height: 20px;"><em> (mL)</em></td>
<td style="text-align: center; width: 58px; height: 20px;"><em>(mL)</em></td>
<td style="text-align: center; width: 58px; height: 20px;"><em>(mL)</em></td>
<td style="text-align: center; width: 39px; height: 20px;"><em>(mL)</em></td>
<td style="text-align: center; width: 42px; height: 20px;"><em>Volume</em></td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">1</td>
<td style="text-align: center; width: 35px; height: 20px;">0.68</td>
<td style="text-align: center; width: 135px; height: 20px;">6.5</td>
<td style="text-align: center; width: 58px; height: 20px;">3.0</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 39px; height: 20px;">2.5</td>
<td style="text-align: center; width: 42px; height: 20px;">12.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">2</td>
<td style="text-align: center; width: 35px; height: 20px;">1.41</td>
<td style="text-align: center; width: 135px; height: 20px;">5.4</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">6.0</td>
<td style="text-align: center; width: 39px; height: 20px;">0.6</td>
<td style="text-align: center; width: 42px; height: 20px;">12.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">3</td>
<td style="text-align: center; width: 35px; height: 20px;">0.91</td>
<td style="text-align: center; width: 135px; height: 20px;">4.2</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">6.0</td>
<td style="text-align: center; width: 39px; height: 20px;">1.8</td>
<td style="text-align: center; width: 42px; height: 20px;">12.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">4</td>
<td style="text-align: center; width: 35px; height: 20px;">0.67</td>
<td style="text-align: center; width: 135px; height: 20px;">3.2</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">6.0</td>
<td style="text-align: center; width: 39px; height: 20px;">2.8</td>
<td style="text-align: center; width: 42px; height: 20px;">12.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">5</td>
<td style="text-align: center; width: 35px; height: 20px;">0.44</td>
<td style="text-align: center; width: 135px; height: 20px;">1.6</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">6.0</td>
<td style="text-align: center; width: 39px; height: 20px;">4.4 </td>
<td style="text-align: center; width: 42px; height: 20px;">12.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;">6</td>
<td style="text-align: center; width: 35px; height: 20px;">0.29</td>
<td style="text-align: center; width: 135px; height: 20px;">0.0</td>
<td style="text-align: center; width: 58px; height: 20px;">---</td>
<td style="text-align: center; width: 58px; height: 20px;">6.0</td>
<td style="text-align: center; width: 39px; height: 20px;">6.0</td>
<td style="text-align: center; width: 42px; height: 20px;">12.0</td>
</tr>
<tr style="height: 20px;">
<td style="text-align: center; width: 31px; height: 20px;"> </td>
<td style="text-align: center; width: 35px; height: 20px;"> 4.40</td>
<td style="text-align: center; width: 135px; height: 20px;"> </td>
<td style="text-align: center; width: 58px; height: 20px;"> </td>
<td style="text-align: center; width: 58px; height: 20px;"> </td>
<td style="text-align: center; width: 39px; height: 20px;"> </td>
<td style="text-align: center; width: 42px; height: 20px;"> </td>
</tr>
</tbody>
</table>
<p>         </p>
<p>19)    Linear log sucrose gradient for SW50.1 rotor tubes, 14°C, Pp=1.50 for 6 gradients</p>
<p> </p>
<table>
<tbody>
<tr style="height: 24px;">
<td style="height: 24px; text-align: center;"><strong>Tube</strong></td>
<td style="height: 24px; text-align: center;"><strong>Layer</strong></td>
<td style="height: 24px; text-align: center;"><strong>Sucrose (600 mg/ml)</strong></td>
<td style="height: 24px; text-align: center;"><strong>2X buffer</strong></td>
<td style="height: 24px; text-align: center;"><strong>1X buffer</strong></td>
<td style="height: 24px; text-align: center;"><strong>Total</strong></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
<td style="height: 20px; text-align: center;"><em>Volume</em></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">1</td>
<td style="height: 20px; text-align: center;">1.10</td>
<td style="height: 20px; text-align: center;">4.93</td>
<td style="height: 20px; text-align: center;">4.93</td>
<td style="height: 20px; text-align: center;">0.14</td>
<td style="height: 20px; text-align: center;">10.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">2</td>
<td style="height: 20px; text-align: center;">1.80</td>
<td style="height: 20px; text-align: center;">5.50</td>
<td style="height: 20px; text-align: center;">5.50</td>
<td style="height: 20px; text-align: center;">4.00</td>
<td style="height: 20px; text-align: center;">15.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">3</td>
<td style="height: 20px; text-align: center;">1.08</td>
<td style="height: 20px; text-align: center;">2.83</td>
<td style="height: 20px; text-align: center;">2.83</td>
<td style="height: 20px; text-align: center;">4.34</td>
<td style="height: 20px; text-align: center;">10.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">4</td>
<td style="height: 20px; text-align: center;">0.72</td>
<td style="height: 20px; text-align: center;">1.50</td>
<td style="height: 20px; text-align: center;">1.50</td>
<td style="height: 20px; text-align: center;">7.00</td>
<td style="height: 20px; text-align: center;">10.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">5</td>
<td style="height: 20px; text-align: center;">0.60</td>
<td style="height: 20px; text-align: center;">0.00</td>
<td style="height: 20px; text-align: center;">0.00</td>
<td style="height: 20px; text-align: center;">5.00</td>
<td style="height: 20px; text-align: center;">5.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;">5.30 </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
</tr>
</tbody>
</table>
<p> </p>
<p>20)    20-40% (w/w) linear CsCl gradient for SW60 rotor tubes for 6 gradients for virus</p>
<p> </p>
<table>
<tbody>
<tr style="height: 24px;">
<td style="height: 24px; text-align: center;"><strong>Tube</strong></td>
<td style="height: 24px; text-align: center;"><strong>Percent</strong></td>
<td style="height: 24px; text-align: center;"><strong>CsCl</strong></td>
<td style="height: 24px; text-align: center;"><strong>1X buffer</strong></td>
<td style="height: 24px; text-align: center;"><strong>Layer</strong></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"><em>(gm)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">1</td>
<td style="height: 20px; text-align: center;">40.0</td>
<td style="height: 20px; text-align: center;">5.0</td>
<td style="height: 20px; text-align: center;">7.5</td>
<td style="height: 20px; text-align: center;">1.1</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">2</td>
<td style="height: 20px; text-align: center;">33.5</td>
<td style="height: 20px; text-align: center;">4.0</td>
<td style="height: 20px; text-align: center;">7.9</td>
<td style="height: 20px; text-align: center;">1.1</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">3</td>
<td style="height: 20px; text-align: center;">26.5</td>
<td style="height: 20px; text-align: center;">3.0</td>
<td style="height: 20px; text-align: center;">8.3</td>
<td style="height: 20px; text-align: center;">1.1</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">4</td>
<td style="height: 20px; text-align: center;">20.0</td>
<td style="height: 20px; text-align: center;">2.0</td>
<td style="height: 20px; text-align: center;">8.0</td>
<td style="height: 20px; text-align: center;">1.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;">4.3</td>
</tr>
</tbody>
</table>
<p>                  Centrifuge at 40,000 rpm, 4 hours, 4°C<br /><br /></p>
<p>21)    20-50% (w/w) linear CsCl gradients for SW60 rotor tubes for 6 gradients for virus</p>
<p> </p>
<table>
<tbody>
<tr style="height: 24px;">
<td style="height: 24px; text-align: center;"><strong>Tube</strong></td>
<td style="height: 24px; text-align: center;"><strong>Percent</strong></td>
<td style="height: 24px; text-align: center;"><strong>CsCl</strong></td>
<td style="height: 24px; text-align: center;"><strong>1X buffer</strong></td>
<td style="height: 24px; text-align: center;"><strong>Layer</strong></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"><em>(gm)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">1</td>
<td style="height: 20px; text-align: center;">50.0</td>
<td style="height: 20px; text-align: center;">7.5</td>
<td style="height: 20px; text-align: center;">7.5</td>
<td style="height: 20px; text-align: center;">1.1</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">2</td>
<td style="height: 20px; text-align: center;">40.0</td>
<td style="height: 20px; text-align: center;">5.0</td>
<td style="height: 20px; text-align: center;">7.5</td>
<td style="height: 20px; text-align: center;">1.1</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">3</td>
<td style="height: 20px; text-align: center;">30.0</td>
<td style="height: 20px; text-align: center;">3.0</td>
<td style="height: 20px; text-align: center;">7.0</td>
<td style="height: 20px; text-align: center;">1.1</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">4</td>
<td style="height: 20px; text-align: center;">20.0</td>
<td style="height: 20px; text-align: center;">2.0</td>
<td style="height: 20px; text-align: center;">8.0</td>
<td style="height: 20px; text-align: center;">1.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;">4.3</td>
</tr>
</tbody>
</table>
<p>                  Centrifuge at 40,000 rpm, 18 hours, 25°C</p>
<p> </p>
<p>22)    30-60% (w/w) linear CsCl gradient for SW60 rotor tubes for 6 gradients for DNA</p>
<p> </p>
<table>
<tbody>
<tr style="height: 24px;">
<td style="height: 24px; text-align: center;"><strong>Tube</strong></td>
<td style="height: 24px; text-align: center;"><strong>Percent</strong></td>
<td style="height: 24px; text-align: center;"><strong>CsCl</strong></td>
<td style="height: 24px; text-align: center;"><strong>1X buffer</strong></td>
<td style="height: 24px; text-align: center;"><strong>Hoechst dye</strong></td>
<td style="height: 24px; text-align: center;"><strong>Layer</strong></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"><em>(gm)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
<td style="height: 20px; text-align: center;"><em>#33258 (µL)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">1</td>
<td style="height: 20px; text-align: center;">60.0</td>
<td style="height: 20px; text-align: center;">9.0</td>
<td style="height: 20px; text-align: center;">6.0</td>
<td style="height: 20px; text-align: center;">60.0</td>
<td style="height: 20px; text-align: center;">1.1</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">2</td>
<td style="height: 20px; text-align: center;">50.0</td>
<td style="height: 20px; text-align: center;">7.5</td>
<td style="height: 20px; text-align: center;">7.5</td>
<td style="height: 20px; text-align: center;">75.0</td>
<td style="height: 20px; text-align: center;">1.05</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">3</td>
<td style="height: 20px; text-align: center;">40.0</td>
<td style="height: 20px; text-align: center;">5.0</td>
<td style="height: 20px; text-align: center;">7.5</td>
<td style="height: 20px; text-align: center;">75.0</td>
<td style="height: 20px; text-align: center;">1.05</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">4</td>
<td style="height: 20px; text-align: center;">30.0</td>
<td style="height: 20px; text-align: center;">3.0</td>
<td style="height: 20px; text-align: center;">7.0</td>
<td style="height: 20px; text-align: center;">70.0</td>
<td style="height: 20px; text-align: center;">1.2</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;">4.4</td>
</tr>
</tbody>
</table>
<p>                  Centrifuge at 35,000 rpm, 18 hours, 25°C</p>
<p> </p>
<p>23)    45-60% (w/w) linear CsCl gradient for SW60 rotor tubes for 6 gradients for DNA</p>
<p> </p>
<table>
<tbody>
<tr style="height: 24px;">
<td style="height: 24px; text-align: center;"><strong>Tube</strong></td>
<td style="height: 24px; text-align: center;"><strong>Percent</strong></td>
<td style="height: 24px; text-align: center;"><strong>CsCl</strong></td>
<td style="height: 24px; text-align: center;"><strong>1X buffer</strong></td>
<td style="height: 24px; text-align: center;"><strong>Hoechst dye</strong></td>
<td style="height: 24px; text-align: center;"><strong>Layer</strong></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"><em>(gm)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
<td style="height: 20px; text-align: center;"><em>#33258 (µL)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">1</td>
<td style="height: 20px; text-align: center;">60.0</td>
<td style="height: 20px; text-align: center;">9.0</td>
<td style="height: 20px; text-align: center;">6.0</td>
<td style="height: 20px; text-align: center;">60.0</td>
<td style="height: 20px; text-align: center;">1.1</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">2</td>
<td style="height: 20px; text-align: center;">55.0</td>
<td style="height: 20px; text-align: center;">9.0</td>
<td style="height: 20px; text-align: center;">7.4</td>
<td style="height: 20px; text-align: center;">74.0</td>
<td style="height: 20px; text-align: center;">1.05</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">3</td>
<td style="height: 20px; text-align: center;">50.0</td>
<td style="height: 20px; text-align: center;">7.5</td>
<td style="height: 20px; text-align: center;">7.5</td>
<td style="height: 20px; text-align: center;">75.0</td>
<td style="height: 20px; text-align: center;">1.05</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">4</td>
<td style="height: 20px; text-align: center;">45.0</td>
<td style="height: 20px; text-align: center;">6.0</td>
<td style="height: 20px; text-align: center;">7.3</td>
<td style="height: 20px; text-align: center;">73.0</td>
<td style="height: 20px; text-align: center;">1.2</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;">4.4</td>
</tr>
</tbody>
</table>
<p>                  Centrifuge at 30,000 rpm, 24 hours, 25°C<br /><br /></p>
<p>24)    17-35% (w/w) linear Cs<sub>2</sub>SO<sub>4</sub> gradients for SW60 rotor tubes for 6 gradients for virus</p>
<p> </p>
<table>
<tbody>
<tr style="height: 24px;">
<td style="height: 24px; text-align: center;"><strong>Tube</strong></td>
<td style="height: 24px; text-align: center;"><strong>Percent</strong></td>
<td style="height: 24px; text-align: center;"><strong>Cs<sub>2</sub>SO<sub>4</sub></strong></td>
<td style="height: 24px; text-align: center;"><strong>1X buffer</strong></td>
<td style="height: 24px; text-align: center;"><strong>Layer</strong></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"><em>(gm)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
<td style="height: 20px; text-align: center;"><em>(mL)</em></td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">1</td>
<td style="height: 20px; text-align: center;">35.0</td>
<td style="height: 20px; text-align: center;">4.2</td>
<td style="height: 20px; text-align: center;">7.8</td>
<td style="height: 20px; text-align: center;">1.1</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">2</td>
<td style="height: 20px; text-align: center;">29.0</td>
<td style="height: 20px; text-align: center;">3.2</td>
<td style="height: 20px; text-align: center;">7.8</td>
<td style="height: 20px; text-align: center;">1.1</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">3</td>
<td style="height: 20px; text-align: center;">23.0</td>
<td style="height: 20px; text-align: center;">2.3</td>
<td style="height: 20px; text-align: center;">7.7</td>
<td style="height: 20px; text-align: center;">1.1</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;">4</td>
<td style="height: 20px; text-align: center;">17.0</td>
<td style="height: 20px; text-align: center;">1.7</td>
<td style="height: 20px; text-align: center;">8.3</td>
<td style="height: 20px; text-align: center;">1.0</td>
</tr>
<tr style="height: 20px;">
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;"> </td>
<td style="height: 20px; text-align: center;">4.3</td>
</tr>
</tbody>
</table>
<p>                  Centrifuge at 40,000 rpm, 18 hours, 25°C</p>
<p><br /> </p>
<p><br /> </p>

[STEPS]