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<p>This is a collection of protocols from 'Flower Ca<sup>2+</sup> channel in CME and ADBE' of Yao CK et al. (2017).</p>
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<p>Please see the manuscript for the full method details.</p>
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<p><strong>References:</strong></p>
<ol>
<li>Haucke V, Neher E, Sigrist SJ (2011) Protein scaffolds in the coupling of synaptic exocytosis and endocytosis. Nat Rev Neurosci 12: 127-138.</li>
<li>Wu LG, Hamid E, Shin W, Chiang HC (2014) Exocytosis and endocytosis: modes, functions, and coupling mechanisms. Annu Rev Physiol 76: 301-331.</li>
<li>Kononenko NL, Haucke V (2015) Molecular mechanisms of presynaptic membrane retrieval and synaptic vesicle reformation. Neuron 85: 484-496.</li>
<li>Saheki Y, De Camilli P (2012) Synaptic vesicle endocytosis. Cold Spring Harb Perspect Biol 4: a005645.</li>
<li>Granseth B, Odermatt B, Royle SJ, Lagnado L (2006) Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses. Neuron 51: 773-786.</li>
<li>Zhu Y, Xu J, Heinemann SF (2009) Two pathways of synaptic vesicle retrieval revealed by single-vesicle imaging. Neuron 61: 397-411.</li>
<li>Wu W, Wu LG (2007) Rapid bulk endocytosis and its kinetics of fission pore closure at a central synapse. Proc Natl Acad Sci U S A 104: 10234-10239.</li>
<li>Clayton EL, Evans GJ, Cousin MA (2008) Bulk synaptic vesicle endocytosis is rapidly triggered during strong stimulation. J Neurosci 28: 6627-6632.</li>
<li>Littleton JT, Stern M, Schulze K, Perin M, Bellen HJ (1993) Mutational analysis of Drosophila synaptotagmin demonstrates its essential role in Ca(2+)-activated neurotransmitter release. Cell 74: 1125-1134.</li>
<li>Poskanzer KE, Marek KW, Sweeney ST, Davis GW (2003) Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo. Nature 426: 559-563.</li>
<li>Xu J, Luo F, Zhang Z, Xue L, Wu XS, et al. (2013) SNARE proteins synaptobrevin, SNAP-25, and syntaxin are involved in rapid and slow endocytosis at synapses. Cell Rep 3: 1414-1421.</li>
<li>Yao J, Kwon SE, Gaffaney JD, Dunning FM, Chapman ER (2012) Uncoupling the roles of synaptotagmin I during endo- and exocytosis of synaptic vesicles. Nat Neurosci 15: 243-249.</li>
<li>Poskanzer KE, Fetter RD, Davis GW (2006) Discrete residues in the c(2)b domain of synaptotagmin I independently specify endocytic rate and synaptic vesicle size. Neuron 50: 49-62.</li>
<li>Nicholson-Tomishima K, Ryan TA (2004) Kinetic efficiency of endocytosis at mammalian CNS synapses requires synaptotagmin I. Proc Natl Acad Sci U S A 101: 16648-16652.</li>
<li>Nicholson-Fish JC, Kokotos AC, Gillingwater TH, Smillie KJ, Cousin MA (2015) VAMP4 Is an Essential Cargo Molecule for Activity-Dependent Bulk Endocytosis. Neuron 88: 973-984.</li>
<li>Gad H, Low P, Zotova E, Brodin L, Shupliakov O (1998) Dissociation between Ca2+-triggered synaptic vesicle exocytosis and clathrin-mediated endocytosis at a central synapse. Neuron 21: 607-616.</li>
<li>Hosoi N, Holt M, Sakaba T (2009) Calcium dependence of exo- and endocytotic coupling at a glutamatergic synapse. Neuron 63: 216-229.</li>
<li>Wu XS, McNeil BD, Xu J, Fan J, Xue L, et al. (2009) Ca(2+) and calmodulin initiate all forms of endocytosis during depolarization at a nerve terminal. Nat Neurosci 12: 1003-1010.</li>
<li>Marks B, McMahon HT (1998) Calcium triggers calcineurin-dependent synaptic vesicle recycling in mammalian nerve terminals. Curr Biol 8: 740-749.</li>
<li>Sankaranarayanan S, Ryan TA (2001) Calcium accelerates endocytosis of vSNAREs at hippocampal synapses. Nat Neurosci 4: 129-136.</li>
<li>Wu XS, Zhang Z, Zhao WD, Wang D, Luo F, et al. (2014) Calcineurin is universally involved in vesicle endocytosis at neuronal and nonneuronal secretory cells. Cell Rep 7: 982-988.</li>
<li>Sun T, Wu XS, Xu J, McNeil BD, Pang ZP, et al. (2010) The role of calcium/calmodulin-activated calcineurin in rapid and slow endocytosis at central synapses. J Neurosci 30: 11838-11847.</li>
<li>Xue L, Zhang Z, McNeil BD, Luo F, Wu XS, et al. (2012) Voltage-dependent calcium channels at the plasma membrane, but not vesicular channels, couple exocytosis to endocytosis. Cell Rep 1: 632-638.</li>
<li>Yao CK, Lin YQ, Ly CV, Ohyama T, Haueter CM, et al. (2009) A synaptic vesicle-associated Ca2+ channel promotes endocytosis and couples exocytosis to endocytosis. Cell 138: 947-960.</li>
<li>Jan LY, Jan YN (1982) Antibodies to horseradish peroxidase as specific neuronal markers in Drosophila and in grasshopper embryos. Proc Natl Acad Sci U S A 79: 2700-2704.</li>
<li>Morton A, Marland JR, Cousin MA (2015) Synaptic vesicle exocytosis and increased cytosolic calcium are both necessary but not sufficient for activity-dependent bulk endocytosis. J Neurochem 134: 405-415.</li>
<li>Akbergenova Y, Bykhovskaia M (2009) Enhancement of the endosomal endocytic pathway increases quantal size. Mol Cell Neurosci 40: 199-206.</li>
<li>Stevens RJ, Akbergenova Y, Jorquera RA, Littleton JT (2012) Abnormal synaptic vesicle biogenesis in Drosophila synaptogyrin mutants. J Neurosci 32: 18054-18067, 18067a.</li>
<li>Vijayakrishnan N, Woodruff EA, 3rd, Broadie K (2009) Rolling blackout is required for bulk endocytosis in non-neuronal cells and neuronal synapses. J Cell Sci 122: 114-125.</li>
<li>Meunier FA, Nguyen TH, Colasante C, Luo F, Sullivan RK, et al. (2010) Sustained synaptic-vesicle recycling by bulk endocytosis contributes to the maintenance of high-rate neurotransmitter release stimulated by glycerotoxin. J Cell Sci 123: 1131-1140.</li>
<li>Kasprowicz J, Kuenen S, Miskiewicz K, Habets RL, Smitz L, et al. (2008) Inactivation of clathrin heavy chain inhibits synaptic recycling but allows bulk membrane uptake. J Cell Biol 182: 1007-1016.</li>
<li>Heerssen H, Fetter RD, Davis GW (2008) Clathrin dependence of synaptic-vesicle formation at the Drosophila neuromuscular junction. Curr Biol 18: 401-409.</li>
<li>Kasprowicz J, Kuenen S, Swerts J, Miskiewicz K, Verstreken P (2014) Dynamin photoinactivation blocks Clathrin and alpha-adaptin recruitment and induces bulk membrane retrieval. J Cell Biol 204: 1141-1156.</li>
<li>Vanlandingham PA, Barmchi MP, Royer S, Green R, Bao H, et al. (2014) AP180 couples protein retrieval to clathrin-mediated endocytosis of synaptic vesicles. Traffic 15: 433-450.</li>
<li>Chen TW, Wardill TJ, Sun Y, Pulver SR, Renninger SL, et al. (2013) Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499: 295-300.</li>
<li>Koh TW, Verstreken P, Bellen HJ (2004) Dap160/intersectin acts as a stabilizing scaffold required for synaptic development and vesicle endocytosis. Neuron 43: 193-205.</li>
<li>Koh TW, Korolchuk VI, Wairkar YP, Jiao W, Evergren E, et al. (2007) Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development. J Cell Biol 178: 309-322.</li>
<li>Marie B, Sweeney ST, Poskanzer KE, Roos J, Kelly RB, et al. (2004) Dap160/intersectin scaffolds the periactive zone to achieve high-fidelity endocytosis and normal synaptic growth. Neuron 43: 207-219.</li>
<li>Atlas D (2013) The voltage-gated calcium channel functions as the molecular switch of synaptic transmission. Annu Rev Biochem 82: 607-635.</li>
<li>Sather WA, McCleskey EW (2003) Permeation and selectivity in calcium channels. Annu Rev Physiol 65: 133-159.</li>
<li>Petrova E, Lopez-Gay JM, Rhiner C, Moreno E (2012) Flower-deficient mice have reduced susceptibility to skin papilloma formation. Dis Model Mech 5: 553-561.</li>
<li>Chao HW, Hong CJ, Huang TN, Lin YL, Hsueh YP (2008) SUMOylation of the MAGUK protein CASK regulates dendritic spinogenesis. J Cell Biol 182: 141-155.</li>
<li>Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, et al. (2013) Genome engineering using the CRISPR-Cas9 system. Nat Protoc 8: 2281-2308.</li>
<li>Willox AK, Royle SJ (2012) Stonin 2 is a major adaptor protein for clathrin-mediated synaptic vesicle retrieval. Curr Biol 22: 1435-1439.</li>
<li>Renden R, von Gersdorff H (2007) Synaptic vesicle endocytosis at a CNS nerve terminal: faster kinetics at physiological temperatures and increased endocytotic capacity during maturation. J Neurophysiol 98: 3349-3359.</li>
<li>Nouvian R (2007) Temperature enhances exocytosis efficiency at the mouse inner hair cell ribbon synapse. J Physiol 584: 535-542.</li>
<li>Atluri PP, Ryan TA (2006) The kinetics of synaptic vesicle reacidification at hippocampal nerve terminals. J Neurosci 26: 2313-2320.</li>
<li>Kwon SE, Chapman ER (2011) Synaptophysin regulates the kinetics of synaptic vesicle endocytosis in central neurons. Neuron 70: 847-854.</li>
<li>Wenzel EM, Morton A, Ebert K, Welzel O, Kornhuber J, et al. (2012) Key physiological parameters dictate triggering of activity-dependent bulk endocytosis in hippocampal synapses. PLoS One 7: e38188.</li>
<li>Winther AM, Jiao W, Vorontsova O, Rees KA, Koh TW, et al. (2013) The dynamin-binding domains of Dap160/intersectin affect bulk membrane retrieval in synapses. J Cell Sci 126: 1021-1031.</li>
<li>Rodal AA, Blunk AD, Akbergenova Y, Jorquera RA, Buhl LK, et al. (2011) A presynaptic endosomal trafficking pathway controls synaptic growth signaling. J Cell Biol 193: 201-217.</li>
<li>Kawasaki F, Hazen M, Ordway RW (2000) Fast synaptic fatigue in shibire mutants reveals a rapid requirement for dynamin in synaptic vesicle membrane trafficking. Nat Neurosci 3: 859-860.</li>
<li>Takamori S, Holt M, Stenius K, Lemke EA, Gronborg M, et al. (2006) Molecular anatomy of a trafficking organelle. Cell 127: 831-846.</li>
<li>Vig M, Beck A, Billingsley JM, Lis A, Parvez S, et al. (2006) CRACM1 multimers form the ion-selective pore of the CRAC channel. Curr Biol 16: 2073-2079.</li>
<li>Owsianik G, Talavera K, Voets T, Nilius B (2006) Permeation and selectivity of TRP channels. Annu Rev Physiol 68: 685-717.</li>
<li>Ohyama T, Verstreken P, Ly CV, Rosenmund T, Rajan A, et al. (2007) Huntingtin-interacting protein 14, a palmitoyl transferase required for exocytosis and targeting of CSP to synaptic vesicles. J Cell Biol 179: 1481-1496.</li>
<li>Parnas D, Haghighi AP, Fetter RD, Kim SW, Goodman CS (2001) Regulation of postsynaptic structure and protein localization by the Rho-type guanine nucleotide exchange factor dPix. Neuron 32: 415-424.</li>
<li>Lahey T, Gorczyca M, Jia XX, Budnik V (1994) The Drosophila tumor suppressor gene dlg is required for normal synaptic bouton structure. Neuron 13: 823-835.</li>
<li>Wagh DA, Rasse TM, Asan E, Hofbauer A, Schwenkert I, et al. (2006) Bruchpilot, a protein with homology to ELKS/CAST, is required for structural integrity and function of synaptic active zones in Drosophila. Neuron 49: 833-844.</li>
<li>Stewart BA, Atwood HL, Renger JJ, Wang J, Wu CF (1994) Improved stability of Drosophila larval neuromuscular preparations in haemolymph-like physiological solutions. J Comp Physiol A 175: 179-191.</li>
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