TITLE: Viral inactivation of clinical samples
AUTHORS: Tammy Krylova, Tim Budd, Jerome Nicod, Rupert Beale, Simon Caidan

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Purpose of examination / Clinical relevance</span></div><div class = "text-block"><div class = "justify" style = "text-align:left">At the end of 2019, several pneumonia cases were reported in Wuhan, China and the pathogen was confirmed as a new viral strain. World Health organization has named the newly identified coronavirus as 2019-nCoV, also known as SARS-Cov-2. The disease developed into a dangerous pandemic, posing major challenges to the NHS. Although more research is necessary to better understand the virus, in response to the emergency, simple and rapid testing is essential to identify the virus in infected individuals. This will aid the implementation of efficient interventions to contain the spread, and distinguish healthcare workers who have been infected, and are required to self-isolate, from those showing similar symptoms but which are not 2019-nCoV associated. The latter category may continue to work, alleviating stress on hard-pressed healthcare resources. 2019-nCoV is an RNA virus, and the diagnostic tests detect viral RNA in swabs from patient airways using a reverse transcriptase PCR assay. Samples are submitted to HSL, an accredited reporting laboratory, and transferred to the Crick for testing. The first step of the process is sample receipt at the Crick. This SOP describes the inactivation of the virus.</div></div></div>

[STEPS]
?. [Inactivation step]
Warning! Exposure to SARS-CoV-2 can result in COVID-19.➢ All unsealed work must be undertaken in a class I or Class II Microbiological Safety Cabinet (MBSC).
Single sample per cycle only!Working with multiple samples might lead to errors in sample identification!➢ Work with only one bagged sample at a time
?. [Inactivation step]
Remove the liquid waste container lid and place it to the side in the MBSC.
?. [Inactivation step]
Examine the sample within the bag to ensure no leakages in the bag, on the side of the tube etc.
?. [Inactivation step]
Check the barcodes stapled to the outer bag match the bar code on the swab vial within it.
If barcodes do not align, DO NOT PROCESS. Spray bag out at end of work to be removed and rescanned at scanning station.
?. [Inactivation step]
Remove barcodes and place on the work area.
?. [Inactivation step]
Take a tissue and spray with Amphospray so it is thoroughly soaked.
?. [Inactivation step]
Spray the outer bag.
?. [Inactivation step]
Open the outer bag and spray inside with Amphospray so inner bag is wet.
?. [Inactivation step]
Remove inner bag and discard outer bag into the dry waste bag.
?. [Inactivation step]
Open the inner bag.
?. [Inactivation step]
Spray inside with Amphospray.
?. [Inactivation step]
Open inner bag and remove sample vial by either:a. Tipping sample onto wet tissueb. Pinching the bottom of the sample tube through bag and scrunching up the bag to reveal the sample.
If two samples are present in one bag, take 50% of the liquid from each sample.
?. [Inactivation step]
Wipe sample tube thoroughly with wet tissue and place into the rack.
?. [Inactivation step]
Discard inner bag into dry waste bag.
?. [Inactivation step]
Pick up a new inactivation tube.
?. [Inactivation step]
Check it contains inactivation liquid.Discard any inactivation vial that does not contain inactivation liquid into the liquid waste container.
?. [Inactivation step]
Check provided barcodes for damage & use best-quality barcode (the others are spares).
Downstream automation will only work if the labels are orientated vertically and as straight as possible!
?. [Inactivation step]
Stick barcode label to the inactivation tube as shown.
?. [Inactivation step]
Place the labelled inactivation tube into the rack.
?. [Inactivation step]
Remove swab sample from the rack.
?. [Inactivation step]
Open swab sample lid.
?. [Inactivation step]
Place lid into liquid waste container.
If swab is still affixed to the lid, discard into liquid waste container.
?. [Inactivation step]
Holding the swab vial, place hands over the liquid waste container so the swab vial opening is slightly hanging above the liquid waste container to catch any drips.
Take care to not place the whole swab vial directly over the waste container in case it falls/slips/dropped accidentally.
?. [Inactivation step]
Draw up ~ into a fresh pastette.
[swab vial contents]
Refer to example pastettes with marked 100 μl level.
?. [Inactivation step]
Keep pastette hovering above liquid waste container.
?. [Inactivation step]
Return sample vial to the rack.
?. [Inactivation step]
Pick up inactivation vial.
?. [Inactivation step]
Remove lid and either place it down or pinch between thumb and forefinger.
?. [Inactivation step]
Dispense the pastette’s content into the bottom of the inactivation vial (to minimise bubbling).
?. [Inactivation step]
Seal inactivation vial.
?. [Inactivation step]
Draw up some  into the pastette.
[Surfanios]
?. [Inactivation step]
Discard the pastette into the liquid waste container.
?. [Inactivation step]
Wipe over inactivation vial with Amphospray-soaked paper towel.
?. [Inactivation step]
Flick inactivation vial.
?. [Inactivation step]
Place into Eppendorf rack.
?. [Inactivation step]
Place open swab vial into liquid waste container.
?. [Inactivation step]
Repeat steps 3 - 36 with the next sample.
?. [Inactivation step]
Pick up one sample bag.
?. [Inactivation step]
Pick up one sample bag.
Important information regarding dry swabs!Dry swabs will arrive in a variety of containers e.g.Universal tubes with conical bottomsUniversal tubes with flat bottomsUrine sample pot with flat bottomsIt is important that the SOP instructions are followed to preventCross contaminationMaximise sample recoveryIt is important that the SOP instructions are followed to preventCross contaminationMaximise sample recovery
?. [Inactivation step]
Examine the sample within the bag to ensure no leakages in the bag, on the side of the tube etc.
?. [Inactivation step]
Check the barcodes stapled to the outer bag match the bar code on the swab vial within it.
If barcodes do not align, DO NOT PROCESS. Spray bag out at end of work to be removed and rescanned at scanning station
?. [Inactivation step]
Remove barcodes and place on the work area.
?. [Inactivation step]
Check provided barcodes for damage and use best-quality barcode (the others are spares).
The ASF robot will only work if the labels are orientated vertically and as straight as possible!
?. [Inactivation step]
Check the 2 ml inactivation tube contains .
[L6 lysis buffer]
?. [Inactivation step]
Stick the barcode label to the inactivation tube.
?. [Inactivation step]
Place the labelled inactivation tube into the rack.
?. [Inactivation step]
Take a tissue and spray with Amphospray so it is thoroughly soaked.
?. [Inactivation step]
Spray the outer bag.
?. [Inactivation step]
Open the outer bag.
?. [Inactivation step]
Spray inside the bag with Amphospray so inner bag is wet.
?. [Inactivation step]
Remove inner bag and discard outer bag into the dry waste bag.
?. [Inactivation step]
Open the inner bag.
?. [Inactivation step]
Spray the inner bag with Amphospray.
?. [Inactivation step]
Remove sample vial by either:a. Tipping sample onto wet tissueb. Pinching the bottom of the sample tube through bag and scrunching up the bag to reveal the sample.
?. [Inactivation step]
Wipe sample tube thoroughly with wet tissue and place into the rack.
?. [Inactivation step]
Discard inner bag into dry waste bag.
?. [Inactivation step]
Unscrew the swab container lid.
?. [Inactivation step]
Place the lid in front of the container.
?. [Inactivation step]
Unscrew barcoded 2 ml inactivation tube containing the .
[L6 lysis buffer]
?. [Inactivation step]
Draw up the lysis buffer into a fresh pastette
?. [Inactivation step]
Aspirate the lysis buffer into opened swab vial.
?. [Inactivation step]
Draw up some  into the pastette.
[Surfanios]
?. [Inactivation step]
Discard the pastette into the liquid waste container.
?. [Inactivation step]
Replace the lid on the swab container.
?. [Inactivation step]
Agitate the swab container to ensure the swabs are in contact with the lysis buffer.
?. [Inactivation step]
Replace the lid on the inactivation tube.
?. [Inactivation step]
Proceed in accordance with the bottom shape of the swab pot: If the swab pot is a flat-bottomed container follow case 'flat bottom'. If the swab pot has a conical bottom follow case 'conical bottom'.
?. [Inactivation step]
Place flat-bottomed containers on the angled rack as shown in the picture.
Maximising sample recovery from flat bottomed containers.
?. [Inactivation step]
Make sure the pot is rotated so that the swab is sitting in the lysis buffer.
?. [Inactivation step]
Leaving tubes two spaces apart on rack, proceed to the next sample.
?. [Inactivation step]
Follow steps 34 - 37 only after lysis buffer has been added to last of the sample set you have takeninto the MBSC (usually this will be a set of 12 swab samples).
All swabs must have a minimum of 10 minutes contact with the lysis buffer. Set 10-minute timer!
?. [Inactivation step]
Follow steps 33 - 36 only after lysis buffer has been added to last of the sample set you have takeninto the MBSC (usually this will be a set of 12 swab samples).
All swabs must have a minimum of 10 minutes contact with the lysis buffer. Set 10-minute timer!
?. [Inactivation step]
Remove over-sleeves and outer gloves.
?. [Inactivation step]
Set the timer outside the MBSC for 10 minutes.
?. [Inactivation step]
Outside the MBSC put on a fresh pair of outer gloves.
?. [Inactivation step]
Outside the MBSC put on a fresh pair of over-sleeves.
?. [Inactivation step]
Unscrew barcoded 2 ml inactivation tube.
Working with multiple samples might lead to errors in sample identification➢ Work with only one swab sample at a time➢ Continue with the protocol ONLY after the 10-minute timer has sounded
?. [Inactivation step]
Place 2 ml inactivation tube lid down to the side.
?. [Inactivation step]
Pick up the corresponding swab container.
?. [Inactivation step]
Check the barcodes on the swab container and opened inactivation tube match.
Correct barcode check➢ This is a vital check to ensure the right sample is processed back into its correspondinginactivation tube to avoid misdiagnosis of patient samples
?. [Inactivation step]
Once the match is verified, unscrew the swab container lid.
?. [Inactivation step]
Discard the swab container lid into the 10% Surfanios liquid waste tub.
?. [Inactivation step]
Holding the swab vial, place hands over the liquid waste container so the swab vial opening is slightly hanging above the liquid waste container to catch any drips.
Take care to not place the whole swab vial directly over the waste container in case it falls/slips/dropped accidentally.
?. [Inactivation step]
Draw up the lysis buffer from the swab container into a fresh pastette.
Important information: the dry swabs will have absorbed some of the lysis buffer➢ Do not expect to recover the full 1 ml of lysis buffer
?. [Inactivation step]
Dispose of the swab container into the 10% Surfanios liquid waste tub.
?. [Inactivation step]
Dispense the pastette’s content into the bottom of the 2 ml inactivation tube (to minimise bubbling).
?. [Inactivation step]
Reseal the 2 ml inactivation tube.
?. [Inactivation step]
Draw up some  into the pastette.
[Surfanios]
?. [Inactivation step]
Discard the pastette into the liquid waste container.
?. [Inactivation step]
Replace the lid onto the 2 ml inactivation tube.
?. [Inactivation step]
When a rack of inactivated samples is ready to be removed from the MBSC proceed to the 'Removal ofracks of inactivated samples from the MBSC' section below.
?. [Inactivation step]
When a rack of inactivated samples is ready to be removed from the MBSC proceed to the 'Removal ofracks of inactivated samples from the MBSC' section below.
?. [Inactivation step]
Remove over-sleeves and outer gloves.
?. [Inactivation step]
Set the timer outside the MBSC for 10 minutes.
?. [Inactivation step]
Outside the MBSC put on a fresh pair of outer gloves.
?. [Inactivation step]
Outside the MBSC put on a fresh pair of over-sleeves.
?. [Inactivation step]
Unscrew barcoded 2 ml inactivation tube.
Working with multiple samples might lead to errors in sample identification➢ Work with only one swab sample at a time➢ Continue with the protocol ONLY after the 10-minute timer has sounded
?. [Inactivation step]
Place 2 ml inactivation tube lid down to the side.
?. [Inactivation step]
Pick up the corresponding swab container.
?. [Inactivation step]
Check the barcodes on the swab container and opened inactivation tube match.
Correct barcode check➢ This is a vital check to ensure the right sample is processed back into its correspondinginactivation tube to avoid misdiagnosis of patient samples
?. [Inactivation step]
Once the match is verified, unscrew the swab container lid.
?. [Inactivation step]
Discard the swab container lid into the 10% Surfanios liquid waste tub.
?. [Inactivation step]
Holding the swab vial, place hands over the liquid waste container so the swab vial opening is slightly hanging above the liquid waste container to catch any drips.
Take care to not place the whole swab vial directly over the waste container in case it falls/slips/dropped accidentally.
?. [Inactivation step]
Draw up the lysis buffer from the swab container into a fresh pastette.
Important information: the dry swabs will have absorbed some of the lysis buffer➢ Do not expect to recover the full 1 ml of lysis buffer
?. [Inactivation step]
Dispose of the swab container into the 10% Surfanios liquid waste tub.
?. [Inactivation step]
Dispense the pastette’s content into the bottom of the 2 ml inactivation tube (to minimise bubbling).
?. [Inactivation step]
Reseal the 2 ml inactivation tube.
?. [Inactivation step]
Draw up some  into the pastette.
[Surfanios]
?. [Inactivation step]
Discard the pastette into the liquid waste container.
?. [Inactivation step]
Replace the lid onto the 2 ml inactivation tube.
?. [Inactivation step]
When a rack of inactivated samples is ready to be removed from the MBSC proceed to the 'Removal ofracks of inactivated samples from the MBSC' section below.
?. [Removal of racks of inactivated samples from the MBSC]
Visually inspect all the inactivation vials to ensure that all vials are capped.
?. [Removal of racks of inactivated samples from the MBSC]
Place lid loosely on liquid waste container. Dispose of all bags, barcodes and blue roll into the blue waste bag.
?. [Removal of racks of inactivated samples from the MBSC]
Set up a clean area large enough for rack to sit on top of for decontamination, either: a. Spray Amphospray onto tissue and wipe areab. Directly spray an area on the floor of the hood
?. [Removal of racks of inactivated samples from the MBSC]
Spray the rack thoroughly with Amphospray and place on pre-cleaned area in MBSC.
?. [Removal of racks of inactivated samples from the MBSC]
Remove existing “dirty” second gloves and over sleeves. Discard at side of MBSC.
?. [Removal of racks of inactivated samples from the MBSC]
Start 5-minute timer.
?. [Removal of racks of inactivated samples from the MBSC]
When timer rings, remove the rack from the MBSC with clean orange or blue gloves.
?. [Removal of racks of inactivated samples from the MBSC]
Transfer completed inactivation vials to transport rack.
?. [Removal of racks of inactivated samples from the MBSC]
If processing another set of samples, start again by collecting a fresh tub from the corridor.
To prevent fatigue and operator error, work only in pre-arranged 1-hour shifts. If you are in themiddle of a batch, stop and hand over to the next person.
?. [Removal of racks of inactivated samples from the MBSC]
Once full, notify runner that samples are ready for collection.
Batching of tubes for downstream automation processingThe optimal number for plate transfer is 93 tubes➢ Fill each blue lid rack with 93 inactivated samples➢ The final three spaces are required for one positive and two negative controls (added during later steps).
?. [Removal of racks of inactivated samples from the MBSC]
Close box.
?. [Removal of racks of inactivated samples from the MBSC]
Spray box and transfer to corridor.
?. [Removal of racks of inactivated samples from the MBSC]
Place a [removable] tape label on the box.
?. [Removal of racks of inactivated samples from the MBSC]
Write the date and time and room on the box.
?. [Removal of racks of inactivated samples from the MBSC]
Place the rack in the transfer box and replace the lid.
?. [Removal of racks of inactivated samples from the MBSC]
Notify runners that samples are ready.
?. [Removal of racks of inactivated samples from the MBSC]
Restart sample processing.
Once a box is full to the required number of racksa) Place a “outgoing” laminated sign on the transfer boxb) Spray and wipe the transfer box and remove it to the table in the corridorc) Phone sample reception area to inform SHS there is a box ready for collection.
?. [Removal of racks of inactivated samples from the MBSC]
Visually inspect all the inactivation vials to ensure that all vials are capped.
?. [Removal of racks of inactivated samples from the MBSC]
Place lid loosely on liquid waste container. Dispose of all bags, barcodes and blue roll into the blue waste bag.
?. [Removal of racks of inactivated samples from the MBSC]
Set up a clean area large enough for rack to sit on top of for decontamination, either: a. Spray Amphospray onto tissue and wipe areab. Directly spray an area on the floor of the hood
?. [Removal of racks of inactivated samples from the MBSC]
Spray the rack thoroughly with Amphospray and place on pre-cleaned area in MBSC.
?. [Removal of racks of inactivated samples from the MBSC]
Remove existing “dirty” second gloves and over sleeves. Discard at side of MBSC.
?. [Removal of racks of inactivated samples from the MBSC]
Start 5-minute timer.
?. [Removal of racks of inactivated samples from the MBSC]
When timer rings, remove the rack from the MBSC with clean orange or blue gloves.
?. [Removal of racks of inactivated samples from the MBSC]
Transfer completed inactivation vials to transport rack.
?. [Removal of racks of inactivated samples from the MBSC]
If processing another set of samples, start again by collecting a fresh tub from the corridor.
To prevent fatigue and operator error, work only in pre-arranged 1-hour shifts. If you are in themiddle of a batch, stop and hand over to the next person.
?. [Removal of racks of inactivated samples from the MBSC]
Once full, notify runner that samples are ready for collection.
Batching of tubes for downstream automation processingThe optimal number for plate transfer is 93 tubes➢ Fill each blue lid rack with 93 inactivated samples➢ The final three spaces are required for one positive and two negative controls (added during later steps).
?. [Removal of racks of inactivated samples from the MBSC]
Close box.
?. [Removal of racks of inactivated samples from the MBSC]
Spray box and transfer to corridor.
?. [Removal of racks of inactivated samples from the MBSC]
Place a [removable] tape label on the box.
?. [Removal of racks of inactivated samples from the MBSC]
Write the date and time and room on the box.
?. [Removal of racks of inactivated samples from the MBSC]
Place the rack in the transfer box and replace the lid.
?. [Removal of racks of inactivated samples from the MBSC]
Notify runners that samples are ready.
?. [Removal of racks of inactivated samples from the MBSC]
Restart sample processing.
Once a box is full to the required number of racksa) Place a “outgoing” laminated sign on the transfer boxb) Spray and wipe the transfer box and remove it to the table in the corridorc) Phone sample reception area to inform SHS there is a box ready for collection.
?. [Removal of racks of inactivated samples from the MBSC]
Visually inspect all the inactivation vials to ensure that all vials are capped.
?. [Removal of racks of inactivated samples from the MBSC]
Place lid loosely on liquid waste container. Dispose of all bags, barcodes and blue roll into the blue waste bag.
?. [Removal of racks of inactivated samples from the MBSC]
Set up a clean area large enough for rack to sit on top of for decontamination, either: a. Spray Amphospray onto tissue and wipe areab. Directly spray an area on the floor of the hood
?. [Removal of racks of inactivated samples from the MBSC]
Spray the rack thoroughly with Amphospray and place on pre-cleaned area in MBSC.
?. [Removal of racks of inactivated samples from the MBSC]
Remove existing “dirty” second gloves and over sleeves. Discard at side of MBSC.
?. [Removal of racks of inactivated samples from the MBSC]
Start 5-minute timer.
?. [Removal of racks of inactivated samples from the MBSC]
When timer rings, remove the rack from the MBSC with clean orange or blue gloves.
?. [Removal of racks of inactivated samples from the MBSC]
Transfer completed inactivation vials to transport rack.
?. [Removal of racks of inactivated samples from the MBSC]
If processing another set of samples, start again by collecting a fresh tub from the corridor.
To prevent fatigue and operator error, work only in pre-arranged 1-hour shifts. If you are in themiddle of a batch, stop and hand over to the next person.
?. [Removal of racks of inactivated samples from the MBSC]
Once full, notify runner that samples are ready for collection.
Batching of tubes for downstream automation processingThe optimal number for plate transfer is 93 tubes➢ Fill each blue lid rack with 93 inactivated samples➢ The final three spaces are required for one positive and two negative controls (added during later steps).
?. [Removal of racks of inactivated samples from the MBSC]
Close box.
?. [Removal of racks of inactivated samples from the MBSC]
Spray box and transfer to corridor.
?. [Removal of racks of inactivated samples from the MBSC]
Place a [removable] tape label on the box.
?. [Removal of racks of inactivated samples from the MBSC]
Write the date and time and room on the box.
?. [Removal of racks of inactivated samples from the MBSC]
Place the rack in the transfer box and replace the lid.
?. [Removal of racks of inactivated samples from the MBSC]
Notify runners that samples are ready.
?. [Removal of racks of inactivated samples from the MBSC]
Restart sample processing.
Once a box is full to the required number of racksa) Place a “outgoing” laminated sign on the transfer boxb) Spray and wipe the transfer box and remove it to the table in the corridorc) Phone sample reception area to inform SHS there is a box ready for collection.
?. [Movement of full transfer boxes to RNA extraction lab]
Runners' tasks entering CL3 facility:
Full boxes of inactivated samples will be transported from the CL2/3 to RNA extraction lab byHealth & Safety or other nominated staff (runners).
?. [Movement of full transfer boxes to RNA extraction lab]
Put on a pair of gloves in the gowning room.
?. [Movement of full transfer boxes to RNA extraction lab]
Enter the inner corridor.
?. [Movement of full transfer boxes to RNA extraction lab]
Put on a purple lab coat.
?. [Movement of full transfer boxes to RNA extraction lab]
Go to the relevant room.
?. [Movement of full transfer boxes to RNA extraction lab]
Spray the outside of the transfer box with Amphospray.
?. [Movement of full transfer boxes to RNA extraction lab]
Wipe down the outside of the box with tissue.
?. [Movement of full transfer boxes to RNA extraction lab]
Discard the tissue into the waste bin in the room.
?. [Movement of full transfer boxes to RNA extraction lab]
Spray your gloves with Amphospray.
?. [Movement of full transfer boxes to RNA extraction lab]
Leave the room with the transfer box.
?. [Movement of full transfer boxes to RNA extraction lab]
Place the transfer box on the table by the exit.
?. [Movement of full transfer boxes to RNA extraction lab]
Remove and hang up your lab coat.
?. [Movement of full transfer boxes to RNA extraction lab]
Remove your gloves.
?. [Movement of full transfer boxes to RNA extraction lab]
Dispose of the gloves in the blue autoclave bin under the table.
?. [Movement of full transfer boxes to RNA extraction lab]
Take the transfer box into gowning room.
?. [Movement of full transfer boxes to RNA extraction lab]
Wash your hands.
?. [Movement of full transfer boxes to RNA extraction lab]
Leave the gowning room.
?. [Movement of full transfer boxes to RNA extraction lab]
Deposit the samples at the RNA extraction lab reception area.
?. [Movement of full transfer boxes to the ASF on L05]
Runners' tasks entering CL3 area:
Full boxes of inactivated samples will be transported from the CL2/3 to RNA extraction lab byHealth & Safety or other nominated staff (runners).
?. [Movement of full transfer boxes to the ASF on L05]
Put on a pair of gloves in the gowning room.
?. [Movement of full transfer boxes to the ASF on L05]
Enter the inner corridor.
?. [Movement of full transfer boxes to the ASF on L05]
Put on a purple lab coat.
?. [Movement of full transfer boxes to the ASF on L05]
Go to the relevant room.
?. [Movement of full transfer boxes to the ASF on L05]
Spray the outside of the transfer box with Amphospray.
?. [Movement of full transfer boxes to the ASF on L05]
Wipe down the outside of the box with tissue.
?. [Movement of full transfer boxes to the ASF on L05]
Discard the tissue into the waste bin in the room.
?. [Movement of full transfer boxes to the ASF on L05]
Spray your gloves with Amphospray.
?. [Movement of full transfer boxes to the ASF on L05]
Leave the room with the transfer box.
?. [Movement of full transfer boxes to the ASF on L05]
Place the transfer box on the table by the exit.
?. [Movement of full transfer boxes to the ASF on L05]
Remove and hang up your lab coat.
?. [Movement of full transfer boxes to the ASF on L05]
Remove your gloves.
?. [Movement of full transfer boxes to the ASF on L05]
Dispose of the gloves in the blue autoclave bin under the table.
?. [Movement of full transfer boxes to the ASF on L05]
Take the transfer box into gowning room.
?. [Movement of full transfer boxes to the ASF on L05]
Wash your hands.
?. [Movement of full transfer boxes to the ASF on L05]
Leave the gowning room.
?. [Movement of full transfer boxes to the ASF on L05]
Deposit the samples at the RNA extraction lab reception area.
?. [Movement of full transfer boxes to the ASF on L05]
Runners' tasks entering CL3 facility:
Full boxes of inactivated samples will be transported from the CL2/3 to RNA extraction lab byHealth & Safety or other nominated staff (runners).
?. [Movement of full transfer boxes to the ASF on L05]
Put on a pair of gloves in the gowning room.
?. [Movement of full transfer boxes to the ASF on L05]
Enter the inner corridor.
?. [Movement of full transfer boxes to the ASF on L05]
Put on a purple lab coat.
?. [Movement of full transfer boxes to the ASF on L05]
Go to the relevant room.
?. [Movement of full transfer boxes to the ASF on L05]
Spray the outside of the transfer box with Amphospray.
?. [Movement of full transfer boxes to the ASF on L05]
Wipe down the outside of the box with tissue.
?. [Movement of full transfer boxes to the ASF on L05]
Discard the tissue into the waste bin in the room.
?. [Movement of full transfer boxes to the ASF on L05]
Spray your gloves with Amphospray.
?. [Movement of full transfer boxes to the ASF on L05]
Leave the room with the transfer box.
?. [Movement of full transfer boxes to the ASF on L05]
Place the transfer box on the table by the exit.
?. [Movement of full transfer boxes to the ASF on L05]
Remove and hang up your lab coat.
?. [Movement of full transfer boxes to the ASF on L05]
Remove your gloves.
?. [Movement of full transfer boxes to the ASF on L05]
Dispose of the gloves in the blue autoclave bin under the table.
?. [Movement of full transfer boxes to the ASF on L05]
Take the transfer box into gowning room.
?. [Movement of full transfer boxes to the ASF on L05]
Wash your hands.
?. [Movement of full transfer boxes to the ASF on L05]
Leave the gowning room.
?. [Movement of full transfer boxes to the ASF on L05]
Deposit the samples at the RNA extraction lab reception area.