eamag/protocols-with-steps huggingface dataset:
TITLE: iPSC PCR: For Screening Edited Clones
AUTHORS: Celeste Karch, Rita Martinez, Jacob Marsh

[STEPS]
?. [PCR]
This is the set up for a 50 µL reaction.  ABC1Volumex# rxns25x Green GoTaq Flexi Buffer10 µl325mM MgCl26 µl425mM dNTPs0.8 µl5Forward Primer (10uM)2 µl6Reverse Primer (10uM)2 µl7GoTaq DNA Polymerase (5U/uL)0.25 µl8Milli-Q H2026.95 µl9Quick Extract gDNA2 µl10Total50 µl
ABC1Volumex# rxns25x Green GoTaq Flexi Buffer10 µl325mM MgCl26 µl425mM dNTPs0.8 µl5Forward Primer (10uM)2 µl6Reverse Primer (10uM)2 µl7GoTaq DNA Polymerase (5U/uL)0.25 µl8Milli-Q H2026.95 µl9Quick Extract gDNA2 µl10Total50 µl
?. [PCR]
After PCR has been set up, run in thermocycler using the following conditions.  ABCD1SegmentCyclesTemperatureTime21195˚C2 minutes324095˚C30 seconds465˚C - 1˚C/cycle30 seconds572˚C1 minute63172˚C5 minutes7414˚CForever
ABCD1SegmentCyclesTemperatureTime21195˚C2 minutes324095˚C30 seconds465˚C - 1˚C/cycle30 seconds572˚C1 minute63172˚C5 minutes7414˚CForever
?. [PCR]
After PCR has been run in thermocycler, it is best to run the sample on a 2% gel to confirm the presence of PCR product.
?. [Gel preparation]
Pour gel from flask into casting tray (be sure to add appropriate amount of combs to casting tray).
?. [Gel preparation]
Let sit for 30-40 minutes, or until firm.
?. [Gel preparation]
Place gel cast into the gel rig apparatus.
?. [Gel preparation]
Load samples.
Be sure to include positive and negative controls when possible.
?. [Gel preparation]
To make the gel, combine an appropriate amount of Agarose, TBE and Ethidium Bromide using the following guidelines.a. The 2% gel will be cast in one of the following ways:  ABC115x15 cast15x25 cast2Agarose1.5 g3.0 g3TBE75 mL150 mL4Ethidium Bromide3.75 uL7.5 uLb. Combine Agarose and TBE in an appropriately sized flask and microwave until Agarose is completely dissolved. Swirl intermittently during heating.c. Once completely dissolved add appropriate amount of Ethidium Bromide to flask and swirl until dispersed evenly.
ABC115x15 cast15x25 cast2Agarose1.5 g3.0 g3TBE75 mL150 mL4Ethidium Bromide3.75 uL7.5 uL
?. [Gel preparation]
Load 50 bp ladder.
?. [Gel preparation]
Place lid on gel rig apparatus.
?. [Gel preparation]
Run gel at 150 volts for  (checking at  to ensure samples have not run too far or off the gel).
?. [Gel preparation]
Turn off gel rig apparatus and remove cast.
?. [Gel preparation]
Blot off excess TBE from cast.
?. [PCR]
?. [Gel preparation]
Visualize gel
?. [Gel preparation]
If PCR product is the approrpiate size and concentration as indicated by the gel, use the remaining PCR product to perform restriction digest
TITLE: SOP for genome size estimations from collection specimens
AUTHORS: Jean François Flot, Mohammed Tawfeeq, Ana Riesgo

[DESCRIPTION]
Genome size data is crucial now for comparative studies with the goal of understanding the mechanisms of genomic change and their biological implications related to genome size diversity. But also, now this data is actively sought by the
biodiversity genomics community given that is a a valuable proxy for the estimations of the cost and challenge of sequencing and assembling a potential target genome. Given the (sometimes) extreme diversity of genome sizes among the organisms within the same phyla or even genus, it is important to provide genome size estimations for the maximum number of taxa possible, to aid on the accuracy of genome sequencing depth, among other things. Traditionally, genome size estimations for multicellular species are performed using two methods: Feulgen densitometry (now most often based on image analysis) and flow cytometry. In both cases, live/fresh or flash-frozen (e.g., in liquid nitrogen or on dry ice) material of animal tissues are preferred over fixed material because the nuclei remain intact and can be prepared and stained more easily. However, this poses many challenges and limitations for rare taxa and those coming from extreme environments or sites that are very difficult to access. Therefore, estimations from preserved material are now more necessary than ever if we want to accomplish the difficult task of sequencing virtually the whole biodiversity of the planet. 

[STEPS]
TITLE: Growth rate determination of E. coli
AUTHORS: Ashwinuday 

[DESCRIPTION]
<div class = "text-blocks"><br/><div class = "text-block"><span>This is to measure the growth rate of </span><span style = "font-style:italic;">E. coli.</span></div><br/><div class = "text-block">The initially used culture is a primary culture. </div><br/><div class = "text-block">This can be adapted for any other bacteria as well by giveing proper growth conditions, and using appropriate wavelength to measure the OD and giving proper time intervals based on whether the bacteria is slow growing or fast growing.</div></div>

[STEPS]
?. Grow the bacteria in an overnight (14-18 hours) culture.
?. Inoculate 50 ml autoclaved culture media in a conical flask with a part of the overnight grown culture equal to 2% volume of fresh media (i.e. 1ml) inside a laminar hood.
?. Put the rest of the freshly inoculated flask into a shaking incubator at   at .
33
37 °C
?. Mix the contents well and take 1 ml of the media into a clean cuvette and measure its OD at 600nm in a spectrophotometer.This will serve as the 0 hour reading.
?. At every 1 hour take out the flask, open inside a hood, mix well,  quickly take 1 ml of the culture into a cleaned cuvette and again measure its OD at 600nm.
If doing alone, keep the clean cuvette in ice and transfer the culture into that cuvette to prevent any more growth to occur (Especially in log phase) during the time of handling the main culture.
?. Note down the OD till 20 hours. Then plot a graph of OD v/s time.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>prepare 100 mL per 250 mL flask for each overnight culture</p>
<p>for     </p>
<h1>CZV8CS - liquid media for flask culture</h1>
<p>prepare 100 mL per 250 mL flask for each overnight culture for</p>
<p>                                                   <strong><u>100 mL</u></strong>:                                <strong><u>300 mL</u></strong></p>
<p>Czapek-Dox liquid medium         4.54 g                                  13.62 g        </p>
<p>centrifuged V8 juice                    20 mL                                   60 mL</p>
<p>add H<sub>2</sub>0 to                                  95 mL                                   285 mL</p>
<p>        <br />adjust to pH 6.0 transfer to</p>
<p>transfer to flask(s) and autoclave</p>
<p>add 5 mL sterile complete supplement to media after autoclaving</p>
<p> </p>
<p><strong><em>Complete Supplement (CS)</em></strong></p>
<p>Bacto-casamino acids     20 g/l</p>
<p>Bacto-peptone                 20 g/l</p>
<p>Bacto-yeast extract         20g/l</p>
<p>Adenine                           3 g/l <strong><sup>9</sup></strong></p>
<p>Biotin                               0.02 g/l</p>
<p>Nicotinic acid                   0.02 g/l</p>
<p>P-aminobenzoic acid       0.02 g/l <strong><sup>10</sup></strong></p>
<p>Pyridoxine                        0.02 g/l</p>
<p>Thiamine                         0.02 g/l</p>

[STEPS]
?.
TITLE: Permanent multiprofessional auditory assessment protocol for children with congenital zika virus syndrome
AUTHORS: Klinger Vagner Teixeira da Costa, Pedro Menezes, Ana Frizzo

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Trata-se de 0um protocolo de avaliação auditiva multiprofissional para crianças com microcefalia na síndrome congênita do vírus da zica. Este protocolo contempla a avaliação clinica por neuropediatra, otorrinolaringologista e fonoaudiólogo de forma simultânea. A seguir, a criança é avaliada por exames eletrofisiológicos para pesquisa de perdas auditivas tanto periféricas como centrais.</div></div>

[STEPS]
TITLE: Calcium fluorimetry with the FLIPR Calcium 6 kit on FlexStation 3
AUTHORS: Angus Li

[DESCRIPTION]
This protocol details an experimental procedure used to generate results described in the manuscript Li, A., Liu, S., Huang, R., Ahn, S., & Lefkowitz, R. J. (2023). Loss of biased signaling at a G protein-coupled receptor in overexpressed systems.

[STEPS]
1. Plate U2OS-TetOn-AT1R in microplates (Black with clear bottom, lysine- coated Corning 3842) at 15000 cells/well 2 days prior to assay
2. Add doxycycline and optionally PTX to wells 14 hours before replacement with loading buffer
3. Remove one vial of Calcium 6 Assay Reagent (Component A, good for 2 plates) from the freezer and equilibrate to room temperature.
4. Dissolve contents of one Component A vial by adding 10 ml of 1X HBSS Buffer plus 20 mM HEPES. Mix by vortexing (~1-2 min) until contents of vial are dissolved. It is important that contents are completely dissolved to ensure reproducibility between experiments.
5. Remove cell plates from the incubator, Calcium 6 Kit - add an equal volume of loading buffer to each well in the dark (i.e. 120ul HBSS+HEPES + 40ul of Dye/well (4X) for a 96-well plate in the original protocol). Use 11.8ml of HBSS+HEPES +4.8ml of Dye (4X) for one plate. Save the dissolved dye solution in -20.
6. Return plates to the incubator and incubate two hours at 37 C, 5% CO2
7. Optionally, add YM-254890 1 hour prior to reading
8. 30 minutes before experiment (1.5 hours into incubation), prepare the reagents (5X, 40ul/well+20ul volume). The ligand needs to be plated in a 96 well with round bottom (The FlexStation will add ligand, shake, and measure sequentially).
9. Turn on FlexStation 30 minutes before experiment and warm up to 37.
10. Place tips and ligand plate
11. After the two-hour incubation period, transfer the assay plate directly to the FlexStation instrument assay plate carriage 10 minutes prior to reading to re-equilibrate temperature, and then run the assay.
12. In an individual well or column of wells, the calcium flux peak(s) should be complete within 1 to 3 minutes after addition. For an entire plate however, the protocol will not complete until all chosen columns are finished. 40-45 min for entire 96 well plate.
TITLE: Chemotaxonomy-based Drug Discovery
AUTHORS: contact.microbialtec  

[DESCRIPTION]
With the large numbers of already known microbial and plant metabolites, one of the major challenges in modern natural product discovery is to detect already known and trivial compounds rapidly. This relies on information management and data mining of the enormous amounts of biological, chromatographic and spectroscopic data generated in modern drug discovery. To optimize the drug discovery process, the cultures of a given microbial collection (culture collection or natural samples) are selected for extraction and biological evaluation. Microbial strains have been selected based on morphology, and with more powerful approaches such as automated image analysis of pure cultures, phenotypic characters including production of secondary metabolite profiles or based on genotyping.

[STEPS]
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Adapted from Tara Oceans extraction protocol (see citation).</p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?. 
?.
TITLE: Make NGM medium
AUTHORS: Cancer Research UK / Wellcome Trust Gurdon Institute media kitchen

[STEPS]
?. 
?. Add bacto peptone and NaCl
?. Measure approx 4.8L  double distilled H2O in 5L bell jar with a magnetic flea.
?. Stir until all solutes are dissolved.
?. Dispense approx 972ml  NGM media per 1L bottle OR 486ml NGM into a 500ml bottle.
?. Dispense 17g of agar and a magnetic flea to each 1L bottle OR 8.5g of agar and a magnetic flea to each 500ml bottle.
?. Label, date and autoclave.
?. ABC1NaCl 15g2bacto
  peptone12.5g3Double
  distilled H2O4.86L4Agar&nbsp;&nbsp;per 1L bottle17g
ABC1NaCl 15g2bacto
  peptone12.5g3Double
  distilled H2O4.86L4Agar&nbsp;&nbsp;per 1L bottle17g
TITLE: PNA/DNA Chimera Synthesis
AUTHORS: Cathy  Miller

[DESCRIPTION]
Pure PNA has significant binding properties, but it has completely different properties from DNA and RNA. For example, it cannot be recognized as a substrate when it interacts with nucleic acid modifying enzymes. On the one hand, this is an advantage, because it makes PNA very stable for the degradation of nucleases; but on the other hand, it also limits certain applications of PNA, such as not being used as a substrate for DNA polymerase, DNA kinase or DNA ligase. In one molecule, the combination of PNA and DNA can produce PNA/DNA chimeras with new properties. This substance significantly improves the solubility of PNA in aqueous solutions, PNA/DNA chimera can be analyzed and purified by PAGE and ion exchange chromatography because the DNA part is negatively charged. Interestingly, PNA/DNA chimeras also have biological functions, such as being used as primers for DNA polymerases or binding to RNA to mediate the dissociation of RNase H. Finally, the study of PNA/DNA chimeras can provide useful information about the structural and functional properties of DNA and RNA themselves.

Creative Peptides provides PNA/DNA chimera related services, we can provide design and synthesis integrated services, and committed to helping you solve synthesis problems and customize high-stability, multi-type PNA/DNA chimera products.

[STEPS]
TITLE: Minimum Inhibitory Concentration
AUTHORS: Amanda T Alker, Nicholas J Shikuma

[DESCRIPTION]
Protocol for determining the minimum inhibitory concentration of antibiotics for marine bacteria.

[STEPS]
SECTION: Day 1
1. Streak out marine microbes onto Marine Agar plates and incubate overnight at 25-28ºC. 

 

SECTION: Day 2
2. Inoculate a single colony into 5mL Marine Broth (2216) media in the late afternoon/ early evening. Incubate overnight at 25ºC, shaking at 200 rpm. 

SECTION: Day 3
3. Measure optical density (OD600) of overnight culture growth and document

SECTION: Day 3
4. Pipette 100µL of overnight culture from each strain onto Marine Agar plates containing either Strep (for background resistance), Kan or Gent (MIC) at each concentration (25µg/mL, 50µg/mL, 100 µg/mL and 200µg/mL). 


SECTION: Day 3
5. Use beads or plate spreader to spread the culture evenly on the plate. Remove the beads and incubate overnight at 25ºC overnight. 
SECTION: Day 4
6. Observe and document growth on all concentration plates. 
For the MICs (Kan/Gent) identify the lowest concentration of media in which no colonies are observed. 

 

SECTION: Day 5
8. Observe and document growth on both the MIC plates (2 days old) and the Streptomycin resistance plates. If Streptomycin resistance is sustained at less than 200µg/mL, then continue step 7, passaging the strains onto higher concentrations of antibiotics. Once robust growth occurs at 200µg/mL, inoculate a single colony into 5mL Marine Broth containing Strep 200µg/mL and incubate 25ºC overnight, shaking at 200rpm. 
SECTION: Day 4
7. For the Streptomycin Resistant strain, select a single colony from the highest dose of Streptomycin that has growth and streak it out onto a plate containing the same concentration AND a plate containing the next highest concentration of antibiotics. Incubate overnight at 25C. 
SECTION: Day 6 or later
9. Store culture in cryovials for long term storage. Add 500µL overnight culture to 500µL 50% glycerol, pipette mix and store in the -80C for future use.
TITLE:  Deparaffinization for tissue and organoids 
AUTHORS: Gabriela Vallejo Flores, Annika Fendler

[DESCRIPTION]
This protocol is to withdraw the paraffin from formalin-fixed paraffin-embedded tissue and organoids and to recover the protein structure of your sample. 

[BEFORE_START]
Prepare the steamer and the cook heater for boiling the samples into the antigen retrieval solution
Fill the Glass bucket with the solutions on step 1


[GUIDELINES]
Before star the experiment prepare the following solutions;
TBS 1x
Sodium citrate buffer (10 mM Sodium citrate, 0.05% Tween 20, pH 6.0)
Tri-sodium citrate (dihydrate) 2.94 g
Distilled water 1 L
Mix to dissolve. Adjust pH to 6.0 with 1N HCl
Add 0.5 mL Tween 20 and mix well. Store at room temperature for 3 months or at
4°C for longer storage

Tris-EDTA buffer (10 mM Tris base, 1 mM EDTA solution, 0.05% Tween 20, pH 9.0)
Tris 1.21 g
EDTA 0.37 g
Distilled water 1 L
Mix to dissolve. Adjust pH to 9.0.
Add 0.5 mL of Tween 20 and mix well. 
Store at room temperature for 3 months or at 4°C for longer storage
96% ETOH
80% ETOH
70% ETOH



[STEPS]
SECTION: Antigen retrieval solution
2. Transfer the slide into the antigen retrieval solution in the steamer, turn on the cook heater and incubate 5 min after the valve is up, turn off the cook heater and wait till the valve get down again.     


SECTION: Antigen retrieval solution
4. Transfer the slides into destillated water, now the slides are ready for immunostaining. 
SECTION: Deparaffinization
1. 
 

SECTION: Antigen retrieval solution
3. Inmmerse the steamer in running water for 5 min to help the steamer get cooler,  open the steamer and add running water to the slides during one min. 
SECTION: Antigen retrieval solution
5.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This is a variation of the CagA translocation assay performed in our lab.</p>
<p>This protocol has been used in the publication DOI: <a href="https://doi.org/10.1111/cmi.12166" target="_blank">10.1111/cmi.12166</a> </p>
<p> </p>

[STEPS]
?. 
?. 
?. 
?. 
?. 
?. 
?. 
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?.
TITLE: 10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Multiplex -- University of Minnesota TMCs (CG000527 Rev E)
AUTHORS: IOx Genomics, Laura Niedernhofer, David A Bernlohr

[DESCRIPTION]
10x Genomics Chromium Single Cell Expression flex protocol for library construction. 

Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment. 

[STEPS]
SECTION: Preparation
1. Complete single cell or nuclei isolation and 10x fixation prior to starting this protocol
SECTION: Library Preparation
2.   
 

SECTION: FASTQ Generation
3. BCL data from Illumina sequencer is demultiplexed and converted into FASTQ format using bcl2fastq version 2.20.0
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Counting cells with a hemocytometer is an easy way to determine relatively accurate numbers of viable cells.  After determining cell counts, cells can be passaged, frozen away, or used for an experiment at a particular density.  </p>

[GUIDELINES]
<p>Keep cells sterile by wearing gloves, ethanol treating your hands and surfaces, using sterile pipette tips, and <strong><em><u>only opening vials with cells in the BSL-2 laminar flow hood</u></em></strong>.</p>

[STEPS]
?. 
?. 
?. 
?. 
?.
TITLE: UPitt TriState SenNet TMC Cryopreserved Tissue Grinding
AUTHORS: Marta Bueno

[DESCRIPTION]
This document outlines the tissue pulverization protocol of lung and heart specimens at the TriState SenNet TMC Biospecimen Core at the University of Pittsburgh, as part of the Cellular Senescence Network Program (SenNet).

[BEFORE_START]
Use clean and/or autoclaved tools.
Use RNAZap over all sufaces and be aware of cross-contamination sources.

[GUIDELINES]
Ensure the samples are kept frozen on dry ice throughout pulverization.

[STEPS]
SECTION: Tissue pulverization
1. Pour liquid nitrogen into a mortar and pestle until frozen. 

SECTION: Tissue pulverization
2. Remove tissue from tube and place into liquid nitrogen with a clean cold spatula (precool in liquid
nitrogen).
SECTION: Tissue pulverization
3. Grind up the sample with liquid nitrogen using the mortar and pestle. 
SECTION: Tissue pulverization
4. Use a clean cold spatula to scoop ground tissue to 1.5 mL LoBind tube (Eppendorf). 
SECTION: Tissue pulverization
5. Place the sample back onto dry ice or directly to -80 °C. 
SECTION: Tissue pulverization
6. Clean the mortar and pestle with 10% bleach and 70% ethanol before using it for the next sample.
TITLE: Co-existing OSA reduce Nuss surgery efficacy
AUTHORS: Mei-Chen Yang

[DESCRIPTION]
Nuss surgery is effective for pectus excavatum (PE), with a recurrence rate of 1.2–27%. Re-do surgery is effective, but still has a 6% failure rate. Patients with obstructive sleep apnea (OSA) experience repetitive PE-associated sternal depression during sleep. As the prevalence of OSA among PE patients is higher than the average, co-existing OSA in PE patients might negatively impact the efficacy of Nuss surgery. This study aimed to evaluate the impact of co-existing OSA on Nuss surgery in patients with PE.Totally, twenty adult patients with PEonly and nine patients with PE and OSAwere analyzed. Polysomnography was performed before Nuss surgery to evaluate OSA. Sternovertebral distance (SVD) and radiographic Haller index (RHI) were recorded before surgery and at 3, 6, and 24 months postoperatively. The results showed that, post-operatively, percentage changes in SVD in patients with PE only at 3, 6, and 24 months postoperatively were significantly increased compared with those in thepatients with PE and OSA (31.1% vs. 14.1% at 3 months; 37.5% vs. 21.4% at 6 months; 42.5% vs. 19.2% at 24 months), while percentage changes in RHI were significantly lower in patients with PE only than in the patients with PE and OSA (-22.9% vs. -9.3% at 3 months; -27.9% vs. -18.7% at 6 months; -30.6% vs. -16.7% at 24 months). The study showed that co-existing OSA might reduce the efficacy of Nuss surgeryfor patients with PE. We recommended that patients with PE should be evaluated for OSA before surgery and be promptly treated for OSA until making sure the PE does not recur after bal removal.

[STEPS]
TITLE: Protocols for activity changes in Neuron-Astrocyte Networks in Culture Under the Effect of Norepinephrine
AUTHORS: Yasmin Bar El, Sivan Kanner

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>Protocols for Primary cortical neuronal-astrocyte cell culture, Isolated astrocyte cell culture, Immunocytochemistry, Electrophysiology and Ca</span><span style = "vertical-align:super;">2+ </span><span>imaging.</span></div></div>

[STEPS]
TITLE: Exp_01 Taza
AUTHORS: Mayleth Medina Cárdenas, Estefanía Sauceda Camacho, Sofía Morga Benítez

[DESCRIPTION]
Este experimento consiste en la medición de la temperatura de 250 mL de agua contenida en una taza, desde que el agua se encuentra a una temperatura de 84ºC, hasta llegar a una temperatura ambiente de 22ºC como efecto de la Ley 0 de la Termodinámica. Esto se llevó a cabo a través de sensores de temperatura conectados a un circuito de Arduino, y mediante CoolTerm, fueron capturadas las mediciones de temperatura con respecto al tiempo, cada 6 segundos, para después regristarlas en Excel y Matlab, en donde se graficaron y se obtuvo que el cambio de temperatura sigue un modelo de función exponencial.

[GUIDELINES]
Para su realización, se debe mantener una temperatura ambiente constante en todo momento. Además, asegurarse de las conexiones de los sensores y su correcto funcionamiento previo a la experimentación.

[STEPS]
SECTION: Circuito Arduino y software
1. Armar el siguiente circuito:

 

SECTION: Circuito Arduino y software
2. Conectar el cable USB 2.0 a la placa de Arduino y a la laptop.
SECTION: Experimentación
5. Hervir agua y con esta vaciar por completo una tasa de 250 mL 

SECTION: Resultados de mediciones
8. Las mediciones registradas en el archivo .txt obtenidas mediante CoolTerm se pasarán a un archivo Excel, para crear con ellas una tabla, y posteriormente depurarlas.
SECTION: Circuito Arduino y software
3. En el software de Arduino seleccionar la placa y puertos correspondientes.
SECTION: Experimentación
6. Colocar un sensor de temperatura dentro de la taza, y el otro sensor por fuera de esta, en su asa.
SECTION: Circuito Arduino y software
4. Configurar CoolTerms para registrar la lectura de datos en un archivo .txt. 
SECTION: Experimentación
7. La duración del experimento será hasta que la temperatura del agua alcance el equilibrio térmico con la temperatura ambiente de 22 °C .

SECTION: Circuito Arduino y software
3.1. Subir / cargar los programas en Arduino.
SECTION: Resultados de mediciones
9. Subir el excel de las mediciones a Matlab, y con ellas generar una gráfica del cambio de temperatura con respecto al tiempo.