eamag/protocols-with-steps huggingface dataset:
TITLE: EIT data aquisition in rat sciatic nerve using stimulation of tibial and peroneal branches
AUTHORS: Kirill Aristovich, Matteo Donega, David Holder

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">High-level protocol to obtain the EIT imaging dataset and reconstruct images for the paper.</div></div>

[STEPS]
?. [Hardware]
Prepare nessesary hardware:
?. [Neural Interface]
Prepare neural interface:Follow the instructions in teh paper http://iopscience.iop.org/article/10.1088/1741-2552/aae868
Some of the chemical components are toxicChemical hood cabinet is required
?. [Software]
?. [Animal Preparation]
Sprague-Dawley adult male rats weighing 300 to 450g.
?. [Surgery]
Acess sciatic trunc, common peroneal nerve and tibial nerve following the guide:https://onlinelibrary.wiley.com/doi/full/10.1002/mus.21652
Access to the common peroneal nerve was established through a 2mm lateral incision in biceps femoris near the knee joint.
?. [Electrode placement]
Implant the stimulation electrodes on common peroneal and tibial branches (bipolar Ag hook electrodes)
?. [Data aquisition]
For all studies, a square biphasic (positive first) constant current temporal waveform was delivered using a balanced current source (Keithley, UK model no 6221) with: 1-3 mA amplitude,  50 μs pulse width,  5Hz frequency (200 ms inter-stimulus time)
?. [Data Processing and Image reconstruction]
Load and process the data using the following software:
?. [Animal Preparation]
Anaesthesia is induced with a 5% isoflurane in 100% O2.
?. [Animal Preparation]
ntubated using a small animal laryngoscope
?. [Animal Preparation]
Arterial and venous access was established through cannulation (BD Insyte/Vialon, Becton, Dickinson U.K. Ltd.) of the right femoral vessels.
?. [Animal Preparation]
18G cannula and mechanical ventilation is provided, using a Harvard Apparatus Inspira Ventilator (Harvard Apparatus, Ltd, UK), with a 50/50% gas mixture of O2 and air.
?. [Animal Preparation]
The arterial blood pressure should is monitored (Cardiocap 5, Datex Ohmeda) and the mean arterial pressure (MAP) kept between 90 and 110mmHg using labetalol and adrenaline as necessary.
?. [Animal Preparation]
Once intravenous access had been established, a constant infusion of propofol is initiated. Monitor the depth with pinch reflex.
?. [Animal Preparation]
Procedures were performed on a vibration isolated table (Thorlabs Inc., USA) and, throughout experiments, the core body temperature of the rat was controlled with a homeothermic heating unit (Harvard Apparatus, Kent, UK) and maintained at 37⁰C
?. [Electrode placement]
Implant the EIT array on main sciatic trunc
?. [Data aquisition]
Program the EIT system with the following paerameters:
?. [Data aquisition]
Connect stimulator to the tibial stimulation electrode. Collect the data
?. [Data aquisition]
Connect stimulator to the common peroneal stimulation electrode. Collect the data
?. [Data Processing and Image reconstruction]
Forward solution
?. [Data Processing and Image reconstruction]
Inverse solution and images generation
TITLE: No Title
AUTHORS: 

[GUIDELINES]
This protocol describes conditions using either ABI reagents or PGC Scientific Reagents. The reactions have been tested on the gradient Eppendorf, the Gene Techne and the ABI 9700 thermal cyclers and all machines use the same cycling conditions.<br /><br />When testing plasmid DNA preps, use 0.5 µL of DNA as the template in place of the bacterial colony.<br /><br /><strong>Recommened vector primers:</strong><br /><br />
<table>
<tbody>
<tr>
<td>Primer</td>
<td>Sequence (5' to 3')</td>
<td>Fragment Size</td>
</tr>
<tr>
<td>pUC 18 Forward</td>
<td>TGT AAA ACG ACG GCC AGT</td>
<td rowspan="2">109 bp^12<br /><br /></td>
</tr>
<tr>
<td>pUC 18 Reverse</td>
<td>TCA CAC AGG AAA CAG CTA TGA C</td>
</tr>
</tbody>
</table>
<p><br /><strong>Thermal Cycling Protocol:<br /><br /></strong></p>
<table>
<tbody>
<tr>
<td>95°C</td>
<td>5 minutes</td>
<td>1 cycle</td>
</tr>
<tr>
<td>95°C<br />55°C<br />70°C</td>
<td>30 seconds<br />30 seconds<br />30 seconds*</td>
<td>35 cycles</td>
</tr>
<tr>
<td>70°C</td>
<td>7 minutes</td>
<td>1 cycle</td>
</tr>
<tr>
<td>4-10°C</td>
<td> </td>
<td>Hold cycle</td>
</tr>
</tbody>
</table>
*increase to 1 minute if insert is great than 1 Kb<br /><br /><strong>Reagent catalog numbers</strong><br /><br />ABI Reagents:<br />-  dNTP mix N808-0007<br />-  AmpliTaq Gold N808-0247<br /><br />PGC Scientific Reagents<br />-  dNTP mix 62-6113-40<br />-  Taq DNA polymerase (w/10x Buffer containing MgCl<sub>2</sub>) 62-6086-02<br /><br />In pBlueScript these primers bind at nt 599 and nt 833 = 234 bp amplicon.<br />In pGEM-t Easy the primers bind at nt 2975 and nt 197 = 242 bp amplicon.

[STEPS]
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TITLE: 1. RNA isolation for tissue
AUTHORS: Sze-Xian Lim, Chin Yee Tan

[STEPS]
?. Homogenization in TriZolAdd 1mL of Trizol reagent per 30mg of tissue and homogenize using handheld homogenizer
?. Incubate at RT for 5 mins to allow nucleoprotein complexes to dissociate
?. Spin down at max speed, chilled centrifuge for 15 minutes
?. Carefully remove the top aqueous phase and transfer to a new Eppendorf tubeThe interphase and bottom organic phase can be saved for DNA and protein respectively
?. To the aqueous phase, add 500 microliters of 100% isopropanol, mix by inversion and incubate at -20C for a minimum for 2hrs
?. Spin down at max speed for 30 minutes to precipitate RNA
?. Remove supernatant, and add 1mL of 75% Ethanol to wash the pellet
?. Add 1/5 the volume of trizol of chloroform carefully, and vortex to mix well
?. Spin down at max speed for 15 minutes and remove supernatant
?. Resuspend pellet in appropriate volume of nuclease free water
TITLE: Post-Surgical Dissection of Ovaries
AUTHORS: Stephen Fisher, Marielena Grijalva, Rong Guo, sarahjoh, Hieu Nguyen, John Renz, Jean G Rosario, Steven Rudich, Brian Gregory, Junhyong Kim, Kate O'Neill

[DESCRIPTION]
This protocol details dissection of the ovaries in preparation for 10X Visium, 10X Multiomics, pathology review, and biobanking. Care must be taken to systematically divide the ovary and to maintain orientation of the specimens as they are removed using the three points of fixation for the ovary (the uteroovarian ligament, the infundibulopelvic ligament and the mesosalpinx). Once the ovary is isolated it is difficult to discern which aspect of the ovary is superior to the ovary's attachment to the mesosalpinx and which is inferior. Colored marking dyes can be used to help maintain orientation provided they do not interfere with downstream analyses.

[BEFORE_START]
Dilute phosphate buffered saline (Gibco; 14200-075) to 1X with nuclease-free water.

[STEPS]
1. Receive excised female reproductive system on ice en bloc. Orient the specimen with the anterior aspect of the uterus facing up based on presence of vesicouterine reflection.
2. Using a disposable scalpel, incise the mesovarium. Orient ovary with the uteroovarian ligament on the right, infundibulopelvic ligament on the left, and mesovarium on top for the right ovary (Figures 6).

   

3. Dry the ovary thoroughly with a Kimwipe.
4. Use marking dye to maintain orientation of the ovary with the uteroovarian ligament on the right, infundibulopelvic ligament on the left, and mesovarium on top.
5. Take the weight and, using a disposable ruler, take spatial measurement of each ovary.
6. Return ovaries to ice.
7. Place the first ovary in the pre-chilled mold (Figure 2).


  

8. Divide the ovary along the long axis of the ovary from the uteroovarian to the infundibulopelvic ligament using the guidelines of the mold (Figure 1a).
9. Take each slice and orient mesovarium ridge at 12 o’clock.
10. Divide each slice along the line of the mesovarium ridge to divide the ovary into portions superior to and inferior to the mesovarium.
11. Take each half and divide it again in half to separate the ovary into the mesenteric and antimesenteric portions (Figure 1b).
12. For later 3D anatomical positioning, the quadrant of the tissue piece and the slice number along the long axis should be tracked for each tissue specimen.
13. Tissue can be processed with protocols OCT-Embedded Tissue Preparation, Tissue Fixation Preparation, or Snap-Frozen Tissue Preparation depending on the desired downstream processing.
14. Repeat steps with the contralateral ovary, using a fresh, ice-cold ovary mold.
TITLE: DNA extraction (BOMB)
AUTHORS: Yin-Tse Huang, Tsu-Chun Hung

[DESCRIPTION]
DNA extraction (BOMB)

[STEPS]
SECTION: Sample Collection
1. Add 200 µL of 0.5 mm beads to 2mL screw tube
  

SECTION: Sample Collection
2. Add200 µL of 1 mm beads to 2mL screw tube
 
SECTION: Sample Collection
3. Add870 µL Lysis master mix to 2mL screw tube. The final look:
 
 

SECTION: Sample Collection
4. Collect 20-50 mg of sample to 2mL screw tube

 

SECTION: Sample crush
5. Put the 2mL screw tube in mixmill for sample crush, at 3200 rpm  4 min 

 

SECTION: Centrifugation
6. Put 2mL screw tube in centrifuge for centrifugation, at this condition:10 x g, 3 min, 25 °C 
SECTION: DNA purification
7. Add 350 µL of isopropanol to the 1st well of 96 well plate

   

SECTION: DNA purification
0.20. Add 100 µL of magnetic beads (10 mg/ml) to the 1st well of 96 deep well plate 
  
 


SECTION: DNA purification
14. Add 300-500 µL of the sample (lysate) from the 1.5mL centrifuged tube to the 1st well of 96 deep well plate
  
 


SECTION: DNA purification
9. Add 400 µL of isopropanol to the 2nd well of 96 deep well plate
  

SECTION: DNA purification
10. Add 300 µL of 80% ethanol to the 3rd well of 96 deep well plate
 
SECTION: DNA purification
11. Add 300 µL of 80% ethanol to the 4th well of 96 deep well plate
  

SECTION: DNA purification
13. Add 100 µL of DEPC-treated water to the 6th well of 96 deep well plate 
 

SECTION: DNA purification
15. Put the prepared 96 deep well plate in the automated DNA extraction machine and select the BOMB protocol

SECTION: DNA purification
16. After the extraction is done, put on the 96 magnetic plate to pellet the magnetic bead residues.
  

SECTION: DNA purification
12. Add 300 µL of DDW to the 5th well of 96 deep well plate
  

SECTION: DNA purification
17. Collect 100 µL of the eluted sample (avoid getting magnetic bead) as the DNA template for downstream experiments
  

SECTION: DNA purification
8. Add 100 µL of magnetic beads (10mg/ml) to the 1st well of 96 deep well plate
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<div class="page" title="Page 19">
<div class="section">
<div class="layoutArea">
<div class="column">
<p>The ISOLATE II Biofluids RNA Kit isolates total RNA with minimal amounts of genomic DNA contamination using the supplied Genomic DNA Removal Column. However, additional DNase I treatment may be required in certain cases e.g. the amount of genomic DNA in the sample exceeds the capacity of the Genomic DNA Removal Column, or performing a highly sensitive application.</p>
<p> </p>
<p>An alternative additional DNase I treatment protocol is: <a href="https://www.protocols.io/view/appendix-b-optional-dnase-i-treatment-of-purified-f5jbq4n" target="_blank">DNase I Treatment of Purified RNA in Solution</a>.</p>
<p> </p>
<p>This optional protocol can be used for additional removal of residual DNA that may affect sensitive downstream applications.</p>
</div>
</div>
</div>
</div>

[GUIDELINES]
<p>Please review the Guidelines under <a href="https://www.protocols.io/view/genomic-dna-removal-and-total-rna-purification-fro-f48bqzw?guidelines" target="_blank">Genomic DNA removal and total RNA purification from all types of lysate</a> for important details.</p>

[STEPS]
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TITLE: αS PFF Uptake Protocol
AUTHORS: Scott Vermilyea

[DESCRIPTION]
This protocol details the uptake of αS PFF treatement.

[STEPS]
SECTION: αS PFF Uptake Protocol
1. Exchange fresh culture media to cells.

SECTION: αS PFF Uptake Protocol
2. Prepare αS PFF working solution.

Frozen stock is  5 1527, dilute to 0.25 1527 in sterile PBS followed by sonication (1 Sec ON/ 1 Sec OFF) for 2 min with 20% amplitude.

SECTION: αS PFF Uptake Protocol
3. Add αS PFF to cells for a final concentration of 4 1527.

SECTION: αS PFF Uptake Protocol
4. Incubate for desired duration prior to lysate collection.
TITLE: Enzyme-linked immunosorbent assay (ELISA) for detection of anti-HIV antibodies (ELISA) developed in various animal species. 
AUTHORS: Angel  Justiz-Vaillant

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Enzyme-linked immunosorbent assay (ELISA) for studying the presence of anti-HIV antibodies in layer hens, rats and cats was a reproducible and feseable test. The presence of the anti-HIV antibodies was further confirmed by dot blot analysis. </div></div>

[STEPS]
?. The 96 well polystyrene microplates (U-shaped bottom) were coated with 50 ng of the synthetic gp-120 peptide in coating buffer overnight at 4°C.
?. The microplate is washed 4X (PBS-Tween-20) and blocked with 3% non-fat milk in PBS, 25 µl/well, 1h, RT.
?. The microplate is washed 4X (PBS-Tween-20) and triplicates of 25 µl of IgY (1 mg/ml), 25 µl of sera from cats or rats diluted 1:16 in PBS-non fat milk is added.
?. After incubation for 2 h at RT the microplate is washed 4X and 25 µl of a universal conjugate SpLAG-anti-IgY-HRP (comprised of peroxidase labelled-IgY chemically coupled to protein A, protein G and protein L) diluted 1:1000 was added and the plate incubated for a further 1 h at RT.
?. Then, the microplate is washed 4X and after that 3,3’,5,5’-tetramethylbenzidine (Sigma Chemical Co., St Louis, MO, USA) solution (50 µl) is added to each well.
?. After a further incubation of 15 min in the dark, the reaction is stopped when positive controls are coloured and blanks and negative controls do not.
?. Positive controls are from a positive human serum for anti-HIV antibodies, negative controls are from a turtle serum (Sigma Chemical Co., St Louis, MO, USA) and blanks 0.9% normal saline solution.
?. Cut-off point is calculated as the mean of negative controls multiplied by four.
TITLE: PCLS Dehydration
AUTHORS: Morrisey Lab

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">PCLS Dehydration March</div><div class = "text-block">*done at room temperature*</div><div class = "text-block">*make sure to have 2% PFA and etOH pre-aliquoted at room temp*</div></div>

[STEPS]
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Protocol for obtaining high quality RNA from yeast using acid-phenol lysis at 65°C. Modifed from DOI: <a href="https://doi.org/10.1016/S0076-6879(02)50976-9" target="_blank">10.1016/S0076-6879(02)50976-9</a> to use 10-15 ml of yeast culture. Expected yield of 100-200 μg of RNA. For qPCR or RNA-seq, we recommend DNase-treatment and further purification (e.g. Zymo RNA Purification Kit (R1057)).</p>
<p> </p>

[BEFORE_START]
<p>Before starting experiment:</p>
<p>(1) Make or have ready RNase-free stock solutions.</p>
<p>(2) Set up incubating vortex in chemical hood and pre-warm to 65°C.</p>
<p>(3) Pre-label 4 sets of RNase-free 2 ml microcentrifuge tubes for each sample. </p>

[GUIDELINES]
<p>This protocol is written for use with an incubating vortex, such as the MidSci Multi-Therm (MTH-1500). If this is not available, you can incubate samples in a 65°C water bath or heat block and vortex samples every 10-15 min during lysis.</p>

[STEPS]
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TITLE: No Title
AUTHORS: 

[GUIDELINES]
<p><strong>MATERIALS AND ASSAY CONDITIONS:</strong><br /><br /></p>
<p>         1)      Virus infected NC64A <em>chlorella</em> or Pbi <em>chlorella</em>, pellets frozen at -80°C</p>
<p>        <br />         2)      Buffer A:  10 mM Tris-Acetate, pH 8.0, 10 mM 2-ME, 50 µg/mL PMSF</p>
<p>         <br />         3)      Buffer B:  20 mM Tris-Acetate, pH 8.0, 0.5 mM EDTA, 7 mM 2-ME, 10% Glycerol<br /><br /></p>
<p>         4)      Buffer B, pH 8.5:  20 mM Tris-Acetate, pH 8.5, 0.5 mM EDTA, 7 mM 2-ME<br /><br /><br />         5)      Storage buffer: 20 mM Tris-Acetate, pH 8.0, 0.5 mM EDTA, 0.1 mM DTT, 50 mM KOAc, 5 mM MgAc, 50% Glycerol, 100 µg/mL BSA (the BSA is added after dialysis into the storage buffer from a 10 mg/mL stock)<br /><br /></p>
<p>          6)      4 M NaCl<br /><br /></p>
<p>          7)      28% Polyethyleneglycol (PEG) 8000</p>
<p> </p>
<p>         8)      Assay buffer and conditions:</p>
<p>                           1X CviJI assay buffer:</p>
<p>                                    20 mM GlycylGlycine, pH 8.5 (with KOH)</p>
<p>                                    10 mM MgAc</p>
<p>                                    0.1 mM DTT</p>
<p>                                    50 mM KOAc</p>
<p>                                    100 ug/ml casein (optional)</p>
<p> <br />All assays are carried out in 20.0 ul volumes with 1 ug of pUC19 DNA as substrate for 60 to 120 min at 25°C.  The assays are electrophoresed on 2.0% agarose gels (100 ml gels with double 20 lane combs) for 1 hour at 100 volts in 1X TPE buffer.  Gels are stained with 0.5 µg/ml ethidium bromide for 30 min and photographed on a UV light box.</p>

[STEPS]
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TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Here we present a neural differentiation protocol for generating neural progenitor cells (NPCs) from human induced pluripotent stem cells (hiPSCs). Briefly, human dermal fibroblasts were reprogrammed into hiPSCs via non-integrative reprogramming approach. Subsequently, hiPSCs were differentiated into NPCs by forming neural rosettes <em>in vitro</em>. Characteristic morphological changes were observed and neural markers were highly enriched in the rosettes stage.</p>

[STEPS]
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TITLE: Western Blotting (Fly Heads)
AUTHORS: Mel Feany

[DESCRIPTION]
This protocol describes how to perform a Western Blotting technique using fly heads.

[STEPS]
1. Homogenize desired number of fly heads in 1 X Laemmli sample buffer.
2. Heat samples to 100 °C for 10 min , spin briefly before loading.

3. Load premade gel into western blotting apparatus. Fill reservoir with Running Buffer:
Running Buffer:
6 g  Tris-HCL
28.9 g  glycine
Fill to 1 L  with distilled water
Add 5 mL  20% SDS

4. Load samples on gel and attach electrodes.
5. Run gel at 120 V until dye front reaches the bottom of the gel, 60 min. Run longer for greater separation.

6. Remove gel and transfer using Trans-Blot Turbo.
7. Perform antigen retrieval by microwaving 9 min  in PBS.

8. Block membrane in 1X PBS with 0.05% Tween-20 and 3% dry milk for 60 min.

9. Add primary antibody at correct dilution in PBSTween + milk and incubate with shaking    at 4 °C.

10. Wash blot 3x in PBSTween, 5 min each, with shaking.

11. Add secondary antibody at the correct dilution in PBSTween + milk, incubate with shaking at Room temperature  for 180 min.

12. Wash blot in PBSTween 30 min  with frequent wash changes.

13. Develop with ECL substrate or image fluorescence, as appropriate.
TITLE: Spatial Transcriptomics for FFPE utilizing 10x Genomics Visium 
AUTHORS: Andrew Houston, Siqi Chen, Feng Chen

[DESCRIPTION]
To detect gene expression spatially mapped onto formalin-fixed paraffin-embedded tissue samples adapted from the 10x Genomics Visium FFPE v1 protocol. 

[STEPS]
SECTION: Quality Assessment
1. Follow the tissue preparation guide as outlined below to determine if your starting FFPE tissue block is acceptable for the 10x Visium platform
Start here
Utilize Visium Tissue Section Test Slide (PN-2000460) to do a quality assessment of each sample
Be sure to trim a few sections and place in a 1.5ml tube for RNA quality check (no more than 40μm)
Follow the steps starting on pg. 10 of the above user guide
SECTION: Quality Assessment
3. Perform RNA extraction using the Qiagen RNeasy FFPE kit (Cat no. 73504)
Follow the steps outlined in the RNeasy Handbook which can be found below
  
Start with your pre-cut sections in a 1.5ml tube on pg. 15

SECTION: Quality Assessment
4. After RNA extraction test the concentration of your samples using a nanodrop
Determine the DV200 value of your samples utilizing the Agilent 4200 TapeStation system or similar quantitative assessment
If utilizing Agilent TapeStation to prepare your RNA samples utilize this guide
For first time analysis of DV200 you can set up your parameters as described here
SECTION: Visium FFPE v1
5. If the tissue adheres to the slide and has a DV200 value >50% that sample can be used for the Visium Spatial Transcriptomics platform
SECTION: Visium FFPE v1
6. Utilize the CG000408 linked above to section onto the Visium Spatial Gene Expression Slide (PN-2000233)
SECTION: Visium FFPE v1
7. After sectioning start the protocol utilizing CG000409 linked above to perform deparaffinization/staining/imaging steps
Be sure to either store your slide or perform decrosslinking to proceed with the rest of the protocol outlined in the next step

SECTION: Quality Assessment
2. Perform all deparaffinization/staining/imaging steps utilizing the test slide
Follow the protocol as described by 10x here
SECTION: Visium FFPE v1
8. If decrosslinking is complete, utilize the following user guide outlined here
Start probe hybridization on pg. 39

SECTION: Visium FFPE v1
9. Follow the sequencing requirements on pg. 60 to generate your Visium libraries
TITLE: Diaetoxil 600mg Avis- Medical, Prix Pharmacie, Arnaque Forum
AUTHORS: Diaetoxil  France

[DESCRIPTION]
  

Diaetoxil est formulé pour déclencher une perte de poids naturelle, ce qui permet aux individus d'avoir un corps maigre et un indice de masse corporelle (IMC) sain. En plus d'augmenter le métabolisme des graisses, les fabricants de Diaetoxil affirment également que le supplément de perte de poids induit la satiété pour le contrôle des calories. Par conséquent, il contient quatre ingrédients naturels pour faciliter le processus naturel de perte de poids. Prenez le temps de passer en revue tous les facteurs et découvrez à quel type de résultats vous pouvez vous attendre lorsque vous commencez à prendre le supplément afin que vous sachiez que vous avez fait le bon choix. Explorons les résultats à attendre de ce supplément. Vous devez vous rappeler ici que ce que nous avons présenté ci-dessous n'est qu'une représentation indicative des résultats. Le taux de réponse variera d'une personne à l'autre.  Le BHB endogène est un composé chimique que le corps produit lorsqu'il est affamé de glucides ou d'hypoglycémie pour faciliter la production d'énergie. Cependant, comme expliqué ci-dessus, le corps peut parfois ne pas produire suffisamment de BHB pour faciliter le métabolisme durable des graisses. La cétone BHB (bêta-hydroxybutyrate) est le principal ingrédient actif de la formule de réduction des graisses de Diaetoxil. De telles incidences ont inspiré la création de BHB exogène administré à des personnes manquant de suffisamment de BHB endogène pour déclencher la cétose. Outre l' élévation du taux métabolique pendant l'exercice physique, la cétone de framboise est supposée fonctionner en augmentant les taux métaboliques de base et au repos. Le taux métabolique de base fait référence à la quantité d'énergie que votre corps expulse pour maintenir les fonctions vitales telles que la respiration. 


Clic Web officiel :-

https://ipsnews.net/business/2022/06/29/diaetoxil-gelules-avis-france-2022-pharmercie-prix-perte-de-poids-capsules-arnaque-or-acheter/


https://techplanet.today/post/diaetoxil-600mg-avis-medical-prix-pharmacie-arnaque-forum

https://www.qnapclub.fr/topic/15606-diaetoxil-600mg-avis-medical-prix-pharmacie-arnaque-forum/

https://diaetoxil-france-avis.clubeo.com/page/diaetoxil-600mg-avis-medical-prix-pharmacie-arnaque-forum.html

https://zenodo.org/record/6826685#.Ys6lM3ZBzIU

https://www.homify.fr/projets/1161950/diaetoxil-600mg-avis-medical-prix-pharmacie-arnaque-forum

https://techplanet.today/post/diaetoxil-600mg-avis-medical-prix-pharmacie-arnaque-forum

https://keithburgun.net/forum/index.php?threads/diaetoxil-600mg-avis-medical-prix-pharmacie-arnaque-forum.466/

https://www.qnapclub.fr/topic/15606-diaetoxil-600mg-avis-medical-prix-pharmacie-arnaque-forum/

https://connect.scopito.com/post/diaetoxil-600mg-avis--medical-prix-pharmacie-arnaque-forum-62cea7804b8e8176e4c12481

https://diaetoxilfra.cgsociety.org/eg5p/diaetoxil-600mg-avis


https://zenodo.org/record/6826685#.Ys6lM3ZBzIU




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