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[DESCRIPTION]
<p>This protocol is for Tube-O-DIALYZER™, a mini dialysis system for small sample volumes (20µl‐2.5ml). Follow this protocol for dialysis devices Cat. # <a href="http://www.gbiosciences.com/ResearchProducts/tubeodialyzer-desc.aspx" target="_blank">786‐610 to 786‐624</a>.</p>

[GUIDELINES]
<p><strong>INTRODUCTION</strong></p>
<p>Tube-O-DIALYZER<sup>TM</sup> is a mini dialysis system for small sample volumes (20µl-2.5ml) that ensures 100% sample recovery, even if precipitation occurs. Tube-O-DIALYZER<sup>TM</sup> is based on a patented tube design that permits easy sample handling and a dialysis cap. Simply pipette your sample into theTube-O-DIALYZER<sup>TM</sup> tube, seal with theTube-O-DIALYZER<sup>TM</sup> dialysis cap and invert in your dialysis solution and dialyze. Following dialysis, briefly centrifuge the complete Tube-O-DIALYZER<sup>TM</sup> for five seconds and collect 100% of your sample. For storage of the dialyzed sample, replace the dialysis cap with the supplied storage caps.</p>
<p> </p>
<p>Tube-O-DIALYZER<sup>TM</sup> consists of a regenerated cellulose dialysis membrane, a neutral non binding dialysis membrane, with Molecular Weight Cut Offs (MWCO) of 1,000, 4,000, 8,000, 15,000, or 50,000 Daltons.</p>
<p><img id="s-mce-img" class="s-mce-img" src="https://s3.amazonaws.com/pr-journal/dwscz36.png" data-src="https://s3.amazonaws.com/pr-journal/dwrcz36.png" data-ofn="Screen Shot 2016-06-08 at 4.00.24 PM.png" /></p>
<p><strong>ITEM(S) SUPPLIED</strong></p>
<table style="height: 48px; width: 462px;">
<tbody>
<tr>
<td style="width: 134px;"> </td>
<td style="width: 336px;"><strong>Tube‐O‐DIALYZER™, Micro For 20‐250µl Samples</strong></td>
</tr>
</tbody>
</table>
<table style="width: 467px; height: 259px;">
<tbody>
<tr style="height: 40px;">
<td style="width: 147px; height: 40px;">Cat. #</td>
<td style="width: 59px; height: 40px;"><strong>786‐610</strong></td>
<td style="width: 68px; height: 40px;"><strong>786‐611</strong></td>
<td style="width: 67px; height: 40px;"><strong>786‐612</strong></td>
<td style="width: 79px; height: 40px;"><strong>786‐613</strong></td>
<td style="width: 71px; height: 40px;"><strong>786‐614</strong></td>
</tr>
<tr style="height: 20px;">
<td style="width: 147px; height: 20px;">MWCO (Da)</td>
<td style="width: 59px; height: 20px;">1,000</td>
<td style="width: 68px; height: 20px;">4,000</td>
<td style="width: 67px; height: 20px;">8,000</td>
<td style="width: 79px; height: 20px;">15,000</td>
<td style="width: 71px; height: 20px;">50,000</td>
</tr>
<tr style="height: 60px;">
<td style="width: 147px; height: 60px;">Tube‐O‐DIALYZER™ , Micro</td>
<td style="width: 59px; height: 60px;">20</td>
<td style="width: 68px; height: 60px;">20</td>
<td style="width: 67px; height: 60px;">20</td>
<td style="width: 79px; height: 60px;">20</td>
<td style="width: 71px; height: 60px;">20</td>
</tr>
<tr style="height: 40px;">
<td style="width: 147px; height: 40px;">Floats (Mirco)</td>
<td style="width: 59px; height: 40px;">6</td>
<td style="width: 68px; height: 40px;">6</td>
<td style="width: 67px; height: 40px;">6</td>
<td style="width: 79px; height: 40px;">6</td>
<td style="width: 71px; height: 40px;">6</td>
</tr>
<tr style="height: 60px;">
<td style="width: 147px; height: 60px;">Stronage Caps (Mirco)</td>
<td style="width: 59px; height: 60px;">20</td>
<td style="width: 68px; height: 60px;">20</td>
<td style="width: 67px; height: 60px;">20</td>
<td style="width: 79px; height: 60px;">20</td>
<td style="width: 71px; height: 60px;">20 </td>
</tr>
</tbody>
</table>
<p> </p>
<p> </p>
<table style="width: 470px;">
<tbody>
<tr>
<td style="width: 102px;"> </td>
<td style="width: 370px;"><strong>Tube‐O‐DIALYZER™ , Medi For 0.2‐2.5ml Samples</strong></td>
</tr>
</tbody>
</table>
<table style="width: 472px;">
<tbody>
<tr>
<td style="width: 56px;">Cat. #</td>
<td style="width: 39px;"><strong>786‐615</strong></td>
<td style="width: 42px;"><strong>786‐616</strong></td>
<td style="width: 52px;"><strong>786‐617</strong></td>
<td style="width: 48px;"><strong>786‐618</strong></td>
<td style="width: 40px;"><strong>786‐619</strong></td>
</tr>
<tr>
<td style="width: 56px;">MWCO (Da)</td>
<td style="width: 39px;">1,000</td>
<td style="width: 42px;">4,000</td>
<td style="width: 52px;">8,000</td>
<td style="width: 48px;">15,000</td>
<td style="width: 40px;">50,000</td>
</tr>
<tr>
<td style="width: 56px;">Tube‐O‐DIALYZER™ , Medi</td>
<td style="width: 39px;">20</td>
<td style="width: 42px;">20</td>
<td style="width: 52px;">20</td>
<td style="width: 48px;">20</td>
<td style="width: 40px;">20</td>
</tr>
<tr>
<td style="width: 56px;">Floats (Medi)</td>
<td style="width: 39px;">6</td>
<td style="width: 42px;">6</td>
<td style="width: 52px;">6</td>
<td style="width: 48px;">6</td>
<td style="width: 40px;">6</td>
</tr>
<tr>
<td style="width: 56px;">Storage Caps (Medi)</td>
<td style="width: 39px;">
<p>20</p>
</td>
<td style="width: 42px;">20</td>
<td style="width: 52px;">20</td>
<td style="width: 48px;">20</td>
<td style="width: 40px;">20 </td>
</tr>
</tbody>
</table>
<p> </p>
<p> </p>
<table style="width: 473px;">
<tbody>
<tr>
<td style="width: 151px;"> </td>
<td style="width: 326px;"><strong>Tube‐O‐DIALYZER™ , Mixed For 20‐250µl &amp; 0.2‐2.5ml Samples</strong></td>
</tr>
</tbody>
</table>
<table style="width: 475px;">
<tbody>
<tr>
<td style="width: 149px;">Cat. #</td>
<td style="width: 54px;"><strong>786‐620</strong></td>
<td style="width: 55px;"><strong>786‐621</strong></td>
<td style="width: 55px;"><strong>786‐622</strong></td>
<td style="width: 62px;"><strong>786‐623</strong></td>
<td style="width: 72px;"><strong>786‐624</strong></td>
</tr>
<tr>
<td style="width: 149px;">MWCO (Da)</td>
<td style="width: 54px;">1,000</td>
<td style="width: 55px;">4,000</td>
<td style="width: 55px;">8,000</td>
<td style="width: 62px;">15,000</td>
<td style="width: 72px;">50,000</td>
</tr>
<tr>
<td style="width: 149px;">Tube‐O‐DIALYZER™ , Micro</td>
<td style="width: 54px;">10</td>
<td style="width: 55px;">10</td>
<td style="width: 55px;">10</td>
<td style="width: 62px;">10</td>
<td style="width: 72px;">10</td>
</tr>
<tr>
<td style="width: 149px;">Tube‐O‐DIALYZER™ , Medi</td>
<td style="width: 54px;">10</td>
<td style="width: 55px;">10</td>
<td style="width: 55px;">10</td>
<td style="width: 62px;">10</td>
<td style="width: 72px;">10</td>
</tr>
<tr>
<td style="width: 149px;">Floats (Micro)</td>
<td style="width: 54px;">3</td>
<td style="width: 55px;">3</td>
<td style="width: 55px;">3</td>
<td style="width: 62px;">3</td>
<td style="width: 72px;">3</td>
</tr>
<tr>
<td style="width: 149px;">Floats (Medi)</td>
<td style="width: 54px;">3</td>
<td style="width: 55px;">3</td>
<td style="width: 55px;">3</td>
<td style="width: 62px;">3</td>
<td style="width: 72px;">3</td>
</tr>
<tr>
<td style="width: 149px;">Storage Caps (Micro)</td>
<td style="width: 54px;">10</td>
<td style="width: 55px;">10</td>
<td style="width: 55px;">10</td>
<td style="width: 62px;">10</td>
<td style="width: 72px;">10</td>
</tr>
<tr>
<td style="width: 149px;">Storage Caps (Medi)</td>
<td style="width: 54px;">10</td>
<td style="width: 55px;">10</td>
<td style="width: 55px;">10</td>
<td style="width: 62px;">10</td>
<td style="width: 72px;">10 </td>
</tr>
</tbody>
</table>
<p> </p>
<p> </p>
<p><strong>STORAGE CONDITIONS </strong></p>
<p>The kit is shipped at ambient temperature. Upon arrival, store at 4°C. Stable for 1 year, if stored correctly.  </p>
<p> </p>
<p> </p>
<p>Please see <a href="http://www.gbiosciences.com/PDF/Protocol/Tube-O-Dialyzer.pdf" target="_blank">G-Biosciences Tube‐O‐DIALYZER™</a> for details on Membrane Compatibility.</p>
<p> </p>
<p><strong>OPTIONAL ACCESSORIES</strong></p>
<p><strong>• Centrifuge Tube Adaptor [Cat # 786‐145]:</strong> Optional adaptor for centrifugation in bench top centrifuges. Pack of 2.</p>
<p><strong>• Micro Dialysis Cups [Cat # 786‐145C]:</strong> For dialysis of small sample volumes, equilibrium dialysis, dialysis of single use preparations, and other dialysis applications. Dialysis buffer capacity of 2‐10ml.</p>
<p><strong>• Tube‐O‐Tanks [Cat # 786‐145D &amp; 786‐145E]:</strong> A dialysis tank specifically designed for use with Tube‐O‐DIALYZER™ . Two sizes are available, small and large tanks, suitable for Micro and Medi size Tube‐O‐DIALYZER™ , respectively. Each tank will accommodate seven Tube‐O‐DIALYZER™ .</p>
<p><strong>• Tube‐O‐Array™ Dialyzer [Cat # 786‐145A]:</strong> Specifically developed for dialysis‐ equilibration of samples prior to 2D‐gel analysis or other applications. Optimize up to 12 samples at a time. Consists of a tray for holding up to 12 Tube‐O‐DIALYZER™ assemblies. Suitable for 20μl to 2.5ml samples each.</p>
<p><strong>• Floats:</strong>  Additional Tube‐O‐DIALYZER floats, both Micro [Cat # 786‐141F] and Medi [Cat # 786‐142F] sizes are also available separately</p>
<p> </p>
<p><strong>TROUBLESHOOTING </strong></p>
<p>1.<strong> Reusing Tube‐O‐DIALYZER™ :</strong> Tube‐O‐DIALYZER™ is not recommended for re‐use because of obvious reason of cross contamination.</p>
<p>2.<strong> Precipitate Forms in Sample:</strong> As with all dialysis methods, precipitation may occur in your sample.  The unique tube format means the entire Tube‐O‐DIALYZER™ can be centrifuged to collect the precipitate.  We recommend centrifuging for 5‐6 seconds at 500‐1,000xg. Check the sample and if necessary repeat the centrifugation.  Do not spin longer as this may cause the membrane to rupture.</p>
<p>3. <strong>Mishandling Dislodges White Gasket:</strong> In some cases the white gasket inside the Tube‐O‐DIALYZER™ cap can be dislodged by mishandling. In this event, we recommend using the base of the Tube‐O‐DIALYZER™ tube to push the gasket back in to position.</p>

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