eamag/protocols-with-steps huggingface dataset:
TITLE: αS PFF Assembly Protocol
AUTHORS: Scott Vermilyea

[DESCRIPTION]
This protocol details the αS PFF assembly.

[STEPS]
SECTION: Aggregation
1. Remove monomer stock from -80 °C and thaw on ice.
SECTION: Aggregation
2. Dilute stock to desired concentration (typically 5 3189) to a final volume of 300 µL in autoclaved2 mLcentrifuge tubes. 
SECTION: Aggregation
3. Set thermocycler for continuous shaking (1000 rpm, 37 °C ).
SECTION: Aggregation
4. Install 2 mL plate.
SECTION: Aggregation
5. Run PBS and Protein samples. Take 2 µL aliquot at 0 hr time point. Run ThioT Assay.
SECTION: Aggregation
6. Take 2 µL aliquots twice per day for up to 7200 min ( 72 hours - 96 hours may be sufficient).
SECTION: Aggregation
7. Check ThioT Fluorescence.
SECTION: Aggregation
8. Aliquot and store at -80 °C.
SECTION: ThioT Assay
9. Prepare ThioT stock mixture (8 µg ThioT in 10 µL milliQ H2O).
SECTION: ThioT Assay
10. Prepare working ThioT mixture, 20 µL stock into 1 mL PBS.
SECTION: ThioT Assay
11. Dispense 200 µL working solution into each 2 µL aliquot.
SECTION: ThioT Assay
12. Dispense 200 µL per sample into 96-well, black, corning glass-bottomed plate.
SECTION: ThioT Assay
13. Read Fluorescence Intensity (Ex 450 +/-10; Em 490 +/- 10).
TITLE: DNA extraction (BOMB)
AUTHORS: Tsu-Chun  Hung, Yin-Tse    Huang

[DESCRIPTION]
DNA extraction (BOMB)

[STEPS]
SECTION: Sample Collection
1. Add 200 µL of 1mm beads to 1.5ml enppendorf tube

SECTION: Sample Collection
2. Add200 µL of 0.5mm beads to 1.5ml enppendorf tube

SECTION: Sample Collection
3. Add 225 µL of TE buffer to 2ml enppendorf tube
 

SECTION: Sample Collection
4. Add 375 µL of lysis buffer to 2ml enppendorf tube
 


SECTION: Sample Collection
6. Collect 10-20 mg of sample to 2ml enppendorf tube
SECTION: Sample crush
7. Put 2ml eppendorf tube in mixmill for sample crush, at this condition: 30 rpm/s, for 4mins 4 min 
SECTION: Centrifugation
8. Put 2ml eppendorf tube in centrifuge for centrifugation, at this condition:17.0 x g, 3 min, 25 °C 
SECTION: DNA purification
9. Add 350 µL of isopropanol to the 1st well of 96 well plate

   

SECTION: DNA purification
9.1. Add 125 µL of magnetic beads (10 mg/ml) to the 1st well of 96 deep well plate 

 


SECTION: DNA purification
9.2. Add 200 µL of the sample (lysate) from the 2ml centrifuged tube to the 1st well of 96 deep well plate 
 

SECTION: DNA purification
10. Add 400 µL of isopropanol to the 2nd well of 96 deep well plate 
SECTION: DNA purification
11. Add 300 µL of 80% enthanol to the  3rd well of 96 deep well plate 
SECTION: DNA purification
12. Add 300 µL of 80% enthanol to the 4th well of 96 deep well plate 
SECTION: DNA purification
13. Add 100 µL of DD water to the 5th well of 96 deep well plate 
SECTION: DNA purification
14. Put the prepared 96 deep well plate in the automated DNA extraction machine

SECTION: Sample Collection
5. Add 267 µL of 10M ammonium acetate to 2ml enppendorf tube
 

SECTION: DNA purification
15. After the extraction is done, collect 100 µL of the eluted sample as the DNA template for downstream experiments
TITLE: Co-immunoprecipitation
AUTHORS: wusj, schekman

[DESCRIPTION]
This protocol describes a common procedure to perform co-immunoprecipitation

[STEPS]
SECTION: Co-immunoprecipitation
1. Media fractions were collected and centrifuged at 1000×g for 10 min

SECTION: Co-immunoprecipitation
2. The supernatant fractions were collected and concentrated (20-fold) using a 10 kDa Amicon filter. 
SECTION: Co-immunoprecipitation
3. Concentrated media fractions (1 ml) were incubated with 20 μl of anti-FLAG M2 affinity gel for 60 min at 4 °C

SECTION: Co-immunoprecipitation
4. After washing 5 times with lysis buffer , SDS-PAGE sample loading buffer was added to the beads (twice the beads volume) and samples were processed for SDS-PAGE and immunoblot.
TITLE: Protocol: Evaluation of the quality of reporting and risk of bias of predictive models widely used in UK perioperative practice
AUTHORS: Joseph Alderman

[DESCRIPTION]
The aim of this targeted literature review is to critically appraise the reporting and methodological robustness of studies describing the development of predictive models which are most widely used in UK perioperative practice. This is not intended to be a systematic review of the evidence supporting each model - more a review of the state of evidence in general to inform the design of future research studies and develop policy recommendations.

Objectives include:
Apply the TRIPOD+AI and PROBAST frameworks to papers describing development of predictive models used frequently in perioperative practice.
Summarise the clinical context and population groups represented in any development and validation datasets.
Identify the ‘intended use’ of PMCS being investigated.
Identify actionable recommendations for future development/validation studies by comparing limitations across literature sources.

[STEPS]
TITLE: No Title
AUTHORS: 

[GUIDELINES]
<table>
<tbody>
<tr>
<td>Tryptone</td>
<td>5 g</td>
</tr>
<tr>
<td>Yeast Extract</td>
<td>1 g</td>
</tr>
<tr>
<td>Seawater</td>
<td>1 L</td>
</tr>
<tr>
<td>Dry Ice</td>
<td>10-12 g</td>
</tr>
</tbody>
</table>
For bottom agar plates, add 15 g agar.<br />For top agar, add 4 g agar (or 4 g LMP agar)<br /><br />-Heat to dissolve agar.<br />-Autoclave.<br />-Cool in water bath to 41&deg;C for top agar or to 50&deg;C to pour bottom agar plates.<br /><br />*Rohwer et al (2000) Liminol. Oceanogr. 45: 408-418 used 0.01g FeC6H5O7 (iron citrate) per liter of 80% 0.45 &micro;m filtered seawater and called it ZoBell 2216E medium.

[STEPS]
TITLE: Electroporation of Heterosigma akashiwo
AUTHORS: Deepak Nanjappa and Kathryn Coyne

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>This protocol was developed for transformation of </span><span style = "font-style:italic;">Heterosigma akashiwo </span><span>by electroporation. This species does not grow on solid medium, so transformants are grown "in bulk" and should not be considered clonal. We have had limited success with this protocol, which may need to be further optimized.</span></div></div>

[STEPS]
?. Harvest exponential growth Heterosigma akashiwo culture (1-2 x 106 cells mL-1) by centrifugation at 1500 rpm for 90 s in a 50 mL sterile screw-cap tube. Remove the supernatant by pipetting.
?. Resuspend the cell pellet in a small volume of MAX Efficiency® Transformation Reagent for Algae (Invitrogen, Thermo Fischer Scientific, USA) or in 384mM Sorbitol.
?. Transfer 200µl of cells into a 1.5ml Eppendorf tube and add upt to 20 µl (at least 1µg) of linearized plasmid DNA. Mix by tapping the mixture and incubate at room temperature for 10 mins. See DOI: 10.5281/zenodo.439653 for plamid sequence information.
?. Transfer the mixture to a 4 mm gap cold electroporation cuvette (BioRad). Electroporate in an electroporator, BioRad® Gene Pulser® (BioRad) at 50/75 Volts, 25 µF and ∞Ω and let it sit on bench at room temperature for 15mins. Typical electro pulse duration obtained is about 10–15 ms.
?. Transfer the electroporated sample in equal amounts to 4 wells of 12 well plate with 2 ml of fresh f/2 medium with cefotaxime (100µg/ml, to control bacterial growth).
?. Incubate in dim light for 1 day and then transfer to normal light conditions for another 2 days. After 3–4 days, add appropriate antibiotic and incubate in normal conditions.
?. [Expected Results]
Transformants should be visible after 20-30 days. Continue to transfer the live cells to fresh medium every 10-12 days with antibiotic and analyse transformants by PCR once sufficient culture is obtained.
TITLE: Preparation of 0.5L of phosphate buffer (pH=6.0)
AUTHORS: Alfonso  Pérez Escudero, Alid Al-Asmar, gabrielmadirolas

[DESCRIPTION]
Original source of the protocol: WormBook Methods http://www.wormbook.org/chapters/www_strainmaintain/strainmaintain.html
This protocol is for making pH=6.0 phosphate buffer, which is used in the preparation of worm media, like NGM (Nematode Growth Medium). 

[STEPS]
1. Add 54.2 g of monopotassium phosphate1 (KH2PO4) to a clean 0.5L bottle.

1Also referred to as potassium phosphate monobasic
2. Add 17.8 g of dipotassium phosphate2 (K2HPO4) to the bottle.

2Also referred to as potassium phosphate dibasic
3. Measure 500 mL of milliQ water in a measuring cylinder, and add it to the bottle.

4. Shake the bottle vigorously until all crystals are dissolved. This can take a few minutes.
5. If this is the first time you prepare this solution, you can ensure that the pH is around 6 using pH paper or a pH-meter. We usually have a pH between 5.8 and 6. 
7. Store on shelf.
We usually use it without problem for at least a year after preparation.
6. Filter-sterilize.
TITLE: House Fly (Musca domestica) laboratory rearing protocol - Gerry lab (UC Riverside)
AUTHORS: Levi  Zahn, Alec  Gerry

[DESCRIPTION]
This protocol is designed for large scale laboratory rearing of house flies at very low cost.

[BEFORE_START]
If you are collecting flies from a field site to begin your house fly colony, be sure to keep field-collected adult flies away from any existing fly colonies or colony supplies.  Field flies are likely to carry several fungal and bacterial pathogens that can infect your existing fly colonies.  Instead, hold field flies separately and collect eggs from these flies that can then be placed into your colony rearing rooms without infecting other colonies.

[GUIDELINES]
This protocol is designed for large scale laboratory rearing of house flies at very low cost.

[STEPS]
TITLE: Ann Marie&#39;s Sugar Cookies
AUTHORS: Hannah Gunderman

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Check out this recipe from Ann Marie Mesco, our Digitization Projects Manager and Data Curator for KiltHub!</div><div class = "text-block">Photo by Cayla1 on Unsplash. </div></div>

[STEPS]
?. [Overview]
This is a holiday favorite to cut into shapes for Christmas, Easter, Halloween and other special occasions. Adorn them with colored sugar sprinkles, dried fruit bits or tinted frosting. Why not try some pink frosting for Valentine's Day?Makes: about 4 dozen
?. [Overview]
Ingredients1/2 cup butter, room temperature  1/2 cup margarine, room temperature  1 1/4 cups sugar   2 eggs                     1 tsp. vanilla extract  1/4 tsp. almond extract   3 1/2 cups all-purpose flour  1 tsp. baking powder  1/4 tsp. salt
?. [Overview]
Beat butter and margarine until creamy. Beat in sugar, eggs, vanilla and almond extract. In a separate bowl, stir together flour, baking powder and salt. Add to creamed mixture and beat well.
?. [Overview]
Bake in a 350° oven for 8 to 10 minutes, or until lightly browned. Remove immediately to wire racks and cool.
?. [Overview]
Share with a special someone! This includes sharing them with yourself, because you are special!
?. [Overview]
Cover and chill at least 1 hour. Roll dough to l/8-inch thickness on a lightly floured board. Cut into desired shapes. Place on greased baking sheets and sprinkle with colored sugar if desired.
TITLE: Magnetic bead cleaning of PCR products
AUTHORS: Alexander Eiler

[DESCRIPTION]
<div class = "text-blocks"></div>

[STEPS]
?. Calculate the volume needed for the beads:0.8 * sample volume =
?. Shake the magnetic bead buffer bottle to fully resuspend magnetic particles.
?. Add Sample.
?. Add beads.
?. Pipette mix 10 times and Incubate at room temperature for 5 minutes.
?. Place the reaction plate onto a Magnet Plate for 2 minutes to separate beads from solution. (Keep on the magnet plate until step 11).
?. Aspirate the supernatant from the reaction plate and discard.
?. Add 40 μL of elution buffer, pipette mix 10 times. Make sure the buffer reaches up to the beads!
40 µl
?. Place the reaction plate onto a Magnet Plate for 1 minute to separate beads from solution.
?. Transfer purified product to a new PCR plate or strips.
?. Dispense 200 μL of 70% ethanol and incubate at room temperature for at least 30 seconds. Aspirate out the ethanol and discard. You can discard the ethanol by inverting the plate keeping the magnetic plate on the bottom of it.
200 µl
?. Dry off the remaining ethanol. To speed up the process, you can place the plate on a warm (30°C) heat block.Drying takes at least 30 minutes. Check that drying was complete before continuing.
?. Repeat the washing step as above: dispense 200 μL of 70% ethanol and incubate at room temperature for at least 30 seconds. Aspirate out the ethanol and discard. You can discard the ethanol by inverting the plate keeping the magnetic plate on the bottom of it.
200 µl
?. Remove the remaining ethanol by pipetting.
?. Take the plate off the magnet
?. Incubate at room temperature for 2 minutes.
TITLE: Slide Preparation for Mounting Samples for TissueCyte 1000 Sectioning and Imaging
AUTHORS: Allen Institute for Brain Science

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes the preparation of 1x3 glass slides for mounting embedded mouse brain samples for subsequent imaging and sectioning on TissueCyte 1000. </div><div class = "text-block"><span style = "font-weight:bold;">Note</span><span>: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.</span></div></div>

[STEPS]
TITLE: No Title
AUTHORS: 

[BEFORE_START]
<span style="text-decoration: underline;"><strong>Input Files</strong></span><br /><br />You must have the appropriate input files in the displayed directory structure:<br /><br /><em>Multi-parametric mapping (MPM) input files:</em><br />
<ul>
<li><strong>R1.nii.gz</strong> -- 3D NIFTI image containing longitudinal relaxation rate (R1) values in MPM subject space</li>
<li><strong>R2s.nii.gz</strong> -- 3D NIFTI image containing transverse relaxation rate (R2s) values in MPM subject space</li>
<li><strong>MT.nii.gz</strong> -- 3D NIFTI image containing magnetisation transfer (MT) values in MPM subject space</li>
<li><strong>A.nii.gz</strong> -- 3D NIFTI image containing proton density (A) values in MPM subject space</li>
<li><strong>PDw.nii.gz</strong> -- 3D NIFTI image containing proton density weighted (PDw) values in MPM subject space</li>
</ul>
<strong><br /></strong><em>Diffusion weighted imaging (DWI) imaging input files:</em><br />
<ul>
<li><strong>dti.nii.gz</strong> -- 4D NIFTI image containing 63 diffusion weighted acquisition and 6 non-weighted acquistions (total N=69) in DTI subject space</li>
<li><strong>bvals</strong> -- text file containing one row of 69 values representing the diffusion weightings for the 69 volumes in <strong>dti.nii.gz</strong></li>
<li><strong>bvecs</strong> -- text file containing three rows of 69 values representing the x, y, and z components of the diffusion directions for the 69 volumes in <strong>dti.nii.gz</strong></li>
</ul>
<br /><br /><span style="text-decoration: underline;"><strong>Software Installations</strong></span><br /><br />You must also have the following software libraries installed:<br />
<ul>
<li><strong>Freesurfer</strong>: <a href="https://surfer.nmr.mgh.harvard.edu/fswiki/DownloadAndInstall" target="_blank">https://surfer.nmr.mgh.harvard.edu/fswiki/DownloadAndInstall</a></li>
<li><strong>FSL</strong>: <a href="http://fsl.fmrib.ox.ac.uk/fsl/fslwiki/FslInstallation" target="_blank">http://fsl.fmrib.ox.ac.uk/fsl/fslwiki/FslInstallation</a></li>
<li><strong>Anaconda</strong>: <a href="https://www.continuum.io/downloads" target="_blank">https://www.continuum.io/downloads</a></li>
</ul>
<br />From within <strong>Anaconda</strong> install the following python packages:<br />
<ul>
<li><a href="http://www.vtk.org/VTK/resources/software.html#latestcand" target="_blank">VTK</a> by typing <code>conda install vtk</code></li>
<li><a href="http://mayavi.sourceforge.net/install.html" target="_blank">Mayavi</a> by typing <code>conda install mayavi</code></li>
<li><a href="http://nipy.sourceforge.net/nibabel/installation.html#installation" target="_blank">Nibabel</a> by typing <code>pip install nibabel</code></li>
<li><a href="http://pysurfer.github.io/install.html" target="_blank">pysurfer</a> by typing <code>pip install pysurfer</code></li>
</ul>

[STEPS]
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TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>The NA inhibition test using large molecular fetuin substrate is believed to be the most specific method to detect antibodies against influenza virus neuraminidase. To avoid cross-reacting with hemagglutination-inhibition antibodies we have prepared several reassortant A/H7N1 viruses containing the NA of A/California/07/09(H1N1)pdm, А/New Caledonia/20/1999(H1N1) or A/Vietnam/1203/04(H5N1), and the hemagglutinin derived from A/equine/Prague/1/56(H7N7) influenza virus. Adjustment and validation of the NI test with the reassortant viruses included the determination of the viruses working concentration and selection of optimal conditions for the enzymatic reaction.</p>

[BEFORE_START]
<p><em>Preparation of reagents dilutions</em></p>
<ol>
<li>Prepare a stock solution of fetuin at 10 mg/ml in coating buffer and store in 500 µl aliquots at -20 °C.</li>
<li>Prepare a working solution of fetuin (50 µg/ml) immediately before coating plates by diluting the stock solution 200-fold in coating buffer.</li>
<li>Prepare a stock solution of BSA at 100 mg/ml in sterile PBS (sPBS) and store in 10-25 ml aliquots at -20°C.</li>
<li>The diluent for virus and sample dilutions (PBS-BSA). Prepare the 1% work solution of BSA (10 mg/ml) right before assay using PBS as a diluent. After thawing aliquot with stock BSA, you may storage (1-2 months) it in a refrigerator at +4°C for next assays.</li>
<li>To prepare a stock solution of lectin, mix 1 mg of lectin and 5 ml of sPBS and store at +4°C.</li>
<li>Right before use, prepare a work solution of lectin at 2.5 μg/ml by diluting the stock solution 80-fold in PBS-BSA.</li>
<li>Prepare a work solution of RDE from original solution by 10-fold dilution at PBS-BSA.</li>
<li>Prepare a sufficient volume of wash buffer – 0.5% PBS-Tween 20 (T-PBS). Store at +4°C.</li>
<li>Prepare the peroxidase substrate TMB. Place at room temperature for 30 min before use to warm up.</li>
<li>Prepare the stop solution - 1 N H<sub>2</sub>SO<sub>4</sub> - add 27.2 ml stock 98% H<sub>2</sub>SO<sub>4</sub> to 973 ml distilled H<sub>2</sub>O.</li>
</ol>
<p><em>Serum samples </em></p>
<ol>
<li>Heat all sera in a water bath at 56°C for 30 min. Store the sera at -20°C before and after the heat treatment.</li>
</ol>

[STEPS]
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TITLE: Yale - Non-Esterified Fatty Acids
AUTHORS: John Stack, Gary Cline

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span style = "font-weight:bold;">Summary: </span></div><div class = "text-block">Procedure used to determine the concentration of NEFA in blood, serum, and plasma. NEFA; Free fatty acids are measured in a multistep reaction to form an colored adduct of 3-methyl-Nethyl-N-(b-hydroxy-ethyl)-analine and 4-aminoantipyridine monitored at 560 nm.</div></div>

[STEPS]
?. Calibrate Cobas for NEFA analysis by running a NEFA standard.
?. Sample handling as performed by the Cobas Mira Plus    a) Pipette 6µL of sample into cuvette.    b) Add 225 µL of NEFA Reagent A Mixture.    c) Add 75 µL of NEFA Reagent B Mixture.    d) Mixture is incubated at 37ºC for 10 minutes    e) Absorbance is measured at 560 nm.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>We conducted fluorimetric assays for each of three hydrolytic enzymes: b-glucosidase and cellobiohydrolase, and<em> N</em>-acetyl-glucosaminidase in black microplates. We measured fluorescence at 365 nm excitation and 450 nm emission. From each sample, we recorded fluorescence values for MUB substrate (substrate control), homogenate (homogenate control), MUB standards in the presence of maleate buffer (standard), and MUB in the presence of homogenate. We calculated potential extracellular enzyme activity of NAG, BG, and CBH from each litter sample.  </p>

[BEFORE_START]
<p><strong>Reagents:</strong></p>
<p> </p>
<p><strong>1.0 M NaOH</strong></p>
<p>4 g NaOH pellets; 100 mL DI water</p>
<p> </p>
<p><strong>50 mM sodium acetate buffer, pH 5.0 </strong>(can make a 10X stock solution; pH can vary with soil)</p>
<p>4.374 g sodium acetate trihydrate </p>
<p>1.1 ml glacial acetic acid (add more to make pH = 5) </p>
<p>1 L sterile DI water (miliQ water)</p>
<p> </p>
<p><strong>Substrate solutions (1000 μM unless otherwise noted):</strong></p>
<table width="464">
<tbody>
<tr>
<td width="232">
<p>Enzyme</p>
</td>
<td width="232">
<p>Substrate</p>
</td>
</tr>
<tr>
<td width="232">
<p>Cellobiohydrolase (CBH)</p>
</td>
<td width="232">
<p>4-MUB-β-D-cellobioside (in refrigerator) 12.5 mg/50 mL <strong>500 μM</strong></p>
</td>
</tr>
<tr>
<td width="232">
<p>β-Glucosidase (BG)</p>
<p> </p>
</td>
<td width="232">
<p>4-Methylumbelliferyl β-D-glucopyranoside 16.9 mg/50 mL <strong>1000 μM</strong></p>
<p> </p>
</td>
</tr>
<tr>
<td width="232">
<p>α-Glucosidase (AG)</p>
<p> </p>
</td>
<td width="232">
<p>4-Methylumbelliferyl α-D-glucopyranoside 16.9 mg/50 mL <strong>1000 μM</strong></p>
<p> </p>
</td>
</tr>
<tr>
<td width="232">
<p>β-xylosidase (BX)</p>
</td>
<td width="232">
<p>4-Methylumbelliferyl β-D-xylopyranoside 15.4 mg/50 mL <strong>1000 μM</strong></p>
</td>
</tr>
<tr>
<td width="232">
<p>MUB standard</p>
<p> </p>
</td>
<td width="232">
<p>1000 μM 4-Methylumbelliferone 0.89 mg/50 ml <strong>100 μM</strong></p>
</td>
</tr>
</tbody>
</table>
<p> </p>
<p>Make substrate and fluorescent standard solutions in 125 mL red glass bottles using milliQ water. Store solutions in the 4°C refrigerator. Substrates are in the freezer unless noted. Remake solutions every week, and make them one day BEFORE you want to start working, as this affects the measured activity levels. MUB standard should be diluted to 25 μM before use, depending on expected sample fluorescence. MUB is made by heating the solution to 90°C for a few minutes, with constant stirring (don’t let it boil).</p>

[STEPS]
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TITLE: 10x Protocols: Visium v2 CytAssist FFPE Library Construction -- University of Minnesota TMCs (CG000495 Rev A)
AUTHORS: IOx Genomics, Laura J Niedernhofer, David A Bernlohr

[DESCRIPTION]
Protocols from 10x Genomics for Visium Spatial Gene Expression v2 chemistry on FPPE samples with the CytAssist component.

Protocol ID# (CG) and Revision used: 
    10x protocol CG000495, Revision A -- Library Construction with CytAssist

Note: These protocols may not be the current version offered by 10x Genomics but were used to produce the specific datasets connected to them. Please review the 10x Genomics support website for the most recent versions of the protocols prior to starting your experiment. 

[STEPS]
1. 10x protocol CG000 495, Revision A (Library construction with CytAssist)
  

2. Additional Protocols/Resources
https://www.10xgenomics.com/support/spatial-gene-expression-ffpe