TITLE: Sample run SOP for ECD/FID/TCD
AUTHORS: Sparky Jr.

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">Sample run SOP for ECD/FID/TCD by Sparky Jr.</div></div>

[STEPS]
?. [Startup]
Open HP ChemStation using the shortcut on the desktop
?. [Startup]
Start each instrument listed in the Utilities menu:SparkyJrECD/FID
?. [Sample Run]
To run samples on the ECD/FID GC with HP 7694 Headspace Autosampler (Sparky Jr.):
?. [Sample Run]
Create a sequenceSequence ParametersSequence OutputSequence SummarySequence Table
?. [Sample Run]
Queue the sequence
?. [Sample Run]
Set vial parameters on HP7694 (Sparky Jr.) and start run
?. [Sequence Setup]
Set up a sequence for each instrument. Verify that the settings for the following sequence related items are as shown in the SOP.Sequence ParametersSequence OutputSequence SummarySequence Table
?. [Sequence Parameters for FIDTCD]
Set the Sequence Parameters as shown below. Other than the Subdirectory, these are the default settings, and should not need to be changed for each run.Operator Name: “your initials”(Ex. MGD)Data File: Prefix/CounterSignal 1:        Prefix: SIG1        Counter: 0001Subdirectory: run date (MMDDYYA...Z). HP Chem will automatically create the subdirectory if it does not already exist.Path: C:\HPCHEM\1\DATA (This can only be changed in the HP Chemstation Utilities tool.)Part of methods to run: According to Runtime ChecklistSequence Comment: Optional comment regarding samples or sequence, etc.  For ECDFID the parameters are the same except:Operator Name: “your initials” (Ex. MGD)Data file -- Path: C:\HPCHEM\2\DATA        Prefix: SIG1        Counter: 0001Subdirectory: run date (MMDDYYA...Z). HP Chem will automatically create the subdirectory if it does not already exist.Path: C:\HPCHEM\1\DATA (This can only be changed in the HP Chemstation Utilities tool.)Part of methods to run: According to Runtime ChecklistSequence Comment: Optional comment regarding samples or sequence, etc.  For ECDFID the parameters are the same except:Operator Name: “your initials” (Ex. MGD)Data file -- Path: C:\HPCHEM\2\DATAFor ECDFID the parameters are the same except:Operator Name: “your initials” (Ex. MGD)Data file -- Path: C:\HPCHEM\2\DATA
?. [Sequence Output]
Sequence Summary            Print Sequence Summary Report:                    Report to File                            Signal 1: FIDTCD.TXT for FIDTCD                            Signal 1: ECDFID.TXT for ECD/FID GC            Print individual reports for each run as wellDestination of individual reports for each run:            Print Sequence Summary Report:                    Report to File                            Signal 1: FIDTCD.TXT for FIDTCD                            Signal 1: ECDFID.TXT for ECD/FID GC            Print individual reports for each run as well            as specified in each MethodThe individual reports will be saved in each data folder SIG1XXXX.
?. [Sequence Summary or Setup in Sequence Output]
Activate report:8. Statistics sample runs [Standard Statistics]9. Summary [Compound Summary]
?. [Sequence Table]
Use Insert Vial Range to create a Sequence Table.Create matching Sparky Jr. and ECD/FID GC sequence tables with the same number of samples and corresponding Method Name.Method Name        FID for Sparky Jr.        ECD for ECD/FID GCStart VialEnd VialInj/Vial:1        FID for Sparky Jr.        ECD for ECD/FID GCStart VialEnd VialInj/Vial:1
?. [Sequence Table]
Enter sample ID, vial number etc. in Sample Name column of ECD/FID GC sequence table. Whenever possible give some indication of what data set the vials belong to in the first and last sample name for the dataset. For example, 4001 MMDD indicates that the sample run starting with vial# 01 is from the MMDD sampling of incubation Set BC 40. It is not necessary to enter this information in the Sparky Jr. sequence table.
?. [Sequence Table]
Save each sequence table as the run date (MMDDYYA...Z) to match Sequence Parameters Subdirectory.
?. [Waiting for Injection]
Return to each sequence table and click on Run Sequenceto queue sequence tables and send methods to Sparky Jr. and ECD/FID GC.
?. [Waiting for Injection]
Once methods have been loaded and sequences queued, HP Chem will display Waiting for Injection screen.Verify that the Method, Sequence Table, Inj/Vial, and Data File are correct.
?. [HP7694]
Set Vial Parameters to corresponding first and last vial
?. [HP7694]
When ECD/FID GC and Sparky Jr. are Waiting for Injection, press Start/Stop button to begin sample run.
?. [Running more than 44 vials in a single sequence table]
HP Chem will remain in Waiting for Injection state if the sequence table has more samples than were queued into the headspace autosampler (HP7694). This allows us to run more than 44 vials within the same sequence table.For example, to run 74 vials:
?. [Running more than 44 vials in a single sequence table]
Create a sequence table with 74 entries.
?. [Running more than 44 vials in a single sequence table]
Set the headspace autosampler to run vials 1 through 30.
?. [Running more than 44 vials in a single sequence table]
When the sequence table gets to sample 31, it will wait for the start signal from the autosampler.
?. [Running more than 44 vials in a single sequence table]
Reload the autosampler and set it to run vials 1 through 44.
?. [Running more than 44 vials in a single sequence table]
Press Start/Stop to continue the run. The system will now run the remaining 44 sample vials.
?. [Partial Sequence]
?. [Reprocess Samples]
To reprocess files when sequence was NOT queued:
?. [Reprocess Samples]
Copy data folders into target subdirectory
?. [Reprocess Samples]
Edit Sequence Parameters:Sig Prefix & Counter to watch first data folder in groupPart of Method: Reprocess Only
?. [Reprocess Samples]
Make sure sequence has correct number of samples/names
?. [Reprocess Samples]
Run Sequence