TITLE: Assembly Instructions for colosseum
AUTHORS: Sina Booeshaghi, Yeokyoung Kil, Kyung Hoi Min, Jase Gehring, Lior Pachter

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block"><span>We present colosseum, a low-cost, modular, and automated fluid sampling device for scalable fluidic applications. The colosseum fraction collector uses a single motor, can be built for less than $100 using off-the-shelf and 3D-printed components, and can be assembled in less than an hour. Build Instructions and source files are available at </span><a href="https://github.com/pachterlab/colosseum" style = "text-decoration:underline;color:blue;cursor:pointer;"><span style = ":;:UNDERLINE;">https://github.com/pachterlab/colosseum</span></a><span style = ":;:UNDERLINE;">.</span></div></div>

[STEPS]
?. [Preparation]
Prepare 3D-printed components and parts.
?. [Base and Base Plate Assembly]
Assemble the base and motor.
?. [Base and Base Plate Assembly]
Insert M5 screws into the narrower ends of the rubber feet. Two M5 screws should be 20 mm long, and the other three screws should be shorter (16 mm works fine).
?. [Base and Base Plate Assembly]
Turn the base right side up and place two M5 hex nuts in the rectangular slots in the rectangular part of the base. The nuts should just sit in place and secure the rubber feet with the two longer M5 screws in place.
?. [Base and Base Plate Assembly]
Screw the rubber feet onto the bottom side of the base. The holes in the circular part of the base should get the rubber feet with the shorter M5 screws. Hold the M5 hex nuts in place as you screw in the two rubber feet with the 20 mm M5 screws.
?. [Base and Base Plate Assembly]
Place the motor upside down on the floor of the base, aligning the four holes of the motor with the four elliptical slits in the base. Pull the wires out through the openings on the wall of the base.
?. [Base and Base Plate Assembly]
Loosely secure the motor in place with four M3 hex screws and washers. Make sure to place the washers between the plastic of the base and the head of the M3 hex screws so that the plastic does not get worn out. We will need to move the motor later so do not tighten the M3 screws all the way.
?. [Base and Base Plate Assembly]
Add the motor coupling pulley to the shaft of the motor. The side with the teeth should be facing towards the base. Secure it in place with the set screws.
?. [Base and Base Plate Assembly]
Take the 6 mm-bore coupling pulley and wrap the 120 mm pulley belt around both pulleys. Refer to the picture. Then, in the 6 mm-bore pulley, insert the shaft. Push the shaft all the way through the pulley so that the bottom of the shaft is flushed with the bottom of the pulley, and secure in place with set screws.
?. [Base and Base Plate Assembly]
Insert two bigger ball bearings into the circular slots on the floor of the base.
?. [Base and Base Plate Assembly]
Insert the 3D-printed base plate into the base. Then, place two bigger ball bearings like you did for the floor of the base.
?. [Base and Base Plate Assembly]
Secure the base plate in place with three long M5 screws and hex nuts.
?. [Base and Base Plate Assembly]
Now, sliding the motor away from the shaft so that the pulley belt is taut, secure the motor tightly in place by tightening the four M3 screws. IMPORTANT: Make sure you are not pulling too hard that the shaft is tilted.
?. [Base and Base Plate Assembly]
Insert three small ball bearings into the rectangular slots on the top rim of the base. This is to ensure that the tube rack rotates with little friction. Secure the small ball bearings in place with M5 dowels.The base and base plate are now finished!
?. [Arm Assembly]
Insert an M3 hex nut into the rectangular slot on the side of the arm. Then, screw an M3 hex screw into the circular hole that goes through the hex nut.
?. [Arm Assembly]
Push a small ball bearing through the little nib on the bottom arm. If the ball bearing does not go in, squeeze the nib with pliers. You may need to use some force on the bearing.
?. [Arm Assembly]
Slide the rotary shaft into the arm and secure with the M3 set screw we inserted earlier. Then, add a shaft collar at the bottom of the arm so that it adds to the height of the arm.Arm assembly is complete!
?. [Tube Rack Assembly]
Take the flange coupling and insert four M3 hex screws into the flat side of the coupling.
?. [Tube Rack Assembly]
Put the flange coupling into the cylindrical hole of the tube rack so that the protruded part of the flange coupling sticks out from the top of the tube rack. Screw the M3 hex screws all the way in.
?. [Tube Rack Assembly]
Add the set screw on the flange coupling and set loosely in place.
?. [Tube Rack Assembly]
Tighten the M3 screws in place with M3 hex nuts.Tube rack assembly is complete!
?. [Complete Assembly]
Take the arm and tube rack and hold them as shown in the picture. This is the easiest way to put everything together. Then, put the parts together by inserting the base rotary shaft into the tube rack and putting the arm rotary shaft through the ball bearing in the rectangular part of the base.
?. [Complete Assembly]
Finally, tighten the set screws on the flange coupling and we are done assembling colosseum.