TITLE: Field sampling of root-associated microbes for DNA/RNA extraction
AUTHORS: Roey Angel

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol describes a procedure for sampling plant roots in the field for future DNA and RNA extraction for microbiome analysis. The protocol is deliberately designed to be simple and requires no electronic equipment. Root samples are preserved in LifeGuard Soil Preservation Solution for protecting against nucleic acid degradation.</div></div>

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Albanian
Arabic
Armenian
Azerbaijani
Basque
Belarusian
Bengali
Bosnian
Bulgarian
Catalan
Cebuano
Chichewa
Chinese (Simplified)
Chinese (Traditional)
Croatian
Czech
Danish
Dutch
English
Esperanto
Estonian
Filipino
Finnish
French
Galician
Georgian
German
Greek
Gujarati
Haitian Creole
Hausa
Hebrew
Hindi
Hmong
Hungarian
Icelandic
Igbo
Indonesian
Irish
Italian
Japanese
Javanese
Kannada
Kazakh
Khmer
Korean
Lao
Latin
Latvian
Lithuanian
Macedonian
Malagasy
Malay
Malayalam
Maltese
Maori
Marathi
Mongolian
Myanmar (Burmese)
Nepali
Norwegian
Persian
Polish
Portuguese
Punjabi
Romanian
Russian
Serbian
Sesotho
Sinhala
Slovak
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?. 2 g
The root tissue should make up at least half or more of the mass, while the remaining attached soil should make up the rest
?. Make sure the roots do not take up more than 1/2-2/3 of the volumeIt is of course possible to split each sample into several separate tubes, depending on the specific type of roots, and submerge each with LifeGuard solution
?. 1.5 ml
?. 4 °C
?. [or -80 °C]