eamag/protocols-with-steps huggingface dataset:
TITLE: No Title
AUTHORS: 

[GUIDELINES]
<p><strong>Tips</strong>:</p>
<p><strong>High background</strong></p>
<p>1. Transfer buffers may have become contaminated. Contamination can be transferred to the blots from electrophoresis and related equipment used in blot preparation.</p>
<p>2. Post-antibody washes may not have been performed for a sufficient period of time or were not performed in a high enough volume.</p>
<p>3. The blocking and incubation agents used were not freshly prepared or were too dilute.</p>
<p> </p>
<p><strong>No signal or poor signal</strong></p>
<p>1. Transfer efficiency may have been poor. Check protein transfer by staining the gel and/or membrane.</p>
<p>2. Incorrect storage of antibodies or ECL western blotting detection reagents may result in a loss of signal.</p>
<p>3. Insufficient protein may have been loaded on the gel. Depending on the location of the target protein, membrane or nuclear preparations may be required (instead of whole cell lysates).</p>
<p>4. Film exposure time may have been too short.</p>
<p> </p>
<p><strong>Solutions and Reagents:</strong></p>
<p> </p>
<p><strong>1X Cell Lysis Buffer: </strong></p>
<p>20 mM Tris-HCl, pH 7.5</p>
<p>150 mM NaCl</p>
<p>1% NP-40</p>
<p>2 mM EDTA</p>
<p>1 µg/ml leupeptin</p>
<p>1 µg/ml aprotinin</p>
<p>1 mM Na<sub>3</sub>PO<sub>4</sub></p>
<p>1 mM PMSF</p>
<p>5 mM NaF</p>
<p>3 mM Na<sub>4</sub>P<sub>2</sub>O<sub>4</sub></p>
<p> </p>
<p><strong>5X SDS Sample Buffer:</strong></p>
<p>312.5 mM Tris-HCl (pH 6.8)</p>
<p>10% SDS (w/v)</p>
<p>250 mM DTT</p>
<p>50% Glycerol</p>
<p>0.05% Bromophenol Blue (w/v)</p>
<p>Use at 1X</p>
<p> </p>
<p><strong>10X SDS Running Buffer</strong></p>
<p>Dissolve 144 g of Glycine, 30 g of Tris base and 10 g SDS in 800 ml of distilled H2O.</p>
<p>Add distilled H2O to 1 liter</p>
<p>Use at 1X</p>
<p> </p>
<p><strong>Transfer Buffer:</strong></p>
<p>3.0 g Tris base<br />14.4 g Glycine<br />200 ml Methanol<br />Add deionized water to 1.0 L</p>
<p> </p>
<p><strong>10X TBS-T (Tris-buffered saline containing Tween-20):</strong></p>
<p>Dissolve 80 g of NaCl, 2 g of KCl, 30 g of Tris base and 10 ml Tween-20 in 800 ml of distilled H<sub>2</sub>O.</p>
<p>Adjust the pH to 7.4 with HCl. Add distilled H2O to 1 liter.</p>
<p>Use at 1X (containing 0.1% Tween-20).</p>
<p> </p>
<p><strong>Blocking Buffer:</strong></p>
<p>1X TBS-T with 5% nonfat dry milk</p>
<p> </p>
<p><strong>Wash Buffer:</strong></p>
<p>1X TBS-T</p>
<p> </p>
<p><strong>Primary and Secondary Antibody Dilution Buffer:</strong></p>
<p>1X TBS-T with 5% nonfat dry milk</p>
<p> </p>
<p>**If phosphorylation-specific antibodies are used, the membrane blocking buffer and antibody dilution buffer should not contain milk.</p>
<p> </p>
<p><strong>Alternate Blocking Buffer:</strong></p>
<p>1X TBS-T with 4% Bovine Serum Albumin (BSA)</p>
<p> </p>
<p><strong>Alternate Primary and Secondary Antibody Dilution Buffer:</strong></p>
<p>1X TBS-T with 4% Bovine Serum Albumin (BSA)</p>
<p> </p>
<p><strong>Blotting Membrane:</strong></p>
<p>Nitrocellulose or PVDF membrane</p>

[STEPS]
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TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This protocol describes MesoScale Discovery (MSD) assays  for measurement of proinflammatory cytokines in supernatants collected from T cell dependent cytotoxicity (TDCC) cultures after BiTE® treatment.</p>

[STEPS]
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TITLE: Tail Suspension Test
AUTHORS: Marina Lorente Picón, Núria Peñuelas, Ariadna Laguna, Miquel Vila

[DESCRIPTION]
Tail Suspension Test for mice

[STEPS]
1. Suspend animals by their tails with tape in a suspension bar.
2. To avoid the tail climbing behavior, pass a 2cm methacrylate tube through the tail before suspending the animal. 
3. Quantify the escape-oriented behaviors (i.e. fore and hind limbs movement) during six minutes. 
4. Calculate the total immobilization time (s) as the sum of all the time the animal was not performing any escape-oriented behavior.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<div>
<p>This protocol was designed and developed at this laboratory.</p>
<p>The protocol specifically aims to amplify strains of Influenza B VICTORIA virus lineage and not strains of the YAMAGATA virus lineage or other virus species. The assay targets the haemagglutinin (HA) region and is designed as a qualitative lineage-typing test for human cases of seasonal influenza virus type B infections.</p>
<p>FluB-VICT-TM2018 is ideally used alongside its companion protocol, 'Influenza B virus YAMAGATA lineage TaqMan 2018' (FluB-YAMA-TM2018), which aims to target influenza B virus YAMAGATA lineage strains exclusively. The two assays perform best as UNIPLEX protocols; a drop in sensitivity was observed when combined in a DUPLEX format.</p>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>

[BEFORE_START]
<div>
<div>
<div>
<div>
<div>
<div>
<ul>
<li>If using a different brand or model of real-time thermocycler, check the concentration of ROX is adequate.</li>
<li>Method assumes the user is familiar with the thermocycler and software used to run the protocol and with PCR in general.</li>
</ul>
</div>
</div>
</div>
</div>
</div>
</div>

[STEPS]
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TITLE: Keto Extreme Fat Burner South Africa 2022: The Truth Revealed by Customers
AUTHORS: health  

[DESCRIPTION]
Keto Extreme Fat Burner South Africa is a weight loss supplement that  claims to be the most powerful and effective fat burner on the market.  It’s made from natural ingredients clinically studied and proven to help  you lose weight fast. Keto Extreme Fat Burner is available in three  different packages.

It is worth the money because it works properly as claimed by the  manufacturer. The supplement is clinically studied and proven to help  you lose weight fast. Keto Extreme Fat Burner is available in three  different packages, including a three-month supply and a six-month  supply package.

>>>Click to Buy — Purchase Only From Official Website

>>>Click to Buy — Purchase Only From Official Website

[STEPS]
TITLE: No Title
AUTHORS: 

[GUIDELINES]
(using GIBCO LM agarose = now called Invitrogen Life Technologies LMP agarose, 1-800-955-6288, cat# 1-5517-014 for 50 g, 1-5517-022 for 100 g)<br /><strong>Note:</strong> new catalog number is16520-050.

[STEPS]
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TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>This protocol explains how to prepare, load and run cell-free RNAPT7 transcription/translation coupled reactions. To be able to follow this protocol it is necessary to have prepared all the solutions needed including <strong>amino acids and Energy solutions</strong> and <strong>cell extracts (</strong> explained in detail in Step 1 and Step 2 of this collection).</p>
<p> </p>

[GUIDELINES]
<p>  </p>
<p> </p>

[STEPS]
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TITLE: test publish protocol 
AUTHORS: Maria Guliakina

[STEPS]
?. Village did removed enjoyed explain nor ham saw   calling talking. Securing as informed declared or margaret. Joy horrible moreover man feelings own shy. Request norland neither mistake for yet. Between the for morning assured country believe. On even feet time have an no at. Relation so in confined smallest children unpacked delicate. Why sir end believe uncivil respect. Always get adieus nature day course for common. My little garret repair to desire he esteem. test test    In it except to so temper mutual tastes mother. Interested cultivated its continuing now yet are. Out interested acceptance our partiality affronting unpleasant why add. Esteem garden men yet shy course. Consulted up my tolerably sometimes perpetual oh. Expression acceptance imprudence particular had eat unsatiable.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p><strong>This is a short tutorial on how to get started with gene prediciton in Prokaryotic genomes via the command line.</strong></p>
<p> </p>
<p>Code is intended for use on an Ubuntu 16.04 LTS OS.</p>
<p> </p>
<p>The tools we will use here are:</p>
<p>Prodigal. https://github.com/hyattpd/Prodigal</p>
<p>Barrnap. https://github.com/tseemann/barrnap</p>
<p> </p>
<p>This tutorial is intended for teaching purposes, but if you use any of the tools in a scientific paper do not forget to cite the appropriate publications!</p>
<p> </p>
<p><strong>Prodigal:</strong></p>
<p>Prodigal: prokaryotic gene recognition and translation initiation site identification.</p>
<p>BMC Bioinformatics, 2010, Volume 11, Number 1, Page 1</p>
<div>Doug Hyatt, Gwo-Liang Chen, Philip F LoCascio, <span style="display: inline;"> Miriam L Land, </span><span style="display: inline;"> Frank W Larimer, </span><span style="display: inline;"> Loren J Hauser </span></div>
<p> </p>
<p><strong>Barrnap:</strong></p>
<p>Seemann T (2013), barrnap 0.8 : rap. id ribosomal RNA predictionhttps://github.com/tseemann/barrnap</p>
<p> </p>
<p><strong>Bedtools</strong></p>
<div>
<div>BEDTools: a flexible suite of utilities for comparing genomic features.<em> Bioinformatics</em>, Volume 26, Issue 6, 15 March 2010, Pages 841–842, <a href="https://doi.org/10.1093/bioinformatics/btq033" target="_blank" rel="noopener noreferrer">https://doi.org/10.1093/bioinformatics/btq033</a></div>
</div>

[STEPS]
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TITLE: Stereology
AUTHORS: Haley   Geertsma

[DESCRIPTION]
This protocol is used to quantify the number of TH+ cells in the substantia nigra pars compacta.

[STEPS]
1. Take 8 cryosections cut at 40μm to equally sample the substantia nigra, pars compacta (SNc) and perform diaminobenzidine staining with a tyrosine hydroxylase antibody.
Note: I take every 6th section from Bregma -2.54mm to -3.88mm
2. Use StereoInvestigator software to count all TH+ cells. For this, randomly sample the SNc with a 150μm x 150μm grid and apply an optical fractionator consisting of a 75μm x 75μm square. Calculate the mean section thickness during counting with a frequency of 10 frames (~3 measurements per hemisphere).
TITLE: Co-crystallisation protocol for SARS-CoV-2 nsp3 macrodomain in P1 21 1 with ligands
AUTHORS: Jasmin Aschenbrenner, Peter Marples, Daren Fearon

[DESCRIPTION]
The COVID-19 pandemic has demonstrated the need for novel therapeutic interventions and improved pandemic preparedness strategies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This protocol details an optimized crystallization method for the SARS-CoV-2 nsp3 macrodomain, a potential drug target. Using sitting drop vapor diffusion with seeding, we describe specific buffer conditions and procedures to produce high-quality crystals of ligand-bound nsp3-mac.
All structures solved during the development of tool compounds for the SARS-CoV-2 nsp3 macrodomain are deposited on the PDB (Group deposition: G_1002283).

[STEPS]
SECTION: Crystallization experiment
4. Protein, compounds and buffer requirements:
115.2 µL21.6 mg/mL  
2.88 mL 
28.8 µL   seeds, dilution 1:2
12 µL100 millimolar (mM)   ligand in DMSO
SECTION: Crystallization experiment
6. Dispense 30 µL into SwissCI 3 lens plate reservoir wells using a 100 µl multi-channel pipette.
Dispense 40 nL Compound (~ 5% of final drop volume) to each lens using the Echo liquid handler.
Dispense 400 nL21.6 mg/mL    to each lens using the SPT mosquito.
Dispense  300 nL to each lens using the SPT mosquito.
Dispense  100 nL to each lens using the SPT mosquito.

Drop ratio: 400 nL   : 300 nL reservoir solution: 100 nL seeds: 40 nL compounds
Final drop volume: 840 nL
SECTION: Crystallization experiment
5. Crystallisation screen composition:
0.1 Molarity (M)  MES pH 6.5 
30% w/v PEG 3000

Stock solutions used:
1 Molarity (M)  MES adjusted to pH 6.5 with HCl
50% w/v PEG 3000

 

SECTION: Crystallization experiment
8. Crystal form after ~12 h.
 


SECTION: Crystallization experiment
7. Incubate at 20 °C for 1440 min to maximum of 2880 min  in Formulatrix Rock Imager.

Imaging Schedule: The first images are taken after 12 h and the imaging schedule follows a Fibonacci sequence of days for further collections. 
SECTION: Equipment needed
2. Echo liquid handler
Formulatrix Rock Imager (or incubator of choice)
SPT mosquito
 
P100 8 multi-channel pipette
SwissCI 3 lens plate

SECTION: Crystallization experiment
3. Prepare seed stock:
 
1: 2 dilution    seeds
SECTION: SARS-CoV-2 nsp3 macrodomain expression and purification
1. The protein used for crystallisation was expressed and purified using the following protocol.
 

SECTION: Data Collection at Synchrotron
9. Diamond Light Source
Unattended Data Collection (UDC)
Data Collection Temperature: 100K
Detector: DECTRIS EIGER2 X 9M
Beamline: I04-1
Wavelength:  0.9212 Å
Resolution (Å): 1.62
Beam Size (μm): 60 X 50
Number of images: 3600
Oscillation: 0.10°
Exposure (s): 0.0020
Transmission (%): 100
Flux (ph/s): 3.80e+12
TITLE: Deleidi Lab | Protocols | Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism
AUTHORS: michela.deleidi, María José Pérez J., Hariam Raji, Federico   Bertoli, Pascale Baden

[DESCRIPTION]
Collection of protocols of Team Deleidi used in the publication: "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism"

[STEPS]
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
<p>Protocol to extract the inner membranes from yeast cells</p>

[STEPS]
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TITLE: Preparation of Holding ACSF (artificial cerebrospinal fluid) for slice electrophysiology
AUTHORS: Sarthak M. Singhal, thnasko

[DESCRIPTION]
Hnasko lab- preparation of Holding ACSF
Holding ACSF is used for incubating brain slices prior to cell-attached or whole-cell electrophysiological recordings of neurons.

[STEPS]
1. Prepare 10X stock solution.
1.1. Composition of 1 L of 10X Holding ACSF (in mM): 1150 NaCl, 25 KCl, 12.3 NaH2PO4, 100 d-glucose, 20 CaCl2 and 20 MgSO4.

1.2. Composition of 1 L of 10X Holding ACSF (in g): 67.2 NaCl, 1.86 KCl, 1.47 NaH2PO4, 18.02 d-glucose, 2.94 CaCl2 and 4.93 MgSO4.
2. Dissolve all salts in 1 L of ddH2O to make 10X stock solution of Holding ACSF.

3. Dilute 10X solution to 1X for use in experiments.
3.1. Dilute 100 mL of 10X solution to ~ 800 mL with ddH2O and add (in mM): 26 NaHCO3, 2 thiourea, 5 Na-ascorbate and 3 Na-pyruvate; (in g): 2.18 NaHCO3, 0.15 thiourea, 0.98 Na-ascorbate and 0.33 Na-pyruvate. 
3.2. Add ddH2O to final volume of 1 L.

4. Bubble the solution with carbogen (95% O2 5% CO2) for ~10 minutes.

5. After bubbling, measure and adjust pH of solution to ~ pH 7.3 with 12 N HCl (<1 ml).

6. Refrigerate until use.
TITLE: In vivo Ephys: Analysis Protocol
AUTHORS: Sasha Burwell

[DESCRIPTION]
This protocol details the analysis procedures performed on the in vivo electrophysiology data post-spike sorting.

[STEPS]
SECTION: Analysis Protocol
1. To plot the waveforms from one electrode of interest in the raw data (ex, Fig. 1c), run the Matlab code 
 
 on the raw continuous data.
SECTION: Analysis Protocol
2. To analyze the sorted templates extracted from Spyking Circus, 
SECTION: Analysis Protocol
3. From the resulting Templates_data structure, save each row as an individual dat variable.

i.e., for ch 15 in the above example:

 

SECTION: Analysis Protocol
4. Copy multiple channels into one folder for grouped analysis, and run 
 
 in this folder.
SECTION: Analysis Protocol
5. Referencing the control and experimental 
 
 data structures created from 
 
, run 
 
 to generate the plots seen in the left of Fig. 1d, e, and f.
SECTION: Analysis Protocol
2.1. Use the custom matlab code.
 

SECTION: Analysis Protocol
2.2. chWithCell = array of the electrode which has a cell for each template (ex [5 15 3]).

 

SECTION: Analysis Protocol
2.3. Calculates and plots spike amplitude, cumulative spikes, and firing rate in Hz for all the templates saved.
SECTION: Analysis Protocol
6. Use the features in the 
 
 data structure:
SECTION: Analysis Protocol
6.1. Calculate delta norm as (post-pre)/(post+pre) for each feature for each cell.
SECTION: Analysis Protocol
6.2. Input ddHTP values as 
 
 and +HTP values as 
 
 into the Matlab code 

 
 for statistical analysis and generating the plots seen in the right of Fib. 1d, e, and f.
SECTION: Analysis Protocol
6.3. Use GraphPad Prism to generate the plots in Ext. Fig 2d and h-l from the delta norm features.
TITLE: Tension Between Polynesians and French of Tahiti and Mo&#39;orea Protocols
AUTHORS: Sage Moloney

[DESCRIPTION]
This research project will seek to answer the question: What are the sources of tension between native Polynesians and individuals from France on Tahiti and Moorea? In order to answer this question, I will be surveying individuals that identify as native Polynesians, as well as individuals that have moved to Polynesia from France.

[STEPS]
SECTION: Preparing the Questionnaire
1. Develop questions that will reveal the perceptions of tension between Polynesians and the French from the perspective of the respondent.
SECTION: Preparing the Questionnaire
2. Prior to entering the field, this questionnaire should be revised and reviewed for potential sources of bias and overall efficacy.
SECTION: Finding Survey Participants
3. Surveys will be conducted orally, and researchers will be stationed in public places across Mo'orea and Tahiti, asking individuals if they would be interested in participating in a short research survey.
SECTION: Finding Survey Participants
3.1. Researchers will be stationed in both rural and urban areas, as well as higher-income and lower-income communities in order to increase the diversity of responses.
SECTION: Finding Survey Participants
3.2. Survey data will remain anonymous, however economic information and occupation will remain tied to an individual's responses in order to accurately assess data.
SECTION: Conducting the Survey
4. Researcher will conduct the questionnaire as written.
SECTION: Conducting the Survey
5. At the conclusion of the questionnaire, researcher will assess:
SECTION: Conducting the Survey
5.1. Does the participant feel there is significant tension between Polynesians and the French?
SECTION: Conducting the Survey
5.2. According to the participant, what are the main sources of this tension?
SECTION: Analyzing the Data
6. Data will then be compiled and analyzed for repeating themes, including frequently mentioned sources of tension by any particular occupation or economic group and the relationship between occupation and economic class and a participant's opinion regarding whether tension exists.
SECTION: Analyzing the Data
7. Data will be supplemented by an historical analysis of the situation in French Polynesia and a quantitative analysis will be conducted regarding what historical events may have led to current tension.
TITLE:  Universal sandwich ELISA for investigating the binding of Protein-LA (SpLA) to avian immunoglobulins  using a peroxidase-labeled -anti-IgY conjugate.
AUTHORS: Angel  Justiz-Vaillant, Monica F. Smikle

[DESCRIPTION]
<div class = "text-blocks"></div>

[STEPS]
?. This ELISA is used to study the interaction of protein-LA (SpLA) with diverse avian immunoglobulins.
?. The 96 well microtitre plate is coated overnight at 4°C with 2 µg/µl per well of recombinant SpLA or a mixture of SpA with SpL in carbonate-bicarbonate buffer pH 9.6.
?. Then plate is treated with bovine serum albumin solution and washed 4X with PBS-Tween.
?. 50 µl of  avian egg yolk or egg white (1 mg/ml) is added and incubated for 1.30h at RT and the microplate is then rewashed 4X with PBS-Tween.
?. Then 50 µl of peroxidase-labeled-anti-IgY conjugate diluted 1:15000 in PBS-non-fat milk is added to each well and  incubated for 1.30h at RT. After that the plate is washed 4X with PBS-Tween.
?. Pipette 50 μl of 3,3',5,5' - tetramethylbenzidine (TMB; Sigma-Aldrich) to each well.
?. The reaction is stopped with 50 µl of 3M H2SO4 solution.
?. The plate is visually assessed for the development of colour and read in a microplate reader at 450 nm.
?. A cut-off point can be calculated as the mean of the optical density of negative controls x 3. The higher the OD value the higher will be the binding affinity of SpLA to avian immunoglobulins.
TITLE: Capture and concentration of SARS-CoV-2 and other respiratory viruses from VTM / UTM samples using Magnetic Nanotrap® particles for direct RNA extraction
AUTHORS: Ben Lepene, Anurag Patnaik, Robert Barclay

[DESCRIPTION]
<div class = "text-blocks"><div class = "text-block">This protocol provides a method for Magnetic Nanotrap® particle-based capture and concentration of viruses from Viral transport media and Universal transport media samples.</div></div>

[STEPS]
?. [Virus Capture and RNA Extraction]
Add  to the sample.
[of Magnetic Nanotrap® particles]
?. [Virus Capture and RNA Extraction]
Incubate samples with Magnetic Nanotrap® particles at  for .
0 Room temperature
?. [Virus Capture and RNA Extraction]
Resuspend particle pellet in .
[of extraction buffer]
?. [Virus Capture and RNA Extraction]
Heat samples at  for .
95 °C
This step can be performed on a heat block or thermocycler.
?. [Virus Capture and RNA Extraction]
Use a magnetic rack to separate the Magnetic Nanotrap® particles from the sample.
?. [Virus Capture and RNA Extraction]
Collect the supernatant. The sample is ready for analysis.
?. [RT-PCR Reaction]
Use any SARS-CoV-2 RT-PCR detection kit. Follow manufacturer instructions to set up the RT- PCR
?. [Virus Capture and RNA Extraction]
Add  to a microcentrifuge tube.
[Viral transport media (VTM) or Universal transport media (UTM) sample]
500 µL of the sample can be used if 1 mL is not available.
?. [Virus Capture and RNA Extraction]
Add  to the pellet and resuspend to wash.
[of 1X PBS]
?. [Virus Capture and RNA Extraction]
Use a magnetic rack to separate the Magnetic Nanotrap® particles from the sample.
?. [Virus Capture and RNA Extraction]
Discard the supernatant carefully without disturbing the pellet. If required - use a smaller pipette to remove any residual PBS.
?. [Virus Capture and RNA Extraction]
Use a magnetic rack to separate the Magnetic Nanotrap® particles from the sample.
?. [Virus Capture and RNA Extraction]
Discard the supernatant carefully without disturbing the pellet.
TITLE: No Title
AUTHORS: 

[DESCRIPTION]
International and national studies on dating violence have concluded about the higher involvement of adolescents and youths in comparison to adult couples. At the same time, prevention programs have resulted more successful in adolescent population than in older ages, yielding promising results regarding the reduction of aggressive behaviour. However, despite these encouraging results, evidence-based universal prevention programs are still scarce among the scientific community, particularly in Spain. This project aims to contribute to the body of knowledge of evidence-based interventions developing, implementing and evaluating the efficacy of a Cluster-Randomized Control Trial Dating Violence Prevention Program. The program will integrate the characteristics and peculiarities of the phenomenon when it happens in the teenage years; risk and protective factors of dating violence will be considered and included in the design of the intervention program; the program will involve school member staff and will be partially sustained on peer-led learning. The results of this study will contribute to increasing the number of evaluated prevention programs to reduce dating violence in adolescence, which may be used by teachers and policymakers. By doing this, the project is therefore expected to contribute to the scientific and educational community with a psychoeducational model that could be incorporated into the educational policies.

[STEPS]