iGEM 2018 — Team Lund

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# Team:Lund/Notebook

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* [Home](/Team%3ALund)
* [People](/Team%3ALund/People)
* Project
  + [Description](/Team%3ALund/Description)
  + [Design](/Team%3ALund/Design)
  + [Product Design](/Team%3ALund/Applied_Design)
  + [Results](/Team%3ALund/Results)
  + [InterLab](/Team%3ALund/InterLab)
  + [Parts](/Team%3ALund/Parts)
  + [Protocols](/Team%3ALund/Experiments)
  + [Safety](/Team%3ALund/Safety)
  + [Notebook](/Team%3ALund/Notebook)
* [Model](/Team%3ALund/Model)
* Practices
  + [Integrated](/Team%3ALund/Human_Practices)
  + [Engagement](/Team%3ALund/Public_Engagement)

# Notebook

### Why notebooks are important

In order to follow how the work progresses and how to plan for future work, it is of great importance to clearly document the work and to reflect upon recent and ancient results to see where improvements can be made. The process of evaluation of the hard work becomes a lot easier if a structured and detailed notebook is written continuously throughout the time period of which the work is being made.

During the iGEM work-intense year 2018 a lot of different hypotheses has been tested and compared. And to keep track of all of the protocols used, a document has been written containing materials, equipments and methods.

You can find the day-by-day notebook by iGEM Lund 2018 when it comes to lab work [Here](https://static.igem.org/mediawiki/2018/4/4b/T--Lund--Notebook.pdf).

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##### Home

* [Home](/Team%3ALund)

##### Team

* [Team](/Team%3ALund/Team)
* [Collaborations](/Team%3ALund/Collaborations)
* [Attributions](/Team%3ALund/Attributions)

##### Project

* [Description](/Team%3ALund/Description)
* [Design](/Team%3ALund/Design)
* [Results](/Team%3ALund/Results)
* [InterLab](/Team%3ALund/InterLab)
* [Parts](/Team%3ALund/Parts)
* [Protocol](/Team%3ALund/Experiments)
* [Safety](/Team%3ALund/Safety)
* [Notebook](/Team%3ALund/Notebook)

##### Model

* [Landing page](/Team%3ALund/Model)
* [Hemoglobin](/Team%3ALund/Model/Hemoglobin)
* [Growth curves](/Team%3ALund/Model/GrowthCurves)

##### Practices

* [Integrated](/Team%3ALund/Human_Practices)* [Engagement](/Team%3ALund/Public_Engagement)

#### Contact

Sölvegatan 22 A
223 62 Lund
Sweden
igemlund@gmail.com

#### Stay connected

* [Facebook](https://www.facebook.com/igemlund)
* [Instagram](https://www.instagram.com/igem_lund)
* [Twitter](https://twitter.com/iGEM_Lund)

©2018 iGEM Lund


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# Team:Lund/Experiments

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* [Home](/Team%3ALund)
* [People](/Team%3ALund/People)
* Project
  + [Description](/Team%3ALund/Description)
  + [Design](/Team%3ALund/Design)
  + [Product Design](/Team%3ALund/Applied_Design)
  + [Results](/Team%3ALund/Results)
  + [InterLab](/Team%3ALund/InterLab)
  + [Parts](/Team%3ALund/Parts)
  + [Protocols](/Team%3ALund/Experiments)
  + [Safety](/Team%3ALund/Safety)
  + [Notebook](/Team%3ALund/Notebook)
* [Model](/Team%3ALund/Model)
* Practices
  + [Integrated](/Team%3ALund/Human_Practices)
  + [Engagement](/Team%3ALund/Public_Engagement)

# Protocols

### The importance of protocols

Science is an old and everlasting opportunity to learn more about nature, life and its components. In order to do this in a good way, knowledge is a prerequisite. The combined knowledge is always higher than for the individual. This means that the higher knowledge is reached if collaboration, comparison and competitiveness is present. Another very important factor is to replicate and compare results from one laboratory to another to verify and strengthen results an conclusions. In order to be able to make good comparisons ,exactly similar protocols will have to be used. Therefore a good documentation of what was used, in what quantities and how these are used, are of high importance.

During the iGEM work-intense year 2018 a lot of different hypotheses has been tested and compared. And to keep track of all of the protocols used, a document has been written containing materials, equipments and methods.

You can find the protocols used by iGEM Lund 2018 [Here](http://2018.igem.org/wiki/images/2/2b/T--Lund--protocols.pdf).

![Gant Logo](http://2018.igem.org/wiki/images/7/76/T--Lund--sponsor_gant.png)
![LMK Logo](http://2018.igem.org/wiki/images/7/7c/T--Lund--sponsor_lmk.png)
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![Kunskappsartner Logo](http://2018.igem.org/wiki/images/1/14/T--Lund--sponsor_kunskap_partner.png)

##### Home

* [Home](/Team%3ALund)

##### Team

* [Team](/Team%3ALund/Team)
* [Collaborations](/Team%3ALund/Collaborations)
* [Attributions](/Team%3ALund/Attributions)

##### Project

* [Description](/Team%3ALund/Description)
* [Design](/Team%3ALund/Design)
* [Results](/Team%3ALund/Results)
* [InterLab](/Team%3ALund/InterLab)
* [Parts](/Team%3ALund/Parts)
* [Protocol](/Team%3ALund/Experiments)
* [Safety](/Team%3ALund/Safety)
* [Notebook](/Team%3ALund/Notebook)

##### Model

* [Landing page](/Team%3ALund/Model)
* [Hemoglobin](/Team%3ALund/Model/Hemoglobin)
* [Growth curves](/Team%3ALund/Model/GrowthCurves)

##### Practices

* [Integrated](/Team%3ALund/Human_Practices)* [Engagement](/Team%3ALund/Public_Engagement)

#### Contact

Sölvegatan 22 A
223 62 Lund
Sweden
igemlund@gmail.com

#### Stay connected

* [Facebook](https://www.facebook.com/igemlund)
* [Instagram](https://www.instagram.com/igem_lund)
* [Twitter](https://twitter.com/iGEM_Lund)

©2018 iGEM Lund

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# Team:Lund/Results

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[![iGEM Lund](http://2018.igem.org/wiki/images/b/b6/T--Lund--logo_rescaled.svg)](/Team%3ALund)

* [Home](/Team%3ALund)
* [People](/Team%3ALund/People)
* Project
  + [Description](/Team%3ALund/Description)
  + [Design](/Team%3ALund/Design)
  + [Product Design](/Team%3ALund/Applied_Design)
  + [Results](/Team%3ALund/Results)
  + [InterLab](/Team%3ALund/InterLab)
  + [Parts](/Team%3ALund/Parts)
  + [Protocols](/Team%3ALund/Experiments)
  + [Safety](/Team%3ALund/Safety)
  + [Notebook](/Team%3ALund/Notebook)
* [Model](/Team%3ALund/Model)
* Practices
  + [Integrated](/Team%3ALund/Human_Practices)
  + [Engagement](/Team%3ALund/Public_Engagement)

# Results

* [Overview](/Team%3ALund/Results)
* [BioBricks](/Team%3ALund/Results/BioBricks)
* [Growth curves](/Team%3ALund/Results/GrowthCurves)
* [GFP production](/Team%3ALund/Results/GFPProduction)
* [Fermentor](/Team%3ALund/Results/Fermentor)
* [Human hemoglobin](/Team%3ALund/Results/HumanHemoglobin)

### Overview

In order to get good results, frequent checkpoints has to be used. It is important checking that the biobricks are correctly assembled and that they in fact contain the desired DNA. This is shown in the result section in the “BioBrick” tab. Then it’s important to see how the inserts affects the growth rate, shown and analysed in the tab for “Growth curves”. When the growth effect is determined the real results can be obtained. These are how the GFP production is affected with different VHb concentrations, shown in the tab for “VHB & GFP production”. It is also interesting to see what happens if the systems of VHb+GFP is different on a larger scale. How the measurements went when using a bioreactor is shown in the tab for “Fermentor”.

As a side-project, connected with the human hemoglobin model, mutants of different adult human hemoglobins were evaluated which you can read more about it in the tab for “Human hemoglobin”.

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![LMK Logo](http://2018.igem.org/wiki/images/7/7c/T--Lund--sponsor_lmk.png)
![Lund Logo](http://2018.igem.org/wiki/images/8/8a/T--Lund--sponsor_lund.png)
![Kunskappsartner Logo](http://2018.igem.org/wiki/images/1/14/T--Lund--sponsor_kunskap_partner.png)

##### Home

* [Home](/Team%3ALund)

##### Team

* [Team](/Team%3ALund/Team)
* [Collaborations](/Team%3ALund/Collaborations)
* [Attributions](/Team%3ALund/Attributions)

##### Project

* [Description](/Team%3ALund/Description)
* [Design](/Team%3ALund/Design)
* [Results](/Team%3ALund/Results)
* [InterLab](/Team%3ALund/InterLab)
* [Parts](/Team%3ALund/Parts)
* [Protocol](/Team%3ALund/Experiments)
* [Safety](/Team%3ALund/Safety)
* [Notebook](/Team%3ALund/Notebook)

##### Model

* [Landing page](/Team%3ALund/Model)
* [Hemoglobin](/Team%3ALund/Model/Hemoglobin)
* [Growth curves](/Team%3ALund/Model/GrowthCurves)

##### Practices

* [Integrated](/Team%3ALund/Human_Practices)* [Engagement](/Team%3ALund/Public_Engagement)

#### Contact

Sölvegatan 22 A
223 62 Lund
Sweden
igemlund@gmail.com

#### Stay connected

* [Facebook](https://www.facebook.com/igemlund)
* [Instagram](https://www.instagram.com/igem_lund)
* [Twitter](https://twitter.com/iGEM_Lund)

©2018 iGEM Lund