iGEM 2016 — Team HSiTAIWAN

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# Team:HSiTAIWAN/Notebook

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X

Oh hello there,I am Charlie,a trusty member of the "Herb Tasters" and also the brainiest E. coli in the colony.

　I know all the secrets of Chinese herbs and their magical healing powers.

　If you are up to a challenge,find me at team HSiTW at the jamboree.

　I am the one in a straw hat,showing them pearls.I will be waiting.

　Muhahahahaha

Hi there! My name is Nu Zhen Chi. This is how I look like.

　　Take a closer look; guess which part of me is used as medicine?

(1) the root ![](https://static.igem.org/mediawiki/2016/e/e2/T--HSiTAIWAN--Drherb_Q1.png)

(2) the stem

(3) the leaf

(4) the seed

**Ans.**(4) the seed

　**Name:** 女貞子 (Nu Zhen Chi)

　**Botanical Name:** Ligustrum lucidum Aiton
![](https://static.igem.org/mediawiki/2016/8/8d/T--HSiTAIWAN--Drherb_A1.png)

　I can treat people who are yin deficient, and liver problems that cause dizziness,cataract of the eyes,

lower back pain, premature graying of the hair and tinnitus.

Hello! My name is Chuan Xiong. This is how I look like.

　Make a guess, which part of me is used as medicine?

(1) the root ![](https://static.igem.org/mediawiki/2016/2/2d/T--HSiTAIWAN--Drherb_Q2.png)

(2) the stem

(3) the leaf

(4) the seed

**Ans.**(1) the root

　**Name:** 川芎 (Chuan Xiong)

　**Botanical Name:** Ligusticum chuanxiong Hort
![](https://static.igem.org/mediawiki/2016/8/8d/T--HSiTAIWAN--Drherb_A2.png)

　I help with blood regulation to prevent relevant to blood stasis and non-stop bleeding.I can also strengthen your qi circulation.

　In addition, I relieve you of physical pain,
such as headaches, abdominal aches, chest pain, and muscle pain.

　Finally, I free the ladies of menstrual disorders and amenorrhea.

What’s up? My name is Dang Gui. I can:

(1) stop coughing ![](https://static.igem.org/mediawiki/2016/5/5e/T--HSiTAIWAN--Drherb_Q3.png)

(2) regulate mense

(3) reduce internal heat

**Ans.**(2) Regulate mense

　**Name:** 當歸 (Dang Gui)

　**Botanical Name:** Angelica sinensis (Oliv.) Diels
![](https://static.igem.org/mediawiki/2016/1/12/T--HSiTAIWAN--Drherb_A3.png)

　I can remove blood stasis and clots, so I am usually used to regulate menses,lubricate intestines to correct constipation, reduce swelling, expel pus.

**Reference**

　臧堃堂 (2005) 中華材輕百科-現代版本草綱目，山岳文化出版社，台北

　[Non-Profit Organization Brion Research Institute of Taiwan.](http://brion.org.tw/index.php)

　[Chinese Herb Gallery. Jade Institute](https://www.jadeinstitute.com/index.php)

　[Herbal Glossary. Shen-Nong- Chinese Traditional Medicine](http://www.shen-nong.com/eng/front/index.html)

　**Acknowledgement**

　Thank you for Non-Profit Organization Brion Resaerch Institute of Taiwan that provide us Chinese herbs and photos.

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- Functional Assay

  * Pb
  * Cu
  * As
  * CYP
- Chinese Medicine testing

  * Pb
  * Cu
  * As
  * CYP

Notebook

Framework

There main target in our experiment for us to detect is Chinese herbal medicine. We first did some pretest to know that the toxins won’t kill our E.coli. Second, we test the function of our plasmids, then assay the function in Chinese medicines. Last we add in more than one kind of toxins to ensure other toxins won’t interfere the reaction in our E. coli.

Functional Assay

[Pb](https://2016.igem.org/Team%3AHSiTAIWAN/Notebook/FA/Pb)

[Cu](https://2016.igem.org/Team%3AHSiTAIWAN/Notebook/FA/Cu)

[As](https://2016.igem.org/Team%3AHSiTAIWAN/Notebook/FA/As)

[CYP](https://2016.igem.org/Team%3AHSiTAIWAN/Notebook/FA/CYP)

Chinese Medicine testing

[Pb](https://2016.igem.org/Team%3AHSiTAIWAN/Notebook/CM/Pb)

[Cu](https://2016.igem.org/Team%3AHSiTAIWAN/Notebook/CM/Cu)

[As](https://2016.igem.org/Team%3AHSiTAIWAN/Notebook/CM/As)

[CYP](https://2016.igem.org/Team%3AHSiTAIWAN/Notebook/CM/CYP)

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