iGEM 2019 — Team KAIT_JAPAN

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# Team:KAIT JAPAN/Notebook

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[KAIT JAPAN](https://2019.igem.org/Team%3AKAIT_JAPAN)

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# Menu

* [Top](https://2019.igem.org/Team%3AKAIT_JAPAN)
* Project
  + [Background](https://2019.igem.org/Team%3AKAIT_JAPAN/background)
  + [Design](https://2019.igem.org/Team%3AKAIT_JAPAN/design)
  + [Experimental Method](https://2019.igem.org/Team%3AKAIT_JAPAN/methods)
  + [iGEM Lab Note](https://2019.igem.org/Team%3AKAIT_JAPAN/lab_note)
  + [Safety](https://2019.igem.org/Team%3AKAIT_JAPAN/safety)
  + [Results](https://2019.igem.org/Team%3AKAIT_JAPAN/results)
  + [Achievements](https://2019.igem.org/Team%3AKAIT_JAPAN/achievements)
  + [Human Practice](https://2019.igem.org/Team%3AKAIT_JAPAN/human_practice)
* [Members](https://2019.igem.org/Team%3AKAIT_JAPAN/members)

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)


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# Team:KAIT JAPAN/Experiments

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[KAIT JAPAN](https://2019.igem.org/Team%3AKAIT_JAPAN)

[Menu](#offcanvas-slide)

# Menu

* [Top](https://2019.igem.org/Team%3AKAIT_JAPAN)
* Project
  + [Background](https://2019.igem.org/Team%3AKAIT_JAPAN/background)
  + [Design](https://2019.igem.org/Team%3AKAIT_JAPAN/design)
  + [Experimental Method](https://2019.igem.org/Team%3AKAIT_JAPAN/methods)
  + [iGEM Lab Note](https://2019.igem.org/Team%3AKAIT_JAPAN/lab_note)
  + [Safety](https://2019.igem.org/Team%3AKAIT_JAPAN/safety)
  + [Results](https://2019.igem.org/Team%3AKAIT_JAPAN/results)
  + [Achievements](https://2019.igem.org/Team%3AKAIT_JAPAN/achievements)
  + [Human Practice](https://2019.igem.org/Team%3AKAIT_JAPAN/human_practice)
* [Members](https://2019.igem.org/Team%3AKAIT_JAPAN/members)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

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# Team:KAIT JAPAN/results

[![](https://2019.igem.org/wiki/images/c/cc/T--KAIT_JAPAN--logo_1.png)](https://2019.igem.org/Team%3AKAIT_JAPAN)

[KAIT JAPAN](https://2019.igem.org/Team%3AKAIT_JAPAN)

[Menu](#offcanvas-slide)

# Menu

* [Top](https://2019.igem.org/Team%3AKAIT_JAPAN)
* Project
  + [Background](https://2019.igem.org/Team%3AKAIT_JAPAN/background)
  + [Design](https://2019.igem.org/Team%3AKAIT_JAPAN/design)
  + [Experimental Method](https://2019.igem.org/Team%3AKAIT_JAPAN/methods)
  + [iGEM Lab Note](https://2019.igem.org/Team%3AKAIT_JAPAN/lab_note)
  + [Safety](https://2019.igem.org/Team%3AKAIT_JAPAN/safety)
  + [Results](https://2019.igem.org/Team%3AKAIT_JAPAN/results)
  + [Achievements](https://2019.igem.org/Team%3AKAIT_JAPAN/achievements)
  + [Human Practice](https://2019.igem.org/Team%3AKAIT_JAPAN/human_practice)
* [Members](https://2019.igem.org/Team%3AKAIT_JAPAN/members)

# Results

Fig. 1 and 2 shows the results of colony PCR of
*E. coli* transformed to confirm whether the target DNAs genes
① and ② synthesized have been cloned into the vector BBa\_J04450.

![](https://2019.igem.org/wiki/images/d/d3/T--KAIT_JAPAN--results_1.png)

Fig. 1 Genes ①

![](https://2019.igem.org/wiki/images/4/43/T--KAIT_JAPAN--results_2.png)

Fig. 2 Genes ②

For Fig. 1, bands were confirmed in lanes 1, 2, 3, and 4 nearby the
target molecular weight of 720 bp.
For Fig. 2, bands were confirmed in lanes 1, 2, 3, and 4 nearby the
target molecular weight of 1400 bp.

Furthermore, sequencing was performed to confirm that the cloning
was performed correctly. And we get results that genes ① was 100%
identical to the target sequence, but we failed to identify our
target sequence in genes ②.

A vector containing the promoter of the target gene ① (BBa\_I0500,
BBa\_14032, BBa\_I13453) fig. 3, 4 and 5 in order shows the results of
colony PCR of transformed E. coli to confirm that they were
successfully cloned.

![](https://2019.igem.org/wiki/images/4/4a/T--KAIT_JAPAN--results_3.png)

Fig. 3 BBa\_I0500

![](https://2019.igem.org/wiki/images/d/d5/T--KAIT_JAPAN--results_4.png)

Fig. 4 BBa\_I13453

![](https://2019.igem.org/wiki/images/5/55/T--KAIT_JAPAN--results_5.png)

Fig. 5 BBa\_I14032

Fig. 3 shows no band in all lanes 2, 3, 4 and 5.
In Fig. 4, bands were confirmed in lanes 3, 4, and 5 nearby the
target molecular weight of 810 bp.
In Fig. 5, a band near to the target molecular weight of 765 bp was
confirmed in lanes 2, 3, and 4.
From the results shown above, we know that the plasmid we designed
was constructed succesfully.