iGEM 2015 — Team Tec-Monterrey

# Team:Tec-Monterrey/Notebook

iGEM MTY 2015

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[![](https://static.igem.org/mediawiki/2015/d/d1/Tec-Monterrey_SimpleLogo.png)](https://2015.igem.org/Team%3ATec-Monterrey)

* [Main](https://2015.igem.org/Team%3ATec-Monterrey)
* [Research](https://2015.igem.org/Team%3ATec-Monterrey/Description)
* [Notebook](https://2015.igem.org/Team%3ATec-Monterrey/Notebook)
* [Modeling](https://2015.igem.org/Team%3ATec-Monterrey/Modeling)
* [Outreach](https://2015.igem.org/Team%3ATec-Monterrey/Outreach)
* [Collaborations](https://2015.igem.org/Team%3ATec-Monterrey/Collaborations)
* [Policies and Practices](https://2015.igem.org/Team%3ATec-Monterrey/Practices)
* [Safety](https://2015.igem.org/Team%3ATec-Monterrey/Safety)
* [Achievements](https://2015.igem.org/Team%3ATec-Monterrey/Achievements)
* [Team](https://2015.igem.org/Team%3ATec-Monterrey/Attributions)

![](https://static.igem.org/mediawiki/2015/5/53/Tec-Monterrey_main-e7c542.jpg)

June
July
August
September

| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
| --- | --- | --- | --- | --- | --- | --- |
|  | 1 | 2 | 3 | 4 | 5 | 6 |
| 7 | 8 | 9 | 10 | 11 | 12 | 13 |
| 14 | 15 | 16 | 17 | 18 | 19 | 20 |
| 21 | 22  Miniprep (Dania, Hiyam) Digestion (Carolina) Electrophoresis (David, Alex) | 23  Stock RFP (Dania) | 24 | 25  Miniprep (Hiyam, Dania) Digestion (Ausencio, Dania, Hiyam) Electrophoresis (Ausencio, Dania, Hiyam) | 26  Miniprep (Ausencio, Dania, Hiyam) Digestion (Ausencio, Dania, Hiyam) Electrophoresis (Karen Carrizales, Dania) | 27  Calcium-competent cells (Jesús) |
| 28  Transformation (David, Ernesto) | 29  Inoculations (Alejandro, Carolina, Jesús, Dania) | 30  Miniprep (David, Alejandro) Digestion (Alejandro, Hiyam) Electrophoresis (Alejandro, Ernesto) Digestion (Alejandro, David, Ernesto) Electrophoresis (David, Ernesto) |  |  |  |  |

| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
| --- | --- | --- | --- | --- | --- | --- |
|  |  |  | 1  Purification (David, Minerva, Ernesto) Digestions (Carolina, Karen Carrizales) Electrophoresis (Carolina, Dania, Ausencio) Ligations (Jesús, Alex, Hiyam) | 2  Purification (Carolina, Alejandro) Ligations (Carolina, Alejandro) Transformation (David, Karen Carrizales) | 3  Stock P4 (Hiyam) Miniprep (Hiyam, Carolina) | 4  Digestion (Hiyam, David) Electrophoresis (Carolina) Transformation (David, Jesús) Digestion (Karen Carrizales, Alejandro) Electrophoresis (Alejandro, Karen Carrizales, David) Purification (Hiyam, Dania) Ligations (Hiyam, Alejandro, Dania) |
| 5  Transformation (Jesús, Carolina) Inoculations (David, Dania, Karen Carrizales, Alejandro) | 6  Stocks P1, P2, P3, GFP (Carolina, Dania) Miniprep (Carolina, Dania, Ausencio) Inoculations (Jesus, Hiyam) | 7  Degestions (Carolina, Dania, Hiyam) Miniprep (Hiyam, Jesús, David, Ausencio) Digestions (Ausencio, Dania, Hiyam) | 8  Electrophoresis (David, Hiyam, Karen Carrizales, Dania) Purification (Karen Carrizales, Ausencio, David) Electrophoresis (Hiyam, Dania) Ligations (David, Karen Carrizales, Ernesto) Transformation (Minerva) | 9  Electrophoresis (David, Ausencio, Ernesto) Digestion (David, Ernesto) Electrophoresis (David, Carolina, Dania) Purification (Hiyam, Dania) Ligations (David) Miniprep Lowe Budget (Alejandra, Karen Tello) | 10  Digestion (Carolina) Electrophoresis (David, Ausencio) Transformation (David, Ausencio) Purification (Ausencio, David, Dania, Karen Carrizales) Digestion Low Budget (Alejandra, Karen Tello) | 11  Ligations (Carolina) |
| 12  Transformation (Carolina, Jesús, Ausencio) | 13  Inoculations (Carolina, David, Karen Carrizales) | 14  Miniprep (Carolina, Ausencio, David) Digestion (Hiyam, David) Transformation (Karen Carrizales, David) Electrophoresis (Hiyam, David) | 15  Inoculations (Karen Carrizales, Jesús) | 16  Miniprep (David, Hiyam) Digestion (David, Hiyam) Electrophoresis (Carolina, Karen Carrizales) Purification (Hiyam, Karen Carrizales, David) | 17  Antibiotic preparation (Hiyam, Carolina) Transformation (Karen Carrizales, David) | 18 |
| 19  Miniprep (David) Digestion (Karen Carrizales, Ausencio) Electrophoresis, Purification (Hiyam, David) Inoculations (Ausencio) | 20  Miniprep (David, Hiyam) Miniprep Low Budget, Digestion Lowe Budget (Alejandra, Karen Tello) | 21  Digestion (Hiyam, Alejandra, Claudia) Electrophoresis (Karen Carrizales, Hiyam) Miniprep (David) Digestion (Hiyam, David) Electrophoresis (Hiyam) | 22  Calcium-competent cells (Claudia) | 23 | 24  Miniprep (Hiyam, Dania, Carolina) Calcium-competent cells (Claudia) Transformation, Stocks P1 P2 P3 TOP10 DH5alpha (Alejandro) Digestion (Hiyam, Caro, Dania) Electrophoresis (David, Karen Carrizales) | 25 |
| 26 | 27  Miniprep (Dania, Hiyam, Caro) Digestion, Electrophoresis (David, Alejandro) Digestion (Carolina, Alejandro) Sf9 cells counting and passing (Minerva) | 28 | 29  Digestion (Carolina, David, Hiyam) Electrophoresis (Hiyam, Alejandro) Transformation (Alejandro) Sf9 cells counting and passing (Dania, Claudia) | 30  Digestion (Hiyam, Dania, David) Electrophoresis (David, Hiyam, Carolina) Purification (Alejandro, Hiyam) Digestion (Alejandro, Hiyam) Electrophoresis (Hiyam, Alejandro, David) | 31  Electrophoresis (Alejandro, David) Purification (Carolina, David) Digestion (Carolina, Alejandro, Hiyam, Dania) Electrophoresis (David) |  |

| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
| --- | --- | --- | --- | --- | --- | --- |
|  |  |  |  |  |  | 1  Purification (Hiyam, Dania) Ligation (Hiyam, Alex, Dania) Inoculations (Dania, Hiyam, Alex, David) Transformation (Carolina, Hiyam, Alejandro) |
| 2  Digestion, Electrophoresis (Alejandro) Calcium-competent cells (Minerva, David) Transformation (Alejandro, Hiyam) Sf9 cells counting and passing (Dania, Claudia) | 3  Stocks C2, C4, C5, P4, pFastBac (Alejandro, Hiyam) Miniprep (David, Hiyam) Digestion (Hiyam, David, Dania) Calcium-competent cells (Jesús, Karen Carrizales), Electrophoresis (Carolina, Dania) | 4  Digestion, Electrophoresis (Alejandro, Karen Carrizales, David) | 5  Cell Lysis (Alejandro) Transformation (Hiyam, Alejandro, Karen Tello) | 6  Ligation, Digestion (Hiyam, Alejandro) Miniprep (Hiyam) | 7  Miniprep 1 (David) Miniprep 2 (Alejandro, Karen Tello) Digestion (Karen Tello, Lourdes, Jesús) Electrophoresis (Karen Tello, Lourdes) Sf9 cells counting, passing and criopreservation (Dania, Claudia) | 8 |
| 9 | 10  Electrophoresis (Alejandro) Inoculations (Jesús, David) | 11  Miniprep 1 (Carolina, Karen Carrizales) Miniprep 2 (David) Miniprep 3 (Karen Tello, Alejandro, Hiyam) Digestion (Hiyam) Bacmid extraction (David) Sf9 cells counting and passing (Dania, Claudia) | 12  Electrophoresis, Miniprep (Hiyam) Electrophoresis (Karen Tello, Ausencio) Inoculations (Carolina) Transfection (David, Dania, Minerva) | 13  Miniprep (Carolina) Transformation (Alejandro) | 14  SDS-Page (David) Western Blot (Minerva, David) | 15  Luminiscence readings, Biuret Assay (Alejandro) Sf9 cells counting and passing (Dania, Claudia) |
| 16  Sf9 cells counting and passing (Dania, Claudia) SDS-Page (David) Western Blot (David, Minerva) Luminiscence readings (Minerva, Alejandro) Virus amplification (Jesús, Karen Carrizales, Claudia) | 17 | 18  Calcium-competents (Alejandro, Hiyam, Ausencio) Transformation, Digestion, Electrophoresis (Hiyam, Karen Tello, Ausencio) Purification, Ligation (Hiyam, Carolina) Inoculations, Miniprep (Carolina, Karen Tello) | 19 | 20  Sf9 cells counting and passing (Dania, Claudia) | 21 | 22  Sf9 cells counting and passing (Dania, Claudia) |
| 23  Minprep (David) Digestion (Carolina) Bacmid extraction (Karen Carrizales, Carolina) Electrophoresis (Carolina) Inoculations (Karen Tello, Carolina) Sf9 cells counting and passing (Dania, Claudia) | 24  Transfection (David, Dania, Minerva) | 25  Miniprep, Digestion, Electrophoresis, Gibson Assembly (Hiyam, Karen Tello, Ausencio) Bradford (Alejandro) Sf9 cells counting and passing (Dania, Claudia) | 26 | 27  Digestion (Alejandro) | 28  Miniprep, Digestion, Electrophoresis (Hiyam, Alejandro, Ausencio) Purification (Eduardo, Hiyam, Alejandro, Ausencio) | 29  Gibson Assembly (Eduardo, Alejandro, Hiyam, Ausencio) Sf9 cells counting and passing (Dania, Claudia) |
| 30  Viral plaque assay (Jesús, Karen Carrizales, Claudia) | 31  Sf9 cells counting and passing (Dania, Claudia) |  |  |  |  |  |

| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
| --- | --- | --- | --- | --- | --- | --- |
|  |  | 1  Sf9 cells counting and passing (Dania, Claudia) | 2 | 3 | 4  Sf9 cells counting and passing (Dania, Claudia) | 5  Digestion (Hiyam) Sf9 cells counting and passing (Dania, Claudia) |
| 6  Sf9 cells counting and passing (Dania, Claudia) Viral plaque assay (Jesús, Karen Carrizales, Claudia) | 7  Gibson Assembly (Hiyam) Sf9 cells counting and passing (Dania, Claudia) | 8  Sf9 cells counting and passing (Dania, Claudia) | 9  Gibson Assembly (Eduardo, Hiyam, Alejandro, Ausencio) Sf9 cells counting and passing (Dania, Claudia) | 10  Sf9 cells counting and passing (Dania, Claudia) Transfection (David, Dania, Minerva) | 11  Sf9 cells counting and passing (Dania, Claudia) | 12  Sf9 cells counting and passing (Dania, Claudia |
| 13  Viral plaque staining, viral plaque assay (Karen Carrizales, Jesús) | 14 | 15  Digestion (Alejandro, Ausencio) Electrophoresis (David, Eduardo, Ausencio) IMAC chromatography (Karen Carrizales, Jesús, Alejandro) SDS-Page (David) Western Blot (David, Minerva) | 16  Miniprep (Alejandro, Hiyam) SDS-Page, Western Blot (David, Minerva) | 17  Confirmation of parts for submitting (Karen Tello, Ausencio, Hiyam, Alejandro, Eduardo) | 18 | 19 |
| 20 | 21 | 22 | 23 | 24 | 25 | 26 |
| 27 | 28 | 29 | 30 | 31 |  |  |