iGEM 2012 — Team UANL_Mty-Mexico

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# Team:UANL Mty-Mexico/Notebook

### From 2012.igem.org

iGEM UANL 2012

* [Home](https://2012.igem.org/Team%3AUANL_Mty-Mexico)
* [Team](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Team)
  + [Members](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Team)
  + [Profile](https://igem.org/Team.cgi?year=2012&team_name=UANL_Mty-Mexico)
* [Project](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Project/abstract)
  + [Abstract](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Project/abstract)
  + [Overview](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Project)
  + [Detection](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Project/detection)
  + [Capture](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Project/capture)
  + [Recovery](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Project/recovery)
  + [Circuit](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Project/circuit)
* [Modeling](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Modeling)
  + [Overview](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Modeling)
  + [Transport](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Modeling/transport_and_accumulation)
  + [Biosensor](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Modeling/Biosensor)
  + [Silica Binding](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Modeling/Silica_binding)
  + [Molecular Modeling](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Modeling/3Dmodel)
* [Outreach](https://2012.igem.org/Team%3AUANL_Mty-Mexico/hp)
  + [Human Practices](https://2012.igem.org/Team%3AUANL_Mty-Mexico/hp)
  + [SynBio Tunnel](https://2012.igem.org/Team%3AUANL_Mty-Mexico/hp/tunnel)
* [Notebook](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Notebook)
  + [Cloning strategy](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Notebook)
  + [Wetlab](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Notebook/wetlab)
  + [Protocols](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Notebook/protocols)
* [Parts](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Parts)
* [Safety](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Safety)
  + [Overview](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Safety)
* [Attributions](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Attributions)

### Cloning Strategy

**Fusion proteins**

We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.

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| ![](https://static.igem.org/mediawiki/2012/thumb/a/a5/Q_Sites.png/800px-Q_Sites.png) |

**Figure 1.** Cloning strategy for contructions of fusion proteins.

| ![](https://static.igem.org/mediawiki/2012/thumb/5/5f/Assembly_21-2.png/569px-Assembly_21-2.png) |

For the rest of the constructs Standard Assembly 10 was used. Check our results on the "wet lab" tab.

[* **>>Cloning Strategy**](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Notebook)
[* Wetlab](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Notebook/wetlab)
[* Protocols](https://2012.igem.org/Team%3AUANL_Mty-Mexico/Notebook/protocols)

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