iGEM 2008 — Team Beijing_Normal

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# Team:Beijing Normal/Notebook

### From 2008.igem.org

| [Home](/Team%3ABeijing_Normal "Team:Beijing Normal") | [The Team](/Team%3ABeijing_Normal/Team "Team:Beijing Normal/Team") | [The Project](/Team%3ABeijing_Normal/Project "Team:Beijing Normal/Project") | [Parts Submitted to the Registry](/Team%3ABeijing_Normal/Parts "Team:Beijing Normal/Parts") | **Notebook** |
| --- | --- | --- | --- | --- |

## **Dynamics**

* == **Communications** ==
  + 17th June: Attend the Asian Workshop meeting.

* + 17th July: Meet up with Tsinghua Team, and learn fron their experiences.

* + 25th July: With Invitrogen.Co. Ltd and Tsinghua team to sign up a contract.

* + 27th July: Have a discussion with Tsinghua team about the transformation efficiency.

We generalize some key words from the discussion:

1. Make sure the concentration of the plasmids from the biobrick is enough for transformation.

2. Keep a low temprature when enzyme digestion is performed

3. The transformation product are always incubated for more than 1h.

* + 9th September: We and Tsinghua team get together in our lab. We have a happy time together and share a lot.

* + 10th September: We provide Tsinghua Team with the cells harboring pSB1AC3 plasmids. We did the the tramsformation for them. Because they found their transformation effeciency is relatively low.

* + 17th September: Tsinghua Team told us that the vector that we provided proved to work well. We were all pretty happy about this positive result.

* + 3th October: We and Chiba Team had a happy conversation via googletalk today. We share all the success and frustrations in the process. And we exchange our opinion about promotor function measurement. They told us that they have used BBa\_T9002 because their project uses quorum sensing and the part(T9002) was GFP producer controlled by 3OC6HSL Receiver Device.And their result is pretty satisfying.

* + 5th October: We share the experience of measurment with Tokyo Tech University Team. They tell us to use BBa\_I13522 (http://partsregistry.org/Part:BBa\_I13522) as a positive control when we measure the function of promotors.

* + 9th October: We test the founction of Part BBa\_I719015. The result indicates that it works well. The cells (BL21(DE3) as host) turn green when induced by IPTG.

* + 15th October: Our T-shirt is designed today. It is very pretty. See image below.

* + 19th, 20th October: We have a good discussion with USTC team in Hefei and they give us several important suggestions on experiments.

[![T-shirt.jpg](/wiki/images/0/09/T-shirt.jpg)](/File%3AT-shirt.jpg)

[![T-shirt1.jpg](/wiki/images/0/0c/T-shirt1.jpg)](/File%3AT-shirt1.jpg)

**Our logo is designed finally. It is a visual field through a microscope. You could see us in this microscope.**

[![Our logo.jpg](/wiki/images/d/d8/Our_logo.jpg)](/File%3AOur_logo.jpg)

* + Our iGem is coming to the end. But our projet will continue.

Of course, we encountered many obstacles and difficulties such as financial problems, cooperation and communication problems of teammates, spirit pressure, time management and so on. But all the hardship turned out to be a precious experience that helped us grow up. Anyway, iGem is one of our most precious experiences and we will never forget these days and night in the lab and meeting room, all the laughs and tears. We have learned a lot in this project except the attending the competition itself.

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| July | | | | | | |
| M | T | W | T | F | S | S |
|  | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/1\_July\_2008&action=edit 1] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/2\_July\_2008&action=edit 2] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/3\_July\_2008&action=edit 3] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/4\_July\_2008&action=edit 4] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/5\_July\_2008&action=edit 5] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/6\_July\_2008&action=edit 6] |
| [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/7\_July\_2008&action=edit 7] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/8\_July\_2008&action=edit 8] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/9\_July\_2008&action=edit 9] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/10\_July\_2008&action=edit 10] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/11\_July\_2008&action=edit 11] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/12\_July\_2008&action=edit 12] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/13\_July\_2008&action=edit 13] |
| [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/14\_July\_2008&action=edit 14] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/15\_July\_2008&action=edit 15] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/16\_July\_2008&action=edit 16] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/17\_July\_2008&action=edit 17] | [http://2008.igem.org/Beijing\_Normal/18\_July\_2008 18] | [http://2008.igem.org/Beijing\_Normal/19\_July\_2008 19] | [http://2008.igem.org/Beijing\_Normal/20\_July\_2008 20] |
| [http://2008.igem.org/Beijing\_Normal/21\_July\_2008 21] | [http://2008.igem.org/Beijing\_Normal/22\_July\_2008 22] | [http://2008.igem.org/Beijing\_Normal/23\_July\_2008 23] | [http://2008.igem.org/Beijing\_Normal/24\_July\_2008 24] | [http://2008.igem.org/Beijing\_Normal/25\_July\_2008 25] | [http://2008.igem.org/Beijing\_Normal/26\_July\_2008 26] | [http://2008.igem.org/Beijing\_Normal/27\_July\_2008 27] |
| [http://2008.igem.org/Beijing\_Normal/28\_July\_2008 28] | [http://2008.igem.org/Beijing\_Normal/29\_July\_2008 29] | [http://2008.igem.org/Beijing\_Normal/30\_July\_2008 30] | [http://2008.igem.org/Beijing\_Normal/31\_July\_2008 31] |

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| August | | | | | | |
| M | T | W | T | F | S | S |
|  |  |  |  | [http://2008.igem.org/Beijing\_Normal/1\_August\_2008 1] | [http://2008.igem.org/Beijing\_Normal/2\_August\_2008 2] | [http://2008.igem.org/Beijing\_Normal/3\_August\_2008 3] |
| [http://2008.igem.org/Beijing\_Normal/4\_August\_2008 4] | [http://2008.igem.org/Beijing\_Normal/5\_August\_2008 5] | [http://2008.igem.org/Beijing\_Normal/6\_August\_2008 6] | [http://2008.igem.org/Beijing\_Normal/7\_August\_2008 7] | [http://2008.igem.org/Beijing\_Normal/8\_August\_2008 8] | [http://2008.igem.org/Beijing\_Normal/9\_August\_2008 9] | [http://2008.igem.org/Beijing\_Normal/10\_August\_2008 10] |
| [http://2008.igem.org/Beijing\_Normal/11\_August\_2008 11] | [http://2008.igem.org/Beijing\_Normal/12\_August\_2008 12] | [http://2008.igem.org/Beijing\_Normal/13\_August\_2008 13] | [http://2008.igem.org/Beijing\_Normal/14\_August\_2008 14] | [http://2008.igem.org/Beijing\_Normal/15\_August\_2008 15] | [http://2008.igem.org/Beijing\_Normal/16\_August\_2008 16] | [http://2008.igem.org/Beijing\_Normal/17\_August\_2008 17] |
| [http://2008.igem.org/Beijing\_Normal/18\_August\_2008 18] | [http://2008.igem.org/Beijing\_Normal/19\_August\_2008 19] | [http://2008.igem.org/Beijing\_Normal/20\_August\_2008 20] | [http://2008.igem.org/Beijing\_Normal/21\_August\_2008 21] | [http://2008.igem.org/Beijing\_Normal/22\_August\_2008 22] | [http://2008.igem.org/Beijing\_Normal/23\_August\_2008 23] | [http://2008.igem.org/Beijing\_Normal/24\_August\_2008 24] |
| [http://2008.igem.org/Beijing\_Normal/25\_August\_2008 25] | [http://2008.igem.org/Beijing\_Normal/26\_August\_2008 26] | [http://2008.igem.org/Beijing\_Normal/27\_August\_2008 27] | [http://2008.igem.org/Beijing\_Normal/28\_August\_2008 28] | [http://2008.igem.org/Beijing\_Normal/29\_August\_2008 29] | [http://2008.igem.org/Beijing\_Normal/30\_August\_2008 30] | [http://2008.igem.org/Beijing\_Normal/31\_August\_2008 31] |

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| September | | | | | | |
| M | T | W | T | F | S | S |
| [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/1\_September\_2008&action=edit 1] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/2\_September\_2008&action=edit 2] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/3\_September\_2008&action=edit 3] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/4\_September\_2008&action=edit 4] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/5\_September\_2008&action=edit 5] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/6\_September\_2008&action=edit 6] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/7\_September\_2008&action=edit 7] |
| [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/8\_September\_2008&action=edit 8] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/9\_September\_2008&action=edit 9] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/10\_September\_2008&action=edit 10] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/11\_September\_2008&action=edit 11] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/12\_September\_2008&action=edit 12] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/13\_September\_2008&action=edit 13] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/14\_September\_2008&action=edit 14] |
| [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/15\_September\_2008&action=edit 15] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/16\_September\_2008&action=edit 16] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/17\_September\_2008&action=edit 17] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/18\_September\_2008&action=edit 18] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/19\_September\_2008&action=edit 19] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/20\_September\_2008&action=edit 20] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/21\_September\_2008&action=edit 21] |
| [http://2008.igem.org/Beijing\_Normal/22\_September\_2008 22] | [http://2008.igem.org/Beijing\_Normal/23\_September\_2008 23] | [http://2008.igem.org/Beijing\_Normal/24\_September\_2008 24] | [http://2008.igem.org/Beijing\_Normal/25\_September\_2008 25] | [http://2008.igem.org/Beijing\_Normal/26\_September\_2008 26] | [http://2008.igem.org/Beijing\_Normal/27\_September\_2008 27] | [http://2008.igem.org/Beijing\_Normal/28\_September\_2008 28] |
| [http://2008.igem.org/Beijing\_Normal/29\_September\_2008 29] | [http://2008.igem.org/Beijing\_Normal/30\_September\_2008 30] |

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| October | | | | | | |
| M | T | W | T | F | S | S |
|  |  | [http://2008.igem.org/Beijing\_Normal/1\_October\_2008 1] | [http://2008.igem.org/Beijing\_Normal/2\_October\_2008 2] | [http://2008.igem.org/Beijing\_Normal/3\_October\_2008 3] | [http://2008.igem.org/Beijing\_Normal/4\_October\_2008 4] | [http://2008.igem.org/Beijing\_Normal/5\_October\_2008 5] |
| [http://2008.igem.org/Beijing\_Normal/6\_October\_2008 6] | [http://2008.igem.org/Beijing\_Normal/7\_October\_2008 7] | [http://2008.igem.org/Beijing\_Normal/8\_October\_2008 8] | [http://2008.igem.org/Beijing\_Normal/9\_October\_2008 9] | [http://2008.igem.org/Beijing\_Normal/10\_October\_2008 10] | [http://2008.igem.org/Beijing\_Normal/11\_October\_2008 11] | [http://2008.igem.org/Beijing\_Normal/12\_October\_2008 12] |
| [http://2008.igem.org/Beijing\_Normal/13\_October\_2008 13] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/14\_October\_2008&action=edit 14] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/15\_October\_2008&action=edit 15] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/16\_October\_2008&action=edit 16] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/17\_October\_2008&action=edit 17] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/18\_October\_2008&action=edit 18] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/19\_October\_2008&action=edit 19] |
| [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/20\_October\_2008&action=edit 20] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/21\_October\_2008&action=edit 21] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/22\_October\_2008&action=edit 22] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/23\_October\_2008&action=edit 23] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/24\_October\_2008&action=edit 24] | [http://2008.igem.org/Beijing\_Normal/25\_October\_2008 25] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/26\_October\_2008&action=edit 26] |
| [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/27\_October\_2008&action=edit 27] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/28\_October\_2008&action=edit 28] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/29\_October\_2008&action=edit 29] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/30\_October\_2008&action=edit 30] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/31\_October\_2008&action=edit 31] |

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| November | | | | | | |
| M | T | W | T | F | S | S |
|  |  |  |  |  | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/1\_November\_2008&action=edit 1] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/2\_November\_2008&action=edit 2] |
| [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/3\_November\_2008&action=edit 3] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/4\_November\_2008&action=edit 4] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/5\_November\_2008&action=edit 5] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/6\_November\_2008&action=edit 6] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/7\_November\_2008&action=edit 7] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/8\_November\_2008&action=edit 8] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/9\_November\_2008&action=edit 9] |
| [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/10\_November\_2008&action=edit 10] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/11\_November\_2008&action=edit 11] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/12\_November\_2008&action=edit 12] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/13\_November\_2008&action=edit 13] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/14\_November\_2008&action=edit 14] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/15\_November\_2008&action=edit 15] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/16\_November\_2008&action=edit 16] |
| [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/17\_November\_2008&action=edit 17] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/18\_November\_2008&action=edit 18] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/19\_November\_2008&action=edit 19] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/20\_November\_2008&action=edit 20] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/21\_November\_2008&action=edit 21] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/22\_November\_2008&action=edit 22] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/23\_November\_2008&action=edit 23] |
| [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/24\_November\_2008&action=edit 24] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/25\_November\_2008&action=edit 25] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/26\_November\_2008&action=edit 26] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/27\_November\_2008&action=edit 27] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/28\_November\_2008&action=edit 28] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/29\_November\_2008&action=edit 29] | [http://2008.igem.org/wiki/index.php?title=Beijing\_Normal/30\_November\_2008&action=edit 30] |

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