iGEM 2015 — Team ZJU-China

# Team:ZJU-China/Notebook

[![](https://static.igem.org/mediawiki/2015/c/cc/ZJU-2015-logo-red.png)
![](https://static.igem.org/mediawiki/2015/7/75/ZJU-Termi2.png)
![](https://static.igem.org/mediawiki/2015/7/78/IGem-meet-zju.png)](https://2015.igem.org/Team%3AZJU-China)

[![](https://static.igem.org/mediawiki/2015/b/bf/ZJU-HOME.png)](https://2015.igem.org/Team%3AZJU-China)

[Home](https://2015.igem.org/Team%3AZJU-China)

![](https://static.igem.org/mediawiki/2015/a/a0/ZJU-Parts.png)

[Team Part Team Part](https://2015.igem.org/Team%3AZJU-China/Parts)

[Basic Part Basic Part](https://2015.igem.org/Team%3AZJU-China/Basic_Part)

[Improved Part Improved Part](https://2015.igem.org/Team%3AZJU-China/Description)

[Composite Part Composite Part](https://2015.igem.org/Team%3AZJU-China/Composite_Part)

[Parts](https://2015.igem.org/Team%3AZJU-China/Parts)

![](https://static.igem.org/mediawiki/2015/d/dd/ZJU-Project.png)

[Overview Quick view for our project](https://2015.igem.org/Team%3AZJU-China/Project)
 [Termite Issue The Termite Issue](https://2015.igem.org/Team%3AZJU-China/Project/Termite_Issue)

Design

* [Toxins Manufacture](https://2015.igem.org/Team%3AZJU-China/Design/Toxinmanufacture)
* [Bacteria Carrier](https://2015.igem.org/Team%3AZJU-China/Design/CNC)
* [Termites](https://2015.igem.org/Team%3AZJU-China/Design/Termites)

[Protocol Quick view for Protocol](https://2015.igem.org/Team%3AZJU-China/Project/Protocol)

[Project](https://2015.igem.org/Team%3AZJU-China/Project)

![](https://static.igem.org/mediawiki/2015/9/96/ZJU-Outreach.png)

[Overview Quick view for Outreach](https://2015.igem.org/Team%3AZJU-China/Practices)

Society

* [Survey](https://2015.igem.org/Team%3AZJU-China/Practices/Society/Survey)
* [Consultation](https://2015.igem.org/Team%3AZJU-China/Practices/Society/Consultation)
* [Syn-bio Debate](https://2015.igem.org/Team%3AZJU-China/Practices/Society/Syn-bio_Debate)

Application

* [Device](https://2015.igem.org/Team%3AZJU-China/Design)
* [Promotion](https://2015.igem.org/Team%3AZJU-China/Entrepreneurship)
* [Safety](https://2015.igem.org/Team%3AZJU-China/Practices/Application/Safety)
* [Future](https://2015.igem.org/Team%3AZJU-China/Practices/Application/Future)

Public Promotion

* [Poly-cards](https://2015.igem.org/Team%3AZJU-China/Practices/Public_Promotion/Poly-cards)
* [Syn-bio Share](https://2015.igem.org/Team%3AZJU-China/Practices/Public_Promotion/Syn-bio_Share)
* [Community Lab](https://2015.igem.org/Team%3AZJU-China/Practices/Public_Promotion/Community_Lab)
* [Seminar](https://2015.igem.org/Team%3AZJU-China/Practices/Public_Promotion/Seminar)

Collaboration

* [SynenergeneⅠ](https://2015.igem.org/Team%3AZJU-China/Practices/Cooperate/Senergene/first)
* [SynenergeneⅡ](https://2015.igem.org/Team%3AZJU-China/Practices/Cooperate/Senergene/second)
* [Synenergene Ⅲ](https://2015.igem.org/Team%3AZJU-China/Practices/Cooperate/Senergene/third)
* [Other Teams](https://2015.igem.org/Team%3AZJU-China/Collaborations)

[Outreach](https://2015.igem.org/Team%3AZJU-China/Practices)

![](https://static.igem.org/mediawiki/2015/2/2d/ZJU-Team.png)

[Members Introduce our Members](https://2015.igem.org/Team%3AZJU-China/Team)

[Attributions Introduce the Attributions](https://2015.igem.org/Team%3AZJU-China/Attributions)

[Acknowledgement Introduce the Acknowledgement](https://2015.igem.org/Team%3AZJU-China/Team/Acknowledgement)

[Sponsors Introduce our Sponsors](https://2015.igem.org/Team%3AZJU-China/Team/Sponsors)

[Team](https://2015.igem.org/Team%3AZJU-China/Team)

![](https://static.igem.org/mediawiki/2015/9/94/ZJU-Model.png)

[Modeling Overview Introduce our Members](https://2015.igem.org/Team%3AZJU-China/Modeling)

[Modeling α Termite Simulation](https://2015.igem.org/Team%3AZJU-China/Modeling/first)

[Modeling β Mass transfer model](https://2015.igem.org/Team%3AZJU-China/Modeling/second)

[Modeling γ Degradation model](https://2015.igem.org/Team%3AZJU-China/Modeling/third)

[Modeling](https://2015.igem.org/Team%3AZJU-China/Modeling)

[![](https://static.igem.org/mediawiki/2015/9/90/ZJU-Notebook.png)](https://2015.igem.org/Team%3AZJU-China/Notebook)

[Notebook](https://2015.igem.org/Team%3AZJU-China/Notebook)

[![](https://static.igem.org/mediawiki/2015/4/45/ZJU-Result.png)](https://2015.igem.org/Team%3AZJU-China/Results)

[Experiment Results toxins manufacture](https://2015.igem.org/Team%3AZJU-China/Results)

[Experiment Result bacteria carrier](https://2015.igem.org/Team%3AZJU-China/Results/second)

[Experiment Result termites simulation](https://2015.igem.org/Team%3AZJU-China/Results/third)

[Social Result social results](https://2015.igem.org/Team%3AZJU-China/Results/social_result)

[Results](https://2015.igem.org/Team%3AZJU-China/Results)

[![](https://static.igem.org/mediawiki/2015/6/6f/ZJU-Safety.png)](https://2015.igem.org/Team%3AZJU-China/Safety)

[Safety](https://2015.igem.org/Team%3AZJU-China/Safety)

![](https://static.igem.org/mediawiki/2015/9/96/ZJU-Application.png)

[Device Introduce our Device and Instruction Manual](https://2015.igem.org/Team%3AZJU-China/Design)

[Promotion The Promotion](https://2015.igem.org/Team%3AZJU-China/Entrepreneurship)

[Safety The Safety](https://2015.igem.org/Team%3AZJU-China/Practices/Application/Safety)

[Future Future](https://2015.igem.org/Team%3AZJU-China/Practices/Application/Future)

[Application](https://2015.igem.org/Team%3AZJU-China/Design)

![](https://static.igem.org/mediawiki/2015/a/a7/ZJU-CHINA_AjaxLoader.gif)

## Notebook

We listed 23 pieces of Breaking News. Here are the links:

[* ZJU-China 2015 team was set up](#event1)
[* Brainstorm began.](#e1   )
[* Decided our project: termite terminator.](#e3   )
[* Successfully got the solution of CNC!!](#e4   )
[* Got the genome of tcdA1 and tcdB1 in NCBI.](#e6   )
[* iGEM Shanghai Tour.](#e9   )
[* Got the solid CNC with lyophilization.](#e10  )
[* Solved the diffusion equation.](#e14  )
[* Attended NCTU meetup in Taiwan.](#e15  )
[* Discussed helping BNU and HUST.](#e16  )
[* Attached more importance to safety.](#e18  )
[* Got our device for detecting and killing termites.](#e22  )

Croll down to see more.

* September
* 9.17.2015

  One of our member is practicing Shuffle~ He and his partner will have a great show in Giant Jamboree :)

  ![](https://static.igem.org/mediawiki/2015/thumb/7/75/ZJU-CHINA_9.18_%281%29.jpg/675px-ZJU-CHINA_9.18_%281%29.jpg)
* 9.5.2015

  Finished the overview of entire project.

  ![](https://static.igem.org/mediawiki/2015/thumb/3/3d/9-5.jpg/800px-9-5.jpg)
* 9.4.2015

  + Bacteria solution PCR: OrfX. One positive clone of 20 samples.
  + DNA sequencing: OrfX.
* 9.2.2015

  Dynamic Light Scattering(DLS) experiment to measure the particle size of embedded E.coli.

  ![](https://static.igem.org/mediawiki/2015/thumb/d/d5/9-2.jpg/800px-9-2.jpg)
* 9.1.2015

  The sequence of OrfX was not correct because of the wrong template, thus we had to do the related experiments again.
* August
* 8.31.2015

  Took photos and prepared for the banner~

  ![](https://static.igem.org/mediawiki/2015/thumb/8/87/8-31-2.jpg/450px-8-31-2.jpg)

  ![](https://static.igem.org/mediawiki/2015/thumb/e/e7/8-31.jpg/800px-8-31.jpg)
* 8.30.2015

  The sequence of metK and tcdA1 were confirmed, which marked the success in standardization of 2 parts!!
* 8.29.2015

  Bacteria solution PCR: metK and OrfX. Both had positive clones.
  DNA sequencing: metK and OrfX.
  T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3.
  Selected from single colonies: CDS-tcdA1.
* 8.28.2015

  + Selected from single colonies of metK and OrfX.
  + Submit the safety form. Selected from single colonies: CDS-plu0840. Double enzyme digestion and DNA gel extraction: plu1537, tcdA1, plu0840. Plasmid extration and double enzyme digestion: RFP. Protein extraction and SDS-PAGE: tcdA1, plu1537, plu0840. T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3. Bacteria solution PCR: CDS-plu0840.
* 8.27.2015

  Seamless assembly: plasmid+OrfX, plasmid+metK. PCR: CDS-tcdA1. Double enzyme digestion: RFP+plasmid. Seamless assembly: CDS-plu0840. PCR and DNA gel extraction: CDS-tcdA1. Changed pSB1A3 to pSB1C3: plu0840, plu1537, tcdA1. Transformed them into E.coli DH5α, selected from single colonies and used single enzyme digestion to verify the got plasmids.
* 8.26.2015

  Seamless assembly: plasmid+CDS-tcdA1, plasmid+CDS-plu0840. DNA gel extraction: tcdA1. PCR: device-plu0840.
* 8.25.2015

  The sequence of ermE+plasmid, Frr+plasmid and plu1537+plasmid were confirmed, which marked the success in standardization of 3 parts!!
* 8.24.2015

  + Bacteria solution PCR: metK and OrfX. Only negative clone. Extraction of plasmid mCherry and double enzyme digestion. DNA sequencing: ermE+plasmid, Frr+plasmid, plu1537+plasmid. Selected from single colonies of plu1537.
  + Power cut. We played table tennis near our lab.

  ![](https://static.igem.org/mediawiki/2015/thumb/5/58/8-24.png/800px-8-24.png)
* 8.23.2015

  + Selected from single colonies of metK and OrfX.
  + Transformed standard plu1537 plasmid into BL-21. Bacterium solution PCR: ermE and Frr.
  + We kept a Madagascar cockroach as our pet~

  ![](https://static.igem.org/mediawiki/2015/thumb/5/5c/8-23.jpg/450px-8-23.jpg)

  ![](https://static.igem.org/mediawiki/2015/thumb/0/06/8-23-3.jpg/800px-8-23-3.jpg)
* 8.22.2015

  + Selected from single colonies of ermE and Frr. Extraction of plasmid (standard plu1537).
  + Bacterium solution PCR: standard plu1537.
* 8.21.2015

  + DNA sequencing. Seamless assembly: plasmid+1537, plasmid+tcdA1-L+ tcdA1-M+ tcdA1-R, plasmid+ermE, plasmid+Frr.
  + Successfully booked the HI-Boston Hostel and paid the full payment~ Seamless assembly: plasmid+metK, plasmid+OrfX.
* #### 38th Group meeting

  8.20.2015

  38th Group meeting. Introduced a software named Mou which could enhance the efficiency of writing wiki. Discussed the business planning of our product and the photos on the wiki.
* 8.20.2015

  + Extraction of plasmid mCherry and double enzyme digestion. Seamless assembly: plasmid+1537, plasmid+tcdB1. PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction.
  + Capsulated Streptomyces Avermitilis toxicity test. TT01 toxicity verification.
  + PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction.
  + Again used flow cytometry to check the diameter of the embedded E.coli and tried to count the E.coli which were successfully embedded. The results were not that ideal again.
* 8.19.2015

  + PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction.
  + Finished the background of avermectin and toxic protein. Sent bacterium sample to BNU and we would continue to help them.

  ![](https://static.igem.org/mediawiki/2015/thumb/c/c6/8-19.jpg/800px-8-19.jpg)
* 8.18.2015

  + PCR: CDS tcdB1, CDS 1537, tcdA1-L, mcherry. DNA sequencing.
  + Half-done the model of degration of toxic protein. Got the kit for detection avermectin.
* 8.17.2015

  + PCR: CDS tcdB1, CDS plu1537 and mCherry. DNA gel extraction: tcdA1-L and plu0840L. Seamless assembly: plasmid+pBad+plu1537.
  + Detected the relationship between distance of food source and food consumption.
  + Designed the primers for Frr, metK, OrfX and ermE again because the need for standardization.
* #### 37th Group meeting

  8.16.2015

  37th Group meeting. Built the framework for wiki. Discussed the Syn-bio talks on next Saturday.
* 8.16.2015

  + Preparation of competent cells.DNA gel extraction.
  + Verification of the bait attractiveness.
* 8.15.2015

  + PCR: pBad, tcdA1-L, tcdB1, plu0840L and plu1537. Purchased E.coli BL21.
  + The experiment concerning Streptomyces avermitilis finished 80%.
  + Lovely poppy~

  ![](https://static.igem.org/mediawiki/2015/thumb/c/cd/8-15.jpg/450px-8-15.jpg)
* 8.14.2015

  + PCR and clean up. Concluded the successful PCR parameters of different gene clusters.
  + Test the Avermectin detection kit with pure Ivermectin.
  + Designed the primers for plu0840, plu1537, tcdA1 and tcdB1 again bucause of the need for standardization.

  ![](https://static.igem.org/mediawiki/2015/thumb/a/a3/%E5%B1%8F%E5%B9%95%E5%BF%AB%E7%85%A7_2015-09-08_%E4%B8%8B%E5%8D%8811.50.11.png/800px-%E5%B1%8F%E5%B9%95%E5%BF%AB%E7%85%A7_2015-09-08_%E4%B8%8B%E5%8D%8811.50.11.png)
* 8.13.2015

  + Overlap: pBad and tcdA1-L, pBad and tcd-B1, pBad and plu1537. Extraction of plasmid mCherry and enzyme digestion.
  + Completed wiki page for 2nd model. Drew the 3D ball-and-stick models for CNC with Solidworks. Completed the device for killing termites.
  + Yummy hotpot~
  + Completed the SCM program for HAZU and they affirmed our help.

  ![](https://static.igem.org/mediawiki/2015/0/0e/8-13.jpg)

  ![](https://static.igem.org/mediawiki/2015/thumb/3/3b/8-13-2.jpg/450px-8-13-2.jpg)
* 8.12.2015

  + Overlap: 0840L and 0840R.
  + Tried to purify the embedded E.coli with the E.coli which were able to express RFP.
  + Used flow cytometry to check the diameter of the embedded E.coli and tried to count the E.coli which were successfully embedded. The results were not that ideal.

  ![](https://static.igem.org/mediawiki/2015/thumb/d/d3/%E6%B5%81%E5%BC%8F.png/800px-%E6%B5%81%E5%BC%8F.png)
* 8.11.2015

  + PCR: pBad, tcdA1-L, tcdA1-M, tcdA1-R, tcdB1, 0840L, 0840R, 1537, GFP.
  + PCR: ligated product. Optimized the PCR program. Cleaned up. PCR again.
  + SEM scanned our samples to verify whether Streptomyces avermitilis were successfully embedded with CNC.
  + Fed the termites with Photorhabdus luminescens TT01 bacterium cells.

  ![](https://static.igem.org/mediawiki/2015/4/4c/8-11.png)
* 8.10.2015

  + PCR: pBad, tcdA1-L and GFP.
  + Used Bgl2 and EcoR1 digested inserted fragment and cleaned up the product. Ligating fragment into plasmid with T4 ligase.
  + Specified the standardization requirements and laid the foundation for primer designing.
  + Mixed blue-stained termites with red-stained termites to verify trophallaxis.

  ![](https://static.igem.org/mediawiki/2015/thumb/f/f6/8-10.png/470px-8-10.png)
* #### 35th Group meeting

  8.9.2015

  35th Group meeting. Planned to hold a seminar for freshmen. Tried to design a device for attracting and killing termites.
* 8.9.2015

  + PCR: tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R and plu1537.
  + Completed our framework and philosophy of safety.
  + Extracted the plasmid containing RFP from DH5α. PCR: RFP and confirmed by gel electrophoresis.

  ![](https://static.igem.org/mediawiki/2015/thumb/3/3f/8-9.jpg/800px-8-9.jpg)
* 8.8.2015

  Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).

  ![](https://static.igem.org/mediawiki/2015/thumb/3/38/8-8-2.png/800px-8-8-2.png)
* 8.8.2015

  Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).

  ![](https://static.igem.org/mediawiki/2015/thumb/b/bb/8-8-3.jpg/800px-8-8-3.jpg)
  ![](https://static.igem.org/mediawiki/2015/thumb/8/8f/8-8.jpeg/800px-8-8.jpeg)
* 8.7.2015

  One step cloning protocol finished. Designed the questionaire about termites.
* 8.7.2015-8.8.2015

  PCR: pBad, GFP, tcdA1-M, tcdA1-R.
* #### 34th Group meeting

  8.6.2015

  34th Group meeting. Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety.
* 8.6.2015

  + Drawing plasmid circuits in snapgene.(toxic protein)
  + Stained 50 termites in blue and 50 in red for trophallaxis experiments.

  ![](https://static.igem.org/mediawiki/2015/thumb/e/ed/8-6.png/800px-8-6.png)
* 8.5.2015

  + Discussed the circuit construction and experiments planning of toxic protein.
  + Decided to give up confocol because we couldn't find the specific dye for CNC.

  ![](https://static.igem.org/mediawiki/2015/thumb/c/c7/8-5.jpg/450px-8-5.jpg)
* 8.4.2015

  Completed the One Step Cloning protocol.

  ![](https://static.igem.org/mediawiki/2015/thumb/e/e1/8-4.png/494px-8-4.png)
* #### 33rd Group meeting

  8.2.2015

  33rd Group meeting. Began to build framework for wiki. Introduced the habits and characteristics of termites.
* July
* #### 32nd Group meeting

  7.30.2015

  32nd Group meeting. Planned to promote synthetic biology and our project in Shanghai Science & Technology Museum. Polished up the Synbio-cards(Polypoly card).
* 7.28.2015-8.4.2015

  PCR: pBad, tcdA1-L, tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R, plu1537 and GFP.
* 7.28.2015

  Giant Jamboree registration completed.

  ![](https://static.igem.org/mediawiki/2015/thumb/e/e5/7-28.png/420px-7-28.png)
* 7.26.2015

  + Listed 15 main problems we were confronted with, including safety, communication between dry group and wet groups.
  + Designed a new method to embed the bacterium with CNC.
* 7.25.2015

  Designed the primers for plu0840, plu1537, tcdA1 and tcdB1 again bucause of the bug of extra RBS.
* 7.24.2015

  Discussed helping BNU and HUST. Designed and alpha tested the Synbio-cards. Improved the modeling.
* 7.19.2015-7.24.2015

  Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST.

  ![](https://static.igem.org/mediawiki/2015/thumb/b/bc/7-20.jpg/800px-7-20.jpg)
* 7.19.2015-7.24.2015

  Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST.

  ![](https://static.igem.org/mediawiki/2015/thumb/3/3b/7-19.jpg/800px-7-19.jpg)
* 7.18.2015

  + Listed and detailed the wiki requirements.
  + Fed the termites with Streptomyces Avermitilis bacterium cells.
* 7.16.2015

  Prepared the home page and member page of our wiki. Prepared the poster for NCTU meetup.

  ![](https://static.igem.org/mediawiki/2015/thumb/4/42/7-16.png/338px-7-16.png)
* 7.15.2015

  + Designed the primers for Frr, metK, OrfX and ermE again. Got our name card~
  + Used confocal to verify the successful package of E.coli with CNC. However, the result appeared not ideal and we couldn't find appropriate dye of CNC.

  ![](https://static.igem.org/mediawiki/2015/5/51/7-15.png)
* 7.14.2015

  Prepared for the souvenirs for NCTU meetup.
* #### 31st Group meeting

  7.12.2015

  31st Group meeting. Reviewed the medal requirement and all the rules in lab.

  ![](https://static.igem.org/mediawiki/2015/thumb/e/e8/7-12.png/605px-7-12.png)
* 7.11.2015

  Mark-recapture method failed because we failed to recapture enough stained termites.
* 7.3.2015-7.11.2015

  Exams of summer semester.
* June
* #### 30th Group meeting

  6.25.2015

  30th Group meeting. Planned to verify the package of CNC with confocal. Continued to prepare for the presentation and application scenario.
* 6.24.2015

  + Completed 2 safety questionaires.
  + Prepared to learn confocol to verify the CNC package for E.coli.
* 6.22.2015

  Put 150 blue stained termites back into the nest for mark-recapture method.
* #### 29th Group meeting

  6.21.2015

  29th Group meeting. Discussed the application scenario for the 5000€ additional sponsership of SYNENERGENE.
* 6.21.2015

  Contacted with Björn and he would help us animate the process of crystallization.
* 6.20.2015

  TEM scanned our samples.
* 6.19.2015

  Fed the termites with Photorhabdus luminescens TT01 bacterium solution to test the natural toxicity.
  Stained 150 termites in blue for future experiment.
* #### 28th Group meeting

  6.18.2015

  28th Group meeting. 2nd modeling half done. Discussed the results of the SEM.
* 6.17.2015

  TEM and SEM scanned our samples.

  ![](https://static.igem.org/mediawiki/2015/thumb/8/8a/6-17-3.png/798px-6-17-3.png)
* 6.17.2015

  TEM and SEM scanned our samples.

  ![](https://static.igem.org/mediawiki/2015/thumb/7/74/6-17-2.jpg/590px-6-17-2.jpg)
  ![](https://static.igem.org/mediawiki/2015/a/a4/6-17.jpg)
* 6.15.2015

  Fed the termites with Streptomyces Avermitilis bacterium solution to test the natural toxicity.
* #### 27th Group meeting

  6.14.2015

  27th Group meeting. Rearranged all the events with four quadrant method. Checked everything we have had.

  ![](https://static.igem.org/mediawiki/2015/thumb/7/70/6-14.jpg/800px-6-14.jpg)
* 6.14.2015

  Solved the diffusion equation.

  ![](https://static.igem.org/mediawiki/2015/3/3d/6-14-2.jpg)

  ![](https://static.igem.org/mediawiki/2015/thumb/b/ba/6-14-3.png/800px-6-14-3.png)
* 6.13.2015

  Extracted the genome of Streptomyces avermitilis.
* 6.12.2015

  Went to shanghai for the interview of visa. 12 members successfully passed the interview.
* #### 26th Group meeting

  6.11.2015

  26th Group meeting. Discussed the half-done presentation and Skype conference with SYNENERGENE. Prepared for the visa.
* 6.11.2015

  PCR: plu1537, with the template of the genome of TT01.
* 6.10.2015

  + Paid for the air tickets.
  + Got the primers of toxic protein.
* 6.8.2015

  Optimization of the model of termites finding food.

  ![](https://static.igem.org/mediawiki/2015/8/82/6-8-2.png)

  ![](https://static.igem.org/mediawiki/2015/thumb/2/27/6-8-3.png/800px-6-8-3.png)

  ![](https://static.igem.org/mediawiki/2015/1/1b/6-8.png)
* #### 25th Group meeting

  6.7.2015

  25th Group meeting. Polished up the previous modeling. Protocols about the experiments concerning bacterium. A member quit.
* 6.7.2015

  Extracted the genome of TT01.
* 6.6.2015

  Frozen the spores of Streptomyces avermitilis.

  Judge Haoqian Zhang from Peking University came to our lab. Frozen the spores of Streptomyces avermitilis.

  ![](https://static.igem.org/mediawiki/2015/thumb/0/03/6-6.jpeg/800px-6-6.jpeg)
* #### 24th Group meeting

  6.4.2015

  24th Group meeting. Brainstormed the Synbio-cards and Synbio-workshops. Listed the datailed personal scheme about the time arrangement before summer holiday.

  ![](https://static.igem.org/mediawiki/2015/thumb/9/98/6-4-2.png/525px-6-4-2.png)
  ![](https://static.igem.org/mediawiki/2015/thumb/4/41/6-4.jpg/692px-6-4.jpg)
* 6.3.2015

  + Discussed the characterization of E.coli embedded in CNC, including TEM, SEM, confocal, FTIR and flow cytometry.
  + Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE! Cheers~
  + Brainstormed how to do human practice and use the €5000. We came up with the Synbio-cards and Synbio-workshops.

  ![](https://static.igem.org/mediawiki/2015/thumb/8/8d/6-3.png/800px-6-3.png)
* 6.2.2015

  Began to book the air tickets. Booked the first primers of toxic protein for one step cloning. Found the sequence of the gene we need in the Streptomyces avermitilis.
* 6.1.2015

  SEM scanned our samples again. Debugged the first modeling.
* May
* #### 23rd Group meeting

  5.31.2015

  23rd Group meeting. Discussed the half-done modeling.
* 5.31.2015

  Built up the model of termites finding food.

  ![](https://static.igem.org/mediawiki/2015/7/7a/5-31-2.png)
* 5.31.2015

  Taught high school students to do experiment to extract DNA from epithelial cell of mouth.

  ![](https://static.igem.org/mediawiki/2015/thumb/b/b7/5-31-3.jpg/800px-5-31-3.jpg)

  ![](https://static.igem.org/mediawiki/2015/thumb/f/f5/5-31-2.jpg/800px-5-31-2.jpg)

  ![](https://static.igem.org/mediawiki/2015/thumb/7/78/5-31.jpg/800px-5-31.jpg)
* 5.29.2015

  + Repeated the cellulase activity detection experiment.
    Meanwhile we measured the lysozyme activity in different parts of termites guts.
  + 22nd Group meeting. Specified the medal requirement. Began to prepare for the NCTU meetup.
  + SEM scanned our samples.
* 5.28.2015

  Completed a model of a cartoon termite holding a sword. Cleaned up our lab and rearranged all the experiment materials we would need.
* 5.26.2015

  Got the solid CNC with lyophilization. Built the framework of the first web page.

  ![](https://static.igem.org/mediawiki/2015/thumb/0/0b/5-26.png/800px-5-26.png)
* 5.25.2015

  Learned the skills of culturing Streptomyces avermitilis in the professor's lab.
* #### 21st Group meeting

  5.24.2015

  21st Group meeting. Began to prepare for the presentation.
* 5.22.2015

  iGEM Shanghai Tour. Took part in the conference held in NYU. Visited FDU and communicated with them.
* ####

  5.21.2015

  Chosen the certain toxic protein which we would transfer into E.coli: tcdA1, tcdB1,plu0840 and plu1537.
* 5.21.2015

  20th Group meeting. Discussed the medal requirements and collaboration among groups.
* 5.20.2015

  + Finished the protocol about TT01.
  + Measured the cellulase activity in termites head, forgut, midgut and hindgut.
* 5.19.2015

  Detailed the medal requirements depending on our project. Finished the protocol about Streptomyces avermitilis.

  ![](https://static.igem.org/mediawiki/2015/thumb/8/89/5-19-2.png/800px-5-19-2.png)

  ![](https://static.igem.org/mediawiki/2015/thumb/8/89/5-19-2.png/800px-5-19-2.png)
* 5.18.2015

  Got the E.coli strain ET12567 from Professor Li's Lab. Began the experiment about Streptomyces avermitilis.
* #### 18th Group meeting

  5.17.2015

  18th Group meeting. Emphasized the lab safety.
* 5.17.2015

  Did experiments(rainbow kit)related to synthetic biology in Zhejiang Science&Technology Museum with high school students and by this way promoted syn-bio.

  ![](https://static.igem.org/mediawiki/2015/thumb/a/a3/5-17.jpg/800px-5-17.jpg)
* 5.15.2015

  Read paper and discussed how to enhance the efficiency of Avermectin in Streptomyces avermitilis.

  ![](https://static.igem.org/mediawiki/2015/thumb/4/4a/5-15.png/800px-5-15.png)
* #### 17th Group meeting

  5.14.2015

  17th Group meeting. Discussed the results of the TEM. Tried to contact with a judge from PKU.
* 5.14.2015

  + TEM scanned our samples: using CNC to pack the Bacillus subtilis.
  + Completed the cover and comic of our proposal which would be handed in to SYNENERGENE.
  + Contacted with Hangzhou Termites control Institute.

  ![](https://static.igem.org/mediawiki/2015/thumb/f/f4/5-14-3.jpg/800px-5-14-3.jpg)
  ![](https://static.igem.org/mediawiki/2015/thumb/4/48/5-14.jpg/590px-5-14.jpg)
* 5.14.2015

  + TEM scanned our samples: using CNC to pack the Bacillus subtilis.
  + Completed the cover and comic of our proposal which would be handed in to SYNENERGENE.
  + Contacted with Hangzhou Termites control Institute.

  ![](https://static.igem.org/mediawiki/2015/thumb/0/06/5-14-2.jpg/590px-5-14-2.jpg)
* 5.13.2015

  + Got the genome of tcdA1 and tcdB1 in NCBI.
  + Got TT01(a species of Photorhabdus luminescens) which could produce toxic protein. It would play an important role in our plan B.

  ![](https://static.igem.org/mediawiki/2015/thumb/c/c3/5-13.JPG/800px-5-13.JPG)
* 5.12.2015

  Successfully checked in termites and tc protein family.

  ![](https://static.igem.org/mediawiki/2015/0/02/5-12.png)
* #### 16th Group meeting

  5.10.2015

  16th Group meeting. Communication between dry group and 3 wet groups. Arranged time for experiments.
* #### 15th Group meeting

  5.7.2015

  15th Group meeting. Further discussed the proposal and allocated the tasks. Continued to consult professors about several species of bacterium.
* 5.6.2015

  Gave up synthesizing avermectin in Bacillus subtilis. Tried to overexpress avermectin in E.coli and then transformed its plasmid into Streptomyces avermitilis.

  ![](https://static.igem.org/mediawiki/2015/thumb/e/e4/5-6-2.jpg/800px-5-6-2.jpg)
* 5.6.2015

  Successfully got the solution of CNC!! And it appeared Tyndall effect.

  ![](https://static.igem.org/mediawiki/2015/thumb/c/c1/5-6.jpg/800px-5-6.jpg)
* 5.4.2015-5.6.2015

  Exams of spring semester.
* 5.3.2015

  Our termite nest was sent out from Guangdong Province.
* April
* #### 14th Group meeting

  4.29.2015

  14th Group meeting. 10 members would go to Taiwan for NCTU meetup. Results of consulting professors specialized in termites and Bacillus subtilis.

  ![](https://static.igem.org/mediawiki/2015/a/ad/4-29.png)
* 4.28.2015

  + Discussed the plan for embedding baterium with CNC and the self-assembly of CMC and chitosan on the surface of baterium.
  + Debugged the problem of synthesizing avermectin in Streptomyces avermitilis. Consulted professors specialized in synthesizing avermectin in Streptomyces avermitilis.
* 4.27.2015

  Consulted professors specialized in termites and Bacillus subtilis.
* #### 13th Group meeting

  4.26.2015

  13th Group meeting. Discussed something important: the proposal for the 5000€ additional sponsership of SYNENERGENE and visa. A new mamber joined our team. Specified the problem remaining to be solved of each group.
* 4.25.2015

  Discussd the advantage of Bacillus subtilis campared with E.coli and the utilization of spore of Bacillus subtilis.
* #### 12th Group meeting

  4.23.2015

  12th Group meeting. Debugged the previous package plan and decided to pack bacterium with CNC. Planned to buy a nest of termites from Guangdong Province. Discussed several demensions of modeling in this project.

  ![](https://static.igem.org/mediawiki/2015/thumb/a/a0/4-23-2.jpg/600px-4-23-2.jpg)

  ![](https://static.igem.org/mediawiki/2015/thumb/a/a6/4-23.jpg/450px-4-23.jpg)
* 4.22.2015

  Consulted professors specialized in termites.
* 4.21.2015

  Consulted professors about directly embedding baterium with CNC rather than with the aid of protein domain. Our idea was confirmed and praised.

  ![](https://static.igem.org/mediawiki/2015/thumb/8/81/4-21.png/450px-4-21.png)
* 4.20.2015

  Communication with our instructor. Searched for information about cellulose binding domain (CBDs) and overexpression of ivermectin and avermectin.

  ![](https://static.igem.org/mediawiki/2015/thumb/1/18/4-20.png/553px-4-20.png)
* #### 11th Group meeting

  4.19.2015

  11th Group meeting. Decided our project: termite terminator. Allocated all members into 4 groups. Each group was responsible for a part of our project.

  ![](https://static.igem.org/mediawiki/2015/thumb/c/c0/4-19.jpg/800px-4-19.jpg)
* #### 10th Group meeting

  4.16.2015

  10th Group meeting. Decided to participate in NCTU meetup in summer holiday and apply for the 5000€ additional sponsership of SYNENERGENE. Discussed 3 existing ideas.
* 4.14.2015

  Preliminarily designed the circuit in Streptomyces avermitilis.

  ![](https://static.igem.org/mediawiki/2015/thumb/3/35/4-14.jpg/800px-4-14.jpg)
* #### 9th Group meeting

  4.12.2015

  9th Group meeting. Kept 3 ideas and the final idea would derive from them. Detailed these 3 ideas from all the aspects.

  ![](https://static.igem.org/mediawiki/2015/3/34/4-12.jpg)
* #### 8th Group meeting

  4.9.2015

  8th Group meeting. Continued to kill unappropriate ideas. Recalled the aim of participating iGEM and reached a consensus.
* #### 7th Group meeting

  4.6.2015

  7th Group meeting. Began to kill some undoable ideas and further dug the existing ideas, such as biological enigma machine and killing termites.

  ![](https://static.igem.org/mediawiki/2015/1/10/4-6-2.png)
  ![](https://static.igem.org/mediawiki/2015/thumb/c/cf/4-6.png/800px-4-6.png)
* #### 6th Group meeting

  4.2.2015

  6th Group meeting. Developed several new ideas, including bio-SCM, biological enigma machine and slow release of certain chemical. Talked about their feasibility.

  ![](https://static.igem.org/mediawiki/2015/thumb/5/5f/4-2-2.jpg/800px-4-2-2.jpg)

  ![](https://static.igem.org/mediawiki/2015/f/f3/4-2.png)
* March
* #### 5th Group meeting

  3.29.2015

  5th Group meeting. Dug previous ideas and considered their safety and feasibility.
* #### 4th Group meeting

  3.26.2015

  4th Group meeting. Continued to share and improve our ideas, such as bio-lens, detection of schistosomiasis and killing termites.
* 3.25.2015

  Team application completed.

  ![](https://static.igem.org/mediawiki/2015/thumb/0/04/3-25.png/431px-3-25.png)
* #### 3rd Group meeting

  3.22.2015

  3rd Group meeting. Discussed some ideas about modeling. Shared new ideas.
* 3.21.2015

  Communicate with Zhejiang Science&Technology Museum.
* #### 2nd Group meeting

  3.19.2015

  2nd Group meeting. Built up several rules and principles. Tried to make the most of our BBS.
* #### 1st Group meeting

  3.16.2015

  1st Group meeting. Everyone was assigned with his/her own mission. Brainstorm began.
* 3.15.2015

  Visited the AST space of Zhejiang Science&Technology Museum. Decided to collaborate with.
* 3.13.2015

  Concluded the winter project.
* Winter
* 2.1.2015-3.13.2015

  Everyone read wikis, learned something from previous projects and attempted to come up with new ideas.
* 1.31.2015

  1st meetup. Had a nice meal~

  ![](https://static.igem.org/mediawiki/2015/thumb/7/78/1-31.jpg/800px-1-31.jpg)
* 1.30.2015

  15 members was picked out. ZJU-China 2015 team was set up~ Our story began.

  ![](https://static.igem.org/mediawiki/2015/c/c8/1-30-2.jpg)