iGEM 2020 — Team HZAU-China

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:HZAU-China/Notebook

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:HZAU-China/Engineering

# [![点击我返回主页](https://static.igem.org/mediawiki/2020/1/13/T--HZAU-China--logo2.png)](https://2020.igem.org/Team%3AHZAU-China)

- Project
  * [Description](https://2020.igem.org/Team%3AHZAU-China/Description)
  * [Design](https://2020.igem.org/Team%3AHZAU-China/Design)
  * [Implementation](https://2020.igem.org/Team%3AHZAU-China/Implementation)
  * [Proof\_Of\_Concept](https://2020.igem.org/Team%3AHZAU-China/Proof_Of_Concept)
- Wetlab
  * [Safety](https://2020.igem.org/Team%3AHZAU-China/Safety)
  * [Protocol](https://2020.igem.org/Team%3AHZAU-China/Protocol)
  * [Parts](https://2020.igem.org/Team%3AHZAU-China/Parts)
  * [Contribution](https://2020.igem.org/Team%3AHZAU-China/Contribution)
  * [Engineering](https://2020.igem.org/Team%3AHZAU-China/Engineering)
  * [Improvement](https://2020.igem.org/Team%3AHZAU-China/Improvement)
- Drylab
  * [Model](https://2020.igem.org/Team%3AHZAU-China/Model)
  * [Hardware](https://2020.igem.org/Team%3AHZAU-China/Hardware)
  * [Software](https://2020.igem.org/Team%3AHZAU-China/Software)
- Human Practice
  * [Human\_Practices](https://2020.igem.org/Team%3AHZAU-China/Human_Practices)
  * [Collaborations](https://2020.igem.org/Team%3AHZAU-China/Collaborations)
  * [Partnership](https://2020.igem.org/Team%3AHZAU-China/Partnership)
- About us
  * [Team](https://2020.igem.org/Team%3AHZAU-China/Team)
  * [Attributions](https://2020.igem.org/Team%3AHZAU-China/Attributions)

LRAP

ClyR

ClyR fused with PhoA signal peptide

![](https://static.igem.org/mediawiki/2020/f/f9/T--HZAU-China--fairy.png)

# Engineering

This year, we have summited two new parts to the registry to prove our Engineering Success.

# LRAP

LRAP can self-assemble into a chain structure composed of nanospheres by relying on its C-terminal domain, and the presence of calcium ions can enhance its ability. Non-phosphorylated LRAP tends to induce the formation of ordered hydroxyapatite (HA), while phosphorylated LRAP tend to stabilize amorphous calcium phosphate (ACP) and prevent their conversion to HA in vitro. So the non-phosphorylation LRAP are sufficient to transform ACP into ordered bundles of apatite crystals.We also found that LRAP has the ability to bind to natural teeth.

[http://parts.igem.org/Part:BBa\_K3378888](http://parts.igem.org/Part%3ABBa_K3378888)

# ClyR

This chimeric lysin can bind to *S. mutans* by a cell-wall binding domain and then lysis its cell wall from outside by a catalytic domain. ClyR has no activity for Gram-negative bacteria and can be expressed in Escherichia coli. With the guidance of appropriate signal peptide, ClyR can be secreted to the outside of the cells, killing *S. mutans* in the environment.

[http://parts.igem.org/Part:BBa\_K3378000](http://parts.igem.org/Part%3ABBa_K3378000)

# ClyR fused with PhoA signal peptide

PhoA fused ClyR can be secreted to the outside of the *E.coli*

[http://parts.igem.org/Part:BBa\_K3378001](http://parts.igem.org/Part%3ABBa_K3378001)

![](https://static.igem.org/mediawiki/2020/f/f3/T--HZAU-China--footercommonfinal.png)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:HZAU-China/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:HZAU-China/Results

# [![点击我返回主页](https://static.igem.org/mediawiki/2020/1/13/T--HZAU-China--logo2.png)](https://2020.igem.org/Team%3AHZAU-China)

- Project
  * [Description](https://2020.igem.org/Team%3AHZAU-China/Description)
  * [Design](https://2020.igem.org/Team%3AHZAU-China/Design)
  * [Implementation](https://2020.igem.org/Team%3AHZAU-China/Implementation)
  * [Proof\_Of\_Concept](https://2020.igem.org/Team%3AHZAU-China/Proof_Of_Concept)
- Wetlab
  * [Results](https://2020.igem.org/Team%3AHZAU-China/Results)
  * [Safety](https://2020.igem.org/Team%3AHZAU-China/Safety)
  * [Parts](https://2020.igem.org/Team%3AHZAU-China/Parts)
  * [Contribution](https://2020.igem.org/Team%3AHZAU-China/Contribution)
  * [Engineering](https://2020.igem.org/Team%3AHZAU-China/Engineering)
- Drylab
  * [Model](https://2020.igem.org/Team%3AHZAU-China/Model)
  * [Hardware](https://2020.igem.org/Team%3AHZAU-China/Hardware)
  * [Software](https://2020.igem.org/Team%3AHZAU-China/Software)
- Human Practice
  * [Human\_Practices](https://2020.igem.org/Team%3AHZAU-China/Human_Practices)
  * [Collaborations](https://2020.igem.org/Team%3AHZAU-China/Collaborations)
  * [Partnership](https://2020.igem.org/Team%3AHZAU-China/Partnership)
- About us
  * [Team](https://2020.igem.org/Team%3AHZAU-China/Team)
  * [Attributions](https://2020.igem.org/Team%3AHZAU-China/Attributions)

![](https://static.igem.org/mediawiki/2020/f/f9/T--HZAU-China--fairy.png)

# Results

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![](https://static.igem.org/mediawiki/2020/f/f3/T--HZAU-China--footercommonfinal.png)