iGEM 2012 — Team KAIT_Japan

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# Team:KAIT Japan/Notebook

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| | [![Kaitjapan.home.png](/wiki/images/8/87/Kaitjapan.home.png)](/Team%3AKAIT_Japan "Team:KAIT Japan") [Home](/Team%3AKAIT_Japan "Team:KAIT Japan") | | --- | | | [![Kaitjapan project.png](/wiki/images/3/3b/Kaitjapan_project.png)](/Team%3AKAIT_Japan/Project "Team:KAIT Japan/Project") [Project](/Team%3AKAIT_Japan/Project "Team:KAIT Japan/Project") | | --- | | | [![Kaitjapan parts.png](/wiki/images/c/c2/Kaitjapan_parts.png)](/Team%3AKAIT_Japan/Parts "Team:KAIT Japan/Parts") [Parts](/Team%3AKAIT_Japan/Parts "Team:KAIT Japan/Parts") | | --- | | | [![Kaitjapan protocol.png](/wiki/images/5/5a/Kaitjapan_protocol.png)](/Team%3AKAIT_Japan/Protocol "Team:KAIT Japan/Protocol") [Protocol](/Team%3AKAIT_Japan/Protocol "Team:KAIT Japan/Protocol") | | --- | | | [![Kaitjapan notebook.png](/wiki/images/a/ae/Kaitjapan_notebook.png)](/Team%3AKAIT_Japan/Notebook "Team:KAIT Japan/Notebook") **Notebook** | | --- | | | [![Kaitjapan results.png](/wiki/images/b/b8/Kaitjapan_results.png)](/Team%3AKAIT_Japan/Results "Team:KAIT Japan/Results") [Results](/Team%3AKAIT_Japan/Results "Team:KAIT Japan/Results") | | --- | | | [![Kaitjapan safety.png](/wiki/images/6/6d/Kaitjapan_safety.png)](/Team%3AKAIT_Japan/Safety "Team:KAIT Japan/Safety") [Safety](/Team%3AKAIT_Japan/Safety "Team:KAIT Japan/Safety") | | --- | | | [![Kaitjapan human practice.png](/wiki/images/8/8a/Kaitjapan_human_practice.png)](/Team%3AKAIT_Japan/Human_Practice "Team:KAIT Japan/Human Practice") [Human Practice](/Team%3AKAIT_Japan/Human_Practice "Team:KAIT Japan/Human Practice") | | --- | | | [![Kaitjapan team.png](/wiki/images/6/65/Kaitjapan_team.png)](/Team%3AKAIT_Japan/Team "Team:KAIT Japan/Team") [Team](/Team%3AKAIT_Japan/Team "Team:KAIT Japan/Team") | | --- | |

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| **Notebook** We made an experiment every day. It was process of trial and error. **Creating parts of Methylation and Azurin** **Date:8/9** PCR  **Date:8/10** Electrophoresis  **Date:8/13** Electrophoresis and The refinement of DNA,PCR  **Date:8/14** Transformation  **Date:8/16** Blue white selection and Miniprep,Colony PCR  **Date:8/17** Electrophoresis and Colony PCR  **Date:8/18** PCR and Electrophoresis  **Date:8/20** Extraction and the refinement of DNA  **Date:8/21** PCR and Electrophoresis,Transformation  **Date:8/22** Electrophoresis  **Date:8/23** PCR  **Date:8/25** Miniprep and Restriction enzyme processing  **Date:8/27** Electrophoresis  **Date:8/28** Electrophoresis and The refinement of DNA,PCR  **Date:8/29** Electrophoresis  **Date:8/30** Electrophoresis and The refinement of DNA  **Date:9/1** Extraction of DNA  **Date:9/2** Redesign of the primer  **Date:9/3** PCR and Restriction enzyme processing,Electrophoresis  **Date:9/5** Miniprep  **Date:9/6** Electrophoresis  **Date:9/10** Colony PCR  **Date:9/12** Miniprep  **Date:9/14** PCR  **Date:9/15** Restriction enzyme processing  **Date:9/16** PCR  **Date:9/17** PCR  **Date:9/19** PCR and Restriction enzyme processing  **Date:9/20** Ligation  **Date:9/22** Transformation  **Date:9/23** PCR and Electrophoresis  **Date:9/24** Ligation  **Date:9/25** Transformation  **Date:9/27** Miniprep and Electrophoresis **Creating parts of Tar** **Date:9/13** PCR and Colony PCR  **Date:9/14** Electrophoretic confirmation  **Date:9/15** Restriction enzyme processing  **Date:9/16** Electrophoresis of the restriction enzyme processing product and Restriction enzyme processing  **Date:9/17** Refinement of DNA and Ligation,Transformation  **Date:9/18** Making of a master plate and Preculture,Recultivation  **Date:9/19** Colony PCR and Electrophoresis  **Date:9/20** Ligation and Transformation  **Date:9/21** Inoculation  **Date:9/22** Making of a master plate and Colony PCR,Electrophoresis,Preculture  **Date:9/23** Electrophoretic confirmation and Electrophoresis of the PCR  **Date:9/24** Miniprep and Electrophoresis  **Date:9/25** PCR of the miniprep product and Electrophoresis **Creating parts of EnvZ** **Date:9/10** Design of the primer and PCR,Elecrtophoresis,Miniprep  **Date:9/13** PCR and Elecrtophoresis  **Date:9/18** Design of the primer  **Date:9/19** PCR and Elecrtophoresis  **Date:9/20** Restriction enzyme processing  **Date:9/21** Ligation and PCR,Elecrtophoresis  **Date:9/23** PCR  **Date:9/24** The refinement of DNA and Restriction enzyme processing,Ligation  **Date:9/25** Transformation  **Date:9/26** Liquid culture |

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