iGEM 2009 — Team Imperial_College_London

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# Team:Imperial College London/Notebook

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* **[Back to Hub](/Team%3AImperial_College_London/Wetlab "Team:Imperial College London/Wetlab")**
* **[Cloning Strategy](/Team%3AImperial_College_London/Wetlab/Cloning_Strategy "Team:Imperial College London/Wetlab/Cloning Strategy")**
* **[Protocols](/Team%3AImperial_College_London/Wetlab/Protocols "Team:Imperial College London/Wetlab/Protocols")**
* **[BioBricks](/Team%3AImperial_College_London/Wetlab/BioBricks "Team:Imperial College London/Wetlab/BioBricks")**
* **[Results](/Team%3AImperial_College_London/Wetlab/Results "Team:Imperial College London/Wetlab/Results")**
* ****Notebook****

# Notebook

Here is our daily notebook.

Please update all the experiments that you have performed, and any considerations along the way so that everyone can see the results and others can duplicate your experiments.

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| September | | | | | | |
| M | T | W | T | F | S | S |
|  | [http://2009.igem.org/Imperial\_College\_London/Notebook/1\_September\_2009 1] | [http://2009.igem.org/Imperial\_College\_London/Notebook/2\_September\_2009 2] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/3\_September\_2009&action=edit 3] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/4\_September\_2009&action=edit 4] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/5\_September\_2009&action=edit 5] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/6\_September\_2009&action=edit 6] |
| [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/7\_September\_2009&action=edit 7] | [http://2009.igem.org/Imperial\_College\_London/Notebook/8\_September\_2009 8] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/9\_September\_2009&action=edit 9] | [http://2009.igem.org/Imperial\_College\_London/Notebook/10\_September\_2009 10] | [http://2009.igem.org/Imperial\_College\_London/Notebook/11\_September\_2009 11] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/12\_September\_2009&action=edit 12] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/13\_September\_2009&action=edit 13] |
| [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/14\_September\_2009&action=edit 14] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/15\_September\_2009&action=edit 15] | [http://2009.igem.org/Imperial\_College\_London/Notebook/16\_September\_2009 16] | [http://2009.igem.org/Imperial\_College\_London/Notebook/17\_September\_2009 17] | [http://2009.igem.org/Imperial\_College\_London/Notebook/18\_September\_2009 18] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/19\_September\_2009&action=edit 19] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/20\_September\_2009&action=edit 20] |
| [http://2009.igem.org/Imperial\_College\_London/Notebook/21\_September\_2009 21] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/22\_September\_2009&action=edit 22] | [http://2009.igem.org/Imperial\_College\_London/Notebook/23\_September\_2009 23] | [http://2009.igem.org/Imperial\_College\_London/Notebook/24\_September\_2009 24] | [http://2009.igem.org/Imperial\_College\_London/Notebook/25\_September\_2009 25] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/26\_September\_2009&action=edit 26] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/27\_September\_2009&action=edit 27] |
| [http://2009.igem.org/Imperial\_College\_London/Notebook/28\_September\_2009 28] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/29\_September\_2009&action=edit 29] | [http://2009.igem.org/Imperial\_College\_London/Notebook/30\_September\_2009 30] |

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| October | | | | | | |
| M | T | W | T | F | S | S |
|  |  |  | [http://2009.igem.org/Imperial\_College\_London/Notebook/1\_October\_2009 1] | [http://2009.igem.org/Imperial\_College\_London/Notebook/2\_October\_2009 2] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/3\_October\_2009&action=edit 3] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/4\_October\_2009&action=edit 4] |
| [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/5\_October\_2009&action=edit 5] | [http://2009.igem.org/Imperial\_College\_London/Notebook/6\_October\_2009 6] | [http://2009.igem.org/Imperial\_College\_London/Notebook/7\_October\_2009 7] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/8\_October\_2009&action=edit 8] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/9\_October\_2009&action=edit 9] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/10\_October\_2009&action=edit 10] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/11\_October\_2009&action=edit 11] |
| [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/12\_October\_2009&action=edit 12] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/13\_October\_2009&action=edit 13] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/14\_October\_2009&action=edit 14] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/15\_October\_2009&action=edit 15] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/16\_October\_2009&action=edit 16] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/17\_October\_2009&action=edit 17] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/18\_October\_2009&action=edit 18] |
| [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/19\_October\_2009&action=edit 19] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/20\_October\_2009&action=edit 20] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/21\_October\_2009&action=edit 21] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/22\_October\_2009&action=edit 22] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/23\_October\_2009&action=edit 23] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/24\_October\_2009&action=edit 24] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/25\_October\_2009&action=edit 25] |
| [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/26\_October\_2009&action=edit 26] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/27\_October\_2009&action=edit 27] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/28\_October\_2009&action=edit 28] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/29\_October\_2009&action=edit 29] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/30\_October\_2009&action=edit 30] | [http://2009.igem.org/wiki/index.php?title=Imperial\_College\_London/Notebook/31\_October\_2009&action=edit 31] |

# BioBrick Progress

The table below shows the progress/status of each BioBrick part/construct:

|  |  |  |  |  |  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- |
| | Key | | --- | | Submitted | | Sequenced | | Midi-prepped | | Fully verified | | Partially verified | | Transformed | | Ligation pending/Failed | | SLIC | | Site-directed mutagenesis | | Awaiting Mr.Gene | |

| Part Number | Part Name | Status |
| --- | --- | --- |
| BBa\_K200000 | RcsB | Sequenced from mini-prep/SDM on midi-prep |
| BBa\_K200001 | Dam methylase | Mini-prepped |
| BBa\_K200002 | ygiV (B3023) | Mini-prepped |
| BBa\_K200003 | Waal Ligase | Mini-prepped |
| BBa\_K200005 | OtsA | Sequenced from midi-prep |
| BBa\_K200006 | OtsB | Sequenced from midi-prep |
| BBa\_K200007 | Cellulase | Transformed |
| BBa\_K200008 | Phenylalanine hydroxylase | Site-directed mutagenesis/mini-preps |
| BBa\_K200009 | DpnII restriction enzyme | Awaiting Mr. Gene |
| BBa\_K200009 | TaqI restriction enzyme (RBS+TaqI+TT) | Transformed |
| BBa\_K200011 | Lambda cI repressor | Awaiting Mr. Gene |
| BBa\_K200012 | Lambda promoter | Sequenced from mini-prep |
| BBa\_ | pLacI | Sequenced from mini-prep |
| BBa\_ | pCstA | Sequenced from mini-prep |
| BBa\_ | pCstA+RBS+GFP+TT | Transformed |
| BBa\_ | pCstA+RBS+GFP+TT+pLacI+RBS+RFP+TT | Transformed |
| BBa\_ | RBS+OtsB | Mini-prepped |
| BBa\_ | pCstA+RBS | Mini-prepped |
| BBa\_ | LacI+RBS | Mini-prepped |

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