iGEM 2020 — Team XHD-Wuhan-China

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# Team:XHD-Wuhan-China/Notebook

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Notebook

[Notebook1](https://static.igem.org/mediawiki/2020/b/b8/T--XHD-Wuhan-China--NOTEBOOK.pdf)
[Notebook2](https://static.igem.org/mediawiki/2020/c/c4/T--XHD-Wuhan-China--NOTEBOOK2.pdf)

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Copyright ©2020 iGEM XHD-Wuhan-China All rights reserved

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# Team:XHD-Wuhan-China/Engineering

![](https://static.igem.org/mediawiki/2020/4/41/T--XHD-Wuhan-China--TeamLogo.png)

Engineering

To achieve the purpose of further dissolving phosphate, we designed
the Phosphate dissolving Plus system.

Phosphate dissolving Plus system

We design an enhanced Phosphate dissolving system (Phosphate dissolving
Plus system) which has two plasmids: gcd-pRADK, gabY-pRADK. And we designed gabY gene
circuit PGroES-RBS-gabY (Figure 1). In order to realize the
function of gabY gene circuit, we constructed the following plasmid
gabY-pRADK (Figure 2).

![](https://static.igem.org/mediawiki/2020/7/7f/T--XHD-Wuhan-China--Engineering-figure1.png)

Figure 1. Constitution of PGroES-RBS-gabY gene circuits.

![](https://static.igem.org/mediawiki/2020/9/96/T--XHD-Wuhan-China--Design-figure4.png)

Figure 2. Design of Phosphate dissolving Plus plasmid (gabY-pRADK).

Usage and Biology

We verified the length of the recombinant plasmid to ensure the
success of the recombinant plasmid through enzyme digestion
(Figure 3). Theoretically, after transforming gabY-pRADK to DR
containing gcd-pRADK, the effective concentration of the holoenzyme
formed by GDH and PQQ coenzymes will increase, and the catalytic
efficiency will be higher. We cultured DR containing gcd-pRADK,
gabY-pRADK; DR containing gcd-pRADK; and wild-type DR in TGY liquid
medium, and measured pH every 1 hour. The results are shown in the
(Figure 4). In addition, DR containing gcd-pRADK, gabY-pRADK; DR
containing gcd-pRADK; and wild-type DR were cultured in PKO solid
medium, and the results of the phosphate ring were observed, as shown
in the (Figure 5).

![](https://static.igem.org/mediawiki/2020/4/42/T--XHD-Wuhan-China--result-figuregabYEl.png)

Figure 3. ElectropHoresis of plasmid gabY-pRADK with enzyme digestions.

![](https://static.igem.org/mediawiki/2020/6/6e/T--XHD-Wuhan-China--result-figure11.png)

Figure 4. Changes in pH value of TGY liquid medium culturing DR R1, DR containing gcd-pRADK and DR containing pRADK2 respectively.

![](https://static.igem.org/mediawiki/2020/e/e2/T--XHD-Wuhan-China--result-figure12gcd-gabY.jpeg)

Figure 5. Phosphate ring in PKO solid medium culturing DR R1, DR containing gcd-pRADK and DR containing pRADK2 respectively. Left: DR R1; Middle: DR containing gcd-pRADK; Right: DR containing pRADK2.

Conclusion

We constructed a phosphate dissolving Plus system in Deinococcus
radiodurans and successfully verified the reliability of the phosphate dissolving Plus system. At the same time, we
also compare the differences of different systems and those can improve the
Martian soil in a targeted manner. Our project can provide new
solutions to improve the Martian soil and increase the possibility of
growing crops on Mars.

![](https://static.igem.org/mediawiki/2020/e/e7/T--XHD-Wuhan-China--CommonImg1.jpg)
![](https://static.igem.org/mediawiki/2020/a/aa/T--XHD-Wuhan-China--CommonImg2.jpg)

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Copyright ©2020 iGEM XHD-Wuhan-China All rights reserved

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# Team:XHD-Wuhan-China/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:XHD-Wuhan-China/Results

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)