iGEM 2017 — Team XMU-China

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:XMU-China/Notebook

2017.igem.org/Team:XMU-China/Notebook

* [![](https://static.igem.org/mediawiki/2017/9/9a/T--XMU-China--iconhome.png)](https://2017.igem.org/Team%3AXMU-China)
* PROJECT
  + [Description](https://2017.igem.org/Team%3AXMU-China/Description)
  + [Design](https://2017.igem.org/Team%3AXMU-China/Design)
  + [Demonstrate](https://2017.igem.org/Team%3AXMU-China/Demonstrate)
  + [Results](https://2017.igem.org/Team%3AXMU-China/Results)
  + [Applied Design](https://2017.igem.org/Team%3AXMU-China/Applied_Design)
  + [Parts](https://2017.igem.org/Team%3AXMU-China/Parts)
* NOTEBOOK
  + [Notebook](https://2017.igem.org/Team%3AXMU-China/Notebook)
  + [Experiments](https://2017.igem.org/Team%3AXMU-China/Experiments)
  + [Engineering](https://2017.igem.org/Team%3AXMU-China/Engineering)
* HARDWARE
  + [Overview](https://2017.igem.org/Team%3AXMU-China/Hardware)
  + [Freeze-Dry](https://2017.igem.org/Team%3AXMU-China/Freeze-Dry)
  + [East-Wind](https://2017.igem.org/Team%3AXMU-China/East-Wind)
  + [Accessories](https://2017.igem.org/Team%3AXMU-China/Accessories)
  + [More Chips](https://2017.igem.org/Team%3AXMU-China/More_Chips)
* MODEL
  + [Overview](https://2017.igem.org/Team%3AXMU-China/Model)
  + [Modeling](https://2017.igem.org/Team%3AXMU-China/Modeling)
* HUMAN PRACTICES
  + [Overview](https://2017.igem.org/Team%3AXMU-China/Human_Practices)
  + [Silver](https://2017.igem.org/Team%3AXMU-China/HP/Silver)
  + [Gold](https://2017.igem.org/Team%3AXMU-China/HP/Gold_Integrated)
  + [Engagement](https://2017.igem.org/Team%3AXMU-China/Engagement)
  + [Entrepreneurship](https://2017.igem.org/Team%3AXMU-China/Entrepreneurship)
  + [Collaborations](https://2017.igem.org/Team%3AXMU-China/Collaborations)
* OTHER WORK
  + [Interlab](https://2017.igem.org/Team%3AXMU-China/InterLab)
  + [Contribution](https://2017.igem.org/Team%3AXMU-China/Contribution)
  + [Improve](https://2017.igem.org/Team%3AXMU-China/Improve)
  + [Measurement](https://2017.igem.org/Team%3AXMU-China/Measurement)
  + [Safety](https://2017.igem.org/Team%3AXMU-China/Safety)
* TEAM
  + [Team](https://2017.igem.org/Team%3AXMU-China/Team)
  + [Attributions](https://2017.igem.org/Team%3AXMU-China/Attributions)
  + [Judging](https://2017.igem.org/Team%3AXMU-China/Judging)

![](https://static.igem.org/mediawiki/2017/2/2a/T--XMU-China--notebookbg.png)

* March
  March

  - Our team was formally founded.
  - All student members learned the fundamental knowledge of molecular cloning and iGEM.
  - All student members looked through the previous excellent projects and learned the basic notion of synthetic biology and iGEM.
* April
  April

  - We divided into three groups and started to brainstorm separately (and we reported each’s progress every weekend).
  - Our team was formally accepted by iGEM.
* May
  May

  - Our formal project was confirmed.
  - The pre-experiment plan was confirmed.
  - The division of labor was confirmed.
* Week 1-2
  June

  - All student members learned laboratory fundamental qualities and received molecular cloning operation training.
* Week 3

  - 2017 DNA Distribution Kit Plates were received.
  - We transformed all the biobricks we needed.
* Week 4

  - [BBa\_K2310001](http://parts.igem.org/Part%3ABBa_K2310001) constructed.
  - [BBa\_K2310002](http://parts.igem.org/Part%3ABBa_K2310002), [BBa\_K2310003](http://parts.igem.org/Part%3ABBa_K2310003) and [BBa\_K2310004](http://parts.igem.org/Part%3ABBa_K2310004) constructed.

  ![](https://static.igem.org/mediawiki/2017/6/6f/T--XMU-China--notebookimg1.jpeg)
* Week 1
  July

  - [BBa\_K2310005](http://parts.igem.org/Part%3ABBa_K2310005) constructed

  ![](https://static.igem.org/mediawiki/2017/c/c1/T--XMU-China--notebookimg2.png)
* Week 3

  - [BBa\_K2310101](http://parts.igem.org/Part%3ABBa_K2310101) constructed

  ![](https://static.igem.org/mediawiki/2017/2/20/T--XMU-China--notebookimg3.png)
* Week 4

  - [BBa\_K2310000](http://parts.igem.org/Part%3ABBa_K2310000) constructed
  - [BBa\_K2310103](http://parts.igem.org/Part%3ABBa_K2310103) constructed

  ![](https://static.igem.org/mediawiki/2017/1/12/T--XMU-China--notebookimg4.png)

  - The first try of freeze dried bacteria

  ![](https://static.igem.org/mediawiki/2017/a/a2/T--XMU-China--notebookimg5.png)
* Week 1
  August

  - [BBa\_K2310006](http://parts.igem.org/Part%3A%3EBBa_K2310006) constructed

  ![](https://static.igem.org/mediawiki/2017/e/e2/T--XMU-China--notebookimg6.png)

  - [BBa\_K2310007](http://parts.igem.org/Part%3ABBa_K2310007) constructed

  ![](https://static.igem.org/mediawiki/2017/3/3e/T--XMU-China--notebookimg7.png)

  - [BBa\_K2310008](http://parts.igem.org/Part%3ABBa_K2310008) constructed

  ![](https://static.igem.org/mediawiki/2017/5/50/T--XMU-China--notebookimg8.png)

  - [BBa\_K2310100](http://parts.igem.org/Part%3ABBa_K2310100) tested

  ![](https://static.igem.org/mediawiki/2017/1/1a/T--XMU-China--notebookimg9.jpeg)
* Week 2

  - [BBa\_K2310009](http://parts.igem.org/Part%3ABBa_K2310009) constructed

  ![](https://static.igem.org/mediawiki/2017/c/cd/T--XMU-China--notebookimg10.png)

  - [BBa\_K2310011](http://parts.igem.org/Part%3ABBa_K2310011) constructed

  ![](https://static.igem.org/mediawiki/2017/5/50/T--XMU-China--notebookimg11.png)
* Week 3

  - [BBa\_K2310105](http://parts.igem.org/Part%3ABBa_K2310105) constructed

  ![](https://static.igem.org/mediawiki/2017/7/7a/T--XMU-China--notebookimg12.png)

  - [BBa\_K2310106](http://parts.igem.org/Part%3ABBa_K2310106) constructed

  ![](https://static.igem.org/mediawiki/2017/c/c4/T--XMU-China--notebookimg13.png)

  - [BBa\_K2310102](http://parts.igem.org/Part%3ABBa_K2310102) and [BBa\_K2310108](http://parts.igem.org/Part%3ABBa_K2310108) constructed
* Week 4

  - [BBa\_K2310107](http://parts.igem.org/Part%3ABBa_K2310107) constructed
* Week 1
  September

  - Micro-fluid chip tested

  ![](https://static.igem.org/mediawiki/2017/9/96/T--XMU-China--notebookimg14.png)
* Week 2

  - [BBa\_K2310012](http://parts.igem.org/Part%3ABBa_K2310012) constructed

  ![](https://static.igem.org/mediawiki/2017/6/67/T--XMU-China--notebookimg15.png)
* Week 3

  - PCR amplified LPP, INP-SA and iLOV gene fragment and obtained the plasmid

  ![](https://static.igem.org/mediawiki/2017/3/37/T--XMU-China--notebookimg16.png)
* Week 4

  - Freeze dried bacteria experiments to explore some experimental conditions

  ![](https://static.igem.org/mediawiki/2017/2/2c/T--XMU-China--notebookimg17.png)
* Week 1
  October

  - The first round of the characteristic experiment
  - The luxAB chemiluminescence experiment to explore some experimental conditions

  ![](https://static.igem.org/mediawiki/2017/4/4d/T--XMU-China--notebookimg18.png)

  ![](https://static.igem.org/mediawiki/2017/a/a3/T--XMU-China--notebookimg19.png)
* Week 2

  - The second round of the characteristic experiment
* Week 3

  - Hardware tested

Xiamen University, Fujian, China
No. 422, Siming South Road, Xiamen, Fujian, P. R. China 361005