iGEM 2018 — Team ZJU-China

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# Team:ZJU-China/Notebook

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[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3AZJU-China)
[HOME](https://2018.igem.org/Team%3AZJU-China)

TEAM

[Team Members](https://2018.igem.org/Team%3AZJU-China/Team)
[Collaborations](https://2018.igem.org/Team%3AZJU-China/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AZJU-China/Description)
[Design](https://2018.igem.org/Team%3AZJU-China/Design)
[Experiments](https://2018.igem.org/Team%3AZJU-China/Experiments)
[Notebook](https://2018.igem.org/Team%3AZJU-China/Notebook)
[InterLab](https://2018.igem.org/Team%3AZJU-China/InterLab)
[Model](https://2018.igem.org/Team%3AZJU-China/Model)
[Results](https://2018.igem.org/Team%3AZJU-China/Results)
[Demonstrate](https://2018.igem.org/Team%3AZJU-China/Demonstrate)
[Improve](https://2018.igem.org/Team%3AZJU-China/Improve)
[Attributions](https://2018.igem.org/Team%3AZJU-China/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AZJU-China/Parts)
[Basic Parts](https://2018.igem.org/Team%3AZJU-China/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AZJU-China/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AZJU-China/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AZJU-China/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AZJU-China/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AZJU-China/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AZJU-China/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AZJU-China/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AZJU-China/Hardware)
[Measurement](https://2018.igem.org/Team%3AZJU-China/Measurement)
[Model](https://2018.igem.org/Team%3AZJU-China/Model)
[Plant](https://2018.igem.org/Team%3AZJU-China/Plant)
[Software](https://2018.igem.org/Team%3AZJU-China/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=ZJU-China)

Model

menu

* Project
  + [Demonstrate](https://2018.igem.org/Team%3AZJU-China/Demonstrate)
  + [Enzyme Scaffold](https://2018.igem.org/Team%3AZJU-China/EnzymeScaffold)
  + [Logic Gate](https://2018.igem.org/Team%3AZJU-China/LogicGate)
  + [Matrix](https://2018.igem.org/Team%3AZJU-China/Matrix)
  + [Electrodes](https://2018.igem.org/Team%3AZJU-China/Electrodes)
* Applied Design
  + [Product Design](https://2018.igem.org/Team%3AZJU-China/Applied_Design)
  + [Hardware](https://2018.igem.org/Team%3AZJU-China/Hardware)
* [Model](https://2018.igem.org/Team%3AZJU-China/Model)
* Human Practice
  + [Silver&Gold](https://2018.igem.org/Team%3AZJU-China/Human_Practices)
  + [Public Engagement](https://2018.igem.org/Team%3AZJU-China/Public_Engagement)
  + [Collaborations](https://2018.igem.org/Team%3AZJU-China/Collaborations)
* Parts
  + [All parts](https://2018.igem.org/Team%3AZJU-China/AllParts)
  + [Basic Parts](https://2018.igem.org/Team%3AZJU-China/BasicPart)
  + [Composite Parts](https://2018.igem.org/Team%3AZJU-China/CompositePart)
  + [Collection](https://2018.igem.org/Team%3AZJU-China/Collection)
  + [Improved Parts](https://2018.igem.org/Team%3AZJU-China/Improve)
* Team
  + + [Members](https://2018.igem.org/Team%3AZJU-China/Team)
    + [Attributions](https://2018.igem.org/Team%3AZJU-China/Attributions)
    + Notebook
      - [Lab book](https://2018.igem.org/Team%3AZJU-China/Notebook)
      - [Protocols](https://2018.igem.org/Team%3AZJU-China/Protocols)
* [InterLab](https://2018.igem.org/Team%3AZJU-China/InterLab)
* [Safety](https://2018.igem.org/Team%3AZJU-China/Safety)

NOTEBOOK

---

Lab Book

1. [End Of Winter](#0)
2. [Mar.8 - Jun.2](#0)
3. [Jun. 15](#0)
4. [Jun. 23](#0)
5. [Jun. 25](#0)
6. [Jul. 13](#0)
7. [Jul. 25](#0)
8. [Jul. 29](#0)
9. [Aug. 6](#0)
10. [Aug. 14](#0)
11. [Aug. 18](#0)
12. [Aug. 25](#0)
13. [Aug. 26](#0)
14. [Sep. 3](#0)
15. [Sep. 25](#0)
16. [Oct. 1 - 15](#0)

* [Prev](#0)
* [Next](#0)

1. *End of Winter*

   After the end of the winter selection, we formed a new team - ZJU-China 2018. It is the beginning of the story.

   ![](https://static.igem.org/mediawiki/2018/5/5e/T--ZJU-China--notebook1.jpg)
2. *March 8 - June 2*

   At the group meeting, everyone had brainstormed many times. This is a rather long process, many ideas were proposed and rejected, and finally, we reached a consensus - to create a cool artificial multi-enzyme complex

   At the group meeting, everyone had brainstormed many times. This is a rather long process, many ideas were proposed and rejected, and finally, we reached a consensus - to create a cool artificial multi-enzyme complex!

   ![](https://static.igem.org/mediawiki/2018/7/7f/T--ZJU-China--notebook2.jpg)
3. *June 15*

   We went out to play and know each other better!

   ![](https://static.igem.org/mediawiki/2018/8/84/T--ZJU-China--notebook3.jpg)
4. *June 23*

   We officially confirmed the project as a protein scaffold and started the experiment.

   ![](https://static.igem.org/mediawiki/2018/d/dc/T--ZJU-China--notebook4.jpg)
5. *June 25*

   We divided our project into three parts. 1 - Curli expression. 2 - Enzyme construction 3 - Electrode modification. We prepared essential material and protocols for the the most important battlefield - Summer!

   ![](https://static.igem.org/mediawiki/2018/e/e2/T--ZJU-China--notebook5.jpg)
6. *July 13*

   The summer vacation had begun. The csgA protein has been successfully expressed. However, the construction of csgA KO strains met some difficulties. Despite this, the staged results still make us very happy!

   ![](https://static.igem.org/mediawiki/2018/a/a0/T--ZJU-China--notebook6.jpg)
7. *July 25*

   We have successfully constructed recombination strains with exogenous protein. Since the expression of eukaryotic proteins in prokaryotic systems is indeed a challenge, we have many failures. However, after many attempts, we successfully completed the expected goal!

   ![](https://static.igem.org/mediawiki/2018/8/81/T--ZJU-China--notebook7.jpg)
8. *July 29*

   We had a pleasant meeting with ACON Bio. After showing them the project, they feel that our project has great possibilities, but it needs to be improved in some practical aspects.

   ![](https://static.igem.org/mediawiki/2018/1/12/T--ZJU-China--notebook8.jpg)
9. ##

   *August 6*

   We went to the Zhejiang Science and Technology Museum and hoped to promote our own projects. In a cheerful atmosphere, we not only introduced them to the basic knowledge of synthetic biology, but also introduced some of our projects. To our surprise, the children have a very strong interest in our projects. A child even pointed out a problem that we ourselves are confused! What a surprising experience.

   ![](https://static.igem.org/mediawiki/2018/d/d2/T--ZJU-China--notebook9.jpg)
10. *August 14*

    We have learned the electrode technology and decided to make two products - one is basic, using electrodes as well as electrochemical workstations, the other is smaller, and can even in a pocket.

    Work on 2D printing is also progressing well. We made a practical 2D printer and prepared to use it to accurately print the enzyme onto the electrode.

    ![](https://static.igem.org/mediawiki/2018/1/1e/T--ZJU-China--notebook10.jpg)
11. *August 18*

    We had sought help from Shanghai Tech University and gotten a csgA-KO strain. With this bacterium, our project has taken a big step forward. By testing the KO strain, we verified the function of Curli and prepared to fix it to the electrode in the next step.

    ![](https://static.igem.org/mediawiki/2018/2/22/T--ZJU-China--notebook11.jpg)
12. *August 25*

    We went to CCIC. This is the Asian Forum on Synthetic Biology, and we showcased our projects. Other teams in china gave us many useful suggestions and had a lot of communication with us. Through CCIC, we knew everyone's progress and also clarified the subsequent tasks.

    ![](https://static.igem.org/mediawiki/2018/5/56/T--ZJU-China--notebook12.jpg)
13. *August 26*

    We have consulted some question about safety with Joichim messing，he gave us a lot of practical advises. Thanks so much for him!

    ![](https://static.igem.org/mediawiki/2018/9/90/T--ZJU-China--notebook13.jpg)
14. *September 3*

    We basically completed the production of the first generation of products. The results given by the electrochemical workstation are very satisfactory. However, its large size limits the practicality of the product. At the same time, the development of the second generation of smaller and lighter products is nearing completion. We are going to get the final result!

    ![](https://static.igem.org/mediawiki/2018/1/1e/T--ZJU-China--notebook14.jpg)
15. *September 25*

    We finally finished the final product! A beautiful interdigital electrode successfully distinguishes between the patient and the normal person. Although its stability needs to be further improved, this achievement is very exciting!

    ![](https://static.igem.org/mediawiki/2018/c/cd/T--ZJU-China--notebook15.jpg)
16. *October 1 – 15*

    After completing the final optimization of the product, we started writing wikis. Writing a wiki every day keeps doctor away.

    ![](https://static.igem.org/mediawiki/2018/b/b9/T--ZJU-China--notebook16.jpg)

Our Sponsors

![](https://static.igem.org/mediawiki/2018/6/68/T--ZJU-China--home--biolab.png)
![](https://static.igem.org/mediawiki/2018/f/f1/T--ZJU-China--genscript.png)
![](https://static.igem.org/mediawiki/2018/b/b2/T--ZJU-China--home--undergraduate.png)
![](https://static.igem.org/mediawiki/2018/5/5f/T--ZJU-China--foundation.png)
![](https://static.igem.org/mediawiki/2018/7/7f/T--ZJU-China--hospital.png)


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:ZJU-China/Experiments

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[HOME](https://2018.igem.org/Team%3AZJU-China)

TEAM

[Team Members](https://2018.igem.org/Team%3AZJU-China/Team)
[Collaborations](https://2018.igem.org/Team%3AZJU-China/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AZJU-China/Description)
[Design](https://2018.igem.org/Team%3AZJU-China/Design)
[Experiments](https://2018.igem.org/Team%3AZJU-China/Experiments)
[Notebook](https://2018.igem.org/Team%3AZJU-China/Notebook)
[InterLab](https://2018.igem.org/Team%3AZJU-China/InterLab)
[Model](https://2018.igem.org/Team%3AZJU-China/Model)
[Results](https://2018.igem.org/Team%3AZJU-China/Results)
[Demonstrate](https://2018.igem.org/Team%3AZJU-China/Demonstrate)
[Improve](https://2018.igem.org/Team%3AZJU-China/Improve)
[Attributions](https://2018.igem.org/Team%3AZJU-China/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AZJU-China/Parts)
[Basic Parts](https://2018.igem.org/Team%3AZJU-China/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AZJU-China/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AZJU-China/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AZJU-China/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AZJU-China/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AZJU-China/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AZJU-China/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AZJU-China/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AZJU-China/Hardware)
[Measurement](https://2018.igem.org/Team%3AZJU-China/Measurement)
[Model](https://2018.igem.org/Team%3AZJU-China/Model)
[Plant](https://2018.igem.org/Team%3AZJU-China/Plant)
[Software](https://2018.igem.org/Team%3AZJU-China/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=ZJU-China)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:ZJU-China/Results

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[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3AZJU-China)
[HOME](https://2018.igem.org/Team%3AZJU-China)

TEAM

[Team Members](https://2018.igem.org/Team%3AZJU-China/Team)
[Collaborations](https://2018.igem.org/Team%3AZJU-China/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AZJU-China/Description)
[Design](https://2018.igem.org/Team%3AZJU-China/Design)
[Experiments](https://2018.igem.org/Team%3AZJU-China/Experiments)
[Notebook](https://2018.igem.org/Team%3AZJU-China/Notebook)
[InterLab](https://2018.igem.org/Team%3AZJU-China/InterLab)
[Model](https://2018.igem.org/Team%3AZJU-China/Model)
[Results](https://2018.igem.org/Team%3AZJU-China/Results)
[Demonstrate](https://2018.igem.org/Team%3AZJU-China/Demonstrate)
[Improve](https://2018.igem.org/Team%3AZJU-China/Improve)
[Attributions](https://2018.igem.org/Team%3AZJU-China/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AZJU-China/Parts)
[Basic Parts](https://2018.igem.org/Team%3AZJU-China/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AZJU-China/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AZJU-China/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AZJU-China/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AZJU-China/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AZJU-China/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AZJU-China/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AZJU-China/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AZJU-China/Hardware)
[Measurement](https://2018.igem.org/Team%3AZJU-China/Measurement)
[Model](https://2018.igem.org/Team%3AZJU-China/Model)
[Plant](https://2018.igem.org/Team%3AZJU-China/Plant)
[Software](https://2018.igem.org/Team%3AZJU-China/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=ZJU-China)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)