iGEM 2008 — Team MIT

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# Team:MIT/Notebook

### From 2008.igem.org

| [Home](/Team%3AMIT "Team:MIT") | [The Team](/Team%3AMIT/Team "Team:MIT/Team") | [The Project](/Team%3AMIT/Project "Team:MIT/Project") | [Experiments](/Team%3AMIT/Experiments "Team:MIT/Experiments") | [Parts Submitted to the Registry](/Team%3AMIT/Parts "Team:MIT/Parts") | [Results](/Team%3AMIT/Modeling "Team:MIT/Modeling") | **Notebook** |
| --- | --- | --- | --- | --- | --- | --- |

## Procedural Daily Notebook

* Click on any day below to see what wet-lab procedures were conducted.

## Organizational Daily Notebook

* Please see our [daily notebook](/Team%3AMIT/Organizational_Daily_Notebook "Team:MIT/Organizational Daily Notebook") for a detailed daily summary of our daily meetings, brainstorming, discussions, and progress.

## Information

* [Primers/Plasmids/PCR sequences](/Team%3AMIT/sequences "Team:MIT/sequences")
* [Lactobacillus delbrueckii subsp. bulgaricus Information & Protocols](/Team%3AMIT/Lactobacillus "Team:MIT/Lactobacillus")
* [Vocabulary List](/Team%3AMIT/Vocabulary_List "Team:MIT/Vocabulary List")

## Protocols

* [http://openwetware.org/wiki/MIT\_iGEM\_T4\_and\_Quick\_Ligation DNA Ligations]
* [http://openwetware.org/wiki/MIT\_iGEM\_Top10\_ChemComp\_Ecoli\_transformation\_protocol#Regular\_Transformation\_protocol\_for\_Top10\_cells Top10 chem competent cell transformation]
* [http://openwetware.org/wiki/Knight:Electroporation BLR electrocompetent cell transformation]
* [http://web.mit.edu/biopolymers/www/DNA.html DNA sequencing]
* [http://openwetware.org/wiki/Endy\_pcr PCR]
* [http://openwetware.org/wiki/MIT\_iGEM\_Agarose\_Gels Running DNA Gels]
* [http://openwetware.org/wiki/MIT\_iGEM\_Restriction\_Digests Restriction Digests]
* [http://openwetware.org/wiki/Endy:Preparing\_Antibiotic\_Stocks Preparing Antibiotic Stocks]
* [Protein Purification](/Team%3AMIT/Protein_Purification "Team:MIT/Protein Purification")
* [Novel Tooth Binding Assay](/Team%3AMIT/Tooth_binding_assay_protocol "Team:MIT/Tooth binding assay protocol")
* [Lactococcus lactis Transformation Protocol](/Team%3AMIT/Transforming_Lactococcus_lactis "Team:MIT/Transforming Lactococcus lactis")
* [Colony PCR](/Team%3AMIT/Colony_PCR "Team:MIT/Colony PCR")

## Data

* [Toothbinding assay](/Team%3AMIT/Notebook/Data/toothbinding "Team:MIT/Notebook/Data/toothbinding")

## Useful Links

* [http://www.biology.utah.edu/jorgensen/wayned/ape/ DNA Plasmid Editor]
* [http://slam.bs.jhmi.edu/gd/ Peptide Sequence Manipulations]
* Primer Analyzer Tool (check temperature & GC content) [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | One] [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | Two]
* [PMID 9920267](https://www.ncbi.nlm.nih.gov/pubmed/9920267?dopt=Abstract)
   A synthetic peptide adhesion epitope as a novel antimicrobial agent.
* [PMID 9062560](https://www.ncbi.nlm.nih.gov/pubmed/9062560?dopt=Abstract)
   Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.
* [PMID 4055036](https://www.ncbi.nlm.nih.gov/pubmed/4055036?dopt=Abstract)
   Albumin as a blocking agent in studies of streptococcal adsorption to experimental salivary pellicles.
* [PMID 12788739](https://www.ncbi.nlm.nih.gov/pubmed/12788739?dopt=Abstract)
   Determination of the domain of the Lactobacillus delbrueckii subsp. bulgaricus cell surface proteinase PrtB involved in attachment to the cell wall after heterologous expression of the prtB gene in Lactococcus lactis. (a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).
* [PMID 11772607](https://www.ncbi.nlm.nih.gov/pubmed/11772607?dopt=Abstract)
   Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.
* High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol [http://www.biomedcentral.com/1472-6750/7/15]

| [Home](/Team%3AMIT "Team:MIT") | [The Team](/Team%3AMIT/Team "Team:MIT/Team") | [The Project](/Team%3AMIT/Project "Team:MIT/Project") | [Experiments](/Team%3AMIT/Experiments "Team:MIT/Experiments") | [Parts Submitted to the Registry](/Team%3AMIT/Parts "Team:MIT/Parts") | [Modeling](/Team%3AMIT/Modeling "Team:MIT/Modeling") | **Notebook** |
| --- | --- | --- | --- | --- | --- | --- |

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