iGEM 2013 — Team HokkaidoU_Japan

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# Team:HokkaidoU Japan/Notebook

### From 2013.igem.org

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# HOKKAIDO U](https://2013.igem.org/Team%3AHokkaidoU_Japan)

* [Top](https://2013.igem.org/Team%3AHokkaidoU_Japan)
* [Maestro E. coli](https://2013.igem.org/Team%3AHokkaidoU_Japan/Overview)
  + [Overview](https://2013.igem.org/Team%3AHokkaidoU_Japan/Overview)
    - [Project Description](https://2013.igem.org/Team%3AHokkaidoU_Japan/Overview)
    - [Background](https://2013.igem.org/Team%3AHokkaidoU_Japan/Overview/Background)
    - [Achievements](https://2013.igem.org/Team%3AHokkaidoU_Japan/Overview/Achievements)
  + [Shuffling Kit](https://2013.igem.org/Team%3AHokkaidoU_Japan/Shuffling_Kit)
    - [Overview](https://2013.igem.org/Team%3AHokkaidoU_Japan/Shuffling_Kit)
    - [How To Use](https://2013.igem.org/Team%3AHokkaidoU_Japan/Shuffling_Kit/How_To_Use)
    - [Primer Designer](https://2013.igem.org/Team%3AHokkaidoU_Japan/Shuffling_Kit/Primer_Designer)
    - [Examples](https://2013.igem.org/Team%3AHokkaidoU_Japan/Shuffling_Kit/Examples)
    - [Future Work](https://2013.igem.org/Team%3AHokkaidoU_Japan/Shuffling_Kit/Future_Work)
  + [Promoter](https://2013.igem.org/Team%3AHokkaidoU_Japan/Promoter)
    - [Overview](https://2013.igem.org/Team%3AHokkaidoU_Japan/Promoter)
    - [Methods](https://2013.igem.org/Team%3AHokkaidoU_Japan/Promoter/Methods)
    - [Modeling](https://2013.igem.org/Team%3AHokkaidoU_Japan/Promoter/Modeling)
    - [Results](https://2013.igem.org/Team%3AHokkaidoU_Japan/Promoter/Results)
    - [Conclusion](https://2013.igem.org/Team%3AHokkaidoU_Japan/Promoter/Conclusion)
  + [RBS](https://2013.igem.org/Team%3AHokkaidoU_Japan/RBS)
    - [Overview](https://2013.igem.org/Team%3AHokkaidoU_Japan/RBS)
    - [Methods](https://2013.igem.org/Team%3AHokkaidoU_Japan/RBS/Methods)
    - [Results](https://2013.igem.org/Team%3AHokkaidoU_Japan/RBS/Results)
    - [Conclusion](https://2013.igem.org/Team%3AHokkaidoU_Japan/RBS/Conclusion)
  + [Extra Materials](https://2013.igem.org/Team%3AHokkaidoU_Japan/Materials)
    - [Parts](https://2013.igem.org/Team%3AHokkaidoU_Japan/Parts)
    - [Notebook](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook)
    - [Safety](https://2013.igem.org/Team%3AHokkaidoU_Japan/Safety)
* [Human Practice](https://2013.igem.org/Team%3AHokkaidoU_Japan/Human_Practice)
  + [Education](https://2013.igem.org/Team%3AHokkaidoU_Japan/Education)
    - [University Festival](https://2013.igem.org/Team%3AHokkaidoU_Japan/Education#University_Festival)
    - [Highschool](https://2013.igem.org/Team%3AHokkaidoU_Japan/Education#Highschool)
  + [Survey](https://2013.igem.org/Team%3AHokkaidoU_Japan/Survey)
    - [University Festival](https://2013.igem.org/Team%3AHokkaidoU_Japan/Survey#University_Festival)
    - [Highschool](https://2013.igem.org/Team%3AHokkaidoU_Japan/Survey#Highschool)
* [About Us](https://2013.igem.org/Team%3AHokkaidoU_Japan/About_Us)
  + [Acknowledgements](https://2013.igem.org/Team%3AHokkaidoU_Japan/About_Us#Acknowledgements)
  + [Team](https://2013.igem.org/Team%3AHokkaidoU_Japan/About_Us#Team)

# Maestro E. coli

## Notebook

![](https://static.igem.org/mediawiki/2013/e/ea/HokkaidoU2013_Maestro_Header.png)

# Protocols

* [Protocols](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/Protocols)

# Lab Documents

## Promoter team

* [oripro(K1084007)-B0034-lacZ-B0015](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/Promoter_oripro%28K1084007%29-B0034-lacZ-B0015)
* [RandomPromtoer-B0034-mRFP1-B0015](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/Promoter_RandomPromtoer-B0034-mRFP1-B0015)
* [RandomPromtoer-B0034-LacZ-B0015](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/Promoter_RandomPromtoer-B0034-LacZ-B0015)
* [Weak Promoter-I732020](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/Promoter_WeakPromoter-I732020)
* [R0010, R0040-I732020](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/Promoter_R0010%2CR0040-I732020)
* [K1084001~010-B0034-lacZ-B0015](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/Promoter_K1084001~010-B0034-lacZ-B0015)
* [Construct Promoter Optimization Kit](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/Promoter_Construct_Promoter_Optimization_Kit)

## RBS team

* [SD8](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/RBS_SD8)
* [SD8-1C3 Replacement](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/RBS_SD8-1C3-Replacement)
* [SD2, 4, 6](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/RBS_SD2%2C4%2C6)
* [SD2, 4, 6 Reconstruction](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/RBS_SD2%2C4%2C6-Reconstruction)
* [SD2, 4, 6-1C3 Replacement](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/RBS_SD2%2C4%2C6-1C3-Replacement)
* [SD2, 4, 6, 8-3K3 Replacement](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/RBS_SD2%2C4%2C6%2C8-3K3-Replacement)

## Color team

* [R0040-B0034-K592011-B0015](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/Color_R0040-B0034-K592011-B0015)
* [R0040-B0034-(K592010, K592012, K864401)-B0015](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/Color_R0040-B0034-%28K592010%2C%20K592012%2C%20K864401%29-B0015)
* [R0040-S05192-B0015](https://2013.igem.org/Team%3AHokkaidoU_Japan/Notebook/Color_R0040-S05192-B0015)

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