iGEM 2018 — Team NCTU_Formosa

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# Team:NCTU Formosa/Notebook

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[Team](https://2017.igem.org/Team%3ANCTU_Formosa/Team)
[Collaborations](https://2017.igem.org/Team%3ANCTU_Formosa/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3ANCTU_Formosa/Description)
[Design](https://2017.igem.org/Team%3ANCTU_Formosa/Design)
[Experiments](https://2017.igem.org/Team%3ANCTU_Formosa/Experiments)
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[InterLab](https://2017.igem.org/Team%3ANCTU_Formosa/InterLab)
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[Model](https://2017.igem.org/Team%3ANCTU_Formosa/Model)
[Results](https://2017.igem.org/Team%3ANCTU_Formosa/Results)
[Demonstrate](https://2017.igem.org/Team%3ANCTU_Formosa/Demonstrate)
[Improve](https://2017.igem.org/Team%3ANCTU_Formosa/Improve)
[Attributions](https://2017.igem.org/Team%3ANCTU_Formosa/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3ANCTU_Formosa/Parts)
[Basic Parts](https://2017.igem.org/Team%3ANCTU_Formosa/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3ANCTU_Formosa/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3ANCTU_Formosa/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3ANCTU_Formosa/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3ANCTU_Formosa/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3ANCTU_Formosa/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3ANCTU_Formosa/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3ANCTU_Formosa/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3ANCTU_Formosa/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3ANCTU_Formosa/Hardware)
[Measurement](https://2017.igem.org/Team%3ANCTU_Formosa/Measurement)
[Model](https://2017.igem.org/Team%3ANCTU_Formosa/Model)
[Plant](https://2017.igem.org/Team%3ANCTU_Formosa/Plant)
[Software](https://2017.igem.org/Team%3ANCTU_Formosa/Software)

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* [Home](https://2018.igem.org/Team%3ANCTU_Formosa)
* Project

  [Description](https://2018.igem.org/Team%3ANCTU_Formosa/Project/Description)

  [Design](https://2018.igem.org/Team%3ANCTU_Formosa/Applied_Design)

  [IoT System](https://2018.igem.org/Team%3ANCTU_Formosa/Hardware)

  [Demonstration](https://2018.igem.org/Team%3ANCTU_Formosa/Demonstrate)

  [Entrepreneurship](https://2018.igem.org/Team%3ANCTU_Formosa/Entrepreneurship)

  [InterLab](https://2018.igem.org/Team%3ANCTU_Formosa/InterLab)
* Dry Lab

  [Overview](https://2018.igem.org/Team%3ANCTU_Formosa/Model)

  [Microbiota Prediction](https://2018.igem.org/Team%3ANCTU_Formosa/Dry_Lab/Microbiota_Prediciton)

  [Peptide Prediction](https://2018.igem.org/Team%3ANCTU_Formosa/Dry_Lab/Peptide_Prediction)

  [Growth Model](https://2018.igem.org/Team%3ANCTU_Formosa/Dry_Lab/Growth_Model)

  [Productivity Model](https://2018.igem.org/Team%3ANCTU_Formosa/Dry_Lab/Productivity_Model)

  [NGS Data Analysis](https://2018.igem.org/Team%3ANCTU_Formosa/Dry_Lab/NGS_Data_Analysis)
* Wet Lab

  [Experiment Design](https://2018.igem.org/Team%3ANCTU_Formosa/Wet_Lab)

  [Protein Expression](https://2018.igem.org/Team%3ANCTU_Formosa/Wet_Lab/Expression)

  [Functional Analysis](https://2018.igem.org/Team%3ANCTU_Formosa/Wet_Lab/Functional_Analysis)

  [Curcumin Bio-sensor](https://2018.igem.org/Team%3ANCTU_Formosa/Wet_Lab/Curcumin_Biosensor)

  [Safety](https://2018.igem.org/Team%3ANCTU_Formosa/Wet_Lab/Safety)
* Parts

  [Parts](https://2018.igem.org/Team%3ANCTU_Formosa/Parts)

  [Basic Parts](https://2018.igem.org/Team%3ANCTU_Formosa/Basic_Part)

  [Composite Parts](https://2018.igem.org/Team%3ANCTU_Formosa/Composite_Part)

  [Part Collection](https://2018.igem.org/Team%3ANCTU_Formosa/Part_Collection)

  [Improvement](https://2018.igem.org/Team%3ANCTU_Formosa/Improve)
* Human Practice

  [Overview](https://2018.igem.org/Team%3ANCTU_Formosa/Human_Practices)

  [Education &
  Public Engagement](https://2018.igem.org/Team%3ANCTU_Formosa/Public_Engagement)

  [Integrated HP](https://2018.igem.org/Team%3ANCTU_Formosa/Human_Practices/Integrated)

  [Collaboration](https://2018.igem.org/Team%3ANCTU_Formosa/Collaborations)
* Team

  [Team Introduction](https://2018.igem.org/Team%3ANCTU_Formosa/Team_Member)

  [Attributions](https://2018.igem.org/Team%3ANCTU_Formosa/Attributions)
* Notebook

  [Lab Note](https://2018.igem.org/Team%3ANCTU_Formosa/Notebook)

  [Protocol](https://2018.igem.org/Team%3ANCTU_Formosa/Protocol)
* [Achievements](https://2018.igem.org/Team%3ANCTU_Formosa/Achievements)

☰

[HOME](https://2018.igem.org/Team%3ANCTU_Formosa)

Project

[Description](https://2018.igem.org/Team%3ANCTU_Formosa/Project/Description)
[Design](https://2018.igem.org/Team%3ANCTU_Formosa/Applied_Design)
[IoT System](https://2018.igem.org/Team%3ANCTU_Formosa/Hardware)
[Demostration](https://2018.igem.org/Team%3ANCTU_Formosa/Demonstrate)
[Entrepreneurship](https://2018.igem.org/Team%3ANCTU_Formosa/Entrepreneurship)
[InterLab](https://2018.igem.org/Team%3ANCTU_Formosa/InterLab)

Dry Lab

[Overview](https://2018.igem.org/Team%3ANCTU_Formosa/Model)
[Microbiota Prediction](https://2018.igem.org/Team%3ANCTU_Formosa/Dry_Lab/Microbiota_Prediciton)
[Peptide Prediction](https://2018.igem.org/Team%3ANCTU_Formosa/Dry_Lab/Peptide_Prediction)
[Growth Model](https://2018.igem.org/Team%3ANCTU_Formosa/Dry_Lab/Growth_Model)
[Productivity Model](https://2018.igem.org/Team%3ANCTU_Formosa/Dry_Lab/Productivity_Model)
[NGS Data Analysis](https://2018.igem.org/Team%3ANCTU_Formosa/Dry_Lab/NGS_Data_Analysis)

Wet Lab

[Experiment Design](https://2018.igem.org/Team%3ANCTU_Formosa/Wet_Lab)
[Protein Expression](https://2018.igem.org/Team%3ANCTU_Formosa/Wet_Lab/Expression)
[Functional Analysis](https://2018.igem.org/Team%3ANCTU_Formosa/Wet_Lab/Functional_Analysis)
[Curcumin Bio-sensor](https://2018.igem.org/Team%3ANCTU_Formosa/Wet_Lab/Curcumin_Biosensor)
[Safety](https://2018.igem.org/Team%3ANCTU_Formosa/Wet_Lab/Safety)

Parts

[Parts](https://2018.igem.org/Team%3ANCTU_Formosa/Parts)
[Basic Parts](https://2018.igem.org/Team%3ANCTU_Formosa/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ANCTU_Formosa/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ANCTU_Formosa/Part_Collection)
[Improvement](https://2018.igem.org/Team%3ANCTU_Formosa/Improve)

Human Practice

[Overview](https://2018.igem.org/Team%3ANCTU_Formosa/Human_Practices)
[Education and Public Engagement](https://2018.igem.org/Team%3ANCTU_Formosa/Public_Engagement)
[Integrated HP](https://2018.igem.org/Team%3ANCTU_Formosa/Human_Practices/Integrated)
[Collaboration](https://2018.igem.org/Team%3ANCTU_Formosa/Collaborations)

Team

[Team Introduction](https://2018.igem.org/Team%3ANCTU_Formosa/Team_Member)
[Attributions](https://2018.igem.org/Team%3ANCTU_Formosa/Attributions)

Notebook

[Lab Note](https://2018.igem.org/Team%3ANCTU_Formosa/Notebook)
[Protocol](https://2018.igem.org/Team%3ANCTU_Formosa/Protocol)

[Achievements](https://2018.igem.org/Team%3ANCTU_Formosa/Achievements)

![](https://static.igem.org/mediawiki/2018/3/31/T--NCTU_Formosa--Notebook_cover.png)
[![](https://static.igem.org/mediawiki/2018/4/4c/T--NCTU_Formosa--Notebook_Lab_Note.png)](https://2018.igem.org/Team%3ANCTU_Formosa/Notebook)
[![](https://static.igem.org/mediawiki/2018/4/4f/T--NCTU_Formosa--Notebook_Lab_protocol.png)](https://2018.igem.org/Team%3ANCTU_Formosa/Protocol)

# Wet Lab

* [Jul](#item-1)
* [Aug](#item-2)
* [Sep](#item-3)
* [Oct](#item-4)

| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
| --- | --- | --- | --- | --- | --- | --- |
| [1](#July-1) | [2](#July-2) | [3](#July-3) | [4](#July-4) | [5](#July-5) | [6](#July-6) | [7](#July-7) |
| [8](#July-8) | [9](#July-9) | [10](#July-10) | [11](#July-11) | [12](#July-12) | [13](#July-13) | [14](#July-14) |
| [15](#July-15) | [16](#July-16) | [17](#July-17) | [18](#July-18) | [19](#July-19) | [20](#July-20) | [21](#July-21) |
| [22](#July-22) | [23](#July-23) | [24](#July-24) | [25](#July-25) | [26](#July-26) | [27](#July-27) | [28](#July-28) |
| [29](#July-29) | [30](#July-30) | [31](#July-31) |  |  |  |  |

| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
| --- | --- | --- | --- | --- | --- | --- |
|  |  |  | [1](#August-1) | [2](#August-2) | [3](#August-3) | [4](#August-4) |
| [5](#August-5) | [6](#August-6) | [7](#August-7) | [8](#August-8) | [9](#August-9) | [10](#August-10) | [11](#August-11) |
| [12](#August-12) | [13](#August-13) | [14](#August-14) | [15](#August-15) | [16](#August-16) | [17](#August-17) | [18](#August-18) |
| [19](#August-19) | [20](#August-20) | [21](#August-21) | [22](#August-22) | [23](#August-23) | [24](#August-24) | [25](#August-25) |
| [26](#August-26) | [27](#August-27) | [28](#August-28) | [29](#August-29) | [30](#August-30) | [31](#August-31) |  |

| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
| --- | --- | --- | --- | --- | --- | --- |
|  |  |  |  |  |  | [1](#September-1) |
| [2](#September-2) | [3](#September-3) | [4](#September-4) | [5](#September-5) | [6](#September-6) | [7](#September-7) | [8](#September-8) |
| [9](#September-9) | [10](#September-10) | [11](#September-11) | [12](#September-12) | [13](#September-13) | [14](#September-14) | [15](#September-15) |
| [16](#September-16) | [17](#September-17) | [18](#September-18) | [19](#September-19) | [20](#September-20) | [21](#September-21) | [22](#September-22) |
| [23](#September-23) | [24](#September-24) | [25](#September-25) | [26](#September-26) | [27](#September-27) | [28](#September-28) | [29](#September-29) |
| [30](#September-30) |  |  |  |  |  |  |

| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
| --- | --- | --- | --- | --- | --- | --- |
|  | [1](#October-1) | [2](#October-2) | [3](#October-3) | [4](#October-4) | [5](#October-5) | [6](#October-6) |
| [7](#October-7) | [8](#October-8) | [9](#October-9) | [10](#October-10) | [11](#October-11) | [12](#October-12) | [13](#October-13) |
| [14](#October-14) | [15](#October-15) | [16](#October-16) | [17](#October-17) | [18](#October-18) | [19](#October-19) | 20 |
| 21 | 22 | 23 | 24 | 25 | 26 | 27 |
| 28 | 29 | 30 | 31 |  |  |  |

## July 1

---

Cloning

* Received and resuspended of the IDT DNA fragments
* Sample

+ GS linker+αS1-casein
+ Enterocin B
+ Enterocin 96
+ Bovicin HJ50
+ Durancin TW49M
+ Lacticin Z
+ Leucocyclicin Q

* Received and resuspended of the IDT DNA fragments

## July 2

---

None

## July 3

---

Growth curve exp.

* Observe the growth population of *Bacillus subtilis* in different temperature Condition: 37°C, 32°C, 27°C

## July 4

---

Cloning gene of Curcumin bio-sensor

* Doing the PCR and clean-up to amplify the DNA fragment. Digesting pET30a backbone and ligating it with αS1-casein+GS linker and T7 promoter.
* Sample

+ pET30a backbone
+ DNA segment of αS1-casein+GS linker and T7 promoter.

## July 5

---

Cloning gene of Curcumin bio-sensor Expression

* Transforming the ligation product of the Curcumin bio-sensor into *E. coli* BL21 DE3 Cultivating the culture, preparing *E. coli* culture glycerol stocks, and doing the mini preparation.
* Sample

+ fresh colony of *E. coli* BL21 DE3 carrying the backbone pET30a containing αS1-casein + GS linker and T7 promotor gene from an overnight plate

## July 6

---

None

## July 7

---

Expression of αS1-casein

* Cultivating *E. coli* culture containing target protein αS1-casein from the *E. coli* glycerol stock and inducing it with IPTG to produce αS1-casein.
* Purifying the protein by His-Tag.

## July 8

---

Growth curve exp

* Observe the growth population of *Bacillus subtilis* in different pH Condition: pH = 4, 5, 6, 7, 8

## July 9

---

None

## July 10

---

Chip Production

* Preparing 18 chips for bio-sensor sensitivity pretest
  1. Dip the gold chips in 10mM Mua, RT for 4hrs.
  2. Wash the chips with 95% EtOH three times and dry.
  3. Add EDC+NHS mixture (100+100mM in DDW) on chips, RT for 1hrs.
  4. DDW rinse the chips and dry.
  5. Add αS1-casein on chips, RT for 1hrs.
  6. Wash with PBS three times and dry.
  6. Dip the chips in blocking solution, RT for 1.5hrs.
  7. Wash with PBS three times and dry.

## July 11

---

Bio-sensor sensitivity pretest

* Using DPV(Differential Pulse Voltammetry) to check whether our bio-sensor can detect curcumin.
  1. Add the diluted curcumin samples on our bio-sensor to react for 30min.
  2. Rinse with wash buffer and dry the chips.
  3. Wash the reference and counter electrodes with DDW, and dry them.
  4. Set up the three electrodes system within electrocheFunctional analysisal cell.
  5. Use the prototype of electrocheFunctional analysisal machine to measure the DPV method

## July 12

---

Bio-sensor sensitivity assay –Determine Standard Curve and Create the Formula

* Detecting the diluted standard curcumin samples by curcumin bio-sensor and made the standard curve and doing the polynomial curve fitting.

## July 13

---

Bio-sensor sensitivity assay -Detecting the real Samples from Turmeric Root

* Milling the turmeric root and dividing the powder into two groups. One of them was added with extraction buffer but not underwent the extraction protocol, and the other was added with extraction buffer but underwent the extraction process

## July 14

---

None

## July 15

---

Expression

* Preparing competent cell *E. coli* ER2566

Growth curve exp

* Observe the growth population of *Bacillus subtilis* in different salinity condition: 0.17M, 0.25M, 0.5M, 0.75M, 1.0M

## July 16

---

Cloning

* Amplify the insert gene (Pfu PCR)
* Electrophoresis (To check PCR products)
* Sample

+ Lacticin Z + pTXB1
+ Bovincin HJ50+ pTXB1

## July 17

---

Cloning

* Digestion of PCR products (Including insert and backbone pTXB1)
* Ligation of digestion products (Ligase the DNA fragment into backbone pTXB1)
* Sample

+ Lacticin Z + pTXB1
+ Bovincin HJ50+ pTXB1

## July 18

---

Cloning

* Transformation of ligation products (Transform into *E. coli* DH5α)
* Taq PCR (PCR of ligation products to amplify insert gene)
* Electrophoresis (To check PCR products)
* Sample

+ Lacticin Z + pTXB1
+ Bovincin HJ50+ pTXB1

## July 19

---

Cloning

* Cultivation
* Miniprep (Purify Plasmid)
* Sample

+ Lacticin Z + pTXB1
+ Bovincin HJ50+ pTXB1

## July 20

---

Cloning

* Sequencing plasmid
* Sample

+ Lacticin Z + pTXB1
+ Bovincin HJ50+ pTXB1

## July 21

---

None

## July 22

---

Cloning

* Amplify the insert gene (Pfu PCR)
* Electrophoresis (To check PCR products)
* Digestion of PCR products (Including insert and backbone pTXB1)
* Ligation of digestion products (Ligase the DNA fragment into backbone pTXB1)
* Sample

+ Leucocyclicin Q + pTXB1

## July 23

---

Cloning

* Transformation of ligation products (Transform into *E. coli* DH5α)
* Taq PCR (PCR of ligation products to amplify insert gene)
* Electrophoresis (To check PCR products)
* Sample

+ Leucocyclicin Q + pTXB1

## July 24

---

Cloning

* Cultivation
* Miniprep (Purify Plasmid)
* Sample

+ Leucocyclicin Q + pTXB1

## July 25

---

Cloning

* Sequencing plasmid
* Sample

+ Leucocyclicin Q + pTXB1

## July 26

---

None

## July 27

---

None

## July 28

---

None

## July 29

---

Expression

* Transformation (Transform correct plasmid into *E. coli* ER2566)

+ Leucocyclicin Q + pTXB1

## July 30

---

None

## July 31

---

None

## August 1

---

None

## August 2

---

None

## August 3

---

None

## August 4

---

None

## August 5

---

Expression

* Transformation (Transform correct plasmid into *E. coli* ER2566)
* sample

+ Leucocyclicin Q + pTXB1

## August 6

---

Expression

* Cultivation (Cultivation of *E. coli* ER2566 colonies for IPTG induction)
* IPTG Induction (To test the O.D. levels of *E. coli* ER2566 to induce)Condition: O.D. = 0.5, 1.3
* sample

+ Leucocyclicin Q + pTXB1

## August 7

---

Expression

* SDS-PAGE (To check the protein production after induction)
* sample

+ Leucocyclicin Q + pTXB1

## August 8

---

None

## August 9

---

None

## August 10

---

None

## August 11

---

Cloning

* Amplify the insert gene (Pfu PCR)
* Electrophoresis (To check PCR products)
* sample

+ Enterocin B
+ Enterocin 96
+ Durancin TW49M

## August 12

---

Cloning

* Digestion of PCR products (Including insert and backbone pTXB1)
* Ligation of digestion products (Ligase the DNA fragment into backbone pTXB1)
* Transformation of ligation products (Transform into *E. coli* DH5α)
* sample

+ Enterocin B + pTXB1
+ Enterocin 96 + pTXB1
+ Durancin TW-49M + pTXB1

Growth curve

* Observe the growth of *Bacillus subtilis* in different temperature, pH, salinityCondition:
* 1.Temp: 37°C , pH:7 , salinity: 0.17M
* 2.Temp: 30°C , pH:9 , salinity: 0.5M
* 3.Temp: 25°C , pH:5 , salinity: 0.25M

## August 13

---

Cloning

* Taq PCR (PCR of ligation products to amplify insert gene)
* Electrophoresis (To check PCR products)
* sample

+ Enterocin B + pTXB1
+ Enterocin 96 + pTXB1
+ Durancin TW-49M + pTXB1

## August 14

---

Cloning

* Cultivation
* Miniprep (Purify Plasmid)
* Sample

+ Enterocin B + pTXB1
+ Enterocin 96 + pTXB1
+ Durancin TW-49M + pTXB1

Expression

* Transformation (Transform correct plasmid into *E. coli* ER2566)to test different cultivation temperature

## August 15

---

Cloning

* Sequencing plasmid
* Sample

+ Enterocin B + pTXB1
+ Enterocin 96 + pTXB1
+ Durancin TW-49M + pTXB1

Expression

* Cultivation (Cultivation of *E. coli* ER2566 colonies for IPTG induction)
* IPTG Induction (To test the temperature of cultivation after induction)Condition: temp = 37, 30, 13.5°C

## August 16

---

Expression

* SDS-PAGE (To check the protein production after induction)Check different temperature
* IPTG Induction (To test the concentration of IPTG)Condition: concentration = 200, 400, 600, 800, 1000μM

## August 17

---

Expression

* SDS-PAGE (To check the protein production after induction) -> Check different IPTG concentration

## August 18

---

Transformation

* Transforming backbone pTXB1 into *E. coli* ER2566 to produce protein as our negative control in verifying the function of our target peptide.
* Transforming backbone pTXB1 containing bacteriocin gene into *E. coli* ER2566 to produce our target protein as a bio-stimulator.
* Sample

+ backbone pTXB1 mini
+ backbone pTXB1 containing Enterocin B gene mini
+ backbone pTXB1 containing Enterocin 96 gene mini
+ backbone pTXB1 containing Leucocyclicin Q gene mini

Cultivation

+ Cultivate the *E. coli* ER2566 at 37°C cultivating *Bacillus subtilis* ER2566 at 37°C carrying the backbone pTXB1 to produce protein as our negative control in verifying the function of our target peptide.
+ Cultivate the *E. coli* ER2566 at 37°C cultivating *E. coli* ER2566 at 37°C carrying the backbone pTXB1 containing bacteriocin gene to produce our target protein as a bio-stimulator.
+ Sample

- Fresh colony of *E. coli* ER2566 carrying the backbone pTXB1 from an overnight plate
- Fresh colony of *E. coli* ER2566 carrying the backbone pTXB1 containing Enterocin B gene from an overnight plate
- Fresh colony of *E. coli* ER2566 carrying the backbone pTXB1 containing Enterocin 96 gene from an overnight plate
- Fresh colony of *E. coli* ER2566 carrying the backbone pTXB1 containing Leucocyclicin Q gene from an overnight plate

IPTG Induction

- Inducting *E. coli* ER2566 carrying the backbone pTXB1 after cultivating at 37°C to produce protein as our negative control in verifying the function of our target peptide
- Inducting *E. coli* ER2566 carrying the backbone pTXB1 containing bacteriocin gene after cultivating at 37°C to produce our target protein as a bio-stimulator.
- Sample

* Culture of *E. coli* ER2566 carrying the backbone pTXB1
* Culture of *E. coli* ER2566 carrying the backbone pTXB1 containing Enterocin B gene
* Culture of *E. coli* ER2566 carrying the backbone pTXB1 containing Enterocin 96 gene
* Culture of *E. coli* ER2566 carrying the backbone pTXB1 containing Leucocyclicin Q gene

## August 19

---

Cloning

* Digestion of PCR products (Including insert and backbone pET30a)
* Ligation of digestion products (Ligase the DNA fragment into backbone pET30a)
* Sample(8/17)

+ Enterocin B + pET30a
+ Enterocin 96 + pET30a
+ Bovicin HJ50 + pET30a
+ Durancin TW-49M + pET30a
+ Lacticin Z + pET30a
+ Leucocyclicin Q + pET30a

Sonication

* Breaking *E. coli* ER2566 carrying the backbone pTXB1 and pTXB1 containing bacteriocin gene after cultivating to harvest the protein by sonication.
* Sample

+ Culture of *E. coli* ER2566 carrying the backbone pTXB1 induced by IPTG
+ Culture of *E. coli* ER2566 carrying the backbone pTXB1 containing Enterocin B gene induced by IPTG
+ Culture of *E. coli* ER2566 carrying the backbone pTXB1 containing Enterocin 96 gene induced by IPTG
+ Culture of *E. coli* ER2566 carrying the backbone pTXB1 containing Leucocyclicin Q gene induced by IPTG

Protein quantification & SDS-PAGE

* Quantificating the protein expressing the backbone pTXB1 and pTXB1 containing bacteriocin gene by Bradford method and running SDS-PAGE to check the protein expression.
* Sample

+ Protein expressing the backbone pTXB1 induced by IPTG
+ Protein expressing the backbone pTXB1 containing Enterocin B gene induced by IPTG
+ Protein expressing the backbone pTXB1 containing Enterocin 96 gene induced by IPTG
+ Protein expressing the backbone pTXB1 containing Leucocyclicin Q gene induced by IPTG

## August 20

---

Cloning

* Transformation of ligation products (Transform into *E. coli* DH5α)
* Taq PCR (PCR of ligation products to amplify insert gene)
* Electrophoresis (To check PCR products)
* Sample

+ Enterocin B + pET30a
+ Enterocin 96 + pET30a
+ Bovicin HJ50 + pET30a
+ Durancin TW-49M + pET30a
+ Lacticin Z + pET30a
+ Leucocyclicin Q + pET30a

## August 21

---

Cloning

* Cultivation
* Miniprep (Purify Plasmid)
* Sample

+ Enterocin B + pET30a
+ Enterocin 96 + pET30a
+ Bovicin HJ50 + pET30a
+ Durancin TW-49M + pET30a
+ Lacticin Z + pET30a
+ Leucocyclicin Q + pET30a

## August 22

---

Cloning

* Sequencing plasmid
* Sample

+ Enterocin B + pET30a
+ Enterocin 96 + pET30a
+ Bovicin HJ50 + pET30a
+ Durancin TW-49M + pET30a
+ Lacticin Z + pET30a
+ Leucocyclicin Q + pET30a

## August 23

---

None

## August 24

---

Transformation

* Transforming backbone pTXB1 containing bacteriocin gene into *E. coli* ER2566 to produce our target protein as a bio-stimulator.
* Sample

+ backbone pTXB1 containing Bovicin HJ50 gene mini
+ backbone pTXB1 containing Durancin TW-49M gene mini

Cultivation

* Cultivate the *E. coli* ER2566 at 37°C cultivating *E. coli* ER2566 at 37°C carrying the backbone pTXB1 containing bacteriocin gene to produce our target protein as a bio-stimulator.
* Sample

+ Fresh colony of *E. coli* ER2566 carrying the backbone pTXB1 containing Bovicin HJ50 gene from an overnight plate
+ Fresh colony of *E. coli* ER2566 carrying the backbone pTXB1 containing Durancin TW-49M gene from an overnight plate

IPTG Induction

* Inducting *E. coli* ER2566 carrying the backbone pTXB1 containing bacteriocin gene after cultivating at 37°C to produce our target protein as a bio-stimulator.
* Sample

+ Culture of *E. coli* ER2566 carrying the backbone pTXB1 containing Bovicin HJ50 gene
+ Culture of *E. coli* ER2566 carrying the backbone pTXB1 containing Durancin TW-49M gene

## August 25

---

Sonication

* Breaking *E. coli* ER2566 carrying the backbone pTXB1 containing bacteriocin gene after cultivating to harvest the protein by sonication.
* Sample

+ Culture of *E. coli* ER2566 carrying the backbone pTXB1 containing Bovicin HJ50 gene induced by IPTG
+ Culture of *E. coli* ER2566 carrying the backbone pTXB1 containing Durancin TW-49M gene induced by IPTG

Protein quantification & SDS-PAGE

* Quantificating the protein expressing the backbone pTXB1 containing bacteriocin gene by Bradford method and running SDS-PAGE to check the protein expression.
* Sample

+ Protein expressing the backbone pTXB1 containing Bovicin HJ50 gene induced by IPTG
+ Protein expressing the backbone pTXB1 containing Durancin TW-49M gene induced by IPTG

## August 26

---

None

## August 27

---

None

## August 28

---

None

## August 29

---

None

## August 30

---

None

## August 31

---

None

## September 1

---

None

## September 2

---

None

## September 3

---

None

## September 4

---

None

## September 5

---

None

## September 6

---

Transformation

* Transforming backbone pTXB1 containing Lacticin Z gene into *E. coli* ER2566 to produce our target protein as a bio-stimulator.
* Sample

+ backbone pTXB1 containing Lacticin Z gene mini

Cultivation

* Cultivate the *E. coli* ER2566 at 37°C cultivating *Bacillus subtilis* ER2566 at 37°C carrying the backbone pTXB1 containing Lacticin Z gene to produce our target protein as a bio-stimulator.
* Sample

+ Fresh colony of *E. coli* ER2566 carrying the backbone pTXB1 containing Lacticin Z gene from an overnight plate

IPTG Induction

* Inducting *E. coli* ER2566 carrying the backbone pTXB1 containing Lacticin Z gene after cultivating at 37°C to produce our target protein as a bio-stimulator.
* Sample

+ Culture of *E. coli* ER2566 carrying the backbone pTXB1 containing Lacticin Z gene

## September 7

---

Sonication

* Breaking *E. coli* ER2566 carrying the backbone pTXB1 containing Lacticin Z gene after cultivating to harvest the protein by sonication.
* Sample

+ Culture of *E. coli* ER2566 carrying the backbone pTXB1 containing Lacticin Z gene induced by IPTG

Protein quantification & SDS-PAGE

* Quantificating the protein expressing the backbone pTXB1 containing Lacticin Z gene by Bradford method and running SDS-PAGE to check the protein expression.
* Sample

+ Protein expressing the backbone pTXB1 containing Lacticin Z gene induced by IPTG

## September 8

---

None

## September 9

---

None

## September 10

---

None

## September 11

---

None

## September 12

---

None

## September 13

---

None

## September 14

---

None

## September 15

---

None

## September 16

---

None

## September 17

---

None

## September 18

---

None

## September 19

---

None

## September 20

---

None

## September 21

---

Functional analysis test

* Test the function of Lacticin Z (produced by *E. coli* ER2566) by elisa reader(triplicate)
* Sample

+ Control:
  1. *Bacillus subtilis* + Ampicillin
  2. *Bacillus subtilis* + Production of the pTXB1 backbone induced by IPTG
  3. *Bacillus subtilis* + LB broth
  4. LB broth for background
+ Test:
  1. *Bacillus subtilis* + Production of the pTXB1 backbone containing Lacticin Z gene induced by IPTG

## September 22

---

None

## September 23

---

None

## September 24

---

None

## September 25

---

None

## September 26

---

None

## September 27

---

None

## September 28

---

None

## September 29

---

None

## September 30

---

None

## October 1

---

None

## October 2

---

None

## October 3

---

None

## October 4

---

None

## October 5

---

Transformation

* Transforming backbone pTXB1 into *E. coli* Rosetta-Gami to produce protein as our negative control in verifying the function of our target peptide.
* Transforming backbone pTXB1 containing bacteriocin gene into *E. coli* Rosetta-Gami to produce our target protein as a bio-stimulator.
* Sample

+ backbone pTXB1 mini
+ backbone pTXB1 containing Enterocin B gene mini
+ backbone pTXB1 containing Enterocin 96 gene mini
+ backbone pTXB1 containing Bovicin HJ50 gene mini
+ backbone pTXB1 containing Durancin TW-49M gene mini
+ backbone pTXB1 containing Lacticin Z gene mini
+ backbone pTXB1 containing Leucocyclicin Q gene mini

## October 6

---

Cultivation

* Cultivate the *E. coli* ER2566 at 37°C cultivating *E. coli* Rosetta-Gami at 37°C carrying the backbone pTXB1 to produce protein as our negative control in verifying the function of our target peptide.
* Cultivate the *E. coli* ER2566 at 37°C cultivating *E. coli* Rosetta-Gami at 37°C carrying the backbone pTXB1 containing bacteriocin gene produce our target protein as a bio-stimulator.
* Sample

+ Fresh colony of *E. coli* Rosetta-Gami carrying the backbone pTXB1 from an overnight plate
+ Fresh colony of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Enterocin B gene from an overnight plate
+ Fresh colony of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Enterocin 96 gene from an overnight plate
+ Fresh colony of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Leucocyclicin Q gene from an overnight plate

IPTG Induction

* Cultivate the *E. coli* ER2566 at 37°C cultivating *E. coli* Rosetta-Gami at 37°C carrying the backbone pTXB1 to produce protein as our negative control in verifying the function of our target peptide.
* Inducting *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing bacteriocin gene after cultivating at 37°C produce our target protein as a bio-stimulator.
* Sample

+ Fresh colony of *E. coli* Rosetta-Gami carrying the backbone pTXB1 from an overnight plate
+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Enterocin B gene
+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Enterocin 96 gene
+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Leucocyclicin Q gene

## October 7

---

Cultivation

* Cultivate the *E. coli* ER2566 at 37°C cultivating *E. coli* Rosetta-Gami at 37°C carrying the backbone pTXB1 containing bacteriocin gene produce our target protein as a bio-stimulator.
* Sample

+ Fresh colony of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Bovicin HJ50 gene from an overnight plate
+ Fresh colony of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Durancin TW-49M gene from an overnight plate
+ Fresh colony of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Lacticin Z gene from an overnight plate

IPTG Induction

* Inducting *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing bacteriocin gene after cultivating at 37°C produce our target protein as a bio-stimulator.
* Sample

+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Bovicin HJ50 gene
+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Durancin TW-49M gene
+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Lacticin Z gene

Sonication

* Breaking *E. coli* Rosetta-Gami carrying the backbone pTXB1 and pTXB1 containing bacteriocin gene after cultivating to harvest the protein by sonication.
* Sample

+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 induced by IPTG
+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Enterocin B gene induced by IPTG
+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Enterocin 96 gene induced by IPTG
+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Leucocyclicin Q gene induced by IPTG

Protein quantification & SDS-PAGE

* Quantificating the protein expressing the backbone pTXB1 and pTXB1 containing bacteriocin gene by Bradford method and running SDS-PAGE to check the protein expression.
* Sample

+ Protein expressing the backbone pTXB1 induced by IPTG
+ Protein expressing the backbone pTXB1 containing Enterocin B gene induced by IPTG
+ Protein expressing the backbone pTXB1 containing Enterocin 96 gene induced by IPTG
+ Protein expressing the backbone pTXB1 containing Leucocyclicin Q gene induced by IPTG

## October 8

---

Sonication

* Breaking *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing bacteriocin gene after cultivating to harvest the protein by sonication.
* Sample

+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Bovicin HJ50 gene induced by IPTG
+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Durancin TW-49M gene induced by IPTG
+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Lacticin Z gene induced by IPTG
+ Culture of *E. coli* Rosetta-Gami carrying the backbone pTXB1 containing Leucocyclicin Q gene induced by IPTG

Protein quantification & SDS-PAGE

* Quantificating the protein expressing the backbone pTXB1 containing bacteriocin gene by Bradford method and running SDS-PAGE to check the protein expression.
* Sample

+ Protein expressing the backbone pTXB1 containing Bovicin HJ50 gene induced by IPTG
+ Protein expressing the backbone pTXB1 containing Durancin TW-49M gene induced by IPTG
+ Protein expressing the backbone pTXB1 containing Lacticin Z gene induced by IPTG
+ Protein expressing the backbone pTXB1 containing Leucocyclicin Q gene induced by IPTG

## October 9

---

Functional analysis test

* Test the function of bacteriocins (produced by *E. coli* Rosetta-gami) by elisa reader(triplicate)
* Sample

+ Control:
  1. *Bacillus subtilis* + Ampicillin
  2. *Bacillus subtilis* + Production of the pTXB1 backbone induced by IPTG
  3. *Bacillus subtilis* + LB broth
  4. LB broth for background
+ Test:
  1. *Bacillus subtilis* + Production of the pTXB1 backbone containing Enterocin B gene induced by IPTG
  2. *Bacillus subtilis* + Production of the pTXB1 backbone containing Enterocin 96 gene induced by IPTG
  3. *Bacillus subtilis* + Production of the pTXB1 backbone containing Lacticin Z gene induced by IPTG
  4. *Bacillus subtilis* + Production of the pTXB1 backbone containing Durancin TW-49M gene induced by IPTG

## October 10

---

Functional analysis test

* Test the function of bacteriocins (produced by *E. coli* Rosetta-gami) by elisa reader(triplicate)
* Sample

+ Control:
  1. *Bacillus subtilis* + Ampicillin
  2. *Bacillus subtilis* + Production of the pTXB1 backbone induced by IPTG
  3. *Bacillus subtilis* + LB broth
  4. LB broth for background
+ Test:
  1. *Bacillus subtilis* + Production of the pTXB1 backbone containing Bovicin HJ50 gene induced by IPTG
  2. *Bacillus subtilis* + Production of the pTXB1 backbone containing Leucocyclicin Q gene induced by IPTG

## October 11

---

None

## October 12

---

Functional analysis test

* Test the function of bacteriocins (produced by *E. coli* Rosetta-gami) by elisa reader(Dose response assessment)
* Sample

+ Control:
  1. *Bacillus subtilis* + Ampicillin
  2. *Bacillus subtilis* + Production of the pTXB1 backbone induced by IPTG
  3. *Bacillus subtilis* + LB broth
  4. LB broth for background
+ Test:
  1. *Bacillus subtilis* + Production of the pTXB1 backbone containing Enterocin B gene induced by IPTG
  2. *Bacillus subtilis* + Production of the pTXB1 backbone containing Enterocin 96 gene induced by IPTG
  3. *Bacillus subtilis* + Production of the pTXB1 backbone containing Lacticin Z gene induced by IPTG
  4. *Bacillus subtilis* + Production of the pTXB1 backbone containing Durancin TW-49M gene induced by IPTG

## October 13

---

None

## October 14

---

None

## October 15

---

Functional analysis test

* Test the function of bacteriocins (produced by *E. coli* Rosetta-gami) by elisa reader(Dose response assessment)
* Sample

+ Control:
  1. *Bacillus subtilis* + Ampicillin
  2. *Bacillus subtilis* + Production of the pTXB1 backbone induced by IPTG
  3. *Bacillus subtilis* + LB broth
  4. LB broth for background
+ Test:
  1. *Bacillus subtilis* + Production of the pTXB1 backbone containing Bovicin HJ50 gene induced by IPTG
  2. *Bacillus subtilis* + Production of the pTXB1 backbone containing Leucocyclicin Q gene induced by IPTG

Functional analysis test(inhibition zone)

* Test the function of Enterocin B (produced by *E. coli* Rosetta-gami) by LB Agar + Ampicillin plate
* Sample

+ The *Bacillus subtilis* on the plate
+ Control:
  1. Ampicillin
  2. Production of the pTXB1 backbone induced by IPTG
  + Test:
    1. Production of the pTXB1 backbone containing Enterocin B gene induced by IPTG

## October 16

---

Functional analysis test(inhibition zone)

* Test the function of bacteriocins (produced by *E. coli* Rosetta-gami) by LB Agar + Ampicillin plate
* Sample

+ The *Bacillus subtilis* on the plate
+ Control:
  1. Ampicillin
  2. Production of the pTXB1 backbone induced by IPTG
  + Test:
    1. Production of the pTXB1 backbone containing Bovicin HJ50 gene induced by IPTG
    2. Production of the pTXB1 backbone containing Lacticin Z gene induced by IPTG

## October 17

---

Functional analysis test

* Test the function of bacteriocins (produced by *E. coli* Rosetta-gami) by elisa reader(Dose response assessment)
* Sample

+ Control:
  1. *Bacillus subtilis* + Ampicillin
  2. *Bacillus subtilis* + Production of the pTXB1 backbone induced by IPTG
  3. *Bacillus subtilis* + LB broth
  + Test:
    1. *Bacillus subtilis* + Production of the pTXB1 backbone containing Durancin TW-49M gene induced by IPTG

## October 18

---

None

## October 19

---

None

![](https://static.igem.org/mediawiki/2018/9/96/T--NCTU_Formosa--Footer2.0.png)


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:NCTU Formosa/Experiments

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![](https://static.igem.org/mediawiki/igem.org/3/3b/Menu_icon.svg)

[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3ANCTU_Formosa)
[HOME](https://2018.igem.org/Team%3ANCTU_Formosa)

TEAM

[Team Members](https://2018.igem.org/Team%3ANCTU_Formosa/Team)
[Collaborations](https://2018.igem.org/Team%3ANCTU_Formosa/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ANCTU_Formosa/Description)
[Design](https://2018.igem.org/Team%3ANCTU_Formosa/Design)
[Experiments](https://2018.igem.org/Team%3ANCTU_Formosa/Experiments)
[Notebook](https://2018.igem.org/Team%3ANCTU_Formosa/Notebook)
[InterLab](https://2018.igem.org/Team%3ANCTU_Formosa/InterLab)
[Model](https://2018.igem.org/Team%3ANCTU_Formosa/Model)
[Results](https://2018.igem.org/Team%3ANCTU_Formosa/Results)
[Demonstrate](https://2018.igem.org/Team%3ANCTU_Formosa/Demonstrate)
[Improve](https://2018.igem.org/Team%3ANCTU_Formosa/Improve)
[Attributions](https://2018.igem.org/Team%3ANCTU_Formosa/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ANCTU_Formosa/Parts)
[Basic Parts](https://2018.igem.org/Team%3ANCTU_Formosa/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ANCTU_Formosa/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ANCTU_Formosa/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ANCTU_Formosa/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ANCTU_Formosa/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ANCTU_Formosa/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ANCTU_Formosa/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ANCTU_Formosa/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ANCTU_Formosa/Hardware)
[Measurement](https://2018.igem.org/Team%3ANCTU_Formosa/Measurement)
[Model](https://2018.igem.org/Team%3ANCTU_Formosa/Model)
[Plant](https://2018.igem.org/Team%3ANCTU_Formosa/Plant)
[Software](https://2018.igem.org/Team%3ANCTU_Formosa/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=NCTU_Formosa)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:NCTU Formosa/Results

![](https://static.igem.org/mediawiki/2018/4/4f/2018_igem_mobile_menu_logo.svg)

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[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3ANCTU_Formosa)
[HOME](https://2018.igem.org/Team%3ANCTU_Formosa)

TEAM

[Team Members](https://2018.igem.org/Team%3ANCTU_Formosa/Team)
[Collaborations](https://2018.igem.org/Team%3ANCTU_Formosa/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ANCTU_Formosa/Description)
[Design](https://2018.igem.org/Team%3ANCTU_Formosa/Design)
[Experiments](https://2018.igem.org/Team%3ANCTU_Formosa/Experiments)
[Notebook](https://2018.igem.org/Team%3ANCTU_Formosa/Notebook)
[InterLab](https://2018.igem.org/Team%3ANCTU_Formosa/InterLab)
[Model](https://2018.igem.org/Team%3ANCTU_Formosa/Model)
[Results](https://2018.igem.org/Team%3ANCTU_Formosa/Results)
[Demonstrate](https://2018.igem.org/Team%3ANCTU_Formosa/Demonstrate)
[Improve](https://2018.igem.org/Team%3ANCTU_Formosa/Improve)
[Attributions](https://2018.igem.org/Team%3ANCTU_Formosa/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ANCTU_Formosa/Parts)
[Basic Parts](https://2018.igem.org/Team%3ANCTU_Formosa/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ANCTU_Formosa/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ANCTU_Formosa/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ANCTU_Formosa/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ANCTU_Formosa/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ANCTU_Formosa/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ANCTU_Formosa/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ANCTU_Formosa/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ANCTU_Formosa/Hardware)
[Measurement](https://2018.igem.org/Team%3ANCTU_Formosa/Measurement)
[Model](https://2018.igem.org/Team%3ANCTU_Formosa/Model)
[Plant](https://2018.igem.org/Team%3ANCTU_Formosa/Plant)
[Software](https://2018.igem.org/Team%3ANCTU_Formosa/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=NCTU_Formosa)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)