iGEM 2017 — Team Lethbridge

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# Team:Lethbridge/Notebook

[![](https://static.igem.org/mediawiki/2017/archive/3/35/20171024022846%21Next_vivo_caption_white.png)](https://2017.igem.org/Team%3ALethbridge)

* Project
  + [Description](https://2017.igem.org/Team%3ALethbridge/Description)
  + [Design](https://2017.igem.org/Team%3ALethbridge/Design)
  + [Demonstrate](https://2017.igem.org/Team%3ALethbridge/Demonstrate)
  + [Results](https://2017.igem.org/Team%3ALethbridge/Results)
  + [Collaborations](https://2017.igem.org/Team%3ALethbridge/Collaborations)
* [Parts](https://2017.igem.org/Team%3ALethbridge/Parts)
  + [Overview](https://2017.igem.org/Team%3ALethbridge/Parts)
  + [Part Collection](https://2017.igem.org/Team%3ALethbridge/Part_Collection)
  + [Composite](https://2017.igem.org/Team%3ALethbridge/Composite_Part)
  + [Improved Part](https://2017.igem.org/Team%3ALethbridge/Improve)
  + [Basic](https://2017.igem.org/Team%3ALethbridge/Basic_Part)
* Human Practices
  + [Overview](https://2017.igem.org/Team%3ALethbridge/Human_Practices)
  + [User Groups - Silver](https://2017.igem.org/Team%3ALethbridge/HP/Silver)
  + [Integrated HP - Gold](https://2017.igem.org/Team%3ALethbridge/HP/Gold_Integrated)
  + [Biosecurity Software](https://2017.igem.org/Team%3ALethbridge/Software)
  + [Engagement](https://2017.igem.org/Team%3ALethbridge/Engagement)
* Lab
  + [Experiments](https://2017.igem.org/Team%3ALethbridge/Experiments)
  + [Notebook](https://2017.igem.org/Team%3ALethbridge/Notebook)
  + [Safety](https://2017.igem.org/Team%3ALethbridge/Safety)
  + [InterLab](https://2017.igem.org/Team%3ALethbridge/InterLab)
* [Team](https://2017.igem.org/Team%3ALethbridge/Team)
  + [Team Members](https://2017.igem.org/Team%3ALethbridge/Team)
  + [Attributions](https://2017.igem.org/Team%3ALethbridge/Attributions)
  + [Sponsors](https://2017.igem.org/Team%3ALethbridge/Attributions#Sponsors)

![](https://static.igem.org/mediawiki/2017/e/e6/Banner_LAnotebook.png)

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# Team:Lethbridge/Experiments

[![](https://static.igem.org/mediawiki/2017/archive/3/35/20171024022846%21Next_vivo_caption_white.png)](https://2017.igem.org/Team%3ALethbridge)

* Project
  + [Description](https://2017.igem.org/Team%3ALethbridge/Description)
  + [Design](https://2017.igem.org/Team%3ALethbridge/Design)
  + [Demonstrate](https://2017.igem.org/Team%3ALethbridge/Demonstrate)
  + [Results](https://2017.igem.org/Team%3ALethbridge/Results)
  + [Collaborations](https://2017.igem.org/Team%3ALethbridge/Collaborations)
* [Parts](https://2017.igem.org/Team%3ALethbridge/Parts)
  + [Overview](https://2017.igem.org/Team%3ALethbridge/Parts)
  + [Part Collection](https://2017.igem.org/Team%3ALethbridge/Part_Collection)
  + [Composite](https://2017.igem.org/Team%3ALethbridge/Composite_Part)
  + [Improved Part](https://2017.igem.org/Team%3ALethbridge/Improve)
  + [Basic](https://2017.igem.org/Team%3ALethbridge/Basic_Part)
* Human Practices
  + [Overview](https://2017.igem.org/Team%3ALethbridge/Human_Practices)
  + [User Groups - Silver](https://2017.igem.org/Team%3ALethbridge/HP/Silver)
  + [Integrated HP - Gold](https://2017.igem.org/Team%3ALethbridge/HP/Gold_Integrated)
  + [Biosecurity Software](https://2017.igem.org/Team%3ALethbridge/Software)
  + [Engagement](https://2017.igem.org/Team%3ALethbridge/Engagement)
* Lab
  + [Experiments](https://2017.igem.org/Team%3ALethbridge/Experiments)
  + [Notebook](https://2017.igem.org/Team%3ALethbridge/Notebook)
  + [Safety](https://2017.igem.org/Team%3ALethbridge/Safety)
  + [InterLab](https://2017.igem.org/Team%3ALethbridge/InterLab)
* [Team](https://2017.igem.org/Team%3ALethbridge/Team)
  + [Team Members](https://2017.igem.org/Team%3ALethbridge/Team)
  + [Attributions](https://2017.igem.org/Team%3ALethbridge/Attributions)
  + [Sponsors](https://2017.igem.org/Team%3ALethbridge/Attributions#Sponsors)

[top](#top)

![](https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png)

## Protocols

|  |  |
| --- | --- |
| [Agarose Gel Extraction](https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf) | [Colony PCR](https://static.igem.org/mediawiki/2017/6/66/Colony_PCR.pdf) |
| [DNA Purification from Enzymatic Reactions](https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf) | [*In vitro* Transcription](https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf) |
| [LB Media](https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf) | [Ligations](https://static.igem.org/mediawiki/2017/9/9d/Ligation.pdf) |
| [PCR Amplification](https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf) | [PCR Product Purification](https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf) |
| [Phenol/Chloroform Extraction and Ethanol Precipitation](https://static.igem.org/mediawiki/2017/f/f2/PhenolFChloroform_extraction_%26_ethanol_percipitation.pdf) | [pJET Cloning](https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf) |
| [Plasmid Miniprep](https://static.igem.org/mediawiki/2017/0/08/Plasmid_Miniprep.pdf) | [Preparation of Chemical Competent Cells](https://static.igem.org/mediawiki/2017/7/78/Preparation_of_Chemical_Competent_Cells.pdf) |
| [Protein Overexpression](https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf) | [PURExpress NEB Kit](https://static.igem.org/mediawiki/2017/5/5f/PURExpress.pdf) |
| [Purification of HexaHistidine Tagged Proteins](https://static.igem.org/mediawiki/2017/f/fc/Purification_of_Poly-Histidine_Tagged_Proteins.pdf) | [Restriction Enzyme Digest and Dephosphorylation](https://static.igem.org/mediawiki/2017/1/10/Restriction_Enzyme_Digest_and_Dephosphorylation.pdf) |
| [T7- RNA Polymerase Purification](https://static.igem.org/mediawiki/2017/2/2a/T7-RNA_Polymerase_Purification.pdf) | [Transformation](https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf) |
| [MS2-tRNA Affinity Chromatography Purification](https://static.igem.org/mediawiki/2017/c/cd/MS2-tRNA_Complex_Purification.pdf) |  |

## Products/Reagents

| Enzymes | Buffers/Reagents | Other Products |
| --- | --- | --- |
| Shrimp Alkaline Phosphatase | Affinity Chromatography Buffers | Ni²+ Affinity Resin |
| DNA Blunting Enzyme | IPTG | Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’ |
| Phusion Polymerase Master Mix | Kanomycin / Chloramphenicol / Ampicillin | Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’ |
| PFU Polymerase | Dimethyl Sulfur Dioxide | Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’ |
| T7 RNA Polymerase | Competent Cell Buffers | Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’ |
| T7 DNA Polymerase | T7 RNA Polymerase Purification Buffers | Dialysis Tubing |
| PURExpressKit (New England Biolabs) | Phenol |  |
| XbaI (NEB) | Chloroform |  |
| SpeI (NEB) | 10x PFU Buffer |  |
| PstI (NEB) | NTP |  |
| EcoRI (NEB) | GMP |  |
| Dream Taq DNA Polymerase | 10x Taq Buffer |  |
| Taq DNA Polymerase | Ligase Buffer |  |
| RNase A | SDS-PAGE Reagents |  |
| TraB | Urea PAGE reagents |  |
| iPPase | Agarose |  |
| RNase Inhibitor | Agar |  |
| DNaseI | Tryptone |  |
|  | Yeast Extract |  |
|  | TAKMz |  |
|  | BioBasic Plasmid DNA miniprep Kit |  |
|  | Imidazole |  |

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[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Lethbridge/Results

[![](https://static.igem.org/mediawiki/2017/archive/3/35/20171024022846%21Next_vivo_caption_white.png)](https://2017.igem.org/Team%3ALethbridge)

* Project
  + [Description](https://2017.igem.org/Team%3ALethbridge/Description)
  + [Design](https://2017.igem.org/Team%3ALethbridge/Design)
  + [Demonstrate](https://2017.igem.org/Team%3ALethbridge/Demonstrate)
  + [Results](https://2017.igem.org/Team%3ALethbridge/Results)
  + [Collaborations](https://2017.igem.org/Team%3ALethbridge/Collaborations)
* [Parts](https://2017.igem.org/Team%3ALethbridge/Parts)
  + [Overview](https://2017.igem.org/Team%3ALethbridge/Parts)
  + [Part Collection](https://2017.igem.org/Team%3ALethbridge/Part_Collection)
  + [Composite](https://2017.igem.org/Team%3ALethbridge/Composite_Part)
  + [Improved Part](https://2017.igem.org/Team%3ALethbridge/Improve)
  + [Basic](https://2017.igem.org/Team%3ALethbridge/Basic_Part)
* Human Practices
  + [Overview](https://2017.igem.org/Team%3ALethbridge/Human_Practices)
  + [User Groups - Silver](https://2017.igem.org/Team%3ALethbridge/HP/Silver)
  + [Integrated HP - Gold](https://2017.igem.org/Team%3ALethbridge/HP/Gold_Integrated)
  + [Biosecurity Software](https://2017.igem.org/Team%3ALethbridge/Software)
  + [Engagement](https://2017.igem.org/Team%3ALethbridge/Engagement)
* Lab
  + [Experiments](https://2017.igem.org/Team%3ALethbridge/Experiments)
  + [Notebook](https://2017.igem.org/Team%3ALethbridge/Notebook)
  + [Safety](https://2017.igem.org/Team%3ALethbridge/Safety)
  + [InterLab](https://2017.igem.org/Team%3ALethbridge/InterLab)
* [Team](https://2017.igem.org/Team%3ALethbridge/Team)
  + [Team Members](https://2017.igem.org/Team%3ALethbridge/Team)
  + [Attributions](https://2017.igem.org/Team%3ALethbridge/Attributions)
  + [Sponsors](https://2017.igem.org/Team%3ALethbridge/Attributions#Sponsors)

[top](#top)

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## Overview

![](https://static.igem.org/mediawiki/2017/c/cb/T--Lethbridge--gold.png)

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