iGEM 2014 — Team AHUT_China

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# Team:AHUT China/Notebook

### From 2014.igem.org

* [HOME](https://2014.igem.org/Team%3AAHUT_China)
* [TEAM](https://2014.igem.org/Team%3AAHUT_China/Team)
* [PROJECT](https://2014.igem.org/Team%3AAHUT_China/Project)
* [HUMAN PRACTICES](https://2014.igem.org/Team%3AAHUT_China/HumanPractices)
* [SAFETY](https://2014.igem.org/Team%3AAHUT_China/Safety)
* [NOTEBOOK](https://2014.igem.org/Team%3AAHUT_China/Notebook)

A man can fail many times, but he isn't a failure until he begins to blame somebody else.

---J. Burroughs. Averican naturalist

NOTEBOOK

![](https://static.igem.org/mediawiki/2014/2/22/AHUT_CHINA_2014_triangle.png)

![](https://static.igem.org/mediawiki/2014/8/85/AHUT_CHINA_2014_notebook_pic_1.png)

PROTOCOLS

![](https://static.igem.org/mediawiki/2014/2/22/AHUT_CHINA_2014_triangle.png)

Taq Plus PCR

MasterMix
0.1 U Taq Plus Polymerase/μl
500 μM dNTP each
20 mM Tris-HCl (pH8.3)
100 mM KCl
3 mM MgCl2
![](https://static.igem.org/mediawiki/2014/a/ae/AHUT_CHINA_2014_notebook_pic_2.jpg)

![](https://static.igem.org/mediawiki/2014/b/b0/AHUT_CHINA_2014_notebook_pic_3.jpg)

TIANgel Midi Purification Kit

![](https://static.igem.org/mediawiki/2014/3/31/AHUT_CHINA_2014_notebook_pic_4.jpg)

DNA MW Standard Marker

![](https://static.igem.org/mediawiki/2014/1/16/AHUT_CHINA_2014_notebook_pic_5.png)

LB media

|  |  |
| --- | --- |
| 1L LB medium | 1% |
| Yeast Extract | 0.5% |
| aCl | 1% |

E.coli

DH5a
Top10

Plasmids

![](https://static.igem.org/mediawiki/2014/f/f4/AHUT_CHINA_2014_notebook_pic_6.jpg)

pUC57:http://www.ncbi.nlm.nih.gov/nuccore/Y14837.1

PSB1C3

DNA Ligation Kit

![](https://static.igem.org/mediawiki/2014/2/26/AHUT_CHINA_2014_notebook_pic_7.jpg)

Enzyme Cutting

![](https://static.igem.org/mediawiki/2014/5/50/AHUT_CHINA_2014_notebook_pic_8.jpg)

Lab Pictures

![](https://static.igem.org/mediawiki/2014/2/22/AHUT_CHINA_2014_triangle.png)

![](https://static.igem.org/mediawiki/2014/6/6a/AHUT_CHINA_2014_notebook_pic_9.jpg)
![](https://static.igem.org/mediawiki/2014/2/2b/AHUT_CHINA_2014_notebook_pic_10.jpg)

![](https://static.igem.org/mediawiki/2014/d/d1/AHUT_CHINA_2014_notebook_pic_11.jpg)
![](https://static.igem.org/mediawiki/2014/8/86/AHUT_CHINA_2014_notebook_pic_15.jpg)

![](https://static.igem.org/mediawiki/2014/4/47/AHUT_CHINA_2014_notebook_pic_12.jpg)
![](https://static.igem.org/mediawiki/2014/6/6e/AHUT_CHINA_2014_notebook_pic_13.jpg)

![](https://static.igem.org/mediawiki/2014/0/07/AHUT_CHINA_2014_notebook_pic_14.jpg)

* + TEAM
  + [Members](https://2014.igem.org/Team%3AAHUT_China/Team#m)
  + [Instructors](https://2014.igem.org/Team%3AAHUT_China/Team#i)
  + [Attributions](https://2014.igem.org/Team%3AAHUT_China/Team#a)
  + [Acknowledgement](https://2014.igem.org/Team%3AAHUT_China/Team#ac)
* + PROJECT
  + [Background](https://2014.igem.org/Team%3AAHUT_China/Project#b)
  + [Design](https://2014.igem.org/Team%3AAHUT_China/Project#d)
  + [Results&future work](https://2014.igem.org/Team%3AAHUT_China/Project#r)
  + [Parts](https://2014.igem.org/Team%3AAHUT_China/Project#p)
* + HUMAN PRACTICES
  + [Military Training](https://2014.igem.org/Team%3AAHUT_China/HumanPractices#m)
  + [Society](https://2014.igem.org/Team%3AAHUT_China/HumanPractices#s)
  + [APP and Sina Weibo](https://2014.igem.org/Team%3AAHUT_China/HumanPractices#a)
  + [Help Each Other](https://2014.igem.org/Team%3AAHUT_China/HumanPractices#h)
  + [School qualify](https://2014.igem.org/Team%3AAHUT_China/HumanPractices#sc)
* + SAFETY
  + [Safety](https://2014.igem.org/Team%3AAHUT_China/Safety#s)
* + NOTEBOOK
  + [Notebook](https://2014.igem.org/Team%3AAHUT_China/Notebook#n)
  + [Protocols](https://2014.igem.org/Team%3AAHUT_China/Notebook#p)
  + [Lab Pictures](https://2014.igem.org/Team%3AAHUT_China/Notebook#l)

![](https://static.igem.org/mediawiki/2014/8/87/AHUT_CHINA_2014_index_foot_dash_gray.jpg)

Anhui University of Technology is located in the national civilized city, Nanjing metropolitan core cities - Ma'anshan City, Anhui Province. Anhui University of Technology is an engineering, metallurgical features, engineering, economics, management, arts, science, law and other disciplines coordinated development of key construction in Anhui multidisciplinary university, with "The Master, Bo" complete the multi-level education training system.

![](https://static.igem.org/mediawiki/2014/4/4a/AHUT_CHINA_2014_index_foot_line_gray.jpg)

Copyright 2014 © AHUT iGEM Team , All rights reserved .

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