iGEM 2017 — Team HokkaidoU_Japan

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# Team:HokkaidoU Japan/Notebook

![](https://static.igem.org/mediawiki/2017/thumb/3/39/TOPgazouT--HokkaidoU_Japan.png/800px-TOPgazouT--HokkaidoU_Japan.png)

* [HOME](https://2017.igem.org/Team%3AHokkaidoU_Japan)
* TEAM
  + [Team](https://2017.igem.org/Team%3AHokkaidoU_Japan/Team)
  + [Collaboration](https://2017.igem.org/Team%3AHokkaidoU_Japan/Collaborations)
  + [Attribution](https://2017.igem.org/Team%3AHokkaidoU_Japan/Attributions)
* Project
  + [Description](https://2017.igem.org/Team%3AHokkaidoU_Japan/Description)
  + [Design](https://2017.igem.org/Team%3AHokkaidoU_Japan/Design)
  + [Experiments](https://2017.igem.org/Team%3AHokkaidoU_Japan/Experiments)
  + [Results](https://2017.igem.org/Team%3AHokkaidoU_Japan/Results)
  + [Future Work](https://2017.igem.org/Team%3AHokkaidoU_Japan/Future_Work)
  + [Notebook](https://2017.igem.org/Team%3AHokkaidoU_Japan/Notebook)
* Parts
  + [Parts](https://2017.igem.org/Team%3AHokkaidoU_Japan/Parts)
* [Safety](https://2017.igem.org/Team%3AHokkaidoU_Japan/Safety)
* Human Practice
  + [Silver HP](https://2017.igem.org/Team%3AHokkaidoU_Japan/HP/Silver)

# Notebook

**8/21**

Transformation

Researcher: Kono, Esashi, Sugishita,
Shimada

Part: BBa\_R0010 on pSB1C3

**8/22**

Liquid Culture

Researcher: Kishimoto, Kono, Esashi,
Sugishita, Shimada

Part: BBa\_R0010 on pSB1C3

**8/23**

Mini-Prep

Researcher: Kishimoto, Kono, Esashi, Sugishita,
Shimada

Part: BBa\_R0010 on pSB1C3

Electrophoresis

Researcher: Kishimoto, Kono, Esashi, Sugishita,
Shimada

Part: BBa\_R0010 on pSB1C3

**8/24**

Liquid Culture

Researcher: Kono, Sugishita, Shimada

Part: BBa\_R0010 on pSB1C3

**8/25**

Mini-Prep

Researcher: Hirao, Kono, Kuroiwa, Nakamura,
Shimada

Part: BBa\_R0010 on pSB1C3

**8/30**

NanoDrop

Researcher: Kishimoto, Kono, Sugishita,
Sato

Part: BBa\_R0010 on pSB1C3

Digestion

Researcher: Kishimoto, Kono, Sugishita,
Sato

Part: BBa\_R0010 on pSB1C3

Electrophoresis

Researcher: Kishimoto, Kono, Sugishita,
Sato, Shimada, Takagi

Part: BBa\_R0010, pSB1C3

**8/31**

Digestion

Researcher: Hirao, Kishimoto, Kono,
Sugishita, Sato

Part: BBa\_R0010 on pSB1C3

Electrophoresis

Researcher: Hirao, Kishimoto, Kono,
Sugishita, Sato

Part: BBa\_R0010, pSB1C3

Liquid Culture

Researcher: Hirao, Kono, Shimada

Part: BBa\_R0010 on pSB1C3

**9/1**

Mini-Prep

Researcher: Hirao, Hirao, Sugishita, Sato, Shimada

Part: BBa\_R0010 on pSB1C3

Electrophoresis

Researcher: Hirao, Kono, Sugishita, Sato, Shimada

Part: BBa\_R0010 on pSB1C31

NanoDrop

Researcher:

Part: BBa\_R0010 on pSB1C31

Digestion

Researcher: Hirao, Kono, Sugishita, Sato, Shimada

Part: BBa\_R0010 on pSB1C3

Electrophoresis

Researcher: Hirao, Kono, Sugishita, Sato, Shimada

Part: BBa\_R0010, pSB1C3

Electrophoresis

Researcher:

Part: BBa\_R0010, pSB1C3

Gel Extraction

Researcher:

Part: BBa\_R0010, pSB1C3

Electrophoresis

Researcher:

Part: BBa\_R0010, pSB1C3

**9/6**

Transformation

Researcher: Hirao, Esashi, Noda, Sugishita,
Shimada

Part: BBa\_J23100 on J61002, BBa\_J23102 on
J61002, BBa\_J23104 on J61002, BBa\_K912000 on pSB1C3, BBa\_K912001 on pSB1C3

**9/7**

Liquid Culture

Researcher: Esashi

Part: BBa\_K912001 on pSB1C3

Transformation

Researcher: Hirao, Esashi

Part: BBa\_J23100 on J61002, BBa\_J23102 on
J61002, BBa\_J23104 on J61002

**9/8**

Mini-Prep

Researcher: Hirao, Kono, Esashi, Sugishita,
Kawata

Part: BBa\_K912001 on pSB1C3

Electrophoresis

Researcher: Hirao, Kono, Esashi, Sugishita

Part: BBa\_K912001 on pSB1C3

NanoDrop

Researcher: Hirao

Part: BBa\_K912001 on pSB1C3

Digestion

Researcher: Hirao

Part: BBa\_K912001 on pSB1C3, pUC19

Electrophoresis

Researcher: Hirao

Part: BBa\_K912001, pSB1C3, pUC19

**9/11**

Electrophoresis

Researcher: Hirao, Esashi

Part: BBa\_K912001, pSB1C3, pUC19

Transformation

Researcher: Hirao, Esashi, Sugishita

Part: pUC19

Electrophoresis

Researcher: Hirao, Esashi, Sugishita

Part: BBa\_K912001, pSB1C3, pUC19

Ethanol Precipitation

Researcher: Hirao, Esashi, Sugishita, Sato

Part: BBa\_K912001

Gel Extraction

Researcher: Hirao, Esashi, Sugishita, Sato

Part: BBa\_K912001

Electrophoresis

Researcher: Hirao, Esashi, Sugishita, Sato

Part: BBa\_K912001

Electrophoresis

Researcher: Hirao, Esashi, Sugishita, Sato

Part: BBa\_K912001

Liquid Culture

Researcher:

Part: BBa\_J23100 on J61002, BBa\_J23102 on J61002,
BBa\_J23104 on J61002

**9/12**

NanoDrop

Researcher: Hirao, Sato

Part: BBa\_K912001, pSB1C3

Mini-Prep

Researcher: Hirao, Esashi, Sugishita

Part: BBa\_J23100 on J61002, BBa\_J23102 on
J61002, BBa\_J23104 on J61002

Electrophoresis

Researcher: Hirao, Esashi, Sugishita

Part: BBa\_J23100 on J61002, BBa\_J23102 on
J61002, BBa\_J23104 on J61002

**9/13**

Digestion

Researcher: Hirao, Saito

Part: BBa\_K912001 on pSB1C3

Electrophoresis

Researcher: Hirao, Saito

Part: BBa\_K912001

Ethanol Precipitation

Researcher: Hirao, Noda, Saito

Part: BBa\_K912001

Electrophoresis

Researcher: Hirao,
Noda, Sito

Part: BBa\_K912001

Gel Extraction

Researcher: Hirao, Noda, Saito

Part: BBa\_K912001

**9/14**

NanoDrop

Researcher: Hirao, Noda, Saito

Part: BBa\_K912001

Electrophoresis

Researcher: Hirao, Kishimoto, Saito

Part: BBa\_K912001

Electrophoresis

Researcher: Hirao, Kishimoto, Saito

Part: BBa\_K912001

Mini-Prep

Researcher: Noda

Part: pUC19

Electrophoresis

Researcher: Hirao, Noda, Saito

Part: pUC19

NanoDrop

Researcher: Hirao

Part: pUC19

**9/15**

Digestion

Researcher: Hirao, Saito

Part: BBa\_K912001 on pSB1C3

Electrophoresis

Researcher: Hirao, Saito, Sugishita,
Shimada

Part: BBa\_K912001, pSB1C3

Ethanol Precipitation

Researcher: Sugishita

Part: BBa\_K912001 on pSB1C3

Gel Extraction

Researcher: Hirao, Shimada

Part: BBa\_K912001

Electrophoresis

Researcher:

Part: BBa\_K912001

**9/19**

Transformation

Researcher:

Part: BBa\_K912000 on pSB1C3

Liquid Culture

Researcher:

Part: BBa\_K912001 on pSB1C3

**9/20**

Mini-Prep

Researcher: Kono

Part: BBa\_K912001 on pSB1C3

Electrophoresis

Researcher: Kono

Part: BBa\_K912001 on pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa\_K912001 on pSB1C3

PCR

Researcher:

Part: BBa\_K912001 on pSB1C3

Electrophoresis

Researcher:

Part: BBa\_K912001 on pSB1C3

**9/20**

Liquid Culture

Researcher: Hirao

Part: BBa\_K912000 on pSB1C3

NanoDrop

Researcher: Kono

Part: BBa\_K912001 on pSB1C3

**9/21**

Mini-Prep

Researcher: Kono

Part: BBa\_K912000 on pSB1C3

Electrophoresis

Researcher: Kono, Sugishita

Part: BBa\_K912000 on pSB1C3

NanoDrop

Researcher: Sugishita

Part: BBa\_K912000 on pSB1C3

PCR

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

Electrophoresis

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

PCR Clean-up

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

NanoDrop

Researcher: Sugishita

Part: INSERT 5

Digestion

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

Digestion

Researcher: Kono, Esashi, Sugishita

Part: BBa\_K912001 on pSB1C3

**9/22**

Electrophoresis

Researcher: Kono, Esashi

Part: INSERT 5

**9/23**

PCR

Researcher: Kono, Esashi

Part: INSERT 5

PCR Clean-up

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

NanoDrop

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

Digestion

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

NanoDrop

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

Electrophoresis

Researcher: Kono, Esashi, Sugishita

Part: INSERT 5

Ethanol Precipitation

Researcher: Kono, Esashi, Sugishita, Sato

Part: INSERT 5

Gel Extraction

Researcher: Hirao, Kono, Esashi, Sugishita,
Sato

Part: INSERT 5

NanoDrop

Researcher: Hirao, Kono, Esashi, Sugishita,
Sato

Part: INSERT 5

Ligation

Researcher: Hirao, Kono, Esashi, Sugishita,
Sato

Part: pSB1C3

Electrophoresis

Researcher: Hirao, Esashi

Part: pSB1C3

Ligation

Researcher: Kono

Part: pSB1C3

Electrophoresis

Researcher: Kono

Part: pSB1C3

Ligation

Researcher: Kono

Part: BBa\_K912001, INSERT 5

**9/24**

PCR

Researcher: Hirao

Part: INSERT 5

PCR Clean-up

Researcher: Hirao

Part: INSERT 5

NanoDrop

Researcher: Hirao

Part: INSERT 5

Digestion

Researcher: Hirao

Part: INSERT 5

Digestion

Researcher: Hirao

Part: BBa\_K912001

Ethanol Precipitation

Researcher: Hirao

Part: BBa\_K912001

Gel Extraction

Researcher: Hirao, Nishimura

Part: BBa\_K912001, INSERT 5

Transformation

Researcher: Hirao, Nishimura

Part: BBa\_K912001 \_ INSERT 5

Gel Extraction

Researcher: Hirao, Nishimura

Part: BBa\_K912001, INSERT 5

NanoDrop

Researcher: Hirao

Part: BBa\_K912001, INSERT 5

Ligation

Researcher: Hirao

Part: BBa\_K912001, INSERT 5

Ethanol Precipitation

Researcher: Kono

Part: BBa\_K912001 \_ INSERT 5

Transformation

Researcher: Kono

Part: BBa\_K912001 \_ INSERT 5

**9/25**

Ligation

Researcher: Kamo,
Sugishita

Part: BBa\_K912001, INSERT 5

PCR

Researcher: Esashi, Saito

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Ligation

Researcher: Kamo,
Sugishita

Part: BBa\_K912001, INSERT 5

PCR Clean-up

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Electrophoresis

Researcher: Esashi,
Kamo, Saito, Sugishita

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Digestion

Researcher: Kono, Sugishita

Part: pSB1C3, INSERT 1

**9/26**

Transformation

Researcher: Kono

Part: pSB1C3 - INSERT 5

Ethanol Precipitation

Researcher: Hirao

Part: pSB1C3, INSERT 1

NanoDrop

Researcher: Kono,
Kamo, Saito

Part: pSB1C3, INSERT 1

Digestion

Researcher: Kono,
Kamo, Saito

Part: pSB1C3, INSERT 1

Digestion

Researcher: Kono, Saito

Part: BBa\_K912001, INSERT 5

Ethanol Precipitation

Researcher: Kono

Part: BBa\_K912001, INSERT 1

Ethanol Precipitation

Researcher: Saito

Part: BBa\_K912001, INSERT 5

Electrophoresis

Researcher: Saito

Part: BBa\_K912001, INSERT 1

PCR

Researcher: Hirao

Part: INSERT 5

Ethanol Precipitation

Researcher: Hirao, Kishimoto

Part: BBa\_K921000, BBa\_K912001

PCR

Researcher: Hirao, Esashi

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Ethanol Precipitation

Researcher: Esashi

Part: BBa\_K921000, BBa\_K912001

Digestion

Researcher: Hirao, Esashi

Part: BBa\_K921000, BBa\_K912001

PCR Clean-up

Researcher: Hirao, Esashi

Part: INSERT 5

NanoDrop

Researcher: Esashi

Part: INSERT 5

Ethanol Precipitation

Researcher: Hirao

Part: BBa\_K921000, BBa\_K912001

PCR Clean-up

Researcher: Esashi

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

PCR Clean-up

Researcher: Hirao

Part: INSERT 5

NanoDrop

Researcher: Hirao

Part: INSERT 5

NanoDrop

Researcher: Esashi

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

**9/27**

NanoDrop

Researcher: Hirao

Part: BBa\_K912001

Digestion

Researcher: Hirao, Esashi

Part: INSER T 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

Liquid Culture

Researcher: Hirao

Part: BBa\_K912000, BBa\_K912001

Gel Extract

Researcher: Hirao

Part: BBa\_K912000, BBa\_K912001

NanoDrop

Researcher: Hirao

Part: BBa\_K912000, BBa\_K912001

Electrophoresis

Researcher: Hirao

Part: INSER T 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

Ethanol Precipitation

Researcher: Hirao

Part: INSER T 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

Gel Extract

Researcher: Hirao

Part: INSER T 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

PCR

Researcher: Kono, Saito

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

Mini-Prep

Researcher: Kono, Saito

Part: BBa\_K912000, BBa\_K912001

PCR Clean-up

Researcher: Saito

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

Electrophoresis

Researcher: Kono, Saito

Part: BBa\_K912000, BBa\_K912001

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

PCR

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

**9/28**

PCR Clean-up

Researcher: Hirao, Kono

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3,
INSERT 4, INSERT 5

NanoDrop

Researcher: Kono

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3,
INSERT 4, INSERT 5

Ethanol Precipitation

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001

NanoDrop

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001

PCR

Researcher: Kono

Part: pSB1C3

PCR Clean-up

Researcher: Kono

Part: pSB1C3

Gel Extract

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

NanoDrop

Researcher: Kono

Part: pSB1C3

Electrophoresis

Researcher: Kono

Part: pSB1C3

PCR

Researcher: Esashi

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

PCR

Researcher: Esashi, Saito

Part: pSB1C3

Digestion

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

**9/29**

Electrophoresis

Researcher: Hirao

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3,
INSERT 4, INSERT 5

PCR Clean-up

Researcher: Hirao

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3,
INSERT 4, INSERT 5

Gel Extraction

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

NanoDrop

Researcher: Hirao

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3,
INSERT 4, INSERT 5

NanoDrop

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

Digestion

Researcher: Kono, Saito

Part: BBa\_K912000, BBa\_K912001

Mini-Prep

Researcher: Noda

Part: BBa\_K912000, BBa\_K912001

Ethanol Precipitation

Researcher: Kono, Esashi, Saito

Part: BBa\_K912000, BBa\_K912001

NanoDrop

Researcher: Kono, Esashi, Saito

Part: BBa\_K912000, BBa\_K912001

Digestion

Researcher: Esashi, Saito

Part: BBa\_K912000, BBa\_K912001

Digestion

Researcher: Esashi, Saito

Part: BBa\_K912000, BBa\_K912001

Gel Extraction

Researcher: Esashi, Saito

Part: BBa\_K912000, BBa\_K912001

NanoDrop

Researcher: Kono, Esashi

Part: BBa\_K912000, BBa\_K912001

**9/30**

Ligation

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5, BBa\_K912001 \_
INSERT 5

Digestion

Researcher: Hirao, Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

Digestion

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

Ethanol Precipitation

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001, INSERT 1,
INSERT 2, INSERT 3, INSERT 4, INSERT 5

Gel Extract

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001, INSERT 1,
INSERT 2, INSERT 3, INSERT 4, INSERT 5

NanoDrop

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001, INSERT 1,
INSERT 2, INSERT 3, INSERT 4, INSERT 5

Ethanol Precipitation

Researcher: Hirao

Part: BBa\_K912000 - INSERT 5, BBa\_K912001 \_
INSERT 5

PCR Clean-up

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

NanoDrop

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

PCR

Researcher: Hirao

Part: INSERT 2, INSERT 3, INSERRT 4

PCR Clean-up

Researcher: Hirao,
Kamo

Part: INSERT 2, INSERT 3, INSERRT 4

Electrophoresis

Researcher: Hirao,
Kamo

Part: INSERT 2, INSERT 3, INSERRT 4

NanoDrop

Researcher: Hirao,
Kamo

Part: INSERT 2, INSERT 3, INSERRT 4

Mini-Prep

Researcher: Hirao

Part: BBa\_K912000, BBa\_K912001

NanoDrop

Researcher: Hirao

Part: BBa\_K912000, BBa\_K912001

Ethanol Precipitation

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001

Digestion

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

Mini-Prep

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001

NanoDrop

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001

Ethanol Precipitation

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

Colony PCR

Researcher: Kono

Part: BBa\_K912000

Gel Extraction

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 4

**10/1**

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

PCR Clean-up

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Digestion

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Mini-Prep

Researcher: Kono

Part: BBa\_K912000 on pSB1C3, BBa\_K912001 on
pSB1C3

NanoDrop

Researcher: Kono

Part: BBa\_K912000 on pSB1C3, BBa\_K912001 on
pSB1C3

**10/2**

Digestion

Researcher: Hirao, Kono

Part: BBa\_K912000 on pSB1C3, BBa\_K912001 on
pSB1C3

Ethanol Precipitation

Researcher: Hirao, Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Gel Extraction

Researcher: Hirao, Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

NanoDrop

Researcher: Hirao, Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Gel Extraction

Researcher: Hirao, Kono

Part: BBa\_K912000 on pSB1C3, BBa\_K912001 on
pSB1C3

NanoDrop

Researcher: Hirao, Kono

Part: BBa\_K912000, BBa\_K912001

Ligation

Researcher: Hirao, Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, BBa\_K912000

Ligation

Researcher: Hirao

Part: BBa\_K912001, INSERT 5

**10/3**

Colony PCR

Researcher: Kono

Part: BBa\_K912000

Transformation

Researcher: Kono, Sugishita

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 3 on pSB1C3, INSERT 1 \_ INSERT 4 on pSB1C3, INSERT 5 on K912001

PCR

Researcher: Hirao

Part: BBa\_K912001, INSERT 5

**10/4**

Electrophoresis

Researcher: Kishimoto, Kono

Part: BBa\_K912001, INSERT 5

Colony PCR

Researcher: Kishimoto, Kono

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 3 on pSB1C3, INSERT 1 \_ INSERT 4 on pSB1C3

Liquid Culture

Researcher: Hirao

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 3 on pSB1C3, INSERT 1 \_ INSERT 4 on pSB1C3, BBa\_K912001 \_ INSERT 5
on pSB1C3

Mini-Prep

Researcher: Kamo,
Sugishita

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 3 on pSB1C3, INSERT 1 \_ INSERT 4 on pSB1C3, BBa\_K912001 \_ INSERT 5
on pSB1C3

Electrophoresis

Researcher: Sugishita

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 3 on pSB1C3, INSERT 1 \_ INSERT 4 on pSB1C3, BBa\_K912001 \_ INSERT 5
on pSB1C3

**10/6**

Digestion

Researcher: Kono

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 3 on pSB1C3, INSERT 1 \_ INSERT 4 on pSB1C3, BBa\_K912001 \_ INSERT 5
on pSB1C3

Electrophoresis

Researcher: Kono

Part: INSERT 1 \_ INSERT 2, INSERT 1 \_
INSERT 3, INSERT 1 \_ INSERT 4, BBa\_K912001 \_ INSERT 5

Electrophoresis

Researcher: Kono

Part: INSERT 1 \_ INSERT 2, INSERT 1 \_
INSERT 3, INSERT 1 \_ INSERT 4, BBa\_K912001 \_ INSERT 5

Ligation

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

**10/7**

Electrophoresis

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, INSERT 5

Digestion

Researcher: Kono

Part: BBa\_K912000 on pSB1C3, BBa\_K912001 on
pSB1C3

PCR

Researcher: Noda

Part: INSERT 1, INSERT 2, INSERT 3

Ethanol Precipitation

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001

Electrophoresis

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3

Ethanol Precipitation

Researcher: Kono

Part: BBa\_K912000 on pSB1C3

NanoDrop

Researcher: Kono

Part: BBa\_K912000 on pSB1C3

PCR Clean-up

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3

Gel Extraction

Researcher: Kono

Part: BBa\_K912000

Electrophoresis

Researcher: Kono

Part: BBa\_K912000

**10/8**

Gel Extraction

Researcher: Hirao

Part: BBa\_K912000, BBa\_K912001

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3

Electrophoresis

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3,
BBa\_K912000, BBa\_K912001

NanoDrop

Researcher: Hirao

Part: BBa\_K912000, BBa\_K912001

Ligation

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001

PCR Clean-up

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3

**10/9**

Electrophoresis

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3

Gel Extraction

Researcher: Hirao, Kono

Part: INSERT 5

NanoDrop

Researcher: Hirao

Part: INSERT 5

Liquid Culture

Researcher: Hirao

Part: BBa\_K912000 on pSB1C3

Ligation

Researcher: Kono

Part: INSERT 5

Ligation

Researcher: Kono

Part: INSERT 5, BBa\_K912001

Ethanol Precipitation

Researcher:

Part: INSERT 5, BBa\_K912001

Electrophoresis

Researcher: Hirao,
Kamo

Part: INSERT 5

Mini-Prep

Researcher: Hirao

Part: BBa\_K912000 on pSB1C3

NanoDrop

Researcher: Hirao

Part: BBa\_K912000 on pSB1C3

Transformation

Researcher: Kono

Part: BBa\_K912001 \_ INSERT 5

**10/10**

Liquid Culture

Researcher: Hirao

Part: BBa\_R0010 on pSB1C3, BBa\_K592009 on
pSB1C3

PCR

Researcher: Kono

Part: INSERT 3, INSERT 4, INSERT 5

Electrophoresis

Researcher: Kono

Part: INSERT 3, INSERT 4, INSERT 5

Colony PCR

Researcher: Kono

Part: BBa\_K912001 - INSERT 5

Digestion

Researcher: Kono

Part: BBa\_K912000 on pSB1C3, pUC19

Electrophoresis

Researcher: Kono

Part: BBa\_K912000 on pSB1C3, pUC19

Mini-Prep

Researcher: Kono

Part: BBa\_R0010 on pSB1C3, BBa\_K592009 on
pSB1C3

NanoDrop

Researcher: Kono

Part: BBa\_R0010 on pSB1C3, BBa\_K592009 on
pSB1C3

PCR

Researcher: Kono

Part: INSERT 3, INSERT 4

Electrophoresis

Researcher: Kono

Part: INSERT 3, INSERT 4, BBa\_K912001 - INSERT

Digestion

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3,
BBa\_R0010, BBa\_K592009

PCR Clean-up

Researcher: Hirao

Part: INSERT 3, INSERT 4, INSERT 5

NanoDrop

Researcher: Hirao

Part: INSERT 3, INSERT 4, INSERT 5

Digestion

Researcher: Kono

Part: INSERT 4, INSERT 5

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3,
BBa\_R0010, BBa\_K592009

**10/11**

Gel Extraction

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3,
BBa\_R0010, BBa\_K592009

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 4, INSERT 5

Gel Extraction

Researcher: Hirao, Kono

Part: INSERT 4, INSERT 5

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3,
BBa\_R0010, BBa\_K592009

NanoDrop

Researcher: Kono

Part: INSERT 4, INSERT 5

Ligation

Researcher: Kono

Part: INSERT 1 \_ INSERT 2. INSERT 1 \_
INSERT 3, INSERT 1 \_ INSERT 4

Ligation

Researcher: Kono

Part: BBa\_R0010

**10/11**

PCR

Researcher: Kono

Part: INSERT 1, INSERT 3

Ethanol Precipitation

Researcher: Kono

Part: INSERT 1 \_ INSERT 2. INSERT 1 \_
INSERT 3, INSERT 1 \_ INSERT 4

Electrophoresis

Researcher: Kono

Part: BBa\_R0010

Transformation

Researcher: Kono

Part: INSERT 1 \_ INSERT 2. INSERT 1 \_
INSERT 3, INSERT 1 \_ INSERT 4

Electrophoresis

Researcher: Kono

Part: INSERT 1, INSERT 3

Mini-Prep

Researcher: Hirao

Part: BBa\_K912001 \_ INSERT 5 on pSB1C3,
BBa\_R0010 on pSB1C3, BBa\_K912000 on pSB1C3

NanoDrop

Researcher: Hirao

Part: BBa\_K912001 \_ INSERT 5 on pSB1C3,
BBa\_R0010 on pSB1C3, BBa\_K912000 on pSB1C3

Colony PCR

Researcher: Hirao, Kono

Part: BBa\_K912001 \_ INSERT 5 on pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa\_K912001 \_ INSERT 5 on pSB1C3

Digestion

Researcher: Hirao, Kono

Part: BBa\_R0010 on pSB1C3, BBa\_K912000 on pSB1C3

Ethanol Precipitation

Researcher: Hirao

Part: BBa\_R0010 on pSB1C3, BBa\_K912000 on
pSB1C3

**10/12**

Gel Extraction

Researcher: Hirao

Part: BBa\_R0010, BBa\_K912000

NanoDrop

Researcher: Hirao

Part: BBa\_R0010, BBa\_K912000

Electrophoresis

Researcher: Hirao

Part: BBa\_K921001 \_ Part 5

Colony PCR

Researcher: Kono

Part: INSERT 1 \_ INSERT 2. INSERT 1 \_
INSERT 3, INSERT 1 \_ INSERT 4

**10/13**

Digestion

Researcher: Kono

Part: BBa\_K921000 on pSB1C3, BBa\_K912001 on
pSB1C3

Ethanol Precipitation

Researcher: Hirao

Part: BBa\_K921000, BBa\_K912001

Gel Extraction

Researcher: Hirao

Part: BBa\_K921000, BBa\_K912001

NanoDrop

Researcher: Hirao

Part: BBa\_K921000, BBa\_K912001

Electrophoresis

Researcher: Hirao

Part: BBa\_K921000, BBa\_K912001

Digestion

Researcher: Kono

Part: INSERT 1, INSERT 3, BBa\_K921000 on
pSB1C3, BBa\_K912001

Ligation

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 4

Electrophoresis

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 4

Ethanol Precipitation

Researcher: Kono

Part: INSERT 1, INSERT 3, BBa\_K912000,
BBa\_K912001

Gel Extract

Researcher: Hirao

Part: INSERT 1, INSERT 3, BBa\_K912000,
BBa\_K912001

**10/14**

NanoDrop

Researcher: Hirao

Part: INSERT 1, BBa\_K912000, BBa\_K912001

PCR

Researcher: Kono

Part: INSERT 3, INSERT 5

Ligation

Researcher: Kono

Part: INSERT 1 \_ INSERT 2, INSERT 1 \_
INSERT 4

Ligation

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5, BBa\_K912001 \_
INSERT 5

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 1 \_ INSERT 2 \_ pSB1C3, INSERT
1 \_ INSERT 4 \_ pSB1C3, BBa\_R0010, BBa\_K912000 \_ INSERT 5, BBa\_K912000,
BBa\_K912001 \_ INSERT 5, BBa\_K912001

Electrophoresis

Researcher: Hirao

Part: INSERT 3, INSERT 5

PCR Clean-up

Researcher: Hirao

Part: INSERT 3, INSERT 5

NanoDrop

Researcher: Hirao

Part: INSERT 3, INSERT 5

Digestion

Researcher: Hirao

Part: INSERT 3, INSERT 5

Transformation

Researcher: Hirao

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 4 on pSB1C3, BBa\_R0010

**10/15**

Ethanol Precipitation

Researcher: Kono

Part: INSERT 3, INSERT 5

Electrophoresis

Researcher: Kono

Part: INSERT 3, INSERT 5

Gel Extraction

Researcher: Kono

Part: INSERT 3, INSERT 5

NanoDrop

Researcher: Kono

Part: INSERT 3, INSERT 5

Transformation

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5 on pSB1C3,
BBa\_K912991 \_ INSERT 5 on pSB1C3

Ligation

Researcher: Kono

Part: INSERT 1 \_ INSERT 3 on pSB1C3

Colony PCR

Researcher: Kono

Part: INSERT 1- INSERT 2, INSERT 1 \_ INSERT
4

Digestion

Researcher: Kono

Part: BBa\_K912000 on pSB1C3, BBa\_K912001 on
pSB1C3

Colony PCR

Researcher: Kono

Part: INSERT 1 \_ INSERT 2, INSERT 1 \_
INSERT 4

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 1 \_ INSERT 3 on pSB1C3

Colony PCR

Researcher: Hirao

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 4 on pSB1C3

Transformation

Researcher: Hirao

Part: INSERT 1 \_ ISNERT 3 on pSB1C3

Ethanol Precipitation

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001

Electrophoresis

Researcher: Kono

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 4 on pSB1C3

**10/16**

Gel Extraction

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001

NanoDrop

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001

Ligation

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5 on pSB1C3, BBa\_K912001
\_ INSERT 5 on pSB1C3, BBa\_R0010 on pSB1C3

Colony PCR

Researcher: Kono

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 4 on pSB1C3

Electrophoresis

Researcher: Kono

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 4 on pSB1C3

Ethanol Precipitation

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5 on pSB1C3, BBa\_K912001
\_ INSERT 5 on pSB1C3, BBa\_R0010 on pSB1C3

Colony PCR

Researcher: Kono

Part: INSERT 1 \_ INSERT 3 on pSB1C3, BBa\_K912000
\_ INSERT 5 on pSB1C3, BBa\_K912001 \_ INSERT 5 on pSB1C3

Electrophoresis

Researcher: Kono

Part: INSERT 1 \_ INSERT 3 on pSB1C3, BBa\_K912000
\_ INSERT 5 on pSB1C3, INSERT 5

Digestion

Researcher: Kono

Part: BBa\_K592009 on pSB1C3

Liquid Culture

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5, BBa\_K592009
on pSB1C3, BBa\_K912001 on pSB1C3

**10/17**

Ethanol Precipitation

Researcher: Kono

Part: BBa\_K592009

Mini-Prep

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5 on pSB1C3,
BBa\_K592009 on pSB1C3, BBa\_K912001 on pSB1C3

NanoDrop

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5 on pSB1C3,
BBa\_K592009 on pSB1C3, BBa\_K912001 on pSB1C3

Electrophoresis

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5 on pSB1C3,
BBa\_K592009 on pSB1C3, BBa\_K912001 on pSB1C3

Digestion

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5 on pSB1C3,
BBa\_K912001 \_ INSERT 5 on pSB1C3

Electrophoresis

Researcher: Kishimoto

Part: BBa\_K912000 \_ INSERT 5, BBa\_K912001 \_
INSERT 5

Ethanol Precipitation

Researcher: Hirao

Part: BBa\_K912000 \_ INSERT 5, BBa\_K912001 \_
INSERT 5

**10/18**

PCR

Researcher: Hirao

Part: BBa\_K912000 \_ INSERT 5, BBa\_K912001 \_
INSERT 5

Gel Extraction

Researcher: Hirao

Part: BBa\_K912000 \_ INSERT 5

NanoDrop

Researcher: Hirao

Part: BBa\_K912000 \_ INSERT 5

Ligation

Researcher: Hirao

Part: BBa\_K912000 \_ INSERT 5

Electrophoresis

Researcher: Hirao

Part: BBa\_K912000 \_ INSERT 5

Ligation

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5

Ligation

Researcher: Kono

Part: INSERT 2, INSERT 3, INSERT 4

PCR

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5, BBa\_912001 \_
INSERT 5

Electrophoresis

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5, BBa\_912001 \_
INSERT 5

Ligation

Researcher: Kono

Part: INSERT 2 \_ pSB1C3, INSERT 3 \_ pSB1C3,
INSERT 4 \_ pSB1C3

**10/19**

Ethanol Precipitation

Researcher: Kono

Part: INSERT 2 \_ pSB1C3, INSERT 3 \_ pSB1C3,
INSERT 4 \_ pSB1C3

Gel Extraction

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5, BBa\_K912001 \_
INSERT 5

Transformation

Researcher: Kono,
Kamo

Part: INSERT 2 \_ pSB1C3, INSERT 3 \_ pSB1C3,
INSERT 4 \_ pSB1C3

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

**10/20**

PCR Clean-up

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

NanoDrop

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Digestion

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Colony PCR

Researcher: Hirao, Kono

Part: INSERT 2 on pSB1C3, INSERST 4 on
pSB1C3

Ethanol Precipitation

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Electrophoresis

Researcher: Kono

Part: INSERT 2 on pSB1C3, INSERST 4 on
pSB1C3

**10/21**

Transformation

Researcher: Kono

Part: INSERT 2 on pSB1C3, INSERST 4 on
pSB1C3

Liquid Culture

Researcher: Kono

Part: INSERT 2 on pSB1C3

Gel Extraction

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Ligation

Researcher: Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on
pSB1C3

Mini-Prep

Researcher: Kono

Part: INSERT 4 on pSB1C3

NanoDrop

Researcher: Kono

Part: INSERT 4 on pSB1C3

Ethanol Precipitation

Researcher: Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on pSB1C3

Colony PCR

Researcher: Hirao

Part: INSERT 2 on pSB1C3

Transformation

Researcher: Hirao, Kono

Part: INSERT 2 on pSB1C3, INSERT 4 on pSB1C3

Electrophoresis

Researcher: Hirao, Kono

Part: INSERT 4 on pSB1C3

Digestion

Researcher: Hirao

Part: INSERT 4 on pSB1C3

**10/22**

Electrophoresis

Researcher: Hirao

Part: INSERT 2 on pSB1C3, INSERT 4 on
pSB1C3

Liquid Culture

Researcher: Kono

Part: INSERT 2 on pSB1C3

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 4 on pSB1C3

Electrophoresis

Researcher: Hirao

Part: INSERT 2 on pSB1C3, INSERT 3 on
pSB1C3, INSERT 4

Gel Extraction

Researcher: Hirao

Part: INSERT 4 on pSB1C3

NanoDrop

Researcher: Hirao

Part: INSERT 4 on pSB1C3

Ligation

Researcher: Hirao

Part: INSERT 4 on pSB1C3

Electrophoresis

Researcher: Hirao

Part: INSERT 4 on pSB1C3

Colony PCR

Researcher: Hirao

Part: INSERT 2 on pSB1C3, INSERT 3 on
pSB1C3

Mini-Prep

Researcher: Hirao

Part: INSERT 2 on pSB1C3

NanoDrop

Researcher: Hirao

Part: INSERT 2 on pSB1C3

Digestion

Researcher: Hirao

Part: INSERT 2 on pSB1C3

Electrophoresis

Researcher: Hirao

Part: INSERT 2 on pSB1C3, INSERT 2

**10/23**

PCR

Researcher: Hirao

Part: INSERT 4

Electrophoresis

Researcher: Hirao

Part: INSERT 2 on pSB1C3, INSERT 3 on
pSB1C3

Colony PCR

Researcher: Hirao, Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on
pSB1C3

PCR

Researcher: Hirao, Kono

Part: INSERT 1, INSERT 2, INSERT 3

PCR Clean-up

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3

**10/24**

Ligation

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, pSB1C3

Electrophoresis

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, pSB1C3

PCR

Researcher: Kono

Part: INSERT 4

Digestion

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3

PCR Clean-up

Researcher: Hirao

Part: INSERT 4

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3

NanoDrop

Researcher: Hirao

Part: INSERT 4

Digestion

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, BBa\_K912000 \_ INSERT 5

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, BBa\_K912000 \_ INSERT 5

Gel Extraction

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, BBa\_K912000 \_ INSERT 5

Electrophoresis

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4, BBa\_K912000 \_ INSERT 5

Digestion

Researcher: Hirao

Part: BBa\_K912000 \_ INSERT 5

Mini-Prep

Researcher: Hirao

Part: BBa\_K912000 \_ INSERT 5

NanoDrop

Researcher: Hirao

Part: BBa\_K912000 \_ INSERT 5

Ethanol Precipitation

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5

**10/25**

Electrophoresis

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5

NanoDrop

Researcher: Kono

Part: BBa\_K912000 \_ INSERT 5

Ligation

Researcher: Kono

Part: INSERT 2 \_ pSB1C3, INSERT 3 \_ pSB1C3,
INSERT 4 \_ pSB1C3

Digestion

Researcher: Hirao

Part: BBa\_K912000 \_ INSERT 5

Ethanol Precipitation

Researcher: Hirao

Part: INSERT 2 \_ pSB1C3, INSERT 3 \_ pSB1C3,
INSERT 4 \_ pSB1C3, pSB1C3

Transformation

Researcher: Hirao

Part: INSERT 2 \_ pSB1C3, INSERT 3 \_ pSB1C3,
INSERT 4 \_ pSB1C3, pSB1C3

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Digestion

Researcher: Hirao

Part: BBa\_K912000 \_ INSERT 5

PCR Clean-up

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

NanoDrop

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Mini-Prep

Researcher: Kono

Part: BBa\_K912000, BBa\_K912001

Digestion

Researcher: Hirao

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Digestion

Researcher: Kono

Part: pSB1C3

Electrophoresis

Researcher: Hirao

Part: pSB1C3

Ethanol Precipitation

Researcher: Hirao

Part: pSB1C3

Gel Extraction

Researcher: Hirao

Part: pSB1C3

Ethanol Precipitation

Researcher: Kono

Part: pSB1C3

Electrophoresis

Researcher: Hirao

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3,
INSERT 4

Electrophoresis

Researcher: Kono

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3,
INSERT 4

**10/26**

Gel Extraction

Researcher: Kono

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3,
INSERT 4

NanoDrop

Researcher: Hirao

Part: pSB1C3, INSERT 1, INSERT 2, INSERT 3,
INSERT 4

Gel Extraction

Researcher: Kono

Part: pSB1C3, INSERT 2, INSERT 3, INSERT 4

NanoDrop

Researcher: Kono

Part: pSB1C3, INSERT 2, INSERT 3, INSERT 4

Ligation

Researcher: Hirao

Part: INSERT 1 on pSB1C3, INSERT 2 on
pSB1C3, INSERT 3 on pSB1C3, INSERT 4 on pSB1C3

Ligation

Researcher: Kono

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 3 on pSB1C3, INSERT 1 \_ INSERT 4 on pSB1C3

Ligation

Researcher: Kono

Part: INSERT 1 \_ pSB1C3, INSERT 2 \_ pSB1C3,
INSERT 3 \_ pSB1C3, INSERT 4 \_ pSB1C3

Ethanol Precipitation

Researcher: Hirao, Kono

Part: INSERT 1 on pSB1C3, INSERT 2 on
pSB1C3, INSERT 3 on pSB1C3, INSERT 4 on pSB1C3, INSERT 1 \_ INSERT 2 on pSB1C3,
INSERT 1 \_ INSERT 3 on pSB1C3, INSERT 1 \_ INSERT 4 on pSB1C3

Transformation

Researcher: Hirao, Kono

Part: INSERT 1 on pSB1C3, INSERT 2 on
pSB1C3, INSERT 3 on pSB1C3, INSERT 4 on pSB1C3, INSERT 1 \_ INSERT 2 on pSB1C3,
INSERT 1 \_ INSERT 3 on pSB1C3, INSERT 1 \_ INSERT 4 on pSB1C3

Colony PCR

Researcher: Hirao, Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on
pSB1C3, INSERT 4 on pSB1C3

Electrophoresis

Researcher: Kono

Part: INSERT 2 on pSB1C3, INSERT 3 on
pSB1C3, INSERT 4 on pSB1C3

**10/27**

Colony PCR

Researcher: Kono

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 3 on pSB1C3, INSERT 1 \_ INSERT 4 on pSB1C3

PCR

Researcher: Kono

Part: INSERT 1, INSERT 2, INSERT 3, INSERT
4

Colony PCR

Researcher: Kono

Part: INSERT 1 \_ INSERT 2 on pSB1C3, INSERT
1 \_ INSERT 3 on pSB1C3, INSERT 1 \_ INSERT 4 on pSB1C3

**10/28**

PCR

Researcher: Kono

Part: INSERT 1 on pSB1C3, INSERT 2 on
pSB1C3, INSERT 3 on pSB1C3, INSERT 4 on pSB1C3

NanoDrop

Researcher: Kono

Part: INSERT 1 on pSB1C3, INSERT 2 on
pSB1C3, INSERT 3 on pSB1C3, INSERT 4 on pSB1C3

**10/29**

Protein Extraction

Researcher: Hirao, Kono

Part: INSERT 2 on
pSB1C3, INSERT 3 on pSB1C3

**10/30**

Protein Extraction

Researcher: Hirao, Kono

Part: INSERT 2 on
pSB1C3, INSERT 3 on pSB1C3

**10/31**

Protein Extraction

Researcher: Hirao, Kono

Part: INSERT 2 on
pSB1C3, INSERT 3 on pSB1C3

**11/1**

Protein Concentration

Researcher: Hirao, Kono

Part: INSERT 2 on
pSB1C3, INSERT 3 on pSB1C3

Activity Measurement

Researcher: Hirao, Kono

Part: INSERT 2 on
pSB1C3, INSERT 3 on pSB1C3

SDS-PAGE

Researcher: Hirao, Kishimoto

Part: INSERT 2 on
pSB1C3, INSERT 3 on pSB1C3

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