iGEM 2008 — Team Calgary_Wetware

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# Team:Calgary Wetware/Notebook

### From 2008.igem.org

| [Home](/Team%3ACalgary_Wetware "Team:Calgary Wetware") | [The Team](/Team%3ACalgary_Wetware/Team "Team:Calgary Wetware/Team") | [The Project](/Team%3ACalgary_Wetware/Project "Team:Calgary Wetware/Project") | **Notebook** | [Protocols](/Team%3ACalgary_Wetware/Protocols "Team:Calgary Wetware/Protocols") | [Results](/Team%3ACalgary_Wetware/Results "Team:Calgary Wetware/Results") | [Beyond the Lab](/Team%3ACalgary_Wetware/Beyond "Team:Calgary Wetware/Beyond") | [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Calgary\_Wetware Parts] |
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## Notebook

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| June | | | | | | |
| M | T | W | T | F | S | S |
|  |  |  |  |  |  | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/1\_June\_2008&action=edit 1] |
| [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/2\_June\_2008&action=edit 2] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/3\_June\_2008&action=edit 3] | [http://2008.igem.org/Team:Calgary\_Wetware/4\_June\_2008 4] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/5\_June\_2008&action=edit 5] | [http://2008.igem.org/Team:Calgary\_Wetware/6\_June\_2008 6] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/7\_June\_2008&action=edit 7] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/8\_June\_2008&action=edit 8] |
| [http://2008.igem.org/Team:Calgary\_Wetware/9\_June\_2008 9] | [http://2008.igem.org/Team:Calgary\_Wetware/10\_June\_2008 10] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/11\_June\_2008&action=edit 11] | [http://2008.igem.org/Team:Calgary\_Wetware/12\_June\_2008 12] | [http://2008.igem.org/Team:Calgary\_Wetware/13\_June\_2008 13] | [http://2008.igem.org/Team:Calgary\_Wetware/14\_June\_2008 14] | [http://2008.igem.org/Team:Calgary\_Wetware/15\_June\_2008 15] |
| [http://2008.igem.org/Team:Calgary\_Wetware/16\_June\_2008 16] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/17\_June\_2008&action=edit 17] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/18\_June\_2008&action=edit 18] | [http://2008.igem.org/Team:Calgary\_Wetware/19\_June\_2008 19] | [http://2008.igem.org/Team:Calgary\_Wetware/20\_June\_2008 20] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/21\_June\_2008&action=edit 21] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/22\_June\_2008&action=edit 22] |
| [http://2008.igem.org/Team:Calgary\_Wetware/23\_June\_2008 23] | [http://2008.igem.org/Team:Calgary\_Wetware/24\_June\_2008 24] | [http://2008.igem.org/Team:Calgary\_Wetware/25\_June\_2008 25] | [http://2008.igem.org/Team:Calgary\_Wetware/26\_June\_2008 26] | [http://2008.igem.org/Team:Calgary\_Wetware/27\_June\_2008 27] | [http://2008.igem.org/Team:Calgary\_Wetware/28\_June\_2008 28] | [http://2008.igem.org/Team:Calgary\_Wetware/29\_June\_2008 29] |
| [http://2008.igem.org/Team:Calgary\_Wetware/30\_June\_2008 30] |

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| July | | | | | | |
| M | T | W | T | F | S | S |
|  | [http://2008.igem.org/Team:Calgary\_Wetware/1\_July\_2008 1] | [http://2008.igem.org/Team:Calgary\_Wetware/2\_July\_2008 2] | [http://2008.igem.org/Team:Calgary\_Wetware/3\_July\_2008 3] | [http://2008.igem.org/Team:Calgary\_Wetware/4\_July\_2008 4] | [http://2008.igem.org/Team:Calgary\_Wetware/5\_July\_2008 5] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/6\_July\_2008&action=edit 6] |
| [http://2008.igem.org/Team:Calgary\_Wetware/7\_July\_2008 7] | [http://2008.igem.org/Team:Calgary\_Wetware/8\_July\_2008 8] | [http://2008.igem.org/Team:Calgary\_Wetware/9\_July\_2008 9] | [http://2008.igem.org/Team:Calgary\_Wetware/10\_July\_2008 10] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/11\_July\_2008&action=edit 11] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/12\_July\_2008&action=edit 12] | [http://2008.igem.org/Team:Calgary\_Wetware/13\_July\_2008 13] |
| [http://2008.igem.org/Team:Calgary\_Wetware/14\_July\_2008 14] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/15\_July\_2008&action=edit 15] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/16\_July\_2008&action=edit 16] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/17\_July\_2008&action=edit 17] | [http://2008.igem.org/Team:Calgary\_Wetware/18\_July\_2008 18] | [http://2008.igem.org/Team:Calgary\_Wetware/19\_July\_2008 19] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/20\_July\_2008&action=edit 20] |
| [http://2008.igem.org/Team:Calgary\_Wetware/21\_July\_2008 21] | [http://2008.igem.org/Team:Calgary\_Wetware/22\_July\_2008 22] | [http://2008.igem.org/Team:Calgary\_Wetware/23\_July\_2008 23] | [http://2008.igem.org/Team:Calgary\_Wetware/24\_July\_2008 24] | [http://2008.igem.org/Team:Calgary\_Wetware/25\_July\_2008 25] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/26\_July\_2008&action=edit 26] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/27\_July\_2008&action=edit 27] |
| [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/28\_July\_2008&action=edit 28] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/29\_July\_2008&action=edit 29] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/30\_July\_2008&action=edit 30] | [http://2008.igem.org/Team:Calgary\_Wetware/31\_July\_2008 31] |

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| August | | | | | | |
| M | T | W | T | F | S | S |
|  |  |  |  | [http://2008.igem.org/Team:Calgary\_Wetware/1\_August\_2008 1] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/2\_August\_2008&action=edit 2] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/3\_August\_2008&action=edit 3] |
| [http://2008.igem.org/Team:Calgary\_Wetware/4\_August\_2008 4] | [http://2008.igem.org/Team:Calgary\_Wetware/5\_August\_2008 5] | [http://2008.igem.org/Team:Calgary\_Wetware/6\_August\_2008 6] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/7\_August\_2008&action=edit 7] | [http://2008.igem.org/Team:Calgary\_Wetware/8\_August\_2008 8] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/9\_August\_2008&action=edit 9] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/10\_August\_2008&action=edit 10] |
| [http://2008.igem.org/Team:Calgary\_Wetware/11\_August\_2008 11] | [http://2008.igem.org/Team:Calgary\_Wetware/12\_August\_2008 12] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/13\_August\_2008&action=edit 13] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/14\_August\_2008&action=edit 14] | [http://2008.igem.org/Team:Calgary\_Wetware/15\_August\_2008 15] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/16\_August\_2008&action=edit 16] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/17\_August\_2008&action=edit 17] |
| [http://2008.igem.org/Team:Calgary\_Wetware/18\_August\_2008 18] | [http://2008.igem.org/Team:Calgary\_Wetware/19\_August\_2008 19] | [http://2008.igem.org/Team:Calgary\_Wetware/20\_August\_2008 20] | [http://2008.igem.org/Team:Calgary\_Wetware/21\_August\_2008 21] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/22\_August\_2008&action=edit 22] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/23\_August\_2008&action=edit 23] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/24\_August\_2008&action=edit 24] |
| [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/25\_August\_2008&action=edit 25] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/26\_August\_2008&action=edit 26] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/27\_August\_2008&action=edit 27] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/28\_August\_2008&action=edit 28] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/29\_August\_2008&action=edit 29] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/30\_August\_2008&action=edit 30] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/31\_August\_2008&action=edit 31] |

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| September | | | | | | |
| M | T | W | T | F | S | S |
| [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/1\_September\_2008&action=edit 1] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/2\_September\_2008&action=edit 2] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/3\_September\_2008&action=edit 3] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/4\_September\_2008&action=edit 4] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/5\_September\_2008&action=edit 5] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/6\_September\_2008&action=edit 6] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/7\_September\_2008&action=edit 7] |
| [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/8\_September\_2008&action=edit 8] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/9\_September\_2008&action=edit 9] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/10\_September\_2008&action=edit 10] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/11\_September\_2008&action=edit 11] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/12\_September\_2008&action=edit 12] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/13\_September\_2008&action=edit 13] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/14\_September\_2008&action=edit 14] |
| [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/15\_September\_2008&action=edit 15] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/16\_September\_2008&action=edit 16] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/17\_September\_2008&action=edit 17] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/18\_September\_2008&action=edit 18] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/19\_September\_2008&action=edit 19] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/20\_September\_2008&action=edit 20] | [http://2008.igem.org/Team:Calgary\_Wetware/21\_September\_2008 21] |
| [http://2008.igem.org/Team:Calgary\_Wetware/22\_September\_2008 22] | [http://2008.igem.org/Team:Calgary\_Wetware/23\_September\_2008 23] | [http://2008.igem.org/Team:Calgary\_Wetware/24\_September\_2008 24] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/25\_September\_2008&action=edit 25] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/26\_September\_2008&action=edit 26] | [http://2008.igem.org/Team:Calgary\_Wetware/27\_September\_2008 27] | [http://2008.igem.org/Team:Calgary\_Wetware/28\_September\_2008 28] |
| [http://2008.igem.org/Team:Calgary\_Wetware/29\_September\_2008 29] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/30\_September\_2008&action=edit 30] |

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| October | | | | | | |
| M | T | W | T | F | S | S |
|  |  | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/1\_October\_2008&action=edit 1] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/2\_October\_2008&action=edit 2] | [http://2008.igem.org/Team:Calgary\_Wetware/3\_October\_2008 3] | [http://2008.igem.org/Team:Calgary\_Wetware/4\_October\_2008 4] | [http://2008.igem.org/Team:Calgary\_Wetware/5\_October\_2008 5] |
| [http://2008.igem.org/Team:Calgary\_Wetware/6\_October\_2008 6] | [http://2008.igem.org/Team:Calgary\_Wetware/7\_October\_2008 7] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/8\_October\_2008&action=edit 8] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/9\_October\_2008&action=edit 9] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/10\_October\_2008&action=edit 10] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/11\_October\_2008&action=edit 11] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/12\_October\_2008&action=edit 12] |
| [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/13\_October\_2008&action=edit 13] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/14\_October\_2008&action=edit 14] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/15\_October\_2008&action=edit 15] | [http://2008.igem.org/Team:Calgary\_Wetware/16\_October\_2008 16] | [http://2008.igem.org/Team:Calgary\_Wetware/17\_October\_2008 17] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/18\_October\_2008&action=edit 18] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/19\_October\_2008&action=edit 19] |
| [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/20\_October\_2008&action=edit 20] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/21\_October\_2008&action=edit 21] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/22\_October\_2008&action=edit 22] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/23\_October\_2008&action=edit 23] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/24\_October\_2008&action=edit 24] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/25\_October\_2008&action=edit 25] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/26\_October\_2008&action=edit 26] |
| [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/27\_October\_2008&action=edit 27] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/28\_October\_2008&action=edit 28] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/29\_October\_2008&action=edit 29] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/30\_October\_2008&action=edit 30] | [http://2008.igem.org/wiki/index.php?title=Team:Calgary\_Wetware/31\_October\_2008&action=edit 31] |

## Other

* PCR conditions for amplifying the ColE2 operon.

[![PCRs.jpg](/wiki/images/f/f5/PCRs.jpg)](/File%3APCRs.jpg)

* The primers I used in my experiments:

[![Primers.jpg](/wiki/images/e/e7/Primers.jpg)](/File%3APrimers.jpg)

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