iGEM 2017 — Team Cologne-Duesseldorf

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Cologne-Duesseldorf/Notebook

* [Home](https://2017.igem.org/Team%3ACologne-Duesseldorf)

[Team](#!)+ [Team](https://2017.igem.org/Team%3ACologne-Duesseldorf/Team)
+ [Collaborations](https://2017.igem.org/Team%3ACologne-Duesseldorf/Collaborations)

[Project](#!)+ [Description](https://2017.igem.org/Team%3ACologne-Duesseldorf/Description)
+ [Design](https://2017.igem.org/Team%3ACologne-Duesseldorf/Design)
+ [Experiments](https://2017.igem.org/Team%3ACologne-Duesseldorf/Experiments)
+ [Notebook](https://2017.igem.org/Team%3ACologne-Duesseldorf/Notebook)
+ [InterLab](https://2017.igem.org/Team%3ACologne-Duesseldorf/InterLab)
+ [Model](https://2017.igem.org/Team%3ACologne-Duesseldorf/Model)
+ [Results](https://2017.igem.org/Team%3ACologne-Duesseldorf/Results)
+ [Demonstrate](https://2017.igem.org/Team%3ACologne-Duesseldorf/Demonstrate)
+ [Improve](https://2017.igem.org/Team%3ACologne-Duesseldorf/Improve)
+ [Attributions](https://2017.igem.org/Team%3ACologne-Duesseldorf/Attributions)

[Parts](#!)+ [Parts](https://2017.igem.org/Team%3ACologne-Duesseldorf/Parts)
+ [Basic Parts](https://2017.igem.org/Team%3ACologne-Duesseldorf/Basic_Part)

* [Safety](https://2017.igem.org/Team%3ACologne-Duesseldorf/Safety)

[Human Practices](#!)+ [Silver](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Silver)
+ [Integrated Practices](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Gold_Integrated)
+ [Public Engagement](https://2017.igem.org/Team%3ACologne-Duesseldorf/Engagement)

[Awards](#!)+ [Applied Design](https://2017.igem.org/Team%3ACologne-Duesseldorf/Applied_Design)
+ [Entrepreneurship](https://2017.igem.org/Team%3ACologne-Duesseldorf/Entrepreneurship)
+ [Hardware](https://2017.igem.org/Team%3ACologne-Duesseldorf/Hardware)
+ [Model](https://2017.igem.org/Team%3ACologne-Duesseldorf/Model)
+ [Plant](https://2017.igem.org/Team%3ACologne-Duesseldorf/Plant)
+ [Software](https://2017.igem.org/Team%3ACologne-Duesseldorf/Software)

* [Judging Form](https://igem.org/2017_Judging_Form?team=Cologne-Duesseldorf)
* [Wiki Tutorial](https://2017.igem.org/Team%3ACologne-Duesseldorf/Wiki-Tutorial)

* [![](https://static.igem.org/mediawiki/2017/1/18/T--Cologne-Duesseldorf--artico-logo-base.svg)](https://2017.igem.org/Team%3ACologne-Duesseldorf)
* [Team](#!)
  + [Team](https://2017.igem.org/Team%3ACologne-Duesseldorf/Team)
  + [Collaborations](https://2017.igem.org/Team%3ACologne-Duesseldorf/Collaborations)
* [Project](#!)
  + [Description](https://2017.igem.org/Team%3ACologne-Duesseldorf/Description)
  + [Design](https://2017.igem.org/Team%3ACologne-Duesseldorf/Design)
  + [Experiments](https://2017.igem.org/Team%3ACologne-Duesseldorf/Experiments)
  + [Notebook](https://2017.igem.org/Team%3ACologne-Duesseldorf/Notebook)
  + [InterLab](https://2017.igem.org/Team%3ACologne-Duesseldorf/InterLab)
  + [Model](https://2017.igem.org/Team%3ACologne-Duesseldorf/Model)
  + [Results](https://2017.igem.org/Team%3ACologne-Duesseldorf/Results)
  + [Demonstrate](https://2017.igem.org/Team%3ACologne-Duesseldorf/Demonstrate)
  + [Improve](https://2017.igem.org/Team%3ACologne-Duesseldorf/Improve)
  + [Attributions](https://2017.igem.org/Team%3ACologne-Duesseldorf/Attributions)
* [Parts](#!)
  + [Parts](https://2017.igem.org/Team%3ACologne-Duesseldorf/Parts)
  + [Basic Parts](https://2017.igem.org/Team%3ACologne-Duesseldorf/Basic_Part)
* [Safety](https://2017.igem.org/Team%3ACologne-Duesseldorf/Safety)
* [Human Practices](#!)
  + [Silver](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Silver)
  + [Integrated Practices](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Gold_Integrated)
  + [Public Engagement](https://2017.igem.org/Team%3ACologne-Duesseldorf/Engagement)
* [Awards](#!)
  + [Applied Design](https://2017.igem.org/Team%3ACologne-Duesseldorf/Applied_Design)
  + [Hardware](https://2017.igem.org/Team%3ACologne-Duesseldorf/Hardware)
  + [Model](https://2017.igem.org/Team%3ACologne-Duesseldorf/Model)
  + [Software](https://2017.igem.org/Team%3ACologne-Duesseldorf/Software)
* [Judging Form](https://igem.org/2017_Judging_Form?team=Cologne-Duesseldorf)
* [Wiki Tutorial](https://2017.igem.org/Team%3ACologne-Duesseldorf/Wiki-Tutorial)

# Notebook

## Weekly meetings

* 16.01.17-22.01.17:

+ Presentation of iGEM at the department of biology at the Heinrich-Heine-University, Duesseldorf
+ Tour around our new workspace in the lab of Prof. Dr. Pauly

* 23.01.17-29.01.17

+ Presentation of iGEM in the University of Cologne to find new members and establish a collaboration between the two universities

* 30.01.17-05.02.17

+ Team selection and first get together of the whole [team](https://2017.igem.org/Team%3ACologne-Duesseldorf/Team)
+ First brainstorming with members from the University of Cologne and the Heinrich-Heine-University Duesseldorf

* 06.02.17-12.02.17

+ Second brainstorming at the weekend with the whole team and teambuilding

* 13.02.17-19.02.17

+ Received material donations from Starlab
+ Received material donations from Biozym

* 20.02.17-26.02.17

+ Presenting our first project ideas to different professors from our universities, Prof. Dr. Andreas Weber, Prof. Dr. Zurbriggen, Prof. Dr. Axmann as well as Danny Ducat from Michigan State University, US
+ Received material donations from Carl Roth GmbH + Co. KG

* 06.03.17-12.03.17

+ Team registration
+ Teambuilding weekend in Aachen and decision on [our project: Artificial compartments](https://2017.igem.org/Team%3ACologne-Duesseldorf/Description)
+ Received donation from Bayer AG

* 13.03.17-19.03.17

+ Safety instructions for working in our lab at the Institute from Prof. Dr. Pauly

* 27.03.17-02.04.17

+ Chose our team [leader](https://2017.igem.org/Team%3ACologne-Duesseldorf/Team#Rene-Inckemann)

* 03.04.17-09.04.17

+ Our registration has officially been accepted. iGEM 2017, here we come!
+ [Meeting](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Gold_Integrated#FoundationalKnowledge) with Prof. Dr. Ralf Erdmann
+ Presenting our project at CEPLAS Friday

* 10.04.17-16.04.17

+ Meeting with Brian Caudill from IDT to learn more about the free synthesis

* 17.04.17-23.04.17

+ Meeting a few members of Team Aachen, Bielefeld and Bonn at the [March of Science](https://2017.igem.org/Team%3ACologne-Duesseldorf/Engagement#h2-1) in Bonn

* 01.05.17-07.05.17

+ Received the iGEM Distribution kit
+ Presentation of our project at BioRiver, followed by consulting

* 15.05.17-21.05.17

+ Meeting with Team Bielefeld
+ Attending the [Labvolution](https://2017.igem.org/Team%3ACologne-Duesseldorf/Team#h2-5) at Hannover
+ Skype meeting with Sebastian Schulz, Marco Gierhard

* 22.05.17-28.05.17

+ Getting our [postcard campaign](https://2017.igem.org/Team%3ACologne-Duesseldorf/Engagement#Postcards) started

* 29.05.17-04.06.17

+ Meeting with Prof. Dr. Ralf Erdmann from the university of Bochum: Discussion about our project as he is an expert regarding peroxisomes

* 05.06.17-11.06.17

+ Get together and [camping](https://2017.igem.org/Team%3ACologne-Duesseldorf/Team#MeetUps) with Team Aachen

* 12.06.17-18.06.17

+ Attending the [SB7.0 Conference in Singapore](https://2017.igem.org/Team%3ACologne-Duesseldorf/Team#h2-4) and organizing a small iGEM meet up to present our project

* 19.06.17-25.06.17

+ Starting out [postcard campaign](https://2017.igem.org/Team%3ACologne-Duesseldorf/Engagement#Postcards)

* 26.06.17-02.07.17

+ Our first [lesson](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Silver#h2-2) with pupils from the "Leonardo da Vinci Gymnasium"
+ Presentation of our project at the ceremony of the [Karl von Frisch award](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Silver#h2-3)
+ Attendance of the [German Meetup](https://2017.igem.org/Team%3ACologne-Duesseldorf/Team#MeetUps) in Dresden

* 03.07.17-09.07.17

+ Attendance of the [European Meetup](https://2017.igem.org/Team%3ACologne-Duesseldorf/Team#MeetUps) in Delft

* 10.07.17-16.07.17

+ Thank you-Barbeque for the Institute of Prof. Dr. Pauly for being able to work in his lab
+ Meetup with Team Bielefeld

* 17.07.17-23.07.17

+ Winning the GenScript gold-level scholarship

* 24.07.17-30.07.17

+ Visited Dr. Johann Ach at the centre for [bioethics](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Gold_Integrated#Society) in Münster for a discussion about ethical and normative aspects of synthetic biology

* 31.07.17-06.08.17

+ [Skype meeting](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Gold_Integrated#PotentialApplications) with Florian David, Biopetrolia

* 07.08.17-13.08.17

+ [Skype meeting](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Gold_Integrated#PotentialApplications) with Mary Bossermann, Evolva

* 28.08.17-03.09.17

+ Thank you- Barbeque for the Institute of Prof. Dr. Döhlemann
+ [Optogenetics seminar](https://2017.igem.org/Team%3ACologne-Duesseldorf/Collaborations#h2-2) with Team Darmstadt and Team Heidelberg attending in person and Team Taiwan and Team UPMC Paris
+ [Local meetup](https://2017.igem.org/Team%3ACologne-Duesseldorf/Team#love) with Team Aachen, Team Bielefeld, Team Darmstadt and Team Heidelberg

* 04.09.17-10.09.17

+ Visit from Mrs. Wirth from Molecular Devices LLC

* 18.09.17-24.09.17

+ [iGEM Spirit](https://2017.igem.org/Team%3ACologne-Duesseldorf/Engagement#iGEMSpirit): Presenting our project in the Oldtown of Duesseldorf
+ Getting started on our wiki: First wiki weekend

* 25.09.17-01.10.17

+ Attending the [Jülich Biotech Day](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Silver#h2-5)
+ [Skype meeting](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Gold_Integrated#FoundationalKnowledge) with John Dueber and members of his lab, UC Berkeley
+ Skype meeting with Will DeLoache

* 02.10.17-08.10.17

+ First [SynBio Day](https://2017.igem.org/Team%3ACologne-Duesseldorf/Engagement#Synbio) organized by Team Cologne-Duesseldorf

* 16.10.17-22.10.17

+ Meeting with [Evolva at Basel](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Gold_Integrated#FoundationalKnowledge)
+ [Skype meeting](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Gold_Integrated#FoundationalKnowledge) with Evolva, USA

* 23.10.17-29.10.17

+ DNA Submission
+ [Judging Form](https://igem.org/2017_Judging_Form?team=Cologne-Duesseldorf)

* 30.10.17-05.11.17

+ [Final sprint](https://2017.igem.org/Team%3ACologne-Duesseldorf/Results) in the lab and working on our [wiki](https://2017.igem.org/Team%3ACologne-Duesseldorf)
+ Wikifreeze

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)


[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Cologne-Duesseldorf/Experiments

* [Home](https://2017.igem.org/Team%3ACologne-Duesseldorf)

[Team](#!)+ [Team](https://2017.igem.org/Team%3ACologne-Duesseldorf/Team)
+ [Collaborations](https://2017.igem.org/Team%3ACologne-Duesseldorf/Collaborations)

[Project](#!)+ [Description](https://2017.igem.org/Team%3ACologne-Duesseldorf/Description)
+ [Design](https://2017.igem.org/Team%3ACologne-Duesseldorf/Design)
+ [Experiments](https://2017.igem.org/Team%3ACologne-Duesseldorf/Experiments)
+ [Notebook](https://2017.igem.org/Team%3ACologne-Duesseldorf/Notebook)
+ [InterLab](https://2017.igem.org/Team%3ACologne-Duesseldorf/InterLab)
+ [Model](https://2017.igem.org/Team%3ACologne-Duesseldorf/Model)
+ [Results](https://2017.igem.org/Team%3ACologne-Duesseldorf/Results)
+ [Demonstrate](https://2017.igem.org/Team%3ACologne-Duesseldorf/Demonstrate)
+ [Improve](https://2017.igem.org/Team%3ACologne-Duesseldorf/Improve)
+ [Attributions](https://2017.igem.org/Team%3ACologne-Duesseldorf/Attributions)

[Parts](#!)+ [Parts](https://2017.igem.org/Team%3ACologne-Duesseldorf/Parts)
+ [Basic Parts](https://2017.igem.org/Team%3ACologne-Duesseldorf/Basic_Part)

* [Safety](https://2017.igem.org/Team%3ACologne-Duesseldorf/Safety)

[Human Practices](#!)+ [Silver](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Silver)
+ [Integrated Practices](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Gold_Integrated)
+ [Public Engagement](https://2017.igem.org/Team%3ACologne-Duesseldorf/Engagement)

[Awards](#!)+ [Applied Design](https://2017.igem.org/Team%3ACologne-Duesseldorf/Applied_Design)
+ [Entrepreneurship](https://2017.igem.org/Team%3ACologne-Duesseldorf/Entrepreneurship)
+ [Hardware](https://2017.igem.org/Team%3ACologne-Duesseldorf/Hardware)
+ [Model](https://2017.igem.org/Team%3ACologne-Duesseldorf/Model)
+ [Plant](https://2017.igem.org/Team%3ACologne-Duesseldorf/Plant)
+ [Software](https://2017.igem.org/Team%3ACologne-Duesseldorf/Software)

* [Judging Form](https://igem.org/2017_Judging_Form?team=Cologne-Duesseldorf)
* [Wiki Tutorial](https://2017.igem.org/Team%3ACologne-Duesseldorf/Wiki-Tutorial)

* [![](https://static.igem.org/mediawiki/2017/1/18/T--Cologne-Duesseldorf--artico-logo-base.svg)](https://2017.igem.org/Team%3ACologne-Duesseldorf)
* [Team](#!)
  + [Team](https://2017.igem.org/Team%3ACologne-Duesseldorf/Team)
  + [Collaborations](https://2017.igem.org/Team%3ACologne-Duesseldorf/Collaborations)
* [Project](#!)
  + [Description](https://2017.igem.org/Team%3ACologne-Duesseldorf/Description)
  + [Design](https://2017.igem.org/Team%3ACologne-Duesseldorf/Design)
  + [Experiments](https://2017.igem.org/Team%3ACologne-Duesseldorf/Experiments)
  + [Notebook](https://2017.igem.org/Team%3ACologne-Duesseldorf/Notebook)
  + [InterLab](https://2017.igem.org/Team%3ACologne-Duesseldorf/InterLab)
  + [Model](https://2017.igem.org/Team%3ACologne-Duesseldorf/Model)
  + [Results](https://2017.igem.org/Team%3ACologne-Duesseldorf/Results)
  + [Demonstrate](https://2017.igem.org/Team%3ACologne-Duesseldorf/Demonstrate)
  + [Improve](https://2017.igem.org/Team%3ACologne-Duesseldorf/Improve)
  + [Attributions](https://2017.igem.org/Team%3ACologne-Duesseldorf/Attributions)
* [Parts](#!)
  + [Parts](https://2017.igem.org/Team%3ACologne-Duesseldorf/Parts)
  + [Basic Parts](https://2017.igem.org/Team%3ACologne-Duesseldorf/Basic_Part)
* [Safety](https://2017.igem.org/Team%3ACologne-Duesseldorf/Safety)
* [Human Practices](#!)
  + [Silver](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Silver)
  + [Integrated Practices](https://2017.igem.org/Team%3ACologne-Duesseldorf/HP/Gold_Integrated)
  + [Public Engagement](https://2017.igem.org/Team%3ACologne-Duesseldorf/Engagement)
* [Awards](#!)
  + [Applied Design](https://2017.igem.org/Team%3ACologne-Duesseldorf/Applied_Design)
  + [Hardware](https://2017.igem.org/Team%3ACologne-Duesseldorf/Hardware)
  + [Model](https://2017.igem.org/Team%3ACologne-Duesseldorf/Model)
  + [Software](https://2017.igem.org/Team%3ACologne-Duesseldorf/Software)
* [Judging Form](https://igem.org/2017_Judging_Form?team=Cologne-Duesseldorf)
* [Wiki Tutorial](https://2017.igem.org/Team%3ACologne-Duesseldorf/Wiki-Tutorial)

# Experiments

## Protocols

![](https://static.igem.org/mediawiki/2017/f/f5/T--Cologne-Duesseldorf--StockExperiment.jpg)

* [PCR gel Cleanup](https://static.igem.org/mediawiki/2017/c/ce/T--Cologne-Duesseldorf--protocols-pcr-gel-cleanup.pdf)
* [Transformation](https://static.igem.org/mediawiki/2017/8/80/T--Cologne-Duesseldorf--protocols-transformation.pdf)
* [Test restriction digest](https://static.igem.org/mediawiki/2017/7/73/T--Cologne-Duesseldorf--protocols-test-restriction-digest.pdf)
* [Restriction digest](https://static.igem.org/mediawiki/2017/7/73/T--Cologne-Duesseldorf--protocols-restriction-digest.pdf)
* [Miniprep](https://static.igem.org/mediawiki/2017/7/7b/T--Cologne-Duesseldorf--protocols-miniprep-promega.pdf)
* [PCR](https://static.igem.org/mediawiki/2017/7/70/T--Cologne-Duesseldorf--protocols-pcr-phusion.pdf)
* [Colony PCR](https://static.igem.org/mediawiki/2017/8/87/T--Cologne-Duesseldorf--protocols-colony-pcr.pdf)
* [PCR purification](https://static.igem.org/mediawiki/2017/8/87/T--Cologne-Duesseldorf--protocols-pcr-purification-promega.pdf)
* [Yeast transformation](https://static.igem.org/mediawiki/2017/8/81/T--Cologne-Duesseldorf--yeast_transformation.pdf)
* [Western blot protocol](https://static.igem.org/mediawiki/2017/8/8a/T--Cologne-Duesseldorf--western-blot-protocol.pdf)
* [*In vitro* assay prodeoxyviolacein](https://static.igem.org/mediawiki/2017/a/aa/T--Cologne-Duesseldorf--prodeoxyviolacein_assay.pdf)

## Sub projects

* [Labbook − Team PEX5 import](https://static.igem.org/mediawiki/2017/7/74/Artico_labbook_MJ.pdf)
* [Labbook − Team PTS2 site directed mutagenesis](https://static.igem.org/mediawiki/2017/9/98/T--cologne-duesseldorf--Labbook_sitedirected_mutagenesis.pdf%20)
* [Labbook − Team PTS2 random mutagenesis](https://static.igem.org/mediawiki/2017/3/30/T--cologne-duesseldorf--labbook_PTS2_random_mutagenesis.pdf%20)
* [Labbook − Team Sensors](https://static.igem.org/mediawiki/2017/7/72/Artico_labbook.pdf)
* [Labbook − Team Secretion](https://static.igem.org/mediawiki/2017/1/14/T--Cologne-Duesseldorf--Secretionlabbook2.pdf)
* [Labbook − Team Membrane Integration](https://static.igem.org/mediawiki/2017/7/71/PMP-Labbook.pdf)
* [Labbook − Size and Number](https://static.igem.org/mediawiki/2017/5/5d/T--Cologne-Duesseldorf--labbook-size-and-number.pdf)
* [Labbook − Team Violacein](https://static.igem.org/mediawiki/parts/9/95/T--Cologne-Duesseldorf--labbook_Violacein.docx.pdf)
* [Labbook − Team Nootkatone](https://static.igem.org/mediawiki/parts/7/79/Labbook_Nootkatone.pdf)
* [Labbook − InterLab](https://static.igem.org/mediawiki/2017/d/d1/T--Cologne-Duesseldorf--Labbook_for_Interlab_study.pdf)
* [Labbook − Aachen Collaboration](https://static.igem.org/mediawiki/2017/a/ab/T--Cologne-Duesseldorf--Labbook_for_Aachen_cloning.pdf)

![](https://static.igem.org/mediawiki/2017/1/17/T--Cologne-Duesseldorf--m2.jpeg)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)