iGEM 2014 — Team HokkaidoU_Japan

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# Team:HokkaidoU Japan/Notebook

### From 2014.igem.org

![](https://static.igem.org/mediawiki/2014/b/bb/HokkaidoU_Header_Top.png)

Extra Materials

Notebook

![](https://static.igem.org/mediawiki/2014/4/44/HokkaidoU_Header_ki.png)

* [Top](https://2014.igem.org/Team%3AHokkaidoU_Japan)
* RNA in Love

  + [Overview](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/Overview/Background)
  + [Background](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/Overview/Background)
  + [Achievements](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/Overview/Achievements)
  + [H-stem System](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/H_Stem)
  + [Overview](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/H_Stem#Overview)
  + [Stabilization](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/H_Stem#asRNA_stabilization)
  + [Modelling](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/H_Stem#Modelling)
  + [Construct](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/H_Stem#Construct)
  + [How To Use](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/H_Stem#How_To_Use)
  + [Anti-sense B0034](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/asB0034)
  + [Overview](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/asB0034)
  + [Method](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/asB0034#Method)
  + [Results](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/asB0034#Results)
  + [Conclusion](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/asB0034#Conclusion)
  + [Length Variation](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/Length)
  + [Overview](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/Length)
  + [Method](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/Length#Method)
  + [Results](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/Length#Results)
  + [Conclusion](https://2014.igem.org/Team%3AHokkaidoU_Japan/Projects/Length#Conclusion)
  + [Extra Materials](https://2014.igem.org/Team%3AHokkaidoU_Japan/Materials)
  + [Parts](https://2014.igem.org/Team%3AHokkaidoU_Japan/Parts)
  + [Notebook](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook)
  + [Safety](https://2014.igem.org/Team%3AHokkaidoU_Japan/Safety)
* Outreach

  + [Univ. Festival](https://2014.igem.org/Team%3AHokkaidoU_Japan/Outreach/Univ_festival)
  + [Education](https://2014.igem.org/Team%3AHokkaidoU_Japan/Outreach/Univ_festival#Education)
  + [Survey](https://2014.igem.org/Team%3AHokkaidoU_Japan/Outreach/Univ_festival#Survey)
  + [High School](https://2014.igem.org/Team%3AHokkaidoU_Japan/Outreach/Survey)
  + [Education](https://2014.igem.org/Team%3AHokkaidoU_Japan/Outreach/Survey#Education)
  + [Survey](https://2014.igem.org/Team%3AHokkaidoU_Japan/Outreach/Survey#Survey)
  + [Discussion](https://2014.igem.org/Team%3AHokkaidoU_Japan/Outreach/Discussion)
  + [Background](https://2014.igem.org/Team%3AHokkaidoU_Japan/Outreach/Discussion#Background)
  + [Evaluation](https://2014.igem.org/Team%3AHokkaidoU_Japan/Outreach/Discussion#Evaluation)
* About Us

  + [About Us](https://2014.igem.org/Team%3AHokkaidoU_Japan/About_Us)
  + [Acknowledgements](https://2014.igem.org/Team%3AHokkaidoU_Japan/About_Us#Acknowledgements)
  + [Team](https://2014.igem.org/Team%3AHokkaidoU_Japan/About_Us#Team)

# Protocols

*[Protocols](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/Protocols)*

# Lab Documents

### Preliminary Experiment

*[Anti-Sense mRFP Construction](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/Pre_experiment/asmRFP)*

*[mRFP Expression Plasmid construction](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/Pre_experiment/mRFP-expression-construct)*

*[asB0034(pHN1257) Construction](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/asB0034/asB0034-construction)*

*[Ptet-B0032-mRFP-dT](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/Pre_experiment/140910_Ptet-B0032-mRFP-dT)*

*[Wrong Plac failed Experiment](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/Pre_experiment/Plac-failed-experiment)*

*[Stem Region Replacement](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/Pre_experiment/Stem_replacement)*

### H-stem System

*[H-stem Construction](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/H_Stem/Construction)*

*[H-stem Vector Exchange to pSB1A3](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/H_Stem/Replacement_To_1A3)*

*[H-stem Downstream Correction](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/H_Stem/Downstream_Correction)*

### Anti-Sense B0034

*[asB0034, asB0032 on 1A3 Construction](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/asB0034/asB0034%2C32-construction)*

*[asmRFP Construction](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/asB0034/asmRFP)*

*[asB0034, asB0032 on 1C3 Construction](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/asB0034/asB0034%2C32-1C3-construction)*

*[asE1010 on 1A3 Construction](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/asB0034/asE1010_1A3_Construction)*

*[E1010 Target gene vector exchange to pSB6A1](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/asB0034/asE1010_Target_gene_vector_Exchange_to_6A1)*

*[140910 asB0034 & asB0034 mRFP Assay](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/asB0034/140910_asB0034%26B0034_mRFP_Assay)*

*[140910 asB0034 & asB0034 GFP Assay](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/asB0034/140910_asB0034%26B0034_GFP_Assay)*

*[140910 asB0034 & asB0032 mRFP Assay](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/asB0034/140910_asB0034%26B0032_mRFP_Assay)*

*[Ptet-B0034,B0032,-mRFP-dT Ptet-B0032-mRFP-dT&asB0034Assay](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/asB0034/Ptet_B0034_32_asB0034_assay)*

### Length Variation

*[Anti-sense 90, 120 Construction](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/Length/as90_120)*

*[Developmental Examination](https://2014.igem.org/Team%3AHokkaidoU_Japan/Notebook/Length/Future_Work)*

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