iGEM 2017 — Team Bulgaria

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Bulgaria/Notebook

[Home](https://2017.igem.org/Team%3ABulgaria)
[Team](https://2017.igem.org/Team%3ABulgaria/team)

Project

[Description](https://2017.igem.org/Team%3ABulgaria/description)
[Design](https://2017.igem.org/Team%3ABulgaria/design)
[Experiments](https://2017.igem.org/Team%3ABulgaria/experiments)
[Results](https://2017.igem.org/Team%3ABulgaria/results)
[Contribution](https://2017.igem.org/Team%3ABulgaria/contribution)
[Improve](https://2017.igem.org/Team%3ABulgaria/improve)
[Second project](https://2017.igem.org/Team%3ABulgaria/second-project)
[Demonstrate](https://2017.igem.org/Team%3ABulgaria/Demonstrate)
[Lab book](https://2017.igem.org/Team%3ABulgaria/lab-book)
[Protocols](https://2017.igem.org/Team%3ABulgaria/protocols)

Human Practices

[Silver](https://2017.igem.org/Team%3ABulgaria/HP/Silver)
[Gold](https://2017.igem.org/Team%3ABulgaria/HP/Gold_Integrated)
[Survey](https://2017.igem.org/Team%3ABulgaria/survey)
[Public Engagements](https://2017.igem.org/Team%3ABulgaria/public-engagement)
[I, Engineer](https://2017.igem.org/Team%3ABulgaria/i-engineer)
[Fission festival](https://2017.igem.org/Team%3ABulgaria/fission-festival)
[International workshop Innovation corner](https://2017.igem.org/Team%3ABulgaria/international-workshop-innovation-corner)
[Sofia science festival](https://2017.igem.org/Team%3ABulgaria/sofia-science-festival)
[The European Researchers’ Night](https://2017.igem.org/Team%3ABulgaria/researchers-night)
[Bloomberg TV Bulgaria](https://2017.igem.org/Team%3ABulgaria/bloomberg-tv-bulgaria)
[BNT1 TV Bulgaria](https://2017.igem.org/Team%3ABulgaria/bnt1-tv-bulgaria)
[Agar Art - Bulgaria](https://2017.igem.org/Team%3ABulgaria/agar-art-bulgaria)
[BG Economics Magazine](https://2017.igem.org/Team%3ABulgaria/bg-science-magazine)
[Project Yana](https://2017.igem.org/Team%3ABulgaria/project-yana)
[Project Vyara](https://2017.igem.org/Team%3ABulgaria/project-vyara)

Achievements

[Parts](https://2017.igem.org/Team%3ABulgaria/parts)

[Collaborations](https://2017.igem.org/Team%3ABulgaria/Collaborations)
[Safety](https://2017.igem.org/Team%3ABulgaria/safety)
[Attributions](https://2017.igem.org/Team%3ABulgaria/Attributions)

# Lab book

### This is the official handwritten lab book of one of our team members: [here](https://static.igem.org/mediawiki/2017/d/da/Doc00033720171031175339.pdf)!

### You can also downloand a short English version with some gel electrophoresis pictures included: [here!](https://static.igem.org/mediawiki/2017/2/2a/Our-lab-book.pdf)

![](https://i.imgur.com/MVlPgPT.jpg)

## Our Partners

[![](https://static.igem.org/mediawiki/2016/2/29/Su-logo2.png)](https://www.uni-sofia.bg/)

[![](https://i.imgur.com/nX3mgLX.png)](https://www.venusconcept.com/)

[![](https://i.imgur.com/vNa55DS.png)](bulmed.bg/)

[![](https://i.imgur.com/B1HvUKI.png)](mu-sofia.bg)

[![](https://static.igem.org/mediawiki/2016/6/65/Tech-park-logo.png)](http://sofiatech.bg/)

[![](https://static.igem.org/mediawiki/2016/9/9e/Su-logo3.png)](https://students.uni-sofia.bg/)

[![](http://www.neb-online.fr/wp-content/uploads/neb_logo_409.png)](http://www.neb-online.fr/)

[![](https://i.imgur.com/RC4pMPf.png)](https://www.thermofisher.com/)

[![](https://i.imgur.com/NpIrZfq.png)](http://www.neb-online.fr/)

[![](https://static.igem.org/mediawiki/2016/9/97/Tu-logo.png)](http://tu-sofia.bg/)

[![](http://www.vipcatering.bg/images/logo_client222432/images/clients/npss.png?v4)](http://www.vipcatering.bg/)

[![](https://i.imgur.com/0x1tYBf.png)](https://www.idtdna.com/)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)



[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Bulgaria/Results

[Home](https://2017.igem.org/Team%3ABulgaria)
[Team](https://2017.igem.org/Team%3ABulgaria/team)

Project

[Description](https://2017.igem.org/Team%3ABulgaria/description)
[Design](https://2017.igem.org/Team%3ABulgaria/design)
[Experiments](https://2017.igem.org/Team%3ABulgaria/experiments)
[Results](https://2017.igem.org/Team%3ABulgaria/results)
[Contribution](https://2017.igem.org/Team%3ABulgaria/contribution)
[Improve](https://2017.igem.org/Team%3ABulgaria/improve)
[Second project](https://2017.igem.org/Team%3ABulgaria/second-project)
[Demonstrate](https://2017.igem.org/Team%3ABulgaria/Demonstrate)
[Lab book](https://2017.igem.org/Team%3ABulgaria/lab-book)
[Protocols](https://2017.igem.org/Team%3ABulgaria/protocols)

Human Practices

[Silver](https://2017.igem.org/Team%3ABulgaria/HP/Silver)
[Gold](https://2017.igem.org/Team%3ABulgaria/HP/Gold_Integrated)
[Survey](https://2017.igem.org/Team%3ABulgaria/survey)
[Public Engagements](https://2017.igem.org/Team%3ABulgaria/public-engagement)
[I, Engineer](https://2017.igem.org/Team%3ABulgaria/i-engineer)
[Fission festival](https://2017.igem.org/Team%3ABulgaria/fission-festival)
[International workshop Innovation corner](https://2017.igem.org/Team%3ABulgaria/international-workshop-innovation-corner)
[Sofia science festival](https://2017.igem.org/Team%3ABulgaria/sofia-science-festival)
[The European Researchers’ Night](https://2017.igem.org/Team%3ABulgaria/researchers-night)
[Bloomberg TV Bulgaria](https://2017.igem.org/Team%3ABulgaria/bloomberg-tv-bulgaria)
[BNT1 TV Bulgaria](https://2017.igem.org/Team%3ABulgaria/bnt1-tv-bulgaria)
[Agar Art - Bulgaria](https://2017.igem.org/Team%3ABulgaria/agar-art-bulgaria)
[BG Economics Magazine](https://2017.igem.org/Team%3ABulgaria/bg-science-magazine)
[Project Yana](https://2017.igem.org/Team%3ABulgaria/project-yana)
[Project Vyara](https://2017.igem.org/Team%3ABulgaria/project-vyara)

Achievements

[Parts](https://2017.igem.org/Team%3ABulgaria/parts)

[Collaborations](https://2017.igem.org/Team%3ABulgaria/Collaborations)
[Safety](https://2017.igem.org/Team%3ABulgaria/safety)
[Attributions](https://2017.igem.org/Team%3ABulgaria/Attributions)

# Results

### **gRNA vector**

### **Mutator Strain**

We tested our mutator strain via plating cultures with gRNA arrays that suppress genes with an important role in different reparation and/or replication fidelity systems. As a readout we used the number of rifampicin resistant colonies. Unfortunately our results so far are inconclusive due to the small experiment number. We hope that this will change until our presentation on the Giant Jamboree.

## Our Partners

[![](https://static.igem.org/mediawiki/2016/2/29/Su-logo2.png)](https://www.uni-sofia.bg/)

[![](https://i.imgur.com/nX3mgLX.png)](https://www.venusconcept.com/)

[![](https://i.imgur.com/vNa55DS.png)](bulmed.bg/)

[![](https://i.imgur.com/B1HvUKI.png)](mu-sofia.bg)

[![](https://static.igem.org/mediawiki/2016/6/65/Tech-park-logo.png)](http://sofiatech.bg/)

[![](https://static.igem.org/mediawiki/2016/9/9e/Su-logo3.png)](https://students.uni-sofia.bg/)

[![](http://www.neb-online.fr/wp-content/uploads/neb_logo_409.png)](http://www.neb-online.fr/)

[![](https://i.imgur.com/RC4pMPf.png)](https://www.thermofisher.com/)

[![](https://i.imgur.com/NpIrZfq.png)](http://www.neb-online.fr/)

[![](https://static.igem.org/mediawiki/2016/9/97/Tu-logo.png)](http://tu-sofia.bg/)

[![](http://www.vipcatering.bg/images/logo_client222432/images/clients/npss.png?v4)](http://www.vipcatering.bg/)

[![](https://i.imgur.com/0x1tYBf.png)](https://www.idtdna.com/)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)