iGEM 2020 — Team NWU-CHINA-B

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# Team:NWU-CHINA-B/Notebook

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> 新型冠状病毒疫情在全球范围内的肆虐，给我们带来了沉重的灾难，但是在防治过程中的表现证明了我们有能力扛过这次难关。在疫情防治过程中，中草药的作用再次突出，为挽救人们的生命做出了贡献。而那些中草药的都有一个共同的成分：山奈酚。
> 山奈酚是一种常见的物质，广泛存在于生活中的果蔬中，拥有抗氧化、抗菌、抗炎等多种功效并且安全无毒。中国古代就运用合金欢，银杏叶，桂皮等含有山奈酚的中药来消炎和治病。而在现代社会山奈酚也经常被人们用于防癌抗癌，抗感染等。
> 研究发现，山奈酚具有很好的抗癌、防癌的生物学活性，能够显著抑制白血病的发生，以及抑制肺癌 、结肠癌、肝癌、乳腺癌、膀胱癌等多种恶性肿瘤细胞的生长，并诱导癌细胞凋亡 ，其本身也可以与其他肿瘤治疗方法结合使用，加强肿瘤治疗效果。
> 山奈酚在防治糖尿病也表现出优秀的能力。它可以通过降低血糖、改善胰岛素敏感度来维持正常血脂水平。
> 氧化应激反应在一些神经退行性疾病患者中经常出现，包括帕金森病和阿尔茨海默病。山奈酚能过抑制氧化应激反应，对由氧化应激引起的神经损伤起到一定的保护作用。
> 山奈酚还具有十分明显的抗炎作用，其作用机制主要包括调节促炎酶的活性和炎症相关基因表达，抑制转录因子、黏附分子以及基质金属蛋白酶等。
> 除上述生物功能外，人们发现，山奈酚还具有抗菌作用，对金黄色葡萄球菌、绿脓杆菌、伤寒杆菌等多种人体病原菌均有很好的抑制作用，还在止咳、治疗白内障、抑制生育、抗癫痫、痉、抗溃疡、利胆利尿等方面表现出了一定的治疗效果。此外，山奈酚还是一种天然的免疫抑制剂，可以减少由 T 淋巴细胞过度活化和增殖所导致的自身免疫病和器官移植排斥反应的发生。
> 山奈酚应用广泛，效果显著，受到各界关注，但山奈酚由于其平面型的分子结构，堆砌较紧密，分子间的引力较大，不易被溶剂或溶质分散，水溶性较差，机体难以吸收、代谢，这些特点极大的限制了其生物利用度、体内的给药途径和药效的发挥。药物的溶出速度对于药物的吸收很关键，固体制剂必须在胃肠液中先溶解，然后才能被粘膜吸收，如果药物的在胃肠液中溶解的速度太慢，就会影响药物的吸收。并且山奈酚的药理活性常常呈浓度依赖关系，这就要求我们在不改变药效母核、力求保持其药理活性结构的条件下，引进亲水基团，增强其水溶性，改善其药途径，增强药物吸收能力。
> 因此我们提出了一一个想法:将- -个糖基合成到山奈酚上，使其拥有更好的水溶性。这样就能够在不改变山奈酚药理活性结构条件下，使其更好的发挥药效作用。

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:NWU-CHINA-B/Engineering

Description

Why do we choose kaempferol

Since December 2019, the novel coronavirus epidemic has spread worldwide. The WHO (WHO) has classified the new coronavirus epidemic as a public health emergencies of international concern (PHEIC). In the face of unexpected problems, we have found a common ingredient in many therapeutic drugs, kaempferol, which has played an important role in anti-virus and anti-inflammatory during the epidemic.

Kaempferol is insoluble in water, which limits its application in anti-virus and anti-inflammation, while glycosylation can enhance the water solubility of phenols, which is beneficial to the absorption of drugs, and may even enhance the properties of some aspects. we want to verify whether the water solubility of kaempferol will be enhanced by adding 1 or 2 glycosyl groups, and whether its anti-inflammation still exists, or even stronger.

The plasmid of glycosylated kaempferol

Two plasmids carrying two UDP glycosyltransferases were designed. Kaempferol was added 1 or 2 glycosyl groups by transforming E. coli DE3, to obtain Astragalin and Kaempferol 3-O-glucoside-7-O-rhamnoside. The recombinant E. coli was cultured overnight, IPTG was added to induce the expression of the recombinant protein, and then the protein was purified to construct an in vitro enzymatic reaction system to produce the product under suitable conditions.
Finally, the glycosylation products of kaempferol were detected and purified by HPLC combined with mass spectrometry.

After obtaining the product, we test its water solubility and anti-inflammation, and compare it with the kaempferol control group to verify whether glycosylation can improve the water solubility of kaempferol and whether it can maintain or even enhance its anti-inflammation.

Expansion and extension

On the one hand, we will work with the NWU-CHINA-A team to put the product on the band-aid. Our glycosylated kaempferol combines with their antimicrobial peptides to produce antibacterial and anti-inflammatory band-aids that can effectively prevent wound infection.

On the other hand, in the future, we also hope to work with pharmaceutical companies to add glycosylated kaempferol to drugs to treat inflammation caused by viruses and bacteria.

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:NWU-CHINA-B/Experiments

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> 新型冠状病毒疫情在全球范围内的肆虐，给我们带来了沉重的灾难，但是在防治过程中的表现证明了我们有能力扛过这次难关。在疫情防治过程中，中草药的作用再次突出，为挽救人们的生命做出了贡献。而那些中草药的都有一个共同的成分：山奈酚。
> 山奈酚是一种常见的物质，广泛存在于生活中的果蔬中，拥有抗氧化、抗菌、抗炎等多种功效并且安全无毒。中国古代就运用合金欢，银杏叶，桂皮等含有山奈酚的中药来消炎和治病。而在现代社会山奈酚也经常被人们用于防癌抗癌，抗感染等。
> 研究发现，山奈酚具有很好的抗癌、防癌的生物学活性，能够显著抑制白血病的发生，以及抑制肺癌 、结肠癌、肝癌、乳腺癌、膀胱癌等多种恶性肿瘤细胞的生长，并诱导癌细胞凋亡 ，其本身也可以与其他肿瘤治疗方法结合使用，加强肿瘤治疗效果。
> 山奈酚在防治糖尿病也表现出优秀的能力。它可以通过降低血糖、改善胰岛素敏感度来维持正常血脂水平。
> 氧化应激反应在一些神经退行性疾病患者中经常出现，包括帕金森病和阿尔茨海默病。山奈酚能过抑制氧化应激反应，对由氧化应激引起的神经损伤起到一定的保护作用。
> 山奈酚还具有十分明显的抗炎作用，其作用机制主要包括调节促炎酶的活性和炎症相关基因表达，抑制转录因子、黏附分子以及基质金属蛋白酶等。
> 除上述生物功能外，人们发现，山奈酚还具有抗菌作用，对金黄色葡萄球菌、绿脓杆菌、伤寒杆菌等多种人体病原菌均有很好的抑制作用，还在止咳、治疗白内障、抑制生育、抗癫痫、痉、抗溃疡、利胆利尿等方面表现出了一定的治疗效果。此外，山奈酚还是一种天然的免疫抑制剂，可以减少由 T 淋巴细胞过度活化和增殖所导致的自身免疫病和器官移植排斥反应的发生。
> 山奈酚应用广泛，效果显著，受到各界关注，但山奈酚由于其平面型的分子结构，堆砌较紧密，分子间的引力较大，不易被溶剂或溶质分散，水溶性较差，机体难以吸收、代谢，这些特点极大的限制了其生物利用度、体内的给药途径和药效的发挥。药物的溶出速度对于药物的吸收很关键，固体制剂必须在胃肠液中先溶解，然后才能被粘膜吸收，如果药物的在胃肠液中溶解的速度太慢，就会影响药物的吸收。并且山奈酚的药理活性常常呈浓度依赖关系，这就要求我们在不改变药效母核、力求保持其药理活性结构的条件下，引进亲水基团，增强其水溶性，改善其药途径，增强药物吸收能力。
> 因此我们提出了一一个想法:将- -个糖基合成到山奈酚上，使其拥有更好的水溶性。这样就能够在不改变山奈酚药理活性结构条件下，使其更好的发挥药效作用。

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:NWU-CHINA-B/Results

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无标题文档

|  |  |  |
| --- | --- | --- |
| HOME | TEAM | PROJECT |

|  |  |  |  |
| --- | --- | --- | --- |
|  |  |  |  |

> 新型冠状病毒疫情在全球范围内的肆虐，给我们带来了沉重的灾难，但是在防治过程中的表现证明了我们有能力扛过这次难关。在疫情防治过程中，中草药的作用再次突出，为挽救人们的生命做出了贡献。而那些中草药的都有一个共同的成分：山奈酚。
> 山奈酚是一种常见的物质，广泛存在于生活中的果蔬中，拥有抗氧化、抗菌、抗炎等多种功效并且安全无毒。中国古代就运用合金欢，银杏叶，桂皮等含有山奈酚的中药来消炎和治病。而在现代社会山奈酚也经常被人们用于防癌抗癌，抗感染等。
> 研究发现，山奈酚具有很好的抗癌、防癌的生物学活性，能够显著抑制白血病的发生，以及抑制肺癌 、结肠癌、肝癌、乳腺癌、膀胱癌等多种恶性肿瘤细胞的生长，并诱导癌细胞凋亡 ，其本身也可以与其他肿瘤治疗方法结合使用，加强肿瘤治疗效果。
> 山奈酚在防治糖尿病也表现出优秀的能力。它可以通过降低血糖、改善胰岛素敏感度来维持正常血脂水平。
> 氧化应激反应在一些神经退行性疾病患者中经常出现，包括帕金森病和阿尔茨海默病。山奈酚能过抑制氧化应激反应，对由氧化应激引起的神经损伤起到一定的保护作用。
> 山奈酚还具有十分明显的抗炎作用，其作用机制主要包括调节促炎酶的活性和炎症相关基因表达，抑制转录因子、黏附分子以及基质金属蛋白酶等。
> 除上述生物功能外，人们发现，山奈酚还具有抗菌作用，对金黄色葡萄球菌、绿脓杆菌、伤寒杆菌等多种人体病原菌均有很好的抑制作用，还在止咳、治疗白内障、抑制生育、抗癫痫、痉、抗溃疡、利胆利尿等方面表现出了一定的治疗效果。此外，山奈酚还是一种天然的免疫抑制剂，可以减少由 T 淋巴细胞过度活化和增殖所导致的自身免疫病和器官移植排斥反应的发生。
> 山奈酚应用广泛，效果显著，受到各界关注，但山奈酚由于其平面型的分子结构，堆砌较紧密，分子间的引力较大，不易被溶剂或溶质分散，水溶性较差，机体难以吸收、代谢，这些特点极大的限制了其生物利用度、体内的给药途径和药效的发挥。药物的溶出速度对于药物的吸收很关键，固体制剂必须在胃肠液中先溶解，然后才能被粘膜吸收，如果药物的在胃肠液中溶解的速度太慢，就会影响药物的吸收。并且山奈酚的药理活性常常呈浓度依赖关系，这就要求我们在不改变药效母核、力求保持其药理活性结构的条件下，引进亲水基团，增强其水溶性，改善其药途径，增强药物吸收能力。
> 因此我们提出了一一个想法:将- -个糖基合成到山奈酚上，使其拥有更好的水溶性。这样就能够在不改变山奈酚药理活性结构条件下，使其更好的发挥药效作用。

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)