iGEM 2017 — Team Gaston_Day_School

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# Team:Gaston Day School/Notebook

* [Project](https://2017.igem.org/Team%3AGaston_Day_School/Summary)
  + [Summary](https://2017.igem.org/Team%3AGaston_Day_School/Demonstrate)
  + [Experiment](https://2017.igem.org/Team%3AGaston_Day_School/Experiment)
  + [Notebook](https://2017.igem.org/Team%3AGaston_Day_School/Notebook)
  + [Interlab](https://2017.igem.org/Team%3AGaston_Day_School/InterLab)
* [Part](https://2017.igem.org/Team%3AGaston_Day_School/Part)
  + [Part](https://2017.igem.org/Team%3AGaston_Day_School/Part)
  + [Part Collection](https://2017.igem.org/Team%3AGaston_Day_School/Part_Collection)
* [Safety](https://2017.igem.org/Team%3AGaston_Day_School/Safety)
* [Human Practices](https://2017.igem.org/Team%3AGaston_Day_School/Human_Practices)
  + [Human Practices](https://2017.igem.org/Team%3AGaston_Day_School/HP/Silver)
  + [Integrated Practices](https://2017.igem.org/Team%3AGaston_Day_School/HP/Gold_Integrated)
  + [Public Engagements](https://2017.igem.org/Team%3AGaston_Day_School/Engagement)
  + [Collaborations](https://2017.igem.org/Team%3AGaston_Day_School/Collaborations)
* [Medals](https://2017.igem.org/Team%3AGaston_Day_School/Medals)
* [Team](https://2017.igem.org/Team%3AGaston_Day_School/Team_Introduction)
  + [Team Introduction](https://2017.igem.org/Team%3AGaston_Day_School/Team_Introduction)
  + [Attributions](https://2017.igem.org/Team%3AGaston_Day_School/Attributions)

# Notebook

On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.

#### [09/01/2017 PCR Primer](#P1-collapse)

Master Mix:

|  |  |  |
| --- | --- | --- |
| 14.3 | H2O | 114.4 |
| 2.5 | Buffer | 20 |
| 1 | MgCl2 | 8 |
| 1 | dNTP | 8 |

tube:

|  |  |
| --- | --- |
| 2ul | H2O |
| -5ul | F |
| -5ul | R |

Label:

|  |  |
| --- | --- |
| 0 | Control |
| 1 | AceF |
| 2 | EutG BB |
| 3 | YiaY BB |
| 4 | AdhP BB |
| 5 | Lpd |
| 6 | AceE |

#### [09/06/2017 Genomic PCR](#P2-collapse)

Master Mix

|  |  |  |
| --- | --- | --- |
| gDNA | 3 | 24 |
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| dH2O | 13.3 | 106.4 |
| Taq | 0.2 | 1.6 |

|  | dH2O | For | Rev |  |
| --- | --- | --- | --- | --- |
| 1 | 4 | 0.5 | 0.5 | AceF |
| 2 | 4 | 0.5 | 0.5 | EutG |
| 3 | 4 | 0.5 | 0.5 | YiaY |
| 4 | 4 | 0.5 | 0.5 | AdnP |
| 5 | 4 | 0.5 | 0.5 | Lpd |
| 6 | 4 | 0.5 | 0.5 | AceE |
| 7 | 5 | - | - | - |

#### [09/11/2017 Genomic PCR](#P3-collapse)

Master Mix

|  |  |  |
| --- | --- | --- |
| gDNA | 3 | 24 |
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| dH2O | 12.3 | 98.4 |
| Taq | 0.2 | 1.6 |
| MgCl2 | 1 | 8 |

|  | dH2O | For | Rev |  |
| --- | --- | --- | --- | --- |
| 0 | 5 | - | - |  |
| 1 | 4 | 0.5 | 0.5 | ACE F |
| 2 | 4 | 0.5 | 0.5 | EutG |
| 3 | 4 | 0.5 | 0.5 | YiaY |
| 4 | 4 | 0.5 | 0.5 | AdhP |
| 5 | 4 | 0.5 | 0.5 | Lpd |
| 6 | 4 | 0.5 | 0.5 | AceE |

#### [09/18/2017 PCR check of J23102+GlmY](#P4-collapse)

1 | - | DNA(0) | dH2O(5) || 2 | J23102 | 3 | 2 |
| 3 | GlmY | 3 | 2 |
| 4 | A(1) | 3 | 2 |
| 5/td> A(2) | 3 | 2 | |
| 6 | B(2) | 3 | 2 |
| 7 | C(2) | 3 | 2 |

Max:

|  |  |  |
| --- | --- | --- |
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| VR | 0.5 | 4 |
| VF2 | 0.5 | 4 |
| Taq | 0.2 | 1.6 |
| dH2O | 15.3 | 122.4 |

#### [09/25/2017 PCR check for J23102+GlmY](#P3-collapse)

Master Mix

|  |  |  |
| --- | --- | --- |
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| VR | 0.5 | 4 |
| VF2 | 0.5 | 4 |
| taq | 0.2 | 1.6 |
| dH2O | 15.3 | 122.4 |

|  | DNA | H2O || 1 | Control | 0u | 5u |
| 2 | J231013 | 3 | 2 |
| 3 | GlmY-3A | 3 | 2 |
| 4 | 2C | 3 | 2 |
| 5 | 1C | 3 | 2 |
| 6 | 1B | 3 | 2 |
| 7 | 1A | 3 | 2 |

#### [10/10/2017 PCR check for J23102+GlmY](#P5-collapse)

Master Mix

|  |  |  |
| --- | --- | --- |
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| VR | 0.5 | 4 |
| VF2 | 0.5 | 4 |
| Taq | 0.2 | 1.6 |
| dH2O | 15.3 | 122.4 |

|  | DNA | H2O || 1 | Control | 0u | 5u |
| 2 | J231013 | 3 | 2 |
| 3 | GlmY-3A | 3 | 2 |
| 4 | 2C | 3 | 2 |
| 5 | 1C | 3 | 2 |
| 6 | 1B | 3 | 2 |
| 7 | 1A | 3 | 2 |

![](https://static.igem.org/mediawiki/2017/3/39/IMG_0207.jpeg)

#### [10/20/2017 PCR J23102+GlmY](#P6-collapse)

Master Mix

|  |  |  |
| --- | --- | --- |
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| VR | 0.5 | 4 |
| VF2 | 0.5 | 4 |
| Taq | 0.2 | 1.6 |
| dH2O | 15.3 | 122.4 |

|  | DNA | H2O || 1 | Control | 0u | 5u |
| 2 | J23102B pp | 3 | 2 |
| 3 | J23102 P NNP | 3 | 2 |
| 4 | GlmY-3A | 3 | 2 |
| 5 | 102B+GlmY 1B | 3 | 2 |
| 6 | 102B+GlmY 1C | 3 | 2 |
| 7 | 102B\_GlmY 1D | 3 | 2 |

![](https://static.igem.org/mediawiki/2017/5/54/IMG_0674.jpeg)

#### [10/23/2017 Genomic PCR](#P7-collapse)

Master Mix

|  |  |  |
| --- | --- | --- |
| H2O | 13.3 | 106.4 |
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| GDNA | 3 | 24 |
| Taq | 0.2 | 1.6 |

Gel:

|  | dH2O | For | Rev |  |
| --- | --- | --- | --- | --- |
| 1 | 5 | - | - |  |
| 2 | 4 | 0.5 | 0.5 | AceF |
| 3 | 4 | 0.5 | 0.5 | EutG |
| 4 | 4 | 0.5 | 0.5 | YiaY |
| 5 | 4 | 0.5 | 0.5 | AdhP |
| 6 | 4 | 0.5 | 0.5 | Lpd |
| 7 | 4 | 0.5 | 0.5 | AceE |

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Email: iGEM@gastonday.org

Gaston Day School

2001 Gaston Day School Rd

Gastonia 28056

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# Team:Gaston Day School/Experiments

* [Project](https://2017.igem.org/Team%3AGaston_Day_School/Summary)
  + [Summary](https://2017.igem.org/Team%3AGaston_Day_School/Demonstrate)
  + [Experiment](https://2017.igem.org/Team%3AGaston_Day_School/Experiment)
  + [Notebook](https://2017.igem.org/Team%3AGaston_Day_School/Notebook)
  + [Interlab](https://2017.igem.org/Team%3AGaston_Day_School/InterLab)
* [Part](https://2017.igem.org/Team%3AGaston_Day_School/Part)
  + [Part](https://2017.igem.org/Team%3AGaston_Day_School/Part)
  + [Part Collection](https://2017.igem.org/Team%3AGaston_Day_School/Part_Collection)
* [Safety](https://2017.igem.org/Team%3AGaston_Day_School/Safety)
* [Human Practices](https://2017.igem.org/Team%3AGaston_Day_School/Human_Practices)
  + [Human Practices](https://2017.igem.org/Team%3AGaston_Day_School/HP/Silver)
  + [Integrated Practices](https://2017.igem.org/Team%3AGaston_Day_School/HP/Gold_Integrated)
  + [Public Engagements](https://2017.igem.org/Team%3AGaston_Day_School/Engagement)
  + [Collaborations](https://2017.igem.org/Team%3AGaston_Day_School/Collaborations)
* [Medals](https://2017.igem.org/Team%3AGaston_Day_School/Medals)
* [Team](https://2017.igem.org/Team%3AGaston_Day_School/Team_Introduction)
  + [Team Introduction](https://2017.igem.org/Team%3AGaston_Day_School/Team_Introduction)
  + [Attributions](https://2017.igem.org/Team%3AGaston_Day_School/Attributions)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

##### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

##### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Gaston Day School/Results

* [Project](https://2017.igem.org/Team%3AGaston_Day_School/Summary)
  + [Summary](https://2017.igem.org/Team%3AGaston_Day_School/Demonstrate)
  + [Experiment](https://2017.igem.org/Team%3AGaston_Day_School/Experiment)
  + [Notebook](https://2017.igem.org/Team%3AGaston_Day_School/Notebook)
  + [Interlab](https://2017.igem.org/Team%3AGaston_Day_School/InterLab)
* [Part](https://2017.igem.org/Team%3AGaston_Day_School/Part)
  + [Part](https://2017.igem.org/Team%3AGaston_Day_School/Part)
  + [Part Collection](https://2017.igem.org/Team%3AGaston_Day_School/Part_Collection)
* [Safety](https://2017.igem.org/Team%3AGaston_Day_School/Safety)
* [Human Practices](https://2017.igem.org/Team%3AGaston_Day_School/Human_Practices)
  + [Human Practices](https://2017.igem.org/Team%3AGaston_Day_School/HP/Silver)
  + [Integrated Practices](https://2017.igem.org/Team%3AGaston_Day_School/HP/Gold_Integrated)
  + [Public Engagements](https://2017.igem.org/Team%3AGaston_Day_School/Engagement)
  + [Collaborations](https://2017.igem.org/Team%3AGaston_Day_School/Collaborations)
* [Medals](https://2017.igem.org/Team%3AGaston_Day_School/Medals)
* [Team](https://2017.igem.org/Team%3AGaston_Day_School/Team_Introduction)
  + [Team Introduction](https://2017.igem.org/Team%3AGaston_Day_School/Team_Introduction)
  + [Attributions](https://2017.igem.org/Team%3AGaston_Day_School/Attributions)

# Results

Here you can describe the results of your project and your future plans.

##### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

##### You should also describe what your results mean:

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

##### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

##### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)