iGEM 2011 — Team Minnesota

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# Team:Minnesota/Notebook

### From 2011.igem.org

[![Mnlogo.jpg](/wiki/images/8/89/Mnlogo.jpg)](/File%3AMnlogo.jpg)

| [Home](/Team%3AMinnesota "Team:Minnesota") | [Team](/Team%3AMinnesota/Team "Team:Minnesota/Team") | [Project](/Team%3AMinnesota/Project "Team:Minnesota/Project") | [Software](/Team%3AMinnesota/Software "Team:Minnesota/Software") | [Protocols](/Team%3AMinnesota/Protocols "Team:Minnesota/Protocols") | **Notebook** | [Attributions](/Team%3AMinnesota/Attributions "Team:Minnesota/Attributions") | [Safety](/Team%3AMinnesota/Safety "Team:Minnesota/Safety") |
| --- | --- | --- | --- | --- | --- | --- | --- |

# iGEM 2011 Laboratory Notebook

## Project: Regulatory

### Date: Tuesday, 29 March 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 29 Mar 2011 12:00:00 GMT | Nathan Davis | [Transformation of comp. cells with registry parts](/Team%3AMinnesota/davis950-03292011120000-1 "Team:Minnesota/davis950-03292011120000-1") |

### Date: Thursday, 07 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 07 Apr 2011 15:14:00 GMT | Nathan Davis | [EnvZ mutant comp. cells made](/Team%3AMinnesota/davis950-04072011151400-3 "Team:Minnesota/davis950-04072011151400-3") |

### Date: Monday, 18 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 18 Apr 2011 12:00:00 GMT | Nathan Davis | [Registry parts and results of X-gal assay of I15008](/Team%3AMinnesota/davis950-04182011120000-4 "Team:Minnesota/davis950-04182011120000-4") |

### Date: Thursday, 21 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 21 Apr 2011 12:00:00 GMT | Nathan Davis | [Transformation of pCph8 from Voigt lab](/Team%3AMinnesota/davis950-04212011120000-5 "Team:Minnesota/davis950-04212011120000-5") |

### Date: Thursday, 26 May 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 26 May 2011 12:00:00 GMT | Nathan Davis | [PCR of Syneccocystis sp 6803 genomic DNA and pCph8 miniprep](/Team%3AMinnesota/davis950-05262011120000-7 "Team:Minnesota/davis950-05262011120000-7") |

### Date: Monday, 18 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 18 Jul 2011 14:59:48 GMT | Nathan Davis | [Transformation of comp. cell with registry parts](/Team%3AMinnesota/davis950-07182011145948-2 "Team:Minnesota/davis950-07182011145948-2") |
| Mon, 18 Jul 2011 15:28:28 GMT | Nathan Davis | [Transformation with pUC-BB, pUCbb-pBad, and pUCbb-pTet](/Team%3AMinnesota/davis950-07182011152828-8 "Team:Minnesota/davis950-07182011152828-8") |

### Date: Wednesday, 20 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 20 Jul 2011 16:14:10 GMT | Nathan Davis | [Miniprep of pUCbb-pBad and pUCbb-pTet](/Team%3AMinnesota/davis950-07202011161410-9 "Team:Minnesota/davis950-07202011161410-9") |

### Date: Friday, 22 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 22 Jul 2011 19:58:49 GMT | Sung Bong Lee | [run gel with pcr product of ho1 and cph 8](/Team%3AMinnesota/leex3772-07222011195849-10 "Team:Minnesota/leex3772-07222011195849-10") |

### Date: Monday, 25 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 25 Jul 2011 14:23:53 GMT | Sung Bong Lee | [Gel extraction of PCR product of ho1 and Cph8](/Team%3AMinnesota/leex3772-07252011142353-11 "Team:Minnesota/leex3772-07252011142353-11") |

### Date: Thursday, 28 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 28 Jul 2011 20:25:02 GMT | Sung Bong Lee | [Run gel with NEW PCR product of ho1 and cph8](/Team%3AMinnesota/leex3772-07282011202502-12 "Team:Minnesota/leex3772-07282011202502-12") |

### Date: Wednesday, 03 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 03 Aug 2011 16:41:47 GMT | Nathan Davis | [Gel extraction of Cph8 and ho1](/Team%3AMinnesota/davis950-08032011164147-13 "Team:Minnesota/davis950-08032011164147-13") |
| Wed, 03 Aug 2011 16:44:51 GMT | Nathan Davis | [Digest of ho1 and Cph8](/Team%3AMinnesota/davis950-08032011164451-14 "Team:Minnesota/davis950-08032011164451-14") |

### Date: Wednesday, 10 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 10 Aug 2011 14:59:42 GMT | Nathan Davis | [Transformation of pucBB-pBad-ho1, pucBB-pBad-pcyA, and pucBB-pTet-Cph8](/Team%3AMinnesota/davis950-08102011145942-15 "Team:Minnesota/davis950-08102011145942-15") |

### Date: Saturday, 13 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sat, 13 Aug 2011 12:35:20 GMT | Nathan Davis | [Miniprep of pucBB-pTet-Cph8, pucBB-pTet-pcyA, and pucBB-pTet-ho1](/Team%3AMinnesota/davis950-08132011123520-16 "Team:Minnesota/davis950-08132011123520-16") |

### Date: Tuesday, 16 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 16 Aug 2011 13:55:44 GMT | Nathan Davis | [Digest of pcyA and ho1](/Team%3AMinnesota/davis950-08162011135544-17 "Team:Minnesota/davis950-08162011135544-17") |

### Date: Tuesday, 23 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 23 Aug 2011 15:50:51 GMT | Nathan Davis | [Digest of pUCBB-pBad-pcyA pUCBB-pBad-ho1](/Team%3AMinnesota/davis950-08232011155051-72 "Team:Minnesota/davis950-08232011155051-72") |

### Date: Tuesday, 30 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 30 Aug 2011 13:22:13 GMT | Nathan Davis | [Gel extraction and ligation of pcyA and ho1](/Team%3AMinnesota/davis950-08302011132213-114 "Team:Minnesota/davis950-08302011132213-114") |
| Tue, 30 Aug 2011 13:29:13 GMT | Nathan Davis | [PCR of ompR and silicatein](/Team%3AMinnesota/davis950-08302011132913-115 "Team:Minnesota/davis950-08302011132913-115") |

### Date: Thursday, 01 September 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 01 Sep 2011 14:14:48 GMT | Nathan Davis | [Gel extraction of ompR and silicatein](/Team%3AMinnesota/davis950-09012011141448-116 "Team:Minnesota/davis950-09012011141448-116") |
| Thu, 01 Sep 2011 14:18:25 GMT | Nathan Davis | [Transformation of pcyA/ho1 ligation product](/Team%3AMinnesota/davis950-09012011141825-117 "Team:Minnesota/davis950-09012011141825-117") |

### Date: Friday, 09 September 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 09 Sep 2011 11:45:17 GMT | Nathan Davis | [Miniprep of pUC-pcyA-ho1](/Team%3AMinnesota/davis950-09092011114517-118 "Team:Minnesota/davis950-09092011114517-118") |
| Fri, 09 Sep 2011 12:13:01 GMT | Nathan Davis | [Digest of pUC-pcyA-ho1 and pUC-pTet-Cph8](/Team%3AMinnesota/davis950-09092011121301-119 "Team:Minnesota/davis950-09092011121301-119") |

### Date: Saturday, 10 September 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sat, 10 Sep 2011 13:02:58 GMT | Nathan Davis | [Transformation of pUC-pcyA-ho1-Cph8](/Team%3AMinnesota/davis950-09102011130258-120 "Team:Minnesota/davis950-09102011130258-120") |
| Sat, 10 Sep 2011 13:05:03 GMT | Nathan Davis | [Miniprep of pUCBB-lacZ and pBBR](/Team%3AMinnesota/davis950-09102011130503-121 "Team:Minnesota/davis950-09102011130503-121") |
| Sat, 10 Sep 2011 13:06:54 GMT | Nathan Davis | [Digest of pUCBB-lacZ, pBBR, and ompR](/Team%3AMinnesota/davis950-09102011130654-122 "Team:Minnesota/davis950-09102011130654-122") |

## Project: Surface Display

### Date: Thursday, 07 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 07 Apr 2011 11:20:00 GMT | Sukdong Lee | [Primer Design for OmpA](/Team%3AMinnesota/leex4462-04072011112000-18 "Team:Minnesota/leex4462-04072011112000-18") |

### Date: Sunday, 10 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sun, 10 Apr 2011 00:00:00 GMT | Sukdong Lee | [OmpA BL21 Genomic Liquid Cultures](/Team%3AMinnesota/leex4462-04102011000000-19 "Team:Minnesota/leex4462-04102011000000-19") |

### Date: Monday, 11 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 11 Apr 2011 00:00:00 GMT | Sukdong Lee | [OmpA BL21 Genomic Extraction](/Team%3AMinnesota/leex4462-04112011000000-20 "Team:Minnesota/leex4462-04112011000000-20") |

### Date: Thursday, 14 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 14 Apr 2011 00:00:00 GMT | Sukdong Lee | [PCR Amplification of OmpA](/Team%3AMinnesota/leex4462-04142011000000-21 "Team:Minnesota/leex4462-04142011000000-21") |
| Thu, 14 Apr 2011 00:00:00 GMT | Sukdong Lee | [pucBB Liquid Cultures](/Team%3AMinnesota/leex4462-04142011000000-22 "Team:Minnesota/leex4462-04142011000000-22") |
| Thu, 14 Apr 2011 00:00:00 GMT | Sukdong Lee | [Gel Electrophoresis of OmpA PCR Product](/Team%3AMinnesota/leex4462-04142011000000-23 "Team:Minnesota/leex4462-04142011000000-23") |
| Thu, 14 Apr 2011 00:00:00 GMT | Sukdong Lee | [Gel Purification of OmpA PCR Products](/Team%3AMinnesota/leex4462-04142011000000-24 "Team:Minnesota/leex4462-04142011000000-24") |

### Date: Friday, 15 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 15 Apr 2011 00:00:00 GMT | Sukdong Lee | [Miniprep of pucBB+GFP](/Team%3AMinnesota/leex4462-04152011000000-25 "Team:Minnesota/leex4462-04152011000000-25") |
| Fri, 15 Apr 2011 00:00:00 GMT | Sukdong Lee | [Restriction Digest of pucBB+GFP and Gel extraction of OmpA](/Team%3AMinnesota/leex4462-04152011000000-26 "Team:Minnesota/leex4462-04152011000000-26") |

### Date: Saturday, 16 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sat, 16 Apr 2011 00:01:00 GMT | Sukdong Lee | [Gel Electrophoresis of Restriction Digest of OmpA and pucBB+GFP](/Team%3AMinnesota/leex4462-04162011000100-27 "Team:Minnesota/leex4462-04162011000100-27") |
| Sat, 16 Apr 2011 00:02:00 GMT | Sukdong Lee | [Restriction Digest of pucBB and ompA inserts](/Team%3AMinnesota/leex4462-04162011000200-28 "Team:Minnesota/leex4462-04162011000200-28") |
| Sat, 16 Apr 2011 00:03:00 GMT | Sukdong Lee | [Gel Electrophoresis of OmpA](/Team%3AMinnesota/leex4462-04162011000300-29 "Team:Minnesota/leex4462-04162011000300-29") |

### Date: Tuesday, 19 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 19 Apr 2011 00:00:00 GMT | Sukdong Lee | [PCR Amplification of Genomic BL21](/Team%3AMinnesota/leex4462-04192011000000-30 "Team:Minnesota/leex4462-04192011000000-30") |
| Tue, 19 Apr 2011 00:01:00 GMT | Sukdong Lee | [Gel Extraction of OmpA](/Team%3AMinnesota/leex4462-04192011000100-31 "Team:Minnesota/leex4462-04192011000100-31") |

### Date: Wednesday, 20 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 20 Apr 2011 00:00:00 GMT | Sukdong Lee | [pBAD plasmid liquid cultures](/Team%3AMinnesota/leex4462-04202011000000-32 "Team:Minnesota/leex4462-04202011000000-32") |

### Date: Thursday, 21 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 21 Apr 2011 00:00:00 GMT | Sukdong Lee | [Miniprep, Restriction Digest of OmpA and pucBB, and Gel extraction](/Team%3AMinnesota/leex4462-04212011000000-33 "Team:Minnesota/leex4462-04212011000000-33") |
| Thu, 21 Apr 2011 12:00:00 GMT | Nathan Davis | [Gel extraction of Silicatein alpha DNA](/Team%3AMinnesota/davis950-04212011120000-6 "Team:Minnesota/davis950-04212011120000-6") |

### Date: Friday, 22 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 22 Apr 2011 00:00:00 GMT | Sukdong Lee | [Miniprep, Digest, and Extract](/Team%3AMinnesota/leex4462-04222011000000-40 "Team:Minnesota/leex4462-04222011000000-40") |

### Date: Saturday, 23 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sat, 23 Apr 2011 00:00:00 GMT | Sukdong Lee | [Ligation of pBAD and OmpA](/Team%3AMinnesota/leex4462-04232011000000-35 "Team:Minnesota/leex4462-04232011000000-35") |

### Date: Tuesday, 26 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 26 Apr 2011 00:00:00 GMT | Sukdong Lee | [Restriction digest of OmpA, PCR of OmpA (back-up)](/Team%3AMinnesota/leex4462-04262011000000-36 "Team:Minnesota/leex4462-04262011000000-36") |
| Tue, 26 Apr 2011 15:52:22 GMT | Viki Ding | [PCR set up](/Team%3AMinnesota/dingx055-04262011155222-153 "Team:Minnesota/dingx055-04262011155222-153") |
| Tue, 26 Apr 2011 16:02:53 GMT | Viki Ding | [PCR trial #2](/Team%3AMinnesota/dingx055-04262011160253-154 "Team:Minnesota/dingx055-04262011160253-154") |

### Date: Thursday, 28 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 28 Apr 2011 00:00:00 GMT | Sukdong Lee | [PCR of OmpA and Gel](/Team%3AMinnesota/leex4462-04282011000000-37 "Team:Minnesota/leex4462-04282011000000-37") |

### Date: Saturday, 30 April 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sat, 30 Apr 2011 00:00:00 GMT | Sukdong Lee | [Speed Vacuum](/Team%3AMinnesota/leex4462-04302011000000-38 "Team:Minnesota/leex4462-04302011000000-38") |

### Date: Tuesday, 03 May 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 03 May 2011 00:00:00 GMT | Sukdong Lee | [Restriction Digest of pBAD and OmpA insert](/Team%3AMinnesota/leex4462-05032011000000-39 "Team:Minnesota/leex4462-05032011000000-39") |

### Date: Wednesday, 04 May 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 04 May 2011 00:00:00 GMT | Sukdong Lee | [PCR purification of OmpA and Gel Extraction](/Team%3AMinnesota/leex4462-05042011000000-41 "Team:Minnesota/leex4462-05042011000000-41") |

### Date: Thursday, 05 May 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 05 May 2011 00:00:00 GMT | Sukdong Lee | [Ligation of OmpA and pBAD](/Team%3AMinnesota/leex4462-05052011000000-42 "Team:Minnesota/leex4462-05052011000000-42") |

### Date: Friday, 06 May 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 06 May 2011 00:00:00 GMT | Sukdong Lee | [Transformation](/Team%3AMinnesota/leex4462-05062011000000-43 "Team:Minnesota/leex4462-05062011000000-43") |

### Date: Monday, 23 May 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 23 May 2011 00:00:00 GMT | Sukdong Lee | [Transformation and Liquid cultures of OmpA transformants](/Team%3AMinnesota/leex4462-05232011000000-44 "Team:Minnesota/leex4462-05232011000000-44") |

### Date: Tuesday, 24 May 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 24 May 2011 00:00:00 GMT | Sukdong Lee | [Miniprep and PCR, Liquid Cultures](/Team%3AMinnesota/leex4462-05242011000000-45 "Team:Minnesota/leex4462-05242011000000-45") |

### Date: Wednesday, 25 May 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 25 May 2011 00:00:00 GMT | Sukdong Lee | [Gel of OmpA Plasmid](/Team%3AMinnesota/leex4462-05252011000000-46 "Team:Minnesota/leex4462-05252011000000-46") |

### Date: Wednesday, 01 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 01 Jun 2011 00:00:00 GMT | Sukdong Lee | [Liquid cultures of pBAD plasmid](/Team%3AMinnesota/leex4462-06012011000000-47 "Team:Minnesota/leex4462-06012011000000-47") |

### Date: Thursday, 02 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 02 Jun 2011 00:00:00 GMT | Sukdong Lee | [PCR Amplification of OmpA and Miniprep of pBADBB](/Team%3AMinnesota/leex4462-06022011000000-48 "Team:Minnesota/leex4462-06022011000000-48") |

### Date: Friday, 03 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 03 Jun 2011 00:00:00 GMT | Sukdong Lee | [Gel Electrophoresis of OmpA and pBAD PCR Products and Digests](/Team%3AMinnesota/leex4462-06032011000000-49 "Team:Minnesota/leex4462-06032011000000-49") |
| Fri, 03 Jun 2011 00:01:00 GMT | Sukdong Lee | [Extractions of OmpA](/Team%3AMinnesota/leex4462-06032011000100-50 "Team:Minnesota/leex4462-06032011000100-50") |

### Date: Sunday, 05 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sun, 05 Jun 2011 00:00:00 GMT | Sukdong Lee | [Speed Vac and Digests](/Team%3AMinnesota/leex4462-06052011000000-51 "Team:Minnesota/leex4462-06052011000000-51") |

### Date: Monday, 06 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 06 Jun 2011 00:00:00 GMT | Sukdong Lee | [Gel and Extraction](/Team%3AMinnesota/leex4462-06062011000000-52 "Team:Minnesota/leex4462-06062011000000-52") |

### Date: Tuesday, 07 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 07 Jun 2011 00:00:00 GMT | Sukdong Lee | [Digestion of OmpA](/Team%3AMinnesota/leex4462-06072011000000-53 "Team:Minnesota/leex4462-06072011000000-53") |

### Date: Wednesday, 08 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 08 Jun 2011 00:00:00 GMT | Sukdong Lee | [PCR Purification/Gel Extraction](/Team%3AMinnesota/leex4462-06082011000000-54 "Team:Minnesota/leex4462-06082011000000-54") |

### Date: Thursday, 09 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 09 Jun 2011 00:00:00 GMT | Sukdong Lee | [Ligation of OmpA and pBAD](/Team%3AMinnesota/leex4462-06092011000000-55 "Team:Minnesota/leex4462-06092011000000-55") |

### Date: Friday, 10 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 10 Jun 2011 00:00:00 GMT | Sukdong Lee | [Transformation of OmpA plasmid](/Team%3AMinnesota/leex4462-06102011000000-56 "Team:Minnesota/leex4462-06102011000000-56") |

### Date: Sunday, 12 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sun, 12 Jun 2011 00:00:00 GMT | Sukdong Lee | [Electroporation transformation](/Team%3AMinnesota/leex4462-06122011000000-57 "Team:Minnesota/leex4462-06122011000000-57") |

### Date: Monday, 13 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 13 Jun 2011 00:00:00 GMT | Sukdong Lee | [Ligation of OmpA+pBAD](/Team%3AMinnesota/leex4462-06132011000000-58 "Team:Minnesota/leex4462-06132011000000-58") |

### Date: Tuesday, 14 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 14 Jun 2011 00:00:00 GMT | Sukdong Lee | [Ligation](/Team%3AMinnesota/leex4462-06142011000000-59 "Team:Minnesota/leex4462-06142011000000-59") |

### Date: Wednesday, 15 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 15 Jun 2011 00:00:00 GMT | Sukdong Lee | [Electroporation Transformation](/Team%3AMinnesota/leex4462-06152011000000-60 "Team:Minnesota/leex4462-06152011000000-60") |

### Date: Saturday, 18 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sat, 18 Jun 2011 00:00:00 GMT | Sukdong Lee | [PCR of OmpA](/Team%3AMinnesota/leex4462-06182011000000-61 "Team:Minnesota/leex4462-06182011000000-61") |

### Date: Monday, 20 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 20 Jun 2011 00:00:00 GMT | Sukdong Lee | [TOPO Cloning and Gel Extraction](/Team%3AMinnesota/leex4462-06202011000000-62 "Team:Minnesota/leex4462-06202011000000-62") |

### Date: Tuesday, 21 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 21 Jun 2011 00:00:00 GMT | Sukdong Lee | [Liquid Cultures of TOPO Cloning](/Team%3AMinnesota/leex4462-06212011000000-63 "Team:Minnesota/leex4462-06212011000000-63") |

### Date: Wednesday, 22 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 22 Jun 2011 00:00:00 GMT | Sukdong Lee | [GoGreen PCR and Miniprep of TOPO Liquid Cultures](/Team%3AMinnesota/leex4462-06222011000000-64 "Team:Minnesota/leex4462-06222011000000-64") |

### Date: Thursday, 23 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 23 Jun 2011 00:00:00 GMT | Sukdong Lee | [Restriction Digests](/Team%3AMinnesota/leex4462-06232011000000-65 "Team:Minnesota/leex4462-06232011000000-65") |

### Date: Friday, 24 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 24 Jun 2011 00:00:00 GMT | Sukdong Lee | [Gel Extraction and Ligation](/Team%3AMinnesota/leex4462-06242011000000-66 "Team:Minnesota/leex4462-06242011000000-66") |

### Date: Saturday, 25 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sat, 25 Jun 2011 00:00:00 GMT | Sukdong Lee | [Electroporation](/Team%3AMinnesota/leex4462-06252011000000-67 "Team:Minnesota/leex4462-06252011000000-67") |
| Sat, 25 Jun 2011 00:01:00 GMT | Sukdong Lee | [Chemical Transformation](/Team%3AMinnesota/leex4462-06252011000100-68 "Team:Minnesota/leex4462-06252011000100-68") |

### Date: Sunday, 26 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sun, 26 Jun 2011 00:00:00 GMT | Sukdong Lee | [Liquid cultures of OmpA plasmids](/Team%3AMinnesota/leex4462-06262011000000-69 "Team:Minnesota/leex4462-06262011000000-69") |

### Date: Monday, 27 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 27 Jun 2011 00:00:00 GMT | Sukdong Lee | [Miniprep of OmpA and pBAD plasmid](/Team%3AMinnesota/leex4462-06272011000000-70 "Team:Minnesota/leex4462-06272011000000-70") |

### Date: Tuesday, 28 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 28 Jun 2011 00:00:00 GMT | Sukdong Lee | [Gogreen PCR and Digestion Check](/Team%3AMinnesota/leex4462-06282011000000-71 "Team:Minnesota/leex4462-06282011000000-71") |
| Tue, 28 Jun 2011 16:10:19 GMT | Viki Ding | [PCR of INP](/Team%3AMinnesota/dingx055-06282011161019-155 "Team:Minnesota/dingx055-06282011161019-155") |

### Date: Wednesday, 29 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 29 Jun 2011 00:00:00 GMT | Sukdong Lee | [Silicatein: Digest of pucBB](/Team%3AMinnesota/leex4462-06292011000000-73 "Team:Minnesota/leex4462-06292011000000-73") |
| Wed, 29 Jun 2011 00:01:00 GMT | Sukdong Lee | [Ligation of pucBB plasmid and Silicatein](/Team%3AMinnesota/leex4462-06292011000100-74 "Team:Minnesota/leex4462-06292011000100-74") |

### Date: Thursday, 30 June 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 30 Jun 2011 00:00:00 GMT | Sukdong Lee | [Transformation](/Team%3AMinnesota/leex4462-06302011000000-75 "Team:Minnesota/leex4462-06302011000000-75") |

### Date: Friday, 01 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 01 Jul 2011 00:00:00 GMT | Sukdong Lee | [Liquid cultures of pucBB+Silicatein Transformants](/Team%3AMinnesota/leex4462-07012011000000-76 "Team:Minnesota/leex4462-07012011000000-76") |

### Date: Saturday, 02 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sat, 02 Jul 2011 00:00:00 GMT | Sukdong Lee | [Miniprep of Silicatein+pucBB transformants](/Team%3AMinnesota/leex4462-07022011000000-77 "Team:Minnesota/leex4462-07022011000000-77") |

### Date: Monday, 04 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 04 Jul 2011 00:00:00 GMT | Sukdong Lee | [Liquid Gogreen PCR](/Team%3AMinnesota/leex4462-07042011000000-78 "Team:Minnesota/leex4462-07042011000000-78") |

### Date: Wednesday, 06 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 06 Jul 2011 16:15:53 GMT | Viki Ding | [INP PCR clean up](/Team%3AMinnesota/dingx055-07062011161553-156 "Team:Minnesota/dingx055-07062011161553-156") |

### Date: Thursday, 07 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 07 Jul 2011 00:00:00 GMT | Sukdong Lee | [Gogreen of Silicatein pucBB plasmid](/Team%3AMinnesota/leex4462-07072011000000-79 "Team:Minnesota/leex4462-07072011000000-79") |
| Thu, 07 Jul 2011 00:01:00 GMT | Sukdong Lee | [Restriction Digest of pucBB and PCR of Silicatein](/Team%3AMinnesota/leex4462-07072011000100-80 "Team:Minnesota/leex4462-07072011000100-80") |

### Date: Friday, 08 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 08 Jul 2011 00:00:00 GMT | Sukdong Lee | [Gel extraction of Silicatein, Liquid Culture of pucBB, and Ligation](/Team%3AMinnesota/leex4462-07082011000000-81 "Team:Minnesota/leex4462-07082011000000-81") |

### Date: Monday, 11 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 11 Jul 2011 00:00:00 GMT | Sukdong Lee | [Gel Extraction of Silicatein and Liquid Cultures of pucBB](/Team%3AMinnesota/leex4462-07112011000000-82 "Team:Minnesota/leex4462-07112011000000-82") |

### Date: Tuesday, 12 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 12 Jul 2011 00:00:00 GMT | Sukdong Lee | [Transformation of pucBB+Silicatein](/Team%3AMinnesota/leex4462-07122011000000-83 "Team:Minnesota/leex4462-07122011000000-83") |
| Tue, 12 Jul 2011 16:17:17 GMT | Viki Ding | [Digestion of INP](/Team%3AMinnesota/dingx055-07122011161717-157 "Team:Minnesota/dingx055-07122011161717-157") |

### Date: Wednesday, 13 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 13 Jul 2011 00:00:00 GMT | Sukdong Lee | [Gogreen PCR and Miniprep](/Team%3AMinnesota/leex4462-07132011000000-84 "Team:Minnesota/leex4462-07132011000000-84") |
| Wed, 13 Jul 2011 16:20:04 GMT | Viki Ding | [INP cloning](/Team%3AMinnesota/dingx055-07132011162004-158 "Team:Minnesota/dingx055-07132011162004-158") |

### Date: Friday, 15 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 15 Jul 2011 00:00:00 GMT | Sukdong Lee | [pucBB Digest and pucBB Liquid Cultures](/Team%3AMinnesota/leex4462-07152011000000-85 "Team:Minnesota/leex4462-07152011000000-85") |

### Date: Saturday, 16 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sat, 16 Jul 2011 00:00:00 GMT | Sukdong Lee | [Miniprep of pucBB and gel extraction of pucBB](/Team%3AMinnesota/leex4462-07162011000000-86 "Team:Minnesota/leex4462-07162011000000-86") |

### Date: Wednesday, 20 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 20 Jul 2011 00:00:00 GMT | Sukdong Lee | [PCR of Silicatein, Gel Extraction, and TOPO Cloning](/Team%3AMinnesota/leex4462-07202011000000-87 "Team:Minnesota/leex4462-07202011000000-87") |
| Wed, 20 Jul 2011 00:01:00 GMT | Sukdong Lee | [TOPO Transformants and Liquid Cultures](/Team%3AMinnesota/leex4462-07202011000100-88 "Team:Minnesota/leex4462-07202011000100-88") |
| Wed, 20 Jul 2011 17:59:22 GMT | Viki Ding | [Transformation trial #2](/Team%3AMinnesota/dingx055-07202011175922-159 "Team:Minnesota/dingx055-07202011175922-159") |

### Date: Friday, 22 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 22 Jul 2011 00:00:00 GMT | Sukdong Lee | [TOPO Miniprep and Gogreen PCR and Gel](/Team%3AMinnesota/leex4462-07222011000000-89 "Team:Minnesota/leex4462-07222011000000-89") |

### Date: Monday, 25 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 25 Jul 2011 00:00:00 GMT | Sukdong Lee | [Gogreen PCR X3 and Gel X3](/Team%3AMinnesota/leex4462-07252011000000-90 "Team:Minnesota/leex4462-07252011000000-90") |

### Date: Tuesday, 26 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 26 Jul 2011 00:00:00 GMT | Sukdong Lee | [PCR of Silicatein and TOPO](/Team%3AMinnesota/leex4462-07262011000000-91 "Team:Minnesota/leex4462-07262011000000-91") |
| Tue, 26 Jul 2011 18:02:01 GMT | Viki Ding | [INP PCR with Taq mix](/Team%3AMinnesota/dingx055-07262011180201-160 "Team:Minnesota/dingx055-07262011180201-160") |

### Date: Wednesday, 27 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 27 Jul 2011 00:00:00 GMT | Sukdong Lee | [Liquid Cultures and Colony Gogreen PCR](/Team%3AMinnesota/leex4462-07272011000000-92 "Team:Minnesota/leex4462-07272011000000-92") |
| Wed, 27 Jul 2011 18:06:41 GMT | Viki Ding | [INP PCR w/Taq & digestion](/Team%3AMinnesota/dingx055-07272011180641-161 "Team:Minnesota/dingx055-07272011180641-161") |

### Date: Thursday, 28 July 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 28 Jul 2011 00:00:00 GMT | Sukdong Lee | [Miniprep and Sequencing of the TOPO Silicatein](/Team%3AMinnesota/leex4462-07282011000000-93 "Team:Minnesota/leex4462-07282011000000-93") |

### Date: Tuesday, 02 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 02 Aug 2011 18:12:35 GMT | Viki Ding | [Ligation & transformation](/Team%3AMinnesota/dingx055-08022011181235-162 "Team:Minnesota/dingx055-08022011181235-162") |

### Date: Wednesday, 03 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 03 Aug 2011 00:00:00 GMT | Sukdong Lee | [Digestion of Silicatein TOPO 7](/Team%3AMinnesota/leex4462-08032011000000-94 "Team:Minnesota/leex4462-08032011000000-94") |

### Date: Thursday, 04 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 04 Aug 2011 00:00:00 GMT | Sukdong Lee | [Gel Extraction, Ligation, and Transformation](/Team%3AMinnesota/leex4462-08042011000000-95 "Team:Minnesota/leex4462-08042011000000-95") |

### Date: Monday, 08 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 08 Aug 2011 00:00:00 GMT | Sukdong Lee | [Liquid Cultures, Master Plate, Gogreen, and Gel](/Team%3AMinnesota/leex4462-08082011000000-96 "Team:Minnesota/leex4462-08082011000000-96") |

### Date: Tuesday, 09 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 09 Aug 2011 00:00:00 GMT | Sukdong Lee | [Miniprep, Gogreen Liquid PCR, and Gel](/Team%3AMinnesota/leex4462-08092011000000-97 "Team:Minnesota/leex4462-08092011000000-97") |

### Date: Wednesday, 10 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 10 Aug 2011 00:00:00 GMT | Sukdong Lee | [Liquid Cultures, Liquid Gogreen PCR](/Team%3AMinnesota/leex4462-08102011000000-98 "Team:Minnesota/leex4462-08102011000000-98") |

### Date: Thursday, 11 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 11 Aug 2011 00:00:00 GMT | Sukdong Lee | [More Liquid Cultures, Liquid Gogreen PCR](/Team%3AMinnesota/leex4462-08112011000000-99 "Team:Minnesota/leex4462-08112011000000-99") |

### Date: Friday, 12 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 12 Aug 2011 00:00:00 GMT | Sukdong Lee | [Transformation of TOPO Silicatein Back-up](/Team%3AMinnesota/leex4462-08122011000000-100 "Team:Minnesota/leex4462-08122011000000-100") |

### Date: Saturday, 13 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sat, 13 Aug 2011 00:00:00 GMT | Sukdong Lee | [Liquid Cultures of Back-Up Silicatein TOPO](/Team%3AMinnesota/leex4462-08132011000000-101 "Team:Minnesota/leex4462-08132011000000-101") |

### Date: Sunday, 14 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sun, 14 Aug 2011 00:00:00 GMT | Sukdong Lee | [Miniprep of Silicatein TOPO](/Team%3AMinnesota/leex4462-08142011000000-102 "Team:Minnesota/leex4462-08142011000000-102") |

### Date: Monday, 15 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 15 Aug 2011 00:00:00 GMT | Sukdong Lee | [Digest of pucBB and Silicatein TOPO](/Team%3AMinnesota/leex4462-08152011000000-103 "Team:Minnesota/leex4462-08152011000000-103") |

### Date: Tuesday, 16 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 16 Aug 2011 00:00:00 GMT | Sukdong Lee | [Miniprep of pucBB and Gel Extraction](/Team%3AMinnesota/leex4462-08162011000000-104 "Team:Minnesota/leex4462-08162011000000-104") |
| Tue, 16 Aug 2011 18:14:41 GMT | Viki Ding | [Digestion](/Team%3AMinnesota/dingx055-08162011181441-163 "Team:Minnesota/dingx055-08162011181441-163") |

### Date: Wednesday, 17 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 17 Aug 2011 00:00:00 GMT | Sukdong Lee | [Liquid Cultures of Transformants and Gogreen](/Team%3AMinnesota/leex4462-08172011000000-105 "Team:Minnesota/leex4462-08172011000000-105") |

### Date: Thursday, 18 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 18 Aug 2011 00:00:00 GMT | Sukdong Lee | [Gogreen PCR of Liquid Cultures](/Team%3AMinnesota/leex4462-08182011000000-106 "Team:Minnesota/leex4462-08182011000000-106") |

### Date: Friday, 19 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 19 Aug 2011 00:00:00 GMT | Sukdong Lee | [Gogreen PCR of Liquid Cultures](/Team%3AMinnesota/leex4462-08192011000000-107 "Team:Minnesota/leex4462-08192011000000-107") |

### Date: Monday, 22 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 22 Aug 2011 00:00:00 GMT | Sukdong Lee | [Ligation and Transformation](/Team%3AMinnesota/leex4462-08222011000000-108 "Team:Minnesota/leex4462-08222011000000-108") |
| Mon, 22 Aug 2011 10:15:36 GMT | Sukdong Lee | [Minnesota/leex4462-08222011101536-34](/Team%3AMinnesota/leex4462-08222011101536-34 "Team:Minnesota/leex4462-08222011101536-34") |

### Date: Tuesday, 23 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 23 Aug 2011 00:00:00 GMT | Sukdong Lee | [Ligation of pBAD+Silicatein and Digest of TOPO Silicatein](/Team%3AMinnesota/leex4462-08232011000000-109 "Team:Minnesota/leex4462-08232011000000-109") |
| Tue, 23 Aug 2011 00:00:00 GMT | Sukdong Lee | [Liquid Cultures, Gogreen PCR of Silicatein+pBAD](/Team%3AMinnesota/leex4462-08232011000000-110 "Team:Minnesota/leex4462-08232011000000-110") |

### Date: Wednesday, 24 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 24 Aug 2011 00:00:00 GMT | Sukdong Lee | [pBAD+Silicatein transformation and gel extraction of TOPO Silicatein](/Team%3AMinnesota/leex4462-08242011000000-111 "Team:Minnesota/leex4462-08242011000000-111") |
| Wed, 24 Aug 2011 00:00:00 GMT | Sukdong Lee | [Ligation, Digest, Transformation of pBAD](/Team%3AMinnesota/leex4462-08242011000000-123 "Team:Minnesota/leex4462-08242011000000-123") |

### Date: Thursday, 25 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 25 Aug 2011 00:00:00 GMT | Sukdong Lee | [Liquid Cultures, Gogreen PCR of Silicatein+pBAD](/Team%3AMinnesota/leex4462-08252011000000-112 "Team:Minnesota/leex4462-08252011000000-112") |
| Thu, 25 Aug 2011 00:00:00 GMT | Sukdong Lee | [Minnesota/leex4462-08252011000000-124](/Team%3AMinnesota/leex4462-08252011000000-124 "Team:Minnesota/leex4462-08252011000000-124") |
| Thu, 25 Aug 2011 18:25:40 GMT | Viki Ding | [Cloning #3](/Team%3AMinnesota/dingx055-08252011182540-165 "Team:Minnesota/dingx055-08252011182540-165") |

### Date: Friday, 26 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 26 Aug 2011 00:00:00 GMT | Sukdong Lee | [Miniprep and Gogreen Check](/Team%3AMinnesota/leex4462-08262011000000-113 "Team:Minnesota/leex4462-08262011000000-113") |
| Fri, 26 Aug 2011 00:00:00 GMT | Sukdong Lee | [Minnesota/leex4462-08262011000000-125](/Team%3AMinnesota/leex4462-08262011000000-125 "Team:Minnesota/leex4462-08262011000000-125") |

### Date: Saturday, 27 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sat, 27 Aug 2011 18:17:00 GMT | Viki Ding | [Cloning](/Team%3AMinnesota/dingx055-08272011181700-164 "Team:Minnesota/dingx055-08272011181700-164") |

### Date: Monday, 29 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 29 Aug 2011 00:00:00 GMT | Sukdong Lee | [Ligation, Digest, Transformation of pBAD](/Team%3AMinnesota/leex4462-08292011000000-126 "Team:Minnesota/leex4462-08292011000000-126") |
| Mon, 29 Aug 2011 18:30:52 GMT | Viki Ding | [Verifying clones](/Team%3AMinnesota/dingx055-08292011183052-166 "Team:Minnesota/dingx055-08292011183052-166") |

### Date: Tuesday, 30 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 30 Aug 2011 00:00:00 GMT | Sukdong Lee | [Ligation, Gel Extraction, Miniprep, Liquid Cultures of pBAD, Transformation](/Team%3AMinnesota/leex4462-08302011000000-127 "Team:Minnesota/leex4462-08302011000000-127") |
| Tue, 30 Aug 2011 18:38:12 GMT | Viki Ding | [SiL & GFP PCT](/Team%3AMinnesota/dingx055-08302011183812-167 "Team:Minnesota/dingx055-08302011183812-167") |

### Date: Wednesday, 31 August 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 31 Aug 2011 00:00:00 GMT | Sukdong Lee | [Miniprep of pBAD](/Team%3AMinnesota/leex4462-08312011000000-128 "Team:Minnesota/leex4462-08312011000000-128") |

### Date: Friday, 02 September 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 02 Sep 2011 00:00:00 GMT | Sukdong Lee | [PCR Overlap Extension (OmpA+Silicatein Fusion)](/Team%3AMinnesota/leex4462-09022011000000-129 "Team:Minnesota/leex4462-09022011000000-129") |
| Fri, 02 Sep 2011 00:01:00 GMT | Sukdong Lee | [Gel of PCR Overlap](/Team%3AMinnesota/leex4462-09022011000100-130 "Team:Minnesota/leex4462-09022011000100-130") |
| Fri, 02 Sep 2011 00:02:00 GMT | Sukdong Lee | [PCR Overlap Test](/Team%3AMinnesota/leex4462-09022011000200-131 "Team:Minnesota/leex4462-09022011000200-131") |

### Date: Tuesday, 06 September 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 06 Sep 2011 00:00:00 GMT | Sukdong Lee | [Gel of PCR Overlap](/Team%3AMinnesota/leex4462-09062011000000-132 "Team:Minnesota/leex4462-09062011000000-132") |
| Tue, 06 Sep 2011 00:01:00 GMT | Sukdong Lee | [PCR Overlap Extension](/Team%3AMinnesota/leex4462-09062011000100-133 "Team:Minnesota/leex4462-09062011000100-133") |
| Tue, 06 Sep 2011 18:47:17 GMT | Viki Ding | [Further cloning](/Team%3AMinnesota/dingx055-09062011184717-168 "Team:Minnesota/dingx055-09062011184717-168") |

### Date: Wednesday, 07 September 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 07 Sep 2011 00:00:00 GMT | Sukdong Lee | [Gel of PCR Products/Gel Extraction](/Team%3AMinnesota/leex4462-09072011000000-134 "Team:Minnesota/leex4462-09072011000000-134") |

### Date: Thursday, 08 September 2011

| Date | Author | Subject |
| --- | --- | --- |
| Thu, 08 Sep 2011 00:00:00 GMT | Sukdong Lee | [PCR Overlap Extension](/Team%3AMinnesota/leex4462-09082011000000-135 "Team:Minnesota/leex4462-09082011000000-135") |
| Thu, 08 Sep 2011 00:01:00 GMT | Sukdong Lee | [Gel Extraction of the PCR Overlap Extension](/Team%3AMinnesota/leex4462-09082011000100-136 "Team:Minnesota/leex4462-09082011000100-136") |
| Thu, 08 Sep 2011 00:03:00 GMT | Sukdong Lee | [Transformation of pBAD+ OmpA](/Team%3AMinnesota/leex4462-09082011000300-137 "Team:Minnesota/leex4462-09082011000300-137") |
| Thu, 08 Sep 2011 00:04:00 GMT | Sukdong Lee | [PCR of Silicatein](/Team%3AMinnesota/leex4462-09082011000400-138 "Team:Minnesota/leex4462-09082011000400-138") |
| Thu, 08 Sep 2011 18:51:05 GMT | Viki Ding | [More cloning](/Team%3AMinnesota/dingx055-09082011185105-169 "Team:Minnesota/dingx055-09082011185105-169") |

### Date: Friday, 09 September 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 09 Sep 2011 00:00:00 GMT | Sukdong Lee | [Part II of PCR Overlap](/Team%3AMinnesota/leex4462-09092011000000-139 "Team:Minnesota/leex4462-09092011000000-139") |
| Fri, 09 Sep 2011 00:01:00 GMT | Sukdong Lee | [Liquid Cultures of pBAD and pBAD+OmpA](/Team%3AMinnesota/leex4462-09092011000100-140 "Team:Minnesota/leex4462-09092011000100-140") |
| Fri, 09 Sep 2011 00:02:00 GMT | Sukdong Lee | [PCR of Silicatein](/Team%3AMinnesota/leex4462-09092011000200-141 "Team:Minnesota/leex4462-09092011000200-141") |
| Fri, 09 Sep 2011 00:03:00 GMT | Sukdong Lee | [Gel of Part2+3 and Test of Part 3](/Team%3AMinnesota/leex4462-09092011000300-142 "Team:Minnesota/leex4462-09092011000300-142") |

### Date: Saturday, 10 September 2011

| Date | Author | Subject |
| --- | --- | --- |
| Sat, 10 Sep 2011 00:00:00 GMT | Sukdong Lee | [Miniprep of OmpA+pBAD and pBAD](/Team%3AMinnesota/leex4462-09102011000000-143 "Team:Minnesota/leex4462-09102011000000-143") |
| Sat, 10 Sep 2011 00:01:00 GMT | Sukdong Lee | [Gel Extraction of Part 3](/Team%3AMinnesota/leex4462-09102011000100-144 "Team:Minnesota/leex4462-09102011000100-144") |
| Sat, 10 Sep 2011 00:03:00 GMT | Sukdong Lee | [Gel of PCR Silicatein](/Team%3AMinnesota/leex4462-09102011000300-145 "Team:Minnesota/leex4462-09102011000300-145") |
| Sat, 10 Sep 2011 00:04:00 GMT | Sukdong Lee | [PCR Overlap Check](/Team%3AMinnesota/leex4462-09102011000400-146 "Team:Minnesota/leex4462-09102011000400-146") |

### Date: Monday, 12 September 2011

| Date | Author | Subject |
| --- | --- | --- |
| Mon, 12 Sep 2011 00:00:00 GMT | Sukdong Lee | [Gel of PCR Products](/Team%3AMinnesota/leex4462-09122011000000-147 "Team:Minnesota/leex4462-09122011000000-147") |
| Mon, 12 Sep 2011 00:01:00 GMT | Sukdong Lee | [PCR of Part 3 and Silicatein](/Team%3AMinnesota/leex4462-09122011000100-148 "Team:Minnesota/leex4462-09122011000100-148") |
| Mon, 12 Sep 2011 18:54:16 GMT | Viki Ding | [Verifying clones](/Team%3AMinnesota/dingx055-09122011185416-170 "Team:Minnesota/dingx055-09122011185416-170") |

### Date: Tuesday, 13 September 2011

| Date | Author | Subject |
| --- | --- | --- |
| Tue, 13 Sep 2011 00:00:00 GMT | Sukdong Lee | [PCR of Part 3](/Team%3AMinnesota/leex4462-09132011000000-149 "Team:Minnesota/leex4462-09132011000000-149") |

### Date: Wednesday, 14 September 2011

| Date | Author | Subject |
| --- | --- | --- |
| Wed, 14 Sep 2011 00:00:00 GMT | Sukdong Lee | [Gel of PCR](/Team%3AMinnesota/leex4462-09142011000000-150 "Team:Minnesota/leex4462-09142011000000-150") |

### Date: Friday, 16 September 2011

| Date | Author | Subject |
| --- | --- | --- |
| Fri, 16 Sep 2011 00:00:00 GMT | Sukdong Lee | [PCR of Silicatein, part 1, part S, part 2, and part 3](/Team%3AMinnesota/leex4462-09162011000000-151 "Team:Minnesota/leex4462-09162011000000-151") |
| Fri, 16 Sep 2011 00:01:00 GMT | Sukdong Lee | [Gel of PCR Products+Extraction](/Team%3AMinnesota/leex4462-09162011000100-152 "Team:Minnesota/leex4462-09162011000100-152") |

---

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