iGEM 2020 — Team Qdai

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:Qdai/Notebook

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# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:Qdai/Engineering

Document

**Team:Qdai**

* [Home](https://2020.igem.org/Team%3AQdai)
* Team

  [Members](https://2020.igem.org/Team%3AQdai/Members)
  [Attributions](https://2020.igem.org/Team%3AQdai/Attributions)
  [Sponsors](https://2020.igem.org/Team%3AQdai/Sponsors)
* Project

  [Description](https://2020.igem.org/Team%3AQdai/Description)
  [Design](https://2020.igem.org/Team%3AQdai/Design)
  [Model](https://2020.igem.org/Team%3AQdai/Model)

  [Contribution](https://2020.igem.org/Team%3AQdai/Contribution)
  [Engineering](https://2020.igem.org/Team%3AQdai/Engineering)
* Lab

  [Experiments](https://2020.igem.org/Team%3AQdai/Experiments)
  [Parts](https://2020.igem.org/Team%3AQdai/Parts)

  [Safety](https://2020.igem.org/Team%3AQdai/Safety)
* Human Practice

  [Intergrated Human Practice](https://2020.igem.org/Team%3AQdai/Human_Practices)
  [Science Communication](https://2020.igem.org/Team%3AQdai/Education)
  [Collaboration](https://2020.igem.org/Team%3AQdai/Collaborations)
  [Proposed Implementation](https://2020.igem.org/Team%3AQdai/Implementation)
* [FeedBack](https://2020.igem.org/Team%3AQdai/Feedback)

Document

## Engineering

### Engineering Success

We have not been able to perform experiments because access to the laboratory was almost impossible until recently due to COVID-19. Therefore, we assume that the experiments will be carried out when access to the laboratory is available.

We plan to introduce three genes, *ppk*, *relA*, and *phnCDE*. Based on our previous research, they may not lead to the accumulation of polyphosphate as desired.

### ppk

*ppk* uses ATP to synthesize polyphosphate. If we introduce *ppk* into a plasmid with high expression, ATP, which is necessary to maintain the organism, is only used up for the synthesis of polyphosphate, and the growth of *E. coli* itself may be significantly inhibited. It will be possible to estimate the impact of *ppk* on growth by changing the expression levels of plasmids containing only *ppk* and observing their variation.

### phnCDE

*phnCDE* is a transporter of phosphoric acid, but since the lyase is located next to the transporter, it is considered to transport the organic phosphorus mainly. Therefore, if only orthophosphate is added when creating a phosphorus-rich environment, it may not be very effective. The effect of *phnCDE* should be properly reflected by adding organophosphorus in the environment.

### Dual Plasmid System

By introducing *ppk* and *relA* into different plasmids using promoters pLlacO1 and pLtetO1 to create a dual plasmid system. The advantage of the system is that induction can be performed separately, allowing us to compare results to the modeling equations, making it easier to improve them.

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:Qdai/Experiments

Document

**Team:Qdai**

* [Home](https://2020.igem.org/Team%3AQdai)
* Team

  [Members](https://2020.igem.org/Team%3AQdai/Members)
  [Attributions](https://2020.igem.org/Team%3AQdai/Attributions)
  [Sponsors](https://2020.igem.org/Team%3AQdai/Sponsors)
* Project

  [Description](https://2020.igem.org/Team%3AQdai/Description)
  [Design](https://2020.igem.org/Team%3AQdai/Design)
  [Model](https://2020.igem.org/Team%3AQdai/Model)

  [Contribution](https://2020.igem.org/Team%3AQdai/Contribution)
  [Engineering](https://2020.igem.org/Team%3AQdai/Engineering)
* Lab

  [Experiments](https://2020.igem.org/Team%3AQdai/Experiments)
  [Parts](https://2020.igem.org/Team%3AQdai/Parts)

  [Safety](https://2020.igem.org/Team%3AQdai/Safety)
* Human Practice

  [Intergrated Human Practice](https://2020.igem.org/Team%3AQdai/Human_Practices)
  [Science Communication](https://2020.igem.org/Team%3AQdai/Education)
  [Collaboration](https://2020.igem.org/Team%3AQdai/Collaborations)
  [Proposed Implementation](https://2020.igem.org/Team%3AQdai/Implementation)
* [FeedBack](https://2020.igem.org/Team%3AQdai/Feedback)

Document

## Experiments

Our lab has been closed this season due to the spread of the Covid-19. We are looking forward to constructing plasmids we designed!

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:Qdai/Results

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# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)