iGEM 2018 — Team NUDT_CHINA

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:NUDT CHINA/Notebook

![](https://static.igem.org/mediawiki/2018/b/b4/T--NUDT_CHINA--mainpage-top_left_corner.png)

* [HOME](https://2018.igem.org/Team%3ANUDT_CHINA)
* TEAM
  + [Team Members](https://2018.igem.org/Team%3ANUDT_CHINA/Team)
  + [Collaborations](https://2018.igem.org/Team%3ANUDT_CHINA/Collaborations)
* PROJECT
  + [Description](https://2018.igem.org/Team%3ANUDT_CHINA/Description)
  + [Design](https://2018.igem.org/Team%3ANUDT_CHINA/Design)
  + [Experiments](https://2018.igem.org/Team%3ANUDT_CHINA/Experiments)
  + [Notebook](https://2018.igem.org/Team%3ANUDT_CHINA/Notebook)
  + [InterLab](https://2018.igem.org/Team%3ANUDT_CHINA/InterLab)
  + [Results](https://2018.igem.org/Team%3ANUDT_CHINA/Results)
  + [Demonstrate](https://2018.igem.org/Team%3ANUDT_CHINA/Demonstrate)
  + [Improve](https://2018.igem.org/Team%3ANUDT_CHINA/Improve)
  + [Attributions](https://2018.igem.org/Team%3ANUDT_CHINA/Attributions)
* MODEL
  + [Model](https://2018.igem.org/Team%3ANUDT_CHINA/Model)
  + [Overview](https://2018.igem.org/Team%3ANUDT_CHINA/Model/Overview)
  + [Basic Design](https://2018.igem.org/Team%3ANUDT_CHINA/Model/Basic%20Design)
  + [Deduction](https://2018.igem.org/Team%3ANUDT_CHINA/Model/Deduction)
  + [Discussion](https://2018.igem.org/Team%3ANUDT_CHINA/Model/Discussion)
* PARTS
  + [Parts Overview](https://2018.igem.org/Team%3ANUDT_CHINA/Parts)
  + [Basic Parts](https://2018.igem.org/Team%3ANUDT_CHINA/Basic_Part)
  + [Composite Parts](https://2018.igem.org/Team%3ANUDT_CHINA/Composite_Part)
  + [Part Collection](https://2018.igem.org/Team%3ANUDT_CHINA/Part_Collection)
* [SAFETY](https://2018.igem.org/Team%3ANUDT_CHINA/Safety)
* Human Practices
  + [Human Practices](https://2018.igem.org/Team%3ANUDT_CHINA/Human_Practices)
  + [Education & Engagement](https://2018.igem.org/Team%3ANUDT_CHINA/Public_Engagement)
* AWARDS
  + [Model](https://2018.igem.org/Team%3ANUDT_CHINA/Model)

Designed Protein Degradation Method Based on

Trim21 And Nanobody              -- Notebook

#

Jiaxin Ma ,Tianyi Zhang,Chenyu Lu and Chenxing Sun completed most of the experiments.

Jiaxin Ma

|  |  |
| --- | --- |
| **D****ata** | **Project** |
| **6.15-7.3** | Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Tianyi Zhang. |
| **7.4-8.3** | Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Tianyi Zhang. |
| **8.4-8.15** | Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Tianyi Zhang. |
| **8.16-8.28** | Work on repeated validation of GFP interfering efficiency with Tianyi Zhang. |
| **8.29-9.22** | Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Tianyi Zhang. |
| **9.22-10.10** | Work on the proliferation and migration assay of cells with ErbB3 interfered with Tianyi Zhang. |

Tianyi Zhang

|  |  |
| --- | --- |
| **D****ata** | **Project** |
| **6.15-7.3** | Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Jiaxin Ma. |
| **7.4-8.3** | Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Jiaxin Ma. |
| **8.4-8.15** | Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Jiaxin Ma. |
| **8.16-8.28** | Work on repeated validation of GFP interfering efficiency with Jiaxin Ma. |
| **8.29-9.22** | Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Jiaxin Ma. |
| **9.22-10.10** | Work on the proliferation and migration assay of cells with ErbB3 interfered with Jiaxin Ma. |

Chenyu Lu

|  |  |
| --- | --- |
| **D****ata** | **Project** |
| **6.15-7.3** | Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenxing Sun. |
| **7.4-8.3** | Hela Cell culture and preparatory works on fluorescence detection with Chenxing Sun. |
| **8.4-8.28** | Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenxing Sun. |
| **8.29-9.22** | Construct Parts with Chenxing Sun. |
| **9.22-10.10** | Work on the experimental validation of parts with Chenxing Sun. |

Chenxing Sun

|  |  |
| --- | --- |
| **D****ata** | **Project** |
| **6.15-7.3** | Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenyu Lu . |
| **7.4-8.3** | Hela Cell culture and preparatory works on fluorescence detection with Chenyu Lu . |
| **8.4-8.28** | Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenyu Lu . |
| **8.29-9.22** | Construct Parts with Chenyu Lu . |
| **9.22-10.10** | Work on the experimental validation of parts with Chenyu Lu . |


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:NUDT CHINA/Experiments

![](https://static.igem.org/mediawiki/2018/b/b4/T--NUDT_CHINA--mainpage-top_left_corner.png)

* [HOME](https://2018.igem.org/Team%3ANUDT_CHINA)
* TEAM
  + [Team Members](https://2018.igem.org/Team%3ANUDT_CHINA/Team)
  + [Collaborations](https://2018.igem.org/Team%3ANUDT_CHINA/Collaborations)
* PROJECT
  + [Description](https://2018.igem.org/Team%3ANUDT_CHINA/Description)
  + [Design](https://2018.igem.org/Team%3ANUDT_CHINA/Design)
  + [Experiments](https://2018.igem.org/Team%3ANUDT_CHINA/Experiments)
  + [Notebook](https://2018.igem.org/Team%3ANUDT_CHINA/Notebook)
  + [InterLab](https://2018.igem.org/Team%3ANUDT_CHINA/InterLab)
  + [Results](https://2018.igem.org/Team%3ANUDT_CHINA/Results)
  + [Demonstrate](https://2018.igem.org/Team%3ANUDT_CHINA/Demonstrate)
  + [Improve](https://2018.igem.org/Team%3ANUDT_CHINA/Improve)
  + [Attributions](https://2018.igem.org/Team%3ANUDT_CHINA/Attributions)
* MODEL
  + [Model](https://2018.igem.org/Team%3ANUDT_CHINA/Model)
  + [Overview](https://2018.igem.org/Team%3ANUDT_CHINA/Model/Overview)
  + [Basic Design](https://2018.igem.org/Team%3ANUDT_CHINA/Model/Basic%20Design)
  + [Deduction](https://2018.igem.org/Team%3ANUDT_CHINA/Model/Deduction)
  + [Discussion](https://2018.igem.org/Team%3ANUDT_CHINA/Model/Discussion)
* PARTS
  + [Parts Overview](https://2018.igem.org/Team%3ANUDT_CHINA/Parts)
  + [Basic Parts](https://2018.igem.org/Team%3ANUDT_CHINA/Basic_Part)
  + [Composite Parts](https://2018.igem.org/Team%3ANUDT_CHINA/Composite_Part)
  + [Part Collection](https://2018.igem.org/Team%3ANUDT_CHINA/Part_Collection)
* [SAFETY](https://2018.igem.org/Team%3ANUDT_CHINA/Safety)
* Human Practices
  + [Human Practices](https://2018.igem.org/Team%3ANUDT_CHINA/Human_Practices)
  + [Education & Engagement](https://2018.igem.org/Team%3ANUDT_CHINA/Public_Engagement)
* AWARDS
  + [Model](https://2018.igem.org/Team%3ANUDT_CHINA/Model)

Designed Protein Degradation Method Based on

Trim21 And Nanobody              -- Experiments

[Protocol for Annealing](https://static.igem.org/mediawiki/2018/3/32/T--NUDT_CHINA---EXPERIMENT1.pdf)

[Protocol for Ligation](https://static.igem.org/mediawiki/2018/c/c8/T--NUDT_CHINA---EXPERIMENT3.pdf)

[Protocol for CCK8 (Cell Counting kit-8)](https://static.igem.org/mediawiki/2018/2/20/T--NUDT_CHINA--EXPERIMENT5.PDF)

[Protocol for Lipofectamine® 2000 Transfection](https://static.igem.org/mediawiki/2018/6/61/T--NUDT_CHINA--EXPERIMENT6.pdf)

[Protocol for Western blotting](https://static.igem.org/mediawiki/2018/3/3a/T--NUDT_CHINA--EXPERIMENT8.pdf)

[Protocol for cell culture and subculture](https://static.igem.org/mediawiki/2018/b/b7/T--NUDT_CHINA--EXPERIMENT9.pdf)

[Protocol for PCR with PrimeStar HS DNA polymerase](https://static.igem.org/mediawiki/2018/4/48/T--NUDT_CHINA--EXPERIMENT19.pdf)

[Protocol for Phosphorylation Modification](https://static.igem.org/mediawiki/2018/a/a2/T--NUDT_CHINA--EXPERIMENT13.pdf)

[Protocol for colony PCR](https://static.igem.org/mediawiki/2018/5/5a/T--NUDT_CHINA--EXPERIMENT20.pdf)

[Protocol for Agarose Gel Electrophoresis](https://static.igem.org/mediawiki/2018/4/45/T--NUDT_CHINA--EXPERIMENT21.PDF)

[Protocol for double digestion](https://static.igem.org/mediawiki/2018/1/1e/T--NUDT_CHINA--EXPERIMENT22.PDF)

[Protocol for Gel Purification with OMEGA Gel Extraction Kit](https://static.igem.org/mediawiki/2018/4/47/T--NUDT_CHINA--EXPERIMENT23.PDF)

[Protocol for mini-prep with OMEGA E.Z.N.A.® Plasmid DNA Mini Kit I](https://static.igem.org/mediawiki/2018/a/a7/T--NUDT_CHINA---EXPERIMENT25.pdf)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:NUDT CHINA/Results

![](https://static.igem.org/mediawiki/2018/b/b4/T--NUDT_CHINA--mainpage-top_left_corner.png)

* [HOME](https://2018.igem.org/Team%3ANUDT_CHINA)
* TEAM
  + [Team Members](https://2018.igem.org/Team%3ANUDT_CHINA/Team)
  + [Collaborations](https://2018.igem.org/Team%3ANUDT_CHINA/Collaborations)
* PROJECT
  + [Description](https://2018.igem.org/Team%3ANUDT_CHINA/Description)
  + [Design](https://2018.igem.org/Team%3ANUDT_CHINA/Design)
  + [Experiments](https://2018.igem.org/Team%3ANUDT_CHINA/Experiments)
  + [Notebook](https://2018.igem.org/Team%3ANUDT_CHINA/Notebook)
  + [InterLab](https://2018.igem.org/Team%3ANUDT_CHINA/InterLab)
  + [Results](https://2018.igem.org/Team%3ANUDT_CHINA/Results)
  + [Demonstrate](https://2018.igem.org/Team%3ANUDT_CHINA/Demonstrate)
  + [Improve](https://2018.igem.org/Team%3ANUDT_CHINA/Improve)
  + [Attributions](https://2018.igem.org/Team%3ANUDT_CHINA/Attributions)
* MODEL
  + [Model](https://2018.igem.org/Team%3ANUDT_CHINA/Model)
  + [Overview](https://2018.igem.org/Team%3ANUDT_CHINA/Model/Overview)
  + [Basic Design](https://2018.igem.org/Team%3ANUDT_CHINA/Model/Basic%20Design)
  + [Deduction](https://2018.igem.org/Team%3ANUDT_CHINA/Model/Deduction)
  + [Discussion](https://2018.igem.org/Team%3ANUDT_CHINA/Model/Discussion)
* PARTS
  + [Parts Overview](https://2018.igem.org/Team%3ANUDT_CHINA/Parts)
  + [Basic Parts](https://2018.igem.org/Team%3ANUDT_CHINA/Basic_Part)
  + [Composite Parts](https://2018.igem.org/Team%3ANUDT_CHINA/Composite_Part)
  + [Part Collection](https://2018.igem.org/Team%3ANUDT_CHINA/Part_Collection)
* [SAFETY](https://2018.igem.org/Team%3ANUDT_CHINA/Safety)
* Human Practices
  + [Human Practices](https://2018.igem.org/Team%3ANUDT_CHINA/Human_Practices)
  + [Education & Engagement](https://2018.igem.org/Team%3ANUDT_CHINA/Public_Engagement)
* AWARDS
  + [Model](https://2018.igem.org/Team%3ANUDT_CHINA/Model)

Designed Protein Degradation Method Based on

Trim21 And Nanobody              -- Results

## Proof of concept：

![300x200](https://static.igem.org/mediawiki/2018/8/8e/T--NUDT_CHINA--results1.jpg)

## Demostration：

![300x200](https://static.igem.org/mediawiki/2018/8/8e/T--NUDT_CHINA--results1.jpg)