iGEM 2018 — Team NEFU_China

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:NEFU China/Notebook

Notebook-2018

- * [![](https://static.igem.org/mediawiki/2018/a/a9/T--NEFU_China--_Home.png)HOME](https://2018.igem.org/Team%3ANEFU_China)
  * [![](https://static.igem.org/mediawiki/2018/1/14/T--NEFU_China--_PROJECT.png)PROJECT](https://2018.igem.org/Team%3ANEFU_China/Background)
    + [BACKGROUND](https://2018.igem.org/Team%3ANEFU_China/Background)
    + [DESCRIPTION & DESIGN](https://2018.igem.org/Team%3ANEFU_China/Description)
    + [CODE BOOK](https://2018.igem.org/Team%3ANEFU_China/Coding%20book)
  * [![](https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png)EXPERIMENTS](https://2018.igem.org/Team%3ANEFU_China/Demonstrate)
    + [LOCK & KEY](https://2018.igem.org/Team%3ANEFU_China/Lock_Key)
    + [INFORMATION DESTRUCTION](https://2018.igem.org/Team%3ANEFU_China/Suicide)
    + [Pre-RNA SPLICING](https://2018.igem.org/Team%3ANEFU_China/Splicing)
    + [DEMONSTRATE](https://2018.igem.org/Team%3ANEFU_China/Demonstrate)

    ---

    + [BASIC PARTS](https://2018.igem.org/Team%3ANEFU_China/Basic_Part)
    + [COMPOSITE PARTS](https://2018.igem.org/Team%3ANEFU_China/Composite_Part)
    + [IMPROVEMENT PARTS](https://2018.igem.org/Team%3ANEFU_China/Improve)
    + [PARTS COLLECTION](https://2018.igem.org/Team%3ANEFU_China/Part_Collection)

    ---

    + [NOTEBOOK](https://2018.igem.org/Team%3ANEFU_China/Notebook)
    + [PROTOCOL](https://2018.igem.org/Team%3ANEFU_China/Protocol)
  * [![](https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png)MODEL](https://2018.igem.org/Team%3ANEFU_China/Model)
    + [OVERVIEW](https://2018.igem.org/Team%3ANEFU_China/Model)
    + [CORRESPONDING COEFFICIENT](https://2018.igem.org/Team%3ANEFU_China/Model1)
    + [KILLING MODEL](https://2018.igem.org/Team%3ANEFU_China/Model2)
  * [![](https://static.igem.org/mediawiki/2018/c/c0/T--NEFU_China--_SOFTWARE.png)SOFTWARE](https://2018.igem.org/Team%3ANEFU_China/Software)
    + [OVERVIEW](https://2018.igem.org/Team%3ANEFU_China/Software)
    + [CODING](https://2018.igem.org/Team%3ANEFU_China/Software1)
    + [MISLEADING](https://2018.igem.org/Team%3ANEFU_China/Software2)
    + [WORDSEGMENT](https://2018.igem.org/Team%3ANEFU_China/Software3)
  * [![](https://static.igem.org/mediawiki/2018/5/50/T--NEFU_China--_TEAM.png)TEAM](https://2018.igem.org/Team%3ANEFU_China/Attributions)
    + [ATTRIBUTIONS](https://2018.igem.org/Team%3ANEFU_China/Attributions)
    + [MEMBERS](https://2018.igem.org/Team%3ANEFU_China/Members)
  * [![](https://static.igem.org/mediawiki/2018/9/91/T--NEFU_China--_HUMANPRACTICE.png)HUMAN PRACTICE](https://2018.igem.org/Team%3ANEFU_China/Human_Practices)
    + [OVERVIEW](https://2018.igem.org/Team%3ANEFU_China/Human_Practices)
    + [GOLD INTEGRATED](https://2018.igem.org/Team%3ANEFU_China/Gold_integrated)
    + [SILVER](https://2018.igem.org/Team%3ANEFU_China/Silver)
    + [COLLABORTION](https://2018.igem.org/Team%3ANEFU_China/Collaborations)
    + [EDUCATION & PUBLIC ENGAGEMENT](https://2018.igem.org/Team%3ANEFU_China/Public_Engagement)

![Protocol](https://static.igem.org/mediawiki/2018/0/02/T--NEFU_China--Notebook.png)

# 2018.04.27

# Come up with ideas about multi-level bio-encryption systems and search for information to improve scheme design.

# 2018.05.09

# Design a synthetic coding sequence for the stem-loop structure as the lock part of our scheme.

# 2018.05.12

# Design synthetic coding sequence of antisense RNA which can be matched to our designed stem-loop.

# 2018.05.28

# Intercepting the homologous arm sequence from the transposons of the yeast that can integrate the encrypted information into the yeast and synthesizing it.

# 2018.08.24

# Convert our encrypted information to the corresponding DNA sequence according to our encryption principle, and design its primers.

# 2018.09.15

# Verify the expression of the downstream gene of the Fig2C promoter induced by the α factor.

# 2018.09.20

# Verify the functionality of the stem loop as the lock part of our scheme.

# 2018.09.25

# Interaction between the lock and the key, the correct information can be expressed after adding the key.

# 2018.09.30

# Verify the expression level of the improved part pFUS1.

# 2018.10.10

# The promoter function in the information destruction system is confirmed.

# 2018.10.15

# Verify that the correct information can be expressed after adding the key.

# 2018.10.17

# Finish the wiki.


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:NEFU China/Experiments

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[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3ANEFU_China)
[HOME](https://2018.igem.org/Team%3ANEFU_China)

TEAM

[Team Members](https://2018.igem.org/Team%3ANEFU_China/Team)
[Collaborations](https://2018.igem.org/Team%3ANEFU_China/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ANEFU_China/Description)
[Design](https://2018.igem.org/Team%3ANEFU_China/Design)
[Experiments](https://2018.igem.org/Team%3ANEFU_China/Experiments)
[Notebook](https://2018.igem.org/Team%3ANEFU_China/Notebook)
[InterLab](https://2018.igem.org/Team%3ANEFU_China/InterLab)
[Model](https://2018.igem.org/Team%3ANEFU_China/Model)
[Results](https://2018.igem.org/Team%3ANEFU_China/Results)
[Demonstrate](https://2018.igem.org/Team%3ANEFU_China/Demonstrate)
[Improve](https://2018.igem.org/Team%3ANEFU_China/Improve)
[Attributions](https://2018.igem.org/Team%3ANEFU_China/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ANEFU_China/Parts)
[Basic Parts](https://2018.igem.org/Team%3ANEFU_China/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ANEFU_China/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ANEFU_China/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ANEFU_China/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ANEFU_China/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ANEFU_China/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ANEFU_China/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ANEFU_China/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ANEFU_China/Hardware)
[Measurement](https://2018.igem.org/Team%3ANEFU_China/Measurement)
[Model](https://2018.igem.org/Team%3ANEFU_China/Model)
[Plant](https://2018.igem.org/Team%3ANEFU_China/Plant)
[Software](https://2018.igem.org/Team%3ANEFU_China/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=NEFU_China)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:NEFU China/Results

![](https://static.igem.org/mediawiki/2018/4/4f/2018_igem_mobile_menu_logo.svg)

![](https://static.igem.org/mediawiki/igem.org/3/3b/Menu_icon.svg)

[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3ANEFU_China)
[HOME](https://2018.igem.org/Team%3ANEFU_China)

TEAM

[Team Members](https://2018.igem.org/Team%3ANEFU_China/Team)
[Collaborations](https://2018.igem.org/Team%3ANEFU_China/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ANEFU_China/Description)
[Design](https://2018.igem.org/Team%3ANEFU_China/Design)
[Experiments](https://2018.igem.org/Team%3ANEFU_China/Experiments)
[Notebook](https://2018.igem.org/Team%3ANEFU_China/Notebook)
[InterLab](https://2018.igem.org/Team%3ANEFU_China/InterLab)
[Model](https://2018.igem.org/Team%3ANEFU_China/Model)
[Results](https://2018.igem.org/Team%3ANEFU_China/Results)
[Demonstrate](https://2018.igem.org/Team%3ANEFU_China/Demonstrate)
[Improve](https://2018.igem.org/Team%3ANEFU_China/Improve)
[Attributions](https://2018.igem.org/Team%3ANEFU_China/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ANEFU_China/Parts)
[Basic Parts](https://2018.igem.org/Team%3ANEFU_China/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ANEFU_China/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ANEFU_China/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ANEFU_China/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ANEFU_China/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ANEFU_China/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ANEFU_China/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ANEFU_China/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ANEFU_China/Hardware)
[Measurement](https://2018.igem.org/Team%3ANEFU_China/Measurement)
[Model](https://2018.igem.org/Team%3ANEFU_China/Model)
[Plant](https://2018.igem.org/Team%3ANEFU_China/Plant)
[Software](https://2018.igem.org/Team%3ANEFU_China/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=NEFU_China)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)