iGEM 2016 — Team Alverno_CA

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# Team:Alverno CA/Notebook

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* Project
  + [Description](https://2016.igem.org/Team%3AAlverno_CA/Description)
  + [Design](https://2016.igem.org/Team%3AAlverno_CA/Design)
  + [Experiment](https://2016.igem.org/Team%3AAlverno_CA/Experiment)
  + [Protocols](https://2016.igem.org/Team%3AAlverno_CA/Protocols)
  + [Engagement](https://2016.igem.org/Team%3AAlverno_CA/Engagement)
  + [Interlab Study](https://2016.igem.org/Team%3AAlverno_CA/InterLab)
  + [Results](https://2016.igem.org/Team%3AAlverno_CA/Results)
  + [Notebook](https://2016.igem.org/Team%3AAlverno_CA/Notebook)
* [Parts](https://2016.igem.org/Team%3AAlverno_CA/Basic_Parts)
* [Safety](https://2016.igem.org/Team%3AAlverno_CA/Safety)
* [Attributions](https://2016.igem.org/Team%3AAlverno_CA/Attributions)
* [Human
  Practices](https://2016.igem.org/Team%3AAlverno_CA/Human_Practices)

Alverno iGEM 2016

## Notebook

![](https://static.igem.org/mediawiki/2016/a/ac/T--Alverno_CA--labnotbook.jpg)

### Week of June 6th (Limited amount of notes taken)

##### - Lots of Designing Parts and Primers

##### - Wiki Work

### Week of June 13th:

##### - Cleaning Restriction Digest and Ligation Done

##### - Ampicillin plates made

##### - Ampicillin stock made

##### - Transform RFP plasmid

##### - Design Parts and Primers

##### - Wiki Work

### Week of June 20th

##### - Transformation Done (probably GFP)

##### - RFP and GRP colonies

##### - PCR for RFP Coding Gene

##### - Design Parts and Primers

##### - Wiki Work

### Week of June 27th

##### - PCR Done for promoters, terminators, some UC parts

##### - Primer Dilutions Done

##### - Gel Run with PCR product from Week of June 20th (RFP)

##### - Miniprep kit for RFP Bba

##### - TBE Buffer 10x and 1x made

##### - PCR Purification done on Promoters and Terminators

##### - Wiki Work

### Week of July 4th

##### - PCR of Vectors, some UC parts

##### - Primer Dilutions

##### - Gel Run UC16-18 and UC2 (UC18 ran twice)

##### - PCR Clean Up done for UC16-18, vectors, T3-T4 (terminators), UC2 (UC18 done twice)

##### - Plate Reader

##### - Wiki Work

### Week of July 11th

![iGEM](https://static.igem.org/mediawiki/2016/4/44/T--Alverno_CA--Screenshot_2016-10-15_09.24.58.png)

##### - Learn how to use Benchling

##### - Primers Diluted

![iGEM](https://static.igem.org/mediawiki/2016/a/ad/T--Alverno_CA--Screenshot_2016-10-15_09.05.png)

##### - Parts Diluted

##### - PCR done on P1ab, P2ab, T3ab, T4ab

##### - Gel Run on P1ab, P2ab, T3ab, T4ab

##### - PCR Clean up done on P1ab, P2ab, T3ab, T4ab

##### - Chloramphenicol plates made

##### - Wiki Work

### Week of July 18th

##### - UCLA iGEM meetup (UCLA, ULV, UCSD, and Marburg Teams)

![iGEM](https://static.igem.org/mediawiki/2016/d/d5/T--Alverno_CA--Screenshot_2016-10-15_09.05.53.png)

##### - Golden Gate Assembly Done with P1ab, P2ab, P3, P4, T1, T2, T3ab, T4ab, UC2, V2d

![iGEM](https://static.igem.org/mediawiki/2016/5/5d/T--Alverno_CA--Screenshot_2016-10-15_09.06.03.png)

##### - Transformation Done

##### - Chloramphenicol plates made

##### - Parts dilutions Done

##### - PCR of GG Assemblies

##### - PCR of UC3a-UC9a

##### - Wiki Work

### Week of July 25th

![iGEM](https://static.igem.org/mediawiki/2016/4/4f/T--Alverno_CA--Screenshot_2016-10-15_09.06.12.png)

##### - Set up Plate reader

##### - Run Gel UC3a-UC9a & GG1-8, V1a & V2a, GG Parts

##### - PCR Purification of UC3-9, V1a & V2a

![iGEM](https://static.igem.org/mediawiki/2016/d/d9/T--Alverno_CA--Screenshot_2016-10-15_09.06.21.png)

##### - Picked Colonies

##### - Transformation Protocol for GG1-8

##### - Gel made & Gel made w/ SYBR safe

##### - PCR of GG Assemblies

##### - PCR for V1a and V2a, UC7-9

##### - Dilutions Done

##### - Gel Run for UC7-9

##### - Golden Gate Assembly GG9-16

##### - Wiki Work

### Week of August 1st

##### - Run Gel for UC7-9 & (GG9-16 Done twice) & GG17-19 & GG20-23

##### - PCR of GFP & RFP w/ varying UTRs & RFP chlor. GFP chlor. Kan GG12

##### - PCR Purification for UC7-8

##### - Parts Dilutions done UC2 & UC3-6 & UC7-8

##### - PCR Check for GG9-16 (Done twice) & GG17-19 & GG20-23

![iGEM](https://static.igem.org/mediawiki/2016/d/d6/T--Alverno_CA--Screenshot_2016-10-15_09.06.31.png)

##### - Fixed Thermocycler

##### - Gels Made

##### - Agar Plates made w/ Kan

##### - Golden Gate Assembly GG17-19 & GG20-23 & GG24-26

![iGEM](https://static.igem.org/mediawiki/2016/c/c2/T--Alverno_CA--Screenshot_2016-10-15_09.06.45.png)

##### - Transformation with GG12

##### - Ludox Calibration

##### - Test Fluorescence of Plasmids

##### - Wiki Work

### Week of August 8th

![iGEM](https://static.igem.org/mediawiki/2016/5/53/T--Alverno_CA--Screenshot_2016-10-15_09.06.52.png)

##### - Cell Measurement

![iGEM](https://static.igem.org/mediawiki/2016/0/0e/T--Alverno_CA--Screenshot_2016-10-15_09.07.03.png)

##### - Run Gel for GG24-26 & 24A-E, 25A-E, 26A-E, 27-28 PCR

##### - PCR Check for GG24-26 & GG27-28

##### - Made Gel

##### - Transformation with GG24-26 & GG27-28

##### - Golden Gate Assembly GG27-28

##### - Colony PCR Check of GG24-26

##### - Primer Dilutions

##### - Plasmid DNA Purification

##### - LB Kan Broth

##### - Wiki Work

### Week of August 15th

![iGEM](https://static.igem.org/mediawiki/2016/5/5c/T--Alverno_CA--Screenshot_2016-10-15_09.07.17.png)

##### - Lab Meet-up with UCL/LC/CV

##### - Colony PCR Check for GG libraries 27-28

##### - Gels Made

##### - Gel Run for GG Library 28

##### - Grow Cultures GG27

![iGEM](https://static.igem.org/mediawiki/2016/0/0c/T--Alverno_CA--Screenshot_2016-10-15_09.07.26.png)

##### - Learn Python

##### - Check Fluorescence for GG28 & GG27

##### - PCR UC14a

##### - Purification of Plasmid DNA

##### - Wiki Work

### Week of August 22nd

![iGEM](https://static.igem.org/mediawiki/2016/a/ad/T--Alverno_CA--Screenshot_2016-10-15_09.07.34.png)

##### - Gel Run for GG27 & UC13-15 UC13-15 and UC1 & GG29-36

##### - Gels Made

##### - PCR UC13a and UC15a (Done twice) & UC1

##### - PCR Purification of UC14 & UC13/15 & UC1

##### - Golden Gate Assembly for GG29-36

##### - PCR Check for GG29-36

##### - LB Kan Broth Made

##### - Dilution of UC1

##### - Control run in Plate Reader

![iGEM](https://static.igem.org/mediawiki/2016/6/69/T--Alverno_CA--Screenshot_2016-10-15_09.07.46.png)

##### - Wiki Work

### Week of August 29th

![iGEM](https://static.igem.org/mediawiki/2016/9/9b/T--Alverno_CA--Screenshot_2016-10-15_09.07.54.png)

##### - Make Gels

##### - Run Gel for UC13-UC15 & GG29-36 and CDS1a & CDS2a

##### - Golden Gate Assembly GG37-52 & GG53-60

##### - PCR Check GG37-52 & GG53-60

##### - PCR Check Gel run for GG37-52

##### - PCR CDS1a & CDS2a

##### - PCR Purification of CDS1a & CDS2a

![iGEM](https://static.igem.org/mediawiki/2016/d/d7/T--Alverno_CA--Screenshot_2016-10-15_09.08.02.png)

##### - Talk with iGEM Peshawar

##### - LB Kan Plates Made

##### - Wiki Work

### Week of September 5th

![iGEM](https://static.igem.org/mediawiki/2016/a/ae/T--Alverno_CA--Screenshot_2016-10-15_09.08.11.png)

##### - iGEM Team Meeting

##### - PCR Purification of CDS1a & CDS2a and UC1

##### - Transformation was done with GG53-60

##### - Gel Run for CDS1 & CDS2 and UC1 (done 3 times) and GG61-64

##### - Gels made

##### - Dilution of CDS1a & CDS2a

##### - Golden Gate Assembly GG61-64

##### - PCR of UC1

##### - LB Kan Plates Made

##### - PCR Check for GG61-64

![iGEM](https://static.igem.org/mediawiki/2016/c/c1/T--Alverno_CA--Screenshot_2016-10-15_09.08.20.png)

##### - TBE 10x Buffer

##### - Wiki Work

### Week of September 12th

![iGEM](https://static.igem.org/mediawiki/2016/f/f2/T--Alverno_CA--Screenshot_2016-10-15_09.08.27.png)

##### - Talked with UCL iGEM Team

##### - LV-LC-CV meet-up

##### - Google Hangouts with Lambert Georgia iGEM team

![iGEM](https://static.igem.org/mediawiki/2016/c/c1/T--Alverno_CA--Screenshot_2016-10-15_09.08.35.png)

##### - Dilution of UC1a

##### - Transformation of GG29-36

##### - Golden Gate Assembly GG65-72

##### - PCR GG61-64

##### - PCR Check GG61-64

##### - TX-TL of GFP (+ control)

![iGEM](https://static.igem.org/mediawiki/2016/0/04/T--Alverno_CA--Screenshot_2016-10-15_09.08.42.png)

##### - Outreach workshop with Middle school

##### - Wiki Work

### Week of September 19th

##### - REVIEW OF EVERYTHING THAT NEEDS TO BE DONE BEFORE GOING TO JAMBOREE

![iGEM](https://static.igem.org/mediawiki/2016/4/4a/T--Alverno_CA--Screenshot_2016-10-15_09.08.50.png)

##### - Run Gel for GG61-64 (done twice) and GG65-72

##### - LB Kan Plates Made

##### - Gels Made

##### - TX-TL GFP Re-run

##### - Gel Run for UC1 and GG61-64

##### - Control Test ran using only the ladder for Gels

##### - TX-TL W & M plasmids

##### - PCR U3a

##### - Dilution of Primers

![iGEM](https://static.igem.org/mediawiki/2016/3/3f/T--Alverno_CA--Screenshot_2016-10-15_09.09.01.png)

##### - Wiki Work

### Week of September 26th

##### - PCR Purification of V3a

##### - Golden Gate Assembly GG73-74 and GG75-82 and GG83-90 and GG91-94

##### - PCR Check GG73-74 and GG75-82

##### - Dilution V3a

##### - Run Gel V3a

##### - Transformation of GG61-64 (done twice) and GG65-72 and GG73-74

##### - TX-TL re-run for W & M plasmids

##### - Colony PCR GG61-64 and GG65-72

![iGEM](https://static.igem.org/mediawiki/2016/c/c6/T--Alverno_CA--Screenshot_2016-10-15_09.09.08.png)

##### - LB Chloramphenicol plates & LB Kanamycin plates

![iGEM](https://static.igem.org/mediawiki/2016/6/65/T--Alverno_CA--Screenshot_2016-10-15_09.09.17.png)

##### - Wiki Work

### Week of October 3rd

![iGEM](https://static.igem.org/mediawiki/2016/5/50/T--Alverno_CA--Screenshot_2016-10-15_09.09.24.png)

##### - Middle School Visit

##### - LB Broth made

##### - PCR P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab

##### - Dilution V3a

##### - PCR Check GG83-90

##### - PCR Purification P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab

##### - TX-TL re-run for W & M plasmids w/ Replicates

##### - Gels Made

##### - Mini prep GG28 1-5

##### - Make TBE 1x

![iGEM](https://static.igem.org/mediawiki/2016/9/99/T--Alverno_CA--Screenshot_2016-10-15_09.09.30.png)

##### - Gel Run P1a-P4a, T1a-T4a, P1ab, P2ab, T3ab, T4ab & GG95-102

##### - Primers Diluted

##### - Golden Gate GG103 & GG104 & GG85-102 & GG105-112

##### - Transformation GG95-102 (done twice)

##### - Testing Fluorescence on GG28 1-5 w/ Plate Reader

##### - Wiki Work

### Week of October 10th

##### - Golden Gate GG113-114

##### - Transformation GG103 & 104, GG105-112

##### - LB Chloramphenicol plates made

##### - PCR Purification of UC13a-UC15a

##### - Transformation done GG113-114

##### - LB Broth Made

##### - Mini Prep GG95 (1)-GG102 (3)

##### - Dilute Primers

##### - Test Fluorescence of GG95-102\_1-3 & GG105-108\_1-4 (by TX-TL)

##### - Purification of Plasmid DNA GG95-102\_1-3 & GG105-108\_1-4

### Week of October 17th

##### - WIKI WORK!!!

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