iGEM 2016 — Team Groningen

[![](https://static.igem.org/mediawiki/2016/8/8b/HQ_page_logo.jpg)](https://2016.igem.org)

# Team:Groningen/Notebook

﻿

[![CryptoGE®M](https://static.igem.org/mediawiki/2016/5/5d/T--Groningen--logo-200.png)](/Team%3AGroningen)

[CryptoGE®M](/Team%3AGroningen)

![Team](https://static.igem.org/mediawiki/2016/f/fc/T--Groningen--menu-toolbox.png)

Team

[Members](/Team%3AGroningen/Team)
[Supervisors](/Team%3AGroningen/Supervisors)
[Collaborations](/Team%3AGroningen/Collaborations)
[Achievements](/Team%3AGroningen/Achievements)
[Contact](/Team%3AGroningen/Contact)

![Project](https://static.igem.org/mediawiki/2016/2/2b/T--Groningen--menu-idea.png)

Project

[**Tour**](/Team%3AGroningen/Tour)
[Description](/Team%3AGroningen/Description)
[Design](/Team%3AGroningen/Design)
[Results](/Team%3AGroningen/Results)
[Demonstrate](/Team%3AGroningen/Demonstrate)
[Applications](/Team%3AGroningen/Applications)
[Future](/Team%3AGroningen/FuturePerspectives)
[Notebook](/Team%3AGroningen/Notebook)

![Biology](https://static.igem.org/mediawiki/2016/8/8b/T--Groningen--menu-streak.png)

Biology

[Experiments](/Team%3AGroningen/Experiments)
[Parts](/Team%3AGroningen/Parts)
[Safety](/Team%3AGroningen/Safety)
[Lab journal](/Team%3AGroningen/Labjournal)

![Computing](https://static.igem.org/mediawiki/2016/1/18/T--Groningen--menu-software.png)

Computing

[Modelling](/Team%3AGroningen/Model)
[Software](/Team%3AGroningen/Software)
[Encoding](/Team%3AGroningen/Encoding)
[Decoding](/Team%3AGroningen/Decoding)

![Human Practice](https://static.igem.org/mediawiki/2016/5/54/T--Groningen--menu-teamwork.png)

Human Practices

[Human Practices](/Team%3AGroningen/Human_Practices)
[Gold](/Team%3AGroningen/HP/Gold)
[Silver](/Team%3AGroningen/HP/Silver)

![Acknowledgements](https://static.igem.org/mediawiki/2016/2/26/T--Groningen--menu-modelling.png)

Support

[Sponsors](/Team%3AGroningen/Sponsors)
[Attributions](/Team%3AGroningen/Attributions)

---

## Notebook

#### Week 1: Jan 10 – Jan 16

|  |  |
| --- | --- |
| General | team forming (14.1.2016) |

![](https://static.igem.org/mediawiki/2016/thumb/0/0e/T--Groningen--Notebook-Notification-First-Meeting.jpg/730px-T--Groningen--Notebook-Notification-First-Meeting.jpg)

#### Week 2: Jan 17 – Jan 23

|  |  |
| --- | --- |
| General | write motivation letter |

#### Week 4: Jan 31 – Feb 6

|  |  |
| --- | --- |
| General | weekly meeting: team forming |

![](https://static.igem.org/mediawiki/2016/b/b9/T--Groningen--team-team.jpg)

#### Week 5: Feb 7 – Feb 13

|  |  |
| --- | --- |
| General | weekly meeting: team forming |

#### Week 6: Feb 14 – Feb 20

|  |  |
| --- | --- |
| General | weekly meeting: team forming |

![](https://static.igem.org/mediawiki/2016/thumb/5/51/T--Groningen--week6.jpg/800px-T--Groningen--week6.jpg)

#### Week 8: Feb 28 – Mar 5

|  |  |
| --- | --- |
| General | weekly meeting: project brainstorming |

#### Week 9: Mar 6 – Mar 12

|  |  |
| --- | --- |
| General | weekly meeting: project brainstorming |

#### Week 10: Mar 13 – Mar 19

|  |  |
| --- | --- |
| General | weekly meeting: project brainstorming |

#### Week 11: Mar 20 – Mar 26

|  |  |
| --- | --- |
| General | weekly meeting: project brainstorming |

![](https://static.igem.org/mediawiki/2016/thumb/5/5d/T--Groningen--Notebook-MeetingiGEM2016.jpg/800px-T--Groningen--Notebook-MeetingiGEM2016.jpg)

#### Week 12: Mar 27 – Apr 2

|  |  |
| --- | --- |
| General | weekly meeting: decide on project |

![](https://static.igem.org/mediawiki/2016/thumb/e/ee/T--Groningen--CriminalDude.png/489px-T--Groningen--CriminalDude.png)

#### Week 13: Apr 3 – Apr 9

|  |  |
| --- | --- |
| HP | Preparations for NBC |

![](https://static.igem.org/mediawiki/2016/thumb/9/92/T--Groningen--week13.jpg/800px-T--Groningen--week13.jpg)

#### Week 14: Apr 10 – Apr 16

|  |  |
| --- | --- |
| HP | NBC Wageningen |
| Coding/Wiki | Start with DNA encoder |

![](https://static.igem.org/mediawiki/2016/thumb/4/45/T--Groningen--First-Presentation-at-NBC-0Wageningen.jpg/800px-T--Groningen--First-Presentation-at-NBC-0Wageningen.jpg)

#### Week 15: Apr 17 – Apr 23

|  |  |
| --- | --- |
| Coding/Wiki | DNA encoder |

![](https://static.igem.org/mediawiki/2016/9/97/T--Groningen--week15.jpg)

#### Week 16: Apr 24 – Apr 30

|  |  |
| --- | --- |
| Coding/Wiki | First version of encoder done |
| General lab | starting of setting up the lab |

![](https://static.igem.org/mediawiki/2016/thumb/5/56/T--Groningen--SetupLab.jpg/800px-T--Groningen--SetupLab.jpg)

#### Week 17: May 1 – May 7

|  |  |
| --- | --- |
| General lab | setting up the lab |

![](https://static.igem.org/mediawiki/2016/thumb/2/28/T--Groningen--week17.jpg/800px-T--Groningen--week17.jpg)

#### Week 18: May 8 – May 14

|  |  |
| --- | --- |
| HP | Preparation for Meeting of Dutch Teams Wageningen |
| Modelling/Coding | Start with MultiAgent System Model |
| Coding/Wiki | DNA encoder |
| General lab | setting up the lab |
| Integration/Lab | plan integration cloning |

![](https://static.igem.org/mediawiki/2016/thumb/0/0c/T--Groningen--week18.jpg/800px-T--Groningen--week18.jpg)

#### Week 19: May 15 – May 21

|  |  |
| --- | --- |
| HP | Meeting of Dutch Teams - Wageningen |
| Modelling/Coding | Literature MAS |
| Coding/Wiki | DNA encoder |
| General lab | setting up the lab, free license from eLab journal received |
| Integration/Lab | plan integration cloning |

![](https://static.igem.org/mediawiki/2016/thumb/e/e9/T--Wageningen_UR--National2.jpg/800px-T--Wageningen_UR--National2.jpg)

#### Week 20: May 22 – May 28

|  |  |
| --- | --- |
| HP | Campus Party Attendance |
| Modelling/Coding | Literature MAS |
| General lab | setting up the lab |
| Integration/Lab | plan integration cloning |

![](https://static.igem.org/mediawiki/2016/thumb/2/2e/T--Groningen--week20.jpeg/800px-T--Groningen--week20.jpeg)

#### Week 21: May 29 – Jun 4

|  |  |
| --- | --- |
| HP | Night of Art and Sciences Participation (ScienceLinx) |
| Modelling/Coding | Literature MAS |
| Integration/Lab | plan integration cloning |

![](https://static.igem.org/mediawiki/2016/thumb/2/2d/T--Groningen--week21.jpg/799px-T--Groningen--week21.jpg)

#### Week 22: Jun 5 – Jun 11

|  |  |
| --- | --- |
| Modelling/Coding |  |
| Integration/Lab | plan integration cloning, prepare medium for *Bacillus subtilis* transformation, |
| Antibiotics/Lab | Preparing sporulation medium |
| RIVM - Safe by Design | First Phone call with RIVM |

![](https://static.igem.org/mediawiki/2016/2/24/T--Groningen--Notebook-prep-lb.jpg)

#### Week 23: Jun 12 – Jun 18

|  |  |
| --- | --- |
| Modelling/Coding | Start MAS Coding |
| General lab | 16.6. first order of lab needs |
| Integration/Lab | plan integration cloning |
| Antibiotics/Lab | Sporulation of *B. subtilis* 168 |
| RIVM - Safe by Design | Working on proposal Safe by Design |

![](https://static.igem.org/mediawiki/2016/thumb/1/1a/T--Groningen--week23.jpg/800px-T--Groningen--week23.jpg)

#### Week 24: Jun 19 – Jun 25

|  |  |
| --- | --- |
| Modelling/Coding | Coding |
| Coding/Wiki | Start with wiki |
| Integration/Lab | plan integration cloning |
| Antibiotics/Lab | Testing UV resistance of spores |
| Collaboration Wageningen | skype with Wageningen |
| RIVM - Safe by Design | Working on proposal Safe by Design |

![](https://static.igem.org/mediawiki/2016/8/84/T--Groningen--Notebook-Working-On-The-Wiki-Marco.jpg)

#### Week 25: Jun 26 – Jul 2

|  |  |
| --- | --- |
| HP | Research National regulations for Sending |
| Modelling/Coding | Coding |
| Integration/Lab | plan integration cloning |
| RIVM - Safe by Design | Working on proposal Safe by Design |

![](https://static.igem.org/mediawiki/2016/thumb/3/3b/T--Groningen--week25.jpg/800px-T--Groningen--week25.jpg)

#### Week 26: Jul 3 – Jul 9

|  |  |
| --- | --- |
| HP | Research National Regulations for Sending |
| Modelling/Coding | Genetic Algorithm finished |
| Coding/Wiki | DNA encoder |
| Integration/Lab | plan integration cloning |
| Antibiotics/Lab | Determining optimal plating dilution, MIC of ciprofloxacin |
| RIVM - Safe by Design | First Meeting with RIVM |

![](https://static.igem.org/mediawiki/2016/thumb/d/d1/T--Groningen--Notebook-1stMeeting-RIVM.jpg/800px-T--Groningen--Notebook-1stMeeting-RIVM.jpg)

#### Week 27: Jul 10 – Jul 16

|  |  |
| --- | --- |
| HP | Contact National Cyber Security Center and RIVM for orientation in regulations |
| Modelling/Coding | Coding |
| Coding/Wiki | DNA format finalized |
| General lab | 13.7. first order of the gBlocks |
| Integration/Lab | message and key gBlock order |
| Antibiotics/Lab | Ciprofloxacin MIC and MBC, patterning experiment |
| RIVM - Safe by Design | Working on product of Proposal (booklet) Safe by Design |

![](https://static.igem.org/mediawiki/2016/thumb/5/54/T--Groningen--week27.jpg/800px-T--Groningen--week27.jpg)

#### Week 28: Jul 17 – Jul 23

|  |  |
| --- | --- |
| HP | Research International Regulations for Sending/Planning Future Scenearios/Collaboration with iGEM Teams for International Survey |
| Modelling/Coding | Coding |
| General lab | 19.7.clone manager tutorial by Bara, 22.7. half of the gBlocks received, 21.7. competent cells preparation, digestion of linearized plasmid backbones |
| Integration/Lab | message gBlock received -> start of the cloning process |
| Kill switch/Lab | E.coli top10 competent cell preparation, basic layout of kill switches |
| Antibiotics/Lab | Spirofloxacin MIC tests and Photoswitch test |
| RIVM - Safe by Design | Working on product of Proposal (booklet) Safe by Design |

![](https://static.igem.org/mediawiki/2016/thumb/5/50/T--Groningen--week28.jpg/800px-T--Groningen--week28.jpg)

#### Week 29: Jul 24 – Jul 30

|  |  |
| --- | --- |
| HP | Release of the Survey to social Media/Planning Future Scnearios |
| Modelling/Coding | First Results Sender |
| General lab | second half of the gBlocks received, preparation of agarose for the gels, amplifying of gBlocks |
| Integration/Lab | cloning procedure of the message into pdr111 (E.coli TOP10) |
| Kill switch/Lab | Primer design, Detailed kill switch layout; nuclease biobrick order |
| Antibiotics/Lab | Spirofloxacin MIC tests |
| Spores/Lab | Test Tonia's and Daniel's protocols |
| RIVM - Safe by Design | Working on product of Proposal (booklet) Safe by Design |

![](https://static.igem.org/mediawiki/2016/thumb/d/d4/T--Groningen--Notebook-Gblocks.jpeg/450px-T--Groningen--Notebook-Gblocks.jpeg)

#### Week 30: Jul 31 – Aug 6

|  |  |
| --- | --- |
| HP | Interview JanJaap and visit to Groninger Archieven/RADIO PARTICIPATION |
| Modelling/Coding | First Results Criminal |
| Integration/Lab | pDR111+message transformation to *Bacillus subtilis* trp+, starch test to verify right colonies, samples for sequencing |
| Kill switch/Lab | Cloning - RBS (34), tetR and 2terminator; Cloning repeat - RBS (34) |
| Antibiotics/Lab | Spirofloxacin MIC tests with E. coli |
| Spores/Lab | Test Tonia's and Daniel's protocols |
| RIVM - Safe by Design | Working on product of Proposal (booklet) Safe by Design |

![](https://static.igem.org/mediawiki/2016/e/e2/T--Groningen--Notebook-sambit.gif)

#### Week 31: Aug 7 – Aug 13

|  |  |
| --- | --- |
| HP | Planning Future Scenarios/Working on presentation for ScienceLinX Day |
| Modelling/Coding | MAS Paper + Wiki |
| General lab | amplification of gBlocks |
| Integration/Lab | cloning procedure of pDR111+message+sfGFP and pdr111+sfGFP |
| Kill switch/Lab | Cloning - RBS (34) |
| Antibiotics/Lab | Spirofloxacin MIC tests with E. coli |
| Spores/Lab | Daniel's protocol worked best and fastest |
| Collaboration Wageningen | vitamin b12 and guanine stock, recive biobrick |
| RIVM - Safe by Design | Second Phone Call RIVM |
| CRISPR/CAS9 | Starting a new idea (using CRISPR/Cas 9) |
| decoy/sfGFP | cloning plan sfGFP |

![](https://static.igem.org/mediawiki/2016/thumb/8/85/T--Groningen--week35.jpg/800px-T--Groningen--week35.jpg)

#### Week 32: Aug 14 – Aug 20

|  |  |
| --- | --- |
| HP | Elaboration of Proposal of Labelling of GMOs |
| Modelling/Coding | Start of Random Mutation Model |
| Integration/Lab | problems with gBlock |
| Kill switch/Lab | Genomic DNA for nuclease; pimer design for nuclease |
| Antibiotics/Lab | Ciprofloxacin-resistant B. sub. by directed evolution |
| Collaboration Wageningen | plan plate reader experiment |
| RIVM - Safe by Design | Working on product of Proposal (booklet) Safe by Design |
| CRISPR/CAS9 | Worked on design plan (e.g. primers, plasmids, labwork) |
| decoy/sfGFP | cloning sfGFP in pDR111/ sequencing validation |

![](https://static.igem.org/mediawiki/2016/thumb/2/21/T--Groningen--Notebook-plate-reader.jpg/800px-T--Groningen--Notebook-plate-reader.jpg)

#### Week 33: Aug 21 – Aug 27

|  |  |
| --- | --- |
| HP | Planning Future Scenarios/Working on presentation for ScienceLinX Day/Prepairing Lab equipment etc for SienceLinX Day |
| Modelling/Coding | Literature Model 2 |
| Coding/Wiki | Wiki |
| General lab | 22.8. second order of the gBlocks |
| Integration/Lab | problems with gBlock, ordering of the new key sequence as a gBlock |
| Kill switch/Lab | Cloning - RBS (30); nuclease biobrick recieved |
| Antibiotics/Lab | Ciprofloxacin-resistant B. sub. by directed evolution |
| Collaboration Wageningen | microscopy, repeat plate reader experiment |
| RIVM - Safe by Design | Working on product of Proposal (booklet) Safe by Design |
| CRISPR/CAS9 | Worked on design plan (e.g. primers, plasmids, labwork) |
| decoy/sfGFP | integration of sfGFP pDR111 into the genome of *B. subtilis* |

![](https://static.igem.org/mediawiki/2016/1/16/T--Groningen--week33.jpeg)

#### Week 34: Aug 28 – Sep 3

|  |  |
| --- | --- |
| HP | ScienceLinX Day/BioART with students/GBB Meeting |
| Modelling/Coding | Coding + Results |
| Coding/Wiki | Wiki |
| General lab | 2.9. second order of lab needs, gBlock received |
| Integration/Lab | key gBlock received -> start of the cloning process, cloning procedure of the key into pdr111 (E.coli TOP10) |
| Kill switch/Lab | Cloning - RBS (30) + nuclease, tetR + RBS (30) + nuclease |
| Antibiotics/Lab | Ciprofloxacin-resistant B. sub. by directed evolution |
| Collaboration Wageningen | repeat plate reader experiment |
| RIVM - Safe by Design | Third Phone Call RIVM |
| CRISPR/CAS9 | Worked on design plan (e.g. primers, plasmids, labwork) |
| decoy/sfGFP | microscopy of *B.subtilis*+sfGFP |

![](https://static.igem.org/mediawiki/2016/a/a4/T--Groningen--Notebook-gbb-1.jpg)

#### Week 35: Sep 4 – Sep 10

|  |  |
| --- | --- |
| HP | Future Scenarios were completed/Meeting with Simone from the university paper |
| Modelling/Coding | More Literature + Autocorrecting Codes |
| Coding/Wiki | Wiki |
| Integration/Lab | transform pDR111+key to *Bacillus subtilis*, sequence the message obtained from spores, send spores to Wageningen and Eindhoven, time-lapse movie (germination of spores) |
| Kill switch/Lab | Cloning - tetR + RBS (30) + nuclease, tetR + RBS (30) + nuclease + 2terminator |
| Antibiotics/Lab | Ciprofloxacin-resistant B. sub. by directed evolution (experiment 1) |
| RIVM - Safe by Design | Working on product of Proposal (booklet) Safe by Design |
| CRISPR/CAS9 | Worked on design plan (e.g. primers, plasmids, labwork) |

![](https://static.igem.org/mediawiki/2016/thumb/e/e8/T--Groningen--week35-2.jpg/450px-T--Groningen--week35-2.jpg)

#### Week 36: Sep 11 – Sep 17

|  |  |
| --- | --- |
| HP | BBQ/Auction/ New Scientist interview/Shooting Unifocus movie |
| Modelling/Coding | Final Fixes everywhere for Wiki |
| Coding/Wiki | Wiki |
| Kill switch/Lab | Sequencing |
| Antibiotics/Lab | Ciprofloxacin-resistant B. sub. by directed evolution |
| RIVM - Safe by Design | Working on product of Proposal (booklet) Safe by Design |
| CRISPR/CAS9 | Start cloning first steps in lab |
| decoy/sfGFP | plan sfGFP biobrick |

![](https://static.igem.org/mediawiki/2016/thumb/2/2d/T--Groningen--Notebook-sfgfp-ecoli.jpg/800px-T--Groningen--Notebook-sfgfp-ecoli.jpg)

#### Week 37: Sep 18 – Sep 24

|  |  |
| --- | --- |
| Coding/Wiki | Wiki |
| Antibiotics/Lab | Ciprofloxacin-resistant B. sub. by directed evolution (experiment 2) |
| RIVM - Safe by Design | RIVM Meeting, Den Haag > Presentation of Product, Safe by Design |
| CRISPR/CAS9 | Start cloning first steps in lab |

![](https://static.igem.org/mediawiki/2016/thumb/0/09/T--Groningen--week37.jpg/800px-T--Groningen--week37.jpg)

#### Week 38: Sep 25 – Oct 1

|  |  |
| --- | --- |
| General | Portrait Picture |
| Coding/Wiki | Wiki |
| Integration/Lab | K823023 BioBrick received from iGEM headquarters |
| Kill switch/Lab | Start over clone RBS+nucA in pSB1C3, |
| Antibiotics/Lab | decoy experiment, clone PatpI promoter from gblock in pSB1C3 |
| Collaboration Wageningen | final plate reader experiment |
| CRISPR/CAS9 | The practialside is withdrawn |
| decoy/sfGFP | clone sfGFP in pSB1C3, sequencing |

![](https://static.igem.org/mediawiki/2016/thumb/9/98/T--Groningen--Notebook-take-pic.jpg/800px-T--Groningen--Notebook-take-pic.jpg)

#### Week 39: Oct 2 – Oct 8

|  |  |
| --- | --- |
| HP | Students from High School in NL BioART |
| Coding/Wiki | Wiki chill |
| Integration/Lab | clone key, message in pSB1C3, and K823023 |
| Kill switch/Lab | clone tetR+RBS+nucA in pSB1C3, send RBSnucA for sequencing |
| Antibiotics/Lab | sequence PatpI |
| decoy/sfGFP | decoy experiment FACS, microscopy |

![](https://static.igem.org/mediawiki/2016/thumb/c/cf/T--Groningen--week39.jpg/800px-T--Groningen--week39.jpg)

#### Week 40: Oct 9 – Oct 15

|  |  |
| --- | --- |
| General | New team photo |
| HP | Publication in the national news (NOS) |
| Coding/Wiki | Wiki cool |
| Integration/Lab | integrate message, key in B. subtili 168, strach test |
| Kill switch/Lab | clone tetR+RBS+nucA+TER2 in pSB1C3, find incorrect suffix in nucA K729004, order primer nucA correct prefix, suffix |
| Antibiotics/Lab | MIC test - E. coli wt vs qnrS1 |

![](https://static.igem.org/mediawiki/2016/b/b4/T--Groningen--Notebook-bara-eike-lab.jpg)

#### Week 41: Oct 16 – Oct 22

|  |  |
| --- | --- |
| HP | Interview Dagblad van het Noorden (local newspaper) |
| Coding/Wiki | Wiki freeze! |
| Kill switch/Lab | improve nucA clone it in pSB1C3 with correct prefix and suffix |
| Antibiotics/Lab | MIC tests - B. subtilis 168 (wt) vs (qnrS1) vs (resistant isolate) |

![](https://static.igem.org/mediawiki/2016/thumb/0/0d/T--Groningen--Notebook-biobricks.jpg/450px-T--Groningen--Notebook-biobricks.jpg)

#### Week 42: Oct 23 – Oct 29

|  |  |
| --- | --- |
| General | Jamboree! |

![](https://static.igem.org/mediawiki/2016/thumb/a/ad/T--Groningen--week42.jpg/800px-T--Groningen--week42.jpg)

## From our iGEM lab to the office

[

](https://static.igem.org/mediawiki/2016/3/3b/T--Groningen--Results-labtooffice.mp4)

From our iGEM lab to the office

[![Oop top](https://static.igem.org/mediawiki/2016/b/bd/T--Groningen--icon-up-bleu.png "Scroll to top")](#top)

---

###### Team

* [Members](/Team%3AGroningen/Team)
* [Supervisors](/Team%3AGroningen/Supervisors)
* [Collaborations](/Team%3AGroningen/Collaborations)
* [Achievements](/Team%3AGroningen/Achievements)
* [Contact](/Team%3AGroningen/Contact)
* [Jamboree](/Team%3AGroningen/Jamboree)

###### Project

* [Tour](/Team%3AGroningen/Tour)
* [Description](/Team%3AGroningen/Description)
* [Design](/Team%3AGroningen/Design)
* [Demonstration](/Team%3AGroningen/Demonstrate)
* [Summary](/Team%3AGroningen/Summary)
* [Applications](/Team%3AGroningen/Applications)
* [Results](/Team%3AGroningen/Results)
* [Future Perspectives](/Team%3AGroningen/FuturePerspectives)

###### Biology

* [Experiments](/Team%3AGroningen/Experiments)
* [Safety](/Team%3AGroningen/Safety)
* [Decoy experiment](/Team%3AGroningen/Decoy)
* [Photoswitchable antibiotics](/Team%3AGroningen/PhotoswitchableAntibiotics)
* [BioBrick Characterization](/Team%3AGroningen/BrickCharacter)
* [Key deletion](/Team%3AGroningen/KeyDeletion)

* [Proof of concept](/Team%3AGroningen/Proof)
* [Lab journal](/Team%3AGroningen/Labjournal)
* [Plasmid construction](/Team%3AGroningen/PlasmidConstruction)
* [Protocols](/Team%3AGroningen/Protocols)
* [Primer list](/Team%3AGroningen/Labjournal/PrimerList)
* [Notebook](/Team%3AGroningen/Notebook)

###### Modelling

* [Modelling](/Team%3AGroningen/Model)
* [Mutation Rates](/Team%3AGroningen/MutationRates)
* [Random Mutation](/Team%3AGroningen/RandomMutation)
* [AI Agent model](/Team%3AGroningen/AIModel)
* [Decoding Fidelity](/Team%3AGroningen/DecodingFidelity)
* [Optimal Strain](/Team%3AGroningen/SpiroModel)

###### Computing

* [Software](/Team%3AGroningen/Software)
* [Encoder](/Team%3AGroningen/Encoding)
* [Decoder](/Team%3AGroningen/Decoding)
* [Full Coding](/Team%3AGroningen/FullCoding)

###### Human Practices

* [Human Practices](/Team%3AGroningen/Human_Practices)
* [Outreach](/Team%3AGroningen/HP/Outreach)
* [Social](/Team%3AGroningen/HP/Social)
* [Legal](/Team%3AGroningen/HP/Legal)
* [Future Scenarios](/Team%3AGroningen/HP/FutureScenarios)

* [Education](/Team%3AGroningen/HP/Education)
* [Integrated Practices](/Team%3AGroningen/Integrated_Practices)
* [Engagement](/Team%3AGroningen/Engagement)
* [Gold](/Team%3AGroningen/HP/Gold)
* [Silver](/Team%3AGroningen/HP/Silver)

###### Support

* [Sponsors](/Team%3AGroningen/Sponsors)
* [Attributions](/Team%3AGroningen/Attributions)

[![](https://static.igem.org/mediawiki/2016/b/bf/HQ_menu_logo.jpg)](https://2016.igem.org)