iGEM 2018 — Team WLC-Milwaukee

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:WLC-Milwaukee/Notebook

PLACEHOLDER

[![WLC-Milwaukee](https://static.igem.org/mediawiki/2018/7/7b/T--WLC-Milwaukee--wlcMilwaukeeIgemLogo.png "WLC-Milwaukee iGEM Home")](/Team%3AWLC-Milwaukee)

[PROJECT](/Team%3AWLC-Milwaukee/Description)

[Overview](%5CTeam%3AWLC-Milwaukee%5CDescription)
[Background](%5CTeam%3AWLC-Milwaukee%5CBackground)
[System Design](%5CTeam%3AWLC-Milwaukee%5CApplied_Design)
[Wet Lab](%5CTeam%3AWLC-Milwaukee%5CExperiments)
[Results](%5CTeam%3AWLC-Milwaukee%5CDemonstrate)
[Parts](%5CTeam%3AWLC-Milwaukee%5CImprove)

[DOCUMENTATION](%5CTeam%3AWLC-Milwaukee%5CJudging)

[Safety](%5CTeam%3AWLC-Milwaukee%5CSafety)
[Timeline](%5CTeam%3AWLC-Milwaukee%5CNotebook)
[Judging](%5CTeam%3AWLC-Milwaukee%5CJudging)

[HUMAN PRACTICES](%5CTeam%3AWLC-Milwaukee%5CHP)

[Overview](%5CTeam%3AWLC-Milwaukee%5CHP)
[Investigations and Impact](%5CTeam%3AWLC-Milwaukee%5CHuman_Practices)
[Outreach](%5CTeam%3AWLC-Milwaukee%5CPublic_Engagement)
[Collaborations](%5CTeam%3AWLC-Milwaukee%5CCollaborations)

[TEAM](%5CTeam%3AWLC-Milwaukee%5CTeam)

[Members](%5CTeam%3AWLC-Milwaukee%5CTeam)
[Attributions](%5CTeam%3AWLC-Milwaukee%5CAttributions)

[![WLC-Milwaukee](https://static.igem.org/mediawiki/2018/7/7b/T--WLC-Milwaukee--wlcMilwaukeeIgemLogo.png "WLC-Milwaukee iGEM Home")
WLC-Milwaukee iGEM](/Team%3AWLC-Milwaukee)

*insert\_chart*
PROJECT

[Overview](%5CTeam%3AWLC-Milwaukee%5CDescription)
[Background](%5CTeam%3AWLC-Milwaukee%5CBackground)
[System Design](%5CTeam%3AWLC-Milwaukee%5CApplied_Design)
[Wetlab](%5CTeam%3AWLC-Milwaukee%5CExperiments)
[Results](%5CTeam%3AWLC-Milwaukee%5CDemonstrate)
[Parts](%5CTeam%3AWLC-Milwaukee%5CImprove)

*developer\_board*
DOCUMENTATION

[Safety](%5CTeam%3AWLC-Milwaukee%5CSafety)
[Timeline](%5CTeam%3AWLC-Milwaukee%5CNotebook)
[Judging](%5CTeam%3AWLC-Milwaukee%5CJudging)

*nature\_people*
HUMAN PRACTICES

[Overview](%5CTeam%3AWLC-Milwaukee%5CHP)
[Investigations and Impact](%5CTeam%3AWLC-Milwaukee%5CHuman_Practices)
[Outreach](%5CTeam%3AWLC-Milwaukee%5CPublic_Engagement)
[Collaborations](%5CTeam%3AWLC-Milwaukee%5CCollaborations)

*people*
TEAM

[Members](%5CTeam%3AWLC-Milwaukee%5CTeam)
[Attributions](%5CTeam%3AWLC-Milwaukee%5CAttributions)

[![Facebook](https://static.igem.org/mediawiki/2018/f/f5/T--WLC-Milwaukee--facebook.png)](https://www.facebook.com/WLCiGEM)

WLC iGEM 2018 | Timeline

![](https://static.igem.org/mediawiki/2018/9/9e/T--WLC-Milwaukee--TimelineHeader.jpeg)

# Project Timeline

Summarized below is the sequence of events that led to the completion of our project, from brainstorming ideas to finally submitting our parts and finishing the wiki!

## Project Decisions!

#### February 2018

![Parts](https://static.igem.org/mediawiki/2018/8/8e/T--WLC-Milwaukee--ProjectDecisions.jpeg)

---

Based on Judge feedback and our desire to bring the 2017 WLC-Milwaukee project to fruition, we decided to continue our 2017 project detecting *E. coli* in contaminated water supplies using Lambda Phage tail proteins. We began laying out the ways we would improve our past project and complete the areas where it had fallen short and been unsuccessful in 2017.

## Began Cloning parts into pTrc99a

#### March 2018

![Parts](https://static.igem.org/mediawiki/2018/1/12/T--WLC-Milwaukee--StartedCloning.jpeg)

---

With the help of our advisor, we designed primers and began the frustrating process of amplifying and cloning our parts from the pETail4 plasmid (containing the Lambda Phage tail protein genes) into pTrc99a in order to control protein expression levels using the pTrc99a IPTG inducible trc-promoter (also called Ptac).

## MUBRC

#### April 2018

---

A number of our team members participated in MUBRC (The Milwaukee Undergraduate Biological Research Conference) by volunteering and helping facilitate setup for this event while Jack presented a poster of our past work and hopeful project improvements in order to gain feedback from other undergraduate researchers and PIs in attendance.

## Started Human Practices Investigation

#### June 2018

![Parts](https://static.igem.org/mediawiki/2018/3/31/T--WLC-Milwaukee--MicroscopeGraphic.png)

---

As a team we began investigating issues relating to water quality and testing, how our project may fit into the market, how our project could fulfill a need for water testing in certain areas, and could be designed to reduce waste and eliminate any environmental hazards.

## Organization Outreach Day

#### August 2018

---

In August Jack and Laura presented a poster and posterboard at the Organization Outreach day, welcoming new freshman students to our campus and talking with various students about bioengineering, synbio, and our project. Through this event we made contact with a number of students who were very interested in joining iGEM and have already gotten involved with our team!

## Confirmed Clones in pTrc99a

#### September 2018

---

We finally obtained our clones in the pTrc99a backbone and confirmed their presence by diagnostic digest and sequencing. This was a major step as we could now clone our parts into pSB1C3 and include the trc-promoter for IPTG induction in our parts.

## Science Exploration Day

#### September 2018

![Parts](https://static.igem.org/mediawiki/2018/b/b2/T--WLC-Milwaukee--ScienceExplorationDay.jpeg)

---

This event was a great Outreach event and was an awesome way to interact both with almost 20 grade school aged students and their parents from the local community and provide various lab activities and opportunities to these bright young students. The students participated in hands on explorations of some of the numerous areas within STEM.

## Protein Purification and Analysis

#### October 2018

![Parts](https://static.igem.org/mediawiki/2018/3/39/T--WLC-Milwaukee--ProteinPurification.jpeg)

---

After obtaining successful clones of our parts into pSB1C3 we were finally able to start protein purification using Nickel column purification methods and analyze our parts for successful protein production.

## Bacteriophages for High Schoolers

#### October 2018

![Parts](https://static.igem.org/mediawiki/2018/f/f9/T--WLC-Milwaukee--VirusesforHighschoolers.jpeg)

---

After analyzing substrate-HRP ratios and which methods would suit our test kit system, we began a system prototype, conjugated HRP to our proteins, and performed some testing to optimize system operation both in controlled ideal lab circumstances and in more realistic circumstances to determine the viability of our product.

## Submitted Parts and finished the Wiki!

#### October 2018

![Parts](https://static.igem.org/mediawiki/2018/7/74/T--WLC-Milwaukee--SubmittingParts.jpeg)

---

We submitted our parts to the Registry and started working on finishing our Wiki, confirming our results, and completing our project prototype!

## Young Women in Science Program

#### March 3rd 2018

![Parts](https://static.igem.org/mediawiki/2018/a/ab/T--WLC-Milwaukee--YWSPpic.png)

---

We were able to continue our relationship with the Young Women in Science Program (YWSP) providing lab activities for 13 young women interested in STEM examining bacterial membranes, membrane permeability, and bioengineering in the real world.

## Planned Outreach Events

#### April 2018

---

We began laying out and deciding on some of our larger scale Outreach events for the fall and considering additional ways we could engage and educate our surrounding community in a holistic way.

## New Cloning Primers

#### May 2018

![Parts](https://static.igem.org/mediawiki/2018/6/61/T--WLC-Milwaukee--NewPrimers.jpeg)

---

Due to what appeared to be inconsistencies in our gene amplification we added a single base onto some of our cloning primers and increased our annealing time which allowed for better gene amplification and aiding in our cloning efforts.

## Jack Tours Cornell Lab Spaces

#### August 2018

![Parts](https://static.igem.org/mediawiki/2018/1/1d/T--WLC-Milwaukee--WeillHall.jpeg)

---

Over the summer, our team lead Jack was able to attend the Cornell SILS (Summer Institute of Life Sciences) symposium and meet members of the Cornell iGEM Team. He was able to speak with some of their members, particularly their team lead Rahul and tour their lab spaces. We greatly appreciate the openness and collaborations of the Cornell iGEM Team and Jack enjoyed seeing what it would be like to have an entire lab space dedicated to iGEM research!

## Spoke with Experts to Analyze our System

#### September 2018

![Parts](https://static.igem.org/mediawiki/2018/2/24/T--WLC-Milwaukee--Experts.jpeg)

---

In September we began communicating with experts in environmental testing and the water safety industry. Due to their feedback we modified our initial project design to make it more user friendly, and altered the intended scope of our project application after re-analyzing the testing systems in place within the large scale water management industry.

## Tested HRP and Substrates based on new system goals

#### September 2018

![Parts](https://static.igem.org/mediawiki/2018/c/c1/T--WLC-Milwaukee--HRPGraphicNEW.png)

---

After re-examining our system impact and applicability we also needed to change the substrates and ratio of HRP-Substrate we had been intending on using with our test kit to better suit the niche our kit best fills. Based on these changes we began testing new combinations and concentrations of our substrates and HRP to optimize our kit in terms of both efficiency and accuracy.

## Obtained clones in pSB1C3

#### October 2018

![Parts](https://static.igem.org/mediawiki/2018/e/e1/T--WLC-Milwaukee--ClonesinpSB1C3.jpeg)

---

After much frustration we finally succeeded in obtaining successful clones of our parts both with and without the trc-promoter (also known as Ptac) in pSB1C3!

## Visited MMSD treatment plant

#### October 2018

![Parts](https://static.igem.org/mediawiki/2018/6/6a/T--WLC-Milwaukee--WastewaterTreatmentVisit.jpeg)

---

As another layer to our system design investigations we visited and toured the Milwaukee Municipal Sewer District (MMSD) wastewater treatment plant to learn whether or not our project would be viable on the large scale of Municipal Sewer Effluent management. Additionally, we sought to compare how our project could be used at small scale wastewater treatment facilities versus massive facilities such as the MMSD facilities in the Milwaukee area.

## System Prototyping and Continued Testing

#### October 2018

![Parts](https://static.igem.org/mediawiki/2018/5/5b/T--WLC-Milwaukee--PrototypeTesting.jpeg)

---

After analyzing substrate-HRP ratios and which methods would suit our test kit system, we began a system prototype, conjugated HRP to our proteins, and performed some testing to optimize system operation both in controlled ideal lab circumstances and in more realistic circumstances to determine the viability of our product.

* [Project](%5CTeam%3AWLC-Milwaukee%5CDescription)
* [Background](%5CTeam%3AWLC-Milwaukee%5CBackground)
* [System Design](%5CTeam%3AWLC-Milwaukee%5CApplied_Design)
* [Wet Lab](%5CTeam%3AWLC-Milwaukee%5CExperiments)
* [Results](%5CTeam%3AWLC-Milwaukee%5CDemonstrate)
* [Parts](%5CTeam%3AWLC-Milwaukee%5CImprove)

* [Documentation](%5CTeam%3AWLC-Milwaukee%5CNotebook)
* [Safety](%5CTeam%3AWLC-Milwaukee%5CSafety)
* [Timeline](%5CTeam%3AWLC-Milwaukee%5CNotebook)
* [Judging](%5CTeam%3AWLC-Milwaukee%5CJudging)

* [Human Practices](%5CTeam%3AWLC-Milwaukee%5CHP)
* [Investigations & Impact](%5CTeam%3AWLC-Milwaukee%5CHuman_Practices)
* [Outreach](%5CTeam%3AWLC-Milwaukee%5CPublic_Engagement)
* [Collaborations](%5CTeam%3AWLC-Milwaukee%5CCollaborations)

* [Team](%5CTeam%3AWLC-Milwaukee%5CTeam)
* [Members](%5CTeam%3AWLC-Milwaukee%5CTeam)
* [Attributions](%5CTeam%3AWLC-Milwaukee%5CAttributions)

![Wisconsin Lutheran College](https://static.igem.org/mediawiki/2018/7/79/T--WLC-Milwaukee--WLC.png)


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:WLC-Milwaukee/Experiments

PLACEHOLDER

[![WLC-Milwaukee](https://static.igem.org/mediawiki/2018/7/7b/T--WLC-Milwaukee--wlcMilwaukeeIgemLogo.png "WLC-Milwaukee iGEM Home")](/Team%3AWLC-Milwaukee)

[PROJECT](/Team%3AWLC-Milwaukee/Description)

[Overview](%5CTeam%3AWLC-Milwaukee%5CDescription)
[Background](%5CTeam%3AWLC-Milwaukee%5CBackground)
[System Design](%5CTeam%3AWLC-Milwaukee%5CApplied_Design)
[Wet Lab](%5CTeam%3AWLC-Milwaukee%5CExperiments)
[Results](%5CTeam%3AWLC-Milwaukee%5CDemonstrate)
[Parts](%5CTeam%3AWLC-Milwaukee%5CImprove)

[DOCUMENTATION](%5CTeam%3AWLC-Milwaukee%5CJudging)

[Safety](%5CTeam%3AWLC-Milwaukee%5CSafety)
[Timeline](%5CTeam%3AWLC-Milwaukee%5CNotebook)
[Judging](%5CTeam%3AWLC-Milwaukee%5CJudging)

[HUMAN PRACTICES](%5CTeam%3AWLC-Milwaukee%5CHP)

[Overview](%5CTeam%3AWLC-Milwaukee%5CHP)
[Investigations and Impact](%5CTeam%3AWLC-Milwaukee%5CHuman_Practices)
[Outreach](%5CTeam%3AWLC-Milwaukee%5CPublic_Engagement)
[Collaborations](%5CTeam%3AWLC-Milwaukee%5CCollaborations)

[TEAM](%5CTeam%3AWLC-Milwaukee%5CTeam)

[Members](%5CTeam%3AWLC-Milwaukee%5CTeam)
[Attributions](%5CTeam%3AWLC-Milwaukee%5CAttributions)

[![WLC-Milwaukee](https://static.igem.org/mediawiki/2018/7/7b/T--WLC-Milwaukee--wlcMilwaukeeIgemLogo.png "WLC-Milwaukee iGEM Home")
WLC-Milwaukee iGEM](/Team%3AWLC-Milwaukee)

*insert\_chart*
PROJECT

[Overview](%5CTeam%3AWLC-Milwaukee%5CDescription)
[Background](%5CTeam%3AWLC-Milwaukee%5CBackground)
[System Design](%5CTeam%3AWLC-Milwaukee%5CApplied_Design)
[Wetlab](%5CTeam%3AWLC-Milwaukee%5CExperiments)
[Results](%5CTeam%3AWLC-Milwaukee%5CDemonstrate)
[Parts](%5CTeam%3AWLC-Milwaukee%5CImprove)

*developer\_board*
DOCUMENTATION

[Safety](%5CTeam%3AWLC-Milwaukee%5CSafety)
[Timeline](%5CTeam%3AWLC-Milwaukee%5CNotebook)
[Judging](%5CTeam%3AWLC-Milwaukee%5CJudging)

*nature\_people*
HUMAN PRACTICES

[Overview](%5CTeam%3AWLC-Milwaukee%5CHP)
[Investigations and Impact](%5CTeam%3AWLC-Milwaukee%5CHuman_Practices)
[Outreach](%5CTeam%3AWLC-Milwaukee%5CPublic_Engagement)
[Collaborations](%5CTeam%3AWLC-Milwaukee%5CCollaborations)

*people*
TEAM

[Members](%5CTeam%3AWLC-Milwaukee%5CTeam)
[Attributions](%5CTeam%3AWLC-Milwaukee%5CAttributions)

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WLC iGEM 2018 | Wetlab

# Wet Lab

![](https://static.igem.org/mediawiki/2018/5/55/T--WLC-Milwaukee--WetLabHeader.jpeg)

Our Wet Lab efforts focused on first designing our parts in such a way as to test new versions of past WLC-iGEM binding proteins for optimization and use in our test kit. Our work then turned to successfully sub-cloning our new parts ([BBa\_K2589000](http://parts.igem.org/Part%3ABBa_K2589000), [BBa\_K2589002](http://parts.igem.org/wiki/index.php?title=Part:BBa_K2589002)) from the pETail4 plasmid containing the lambda phage genome into both pTrc99a and pSB1C3 along with improving a past part [BBa\_K2452002](http://parts.igem.org/Part%3ABBa_K2452002) to create the composite part [BBa\_K2589001](http://parts.igem.org/wiki/index.php?title=Part:BBa_K2589001). Our final focus in WetLab was to purify the proteins from our new parts using Nickel column purification (as most of our parts contain a His tag element) and then confirming expression or protein presence.

![](https://static.igem.org/mediawiki/2018/5/56/T--WLC-Milwaukee--iGembacteriapairwikigraphic.png)

# Part Design

Our Wet Lab team and Dr. Werner met early in the year to discuss the full spectrum of possible parts we would attempt to create and submit, and as with many iGEM teams we set our goals a bit too high, hoping to create a series of fusion proteins with which to better analyze our test kit on top of the basic parts needed for our system. We analyzed the sequence of the pTrc99a plasmid and decided to use the trc-promoter [BBa\_K2042004](http://parts.igem.org/Part%3ABBa_K2042004) for induction of all of our lambda phage tail protein parts with IPTG. Because the part sample for this promoter has not been released, primers had to be designed to input our parts into the pTrc99a backbone and then further clone our parts into pSB1C3 with the trc-promoter from the pTrc99a backbone.

The basic parts we designed at the beginning of our project include [BBa\_K2589000](http://parts.igem.org/Part%3ABBa_K2589000) and [BBa\_K2589002](http://parts.igem.org/wiki/index.php?title=Part:BBa_K2589002), and an improved composite part [BBa\_K2589001](http://parts.igem.org/wiki/index.php?title=Part:BBa_K2589001) consisting of [BBa\_K2452002](http://parts.igem.org/Part%3ABBa_K2452002) and [BBa\_K2042004](http://parts.igem.org/Part%3ABBa_K2042004). Dr. Werner helped us design our primers in a major way ensuring our sequences were accurate and should work as expected. We did run into some problems with low gene amplification during PCR and ended up increasing the size of our original primers by 1bp.

# Cloning

We followed standard procedures for the cloning of our parts, first amplifying our genes of interest by PCR, digesting and ligating our parts into our backbone(s), and transformation via electroporation into electrocompetent lab strain *E. coli*. Successful transformants underwent initial screening through diagnostic restriction enzyme digests to confirm the presence of our plasmid backbones and appropriate genes.

![image](https://static.igem.org/mediawiki/2018/5/5e/T--WLC-Milwaukee--ResultsHeaderNEW.jpeg)

When diagnostic digests showed high potential for a successful clone, samples were sent for sequencing to (in most cases) confirm the presence and accurate sequence of our parts. We ran into numerous issues during cloning that slowed the progression of our lab work and project including poor gene amplification during PCR which was inevitably solved using slightly longer primers and a longer annealing time. Additionally, we had some unexpected initial sequencing data that confounded the situation, however, after additional screening we believe our part sequences to be accurate and complete. See [Results](https://2018.igem.org/Team%3AWLC-Milwaukee/Demonstrate) for data and the results of our cloning

# Protein Expression and Purification

After confirmation of successful cloning of our parts, we began the process of protein purification. Strains with successful clones were cultured and then diluted and induced using IPTG for maximal protein expression. Our proteins were purified using a Nickel column purification kit/system from Qiagen (Ni-NTA Fast Start Kit) after which a series of tests (some successful some not) were performed in an attempt to confirm the presence and functionality of our purified proteins, including PAGE gels with Coomassie Staining. We then proceeded to conjugate Horseradish peroxidase to our protein samples and performed His-tag specific Western Blotting and chemiluminescent tests (using our parts combined with various diluted cultures of *E.coli* and exposed to a chemiluminescent substrate cleaved by HRP) to confirm the purification, conjugation of HRP to, and binding of, our proteins to *E.coli*. To find more information regarding our testing please see [Results](https://2018.igem.org/Team%3AWLC-Milwaukee/Demonstrate) and [System Design](https://2018.igem.org/Team%3AWLC-Milwaukee/Applied_Design).

* [Project](%5CTeam%3AWLC-Milwaukee%5CDescription)
* [Background](%5CTeam%3AWLC-Milwaukee%5CBackground)
* [System Design](%5CTeam%3AWLC-Milwaukee%5CApplied_Design)
* [Wet Lab](%5CTeam%3AWLC-Milwaukee%5CExperiments)
* [Results](%5CTeam%3AWLC-Milwaukee%5CDemonstrate)
* [Parts](%5CTeam%3AWLC-Milwaukee%5CImprove)

* [Documentation](%5CTeam%3AWLC-Milwaukee%5CNotebook)
* [Safety](%5CTeam%3AWLC-Milwaukee%5CSafety)
* [Timeline](%5CTeam%3AWLC-Milwaukee%5CNotebook)
* [Judging](%5CTeam%3AWLC-Milwaukee%5CJudging)

* [Human Practices](%5CTeam%3AWLC-Milwaukee%5CHP)
* [Investigations & Impact](%5CTeam%3AWLC-Milwaukee%5CHuman_Practices)
* [Outreach](%5CTeam%3AWLC-Milwaukee%5CPublic_Engagement)
* [Collaborations](%5CTeam%3AWLC-Milwaukee%5CCollaborations)

* [Team](%5CTeam%3AWLC-Milwaukee%5CTeam)
* [Members](%5CTeam%3AWLC-Milwaukee%5CTeam)
* [Attributions](%5CTeam%3AWLC-Milwaukee%5CAttributions)

![Wisconsin Lutheran College](https://static.igem.org/mediawiki/2018/7/79/T--WLC-Milwaukee--WLC.png)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:WLC-Milwaukee/Results

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[HOME](https://2018.igem.org/Team%3AWLC-Milwaukee)

TEAM

[Team Members](https://2018.igem.org/Team%3AWLC-Milwaukee/Team)
[Collaborations](https://2018.igem.org/Team%3AWLC-Milwaukee/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AWLC-Milwaukee/Description)
[Design](https://2018.igem.org/Team%3AWLC-Milwaukee/Design)
[Experiments](https://2018.igem.org/Team%3AWLC-Milwaukee/Experiments)
[Notebook](https://2018.igem.org/Team%3AWLC-Milwaukee/Notebook)
[InterLab](https://2018.igem.org/Team%3AWLC-Milwaukee/InterLab)
[Model](https://2018.igem.org/Team%3AWLC-Milwaukee/Model)
[Results](https://2018.igem.org/Team%3AWLC-Milwaukee/Results)
[Demonstrate](https://2018.igem.org/Team%3AWLC-Milwaukee/Demonstrate)
[Improve](https://2018.igem.org/Team%3AWLC-Milwaukee/Improve)
[Attributions](https://2018.igem.org/Team%3AWLC-Milwaukee/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AWLC-Milwaukee/Parts)
[Basic Parts](https://2018.igem.org/Team%3AWLC-Milwaukee/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AWLC-Milwaukee/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AWLC-Milwaukee/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AWLC-Milwaukee/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AWLC-Milwaukee/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AWLC-Milwaukee/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AWLC-Milwaukee/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AWLC-Milwaukee/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AWLC-Milwaukee/Hardware)
[Measurement](https://2018.igem.org/Team%3AWLC-Milwaukee/Measurement)
[Model](https://2018.igem.org/Team%3AWLC-Milwaukee/Model)
[Plant](https://2018.igem.org/Team%3AWLC-Milwaukee/Plant)
[Software](https://2018.igem.org/Team%3AWLC-Milwaukee/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=WLC-Milwaukee)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)