iGEM 2015 — Team Freiburg

# Team:Freiburg/Notebook

[![](https://static.igem.org/mediawiki/2015/9/93/Freiburg_upwards_blob.png)](#globalWrapper)

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* [Home](https://2015.igem.org/Team%3AFreiburg/Home)
* [Project](https://2015.igem.org/Team%3AFreiburg/Project/Overview)
* [Team](https://2015.igem.org/Team%3AFreiburg/Team)
* [Results](https://2015.igem.org/Team%3AFreiburg/Results)
* [Human Practice](https://2015.igem.org/Team%3AFreiburg/Practices)
* [Notebook](https://2015.igem.org/Team%3AFreiburg/Notebook)

* [Introduction](https://2015.igem.org/Team%3AFreiburg/Project/Overview)
* [Overview](https://2015.igem.org/Team%3AFreiburg/Project/System)
* [Diagnostics today](https://2015.igem.org/Team%3AFreiburg/Design)
* The DiaCHIP
  + [DNA Engineering](https://2015.igem.org/Team%3AFreiburg/Project/DNA_Engineering)
  + [Cell-Free Expression](https://2015.igem.org/Team%3AFreiburg/Project/Cellfree_Expression)
  + [Protein Purification](https://2015.igem.org/Team%3AFreiburg/Project/Protein_Purification)
  + [Surface Chemistry](https://2015.igem.org/Team%3AFreiburg/Project/Surface_Chemistry)
  + [Optical Detection](https://2015.igem.org/Team%3AFreiburg/Project/iRIf)
* [Outlook](https://2015.igem.org/Team%3AFreiburg/Project/Future_Directions)

* [Members](https://2015.igem.org/Team%3AFreiburg/Team)
* [Acknowledgements](https://2015.igem.org/Team%3AFreiburg/Attributions)
* [Partners](https://2015.igem.org/Team%3AFreiburg/Partners)

* The DiaCHIP
  + [Main Results](https://2015.igem.org/Team%3AFreiburg/Results)
  + [Diagnostics](https://2015.igem.org/Team%3AFreiburg/Results/Diagnostics)
  + [Our Device](https://2015.igem.org/Team%3AFreiburg/Results/Own_Device)
  + [DNA Array](https://2015.igem.org/Team%3AFreiburg/Results/Immobilization)
  + [Cell-Free Expression](https://2015.igem.org/Team%3AFreiburg/Results/Cellfree)
  + [Binding on Surface](https://2015.igem.org/Team%3AFreiburg/Results/Surface)
  + [Modeling](https://2015.igem.org/Team%3AFreiburg/Results/Modeling)
* [BioBricks](https://2015.igem.org/Team%3AFreiburg/Parts)
* [Collaborations](https://2015.igem.org/Team%3AFreiburg/Collaborations)
* [Interlab Study](https://2015.igem.org/Team%3AFreiburg/InterLab_Study)
* [Achievements](https://2015.igem.org/Team%3AFreiburg/Achievements)

* [Science Fair](https://2015.igem.org/Team%3AFreiburg/Human_Practice/Science_Fair)
* [Theater Project](https://2015.igem.org/Team%3AFreiburg/Human_Practice/Theater_Project)
* [Public Opinion](https://2015.igem.org/Team%3AFreiburg/Human_Practice/Public_Opinion)
* [College Talk](https://2015.igem.org/Team%3AFreiburg/Human_Practice/College)
* [Ethics](https://2015.igem.org/Team%3AFreiburg/Human_Practice/Ethics)

* [Labjournals](https://2015.igem.org/Team%3AFreiburg/Labjournals)
* Techniques
  + [Gibson](https://2015.igem.org/Team%3AFreiburg/Project/Classic_vs_Gibson)
  + [E.coli Strains](https://2015.igem.org/Team%3AFreiburg/Project/Coli_Strains)
* [Protocols](https://2015.igem.org/Team%3AFreiburg/Protocols)
* [Materials](https://2015.igem.org/Team%3AFreiburg/Materials)
* [Safety](https://2015.igem.org/Team%3AFreiburg/Safety)
* [Wiki-Tricks](https://2015.igem.org/Team%3AFreiburg/Wikimigration)
* [Glossary](https://2015.igem.org/Team%3AFreiburg/Glossary)

* [Home](https://2015.igem.org/Team%3AFreiburg/Home)
* [Project](https://2015.igem.org/Team%3AFreiburg/Project/Overview)
* [Results](https://2015.igem.org/Team%3AFreiburg/Results)
* [Team](https://2015.igem.org/Team%3AFreiburg/Team)
* [Practice](https://2015.igem.org/Team%3AFreiburg/Practices)
* [Notebook](https://2015.igem.org/Team%3AFreiburg/Notebook)

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![](https://static.igem.org/mediawiki/2015/f/f4/Freiburg_wiki_background_2_parted_right.png)

# Labjournals

![](https://static.igem.org/mediawiki/2015/0/06/Freiburg_Notitzblock_scaled_transp.jpg)

Follow us step by step on our way to developing the DiaCHIP!

* [DNA Engineering](https://2015.igem.org/Team%3AFreiburg/Labjournals#cloning_lab)
* [Protein Purification](https://2015.igem.org/Team%3AFreiburg/Labjournals#protpur_lab)
* [Cell-free Expression](https://2015.igem.org/Team%3AFreiburg/Labjournals#cellfree_lab)
* [Surface Chemistry](https://2015.igem.org/Team%3AFreiburg/Labjournals#surchem_lab)
* [iRIf](https://2015.igem.org/Team%3AFreiburg/Labjournals#irif_lab)

Want to read about [the Interlab Study](https://2015.igem.org/Team%3AFreiburg/Labjournals/Interlab)?

![](https://static.igem.org/mediawiki/2015/e/e0/Freiburg_Stift_scaled_transp.jpg)

# [Protocols](https://2015.igem.org/Team%3AFreiburg/Protocols)

Interested in redoing our experiments yourself?

![](https://static.igem.org/mediawiki/2015/0/0f/Freiburg_Kolben_scaled_transp.jpg)

# [Materials](https://2015.igem.org/Team%3AFreiburg/Materials)

A list of all the chemicals, enzymes and machines we used during our iGEM project.

![](https://static.igem.org/mediawiki/2015/a/ac/Freiburg_Safety_scaled_transp.jpg)

# [Safety](https://2015.igem.org/Team%3AFreiburg/Safety)

Checkout all relevant information concerning the organisms we worked with!

![](https://static.igem.org/mediawiki/2015/f/fd/Freiburg_Zauberstab_scaled_transp.jpg)

# [Wiki-Tricks](https://2015.igem.org/Team%3AFreiburg/Wikimigration)

Find out about how we speeded up transfering our data on the external iGEM wiki

![](https://static.igem.org/mediawiki/2015/d/d8/Freiburg_schraubenschluessel.png)

# [Techniques](https://2015.igem.org/Team%3AFreiburg/Wikimigration)

Want more information on techniques we used?

* [Classical Cloning vs Gibson Cloning](https://2015.igem.org/Team%3AFreiburg/Project/Classic_vs_Gibson)
* [*E. coli genotypes* we used](https://2015.igem.org/Team%3AFreiburg/Project/Coli_Strains)

![](https://static.igem.org/mediawiki/2015/d/de/Freiburg_buch.jpg)

# [Glossary](https://2015.igem.org/Team%3AFreiburg/Glossary)

Not sure what a word means? Check here.

![](https://static.igem.org/mediawiki/2015/f/f6/Freiburg_footer_email.png)

[![](https://static.igem.org/mediawiki/2015/1/12/Freiburg_facebook_footer.png)](https://www.facebook.com/igemfreiburg15)

[![](https://static.igem.org/mediawiki/2015/d/d7/Freiburg_footer_bioss.png)](http://www.bioss.uni-freiburg.de/)

[![](https://static.igem.org/mediawiki/2015/c/c8/Freiburg_Zbsa-logo.png)](http://www.zbsa.uni-freiburg.de/)

[![](https://static.igem.org/mediawiki/2015/d/dd/Freiburg_footer_uni.png)](http://www.uni-freiburg.de/)