iGEM 2012 — Team Uppsala_University

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# Team:Uppsala University/Notebook

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Team Uppsala University – iGEM 2012

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Notebook

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| **Thursday 28 June**    Participant: Joel  Experiment: Gel Electrophoresis of yesterday's screening products  18.1-2 pSB1C3 – cat-sacB  19.1-2 pSB1C3 - sacB  20.1-2 BBa\_K157000 – Fok\_a  21.1-2 BBa\_K157000 – Fok\_i  Result: None of the band were correct    Participant: Caroline, Emy, Ellen, Joel  Experiment: Transformation  pSB3T5 - lacIs - TλT1  pSB3T5 - SYFP2 - TλT1  pSB3T5 - T5lac - B0034  Followed TOP10 transformation protocol    Participant: Fredrik, Julia, Joakim K, Olle  Experiment: Plasmid Prep and Glycerol Stock  18.1-2 pSB1C3 – cat-sacB  19.1-2 pSB1C3 - sacB  20.1-2 BBa\_K157000 – Fok\_a  21.1-2 BBa\_K157000 – Fok\_i  Followed Plasmid Purification Protocol    Participant: Lisa, Sabri  Experiment: o/n culture  15.1-2 pSB1A2 - Plac  17.1-2 pSB1A2 - PlacIQ    Participant: Joakim, Anna  Experiment: Make new SOB & SOC    Participant: Tobias, Christoffer  Experiment: Transformation  pEL3S15 - J23101    **Friday 29 June**    Participant: Julia, Hampus, Tobias  Experiment: Re-streak  pEL3S15 - J23101    Participant: Olle, Sabri, Lisa  Experiment: Transformation  23. pSB1A1 - P1004 (cat) from registry well 7B  24. pSB2K3 - tetR from registry well 16P    Participant: Sabri, Lisa, Josefin, Niklas  Experiment: Assembly  pSB1K3 - B0034 - SYFP2  pSB3C5 - J23101 - tet inv    Participant: Caroline, Emy, Ellen, Joel, Tobias  Experiment: Assembly  pSB3T5 - PlacQ - ACC(6')  pSB3T5 - PlacQ - CTX-M-15  pSB3T5 - PlacQ - tetA  pSB3K3 - Plac - B0034 - SYFP2  pSB1C3 - TλT1    Participant: Caroline, Emy, Ellen, Joel, Tobias  Experiment: Re-streak  pSB3T5 - lacIs - TλT1  pSB3T5 - SYFP2 - TλT1  pSB3T5 - T5lac - B0034    Participant: Anna, Ellen  Experiment: o/n culture  J23116    Participant: Anna, Ellen  Experiment: Plasmid Prep  15.1-2 pSB1A2 - Plac  17.1-2 pSB1A2 - PlacQ    **Saturday 30 June**    Participant: Hampus, Julia, Tobias  Experiment: Screening  pEL3S15 - J23101  pSB3T5 - lacIs -   pSB3T5 - SYFP2 -   pSB3T5 - pT5lac - B0034  Result: Bad gel    Participant: Caroline, Fredrik, Emy, Hampus  Experiment: o/n culture  30.1-4 pEL3S15 - J23101  31.1-4 pEL3T5 - lacIs -   32.1-4 pSB3T5 - SYFP2 -   33.1-2 pSB3T5- pT5lac - B0034  20.1 BBa\_K157000 - Fok\_a  21.1 BBa\_K157000 - Fok\_i    Participant: Ellen, Emy, Caroline, Joel  Experiment: Plasmid Prep  J61002 - J23106  J61002 - J23116    Participant: Ellen, Emy, Caroline, Joel  Experiment: Transformation  pSB3T5 - PlacIq - AAC(6')  pSB3T5 - PlacIq - CTX-M  pSB3T5 - PlacIq - tetA  pSB3K3 - Plac - B0034 - SYFP2  pSB1C3 - T....    Participant: Ellen, Emy, Caroline, Joel  Experiment: Assembly  pSB3T5 - J23106 - AAC(6')  pSB3T5 - J23106 - CTX-M  pSB3T5 - J23106 - tetA  pSB3T5 - J23116 - AAC(6')  pSB3T5 - J23116 - CTX-M  pSB3T5 - J23116 - tetA    Participant: Fredrik  Experiment: Transformation  24. pSB2K3 - tet inv  39. pSB1K3 - B0034 - SYFP2  40. pSB3C5 - J23101 - tet inv    Participant: Fredrik  Experiment: Re-streak  16. pSB2K3 - pBAD  23. pSB1A1 - P1004  24. pSB1K3 - tet inv    **Sunday 1 July**    Participant: Hampus, Julia, Lisa  Experiment: Ran new gel of yesterday's screening  pEL3S15 - J23101  pSB3T5 - lacIs -   pSB3T5 - SYFP2 -   pSB3T5 - pT5lac - B0034  Result: Contamination    Participant: Caroline, Emy  Experiment: Transformation  pSB3T5 - J23106 - AAC(6')  pSB3T5 - J23106 - CTX-M  pSB3T5 - J23106 - tetA  pSB3T5 - J23116 - AAC(6')  pSB3T5 - J23116 - CTX-M  pSB3T5 - J23116 - tetA    Participant: Caroline, Emy  Experiment: Re-streak  pSB3T5 - PlacIq - AAC(6')  pSB3T5 - PlacIq - CTX-M  pSB3T5 - PlacIq - tetA  pSB3K3 - Plac - B0034 - SYFP2  pSB1C3 - T....    Participant: Emy, Caroline, Joel  Experiment: o/n culture  31.1-4 pSB3T5 - lacIs - T...  32.1-4 pSB3T5 - SYFP2 - T...  33.1-2 pSB3T5 - pT5lac - B0034  24.1-4 pSB1K3 - tet inv  30.1-4 pEL3S15 - J23101  20.1 BBa\_K157000 - Fok\_a  21.2 BBa\_K157000 - Fok\_i  23.1-4 pSB1A1 - P1004 (BBa\_P1004)  44. pEL3C15 (FRT) - Red  45. pEL3C15 - Red  46. pEL3A15 - Red  47. pEL3A15 (FRT) - Red  48. pEL3S15 (FRT) - Red  49. pEL3S15 - Red  50. pEL3K15 (FRT) - Red  51. pEL3K15 - Red    Participant: Joel  Experiment: Transformation from kit  41. pSB2K3 - Pcat (BBa\_I14033) from plate 1, well 18P  42. pSB2K3 - pBAD (BBa\_I14018) from plate 1, well 18N  43. pSB2K3 - Pcat (BBa\_I14031) from plate 2, well 13B    **Monday 9 July**    Participant: Hampus, Christofer  Experiment: Screening  33. pSB1A3 - PT5lac - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034  Result: 33, 80 and 81 showed correct bands.    Participant: Anton, Joakim, Karl  Experiment: Prepare for sequencing  40. pSB3C5 - J23101 - tet inv.  64 pEL3S15 - J23101 - Spot42    Experiment: O/N  82. pSB1AK3 - Plac - B0034  12. pSB1A2 - B0034  83. pUC57 - TλT1    Experiment: Assembly  39. pSB1K3 - B0034 - SYFP2    Experiment: Cloning  88. pSB1C3 - Pcat    Participant: Emy, Ellen  Experiment: Assembly and transformation  109. pEL3A15 - PlacIq - AAC(6') - lacIs  110. pEL3A15 - PlacIq - CTX-M - lacIs  111. pEL3A15 - PlacIq - tet-A - lacIs    Participant: Lisa, Sabri  Experiment: Plasmid prep.  64. pEL3S15 - J23101 - Spot42    Participant: Julia, Hampus  Experiment: Amplify SpecA-casette from pEL3S15 - J23101 - Spot42    **Tuesday 10 July**    Participant: Joel Ås  Experiment: Transform  109. pEL3A15- PlacIq - AAC(6') - lacIs  110. pEL3A15 - PlacIq - CTX-M - lacIs  111. pEL3A15 - PlacIq - tetA - lacIs    Participant: Anna, Emy  Experiment: Assembly and Transformation  113. pSB3T5 - J23110 - AAC(6')  114. pSB3T5 - J23110 - CTX-M    Participant: Anna, Emy, Karl  Experiment: Streak from frozen stock  9. pSB1C3 - CTX-M    Experiment: O/N from frozen stock  3. pSB3T5 - red    Participant: Olle  Experiment: Assembly  59. pSB3T5 - J23116 - tetA  115. pSB3T5 - J23110 - tetA    Experiment: Transform  59. pSB3T5 - J23116 - tetA  91. pSb1C3 - marR  115. pSB3T5 - J23110 - tetA    Participant: Arvid  Experiment: Control experiment  Since we've had problems growing pSB3K3-pBAD/acaC on a K-backbone, we tested some different antibiotics  Result: Someone probably mixed up the sample at an early stage since it grew in ampicillin.    Experiment: Plasmid prep and Frozen Stock  12. pSB1A2 - B0034  33. pSB3T5 - T5lac - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034  82. pSB1AK3 - Plac - B0034  83. pUC57 - TλT1    Participant: Anton Berglund  Experiment: Transformation  39. pSB1K3 - B0034 - SYFP2  88. pSB1C3 - Pcat    Experiment: Prep for sequencing  33. pSB3T5 - PT5lac - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034  82. pSB1AK3 - Plac - B0034  84. pSB3T5 - cat-sacB    Participant: Johan  Experiment: re-streak  pSB1C3 - marR (BioBricked)    **Wednesday 11 July**    Participant: Anton, Olle  Experiment: re-streak  39. pSB1K3 - B0034 - SYFP2  88. pSB1C3 - pcat    Participant: Joel Ås  Experiment: screening  ImmR  Pxis    Participant: Hampus, Julia  Experiment: Transformering  pEL3S15 - J23101 - B0034 - amilCP    Experiment: Re-streak  64. pEL3S15 - J23101 - Spot42    Participant: Ellen, Emy  Experiment: Assembly  113. pSB3T5 - J23110 - AAC(6')  53. pSB3K3 - Plac - B0034 - SYFP2    Experiment: Cloning  52. pSB1C3 - TλT1    **Thursday 12 July**    Participant: Hampus, Julia  Experiment: Screening  64. pEL3S15 - J23101 - Spot42  Result: Clone 2 and 7 showed correct band (1500 bp).    Participant: Anton  Experiment: Re-streak  39. pSB1K3 - B0034 - SYFP2    Participant: Johan  Experiment: Screening  pSB1C3 - marR\_BB10  Result: Wrong band.    Participant: Fredrik  Experiment: Ligation  131. pSB1C3 - ImmR  132. pSB1C3 - Pxis    Participant: Olle, Karl  Experiment: Make some beautiful competent cells  Result: Approximate 250 competent cells in our -80 freezer.    Participant: Emy, Ellen  Experiment: Screening  55. pSB3T5 - J23106 - CTX-M  58. pSB3T5 - J23116 - CTX-M  59. pSB3T5 - J23116 - tetA  110. pEL3A15 - PlacQ - CTX-M - lacIs  111. pEL3A15 - PlacQ - tetA - lacIs  115. pSB3T5 - J23110 - tetA  Result: All bad.    Participant: Anna, Fredrik  Experiement: Screening  marR    Experiement: Assembly  112. pSB1C3 - marR    Participant: Joel  Experiment: Transformation  121. pEL3A18 - red  122. pEL3C18 - red  123. pEL3K18 - red    Experiment: PCR purification and digestion  Pxis  ImmR    Participant: Lisa, Sabri  Experiement: Randomized PCR on pEL3S15 - J23101 - Spot42    **Friday 13 July**    Participant: Johan  Experiement: Glycerol stock and plasmid prep.  marR    Participant: Karl  Experiment: Restreak  121. pEL3A18 - red  122. pEL3C18 - red  123. pEL3K18 - red    Participant: Anna, Fredrik  Experiement: Assembly and transformation    93. pSB3C5 - J23110 - tet inv.  94. pSB3C5 - J23106 - tet inv.  95. pSB3C5 - J23101 - tet inv.  96. pSB1C3 - J23106 - B0034  97. pSB1C3 - J23110 - B0034  98. pSB1C3 - J23116 - B0034  99. pSB3T5 - B0034 - SYFP2    Participant: Lisa, Sabri  Experiement: Screening  64. pEL3S15 - J23101 - Spot42    Participant: Olle, Joakim  Experiment: Transformation  52. pSB1C3 - TλT1  53. pSB3T5 - Plac - B0034 - SYFP2  88. pSB1C3 - pCat  113. pSB3T5 - J23110 - AAC(6')  126. pEL3S18 - red  127. pEL3A18 - red(FRT)  128. pEL3C18 - red(FRT)  129. pEL3K18 - red(FRT)  130. pEL3S18 - red(FRT)  123. pEL3A18 - red  124. pEL3C18 - red  125. pEL3K18 - red  131. pSB1C3 - ImmR  132. pSB1C3 - Pxis    Participant: Hampus, Julia, Christoffer  Experiment: Screening, PCR purification, DPNI treatment  specR - J23101  Result: Gave correct bands.    Participant: Anna, Fredrik  Experiment: Nested PCR and screening  OmpF  LexA  Result: LexA good, OmpF has to be redone.    Experiment: Nested PCR  OmpF    Experiment: BioBrick PCR  LexA  OmpF    Participant: Ellen, Emy  Experiment: Screening  55. pSB3T5 - J23106 - CTX-M  58. pSB3T5 - J23116 - CTX-M  59. pSB3T5 - J23116 - tetA  110. pEL3A15 - PlacQ - CTX-M - lacIs  111. pEL3A15 - PlacQ - tetA - lacIs  115. pSB3T5 - J23110 - tetA  Result : 58, 59 wrong, 55 showed no band, 109, 11 and 155 were right.  Experiment: O/N  109. pEL3A15 - PlacIQ - AAC(‘6) - lacIs  111. pEL3A15 -PlacIQ - tetA - lacIs  115. pSB3T5 - J23110 - tetA    **Saturday 14 July**    Participant: Karl  Experiment: Plasmid prep, glycerol stock  39. pSB1K3 - B0034 - SYFP2  109. pEL3A15 - PlacQ - AAC(6') - lacIs  111. pEL3A15 - PlacIQ - AAC(6') - lacIs  115. pSB3T5 - J23110 - tetA    Experiment: O/N from frozen stock  3. pSB3T5 - red    Participant: Ellen  Experiment: Assembly  133. pSB1A3 - J23106 - tetA  134. pSB1A3 - J23116 - tetA  139. pSB3T5 - PT5lac - B0034 - SYFP2  55. pSB3T5 - J23116 - CTX-M  58. pSB3T5 - J23106 - CTX-M    Experiment: Cloning  62. pSB1C3 - AmpR    Participant: Olle  Experiment: Restreak  126. pEL3S18  127. pEL3A18(FRT)  128. pEL3C18(FRT)  129. pEL3K18(FRT)  130. pEL3S18(FRT)  109. pEL3A15 - PlacIQ - AAC(6')  111. pEL3A15 - PlacIQ - tetA  115. pSB3T5 - J23110 - tetA    Experiment: Transformation  113. pSB3T5 - J23110 - AAC(6')  114. pSB3T5 - J23110 - CTX-M    Participant: Joel  Experiment: Cloning  132. pSB1C3 - Pxis    Participant: Anna, Fredrik  Experiment: Assembly and transformation  79. pSB1C3 - J23106 - B0034  80. pSb1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034    Experiment: PCR purification, digestion and ligation into pSB1C3  OmpF  LexA    Experiment: Restreak  93. pSB3C5 - J23110 - tet inv.  94. pSB3C5 - J23106 - tet inv.  95. pSB3C5 - J23101 - tet inv.  96. pSB3T5 - B0034 - SYFP2    Experiment: Screening  OmpF  OmpF (BioBricked)  LexA (BioBricked)    Participant: Emy  Experiment: Restreak  52. pSb1C3 - TλT1  88. pSB1C3 - pCat  131. pSB1C3 - ImmR  60. pSB1A3 - B0032 - SYFP2  157. pSB1C3 - J23101 - B0034    Experiment: O/N  123 pEL3A18 - red  124. pEL3C18 - red  125. pEL3K18 - red    Participant: Arvid  Experiment: Assembly  101. pSB3T5 - J23110 - B0034 - SYFP2    Experiment: Cloning  140. pSB1A5 - B0034 - SYFP2  141. pSB1C5 - B0034 - SYFP2  142. pSB3C3 - B0034 - SYFP2  143. pSB3T3 - B0034 - SYFP2  144. pSB3A15 - B0034 - SYFP2  145. pSB3C15 - B0034 - SYFP2    Participant: Joakim  Experiment: Assembly  53. pSB3T5 - Plac - B0034 - SYFP2    **Sunday 15 July**    Participant: Joel  Experiment: Ligate  132. pSB1C3 - T0    Participant: Ellen  Experiment: Transformation  55. pSB3T5 - J23106 - CTX-M  58. pSB3T5 - J23116 - CTX-M  62. pSB1C3 - AmpR  132. pSB1C3 - Pxis  134. pSB1A3 - J23116 - tetA  139. pSB3T5 - PT5lac - B0034 - SYFP2  147. pSB1C3 - OmpF  148. pSB1C3 - LexA    Participant: Arvid  Experiment: Transformation  100. pSB1AK3 - B1002  101. pSB3T5 - J23110 - B0034 - SYFP2  140. pSB1A5 - B0034 - SYFP2  141. pSB1C5 - B0034 - SYFP2  142. pSB3C3 - B0034 - SYFP2  143. pSB3T3 - B0034 - SYFP2  144. pSB3A15 - B0034 - SYFP2  145. pSB3C15 - B0034 - SYFP2    Experiment: Restreak  53. pSB3T5 - Plac - B0034 - SYFP2    Participant: Anna, Fredrik  Experiment:  93. pSB3C5 - J23110 - tet inv.  94. pSB3C5 - J23106 - tet inv.  95. pSB3C5 - J23101 - tet inv  96. pSB3T5 - B0034 - SYFP3    Participant: Fredrik  Experiment: Assembly  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034    Participant: Caroline, Emy  Experiment: Screening  52. pSB1C3 - TλT1  131. pSB1C3 - ImmR    Experiment: O/N  123. pEL3A18 - red  124. pEL3K18 - red  131. pSB1C3 - ImmR    Participant: Hampus, Olle  Experiment: Mutagenesis - insertion of random nucleotides into pEL3S15 - J23101 - Spot42    Experiment: Screening  111. pEL3A15 - PlacIQ - tetA  115. pSB3T5 - J23110 - tetA    Participants: Olle  Experiment: Restreak  109. pEL3A15 - PlacIQ - AAC(6')  113. pSB3T5 - J23110 - AAC(6')  114. pSB3T5 - J23110 - CTX-M    Participant: Joel, Tobias  Experiment: Assembly  133. pSB1A3 - J23106 - tetA  134. pSB1A3 - J23116 - tetA    Experiment: Transformation  116. pEL3C18 - J23116 - AAC(6') - lacIs  117. pEL3A18 - PlacIQ - AAC(6') - SYFP2  118. pEL3A18 - PlacIQ - CTX-M - SYFP2  119. pEL3A18 - PlacIQ - tetA - SYFP2  120. pEL3A18 - J23106 - tetA - SYFP2  121. pEL3A18 - J23106 - CTX-M - SYFP2  122. pEL3C18 - J23106 - AAC(6') - lacIs  123. pEL3C18 - J23106 - tetA - lacIs  136. pEL3C18 - PlacIQ - AAC(6') - lacIs  137. pEL3C18 - PlacIQ - tetA - lacIs  138. pEL3C18 - PlacIq - CTX-M - lacIs    VECKA 5    **Monday 16 July**    Participant: Sabri, Lisa  Experiment: Mutagenesis - sRNA library  Result: No band on gel.    Participant: Caroline, Katarina  Experiment: Restreak  52. TλT1  55. pSB3T5 - J23106 - CTX-M  58. pSB3T5 - J23116 - CTX-M  133. pSB1A3 - J23106 - tetA  134. pSB1A3 - J23116 - tetA    Experiment: O/N  52. TλT1  113. pSB3T5 - J23110 - AAC(6')  114. pSB3T5 - J23110 - CTX-M    Participant: Olle, Arvid  Experiment: Restreak  140. pSB1A5 - B0034 - SYFP2  143. pSB3T5 - B0034 - SYFP2  144. pEL3A15 - B0034 - SYFP2  145. pEL3C15 - B0034 - SYFP2    Participant: Anton  Experiment: Assembly  101. pSB3T5 - J23110 - B0034 - SYFP2    Experiment: Prepare for sequencing  39.1,2 pSB1K3 - B0034 - SYFP2  93.2,4 pSB3C5 - J23110 - tet inv.  95.4 pSB3C5 - J23101 - tet inv.  115.2,3 pSB3T5 - J23110 - tetA  131.3,4 pSB1C3 - ImmR    Participant: Karl  Experiment: Restreak  147. pSB1C3 - LexA  148. pSB1C3 - OmpF    Participant: Julia  Experiment: O/N  96. pSB3T5 - B0034 - SYFP2    Participant: Julia, Hampus  Experiment: Restreak  MG1655 - SpecR - J23101    Participant: Olle  Experiment: Screening  52. TλT1  113. pSB3T5 - J23110 - AAC(6')  114. pSB3T5 - J23110 - CTX-M  14. pSB4K5 - lacIs  109. pEL3A15 - PlacIQ - AAC(‘6) - lacIs  Result: All good.    Participant: Joel, Joakim    Experiment: Plasmid prep.  131. pSb1C3 - ImmR  115. pSB3T5 - J23110 - tetA    Participant: Anna, Anders  Experiment: Plasmid prep.  93. pSB3C5 - J23110 - tet inv.  95. pSb3C5 - J23101 - tet inv.  96. pSB3T5 - B0034 - SYFP2  9. pSB1C3 - CTX-M  124. pEL3C18 - red    Participant: Karl  Experiment: Transformation  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034  101. pSB1T5 - J23110 - B0034 - SYFP2    Participant: Julia, Hampus  Experiment: Plasmid prep. and glycerol stock  123. pEL3A18 - red  125. pEL3K18 - red  126. pEL3S18 - red  127. pEL3A18(FRT) - red  128. pEL3C18(FRT) - red  129. pEL3K18(FRT) - red  130. pEL3S18(FRT) - red    Participant: Arvid  Experiment: Restreak  53. pSB3T5 - Plac - B0034 - SYFP2  100. pSB1AK3 - B1002    **Tuesday 17 July**    Participant:Lisa, Sabri  Experiment: Mutagenesis and screening of randomized sRNA library  Result: No visible bands on gel.    Participant: Hampus, Julia  Experiment: Screening  MG1655 - SpecR - J23101 - Cam  MG1655 - SpecR - J23101 - Tet  134. pSB1A3 - J23116 - tetA  133. pSB1A3 - J23106 - tetA  Result: 133, 134 correct length. Preparation of the other two was done incorrectly, no bands.    Participant: Johan, Anders  Experiment: Screening  52. TλT1  55. J23106 - CTX-M  14. lacIs  112. pSB1C3 - marR  Result: Screening has to be redone.    Participant: Caroline, Katarina  Experiment: Transformation  116. pEL3C18 - J23116 - AAC(6') - lacIs  117. pEL3A18 - PlacIQ - AAC(6') - SYFP2  118. pEL3A18 - PlacIQ - CTX-M - SYFP2  119. pEL3A18 - PlacIQ - tetA - SYFP2  120. pEL3A18 - J23106 - AAC(6') - SYFP2  121. pEL3A18 - J23106 - tetA - SYFP2  133. pSB1A3 - J23106 - AAC  134. pSB1A3 - J23116 - tetA  136. pEL3C18 - PlacIQ - AAC(6') - lacIs  137. pEL3C18 - PlacIQ - CTX-M - lacIs  138. pEL3C18 - PlacIQ - tetA - lacIs  146. pSB1C3 - T0  151. pEL3C18 - J23106 - AAC(6') - lacIs  152. pEL3C18 - J23106 - tetA - lacIs    Experiment: Assembly  62. pSB1C3 - AmpR  132. pSB1C3 - Pxis  139. pSB3T5 - T5lac - B0034 - SYFP2    Participant: Olle  Experiment: Screening  53. pSB3K3 - Plac - B0034 - SYFP2  100. pSB1AK3 - B1002    Participant: Karl  Experiment: Restreak  101. pSB3T5 - J23110 - B0034 - SYFP2    Participant: Anna  Experiment: Screening  147. pSB1C3 - LexA  148. pSB1C3 - OmpF  Result: OmpF might be good, LexA will be redone.    Participant: Anton  Experiment: Plasmid prep.  55. pSB3T5 - J23106 - CTX-M  Result: Too low concentration to use.    Experiment: Preparation for sequencing  52. pSB1C3 - TλT1  113. pSB3T5 - J23110 - AAC(6')  114. pSB3T5 - J23110 - CTX-M    Participant: Joakim    Experiment: Plasmid prep.  52. pSB1C3 - TλT1  53. pSB3K3 - Plac - B0034 - SYFP2  113. pSB3T5 - J23110 - AAC(6')  114. pSB3T5 - J23110 - CTX-M    **Wednesday 18 July**    Participant: Arvid, Anton  Experiment: Test if chloramphenicol can increase plasmid yield, O/N of:  140. pSB1A5 - B0034 - SYFP2  143. pSB3T5 - B0034 - SYFP2  with and without addition of chloramphenicol two hours before plasmid preparation.  Result: Slight increase, less than expected.    Participant: Emy, Caroline, Katarina, Joel  Experiment: Screening  55. pSB3T5 - J23106 - CTX-M  58. pSB3T5 - J23116 - CTX-M  133. pSB1A3 - J23106 - tetA  134. pSB1A3 - J23116 - tetA  lacIs PCR product  Result: 134 was wrong, the others had clones with inserts of correct lengths.    Participant: Anton  Experiment: Plasmid prep.  52. pSB1C3 - TλT1  55. pSB3T5 - J23106 - CTX-M  100. pSB1AK3 - B1002  148. pSB1C3 - OmpF    Participant:Lisa, Sabri  Experiment: Mutagenesis PCR of randomized sRNA library  Result: Failed!    Participant: Julia, Hampus  Experiment: Screening  MG1655 - SpecR - J23101 - Cam  MG1655 - SpecR - J23101 - Tet  Result: Both were of the right length.    Experiment: O/N  101. pSB3T5 - J23110 - B0034 - SYFP2  148. pSB1C3 - OmpF  149. MG1655 - SpecR - J23101 - Tet  150. MG1655 - SpecR - J23101 - Cam    Participant: Anna  Experiment: Cloning and transformation  156. pJN3C1 - AmpR      Participant: Anna, Joakim  Experiment: Transformation  62. pSB1C3 - AmpR  94. pSB3C5 - J23106 - tet inv.  132. pSB1C3 - Pxis  139. pSB3T5 - PT5lac - B0034 - SYFP2  147. pSB1C3 - LexA  lacI (from kit)    **Thurusday 19 July**    Participant: Caroline, Katarina, Emy  Experiment: Screening  53. pSB3T5 - Plac - B0034 - SYFP2  118. pEL3A18 - PlacIQ - CTX-M - SYFP2  119. pEL3A18 - PlacIQ - tetA - SYFP2  121. pEL3A18 - J23106 - tetA - SYFP2  134. pSB1A3 - J23116 - tetA  146. pSB1C3 - T0    Experiment: O/N  53. pSB3T5 - Plac - B0034 - SYFP2  58. pSB3T5 - J23116 - CTX-M  119. pEL3A18 - PlacIQ - tetA - SYFP2  146. pSB1C3 - T0    Experiment: Restreak  118. pEL3A18 - PlacIQ - CTX-M - SYFP2    Participant: Sabri, Lisa  Experiment: Mutagenesis of randomized sRNA library. DMSO added to prevent primers from folding secondary structures.    Participant: Joakim  Experiment: Restreak  94. pSB3C5 - J23106 - tet inv.  139. pSB3T5 - Pt5lac - SYFP2  155. pSB1A3 - lacI    Participant: Anna  Experiment: Screening  148. pSB1C3 - OmpF  11. pSB1C3 - SYFP2 and 24. pSB2K3 - tet inv. to see if they have been mixed    Experiment: Assembly  132. pSB1C3 - Pxis  156. pJN3C1 - AmpR    Participant: Joel  Experiment: Screening  55. pSB3T5 - J23116 - CTX-M  14. lacIs (PCR product)  Result: 55 wrong, lacIs correct length.    Participant: Arvid, Anton  Experiment: Screening, plasmid prep. and glycerol stock  101. pSB3T5 - J23110 - B0034 - SYFP2    Experiment: Transformation  158. pSB4K5 - red    Participant: Julia  Experiment: PCR purification  149. MG1655 - SpecR - J23101 - Tet    Participant: Anders, Johan  Experiment: Screening  116. pEL3C18 - J23116 - AAC(6') - lacIs  117. pEL3A18 - PlacIQ - AAC(6') - SYFP2  120. pEL3A18 - AAC(6') - SYFP2  136. pEL3C18 - PlacIQ - AAC(6') - lacIs  146. pSB1C3 - T0  151. pEL3C18 - J23106 - AAC(6') - lacIs  Result: Unknown.    **Friday 20 July**    Participant: Emy, Katarina, Caroline, Joel  Experiment: Screening  116. pEL3C18 - J23116 - AAC(6') - lacIs  117. pEL3A18 - PlacIQ - AAC(6') - SYFP2  120. pEL3A18 - AAC(6') - SYFP2  136. pEL3C18 - PlacIQ - AAC(6') - lacIs  146. pSB1C3 - T0  151. pEL3C18 - J23106 - AAC(6') - lacIs  Result: 116, 120, 136 good!    Experiment: Assembly  55. pSB3Tt5 - J23106 - CTX-M  121. pEL3A18 - J23106 - tetA - SYFP2  134. pSB1A3 - J23116 - tetA    Experiment: Screening  53. pSB3K3 - Plac - B0034 - SYFP2  58. pSB3Tt5 - J23116 - CTX-M  119. pEL3A18 - PlacIQ - tetA - SYFP2  Result: 53 and 58 had the right length.    Experiment: Plasmid prep.  53. pSB3K3 - Plac - B0034 - SYFP2  58. pSB3T5 - J23116 - CTX-M  119. pEL3A18 - PlacIQ - tetA - SYFP2    Participant: Joakim, Anna  Experiment: Screening  70. pSB3C5 - J23106 - tet inv.  139. pSB3T5 - Pt5lac - B0034 - SYFP2  147. pSB1C3 - LexA  155. pSB1A3 - lacI  Result: RESULTAT RESULTAT RESULTAT RESULTAT    Participant: Joel  Experiment: PCR amplification of lacIs with RBS and promoter removed. Screened on  gel.  Result: PCR failed.    Participant: Sabri, Lisa  Experiment: Screening  pEL3S15 - J23101 - random sRNA - Spot42  Result: 10% DMSO showed a band on the gel. New PCR with higher concentrations of  DMSO will be made.    Participant: Anna  Experiment: Transformation  55. pSB3Tt5 - J23106 - CTX-M  115. pSB3T5 - J23110 - tetA  121. pEL3A18 - J23106 - tetA - SYFP2  134. pSB1A3 - J23116 - tetA    Participant: Anders, Johan  Experiment: Screening  101. pSB3T5 - J23110 - B0034 - SYFP2  116. pEL3C18 - J23116 - AAC(6') - lacIs  117. pEL3A18 - PlacIQ - AAC(6') - SYFP2  120. pEL3A18 - AAC(6') - SYFP2  136. pEL3C18 - PlacIQ - AAC(6') - lacIs  146. pSB1C3 - T0  151. pEL3C18 - J23106 - AAC(6') - lacIs  Result: Gels failed. T0 seemed right.    Participant: Julia  Experiment: Restreak  137. pEL3C18 - PlacIQ - tetA - lacIs  138. pEL3C18 - PlacIQ - CTX-M - lacIs  152. pEL3C18 - J23106 - tetA - lacIs    Experiment: Glycerol stock  149. MG1655 - SpecR - J23101 - Tet    **Saturday 21 July**    Participant: Caroline, Katarina, Emy  Experiment: Screening  116. pEL3C18 - J23116 - AAC(6') - lacIs  117. pEL3A18 - PlacIQ - AAC(6') - SYFP2  120. pEL3A18 - AAC(6') - SYFP2  136. pEL3C18 - PlacIQ - AAC(6') - lacIs  146. pSB1C3 - T0  151. pEL3C18 - J23106 - AAC(6') - lacIs  Result: 116, 120 and 136 ok, the others were wrong.    Experiment: O/N  116. pEL3C18 - J23116 - AAC(6') - lacIs  120. pEL3A18 - AAC(6') - SYFP2  136. pEL3C18 - PlacIQ - AAC(6') - lacIs    Experiment: Assembly  117. pEL3A18 - PlacIQ - AAC(6') - SYFP2  118. pEL3A18 - PlacIQ - CTX-M - SYFP2    Experiment: Restreak  119. pEL3A18 - PlacIQ - tetA - SYFP2  137. pEL3C18 - PlacIQ  146. pSB1C3 - T0  152. pEL3C18 - J23106 - tetA - lacIs    Participant: Julia  Experiment: Screening  137. pEL3C18 - PlacIQ - CTX-M - lacIs  138. pEL3C18 - J23106 - lacIs  152. pEL3C18 - PlacIQ - tetA - lacIs  Result: 137 and 152 good.    Experiment: Restreak  137. pEL3C18 - PlacIQ - CTX-M - lacIs  152. pEL3C18 - PlacIQ - tetA - lacIs    **Sunday 22 July**    Participant: Anna  Experiment: Screening  139. pSB3T5 - PT5lac - B0034 - SYFP2  147. pSB1C3 - LexA    Participant: Joel, Emy, Caroline, Katarina, Karl, Pikkei  Experiment: Screen  115. pSB3T5 - J23110 - tetA  119. pEL3A18 - PlacIQ - tetA - SYFP2    **Monday 23 July**    Participant: Tobias, Christoffer  Experiment: PCR cloning of cat-sacB with λ primers for chromosomal integration    Participant: Sabri, Lisa  Experiment: Mutagenesis, with different concentrations of DMSO  pEL3S15 - J23101 - random sRNA - Spot42    Experiment: Screening  pEL3S15 - J23101 - random sRNA - Spot42  Result: Gel failed!    Participant: Olle  Experiment: Screening  159. pSB1C3 - pLlacO - B0034  160. pSB1C3 - T5lac - B0034  161. pSB1C3 - PrecA - B0034 - SYFP2  Result: All ok!    Participant: Joel, Ellen, Karl  Experiment: Phosphorylation of primers  lacIs\_linker\_CTX\_F  B0034(mod)\_R    Experiment: Mutagenesis  lacIs    Experiment: Plasmid prep.  115. pSB3T5 - J23110 - tetA  119. pEL3A18 - PlacIQ - tetA - SYFP2  121. pEL3A18 - J23106 - tetA - SYFP2  132. pSB1C3 - Pxis    Experiment: O/N  53. pSB3K3 - Plac - B0034 - SYFP2  58. pSB3T5 - J23116 - CTX-M  115. pSB3T5 - J23110 - tetA  119. pEL3A18 - PlacIQ - tetA - SYFP2  146. pSB1C3 - T0  158. pSB4K5 - red    Participant: Fredrik  Experiment: Plasmid prep.  55. pSB3T5 - J23106 - CTX-M  139. pSB3T5 - PT5lac - B0034 - SYFP2  147. pSB1C3 - LexA    Participant: Joakim, Anna  Experiment: Screening  80. pSB1C3 - J23110 - B0034  Result: OK band present on gel    Participant: Julia, Hampus  Experiment: Assembly  114. pSB1K3 - Pxis - B0034 -SYFP2  115. pEL3S15 - ImmR - λT1    Participant: Anton  Experiment: Preparation for sequencing  53. pSB3K3 - Plac - B0034 - SYFP2  55. pSB3T5 - J23106 - CTX-M  116. pEL3C18 - J23116 - AAC(6') - lacIs  120. pEL3A18 - J23106 - AAC(6') - SYFP2  136. pEL3C18 - PlacIQ - AAC(6') - lacIs  147. pSB1C3 - LexA  148. pSB1C3 - OmpF  149. MG1655 - SpecR - J23101    Participant: Arvid, Karl  Experiment: Screening  101. PSB3T5 - J23110 - B0034 - SYFP2  Result: All clones good!    Participant: Karl  Experiment: Assembly  134. pSB1A3 - J23116 - tetA    Experiment: Transformation  134. pSB1A3 - J23116 - tetA  162. pEL3A18 - J23110 - AAC(6') - SYFP2  163. pEL3A18 - J23110 - CTX-M - SYFP2    Experiment: Screening  60. pSB1A2 - B0032 - SYFP2  Result: Three out of six clones had the right length    **Tuesday 24 July**    Participant: Caroline, Katarina, Emy  Experiment: Screening  117. pEL3A18 - PlacIQ - AAC(6') - SYFP2  118. pEL3A18 - PlacIQ - CTX-M - SYFP2  Result: Both had clones with correct lengths    Experiment: Assembly and transformation  166. pEL3C18 - PlacIQ - AAC(6') - lacI  167. pEL3C18 - PlacIQ - CTX-M - lacI  168. pEL3C18 - PlacIQ - tetA - lacI  169. pEL3C18 - J23106 - AAC(6') - lacI  170. pEL3C18 - J23106 - tetA - lacI    Experiment: Transformation  pBad  AmpR  33. pSB3T5 - PT5lac - B0034  101. pSB3C5 - J23110 - B0034 - SYFP2  172. pSB3C5 - tet inv. - B0034 - SYFP2    Participant: Joel, Hampus, Julia  Experiment: Plasmid prep.  53. pSB3K3 - Plac - B0034 - SYFP2  58. pSB3T5 - J23116 - CTX-M  119. pEL3A18 - PlacIQ - tetA - SYFP2  Result: Concentration on 58 was too low for sequencing    Participant: Anton, Joakim  Experiment: Preparation for sequencing  53. pSB3K3 - Plac - B0034 - SYFP2  55. pSB3T5 - J23106 - CTX-M  101. pSB3C5 - J23110 - B0034 - SYFP2  116. pEL3C18 - J23116 - AAC(6') - lacIs  120. pEL3A18 - J23106 - AAC(6') - SYFP2  132. pSB1C3 - Pxis  136. pEL3C18 - PlacIQ - AAC(6') - lacIs  139. pSB3T5 - PT5lac - B0034 - SYFP2  147. pSB1C3 - LexA  149. MG1655 - SpecR - J23101  159. pSB1C3 - pLlacO - B0034  160. pSB1C3 - T5lac - B0034  161. pSB1C3 - PrecA - B0034 - SYFP2    Participant: Sabri, Lisa  Experiment: Screening, phosphorylation of primers, PCR purification, DPN1 treatment  pEL3S15 - J23101 - random sRNA - Spot42  Result: 10% and 14% DMSO showed bands on the gel    Participant: Fredrik  Experiment: Plasmid prep.  115. pSB3T5 - J23110 - tetA  146. pSB1C3 - T0    Participant: Tobias, Christoffer  Experiment: Screening  cat-sacB  Result: Contamination, no clear bands    Participant: Johan  Experiment: Assembly  33. pSB1A3 - PT5lac - B0034  102. pSB3K5 - J23110 - B0034 - SYFP2 - B1002  172. pSB3C5 - tet inv. - B0034 - SYFP2    Participant: Karl  Experiment: Cloning  97. pSB3T5 - pBad  156. pJN3C1 - AmpR    Participant: Olle  Experiment: Glycerol stock and plasmid prep.  159. pSB1C3 - PLlacO - B0034  160. pSB1C3 - T5lac - B0034  161. pSB1C3 - PrecA - B0034 - SYFP2    Participant: Julia, Hampus  Experiment: Transformation  164. pEL3S15 - Pxis - B0034 - SYFP2  165. ImmR - TλT1    Experiment: O/N  52. pSB1C3 - TλT1    Participant: Joakim, Fredrik  Experiment: Phosphorylation of primers  LexA(D85G)\_R  LexA(D85G)\_F  marR(mut)\_R  marR(mut)\_F    **Wednesday 25 July**    Participant: Emy, Caroline, Katarina, Ellen  Experiment: Screening  118. pEL3A18 - PlacIQ - CTX-M - SYFP2  134. pSB1A3 - J23116 - tetA  162. pEL3A18 - J23110 - AAC(6') - SYFP2  163. pEL3A18 - J23110 - CTX-M - SYFP2  Result: 134 good, 118 and 162 have to be redone. 163 will not be continued.    Participant: Hampus, Julia  Experiment: PCR purification, ligation  pEL3S15 - J23101 - random sRNA - Spot42    Experiment: Assembly  164. pEL3S15 - Pxis - B0034 - SYFP2  165. pSB1K3 - ImmR - TλT1    Participant: Karl  Experiment: Restreak  97. pSB3T5 - pBad  156. pJN3C1 - AmpR    Experiment: New cloning and transformation, in case the previous one failed  97. pSB3T5 - pBad    Experiment: Transformation  14. pSB4K5 - lacIs    Experiment: O/N  158. pSB4k5 - red    Participant: Olle  Experiment: Restreak  33. pSB3T5 - PT5lac - B0034  102. pSB3C5 - J23110 - B0034 - SYFP2 - B1002  172. pSB3C5 - tet inv. B0034 - SYFP2    Participant: Tobias, Christoffer  Experiment: PCR cloning and screening  84. pSB3T5 - cat-sacB  Result: Bad gel.    Participant: Anton  Experiment: Preparation for sequencing  60. pSB1A3 - B0032 - SYFP2  79. pSB1C3 - J23106 - B0034  112. pSB1C3 - marR  146. pSB1C3 - T0    Participant: Julia  Experiment: Plasmid prep.  52. pSb1C3 - TλT1    Participant: Arvid, Anton  Experiment: Screening, preparation for sequencing  60. pSB1A3 - B0032 - SYFP2    **Thursday 26 July**    Participant: Joel  Experiment: Glycerol stock and plasmid prep.  60. pSB1A3 - B0034 - SYFP2  134. pSB1A3 - J23116 - tetA    Participant: Christoffer, Tobias  Experiment: PCR cloning and screening  84. pSB3T5 - cat-sacB  Result: Good bands.    Participant: Caroline, Emy, Katarina  Experiment: Screening  118. pEL3A18 - PlacIQ - CTX-M - SYFP2  162. pEL3A18 - J23110 - AAC(6') - SYFP2  166. pEL3C18 - PlacIQ - AAC(6') - lacI  167. pEL3C18 - PlacIQ - CTX-M - lacI  168. pEL3C18 - PlacIQ - tetA - lacI  169. pEL3C18 - J23106 - AAC(6') - lacI  170. pEL3C18 - J23106 - tetA - lacI  Result: 118 and 162 had good clones.    Experiment: Restreak  117. pEL3A18 - PlacIQ - AAC(6') - SYFP2    Experiment: O/N  118. pEL3A18 - PlacIQ - CTX-M - SYFP2  162. pEL3A18 - J23110 - AAC(6') - SYFP2    Participant: Hampus, Julia  Experiment: Screening  164. pEL3S15 - Pxis - B0034 - SYFP2  165. pSB1K3 - ImmR - TλT1  Result: Both had clones with the right lengths.    Experiment: O/N  156. pJN3C1 - AmpR  164. pEL3S15 - Pxis - B0034 - SYFP2  165. pSB1K3 - ImmR - TλT1    Participant: Karl  Experiment: Screening  97. pSB3T5 - pBad  172. pSB3C5 - tet inv. - B0034 - SYFP2  Result: 172 was good, 97 was not.    Participant: Olle  Experiment: Restreak  33. pSB3T5 - PT5lac - B0034  102. pSB3C5 - J23110 - B0034 - SYFP2 - B1002    Experiment: O/N  158. pSB3K5 - red    **Friday 27 July**    Participant: Tobias, Christoffer  Experiment: Assembly  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034    Experiment: DPN1 treatment  84. pSB3T5 - cat-sacB     Participant: Caroline, Emy, Katarina, Joel  Experiment: Screening and O/N  117. pEL3A18 - PlacIQ - AAC(6') - SYFP2  Result: Good!    Experiment: Screening  167. pEL3C18 - PlacIQ - PlacIQ - CTX-M - lacI  Result: Bad! Turns out lacI was something completely different, it had been taken from the wrong well in the kit.    Experiment: Assembly  166. pEL3C18 - PlacIQ - AAC(6') - lacI  167. pEL3C18 - PlacIQ - CTX-M - lacI  168. pEL3C18 - PlacIQ - tetA - lacI  169. pEL3C18 - J23106 - AAC(6') - lacI    Experiment: Streak from frozen stock  37. pSB3T5 - PlacIQ - CTX-M  38. pSB3T5 - PlacIQ - tetA  56. pSB3T5 - J23106 - tetA    Participant: Ellen  Experiment: Assembly  173. pEL3C18 - J23106 - CTX-M - SYFP2  174. pEL3C18 - J23106 - CTX-M - lacI    Experiment: Transformation  173. pEL3C18 - J23106 - CTX-M - SYFP2  174. pEL3C18 - J23106 - CTX-M - lacI  166. pEL3C18 - PlacIQ - AAC(6') - lacI  167. pEL3C18 - PlacIQ - CTX-M - lacI  168. pEL3C18 - PlacIQ - tetA - lacI  169. pEL3C18 - J23106 - AAC(6') - lacI    Participant: Olle, Karl  Experiment: Screening  33. pSB3T5 - PT5lac - B0034  97. pSB3T5 - pBad  102. pSB3C5 - J23110 - B0034 - SYFP2 - B1002  Result: All wrong.    Participant: Hampus  Experiment: Assembly and transformation  139. pSB3T5 - PT5lac - B0034 - SYFP2  177. pSB3T5 - AmpR - T0    Experiment: Glycerol stock and plasmid prep.  156. pJN3C1 - AmpR  164. pEL3S15 - Pxis - B0034 - SYFP2  165. pSB1K3 - ImmR - TλT1    Participant: Olle  Experiment: Restreak  101. pSB3T5 - J23110 - B0034 - SYFP2    Participant: Arvid  Experiment: Glycerol stock and plasmid prep.  118. pEL3A18 - PlacIQ - CTX-M - SYFP2  158. pSB4K5 - red  162. pEL3A18 - J23110 - AAC(6') - SYFP2  172. pSB3C5 - tet inv. - B0034 - SYFP2    **Saturday 28 July**    Participant: Tobias, Christoffer  Experiment: Transformation  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034    Participant: Arvid, Karl  Experiment: Screening  33. pSB3T5 - PT5lac - B0034  97. pSB3T5 - pBad  101. pSB3T5 - J23110 - B0034 - SYFP2  158. pSB4K5 - red  172. pSB3C5 - tet inv. - B0034 - SYFP2  Result: 33 and 97 wrong, 101 contaminated and has to be screened again, 158 and 172 seem OK.    Participant: Caroline, Emy, Katarina  Experiment: Phosphorylation of primers for and mutagenesis of:  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs  180. pEL3C18 - J23106 - AAC(6') - linker - SYFP2    Participant: Joel  Experiment: Assembly and transformation  137. pEL3C18 - PlacIQ - CTX-M - lacIs  138. pEL3C18 - PlacIQ - tetA - lacIs  151. pEL3C18 - J23106 - AAC(6') - lacIs  182. pEL3C18 - J23106 - CTX-M - lacIs  183. pSB1C3 - Ptac    Participant: Karl  Experiment: O/N  37. pSB3T5 - PlacIQ - CTX-M  38. pSB3T5 - PlacIQ - tetA  56. pSB3T5 - J23106 - tetA  101. pSB3T5 - J23116 - B0034 - SYFP2 - B1002  155. pSB1A2 - lacI    Participant: Fredrik  Experiment: Assembly  175. pSB1K3 - PLlacO - B0034  176. pSB1K3 - PT5lacO - B0034    Participant: Ellen  Experiment: Glycerol stock and plasmid prep.  33. pSB3T5 - PT5lac - B0034  117. pEL3A18 - PlacIQ - AAC(6') - SYFP2    Participant: Hampus  Experiment: Restreak  139. pSB3T5 - PT5lac - B0034 - SYFP2  177. pSB3T5 - AmpR - T0    **Sunday 29 July**    Participant: Hampus  Experiment: Ligation  92. pEL3S15 - J23101 - random sRNA - Spot42    Participant: Tobias, Christoffer  Experiment: Restreak  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034    Participant: Karl  Experiment: Streak from frozen stock  60. pSB1A3 - B0032 - SYFP2    Participant: Olle  Experiment: Screening  101. pSB3T5 - J23110 - B0034 - SYFP2  Result: Possibly one good clone    Participant: Fredrik, Pikkei  Experiment: Mutagenesis PCR  112. pSB1C3 - MarR  147. pSB1C3 - LexA  148. pSB1C3 - OmpF  Result: All had clones of the correct length.    Participant: Caroline, Katarina, Emy, Joel  Experiment: Mutagenesis, transformation  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs  180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2    Experiment: Assembly and transformation  152. pEL3C18 - J23106 - tetA - lacIs  181. pEL3A18 - J23106 - CTX-M - SYFP2  Result: All clones of 152 were red, assembly will be redone.    Experiment: Restreak  137. pEL3C18 - PlacIQ - CTX-M - lacIs  138. pEL3C18 - PlacIQ - tetA - lacIs  151. pEL3C18 - J23106 - AAC(6') - lacIs  182. pEL3C18 - J23106 - CTX-M - lacIs  183. pSB1C3 - Ptac    Participant: Hampus  Experiment: Restreak  139. pSB3T5 - PT5lac - B0034 - SYFP2  177. pSB3T5 - AmpR - TT0    Participant: Ellen  Experiment: Glycerol stock and plasmid prep.  37. pSB3T5 -PlacIQ - CTX-M  38. pSB3T5 - PlacIQ - tetA  56. pSB3T5 - J23106 - tetA  101. pSB3T5 - J23110 - B0034 - SYFP2 - B1002  155. pSB1A2 - lacI    Experiment: Streak from frozen stock  56. pSB3T5 - J23106 - tetA    **Monday 30 July**    Participant: Julia, Hampus  Experiment: Screening  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034  139. pSB3T5 - PT5lac - B0034 - SYFP2  177. pSB3T5 - AmpR - TT0  183. pSB1C3 - Ptac  Result: 79, 80, 81, 183 gave good bands. 139 and 177 showed the wrong bands.    Experiment: O/N  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034  183. pSB1C3 - Ptac    Participant: Anton  Experiment: Assembly and transformation  184. pEL3S15 - AmpR - T0    Participant: Tobias, Christoffer  Experiment: Insertion of cat-sacB onto the chromosome of MG1655-J23101 with λ-red    Participant: Caroline, Emy, Katarina  Experiment: Screening  137. pEL3C18 - PlacIQ - CTX-M - lacIs  138. pEL3C18 - PlacIQ - tetA - lacIs  151. pEL3C18 - J23106 - AAC(6') - lacIs  182. pEL3C18 - J23106 - CTX-M - lacIs  Result: 151 and 182 OK, 137 and 138 failed.    Experiment: O/N  56. pSB3T5 - J23106 - tetA  60. pSB1A3 - B0032 - SYFP2  151. pEL3C18 - J23106 - AAC(6') - lacIs  182. pEL3C18 - J23106 - CTX-M - lacIs    Experiment: Restreak  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs  180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2  181. pEL3A18 - J23106 - CTX-M - SYFP2    Participant: Olle  Experiment: Phosphorylation of primers  pUC\_BB\_F  pUC\_BB\_R  pEL8to4C15\_F  pEL8to4C15\_R    Experiment: Phusion PCR to BioBrick pUC ORI from pSB1A3    Participant: Fredrik  Experiment: PCR purification, ligation and transformation  112. pSB1C3 - MarR  147. pSB1C3 - LexA  148. pSB1C3 - OmpF    Experiment: Screening  175. pSB1K3 - PLlacO - B0034  Result: All clones wrong.    **Tuesday 31 July**    Participant: Arvid  Experiment: Prepare agar stabs for sending to other teams  pSB1A3 - amilCP  pSB1C3 - amilGFP  pSB1C3 - FixJ  pSB1C3 - YF1  pSB1C3 - B0034 - YF1 - B0034 - FixJ  pSB1C3 - PfixK2    Participant: Joakim  Experiment: Restreak  184. pEL3S15 - AmpR - T0    Participant: Karl  Experiment: O/N  12. pSB1A2 - B0034    Experiment: PCR purification  BioBricked pUC-ORI  182. pEL3C18 - J23106 - CTX-M - lacIs    Experiment: Mutagenesis PCR of pEL8C15 to pEL4C15    Experiment: Screening  pUC-ORI  pEL4C15  Result: Both seem OK.    Participant: Emy, Caroline, Katarina, Ellen  Experiment: Screening  137. pEL3C18 - PlacIQ - CTX-M - lacIs  138. pEL3C18 - PlacIQ - tetA - lacIs  181. pEL3A18 - J23106 - CTX-M - SYFP2  Result: 137 and 138 have clones of the correct lengths. 181 was all wrong.    Experiment: Assembly and transformation  152. pEL3C18 - J23106 - tetA  181. pEL3A18 - J23106 - CTX-M - SYFP2    Participant: Joel, Karl  Experiment: Screening  14. pSB4K5 - lacIs  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  179. pEL3C18 - PlacIQ - AAC(6´) - linker - lacIs  180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2   Result: All seem to have good clones.    Experiment: O/N  137. pEL3C18 - PlacIQ - CTX-M - lacIs  138. pEL3C18 - PlacIQ - tetA - lacIs    Participant: Olle  Experiment: Digestion  BioBricked pUC-ORI    Participant: Hampus, Julia  Experiment: Screening and O/N  139. pSB3T5 - PT5lac - B0034 - SYFP2  177. pSB3T5 - AmpR - TT0  Result: Possible good clones of both.    Experiment: Assembly  186. pJN3K1 - AmpR - TT0    Participant: Fredrik  Experiment: Plasmid prep.  1. pSB1C3 - red  12. pSB1A2 - B0034  86. pSB1A2 - PLlacO    Experiment: Assembly  175. pSB1K3 - PLlacO - B0034    Participant: Julia  Experiment: Plasmid prep.  60. pSB1A3 - B0032 - SYFP2  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034  183. pSB1C3 - Ptac    Participant: Christoffer, Tobias  Experiment: Streak MG1655 - J23101 - cat-sacB on plates with chloramphenicol and sucrose.    Experiment: NotI treatment  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034  183. pSB1C3 - Ptac    Participant: Anton, Olle  Experiment: Plasmid prep.  56. pSB3T5 - J23106 - tetA  74. pEL3C15 - PlacIQ - CTX-M - lacIs - TλT1  80. pSB1C3 - J23110 - B0034    Experiment: Preparation for sequencing  151. pEL3C18 - J23106 - AAC(6') - lacIs  182. pEL3C18 - J23106 - CTX-M - lacIs  183. pSB1C3 - Ptac    **Wednesday 1 August**    Participant: Caroline, Emy, Katarina  Experiment: Screening  138. pEL3C18 - tetA - lacIs  Result: One good clone!    Experiment: Glycerol stock and plasmid prep.  post-mutagenesis pSB4K5 - lacIs  137. pEL3C18 - PLlacO - CTX-M - lacIs  138. pEL3C18 - tetA - lacIs  179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs  180. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2    Experiment: Restreak  152. pEL3A18 - J23106 - tetA - lacIs  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2     Participant: Joel  Experiment: Plasmid prep.  12. pSB1A2 - B0034    Participant: Fredrik  Experiment: Screening and O/N  190. pSB1C3 - MarR(mut)  191. pSB1C3 - LexA(G85D)  192. pSB1C3 - OmpF  Result: All had good clones!    Experiment: Transformation  175. pSB1C3 - PLlacO - B0034    Participant: Joakim, Johan  Experiment: Screening  184. pEL3S15 - AmpR - T0  Result: Contamination. Construct discontinued, different backbone will be used.    Participant: Hampus  Experiment: Assembly  139. Plac - B0034 - SYFP2    Participant: Julia, Hampus  Experiment: Restreak  139. pSB3T5 - PT5lac - B0034 - SYFP2  Result: Success!    Participant: Olle, Karl  Experiment: PCR purification  pUC-ORI  pEL8to4C15  1. pSB1C3 - red    Experiment: Ligation  pEL8to4C15  pSB1C3 - pUC - red    Participant: Tobias, Christoffer  Experiment: Restreak  MG1655 - SpecR - J23101 - cat-sacB    Experiment: Screening on acrylamide gel on plasmids digested with NotI  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034  183. pSB1C3 - Ptac    **Thursday 2 August**    Participant: Fredrik  Experiment: Glycerol stock and plasmid prep.  190. pSB1C3 - MarR(mut)  191. pSB1C3 - LexA(G85D)  192. pSB1C3 - OmpF    Participant: Joel  Experiment: Plasmid prep.   12. pSB1A2 - B0034  127. pEL3A18(FRT) - red    Experiment: Screening and O/N  138. pEL3C18 - PlacIQ - tetA - lacIs  Result: Good!    Participant: Christoffer  Experiment: Screening  196. MG1655 - SpecR - J23101 - cat-sacB  Result: Good!    Participant: Hampus  Experiment: Assembly and transformation  193. pEL3S17 - plac - B0034 - SYFP2 - AmpR - TT0  194. pEL3S17 - PT5lac - B0034 - SYFP2 - AmpR - TT0  195. pSB3T5 - Pxis - B0034 - SYFP2    Experiment: Glycerol stock and plasmid prep.  186. pJN3K1 - AmpR - TT0    Participant: Karl  Experiment: Restreak  pSB1C3 - pUC - red  pEL4C15    Participant: Joel, Caroline, Emy  Experiment: Screening and O/N  152. pEL3C18 - J23106 - tetA - lacIs  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  181. pEL3A18 - J23106 - CTX-M - SYFP2  Result: Good clones of all contructs!    Experiment: Restreak  181. pEL3A18 - J23106 - CTX-M - SYFP2    Participant: Julia  Experiment: Screening  139. pSB3T5 - PT5lac - B0034 - SYFP2  Result: No clones seem to be right.    Experiment: Assembly and transformation  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034    **Friday 3 August**    Participant: Karl, Arvid  Experiment: O/N  187. pSB1C3 - pUC - red  188. pEL4C15 - red    Participant: Julia  Experiment: Assembly and transformation  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  81. pSB1C3 - J23116 - B0034    Participant: Katarina, Caroline, Emy  Experiment: Plasmid prep.  152. pEL3A18 - PlacIQ - tetA - SYFP2  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  181. pEL3A18 - J23106 - CTX-M - SYFP2    Experiment: Screening  181. pEL3A18 - J23106 - CTX-M - SYFP2  Result: All bad.    Experiment: Transformation  181. pEL3A18 - J23106 - CTX-M - SYFP2    Experiment: Preparation for sequencing  119. pEL3A18 - PlacIQ - tetA - SYFP2  138. pEL3C18 - PlacIQ - tetA - lacIs  152. pEL3C18 - J23106 - tetA - lacIs  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  181. pEL3A18 - J23106 - CTX-M - SYFP2    Participant: Olle  Experiment: Streak from frozen  123. pEL3A18 - red  124. pEL3C18 - red  125. pEL3K18 - red  126. pEL3S18 - red    Participant: Christoffer  Experiment: Glycerol stock  196. MG1655 - SpecR - J23101 - cat-sacB    Experiment: O/N  198. MG1655 - SpecR - J23101 - cat-sacB (without λ-tet-plasmid)    Experiment: Streak 198 on sucrose and tetracycline to make sure it works as it is supposed to    Participant: Ellen  Experiment: Assembly  181. pEL3A18 - J23106 - CTX-M - SYFP2    Participant: Hampus  Experiment: Restreak  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0  194. pEL3S17 - PT5lac - B0034 - SYFP2 - AmpR - TT0  195. pSB3T5 - Pxis - B0034 - SYFP2    **Saturday 4 August**    Participant: Hampus  Experiment: Screening  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0  194. pEL3S17 - PT5lac - B0034 - SYFP2 - AmpR - TT0    Participant: Caroline, Katarina  Experiment: Restreak  181. pEL3A18 - J23106 - CTX-M - SYFP2    Participant: Christoffer  Experiment: Glycerol stock  198. MG1655 - SpecR - J23101 - cat-sacB (without λ-Tet-plasmid)    Participant: Arvid, Karl  Experiment: Screening  187. pSB1C3 - pUC - red  Result: Several good clones.    Experiment: Assembly  33. pSB1A3 - PT5lac - B0034    Experiment: Plasmid prep.  188. pEL4C15 - red    Experiment: O/N  187. pSB1C3 - pUC - red    Participant: Olle  Experiment: PCR amplification  AmpR  AmpR(FRT)  CamR  CamR(FRT)  SpecR  SpecR(FRT)  TetR  TetR(FRT)  KanR(FRT)    Experiment: Screening  AmpR  AmpR(FRT)  CamR  CamR(FRT)  SpecR  SpecR(FRT)  Result: All except CamR(FRT) looked good.    **Sunday 5 August**    Experiment: Glycerol stock and plasmid prep.  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0    Participant: Arvid  Experiment: Plasmid prep. of 187. pSB1C3 - pUC - red, PCR amplification of the insert, PCR purification, vacuum drying, cloning into pEL4C15 and transformation.    Experiment: Restreak  33. pSB1A3 - PT5lac - B0034    Participant: Olle  Experiment: Screening  tetR  tetR(FRT)  KanR(FRT)  Result: All had good clones.    Experiment: PCR purification and digestion with DpnI, SacI and SalI  AmpR  AmpR(FRT)  CamR  CamR(FRT)  SpecR  SpecR(FRT)  TetR  TetR(FRT)  KanR(FRT)    Participant: Caroline, Katarina  Experiment: Screening and O/N  181. pEL3A18 - J23106 - CTX-M - SYFP2  Result: Two good clones    **Monday 6 August**    Participant: Hampus, Fredrik  Experiment: Glycerol stock and plasmid prep.  81. pSB1C3 - J23116 - B0034  159. pSB1C3 - PLlacO - B0034    Participant: Ellen, Katarina  Experiment: O/N  55. pSB3T5 - J23106 - CTX-M    Experiment: Plasmid prep.  181. pEL3A18 - J23106 - CTX-M - SYFP2  34. J61002 - J23106  61. J61002 - J23110    Experiment: Preparation for sequencing  181. pEL3A18 - J23106 - CTX-M - SYFP2    Participant: Sabri, Lisa  Experiment: Assembly/cloning and transformation  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  98. pEL3K17 - PT5lac    Participant: Arvid  Experiment: O/N  33. pSB1A3 - PT5lac - B0034    **Tuesday 7 August**    Participant: Olle  Experiment: PCR amplification for sequencing, PCR purification  199. pEL4C15 - pUC - red    Participant: Anders, Niklas, Josefin  Experiment: Cloning, screening, PCR purification  TetM  Result: Correct length on gel    Participant: Anton  Experiment: Preparation for sequencing  81. pSB1C3 - OmpF(rsr)  159. pSB1C3 - PLlacO - B0034  139. pSB3T5 - PT5lac - B0034 - SYFP2  190. pSB1C3 - MarR(mut)  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0    Participant: Hampus, Fredrik  Experiment: Screening  12. pSB1A2 - B0034  35. J61002 - J23116  81. pSB1C3 - J23116 - B0034  86. pSB1A2 - PLlacO  159. pSB1C3 - PLlacO - B0034  Result: All right.    Participant: Sabri, Lisa  Experiment: Assembly and transformation  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034    Experiment: Restreak  98. pEL3K17 - PT5lac    **Wednesday 8 August**    Participant: Lisa, Sabri  Experiment: Screening  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  Result: Expected bands at 426bp, faint bands at around 400bp were observed but disappeared before a picture was taken of the gel. New gel will be run tomorrow.    Experiment: Restreak  98. pEL3K17 - PT5lac    Participant: Olle  Experiment: Screening  33. pSB1A3 - PT5lac -B0034  28. pSB1C3 - PT5lac (used as reference)  Result: 33 is incorrect, only contains the RBS.    Experiment: O/N  199. pEL4C15 - pUC - red    Participant: Josefin, Niklas  Experiment: Cloning  pSB1C3 - tetM    **Thursday 9 August**    Participant: Anna  Experiment: O/N  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034    Experiment: Plasmid prep.  199. pEL4C15 - pUC - red    Participant: Joakim  Experiment: Assembly and transformation  pSB1C3 - J23106 - B0034    Participant: Sabri  Experiment: Screening  98. pEL3K17 - PT5lac  Result: Has to be redone.    Participant: Olle  Experiment: Digestion with SacI and SalI  199. pEL4C15 - pUC - red    Experiment: O/N  28. pSB1C3 - PT5lac    Experiment: Assembly  101. pSB3T5 - J23110 - B0034 - SYFP2    Participant: Sabri, Joel, Katarina  Experiment: Mutagenesis  213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2  214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2  215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2  216. pEL3A18 - PlacIQ - tetA - linker - SYFP2  217. pEL3A18 - J23106 - tetA - linker - SYFP2  218. pEL3A18 - J23106 - tetA - linker - SYFP2  219. pEL3C18 - J23106 - tetA - linker - lacIs  220. pEL3C18 - J23106 - AAC(6') - linker - lacIs  221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs  222. pEL3C18 - PlacIQ - tetA - linker - lacIs    **Friday 10 August**    Participant: Hampus, Joel  Experiment: Screening  197. pEL3S17 - red  Result: Wrong bands.    Experiment: PCR amplification  pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0    Participant: Hampus  Experiment: Assembly and transformation  164. pSB3T5 - Pxis - B0034 - SYFP2  223. pSB3T5 - J23116 - B0034 - ImmR - TλT1    Participant: Joakim  Experiment: Restreak  79. pSB1C3 - J23106 - B0034    Participant: Sabri, Lisa  Experiment: PCR purification, ligation and transformation  213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2  214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2  215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2  216. pEL3A18 - PlacIQ - tetA - linker - SYFP2  217. pEL3A18 - J23106 - tetA - linker - SYFP2  218. pEL3A18 - J23106 - tetA - linker - SYFP2  219. pEL3C18 - J23106 - tetA - linker - lacIs  220. pEL3C18 - J23106 - AAC(6') - linker - lacIs  221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs  222. pEL3C18 - PlacIQ - tetA - linker - lacIs    Participant: Olle  Experiment: Screening  199. pEL4C15 - pUC - red (cut with SacI and SalI)  Result: Good!    Experiment: Assembly and transformation  230. pEL4A15 - pUC - red  231. pEL4K15 - pUC - red  232. pEL4S15 - pUC - red  233. pEL4A15(FRT) - pUC - red  234. pEL4C15(FRT) - pUC - red  235. pEL4K15(FRT) - pUC - red  236. pEL4S15(FRT) - pUC - red  237. pEL4T15(FRT) - pUC - red  238. pEL4T15 - pUC - red    Participant: Katarina  Experiment: Cloning and transformation  225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2    Participant: Anna, Fredrik  Experiment: Assembly and transformation  202. pSB1K3 - B0034(mod) - LexA(mut)  203. pSB1K3 - B0034(mod) - OmpF(rsr)  204. pSB1K3 - B0034(mod) - marR(mut)  211. pSB3T5 - PLlacO - B0034 - LexA(mut)    Participant: Anders  Experiment: Assembly and transformation  227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2    Participant: Niklas, Josefin  Experiment: Screening  pSB1C3 - tetM  Result: Gel failed.    **Saturday 11 August**    Participant: Olle  Experiment: Plasmid prep.  154. pSB1C3 - PT5lac  240. pEL3S17 - P - R - lacIQ    Experiment: Restreak  230. pEL4A15 - pUC - red  231. pEL4K15 - pUC - red  232. pEL4S15 - pUC - red  233. pEL4A15(FRT) - pUC - red  234. pEL4C15(FRT) - pUC - red  235. pEL4K15(FRT) - pUC - red  236. pEL4S15(FRT) - pUC - red  237. pEL4T15(FRT) - pUC - red  238. pEL4T15 - pUC - red    Participant: Hampus  Experiment: Assembly  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0  228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR    Experiment: Restreak  165. pSB1K3 - ImmR - TλT1  223. pSB3T5 - J23116 - B0034 - ImmR - TλT1    Participant: Anna, Karl  Experiment: Assembly and transformation  202. pSB1K3 - B0034(mod) - LexA(G58D)  203. pSB1K3 - B0034(mod) - OmpF(rsr)  204. pSB1K3 - B0034(mod) - marR(mut)    Experiment: Restreak  211. pSB3T5 - PLlacO - B0034 - LexA(G58D)    Experiment: O/N  79. pSB1C3 - J23106 - B0034  239. pSB1A3 - J23101 - B0034    Participant: Joel, Katarina  Experiment: Restreak  213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2  214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2  215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2  216. pEL3A18 - PlacIQ - tetA - linker - SYFP2  217. pEL3A18 - J23106 - tetA - linker - SYFP2  218. pEL3A18 - J23106 - tetA - linker - SYFP2  219. pEL3C18 - J23106 - tetA - linker - lacIs  220. pEL3C18 - J23106 - AAC(6') - linker - lacIs  221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs  222. pEL3C18 - PlacIQ - tetA - linker - lacIs  225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2    Participant: Olle, Arvid  Experiment: Screening  pBad/AraC amplified from MG1655 chromosome  Result: Good.    **Sunday 12 August**    Participant: Anders  Experiment: Restreak  226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2  227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2    Participant: Joakim  Experiment: Plasmid prep.  79. pSB1A3 - J23101 - B0034  239. pSB1A3 - J23101 - B0034    Experiment: Mutagenesis (addition of B0034 to PT5lac)    Participant: Anna  Experiment: Restreak  202. pSB1K3 - B0034(mod) - LexA(G58D)  203. pSB1K3 - B0034(mod) - OmpF(rsr)  204. pSB1K3 - B0034(mod) - marR(mut)    Experiment: Screening  211. pSB3T5 - PLlacO - B0034 - LexA(G58D)  Result: No correct bands!    Participant: Anders B  Experiment: Screening  BioBricked tetM  Result: Looks good.    Participant: Fredrik  Experiment: Screening and O/N  213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2  216. pEL3A18 - PlacIQ - tetA - linker - SYFP2  217. pEL3A18 - J23106 - CTX-M - linker - SYFP2  225. pEL3C18 - PlacIQ - tetA - linker - SYFP2  Result: All had good clones.    Participant: Olle  Experiment: Screening and O/N  214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2  215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2  218. pEL3A18 - J23106 - tetA - linker - SYFP2  Result: All had good clones.    Experiment: Screening  230. pEL4A15 - pUC - red  232. pEL4S15 - pUC - red  233. pEL4A15(FRT) - pUC - red  234. pEL4C15(FRT) - pUC - red  235. pEL4K15(FRT) - pUC - red  236. pEL4S15(FRT) - pUC - red  237. pEL4T15(FRT) - pUC - red  Result: Gel failed, has to be redone.    Participant: Katarina  Experiment: Screening and O/N  219. pEL3C18 - J23106 - tetA - linker - lacIs  220. pEL3C18 - J23106 - AAC(6') - linker - lacIs  221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs  222. pEL3C18 - PlacIQ - tetA - linker - lacIs  Result: All had good clones.    Participant: Hampus  Experiment: Screening  164. pSB3T5 - Pxis - B0034 - SYFP2  223. pSB3T5 - J23116 - ImmR - TλT1  Result: 223 right. One clone of 164 might be right.    **Monday 13 August**    Participant: Karl, Arvid  Experiment: Screening  101. pSB3T5 - J23110 - B0034 - SYFP2  33. pSB1C3 - PT5lac - B0034  Result: 101 good, 33 has to be screened again.    Participant: Anna, Fredrik  Experiment: Screening  202. pSB1K3 - B0034(mod) - LexA(G58D)  203. pSB1K3 - B0034(mod) - OmpF(rsr)  204. pSB1K3 - B0034(mod) - marR(mut)  Result: All clones of 204 were good, 203 had one good clone, 202 failed.    Participant: Olle  Experiment: Screening  230. pEL4A15 - pUC - red  232. pEL4S15 - pUC - red  233. pEL4A15(FRT) - pUC - red  234. pEL4C15(FRT) - pUC - red  235. pEL4K15(FRT) - pUC - red  236. pEL4S15(FRT) - pUC - red  237. pEL4T15(FRT) - pUC - red  Result: All good!    Experiment: O/N  203. pSB1K3 - B0034(mod) - OmpF(rsr)  230. pEL4A15 - pUC - red  232. pEL4S15 - pUC - red  233. pEL4A15(FRT) - pUC - red  234. pEL4C15(FRT) - pUC - red  235. pEL4K15(FRT) - pUC - red  236. pEL4S15(FRT) - pUC - red  237. pEL4T15(FRT) - pUC - red  238. pEL4T5 - pUC - red    Participant: Anders, Johan  Experiment: Screening  226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2  227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2  Result: Contaminated.    Participant. Sabri, Lisa  Experiment: Plasmid prep.  164. pSB3T5 - Pxis - B0034 - SYFP2  220. pEL3C18 - J23106 - AAC(6') - linker - lacIs  221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs  222. pEL3C18 - PlacIQ - tetA - linker - lacIs  223. pSB3T5 - J23116 - B0034 - ImmR - TλT1  225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2    Participant: Joel  Experiment: Glycerol stock and plasmid prep.  241. pEL3S17-laciq - red  242. pEL3K16-laciq - red    Participant: Katarina, Caroline  Experiment: Plasmid prep.  213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2  214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2  215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2  216. pEL3A18 - PlacIQ - tetA - linker - SYFP2  217. pEL3A18 - J23106 - tetA - linker - SYFP2  218. pEL3A18 - J23106 - tetA - linker - SYFP2  219. pEL3C18 - J23106 - tetA - linker - lacIs    Participant: Hampus  Experiment: Screening  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0  228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR  Result: All bad.    Participant: Anton  Experiment: Preparation for sequncing  164. pSB3T5 - Pxis - B0034 - SYFP2  213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2  214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2  215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2  216. pEL3A18 - PlacIQ - tetA - linker - SYFP2  217. pEL3A18 - J23106 - tetA - linker - SYFP2  218. pEL3A18 - J23106 - tetA - linker - SYFP2  219. pEL3C18 - J23106 - tetA - linker - lacIs  220. pEL3C18 - J23106 - AAC(6') - linker - lacIs  221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs  222. pEL3C18 - PlacIQ - tetA - linker - lacIs  223. pSB3T5 - J23116 - B0034 - ImmR - TλT1  225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2    **Tuesday 14 August**    Participant: Caroline, Katarina  Experiment: Assembly  248. pEL4C15 - J23110 - CTX-M - SYFP2  249. pEL4C15 - J23110 - tetA - SYFP2  250. pEL3C18 - J23116 - CTX-M - lacIs  251. pEL3C18 - J23116 - tetA - lacIs    Participant: Hampus  Experiment: BioBrick PCR, PCR purification and screening  AmpR  Result: Gave bands of the right length.    Experiment: Assembly (using newly BioBricked AmpR)  228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR  229. pSB3K3 - Plac - B0034 - SYFP2 - AmpR    Participant: Johan  Experiment: Assembly and transformation  202. pSB1K3 - B0034 - LexA(G85D)    Participant: Arvid  Experiment: Screening  33. pSB1C3 - B0034 - PT5lac  Result: Very good!    Participant: Lisa  Experiment: O/N  241. pEL3S17iq - red    Participant: Anton  Experiment: Preparation for sequencing  33. pSB1C3 - PT5lac B0034  79. pSB1C3 - J23106 - B0034  80. pSB1C3 - J23110 - B0034  101. pSB3T5 - J23110 - B0034 - SYFP2  125. pEL3C18 - red  127. pEL3A18(FRT) - red  128. pEL3C18(FRT) - red  129. pEL3K18(FRT) - red  182. pEL3C18 - J23106 - CTX-M - lacIs  203. pSB1K3 - B0034(mod) - ompF(rsr)  204. pSB1K3 - B0034(mod) - marR(mut)  223. pSB3T5 - J23116 - B0034 - ImmR - TλT1  224. pSB1C3 - tetM    Participant: Caroline, Katarina  Experiment: Plasmid prep.  230. pEL4A15 - pUC - red  232. pEL4S15 - pUC - red  233. pEL4A15(FRT) - pUC - red  234. pEL4C15(FRT) - pUC - red  235. pEL4K15(FRT) - pUC - red  236. pEL4S15(FRT) - pUC - red  237. pEL4T15(FRT) - pUC - red    Participant: Joel  Experiment: Promoter exchange PCR (and screening)  Plac -> CP6  Result: PCR failed.    Participant: Sabri, Lisa  Experiment: Plasmid prep.  53. pSB3T5 - Plac - B0034 - SYFP2  182. pEL3C18 - J23106 - CTX-M - lacIs  204. pSB1K3 - B0034(mod) - marR(mut)  223. pSB3T5 - J23116 - B0034 - ImmR - TλT1  236. pEL4S15(FRT) - pUC - red  238. pEL4T15 - pUC - red  241. pEL3S17iq - red  242. pEL3K16iq - red    Participant: Anders  Experiment: Screening  226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2  227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2  Result: Extra bands, different PCR protocol will be used.    Participant: Joakim  Experiment: Cloning and transformation  pEL3S17 - Plac - b0034 - SYFP2    **Wednesday 15 August**    Participant: Olle  Experiment: Preparation for sequencing  230. pEL4A15 - pUC - red  232. pEL4S15 - pUC - red  233. pEL4A15(FRT) - pUC - red  234. pEL4C15(FRT) - pUC - red  235. pEL4K15(FRT) - pUC - red  236. pEL4S15(FRT) - pUC - red  237. pEL4T15(FRT) - pUC - red  238. pEL4T15 - pUC - red    Experiment: Digestion with E+P  pBad    Participant: Joakim  Experiment: Assembly  206. pSB3T5 - J23101 - B0034  208. pSB3T5 - J23101 - B0034 - marR(mut)    Experiment: Transformation  211. pSB3T5 - PLlacO - B0034 - LexA(G85D)    Participant: Anton  Experiment: Restreak  246. pEL3K18 - J23106 - B0034 - TetM  247. pEL3K18 - J23110 - B0034 - TetM  pEL3S17 - R - lacIQ    Participant: Anders  Experiment: Screening  226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2  227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2  Result: Gel failed.    Participant: Sabri  Experiment: Transformation  98. pEL3K17 - PT5lac  228. pEL3S18 - Plac - B0034 - SYFP2 - AmpR  229. pSB3K3 - Plac - B0034 - SYFP2 - AmpR  248. pEL4C15 - J23110 - CTX-M - SYFP2  249. pEL4C15 - J23110 - tetA - SYFP2  250. pEL3C18 - J23116 - CTX-M - lacIs  251. pEL3C18 - J23116 - tetA - lacIs    Participant: Caroline, Katarina  Experiment: Plasmid prep.  33. pSB1C3 - PT5lac - B0034  101. pSB3T5 - J23110 - B0034 - SYFP2    Participant: Arvid  Experiment: O/N from frozen stock  123. pEL3A18 - red  124. pEL3C15 - red  125. pEL3K18 - red  126. pEL3S18 - red  127. pEL3A18(FRT) - red  128. pEL3C18(FRT) - red  129. pEL3K18(FRT) - red  130. pEL3S18(FRT) - red    Participant: Fredrik  Experiment: Screening and O/N  202. pSB1K3 - B0034(mod) - LexA(G85D)  203. pSB1K3 - B0034(mod) - OmpF(rsr)  Result: Both had good clones.    Participant: Anna, Fredrik  Experiment: Plasmid prep.  202. pSB1K3 - B0034(mod) - LexA(G85D)  203. pSB1K3 - B0034(mod) - OmpF    **Thursday 16 August**    Participant: Caroline, Katarina  Experiment: Plasmid prep.  123. pEL3A18 - red  124. pEL3C15 - red  125. pEL3K18 - red  126. pEL3S18 - red  128. pEL3C18(FRT) - red  129. pEL3K18(FRT) - red  130. pEL3S18(FRT) - red    Participant: Fredrik, Anna, Joakim  Experiment: O/N  252. MG1655 (Acceptor Cipro.R)  253. R1drd19 - C.R  254. R46 - T.R  255. pSa - K.R  256. N3T - T.R    Participant: Joakim  Experiment: Transformation  206. pSB3T5 - J23101 - B0034 - OmpF(rsr)  208. pSB3T5 - J23101 - B0034 - marR(mut)    Experiment: Cloning  245. pEL3S17 - Plac - B0034 - SYFP2  262. pEL3K16 - PT5lac - B0034    Experiment: Restreak  245. pEL3S17 - Plac - B0034 - SYFP2    Participant: Joel  Experiment: Digest and screening  60. pSB1A3 - B0032 - SYFP2  Result: No good clones.    Experiment: Mutagenesis PCR, screening, PCR purification, DPNI  260. pEL3C18 - J23106 - CTX-M(5'UTR) - lacIs  Result: Good!    Participant: Arvid  Experiment: Assembly  102. pSB3K5 - J23110 - B0034 - SYFP2 B1002    Experiment: PCR for insertion of lacIq  pEL4x15    **Friday 17 August**    Participant: Anna, Fredrik, Joakim, Karl  Experiment: Test of conjugation  253. R1drd19  254. R46  255. pSA  256. N3T    Participant: Caroline, Katarina  Experiment: Screening  248. pEL4C15 - J23110 - CTX-M - SYFP2  249. pEL4C15 - J23110 - tetA - SYFP2  250. pEL3C18 - J23116 - CTX-M - lacIs  251. pEL3C18 - J23116 - tetA - lacIs  Result: One clone of 250 was right, the others were all wrong.    Experiment: PCR purification  260. pEL3C18 - J23106 - CTX-M - linker - lacIs    Experiment: Transformation  60. pSB1A3 - B0032 - SYFP2  260. pEL3C18 - J23106 - CTX-M - linker - lacIs    Participant: Joakim  Experiment: Restreak  206. pSB3T5 - J23101 - B0034 - OmpF(rsr)  208. pSB3T5 - J23101 - B0034 - marR(mut)    Experiment: Transformation  245. pEL3S17 - Plac - B0034 - SYFP2  262. pEL3K16 - PT5lac - B0034    Participant: Sabri, Lisa  Experiment: Making competent MG1655 cells    Experiment: Transformation  79. pSB1C3 - J23106 - B0034  225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 (in MG1655)    Participant: Arvid  Experiment: Transformation  102. pSB3C5 - J23110 - B0034 - SYFP2    Participant: Hampus  Experiment: Screening and restreak  228. pEL3X17 - Plac - B0034 - SYFP2 - AmpR  229. pSB3K3 - Plac - B0034 - SYFP2 - AmpR    Experiment: Assembly  79. pSB3T5 - J23106 - B0034  164. pSB3T5 - Pxis - B0034 - SYFP2  Result: The parts 164 was assembled from turned out to be wrong.    **Saturday 18 August**    Participant: Joakim  Experiment: Restreak  245. pEL3S17 - Plac - B0034 - SYFP2  262. pEL3K16 - PT5lac - B0034    Experiment: Screening  245. pEL3S17 - Plac - B0034 - SYFP2  262. pEL3K16 - PT5lac - B0034  Result: Screening has to be redone.    Participant: Hampus  Experiment: Transformation  225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 (in MG1655)    Experiment: Restreak  79. pSB3T5 - J23106 - B0034    Participant: Hampus, Karl  Experiment: Screening  87. pSB1C3 - pBad  228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR  Result: 87 had no good clones, 228 has to be screened again due to contamination.    Participant: Karl  Experiment: O/N  87. pSB1C3 - pBad  pGEM - eforRed  pGEM - aeBlue  pGEM - cjBlue    Participant: Anna  Experiment: Screening and O/N  206. pSB3T5 - J23101 - B0034 - OmpF(rsr)  208. pSB3T5 - J23101 - B0034 - marR(mut)  211. pSB3T5 - PLlacO - B0034 - LexA(G85D)    Participant: Katarina, Caroline, Emy  Experiment: Screening  250. pEL3C18 - J23116 - CTX-M - lacIs  257. pEL4C15 - J23110 AAC(6') - linker - SYFP2  258. pEL4C15 - PlacIQ - CTX-M - linker - SYFP2  259. pEL4C15 - J23106 - CTX-M - linker - SYFP2  Result: All had good clones.    Experiment: O/N  257. pEL4C15 - J23110 AAC(6') - linker - SYFP2  258. pEL4C15 - PlacIQ - CTX-M - linker - SYFP2  259. pEL4C15 - J23106 - CTX-M - linker - SYFP2    Experiment: Glycerol stock and plasmid prep.  250. pEL3C18 - J23116 - CTX-M - lacIs    Experiment: Restreak  60. pSB1A3 - B0032 - SYFP2  250. pEL3C18 - J23116 - CTX-M - lacIs  260. pEL3C18 - J23106 - CTX-M - linker - lacIs    Experiment: Assembly and transformation  248. pEL4C15 - J23110 - CTX-M - SYFP2  249. pEL4C15 - J23110 - tetA - SYFP2  250. pEL3C18 - J23116 - CTX-M - lacIs  251. pEL3C18 - J23116 - tetA - lacIs    **Sunday 19 August**    Participant: Karl  Experiment: Plasmid prep.  263. pGEM - eforRed  264. pGEM - aeBlue  265. pGEM - cjBlue    Experiment: Cloning from PCR product  87. pSB1C3 - pBad/AraC    Participant: Caroline, Katarina  Experiment: Screening  60. pSB1A3 - B0032 - SYFP2  250. pEL3C18 - J23116 - CTX-M - lacIs  260. pEL3C18 - J23106 - CTX-M - linker - lacIs    Experiment: Plasmid prep.  257. pEL4C15 - J23110 - AAC(6') - linker - SYFP2  258. pEL4C15 - PlacIQ - CTX-M - linker - SYFP2  259. pEL4C15 - J23106 - CTX-M - linker - SYFP2    Experiment: Transformation  248. pEL4C15 - J23110 - CTX-M - SYFP2  249. pEL4C15 - J23110 - tetA - SYFP2  250. pEL3C18 - J23116 - CTX-M - lacIs  251. pEL3C18 - J23116 - tetA - lacIs    Experiment: O/N  60. pSB1A3 - B0032 - SYFP2  228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR  250. pEL3C18 - J23116 - CTX-M - lacIs  260. pEL3C18 - J23106 - CTX-M - linker - lacIs    **Monday 20 August**    Participant: Caroline, Katarina  Experiment: Plasmid prep.  60. pSB1A3 - B0032 - SYFP2  228. åEL3S17 - Plac - B0034 - SYFP2 - AmpR  250. pEL3C18 - J23116 - CTX-M - lacIs  260. pEL3C18 - J23106 - CTX-M - linker - lacIs    Experiment: O/N  60. pSB1A3 - B0032 - SYFP2    **Tuesday 21 August**    Participant: Anna, Fredrik  Experiment: Assembly and transformation  204. pSB1K3 - B0034(mod) - marR(mut)  206. pSB3T5 - J23101 - B0034 - OmpF(rsr)  207. pSB3T5 - J23110 - B0034 - OmpF(rsr)  208. pSB3T5 - J23101 - B0034 - marR(mut)  211. pSB3T5 - PLlacO - B0034 - LexA(G85D)  261. pSB3K3 - PT5lac - B0034 - LexA(G85D)    Experiment: O/N  152. pEL3C18 - J23106 - tetA - lacIs  153. pJN3C1 - Red  154. pSB1C3 - PT5lac  155. pSB1A2 - lacI  156. pJN3C1 - AmpR    Participant: Karl  Experiment: Assembly and transformation  266. pSB1C3 - eforRed  267. pSB1C3 - aeBlue  268. pSB1C3 - cjBlue    Participant: Arvid  Experiment: Mutagenesis  pEL3K16 - redq (from red)  pEL8(A-T)17(FRT) - redq    Experiment: Mutagenesis  pEL3K16iq - red -> redq  pEL4[A-T]17(FRT) - red -> redq  Result: No band on gel.    Participant: Caroline, Katarina, Emy  Experiment: Plasmid prep.  228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR    Experiment: O/N  58. pSB3T5 - J23116 - CTX-M  114. pSB3T5 - J23110 - CTX-M  134. pSB1A3 - J23116 - tetA  189. pSB4K5 - lacIs    Experiment: Digestion with notI  12. pSB1A2 - B0034  79. pSB1C3 - J23106 - B0034    Experiment: Streak from frozen stock  3. pSB1C3 - red  39. pSB1K3 - B0034 - SYFP2  58. pSB3T5 - J23116 - CTX-M  114. pSB3T5 - J23110 - CTX-M  132. pSB1C3 - Pxis  134. pSB1A3 - J23116 - tetA  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  179. pEL3C18 - PlacIQ - AAC(6')- linker - lacIs  189. pSB4K5 - lacIs  219. pEL3C18 - J23106 - tetA - linker - lacIs  220. pEL3C18 - J23106 - AAC(6') - linker - lacIs  221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs  222. pEL3C18 - PlacIQ - tetA - linker - lacIs  225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2    Participant: Julia, Sabri  Experiment: Transformation  MG1655 225 + sRNA  MG1655 257 + sRNA  Result: Positive controll failed, has to be redone.    Participant: Sabri, Julia  Experiment: Making competent MG1655 cells containing reporter systems 225 (SYFP2) and 257 (lacIs)    Experiment: Transforation of randomized sRNAs into previously made competent cells.    **Thursday 23 August**    Participant: Joakim  Experiment: Screening  245. pEL3S17 - Plac - B0034 - SYFP2  262. pEL3K16 - PT5lac B0034  Result: No correct band.    Participant: Joel  Experiment: Assembly  301. pSB3T5 - Plac - B0032 - SYFP2  302. pSB3T5 - PlaciQ - B0032 - SYFP2  303. pSB3T5 - PT5lac - B0032 - SYFP2  304. pSB3T5 - PLlacO - B0032 - SYFP2  305. pSB3T5 - J23101 - B0032 - SYFP2  306. pSB3T5 - J23106 - B0032 - SYFP2  307. pSB3T5 - J23110 - B0032 - SYFP2  308. pSB3T5 - J23116 - B0032 - SYFP2  309. pSB3T5 - Ptac - B0032 - SYFP2  310. pSB3T5 - Pxis - B0032 - SYFP2    Participant: Joel, Karl  Experiment: Restreak  251. pEL3C18 - J23116 - tetA - lacIs  300. pEL3C18 - J23116 - CTX-M - linker - lacIs  301. pSB3T5 - Plac - B0032 - SYFP2  302. pSB3T5 - PlaciQ - B0032 - SYFP2  303. pSB3T5 - PT5lac - B0032 - SYFP2  304. pSB3T5 - PLlacO - B0032 - SYFP2  305. pSB3T5 - J23101 - B0032 - SYFP2  306. pSB3T5 - J23106 - B0032 - SYFP2  307. pSB3T5 - J23110 - B0032 - SYFP2  308. pSB3T5 - J23116 - B0032 - SYFP2  309. pSB3T5 - Ptac - B0032 - SYFP2  310. pSB3T5 - Pxis - B0032 - SYFP2  311. pSB3T5 - B0032 - SYFP2  316. pEL3A18 - J23110 - CTX-M - SYFP2  317. pEL3A18 - Plac - B0034 - SYFP2 - AmpR - T0    Participant: Caroline, Katarina  Experiment: Assembly and transformation  248. pEL4C15 - J23110 - CTX-M - SYFP2  249. pEL4C15 - J23110 - tetA - SYFP2  250. pEL3C18 - J23116 - CTX-M - lacIs  251. pEL3C18 - J23116 - tetA - lacIs  311. pSB3T5 - B0032 - SYFP2    Experiment: Plasmid prep.  3. pSB3T5 - red  39. pSB1K3 - B0034 - SYFP2  58. pSB3T5 - J23116 - CTX-M  114. pSB3T5 - J23110 - CTX-M  132. pSB1C3 - Pxis  134. pSB1A3 - J23116 - tetA  189. pSB4K5 - lacIs    Experiment: Mutagenesis  250. pEL3C18 - J23116 - CTX-M - lacIs    Participant: Arvid  Experiment: Mutagenesis  pEL3K16iq - red -> redq  pEL4[A-T]17(FRT) - red -> redq    Participant: Anna, Fredrik  Experiment: Screening and O/N  204. pSB1K3 - B0034(mod) - marR(mut)  206. pSB3T5 - J23101 - B0034 - OmpF(rsr)  207. pSB3T5 - J23110 - B0034 - OmpF(rsr)  208. pSB3T5 - J23101 - B0034 - marR(mut)  211. pSB3T5 - PLlacO - B0034 - LexA(G85D)  261. pSB3K3 - PT5lac - B0034 - LexA(G85D)  Result: 204, 208 and 211 had good clones, 206, 207 and 261 did not.    Participant: Julia  Experiment: Assembly and transformation  164. pSB3T5 - Pxis - B0034 - SYFP2    **Friday 24 August**    Participant: Pikkei, Karl  Experiment: Cloning  266. pSB1C3 - eforRed  267. pSB1C3 - aeBlue  268. pSB1C3 - cjBlue  320. pSB1C3 - PT5Imm    Participant: Hampus  Experiment: Transformation  MG1655 258  MG1655 259    Experiment: Restreak  164. pSB3T5 - Pxis - B0034 - SYFP2    Experiment: O/N  MG1655 225  MG1655 257    Participant: Joakim  Experiment: Screening  245. pEL3S17 - Plac - B0034 - SYFP2  262. pEL3K16iq - PT5lac - B0034  Result: All wrong.    Participant: Caroline, Katarina  Experiment: PCR purification  300. pEL3C18 - J23116 - CTX-M - linker - lacIs    Experiment: Streak from frozen stock  60. pSB1A3 - B0032 - SYFP2    Experiment: Screening  12. pSB1A2 - B0034  79. pSB3T5 - J23106 - B0034    Experiment: Transforming  300. pEL3C18 - J23116 - CTX-M - linker - lacIs  251. pEL3C18 - J23116 - tetA - lacIs  311. pSB3T5 - B0032 - SYFP2    Participant: Joel  Experiment: Assembly  316. pEL3A18 - J23110 - CTX-M - SYFP2  317. pEL3A18 - J23110 - tetA - SYFP2    Experiment: Transformation  301. pSB3T5 - Plac - B0032 - SYFP2  302. pSB3T5 - PlaciQ - B0032 - SYFP2  303. pSB3T5 - PT5lac - B0032 - SYFP2  304. pSB3T5 - PLlacO - B0032 - SYFP2  305. pSB3T5 - J23101 - B0032 - SYFP2  306. pSB3T5 - J23106 - B0032 - SYFP2  307. pSB3T5 - J23110 - B0032 - SYFP2  308. pSB3T5 - J23116 - B0032 - SYFP2  309. pSB3T5 - Ptac - B0032 - SYFP2  310. pSB3T5 - Pxis - B0032 - SYFP2  316. pEL3A18 - J23110 - CTX-M - SYFP2  317. pEL3A18 - J23110 - tetA - SYFP2    **Saturday 25 August**    Participant: Hampus, Sabri  Experiment: Screening  165. pSB3T5 - Pxis - B0034 - SYFP2    Experiment: Restreak  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0  318. pEL3S16 - Plac - B0034 SYFP2 - AmpR - TT0  320. pSB1C3 - PT5Imm    Experiment: Transformation of sRNA into MG1655 containing 257    Participant: Fredrik  Experiment: Assembly and transformation  202. pSB1K3 - B0034(mod) - LexA(G85D)  203. pSB1K3 - B0034(mod) - OmpF(rsr)  206. pSB3T5 - J23101 - B0034 - OmpF(rsr)  207. pSB3Y5 - J23110 - B0034 - OmpF(rsr)  261. pSB3K3 - PT5lac - B0034 - LexA(G85D)    Participant: Caroline, Katarina  Experiment: Transformation  225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2    Participant: Lisa  Experiment: Restreak  MG1655 258 pEL4C15 - PlacIQ - CTX-M - linker - SYFP2  MG1655 259 pEL4C15 - J23106 - CTX-M - linker - SYFP2    **Sunday 26 August**    Participant: Joel  Experiment: Screening  301. pSB3T5 - Plac - B0032 - SYFP2  302. pSB3T5 - PlaciQ - B0032 - SYFP2  303. pSB3T5 - PT5lac - B0032 - SYFP2  304. pSB3T5 - PLlacO - B0032 - SYFP2  305. pSB3T5 - J23101 - B0032 - SYFP2  306. pSB3T5 - J23106 - B0032 - SYFP2  307. pSB3T5 - J23110 - B0032 - SYFP2  308. pSB3T5 - J23116 - B0032 - SYFP2  309. pSB3T5 - Ptac - B0032 - SYFP2  310. pSB3T5 - Pxis - B0032 - SYFP2  311. pSB3T5 - B0032 - SYFP2  Result: Empty, new gel tomorrow.    Participant: Caroline, Katarina  Experiment: Transformation  225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 with 92. sRNA    Experiment: Restreak  202. pSB1K3 - B0034(mod) - LexA(G85D)  203. pSB1K3 - B0034(mod) - OmpF(rsr)  261. pSB3K3 - PT5lac - B0034 - LexA(G85D)    Experiment: Screening  251. pEL3C18 - J23116 - tetA - lacIs  300. pEL3C18 - J23116 - CTX-M - linker - lacIs  316. pEL3A18 - J23110 - CTX-M - SYFP2  317. pEL3A18 - J23110 - tetA - SYFP2  Result: All but 251 had good clones.    Experiment: O/N  300. pEL3C18 - J23116 - CTX-M - linker - lacIs  316. pEL3A18 - J23110 - CTX-M - SYFP2  317. pEL3A18 - J23110 - tetA - SYFP2    Experiment: Phusion PCR  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  179. pEL3C18 - PlacIQ - AAC(6')- linker - lacIs  219. pEL3C18 - J23106 - tetA - linker - lacIs  220. pEL3C18 - J23106 - AAC(6') - linker - lacIs  221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs  222. pEL3C18 - PlacIQ - tetA - linker - lacIs  225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2  257. pEL4C15 - J23110 - AAC(6') - linker - SYFP2  228. pEL4C15 - PlacIQ - CTX-M - linker - SYFP2  259. pEL4C15 - J23106 - CTX-M - linker - SYFP2  260. pEL3C18 - J23106 - CTX-M - linker - lacIs    Participant: Hampus  Experiment: Transformation  sRNA    Experiment: Restreak  206. pSB3T5 - J23101 - B0034 - OmpF(rsr)  207. pSB3Y5 - J23110 - B0034 - OmpF(rsr)    Experiment: Assembly  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - T0  318 - pEL3S16 - Plac - B0034 - SYFP2 - AmpR - T0    **Monday 27 August**    Participant: Joel, Fredrik  Experiment: Screening  301. pSB3T5 - Plac - B0032 - SYFP2  302. pSB3T5 - PlaciQ - B0032 - SYFP2  303. pSB3T5 - PT5lac - B0032 - SYFP2  304. pSB3T5 - PLlacO - B0032 - SYFP2  305. pSB3T5 - J23101 - B0032 - SYFP2  306. pSB3T5 - J23106 - B0032 - SYFP2  307. pSB3T5 - J23110 - B0032 - SYFP2  308. pSB3T5 - J23116 - B0032 - SYFP2  309. pSB3T5 - Ptac - B0032 - SYFP2  310. pSB3T5 - Pxis - B0032 - SYFP2  311. pSB3T5 - B0032 - SYFP2  Result: All had good clones.    Participant: Fredrik  Experiment: Restreak  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0  318. pEL3S16 - Plac - B0034 SYFP2 - AmpR - TT0    Participant: Joakim  Experiment: O/N  256. N3T    Participant: Julia, Hampus  Experiment: Plasmid prep.  164. pSB3T5 - Pxis - B0034 - SYFP2    **Tuesday 28 August**    Participant: Hampus, Julia  Experiment: Screening  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0  Result: Good.    Experiment: Restreak  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0    Experiment: O/N  164. pSB3T5 - Pxis - B0034 - SYFP2    Participant: Sabri  Experiment: Transformation  225+92 (reporter system + sRNA)  257+92 (reporter system + sRNA)  225+79 (to measure fitness cost for carrying two plasmids)  257+79 (to measure fitness cost)    Participant: Olle  Experiment: Screening  87. pSB1C3 - pBad  Result: All bands wrong.    Participant: Joel, Karl  Experiment: Mutagenesis, screening and PCR purification  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  179. pEL3C18 - PlacIQ - AAC(6')- linker - lacIs  219. pEL3C18 - J23106 - tetA - linker - lacIs  220. pEL3C18 - J23106 - AAC(6') - linker - lacIs  221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs  222. pEL3C18 - PlacIQ - tetA - linker - lacIs  260. pEL3C18 - J23106 - CTX-M - linker - lacIs  Screening: Gel looked OK, but the concentration after the PCR purification gave negative results.    Participant: Joakim  Experiment: Screening  202. pSB1K3 - B0034(mod) - LexA(G85D)  261. pSB3K3 - PT5lac - B0034 - LexA(G85D)  Result: No good bands.    Experiment: Viability test of 256. MG1655/N3T    **Wednesday 29 August**    Participant: Joel  Experiment: Screening  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  179. pEL3C18 - PlacIQ - AAC(6')- linker - lacIs  219. pEL3C18 - J23106 - tetA - linker - lacIs  220. pEL3C18 - J23106 - AAC(6') - linker - lacIs  221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs  222. pEL3C18 - PlacIQ - tetA - linker - lacIs  260. pEL3C18 - J23106 - CTX-M - linker - lacIs  320. pSB1C3 - pT5Imm  Result: Gel showed no bands.    Participant: Joakim  Experiment: Transformation  M13mp18    Experiment: O/N  MG1655 - pSa  MG1655 - R1drd19  MG1655 (cipro resistant)    Experiment: Counting colonies for the conjugation test    **Thursday 30 August**    Participant: Fredrik  Experiment: Screening and O/N  202. pSB1K3 - B0034(mod) - LexA(G85D)  206. pSB3T5 - J23101 - B0034 - OmpF(rsr)  261. pSB3K3 - PT5lac - B0034 - LexA(G85D)    Participant: Joel, Karl, Julia, Hampus  Experiment: Screening and O/N  305. pSB3T5 - J23101 - B0032 - SYFP2    Experiment: Mutagenesis  178. pSB3C18 - J23116 - AAC(6') - linker - lacIs  179. pSB3C18 - PlacIQ - AAC(6')- linker - lacIs  219. pEL3C18 - J23106 - tetA - linker - lacIs  220. pEL3C18 - J23106 - AAC(6') - linker - lacIs  221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs  222. pEL3C18 - PlacIQ - tetA - linker - lacIs  260. pEL3C18 - J23106 - CTX-M - linker - lacIs    Experiment: Digestion with notI and screening  302. pSB3T5 - placiQ - B0032 - SYFP2  303. pSB3T5 - pT5lac - B0032 - SYFP2  304. pSB3T5 - PLlacO - B0032 - SYFP2  305. pSB3T5 - J23101 - B0032 - SYFP2  306. pSB3T5 - J23106 - B0032 - SYFP2  307. pSB3T5 - J23110 - B0032 - SYFP2  308. pSB3T5 - J23116 - B0032 - SYFP2  309. pSB3T5 - ptac - B0032 - SYFP2  310. pSB3T5 - pxis - B0032 - SYFP2  Result: Bad gel.    Participant: Julia, Hampus  Experiment: PCR purification  RFP    Experiment: Plasmid prep.  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0    Participant: Joakim  Experiment: Conjugation test on 252+253 and 252+255    **Friday 31 August**    Participant: Joel  Experiment: Plasmid prep.  305. pSB3T5 - J23101 - B0032 - SYFP2    Experiment: Mutagenesis  317. pEL3A18 - J23110 - tetA - SYFP2    Experiment: PCR purification  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs  219. pEL3C18 - J23106 - tetA - linker - lacIs  220. pEL3C18 - J23106 - AAC(6') - linker - lacIs    Participant: Hampus  Experiment: Cloning to 1C3  J23101 - Spot42  J23106 - AAC(6') - linker - SYFP2  Plac - B0034 - SYFP2  PT5lac - B0034  J23116 - AAC(6') - linker - lacIs    Participant: Johan  Experiment: Mutagenesis  pSB2K3 - tet inv. (H64A)  pSB2K3 - tet inv. (E147A)  pSB2K3 - tet inv. (H100A)  pSB3T5 - J23110 - tet inv. B0034 - SYFP2 (H64A)  pSB3T5 - J23110 - tet inv. B0034 - SYFP2 (E147A)  pSB3T5 - J23110 - tet inv. B0034 - SYFP2(H100A)    Participant: Julia  Experiment: Assembly  325. pSB1C3 - J23101 - Spot42  326. pSB1C3 - J23106 - AAC(6') - linker - SYFP2  327. pSB1C3 - Plac - B0034 - SYFP2 - AmpR - TT0    Participant: Joakim, Fredrik  Experiment: Preparation of competent cells  DA25015  DA3006    **Saturday 1 September**    Participant: Julia, Hampus  Experiment: Lambda red PCR  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0    **Sunday 2 September**    Participant: Hampus  Experiment: Screening  262. pEL3K16 - PT5lac - B0034  325. pSB1C3 - Plac - B0034 - SYFP2  326. pEL3K16 - PT5lac - B0034  327. pSB1C3 - Plac - B0034 - SYFP2 - AmpR - TT0    **Monday 3 September**    Participant: Sabri  Experiment: Screening  262. pEL3K16 - PT5lac - B0034  325. pSB1C3 - J23101 - Spot42  326. pSB1C3 - J23106 - AAC(6') - linker - SYFP2  327. pSB1C3 - Plac - B0034 - SYFP2 - AmpR - TT0    Participant: Caroline, Katarina, Joel  Experiment: PCR purification, DpnI treatment, PCR purification  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0  221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs  222. pEL3C18 - PlacIQ - tetA - linker - lacIs  260. pEL3C18 - J23106 - CTX-M - linker - lacIs    Participant: Assembly  331. pEL3A18 - J23110 - tetA - linker - SYFP2    Participant: Hampus  Experiment: Isolation of non-fluorescent sRNA    **Tuesday 4 September**    Participant: Caroline, Katarina, Joel, Emy  Experiment: Assembly and transformation  336. pSB3S5 - Plac - B0032 - SYFP2  337. pSB3S5 - PlaciQ - B0032 - SYFP2  338. pSB3S5 - T5lac - B0032 - SYFP2  339. pSB3S5 - PLlacO - B0032 - SYFP2   340. pSB3S5 - J23101 - B0032 - SYFP2  341. pSB3S5 - J23106 - B0032 - SYFP2   342. pSB3S5 - J23110 - B0032 - SYFP2  343. pSB3S5 - J23116 - B0032 - SYFP2  344. pSB3S5 - Ptac - B0032 - SYFP2  345. pSB3S5 - Pxis - B0032 - SYFP2    Experiment: Transformation  331. pEL3A18 - J23110 - tetA - linker - SYFP2    Experiment: Lambda red into DA24100 and DA26250  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0    Participant: Hampus  Experiment: Lambda red PCR  180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2  213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2    **Wednesday 5 September**    Participant: Olle  Experiment: O/N  349. pJNEG04 - miniF - SYFP2 - CTX-M    Participant: Anton  Experiment: Screening  332. pEL4T15 - PLlacO - B0034 - TAL2  334. pSB3K3 - PT5lac - B0034 - TAL1    Experiment: Restreak  346. pSB1K3 - B0034 - TAL2    Participant: Hampus  Experiment: Transformation, plasmid prep.  sRNA  Spot42    Participant: Joel, Anna, Karl  Experiment: Transformation  280. pEL4A15Iq - pUC - red  281. pEL4C15Iq - pUC - red  283. pEL4S15Iq - pUC - red  284. pEL4T15Iq - pUC - red  285. pEL4A15(FRT)Iq - pUC - red  286. pEL4C15(FRT)Iq - pUC - red  287. pEL4K15(FRT)Iq - pUC - red  288. pEL4S15(FRT)Iq - pUC - red    Experiment: Restreak  331. pEL3A18 - J23110 - tetA - linker - SYFP2  336. pSB3S5 - Plac - B0032 - SYFP2  337. pSB3S5 - PlaciQ - B0032 - SYFP2  338. pSB3S5 - T5lac - B0032 - SYFP2  339. pSB3S5 - PLlacO - B0032 - SYFP2   340. pSB3S5 - J23101 - B0032 - SYFP2  341. pSB3S5 - J23106 - B0032 - SYFP2   342. pSB3S5 - J23110 - B0032 - SYFP2  343. pSB3S5 - J23116 - B0032 - SYFP2  344. pSB3S5 - Ptac - B0032 - SYFP2  345. pSB3S5 - Pxis - B0032 - SYFP2    **Thursday 6 September**    Participant: Joel  Experiment: Screening  331. pEL3A18 - J23110 - tetA - linker - SYFP2  336. pSB3S5 - Plac - B0032 - SYFP2  337. pSB3S5 - PlaciQ - B0032 - SYFP2  338. pSB3S5 - T5lac - B0032 - SYFP2  339. pSB3S5 - PLlacO - B0032 - SYFP2   340. pSB3S5 - J23101 - B0032 - SYFP2  341. pSB3S5 - J23106 - B0032 - SYFP2   342. pSB3S5 - J23110 - B0032 - SYFP2  343. pSB3S5 - J23116 - B0032 - SYFP2  344. pSB3S5 - Ptac - B0032 - SYFP2  345. pSB3S5 - Pxis - B0032 - SYFP2  Result: Screening failed.    Experiment: Restreak  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0    Participant: Sabri, Lisa, Hampus  Experiment: Transformation, plasmid prep  257. pEL4C15 - J23110 - AAC(6') - linker - SYFP2    Experiment: Restreak  non-fluorescent sRNA clones    Experiment: Mutagenesis  24. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2  226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2  227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2    **Friday 7 September**    Participant: Hampus  Experiment: Screening  326. pSB1C3 - J23106 - AAC(6') - linker - SYFP2  327. pSB1C3 - Plac - B0034 - SYFP2 - AmpR - TT0  348. pSB1C3 - J23116 - AAC(6') - linker - lacIs    Participant: Hampus, Sabri  Experiment: Transformation  Randomized sRNA    Experiment: Restreak  Isolated sRNAs    Experiment: O/N  pSB1C3 - J23101 - Spot42    Participant: Joakim  Experiment: Ligation  202. pSB1K3 - B0034(mod) - LexA(G85D)  206. pSB3T5 - J23101 - B0034 - OmpF(rsr)  207. pSB3Y5 - J23110 - B0034 - OmpF(rsr)  208. pSB3T5 - J23101 - B0034 - MarR(mut)  211. pSB3T5 - PLlacO - B0034 - LexA(G85D)  261. pSB3K3 - PT5lac - B0034 - LexA(G85D)    Participant: Olle  Experiment: Mutagenesis of red to redq on  280. pEL4A15Iq - pUC - red  281. pEL4C15Iq - pUC - red  283. pEL4S15Iq - pUC - red  285. pEL4A15(FRT)Iq - pUC - red  286. pEL4C15(FRT)Iq - pUC - red  287. pEL4K15(FRT)Iq - pUC - red    **Saturday 8 September**    Participant: Joel, Joakim  Experiment: Screening  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0  lac:amp  Result: 193 showed only primer dimers, lac:amp had good clones.    Participant: Lisa, Sabri  Experiment: Transformation  sRNAs into MG1655  sRNAs into 225    Experiment: Prepare cell cultures for cell sorting using FACS    Participant: Joakim  Experiment: Transformation  202. pSB1K3 - B0034(mod) - LexA(G85D)  206. pSB3T5 - J23101 - B0034 - OmpF(rsr)  207. pSB3Y5 - J23110 - B0034 - OmpF(rsr)  208. pSB3T5 - J23101 - B0034 - MarR(mut)  211. pSB3T5 - PLlacO - B0034 - LexA(G85D)  261. pSB3K3 - PT5lac - B0034 - LexA(G85D)    Experiment: Screening  178. pEL3C18 - J23116 - AAC(6') - linker - lacIs  179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs  219. pEL3C18 - J23106 - tetA - linker - lacIs  220. pEL3C18 - J23106 - AAC(6') - linker lacIs  221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs  222. pEL3C18 - PlacIQ - tetA - linker - lacIs  260. pEL3C18 - J23106 - CTX-M - linker - lacIs  Result: No bands visible.    Participant: Sabri  Experiment: Screening  24. pSB2K3 - tet inv.  226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2  227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2    **Sunday 9 September**    Participant: Olle  Experiment: Screening  pEL4-series  TALENs  Result: Backbones looked good, TALENs gave no bands.    **Monday 10 September**    Participant: Joel, Olle, Pikkei  Experiment: Assembly and transformation  336. pSB3S5 - plac - B0032 - SYFP2  337. pSB3S5 - placiQ - B0032 - SYFP2  338. pSB3S5 - T5lac - B0032 - SYFP2  339. pSB3S5 - PLlacO - B0032 - SYFP2   340. pSB3S5 - J23101 - B0032 - SYFP2  341. pSB3S5 - J23106 - B0032 - SYFP2   342. pSB3S5 - J23110 - B0032 - SYFP2  343. pSB3S5 - J23116 - B0032 - SYFP2  344. pSB3S5 - Ptac - B0032 - SYFP2  357. pSB4C5 - J23110 - B0032 - SYFP2  358. pSB4K5 - J23110 - B0032 - SYFP2  359. pEL4T15 - J23110 - B0032 - SYFP2  360. pEL4C15 - J23110 - B0032 - SYFP2    Experiment: Lambda red PCR  193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0    Experiment: O/N  lac:amp    Participant: Anna, Fredrik  Experiment: Screening and O/N  202. pSB1K3 - B0034(mod) - LexA(G85D)  206. pSB3T5 - J23101 - B0034 - OmpF(rsr)  207. pSB3Y5 - J23110 - B0034 - OmpF(rsr)  208. pSB3T5 - J23101 - B0034 - MarR(mut)  211. pSB3T5 - PLlacO - B0034 - LexA(G85D)  261. pSB3K3 - PT5lac - B0034 - LexA(G85D)    Participant: Olle  Experiment: Assembly and transformation  333. pEL3K16iq - J23110 - B0034 - eforRed  241. pEL4S17iq - red  243. pEL4S17iq - J23110 - B0034 - eforRed    **Tuesday 11 September**    **Wednesday 12 September**    Participant: Olle, Karl, Anna, Joel  Experiment: Screening  336. pSB3S5 - plac - B0032 - SYFP2  337. pSB3S5 - placiQ - B0032 - SYFP2  338. pSB3S5 - T5lac - B0032 - SYFP2  339. pSB3S5 - PLlacO - B0032 - SYFP2   340. pSB3S5 - J23101 - B0032 - SYFP2  341. pSB3S5 - J23106 - B0032 - SYFP2   342. pSB3S5 - J23110 - B0032 - SYFP2  343. pSB3S5 - J23116 - B0032 - SYFP2  344. pSB3S5 - Ptac - B0032 - SYFP2  357. pSB4C5 - J23110 - B0032 - SYFP2  358. pSB4K5 - J23110 - B0032 - SYFP2  359. pEL4T15 - J23110 - B0032 - SYFP2  360. pEL4C15 - J23110 - B0032 - SYFP2    Participant: Joakim  Experiment: O/N  MG1655 225  MG1655 257  sRNA    **Thursday 13 September**    Participant: Hampus  Experiment: Screening  325. pSB1C3 - J23101 - Spot42  361. pSB1C3 - RFP - linker - SYFP2  362. pSB1C3 - J23116 - AAC(6') - linker - lacIs  Result: 325 and 361 gave good bands, 362 gave no bands at all.    Participant: Julia  Experiment: Transformation  sRNA    Participant: Emy, Caroline, Katarina  Experiment: Tranformation  336. pSB3S5 - Plac - B0032 - SYFP2  337. pSB3S5 - PlaciQ - B0032 - SYFP2  338. pSB3S5 - T5lac - B0032 - SYFP2  339. pSB3S5 - PLlacO - B0032 - SYFP2   340. pSB3S5 - J23101 - B0032 - SYFP2  341. pSB3S5 - J23106 - B0032 - SYFP2   342. pSB3S5 - J23110 - B0032 - SYFP2  343. pSB3S5 - J23116 - B0032 - SYFP2  344. pSB3S5 - Ptac - B0032 - SYFP2  345. pSB3S5 - Pxis - B0032 - SYFP2  357. pSB4C5 - J23110 - B0032 - SYFP2  358. pSB4K5 - J23110 - B0032 - SYFP2  359. pEL4T15 - J23110 - B0032 - SYFP2  360. pEL4C15 - J23110 - B0032 - SYFP2    Experiment: Restreak  336. pSB3S5 - Plac - B0032 - SYFP2  337. pSB3S5 - PlaciQ - B0032 - SYFP2  338. pSB3S5 - T5lac - B0032 - SYFP2  339. pSB3S5 - PLlacO - B0032 - SYFP2   340. pSB3S5 - J23101 - B0032 - SYFP2  341. pSB3S5 - J23106 - B0032 - SYFP2   342. pSB3S5 - J23110 - B0032 - SYFP2  343. pSB3S5 - J23116 - B0032 - SYFP2  344. pSB3S5 - Ptac - B0032 - SYFP2  345. pSB3S5 - Pxis - B0032 - SYFP2  360. pEL4C15 - J23110 - B0032 - SYFP2    **Friday 14 September**    Participant: Katarina, Caroline, Joel, Emy  Experiment: Mutagenesis  215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2  216. pEL3A18 - PlacIQ – tetA - linker - SYFP2  218. pEL3A18 - J23106 - tetA - linker - SYFP2  347. pEL3C18 - J23116 - tetA - linker - lacIs    Experiment: Screening and O/N  336. pSB3S5 - Plac - B0032 - SYFP2  337. pSB3S5 - PlaciQ - B0032 - SYFP2  338. pSB3S5 - T5lac - B0032 - SYFP2  339. pSB3S5 - PLlacO - B0032 - SYFP2   340. pSB3S5 - J23101 - B0032 - SYFP2  341. pSB3S5 - J23106 - B0032 - SYFP2   342. pSB3S5 - J23110 - B0032 - SYFP2  343. pSB3S5 - J23116 - B0032 - SYFP2  344. pSB3S5 - Ptac - B0032 - SYFP2  345. pSB3S5 - Pxis - B0032 - SYFP2  360. pEL4C15 - J23110 - B0032 - SYFP2    Participant: Julia  Experiment: O/N  MG1655 with 225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2  MG1655 with 257. pEL4C15 - J23110 - AAC(6') - linker - SYFP2    Experiment: Plasmid prep.  86. pSB1A2 - PLlacO  361. pSB1C3 - RFP - linker - SYFP2  362. pSB1C3 - J23116 - AAC(6') - linker - lacIs    **Saturday 15 September**    Participant: Julia  Experiment: Restreak, plasmid prep.  sRNAs    Experiment: Screening, PCR purification and digestion with DpnI  AC6 - Spot42    **Friday 21 September**    Participant: Joel  Experiment: Restreak  215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2  216. pEL3A18 PlacIQ – tetA - linker - SYFP2  218. pEL3A18 J23106 - tetA - linker - SYFP2  382. pSB4K5 - J23101 - B0032 - SYFP2  383. pEL4T15 - J23101 - B0032 - SYFP2  384. pEL8C15 - J23101 - B0032 - SYFP2    Experiment: Streak from frozen stock  336. pSB3S5 - Plac - B0032 - SYFP2  337. pSB3S5 - PlaciQ - B0032 - SYFP2  338. pSB3S5 - T5lac - B0032 - SYFP2  339. pSB3S5 - PLlacO - B0032 - SYFP2   340. pSB3S5 - J23101 - B0032 - SYFP2  341. pSB3S5 - J23106 - B0032 - SYFP2   342. pSB3S5 - J23110 - B0032 - SYFP2  343. pSB3S5 - J23116 - B0032 - SYFP2  344. pSB3S5 - Ptac - B0032 - SYFP2  345. pSB3S5 - Pxis - B0032 - SYFP2 |

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