iGEM 2020 — Team ECUST_China

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:ECUST China/Notebook

home

[![HOME](https://static.igem.org/mediawiki/2020/a/a7/T--ECUST_China--logo_chang.png)](https://2020.igem.org/Team%3AECUST_China)

#### [Team](https://2020.igem.org/Team%3AECUST_China/Members)

###### [Members](https://2020.igem.org/Team%3AECUST_China/Members)

###### [Collaborations](https://2020.igem.org/Team%3AECUST_China/Collaborations)

#### [Project](https://2020.igem.org/Team%3AECUST_China/Description)

###### [Description](https://2020.igem.org/Team%3AECUST_China/Description)

###### [Design](https://2020.igem.org/Team%3AECUST_China/Design)

###### [Experiments](https://2020.igem.org/Team%3AECUST_China/Experiments)

###### [Contribution](https://2020.igem.org/Team%3AECUST_China/Contribution)

###### [Attribution](https://2020.igem.org/Team%3AECUST_China/Attributions)

###### [Safety](https://2020.igem.org/Team%3AECUST_China/Safety)

#### [Results](https://2020.igem.org/Team%3AECUST_China/Results)

###### [Engineering](https://2020.igem.org/Team%3AECUST_China/Engineering)

###### [Demonstrate](https://2020.igem.org/Team%3AECUST_China/Demonstrate)

###### [Improve](https://2020.igem.org/Team%3AECUST_China/Improve)

#### [Parts](https://2020.igem.org/Team%3AECUST_China/Parts)

#### [Human](https://2020.igem.org/Team%3AECUST_China/Human%20Practices)

###### [Human Practices](https://2020.igem.org/Team%3AECUST_China/Human%20Practices)

###### [Education](https://2020.igem.org/Team%3AECUST_China/Education)

#### [Awards](https://2020.igem.org/Team%3AECUST_China/Awards)

###### [Hardware](https://2020.igem.org/Team%3AECUST_China/Hardware)

###### [Model](https://2020.igem.org/Team%3AECUST_China/Model)

###### [Implementation](https://2020.igem.org/Team%3AECUST_China/Implementation)

###### [Proof of concept](https://2020.igem.org/Team%3AECUST_China/Proof_Of_Concept)

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:ECUST China/Engineering

home

[![HOME](https://static.igem.org/mediawiki/2020/a/a7/T--ECUST_China--logo_chang.png)](https://2020.igem.org/Team%3AECUST_China)

#### [Team](https://2020.igem.org/Team%3AECUST_China/Members)

###### [Members](https://2020.igem.org/Team%3AECUST_China/Members)

###### [Collaborations](https://2020.igem.org/Team%3AECUST_China/Collaborations)

#### [Project](https://2020.igem.org/Team%3AECUST_China/Description)

###### [Description](https://2020.igem.org/Team%3AECUST_China/Description)

###### [Design](https://2020.igem.org/Team%3AECUST_China/Design)

###### [Experiments](https://2020.igem.org/Team%3AECUST_China/Experiments)

###### [Contribution](https://2020.igem.org/Team%3AECUST_China/Contribution)

###### [Attribution](https://2020.igem.org/Team%3AECUST_China/Attributions)

###### [Safety](https://2020.igem.org/Team%3AECUST_China/Safety)

#### [Results](https://2020.igem.org/Team%3AECUST_China/Results)

###### [Engineering](https://2020.igem.org/Team%3AECUST_China/Engineering)

###### [Demonstrate](https://2020.igem.org/Team%3AECUST_China/Demonstrate)

###### [Improve](https://2020.igem.org/Team%3AECUST_China/Improve)

#### [Parts](https://2020.igem.org/Team%3AECUST_China/Parts)

#### [Human](https://2020.igem.org/Team%3AECUST_China/Human%20Practices)

###### [Human Practices](https://2020.igem.org/Team%3AECUST_China/Human%20Practices)

###### [Education](https://2020.igem.org/Team%3AECUST_China/Education)

#### [Awards](https://2020.igem.org/Team%3AECUST_China/Awards)

###### [Hardware](https://2020.igem.org/Team%3AECUST_China/Hardware)

###### [Model](https://2020.igem.org/Team%3AECUST_China/Model)

###### [Implementation](https://2020.igem.org/Team%3AECUST_China/Implementation)

###### [Proof of concept](https://2020.igem.org/Team%3AECUST_China/Proof_Of_Concept)

![](https://static.igem.org/mediawiki/2020/b/bd/T--ECUST_China--engineering.png)

![1](https://static.igem.org/mediawiki/2020/d/d2/T--ECUST_China--quan.jpg)
![2](https://static.igem.org/mediawiki/2020/4/4e/T--ECUST_China--lab2.jpg)
![3](https://static.igem.org/mediawiki/2020/e/e7/T--ECUST_China--lab3.jpg)

![](https://static.igem.org/mediawiki/2020/1/1f/T--ECUST_China--doctor.gif)

![](https://static.igem.org/mediawiki/2020/1/18/T--ECUST_China--Engineering.png)

As we described in the experiment, before using D-E-tector to detect viruses or mutations, we need to carry out qualitative verification of DNA Walker and E-CRISPR respectively. Although it is only qualitative verification, its workload is very large. Especially under the influence of coronavirus, the time we can use for experiments is very limited. Fortunately, the qualitative verification of these two parts has been successful. In addition, the competition of the C/G/F triple chain, which is a series of two parts, has also been preliminarily verified.

Qualitative verification results of DNA Walker part

We started the experiment of DNA Walker at the end of September and independently designed the assembly procedure of magnetic beads and the reaction procedure of chain replacement. Our experimental results on October 9th show that DNA Walker can transform the input line T signal into the output C:F double-chain signal under a certain amount of line T and line W concentration and through appropriate reaction procedures. However, our experimental results are not perfect. In the experimental results on October 9th, we found that the positive control had obvious C:F double-stranded detection and the negative control also had weak C:F double-stranded signal, which made us notice the machine leakage of DNA Walker. Therefore, in the subsequent experiments, we hope to reduce the impact of leakage by reasonably designing the reaction program.

Qualitative verification results of E-CRISPR part

We verified the lineG cutting in PCR tube and on electrode respectively. Our experimental results show that, when the reaction occurs in an PCR tube, the C:G double strand can be cut by cas9 nickase, and some short segments are produced and can be shown in PAGE. We tried other reaction conditions, such as cutting only with lineF or lineG, and they could not produce such a cutting band. This shows that the cutting of C:G is specific, which is consistent with the design.

We also carried out verification experiments on the electrode. We found that when there was line C in the system, cutting would occur on the electrode and the chemical current would decrease, which was completely different from negative control and blank control. This shows that the cutting of C:G is also specialized when it is on the electrode, and it can indeed produce considerable chemical current changes. Of course, we have also noticed some problems such as surfactant and unstable electrode modification, and we will improve these parts in subsequent experiments.

The verification results of the C/F/G competition experiment

DNA Walker outputs C:F double strand to E-CRISPR, while E-CRISPR only needs lineC, which makes lineG need to compete with F chain. We also verified this part on the PCR tube and electrode respectively, and the results show that the G chain can compete with the C chain both in the PCR tube and electrode. Of course, at present, this kind of competition needs to be promoted by providing a certain amount of heat. We plan to make this part more efficient by prolonging the reaction time or changing the experimental design in the later stage.

**To sum up, we believe that we have achieved some certain results in engineering.**

![]()

#### ECUST\_China

EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Shanghai, China

[![](https://static.igem.org/mediawiki/2020/9/97/T--ECUST_China--ECUST_logo.png)](https://www.ecust.edu.cn/)
[![](https://static.igem.org/mediawiki/2020/7/79/T--ECUST_China--%E7%94%9F%E5%B7%A5-logo.jpg)](http://biotech.ecust.edu.cn/)

#### GET IN TOUCH

+86 17553856936

iGEM\_ECUST@outlook.com

[![](https://static.igem.org/mediawiki/2020/9/99/T--ECUST_China--031.png)](https://www.facebook.com/ecust.ecust)
[![](https://static.igem.org/mediawiki/2020/5/57/T--ECUST_China--01.png)](https://space.bilibili.com/633958833?share_medium=android&share_source=copy_link&bbid=XY7D24B7735A414A5DC4AB35DD72D2DE08FD4&ts=1603379493518)
[![](https://static.igem.org/mediawiki/2020/b/b2/T--ECUST_China--021.png)](https://mp.weixin.qq.com/s/b43sFIRw-VrWO82urP8zPA)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:ECUST China/Experiments

home

[![HOME](https://static.igem.org/mediawiki/2020/a/a7/T--ECUST_China--logo_chang.png)](https://2020.igem.org/Team%3AECUST_China)

#### [Team](https://2020.igem.org/Team%3AECUST_China/Members)

###### [Members](https://2020.igem.org/Team%3AECUST_China/Members)

###### [Collaborations](https://2020.igem.org/Team%3AECUST_China/Collaborations)

#### [Project](https://2020.igem.org/Team%3AECUST_China/Description)

###### [Description](https://2020.igem.org/Team%3AECUST_China/Description)

###### [Design](https://2020.igem.org/Team%3AECUST_China/Design)

###### [Experiments](https://2020.igem.org/Team%3AECUST_China/Experiments)

###### [Contribution](https://2020.igem.org/Team%3AECUST_China/Contribution)

###### [Attribution](https://2020.igem.org/Team%3AECUST_China/Attributions)

###### [Safety](https://2020.igem.org/Team%3AECUST_China/Safety)

#### [Results](https://2020.igem.org/Team%3AECUST_China/Results)

###### [Engineering](https://2020.igem.org/Team%3AECUST_China/Engineering)

###### [Demonstrate](https://2020.igem.org/Team%3AECUST_China/Demonstrate)

###### [Improve](https://2020.igem.org/Team%3AECUST_China/Improve)

#### [Parts](https://2020.igem.org/Team%3AECUST_China/Parts)

#### [Human](https://2020.igem.org/Team%3AECUST_China/Human%20Practices)

###### [Human Practices](https://2020.igem.org/Team%3AECUST_China/Human%20Practices)

###### [Education](https://2020.igem.org/Team%3AECUST_China/Education)

#### [Awards](https://2020.igem.org/Team%3AECUST_China/Awards)

###### [Hardware](https://2020.igem.org/Team%3AECUST_China/Hardware)

###### [Model](https://2020.igem.org/Team%3AECUST_China/Model)

###### [Implementation](https://2020.igem.org/Team%3AECUST_China/Implementation)

###### [Proof of concept](https://2020.igem.org/Team%3AECUST_China/Proof_Of_Concept)

![](https://static.igem.org/mediawiki/2020/9/95/T--ECUST_China--experiment.png)

![1](https://static.igem.org/mediawiki/2020/d/d2/T--ECUST_China--quan.jpg)
![2](https://static.igem.org/mediawiki/2020/4/4e/T--ECUST_China--lab2.jpg)
![3](https://static.igem.org/mediawiki/2020/e/e7/T--ECUST_China--lab3.jpg)

![](https://static.igem.org/mediawiki/2020/f/fb/T--ECUST_China--virus_war.gif)

D-E-tector experiment

Overview:

In order to detect the mutated new coronavirus, we can implement the following two schemes, or even a comprehensive scheme of the two schemes.

1. Design different T strands of DNA walkers to recognize the mutated new coronavirus, produce the same CF double strand, which is recognized by E-CRISPR and output the same MB signal, and react in different lanes of the digital microfluidic device.

2. Design different T strands of DNA walkers to recognize the mutated new coronavirus, and produce different CF double strands corresponding to (T), which are recognized and cut by different Cas9 nickases to generate the same MB signal.

3. Design different T strands of DNA walker to recognize the mutated new coronavirus, and produce different CF double strands corresponding to (T), which are recognized and cut by different Cas9 nickases to generate different electrical signals.

As the serial number increases, the greater the experimentally detectable flux, stability will be a huge challenge.

DNA walker experiment

After comprehensive consideration, in the experiment of the DNA walker part, we hope to verify the following hypotheses:

1. Magnetic beads saturation assembly LABC

2. T can be recognized by DNA walker to release CF double strand

3. Determine the detection limit of DNA walker

4. Measure the time required for the DNA walker reaction

Therefore, we designed the following experiment.

1. Sequence design

1.1 Target sequence design

![](https://static.igem.org/mediawiki/2020/3/36/T--ECUST_China--experiment-pic-T_sequence.png)

First, we use the primers of N-SARS-CoV-2 published by WHO and CDC as the prototype to design the sequence T (trigger seq).

1.2 DNA walker design

![](https://static.igem.org/mediawiki/2020/b/bc/T--ECUST_China--experiment-pic-Reaction-DNA_walker.png)

Secondly, based on the principle of DNA walker and the guiding significance of the modeling results, we designed a preliminary sequence. After consulting Ma Peiqiang, considering the experimental period and stability of DNA walker and other influencing factors, we decided to use the ABCF sequence as shown for qualitative verification and design the LWT sequence. According to the sequence T, the sequence L and the sequence W which are complementary to its part are designed. The final result is shown in the Table S1.

![](https://static.igem.org/mediawiki/2020/e/e4/T--ECUST_China--experiment-pic-DNA_walker_sequence.png)

2. Magnetic beads saturation assembly LABC

Add magnetic beads, AL, and BC into the eppendorf tube, and save the corresponding supernatant for PAGE gel electrophoresis. If AL bands appear in the supernatant AL, it proves that the magnetic beads are saturated to modify AL. If the BC band appears in the supernatant BC, it proves that the magnetic beads are saturated to modify the ABC sandwich structure. If there is no band in the supernatant after assembly and washing, it is proved that the magnetic beads LABC is washed clean.

3. T can be recognized by DNA walker to release CF double strand

When the magnetic beads LABC reacted, T was added to the positive control, and the same amount of ddH2O was added to the negative control. Take the supernatant for PAGE gel electrophoresis. If the positive control has a CF band and is brighter than the negative control CF band, the hypothesis is true.

4. Determine the detection limit of DNA walker

Dilute T gradually, and then add it to the reaction. The rest is the same as the experimental scheme of hypothesis 3. If a CF band is observed, it proves that the detection limit designed by the DNA walker is T.

5. Measure the time required for the DNA walker reaction

When the magnetic beads are added to the T reaction, different time gradients are set for measurement.

E-CRISPR experiment

Overview：
In this module, we hope we can confirm that line G can pair with line C instead of line F, and then, sgRNA can recognize it and lead Cas9 nickase to cut line G successfully, producing a relatively obvious change in electric signals. Therefore, we designed a four-part experiment.
At first, we tried to modify line G to the electrode efficiently. We set up experimental groups with different modification times and chose a condition that works best for subsequent experiments. Secondly, we directly added line C to the electrode which has been modified with line G, and then we added sgRNA and Cas9 nickase to cut line G. Next, it should be confirmed that line G, instead of line F, can pair with line C, and the cutting will still happen in this system. Finally, we try to make it work on the electrode plate.

![](https://static.igem.org/mediawiki/2020/4/43/T--ECUST_China--experiment-pic-ecrispr.png)

Sequence design

In our E-CRISPR system, the main components are line G, line F, line C, sgRNA, Cas9 nickase. Line C is paired with line F when they come into the E-CRISPR module because they are products of the DNA Walker module. And the sequences of them are also the same as which when they are in the DNA Walker module.

Then, line G, which is modified on the electrode plate, will compete with line F to pair with line C because line G has more complementary bases with line C than line F.

After that, we will add sgRNA and Cas9 nickase to this system. The sgRNA recognizing a specific sequence of line G will lead Cas9 nickase to be close to line C and line G. The Cas9 nickase can recognize the PAM site of line C, and cut line G specifically, which conduct the decrease of the signal. It should be noted that there are two PAM sites here, GGG and AGG, so we also designed the experiment to confirm which one is appropriate(refer to “validation on electrode; cut validation of line G”).

The specific sequences of line F, line G, line C, and sgRNA are showed on the blowing picture.

![](https://static.igem.org/mediawiki/2020/f/f5/T--ECUST_China--experiment-pic-Reaction-ecrisper-AGG.png)
![](https://static.igem.org/mediawiki/2020/1/13/T--ECUST_China--experiment-pic-Reaction-ecrisper-GGG.png)

Electrochemical workstation

In the E-CRISPR module, when we conduct the experiments on the electrode plate, we need the help of an electrochemical workstation. It is a small but sensitive equipment that can connect the electrode plate to the computer and read out the change of electric signals. Based on it, we can know that if the competition and cut of line G have happened.

![](https://static.igem.org/mediawiki/2020/6/66/T--ECUST_China--experiment-workstation_1.png)
![](https://static.igem.org/mediawiki/2020/6/66/T--ECUST_China--experiment-workstation_2.png)
![](https://static.igem.org/mediawiki/2020/6/66/T--ECUST_China--experiment-workstation_3.png)

Validation in PCR tube

Competition validation of line G

In this part, we designed six control groups, which perspectively contains line C, line G, line F, line C and line G, line F and line G, and line C and line F, while one experiment group contains line F/G/C.

All of these seven groups were incubated under room-temperature for x hours, and then we verified the result of competition through polyacrylamide gel electrophoresis. The imaging result showed that line G, instead of line F could pair with line C to some extent.

Besides, considering that the competition time is too long, we try to increase the efficiency of competition by heating and conjecture based on this result that the reason for the low-efficiency of competition is the low-efficiency of melting.

As we expect, melting and annealing can make the competition effect reach the same level as long-term incubation in a shorter time. So, solving the melting problem is our plan.

![](https://static.igem.org/mediawiki/2020/c/cb/T--ECUST_China--experiment-Competition_validation_of_line_G.png)

Cut validation of line G

In this part, we designed one experiment group that we directly added sgRNA, Cas9 nickase, and buffer to the system which only contains line G and line C, and three control groups. We perspectively only added sgRNA, Cas9 nickase to two of them, and added nothing to another one. The result, which was verified through polyacrylamide gel electrophoresis, showed that it was successful. In brief, the cut can be realized.

![](https://static.igem.org/mediawiki/2020/2/20/T--ECUST_China--experiment-Cut_validation_of_line_G-1.png)
![](https://static.igem.org/mediawiki/2020/e/e4/T--ECUST_China--experiment-Cut_validation_of_line_G-2.png)

Systematic validation

In this part, we added line F and line C into the system at first, after an hour, we added line G and still incubated the same time. Then, sgRNA and Cas9 nickase was added and reacted for 2 hours. Through polyacrylamide gel electrophoresis, we found that the cut could not be successful anymore. Considering that in the “competition validation” part, line G can compete with line F and pair with line C to some extent, a small amount of line G could be cut by Cas9 nickase. So, we conjecture that the line F solution or buffer were not pure enough. We may use a more pure line F solution and buffer to continue the subsequent experiments.

Validation on the electrode

Optimization of line G-modification time

We first carried out the detection experiment of the optimal modification time of line G on the electrode plate. We designed 8 groups of electrode plate experiments with different modification time of 2hr, 4hr, 6hr, 8hr, 10hr, 12hr, 24hr, and 36hr. The electric signal after the modification was detected. According to the baseline and peak value of the electric signal and considering the significance of the experimental results and the experimental period, we chose 2hr as the modification time for our follow-up experiments.

It can be seen from the figure that with the increase of the modification time, the detected electric signal gradually increased but the tendency is not worthy of prolonging the reaction time. Significant results could be obtained in the experiment, so considering the arrangement of the experiment cycle, we chose 2 hr for follow-up experiments.

![](https://static.igem.org/mediawiki/2020/3/3b/T--ECUST_China--experiment-Optimization_of_line_G-modification_time.png)

The decline of the electric signal

We first used line G to modify for 8 hours, then blocked it with MCH for 45 minutes, eluted, and measured the signal for the first time. Then we continued to measure the electric signal kept in the buffer at regular intervals until the electric signal dropped below 50% of the first measured value.

By measuring the attenuation of the electric signal after modification, it further explained that when the cutting verification was performed. The measured electric signal decreased by more than 10%, which was caused by the successful cutting.

![](https://static.igem.org/mediawiki/2020/5/52/T--ECUST_China--experiment-The_decline_of_the_electric_signal.png)

From the figure, it can be seen that at about 54hr, the electric signal dropped below 50% of the first measured result.

Cut validation of line G

We first used line G to modify for 2 hours, then blocked it with MCH for 45 minutes, eluted, and added complementary strands for 1 hr. And we performed the first signal measurement after elution. Then, we added sgRNA and Cas9 to cut the chain and performed the second signal measurement after elution.
For some reason, this validation is a partial success, and we tried to draw a conclusion of this outcome.

![](https://static.igem.org/mediawiki/2020/5/5f/T--ECUST_China--experiment-Cut_validation_of_line_G.png)

Systematic validation

The reaction system in this part was the same as the system of systematic validation in the EP tube, but line G was modified on the electrode plate. Through the change of signals, we can estimate whether the cut is successful or not. But we haven’t completed the validation of this part, we will continue to work on this in the future.

[For information about the experimental protocol, please click here.](https://static.igem.org/mediawiki/2020/3/3d/T--ECUST_China--experiment_protocol.pdf)

#### ECUST\_China

EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Shanghai, China

[![](https://static.igem.org/mediawiki/2020/9/97/T--ECUST_China--ECUST_logo.png)](https://www.ecust.edu.cn/)
[![](https://static.igem.org/mediawiki/2020/7/79/T--ECUST_China--%E7%94%9F%E5%B7%A5-logo.jpg)](http://biotech.ecust.edu.cn/)

#### GET IN TOUCH

+86 17553856936

iGEM\_ECUST@outlook.com

[![](https://static.igem.org/mediawiki/2020/9/99/T--ECUST_China--031.png)](https://www.facebook.com/ecust.ecust)
[![](https://static.igem.org/mediawiki/2020/5/57/T--ECUST_China--01.png)](https://space.bilibili.com/633958833?share_medium=android&share_source=copy_link&bbid=XY7D24B7735A414A5DC4AB35DD72D2DE08FD4&ts=1603379493518)
[![](https://static.igem.org/mediawiki/2020/b/b2/T--ECUST_China--021.png)](https://mp.weixin.qq.com/s/b43sFIRw-VrWO82urP8zPA)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:ECUST China/Results

home

[![HOME](https://static.igem.org/mediawiki/2020/a/a7/T--ECUST_China--logo_chang.png)](https://2020.igem.org/Team%3AECUST_China)

#### [Team](https://2020.igem.org/Team%3AECUST_China/Members)

###### [Members](https://2020.igem.org/Team%3AECUST_China/Members)

###### [Collaborations](https://2020.igem.org/Team%3AECUST_China/Collaborations)

#### [Project](https://2020.igem.org/Team%3AECUST_China/Description)

###### [Description](https://2020.igem.org/Team%3AECUST_China/Description)

###### [Design](https://2020.igem.org/Team%3AECUST_China/Design)

###### [Experiments](https://2020.igem.org/Team%3AECUST_China/Experiments)

###### [Contribution](https://2020.igem.org/Team%3AECUST_China/Contribution)

###### [Attribution](https://2020.igem.org/Team%3AECUST_China/Attributions)

###### [Safety](https://2020.igem.org/Team%3AECUST_China/Safety)

#### [Results](https://2020.igem.org/Team%3AECUST_China/Results)

###### [Engineering](https://2020.igem.org/Team%3AECUST_China/Engineering)

###### [Demonstrate](https://2020.igem.org/Team%3AECUST_China/Demonstrate)

###### [Improve](https://2020.igem.org/Team%3AECUST_China/Improve)

#### [Parts](https://2020.igem.org/Team%3AECUST_China/Parts)

#### [Human](https://2020.igem.org/Team%3AECUST_China/Human%20Practices)

###### [Human Practices](https://2020.igem.org/Team%3AECUST_China/Human%20Practices)

###### [Education](https://2020.igem.org/Team%3AECUST_China/Education)

#### [Awards](https://2020.igem.org/Team%3AECUST_China/Awards)

###### [Hardware](https://2020.igem.org/Team%3AECUST_China/Hardware)

###### [Model](https://2020.igem.org/Team%3AECUST_China/Model)

###### [Implementation](https://2020.igem.org/Team%3AECUST_China/Implementation)

###### [Proof of concept](https://2020.igem.org/Team%3AECUST_China/Proof_Of_Concept)

![](https://static.igem.org/mediawiki/2020/b/bf/T--ECUST_China--results.png)

Achievements

![](https://static.igem.org/mediawiki/2020/f/f1/T--ECUST_China--results_tick.jpg)

1.established a digital microfluidics system rooted in electrowetting on dielectric (EWOD) for the fast and accurate COVID-19 test

![](https://static.igem.org/mediawiki/2020/f/f1/T--ECUST_China--results_tick.jpg)

2.improved reaction environment so as to preform DNA Walker and E-CRISPR sequentially

![](https://static.igem.org/mediawiki/2020/f/f1/T--ECUST_China--results_tick.jpg)

3.enhanced sequence design for the DNA Walker aiming at an improved limit of detection

![](https://static.igem.org/mediawiki/2020/f/f1/T--ECUST_China--results_tick.jpg)

4.reworked E-CRISPR system in order to significantly amplify the digital signal

![](https://static.igem.org/mediawiki/2020/f/f1/T--ECUST_China--results_tick.jpg)

5.developed a Mini Program connected to the testing device so that the users could know the results instantly

![](https://static.igem.org/mediawiki/2020/f/f1/T--ECUST_China--results_tick.jpg)

6.established a simulation to predict the droplet flow in the digital microfluidics system

![](https://static.igem.org/mediawiki/2020/f/f1/T--ECUST_China--results_tick.jpg)

7.improved method of extracting RNA sample from the swabs

#### ECUST\_China

EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Shanghai, China

[![](https://static.igem.org/mediawiki/2020/9/97/T--ECUST_China--ECUST_logo.png)](https://www.ecust.edu.cn/)
[![](https://static.igem.org/mediawiki/2020/7/79/T--ECUST_China--%E7%94%9F%E5%B7%A5-logo.jpg)](http://biotech.ecust.edu.cn/)

#### GET IN TOUCH

+86 17553856936

iGEM\_ECUST@outlook.com

[![](https://static.igem.org/mediawiki/2020/9/99/T--ECUST_China--031.png)](https://www.facebook.com/ecust.ecust)
[![](https://static.igem.org/mediawiki/2020/5/57/T--ECUST_China--01.png)](https://space.bilibili.com/633958833?share_medium=android&share_source=copy_link&bbid=XY7D24B7735A414A5DC4AB35DD72D2DE08FD4&ts=1603379493518)
[![](https://static.igem.org/mediawiki/2020/b/b2/T--ECUST_China--021.png)](https://mp.weixin.qq.com/s/b43sFIRw-VrWO82urP8zPA)