iGEM 2019 — Team SoundBio

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:SoundBio/Notebook

[![](https://static.igem.org/mediawiki/2019/thumb/c/cf/T--SoundBio--PlasmidLogo.png/1158px-T--SoundBio--PlasmidLogo.png)](https://2019.igem.org/Team%3ASoundBio)

[Team](https://2019.igem.org/Team%3ASoundBio/Team)

Project

[Description](https://2019.igem.org/Team%3ASoundBio/Project_Description)
[Background](https://2019.igem.org/Team%3ASoundBio/Background)
[Design](https://2019.igem.org/Team%3ASoundBio/Design)
[Experiments](https://2019.igem.org/Team%3ASoundBio/Experiments)
[Lab Notebook](https://2019.igem.org/Team%3ASoundBio/Lab_Notebook)
[Results](https://2019.igem.org/Team%3ASoundBio/Project/Results)
[Characterization](https://2019.igem.org/Team%3ASoundBio/Characterization)
[Safety](https://2019.igem.org/Team%3ASoundBio/Safety)
[Attributions](https://2019.igem.org/Team%3ASoundBio/Attributions)

Hardware

[Demonstration](https://2019.igem.org/Team%3ASoundBio/Demonstrate)

[Modeling](https://2019.igem.org/Team%3ASoundBio/Model)

Human Practices

[Integrated Human Practices](https://2019.igem.org/Team%3ASoundBio/Integrated_HP)
[Public Engagement](https://2019.igem.org/Team%3ASoundBio/Public_Engagement)

[Minterlab](https://2019.igem.org/Team%3ASoundBio/Minterlab)
[Collaborations](https://2019.igem.org/Team%3ASoundBio/Collaborations)

Awards

[Judging Form](https://igem.org/2019_Judging_Form?id=3012)

![](https://static.igem.org/mediawiki/2019/b/b8/T--soundbio--email.png)
[![](https://static.igem.org/mediawiki/2019/4/47/T--soundbio--facebook512.png)](https://www.facebook.com/soundbioigem/)
[![](https://static.igem.org/mediawiki/2019/b/ba/T--Purdue--instagram_black.png)](https://www.instagram.com/soundbioigem/)
[SoundBio iGEM](https://sites.google.com/view/soundbioigem)

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)


[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:SoundBio/Experiments

[![](https://static.igem.org/mediawiki/2019/thumb/c/cf/T--SoundBio--PlasmidLogo.png/1158px-T--SoundBio--PlasmidLogo.png)](https://2019.igem.org/Team%3ASoundBio)

[Team](https://2019.igem.org/Team%3ASoundBio/Team)

Project

[Description](https://2019.igem.org/Team%3ASoundBio/Project_Description)
[Background](https://2019.igem.org/Team%3ASoundBio/Background)
[Design](https://2019.igem.org/Team%3ASoundBio/Design)
[Experiments](https://2019.igem.org/Team%3ASoundBio/Experiments)
[Lab Notebook](https://2019.igem.org/Team%3ASoundBio/Lab_Notebook)
[Results](https://2019.igem.org/Team%3ASoundBio/Project/Results)
[Characterization](https://2019.igem.org/Team%3ASoundBio/Characterization)
[Safety](https://2019.igem.org/Team%3ASoundBio/Safety)
[Attributions](https://2019.igem.org/Team%3ASoundBio/Attributions)

Hardware

[Demonstration](https://2019.igem.org/Team%3ASoundBio/Demonstrate)

[Modeling](https://2019.igem.org/Team%3ASoundBio/Model)

Human Practices

[Integrated Human Practices](https://2019.igem.org/Team%3ASoundBio/Integrated_HP)
[Public Engagement](https://2019.igem.org/Team%3ASoundBio/Public_Engagement)

[Minterlab](https://2019.igem.org/Team%3ASoundBio/Minterlab)
[Collaborations](https://2019.igem.org/Team%3ASoundBio/Collaborations)

Awards

[Judging Form](https://igem.org/2019_Judging_Form?id=3012)

![](https://static.igem.org/mediawiki/2019/b/b8/T--soundbio--email.png)
[![](https://static.igem.org/mediawiki/2019/4/47/T--soundbio--facebook512.png)](https://www.facebook.com/soundbioigem/)
[![](https://static.igem.org/mediawiki/2019/b/ba/T--Purdue--instagram_black.png)](https://www.instagram.com/soundbioigem/)
[SoundBio iGEM](https://sites.google.com/view/soundbioigem)

![](https://static.igem.org/mediawiki/2019/4/42/T--SoundBio--ExperimentsHeader.png)

Experiments

[Protocols and Experiments](#PE)

## Protocols and Experiments

 [Bacterial Growth and Light Exposure Protocol](https://benchling.com/soundbio-igem/f/lib_r0W3EcHt-software/prt_shdjkULE-bacterial-growth-and-light-exposure-protocol/edit)

 [Creating Yamanaka Agar and Streaking It with K. rhaeticus](https://benchling.com/soundbio-igem/f/lib_bH07Bckj-2019-igem/prt_Mw5i5RU3-creating-yamanaka-agar-and-streaking-it-with-k-rhaeticus/edit)

 [DNA Fragment Sample Preparation for Genewiz Sanger Sequencing](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_5vX4BVvx-dna-fragment-sample-preparation-for-genewiz-sanger-sequencing/edit)

 [iGEM Distribution Kit Plate Instruction](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_MDeMuy1X-igem-distribution-kit-plate-instruction/edit)

 [K. rhaeticus HS Media and Culturing](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_5nvRooek-k-rhaeticus-hs-media-and-culturing/edit)

 [LB Media Recipe](https://benchling.com/soundbio-igem/f/lib_eDUgXLzb-media-recipes/prt_m78OmTss-lb-media-recipe/edit)

 [Ligation Protocol with T4 DNA Ligase (M0202)](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_P00RtbV4-ligation-protocol-with-t4-dna-ligase-m0202/edit)

 [Making and Running an Analytical Gel Electrophoresis](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_l7HVngLd-making-running-an-analytical-gel-electrophoresis/edit)

 [Making K. rhaeticus Stock from Cryostock](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_bD6XUARN-making-k-rhaeticus-stock-from-cryostock/edit)

[Making HS and Culturing Media](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_5nvRooek-k-rhaeticus-hs-media-and-culturing/edit)

 [Measuring dsDNA Concentration using the Nanodrop](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_u9OknWM8-measuring-dsdna-concentration-using-the-nanodrop/edit)

 [Miniprep Plasmid DNA Purification from E. Coli](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_IDF4YGPN-miniprep-plasmid-dna-purification-from-e-coli/edit)

 [PBS Solution](https://benchling.com/soundbio-igem/f/lib_r0W3EcHt-software/prt_H2S31Fef-pbs-solution/edit)

 [PCR Amplification of Plasmid DNA (Phusion)](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_PrVtOcCc-pcr-amplification-ofplasmid-dna-phusion/edit)

 [PCR Purification using Silica-Membrane-Based Column Purification](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_y3ZUfbqS-pcr-purification-using-silica-membrane-based-column-purification/edit)

 [Preparing Electrocompetent K. rhaeticus Cells](https://benchling.com/soundbio-igem/f/lib_eDUgXLzb-media-recipes/prt_KtgR0274-preparing-csl-media/edit%3E%20Preparing%20CSL%20Media%3C/a%3E%3Cbr%3E%3Ca%20href%3D)

 [Preparing Glycerol Stocks of K. rhaeticus](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_Kms3Ou38-preparing-glycerol-stocks-of-k-rhaeticus/edit)

 [Preparation of TSS Competent Cells](https://benchling.com/soundbio-igem/f/lib_DeE20NfY-tss/prt_neUT9zdv-preparation-of-tss-competent-cells/edit)

 [Preparation of TSS Solution](https://benchling.com/soundbio-igem/f/lib_DeE20NfY-tss/prt_OYxeLyTo-preparation-of-tss-solution/edit)

 [Qiagen Gel Extraction](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_g5WftyAi-qiagen-gel-extraction/edit)

 [Single-temperature Double Digest](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_DmTFblQ6-single-temperature-double-digest/edit)

 [SOB and SOC Media Recipe](https://benchling.com/soundbio-igem/f/lib_eDUgXLzb-media-recipes/prt_D7nZkxpG-sob-soc-media-recipe/edit)

 [Storing Organisms in the -80C Freezer](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_DMB0h3kc-storing-organisms-in-the-80c-freezer-igem/edit)

 [Transformation of K. rhaeticus via Electroporation](https://benchling.com/soundbio-igem/f/lib_QFiJZhgq-protocols/prt_HG7iTowz-transformation-of-k-rhaeticus-via-electroporation/edit)

 [TSS Chemical Transformation](https://benchling.com/soundbio-igem/f/lib_DeE20NfY-tss/prt_aD46WSbI-tss-chemical-transformation/edit)

 [-80 Preculture Aliquot Protocol](https://benchling.com/soundbio-igem/f/lib_r0W3EcHt-software/prt_nOD5HZ3k--80-preculture-aliquot-protocol/edit)

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:SoundBio/Results

[![](https://static.igem.org/mediawiki/2019/thumb/c/cf/T--SoundBio--PlasmidLogo.png/1158px-T--SoundBio--PlasmidLogo.png)](https://2019.igem.org/Team%3ASoundBio)

[Team](https://2019.igem.org/Team%3ASoundBio/Team)

Project

[Description](https://2019.igem.org/Team%3ASoundBio/Project_Description)
[Background](https://2019.igem.org/Team%3ASoundBio/Background)
[Design](https://2019.igem.org/Team%3ASoundBio/Design)
[Experiments](https://2019.igem.org/Team%3ASoundBio/Experiments)
[Lab Notebook](https://2019.igem.org/Team%3ASoundBio/Lab_Notebook)
[Results](https://2019.igem.org/Team%3ASoundBio/Project/Results)
[Characterization](https://2019.igem.org/Team%3ASoundBio/Characterization)
[Safety](https://2019.igem.org/Team%3ASoundBio/Safety)
[Attributions](https://2019.igem.org/Team%3ASoundBio/Attributions)

Hardware

[Demonstration](https://2019.igem.org/Team%3ASoundBio/Demonstrate)

[Modeling](https://2019.igem.org/Team%3ASoundBio/Model)

Human Practices

[Integrated Human Practices](https://2019.igem.org/Team%3ASoundBio/Integrated_HP)
[Public Engagement](https://2019.igem.org/Team%3ASoundBio/Public_Engagement)

[Minterlab](https://2019.igem.org/Team%3ASoundBio/Minterlab)
[Collaborations](https://2019.igem.org/Team%3ASoundBio/Collaborations)

Awards

[Judging Form](https://igem.org/2019_Judging_Form?id=3012)

![](https://static.igem.org/mediawiki/2019/b/b8/T--soundbio--email.png)
[![](https://static.igem.org/mediawiki/2019/4/47/T--soundbio--facebook512.png)](https://www.facebook.com/soundbioigem/)
[![](https://static.igem.org/mediawiki/2019/b/ba/T--Purdue--instagram_black.png)](https://www.instagram.com/soundbioigem/)
[SoundBio iGEM](https://sites.google.com/view/soundbioigem)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the part's Main Page on the Registry.** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)