iGEM 2018 — Team NAWI_Graz

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# Team:NAWI Graz/Notebook

![](https://static.igem.org/mediawiki/2018/d/da/T--NAWI_Graz--Notebook_Title.png)

* [![](https://static.igem.org/mediawiki/2018/6/6c/T--NAWI_Graz--icon_project.svg)
  Project](https://2018.igem.org/Team%3ANAWI_Graz)

  [Home](https://2018.igem.org/Team%3ANAWI_Graz)[Description](https://2018.igem.org/Team%3ANAWI_Graz/Description)
  [Design](https://2018.igem.org/Team%3ANAWI_Graz/Design)
* ![](https://static.igem.org/mediawiki/2018/4/49/T--NAWI_Graz--icon_flask2.svg)
  Lab

  [Protocols](https://2018.igem.org/Team%3ANAWI_Graz/Protocols)
  [Notebook](https://2018.igem.org/Team%3ANAWI_Graz/Notebook)
  [Improve](https://2018.igem.org/Team%3ANAWI_Graz/Improve)
  [InterLab](https://2018.igem.org/Team%3ANAWI_Graz/InterLab)
* ![](https://static.igem.org/mediawiki/2018/a/aa/T--NAWI_Graz--icon_globe2.svg)
  Human Practices

  [Human Practices](https://2018.igem.org/Team%3ANAWI_Graz/Human_Practices)
  [Public Engagement](https://2018.igem.org/Team%3ANAWI_Graz/Public_Engagement)
* ![](https://static.igem.org/mediawiki/2018/f/f6/T--NAWI_Graz--icon_team2.svg)
  Team

  [Team Members](https://2018.igem.org/Team%3ANAWI_Graz/Team)
  [Attributions](https://2018.igem.org/Team%3ANAWI_Graz/Attributions)
  [Collaborations](https://2018.igem.org/Team%3ANAWI_Graz/Collaborations)

### October

* Team assembly with guidance from the previous team

* creation of a facebook site and exchange of contact data

* setting up of a weekly meet up appointment

###### 23.10.2017

first introduction to the competition and team assembly

###### 30.10.2017

second meet up with members of the 2017 team; contact data exchange; creation of various communication platforms and setting up of a weekly meet-up appointment;

### November

* start of brainstorming sessions to find the project we wanted to realize
* first deliberations about funding of the project
* first contacts about lab and office space

### December

* team members with already assigned tasks left the team because of university obligations or personal reasons, sizing us down to 6 people from previously 18
* still ongoing project finding

### January

* asking people in the respective fields about the viability of our collected project ideas
* deciding on the palm kernel oil project since it is such a huge discussion point in austria

### February

* creation of a budget plan (Anna, Marlene)
* presentation of our project idea to the deanery of the universities TU-Graz and KFU-Graz

### March

* search for a lab space for the preliminary tests (Thomas)
* extensive change to the project design (esterification)
* gain of 3 new members raising our number to 9

###### 21.3.2018

Movie night with following presentation of our project at the university (Anna, Marlene, Marco, Henrik, Veronika, Thomas)

### April

* improved budget plan
* planing of the new preliminary tests and laboratory allocation (Marco, Dominik, Thomas)
* igem-graz.at was created and set (Jakob)

###### 13.4.2018

lange nacht der forschung - information evening for visitors of the universities or research facilities

###### 28.4.2018

Teambuilding evening - the team got together for a game of escape the room

### May

* preliminary yeast tests
* first two weeks: WT and elo knock out strain
* rest of the month: checking the extracts of other strains for better compatibility.(Marco, Dominik, Thomas)

###### 7.5.2018

first day of the preliminary tests

###### 30.5.2018

Mol-Bio Fest'l - annually public event at the institut to gain more attention for our project and the iGEM competition and celebrate the achievements

### June

* Labwork came to a halt as a result of other obligations at the university since this is a busy time (labcourses and exams)
* contacting a lot of companies for possible cooperations (Anna, Marlene, Veronika, Birgit)

###### 8.6.2018

Teambuilding evening

###### 16.6.2018

TU-Graz StreeTech - public outreach event in front of the city hall Graz to present all student competition teams and projects of the TU Graz including the iGEM teams NAWI\_Graz 2017 and 2018 (Anna, Marlene, Henrik, Veronika, Thomas)

###### 26.6.2018

BHAK Weiz - public outreach in form of a presentation and small DNA extraction experiments in a public school to gain more attention for our field of study and iGEM itself (Anna, Marlene, Veronika)

### July

* creation of the polycistronic expression cassette in snapgene, to be implemented with CRISPR-Cas9 and lambda red recombineering into *E. coli* K12 MG1655 at the position of the *dgkA* gene – knocking it out in the process. The creation took some time since the correct use of snapgene had to be learned and several meetings with professionals from ACIB where needed. They explained to us the correct way to create an expression casette for our chosen system (two-vector system proposed by Jiang et.al. In 2015 ) and the most common errors that could occur. (Thomas, Dominik)

###### 5.7.2018

Project presentation at Biomin - a local animal nutirion company (Marlene, Birgit, Thomas)

###### 20.7.2018

iGEM meetup munich

###### 21.7.2018

iGEM meetup munich

###### 22.7.2018

iGEM meetup munich

### August/September/October

* Main Labwork (Marco, Dominik, Thomas)
* Programming/Design of the Wiki Page (Jakob)

###### 24.8.2018

OE-PCR of gblocks 1+2, 3+4, 5+6, 7+8, 9+10 with limited success

###### 27.8.2018

OE-PCR of only the first 2 gblocks under different conditions to improve the yield of combined gblocks.

###### 28.8.2018

OE-PCR reaction of the first set of gblocks with a diffrent protocol that was suggested by professionals at ACIB; agarose gel electrophoresis to check the results of our PCR;

###### 29.8.2018

innoculation of YPD medium with delta-desaturase pre-culture; cutting the gblock bands from our agarose gel and storing them in freezer;

###### 30.8.2018

yeast batch was not grown enough and kept in the shaker

###### 31.8.2018

yeast batch was still at a low cell count and incubated for another day

###### 3.9.2018

determination of the cell count and microscopy of the delta desaturase strain to check on the condition of the cells; harvest of the cells and freezing them with liquid nitrogen for storage in a -80°C freezer till lipidextraction is in order.

###### 4.9.2018

plating of the plasmid carrying bacteria on agarose plates for the creation of a glycerol stock for long term storage.

###### 5.9.2018

restricition digest of plasmid backbone psB1K3 and the gblocks *tesA* mutated C12/C14, *atfA* old and *atfA* optimized;

###### 6.9.2018

inactivation of restriction enzymes; ligation of the gblocks into the psB1K3 backbone

###### 7.9.2018

gel purification of the stored agarose bands; desalting of the ligation mix; colony PCR for amplification of the weak bands from the gel;

###### 9.9.2018

OE-PCR tesA, gblock1-2, gblock3-4, gblock9-10

###### 10.9.2018

agarose gel purification of *tesA* and 1+2, 3+4; nanodrop measurement

###### 11.9.2018

OE-pcr gblocks 1+2, 3+4, 7+8 ;

###### 12.9.2018

1+2+3+4 oe-pcr and gelpurification

###### 13.9.2018

Interlab Marburg begin

###### 14.9.2018

preparation for PCR and two vector system

###### 15.9.2018

cPCR

###### 16.9.2018

Agarose gel electrophoresis, gel purification

###### 17.9.2018

PCR (different thermocycler), electrophoresis, purification

###### 18.9.2018

Nanodrop, PCR (better thermocycler again), electrophoresis, purification Nanodrop

###### 19.9.2018

restriction digest, ligation, preparation of spec and spec/kan media ONC of plasmid stocks

###### 20.9.2018

miniprep, double restriction digest, ligation, gel elctrophoresis, single digest

###### 21.9.2018

gel electrophoresis, double digest (longer time)?

###### 22.9.2018

tranformation, double restriction digest, ligation, miniprep, Yale conference

###### 23.9.2018

cultivation of electrocompetent cells, transforamation, miniprep, ligation

###### 24.9.2018

ONCs, gel elctrophoresis

###### 25.9.2018

minipreps, gel electrophoresis, gel purification, PCR

###### 26.9.2018

PCR, restriction digest, ligation, gel elctrophoresis

###### 27.9.2018

PCR, gel-electrophoresis, transformation, ONCs, TU-welcome days - a public outreach event for graduates to find their future studys

###### 28.9.2018

single colony streaks, ONCs, (successful biobrick implementation), TU-welcome days

###### 29.9.2018

miniprep; getting biobricks ready for transport

###### 10.10.2018

Spritzerstand'l - a typical public celebration at our university for especially students and our form to prepare for the final project part, the "horror"-wiki week

###### 11.10.2018

“Dirndl und Lederhosen” photoshoot - a local seller of traditional clothing helped us to produce our final team pictures and clearly represent our (educationally) homeland Styria as a part of Austria
ongoing work on the wiki page

###### 16.10.2018

ongoing wotk on the wiki page
first events for the new team assembly

###### 17.10.2018

finalisation of the wiki page
heading of to vienna and checking in for our flight to Boston - see you all there!

### end of log

---

![](https://static.igem.org/mediawiki/2018/e/ef/T--NAWI_Graz--allSp_Sponsor.png)


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# Team:NAWI Graz/Experiments

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[HOME](https://2018.igem.org/Team%3ANAWI_Graz)

TEAM

[Team Members](https://2018.igem.org/Team%3ANAWI_Graz/Team)
[Collaborations](https://2018.igem.org/Team%3ANAWI_Graz/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ANAWI_Graz/Description)
[Design](https://2018.igem.org/Team%3ANAWI_Graz/Design)
[Experiments](https://2018.igem.org/Team%3ANAWI_Graz/Experiments)
[Notebook](https://2018.igem.org/Team%3ANAWI_Graz/Notebook)
[InterLab](https://2018.igem.org/Team%3ANAWI_Graz/InterLab)
[Model](https://2018.igem.org/Team%3ANAWI_Graz/Model)
[Results](https://2018.igem.org/Team%3ANAWI_Graz/Results)
[Demonstrate](https://2018.igem.org/Team%3ANAWI_Graz/Demonstrate)
[Improve](https://2018.igem.org/Team%3ANAWI_Graz/Improve)
[Attributions](https://2018.igem.org/Team%3ANAWI_Graz/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ANAWI_Graz/Parts)
[Basic Parts](https://2018.igem.org/Team%3ANAWI_Graz/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ANAWI_Graz/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ANAWI_Graz/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ANAWI_Graz/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ANAWI_Graz/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ANAWI_Graz/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ANAWI_Graz/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ANAWI_Graz/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ANAWI_Graz/Hardware)
[Measurement](https://2018.igem.org/Team%3ANAWI_Graz/Measurement)
[Model](https://2018.igem.org/Team%3ANAWI_Graz/Model)
[Plant](https://2018.igem.org/Team%3ANAWI_Graz/Plant)
[Software](https://2018.igem.org/Team%3ANAWI_Graz/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=NAWI_Graz)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:NAWI Graz/Results

![](https://static.igem.org/mediawiki/2018/4/4f/2018_igem_mobile_menu_logo.svg)

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[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3ANAWI_Graz)
[HOME](https://2018.igem.org/Team%3ANAWI_Graz)

TEAM

[Team Members](https://2018.igem.org/Team%3ANAWI_Graz/Team)
[Collaborations](https://2018.igem.org/Team%3ANAWI_Graz/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ANAWI_Graz/Description)
[Design](https://2018.igem.org/Team%3ANAWI_Graz/Design)
[Experiments](https://2018.igem.org/Team%3ANAWI_Graz/Experiments)
[Notebook](https://2018.igem.org/Team%3ANAWI_Graz/Notebook)
[InterLab](https://2018.igem.org/Team%3ANAWI_Graz/InterLab)
[Model](https://2018.igem.org/Team%3ANAWI_Graz/Model)
[Results](https://2018.igem.org/Team%3ANAWI_Graz/Results)
[Demonstrate](https://2018.igem.org/Team%3ANAWI_Graz/Demonstrate)
[Improve](https://2018.igem.org/Team%3ANAWI_Graz/Improve)
[Attributions](https://2018.igem.org/Team%3ANAWI_Graz/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ANAWI_Graz/Parts)
[Basic Parts](https://2018.igem.org/Team%3ANAWI_Graz/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ANAWI_Graz/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ANAWI_Graz/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ANAWI_Graz/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ANAWI_Graz/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ANAWI_Graz/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ANAWI_Graz/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ANAWI_Graz/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ANAWI_Graz/Hardware)
[Measurement](https://2018.igem.org/Team%3ANAWI_Graz/Measurement)
[Model](https://2018.igem.org/Team%3ANAWI_Graz/Model)
[Plant](https://2018.igem.org/Team%3ANAWI_Graz/Plant)
[Software](https://2018.igem.org/Team%3ANAWI_Graz/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=NAWI_Graz)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)