iGEM 2010 — Team LMU-Munich

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# Team:LMU-Munich/Notebook

### From 2010.igem.org

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| [![](http://igem.semicolonsoftware.de/mediawiki/images/2/27/IGEMLMU.jpg)](/Team%3ALMU-Munich) | ![](http://igem.semicolonsoftware.de/mediawiki/images/1/1f/Muenchen.jpg) |
| [![](http://igem.semicolonsoftware.de/mediawiki/images/5/5b/Homenew.gif)](/Team%3ALMU-Munich)[![](http://igem.semicolonsoftware.de/mediawiki/images/5/55/Teamnew.gif)](/Team%3ALMU-Munich/Team)[![](http://igem.semicolonsoftware.de/mediawiki/images/d/de/P1new.gif)](/Team%3ALMU-Munich/ApoControl)[![](http://igem.semicolonsoftware.de/mediawiki/images/9/9c/HPnew.gif)](/Team%3ALMU-Munich/HumanPractice)[![](http://igem.semicolonsoftware.de/mediawiki/images/6/63/NOTEBOOKnew.gif)](/Team%3ALMU-Munich/Notebook)[![](http://igem.semicolonsoftware.de/mediawiki/images/d/d2/Safetynew.gif)](/Team%3ALMU-Munich/Safety)[![](http://igem.semicolonsoftware.de/mediawiki/images/8/86/Partanew.gif)](/Team%3ALMU-Munich/Parts)[![](http://igem.semicolonsoftware.de/mediawiki/images/1/17/Sponsoring-Topnew.gif)](/Team%3ALMU-Munich/Sponsoring)[![](http://igem.semicolonsoftware.de/mediawiki/images/4/4e/Loginnew.gif)](https://2010.igem.org/Team%3ALMU-Munich/Azo_Switch/crosslinks/Picture_gallery)![](http://igem.semicolonsoftware.de/mediawiki/images/3/31/RechtsTop.jpg) |

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| |  |  | | --- | --- | | ![](http://igem.semicolonsoftware.de/mediawiki/images/b/bd/LeftBorder.jpg) | [![](http://igem.semicolonsoftware.de/mediawiki/images/7/7a/LeftAponew3.gif)](/Team%3ALMU-Munich/ApoControl) | |  | |

* **[Cut'N'Survive System](/Team%3ALMU-Munich/Cut%27N%27survive "Team:LMU-Munich/Cut'N'survive")**
  + [Functional Principle](/Team%3ALMU-Munich/Cut%27N%27survive/Functional_Principle "Team:LMU-Munich/Cut'N'survive/Functional Principle")
  + [Sequences](/Team%3ALMU-Munich/Cut%27N%27survive/Sequences "Team:LMU-Munich/Cut'N'survive/Sequences")
  + [Schedule](/Team%3ALMU-Munich/Cut%27N%27survive/Schedule "Team:LMU-Munich/Cut'N'survive/Schedule")
* **[Jump-or-Die System](/Team%3ALMU-Munich/Jump-or-die "Team:LMU-Munich/Jump-or-die")**
  + [Functional Principle](/Team%3ALMU-Munich/Jump-or-die/Functional_Principle "Team:LMU-Munich/Jump-or-die/Functional Principle")
  + [Sequences](/Team%3ALMU-Munich/Jump-or-die/Seqences "Team:LMU-Munich/Jump-or-die/Seqences")
  + [Schedule](/Team%3ALMU-Munich/Jump-or-die/Schedule "Team:LMU-Munich/Jump-or-die/Schedule")
* **[ProSearch System](/Team%3ALMU-Munich/ProSearch "Team:LMU-Munich/ProSearch")**
  + [Schedule](/Team%3ALMU-Munich/ProSearch/Schedule "Team:LMU-Munich/ProSearch/Schedule")

| [![](http://igem.semicolonsoftware.de/mediawiki/images/8/88/LeftPathnew3.gif)](/Team%3ALMU-Munich/Pathway) |
| * [Functional Principle](/Team%3ALMU-Munich/Functional_Principle "Team:LMU-Munich/Functional Principle") * [Sequences](/Team%3ALMU-Munich/Sequences "Team:LMU-Munich/Sequences") * [Schedule](/Team%3ALMU-Munich/Schedule "Team:LMU-Munich/Schedule")      * **[picture gallery](/Team%3ALMU-Munich/Azo_Switch/crosslinks/Picture_gallery "Team:LMU-Munich/Azo Switch/crosslinks/Picture gallery")** |
 |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | | **Notebook** You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.   |  | [**ApoControl**](/Team%3ALMU-Munich/Notebook/Apoptosis "Team:LMU-Munich/Notebook/Apoptosis") | [**Pathway**](/Team%3ALMU-Munich/Notebook/Pathway "Team:LMU-Munich/Notebook/Pathway") | | --- | --- | --- |  **Protocols**  * [1 competent cells](/Team%3ALMU-Munich/Notebook/Protocols/1_Competent_cells "Team:LMU-Munich/Notebook/Protocols/1 Competent cells") * [2 highly competent cells](/Team%3ALMU-Munich/Notebook/Protocols/2_Highly_competent_cells "Team:LMU-Munich/Notebook/Protocols/2 Highly competent cells") * [3 Transformation](/Team%3ALMU-Munich/Notebook/Protocols/3_Transformation "Team:LMU-Munich/Notebook/Protocols/3 Transformation") * [4 Plasmid extraction from cells](/Team%3ALMU-Munich/Notebook/Protocols/4_Plasmid_extraction_from_cells "Team:LMU-Munich/Notebook/Protocols/4 Plasmid extraction from cells") * [5 Restriction digestion](/Team%3ALMU-Munich/Notebook/Protocols/5_Restriction_digest "Team:LMU-Munich/Notebook/Protocols/5 Restriction digest") * [6 SOB medium](/Team%3ALMU-Munich/Notebook/Protocols/6_SOB_medium "Team:LMU-Munich/Notebook/Protocols/6 SOB medium") * [7 Measurement of competence](/Team%3ALMU-Munich/Notebook/Protocols/7_Measurement_of_competence "Team:LMU-Munich/Notebook/Protocols/7 Measurement of competence") * [8 SOC medium](/Team%3ALMU-Munich/Notebook/Protocols/8_SOC_medium "Team:LMU-Munich/Notebook/Protocols/8 SOC medium") * [9 Handling primers](/Team%3ALMU-Munich/Notebook/Protocols/9_Handling_primers "Team:LMU-Munich/Notebook/Protocols/9 Handling primers") * [10 PCR with Pfu](/Team%3ALMU-Munich/Notebook/Protocols/10_PCR_with_Pfu "Team:LMU-Munich/Notebook/Protocols/10 PCR with Pfu") * [11 Agarose gel electrophoresis](/Team%3ALMU-Munich/Notebook/Protocols/11_Agarose_gel_electrophoresis "Team:LMU-Munich/Notebook/Protocols/11 Agarose gel electrophoresis") * [12 Gel extraction or PCR Clean up (Promega kit)](/Team%3ALMU-Munich/Notebook/Protocols/12_Gel_extraction_or_PCR_Clean_up "Team:LMU-Munich/Notebook/Protocols/12 Gel extraction or PCR Clean up") * [13 3A Method for Biobrick assembly](/Team%3ALMU-Munich/Notebook/Protocols/13_3A_Method_for_Biobrick_assembly "Team:LMU-Munich/Notebook/Protocols/13 3A Method for Biobrick assembly") * [14 QIAEX II gel extraction](/Team%3ALMU-Munich/Notebook/Protocols/14_QIAEX_II_gel_extraction "Team:LMU-Munich/Notebook/Protocols/14 QIAEX II gel extraction") * [15 PCR with Phusion](/Team%3ALMU-Munich/Notebook/Protocols/15_PCR_with_Phusion "Team:LMU-Munich/Notebook/Protocols/15 PCR with Phusion") * [16 PCR with DreamTaq](/Team%3ALMU-Munich/Notebook/Protocols/16_PCR_with_DreamTaq "Team:LMU-Munich/Notebook/Protocols/16 PCR with DreamTaq") * [17 Special thermal cycler programs](/Team%3ALMU-Munich/Notebook/Protocols/17_Special_thermal_cycler_programs "Team:LMU-Munich/Notebook/Protocols/17 Special thermal cycler programs") * [18 competent cells2](/Team%3ALMU-Munich/Notebook/Protocols/18_Competent_cells2 "Team:LMU-Munich/Notebook/Protocols/18 Competent cells2") * [19 Transformation SSS](/Team%3ALMU-Munich/Notebook/Protocols/19_Transformation_SSS "Team:LMU-Munich/Notebook/Protocols/19 Transformation SSS") * [20 PCR with Phusion Hot Start](/Team%3ALMU-Munich/Notebook/Protocols/20_PCR_with_Phusion_Hot_Start "Team:LMU-Munich/Notebook/Protocols/20 PCR with Phusion Hot Start") * [21 PCR with Taq Mastermix](/Team%3ALMU-Munich/Notebook/Protocols/21_PCR_with_Taq_Mastermix "Team:LMU-Munich/Notebook/Protocols/21 PCR with Taq Mastermix") * [22 Ligation](/Team%3ALMU-Munich/Notebook/Protocols/22_Ligation "Team:LMU-Munich/Notebook/Protocols/22 Ligation") * [23 LB Medium](/Team%3ALMU-Munich/Notebook/Protocols/23_LB_Medium "Team:LMU-Munich/Notebook/Protocols/23 LB Medium") * [24 Colony PCR](/Team%3ALMU-Munich/Notebook/Protocols/24_Colony_PCR "Team:LMU-Munich/Notebook/Protocols/24 Colony PCR") * [PCR with Taq](/Team%3ALMU-Munich/Notebook/Protocols/25_PCR_with_Taq "Team:LMU-Munich/Notebook/Protocols/25 PCR with Taq") | | ![](http://igem.semicolonsoftware.de/mediawiki/images/7/74/RightBorder.jpg) | | ![](http://igem.semicolonsoftware.de/mediawiki/images/9/97/LeftBottom.gif) | ![](http://igem.semicolonsoftware.de/mediawiki/images/f/fe/BottomLogo.gif) | |  | | |

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