iGEM 2016 — Team British_Columbia

[![](https://static.igem.org/mediawiki/2016/8/8b/HQ_page_logo.jpg)](https://2016.igem.org)

# Team:British Columbia/Notebook

Main CSS

Navbar CSS

* Project
  + [Description](https://2016.igem.org/Team%3ABritish_Columbia/Description)
  + S-Layer
    - [Cellulases](https://2016.igem.org/Team%3ABritish_Columbia/Project/S-Layer/Cellulases)
    - [Laccases](https://2016.igem.org/Team%3ABritish_Columbia/Project/S-Layer/Laccases)
  + [Biosynthetic Pathways](https://2016.igem.org/Team%3ABritish_Columbia/Project/Bio-Pathways)
  + [Consortia](https://2016.igem.org/Team%3ABritish_Columbia/Project/Consortia)
  + [Proof](https://2016.igem.org/Team%3ABritish_Columbia/Proof)
* [Modelling](https://2016.igem.org/Team%3ABritish_Columbia/Model)
* Human Practices
  + [Introduction](https://2016.igem.org/Team%3ABritish%20Columbia/HP/Gold)
  + [Infographic](https://2016.igem.org/Team%3ABritish_Columbia/Human_Practices/Infographic)
  + [Interview](https://2016.igem.org/Team%3ABritish_Columbia/Human_Practices/Interview)
* Team
  + [Bios](https://2016.igem.org/Team%3ABritish_Columbia/Team/Bios)
  + [Attributions](https://2016.igem.org/Team%3ABritish_Columbia/Attributions)
  + [Sponsors](https://2016.igem.org/Team%3ABritish_Columbia/Team/Sponsors)
* [Notebook](https://2016.igem.org/Team%3ABritish_Columbia/Notebook)
* Achievements
  + [Collaborations](https://2016.igem.org/Team%3ABritish_Columbia/Collaborations)
  + [Medal Criteria](https://2016.igem.org/Team%3ABritish_Columbia/Achievements/Medal_Criteria)
  + Parts
    - [Parts](https://2016.igem.org/Team%3ABritish_Columbia/Parts)
    - [Basic Part](https://2016.igem.org/Team%3ABritish_Columbia/Basic_Part)
    - [Composite Part](https://2016.igem.org/Team%3ABritish_Columbia/Composite_Part)
    - [Parts Collection](https://2016.igem.org/Team%3ABritish_Columbia/Part_Collection)

* Project
  + [Description](https://2016.igem.org/Team%3ABritish_Columbia/Description)
  + S-Layer
    - [Cellulases](https://2016.igem.org/Team%3ABritish_Columbia/Project/S-Layer/Cellulases)
    - [Laccases](https://2016.igem.org/Team%3ABritish_Columbia/Project/S-Layer/Laccases)
  + [Biosynthetic Pathways](https://2016.igem.org/Team%3ABritish_Columbia/Project/Bio-Pathways)
  + [Consortia](https://2016.igem.org/Team%3ABritish_Columbia/Project/Consortia)
  + [Proof](https://2016.igem.org/Team%3ABritish_Columbia/Proof)
* [Modelling](https://2016.igem.org/Team%3ABritish_Columbia/Model)
* Human Practices
  + [Introduction](https://2016.igem.org/Team%3ABritish%20Columbia/HP/Gold)
  + [Infographic](https://2016.igem.org/Team%3ABritish_Columbia/Human_Practices/Infographic)
  + [Interview](https://2016.igem.org/Team%3ABritish_Columbia/Human_Practices/Interview)
* Team
  + [Bios](https://2016.igem.org/Team%3ABritish_Columbia/Team/Bios)
  + [Attributions](https://2016.igem.org/Team%3ABritish_Columbia/Attributions)
  + [Sponsors](https://2016.igem.org/Team%3ABritish_Columbia/Team/Sponsors)
* [Notebook](https://2016.igem.org/Team%3ABritish_Columbia/Notebook)
* Achievements
  + [Collaborations](https://2016.igem.org/Team%3ABritish_Columbia/Collaborations)
  + [Medal Criteria](https://2016.igem.org/Team%3ABritish_Columbia/Achievements/Medal_Criteria)
  + Parts
    - [Parts](https://2016.igem.org/Team%3ABritish_Columbia/Parts)
    - [Basic Part](https://2016.igem.org/Team%3ABritish_Columbia/Basic_Part)
    - [Composite Part](https://2016.igem.org/Team%3ABritish_Columbia/Composite_Part)
    - [Parts Collection](https://2016.igem.org/Team%3ABritish_Columbia/Part_Collection)

Sidebar CSS

Notebook

**Notebook**

**[Home](https://2016.igem.org/Team%3ABritish_Columbia) /
[Notebook](https://2016.igem.org/Team%3ABritish_Columbia/Notebook)**

* [Beta Carotene Extraction](#1)
* [Caulobacter Electroporation Transformation](#2)
* [Chemically Competent E. coli Transformation](#3)
* [DNA Agarose Gel Electrophoresis](#4)
* [PCR Master Mix](#5)
* [PCR Amplification](#6)
* [Colony PCR DNA Amplification](#7)
* [Protocol for Restriction Enzyme Digest, Ligation, and Transformation](#8)
* [Western Blot](#9)

## Beta Carotene Extraction

## Caulobacter Electroporation Transformation

## Chemically Competent E. coli Transformation

## DNA Agarose Gel Electrophoresis

## PCR Master Mix

## PCR Amplification

## Colony PCR DNA Amplification

## Protocol for Restriction Enzyme Digest, Ligation, and Transformation

## Western Blot

**Check out other parts of our project below!**

[![](https://static.igem.org/mediawiki/2016/6/61/T--British_Columbia--header-lake-2.JPG)](https://2016.igem.org/Team%3ABritish_Columbia/Collaborations)
[**Collaborations**](https://2016.igem.org/Team%3ABritish_Columbia/Collaborations)

[![](https://static.igem.org/mediawiki/2016/e/ee/T--British_Columbia--header-tree.JPG)](https://2016.igem.org/Team%3ABritish_Columbia/Achievements/Medal_Criteria)
[**Medal Criteria**](https://2016.igem.org/Team%3ABritish_Columbia/Achievements/Medal_Criteria)

[![](https://static.igem.org/mediawiki/2016/b/bf/HQ_menu_logo.jpg)](https://2016.igem.org)