iGEM 2016 — Team Saint_Rose_School_B

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# Team:Saint Rose School B/Notebook

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* [Home](https://2016.igem.org/Team%3ASaint_Rose_School_B)
* [Project](https://2016.igem.org/Team%3ASaint_Rose_School_B/Description)
* [Notebook](https://2016.igem.org/Team%3ASaint_Rose_School_B/Notebook)
* [Results](https://2016.igem.org/Team%3ASaint_Rose_School_B/Results)
* [Safety](https://2016.igem.org/Team%3ASaint_Rose_School_B/Safety)
* [Human Practices](https://2016.igem.org/Team%3ASaint_Rose_School_B/Human_Practices)
* [Collaborations](https://2016.igem.org/Team%3ASaint_Rose_School_B/Collaborations)
* [Attributions](https://2016.igem.org/Team%3ASaint_Rose_School_B/Attributions)
* [Team](https://2016.igem.org/Team%3ASaint_Rose_School_B/Team)

# Notebook

# Journal:

- **June**

- **Thursday 2nd:**
- Scientific fair, Academia de Emprendimiento.

- **Saturday 25th:**
- We search for lignin degradation enzymes.

- **Wednesday 29th:**
- We investigate for more of these enzymes.

- **July**

- **Friday 1st:**
- We create our GMO.

- **Saturday 2nd:**
- We did cultivate bacteria with LuxI, LuxR and PLux.

- **Wednesday 6th:**
- We extract the plasmids of LuxR, LuxI and PLux from the E.coli bacteria and check it (electrophoresis).

- **Friday 8th:**
- We extract LiP and Lac from the plates and then we transform it.

- **Wednesday 13th:**
- We made again the cultivate with the plasmid extraction of LuxR, LuxI and PLux.
- We cultivate LiP and Lac.

- **Friday 15th:**
- Double digestion of LuxR, LuxI and PLux.
- Check the electrophoresis.

- **Friday 22nd:**
- We found the space where the genes (RFP and CFP) are, and we extract it from the plates and we transform it.

- **Saturday 23rd:**
- We extract the plasmid of LiP and Lac from the cultive, and then we did electrophoresis to check the size.

- **Friday 29th:**
- We cultiveted bacteria of RFP and CFP, and then electrophoresis to check the size of the plasmids.

- **August**

- **Wednesday 3rd:**
- We extract the plasmid of RFP y CFP.
- Presentation of groups progress.

- **Friday 5th:**
- We did double digestion of LiP and Lac, then we did electrophoresis to check the size.
- Check the information.

- **Saturday 6th:**
- We dedicated to read papers.
- We do some investigation and search.
- We check the result of the plasmidial extraction of CFP and RFP.

- **Wednesday 10th:**
- Check of information.
- Add ClbR an Cbh1 to our modeling design.
- We helped to others school in the check of extractions.
- Reading of papers.

- **Friday 12th:**
- Question to the team.
- Little rehearsal team.
- Investigation of ClbR an Cbh1 function.

- **Saturday 13th:**
- We practice genes ligation with others schools.

- **Saturday 20th:**
- Lab work (Valentina, Bastian e Isabela).
- Helped to the others groups in the lab (Natalia, Macaren, Karen and Maxi).
- We work on the poster information.

- **Wednesday 24th:**
- Wiki works.
- Trabajo con calcio competentes.

- **Friday 26th:**
- We work on the wiki information.
- We search for the coding sequence of ClbR and Cbh1.

- **Saturday 27th:**
- We take photos for our wiki.

- **Wednesday 31st:**
- Help other schools in lab.

- **September**

- **Saturday 3rd:**
- Wiki works.
- Design class for website (introduction).

- **Wednesday 7th:**
- Changes in the modeling design (final design).
- Wiki works.

- **Saturday 10th:**
- Production of news modeling parts.
- Wiki works.
- Design class to website.

- **Wednesday 14th:**
- Last website class.
- Wiki works.
- Reading of papers.

- **Friday 16th:**
- Lab work (Karen, Natalia, Maximiliano y Valentina).
- Wiki (Isabella)
- Reading of papers (Bastian y Maca)

- **Saturday 17th:**
- Wiki
- Little group rehearsal.

- **Wednesday 21st:**
- Wiki
- Progress of presentations.

- **Saturday 24th:**
- Wiki
- Plane our iGEM presentation.

- **Wednesday 28th:**
- Speak about our project.

- **Friday 30th:**
- Information and design of Wiki.

- **Octuber**

- **Saturday 1st:**
- Wiki Design.
- Poster Design.

- **Wednesday 5th:**
- Wiki
- Meeting with the Doctorate.

- **Friday 7th:**
- Preparation to the fair.

- **Saturday 8th:**
- Science fair in MIM (Chilean museum).

- **Monday 10th:**
- Wiki

- **Wednesday 12th:**
- Wiki
- Work with specialize in biotechnology.

- **Wednesday 14th:**
- Final design poster.
- Last details of experimental biological part.

- **Saturday 15th:**
- Final information and design to our wiki.
- Doctorate presentation.

- **Wednesday 19th:**
- iGEM Presentation Practice.

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