iGEM 2008 — Team Purdue

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# Team:Purdue/Notebook

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## Notebook Calendar

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| |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | May | | | | | | | | M | T | W | T | F | S | S | |  |  |  | [http://2008.igem.org/wiki/index.php?title=Purdue/1\_May\_2008&action=edit 1] | [http://2008.igem.org/wiki/index.php?title=Purdue/2\_May\_2008&action=edit 2] | [http://2008.igem.org/wiki/index.php?title=Purdue/3\_May\_2008&action=edit 3] | [http://2008.igem.org/wiki/index.php?title=Purdue/4\_May\_2008&action=edit 4] | | [http://2008.igem.org/wiki/index.php?title=Purdue/5\_May\_2008&action=edit 5] | [http://2008.igem.org/wiki/index.php?title=Purdue/6\_May\_2008&action=edit 6] | [http://2008.igem.org/wiki/index.php?title=Purdue/7\_May\_2008&action=edit 7] | [http://2008.igem.org/wiki/index.php?title=Purdue/8\_May\_2008&action=edit 8] | [http://2008.igem.org/wiki/index.php?title=Purdue/9\_May\_2008&action=edit 9] | [http://2008.igem.org/wiki/index.php?title=Purdue/10\_May\_2008&action=edit 10] | [http://2008.igem.org/wiki/index.php?title=Purdue/11\_May\_2008&action=edit 11] | | [http://2008.igem.org/wiki/index.php?title=Purdue/12\_May\_2008&action=edit 12] | [http://2008.igem.org/wiki/index.php?title=Purdue/13\_May\_2008&action=edit 13] | [http://2008.igem.org/wiki/index.php?title=Purdue/14\_May\_2008&action=edit 14] | [http://2008.igem.org/wiki/index.php?title=Purdue/15\_May\_2008&action=edit 15] | [http://2008.igem.org/wiki/index.php?title=Purdue/16\_May\_2008&action=edit 16] | [http://2008.igem.org/wiki/index.php?title=Purdue/17\_May\_2008&action=edit 17] | [http://2008.igem.org/wiki/index.php?title=Purdue/18\_May\_2008&action=edit 18] | | [http://2008.igem.org/wiki/index.php?title=Purdue/19\_May\_2008&action=edit 19] | [http://2008.igem.org/wiki/index.php?title=Purdue/20\_May\_2008&action=edit 20] | [http://2008.igem.org/wiki/index.php?title=Purdue/21\_May\_2008&action=edit 21] | [http://2008.igem.org/wiki/index.php?title=Purdue/22\_May\_2008&action=edit 22] | [http://2008.igem.org/wiki/index.php?title=Purdue/23\_May\_2008&action=edit 23] | [http://2008.igem.org/wiki/index.php?title=Purdue/24\_May\_2008&action=edit 24] | [http://2008.igem.org/wiki/index.php?title=Purdue/25\_May\_2008&action=edit 25] | | [http://2008.igem.org/wiki/index.php?title=Purdue/26\_May\_2008&action=edit 26] | [http://2008.igem.org/Purdue/27\_May\_2008 27] | [http://2008.igem.org/Purdue/28\_May\_2008 28] | [http://2008.igem.org/Purdue/29\_May\_2008 29] | [http://2008.igem.org/Purdue/30\_May\_2008 30] | [http://2008.igem.org/Purdue/31\_May\_2008 31] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | June | | | | | | | | M | T | W | T | F | S | S | |  |  |  |  |  |  | [http://2008.igem.org/Purdue/1\_June\_2008 1] | | [http://2008.igem.org/Purdue/2\_June\_2008 2] | [http://2008.igem.org/Purdue/3\_June\_2008 3] | [http://2008.igem.org/Purdue/4\_June\_2008 4] | [http://2008.igem.org/Purdue/5\_June\_2008 5] | [http://2008.igem.org/Purdue/6\_June\_2008 6] | [http://2008.igem.org/Purdue/7\_June\_2008 7] | [http://2008.igem.org/wiki/index.php?title=Purdue/8\_June\_2008&action=edit 8] | | [http://2008.igem.org/Purdue/9\_June\_2008 9] | [http://2008.igem.org/Purdue/10\_June\_2008 10] | [http://2008.igem.org/Purdue/11\_June\_2008 11] | [http://2008.igem.org/Purdue/12\_June\_2008 12] | [http://2008.igem.org/Purdue/13\_June\_2008 13] | [http://2008.igem.org/Purdue/14\_June\_2008 14] | [http://2008.igem.org/Purdue/15\_June\_2008 15] | | [http://2008.igem.org/Purdue/16\_June\_2008 16] | [http://2008.igem.org/Purdue/17\_June\_2008 17] | [http://2008.igem.org/Purdue/18\_June\_2008 18] | [http://2008.igem.org/Purdue/19\_June\_2008 19] | [http://2008.igem.org/Purdue/20\_June\_2008 20] | [http://2008.igem.org/Purdue/21\_June\_2008 21] | [http://2008.igem.org/Purdue/22\_June\_2008 22] | | [http://2008.igem.org/Purdue/23\_June\_2008 23] | [http://2008.igem.org/Purdue/24\_June\_2008 24] | [http://2008.igem.org/Purdue/25\_June\_2008 25] | [http://2008.igem.org/Purdue/26\_June\_2008 26] | [http://2008.igem.org/Purdue/27\_June\_2008 27] | [http://2008.igem.org/Purdue/28\_June\_2008 28] | [http://2008.igem.org/Purdue/29\_June\_2008 29] | | [http://2008.igem.org/Purdue/30\_June\_2008 30] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | July | | | | | | | | M | T | W | T | F | S | S | |  | [http://2008.igem.org/Purdue/1\_July\_2008 1] | [http://2008.igem.org/Purdue/2\_July\_2008 2] | [http://2008.igem.org/Purdue/3\_July\_2008 3] | [http://2008.igem.org/Purdue/4\_July\_2008 4] | [http://2008.igem.org/Purdue/5\_July\_2008 5] | [http://2008.igem.org/Purdue/6\_July\_2008 6] | | [http://2008.igem.org/Purdue/7\_July\_2008 7] | [http://2008.igem.org/Purdue/8\_July\_2008 8] | [http://2008.igem.org/Purdue/9\_July\_2008 9] | [http://2008.igem.org/Purdue/10\_July\_2008 10] | [http://2008.igem.org/Purdue/11\_July\_2008 11] | [http://2008.igem.org/Purdue/12\_July\_2008 12] | [http://2008.igem.org/Purdue/13\_July\_2008 13] | | [http://2008.igem.org/Purdue/14\_July\_2008 14] | [http://2008.igem.org/Purdue/15\_July\_2008 15] | [http://2008.igem.org/Purdue/16\_July\_2008 16] | [http://2008.igem.org/Purdue/17\_July\_2008 17] | [http://2008.igem.org/Purdue/18\_July\_2008 18] | [http://2008.igem.org/Purdue/19\_July\_2008 19] | [http://2008.igem.org/Purdue/20\_July\_2008 20] | | [http://2008.igem.org/Purdue/21\_July\_2008 21] | [http://2008.igem.org/Purdue/22\_July\_2008 22] | [http://2008.igem.org/Purdue/23\_July\_2008 23] | [http://2008.igem.org/Purdue/24\_July\_2008 24] | [http://2008.igem.org/Purdue/25\_July\_2008 25] | [http://2008.igem.org/Purdue/26\_July\_2008 26] | [http://2008.igem.org/Purdue/27\_July\_2008 27] | | [http://2008.igem.org/Purdue/28\_July\_2008 28] | [http://2008.igem.org/Purdue/29\_July\_2008 29] | [http://2008.igem.org/Purdue/30\_July\_2008 30] | [http://2008.igem.org/Purdue/31\_July\_2008 31] | | [![Purdue-logo.jpg](/wiki/images/3/30/Purdue-logo.jpg)](/File%3APurdue-logo.jpg) |
| |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | August | | | | | | | | M | T | W | T | F | S | S | |  |  |  |  | [http://2008.igem.org/Purdue/1\_August\_2008 1] | [http://2008.igem.org/Purdue/2\_August\_2008 2] | [http://2008.igem.org/Purdue/3\_August\_2008 3] | | [http://2008.igem.org/Purdue/4\_August\_2008 4] | [http://2008.igem.org/Purdue/5\_August\_2008 5] | [http://2008.igem.org/Purdue/6\_August\_2008 6] | [http://2008.igem.org/Purdue/7\_August\_2008 7] | [http://2008.igem.org/Purdue/8\_August\_2008 8] | [http://2008.igem.org/Purdue/9\_August\_2008 9] | [http://2008.igem.org/Purdue/10\_August\_2008 10] | | [http://2008.igem.org/Purdue/11\_August\_2008 11] | [http://2008.igem.org/Purdue/12\_August\_2008 12] | [http://2008.igem.org/Purdue/13\_August\_2008 13] | [http://2008.igem.org/Purdue/14\_August\_2008 14] | [http://2008.igem.org/Purdue/15\_August\_2008 15] | [http://2008.igem.org/Purdue/16\_August\_2008 16] | [http://2008.igem.org/Purdue/17\_August\_2008 17] | | [http://2008.igem.org/Purdue/18\_August\_2008 18] | [http://2008.igem.org/Purdue/19\_August\_2008 19] | [http://2008.igem.org/Purdue/20\_August\_2008 20] | [http://2008.igem.org/Purdue/21\_August\_2008 21] | [http://2008.igem.org/Purdue/22\_August\_2008 22] | [http://2008.igem.org/Purdue/23\_August\_2008 23] | [http://2008.igem.org/Purdue/24\_August\_2008 24] | | [http://2008.igem.org/Purdue/25\_August\_2008 25] | [http://2008.igem.org/Purdue/26\_August\_2008 26] | [http://2008.igem.org/Purdue/27\_August\_2008 27] | [http://2008.igem.org/Purdue/28\_August\_2008 28] | [http://2008.igem.org/Purdue/29\_August\_2008 29] | [http://2008.igem.org/Purdue/30\_August\_2008 30] | [http://2008.igem.org/Purdue/31\_August\_2008 31] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | September | | | | | | | | M | T | W | T | F | S | S | | [http://2008.igem.org/Purdue/1\_September\_2008 1] | [http://2008.igem.org/Purdue/2\_September\_2008 2] | [http://2008.igem.org/Purdue/3\_September\_2008 3] | [http://2008.igem.org/Purdue/4\_September\_2008 4] | [http://2008.igem.org/Purdue/5\_September\_2008 5] | [http://2008.igem.org/Purdue/6\_September\_2008 6] | [http://2008.igem.org/Purdue/7\_September\_2008 7] | | [http://2008.igem.org/Purdue/8\_September\_2008 8] | [http://2008.igem.org/Purdue/9\_September\_2008 9] | [http://2008.igem.org/Purdue/10\_September\_2008 10] | [http://2008.igem.org/Purdue/11\_September\_2008 11] | [http://2008.igem.org/Purdue/12\_September\_2008 12] | [http://2008.igem.org/Purdue/13\_September\_2008 13] | [http://2008.igem.org/Purdue/14\_September\_2008 14] | | [http://2008.igem.org/Purdue/15\_September\_2008 15] | [http://2008.igem.org/Purdue/16\_September\_2008 16] | [http://2008.igem.org/Purdue/17\_September\_2008 17] | [http://2008.igem.org/Purdue/18\_September\_2008 18] | [http://2008.igem.org/Purdue/19\_September\_2008 19] | [http://2008.igem.org/Purdue/20\_September\_2008 20] | [http://2008.igem.org/Purdue/21\_September\_2008 21] | | [http://2008.igem.org/Purdue/22\_September\_2008 22] | [http://2008.igem.org/Purdue/23\_September\_2008 23] | [http://2008.igem.org/Purdue/24\_September\_2008 24] | [http://2008.igem.org/Purdue/25\_September\_2008 25] | [http://2008.igem.org/Purdue/26\_September\_2008 26] | [http://2008.igem.org/Purdue/27\_September\_2008 27] | [http://2008.igem.org/Purdue/28\_September\_2008 28] | | [http://2008.igem.org/Purdue/29\_September\_2008 29] | [http://2008.igem.org/Purdue/30\_September\_2008 30] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | October | | | | | | | | M | T | W | T | F | S | S | |  |  | [http://2008.igem.org/Purdue/1\_October\_2008 1] | [http://2008.igem.org/Purdue/2\_October\_2008 2] | [http://2008.igem.org/Purdue/3\_October\_2008 3] | [http://2008.igem.org/Purdue/4\_October\_2008 4] | [http://2008.igem.org/Purdue/5\_October\_2008 5] | | [http://2008.igem.org/Purdue/6\_October\_2008 6] | [http://2008.igem.org/Purdue/7\_October\_2008 7] | [http://2008.igem.org/Purdue/8\_October\_2008 8] | [http://2008.igem.org/Purdue/9\_October\_2008 9] | [http://2008.igem.org/Purdue/10\_October\_2008 10] | [http://2008.igem.org/Purdue/11\_October\_2008 11] | [http://2008.igem.org/Purdue/12\_October\_2008 12] | | [http://2008.igem.org/Purdue/13\_October\_2008 13] | [http://2008.igem.org/Purdue/14\_October\_2008 14] | [http://2008.igem.org/Purdue/15\_October\_2008 15] | [http://2008.igem.org/Purdue/16\_October\_2008 16] | [http://2008.igem.org/Purdue/17\_October\_2008 17] | [http://2008.igem.org/Purdue/18\_October\_2008 18] | [http://2008.igem.org/Purdue/19\_October\_2008 19] | | [http://2008.igem.org/Purdue/20\_October\_2008 20] | [http://2008.igem.org/Purdue/21\_October\_2008 21] | [http://2008.igem.org/Purdue/22\_October\_2008 22] | [http://2008.igem.org/Purdue/23\_October\_2008 23] | [http://2008.igem.org/Purdue/24\_October\_2008 24] | [http://2008.igem.org/Purdue/25\_October\_2008 25] | [http://2008.igem.org/Purdue/26\_October\_2008 26] | | [http://2008.igem.org/Purdue/27\_October\_2008 27] | [http://2008.igem.org/Purdue/28\_October\_2008 28] | [http://2008.igem.org/Purdue/29\_October\_2008 29] | [http://2008.igem.org/Purdue/30\_October\_2008 30] | [http://2008.igem.org/Purdue/31\_October\_2008 31] | |

## Summary of Key Notebook Pages

**June 2, 4** Craig's Milk pH Data

**June 10** Second run and results of Milk pH data.

**June 11** Group meeting and summary of UV idea

**June 13** List of parts to use

**June 23** First attempt at transformation

**June 25** Second attempt at transformation

**July 7** Change in plan, new list of parts

**August 12** UV flux test

**September 2** Digestion protocol

**September 17** Miniprep of SOS (redo this part because ran off the gel the first time)

**September 18** Digestion of SOS

**September 23** Gel purification

**September 25 and October 2** Ligation procedure and ligation: take 2

**October 9, 10** Preliminary testing of transformed bacteria

**October 16** Ligation III, Transformation, and results of testing...

**October 26** Final Miniprep and Digestion (+ picture of a gel!)

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