iGEM 2013 — Team SUSTC-Shenzhen-A

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# Team:SUSTC-Shenzhen-A/Notebook

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# Game theory

Stategy for the Classic Prisoners' Dilemma.

* [Notebook](#notebook)
* [Protocol](#protocol)
* [Calendar](#calendar)

# Notebook

We started our project in middle July. You can find every detail by clicking the date.

18th July to 10th Aug: Training

12th Aug to 17th Sep: Project and biobricks.

# Protocol

[Preparation of LB culture](/index.Preparation%20of%20LB%20culture)
[Preparation of some antibiotic solution](/index.Preparation%20of%20some%20antibiotic%20solution)
[Transformation](/index.Transformation)
[Preservation of the bacteria](/index.Preservation%20of%20the%20bacteria)
[Extract the target DNA](/index.Extract%20the%20target%20DNA)
[The use of spectrometer](/index.The%20use%20of%20spectrometer)
[Usage of PCR-Cycler](/index.Usage%20of%20PCR-Cycler)
[Usage of ultraviolet spectrophotometer](/index.Usage%20of%20ultraviolet%20spectrophotometer)
 [Preparation for the M9 culture](/index.%20Preparation%20for%20the%20M9%20culture)
[Preparation of the nitrate LB culture](/index.Preparation%20of%20the%20nitrate%20LB%20culture)
 [Usage of FCM](/index.Usage%20of%20FCM)
[Preparation of the gel](/index.Preparation%20of%20the%20gel)
 [Recycle of the DNA in the gel and deal with gel](/index.Recycle%20of%20the%20DNA%20in%20the%20gel%20and%20deal%20with%20gel)
[The recycle of the products of the PCR](/index.The%20recycle%20of%20the%20products%20of%20the%20PCR)
[The use of the microplate reader](/index.The%20use%20of%20the%20microplate%20reader)
[Check he function of the xylose and the nitrate inducible promote](/index.%20check%20the%20function%20of%20the%20xylose%20and%20the%20nitrate%20inducible%20promote)

# Calendar

July

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| M | T | W | T | F | S | S |
| [1](https://2013.igem.org/wiki/index.php?title=SUSTC/1_July_2013&action=edit) | [2](https://2013.igem.org/wiki/index.php?title=SUSTC/2_July_2013&action=edit) | [3](https://2013.igem.org/wiki/index.php?title=SUSTC/3_July_2013&action=edit) | [4](https://2013.igem.org/wiki/index.php?title=SUSTC/4_July_2013&action=edit) | [5](https://2013.igem.org/wiki/index.php?title=SUSTC/5_July_2013&action=edit) | [6](https://2013.igem.org/wiki/index.php?title=SUSTC/6_July_2013&action=edit) | [7](https://2013.igem.org/wiki/index.php?title=SUSTC/7_July_2013&action=edit) |
| [8](https://2013.igem.org/wiki/index.php?title=SUSTC/8_July_2013&action=edit) | [9](https://2013.igem.org/wiki/index.php?title=SUSTC/9_July_2013&action=edit) | [10](https://2013.igem.org/wiki/index.php?title=SUSTC/10_July_2013&action=edit) | [11](https://2013.igem.org/wiki/index.php?title=SUSTC/11_July_2013&action=edit) | [12](https://2013.igem.org/wiki/index.php?title=SUSTC/12_July_2013&action=edit) | [13](https://2013.igem.org/wiki/index.php?title=SUSTC/13_July_2013&action=edit) | [14](https://2013.igem.org/wiki/index.php?title=SUSTC/14_July_2013&action=edit) |
| [15](https://2013.igem.org/wiki/index.php?title=SUSTC/15_July_2013&action=edit) | [16](https://2013.igem.org/wiki/index.php?title=SUSTC/16_July_2013&action=edit) | [17](https://2013.igem.org/wiki/index.php?title=SUSTC/17_July_2013&action=edit) | [18](https://2013.igem.org/SUSTC/18_July_2013) | [19](https://2013.igem.org/SUSTC/19_July_2013) | [20](https://2013.igem.org/wiki/index.php?title=SUSTC/20_July_2013&action=edit) | [21](https://2013.igem.org/wiki/index.php?title=SUSTC/21_July_2013&action=edit) |
| [22](https://2013.igem.org/SUSTC/22_July_2013) | [23](https://2013.igem.org/wiki/index.php?title=SUSTC/23_July_2013&action=edit) | [24](https://2013.igem.org/wiki/index.php?title=SUSTC/24_July_2013&action=edit) | [25](https://2013.igem.org/SUSTC/25_July_2013) | [26](https://2013.igem.org/wiki/index.php?title=SUSTC/26_July_2013&action=edit) | [27](https://2013.igem.org/wiki/index.php?title=SUSTC/27_July_2013&action=edit) | [28](https://2013.igem.org/SUSTC/28_July_2013) |
| [29](https://2013.igem.org/wiki/index.php?title=SUSTC/29_July_2013&action=edit) | [30](https://2013.igem.org/wiki/index.php?title=SUSTC/30_July_2013&action=edit) | [31](https://2013.igem.org/wiki/index.php?title=SUSTC/31_July_2013&action=edit) |

August

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| M | T | W | T | F | S | S |
|  |  |  | [1](https://2013.igem.org/wiki/index.php?title=SUSTC/1_August_2013&action=edit) | [2](https://2013.igem.org/wiki/index.php?title=SUSTC/2_August_2013&action=edit) | [3](https://2013.igem.org/wiki/index.php?title=SUSTC/3_August_2013&action=edit) | [4](https://2013.igem.org/wiki/index.php?title=SUSTC/4_August_2013&action=edit) |
| [5](https://2013.igem.org/wiki/index.php?title=SUSTC/5_August_2013&action=edit) | [6](https://2013.igem.org/wiki/index.php?title=SUSTC/6_August_2013&action=edit) | [7](https://2013.igem.org/wiki/index.php?title=SUSTC/7_August_2013&action=edit) | [8](https://2013.igem.org/wiki/index.php?title=SUSTC/8_August_2013&action=edit) | [9](https://2013.igem.org/SUSTC/9_August_2013) | [10](https://2013.igem.org/SUSTC/10_August_2013) | [11](https://2013.igem.org/wiki/index.php?title=SUSTC/11_August_2013&action=edit) |
| [12](https://2013.igem.org/SUSTC/12_August_2013) | [13](https://2013.igem.org/SUSTC/13_August_2013) | [14](https://2013.igem.org/SUSTC/14_August_2013) | [15](https://2013.igem.org/SUSTC/15_August_2013) | [16](https://2013.igem.org/SUSTC/16_August_2013) | [17](https://2013.igem.org/SUSTC/17_August_2013) | [18](https://2013.igem.org/SUSTC/18_August_2013) |
| [19](https://2013.igem.org/SUSTC/19_August_2013) | [20](https://2013.igem.org/SUSTC/20_August_2013) | [21](https://2013.igem.org/SUSTC/21_August_2013) | [22](https://2013.igem.org/SUSTC/22_August_2013) | [23](https://2013.igem.org/wiki/index.php?title=SUSTC/23_August_2013&action=edit) | [24](https://2013.igem.org/SUSTC/24_August_2013) | [25](https://2013.igem.org/SUSTC/25_August_2013) |
| [26](https://2013.igem.org/SUSTC/26_August_2013) | [27](https://2013.igem.org/SUSTC/27_August_2013) | [28](https://2013.igem.org/SUSTC/28_August_2013) | [29](https://2013.igem.org/SUSTC/29_August_2013) | [30](https://2013.igem.org/SUSTC/30_August_2013) | [31](https://2013.igem.org/SUSTC/31_August_2013) |

September

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| M | T | W | T | F | S | S |
|  |  |  |  |  |  | [1](https://2013.igem.org/SUSTC/1_September_2013) |
| [2](https://2013.igem.org/wiki/index.php?title=SUSTC/2_September_2013&action=edit) | [3](https://2013.igem.org/SUSTC/3_September_2013) | [4](https://2013.igem.org/SUSTC/4_September_2013) | [5](https://2013.igem.org/SUSTC/5_September_2013) | [6](https://2013.igem.org/wiki/index.php?title=SUSTC/6_September_2013&action=edit) | [7](https://2013.igem.org/SUSTC/7_September_2013) | [8](https://2013.igem.org/SUSTC/8_September_2013) |
| [9](https://2013.igem.org/SUSTC/9_September_2013) | [10](https://2013.igem.org/SUSTC/10_September_2013) | [11](https://2013.igem.org/SUSTC/11_September_2013) | [12](https://2013.igem.org/SUSTC/12_September_2013) | [13](https://2013.igem.org/SUSTC/13_September_2013) | [14](https://2013.igem.org/SUSTC/14_September_2013) | [15](https://2013.igem.org/SUSTC/15_September_2013) |
| [16](https://2013.igem.org/SUSTC/16_September_2013) | [17](https://2013.igem.org/SUSTC/17_September_2013) | [18](https://2013.igem.org/wiki/index.php?title=SUSTC/18_September_2013&action=edit) | [19](https://2013.igem.org/wiki/index.php?title=SUSTC/19_September_2013&action=edit) | [20](https://2013.igem.org/wiki/index.php?title=SUSTC/20_September_2013&action=edit) | [21](https://2013.igem.org/wiki/index.php?title=SUSTC/21_September_2013&action=edit) | [22](https://2013.igem.org/wiki/index.php?title=SUSTC/22_September_2013&action=edit) |
| [23](https://2013.igem.org/wiki/index.php?title=SUSTC/23_September_2013&action=edit) | [24](https://2013.igem.org/wiki/index.php?title=SUSTC/24_September_2013&action=edit) | [25](https://2013.igem.org/wiki/index.php?title=SUSTC/25_September_2013&action=edit) | [26](https://2013.igem.org/wiki/index.php?title=SUSTC/26_September_2013&action=edit) | [27](https://2013.igem.org/wiki/index.php?title=SUSTC/27_September_2013&action=edit) | [28](https://2013.igem.org/wiki/index.php?title=SUSTC/28_September_2013&action=edit) | [29](https://2013.igem.org/wiki/index.php?title=SUSTC/29_September_2013&action=edit) |
| [30](https://2013.igem.org/wiki/index.php?title=SUSTC/30_September_2013&action=edit) |

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