iGEM 2018 — Team IISER-Kolkata

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# Team:IISER-Kolkata/Notebook

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[Home](https://2018.igem.org/Team%3AIISER-Kolkata)

[Project](https://2018.igem.org/Team%3AIISER-Kolkata/Problem)

[Lab](https://2018.igem.org/Team%3AIISER-Kolkata/Deletion)

[Achievements](https://2018.igem.org/Team%3AIISER-Kolkata/Results)

[Humanity](https://2018.igem.org/Team%3AIISER-Kolkata/Human_Practices)

[Team](https://2018.igem.org/Team%3AIISER-Kolkata/Team)

[Deletion](https://2018.igem.org/Team%3AIISER-Kolkata/Deletion)

[Assembly](https://2018.igem.org/Team%3AIISER-Kolkata/Assembly)

[Parts](https://2018.igem.org/Team%3AIISER-Kolkata/Parts)

[Notebook](https://2018.igem.org/Team%3AIISER-Kolkata/Notebook)

[Protocols](https://2018.igem.org/Team%3AIISER-Kolkata/Protocols)

[Safety](https://2018.igem.org/Team%3AIISER-Kolkata/Safety)

[InterLab](https://2018.igem.org/Team%3AIISER-Kolkata/InterLab)

# Notebook

Team IISER Kolkata maintained a very elaborate lab record keenly keeping account of its progress through out the journey. Due to technical issues, the lab record is presented to you in the form a downloadable PDF file.
Please [click here](https://static.igem.org/mediawiki/2018/4/47/T--IISER-Kolkata--Notebook.pdf) to view and download the NOTEBOOK.


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# Team:IISER-Kolkata/Experiments

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[HOME](https://2018.igem.org/Team%3AIISER-Kolkata)

TEAM

[Team Members](https://2018.igem.org/Team%3AIISER-Kolkata/Team)
[Collaborations](https://2018.igem.org/Team%3AIISER-Kolkata/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AIISER-Kolkata/Description)
[Design](https://2018.igem.org/Team%3AIISER-Kolkata/Design)
[Experiments](https://2018.igem.org/Team%3AIISER-Kolkata/Experiments)
[Notebook](https://2018.igem.org/Team%3AIISER-Kolkata/Notebook)
[InterLab](https://2018.igem.org/Team%3AIISER-Kolkata/InterLab)
[Model](https://2018.igem.org/Team%3AIISER-Kolkata/Model)
[Results](https://2018.igem.org/Team%3AIISER-Kolkata/Results)
[Demonstrate](https://2018.igem.org/Team%3AIISER-Kolkata/Demonstrate)
[Improve](https://2018.igem.org/Team%3AIISER-Kolkata/Improve)
[Attributions](https://2018.igem.org/Team%3AIISER-Kolkata/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AIISER-Kolkata/Parts)
[Basic Parts](https://2018.igem.org/Team%3AIISER-Kolkata/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AIISER-Kolkata/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AIISER-Kolkata/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AIISER-Kolkata/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AIISER-Kolkata/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AIISER-Kolkata/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AIISER-Kolkata/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AIISER-Kolkata/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AIISER-Kolkata/Hardware)
[Measurement](https://2018.igem.org/Team%3AIISER-Kolkata/Measurement)
[Model](https://2018.igem.org/Team%3AIISER-Kolkata/Model)
[Plant](https://2018.igem.org/Team%3AIISER-Kolkata/Plant)
[Software](https://2018.igem.org/Team%3AIISER-Kolkata/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=IISER-Kolkata)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:IISER-Kolkata/Results

[![](https://static.igem.org/mediawiki/2018/c/cd/T--IISER-Kolkata--bacman.jpg)](https://2018.igem.org/Team%3AIISER-Kolkata)

[Home](https://2018.igem.org/Team%3AIISER-Kolkata)

[Project](https://2018.igem.org/Team%3AIISER-Kolkata/Problem)

[Lab](https://2018.igem.org/Team%3AIISER-Kolkata/Deletion)

[Achievements](https://2018.igem.org/Team%3AIISER-Kolkata/Results)

[Humanity](https://2018.igem.org/Team%3AIISER-Kolkata/Human_Practices)

[Team](https://2018.igem.org/Team%3AIISER-Kolkata/Team)

[Results](https://2018.igem.org/Team%3AIISER-Kolkata/Results)

[Medal Criteria](https://2018.igem.org/Team%3AIISER-Kolkata/Medals)

[Jamboree](https://2018.igem.org/Team%3AIISER-Kolkata/Jamboree)

# Results

Team IISER Kolkata proudly brags about the following achievements it has accomplished:

* Our project proposal was selected among the top 5 in the nation by the Department of Biotechnology (DBT), Govt. of India to receive a grant of Rs. 1 million to participate in iGEM 2018 and represent the institute and our country at this prestigious international platform.
  ![](https://static.igem.org/mediawiki/2018/6/6c/T--IISER-Kolkata--iBEC.jpg)
[DBT Announcement of pre-iGEM India Level Competition Results](http://www.dbtindia.nic.in/announcement-of-ibec-pre-igem-result/).* Student members of the team successfully cloned a gene from the wild by constructing cDNA from the RNA pool of S. pombe and then PCR amplifying the desired gene.
* Student members of the team successfully completed the Interlab exercise and became part of the international collaborative effort to standardize GFP expression and measurement assays by positively contributing their data.
* Student members of the team successfully obtained a clone which has now been submitted to the registry as part Bba\_K2773000.
* Student members of the team achieved the Best Poster Award at the Modern Trends in Microbiology Symposium at St. Xavier’s College, Kolkata on 11th October, 2018.
  ![](https://static.igem.org/mediawiki/2018/b/bb/T--IISER-Kolkata--MTIM.jpg)