iGEM 2020 — Team FAFU-CHINA

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:FAFU-CHINA/Notebook

Team:FAFU-CHINA - 2020.igem.org

![logo](https://static.igem.org/mediawiki/2020/c/c1/T--FAFU-CHINA--logo.png)
[FAFU-CHINA](https://2020.igem.org/Team%3AFAFU-CHINA)

* PROJECT
  + [Background](https://2020.igem.org/Team%3AFAFU-CHINA/Background)
  + [Description](https://2020.igem.org/Team%3AFAFU-CHINA/Description)
  + [Demonstrate](https://2020.igem.org/Team%3AFAFU-CHINA/Demonstration)
  + [Measure](https://2020.igem.org/Team%3AFAFU-CHINA/Measure)
  + [Notebook](https://2020.igem.org/Team%3AFAFU-CHINA/Notebook)
  + [Protocol](https://2020.igem.org/Team%3AFAFU-CHINA/Protocol)
  + [Engineering](https://2020.igem.org/Team%3AFAFU-CHINA/Engineering)
  + [Implementation](https://2020.igem.org/Team%3AFAFU-CHINA/Implementation)
  + [Poster](https://2020.igem.org/Team%3AFAFU-CHINA/Poster)
* MODEL
  + [Model](https://2020.igem.org/Team%3AFAFU-CHINA/Model)
  + [Protein Modeling](https://2020.igem.org/Team%3AFAFU-CHINA/Protein_Modeling)
* HUMAN PRACTICE
  + [HP](https://2020.igem.org/Team%3AFAFU-CHINA/Human_Practices)
  + [Contribution](https://2020.igem.org/Team%3AFAFU-CHINA/Contribution)
  + [Collaborations](https://2020.igem.org/Team%3AFAFU-CHINA/Collaborations)
  + [Partnership](https://2020.igem.org/Team%3AFAFU-CHINA/Partnership)
  + [Science Communications](https://2020.igem.org/Team%3AFAFU-CHINA/Education)
  + [Excellence](https://2020.igem.org/Team%3AFAFU-CHINA/Excellence_in_Another_Area)
* TEAM
  + [Members](https://2020.igem.org/Team%3AFAFU-CHINA/Members)
  + [Attributions](https://2020.igem.org/Team%3AFAFU-CHINA/Attribution)
* PARTS
  + [Basic Parts](https://2020.igem.org/Team%3AFAFU-CHINA/Basic_Parts)
  + [Composite Parts](https://2020.igem.org/Team%3AFAFU-CHINA/Composite_Parts)
* AWARDS
  + [Bronze](https://2020.igem.org/Team%3AFAFU-CHINA/Bronze)
  + [Sliver](https://2020.igem.org/Team%3AFAFU-CHINA/Silver)
  + [Gold](https://2020.igem.org/Team%3AFAFU-CHINA/Gold)

[![](https://static.igem.org/mediawiki/2020/f/f3/T--FAFU-CHINA--Top.png)](#top)

# Notebook

# December 2019

Senior students promoted iGEM and recruited candidates who are interested in joining the iGEM team.

# January 2020

iGEM conducted several brainstorms on topic selection online

# March 2020

We have identified the final personnel involved in igem and formulated several research directions

# May 2020

After many discussions, we determined our research topic

We started cooperation with Beijing Normal University on project planning

# July 2020

We participated in the East China exchange meeting. Among them, Mr. Zhu Tianze from Beijing Zino Technology gave us suggestions, and we also participated in the drift bottle project.

Participated in the online exchange meeting in East China on the 18th of this month. In the meeting we explained our experimental design and project prospects in detail.
We communicated with Northeast Forestry University on gene circuit design and project planning, social practice, etc.

# August 2020

From 3th to 10st of this month, Together with THU, PKU, CAU, and BNU, we launched a one-week open educational popularization of synthetic biology online.

On 26th, we discussed the design rationality of the integrated fermentation system with BNU online.

From 28th to 31st of this month, we participated in the CCIC exchange meeting

# September 5 to September 13, 2020

After the epidemic went back to school, I came to the school and asked the instructor. I was guided by PI in the experimental design and provided us with an experimental site.

We interviewed Academician Lin Zhanxi and discussed the problems and prospects of Juncao fermentation to produce fiber bioethanol

We held popular science activities on synthetic biology in the school, and the safety issues brought by synthetic biology

# September 14 to September 18, 2020

We officially started our experiment, we began to screen cellulolytic bacteria from the soil and successfully isolated a single colony

We studied and designed our primers and purchased the reagents and kits needed for future experiments

On 16th, we discussed the Problems encountered during the experiment with BNU online.

# September 18 to September 22, 2020

We successfully constructed the B3 non-gate fragment into the Pet28b plasmid.

# September 22 to October 1, 2020

We successfully transformed the recombinant plasmid into BL21 competent cells and got the correct transformants.

We continue to purify the isolated single colony and save it for backup.

# October 2 to October 9, 2020

We interviewed teacher Liu Bin, and he suggested that we use Beauveria bassiana against our degrading enzyme system and pay attention to the best harvest period for Juncao suitable for fermentation.

We compare the decomposed cellulolytic bacteria and white pulp bacteria in various aspects, and use the DNS method to test and verify the enzyme activity of our cellulolytic bacteria.

We measured the growth curve of BL21.

# October 10 to October 19, 2020

We continue to purify the separated cellulolytic single colony,

We used the hemicellulose kit purchased by Solarbio to determine the content of hemicellulose in the grass powder.

We successfully constructed RFP and GFP into the PET28 plasmid, and transformed into DH5α, and saw green fluorescence.

We interviewed Jingfeng Technology Co., Ltd. and Xinda Knitting Co., Ltd. We visited their textile factories, and the staff discussed with us the economic problems of using Juncao fermentation to produce cellulose.

# October 20 to October 27

We communicated online with Jilin University and learned from each other’s work system

* December 2019
* January 2020
* March 2020
* May 2020
* July 2020
* August 2020
* September 2020
* October 2020

![](https://static.igem.org/mediawiki/2020/4/44/T--FAFU-CHINA--footer.png)

©Fujian Agriculture and Forestry University

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:FAFU-CHINA/Engineering

Team:FAFU-CHINA - 2020.igem.org

![logo](https://static.igem.org/mediawiki/2020/c/c1/T--FAFU-CHINA--logo.png)
[FAFU-CHINA](https://2020.igem.org/Team%3AFAFU-CHINA)

* PROJECT
  + [Background](https://2020.igem.org/Team%3AFAFU-CHINA/Background)
  + [Description](https://2020.igem.org/Team%3AFAFU-CHINA/Description)
  + [Demonstrate](https://2020.igem.org/Team%3AFAFU-CHINA/Demonstration)
  + [Measure](https://2020.igem.org/Team%3AFAFU-CHINA/Measure)
  + [Notebook](https://2020.igem.org/Team%3AFAFU-CHINA/Notebook)
  + [Protocol](https://2020.igem.org/Team%3AFAFU-CHINA/Protocol)
  + [Engineering](https://2020.igem.org/Team%3AFAFU-CHINA/Engineering)
  + [Implementation](https://2020.igem.org/Team%3AFAFU-CHINA/Implementation)
  + [Poster](https://2020.igem.org/Team%3AFAFU-CHINA/Poster)
* MODEL
  + [Model](https://2020.igem.org/Team%3AFAFU-CHINA/Model)
  + [Protein Modeling](https://2020.igem.org/Team%3AFAFU-CHINA/Protein_Modeling)
* HUMAN PRACTICE
  + [HP](https://2020.igem.org/Team%3AFAFU-CHINA/Human_Practices)
  + [Contribution](https://2020.igem.org/Team%3AFAFU-CHINA/Contribution)
  + [Collaborations](https://2020.igem.org/Team%3AFAFU-CHINA/Collaborations)
  + [Partnership](https://2020.igem.org/Team%3AFAFU-CHINA/Partnership)
  + [Science Communications](https://2020.igem.org/Team%3AFAFU-CHINA/Education)
  + [Excellence](https://2020.igem.org/Team%3AFAFU-CHINA/Excellence_in_Another_Area)
* TEAM
  + [Members](https://2020.igem.org/Team%3AFAFU-CHINA/Members)
  + [Attributions](https://2020.igem.org/Team%3AFAFU-CHINA/Attribution)
* PARTS
  + [Basic Parts](https://2020.igem.org/Team%3AFAFU-CHINA/Basic_Parts)
  + [Composite Parts](https://2020.igem.org/Team%3AFAFU-CHINA/Composite_Parts)
* AWARDS
  + [Bronze](https://2020.igem.org/Team%3AFAFU-CHINA/Bronze)
  + [Sliver](https://2020.igem.org/Team%3AFAFU-CHINA/Silver)
  + [Gold](https://2020.igem.org/Team%3AFAFU-CHINA/Gold)

# Engineering

[![](https://static.igem.org/mediawiki/2020/f/f3/T--FAFU-CHINA--Top.png)](#top)

![](https://static.igem.org/mediawiki/2020/4/44/T--FAFU-CHINA--footer.png)

©Fujian Agriculture and Forestry University

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:FAFU-CHINA/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:FAFU-CHINA/Results

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)