iGEM 2017 — Team Oxford

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Oxford/Notebook

[![](https://static.igem.org/mediawiki/2017/8/82/T--oxford--see--cruzi--logo--inverted.png)](https://2017.igem.org/Team%3AOxford)

* [HOME](https://2017.igem.org/Team%3AOxford)
* PROJECT
  + [Description](https://2017.igem.org/Team%3AOxford/Description)
  + [Chagas Disease](https://2017.igem.org/Team%3AOxford/Chagas_Disease)
  + [Chagas Public Policy](https://2017.igem.org/Team%3AOxford/Chagas_Public_Policy)
  + [Design](https://2017.igem.org/Team%3AOxford/Design)
  + [Parts](https://2017.igem.org/Team%3AOxford/Parts)
  + [Applied Design](https://2017.igem.org/Team%3AOxford/Applied_Design)
  + [--Developing our Design](https://2017.igem.org/Team%3AOxford/Applied_Design_Developing)
  + [--Our Solution](https://2017.igem.org/Team%3AOxford/Applied_Design_Solution)
  + [--Implementation](https://2017.igem.org/Team%3AOxford/Applied_Design_Implementation)
  + [Safety](https://2017.igem.org/Team%3AOxford/Safety)
  + [Cell Free Report](https://2017.igem.org/Team%3AOxford/Cell_Free_Report)
  + [Initial Ideas](https://2017.igem.org/Team%3AOxford/InitialIdeas)
* WET LAB
  + [Overview](https://2017.igem.org/Team%3AOxford/Overview_Wet_Lab)
  + [Results (Cloning)](https://2017.igem.org/Team%3AOxford/Cloning)
  + [Results (DNA-Based system)](https://2017.igem.org/Team%3AOxford/Results_DNA)
  + [Results (Protein-Based system)](https://2017.igem.org/Team%3AOxford/Results_Protein)
  + [Future Experiments](https://2017.igem.org/Team%3AOxford/Future_experiments)
  + [Protocols](https://2017.igem.org/Team%3AOxford/Protocols)
  + [Notebook](https://2017.igem.org/Team%3AOxford/Notebook)
  + [Interlab](https://2017.igem.org/Team%3AOxford/InterLab)
  + [Measurement](https://2017.igem.org/Team%3AOxford/Measurement)
* DRY LAB
  + [Overview](https://2017.igem.org/Team%3AOxford/Model)
  + [DNA-Based Model](https://2017.igem.org/Team%3AOxford/DNA_Based_Model)
  + [Protein-Based Model](https://2017.igem.org/Team%3AOxford/Protein_Based_Model)
  + [Disease Model](https://2017.igem.org/Team%3AOxford/Disease_Model)
  + [Software](https://2017.igem.org/Team%3AOxford/Software)
* HUMAN PRACTICES
  + [Silver HP](https://2017.igem.org/Team%3AOxford/HP/Silver)
  + [Integrated and Gold](https://2017.igem.org/Team%3AOxford/HP/Gold_Integrated)
  + [Public Engagement](https://2017.igem.org/Team%3AOxford/Engagement)
* PEOPLE
  + [Meet the Team](https://2017.igem.org/Team%3AOxford/Team)
  + [Collaborations](https://2017.igem.org/Team%3AOxford/Collaborations)
  + [Attributions](https://2017.igem.org/Team%3AOxford/Attributions)
  + [Sponsors](https://2017.igem.org/Team%3AOxford/Sponsors)
  + [Our Awards](https://2017.igem.org/Team%3AOxford/Awards)

# Notebook

![](https://static.igem.org/mediawiki/2017/0/0f/Wet_lab_notebook.png)

## Introduction

We used the online resource Benchling to help keep an account and organise all the Wet Lab work that was conducted over the summer. We created a separate lab book for each major stage of the process, and each step carried out was linked to the protocol and tagged to the person who performed the task. This was helpful for tracking progress and troubleshooting.

[PCR and Gels](Notebook/PCRandGels#PRCandGels)

[Cloning and Growth](Notebook/CloningandGrowth#CloningandGrowth)

[Expression Vector-
Quick Change](Notebook/ExpressionVector#ExpressionVector)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)


[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Oxford/Experiments

[![](https://static.igem.org/mediawiki/2017/8/82/T--oxford--see--cruzi--logo--inverted.png)](https://2017.igem.org/Team%3AOxford)

* [HOME](https://2017.igem.org/Team%3AOxford)
* PROJECT
  + [Description](https://2017.igem.org/Team%3AOxford/Description)
  + [Chagas Disease](https://2017.igem.org/Team%3AOxford/Chagas_Disease)
  + [Chagas Public Policy](https://2017.igem.org/Team%3AOxford/Chagas_Public_Policy)
  + [Design](https://2017.igem.org/Team%3AOxford/Design)
  + [Parts](https://2017.igem.org/Team%3AOxford/Parts)
  + [Applied Design](https://2017.igem.org/Team%3AOxford/Applied_Design)
  + [--Developing our Design](https://2017.igem.org/Team%3AOxford/Applied_Design_Developing)
  + [--Our Solution](https://2017.igem.org/Team%3AOxford/Applied_Design_Solution)
  + [--Implementation](https://2017.igem.org/Team%3AOxford/Applied_Design_Implementation)
  + [Safety](https://2017.igem.org/Team%3AOxford/Safety)
  + [Cell Free Report](https://2017.igem.org/Team%3AOxford/Cell_Free_Report)
  + [Initial Ideas](https://2017.igem.org/Team%3AOxford/InitialIdeas)
* WET LAB
  + [Overview](https://2017.igem.org/Team%3AOxford/Overview_Wet_Lab)
  + [Results (Cloning)](https://2017.igem.org/Team%3AOxford/Cloning)
  + [Results (DNA-Based system)](https://2017.igem.org/Team%3AOxford/Results_DNA)
  + [Results (Protein-Based system)](https://2017.igem.org/Team%3AOxford/Results_Protein)
  + [Future Experiments](https://2017.igem.org/Team%3AOxford/Future_experiments)
  + [Protocols](https://2017.igem.org/Team%3AOxford/Protocols)
  + [Notebook](https://2017.igem.org/Team%3AOxford/Notebook)
  + [Interlab](https://2017.igem.org/Team%3AOxford/InterLab)
  + [Measurement](https://2017.igem.org/Team%3AOxford/Measurement)
* DRY LAB
  + [Overview](https://2017.igem.org/Team%3AOxford/Model)
  + [DNA-Based Model](https://2017.igem.org/Team%3AOxford/DNA_Based_Model)
  + [Protein-Based Model](https://2017.igem.org/Team%3AOxford/Protein_Based_Model)
  + [Disease Model](https://2017.igem.org/Team%3AOxford/Disease_Model)
  + [Software](https://2017.igem.org/Team%3AOxford/Software)
* HUMAN PRACTICES
  + [Silver HP](https://2017.igem.org/Team%3AOxford/HP/Silver)
  + [Integrated and Gold](https://2017.igem.org/Team%3AOxford/HP/Gold_Integrated)
  + [Public Engagement](https://2017.igem.org/Team%3AOxford/Engagement)
* PEOPLE
  + [Meet the Team](https://2017.igem.org/Team%3AOxford/Team)
  + [Collaborations](https://2017.igem.org/Team%3AOxford/Collaborations)
  + [Attributions](https://2017.igem.org/Team%3AOxford/Attributions)
  + [Sponsors](https://2017.igem.org/Team%3AOxford/Sponsors)
  + [Our Awards](https://2017.igem.org/Team%3AOxford/Awards)

<

# Experiments

Our experiments aren't here, we combined them with results [here](https://2017.igem.org/Team%3AOxford/Overview_Wet_Lab)
For future experiments click [here](https://2017.igem.org/Team%3AOxford/Future_experiments)

![](https://static.igem.org/mediawiki/2017/0/09/T--oxford--oxigemicon.png)

Sponsors

[![](https://static.igem.org/mediawiki/2017/5/5b/T--oxford--sponsors--Ox.png)](https://www.ox.ac.uk/students/fees-funding/assistance/hardship/vcf?wssl=1)
[![](https://static.igem.org/mediawiki/2017/4/46/T--oxford--sponsor--bbsrc.png)](https://www.bbsrc.ac.uk/)
[![](https://static.igem.org/mediawiki/2017/9/9e/T--oxford--sponsor--wellcome-trust.png)](https://wellcome.ac.uk/)
[![](https://static.igem.org/mediawiki/2017/b/b8/T--oxford--sponsor--seb.png)](https://www.sebiology.org/)
[![](https://static.igem.org/mediawiki/2017/a/aa/T--oxford--sponsor--microbiology-society.png)](https://www.microbiologysociety.org/)
[![](https://static.igem.org/mediawiki/2017/d/de/T--oxford--sponsor--biochemistry-dept.png)](http://www.bioch.ox.ac.uk/)
[![](https://static.igem.org/mediawiki/2017/3/3f/T--oxford--sponsors--DeptEng.png)](http://www.eng.ox.ac.uk/)
[![](https://static.igem.org/mediawiki/2017/9/9e/T--oxford--sponsors--DeptMed1.png)](https://www.medsci.ox.ac.uk/)
[![](https://static.igem.org/mediawiki/2017/a/aa/T--oxford--sponsors--DeptZoo.png)](https://www.zoo.ox.ac.uk/)
[![](https://static.igem.org/mediawiki/2017/c/c5/T--oxford--sponsor--twist-bioscience.png)](https://twistbioscience.com/)
[![](https://static.igem.org/mediawiki/2017/f/fd/T--oxford--sponsor--idt.png)](https://www.idtdna.com/site)
[![](https://static.igem.org/mediawiki/2017/f/f7/T--oxford--sponsor--snapgene.png)](http://www.snapgene.com/)
[![](https://static.igem.org/mediawiki/2017/9/94/T--oxford--sponsor--neb.png)](https://www.neb.com/)
[![](https://static.igem.org/mediawiki/2017/9/96/T--oxford--sponsor--eppendorf.png)](https://www.eppendorf.com/UK-en/)
[![](https://static.igem.org/mediawiki/2017/7/7d/T--oxford--sponsor--bmg-labtech.png)](https://www.bmglabtech.com/)
[![](https://static.igem.org/mediawiki/2017/9/96/T--oxford--sponsor--microscope-services.png)](https://www.microscopeservices.co.uk/)
[![](https://static.igem.org/mediawiki/2017/b/bf/T--oxford--sponsor--pembroke.png)](https://www.pmb.ox.ac.uk/finance-reports-rokos-2017)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Oxford/Results

[![](https://static.igem.org/mediawiki/2017/8/82/T--oxford--see--cruzi--logo--inverted.png)](https://2017.igem.org/Team%3AOxford)

* [HOME](https://2017.igem.org/Team%3AOxford)
* PROJECT
  + [Description](https://2017.igem.org/Team%3AOxford/Description)
  + [Chagas Disease](https://2017.igem.org/Team%3AOxford/Chagas_Disease)
  + [Chagas Public Policy](https://2017.igem.org/Team%3AOxford/Chagas_Public_Policy)
  + [Design](https://2017.igem.org/Team%3AOxford/Design)
  + [Parts](https://2017.igem.org/Team%3AOxford/Parts)
  + [Applied Design](https://2017.igem.org/Team%3AOxford/Applied_Design)
  + [--Developing our Design](https://2017.igem.org/Team%3AOxford/Applied_Design_Developing)
  + [--Our Solution](https://2017.igem.org/Team%3AOxford/Applied_Design_Solution)
  + [--Implementation](https://2017.igem.org/Team%3AOxford/Applied_Design_Implementation)
  + [Safety](https://2017.igem.org/Team%3AOxford/Safety)
  + [Cell Free Report](https://2017.igem.org/Team%3AOxford/Cell_Free_Report)
  + [Initial Ideas](https://2017.igem.org/Team%3AOxford/InitialIdeas)
* WET LAB
  + [Overview](https://2017.igem.org/Team%3AOxford/Overview_Wet_Lab)
  + [Results (Cloning)](https://2017.igem.org/Team%3AOxford/Cloning)
  + [Results (DNA-Based system)](https://2017.igem.org/Team%3AOxford/Results_DNA)
  + [Results (Protein-Based system)](https://2017.igem.org/Team%3AOxford/Results_Protein)
  + [Future Experiments](https://2017.igem.org/Team%3AOxford/Future_experiments)
  + [Protocols](https://2017.igem.org/Team%3AOxford/Protocols)
  + [Notebook](https://2017.igem.org/Team%3AOxford/Notebook)
  + [Interlab](https://2017.igem.org/Team%3AOxford/InterLab)
  + [Measurement](https://2017.igem.org/Team%3AOxford/Measurement)
* DRY LAB
  + [Overview](https://2017.igem.org/Team%3AOxford/Model)
  + [DNA-Based Model](https://2017.igem.org/Team%3AOxford/DNA_Based_Model)
  + [Protein-Based Model](https://2017.igem.org/Team%3AOxford/Protein_Based_Model)
  + [Disease Model](https://2017.igem.org/Team%3AOxford/Disease_Model)
  + [Software](https://2017.igem.org/Team%3AOxford/Software)
* HUMAN PRACTICES
  + [Silver HP](https://2017.igem.org/Team%3AOxford/HP/Silver)
  + [Integrated and Gold](https://2017.igem.org/Team%3AOxford/HP/Gold_Integrated)
  + [Public Engagement](https://2017.igem.org/Team%3AOxford/Engagement)
* PEOPLE
  + [Meet the Team](https://2017.igem.org/Team%3AOxford/Team)
  + [Collaborations](https://2017.igem.org/Team%3AOxford/Collaborations)
  + [Attributions](https://2017.igem.org/Team%3AOxford/Attributions)
  + [Sponsors](https://2017.igem.org/Team%3AOxford/Sponsors)
  + [Our Awards](https://2017.igem.org/Team%3AOxford/Awards)

# Results

Here you can describe the results of your project and your future plans.

##### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

##### You should also describe what your results mean:

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

##### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

##### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)