iGEM 2017 — Team XJTLU-CHINA

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:XJTLU-CHINA/Notebook

Toggle navigation

[![Brand](https://static.igem.org/mediawiki/2017/8/83/XJTLU_iGEM_logo.png)](https://2017.igem.org/Team%3AXJTLU-CHINA)

* [Home](https://2017.igem.org/Team%3AXJTLU-CHINA)
* Project
  + [Description](https://2017.igem.org/Team%3AXJTLU-CHINA/Description)
  + [Detection](https://2017.igem.org/Team%3AXJTLU-CHINA/Detection)
  + [Peptide Production](https://2017.igem.org/Team%3AXJTLU-CHINA/PeptideProduction)
  + [Cell Lysis](https://2017.igem.org/Team%3AXJTLU-CHINA/Lysis)
  + [Modeling](https://2017.igem.org/Team%3AXJTLU-CHINA/Model)
  + [Demonstration](https://2017.igem.org/Team%3AXJTLU-CHINA/Demonstrate)
  + [Application](https://2017.igem.org/Team%3AXJTLU-CHINA/Applied_Design)
* [Parts](https://2017.igem.org/Team%3AXJTLU-CHINA/Parts)
  + [Basic Parts](https://2017.igem.org/Team%3AXJTLU-CHINA/Basic_Part)
  + [Composite Parts](https://2017.igem.org/Team%3AXJTLU-CHINA/Composite_Part)
* [Notebook](https://2017.igem.org/Team%3AXJTLU-CHINA/Notebook)
  + [Lab Book](https://2017.igem.org/Team%3AXJTLU-CHINA/Notebook)
  + [Protocols](https://2017.igem.org/Team%3AXJTLU-CHINA/Protocols)
* Human Practice
  + [HP\_Gold](https://2017.igem.org/Team%3AXJTLU-CHINA/HP/Gold_Integrated)
  + [HP\_Silver](https://2017.igem.org/Team%3AXJTLU-CHINA/HP/Silver)
* [Team](https://2017.igem.org/Team%3AXJTLU-CHINA/Team)
  + [Members](https://2017.igem.org/Team%3AXJTLU-CHINA/Team)
  + [Attributions](https://2017.igem.org/Team%3AXJTLU-CHINA/Attributions)
  + [Collaboration](https://2017.igem.org/Team%3AXJTLU-CHINA/Collaborations)
* [Safety](https://2017.igem.org/Team%3AXJTLU-CHINA/Safety)
* [Interlab](https://2017.igem.org/Team%3AXJTLU-CHINA/InterLab)

Lab book

# Lab Book

# Molecular experiments notes of our project

## Experiments overview spanning the project

### June

6.21

1. Transformed plasmid pMG36e into *E.coli* Top10.

6.22

1. Transformed plasmid pUC57-DRGN-1 into *E.coli* Top10.
2. Grew pMG36e-transformed *E.coli* in LB broth.

6.23

1. Isolation of pMG36e plasmid from bacteria solution.
2. Grew DRGN-1 transformed *E.coli* in LB broth.
3. Ran agarose gel for pMG36e. Result: no target band.

6.24

1. Isolation of pUC57-DRGN-1 from bacteria solution.
2. Double-enzyme digestion of isolated pUC57-DRGN-1 using EcoRI and PstI.
3. Ran agarose gel for confirmation. Result: confirmed.

6.25

1. Repeated the amplification experiment of pMG36e.
2. Preparation of DH5ɑ competent cells.

6.26

1. Selected single colony of pMG36e-transformed *E. coli*, and transferred in LB broth.
2. Amplified the *E. coli* DH5ɑ containing pNZ8148 plasmid.

6.27

1. The extraction of pMG36e and pNZ8148 plasmid.
2. Digestion of pNZ8148 using XbaI.
3. Agarose gel electrophoresis of digested pNZ8148. Result: confirmed, and the extracted plasmid was in super-coiled form.

6.30

1. Gel electrophoresis for pMG36e, pUC57-DRGN-1 and pUC19. Results：no band for pMG36e, the other two were successful.

---

### July

7.18

1. Preparation of SGM17 broth, inoculation of *L. lactis* NZ9000 strain in medium.

7.19

1. Cultured another batch of *L. lactis* NZ9000 strain.

7.20

1. Preparation of *L. lactis* NZ9000 competent cells.

7.21

1. Preparation of *L. lactis* NZ9000 competent cells.

7.22

1. Electroporation of pUC57-3\*AMPs into *L. lactis* NZ9000 strain.
2. Electroporation of acmA into *L. lactis* NZ9000 strain.

7.23

1. Selected single colony of transformant and cultured in liquid medium for plasmid isolation.

7.24

1. Extraction of plasmid from transformant bacteria.
2. PCR the isolated plasmid using standard primers.
3. Agarose gel electrophoresis of PCR products. Result: no target band.

7.25

1. PCR the original pUC57-3\*AMP, pUC57-acmA and isolated plasmid using standard primers (for Gibson Assembly).
2. Agarose gel electrophoresis. Result: PCR products from the original plasmid had target band; the product from isolated plasmid had no target band.

7.26

1. Digestion of pNZ8148 using PstI.
2. Gel electrophoresis. Result: target band gained.

7.27

1. Gibson Assembly to integrate acmA in pNZ8148 and integrate 3\*AMP in pNZ8148.
2. Preparation of electroporation recovery solution.
3. Electroporation and cultured the transformant on selection plate.

7.28

1. Checked the transformant plate. Result: Two plates (one for bacteria transformed with pNZ8148-3\*AMPs and one with pNZ8148-acmA) had colonies but there were foreign bacteria; other plates either were much contaminated or didn’t have colonies at all.
2. Selected the single colony from plates and cultured them respectively in liquid media.

7.29

1. Checked the empty plate again. Result: still no bacteria grew.
2. Streaked the bacteria from liquid culture on plates to check and separate the *L. lactis* from foreign contamination.

7.30

1. Checked the result of streaking contaminated liquid transformant culture (bacteria containing acmA and 3\*AMPs).

---

### August

8.01

1. Made another batch of Chl-resistant M17 plates.
2. Took *L. lactis* NZ9000 strain from liquid culture (preserved culture) and streaked on M17 Chl-resistant plates to check whether the original bacteria was contaminated. Result: not contaminated.
3. Measured the CFU of competent *L. lactis*—dilution of the competent cells and spread on M17 plates.

8.02

1. Electroporation of competent *L. lactis* with empty vector pNZ8148, and spread on M17 plates.

8.03

1. Selection of single colony of transformant that contains pNZ8148, and grew in liquid M17 broth.

8.04

1. Plasmid pNZ8148 isolation from transformant cell culture.
2. Transformation of recombinants (pNZ8148-3\*AMPs and pNZ8148-acmA) into *E. coli* DH5ɑ.
3. PstI digestion of isolated pNZ8148 plasmid from *L. lactis*.
4. Agarose gel electrophoresis. Result: pNZ8148 confirmed.

8.05

1. Plasmid extraction of recombinants (pNZ8148-3\*AMPs and pNZ8148-acmA) from *E. coli* DH5ɑ.
2. PCR the extracts of experiment 1.
3. Gel electrophoresis. Result: target band confirmed.

8.08

1. Transformation of sfGFP in *E. coli* DH5ɑ.
2. Electroporation of *L. lactis* NZ9000 with pNZ8148.
3. PCR the AIP sensing device generator (BBa\_K2309003) from pUC57 vector.
4. Agarose gel electrophoresis. Result: no target band shown.

8.09

1. Selected single colonies and inoculated into LB broth.
2. PCR 3\*AMPs, acmA and AIP sensing device generator using standard primers.
3. Agarose gel electrophoresis. Result: no wanted band for each of the three products.
4. Inoculated the pNZ8148-transformed *L. lactis* in Chl-resistant M17 broth.

8.10

1. Extraction of pSB1C3-sfGFP from transformant *E. coli*.
2. PCR sfGFP from the extracted plasmid.
3. Agarose gel electrophoresis. Result: confirmed.
4. Isolation of pNZ8148 from transformant *L. lactis*.
5. Took Nanodrop measurement of DNA extracted. Result: no DNA.

8.11

1. New standard primers arrived. PCR 3\*AMPs, acmA and AIP sensing device.

8.13

1. Gel electrophoresis of PCR products. Results: target bands of three genes found.

8.15

1. Single enzyme digestion of pNZ8148 using PstI.

8.16

1. Agarose gel electrophoresis of digested pNZ8148 plasmid.
2. Gibson Assembly for linking 3\*AMPs and acmA to pNZ8148 respectively.

8.28

1. Selected single colony from previous *L. lactis* that were transformed with pNZ8148. Inoculated into Chl-resistant M17 broth.

8.29

1. Isolation of plasmid from *L. lactis*.
2. Using Nanodrop measured the concentration.
3. Gel electrophoresis.

---

### September

9.02

1. Preparation of solutions for *L. lactis* competent cells making.
2. Incubated *L. lactis* in M17 broth.

9.03

1. Made *L. lactis* competent cells.

9.04

1. Diluted the competent cells and inoculated on M17 plates.
2. Counted the colony number and calculated the CFU concentration of prepared competent cells.

9.05

1. Electroporation of *L. lactis* competent cells with empty vector pNZ8148.

9.06

1. Calculated the transformation efficiency. Result: 1.11\*104 CFU/ng.

9.09

1. Electroporation of *L. lactis* with pNZ8148-acmA and inoculated on Chl-resistant plates.

9.10

1. Selected the single colony from plates, and inoculated in liquid media.

9.11

1. Diluted the bacteria solution and put in microplate reader. Measured the successive OD600 for 1 day.

9.12

1. Induced the bacteria with nisin, and continued measuring the OD600 value for 2 days.

9.14

1. Observed the growth curve of nisin-induced bacteria.

9.18

1. Made Chl-resistant M17 broth.
2. Tested the chloramphenicol effectivity.

9.19

1. Digestion of pNZ8148 using PstI.
2. Cleaned up the prior PCR products.
3. Gibson Assembly.

9.20

1. Transformed acmA in *L. lactis* by electroporation.

9.21-9.24

Nisin-induced expression experiments (the same as the experiments done during 9.10-9.14).

9.25-10.10

Collaboration assignments.

---

### October

10.11

1. Amplification of linearized pSB1C3.
2. Gel electrophoresis.
3. PCR products purification.

10.12

1. PCR the LL-37-His-tag and GF-17 gene.
2. Gel electrophoresis.

10.13

1. PCR products purification.
2. Digestion of linearized pSB1C3 using EcoRI and PstI.

10.16

1. Double enzyme digestion of the LL-37-His-tag and GF-17 gene using EcoRI and PstI.
2. Ligated LL-37-His-tag with digested pSB1C3, and GF-17 with pSB1C3.

10.17

1. Constructed Biobrick tandem repeats 3 xAMPs on plasmid pSB1C3.
2. Constructed Biobrick AcmA on plasmid pSB1C3.
3. Constructed Biobrick Quorum seneing (QS) on plasmid pSB1C3.
4. Agarose gel electrophoresis.

10.18

1. Constructed Biobrick tandem repeats 3x AMP on plasmid pEt 28a (+).
2. Constructed Biobrick LL-37+ 6x His tag on plasmid pEt 28a (+).
3. Agarose gel electrophoresis.

10.20

1. Induced LL-37+ 6x His tag protein expression with IPTG.
2. Dot blot.

---

# Anti-microbial peptides assays notes of our project

### June

6.20

1. Tested DRGN-1's effect on *Escherichia coli* (DH5α).

6.23

1. Tested DRGN-1's effect on *Lactobacillus rhamnosus* (LGG).

6.25

1. Tested DRGN-1's effect on *Pseudomonas fluorescens*.

6.28

1. Tested DRGN-1's effect on *Escherichia coli* DH5α, *Staphylococcus aureus*, and *Pseudomonas fluorescens*
   with overnight culture in 37℃ and 200rpm.

6.29

1. Prepared DRGN-1 solution gradients: 25ug/ml, 50ug/ml, 100ug/ml, 200ug/ml.
2. Prepared antibiotic (chloramphenicol) solution gradients: 25ug/ml, 50ug/ml.
3. Prepared *Escherichia coli* DH5α/ *Staphylococcus aureus* /*Pseudomonas fluorescens* solution gradients: x100, x1000.

6.30

Result:

1. DRGN-1 has effects on *Staphylococcus aureus*.
2. DRGN-1 has no effect on *E. coli* DH5α and *Pseudomonas fluorescens* strain.

---

### July

7.01

Inhibition Ring of LL-37 on *Staphylococcus aureus*

1. Cultured *Staphylococcus aureus* in 37℃, 200rpm and for 8 hours.
2. Added LL-37 solution to discs.
3. Added *Staphylococcus aureus* to LB agar plates.

7.04

Inhibition Ring of Grammistin-Pp1 on *Staphylococcus aureus*

1. Cultured *Staphylococcus aureus* in 37℃, 200rpm and for 8 hours.
2. Added Grammistin-Pp1 solution to discs.
3. Added *Staphylococcus aureus* to LB agar plates.

7.07

Inhibition Ring of GF-17 on *Staphylococcus aureus*

1. Cultured *Staphylococcus aureus*in 37℃, 200rpm and for 8 hours.
2. Added GF-17 solution to discs.
3. Added *Staphylococcus aureus* to LB agar plates.

7.10

Inhibition Ring of the mixed AMPs on *Staphylococcus aureus*

1. Cultured *Staphylococcus aureus*in 37℃, 200rpm and for 8 hours.
2. Added the mixed AMPs solution to discs.
3. Added *Staphylococcus aureus* to LB agar plates.

7.13

Minimal Inhibitory Concentration (MIC) assay of LL-37 on *Staphylococcus aureus*

1. Cultured *Staphylococcus aureus*in 37℃, 200rpm and for 8 hours.
2. Pipetted same volume of *Staphylococcus aureus* and the diluted LL-37 solution and mixed.
3. Incubated overnight.

7.15

Minimal Inhibitory Concentration (MIC) assay of Grammistin-Pp1 on *Staphylococcus aureus*

1. Cultured *Staphylococcus aureus* in 37℃, 200rpm and for 8 hours.
2. Pipetted same volume of *Staphylococcus aureus* and the diluted Grammistin-Pp1 solution and mixed.
3. Incubated overnight.

7.17

Minimal Inhibitory Concentration (MIC) assay of GF-17 on *Staphylococcus aureus*

1. Cultured *Staphylococcus aureus* in 37℃, 200rpm and for 8 hours.
2. Pipetted same volume of *Staphylococcus aureus* and the the diluted Grammistin-Pp1 solution and mixed.
3. Incubated overnight.

7.19

Minimal Inhibitory Concentration (MIC) assay of the mixed AMPs on *Staphylococcus aureus*

1. Cultured *Staphylococcus aureus* in 37℃, 200rpm and for 8 hours.
2. Pipetted same volume of *Staphylococcus aureus* and the diluted AMPs mixture solution and mixed.
3. Incubated overnight.

7.22

1. Anti-biofilm assay of LL-37 on *Staphylococcus aureus*.

7.25

1. Anti-biofilm assay of Grammistin-Pp1 on *Staphylococcus aureus*.

7.29

1. Anti-biofilm assay of GF-17 on *Staphylococcus aureus*.

---

### August

8.01

1. Anti-biofilm assay of the mixed AMPs on *Staphylococcus aureus* biofilms.

8.06

1. Growth curve measure of *Staphylococcus aureus*.

8.09

1. Growth curve measure of *Staphylococcus aureus* under the effect of the peptide LL-37.

8.12

1. Growth curve measure of *Staphylococcus aureus* under the effect of the peptide Grammistin-Pp1.

8.16

1. Growth curve measure of *Staphylococcus aureus* under the effect of the peptide GF-17.

8.19

1. Growth curve measure of *Staphylococcus aureus* under the effect of the mixed AMPs.

---

# Interlab experiments

### July

7.10

1. Prepared SOC medium and LB agar plates with chloramphenicol.

7.15

1. Calibrated the spectrophotometer and constructed the fluorescein calibration curve.

7.16

1. Tested competent cells.

7.17

1. Too many bacteria, made new competent cells.

7.19

1. Tested competent cells again.

7.20

1. Ordered new chloramphenicol.

---

### August

8.1

1. Tested competent cells again and compared the newly arrived chloramphenicol with the old one.

8.2

1. Negative results.

8.3

1. Tested the effects of chloramphenicol with the concentration ranging from 1-50 μg/ml.

8.4

1. Negative results.
2. Tested the effects of chloramphenicol with the concentration ranging from 50-200 μg/ml.

8.5

1. Negative results.

8.7

1. Tested the borrowed chloramphenicol.

8.8

1. Positive results. Tested competent cells.

8.9

1. Positive results.
2. Transformation.

8.10

1. Inoculated bacteria to the liquid medium.

8.11

1. Diluted bacteria and measure the OD600 by using the spectrophotometer, and measured fluorescence by using plate reader.

# Collaborators and Supporters

[![](https://static.igem.org/mediawiki/2017/3/38/Synbio_tech_logo.png)](https://www.synbio-tech.com.cn)

[![](https://static.igem.org/mediawiki/2017/a/ab/Wuxi_No.2_people%27s_hospital.png)](https://www.wx2h.com/web/index.php)

[![](https://static.igem.org/mediawiki/2017/e/e8/Qiang_yao_sheng_wu.png)](https://www.chinapeptides.qianyan.biz)

[![](https://static.igem.org/mediawiki/2017/0/06/NEB_logo.png)](https://www.neb.com)

[![](https://static.igem.org/mediawiki/2017/c/cb/Snapgene_logo.png)](https://www.snapgene.com)

[![](https://static.igem.org/mediawiki/2017/9/9b/Genscript.png)](https://www.genscript.com)

#### Location

Rm 363, Science Building
Xi'an Jiaotong-Liverpool University
111 Ren'ai Road, Suzhou, China
215123

#### Social

![facebook](https://static.igem.org/mediawiki/2017/9/9f/XJTLU_facebook.png)
![blog](https://static.igem.org/mediawiki/2017/7/72/XJTLU_blog.png)

#### Get in touch

![emali](https://static.igem.org/mediawiki/2017/1/19/XJTLU_email.png)

igem@xjtlu.edu.cn

XJTLU-CHINA iGEM 2017


[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:XJTLU-CHINA/Experiments

Toggle navigation

[![Brand](https://static.igem.org/mediawiki/2017/8/83/XJTLU_iGEM_logo.png)](https://2017.igem.org/Team%3AXJTLU-CHINA)

* [Home](https://2017.igem.org/Team%3AXJTLU-CHINA)
* Project
  + [Description](https://2017.igem.org/Team%3AXJTLU-CHINA/Description)
  + [Detection](https://2017.igem.org/Team%3AXJTLU-CHINA/Detection)
  + [Peptide Production](https://2017.igem.org/Team%3AXJTLU-CHINA/PeptideProduction)
  + [Cell Lysis](https://2017.igem.org/Team%3AXJTLU-CHINA/Lysis)
  + [Modeling](https://2017.igem.org/Team%3AXJTLU-CHINA/Model)
  + [Demonstration](https://2017.igem.org/Team%3AXJTLU-CHINA/Demonstrate)
  + [Application](https://2017.igem.org/Team%3AXJTLU-CHINA/Applied_Design)
* [Parts](https://2017.igem.org/Team%3AXJTLU-CHINA/Parts)
  + [Basic Parts](https://2017.igem.org/Team%3AXJTLU-CHINA/Basic_Part)
  + [Composite Parts](https://2017.igem.org/Team%3AXJTLU-CHINA/Composite_Part)
* [Notebook](https://2017.igem.org/Team%3AXJTLU-CHINA/Notebook)
  + [Lab Book](https://2017.igem.org/Team%3AXJTLU-CHINA/Notebook)
  + [Protocols](https://2017.igem.org/Team%3AXJTLU-CHINA/Protocols)
* Human Practice
  + [HP\_Gold](https://2017.igem.org/Team%3AXJTLU-CHINA/HP/Gold_Integrated)
  + [HP\_Silver](https://2017.igem.org/Team%3AXJTLU-CHINA/HP/Silver)
* [Team](https://2017.igem.org/Team%3AXJTLU-CHINA/Team)
  + [Members](https://2017.igem.org/Team%3AXJTLU-CHINA/Team)
  + [Attributions](https://2017.igem.org/Team%3AXJTLU-CHINA/Attributions)
  + [Collaboration](https://2017.igem.org/Team%3AXJTLU-CHINA/Collaborations)
* [Safety](https://2017.igem.org/Team%3AXJTLU-CHINA/Safety)
* [Interlab](https://2017.igem.org/Team%3AXJTLU-CHINA/InterLab)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

##### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

##### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:XJTLU-CHINA/Results

Toggle navigation

[![Brand](https://static.igem.org/mediawiki/2017/8/83/XJTLU_iGEM_logo.png)](https://2017.igem.org/Team%3AXJTLU-CHINA)

* [Home](https://2017.igem.org/Team%3AXJTLU-CHINA)
* Project
  + [Description](https://2017.igem.org/Team%3AXJTLU-CHINA/Description)
  + [Detection](https://2017.igem.org/Team%3AXJTLU-CHINA/Detection)
  + [Peptide Production](https://2017.igem.org/Team%3AXJTLU-CHINA/PeptideProduction)
  + [Cell Lysis](https://2017.igem.org/Team%3AXJTLU-CHINA/Lysis)
  + [Modeling](https://2017.igem.org/Team%3AXJTLU-CHINA/Model)
  + [Demonstration](https://2017.igem.org/Team%3AXJTLU-CHINA/Demonstrate)
  + [Application](https://2017.igem.org/Team%3AXJTLU-CHINA/Applied_Design)
* [Parts](https://2017.igem.org/Team%3AXJTLU-CHINA/Parts)
  + [Basic Parts](https://2017.igem.org/Team%3AXJTLU-CHINA/Basic_Part)
  + [Composite Parts](https://2017.igem.org/Team%3AXJTLU-CHINA/Composite_Part)
* [Notebook](https://2017.igem.org/Team%3AXJTLU-CHINA/Notebook)
  + [Lab Book](https://2017.igem.org/Team%3AXJTLU-CHINA/Notebook)
  + [Protocols](https://2017.igem.org/Team%3AXJTLU-CHINA/Protocols)
* Human Practice
  + [HP\_Gold](https://2017.igem.org/Team%3AXJTLU-CHINA/HP/Gold_Integrated)
  + [HP\_Silver](https://2017.igem.org/Team%3AXJTLU-CHINA/HP/Silver)
* [Team](https://2017.igem.org/Team%3AXJTLU-CHINA/Team)
  + [Members](https://2017.igem.org/Team%3AXJTLU-CHINA/Team)
  + [Attributions](https://2017.igem.org/Team%3AXJTLU-CHINA/Attributions)
  + [Collaboration](https://2017.igem.org/Team%3AXJTLU-CHINA/Collaborations)
* [Safety](https://2017.igem.org/Team%3AXJTLU-CHINA/Safety)
* [Interlab](https://2017.igem.org/Team%3AXJTLU-CHINA/InterLab)

# Results

Here you can describe the results of your project and your future plans.

##### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

##### You should also describe what your results mean:

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

##### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

##### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)