iGEM 2009 — Team Uppsala-Sweden

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# Team:Uppsala-Sweden/Notebook

### From 2009.igem.org

![](https://static.igem.org/mediawiki/2009/3/32/Uppsala_header.png)

* [Home](https://2009.igem.org/Team%3AUppsala-Sweden)
* [Vision](https://2009.igem.org/Team%3AUppsala-Sweden/Vision)
* Project

  [Butanol](https://2009.igem.org/Team%3AUppsala-Sweden/Butanol)
  [Ethanol](https://2009.igem.org/Team%3AUppsala-Sweden/Ethanol)
  [Pir Proteins](https://2009.igem.org/Team%3AUppsala-Sweden/pir)
  [Antisense RNA](https://2009.igem.org/Team%3AUppsala-Sweden/rna)
* Modelling

  [Modelling of ethanol production](https://2009.igem.org/Team%3AUppsala-Sweden/Modelling)
  [PLS model of ethanol concentration](https://2009.igem.org/Team%3AUppsala-Sweden/PLS)
* [Parts](https://2009.igem.org/Team%3AUppsala-Sweden/Parts)

  [Promoter](https://2009.igem.org/Team%3AUppsala-Sweden/Parts#pro)
  [Butanol](https://2009.igem.org/Team%3AUppsala-Sweden/Parts#But)
  [Ethanol 1](https://2009.igem.org/Team%3AUppsala-Sweden/Parts#eth1)
  [Ethanol 2](https://2009.igem.org/Team%3AUppsala-Sweden/Parts#eth2)
  [Pir](https://2009.igem.org/Team%3AUppsala-Sweden/Parts#pir)
  [Antisense RNA](https://2009.igem.org/Team%3AUppsala-Sweden/Parts#rna)
* [Team](https://2009.igem.org/Team%3AUppsala-Sweden/Team)

  [About the Team](https://2009.igem.org/Team%3AUppsala-Sweden/Team#About_the_Team)
  [Students](https://2009.igem.org/Team%3AUppsala-Sweden/Team#Students)
  [Advisors](https://2009.igem.org/Team%3AUppsala-Sweden/Team#Advisors)
* [Notebook](https://2009.igem.org/Team%3AUppsala-Sweden/Notebook)
* [Acknowledgements](https://2009.igem.org/Team%3AUppsala-Sweden/Acknowledgements)

  [Acknowledgements](https://2009.igem.org/Team%3AUppsala-Sweden/Acknowledgements#Acknowledgements)
  [Sponsors](https://2009.igem.org/Team%3AUppsala-Sweden/Acknowledgements#Sponsors)

## Notebook

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| |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | June | | | | | | | | M | T | W | T | F | S | S | | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/1\_June\_2009&action=edit 1] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/2\_June\_2009&action=edit 2] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/3\_June\_2009&action=edit 3] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/4\_June\_2009&action=edit 4] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/5\_June\_2009&action=edit 5] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/6\_June\_2009&action=edit 6] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/7\_June\_2009&action=edit 7] | | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/8\_June\_2009&action=edit 8] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/9\_June\_2009&action=edit 9] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/10\_June\_2009&action=edit 10] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/11\_June\_2009&action=edit 11] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/12\_June\_2009&action=edit 12] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/13\_June\_2009&action=edit 13] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/14\_June\_2009&action=edit 14] | | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/15\_June\_2009&action=edit 15] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/16\_June\_2009&action=edit 16] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/17\_June\_2009&action=edit 17] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/18\_June\_2009&action=edit 18] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/19\_June\_2009&action=edit 19] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/20\_June\_2009&action=edit 20] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/21\_June\_2009&action=edit 21] | | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/22\_June\_2009&action=edit 22] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/23\_June\_2009&action=edit 23] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/24\_June\_2009&action=edit 24] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/25\_June\_2009&action=edit 25] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/26\_June\_2009&action=edit 26] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/27\_June\_2009&action=edit 27] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/28\_June\_2009&action=edit 28] | | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/29\_June\_2009&action=edit 29] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/30\_June\_2009&action=edit 30] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | July | | | | | | | | M | T | W | T | F | S | S | |  |  | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/1\_July\_2009&action=edit 1] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/2\_July\_2009&action=edit 2] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/3\_July\_2009&action=edit 3] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/4\_July\_2009&action=edit 4] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/5\_July\_2009&action=edit 5] | | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/6\_July\_2009&action=edit 6] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/7\_July\_2009&action=edit 7] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/8\_July\_2009&action=edit 8] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/9\_July\_2009&action=edit 9] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/10\_July\_2009&action=edit 10] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/11\_July\_2009&action=edit 11] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/12\_July\_2009&action=edit 12] | | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/13\_July\_2009&action=edit 13] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/14\_July\_2009&action=edit 14] | [http://2009.igem.org/Uppsala-Sweden/15\_July\_2009 15] | [http://2009.igem.org/Uppsala-Sweden/16\_July\_2009 16] | [http://2009.igem.org/Uppsala-Sweden/17\_July\_2009 17] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/18\_July\_2009&action=edit 18] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/19\_July\_2009&action=edit 19] | | [http://2009.igem.org/Uppsala-Sweden/20\_July\_2009 20] | [http://2009.igem.org/Uppsala-Sweden/21\_July\_2009 21] | [http://2009.igem.org/Uppsala-Sweden/22\_July\_2009 22] | [http://2009.igem.org/Uppsala-Sweden/23\_July\_2009 23] | [http://2009.igem.org/Uppsala-Sweden/24\_July\_2009 24] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/25\_July\_2009&action=edit 25] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/26\_July\_2009&action=edit 26] | | [http://2009.igem.org/Uppsala-Sweden/27\_July\_2009 27] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/28\_July\_2009&action=edit 28] | [http://2009.igem.org/Uppsala-Sweden/29\_July\_2009 29] | [http://2009.igem.org/Uppsala-Sweden/30\_July\_2009 30] | [http://2009.igem.org/Uppsala-Sweden/31\_July\_2009 31] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | August | | | | | | | | M | T | W | T | F | S | S | |  |  |  |  |  | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/1\_August\_2009&action=edit 1] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/2\_August\_2009&action=edit 2] | | [http://2009.igem.org/Uppsala-Sweden/3\_August\_2009 3] | [http://2009.igem.org/Uppsala-Sweden/4\_August\_2009 4] | [http://2009.igem.org/Uppsala-Sweden/5\_August\_2009 5] | [http://2009.igem.org/Uppsala-Sweden/6\_August\_2009 6] | [http://2009.igem.org/Uppsala-Sweden/7\_August\_2009 7] | [http://2009.igem.org/Uppsala-Sweden/8\_August\_2009 8] | [http://2009.igem.org/Uppsala-Sweden/9\_August\_2009 9] | | [http://2009.igem.org/Uppsala-Sweden/10\_August\_2009 10] | [http://2009.igem.org/Uppsala-Sweden/11\_August\_2009 11] | [http://2009.igem.org/Uppsala-Sweden/12\_August\_2009 12] | [http://2009.igem.org/Uppsala-Sweden/13\_August\_2009 13] | [http://2009.igem.org/Uppsala-Sweden/14\_August\_2009 14] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/15\_August\_2009&action=edit 15] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/16\_August\_2009&action=edit 16] | | [http://2009.igem.org/Uppsala-Sweden/17\_August\_2009 17] | [http://2009.igem.org/Uppsala-Sweden/18\_August\_2009 18] | [http://2009.igem.org/Uppsala-Sweden/19\_August\_2009 19] | [http://2009.igem.org/Uppsala-Sweden/20\_August\_2009 20] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/21\_August\_2009&action=edit 21] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/22\_August\_2009&action=edit 22] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/23\_August\_2009&action=edit 23] | | [http://2009.igem.org/Uppsala-Sweden/24\_August\_2009 24] | [http://2009.igem.org/Uppsala-Sweden/25\_August\_2009 25] | [http://2009.igem.org/Uppsala-Sweden/26\_August\_2009 26] | [http://2009.igem.org/Uppsala-Sweden/27\_August\_2009 27] | [http://2009.igem.org/Uppsala-Sweden/28\_August\_2009 28] | [http://2009.igem.org/Uppsala-Sweden/29\_August\_2009 29] | [http://2009.igem.org/Uppsala-Sweden/30\_August\_2009 30] | | [http://2009.igem.org/Uppsala-Sweden/31\_August\_2009 31] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | September | | | | | | | | M | T | W | T | F | S | S | |  | [http://2009.igem.org/Uppsala-Sweden/1\_September\_2009 1] | [http://2009.igem.org/Uppsala-Sweden/2\_September\_2009 2] | [http://2009.igem.org/Uppsala-Sweden/3\_September\_2009 3] | [http://2009.igem.org/Uppsala-Sweden/4\_September\_2009 4] | [http://2009.igem.org/Uppsala-Sweden/5\_September\_2009 5] | [http://2009.igem.org/Uppsala-Sweden/6\_September\_2009 6] | | [http://2009.igem.org/Uppsala-Sweden/7\_September\_2009 7] | [http://2009.igem.org/Uppsala-Sweden/8\_September\_2009 8] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/9\_September\_2009&action=edit 9] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/10\_September\_2009&action=edit 10] | [http://2009.igem.org/Uppsala-Sweden/11\_September\_2009 11] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/12\_September\_2009&action=edit 12] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/13\_September\_2009&action=edit 13] | | [http://2009.igem.org/Uppsala-Sweden/14\_September\_2009 14] | [http://2009.igem.org/Uppsala-Sweden/15\_September\_2009 15] | [http://2009.igem.org/Uppsala-Sweden/16\_September\_2009 16] | [http://2009.igem.org/Uppsala-Sweden/17\_September\_2009 17] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/18\_September\_2009&action=edit 18] | [http://2009.igem.org/Uppsala-Sweden/19\_September\_2009 19] | [http://2009.igem.org/Uppsala-Sweden/20\_September\_2009 20] | | [http://2009.igem.org/Uppsala-Sweden/21\_September\_2009 21] | [http://2009.igem.org/Uppsala-Sweden/22\_September\_2009 22] | [http://2009.igem.org/Uppsala-Sweden/23\_September\_2009 23] | [http://2009.igem.org/Uppsala-Sweden/24\_September\_2009 24] | [http://2009.igem.org/Uppsala-Sweden/25\_September\_2009 25] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/26\_September\_2009&action=edit 26] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/27\_September\_2009&action=edit 27] | | [http://2009.igem.org/Uppsala-Sweden/28\_September\_2009 28] | [http://2009.igem.org/Uppsala-Sweden/29\_September\_2009 29] | [http://2009.igem.org/Uppsala-Sweden/30\_September\_2009 30] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | October | | | | | | | | M | T | W | T | F | S | S | |  |  |  | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/1\_October\_2009&action=edit 1] | [http://2009.igem.org/Uppsala-Sweden/2\_October\_2009 2] | [http://2009.igem.org/Uppsala-Sweden/3\_October\_2009 3] | [http://2009.igem.org/Uppsala-Sweden/4\_October\_2009 4] | | [http://2009.igem.org/Uppsala-Sweden/5\_October\_2009 5] | [http://2009.igem.org/Uppsala-Sweden/6\_October\_2009 6] | [http://2009.igem.org/Uppsala-Sweden/7\_October\_2009 7] | [http://2009.igem.org/Uppsala-Sweden/8\_October\_2009 8] | [http://2009.igem.org/Uppsala-Sweden/9\_October\_2009 9] | [http://2009.igem.org/Uppsala-Sweden/10\_October\_2009 10] | [http://2009.igem.org/Uppsala-Sweden/11\_October\_2009 11] | | [http://2009.igem.org/Uppsala-Sweden/12\_October\_2009 12] | [http://2009.igem.org/Uppsala-Sweden/13\_October\_2009 13] | [http://2009.igem.org/Uppsala-Sweden/14\_October\_2009 14] | [http://2009.igem.org/Uppsala-Sweden/15\_October\_2009 15] | [http://2009.igem.org/Uppsala-Sweden/16\_October\_2009 16] | [http://2009.igem.org/Uppsala-Sweden/17\_October\_2009 17] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/18\_October\_2009&action=edit 18] | | [http://2009.igem.org/Uppsala-Sweden/19\_October\_2009 19] | [http://2009.igem.org/Uppsala-Sweden/20\_October\_2009 20] | [http://2009.igem.org/Uppsala-Sweden/21\_October\_2009 21] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/22\_October\_2009&action=edit 22] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/23\_October\_2009&action=edit 23] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/24\_October\_2009&action=edit 24] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/25\_October\_2009&action=edit 25] | | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/26\_October\_2009&action=edit 26] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/27\_October\_2009&action=edit 27] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/28\_October\_2009&action=edit 28] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/29\_October\_2009&action=edit 29] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/30\_October\_2009&action=edit 30] | [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/31\_October\_2009&action=edit 31] | |

## Oligonucleotides used by the Uppsala iGEM Team 2009

All primers we used during the project can be found here: [iGEMUppsala09\_Primer.xls](/wiki/images/c/c8/IGEMUppsala09_Primer.xls "IGEMUppsala09 Primer.xls")

## Protocols

#### PCR Setup

[PCR MM and Primers.xls](/wiki/images/3/30/PCR_MM_and_Primers.xls "PCR MM and Primers.xls")

#### Transformation Protocol

[Transformation workorder.pdf](/wiki/images/a/ad/Transformation_workorder.pdf "Transformation workorder.pdf")

#### Growth media for *Z. mobilis*

[Fullmedium mobilis.xls](/wiki/images/9/9e/Fullmedium_mobilis.xls "Fullmedium mobilis.xls")

#### Growth medium for *Synechocystis sp PCC 6803*

[BG11.pdf](/wiki/images/6/69/BG11.pdf "BG11.pdf")

#### Growth medium for *E. coli*

[LB.pdf](/wiki/images/d/d7/LB.pdf "LB.pdf")

#### Various

[Glycerol Stocks.xls](/wiki/images/8/87/Glycerol_Stocks.xls "Glycerol Stocks.xls")
[Triparental\_Mating.pdf](/wiki/images/b/bf/Triparental_Mating.pdf "Triparental Mating.pdf")

---

[![Bioneer](http://aboutgmos.org/iGEM/logos/Bioneer_Logo.jpg)](http://www.bioneer.com)
[![Biolegio](http://aboutgmos.org/iGEM/logos/Biolegio_Logo.jpg)](http://www.biolegio.com)
[![Clontech](http://aboutgmos.org/iGEM/logos/Clontech_Logo.jpg)](http://www.clontech.com)
[![Uppsala Genome Center](http://aboutgmos.org/iGEM/logos/uppsalagenome_logo.jpg)](http://www.genomecenter.uu.se)

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