iGEM 2015 — Team IONIS_Paris

# Team:IONIS Paris/Notebook

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  + [Description](https://2015.igem.org/Team%3AIONIS_Paris/Description)
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Notebook

# Notebook

##

# MARCH 2015

# APRIL 2015

# MAY 2015

# JUNE 2015

# JULY 2015

# AUGUST 2015

# SEPT. 2015

#### Results after PCR from 17/09

##### Expected results

![Expected results](https://static.igem.org/mediawiki/2015/2/2e/Expectedresultats-180915.png)

##### Results

![Results](https://static.igem.org/mediawiki/2015/0/0d/Resultats-180915.png)

The band for the third well is good. WE GOT IT !!!!!

![img01](https://static.igem.org/mediawiki/2015/b/b8/Graph-180915.png)

Induction of BBa\_K325909 and BBa\_K1159001 with a solution of arabinose 1%, and a solution of IPTG 1 mM for T7-Nanoluc (should correspond to the effect of a constitutive promoter like T7). Under these conditions, light intensity of T7-nanoluc was 1,2 time higher than the BBa\_K1159001.

![img01](https://static.igem.org/mediawiki/2015/a/aa/Graph-180915_2.png)

Effect of the concentration of IPTG inducer to the light intensity of T7-nanoluc part. While the concentration of IPTG does not exceed 1 mM, the light intensity remains low.

![img01](https://static.igem.org/mediawiki/2015/8/80/Graph-180915_3.png)

Effect of the concentration of arabinose inducer to the light intensity of BBa\_K1159001 part. Light intensity is linked to inducer concentration however over 1% of arabinose (final concentration), the intensity not rise anymore.

18 Sept. 15