iGEM 2011 — Team UEA-JIC_Norwich

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# Team:UEA-JIC Norwich/Notebook

### From 2011.igem.org

University of East Anglia-JIC

UNIVERSITY OF EAST ANGLIA-JOHN INNES CENTRE

![](https://static.igem.org/mediawiki/2011/0/03/Ziggurats_for_banner.jpg)

![](https://static.igem.org/mediawiki/2011/d/db/JIC_AERIAL_VIEW_BANNER.jpg)

![](https://static.igem.org/mediawiki/2011/0/09/CHLAMY_BANNER.jpg)

![](https://static.igem.org/mediawiki/2011/d/db/Fountain_border.jpg)

![](https://static.igem.org/mediawiki/2011/0/05/Physcomitrella_P.jpg )

* [Home](https://2011.igem.org/Team%3AUEA-JIC_Norwich)
* [Team](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Team)

   [Students](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Team) |
   [Advisors](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Our_advisors) |
   [Acknowledgements](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Acknowledgements) |
* [Project](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Project)

   [Project Overview](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Project) |
   [Algae](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Nittygritty-algae) |
   [Moss](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Nittygritty-moss) |
   [Methods](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Methods) |
   [Lab Safety](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Safety) |
   [Attributions](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Attributions) |
* [Results](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Registryoverview)

   [Registry Overview](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Registryoverview)|
   [Data](https://2011.igem.org/Team%3AUEA-JIC_Norwich/data)|
* [Human Practice](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Human_practices/Interviews)

   [Interviews](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Human_practices/Interviews) |
   [Outreach](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Human_practices) |
   [UK Team Meetup](https://2011.igem.org/Team%3AUEA-JIC_Norwich/UKConference) |
   [Media](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Human_practices/Media) |
   [School Work](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Human_practices/schools) |
  * [Lab Journal](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Lab%20Journal)

    [Week 1](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weekone) |
    [Week 2](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weektwo) |
    [Week 3](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weekthree) |
    [Week 4](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weekfour) |
    [Week 5](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weekfive) |
    [Week 6](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weeksix) |
    [Week 7](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weekseven) |
    [Week 8](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weekeight) |
    [Week 9](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weeknine) |
    [Week 10](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weekten) |
    [Week 11](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weekeleven) |
    [Week 12](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weektwelve) |
    [Week 13](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weekthirteen) |
    [Week 14](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weekfourteen) |
    [Week 15](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Weekfifteen) |
* [Software](https://2011.igem.org/Team%3AUEA-JIC_Norwich/software)
* [Photo Gallery](https://2011.igem.org/Team%3AUEA-JIC_Norwich/gallery)
* [Sponsors](https://2011.igem.org/Team%3AUEA-JIC_Norwich/Our_Sponsors)

# LAB JOURNAL

We began our Laboratory work on Monday the 20th of June. Click on a date below to see the wet work we did that day. If indicated, select the 'Methods' tab to view the protocol we followed to conduct our research. Enjoy!

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| |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | June | | | | | | | | M | T | W | T | F | S | S | |  |  | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/1\_June\_2011&action=edit 1] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/2\_June\_2011&action=edit 2] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/3\_June\_2011&action=edit 3] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/4\_June\_2011&action=edit 4] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/5\_June\_2011&action=edit 5] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/6\_June\_2011&action=edit 6] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/7\_June\_2011&action=edit 7] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/8\_June\_2011&action=edit 8] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/9\_June\_2011&action=edit 9] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/10\_June\_2011&action=edit 10] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/11\_June\_2011&action=edit 11] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/12\_June\_2011&action=edit 12] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/13\_June\_2011&action=edit 13] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/14\_June\_2011&action=edit 14] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/15\_June\_2011&action=edit 15] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/16\_June\_2011&action=edit 16] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/17\_June\_2011&action=edit 17] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/18\_June\_2011&action=edit 18] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/19\_June\_2011&action=edit 19] | | [http://2011.igem.org/Team:UEA-JIC/Lab-entry/20\_June\_2011 20] | [http://2011.igem.org/Team:UEA-JIC/Lab-entry/21\_June\_2011 21] | [http://2011.igem.org/Team:UEA-JIC/Lab-entry/22\_June\_2011 22] | [http://2011.igem.org/Team:UEA-JIC/Lab-entry/23\_June\_2011 23] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/24\_June\_2011&action=edit 24] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/25\_June\_2011&action=edit 25] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/26\_June\_2011&action=edit 26] | | [http://2011.igem.org/Team:UEA-JIC/Lab-entry/27\_June\_2011 27] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/28\_June\_2011&action=edit 28] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/29\_June\_2011&action=edit 29] | [http://2011.igem.org/Team:UEA-JIC/Lab-entry/30\_June\_2011 30] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | July | | | | | | | | M | T | W | T | F | S | S | |  |  |  |  | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/1\_July\_2011&action=edit 1] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/2\_July\_2011&action=edit 2] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/3\_July\_2011&action=edit 3] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/4\_July\_2011&action=edit 4] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/5\_July\_2011&action=edit 5] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/6\_July\_2011&action=edit 6] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/7\_July\_2011&action=edit 7] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/8\_July\_2011&action=edit 8] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/9\_July\_2011&action=edit 9] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/10\_July\_2011&action=edit 10] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/11\_July\_2011&action=edit 11] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/12\_July\_2011&action=edit 12] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/13\_July\_2011&action=edit 13] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/14\_July\_2011&action=edit 14] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/15\_July\_2011&action=edit 15] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/16\_July\_2011&action=edit 16] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/17\_July\_2011&action=edit 17] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/18\_July\_2011&action=edit 18] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/19\_July\_2011&action=edit 19] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/20\_July\_2011&action=edit 20] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/21\_July\_2011&action=edit 21] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/22\_July\_2011&action=edit 22] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/23\_July\_2011&action=edit 23] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/24\_July\_2011&action=edit 24] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/25\_July\_2011&action=edit 25] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/26\_July\_2011&action=edit 26] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/27\_July\_2011&action=edit 27] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/28\_July\_2011&action=edit 28] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/29\_July\_2011&action=edit 29] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/30\_July\_2011&action=edit 30] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/31\_July\_2011&action=edit 31] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | August | | | | | | | | M | T | W | T | F | S | S | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/1\_August\_2011&action=edit 1] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/2\_August\_2011&action=edit 2] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/3\_August\_2011&action=edit 3] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/4\_August\_2011&action=edit 4] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/5\_August\_2011&action=edit 5] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/6\_August\_2011&action=edit 6] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/7\_August\_2011&action=edit 7] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/8\_August\_2011&action=edit 8] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/9\_August\_2011&action=edit 9] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/10\_August\_2011&action=edit 10] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/11\_August\_2011&action=edit 11] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/12\_August\_2011&action=edit 12] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/13\_August\_2011&action=edit 13] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/14\_August\_2011&action=edit 14] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/15\_August\_2011&action=edit 15] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/16\_August\_2011&action=edit 16] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/17\_August\_2011&action=edit 17] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/18\_August\_2011&action=edit 18] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/19\_August\_2011&action=edit 19] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/20\_August\_2011&action=edit 20] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/21\_August\_2011&action=edit 21] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/22\_August\_2011&action=edit 22] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/23\_August\_2011&action=edit 23] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/24\_August\_2011&action=edit 24] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/25\_August\_2011&action=edit 25] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/26\_August\_2011&action=edit 26] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/27\_August\_2011&action=edit 27] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/28\_August\_2011&action=edit 28] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/29\_August\_2011&action=edit 29] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/30\_August\_2011&action=edit 30] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/31\_August\_2011&action=edit 31] | |

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| |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | September | | | | | | | | M | T | W | T | F | S | S | |  |  |  | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/1\_September\_2011&action=edit 1] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/2\_September\_2011&action=edit 2] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/3\_September\_2011&action=edit 3] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/4\_September\_2011&action=edit 4] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/5\_September\_2011&action=edit 5] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/6\_September\_2011&action=edit 6] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/7\_September\_2011&action=edit 7] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/8\_September\_2011&action=edit 8] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/9\_September\_2011&action=edit 9] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/10\_September\_2011&action=edit 10] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/11\_September\_2011&action=edit 11] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/12\_September\_2011&action=edit 12] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/13\_September\_2011&action=edit 13] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/14\_September\_2011&action=edit 14] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/15\_September\_2011&action=edit 15] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/16\_September\_2011&action=edit 16] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/17\_September\_2011&action=edit 17] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/18\_September\_2011&action=edit 18] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/19\_September\_2011&action=edit 19] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/20\_September\_2011&action=edit 20] | [http://2011.igem.org/Team:UEA-JIC/Lab-entry/21\_September\_2011 21] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/22\_September\_2011&action=edit 22] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/23\_September\_2011&action=edit 23] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/24\_September\_2011&action=edit 24] | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/25\_September\_2011&action=edit 25] | | [http://2011.igem.org/wiki/index.php?title=Team:UEA-JIC/Lab-entry/26\_September\_2011&action=edit 26] | 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# STANDARD JOURNAL

**Thursday 23rd June** - Told last night after going to see X-men that we need to have a poster completed and ready for use by friday. Spent the day writing poster drafts and designing the layout, as well as attempting to copy the logo we designed onto the computer. We also updated the Wiki and gave it a new navigation bar, and looked into flight and hotel prices for Amsterdam!

**Friday 24th June** - Spent the day preparing for a brief presentation to the staff of the JIC so that we could prove we've actually done something! Researched more deeply into possible methods of ensuring light production only in the dark. We decided finally on three model organisms, Moss, Algae and E. coli, and divided the team into three sub-teams to work semi-independently on each project.

**Monday 27th June** - Today, we spent the morning researching moss and received our sample of algae in a nappy! A slideshow of photos was embedded onto the home page of the wiki and then we had lunch. After lunch we carried out transformations with 5 different biobricks, where the details can be viewed in the lab journal.

**Friday 1st July** - In the morning we ran a gel of our Miniprep of the BBA\_M30109 transformed E.coli cells. The Gel showed that we had indeed successfully isolated the plasmid from our cells, and so in the evening (LATE into the evening) we ran PCR protocol to try and amplify the specific gene we wanted from the plasmid (Cph8) We also continued to arrange the UK meet up, adding up numbers that were coming and trying to find speakers. We also added some finishing touches to the logo and placed it onto a business card for the team.

**Monday 4th july** - In the morning we ran a gel of our PCR products from Friday to test whether we had managed to amplify the specific gene we wanted. We also spent more time planning the UK iGEM get together, and decided on the protocol we would follow when transforming moss.

**Tuesday 5th july** - The gel from yesterday showed no DNA products from our PCR. We performed another PCR procedure using an adapted protocol and a different polymerase (phusion polymerase, as this has a proof reading ability and is therefore better suited for cloning). However, a gel from this was also unsuccessful. We returned to the drawing board and found a potential fault in the reverse primer we'd designed. Having corrected the error, we plan to order the adapted primer tomorrow. In light of specific numbers for the UK conference we began booking rooms and caterers. Having decided on a selection marker for algae transformations (arginine biosynthesis) we secured some arginine and autoclaved it ready for use tomorrow.

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