iGEM 2015 — Team UST_Beijing

# Team:UST Beijing/Notebook

[![](https://static.igem.org/mediawiki/2015/6/64/UST_Beijing_logo.png)](https://2015.igem.org/Team%3AUST_Beijing)

* [Home*![](https://static.igem.org/mediawiki/2015/8/87/UST_Beijing_home.png)*](https://2015.igem.org/Team%3AUST_Beijing)
* [Team*![](https://static.igem.org/mediawiki/2015/2/26/UST_Beijing_team.png)*](https://2015.igem.org/Team%3AUST_Beijing/Team)
* [Project*![](https://static.igem.org/mediawiki/2015/b/bc/UST_Beijing_project.png)*](https://2015.igem.org/Team%3AUST_Beijing/Project)
* [Parts*![](https://static.igem.org/mediawiki/2015/b/bc/UST_Beijing_parts.png)*](https://2015.igem.org/Team%3AUST_Beijing/Parts)
* [Modeling*![](https://static.igem.org/mediawiki/2015/8/80/UST_Beijing_modeling.png)*](https://2015.igem.org/Team%3AUST_Beijing/Modeling)
* [Outreach*![](https://static.igem.org/mediawiki/2015/1/12/UST_Beijing_outreach.png)*](https://2015.igem.org/Team%3AUST_Beijing/Outreach)
* [Notebook*![](https://static.igem.org/mediawiki/2015/0/0b/UST_Beijing_notebook.png)*](https://2015.igem.org/Team%3AUST_Beijing/Notebook)
* [Safety*![](https://static.igem.org/mediawiki/2015/a/aa/UST_Beijing_safety.png)*](https://2015.igem.org/Team%3AUST_Beijing/Safety)

##### 1.Petri dish

[![](https://static.igem.org/mediawiki/2015/e/e9/UST_Beijing_Notbook1.jpg)](https://static.igem.org/mediawiki/2015/e/e9/UST_Beijing_Notbook1.jpg)

E.coli which carries RFP plasmid grows into red colony.

##### 2.Extracting solution of RFP

Extract RFP from E.coli with TRITON.

[![](https://static.igem.org/mediawiki/2015/f/f8/UST_Beijing_Notbook2.jpg)](https://static.igem.org/mediawiki/2015/f/f8/UST_Beijing_Notbook2.jpg)

##### 3.Ultraviolet absorption curve

Using ultraviolet spectrophotometer to culture medium and to extract the red fluorescent protein to determine the absorbance curve.

[![](https://static.igem.org/mediawiki/2015/b/be/UST_Beijing_Notbook3.jpg)](https://static.igem.org/mediawiki/2015/b/be/UST_Beijing_Notbook3.jpg)

##### 4.Fluorescence microscope photographs

Different bacteria liquid medium, and sampling in different time, with dyed SYPR DNA escherichia coli individuals were observed under a fluorescence microscope in different time and red fluorescent protein expression under different culture conditions.

[![](https://static.igem.org/mediawiki/2015/c/c6/UST_Beijing_Notbook4.jpg)](https://static.igem.org/mediawiki/2015/c/c6/UST_Beijing_Notbook4.jpg)

##### 5.Optical filter

Of all sorts of color filter to determine the absorbance curve, finally choose the yellow filter with maximum light absorption

[![](https://static.igem.org/mediawiki/2015/c/c3/UST_Beijing_Notbook5.jpg)](https://static.igem.org/mediawiki/2015/c/c3/UST_Beijing_Notbook5.jpg)

##### 6.Sample container

Use our homemade quantitative measurement of red fluorescent protein LED by extraction of the filter device and yellow red fluorescent protein quantitatively determined.

[![](https://static.igem.org/mediawiki/2015/e/e7/UST_Beijing_Notbook6.jpg)](https://static.igem.org/mediawiki/2015/e/e7/UST_Beijing_Notbook6.jpg)

### Address

University of Science and Technology Beijing No.30, Xueyuan road, Haidian district, Beijing Province, China

Beijing City University No.269, Middle North 4th Ring Road, Haidian district, Beijing Province, China

![](https://static.igem.org/mediawiki/2015/6/64/UST_Beijing_logo.png)

Copyright © 2015.USTB&BCU All rights reserved.