iGEM 2018 — Team TU-Eindhoven

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# Team:TU-Eindhoven/Notebook

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* Project
  + [Design](https://2018.igem.org/Team%3ATU-Eindhoven/Design)
  + [Applied Design](https://2018.igem.org/Team%3ATU-Eindhoven/Applied_Design)
  + [Model](https://2018.igem.org/Team%3ATU-Eindhoven/Model)
  + [Demonstrate](https://2018.igem.org/Team%3ATU-Eindhoven/Demonstrate)
  + [Improve](https://2018.igem.org/Team%3ATU-Eindhoven/Improve)
  + [Safety](https://2018.igem.org/Team%3ATU-Eindhoven/Safety)
  + [Medal Requirements](https://2018.igem.org/Team%3ATU-Eindhoven/Medal_Requirements)
* Lab
  + [Notebook](https://2018.igem.org/Team%3ATU-Eindhoven/Notebook)
  + [Protocols](https://2018.igem.org/Team%3ATU-Eindhoven/Protocols)
  + [InterLab](https://2018.igem.org/Team%3ATU-Eindhoven/InterLab)
* Results
  + [Results Overview](https://2018.igem.org/Team%3ATU-Eindhoven/Results)
  + [Hydrogel](https://2018.igem.org/Team%3ATU-Eindhoven/Lab/Hydrogel)
  + [Adhesin](https://2018.igem.org/Team%3ATU-Eindhoven/Lab/Adhesin)
  + [Bacteriocin](https://2018.igem.org/Team%3ATU-Eindhoven/Lab/Bacteriocin)
* Parts
  + [Parts Overview](https://2018.igem.org/Team%3ATU-Eindhoven/Parts)
  + [Basic Parts](https://2018.igem.org/Team%3ATU-Eindhoven/Basic_Part)
  + [Composite Parts](https://2018.igem.org/Team%3ATU-Eindhoven/Composite_Part)
* Human Practices
  + [Human Practices](https://2018.igem.org/Team%3ATU-Eindhoven/Human_Practices)
  + [Education & Public Engagement](https://2018.igem.org/Team%3ATU-Eindhoven/Edu_n_Public_Engagement)
* Team
  + [Team Members](https://2018.igem.org/Team%3ATU-Eindhoven/Team)
  + [Collaborations](https://2018.igem.org/Team%3ATU-Eindhoven/Collaborations)
  + [Sponsors](https://2018.igem.org/Team%3ATU-Eindhoven/Sponsors)
  + [Attributions](https://2018.igem.org/Team%3ATU-Eindhoven/Attributions)

## Log book

Our logbook is recorded daily, here you can find a short summary of the labwork done by each subgroup in chronological order.

Categories:

(please select [ALL]:)
Adhesin
Cloning
Biobrick
Organic lab

[![](https://static.igem.org/mediawiki/2018/thumb/d/d5/T--TU-Eindhoven--Sponsor_ICSM.jpg/399px-T--TU-Eindhoven--Sponsor_ICSM.jpg)](https://www.tue.nl/en/research/research-labs/institute-for-complex-molecular-systems-laboratory/)
[![](https://static.igem.org/mediawiki/2018/1/10/T--TU-Eindhoven--Sponsor_PAMM.png)](http://www.pamm.nl/)
[![](https://static.igem.org/mediawiki/2018/1/1c/T--TU-Eindhoven--Sponsor_Hansaplast.png)](https://www.hansaplast.com/)
[![](https://static.igem.org/mediawiki/2018/thumb/6/63/T--TU-Eindhoven--Sponsor_IDT.png/320px-T--TU-Eindhoven--Sponsor_IDT.png)](https://eu.idtdna.com/pages)
[![](https://static.igem.org/mediawiki/2018/b/be/T--TU-Eindhoven--Sponsor_TUe.jpg)](https://www.tue.nl/)

![](https://static.igem.org/mediawiki/2018/4/47/T--TU-Eindhoven--mail.png) igem@tue.nl
[![](https://static.igem.org/mediawiki/2018/e/e7/T--TU-Eindhoven--facebook.png) iGEMTUEindhoven](https://www.facebook.com/iGEMTUEindhoven)


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:TU-Eindhoven/Experiments

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[![](https://static.igem.org/mediawiki/2018/thumb/1/16/T--TU-Eindhoven--LogoNavBar.png/320px-T--TU-Eindhoven--LogoNavBar.png)](https://2018.igem.org/Team%3ATU-Eindhoven)

* Project
  + [Design](https://2018.igem.org/Team%3ATU-Eindhoven/Design)
  + [Applied Design](https://2018.igem.org/Team%3ATU-Eindhoven/Applied_Design)
  + [Model](https://2018.igem.org/Team%3ATU-Eindhoven/Model)
  + [Demonstrate](https://2018.igem.org/Team%3ATU-Eindhoven/Demonstrate)
  + [Improve](https://2018.igem.org/Team%3ATU-Eindhoven/Improve)
  + [Safety](https://2018.igem.org/Team%3ATU-Eindhoven/Safety)
  + [Medal Requirements](https://2018.igem.org/Team%3ATU-Eindhoven/Medal_Requirements)
* Lab
  + [Notebook](https://2018.igem.org/Team%3ATU-Eindhoven/Notebook)
  + [Protocols](https://2018.igem.org/Team%3ATU-Eindhoven/Protocols)
  + [InterLab](https://2018.igem.org/Team%3ATU-Eindhoven/InterLab)
* Results
  + [Results Overview](https://2018.igem.org/Team%3ATU-Eindhoven/Results)
  + [Hydrogel](https://2018.igem.org/Team%3ATU-Eindhoven/Lab/Hydrogel)
  + [Adhesin](https://2018.igem.org/Team%3ATU-Eindhoven/Lab/Adhesin)
  + [Bacteriocin](https://2018.igem.org/Team%3ATU-Eindhoven/Lab/Bacteriocin)
* Parts
  + [Parts Overview](https://2018.igem.org/Team%3ATU-Eindhoven/Parts)
  + [Basic Parts](https://2018.igem.org/Team%3ATU-Eindhoven/Basic_Part)
  + [Composite Parts](https://2018.igem.org/Team%3ATU-Eindhoven/Composite_Part)
* Human Practices
  + [Human Practices](https://2018.igem.org/Team%3ATU-Eindhoven/Human_Practices)
  + [Education & Public Engagement](https://2018.igem.org/Team%3ATU-Eindhoven/Edu_n_Public_Engagement)
* Team
  + [Team Members](https://2018.igem.org/Team%3ATU-Eindhoven/Team)
  + [Collaborations](https://2018.igem.org/Team%3ATU-Eindhoven/Collaborations)
  + [Sponsors](https://2018.igem.org/Team%3ATU-Eindhoven/Sponsors)
  + [Attributions](https://2018.igem.org/Team%3ATU-Eindhoven/Attributions)

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# Experiments

* On this page no experiments are listed. Al the experiments performed in our project are documented on the [notebook](https://2018.igem.org/Team%3ATU-Eindhoven/Notebook), [protocols](https://2018.igem.org/Team%3ATU-Eindhoven/Protocols) and [results](https://2018.igem.org/Team%3ATU-Eindhoven/Results) pages.

[![](https://static.igem.org/mediawiki/2018/thumb/d/d5/T--TU-Eindhoven--Sponsor_ICSM.jpg/399px-T--TU-Eindhoven--Sponsor_ICSM.jpg)](https://www.tue.nl/en/research/research-labs/institute-for-complex-molecular-systems-laboratory/)
[![](https://static.igem.org/mediawiki/2018/1/10/T--TU-Eindhoven--Sponsor_PAMM.png)](http://www.pamm.nl/)
[![](https://static.igem.org/mediawiki/2018/1/1c/T--TU-Eindhoven--Sponsor_Hansaplast.png)](https://www.hansaplast.com/)
[![](https://static.igem.org/mediawiki/2018/thumb/6/63/T--TU-Eindhoven--Sponsor_IDT.png/320px-T--TU-Eindhoven--Sponsor_IDT.png)](https://eu.idtdna.com/pages)
[![](https://static.igem.org/mediawiki/2018/b/be/T--TU-Eindhoven--Sponsor_TUe.jpg)](https://www.tue.nl/)

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[![](https://static.igem.org/mediawiki/2018/e/e7/T--TU-Eindhoven--facebook.png) iGEMTUEindhoven](https://www.facebook.com/iGEMTUEindhoven)

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# Team:TU-Eindhoven/Results

Toggle navigation

[![](https://static.igem.org/mediawiki/2018/thumb/1/16/T--TU-Eindhoven--LogoNavBar.png/320px-T--TU-Eindhoven--LogoNavBar.png)](https://2018.igem.org/Team%3ATU-Eindhoven)

* Project
  + [Design](https://2018.igem.org/Team%3ATU-Eindhoven/Design)
  + [Applied Design](https://2018.igem.org/Team%3ATU-Eindhoven/Applied_Design)
  + [Model](https://2018.igem.org/Team%3ATU-Eindhoven/Model)
  + [Demonstrate](https://2018.igem.org/Team%3ATU-Eindhoven/Demonstrate)
  + [Improve](https://2018.igem.org/Team%3ATU-Eindhoven/Improve)
  + [Safety](https://2018.igem.org/Team%3ATU-Eindhoven/Safety)
  + [Medal Requirements](https://2018.igem.org/Team%3ATU-Eindhoven/Medal_Requirements)
* Lab
  + [Notebook](https://2018.igem.org/Team%3ATU-Eindhoven/Notebook)
  + [Protocols](https://2018.igem.org/Team%3ATU-Eindhoven/Protocols)
  + [InterLab](https://2018.igem.org/Team%3ATU-Eindhoven/InterLab)
* Results
  + [Results Overview](https://2018.igem.org/Team%3ATU-Eindhoven/Results)
  + [Hydrogel](https://2018.igem.org/Team%3ATU-Eindhoven/Lab/Hydrogel)
  + [Adhesin](https://2018.igem.org/Team%3ATU-Eindhoven/Lab/Adhesin)
  + [Bacteriocin](https://2018.igem.org/Team%3ATU-Eindhoven/Lab/Bacteriocin)
* Parts
  + [Parts Overview](https://2018.igem.org/Team%3ATU-Eindhoven/Parts)
  + [Basic Parts](https://2018.igem.org/Team%3ATU-Eindhoven/Basic_Part)
  + [Composite Parts](https://2018.igem.org/Team%3ATU-Eindhoven/Composite_Part)
* Human Practices
  + [Human Practices](https://2018.igem.org/Team%3ATU-Eindhoven/Human_Practices)
  + [Education & Public Engagement](https://2018.igem.org/Team%3ATU-Eindhoven/Edu_n_Public_Engagement)
* Team
  + [Team Members](https://2018.igem.org/Team%3ATU-Eindhoven/Team)
  + [Collaborations](https://2018.igem.org/Team%3ATU-Eindhoven/Collaborations)
  + [Sponsors](https://2018.igem.org/Team%3ATU-Eindhoven/Sponsors)
  + [Attributions](https://2018.igem.org/Team%3ATU-Eindhoven/Attributions)

# Overview of results

## [Hydrogel results](https://2018.igem.org/Team%3ATU-Eindhoven/Lab/Hydrogel)

We have successfully synthesized a bio-compatible gel with macroporous structure form pharmaceutical-grade dextran. This gel has been shown to be stable and free from toxic biproducts and contaminants. We produced several iterations of our hydrogels, culminating in a prototype with a central chamber.

## [Adhesin results](https://2018.igem.org/Team%3ATU-Eindhoven/Lab/Adhesin)

We have successfully transformed into *E. Coli* a modified gene for a sugar-binding adhesin originally discovered in arctic bacteria. We demonstrated this protein enabled bacteria to bind to the hydrogel to withstand vigorous washing. We also performed experiments with a prototype patch and demonstrated dramatic reduction in leakage of our adhesin-expressing bacteria compared to bacteria not expressing adhesin.

## [Modeling results](https://2018.igem.org/Team%3ATU-Eindhoven/Model)

We created 3 models to gain insights used to guide our design process.

Model 1 allowed us to predict the diffusivity of lysostaphin through a porous material,

Model 2 estimates the production of lysostaphin by *E. Coli*, and

Model 3 informed our design choices regarding patch dimensions and desired bacteria density and gave an idea about timescales during usage.

## [Bacteriocins](https://2018.igem.org/Team%3ATU-Eindhoven/Lab/Bacteriocin)

We have successfully expressed the bacteriocin lysostaphin in *E. Coli*  and have successfully fused it with a type I secretion tag, improving on an old biobrick originally registered by HIT Harbin 2012 . Through a series of experiments performed in collaboration with microbiology laboratory PAMM, we confirmed the production and the secretion of lysostaphin by living *E. Coli.*  Importantly, the experiments show that lysostaphin effectively kills *S. Aureus* and MRSA.

## [Biobricks](https://2018.igem.org/Team%3ATU-Eindhoven/Parts)

We added three composite parts and four basic parts. We have successfully improved a part made by a previous iGEM team and their project by demonstrating successful expression and secretion of lysostaphin.

During the 2018 Giant Jamboree, [BBa\_K2812004](http://parts.igem.org/Part%3ABBa_K2812004) was nominated by the jury for Best New Basic Part in the Overgrad competition. [BBa\_K2812005](http://parts.igem.org/Part%3ABBa_K2812005) was nominated by the jury for Best New Composite Part in the Overgrad competition.

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[![](https://static.igem.org/mediawiki/2018/thumb/d/d5/T--TU-Eindhoven--Sponsor_ICSM.jpg/399px-T--TU-Eindhoven--Sponsor_ICSM.jpg)](https://www.tue.nl/en/research/research-labs/institute-for-complex-molecular-systems-laboratory/)
[![](https://static.igem.org/mediawiki/2018/1/10/T--TU-Eindhoven--Sponsor_PAMM.png)](http://www.pamm.nl/)
[![](https://static.igem.org/mediawiki/2018/1/1c/T--TU-Eindhoven--Sponsor_Hansaplast.png)](https://www.hansaplast.com/)
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[![](https://static.igem.org/mediawiki/2018/b/be/T--TU-Eindhoven--Sponsor_TUe.jpg)](https://www.tue.nl/)

![](https://static.igem.org/mediawiki/2018/4/47/T--TU-Eindhoven--mail.png) igem@tue.nl
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