iGEM 2013 — Team ZJU-China

[![](/wiki/skins/common/images/wiki.jpg)"](/Main_Page "Main Page")

* [Page](/Team%3AZJU-China/Notebook/LabNotes/May)
* [Discussion](/wiki/index.php?title=Talk:Team:ZJU-China/Notebook/LabNotes/May&action=edit&redlink=1)
* [View source](/wiki/index.php?title=Team:ZJU-China/Notebook/LabNotes/May&action=edit)
* [History](/wiki/index.php?title=Team:ZJU-China/Notebook/LabNotes/May&action=history)
* teams

* [Log in](/wiki/index.php?title=Special:UserLogin&returnto=Team:ZJU-China/Notebook/LabNotes/May "You are encouraged to log in; however, it is not mandatory [o]")

# Team:ZJU-China/Notebook/LabNotes/May

### From 2013.igem.org

(Redirected from [Team:ZJU-China/Notebook](/wiki/index.php?title=Team:ZJU-China/Notebook&redirect=no "Team:ZJU-China/Notebook"))

### ZJU-China History

[2010: Bach](https://2010.igem.org/Team%3AZJU-China)
[2011: Rainbofilm](https://2011.igem.org/Team%3AZJU-China)
[2012: RiboScafford](https://2012.igem.org/Team%3AZJU-China)
[2013: Ghost Kit](https://2013.igem.org/Team%3AZJU-China)

### Wiki Menu

[![](https://static.igem.org/mediawiki/2013/c/cc/ZJU_logo_header.png)](https://2013.igem.org/Team%3AZJU-China "Home")

[![iGEM_logo](https://static.igem.org/mediawiki/2013/8/87/IGEMsmall_blue.png)](https://2013.igem.org/Main_Page "2013 iGEM Main Page")

* [Home](https://2013.igem.org/Team%3AZJU-China)
* [Project](https://2013.igem.org/Team%3AZJU-China/Project)
* [Team](https://2013.igem.org/Team%3AZJU-China/Team)
* [Appendix](https://2013.igem.org/Team%3AZJU-China/Notebook)
* [Human Practice](https://2013.igem.org/Team%3AZJU-China/HumanPractice)

* [![](https://static.igem.org/mediawiki/2013/8/8d/ZJU-header-home.JPG)

  Home
  Welcome to *E.coli*!](https://2013.igem.org/Team%3AZJU-China)
* [![](https://static.igem.org/mediawiki/2013/6/6f/ZJU-header-project.JPG)

  Project
  Ghost and Elf](https://2013.igem.org/Team%3AZJU-China/Project)

  [Bacterial Ghost](https://2013.igem.org/Team%3AZJU-China/Project/GhostSensor/Background)
  [The Ghost Kit](https://2013.igem.org/Team%3AZJU-China/Project/TheGhostKit/GhostSensor)
  [Characterization](https://2013.igem.org/Team%3AZJU-China/Project/Standardization/Background)
  [Safety](https://2013.igem.org/Team%3AZJU-China/Project/Safety/Overview)
  [Parts](http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&group=ZJU-China)
* [![](https://static.igem.org/mediawiki/2013/2/27/ZJU-header-team.JPG)

  Team
  Get to know us](https://2013.igem.org/Team%3AZJU-China/Team)

  [Meet Our Team](https://2013.igem.org/Team%3AZJU-China/Team/MeetOurTeam)
  [Attribution](https://2013.igem.org/Team%3AZJU-China/Team/Attribution)
  [Achievements](https://2013.igem.org/Team%3AZJU-China/Team/Achievements)
  [Acknowledgements](https://2013.igem.org/Team%3AZJU-China/Team/Acknowledge)
  [About ZJU](https://2013.igem.org/Team%3AZJU-China/Team/AboutOurUniversity)
* [![](https://static.igem.org/mediawiki/2013/d/d8/ZJU-header-notebook.JPG)

  Appendix
  We still have some more](https://2013.igem.org/Team%3AZJU-China/Notebook)

  [Lab Notes](https://2013.igem.org/Team%3AZJU-China/Notebook/LabNotes/May)
  [Protocol](https://2013.igem.org/Team%3AZJU-China/Notebook/Protocal)
  [Brainstorm](https://2013.igem.org/Team%3AZJU-China/Notebook/Brainstorm)
  [The "Elf"](https://2013.igem.org/Team%3AZJU-China/Notebook/TheElf/Background)
* [![](https://static.igem.org/mediawiki/2013/9/93/ZJU-header-humanpractice.JPG)

  Human Practice
  A real iGEM](https://2013.igem.org/Team%3AZJU-China/HumanPractice)

  [Overview](https://2013.igem.org/Team%3AZJU-China/HumanPractice/Overview)
  [The Tale](https://2013.igem.org/Team%3AZJU-China/HumanPractice/TheTale/Preface)
  [Synbio Chess Game](https://2013.igem.org/Team%3AZJU-China/HumanPractice/AChessGame)
  [Help Other Teams](https://2013.igem.org/Team%3AZJU-China/HumanPractice/BeyondTheTale/HelpOtherTeams)
  [Activities](https://2013.igem.org/Team%3AZJU-China/HumanPractice/BeyondTheTale/ScienceFair)

Lab Notes

[May](https://2013.igem.org/Team%3AZJU-China/Notebook/LabNotes/May)
[June](https://2013.igem.org/Team%3AZJU-China/Notebook/LabNotes/June)
[July](https://2013.igem.org/Team%3AZJU-China/Notebook/LabNotes/July)
[August](https://2013.igem.org/Team%3AZJU-China/Notebook/LabNotes/August)
[September](https://2013.igem.org/Team%3AZJU-China/Notebook/LabNotes/September)

Protocol

[Protocol](https://2013.igem.org/Team%3AZJU-China/Notebook/Protocal)

Brainstorm

[Brainstorm](https://2013.igem.org/Team%3AZJU-China/Notebook/Brainstorm)

The "Elf"

[Background](https://2013.igem.org/Team%3AZJU-China/Notebook/TheElf/Background)
[Design](https://2013.igem.org/Team%3AZJU-China/Notebook/TheElf/Design)
[Results](https://2013.igem.org/Team%3AZJU-China/Notebook/TheElf/Results)
[Perspetives](https://2013.igem.org/Team%3AZJU-China/Notebook/TheElf/Perspectives)

[![Flag Counter](http://s03.flagcounter.com/count/Nqab/bg_FFFFFF/txt_000000/border_CCCCCC/columns_2/maxflags_20/viewers_0/labels_1/pageviews_1/flags_0/)](http://info.flagcounter.com/Nqab)

## Lab Notes: May

| Date | Notes |
| --- | --- |
| May 24 | First lab day! Calibrate the pipettes, and check the working status of PCR machines. Made a plan for wet lab work. |
| May 25 | Set up new LB+Ch1 culture plates. BBa\_K411003 culture. Sterilization of culture plates, cuvettes, centrifuge tubes, and PCR tubes. |
| May 26 | PCR amplification of BBa\_K411003. |
| May 27 | Cut BBa\_K411003 and pSB1c3 with EcoR I and Pst I. |
| May 28 | Gel electrophoresis of the PCR products on May 27th. Failed because of inadequate operations. |
| May 29 | Collect the products of PCR on May 28th. Cut BBa\_K411003 with E+P and transform. |
| May 30 | Find out that pSB1c3 and pK411003 colonies are in good condition. Pick up colonies of these parts and transform again. |
| May 31 | Gel electrophoresis to analyze the results of transformation --- failed. Plasmid purification of pSB1c3. |

Previous: None

Next:
[Lab Notes: June](./June)

![Sponsors](https://static.igem.org/mediawiki/2013/3/37/ZJU_logo_college.png)

![The logo of Zhejiang University](https://static.igem.org/mediawiki/2013/4/45/ZJU-logo.png)

Retrieved from "[http://2013.igem.org/Team:ZJU-China/Notebook/LabNotes/May](http://2013.igem.org/Team%3AZJU-China/Notebook/LabNotes/May)"

[![Powered by MediaWiki](/wiki/skins/common/images/poweredby_mediawiki_88x31.png)](https://www.mediawiki.org/)

[![Attribution 3.0 Unported](https://i.creativecommons.org/l/by/3.0/88x31.png)](https://creativecommons.org/licenses/by/3.0/)

* [Recent changes](/Special%3ARecentChanges "Recent changes")
* [What links here](/Special%3AWhatLinksHere/Team%3AZJU-China/Notebook/LabNotes/May "List of all wiki pages that link here [j]")
* [Related changes](/Special%3ARecentChangesLinked/Team%3AZJU-China/Notebook/LabNotes/May "Recent changes in pages linked from this page [k]")
* [Special pages](/Special%3ASpecialPages "List of all special pages [q]")
* [My preferences](/Special%3APreferences)

* [Printable version](/wiki/index.php?title=Team:ZJU-China/Notebook/LabNotes/May&printable=yes "Printable version of this page [p]")
* [Permanent link](/wiki/index.php?title=Team:ZJU-China/Notebook/LabNotes/May&oldid=202724 "Permanent link to this revision of the page")
* [Privacy policy](/2013.igem.org%3APrivacy_policy "2013.igem.org:Privacy policy")
* [Disclaimers](/2013.igem.org%3AGeneral_disclaimer "2013.igem.org:General disclaimer")