iGEM 2017 — Team Pasteur_Paris

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# Team:Pasteur Paris/Notebook

[![](https://static.igem.org/mediawiki/2017/d/d8/T--Pasteur_Paris--aether.png)](https://2017.igem.org/Team%3APasteur_Paris)
[![](https://static.igem.org/mediawiki/2017/1/10/T--Pasteur_Paris--iGEM.png)](https://2017.igem.org/Team%3APasteur_Paris)

* Team
* Project
* Design
* Lab work
* Human Practice
* Achievements

[Our Team](https://2017.igem.org/Team%3APasteur_Paris/Team)

[Sponsors](https://2017.igem.org/Team%3APasteur_Paris/Team#pagesponsors)

[Attributions](https://2017.igem.org/Team%3APasteur_Paris/Attributions)

[Context](https://2017.igem.org/Team%3APasteur_Paris/Project)

[Entrepreneurship](https://2017.igem.org/Team%3APasteur_Paris/Entrepreneurship)

[Engagement](https://2017.igem.org/Team%3APasteur_Paris/Engagement)

[Model](https://2017.igem.org/Team%3APasteur_Paris/Model)

[Design Methodology](https://2017.igem.org/Team%3APasteur_Paris/Design)

[Applied
Design](https://2017.igem.org/Team%3APasteur_Paris/Applied_Design)

[Science](https://2017.igem.org/Team%3APasteur_Paris/Science)

[Notebook](https://2017.igem.org/Team%3APasteur_Paris/Notebook)

[Demonstrate](https://2017.igem.org/Team%3APasteur_Paris/Demonstrate)

[InterLab](https://2017.igem.org/Team%3APasteur_Paris/InterLab)

[Protocol](https://2017.igem.org/Team%3APasteur_Paris/Protocol)

[Safety
Security](https://2017.igem.org/Team%3APasteur_Paris/Safety)

[Law](https://2017.igem.org/Team%3APasteur_Paris/HP/Law)

[Collaborations](https://2017.igem.org/Team%3APasteur_Paris/HP/Collaborations)

[Human Practices](https://2017.igem.org/Team%3APasteur_Paris/HP/Silver)

[Integrated Practices](https://2017.igem.org/Team%3APasteur_Paris/HP/Gold_Integrated)

[Parts](https://2017.igem.org/Team%3APasteur_Paris/Parts)

[GiantJamboree](https://2017.igem.org/Team%3APasteur_Paris/GiantJamboree)

* [*photo\_camera*](https://www.instagram.com/igem.pasteur2017/)
* *email*

Labwork

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| July | | | | | | |
| M | T | W | T | F | S | S |
|

|  |  |  |  | 1 | 2 |

| 3 | 4 | 5 Operators: Deshmukh, Ersin, Gabriel, Alexis   Transformation of bacteria DH5α with pet43.1a.C162, for the positive control we will use the plasmid pUC19 + a negative control according to the transformation protocol except for volumes.      Observations 16h later:- • The culture for the positive test revealed mean bacterial cultures (more than 50) this test confirms our DH5α cells are competent - • The culture for the negative test did not reveal any bacterial culture, which proved that the cells without any plasmids couldn’t survive in an antibiotic based media - • The cell cultures containing pET43.1a.C162 do not present any colonies; there must be a problem with the pET43.1a.C162 solution, because the DH5α cells are truly competent - • By spectroscopy UV-visible using the equipment UV5 Nano Mettler Toledo, at 260 nm with a 1µL sample, we measured a plasmid concentration of 0, the solution used did not contain plasmids, which explains the death of our cultures   For the detailed protocols followed, refer to the PDF documents below | 6 Operators: Deshmukh, Ersin, Gabriel, Alexis   Transformation of plasmids pET43.1a.C162 in DH5α (2nd test) according to the transformation protocol except for volumes           For the detailed protocols followed, refer to the PDF documents below  Operators: Deshmukh, Ersin, Gabriel, Alexis   Petri Dish preparation according to the preparation protocol except for volumes. 33 Dish total    Then the Petri Dish are stored at 4°C or placed at the incubator at 37°C for immediate use.        For the detailed protocols followed, refer to the PDF documents below | 7 Culture in liquid media of DH5α containing pET43.1a.C162 according to the protocol of liquid culture except for the volumes.   - The pellet is stored at -20°C named: DH5α pET43.1a.C162.         For the detailed protocols followed, refer to the PDF documents below | 8 | 9 |
| 10 | 11 Operators: Deshmukh, Karima, Pierre-Louis, Juliette, Paul, Nathan, Diane   Culture in liquid media of DH5α containing pET43.1a.C162 according to the protocol of liquid culture except for the volumes.    Note: the Erlenmeyer number 5 was given 2 doses of antibiotics so we have 50µL of antibiotics for 25mL of LB broth media.          For the detailed protocols followed, refer to the PDF documents below | 12 Operators: Juliette, Karima, Paul   Midiprep of pET43.1a.C162 previous liquid culture with the Qiafilter Midi kit from Qiagen.    Four samples with an average concentration of 60 ng/µL where store at -20°C.  Operators: Ersin, Gabriel, Alexis   Digestion of the plasmid pET43.1a.C162 according to the digestion protocol except for the volumes with BamHI and XbaI.    Launch of an electrophoresis in order to extract the empty plasmid pET43.1a.    The cutted gel were stored at 4°C        For the detailed protocols followed, refer to the PDF documents below | 13 Operators: Ersin, Gabriel, Alexis   Gel extraction of the plasmid pET43.1a with the Qiagen gel extraction kit.    The sample is stored at -20°C, with an average concentration of 7,418 ng/µL.        For the detailed protocols followed, refer to the PDF documents below  Operators: Gabriel, Paul   Electrophoresis of pET43.1a.C162 midiprep from the 12/10/2017.    Check of the plasmid purity.        For the detailed protocols followed, refer to the PDF documents below | 14 | 15 | 16 |
| 17 Operators: Paul, Ersin, Diane, Alexis   Digestion of plasmids pET43.1a.C162    Launch of an electrophoresis in order to extract the empty plasmid pET43.1a.    The cutted gel were stored at 4°C        For the detailed protocols followed, refer to the PDF documents below | 18 Operators: Paul, Ersin, Gabriel, Diane   Gel extraction of the plasmid pET43.1a with the Qiagen gel extraction kit.    Four samples with an average concentration of 61 ng/µL where store at -20°C.        For the detailed protocols followed, refer to the PDF documents below | 19 Operators: Ersin, Alexis   Solubilisation of Eurofins Plasmid E2 gene in TE 0.1X : pEXA128-E2.     Operators: Diane, Ersin, Gabriel, Alexis, Karima    Protocol for Bacteria DH5α Transformation with pEXA128-E2         For the detailed protocols followed, refer to the PDF documents below  Operators: Gabriel, Ersin, Alexis, Diane, Karima   Dephosphorylation of plasmid or DNA: pET43.1a (X-B)        For the detailed protocols followed, refer to the PDF documents below | 20 | 21 | 22 | 23 Operator: Deshmukh   Liquid Culture for miniprep on transformed Bacteria DH5α pEXA128-E2.        For the detailed protocols followed, refer to the PDF documents below |
| 24 Operators: Alexis, Juliette   Petri Dish preparation according to the preparation protocol except for volumes.   20 Dish total    Then the Petri Dish are stored at 4°C or placed at the incubator at 37°C for immediate use.    Operator: Gabriel   Solubilisation of the lyophilized Eurofins plasmid pEXA128 E1\_2, for its future transformation    Operators: Alexis, Juliette, Karima   Transformation of DH5α with pSB1C3    Operators: Diane, Nathan   Transformation of DH5α with pEXA128 E1\_2.         For the detailed protocols followed, refer to the PDF documents below  Operators: Ersin, Gaétan   Miniprep of previous liquid culture with the QIAprep Spin Miniprep kit from Qiagen.   Four samples with an average concentration of 280 ng/µL.    Operators: Ersin, Gabriel   Digestion of the plasmid pEXA128 E2 according to the digestion protocol except for the volumes with BamHI and XbaI to retrieve our gene E2.    Operator: Gabriel   Electrophoresis gel preparation   Electrophoresis run        For the detailed protocols followed, refer to the PDF documents below | 25 Operators: Juliette Alexis    Liquid Culture for Miniprep on transformed Bacteria DH5α pEXA128-E1\_2    Operators: Karima, Diane    Gel Extraction of the gene E2 with QIAquick Gel Extraction Kit:  Operators: Karima, Juliette, Alexis    Transformation of DH5α by pSB1C3    Operators: Karima, Diane    Ligation between dephosphorylated pET43.1a and E2        For the detailed protocols followed, refer to the PDF documents below | 26 Operators: Karima, Diane    Miniprep on transformed Bacteria DH5α pEXA128-E1\_2    • Four samples with an average concentration of 320 ng/µL.    Plasmid Digestion and Gel extraction of pEXA128-E1\_2    Ligation of plasmid with DNA insert pEXA128-E1\_2        For the detailed protocols followed, refer to the PDF documents below  Operators: Ersin Gabriel    DNA Digestion pEXA128-E2        For the detailed protocols followed, refer to the PDF documents below | 27 | 28 | 29 | 30 |
| 31 Operators: Alexis, Ersin   Solubilisation of Eurofins Plasmid E3 gene: pEXA258-E3    Solubilisation of Eurofins Plasmid E1\_1 gene: pEXA258-E1\_1     Operators: Karima, Diane   DNA Digestion of pEX-E2 and pEX-E1\_2 with XbaI and BamHI        For the detailed protocols followed, refer to the PDF documents below  Operators: Alexis, Ersin   Transformation of DH5α with pEXA258-E1\_1:    Protocol for Bacteria Transformation of DH5α with pEXA258-E3        For the detailed protocols followed, refer to the PDF documents below |

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| August | | | | | | |
| M | T | W | T | F | S | S |
|

| 1 Operators: Diane, Ersin   Digestion of plasmid pET43.1a.C162    Electrophoresis    Gel Extraction with QIAquick Gel Extraction Kit        For the detailed protocols followed, refer to the PDF documents below  Operators: Diane, Alexis   Liquid Culture for Miniprep on transformed Bacteria DH5α pEXA258-E1\_1 and pEXA258-E3         For the detailed protocols followed, refer to the PDF documents below | 2 Operators: Ersin, Diane   Miniprep on transformed Bacteria DH5α pEXA258-E1\_1 and pEXA258-E3    Ligation of plasmid pET43.1a with DNA insert E2 col 2 and E1\_2 col 2    Gel Extraction of pET43.1a with QIAquick Gel Extraction Kit    Digestion of DNA pEXA258-E1\_1 and pEXA258-E3 with XbaI and BamHI         For the detailed protocols followed, refer to the PDF documents below  Operators: Ersin, Diane   Transformation of DH5α with pET43.1a-E1\_2    Transformation of DH5α with pET43.1a-E2 or pET43.1a    Dephosphorylation of plasmid pET43.1a with XbaI and BamHI        For the detailed protocols followed, refer to the PDF documents below | 3 Operators: Ersin, Diane   Gel Extraction of E1\_1 and E3 with QIAquick Gel Extraction Kit    Ligation of plasmid pET43.1a with DNA inserts E3 col 1 and E1-1 col 3        For the detailed protocols followed, refer to the PDF documents below  Operators: Diane, Alexis   Liquid Culture for Miniprep on transformed Bacteria DH5α pET43.1a-E2    Operators: Ersin, Diane   Protocol for Bacteria Transformation of DH5α with pET43.1a-E1-1 and pET43.1a-E3        For the detailed protocols followed, refer to the PDF documents below | 4 Operators: Alexis, Paul   Miniprep on transformed Bacteria DH5α pET43.1a-E2  Operators: Alexis, Paul   Digestion of pET43.1a-E2 with XbaI and BamHI        For the detailed protocols followed, refer to the PDF documents below | 5 | 6 |

| 7 Operators: Ersin, Diane   Digestion of pET43.1a-E1\_1 and pET43.1a-E1\_2 with XbaI and BamHI    DNA pET43.1a-E3 Digestion X-B        For the detailed protocols followed, refer to the PDF documents below  Operators: Ersin, Diane   Transformation BL21DE3 pET43.1a-E1\_1    Transformation BL21DE3 pET43.1a-E1\_2    Transformation BL21DE3 pET43.1a-E2    Transformation BL21DE3 pET43.1a-E3        For the detailed protocols followed, refer to the PDF documents below | 8 Operators: Gabriel, Diane   Preparation of solutions of plasmids pET43.1a-E1\_1, pET43.1a-E1\_2, pET43.1a-E2 and pET43.1a-E3, for sequencing by Eurofins    Operators: Diane, Azéline   Liquid Culture of transformed Bacteria BL21DE3 pET43.1a-E1-2 and pET43.1a-E2        For the detailed protocols followed, refer to the PDF documents below  Operators: Ersin, Diane   Transformation BL21DE3 pET43.1a-E1\_1    Transformation BL21DE3 pET43.1a-E1\_2    Transformation BL21DE3 pET43.1a-E2    Transformation BL21DE3 pET43.1a-E3         For the detailed protocols followed, refer to the PDF documents below | 9 Operators: Gaétan, Gabriel   Expression culture and kinetic BL21 pET43.1a-E1\_2 and pET43.1a-E2        For the detailed protocols followed, refer to the PDF documents below  Operators: Diane, Azéline   Transformation BL21DE3 pET43.1a-E1\_1, pET43.1a-E1\_2, pET43.1a-E2, pET43.1a-E3        For the detailed protocols followed, refer to the PDF documents below | 10 Operators: Gaétan, Gabriel   Liquid culture of BL21DE3 pET43.1a-E1-2 and pET43.1a-E2        For the detailed protocols followed, refer to the PDF documents below  Operators: Gabriel, Karima   Bacteria lysis and SDS Page gel migration of Bl21DE3 pET43.1a-E2 and pET43.1a-E1\_2         For the detailed protocols followed, refer to the PDF documents below | 11 Operators: Gaétan, Diane   Liquid culture of BL21De3 pET43.1a-E1-1 and pET43.1a-E3        For the detailed protocols followed, refer to the PDF documents below | 12 | 13 |
| 14 | 15 | 16 | 17 | 18 Operators: Gabriel, Diane, Alexis   Bacteria lysis and SDS Page gel migration        For the detailed protocols followed, refer to the PDF documents below | 19 | 20 |
| 21 Operators: Diane, Nathan   InterLab Transformation        For the detailed protocols followed, refer to the PDF documents below | 22 Operators : Ersin, Diane   Transformation BL21DE3 pET-E3    Transformation DH5α and BL21DE3 pET-E1\_1        For the detailed protocols followed, refer to the PDF documents below | 23 Operators: Alexis   Solubilisation of Eurofins Plasmid E4 gene: pEXA258-E4   Transformation DH5α pEX-E4         For the detailed protocols followed, refer to the PDF documents below | 24 | 25 | 26 | 27 |
| 28 Operators : Nathan, Diane   Transformation of DH5α with pSB1C3 of E1\_1, E1\_2, E2, E3 and E4        For the detailed protocols followed, refer to the PDF documents below | 29 Operators : Paul, Diane   Transformation of DH5α with pSB1C3 of E1\_1, E1\_2, E2, E3 and E4        For the detailed protocols followed, refer to the PDF documents below | 30 | 31 |

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| September | | | | | | |
| M | T | W | T | F | S | S |
|

|  |  |  | 1 | 2 | 3 |

| 4 | 5 | 6 Operators: Maud, Paul, Diane   DNA Digestion and Gel extraction of pET32a        For the detailed protocols followed, refer to the PDF documents below  Operators: Maud, Diane   Dephosphorylation of pET32a        For the detailed protocols followed, refer to the PDF documents below | 7 Operators : Maud, Diane   Transformation of DH5α with pET32a of E1\_1, E1\_2, E2, E3 and E4         For the detailed protocols followed, refer to the PDF documents below | 8 | 9 | 10 |
| 11 | 12 | 13 | 14 | 15 | 16 | 17 |
| 18 | 19 | 20 | 21 | 22 | 23 | 24 |
| 25 | 26 | 27 | 28 Operators: Ersin, Gaétan, Deshmukh   Miniprep on pEX-S1 transformed Bacteria        For the detailed protocols followed, refer to the PDF documents below  Operators: Ersin, Gaétan, Deshmukh   Transformation of DH5α with pSB1C3-E1\_1; E1\_2 and E2    Transformation of DH5α pET32a-E1\_1; E1\_2 and E2         For the detailed protocols followed, refer to the PDF documents below | 29 | 30 |

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| October | | | | | | |
| M | T | W | T | F | S | S |
|

|  |  |  |  |  | 1 |

| 2 | 3 | 4 | 5 Operators: Juliette and Ersin   Digestion of pET32a with E1\_1; E1\_2 and E2 | 6 | 7 Operators: Deshmukh and Juliette   Liquid culture of transformed bacteria and induction of protein expression        For the detailed protocols followed, refer to the PDF documents below  Operators: Juliette   Digestion if pSB1C3 by EcoRI and PstI   DNA Electrophoresis of pSB1C3 digested by EcoRI-PsTI        For the detailed protocols followed, refer to the PDF documents below | 8 Operators: Paul and Azéline   DNA Electrophoresis of S1 digested by EcoRI-PstI or XbaI-BamHI        For the detailed protocols followed, refer to the PDF documents below |
| 9 | 10 Operators: Juliette and Alexis   Gel Extraction with QIAquick Gel Extraction Kit of pSB1C3, S1 E-P and S1 X-B    Ligation of pSB1C3 with our parts E1\_1, E1\_2, E2, E3, E4, S1    DH5α Transformation with previous plasmid        For the detailed protocols followed, refer to the PDF documents below | 11 Operators: Juliette    Ligation of pET32a with S1    Transformation of DH5α with pET32a-S1         For the detailed protocols followed, refer to the PDF documents below | 12 | 13 | 14 | 15 |
| 16 | 17 | 18 | 19 Operators: Gabriel   Digestion of Biobricks made the 10-10-2017        For the detailed protocols followed, refer to the PDF documents below | 20 | 21 | 22 |
| 23 | 24 Operators: Paul   Liquid culture of DH5α with all of our biobricks for -80°C storage        For the detailed protocols followed, refer to the PDF documents below | 25 | 26 Operators : Juliette, Gabriel, Ersin    Reaction kinetics of the degradation of Anthracene    Please check our Demonstrate page for further information    For the detailed protocols followed, refer to the PDF documents below | 27 Operators : Juliette, Gabriel, Ersin    Reaction kinetics of the degradation of Anthracene    Please check our Demonstrate page for further information    For the detailed protocols followed, refer to the PDF documents below | 28 | 29 |
| 30 | 31 |

For blank protocols, please check our protocol page [here](https://2017.igem.org/Team%3APasteur_Paris/Protocol)

For the link between our Biobricks and the abreviations used in the protocols, please check our parts page [here](https://2017.igem.org/Team%3APasteur_Paris/Parts)

Week 1 protocols

[20170705](https://static.igem.org/mediawiki/2017/6/6a/T--Pasteur_Paris--20170705.pdf)
[20170706](https://static.igem.org/mediawiki/2017/c/c7/T--Pasteur_Paris--20170706.pdf)
[20170707](https://static.igem.org/mediawiki/2017/2/20/T--Pasteur_Paris--20170707.pdf)

Week 2 protocols

[20170711](https://static.igem.org/mediawiki/2017/3/35/T--Pasteur_Paris--20170711.pdf)
[20170712](https://static.igem.org/mediawiki/2017/e/e8/T--Pasteur_Paris--20170712.pdf)
[20170713](https://static.igem.org/mediawiki/2017/b/bd/T--Pasteur_Paris--20170713.pdf)

Week 3 protocols

[20170717](https://static.igem.org/mediawiki/2017/f/ff/T--Pasteur_Paris--20170717.pdf)
[20170718](https://static.igem.org/mediawiki/2017/d/db/T--Pasteur_Paris--20170718.pdf)
[20170719](https://static.igem.org/mediawiki/2017/5/5c/T--Pasteur_Paris--20170719.pdf)

Week 4 protocols

[20170723](https://static.igem.org/mediawiki/2017/8/8a/T--Pasteur_Paris--20170723.pdf)
[20170724](https://static.igem.org/mediawiki/2017/4/43/T--Pasteur_Paris--20170724.pdf)
[20170725](https://static.igem.org/mediawiki/2017/0/0e/T--Pasteur_Paris--20170725.pdf)
[20170726](https://static.igem.org/mediawiki/2017/c/c3/T--Pasteur_Paris--20170726.pdf)

Week 5 protocols

[20170731](https://static.igem.org/mediawiki/2017/4/47/T--Pasteur_Paris--20170731.pdf)
[20170801](https://static.igem.org/mediawiki/2017/3/38/T--Pasteur_Paris--20170801.pdf)
[20170802](https://static.igem.org/mediawiki/2017/e/e6/T--Pasteur_Paris--20170802.pdf)
[20170803](https://static.igem.org/mediawiki/2017/5/51/T--Pasteur_Paris--20170803.pdf)
[20170804](https://static.igem.org/mediawiki/2017/8/8d/T--Pasteur_Paris--20170804.pdf)

Week 6 protocols

[20170807](https://static.igem.org/mediawiki/2017/7/71/T--Pasteur_Paris--20170807.pdf)
[20170808](https://static.igem.org/mediawiki/2017/c/c8/T--Pasteur_Paris--20170808.pdf)
[20170809](https://static.igem.org/mediawiki/2017/f/f1/T--Pasteur_Paris--20170809.pdf)
[20170810](https://static.igem.org/mediawiki/2017/2/27/T--Pasteur_Paris--20170810.pdf)
[20170811](https://static.igem.org/mediawiki/2017/0/09/T--Pasteur_Paris--20170811.pdf)

Week 7 protocols

[20170818](https://static.igem.org/mediawiki/2017/c/cc/T--Pasteur_Paris--20170818.pdf)

Week 8 protocols

[20170821](https://static.igem.org/mediawiki/2017/b/b7/T--Pasteur_Paris--20170821.pdf)
[20170822](https://static.igem.org/mediawiki/2017/f/fc/T--Pasteur_Paris--20170822.pdf)
[20170823](https://static.igem.org/mediawiki/2017/b/b5/T--Pasteur_Paris--20170823.pdf)

Week 9 protocols

[20170829](https://static.igem.org/mediawiki/2017/1/1b/T--Pasteur_Paris--20170829.pdf)
[20170830](https://static.igem.org/mediawiki/2017/2/2d/T--Pasteur_Paris--20170830.pdf)

Week 10 protocols

[20170906](https://static.igem.org/mediawiki/2017/4/44/T--Pasteur_Paris--20170906.pdf)
[20170907](https://static.igem.org/mediawiki/2017/9/94/T--Pasteur_Paris--20170907.pdf)

Week 13 protocols

[20170928](https://static.igem.org/mediawiki/2017/8/8e/T--Pasteur_Paris--20170928.pdf)

Week 14 protocols

[20171005](https://static.igem.org/mediawiki/2017/0/07/T--Pasteur_Paris--20171005.pdf)
[20171007](https://static.igem.org/mediawiki/2017/4/42/T--Pasteur_Paris--20171007.pdf)
[20171008](https://static.igem.org/mediawiki/2017/7/71/T--Pasteur_Paris--20171008.pdf)

Week 15 protocols

[20171010](https://static.igem.org/mediawiki/2017/b/bb/T--Pasteur_Paris--20171010.pdf)
[20171011](https://static.igem.org/mediawiki/2017/f/f5/T--Pasteur_Paris--20171011.pdf)

Week 16 protocols

[20171019](https://static.igem.org/mediawiki/2017/4/49/T--Pasteur_Paris--20171019.pdf)

Week 17 protocols

[20171026](https://static.igem.org/mediawiki/2017/5/5b/T--Pasteur_Paris--20171026-20171027.pdf)
[20171027](https://static.igem.org/mediawiki/2017/5/5b/T--Pasteur_Paris--20171026-20171027.pdf)

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[![](https://static.igem.org/mediawiki/2017/7/71/T--Pasteur_Paris--genious_BW.png)](http://www.geneious.com)
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# Team:Pasteur Paris/Experiments

[![](http://placehold.it/350x150)](https://2017.igem.org/Team%3APasteur_Paris)

MENU

[HOME](https://2017.igem.org/Team%3APasteur_Paris)

TEAM

[Team](https://2017.igem.org/Team%3APasteur_Paris/Team)
[Collaborations](https://2017.igem.org/Team%3APasteur_Paris/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3APasteur_Paris/Description)
[Design](https://2017.igem.org/Team%3APasteur_Paris/Design)
[Experiments](https://2017.igem.org/Team%3APasteur_Paris/Experiments)
[Notebook](https://2017.igem.org/Team%3APasteur_Paris/Notebook)
[InterLab](https://2017.igem.org/Team%3APasteur_Paris/InterLab)
[Contribution](https://2017.igem.org/Team%3APasteur_Paris/Contribution)
[Model](https://2017.igem.org/Team%3APasteur_Paris/Model)
[Results](https://2017.igem.org/Team%3APasteur_Paris/Results)
[Demonstrate](https://2017.igem.org/Team%3APasteur_Paris/Demonstrate)
[Improve](https://2017.igem.org/Team%3APasteur_Paris/Improve)
[Attributions](https://2017.igem.org/Team%3APasteur_Paris/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3APasteur_Paris/Parts)
[Basic Parts](https://2017.igem.org/Team%3APasteur_Paris/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3APasteur_Paris/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3APasteur_Paris/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3APasteur_Paris/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3APasteur_Paris/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3APasteur_Paris/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3APasteur_Paris/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3APasteur_Paris/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3APasteur_Paris/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3APasteur_Paris/Hardware)
[Measurement](https://2017.igem.org/Team%3APasteur_Paris/Measurement)
[Model](https://2017.igem.org/Team%3APasteur_Paris/Model)
[Plant](https://2017.igem.org/Team%3APasteur_Paris/Plant)
[Software](https://2017.igem.org/Team%3APasteur_Paris/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=Pasteur_Paris)

# Pasteur\_Paris

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

##### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

##### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Pasteur Paris/Results

[![](http://placehold.it/350x150)](https://2017.igem.org/Team%3APasteur_Paris)

MENU

[HOME](https://2017.igem.org/Team%3APasteur_Paris)

TEAM

[Team](https://2017.igem.org/Team%3APasteur_Paris/Team)
[Collaborations](https://2017.igem.org/Team%3APasteur_Paris/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3APasteur_Paris/Description)
[Design](https://2017.igem.org/Team%3APasteur_Paris/Design)
[Experiments](https://2017.igem.org/Team%3APasteur_Paris/Experiments)
[Notebook](https://2017.igem.org/Team%3APasteur_Paris/Notebook)
[InterLab](https://2017.igem.org/Team%3APasteur_Paris/InterLab)
[Contribution](https://2017.igem.org/Team%3APasteur_Paris/Contribution)
[Model](https://2017.igem.org/Team%3APasteur_Paris/Model)
[Results](https://2017.igem.org/Team%3APasteur_Paris/Results)
[Demonstrate](https://2017.igem.org/Team%3APasteur_Paris/Demonstrate)
[Improve](https://2017.igem.org/Team%3APasteur_Paris/Improve)
[Attributions](https://2017.igem.org/Team%3APasteur_Paris/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3APasteur_Paris/Parts)
[Basic Parts](https://2017.igem.org/Team%3APasteur_Paris/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3APasteur_Paris/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3APasteur_Paris/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3APasteur_Paris/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3APasteur_Paris/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3APasteur_Paris/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3APasteur_Paris/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3APasteur_Paris/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3APasteur_Paris/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3APasteur_Paris/Hardware)
[Measurement](https://2017.igem.org/Team%3APasteur_Paris/Measurement)
[Model](https://2017.igem.org/Team%3APasteur_Paris/Model)
[Plant](https://2017.igem.org/Team%3APasteur_Paris/Plant)
[Software](https://2017.igem.org/Team%3APasteur_Paris/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=Pasteur_Paris)

# Pasteur\_Paris

# Results

Here you can describe the results of your project and your future plans.

##### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

##### You should also describe what your results mean:

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

##### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

##### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)