iGEM 2020 — Team SZ-SHD

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:SZ-SHD/Notebook

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:SZ-SHD/Engineering

# Engineering

Chitin is a stable natural polymer secreted by insects and many other organisms for protection and support. Such as the lining of insect pregut, which is plated with chitin to protect the soft tissue from harmful substances. It has been characterized that the presence of chitinase could degrade this chitin layer and reduce the growth rate of insects (i.e.: locusts) and eventually kill the individual. The lethality can be further enhanced with the aid of Bt toxins that disrupts the epithelial cells on intestines. Therefore, we introduce chitinase to our project, a type of enzyme that catalyst the hydrolysis of chitins to weakens and degrades this protective layer.

The first chitinase used is [ifchit](http://parts.igem.org/Part%3ABBa_K3686008), an endochitinase with molecular mass ~46kDa that originates from insect pathogenic fungi, Isaria fumosorosea. Which we have codon-optimized the gene, and transformed into E. coli BL21 for expression.

In order to characterize the production of ifchit, we assembled the plasmid pET28a-ifchit1 (pSS4) which allows the expression to be regulated under pTac system. Hence, we initiate protein synthesis by adding isopropyl β-D-1-thiogalactopyranoside(IPTG) when OD 600 ~0.6

![](https://static.igem.org/mediawiki/2020/thumb/5/56/T--SZ-SHD--EnginneringP1.png/800px-T--SZ-SHD--EnginneringP1.png)

[SZ-SHD](https://2020.igem.org/Team%3ASZ-SHD)

![](https://static.igem.org/mediawiki/2020/f/f0/T--SZ-SHD--BL.png)

![](https://static.igem.org/mediawiki/2020/1/19/T--SZ-SHD--SG.png)
![](https://static.igem.org/mediawiki/2020/f/fd/T--SZ-SHD--SZ.png)
![](https://static.igem.org/mediawiki/2020/a/ae/T--SZ-SHD--YJ.png)
![](https://static.igem.org/mediawiki/2020/4/42/T--SZ-SHD--DHSZ.png)

![](https://static.igem.org/mediawiki/2020/e/ec/T--SZ-SHD--aboutus.png)

![](https://static.igem.org/mediawiki/2020/7/75/T--SZ-SHD--theproject.png)

![](https://static.igem.org/mediawiki/2020/9/9a/T--SZ-SHD--parts.png)

![](https://static.igem.org/mediawiki/2020/d/d1/T--SZ-SHD--judging.png)

![](https://static.igem.org/mediawiki/2020/1/11/T--SZ-SHD--humanpractices.png)

![](https://static.igem.org/mediawiki/2020/a/a7/T--SZ-SHD--lab.png)

![](https://static.igem.org/mediawiki/2020/5/57/T--SZ-SHD--close.png)
![](https://static.igem.org/mediawiki/2020/8/8a/T--SZ-SHD--tree.png)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:SZ-SHD/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:SZ-SHD/Results

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)