iGEM 2019 — Team Tianjin

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# Team:Tianjin/Notebook

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* [Home](https://2019.igem.org/Team%3ATianjin)
* [Project](https://2019.igem.org/Team%3ATianjin/Description)
  + [Description](https://2019.igem.org/Team%3ATianjin/Description)
  + [Design](https://2019.igem.org/Team%3ATianjin/Design)
  + [Results](https://2019.igem.org/Team%3ATianjin/Demonstrate)
  + [Safety](https://2019.igem.org/Team%3ATianjin/Safety)
  + [Notebook](https://2019.igem.org/Team%3ATianjin/Notebook)
  + [Measurement](https://2019.igem.org/Team%3ATianjin/Measurement)
* [Parts](https://2019.igem.org/Team%3ATianjin/Parts)
  + [Parts Overview](https://2019.igem.org/Team%3ATianjin/Parts)
  + [Basic Part](https://2019.igem.org/Team%3ATianjin/Basic_Part)
  + [Composite Part](https://2019.igem.org/Team%3ATianjin/Composite_Part)
  + [Improved Part](https://2019.igem.org/Team%3ATianjin/Improved_Part)
* [Model](https://2019.igem.org/Team%3ATianjin/Model)
* [Human Practices](https://2019.igem.org/Team%3ATianjin/Human_Practices)
  + [Human Practices](https://2019.igem.org/Team%3ATianjin/Human_Practices)
  + [Integrated Practices](https://2019.igem.org/Team%3ATianjin/Integrated_Practices)
  + [Public Engagement](https://2019.igem.org/Team%3ATianjin/Public_Engagement)
  + [Collaborations](https://2019.igem.org/Team%3ATianjin/Collaborations)
* [Team](https://2019.igem.org/Team%3ATianjin/Team)
  + [Team](https://2019.igem.org/Team%3ATianjin/Team)
  + [Attributions](https://2019.igem.org/Team%3ATianjin/Attributions)

![](https://static.igem.org/mediawiki/2019/7/70/T--Tianjin--test2.png)

Notebook

## Protocol

## Notebook

## Reference

Sequence supportment: NCBI

We used the protocol of the paper below to fuse *S. cerevisiae* and *S.pombe*, and we have made some optimize.

[1]Gao Nianfa, Wang Shuhao, Li Xiaogang, Protoplast fusion between Saccharomyces cerevisiae and Schizosaccharomyces cerevisiae to isolate wine yeast with strong malic acid degradation[J].Chinese Journal of Biotechnology,2000,16(6):1- 5.

We used the protocol of the paper below to fuse *S. cerevisiae* and *Y. lipolytica* and we have made some optimize.

[2]Sipiczki M , Ferenczy L . Protoplast fusion of Schizosaccharomyces pombe Auxotrophic mutants of identical mating-type[J]. MGG - Molecular and General Genetics, 1977, 151(1):77-81.

We extracted the method of *S. cerevisiae* protoplast preparation and optimized and rectified it.

[3]Zhang Borun, Wang Yonghong, Liu Shufeng, et al. Fusion of Saccharomyces cerevisiae protoplasts[J]. Bulletin of Microbiology, 1986(2

We extracted the method for preparing *Y. lipolytica* protoplasts and we make the optimization of the protocol.

[4]Hou Xiaojiao, Xu Kun. Preparation and regeneration conditions of protoplasts of Yarrowia lipolytica[J]. Journal of Liaodong University (Natural Science Edition), 2019, 26(01): 47-53.

#### About us

* Team Tianjin 2019 is an undergraduate team from the Institute of Chemical Engineering, Tianjin University. This year, our project aims to develop a chromosome-based gene transfer technology between different cells by designing chromosome stabilization element group, using cell fusion as a means to characterize the effect of chromosome transfer. We also designed a series of detection methods and a single base editing system based on CRISPR technology.

#### Contact information

* Wechat: iGEM 天津
* Email: igem.tianjin@gmail.com
* Address: School of Chemical Engineering, Tianjin University, 135 Yaguan Road, Jinnan District, Tianjin


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# Team:Tianjin/Experiments

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[HOME](https://2019.igem.org/Team%3ATianjin)

TEAM

[Team Members](https://2019.igem.org/Team%3ATianjin/Team)
[Collaborations](https://2019.igem.org/Team%3ATianjin/Collaborations)

PROJECT

[Description](https://2019.igem.org/Team%3ATianjin/Description)
[Design](https://2019.igem.org/Team%3ATianjin/Design)
[Experiments](https://2019.igem.org/Team%3ATianjin/Experiments)
[Notebook](https://2019.igem.org/Team%3ATianjin/Notebook)
[Contribution](https://2019.igem.org/Team%3ATianjin/Contribution)
[Results](https://2019.igem.org/Team%3ATianjin/Results)
[Demonstrate](https://2019.igem.org/Team%3ATianjin/Demonstrate)
[Improve](https://2019.igem.org/Team%3ATianjin/Improve)
[Attributions](https://2019.igem.org/Team%3ATianjin/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3ATianjin/Parts)
[Basic Parts](https://2019.igem.org/Team%3ATianjin/Basic_Part)
[Composite Parts](https://2019.igem.org/Team%3ATianjin/Composite_Part)
[Part Collection](https://2019.igem.org/Team%3ATianjin/Part_Collection)

[SAFETY](https://2019.igem.org/Team%3ATianjin/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3ATianjin/Human_Practices)
[Education & Engagement](https://2019.igem.org/Team%3ATianjin/Public_Engagement)

AWARDS

[Entrepreneurship](https://2019.igem.org/Team%3ATianjin/Entrepreneurship)
[Hardware](https://2019.igem.org/Team%3ATianjin/Hardware)
[Measurement](https://2019.igem.org/Team%3ATianjin/Measurement)
[Model](https://2019.igem.org/Team%3ATianjin/Model)
[Plant](https://2019.igem.org/Team%3ATianjin/Plant)
[Software](https://2019.igem.org/Team%3ATianjin/Software)

[JUDGING FORM ⇗](https://igem.org/2019_Judging_Form?team=Tianjin)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

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# Team:Tianjin/Results

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[HOME](https://2019.igem.org/Team%3ATianjin)

TEAM

[Team Members](https://2019.igem.org/Team%3ATianjin/Team)
[Collaborations](https://2019.igem.org/Team%3ATianjin/Collaborations)

PROJECT

[Description](https://2019.igem.org/Team%3ATianjin/Description)
[Design](https://2019.igem.org/Team%3ATianjin/Design)
[Experiments](https://2019.igem.org/Team%3ATianjin/Experiments)
[Notebook](https://2019.igem.org/Team%3ATianjin/Notebook)
[Contribution](https://2019.igem.org/Team%3ATianjin/Contribution)
[Results](https://2019.igem.org/Team%3ATianjin/Results)
[Demonstrate](https://2019.igem.org/Team%3ATianjin/Demonstrate)
[Improve](https://2019.igem.org/Team%3ATianjin/Improve)
[Attributions](https://2019.igem.org/Team%3ATianjin/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3ATianjin/Parts)
[Basic Parts](https://2019.igem.org/Team%3ATianjin/Basic_Part)
[Composite Parts](https://2019.igem.org/Team%3ATianjin/Composite_Part)
[Part Collection](https://2019.igem.org/Team%3ATianjin/Part_Collection)

[SAFETY](https://2019.igem.org/Team%3ATianjin/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3ATianjin/Human_Practices)
[Education & Engagement](https://2019.igem.org/Team%3ATianjin/Public_Engagement)

AWARDS

[Entrepreneurship](https://2019.igem.org/Team%3ATianjin/Entrepreneurship)
[Hardware](https://2019.igem.org/Team%3ATianjin/Hardware)
[Measurement](https://2019.igem.org/Team%3ATianjin/Measurement)
[Model](https://2019.igem.org/Team%3ATianjin/Model)
[Plant](https://2019.igem.org/Team%3ATianjin/Plant)
[Software](https://2019.igem.org/Team%3ATianjin/Software)

[JUDGING FORM ⇗](https://igem.org/2019_Judging_Form?team=Tianjin)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the part's Main Page on the Registry.** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)