iGEM 2019 — Team CSL_Pittsburgh

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# Team:CSL Pittsburgh/Notebook

Home

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* [Home(current)](https://2019.igem.org/Team%3ACSL_Pittsburgh)
* [Team](https://2019.igem.org/Team%3ACSL_Pittsburgh/Team)
* [Project](https://2019.igem.org/Team%3ACSL_Pittsburgh/Description)
* [Parts](https://2019.igem.org/Team%3ACSL_Pittsburgh/Parts)
* [Notebook](https://2019.igem.org/Team%3ACSL_Pittsburgh/Notebook)
* [Attributions](https://2019.igem.org/Team%3ACSL_Pittsburgh/Attributions)
* [Collaborations](https://2019.igem.org/Team%3ACSL_Pittsburgh/Collaborations)
* [Human Practices](https://2019.igem.org/Team%3ACSL_Pittsburgh/Human_Practices)

## **Notebook**

### Protocols

* Part BBa\_K112624 in psb1c3 was transformed into DH5 competent cells using pitts protocol called piGEM plasmids.

* Colony pcr of piGEM was performed to make sure insert was present.

* Digestion of IDT oligos and linearized psb1c3 plasmid was done and ligated overnight. It was
  transformed with pitts transformation protocol.

* Colony PCR was performed for the pCYP6AY1 was performed to make sure insert was present.

* pCYP6AY1 and piGEM were plasmid prepped to obtain purified plasmid. IBI kit.

* Plasmids were digested with BamHI and NdeI. No band was observed for piGEM that we were going to insert into pCYP6AY1.

* Single digests of piGEM were done with BamHI and NdeI to see if they digested. BamHI did not digest the plasmid.

* Returned to our transformants from step 3 for the other oligo inserted into pSB1C3. Resulted in pBee plasmid.

* Colony PCR on pBee colony transformants to see if any contained insert.

* Used the positive culture EpBee and WT dh5 alpha in imidacloprid to see if there was a significant difference in their growth.

* Used WT and EpBee cultures to extract his tagged proteins in the supernatant and quantified with nanodrop.

* Fed the WT and EpBee cultures to bees and tested their survival with imidacloprid treatment.

![](https://static.igem.org/mediawiki/2019/3/32/T--CSL_Pittsburgh--AlexisBees.jpeg)


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# Team:CSL Pittsburgh/Experiments

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[HOME](https://2019.igem.org/Team%3ACSL_Pittsburgh)

TEAM

[Team Members](https://2019.igem.org/Team%3ACSL_Pittsburgh/Team)
[Collaborations](https://2019.igem.org/Team%3ACSL_Pittsburgh/Collaborations)

PROJECT

[Description](https://2019.igem.org/Team%3ACSL_Pittsburgh/Description)
[Design](https://2019.igem.org/Team%3ACSL_Pittsburgh/Design)
[Experiments](https://2019.igem.org/Team%3ACSL_Pittsburgh/Experiments)
[Notebook](https://2019.igem.org/Team%3ACSL_Pittsburgh/Notebook)
[Contribution](https://2019.igem.org/Team%3ACSL_Pittsburgh/Contribution)
[Results](https://2019.igem.org/Team%3ACSL_Pittsburgh/Results)
[Demonstrate](https://2019.igem.org/Team%3ACSL_Pittsburgh/Demonstrate)
[Improve](https://2019.igem.org/Team%3ACSL_Pittsburgh/Improve)
[Attributions](https://2019.igem.org/Team%3ACSL_Pittsburgh/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3ACSL_Pittsburgh/Parts)
[Basic Parts](https://2019.igem.org/Team%3ACSL_Pittsburgh/Basic_Part)
[Composite Parts](https://2019.igem.org/Team%3ACSL_Pittsburgh/Composite_Part)
[Part Collection](https://2019.igem.org/Team%3ACSL_Pittsburgh/Part_Collection)

[SAFETY](https://2019.igem.org/Team%3ACSL_Pittsburgh/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3ACSL_Pittsburgh/Human_Practices)
[Education & Engagement](https://2019.igem.org/Team%3ACSL_Pittsburgh/Public_Engagement)

AWARDS

[Entrepreneurship](https://2019.igem.org/Team%3ACSL_Pittsburgh/Entrepreneurship)
[Hardware](https://2019.igem.org/Team%3ACSL_Pittsburgh/Hardware)
[Measurement](https://2019.igem.org/Team%3ACSL_Pittsburgh/Measurement)
[Model](https://2019.igem.org/Team%3ACSL_Pittsburgh/Model)
[Plant](https://2019.igem.org/Team%3ACSL_Pittsburgh/Plant)
[Software](https://2019.igem.org/Team%3ACSL_Pittsburgh/Software)

[JUDGING FORM ⇗](https://igem.org/2019_Judging_Form?team=CSL_Pittsburgh)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:CSL Pittsburgh/Results

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[HOME](https://2019.igem.org/Team%3ACSL_Pittsburgh)

TEAM

[Team Members](https://2019.igem.org/Team%3ACSL_Pittsburgh/Team)
[Collaborations](https://2019.igem.org/Team%3ACSL_Pittsburgh/Collaborations)

PROJECT

[Description](https://2019.igem.org/Team%3ACSL_Pittsburgh/Description)
[Design](https://2019.igem.org/Team%3ACSL_Pittsburgh/Design)
[Experiments](https://2019.igem.org/Team%3ACSL_Pittsburgh/Experiments)
[Notebook](https://2019.igem.org/Team%3ACSL_Pittsburgh/Notebook)
[Contribution](https://2019.igem.org/Team%3ACSL_Pittsburgh/Contribution)
[Results](https://2019.igem.org/Team%3ACSL_Pittsburgh/Results)
[Demonstrate](https://2019.igem.org/Team%3ACSL_Pittsburgh/Demonstrate)
[Improve](https://2019.igem.org/Team%3ACSL_Pittsburgh/Improve)
[Attributions](https://2019.igem.org/Team%3ACSL_Pittsburgh/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3ACSL_Pittsburgh/Parts)
[Basic Parts](https://2019.igem.org/Team%3ACSL_Pittsburgh/Basic_Part)
[Composite Parts](https://2019.igem.org/Team%3ACSL_Pittsburgh/Composite_Part)
[Part Collection](https://2019.igem.org/Team%3ACSL_Pittsburgh/Part_Collection)

[SAFETY](https://2019.igem.org/Team%3ACSL_Pittsburgh/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3ACSL_Pittsburgh/Human_Practices)
[Education & Engagement](https://2019.igem.org/Team%3ACSL_Pittsburgh/Public_Engagement)

AWARDS

[Entrepreneurship](https://2019.igem.org/Team%3ACSL_Pittsburgh/Entrepreneurship)
[Hardware](https://2019.igem.org/Team%3ACSL_Pittsburgh/Hardware)
[Measurement](https://2019.igem.org/Team%3ACSL_Pittsburgh/Measurement)
[Model](https://2019.igem.org/Team%3ACSL_Pittsburgh/Model)
[Plant](https://2019.igem.org/Team%3ACSL_Pittsburgh/Plant)
[Software](https://2019.igem.org/Team%3ACSL_Pittsburgh/Software)

[JUDGING FORM ⇗](https://igem.org/2019_Judging_Form?team=CSL_Pittsburgh)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the part's Main Page on the Registry.** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)