iGEM 2009 — Team UC_Davis

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# Team:UC Davis/Notebook

### From 2009.igem.org

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**[![](https://static.igem.org/mediawiki/2009/a/a4/UCDAVIS_PIC3.png)](https://2009.igem.org/Team%3AUC_Davis)** [**![](https://static.igem.org/mediawiki/2009/d/d1/UCDAVIS_PIC4.png)**](https://2009.igem.org/Team%3AUC_Davis/About_Us)**[![](https://static.igem.org/mediawiki/2009/b/b9/UCDAVIS_PIC8.png)](https://2009.igem.org/Team%3AUC_Davis/Project)** **[![](https://static.igem.org/mediawiki/2009/2/2f/UCDAVIS_PIC5.png)](https://2009.igem.org/Team%3AUC_Davis/Notebook)** **[![](https://static.igem.org/mediawiki/2009/a/a6/UCDAVIS_PIC6.png)](https://2009.igem.org/Team%3AUC_Davis/Parts) [![](https://static.igem.org/mediawiki/2009/1/1d/UCDAVIS_PIC7.png)](https://2009.igem.org/Team%3AUC_Davis/Contacts_References)**

---

**[July](#july)
       [August](#august)
             [September](#september)
[Results](#Results)

![](https://static.igem.org/mediawiki/2009/5/58/UCDAVIS_picture2.jpg)**

---

July:

Before beginning the wet lab
portion of our project, we have read numerous articles and journals
relevant to our proposed project, and have utilized this information to
guide our part designs.  After significant research, design and
redesign, we have settled on the final design for our two key parts,
and plan for their construction.

    We
have begun ordering the necessary reagents for our work.  This
includes, selecting the parts required for our project from those
provided by the iGEM parts distribution, oligonucleotides for
extracting them, and for the synthesis of novel parts via Polymerase
Chain Reaction, PCR, and the DNA synthesis of several novel parts from
GENEART.
        We have recently started the
synthesis and assembly of the initial elements required for the
secretion system.

---

August:
        After reading the numerous
articles we dug up in regards to our project, we have designed our
project; in blue print form of structural wet lab experimentation, as
well as the techniques, materials, equipment, and time required to
complete our project.

        The parts and a majority of the
reagents we have ordered necessary for our work have arrived, and we
are in the stages of conducting experiments to construct our
parts.  We have to construct, test, and prove through sequencing
as well as other miscellaneous techniques that we truly have the parts
we want in our constructs.  So the progress for the month of
August varies greatly on almost a daily basis.

Apart from the wet lab portion of our project, we
have also begun the initial stages of building our wiki.  We have
begun to revise our information regarding the team, biomedical
engineering, and how it is UC Davis’s inauguration year into the iGEM
competition.

---

September:

    We are putting together the final pieces
of our intended construct and
have designed the tiny details of our experiments’ proper controls.The
design itself is useless without making it as a physical part, so we
are already building these simultaneously with our experimental
constructs.
    The wiki
is now up on the iGEM website and is being reviewed countless times to
ensure accuracy and appeal.
    We have also designed team t-shirts, which are a
tradition of iGEM, and are currently in the process of making them into
print.We are
also making the presentation power point for presentation at the
competition. We are
also designed the poster for presentation at the competition.

---

Results:

Data collection strategy:

Strain:
1. (no induction) ------> Spin cells to seperate cells from media
------> Check for fluorescence or western blot with 6-His Tag
2. (induced with IPTG) ------> Spin cells to seperate
cells from media ------> Check for fluorescence or
western blot with 6-His Tag

![](https://static.igem.org/mediawiki/2009/6/65/UCDAVIS_Exp_gel1.png)

Whole cells were lyced and ran on this gel. The difference in protein
concentrations are shown between the different plasmid containing
cells. Each well was loaded with a cell containing the vectors labeled
on top of the well.

INPNC\_SS\_GFP
whole cell fluorescence spectrum

![](https://static.igem.org/mediawiki/2009/7/73/UCDAVIS_Graph1.jpg)
Ex: 395 nm
Green = INPNC.SS.GFP induced
red = INPNC.SS.GFP uninduced

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