iGEM 2014 — Team TU_Eindhoven

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# Team:TU Eindhoven/Notebook

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* [Home](https://2014.igem.org/Team%3ATU_Eindhoven)
* Team ▼
  + [Team Members](https://2014.igem.org/Team%3ATU_Eindhoven/Team)
  + [Attributions](https://2014.igem.org/Team%3ATU_Eindhoven/Attributions)
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  + [Acknowledgements](https://2014.igem.org/Team%3ATU_Eindhoven/Acknowledgements)
* Project ▼
  + [Abstract](https://2014.igem.org/Team%3ATU_Eindhoven/Abstract)
  + [Overview](https://2014.igem.org/Team%3ATU_Eindhoven/Overview)
  + [Project Description](https://2014.igem.org/Team%3ATU_Eindhoven/Project)
  + [Background ►](https://2014.igem.org/Team%3ATU_Eindhoven/Background)
    - [SPAAC Reaction](https://2014.igem.org/Team%3ATU_Eindhoven/SPAAC)
    - [Anchors](https://2014.igem.org/Team%3ATU_Eindhoven/Background_Anchors)
    - [FACS](https://2014.igem.org/Team%3ATU_Eindhoven/FACS)
  + [Design ►](https://2014.igem.org/Team%3ATU_Eindhoven/Design)
    - [Membrane Anchors](https://2014.igem.org/Team%3ATU_Eindhoven/Design_Anchors)
    - [Plasmid Design](https://2014.igem.org/Team%3ATU_Eindhoven/Plamid_Design)
  + [Click Reaction ►](https://2014.igem.org/Team%3ATU_Eindhoven/Click_Reaction)
  + RCA ►
    - [Introduction](https://2014.igem.org/Team%3ATU_Eindhoven/Introduction)
    - [Mechanism](https://2014.igem.org/Team%3ATU_Eindhoven/Mechanism)
    - [Results](https://2014.igem.org/Team%3ATU_Eindhoven/Plamid_Design)
  + [ZAP](https://2014.igem.org/Team%3ATU_Eindhoven/ZAP)
  + [Main Results](https://2014.igem.org/Team%3ATU_Eindhoven/Project/Main_results)
  + [Submitted Parts](https://2014.igem.org/Team%3ATU_Eindhoven/Project/Parts)
  + [Medal Motivation](https://2014.igem.org/Team%3ATU_Eindhoven/Project/Medal_motivation)
  + [Future Perspectives](https://2014.igem.org/Team%3ATU_Eindhoven/Future_Perspectives)
* [Microfluidics ▼](https://2014.igem.org/Team%3ATU_Eindhoven/Project/Microfluidics)
  + [Introduction](https://2014.igem.org/Team%3ATU_Eindhoven/Project/Microfluidics_introduction)
  + [Droplet Device](https://2014.igem.org/Team%3ATU_Eindhoven/Project/Microfluidics_Dropletdevice)
  + [Cell Encapsulation](https://2014.igem.org/Team%3ATU_Eindhoven/Project/Microfluidics_cellencapsulation)
  + [Results](https://2014.igem.org/Team%3ATU_Eindhoven/Project/Microfluidics_results)
* [Modeling](https://2014.igem.org/Team%3ATU_Eindhoven/Project/Modeling)
* [Notebook ▼](https://2014.igem.org/Team%3ATU_Eindhoven/Notebook)
  + [Timeline](https://2014.igem.org/Team%3ATU_Eindhoven/Project/protocols)
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  + [Logbook](https://2014.igem.org/Team%3ATU_Eindhoven/Project/protocols)
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  + [Policy & Practices](https://2014.igem.org/Team%3ATU_Eindhoven/Project/Policyandpractices)
'* [Safety ▼](https://2014.igem.org/Team%3ATU_Eindhoven/Safety)
  + [Our Lab](https://2014.igem.org/Team%3ATU_Eindhoven/Project/Our_lab)
  + [Biosafety](https://2014.igem.org/Team%3ATU_Eindhoven/Project/Biosafety)
* [Sponsors](https://2014.igem.org/Team%3ATU_Eindhoven/Sponsors)
* [![](https://static.igem.org/mediawiki/2014/0/0e/TU_Eindhoven_iGEM.png)](https://2014.igem.org)

TU Eindhoven iGEM Team

## Notebook

* [JANUARY](#january)
* [FEBRUARY](#february)
* [MARCH](#march)
* [APRIL](#april)
* [MAY](#may)
* [JUNE](#june)
* [JULY](#july)
* [AUGUST](#august)
* [SEPTEMBER](#september)
* [OCTOBER](#october)

# January

-Background research

# February

-Background research

# March

-Selection of topic
-Further research
-Selection of projec

# April

-Further research

# May

**General Activities**
Regular team meetings
Looking for sponsors
Month planning

# June

**Wetlab**
- Arrival of the biobricks
- The designs for the plasmids are approved and ordered Primers
- Preparation of culture media, agar plates, antibiotics and glycerol
- Amplification of PET-29a(+), pEVOL-pAzF, BBa\_K811005(Penn) & CPX

# July

**Wetlab**
**Week 27: 30 June – 6 July**
Plasmid amplification and culturing BBa\_K811005(Penn) + CPX
Vector and insert Digestions
BBa\_K811005(Penn) + CPX insert ligate pET29a
Colony PCR

**Week 28: 7 July – 13 July**
Colony PCR
Site directed mutagenesis of CPX

**Week 29: 14 July – 20 July**
Site directed mutagenesis of CPX
Sequencing of CPX and BBa\_K811005(Penn)

**Week 30: 21 July – 27 July**
Sequencing results came in and were as expected
Protein expression
FACS – DBCO-PEG4-5/6-TAMRA

**Week 31: 28 July – August 3**
Protein expression
FACS – DBCO-PEG10kDa

# August

**Wetlab**
**Week 32: 4 August – 10 August**
Protein expression
FACS – Antibody titration
Protein expression curve

**Week 33: 11 August – 17 August**
FACS - DBCO PEG-5kDa fluorescent

**Week 34: 18 August – 24 August**
Start Bio-bricking
Start silencing mutagenesis

**Week 35: 25 August – 31 August**

# September

**Wetlab**
**Week 36: 1 September – 7 September**

**Week 37: 8 September – 14 September**

**Week 38: 15 September – 21 September**

**Week 39: 22 September – 28 September**

# October

**Wetlab**
**Week 40: 29 September – 5 October**

**Week 41: 6 October – 12 October**

**Week 42: 13 October – 19 October**