iGEM 2018 — Team Mingdao

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:Mingdao/Notebook

[![](https://static.igem.org/mediawiki/2018/5/5c/T--Mingdao--home_page_icon.png)
HOME](https://2018.igem.org/Team%3AMingdao)

* [PROJECT](https://2018.igem.org/Team%3AMingdao/Description)* [EXPERIMENT](https://2018.igem.org/Team%3AMingdao/Demonstrate)
  * [MODELING](https://2018.igem.org/Team%3AMingdao/Model)
  * [PROTOTYPE](https://2018.igem.org/Team%3AMingdao/Applied_Design)
  * [PARTS](https://2018.igem.org/Team%3AMingdao/Parts)

    + PARTS

    ---

    + [Basic Part](https://2018.igem.org/Team%3AMingdao/Basic_Part)
    + [Composite Part](https://2018.igem.org/Team%3AMingdao/Composite_Part)
    + [Part Collection](https://2018.igem.org/Team%3AMingdao/Part_Collection)
  * [NOTEBOOK](https://2018.igem.org/Team%3AMingdao/Notebook)

    + NOTEBOOK

    ---

    + [Notebook](https://2018.igem.org/Team%3AMingdao/Notebook)
    + [Safety](https://2018.igem.org/Team%3AMingdao/Safety)
  * [HP](https://2018.igem.org/Team%3AMingdao/Human_Practices)

    + HUMAN PRACTICE

    ---

    + [Integrated Human Practices](https://2018.igem.org/Team%3AMingdao/Human_Practices)
    + [Education & Public Engagement](https://2018.igem.org/Team%3AMingdao/Public_Engagement)
    + [Entrepreneurship](https://2018.igem.org/Team%3AMingdao/Entrepreneurship)
  * [TEAM](https://2018.igem.org/Team%3AMingdao/Team)

    + TEAM

    ---

    + [Members](https://2018.igem.org/Team%3AMingdao/Team)
    + [Attributions](https://2018.igem.org/Team%3AMingdao/Attributions)
    + [Collaboration](https://2018.igem.org/Team%3AMingdao/Collaborations)
    + [Interlab](https://2018.igem.org/Team%3AMingdao/InterLab)

* PROJECT

---

* [Project](https://2018.igem.org/Team%3AMingdao/Description)

* EXPERIMENT

---

* [Experiment](https://2018.igem.org/Team%3AMingdao/Experiment)

* MODELING

---

* [Modeling](https://2018.igem.org/Team%3AMingdao/Model)

* PROTOTYPE

---

* [Prototype](https://2018.igem.org/Team%3AMingdao/Applied_Design)

* PARTS

---

* [Basic Part](https://2018.igem.org/Team%3AMingdao/Basic_Part)
* [Composite Part](https://2018.igem.org/Team%3AMingdao/Composite_Part)
* [Part Collection](https://2018.igem.org/Team%3AMingdao/Part_Collection)

* NOTEBOOK

---

* [Notebook](https://2018.igem.org/Team%3AMingdao/Notebook)
* [Safety](https://2018.igem.org/Team%3AMingdao/Safety)

* HP

---

* [Integrated HP](https://2018.igem.org/Team%3AMingdao/Human_Practices)
* [Education & Public Engagement](https://2018.igem.org/Team%3AMingdao/Public_Engagement)
* [Entrepreneurship](https://2018.igem.org/Team%3AMingdao/Entrepreneurship)

* TEAM

---

* [Members](https://2018.igem.org/Team%3AMingdao/Team)
* [Attributions](https://2018.igem.org/Team%3AMingdao/Attributions)
* [Collaboration](https://2018.igem.org/Team%3AMingdao/Collaborations)
* [InterLab](https://2018.igem.org/Team%3AMingdao/InterLab)

Notebook

![](https://static.igem.org/mediawiki/2018/8/85/T--Mingdao--Phil13-7.png)

# Timeline

![](https://static.igem.org/mediawiki/2018/e/e5/T--Mingdao--notebook_timeline_hp.jpg)
![](https://static.igem.org/mediawiki/2018/4/42/T--Mingdao--notebook_timeline_hp2.jpg)

![](https://static.igem.org/mediawiki/2018/6/61/T--Mingdao--notebook_timeline1.jpg)
![](https://static.igem.org/mediawiki/2018/b/bc/T--Mingdao--notebook_timeline4.jpg)
![](https://static.igem.org/mediawiki/2018/4/42/T--Mingdao--notebook_timeline_hp2.jpg)

# Mosquito Assay

![](https://static.igem.org/mediawiki/2018/3/35/T--Mingdao--notebook_flow_chart1.jpg)

# Functional Assay

![](https://static.igem.org/mediawiki/2018/f/fb/T--Mingdao--notebook_flaw_chart2.jpg)

# Plasmid Extraction

![](https://static.igem.org/mediawiki/2018/3/39/T--Mingdao--notebook1.jpg)
![](https://static.igem.org/mediawiki/2018/a/a0/T--Mingdao--notebook2.jpg)

# PCR & Conoly PCR

![](https://static.igem.org/mediawiki/2018/2/2d/T--Mingdao--notebook3.jpg)
![](https://static.igem.org/mediawiki/2018/a/aa/T--Mingdao--notebook4.jpg)

# Gel Elution & PCR Cleanup

![](https://static.igem.org/mediawiki/2018/b/be/T--Mingdao--notebook5.jpg)
![](https://static.igem.org/mediawiki/2018/5/5e/T--Mingdao--notebook6.jpg)

# Restriction Enzyme

![](https://static.igem.org/mediawiki/2018/5/5b/T--Mingdao--notebook7.jpg)

# Transformation

![](https://static.igem.org/mediawiki/2018/d/dc/T--Mingdao--notebook8.jpg)

Notebook

* - Timeline
* - Mosquito Assay
* - Functional Assay
* - Plasmid Extraction
* - PCR & Colony PCR
* - Gel Elution & PCR Cleanup
* - Restriction Enzyme
* - Transformation

![](https://static.igem.org/mediawiki/2018/5/58/T--Mingdao--go_to_top.jpg)

## Quote

*“”*

## Useful Links

[Mingdao High School](http://www.mingdao.edu.tw)

[iGEM](https://igem.org/Main_Page)

## Follow us on

[![](https://image.flaticon.com/icons/svg/87/87892.svg)](https://www.youtube.com/channel/UCaRhx4Mi7XzGwY3J4Pt2YHw)
[![](https://image.flaticon.com/icons/svg/87/87884.svg)](https://www.instagram.com/mingdao_igem_2018/?hl=zh-tw)
[![](https://image.flaticon.com/icons/svg/87/87887.svg)](https://www.facebook.com/mingdaoigem/?ref=bookmarks)

## Contact us

Phone: +886-4-23341797

EMAIL: biolab@ms.mingdao.edu.tw

No.497, Sec. 1, Zhongshan Rd., Wuri District, Taichung City 414, Taiwan (R.O.C.)


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:Mingdao/Experiments

![](https://static.igem.org/mediawiki/2018/4/4f/2018_igem_mobile_menu_logo.svg)

![](https://static.igem.org/mediawiki/igem.org/3/3b/Menu_icon.svg)

[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3AMingdao)
[HOME](https://2018.igem.org/Team%3AMingdao)

TEAM

[Team Members](https://2018.igem.org/Team%3AMingdao/Team)
[Collaborations](https://2018.igem.org/Team%3AMingdao/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AMingdao/Description)
[Design](https://2018.igem.org/Team%3AMingdao/Design)
[Experiments](https://2018.igem.org/Team%3AMingdao/Experiments)
[Notebook](https://2018.igem.org/Team%3AMingdao/Notebook)
[InterLab](https://2018.igem.org/Team%3AMingdao/InterLab)
[Model](https://2018.igem.org/Team%3AMingdao/Model)
[Results](https://2018.igem.org/Team%3AMingdao/Results)
[Demonstrate](https://2018.igem.org/Team%3AMingdao/Demonstrate)
[Improve](https://2018.igem.org/Team%3AMingdao/Improve)
[Attributions](https://2018.igem.org/Team%3AMingdao/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AMingdao/Parts)
[Basic Parts](https://2018.igem.org/Team%3AMingdao/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AMingdao/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AMingdao/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AMingdao/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AMingdao/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AMingdao/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AMingdao/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AMingdao/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AMingdao/Hardware)
[Measurement](https://2018.igem.org/Team%3AMingdao/Measurement)
[Model](https://2018.igem.org/Team%3AMingdao/Model)
[Plant](https://2018.igem.org/Team%3AMingdao/Plant)
[Software](https://2018.igem.org/Team%3AMingdao/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=Mingdao)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:Mingdao/Results

![](https://static.igem.org/mediawiki/2018/4/4f/2018_igem_mobile_menu_logo.svg)

![](https://static.igem.org/mediawiki/igem.org/3/3b/Menu_icon.svg)

[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3AMingdao)
[HOME](https://2018.igem.org/Team%3AMingdao)

TEAM

[Team Members](https://2018.igem.org/Team%3AMingdao/Team)
[Collaborations](https://2018.igem.org/Team%3AMingdao/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AMingdao/Description)
[Design](https://2018.igem.org/Team%3AMingdao/Design)
[Experiments](https://2018.igem.org/Team%3AMingdao/Experiments)
[Notebook](https://2018.igem.org/Team%3AMingdao/Notebook)
[InterLab](https://2018.igem.org/Team%3AMingdao/InterLab)
[Model](https://2018.igem.org/Team%3AMingdao/Model)
[Results](https://2018.igem.org/Team%3AMingdao/Results)
[Demonstrate](https://2018.igem.org/Team%3AMingdao/Demonstrate)
[Improve](https://2018.igem.org/Team%3AMingdao/Improve)
[Attributions](https://2018.igem.org/Team%3AMingdao/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AMingdao/Parts)
[Basic Parts](https://2018.igem.org/Team%3AMingdao/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AMingdao/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AMingdao/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AMingdao/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AMingdao/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AMingdao/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AMingdao/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AMingdao/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AMingdao/Hardware)
[Measurement](https://2018.igem.org/Team%3AMingdao/Measurement)
[Model](https://2018.igem.org/Team%3AMingdao/Model)
[Plant](https://2018.igem.org/Team%3AMingdao/Plant)
[Software](https://2018.igem.org/Team%3AMingdao/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=Mingdao)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)