iGEM 2015 — Team UCLA

# Team:UCLA/Notebook

iGEM UCLA

* [Home](/Team%3AUCLA)
* [Team](/Team%3AUCLA/Team/Meet_the_Team)
  + [Meet the Team](/Team%3AUCLA/Team/Meet_the_Team)
  + [Attributions](/Team%3AUCLA/Attributions)
  + [Collaborations](/Team%3AUCLA/Collaborations)
* [Projects](/Team%3AUCLA/Project)
  + [Programming Spider Silk](/Team%3AUCLA/Project/Programming%20Spider%20Silk)
  + [Functionalizing Silk](/Team%3AUCLA/Project/Functionalizing%20Silk)
  + [Honeybee Silk](/Team%3AUCLA/Project/Honeybee%20Silk)
  + [Materials Processing](/Team%3AUCLA/Project/Protein%20Expression%20and%20Processing)
  + [Protein Cages](/Team%3AUCLA/Project/Protein%20Cages)
  + [Human Practices](/Team%3AUCLA/Practices)
  + [Interlab Study](/Team%3AUCLA/Project/Interlab%20Study)
* [Parts](/Team%3AUCLA/Parts)
  + [Team Parts](/Team%3AUCLA/Parts)
  + [Basic Parts](/Team%3AUCLA/Basic_Part)
  + [Composite Parts](/Team%3AUCLA/Composite_Parts)
  + [Part Collection](/Team%3AUCLA/Part_Collection)
* [Notebook](/Team%3AUCLA/Notebook/Weekly%20Notes)
  + [Programming Spider Silk](/Team%3AUCLA/Notebook/Spider_Silk_Genetics)
  + [Functionalizing Silk](/Team%3AUCLA/Notebook/Recombinant_Expression)
  + [Honeybee Silk](/Team%3AUCLA/Notebook/Honeybee_Silk)
  + [Materials Processing](/Team%3AUCLA/Notebook/Materials)
  + [Protein Cages](/Team%3AUCLA/Notebook/Protein_Cages)
  + [Interlab Study](/Team%3AUCLA/Notebook/Interlab_Study)
* [Safety](/Team%3AUCLA/Safety)
* [Judging](https://igem.org/Judging_Form.cgi?id=1763)

[![](https://static.igem.org/mediawiki/igem.org/1/17/IGEM_basic_Logo_white_stylized.png)](https://2015.igem.org/Main_Page)

## Notebook Template

mm/dd - mm/dd (most recent weeks on top)

mm/dd: Entry title/short description (most recent days on top)

Plain text for descriptions, etc.

Unordered lists for bullet points

For images, upload image onto wiki server. Then replace placeholder image url with your image's url.

![](https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg)

Ordered lists for numbers

For tables, "tr" stands for "table row", and "td" means "table column."

|  |  |  |  |
| --- | --- | --- | --- |
| **Row 1, Column 1** | **Row 1, Column 2** | **Row 1, Column 3** | **Row 1, Column 4** |
| **Row 2, Column 1** | Row 2, Column 2 | Row 2, Column 3 | Row 2, Column 4 |
| **Row 1, Column 1** | Row 2, Column 2 | Row 3, Column 3 | Row 4, Column 4 |

For different types of text styling... **bold**, *italics*, underlined. For headers...

# Headers

## Smaller Header

### You get the point

Feel free to google any other styling.

Keep all your html within the detail tags for the day/week you're putting your entry for.

To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html

mm/dd: Sample Entry

Today we began cloning our GFP.

* PCR'd off template
* Ran gel
* Restriction digest
* Ligated into backbone

For our ligation, we made the following modifications:

1. Tried it with newly bought ligase
2. Left reaction overnight instead of 2 hrs
3. Vector to insert ratio was 1:5 instead of 1:3

PCR Reaction:

|  |  |
| --- | --- |
| **Component** | **Volume** |
| **5X Q5 Reaction Buffer** | 5 |
| **10 mM dNTPs** | 0.5 |
| **10 uM Forward (primer 3/7)** | 1.25 |
| **10 uM Reverse (primer 8)** | 1.25 |
| **Template (diluted to 1ng/uL)** | 0.5 |
| **Q5 High Fidelity DNA Polymerase** | 0.25 |
| **Nuclease Free Water** | 16.25 |

Gel: Lot of bands, all at correct sizes

![](https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg)

mm/dd - mm/dd (most recent weeks on top)

mm/dd: Entry title/short description (most recent days on top)

Plain text for descriptions, etc.

Unordered lists for bullet points

* Bulletpoint 1
* Bulletpoint 2
* Bulletpoint 3

For images, upload image onto wiki server. Then replace placeholder image url with your image's url.

![](https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg)

Ordered lists for numbers

1. Number 1
2. Number 2
3. Number 3

For tables, "tr" stands for "table row", and "td" means "table column."

|  |  |  |  |
| --- | --- | --- | --- |
| **Row 1, Column 1** | **Row 1, Column 2** | **Row 1, Column 3** | **Row 1, Column 4** |
| **Row 2, Column 1** | Row 2, Column 2 | Row 2, Column 3 | Row 2, Column 4 |
| **Row 1, Column 1** | Row 2, Column 2 | Row 3, Column 3 | Row 4, Column 4 |

For different types of text styling... **bold**, *italics*, underlined. For headers...

# Headers

## Smaller Header

### You get the point

Feel free to google any other styling.

Keep all your html within the detail tags for the day/week you're putting your entry for.

To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html

mm/dd: Sample Entry

Today we began cloning our GFP.

* PCR'd off template
* Ran gel
* Restriction digest
* Ligated into backbone

For our ligation, we made the following modifications:

1. Tried it with newly bought ligase
2. Left reaction overnight instead of 2 hrs
3. Vector to insert ratio was 1:5 instead of 1:3

PCR Reaction:

|  |  |
| --- | --- |
| **Component** | **Volume** |
| **5X Q5 Reaction Buffer** | 5 |
| **10 mM dNTPs** | 0.5 |
| **10 uM Forward (primer 3/7)** | 1.25 |
| **10 uM Reverse (primer 8)** | 1.25 |
| **Template (diluted to 1ng/uL)** | 0.5 |
| **Q5 High Fidelity DNA Polymerase** | 0.25 |
| **Nuclease Free Water** | 16.25 |

Gel: Lot of bands, all at correct sizes

![](https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg)

mm/dd - mm/dd (most recent weeks on top)

mm/dd: Entry title/short description (most recent days on top)

Plain text for descriptions, etc.

Unordered lists for bullet points

* Bulletpoint 1
* Bulletpoint 2
* Bulletpoint 3

For images, upload image onto wiki server. Then replace placeholder image url with your image's url.

![](https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg)

Ordered lists for numbers

1. Number 1
2. Number 2
3. Number 3

For tables, "tr" stands for "table row", and "td" means "table column."

|  |  |  |  |
| --- | --- | --- | --- |
| **Row 1, Column 1** | **Row 1, Column 2** | **Row 1, Column 3** | **Row 1, Column 4** |
| **Row 2, Column 1** | Row 2, Column 2 | Row 2, Column 3 | Row 2, Column 4 |
| **Row 1, Column 1** | Row 2, Column 2 | Row 3, Column 3 | Row 4, Column 4 |

For different types of text styling... **bold**, *italics*, underlined. For headers...

# Headers

## Smaller Header

### You get the point

Feel free to google any other styling.

Keep all your html within the detail tags for the day/week you're putting your entry for.

To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html

mm/dd: Sample Entry

Today we began cloning our GFP.

* PCR'd off template
* Ran gel
* Restriction digest
* Ligated into backbone

For our ligation, we made the following modifications:

1. Tried it with newly bought ligase
2. Left reaction overnight instead of 2 hrs
3. Vector to insert ratio was 1:5 instead of 1:3

PCR Reaction:

|  |  |
| --- | --- |
| **Component** | **Volume** |
| **5X Q5 Reaction Buffer** | 5 |
| **10 mM dNTPs** | 0.5 |
| **10 uM Forward (primer 3/7)** | 1.25 |
| **10 uM Reverse (primer 8)** | 1.25 |
| **Template (diluted to 1ng/uL)** | 0.5 |
| **Q5 High Fidelity DNA Polymerase** | 0.25 |
| **Nuclease Free Water** | 16.25 |

Gel: Lot of bands, all at correct sizes

![](https://upload.wikimedia.org/wikipedia/commons/6/60/Gel_electrophoresis_2.jpg)