iGEM 2018 — Team Madrid-OLM

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# Team:Madrid-OLM/Notebook

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[HOME](https://2018.igem.org/Team%3AMadrid-OLM)

TEAM

[Team Members](https://2018.igem.org/Team%3AMadrid-OLM/Team)
[Collaborations](https://2018.igem.org/Team%3AMadrid-OLM/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AMadrid-OLM/Description)
[Design](https://2018.igem.org/Team%3AMadrid-OLM/Design)
[Experiments](https://2018.igem.org/Team%3AMadrid-OLM/Experiments)
[Notebook](https://2018.igem.org/Team%3AMadrid-OLM/Notebook)
[InterLab](https://2018.igem.org/Team%3AMadrid-OLM/InterLab)
[Model](https://2018.igem.org/Team%3AMadrid-OLM/Model)
[Results](https://2018.igem.org/Team%3AMadrid-OLM/Results)
[Demonstrate](https://2018.igem.org/Team%3AMadrid-OLM/Demonstrate)
[Improve](https://2018.igem.org/Team%3AMadrid-OLM/Improve)
[Attributions](https://2018.igem.org/Team%3AMadrid-OLM/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AMadrid-OLM/Parts)
[Basic Parts](https://2018.igem.org/Team%3AMadrid-OLM/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AMadrid-OLM/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AMadrid-OLM/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AMadrid-OLM/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AMadrid-OLM/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AMadrid-OLM/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AMadrid-OLM/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AMadrid-OLM/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AMadrid-OLM/Hardware)
[Measurement](https://2018.igem.org/Team%3AMadrid-OLM/Measurement)
[Model](https://2018.igem.org/Team%3AMadrid-OLM/Model)
[Plant](https://2018.igem.org/Team%3AMadrid-OLM/Plant)
[Software](https://2018.igem.org/Team%3AMadrid-OLM/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=Madrid-OLM)

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:Madrid-OLM/Experiments

Madrid-OLM

[![logo](https://static.igem.org/mediawiki/2018/c/cd/T--Madrid-OLM--verticaLogo.png)](https://2018.igem.org/Team%3AMadrid-OLM)

* Team

  + [Members](https://2018.igem.org/Team%3AMadrid-OLM/Team)
  + [Attribution](https://2018.igem.org/Team%3AMadrid-OLM/Attributions)
  + [Sponsors](https://2018.igem.org/Team%3AMadrid-OLM/Sponsor)
* Project

  + [Description](https://2018.igem.org/Team%3AMadrid-OLM/Description)
  + [Contribution](https://2018.igem.org/Team%3AMadrid-OLM/Contribution)
  + [Improve](https://2018.igem.org/Team%3AMadrid-OLM/Improve)
  + [Demonstrate](https://2018.igem.org/Team%3AMadrid-OLM/Demonstrate)
  + [Judging Form](https://igem.org/2018_Judging_Form?team=Madrid-OLM)
* Aptamers

  + [Overview](https://2018.igem.org/Team%3AMadrid-OLM/Aptamer)
  + [Discovery](https://2018.igem.org/Team%3AMadrid-OLM/AptDiscovery)
  + [Characterization](https://2018.igem.org/Team%3AMadrid-OLM/AptCharacterization)
  + [Computational
     Improvement](https://2018.igem.org/Team%3AMadrid-OLM/Computationaimprovement)
  + [Binding to Electrode](https://2018.igem.org/Team%3AMadrid-OLM/ElectrodeIntegration)
* Device

  + [Overview](https://2018.igem.org/Team%3AMadrid-OLM/OurHardware)
  + [First Prototype](https://2018.igem.org/Team%3AMadrid-OLM/FirstPrototype)
  + Final Prototype

    - [Overview](https://2018.igem.org/Team%3AMadrid-OLM/FinalPrototype)
    - [Microfluidics](https://2018.igem.org/Team%3AMadrid-OLM/HardawareMicrofluidics)
    - [Electronics](https://2018.igem.org/Team%3AMadrid-OLM/HardwareElectronics)
* Experiments

  + [Interlab](https://2018.igem.org/Team%3AMadrid-OLM/InterLab)
  + Protocols

    - [Aptamer Related](https://2018.igem.org/Team%3AMadrid-OLM/AptamerProtocols)
    - [Manufacturing](https://2018.igem.org/Team%3AMadrid-OLM/ProManufacturing)
* Human Practices

  + [Integrated Human Practice](https://2018.igem.org/Team%3AMadrid-OLM/Human_Practices)
  + [Education and
     Engagements](https://2018.igem.org/Team%3AMadrid-OLM/Public_Engagement)
  + [Collaborations](https://2018.igem.org/Team%3AMadrid-OLM/Collaborations)
* Awards

  + [Hardware](https://2018.igem.org/Team%3AMadrid-OLM/Hardware)
  + [Applied Design](https://2018.igem.org/Team%3AMadrid-OLM/Applied_Design)
  + [Entrepreneurship](https://2018.igem.org/Team%3AMadrid-OLM/Entrepreneurship)
  + [Measurement](https://2018.igem.org/Team%3AMadrid-OLM/Measurement)
* [Medals](https://2018.igem.org/Team%3AMadrid-OLM/Medals)

Titulo del apartado

![background](img/wedding-1.jpg)

# Titulo del apartado

Alguna descripcion si queremos

[Posible boton a alguna zona importante](#rsvp)

## Apartado 1

Cosas del apartado 1, se necesita meter mas divs seguramente

## Attendance to Meet Ups

The meets ups bring several advantages to the teams. It is one of the simplest ways to reach a greater number of people to whom you can explain your project. And therefore they allow you to know first-hand the projects of the rest of the teams so that you can consult and clarify doubts. These meetings also allow for more related and powerful collaborations thanks to knowing the rest of the teams in person. And last but not least, they allow you to practice exposing your project with a view to improving in the Giant Jamboree.

Our team attended two meet ups that took place in the month of August.

* Marseille

  ### Mediterranean Meet Up (Marseille)

  ![Image1](https://static.igem.org/mediawiki/2018/9/9a/T--Madrid-OLM--Collaboration--Marseille--All.png)

  This meet up took place between Thursday 9 and Friday 10 August in the city of Marseille and was organized by the team of this city, the Aix-Marseille. In it, all the teams from the Mediterranean area were summoned, so they ended up attending both France and Spain. Francisco, Gonzalo and Ivan attended this meet up on behalf of the team.

  The event began with a reception in which all teams had their first informal contact. Subsequently, a 5 minutes presentation of each of the projects was organized, summarizing the concept on which the proyect was based, the work carried out and the plans to complete it. After the exhibition, each team answered the questions that could have the jury and the rest of the teams.

  ![Image2](https://static.igem.org/mediawiki/2018/4/49/T--Madrid-OLM--Collaboration--Marseille--Exp.png)

  Later they invited us to a joint meal. In the afternoon it was organized a sightseeing tour of the city, but due to inclement weather it had to be suspended, although we could enjoy a pleasant evening of board games and conversations with the rest of the teams. At night a meeting was also organized in a pub to end a day where we had met many new people.

  The second (and last day) had two interesting activities. The first was the meetings with different experts from the university, which privately asked each team about controversial issues. To see if these issues had been taken into account and the possible solutions and consequences were debated.

  ![Image3](https://static.igem.org/mediawiki/2018/2/22/T--Madrid-OLM--Collaboration--Marseille--Experts.png)

  To close the event, a poster presentation session was held, which was very enriching to know how to present our project in Boston..

  One of the things that were achieved in this meet up was the agreement with the UPF-CRG-Barcelona group to organize an activity in their meet up. Activity that is explained in the following session (and that gave rise to a greater collaboration).

  ![Image4](https://static.igem.org/mediawiki/2018/2/2b/T--Madrid-OLM--Collaboration--Marseille--Poster.png)
* Barcelona

  ### Spanish Meet Up (Barcelona)

  ![Image5](https://static.igem.org/mediawiki/2018/6/6d/T--Madrid-OLM--Collaboration--BCN--All.png)

  This meet up took place between Saturday 18 and Monday 20 August in the city of Barcelona and was organized by one of the two teams of this same city, the UPF-CRG-Barcelona. In it, all the teams from Spain were called, which all but the Valencia team attended. To this meet up they were representing the whole team: Laura, Iván and Francisco. The meet up took place in the Biomedical Research Park of Barcelona (PRBB), center where the host team operates and where they started with a welcome activity. This activity consisted of a game where each one of the assistants of each team presented themselves, so that the subsequent environment was much more relaxed.

  The next activity was the one organized by our team. In this activity we proposed to carry out a collaborative guide in which each group would give the main ideas on how they have solved the main problems that have arisen in iGEM. The structure we proposed was followed. We collected these ideas and wrote a complete guide. We explain this collaboration in a later section of the collaborations. After this, some playful activities were carried out on the beach, including volleyball games.

  ![Image6](https://static.igem.org/mediawiki/2018/5/5e/T--Madrid-OLM--Collaboration--BCN--Voley.png)

  During the morning of the second day, a more detailed presentation of each team's project was made. There was 30 minutes to expose each project in detail. This activity was very useful because the rest of the teams, besides getting to know the details of each project well, could intervene to give advice on how to make the presentations. Each team took a series of points in which they could be reinforced in the face of the presentation of the Giant Jamboree.

  In the afternoon the time was taken to take a tour in the tourist center of the city and ended up having dinner with a picnic in the Parc Guell. A viewpoint that have views of the entire city at night.

  ![Image7](https://static.igem.org/mediawiki/2018/7/78/T--Madrid-OLM--Collaboration--BCN--Tour.png)

  During the morning of the last day, to close the meet up, we were able to attend to some enriching expert talks. They offered us their views on synthetic biology and they explained the works in which they are immersed today.

  ![Image8](https://static.igem.org/mediawiki/2018/e/e3/T--Madrid-OLM--Collaboration--BCN--Bye.png)

[Back to Meet up Index](#meetsups)

## Organization of a Meet Up: Another European Meet Up

##### Reason

After attending two meet ups we realized that these meetings are very enriching experiences in preparation for the Giant Jamboree. One of the main reasons for these meet ups is to get to know and know the project of other teams. Other reason is that its also serves to practice the presentations of the project and its results. And finally serve to open the network of contacts beyond the contest itself.

We realized that the events to which more teams come are usually the first ones to be organized, and in these although if they serve to obtain collaborations, do not fulfill so much the function of practicing to present the results of your project due to at the beginning of the summer there are no results.

This year the European meet up was on July 20. Date in which novice teams, without sufficient contacts do not yet know how to deal with these events and do not have the necessary funds to attend. And teams with more experience do not yet have conclusive results. With this context we proposed this ambitious project: Make another European meet up. We invited all the teams of the continent in mid-September. A date that was supposed to be the best for the organization of new teams and with the possibility of simulating the final event.

##### Event

The event was organized between September 14 and 16. Although this event was given as much publicity as possible and contact was made with all possible teams in Europe, attendance was low due to several factors:

* **- Spain is geographically at one end of Europe and travel is expensive.**
* **- The teams were short of funds and were saving for the trip to Boston.**
* **- The event was announced at the beginning of September, many teams did not have room to maneuver in their organization to attend.**

Despite these inconveniences we had assistance from several participants of some teams (all of them came from within the Spanish territory thanks to the facilities for transport). Although there was an extensive program of activities, these had to be adjusted and reorganized so that the maximum number of attendees could enjoy them.

##### Activities

The event began on Friday the 14th in the afternoon with the main course. In it, after the reception of the attendees we gave way to a series of very interesting expert presentations. We were able to count on the attendance of the following personalities

**Victor de Lorenzo**

He is one of the Spanish most relevant researchers in the Synthetic Biology field. He work in the National Biotechnology Center (CNB) a part of the Superior Council of Scientific Investigations (CSIC). His laboratory is specialized in solving environmental issues using genetically engineered Pseudomonas putida. He gave us an interesting talk about *"Synthetic Biology in the rescue of the planet Earth"*.

![Image5](https://static.igem.org/mediawiki/2018/e/ee/T--Madrid-OLM--Collaboration--Madrid--Conference1.png)

**Saul Ares**

He is a researcher of the CNB too. His work is central in the mathematical modeling of Synthetic Biology systems. He has also collaborated with previous iGEM teams. His presentation talked about *"How do cyanobacteria count to ten"*.

![Image5](https://static.igem.org/mediawiki/2018/7/7e/T--Madrid-OLM--Collaboration--Madrid--Conference2.png)

**Krzysztof Wabnik**

He is a Young Investigator Researcher of the Center for Biotechnology and Plant Genomics (CBGP) at the Polytechnic University of Madrid (UPM). The group he is leading uses multilevel computer model simulations, synthetic biology experiments and microfluidics. The name of his presentation was: *"Combining computational and synthetic biology approaches to understand the dynamics of plant hormone signaling circuits"*.

![Image5](https://static.igem.org/mediawiki/2018/2/2d/T--Madrid-OLM--Collaboration--Madrid--Conference3.png)

Despite these inconveniences we had assistance from several participants of some teams (all of them came from within the Spanish territory thanks to the facilities for transport). Although there was an extensive program of activities, these had to be adjusted and reorganized so that the maximum number of attendees could enjoy them.

![Image5](https://static.igem.org/mediawiki/2018/4/4a/T--Madrid-OLM--Collaboration--Madrid--Conference4.png)

After the talks a social event was held in which the attending teams could relax having dinner in the center of Madrid with one of the most typical activities here: go out for tapas.

The second day was inaugurated with a visit to the university to show the facilities where our team works and it was explained which procedures are used in each of the sections that were visited.

Subsequently, the presentations of the projects were made. A simulation of the presentation was carried out as it should be done in Boston, to learn how to take the time and try to correct the excesses or lack of time in each section that was exposed.

In the afternoon there was a tour around Madrid, where the most iconic places of the city were shown. And although there were activities scheduled for the next day, this was the activity that closed the meet up, since the attending teams had organized to return to their cities after this tour.

![Image5](https://static.igem.org/mediawiki/2018/3/30/T--Madrid-OLM--Collaboration--Madrid--Tour.png)

##### Conclusion

It was an event that few people could attend, due to the conditions explained above, but from which the attendees took much advantage. Holding such an event in September could be much more crowded if it was announced from the beginning of the summer and allowed the rest of the teams to organize around it. As it provides a series of very useful tools for the presentation of Boston.

[![](https://static.igem.org/mediawiki/2018/e/ea/T--Madrid-OLM--Inmunoteklogo.png)](http://www.inmunotek.com/en/)

[![](https://static.igem.org/mediawiki/2018/8/81/T--Madrid-OLM--Caixalogo.png)](https://obrasociallacaixa.org/en/)

[![](https://static.igem.org/mediawiki/2018/4/46/T--Madrid-OLM--UCMlogo.png)](https://www.ucm.es/english)

[![](https://static.igem.org/mediawiki/2018/5/50/T--Madrid-OLM--Maquinsalogo.png)](http://www.maquinsa.info/)

[![](https://static.igem.org/mediawiki/2018/1/1e/T--USP-EEL-Brazil--patrocinador5.jpg)](https://eu.idtdna.com/pages)

[![](https://static.igem.org/mediawiki/2018/7/75/T--Madrid-OLM--Biolabslogo.png)](https://international.neb.com/)

[![](https://static.igem.org/mediawiki/2018/c/c7/T--Madrid-OLM--OLMlogo.png)](http://openlabmadrid.es/)

[![](https://static.igem.org/mediawiki/2018/e/ed/T--Madrid-OLM--Ginkgologo.png)](https://www.ucm.es/english)

[![](https://static.igem.org/mediawiki/2018/e/e0/T--Madrid-OLM--Mathworkslogo.png)](https://www.ucm.es/english)

[![](https://static.igem.org/mediawiki/2018/0/01/T--Madrid-OLM--Autodesklogo.png)](https://www.ucm.es/english)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:Madrid-OLM/Results

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[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3AMadrid-OLM)
[HOME](https://2018.igem.org/Team%3AMadrid-OLM)

TEAM

[Team Members](https://2018.igem.org/Team%3AMadrid-OLM/Team)
[Collaborations](https://2018.igem.org/Team%3AMadrid-OLM/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AMadrid-OLM/Description)
[Design](https://2018.igem.org/Team%3AMadrid-OLM/Design)
[Experiments](https://2018.igem.org/Team%3AMadrid-OLM/Experiments)
[Notebook](https://2018.igem.org/Team%3AMadrid-OLM/Notebook)
[InterLab](https://2018.igem.org/Team%3AMadrid-OLM/InterLab)
[Model](https://2018.igem.org/Team%3AMadrid-OLM/Model)
[Results](https://2018.igem.org/Team%3AMadrid-OLM/Results)
[Demonstrate](https://2018.igem.org/Team%3AMadrid-OLM/Demonstrate)
[Improve](https://2018.igem.org/Team%3AMadrid-OLM/Improve)
[Attributions](https://2018.igem.org/Team%3AMadrid-OLM/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AMadrid-OLM/Parts)
[Basic Parts](https://2018.igem.org/Team%3AMadrid-OLM/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AMadrid-OLM/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AMadrid-OLM/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AMadrid-OLM/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AMadrid-OLM/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AMadrid-OLM/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AMadrid-OLM/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AMadrid-OLM/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AMadrid-OLM/Hardware)
[Measurement](https://2018.igem.org/Team%3AMadrid-OLM/Measurement)
[Model](https://2018.igem.org/Team%3AMadrid-OLM/Model)
[Plant](https://2018.igem.org/Team%3AMadrid-OLM/Plant)
[Software](https://2018.igem.org/Team%3AMadrid-OLM/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=Madrid-OLM)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)