iGEM 2017 — Team Peking

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# Team:Peking/Notebook

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[**Peking iGEM**  2017](https://2017.igem.org/Team%3APeking)

[Home](https://2017.igem.org/Team%3APeking)
[Project](https://2017.igem.org/Team%3APeking/Project#Introduction)
[Modelling](https://2017.igem.org/Team%3APeking/Model#Overview)
[Software](https://2017.igem.org/Team%3APeking/Software)
[Hardware](https://2017.igem.org/Team%3APeking/Hardware)
[Lab](https://2017.igem.org/Team%3APeking/Lab)
[Practices](https://2017.igem.org/Team%3APeking/HP)
[Parts](https://2017.igem.org/Team%3APeking/Parts#Overview)
[Team](https://2017.igem.org/Team%3APeking/Team)

# **Notebook**

## Week 1

**26/06/2017-02/07/2017**
Design primers.
Construct standard structure of integrase int5 and int8.

## Week 2

**03/07/2017-09/07/2017**
Mutate integrase int8, int10, p22 for Golden Gate Assembly.
Construct the expression system of integrase TP901, int2, int3, int4, int5, int7, int8, p21, FimB, FimE, HbiF, Vcre, Scre, Cre, Dre, Vika, Lambda-int.
Construct the reporter system utilized the structure of attB-Terminator-attP.

## Week 3

**10/07/2017-16/07/2017**
Mutate Bxb1-gp35, phiC31 for Golden Gate Assembly.
Ask for some plasmids of repressilator.

## Week 4

**17/07/2017-23/07/2017**
Construct the reporter system of Bxb1, TP901, phiC31.
Prepare for repressilator test.

## Week 5

**24/07/2017-30/07/2017**
Construct the expression system of integrase TP901, Bxb1-gp35, phiC31 without RBS.
Design Microfluidic device for the oscillation test of repressilator.

## Week 6

**31/07/2017-06/08/2017**
Construct the expression system of integrase TP901, Bxb1-gp35 with a series RBS of different transcription initiate rate.
Manufacture seven-segment display microfluidic device.

## Week 7

**07/08/2017-13/08/2017**
Construct the expression system of integrase TP901, Bxb1-gp35 with another series RBS of different transcription initiate rate.
Image period of LP117 oscillation around 15 hours by microfluidic device.

## Week 8

**14/08/2017-20/08/2017**
Construct the expression system of integrase phiC31 with a series RBS of different transcription initiate rate.
Manufacture Sand Clock microfluidic device.
Prepare for parts construction.

## Week 9

**21/08/2017-27/08/2017**
Construct the expression system and reporter system of integrase-RDF (recombination directionality factor) fusion protein.
Test integrase recombination efficiency of TP901, Bxb1-gp35, phiC31 by Flow Cytometer.
Image period of plpt20 oscillation around 7 hours.

## Week 10

**28/08/2017-03/09/2017**
Make adjustment for the expression system of integrase TP901, Bxb1-gp35, phiC31 to improve the recombination efficiency, and rebuild the expression system.
Test the adjusted integrase recombination efficiency of TP901, Bxb1-gp35, phiC31 by Flow Cytometer.
Parts construction of integrase TP901.
Site mutation of plpt20 original plasmid.

## Week 11

**04/09/2017-10/09/2017**
Construct the front end of Flip-Flop.
Construct the expression system of integrase-RDF (recombination directionality factor) fusion protein.
Site mutation of plpt20 original plasmid.

## Week 12

**11/09/2017-17/09/2017**
Make adjustment again for the expression system of integrase TP901, Bxb1-gp35, phiC31 to improve the recombination efficiency, and rebuild the expression system.
Test the adjusted integrase recombination efficiency of TP901, Bxb1-gp35, phiC31 by Flow Cytometer.
Construct the reporter system of integrase-RDF (recombination directionality factor) fusion protein.
Parts construction of integrase int2, int5.
Construct the expression system for oscillator plpt20 with integrase Bxb1-gp35.

## Week 13

**18/09/2017-24/09/2017**
Construct TP901, Bxb1, phiC31 expression system of integrase-RDF.
Parts construction of integrase expression system.
Construct the expression system for oscillator plpt20 with integrase T7 promoter.

## Week 14

**25/09/2017-01/10/2017**
Test the recombination efficiency of TP901, Bxb1 integrase-RDF by Flow Cytometer.
Parts construction of integrase reporter system.

## Week 15

**02/10/2017-08/10/2017**
Make adjustment for integrase-RDF expression system to improve recombination efficiency.
Test the recombination efficiency of adjusted integrase-RDF.
Parts construction of integrase expression system and reporter system.
Construct reporter system for oscillator plpt41 with T7 promoter, Bxb1-gp35.
Image repressilator-drived DNA recombination.

## Week 16

**09/10/2017-15/10/2017**
Measure the state transition efficiency of the Forward latch.
Measure the recombination efficiency of the excisionases.
Parts construction of the integrases and excisionases reporter systems.
Measure the flipping efficiency and filter efficiency of the terminators flanked by a pair of attB and attP sites.

## Week 17

**16/10/2017-22/10/2017**
Measure the recombination efficiency of the excisionases.
Parts construction of the integrases and excisionases reporter systems.
Measure the flipping efficiency and filter efficiency of the terminators flanked by two pair of attB and attP sites.

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Goto > Lab/...

[Protocols](https://2017.igem.org/Team%3APeking/Protocols)
[Safety](https://2017.igem.org/Team%3APeking/Safety)
[InterLab](https://2017.igem.org/Team%3APeking/InterLab)

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# Acknowledgement

* [![](https://static.igem.org/mediawiki/2017/9/99/Peking_footer_logo_admin.png)](http://dean.pku.edu.cn/pkudean/)
* [![](https://static.igem.org/mediawiki/2017/a/ab/Peking_footer_logo_slslogo.png)](http://www.bio.pku.edu.cn/en/)
* [![](https://static.igem.org/mediawiki/2017/9/9a/Peking_footer_logo_cqb.png)](http://cqb.pku.edu.cn/en/)

[x]

# Connection

* [Facebook](https://www.facebook.com/pekingigem)
* [Youtube](https://www.youtube.com/channel/UCefEYVCVqKJLmoJrSxivgZQ)
* [Twitter](https://twitter.com/iGEM_Peking)
* [Github](https://github.com/PekingiGEM2017)

[x]

# Contact

* [SynBioWiki.com](http://www.synbiowiki.com)
* Mail

[x]

## **Peking iGEM** 2017

* Peking University,
   No.5 Yiheyuan Road Haidian District, Beijing, P.R.China
  100871

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# Team:Peking/Experiments

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MENU

[HOME](https://2017.igem.org/Team%3APeking)

TEAM

[Team](https://2017.igem.org/Team%3APeking/Team)
[Collaborations](https://2017.igem.org/Team%3APeking/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3APeking/Description)
[Design](https://2017.igem.org/Team%3APeking/Design)
[Experiments](https://2017.igem.org/Team%3APeking/Experiments)
[Notebook](https://2017.igem.org/Team%3APeking/Notebook)
[InterLab](https://2017.igem.org/Team%3APeking/InterLab)
[Contribution](https://2017.igem.org/Team%3APeking/Contribution)
[Model](https://2017.igem.org/Team%3APeking/Model)
[Results](https://2017.igem.org/Team%3APeking/Results)
[Demonstrate](https://2017.igem.org/Team%3APeking/Demonstrate)
[Improve](https://2017.igem.org/Team%3APeking/Improve)
[Attributions](https://2017.igem.org/Team%3APeking/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3APeking/Parts)
[Basic Parts](https://2017.igem.org/Team%3APeking/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3APeking/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3APeking/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3APeking/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3APeking/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3APeking/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3APeking/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3APeking/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3APeking/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3APeking/Hardware)
[Measurement](https://2017.igem.org/Team%3APeking/Measurement)
[Model](https://2017.igem.org/Team%3APeking/Model)
[Plant](https://2017.igem.org/Team%3APeking/Plant)
[Software](https://2017.igem.org/Team%3APeking/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=Peking)

# Peking

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

##### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

##### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

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[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Peking/Results

[![](http://placehold.it/350x150)](https://2017.igem.org/Team%3APeking)

MENU

[HOME](https://2017.igem.org/Team%3APeking)

TEAM

[Team](https://2017.igem.org/Team%3APeking/Team)
[Collaborations](https://2017.igem.org/Team%3APeking/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3APeking/Description)
[Design](https://2017.igem.org/Team%3APeking/Design)
[Experiments](https://2017.igem.org/Team%3APeking/Experiments)
[Notebook](https://2017.igem.org/Team%3APeking/Notebook)
[InterLab](https://2017.igem.org/Team%3APeking/InterLab)
[Contribution](https://2017.igem.org/Team%3APeking/Contribution)
[Model](https://2017.igem.org/Team%3APeking/Model)
[Results](https://2017.igem.org/Team%3APeking/Results)
[Demonstrate](https://2017.igem.org/Team%3APeking/Demonstrate)
[Improve](https://2017.igem.org/Team%3APeking/Improve)
[Attributions](https://2017.igem.org/Team%3APeking/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3APeking/Parts)
[Basic Parts](https://2017.igem.org/Team%3APeking/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3APeking/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3APeking/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3APeking/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3APeking/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3APeking/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3APeking/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3APeking/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3APeking/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3APeking/Hardware)
[Measurement](https://2017.igem.org/Team%3APeking/Measurement)
[Model](https://2017.igem.org/Team%3APeking/Model)
[Plant](https://2017.igem.org/Team%3APeking/Plant)
[Software](https://2017.igem.org/Team%3APeking/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=Peking)

# Peking

# Results

Here you can describe the results of your project and your future plans.

##### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

##### You should also describe what your results mean:

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

##### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

##### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)

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