iGEM 2019 — Team Shanghai_YGQ

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:Shanghai YGQ/Notebook

Shanghai\_YGQ

 [Shanghai\_YGQ](https://2019.igem.org/Team%3AShanghai_YGQ)

![Logo](https://static.igem.org/mediawiki/2019/5/5a/T--Shanghai_YGQ--File_namelogo.jpg)

* [Team](https://2019.igem.org/Team%3AShanghai_YGQ/Team)
  + [Team Member](https://2019.igem.org/Team%3AShanghai_YGQ/Team)
  + [Collaborations](https://2019.igem.org/Team%3AShanghai_YGQ/Collaborations)
* Project
   [Description](https://2019.igem.org/Team%3AShanghai_YGQ/Description)
   [Design](https://2019.igem.org/Team%3AShanghai_YGQ/Design)
   [Experiments](https://2019.igem.org/Team%3AShanghai_YGQ/Experiments)
   [Notebook](https://2019.igem.org/Team%3AShanghai_YGQ/Notebook)
   [Contribution](https://2019.igem.org/Team%3AShanghai_YGQ/Contribution)
   [Results](https://2019.igem.org/Team%3AShanghai_YGQ/Results)
   [Improve](https://2019.igem.org/Team%3AShanghai_YGQ/Improve)
   [Attributions](https://2019.igem.org/Team%3AShanghai_YGQ/Attributions)
* Parts
   [Basic Parts](https://2019.igem.org/Team%3AShanghai_YGQ/Basic_Part)
   [Composite Parts](https://2019.igem.org/Team%3AShanghai_YGQ/Composite_Part)
* [Safety](https://2019.igem.org/Team%3AShanghai_YGQ/Safety)
* [Human Practices](https://2019.igem.org/Team%3AShanghai_YGQ/Human_Practices)
* [Judging Form](https://igem.org/2019_Judging_Form?id=3074)

# Notebook

## Plasmid Design and Validation

### Preparation of Competence

![](https://static.igem.org/mediawiki/2019/8/84/T--Shanghai_YGQ--arrow.jpg)

### Verification of Competence Success

![](https://static.igem.org/mediawiki/2019/8/84/T--Shanghai_YGQ--arrow.jpg)

### Design Primer

![](https://static.igem.org/mediawiki/2019/8/84/T--Shanghai_YGQ--arrow.jpg)

### PCR Amplification Adding Enzyme-cut Site

![](https://static.igem.org/mediawiki/2019/8/84/T--Shanghai_YGQ--arrow.jpg)

### Construct Plasmid

![](https://static.igem.org/mediawiki/2019/8/84/T--Shanghai_YGQ--arrow.jpg)

### Import

Design the gene circuit with the original quorum sensing system of Acetobacter xylinum, and collect data on ginA (Acetobacter xylinum needs electrotransfer)


[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:Shanghai YGQ/Experiments

Shanghai\_YGQ

 [Shanghai\_YGQ](https://2019.igem.org/Team%3AShanghai_YGQ)

![Logo](https://static.igem.org/mediawiki/2019/5/5a/T--Shanghai_YGQ--File_namelogo.jpg)

* [Team](https://2019.igem.org/Team%3AShanghai_YGQ/Team)
  + [Team Member](https://2019.igem.org/Team%3AShanghai_YGQ/Team)
  + [Collaborations](https://2019.igem.org/Team%3AShanghai_YGQ/Collaborations)
* Project
   [Description](https://2019.igem.org/Team%3AShanghai_YGQ/Description)
   [Design](https://2019.igem.org/Team%3AShanghai_YGQ/Design)
   [Experiments](https://2019.igem.org/Team%3AShanghai_YGQ/Experiments)
   [Notebook](https://2019.igem.org/Team%3AShanghai_YGQ/Notebook)
   [Contribution](https://2019.igem.org/Team%3AShanghai_YGQ/Contribution)
   [Results](https://2019.igem.org/Team%3AShanghai_YGQ/Results)
   [Improve](https://2019.igem.org/Team%3AShanghai_YGQ/Improve)
   [Attributions](https://2019.igem.org/Team%3AShanghai_YGQ/Attributions)
* Parts
   [Basic Parts](https://2019.igem.org/Team%3AShanghai_YGQ/Basic_Part)
   [Composite Parts](https://2019.igem.org/Team%3AShanghai_YGQ/Composite_Part)
* [Safety](https://2019.igem.org/Team%3AShanghai_YGQ/Safety)
* [Human Practices](https://2019.igem.org/Team%3AShanghai_YGQ/Human_Practices)
* [Judging Form](https://igem.org/2019_Judging_Form?id=3074)

# Experiment

![](https://static.igem.org/mediawiki/2019/0/0a/T--Shanghai_YGQ--exp.jpg)

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:Shanghai YGQ/Results

Shanghai\_YGQ

 [Shanghai\_YGQ](https://2019.igem.org/Team%3AShanghai_YGQ)

![Logo](https://static.igem.org/mediawiki/2019/5/5a/T--Shanghai_YGQ--File_namelogo.jpg)

* [Team](https://2019.igem.org/Team%3AShanghai_YGQ/Team)
  + [Team Member](https://2019.igem.org/Team%3AShanghai_YGQ/Team)
  + [Collaborations](https://2019.igem.org/Team%3AShanghai_YGQ/Collaborations)
* Project
   [Description](https://2019.igem.org/Team%3AShanghai_YGQ/Description)
   [Design](https://2019.igem.org/Team%3AShanghai_YGQ/Design)
   [Experiments](https://2019.igem.org/Team%3AShanghai_YGQ/Experiments)
   [Notebook](https://2019.igem.org/Team%3AShanghai_YGQ/Notebook)
   [Contribution](https://2019.igem.org/Team%3AShanghai_YGQ/Contribution)
   [Results](https://2019.igem.org/Team%3AShanghai_YGQ/Results)
   [Improve](https://2019.igem.org/Team%3AShanghai_YGQ/Improve)
   [Attributions](https://2019.igem.org/Team%3AShanghai_YGQ/Attributions)
* Parts
   [Basic Parts](https://2019.igem.org/Team%3AShanghai_YGQ/Basic_Part)
   [Composite Parts](https://2019.igem.org/Team%3AShanghai_YGQ/Composite_Part)
* [Safety](https://2019.igem.org/Team%3AShanghai_YGQ/Safety)
* [Human Practices](https://2019.igem.org/Team%3AShanghai_YGQ/Human_Practices)
* [Judging Form](https://igem.org/2019_Judging_Form?id=3074)

# Result

![](https://static.igem.org/mediawiki/2019/0/01/T--Shanghai_YGQ--result1.jpg)
![](https://static.igem.org/mediawiki/2019/b/b0/T--Shanghai_YGQ--result2.jpg)

    The transferred target gene is difficult to form a film, and most of them are flocculent precipitation. The specific reason is unknown. It is speculated that the imported part other than the part in the plasmid may affect it.