iGEM 2015 — Team HUST-China

# Team:HUST-China/Notebook

Team:HUST-China:Description

* [HOME](https://2015.igem.org/Team%3AHUST-China)
* [PROJECT](https://2015.igem.org/Team%3AHUST-China/Project)
  + [Background](https://2015.igem.org/Team%3AHUST-China/Background)
  + [Description](https://2015.igem.org/Team%3AHUST-China/Description)
  + [Design](https://2015.igem.org/Team%3AHUST-China/Design)
* [WETLAB](https://2015.igem.org/Team%3AHUST-China/Wetlab)
  + [Results](https://2015.igem.org/Team%3AHUST-China/Results)
  + [Experiments&protocol](https://2015.igem.org/Team%3AHUST-China/Experiments)
  + [InterLab Study](https://2015.igem.org/Team%3AHUST-China/InterLab%20Study)
  + [Notebook](https://2015.igem.org/Team%3AHUST-China/Notebook)
* [PARTS](https://2015.igem.org/Team%3AHUST-China/Parts)
  + [Basic Parts](https://2015.igem.org/Team%3AHUST-China/Basic_part)
  + [Composite Parts](https://2015.igem.org/Team%3AHUST-China/Basic_part#2)
  + [Part Collection](https://2015.igem.org/Team%3AHUST-China/Part_Collection)
* [MODELING](https://2015.igem.org/Team%3AHUST-China/Modeling)
  + [Overiew](https://2015.igem.org/Team%3AHUST-China/Modeling)
  + [Modeling on Cellular Level](https://2015.igem.org/Team%3AHUST-China/Modeling%20on%20Cellular%20Level)
  + [Modeling on Ecosystem Level](https://2015.igem.org/Team%3AHUST-China/Modeling%20on%20Ecosystem%20Level)
* [HUMAN PRACTICES](https://2015.igem.org/Team%3AHUST-China/Practices)
* [OTHERS](https://2015.igem.org/Team%3AHUST-China/Safety)
  + [Safety](https://2015.igem.org/Team%3AHUST-China/Safety)
  + [Collaborations](https://2015.igem.org/Team%3AHUST-China/Collaborations)
  + [Attributions](https://2015.igem.org/Team%3AHUST-China/Attributions)
  + [Team](https://2015.igem.org/Team%3AHUST-China/Team)
  + [Achievements](https://2015.igem.org/Team%3AHUST-China/Achievements)

![](https://static.igem.org/mediawiki/2015/7/79/HUST_WETLAB_5.PNG)

#### **click it~**

![](https://static.igem.org/mediawiki/2015/8/80/White.png)

![](https://static.igem.org/mediawiki/2015/d/da/Maodian.png)

* [Standalization](#Standalization)
* [Light Control](#Light Control)
* [Viscous Parts](#Viscous Parts)
* [Improvement](#Improvement)
* [Consolidation](#Consolidation)

## Standalization

### Week 1 6.29-7.5

### Week 2 7.6-7.12

### Week 3 7.13-7.19

### Week 4 7.20-7.26

### Week 5 7.27-8.2

### Week 6 8.3-8.9

PCR:

More details, protocol

WangJie Liu and JunJie Xu

![](https://static.igem.org/mediawiki/2015/0/09/Standar-1-form.png)![](https://static.igem.org/mediawiki/2015/f/f6/Standar-1-form-1-pic.png)

![](https://static.igem.org/mediawiki/2015/c/c7/Standar-1-form-2-pic.png)

![](https://static.igem.org/mediawiki/2015/8/8b/Standar-1-form-3-pic.png)

Restriction Enzyme Digestion

WangJie Liu and JunJie Xu

![](https://static.igem.org/mediawiki/2015/4/4c/Standar-2-form.png)

![](https://static.igem.org/mediawiki/2015/0/01/Standar-2-form-2-pic.png)

DNA ligation&Transformation

WangJie Liu and JunJie Xu

![](https://static.igem.org/mediawiki/2015/4/4b/Standar-3-form.png)

Successfully align the sequencing results with the objective sequences:

Colony PCR

WangJie Liu and JunJie Xu

![](https://static.igem.org/mediawiki/2015/f/fd/Standar-4-form.png)
![](https://static.igem.org/mediawiki/2015/c/c5/Standar-4-form-1-pic.png)

![](https://static.igem.org/mediawiki/2015/1/1f/Standar-4-form-2-pic.png)

![](https://static.igem.org/mediawiki/2015/9/90/Standar-4-form-3-pic.png)

![](https://static.igem.org/mediawiki/2015/5/56/Standar-4-form-4-pic.png)

![](https://static.igem.org/mediawiki/2015/6/66/Standar-4-form-5-pic.png)

Extract the plasmid,Conserving.

### Week 7 8.10-8.16

PCR:

More details, protocol

WangJie Liu

![](https://static.igem.org/mediawiki/2015/3/34/Standar-5-form.png)

Restriction Enzyme Digestion

WangJie Liu

![](https://static.igem.org/mediawiki/2015/f/f9/Standar-6-form.png)

![](https://static.igem.org/mediawiki/2015/5/5b/Standar-5-form-1-pic.png)

DNA ligation&Transformation

WangJie Liu

![](https://static.igem.org/mediawiki/2015/9/98/Standar-7-form.png)

Colony PCR

Successfully align the sequencing results with the objective sequences:

WangJie Liu

![](https://static.igem.org/mediawiki/2015/2/2d/Standar-8-form.png)

Extract the plasmid,Conserving.

### Week 8 8.17-8.23

![](https://static.igem.org/mediawiki/2015/a/a7/S-l-W8P1.png)

![](https://static.igem.org/mediawiki/2015/1/1d/S-l-W8P2.png)

![](https://static.igem.org/mediawiki/2015/f/fc/S-l-W8P3.png)

![](https://static.igem.org/mediawiki/2015/a/a1/S-l-W8P4.png)

### Week 9 8.24-8.30

![](https://static.igem.org/mediawiki/2015/2/27/S-l-W9P1.png)

### Week 10 8.31-9.6

Successfully align the sequencing results with the objective sequences

Standalization of BD-CRY2 and AD-CIB1 finished.

![](https://static.igem.org/mediawiki/2015/d/da/S-l-W10P1.png)

![](https://static.igem.org/mediawiki/2015/8/89/S-l-W10P2.png)

![](https://static.igem.org/mediawiki/2015/1/1b/S-l-W10P3.png)

![](https://static.igem.org/mediawiki/2015/4/4a/S-l-W10P4.png)

Successfully align the sequencing results with the objective sequences

Standalization of CRY2 and CIB1 finished.

## Light Control

### Week 1 6.29-7.5

### Week 2 7.6-7.12

![](https://static.igem.org/mediawiki/2015/1/15/L-prt-W2P1.png)

![](https://static.igem.org/mediawiki/2015/1/19/L-prt-W2P2.png)

![](https://static.igem.org/mediawiki/2015/6/64/L-prt-W2P3.png)

![](https://static.igem.org/mediawiki/2015/4/41/L-prt-W2P4.png)
![](https://static.igem.org/mediawiki/2015/4/4f/L-prt-W2P5.png)

Successfully align the sequencing results with the objective sequences of Pgal1 and CYC\_TT.

Extract the plasmid.

Conserving.

### Week 3 7.13-7.19

### Week 4 7.20-7.26

 ![](https://static.igem.org/mediawiki/2015/9/9b/L-prt-W4P1.png)

 ![](https://static.igem.org/mediawiki/2015/8/8d/L-prt-W4P2.png)

![](https://static.igem.org/mediawiki/2015/d/dd/L-prt-W4P3.png)

### Week 5 7.27-8.2

Successfully align the sequencing results with the objective sequences

![](https://static.igem.org/mediawiki/2015/9/94/L-prt-W5P1.png)

![](https://static.igem.org/mediawiki/2015/3/3e/L-prt-W5P2.png)

![](https://static.igem.org/mediawiki/2015/7/71/L-prt-W5P3.png)

![](https://static.igem.org/mediawiki/2015/1/1d/L-prt-W5P4.png)

![](https://static.igem.org/mediawiki/2015/5/5b/L-prt-W5P5.png)

Digestion Test

Guozhao Wu

### Week 6 8.3-8.9

![](https://static.igem.org/mediawiki/2015/8/8a/L-prt-W6P1.png)

![](https://static.igem.org/mediawiki/2015/f/fb/L-prt-W6P2.png)

![](https://static.igem.org/mediawiki/2015/8/8a/L-prt-W6P3.png)

Successfully align the sequencing results with the objective sequences

Pgal-ROX1-CYC\_TT circuit was constructed.

### Week 7 8.10-8.16

### Week 8 8.17-8.23

### Week 9 8.24-8.30

### Week 10 8.31-9.6

## Viscous Parts

### Week 1 6.29-7.5

### Week 2 7.6-7.12

PCR:

More details, protocol

Wang Xi and JunJie Xu

![](https://static.igem.org/mediawiki/2015/0/0e/STMcfp-1-form.png)

![](https://static.igem.org/mediawiki/2015/c/c0/STMcfp-1-form-1-pic.png)

Restriction Enzyme Digestion

Wang Xi and JunJie Xu

![](https://static.igem.org/mediawiki/2015/6/67/STMcfp-2-form.png)

![](https://static.igem.org/mediawiki/2015/9/95/STMcfp-2-form-1-pic.png)

DNA ligation&Transformation

Wang Xi and JunJie Xu

![](https://static.igem.org/mediawiki/2015/f/f9/STMcfp-3-form.png)

Colony PCR

Successfully align the sequencing results with the objective sequences:

Wang Xi and JunJie Xu

![](https://static.igem.org/mediawiki/2015/b/b7/STMcfp-4-form.png)

![](https://static.igem.org/mediawiki/2015/7/71/STMcfp-4-form-1-pic.png)

![](https://static.igem.org/mediawiki/2015/b/bf/STMcfp-4-form-2-pic.png)

Extract the plasmid,Conserving

### Week 3 7.13-7.19

PCR:

More details, protocol

ZhangYu Cheng

![](https://static.igem.org/mediawiki/2015/b/be/STMcfp-5-form.png)

![](https://static.igem.org/mediawiki/2015/a/a0/STMcfp-5-form-1-pic.png)

Restriction Enzyme Digestion

ZhangYu Cheng

![](https://static.igem.org/mediawiki/2015/6/64/STMcfp-6-form.png)

DNA ligation&Transformation

ZhangYu Cheng

![](https://static.igem.org/mediawiki/2015/0/07/STMcfp-7-form.png)

Colony PCR

ZhangYu Cheng

Successfully align the sequencing results with the objective sequences:

![](https://static.igem.org/mediawiki/2015/c/c3/STMcfp-8-form.png)

![](https://static.igem.org/mediawiki/2015/9/93/STMcfp-8-form-1-pic.png)

Extract the plasmid,Conserving.

### Week 4 7.20-7.26

PCR:

More details, protocol

Wang Xi and JunJie Xu

![](https://static.igem.org/mediawiki/2015/c/ce/STMcfp-9-form.png)

Restriction Enzyme Digestion

Wang Xi and JunJie Xu

![](https://static.igem.org/mediawiki/2015/c/cc/STMcfp-10-form.png)

DNA ligation&Transformation

Wang Xi and ZhangYu Cheng

![](https://static.igem.org/mediawiki/2015/e/ec/STMcfp-11-form.png)

Colony PCR

Wang Xi and JunJie Xu

Successfully align the sequencing results with the objective sequences:

![](https://static.igem.org/mediawiki/2015/8/83/STMcfp-12-form.png)

![](https://static.igem.org/mediawiki/2015/f/f2/STMcfp-12-form-1-pic.png)

Extract the plasmid,Conserving.

Tramsformation to yeast

Lei Yin

![](https://static.igem.org/mediawiki/2015/0/0d/STMcfp-13-form.png)

Genome extract and PCR test

Lei Yin

![](https://static.igem.org/mediawiki/2015/c/c8/STMcfp-14-form.png)

Positive strain conservation

### Week 5 7.27-8.2

PCR:

More details, protocol

ZhangYu Cheng

![](https://static.igem.org/mediawiki/2015/4/46/STMcfp-15-form.png)

Restriction Enzyme Digestion

ZhangYu Cheng

![](https://static.igem.org/mediawiki/2015/d/d1/STMcfp-16-form.png)

DNA ligation&Transformation

ZhangYu Cheng

![](https://static.igem.org/mediawiki/2015/2/20/STMcfp-17-form.png)

Colony PCR

ZhangYu Cheng

Successfully align the sequencing results with the objective sequences:

![](https://static.igem.org/mediawiki/2015/6/63/STMcfp-18-form.png)

![](https://static.igem.org/mediawiki/2015/1/17/STMcfp-18-form-1-pic.png)

![](https://static.igem.org/mediawiki/2015/0/0a/STMcfp-18-form-2-pic.png)

![](https://static.igem.org/mediawiki/2015/0/0d/STMcfp-18-form-3-pic.png)

![](https://static.igem.org/mediawiki/2015/a/a0/STMcfp-18-form-4-pic.png)

Extract the plasmid,Conserving.

Trasnsform to yeast

![](https://static.igem.org/mediawiki/2015/a/a4/STMcfp-19-form.png)

Genome extract and PCR test

Lei Yin

![](https://static.igem.org/mediawiki/2015/c/c2/STMcfp-20-form.png)

Positive strain conservation

### Week 6 8.3-8.9

PCR:

More details, protocol

ZhangYu Cheng

![](https://static.igem.org/mediawiki/2015/9/99/STMcfp-21-form.png)

Restriction Enzyme Digestion

ZhangYu Cheng

![](https://static.igem.org/mediawiki/2015/5/5d/STMcfp-22-form.png)

DNA ligation&Transformation

ZhangYu Cheng

![](https://static.igem.org/mediawiki/2015/a/a9/STMcfp-23-form.png)

Colony PCR

ZhangYu Cheng

Successfully align the sequencing results with the objective sequences:

![](https://static.igem.org/mediawiki/2015/e/e9/STMcfp-24-form.png)

![](https://static.igem.org/mediawiki/2015/a/ad/STMcfp-24-form-1-pic.png)

![](https://static.igem.org/mediawiki/2015/a/a8/STMcfp-24-form-2-pic.png)

Extract the plasmid,Conserving.

Trasnsform to yeast

Lei Yin

![](https://static.igem.org/mediawiki/2015/d/d4/STMcfp-25-form.png)

Genome extract and PCR test

Lei Yin

![](https://static.igem.org/mediawiki/2015/2/21/STMcfp-26-form.png)

Positive strain conservation

### Week 7 8.10-8.16

SDS-PAGE

Lei Yin

![](https://static.igem.org/mediawiki/2015/6/65/STMcfp-27-form.png)

Immunoflourecense

ZhangYu Cheng

![](https://static.igem.org/mediawiki/2015/a/ac/STMcfp-28-form.png)

Verification of the secreting Mcfp-3 (Coomassie blue staining)
ZhangYu Cheng

### Week 8 8.17-8.23

### Week 9 8.24-8.30

### Week 10 8.31-9.6

## Improvement

### Week 1 6.29-7.5

### Week 2 7.6-7.12

### Week 3 7.13-7.19

![](https://static.igem.org/mediawiki/2015/3/3f/I-W3P1.png)

![](https://static.igem.org/mediawiki/2015/3/39/I-W3P2.png)

### Week 4 7.20-7.26

### Week 5 7.27-8.2

![](https://static.igem.org/mediawiki/2015/4/49/I-W5P1.png)

![](https://static.igem.org/mediawiki/2015/d/dd/I-W5P2.png)

![](https://static.igem.org/mediawiki/2015/e/e3/I-W5P3.png)

![](https://static.igem.org/mediawiki/2015/3/33/I-W5P4.png)

![](https://static.igem.org/mediawiki/2015/6/6d/I-W5P5.png)

### Week 6 8.3-8.9

![](https://static.igem.org/mediawiki/2015/e/ee/I-W6P1.png)

![](https://static.igem.org/mediawiki/2015/0/02/I-W6P2.png)

![](https://static.igem.org/mediawiki/2015/1/17/I-W6P3.png)

![](https://static.igem.org/mediawiki/2015/d/df/I-W6P4.png)

### Week 7 8.10-8.16

### Week 8 8.17-8.23

### Week 9 8.24-8.30

### Week 10 8.31-9.6

## Consolidation

### Week 1 6.29-7.5

### Week 2 7.6-7.12

### Week 3 7.13-7.19

### Week 4 7.20-7.26

### Week 5 7.27-8.2

### Week 6 8.3-8.9

### Week 7 8.10-8.16

### Week 8 8.17-8.23

### Week 9 8.24-8.30

### Week 10 8.31-9.6

![](https://static.igem.org/mediawiki/2015/6/67/STYZ1.png)

![](https://static.igem.org/mediawiki/2015/d/d4/STYZ2.png)

![](https://static.igem.org/mediawiki/2015/8/82/STYZ3.png)

![](https://static.igem.org/mediawiki/2015/8/88/STYZ4.png)