iGEM 2023 — Team basis-china

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{% block title %}Notebook{% endblock %}
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# Notebook

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[![](https://static.igem.wiki/teams/4880/wiki/project-stock/linker.svg)](#lk1)

* [##### Interactive Notebook](#one)
* [##### Experimental Notes](#two)
* [##### Error Log](#three)

## **Interactive Notebook ///**

Since this is the first time BASIS China has put together an iGEM team, we started recruiting members early, back in May. And we officially formed as a team in the summer of 2022 when we gathered to try and grasp the concepts of Synthetic Biology and iGEM, and take our first lab training session. Following the summer training session, we were often disrupted by COVID-19 regulations, and we couldn't make much progress in the wet lab. However, we did have regular online meetings and analyzed the projects of previous winning iGEM teams. Moving on to February 17, 2023, we started our wet lab work. With our Lab groups A, B, and C, we began constructing and transforming our plasmids with terpenes on a weekly basis. As we made progress with these terpenes, we faced various challenges, some technical and some just foolish (see below), which caused the progress bar to jump back and forth. With more experience under our belts, we made swift progress in our other summer gathering session. It wasn't until the end of September that we ultimately achieved 100% completion on two terpenes: beta-pinene and farnesene.

Date:
<

>
Animate From Beginning

|  |
| --- |
| αPS |

  |  |  |  |  |  |  |  |  |  |  |  |  || βPS |

  |  |  |  |  |  |  |  |  |  |  |  |  || LIMS |

  |  |  |  |  |  |  |  |  |  |  |  |  || PaFS |

  |  |  |  |  |  |  |  |  |  |  |  |  || SaSS |

  |  |  |  |  |  |  |  |  |  |  |  |  || AgBS |

  |  |  |  |  |  |  |  |  |  |  |  |  || PtPS |  |  |  |  |  |  |  |  |  |  |  |  |  |
| LIS |  |  |  |  |  |  |  |  |  |  |  |  |  |
| MsLIMS |  |  |  |  |  |  |  |  |  |  |  |  |  |
| CYP736A167 |  |  |  |  |  |  |  |  |  |  |  |  |  |
|  | plasmid extraction [1] | PCR of target segment [2] | purification [3] | assembly [4] | transformation (into E. coli) [5] | colony PCR [6] | plasmid extraction [7] | sequencing [8] | protein check [9] | transformation (into Syn. 6803) [10] | colony PCR [11] | gas chromatography check [12] | 100% |
|

## **Experimental Notes ///**

## **"Our Foolish Mistakes" ///**

The journey of we high school students from inexperienced messes in the laboratory to individuals capable of performing experiments independently. Many mistakes were made, some humorous, some painful, but we certainly learned from them.

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{% block title %}Engineering Success{% endblock %}
{% block lead %}Demonstrate engineering success in a part of your project by going through at least one iteration of the engineering design cycle.{% endblock %}
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# Engineering Success

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[![](https://static.igem.wiki/teams/4880/wiki/project-stock/linker.svg)](#lk1)

* [##### Cycle 1](#e-coli-plasmid)
* [##### Cycle 2](#cyanobacteria-plasmid)
* [##### Cycle 3](#hardware-design)

## Overview

We always follow the Design-Build-Test-Learn Cycles to move the project forward. This year's project can be roughly divided into three cycles. In the first cycle, we finished building and testing the terpene synthesis circuit in an E. coli chassis. In the second cycle, we achieved to express iteratively terpene synthesis circuit in cyanobacteria, and in the third cycle, we designed kinds of bioreactor to match the engineered cyanobacteria.

![](https://static.igem.wiki/teams/4880/wiki/safety/cycle.png)

Figure 1: Engineering cycle diagram of our project

## **Cycle 1: Terpenes synthesis circuit construction in E.coli ///**

## Design

Due to the fast growth rate of E. coli and the ease of molecular cloning, we initially decided to construct and validate all the terpene production circuit in E. coli. Through gene synthesis, we obtained the target gene, assembled it into plasmid pJA and transformed it into E.coli DH5α.Then, we extracted the plasmid from DH5α and tried to transform and express the terpene synthases in E.coli BL21(DE3).

![](https://static.igem.wiki/teams/4880/wiki/project/plasmid-1.png)
![](https://static.igem.wiki/teams/4880/wiki/project/plasmid-2.png)
![](https://static.igem.wiki/teams/4880/wiki/project/plasmid-3.png)
![](https://static.igem.wiki/teams/4880/wiki/project/plasmid-4.png)
![](https://static.igem.wiki/teams/4880/wiki/project/plasmid-5.png)
![](https://static.igem.wiki/teams/4880/wiki/project/plasmid-6.png)
![](https://static.igem.wiki/teams/4880/wiki/project/plasmid-7.png)
![](https://static.igem.wiki/teams/4880/wiki/project/plasmid-8.png)

Figure 2: Snapgene diagrams of AgBS, αPS, βPS, LIS, MsLIMS, PaFS, PtPS, SaSS, SaSS-CYP736A167

## Build

1. Plasmid Extraction 0802
2. Normal PCR 0804
3. Gel Electrophoresis 0803
4. Assembly 0805
5. E. coli Transformation 0806
6. Colony PCR 0807a & 0807b

![](https://static.igem.wiki/teams/4880/wiki/project/eng1.png)
![](https://static.igem.wiki/teams/4880/wiki/project/eng2.png)
![](https://static.igem.wiki/teams/4880/wiki/project/eng3.png)

Figure 3: Colony PCR results for all our samples

## Test

0.5mM IPTG is added to BL21(DE3) to induce the terpene synthases expression. Through SDS-PAGE, we tested less than only two out of the five tested terpene synthases were be expressed in BL21(DE3).
![](https://static.igem.wiki/teams/4880/wiki/exp/results/sds1.jpg)

Figure 4: SDS-PAGE protein check gel electrophoresis result of AgBS, SaSS, PaFS, αPS

![](https://static.igem.wiki/teams/4880/wiki/exp/results/apinene-gc.png)

Figure 5: Gas chromatography results of terpene production by E.coli(The results of α-Pinene is shown here as an example).

None of the gas chromatography of terpene production by BL21(DE3) have the desired results. As can be seen in Figure 4, the places that have high peaks in the standard product are not the same as the places in our α-pinene.

## Learn

Because less than half of the samples we tested expressed terpene synthases and no samples successfully synthesized terpenes. We needed to find a more efficient way to produce terpenes. Through discussions with our advisorss, we learned that E. coli contains very few of the key precursors for terpene synthesis and is not an ideal chassis for expressing terpenes. Cyanobacteria, although genetically complex to manipulate, are undoubtedly much better at expressing terpene synthases. Therefore, we decided to move on to use cyanobacteria, Synechocystis sp. PCC 6803, as our chassis in the next steps.

## **Cycle 2: Terpenes synthesis circuit construction in Cyanobacteria ///**

## Design

We use electroporation to transform the plasmids we constructed in DH5α E. coli into Synechocystis sp. PCC 6803. After being cultured and induced for a few weeks we add dodecane into the cultures to extract the terpenes, and test whether the terpenes are being produced through gas chromatography.

## Build

1. Cyanobacteria Electroporation Transformation 0809
2. Streaking Cultivation 0810
3. Gel Electrophoresis 0803

![](https://static.igem.wiki/teams/4880/wiki/exp/results/syn1.jpg)
![](https://static.igem.wiki/teams/4880/wiki/exp/results/syn2.jpg)

Figure 6: Streak monocultures of transformed Synechocystis sp. PCC 6803 with terpene synthases genes AgBS, αPS, βPS, LIS, MsLIMS, PaFS, PtPS, SaSS, SaSS-CYP736A167

4. Cyanobacteria Colony PCR 0811

![](https://static.igem.wiki/teams/4880/wiki/exp/results/colony1.png)

Figure 7: Colony PCR of AgBS, βPS, PaFS, PtPS, SaSS in Syn. PCC 6803 monocultures

![](https://static.igem.wiki/teams/4880/wiki/exp/results/colony2.png)

Figure 8: Colony PCR of αPS, SaS-CYP736A167, MsLIMS, LIS in Syn. PCC 6803 monocultures

5. Culture of Monocultures in BG-11 Culture Medium in Erlenmeyer Flask for 2-3 Weeks at 30◦C in Light Bioreactor

## Test

Through gas chromatography, we can see whether the products that our cyanobacteria produced are the same as what we engineered it to produce. Both PaFS and βPS were successful in producing the wanted terpenes. The others are still in the process of testing.

![](https://static.igem.wiki/teams/4880/wiki/project/eng4.jpg)
![](https://static.igem.wiki/teams/4880/wiki/project/eng6.jpg)

Figure 9: picture of induced Syn. PCC 6803 we engineered. The transparent layer at the top is dodecane, which can dissolve the terpene molecules, making them easier to test.

![](https://static.igem.wiki/teams/4880/wiki/exp/results/gc1.png)
![](https://static.igem.wiki/teams/4880/wiki/exp/results/gc2.png)

Figure 10: Gas Chromatography results confirming production of beta pinene and farnasene

## Learn

Though we are able to produce the scent molecules, we are still unable to directly fulfill the purpose of our project, which is to relieve stress through aromatherapy, and to bring our project into people's daily lives. Part of it is because of the safety restrictions of the competition, but even more so is that we do not have a way to do it. From our HPs at our schools, we further confirmed that students face a lot of stress and would really like to have our prospective product in their daily lives. Therefore, we decided to build different bioreactors.

## **Cycle 3: Hardware Design ///**

From the previous two experimental cycles, we have successfully engineered cyanobacteria to produce scents. Our next main cycle will be about building hardware that the cyanobacteria can thrive in. These hardware will be designed so that it can bring greenness and sustainability into the daily lives of people, and lead people out of stress. In this section, we also experienced 3 engineering cycles to get our bioreactor designs to be able used in multiple life contexts.

![](https://static.igem.wiki/teams/4880/wiki/project/module-3-overview.png)

## Module 1

Our project emphasizes greenness and sustainability, with a focus on cyanobacteria, a unique organism independent of organic carbon sources and capable of photosynthesis. To start with designing hardware, we tested cyanobacteria cultivation in common household items like fish tanks or jars, considering factors such as climate, temperature, light, and humidity, based on the different location and family conditions of our team members.

## Module 2

Our first trial in tanks and jars showed cyanobacteria growth relies on a stable environment, which prompts us to create a device that helps with its cultivation and use. The desired apparatus is supposed to maintain a stable environment, provide artificial light and oxygen sources, and allow for proper circulation. A bioreactor, or an apparatus with pumps and transparent tubes which cyanobacteria circulates within may be the solution to maintenance of a stable environment.

## Module 3

In order to produce a new version of the bioreactor that blends well into daily use, our team designed two serials of the bioreactor that have a close resemblance to common large and small items in everyday life. The large bioreactor imitates flowing water sceneries that are common to public facilities and offices. The small bioreactor consists of a wide array of daily items that is correlated with scent. The combination of bioreactors, large and small, provides solutions to anxiety and stress by surrounding us with fragrances.

For more information, please visit [Hardware](https://2023.igem.wiki/basis-china/hardware).

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{% block title %}Experiments{% endblock %}
{% block lead %}Describe the research, experiments, and protocols you used in your iGEM project.{% endblock %}
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![](https://static.igem.wiki/teams/4880/wiki/bkgs/wetlab-2.png)

![](https://static.igem.wiki/teams/4880/wiki/bkgs/wetlab-1.png)

# Experiments

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[![](https://static.igem.wiki/teams/4880/wiki/project-stock/linker.svg)](#lk1)

* [##### Process](#process)
* [##### Protocols](#protocols)

## Experiments Overview

This experiments page includes the protocols we used in our wet lab experiments, and a breakdown of our experiment steps, from acquiring the targeted segment to constructing plasmid in E. coli to transforming the constructed plasmid into cyanobacteria to finally checking the production of the terpenes through GC-MS.

## **Process ///**

* **Plasmid extraction:** extract the plasmid with our wanted segments (terpene synthase genes) from product we bought
* **PCR of targeted segment:** acquire and amplify the segments that we want from the plasmid
* **PCR product purification:** get rid of unwanted DNA, like the original plasmid, and other unwanted substances from PCR product
* **Assembly:** connect the targeted segments to the shuttle vector backbone
* **Transformation into DH5α:** transform the plasmid constructed into competent DH5α E. coli cells
* **Colony PCR:** to see whether the plasmid is transformed into the E. coli or not, and get the rough size of the transformed targeted segment
* **Plasmid extraction:** extract the plasmids from single colonies with positive colony PCR, can be used either for sequencing or for later transformations
* **Sequencing:** send samples to sequencing company and get the exact sequence of constructed plasmid
* **Protein check:**
  + **Normal PCR:** acquire PCR product with terpene synthase genes
  + **Assembly:** assemble PCR product with linearized pET28a plasmid
  + **Transformation into DH5α:** transform the plasmid constructed into competent DH5α E. coli cells
  + **Colony PCR:**to see whether the plasmid is transformed into the E. coli or not, and get the rough size of the transformed targeted segment
  + **Plasmid extraction:**  extract the plasmid for further transformation into BL21(DE3)
  + **Transformation into E. coli BL21(DE3):**transform previously constructed plasmid into BL21(DE3)
  + **Colony PCR:** to see whether the plasmid is transformed into the E. coli or not
  + **Protein induction:** cultivate the positive single colonies, add IPTG to induce the start of transcription, and the expression of protein
  + **SDS-PAGE:** check the size of the proteins that were expressed before and after induction
* **GC-MS check of E. coli product:** to get a chromatogram (chemical composition) showing the products that the proteins have produced
* **Transformation into Synechocystis sp. PCC 6803:** transform the plasmid constructed in DH5α into Syn. PCC 6803
* **Streaking cultivation:** culture positive monocultures and induce them so production of terpenes can be initiated
* **Colony PCR:** to check whether the plasmid has been transformed correctly into Syn. PCC 6803 or not
* **Cultivate and induce monocultures:** culture monocultures that have been transformed successfully and induce them so production of terpenes can be initiated
* **GC-MS check:** to see the products that the Syn. PCC 6803 has produced through chromatogram

![](https://static.igem.wiki/teams/4880/wiki/exp/diagram.png)

## **Protocols ///**

[0802 Plasmid Extraction](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0802-plasmid-extraction.pdf)
[0803 Gel Electrophoresis](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0803-gel-electrophoresis.pdf)
[0804 Normal PCR](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0804-normal-pcr.pdf)
[0805 Assembly](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0805-assembly.pdf)
[0806 E. coli Transformation](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0806-e-coli-transformation.pdf)
[0807a Picking Single Colonies](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0807a-picking-single-colonies.pdf)
[0807b Colony PCR](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0807b-colony-pcr.pdf)
[0808 SDS-PAGE](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0808-sds-page.pdf)
[0809 Cyanobacteria Transformation](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0809-cyanobacteria-transformation.pdf)
[0810 Streaking Cultivation](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0810-streaking-cultivation.pdf)
[0811 Cyanobacteria Colony PCR](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0811-cyanobacteria-colony-pcr.pdf)
[0812 GC-MS](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0812-gc-ms.pdf)
[0901 Biosafety on Agar Plate](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0901-biosafety-on-agar-plate.pdf)
[0902 Biosafety in 24-well Microplate](https://static.igem.wiki/teams/4880/wiki/exp/protocols/0902-biosafety-in-24-well-microplate.pdf)

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{% block lead %}You can describe the results of your project and your future plans here.{% endblock %}
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# Results

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[![](https://static.igem.wiki/teams/4880/wiki/project-stock/linker.svg)](#lk1)

* [##### Verifying the Construction of terpenes produce circuit in E.coli](#plasmid-sequencing)
* [##### Verifying the expression of terpene synthases in E.coli](#e-coli-sds-page)
* [##### Successful Transformation into Synechocystis sp. PCC 6803](#syn-6803-transformation)
* [##### Gas Chromatography](#gas-chromatography)

## Overview

In this section, we will present all the results that are important checkpoints in our experiments. These include successful plasmid construction verification, successful protein expression in E. coli, successful transformation into Syn. PCC 6803, and successful terpene production in Syn. PCC 6803.

## **Verifying the Construction of terpenes produce circuit in E.coli ///**

## Colony PCR of E. Coli Single Colonies

Below are the results of the gel electrophoresis of colony PCR of transformed single DH5alpha E. coli colonies with our constructed plasmids (Figures 1-3). The sizes that are same as the expected size are boxed. The constructed plasmids are made from the assembly of the terpene synthase gene segments and our shuttle vector.

![](https://static.igem.wiki/teams/4880/wiki/exp/results/colony0.png)

Figure 1: The growth of αPS, βPS and LIMS colonies on petri dish

![](https://static.igem.wiki/teams/4880/wiki/exp/results/figure-2.png)

Figure 2: αPS Colony PCR Gel Electrophoresis Results

![](https://static.igem.wiki/teams/4880/wiki/exp/results/figure-3.png)

Figure 3: AgBS, βPS, LIMS, PaFS, PtPS, SaSS Colony PCR Gel Electrophoresis Results

![](https://static.igem.wiki/teams/4880/wiki/exp/results/figure-4.png)

Figure 4: LIS Colony PCR Gel Electrophoresis Results

![](https://static.igem.wiki/teams/4880/wiki/exp/results/figure-5.png)

Figure 5: MsLIMS, LIMS Colony PCR Gel Electrophoresis Results

The correct sizes of all the segments are listed below:

| Terpene Synthase Gene | Expected Size (bp) | Results |
| --- | --- | --- |
| AgBS | 2454 | Successful ✔ |
| αPS | 1884 | Successful ✔ |
| βPS | 1881 | Successful ✔ |
| LIS | 2613 | Successful ✔ |
| LIMS | 1671 | Successful ✔ |
| MsLIMS | 1800 | Successful ✔ |
| PaFS | 1743 | Successful ✔ |
| PtPS | 1845 | Successful ✔ |
| SaSS | 1710 | Successful ✔ |

If the sizes are similar to the correct sizes of the fragments, then there is a chance that the transformation is successful, and the plasmid construction is correct. We will send these positive colony PCR samples to sequencing to test whether they are exactly what we want.

The following genes have at least one positive colony PCR sample: AgBS (Figure 3), αPS (Figure 2), βPS (Figure 3), LIS (Figure 4), LIMS (Figure 5), MsLIMS (Figure 5), PaFS (Figure 3), PtPS (Figure 3), SaSS (Figure 3).

## Sequencing

Sequencing of the plasmids can test whether our constructed plasmid is exactly the same as what we designed and inserted. Below are the diagrams of the sequencing results, the solid red arrows present at the top of the diagrams mean that the sequencing is correct.

![](https://static.igem.wiki/teams/4880/wiki/exp/results/seq1.png)
![](https://static.igem.wiki/teams/4880/wiki/exp/results/seq2.png)
![](https://static.igem.wiki/teams/4880/wiki/exp/results/seq3.png)
![](https://static.igem.wiki/teams/4880/wiki/exp/results/seq4.png)
![](https://static.igem.wiki/teams/4880/wiki/exp/results/seq5.png)
![](https://static.igem.wiki/teams/4880/wiki/exp/results/seq6.png)
![](https://static.igem.wiki/teams/4880/wiki/exp/results/seq7.png)
![](https://static.igem.wiki/teams/4880/wiki/exp/results/seq8.png)
![](https://static.igem.wiki/teams/4880/wiki/exp/results/seq9.png)

Figure 6: Sequencing result Snapgene diagrams of AgBS, αPS, βPS, LIS, MSLIMS, PaFS, PtPS, SaSS, SaSS-CYP736A167

| Terpene Synthase Genes | Results |
| --- | --- |
| AgBS | Successful ✔ |
| αPS | Successful ✔ |
| βPS | Successful ✔ |
| LIS | Successful ✔ |
| LIMS | Unsuccessful × |
| MsLIMS | Successful ✔ |
| PaFS | Successful ✔ |
| PtPS | Successful ✔ |
| SaSS | Successful ✔ |
| SaSS-CYP736A167 | Successful ✔ |

All of our plasmids have positive sequencing (Figure 6) except for LIMS.

## **Verifying the expression of terpene synthases in E.coli ///**

Through SDS-PAGE, we can verify the expression of all the circuits we constructed in E. coli BL21(DE3) with 37◦C, 0.5 mM IPTG. It is regrettable that most proteins, including AgBS, αPS, and MsLIMS, were not expressed. We think that only some of the proteins were expressed because these conditions and the chassis are not optimal for the expression of all the proteins.

![](https://static.igem.wiki/teams/4880/wiki/exp/results/sds1.jpg)

Figure 7: SDS-PAGE protein check gel electrophoresis result of AgBS, SaSS, PaFS, αPS

pET28a-PaFS (Figure 7) and pET28a-SaSS (Figure 7) both have desired protein expression, compared to the control. However, pET28a-AgBS and pET28a-αPS do not have the desired protein expression.

| Terpene Synthase | Protein Size (kDa) | Results |
| --- | --- | --- |
| AgBS | 98.0 | Unsuccessful × |
| αPS | 75.5 | Unsuccessful × |
| MsLIMS | 74.0 | Unsuccessful × |
| PaFS | 70.9 | Successful ✔ |
| SaSS | 69.4 | Successful ✔ |

After discussions within our team and with our advisors, we learned that all the terpene synthases came from plants, and there are some scientists [[1]](#r1b) that used cyanobacteria as the chassis to produce these terpene synthases. This made us more determined to move on to use cyanobacteria as the chassis of our terpene production.

## **Successful Transformation into Synechocystis sp. PCC 6803 ///**

## Colony PCR of Syn. 6803 Single Colonies

![](https://static.igem.wiki/teams/4880/wiki/exp/results/syn1.jpg)
![](https://static.igem.wiki/teams/4880/wiki/exp/results/syn2.jpg)

Figure 8: Streak monocultures of transformed Synechocystis sp. PCC 6803 with terpene synthase genes AgBS, αPS, βPS, LIS, MsLIMS, PaFS, PtPS, SaSS, SaSS-CYP736A167

Below are the gel electrophoresis pictures of the colony PCR of transformed Syn. PCC 6803 with fully constructed plasmids, which are sequencing positive. The single colonies are shown above. If we see that the gene segment is the correct size, then we know that the plasmid is inside the cyanobacteria, and we can begin culturing them for gas chromatography check. The ones that are boxed are all the right sizes.

![](https://static.igem.wiki/teams/4880/wiki/exp/results/figure-7.png)

Figure 9: Colony PCR of AgBS, βPS, PaFS, PtPS, SaSS in Syn. PCC 6803 monocultures

![](https://static.igem.wiki/teams/4880/wiki/exp/results/figure-8.png)

Figure 10: Colony PCR of αPS, SaS-CYP736A167, MsLIMS, LIS in Syn. PCC 6803 monocultures

The correct sizes of all the segments are listed below:

| Terpene Synthase Gene | Expected Size (bp) | Results |
| --- | --- | --- |
| AgBS | 2454 | Successful ✔ |
| αPS | 1884 | Successful ✔ |
| βPS | 1881 | Successful ✔ |
| LIS | 2613 | Unsuccessful × |
| MsLIMS | 1800 | Successful ✔ |
| PaFS | 1743 | Successful ✔ |
| PtPS | 1845 | Successful ✔ |
| SaSS | 1710 | Successful ✔ |
| SaSS-CYP736A167 | 1503 | Successful ✔ |

The following genes have been successfully transformed into Syn. PCC 6803: AgBS (Figure 9), αPS (Figure 10), βPS (Figure 9), LIS (Figure 10), MsLIMS (Figure 10), PaFS (Figure 9), PtPS (Figure 9), SaSS (Figure 9), SaSS-CYP736A167 (Figure 10).

## **Gas Chromatography ///**

Through the help of our advisors, we tested whether the terpenes are being produced by our Syn. PCC 6803 after the inducer is added using gas chromatography. To see whether the test is successful, we check whether there is a peak at the same place as the model sample in the database. Below are the chromatograms of the products that are correct, being beta pinene and farnesene. We are still in the process of testing the other terpenes.

![](https://static.igem.wiki/teams/4880/wiki/exp/results/gc1.png)
![](https://static.igem.wiki/teams/4880/wiki/exp/results/gc2.png)

Figure 9: The GC result of β-pinene and α-Farnesene

## **References ///**

[[1]](#r1) Blanc-Garin, Victoire, et al. “Exploring the Potential of the Model Cyanobacterium Synechocystis PCC 6803 for the Photosynthetic Production of Various High-Value Terpenes.” Biotechnology for Biofuels and Bioproducts, vol. 15, no. 1, 14 Oct. 2022, [DOI](https://doi.org/10.1186/s13068-022-02211-0). Accessed 20 Mar. 2023.

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