iGEM 2021 — Team NCTU_Formosa

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# Team:NCTU Formosa/Notebook

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* Project
  + [Description](https://2021.igem.org/Team%3ANCTU_Formosa/Description)
  + [Design](https://2021.igem.org/Team%3ANCTU_Formosa/Design)
  + [Contribution](https://2021.igem.org/Team%3ANCTU_Formosa/Contribution)
  + [Engineering](https://2021.igem.org/Team%3ANCTU_Formosa/Engineering)
  + [Implementation](https://2021.igem.org/Team%3ANCTU_Formosa/Implementation)
  + [Graphic Design](https://2021.igem.org/Team%3ANCTU_Formosa/Graphic_Design)
  + [Safety](https://2021.igem.org/Team%3ANCTU_Formosa/Safety)
* Results
  + [Experiment Results](https://2021.igem.org/Team%3ANCTU_Formosa/Results)
  + [Proof of Concept](https://2021.igem.org/Team%3ANCTU_Formosa/Proof_Of_Concept)
* [Parts](https://2021.igem.org/Team%3ANCTU_Formosa/Parts)
* Model
  + [Overview](https://2021.igem.org/Team%3ANCTU_Formosa/Model)
  + [Prediction Model](https://2021.igem.org/Team%3ANCTU_Formosa/Prediction_Model)
  + [Efficiency
    Optimization Model](https://2021.igem.org/Team%3ANCTU_Formosa/Efficiency_Optimization_Model)
* Human Practices
  + [Human Practices](https://2021.igem.org/Team%3ANCTU_Formosa/Human_Practices)
  + [Communication](https://2021.igem.org/Team%3ANCTU_Formosa/Communication)
  + [Education](https://2021.igem.org/Team%3ANCTU_Formosa/Education)
  + [Excellence in
    Another Area](https://2021.igem.org/Team%3ANCTU_Formosa/Excellence%20in%20Another%20Area)
* Team
  + [Team Introduction](https://2021.igem.org/Team%3ANCTU_Formosa/Team)
  + [Attributions](https://2021.igem.org/Team%3ANCTU_Formosa/Attributions)
  + [Collaborations](https://2021.igem.org/Team%3ANCTU_Formosa/Collaborations)
  + [Partnership](https://2021.igem.org/Team%3ANCTU_Formosa/Partnership)
* Notebook
  + [Lab Note](https://2021.igem.org/Team%3ANCTU_Formosa/Notebook)
  + [Protocols](https://2021.igem.org/Team%3ANCTU_Formosa/Experiments)
* [Medal](https://2021.igem.org/Team%3ANCTU_Formosa/Criteria)
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Notebook

![](https://static.igem.org/mediawiki/2021/8/89/T--NCTU_Formosa--lab_note.svg)

dark\_mode
Dark Mode

[Jul](#mJul)

[Aug](#mAug)

[Sep](#mSep)

[Oct](#mOct)

| SUN | MON | TUE | WED | THU | FRI | SAT |
| --- | --- | --- | --- | --- | --- | --- |
|  |  |  |  | [1](#July1) | [2](#July2) | [3](#July3) |
| [4](#July4) | [5](#July5) | [6](#July6) | [7](#July7) | [8](#July8) | [9](#July9) | [10](#July10) |
| [11](#July11) | [12](#July12) | [13](#July13) | [14](#July14) | [15](#July15) | [16](#July16) | [17](#July17) |
| [18](#July18) | [19](#July19) | [20](#July20) | [21](#July21) | [22](#July22) | [23](#July23) | [24](#July24) |
| [25](#July25) | [26](#July26) | [27](#July27) | [28](#July28) | [29](#July29) | [30](#July30) | [31](#July31) |

| SUN | MON | TUE | WED | THU | FRI | SAT |
| --- | --- | --- | --- | --- | --- | --- |
| [1](#August1) | [2](#August2) | [3](#August3) | [4](#August4) | [5](#August5) | [6](#August6) | [7](#August7) |
| [8](#August8) | [9](#August9) | [10](#August10) | [11](#August11) | [12](#August12) | [13](#August13) | [14](#August14) |
| [15](#August15) | [16](#August16) | [17](#August17) | [18](#August18) | [19](#August19) | [20](#August20) | [21](#August21) |
| [22](#August22) | [23](#August23) | [24](#August24) | [25](#August25) | [26](#August26) | [27](#August27) | [28](#August28) |
| [29](#August29) | [30](#August30) | [31](#August31) |

| SUN | MON | TUE | WED | THU | FRI | SAT |
| --- | --- | --- | --- | --- | --- | --- |
|  |  |  | [1](#September1) | [2](#September2) | [3](#September3) | [4](#September4) |
| [5](#September5) | [6](#September6) | [7](#September7) | [8](#September8) | [9](#September9) | [10](#September10) | [11](#September11) |
| [12](#September12) | [13](#September13) | [14](#September14) | [15](#September15) | [16](#September16) | [17](#September17) | [18](#September18) |
| [19](#September19) | [20](#September20) | [21](#September21) | [22](#September22) | [23](#September23) | [24](#September24) | [25](#September25) |
| [26](#September26) | [27](#September27) | [28](#September28) | [29](#September29) | [30](#September30) |

| SUN | MON | TUE | WED | THU | FRI | SAT |
| --- | --- | --- | --- | --- | --- | --- |
|  |  |  |  |  | [1](#October1) | [2](#October2) |
| [3](#October3) | [4](#October4) | [5](#October5) | [6](#October6) | [7](#October7) | [8](#October8) | [9](#October9) |
| [10](#October10) | [11](#October11) | [12](#October12) | [13](#October13) | [14](#October14) | [15](#October15) | [16](#October16) |
| [17](#October17) | [18](#October18) | [19](#October19) | [20](#October20) | [21](#October21) | [22](#October22) | [23](#October23) |
| [24](#October24) | [25](#October25) | [26](#October26) | [27](#October27) | [28](#October28) | [29](#October29) | [30](#October30) |
| [31](#October31) |

## July 1

---

None

## July 2

---

None

## July 3

---

None

## July 4

---

None

## July 5

---

None

## July 6

---

None

## July 7

---

None

## July 8

---

None

## July 9

---

None

## July 10

---

None

## July 11

---

None

## July 12

---

None

## July 13

---

None

## July 14

---

None

## July 15

---

None

## July 16

---

None

## July 17

---

None

## July 18

---

None

## July 19

---

None

## July 20

---

None

## July 21

---

None

## July 22

---

None

## July 23

---

None

## July 24

---

None

## July 25

---

None

## July 26

---

None

## July 27

---

None

## July 28

---

None

## July 29

---

Cloning of Sequence A & Sequence B

## July 30

---

Transformation

## July 31

---

None

## August 1

---

Transformation

* Sample:
  + plasmid with ccdB into DH5α
  + plasmid with yafQ into DH5α
  + plasmid with ydfD into DH5α
  + plasmid with hipA into DH5α

Taq PCR & gel electrophoresis

* Sample:LuxI-LuxR, mCherry, ydfD

Start training AI

## August 2

---

None

## August 3

---

Alkaline Lysis & Silica Resin
Cloning of Sequence Test 1

## August 4

---

Digest of Sequence Test 1 & pSB1K3

## August 5

---

Gel electrophoresis & Transformation

## August 6

---

Taq PCR

## August 7

---

None

## August 8

---

None

## August 9

---

Digest of Sequence A & Sequence B & pSB1A3

## August 10

---

Cloning of Sequence Test 2

## August 11

---

Cloning of Sequence C
Digest of Sequence A & Sequence B & pSB1K3

## August 12

---

Gel electrophoresis

## August 13

---

Ligation of sequence A & Sequence B & pSB1K3
Ligation of Sequence Test 2 & pSB1K3

## August 14

---

None

## August 15

---

None

## August 16

---

Digest of Sequence C & pSB1K3-2

## August 17

---

SequenceAB
Seq Test 2

## August 18

---

None

## August 19

---

Digest of Seq A/B/pSB1K3 checking

## August 20

---

Gel electrophoresis

## August 21

---

None

## August 22

---

None

## August 23

---

Phusion PCR of gblock fragment Sequence A & Sequence B & Sequence C

## August 24

---

Culturing

## August 25

---

Ligation of Seq A & pSB1K3

## August 26

---

Seq A/pSB1K3 Transformation

## August 27

---

Gel electrophoresis

## August 28

---

None

## August 29

---

None

## August 30

---

Ligaiton of SeqA/pSB1K3

## August 31

---

Transformation of DH5α/pSB1K3/SeqA

## September 1

---

Taq PCR of Seq A

## September 2

---

Gel electrophoresis ; Culturing ; Taq PCR

## September 3

---

None

## September 4

---

None

## September 5

---

None

## September 6

---

Gel electrophoresis ; Culturing

## September 7

---

Cloning of Seq A/B/C

## September 8

---

Reaction mix of plasmid checking

## September 9

---

None

## September 10

---

Digest of Sequence A/B/C

## September 11

---

None

## September 12

---

None

## September 13

---

None

## September 14

---

None

## September 15

---

Digest of SeqA
Gel electrophoresis
Gel extraction
Ligation
Transformation
Culturing

## September 16

---

Seq Test 1 Culturing
Plasmid extraction
Digest
Gel electrophoresis
sequence B Phusion PCR
Seq A/C/SeqTest1&2 sequencing

## September 17

---

Gel electrophoresis
Culturing

## September 18

---

Plasmid extraction

## September 19

---

Digest of seqA

## September 20

---

Gel electrophoresis
Liquid culturing

## September 21

---

Phusion PCR, digestion of seqA
Colony PCR of seqB
SeqTest1 plasmid extraction gel electrophoresis

## September 22

---

Digestion of SeqTest1
gel electrophoresis
Ligation of seqA

## September 23

---

Transformation of seqA and seqTest2

## September 24

---

Culturing seqA
Colony PCR of seqA

## September 25

---

Liquid culture of seqA
Gel electrophoresis

## September 26

---

Plasmid extraction of seqA
Digestion of seqA

## September 27

---

Phusion PCR of seqC

## September 28

---

Digestion of seqC
Gel electrophoresis

## September 29

---

Digestion of seqC

## September 30

---

Gel electrophoresis
Gel extraction
Transformation of SeqA and SeqC

## October 1

---

Colony PCR and culturing of SeqA + SeqC

## October 2

---

Transformation of SeqTest1
gel electrophoresis
Liquid culture of SeqA + SeqC

## October 3

---

Plasmid extraction of SeqA + SeqC
Gel electrophoresis
Phusion PCR of SeqB

## October 4

---

Digestion of SeqB
Ligaiton of SeqA+SeqB+SeqC

## October 5

---

Colony PCR of SeqB
Transformation of SeqA+SeqB+SeqC

## October 6

---

Colony PCR of SeqA+SeqB+SeqC
Liquid culture of SeqA+SeqB+SeqC

## October 7

---

Gel electrophoresis

## October 8

---

Transformation of SeqA+SeqB+SeqC

## October 9

---

Colony PCR of SeqA+SeqB+SeqC
Liquid culture of SeqA+SeqB+SeqC
Gel electrophoresis

## October 10

---

Plasmid extraction of SeqA+SeqB+SeqC
Digestion of SeqA+SeqB+SeqC

## October 11

---

Gel electrophoresis

## October 12

---

Culture SeqA+SeqB+SeqC

## October 13

---

Gel electrophoresis

## October 14

---

Cell lysis of SeqA+SeqB+SeqC
LL-37 functional test
Transformation of SeqA+SeqB+SeqC

## October 15

---

Gel electrophoresis
Colony PCR of SeqA+SeqB+SeqC

## October 16

---

Biosafety experiment
Gel electrophoresis

## October 17

---

Gel electrophoresis
STATH functional test

## October 18

---

LL-37 functional test
Gel electrophoresis

## October 19

---

Measurement the expression of GFP and RFP

## October 20

---

Safety experiment

## October 21

---

Measurement the expression of GFP and RFP

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# Team:NCTU Formosa/Engineering

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* Results
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* Notebook
  + [Lab Note](https://2021.igem.org/Team%3ANCTU_Formosa/Notebook)
  + [Protocols](https://2021.igem.org/Team%3ANCTU_Formosa/Experiments)
* [Medal](https://2021.igem.org/Team%3ANCTU_Formosa/Criteria)
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NCTU Bi718
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# Team:NCTU Formosa/Experiments

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* Notebook
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* [Medal](https://2021.igem.org/Team%3ANCTU_Formosa/Criteria)
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* Model
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  + [Prediction Model](https://2021.igem.org/Team%3ANCTU_Formosa/Prediction_Model)
  + [Efficiency
    Optimization Model](https://2021.igem.org/Team%3ANCTU_Formosa/Efficiency_Optimization_Model)
* Human Practices
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  + [Communication](https://2021.igem.org/Team%3ANCTU_Formosa/Communication)
  + [Education](https://2021.igem.org/Team%3ANCTU_Formosa/Education)
  + [Excellence in
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* Team
  + [Team Introduction](https://2021.igem.org/Team%3ANCTU_Formosa/Team)
  + [Attributions](https://2021.igem.org/Team%3ANCTU_Formosa/Attributions)
  + [Collaborations](https://2021.igem.org/Team%3ANCTU_Formosa/Collaborations)
  + [Partnership](https://2021.igem.org/Team%3ANCTU_Formosa/Partnership)
* Notebook
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  + [Protocols](https://2021.igem.org/Team%3ANCTU_Formosa/Experiments)
* [Medal](https://2021.igem.org/Team%3ANCTU_Formosa/Criteria)
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