iGEM 2013 — Team KAIT_Japan

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# Team:KAIT Japan/Notebook

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| [![KAIT Japan home.png](/wiki/images/4/43/KAIT_Japan_home.png)](/Team%3AKAIT_Japan "Team:KAIT Japan") [Home](/Team%3AKAIT_Japan "Team:KAIT Japan")  [![KAIT Japan team.png](/wiki/images/f/f1/KAIT_Japan_team.png)](/Team%3AKAIT_Japan/Team "Team:KAIT Japan/Team") [Team](/Team%3AKAIT_Japan/Team "Team:KAIT Japan/Team")  [![KAIT Japan project.png](/wiki/images/9/9a/KAIT_Japan_project.png)](/Team%3AKAIT_Japan/Project "Team:KAIT Japan/Project") [Project](/Team%3AKAIT_Japan/Project "Team:KAIT Japan/Project")  [![KAIT Japan parts.png](/wiki/images/5/59/KAIT_Japan_parts.png)](/Team%3AKAIT_Japan/Parts "Team:KAIT Japan/Parts") [Parts](/Team%3AKAIT_Japan/Parts "Team:KAIT Japan/Parts")  [![KAIT Japan protocol.png](/wiki/images/b/bd/KAIT_Japan_protocol.png)](/Team%3AKAIT_Japan/Protocol "Team:KAIT Japan/Protocol") [Protocol](/Team%3AKAIT_Japan/Protocol "Team:KAIT Japan/Protocol")  ![KAIT_Japan_notebook.png](https://static.igem.org/mediawiki/2013/9/91/KAIT_Japan_notebook.png) **Notebook**  [![KAIT Japan results.png](/wiki/images/8/8e/KAIT_Japan_results.png)](/Team%3AKAIT_Japan/Results "Team:KAIT Japan/Results") [Results](/Team%3AKAIT_Japan/Results "Team:KAIT Japan/Results")  [![KAIT Japan safety.png](/wiki/images/3/32/KAIT_Japan_safety.png)](/Team%3AKAIT_Japan/Safety "Team:KAIT Japan/Safety") [Safety](/Team%3AKAIT_Japan/Safety "Team:KAIT Japan/Safety")  [![KAIT Japan human practice.png](/wiki/images/9/90/KAIT_Japan_human_practice.png)](/Team%3AKAIT_Japan/Human_Practice "Team:KAIT Japan/Human Practice") [Human Practice](/Team%3AKAIT_Japan/Human_Practice "Team:KAIT Japan/Human Practice")   --- | **Notebook** We made an experiment every day. It was process at trial and error. **Basic task** **Date:8/19** Miniprep  **Date:8/20** Miniprep  **Date:8/22** Miniprep  **Date:8/28** Miniprep  **Date:9/3** Miniprep  **Date:9/12** Miniprep **Creating parts of HlyA and pGLO** **Date:8/8** Design of the primer  **Date:8/9** Transformation  **Date:8/12** Subcluture  **Date:8/13** Colony PCR of HlyA and Electrophoresis  **Date:8/16** Transformation,Subcluture and Electrophoresis  **Date:8/22** PCR of HlyA  **Date:8/27** PCR and Electrophoresis  **Date:9/11** The refinement of DNA  **Date:9/11** Infusion **Creating parts of GFP** **Date:8/20** Colony PCR and Electrophoresis  **Date:8/21** Colony PCR and Electrophoresis  **Date:8/23** PCR and Electrophoresis  **Date:8/24** PCR and Electrophoresis  **Date:8/28** PCR  **Date:8/29** PCR and Electrophoresis  **Date:8/30** Subcluture and PCR  **Date:9/5** Subcluture  **Date:9/6** Colony PCR and Electrophoresis  **Date:9/9** PCR  **Date:9/10** PCR  **Date:9/18** PCR and Transformation  **Date:9/19** Colony PCR **Creating parts of IL-10AR,IL-10BR and STAT3** **Date:8/30** Colony PCR and Electrophoresis  **Date:8/31** Colony PCR and Electrophoresis  **Date:9/5** Colony PCR and Electrophoresis of IL-10AR and IL-10BR  **Date:9/6** Colony PCR and Electrophoresis of STAT3  **Date:9/6** PCR and infusion of IL-10AR and IL-10BR  **Date:9/9** Infusion of IL-10R  **Date:9/9** PCR of STAT3  **Date:9/11** Infusion of IL-10R and STAT3 |

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