iGEM 2017 — Team EpiphanyNYC

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:EpiphanyNYC/Notebook

[![](https://hdresolutionigem.com/wp-content/uploads/2017/07/Final-logo-e1501223428664.jpg)](https://2017.igem.org/Team%3AEpiphanyNYC)

[HD Resolution](https://2017.igem.org/Team%3AEpiphanyNYC)

* [Team](https://2017.igem.org/Team%3AEpiphanyNYC/Team)
  + [Team](https://2017.igem.org/Team%3AEpiphanyNYC/Team)
  + [Attributions](https://2017.igem.org/Team%3AEpiphanyNYC/Attributions)
* [Project](https://2017.igem.org/Team%3AEpiphanyNYC/Description)
  + [What is HD?](https://2017.igem.org/Team%3AEpiphanyNYC/WhatIsHD)
  + [Description](https://2017.igem.org/Team%3AEpiphanyNYC/Description)
  + [Design](https://2017.igem.org/Team%3AEpiphanyNYC/Design)
  + [Experiments](https://2017.igem.org/Team%3AEpiphanyNYC/Experiments)
  + [InterLab](https://2017.igem.org/Team%3AEpiphanyNYC/InterLab)
  + [Model](https://2017.igem.org/Team%3AEpiphanyNYC/Model)
  + [Results](https://2017.igem.org/Team%3AEpiphanyNYC/Results)
* Notebook
  + [Lab Notebook](https://2017.igem.org/Team%3AEpiphanyNYC/LabNotebook)
  + [Project Notebook](https://2017.igem.org/Team%3AEpiphanyNYC/Notebook)
* [Human Practices](https://2017.igem.org/Team%3AEpiphanyNYC/HP/Silver)
  + [Human Practices](https://2017.igem.org/Team%3AEpiphanyNYC/HP/Silver)
  + [Integrated Practices](https://2017.igem.org/Team%3AEpiphanyNYC/Gold_Integrated)
  + [Public Engagement](https://2017.igem.org/Team%3AEpiphanyNYC/Engagement)
  + [Collaborations](https://2017.igem.org/Team%3AEpiphanyNYC/Collaborations)

## **Notebook**

Week 1 (March 19-25)

* Consulted with our advisor, Frank, to advise researchers and scientists
* Reached out to scientists at Mt Sinai Hospital, Jason Fuller and Russell Hanson
* Met with Jason Fuller and Russell Hanson, scientists at Genetics Department at Mt Sinai Hospital
* Scheduled weekly meetings for the rest of the year

Week 2 (March 26-Apr 1)

* Brainstormed for a potential topic (Sickle Cell Anemia, Cystic Fibrosis, Huntington’s Disease); decided on HD, proposed by Yeji Cho
* Determined project leaders of the project
* Divided into subsections (website, design, fundraising, social media, computational) and assigned roles, such as other team leads

Week 3 (Apr 2-8)

* Determined possible method to target the disease
* Outlined the abstract for the project

Week 4 (Apr 9-15)

* Took headshots of members and team pictures to put on website
* Administered Drug Tests for medical clearance
* HIPAA (Health Insurance Portability and Accountability Act) Training
* Fire Drill Practice

Week 5 (Apr 16-22)

* Decided to use Wordpress as website template
* Happily received our lab coats and Mount Sinai IDs

Week 6 (Apr 23-29)

* Submitted payment for iGEM group registration
* Signed consent forms

Week 7 (Apr 30-May 6)

* Designed graphic of brain and neuron for website

Week 8 (May 7-13)

* Brainstormed for a team name and designed logo; decided on HD Resolution

Week 9 (May 14-20)

* Designed T-shirts, hats, and sweaters
* Brainstormed for companies/sponsors to partner with us
* Received iGEM parts kit

Week 10 (May 21-27)

* Organized home page of website
* Created sponsorship/partnership letters for possible companies

Week 11 (May 28-Jun 3)

* Julia, Erik and Stacy organized a table at Peace Angels Benefit Concert at Steinway and Sons Concert Hall in May 28, 2017
* Mailed out consent forms
* Scheduled wetlab training dates for the summer

Week 12 (Jun 4-10)

* Early Training: lab safety, project overview, practiced lab techniques such as pipetting, centrifugation, PCR, etc.

Week 13 (Jun 11-17)

* Organized team page of website
* Contacted over 50 private and public companies in pharmaceutical and biotech industries, non-profit organizations for sponsorship and support.

Week 14 (Jun 18-24)

* Created Twitter account
* Christi and Marianne completed safety form 1, 2, 3

Week 15 (Jun 25-Jul 1)

* Jason, Justin, Jeewhan, Marianne met up to analyze the data collection

Week 18 (Jul 16-22)

* Christi, Rachel, Esther, and Marianne organized a fundraising and information table at Dominico-American Society of Queens in Flushing Town Hall in Flushing, Queens NY in July 22 2017
* Created Facebook account
* Created GoFundMe page

Week 19 (Jul 23-29)

* Received over 100 likes on Facebook page thanks to our Social Media and Outreach team.

Week 20 (Jul 30-Aug 5)

* Ordered materials (E. coli, etc)

Week 21 (Aug 6-12)

* Wrote letters to respective schools to ask for partnerships

Week 22 (Aug 13-19)

* Created Instagram account
* Grew cell subcultures of E. Coli for future testing
* Familiarized ourselves with autoclaving and basic sterilizing processes
* Learned how to store E. coli for future experiments
* HDSA agreed to be sponsor

Week 23 (Aug 20-26)

* Learned about transfection and tested cells for competency
* Updated team Wiki’s page on the iGem website
* Reached out to our respective high schools for sponsorship
* Hereditary Disease Foundation/Huntington’s Disease Foundation agreed to be sponsor
* Spread awareness of our project to Alzheimer’s Foundation of America

Week 24 (Aug 27-Sept 2)

* Jessica, Erin, Catherine, and Yunsu helped organize a presentation table at Dominico-American Society of Queens Benefit Concert at National Opera America Center in New York NY on August 30 2017
* Reached out to Cerebral Palsy Alliance Research Foundation about partnering and sponsorship and discussed creating a joint campaign CPARF’s Steptember campaign to promote 10,000 steps a day goal
* Registered team members and created a team roster

Week 25 (Sep 3-9)

* Figured out wet lab schedules and iGEM meetings
* Started introducing each member on Instagram
* Uploaded sponsors and neuron design on website

Week 26 (Sep 10-16)

* Arranged tabs on website
* Sent out emails to organizations for outreach
* Reached out to organizations on Facebook and iGEM teams for collaboration
* Decided lab schedules

Week 27 (Sep 17-23)

* Reached out to respective high schools for presentation
* Tested competency of cells
* Did miniprep to isolate the Addgene HTT plasmids from E. coli
* Created reagents to use for separating plasmid DNA

Week 28 (Sep 24-30)

* Organized project design tab of website
* Completed Safety Lab forms
* Ran gel electrophoresis of Addgene Plasmid Digest

Week 29 (Oct 1-Oct 7)

* Scheduled collaboration and presentation at Columbia University and Stuyvesant High School, respectively
* Cut out Exon1 from Addgene and verified cuts
* Did restriction digest on isolated DNA plasmid

Week 30 (Oct 8-14)

* Created banner for Jamboree
* Started draft for poster
* Did gel extraction and PCR purification
* Purified gel bands from STUL-XBAL digest and amplified isolated DNA fragments

Week 31 (Oct 15-21)

* Jessica, Sohee, Rachel and Eric presented at Huntington’s Disease Society of America headquarters

* Esther, Rachel, Yeji and Eric met with Columbia University Synthetic Biology Initiative and the Columbia iGEM team and presented
* Verified the PCR amplification from October 14th
* Digested samples from October 14th with CAC8I
* Restricted plasmid fragments with CAC8I and ran gel electrophoresis for confirmation
* Invited Kingsborough Community College iGEM team for collaboration

Week 32 (Oct 22-28)

* Rachel and Catherine presented the project at Stuyvesant High School

* Julia, Stacy and Catherine attended the NYC HDSA Family Education Day at Columbia University Medical Center
* Completed judging forms
* Worked on transferring from Wordpress website to wiki

Week 33 (Oct 29-Nov 1)

* Finished transferring Wordpress website content to wiki
* Organized lab notebook and finalized results

![](https://hdresolutionigem.com/wp-content/uploads/2017/07/Final-logo-e1501223428664.jpg)

### HD RESOLUTION

hdresolutionigem@gmail.com | Phone: (347) 709-6167

[![](https://static.igem.org/mediawiki/2017/b/b7/IconFacebook.png)](https://www.facebook.com/HDResolution/)
[![](https://static.igem.org/mediawiki/2017/5/52/IconTwitter.png)](https://twitter.com/HDR_iGEM)
[![](https://static.igem.org/mediawiki/2017/d/dc/IconFund.png)](https://www.gofundme.com/hd-resolution-igem-team)
[![](https://static.igem.org/mediawiki/2017/e/e2/IconInstagram.png)](http://instagram.com/hdr.igem)


[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:EpiphanyNYC/Experiments

[![](https://hdresolutionigem.com/wp-content/uploads/2017/07/Final-logo-e1501223428664.jpg)](https://2017.igem.org/Team%3AEpiphanyNYC)

[HD Resolution](https://2017.igem.org/Team%3AEpiphanyNYC)

* [Team](https://2017.igem.org/Team%3AEpiphanyNYC/Team)
  + [Team](https://2017.igem.org/Team%3AEpiphanyNYC/Team)
  + [Attributions](https://2017.igem.org/Team%3AEpiphanyNYC/Attributions)
* [Project](https://2017.igem.org/Team%3AEpiphanyNYC/Description)
  + [What is HD?](https://2017.igem.org/Team%3AEpiphanyNYC/WhatIsHD)
  + [Description](https://2017.igem.org/Team%3AEpiphanyNYC/Description)
  + [Design](https://2017.igem.org/Team%3AEpiphanyNYC/Design)
  + [Experiments](https://2017.igem.org/Team%3AEpiphanyNYC/Experiments)
  + [InterLab](https://2017.igem.org/Team%3AEpiphanyNYC/InterLab)
  + [Model](https://2017.igem.org/Team%3AEpiphanyNYC/Model)
  + [Results](https://2017.igem.org/Team%3AEpiphanyNYC/Results)
* Notebook
  + [Lab Notebook](https://2017.igem.org/Team%3AEpiphanyNYC/LabNotebook)
  + [Project Notebook](https://2017.igem.org/Team%3AEpiphanyNYC/Notebook)
* [Human Practices](https://2017.igem.org/Team%3AEpiphanyNYC/HP/Silver)
  + [Human Practices](https://2017.igem.org/Team%3AEpiphanyNYC/HP/Silver)
  + [Integrated Practices](https://2017.igem.org/Team%3AEpiphanyNYC/Gold_Integrated)
  + [Public Engagement](https://2017.igem.org/Team%3AEpiphanyNYC/Engagement)
  + [Collaborations](https://2017.igem.org/Team%3AEpiphanyNYC/Collaborations)

## **Experiments**

Transformation Protocol

alt : [Transformation\_Protocol.pdf](https://static.igem.org/mediawiki/2017/b/b7/Transformation_Protocol.pdf)

Casting Gel for Electrophoresis

alt : [Casting\_Gel\_for\_Electrophoresis.pdf](https://static.igem.org/mediawiki/2017/a/a3/Casting_Gel_for_Electrophoresis.pdf)

Ethidum Bromide DNA Gel Staining

alt : [Ethidum\_Bromide\_DNA\_Gel\_Staining.pdf](https://static.igem.org/mediawiki/2017/a/a3/Ethidum_Bromide_DNA_Gel_Staining.pdf)

Flanking Region PCR Reaction

alt : [Flanking\_Region\_PCR\_Reaction.pdf](https://static.igem.org/mediawiki/2017/1/17/Flanking_Region_PCR_Reaction.pdf)

Making Alkaline Lysis Buffer

alt : [Making\_Alkaline\_Lysis\_Buffer.pdf](https://static.igem.org/mediawiki/2017/7/7e/Making_Alkaline_Lysis_Buffer.pdf)

Ligation Protocol WITH T4 DNA Ligase (M0202)

alt : [Ligation\_Protocol\_WITH\_T4\_DNA\_Ligase\_%28M0202%29.pdf](https://static.igem.org/mediawiki/2017/1/15/Ligation_Protocol_WITH_T4_DNA_Ligase_%28M0202%29.pdf)

NEBuilder HiFi DNA Assembly Reaction Protocol

alt : [NEBuilder\_HiFi\_DNA\_Assembly\_Reaction\_Protocol.pdf](https://static.igem.org/mediawiki/2017/5/55/NEBuilder_HiFi_DNA_Assembly_Reaction_Protocol.pdf)

Q5 Hi Fi 2X Master Mix Protocol

alt : [Q5\_Hi\_Fi\_2X\_Master\_Mix\_Protocol.pdf](https://static.igem.org/mediawiki/2017/a/ad/Q5_Hi_Fi_2X_Master_Mix_Protocol.pdf)

Single-temperature Double Digest

alt : [Single-temperature\_Double\_Digest.pdf](https://static.igem.org/mediawiki/2017/d/df/Single-temperature_Double_Digest.pdf)

![](https://hdresolutionigem.com/wp-content/uploads/2017/07/Final-logo-e1501223428664.jpg)

### HD RESOLUTION

hdresolutionigem@gmail.com | Phone: (347) 709-6167

[![](https://static.igem.org/mediawiki/2017/b/b7/IconFacebook.png)](https://www.facebook.com/HDResolution/)
[![](https://static.igem.org/mediawiki/2017/5/52/IconTwitter.png)](https://twitter.com/HDR_iGEM)
[![](https://static.igem.org/mediawiki/2017/d/dc/IconFund.png)](https://www.gofundme.com/hd-resolution-igem-team)
[![](https://static.igem.org/mediawiki/2017/e/e2/IconInstagram.png)](http://instagram.com/hdr.igem)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:EpiphanyNYC/Results

[![](https://hdresolutionigem.com/wp-content/uploads/2017/07/Final-logo-e1501223428664.jpg)](https://2017.igem.org/Team%3AEpiphanyNYC)

[HD Resolution](https://2017.igem.org/Team%3AEpiphanyNYC)

* [Team](https://2017.igem.org/Team%3AEpiphanyNYC/Team)
  + [Team](https://2017.igem.org/Team%3AEpiphanyNYC/Team)
  + [Attributions](https://2017.igem.org/Team%3AEpiphanyNYC/Attributions)
* [Project](https://2017.igem.org/Team%3AEpiphanyNYC/Description)
  + [What is HD?](https://2017.igem.org/Team%3AEpiphanyNYC/WhatIsHD)
  + [Description](https://2017.igem.org/Team%3AEpiphanyNYC/Description)
  + [Design](https://2017.igem.org/Team%3AEpiphanyNYC/Design)
  + [Experiments](https://2017.igem.org/Team%3AEpiphanyNYC/Experiments)
  + [InterLab](https://2017.igem.org/Team%3AEpiphanyNYC/InterLab)
  + [Model](https://2017.igem.org/Team%3AEpiphanyNYC/Model)
  + [Results](https://2017.igem.org/Team%3AEpiphanyNYC/Results)
* Notebook
  + [Lab Notebook](https://2017.igem.org/Team%3AEpiphanyNYC/LabNotebook)
  + [Project Notebook](https://2017.igem.org/Team%3AEpiphanyNYC/Notebook)
* [Human Practices](https://2017.igem.org/Team%3AEpiphanyNYC/HP/Silver)
  + [Human Practices](https://2017.igem.org/Team%3AEpiphanyNYC/HP/Silver)
  + [Integrated Practices](https://2017.igem.org/Team%3AEpiphanyNYC/Gold_Integrated)
  + [Public Engagement](https://2017.igem.org/Team%3AEpiphanyNYC/Engagement)
  + [Collaborations](https://2017.igem.org/Team%3AEpiphanyNYC/Collaborations)

## **Results**

[Results](https://static.igem.org/mediawiki/2017/d/d5/Epres.pdf)
![](https://hdresolutionigem.com/wp-content/uploads/2017/07/Final-logo-e1501223428664.jpg)

### HD RESOLUTION

hdresolutionigem@gmail.com | Phone: (347) 709-6167

[![](https://static.igem.org/mediawiki/2017/b/b7/IconFacebook.png)](https://www.facebook.com/HDResolution/)
[![](https://static.igem.org/mediawiki/2017/5/52/IconTwitter.png)](https://twitter.com/HDR_iGEM)
[![](https://static.igem.org/mediawiki/2017/d/dc/IconFund.png)](https://www.gofundme.com/hd-resolution-igem-team)
[![](https://static.igem.org/mediawiki/2017/e/e2/IconInstagram.png)](http://instagram.com/hdr.igem)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)