iGEM 2014 — Team Freiburg

[![](https://2014.igem.org/images/wiki.png)"](/Main_Page "Main Page")

* [Page](/Team%3AFreiburg/Notebook)
* [Discussion](/wiki/index.php?title=Talk:Team:Freiburg/Notebook&action=edit&redlink=1)
* [View source](/wiki/index.php?title=Team:Freiburg/Notebook&action=edit)
* [History](/wiki/index.php?title=Team:Freiburg/Notebook&action=history)
* teams

* [Log in](/wiki/index.php?title=Special:UserLogin&returnto=Team:Freiburg/Notebook "You are encouraged to log in; however, it is not mandatory [o]")

# Team:Freiburg/Notebook

### From 2014.igem.org

[![iGEM2014 Team Freiburg Logo](https://static.igem.org/mediawiki/2014/4/4e/Header_img.png)](https://2014.igem.org/Team%3AFreiburg)

[The AcCELLerator](https://2014.igem.org/Team%3AFreiburg/Project)

* [Home](/Team%3AFreiburg)
* [Project](/Team%3AFreiburg/Project/Overview)
  + [Overview](/Team%3AFreiburg/Project/Overview)
  + [The Light System](/Team%3AFreiburg/Project/The_light_system)
  + [The Receptor](/Team%3AFreiburg/Project/Receptor)
  + [The Viral Vector](/Team%3AFreiburg/Project/The_viral_vector)
  + [Mammalian Techniques for iGEM](/Team%3AFreiburg/Project/Vision)
  + [Outlook](/Team%3AFreiburg/Project/Outlook)
* [Results](/Team%3AFreiburg/Results)
  + [Summary](/Team%3AFreiburg/Results/Summary)
  + [The Viral Vector](/Team%3AFreiburg/Results/Vector)
  + [The Receptor](/Team%3AFreiburg/Results/Receptor)
  + [The Light System](/Team%3AFreiburg/Results/Light_system)
  + [The Combination](/Team%3AFreiburg/Results/The%20combination)
  + [BioBricks](/Team%3AFreiburg/Results/BioBrick)
  + [Modeling](/Team%3AFreiburg/Results/Modeling)
  + [Light Boxes](/Team%3AFreiburg/Results/Light%20Boxes)
* [Policy and Safety](/Team%3AFreiburg/PolicyAndPractices%20)
  + [Link-It](/Team%3AFreiburg/PolicyAndPractices)
  + [Saturday Balloon Fever](/Team%3AFreiburg/PolicyAndPractices/Saturdayballoonfever)
  + [Online Survey](/Team%3AFreiburg/PolicyAndPractices/survey)
  + [Ethical Essay](/Team%3AFreiburg/PolicyAndPractices/artificialcell)
  + [Interview with Dr. Boldt](/Team%3AFreiburg/PolicyAndPractices/Interview)
  + [Symposium of Biosafety](/Team%3AFreiburg/PolicyAndPractices/Safety/Symposium)
  + [Safety Introduction](/Team%3AFreiburg/PolicyAndPractices/Safety)
  + [Viral Vector Safety](/Team%3AFreiburg/HumanPracticeAndSafety/Safety/viralvector)
  + [Safety-Sheet](/Team%3AFreiburg/PolicyAndPractices/Safety/SafetySheet)
  + [BSL 2 Regulation](/Team%3AFreiburg/PolicyAndPractices/Safety/BiosafetyLvl2)
* [Notebook](/Team%3AFreiburg/Notebook)
  + [Labjournal](/Team%3AFreiburg/Notebook/Labjournal)
  + [Cloning](/Team%3AFreiburg/Notebook/Cloning)
  + [Methods](/Team%3AFreiburg/Notebook/Methods)
* [Team](/Team%3AFreiburg/Team/Members)
  + [Members](/Team%3AFreiburg/Team/Members)
  + [Collaborations](/Team%3AFreiburg/Team/Collaboration)
  + [Judging](/Team%3AFreiburg/Team/Judging)
  + [FUN-Part](/Team%3AFreiburg/Team/FUN-Part)
* [Attributions](/Team%3AFreiburg/Attributions)
  + [Acknowledgement](/Team%3AFreiburg/Attributions/Acknowledgement)+ [Sponsors](/Team%3AFreiburg/Attributions/Sponsors)
* [![iGEM Logo](https://static.igem.org/mediawiki/2014/0/08/Igem_logo.png)](https://2014.igem.org)

# Cloning

The protocols for our methods can be found here.

During our experiments, we conducted a lot of PCRs with different Primers. In order to stay on top of things, we numbered Primers, PCRs and constructs. You can download both lists with detailed information about Primer composition and about PCRs in the following documents:

![](https://static.igem.org/mediawiki/2014/a/a6/2014Freiburg_Labbook_Digests.JPG)

Fig.1: Project overview.

![](https://static.igem.org/mediawiki/2014/e/e7/Freiburg2014_PCRs_labbook.JPG)

Fig.1: Project overview.

## p14rz\_002

* PCR: o14rz\_005, o14\_rz006; Template pQXCIN-SLC7A1
* Digest: pKM006, EcoRI + NotI; PCR-product
* Testdigest with PstI in Cutsmart, expected bands: 5,9; 0,7

![](https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/d/dc/2014Freiburg_140729Testverdau_006%2BSLC%2BHA_PstI_006%2BSLC_PstI.jpg)

Testdigest

## p14rz\_003

* PCR 3
* Digest 3, 4
* Testdigest with PstI + SpeI in Cutsmart, expected bands: 4,1;2,1;1,8

![](https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg)

Testdigest

## p14rz\_004

* PCR 21,22
* Digest -
* Testdigest with PstI in Cutsmart, expected bands: 4,1;3,2

![](https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg)

Testdigest

## p14rz\_005

* PCR 4,5
* Digest 5
* Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,3

![](https://static.igem.org/mediawiki/2014/5/5f/2014Freiburg_140811_PCR1%2C2%2C4%2C5%2C6%2C7%2C9.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg)

Testdigest

## p14rz\_006

* PCR 17,18
* Digest 5
* Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,4

![](https://static.igem.org/mediawiki/2014/5/5e/2014Freiburg_140811_PCR1%2C17%2C18.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg)

Testdigest

## p14rz\_007

* PCR 37,38
* Digest 21
* Testdigest with PstI in Cutsmart, expected bands: 4,1;2,2;1,7

![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/0/00/2014Freiburg_140923_Testverdau_Gibson_16_1x_Gibson_17%2C31%2C33%2C34_je_3x_a.jpg)

Testdigest

## p14rz\_008

* PCR blue light receptor; Template: pQXCIN-SLC7A1
* Digest pKM006 EcoRI, NotI
* Testdigest with SmaI in Cutsmart, expected bands: 5,7

![](https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/c/c8/2014Freiburg_140729Testverdau_084%2BSLC_SmaI.jpg)

Testdigest

## p14rz\_009

* PCR 39,40
* Digest 5
* Testdigest with BamHI in Cutsmart, expected bands: 3,0;2,7

![](https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg)

Testdigest

## p14rz\_010

* PCR 23,24
* Digest 12
* Testdigest with PstI in Cutsmart, expected bands:3,1;2,3;0,56

![](https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/c/c0/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_2_b.jpg)

Testdigest

## p14ls\_003

* PCR 7,9,10
* Digest -
* Testdigest with KpnI in Cutsmart, expected bands: 4,9; 1,3; 0,4

![](https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/6/60/2014Freiburg_140820_PCR_7_2x_PCR_28_2x.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg)

Testdigest

## p14vv\_001

* PCR Primers vv003,vv004; Template: pMIG
* Digest pMIG EcoRI,NotI; C1-eGFP EcoRI,NotI
* Testdigest with NgoMIV in Cutsmart, expected bands: 5,7; 0,6

![](https://static.igem.org/mediawiki/2014/b/b7/2014Freiburg_140719_PCR_fuer_Blaulicht_puro_pKM297_pKM022_pKM292_erfolgreich_55_60_Grad_c1_egfp_plxv_verdau.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140728_Testverdau_pMIG_CMV_1-4_pMIG_MCS_unverdaut2x_verdaut_6x.jpg)

Testdigest

## p14vv\_002

* PCR -
* Digest 8,11
* Testdigest with PstI in Cutsmart, expected bands: 3,2;2,1;1,4

![](https://static.igem.org/mediawiki/2014/0/09/2014Freiburg_140811_PCR10%2C14%2C15%2C17%2C18%2C19%2C20.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/1/19/2014Freiburg_140822_Digest8%2C_11%2C_10.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/5/51/2014Freiburg_140825_Testverdau_rz003_KpnI_2x_pSAM_Xba_3x_ls003_PstI_2x_ls001_Bbs_EcoRI_2x_zl005_EcoRV_5x_pMIG%2BSyn_PstI_5x_pMIG-Xba_Xba%2CSpe_5x_b.jpg)

Testdigest

## p14vv\_003

* PCR pMIG with primer vv003+vv004-
* Digest xx
* Testdigest with KpnI in Cutsmart, expected bands: 3,5;1,5

![](https://static.igem.org/mediawiki/2014/0/06/2014Freiburg_140709_pMIG_vor_Gelelution_3-6b%2CplXV9a-12a.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/1/1f/2014Freiburg_140713_pMIG_IRES_out_und_pMIG_eGFP_und_IRES_out-Testverdau_.jpg)

Testdigest

## p14vv\_006

* PCR 26
* Digest 13
* Testdigest with NgoMIV in Cutsmart, expected bands: 3,5;1,9;0,95,0,6

![](https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/a/a1/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_a.jpg)

Testdigest

## p14vv\_007

* PCR 27
* Digest 14
* Testdigest with NgoMIV in Cutsmart, expected bands: 3,5; 1,3; 0,97; 0,66

![](https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/c/cb/2014Freiburg_9_10_2014_2_13_33_PM_a.jpg)

Testdigest

## p14vv\_008

* PCR 43
* Digest 22
* Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 1,4 0,75

![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg)

Testdigest

## p14vv\_009

* PCR 44
* Digest 22
* Testdigest with EcoRV+MluI in Cutsmart, expected bands: 3,4; 1,43; 0,75

![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg)

Testdigest

## p14vv\_010

* PCR 45
* Digest 22
* Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 2,2

![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg)

Testdigest

## p14vv\_012

* PCR 46
* Digest 22
* Testdigest with EcoRV in Cutsmart, expected bands: 4,7;3,4

![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg)

Testdigest

## p14vv\_013

* PCR 47,48,49
* Digest 22
* Testdigest with NgoMIV in Cutsmart, expected bands: 3,4;2,0;1,3;0,2

![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg)

Testdigest

## p14vv\_014

* PCR 50,51
* Digest 22
* Testdigest with NgoMIV in Cutsmart, expected bands: 4,1; 1,56; 0,66

![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg)

Testdigest

## p14zl\_010

* PCR 1,2
* Digest 1
* Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,4;1,1

![](https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg)

Testdigest

## p14zl\_011

* PCR 19,20
* Digest 1
* Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,0;1,3;0,43

![](https://static.igem.org/mediawiki/2014/b/b0/2014Freiburg_140814_PCR_16%2C19%2C20_Verdau_pLXV_MluI_ClaI_Verdau_pcDNA_BamHI_HindIII.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg)

Testdigest

## p14zl\_016

* PCR 52,53
* Digest 22
* Testdigest with HindIII in Cutsmart, expected bands: 5,7;2,3;1,7

![](https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg)

Testdigest

## p14zl\_017

* PCR 64,65
* Digest 24
* testdigest with NgoMIV in Cutsmart, expected bands: 5,4;2,9

![](https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg)

PCR\_digest

![](https://static.igem.org/mediawiki/2014/b/b3/2014Freiburg_2014_09_14_Testverdaue_Gibson_32_31.jpg)

Testdigest

## p14zl\_020

* PCR 59,60
* Digest 24
* testdigest with BamHI-HF, NgoMIVin Cutsmart, expected bands: 4,7; 2,4; 1,8

![](https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg)

PCR

![](https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg)

Digest

![](https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg)

Testdigest

* [Notebook](#Notebook-Overview)

* [iGEM Team Freiburg 2014](https://2014.igem.org/Team%3AFreiburg/Team/Members)

Inv.

Retrieved from "[http://2014.igem.org/Team:Freiburg/Notebook](http://2014.igem.org/Team%3AFreiburg/Notebook)"

[![Powered by MediaWiki](/wiki/skins/common/images/poweredby_mediawiki_88x31.png)](https://www.mediawiki.org/)

[![Attribution 3.0 Unported](https://i.creativecommons.org/l/by/3.0/88x31.png)](https://creativecommons.org/licenses/by/3.0/)

* [Recent changes](/Special%3ARecentChanges "Recent changes")
* [What links here](/Special%3AWhatLinksHere/Team%3AFreiburg/Notebook "List of all wiki pages that link here [j]")
* [Related changes](/Special%3ARecentChangesLinked/Team%3AFreiburg/Notebook "Recent changes in pages linked from this page [k]")
* [Special pages](/Special%3ASpecialPages "List of all special pages [q]")
* [My preferences](/Special%3APreferences)

* [Printable version](/wiki/index.php?title=Team:Freiburg/Notebook&printable=yes "Printable version of this page [p]")
* [Permanent link](/wiki/index.php?title=Team:Freiburg/Notebook&oldid=288888 "Permanent link to this revision of the page")
* [Privacy policy](/2014.igem.org%3APrivacy_policy "2014.igem.org:Privacy policy")
* [Disclaimers](/2014.igem.org%3AGeneral_disclaimer "2014.igem.org:General disclaimer")