iGEM 2021 — Team IISER_Mohali

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# Team:IISER Mohali/Notebook

![](https://static.igem.org/mediawiki/2021/1/1c/T--IISER_Mohali--logo_lowres.png)

* [Home](https://2021.igem.org/Team%3AIISER_Mohali)
* Project

  + [Description](https://2021.igem.org/Team%3AIISER_Mohali/Description)
  + [Contribution](https://2021.igem.org/Team%3AIISER_Mohali/Contribution)
  + [Engineering](https://2021.igem.org/Team%3AIISER_Mohali/Engineering)
  + [Model](https://2021.igem.org/Team%3AIISER_Mohali/Model)
  + [Hardware](https://2021.igem.org/Team%3AIISER_Mohali/Hardware)
  + [Software](https://2021.igem.org/Team%3AIISER_Mohali/Software)
  + [Proposed Implementation](https://2021.igem.org/Team%3AIISER_Mohali/Proposed_Implementation)
  + [Choice of Fluorophores](https://2021.igem.org/Team%3AIISER_Mohali/Choice_Of_Fluorophores)
  + [Choice of Biomarkers](https://2021.igem.org/Team%3AIISER_Mohali/Choice_Of_Biomarkers)
* [Team](https://2021.igem.org/Team%3AIISER_Mohali/Team)

  + [Attributions](https://2021.igem.org/Team%3AIISER_Mohali/Attributions)
  + [Collaborations](https://2021.igem.org/Team%3AIISER_Mohali/Collaborations)
  + [Partnership](https://2021.igem.org/Team%3AIISER_Mohali/Partnership)
* Experiments

  + [Notebook](https://2021.igem.org/Team%3AIISER_Mohali/Notebook)
* [Human Practices](https://2021.igem.org/Team%3AIISER_Mohali/Human_Practices)

  + [Communication](https://2021.igem.org/Team%3AIISER_Mohali/Communication)
  + [Sustainability](https://2021.igem.org/Team%3AIISER_Mohali/Sustainable)
  + [Entrepreneurship](https://2021.igem.org/Team%3AIISER_Mohali/Entrepreneurship)
  + [Science Education and Communication](https://2021.igem.org/Team%3AIISER_Mohali/Science_Education_and_Communication)
  + [Education and Outreach](https://2021.igem.org/Team%3AIISER_Mohali/Education)
  + [Inclusivity](https://2021.igem.org/Team%3AIISER_Mohali/Inclusivity)
* [Safety](https://2021.igem.org/Team%3AIISER_Mohali/Safety)

Notebooks

[![](https://static.igem.org/mediawiki/2021/1/1c/T--IISER_Mohali--logo_lowres.png)OSCCit](index.html)

# Notebook

---

### Hardware Lab Notebook

---

### Biology Lab Notebook

---

## Get in touch

## Follow

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* © All rights reserved
* Design: [HTML5 UP](http://html5up.net)

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# Team:IISER Mohali/Engineering

![](https://static.igem.org/mediawiki/2021/1/1c/T--IISER_Mohali--logo_lowres.png)

* [Home](https://2021.igem.org/Team%3AIISER_Mohali)
* Project

  + [Description](https://2021.igem.org/Team%3AIISER_Mohali/Description)
  + [Contribution](https://2021.igem.org/Team%3AIISER_Mohali/Contribution)
  + [Engineering](https://2021.igem.org/Team%3AIISER_Mohali/Engineering)
  + [Model](https://2021.igem.org/Team%3AIISER_Mohali/Model)
  + [Hardware](https://2021.igem.org/Team%3AIISER_Mohali/Hardware)
  + [Software](https://2021.igem.org/Team%3AIISER_Mohali/Software)
  + [Proposed Implementation](https://2021.igem.org/Team%3AIISER_Mohali/Proposed_Implementation)
  + [Choice of Fluorophores](https://2021.igem.org/Team%3AIISER_Mohali/Choice_Of_Fluorophores)
  + [Choice of Biomarkers](https://2021.igem.org/Team%3AIISER_Mohali/Choice_Of_Biomarkers)
* [Team](https://2021.igem.org/Team%3AIISER_Mohali/Team)

  + [Attributions](https://2021.igem.org/Team%3AIISER_Mohali/Attributions)
  + [Collaborations](https://2021.igem.org/Team%3AIISER_Mohali/Collaborations)
  + [Partnership](https://2021.igem.org/Team%3AIISER_Mohali/Partnership)
* Experiments

  + [Notebook](https://2021.igem.org/Team%3AIISER_Mohali/Notebook)
* [Human Practices](https://2021.igem.org/Team%3AIISER_Mohali/Human_Practices)

  + [Communication](https://2021.igem.org/Team%3AIISER_Mohali/Communication)
  + [Sustainability](https://2021.igem.org/Team%3AIISER_Mohali/Sustainable)
  + [Entrepreneurship](https://2021.igem.org/Team%3AIISER_Mohali/Entrepreneurship)
  + [Science Education and Communication](https://2021.igem.org/Team%3AIISER_Mohali/Science_Education_and_Communication)
  + [Education and Outreach](https://2021.igem.org/Team%3AIISER_Mohali/Education)
  + [Inclusivity](https://2021.igem.org/Team%3AIISER_Mohali/Inclusivity)
* [Safety](https://2021.igem.org/Team%3AIISER_Mohali/Safety)

Engineering

[![](https://static.igem.org/mediawiki/2021/1/1c/T--IISER_Mohali--logo_lowres.png)OSCCit](index.html)

# Engineering Success

---

This year, we have gone beyond just planning experiments. We have troubleshooted each design aspect of our project, presenting multiple alternatives should they fail. This has been done for both our wet lab and hardware components. We have had great fun in doing this and hope you have fun reading this too! Here’s a PDF of the document shown here.

### Experimental Design Only

---

REFERENCES

1. Falvo E, Arcovito A, Conti G, Cipolla G, Pitea M, Morea V, Damiani V, Sala G, Fracasso G, Ceci P. Engineered Human Nanoferritin Bearing the Drug Genz-644282 for Cancer Therapy. Pharmaceutics. 2020 Oct 20;12(10):992. doi: 10.3390/pharmaceutics12100992. PMID: 33092088; PMCID: PMC7589674.- Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin; Caleb Cassidy-Amstutz, Luke Oltrogge, Catherine C. Going, Antony Lee, Poh Teng, David Quintanilla, Alexandra East-Seletsky, Evan R. Williams, and David F. Savage Biochemistry **2016** *55* (24), 3461-3468 DOI: 10.1021/acs.biochem.6b00294- Lee, T-H, Carpenter, TS, D'haeseleer, P, Savage, DF, Yung, MC. Encapsulin carrier proteins for enhanced expression of antimicrobial peptides. *Biotechnology and Bioengineering*. 2020; 117: 603– 613.<https://doi.org/10.1002/bit.27222>- Jones JA, Giessen TW. Advances in encapsulin nanocompartment biology and engineering. *Biotechnol Bioeng*. 2021;118(1):491-505. doi:10.1002/bit.27564

## Get in touch

## Follow

![](https://static.igem.org/mediawiki/2021/4/4c/T--UPenn--idt.png)

![](https://static.igem.org/mediawiki/2021/8/88/T--UNSW_Australia--img--sponsor_logos--frederick.png)

![](https://static.igem.org/mediawiki/2021/8/83/T--IISER_Mohali--DBT.png)

![](https://static.igem.org/mediawiki/2021/c/c3/T--IISER_Mohali--IISERM.png)

* © All rights reserved
* Design: [HTML5 UP](http://html5up.net)

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:IISER Mohali/Experiments

IISER\_Mohali

# [--Project Title--](https://2021.igem.org/wiki/index.php?title=Template:IISER_Mohali&action=raw&ctype=text/html)

IISER Mohali

* [Home](https://2021.igem.org/wiki/index.php?title=Template:IISER_Mohali&action=raw&ctype=text/html)
* Project
  + Description
  + Contribution
  + Engineering
  + Model
  + Proof of Concept
  + Hardware
* Team
  + Attributions
  + Collaborations
  + Partnerships
* Experiments
  + Dry Lab
  + Wet Lab
  + Notebook
  + Measurements
* Human Practices
  + Education
  + Inclusivity
  + Communication
  + Implementation
  + Entrepreneurship
  + Sustainable Development
* [Results](https://2021.igem.org/wiki/index.php?title=Template:IISER_Mohali&action=raw&ctype=text/html)
* [Safety](https://2021.igem.org/wiki/index.php?title=Template:IISER_Mohali&action=raw&ctype=text/html)

The Introduction

So in case you were wondering what this is all about ...

Escape Velocity is a free responsive
site template by [HTML5 UP](http://html5up.net)

It's **responsive**, built on **HTML5** and **CSS3**, and released for
free under the [Creative Commons Attribution 3.0 license](http://html5up.net/license), so use it for any of
your personal or commercial projects – just be sure to credit us!

* Proceed

The Details

![](https://static.igem.org/mediawiki/2021/1/1d/T--IISER_Mohali--pic01.jpg)

## Dolor consequat feugiat amet veroeros

Feugiat dolor nullam orci pretium phasellus justo

### Mattis velit diam vulputate

Eget mattis at, laoreet vel et velit aliquam diam ante, aliquet sit amet vulputate et magna feugiat laoreet vel velit lorem.

### Lorem ipsum dolor sit veroeros

Eget mattis at, laoreet vel et velit aliquam diam ante, aliquet sit amet vulputate et magna feugiat laoreet vel velit lorem.

### Pretium phasellus justo lorem

Eget mattis at, laoreet vel et velit aliquam diam ante, aliquet sit amet vulputate et magna feugiat laoreet vel velit lorem.

### Tempus sed pretium orci

Eget mattis at, laoreet vel et velit aliquam diam ante, aliquet sit amet vulputate et magna feugiat laoreet vel velit lorem.

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* Get Started
* More Info

The Endorsements

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* Learn More

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* Learn More

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* Learn More

The Rest Of It

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Nam pharetra laoreet imperdiet volutpat etiam feugiat.

### Mailing Address

Untitled Corp
1234 Somewhere Rd
Nashville, TN 00000

### Social

@untitled-corp
linkedin.com/untitled
facebook.com/untitled

### Email

info@untitled.tld

### Phone

(000) 555-0000

* © Untitled.
* Design: [HTML5 UP](http://html5up.net)

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:IISER Mohali/Results

IISER\_Mohali

# [--Project Title--](https://2021.igem.org/wiki/index.php?title=Template:IISER_Mohali&action=raw&ctype=text/html)

IISER Mohali

* [Home](https://2021.igem.org/wiki/index.php?title=Template:IISER_Mohali&action=raw&ctype=text/html)
* Project
  + Description
  + Contribution
  + Engineering
  + Model
  + Proof of Concept
  + Hardware
* Team
  + Attributions
  + Collaborations
  + Partnerships
* Experiments
  + Dry Lab
  + Wet Lab
  + Notebook
  + Measurements
* Human Practices
  + Education
  + Inclusivity
  + Communication
  + Implementation
  + Entrepreneurship
  + Sustainable Development
* [Results](https://2021.igem.org/wiki/index.php?title=Template:IISER_Mohali&action=raw&ctype=text/html)
* [Safety](https://2021.igem.org/wiki/index.php?title=Template:IISER_Mohali&action=raw&ctype=text/html)

The Introduction

So in case you were wondering what this is all about ...

Escape Velocity is a free responsive
site template by [HTML5 UP](http://html5up.net)

It's **responsive**, built on **HTML5** and **CSS3**, and released for
free under the [Creative Commons Attribution 3.0 license](http://html5up.net/license), so use it for any of
your personal or commercial projects – just be sure to credit us!

* Proceed

The Details

![](https://static.igem.org/mediawiki/2021/1/1d/T--IISER_Mohali--pic01.jpg)

## Dolor consequat feugiat amet veroeros

Feugiat dolor nullam orci pretium phasellus justo

### Mattis velit diam vulputate

Eget mattis at, laoreet vel et velit aliquam diam ante, aliquet sit amet vulputate et magna feugiat laoreet vel velit lorem.

### Lorem ipsum dolor sit veroeros

Eget mattis at, laoreet vel et velit aliquam diam ante, aliquet sit amet vulputate et magna feugiat laoreet vel velit lorem.

### Pretium phasellus justo lorem

Eget mattis at, laoreet vel et velit aliquam diam ante, aliquet sit amet vulputate et magna feugiat laoreet vel velit lorem.

### Tempus sed pretium orci

Eget mattis at, laoreet vel et velit aliquam diam ante, aliquet sit amet vulputate et magna feugiat laoreet vel velit lorem.

### Aliquam consequat et feugiat

Eget mattis at, laoreet vel et velit aliquam diam ante, aliquet sit amet vulputate et magna feugiat laoreet vel velit lorem.

### Dolore laoreet aliquam mattis

Eget mattis at, laoreet vel et velit aliquam diam ante, aliquet sit amet vulputate et magna feugiat laoreet vel velit lorem.

* Get Started
* More Info

The Endorsements

![]()

### Aliquam diam consequat

Eget mattis at, laoreet vel amet sed velit aliquam diam ante, dolor aliquet sit amet vulputate mattis amet laoreet lorem.

* Learn More

![]()

### Nisl adipiscing sed lorem

Eget mattis at, laoreet vel amet sed velit aliquam diam ante, dolor aliquet sit amet vulputate mattis amet laoreet lorem.

* Learn More

![]()

### Mattis tempus lorem

Eget mattis at, laoreet vel amet sed velit aliquam diam ante, dolor aliquet sit amet vulputate mattis amet laoreet lorem.

* Learn More

The Rest Of It

## Ipsum sapien elementum portitor?

Sed turpis tortor, tincidunt sed ornare in metus porttitor mollis nunc in aliquet.
Nam pharetra laoreet imperdiet volutpat etiam feugiat.

### Mailing Address

Untitled Corp
1234 Somewhere Rd
Nashville, TN 00000

### Social

@untitled-corp
linkedin.com/untitled
facebook.com/untitled

### Email

info@untitled.tld

### Phone

(000) 555-0000

* © Untitled.
* Design: [HTML5 UP](http://html5up.net)