iGEM 2017 — Team RPI_Troy_NY

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# Team:RPI Troy NY/Notebook

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MENU

[HOME](https://2017.igem.org/Team%3ARPI_Troy_NY)

TEAM

[Team](https://2017.igem.org/Team%3ARPI_Troy_NY/Team)
[Collaborations](https://2017.igem.org/Team%3ARPI_Troy_NY/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3ARPI_Troy_NY/Description)
[Design](https://2017.igem.org/Team%3ARPI_Troy_NY/Design)
[Experiments](https://2017.igem.org/Team%3ARPI_Troy_NY/Experiments)
[Notebook](https://2017.igem.org/Team%3ARPI_Troy_NY/Notebook)
[InterLab](https://2017.igem.org/Team%3ARPI_Troy_NY/InterLab)
[Contribution](https://2017.igem.org/Team%3ARPI_Troy_NY/Contribution)
[Model](https://2017.igem.org/Team%3ARPI_Troy_NY/Model)
[Results](https://2017.igem.org/Team%3ARPI_Troy_NY/Results)
[Demonstrate](https://2017.igem.org/Team%3ARPI_Troy_NY/Demonstrate)
[Improve](https://2017.igem.org/Team%3ARPI_Troy_NY/Improve)
[Attributions](https://2017.igem.org/Team%3ARPI_Troy_NY/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3ARPI_Troy_NY/Parts)
[Basic Parts](https://2017.igem.org/Team%3ARPI_Troy_NY/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3ARPI_Troy_NY/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3ARPI_Troy_NY/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3ARPI_Troy_NY/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3ARPI_Troy_NY/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3ARPI_Troy_NY/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3ARPI_Troy_NY/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3ARPI_Troy_NY/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3ARPI_Troy_NY/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3ARPI_Troy_NY/Hardware)
[Measurement](https://2017.igem.org/Team%3ARPI_Troy_NY/Measurement)
[Model](https://2017.igem.org/Team%3ARPI_Troy_NY/Model)
[Plant](https://2017.igem.org/Team%3ARPI_Troy_NY/Plant)
[Software](https://2017.igem.org/Team%3ARPI_Troy_NY/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=RPI_Troy_NY)

# RPI\_Troy\_NY

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

##### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

##### Inspiration

You can see what others teams have done to organize their notes:

* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)


[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:RPI Troy NY/Experiments

[![](http://placehold.it/350x150)](https://2017.igem.org/Team%3ARPI_Troy_NY)

MENU

[HOME](https://2017.igem.org/Team%3ARPI_Troy_NY)

TEAM

[Team](https://2017.igem.org/Team%3ARPI_Troy_NY/Team)
[Collaborations](https://2017.igem.org/Team%3ARPI_Troy_NY/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3ARPI_Troy_NY/Description)
[Design](https://2017.igem.org/Team%3ARPI_Troy_NY/Design)
[Experiments](https://2017.igem.org/Team%3ARPI_Troy_NY/Experiments)
[Notebook](https://2017.igem.org/Team%3ARPI_Troy_NY/Notebook)
[InterLab](https://2017.igem.org/Team%3ARPI_Troy_NY/InterLab)
[Contribution](https://2017.igem.org/Team%3ARPI_Troy_NY/Contribution)
[Model](https://2017.igem.org/Team%3ARPI_Troy_NY/Model)
[Results](https://2017.igem.org/Team%3ARPI_Troy_NY/Results)
[Demonstrate](https://2017.igem.org/Team%3ARPI_Troy_NY/Demonstrate)
[Improve](https://2017.igem.org/Team%3ARPI_Troy_NY/Improve)
[Attributions](https://2017.igem.org/Team%3ARPI_Troy_NY/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3ARPI_Troy_NY/Parts)
[Basic Parts](https://2017.igem.org/Team%3ARPI_Troy_NY/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3ARPI_Troy_NY/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3ARPI_Troy_NY/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3ARPI_Troy_NY/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3ARPI_Troy_NY/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3ARPI_Troy_NY/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3ARPI_Troy_NY/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3ARPI_Troy_NY/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3ARPI_Troy_NY/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3ARPI_Troy_NY/Hardware)
[Measurement](https://2017.igem.org/Team%3ARPI_Troy_NY/Measurement)
[Model](https://2017.igem.org/Team%3ARPI_Troy_NY/Model)
[Plant](https://2017.igem.org/Team%3ARPI_Troy_NY/Plant)
[Software](https://2017.igem.org/Team%3ARPI_Troy_NY/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=RPI_Troy_NY)

# RPI\_Troy\_NY

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

##### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

##### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

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[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:RPI Troy NY/Results

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|  |  |  |  |  |
| --- | --- | --- | --- | --- |
| [Home](https://2017.igem.org/Team%3ARPI_Troy_NY) | [Team](https://2017.igem.org/Team%3ARPI_Troy_NY/Team) | [Collaborations](https://2017.igem.org/Team%3ARPI_Troy_NY/Collaborations) | [Project](https://2017.igem.org/Team%3ARPI_Troy_NY/Description) | [Results](https://2017.igem.org/Team%3ARPI_Troy_NY/Results) |
| [Interlab Study](https://2017.igem.org/Team%3ARPI_Troy_NY/InterLab) | [Safety](https://2017.igem.org/Team%3ARPI_Troy_NY/Safety) | [Human Practices](https://2017.igem.org/Team%3ARPI_Troy_NY/HP/Silver) | [Attributions](https://2017.igem.org/Team%3ARPI_Troy_NY/Attributions) |

# Results

After culturing our control yeast strain, one with a full *MFE-2* gene knockout and one wild-type, it appeared there was not enough separation in the flasks to indicate enough sophorolipid yield. Unforeseen issues with primer design as well as fusion PCR delayed the microbial genetics committee by a few weeks. A new protocol was created by Dr. Gross and was implemented from November 3 through November 9. Final results were presented by the team at the Giant Jamboree.

### Preliminary Results

##### Below are previous Sophorolipid LCMS Data collected. This data will no longer be used. Final data to be presented at the Jamboree.

![](https://static.igem.org/mediawiki/2017/a/a6/Rpi_preliminary_results.png)

##### Below are the final data as presented at the Giant Jamboree. Our data demonstrate that selective modification of the *Starmerella bombicola* (yeast) *MFE-2* gene improves the efficiency of sophorolipid production.

![](https://static.igem.org/mediawiki/2017/6/67/Data_1.png)
![](https://static.igem.org/mediawiki/2017/e/ec/2017_Rpi_try_2.png)
![](https://static.igem.org/mediawiki/2017/e/ef/2017_rpi_troy_ny_data_3.png)
![](https://static.igem.org/mediawiki/2017/8/8f/2017_rpi_troy_ny_data_4.png)
![](https://static.igem.org/mediawiki/2017/7/76/2017_rpi_troy_ny_data_5.png)
![](https://static.igem.org/mediawiki/2017/a/a4/2017_rpi_troy_ny_data_6.png)

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