iGEM 2011 — Team Baltimore

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# Team:Baltimore/Notebook

### From 2011.igem.org

| [Home](/Team%3ABaltimore "Team:Baltimore") | [Team](/Team%3ABaltimore/Team "Team:Baltimore/Team") | [Project](/Team%3ABaltimore/Project "Team:Baltimore/Project") | [Parts Submitted to the Registry](/Team%3ABaltimore/Parts "Team:Baltimore/Parts") | [Modeling](/Team%3ABaltimore/Modeling "Team:Baltimore/Modeling") | **Notebook** | [Solutions](/Team%3ABaltimore/Solutions "Team:Baltimore/Solutions") | [Safety](/Team%3ABaltimore/Safety "Team:Baltimore/Safety") | [Attributions](/Team%3ABaltimore/Attributions "Team:Baltimore/Attributions") | [Hardware](/Team%3ABaltimore/Hardware "Team:Baltimore/Hardware") | [Material Safety Data Sheets](/wiki/index.php?title=Material_Safety_Data_Sheets&action=edit&redlink=1 "Material Safety Data Sheets (page does not exist)") | [http://partsregistry.org/assembly/libraries.cgi?id=35|MIT Parts Registry] |
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| Contents   * [1 Notebook](#Notebook)   + [1.1 Calendar](#Calendar)     - [1.1.1 July](#July)     - [1.1.2 August](#August)     - [1.1.3 September](#September) |

## Notebook

For more information about the project click [Dr. Tom's Notes](/wiki/index.php?title=Dr._Tom%27s_Notes&action=edit&redlink=1 "Dr. Tom's Notes (page does not exist)")

Run through for abstract and scheduling:

Start with plasmid with taq gene and pst1 site

Remove pst1 site from taq coding sequence

* In order to remove the restriction site, do site directed mutagenesis (1day)
* PCR
* Digestion
* [Transformation](/Team%3ABaltimore/Notebook/Transformation "Team:Baltimore/Notebook/Transformation")
* Screening (1day)
  + Dilute DNA
  + Colony PCR individually
  + Digestion of PCR product with pst1
  + Run gel ~2500bp

Add biobrick prefix/suffix to taq coding sequence

* PCR and add the prefix and suffix as primers (one prefix has an extra AG-make sure to use the correct one) (1-2 days)
  + Run gel
  + Cut out of gel
* Cut with restriction enzyme
* Clean up DNA
* Ligation with vector (could be vector with the terminator sequence, promoter and RBS)
* Transformation

[Add a promoter, transcriptional terminator, ribosome binding site (RBS)](/wiki/index.php?title=Team:Baltimore/Notebook/bb_assemblies&action=edit&redlink=1 "Team:Baltimore/Notebook/bb assemblies (page does not exist)")

* Screen colonies (1 day)
  + Colony PCR
  + Restriction Digestion
  + Clean up DNA
* Sequence (1 day)

Make taq protein

Compare it to other enzymes and make sure it works

### Calendar

---

#### July

**Week 1:** [Thursday, July 7](/wiki/index.php?title=Thursday,_July_7&action=edit&redlink=1 "Thursday, July 7 (page does not exist)");

**Week 2:** [Tuesday, July 12](/Tuesday%2C_July_12 "Tuesday, July 12"); [Wednesday, July 13](/Wednesday%2C_July_13 "Wednesday, July 13"); [Thursday, July 14](/Thursday%2C_July_14 "Thursday, July 14"); [Friday, July 15](/Friday%2C_July_15 "Friday, July 15"); [Saturday, July 16](/Saturday%2C_July_16 "Saturday, July 16"); [Sunday, July 17](/Sunday%2C_July_17 "Sunday, July 17");

**Week 3:** [Tuesday, July 19](/Tuesday%2C_July_19 "Tuesday, July 19"); [Wednesday, July 20](/Wednesday%2C_July_20 "Wednesday, July 20"); [Thursday, July 21](/Thursday%2C_July_21 "Thursday, July 21"); [Friday, July 22](/Friday%2C_July_22 "Friday, July 22"); [Saturday, July 23](/Saturday%2C_July_23 "Saturday, July 23"); [Sunday, July 24](/Sunday%2C_July_24 "Sunday, July 24");

**Week 4:** [Tuesday, July 26](/Tuesday%2C_July_26 "Tuesday, July 26"); [Wednesday, July 27](/Wednesday%2C_July_27 "Wednesday, July 27"); [Thursday, July 28](/Thursday%2C_July_28 "Thursday, July 28"); [Friday, July 29](/Friday%2C_July_29 "Friday, July 29"); [Saturday, July 30](/Saturday%2C_July_30 "Saturday, July 30"); [Sunday, July 31](/Sunday%2C_July_31 "Sunday, July 31");

#### August

**Week 1:** [Tuesday, August 2](/Tuesday%2C_August_2 "Tuesday, August 2"); [Wednesday, August 3](/Wednesday%2C_August_3 "Wednesday, August 3"); [Thursday, August 4](/Thursday%2C_August_4 "Thursday, August 4"); [Friday, August 5](/Friday%2C_August_5 "Friday, August 5"); [Saturday, August 6](/Saturday%2C_August_6 "Saturday, August 6"); [Sunday, August 7](/Sunday%2C_August_7 "Sunday, August 7");

**Week 2:** Week Off

**Week 3:** [Tuesday, August 16](/wiki/index.php?title=Tuesday,_August_16&action=edit&redlink=1 "Tuesday, August 16 (page does not exist)"); [Wednesday, August 17](/Wednesday%2C_August_17 "Wednesday, August 17"); [Thursday, August 18](/Thursday%2C_August_18 "Thursday, August 18"); [Friday, August 19](/wiki/index.php?title=Friday,_August_19&action=edit&redlink=1 "Friday, August 19 (page does not exist)"); [Saturday, August 20](/Saturday%2C_August_20 "Saturday, August 20"); [Sunday, August 21](/Sunday%2C_August_21 "Sunday, August 21");

Beginning in Week 4, we will be tightening up our iGEM ship for a multitude of reasons. READ the [New Rules](/New_Rules "New Rules").

**Week 4:** [Tuesday, August 23](/Tuesday%2C_August_23 "Tuesday, August 23"); [Wednesday, August 24](/Wednesday%2C_August_24 "Wednesday, August 24"); [Thursday, August 25](/Thursday%2C_August_25 "Thursday, August 25"); [Friday, August 26](/Friday%2C_August_26 "Friday, August 26"); [Saturday, August 27](/wiki/index.php?title=Saturday,_August_27&action=edit&redlink=1 "Saturday, August 27 (page does not exist)"); [Sunday, August 28](/Sunday%2C_August_28 "Sunday, August 28");

**Week 5:** [Tuesday, August 30](/Tuesday%2C_August_30 "Tuesday, August 30"); [Wednesday, August 31](/wiki/index.php?title=Wednesday,_August_31&action=edit&redlink=1 "Wednesday, August 31 (page does not exist)");

#### September

**Week 1:** [Thursday, September 1](/Thursday%2C_September_1 "Thursday, September 1"); [Friday, September 2](/Friday%2C_September_2 "Friday, September 2"); [Saturday, September 3](/wiki/index.php?title=Saturday,_September_3&action=edit&redlink=1 "Saturday, September 3 (page does not exist)"); [Sunday, September 4](/Sunday%2C_September_4 "Sunday, September 4");

**Week 2:** [Tuesday, September 6](/Tuesday%2C_September_6 "Tuesday, September 6"); [Thursday, September 8](/Thursday%2C_September_8 "Thursday, September 8"); [Friday, September 9](/Friday%2C_September_9 "Friday, September 9"); [Saturday, September 10](/Saturday%2C_September_10 "Saturday, September 10"); [Sunday, September 11](/Sunday%2C_September_11 "Sunday, September 11");

**Week 3:** [Tuesday, September 13](/Tuesday%2C_September_13 "Tuesday, September 13"); [Thursday, September 15](/Thursday%2C_September_15 "Thursday, September 15"); [Friday, September 16](/Friday%2C_September_16 "Friday, September 16"); [Saturday, September 17](/Saturday%2C_September_17 "Saturday, September 17"); [Sunday, September 18](/Sunday%2C_September_18 "Sunday, September 18");

**Week 4:** [Tuesday, September 20](/wiki/index.php?title=Tuesday,_September_20&action=edit&redlink=1 "Tuesday, September 20 (page does not exist)"); [Thursday, September 22](/Thursday%2C_September_22 "Thursday, September 22"); [Friday, September 23](/wiki/index.php?title=Friday,_September_23&action=edit&redlink=1 "Friday, September 23 (page does not exist)"); [Saturday, September 24](/wiki/index.php?title=Saturday,_September_24&action=edit&redlink=1 "Saturday, September 24 (page does not exist)"); [Sunday, September 25](/wiki/index.php?title=Sunday,_September_25&action=edit&redlink=1 "Sunday, September 25 (page does not exist)");

**Week 5:** [Tuesday, September 27](/wiki/index.php?title=Tuesday,_September_27&action=edit&redlink=1 "Tuesday, September 27 (page does not exist)"); [Wednesday, September 28](/wiki/index.php?title=Wednesday,_September_28&action=edit&redlink=1 "Wednesday, September 28 (page does not exist)");  [Thursday, September 29 - DEADLINE FOR EVERYTHING](/wiki/index.php?title=Thursday,_September_29_-_DEADLINE_FOR_EVERYTHING&action=edit&redlink=1 "Thursday, September 29 - DEADLINE FOR EVERYTHING (page does not exist)");

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