iGEM 2020 — Team SZPT-CHINA

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:SZPT-CHINA/Notebook

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:SZPT-CHINA/Engineering

[![logo](https://static.igem.org/mediawiki/2020/3/3c/T--SZPT-CHINA--header-logo.png)](https://2020.igem.org/Team%3ASZPT-CHINA)

* [Home](https://2020.igem.org/Team%3ASZPT-CHINA)
* Project
  + [Description](https://2020.igem.org/Team%3ASZPT-CHINA/Description)
  + [Design](https://2020.igem.org/Team%3ASZPT-CHINA/Design)
  + [Model](https://2020.igem.org/Team%3ASZPT-CHINA/Model)
  + [Note book](https://2020.igem.org/Team%3ASZPT-CHINA/Note%20book)
  + [Safety](https://2020.igem.org/Team%3ASZPT-CHINA/Safety)
* Experiment
  + [Protocols](https://2020.igem.org/Team%3ASZPT-CHINA/Protocols)
  + [Results](https://2020.igem.org/Team%3ASZPT-CHINA/Results)
  + [Future work](https://2020.igem.org/Team%3ASZPT-CHINA/Future%20work)
* Part
  + [Parts](https://2020.igem.org/Team%3ASZPT-CHINA/Parts)
  + [Contribution](https://2020.igem.org/Team%3ASZPT-CHINA/Contribution)
  + [Engineering](https://2020.igem.org/Team%3ASZPT-CHINA/Engineering)
  + [improve part](https://2020.igem.org/Team%3ASZPT-CHINA/Improvement%20part)
* Implementation
  + [Implementation](https://2020.igem.org/Team%3ASZPT-CHINA/Implementation)
  + [Proof Of Concept](https://2020.igem.org/Team%3ASZPT-CHINA/Proof%20Of%20Concept)
  + [Hardware](https://2020.igem.org/Team%3ASZPT-CHINA/Hardware)
  + [Entrepreneurship](https://2020.igem.org/Team%3ASZPT-CHINA/Entrepreneurship)
* Human Practices
  + [Integrated Human Practices](https://2020.igem.org/Team%3ASZPT-CHINA/Human%20Practices)
  + [Education](https://2020.igem.org/Team%3ASZPT-CHINA/Education)
* Team
  + [Members](https://2020.igem.org/Team%3ASZPT-CHINA/Team%20members)
  + [Attributions](https://2020.igem.org/Team%3ASZPT-CHINA/Attributions)
  + [Collaborations](https://2020.igem.org/Team%3ASZPT-CHINA/Collaborations)
  + [partnership](https://2020.igem.org/Team%3ASZPT-CHINA/Partnership)
* [Award](https://2020.igem.org/Team%3ASZPT-CHINA/Award)

# Engineering Success

Lysine riboswitch is regulated by lysine concentration.When the bacteria colonize the oral cavity, the riboswitch
controlled by lysine is turned off. When the bacteria escape the oral environment, the riboswitch opens and
expresses sfGFP.The results are shown in Fig.1. The bacteria are in the lysine at a concentration of 30μmol/mL.
Fluorescence expression was significantly inhibited, indicating that the lysine riboswitch can work normally.

![](https://static.igem.org/mediawiki/2020/0/0e/T--SZPT-CHINA--Experiment_Result_2020_22.png)

![](https://static.igem.org/mediawiki/2020/5/51/T--SZPT-CHINA--sponsors_2020_01.png)
![](https://static.igem.org/mediawiki/2020/f/fa/T--SZPT-CHINA--sponsors_2020_02.png)
![](https://static.igem.org/mediawiki/2020/8/8e/T--SZPT-CHINA--sponsors_2020_03.png)
![](https://static.igem.org/mediawiki/2020/3/35/T--SZPT-CHINA--sponsors_2020_04.png)
![](https://static.igem.org/mediawiki/2020/9/92/T--SZPT-CHINA--sponsors_2020_05.png)
![](https://static.igem.org/mediawiki/2020/5/50/T--SZPT-CHINA--sponsors_2020_06.png)
![](https://static.igem.org/mediawiki/2020/4/4a/T--SZPT-CHINA--sponsors_2020_07.png)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:SZPT-CHINA/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:SZPT-CHINA/Results

[![logo](https://static.igem.org/mediawiki/2020/3/3c/T--SZPT-CHINA--header-logo.png)](https://2020.igem.org/Team%3ASZPT-CHINA)

* [Home](https://2020.igem.org/Team%3ASZPT-CHINA)
* Project
  + [Description](https://2020.igem.org/Team%3ASZPT-CHINA/Description)
  + [Design](https://2020.igem.org/Team%3ASZPT-CHINA/Design)
  + [Model](https://2020.igem.org/Team%3ASZPT-CHINA/Model)
  + [Note book](https://2020.igem.org/Team%3ASZPT-CHINA/Note%20book)
  + [Safety](https://2020.igem.org/Team%3ASZPT-CHINA/Safety)
* Experiment
  + [Protocols](https://2020.igem.org/Team%3ASZPT-CHINA/Protocols)
  + [Results](https://2020.igem.org/Team%3ASZPT-CHINA/Results)
  + [Future work](https://2020.igem.org/Team%3ASZPT-CHINA/Future%20work)
* Part
  + [Parts](https://2020.igem.org/Team%3ASZPT-CHINA/Parts)
  + [Contribution](https://2020.igem.org/Team%3ASZPT-CHINA/Contribution)
  + [Engineering](https://2020.igem.org/Team%3ASZPT-CHINA/Engineering)
  + [improve part](https://2020.igem.org/Team%3ASZPT-CHINA/Improvement%20part)
* Implementation
  + [Implementation](https://2020.igem.org/Team%3ASZPT-CHINA/Implementation)
  + [Proof Of Concept](https://2020.igem.org/Team%3ASZPT-CHINA/Proof%20Of%20Concept)
  + [Hardware](https://2020.igem.org/Team%3ASZPT-CHINA/Hardware)
  + [Entrepreneurship](https://2020.igem.org/Team%3ASZPT-CHINA/Entrepreneurship)
* Human Practices
  + [Integrated Human Practices](https://2020.igem.org/Team%3ASZPT-CHINA/Human%20Practices)
  + [Education](https://2020.igem.org/Team%3ASZPT-CHINA/Education)
* Team
  + [Members](https://2020.igem.org/Team%3ASZPT-CHINA/Team%20members)
  + [Attributions](https://2020.igem.org/Team%3ASZPT-CHINA/Attributions)
  + [Collaborations](https://2020.igem.org/Team%3ASZPT-CHINA/Collaborations)
  + [partnership](https://2020.igem.org/Team%3ASZPT-CHINA/Partnership)
* [Award](https://2020.igem.org/Team%3ASZPT-CHINA/Award)

# Results

### Quick links:

## Contents

#### Detection system for *Streptococcus mutans*(*S.mutans*)

##### Prevention and treatment for dental caries

##### Suicide Mechanism

##### Part Ⅰ Prevention and treatment for dental caries

###### Screening result of antimicrobial peptides

Nine antimicrobial peptides were selected to inhibit the growth of *S.mutans*. The
minimum
inhibitory concentration
(MIC) results showed in table.1.

Table.1 MIC of nine antimicrobial peptides

![](https://static.igem.org/mediawiki/2020/4/4b/T--SZPT-CHINA--Experiment_Result_2020_01.png)

The smaller the MIC value, the lower concentration will be required to inhibit *S.mutans* growth. It shows that
the
inhibiting effect of antimicrobial peptide is better. According to Table.1, the MIC of TP-1 is 8μg/mL means that
these two antibacterial peptides have a high inhibit effect on *S.mutans*. TP-1 was selected to develop into
prevention and treatment products for dental caries.

###### Construction of the antimicrobial Peptides Multimer (AMPM)

Since TP-1 is composed of only a few amino acids, it is difficult to biosynthesize directly. Firstly, antimicrobial
Peptides Multimer was constructed. TP-1M containing 73 amino acids was joined by E, cleavage sites of Glu-C. The
amino acid sequence of TP-1M is shown in Fig.1.

![](https://static.igem.org/mediawiki/2020/9/95/T--SZPT-CHINA--Experiment_Result_2020_02.png)

Fig.1 Amino Acid Sequences of TP-1M

In order to further improve the inhibitory effect of TP-1 on *S.mutans*, we designed a specific targeting TP-1
sequence
(BTP-1) based on the signal molecule CSP in the quorum sensing signaling system of *S.mutans*. In order to
achieve the
expression of BTP-1, we used E as the connection site to form a specific targeting antimicrobial peptide polymer
(BTP-1M). The amino acid sequence of BTP-1M is shown in Fig.2 below.

![](https://static.igem.org/mediawiki/2020/a/a8/T--SZPT-CHINA--Experiment_Result_2020_03.png)

![](https://static.igem.org/mediawiki/2020/5/51/T--SZPT-CHINA--sponsors_2020_01.png)
![](https://static.igem.org/mediawiki/2020/f/fa/T--SZPT-CHINA--sponsors_2020_02.png)
![](https://static.igem.org/mediawiki/2020/8/8e/T--SZPT-CHINA--sponsors_2020_03.png)
![](https://static.igem.org/mediawiki/2020/3/35/T--SZPT-CHINA--sponsors_2020_04.png)
![](https://static.igem.org/mediawiki/2020/9/92/T--SZPT-CHINA--sponsors_2020_05.png)
![](https://static.igem.org/mediawiki/2020/5/50/T--SZPT-CHINA--sponsors_2020_06.png)
![](https://static.igem.org/mediawiki/2020/4/4a/T--SZPT-CHINA--sponsors_2020_07.png)