iGEM 2010 — Team IvyTech-South_Bend

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# Team:IvyTech-South Bend/Notebook

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 [![](https://static.igem.org/mediawiki/2010/8/84/Safety.png)](https://2010.igem.org/Team%3AIvyTech-South_Bend/Safety)
 [![](https://static.igem.org/mediawiki/2010/f/f7/Plan.png)](https://2010.igem.org/Talk%3ATeam%3AIvyTech-South_Bend)
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[Discussion](https://2010.igem.org/Talk%3ATeam%3AIvyTech-South_Bend/Notebook)

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| Contents   * [1 Notebook](#Notebook)   + [1.1 IGEM Part #s and Uses](#IGEM_Part_.23s_and_Uses) * [2 6/28/10](#6.2F28.2F10) * [3 6/30/10](#6.2F30.2F10) * [4 7/28/10](#7.2F28.2F10) * [5 8/24/10](#8.2F24.2F10) * [6 8/27/10](#8.2F27.2F10) * [7 8/27/10](#8.2F27.2F10_2) * [8 8/31/10](#8.2F31.2F10) * [9 8/31/10](#8.2F31.2F10_2) * [10 9/1/10](#9.2F1.2F10) * [11 9/2/10](#9.2F2.2F10) * [12 9/2/10](#9.2F2.2F10_2) * [13 9/3/10](#9.2F3.2F10) * [14 9/9/10](#9.2F9.2F10) * [15 9/10/10](#9.2F10.2F10) * [16 9/14/10](#9.2F14.2F10) * [17 9/15/10](#9.2F15.2F10) * [18 9/16/10](#9.2F16.2F10) * [19 9/17/10](#9.2F17.2F10) * [20 10/18/10](#10.2F18.2F10) * [21 9/21/10](#9.2F21.2F10) * [22 9/22/10](#9.2F22.2F10) * [23 9/22/10](#9.2F22.2F10_2) * [24 9/23/10](#9.2F23.2F10) * [25 9/24/10](#9.2F24.2F10) * [26 9/28/10](#9.2F28.2F10) * [27 9/30/10](#9.2F30.2F10) * [28 10/1/10](#10.2F1.2F10) * [29 10/5/10](#10.2F5.2F10) * [30 10/7/10](#10.2F7.2F10) * [31 10/8/10](#10.2F8.2F10) * [32 10/12/10](#10.2F12.2F10) * [33 10/13/10](#10.2F13.2F10) * [34 10/15/10](#10.2F15.2F10) * [35 10/19/10](#10.2F19.2F10) * [36 10/21/10](#10.2F21.2F10) * [37 10/25/10](#10.2F25.2F10) * [38 10/26/10](#10.2F26.2F10) |

## Notebook

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| |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | June | | | | | | | | M | T | W | T | F | S | S | |  | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/1\_June\_2010&action=edit 1] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/2\_June\_2010&action=edit 2] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/3\_June\_2010&action=edit 3] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/4\_June\_2010&action=edit 4] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/5\_June\_2010&action=edit 5] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/6\_June\_2010&action=edit 6] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/7\_June\_2010&action=edit 7] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/8\_June\_2010&action=edit 8] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/9\_June\_2010&action=edit 9] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/10\_June\_2010&action=edit 10] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/11\_June\_2010&action=edit 11] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/12\_June\_2010&action=edit 12] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/13\_June\_2010&action=edit 13] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/14\_June\_2010&action=edit 14] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/15\_June\_2010&action=edit 15] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/16\_June\_2010&action=edit 16] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/17\_June\_2010&action=edit 17] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/18\_June\_2010&action=edit 18] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/19\_June\_2010&action=edit 19] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/20\_June\_2010&action=edit 20] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/21\_June\_2010&action=edit 21] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/22\_June\_2010&action=edit 22] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/23\_June\_2010&action=edit 23] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/24\_June\_2010&action=edit 24] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/25\_June\_2010&action=edit 25] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/26\_June\_2010&action=edit 26] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/27\_June\_2010&action=edit 27] | | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/28\_June\_2010 28] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/29\_June\_2010 29] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/30\_June\_2010 30] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | July | | | | | | | | M | T | W | T | F | S | S | |  |  |  | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/1\_July\_2010 1] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/2\_July\_2010&action=edit 2] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/3\_July\_2010&action=edit 3] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/4\_July\_2010&action=edit 4] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/5\_July\_2010&action=edit 5] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/6\_July\_2010&action=edit 6] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/7\_July\_2010&action=edit 7] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/8\_July\_2010&action=edit 8] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/9\_July\_2010&action=edit 9] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/10\_July\_2010&action=edit 10] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/11\_July\_2010&action=edit 11] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/12\_July\_2010&action=edit 12] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/13\_July\_2010&action=edit 13] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/14\_July\_2010&action=edit 14] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/15\_July\_2010&action=edit 15] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/16\_July\_2010&action=edit 16] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/17\_July\_2010&action=edit 17] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/18\_July\_2010&action=edit 18] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/19\_July\_2010&action=edit 19] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/20\_July\_2010&action=edit 20] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/21\_July\_2010&action=edit 21] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/22\_July\_2010&action=edit 22] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/23\_July\_2010&action=edit 23] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/24\_July\_2010&action=edit 24] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/25\_July\_2010&action=edit 25] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/26\_July\_2010&action=edit 26] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/27\_July\_2010&action=edit 27] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/28\_July\_2010 28] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/29\_July\_2010&action=edit 29] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/30\_July\_2010&action=edit 30] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/31\_July\_2010&action=edit 31] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | August | | | | | | | | M | T | W | T | F | S | S | |  |  |  |  |  |  | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/1\_August\_2010&action=edit 1] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/2\_August\_2010&action=edit 2] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/3\_August\_2010&action=edit 3] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/4\_August\_2010&action=edit 4] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/5\_August\_2010&action=edit 5] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/6\_August\_2010&action=edit 6] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/7\_August\_2010&action=edit 7] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/8\_August\_2010&action=edit 8] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/9\_August\_2010&action=edit 9] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/10\_August\_2010&action=edit 10] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/11\_August\_2010&action=edit 11] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/12\_August\_2010&action=edit 12] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/13\_August\_2010&action=edit 13] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/14\_August\_2010&action=edit 14] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/15\_August\_2010&action=edit 15] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/16\_August\_2010&action=edit 16] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/17\_August\_2010&action=edit 17] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/18\_August\_2010&action=edit 18] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/19\_August\_2010&action=edit 19] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/20\_August\_2010&action=edit 20] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/21\_August\_2010&action=edit 21] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/22\_August\_2010&action=edit 22] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/23\_August\_2010&action=edit 23] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/24\_August\_2010 24] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/25\_August\_2010&action=edit 25] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/26\_August\_2010&action=edit 26] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/27\_August\_2010 27] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/28\_August\_2010&action=edit 28] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/29\_August\_2010&action=edit 29] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/30\_August\_2010&action=edit 30] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/31\_August\_2010 31] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | September | | | | | | | | M | T | W | T | F | S | S | |  |  | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/1\_September\_2010 1] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/2\_September\_2010 2] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/3\_September\_2010 3] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/4\_September\_2010&action=edit 4] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/5\_September\_2010&action=edit 5] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/6\_September\_2010&action=edit 6] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/7\_September\_2010&action=edit 7] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/8\_September\_2010&action=edit 8] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/9\_September\_2010 9] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/10\_September\_2010 10] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/11\_September\_2010&action=edit 11] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/12\_September\_2010&action=edit 12] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/13\_September\_2010&action=edit 13] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/14\_September\_2010 14] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/15\_September\_2010 15] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/16\_September\_2010 16] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/17\_September\_2010 17] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/18\_September\_2010&action=edit 18] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/19\_September\_2010&action=edit 19] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/20\_September\_2010&action=edit 20] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/21\_September\_2010 21] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/22\_September\_2010 22] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/23\_September\_2010 23] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/24\_September\_2010 24] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/25\_September\_2010&action=edit 25] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/26\_September\_2010&action=edit 26] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/27\_September\_2010&action=edit 27] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/28\_September\_2010 28] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/29\_September\_2010&action=edit 29] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/30\_September\_2010 30] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | October | | | | | | | | M | T | W | T | F | S | S | |  |  |  |  | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/1\_October\_2010 1] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/2\_October\_2010&action=edit 2] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/3\_October\_2010&action=edit 3] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/4\_October\_2010&action=edit 4] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/5\_October\_2010 5] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/6\_October\_2010 6] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/7\_October\_2010 7] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/8\_October\_2010 8] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/9\_October\_2010&action=edit 9] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/10\_October\_2010&action=edit 10] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/11\_October\_2010&action=edit 11] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/12\_October\_2010 12] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/13\_October\_2010 13] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/14\_October\_2010 14] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/15\_October\_2010 15] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/16\_October\_2010&action=edit 16] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/17\_October\_2010&action=edit 17] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/18\_October\_2010&action=edit 18] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/19\_October\_2010 19] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/20\_October\_2010 20] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/21\_October\_2010 21] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/22\_October\_2010 22] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/23\_October\_2010 23] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/24\_October\_2010 24] | | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/25\_October\_2010 25] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/26\_October\_2010 26] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/27\_October\_2010&action=edit 27] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/28\_October\_2010&action=edit 28] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/29\_October\_2010&action=edit 29] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/30\_October\_2010&action=edit 30] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/31\_October\_2010&action=edit 31] | | |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | | November | | | | | | | | M | T | W | T | F | S | S | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/1\_November\_2010&action=edit 1] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/2\_November\_2010&action=edit 2] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/3\_November\_2010&action=edit 3] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/4\_November\_2010&action=edit 4] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/5\_November\_2010&action=edit 5] | [http://2010.igem.org/Talk:Team:IvyTech-South\_Bend/6\_November\_2010 6] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/7\_November\_2010&action=edit 7] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/8\_November\_2010&action=edit 8] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/9\_November\_2010&action=edit 9] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/10\_November\_2010&action=edit 10] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/11\_November\_2010&action=edit 11] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/12\_November\_2010&action=edit 12] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/13\_November\_2010&action=edit 13] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/14\_November\_2010&action=edit 14] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/15\_November\_2010&action=edit 15] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/16\_November\_2010&action=edit 16] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/17\_November\_2010&action=edit 17] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/18\_November\_2010&action=edit 18] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/19\_November\_2010&action=edit 19] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/20\_November\_2010&action=edit 20] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/21\_November\_2010&action=edit 21] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/22\_November\_2010&action=edit 22] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/23\_November\_2010&action=edit 23] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/24\_November\_2010&action=edit 24] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/25\_November\_2010&action=edit 25] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/26\_November\_2010&action=edit 26] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/27\_November\_2010&action=edit 27] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/28\_November\_2010&action=edit 28] | | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/29\_November\_2010&action=edit 29] | [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South\_Bend/30\_November\_2010&action=edit 30] | |

### IGEM Part #s and Uses

BBa\_T9002 – Producer Controler Device–Plate2 Well 9A

BBa\_I732017 – LacZ with our GFP 1—Plate 2 Well 3K

BBa\_F2620– LuxR with terminator– Plate 2 Well 6E

BBa\_I13522 –Tet Prop with GFP—Plate 2 Well 8A

BBa\_T13521 Red Flouro tet RFP—Plate 2 Well 6O

BBa\_pSB1C3—Plasmid—Plate 1 Well 3A

BBa\_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A

BBa\_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A

## 6/28/10

Preparing SOB

## 6/30/10

Richard Lab:Electroporation of E. coli

## 7/28/10

Usage and extraction

## 8/24/10

Today we will be pouring new plates for streaking new transformed cells.

## 8/27/10

We will be running a gel to determine if our DNA sample was successfully electroplated.

## 8/27/10

Today we will be electroplating part KBBa\_3131010

## 8/31/10

Results of transformation - the plates had growth but (no) distinct colony pattern.

## 8/31/10

After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH

## 9/1/10

Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.

## 9/2/10

Results from streaking –

## 9/2/10

Protocol For Making LB-Broth/Agar

## 9/3/10

Today I’m finishing making the LB/Agar from yesterday.

## 9/9/10

Due to conflict we will be changing from E Coli to yeast.

## 9/10/10

Lux Casette Right Promoter BBa\_I1051

## 9/14/10

Today we found growth from our Electroporated E – Coli parts

## 9/15/10

Today we will be extracting DNA from out electroporated cells/T9002
B-galactosidase Assay

## 9/16/10

Today we will wash

## 9/17/10

Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!

## 10/18/10

cutting enzymes

## 9/21/10

Today we will electroporate Agrobacterium with part T9002

Electrophoresis

## 9/22/10

Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample

## 9/22/10

The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens

## 9/23/10

Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.

## 9/24/10

Today I’m testing absorbance of our trials @ 395nm

## 9/28/10

Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10

## 9/30/10

LacZ without GFP also with RBS and terminator

## 10/1/10

electrophoresis

## 10/5/10

pTet GFP

## 10/7/10

Electroporating 2 parts with E-coli bacteria

## 10/8/10

LB-Lennox Broth & Electroporation

## 10/12/10

hold until Friday

## 10/13/10

Discoveries!

## 10/15/10

Using openwetware.org protocol

B-galactosidase Assay

BBL Trypticase soy Broth and Agar

DPBS

## 10/19/10

Protocol-- Electroporation man for E-Coli

## 10/21/10

High Efficiency Electrotransformation of E.coli

## 10/25/10

DNA from BlueGuy

## 10/26/10

Final steps pack and ship

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