iGEM 2017 — Team Fudan

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# Team:Fudan/Notebook

* ![](https://static.igem.org/mediawiki/2017/9/97/T--Fudan--logo-dark-sm.png)
* [HOME](https://2017.igem.org/Team%3AFudan)
* [Project](https://2017.igem.org/Team%3AFudan/Project)
  + [SwordS](https://2017.igem.org/Team%3AFudan/Applied_Design)
  + [HCC](https://2017.igem.org/Team%3AFudan/HCC)
* [Results](https://2017.igem.org/Team%3AFudan/Results)
  + [Demonstrate](https://2017.igem.org/Team%3AFudan/Demonstrate)
  + [Basic Part](https://2017.igem.org/Team%3AFudan/Basic_Part)
  + [Composite Part](https://2017.igem.org/Team%3AFudan/Composite_Part)
  + [Part Collection](https://2017.igem.org/Team%3AFudan/Part_Collection)
* [Lab](https://2017.igem.org/Team%3AFudan/Lab)
  + [Safety](https://2017.igem.org/Team%3AFudan/Safety)
  + [InterLab](https://2017.igem.org/Team%3AFudan/InterLab)
  + [Notebook](https://2017.igem.org/Team%3AFudan/Notebook)
* [Model](https://2017.igem.org/Team%3AFudan/ModelandSoftware)
  + [Model](https://2017.igem.org/Team%3AFudan/Model)
  + [Software](https://2017.igem.org/Team%3AFudan/Software)
* [HP](https://2017.igem.org/Team%3AFudan/HP)
  + [Silver HP](https://2017.igem.org/Team%3AFudan/HP/Silver)
  + [Integrated HP](https://2017.igem.org/Team%3AFudan/HP/Gold_Integrated)
  + [Cell War!](https://2017.igem.org/Team%3AFudan/Engagement)
  + [Collaboration](https://2017.igem.org/Team%3AFudan/Collaborations)
* [Team](https://2017.igem.org/Team%3AFudan/Team)
  + [Teamers](https://2017.igem.org/Team%3AFudan/Teamers)
  + [Acknowledgement](https://2017.igem.org/Team%3AFudan/Attributions#Acknowledgement)
  + [Attributions](https://2017.igem.org/Team%3AFudan/Attributions#Attributions)

| [![](https://static.igem.org/mediawiki/2017/8/87/T--Fudan--safety.png)](https://2017.igem.org/Team%3AFudan/Safety) | [![](https://static.igem.org/mediawiki/2017/0/03/T--Fudan--interlab.png)](https://2017.igem.org/Team%3AFudan/InterLab) | [![](https://static.igem.org/mediawiki/2017/3/35/T--Fudan--notebook.png)](https://2017.igem.org/Team%3AFudan/Notebook) |

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# Team:Fudan/Experiments

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MENU

[HOME](https://2017.igem.org/Team%3AFudan)

TEAM

[Team](https://2017.igem.org/Team%3AFudan/Team)
[Collaborations](https://2017.igem.org/Team%3AFudan/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3AFudan/Description)
[Design](https://2017.igem.org/Team%3AFudan/Design)
[Experiments](https://2017.igem.org/Team%3AFudan/Experiments)
[Notebook](https://2017.igem.org/Team%3AFudan/Notebook)
[InterLab](https://2017.igem.org/Team%3AFudan/InterLab)
[Contribution](https://2017.igem.org/Team%3AFudan/Contribution)
[Model](https://2017.igem.org/Team%3AFudan/Model)
[Results](https://2017.igem.org/Team%3AFudan/Results)
[Demonstrate](https://2017.igem.org/Team%3AFudan/Demonstrate)
[Improve](https://2017.igem.org/Team%3AFudan/Improve)
[Attributions](https://2017.igem.org/Team%3AFudan/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3AFudan/Parts)
[Basic Parts](https://2017.igem.org/Team%3AFudan/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3AFudan/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3AFudan/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3AFudan/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3AFudan/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3AFudan/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3AFudan/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3AFudan/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3AFudan/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3AFudan/Hardware)
[Measurement](https://2017.igem.org/Team%3AFudan/Measurement)
[Model](https://2017.igem.org/Team%3AFudan/Model)
[Plant](https://2017.igem.org/Team%3AFudan/Plant)
[Software](https://2017.igem.org/Team%3AFudan/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=Fudan)

# Fudan

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

##### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

##### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

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# Team:Fudan/Results

* ![](https://static.igem.org/mediawiki/2017/9/97/T--Fudan--logo-dark-sm.png)
* [HOME](https://2017.igem.org/Team%3AFudan)
* [Project](https://2017.igem.org/Team%3AFudan/Project)
  + [SwordS](https://2017.igem.org/Team%3AFudan/Applied_Design)
  + [HCC](https://2017.igem.org/Team%3AFudan/HCC)
* [Results](https://2017.igem.org/Team%3AFudan/Results)
  + [Demonstrate](https://2017.igem.org/Team%3AFudan/Demonstrate)
  + [Basic Part](https://2017.igem.org/Team%3AFudan/Basic_Part)
  + [Composite Part](https://2017.igem.org/Team%3AFudan/Composite_Part)
  + [Part Collection](https://2017.igem.org/Team%3AFudan/Part_Collection)
* [Lab](https://2017.igem.org/Team%3AFudan/Lab)
  + [Safety](https://2017.igem.org/Team%3AFudan/Safety)
  + [InterLab](https://2017.igem.org/Team%3AFudan/InterLab)
  + [Notebook](https://2017.igem.org/Team%3AFudan/Notebook)
* [Model](https://2017.igem.org/Team%3AFudan/ModelandSoftware)
  + [Model](https://2017.igem.org/Team%3AFudan/Model)
  + [Software](https://2017.igem.org/Team%3AFudan/Software)
* [HP](https://2017.igem.org/Team%3AFudan/HP)
  + [Silver HP](https://2017.igem.org/Team%3AFudan/HP/Silver)
  + [Integrated HP](https://2017.igem.org/Team%3AFudan/HP/Gold_Integrated)
  + [Cell War!](https://2017.igem.org/Team%3AFudan/Engagement)
  + [Collaboration](https://2017.igem.org/Team%3AFudan/Collaborations)
* [Team](https://2017.igem.org/Team%3AFudan/Team)
  + [Teamers](https://2017.igem.org/Team%3AFudan/Teamers)
  + [Acknowledgement](https://2017.igem.org/Team%3AFudan/Attributions#Acknowledgement)
  + [Attributions](https://2017.igem.org/Team%3AFudan/Attributions#Attributions)

# Results

Antigen density on tumor cells’ surface is heterogeneous. Current cellular immunotherapy only targets cells with high expression of specific tumor antigen. While this approach can improve the precision of recognition, it loses the opportunity to strategically treat tumor cells with different surface antigen densities. We are the first to propose a cellular immunotherapy platform, SwordS (SynNotch-Stripe system), that is capable of generating non-monotonic therapeutic responses to one tumor antigen with different surface densities. We demonstrated our concept with the following experiments. In combining a SynNotch module, which can recognize the antigen, and a Stripe module, which can sort intracellular signal, our engineered cells could generate antigen density dependent tri-response against the target cells with different densities of surface GFP, as well as membrane GPC3 (the tumor antigen of hepatocellular carcinoma). Both experimental data and mathematical simulation show that our platform not only reduces the on-target/off-tumor effects of the original SynNotch system, but also provides a combinational therapeutic solution to treat tumor at various states. We believe that SwordS is a promising platform for the next generation cellular immunotherapy.

| [![](https://static.igem.org/mediawiki/2017/9/93/T--Fudan--synNotch.png)](https://2017.igem.org/Team%3AFudan/Demonstrate) | [![](https://static.igem.org/mediawiki/2017/2/24/T--Fudan--hccOrange.png)](https://2017.igem.org/Team%3AFudan/Basic_Part) | [![](https://static.igem.org/mediawiki/2017/6/66/T--Fudan--synTF.png)](https://2017.igem.org/Team%3AFudan/Composite_Part) | [![](https://static.igem.org/mediawiki/2017/0/0c/T--Fudan--stripe.png)](https://2017.igem.org/Team%3AFudan/Part_Collection) |

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