iGEM 2008 — Team iHKU

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# Team:iHKU/Notebook

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## Wet lab log book for the biobrick subgroup

(Many Thanks to MIT team for setting a good example for us ^\_^)

* Click on any day below to see what wet-lab procedures were conducted.

* log book summary

0703 done: punched: and transformed. BBa\_Q04510 BBa\_R0062 BBa\_R0011 BBa\_I732096

## Planning and summary section for the biobrick subgroup

here is the Biobrick subgroup progress,

* Roy has got these following biobircks:

[http://partsregistry.org/Part:BBa\_E0240 Part:BBa\_E0240] Colony standard measurement for promotor

p1010 psb3K3

* parts already obtained by peter：

[http://partsregistry.org/Part:BBa\_F2622 Part:BBa\_F2622] ***Colony***

[http://partsregistry.org/Part:BBa\_T9002 Part:BBa\_T9002] ***Punch***

[http://partsregistry.org/Part:BBa\_I731007 Part:BBa\_I731007] ***Punch***

[http://partsregistry.org/Part:BBa\_B0030 Part:BBa\_B0030] ***Punch***

S17 strain colony ready for ccdB

* parts we further need to get

[http://partsregistry.org/Part:BBa\_Q04510 Part:BBa\_Q04510] 1013 7A ***Transf***

[http://partsregistry.org/Part:BBa\_R0062 Part:BBa\_R0062] 1000 4G ***Transf***

[http://partsregistry.org/Part:BBa\_R0011 Part:BBa\_R0011] 1000 4b ***Transf***

[http://partsregistry.org/Part:BBa\_I732096 BBa\_I732096] 1014 3G ***Transf***
reporter containing the standard GFP and LacZa

* parts that may be useful

http://partsregistry.org/Part:BBa\_B0031

http://partsregistry.org/Part:BBa\_B0032

http://partsregistry.org/Part:BBa\_B0033

http://partsregistry.org/Part:BBa\_B0034

* parts that we need to build:

1 [http://partsregistry.org/Part:BBa\_K094001 Part:BBa\_K094001]CheZ assumed without rbs

2 [http://partsregistry.org/Part:BBa\_K094002 Part:BBa\_K094002]LuxRI assumed with rbs and terminator

* composite we need to construct:

1[http://partsregistry.org/Part:BBa\_K094003 plaLuxRpluxCheZGFPLva] BBa\_F2622 BBa\_B0030 BBa\_K094001 BBa\_B0030 BBa\_E0022 BBa\_B0015
（something wrong with GFPLva and i will change them later）

2[http://partsregistry.org/Part:BBa\_K094004 plaLuxRpluxCIpldCheZGFPLva] BBa\_F2622 BBa\_Q04510 BBa\_B0030 BBa\_K094001 BBa\_E0430

3[http://partsregistry.org/Part:BBa\_K094005 plaLuxRpluxLacZ] BBa\_F2622 BBa\_B0030 BBa\_I714901 BBa\_B0015

4[http://partsregistry.org/Part:BBa\_K094006 plaLuxRpluxCIpldLacZ] BBa\_F2622 BBa\_Q04510 BBa\_B0030 BBa\_I714901 BBa\_B0015

5[http://partsregistry.org/Part:BBa\_K094007 plaLuxRIpluxCheZGFPLva] BBa\_R0011 BBa\_K094002 BBa\_R0062 BBa\_B0030 BBa\_K094001 BBa\_E0430

6 [http://partsregistry.org/Part:BBa\_K094008 first]

7 [http://partsregistry.org/Part:BBa\_K094009 Part:BBa\_K094009]

parts entered on 080822

[http://partsregistry.org/Part:BBa\_K094011 11]

[http://partsregistry.org/Part:BBa\_K094012 12]

[http://partsregistry.org/Part:BBa\_K094013 13]

[http://partsregistry.org/Part:BBa\_K094014 14]

[http://partsregistry.org/Part:BBa\_K094015 15]

[http://partsregistry.org/Part:BBa\_K094016 16]

[http://partsregistry.org/Part:BBa\_K094017 17]

[http://partsregistry.org/Part:BBa\_K094018 18]

## Notebook

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