iGEM 2013 — Team Tokyo_Tech

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# Team:Tokyo Tech/Notebook

### From 2013.igem.org

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* [Home](https://2013.igem.org/Team%3ATokyo_Tech)
* Project
  + [Ninja State Switching](https://2013.igem.org/Team%3ATokyo_Tech/Project/Ninja_State_Switching)
  + [M13 "Shuriken"](https://2013.igem.org/Team%3ATokyo_Tech/Project/M13_Shuriken)
  + [Farming](https://2013.igem.org/Team%3ATokyo_Tech/Project/Farming)
* Experiment
  + [Crosstalk Confirmation Assay](https://2013.igem.org/Team%3ATokyo_Tech/Experiment/Crosstalk_Confirmation_Assay)
  + [Crosstalk Circumvention Assay](https://2013.igem.org/Team%3ATokyo_Tech/Experiment/Crosstalk_Circumvention_Assay)
  + [pSB-M13 Plasmid Assay](https://2013.igem.org/Team%3ATokyo_Tech/Experiment/pSB-M13_Plasmid_Assay)
  + [Inducible Plaque Forming Assay](https://2013.igem.org/Team%3ATokyo_Tech/Experiment/Inducible_Plaque_Forming_Assay)
  + [*phoA* Promoter Assay](https://2013.igem.org/Team%3ATokyo_Tech/Experiment/phoA_Promoter_Assay)
  + [Quantitative Analysis for Cytokinin](https://2013.igem.org/Team%3ATokyo_Tech/Experiment/Quantitative_Analysis_of_Cytokinin)
  + [*RM/lac* Hybrid Promoter Assay](https://2013.igem.org/Team%3ATokyo_Tech/Experiment/RM-lac_Hybrid_Promoter_Assay)
* Modeling
  + [Crosstalk Circumvention](https://2013.igem.org/Team%3ATokyo_Tech/Modeling/Crosstalk_Circumvention)
  + [Incoherent Feed Forward Loop](https://2013.igem.org/Team%3ATokyo_Tech/Modeling/Incoherent_Feed_Forward_Loop)
* [Submitted Parts](https://2013.igem.org/Team%3ATokyo_Tech/Submitted_Parts)
* [Human Practice](https://2013.igem.org/Team%3ATokyo_Tech/Human_Practice)
* More
  + [Safety](https://2013.igem.org/Team%3ATokyo_Tech/More/Safety)
  + [Attribution and Contribution](https://2013.igem.org/Team%3ATokyo_Tech/More/Attribution_and_Contribution)
  + [Team](https://2013.igem.org/Team%3ATokyo_Tech/More/Team)
  + [Collaboration](https://2013.igem.org/Team%3ATokyo_Tech/More/Collaboration)
  + [Sponsors](https://2013.igem.org/Team%3ATokyo_Tech/More/Sponsors)
  + [Origami](https://2013.igem.org/Team%3ATokyo_Tech/More/Origami)
  + [Official Team Profile](https://igem.org/Team.cgi?id=1139)
  + [Judging form](https://2013.igem.org/Team%3ATokyo_Tech/More/Judging_form)

# Overview

## A love story contains several processes. Two people fall in love and their love burning wildly. However, no forever exists in the world, in most occasions, love will eventually burn to only a pile of ashes of the last remaining wind drift away. In our project, we have recreated the story of "Romeo and Juliet" by Shakespeare vividly by two kinds of Escherichia coli cell-cell communication. We aim to generate a circuit involving regulatory mechanism of positive feedback rather than commonly-used negative feedback to control the fate of E.coli by signaling between two types of E.coli. Besides, Rose represents love. We are the first iGEM group ever to synthesize P(3HB) (a kind of bio-plastics) by using our new biobrick part, representing rose . Ⅰ cell-cell communication "Romeo and Juliet" is the drama by dramatist William Shakespeare of England. The stage is city Verona, Italy in the 14th century. Romeo met Juliet. Two people fell in love instantly. In this project, we will recreate the love story of "Romeo and Juliet".