iGEM 2010 — Team CBNU-Korea

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# Team:CBNU-Korea/Notebook

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5/7

8/25
I51020 transformation failed
Plan to design Cn vector
Ready to P1003, *ori* miniprep

9/2

Primer design for Cn vector. May be it won’t work….

All parts re-transformation for selection and miniprep.

9/7

Cn vector primer arrived.
Ready to PSB1C3 plasmid miniprep
*Ori* X/S digestion**.**

**9/10**

**Cn vector PCR, purification, ligation.**
**Cn vector PCR Product electroporhesis.**
**9/15**
**Cn vector transformation failed.**
**Cn vercor PCR condition re arrangement**
**PrctB promoter primer design**

**9/25**
**Cn vector + *or*i(X-S) ligation, transformation.**
**PrctB primer arrived , PCR**

**9/27**
**Cn vector colony appear. But not red, may be failure.**
**PrctB + rctB ligation.**
**Cn vector transformation**

**9/30**
**Cn vector colony appear again. Not red too. Ready to miniprep.**
**parB + GFP fusion**

**10/3**
**parA, parS, dif ligation PSB1C3 and PSB1A3**
**computer engineering student meeting,**

**10/8**
**Ori PCR and miniprep**
**Ori digestion EP/XP/XS**

**10/10**

**parS, dif parts complete.**
**parA re PCR.**

**10/15**
**All members of CBNU-KOREA team meeting**

**10/21**
**Parts making start.**

**Ori function is not checked.**

**10/25**

**Parts sending**
**Prepare Team wiki freeze**

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