iGEM 2017 — Team TU_Dresden

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# Team:TU Dresden/Notebook

[![](http://placehold.it/350x150)](https://2017.igem.org/Team%3ATU_Dresden)

MENU

[HOME](https://2017.igem.org/Team%3ATU_Dresden)

TEAM

[Team](https://2017.igem.org/Team%3ATU_Dresden/Team)
[Collaborations](https://2017.igem.org/Team%3ATU_Dresden/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3ATU_Dresden/Description)
[Design](https://2017.igem.org/Team%3ATU_Dresden/Design)
[Experiments](https://2017.igem.org/Team%3ATU_Dresden/Experiments)
[Notebook](https://2017.igem.org/Team%3ATU_Dresden/Notebook)
[InterLab](https://2017.igem.org/Team%3ATU_Dresden/InterLab)
[Contribution](https://2017.igem.org/Team%3ATU_Dresden/Contribution)
[Model](https://2017.igem.org/Team%3ATU_Dresden/Model)
[Results](https://2017.igem.org/Team%3ATU_Dresden/Results)
[Demonstrate](https://2017.igem.org/Team%3ATU_Dresden/Demonstrate)
[Improve](https://2017.igem.org/Team%3ATU_Dresden/Improve)
[Attributions](https://2017.igem.org/Team%3ATU_Dresden/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3ATU_Dresden/Parts)
[Basic Parts](https://2017.igem.org/Team%3ATU_Dresden/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3ATU_Dresden/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3ATU_Dresden/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3ATU_Dresden/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3ATU_Dresden/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3ATU_Dresden/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3ATU_Dresden/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3ATU_Dresden/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3ATU_Dresden/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3ATU_Dresden/Hardware)
[Measurement](https://2017.igem.org/Team%3ATU_Dresden/Measurement)
[Model](https://2017.igem.org/Team%3ATU_Dresden/Model)
[Plant](https://2017.igem.org/Team%3ATU_Dresden/Plant)
[Software](https://2017.igem.org/Team%3ATU_Dresden/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=TU_Dresden)

# TU\_Dresden

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

##### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

##### Inspiration

You can see what others teams have done to organize their notes:

* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)

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# Team:TU Dresden/Experiments

[![Logo](https://static.igem.org/mediawiki/2017/1/14/T--TU_Dresden--logo.png)](https://2017.igem.org/Team%3ATU_Dresden)

* [Project](https://2017.igem.org/Team%3ATU_Dresden/Description)
  + [Peptidosomes](https://2017.igem.org/Team%3ATU_Dresden/Project/Peptidosomes)
  + [β-Lactam Biosensor](https://2017.igem.org/Team%3ATU_Dresden/Project/Biosensor)
  + [Signal Peptide Toolbox](https://2017.igem.org/Team%3ATU_Dresden/Measurement)
  + [Evaluation Vector](https://2017.igem.org/Team%3ATU_Dresden/Composite_Part)
  + [Secretion](https://2017.igem.org/Team%3ATU_Dresden/Project/Secretion)
  + [Communication](https://2017.igem.org/Team%3ATU_Dresden/Project/Communication)
* [Results](https://2017.igem.org/Team%3ATU_Dresden/Results)
  + [Demonstration](https://2017.igem.org/Team%3ATU_Dresden/Demonstrate)
  + [Parts](https://2017.igem.org/Team%3ATU_Dresden/Parts)
* [Human Practices](https://2017.igem.org/Team%3ATU_Dresden/Human_Practices)
  + [Outreach](https://2017.igem.org/Team%3ATU_Dresden/HP/Silver)
  + [Integrated](https://2017.igem.org/Team%3ATU_Dresden/HP/Gold_Integrated)
  + [Collaborations](https://2017.igem.org/Team%3ATU_Dresden/Collaborations)
* [iGEM goes green](https://2017.igem.org/Team%3ATU_Dresden/iGEM-goes-green)
  + [Green Teams](https://2017.igem.org/Team%3ATU_Dresden/iGEM-goes-green/Green_Teams)
  + [Resources](https://2017.igem.org/Team%3ATU_Dresden/iGEM-goes-green/Resources)
  + [Our Achievements](https://2017.igem.org/Team%3ATU_Dresden/HP/Gold_Integrated)
  + [Our Experience](https://2017.igem.org/Team%3ATU_Dresden/iGEM-goes-green/Experience)
* [About Us](https://2017.igem.org/Team%3ATU_Dresden/About_Us)
  + [Team](https://2017.igem.org/Team%3ATU_Dresden/Team)
  + [Attributions](https://2017.igem.org/Team%3ATU_Dresden/Attributions)
  + [Financing & Sponsors](https://2017.igem.org/Team%3ATU_Dresden/Sponsors)
* [Lab Work](https://2017.igem.org/Team%3ATU_Dresden/Lab_Work)
  + [Experiments & Protocols](https://2017.igem.org/Team%3ATU_Dresden/Experiments)
  + [Safety](https://2017.igem.org/Team%3ATU_Dresden/Safety)
* [Judging](https://2017.igem.org/Team%3ATU_Dresden/Judging)

Don´t try this at home

# Peptidosome Methods

[Peptidosome Creation](https://static.igem.org/mediawiki/2017/8/87/Protocols_peptidosomes.pdf)
[Encapsulation of *B. subtilis*](https://static.igem.org/mediawiki/2017/f/f6/T--TU_Dresden--EncapsulationofBsubtilis.pdf)
[Plate Reader Wellscan Peptidosomes](https://static.igem.org/mediawiki/2017/4/41/Wellscan.pdf)

# Cloning and Primer Lists

## Cloning

[Cloning Procedure](https://static.igem.org/mediawiki/2017/d/dc/New_cloning.pdf)
[Transformation CaCl Method](https://static.igem.org/mediawiki/2017/3/39/Transformations.pdf)
[PCR with One Taq Polymerase](https://static.igem.org/mediawiki/2017/0/09/T--TU_Dresden--M_OneTaqPCR.pdf)
[PCR with Q5 Polymerase](https://static.igem.org/mediawiki/2017/1/17/T--TU_Dresden--M_Q5PCR.pdf)
[Long Flanking Homology PCR](https://static.igem.org/mediawiki/2017/b/bf/T--TU_Dresden--M_LFH.pdf)
[SOP Protocols for Cloning with the Evaluation Vector and the Signal Peptide Toolbox](https://static.igem.org/mediawiki/2017/d/db/SOP_protocols_for_cloning_with_the_Evaluation_Vector_and_the_Signal_Peptide_Toolbox.pdf)

## Primer

[Primer List General](https://static.igem.org/mediawiki/2017/0/0f/Primer_list.pdf)
[Primer List Signal Peptides](https://static.igem.org/mediawiki/2017/b/b8/Primer_list_signal_peptides.pdf)

# Working with *Bacillus subtilis*

[DNA Isolation (for PCR)](https://static.igem.org/mediawiki/2017/0/05/Dna_isolation_for_pcr.pdf)
[DNA Isolation (for Transformation)](https://static.igem.org/mediawiki/2017/4/4c/Dna_isolation_for_transformation.pdf)
[Transformation of *B. subtilis*](https://static.igem.org/mediawiki/2017/5/5f/Bacillus_transformation.pdf)

# Media and Solutions

[Media, Solutions & Antibiotics](https://static.igem.org/mediawiki/2017/6/62/Media_antibiotics.pdf)
[Jensen's Medium](https://static.igem.org/mediawiki/2017/2/29/Jensens_media.pdf)
[MCSE Medium](https://static.igem.org/mediawiki/2017/0/03/Mcse.pdf)

# Assays

[Disc Diffussion Assay](https://static.igem.org/mediawiki/2017/c/c8/Disc_diffusion.pdf)
[Plate Reader Assay](https://static.igem.org/mediawiki/2017/c/c4/Pr.pdf)
[Fluorescence Assay](https://static.igem.org/mediawiki/2017/3/3b/Secretion_assay.pdf)

# Protein Work

[His-Tag Purification with Agarose Beads](https://static.igem.org/mediawiki/2017/d/d9/Proteins.pdf)
[Laemmli SDS-PAGE](https://static.igem.org/mediawiki/2017/f/fa/Laemmli_SDS-PAGE.pdf)

Beam me up!

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