iGEM 2017 — Team Evry_Paris-Saclay

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# Team:Evry Paris-Saclay/Notebook

IGEM Evry Paris-Saclay

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[iGEM Evry Paris-Saclay](#page-top) ![](https://static.igem.org/mediawiki/2017/a/a1/T--Evry_Paris-Saclay--medal--or.png)

* [Home](https://2017.igem.org/Team%3AEvry_Paris-Saclay)
* Project
  + [Project Description](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Applied_Design)
  + [Chemistry](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Chemistry)
  + [Biosensor](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Measurement)
  + [Screening](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Demonstrate)
  + [Bioproduction](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Bioproduction)
  + [Modeling](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Model)
  + [Parts](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Part)
* [Notebook](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Notebook)
  + [Lab](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Lab)
  + [Labbook](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Notebook)
  + [Protocols](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Experiments)
  + [Medal Criterias](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Medals)
* Human Practice
  + [Human Practices: Front Page](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Human_Practices)
  + [Human Practices: Silver](https://2017.igem.org/Team%3AEvry_Paris-Saclay/HP/Silver)
  + [Integrated Human Practices: Gold](https://2017.igem.org/Team%3AEvry_Paris-Saclay/HP/Gold_Integrated)
  + [Education & Public Engagement](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Engagement)
  + [Collaborations](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Collaborations)
* [Interlab](https://2017.igem.org/Team%3AEvry_Paris-Saclay/InterLab)
* About Us
  + [Team](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Team)
  + [Attributions](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Attributions)
  + [Sponsors](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Sponsors)

#### **Lab Book**

Your browser isn't supporting embedded pdf files. You can download the file
[here](https://static.igem.org/mediawiki/2017/c/c0/T--Evry_Paris-Saclay--lab--pdf.pdf).

### [Lab](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Lab)

### [Protocols](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Experiments)

## Let's Get In Touch!

---

* [![](https://static.igem.org/mediawiki/2017/d/d9/T--Evry_Paris-Saclay--logoGenopole.png)](https://www.genopole.fr/)
* [![](https://static.igem.org/mediawiki/2017/9/9e/T--Evry_Paris-Saclay--logoiSSB.png)](http://www.issb.genopole.fr)
* [![](https://static.igem.org/mediawiki/2017/2/2d/T--Evry_Paris-Saclay--Logo_Paris-Saclay_Dep-Life-Sci.jpg)](https://www.universite-paris-saclay.fr/fr/recherche/departement/sciences-de-la-vie-sdv)
* [![](https://static.igem.org/mediawiki/2017/e/e2/T--Evry_Paris-Saclay--logoEvry.png)](http://www.univ-evry.fr/)
* [![](https://static.igem.org/mediawiki/2017/c/c9/T--Evry_Paris-Saclay--logos--sponsors--ambfrus.png)](https://fr.usembassy.gov/fr/)
* [![](https://static.igem.org/mediawiki/2017/d/dd/T--Evry_Paris-Saclay--logos--sponsors--creditmutuel.png)](https://www.creditmutuel.fr/home/index.html)
* [![](https://static.igem.org/mediawiki/2017/7/75/T--Evry_Paris-Saclay--logos--sponsors--GPS.jpg)](https://www.grandparissud.fr/)
* [![](https://static.igem.org/mediawiki/2017/8/8f/T--Evry_Paris-Saclay--logoGeneious.jpg)](https://www.geneious.com/)
* [![](https://static.igem.org/mediawiki/2017/e/e5/T--Evry_Paris-Saclay--logos--sponsors--sigma.png)](https://www.sigmaaldrich.com/)
* [![](https://static.igem.org/mediawiki/2017/7/71/IDT.png)](https://eu.idtdna.com/site)
* [![](https://static.igem.org/mediawiki/2017/7/7f/DalhousieNlogo.png)](https://www.neb.com/)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)


[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Evry Paris-Saclay/Experiments

IGEM Evry Paris-Saclay

Toggle navigation Menu
[iGEM Evry Paris-Saclay](#page-top) ![](https://static.igem.org/mediawiki/2017/a/a1/T--Evry_Paris-Saclay--medal--or.png)

* [Home](https://2017.igem.org/Team%3AEvry_Paris-Saclay)
* Project
  + [Project Description](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Applied_Design)
  + [Chemistry](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Chemistry)
  + [Biosensor](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Measurement)
  + [Screening](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Demonstrate)
  + [Bioproduction](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Bioproduction)
  + [Modeling](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Model)
  + [Parts](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Part)
* [Notebook](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Notebook)
  + [Lab](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Lab)
  + [Labbook](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Notebook)
  + [Protocols](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Experiments)
  + [Medal Criterias](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Medals)
* Human Practice
  + [Human Practices: Front Page](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Human_Practices)
  + [Human Practices: Silver](https://2017.igem.org/Team%3AEvry_Paris-Saclay/HP/Silver)
  + [Integrated Human Practices: Gold](https://2017.igem.org/Team%3AEvry_Paris-Saclay/HP/Gold_Integrated)
  + [Education & Public Engagement](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Engagement)
  + [Collaborations](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Collaborations)
* [Interlab](https://2017.igem.org/Team%3AEvry_Paris-Saclay/InterLab)
* About Us
  + [Team](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Team)
  + [Attributions](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Attributions)
  + [Sponsors](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Sponsors)

# Protocols

On this page, you can find the protocols we used during de iGEM project. Feel free to have a look.

---

#### **1. Digestion**

Your browser isn't supporting embedded pdf files. You can download the file
[here](https://static.igem.org/mediawiki/2017/5/52/T--Evry_Paris-Saclay--protocol--pdf--digestion.pdf).

#### **2. Ligation**

Your browser isn't supporting embedded pdf files. You can download the file
[here](https://static.igem.org/mediawiki/2017/7/71/T--Evry_Paris-Saclay--protocol--pdf--ligation.pdf).

#### **3. PCR**

Your browser isn't supporting embedded pdf files. You can download the file
[here](https://static.igem.org/mediawiki/2017/6/69/T--Evry_Paris-Saclay--protocol--pdf--pcr.pdf).

#### **4. Transformation**

Your browser isn't supporting embedded pdf files. You can download the file
[here](https://static.igem.org/mediawiki/2017/1/1b/T--Evry_Paris-Saclay--protocol--pdf--transfo.pdf).

#### **5. PCR Colony**

Your browser isn't supporting embedded pdf files. You can download the file
[here](https://static.igem.org/mediawiki/2017/0/05/T--Evry_Paris-Saclay--protocol--pdf--pcrcolony.pdf).

#### **6. Gel electrophoresis**

Your browser isn't supporting embedded pdf files. You can download the file
[here](https://static.igem.org/mediawiki/2017/f/f7/T--Evry_Paris-Saclay--protocol--pdf--gel.pdf).

#### **7. Pre Culture**

Your browser isn't supporting embedded pdf files. You can download the file
[here](https://static.igem.org/mediawiki/2017/8/8d/T--Evry_Paris-Saclay--protocol--pdf--culture.pdf).

#### **8. Glycerol**

Your browser isn't supporting embedded pdf files. You can download the file
[here](https://static.igem.org/mediawiki/2017/8/8e/T--Evry_Paris-Saclay--protocol--pdf--stock.pdf).

#### **9. PCR QC**

Your browser isn't supporting embedded pdf files. You can download the file
[here](https://static.igem.org/mediawiki/2017/9/9d/T--Evry_Paris-Saclay--protocol--pdf--pcrqc.pdf).

#### **10. Golden Gate**

Your browser isn't supporting embedded pdf files. You can download the file
[here](https://static.igem.org/mediawiki/2017/e/e8/T--Evry_Paris-Saclay--protocol--pdf--gate.pdf).

#### **11. Screening**

Your browser isn't supporting embedded pdf files. You can download the file
[here](https://static.igem.org/mediawiki/2017/f/fa/T--Evry_Paris-Saclay--protocol--pdf--screening.pdf).

#### **12. Characterization**

Your browser isn't supporting embedded pdf files. You can download the file
[here](https://static.igem.org/mediawiki/2017/5/5e/T--Evry_Paris-Saclay--protocol--pdf--chara.pdf).

### [Lab book](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Notebook)

### [Medal Criteria](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Medals)

## Let's Get In Touch!

---

* [![](https://static.igem.org/mediawiki/2017/d/d9/T--Evry_Paris-Saclay--logoGenopole.png)](https://www.genopole.fr/)
* [![](https://static.igem.org/mediawiki/2017/9/9e/T--Evry_Paris-Saclay--logoiSSB.png)](http://www.issb.genopole.fr)
* [![](https://static.igem.org/mediawiki/2017/2/2d/T--Evry_Paris-Saclay--Logo_Paris-Saclay_Dep-Life-Sci.jpg)](https://www.universite-paris-saclay.fr/fr/recherche/departement/sciences-de-la-vie-sdv)
* [![](https://static.igem.org/mediawiki/2017/e/e2/T--Evry_Paris-Saclay--logoEvry.png)](http://www.univ-evry.fr/)
* [![](https://static.igem.org/mediawiki/2017/c/c9/T--Evry_Paris-Saclay--logos--sponsors--ambfrus.png)](https://fr.usembassy.gov/fr/)
* [![](https://static.igem.org/mediawiki/2017/d/dd/T--Evry_Paris-Saclay--logos--sponsors--creditmutuel.png)](https://www.creditmutuel.fr/home/index.html)
* [![](https://static.igem.org/mediawiki/2017/7/75/T--Evry_Paris-Saclay--logos--sponsors--GPS.jpg)](https://www.grandparissud.fr/)
* [![](https://static.igem.org/mediawiki/2017/8/8f/T--Evry_Paris-Saclay--logoGeneious.jpg)](https://www.geneious.com/)
* [![](https://static.igem.org/mediawiki/2017/e/e5/T--Evry_Paris-Saclay--logos--sponsors--sigma.png)](https://www.sigmaaldrich.com/)
* [![](https://static.igem.org/mediawiki/2017/7/71/IDT.png)](https://eu.idtdna.com/site)
* [![](https://static.igem.org/mediawiki/2017/7/7f/DalhousieNlogo.png)](https://www.neb.com/)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Evry Paris-Saclay/Results

IGEM Evry Paris-Saclay

Toggle navigation Menu
[iGEM Evry Paris-Saclay](#page-top) ![](https://static.igem.org/mediawiki/2017/a/a1/T--Evry_Paris-Saclay--medal--or.png)

* [Home](https://2017.igem.org/Team%3AEvry_Paris-Saclay)
* Project
  + [Project Description](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Applied_Design)
  + [Chemistry](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Chemistry)
  + [Biosensor](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Measurement)
  + [Screening](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Demonstrate)
  + [Bioproduction](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Bioproduction)
  + [Modeling](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Model)
  + [Parts](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Part)
* [Notebook](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Notebook)
  + [Lab](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Lab)
  + [Labbook](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Notebook)
  + [Protocols](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Experiments)
  + [Medal Criterias](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Medals)
* Human Practice
  + [Human Practices: Front Page](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Human_Practices)
  + [Human Practices: Silver](https://2017.igem.org/Team%3AEvry_Paris-Saclay/HP/Silver)
  + [Integrated Human Practices: Gold](https://2017.igem.org/Team%3AEvry_Paris-Saclay/HP/Gold_Integrated)
  + [Education & Public Engagement](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Engagement)
  + [Collaborations](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Collaborations)
* [Interlab](https://2017.igem.org/Team%3AEvry_Paris-Saclay/InterLab)
* About Us
  + [Team](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Team)
  + [Attributions](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Attributions)
  + [Sponsors](https://2017.igem.org/Team%3AEvry_Paris-Saclay/Sponsors)

# Results

Here you can describe the results of your project and your future plans.

##### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

##### You should also describe what your results mean:

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

##### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

##### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)

## Let's Get In Touch!

---

* [![](https://static.igem.org/mediawiki/2017/d/d9/T--Evry_Paris-Saclay--logoGenopole.png)](https://www.genopole.fr/)
* [![](https://static.igem.org/mediawiki/2017/9/9e/T--Evry_Paris-Saclay--logoiSSB.png)](http://www.issb.genopole.fr)
* [![](https://static.igem.org/mediawiki/2017/2/2d/T--Evry_Paris-Saclay--Logo_Paris-Saclay_Dep-Life-Sci.jpg)](https://www.universite-paris-saclay.fr/fr/recherche/departement/sciences-de-la-vie-sdv)
* [![](https://static.igem.org/mediawiki/2017/e/e2/T--Evry_Paris-Saclay--logoEvry.png)](http://www.univ-evry.fr/)
* [![](https://static.igem.org/mediawiki/2017/c/c9/T--Evry_Paris-Saclay--logos--sponsors--ambfrus.png)](https://fr.usembassy.gov/fr/)
* [![](https://static.igem.org/mediawiki/2017/d/dd/T--Evry_Paris-Saclay--logos--sponsors--creditmutuel.png)](https://www.creditmutuel.fr/home/index.html)
* [![](https://static.igem.org/mediawiki/2017/7/75/T--Evry_Paris-Saclay--logos--sponsors--GPS.jpg)](https://www.grandparissud.fr/)
* [![](https://static.igem.org/mediawiki/2017/8/8f/T--Evry_Paris-Saclay--logoGeneious.jpg)](https://www.geneious.com/)
* [![](https://static.igem.org/mediawiki/2017/e/e5/T--Evry_Paris-Saclay--logos--sponsors--sigma.png)](https://www.sigmaaldrich.com/)
* [![](https://static.igem.org/mediawiki/2017/7/71/IDT.png)](https://eu.idtdna.com/site)
* [![](https://static.igem.org/mediawiki/2017/7/7f/DalhousieNlogo.png)](https://www.neb.com/)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)