iGEM 2017 — Team Tec-Chihuahua

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Tec-Chihuahua/Notebook

[![](http://placehold.it/350x150)](https://2017.igem.org/Team%3ATec-Chihuahua)

MENU

[HOME](https://2017.igem.org/Team%3ATec-Chihuahua)

TEAM

[Team](https://2017.igem.org/Team%3ATec-Chihuahua/Team)
[Collaborations](https://2017.igem.org/Team%3ATec-Chihuahua/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3ATec-Chihuahua/Description)
[Design](https://2017.igem.org/Team%3ATec-Chihuahua/Design)
[Experiments](https://2017.igem.org/Team%3ATec-Chihuahua/Experiments)
[Notebook](https://2017.igem.org/Team%3ATec-Chihuahua/Notebook)
[InterLab](https://2017.igem.org/Team%3ATec-Chihuahua/InterLab)
[Contribution](https://2017.igem.org/Team%3ATec-Chihuahua/Contribution)
[Model](https://2017.igem.org/Team%3ATec-Chihuahua/Model)
[Results](https://2017.igem.org/Team%3ATec-Chihuahua/Results)
[Demonstrate](https://2017.igem.org/Team%3ATec-Chihuahua/Demonstrate)
[Improve](https://2017.igem.org/Team%3ATec-Chihuahua/Improve)
[Attributions](https://2017.igem.org/Team%3ATec-Chihuahua/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3ATec-Chihuahua/Parts)
[Basic Parts](https://2017.igem.org/Team%3ATec-Chihuahua/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3ATec-Chihuahua/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3ATec-Chihuahua/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3ATec-Chihuahua/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3ATec-Chihuahua/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3ATec-Chihuahua/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3ATec-Chihuahua/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3ATec-Chihuahua/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3ATec-Chihuahua/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3ATec-Chihuahua/Hardware)
[Measurement](https://2017.igem.org/Team%3ATec-Chihuahua/Measurement)
[Model](https://2017.igem.org/Team%3ATec-Chihuahua/Model)
[Plant](https://2017.igem.org/Team%3ATec-Chihuahua/Plant)
[Software](https://2017.igem.org/Team%3ATec-Chihuahua/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=Tec-Chihuahua)

# Tec-Chihuahua

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

##### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

##### Inspiration

You can see what others teams have done to organize their notes:

* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)


[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Tec-Chihuahua/Experiments

[![](http://placehold.it/350x150)](https://2017.igem.org/Team%3ATec-Chihuahua)

MENU

[HOME](https://2017.igem.org/Team%3ATec-Chihuahua)

TEAM

[Team](https://2017.igem.org/Team%3ATec-Chihuahua/Team)
[Collaborations](https://2017.igem.org/Team%3ATec-Chihuahua/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3ATec-Chihuahua/Description)
[Design](https://2017.igem.org/Team%3ATec-Chihuahua/Design)
[Experiments](https://2017.igem.org/Team%3ATec-Chihuahua/Experiments)
[Notebook](https://2017.igem.org/Team%3ATec-Chihuahua/Notebook)
[InterLab](https://2017.igem.org/Team%3ATec-Chihuahua/InterLab)
[Contribution](https://2017.igem.org/Team%3ATec-Chihuahua/Contribution)
[Model](https://2017.igem.org/Team%3ATec-Chihuahua/Model)
[Results](https://2017.igem.org/Team%3ATec-Chihuahua/Results)
[Demonstrate](https://2017.igem.org/Team%3ATec-Chihuahua/Demonstrate)
[Improve](https://2017.igem.org/Team%3ATec-Chihuahua/Improve)
[Attributions](https://2017.igem.org/Team%3ATec-Chihuahua/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3ATec-Chihuahua/Parts)
[Basic Parts](https://2017.igem.org/Team%3ATec-Chihuahua/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3ATec-Chihuahua/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3ATec-Chihuahua/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3ATec-Chihuahua/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3ATec-Chihuahua/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3ATec-Chihuahua/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3ATec-Chihuahua/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3ATec-Chihuahua/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3ATec-Chihuahua/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3ATec-Chihuahua/Hardware)
[Measurement](https://2017.igem.org/Team%3ATec-Chihuahua/Measurement)
[Model](https://2017.igem.org/Team%3ATec-Chihuahua/Model)
[Plant](https://2017.igem.org/Team%3ATec-Chihuahua/Plant)
[Software](https://2017.igem.org/Team%3ATec-Chihuahua/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=Tec-Chihuahua)

# Tec-Chihuahua

Trial

[ ]
![](https://d30y9cdsu7xlg0.cloudfront.net/png/356889-200.png)

* [HOME](index.html)
* [PROJECT](project.html)
  + ATTRIBUTIONS
  + CONTRIBUTION
  + DEMONSTRATE
  + DESCRIPTION
  + DESIGN
  + EXPERIMENTS
  + IMPROVE
  + INTERNLAB
  + MODEL
  + NOTEBOOK
  + RESULTS
* [TEAM](team.html)
  + TEAM
  + COLLABORATIONS
* [AWARDS](awards.html)
  + APPLIED DESIGN
  + ENTREPRENEURSHIP
  + HARDWARE
  + MEASUREMENT
  + MODEL
  + PLANT
  + SOFTWARE
* [HUMAN PRACTICES](human_practices.html)
  + INTEGRATED PRACTICES
  + PUBLIC ENGAGEMENT
  + SILVER
* [JUDGING FORM](judgingform.html)
* [PARTS](parts.html)
  + PARTS
  + BASIC PARTS
  + COMPOSITE PARTS
  + PART COLLECTION
* [SAFETY](safety.html)

# Project

###

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Tec-Chihuahua/Results

Erwinions

* [HOME](//2017.igem.org/Team%3ATec-Chihuahua)
* [TEAM](//2017.igem.org/Team%3ATec-Chihuahua/team)
  + [MEMBERS](//2017.igem.org/Team%3ATec-Chihuahua/team)
  + [COLLABORATIONS](//2017.igem.org/Team%3ATec-Chihuahua/Collaborations)
  + [ATTRIBUTIONS](//2017.igem.org/Team%3ATec-Chihuahua/Attributions)
* PROJECT
  + [DESCRIPTION](//2017.igem.org/Team%3ATec-Chihuahua/description)
  + [DESIGN](//2017.igem.org/Team%3ATec-Chihuahua/Applied_Design)
  + [PARTS](//2017.igem.org/Team%3ATec-Chihuahua/Parts)
  + [RESULTS](//2017.igem.org/Team%3ATec-Chihuahua/Results)
* NOTEBOOK
  + [BRAINSTORM](//2017.igem.org/Team%3ATec-Chihuahua/brainstorm)
  + [LAB BOOK](//2017.igem.org/Team%3ATec-Chihuahua/lab_book)
  + [PROTOCOLS](//2017.igem.org/Team%3ATec-Chihuahua/protocols)
* HUMAN PRACTICES
  + [HUMAN PRACTICES](//2017.igem.org/Team%3ATec-Chihuahua/HP)
  + [INTEGRATED PRACTICES](https://2017.igem.org/Team%3ATec-Chihuahua/HP/Gold_Integrated)
  + [PUBLIC ENGAGEMENT](//2017.igem.org/Team%3ATec-Chihuahua/Engagement)
* MODEL
  + [OVERVIEW](//2017.igem.org/Team%3ATec-Chihuahua/overview)
  + [WILD CELL](//2017.igem.org/Team%3ATec-Chihuahua/wildcell)
  + [MODIFIED CELL](//2017.igem.org/Team%3ATec-Chihuahua/ModifiedCell)
  + [RESULTS](//2017.igem.org/Team%3ATec-Chihuahua/Model)
* ENTREPRENEURSHIP
  + [OVERVIEW](//2017.igem.org/Team%3ATec-Chihuahua/EntOverview)
  + [ERWINIONS](//2017.igem.org/Team%3ATec-Chihuahua/Entrepreneurship)
  + [LEAN METHOD](//2017.igem.org/Team%3ATec-Chihuahua/process_lean_method)* JUDGING
    + [AWARDS](//2017.igem.org/Team%3ATec-Chihuahua/awards)
    + [SAFETY](//2017.igem.org/Team%3ATec-Chihuahua/Safety)

# Results

### General Introduction

As described on the Design section, we developed a laboratory methodology to accomplish our proof of concept. Fortunately, thanks to different factors, this methodology helped us accomplish all of the bronze, silver, and gold requirements. For example, there were some parts that were already indexed in the iGEM Parts Registry, but lacked characterization. Some others were reported as putative genes on NCBI, which gave us enough reasons to create new parts, meeting the silver criteria. Finally, thanks to our  [Integrated Human Practices](https://2017.igem.org/Team%3ATec-Chihuahua/HP/Gold_Integrated) and [Entrepreneurship](https://2017.igem.org/Team%3ATec-Chihuahua/EntOverview), we were able to establish a demonstration protocol that meets the necessities of a client or posible investor, like "La Norteñita" (leading company in apple production and commercialization).

The characterizations that were carried out were both at a molecular and cellular level. For example, we did a protein expression kinetic (bronze and silver), an adherence capacity test (improve), and an apple tree leaves inoculation protocol (demonstrate). You can read more about the results by clicking on the buttons below.

[**BRONZE**](//2017.igem.org/Team%3ATec-Chihuahua/Contribution)

[**SILVER**](//2017.igem.org/Team%3ATec-Chihuahua/Silver)

[**IMPROVE**](//2017.igem.org/Team%3ATec-Chihuahua/Improve)

[**DEMONSTRATE**](//2017.igem.org/Team%3ATec-Chihuahua/Demonstrate)

### Follow us on social media:

![](https://static.igem.org/mediawiki/2017/e/ee/T--Tec-Chihuahua--FaceB.png)

Facebook: [facebook.com/igemcuu/](https://www.facebook.com/igemcuu/)

![](https://static.igem.org/mediawiki/2017/4/49/T--Tec-Chihuahua--Twitter.png)

Twitter: [@iGEM\_TecChih](https://twitter.com/iGEM_TecChih)

![](https://static.igem.org/mediawiki/2017/9/90/T--Tec-Chihuahua--Gmail.png)

Email: [igemtecchihuahua@gmail.com](igemtecchihuahua%40gmail.com)

### We thank our sponsors:

[![](https://static.igem.org/mediawiki/2017/a/af/T--Tec-Chihuahua--LogoTermo.png)](https://www.thermofisher.com/mx/es/home.html)

[![](https://static.igem.org/mediawiki/2017/e/e2/T--Tec-Chihuahua--Vanilla.jpg)](https://studiovainilla.com/)

[![](https://static.igem.org/mediawiki/2017/7/72/T--Tec-Chihuahua--LogoNewE.png)](https://www.neb.com/)

[![](https://static.igem.org/mediawiki/2017/0/07/T--Tec-Chihuahua--Logosnap.png)](http://www.snapgene.com/)

[![](https://static.igem.org/mediawiki/2017/a/a8/T--Tec-Chihuahua--LogoAgilent.png)](https://www.agilent.com/home)

[![](https://static.igem.org/mediawiki/2017/4/42/T--Tec-Chihuahua--logoidt.jpg)](https://www.idtdna.com/site)

[![](https://static.igem.org/mediawiki/2017/c/c2/T--Tec-Chihuahua--logomathworks.jpeg)](https://es.mathworks.com/)

[![](https://static.igem.org/mediawiki/2017/1/16/T--Tec-Chihuahua--Teclogo2.png)](https://www.chi.itesm.mx/)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)