iGEM 2015 — Team Bielefeld-CeBiTec

# Team:Bielefeld-CeBiTec/Notebook

iGEM Bielefeld 2015

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[![](https://static.igem.org/mediawiki/2015/0/07/Bielefeld-CeBiTec_Logo_small.png)](https://2015.igem.org/Team%3ABielefeld-CeBiTec)

[![](https://static.igem.org/mediawiki/2015/0/07/Bielefeld-CeBiTec_Logo_small.png)](https://2015.igem.org/Team%3ABielefeld-CeBiTec)* Project
  + [Overview](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Overview)
  + Biosensor Design
    - [Motivation](https://2015.igem.org/Team%3ABielefeld-CeBiTec/BiosensorDesignMotivation)
    - [Cell Free Protein Synthesis](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Project/CFPS)
    - [Plasmid Repressor Interaction Assay](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Project/PRIA)
    - [Biosafety](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Project/Biosafety)
  + Biosensor Targets
    - [Motivation](https://2015.igem.org/Team%3ABielefeld-CeBiTec/BiosensorFunctionMotivation)
    - [Heavy Metals](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Project/HeavyMetals)
    - [Date Rape Drugs](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Project/DateRapeDrugs)
    - [Foundations](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Description)
  + [Output signal](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Project/Detection)
* Results
  + [Overview](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Results)
  + Design Evolution
    - [Cell Free Protein Synthesis](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Results/CFPS)
    - [Plasmid Repressor Interaction Assay](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Results/PRIA)
  + Biosensor Characterization
    - [Heavy Metals](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Results/HeavyMetals)
    - [Date Rape Drugs](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Results/DateRapeDrugs)
  + [Output Signal Processing](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Design)
  + [ALL-IN-ONE Performance](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Results/it-really-works)
  + [Parts](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Parts)
  + [Interlab Study](https://2015.igem.org/Team%3ABielefeld-CeBiTec/InterlabStudy)
  + [Achievements](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Achievements)
* Modeling
  + [Overview](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Modeling)
  + [CFPS Model](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Modeling/CFPS)
  + [Biosensor Model](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Modeling/Biosensor)
  + [Model Applications](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Modeling/Application)
* Team
  + [Team Members](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Team)
  + [Attributions](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Attributions)
  + [Bielefeld & University](https://2015.igem.org/Team%3ABielefeld-CeBiTec/BielefeldUniversity)
  + [Contact](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Contact)
* Human Practices
  + [Overview](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Practices)
  + [Dual Use](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Practices/DualUse)
  + [Future Scenarios](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Practices/Scenarios)
  + [Street Science](https://2015.igem.org/Team%3ABielefeld-CeBiTec/StreetScience)
  + [Students Academy](https://2015.igem.org/Team%3ABielefeld-CeBiTec/StudentsAcademy)
  + [Press Review](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Practices/PressReview)
* Notebook
  + [Lab Notebook](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Notebook)
  + [Protocols](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Protocols)
  + [Media & Buffers](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Media)
  + [Primers](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Primers)
  + [Organisms](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Organisms)
* Partners
  + [Sponsors](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Sponsors)
  + [Partners](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Partners)
  + [Collaborations](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Collaborations)
[![](https://static.igem.org/mediawiki/2015/5/51/Bielefeld-CeBiTec_iGEM_logo.png)](https://2015.igem.org/Main_Page)

# Lab Notebook

Here you can find chronological notes of our lab work, sorted by subtopics.

[![Heavy Metals](https://static.igem.org/mediawiki/2015/e/e0/Bielefeld-Cebitec-Notebook-Minipic-HeavyMetals_200x200.JPG)

## Heavy metals

Here you find some detailed information about the lab work concerning the construction of our heavy metal biosensors in *E. coli*. You can see the difficulties we ran into and follow our progress in creating our sensor systems.](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Notebook/HeavyMetals)

[![Generic placeholder image](https://static.igem.org/mediawiki/2015/1/10/Bielefeld-Cebitec-Notebook-Minipic-KO-tropfen_200x200.JPG)

## Date rape drugs

The detailed process of the construction and characterization of the sensors detecting GBL, GHB and GABA in *E. coli*, is shown. You find our various methods to obtain the necessary elements from gene synthesis, *A. tumifaciens* and *B. subtilis*.](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Notebook/DRdrugs)

[![CFPS](https://static.igem.org/mediawiki/2015/9/9c/Bielefeld-Cebitec-Notebook-Minipic-CFPS_200x200.JPG)

## CFPS

In cell free protein synthesis (CFPS), the extracts of lysed cells, containing all the necessary components for transcription and translation, are used to produce proteins *in vitro*. Find out how we developed and optimized our own cell extract.](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Notebook/CFPS)

[![Generic placeholder image](https://static.igem.org/mediawiki/2015/0/07/Bielefeld-Cebitec-Notebook-Minipic-PRIA_200x200.JPG)

## PRIA

See how we developed this alternative to standard biosensors, which is based on *in vitro* interaction of immobilized repressor proteins with plasmids containing the operator site corresponding to the repressor.](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Notebook/PlasmidDisplay)

[![Generic placeholder image](https://static.igem.org/mediawiki/2015/0/0b/Bielefeld-Cebitec-Notebook-Minipic-Verschiedened_200x200.JPG)

## Miscellaneous

Here you can find chronological notes of our lab work besides the main topics, e.g. including the Measurement Interlab Study 2015.](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Notebook/Miscellaneous)

[![](https://static.igem.org/mediawiki/2015/b/bc/Bielefeld-CeBiTec_notebook_logo.png)

Protocols](https://2015.igem.org/Team%3ABielefeld-CeBiTec/Protocols)