iGEM 2024 — Team rbhs-san-diego

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{% block title %}Notebook{% endblock %}
{% block lead %}Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.{% endblock %}
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## Lab Notebook

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## Meeting Notes

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{% block title %}Engineering Success{% endblock %}
{% block lead %}Designing, building, testing, and learning{% endblock %}
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{% block title %}Experiments{% endblock %}
{% block lead %}The research, experiments, and protocols we used.{% endblock %}
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## Lab Protocol

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Additional information on lab results can be seen under [Results](%7B%7B%20url_for%28%27pages%27%2C%20page%3D%27results%27%29%20%7D%7D) and [Engineering Success](%7B%7B%20url_for%28%27pages%27%2C%20page%3D%27results%27%29%20%7D%7D).

All lab procedures were carried out at the Love Lab at San Diego State University under the supervision of Dr. John Love and Ph.D. student Sierra Murrell. We used the standard operating procedures of the Love Lab, and we would like to thank them once again for allowing us to work on our project using their equipment, reagents, and guidance.

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{% block title %}Results{% endblock %}
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## Overview

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We completed PCR, DNA clean and concentrate, digestion, DNA recovery, ligation, and transformation for the 6-4 photolyase gene fragment from Arabidopsis thaliana in TOP10 E. coli cells. We had to repeat the entire process three times to yield a successful bacterial transformation. See Engineering Success and Lab Notebook for further details.

Additional information on lab results can be seen under [Results](%7B%7B%20url_for%28%27pages%27%2C%20page%3D%27results%27%29%20%7D%7D) and [Engineering Success](%7B%7B%20url_for%28%27pages%27%2C%20page%3D%27results%27%29%20%7D%7D).

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