iGEM 2019 — Team MIT

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:MIT/Notebook

[HOME](https://2019.igem.org/Team%3AMIT)

[TEAM](https://2019.igem.org/Team%3AMIT/Team)

PROJECT

[Description](https://2019.igem.org/Team%3AMIT/Description)

[Design](https://2019.igem.org/Team%3AMIT/Design)

[Experiments](https://2019.igem.org/Team%3AMIT/Experiments)

[Notebook](https://2019.igem.org/Team%3AMIT/Notebook)

[Model](https://2019.igem.org/Team%3AMIT/Model)

[Results](https://2019.igem.org/Team%3AMIT/Results)

[Demonstrate](https://2019.igem.org/Team%3AMIT/Demonstrate)

[Improve](https://2019.igem.org/Team%3AMIT/Improve)

[Attributions](https://2019.igem.org/Team%3AMIT/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3AMIT/Parts)

[Basic Parts](https://2019.igem.org/Team%3AMIT/Basic_Part)

[Composite Parts](https://2019.igem.org/Team%3AMIT/Composite_Part)

[Part Collection](https://2019.igem.org/Team%3AMIT/Part_Collection)

[Characterization](https://2019.igem.org/Team%3AMIT/Contribution)

[SAFETY](https://2019.igem.org/Team%3AMIT/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3AMIT/Human_Practices)

[Education & Engagement](https://2019.igem.org/Team%3AMIT/Public_Engagement)

[NEGEM](https://2019.igem.org/Team%3AMIT/NEGEM)

# Notebook

Our project consists of four major components which also formed the basis for our subteams. We document our thought process and how we developed our project goals, timeline, and experiments in the [Design Notebook](https://2019.igem.org/Team%3AMIT/Design) and our progress each day for each respective subteam in the Cloning, Culture, and Assay Notebooks.

Cloning Notebook

1.
[![](http://www.iiakm.org/ojakm/images/PDF.jpg) Cloning Notebook (PDF file)](https://static.igem.org/mediawiki/2019/2/20/T--MIT----Notebook%28Cloning%29.pdf)

Culturing Notebook

2.
[![](http://www.iiakm.org/ojakm/images/PDF.jpg) Culturing Notebook (PDF File)](https://static.igem.org/mediawiki/2019/f/f0/T--MIT----Notebook%28Culturing%29.pdf)

Assay Notebook

3.
[![](http://www.iiakm.org/ojakm/images/PDF.jpg) Assay Notebook (PDF File)](https://static.igem.org/mediawiki/2019/3/31/T--MIT----Notebook%28Assay%29.pdf)


[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:MIT/Experiments

[HOME](https://2019.igem.org/Team%3AMIT)

[TEAM](https://2019.igem.org/Team%3AMIT/Team)

PROJECT

[Description](https://2019.igem.org/Team%3AMIT/Description)

[Design](https://2019.igem.org/Team%3AMIT/Design)

[Experiments](https://2019.igem.org/Team%3AMIT/Experiments)

[Notebook](https://2019.igem.org/Team%3AMIT/Notebook)

[Model](https://2019.igem.org/Team%3AMIT/Model)

[Results](https://2019.igem.org/Team%3AMIT/Results)

[Demonstrate](https://2019.igem.org/Team%3AMIT/Demonstrate)

[Improve](https://2019.igem.org/Team%3AMIT/Improve)

[Attributions](https://2019.igem.org/Team%3AMIT/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3AMIT/Parts)

[Basic Parts](https://2019.igem.org/Team%3AMIT/Basic_Part)

[Composite Parts](https://2019.igem.org/Team%3AMIT/Composite_Part)

[Part Collection](https://2019.igem.org/Team%3AMIT/Part_Collection)

[Characterization](https://2019.igem.org/Team%3AMIT/Contribution)

[SAFETY](https://2019.igem.org/Team%3AMIT/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3AMIT/Human_Practices)

[Education & Engagement](https://2019.igem.org/Team%3AMIT/Public_Engagement)

[NEGEM](https://2019.igem.org/Team%3AMIT/NEGEM)

# Experiments

Here are the protocols we followed for our experiments.

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:MIT/Results

[HOME](https://2019.igem.org/Team%3AMIT)

[TEAM](https://2019.igem.org/Team%3AMIT/Team)

PROJECT

[Description](https://2019.igem.org/Team%3AMIT/Description)

[Design](https://2019.igem.org/Team%3AMIT/Design)

[Experiments](https://2019.igem.org/Team%3AMIT/Experiments)

[Notebook](https://2019.igem.org/Team%3AMIT/Notebook)

[Model](https://2019.igem.org/Team%3AMIT/Model)

[Results](https://2019.igem.org/Team%3AMIT/Results)

[Demonstrate](https://2019.igem.org/Team%3AMIT/Demonstrate)

[Improve](https://2019.igem.org/Team%3AMIT/Improve)

[Attributions](https://2019.igem.org/Team%3AMIT/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3AMIT/Parts)

[Basic Parts](https://2019.igem.org/Team%3AMIT/Basic_Part)

[Composite Parts](https://2019.igem.org/Team%3AMIT/Composite_Part)

[Part Collection](https://2019.igem.org/Team%3AMIT/Part_Collection)

[Characterization](https://2019.igem.org/Team%3AMIT/Contribution)

[SAFETY](https://2019.igem.org/Team%3AMIT/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3AMIT/Human_Practices)

[Education & Engagement](https://2019.igem.org/Team%3AMIT/Public_Engagement)

[NEGEM](https://2019.igem.org/Team%3AMIT/NEGEM)

# Results

Below are our videos of our cells and a PDF with our results. Click the little icons to watch the videos

* [![](https://static.igem.org/mediawiki/2019/3/32/T--MIT--playbutton.png) Follower Leader (gif)](https://static.igem.org/mediawiki/2019/a/ad/T--MIT----Follower%2BLeader-gif.gif)
* [![](https://static.igem.org/mediawiki/2019/3/32/T--MIT--playbutton.png) Undifferentiated HL-60 Test (gif)](https://static.igem.org/mediawiki/2019/d/d5/T--MIT----Finished-undiff-HL60-test.gif)
* [![](https://static.igem.org/mediawiki/2019/3/32/T--MIT--playbutton.png) Differentiated HL-60 Test (gif)](https://static.igem.org/mediawiki/2019/8/86/T--MIT----Finished-diff-HL60_test.gif)