iGEM 2017 — Team SMS_Shenzhen

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# Team:SMS Shenzhen/Notebook

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MENU

[HOME](https://2017.igem.org/Team%3ASMS_Shenzhen)

TEAM

[Team](https://2017.igem.org/Team%3ASMS_Shenzhen/Team)
[Collaborations](https://2017.igem.org/Team%3ASMS_Shenzhen/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3ASMS_Shenzhen/Description)
[Design](https://2017.igem.org/Team%3ASMS_Shenzhen/Design)
[Experiments](https://2017.igem.org/Team%3ASMS_Shenzhen/Experiments)
[Notebook](https://2017.igem.org/Team%3ASMS_Shenzhen/Notebook)
[InterLab](https://2017.igem.org/Team%3ASMS_Shenzhen/InterLab)
[Contribution](https://2017.igem.org/Team%3ASMS_Shenzhen/Contribution)
[Model](https://2017.igem.org/Team%3ASMS_Shenzhen/Model)
[Results](https://2017.igem.org/Team%3ASMS_Shenzhen/Results)
[Demonstrate](https://2017.igem.org/Team%3ASMS_Shenzhen/Demonstrate)
[Improve](https://2017.igem.org/Team%3ASMS_Shenzhen/Improve)
[Attributions](https://2017.igem.org/Team%3ASMS_Shenzhen/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3ASMS_Shenzhen/Parts)
[Basic Parts](https://2017.igem.org/Team%3ASMS_Shenzhen/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3ASMS_Shenzhen/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3ASMS_Shenzhen/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3ASMS_Shenzhen/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3ASMS_Shenzhen/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3ASMS_Shenzhen/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3ASMS_Shenzhen/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3ASMS_Shenzhen/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3ASMS_Shenzhen/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3ASMS_Shenzhen/Hardware)
[Measurement](https://2017.igem.org/Team%3ASMS_Shenzhen/Measurement)
[Model](https://2017.igem.org/Team%3ASMS_Shenzhen/Model)
[Plant](https://2017.igem.org/Team%3ASMS_Shenzhen/Plant)
[Software](https://2017.igem.org/Team%3ASMS_Shenzhen/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=SMS_Shenzhen)

# SMS\_Shenzhen

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

##### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

##### Inspiration

You can see what others teams have done to organize their notes:

* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)


[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:SMS Shenzhen/Experiments

Title

* [Home](https://2017.igem.org/Team%3ASMS_Shenzhen)

  [Project](https://2017.igem.org/Team%3ASMS_Shenzhen/Description)

  [Description](https://2017.igem.org/Team%3ASMS_Shenzhen/Description)
  [Applied Design](https://2017.igem.org/Team%3ASMS_Shenzhen/Applied_Design)
  [Model](https://2017.igem.org/Team%3ASMS_Shenzhen/Model)
  [Demonstrate](https://2017.igem.org/Team%3ASMS_Shenzhen/Demonstrate)

  [Experiments](https://2017.igem.org/Team%3ASMS_Shenzhen/Experiments)

  [Summary](https://2017.igem.org/Team%3ASMS_Shenzhen/Experiments)
  [Results](https://2017.igem.org/Team%3ASMS_Shenzhen/Results)

  [Parts](https://2017.igem.org/Team%3ASMS_Shenzhen/Parts)

  [Summary](https://2017.igem.org/Team%3ASMS_Shenzhen/Parts)
  [Basic Parts](https://2017.igem.org/Team%3ASMS_Shenzhen/Basic_Part)
  [Composite Parts](https://2017.igem.org/Team%3ASMS_Shenzhen/Composite_Part)
  [Contribution](https://2017.igem.org/Team%3ASMS_Shenzhen/Contribution)

  [Collaborations](https://2017.igem.org/Team%3ASMS_Shenzhen/Collaborations)

  [Human Practice](https://2017.igem.org/Team%3ASMS_Shenzhen/Human_Practices)

  [Summary](https://2017.igem.org/Team%3ASMS_Shenzhen/Human_Practices)
  [Silver](https://2017.igem.org/Team%3ASMS_Shenzhen/HP/Silver)
  [Gold](https://2017.igem.org/Team%3ASMS_Shenzhen/HP/Gold_Integrated)
  [Engagement](https://2017.igem.org/Team%3ASMS_Shenzhen/Engagement)

  [Safety](https://2017.igem.org/Team%3ASMS_Shenzhen/Safety)

  [Team](https://2017.igem.org/Team%3ASMS_Shenzhen/Team)

  [Team Members](https://2017.igem.org/Team%3ASMS_Shenzhen/Team)
  [Attributions](https://2017.igem.org/Team%3ASMS_Shenzhen/Attributions)

Title

![](https://static.igem.org/mediawiki/2017/b/ba/ExperimentPicNew-SMS_Shenzhen.jpg)
[![](  https://static.igem.org/mediawiki/2017/9/95/Expt1_shenzhen.jpg )](https://2017.igem.org/Team%3ASMS_Shenzhen/Experiments/EnzymeActivity)
[![]( https://static.igem.org/mediawiki/2017/9/90/Expt2_shenzhen.jpg )](https://2017.igem.org/Team%3ASMS_Shenzhen/Contribution)
[![]( https://static.igem.org/mediawiki/2017/6/60/Jiucuo-SMS.jpg )](https://2017.igem.org/Team%3ASMS_Shenzhen/Demonstrate)
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[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:SMS Shenzhen/Results

Title

* [Home](https://2017.igem.org/Team%3ASMS_Shenzhen)

  [Project](https://2017.igem.org/Team%3ASMS_Shenzhen/Description)

  [Description](https://2017.igem.org/Team%3ASMS_Shenzhen/Description)
  [Applied Design](https://2017.igem.org/Team%3ASMS_Shenzhen/Applied_Design)
  [Model](https://2017.igem.org/Team%3ASMS_Shenzhen/Model)
  [Demonstrate](https://2017.igem.org/Team%3ASMS_Shenzhen/Demonstrate)

  [Experiments](https://2017.igem.org/Team%3ASMS_Shenzhen/Experiments)

  [Summary](https://2017.igem.org/Team%3ASMS_Shenzhen/Experiments)
  [Results](https://2017.igem.org/Team%3ASMS_Shenzhen/Results)

  [Parts](https://2017.igem.org/Team%3ASMS_Shenzhen/Parts)

  [Summary](https://2017.igem.org/Team%3ASMS_Shenzhen/Parts)
  [Basic Parts](https://2017.igem.org/Team%3ASMS_Shenzhen/Basic_Part)
  [Composite Parts](https://2017.igem.org/Team%3ASMS_Shenzhen/Composite_Part)
  [Contribution](https://2017.igem.org/Team%3ASMS_Shenzhen/Contribution)

  [Collaborations](https://2017.igem.org/Team%3ASMS_Shenzhen/Collaborations)

  [Human Practice](https://2017.igem.org/Team%3ASMS_Shenzhen/Human_Practices)

  [Summary](https://2017.igem.org/Team%3ASMS_Shenzhen/Human_Practices)
  [Silver](https://2017.igem.org/Team%3ASMS_Shenzhen/HP/Silver)
  [Gold](https://2017.igem.org/Team%3ASMS_Shenzhen/HP/Gold_Integrated)
  [Engagement](https://2017.igem.org/Team%3ASMS_Shenzhen/Engagement)

  [Safety](https://2017.igem.org/Team%3ASMS_Shenzhen/Safety)

  [Team](https://2017.igem.org/Team%3ASMS_Shenzhen/Team)

  [Team Members](https://2017.igem.org/Team%3ASMS_Shenzhen/Team)
  [Attributions](https://2017.igem.org/Team%3ASMS_Shenzhen/Attributions)

Title

![](https://static.igem.org/mediawiki/2017/8/83/ResultsPicture-SMS_Shenzhen.jpg)

# 1. Result of the Enzyme Activity Modeling Experiment

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1. Standard Calibration Curve for Bacterial Concentration:

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![]( https://static.igem.org/mediawiki/2017/5/55/EnzymeA3-SMS_shenzhen.jpg  )

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![]( https://static.igem.org/mediawiki/2017/5/5d/EnzymeA4-SMS_shenzhen.jpg  )

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2. Chitinase Activity Measurement

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![](  https://static.igem.org/mediawiki/2017/4/47/EnzymeA5-SMS_shenzhen.jpg )

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![]( https://static.igem.org/mediawiki/2017/a/a4/EnzymeA6-SMS_shenzhen.jpg  )
[[Click Here] to See More Details of This Experiment](https://2017.igem.org/Team%3ASMS_Shenzhen/Experiments/EnzymeActivity)

# 2. Result of sacB Promoter Test

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## Measurement of OD600

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![]( https://static.igem.org/mediawiki/2017/3/39/Zzsms-shenzhen.jpg )

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# Fluorescent Measurement

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![](https://static.igem.org/mediawiki/2017/e/ee/Ctb1.jpg )
![](https://static.igem.org/mediawiki/2017/2/26/Ctb2.jpg )
![](https://static.igem.org/mediawiki/2017/9/9c/Ctb3.jpg )

#

![]( https://static.igem.org/mediawiki/2017/e/e7/Ctb4.jpg  )
[[Click Here] to See More Details of This Experiment](https://2017.igem.org/Team%3ASMS_Shenzhen/Contribution)

# 3. Result of Electron Microscope Observation of Erosion of Scale Insect’s Shell after Introducing Protease

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![](  https://static.igem.org/mediawiki/2017/4/45/Expgroup1-SMS_Shenzhen.jpg )

Result Picture 1 - Experimental Group

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![](  https://static.igem.org/mediawiki/2017/f/f1/Control1-SMS_Shenzhen.jpg  )

Result Picture 2 - Control Group (without protease)

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The shell soaked in protease appears to be very different from that in the control group.

In control group, structure was relatively complete and the characteristics were clear, but the insect body of the experimental group had lost its structural characteristics under the action of protease, and the outer shell was severely eroded.

#

![]( https://static.igem.org/mediawiki/2017/1/11/Expgroup2-SMS_Shenzhen.jpg   )

Result Picture 3 - Experimental Group

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![](  https://static.igem.org/mediawiki/2017/2/20/Control2-SMS_Shenzhen.jpg  )

Result Picture 4 - Control Group (without protease)

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The sample in the experimental group (arrow marker) has obvious erosion marks compared to the control group, which is relatively complete and flat.

[[Click Here] to See More Details of This Experiment](https://2017.igem.org/Team%3ASMS_Shenzhen/Demonstrate)

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