iGEM 2017 — Team Bordeaux

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# Team:Bordeaux/Notebook

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* [HOME](https://2017.igem.org/Team%3ABordeaux)
* TEAM
  + [Meet us!](https://2017.igem.org/Team%3ABordeaux/Team)
  + [Collaborations](https://2017.igem.org/Team%3ABordeaux/Collaborations)

- PROJECT
  * [Description](https://2017.igem.org/Team%3ABordeaux/Description)
- LABWORK
  * [Experiments](https://2017.igem.org/Team%3ABordeaux/Experiments)
  * [Results](https://2017.igem.org/Team%3ABordeaux/Results)
  * [Notebook](https://2017.igem.org/Team%3ABordeaux/Notebook)
- [PARTS](https://2017.igem.org/Team%3ABordeaux/Parts)
- [BIOINFORMATICS](https://2017.igem.org/Team%3ABordeaux/Software)
- HUMAN PRACTICES
  * [Integrated Human Practices](https://2017.igem.org/Team%3ABordeaux/HP/Silver)
  * [Public Engagement](https://2017.igem.org/Team%3ABordeaux/Engagement)
- [ATTRIBUTIONS](https://2017.igem.org/Team%3ABordeaux/Attributions)

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Here, are presneted the Notebooks of our project !

Notebook | | || * [June](https://static.igem.org/mediawiki/2017/4/4b/T--Bordeaux--NotebookJune.pdf)   * [July](https://static.igem.org/mediawiki/2017/3/32/T--Bordeaux--NotebookJuly.pdf)   * [Août](https://static.igem.org/mediawiki/2017/2/26/T--Bordeaux--NotebookAout.pdf) | | |

# How to find us ?

Feel free to email us to provide some feedback on our project, have some information on the team and our work, or to just say hello !

[Facebook](https://www.facebook.com/BordeauxIGEM)
[Twitter](https://twitter.com/bordeauxigem)
[Wordpress](https://igembordeaux.wordpress.com/)

##### Mail: igembdx@gmail.com

##### *Copyright © iGEM Bordeaux 2017*

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# Team:Bordeaux/Experiments

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* [HOME](https://2017.igem.org/Team%3ABordeaux)
* TEAM
  + [Meet us!](https://2017.igem.org/Team%3ABordeaux/Team)
  + [Collaborations](https://2017.igem.org/Team%3ABordeaux/Collaborations)

- PROJECT
  * [Description](https://2017.igem.org/Team%3ABordeaux/Description)
- LABWORK
  * [Experiments](https://2017.igem.org/Team%3ABordeaux/Experiments)
  * [Results](https://2017.igem.org/Team%3ABordeaux/Results)
  * [Notebook](https://2017.igem.org/Team%3ABordeaux/Notebook)
- [PARTS](https://2017.igem.org/Team%3ABordeaux/Parts)
- [BIOINFORMATICS](https://2017.igem.org/Team%3ABordeaux/Software)
- HUMAN PRACTICES
  * [Integrated Human Practices](https://2017.igem.org/Team%3ABordeaux/HP/Silver)
  * [Public Engagement](https://2017.igem.org/Team%3ABordeaux/Engagement)
- [ATTRIBUTIONS](https://2017.igem.org/Team%3ABordeaux/Attributions)

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Protocols

## Preparation

* [Growing medium for *E. coli*](https://static.igem.org/mediawiki/2015/6/60/Growing_Medium_of_E.coli_VC.pdf)
* [PCR](https://static.igem.org/mediawiki/2015/b/b9/PCR_iGEM_Bordeaux_Protocol.pdf)
* [Plasmid extraction](https://static.igem.org/mediawiki/2015/5/5a/Miniprep_Camilla_V3_.pdf)

## How to work with *C. elegans*

* [Transformation and Microinjection in *C. elegans*](https://static.igem.org/mediawiki/2017/a/a2/Bordeaux2017_MicroInjection_WB.pdf)
* [Maintenance for *C. elegans*](https://static.igem.org/mediawiki/2017/8/8c/Bordeaux2017_Maintenance_WB.pdf)
* [**A**dvance **QU**ick **A**ssembly cloning](https://static.igem.org/mediawiki/2017/0/06/Bordeaux2017_Aquacloning.pdf)

# How to find us ?

Feel free to email us to provide some feedback on our project, have some information on the team and our work, or to just say hello !

[Facebook](https://www.facebook.com/BordeauxIGEM)
[Twitter](https://twitter.com/bordeauxigem)
[Wordpress](https://igembordeaux.wordpress.com/)

##### Mail: igembdx@gmail.com

##### *Copyright © iGEM Bordeaux 2017*

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Bordeaux/Results

![Wrong](https://img15.hostingpics.net/pics/396137font.png)

* [HOME](https://2017.igem.org/Team%3ABordeaux)
* TEAM
  + [Meet us!](https://2017.igem.org/Team%3ABordeaux/Team)
  + [Collaborations](https://2017.igem.org/Team%3ABordeaux/Collaborations)

- PROJECT
  * [Description](https://2017.igem.org/Team%3ABordeaux/Description)
- LABWORK
  * [Experiments](https://2017.igem.org/Team%3ABordeaux/Experiments)
  * [Results](https://2017.igem.org/Team%3ABordeaux/Results)
  * [Notebook](https://2017.igem.org/Team%3ABordeaux/Notebook)
- [PARTS](https://2017.igem.org/Team%3ABordeaux/Parts)
- [BIOINFORMATICS](https://2017.igem.org/Team%3ABordeaux/Software)
- HUMAN PRACTICES
  * [Integrated Human Practices](https://2017.igem.org/Team%3ABordeaux/HP/Silver)
  * [Public Engagement](https://2017.igem.org/Team%3ABordeaux/Engagement)
- [ATTRIBUTIONS](https://2017.igem.org/Team%3ABordeaux/Attributions)

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Results

## During our lab work, we managed to do 3 constructions:

pmyo2::GFP
pmyo3::unc-60::RFP
pmyo3::unc60::GFP
These 3 constructions xere microinjected in worms under the microscope.
Pmyo2::GFP is used as a brenchmark in order to know if our worms were well injected. The promoter pmyo2 is tissue specific, it allows the expression of the gene myo-2 in the pharynx of the C.elegans. Thanks to the green fluorescent pharynx, we can screen transformed worms from non transformed worms.
The two others constructions, pmyo3::unc-60::RFP and pmyo3::unc60::GFP, will allow us to see how the two different isoforms unc-60A (RFP) and unc-60B (GFP) are expressed in the body wall muscle.
Here are a few pictures about what we obtained under the microscope.

![feed worm GFP](https://static.igem.org/mediawiki/2017/f/f6/T--Bordeaux--celegansGFP.png)

Fed worm GFP

![feed worm GFP](https://static.igem.org/mediawiki/2017/8/8a/T--Bordeaux--celegansRFP.png)

Fed worm RFP

![starve worm GFP](https://static.igem.org/mediawiki/2017/f/f6/T--Bordeaux--celegansGFP.png)

Starving worm GFP

![starve worm RFP](https://static.igem.org/mediawiki/2017/5/5c/Bordeaux2017_CelegansStarve2RFP.jpeg)

Starving worm RFP

We observed the worms under two conditions: starved and non-starved worms. C.elegans eats the bacteria and fungi. When there are no more bacteria on the petri dish, the worms are starved. It can be useful to see if the starvation can change a phenotype, as the worm can be adaptive.

# How to find us ?

Feel free to email us to provide some feedback on our project, have some information on the team and our work, or to just say hello !

[Facebook](https://www.facebook.com/BordeauxIGEM)
[Twitter](https://twitter.com/bordeauxigem)
[Wordpress](https://igembordeaux.wordpress.com/)

##### Mail: igembdx@gmail.com

##### *Copyright © iGEM Bordeaux 2017*

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)