iGEM 2020 — Team Worldshaper-Shanghai

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# Team:Worldshaper-Shanghai/Notebook

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## Notebook

Notebook

### February

##### ·Work out a 2020 iGEM work schedule ·Professional theory learning ·Experimental skills training A 2020 iGEM work schedule was made for both dry team and wet team by team consultants. After that, all team members started online professional theory learning and virtual experimental skills training.

### March

##### ·Project brainstorming All team members together with our team consultants brainstormed for our team project. After thorough research considering project utility, research difficulty, and past experiences, Worldshaper-Shanghai 2020 decided to perform work related to prostate cancer and forged a primitive work plan for the year.

### April

##### ·iGEM competition rules learning ·Professional theory learning ·Brainstorming and discussion Team members learned iGEM competition rules from team consultants. Getting to know the rules, the team had several online meetings to discuss and brainstorm the final target and needed materials. At the same time, the online professional theory of synthetic biology learning was continued.

### May

##### ·Project final determination ·Background research The team further consolidated the project plan, focusing now on the diagnosis of prostate cancer, and formed a basic plan for wet teamwork. The group started more specific background research and opened up several social media accounts in order to increase visibility. Meanwhile, members continued the study of general professional theories of biology.

### June

##### Wet team： ·Background manuscript ·Safety form Dry team ： ·Team arrangement ·Wiki framework ·Questionnaire ·Public activities plan ·Expert interview plan ·Self-media update ·Team apparel/ Team flag Members were divided into wet team and dry team. Everyone was arranged by different jobs. After some researches, a background manuscript about prostate cancer and its diagnosis was written. Also, the team finished safety form of the experimental part. For wet teams, the Wiki framework is started to be built. Through the communications with experts, several plans for offline public activities and expert interview for prostate diagnosis were made. We updated our first self-media as well as the public questionnaire. Besides, art groups of the team designed team apparel and team flag.

### July

##### Due to the impact of the COVID-19 epidemic, our offline work was compressed in about a 3-week period (from 7/27 to 8/15) and focused on experiments. The first week was spent familiarizing with lab procedures and choosing primers; the second week was spent on extracting RNA from artificial urine, processing it, and performing PCRs; and the third week was spent performing in vitro transcription/translation, and testing fluorescence proteins. Wet team： ·Lab procedures coaching ·RNA extraction and primers choosing Dry team： ·Self-media update ·Questionnaire collection

##### On the first two days, the group watched professionals perform some of the basic experimental procedures, namely the use of pipettes, centrifugation, homogenization, PCR, and RNA extraction from cells. A plan on experiment proceedings was also formed. For the next three days, the group extracted the genome of prostate cancer cells and performed PCR using 6 pairs of primers to test for the specificity of the primers.

##### On the side of the dry team, we continued producing articles for our WeChat account and distributed relevant questionnaires in order to demonstrate the social need for our project.

### August

##### Wet team： ·Experiment ·Enrich RNA from samples ·RT ·PCR ·Toehold Switch ·Experimental record ·Analysis and arrangement of experimental results ·Applied design Dry team： ·Education ·Self-media update ·2020 technology exhibition in Zhejiang ·Collaboration ·With Dingshi ·Online meet up ·Expert interview ·Manuscript collection of human practice

##### For dry teams, early in August, we had a collaboration with another team called Dingshi. Through the collaboration, a game about toehold switch was created. Meanwhile, we interviewed more experts to gain further suggestions. At the end of August, members and team consultants attended the 2020 technology exhibition in Zhejiang of China to promote our project. After that, human practice manuscript was formed and collected.

##### On the wet team side, the lab part was continued and recorded until August 13th. August 3rd, part of members enriched RNA from 5 ml artificial urine samples under the guidance of the professor. August 4th, we practiced enriched RNA from samples in the morning, finished RT and PCR of yesterday, and transfer the competent cell with the plasmid in the afternoon. August 5th, we performed PCRs of yesterday. August 6th, we learned and done the plasmid extraction of Escherichia coli under guiding. August 7th to August 10th, in order to collect more data, we kept repeating enriching RNA from samples, doing RT, and running PCRs. August 11th, we did transcription and translation with toehold switch and tested cherry, the fluorescence proteins we chose. McSherry works well and shows color on our samples. August 12th and 13th, we repeated the progress of plasmid extraction of E.coli and stored for tomorrow. August 14th, the last day in our lab, we did transcription and translation with toehold switch again and double test with mcherry. The result shows color well. At last, we made an analysis of experimental results and checked the whole progress with professors. After finishing the lab part, our applied design was started to be written.

### September and October

##### -Wiki manuscript ·Modeling ·Applied design ·Promotion video ·Prepare for presentation ·Presentation video ·Poster ·Wiki web page optimization ·Model ·Final judging form ·Parts registry

##### With wet team offline work closed, the group is now focusing on writing necessary documents (description, project design, protocols, experiment results analysis, future aspiration, Part registration, applied design, science communication, human practice, and safety), a compilation of past writings, filming a promotion video, and filming the final presentation video. We plan to produce the first version of the documents in early September and mid-September, depending on the work needed and the nature of the documents. The final version of most of the documents is planned to be finished in late September. We spent the whole October completing and checking all deliverables for Final Visual Gaint Jamboree, such as wiki optimization, making presentation video and poster.

### November

##### The team gets ready for the online final presentation.

### Worldshaper-Shanghai 2020

New non-invasive technique for early stage prostate cancer diagnosis

Worldshaper-Shanghai 2020

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# Team:Worldshaper-Shanghai/Engineering

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## Engineering Success

Engineering Success

### 1. The selection of primers：

##### As mentioned in the project design, we got three pairs of primers for both KLK3 and PCA3. To improve the efficiency of PCR or RPA, we needed to choose the most appropriate pair of each gene. We did the PCR with the cDNA of prostate cancer cell PC3 and three pairs of primers, respectively. Based on the result of gel electrophoresis, we chose KLK3-2 382bp (KLK3-2F: TGGTGCTGCACCCCTCATC; KLK3-2R: GGGCAGGTCCATGACCTTC) and PCA3-2 196bp (PCA3-2F：GCAAGAGCCACAGAGGGAATG; PCA3-2R: GCGCACTCACCATGAAATGG) as the primer.

![results-figure-1](https://static.igem.org/mediawiki/2020/b/bf/T--Worldshaper-Shanghai--results-1.png)

###### Figure1. Primer test for both KLK3 and PCA3

### 2. The test of overhang-added primers:

##### We added a T7 sequence at the 5’ end of forward primer to help start the process of transcription. For the reverse primer, we designed it with a trigger at the 5’ end, so as to add the trigger sequence to the end of PCR product during the process of PCR. The sequences of these overhang-added primers are as follow (The green part is the T7 overhang, while the orange part is the trigger sequence): T-KLK3-2F：taatacgactcactatagggTGGTGCTGCACCCCTCATC T-KLK3-2R: gtttgaatgaattgtaggcttgttatagttatgtttGGGCAGGTCCATGACCTTC T-PCA3-2F: taatacgactcactatagggGCAAGAGCCACAGAGGGAATG T-PCA3-2R:gtttgaatgaattgtaggcttgttatagttatgtttGCGCACTCACCATGAAATGG The results of PCR showed that the primers with overhang could also amplify KLK3 and PCA3 successfully without significantly affecting the amplification efficiency.

![results-figure-2](https://static.igem.org/mediawiki/2020/8/8d/T--Worldshaper-Shanghai--results-2.png)

###### Figure2. PCR efficiency test of two kinds of overhang-added primers

### 3.The preparation for toehold switch:

##### Since RNA is a kind of unstable nucleic acid that difficult to be preserved, we constructed T7 promoter and toehold switch sequence as well as mCherry into pSB1C3 backbone (we called it “toehold plasmids” later), which could be transcripted to toehold switch RNA by in vitro transcription. The synthesized toehold-switch plasmids were transformed into E.coli for further culture in LB liquid culture with ampicillin, the new single colonies were verified by DNA sequencing: The sequencing report of these PCR identified colonies showed that the toehold sequence is proper without the base mutation (Figure 3). Finally, we extracted plasmids from these colonies for future use.

![results-figure-3](https://static.igem.org/mediawiki/2020/2/25/T--Worldshaper-Shanghai--results-3.png)

###### Figure 3. The sequencing report of Toehold swich-mCherry

### 4. In vitro transcription/translation

##### The last step involved in the prostate cancer testing procedure is in vitro transcription/translation, which involves transcribing the PCR products and the toehold plasmids into RNA, and then the trigger RNA sequence on PCR product starts the translation of the red fluorescence protein (mCherry) that on the downstream of toehold switch sequence. The whole reaction is based on S30 T7 High-Yield Protein Expression System from Promega, the PCR products of PCA3 (we called it “PCA3 products” later) were selected for validation during this step. The results were as anticipated: the control group 1 without toehold plasmids showed no sign of red fluorescence; the control group 2 without PCA3 products showed faint fluorescence, and the samples containing PCA3 products and toehold plasmids showed brilliant fluorescence (Figure 4).

![results-figure-4](https://static.igem.org/mediawiki/2020/8/82/T--Worldshaper-Shanghai--results-4.png)

###### Figure 4. The verification of in vitro transcription/translation involving PCA3 products and toehold plasmids \*Control 1: Toehold plasmids free; Control 2: PCA3 products free; Sample1/2: Samples containing PCA3 products and toehold plasmids.

### 5. Product simulation and verification

##### After choosing the primer and verifying the efficiency of overhang-added primers and in vitro transcription/translation process, we use synthetic urine (CAS Number: R23032-500ml, Shanghai yuan ye Bio-Technology Co., Ltd) to simulate the usage of our product. PCA3 mRNA was added into synthetic urine in advance. A standard curve for the detection of PCA3 in synthetic urine is plotted to determine the effective interval of copies of PCA3 in urine for the detection (Figure5 a), the fluorescence value increased linearly in the range of 200-800 copies, which can be used as an effective range to identify the content of PCA3 mRNA in synthetic urine. For evaluating the reliability of our products, we developed a receiver operating characteristic curve (ROC curve). The amount of PCA3 mRNA is 300 copies of PCA3 mRNA in urine samples. The AUC (area under the curve) value is calculated to be 82.4%, which preliminarily proved the effectiveness of our diagnosis (Figure5 b). More details in [Protocol](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Protocols)，[Proof of Concept](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Proof_Of_Concept)，[Model](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Model) page.

![results-figure-5](https://static.igem.org/mediawiki/2020/7/73/T--Worldshaper-Shanghai--results-5.png)

###### Figure 5. The Standard Curve and ROC Curve for product evaluating

### 6.Future Work

##### Using an accurate genetic-based test to select appropriate candidates for prostate biopsies can limit the number of unnecessary biopsies performed and save costs to payers and to the national healthcare system[1]. In order to improve our product design and put forward a better evaluation standard combined with the use of the kit, the following improvements need to be done in this project.

#### 6.1 RPA testing

##### In order to accommodate the product for a broader range of circumstances, and to minimize the restriction set by equipment and environmental requirements, our project, a urine-based non-invasive diagnosis for early prostate cancer, decide to adopt the RPA method in replacement of PCR reaction, because RPA methods, under identical temperature, enables a shorter time requirement for gene amplification. We weren’t able to complete this part of the experiment because of the impact of COVID-19, so our first priority in the future will be testing for the ideal reaction time and temperature of both PCA3 and KLK3. It has been discovered that there are certain defects regarding the RPA method, including the primer-dimer and non-specific amplification that are liable to occur in the process[2]. To a certain extent, testing for a more precise reaction time and temperature will prevent the occurrence of non-specific amplification and primer-dimer. Furthermore, since KLK3 already has a relatively higher expression in healthy people than PCA3, we will have to adjust some of the experimental parameters to prevent a false positive test due to the over-amplification of the gene.

#### 6.2 To further improve the applied design

##### As of now, we have already incorporated the RPA method into the preliminary design, and we will further improve the preliminary design after conducting experiments with the RPA method, making our product more practical and effective. The applied design we proposed this season has been uploaded to the [Proposed Implementation](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Implementation)Proposed Implementation page.

#### 6.3 Sensitivity and reliability test on the kit

##### Basing on this year’s work, our current experiment method has successfully achieved the goal of detecting PCA3 molecule in synthetic urine samples. Not only did we successfully run the experiment, we further elaborated on the experiment by producing both a standard curve and a ROC curve that describes both the effective interval for detection and accuracy. Another test should be run on the above indexes of PCA3 and KLK3 on the synthetic urine after incorporating the RPA method into our experiment.

#### 6.4 Test with actual human urines

##### Running the experiment with both urine of healthy people and prostate cancer patients, complying with the consent of patients and legal regulation of the country, will help us establish a method of evaluation for prostate cancer diagnosis by cutoff value. A possible way has been found:There is a probe-based kit available on the market called Progensa PCA3 diagnosis (Gen-Probe, SanDiego, CA, USA). The evaluation method of the kit for prostate cancer, PCA3 ratio, provides a good reference for us. PCA3 ratio, also called the PCA3 score, is much more cancer-specific than serum PSA levels, which are confounded by factors such as prostate volume, age, trauma, and certain drugs. PCA3 score is the ratio of mRNA transcripts of PCA3 to KLK3 in urine (PCA3 score= PCA3mRNA/PSA mRNA×1000), which can be the numerical evidence to determine prostate cancer [3-5].Reference[1] Aubry W, Lieberthal R, Willis A, et al. Budget Impact Model: Epigenetic Assay Can Help Avoid Unnecessary Repeated Prostate Biopsies and Reduce Healthcare Spending[J]. American Health and Drug Benefits, 2013, 6(1):15-24. [2]Lobato, Ivan Magriñá, O’ Sullivan, Ciara K. Recombinase polymerase amplification: Basics, applications, and recent advances[J]. Trac Trends in Analytical Chemistry, 2018:S0165993617302583 [3] Deras IL, Aubin SM, Blase A, et al. PCA3: a molecular urine assay for predicting prostate biopsy outcome. J Urol. 2008;179:1587–1592. [4]Durand X, Moutereau S, Xylinas E, et al. ProgensaTM PCA3 test for prostate cancer[J]. Expert Review of Molecular Diagnostics, 2011, 11(2):137-144. [5]Marks L S, Bostwick D G . Prostate Cancer Specificity of PCA3 Gene Testing: Examples from Clinical Practice.[J]. Reviews in urology, 2008, 10(3):175-181.

### Worldshaper-Shanghai 2020

New non-invasive technique for early stage prostate cancer diagnosis

Worldshaper-Shanghai 2020

### Quick Link

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* [Parts](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Parts)
* [Human Practice](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

### Contact

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# Team:Worldshaper-Shanghai/Experiments

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### Worldshaper-Shanghai 2020

## Black lives Matter

### May 31 - November, 2020.

## Worldshaper-Shanghai

### Worldshaper-Shanghai 2020

## New non-invasive technique for early stage prostate cancer diagnosis

What’s Going on

### Latest Research on Prostate Cancer

![](https://static.igem.org/mediawiki/2020/3/36/T--Worldshaper-Shanghai--collection-3-1.jpg)

### Wet Lab 7.17

![](https://static.igem.org/mediawiki/2020/5/55/T--Worldshaper-Shanghai--collection-3-2.jpg)

### Wet Lab 7.20

![](https://static.igem.org/mediawiki/2020/8/80/T--Worldshaper-Shanghai--collection-3-3.jpg)

### Wet Lab 7.25

![](https://static.igem.org/mediawiki/2020/3/36/T--Worldshaper-Shanghai--collection-3-1.jpg)

### Wei Lab 7.27

![](https://static.igem.org/mediawiki/2020/5/55/T--Worldshaper-Shanghai--collection-3-2.jpg)

### Wet Lab 7.28

![](https://static.igem.org/mediawiki/2020/8/80/T--Worldshaper-Shanghai--collection-3-3.jpg)

### Wet Lab 7.31

![](https://static.igem.org/mediawiki/2020/3/36/T--Worldshaper-Shanghai--collection-3-1.jpg)

### Wet Lab 8.3

![](https://static.igem.org/mediawiki/2020/5/55/T--Worldshaper-Shanghai--collection-3-2.jpg)

### Wet Lab 8.4

![](https://static.igem.org/mediawiki/2020/8/80/T--Worldshaper-Shanghai--collection-3-3.jpg)

### Wet Lab 8.7

![](https://static.igem.org/mediawiki/2020/a/aa/T--Worldshaper-Shanghai--about-3-1.jpg)

About Worldshaper-Shanghai

### Who Are We？

Team introduction

The most common clinical diagnosis nowadays for prostate cancer is digital rectal examination，serum PSA test and prostate biopsy, which, to some extend, is invasive or can cause physical discomfort. The goal of Worldshaper-Shanghai 2020 is to develop a new non-invasive technique for early stage prostate cancer diagnosis, mainly by expressing the color variation of the tested urine since urine test could be the simplest, painless and rapid test method for prostate cancer diagnosis.Our equipment design combined the technology of reverse transcription, gene amplification (PCR/RPA) and RNA hairpin structure (Toehold switch), to detect the specificity of RNA biomarkers of prostate cancer in the urine (PCA3 and KLK3), the final red fluorescence protein output will be report as visible results, so as to achieve the purpose of our visual diagnosis of prostate cancer.

Worldshaper-Shanghai 2020

### See the Human Practice

Human practice introduction

See all Human Practice

![](https://static.igem.org/mediawiki/2020/8/89/T--Worldshaper-Shanghai--collection-4-1.jpg)

### Interview In Changhai Hospital

![](https://static.igem.org/mediawiki/2020/5/5b/T--Worldshaper-Shanghai--collection-4-2.jpg)

### Group Activity In Shanghai

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### Group Acitivity In Shanghai

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### Education In Hangzhou

![](https://static.igem.org/mediawiki/2020/a/a5/T--Worldshaper-Shanghai--collection-4-5.jpg)

### Education In Hangzhou

![](https://static.igem.org/mediawiki/2020/a/a4/T--Worldshaper-Shanghai--collection-4-6.jpg)

### Education In Shanghai JiaoTong University

What’s Going on

### Our Project

Project
Description

![](https://static.igem.org/mediawiki/2020/e/e9/T--Worldshaper-Shanghai--event-1-1.jpg)

### [Worldshaper Shanghai](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Description)

2020

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Design
Overview

![](https://static.igem.org/mediawiki/2020/1/12/T--Worldshaper-Shanghai--event-1-5.jpg)

### [Worldshaper Shanghai](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Design)

2020

[Learn More](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Design)

Proposed
Implementation

![](https://static.igem.org/mediawiki/2020/f/f1/T--Worldshaper-Shanghai--event-1-3.jpg)

### [Worldshaper Shanghai](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Design)

2020

[Learn More](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Design)

![](https://static.igem.org/mediawiki/2020/3/3d/T--Worldshaper-Shanghai--brand-1-1.png)

![](https://static.igem.org/mediawiki/2020/b/b3/T--Worldshaper-Shanghai--brand-1-2.png)

![](https://static.igem.org/mediawiki/2020/f/fd/T--Worldshaper-Shanghai--brand-1-3.png)

![](https://static.igem.org/mediawiki/2020/3/37/T--Worldshaper-Shanghai--brand-1-4.png)

![](https://static.igem.org/mediawiki/2020/8/8a/T--Worldshaper-Shanghai--brand-1-5.png)

![](https://static.igem.org/mediawiki/2020/3/3d/T--Worldshaper-Shanghai--brand-1-1.png)

![](https://static.igem.org/mediawiki/2020/b/b3/T--Worldshaper-Shanghai--brand-1-2.png)

![](https://static.igem.org/mediawiki/2020/f/fd/T--Worldshaper-Shanghai--brand-1-3.png)

![](https://static.igem.org/mediawiki/2020/3/37/T--Worldshaper-Shanghai--brand-1-4.png)

![](https://static.igem.org/mediawiki/2020/8/8a/T--Worldshaper-Shanghai--brand-1-5.png)

Our Team

### Core Members

![](https://static.igem.org/mediawiki/2020/5/56/T--Worldshaper-Shanghai--team-1-1.jpeg)

### Marnie Wang

Captain, Wet Lab,Art Design,Human Practice

![](https://static.igem.org/mediawiki/2020/3/33/T--Worldshaper-Shanghai--team-1-2.jpeg)

### Bob Zhao

Wet Lab,Model building,Human Practice

![](https://static.igem.org/mediawiki/2020/e/e3/T--Worldshaper-Shanghai--team-1-3.jpeg)

### Cheryl Guo

Wet lab Captain

Worldshaper News

### Latest From Our News

![](https://static.igem.org/mediawiki/2020/8/8b/T--Worldshaper-Shanghai--blog-1-1.jpeg)

8.17, 2020

* Film by
* Bob Zhao

### [Interview](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

Human Practice

[Learn More](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

![](https://static.igem.org/mediawiki/2020/f/ff/T--Worldshaper-Shanghai--blog-1-2.png)

8.17, 2020

* Film by
* Marnie Wang

### [Interview](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

Human Practice

[Learn More](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

![](https://static.igem.org/mediawiki/2020/e/ec/T--Worldshaper-Shanghai--blog-1-3.jpeg)

8.17, 2020

* Film by
* Cheryl Guo

### [Interview](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

Human Practice

[Learn More](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

### Worldshaper-Shanghai 2020

New non-invasive technique for early stage prostate cancer diagnosis

Worldshaper-Shanghai 2020

### Quick Link

* [Team](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Team)
* [Experiment](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Engineering)
* [Parts](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Parts)
* [Human Practice](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

### Contact

[worldshapershanghai2020@outlook.com](worldshapershanghai2020%40outlook.com)

© Copyright 2020 Worldshaper-Shanghai. All Rights Reserved

[![](https://static.igem.org/mediawiki/2020/7/7b/T--Worldshaper-Shanghai--logo-footer-1.png)](index.html)

Terms & conditions
Privacy policy & Terms of use

[![](https://static.igem.org/mediawiki/2020/1/1e/T--Worldshaper-Shanghai--logo-light-1-1.png)](https://2020.igem.org/Team%3AWorldshaper-Shanghai)

(c) 2020 Worldshaper-Shanghai 2020 - All rights reserved.

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:Worldshaper-Shanghai/Results

Home|| Worldshaper-Shanghai 2020 || Worldshaper-Shanghai HTML Template

[![](https://static.igem.org/mediawiki/2020/1/1e/T--Worldshaper-Shanghai--logo-light-1-1.png)](https://2020.igem.org/Team%3AWorldshaper-Shanghai)

* [Home](https://2020.igem.org/Team%3AWorldshaper-Shanghai)* [Team](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Team)
    + [Attribution](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Attributions)
    + [Team members](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Team)
  * [Model](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Model)
  * [Parts](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Parts)
  * Project
    + [Project Description](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Description)
    + [Design](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Design)
    + [Proposed Implementation](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Implementation)
  * [Human Practice](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)
    + [Integrated Human Pra-ctices](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)
    + [Science Communicati-on](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Education)
    + [Collaborations](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Collaborations)
    + [Partnership](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Partnership)
  * [Experiment](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Engineering)
    + [Engineering Success](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Engineering)
    + [Contribution](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Contribution)
    + [Proof of concept](https://2020.igem.org/Team%3ATeam%3AWorldshaper-Shanghai/Proof_Of_Concept)
    + [Notebook](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Notebook)
    + [Protocols](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Protocol)
    + [Safety](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Safety)
  [Judging Form](https://igem.org/2020_Judging_Form?id=3577)

### Worldshaper-Shanghai 2020

## Black lives Matter

### May 31 - November, 2020.

## Worldshaper-Shanghai

### Worldshaper-Shanghai 2020

## New non-invasive technique for early stage prostate cancer diagnosis

What’s Going on

### Latest Research on Prostate Cancer

![](https://static.igem.org/mediawiki/2020/3/36/T--Worldshaper-Shanghai--collection-3-1.jpg)

### Wet Lab 7.17

![](https://static.igem.org/mediawiki/2020/5/55/T--Worldshaper-Shanghai--collection-3-2.jpg)

### Wet Lab 7.20

![](https://static.igem.org/mediawiki/2020/8/80/T--Worldshaper-Shanghai--collection-3-3.jpg)

### Wet Lab 7.25

![](https://static.igem.org/mediawiki/2020/3/36/T--Worldshaper-Shanghai--collection-3-1.jpg)

### Wei Lab 7.27

![](https://static.igem.org/mediawiki/2020/5/55/T--Worldshaper-Shanghai--collection-3-2.jpg)

### Wet Lab 7.28

![](https://static.igem.org/mediawiki/2020/8/80/T--Worldshaper-Shanghai--collection-3-3.jpg)

### Wet Lab 7.31

![](https://static.igem.org/mediawiki/2020/3/36/T--Worldshaper-Shanghai--collection-3-1.jpg)

### Wet Lab 8.3

![](https://static.igem.org/mediawiki/2020/5/55/T--Worldshaper-Shanghai--collection-3-2.jpg)

### Wet Lab 8.4

![](https://static.igem.org/mediawiki/2020/8/80/T--Worldshaper-Shanghai--collection-3-3.jpg)

### Wet Lab 8.7

![](https://static.igem.org/mediawiki/2020/a/aa/T--Worldshaper-Shanghai--about-3-1.jpg)

About Worldshaper-Shanghai

### Who Are We？

Team introduction

The most common clinical diagnosis nowadays for prostate cancer is digital rectal examination，serum PSA test and prostate biopsy, which, to some extend, is invasive or can cause physical discomfort. The goal of Worldshaper-Shanghai 2020 is to develop a new non-invasive technique for early stage prostate cancer diagnosis, mainly by expressing the color variation of the tested urine since urine test could be the simplest, painless and rapid test method for prostate cancer diagnosis.Our equipment design combined the technology of reverse transcription, gene amplification (PCR/RPA) and RNA hairpin structure (Toehold switch), to detect the specificity of RNA biomarkers of prostate cancer in the urine (PCA3 and KLK3), the final red fluorescence protein output will be report as visible results, so as to achieve the purpose of our visual diagnosis of prostate cancer.

Worldshaper-Shanghai 2020

### See the Human Practice

Human practice introduction

See all Human Practice

![](https://static.igem.org/mediawiki/2020/8/89/T--Worldshaper-Shanghai--collection-4-1.jpg)

### Interview In Changhai Hospital

![](https://static.igem.org/mediawiki/2020/5/5b/T--Worldshaper-Shanghai--collection-4-2.jpg)

### Group Activity In Shanghai

![](https://static.igem.org/mediawiki/2020/0/04/T--Worldshaper-Shanghai--collection-4-3.jpg)

### Group Acitivity In Shanghai

![](https://static.igem.org/mediawiki/2020/8/88/T--Worldshaper-Shanghai--collection-4-4.jpg)

### Education In Hangzhou

![](https://static.igem.org/mediawiki/2020/a/a5/T--Worldshaper-Shanghai--collection-4-5.jpg)

### Education In Hangzhou

![](https://static.igem.org/mediawiki/2020/a/a4/T--Worldshaper-Shanghai--collection-4-6.jpg)

### Education In Shanghai JiaoTong University

What’s Going on

### Our Project

Project
Description

![](https://static.igem.org/mediawiki/2020/e/e9/T--Worldshaper-Shanghai--event-1-1.jpg)

### [Worldshaper Shanghai](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Description)

2020

[Learn More](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Description)

Design
Overview

![](https://static.igem.org/mediawiki/2020/1/12/T--Worldshaper-Shanghai--event-1-5.jpg)

### [Worldshaper Shanghai](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Design)

2020

[Learn More](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Design)

Proposed
Implementation

![](https://static.igem.org/mediawiki/2020/f/f1/T--Worldshaper-Shanghai--event-1-3.jpg)

### [Worldshaper Shanghai](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Design)

2020

[Learn More](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Design)

![](https://static.igem.org/mediawiki/2020/3/3d/T--Worldshaper-Shanghai--brand-1-1.png)

![](https://static.igem.org/mediawiki/2020/b/b3/T--Worldshaper-Shanghai--brand-1-2.png)

![](https://static.igem.org/mediawiki/2020/f/fd/T--Worldshaper-Shanghai--brand-1-3.png)

![](https://static.igem.org/mediawiki/2020/3/37/T--Worldshaper-Shanghai--brand-1-4.png)

![](https://static.igem.org/mediawiki/2020/8/8a/T--Worldshaper-Shanghai--brand-1-5.png)

![](https://static.igem.org/mediawiki/2020/3/3d/T--Worldshaper-Shanghai--brand-1-1.png)

![](https://static.igem.org/mediawiki/2020/b/b3/T--Worldshaper-Shanghai--brand-1-2.png)

![](https://static.igem.org/mediawiki/2020/f/fd/T--Worldshaper-Shanghai--brand-1-3.png)

![](https://static.igem.org/mediawiki/2020/3/37/T--Worldshaper-Shanghai--brand-1-4.png)

![](https://static.igem.org/mediawiki/2020/8/8a/T--Worldshaper-Shanghai--brand-1-5.png)

Our Team

### Core Members

![](https://static.igem.org/mediawiki/2020/5/56/T--Worldshaper-Shanghai--team-1-1.jpeg)

### Marnie Wang

Captain, Wet Lab,Art Design,Human Practice

![](https://static.igem.org/mediawiki/2020/3/33/T--Worldshaper-Shanghai--team-1-2.jpeg)

### Bob Zhao

Wet Lab,Model building,Human Practice

![](https://static.igem.org/mediawiki/2020/e/e3/T--Worldshaper-Shanghai--team-1-3.jpeg)

### Cheryl Guo

Wet lab Captain

Worldshaper News

### Latest From Our News

![](https://static.igem.org/mediawiki/2020/8/8b/T--Worldshaper-Shanghai--blog-1-1.jpeg)

8.17, 2020

* Film by
* Bob Zhao

### [Interview](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

Human Practice

[Learn More](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

![](https://static.igem.org/mediawiki/2020/f/ff/T--Worldshaper-Shanghai--blog-1-2.png)

8.17, 2020

* Film by
* Marnie Wang

### [Interview](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

Human Practice

[Learn More](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

![](https://static.igem.org/mediawiki/2020/e/ec/T--Worldshaper-Shanghai--blog-1-3.jpeg)

8.17, 2020

* Film by
* Cheryl Guo

### [Interview](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

Human Practice

[Learn More](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

### Worldshaper-Shanghai 2020

New non-invasive technique for early stage prostate cancer diagnosis

Worldshaper-Shanghai 2020

### Quick Link

* [Team](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Team)
* [Experiment](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Engineering)
* [Parts](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Parts)
* [Human Practice](https://2020.igem.org/Team%3AWorldshaper-Shanghai/Human_Practices)

### Contact

[worldshapershanghai2020@outlook.com](worldshapershanghai2020%40outlook.com)

© Copyright 2020 Worldshaper-Shanghai. All Rights Reserved

[![](https://static.igem.org/mediawiki/2020/7/7b/T--Worldshaper-Shanghai--logo-footer-1.png)](index.html)

Terms & conditions
Privacy policy & Terms of use

[![](https://static.igem.org/mediawiki/2020/1/1e/T--Worldshaper-Shanghai--logo-light-1-1.png)](https://2020.igem.org/Team%3AWorldshaper-Shanghai)

(c) 2020 Worldshaper-Shanghai 2020 - All rights reserved.

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)