iGEM 2018 — Team FJNU-China

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# Team:FJNU-China/Notebook

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[HOME](https://2018.igem.org/Team%3AFJNU-China)

TEAM

[Team Members](https://2018.igem.org/Team%3AFJNU-China/Team)
[Collaborations](https://2018.igem.org/Team%3AFJNU-China/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AFJNU-China/Description)
[Design](https://2018.igem.org/Team%3AFJNU-China/Design)
[Experiments](https://2018.igem.org/Team%3AFJNU-China/Experiments)
[Notebook](https://2018.igem.org/Team%3AFJNU-China/Notebook)
[InterLab](https://2018.igem.org/Team%3AFJNU-China/InterLab)
[Model](https://2018.igem.org/Team%3AFJNU-China/Model)
[Results](https://2018.igem.org/Team%3AFJNU-China/Results)
[Demonstrate](https://2018.igem.org/Team%3AFJNU-China/Demonstrate)
[Improve](https://2018.igem.org/Team%3AFJNU-China/Improve)
[Attributions](https://2018.igem.org/Team%3AFJNU-China/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AFJNU-China/Parts)
[Basic Parts](https://2018.igem.org/Team%3AFJNU-China/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AFJNU-China/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AFJNU-China/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AFJNU-China/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AFJNU-China/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AFJNU-China/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AFJNU-China/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AFJNU-China/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AFJNU-China/Hardware)
[Measurement](https://2018.igem.org/Team%3AFJNU-China/Measurement)
[Model](https://2018.igem.org/Team%3AFJNU-China/Model)
[Plant](https://2018.igem.org/Team%3AFJNU-China/Plant)
[Software](https://2018.igem.org/Team%3AFJNU-China/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=FJNU-China)

[![FJNU-China ](https://static.igem.org/mediawiki/2018/9/9d/T--FJNU-China--index_logo.png)](https://2018.igem.org/Team%3AFJNU-China)

* [Home](https://2018.igem.org/Team%3AFJNU-China)
* Team
  + [About us](https://2018.igem.org/Team%3AFJNU-China/Team)
  + [Attributions](https://2018.igem.org/Team%3AFJNU-China/Attributions)
  + [Gallery](https://2018.igem.org/Team%3AFJNU-China/Gallery)
* Projects
  + [Description](https://2018.igem.org/Team%3AFJNU-China/Description)
  + [Design](https://2018.igem.org/Team%3AFJNU-China/Design)
  + [Results](https://2018.igem.org/Team%3AFJNU-China/Result)
  + [Application](https://2018.igem.org/Team%3AFJNU-China/Applied_Design)
  + [Demonstrate](https://2018.igem.org/Team%3AFJNU-China/Demonstrate)
* [Model](https://2018.igem.org/Team%3AFJNU-China/Model)
* Laboratory
  + [Notebook](https://2018.igem.org/Team%3AFJNU-China/Notebook)
  + [InterLab](https://2018.igem.org/Team%3AFJNU-China/InterLab)
  + [Safety](https://2018.igem.org/Team%3AFJNU-China/Safety)
* Parts
  + [Basic Part](https://2018.igem.org/Team%3AFJNU-China/Basic_Part)
  + [Composite Part](https://2018.igem.org/Team%3AFJNU-China/Composite_Part)
  + [Improve](https://2018.igem.org/Team%3AFJNU-China/Improve)
  + [Part List](https://2018.igem.org/Team%3AFJNU-China/Parts)
* HP
  + [Overview](https://2018.igem.org/Team%3AFJNU-China/Overview)
  + [Intergrated HP](https://2018.igem.org/Team%3AFJNU-China/Human_Practices)
  + [Engagement&
    Public Education](https://2018.igem.org/Team%3AFJNU-China/Public_Engagement)
  + [Collaborations](https://2018.igem.org/Team%3AFJNU-China/Collaborations)
* [Medals](https://2018.igem.org/Team%3AFJNU-China/Medals)

# An unforgettable journey

## Week1    27/10/2017

First meeting, Team FJNU-China was established.

## Week2    03/2-10/2

Learn the molecular biology experimental techniques under the guidance of Dr.Yang.

## Week3-Week7    Apr

Sent d-ldh, Tyrb and rocG to the company for synthesis.[Obtained d-ldh, Tyrb and rocG fragment by PCR.[Result1]](https://static.igem.org/mediawiki/2018/1/17/T--FJNU-China--PCR_Tyrb.png) [[Result2]](https://static.igem.org/mediawiki/2018/b/ba/T--FJNU-China--pcr_D-ldh.jpg)[[Result3]](https://static.igem.org/mediawiki/2018/8/81/T--FJNU-China--Fig.8_3.1_rocG_pcr_KZ_.jpg)

## Week8-Week12    May

1.Extract genome of E. coli Bw25113.2.Construct d-ldh, Tyrb and rocG by Gibson assembly.
3.PCR verification.
[[Result]](https://static.igem.org/mediawiki/2018/3/3d/T--FJNU-China--Fig.8_3.2_rocG_pcr_YZ_.jpg)

## Week13-Week17    June

1.Extracted plasmid of E. coli BM4R
2.Cultured Clostridium acetobutylicum
3.Induction culture of engineering bacteria
4.SDS-PAGE verifies protein expression
[[Result]](https://static.igem.org/mediawiki/2018/7/72/T--FJNU-China--SDS-PAGE_Dldh-Tryb-rocG.jpg)

## Week18    1/7-7/7

1.Synthetic primer for our experiments.
2.Cultured staphylococcus epidermidis.
3.Did a PCR pre-experiment to amplify atoD, atoA
4.Verified that if E.coli can grow without salt.
5.Held a summer camp with Quanzhou 7th High school and Fuzhou 8th High school.

## Week19    8/7-14/7

1.Made 16s PCR of Staphylococcus epidermidis.[[Result]](https://static.igem.org/mediawiki/2018/d/db/T--FJNU-China--16spcr.png)
2.Did the lethal experiment of PLA against Staphylococcus epidermidis.
3.Knocked out Kana resistance of TnaA-/- deletion strain.
4.Amplified atoD and atoA [[Result]](https://static.igem.org/mediawiki/2018/5/5d/T--FJNU-China--atoD-atoA.png)
5.InterLab

## Week21    15/7-21/7

1.Did the lethal experiment of PLA against Staphylococcus epidermidis.
2.Obtained the MazF toxin protein gene fragment from E.coli BW25113
3.The MazF and PEA32 backbones were connection by Gibsion. [[Result]](https://static.igem.org/mediawiki/2018/4/42/T--FJNU-China--mazF-backbone.png)
4.Obtained lacI fragment by PCR. [[Result]](https://static.igem.org/mediawiki/2018/5/5e/T--FJNU-China--PCR-lacI.png)
5.InterLab

## Week22    22/7-28/7

1.Attended the iGEM Eurasian Meetup 2018
2.Verified the function of MazF with different concentrations of arabinose concentration.[[Result]](https://static.igem.org/mediawiki/2018/5/57/T--FJNU-China--arabinose-induced-MazF.png)
3.Did the T4 ligation for lacI and the circuit we synthesized.
4.Added different concentrations of PLA to samples and Measured OD value.
5.Tried to obtain eGFP fragment by PCR.
6.Uploaded the data of InterLab.

## Week23    29/7-04/8

1.Making a standard curve of PLA.
2.Failed to constructed the circuit of hns-syn-eGFP, we decided to replace the eGFP with the GFP.
3.Change the backbone of the plasmid hns-syn from PUC57 into pRB1S.
4.Add restriction sites for atoD, atoA by PCR.
5.Did the lethal experiment of PLA against Staphylococcus epidermidis to provide data for our model.

## Week24    5/8-11/8

1.Failed to enzyme cutting pRB1s, PLA and mazF with Kpn1 and Dra3.
2.Failed to construct pRB1s-atoD-atoA by Gibson assembly.
3.Obtained GFP fragment by PCR.
4.Tried to construct pRB1s-hns-syn-GFP.
5.Obtained ARO10, par and TyrB fragment by PCR.
[[Result]](https://2018.igem.org/File%3AT--FJNU-China--ARO10-par-TyrB.jpg)
6. Construct ARO10, par, TyrB and pSB1C3 by Gibson assembly.

## Week25    12/8-18/8

1.Constructed pSB1C3-atoD and pSB1C3-atoA by T4 connection.
2.Communicated with XMU-China.
3.The lethal experiment of mazf against Staphylococcus epidermidis.
4.Site-directed mutation.

## Week26    19/8-25/8

1.The lethal experiment of mazf against Staphylococcus epidermidis.
2.T4 ligation for GFP and PLA.
3.Measured the production of 2-PE and PLA

## Week27    26/8-01/9

Attended 5th ccic in Shanghai,China.

## Week28    2/9-8/9

1.Got the fragment of adhE2.
2.Cultured our engineered bacteria in 0M, 0.05M, and 0.3M at 25℃ and 37℃.

## Week29    9/9-15/9

1.Construct pRB1s-atoD-atoA-adhE2 and composite part. [[Result]](https://static.igem.org/mediawiki/2018/8/89/T--FJNU-China--atoa-atod-adhe2.jpg)
2.T4 ligation for salt-temperature circuit and pSB1C3.
3.Line cultured the hns-syn on the different salt concentration of plates.

## Week30    16/9-22/9

1.Observed the GFP level under fluorescence microscope.[[Result]](https://static.igem.org/mediawiki/2018/5/54/T--FJNU-China--fluorescence-microscope.png)
2.verify whether circuit is successfully constructed.
3.Construct improve part.

## Week31    23/9-29/9

Constructed araC+pBAD+tetR+GFP+PLtetO1+RFP part. [[Result]](https://static.igem.org/mediawiki/2018/d/db/T--FJNU-China--tetrteto.png)

## Week29    30/9-07/10

1.Submitted our parts
2.Made wiki
3.Verified circuit function

## Lab Endding! See our Lab book and protocol here!

#

### Contact Us

E-mail:  igem2018fjnu@gmail.com

### Attribution

[![](https://static.igem.org/mediawiki/2018/1/17/T--FJNU-China--index_life.png)](http://life.fjnu.edu.cn/)
[![](https://static.igem.org/mediawiki/2018/e/ec/T--FJNU-China--index_taoyong.png)](http://www.im.cas.cn/)
![](https://static.igem.org/mediawiki/2018/0/0d/T--FJNU-China--index_biolab.png)
![](https://static.igem.org/mediawiki/2018/2/2a/T--FJNU-China--index_Dobio.png)


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# Team:FJNU-China/Experiments

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[HOME](https://2018.igem.org/Team%3AFJNU-China)

TEAM

[Team Members](https://2018.igem.org/Team%3AFJNU-China/Team)
[Collaborations](https://2018.igem.org/Team%3AFJNU-China/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AFJNU-China/Description)
[Design](https://2018.igem.org/Team%3AFJNU-China/Design)
[Experiments](https://2018.igem.org/Team%3AFJNU-China/Experiments)
[Notebook](https://2018.igem.org/Team%3AFJNU-China/Notebook)
[InterLab](https://2018.igem.org/Team%3AFJNU-China/InterLab)
[Model](https://2018.igem.org/Team%3AFJNU-China/Model)
[Results](https://2018.igem.org/Team%3AFJNU-China/Results)
[Demonstrate](https://2018.igem.org/Team%3AFJNU-China/Demonstrate)
[Improve](https://2018.igem.org/Team%3AFJNU-China/Improve)
[Attributions](https://2018.igem.org/Team%3AFJNU-China/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AFJNU-China/Parts)
[Basic Parts](https://2018.igem.org/Team%3AFJNU-China/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AFJNU-China/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AFJNU-China/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AFJNU-China/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AFJNU-China/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AFJNU-China/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AFJNU-China/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AFJNU-China/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AFJNU-China/Hardware)
[Measurement](https://2018.igem.org/Team%3AFJNU-China/Measurement)
[Model](https://2018.igem.org/Team%3AFJNU-China/Model)
[Plant](https://2018.igem.org/Team%3AFJNU-China/Plant)
[Software](https://2018.igem.org/Team%3AFJNU-China/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=FJNU-China)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:FJNU-China/Results

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[HOME](https://2018.igem.org/Team%3AFJNU-China)

TEAM

[Team Members](https://2018.igem.org/Team%3AFJNU-China/Team)
[Collaborations](https://2018.igem.org/Team%3AFJNU-China/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AFJNU-China/Description)
[Design](https://2018.igem.org/Team%3AFJNU-China/Design)
[Experiments](https://2018.igem.org/Team%3AFJNU-China/Experiments)
[Notebook](https://2018.igem.org/Team%3AFJNU-China/Notebook)
[InterLab](https://2018.igem.org/Team%3AFJNU-China/InterLab)
[Model](https://2018.igem.org/Team%3AFJNU-China/Model)
[Results](https://2018.igem.org/Team%3AFJNU-China/Results)
[Demonstrate](https://2018.igem.org/Team%3AFJNU-China/Demonstrate)
[Improve](https://2018.igem.org/Team%3AFJNU-China/Improve)
[Attributions](https://2018.igem.org/Team%3AFJNU-China/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AFJNU-China/Parts)
[Basic Parts](https://2018.igem.org/Team%3AFJNU-China/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AFJNU-China/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AFJNU-China/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AFJNU-China/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AFJNU-China/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AFJNU-China/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AFJNU-China/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AFJNU-China/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AFJNU-China/Hardware)
[Measurement](https://2018.igem.org/Team%3AFJNU-China/Measurement)
[Model](https://2018.igem.org/Team%3AFJNU-China/Model)
[Plant](https://2018.igem.org/Team%3AFJNU-China/Plant)
[Software](https://2018.igem.org/Team%3AFJNU-China/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=FJNU-China)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)