iGEM 2015 — Team Technion_HS_Israel

# Team:Technion HS Israel/Notebook

Technion 2015 HS Team's Wiki

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  Home](https://2015.igem.org/Team%3ATechnion_HS_Israel)

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  Project
  More about our project](https://2015.igem.org/Team%3ATechnion_HS_Israel/Description)

  [Description](https://2015.igem.org/Team%3ATechnion_HS_Israel/Description)
  [Results](https://2015.igem.org/Team%3ATechnion_HS_Israel/Project/Results)

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  Modelling
  We take our math seriously](https://2015.igem.org/Team%3ATechnion_HS_Israel/Modelling)

  [Model Overview](https://2015.igem.org/Team%3ATechnion_HS_Israel/Modelling)
  [Plasmid Loss](https://2015.igem.org/Team%3ATechnion_HS_Israel/PlasmidLoss)
  [Equations](https://2015.igem.org/Team%3ATechnion_HS_Israel/Modelling/Equations)
  [Parameters](https://2015.igem.org/Team%3ATechnion_HS_Israel/Modelling/Parameters)
  [Results](https://2015.igem.org/Team%3ATechnion_HS_Israel/Modelling/Results)
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  Software](https://2015.igem.org/Team%3ATechnion_HS_Israel/Software)

  [Software Overview](https://2015.igem.org/Team%3ATechnion_HS_Israel/Software)
   [Search Engine](https://2015.igem.org/Team%3ATechnion_HS_Israel/Practices/SearchEngine)
  [Constants Database](https://2015.igem.org/Team%3ATechnion_HS_Israel/Constants_Database)
  [Simulation](https://2015.igem.org/Team%3ATechnion_HS_Israel/Software/Simulation)
  [About the Database](https://2015.igem.org/Team%3ATechnion_HS_Israel/LINK_TO_EXPLANATION)
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  Team
  We are a bunch of nerdy nerds](https://2015.igem.org/Team%3ATechnion_HS_Israel/Team)

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  Practices](https://2015.igem.org/Team%3ATechnion_HS_Israel/Practices)

  [Collaborations](https://2015.igem.org/Team%3ATechnion_HS_Israel/Practices/collaborations)
  [Practices](https://2015.igem.org/Team%3ATechnion_HS_Israel/Practices)
  [Search Engine](https://2015.igem.org/Team%3ATechnion_HS_Israel/Practices/SearchEngine)
  [Modeling Tutorials](https://2015.igem.org/Team%3ATechnion_HS_Israel/Practices/modelingTutorials%20)

Contents

* Top
* [Summery](#Summery)
* [Preparation](#Preparation)
* [July](#July)
* [August](#August)
* [September](#September)

# Notebook

Here is a summery of our notebook.

| Week | Design | Cloning | Experiment | Finish new BioBrick |
| --- | --- | --- | --- | --- |
| Jul 5th to Jul 9th (2015) | Primers : OGEM 1 and 2 to open j09855 plasmid .  Gblock :RBS- Aiia with a Gibson overlabs. | we got j09855 part from the distribution kit. | - | - |
| June 12th to July 16th (2015) | Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. | PCR to open the j09855 plasmid.Gibson to add Aiia . | - | BBa\_K1767001 |
| Aug. 2nd to Aug. 6th (2015) | - | We got p0440 part from the distribution kit. We built BBa\_K1767002 and BBa\_K1767004 Constructs by restriction enzymes.  PCR to get the k1767001 plasmid without LuxR . | - | BBa\_K1767002  BBa\_K1767004 |
| Aug. 9th to Aug. 13th (2015) | Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR) | We got I13521 part from the distribution kit. We built BBa\_K1767003 and BBa\_K1767005 Constructs by restriction enzymes. Colony PCR to check if we have LuxR in the plasmid. | - | BBa\_K1767003 BBa\_K1767005 |
| Aug. 16th to Aug. 20th (2015) | - | - | AHL experiment #1 | - |
| Aug. 23rd to Aug. 27th (2015) | Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ). | We got I13003 part from the distribution kit. | AHL experiment #2 | - |
| Aug. 30th to Sept. 9th (2015) | Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA ) | We built BBa\_K1767009 and BBa\_K1767010 Constructs by restriction enzymes.Colony PCR to check if we have YFP in the plasmid. | - | BBa\_K1767009 BBa\_K1767010 |
| Sept. 6th to Sept. 11th (2015) | - | - | AHL experiment # 3 | - |

#### preparation

* 1. LB medium
* 2. Miniprep

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/d/d9/Technion_HS_Israel_pre1_2.jpg)

![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/9/90/Technion_HS_Israel_pre5_2.jpg)

![https://2015.igem.org/File:Technion_HS_Israel_pre6](https://static.igem.org/mediawiki/2015/3/3e/Technion_HS_Israel_pre6_2.jpg)

![https://2015.igem.org/File:Technion_HS_Israel_pre7](https://static.igem.org/mediawiki/2015/9/94/Technion_HS_Israel_pre7_2.jpg)

![https://2015.igem.org/File:Technion_HS_Israel_pre2](https://static.igem.org/mediawiki/2015/8/81/Technion_HS_Israel_pre2_2.jpg)

![https://2015.igem.org/File:Technion_HS_Israel_pre3](https://static.igem.org/mediawiki/2015/e/e6/Technion_HS_Israel_pre3_2.jpg)

![https://2015.igem.org/File:Technion_HS_Israel_pre4](https://static.igem.org/mediawiki/2015/3/31/Technion_HS_Israel_pre4_2.jpg)
### July

[July 12th](#July 12th)
 [July 13th](#July 13th)
 [July 15th](#July 15th)
 [July 20th](#July 20th)
  [July 22th](#July 22th)
 [July 29th](#July 29th)

#### July 12th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/6/64/Technion_HS_Israel_12_7.jpg)
#### July 13th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/5/50/Technion_HS_Israel_13_7.jpg)
#### July 15th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/6/6b/Technion_HS_Israel_15_7_1.jpg)

![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/6/6b/Technion_HS_Israel_15_7_2.jpg)
#### July 20th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/6/6b/Technion_HS_Israel_20_7.jpg)
#### July 22th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/b/bb/Technion_HS_Israel_22_7_1.jpg)

![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/8/86/Technion_HS_Israel_22_7_2.jpg)
#### July 29th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/a/a2/Technion_HS_Israel_29_7.jpg)
### August

[August 2th](#august 2th)
 [August 3th](#august 3th)
 [August 4th](#august 4th)
 [August 5th](#august 5th)
 [August 6th](#august 6th)
 [August 9th](#august 9th)
 [August 10th](#august 10th)
 [August 11th](#august 11th)
 [August 12th](#august 12th)
 [August 13th](#august 13th)
 [August 16th](#august 16th)
 [August 17th](#august 17th)
 [August 18th](#august 18th)
 [August 19th](#august 19th)
 [August 20th](#august 20th)
 [August 23th](#august 23th)
 [August 24th](#august 24th)
 [August 25th](#august 25th)
 [August 26th](#august 26th)
 [August 27th](#august 27th)
 [August 29th](#august 29th)
 [August 30th](#august 30th)
 [August 31th](#august 31th)

#### august 2th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/f/f6/Technion_HS_Israel_2_8.jpg)
#### august 3th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/8/85/Technion_HS_Israel_3_8_1.jpg)

![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/a/a7/Technion_HS_Israel_3_8_2.jpg)
#### august 4th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/2/24/Technion_HS_Israel_4_8_1.jpg)

![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/c/ca/Technion_HS_Israel_4_8_2.jpg)
#### august 5th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/b/b8/Technion_HS_Israel_5_8.jpg)
#### august 6th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/0/02/Technion_HS_Israel_6_8_1.jpg)

![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/1/12/Technion_HS_Israel_6_8_2.jpg)
#### august 9th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/3/36/Technion_HS_Israel_9_8.jpg)
#### august 10th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/8/8e/Technion_HS_Israel_10_8_1.jpg)

![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/7/73/Technion_HS_Israel_10_8_2.jpg)
#### august 11th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/c/ce/Technion_HS_Israel_11_8.jpg)
#### august 12th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/2/22/Technion_HS_Israel_12_8.jpg)
#### august 13th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/f/f9/Technion_HS_Israel_13_8.jpg)
#### august 16th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/d/d7/Technion_HS_Israel_16_8.jpg)
#### august 17th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/2/25/Technion_HS_Israel_17_8.jpg)
#### august 18th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/e/e7/Technion_HS_Israel_18_8.jpg)
#### august 19th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/d/d0/Technion_HS_Israel_19_8.jpg)
#### august 20th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/d/d7/Technion_HS_Israel_20_8.jpg)
#### august 23th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/1/12/Technion_HS_Israel_23_8.jpg)
#### august 24th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/e/ee/Technion_HS_Israel_24_8.jpg)
#### august 25th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/5/52/Technion_HS_Israel_25_8.jpg)
#### august 26th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/f/fe/Technion_HS_Israel_26_8.jpg)
#### august 27th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/5/56/Technion_HS_Israel_27_8.jpg)
#### august 29th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/d/d7/Technion_HS_Israel_29_8.jpg)
#### august 30th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/b/bb/Technion_HS_Israel_30_8.jpg)
#### august 31th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/1/11/Technion_HS_Israel_31_8.jpg)
### September

[September 1th](#september 1th)
 [September 2th](#september 2th)
 [September 3th](#september 3th)
 [September 5th](#september 5th)
 [September 6th](#september 6th)
 [September 7th](#september 7th)

#### september 1th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/e/e6/Technion_HS_Israel_1_9.jpg)
#### september 2th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/c/c7/Technion_HS_Israel_2_9.jpg)
#### september 3th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/8/84/Technion_HS_Israel_3_9.jpg)
#### september 5th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/7/71/Technion_HS_Israel_5_9.jpg)
#### september 6th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/8/8a/Technion_HS_Israel_6_9_1.jpg)

![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/5/5b/Technion_HS_Israel_6_9_2.jpg)
#### september 7th

![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/2/23/Technion_HS_Israel_7_9.jpg)