iGEM 2017 — Team SHSBNU_China

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:SHSBNU China/Notebook

SHSBNU-China iGEM 2017 Home Page

![](https://static.igem.org/mediawiki/2017/e/ee/SHSBNU_17_background.jpg)

[![](https://static.igem.org/mediawiki/2017/a/aa/SHSBNU_17_Banner.jpg)](https://2017.igem.org/Team%3ASHSBNU_China)

[Project](https://2017.igem.org/Team%3ASHSBNU_China/Project)
|
[Model](https://2017.igem.org/Team%3ASHSBNU_China/Model)
|
[HP](https://2017.igem.org/Team%3ASHSBNU_China/HP)
|
[Safety](https://2017.igem.org/Team%3ASHSBNU_China/Safety)
|
[Parts](https://2017.igem.org/Team%3ASHSBNU_China/Parts)
|
[Team](https://2017.igem.org/Team%3ASHSBNU_China/Team)
|
[Achievement](https://2017.igem.org/Team%3ASHSBNU_China/Achievement)

Achievement

[Achievement](https://2017.igem.org/Team%3ASHSBNU_China/Achievement)
|
[Notebook](https://2017.igem.org/Team%3ASHSBNU_China/Notebook)

Notebook

SHSBNU\_China iGEM2017 would like to share with you the document of the work done every week for our project. Although we have only 6 team members in the team, we go all out to do the experiments.

Week1

**10/07/2017-16/07/2017**

Do Interlab experiment at Peking iGEM team lab.

Week2

**17/07/2017-23/07/2017**

Transform chromoproteins BBa\_K1033910, BBa\_K1033916, BBa\_K592010, into a pSEVA321 backbone(Provided by Bluepha).

Order Sequence synthesis of ThsS,ThsR,TtrS,TtrR by synbio, Suzhou.

Week3

**24/07/2017-30/07/2017**

Transform chromoproteins BBa\_K1033910, BBa\_K1033916, BBa\_K592010, into a pSEVA321 backbone(Provided by Bluepha).

Week4

**31/07/2017-06/08/2017**

Order Primers for constructing BBa\_K2507004, BBa\_K2507006, BBa\_K2507008& BBa\_K2507013 parts by Golden Gate.

Week5

**07/08/2017-13/08/2017**

Receive synthesized sequences from synbio, and started PCR and then Golden Gate.

Failed several times of constructing the parts.

Week6

**14/08/2017-20/08/2017**

Succeed construct BBa\_K2507004, BBa\_K2507006, BBa\_K2507008& BBa\_K2507013.

Order primers for constructing BBa\_K2507009, BBa\_K2507010, BBa\_K2507011, BBa\_K2507012& BBa\_K25070014, BBa\_K2507015, BBa\_K2507016, BBa\_K2507019.

Get E.coli Nissle 1917 from BNDS\_China.

Week7

**21/08/2017-27/08/2017**

Construct BBa\_K2507009, BBa\_K2507010, BBa\_K2507011, BBa\_K2507012& BBa\_K25070014, BBa\_K2507015, BBa\_K2507016, BBa\_K2507019.

Test which chromoprotein can be obvious in curry (replace excrement) for the first time.

Week8

**28/08/2017-03/09/2017**

Qualitative test the response curve of ThsSR-gfp/ TtrSR-gfp.

Qualitative test the response curve of ThsSR-pink, purple, blue/ TtrSR-pink, purple, blue.

Week9

**04/09/2017-10/09/2017**

Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the first time.

Qualitative test the response curve of ThsSR-gfp/ TtrSR-gfp in aerobic and anaerobic condition.

Go to the clinical laboratory of navy general hospital and made an interview

Week10

**11/09/2017-17/09/2017**

Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the second time.

Construct Biobrick BBa\_K2507005, BBa\_K2507007.

Construct Biobrick BBa\_K2507012, BBa\_K25070017.

Week11

**18/09/2017-24/09/2017**

Transform Biobrick BBa\_K2507004 with BBa\_K2507012, and BBa\_K2507006 with BBa\_K2507017 into E. Coli Top10.

Test ThsSR-vio/TtrSR-vio system.

Week12

**25/09/2017-01/10/2017**

Construct Biobrick BBa\_K2507000-004.

Establish synthetic biology club in SHSBNU.

Update safety form.

Week13

**02/10/2017-08/10/2017**

Start test chemical transform of *Bacillus subtilis.*

Design Safety devices.

Week14

**09/10/2017-15/10/2017**

Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the third time.

Model our project.

Week15

**16/10/2017-22/10/2017**

Send part to Genscript.

[![](https://static.igem.org/mediawiki/2017/4/44/SHSBNU_Logo_256X256.jpg)](http://www.shsbnu.net)
[![](https://static.igem.org/mediawiki/2017/1/1f/Azir_Logo_256X256.jpg)](http://www.azirquantum.net.cn)
[![](https://static.igem.org/mediawiki/2017/6/60/IGEM_Logo_256X256.jpg)](https://2017.igem.org)
[![](https://static.igem.org/mediawiki/2017/b/bc/Bluepha_Logo_256X256.jpg)](http://www.bluepha.com)

iGEM 2017

Made by Azir.

The Second High School Attached Beijing Normal University International Division

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)


[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:SHSBNU China/Experiments

[![](http://placehold.it/350x150)](https://2017.igem.org/Team%3ASHSBNU_China)

MENU

[HOME](https://2017.igem.org/Team%3ASHSBNU_China)

TEAM

[Team](https://2017.igem.org/Team%3ASHSBNU_China/Team)
[Collaborations](https://2017.igem.org/Team%3ASHSBNU_China/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3ASHSBNU_China/Description)
[Design](https://2017.igem.org/Team%3ASHSBNU_China/Design)
[Experiments](https://2017.igem.org/Team%3ASHSBNU_China/Experiments)
[Notebook](https://2017.igem.org/Team%3ASHSBNU_China/Notebook)
[InterLab](https://2017.igem.org/Team%3ASHSBNU_China/InterLab)
[Contribution](https://2017.igem.org/Team%3ASHSBNU_China/Contribution)
[Model](https://2017.igem.org/Team%3ASHSBNU_China/Model)
[Results](https://2017.igem.org/Team%3ASHSBNU_China/Results)
[Demonstrate](https://2017.igem.org/Team%3ASHSBNU_China/Demonstrate)
[Improve](https://2017.igem.org/Team%3ASHSBNU_China/Improve)
[Attributions](https://2017.igem.org/Team%3ASHSBNU_China/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3ASHSBNU_China/Parts)
[Basic Parts](https://2017.igem.org/Team%3ASHSBNU_China/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3ASHSBNU_China/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3ASHSBNU_China/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3ASHSBNU_China/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3ASHSBNU_China/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3ASHSBNU_China/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3ASHSBNU_China/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3ASHSBNU_China/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3ASHSBNU_China/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3ASHSBNU_China/Hardware)
[Measurement](https://2017.igem.org/Team%3ASHSBNU_China/Measurement)
[Model](https://2017.igem.org/Team%3ASHSBNU_China/Model)
[Plant](https://2017.igem.org/Team%3ASHSBNU_China/Plant)
[Software](https://2017.igem.org/Team%3ASHSBNU_China/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=SHSBNU_China)

# SHSBNU\_China

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

##### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

##### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:SHSBNU China/Results

[![](http://placehold.it/350x150)](https://2017.igem.org/Team%3ASHSBNU_China)

MENU

[HOME](https://2017.igem.org/Team%3ASHSBNU_China)

TEAM

[Team](https://2017.igem.org/Team%3ASHSBNU_China/Team)
[Collaborations](https://2017.igem.org/Team%3ASHSBNU_China/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3ASHSBNU_China/Description)
[Design](https://2017.igem.org/Team%3ASHSBNU_China/Design)
[Experiments](https://2017.igem.org/Team%3ASHSBNU_China/Experiments)
[Notebook](https://2017.igem.org/Team%3ASHSBNU_China/Notebook)
[InterLab](https://2017.igem.org/Team%3ASHSBNU_China/InterLab)
[Contribution](https://2017.igem.org/Team%3ASHSBNU_China/Contribution)
[Model](https://2017.igem.org/Team%3ASHSBNU_China/Model)
[Results](https://2017.igem.org/Team%3ASHSBNU_China/Results)
[Demonstrate](https://2017.igem.org/Team%3ASHSBNU_China/Demonstrate)
[Improve](https://2017.igem.org/Team%3ASHSBNU_China/Improve)
[Attributions](https://2017.igem.org/Team%3ASHSBNU_China/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3ASHSBNU_China/Parts)
[Basic Parts](https://2017.igem.org/Team%3ASHSBNU_China/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3ASHSBNU_China/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3ASHSBNU_China/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3ASHSBNU_China/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3ASHSBNU_China/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3ASHSBNU_China/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3ASHSBNU_China/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3ASHSBNU_China/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3ASHSBNU_China/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3ASHSBNU_China/Hardware)
[Measurement](https://2017.igem.org/Team%3ASHSBNU_China/Measurement)
[Model](https://2017.igem.org/Team%3ASHSBNU_China/Model)
[Plant](https://2017.igem.org/Team%3ASHSBNU_China/Plant)
[Software](https://2017.igem.org/Team%3ASHSBNU_China/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=SHSBNU_China)

# SHSBNU\_China

# Results

Here you can describe the results of your project and your future plans.

##### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

##### You should also describe what your results mean:

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

##### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

##### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)