iGEM 2016 — Team KoreaSonyeodul

[![](https://static.igem.org/mediawiki/2016/8/8b/HQ_page_logo.jpg)](https://2016.igem.org)

# Team:KoreaSonyeodul/Notebook

* [![](https://static.igem.org/mediawiki/2016/a/ab/Home-icon-silhouette.png)](https://2016.igem.org/Team%3AKoreaSonyeodul)
* [![](https://static.igem.org/mediawiki/2016/c/c0/Networking.png)](https://2016.igem.org/Team%3AKoreaSonyeodul/Team)
  + [COLLABORATIONS](https://2016.igem.org/Team%3AKoreaSonyeodul/Collaborations)
* ![](https://static.igem.org/mediawiki/2016/5/5a/Businessman-in-apresentation-with-a-graphic-on-a-board_%281%29.png)
  + [COLLABORATIONS](https://2016.igem.org/Team%3AKoreaSonyeodul/Description)
  + [DESIGN](https://2016.igem.org/Team%3AKoreaSonyeodul/Design)
  + [EXPERIMENTS](https://2016.igem.org/Team%3AKoreaSonyeodul/Experiments)
  + [PROOF](https://2016.igem.org/Team%3AKoreaSonyeodul/Proof)
  + [DEMONSTRATE](https://2016.igem.org/Team%3AKoreaSonyeodul/Demonstrate)
  + [RESULTS](https://2016.igem.org/Team%3AKoreaSonyeodul/Results)
  + [NOTEBOOK](https://2016.igem.org/Team%3AKoreaSonyeodul/Notebook)
* [![](https://static.igem.org/mediawiki/2016/0/0f/Molecule.png)](https://2016.igem.org/Team%3AKoreaSonyeodul/Parts)
  + [BASIC](https://2016.igem.org/Team%3AKoreaSonyeodul/Basic_Part)
* [![](https://static.igem.org/mediawiki/2016/0/0b/Safe-shield-protection.png)](https://2016.igem.org/Team%3AKoreaSonyeodul/Safety)
* [![](https://static.igem.org/mediawiki/2016/e/e0/Attribution.png)](https://2016.igem.org/Team%3AKoreaSonyeodul/Attributions)
* ![](https://static.igem.org/mediawiki/2016/6/69/Two-stick-man-variants-shaking-hands.png)
  + [PUBLIC OUTREACH](https://2016.igem.org/Team%3AKoreaSonyeodul/Public_Outreach)
  + [ETHICS](https://2016.igem.org/Team%3AKoreaSonyeodul/Ethics)
  + [COLLABORATION](https://2016.igem.org/Team%3AKoreaSonyeodul/Collaboration)
  + [INTEGRATED PRACTICES](https://2016.igem.org/Team%3AKoreaSonyeodul/Integrated_Practices)
* ![](https://static.igem.org/mediawiki/2016/b/b7/Medal.png)
  + [Gold Medal](https://2016.igem.org/Team%3AKoreaSonyeodul/HP/Gold)
  + [Silver Medal](https://2016.igem.org/Team%3AKoreaSonyeodul/HP/Silver)
* [![](https://static.igem.org/mediawiki/2016/3/3e/Earth-globe.png)](https://2016.igem.org/Team%3AKoreaSonyeodul/Engagement)
* [![](https://static.igem.org/mediawiki/2016/5/54/Promoting.png)](https://2016.igem.org/Team%3AKoreaSonyeodul/Entrepreneurship)
* [![](https://static.igem.org/mediawiki/2016/4/44/Creative-commons.png)](https://2016.igem.org/Team%3AKoreaSonyeodul/Credits)

# NOTE

## Report Journal

| Day | Description | Contents | Participants |
| --- | --- | --- | --- |
| March 11th | Brainstorming | Contents | All Members |
| March 18th | Inquiry of potsntial supervisors/campaign report | Contents | All Members |
| March 23rd | Competition Application | Decide on the subject of the project: Mealworm’s degradation of plastic | All Members |
| March 25th | Planning | Specifically plan out Experimental Methods and Procedures | All Members |
| March 29th | Order Mealworms | Order Mealworms | Seunghee Han |
| March 31st | 1st Experiment | 4g of Styrofoam, three sub-experiments each have a control and experimental containers with 20 mealworms each:   Cool vs. Warm   Light vs. No Light   With other food vs. Just plastic | All Members |
| April 7th | 2nd Experiment | Check on the progress of the three experiment/document results | Soomin Lee Yemi Shin Jimin Sun Heesun Sim |
| April 14th | 3rd Experiment | Check on the progress of the three experiment/document results | Jungeun Huh Seunghee Han S. Kang |
| April 21st | 4th Experiment | Check on the progress of the three experiment/document results | Soomin Lee Yemi Shin Jimin Sun Heesun Sim |
| May 15th | Twitter Account/Contact Professor | Created Twitter/Contacted Professor for advice | Yemi Shin |
| June 1st | Team Meeting | Revise Plans, and further specify plans, but couldn’t work with DNA synthesis because the kit had not arrived. | All Members |
| June 13th | Mini-Bio Forum | Each one of us presented different sections of the Bio-Tech chapter. | All Members |
| July 13th | Arrival of the DNA kit | DNA kit finally, finally arrived | Soomin Lee |
| July 8th | 1st Visit to Professor | Got advice for our project in the future, talked about ways in which the lab could support our project | All Members |
| July 8th | Collaboration with Korea University Team | Presentation of both team’s subjects and time for giving advice | All Members |
| July 11th | Meeting with Professor Cho | Before this official meeting, we only had communications throughout e-mails with our instructor, who is a synthetic biology professor in Korea University. In this meeting in the Korea University, we specified the experiments that we will conduct. Then, we planned the specific dates of experiment | All Members |
| August 12th | Planning the experiment | Seunghee Han and Yemi Shin visited the Korea University to discuss about how we are going to further the experiment. At first our team put the main focus on enhancing the speed of degradation. However, after talking with professor Choi, we’ve decided to recombine the mealworm’s gut dna, DH5+alpha and PUC 19. We also scheduled our experiments | Seunghee Han Yemi Shin |
| August 16th | Chemical Transformation | We transfomed E-coli, DH5-alpha using PUC19 in Korea University | Seunghee Han Soomin Lee |
| August 26th | Mealworm Related Measurements | After feeding 40 mealworms with only plastic for 5 weeks, we measured the length and the weight of the 20 samples. The average weight is 0.177g per worm and the original length is about 3+- 0.4cm. Compared to the original length 2+-0.2cm, it is noticeable that mealworms are possible to continue their lives with plastics as their only source of nutrient | S. Kang Jungeun Huh Jimin Sun |
| September 9th |  | Visited the Korea University to practice E-coli cell streaking. Did the three sector T streak. Organisms are streaked through the first sector using either a disposable loop or needle, or a reusable loop, usually with nichrome or platinum wire, and then passed through a Bunsen burner for sterilization |  | Seunghee Han Yemi Shin |
| September 22nd | Chemical Transformation of E.coli | Visited Korea University and experimented the chemical transformation of the e.coli strain DH5 alpha and pBBRMCS2-Pcon-yfaL-PETase | Seunghee han Jeongeun huh |
| October 7th | Checking and Marking off the Judging form | Together with our TA, we completed our Judging Forms and organized all the final materials needed for the Giant Jamboree | Soomin Lee Seohyun Kang |
| October 10th | Semi-final check | Again with the TA we submitted all the parts to the registry (PETase) and double checked the wiki | Yemi Shin JImin Sun |
| October 16th | Final check | Final check of the wiki, made revisions | Yemi Shin Heesun Sim |

[![](https://static.igem.org/mediawiki/2016/b/bf/HQ_menu_logo.jpg)](https://2016.igem.org)