iGEM 2022 — Team hizju-china

{% extends "layout.html" %}
{% block title %}Notebook{% endblock %}
{% block lead %}Document the dates you worked on your project. This should be a detailed account of the work done each
day for your project.{% endblock %}
{% block page\_content %}
![](https://static.igem.wiki/teams/4177/wiki/wiki/note/notebook.png)

![](https://static.igem.wiki/teams/4177/wiki/wiki/top.png)

![](https://static.igem.wiki/teams/4177/wiki/wiki/note/experimentnotebook.png)

April,1
Experiment basic operation exercise, promoter PCR attempt.

May,1
gRNA construction, promoter PCR attempt.

Jane,1
harmOR10 gene integration attempt, promoter FUS1 PCR.

July,9
Related experiments of reporter plasmid construction;
Related experiments of harmOR10 gene integration（Culture BY4741 yeast seed solution）.

July, 10
Related experiments of reporter plasmid construction (The promoter, plasmid skeleton and mCherry fragment were
digested respectively, and the gel was cut and recovered);
Related experiments of harmOR10 gene integration（The seed liquid was transferred to a shake flask and the
sensitive state was prepared after 5h）.

July,13
Related experiments of reporter plasmid construction (the scribe plate grows more colonies, and verify the primer
synthesis);
Related experiments of harmOR10 gene integration（Competent preparation was completed and BY4741 seed solution was
inoculated）;
Experiment of Schizonepeta alcohol fermentation（activation of strain bank strains (3-1 / 3-2 / 3-3 / 3-4)）.

July,14
Related experiments of reporter plasmid construction (redesigned the primers and performed PCR again, observe the
results);
Related experiments of harmOR10 gene integration（Continue to raise yeast (grow into single colonies of appropriate
size in solid medium for 3 days);
Experiment of Schizonepeta alcohol fermentation (Transfer to shake flask; Preserve the bacteria and inoculate into
the peptonea tube (seed solution culture)).

July,15
Related experiments of reporter plasmid construction (FUS1 and mCherry plasmids were tested, greemix pcr50 degree
was 1.5min, and there was almost no band after gel running, It may be necessary to perform the ligation
transformation of FUS1 and mCherry again, or re inoculate the plate.);
Experiment of Schizonepeta alcohol fermentation (Transfer to galactose medium).

July,16
Related experiments of reporter plasmid construction (PCR was performed on mcerry and FUS1 that had not been
enzymatically digested. There were bands in the gel. PCR was recovered, and the DNA concentration was measured. It
was placed in the refrigerator at - 20 degrees; FUS1 amplification failed and was discarded; Digested mCherry and
FUS1 with 10 μ LT4 ligase enzyme ligation system enzyme ligation, inoculate amp plate and put it in 37 °
incubator; PCR 4 tubes of FUS1);
Experiment of Schizonepeta alcohol fermentation (Transfer to galactose medium and ferment for two days).

July,17
Related experiments of reporter plasmid construction (Enzymatic ligation, transformation and inoculation).
Related experiments of harmOR10 gene integration（gRNA plasmid skeleton extraction, gRNA annealing, GRNs plasmid
skeleton digestion）.

July,18
Related experiments of reporter plasmid construction (Checkpoint, FUS1 and mCherry enzyme linked, transformed,
coated and inoculated);
Related experiments of harmOR10 gene integration（Enzyme linked, transformation）;
Experiment of Schizonepeta alcohol fermentation (GC, Sampling, extraction, sample preparation and storage in
refrigerator).

July,19
Related experiments of harmOR10 gene integration（Enzyme linked, grna2 and grna3 fragments were enzymatically
linked to the plasmid backbone and transformed into E. coli）

July,20
Related experiments of reporter plasmid construction (Two primers were used to check the spots respectively, and
both failed);
Related experiments of harmOR10 gene integration（Plasmids were extracted, sequenced, and bacteria were preserved.
gRNA2, 3 test points, and the test points were successful; Amplification of gRNA2, 3）;
Experiment of Schizonepeta alcohol fermentation (Transfer into 30ml shake flask (glucose medium, culture seed
liquid))

July,21
Related experiments of reporter plasmid construction (Plasmid extraction, bacteria preservation, sequencing; Check
point: the two primers are mixed and the check point is successful; Select points and expand cultivation.);
Related experiments of harmOR10 gene integration（transformation after sequencing）;
Experiment of Schizonepeta alcohol fermentation (Transfer to galactose medium, transfer bacteria 1 and 2 to six
shake flasks (glucose medium)).

July,22
Related experiments of reporter plasmid construction (Sequencing, Plasmid Extraction, concentration determination
and recording);
Related experiments of harmOR10 gene integration（Construct yeast competent state and put it in 4 ℃ refrigerator）;

Experiment of Schizonepeta alcohol fermentation (The two groups of bacteria were centrifuged together with the
culture medium, and then the precipitates were washed twice with sterile water, transferred to galactose culture
medium, and cultured in a shaking table at 30 ℃).

July,24
Related experiments of harmOR10 gene integration（Transformation (grna2, 3 and whether to add donor, four groups in
total)）;
Experiment of Schizonepeta alcohol fermentation (Sampling: take out the triangular flask from the shaker, add 1ml
of bacterial solution into 2ml centrifuge tube, add 1ml of ethyl acetate into each tube, mix it with a vortex for
30s, shake it on the shaker for 40min, mix it again, shake it for 40min, and take the upper layer.).

July,25
Experiment of Schizonepeta alcohol fermentation (Prepare YPD (A));
Transformation of Schizonepeta alcohol fermentation strain (Inoculate gRNA plasmid bacteria and plasmid bacteria
containing the gene to be introduced).

July,26
Experiment of Schizonepeta alcohol fermentation (3 and 4 were inoculated into test tubes and cultured in a
shaker);
Transformation of Schizonepeta alcohol fermentation strain (Plasmid extraction, gRNA extraction, put in - 20
degree refrigerator.).

July,27
Related experiments of harmOR10 gene integration（Pick points, break cells, PCR, glue run, fail）;
Experiment of Schizonepeta alcohol fermentation (Bacteria 3 and 4 were inoculated into six culture media and
cultured in a shaker at 30 ℃ for 24h);
Transformation of Schizonepeta alcohol fermentation strain (Pick points, break cells, PCR, glue run, fail.).

July,28
Related experiments of harmOR10 gene integration（Cell breakage, PCR checkpoint, PCR recovery. The result is not up
to standard and is discarded.）;
Experiment of Schizonepeta alcohol fermentation (Transfer to galactose medium and culture for 24h).

July,30
Experiment of Schizonepeta alcohol fermentation (extraction).

August,1
Related experiments of reporter plasmid construction (Promoter PCR);
Related experiments of harmOR10 gene integration（Checkpoint (phantase)）;
Experiment of Schizonepeta alcohol fermentation (GC-MS chromatography).

August,2
Related experiments of reporter plasmid construction (pcr fus1);
Related experiments of harmOR10 gene integration（pcr donor，）;

August,3
Related experiments of reporter plasmid construction (Yeast plasmid with mCherry was extracted, digested, purified
and recovered, linked with FUS1 two fragments, transformed in large intestine and inoculated);
Related experiments of harmOR10 gene integration（Construction, transformation and inoculation of competent yeast）;

Experiment of Schizonepeta alcohol fermentation (Seed liquid, inoculation);
Transformation of Schizonepeta alcohol fermentation strain (Construction, transformation and inoculation of
competent yeast).

August,4
Related experiments of reporter plasmid construction (Test point, inoculation at point 8, culture for 12-16h);

Experiment of Schizonepeta alcohol fermentation (Transit glucose medium).

August,5
Related experiments of reporter plasmid construction (Bacteria preservation, sequencing and plasmid extraction.);

Experiment of Schizonepeta alcohol fermentation (Transfer from glucose medium to galactose medium and incubate for
48 hours);

August,6
Related experiments of harmOR10 gene integration（At the test point, the yeast is broken (boiling solution, 20ul,
98 ℃, 30min). After boiling, the yeast is centrifuged, and the supernatant is taken for PCR with phantase.）;
Transformation of Schizonepeta alcohol fermentation strain (Jl1491 plasmid was extracted and PCR (primers: cyqr,
ADHF)).

August,7
Experiment of Schizonepeta alcohol fermentation (Extraction, GC);
Transformation of Schizonepeta alcohol fermentation strain (Take the plasmid of jl1491 gRNA, transform it into
yeast am3-2, and coat the plate).

August,8
Related experiments of harmOR10 gene integration（Plasmid extraction, PCR, transformation, plating）;
Transformation of Schizonepeta alcohol fermentation strain (PCR JL1491, transformation, plating).

August,11
Related experiments of harmOR10 gene integration（The phenomenon of paste plate (too dense colonies) appeared in
transformation, because the cas9 gene contained in the wild-type BY4741 strain used does not match the gRNA）;
Experiment of Schizonepeta alcohol fermentation (Spectrum observation: in the last batch of fermentation
experiments, all strains except 3-1 had product peaks, of which 3-2 and 3-4 products were relatively more);
Transformation of Schizonepeta alcohol fermentation strain (Pick and check points).

August,13
Related experiments of harmOR10 gene integration（Plasmid extraction, PCR, transformation）;
Transformation of Schizonepeta alcohol fermentation strain (Plasmid extraction, PCR, unsuccessful, re
inoculation).

August,14
Transformation of Schizonepeta alcohol fermentation strain (Plasmid extraction, PCR, transformation, culture).

August,16
Related experiments of harmOR10 gene integration（Two colonies grow out of the ste plate - all the test points have
correct bands, and the PCR products are sent for testing）;

August,17
Integration of reporter plasmid and harmOR10（Report plasmid PCR, gel run test, recovery, concentration
measurement, preservation; Ste-harmor10 1 / 2 each preserved bacteria, shake the bottle for 5h, prepare the
sensitive state, store it at 4 ℃, and collect the sequencing results of harmor10 gene. The results are correct
compared with the original genome）；

August,18
Integration of reporter plasmid and harmOR10（P426 plasmid was extracted; Ste-harmor10 transformation）；

August,19
Integration of reporter plasmid and harmOR10（The p426-21 plasmid was extracted, the concentration was measured,
and stored）；
Transformation of Schizonepeta alcohol fermentation strain (Prepare 3-2 / 3-4 competent state; Jl1491
vaccination).

August,20
Transformation of Schizonepeta alcohol fermentation strain (Jl1491 inoculation is still not long; Use JL1491 on
the amp-lb plate coated a few days ago, pick a spot, PCR, failed).

August,21
Integration of reporter plasmid and harmOR10（Both 2-2 and 1-2 grow a colony, pick a spot to verify (phanta PCR),
run the glue, and the band is about 1500 ∓500）；
Transformation of Schizonepeta alcohol fermentation strain (Plasmid PCR, gel run verification).

August,23
Transformation of Schizonepeta alcohol fermentation strain (PCR with KOD one enzyme).

August,26
Integration of reporter plasmid and harmOR10（Re check the spot and take photos, but it does not run out of the
strip）；
Transformation of Schizonepeta alcohol fermentation strain (Reconvert with 3-2 competent state and inoculate).

Benzaldehyde fluorescence concentration detection (Ste-harmOR10-2 strain was amplified and cultured in
benzaldehyde with concentration gradient for 1 day).

August,27
Benzaldehyde fluorescence concentration detection (Fluorescence concentration detection; No regular results were
found).

August,28
Experiment of Schizonepeta alcohol fermentation (3-2 redo competent state, transformation).

August,29
Transformation of Schizonepeta alcohol fermentation strain (there are two points on the 8.23 board, and there are
no results; The plasmid of p426 was re extracted).
Benzaldehyde fluorescence concentration detection (Medium with gradient benzaldehyde concentration was transferred
and cultured for one day).

August,30
Transformation of Schizonepeta alcohol fermentation strain (There were many colonies in both plates).
Benzaldehyde fluorescence concentration detection (Fluorescence concentration and biomass were detected, but no
regular results were presented).

August,31
Transformation of Schizonepeta alcohol fermentation strain (Test points (8 for each plate, boiling,
centrifugation, phanta PCR, glue running) showed that there were bands around 2000, which were 3, 8 for 1 and 1, 2
and 4 for 2; Select 1-8 and 2-2 for sequencing and then connect with seed liquid for amplification for one day).

September,1
Experiment of Schizonepeta alcohol fermentation (Mlpla transfer strain fermentation experiment: transfer the seed
liquid into glucose YPD (a) shake flask).
Transformation of Schizonepeta alcohol fermentation strain (Mlpla gene integration sequencing was successful, and
the seed liquid was ready to integrate the neps1 gene, and then the Neps1 plasmid bacteria)

September,2
Experiment of Schizonepeta alcohol fermentation (Transfer to galactose medium).
Transformation of Schizonepeta alcohol fermentation strain (Neps1 donor PCR and integration).

September,3
Experiment of Schizonepeta alcohol fermentation (Sampling (24h)).

September,4
Experiment of Schizonepeta alcohol fermentation (Sampling (48h)).

September,5
Experiment of Schizonepeta alcohol fermentation (Seed liquid was inoculated (strains 1 and 2 after neps1
integration, and strain 1 without neps1 integration was used as the control group)).
Transformation of Schizonepeta alcohol fermentation strain (To verify whether neps1 insertion was successful, two
colonies were selected for sequencing).
Plasmid loss of Schizonepeta lactone strain (Inoculated strain).
Discussion with our PI (The self-contained promoter of ste2 is not strong enough, and the donor integrates the gal
promoter into the site; Positive control: keep ste2 gene, only do reporter gene integration, and test whether FUS1
promoter is effective; Fermentation experiment: compare the integrated neps1 strain and the non integrated neps1
strain, and observe the catalytic efficiency of neps1. If the catalytic efficiency is low, consider making
multiple copies).

September,6
Harmor10 gene integration (New) (Inoculate harmor10 plasmid bacteria and by4741-cas9 yeast).
Experiment of Schizonepeta alcohol fermentation (Seed liquid transfer).
Plasmid loss of Schizonepeta lactone strain (Inoculate the bacterial solution of Schizonepeta lactone No. 1 and
No. 2 cultured for 24 hours into the new test tube at the ratio of 1%).
Preparation of positive control bacteria (Inoculation of reporter plasmid and BY4741-cas9).

September,7
Experiment of Schizonepeta alcohol fermentation (After washing, the strain was connected to YPD medium and
cultured in a 30 ° shaker for 24h).
Plasmid loss of Schizonepeta lactone strain (Inoculate the bacterial solution of Schizonepeta lactone No. 1 and
No. 2 cultured for 24 hours into the new test tube at the ratio of 1%).

September,8
Experiment of Schizonepeta alcohol fermentation (Sampling and preparation of fermentation broth and storage in -
20 ℃ refrigerator).
Plasmid loss of Schizonepeta lactone strain (Strain 1 and strain 2 were connected to the plate by scribing and
placed in a 30 degree incubator).

September,10
Losing plasmid of schizonepeta tenuifolia lactone strain (Pick up the single colony (6 in total) on the plate and
mix it with sterile water, and apply it to YPD-G418 plate, SE-URA-G418 plate and SE-HIS-G418 plate respectively.)

Preparation of positive control bacteria (Positive control bacteria sampling verification, fail.)

September,11
Preparation of HarmOR10-GAL strain (HarmOR10-GAL plate spot selection verification, sending for testing (No. 5 and
No. 7 bacteria), and seed liquid culture.)

September,12
Transformation of HarmOR10-GAL strain (Competent preparation, fus1-mCherry gene integration.)
Preparation of positive control bacteria (Competent preparation, fus1-mCherry gene fragment integration.)

September,15
Fermentation experiment (Seed liquid inoculation (NEPS1 introduced strain))

September,16
Fermentation experiment (Seed liquid transfer)

September,17
Fermentation experiment (Transfer to galactose medium)

September,18
Fermentation experiment (Sampling, sample preparation, GCMS detection)

September,22
Fermentation experiment (Inoculation of seed liquid (starting strain, MLPLA modified strain))

September,23
Fermentation experiment (Seed liquid transfer)

September,24
Fermentation experiment (Transfer to galactose medium)

September,25
Fermentation experiment (Sampling, sample preparation, GCMS detection)

{% endblock %}

{% extends "layout.html" %}
{% block title %}Engineering Success{% endblock %}
{% block lead %}Demonstrate engineering success in a part of your project by going through at least one iteration of the
engineering design cycle.{% endblock %}
{% block page\_content %}
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/engineeringsuccess.png)

![](https://static.igem.wiki/teams/4177/wiki/wiki/top.png)

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/detectionsystem.png)

Gene acquisition and plasmid construction

We used HarmOR10 gene which comes from helicoverpa armigera as a receptor for benzaldehyde detection. To test the
receptor, we used FUS1 promoter from the yeast GPCR signaling pathway to link with the mCherry gene, forming a
reporter gene fragment.

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig1.png)

Due to the low GC ratio of the FUS1 promoter fragment, our PCR experiment failed many times. We redesigned the
fragment by inserting three base pairs from the Fus1 gene at the start of the mCherry fragment, finally achieved
successful amplification.

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig2.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig3.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig4.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig5.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig61.png)

*Gene acquisition* and plasmid construction

To ensure the expression efficiency of HarmOR10, we used CRISPR-Cas9 system to knock out the yeast's own GPCR
receptor Ste2 in the original strain BY4741-Cas9, and replaced the HarmOR10 gene at the position of the Ste2 gene.

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig7.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig8.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig9.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig10.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig11.png)

Based on the strain named harm-1 in which we successfully integrate harmOR10 gene, we integrate Fus1p-mCherry gene
on the verified yeast gene integration site int21 to construct harmOR10 receptor test strain T1.

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig12.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig13.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig14.png)

We integrated fus1p-mCherry gene into the original strain BY4741-Cas9 to form a positive control strain.

Test of the strain

The fluorescence intensity and biomass of T1 strain was tested after cultivated in full nutrient SCD medium with a
benzaldehyde concentration gradient for 24 hours

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/table.png)

Optimization

Since no red fluorescence reaction positively correlated with benzaldehyde concentration was detected in strain
T1, we process improvement of previous experiments: in the process of integrating HarmOR10 gene to the genome, we
replace the original gene promoter by GAL promoter which has high efficiency, and use galactose in 24-hour
fluorescence intensity test to induce the expression of harmOR10.

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig15.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig16.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/productionsystem.png)

Source of the gene we use

We choose MLPLA(major latex protein-like protein A) and NEPS1(nepetalactone related short chain dehydrogenase 1)
coming from the perennial herb Nepeta cataria to catalyze the conversion from the precursor to nepetalactol and the
conversion from the nepetalactol to nepetalactone.

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig17.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig18.png)

Original strain

The starting strain we used was the AJM-3 strain with a low production of nepetalactol.

Gene integration

Integrating the MLPLA gene on the validated yeast gene integration site int21 to construct a high-yielding-nepetalactol
strain M1.

Integrating the NEPS1 gene on the int20 gene integration site to form a high-yielding- nepetalactone strain M1-N1

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig19.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig20.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig21.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig22.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig23.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig24.png)

The fermentation experiments

In order to verify the products and yields of each strain during the experiment, we performed multiple rounds of
fermentation experiments, the products were detected by GC-MS, the peak times of the nepetalactol and nepetalactone
ester were 8.7 min and 9.4 min.

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig25.png)

Standard curve

Different concentrations of nepetalactol and nepetalactone were used for GC-MS determination to establish a linear
relationship between peak area and product concentration.

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig26.png)
![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig27.png)

Optimization of fermentation time condition

The fermentation experiments of origin strain and M1 strain were carried out, and the concentrations of related
products (nepetalactol or nepetalactone) were measured at 24h, 48h and 72h, respectively. It was found that there was
no significant difference in the concentration of the three time points, so in the subsequent experiments, what’s
more, most of the data measured at 24h were higher than those at the other two time points, so 24h fermentation
was used as the sampling time point in the further experiments.

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig28.png)

We performed multiple rounds of fermentation experiments on the original strain, M1 strain and M1-N1 strain, and
the concentrations of related products in fermentation broth were measured at 24h of fermentation. We found that
the integration of the MLPLA gene greatly increased nepetalactol production, and the integration of the NEPS1 gene
convert almost all the nepetalactol to nepetalactone.

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig29.png)

By-product analysis

From M1 and M1-N1 strains‘ fermentation experiment data, we can find that although the integration of NEPS1 gene
resulted in complete conversion of the nepetalactol, nepetalactone yield is far lower than the nepetalactol yield. This
indicates that the NEPS1 enzyme may produce by-products in the process of catalyzing the nepetalactol conversation.
Thus, we scanned all products of fermentation extract and tried to analyze the possible by-products based on the
map, providing the basis for the further modification of enzyme.

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig30.png)

After comparing the two full-scan maps, we found that the M1-N1 strain have high peaks at around 10.7 min and 11.2
min, which is not found in the maps of M1 strain. We analyzed the two peaks and get the possible by-products
below.

![](https://static.igem.wiki/teams/4177/wiki/wiki/eng/fig31.png)

Means of improvement

Preliminary experiments showed that the catalytic efficiency of NEPS1 enzyme was high, and nepetalactol in M1-N1 strain
were completely converted to nepetalactone, which could not achieve our purpose of regulating the ratio of nepetalactol
and nepetalactone. Therefore, we planned to improve the yield of nepetalactol through metabolic engineering in further
experiments. And the promoter GAL1,10 used by the NEPS1 gene should be replaced with a weaker, regulatable
promoter, such as the CUP1 promoter.

{% endblock %}

{% extends "layout.html" %}
{% block title %}Experiments{% endblock %}
{% block lead %}Describe the research, experiments, and protocols you used in your iGEM project.{% endblock %}
{% block page\_content %}

## What should this page contain?

---

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

## Inspirations

---

* [2019 Nantes](https://2019.igem.org/Team%3ANantes/Experiments)
* [2019 TU Eindhoven](https://2019.igem.org/Team%3ATU_Eindhoven/Experiments)
* [2019 Mingdao](https://2019.igem.org/Team%3AMingdao/Demonstrate)
* [2020 Amsterdam](https://2020.igem.org/Team%3AAmsterdam/Experiments)
* [2020 NCTU Formosa](https://2020.igem.org/Team%3ANCTU_Formosa/Experiments)
* [2020 USAFA](https://2020.igem.org/Team%3AUSAFA/Experiments)

{% endblock %}

{% extends "layout.html" %}
{% block title %}Results{% endblock %}
{% block lead %}You can describe the results of your project and your future plans here.{% endblock %}
{% block page\_content %}

## What should this page contain?

---

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

## Describe what your results mean

---

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

## Project Achievements

---

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

## Inspirations

---

* [2019 Newcastle](https://2019.igem.org/Team%3ANewcastle/Results)
* [2019 Munich](https://2019.igem.org/Team%3AMunich/Results)
* [2019 Tec Chihuahua](https://2019.igem.org/Team%3ATec-Chihuahua/Results)
* [2020 Aalto Helsinki](https://2020.igem.org/Team%3AAalto-Helsinki/Results)
* [2020 GreatBay SCIE](https://2020.igem.org/Team%3AGreatBay_SCIE/Results)
* [2020 Queens Canada](https://2020.igem.org/Team%3AQueens_Canada/Results)

{% endblock %}