iGEM 2014 — Team Korea_U_Seoul

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# Team:Korea U Seoul/Notebook

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2014 IGEM 1st minutes

Meeting

- First meeting with 2014 kuas team members. Discussed about what is kuas, synthetic biology and igem.

Information

- Synthetic biology homepage : <http://syntheticbiology.org>

- Part legistry page : <http://part.igem.org>

Homework

- investigate about subject of2008, 2009 igem teams investigate about subject of 2008, 2009 igem teams

2014 IGEM 2nd minutes

Study of igem 2008~2009 colleges

- Minseob - cell to cell communication

- Outer membrane vesicles (OMV) : gram negative bacteria like E. coli have Outer membrane vesicles (OMV), which can transfer toxin to other bacteria or host strains. Omv is composed of bi-lipid layer with water soluble core, so it can transfer both water soluble materials and lipid soluble materials. Omv is a strong candidate in long-distance messaging.

- Hyeyeong -sensitivity tuners, color generator

- Different color expression by density

- Juyoung –biosensor by density

- Check the expression by density divided strong, wild, weak Lock & key, Jammer

- Heonsong – estimating stress by promoter & microorganism which can produce materials when get some light.

- Formation of hybrid-promoter, light-sensing, biomaterial production

- Jihee- Biothermometer using RNA, not protein

- RNA double helix come untied when increase the heat.

- Kyugin- treat for kidney disease

- using NhaA, Urea sensor

Tips of instrutor

- Categorize the theme.
- Regulate the level.
- Refer other colleges access methods.

Discussion of direction

- Take category- one of food/energy, environment, manufacturing.
- Investigate some ideas food & energy part until 2013.12.23

2014 IGEM 4th minutes

Study presentation

- JUYOUNG

- Siderosphore : Fe3+ absorb, high reducing agent. =>use ocean microbes to greenhouse effect, pathogen control, biofuel

- Degradation Aplatoxin

- MINSEOB

- GABA : neuro-material.

- T3SS(Type 3 secretion system) : related to plant immunity. Mechanism has been found.

- Pathogen microorganism sensor : use metabolic product as marker like enterotoxin or virulence factor

- PCN(antifungal product)

- Shiga toxin producing E. coli : can develop to cell to cell communication. Herein.

- checker of cosmetics: ex) PH sensor

- JIHEE

- dye : E. coli producing indigo

- Pectinase+ beta-chrotin, E. coli producing 3-4 nutrients-> making juice

- HYEYEONG

- bio-filter

- removal odors of food junks : ex)remove pathogenity.

- Soil sensor

Next week

* minseob- GABA, Shiga toxin, components of cosmetics
* jihee- effectiveness of making juice
* hyeyeong- effectiveness of removing bad smells and probability of removing pathogenity
* juyeong- about iron ion, removing ground torpedo microbes

2014 IGEM 5th minutes

About ideas

- JUYOUNG

- extension of siderosphore idea

- MINSEOB

- papers of Sigatoxin

- JIHEE

- papers about pection production

- HYEYEONG

- papers of streptomycetes, actinomycetes

Next week

* kyungwoo- feedback to papers
* others- get feedbacks and more study about each idea.

Schedule

Fix idea until middle of January

Accounts

|  |  |  |  |
| --- | --- | --- | --- |
| In | Korean dollar | Out | Korean dollar |
| 2013 |  | Team registration fee | 300 |
| left | 100 | Participation fee | 250 |
| KOFST | 900 | plane | 1020 |
| CCP | 250 | abode | 360 |
| 2014 |  | Team tea-shirts | 30 |
| CCP | 250 |  |  |
| KOFST | 150 |  |  |
| Self-administration of KU | 60 |  |  |
| Mycroryza | 10 |  |  |
| festival | 30 |  |  |
| fee | 20 |  |  |
| sum | 1860 | sum | 1960 |

2014 IGEM 6th minutes

Presentation of ideas

- JUYOUNG

- Siderophore : absorb iron ion FUR system ) Ferric Uptake Regulator.

- Aflatoxin

- MINSEOB

- Shiga toxin @ shiga toxin producing E. coli: cause hemolytic-uremic syndrome (Proprotein convertases :change proprotein to protein )

- Messages in a bubble : outer membrane vesicle (OMV)

- Collaboration : proprotein caspases (one of serine proteases, subtilisin serine proteases) , send subtilisin to STEC (destroyed shiga toxin will be sent to eukaryotic, but it won’t work. )

- Develop experiments of Paris

- JIHEE

- pectinase production from E. coli

- Cellulase+pectinase-> more degradation of pulp

- Use residuum of fruits by pectinase

- HYEYEONG

- Removing bad smells of food junks

- the main components of bad smells include nitrogen->regulate metabolism

- HYUKJIN

- Cellulolytic *E. coli* : cellulose-> oligo-saccharides-> disaccharide -> glucose(Use ocean microbes which can degrade cellulose to disaccharides, Regulating promoter for find ultimate media.)

- Filamentus bacteria phage

- *C. glutamioum*: agarose fili designing: new microbes, not *E. coli*

Next week

* Proteins which can attach to filamentus bacteria phage

2014 IGEM 7th minutes

Dine together: Le’guette 60500 korean dollars

discussion

- OMV
- Agarose
- Bio informatics
- Micro Dynamics

2014 IGEM 8th minutes

discussion

- OMV dynamics
- Reappearance experiment -OMV
- Targeting -OMV
- Agarase + other enzyme metabolic pathway
- Cellulose degradation –agarose degradation

2014 IGEM 9th minutes

Ideas

- OMV reservation
- Circular per-mutation protein

- advantage : high effectiveness

- high tolerance enzyme : endothermic

- new substrate-specificity expression

Human practice

Apply Univ-expo

Next week

- minseob –study the structure, what kind of protein we will attach

2014 IGEM 10th minutes

NGS

Hyukjjin’s presentation

Enzyme fuel cell

reference

- http://www.sony.net/SonyInfo/csr/SonyEnvironment/technology/bio\_battery2.html
- http://www.nature.com/ncomms/2014/140121/ncomms4026/full/ncomms4026.html
- https://2013.igem.org/Team:Bielefeld-Germany

Apply Univ-expo

Human practice

- Waiting contact from Univ EXPO.

2014 IGEM 12th minutes

Ideas

- Kyungwoo - Microbial behavior control

Next week

- Evolutionary dynamics study

2014 IGEM 13th minutes

Dynamics

- Game theory : what does other man thinks what I do
- Future is the reason of the past.

Two prey diet choice program.

Two resource diet choice program.

Calorie restriction

Agarose + glucose media : showing calorie restriction

Rock scissor paper theory

- (p : secrete toxin, low metabolism ability, r : having toxin resistance , reasonable metabolism ability , s : best metabolism ability among three)
- The system make balance between P, r, s

Circular GFP

Use various microbes

Parasitic microbes

When the circumstance changes, changing itself will have low effectiveness than living in the nature.

2014 IGEM 14th minutes

Subject

- Enzyme whip
- Sortase application
- How to design?
- Agar-metabolic fuel cells
- Microbial behavior control; popular

2014 IGEM 16th minutes

JI HEE-DNA SEQUENCING

- What is DNA sequencing?
- Basic Method

- Maxam-Gilbert sequencing

- Sanger sequencing : chain terminating->Dye-terminator sequencing

- Advanced method

- Shotgun sequencing

- Bridge PCR

- Emulsion PCR : DNA fragments are amplified upon primers attached to beads

- Next generation method

- 454pyrosequencing

- Illumine sequencing : use bridge PCR

- Next next generation sequencing
- Next next next generation sequencing

2014 IGEM 19th minutes

Sponsor

- crowdfunding –research sponsoring site , happybean
- The Korean Federation of science and technology society
- apply for ERA synbio
- CCP-(not settled)

2014 IGEM 20th minutes

Todo List

- airplane& accommodation reservation

- airplane ; H.Y.Choi

- accommodation ; J.H.Park

- group t-shirt
- novel human practices ; each person should propose at least one idea.
- we should get better results than high school iGEM.
- wiki design ; D.H.Kim -complete within August
- poster design ; J.H.Park-complete within August

2014 IGEM 21th minutes

Todo List

- iGEM safety form
- human practice) poster display, facebook, reports
- experiment designing
- new application part !
- group t-shirt
- way to earn money

2014 IGEM 22nd minutes

Todo List

- Attention fee for Giant jamboree
- inquiry of speech in CSIA
- preparing wiki materials
- making material for public relations
- Measurement interlab study
- making projet proposal - microbiology lab of CSBL for high school students

2014 IGEM 23rd minutes

Todo List

- fill in wiki
- making materials for public relations
- CSIA speech
- measurement interlab study-x
- feedback from professor
- present condition of experiment

2014 IGEM 24th minutes

Todo List

- fill in wiki
- making film for crowd funding
- present condition of experiment

2014 IGEM 25th minutes

Todo List

- making film for public relations
- speech in CSIA on August 20th 4p.m.
- present condition of experiment

2014 IGEM 26th minutes

Todo List

- present condition of wiki, public relations, experiment
- approvement of ‘check in’
- human practice-CCP
- resetting timetable
- payment of attention fee in Giant Jamboree
- plan for boston

2014 IGEM 27th minutes

Todo List

- Present condition of Wiki
- Present condition of the Tumblbug
- Present condition of experiments
- apllication of ccp

2014 IGEM 28th minutes

Todo List

- Present condition of Wiki
- Present condition of the Tumblbug
- Present condition of experiments
- delay of ccp
- visa

2014 IGEM 29th minutes

Todo List

- Present condition of Wiki
- Present condition of the Tumblbug
- Present condition of experiments
- delay of ccp
- judging form : https://igem.org/2014\_Judging\_Form
- poster guide line : https://2014.igem.org/Poster\_Guidelines

2014 IGEM 30th minutes

Todo List

- data roaming : egg
- visa : HJY, JKJ
- Wiki page-part check

- judging form

- who did experiment, sponsor, advisor

- safety questions

- team collaborations

- present condition of the tumblbug
- present condition of experiments

|  |  |  |  |  |
| --- | --- | --- | --- | --- |
| [![](https://static.igem.org/mediawiki/2013/f/fe/Spon1.png)](http://www.korea.edu) | [![](https://static.igem.org/mediawiki/2013/1/13/Spon2.png)](http://compbio.korea.ac.kr) | [![](https://static.igem.org/mediawiki/2013/1/1c/Spon3.png)](http://ctl.korea.ac.kr) | [![](https://static.igem.org/mediawiki/2013/8/80/Spon4.png)](http://www.cosmogenetech.com) | [![](https://static.igem.org/mediawiki/2013/e/eb/Spon6.png)](http://www.daeillab.co.kr) |

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