iGEM 2020 — Team BITSPilani-Goa_India

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:BITSPilani-Goa India/Notebook

Notebook | SugarGain | iGEM BITS Goa

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[Sugar**Gain**](https://2020.igem.org/Team%3ABITSPilani-Goa_India)

[ ]

* Project
  + [Description](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Description)
  + [Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Design)
  + [Model](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Model)
  + [Engineering](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Engineering)
  + [Safety](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Safety)
  + [Implementation](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Implementation)
* Human Practices
  + [Human-Centered Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Human_Practices)
  + [Science Communication](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Education)
* Parts
  + [Parts](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Parts)
* Journal
  + [Notebook](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Notebook)
  + [Experiments](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments)
  + [Appendix](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Appendix)
* Team
  + [Team](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Team)
  + [Attributions](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Attributions)
  + [Collaborations](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Collaborations)
* Contribution
  + [Overview](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Contribution)
  + [Software](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Software)
* Entrepreneurship
  + [Entrepreneurship](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Entrepreneurship)
* Judging
  + [Judging](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Judging)
  + [Awards](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Awards)
  + [Judging Form](https://2020.igem.org/Team%3ABITSPilani-Goa_India/https%3A/igem.org/2020_Judging_Form?id=3512)

* [Description](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Description)
* [Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Design)
* [Model](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Model)
* [Engineering](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Engineering)
* [Safety](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Safety)
* [Implementation](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Implementation)

* [Human-Centered Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Human_Practices)
* [Science Communication](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Education)

* [Parts](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Parts)

* [Notebook](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Notebook)
* [Experiments](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments)
* [Appendix](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Appendix)

* [Team](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Team)
* [Attributions](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Attributions)
* [Collaborations](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Collaborations)

* [Overview](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Contribution)
* [Software](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Software)

* [Entrepreneurship](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Entrepreneurship)

* [Judging](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Judging)
* [Awards](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Awards)
* [Judging Form](https://igem.org/2020_Judging_Form?id=3512)

* [Instagram](https://instagram.com/igem_bits)
* [Twitter](https://twitter.com/igembitsgoa)
* Email
* [Sample](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Sample)

* [Acknowledgements](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Acknowledgements)

# Notebook

January 2020February 2020March 2020April 2020May 2020June 2020July 2020August 2020September 2020

## January 2020

# Week 1

* **4th:** We had our first full team meeting!

# Week 2

* **6th - 15th:** Brainstormed about various project ideas with our PI and decided to work on post-harvest losses in sugarcane.
* **15th:** We attended the BITSAA Global Meet that provided us with the opportunity to interact with alumni from our university, pitch our project idea and gain valuable feedback.
* **18th:** We met with Mr Sunil Kumar Ohri from the Sugar Federation in UP.

# Week 3

We conducted our initial growth curve experiments in sugarcane media. We looked at multiple dilutions and clarification methods.

# Week 4

We brainstormed about the growth curve model and completed the experiments.

## February 2020

# Week 1

* **2nd:** We had our first meeting with Varsha Jaisimha (iGEM Ambassador to Asia Pacific). Since we are a first time team, she was instrumental in guiding us on how to get started.

Our team conducted extensive research and literature review. We also began our sponsorship drives and our crowd funding initiatives.

# Week 2

We met our PI to work out finer details about our project and thought about how to incorporate Varsha's initial feedback.

# Week 3

We began our initial PCR of invertase of *Arabidopsis thalliana*.

# Week 4

We were on a break this week due to our midterms.

## March 2020

# Week 1

MIDTERM WEEK!

# Week 2

* **13th:** We began our experiments for capillary action in sugarcane.
* **14th:** Instruction at university was suspended in lieu of the COVID-19 pandemic.

# Week 3

No work could be done as everyone was still adjusting to the new normal.

# Week 4

We had our first virtual team meeting! This meeting was crucial for us to redefine our approach. We formulated a weekly schedule for our team meetings.

We restructured our team into subgroups and decided to meet twice a week for working sessions.

## April 2020

# Week 1

* **1st - 7th:** We had the meetings for the sequences and modelling teams.

# Week 2

We had follow up meetings with Varsha to discuss how to go about our project virtually.

# Week 3

We started designing the Atmosphere Regulated Killswitch (BBa\_K3512042). We began formulating the design of the model for the killswitch.

# Week 4

Conducted literature review for the pFruB-Cra module and the kill switch to determine a few parameters.

## May 2020

# Week 1

* We conducted docking studies of anti-invertase and invertase.
* We also modified our approach in the *in silico* modelling of our pFruB-Cra system and kill switch.
* Our wet lab team began the construction of the pFruB-Cra sequences.
* We searched for contacts of farmers to augment our human practices approach.

# Week 2

We continued working on tasks from the previous week and set up meetings with farmers in Gulbarga and Mandya.

# Week 3

* We met the farmers from Gulbarga and Mandya.

# Week 4

We thought about how we could implement their suggestions in our design.

## June 2020

# Week 1

* We created the initial model of our kill switch and growth curve. We also sent our biobrick sequences to our PI for verification.
* We met the Chief Chemist from the Kichha Sugar Company.

# Week 2

* 13th: We began our collaboration with Abhigyaan — a student run organization at BITS Goa.
* 19th: We met the iGEM OSU team regarding biocontainment and kill switch modelling.

# Week 3

* 23rd: We had our first meeting with the University of Rochester and IITM and the Accessibility Initiative was started!
* We made an initial prototype for WikiSync.

# Week 4

* 30th: We met a farmer from the Tamil Nadu belt.
* Set up Travis for CI/CD for development of WikiSync.

## July 2020

# Week 1

* 4th: We met with the iGEM Groningen Team and learnt more about their project.
* We started formulating a plan for the modelling of the biosensor.
* We also had a meeting with our secondary PI to discuss various experimental designs that can be implemented.
* Reorganized WikiSync codebase to split into modules for easier debugging.
* We started working on our wiki and the Wiki Starter Pack.

# Week 2

* 14th: We met with Andre Hermann regarding the sugar industry in Brazil and guidance on policy making. We also released our project logo!
* 15th: We started our Humans of iGEM initiative.
* We incorporated feedback from iGEM Groningen into our kill switch model.
* We wrote file classes, a basic HTML parser and started building the upload map functionality for WikiSync.
* We added automatic citations functionality to the Wiki Starter Pack.

# Week 3

* 22nd: We met with Dr Bejoy Thomas about our economic modelling.
* 25th: We hosted the first ever BioSummit!
* Added a CSS parser to WikiSync.
* Start writing tests for WikiSync.
* Added some more features to Starter Pack.

# Week 4

* 31st: The Indian teams got together for the first All India iGEM Meet!
* Start writing documentation for WikiSync and Starter Pack along with more tests.

## August 2020

# Week 1

* We continued consulting with iGEM Groningen on our killswitch model and and began rethinking our approach. We also completed our biosensor model.
* The required experimental protocols were finalised.
* More tests and documentation for WikiSync.
* Prepared Starter Pack for release during the Global Meetup.

# Week 2

* Our college reopened for the fall semester.
* We practiced with iGEM Rochester for our Global Meetup sessions and recorded them.

# Week 3

* 19th - 22nd: Our team hosted sessions during the Global Meetup.
* We wrote usage guides for WikiSync along with some more features.

# Week 4

* 30th: We had our first meeting with Synbio for Everyone where we discussed about our Accessibility initiative.
* We restructured our economic model based on the feedback recieved during our AIIM presentation.

## September 2020

# Week 1

* We completed our model for the inoculant as well as auxotroph design.
* We began designing the process flow diagram and industrial application of our product.
* 9th: We finalised our wiki illustrations and design!

# Week 2

* We began brainstorming on ideas for our promotional video and reached out to the Mime Club on campus.
* We wrapped-up the Language project.

# Week 3

* This week was spent finalising our safety forms after feedback from PI.
* We also submitted our biosafety proposal to the Biosafety Committee on campus.

# Week 4

MIDTERM WEEK!

## October 2020

# Week 1

We started writing first drafts for the content on our wiki and worked on its design.

# Week 2

* We completed animations for our homepage and added all the pages as per the Judging Form.

# Week 3

* We geared up for the wiki freeze and started working on the presentation video.

# Week 4

WIKI FREEZE!

[Home](https://2020.igem.org/Team%3ABITSPilani-Goa_India)[Experiments](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments)

Project

* [Description](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Description)
* [Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Design)
* [Model](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Model)
* [Engineering](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Engineering)
* [Safety](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Safety)
* [Implementation](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Implementation)

Human Practices

* [Human-Centered Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Human_Practices)
* [Science Communication](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Education)

Parts

* [Parts](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Parts)

Journal

* [Notebook](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Notebook)
* [Experiments](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments)
* [Appendix](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Appendix)

Team

* [Team](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Team)
* [Attributions](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Attributions)
* [Collaborations](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Collaborations)

Contribution

* [Overview](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Contribution)
* [Software](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Software)

Entrepreneurship

* [Entrepreneurship](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Entrepreneurship)

Judging

* [Judging](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Judging)
* [Awards](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Awards)
* [Judging Form](https://2020.igem.org/Team%3ABITSPilani-Goa_India/https%3A/igem.org/2020_Judging_Form?id=3512)

iGEM BITS Goa

Birla Institute of Technology and Science, Pilani

K K Birla Goa Campus

NH-17B, Sancoale, Goa, India

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:BITSPilani-Goa India/Engineering

Engineering | SugarGain | iGEM BITS Goa

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[Sugar**Gain**](https://2020.igem.org/Team%3ABITSPilani-Goa_India)

[ ]

* Project
  + [Description](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Description)
  + [Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Design)
  + [Model](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Model)
  + [Engineering](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Engineering)
  + [Safety](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Safety)
  + [Implementation](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Implementation)
* Human Practices
  + [Human-Centered Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Human_Practices)
  + [Science Communication](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Education)
* Parts
  + [Parts](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Parts)
* Journal
  + [Notebook](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Notebook)
  + [Experiments](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments)
  + [Appendix](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Appendix)
* Team
  + [Team](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Team)
  + [Attributions](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Attributions)
  + [Collaborations](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Collaborations)
* Contribution
  + [Overview](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Contribution)
  + [Software](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Software)
* Entrepreneurship
  + [Entrepreneurship](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Entrepreneurship)
* Judging
  + [Judging](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Judging)
  + [Awards](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Awards)
  + [Judging Form](https://2020.igem.org/Team%3ABITSPilani-Goa_India/https%3A/igem.org/2020_Judging_Form?id=3512)

* [Description](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Description)
* [Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Design)
* [Model](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Model)
* [Engineering](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Engineering)
* [Safety](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Safety)
* [Implementation](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Implementation)

* [Human-Centered Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Human_Practices)
* [Science Communication](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Education)

* [Parts](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Parts)

* [Notebook](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Notebook)
* [Experiments](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments)
* [Appendix](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Appendix)

* [Team](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Team)
* [Attributions](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Attributions)
* [Collaborations](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Collaborations)

* [Overview](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Contribution)
* [Software](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Software)

* [Entrepreneurship](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Entrepreneurship)

* [Judging](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Judging)
* [Awards](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Awards)
* [Judging Form](https://igem.org/2020_Judging_Form?id=3512)

* [Instagram](https://instagram.com/igem_bits)
* [Twitter](https://twitter.com/igembitsgoa)
* Email
* [Sample](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Sample)

* [Acknowledgements](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Acknowledgements)

# Engineering

While designing SugarGain, we had to keep various considerations in mind. The initial idea was broken down into a set of problem statements and these formed the major pillars of our project. The following diagrams illustrate the flow of our thought as we tried to solve these problems to the best of our ability. We also illustrate how we modelled our project and its interconnectedness with the experiments we designed to complement the modelled data. Furthermore, we have taken into consideration the different outcomes that these experiments might lead to and planned accordingly. Due to unavailability of lab access this year, we plan to finish these experiments in phase 2.

For more details about these experiments, please see our [Experiments](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments) page.

# pFruB-Cra System

## Problem Statement

Our project aims to reduce the activity of invertase, an enzyme that converts useful sucrose into profitless glucose and fructose. Since this process was important to the life cycle of the plant, it wasn’t practical to genetically re-engineer sugarcane cells itself. The most harm this enzyme causes is post-harvest and hence, that seemed like the most logical time period for us to target.

## Designing Process

1

After surveying publications on invertases, we came across a group of proteins called anti-invertases/invertase inhibitor which regulate invertase and control sugar hydrolysis.

3

We also discovered that anti-invertase has quite a small half-life. This meant that the invertase would have to be secreted at the site of action (i.e., inside the sugarcane).

2

To decrease the nutrient load on our bacterial chassis and have a controlled release of our protein, we decided on a biosensor mechanism.

4

Since the concentration of fructose was a more or less direct result of invertase activity, we wanted to calibrate a self-regulatory mechanism that modulated the secretion of the anti-invertase in tandem with the concentration of fructose in the medium. We were able to achieve that by using a pFruB-Cra system.

5

Using these parts, we constructed our fructose regulated anti invertase.

## Modelling

Biosensor will simulate output of invertase inhibitor for particular levels of fructose in the environment and will also link the promoter activity to a measurable output.

## Experimental Design

Due to the global pandemic, we were unable to complement our modelling with experimental results. However, we prepared and planned for these experiments to strengthen our project design and help the next team carry out our project to completion.

**Homology Modelling and Docking**

We tried to get an insight into the protein-protein interaction through modelling of anti-invertase with invertase.

**Homology Modelling**

We tried to get an insight into the protein-DNA interaction through homology modelling of FruR.

**Docking studies of FruR with F-1-P**

Since FruR and its interactions are not extensively characterised, we had to conduct molecular docking studies in order to elucidate the interactions between FruR and fructose.

Identify the optimum Kd value for the system to work

In phase 2, prediction of overall efficiency of the pFruB-Cra system and the amount of inoculant required per sugarcane using information from experiments.

Anti-Invertase Assay to characterise the functioning of anti-invertase.

FruR Specificity Experiment

pFruB-FruR Characterisation

Gel shift assay to experimentally determine Kd value of pFruB.

Site-directed mutagenesis of the pFruB to bring the Kd value to the one established through the model

if inadequate

# Atmosphere Regulated Killswitch

## Problem Statement

Given the consumable nature of our product, we had to come up with a robust killswitch that was capable of effectively terminating the bacteria taking into account the toxicity of killswitch with respect to the humans.

## Designing Process

1

First, we ascertained the trigger to be the swift and abrupt change in atmospheric conditions (anaerobic inside the sugarcane stem and aerobic outside)

2

Second, we found a toxin-antitoxin pair that was quite fatal to bacteria but did not affect human cells.

3

After an extensive search of the registry, we found the FNR promoter that was only activated in anaerobic conditions.

4

Using these parts, we designed our killswitch genetic circuit.

## Modelling

We focussed on modelling the behaviour of the circuit to the change in concentration of oxygen in the atmosphere. We further predicted the response time for the same.

## Experimental Design

Due to the global pandemic, we were unable to complement our modelling with experimental results. However, we prepared and planned for these experiments to strengthen our project design and help the next team carry out our project to completion.

Create a persister cell model in phase 2

Differential expression of the antitoxin in the presence and absence of oxygen

Time required for the buildup of toxin concentration sufficient to restrict bacterial growth

Identify the optimum Kd value for the system to work

Assay to check if the killswitch functions as intended

Killswitch response time assay to ascertain the time required after exposure for the cell to succumb

Gel shift assay to experimentally determine Kd value of the FNR promoter

Site-directed mutagenesis of the FNR promoter to bring the Kd value to the one established through the model

pH dependency of the FNR promoter (due to the fluctuation in pH in the sugar extraction process)

if high cell viability

if not quick enough

if inadequate Kd value

# Polymer Inoculant

## Problem Statement

Since our product includes live bacteria, we wanted to ensure that the product had a long shelf life. After consulting farmers in various belts across India, we also came across the need for the product to be organic, non-toxic and cheap.

## Designing Process

1

We started researching polymer-based inoculants that had longevity and were organic in nature.

2

We determined the optimal inoculant composition of a Carboxymethylcellulose-based polymer with bentonite suspending agent and sorbic acid preservative.

3

We optimized the viscosity to minimize the inoculant's toxicity, and ensured that it is close to that of water for efficient transport.

4

This inoculant would be supplied to the farmer and can be used through an injector mechanism. In this manner the sugarcane's inherent transport mechanism due to transpirational pull is leveraged for the uniform distribution of the bacteria through the plant.

## Modelling

Keeping in mind the concerns of the sugarcane farmers across major Indian belts,we devised ways to optimise the features of the polymer inoculant.

## Experimental Design

Due to the global pandemic, we were unable to complement our modelling with experimental results. However, we prepared and planned for these experiments to strengthen our project design and help the next team carry out our project to completion.

Kinematic Viscosity model and Toxicity model

Proposed implementation and economic scalability model

Removal of chemical residues of polymer inoculant during processing of sugarcane juice

Validating toxicity model

Capillary action experiment will help with delivery mechanism model in proposed implementation

[Model](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Model)[Safety](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Safety)

Project

* [Description](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Description)
* [Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Design)
* [Model](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Model)
* [Engineering](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Engineering)
* [Safety](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Safety)
* [Implementation](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Implementation)

Human Practices

* [Human-Centered Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Human_Practices)
* [Science Communication](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Education)

Parts

* [Parts](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Parts)

Journal

* [Notebook](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Notebook)
* [Experiments](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments)
* [Appendix](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Appendix)

Team

* [Team](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Team)
* [Attributions](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Attributions)
* [Collaborations](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Collaborations)

Contribution

* [Overview](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Contribution)
* [Software](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Software)

Entrepreneurship

* [Entrepreneurship](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Entrepreneurship)

Judging

* [Judging](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Judging)
* [Awards](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Awards)
* [Judging Form](https://2020.igem.org/Team%3ABITSPilani-Goa_India/https%3A/igem.org/2020_Judging_Form?id=3512)

iGEM BITS Goa

Birla Institute of Technology and Science, Pilani

K K Birla Goa Campus

NH-17B, Sancoale, Goa, India

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:BITSPilani-Goa India/Experiments

Experiments | SugarGain | iGEM BITS Goa

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[Sugar**Gain**](https://2020.igem.org/Team%3ABITSPilani-Goa_India)

[ ]

* Project
  + [Description](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Description)
  + [Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Design)
  + [Model](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Model)
  + [Engineering](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Engineering)
  + [Safety](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Safety)
  + [Implementation](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Implementation)
* Human Practices
  + [Human-Centered Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Human_Practices)
  + [Science Communication](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Education)
* Parts
  + [Parts](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Parts)
* Journal
  + [Notebook](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Notebook)
  + [Experiments](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments)
  + [Appendix](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Appendix)
* Team
  + [Team](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Team)
  + [Attributions](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Attributions)
  + [Collaborations](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Collaborations)
* Contribution
  + [Overview](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Contribution)
  + [Software](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Software)
* Entrepreneurship
  + [Entrepreneurship](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Entrepreneurship)
* Judging
  + [Judging](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Judging)
  + [Awards](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Awards)
  + [Judging Form](https://2020.igem.org/Team%3ABITSPilani-Goa_India/https%3A/igem.org/2020_Judging_Form?id=3512)

* [Description](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Description)
* [Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Design)
* [Model](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Model)
* [Engineering](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Engineering)
* [Safety](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Safety)
* [Implementation](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Implementation)

* [Human-Centered Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Human_Practices)
* [Science Communication](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Education)

* [Parts](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Parts)

* [Notebook](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Notebook)
* [Experiments](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments)
* [Appendix](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Appendix)

* [Team](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Team)
* [Attributions](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Attributions)
* [Collaborations](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Collaborations)

* [Overview](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Contribution)
* [Software](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Software)

* [Entrepreneurship](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Entrepreneurship)

* [Judging](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Judging)
* [Awards](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Awards)
* [Judging Form](https://igem.org/2020_Judging_Form?id=3512)

* [Instagram](https://instagram.com/igem_bits)
* [Twitter](https://twitter.com/igembitsgoa)
* Email
* [Sample](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Sample)

* [Acknowledgements](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Acknowledgements)

# Experiments

[![](https://static.igem.org/mediawiki/2020/d/db/T--BITSPilani-Goa_India--img--Design--AI.gif)

## ExperimentspFruB-Cra System](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments/pFruB-Cra_System)

[![](https://static.igem.org/mediawiki/2020/0/05/T--BITSPilani-Goa_India--img--Landing--Kill_Switch.gif)

## ExperimentsKill Switch](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments/Kill_Switch)

[![](https://static.igem.org/mediawiki/2020/7/79/T--BITSPilani-Goa_India--img--Landing--Inoculant.jpg)

## ExperimentsInoculant](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments/Inoculant)

[![](https://static.igem.org/mediawiki/2020/1/13/T--BITSPilani-Goa_India--img--Landing--Auxotroph.png)

## ExperimentsOther](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments/Other)

[![](https://static.igem.org/mediawiki/2020/d/d2/T--BITSPilani-Goa_India--img--Landing--Kill_Switch.jpeg)

## ExperimentsProtocols](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments/Protocols)

[Notebook](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Notebook)[Appendix](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Appendix)

Project

* [Description](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Description)
* [Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Design)
* [Model](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Model)
* [Engineering](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Engineering)
* [Safety](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Safety)
* [Implementation](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Implementation)

Human Practices

* [Human-Centered Design](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Human_Practices)
* [Science Communication](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Education)

Parts

* [Parts](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Parts)

Journal

* [Notebook](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Notebook)
* [Experiments](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Experiments)
* [Appendix](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Appendix)

Team

* [Team](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Team)
* [Attributions](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Attributions)
* [Collaborations](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Collaborations)

Contribution

* [Overview](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Contribution)
* [Software](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Software)

Entrepreneurship

* [Entrepreneurship](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Entrepreneurship)

Judging

* [Judging](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Judging)
* [Awards](https://2020.igem.org/Team%3ABITSPilani-Goa_India/Awards)
* [Judging Form](https://2020.igem.org/Team%3ABITSPilani-Goa_India/https%3A/igem.org/2020_Judging_Form?id=3512)

iGEM BITS Goa

Birla Institute of Technology and Science, Pilani

K K Birla Goa Campus

NH-17B, Sancoale, Goa, India

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:BITSPilani-Goa India/Results

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)