iGEM 2011 — Team IIT_Madras

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# Team:IIT Madras/Notebook

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| Contents   * [1 Notebook](#Notebook)   + [1.1 Week 1](#Week_1)   + [1.2 Week 2](#Week_2)   + [1.3 Week 3](#Week_3)   + [1.4 Week 4](#Week_4)   + [1.5 Week5](#Week5)   + [1.6 Week6](#Week6)   + [1.7 Week7](#Week7)   + [1.8 Week8](#Week8)   + [1.9 Week9](#Week9)   + [1.10 Week10](#Week10)   + [1.11 Week11](#Week11) |

# Notebook

## Week 1

- Metabolic simulation and modelling of increase in proton flux on addition of Proteorhodopsin- Literature survey on expression of Proteorhodopsin in E.coli

    ## Week 2

    - Minimal media characterization of DH5-alpha- Modelling survival of PR transformed E.coli cells v/s wild type E.coli- Literature survey on increase in recombinant protein expression in PR transformed cells

          ## Week 3

          - Obtained Proteorhodopsin gene and Beta-Dioxygenase in PKJ-900 from Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea.- Designing the incubator for experimental studies with Proteorhodopsin- Experimental analysis of growth rates of PR transformed E.coli and DH5-alpha in presence of ETC-Inhibitor Azide

                ## Week 4

                - Standardizing the protocol for digestion of pKJ900- Designing RFP generator for promoter studies

                    ## Week5

                    - Generating the PcstA\_RFP Generator- Synthesizing the T7\_RFP Generator

                        ## Week6

                        - Characterizing PcstA\_RFP Generator- Designing the promoters for Proteorhodopsin gene- Codon optimization of Proteorhodopsin gene

                              ## Week7

                              - Designing the OmpR Promoter\_RFP Generator- Designing the PcstA\_Luciferase Generator- Digestion of Promoters

                                    ## Week8

                                    - Transformation of Promoters- Standardizing the protocols for transformation of proteorhodopsin- Designing the Proteorhodopsin Generator

                                          ## Week9

                                          - Preparation for Presentation at IITM Research Expo- Lecture preparation on synthetic biology- Characterizing growth behaviour of Proteorhodopsin transformed cells on various minimal media under light/dark conditions.

                                                ## Week10

                                                - Expression studies of the recombinant protein in proteorhodopsin transformed E.coli- Presentation at IITM Research Expo

                                                    ## Week11

                                                    - Wiki designing

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