iGEM 2015 — Team Tec-Chihuahua

# Team:Tec-Chihuahua/Notebook

Carbon carriers

* [Home](https://2015.igem.org/Team%3ATec-Chihuahua)
* [Team](https://2015.igem.org/Team%3ATec-Chihuahua/Attributions)
* [Project](https://2015.igem.org/Team%3ATec-Chihuahua/Project)
* [NoteBook](https://2015.igem.org/Team%3ATec-Chihuahua/Notebook)
* [Parts](https://2015.igem.org/Team%3ATec-Chihuahua/Parts)
* [Safety](https://2015.igem.org/Team%3ATec-Chihuahua/Safety)
* [Modeling](https://2015.igem.org/Team%3ATec-Chihuahua/Modeling)
* [Human Practices](https://2015.igem.org/Team%3ATec-Chihuahua/Practices)
* [Collaboration](https://2015.igem.org/Team%3ATec-Chihuahua/Collaborations)
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# Notebook

* [Protocols](#protocols)
* [Journal](#journal)

# Protocols

## CNT

* [Synthesis and Purification CNT![](https://static.igem.org/mediawiki/2015/3/37/Tec-chi-pdfico.png)](https://static.igem.org/mediawiki/2015/5/52/Tec-chihuahua-notebook-synthesisandpurification.pdf)
* [Characterization CNT![](https://static.igem.org/mediawiki/2015/3/37/Tec-chi-pdfico.png)](https://static.igem.org/mediawiki/2015/4/4d/Tec-chihuahua-notebook-characterizationcnt.pdf)

## Oocytes

* [![](https://static.igem.org/mediawiki/2015/3/37/Tec-chi-pdfico.png)Vacuuming, selection and Maduration](https://static.igem.org/mediawiki/2015/f/f9/Tec-chihuahua-notebook-vacuuming.pdf)
* [![](https://static.igem.org/mediawiki/2015/3/37/Tec-chi-pdfico.png)Competent Cells](https://static.igem.org/mediawiki/2015/9/94/Tec-chihuahua-notebook-competentcells.pdf)
* [![](https://static.igem.org/mediawiki/2015/3/37/Tec-chi-pdfico.png)Cell Transformation](https://static.igem.org/mediawiki/2015/4/49/Tec-chihuahua-notebook-celltransformation.pdf)
* [![](https://static.igem.org/mediawiki/2015/3/37/Tec-chi-pdfico.png)Interaction and Fixation](https://static.igem.org/mediawiki/2015/4/44/Tec-chihuahua-notebook-interaction%26fixation.pdf)
* [![](https://static.igem.org/mediawiki/2015/3/37/Tec-chi-pdfico.png)DAPI](https://static.igem.org/mediawiki/2015/9/96/Tec-chihuahua-notebook-DAPI.pdf)

## Parts

* [Assembling![](https://static.igem.org/mediawiki/2015/3/37/Tec-chi-pdfico.png)](http://parts.igem.org/Help%3AProtocols/3A_Assembly)
* [Digestion![](https://static.igem.org/mediawiki/2015/3/37/Tec-chi-pdfico.png)](http://parts.igem.org/Help%3AProtocols/Restriction_Digest)
* [MiniPrep![](https://static.igem.org/mediawiki/2015/3/37/Tec-chi-pdfico.png)](https://static.igem.org/mediawiki/2015/b/b2/Tec-chihuahua-notebook-miniprep.pdf)

## Plants

* [![](https://static.igem.org/mediawiki/2015/3/37/Tec-chi-pdfico.png)Plants Transfection](https://static.igem.org/mediawiki/2015/5/55/Tec-chihuahua-notebook-plants.pdf)

# Journal

### **Feb** / 26 / 2015

First meeting of the team. We talked about the proyect and started generating ideas.

### **Mar** / 4 / 2015

Choice of the project.

### **Mar** / 14 / 2015

We won the Entrepreneurship Contest of the Nucleum Congress in Monterrey.

### **Mar** / 19 / 2015

Team Meeting.

### **Mar** / 26 / 2015

Team promotional photo.

![](https://static.igem.org/mediawiki/2015/7/7c/Tec-chihuahua-notebook-march04.jpeg)

### **Abr** / 2 / 2015

Team Meeting

### **Abr** / 9 / 2015

We presented our ideas to experts of the Centro de Investigación en Materiales Avanzados (CIMAV), they seem interested on the Project and gave us their support.

![](https://static.igem.org/mediawiki/2015/f/fa/Tec-chihuahua-notebook-abril09.jpeg)

### **Abr** / 17 / 2015

We went to the local TV Show “Emma de la O” to talk about the biotechnology and our project.

![](https://static.igem.org/mediawiki/2015/d/d8/Tec-chihuahua-notebook-abril17.jpeg)

### **Abr** / 20 / 2015

Synthesis of the NP-CNT. We began the synthesis of the nanotubes at CIMAV.

### **May** / 12 - 15 / 2015

Purification of the NP-CNT

![](https://static.igem.org/mediawiki/2015/e/e4/Tec-chihuahua-notebook-may12.jpeg)

### **May** / 20 / 2015

Filtration of the P-CNT

### **May** / 21 / 2015

Preparation of Luria Bertani Broth for E. coli

### **May** / 22 / 2015

We finished the filtration of the nanotubes

Plasmid extraction of E. coli

![](https://static.igem.org/mediawiki/2015/9/9d/Tec-chihuahua-notebook-may22.jpeg)

### **May** / 25 / 2015

We measured the purity of plasmid using the NanoDrop 2000

![](https://static.igem.org/mediawiki/2015/8/8f/Tec-chihuahua-notebook-may25.jpeg)

### **May** / 27 / 2015

Team meeting to discuss about the wiki

### **May** / 28 / 2015

Inventory of team´s material and lab cleanup.

### **Jun** / 1 / 2015

Preparation of Luria Bertani agar.

Team meeting for fund collection.

Sterilizing of work solutions.

We began to dry the P-CNT

### **Jun** / 3 / 2015

We finished drying the nanotubes.

Preparation of PBS

### **Jun** / 8 / 2015

We began the work with the bovine oocytes at the Facultad de Zootecnia (School of Animal Husbandry) of the Universidad Autónoma de Chihuahua (UACH).

![](https://static.igem.org/mediawiki/2015/4/44/Tec-chihuahua-notebook-june08.jpeg)

### **Jun** / 9 / 2015

Arriving of the Florescein Diacetate.

Oocytes extraction and selection.

The maduration of the viables oocytes began.

![](https://static.igem.org/mediawiki/2015/0/01/Tec-chihuahua-notebook-june09.jpeg)

### **Jun** / 10 / 2015

We began the CNT-Fluorescein functionalization.

Cleaning and picking of the oocytes.

![](https://static.igem.org/mediawiki/2015/1/13/Tec-chihuahua-notebook-june10.jpeg)

### **Jun** / 11 / 2015

We finished the CNT functionalization with fluorescein.

![](https://static.igem.org/mediawiki/2015/3/3c/Tec-chihuahua-notebook-june11.jpeg)

### **Jun** / 15 / 2015

Pins Ceremony

![](https://static.igem.org/mediawiki/2015/d/dc/Tec-chihuahua-notebook-june15.jpeg)

### **Jun** / 16 / 2015

F-NTC / Oocytes interaction. We failed

### **Jun** / 18 / 2015

Purification of the Second batch of CNT.

### **Jun** / 22 / 2015

Preparation of the stocks and the media for the handling and maduration of oocytes.

### **Jun** / 24 / 2015

Oocytes extraction and selection.

The maduration of the viables oocytes began.

![](https://static.igem.org/mediawiki/2015/a/a7/Tec-chihuahua-notebook-june24.jpeg)

### **Jun**/ 25 / 2015

Cleaning and picking of the oocytes.

F-NTC / Oocytes interaction.

Filtration of the second group of nanotubes

![](https://static.igem.org/mediawiki/2015/3/3d/Tec-chihuahua-notebook-june25.jpeg)

### **Jun** / 26 / 2015

“About our lab” and “About our proyect” Questionnaires.

### **Jun** / 29 / 2015

Team meeting.

Observation of functionalized oocytes with confocal microscope.

![](https://static.igem.org/mediawiki/2015/c/c5/Tec-chihuahua-notebook-june29.jpeg)

### **Jun** / 30 / 2015

Filtration of the CNT.

![](https://static.igem.org/mediawiki/2015/2/25/Tec-chihuahua-notebook-june30.jpeg)

### **Jul** / 1 / 2015

We finished the filtration of the second batch of CNT.

### **Jul** / 2 / 2015

Drying of the P-CNT.

Oocytes extraction and selection.

Preparation of work solutions.

### **Jul** / 3 / 2015

Preparation of work solutions.

### **Jul** / 4 / 2015

F-NTC / Oocytes interaction and staining of the oocytes with DAPI.

### **Jul** / 6 /2015

Team meeting

### **Jul** / 7 / 2015

Presentation to high school students about Synthetic Biology for the Human Practices.

![](https://static.igem.org/mediawiki/2015/e/e8/Tec-chihuahua-notebook-july07.jpeg)

### **Jul** / 8 / 2015

Oocytes extraction and selection for maduration

We fabricated glass pipettes

Wiki meeting with Sammy.

### **Jul** / 9 / 2015

Cleaning and picking of the oocytes.

### **Jul** / 10 / 2015

F-NTC / Oocytes interaction and staining of the oocytes with DAPI.

![](https://static.igem.org/mediawiki/2015/7/7c/Tec-chihuahua-notebook-july10.jpeg)

### **Jul** / 13 / 2015

We took a Biosafety Course for the Safety requirements.

Wiki meeting with Sammy

### **Jul** / 15 / 2015

Team meeting.

![](https://static.igem.org/mediawiki/2015/8/88/Tec-chihuahua-notebook-july15.jpeg)

### **Jul** / 16 / 2015

Wiki meeting with Sammy.

### **Jul** / 17 / 2015

Preparation of work solutions.

### **Jul** / 20 / 2015

Meeting with Dr. Quintín Rascon to planning the work on  N. tabacum.

### **Jul** / 21 / 2015

We started Functionalization with Fluorescein of the second batch of P-CNT.

Oocytes extraction and selection for maduration.

### **Jul** / 22 / 2015

We finished the CNT-functionalization.

### **Jul** / 23 / 2015

Preparation of solutions.

Interaction of the oocytes with the F-CNT (both of the batches), previously fixed with paraformaldehyde.

### **Jul** / 24 / 2015

Team meeting

![](https://static.igem.org/mediawiki/2015/5/58/Tec-chihuahua-notebook-july24.jpeg)

### **Jul** / 27 - 31 / 2015

Research and documentary work.

Working on the wiki content.

![](https://static.igem.org/mediawiki/2015/d/dd/Tec-chihuahua-notebook-july27.jpeg)

### **Aug** / 03 / 2015

Wiki Content work

Team meating

### **Aug** / 04 - 05 / 2015

Wiki meeting with Sammy

### **Aug** / 06 / 2015

we grew E. coli TOP10 on LB brith and incubated it on shaker

### **Aug** / 07 / 2015

Sterilizing solutions

Aliquots of E. coli and glycerol were prepared

### **Aug** / 10 / 2015

Team meeting

### **Aug** / 11 / 2015

Preparation of Solutions

Preparation of LB Broth

The optical density of TOP10 was measured

### **Aug** / 12 - 14 / 2015

Biobricks cloning started: We failed on cell transformation

### **Sep** / 04 - 18 / 2015

Lab work with the biobricks

Fast and Furiously Finishing the Wiki

Last details of presentation and poster

Making of promotional video.

![](https://static.igem.org/mediawiki/2015/3/35/Tec-chihuahua-notebook-sept02.jpeg)

# Months of work finally come to end!

Address

Av. Heróico Colegio Militar 4700 Col. Nombre de Dios, Zip Code: 31300

Phone Number

+52 (614) 439 5000 (Ext. 3009)

Email

igem\_chih@outlook.com