iGEM 2018 — Team SHPH-Shanghai

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# Team:SHPH-Shanghai/Notebook

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[HOME](https://2018.igem.org/Team%3ASHPH-Shanghai)

[TEAM](https://2018.igem.org/Team%3ASHPH-Shanghai/Team)

[EXPERIMENT](https://2018.igem.org/Team%3ASHPH-Shanghai/Experiments)
[COLLABORATIONS](https://2018.igem.org/Team%3ASHPH-Shanghai/Collaborations)
[ATTRIBUTIONS](https://2018.igem.org/Team%3ASHPH-Shanghai/Attributions)
[SAFETY](https://2018.igem.org/Team%3ASHPH-Shanghai/Safety)
[HUMAN PRACTICES](https://2018.igem.org/Team%3ASHPH-Shanghai/Human_Practices)
[PARTS](https://2018.igem.org/Team%3ASHPH-Shanghai/Parts)
[JUDGING FORM](https://igem.org/2018_Judging_Form?team=SHPH-Shanghai)

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)


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# Team:SHPH-Shanghai/Experiments

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[HOME](https://2018.igem.org/Team%3ASHPH-Shanghai)

[TEAM](https://2018.igem.org/Team%3ASHPH-Shanghai/Team)

[EXPERIMENT](https://2018.igem.org/Team%3ASHPH-Shanghai/Experiments)
[COLLABORATIONS](https://2018.igem.org/Team%3ASHPH-Shanghai/Collaborations)
[ATTRIBUTIONS](https://2018.igem.org/Team%3ASHPH-Shanghai/Attributions)
[SAFETY](https://2018.igem.org/Team%3ASHPH-Shanghai/Safety)
[HUMAN PRACTICES](https://2018.igem.org/Team%3ASHPH-Shanghai/Human_Practices)
[PARTS](https://2018.igem.org/Team%3ASHPH-Shanghai/Parts)
[JUDGING FORM](https://igem.org/2018_Judging_Form?team=SHPH-Shanghai)

EXPERIMENT

[# BACKGROUND](https://2018.igem.org/Team%3ASHPH-Shanghai/Description)
[# DESIGN](https://2018.igem.org/Team%3ASHPH-Shanghai/Design)
[# INTERLAB

[# RESULTS](https://2018.igem.org/Team%3ASHPH-Shanghai/Results)](https://2018.igem.org/Team%3ASHPH-Shanghai/InterLab)

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# Team:SHPH-Shanghai/Results

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[HOME](https://2018.igem.org/Team%3ASHPH-Shanghai)

[TEAM](https://2018.igem.org/Team%3ASHPH-Shanghai/Team)

[EXPERIMENT](https://2018.igem.org/Team%3ASHPH-Shanghai/Experiments)
[COLLABORATIONS](https://2018.igem.org/Team%3ASHPH-Shanghai/Collaborations)
[ATTRIBUTIONS](https://2018.igem.org/Team%3ASHPH-Shanghai/Attributions)
[SAFETY](https://2018.igem.org/Team%3ASHPH-Shanghai/Safety)
[HUMAN PRACTICES](https://2018.igem.org/Team%3ASHPH-Shanghai/Human_Practices)
[PARTS](https://2018.igem.org/Team%3ASHPH-Shanghai/Parts)
[JUDGING FORM](https://igem.org/2018_Judging_Form?team=SHPH-Shanghai)

[## 1.Result & Analysis](#ra)
[## 2.Gene Extraction](#ge)
[## 3.PCR](#pcr)

Result and analysis

(1) Result of the Pre-experiment

In each plate, from left to right, the pH value for each hole is respectively 1,3,5,7.

![](https://static.igem.org/mediawiki/2018/b/b5/T--SHPH-Shanghai--boli.png)

![](https://static.igem.org/mediawiki/2018/f/fc/T--SHPH-Shanghai--san.png)

(2) Analysis of the Pre-experiment

From both the photography of the 96-microwell plate and the value of OD in each hole, we can observe a significant relevance between the level of biofilm degradation and acidic culturing environment. In the graph, biofilms under condition of pH=1 and pH=3 were very thin and hard to observe, which is consistent with the OD value. On the other hand, biofilms under the condition of pH=5 and 7, the degradation hardly took place.

From the perspective of designing the investigating subject, biologically modifying the biofilm living condition to pH=3 would be a proper and achievable aim. For pH=1, it is too hard for any genetically modified bacteria to achieve; and the effect of pH=1 and pH=3 are similar with regard to the degradation level of the biofilm.
Gene Extraction

Bacterial gene extraction: we use the standard protocol included in the extraction kit.
Tissue gene extraction: we used the standard protocol included in the extraction kit.

![](https://static.igem.org/mediawiki/2018/9/9a/T--SHPH-Shanghai--8scr.png)

![](https://static.igem.org/mediawiki/2018/6/62/T--SHPH-Shanghai--chuan.png)
PCR

(1)PCR of S-layer protein

![](https://static.igem.org/mediawiki/2018/3/35/T--SHPH-Shanghai--1biao.png)

![](https://static.igem.org/mediawiki/2018/f/fe/T--SHPH-Shanghai--jpgSlayer.jpg)

(2)PCR of NAG

![](https://static.igem.org/mediawiki/2018/0/0f/T--SHPH-Shanghai--2biao.png)

![](https://static.igem.org/mediawiki/2018/e/e3/T--SHPH-Shanghai--jpg_NAG.jpg)

(3)Fusion PCR

![](https://static.igem.org/mediawiki/2018/c/c0/T--SHPH-Shanghai--3biao.png)