iGEM 2009 — Team Rice

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# Team:Rice/Notebook

### From 2009.igem.org

Eventually this will get formatted differently (better), but until then...

New practices for this year:

In order to better preserve our 2009 parts library, whenever you use a new BioBrick, remove the 15uL of water that you resuspended with, transfer it to a PCR tube, and store in the iGEM 2009 box at -20. Also make sure to list whatever parts you pull on this notebook page. (And, of course, make glycerol stocks of **every** part that you pull from the registry.

5/23/09

Thomas

Pulled parts:

(Tail switching)

BBa\_I719005 - T7 promoter

BBa\_I719015 - T7 promoter test circuit with GFP

BBa\_B0034 - Strong RBS

BBa\_B0015 - commonly used strong forward terminator

BBa\_B1006 - new, strong forward terminator

(Tet selection)

pSB4K5 - low copy Kan vector

(Part for Dr. Beason)

BBa\_J45200

from the 2009 library plates.

Used 1uL of each to HS transform into XL1 competent cells.

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