iGEM 2024 — Team fhs-nb-zj-china

{% extends "layout.html" %}
{% block title %}Notebook{% endblock %}
{% block lead %}{% endblock %}
{% block page\_content %}

## Moments recorded by Yizhou Wang & Lanlan Shan

---

#### March

Our iGEM project started in March 2023. The first important thing was to tidy up a room! As this was the first time our school had participated in such a competition, everything started from scratch: we swept the floor, installed a set of computers, bought new machines and so on.

#### April

After the kick-off meetings, we shared our enthusiasm, got to know each other and officially divided our team into 3 groups: Wiki, Experiment, Publicity. And we set our goal: to use genetic engineering to find a way to cure or alleviate peach tree gummosis disease. To achieve our goal, we need to start with the most basic thing-sterile culture. Almost everyone tried it at least once. At the same time, our wiki was structuring.

#### May

It was May. We added a new group: 3D Printing. They wanted to print a simple fluorometer that would help our work. Our wiki had made great progress! And the sterile plants grew green, and we took care of them. Most of us tried to do DNA extraction from different plants and PCR by using peach gummosis-related SNP primers.

#### June

With the final exams coming up, our work has slowed down, but the team members have been meeting a lot and discussing a lot of things: from solutions to experiments, to wiki programming, to our school life and homework. After the final exams, everything will start again with a vengeance!

#### July

We ran two science programmes for primary and secondary school students, while three groups went to the Ningbo campus of Zhejiang University to learn experimental skills related to genetic engineering.

#### August

We produced our promotional video and had our personal photos taken.

#### September

We now had a -80℃ fridge and an ice machine. So we bought some DH5 alpha competent cells to do the transformation! produced our promotional video and had our personal photos taken.

#### October

Getting ready for the judging section.

{% endblock %}

{% extends "layout.html" %}
{% block title %}Engineering Success{% endblock %}
{% block lead %}{% endblock %}
{% block page\_content %}

## Engineering Success

#### Development of A Simple Fluorometer

#### Instructor: Yunhe Gong; 3D printing: Yuxuan Chen; Co-Authors: Yuxuan Chen, Ziyi Hu, Lanlan Shan

We have developed a simple fluorescence meter with a function similar to that of a qPCR instrument, which is to record the change in fluorescence value in real time, or after the accumulation of a large amount of data, it does not reflect the real-time data recording, but displays some information when it reaches a certain empirical value. By 1st Oct 2024, we have only finished the hardware part.

## Overview

#### A Brief Introduction to the Device Structure

---

The following picture is the casing of our 3D printed fluorometer, which gives a raw green fluorescence value.

![](https://static.igem.wiki/teams/5274/docs/image2.jpeg)

In order to reduce the influence of background light, the entire device is enclosed.

Internally it contains:

1. A light detection module with an LED light source (excitation wavelength is 470 nm, and emission wavelength is 525 nm)

2. A test panel for programming with a LED screen

3. Interchangeable tube modules

4. Power Module

This device is mainly used to make internal and external connections, to limit the position of the light sources, to replace different modules, to fix the touch screen and the main control board.

![](https://static.igem.wiki/teams/5274/docs/image1.jpg)

#### #1 Design

---

The first version addressed the above issues by using Creo software to perform basic design on the rectangular main design through operations such as material removal and stretching:

1. Connect the main control board to the equipment with self tapping screws.

2. Connect the power supply through the side opening.

3. Install a display screen at the top.

4. A groove extends from the side for placing the detection tube.

5. Additionally, design small modules of the same size on the outside and different diameters on the inside to accommodate different detection tubes.

6. The top of the extension slot is designed as a flip cover for easy replacement of modules and detection tubes.

#### #1 Build

---

Please see the [Simple Fluorometer V1](https://static.igem.wiki/teams/5274/photos/v1-2.png) to check out.

#### #1 Test

---

After actual testing, some problems were found:

1. The power hole is not aligned, resulting in the inability to power on.

2. The lid cannot be stably connected to the extension slot.

3. The power limit is too small, causing the limit slot to deform after the power is inserted.

4. There is a significant error in the detected data.

5. There is a hole data mismatch in the connection of the main control board.

6. The maximum detection tube cannot be fully inserted into the extension slot.

#### #1 Learn

---

We need absorb the errors of the #1 version:

1. Data design errors.

2. Some functions have not been considered for improvement.

3. Failure to consider the data of the selected device resulted in some functions being unable to be implemented.

#### #2 Design

---

Based on #1 Learn, we mainly made relevant modifications to the design for some data errors and functional mismatches:

1. Adjusted the distance between the power hole and the reference plane.

2. The connection part of the flip cover originally used a semi-circular structure. Modify the connection part to a wrap around design with an upward opening to achieve detachable and stable rotation of the lid, making it less likely to fall off.

For the purpose of convenience and material saving, the front limit and height limit have been removed for the power limit, while the side limit has been retained and shortened.

The small module for placing the detection tube was originally designed to place the tube vertically. But due to the limited distance of light emitted by the light source. Therefore, the thickness of the wall between the placement port and the light source was reduced, and the tilted placement of the tube was achieved by establishing a cross-section with the vertical plane as a reference.

5. The distance between the fixing hole of the main control board and the reference plane has been changed.

6. Due to the need to adapt to different pipes and be enclosed as a whole, the detection tube needs to be able to be completely placed in the groove. Appropriately increase the overall height of the equipment.

#### #2 Build

---

Please see the [Simple Fluorometer V2](https://static.igem.wiki/teams/5274/photos/v2-2.png) to check out.

#### #2 Test

---

After actual testing, there is a small problems.The power hole is still not properly aligned. However, the unit can be switched on.

#### #2 Learn

---

We need to make some small modifications to make the unit more perfect.

---

## Download the STL Files for Simple fluorometer V2

- Main body: Place chips and other components and make the interior relatively dark

[Click to download the STL File for the main body](https://2024.igem.wiki/fhs-nb-zj-china/static/stl/igem.stl)

- Top cover: Display screen for installing instruments

[Click to download the STL File for the screen holder](https://2024.igem.wiki/fhs-nb-zj-china/static/stl/igemgai.stl)

- Flip cover: Creating a darker environment while facilitating access to centrifuge tubes

[Click to download the STL File for the flip cover](https://2024.igem.wiki/fhs-nb-zj-china/static/stl/fangai.stl)

- A component for installing centrifuge tubes: serving as a fixing mechanism.

[Click to download the STL File for the 1.5 ml centrifuge tube module](https://2024.igem.wiki/fhs-nb-zj-china/static/stl/mokuaida.stl)

{% endblock %}

{% extends "layout.html" %}
{% block title %}Experiments{% endblock %}
{% block lead %}{% endblock %}
{% block page\_content %}

## Our experimental section can be roughly divided into:

---

#### Part 1 Setting up various plant chassis systemsShow

#### Part 2 A little attempt at plasmid manipulationShow

#### Part 3 A quick attempt at cell-free protein expression systemShow

#### Part 4 Troubleshooting of Precipitation in the Stock Solution of 10x MS MacroelementsShow

{% endblock %}

{% extends "layout.html" %}
{% block title %}Results{% endblock %}
{% block lead %}{% endblock %}
{% block page\_content %}

## The results of our work

---

#### We studied the literature and carried out field research to gather as much information as possible.

#### We have completed the hardware part of a simple fluorometer that can be used to diagnose gummosis disease in peach trees.

#### We have studied many experiment skills.

## Future plans for our project

---

#### We will learn as much related knowledge and bioinformatics skills as possible to search for treatment targets.

#### We will collect more data to complete the software part of our simple fluorometer and complete the whole design.

{% endblock %}