iGEM 2020 — Team Shanghai_HS

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:Shanghai HS/Notebook

非模块化方式使用layui

Entprenuership

![](https://static.igem.org/mediawiki/2020/0/0e/T--Shanghai_HS--logo.jpg)

**PhosFATE**

---

* [Home](https://2020.igem.org/Team%3AShanghai_HS)
* Project

  :   [Description](https://2020.igem.org/Team%3AShanghai_HS/Description)
  :   [Design](https://2020.igem.org/Team%3AShanghai_HS/Design)
  :   [Experiments](https://2020.igem.org/Team%3AShanghai_HS/Experiment)
  :   [Notebook](https://2020.igem.org/Team%3AShanghai_HS/Notebook)
  :   [Result](https://2020.igem.org/Team%3AShanghai_HS/Result)
  :   [Safety](https://2020.igem.org/Team%3AShanghai_HS/Safety)
* [Contribution](https://2020.igem.org/Team%3AShanghai_HS/Contribution)
* [Engineering](https://2020.igem.org/Team%3AShanghai_HS/Engineering)
* [Implementation](https://2020.igem.org/Team%3AShanghai_HS/Implementation)
* Parts

  :   [Basic Parts](https://2020.igem.org/Team%3AShanghai_HS/Basic%20Parts)
  :   [Composite Parts](https://2020.igem.org/Team%3AShanghai_HS/Composite%20Parts)
  :   [Parts Collection](https://2020.igem.org/Team%3AShanghai_HS/Parts%20Collection)
* Human Practice

  :   [Integrated Human Practice](https://2020.igem.org/Team%3AShanghai_HS/Human_Practices)
  :   [Fundraising](https://2020.igem.org/Team%3AShanghai_HS/Fundraising)
  :   [Entrepreneurship](https://2020.igem.org/Team%3AShanghai_HS/Entrepreneurship)
* Team

  :   [Team Member](https://2020.igem.org/Team%3AShanghai_HS/Team)
  :   [Collaborations](https://2020.igem.org/Team%3AShanghai_HS/Collaborations)
  :   [Attributions](https://2020.igem.org/Team%3AShanghai_HS/Attributions)

Notebook

![](https://static.igem.org/mediawiki/2020/6/61/T--Shanghai_HS--notebook01.jpg)

Day1

Online
training to introduce the background of the subject and related knowledge.

Day2

（1）The coach leader introduces himself and introduces the
training content；

（2）ice breaker games；

（3）Candidates for captain election, determine the team name,
logo, slogan, uniform and related surroundings；

Day3

Laboratory
safety and basic operation training, molecular biology, protein and cell biology and other biological knowledge
training;

Understand
the common instruments and equipment in biochemical laboratories,
such as centrifuges, PCR machines, sterilization pots, constant temperature shakers, etc., and practice the use of
common instruments, such as micropipettes, etc.

Day4

Understand
the chemical reagents and drugs needed for experiments, learn the use of
balances, measuring cups, and magnetic stirrers, and practice the preparation and sterilization of culture media

Day5

PCR,
and agarose gel Recycle

Learn
the preparation of the PCR reaction system and the use of the PCR machine, learn the role and function of the relevant reagents in the PCR reaction, understand
the specific steps of the PCR reaction program, and be able to operate independently.

Day6

Enzyme
digestion, connection, transformation

Learn
how to use DNA restriction endonucleases and the principle
of action, understand the knowledge of DNA connection and the principle of E. coli transformation, and understand
the basic concepts and operations of genetic engineering

Day7

Pick
bacteria, PCR identification

Learn
the relevant knowledge and operation of colony
PCR, understand the methods and principles of PCR identification, and learn the principles and related operations
of agarose gel electrophoresis

Day8

Sample
delivery for sequencing

Perform
sequencing analysis on the correct bacteria identified
by PCR, verify the correctness of the DNA sequence, and understand the relevant knowledge and principles of gene
sequencing

Day9-10

Escherichia
coli plasmid extraction

Learn
the relevant knowledge of E. coli plasmid extraction, understand the reagent components
in the plasmid extraction kit and their related functions, and be able to operate proficiently

Day11

(Analysis
of sequencing results), phosphate treatment

According
to the results of second-generation sequencing, select the vector to construct the correct
Escherichia coli and treat it with different concentrations of phosphate; then select the appropriate phosphate
concentration and process for different times to obtain the response conditions of the PhoA gene

Day12

Fluorescence
detection, microplate reader detection

Perform
fluorescence detection and analysis on the obtained E. coli samples, and learn how to use the microplate reader

Day13

Data
processing

Perform
relevant processing of experimental data, learn how to use related software, analyze experimental
results, make preliminary judgments on experimental results, analyze the pros and cons of experimental design,
find problems in the experimental process and propose
optimization measures

Contact Email: phosfate2020@163.com

Wechat: Search “PhosFATE”in WeChat

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:Shanghai HS/Engineering

非模块化方式使用layui

Entprenuership

![](https://static.igem.org/mediawiki/2020/0/0e/T--Shanghai_HS--logo.jpg)

**PhosFATE**

---

* [Home](https://2020.igem.org/Team%3AShanghai_HS)
* Project

  :   [Description](https://2020.igem.org/Team%3AShanghai_HS/Description)
  :   [Design](https://2020.igem.org/Team%3AShanghai_HS/Design)
  :   [Experiments](https://2020.igem.org/Team%3AShanghai_HS/Experiment)
  :   [Notebook](https://2020.igem.org/Team%3AShanghai_HS/Notebook)
  :   [Result](https://2020.igem.org/Team%3AShanghai_HS/Result)
  :   [Safety](https://2020.igem.org/Team%3AShanghai_HS/Safety)
* [Contribution](https://2020.igem.org/Team%3AShanghai_HS/Contribution)
* [Engineering](https://2020.igem.org/Team%3AShanghai_HS/Engineering)
* [Implementation](https://2020.igem.org/Team%3AShanghai_HS/Implementation)
* Parts

  :   [Basic Parts](https://2020.igem.org/Team%3AShanghai_HS/Basic%20Parts)
  :   [Composite Parts](https://2020.igem.org/Team%3AShanghai_HS/Composite%20Parts)
  :   [Parts Collection](https://2020.igem.org/Team%3AShanghai_HS/Parts%20Collection)
* Human Practice

  :   [Integrated Human Practice](https://2020.igem.org/Team%3AShanghai_HS/Human_Practices)
  :   [Fundraising](https://2020.igem.org/Team%3AShanghai_HS/Fundraising)
  :   [Entrepreneurship](https://2020.igem.org/Team%3AShanghai_HS/Entrepreneurship)
* Team

  :   [Team Member](https://2020.igem.org/Team%3AShanghai_HS/Team)
  :   [Collaborations](https://2020.igem.org/Team%3AShanghai_HS/Collaborations)
  :   [Attributions](https://2020.igem.org/Team%3AShanghai_HS/Attributions)

![](https://static.igem.org/mediawiki/2020/3/31/T--Shanghai_HS--engineering01.jpg)
![](https://static.igem.org/mediawiki/2020/9/98/T--Shanghai_HS--engineering02.jpg)
![](https://static.igem.org/mediawiki/2020/a/a2/T--Shanghai_HS--engineering03.jpg)

Contact Email: phosfate2020@163.com

Wechat: Search “PhosFATE”in WeChat

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:Shanghai HS/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:Shanghai HS/Results

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)