iGEM 2021 — Team BIT

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# Team:BIT/Notebook

* [Home](https://2021.igem.org/Team%3ABIT)
* Project

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+ [Description](https://2021.igem.org/Team%3ABIT/Description)
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+ [Experiment](https://2021.igem.org/Team%3ABIT/Experiment)
+ [Hardware](https://2021.igem.org/Team%3ABIT/Hardware)
+ [Software](https://2021.igem.org/Team%3ABIT/Software)
+ [Model](https://2021.igem.org/Team%3ABIT/Model)
+ [Engineering](https://2021.igem.org/Team%3ABIT/Engineering)
+ [Implementation](https://2021.igem.org/Team%3ABIT/Implementation)
+ [Proof\_Of\_Concept](https://2021.igem.org/Team%3ABIT/Proof_Of_Concept)
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* Human Practice

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+ [Human Practice](https://2021.igem.org/Team%3ABIT/Human_Practices)
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+ [Communications](https://2021.igem.org/Team%3ABIT/Communication)

* Judge

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+ [Medal](https://2021.igem.org/Team%3ABIT/Medal)
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* Team

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+ [Members](https://2021.igem.org/Team%3ABIT/Members)
+ [Collaborations](https://2021.igem.org/Team%3ABIT/Collaborations)
+ [Partnership](https://2021.igem.org/Team%3ABIT/Partnership)
+ [Notebook](https://2021.igem.org/Team%3ABIT/Notebook)
+ [Attributions](https://2021.igem.org/Team%3ABIT/Attributions)
+ [Excellence\_in\_Another\_Area](https://2021.igem.org/Team%3ABIT/Cultural_and_Creative_Products)

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![](https://static.igem.org/mediawiki/2021/b/b4/T--BIT--top_logo.png)

[First Week](#firstWeek)

![](https://static.igem.org/mediawiki/2021/a/ab/T--BIT--title1.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[February](#February)

![](https://static.igem.org/mediawiki/2021/9/96/T--BIT--title_left.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[March](#March)

![](https://static.igem.org/mediawiki/2021/f/fb/T--BIT--title_right.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[April-May](#April-May)

![](https://static.igem.org/mediawiki/2021/9/96/T--BIT--title_left.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[June](#June)

![](https://static.igem.org/mediawiki/2021/f/fb/T--BIT--title_right.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[July](#July)

![](https://static.igem.org/mediawiki/2021/9/96/T--BIT--title_left.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[August](#August)

![](https://static.igem.org/mediawiki/2021/f/fb/T--BIT--title_right.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[September](#September)

![](https://static.igem.org/mediawiki/2021/9/96/T--BIT--title_left.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[October](#October)

![](https://static.igem.org/mediawiki/2021/f/fb/T--BIT--title_right.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

## Notebook

## First Week

![](https://static.igem.org/mediawiki/2021/4/45/T--BIT--notebook1.png)

## February

![](https://static.igem.org/mediawiki/2021/b/b4/T--BIT--notebook2.png)

## March

![](https://static.igem.org/mediawiki/2021/c/c3/T--BIT--notebook3.png)

## April-May

![](https://static.igem.org/mediawiki/2021/1/13/T--BIT--notebook4.png)

## June

![](https://static.igem.org/mediawiki/2021/1/11/T--BIT--notebook5.png)

## July

![](https://static.igem.org/mediawiki/2021/9/97/T--BIT--notebook6.png)

## August

![](https://static.igem.org/mediawiki/2021/f/f1/T--BIT--notebook7.png)

## September

![](https://static.igem.org/mediawiki/2021/6/67/T--BIT--notebook8.png)

## October

![](https://static.igem.org/mediawiki/2021/4/48/T--BIT--notebook9.png)

## November

![](https://static.igem.org/mediawiki/2021/d/d1/T--BIT--notebook10.png)

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:BIT/Engineering

* [Home](https://2021.igem.org/Team%3ABIT)
* Project

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+ [Description](https://2021.igem.org/Team%3ABIT/Description)
+ [Bio](https://2021.igem.org/Team%3ABIT/Bio)
+ [Parts](https://2021.igem.org/Team%3ABIT/Parts)
+ [Experiment](https://2021.igem.org/Team%3ABIT/Experiment)
+ [Hardware](https://2021.igem.org/Team%3ABIT/Hardware)
+ [Software](https://2021.igem.org/Team%3ABIT/Software)
+ [Model](https://2021.igem.org/Team%3ABIT/Model)
+ [Engineering](https://2021.igem.org/Team%3ABIT/Engineering)
+ [Implementation](https://2021.igem.org/Team%3ABIT/Implementation)
+ [Proof\_Of\_Concept](https://2021.igem.org/Team%3ABIT/Proof_Of_Concept)
+ [Contribution](https://2021.igem.org/Team%3ABIT/Contribution)

* Human Practice

+ Back To Previous Menu
+ [Human Practice](https://2021.igem.org/Team%3ABIT/Human_Practices)
+ [Silver](https://2021.igem.org/Team%3ABIT/Sliver)
+ [Integrated\_Practice](https://2021.igem.org/Team%3ABIT/IHP)
+ [Education](https://2021.igem.org/Team%3ABIT/Education)
+ [Communications](https://2021.igem.org/Team%3ABIT/Communication)

* Judge

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+ [Medal](https://2021.igem.org/Team%3ABIT/Medal)
+ [Safety](https://2021.igem.org/Team%3ABIT/Safety)

* Team

+ Back To Previous Menu
+ [Members](https://2021.igem.org/Team%3ABIT/Members)
+ [Collaborations](https://2021.igem.org/Team%3ABIT/Collaborations)
+ [Partnership](https://2021.igem.org/Team%3ABIT/Partnership)
+ [Notebook](https://2021.igem.org/Team%3ABIT/Notebook)
+ [Attributions](https://2021.igem.org/Team%3ABIT/Attributions)
+ [Excellence\_in\_Another\_Area](https://2021.igem.org/Team%3ABIT/Cultural_and_Creative_Products)

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![](https://static.igem.org/mediawiki/2021/b/b4/T--BIT--top_logo.png)

[Overview](#B_title1)

![](https://static.igem.org/mediawiki/2021/a/ab/T--BIT--title1.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[Summary of Technical Route](#B_title2)

![](https://static.igem.org/mediawiki/2021/9/96/T--BIT--title_left.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[Model Part 1](#B_title3)

![](https://static.igem.org/mediawiki/2021/f/fb/T--BIT--title_right.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[Biology](#B_title4)

![](https://static.igem.org/mediawiki/2021/9/96/T--BIT--title_left.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[Hardware](#B_title5)

![](https://static.igem.org/mediawiki/2021/f/fb/T--BIT--title_right.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[Model Part 2](#B_title6)

![](https://static.igem.org/mediawiki/2021/9/96/T--BIT--title_left.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[Innovation and advantages](#B_title7)

![](https://static.igem.org/mediawiki/2021/f/fb/T--BIT--title_right.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

[Advantages of our device](#B_title8)

![](https://static.igem.org/mediawiki/2021/9/96/T--BIT--title_left.png)
![](https://static.igem.org/mediawiki/2021/8/89/T--BIT--title_mark.png)

Engineering Success

Overview

Our project developed an ultra-sensitive miRNA detection method which based on loop-mediated isothermal amplification (LAMP) and CRISPR/Cas12a trans-cutting technology. To achieve the integration of technical route and operation of early screening for colorectal cancer in our project and overcome the problems that traditional detection methods is not suitable for large-scale early screening due to the complex process and low tolerance of patients, our project established a platform for colorectal cancer early screening in order to achieve rapid, portable and simple "sample-to-answer" integrated detection of colorectal cancer and provide effective intelligent interactive IVD equipment for large-scale early screening.

After testing, our system can operate easily, complete within 1h and its sensitivity can reach fM level. In addition, our device has the ability to detect different miRNAs, which makes it possible to build a standardized cancer detection platform.

![](https://static.igem.org/mediawiki/2021/6/6b/T--BIT--Engineering-100.png)

Opreation of our device

![](https://static.igem.org/mediawiki/2021/2/21/T--BIT--Engineering-101.png)

Model figure of our device

Summary of Technical Route

![](https://static.igem.org/mediawiki/2021/1/19/T--BIT--Engineering-2.jpg)

Model Part 1

At the beginning of the project, we screened CRC-related miRNAs as our biomarkers in the Model module

![](https://static.igem.org/mediawiki/2021/0/0e/T--BIT--Implementation-102.png)

Biology

We firstly constructed a bioassay with high specificity and sensitivity for colorectal cancer related miRNAs.

![](https://static.igem.org/mediawiki/2021/5/5a/T--BIT--Engineering-103.png)

Hardware

In order to achieve rapid and convenient integrated detection, we integrated the detection process constructed by the biological part into the hardware equipment and fused it into the colorectal cancer detection system.

![](https://static.igem.org/mediawiki/2021/d/db/T--BIT--Engineering-104.png)

Model Part 2

The miRNA content detected by our device can be converted into a comprehensive score through the risk prognosis scoring model constructed by the Model module, which can classify the population at risk of CRC.

![](https://static.igem.org/mediawiki/2021/2/28/T--BIT--Engineering-104.jpg)

Innovation and advantages

Bio:

• We developed a joint detection system with LAMP and CRISPR/Cas12aBy designing a pair of specific stem ring DNA probe, the primer design process of LAMP can be greatly simplified.The DNA probe can connect with the target miRNA to form a double- stem ring DNA template, triggering an efficient LAMP reaction and achieving specific amplification with the help of highly specific ligase.

•LAMP amplification products contain a large number of newly generated miRNA-specific sequences, and crRNA can guide the CRISPR/ Cas12a system to specifically recognize these mirNA-specific sequences and activate the Cas12a side chain cutting activity, thus generating obvious fluorescence signal, and the fluorescence signal intensity is positively correlated with the original miRNA content. Therefore, LAMP + CRISPR system can quantitatively detect target miRNA with high sensitivity and specificity.

•After verifying the feasibility of LAMP+CRISPR joint detection system, we further optimized the reaction system, replaced traditional LAMP reagent with LAMP lyophilized bulb, and proved that the amplification effect comparable to the original system could be achieved by using LAMP lyophilized bulb system, which greatly simplified the operation difficulty of the experiment.

Hardware:

•Integrating the biological detection process with the slip chip. The lyophilized bead reagent is pre-embedded in the slip chip to reduce the complex sampling process and the possibility of false positive and false negative due to the non-standard experimental operation

•Machine driven slip chip is used to reduce the uncertainty caused by manual operation, which is convenient to use.

•By constructing the supporting device, our product achieved a high degree of integration of the detection process and avoided the use of enzyme marker, PCR and other large experimental equipment caused by the trouble and it is conducive to the use of large-scale early screening.

•By constructing software system based on Wechat mini program our product achieved the results of visualization and Bluetooth interaction control and it is conducive to the realization of intelligent human-computer interaction process, suitable for the end user experience.

Advantages of our device

**①Portable and integrative platform of cancer early screening with simple operation**

The device of our project has the characteristics of miniaturization, portability and simple operation, the biometric process can be carried out step by step via a machine-driven slip chip without much user involvement. It bases on the development of software interactive page to give users the corresponding operation tips and simplifies the complicated manual operation process in the detection process by the integrated hardware design. Therefore, the detection platform developed by the project is based on the public and does not require the operation of professional technicians, which provides the possibility of large-scale early screening.

![](https://static.igem.org/mediawiki/2021/f/f5/T--BIT--Implementation-6.png)

**②Practical platform of cancer early screening with high sensitivity**

Our project combines the advantages of CRISPR/Cas12 technology for specific recognition and cutting and the advantages of LAMP technology for the the signal of nucleic acid amplification that CRISPR/Cas12 system is highly specific when applied in nucleic acid detection. LAMP technology can perform exponential amplification of target nucleic acid in a short time and improve detection sensitivity. It is this combination of technologies that enables our project to achieve the high sensitivity detection target of fM level.

![](https://static.igem.org/mediawiki/2021/9/9c/T--BIT--Engineering-8.png)

**③ Standardized platform of cancer early screening with high-throughput**

The project builds a corresponding integrated detection platform for early screening of miRNA biomarkers for colorectal cancer and integrates the detection of multiple miRNAs on a slip chip to achieve high-throughput detection system design. In the future, the project can further expand the ability to detect biomarkers and detect multiple cancers, providing corresponding hardware support strategies for the construction of standardized cancer detection platform.

![](https://static.igem.org/mediawiki/2021/0/04/T--BIT--Engineering-9.png)

**④ Opening platform of cancer early screening with extending information**

With the continuous development of information technology such as 5G, internet of things and cloud services, the detection equipment with smart phones as the detection port and data transmission starting point will have great significance. The visualization of the process and results by using WeChat mini programs on smartphones is greatly suitable for end-user experience.

![](https://static.igem.org/mediawiki/2021/e/eb/T--BIT--Engineering-10.png)

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Creat BY Beijing Institute of Technology

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# Team:BIT/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2019 Nantes](https://2019.igem.org/Team%3ANantes/Experiments)
* [2019 TU Eindhoven](https://2019.igem.org/Team%3ATU_Eindhoven/Experiments)
* [2019 Mingdao](https://2019.igem.org/Team%3AMingdao/Demonstrate)
* [2020 Amsterdam](https://2020.igem.org/Team%3AAmsterdam/Experiments)
* [2020 NCTU Formosa](https://2020.igem.org/Team%3ANCTU_Formosa/Experiments)
* [2020 USAFA](https://2020.igem.org/Team%3AUSAFA/Experiments)

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:BIT/Results

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2019 Newcastle](https://2019.igem.org/Team%3ANewcastle/Results)
* [2019 Munich](https://2019.igem.org/Team%3AMunich/Results)
* [2019 Tec Chihuahua](https://2019.igem.org/Team%3ATec-Chihuahua/Results)
* [2020 Aalto Helsinki](https://2020.igem.org/Team%3AAalto-Helsinki/Results)
* [2020 GreatBay SCIE](https://2020.igem.org/Team%3AGreatBay_SCIE/Results)
* [2020 Queens Canada](https://2020.igem.org/Team%3AQueens_Canada/Results)