iGEM 2013 — Team Rutgers

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# Team:Rutgers/Notebook

### From 2013.igem.org

Rutgers notebook

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* [project](https://2013.igem.org/Team%3ARutgers/Project)
* [people](https://2013.igem.org/Team%3ARutgers/People)
* [modelling](https://2013.igem.org/Team%3ARutgers/Modelling)
* [safety](https://2013.igem.org/Team%3ARutgers/Safety)
* [notebook](https://2013.igem.org/Team%3ARutgers/Notebook)
* [practices](https://2013.igem.org/Team%3ARutgers/Practices)

* 6/01

  ## The Rutgers iGEM team sets out to find a lab.
* 6/28

  ## Lab Found!

  Dr. Sagar Khare in the Center for Integrative Proteomics Research offers us laboratory space and supplies to house the Rutgers iGEM team.
* 7/01

  ## First week of July

  Lab safety training: Competent cell preparation, Plate preparation, Plate and cell negative control testing, Confirmation of plate antibiotics and negative controls , Acquisition of CRE recombinase protein from Dr. Pal Maliga at Waksman Institute of Microbiology.

  Transform the following: mRFP (I13521), GFP (I13522), CFP (I13600), mOrange (E2050), LuxR (C0062), LuxI (C0061), LuxBox (R0063), pSB1C3, CRE (in pET29 vector)
* 7/08

  ## Second week of July

  Start overnights of transformations, Minipreps of genes, Digestions of LuxR and LuxBox, Gel Electrophoresis (failed digestion attempt), Start overnights of transformations, Minipreps of genes, Gel Electrophoresis, Ligation of LuxR and LuxBox, Transformation of L1 ( LuxR+LuxBox)
* 7/19

  ## July 15th-19th

  Start overnight of L1, Miniprep L1, Review sequencing data, Failed ligation attempt, Start overnights of transformations, Minipreps of genes, Digestions of LuxR and LuxBox, Gel Electrophoresis (failed digestion attempt), Start overnights of transformations, Minipreps of genes, Gel Electrophoresis, Ligation of LuxR and LuxBox, Transformation of L1 ( LuxR+LuxBox)
* 7/22

  ## July 22nd-26th

  PCR of mRFP loxp site addition, PCR of GFP loxp site addition, Gel electrophoresis confirmation, Digestion of LuxR and LuxBox
* 7/29

  ## July's last days

  Reconsider CRE recombinase project, Quorum sensing degradation design
* 8/5

  ## August!

  New plasmid design for LuxR, LuxI, qsdA degradation, gfp. Order from genscript
* 9/2

  ## First week of September

  Arrival of genscript plasmids, Transformation for BBa\_K1206000, Miniprep BBa\_K1206000, Sequence verification
* 9/16

  ## Mid-September

  Assay for fluorescence of GFP in project plasmid

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