iGEM 2018 — Team BIT

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:BIT/Notebook

<!DOCTYPE html>

Notebook

# NOTEBOOK

## Stage 1(3.26-6.10)

**Three weeks to prepare for Team setup(3.26-4.15)**

**In** Last selection of members
**Out** We have a meet-up with team ZJUT-CHINA in our school
Click [here](https://2018.igem.org/Team%3ABIT/Collaboration) for more

**Week 1(4.16-4.22)**

**In** iGEM\_BIT was established,then we started discussing about our directions of project for this year. Team members were divided into 3 groups. Then was a 3-week-long brainstorm.

**Week 2(4.23-4.29)**

**In** A simple presentation of Interim work from 3 groups.
**Out** We invited our Primary Instructor, Xuefei Lv, to participate in our discuss meeting.

**Week 3(4.30-5.6)**

**In** A final investigate and survey presentation of from 3 groups. Through Voting, team members selected the project that they think is the best.
**Out** This week,we invited Pro. Deng Yulin together with our PI Xuefei Lv ,with their review and our discussion , we determined the primary direction of our project.Interview of Pro.Deng about our project

**Week 4(5.7-5.13)**

**In** We moved on to the next step. And the hold team were focusing on the project and making deeper investigation and survey.
**Out** We want to know how others thought about our JACOB2.0, so we start an online survey trying to collect results. Results will be integrated and analysed in week 7

**Week 5(5.14-5.20)**

**In** Before going into the laboratory, All team members had several laboratory safety training lessons. And nobody have the right to go into the laboratory if he/she didn’t get permission from teacher Yaxi Zhang, who is responsible for the lab safety. In this week, we discussed about the method that might use in biological experiments. And completed reagent selection and purchase also.
**Out** survey on going still

**Week 6(5.21-5.27)**

**In** We studied Genetic engineering experiments like Plasmid extraction and transformation, and practice our experimental skills
**Out** survey on going still

**Week 7(5.28-6.3)**

**In** Kept studying and doing experiments
Microfluidic chip design started
**Out** We collected results of our survey and have conclusions about it. Click [here](https://2018.igem.org/Team%3ABIT/HP/Silver) for more information

**Week 8(6.4-6.10)**

**In** We did preliminary biology experiments.
We also studied PDMS microfluidic chip making

**Out** We went to the clinical laboratory department of aerospace general hospital in Beijing and learned about the related detection method of biomarkers. Click [here](https://2018.igem.org/Team%3ABIT/HP/Silver) for more

## Stage 2(6.11-9.23)

**Week 9(6.11-6.17)**

**In** We did affinity validation between CKMB protein and aptamer
We did experiments of gene circuit 2 connections (inverse ratio line)
We studied PDMS microfluidic chip making

**Out** We have a meet-up with team SDU-CHINA in our school. Click [here](https://2018.igem.org/Team%3ABIT/Collaboration) for more details.

**Week 10(6.18-6.24)**

**In** We did affinity validation between CKMB protein and aptamer
We did experiments of gene circuit 2 connections (inverse ratio line) three times
We started writing safety form carefully. And we participated in the Interlab study.
We studied PDMS microfluidic chip making

**Out** We conducted interviews of local people in remote areas of Guizhou province. Click [here](https://2018.igem.org/Team%3ABIT/HP/Silve) for more

**Week 11(6.25-7.1)**

**In** We did affinity validation between CKMB protein and aptamer together experiments of gene circuit 2 connections (inverse ratio line)
Kept studying PDMS microfluidic chip making

**Out** We have communication with team ZJUT-China about helping them with their biology work.Click [here](https://2018.igem.org/Team%3ABIT/Collaboration) for more

**Week 12(7.2-7.8)**

**In** We did experiments of competitive binding verification of CKMB protein and aptamer, then did interlab experiments of requirement 1 and got some result.
Finished making first edition of chip.And tested its’ function, unfortunately, this edition can not meet our need.
**Out** In order to make sure our design is we went to XXX company, Click [here](https://2018.igem.org/Team%3ABIT/HP/Silver) for more information.

**Week 13(7.9-7.15)**

**In** We did experiments of competitive binding verification of CKMB protein and aptamer
We finished requirement 1 of Interlab. Converting between absorbance of cells to absorbance of a known concentration of beads.
Microfluidic chip redesign started

**Out** Online communication with XJTLU team.Click [here](https://2018.igem.org/Team%3ABIT/Collaboration) for more

**Week 14(7.16-7.22)**

**In** We did experiments of competitive binding verification of CKMB protein and aptamer
We finished requirement 2 Counting colony-forming units (CFUs) from the sample. We made data processing also.
Modeling survey started
Chip making started

**Out** We have communicated with CCU\_Taiwan through Skype and spent two meaningful hours together. Click [here](https://2018.igem.org/Team%3ABIT/Collaboration) for more information.

**Week 15(7.23-7.29)**

**In** did experiments of competitive binding verification of CKMB protein and aptamer
modeling survey started
We submitted our data for Interlab study.
Finished making second edition of chip.And test result is not very good also.So we start learning to make PMMA microfluidic chips in order to make our chip suitable for the hole project.

**Out** Interview of Pro.Deng about our project. Click [here](https://2018.igem.org/Team%3ABIT/HP/Silver) for more

**Week 16(7.30-8.5)**

**In** did experiments of competitive binding verification of CKMB protein and aptamer
modeling survey keep going,and collect some results
did experiments of gene circuit 1 connections
Kept learning making PMMA microfluidic chips

**Out** We contact a professor in School of Mechanical Engineering in our university. He gives us really great advice about how to design our holding apparatus for optical instruments.

**Week 17(8.6-8.12)**

**In** did experiments of gene circuit 1 connections
We tested response of different concentrations of SAM to the circuit
Kept learning making PMMA microfluidic chips,finfished the third edition of chip

**Out** We communicated about our biology design with team XMU-China.
Click [here](https://2018.igem.org/Team%3ABIT/Collaboration) for more

**Week 18(8.13-8.19)**

**In** We tested response of different concentrations of SAM to the circuit
We tested function of chip.It turned out good result.
Device design started

**Out** we went to BOHUI-Tech to investigate about POCT device and share ideas with them. Click [here](https://2018.igem.org/Team%3ABIT/HP/Silver) for more information

**Week 19(8.20-8.26)**

**In** We tested response of different concentrations of SAM to the circuit,
After 3 weeks test and result shown. We found that this method can not meet one of our ultimate goal, quantitative determination. So we are trying to optimize our current methods again. So we discussed and tried to find methods to solve this problem. After discussing, we gave a primary plan.

**Week 20(8.27-9.3)**

**In** Design experiments and purchase reagents.
Complete and check our safety form, then submitted it.

**Week 21(9.3-9.9)**

**In** Preliminary verify the experimental design (aptamer competition and affinity experiment)
Device simulation diagram making.

**Out** Middle school preaching and teaching. Click [here](https://2018.igem.org/Team%3ABIT/HP/Silver) for more

**Week 22(9.10-9.16)**

**In** Flow Cytometric Analysis of the connection between complementary chains and bacteria
Component purchase of device

**Out** preaching for the freshmen in BIT.Click [here](https://2018.igem.org/Team%3ABIT/HP/Silver) for more

**Week 23(9.17-9.23)**

**In** did experiments: linking beads to aptamers, linking aptamers to complementary chains, competitive experiment of protein sample biomarker with complementary chain for aptamer.
device assembly

**Out** High/middle school preaching and teaching. Click [here](https://2018.igem.org/Team%3ABIT/HP/Silver) for more

## Stage 3(9.24-10.16)

**Week 24(9.24-9.30)**

Repeated last week's experiments
presentation practice
Building and filling our wiki page

**Week 25(10.1-10.7)**

Filling in our story on wiki page.
Completed of the most important deliverable carefully--judging form.
Presentation practice again and again
Poster design and making

**Week 26(10.8-10.16)**

Checking our wiki pages and correct small mistakes or fix the bug
Then wiki freeze!!!


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:BIT/Experiments

![](https://static.igem.org/mediawiki/2018/4/4f/2018_igem_mobile_menu_logo.svg)

![](https://static.igem.org/mediawiki/igem.org/3/3b/Menu_icon.svg)

[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3ABIT)
[HOME](https://2018.igem.org/Team%3ABIT)

TEAM

[Team Members](https://2018.igem.org/Team%3ABIT/Team)
[Collaborations](https://2018.igem.org/Team%3ABIT/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ABIT/Description)
[Design](https://2018.igem.org/Team%3ABIT/Design)
[Experiments](https://2018.igem.org/Team%3ABIT/Experiments)
[Notebook](https://2018.igem.org/Team%3ABIT/Notebook)
[InterLab](https://2018.igem.org/Team%3ABIT/InterLab)
[Model](https://2018.igem.org/Team%3ABIT/Model)
[Results](https://2018.igem.org/Team%3ABIT/Results)
[Demonstrate](https://2018.igem.org/Team%3ABIT/Demonstrate)
[Improve](https://2018.igem.org/Team%3ABIT/Improve)
[Attributions](https://2018.igem.org/Team%3ABIT/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ABIT/Parts)
[Basic Parts](https://2018.igem.org/Team%3ABIT/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ABIT/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ABIT/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ABIT/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ABIT/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ABIT/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ABIT/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ABIT/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ABIT/Hardware)
[Measurement](https://2018.igem.org/Team%3ABIT/Measurement)
[Model](https://2018.igem.org/Team%3ABIT/Model)
[Plant](https://2018.igem.org/Team%3ABIT/Plant)
[Software](https://2018.igem.org/Team%3ABIT/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=BIT)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:BIT/Results

![](https://static.igem.org/mediawiki/2018/4/4f/2018_igem_mobile_menu_logo.svg)

![](https://static.igem.org/mediawiki/igem.org/3/3b/Menu_icon.svg)

[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3ABIT)
[HOME](https://2018.igem.org/Team%3ABIT)

TEAM

[Team Members](https://2018.igem.org/Team%3ABIT/Team)
[Collaborations](https://2018.igem.org/Team%3ABIT/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ABIT/Description)
[Design](https://2018.igem.org/Team%3ABIT/Design)
[Experiments](https://2018.igem.org/Team%3ABIT/Experiments)
[Notebook](https://2018.igem.org/Team%3ABIT/Notebook)
[InterLab](https://2018.igem.org/Team%3ABIT/InterLab)
[Model](https://2018.igem.org/Team%3ABIT/Model)
[Results](https://2018.igem.org/Team%3ABIT/Results)
[Demonstrate](https://2018.igem.org/Team%3ABIT/Demonstrate)
[Improve](https://2018.igem.org/Team%3ABIT/Improve)
[Attributions](https://2018.igem.org/Team%3ABIT/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ABIT/Parts)
[Basic Parts](https://2018.igem.org/Team%3ABIT/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ABIT/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ABIT/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ABIT/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ABIT/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ABIT/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ABIT/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ABIT/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ABIT/Hardware)
[Measurement](https://2018.igem.org/Team%3ABIT/Measurement)
[Model](https://2018.igem.org/Team%3ABIT/Model)
[Plant](https://2018.igem.org/Team%3ABIT/Plant)
[Software](https://2018.igem.org/Team%3ABIT/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=BIT)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)