iGEM 2020 — Team MRIIRS_FARIDABAD

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:MRIIRS FARIDABAD/Notebook

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:MRIIRS FARIDABAD/Engineering

T--mriirs\_faridabad--logo.jpg
 Team: MRIIRS FARIDABAD - 2020.igem.org

[![](https://static.igem.org/mediawiki/2020/1/1d/T--mriirs_faridabad--team1Un.png)](https://2020.igem.org/T--mriirs_faridabad--logo)

* Project

  [Description](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Description)
  [Proposed Implementation](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Proposed%20Implementation)
* Engineering Success

  [Design Summary](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Design%20Summary)
  [Lab Notebook](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Lab%20Notebook)
  [Experimentation Protocols](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Experimentation%20Protocols)
  [Safety](https://2020.igem.org/MRIIRS%20FARIDABAD/Safety)
* Contribution

  [Parts](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Parts)
  [literature](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Literature)
* Human Practices

  [Collaboration](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Collaboration)
  [Integrated Human practices](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Integrated%20Human%20Practices)
  [Education & Inclusivity](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Education%20%26%20Inclusivity)
  [Science Communication](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Science%20Communication)
* Project Modelling

  [Overview](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Overview)
  [Parameters](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Parameters)
  [Equations & Models](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Equations%20%26%20Models)
  [Results & Conclusions](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Results%20%26%20Conclusions)
  [References](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/References)
* Li-Koff Excellence Zone

  [Enterpreneurship](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Enterpreneurship)
  [Li-koff entertainment zone](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Li-koff%20entertainment%20zone)
* Team

  [Team Members](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Team%20Members)
  [Attributions](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Attributions)
  [Sponsors](https://2020.igem.org/Team%3AMRIIRS%20FARIDABAD/Sponsors)

* Project

  [Description](soft.html)
  [Proposed Implementation](hard.htm)
  [Proof of Concept](Parts.html)
* Engineering Success

  [Design Summary](exp.html)
  [Lab Notebook](nobook.htm)
  [Experimentation protocols](result.html)
  [Safety](Safety.html)
* Contribution

  [Parts](collab.html)
  [Literature](HumanPractices.html)
* Human Practices

  [Summary](collab.html)
  [Collaboration](HumanPractices.html)
  [Integrated Human Practices](HumanPractices.html)
  [Partnership & Mentorship](HumanPractices.html)
  [Education & Inclusivity](HumanPractices.html)
  [Science Communication](HumanPractices.html)
* Li-Koff Excellence Area

  [Entrepreneurship](collab.html)
  [Entertainment Zone](HumanPractices.html)
* Team

  [Team Members](collab.html)
  [Attributions](HumanPractices.html)
  [Sponsors](HumanPractices.html)
* Project Modeling

  [Overview](collab.html)
  [Parameters](HumanPractices.html)
  [Equations and Model](HumanPractices.html)
  [Result & Conclusion](HumanPractices.html)
  [References](HumanPractices.html)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:MRIIRS FARIDABAD/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:MRIIRS FARIDABAD/Results

E-COLI

![](https://static.igem.org/mediawiki/2020/1/1d/T--mriirs_faridabad--team1Un.png)
[LI-KOFF](main.html)

* Description

  [Description](desc.html)
  [Software](soft.html)
  [Hardware](hard.htm)
  [Parts](Parts.html)
  [Demonstrate](demon.html)
* Process

  [Experiments](exp.html)
  [Notebook](notebook.htm)
  [Results](result.html)
  [Design](design.html)
  [Safety](Safety.html)
* Engagement

  [Collaborations](collab.html)
  [Human Practices](HumanPractices.html)
  [Public Engagement](public.html)
  [Attributions](attribution.html)
* [Awards](awards.html)
* [Team](Team.html)
* [About](about.html)
* [Parts](https://2019.igem.org/Team%3ANYU_Abu_Dhabi/Parts)
* [HUMAN](human-practices.html)

  [Human Practices](human-practices.html)
  [Public Engagement](public-engagement.html)

# Results

![](https://static.igem.org/mediawiki/2020/6/61/T--mriirs_faridabad--team1az.jpg)

Fig.1. PCR amplification using primers specific to the Ada-AlkB promoter sequence.
Ladder used: 25 bp GeneDireX standard ladder

![](https://static.igem.org/mediawiki/2020/a/a6/T--mriirs_faridabad--team1az1.jpg)

Fig.2. PCR amplification using primers specific to the EGFP reporter gene sequence.
Ladder used: NEB-100bp DNA ladder.

![](https://static.igem.org/mediawiki/2020/4/49/T--mriirs_faridabad--team1az2.jpg)

Fig.3.Colony PCR amplification using forward and reverse primer specific to the Ada-AlkB
promoter sequence and the EGFP gene sequence.

![](https://static.igem.org/mediawiki/2020/9/95/T--mriirs_faridabad--team1az3.jpg)

Fig. 4. Benchling results of General testing biobrick assembly

![](https://static.igem.org/mediawiki/2020/a/a5/T--mriirs_faridabad--team1az4.jpg)

![](https://static.igem.org/mediawiki/2020/2/26/T--mriirs_faridabad--team1az5.jpg)

Fig. 5(a, b) Results of virtual digest