iGEM 2016 — Team Wageningen_UR

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# Team:Wageningen UR/Notebook

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* [HOME](https://2016.igem.org/Team%3AWageningen_UR)
* TEAM
  + [Team](https://2016.igem.org/Team%3AWageningen_UR/Team)
  + [★ Collaborations](https://2016.igem.org/Team%3AWageningen_UR/Collaborations)
* PROJECT
  + [★ Description](https://2016.igem.org/Team%3AWageningen_UR/Description)
  + [★ Design](https://2016.igem.org/Team%3AWageningen_UR/Design)
  + [Experiments](https://2016.igem.org/Team%3AWageningen_UR/Experiments)
  + [★ Proof of Concept](https://2016.igem.org/Team%3AWageningen_UR/Proof)
  + [★ Demonstrate](https://2016.igem.org/Team%3AWageningen_UR/Demonstrate)
  + [Results](https://2016.igem.org/Team%3AWageningen_UR/Results)
  + [Notebook](https://2016.igem.org/Team%3AWageningen_UR/Notebook)
* PARTS
  + [Parts](https://2016.igem.org/Team%3AWageningen_UR/Parts)
  + [★ Basic Parts](https://2016.igem.org/Team%3AWageningen_UR/Basic_Part)
  + [★ Composite Parts](https://2016.igem.org/Team%3AWageningen_UR/Composite_Part)
  + [★ Part Collection](https://2016.igem.org/Team%3AWageningen_UR/Part_Collection)
* [SAFETY](https://2016.igem.org/Team%3AWageningen_UR/Safety)
* [★ ATTRIBUTIONS](https://2016.igem.org/Team%3AWageningen_UR/Attributions)
* HUMAN PRACTICES
  + [Human Practices](https://2016.igem.org/Team%3AWageningen_UR/Human_Practices)
  + [★ Silver](https://2016.igem.org/Team%3AWageningen_UR/HP/Silver)
  + [★ Gold](https://2016.igem.org/Team%3AWageningen_UR/HP/Gold)
  + [★ Integrated Practices](https://2016.igem.org/Team%3AWageningen_UR/Integrated_Practices)
  + [★ Engagement](https://2016.igem.org/Team%3AWageningen_UR/Engagement)
* AWARDS
  + [★ Entrepreneurship](https://2016.igem.org/Team%3AWageningen_UR/Entrepreneurship)
  + [★ Hardware](https://2016.igem.org/Team%3AWageningen_UR/Hardware)
  + [★ Software](https://2016.igem.org/Team%3AWageningen_UR/Software)
  + [★ Measurement](https://2016.igem.org/Team%3AWageningen_UR/Measurement)
  + [★ Model](https://2016.igem.org/Team%3AWageningen_UR/Model)

Wageningen UR iGEM 2016

☰
˟

[* **Overview**](https://2016.igem.org/Team%3AWageningen_UR)
[* Description](https://2016.igem.org/Team%3AWageningen_UR/Description)
[* Results](https://2016.igem.org/Team%3AWageningen_UR/Results)
[* Achievements](https://2016.igem.org/Team%3AWageningen_UR/Achievements)
[* BioBricks](https://2016.igem.org/Team%3AWageningen_UR/Parts)
[* Specificity](https://2016.igem.org/Team%3AWageningen_UR/Description/Specificity)
[* Regulation](https://2016.igem.org/Team%3AWageningen_UR/Description/Regulation)
[* Biocontainment](https://2016.igem.org/Team%3AWageningen_UR/Description/Biocontainment)
[* Model](https://2016.igem.org/Team%3AWageningen_UR/Model)
[* Outreach](https://2016.igem.org/Team%3AWageningen_UR/HP/Gold)
[* Team](https://2016.igem.org/Team%3AWageningen_UR/team/members)
[* Notebook](https://2016.igem.org/Team%3AWageningen_UR/Notebook)

* [Overview](https://2016.igem.org/Team%3AWageningen_UR)
  + [Description](https://2016.igem.org/Team%3AWageningen_UR/Description)
  + [Results](https://2016.igem.org/Team%3AWageningen_UR/Results)
  + [Achievements](https://2016.igem.org/Team%3AWageningen_UR/Achievements)
  + [BioBricks](https://2016.igem.org/Team%3AWageningen_UR/Parts)+ [Applied Design](https://2016.igem.org/Team%3AWageningen_UR/Design)
* [WetLab](https://2016.igem.org/Team%3AWageningen_UR/Description)
  + [Specificity](https://2016.igem.org/Team%3AWageningen_UR/Description/Specificity)
    - [*In vitro* assay](https://2016.igem.org/Team%3AWageningen_UR/Description/Specificity#Assay)
    - [Toxin Engineering](https://2016.igem.org/Team%3AWageningen_UR/Description/Specificity#ToxinEngineering)
    - [*Varroa* Isolates](https://2016.igem.org/Team%3AWageningen_UR/Description/Specificity#Isolates)
  + [Regulation](https://2016.igem.org/Team%3AWageningen_UR/Description/Regulation)
    - [Detecting Mites](https://2016.igem.org/Team%3AWageningen_UR/Description/Regulation#DetectingMites)
    - [Population Dynamics](https://2016.igem.org/Team%3AWageningen_UR/Description/Regulation#QuorumSensing)
    - [Toggle Switch](https://2016.igem.org/Team%3AWageningen_UR/Description/Regulation#Toggleswitch)
    - [*E. coli* survival](https://2016.igem.org/Team%3AWageningen_UR/ecoli_survival)
  + [Biocontainment](https://2016.igem.org/Team%3AWageningen_UR/Description/Biocontainment)
    - [Light Kill Switch](https://2016.igem.org/Team%3AWageningen_UR/Description/Biocontainment#Lightswitch)
    - [Cas9 Kill Switch](https://2016.igem.org/Team%3AWageningen_UR/Description/Biocontainment#SAA)
  + [Collaborations](https://2016.igem.org/Team%3AWageningen_UR/Collaborations)
* [Model](https://2016.igem.org/Team%3AWageningen_UR/Model)
  + [Population Dynamics](https://2016.igem.org/Team%3AWageningen_UR/Model#quorum)
  + [Light Kill Switch](https://2016.igem.org/Team%3AWageningen_UR/Model#light)
  + [*E. coli* survival](https://2016.igem.org/Team%3AWageningen_UR/Model#metabolic)
  + [Beehave](https://2016.igem.org/Team%3AWageningen_UR/Model#beehave)
  + [Toxin Scanner](https://2016.igem.org/Team%3AWageningen_UR/Software)
* [Outreach](https://2016.igem.org/Team%3AWageningen_UR/HP/Gold)
  + [Our Vision](https://2016.igem.org/Team%3AWageningen_UR/HP/Gold#Vision)
  + [Design](https://2016.igem.org/Team%3AWageningen_UR/HP/Gold#Design)
  + [Collaboration](https://2016.igem.org/Team%3AWageningen_UR/HP/Gold#collaboration)
  + [Events](https://2016.igem.org/Team%3AWageningen_UR/HP/Gold#events)
  + [Media](https://2016.igem.org/Team%3AWageningen_UR/HP/Gold#media)
  + [Integrated Practices](https://2016.igem.org/Team%3AWageningen_UR/Integrated_Practices)
* [Team](https://2016.igem.org/Team%3AWageningen_UR/team/members)
  + [Members](https://2016.igem.org/Team%3AWageningen_UR/team/members)
    - [Students](https://2016.igem.org/Team%3AWageningen_UR/team/members#students)
    - [Supervisors](https://2016.igem.org/Team%3AWageningen_UR/team/members#supervisors)
  + [Gallery](https://2016.igem.org/Team%3AWageningen_UR/gallery)
  + [Attributions](https://2016.igem.org/Team%3AWageningen_UR/Attributions)
  + [Contact](https://2016.igem.org/Team%3AWageningen_UR/contact)
* [Safety](https://2016.igem.org/Team%3AWageningen_UR/Safety)
  + [Environment](https://2016.igem.org/Team%3AWageningen_UR/Safety#environment)
  + [Laboratory](https://2016.igem.org/Team%3AWageningen_UR/Safety#laboratory)
* [Notebook](https://2016.igem.org/Team%3AWageningen_UR/Notebook)
  + [Journals](https://2016.igem.org/Team%3AWageningen_UR/Notebook)
  + [Protocols](https://2016.igem.org/Team%3AWageningen_UR/Experiments)

![](https://static.igem.org/mediawiki/2016/0/01/T--Wageningen_UR--guf.gif)

![](https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--gufbase.jpg)

### Find the documentation of our research here.

* [![](https://static.igem.org/mediawiki/2016/d/da/T--Wageningen_UR--engibutton.jpg)

  We wanted to engineer Cry3Aa to become toxic to *Varroa destructor*.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/ProteinEngineering)
* [![](https://static.igem.org/mediawiki/2016/5/55/T--Wageningen_UR--vesibutton.jpg)

  To provide an alternative for tests on live *Varroa* mites, we designed an assay that uses brush border membrane vesicles.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/InVitroAssay)
* [![](https://static.igem.org/mediawiki/2016/a/a3/T--Wageningen_UR--mitebutton.jpg)

  We tried to find our own *Varroa* entomopathogen.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/VarroaIsolates)
* [![](https://static.igem.org/mediawiki/2016/f/f4/T--Wageningen_UR--ribobutton.jpg)

  ### Riboswitches

  To detect the presence of *Varroa* mites in the beehive, we made a B12 and a guanine riboswitch.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/Riboswitch)
* [![](https://static.igem.org/mediawiki/2016/b/b1/T--Wageningen_UR--quorumbutton.jpg)

  ### Population Dynamics

  A quorum sensing system was characterized to regulate toxin production in BeeT.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/QuorumSensing)
* [![](https://static.igem.org/mediawiki/2016/6/61/T--Wageningen_UR--lightbutton.jpg)

  ### Optogenetic Kill Switch

  The light-induced kill switch was one of our biocontainment methods.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/OptogeneticKillSwitch)* [![](https://static.igem.org/mediawiki/2016/a/a3/T--Wageningen_UR--mitebutton.jpg)

    ### Chitinases

    As a back-up, we wanted to use chitinases which are more harmful to *Varroa destructor* than honeybees.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/Chitinases)
  * [![](https://static.igem.org/mediawiki/2016/7/76/T--Wageningen_UR--casbutton.jpg)

    ### Cas9-based Kill Switch

    The Cas9-based kill switch was one of our biocontainment methods.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/Cas9)
  * [![](https://static.igem.org/mediawiki/2016/a/a3/T--Wageningen_UR--mitebutton.jpg)

    ### Toxin Scanner

    We made a software tool for finding new Cry proteins toxic to *Varroa destructor*.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/ToxinScanner)
  * [![](https://static.igem.org/mediawiki/2016/5/53/T--Wageningen_UR--metabutton.jpg)

    ### *E. coli* survival

    Following the results of the Metabolic Model, we needed more data.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/ecoli_survival)
  * [![](https://static.igem.org/mediawiki/2016/9/96/T--Wageningen_UR--togglebutton.jpg)

    ### Toggle Switch

    We designed a toggle switch that could coordinate mites sensing, BT's toxin production and light sensing with fast responsiveness.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/toggleswitch)
  * [![](https://static.igem.org/mediawiki/2016/b/bf/T--Wageningen_UR--outreachbutton.jpg)

    ### Human Outreach

    We implemented our outreach into our research as much as we could. Find the details here!](https://2016.igem.org/Team%3AWageningen_UR/Notebook/Outreach)
  * [![](https://static.igem.org/mediawiki/2016/9/96/T--Wageningen_UR--togglebutton.jpg)

    ### BEEHAVE

    We adapted the BEEHAVE model to include BeeT.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/beehave)
* [![](https://static.igem.org/mediawiki/2016/b/b1/T--Wageningen_UR--quorumbutton.jpg)

  ### Population Model

  Modelling of the population dynamics regulation system..](https://2016.igem.org/Team%3AWageningen_UR/Notebook/popmodel)
* [![](https://static.igem.org/mediawiki/2016/6/61/T--Wageningen_UR--lightbutton.jpg)

  ### Kill Switch Model

  The optogenetic kill switch was modeled with ODEs to understand the system in more detail.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/OptogeneticKillSwitchModel)
* [![](https://static.igem.org/mediawiki/2016/5/53/T--Wageningen_UR--metabutton.jpg)

  ### Metabolic Modelling

  We wanted to use a metabolic model to see the effect of our BeeT distribution method.](https://2016.igem.org/Team%3AWageningen_UR/Notebook/MetabolicModelling)

[![](https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg)](#header)

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