iGEM 2015 — Team Lambert_GA

# Team:Lambert GA/Notebook

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[Notebook](https://2015.igem.org/Team%3ALambert_GA/Notebook)
[Attributions](https://2015.igem.org/Team%3ALambert_GA/Attributions)
[Safety](https://2015.igem.org/Team%3ALambert_GA/Safety)
[Human Practices](https://2015.igem.org/Team%3ALambert_GA/Practices)
[Parts](https://2015.igem.org/Team%3ALambert_GA/Parts)

# Notebook

## Calendar

### March

Atlanta Science Festival

Received foldscope from Stanford Prakash Lab

### April

Organized Chick Fil A Biscuit sale fundraiser

Worked with Next Generation Focus to provide science education enrichment

### May

Trained new members in basic lab procedures

### June

Researched yellow stripe rust/ CRISPR cas9 project idea

Researched PelB tags for relocating chitin deacetylase to the periplasm

Emailed FBI agents for outreach project

Researched expression vectors

### July

Narrowed down focus of research to the PelB tags

Designed constructs using 3a assembly method

Designed constructs using gene synthesis method

Decided to use gene synthesis constructs due to resource restraints

Met with Georgia Tech iGEM team; received assistance for construct design

### August

Completed constructs

Added a fusion protein

Practiced transformation procedures

Practiced digest procedures; fixed errors in procedure

Fixed illegal restriction sites in plasmids

Researched bidirectional and bicistronic constructs

Designed and received constructs from IDT

Resuspended sequences from IDT

Received filters from Rosco

Designed team shirts and jackets

### September

Completed wiki

Completed banner

Submitted parts to parts registry

Designed handouts and flyers for Jamboree

Designed "Lab Hacks" outreach project for high school labs

Completed presentation for Jamporee

Completed poster for Jamboree

## Procedures

[![](https://static.igem.org/mediawiki/2015/9/98/Lambert_roscologo.jpg)](https://www.rosco.com/us/index.cfm)

[![](https://static.igem.org/mediawiki/2015/a/a4/Lambert_lhslogo.jpg)](http://www.forsyth.k12.ga.us/lhs)

 [![](https://static.igem.org/mediawiki/2015/8/83/Lambert_sglogo.jpg)](http://www.snapgene.com)

[![](https://static.igem.org/mediawiki/2015/1/10/Lambert_gtlogo.jpg)](http://www.gatech.edu/)

[![](https://static.igem.org/mediawiki/2015/9/90/Lambert_foldscopelogo.jpg)](http://www.foldscope.com/)

[![](https://static.igem.org/mediawiki/2015/c/cb/Lambert_cfalogo.jpg)](http://www.chick-fil-a.com/)

[![](https://static.igem.org/mediawiki/2015/8/80/Lambert_asflogo.jpg)](http://atlantasciencefestival.org/)

[![](https://static.igem.org/mediawiki/2015/e/e4/Lambert_neblogo.jpg)](https://www.neb.com/)

[![](https://static.igem.org/mediawiki/2015/4/46/Lambert_idtlogo.jpg)](https://www.idtdna.com/site)

[![](https://static.igem.org/mediawiki/2015/8/86/Lambert_nsflogo.jpg)](http://www.nsf.gov/)

[![](https://static.igem.org/mediawiki/2015/e/e4/Lambert_ngflogo.jpg)](http://www.nextgenerationfocus.org/)