iGEM 2011 — Team Caltech

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# Team:Caltech/Notebook

### From 2011.igem.org

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Caltech iGEM 2011

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| [**Home**](/Team%3ACaltech "Team:Caltech")     [**Project**](/Team%3ACaltech/Project "Team:Caltech/Project")      [**Data**](/Team%3ACaltech/Data "Team:Caltech/Data")      [**Parts**](/Team%3ACaltech/Parts "Team:Caltech/Parts")      [**Team**](/Team%3ACaltech/Team "Team:Caltech/Team")      ****Notebook****      [**Biosafety**](/Team%3ACaltech/Biosafety "Team:Caltech/Biosafety")      [**Human Impact**](/Team%3ACaltech/Human_Impact "Team:Caltech/Human Impact")      [**References**](/Team%3ACaltech/References "Team:Caltech/References")      [**Support**](/Team%3ACaltech/Acknowledgments "Team:Caltech/Acknowledgments") | [![](https://static.igem.org/mediawiki/2011/1/1f/Caltech_logo.png)](https://2011.igem.org/Team%3ACaltech)  Notebook [Methods and Protocols](/Team%3ACaltech/Protocols "Team:Caltech/Protocols")  [Week 1](/Team%3ACaltech/Week_1 "Team:Caltech/Week 1"): 6/15-6/17: Lab orientation  [Week 2](/Team%3ACaltech/Week_2 "Team:Caltech/Week 2"): 6/19-6/25: Lab training; sample collection, enrichment cultures, DNA extraction from samples  [Week 3](/Team%3ACaltech/Week_3 "Team:Caltech/Week 3"): 6/26-7/2: Prepare parts by PCR for Gibson assembly of test plasmids of BisdA and BisdB, continue enrichment cultures  [Week 4](/Team%3ACaltech/Week_4 "Team:Caltech/Week 4"): 7/3-7/10: Sequencing and testing of Gibson assembled vectors  [Week 5](/Team%3ACaltech/Week_5 "Team:Caltech/Week 5"): 7/11-7/16: Remake Gibson vectors, begin 16S sequencing, run pulsed field gel to extract degradation genes for fosmid kit  [Week 6](/Team%3ACaltech/Week_6 "Team:Caltech/Week 6"): 7/17-7/23: Continuation of Gibson assembly, 16s sequencing and assembly, and fosmid kit  [Week 7](/Team%3ACaltech/Week_7 "Team:Caltech/Week 7"): 7/24-7/30: p450 testing, plating of EDC's, more assembly  [Week 8](/Team%3ACaltech/Week_8 "Team:Caltech/Week 8"): 7/31-8/6: Titering of fosmid kits, HPLC of p450 degradation, more assembly and EDC plating  [Week 9](/Team%3ACaltech/Week_9 "Team:Caltech/Week 9"): 8/7-8/13 GCMS attempts at p450-degraded BPA, biofilm/crystal violet experiments, assembly of constructs and DDT gene  [Week 10](/Team%3ACaltech/Week_10 "Team:Caltech/Week 10"): 8/14-8/20: Electrospray of p450-degraded BPA; visits to local water treatment plants; assembly of constructs, lacZ, and DDT gene  [Week 11](/Team%3ACaltech/Week_11 "Team:Caltech/Week 11"): 8/21-8/27: Assembly of DDT-degradation gene and p450 into BioBrick submission and pET protein expression vectors  [Week 12](/Team%3ACaltech/Week_12 "Team:Caltech/Week 12"): 8/28-9/3: Biofilm-X-gal experiments, sequencing, HPLC  [Week 13](/Team%3ACaltech/Week_13 "Team:Caltech/Week 13"): 9/4-9/10: Quikchange, Biobrick, sequencing  [Week 14](/Team%3ACaltech/Week_14 "Team:Caltech/Week 14"): 9/12-9/18: Redo Quikchange, Biofilm, Protein Expression, HPLC  [Week 15](/Team%3ACaltech/Week_15 "Team:Caltech/Week 15"): 9/19-9/23: Submit parts to registry: DDT gene, bisdA & bisdB, Wt-F87A |

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