iGEM 2025 — Team jiangnan-china


{% extends "layout.html" %}
{% block title %}Engineering{% endblock %}
{% block lead %}Demonstrate engineering success in a technical aspect of your project by going through at least one iteration of the engineering design cycle.{% endblock %}
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{#

#### Silver Medal Criterion #1

Demonstrate engineering success in a technical aspect of your project by going through at least one iteration of the engineering design cycle.

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Visit the [Medals page](https://competition.igem.org/judging/medals) for more information.

## Engineering Design Cycle

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![Engineering Cycle](https://static.igem.wiki/teams/5607/engineer/circle.webp)

### Design Phase

In the design phase, we conceptualize and plan our synthetic biology solution. This involves:

* Identifying the problem and requirements
* Researching existing solutions and parts
* Creating detailed specifications
* Planning the implementation approach

### Build Phase

In the build phase, we construct our biological system. This includes:

* Assembling genetic parts and circuits
* Transforming cells with our constructs
* Verifying successful construction
* Preparing for testing

### Test Phase

In the test phase, we evaluate our system's performance. This involves:

* Conducting functional assays
* Measuring quantitative outputs
* Analyzing data and results
* Identifying areas for improvement

### Learn Phase

In the learn phase, we analyze results and plan improvements. This includes:

* Analyzing test results and data
* Identifying what worked and what didn't
* Planning design modifications
* Preparing for the next iteration

## Engineering Design Cycle

---

![Engineering Cycle](https://static.igem.wiki/teams/5607/engineer/circle.webp)

### Design Phase

In the design phase, we conceptualize and plan our synthetic biology solution. This involves:

* Identifying the problem and requirements
* Researching existing solutions and parts
* Creating detailed specifications
* Planning the implementation approach

### Build Phase

In the build phase, we construct our biological system. This includes:

* Assembling genetic parts and circuits
* Transforming cells with our constructs
* Verifying successful construction
* Preparing for testing

### Test Phase

In the test phase, we evaluate our system's performance. This involves:

* Conducting functional assays
* Measuring quantitative outputs
* Analyzing data and results
* Identifying areas for improvement

### Learn Phase

In the learn phase, we analyze results and plan improvements. This includes:

* Analyzing test results and data
* Identifying what worked and what didn't
* Planning design modifications
* Preparing for the next iteration

## Tips for Success

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Engineering success can be achieved by documenting your effort to follow the engineering design cycle: Design → Build → Test → Learn

We invite you to think about ways to tackle and solve one or more of your project's problems and use synthetic biology tools and/or experimental techniques to generate expected results.

When you have completed the cycle once, think about and document what changes in design you would make for the next iteration(s) of the cycle.

For example, you can design and build a new Part, measure its performance, document whether it worked or not, and propose how the results would inform the next design or steps (documentation must be on the Part's Pages on the Registry).

Visit the [Engineering pages](https://technology.igem.org/engineering) for additional guidance on engineering success.

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{% extends "layout.html" %}
{% block title %}Experiments{% endblock %}
{% block lead %}Describe the research, experiments, and protocols you used in your project. It is designed to provide sufficient information for other teams to replicate our work.{% endblock %}
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{% set sidebar\_title = 'BIOSHIELD Experiments' %}
{% set sidebar\_items = [
{ 'title': 'March', 'icon': 'https://static.igem.wiki/teams/5607/sidebar/weeklylabwork/3.webp', 'href': '#march' },
{ 'title': 'April', 'icon': 'https://static.igem.wiki/teams/5607/sidebar/weeklylabwork/4.webp', 'href': '#april' },
{ 'title': 'May', 'icon': 'https://static.igem.wiki/teams/5607/sidebar/weeklylabwork/5.webp', 'href': '#may' },
{ 'title': 'June', 'icon': 'https://static.igem.wiki/teams/5607/sidebar/weeklylabwork/6.webp', 'href': '#june' },
{ 'title': 'July', 'icon': 'https://static.igem.wiki/teams/5607/sidebar/weeklylabwork/7.webp', 'href': '#july' },
{ 'title': 'August', 'icon': 'https://static.igem.wiki/teams/5607/sidebar/weeklylabwork/8.webp', 'href': '#august' },
{ 'title': 'September', 'icon': 'https://static.igem.wiki/teams/5607/sidebar/weeklylabwork/9.webp', 'href': '#september' }
] %}
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## What Should this Page Contain?

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Provide comprehensive, step-by-step protocols for all experiments conducted. Include a list of materials, reagents, and equipment used. Specify concentrations, volumes, incubation times, temperatures, and other critical parameters. Document any modifications or optimizations made to standard protocols.

Explain the purpose of each experiment and its relevance to your project goals. Describe the experimental design, including controls and replicates. Provide a clear rationale for the chosen methods and approaches.

Document any troubleshooting steps taken and optimizations made during the experimental process. Share any lessons learned that could benefit future teams.

## Inspirations

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* 2024 Heidelberg
* [2024 Marburg](https://2024.igem.wiki/marburg/experiments)
* [2024 EPFL](https://2024.igem.wiki/epfl/experiments)
* [2023 Estonia-TUIT](https://2023.igem.wiki/estonia-tuit/experiments)

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{% extends "layout.html" %}
{% block title %}Results{% endblock %}
{% block lead %}This serves as a chronological record of your team's progress throughout the season. It documents your daily activities, experiments, discussions, and decisions.{% endblock %}
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{% set sidebar\_title = 'BIOSHIELD — Results' %}
{% set sidebar\_items = [
{ 'title': 'Part 1: Preliminary Expression Exploration and Extraction Condition Optimization of LL-37', 'icon': 'https://static.igem.wiki/teams/5607/sidebar/result/part1.webp', 'href': '#part-1-preliminary-expression-exploration-and-extraction-condition-optimization-of-ll-37', 'subs': [
{ 'title': '1.1 Preliminary Expression', 'href': '#11-preliminary-expression-exploration' },
{ 'title': '1.2 Fluorescence Localization', 'href': '#12-fluorescence-localization-experiment' },
{ 'title': '1.3 Extraction Optimization', 'href': '#13-extraction-condition-optimization' }
]
},
{ 'title': 'Part 2: Gene Knockout, Multi-copy Integration and Promoter Engineering to Optimize LL37 Expression', 'icon': 'https://static.igem.wiki/teams/5607/sidebar/result/part2.webp', 'href': '#part-2-gene-knockout-multi-copy-integration-and-promoter-engineering-to-optimize-ll37-expression', 'subs': [
{ 'title': '2.1 Knockout Results', 'href': '#21-multi-copy-fermentation-results-of-all-gene-knockout-strains' },
{ 'title': '2.2 Sequencing Results', 'href': '#22-gene-sequencing-results-of-gene-deletion-strains-from-semi-rational-screening' },
{ 'title': '2.3 Promoter Engineering', 'href': '#23-promoter-engineering-to-enhance-ll37-expression' }
]
},
{ 'title': 'Part 3: Secretion Pathway Construction', 'icon': 'https://static.igem.wiki/teams/5607/sidebar/result/part3.webp', 'href': '#part-3-secretion-pathway-construction', 'subs': [
{ 'title': '3.1 Secretion exploration', 'href': '#31-secretion-pathway-exploration' }
]
},
{ 'title': 'Part 4: Fermentation Condition Optimization and Product Purification Testing', 'icon': 'https://static.igem.wiki/teams/5607/sidebar/result/part4.webp', 'href': '#part-4-fermentation-condition-optimization-and-product-purification-testing', 'subs': [
{ 'title': '4.1 Fermentation', 'href': '#41-fermentation-optimization-of-chassis-cells' },
{ 'title': '4.2 Purification', 'href': '#42-ll-37-purification' }
]
},
{ 'title': 'Part 5: Variant Prediction', 'icon': 'https://static.igem.wiki/teams/5607/sidebar/result/part5.webp', 'href': '#part-5-variant-prediction', 'subs': [
{ 'title': '5.1 Variant Prediction', 'href': '#51-variant-prediction-for-enhanced-antimicrobial-activity' }
]
}
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#### Results

Present the results of your project and discuss their significance.

#### Results

Present the results of your project and discuss their significance.

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