iGEM 2019 — Team RHIT

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:RHIT/Notebook

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[HOME](https://2019.igem.org/Team%3ARHIT)

TEAM

[Team Members](https://2019.igem.org/Team%3ARHIT/Team)
[Collaborations](https://2019.igem.org/Team%3ARHIT/Collaborations)

PROJECT

[Description](https://2019.igem.org/Team%3ARHIT/Description)
[Design](https://2019.igem.org/Team%3ARHIT/Design)
[Experiments](https://2019.igem.org/Team%3ARHIT/Experiments)
[Notebook](https://2019.igem.org/Team%3ARHIT/Notebook)
[Contribution](https://2019.igem.org/Team%3ARHIT/Contribution)
[Results](https://2019.igem.org/Team%3ARHIT/Results)
[Demonstrate](https://2019.igem.org/Team%3ARHIT/Demonstrate)
[Improve](https://2019.igem.org/Team%3ARHIT/Improve)
[Attributions](https://2019.igem.org/Team%3ARHIT/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3ARHIT/Parts)
[Basic Parts](https://2019.igem.org/Team%3ARHIT/Basic_Part)
[Composite Parts](https://2019.igem.org/Team%3ARHIT/Composite_Part)
[Part Collection](https://2019.igem.org/Team%3ARHIT/Part_Collection)

[SAFETY](https://2019.igem.org/Team%3ARHIT/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3ARHIT/Human_Practices)
[Education & Engagement](https://2019.igem.org/Team%3ARHIT/Public_Engagement)

AWARDS

[Entrepreneurship](https://2019.igem.org/Team%3ARHIT/Entrepreneurship)
[Hardware](https://2019.igem.org/Team%3ARHIT/Hardware)
[Measurement](https://2019.igem.org/Team%3ARHIT/Measurement)
[Model](https://2019.igem.org/Team%3ARHIT/Model)
[Plant](https://2019.igem.org/Team%3ARHIT/Plant)
[Software](https://2019.igem.org/Team%3ARHIT/Software)

[JUDGING FORM ⇗](https://igem.org/2019_Judging_Form?team=RHIT)

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)


[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:RHIT/Experiments

![](https://static.igem.org/mediawiki/2019/3/3c/Igem_mobile_menu_logo.svg)

![](https://static.igem.org/mediawiki/2019/e/ee/2019_team_menu_icon.svg)

[![](https://placehold.it/350x150/ececec/cecece)](https://2019.igem.org/Team%3ARHIT)
[HOME](https://2019.igem.org/Team%3ARHIT)

TEAM

[Team Members](https://2019.igem.org/Team%3ARHIT/Team)
[Collaborations](https://2019.igem.org/Team%3ARHIT/Collaborations)

PROJECT

[Description](https://2019.igem.org/Team%3ARHIT/Description)
[Design](https://2019.igem.org/Team%3ARHIT/Design)
[Experiments](https://2019.igem.org/Team%3ARHIT/Experiments)
[Notebook](https://2019.igem.org/Team%3ARHIT/Notebook)
[Contribution](https://2019.igem.org/Team%3ARHIT/Contribution)
[Results](https://2019.igem.org/Team%3ARHIT/Results)
[Demonstrate](https://2019.igem.org/Team%3ARHIT/Demonstrate)
[Improve](https://2019.igem.org/Team%3ARHIT/Improve)
[Attributions](https://2019.igem.org/Team%3ARHIT/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3ARHIT/Parts)
[Basic Parts](https://2019.igem.org/Team%3ARHIT/Basic_Part)
[Composite Parts](https://2019.igem.org/Team%3ARHIT/Composite_Part)
[Part Collection](https://2019.igem.org/Team%3ARHIT/Part_Collection)

[SAFETY](https://2019.igem.org/Team%3ARHIT/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3ARHIT/Human_Practices)
[Education & Engagement](https://2019.igem.org/Team%3ARHIT/Public_Engagement)

AWARDS

[Entrepreneurship](https://2019.igem.org/Team%3ARHIT/Entrepreneurship)
[Hardware](https://2019.igem.org/Team%3ARHIT/Hardware)
[Measurement](https://2019.igem.org/Team%3ARHIT/Measurement)
[Model](https://2019.igem.org/Team%3ARHIT/Model)
[Plant](https://2019.igem.org/Team%3ARHIT/Plant)
[Software](https://2019.igem.org/Team%3ARHIT/Software)

[JUDGING FORM ⇗](https://igem.org/2019_Judging_Form?team=RHIT)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:RHIT/Results

[

](https://static.igem.org/mediawiki/2019/0/0e/T--RHIT--bg.mp4)

![Logo](https://static.igem.org/mediawiki/2019/c/c8/T--RHIT--teamLogo.png)
[RHIT iGEM Wiki](https://2019.igem.org/Team%3ARHIT)

* [Home](https://2019.igem.org/Team%3ARHIT)
* Project

  [Description](https://2019.igem.org/Team%3ARHIT/Description)
  [Design](https://2019.igem.org/Team%3ARHIT/Design)
  [Experiments](https://2019.igem.org/Team%3ARHIT/experiments)
  [Notebook](https://2019.igem.org/Team%3ARHIT/notebook)
  [Model](https://2019.igem.org/Team%3ARHIT/Model)
  [Results](https://2019.igem.org/Team%3ARHIT/Results)
* [Team Members](https://2019.igem.org/Team%3ARHIT/members)
* [Parts](https://2019.igem.org/Team%3ARHIT/Parts)
* [Safety](https://2019.igem.org/Team%3ARHIT/safety)
* Human Practices

  [Overview](https://2019.igem.org/Team%3ARHIT/Human_Practices)
  [Professional Ideas](https://2019.igem.org/Team%3ARHIT/integrated_human_practice)
  [Collaboration](https://2019.igem.org/Team%3ARHIT/Collaborations)
  [Engagement and Education](https://2019.igem.org/Team%3ARHIT/Public_Engagement)
* [Attributions](https://2019.igem.org/Team%3ARHIT/Attributions)
* [Medal](https://2019.igem.org/Team%3ARHIT/Medal)

<!DOCTYPE html>

# Results

### Metallothionein

![](https://static.igem.org/mediawiki/2019/4/40/T--RHIT--A1chart.png)
![](https://static.igem.org/mediawiki/2019/6/65/T--RHIT--A2chart.png)

MT Data

The culmination of testing for this project was to examine how well both constructs (A1 and A2) could handle the bioremediation of arsenic. The full protocol can be found in the lab notebook, and details on the parts used in the design section of the wiki. Our results show that both A1 and A2 were successful at removing arsenite ions from the aqueous solution, and as the total amount of arsenite continued to move down as time increased, cells were able to survive as well. Small decreases were noticed in the negative control as well, as arsenite is naturally transported in and out of cells through aquaglyceroporins (AQPs). In the shorter term (8 hours), A2, which produces metallothionein constantly due to its constitutive promoter, showed better bioremediation of the arsenite. However, at the longer time scale, the arsenic controlled (through ArsR) A1 ended up proving more effective at removing arsenic.

### SOD Enzyme (Characterization)

![](https://static.igem.org/mediawiki/2019/e/e1/T--RHIT--Characterization.png)

Characterization of BBa\_K847004, the protocol for which can be found in the lab notebook, shows that bacteria containing the SOD gene in this experiment have better survivability against the reactive oxygen species produced by copper, including a larger number of final CFUs, as well as survivability at higher concentrations of copper total.

[![](https://static.igem.org/mediawiki/2019/2/24/T--Purdue--twitter_colored.png)](https://twitter.com/RHITiGEM2019)
[![](https://static.igem.org/mediawiki/2019/b/ba/T--RHIT--RHIT_logo.png)](https://www.rose-hulman.edu/)

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