iGEM 2020 — Team NFLS

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:NFLS/Notebook

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:NFLS/Engineering

[![logo](https://static.igem.org/mediawiki/2020/1/1c/T--NFLS--NFLSigem.jpg)](https://2020.igem.org/Team%3ANFLS)

* [Home](https://2020.igem.org/Team%3ANFLS)
* Project

  + [Description](https://2020.igem.org/Team%3ANFLS/Description)
  + [Background](https://2020.igem.org/Team%3ANFLS/Background)
  + [Design](https://2020.igem.org/Team%3ANFLS/Design)
  + [Hardware](https://2020.igem.org/Team%3ANFLS/Hardware)
  + [Engineering Success](https://2020.igem.org/Team%3ANFLS/Engineering)
  + [Implementation](https://2020.igem.org/Team%3ANFLS/Implementation)
* Experiment

  + [Protocol](https://2020.igem.org/Team%3ANFLS/Protocol)
  + [Proof of concept](https://2020.igem.org/Team%3ANFLS/Proof_Of_Concept)
  + [Contribution](https://2020.igem.org/Team%3ANFLS/Contribution)
* [Parts](https://2020.igem.org/Team%3ANFLS/Parts)
* [Safety](https://2020.igem.org/Team%3ANFLS/Safety)
* [Model](https://2020.igem.org/Team%3ANFLS/Model)
* Team

  + [Team members](https://2020.igem.org/Team%3ANFLS/Members)
  + [Attributions](https://2020.igem.org/Team%3ANFLS/Attributions)
  + [Collaborations](https://2020.igem.org/Team%3ANFLS/Collaborations)
  + [Partnership](https://2020.igem.org/Team%3ANFLS/Partnership)
* Human Practices

  + [Overview](https://2020.igem.org/Team%3ANFLS/Overview)
  + [Integrated Human Practice](https://2020.igem.org/Team%3ANFLS/Human_Practices)
  + [Education](https://2020.igem.org/Team%3ANFLS/Education)
* [Judging](https://2020.igem.org/Team%3ANFLS/Judging)

# Engineering

####

Our team is working on the degradation of cellulose. The inspiration of this study comes from our daily life. As high school students, we are surrounded by papers such as test papers and textbooks. However, through investigation, we find that there is no good solution for waste paper and straw. So we looked at cellulose, which is a core component of these substances, in hopes of finding better treatments.

Before that we had a lot of literature reading, browsing the Jay d. Keasling’s paper[1], we know the extracellular hydrolysis of cellulose completely there are several key obstacles, in the technology of biomass and intracellular cellobiose assimilation is an alternative to biological engineering strategy. In our system, we figured out that the most effective way to degrade cellulose is to degrade cellulose to cellobiose and then to glucose. So we used genetically engineered bacteria to build a highly efficient way to break down cellulose into glucose. Endoglucanase is an enzyme that breaks down cellulose into oligosaccharides. It also works with exoglucanase, which breaks down oligosaccharides into fibrinosaccharides and then breaks down glucose under the action of glucosidase. So we performed some experiments on endoglucanase to obtain reliable data for subsequent analysis, including tests of protein glue, SDS-PAGE, and enzyme efficiency assay. In doing so, we discovered that the problem with the design of the device before was that it could only be expressed inside the cell, and it didn't work outside the cell.

In our design, we hope that the element can be expressed in cells, so the introduction of the engineering thinking, have a purpose for further design, on the basis of the original components, topped with a signal peptide of OmpA, added a middle linker would connect it together to form a new part BBa\_K3513000, could perfectly solve the problem that the original cannot be expressed in cells, and make its application more widely, the applicable scope to expand, forming a new part we registered this year. But because the cause of the outbreak of corona virus we were unable to return to new experiments to validate our new component functionality, and in order to compensate for this, we established the mathematical model, and do a variety of enzymes and disaccharide in and out of the dynamical model of engineering bacteria cells, data import to establish mathematical model, through the experiment to verify the feasibility of the improvement measures, as one of the success of the project can be quantitative measure. The research process of the team will be presented on the official website in the form of web pages to describe and explain the principle and model of system work. The team members will complete the team wiki in the way of division of labor, including the project background, description, design, and so on. By recording, we provide complete and detailed experimental inspiration, ideas and procedures so that others can follow our steps to repeat our experiments step by step, and the registered components can also be used by the next IGEM team.

In a word, our team has formed an engineering cycle to think and solve the cellulose decomposition problem we met through engineering thinking.

#### References

> [1] Parisutham, V., Chandran, S. P., Mukhopadhyay, A., Lee, S. K., & Keasling, J. D. (2017). Intracellular cellobiose metabolism and its applications in lignocellulose-based biorefineries. Bioresource technology, 239, 496-506.

![course_img](https://static.igem.org/mediawiki/2020/thumb/c/c3/T--NFLS--Sponsor01.jpg/240px-T--NFLS--Sponsor01.jpg)
![course_img](https://static.igem.org/mediawiki/2020/c/c9/T--NFLS--Sponsor02.jpg)
![course_img](https://static.igem.org/mediawiki/2020/thumb/6/62/T--NFLS--Sponsor03jpg.jpeg/320px-T--NFLS--Sponsor03jpg.jpeg)
![course_img](https://static.igem.org/mediawiki/2020/c/c3/T--NFLS--Sponsor04.jpg)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:NFLS/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:NFLS/Results

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)