iGEM 2021 — Team BJ101ID

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:BJ101ID/Notebook

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2019 Georgia State](https://2019.igem.org/Team%3AGeorgia_State/Notebook)
* [2019 Newcastle](https://2019.igem.org/Team%3ANewcastle/Notebook)
* [2020 IISER Pune India](https://2020.igem.org/Team%3AIISER-Pune-India/Notebook)
* [2020 Lund](https://2020.igem.org/Team%3ALund/Notebook)
* [2020 NOVA LxPortugal](https://2020.igem.org/Team%3ANOVA_LxPortugal/Notebook)
* [2020 RDFZ China](https://2020.igem.org/Team%3ARDFZ-China/NoteBook)

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:BJ101ID/Engineering

[![Logo](https://static.igem.org/mediawiki/2021/9/9d/T--BJ101ID--logo.png)](https://2021.igem.org/Team%3ABJ101ID/index.html)

* [Team](https://2021.igem.org/Team%3ABJ101ID/index.html)
* Project
  + [Description](https://2021.igem.org/Team%3ABJ101ID/Description)
  + [Inplementation](https://2021.igem.org/Team%3ABJ101ID/Inplementation)
  + [Protocol](https://2021.igem.org/Team%3ABJ101ID/Protocol.html)
  + [Safety](https://2021.igem.org/Team%3ABJ101ID/Safety)
  + [Notebook](https://2021.igem.org/Team%3ABJ101ID/Notebook.html)
  + [Proof\_Of\_Concept](https://2021.igem.org/Team%3ABJ101ID/Proof_Of_Concept)
* [Attribution](https://2021.igem.org/Team%3ABJ101ID/Attributions)
* Human Practice
  + [Human\_Practices](https://2021.igem.org/Team%3ABJ101ID/Human_Practices)
  + [Collaboration](https://2021.igem.org/Team%3ABJ101ID/Collaborations)
  + [Communication](https://2021.igem.org/Team%3ABJ101ID/Communication)
  + [Partnership](https://2021.igem.org/Team%3ABJ101ID/Partnership)
  + [Interview](https://2021.igem.org/Team%3ABJ101ID/Interview.html)
  + [Questionnaire survey](https://2021.igem.org/Team%3ABJ101ID/Questionnaire%20survey.html)
* Part
  + [Engineering Success](https://2021.igem.org/Team%3ABJ101ID/Engineering)
  + [Model](https://2021.igem.org/Team%3ABJ101ID/Model)
  + [Contribution](https://2021.igem.org/Team%3ABJ101ID/Contribution)
  + [Improvement](https://2021.igem.org/Team%3ABJ101ID/Improvement)
* Award
  + [Model](https://2021.igem.org/Team%3ABJ101ID/Model)
  + [Improvement](https://2021.igem.org/Team%3ABJ101ID/Improvement)
  + [Partnership](https://2021.igem.org/Team%3ABJ101ID/Partnership)
  + [Education](https://2021.igem.org/Team%3ABJ101ID/Education)

# Engineering Success

## Enginnering

We constructed three plasmids this year and we proved they could all work successfully.
The aim we constructed these three plasmids is to use E.coli BL21(DE3) as a tiny factory to produce sericin and chitosan. So we considered to put the gene in a pET28a(+) plasmid, which is specifically used for E. coli expression. The construction result is proved by DNA sequencing. Then we changed the IPTG concentration, temperature and cultivating time to find the most proper condition. Because the protein we produced are linked with His-tag, so we use his-antibody to perform western blot to confirm its expression.

Part:BBa\_K3874000

pET-28a(+)-Ser-01

![Blog Details Image](https://static.igem.org/mediawiki/2021/0/06/T--BJ101ID--es1.png)

Western Blot Data prove IPTG 0.5mM in 18℃ and a cultivation time of 24 hours is the most proper conditions here, so we used this set of conditions to conduct our experiment.

![Blog Details Image](https://static.igem.org/mediawiki/2021/3/3e/T--BJ101ID--es2.png)

Part:BBa\_K3874001

pET-28a(+)-Ser-08

![Blog Details Image](https://static.igem.org/mediawiki/2021/c/cd/T--BJ101ID--es3.png)

Western Blot Data prove IPTG 0.5mM in 18℃ and a cultivation time of 20 hours is the most proper condition here, so we use this condition in latter experiment.

![Blog Details Image](https://static.igem.org/mediawiki/2021/a/a4/T--BJ101ID--es4.png)

Part:BBa\_K3874002

pET-28a(+)-Chitosan

![Blog Details Image](https://static.igem.org/mediawiki/2021/f/ff/T--BJ101ID--es5.png)

Western Blot Data prove IPTG 0.5mM in 37℃ and a cultivation time of 4 hours is the most proper condition here, so we use this condition in latter experiment.

![Blog Details Image](https://static.igem.org/mediawiki/2021/b/b5/T--BJ101ID--es6.png)

![](https://static.igem.org/mediawiki/2021/9/9d/T--BJ101ID--logo.png)

* Quick Links
* [Team](https://2021.igem.org/Team%3ABJ101ID/index.html)
* [Engineering Success](https://2021.igem.org/Team%3ABJ101ID/Engineering)
* [Model](https://2021.igem.org/Team%3ABJ101ID/Model)
* [Contribution](https://2021.igem.org/Team%3ABJ101ID/Contribution)
* [Partnership](https://2021.igem.org/Team%3ABJ101ID/Partnership)
* [Collaboration](https://2021.igem.org/Team%3ABJ101ID/Collaborations)
* [Human\_Practices](https://2021.igem.org/Team%3ABJ101ID/Human_Practices)

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:BJ101ID/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2019 Nantes](https://2019.igem.org/Team%3ANantes/Experiments)
* [2019 TU Eindhoven](https://2019.igem.org/Team%3ATU_Eindhoven/Experiments)
* [2019 Mingdao](https://2019.igem.org/Team%3AMingdao/Demonstrate)
* [2020 Amsterdam](https://2020.igem.org/Team%3AAmsterdam/Experiments)
* [2020 NCTU Formosa](https://2020.igem.org/Team%3ANCTU_Formosa/Experiments)
* [2020 USAFA](https://2020.igem.org/Team%3AUSAFA/Experiments)

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:BJ101ID/Results

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2019 Newcastle](https://2019.igem.org/Team%3ANewcastle/Results)
* [2019 Munich](https://2019.igem.org/Team%3AMunich/Results)
* [2019 Tec Chihuahua](https://2019.igem.org/Team%3ATec-Chihuahua/Results)
* [2020 Aalto Helsinki](https://2020.igem.org/Team%3AAalto-Helsinki/Results)
* [2020 GreatBay SCIE](https://2020.igem.org/Team%3AGreatBay_SCIE/Results)
* [2020 Queens Canada](https://2020.igem.org/Team%3AQueens_Canada/Results)