iGEM 2016 — Team USP_UNIFESP-Brazil

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# Team:USP UNIFESP-Brazil/Notebook

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AlgAranha Team USP\_UNIFESP-Brazil

[iGEM 2016](https://2016.igem.org/)

* [Home](https://2016.igem.org/wiki/index.php?title=Team:USP_UNIFESP-Brazil)
* [Team](https://2016.igem.org/Team%3AUSP_UNIFESP-Brazil/Team)
* [Project](https://2016.igem.org/Team%3AUSP_UNIFESP-Brazil/Project)
* [Parts](https://2016.igem.org/Team%3AUSP_UNIFESP-Brazil/Parts)
* [Interlab](https://2016.igem.org/Team%3AUSP_UNIFESP-Brazil/Interlab)
* [Human Practices](https://2016.igem.org/Team%3AUSP_UNIFESP-Brazil/Human_Practices)
* [Awards](https://2016.igem.org/Team%3AUSP_UNIFESP-Brazil/Awards)
* [Attributions](https://2016.igem.org/Team%3AUSP_UNIFESP-Brazil/Attributions)

## Notebook

The lab notebook is the window to our soul. Here we describe all those weeks of experiments, with proper attributions to the wetlab iGEMers! We have separated it in two parts. A (more) organized notebook with the experiments curated by topic and the full lab notebook, with everything we documented from the wet lab!

## Full notebook

Here you can find our full notebook, with troubleshooting, annotations, preparation of reagents, optimization of processes and, of course, experiments. If you are brave enough, you can download right below or (depending on your browser) read the text online! You can also find a compilation of all the protocols we used [right here!](https://static.igem.org/mediawiki/2016/3/30/T--USP_UNIFESP-Brazil--Protocols2.pdf)

**TIP: Use the PDF bookmarks to navigate among different weeks of experiments!**

[Click here to download it](https://static.igem.org/mediawiki/2016/0/00/T--USP_UNIFESP-Brazil--labnotebook_fullnotebook_withoutlastweek.pdf)

This browser does not support PDFs. Please download the PDF to view it: [Download PDF](https://static.igem.org/mediawiki/2016/0/00/T--USP_UNIFESP-Brazil--labnotebook_fullnotebook_withoutlastweek.pdf).

## Divided notebook If you get lost by browsing through our complete notebook (we get lost too!), we are providing our divided version by research fronts that were addressed in our project! [Gathering of expression vector units here!](https://static.igem.org/mediawiki/2016/9/9a/T--USP_UNIFESP-Brazil--Gathering_Expression.pdf) This browser does not support PDFs. Please download the PDF to view it: [Download PDF](https://static.igem.org/mediawiki/2016/9/9a/T--USP_UNIFESP-Brazil--Gathering_Expression.pdf). [Download General sequences assembly here!](https://static.igem.org/mediawiki/2016/3/36/T--USP_UNIFESP-Brazil--General_assembling.pdf) This browser does not support PDFs. Please download the PDF to view it: [Download PDF](https://static.igem.org/mediawiki/2016/3/36/T--USP_UNIFESP-Brazil--General_assembling.pdf). [Curious about USER multimerization? here!](https://static.igem.org/mediawiki/2016/2/2c/T--USP_UNIFESP-Brazil--USER.pdf) This browser does not support PDFs. Please download the PDF to view it: [Download PDF](https://static.igem.org/mediawiki/2016/2/2c/T--USP_UNIFESP-Brazil--USER.pdf). [Wanna view Lysostaphin expression?](https://static.igem.org/mediawiki/2016/5/5a/T--USP_UNIFESP-Brazil--Expression_Lysos.pdf) This browser does not support PDFs. Please download the PDF to view it: [Download PDF](https://static.igem.org/mediawiki/2016/5/5a/T--USP_UNIFESP-Brazil--Expression_Lysos.pdf). [Assembly by restriction enzyme of Lh Masp1 Type2](https://static.igem.org/mediawiki/2016/9/9b/T--USP_UNIFESP-Brazil--Assembly_by_restriction_enzyme.pdf) This browser does not support PDFs. Please download the PDF to view it: [Download PDF](https://static.igem.org/mediawiki/2016/9/9b/T--USP_UNIFESP-Brazil--Assembly_by_restriction_enzyme.pdf).