iGEM 2014 — Team SF_Bay_Area_DIYbio

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# Team:SF Bay Area DIYbio/Notebook

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| | |  | | [Home](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio) | [Team](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/Team) | [Official Team Profile](https://igem.org/Team.cgi?year=2014&team_name=SF_Bay_Area_DIYbio) | [Project](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/Project) | [Parts](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/Parts) | [Notebook](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/Notebook) | [Safety](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/Safety) | [Ethical, Legal, Societal, Environmental Issues](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/ELSEI) | [Attributions](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/Attributions) | [Future Plans](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/Future_Plans) | [![](https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png)](https://2014.igem.org/Main_Page) | | | |
| **Notebook**   **General Protocols**     * [Electra Cloning Protocol](https://www.dna20.com/products/expression-vectors/electra-system#2) * [DNA Handling Protocol](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/DNAhandling) * [Media:Zymo E. coli competent cells transformation protocol](https://static.igem.org/mediawiki/2014/1/1e/Zymo_E._coli_competent_cells_transformation_protocol.pdf) * [Media:D4025i (Zyppy plasmid midiprep protocol)](https://static.igem.org/mediawiki/2014/0/0e/Zyppy_plasmid_midiprep_kit_protocol.pdf) * [Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214](https://static.igem.org/mediawiki/2014/4/4d/Clontech_UTF-8%27en-us%27Quick_Easy_Yeast_Transformation_Mix_Protocol-At-A-Glance_040214.pdf) * [Media:Sequencing\_Analysis\_using\_Serial\_Cloner\_2\_16Oct2014‎](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/MediaSequencingAnalysis)    General Documentation Information   * [Real Vegan Cheese Notebook Documentation Guidelines](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/NotebookDocumentation)    **Experiments**  **June 2014**   * [CR\_062214\_humanKappaCasein\_001**22 June 2014**](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/NoteBookJune1)      **August 2014**   * [CLONE\_082514\_humKappaCasein(Kex+)**25 Aug 2014**](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/AugustCloning) * [Growth Curve Experiment **22 Aug 2014**](https://2014.igem.org/Team%3ASF_Bay_Area_DIYbio/AugustGrowthCurve)      **September 2014**   * [Cloning 7 casein gBlocks, **12 Sep 2014**](https://2014.igem.org/cloning7caesin) * [Troubleshooting failed transformation of P.FAKS.humBeta.S on **12 Sept. 2014**](https://2014.igem.org/troubleshooting12.9) * [Cloning bovine beta casein, Fam20C (Kex + & -), from **27Sep2014**](https://2014.igem.org/CloningBovineBetaCaesinSept) * [Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing **30Sep2014**](https://2014.igem.org/Submitting10CaesinSept)      **October 2014**   * [Repeat Midipreps to select new clones for sequencing for failed sequences from **Oct. 2**](https://2014.igem.org/RepeadMidiprepsOct) * [Cloning human alpha casein S1, **04Oct2014**](https://2014.igem.org/CloningHumanAlphaS1Oct) * [Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing **10Oct2014**](https://2014.igem.org/SubmittingPlasmidSequencingOct) * [Submitting FAM20C (Kex + & Kex -) for sequencing with new internal primers **14Oct2014**](https://2014.igem.org/SubmittingFam20COct) | | |

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