iGEM 2016 — Team HokkaidoU_Japan

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# Team:HokkaidoU Japan/Notebook

Team:HokkaidoU Japan - 2016.igem.org

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# Team:HokkaidoU Japan

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# ![](https://static.igem.org/mediawiki/2016/c/cd/T--HokkaidoU_Japan--self-assembring.png)

# ![](https://static.igem.org/mediawiki/2016/6/60/T--HokkaidoU_Japan--teamname.png)

* [Top](https://2016.igem.org/Team%3AHokkaidoU_Japan)
* Project
  + [Overview](https://2016.igem.org/Team%3AHokkaidoU_Japan/Overview)
    - [Project Description](https://2016.igem.org/Team%3AHokkaidoU_Japan/Overview#Project_Description)
    - [Background](https://2016.igem.org/Team%3AHokkaidoU_Japan/Overview#Background)
    - [Proof of concept](https://2016.igem.org/Team%3AHokkaidoU_Japan/Proof%20)
    - [Modeling](https://2016.igem.org/Team%3AHokkaidoU_Japan/Model)
    - [Achievements](https://2016.igem.org/Team%3AHokkaidoU_Japan/Overview#Achievement)
  + [MULTIMERIZATION](https://2016.igem.org/Team%3AHokkaidoU_Japan/Multimerization)
    - [Overview](https://2016.igem.org/Team%3AHokkaidoU_Japan/Multimerization#Overview)
    - [Methods](https://2016.igem.org/Team%3AHokkaidoU_Japan/Multimerization#Methods)
    - [Results](https://2016.igem.org/Team%3AHokkaidoU_Japan/Results%20#Results)
    - [Conclusion](https://2016.igem.org/Team%3AHokkaidoU_Japan/Multimerization#Conclusion)
  + FUTURE WORK
    - [Circularization](https://2016.igem.org/Team%3AHokkaidoU_Japan/Circularization)
    - [Kill Switch](https://2016.igem.org/Team%3AHokkaidoU_Japan/Kill_switch)
    - [Transport](https://2016.igem.org/Team%3AHokkaidoU_Japan/Transport)
    - [Aggregation](https://2016.igem.org/Team%3AHokkaidoU_Japan/Aggregation)
  + Extra Materials
    - [Parts](https://2016.igem.org/Team%3AHokkaidoU_Japan/Parts)
    - [Notebook](https://2016.igem.org/Team%3AHokkaidoU_Japan/Notebook)
    - [Protocols](https://2016.igem.org/Team%3AHokkaidoU_Japan/Experiments)
    - [Safety](https://2016.igem.org/Team%3AHokkaidoU_Japan/Safety%20)
* [Human practices](https://2016.igem.org/Team%3AHokkaidoU_Japan/Human_Practices)
* [Collaborations](https://2016.igem.org/Team%3AHokkaidoU_Japan/Collaborations)
* Team
  + [Attribution](https://2016.igem.org/Team%3AHokkaidoU_Japan/Attributions)
  + [Member](https://2016.igem.org/Team%3AHokkaidoU_Japan/Member)

![](https://static.igem.org/mediawiki/2016/c/ca/T--HokkaidoU_Japan--icon_top.png)

notebook\_2016

![Notebook](https://static.igem.org/mediawiki/2016/e/e7/T--HokkaidoU_Japan--Notebook.png)

[Protocol](https://2016.igem.org/Team%3AHokkaidoU_Japan/Experiments)

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| | August | | | | | | | | --- | --- | --- | --- | --- | --- | --- | |  | 1 | 2 | 3 | 4 | 5 | 6 | | [7](#0807) | 8 | [9](#0809) | [10](#0810) | [11](#0811) | [12](#0812) | [13](#0813) | | [14](#0814) | [15](#0815) | [16](#0816) | [17](#0817) | [18](#0818) | [19](#0819) | [20](#0820) | | [21](#0821) | [22](#0822) | [23](#0823) | [24](#0824) | [25](#0825) | [26](#0826) | [27](#0827) | | [28](#0828) | [29](#0829) | [30](#0830) | [31](#0831) |  |  |  | | | September | | | | | | | | --- | --- | --- | --- | --- | --- | --- | |  |  |  |  | [1](#0901) | [2](#0902) | [3](#0903) | | 4 | [5](#0905) | [6](#0906) | [7](#0907) | [8](#0908) | [9](#0909) | [10](#0910) | | [11](#0911) | [12](#0912) | [13](#0913) | [14](#0914) | [15](#0915) | [16](#0916) | [17](#0917) | | [18](#0918) | [19](#0919) | [20](#0920) | [21](#0921) | [22](#0922) | [23](#0923) | [24](#0924) | | [25](#0925) | [26](#0926) | [27](#0927) | [28](#0928) | [29](#0929) | [30](#0930) |  | | | Octorber | | | | | | | | --- | --- | --- | --- | --- | --- | --- | |  |  |  |  |  |  | [1](#1001) | | [2](#1002) | [3](#1003) | [4](#1004) | [5](#1005) | [6](#1006) | [7](#1007) | [8](#1008) | | [9](#1009) | [10](#1010) | [11](#1011) | [12](#1012) | [13](#1013) | [14](#1014) | [15](#1015) | | [16](#1016) | [17](#1017) | [18](#1018) | [19](#1019) | 20 | 21 | 22 | | 23 | 24 | 25 | 26 | 27 | 28 | 29 | | 30 | 31 |  |  |  |  |  | |

2016/08/07

Transformation

Researcher: Fujita

Part: BBa\_B0034 on pSB1A2, BBa\_E0040 on pSB1A2
BBa\_R0010 on pSB1C3, BBa\_B0015 on pSB1C3,
BBa\_J04500 on pSB1C3, BBa\_E0020 on pSB1C3, BBa\_E0030 on pSB1C3

Liquid Culture

Researcher: Fujita, Kishida, Kono

Part: BBa\_E0020 on pSB1C3, BBa\_E0030 on pSB1C3, BBa\_E0040 on pSB1A2, BBa\_R0010 on pSB1C3

2016/08/09

Transformation

Researcher: Kono, Mimata, Nishimura

Part: BBa\_B0034 on pSB1A2, BBa\_E0040 on pSB1A2,
BBa\_R0010 on pSB1C3, BBa\_B0015 on pSB1C3, BBa\_J04500 on pSB1C3

Mini-prep

Researcher: Fujita, Kishida, Kono

Part: BBa\_R0010 on pSB1A2, BBa\_E0040 on pSB1A2

Gel Free System

Researcher: Mimata, Shibata, Kono, Nishimura

Part: BBa\_R0010, BBa\_E0040

Ethanol Precipitation

Researcher: Mimata, Shibata, Kono, Nishimura

Part: BBa\_R0010, BBa\_E0040

2016/08/10

Liquid Culture

Researcher: Mimata

Part: BBa\_B0015 on pSB1C3, BBa\_J04500 on pSB1C3, BBa\_R0010 on pSB1C3

Transformation

Researcher: Mimata

Part: BBa\_B0034 on pSB1A2, BBa\_E0040 on pSB1A2

Electrophoresis

Researcher: Kishimoto, Kono, Shibata, Nishimura

Part: BBa\_R0010, BBa\_E0040

Mini-prep

Researcher: Mimata, Kono

Part: BBa\_R0010 on pSB1A2, BBa\_B0015 on pSB1C3, BBa\_J04500 on pSB1C3

Electrophoresis

Researcher: Mimata, Kono, Nishimura

Part: BBa\_R0010 on pSB1A2, BBa\_B0015 on pSB1C3, BBa\_J04500 on pSB1C3

Liquid Culture

Researcher: Kono, Mimata, Nishimura

Part: BBa\_B0034 on pSB1A2, BBa\_E0040 on pSB1C3

2016/08/11

Transformation

Researcher: Nishimura, Kishida

Part: BBa\_B0032 on pSB1C3, BBa\_R0011 on pSB1A2

Mini-prep

Researcher: Nishimura, Kishida

Part: BBa\_E0040 on pSB1A2, BBa\_B0034 on pSB1A2

PEG Precipitation

Researcher: Nishimura, Shibata, Noda

Part: BBa\_B0015 on pSB1C3, BBa\_R0010 on pSB1C3
, BBa\_J04500 on pSB1C3, BBa\_E0040 on pSB1A2, BBa\_B0034 on pSB1A2

Electrophoresis

Researcher: Shibata, Noda

Part: BBa\_B0015 on pSB1C3, BBa\_R0010 on pSB1C3
, BBa\_J04500 on pSB1C3, BBa\_E0040 on pSB1A2, BBa\_B0034 on pSB1A2

Gel Free System

Researcher: Kono, Nishimura

Part: BBa\_B0015, BBa\_R0010, BBa\_J04500,
BBa\_E0040, BBa\_B0034

Electrophoresis

Researcher: Nishimura

Part: BBa\_B0015, BBa\_R0010, BBa\_J04500,
BBa\_E0040, BBa\_B0034

Ethanol Precipitation

Researcher: Kono, Nishimura

Part: BBa\_B0015, BBa\_R0010, BBa\_J04500,
BBa\_E0040, BBa\_B0034

Electrophoresis

Researcher: Kono, Nishimura

Part: BBa\_B0015, BBa\_R0010, BBa\_J04500,
BBa\_E0040, BBa\_B0034

2016/08/12

Transformation

Researcher: Nishimura

Part: BBa\_C0012 on pSB1C3, BBa\_J23101 on BBa\_J61002

Liquid Culture

Researcher: Nishimura

Part: BBa\_B0032 on pSB1C3, BBa\_R0011 on pSB1A2

2016/08/13

Mini-prep

Researcher: Fujita

Part: BBa\_B0032 on pSB1C3

PEG Precipitation

Researcher: Fujita

Part: BBa\_B0032 on pSB1C3

Electrophoresis

Researcher: Fujita, Noda

Part: BBa\_B0032 on pSB1C3

Streaking (Single Colony Isolation)

Researcher: Shibata, Fujita

Part: BBa\_R0011 on pSB1A2

Liquid Culture

Researcher: Shibata, Noda

Part: BBa\_C0012 on pSB1C3, BBa\_B0032 on pSB1C3, BBa\_J23101 on BBa\_J61002

2016/08/14

Mini-prep

Researcher: Nishimura, Kishimoto

Part: BBa\_C0012 on pSB1C3, BBa\_B0032 on pSB1C3,
BBa\_J23101 on BBa\_J61002

PEG Precipitation

Researcher: Nishimura, Kono

Part: BBa\_C0012 on pSB1C3, BBa\_B0032 on pSB1C3,
BBa\_J23101 on BBa\_J61002

Electrophoresis

Researcher: Nishimura, Kono

Part: BBa\_C0012 on pSB1C3, BBa\_B0032 on pSB1C3,
BBa\_J23101 on BBa\_J61002

Transformation

Researcher: Nishimura, Kono, Donen

Part: BBa\_R0011 on pSB1A2

2016/08/15

Gel Free System

Researcher: Fujita, Esashi

Part: BBa\_C0012, BBa\_J23101, BBa\_B0032

Electrophoresis

Researcher: Kishida, Fujita, Donen, Shibata, Esashi

Part: BBa\_C0012, BBa\_J23101, BBa\_B0032

Ethanol Precipitation

Researcher: Fujita, Kishida, Noda

Part: BBa\_C0012, BBa\_J23101, BBa\_B0032

Liquid Culture

Researcher: Shibata

Part: BBa\_R0011 on pSB1A2

2016/08/16

Electrophoresis

Researcher: Nishimura

Part: BBa\_C0012, BBa\_J23101, BBa\_B0032

Mini-prep

Researcher: Nishimura

Part: BBa\_R0011 on pSB1A2

PEG Precipitation

Researcher: Nishimura, Kono

Part: BBa\_R0011 on pSB1A2

Electrophoresis

Researcher: Nishimura

Part: BBa\_R0011 on pSB1A2

PCR (2 STEP)

Researcher: Esashi, Nishimura, Shibata

Part: BBa\_R0011

Electrophoresis

Researcher: Nishimura

Part: BBa\_R0011

2016/08/17

Gel Free System

Researcher: Sugishita, Shibata

Part: BBa\_R0011

Electrophoresis

Researcher: Sugishita, Shibata, Kishida

Part: BBa\_R0011

Gel Free System

Researcher: Sugishita, Kishida, Shibata

Part: BBa\_R0011

PEG Precipitation

Researcher: Sugishita, Kishida, Shibata

Part: BBa\_R0011

Transformation

Researcher: Nishimura

Part: BBa\_I13504 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_R0011

Transformation

Researcher: Shibata

Part: BBa\_I13504 on pSB1C3

2016/08/18

Gel Free System

Researcher: Nishimura

Part: BBa\_R0011

Ethanol Precipitation

Researcher:

Part: BBa\_R0011

Electrophoresis

Researcher: Nishimura

Part: BBa\_R0011

Liquid Culture

Researcher: Nishimura

Part: BBa\_I13504 on pSB1C3

Transformation

Researcher: Kono

Part: BBa\_I13504 on pSB1C3

2016/08/19

Mini-prep

Researcher: Shibata, Kishida

Part: BBa\_I13504 on pSB1C3

PEG Precipitation

Researcher: Shibata

Part: BBa\_I13504 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa\_I13504 on pSB1C3

PCR (2 STEP)

Researcher: Nishimura

Part: BBa\_I13504

Electrophoresis

Researcher: Shibata, Nishimura

Part: BBa\_I13504

PCR (2 STEP)

Researcher: Nishimura, Shibata

Part: BBa\_I13504

Electrophoresis

Researcher: Shibata, Nishimura

Part: BBa\_I13504

2016/08/20

Gel Free System

Researcher: Fujita, Mimata

Part: BBa\_I13504

Ethanol Precipitation

Researcher: Fujita, Mimata

Part: BBa\_I13504

2016/08/21

Electrophoresis

Researcher: Nishimura, Mimata, Kishimoto

Part: BBa\_I13504

Ligation

Researcher: Kishimoto, Nishimura, Mimata

Part: BBa\_I13504 &amp; BBa\_R0011 on pSB1A2

Transformation

Researcher: Kishimoto, Kono, Mimata

Part: BBa\_I13401 on pSB1A2,
BBa\_R0011 - BBa\_I13504 on pSB1A2

2016/08/22

Colony PCR

Researcher: Mimata, Kono, Kishida, Esashi

Part: BBa\_R0011 - BBa\_I13504

Liquid Culture

Researcher: Mimata, Kono, Kishida, Esashi

Part: BBa\_R0011 - BBa\_I13504 on pSB1A2, BBa\_I13401 on pSB1A2

2016/08/23

Mini-prep

Researcher: Mimata, Fujita

Part: BBa\_I13401 on pSB1A2

PEG Precipitation

Researcher: Mimata, Fujita

Part: BBa\_I13401 on pSB1A2,
BBa\_R0011 on pSB1A2

Digestion

Researcher: Mimata, Fujita, Yonezawa

Part: BBa\_R0011 on pSB1A2

Gel Free System

Researcher: Mimata, Fujita, Yonezawa

Part: BBa\_I13504

Ethanol Precipitation

Researcher: Mimata, Fujita, Yonezawa

Part: BBa\_I13504

Electrophoresis

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa\_R0011 on pSB1A2, BBa\_I13504

Ligation

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa\_R0011 on pSB1A2 &amp; BBa\_I13504

Electrophoresis

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa\_R0011 - BBa\_I13504 on pSB1A2

Transformation

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa\_R0011 - BBa\_I13504 on pSB1A2

Electrophoresis

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa\_R0011 - BBa\_I13504 on pSB1A2

2016/08/24

Colony PCR

Researcher: Fujita, Kono

Part: BBa\_R0011 - BBa\_I13504

Electrophoresis

Researcher: Fujita, Kono, Shibata, Mimata, Hirao

Part: BBa\_R0011 - BBa\_I13504

PCR (2 STEP)

Researcher: Kono, Shibata, Fujita

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Fujita, Kono, Hirao

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Colony PCR

Researcher: Kono, Hirao

Part: BBa\_R0011 - BBa\_I13504

Digestion

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

PCR (2 STEP)

Researcher: Fujita

Part: BBa\_K2015000, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Hirao

Part: BBa\_R0011 - I13504

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015000, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Colony PCR

Researcher: Fujita, Mimata

Part: BBa\_R0011 - BBa\_I13504

Gel Extract

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Digestion

Researcher: Mimata, Fujita

Part: BBa\_K2015000, pSB1C3

Electrophoresis

Researcher: Mimata, Fujita

Part: pSB1C3, BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Mimata, Fujita

Part: BBa\_R0011 - I13504

2016/08/25

Digestion

Researcher: Mimata

Part: BBa\_J04500, BBa\_I13401

Gel Extract

Researcher: Mimata

Part: pSB1C3, BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Mimata

Part: BBa\_J04500 on pSB1C3, BBa\_I13401 on pSB1A2

Electrophoresis

Researcher: Noda, Mimata

Part: pSB1C3, BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Mimata, Fujita, Noda

Part: pSB1C3, BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Mimata, Fujita

Part: pSB1C3, BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Fujita

Part: pSB1C3, BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Ligation

Researcher: Fujita

Part: pSB1C3 &amp; BBa\_K2015000

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015000 on pSB1C3

PCR (2 STEP)

Researcher: Nishimura

Part: BBa\_K2015012, BBa\_K2015008

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015012, BBa\_K2015008

Transformation

Researcher: Nishimura, Hirao

Part: BBa\_K2015000 on pSB1C3

Gel Free System

Researcher: Shibata, Hirao

Part: BBa\_I13401

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head)

PCR (2 STEP)

Researcher: Nishimura

Part: BBa\_K2015008

Ethanol Precipitation

Researcher: Nishimura, Fujita

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015008

Gel Free System

Researcher: Fujita, Noda, Nishimura, Hirao

Part: BBa\_I13401

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

2016/08/26

Colony PCR

Researcher: Nishimura

Part: BBa\_K2015000

Ethanol Precipitation

Researcher: Hirao, Nishimura, Mimata

Part: BBa\_I13401

Electrophoresis

Researcher: Hirao, Nishimura, Mimata

Part: BBa\_K2015000

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head)

Digestion

Researcher: Hirao, Mimata, Kono

Part: BBa\_K2015000

Electrophoresis

Researcher: Mimata, Kono

Part: BBa\_K2015000

2016/08/27

Liquid Culture

Researcher: Mimata

Part: BBa\_K2015000 on pSB1C3

PCR (2 STEP)

Researcher: Mimata, Hirao

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Digestion

Researcher: Hirao, Mimata, Noda

Part: BBa\_K2015000

Electrophoresis

Researcher: Mimata, Noda, Hirao, Fujita, Shibata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Ethanol Precipitation

Researcher: Fujita, Mimata, Shibata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Digestion

Researcher: Fujita, Noda, Shibata, Hirao

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Hirao, Shibata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Hirao, Shibata, Noda

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Hirao, Shibata

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Shibata, Noda, Esashi

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Ligation

Researcher: Noda, Esashi, Shibata

Part: BBa\_K2015005 - DNMT1 (head) &amp; DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Noda, Esashi, Shibata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

2016/08/28

Mini-prep

Researcher: Mimata

Part: BBa\_K2015000 on pSB1C3

PEG Precipitation

Researcher: Fujita, Mimata, Hirao

Part: BBa\_K2015000 on pSB1C3

Digestion

Researcher: Mimata, Hirao

Part: BBa\_K2015009

PCR (2 STEP)

Researcher: Mimata, Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Mimata, Hirao

Part: BBa\_K2015009

Electrophoresis

Researcher: Mimata, Nishimura, Hirao

Part: BBa\_K2015009, BBa\_K2015000 on pSB1C3

PCR (2 STEP)

Researcher: Mimata, Nishimura, Hirao

Part: BBa\_K2015008

PCR (3 STEP)

Researcher: Mimata, Nishimura, Hirao

Part: BBa\_K2015008

PCR (3 STEP)

Researcher: Mimata, Nishimura, Hirao

Part: BBa\_K2015000

Electrophoresis

Researcher: Mimata, Hirao

Part: BBa\_K2015005 - DNMT1 (head) &amp; DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008

2016/08/29

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000 on pSB1C3

PCR (STEP DOWN)

Researcher: Nishimura

Part: BBa\_K2015008

Colony PCR

Researcher: Nishimura

Part: BBa\_K2015000/span>

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015008

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000

PCR (2 STEP)

Researcher: Mimata, Kishida, Hirao

Part: BBa\_K2015008

Electrophoresis

Researcher: Mimata, Kishida, Hirao

Part: BBa\_K2015000

Electrophoresis

Researcher: Mimata, Kishida, Kono, Hirao

Part: BBa\_K2015008

PCR (2 STEP)

Researcher: Kishida, Kono

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Kono, Hirao

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Ethanol Precipitation

Researcher: Kishida, Hirao, Kono

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

PCR (2 STEP)

Researcher: Kishida, Noda, Esashi

Part: BBa\_K2015000, DNMT1 (tail) - BBa\_K2015005, BBa\_K2015012, BBa\_K2015008

Ethanol Precipitation

Researcher: Noda, Esashi

Part: BBa\_K2015008

Electrophoresis

Researcher: Noda, Esashi

Part: BBa\_K2015000, DNMT1 (tail) - BBa\_K2015005, BBa\_K2015012, BBa\_K2015008

Ethanol Precipitation

Researcher: Kono, Hirao

Part: BBa\_K2015000, DNMT1 (tail) - BBa\_K2015005, BBa\_K2015012, BBa\_K2015008

2016/08/30

PCR (2 STEP)

Researcher: Mimata

Part: BBa\_K2015008

Digestion

Researcher: Hirao, Kono

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Kono, Hirao

Part: BBa\_K2015005 - DNMT1 (head)

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015008

Electrophoresis

Researcher: Hirao, Kono

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Ligation

Researcher: Kono, Hirao

Part: BBa\_K2015005 - DNMT1 (head) &amp; DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Hirao, Kono, Mimata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Mimata, Kono

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

PCR (2 STEP)

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Electrophoresis

Researcher: Shibata, Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Ethanol Precipitation

Researcher: Kishida, Shibata, Hirao

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Electrophoresis

Researcher: Kishida, Hirao

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Digestion

Researcher: Hirao, Kishida, Mimata

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Kishida, Mimata

Part: BBa\_K2015005 - DNMT1 (head)

Electrophoresis

Researcher: Mimata, Nishimura

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Ligation

Researcher: Hirao, Kishida

Part: BBa\_K2015005 - DNMT1 (head) &amp; DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Hirao, Mimata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

CompET15bent Cells

Researcher: Mimata, Hirao, Noda, Esashi, Kono, Fujita

2016/08/31

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

PCR (2 STEP)

Researcher: Nishimura, Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Digestion

Researcher: Kono, Nishimura, Mimata

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009, pSB1C3

Electrophoresis

Researcher: Nishimura, Noda

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Hirao, Mimata, Kishida

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Electrophoresis

Researcher: Nishimura, Noda

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Nishimura, Mimata, Noda, Kono

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Hirao, Kishida

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009, pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Esashi

Part: BBa\_K2015009 on pSB1C3, pSB1C3

Gel Extract

Researcher: Hirao, Kishida, Kono

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Hirao, Kono

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Transformation

Researcher: Kono

Part: pSB1C3

Digestion

Researcher: Esahsi, Mimata

Part: BBa\_K2015000, BBa\_K2015012, pSB1C3

PCR (2 STEP)

Researcher: Kono

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

PCR (2 STEP)

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Esashi

Part: BBa\_K2015000, BBa\_K2015012, pSB1C3

2016/09/01

Electrophoresis

Researcher: Mimata, Esashi, Kono

Part: BBa\_K2015000, BBa\_K2015012, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, pSB1C3

Ethanol Precipitation

Researcher: Kono, Mimata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Ligation

Researcher: Mimata, Kono, Esashi

Part: BBa\_K2015000 &amp; pSB1C3, BBa\_K2015012 &amp; pSB1C3

Digestion

Researcher: Esashi, Mimata

Part: BBa\_K2015009, pSB1C3

Electrophoresis

Researcher: Mimata, Esashi, Kono

Part: BBa\_K2015009, BBa\_K2015000 on pSB1C3, BBa\_K2015012 on pSB1C3, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, pSB1C3

Ethanol Precipitation

Researcher: Kono, Mimata, Esashi

Part: BBa\_K2015009, pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015009, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, pSB1C3

Transformation

Researcher: Nishimura

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015012 on pSB1C3

Digestion

Researcher: Nishimura, Hirao, Shibata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015012, BBa\_K2015009

PCR (2 STEP)

Researcher: Noda, Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Noda

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Electrophoresis

Researcher: Hirao, Shibata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Shibata, Hirao

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015012, BBa\_K2015009

Ethanol Precipitation

Researcher: Shibata, Hirao

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

PCR (2 STEP)

Researcher: Nishimura, Kishida

Part: BBa\_K2015002, BBa\_K2015001

Colony PCR

Researcher: Kono

Part: pSB1C3

Electrophoresis

Researcher: Noda, Esashi

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, BBa\_K2015002, BBa\_K2015001

Ligation

Researcher: Nishimura, Esashi, Noda

Part: BBa\_K2015009 &amp; pSB1C3

Liquid Culture

Researcher: Kono

Part: pSB1C3

PCR (2 STEP)

Researcher: Nishimura, Noda

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Electrophoresis

Researcher: Kono

Part: pSB1C3

Ethanol Precipitation

Researcher: Noda, Esashi, Nishimura

Part: BBa\_K2015009 &amp; pSB1C3

Gel Extract

Researcher: Nishimura, Hirao

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015009 on pSB1C3

PCR (3 STEP)

Researcher: Noda

Part: BBa\_K2015002, BBa\_K2015001

Transformation

Researcher: Nishimura

Part: BBa\_K2015009 on pSB1C3

Gel Extract

Researcher: Hirao

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

2016/09/02

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Electrophoresis

Researcher: Noda

Part: BBa\_K2015002, BBa\_K2015001

Digestion

Researcher: Nishimura

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

PCR Purification

Researcher: Fujita

Part: BBa\_K2015002, BBa\_K2015001

Electrophoresis

Researcher: Fujita, Nishimura

Part: BBa\_K2015002, BBa\_K2015001, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Nishimura, Fujita

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Fujita, Noda

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Colony PCR

Researcher: Fujita

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015012 on pSB1C3

Electrophoresis

Researcher: Fujita, Nishimura, Noda

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015012 on pSB1C3

Ligation

Researcher: Fujita, Noda

Part: BBa\_K2015005 - DNMT1 (head) &amp; DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Nishimura, Noda

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Mini-prep

Researcher: Fujita, Nishimura

Part: pSB1C3

PEG Precipitation

Researcher: Fujita

Part: pSB1C3

Ethanol Precipitation

Researcher: Nishimura

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Fujita, Nishimura

Part: pSB1C3

Colony PCR

Researcher: Hirao, Fujita

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015012 on pSB1C3

PCR (2 STEP)

Researcher: Fujita, Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Digestion

Researcher: Fujita, Hirao

Part: pSB1C3

Transformation

Researcher: Mimata, Hirao

Part: pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015012 on pSB1C3

Ethanol Precipitation

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Hiraa

Part: BBa\_K2015012 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: pSB1C3, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Mimata, Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Digestion

Researcher: Nishimura

Part: pSB1C3

Electrophoresis

Researcher: Nishimura

Part: pSB1C3

Colony PCR

Researcher: Nishimura, Hirao

Part: BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Shibata, Hirap

Part: BBa\_K2015009 on pSB1C3

2016/09/03

Electrophoresis

Researcher: Shibata, Hirap, Mimata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Gel Extract

Researcher: Shibata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Liquid Culture

Researcher: Fujita

Part: pSB1C3

PCR (2 STEP)

Researcher: Fujita, Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Transformation

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Nishimura, Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

2016/09/05

PCR (2 STEP)

Researcher: Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

PCR (2 STEP)

Researcher: Hirao, Shibata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Liquid Culture

Researcher: Mimata

Part: pSB1C3

Digestion

Researcher: Kishida

Part: pSB1C3, BBa\_K2015011

Electrophoresis

Researcher: Kishida, Shibata

Part: pSB1C3, BBa\_K2015011

Ethanol Precipitation

Researcher: Kishida, Shibata

Part: pSB1C3, BBa\_K2015011

Electrophoresis

Researcher: Kishida, Shibata, Mimata

Part: pSB1C3, BBa\_K2015011

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Ligation

Researcher: Shibata, Kishida, Mimata

Part: pSB1C3 &amp; BBa\_K2015011

PCR (2 STEP)

Researcher: Mimata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Ethanol Precipitation

Researcher: Kishida, Shibata

Part: BBa\_K2015011 on pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa\_K2015011 on pSB1C3

Electrophoresis

Researcher: Kishida, Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

PEG Precipitation

Researcher: Fujita, Mimata, Shibata, Kono

Part: pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Dephosphorylation

Researcher: Shibata, Kono, Mimata

Part: pSB1C3

Digestion

Researcher: Shibata, Kono, Mimata

Part: BBa\_K2015011

2016/09/06

Ethanol Precipitation

Researcher: Mimata

Part: BBa\_K2015011

Electrophoresis

Researcher: Mimata, Kono

Part: BBa\_K2015011, pSB1C3

Electrophoresis

Researcher: Kono

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Mini-prep

Researcher: Kono, Shibata

Part: pSB1C3

Digestion

Researcher: Mimata, Shibata

Part: pSB1C3

PEG Precipitation

Researcher: Kono

Part: pSB1C3

Ethanol Precipitation

Researcher: Mimata

Part: pSB1C3

Dephosphorylation

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Mimata, Shibata, Kono

Part: pSB1C3

Electrophoresis

Researcher: Mimata, Shibata, Kono

Part: pSB1C3

Ligation

Researcher: Shibata, Mimata, Kono

Part: BBa\_K2015011 &amp; pSB1C3

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa\_K2015011 on pSB1C3

Transformation

Researcher: Mimata, Shibata

Part: BBa\_K2015011 on pSB1C3

PCR (2 STEP)

Researcher: Kishida, Hirao

Part: BBa\_K2015008

Electrophoresis

Researcher: Kishida, Hirao

Part: BBa\_K2015008

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Gel Extract

Researcher: Kono, Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Hirao, Mimata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Kishida, Mimata

Part: BBa\_K2015008

PCR (2 STEP)

Researcher: Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Digestion

Researcher: Mimata, Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, pET15b

Ethanol Precipitation

Researcher: Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, pET15b

Electrophoresis

Researcher: Hirao, Mimata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, pET15b

Electrophoresis

Researcher: Shibata, Mimata, Kono

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, pET15b

2016/09/07

Gel Extract

Researcher: Kono, Shibata, Mimata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Shibata, Mimata, Kono

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Colony PCR

Researcher: Shibata, Mimata, Kono

Part: BBa\_K2015011 on pSB1C3

Digestion

Researcher: Mimata, Kono, Shibata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Shibata, Mimata, Kono

Part: BBa\_K2015011

Ethanol Precipitation

Researcher: Mimata, Kono

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Mimata, Kono

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Dephosphorylation

Researcher: Shibata

Part: pET15b

Ligation

Researcher: Shibata

Part: pET15b &amp; BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Colony PCR

Researcher: Shibata

Part: BBa\_K2015011

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015011

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005 on pET15b, pET15b

Digestion

Researcher: Kishida

Part: pSB1C3, BBa\_K2015000, BBa\_K2015012

Ethanol Precipitation

Researcher: Kishida

Part: pSB1C3

Ethanol Precipitation

Researcher: Mimata

Part: pSB1C3, BBa\_K2015000, BBa\_K2015012

Electrophoresis

Researcher: Kishida

Part: pSB1C3

Digestion

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008, BBa\_K2015009, pSB1C3

Colony PCR

Researcher: Mimata

Part: BBa\_K2015011

Digestion

Researcher: Kishida

Part: pSB1C3, BBa\_K2015000, BBa\_K2015012

Ethanol Precipitation

Researcher: Mimata

Part: pSB1C3, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008, BBa\_K2015009

Dephosphorylation

Researcher: Mimata

Part: pSB1C3

Ligation

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008, BBa\_K2015009, pSB1C3

Ethanol Precipitation

Researcher: Mimata

Part: pSB1C3, BBa\_K2015000, BBa\_K2015012

Electrophoresis

Researcher: Kishida

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Kishida

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Kishida, Mimata

Part: BBa\_K2015011

Ligation

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head) &amp; DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008 &amp; pSB1C3, BBa\_K2015009 &amp; pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015000, BBa\_K2015012, pSB1C3

Gel Extract

Researcher: Kishida, Hirao

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Transformation

Researcher: Mimata

Part: pSB1C3

Ligation

Researcher: Hirao

Part: BBa\_K2015005 - DNMT1 (head) &amp; DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008 &amp; pSB1C3, BBa\_K2015009 &amp; pSB1C3

Electrophoresis

Researcher: Mimata, Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

2016/09/08

Gel Extract

Researcher: Mimata, Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, BBa\_K2015009 on pSB1C3

Transformation

Researcher: Hirao

Part: BBa\_K2015009 on pSB1C3

Digestion

Researcher: Mimata, Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Ethanol Precipitation

Researcher: Shibata, Mimata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, pSB1C3

Electrophoresis

Researcher: Shibata, Mimata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, pSB1C3

Digestion

Researcher: Shibata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009, pSB1C3

Ligation

Researcher: Hirao, Mimata, Shibata

Part: BBa\_K2015000 &amp; pSB1C3, BBa\_K2015005 - DNMT1 (head) &amp; DNMT1 (tail) - BBa\_K2015005, BBa\_K2015012 &amp; pSB1C3, BBa\_K2015008 &amp; pSB1C3, BBa\_K2015009 &amp; pSB1C3, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005 &amp; pET15b

Digestion

Researcher: Kishida

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009, pSB1C3, pET15b

Ethanol Precipitation

Researcher: Kishida

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009, pSB1C3

Electrophoresis

Researcher: Kishida, Mimata

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015012 on pSB1C3, BBa\_K2015008 on pSB1C3,
BBa\_K2015009 on pSB1C3, BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005 on pET15b, pSB1C3

Colony PCR

Researcher: Hirao

Part: pSB1C3

Ethanol Precipitation

Researcher: Kishida

Part: pET15b

Ethanol Precipitation

Researcher: Mimata, Hirao

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015012 on pSB1C3, BBa\_K2015008 on pSB1C3,
BBa\_K2015009 on pSB1C3, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005 on pET15b, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Dephosphorylation

Researcher: Kishida, Hirao

Part: pSB1C3, pET15b

Transformation

Researcher: Hirao

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005 on pSB1C3, BBa\_K2015012 on pSB1C3

Ligation

Researcher: Hirao

Part: BBa\_K2015008 &amp; pSB1C3

Electrophoresis

Researcher: Mimata

Part: pSB1C3

Transformation

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005 on pET15b

Digestion

Researcher: Shibata

Part: BBa\_K2015000, BBa\_K2015012, pSB1C3

Colony PCR

Researcher: Mimata

Part: BBa\_K2015009

2016/09/09

Ethanol Precipitation

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009

Transformation

Researcher: Mimata

Part: BBa\_K2015008 on pSB1C3

Ethanol Precipitation

Researcher: Shibata

Part: BBa\_K2015000, BBa\_K2015012, pSB1C3

Digestion

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008, BBa\_K2015009, pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015000, BBa\_K2015012, pSB1C3

Digestion

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Ethanol Precipitation

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Ethanol Precipitation

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008, BBa\_K2015009, pSB1C3

Electrophoresis

Researcher: Mimata

Part: pET15b

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Ligation

Researcher: Shibata

Part: BBa\_K2015012, pSB1C3

Gel Extract

Researcher: Mimata

Part: pET15b

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008, BBa\_K2015009, pSB1C3, pET15b

Dephosphorylation

Researcher: Mimata

Part:pSB1C3, pET15b

Ligation

Researcher: Shibata

Part: BBa\_K2015002 &amp; pSB1C3, BBa\_K2015001 &amp; pSB1C3

Electrophoresis

Researcher: Mimata

Part: pSB1C3, pET15b

Ligation

Researcher: Mimata

Part: BBa\_K2015008 &amp; pSB1C3, BBa\_K2015009 &amp; pSB1C3

PCR (2 STEP)

Researcher: Kishida

Part: BBa\_K2015002, BBa\_K2015001

Electrophoresis

Researcher: Kishida

Part: BBa\_K2015012 on pSB1C3, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3, BBa\_K2015002 on pSB1C3, BBa\_K2015001 on pSB1C3

Colony PCR

Researcher: Mimata

Part: BBa\_K2015012, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, BBa\_K2015009

Liquid Culture

Researcher: Kono

Part: pSB1C3

Colony PCR

Researcher: Kono

Part: BBa\_K2015012

PCR Purification

Researcher: Kishida

Part: BBa\_K2015002, BBa\_K2015001

Transformation

Researcher: Mimata

Part: BBa\_K2015012 on pSB1C3, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Ethanol Precipitation

Researcher: Mimata, Kono

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015012, BBa\_K2015009, BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Kono

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015009

Electrophoresis

Researcher: Kono

Part: BBa\_K2015012

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015008

Ethanol Precipitation

Researcher: Mimata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, pET15b

2016/09/10

Digestion

Researcher: Kono

Part: BBa\_K2015012, BBa\_K2015009, pSB1C3

Ethanol Precipitation

Researcher: Kono

Part: BBa\_K2015012, BBa\_K2015009, pSB1C3

PCR (3 STEP)

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Digestion

Researcher: Shibata

Part: pSB1C3

Ethanol Precipitation

Researcher: Shibata

Part: pSB1C3

PCR Purification

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015012, BBa\_K2015009, pSB1C3, pET15b, BBa\_K2015002, BBa\_K2015001

Mini-prep

Researcher: Shibata

Part: pSB1C3

PEG Precipitation

Researcher: Shibata

Part: pSB1C3

PCR (3 STEP)

Researcher: Mimata, Shibata

Part: BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Ligation

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head) &amp; DNMT1 (tail) - BBa\_K2015005

2016/09/11

Ethanol Precipitation

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Dephosphorylation

Researcher: Mimata

Part: pET15b

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Shibata

Part: pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, pET15b

Gel Extract

Researcher: Mimata

Part: pSB1C3

Ligation

Researcher: Mimata

Part: BBa\_K2015000 &amp; pSB1C3

Gel Extract

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005, pSB1C3

PCR (3 STEP)

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

PCR Purification

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Ethanol Precipitation

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Ethanol Precipitation

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015002, BBa\_K2015001

Transformation

Researcher: Mimata

Part: BBa\_K2015000 on pSB1C3

Digestion

Researcher: Mimata

Part: BBa\_K2015011, pSB1C3

Transformation

Researcher: Mimata

Part: pET15b

Ethanol Precipitation

Researcher: Mimata

Part: BBa\_K2015011, pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015011, pSB1C3

Dephosphorylation

Researcher: Mimata

Part: pSB1C3

2016/09/12

Ligation

Researcher: Mimata

Part: BBa\_K2015011 &amp; pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015011 on pSB1C3

Transformation

Researcher: Mimata

Part: BBa\_K2015011 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: pET15b, pSB1C3

PCR (2 STEP)

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Colony PCR

Researcher: Shibata

Part: BBa\_K2015000

Digestion

Researcher: Nishimura

Part: pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Gel Extract

Researcher: Nishimura, Shibata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Gel Extract

Researcher: Nishimura, Shibata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Nishimura

Part: pSB1C3, BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

2016/09/13

Digestion

Researcher: Nishimura, Mimata

Part: pSB1C3

Liquid Culture

Researcher: Mimata

Part: pSB1C3

Transformation

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Mimata, Nishimura

Part: pSB1C3

Digestion

Researcher: Nishimura, Mimata

Part: pSB1C3

Electrophoresis

Researcher: Mimata, Nishimura

Part: pSB1C3

Digestion

Researcher: Nishimura, Mimata

Part: BBa\_K2015000, BBa\_K2015008, BBa\_K2015009

PCR (2 STEP)

Researcher: Nishimura, Hirao

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Gel Extract

Researcher: Mimata, Hirao, Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Digestion

Researcher: Nishimura

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015011, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Ethanol Precipitation

Researcher: Mimata, Nishimura

Part: BBa\_K2015000, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Nishimura, Kishimoto

Part: BBa\_K2015000, BBa\_K2015008, BBa\_K2015009

PCR (2 STEP)

Researcher: Nishimura

Part: BBa\_K2015002, BBa\_K2015001

Electrophoresis

Researcher: Nishimura, Kishimoto

Part: BBa\_K2015002, BBa\_K2015001

Ligation

Researcher: Nishimura

Part: BBa\_K2015000 & pSB1C3, BBa\_K2015009 & pSB1C3, BBa\_K2015008 & BBa\_K2015012 on pSB1C3

Electrophoresis

Researcher: Kono, Hirao

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015009 on pSB1C3

2016/09/14

Transformation

Researcher: Shibata, Kono

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015009 on pSB1C3

Digestion

Researcher: Kono, Shibata

Part: pSB1C3

Electrophoresis

Researcher: Nishimura, Kishimoto, Kishida, Hirao

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, pSB1C3

Ethanol Precipitation

Researcher: Nishimura, Kishida, Hirao, Kishimoto

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Digestion

Researcher: Nishimura, Hirao, Kishimoto

Part: pET15b

Ethanol Precipitation

Researcher: Nishimura, Kishida, Hirao, Kishimoto

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, pET15b

Transformation

Researcher: Nishimura

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1C3

Digestion

Researcher: Nishimura

Part: DNMT1 (tail) - BBa\_K2015005

PCR (2 STEP)

Researcher: Kishida, Nishimura, Kishimoto

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008, BBa\_K2015009

Ethanol Precipitation

Researcher: Nishimura, Kishida

Part: DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008, BBa\_K2015009

Gel Extract

Researcher: Nishimura, Mimata, Kishida

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008, BBa\_K2015009

Ligation

Researcher: Nishimura

Part: BBa\_K2015005 - DNMT1 (head) & DNMT1 (tail) - BBa\_K2015005

Colony PCR

Researcher: Mimata, Hirao, Shibata

Part: BBa\_K2015009

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015000, BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005, BBa\_K2015008

Electrophoresis

Researcher: Shibata, Hirao, Noda, Kono

Part: BBa\_K2015009

2016/09/15

Liquid Culture

Researcher: Shibata, Noda, Kono

Part: pSB1C3

PCR (2 STEP)

Researcher: Kono, Noda

Part: BBa\_K2015009

Electrophoresis

Researcher: Shibata, Noda, Kono

Part: BBa\_K2015000

Electrophoresis

Researcher: Shibata, Kono

Part: BBa\_K2015009

Gel Extract

Researcher: Shibata, Kono, Noda

Part: BBa\_K2015009

Electrophoresis

Researcher: Shibata, Kono, Noda

Part: BBa\_K2015009

Colony PCR

Researcher: Fujita

Part: BBa\_K2015008

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Gel Extract

Researcher: Fujita

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015005 - DNMT1 (head) - DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015008

Digestion

Researcher: Fujita

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Colony PCR

Researcher: Fujita

Part: BBa\_K2015012 - BBa\_K2015008

Ethanol Precipitation

Researcher: Nishimura

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Electrophoresis

Researcher: Kishimoto, Nishimura

Part: BBa\_K2015005 - DNMT1 (head), DNMT1 (tail) - BBa\_K2015005

Colony PCR

Researcher: Nishimura

Part: BBa\_K2015012 - BBa\_K2015008

Colony PCR

Researcher: Fujita

Part: BBa\_K2015012 - BBa\_K2015008

Mini-prep

Researcher: Nishimura, Kishimoto

Part: BBa\_K2015009 on pSB1C3

Mini-prep

Researcher: Fujita

Part: BBa\_K2015000 on pSB1C3, pSB1C3

PEG Precipitation

Researcher: Fujita, Hirao

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015009 on pSB1C3, pSB1C3

Electrophoresis

Researcher: Fujita, Hirao

Part: BBa\_K2015012 - BBa\_K2015008

Electrophoresis

Researcher: Fujita, Hirao

Part: BBa\_K2015009 on pSB1C3, BBa\_K2015000 on pSB1C3, pSB1C3

PCR (3 STEP)

Researcher: Fujita, Hirao

Part: BBa\_K2015000, BBa\_K2015009

Digestion

Researcher: Fujita, Hirao

Part: pSB1C3, BBa\_K2015011

2016/09/16

Electrophoresis

Researcher: Fujita, Hirao

Part: pSB1C3, BBa\_K2015011

Ethanol Precipitation

Researcher: Mimata, Fujita, Hirao

Part: BBa\_K2015000, BBa\_K2015009

Sequencing

Researcher: Fujita, Hirao, Mimata

Part: BBa\_K2015000, BBa\_K2015009

Ligation

Researcher: Hirao, Mimata, Fujita

Part: BBa\_K2015011 & pSB1C3

Electrophoresis

Researcher: Mimata, Hirao, Kono

Part: BBa\_K2015011 on pSB1C3

Transformation

Researcher: Mimata, Kono

Part: BBa\_K2015011 on pSB1C3

Colony PCR

Researcher: Nishimura

Part: BBa\_K2015012 - BBa\_K2015008

Ligation

Researcher: Kono, Nishimura

Part: BBa\_K2015005 - DNMT1 (head) & DNMT1 (tail) - BBa\_K2015005

PEG Precipitation

Researcher: Kono

Part: BBa\_K2015000 on pSB1C3

Electrophoresis

Researcher: Kono, Fujita, Nishimura

Part: BBa\_K2015000 on pSB1C3

Electrophoresis

Researcher: Kono, Fujita, Nishimura

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3

Sequencing

Researcher: Fujita, Kishida

Part: BBa\_K2015000, BBa\_K2015009

Liquid Culture

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1A3, BBa\_K2015008 on pSB1A3

Ligation

Researcher: Nishimura

Part: BBa\_K2015008 & pSB1C3

Ethanol Precipitation

Researcher: Fujita, Hirao, Shibata

Part: BBa\_K2015000, BBa\_K2015009

Colony PCR

Researcher: Kishida, Fujita, Shibata, Hirao

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015009

Transformation

Researcher: Fujita

Part: BBa\_K2015008 on pSB1C3

2016/09/17

Electrophoresis

Researcher: Fujita, Hirao, Mimata

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015009

Liquid Culture

Researcher: Mimata

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015009 on pSB1C3

Digestion

Researcher: Nishimura

Part: pSB1C3

Mini-prep

Researcher: Nishimura

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

PEG Precipitation

Researcher: Nishimura

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

Electrophoresis

Researcher: Nishimura, Kishida

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_K2015011

Ligation

Researcher: Nishimura

Part: BBa\_K2015011 & pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

Transformation

Researcher: Kishida

Part: BBa\_K2015011 on pSB1C3

Sequencing

Researcher: Shidata, Nishimura

Part: BBa\_K2015000, BBa\_K2015009

Colony PCR

Researcher: Nishimura

Part: BBa\_K2015011

Transformation

Researcher: Nishimura

Part: BBa\_K2015011 on pSB1C3

Colony PCR

Researcher: Nishimura, Shibata

Part: BBa\_K2015008

Digestion

Researcher: Shibata

Part: BBa\_K2015011, pSB1C3

Ethanol Precipitation

Researcher: Shibata, Nishimura

Part: BBa\_K2015011, pSB1C3

Electrophoresis

Researcher: Kono, Nishimura, Shibata

Part: BBa\_K2015011, pSB1C3

Ethanol Precipitation

Researcher: Mimata, Kono

Part: BBa\_K2015000, BBa\_K2015012 - BBa\_K2015008, BBa\_K2015009

Digestion

Researcher: Mimata, Kono

Part: BBa\_K2015011

2016/09/18

Ethanol Precipitation

Researcher: Shibata, Mimata, Kono

Part: BBa\_K2015011

Mini-prep

Researcher: Mimata, Shibata, Kono

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Kono, Mimata, Shibata

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015009 on pSB1C3, BBa\_K2015011

PEG Precipitation

Researcher: Mimata, Shibata, Kono

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Kono, Mimata, Fujita

Part: BBa\_K2015008

Ligation

Researcher: Fujita, Kono, Mimata

Part: BBa\_K2015011 & pSB1C3

Liquid Culture

Researcher: Mimata, Kono

Part: BBa\_K2015008 on pSB1C3

Electrophoresis

Researcher: Kono, Mimata, Fujita

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015009 on pSB1C3

Sequencing

Researcher: Mimata, Fujita, Kono

Part: BBa\_K2015000, BBa\_K2015009

Electrophoresis

Researcher: Mimata, Fujita

Part: BBa\_K2015011 on pSB1C3

Liquid Culture

Researcher: Kono

Part: BBa\_K2015000 on pSB1C3

Transformation

Researcher: Mimata

Part: BBa\_K2015011 on pSB1C3

Ethanol Precipitation

Researcher: Fujita

Part: BBa\_K2015000, BBa\_K2015009

Colony PCR

Researcher: Nishimura, Hirao, Kishimoto

Part: BBa\_K2015011

Digestion

Researcher: Nishimura, Hirao, Kishimoto

Part: BBa\_K2015009

Colony PCR

Researcher: Nishimura, Kishimoto

Part: BBa\_K2015009

Ethanol Precipitation

Researcher: Nishimura, Kishimoto

Part: BBa\_K2015009

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015009

Electrophoresis

Researcher: Esashi

Part: BBa\_K2015011

Mini-prep

Researcher: Mimata, Hirao

Part: BBa\_K2015008

Electrophoresis

Researcher: Noda

Part: BBa\_K2015008

Ligation

Researcher: Mimata, Hirao

Part: BBa\_K2015009 & pSB1C3

Mini-prep

Researcher: Mimata, Hirao

Part: BBa\_K2015000 on pSB1C3

Electrophoresis

Researcher: Noda

Part: BBa\_K2015000 on pSB1C3

PEG Precipitation

Researcher: Mimata, Hirao

Part: BBa\_K2015000 on pSB1C3

Electrophoresis

Researcher: Esashi

Part: BBa\_K2015009 on pSB1C3

Sequencing

Researcher: Mimata, Hirao

Part: BBa\_K2015009

Electrophoresis

Researcher: Hirao, Mimata

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015009 on pSB1C3

Transformation

Researcher: Mimata, Hirao

Part: BBa\_K2015009 on pSB1C3

2016/09/19

Sequencing

Researcher: Mimata, Hirao

Part: BBa\_K2015000

Colony PCR

Researcher: Mimata, Shibata, Hirao

Part: BBa\_K2015011

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015011

Liquid Culture

Researcher: Mimata

Part: BBa\_K2015009 on pSB1C3

Ethanol Precipitation

Researcher: Mimata, Shibata, Hirao

Part: BBa\_K2015000, BBa\_K2015009

Liquid Culture

Researcher: Nishimura

Part: BBa\_K2015011 on pSB1C3

PEG Precipitation

Researcher: Shibata

Part: BBa\_K2015008 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015008 on pSB1C3

Sequencing

Researcher: Shibata

Part: BBa\_K2015008

Ethanol Precipitation

Researcher: Nishimura

Part: BBa\_K2015008

Sequencing

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015009

PEG Precipitation

Researcher: Nishimura, Kishida

Part: BBa\_K2015000 on pSB1C3

Mini-prep

Researcher: Nishimura, Kishida

Part: BBa\_K2015011 on pSB1C3

Liquid Culture

Researcher: Nishimura

Part: BBa\_K2015000 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: BBa\_K2015000 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015011 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000 on pSB1C3

Ethanol Precipitation

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015009

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015011 on pSB1C3

Mini-prep

Researcher: Nishimura, Mimata

Part: BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Nishimura, Mimata

Part: BBa\_K2015009 on pSB1C3

PEG Precipitation

Researcher: Mimata, Nishimura

Part: BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015009 on pSB1C3

Colony PCR

Researcher: Mimata, Fujita

Part: BBa\_K2015008, BBa\_K2015009

Sequencing

Researcher: Mimata, Fujita

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015009

2016/09/20

Electrophoresis

Researcher: Mimata, Fujita

Part: BBa\_K2015008, BBa\_K2015009

Ethanol Precipitation

Researcher: Mimata, Fujita

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015009

Mini-prep

Researcher: Mimata, Fujita

Part: BBa\_K2015011 on pSB1C3

Colony PCR

Researcher: Mimata, Fujita

Part: BBa\_K2015008, BBa\_K2015009

Mini-prep

Researcher: Mimata, Fujita

Part: BBa\_K2015000 on pSB1C3

Electrophoresis

Researcher: Mimata, Fujita

Part: BBa\_K2015000 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015012, BBa\_K2015008

Digestion

Researcher: Nishimura

Part: BBa\_K2015012

Ethanol Precipitation

Researcher: Nishimura

Part: BBa\_K2015012

Colony PCR

Researcher: Nishimura

Part: BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015012

Ligation

Researcher: Nishimura

Part: BBa\_K2015012 & pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015008, BBa\_K2015009

Transformation

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015011 on pSB1C3, BBa\_K2015012 on pSB1A3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015011 on pSB1C3

Sequencing

Researcher: Fujita, Kono

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015009, BBa\_K2015012 - BBa\_K2015008

2016/09/21

PEG Precipitation

Researcher: Fujita, Kono

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015012 on pSB1A3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

Colony PCR

Researcher: Fujita, Kono

Part: BBa\_K2015008

Electrophoresis

Researcher: Fujita, Kono, Hirao

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015012 on pSB1A3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

Ethanol Precipitation

Researcher: Shibata, Mimata, Hirao

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015009, BBa\_K2015012 - BBa\_K2015008

Sequencing

Researcher: Mimata, Shibata, Hirao

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015012 - BBa\_K2015008

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015008

Ethanol Precipitation

Researcher: Shibata, Mimata, Hirao

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015012 - BBa\_K2015008

Liquid Culture

Researcher: Nishimura

Part: BBa\_K2015008 on pSB1C3

Colony PCR

Researcher: Nishimura, Kono

Part: BBa\_K2015009

PEG Precipitation

Researcher: Nishimura

Part: BBa\_K2015000

Electrophoresis

Researcher: Kono

Part: BBa\_K2015009

PEG Precipitation

Researcher: Mimata, Kono

Part: BBa\_K2015009

Electrophoresis

Researcher: Mimata, Kono

Part: BBa\_K2015000, BBa\_K2015009

Sequencing

Researcher: Mimata, Kono, Esashi

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015012 - BBa\_K2015008, BBa\_K2015009

2016/09/22

Ethanol Precipitation

Researcher: Mimata, Shibata, Noda

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015012 - BBa\_K2015008, BBa\_K2015009

Digestion

Researcher: Mimata

Part: BBa\_K2015012

Digestion

Researcher: Mimata

Part: BBa\_I13401

Ethanol Precipitation

Researcher: Mimata, Shibata

Part: BBa\_I13401, BBa\_K2015012 on pSB1A3

Electrophoresis

Researcher: Noda

Part: BBa\_I13401, BBa\_K2015012 on pSB1A3

Mini-prep

Researcher: Nishimura

Part: BBa\_K2015008 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015008 on pSB1A3

Transformation

Researcher: Nishimura

Part: BBa\_I13401 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: BBa\_K2015008 on pSB1C3

Sequencing

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015012 - BBa\_K2015008, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015008 on pSB1A3

Colony PCR

Researcher: Nishimura

Part: BBa\_K2015009

Ethanol Precipitation

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015012 - BBa\_K2015008, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Hirao, Nishimura

Part: BBa\_K2015009 on pSB1C3

Liquid Culture

Researcher: Nishimura, Hirao

Part: BBa\_K2015000 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: BBa\_K2015008 on pSB1C3

2016/09/23

Colony PCR

Researcher: Fujita, Shibata

Part: BBa\_K2015008

Digestion

Researcher: Nishimura, Shibata

Part: BBa\_K2015011, pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015008 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015011, pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015008

Ethanol Precipitation

Researcher: Shibata

Part: BBa\_K2015011

Transformation

Researcher: Nishimura

Part: BBa\_B0015 on pSB1C3

Ligation

Researcher: Shibata, Fujita

Part: BBa\_K2015011 & pSB1C3

Transformation

Researcher: Shibata

Part: BBa\_K2015011 on pSB1C3

Mini-prep

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1A3

Liquid Culture

Researcher: Nishimura

Part: BBa\_I13401 on pSB1A2

PEG Precipitation

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1A3

Colony PCR

Researcher: Nishimura

Part: BBa\_K2015011

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1A3

Mini-prep

Researcher: Nishimura, Kishimoto

Part: BBa\_K2015000 on pSB1C3

PEG Precipitation

Researcher: Nishimura, Kishida, Kishimoto

Part: BBa\_K2015000 on pSB1C3

Transformation

Researcher: Nishimura, Kishimoto

Part: BBa\_B0015 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015000 on pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015011

Liquid Culture

Researcher: Nishimura

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_K2015009 on pSB1C3

Digestion

Researcher: Nishimura

Part: BBa\_K2015012, pSB1C3

2016/09/24

Colony PCR

Researcher: Mimata

Part: BBa\_K2015011

Sequencing

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015008

Ethanol Precipitation

Researcher: Fujita

Part: BBa\_K2015012, pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015012, pSB1C3

Mini-prep

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_K2015009 on pSB1C3

PEG Precipitation

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_K2015009 on pSB1C3, pSB1C3

Digestion

Researcher: Nishimura

Part: pSB1C3

Digestion

Researcher: Mimata, Fujita

Part: BBa\_K2015012 on pSB1A3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Fujita, Mimata

Part: BBa\_K2015011

Liquid Culture

Researcher: Fujita

Part: BBa\_K2015000 on pSB1C3, BBa\_I13401 on pSB1A2

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Hirao, Shibata

Part: BBa\_K2015012 on pSB1A3, BBa\_K2015009 on pSB1C3

Ligation

Researcher: Hirao, Shibata

Part: BBa\_K2015012 & BBa\_K2015009 on pSB1C3, BBa\_K2015012 & pSB1C3

Electrophoresis

Researcher: Hirao, Shibata

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1C3, BBa\_K2015012 on pSB1C3

Transformation

Researcher: Kishida

Part: BBa\_K2015012 on pSB1C3, BBa\_B0015 on pSB1C3

Sequencing

Researcher: Nishimura

Part: BBa\_K2015000, BBa\_K2015011, BBa\_K2015008

Digestion

Researcher: Shibata, Nishimura

Part: BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015009 on pSB1C3

Liquid Culture

Researcher: Shibata

Part: BBa\_K2015011 on pSB1C3

2016/09/25

Ligation

Researcher: Shibata

Part: BBa\_K2015012 & BBa\_K2015009 on pSB1C3

Transformation

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1C3

Colony PCR

Researcher: Mimata

Part: BBa\_K2015011

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015011

Mini-prep

Researcher: Mimata, Fujita

Part: BBa\_K2015000 on pSB1C3, BBa\_I13401 on pSB1A2

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015000 on pSB1C3, BBa\_I13401 on pSB1A2

PEG Precipitation

Researcher: Nishimura, Fujita

Part: BBa\_K2015000 on pSB1C3, BBa\_I13401 on pSB1A2

PEG Precipitation

Researcher: Mimata, Fujita

Part: BBa\_K2015011 on pSB1C3

Transformation

Researcher: Mimata, Hirao

Part: BBa\_B0015 on pSB1C3, BBa\_I13401 on pSB1C3

PCR (2 STEP)

Researcher: Nishimura

Part: BBa\_I13401, BBa\_K2015012 - BBa\_K2015008

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015011

Colony PCR

Researcher: Mimata

Part: BBa\_K2015012

Electrophoresis

Researcher: Hirao

Part: BBa\_I13401, BBa\_K2015012 - BBa\_K2015008

Liquid Culture

Researcher: Nishimura

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015012

PCR (3 STEP)

Researcher: Nishimura

Part: BBa\_K2015009, BBa\_K2015012 - BBa\_K2015008

Liquid Culture

Researcher: Hirao

Part: BBa\_K2015012 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1C3

2016/09/26

Ligation

Researcher: Nishimura, Hirao

Part: BBa\_K2015012 & BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015012 - BBa\_K2015008, BBa\_K2015009

Transformation

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015008, BBa\_K2015009

Gel Extract

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015008, BBa\_K2015009

Transformation

Researcher: Mimata

Part: BBa\_B0015 on pSB1C3, BBa\_I13401 on pSB1C3

Mini-prep

Researcher: Mimata

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

PEG Precipitation

Researcher: Mimata

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Colony PCR

Researcher: Mimata, Shibata

Part: BBa\_K2015011

Digestion

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1C3, BBa\_I13401

PCR (3 STEP)

Researcher: Nishimura

Part: BBa\_I13401

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1A3, BBa\_I13401

Mini-prep

Researcher: Hirao, Shibata, Kishida

Part: BBa\_K2015012 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

Electrophoresis

Researcher: Noda

Part: BBa\_K2015011 on pSB1C3, BBa\_K2015012 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_I13401

Ethanol Precipitation

Researcher: Nishimura

Part: BBa\_I13401

PEG Precipitation

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

PEG Precipitation

Researcher: Nishimura

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

Digestion

Researcher: Nishimura

Part: pSB1C3

Liquid Culture

Researcher: Kishimoto

Part: BBa\_K2015011 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3, pSB1C3

Electrophoresis

Researcher: Nishimura, Kishimoto, Kono

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015012 on pSB1C3, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

Liquid Culture

Researcher: Nishimura, Kishimoto, Hirao

Part: BBa\_K2015012 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

2016/09/27

Digestion

Researcher: Mimata

Part: pSB1C3

Electrophoresis

Researcher: Mimata

Part: pSB1C3

Colony PCR

Researcher: Mimata

Part: BBa\_K2015012, BBa\_K2015009

Digestion

Researcher: Mimata

Part: pSB1C3

Transformation

Researcher: Nishimura, Mimata

Part: BBa\_B0015

Electrophoresis

Researcher: Mimata

Part: pSB1C3

Liquid Culture

Researcher: Nishimura

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015012, BBa\_K2015009

Digestion

Researcher: Mimata

Part: BBa\_I13401, BBa\_K2015012 on pSB1A3

Mini-prep

Researcher: Mimata

Part: BBa\_K2015011 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015011 on pSB1C3, BBa\_I13401, BBa\_K2015012 on pSB1A3

PEG Precipitation

Researcher: Mimata, Nishimura

Part: BBa\_K2015011 on pSB1C3

Liquid Culture

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1C3

Mini-prep

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_K2015012 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_K2015012 on pSB1C3, BBa\_K2015011 on pSB1C3

PEG Precipitation

Researcher: Mimata, Nishimura

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_K2015012 on pSB1C3

Gel Extract

Researcher: Nishimura

Part: BBa\_I13401

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_K2015012 on pSB1C3, BBa\_I13401

Sequencing

Researcher: Nishimura

Part: BBa\_K2015011, BBa\_K2015012

Ligation

Researcher: Shibata

Part: BBa\_K2015012 on pSB1A3 & BBa\_I13401

PEG Precipitation

Researcher: Fujita, Hirao, Kishimoto

Part: BBa\_K2015012 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_K2015011 on pSB1C3

Transformation

Researcher: Kishimoto, Hirao

Part: BBa\_K2015012 - BBa\_I13401 on pSB1A3

Liquid Culture

Researcher: Kishimoto, Hirao

Part: BBa\_K2015012 on pSB1C3, BBa\_K2015011 on pSB1C3

Electrophoresis

Researcher: Kishimoto, Hirao

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_K2015012 on pSB1C3, BBa\_K2015011 on pSB1C3

2016/09/28

Mini-prep

Researcher: Mimata

Part: BBa\_B0015 on pSB1C3, BBa\_K2015012 - BBa\_K2015009 on pSB1C3

PEG Precipitation

Researcher: Mimata, Nishimura

Part: BBa\_K2015012 - -BBa\_K2015009 on pSB1C3

Ethanol Precipitation

Researcher: Nishimura

Part: BBa\_K2015011, BBa\_K2015012

Liquid Culture

Researcher: Nishimura, Kishimoto, Kono

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Nishimura, Kishimoto, Kono

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1C3

Streaking (Single Colony Isolation)

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_I13401 on pSB1A3

Digestion

Researcher: Mimata, Fujita

Part: BBa\_K2015012 on pSB1A3, BBa\_K2015009

Mini-prep

Researcher: Kishida

Part: BBa\_K2015012 on pSB1C3, BBa\_K2015011 on pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa\_K2015012 on pSB1C3, BBa\_K2015011 on pSB1C3

Sequencing

Researcher: Nishimura

Part: BBa\_K2015011, BBa\_K2015012

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015012 on pSB1A3, BBa\_K2015009

Electrophoresis

Researcher: Kishida

Part: BBa\_K2015012 on pSB1C3, BBa\_K2015011 on pSB1C3

Ligation

Researcher: Fujita

Part: BBa\_K2015012 on pSB1A3 & BBa\_K2015009

Transformation

Researcher: Fujita

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1A3

2016/09/29

Ethanol Precipitation

Researcher: Nishimura

Part: BBa\_K2015011, BBa\_K2015012

Mini-prep

Researcher: Kono

Part: BBa\_B0015 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

Electrophoresis

Researcher: Mimata, Kono

Part: BBa\_B0015 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

PEG Precipitation

Researcher: Mimata, Kono

Part: BBa\_B0015 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

Streaking (Single Colony Isolation)

Researcher: Kono, Mimata

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1A3

Electrophoresis

Researcher: Shibata

Part: BBa\_B0015 on pSB1C3, BBa\_K2015012 - BBa\_K2015008 on pSB1A3

PCR (2 STEP)

Researcher: Nishimura

Part: BBa\_K2015013

Streaking (Single Colony Isolation)

Researcher: Hirao

Part: BBa\_K2015012 - BBa\_I13401 on pSB1A3

Electrophoresis

Researcher: Hirao, Kishida

Part: BBa\_K2015013

PCR (3 STEP)

Researcher: Nishimura, Hirao

Part: BBa\_K2015013

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015013

PCR (2 STEP)

Researcher: Nishimura

Part: BBa\_B0015

Electrophoresis

Researcher: Nishimura

Part: BBa\_B0015

Mini-prep

Researcher: Shibata

Part: BBa\_K2015012 - BBa\_I13401 on pSB1A3

PEG Precipitation

Researcher: Shibata

Part: BBa\_K2015012 - BBa\_I13401 on pSB1A3

2016/09/30

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015012 - BBa\_I13401 on pSB1A3

Digestion

Researcher: Nishimura

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_B0015 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_B0015 on pSB1C3

Gel Extract

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3

PCR (STEP DOWN)

Researcher: Mimata

Part: BBa\_K2015013

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa\_K2015013

Liquid Culture

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_I13401 on pSB1A3

PEG Precipitation

Researcher: Nishimura

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1C3, BBa\_B0015 on pSB1C3

Gel Extract

Researcher: Shibata

Part: BBa\_K2015012 - BBa\_K2015008

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1A3, BBa\_K2015012 - BBa\_K2015009 on pSB1C3, BBa\_B0015 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1C3, BBa\_B0015 on pSB1C3

2016/10/01

PCR (2 STEP)

Researcher: Mimata

Part: BBa\_K2015011

Digestion

Researcher: Mimata

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3, pSB1C3

Gel Free System

Researcher: Mimata, Kishimoto

Part: BBa\_J04500

PEG Precipitation

Researcher: Mimata, Kishimoto, Hirao

Part: BBa\_J04500 on pSB1C3

Electrophoresis

Researcher: Mimata, Kishimoto, Hirao

Part: BBa\_K2015011

Electrophoresis

Researcher: Mimata, Hirao

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3, pSB1C3

Electrophoresis

Researcher: Mimata, Hirao

Part: BBa\_J04500 on pSB1C3, BBa\_J04500, BBa\_K2015011

Digestion

Researcher: Hirao, Mimata

Part: BBa\_K2015011

Gel Free System

Researcher: Hirao, Mimata

Part: BBa\_J04500, BBa\_I13401

Transformation

Researcher: Kishida

Part: BBa\_R0010 on pSB1C3, BBa\_J04500 on pSB1C3

Liquid Culture

Researcher: Mimata, Shibata

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Digestion

Researcher: Hirao, Mimata

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Shibata, Hirao, Kishida

Part: pSB1C3, BBa\_K2015011, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Ethanol Precipitation

Researcher: Shibata

Part: BBa\_J04500, BBa\_I13401

Gel Extract

Researcher: Kishida

Part: pSB1C3

Electrophoresis

Researcher: Shibata

Part: pSB1C3, BBa\_J04500, BBa\_I13401

Digestion

Researcher: Shibata

Part: BBa\_J04500 on pSB1C3

Ligation

Researcher: Shibata

Part: pSB1C3 & BBa\_K2015011

Gel Free System

Researcher: Mimata

Part: BBa\_J04500, BBa\_I13401

Ethanol Precipitation

Researcher: Shibata

Part: BBa\_J04500, BBa\_I13401

Transformation

Researcher: Shibata

Part: BBa\_K2015011 on pSB1C3

2016/10/02

Digestion

Researcher: Shibata

Part: BBa\_J04500, BBa\_I13401

Electrophoresis

Researcher: Shibata

Part: BBa\_J04500 on pSB1C3, BBa\_J04500, BBa\_I13401

PEG Precipitation

Researcher: Shibata

Part: BBa\_J04500 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa\_J04500 on pSB1C3, BBa\_J04500, BBa\_I13401

Digestion

Researcher: Shibata

Part: BBa\_J04500 on pSB1C3

Digestion

Researcher: Shibata

Part: BBa\_K2015009 on pSB1C3, BBa\_J04500, BBa\_I13401

Mini-prep

Researcher: Shibata

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

PEG Precipitation

Researcher: Mimata

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa\_J04500 on pSB1C3, BBa\_K2015009 on pSB1C3, BBa\_J04500, BBa\_I13401, BBa\_K2015008 on pSB1C3

Digestion

Researcher: Mimata

Part: BBa\_J04500, BBa\_I13401, BBa\_I13401 on pSB1A2

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Digestion

Researcher: Mimata

Part: BBa\_K2015008 on pSB1C3

Transformation

Researcher: Mimata, Hirao

Part: BBa\_R0010 on pSB1C3, BBa\_J04500 on pSB1C3

Electrophoresis

Researcher: Mimata, Hirao

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3, BBa\_J04500, BBa\_I13401, BBa\_I13401 on pSB1A2

Electrophoresis

Researcher: Hirao, Fujita

Part: BBa\_J04500, BBa\_I13401

Gel Extract

Researcher: Hirao, Fujita

Part: BBa\_J04500, BBa\_I13401

Electrophoresis

Researcher: Hirao, Fujita

Part: BBa\_J04500, BBa\_I13401

Colony PCR

Researcher: Fujita, Hirao

Part: BBa\_K2015011

Ligation

Researcher: Fujita, Hirao

Part: BBa\_J04500 & BBa\_K2015008 on pSB1C3, BBa\_J04500 & BBa\_K2015009 on pSB1C3, BBa\_J04500 & BBa\_I13401 on pSB1A2,

Digestion

Researcher: Fujita, Kishimoto

Part: BBa\_I13401, BBa\_J04500

Transformation

Researcher: Mimata, Hirao, Fujita, Kishimoto

Part: BBa\_J04500 - BBa\_I13401 on pSB1A2, BBa\_J04500 - BBa\_K2015008 on pSB1C3, BBa\_J04500 - BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Kishimoto, Mimata, Fujita

Part: BBa\_J04500, BBa\_I13401

Electrophoresis

Researcher: Kishimoto, Fujita

Part: BBa\_K2015011

Electrophoresis

Researcher: Kono

Part: BBa\_J04500, BBa\_I13401

Gel Extract

Researcher: Kono

Part: BBa\_J04500, BBa\_I13401

2016/10/03

Electrophoresis

Researcher: Kono

Part: BBa\_J04500, BBa\_I13401

Streaking (Single Colony Isolation)

Researcher: Mimata

Part: BBa\_J04500 - BBa\_I13401 on pSB1A2

Colony PCR

Researcher: Mimata, Fujita

Part: BBa\_K2015011

Transformation

Researcher: Mimata, Fujita

Part: BBa\_J04500 on pSB1C3

Digestion

Researcher: Mimata, Fujita

Part: BBa\_I13401 on pSB1A2

Electrophoresis

Researcher: Fujita

Part: BBa\_I13401 on pSB1A2, BBa\_K2015011

Colony PCR

Researcher: Mimata, Fujita, Shibata

Part: BBa\_J04500 - BBa\_K2015009

Digestion

Researcher: Mimata, Fujita, Shibata

Part: BBa\_K2015011

Electrophoresis

Researcher: Fujita, Kono

Part: BBa\_K2015011

Liquid Culture

Researcher: Shibata

Part: BBa\_R0010 on pSB1C3

Ligation

Researcher: Fujita

Part: BBa\_K2015011 & pSB1C3, BBa\_J04500 & BBa\_K2015008 on pSB1C3

Electrophoresis

Researcher: Fujita, Kono

Part: BBa\_J04500 - BBa\_K2015009

Transformation

Researcher: Fujita

Part: BBa\_K2015011 on pSB1C3, BBa\_J04500 - BBa\_K2015008 on pSB1C3

Ligation

Researcher: Fujita

Part: BBa\_J04500 & BBa\_K2015008 on pSB1C3, BBa\_J04500 & BBa\_K2015009 on pSB1C3

2016/10/04

Streaking (Single Colony Isolation)

Researcher: Fujita

Part: BBa\_J04500 - BBa\_I13401 on pSB1A2

Mini-prep

Researcher: Fujita

Part: BBa\_R0010 on pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa\_R0010 on pSB1C3

PEG Precipitation

Researcher: Fujita, Mimata

Part: BBa\_R0010 on pSB1C3

Transformation

Researcher: Fujita, Mimata, Shibata

Part: BBa\_J04500 - BBa\_K2015008 on pSB1C3, BBa\_J04500 - BBa\_K2015009 on pSB1C3

Colony PCR

Researcher: Mimata, Shibata

Part: BBa\_K2015011, BBa\_J04500 - BBa\_K2015008

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa\_R0010 on pSB1C3

PCR (2 STEP)

Researcher: Mimata, Shibata

Part: BBa\_J04500

PEG Precipitation

Researcher: Mimata, Shibata

Part: BBa\_I13401 on pSB1A2

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa\_R0010 on pSB1C3, BBa\_I13401 on pSB1A2

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa\_J04500

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015011

Electrophoresis

Researcher: Shibata

Part: BBa\_J04500 - BBa\_K2015008

Colony PCR

Researcher: Fujita, Kono, Kishida

Part: BBa\_K2015011

Gel Extract

Researcher: Shibata

Part: BBa\_J04500

Electrophoresis

Researcher: Shibata

Part: BBa\_J04500

Digestion

Researcher: Fujita, Kishida

Part: BBa\_I13401 on pSB1A2, BBa\_J04500

Electrophoresis

Researcher: Shibata

Part: BBa\_I13401 on pSB1A2, BBa\_J04500

Electrophoresis

Researcher: Mimata, Fujita

Part: BBa\_K2015011

Ligation

Researcher: Mimata, Fujita, Kono

Part: BBa\_I13401 on pSB1A2 & BBa\_J04500

Streaking (Single Colony Isolation)

Researcher: Kishida, Mimata

Part: BBa\_J04500 - BBa\_I13401 on pSB1A2

2016/10/05

Colony PCR

Researcher: Mimata

Part: BBa\_K2015011

Transformation

Researcher: Mimata, Shibata

Part: BBa\_J04500 - BBa\_I13401 on pSB1A2, BBa\_J04500 on pSB1C3

Liquid Culture

Researcher: Mimata, Shibata

Part: BBa\_J04500 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015011

Colony PCR

Researcher: Mimata, Shibata

Part: BBa\_K2015011

PCR (2 STEP)

Researcher: Mimata, Shibata

Part: BBa\_E0040

Transformation

Researcher: Mimata, Shibata

Part: BBa\_B0034 on pSB1A2

Liquid Culture

Researcher: Mimata, Shibata

Part: BBa\_B0034 on pSB1A2

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015011, BBa\_E0040

2016/10/06

Mini-prep

Researcher: Mimata, Shibata

Part: BBa\_J04500 on pSB1C3, BBa\_I13401 on pSB1A2

Electrophoresis

Researcher: Mimata

Part: BBa\_J04500 on pSB1C3, BBa\_I13401 on pSB1A2

PEG Precipitation

Researcher: Mimata

Part: BBa\_J04500 on pSB1C3, BBa\_I13401 on pSB1A2

Electrophoresis

Researcher: Mimata

Part: BBa\_J04500 on pSB1C3, BBa\_I13401 on pSB1A2

PCR (2 STEP)

Researcher: Nishimura, Mimata

Part: BBa\_K2015011, BBa\_J04500, BBa\_I13401

Electrophoresis

Researcher: Mimata, Fujita

Part: BBa\_K2015011

PCR (2 STEP)

Researcher: Fujita, Mimata, Hirao

Part: BBa\_K2015011, BBa\_J04500

Gel Extract

Researcher: Hirao

Part: BBa\_I13401

Electrophoresis

Researcher: Hirao, Fujita

Part: BBa\_K2015011 on pSB1C3, BBa\_I13401

Liquid Culture

Researcher: Mimata, Fujita, Hirao

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Gel Extract

Researcher: Mimata

Part: BBa\_K2015011 on pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa\_J04500

2016/10/07

Gel Extract

Researcher: Nishimura

Part: BBa\_J04500

Electrophoresis

Researcher: Nishimura

Part: BBa\_J04500, BBa\_K2015011 on pSB1C3

Liquid Culture

Researcher: Nishimura

Part: BBa\_J04500 - BBa\_I13401 on pSB1A2

Digestion

Researcher: Nishimura

Part: BBa\_K2015011

Ligation

Researcher: Kishida, Fujita

Part: BBa\_K2015011

Mini-prep

Researcher: Mimata, Shibata

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

PEG Precipitation

Researcher: Mimata, Shibata

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015011

Digestion

Researcher: Fujita, Kishida, Shibata, Mimata

Part: BBa\_J04500 on pSB1C3, BBa\_K2015008, BBa\_K2015009, BBa\_I13401

Liquid Culture

Researcher: Nishimura

Part: BBa\_B0034 on pSB1A2, BBa\_J04500 - BBa\_I13401 on pSB1A2

Streaking (Single Colony Isolation)

Researcher: Nishimura

Part: BBa\_B0034 on pSB1A2, BBa\_J04500 - BBa\_I13401 on pSB1A2

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3, BBa\_J04500 on pSB1C3, BBa\_K2015008, BBa\_K2015009, BBa\_I13401

Transformation

Researcher: Fujita, Kishida, Nishimura

Part: BBa\_K2015011

Ethanol Precipitation

Researcher: Fujita

Part: BBa\_K2015000 on pSB1C3, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Ligation

Researcher: Shibata, Fujita, Kishida

Part: BBa\_J04500 on pSB1C3 & BBa\_K2015008, BBa\_J04500 on pSB1C3 & BBa\_K2015009, BBa\_J04500 on pSB1C3 & BBa\_I13401

Transformation

Researcher: Fujita

Part: BBa\_J04500 - BBa\_K2015008 on pSB1C3, BBa\_J04500 - BBa\_K2015009 on pSB1C3, BBa\_J04500 - BBa\_I13401on pSB1C3

2016/10/08

Liquid Culture

Researcher: Nishimura

Part: BBa\_J04500 - BBa\_I13401 on pSB1A2

Mini-prep

Researcher: Nishimura

Part: BBa\_B0034 on pSB1A2

PEG Precipitation

Researcher: Nishimura

Part: BBa\_B0034 on pSB1A2

Electrophoresis

Researcher: Nishimura

Part: BBa\_B0034 on pSB1A2

Colony PCR

Researcher: Shibata, Nishimura, Kishida

Part: BBa\_K2015011

PCR (3 STEP)

Researcher: Shibata

Part: BBa\_J04500

Transformation

Researcher: Kishida

Part: BBa\_J04500 on pSB1C3

PEG Precipitation

Researcher: Shibata

Part: BBa\_J04500 on pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa\_K2015011

Electrophoresis

Researcher: Shibata

Part: BBa\_J04500

Digestion

Researcher: Shibata

Part: BBa\_J04500 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa\_J04500 on pSB1C3

Digestion

Researcher: Mimata, Shibata

Part: BBa\_J04500 on pSB1C3

Ethanol Precipitation

Researcher: Mimata, Shibata

Part: BBa\_J04500 on pSB1C3

Liquid Culture

Researcher: Mimata, Shibata

Part: BBa\_J04500 - BBa\_I13401 on pSB1C3, BBa\_J04500 - BBa\_K2015009 on pSB1C3

PCR (2 STEP)

Researcher: Mimata, Shibata

Part: BBa\_R0010, BBa\_B0034

Ethanol Precipitation

Researcher: Mimata, Shibata

Part: BBa\_J04500 on pSB1C3, BBa\_R0010, BBa\_B0034

2016/10/09

Mini-prep

Researcher: Nishimura, Hirao

Part: BBa\_J04500 - BBa\_K2015009 on pSB1C3, BBa\_J04500 on pSB1C3, BBa\_J04500 - BBa\_I13401 on pSB1C3, BBa\_B0034 on pSB1A2

Electrophoresis

Researcher: Mimata

Part: BBa\_J04500 - BBa\_K2015009 on pSB1C3, BBa\_J04500 on pSB1C3, BBa\_J04500 - BBa\_I13401 on pSB1C3, BBa\_B0034 on pSB1A2

Colony PCR

Researcher: Nishimura

Part: BBa\_K2015011

Digestion

Researcher: Mimata

Part: BBa\_J04500 on pSB1C3

PEG Precipitation

Researcher: Mimata, Nishimura, Hirao

Part: BBa\_J04500 on pSB1C3, BBa\_B0034 on pSB1A2

Digestion

Researcher: Mimata

Part: BBa\_J04500 on pSB1C3

PCR (2 STEP)

Researcher: Hirao, Kono

Part: BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Hirao, Nishimura

Part: BBa\_J04500 on pSB1C3, BBa\_J04500, BBa\_B0034

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015011

Transformation

Researcher: Mimata, Shibata

Part: BBa\_I715039 on pSB1C3, BBa\_K741002 on pSB1C3, BBa\_J04450 on pSB1C3

Electrophoresis

Researcher: Hirao, Kono

Part: BBa\_K2015008, BBa\_K2015009

PCR (2 STEP)

Researcher: Nishimura

Part: BBa\_R0010 - BBa\_B0034

Electrophoresis

Researcher: Kono

Part: BBa\_K2015008, BBa\_K2015009

Gel Extract

Researcher: Kono

Part: BBa\_K2015008, BBa\_K2015009

Liquid Culture

Researcher: Shibata

Part: BBa\_K2015011 on pSB1C3

Electrophoresis

Researcher: Fujita, Mimata, Kono

Part: BBa\_K2015008, BBa\_K2015009, BBa\_R0010 - BBa\_B0034

2016/10/10

Mini-prep

Researcher: Fujita

Part: BBa\_K2015011 on pSB1C3

PEG Precipitation

Researcher: Fujita

Part: BBa\_K2015011 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015011 on pSB1C3

Digestion

Researcher: Fujita

Part: BBa\_K2015011 on pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015011 on pSB1C3

Digestion

Researcher: Nishimura

Part: BBa\_R0010, BBa\_B0034

Transformation

Researcher: Nishimura

Part: BBa\_K741002 on pSB1C3, BBa\_J04450 on pSB1C3

Electrophoresis

Researcher: Nishimura, Shibata

Part: BBa\_R0010, BBa\_B0034

Electrophoresis

Researcher: Nishimura, Shibata

Part: BBa\_R0010

Gel Extract

Researcher: Nishimura, Shibata

Part: BBa\_R0010

Ethanol Precipitation

Researcher: Nishimura

Part: BBa\_B0034

Electrophoresis

Researcher: Hirao

Part: BBa\_R0010, BBa\_B0034

Digestion

Researcher: Shibata, Hirao

Part: BBa\_R0010 on pSB1C3, BBa\_B0034 on pSB1A2

Ligation

Researcher: Nishimura

Part: BBa\_R0010 & BBa\_B0034 on pSB1C3

Transformation

Researcher: Nishimura

Part: BBa\_R0010 - BBa\_B0034 on pSB1A2

2016/10/11

PCR (2 STEP)

Researcher: Nishimura

Part: BBa\_R0010 - BBa\_B0034

PEG Precipitation

Researcher: Nishimura

Part: BBa\_R0010 on pSB1C3

Liquid Culture

Researcher: Nishimura

Part: BBa\_R0010 on pSB1C3

Transformation

Researcher: Nishimura

Part: BBa\_K880005 on pSB1C3

Ligation

Researcher: Shibata

Part: BBa\_R0010 & BBa\_B0034 on pSB1A2

Transformation

Researcher: Fujita

Part: BBa\_R0010 - BBa\_B0034 on pSB1A2

Mini-prep

Researcher: Mimata

Part: BBa\_R0010 on pSB1C3, BBa\_B0034 on pSB1C3

Electrophoresis

Researcher: Mimata

Part: BBa\_R0010 - BBa\_B0034, BBa\_R0010 on pSB1C3, BBa\_B0034 on pSB1A2

Transformation

Researcher: Mimata

Part: BBa\_K741002 on pSB1C3

PEG Precipitation

Researcher: Mimata, Kishida

Part: BBa\_R0010 on pSB1C3, BBa\_B0034 on pSB1A2

Electrophoresis

Researcher: Mimata, Kono, Kishida

Part: BBa\_R0010 on pSB1C3, BBa\_B0034 on pSB1A2

2016/10/12

Liquid Culture

Researcher: Shibata

Part: BBa\_K741002 on pSB1C3

Colony PCR

Researcher: Shibata, Mimata, Kishida

Part: BBa\_K2015011

Transformation

Researcher: Nishimura

Part: pET15b

Liquid Culture

Researcher: Shibata, Mimata

Part: BBa\_K2015011 on pSB1C3, BBa\_K2015012 on pSB1C3

Electrophoresis

Researcher: Kishimoto

Part: BBa\_K2015011

2016/10/13

PCR (3 STEP)

Researcher: Nishimura

Part: BBa\_K2015012

Mini-prep

Researcher: Nishimura, Kishimoto

Part: BBa\_K2015011 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa\_K2015012 on pSB1A2, BBa\_E0040

PEG Precipitation

Researcher: Shibata, Nishimura

Part: BBa\_K741002 on pSB1C3

Electrophoresis

Researcher: Nishimura, Hirao

Part: BBa\_K2015012 on pSB1A2, BBa\_E0040

Gel Extract

Researcher: Nishimura, Hirao

Part: BBa\_K2015012 on pSB1A2, BBa\_E0040

Mini-prep

Researcher: Kono, Nishimura

Part: BBa\_K2015012 on pSB1A2, BBa\_K2015012 on pSB1C3

PCR (3 STEP)

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1A2

Transformation

Researcher: Nishimura

Part: BBa\_E0040 on pSB1A2, BBa\_J04450 on pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa\_K741002 on pSB1C3

Liquid Culture

Researcher: Kishida

Part: pET15b

Electrophoresis

Researcher: Kishida, Kono

Part: BBa\_K2015012 on pSB1A2

2016/10/14

Digestion

Researcher: Nishumura

Part: BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1A2

Ethanol Precipitation

Researcher: Nishimura

Part: BBa\_K2015012

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1A2, BBa\_K2015008, BBa\_K2015009

Mini-prep

Researcher: Kono, Mimata

Part: pET15b

Electrophoresis

Researcher: Kono, Mimata

Part: pET15b

PEG Precipitation

Researcher: Kono, Mimata

Part: pET15b

PCR (2 STEP)

Researcher: Mimata

Part: BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Mimata

Part: pET15b, BBa\_K2015012, BBa\_K2015008, BBa\_K2015009

Ligation

Researcher: Fujita

Part: BBa\_K2015012 & BBa\_K2015008 on pSB1C3, BBa\_K2015012 & BBa\_K2015009 on pSB1C3

Digestion

Researcher: Mimata, Fujita, Shibata, Kono

Part: pET15b, BBa\_K2015008, BBa\_K2015009

Transformation

Researcher: Fujita

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1C3, BBa\_K2015012 - BBa\_K2015009 on pSB1C3

Streaking (Single Colony Isolation)

Researcher: Kono

Part: BBa\_E0040 on pSB1A2

Ethanol Precipitation

Researcher: Mimata, Fujita

Part: pET15b, BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Mimata, Fujita

Part: pET15b, BBa\_K2015008, BBa\_K2015009, BBa\_K2015011 on pSB1C3

PEG Precipitation

Researcher: Fujita

Part: BBa\_K2015011 on pSB1C3

2016/10/15

Digestion

Researcher: Nishimura

Part: pET15b

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015008, BBa\_K2015009

Gel Extract

Researcher: Nishimura

Part: BBa\_K2015008, BBa\_K2015009

Digestion

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1A2

Electrophoresis

Researcher: Fujita, Shibata

Part: BBa\_K2015011 on pSB1C3

Ethanol Precipitation

Researcher: Nihsimura, Fujita

Part: pET15b

Electrophoresis

Researcher: Nishimura

Part: pET15b, BBa\_K2015008, BBa\_K2015009, BBa\_E0040, BBa\_K2015012

Digestion

Researcher: Nishimura

Part: pET15b

Ethanol Precipitation

Researcher: Nihsimura

Part: pET15b

Digestion

Researcher: Nishimura

Part: BBa\_K2015009 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: pET15b

Electrophoresis

Researcher: Nishimura

Part: pET15b, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: pET15b

Digestion

Researcher: Nishimura

Part: BBa\_K2015000 on pSB1C3

Digestion

Researcher: Nishimura

Part: pET15b

Electrophoresis

Researcher: Nishimura

Part: pET15b, BBa\_K2015000 on pSB1C3

Streaking (Single Colony Isolation)

Researcher: Nishimura

Part: BBa\_E0040 on pSB1A2

Digestion

Researcher: Nishimura

Part: BBa\_E0040 on pSB1A2

Colony PCR

Researcher: Nishimura

Part: BBa\_K2015012 - BBa\_K2015009

Digestion

Researcher: Nishimura

Part: BBa\_K2015000 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_E0040 on pSB1A2

Liquid Culture

Researcher: Nishimura

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1C3

Digestion

Researcher: Nishimura

Part: pET15b

Electrophoresis

Researcher: Nishimura

Part: pET15b, BBa\_K2015000 on pSB1C3

Ligation

Researcher: Nishimura

Part: BBa\_K2015012 & BBa\_K2015008 on pSB1C3

Digestion

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1A2, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1A2, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Electrophoresis

Researcher: Hirao

Part: BBa\_K2015012 - BBa\_K2015009

PCR (3 STEP)

Researcher: Nishimura

Part: BBa\_K2015012, BBa\_E0040

Transformation

Researcher: Nishimura, Mimata

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1C3

Mini-prep

Researcher: Hirao

Part: pET15b

PEG Precipitation

Researcher: Hirao, Mimata

Part: pET15b

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015012 on pSB1A2, BBa\_K2015008 on pSB1C3, BBa\_K2015009 on pSB1C3

Liquid Culture

Researcher: Mimata

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1C3

PCR (2 STEP)

Researcher: Mimata

Part: BBa\_K741002

Ligation

Researcher: Mimata

Part: BBa\_K2015012 on pSB1A2 & BBa\_K2015008

Electrophoresis

Researcher: Mimata

Part: BBa\_K2015012 on pSB1A2, BBa\_K2015012, BBa\_E0040, BBa\_K741002, BBa\_R0010 - BBa\_B0034

2016/10/16

Transformation

Researcher: Fujita, nishimura

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1C3

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015012 on pSB1C3

Gel Extract

Researcher: Fujita

Part: BBa\_K2015012 on pSB1C3

Mini-prep

Researcher: Nishimura

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1C3

PCR (3 STEP)

Researcher: Mimata, Nishimura

Part: BBa\_K741002, BBa\_E0040

Transformation

Researcher: Mimata

Part: BBa\_E0040 on pSB1A2

Digestion

Researcher: Nishimura, Mimata

Part: pET15b, BBa\_K2015011 on pSB1C3, BBa\_K741002

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015012 on pSB1A2, BBa\_K2015012 - BBa\_K2015009 on pSB1C3, BBa\_K741002, BBa\_E0040, pET15b

Digestion

Researcher: Nishimura, Mimata

Part: pET15b, BBa\_K2015011 on pSB1C3

Liquid Culture

Researcher: Mimata, Shibata

Part: BBa\_K741002 on pSB1C3, BBa\_E0040 on pSB1A2, pET15b

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa\_K741002 on pSB1C3

Gel Extract

Researcher: Shibata, Hirao

Part:BBa\_K741002

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa\_K741002

2016/10/17

Digestion

Researcher: Nishimura, Shibata

Part: pET15b, BBa\_K2015011 on pSB1C3

Liquid Culture

Researcher: Fujita

Part: BBa\_K2015012 - BBa\_K2015009 on pSB1C3

Colony PCR

Researcher: Fujita

Part: BBa\_K2015012 - BBa\_K2015008

Liquid Culture

Researcher: Fujita

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015011 on pSB1C3, pET15b

Ligation

Researcher: Nishimura

Part: BBa\_K2015011 & BBa\_K741002 on pSB1C3

Digestion

Researcher: Nishimura

Part: pET15b

Electrophoresis

Researcher: Nishimura

Part: BBa\_K2015008, BBa\_K2015009, pET15b

Transformation

Researcher: Fujita, Nishimura

Part: BBa\_K2015011 - BBa\_K741002 on pSB1C3

Digestion

Researcher: Fujita

Part: BBa\_K2015008, BBa\_K2015009

Electrophoresis

Researcher: Fujita

Part: BBa\_K2015012 - BBa\_K2015008, BBa\_K2015011 - BBa\_K741002 on pSB1C3

Electrophoresis

Researcher: Fujita, Nishimura

Part: pET15b, BBa\_K2015008, BBa\_K2015009

Liquid Culture

Researcher: Nishimura

Part: BBa\_K2015011 on pSB1C3

Mini-prep

Researcher: Kishida

Part: pET15b, BBa\_K741002 on pSB1C3

PEG Precipitation

Researcher: Nishimura

Part: pET15b, BBa\_K741002 on pSB1C3

Transformation

Researcher: Nishimura

Part: BBa\_K2015008 on pET15b, BBa\_K2015009 on pET15b

Ethanol Precipitation

Researcher: Nishimura

Part: BBa\_K741002

Mini-prep

Researcher: Kishida

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa\_K2015012 - BBa\_K2015008 on pSB1C3, pET15b, BBa\_K741002 on pSB1C3

Ligation

Researcher: Nishimura

Part: BBa\_K2015008 & pET15b, BBa\_K2015009 & pET15b

Transformation

Researcher: Nishimura

Part: BBa\_K2015008 on pET15b, BBa\_K2015009 on pET15b

Liquid Culture

Researcher: Mimata, Hirao

Part: BBa\_K2015011 - BBa\_K741002 on pSB1C3

2016/10/18

Liquid Culture

Researcher: Mimata, Hirao

Part: BBa\_K2015011 - BBa\_K741002 on pSB1C3

Colony PCR

Researcher: Nishimura

Part: BBa\_K2015011 - BBa\_K741002 on pET15b

Mini-prep

Researcher: Kono

Part: BBa\_K2015011 - BBa\_K741002 on pSB1C3

PEG Precipitation

Researcher: Kono

Part: BBa\_K2015011 - BBa\_K741002 on pSB1C3

Electrophoresis

Researcher: Kishida

Part: BBa\_K2015011 - BBa\_K741002 on pSB1C3

Electrophoresis

Researcher: Kono

Part: BBa\_K2015011 - BBa\_K741002 on pSB1C3

2016/10/19

Colony PCR

Researcher: Nishimura, Shibata

Part: BBa\_K2015011 - BBa\_K741002 on pSB1C3

Ligation

Researcher: Nishimura

Part: BBa\_K2015008 & BBa\_K2015009 on pET15b