iGEM 2021 — Team XHD-Wuhan-Pro-China

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# Team:XHD-Wuhan-Pro-China/Notebook

![](https://static.igem.org/mediawiki/2021/7/7a/T--XHD-Wuhan-Pro-China--TeamLogo.png)

Notebook

[Notebook](https://static.igem.org/mediawiki/2021/8/85/T--XHD-Wuhan-Pro-China--Notebook.pdf)

![logo](https://static.igem.org/mediawiki/2021/7/7a/T--XHD-Wuhan-Pro-China--TeamLogo.png)
![](https://static.igem.org/mediawiki/2021/thumb/1/1d/T--XHD-Wuhan-Pro-China--SoberUp.png/120px-T--XHD-Wuhan-Pro-China--SoberUp.png)

Copyright ©2021 iGEM XHD-Wuhan-Pro-China All rights reserved

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# Team:XHD-Wuhan-Pro-China/Engineering

![](https://static.igem.org/mediawiki/2021/7/7a/T--XHD-Wuhan-Pro-China--TeamLogo.png)

Engineering

![](https://static.igem.org/mediawiki/2021/3/38/IGEM_Eng_Cycle.png)

The Initial Design-The Production Module

Initially, when our team analyzed the problem of alcohol poisoning and enzyme dysfunction。 we found that the digestion of alcohol is controlled by two major enzymes, ADH and ALDH. ADH converts alcohol to acetaldehyde, and ALDH converts acetaldehyde to acetic acid. A malfunction of any of these two enzymes could result in toxic substances building up in our body. However, 2 major gene mutations in the east Asian body enhances the function of ADH and weakens the power of ALDH. As a result, the toxic acetaldehyde easily builds up in east Asian’s bodies when they drink alcohol. Such phenomenon is so called “the alcohol blushing syndrome” as the acetaldehyde makes your face turn red. Therefore, while constructing our plasmid, we first inserted the genes for these two enzymes, adh and aldh gene, making up the production module of our final design.

![](https://static.igem.org/mediawiki/2021/6/6e/T--XHD-Wuhan-Pro-China--05.jpg)

Improvements Made-The Acceleration Module

After we came up with the initial design, we were fortunate enough to have the chance to meet professor Ma, a professor at Huazhong Agriculture University. After listening to our plan, he first showed interest in the project, and then pointed out a few potential problems in it. Within these questions, one of them showed that in the alcohol metabolism process, NADH is converted to NAD+, which disrupts the energy balance in the cell bodies.

After identifying the problem, we tested the actual situation in experimental settings. In our experiments, we found out that the unbalance of NADH and NAD+ does significantly limit the rate of metabolism process. To solve the issue, we also inserted NADE and NOX genes from E.coli k12 and Lactobacillus brevis to overcome the energy unbalance for speeding up the reactions as well as promoting the efficiency of the enzymes. This forms our energy module or acceleration module.

![](https://static.igem.org/mediawiki/2021/e/e9/T--XHD-Wuhan-Pro-China--06.jpg)

After inserting the acceleration module to create plasmid PSB-AN, we went through a series of experiments to test if the module had increased the efficiency of alcohol digestion. Results of the trials shown that both PSB-AA and PSB-AN had effectively decreased the alcohol concentration in experimental settings, with the improved module enhancing the digestive abilities of the enzymes.

![](https://static.igem.org/mediawiki/2021/3/37/T--XHD-Wuhan-Pro-China--07.jpg)

With these two parts combined together, alcohol in the body can be converted to harmless acetic acid efficiently and safely.

Last part

Unfortunately, as a group of high school students, math is a little bit difficult for us, and we need more time to research and study. At this time, we are not able to publish the results.

In this way, we will continue to work hard, and strive to show the results of the digital simulation at the time of the final defense.

![logo](https://static.igem.org/mediawiki/2021/7/7a/T--XHD-Wuhan-Pro-China--TeamLogo.png)

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# Team:XHD-Wuhan-Pro-China/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2019 Nantes](https://2019.igem.org/Team%3ANantes/Experiments)
* [2019 TU Eindhoven](https://2019.igem.org/Team%3ATU_Eindhoven/Experiments)
* [2019 Mingdao](https://2019.igem.org/Team%3AMingdao/Demonstrate)
* [2020 Amsterdam](https://2020.igem.org/Team%3AAmsterdam/Experiments)
* [2020 NCTU Formosa](https://2020.igem.org/Team%3ANCTU_Formosa/Experiments)
* [2020 USAFA](https://2020.igem.org/Team%3AUSAFA/Experiments)

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:XHD-Wuhan-Pro-China/Results

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2019 Newcastle](https://2019.igem.org/Team%3ANewcastle/Results)
* [2019 Munich](https://2019.igem.org/Team%3AMunich/Results)
* [2019 Tec Chihuahua](https://2019.igem.org/Team%3ATec-Chihuahua/Results)
* [2020 Aalto Helsinki](https://2020.igem.org/Team%3AAalto-Helsinki/Results)
* [2020 GreatBay SCIE](https://2020.igem.org/Team%3AGreatBay_SCIE/Results)
* [2020 Queens Canada](https://2020.igem.org/Team%3AQueens_Canada/Results)