iGEM 2018 — Team KAIT_JAPAN

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:KAIT JAPAN/Notebook

[![KAIT JAPAN](https://static.igem.org/mediawiki/2018/thumb/4/46/T--KAIT_JAPAN--logo.png/320px-T--KAIT_JAPAN--logo.png)](/Team%3AKAIT_JAPAN)

* Project

  [Overview](/Team%3AKAIT_JAPAN/Project)
  [Design](/Team%3AKAIT_JAPAN/Design)
  [Results](/Team%3AKAIT_JAPAN/Results)
  [Safety](/Team%3AKAIT_JAPAN/Safety)
  [Interlab](/Team%3AKAIT_JAPAN/InterLab)
* [Parts](/Team%3AKAIT_JAPAN/parts/overview)
* Notebook

  [Lab note](/Team%3AKAIT_JAPAN/notebook/lab_note)
  [Protocols](/Team%3AKAIT_JAPAN/notebook/protocols)
* Human Practices

  [Human Practices](/Team%3AKAIT_JAPAN/Human_Practices)
  [Integrated Human Practices](/Team%3AKAIT_JAPAN/Human_Practices/achievements)
* [Collaborations](/Team%3AKAIT_JAPAN/Collaborations)
* Teams

  [Members](/Team%3AKAIT_JAPAN/teams/members)
  [Sponsors](/Team%3AKAIT_JAPAN/teams/sponsors)
  [Attributions](/Team%3AKAIT_JAPAN/Attributions)
  [Achievements](/Team%3AKAIT_JAPAN/achievements)

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:KAIT JAPAN/Experiments

[![KAIT JAPAN](https://static.igem.org/mediawiki/2018/thumb/4/46/T--KAIT_JAPAN--logo.png/320px-T--KAIT_JAPAN--logo.png)](/Team%3AKAIT_JAPAN)

* Project

  [Overview](/Team%3AKAIT_JAPAN/Project)
  [Design](/Team%3AKAIT_JAPAN/Design)
  [Results](/Team%3AKAIT_JAPAN/Results)
  [Safety](/Team%3AKAIT_JAPAN/Safety)
  [Interlab](/Team%3AKAIT_JAPAN/InterLab)
* [Parts](/Team%3AKAIT_JAPAN/parts/overview)
* Notebook

  [Lab note](/Team%3AKAIT_JAPAN/notebook/lab_note)
  [Protocols](/Team%3AKAIT_JAPAN/notebook/protocols)
* Human Practices

  [Human Practices](/Team%3AKAIT_JAPAN/Human_Practices)
  [Integrated Human Practices](/Team%3AKAIT_JAPAN/Human_Practices/achievements)
* [Collaborations](/Team%3AKAIT_JAPAN/Collaborations)
* Teams

  [Members](/Team%3AKAIT_JAPAN/teams/members)
  [Sponsors](/Team%3AKAIT_JAPAN/teams/sponsors)
  [Attributions](/Team%3AKAIT_JAPAN/Attributions)
  [Achievements](/Team%3AKAIT_JAPAN/achievements)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:KAIT JAPAN/Results

[![KAIT JAPAN](https://static.igem.org/mediawiki/2018/thumb/4/46/T--KAIT_JAPAN--logo.png/320px-T--KAIT_JAPAN--logo.png)](/Team%3AKAIT_JAPAN)

* Project

  [Overview](/Team%3AKAIT_JAPAN/Project)
  [Design](/Team%3AKAIT_JAPAN/Design)
  [Results](/Team%3AKAIT_JAPAN/Results)
  [Safety](/Team%3AKAIT_JAPAN/Safety)
  [Interlab](/Team%3AKAIT_JAPAN/InterLab)
* [Parts](/Team%3AKAIT_JAPAN/parts/overview)
* Notebook

  [Lab note](/Team%3AKAIT_JAPAN/notebook/lab_note)
  [Protocols](/Team%3AKAIT_JAPAN/notebook/protocols)
* Human Practices

  [Human Practices](/Team%3AKAIT_JAPAN/Human_Practices)
  [Integrated Human Practices](/Team%3AKAIT_JAPAN/Human_Practices/achievements)
* [Collaborations](/Team%3AKAIT_JAPAN/Collaborations)
* Teams

  [Members](/Team%3AKAIT_JAPAN/teams/members)
  [Sponsors](/Team%3AKAIT_JAPAN/teams/sponsors)
  [Attributions](/Team%3AKAIT_JAPAN/Attributions)
  [Achievements](/Team%3AKAIT_JAPAN/achievements)

# KAIT\_JAPAN

### Overview

We planned to make 4 new basic parts. We were able to complete two parts out of them. Two things that could be completed are BBa\_K 2821002 Promoter (RhlR + C4 - HSL inhibited - 1), BBa\_K 2821003 Promoter (RhlR + C4 - HSL inhibited - 2).

### PCR

As a result of performing colony PCR after ligation, it was judged that the ligation of BBa\_K 2821002, BBa\_K 2821003 was successful since the band appeared at the total position of the spacer sequence and the target sequence.
(left1~8: BBa\_K 2821002 Promoter (RhlR + C4 - HSL inhibited - 1).)
(Right1~8: BBa\_K 2821003 Promoter (RhlR + C4 - HSL inhibited - 2).)

![RHLRBS](https://static.igem.org/mediawiki/2018/7/7b/T--KAIT_JAPAN--RhlRBS_colonyPCR.jpg)