iGEM 2019 — Team BUAP_Mexico

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:BUAP Mexico/Notebook

![](https://static.igem.org/mediawiki/2019/3/3c/Igem_mobile_menu_logo.svg)

![](https://static.igem.org/mediawiki/2019/e/ee/2019_team_menu_icon.svg)

[![](https://placehold.it/350x150/ececec/cecece)](https://2019.igem.org/Team%3ABUAP_Mexico)
[HOME](https://2019.igem.org/Team%3ABUAP_Mexico)

TEAM

[Team Members](https://2019.igem.org/Team%3ABUAP_Mexico/Team)
[Collaborations](https://2019.igem.org/Team%3ABUAP_Mexico/Collaborations)

PROJECT

[Description](https://2019.igem.org/Team%3ABUAP_Mexico/Description)
[Design](https://2019.igem.org/Team%3ABUAP_Mexico/Design)
[Experiments](https://2019.igem.org/Team%3ABUAP_Mexico/Experiments)
[Notebook](https://2019.igem.org/Team%3ABUAP_Mexico/Notebook)
[Contribution](https://2019.igem.org/Team%3ABUAP_Mexico/Contribution)
[Results](https://2019.igem.org/Team%3ABUAP_Mexico/Results)
[Demonstrate](https://2019.igem.org/Team%3ABUAP_Mexico/Demonstrate)
[Improve](https://2019.igem.org/Team%3ABUAP_Mexico/Improve)
[Attributions](https://2019.igem.org/Team%3ABUAP_Mexico/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3ABUAP_Mexico/Parts)
[Basic Parts](https://2019.igem.org/Team%3ABUAP_Mexico/Basic_Part)
[Composite Parts](https://2019.igem.org/Team%3ABUAP_Mexico/Composite_Part)
[Part Collection](https://2019.igem.org/Team%3ABUAP_Mexico/Part_Collection)

[SAFETY](https://2019.igem.org/Team%3ABUAP_Mexico/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3ABUAP_Mexico/Human_Practices)
[Education & Engagement](https://2019.igem.org/Team%3ABUAP_Mexico/Public_Engagement)

AWARDS

[Entrepreneurship](https://2019.igem.org/Team%3ABUAP_Mexico/Entrepreneurship)
[Hardware](https://2019.igem.org/Team%3ABUAP_Mexico/Hardware)
[Measurement](https://2019.igem.org/Team%3ABUAP_Mexico/Measurement)
[Model](https://2019.igem.org/Team%3ABUAP_Mexico/Model)
[Plant](https://2019.igem.org/Team%3ABUAP_Mexico/Plant)
[Software](https://2019.igem.org/Team%3ABUAP_Mexico/Software)

[JUDGING FORM ⇗](https://igem.org/2019_Judging_Form?team=BUAP_Mexico)

# Notebook

## June

* Collaboration. 28th- Meeting with team UAAAN through video call

### July

* Collaboration. 10th- Team UAAAN
* Education. 27th- RushGaming workshop participation to develop a software to teach synthetic biology.
* Human Practices. 29th- Meeting with Nora Yessica Merino escamilla congresswoman to introduce her pur project and define our goals.

#### August

* Collaboration. 12th- Team UAAAN.
* Human Practices. 14th- Video call with Lic. Osvaldo Sastré Gómez, we considered to review the mexican laws about women scientist and make a purpose of update.
* Public engagement. 26th - Green carson meeting to talk about the problems with plastics and how people could recycle it.
* Human Practices. 28th- Meeting with Nora Yessica Merino escamilla congresswoman to present her our law actualization.
* Human practices. 30- 31th - Living the science workshop: Women in Science.

##### September

* Collaboration. 6th- Team UAAAN.
* Collaboration. 7th- Team UAAAN.
* Public engagement 7- 8th - Living the science workshop: Talking about environment and how to solve the plastic and air pollution troubles doing simple actions and through synthetic biology.
* Human Practices. 16th- Video call with Lic. Osvaldo Sastré Gómez.
* Collaboration 16th- Team UAAAN
* Human Practices. 23th- Meeting with Nora Yessica Merino escamilla congresswoman to get the last feedback about legislation and presentation of articles updated about scientist woman and their legal rights.
* Public Engagement: 26th Meeting with Green Carson specialist to talk about our project and how to use it to improve the conscience about recycle in Puebla’s community starting with the Benemérita Universidad Autónoma de Puebla’s alumni.
* Collaboration. 29th- Team UAAAN video call.
* Collaboration. 30th- Team UAAAN video call
* Collaboration. 31th- Team UAAAN video call.

###### October

* Human Practices. 8th- Video call with Lic. Osvaldo Sastré Gómez.
* Human Practices: 9th- Ph.D. Abigail Martínez Torres.
* Human Practices: 10th- Ph. D. Elsa Iracena Castañeda Roldan.
* Human Practices: 11th- Ph.D. Etelvina Gándara Zamorano.
* Human Practices. 14th- Meeting with MC. Xóchtil Arteaga Villamil. Last feedback about women in science work.
* Education 13th - Living the science workshop: Scientific illustration.


[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:BUAP Mexico/Experiments

![](https://static.igem.org/mediawiki/2019/3/3c/Igem_mobile_menu_logo.svg)

![](https://static.igem.org/mediawiki/2019/e/ee/2019_team_menu_icon.svg)

[![](https://placehold.it/350x150/ececec/cecece)](https://2019.igem.org/Team%3ABUAP_Mexico)
[HOME](https://2019.igem.org/Team%3ABUAP_Mexico)

TEAM

[Team Members](https://2019.igem.org/Team%3ABUAP_Mexico/Team)
[Collaborations](https://2019.igem.org/Team%3ABUAP_Mexico/Collaborations)

PROJECT

[Description](https://2019.igem.org/Team%3ABUAP_Mexico/Description)
[Design](https://2019.igem.org/Team%3ABUAP_Mexico/Design)
[Experiments](https://2019.igem.org/Team%3ABUAP_Mexico/Experiments)
[Notebook](https://2019.igem.org/Team%3ABUAP_Mexico/Notebook)
[Contribution](https://2019.igem.org/Team%3ABUAP_Mexico/Contribution)
[Results](https://2019.igem.org/Team%3ABUAP_Mexico/Results)
[Demonstrate](https://2019.igem.org/Team%3ABUAP_Mexico/Demonstrate)
[Improve](https://2019.igem.org/Team%3ABUAP_Mexico/Improve)
[Attributions](https://2019.igem.org/Team%3ABUAP_Mexico/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3ABUAP_Mexico/Parts)
[Basic Parts](https://2019.igem.org/Team%3ABUAP_Mexico/Basic_Part)
[Composite Parts](https://2019.igem.org/Team%3ABUAP_Mexico/Composite_Part)
[Part Collection](https://2019.igem.org/Team%3ABUAP_Mexico/Part_Collection)

[SAFETY](https://2019.igem.org/Team%3ABUAP_Mexico/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3ABUAP_Mexico/Human_Practices)
[Education & Engagement](https://2019.igem.org/Team%3ABUAP_Mexico/Public_Engagement)

AWARDS

[Entrepreneurship](https://2019.igem.org/Team%3ABUAP_Mexico/Entrepreneurship)
[Hardware](https://2019.igem.org/Team%3ABUAP_Mexico/Hardware)
[Measurement](https://2019.igem.org/Team%3ABUAP_Mexico/Measurement)
[Model](https://2019.igem.org/Team%3ABUAP_Mexico/Model)
[Plant](https://2019.igem.org/Team%3ABUAP_Mexico/Plant)
[Software](https://2019.igem.org/Team%3ABUAP_Mexico/Software)

[JUDGING FORM ⇗](https://igem.org/2019_Judging_Form?team=BUAP_Mexico)

# Here you can download some experiments that we did.[![Electrophoresis]( "Zymo support")](https://static.igem.org/mediawiki/2019/d/d1/T--BUAP_Mexico--ELECTROPHORESIS_PROTOCOL.pdf) [![DNA recovery]( "Zymo support")](https://static.igem.org/mediawiki/2019/b/bd/T--BUAP_Mexico--FRAGMENT_DNA_RECOVERY_%28ZYMO%2C_D4045%29.pdf) [![Competent cells]( "Zymo support")](https://static.igem.org/mediawiki/2019/a/a2/T--BUAP_Mexico--MAKING_CALCIUM_COMPETENT_CELLS.pdf) [![Miniprep]( "Zymo support")](https://static.igem.org/mediawiki/2019/7/70/T--BUAP_Mexico--PLASMID_MINIPREP_%28ZYMO%2C_D4209%29.pdf) [![Transformation]( "Zymo support")](https://static.igem.org/mediawiki/2019/6/6e/T--BUAP_Mexico--TRANSFORMATION_COMPETENTS_CELL.pdf) [![Restriction]( "Zymo support")](https://static.igem.org/mediawiki/2019/9/9c/T--BUAP_Mexico--RESTRICTION_PROTOCOL.pdf)Aanerobic conditions test In order to test the PHB synthesis rate in E. coli under anaerobic and aerobic conditions as well as the bacterial growth, E. coli BL-21 was transformed using the biobrick BBa\_K934001 and was cultured in TB medium. Aerobic and anaerobic media were inoculated with pyruvate 50mM to simulate the real conditions under our bacteria would work. This quantity was used because is necessary to suppress the pyruvate dehydrogenase complex repressor (pdhR) ensuring the theoretical maximum expression rate of pyruvate dehydrogenase complex which allows the metabolism of pyruvate into acetyl-coA, the first substrate in PHB synthesis. Each 24 hours we measured the bacterial growth of both media and after 72 hours the bacterial culture was centrifugate until get a pellet, which wasstored at -20°C during 72 hrs, after this time chloroform was added to get the PHB. The measure of PHB production showed a production of 25mg in media with oxygen and 90mg of PHB under anaerobic conditions. ![](https://static.igem.org/mediawiki/2019/3/3c/T--BUAP_Mexico--production.jpg) **Figure 1:** PHB production under aerobic and anaerobic conditions

![](https://static.igem.org/mediawiki/2019/4/48/T--BUAP_Mexico--BGrowth.jpg)

**Figure 2:** Bacterial growth under anaerobic and aerobic conditions

![](https://static.igem.org/mediawiki/parts/thumb/a/a5/T--BUAP_Mexico--PHB.jpg/800px-T--BUAP_Mexico--PHB.jpg)

**Figure 3:** PHB obtained with chloroform treatment

### Conclusion

The result showed that PHB in E. coli works better under anaerobic contions even if the number of microorganism is less tha the aerobic conditions. We think this biobrick would work fine in our theoretical conditions where there are no oxygen and exist a high concentration of pytuvate, in our experiment we confirm this molecule enhances the PHB production because under high concentration it repress genes related to aerobic pathways, in this conditions the pyruvate obtained is metabolized in PHB.

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:BUAP Mexico/Results