iGEM 2009 — Team IPN-UNAM-Mexico

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# Team:IPN-UNAM-Mexico/Notebook

### From 2009.igem.org

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| --- | --- | --- | --- | --- | --- | --- | --- | --- |
| [HOME](https://2009.igem.org/Team%3AIPN-UNAM-Mexico/Front) | [PROJECT](https://2009.igem.org/Team%3AIPN-UNAM-Mexico/Project "Know more about our project") | [TEAM](https://2009.igem.org/Team%3AIPN-UNAM-Mexico/Team "Meet the people :D") | [PARTS](https://2009.igem.org/Team%3AIPN-UNAM-Mexico/Parts "Learn about the parts used") | [MODELLING](https://2009.igem.org/Team%3AIPN-UNAM-Mexico/Modeling "Learn more about how we make the models") | [NOTEBOOK](https://2009.igem.org/Team%3AIPN-UNAM-Mexico/Notebook "Our notebook") | [RESULTS](https://2009.igem.org/Team%3AIPN-UNAM-Mexico/Results "What we get") | [COLLABORATION](https://2009.igem.org/Team%3AIPN-UNAM-Mexico/Collaboration "Because all we need some help :)") | [FUTURE](https://2009.igem.org/Team%3AIPN-UNAM-Mexico/Future "Never stop thinking, try to go far always") |

# NOTEBOOK

As summer proyect our experimental work began at july the 7th 2009 using 2008 bioparts catalog, unfortunately it didn't work properly, so we had a lot of trouble to take out DNA and transform into *E. coli*. For this reason we had to delay lab work and request the 2009 catalog.

# [![Month-icon.png](/wiki/images/3/35/Month-icon.png)](/File%3AMonth-icon.png)[Protocols](/Team%3AIPN-UNAM-Mexico/Protocols "Team:IPN-UNAM-Mexico/Protocols")

## [![Month-icon.png](/wiki/images/3/35/Month-icon.png)](/File%3AMonth-icon.png)[July](/Team%3AIPN-UNAM-Mexico/Notebook/July "Team:IPN-UNAM-Mexico/Notebook/July")

## [![Month-icon.png](/wiki/images/3/35/Month-icon.png)](/File%3AMonth-icon.png)[August](/Team%3AIPN-UNAM-Mexico/Notebook/August "Team:IPN-UNAM-Mexico/Notebook/August")

## [![Month-icon.png](/wiki/images/3/35/Month-icon.png)](/File%3AMonth-icon.png)[September](/Team%3AIPN-UNAM-Mexico/Notebook/September "Team:IPN-UNAM-Mexico/Notebook/September")

## [![Month-icon.png](/wiki/images/3/35/Month-icon.png)](/File%3AMonth-icon.png)[October](/Team%3AIPN-UNAM-Mexico/Notebook/October "Team:IPN-UNAM-Mexico/Notebook/October")

# [![Month-icon.png](/wiki/images/3/35/Month-icon.png)](/File%3AMonth-icon.png)[Safety](/Team%3AIPN-UNAM-Mexico/Safety "Team:IPN-UNAM-Mexico/Safety")

Our synthetic construction consists of 29 bioparts, which needed 12 ligations and different strategies to make it functional as is described below:

| Number | Biobrick | Restriction |
| --- | --- | --- |
| 2 | BBa\_R0079 | S & P |
| 3 | BBa\_F1610 | X & P |
| 4 | BBa\_K091146 | S & P |
| 5 | BBa\_K093005 | X & |
| 6 | BBa\_K081016 | X & P |
| 7 | BBa\_EC840 | X & P |
| 8 | BBa\_K081009 | X & P |
| 9 | BBa\_R0051 | S &P |
| 10 | BBa\_J06800 | X & P |
| 11 | BBa\_Q04121 | X & P |
| 12 | BBa\_B0034 | S & P |
| 13 | BBa\_C0079 | E & S |
| 14 | BBa\_C0179 | E & S |
| 15 | BBa\_B0015 | E & X |
| 16 | BBa\_K081018 | E & S |
| 17 | BBa\_K116640 | X & P |
| 23 | BBa\_S03154 | X & P |
| 24 | BBa\_k145201 | E & S |

| Ligation | Nickname | Biobrick |
| --- | --- | --- |
| 4-23 | Ciencias I | BBa\_K226005 |
| 13-15 | Ciencias II | BBa\_K266002 |
| Ciencias I-7 | Ciencias III | BBa\_K266006 |
| 24-17 | Ciencias IV | BBa\_K266001 |
| 2-3 | Ciencias V | BBa\_K266000 |
| J231000-11 | Ciencias VI | BBa\_K266008 |
| Amplicon - Ciencias II | Ciencias VII | BBa\_K266003 |
| Ciencias VI - 12 | Ciencias VIII | BBa\_K266009 |
| Ciencias III - 19 | Ciencias IX | BBa\_K266006 |
| J23100 – Ciencias VII | Ciencias X | BBa\_K266004 |
| Ciencias III - Ciencias V | Ciencias XI | BBa\_K266007 |
| Ciencias X - Ciencias IV | Ciencias XII | BBa\_K266010 |

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