iGEM 2018 — Team SMS_Shenzhen

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# Team:SMS Shenzhen/Notebook

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[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3ASMS_Shenzhen)
[HOME](https://2018.igem.org/Team%3ASMS_Shenzhen)

TEAM

[Team Members](https://2018.igem.org/Team%3ASMS_Shenzhen/Team)
[Collaborations](https://2018.igem.org/Team%3ASMS_Shenzhen/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ASMS_Shenzhen/Description)
[Design](https://2018.igem.org/Team%3ASMS_Shenzhen/Design)
[Experiments](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments)
[Notebook](https://2018.igem.org/Team%3ASMS_Shenzhen/Notebook)
[InterLab](https://2018.igem.org/Team%3ASMS_Shenzhen/InterLab)
[Model](https://2018.igem.org/Team%3ASMS_Shenzhen/Model)
[Results](https://2018.igem.org/Team%3ASMS_Shenzhen/Results)
[Demonstrate](https://2018.igem.org/Team%3ASMS_Shenzhen/Demonstrate)
[Improve](https://2018.igem.org/Team%3ASMS_Shenzhen/Improve)
[Attributions](https://2018.igem.org/Team%3ASMS_Shenzhen/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ASMS_Shenzhen/Parts)
[Basic Parts](https://2018.igem.org/Team%3ASMS_Shenzhen/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ASMS_Shenzhen/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ASMS_Shenzhen/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ASMS_Shenzhen/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ASMS_Shenzhen/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ASMS_Shenzhen/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ASMS_Shenzhen/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ASMS_Shenzhen/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ASMS_Shenzhen/Hardware)
[Measurement](https://2018.igem.org/Team%3ASMS_Shenzhen/Measurement)
[Model](https://2018.igem.org/Team%3ASMS_Shenzhen/Model)
[Plant](https://2018.igem.org/Team%3ASMS_Shenzhen/Plant)
[Software](https://2018.igem.org/Team%3ASMS_Shenzhen/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=SMS_Shenzhen)

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:SMS Shenzhen/Experiments

Title

* [Home](https://2018.igem.org/Team%3ASMS_Shenzhen)

  [Project](https://2018.igem.org/Team%3ASMS_Shenzhen/Description)

  [Description](https://2018.igem.org/Team%3ASMS_Shenzhen/Description)
  [Design](https://2018.igem.org/Team%3ASMS_Shenzhen/Applied_Design)
  [Demonstrate](https://2018.igem.org/Team%3ASMS_Shenzhen/Demonstrate)

  [Experiments](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments)

  [Experiment Overview](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments)
  [Preliminary Experiments](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments/PriliminaryExperiments)
  [Enzyme Activity](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments/EnzymeActivity)
  [Optic Observation](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments/OpticObservation)
  [Results](https://2018.igem.org/Team%3ASMS_Shenzhen/Results)

  [Parts](https://2018.igem.org/Team%3ASMS_Shenzhen/Parts)

  [Parts](https://2018.igem.org/Team%3ASMS_Shenzhen/Parts)
  [InterLab](https://2018.igem.org/Team%3ASMS_Shenzhen/InterLab)

  [Collaborations](https://2018.igem.org/Team%3ASMS_Shenzhen/Collaborations)

  [Human Practices](https://2018.igem.org/Team%3ASMS_Shenzhen/Human_Practices)

  [Human Practices](https://2018.igem.org/Team%3ASMS_Shenzhen/Human_Practices)
  [Engagement](https://2018.igem.org/Team%3ASMS_Shenzhen/Public_Engagement)

  [Achievement](https://2018.igem.org/Team%3ASMS_Shenzhen/Achievement)

  [Safety](https://2018.igem.org/Team%3ASMS_Shenzhen/Safety)

  [Team](https://2018.igem.org/Team%3ASMS_Shenzhen/Team)

  [Members](https://2018.igem.org/Team%3ASMS_Shenzhen/Team)
  [Attributions](https://2018.igem.org/Team%3ASMS_Shenzhen/Attributions)

Title

![](https://static.igem.org/mediawiki/2018/b/ba/T--SMS_Shenzhen--6.jpg)

# I Overview of Experiment Design Ideas

There are three crucial aspects we can focus on to prove the plausibility of our design concept, including the preliminary characteristic determination of participant sugars, the enzyme activities of the two target enzymes in realistic conditions of oral cavities, and the optic observations about the enzyme effectiveness on real scale teeth.

# II Experiment Design

### Please click on "more details" to view detailed experiment designs and procedures.

#

**Experiment 1: Preliminary Experiments:**

We characterize the stability of reaction participant sugers in various of realistic thermal and pH conditions

[**(Click here for more details)**](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments/PriliminaryExperiments)

#

**Experiment 2: Enzyme Activity Measurements**

We measure the activity of two target enzymes - DexA and FruA

[**(Click here for more details)**](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments/EnzymeActivity)

#

**Experiment 3: Optic Observation of Enzyme Effectiveness on Teeth sample**

We demonstrated how our project will function on real scale teeth by using colour developing agent of dental plaque and image analysis

[**(Click here for more details)**](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments/OpticObservation)

![](https://static.igem.org/mediawiki/2018/5/55/T--SMS_Shenzhen--green.jpg)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:SMS Shenzhen/Results

Title

* [Home](https://2018.igem.org/Team%3ASMS_Shenzhen)

  [Project](https://2018.igem.org/Team%3ASMS_Shenzhen/Description)

  [Description](https://2018.igem.org/Team%3ASMS_Shenzhen/Description)
  [Design](https://2018.igem.org/Team%3ASMS_Shenzhen/Applied_Design)
  [Demonstrate](https://2018.igem.org/Team%3ASMS_Shenzhen/Demonstrate)

  [Experiments](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments)

  [Experiment Overview](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments)
  [Preliminary Experiments](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments/PriliminaryExperiments)
  [Enzyme Activity](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments/EnzymeActivity)
  [Optic Observation](https://2018.igem.org/Team%3ASMS_Shenzhen/Experiments/OpticObservation)
  [Results](https://2018.igem.org/Team%3ASMS_Shenzhen/Results)

  [Parts](https://2018.igem.org/Team%3ASMS_Shenzhen/Parts)

  [Parts](https://2018.igem.org/Team%3ASMS_Shenzhen/Parts)
  [InterLab](https://2018.igem.org/Team%3ASMS_Shenzhen/InterLab)

  [Collaborations](https://2018.igem.org/Team%3ASMS_Shenzhen/Collaborations)

  [Human Practices](https://2018.igem.org/Team%3ASMS_Shenzhen/Human_Practices)

  [Human Practices](https://2018.igem.org/Team%3ASMS_Shenzhen/Human_Practices)
  [Engagement](https://2018.igem.org/Team%3ASMS_Shenzhen/Public_Engagement)

  [Achievement](https://2018.igem.org/Team%3ASMS_Shenzhen/Achievement)

  [Safety](https://2018.igem.org/Team%3ASMS_Shenzhen/Safety)

  [Team](https://2018.igem.org/Team%3ASMS_Shenzhen/Team)

  [Members](https://2018.igem.org/Team%3ASMS_Shenzhen/Team)
  [Attributions](https://2018.igem.org/Team%3ASMS_Shenzhen/Attributions)

Title
![](https://static.igem.org/mediawiki/2018/b/b6/T--SMS_Shenzhen--15.jpg)

# I Glucose Standard Curve

![](https://static.igem.org/mediawiki/2018/5/51/T--SMS_Shenzhen--%E8%91%A1%E8%90%84%E7%B3%96%E6%A0%87%E5%87%86%E6%9B%B2%E7%BA%BF.PNG)

#

![](https://static.igem.org/mediawiki/2018/c/cc/T--SMS_Shenzhen--Shiyan1.png)

#

![](https://static.igem.org/mediawiki/2018/8/8a/T--SMS_Shenzhen--ResultTop.jpg)

#

## Data Analysis:

The experiment 1 illustrates the relationship between the concentration of glucose and the absorbance of the solution with DNS colorant. By using linear regression, we constructed a function with glucose concentration (μmol) as the x-axis and the ab-sorbance as the y-axis.

In the experiment 2, we measured the stability of sucrose and dextran, as well as pH stability. We let the reaction take place in the hot pot set at 100℃. We observed that dextran has a poor hot stability. Moreover, the sucrose will decompose spontaneous-ly under the acidic condition while heat doesn’t affect the decomposition of sucrose.

# II DEX Enzyme Activity

#

![](https://static.igem.org/mediawiki/2018/0/02/T--SMS_Shenzhen--%E9%85%B6%E6%B4%BB%E5%9B%BE%E7%89%87.PNG)

#

## Data Analysis:

From the first half of the graph, we can clearly discover the linear relationship between enzyme concentration and the absorbance. Data suggest that as the concentration of enzyme becomes greater, the relative absorbance increase proportionately. This result justified our assumption. However, the latter half illustrated a negative-related relationship. We suspected that we didn’t use enough substrate to allow the decomposition reaction. Also, we assumed that excessive concentration of enzymes will negatively affect the reaction rate.

![](https://static.igem.org/mediawiki/2018/5/55/T--SMS_Shenzhen--green.jpg)