iGEM 2021 — Team TEC_COSTA_RICA

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:TEC COSTA RICA/Notebook

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2019 Georgia State](https://2019.igem.org/Team%3AGeorgia_State/Notebook)
* [2019 Newcastle](https://2019.igem.org/Team%3ANewcastle/Notebook)
* [2020 IISER Pune India](https://2020.igem.org/Team%3AIISER-Pune-India/Notebook)
* [2020 Lund](https://2020.igem.org/Team%3ALund/Notebook)
* [2020 NOVA LxPortugal](https://2020.igem.org/Team%3ANOVA_LxPortugal/Notebook)
* [2020 RDFZ China](https://2020.igem.org/Team%3ARDFZ-China/NoteBook)

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:TEC COSTA RICA/Engineering

![CronoBacter Logo](https://static.igem.org/mediawiki/2021/5/54/T--TEC_COSTA_RICA--HOME_00_Logo.svg)

* [Home](https://2021.igem.org/Team%3ATEC_COSTA_RICA)
* [Contribution](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Contribution)
* Project
  + [Description](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Description)
  + [Engineering
    Success](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Engineering)
  + [Implementation](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Implementation)
  + ---
  + [Model](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Model)
  + [Measurement](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Measurement)
  + ---
  + [Proof Of Concept](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Proof_Of_Concept)
  + [Software](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Software)
  + [Wet Lab](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Wet_Lab)
  + [Excellence In Another Area](https://2021.igem.org/Team%3ATEC_COSTA_RICA/ExcellenceInAnotherArea)
* Engagement
  + [Collaborations](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Collaborations)
  + [Human Practices](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Human_Practices)
  + [Education](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Education)
  + + [Communication](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Communication)
* [Team](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Attributions)
* [Judging Form](https://2021.igem.org/Team%3ATEC_COSTA_RICA/Medals)

![](https://static.igem.org/mediawiki/2021/f/f8/T--TEC_COSTA_RICA--Header_10.png)

# ENGINEERING FOR SUCCESS

##### In order to successfully implement synthetic biology an engineering framework is necessary. The framework used for our project consisted of four stages: design, build, test, and learn.

During the Design stage the problem and solution are specified. For genetic circuits, the Design is made based on their intended function, construction and characterization; whereas, for modelling and framework, during the Design stage discussion is carried out and the path to follow: models to be built, areas to encompass and their depth. The Build stage of the process includes the construction of circuits, models, the framework itself and other relevant instruments. The constructs, framework and models are assessed during the Test phase. Finally, during the Learn stage the test results are analysed and reflected upon, sometimes behaving as a detonator for a new cycle.

During the Design stage the problem and solution are specified. For genetic circuits, the Design is made based on their intended function, construction and characterization; whereas, for modelling and framework, during the Design stage discussion is carried out and the path to follow: models to be built, areas to encompass and their depth. The Build stage of the process includes the construction of circuits, models, the framework itself and other relevant instruments. The constructs, framework and models are assessed during the Test phase. Finally, during the Learn stage the test results are analysed and reflected upon, sometimes behaving as a detonator for a new cycle.

ODE Model
Gillespie Algorithm
Maximum Population
Promoter Strength
Metabolic Burden

During the Design stage the problem and solution are specified. For genetic circuits, the Design is made based on their intended function, construction and characterization; whereas, for modelling and framework, during the Design stage discussion is carried out and the path to follow: models to be built, areas to encompass and their depth. The Build stage of the process includes the construction of circuits, models, the framework itself and other relevant instruments. The constructs, framework and models are assessed during the Test phase. Finally, during the Learn stage the test results are analysed and reflected upon, sometimes behaving as a detonator for a new cycle.

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:TEC COSTA RICA/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2019 Nantes](https://2019.igem.org/Team%3ANantes/Experiments)
* [2019 TU Eindhoven](https://2019.igem.org/Team%3ATU_Eindhoven/Experiments)
* [2019 Mingdao](https://2019.igem.org/Team%3AMingdao/Demonstrate)
* [2020 Amsterdam](https://2020.igem.org/Team%3AAmsterdam/Experiments)
* [2020 NCTU Formosa](https://2020.igem.org/Team%3ANCTU_Formosa/Experiments)
* [2020 USAFA](https://2020.igem.org/Team%3AUSAFA/Experiments)

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:TEC COSTA RICA/Results

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2019 Newcastle](https://2019.igem.org/Team%3ANewcastle/Results)
* [2019 Munich](https://2019.igem.org/Team%3AMunich/Results)
* [2019 Tec Chihuahua](https://2019.igem.org/Team%3ATec-Chihuahua/Results)
* [2020 Aalto Helsinki](https://2020.igem.org/Team%3AAalto-Helsinki/Results)
* [2020 GreatBay SCIE](https://2020.igem.org/Team%3AGreatBay_SCIE/Results)
* [2020 Queens Canada](https://2020.igem.org/Team%3AQueens_Canada/Results)