iGEM 2022 — Team guelph

{% extends "layout.html" %}
{% block title %}Notebook{% endblock %}
{% block page\_content %}

## iGEM Guelph 2022 Lab Notebook

---

This year iGEM Guelph had been working hard to complete our project. This document offers insight into the different experimental procedures we took to achieve our project as well as the successes and failures that we have encountered and overcome.

{% endblock %}

###### Table 2.Results from the Cytotoixicty Assay

| Raw Numbers | Replicate | *B.subtilis* Empty Backbone | *B.subtilis* Cyt1Aa | *B.subtilis* Cyt2Ba | *E.coli* Cyt1Aa | *E.coli* Cyt2Ba |
| --- | --- | --- | --- | --- | --- | --- |
| # of *D. melanogaster* killed | 1 | 0/3 | 2/3 | 7/7 | 1/8 | 2/5 |
| # of *D. melanogaster* killed | 2 | 0/4 | 1/3 | 4/5 | 2/4 | 3/6 |
| # of *D. melanogaster* killed | 3 | 1/3 | 3/3 | 1/3 | 2/3 | 2/4 |
| Total Killed | - | 1 | 6 | 12 | 5 | 7 |
| Total Tested | - | 10 | 9 | 15 | 15 | 15 |
|
| Percentage Killed (%) | 1 | 0 | 66 | 100 | 12.5 | 40 |
| Percentage Killed (%) | 2 | 0 | 33 | 80 | 50 | 50 |
| Percentage Killed (%) | 3 | 33 | 100 | 33 | 66 | 50 |
| Average Percentage Killed (%) | - | 11 | 66.3 | 71 | 42.8 | 46.7 |

{% extends "layout.html" %}
{% block title %}Experiments{% endblock %}
{% block page\_content %}

## iGEM Guelph 2022 Lab Protocols

---

Here you can find the protocols used by our wet lab team throughout the cloning process. Some protocols have been modified based on experience or from the guidance of our PIs. We hope that this document can be valuable and useful to further iGEM teams.

{% endblock %}

###### Table 2.Results from the Cytotoixicty Assay

| Raw Numbers | Replicate | *B.subtilis* Empty Backbone | *B.subtilis* Cyt1Aa | *B.subtilis* Cyt2Ba | *E.coli* Cyt1Aa | *E.coli* Cyt2Ba |
| --- | --- | --- | --- | --- | --- | --- |
| # of *D. melanogaster killed* | 1 | 0/3 | 2/3 | 7/7 | 1/8 | 2/5 |
| # of *D. melanogaster killed* | 2 | 0/4 | 1/3 | 4/5 | 2/4 | 3/6 |
| # of *D. melanogaster killed* | 3 | 1/3 | 3/3 | 1/3 | 2/3 | 2/4 |
| Total Killed | - | 1 | 6 | 12 | 5 | 7 |
| Total Tested | - | 10 | 9 | 15 | 15 | 15 |
|
| Percentage Killed (%) | 1 | 0 | 66 | 100 | 12.5 | 40 |
| Percentage Killed (%) | 2 | 0 | 33 | 80 | 50 | 50 |
| Percentage Killed (%) | 3 | 33 | 100 | 33 | 66 | 50 |
| Average Percentage Killed (%) | - | 11 | 66.3 | 71 | 42.8 | 46.7 |