iGEM 2017 — Team Grenoble-Alpes

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# Team:Grenoble-Alpes/Notebook

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 Notebook

### Notebook

![](https://static.igem.org/mediawiki/2017/c/c9/LogolocalisationiGEMGrenoble.png)
 This highliner seems to fly over Grenoble’s city. The photograph was taken on the heights of Lans en Vercors, during the “Marmotte Highline Project”, sweet souvenirs.
 Credits: Estelle Vincent

##### [Science Fair](#key1) ...is a French national event... [NRJ Interview](#key2) ...was contacted by Cyril... [“UGA C’est Party”](#key3) ...new tradition of our university... [“Apéritif dinatoire”](#key4) ... a better way to thank everyone... [Photoshoot session](#key5) ...beautiful day for... [Popular Scientific Lectures](#key6) ...make science accessible to everyone... [Genetically Modified Music](#key7) ...as our very first outreach event...

Science Fair

![](https://static.igem.org/mediawiki/2017/4/4c/GrenobleAlpessciencefair.png )

##### October 12-14th, 2017 Created in 1992, the Science Fair is a French national event of scientific popularization. 2017’s topic was “How can we change the preconceived ideas in science ?”. We had the chance to have our own stand to present the iGEM competition as well as our project SnapLab. Our main aim was to popularize synthetic biology and the field in which it is applied.

[##### MORE](#article1)

NRJ Interview

![](https://static.igem.org/mediawiki/2017/2/2b/GrenobleAlpesnrj.png )

##### September 26th, 2017 Our team was contacted by Cyril, a journalist working for the world class radio “NRJ”. On September the 26th, our team was welcomed at NRJ’s studio.

[##### MORE](#article2)

[×](#articles1-2)

![](https://static.igem.org/mediawiki/2017/4/4c/GrenobleAlpessciencefair.png)

Created in 1992, the Science Fair is a French national event of scientific popularization. 2017’s topic was “How can we change the preconceived ideas in science ?”.
We had the chance to have our own stand to present the iGEM competition as well as our project SnapLab. Our main aim was to popularize synthetic biology and the field in which it is applied.
We met a lot of different profiles: adults and kids, with or without scientific backgrounds, with or without knowledge in biology… This is why we had to adapt our explanations depending on the type of public we were facing. This allowed us to tremendously improve our scientific communication skills ! As we had the visit of classrooms, from middle and high school level, we also organized quizzes about the basics in biology: “What do you know about DNA ?”, “What is a genetically modified organism ?”.
The experience was very enriching and receiving the enthusiasm of the public was extremely gratifying. We were even interviewed and got an article about iGEM project in a newspaper!

[×](#articles1-2)

![](https://static.igem.org/mediawiki/2017/2/2b/GrenobleAlpesnrj.png )

Our team was contacted by Cyril, a journalist working for the world class radio “NRJ”. On September the 26th, our team was welcomed at NRJ’s studio. We started by introducing ourselves as well as the iGEM contest and the applications of synthetic biology. Cyril also asked for a little bit more informations on our project, from the administrative part to the fundraising campaign and the scientific realisation of our kit: SnapLab.

“UGA C’est Party”, a new tradition

![](https://static.igem.org/mediawiki/2017/a/a0/GrenobleAlpesUGAcestparty.png)

##### September 14th, 2017 This is the new tradition of our university: once a year in September, all students are welcome to the central place of the campus. Many different animations, associations and activities are proposed to the students for free. For the very first time, iGEM Grenoble-Alpes was represented during this event.

[##### MORE](#article3)

Because we detected fluorescence!

![](https://static.igem.org/mediawiki/2017/7/70/GrenobleAlpesdetection.jpeg )

##### July 25th, 2017 Before being able to detect fluorescence that bacteria are going to emit, we have to make sure our system is capable of detecting fluorescence! And, that’s what we did! First time we detected fluorescence, we are on the right direction but still lots of work to achieve!

[##### MORE](#article4)

[×](#articles3-4)

![](https://static.igem.org/mediawiki/2017/a/a0/GrenobleAlpesUGAcestparty.png )

This is the new tradition of our university: once a year in September, all students are welcome to the central place of the campus. Many different animations, associations and activities are proposed to the students for free. For the very first time, iGEM Grenoble-Alpes was represented during this event. Despite the bad weather, we met several people interested in SnapLab and low-cost diagnostic kits. At the end of the day, a giant concert took place.
We would like to thank the organizers as well as all the people he talked to.

[×](#articles3-4)

![](https://static.igem.org/mediawiki/2017/7/70/GrenobleAlpesdetection.jpeg )

Before being able to detect fluorescence that bacteria are going to emit, we have to make sure our system is capable of detecting fluorescence! And, that’s what we did! First time we detected fluorescence, we are on the right direction but still lots of work to achieve!

“Apéritif dinatoire”

![](https://static.igem.org/mediawiki/2017/c/cd/GrenobleAlpeschalet.jpeg)

##### July 20st, 2017 What a better way to thank everyone that supported us so far than organizing an “Apéritif Dinatoire” as we would say in French.

[##### MORE](#article5)

Photoshoot session

![](https://static.igem.org/mediawiki/2017/7/72/GrenobleAlpesphotoshoot.jpeg)

##### July 13rd, 2017 Such a beautiful day for a photoshoot session! So as to present ourselves on our website, we had this idea to take photos in a street art environment. Grenoble hosts each year the Street Art festival, giving us the opportunity to discover amazing piece of arts as well as artists. In the depths of Grenoble’s streets, fun we had!

[##### MORE](#article6)

[×](#articles5-6)

![](https://static.igem.org/mediawiki/2017/c/cd/GrenobleAlpeschalet.jpeg)

What a better way to thank everyone that supported us so far than organizing an “Apéritif Dinatoire” as we would say in French.

[×](#articles5-6)

![](https://static.igem.org/mediawiki/2017/7/72/GrenobleAlpesphotoshoot.jpeg)

Such a beautiful day for a photoshoot session! So as to present ourselves on our website, we had this idea to take photos in a street art environment. Grenoble hosts each year the Street Art festival, giving us the opportunity to discover amazing piece of arts as well as artists.
In the depths of Grenoble’s streets, fun we had!

First success with electronics

![](https://static.igem.org/mediawiki/2017/c/c3/GrenobleAlpestempcontrol.jpeg)

##### June 16th, 2017 After struggling with electronics vibes for a while, we were first able to make our electronic circuit work. A first step in making our temperature control system to work…

[##### MORE](#article7)

Motion design, animation serving communication

![](https://static.igem.org/mediawiki/2017/8/86/Miniature_video_presentation_igem_grenoble_2017.png)

##### June 5th, 2017 Motion design is a graphic art design consisting of creating animated objects delivering a message in a fun way. It has to be dynamic, fast, creative and attractive. In this spirit, we realised a short motion design movie for our crowdfunding campaign.

[##### MORE](#article8)

[×](#articles7-8)

![](https://static.igem.org/mediawiki/2017/c/c3/GrenobleAlpestempcontrol.jpeg)

After struggling with electronics vibes for a while, we were first able to make our electronic circuit work. A first step in making our temperature control system to work…

[×](#articles7-8)

[![
Your browner cannot show this video.
](https://static.igem.org/mediawiki/2017/8/86/Miniature_video_presentation_igem_grenoble_2017.png)](https://static.igem.org/mediawiki/2017/c/cd/Video_presentation_iGEM_Grenoble_2017.mp4)

Motion design is a graphic art design consisting of creating animated objects delivering a message in a fun way. It has to be dynamic, fast, creative and attractive. In this spirit, we realised a short motion design movie for our crowdfunding campaign.
The scenario was divided into 4 parts, each of them represented by one colour, with flat designed objects. This is the final result, we apologize for it being only in French.

Thinking, thinking and solving the DNA Extraction

 Kit’s issues

![](https://static.igem.org/mediawiki/2017/8/89/GrenobleAlpescolonne.png)

##### June 4th, 2017 In laboratory, DNA is usually extracted by the means of different types of kit. The most famous are the « Mini preps » kits and they use a centrifuge. But, to respect our portability criterion we had to find a way to extract DNA without a centrifuge! How ? That is the question.

[##### MORE](#article9)

“La Casemate” Fab Lab, our place to

 “make almost everything”

![](https://static.igem.org/mediawiki/2017/c/cb/GrenobleAlpesfablab.jpeg)

##### May 6th, 2017 FabLabs are collaborative workspaces that welcome people from any age or field to help them carry out their projects. They are an alternative to mass-production, giving people access to computer-controlled tools that are usually restricted to industrial uses such as 3D printer, laser cutter or vinyl cutter. Fab Labs have thus embodied the “Do It Yourself” approach, encouraging the use of free and open source software.

[##### MORE](#article10)

[×](#articles9-10)

![](https://static.igem.org/mediawiki/2017/8/89/GrenobleAlpescolonne.png)

In laboratory, DNA is usually extracted by the means of different types of kit. The most famous are the « Mini preps » kits and they use a centrifuge. But, to respect our portability criterion we had to find a way to extract DNA without a centrifuge! How ? That is the question.
After many hours of thinking and putting ourselves under pressure, it was indeed pressure that was the answer to our problems. Pressure can force a liquid to pass from one compartment to another but again the question was now! How does pressure need to be applied in the extraction column? Well, the idea would be to use a needle of the same diameter as the column’s and replace every step of centrifuging by the pressure using the needle. In the end, we planned to obtain a good genomic DNA concentration that would permit the diagnosis! You can look at our results for more informations!

[×](#articles9-10)

![](https://static.igem.org/mediawiki/2017/c/cb/GrenobleAlpesfablab.jpeg )

FabLabs are collaborative workspaces that welcome people from any age or field to help them carry out their projects. They are an alternative to mass-production, giving people access to computer-controlled tools that are usually restricted to industrial uses such as 3D printer, laser cutter or vinyl cutter. Fab Labs have thus embodied the “Do It Yourself” approach, encouraging the use of free and open source software.
We had the chance to work in collaboration with the Fab Lab “La Casemate” in Grenoble for our iGEM project. We had a great time sharing with the other Makers on various projects, as well as on our own with which they were very helpful throughout the different issues we faced, so thanks a lot!

Popular Scientific Lectures

![](https://static.igem.org/mediawiki/2017/5/54/GrenobleAlpescafescience.png)

##### 27th April, 2017 One of the demands of the iGEM contest, which we have at heart, is to make science accessible to everyone by the popularization of science. To this end, a popular scientific conference was organized. On this “Café Science” evening, we received 3 speakers from 3 different scientific fields.

[##### MORE](#article11)

3D printing, a promising technique

![](https://static.igem.org/mediawiki/2017/b/b7/GrenobleAlpes3Dprinting1.jpeg)

##### April 7th, 2017 In the recent years, 3D-printing has become a fast and simple way to implement any kinds of ideas. As this technology is compatible with many different domains, such as food industry, pharmaceutical field, biotechnology, 3D-printing has a fast unlimited growing potential. As a matter of fact, in cell therapy, new innovative treatments have been brought by 3D printing, like the creation of blood vessels from stem cells.

[##### MORE](#article12)

[×](#articles11-12)

![](https://static.igem.org/mediawiki/2017/5/54/GrenobleAlpescafescience.png)

One of the demands of the iGEM contest, which we have at heart, is to make science accessible to everyone by the popularization of science. To this end, a popular scientific conference was organized. On this “Café Science” evening, we received 3 speakers from 3 different scientific fields. Yahia Chebloune, director of PAVAL Lab in INRA Grenoble (top right photo) talked about his latest amazing progresses in the design of an HIV vaccine. Then, Eduard Ayuso, INSERM researcher from University of Nantes (bottom left photo) gave a wonderful lecture about viral vectors and gene therapy. Finally, François Rousse, PhD student in INRIA Grenoble (bottom right photo), was the last speaker with a presentation about biomimetics in mathematics, i.e. the way mathematicians try to mimic natural biological processes. At the end of each talk, the public had the opportunity to ask any kinds of questions or/and start a discussion with the researcher. Around 50 people attended this conference; the public was mainly composed of students -bachelor and master’s degree- but also teachers, speakers’ colleagues, and even students’ relatives. This nice evening of exchange ended with a buffet offered by our team.

[×](#articles11-12)

![](https://static.igem.org/mediawiki/2017/b/b7/GrenobleAlpes3Dprinting1.jpeg)![](https://static.igem.org/mediawiki/2017/3/3b/GrenobleAlpes3Dprinting2.jpeg)

In the recent years, 3D-printing has become a fast and simple way to implement any kinds of ideas. As this technology is compatible with many different domains, such as food industry, pharmaceutical field, biotechnology, 3D-printing has a fast unlimited growing potential. As a matter of fact, in cell therapy, new innovative treatments have been brought by 3D printing, like the creation of blood vessels from stem cells. Completely impressed by what could be achieved with 3D printing technique, we came down back to earth and focused on how we could concretize our kit. Well, 3D printing is obviously the answer! This limitless technique was the solution to create SnapLab, and even though it sounds simple we still had to optimize the process. But, we cannot deny this feeling of satisfaction when the first pieces of our kit was 3D printed ! Just remember that 3D printing is for sure a technique opening a wide new range of possibilities that we definitely must take advantage of.

Genetically Modified Music

![](https://static.igem.org/mediawiki/2017/7/78/GrenobleAlpesconcert.png )

##### November 3rd, 2016 We decided to organize a concert as our very first outreach event. EVE was the most appropriate place in Grenoble because this place was built for students. By making it affordable to everyone, this cultural event was an undeniable success. Everyone had fun and were positively surprised by the variety of music genres, from Jazz to Death Metal. We would like to thank each group that participated in this concert.

[##### MORE](#article13)

We are a team now !

![](https://static.igem.org/mediawiki/2017/a/a9/RemercimentEquipe.png)

##### October 25th, 2017 After a teacher mentioned iGEM in two different classes, iGEM Grenoble team started. A few simple words raised a strong motivation! Weeks later, a meeting was organized between teachers of the School of Pharmacy (who first wanted to start a new team); teachers of Phelma Engineering School (who supervised the previous iGEM teams in 2012 and 2013) ; and 3 students in Biotechnology who expressed their interests in the project.

[##### MORE](#article14)

[×](#articles13-14)

![](https://static.igem.org/mediawiki/2017/7/78/GrenobleAlpesconcert.png)

We decided to organize a concert as our very first outreach event. EVE was the most appropriate place in Grenoble because this place was built for students. By making it affordable to everyone, this cultural event was an undeniable success. Everyone had fun and were positively surprised by the variety of music genres, from Jazz to Death Metal. We would like to thank each group that participated in this concert.
Organic Groonje played some efficient Jazz and happy music, which was the perfect warmup to wake up the crowd! Anomaly ‘s performance was incredible, which we will definitely not forget. Norwalk has confirmed all the good words after the contest Headbang that they’ve won. Plague From The Void played their new set, they put EVE on fire and gave us a great help before the concert. Finally, Crawl To The Past finished perfectly the night, they even broke their wands to fed this crowd.

[×](#articles13-14)

![](https://static.igem.org/mediawiki/2017/a/a9/RemercimentEquipe.png)

After a teacher mentioned iGEM in two different classes, iGEM Grenoble team started.
A few simple words raised a strong motivation! Weeks later, a meeting was organized between teachers of the School of Pharmacy (who first wanted to start a new team); teachers of Phelma Engineering School (who supervised the previous iGEM teams in 2012 and 2013) ; and 3 students in Biotechnology who expressed their interests in the project. From that day, a call for applications started to gather every Biotechnology students that was interested in iGEM. We contacted the 2013 participants so they could give us information, advices, and also… the association, created in 2011 by the very first iGEM Grenoble team. The main aim of this association was to ease the finances. In parallel, we met a few times for brainstormings, to define a project among the tremendous number of ideas we accumulated. It appeared very quickly that we would need students from other fields with other skills, notably in engineering. So, one of us went to present the contest at the beginning of a class of biomedical engineering students, at Phelma. Candidacies arrived within the week and we all met to improve our brainstorming. We particularly learned to know each other the night of our first event: the concert. Soon after, our project was chosen unanimously ! Other recruiting processes were organised in different education sectors but students did not show as enthusiastic as the engineers. During the fundraising period, some students left the adventure. After a few months, we presented the project to designer students, who showed high motivation to help us to present SnapLab. One year later here we are, a smiling team of 12 students: 6 studying Biotechnology/Biology, 5 studying Biomedical Engineering and 2 in 3D modelling/VFX !

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# Team:Grenoble-Alpes/Engineering

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 Engineering

### Detection

![](https://static.igem.org/mediawiki/2017/c/c9/LogolocalisationiGEMGrenoble.png)
 It was at Amont à Aussoi that we had met that night to sleep by the fireside.
 Credits: Estelle Vincent

### How to capture fluorescence ?

##### The first step was to look into existing ways of capturing fluorescence. Photodiodes are commonly used since they convert light into an electrical current, which is produced when photons are absorbed in the photodiode. As surprising as it can be, smartphones are now widely used in medical diagnosis as smartphones’ cameras have the same accuracy as any photodiodes. Plus, as smartphones are part of our daily lives, not only they are an effective solution but a very practical one as well, especially in remote places like Africa. The theory behind the implementation of this technique is as follows. A light filtered by an excitation filter is shed on the object which is positioned perpendicularly to the optical axis. A fluorescent light is back emitted by the object and then filtered by an emission filter. This light is ultimately collected using an optical zoom sticked to the smartphone’s camera.

![](https://static.igem.org/mediawiki/2017/d/d7/Igemgrenoble_detection_schemaglobal.png)

[![](https://static.igem.org/mediawiki/2017/e/e1/Igemgrenoble_detection_filters.png)](#ancre_filters")

[![](https://static.igem.org/mediawiki/2017/5/50/Igemgrenoble_detection_emissionlight.png)](#ancre_emission)

[![](https://static.igem.org/mediawiki/2017/8/8e/Igemgrenoble_detection_opticaldevices.png)](#ancre_optical)

[![](https://static.igem.org/mediawiki/2017/8/88/Igemgrenoble_detection_lightintensity.png)](#ancre_light)

## Filters

##### The fluorophore wavelength has to be known as precisely as possible so that the appropriate filters can then be acquired. [SpectraViewer](https://www.thermofisher.com/ch/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html) is a useful tool to simulate the spectral responsivity. Here, the spectral responsivity of the Red Fluorescent Protein (RFP) is simulated.

![](https://static.igem.org/mediawiki/2017/e/e8/Igemgrenoble_detection_spectraviewer1.png)

##### RFP has a maximum excitation at 555 nm and the maximal emission wavelength is at 584 nm. Therefore, our goal is to excite the fluorophore using a filter centered as close as possible to 558 nm without however having the excitation spectrum overlap the emission spectrum. To achieve this, a bandpass filter has been used for the excitation spectrum, centered on 546 nm. As for the emission filter, it consists of a low pass filter cutting high frequencies. That way the two spectra are clearly distinct, allowing a high precision measurement.

## Emission light

##### For this parameter two options have been considered, either a white lamp or LED. The LED had the advantage of having a very narrow bandwidth and therefore did not require any filter. However, LEDs are low power light sources, which is problematic because a lower power implied a lesser precision in the measurement. For this reason, using a more powerful white lamp combined with the appropriate filter has been prefered.

## Optical devices

##### First and foremost, it is important to note that the excitation light is positioned perpendicularly to the smartphone, as this reduces light scattering. Our optical system is organized as follows: the lamp to which we adjoin the excitation filter, followed by the object and ultimately the adjoined emission filter, lens and smartphone camera.

##### It goes without saying that since the goal is to detect emitted light, it is of the utmost importance to work in an environment as dark as possible so as to avoid any straylight. Ultimately, as the smartphone’s camera is a little too close from the sample, a lense has been added prior to the camera. This provides us with two big advantages: the image occupies the sensor of the camera, that way more emitted light can be detected, and also the image is sharper, thus taking full advantage of the camera’s quality.

## Light intensity

##### Once a picture has been taken with a smartphone, it needs to be analyzed to provide a numerical value that will help to quantify the amount of fluorescence present. Fluorescence is a value with no unit, it has no reference value which is the reason why a blank must be done serving as the reference value. Therefore, there is no universal way to quantify it and each device could give different values to what is being measured. The goal here is to be able to derive a number from the picture which could inform us on the brightness of the picture, which could in turn provide us information on the amount of fluorescence.

##### To do so, the picture is analysed using a program on Matlab. The picture is first set to black and white, on a classic 256 gray scale, where 0 is for a black pixel and 255 for the white ones. Then a sum of all the values is done, thus returning a single value. The higher this value is the better, because it would mean that there is more fluorescence overall. As the picture is taken in a dark environment, if a picture appears bright enough it can be due to the fluorescence. It is important to note that for each picture the frame is the same, the tubes are placed at the same place implying that the surface captured each time is the same. We can therefore evaluate the amount of fluorescence by applying the calculation to the entire picture and compare the values obtained. The numerical value obtained can then be compared to one obtained using a measurement instrument, such as a spectrophotometer, and conclude if the method and the overall system are reliable.

### TESTS REALISED

##### The goal of the test is to verify that the detection of fluorescence is occurring as expected and determine if there is a certain threshold under which fluorescence cannot be detected anymore.

## Protocol and implementation

##### - 6 test tubes are prepared, each having the same volume and optical density (OD) so as their fluorescence can be compared.

##### - Fluorescent bacteria are used as well as non-fluorescent. In fact, the OD of each tube has to be the same since the less there are fluorescent bacteria, the smaller the value of OD is, that’s why it is compensated by adding nonfluorescent bacteria. LB is used to compensate the volume, so each tube has the same volume, otherwise a comparison wouldn’t be possible.

##### - The tube’s composition is as follows.

Table 1 : Tube's composition (volumes in mL)

|  | 1 | 2 | 3 | 4 | 5 | 6 |
| Targeted plasmid | 0.00 | 0.02 | 0.04 | 0.06 | 0.08 | 0.1 |
| Non fluorescent bacteria JM109 | 0.50 | 0.419 | 0.338 | 0.257 | 0.176 | 0.094 |
| LB | 0 | 0.061 | 0.122 | 0.183 | 0.244 | 0.305 |

##### - A picture of each tube is then taken individually, with the 3D printed kit. Notice that a cover is put above the kit to create a dark environment.

![](https://static.igem.org/mediawiki/2017/2/26/Igemgrenoble_detection_photokit2.jpeg)

##### - The pictures are then analysed using the Matlab program, which informs us of the intensity of fluorescence detected on the picture.

##### - So as to verify that our device is working properly, measurements are made at the same time with a spectrophotometer, allowing for a comparison with the result obtained with the smartphone.

## Results

##### Before making any other comment, it is important to emphasize on the fact that since the same OD were obtained in each tube, it is reasonable to compare the results.

Table 2 : Comparison of optical densities' rate in the different tubes

| Tubes | LB | 1 | 2 | 3 | 4 | 5 | 6 |
| Optical Density | 0 | 0.375 | 0.270 | 0.265 | 0.270 | 0.260 | 0.290 |

##### What is particularly interesting in these results is that we have the possibility to compare them with those of a calibrated spectrophotometer. As shown in the graphs below, both of the measurement devices have a linear variation between the quantity of fluorescent bacteria and the level of fluorescence measured. In fact, we use a smartphone with a camera that contains a light sensor proportional to the light intensity. That being said, it enables us to compare these two devices. It can be seen on the graphs that in both cases, the fainter the fluorescence, the greater the incertitude associated. At this point, there is no threshold, because even though the incertitudes are greater for smaller values, they are of lower magnitude compared to incertitudes of greater values. Thus we can take into account every fluorescence measured.

![](https://static.igem.org/mediawiki/2017/a/a9/Igemgrenoble_detection_spectro.png)

![](https://static.igem.org/mediawiki/2017/6/65/Igemgrenoble_detection_telephone.png)

## Discussion

##### Once the fluorescence detector built appears as an accurate device, it is useful to compare its returned values with the ones returned by the spectrophotometer in a more precise manner. Measurements of fluorescence of the six tubes taken by the spectrophotometer is plot in function of the measurements taken with the smartphone. As it can be seen, the values obtained using the kit vary in the same way as those obtained with the spectrophotometer. As it is safe to assume that the spectrophotometer is returning sensible data, the experimental data can also be expected to be correct.

![](https://static.igem.org/mediawiki/2017/c/c9/Igemgrenoble_detection_telephoneVSspectro.png)

##### What would be interesting here is to calibrate our device, in order to be able to adjust any other smartphones to this kit. The calibration of a device stays an important part of its fabrication and it is important to consider whether the measurement is reproducible or not. Even if that is not our case, we wanted to find a way to calibrate every SnapLab devices. For that purpose, a small sheet of red paper can be delivered with the kit, thus allowing each user to properly calibrate the kit independently of the smartphone they are using. The first step would be to take a picture of this red colored paper with the smartphone used for the analysis. The application could then be able to adjust the sum of the pixels’ intensities to the one given by the device we built with our smartphone.

##### It is worth mentioning that results can only be considered as correct if they are located within the errorbars. The uncertainties have been evaluated as follows: three pictures of the same test tube were taken, over the entire tube tests, that way we can determine if the uncertainty depends on the intensity of the fluorescence or not, just as it would for a spectrophotometer. We then evaluated the standard deviation, and depending on the results, a specific errorbar is given or a single errorbar is considered for the entire spectrum by taking the mean of all the standard deviations. Same work was made for the spectrophotometer in order to compare the results.

Table 3 : Comparison of the reproducibility rates

|  | 1 | Non fluorescent tube | Fluorescent tube |
| Human reproducibility | 91.63% | 90.11% | 95.76% |
| Spectrophotometer reproducibility | 91.64% | 90.80% | 97.79% |
| SnapLab reproducibility | 99.54% | 99.84% | 99.97% |

##### This allows us to comment on the precision of the smartphone’s camera. Concerning the level of fluorescence in each tubes, the more fluorescent it is, the more precise the measurement will be. However, taking into account a minimum threshold is not necessary because measurements stay accurate even if there are less precise. Concerning the values of uncertainties obtained with the smartphone, it appears to be very small. In fact, unlike the uncertainties of the spectrophotometer which are bigger, the experiment made to determine error bars for the smartphone was quite restrictive. Only a few uncertainties were considered, leading to a huge level of reproducibility. To improve this value, other tests could be done considering other sources of uncertainties. It would lower the level of reproducibility but not drastically.

##### Finally, as it can be seen, it can be assumed that the telephone inside the kit is a real measurement tool.

[Back to our general results](https://2017.igem.org/Team%3AGrenoble-Alpes/Demonstrate)

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Designed by iGEM Grenoble-Alpes 2017 team | [Site Map](https://2017.igem.org/Team%3AGrenoble-Alpes/Site_Map)

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# Team:Grenoble-Alpes/Experiments

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MENU

[HOME](https://2017.igem.org/Team%3AGrenoble-Alpes)

TEAM

[Team](https://2017.igem.org/Team%3AGrenoble-Alpes/Team)
[Collaborations](https://2017.igem.org/Team%3AGrenoble-Alpes/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3AGrenoble-Alpes/Description)
[Design](https://2017.igem.org/Team%3AGrenoble-Alpes/Design)
[Experiments](https://2017.igem.org/Team%3AGrenoble-Alpes/Experiments)
[Notebook](https://2017.igem.org/Team%3AGrenoble-Alpes/Notebook)
[InterLab](https://2017.igem.org/Team%3AGrenoble-Alpes/InterLab)
[Contribution](https://2017.igem.org/Team%3AGrenoble-Alpes/Contribution)
[Model](https://2017.igem.org/Team%3AGrenoble-Alpes/Model)
[Results](https://2017.igem.org/Team%3AGrenoble-Alpes/Results)
[Demonstrate](https://2017.igem.org/Team%3AGrenoble-Alpes/Demonstrate)
[Improve](https://2017.igem.org/Team%3AGrenoble-Alpes/Improve)
[Attributions](https://2017.igem.org/Team%3AGrenoble-Alpes/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3AGrenoble-Alpes/Parts)
[Basic Parts](https://2017.igem.org/Team%3AGrenoble-Alpes/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3AGrenoble-Alpes/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3AGrenoble-Alpes/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3AGrenoble-Alpes/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3AGrenoble-Alpes/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3AGrenoble-Alpes/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3AGrenoble-Alpes/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3AGrenoble-Alpes/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3AGrenoble-Alpes/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3AGrenoble-Alpes/Hardware)
[Measurement](https://2017.igem.org/Team%3AGrenoble-Alpes/Measurement)
[Model](https://2017.igem.org/Team%3AGrenoble-Alpes/Model)
[Plant](https://2017.igem.org/Team%3AGrenoble-Alpes/Plant)
[Software](https://2017.igem.org/Team%3AGrenoble-Alpes/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=Grenoble-Alpes)

# Grenoble-Alpes

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

##### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

##### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

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[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:Grenoble-Alpes/Results

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MENU

[HOME](https://2017.igem.org/Team%3AGrenoble-Alpes)

TEAM

[Team](https://2017.igem.org/Team%3AGrenoble-Alpes/Team)
[Collaborations](https://2017.igem.org/Team%3AGrenoble-Alpes/Collaborations)

PROJECT

[Description](https://2017.igem.org/Team%3AGrenoble-Alpes/Description)
[Design](https://2017.igem.org/Team%3AGrenoble-Alpes/Design)
[Experiments](https://2017.igem.org/Team%3AGrenoble-Alpes/Experiments)
[Notebook](https://2017.igem.org/Team%3AGrenoble-Alpes/Notebook)
[InterLab](https://2017.igem.org/Team%3AGrenoble-Alpes/InterLab)
[Contribution](https://2017.igem.org/Team%3AGrenoble-Alpes/Contribution)
[Model](https://2017.igem.org/Team%3AGrenoble-Alpes/Model)
[Results](https://2017.igem.org/Team%3AGrenoble-Alpes/Results)
[Demonstrate](https://2017.igem.org/Team%3AGrenoble-Alpes/Demonstrate)
[Improve](https://2017.igem.org/Team%3AGrenoble-Alpes/Improve)
[Attributions](https://2017.igem.org/Team%3AGrenoble-Alpes/Attributions)

PARTS

[Parts](https://2017.igem.org/Team%3AGrenoble-Alpes/Parts)
[Basic Parts](https://2017.igem.org/Team%3AGrenoble-Alpes/Basic_Part)
[Composite Parts](https://2017.igem.org/Team%3AGrenoble-Alpes/Composite_Part)
[Part Collection](https://2017.igem.org/Team%3AGrenoble-Alpes/Part_Collection)

[SAFETY](https://2017.igem.org/Team%3AGrenoble-Alpes/Safety)

HUMAN PRACTICES

[Silver HP](https://2017.igem.org/Team%3AGrenoble-Alpes/HP/Silver)
[Integrated and Gold](https://2017.igem.org/Team%3AGrenoble-Alpes/HP/Gold_Integrated)
[Public Engagement](https://2017.igem.org/Team%3AGrenoble-Alpes/Engagement)

AWARDS

[Applied Design](https://2017.igem.org/Team%3AGrenoble-Alpes/Applied_Design)
[Entrepreneurship](https://2017.igem.org/Team%3AGrenoble-Alpes/Entrepreneurship)
[Hardware](https://2017.igem.org/Team%3AGrenoble-Alpes/Hardware)
[Measurement](https://2017.igem.org/Team%3AGrenoble-Alpes/Measurement)
[Model](https://2017.igem.org/Team%3AGrenoble-Alpes/Model)
[Plant](https://2017.igem.org/Team%3AGrenoble-Alpes/Plant)
[Software](https://2017.igem.org/Team%3AGrenoble-Alpes/Software)

[JUDGING FORM](https://igem.org/2017_Judging_Form?team=Grenoble-Alpes)

# Grenoble-Alpes

# Results

Here you can describe the results of your project and your future plans.

##### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

##### You should also describe what your results mean:

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

##### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

##### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)

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