iGEM 2015 — Team Harvard_BioDesign

# Team:Harvard BioDesign/Notebook

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[![Harvard Logo](https://static.igem.org/mediawiki/2015/9/94/HarvardBioDesign2015Logo2.png)](https://2015.igem.org/Team%3AHarvard_BioDesign)

#### Main Pages

* [Home](https://2015.igem.org/Team%3AHarvard_BioDesign)
* [Project](https://2015.igem.org/Team%3AHarvard_BioDesign/Project)
* [Results](https://2015.igem.org/Team%3AHarvard_BioDesign/Results)
* [Notebook](https://2015.igem.org/Team%3AHarvard_BioDesign/Notebook)
* [Parts](https://2015.igem.org/Team%3AHarvard_BioDesign/Parts)
* [Practices](https://2015.igem.org/Team%3AHarvard_BioDesign/Practices)
* [Safety](https://2015.igem.org/Team%3AHarvard_BioDesign/Safety)
* [Collaborations](https://2015.igem.org/Team%3AHarvard_BioDesign/Collaborations)
* [Team](https://2015.igem.org/Team%3AHarvard_BioDesign/Team)
* [Attributions](https://2015.igem.org/Team%3AHarvard_BioDesign/Attributions)

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![](https://static.igem.org/mediawiki/2015/5/53/Harvard2015_Notebook.png)

![](https://static.igem.org/mediawiki/2015/f/f8/Harvard_Notebook_1.jpg)

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 6/8

Settle on bacterial adhesion.

Further background research.

Plasmid design, primer design.

Chose fusion peptides.

Ordered gBlocks from IDT of Rhamnose, Lac, and Arabinose promoters.

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 6/15

PCR backbones and fim proteins.

Poured Chloramphenicol and Ampicillin plates.

Gibson assembly of operon plasmid and fimH plasmid.

Pili purification assay development.

NEGEM Meetup #1.

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 6/22

Redo failed PCRs.

Redo failed Gibsons.

Transform and plate operon Gibson.

Pili purification and agglutination assay development.

Microscopy on control strains.

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 6/29

Miniprep operon plasmid.

Sent for sequencing.

Unsuccessful- redo operon PCRs.

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 7/6

Induction.

Operon Gibson.

Agglutination assay development.

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 7/13

Induction.

Edit out illegal PstI cut site from fimH.

Mutagenesis to add binding peptides.

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 7/20

Induction.

Transform measurement study parts.

Mutagenesis to add binding peptides.

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 7/27

Induction.

Nickel binding assay.

Made biobricks for pRha-fimH and fimH. PCR-ing out from parts we were using with low-copy backbone, Gibson with pSB1C3.

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 8/2

Induction. Induction. More Induction.

Nickel binding assay.

Stainless steel binding assay.

Cancer binding assay.

Made biobricks, sent for sequencing.

![](https://static.igem.org/mediawiki/2015/e/e7/Harvard_Notebook_2.jpg)

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week 8/9

Induction. Induction. More Induction.
Nickel binding assay.
Stainless steel binding assay.
Cancer binding assay.
Retried failed biobricks.

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 8/16

Break from lab.

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 8/23

Break from lab.

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 8/30

Wiki and presentation planning.

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 9/6

Retried failed biobricks.
Obtained results for Rhamnose-GFP inductions.
Made presentation.
NEGEM Meetup #2!

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## Week of 9/13

Worked on parts pages.
Worked on wiki.

![](https://static.igem.org/mediawiki/2015/4/43/Harvard2015Cog.png)

## 9/24 - 9/28

iGEM Giant Jamboree!!!

![](https://static.igem.org/mediawiki/2015/7/79/Harvard_Notebook_3.jpg)

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