iGEM 2020 — Team Shanghai_United

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:Shanghai United/Notebook

非模块化方式使用layui

Entprenuership

![](https://static.igem.org/mediawiki/2020/b/b4/T--Shanghai_United--logo.jpg)

**The Filters**

---

* [Home](https://2020.igem.org/Team%3AShanghai_United)
* Project

  :   [Description](https://2020.igem.org/Team%3AShanghai_United/Description)
  :   [Design](https://2020.igem.org/Team%3AShanghai_United/Design)
  :   [Experiments](https://2020.igem.org/Team%3AShanghai_United/Experiment)
  :   [Notebook](https://2020.igem.org/Team%3AShanghai_United/Notebook)
  :   [Result](https://2020.igem.org/Team%3AShanghai_United/Result)
  :   [Safety](https://2020.igem.org/Team%3AShanghai_United/Safety)
* [Contribution](https://2020.igem.org/Team%3AShanghai_United/Contribution)
* [Engineering](https://2020.igem.org/Team%3AShanghai_United/Engineering)
* [Implementation](https://2020.igem.org/Team%3AShanghai_United/Implementation)
* Parts

  :   [Basic Parts](https://2020.igem.org/Team%3AShanghai_United/Basic%20Parts)
  :   [Composite Parts](https://2020.igem.org/Team%3AShanghai_United/Composite%20Parts)
  :   [Parts Collection](https://2020.igem.org/Team%3AShanghai_United/Parts%20Collection)
* [Model](https://2020.igem.org/Team%3AShanghai_United/Model)
* Human Practice

  :   [Integrated Human Practice](https://2020.igem.org/Team%3AShanghai_United/Human_Practices)
  :   [Fundraising](https://2020.igem.org/Team%3AShanghai_United/Fundraising)
  :   [Entrepreneurship](https://2020.igem.org/Team%3AShanghai_United/Entrepreneurship)
* Team

  :   [Team Member](https://2020.igem.org/Team%3AShanghai_United/Team)
  :   [Collaborations](https://2020.igem.org/Team%3AShanghai_United/Collaborations)
  :   [Attributions](https://2020.igem.org/Team%3AShanghai_United/Attributions)

Notebook

Day1.

* Learning the project and related knowledge;
* Ice breaker games;
* Design team name and logo

Day2.

* Laboratory safety training;
* Prepare LB culture media, sterilized;
* Cultured the strains containing pET28a-vector plasmid and p15a-vector plasmid respectively;

Day3.

* Obtained PPO and P14-

  tyrR-
  Ptyr genes through PCR amplification, agarose gel electrophoresis, and gel extraction;
* Extracted the plasmid pET28a-vector and p15a-vector respectively;

Day4.

* Enzyme digested the plasmid to obtain the linearized vector;
* Connected the DNA fragment and plasmid-vector and transformed into strain;
* Prepared kanamycin and chloramphenicol solution;

Day5.

* Pick bacteria, clone PCR identification;
* Sequence assay;
* Culture the positive clone;

Day6.

* Extracted the constructed plasmid;
* Transformed the plasmid into strain;

Day7.

* IPTG induced the protein expressing;
* Tyr induced the GFP protein expressing;

Day8.

* Purified PPO protein;
* SDS-PAGE Gel Electrophoresis;
* Measured the fluorescence intensity of GFP;

Day9.

* Polyphenol oxidase (PPO) quantification;
* Tyr induced the PPO protein expressing;

Day10.

* Quantified the degradation of p-cresol by PPO；
* Collected the data.

![](https://static.igem.org/mediawiki/2020/6/6f/T--Shanghai_United--notebook01.jpg)

Contact Email: kevinsun763@gmail.com

Wechat: Search "IGEM2020 The Filters" in WeChat

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:Shanghai United/Engineering

非模块化方式使用layui

Entprenuership

![](https://static.igem.org/mediawiki/2020/b/b4/T--Shanghai_United--logo.jpg)

**The Filters**

---

* [Home](https://2020.igem.org/Team%3AShanghai_United)
* Project

  :   [Description](https://2020.igem.org/Team%3AShanghai_United/Description)
  :   [Design](https://2020.igem.org/Team%3AShanghai_United/Design)
  :   [Experiments](https://2020.igem.org/Team%3AShanghai_United/Experiment)
  :   [Notebook](https://2020.igem.org/Team%3AShanghai_United/Notebook)
  :   [Result](https://2020.igem.org/Team%3AShanghai_United/Result)
  :   [Safety](https://2020.igem.org/Team%3AShanghai_United/Safety)
* [Contribution](https://2020.igem.org/Team%3AShanghai_United/Contribution)
* [Engineering](https://2020.igem.org/Team%3AShanghai_United/Engineering)
* [Implementation](https://2020.igem.org/Team%3AShanghai_United/Implementation)
* Parts

  :   [Basic Parts](https://2020.igem.org/Team%3AShanghai_United/Basic%20Parts)
  :   [Composite Parts](https://2020.igem.org/Team%3AShanghai_United/Composite%20Parts)
  :   [Parts Collection](https://2020.igem.org/Team%3AShanghai_United/Parts%20Collection)
* [Model](https://2020.igem.org/Team%3AShanghai_United/Model)
* Human Practice

  :   [Integrated Human Practice](https://2020.igem.org/Team%3AShanghai_United/Human_Practices)
  :   [Fundraising](https://2020.igem.org/Team%3AShanghai_United/Fundraising)
  :   [Entrepreneurship](https://2020.igem.org/Team%3AShanghai_United/Entrepreneurship)
* Team

  :   [Team Member](https://2020.igem.org/Team%3AShanghai_United/Team)
  :   [Collaborations](https://2020.igem.org/Team%3AShanghai_United/Collaborations)
  :   [Attributions](https://2020.igem.org/Team%3AShanghai_United/Attributions)

![](https://static.igem.org/mediawiki/2020/2/2f/T--Shanghai_United--engineering01.jpg)
![](https://static.igem.org/mediawiki/2020/d/df/T--Shanghai_United--engineering02.jpg)
![](https://static.igem.org/mediawiki/2020/c/c1/T--Shanghai_United--engineering03.jpg)
![](https://static.igem.org/mediawiki/2020/6/6c/T--Shanghai_United--engineering04.jpg)
![](https://static.igem.org/mediawiki/2020/1/12/T--Shanghai_United--engineering05.jpg)

Contact Email: kevinsun763@gmail.com

Wechat: Search "IGEM2020 The Filters" in WeChat

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:Shanghai United/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2020/8/8b/HQ_page_logo.jpg)](https://2020.igem.org)

# Team:Shanghai United/Results

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)