iGEM 2016 — Team Ionis_Paris

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# Team:Ionis Paris/Notebook

[![theme logo](https://static.igem.org/mediawiki/2016/7/71/Paris_igem_logo.svg)](https://2016.igem.org/Team%3AIonis_Paris)

* Project
  + [Project description](https://2016.igem.org/Team%3AIonis_Paris/Description)
  + [Biology](https://2016.igem.org/Team%3AIonis_Paris/Biology)
  + [Engineering](https://2016.igem.org/Team%3AIonis_Paris/Hardware)
  + [Applied Design](https://2016.igem.org/Team%3AIonis_Paris/Design)
  + [Entrepreneurship](https://2016.igem.org/Team%3AIonis_Paris/Entrepreneurship)
* In the lab
  + [Working at La Paillasse](https://2016.igem.org/Team%3AIonis_Paris/Working_at_La_Paillasse)
  + [Biobrick design](https://2016.igem.org/Team%3AIonis_Paris/Biobrick_Design)
  + [Cloning Strategy](https://2016.igem.org/Team%3AIonis_Paris/Cloning_Strategy)
  + [Protocols](https://2016.igem.org/Team%3AIonis_Paris/Protocols)
  + [Notebook](https://2016.igem.org/Team%3AIonis_Paris/Notebook)
* Side-projects
  + [CelloCad](https://2016.igem.org/Team%3AIonis_Paris/Measurement)
  + [Collaborations](https://2016.igem.org/Team%3AIonis_Paris/Collaborations)
  + [Mobile application](https://2016.igem.org/Team%3AIonis_Paris/Mobile_application)
  + [Education](https://2016.igem.org/Team%3AIonis_Paris/Engagement)
* Results
  + [Parts and Characterization](https://2016.igem.org/Team%3AIonis_Paris/Parts)
  + [Proof of concept](https://2016.igem.org/Team%3AIonis_Paris/Proof)
  + [Demonstration](https://2016.igem.org/Team%3AIonis_Paris/Demonstrate)
* Human Practices
  + [Overview](https://2016.igem.org/Team%3AIonis_Paris/HPintro)
  + [Project Genesis](https://2016.igem.org/Team%3AIonis_Paris/Quantifly)
  + [Air Pollution](https://2016.igem.org/Team%3AIonis_Paris/Air_pollution)
  + [Opinion Survey](https://2016.igem.org/Team%3AIonis_Paris/Survey)
  + [Ethics and Biosafety](https://2016.igem.org/Team%3AIonis_Paris/Ethics)
  + [Our Actions](https://2016.igem.org/Team%3AIonis_Paris/IntegratedHP)
* Safety
  + [Lab safety](https://2016.igem.org/Team%3AIonis_Paris/Labsafety)
  + [Project safety](https://2016.igem.org/Team%3AIonis_Paris/Projectsafety)
* Team
  + [The team](https://2016.igem.org/Team%3AIonis_Paris/Team)
  + [Events](https://2016.igem.org/Team%3AIonis_Paris/Events)
  + [Attributions](https://2016.igem.org/Team%3AIonis_Paris/Attributions)
  + [Acknowledgement (Sponsors and advisors)](https://2016.igem.org/Team%3AIonis_Paris/Acknowledgement)
  + [Prizes](https://2016.igem.org/Team%3AIonis_Paris/Prices)

* [Project](#body)
  + [Home Page 1](index.html)
  + [Home Page 2](index-2.html)
  + [Home Page 3](index-3.html)
  + [Home Page 4](index-4.html)
  + [Home Page 5](index-5.html)
  + [Home Page 6](index-6.html)
  + [Home Page 7](index-7.html)
  + [Home Page 8](index-8.html)
  + [Home Page 9](index-9.html)
* [Services](#services)
* [About](#about)
* [Portfolio](#Portfolio)
  + [Protfolio 1](protfolio-1.html)
  + [Protfolio 2](protfolio-2.html)
  + [Protfolio 3](protfolio-3.html)
  + [Case Study](case_study.html)
* [Blog](#blog)
  + [Blog Grid](blog_grid.html)
  + [Blog Left Sidebar](blog-left_sidebar.html)
  + [Blog Right Sidebar](blog-right_sidebar.html)
  + [Single Blog Full](single_blog-full.html)
  + [Single Blog Left Sidebar](single_blog-left_sidebar.html)
  + [Single Blog Right Sidebar](single_blog-right_sidebar.html)
* [Contact US](#contact)

# Notebook

#### Competent cells and DNA transformation efficiency tests

* [05/06/2016: Competent cells: E.Coli DH5⍺](https://2016.igem.org/Team%3AIonis_Paris/05_06_16)
* [06/06/2016: Transformation efficiency test : Competent DH5⍺ cells with pSB1C3-RFP plasmid](https://2016.igem.org/Team%3AIonis_Paris/06_06_16)
* [09/06/2016: Competent cells: E.coli BL21](https://2016.igem.org/Team%3AIonis_Paris/09_06_16)
* [10/06/2016: Transformation efficiency test : Competent BL21 cells with pSB1C3-RFP plasmid](https://2016.igem.org/Team%3AIonis_Paris/10_06_16)
* [23/07/2016 : Competent cells DH5⍺](https://2016.igem.org/Team%3AIonis_Paris/23_07_16)
* [25/07/2016 : BB1/BB2: PCRcol \_ Transfo. efficiency test on DH5⍺](https://2016.igem.org/Team%3AIonis_Paris/25_07_16)

#### Amplification of geneBlocks DNA fragments

* [16/06/2016: PCR on P1](https://2016.igem.org/Team%3AIonis_Paris/16_06_16)
* [28/06/2016 : PCR on P2](https://2016.igem.org/Team%3AIonis_Paris/28_06_16)
* [05/07/2016: PCR on P3](https://2016.igem.org/Team%3AIonis_Paris/05_07_16)

#### Biosensor construction

* [15/07/2016 : pSB1C3-RFP: Transfo.](https://2016.igem.org/Team%3AIonis_Paris/15_07_16)
* [17/07/2016 : pSB1C3-RFP Mini-preparation](https://2016.igem.org/Team%3AIonis_Paris/17_07_16)
* [20/07/2016 : BB1/BB2/BB3 Digestion & Ligation](https://2016.igem.org/Team%3AIonis_Paris/20_07_16)
* [21/07/2016 : BB1/BB2/BB3 Transformation](https://2016.igem.org/Team%3AIonis_Paris/21_07_16)
* [25/07/2016 : BB1/BB2: PCRcol \_ Transfo. efficiency test on DH5⍺](https://2016.igem.org/Team%3AIonis_Paris/25_07_16)
* [26/07/2016 : BB2/BB3: Di/Li \_ BB1: Miniprep](https://2016.igem.org/Team%3AIonis_Paris/26_07_16)
* [27/07/2016 : BB12: Di/Li/Tr \_ BB2/BB3: Tr](https://2016.igem.org/Team%3AIonis_Paris/27_07_16)
* [28/07/2016 : BB12/BB2/BB3: PCRcol](https://2016.igem.org/Team%3AIonis_Paris/28_07_16)
* [29/07/2016 : BB12/BB2/BB3: Miniprep](https://2016.igem.org/Team%3AIonis_Paris/29_07_16)
* [02/08/2016 : BB123: Di-Li-Tr](https://2016.igem.org/Team%3AIonis_Paris/02_08_16)
* [03/08/2016 : BB123: PCRcol](https://2016.igem.org/Team%3AIonis_Paris/03_08_16)
* [04/08/2016 : BB123: Miniprep](https://2016.igem.org/Team%3AIonis_Paris/04_08_16)
* [05/09/2016 : BB12mut/BB123mut: SDM-Tr](https://2016.igem.org/Team%3AIonis_Paris/05_09_16)
* [06/09/2016 : BB12mut/BB123mut: PCRcol](https://2016.igem.org/Team%3AIonis_Paris/06_09_16)
* [07/09/2016 :Mini prep: on DH5⍺ transformed with BB12mut and BB123mut](https://2016.igem.org/Team%3AIonis_Paris/07_09_16)
* [17/09/2016 :PCR : on PA and PB](https://2016.igem.org/Team%3AIonis_Paris/17_09_16)
* [18/09/2016 : Digestion: PA and PB](https://2016.igem.org/Team%3AIonis_Paris/18_09_16)
* [19/09/2016 : BBA/BBB: Digestion/Ligation](https://2016.igem.org/Team%3AIonis_Paris/19_09_16)
* [20/09/2016 : BBA/BBB: Transformation](https://2016.igem.org/Team%3AIonis_Paris/20_09_16)
* [21/09/2016 : BBA/BBB: PCR Colony](https://2016.igem.org/Team%3AIonis_Paris/21_09_16)
* [22/09/2016 : BBA/BBB: Miniprep](https://2016.igem.org/Team%3AIonis_Paris/22_09_16)
* [17/10/2016 : BB2mut/BBX2: PCR ▪︎ BB2mut/BBX2/BBC4/BBG2: Di-Li-Tr](https://2016.igem.org/Team%3AIonis_Paris/17_10_2016)
* [18/10/2016 : BB2mut/BBX2/BBC4/BBG2](https://2016.igem.org/Team%3AIonis_Paris/18_10_16)
* [19/10/2016 : BB2mut/BBC4/BBG2: Miniprep ▪︎ BBX2: Di-Li-Tr](https://2016.igem.org/Team%3AIonis_Paris/19_10_16)

#### Toluene/Bioluminescence assays

* [29/09/2016: GLuc - Toluene bioluminescence assay](https://2016.igem.org/Team%3AIonis_Paris/Notebook/29_09_16)
* [18/10/2016: XylR Synthesis Test](https://2016.igem.org/Team%3AIonis_Paris/Notebook/18_10_16bis)

#### CelloCad biobricks construction

* [08/09/2016: C1 and C2 PCR](https://2016.igem.org/Team%3AIonis_Paris/08_09_16)
* [11/09/2016: C1 and C2 PCR](https://2016.igem.org/Team%3AIonis_Paris/Notebook/11_09_16)
* [12/09/2016: C1 and C2 PCR](https://2016.igem.org/Team%3AIonis_Paris/Notebook/12_09_16)
* [01/10/2016: Transformation: competent DH5⍺ cells with BBX4, BBC5 and BBC1](https://2016.igem.org/Team%3AIonis_Paris/Notebook/01_10_2016)
* [03/10/2016: BBX4, BBC5 & BBC1 Miniprep](https://2016.igem.org/Team%3AIonis_Paris/Notebook/03_10_2016)
* [04/10/2016: BBC2 digestion, ligation and transformation](https://2016.igem.org/Team%3AIonis_Paris/Notebook/04_10_2016)
* [05/10/2016: BBC2 PCR colony](https://2016.igem.org/Team%3AIonis_Paris/Notebook/05_10_2016)
* [06/10/2016: BBC2 miniprep, BBX5 digestion and BB-B0015 transformation](https://2016.igem.org/Team%3AIonis_Paris/Notebook/06_10_2016)
* [08/10/2016: Experiment title](https://2016.igem.org/Team%3AIonis_Paris/Notebook/08_10_2016)
* [10/10/2016: BBG3: PCR ▪︎ G1: Miniprep ▪︎ BBG3/BBX3/BBX5/pSB1A2-C45: Di-Li-Tr](https://2016.igem.org/Team%3AIonis_Paris/Notebook/10_10_2016)
* [11/10/2016: BBX3, BBX5, pSB1A2-C45 and BBG3 colony PCR](https://2016.igem.org/Team%3AIonis_Paris/Notebook/11_10_2016)
* [12/10/2016: BBX3, BBX5, pSB1A2-C45 and BBG3 miniprep; BBX5, BB-B0015, pSB1A2-C45, pSB1C3, BBA digestions; C45 into pSB1C3, C45 into BBA, B0015 into BBX5 ligations; BBC45, BBC3, BBX1 transformations](https://2016.igem.org/Team%3AIonis_Paris/Notebook/12_10_2016)
* [13/10/2016: BBC45, BBC3 and BBX1 colony PCR](https://2016.igem.org/Team%3AIonis_Paris/Notebook/13_10_2016)
* [14/10/2016: BBC45, BBC3, BBX1 miniprep](https://2016.igem.org/Team%3AIonis_Paris/Notebook/14_10_2016)
* [17/10/2016: BBP2mut/BBX2: PCR ▪︎ BBP2mut/BBX2/BBC4/BBG2: Di-Li-Tr](https://2016.igem.org/Team%3AIonis_Paris/Notebook/17_10_2016)
* [18/10/2016: BB2mut/BBX2/BBC4/BBG2](https://2016.igem.org/Team%3AIonis_Paris/Notebook/18_10_2016)
* [19/10/2016 : BB2mut/BBC4/BBG2: Miniprep ▪︎ BBX2: Di-Li-Tr](https://2016.igem.org/Team%3AIonis_Paris/19_10_16)

#### Supplementary biobrick construction

* [01/10/2016: Transformation: competent DH5⍺ cells with BBX4, BBC5 and BBC1](https://2016.igem.org/Team%3AIonis_Paris/Notebook/01_10_2016)
* [03/10/2016: BBX4, BBC5 & BBC1 Miniprep](https://2016.igem.org/Team%3AIonis_Paris/Notebook/03_10_2016)
* [06/10/2016: BBC2 miniprep, BBX5 digestion and BB-B0015 transformation](https://2016.igem.org/Team%3AIonis_Paris/Notebook/06_10_2016)
* [07/10/2016: BBX4 miniprep](https://2016.igem.org/Team%3AIonis_Paris/Notebook/07_10_2016)
* [08/10/2016: Experiment title](https://2016.igem.org/Team%3AIonis_Paris/Notebook/08_10_2016)
* [09/10/2016: BBG1 PCR colony](https://2016.igem.org/Team%3AIonis_Paris/Notebook/09_10_2016)
* [10/10/2016: BBG3: PCR ▪︎ G1: Miniprep ▪︎ BBG3/BBX3/BBX5/pSB1A2-C45: Di-Li-Tr](https://2016.igem.org/Team%3AIonis_Paris/Notebook/10_10_2016)
* [11/10/2016: BBX3, BBX5, pSB1A2-C45 and BBG3 colony PCR](https://2016.igem.org/Team%3AIonis_Paris/Notebook/11_10_2016)
* [12/10/2016: BBX3, BBX5, pSB1A2-C45 and BBG3 miniprep; BBX5, BB-B0015, pSB1A2-C45, pSB1C3, BBA digestions; C45 into pSB1C3, C45 into BBA, B0015 into BBX5 ligations; BBC45, BBC3, BBX1 transformations](https://2016.igem.org/Team%3AIonis_Paris/Notebook/12_10_2016)
* [13/10/2016: BBC45, BBC3 and BBX1 colony PCR](https://2016.igem.org/Team%3AIonis_Paris/Notebook/13_10_2016)
* [14/10/2016: BBC45, BBC3, BBX1 miniprep](https://2016.igem.org/Team%3AIonis_Paris/Notebook/14_10_2016)
* [17/10/2016: BBP2mut/BBX2: PCR ▪︎ BBP2mut/BBX2/BBC4/BBG2: Di-Li-Tr](https://2016.igem.org/Team%3AIonis_Paris/Notebook/17_10_2016)
* [18/10/2016: BB2mut/BBX2/BBC4/BBG2](https://2016.igem.org/Team%3AIonis_Paris/Notebook/18_10_2016)
* [19/10/2016 : BB2mut/BBC4/BBG2: Miniprep ▪︎ BBX2: Di-Li-Tr](https://2016.igem.org/Team%3AIonis_Paris/19_10_16)

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#### iGEM IONIS

We're a group of six different schools from the IONIS Education Group. For this
competition we wanted to take advantages of the multiple schools and activity field
given by the IONIS education group to create a solid project.

[Read More](https://2016.igem.org/Team%3AIonis_Paris/Team)

#### Stay in touch

* Location: 66 Rue Guy Môquet, 94800 Villejuif, France
* email: igem.ionis@gmail.com

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* [Home](https://2016.igem.org/Team%3AIonis_Paris)
* [in the Lab](https://2016.igem.org/Team%3AIonis_Paris/Working_at_La_Paillasse)
* [Side Projects](https://2016.igem.org/Team%3AIonis_Paris/Measurement)
* [Results](https://2016.igem.org/Team%3AIonis_Paris/Parts)
* [Human
  Practice](https://2016.igem.org/Team%3AIonis_Paris/HPintro)
* [Team](https://2016.igem.org/Team%3AIonis_Paris/Team)

©IONIS\_IGEM\_2016.

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