iGEM 2018 — Team Saint_Joseph

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# Team:Saint Joseph/Notebook

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[HOME](https://2018.igem.org/Team%3ASaint_Joseph)

TEAM

[Team Members](https://2018.igem.org/Team%3ASaint_Joseph/Team)
[Collaborations](https://2018.igem.org/Team%3ASaint_Joseph/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ASaint_Joseph/Description)
[Design](https://2018.igem.org/Team%3ASaint_Joseph/Design)
[Experiments](https://2018.igem.org/Team%3ASaint_Joseph/Experiments)
[Notebook](https://2018.igem.org/Team%3ASaint_Joseph/Notebook)
[InterLab](https://2018.igem.org/Team%3ASaint_Joseph/InterLab)
[Model](https://2018.igem.org/Team%3ASaint_Joseph/Model)
[Results](https://2018.igem.org/Team%3ASaint_Joseph/Results)
[Demonstrate](https://2018.igem.org/Team%3ASaint_Joseph/Demonstrate)
[Improve](https://2018.igem.org/Team%3ASaint_Joseph/Improve)
[Attributions](https://2018.igem.org/Team%3ASaint_Joseph/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ASaint_Joseph/Parts)
[Basic Parts](https://2018.igem.org/Team%3ASaint_Joseph/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ASaint_Joseph/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ASaint_Joseph/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ASaint_Joseph/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ASaint_Joseph/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ASaint_Joseph/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ASaint_Joseph/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ASaint_Joseph/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ASaint_Joseph/Hardware)
[Measurement](https://2018.igem.org/Team%3ASaint_Joseph/Measurement)
[Model](https://2018.igem.org/Team%3ASaint_Joseph/Model)
[Plant](https://2018.igem.org/Team%3ASaint_Joseph/Plant)
[Software](https://2018.igem.org/Team%3ASaint_Joseph/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=Saint_Joseph)

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:Saint Joseph/Experiments

# Experiments

Unfortunately, we couldn’t conduct our experiments due to various reasons. However, we developed the concept of the experiments we wanted to conduct, so we will explain the theoretic basis of our project.

---

We planned to test various interactions between phages, the bacteria Vibrio anguillarum, antibiotics and AMP Pleurocidin. By using the in-vitro environments below:

![](https://static.igem.org/mediawiki/2018/3/36/T--Saint_Joseph--experiments.png)

#### Saint Joseph RAFI

![](https://static.igem.org/mediawiki/2018/a/ad/T--Saint_Joseph--logo-sj-igem.jpeg)

##### Caferağa mah, Dr. Esat Işık cd, 66/11

###### Kadıköy, İstanbul

[![](https://image.flaticon.com/icons/svg/174/174855.svg)](https://www.instagram.com/igem_saintjoseph/)
![](https://image.flaticon.com/icons/svg/281/281769.svg)

#### Quick Links

##### Project

[###### Background](https://2018.igem.org/Team%3ASaint_Joseph/Background)
[###### Description](https://2018.igem.org/Team%3ASaint_Joseph/Description)

##### Human Practices

[###### Human Practices](https://2018.igem.org/Team%3ASaint_Joseph/Human_Practices)

##### Team

[###### Team](https://2018.igem.org/Team%3ASaint_Joseph/Team)
[###### Collaborations](https://2018.igem.org/Team%3ASaint_Joseph/Collaborations)
[###### Sponsors](https://2018.igem.org/Team%3ASaint_Joseph/sponsors)
[###### Attributions](https://2018.igem.org/Team%3ASaint_Joseph/Attributions)

Saint Joseph IGEM 2018 ©

 [![](https://static.igem.org/mediawiki/2018/f/fd/T--Saint_Joseph--logo-rafi-igem.png)](/Team%3ASaint_Joseph)

* [Project](http://example.com)

  [Background](https://2018.igem.org/Team%3ASaint_Joseph/Background)
  [Description](https://2018.igem.org/Team%3ASaint_Joseph/Description)
* [Lab](http://example.com)

  [Protocols](https://2018.igem.org/Team%3ASaint_Joseph/Protocol)
  [Experiments](https://2018.igem.org/Team%3ASaint_Joseph/Experiments)
  [Safety](https://2018.igem.org/Team%3ASaint_Joseph/Safety)
  [Interlab](https://2018.igem.org/Team%3ASaint_Joseph/InterLab)
* [Human Practices](http://example.com)

  [Human Practices](https://2018.igem.org/Team%3ASaint_Joseph/Human_Practices)
* [Team](http://example.com)

  [Our Team](https://2018.igem.org/Team%3ASaint_Joseph/Team)
  [Collaborations](https://2018.igem.org/Team%3ASaint_Joseph/Collaborations)
  [Sponsors](https://2018.igem.org/Team%3ASaint_Joseph/Sponsors)
  [Attributions](https://2018.igem.org/Team%3ASaint_Joseph/Attributions)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:Saint Joseph/Results

# Results

Substance < style... oh wait

Here we show our PCR results, and the conditions used, for the fragments which we subsequently used in Gibson assembly, as well as the sequencing results for our part, NrfB.

---

## PCR results

The following table shows the final conditions used for successful PCR:

![](https://static.igem.org/mediawiki/2017/6/69/T--Bristol--FinalPCRConditions.png)

An agarose gel of final fragment PCR products is shown below:

![](https://static.igem.org/mediawiki/2017/6/6c/T--Bristol--PCRAllFragments.png)

---

## Sequencing results

We successfully performed PCR on our fragments with our editing primers, to isolate the whole coding regions. Sequencing of our constructs showed that most had significant deletions and insertions of unknown DNA, however one construct, NrfB pSB1C3, had
only one point mutation (shown below). We submitted this part (see our [Parts](https://2017.igem.org/Team%3ABristol/Parts#part) page), annotating the mutation so future teams can take it into consideration when
ordering the part. The part can still be used accurately if this mutation is edited, either molecularly, or through DNA synthesis.

![](https://static.igem.org/mediawiki/2017/5/5d/T--Bristol--SequencingResults.png)

#### Saint Joseph RAFI

![](https://static.igem.org/mediawiki/2017/5/5d/T--Bristol--TeamLogo.svg)

##### Caferağa mah, Dr. Esat Işık cd, 66/11

###### Kadıköy, İstanbul

[![](https://image.flaticon.com/icons/svg/174/174855.svg)](https://www.instagram.com/igem_saintjoseph/)
![](https://image.flaticon.com/icons/svg/281/281769.svg)

#### Quick Links

##### Project

[###### Background](https://2018.igem.org/Team%3ASaint_Joseph/Background)
[###### Description](https://2018.igem.org/Team%3ASaint_Joseph/Description)

##### Human Practices

[###### Human Practices](https://2018.igem.org/Team%3ASaint_Joseph/Human_Practices)
[###### Public Engagement](https://2018.igem.org/Team%3ASaint_Joseph/Public_Engagement)

##### Team

[###### Team](https://2018.igem.org/Team%3ASaint_Joseph/Team)
[###### Collaborations](/https%3A//2018.igem.org/Team%3ASaint_Joseph/Collaborations)
[###### Sponsors](https://2018.igem.org/Team%3ASaint_Joseph/Sponsors)
[###### Attributions](https://2018.igem.org/Team%3ASaint_Joseph/Attributions)

#### Sponsors

Saint Joseph IGEM 2018©

 [![](https://static.igem.org/mediawiki/2018/f/fd/T--Saint_Joseph--logo-rafi-igem.png)](/Team%3ASaint_Joseph)

* [Project](http://example.com)

  [Background](https://2018.igem.org/Team%3ASaint_Joseph/Background)
  [Description](https://2018.igem.org/Team%3ASaint_Joseph/Description)
* [Lab](http://example.com)

  [Protocols](https://2018.igem.org/Team%3ASaint_Joseph/Protocol)
  [Experiments](https://2018.igem.org/Team%3ASaint_Joseph/Experiments)
  [Results](https://2018.igem.org/Team%3ASaint_Joseph/Results)
  [Safety](https://2018.igem.org/Team%3ASaint_Joseph/Safety)
  [Interlab](https://2018.igem.org/Team%3ASaint_Joseph/InterLab)
* [Human Practices](http://example.com)

  [Human Practices](https://2018.igem.org/Team%3ASaint_Joseph/Human_Practices)
  [Public Engagement](/Team%3ABristol/Engagement)
* [Team](http://example.com)

  [Our Team](https://2018.igem.org/Team%3ASaint_Joseph/Team)
  [Collaborations](https://2018.igem.org/Team%3ASaint_Joseph/Collaborations)
  [Sponsors](https://2018.igem.org/Team%3ASaint_Joseph/Sponsors)
  [Attributions](https://2018.igem.org/Team%3ASaint_Joseph/Attributions)