iGEM 2017 — Team CIEI-BJ

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# Team:CIEI-BJ/Notebook

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[Home](https://2017.igem.org/Team%3ACIEI-BJ)

Project

[Description](https://2017.igem.org/Team%3ACIEI-BJ/Description)

[Background](https://2017.igem.org/Team%3ACIEI-BJ/Background)

[Our Design](https://2017.igem.org/Team%3ACIEI-BJ/Design)

[Results](https://2017.igem.org/Team%3ACIEI-BJ/Results)

[Parts](https://2017.igem.org/Team%3ACIEI-BJ/Parts)

Human Practice

[Overview](https://2017.igem.org/Team%3ACIEI-BJ/HP/Overview)

[Silver](https://2017.igem.org/Team%3ACIEI-BJ/HP/Silver)

[Gold](https://2017.igem.org/Team%3ACIEI-BJ/HP/Gold_Integrated)

[Collaboration](https://2017.igem.org/Team%3ACIEI-BJ/Collaborations)

[Attribution](https://2017.igem.org/Team%3ACIEI-BJ/Attributions)

[Achievement](https://2017.igem.org/Team%3ACIEI-BJ/Achievement)

Notebook

[Protocol](https://2017.igem.org/Team%3ACIEI-BJ/Protocol)

[Notebook](https://2017.igem.org/Team%3ACIEI-BJ/Notebook)

[Safety](https://2017.igem.org/Team%3ACIEI-BJ/Safety)

[Team](https://2017.igem.org/Team%3ACIEI-BJ/Team)

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* [Feb. 2017](#a1)* [Mar. 2017](#a2)* [Apr. 2017](#a3)* [May. 2017](#a4)* [Jun. 2017](#a5)* [Jul.2017](#a6)
            + [Week 1-2](#a7)
            + [Week 3-4](#a8)
          * [Aug.2017](#a9)
            + [Week 1-2](#a10)
            + [Week 3-4](#a11)
          * [Sept. 2017](#a12)* [Oct. 2017](#a13)* [Nov. 2017](#a14)

Feb. 2017

-We learnt some basic concepts of synthetic biology, and the safety rule of laboratory.

-We practiced some basic experimental operation under guidance of Dr. Wang from China Agriculture University.

-We read a lot of literatures, brainstormed and came up with the topic of our project.

Mar. 2017

-After massive literature study and discussion, our group members agreed to design a toxic-free product which could repel mosquitos effectively.

-We visited Institute of Zoology, Chinese Academy of Sciences and consulted with the professors about our experimental plans.

-We decided the research plan which is most possible to lead us a successful accomplishment of our research objective.

-We presented our ideas to some teachers from Institute of Agriculture, and gained several useful advice.

Apr. 2017

-Based on group communication, our project aimed on citronellol, because the literature study gave us a clear clue about the incomparable advantages of citronellol in the perspective of repelling mosquitoes.

-We searched more documents about citronello and its effects on mosquito repelling. Finally we found out that OYE and GES are the two key enzymes in citronellol production pathway.

-We designed our unique genetic circuits and selected the appropriate vector which can result in a successful expression of our target proteins.

-We cloned both gene OYE and GES after the primers was designed.

May. 2017

-We visited the College of Life Science in Peking University and asked Prof. Liu for some advice on our plan.

-We Found and chose the appropriate vector for our project which can express our target protein in yeast.

-We Found and chose the appropriate vector for our project which can express our target protein in E.coli.

Jun. 2017

-We kept searching for literature and improved our research plan based on Prof. Liu’s suggestions.

-We combined GES and OYE with 2A through PCR successfully.

Jul.2017

Week 1-2

-We visited the Chinese Center for Disease Control and Prevention and discussed our project with the experts there.

-We learnt that diseases caused by mosquitos are prevailing all over the world and there is an urgent need for a more effective repellent product.

Week 3-4

-We started to write our background, experiment designs, and experiment protocols.

-We ligated GES and OYE on the yeast expression vector pPIC9K separately, and on E. Coli expression vector pPET32a separately as well.

Aug.2017

Week 1-2

-We designed the questionnaires and set up a public account on WeChat to disseminate our projects.

-We combined the two yeast expression vectors (pPIC9K) together to form the combination of GES+2A+OYE

Week 3-4

-We furthered our experiments under guidance of Dr. Song to build up other vectors.

-We started our Wiki designing.

Sept. 2017

-We transformed the different vectors into yeast and E. Coli separately.

-We developed yeast and E. Coli under different conditions to maximize the efficiencies.

Oct. 2017

-We purified our final product citronello through bio-chemical methods for the further experiments.

-We designed and conducted human body test for the effectiveness of citronello, to prove the usefulness of our product.

-We accomplished the majority of the experiments in laboratory and began to analyze our results.

-To search for concrete suggestions, we also held a communicating symposium, inviting teachers in the genetic engineering field to provide some suggestions for improvement of the our experiments and inspire us with some creative ideas.

Nov. 2017

-We finalized everything related to the project and prepared for the Giant Jamboree in Boston.

Sponsors

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