iGEM 2015 — Team Colegio_EmelinaU

# Team:Colegio EmelinaU/Notebook

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# Journal of Laboratory

## May 30th, 2015

* Genetic transformation of red coloration [B0010]
* Plasmid removing: [K909008]= tetR-DBD-UVR8
[B0010]= Terminator
[R0040]= Promoter

## June 13th, 2015

* Genetic transformation of [ B0010 ] \* failed . We make a new attempt
* Electrophoresis : [ K909008 ]
[ B0010 ]
[ R0040 ]

## June 20th, 2015

* Expanded coloring [ B0010 ]
* Make plasmid removing [ B0010 ]
* Perform digests [ B0010 ] = ( Terminator ) Digestion
[ K909008 ] = ( tetR - DBD - UVR8 ) Double digestion* electrophoresis : [ B0010 ] 16746p
[ K909008 ] 21506p

## June 27th, 2015

* Purification : [ B0010 ]
[ K909008 ]* Plasmid removing : [ E1010 ]
* Electrophoresis : [ B0010 ]
[ K909008 ]<
[ E1010 ]

## July 04th, 2015

* Plasmid removing: [J23119]
[B0010]
[K909008]
[R0040]
[E1010]* Electrophoresis: [B0010]
[R0040]
[K909008]
[J23119]* Digestion: [B0010]
[K909008]

## July 13th, 2015

* Electrophoresis
* Plasmid Removing
* Terminator digestion and teR-DBD-UVR8

## July 14th, 2015

* Bands Purification: [K909008]
[B0010]* Join [K909008]
[B0010]* Electroophoresis:
July04th= [K909008];[J23119];[B0010];[R0040]<
[K909008]; [R0040]; [B0010]; [R0040]* Digestion

## July 24th, 2015

 Digestion= [K909008]= Xbal-PSTI
[J23119]= SPEI-PSTI
[B0010]= EcoRI-Xbal
[R0040]= SPEI-PSTI
[E1010]= Xbal-PSTI

## August 1st, 2015

* Digestion: [B0010]= E X I
[R0040]= S P
[K909008]= X P II
[J23119]= SPEI PSTI
[R0040]= S P I
[R0040]= S P I
[K909008]= X P II N
[B0010]= E X I* Genetic Transformation: [J23119]= constitutive promoter
[K1084103]= RBS
[K909008]= tetR-DBD-UVR8
[B0010]= Terminator
[R0040]= Promoter
[K592009]= purple color
[K592011]= blue color

## August 22nd, 2015

* Digestion: [K1084103]
* Purification: [K909008]
* Genetic transformation: [K592009]

## August 29th, 2015

* Plasmid removing: [K592009]
* Purification: [K909008] Black
[K909008] blue