iGEM 2025 — Team izju-china


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{% block lead %}Engineering Success - DBTL Cycles for FoCas Development{% endblock %}
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Overview

![DBTL Overview](https://static.igem.wiki/teams/5913/dbtl/dbtl-overview.webp)

# DBTL1: Target Selection

Design (D)
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Build (B)
{% include "pages/\_engineering\_dbtl1\_build.html" ignore missing %}

Test (T)
{% include "pages/\_engineering\_dbtl1\_test.html" ignore missing %}

Learn (L)
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# DBTL2: CRISPR-mecA Cleavage

Design (D)
{% include "pages/\_engineering\_dbtl2\_design.html" ignore missing %}

Build (B)
{% include "pages/\_engineering\_dbtl2\_build.html" ignore missing %}

Test (T)
{% include "pages/\_engineering\_dbtl2\_test.html" ignore missing %}

Learn (L)
{% include "pages/\_engineering\_dbtl2\_learn.html" ignore missing %}

# DBTL3: Origami-CRISPR Delivery

## 3.0 Origami-CRISPR Delivery

Design (D)
{% include "pages/\_engineering\_dbtl3\_design.html" ignore missing %}

Build (B)
{% include "pages/\_engineering\_dbtl3\_build.html" ignore missing %}

Test (T)
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Learn (L)
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## 3.1 Modify sgRNA

Design (D)
{% include "pages/\_engineering\_dbtl3.1\_design.html" ignore missing %}

Build (B)
{% include "pages/\_engineering\_dbtl3.1\_build.html" ignore missing %}

Test (T)
{% include "pages/\_engineering\_dbtl3.1\_test.html" ignore missing %}

Learn (L)
{% include "pages/\_engineering\_dbtl3.1\_learn.html" ignore missing %}

## 3.2 Modify DNA Origami

Design (D)
{% include "pages/\_engineering\_dbtl3.2\_design.html" ignore missing %}

Build (B)
{% include "pages/\_engineering\_dbtl3.2\_build.html" ignore missing %}

Test (T)
{% include "pages/\_engineering\_dbtl3.2\_test.html" ignore missing %}

Learn (L)
{% include "pages/\_engineering\_dbtl3.2\_learn.html" ignore missing %}

## 3.3.1 FITC-Cas9

Design (D)
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Build (B)
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Test (T)
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Learn (L)
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## 3.3.2 FITC-sgRNAL

Design (D)
{% include "pages/\_engineering\_dbtl3.3.2\_design.html" ignore missing %}

Build (B)
{% include "pages/\_engineering\_dbtl3.3.2\_build.html" ignore missing %}

Test (T)
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Learn (L)
{% include "pages/\_engineering\_dbtl3.3.2\_learn.html" ignore missing %}

## 3.4.1 Gel Electrophoresis Verification

Design (D)
{% include "pages/\_engineering\_dbtl3.4.1\_design.html" ignore missing %}

Build (B)
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Test (T)
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Learn (L)
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## 3.4.2 UV-Vis Absorption Verification

Design (D)
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Build (B)
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Test (T)
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Learn (L)
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# DBTL4: Membrane-Disrupting DNA Origami

## 4.0 Membrane-Disrupting DNA Origami

Design (D)
{% include "pages/\_engineering\_dbtl4\_design.html" ignore missing %}

Build (B)
{% include "pages/\_engineering\_dbtl4\_build.html" ignore missing %}

Test (T)
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Learn (L)
{% include "pages/\_engineering\_dbtl4\_learn.html" ignore missing %}

## 4.1.1 Preliminary NPN Uptake Assay

Design (D)
{% include "pages/\_engineering\_dbtl4.1.1\_design.html" ignore missing %}

Build (B)
{% include "pages/\_engineering\_dbtl4.1.1\_build.html" ignore missing %}

Test (T)
{% include "pages/\_engineering\_dbtl4.1.1\_test.html" ignore missing %}

Learn (L)
{% include "pages/\_engineering\_dbtl4.1.1\_learn.html" ignore missing %}

## 4.1.2 Refined NPN Uptake Assay

Design (D)
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Build (B)
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Test (T)
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Learn (L)
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# DBTL5: Aptamer-Targeted DNA Origami

Design (D)
{% include "pages/\_engineering\_dbtl5\_design.html" ignore missing %}

Build (B)
{% include "pages/\_engineering\_dbtl5\_build.html" ignore missing %}

Test (T)
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Learn (L)
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# DBTL6: Lock-Enhanced DNA Origami

Design (D)
{% include "pages/\_engineering\_dbtl6\_design.html" ignore missing %}

Build (B)
{% include "pages/\_engineering\_dbtl6\_build.html" ignore missing %}

Test (T)
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Learn (L)
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