iGEM 2014 — Team IIT_Delhi

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# Team:IIT Delhi/Notebook

### From 2014.igem.org

iGEM IIT Delhi 2014

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* [Home](https://2014.igem.org/Team%3AIIT_Delhi)
* [Project](https://2014.igem.org/Team%3AIIT_Delhi/Project)
* [Modeling](https://2014.igem.org/Team%3AIIT_Delhi/Modeling)
* [Team](https://2014.igem.org/Team%3AIIT_Delhi/Team)
* [Notebook](https://2014.igem.org/Team%3AIIT_Delhi/Notebook)
* [Attributions](https://2014.igem.org/Team%3AIIT_Delhi/Attributions)
* [Safety](https://2014.igem.org/Team%3AIIT_Delhi/Safety)
* [Parts](https://2014.igem.org/Team%3AIIT_Delhi/Parts)
* [Achievements](https://2014.igem.org/Team%3AIIT_Delhi/Achievements)

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![](https://static.igem.org/mediawiki/2014/1/1a/Notebook_iitd.jpg)

Here's our Notebook- a log in which we have written about what we've been doing in the lab (We're worked hard, We promise !)

* ✔ Week 1,2,3,4: Our initial week's work comprised of sessions in which our team had lab safety training and learned how to implement different protocols. Ex: Genomic DNA Extraction, Gel Electrophoresis etc. [Click here to see the notebook of these weeks](https://static.igem.org/mediawiki/2014/b/be/Igem_iitd_LAB_Week_1-2-3-4.pdf)

* ✔ Week 5-6-7-8: These week's work consists of more protocol implementation. Ex: Restriction Digestion, Ligation, 3A(RFC10) Assembly etc. and it also includes brainstorming the problem solving  [Click here to see the notebook of these weeks](https://static.igem.org/mediawiki/2014/9/99/Igem_iitd_LAB_Week_5-6-7-8.pdf)

* ✔ Week 9-10 : Here we started performing actual experiments involved in the project  [Click here to see the notebook of these weeks](https://static.igem.org/mediawiki/2014/d/df/Igem_iitd_LAB_Week_9-10.pdf)

* ✔ Week 11 : Plasmid Preps, Gel Runs , Restriction digestions and Ligations as our 3A assembly failed initially.  [Click here to see the notebook of this week](https://static.igem.org/mediawiki/2014/4/48/Igem_iitd_LAB_Week_11.pdf)

* ✔ Week 12 : More and more Preps, Runs , Digestions and Ligations.  [Click here to see the notebook of this week](https://static.igem.org/mediawiki/2014/2/2e/Igem_iitd_LAB_Week_12.pdf)

* ✔ Week 13 : More and more Preps, Runs , Digestions and Ligations.  [Click here to see the notebook of this week](https://static.igem.org/mediawiki/2014/6/62/Igem_iitd_LAB_Week_13.pdf)

* ✔ Week 14 : And finally we hit on the target, our first clone , nrfA it is  [Click here to see the notebook of this week](https://static.igem.org/mediawiki/2014/e/e2/Igem_iitd_LAB_Week_14.pdf)

* ✔ Week 16-17-18-19 : Now its time for other clones, More and more preps, runs , digestions, ligations and PCRs [Click here to see the notebook of these weeks](https://static.igem.org/mediawiki/2014/5/58/Igem_iitd_LAB_Week_16-17-18-19.pdf)

* ✔ Week 23-24-25 : And finally we got our clones !!!  [Click here to see the notebook of these weeks](https://static.igem.org/mediawiki/2014/3/3a/Igem_iitd_Week_23-24-25.pdf)

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| [HOME](https://2014.igem.org/Team%3AIIT_Delhi) | | | [PROJECT](https://2014.igem.org/Team%3AIIT_Delhi/Project) | | | [MODELING](https://2014.igem.org/Team%3AIIT_Delhi/Modeling) | | | [TEAM](https://2014.igem.org/Team%3AIIT_Delhi/Team) | | | [NOTEBOOK](https://2014.igem.org/Team%3AIIT_Delhi/Notebook) | | | [ATTRIBUTIONS](https://2014.igem.org/Team%3AIIT_Delhi/Attributions) | | | [SAFETY](https://2014.igem.org/Team%3AIIT_Delhi/Safety) | | | [PARTS](https://2014.igem.org/Team%3AIIT_Delhi/Parts) | | | [ACHIEVEMENTS](https://2014.igem.org/Team%3AIIT_Delhi/Achievements) |

Copyright © iGEM-IIT Delhi 2014 | Developer: ABHISHEK BHARTI & SHASHANK YADAV

"We won a Bronze Medal at the iGEM Giant Jamboree 2014"

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