iGEM 2019 — Team ITESO_Guadalajara

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# Team:ITESO Guadalajara/Notebook

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[HOME](https://2019.igem.org/Team%3AITESO_Guadalajara)

TEAM

[Team Members](https://2019.igem.org/Team%3AITESO_Guadalajara/Team)
[Collaborations](https://2019.igem.org/Team%3AITESO_Guadalajara/Collaborations)

PROJECT

[Description](https://2019.igem.org/Team%3AITESO_Guadalajara/Description)
[Design](https://2019.igem.org/Team%3AITESO_Guadalajara/Design)
[Experiments](https://2019.igem.org/Team%3AITESO_Guadalajara/Experiments)
[Notebook](https://2019.igem.org/Team%3AITESO_Guadalajara/Notebook)
[Contribution](https://2019.igem.org/Team%3AITESO_Guadalajara/Contribution)
[Results](https://2019.igem.org/Team%3AITESO_Guadalajara/Results)
[Demonstrate](https://2019.igem.org/Team%3AITESO_Guadalajara/Demonstrate)
[Improve](https://2019.igem.org/Team%3AITESO_Guadalajara/Improve)
[Attributions](https://2019.igem.org/Team%3AITESO_Guadalajara/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3AITESO_Guadalajara/Parts)
[Basic Parts](https://2019.igem.org/Team%3AITESO_Guadalajara/Basic_Part)
[Composite Parts](https://2019.igem.org/Team%3AITESO_Guadalajara/Composite_Part)
[Part Collection](https://2019.igem.org/Team%3AITESO_Guadalajara/Part_Collection)

[SAFETY](https://2019.igem.org/Team%3AITESO_Guadalajara/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3AITESO_Guadalajara/Human_Practices)
[Education & Engagement](https://2019.igem.org/Team%3AITESO_Guadalajara/Public_Engagement)

AWARDS

[Entrepreneurship](https://2019.igem.org/Team%3AITESO_Guadalajara/Entrepreneurship)
[Hardware](https://2019.igem.org/Team%3AITESO_Guadalajara/Hardware)
[Measurement

[Software](https://2019.igem.org/Team%3AITESO_Guadalajara/Software)
[Plant](https://2019.igem.org/Team%3AITESO_Guadalajara/Plant)](https://2019.igem.org/Team%3AITESO_Guadalajara/Measurement)

[JUDGING FORM ⇗](https://igem.org/2019_Judging_Form?team=ITESO_Guadalajara)

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)


[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:ITESO Guadalajara/Experiments

![](https://static.igem.org/mediawiki/2019/7/7b/T--ITESO_Guadalajara--arribota.png)* Team
  + [Team Members](https://2019.igem.org/Team%3AITESO_Guadalajara/Team)
  + [Collaborations](https://2019.igem.org/Team%3AITESO_Guadalajara/Collaborations)
* [Project](https://2019.igem.org/Team%3AITESO_Guadalajara/Design)
  + [Description](https://2019.igem.org/Team%3AITESO_Guadalajara/Description)
  + [Design](https://2019.igem.org/Team%3AITESO_Guadalajara/Design)
  + [Experiments](https://2019.igem.org/Team%3AITESO_Guadalajara/Experiments)
  + [Notebook](https://2019.igem.org/Team%3AITESO_Guadalajara/Notebook)
  + [Results](https://2019.igem.org/Team%3AITESO_Guadalajara/Results)
  + [Attributions](https://2019.igem.org/Team%3AITESO_Guadalajara/Attributions)
* [Parts](https://2019.igem.org/Team%3AITESO_Guadalajara/Parts)
  + [Basic Parts](https://2019.igem.org/Team%3AITESO_Guadalajara/Basic_Part)
  + [Composite Parts](https://2019.igem.org/Team%3AITESO_Guadalajara/Composite_Part)
* [Human Practices](https://2019.igem.org/Team%3AITESO_Guadalajara/Human_Practices)
  + [Integrated Human Practices](https://2019.igem.org/Team%3AITESO_Guadalajara/Human_Practices)
  + [Public Engagement and Education](https://2019.igem.org/Team%3AITESO_Guadalajara/Public_Engagement)* Awards
    + [Entrepreneurship](https://2019.igem.org/Team%3AITESO_Guadalajara/Entrepreneurship)
    + [Hardware](https://2019.igem.org/Team%3AITESO_Guadalajara/Hardware)
    + [Plant](https://2019.igem.org/Team%3AITESO_Guadalajara/Plant)
* [Home](https://2019.igem.org/Team%3AITESO_Guadalajara)

# Protocols

![](https://static.igem.org/mediawiki/2019/0/07/T--ITESO_Guadalajara--Experiments.png)

Here we present the protocols we used to develop the experimental part of our project.
 Feel free to download them.
If you have any doubt or comment about a protocol, you can contact us through our social media, we will be more than glad to help you out.

Preparation of culture media

[RubisCO\_CM.pdf](https://static.igem.org/mediawiki/2019/c/cc/T--ITESO_Guadalajara--protocols1.pdf)

Reactivation and growth of Synechococcus sp.

[RubisCO\_CM.pdf](https://static.igem.org/mediawiki/2019/a/a4/T--ITESO_Guadalajara--protocols2.pdf)

Determination of dissolved CO2 (aqueous and gas)

[RubisCO\_CM.pdf](https://static.igem.org/mediawiki/2019/b/b1/T--ITESO_Guadalajara--Protocols%203.pdf)

Electrophoresis

[RubisCO\_CM.pdf](https://static.igem.org/mediawiki/2019/e/ec/T--ITESO_Guadalajara--protocols4.pdf)

CMP\_E.coli

[RubisCO\_CM.pdf](https://static.igem.org/mediawiki/2019/1/17/T--ITESO_Guadalajara--protocols5.pdf)

Transformation

[RubisCO\_CM.pdf](https://static.igem.org/mediawiki/2019/0/01/T--ITESO_Guadalajara--protocols6.pdf)

DNA Activation

[RubisCO\_CM.pdf](https://static.igem.org/mediawiki/2019/8/83/T--ITESO_Guadalajara--protocols7.pdf)

Protocol colony PCR and Restriction Digest

[RubisCO\_CM.pdf](https://static.igem.org/mediawiki/2019/4/47/T--ITESO_Guadalajara--protocols8.pdf)

Protocol Genomic DNA extraction for Synechococcus sp

<RubisCO_CM.pdf>

Protocol Ligation

[RubisCO\_CM.pdf](https://static.igem.org/mediawiki/2019/b/b7/T--ITESO_Guadalajara--protocols9.pdf)

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:ITESO Guadalajara/Results

![](https://static.igem.org/mediawiki/2019/7/7b/T--ITESO_Guadalajara--arribota.png)* Team
  + [Team Members](https://2019.igem.org/Team%3AITESO_Guadalajara/Team)
  + [Collaborations](https://2019.igem.org/Team%3AITESO_Guadalajara/Collaborations)
* [Project](https://2019.igem.org/Team%3AITESO_Guadalajara/Design)
  + [Description](https://2019.igem.org/Team%3AITESO_Guadalajara/Description)
  + [Design](https://2019.igem.org/Team%3AITESO_Guadalajara/Design)
  + [Experiments](https://2019.igem.org/Team%3AITESO_Guadalajara/Experiments)
  + [Notebook](https://2019.igem.org/Team%3AITESO_Guadalajara/Notebook)
  + [Results](https://2019.igem.org/Team%3AITESO_Guadalajara/Results)
  + [Attributions](https://2019.igem.org/Team%3AITESO_Guadalajara/Attributions)
* [Parts](https://2019.igem.org/Team%3AITESO_Guadalajara/Parts)
  + [Basic Parts](https://2019.igem.org/Team%3AITESO_Guadalajara/Basic_Part)
  + [Composite Parts](https://2019.igem.org/Team%3AITESO_Guadalajara/Composite_Part)
* [Human Practices](https://2019.igem.org/Team%3AITESO_Guadalajara/Human_Practices)
  + [Integrated Human Practices](https://2019.igem.org/Team%3AITESO_Guadalajara/Human_Practices)
  + [Public Engagement and Education](https://2019.igem.org/Team%3AITESO_Guadalajara/Public_Engagement)* Awards
    + [Entrepreneurship](https://2019.igem.org/Team%3AITESO_Guadalajara/Entrepreneurship)
    + [Hardware](https://2019.igem.org/Team%3AITESO_Guadalajara/Hardware)
    + [Plant](https://2019.igem.org/Team%3AITESO_Guadalajara/Plant)
* [Home](https://2019.igem.org/Team%3AITESO_Guadalajara)

# Results

![](https://static.igem.org/mediawiki/2019/c/cf/T--ITESO_Guadalajara--Gel1.jpg)
![](https://static.igem.org/mediawiki/2019/4/4c/T--ITESO_Guadalajara--lane.jpg)
![](https://static.igem.org/mediawiki/2019/1/11/T--ITESO_Guadalajara--gel2.jpg)
![](https://static.igem.org/mediawiki/2019/b/b1/T--ITESO_Guadalajara--lane2.jpg)

For the first phase: biomass production and CO2 capture, on based the 3A assembly strategy we achieved the first generation with 6 E. coli transformants (Term-OriT, RBS-rbcS, RBS-rbcX, RBS-rbcL, psbAI-RBS, pilA-CA) and the second generation with 3 transformants (RBS-rbcL-Term-OriT, RBS-rbsS-RBS-rbcX, psbAI-RBS-pilA-CA).

This images correspond to PCR results from all constructs that we designed. This confirms that the ligation with the 3A assembly strategy works but it was complicated continuo cloned into ***E. coli***  and the transformation in ***Synechococcus*** sp. because all plasmid ligated into selfs without the BioBrick part.