iGEM 2018 — Team BioIQS-Barcelona

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# Team:BioIQS-Barcelona/Notebook

BIO IQS

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# Wet Lab | Notebook

[Have a look!](https://2018.igem.org/Team%3ABioIQS-Barcelona/Notebook#first-desc)

#### *Over the summer, the BioIQS team has compiled an account of all the work conducted. We believe it will help future iGEM generations to understand and continue our work.*

#### In our project, we followed 3 different experimental lines:

Obtention of the HLA-DQ gene from a DNA sample of a celiac patient.

Synthetic constructs for *E.coli*

Synthetic constructs for *P.pastoris*.

Different members conducted each of these parts. To avoid misunderstandings, we kept track of the work done every week in three different notebooks.

[Download gene extraction notebook](https://static.igem.org/mediawiki/2018/2/2f/T--BioIQS-Barcelona--2018_journal_gene_extraction.pdf)

[Download constructs *E.coli* notebook](https://static.igem.org/mediawiki/2018/5/54/T--BioIQS-Barcelona--2018_journal_constructs_ecoli.pdf)

[Download constructs *P.pastoris* notebook](https://static.igem.org/mediawiki/2018/1/1f/T--BioIQS-Barcelona--2018_journal_pichia_pastoris.pdf)


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# Team:BioIQS-Barcelona/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

This page should contain:

* Protocols
* Experiments
* Documentation of the development of project

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:BioIQS-Barcelona/Results

BIO IQS

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# Project | Results

[Have a look!](https://2018.igem.org/Team%3ABioIQS-Barcelona/Results#first-steps)

## Achievements of our project

# *1.*

#### Computationally [demonstrated](https://2018.igem.org/Team%3ABioIQS-Barcelona/Standardization) that primer binding regions are perfectly conserved or slightly different for both DQ2 and DQ8 celiac haplotypes separately. This means that either the same primer can be used for amplifying all genotypes, or a specific HLA-DQ2- or HLA-DQ8-primer can be designed, reducing the cost of the sensor.

# *2.*

#### Successfully [designed](https://2018.igem.org/Team%3ABioIQS-Barcelona/Basic_Part) a simple and cheap way to extract the extracellular domain of both alpha and beta chains of the HLA-DQ protein from the human genome. This would make the extraction of all HLA-DQ existing variants possible using only one protocol.

# *3.*

#### Thoroughly designed and characterized multiple [composite parts](https://2018.igem.org/Team%3ABioIQS-Barcelona/Composite_Part) to ensure the correct expression of the human HLA-DQ presenting protein in a bacterial or yeast host. This would enable us to determine the best way to produce the elements needed for the sensor.

# *4.*

#### Correctly cloned one of the constructs into the desired expression vector and prepared it for further expression experiments. Cloning is a tedious work and not always results as one would expect so this is an important achievement!

# *5.*

#### Carefully built a [theoretical model](https://2018.igem.org/Team%3ABioIQS-Barcelona/Model) to illustrate the essential detection mechanism of the sensor. Modeling has become one of the most important jobs in this project and have allowed us to better understand the role of the sensor.