iGEM 2011 — Team Nevada

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# Team:Nevada/Notebook

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[Home](https://2011.igem.org/Team%3ANevada)
[Project](https://2011.igem.org/Team%3ANevada/Project)
[Notebook](https://2011.igem.org/Team%3ANevada/Notebook)
[Parts](https://2011.igem.org/Team%3ANevada/Parts)
[Media](https://2011.igem.org/Team%3ANevada/Media)

[Fundraisers](https://2011.igem.org/Team%3ANevada/Foundraiser)
[Gallery](https://2011.igem.org/Team%3ANevada/PhotoGallery)
[Official Site](http://www.igemteamnevada.webs.com)
[Reference](https://2011.igem.org/Team%3ANevada/Reference)

E. Coli
Cyano
Enzymology
Media

Week 1 - June 1-4

-----------------------

E. Coli

Comment Here

Cyano

Comment Here

Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion.

Rxn:* 1. glucose + ATP --Hx-> G-6-P + ADP
* 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH

Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.

Results: Final NADH concentrations were determined using the molar extinction coefficient and were proportionate to initial glucose concentrations.
Discussion: Focus is now to measure not only glucose, but fructose concentrations as well as both with be secreted in the form of sucrose using a D-glucose/D-fructose assay (Megaenzyme.

Media

[Week 1](https://2011.igem.org/Team%3ANevada/Notebook)

[Week 2](https://2011.igem.org/Team%3ANevada/Notebook/June/Week2)

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