iGEM 2017 — Team TU-Eindhoven

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# Team:TU-Eindhoven/Notebook

Untitled Document

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[Members](https://2017.igem.org/Team%3ATU-Eindhoven/Team)
Advisors
Collaborations
Sponsors
Attributions

![](https://static.igem.org/mediawiki/2017/b/b6/T--TU-Eindhoven--Project.png)

Description
Background
Design
Experiments
Timeline
Protocols

![](https://static.igem.org/mediawiki/2017/b/b2/T--TU-Eindhoven--Modeling.png)

![](https://static.igem.org/mediawiki/2017/1/1a/T--TU-Eindhoven--Results.png)

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# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

##### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

##### Inspiration

You can see what others teams have done to organize their notes:

* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)

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# Team:TU-Eindhoven/Experiments

Untitled Document

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[Members](https://2017.igem.org/Team%3ATU-Eindhoven/Team)
Advisors
Collaborations
Sponsors
Attributions

![](https://static.igem.org/mediawiki/2017/b/b6/T--TU-Eindhoven--Project.png)

Description
Background
Design
Experiments
Timeline
Protocols

![](https://static.igem.org/mediawiki/2017/b/b2/T--TU-Eindhoven--Modeling.png)

![](https://static.igem.org/mediawiki/2017/1/1a/T--TU-Eindhoven--Results.png)

![](https://static.igem.org/mediawiki/2017/8/80/T--TU-Eindhoven--Human_Practices.png)

![](https://static.igem.org/mediawiki/2017/4/42/T--TU-Eindhoven--Safety.png)

[![](https://static.igem.org/mediawiki/2017/8/86/T--TU-Eindhoven--JudgingForm.png)](https://igem.org/2017_Judging_Form?team=TU-Eindhoven)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

##### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

##### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

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# Team:TU-Eindhoven/Results

Menu

* [Home](https://2017.igem.org/Team%3ATU-Eindhoven)
* [Project](https://2017.igem.org/Team%3ATU-Eindhoven/Project)
  + [Background](https://2017.igem.org/Team%3ATU-Eindhoven/Project/Background)
  + [Design & Parts](https://2017.igem.org/Team%3ATU-Eindhoven/Project/Design)
  + [Proof of
    Concept](https://2017.igem.org/Team%3ATU-Eindhoven/Demonstrate)
  + [Interlab
    study](https://2017.igem.org/Team%3ATU-Eindhoven/InterLab)
  + [Protocols](https://2017.igem.org/Team%3ATU-Eindhoven/Project/Protocols)
* [Model](https://2017.igem.org/Team%3ATU-Eindhoven/Model)
  + [2D Simulation](https://2017.igem.org/Team%3ATU-Eindhoven/Model/2D_Simulation)
  + [Rule based
    model](https://2017.igem.org/Team%3ATU-Eindhoven/Model/Rule_based_model)
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* [Results](https://2017.igem.org/Team%3ATU-Eindhoven/Results)
  + [DNA Results](https://2017.igem.org/Team%3ATU-Eindhoven/Results/DNA)
  + [Model Results](https://2017.igem.org/Team%3ATU-Eindhoven/Results/Model)
  + [Medal Criteria](https://2017.igem.org/Team%3ATU-Eindhoven/Results/Medal_criteria)
* [Human Practices](https://2017.igem.org/Team%3ATU-Eindhoven/Human_Practices)

  + [Application Scenario](https://2017.igem.org/Team%3ATU-Eindhoven/Applied_Design)
  + [Collaborations](https://2017.igem.org/Team%3ATU-Eindhoven/Collaborations)
  + [Outreach](https://2017.igem.org/Team%3ATU-Eindhoven/Engagement)
  + [Safe by Design](https://2017.igem.org/Team%3ATU-Eindhoven/Human_Practices/Safe_by_Design)
  + [Stakeholders](https://2017.igem.org/Team%3ATU-Eindhoven/Human_Practices/Stakeholders)
* [Safety](https://2017.igem.org/Team%3ATU-Eindhoven/Safety)
* [Team](https://2017.igem.org/Team%3ATU-Eindhoven/Team)
  + [Members](https://2017.igem.org/Team%3ATU-Eindhoven/Team/Members)
  + [Advisors](https://2017.igem.org/Team%3ATU-Eindhoven/Team/Advisors)
  + [Attributions](https://2017.igem.org/Team%3ATU-Eindhoven/Attributions)
  + [Sponsors](https://2017.igem.org/Team%3ATU-Eindhoven/Team/Sponsors)
  + [Notebook](https://2017.igem.org/Team%3ATU-Eindhoven/Team/Notebook)
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* Results

## The TU Eindhoven team of 2017 has won a golden medal and was nominated for Best New Application!

# Overview of generated results

###### During iGEM we generated many different sorts of results. In the beginning of iGEM we mostly worked on getting the right DNA plasmids, by using different methods, like Gibson assembly and Traditional Cloning. In this period, the most important results included agarose gels and sequencing result (they are presented in ["DNA results"](https://2017.igem.org/Team%3ATU-Eindhoven/Results/DNA)). After this period, we had to test the expression of our designed proteins and instead of agarose gels, SDS-PAGE gels became more important. The last period is marked by the "real" experiments, where we could finally test if our designed system indeed worked as planned. To get an overview of all the steps we performed in the lab, go see our ["Notebook"](https://2017.igem.org/Team%3ATU-Eindhoven/Team/Notebook) page. **The results of our Proof of Concept can be found [here.](https://2017.igem.org/Team%3ATU-Eindhoven/Demonstrate)** As iGEM is a broad competition based around synthetic biology, we also did many other things, for example thinking about safety and the future, which are part of ["Human Practices"](https://2017.igem.org/Team%3ATU-Eindhoven/Human_Practices). Another important part was making a model of our system. It took a lot of time to start up, but in the end we got to simulate our system (["Model Results"](https://2017.igem.org/Team%3ATU-Eindhoven/Results/Model)). During our collaboration with Potsdam we discovered that they had no team member with experience with modeling, so we offered to also model their system, the results can be found below our own results, but also on their WIKI page: ["Potsdam"](https://2017.igem.org/Team%3APotsdam/Model)). Additionally, their lack of experience an the time it took for us to start up with the model, inspired us to design a software tool: ["NFsim manual for dummies"](https://2017.igem.org/Team%3ATU-Eindhoven/Software).

# Accomplishments in short

###### * Successfully designed, expressed and characterized multivalent constructs that will gelate after an inducer is added. * Modelled the behavior of GUPPI and made a general tool for simulating other constructs with the same principle. * Contacted stakeholders and integrated their input for a safe and useful design of GUPPI. * Uploaded sequences of parts, characterized expression in the pBAD vector and delivered a codon optimized mCherry part. See our ["Medal Criteria"](https://2017.igem.org/Team%3ATU-Eindhoven/Results/Medal_criteria) page for a broad list of all our accomplishments during iGEM.

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