iGEM 2021 — Team SUNY_Oneonta

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:SUNY Oneonta/Notebook

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2018 Munich](https://2018.igem.org/Team%3AMunich/Notebook)
* [2019 Georgia State](https://2019.igem.org/Team%3AGeorgia_State/Notebook)
* [2019 Newcastle](https://2019.igem.org/Team%3ANewcastle/Notebook)
* [2020 IISER Pune India](https://2020.igem.org/Team%3AIISER-Pune-India/Notebook)
* [2020 Lund](https://2020.igem.org/Team%3ALund/Notebook)
* [2020 NOVA LxPortugal](https://2020.igem.org/Team%3ANOVA_LxPortugal/Notebook)
* [2020 RDFZ China](https://2020.igem.org/Team%3ARDFZ-China/NoteBook)

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:SUNY Oneonta/Engineering

Engineering | iGEM SUNY\_Oneonta

[## SNflaPs](https://2021.igem.org/Team%3ASUNY_Oneonta)

* Project Inspiration

  [Inspiration](https://2021.igem.org/Team%3ASUNY_Oneonta/Inspiration)[Promotional Video](https://2021.igem.org/Team%3ASUNY_Oneonta/Promo_Video)[Presentation](https://2021.igem.org/Team%3ASUNY_Oneonta/Presentation)
* Project

  [Overview](https://2021.igem.org/Team%3ASUNY_Oneonta/Description)[Sample Preparation](https://2021.igem.org/Team%3ASUNY_Oneonta/Sample_Prep)[Detection](https://2021.igem.org/Team%3ASUNY_Oneonta/Detection)[Modeling](https://2021.igem.org/Team%3ASUNY_Oneonta/Model)[Proof-of-Concept](https://2021.igem.org/Team%3ASUNY_Oneonta/Proof_Of_Concept)
* Hardware/Software

  [Hardware](https://2021.igem.org/Team%3ASUNY_Oneonta/Hardware)[Fluorimeter](https://2021.igem.org/Team%3ASUNY_Oneonta/Fluorimeter)[Heat Block](https://2021.igem.org/Team%3ASUNY_Oneonta/Heat_Block)
* Judging

  [Parts](https://2021.igem.org/Team%3ASUNY_Oneonta/Part_Collection)[Implementation](https://2021.igem.org/Team%3ASUNY_Oneonta/Implementation)[Engineering](https://2021.igem.org/Team%3ASUNY_Oneonta/Engineering)[Contributions](https://2021.igem.org/Team%3ASUNY_Oneonta/Contribution)[Medal Criteria](https://2021.igem.org/Team%3ASUNY_Oneonta/Judging)
* Human Practices

  [Engaging Stakeholders](https://2021.igem.org/Team%3ASUNY_Oneonta/Human_Practices)[Collaborations](https://2021.igem.org/Team%3ASUNY_Oneonta/Collaborations)[Educational Outreach](https://2021.igem.org/Team%3ASUNY_Oneonta/Communication)
* Team

  [Team](https://2021.igem.org/Team%3ASUNY_Oneonta/Team)[Attributions](https://2021.igem.org/Team%3ASUNY_Oneonta/Attributions)[Sponsors](https://2021.igem.org/Team%3ASUNY_Oneonta/Sponsors)
* Lab Notebook

  [Protocols](https://2021.igem.org/Team%3ASUNY_Oneonta/Protocols)[Daily Log](https://2021.igem.org/Team%3ASUNY_Oneonta/LabNotebooks)[Safety](https://2021.igem.org/Team%3ASUNY_Oneonta/Safety)

# Engineering

---

##### Contents

In the 2020 iGEM competition, Team SUNY\_Oneonta aimed to create a genotyping system that would detect the A1 and A2 allele with on-site testing. For this competition year, we aimed to accomplish this goal whilst simultaneously expanding on it. Our new goal was to expand our testing system to not only detect the A1 and A2 allele but to detect other single nucleotide polymorphisms as well. With this goal in mind, we took several steps to refine and build upon our genotyping system project. In particular, we improved our heat block design by going through several cycles of data collection and device redesign and we employed information derived from our molecular model in the design criteria of our synthetic DNAs.

# Refinement of the heat block design

* Utilizing information collected the previous competition year (2020), part refinements were made on the original heat block prototype.
* Each part of the prototype was exchanged with different models of the same part and tested for its ability to maintain a stable temperature.
* Parts that produced optimal results were chosen for our final design.

For additional details, see our [Implementation](https://2021.igem.org/Team%3ASUNY_Oneonta/Implementation) page

# Bioinformatics research and development

* Due to the expansion of our project, new targets were identified for our genotyping system to detect. These targets were chosen because they are associated with cattle diseases that can be detrimental when breeding.
* Positive and negative control DNAs were designed and synthesized for each of the new targets.
* Detection oligos were designed for the wild and mutant type genomic sequences of two additional targets.
* Information from the molecular model initially developed in 2020 and refined this year was used in the development of design criteria for the synthetic DNAs used to detect the new targets.

For additional details, see our [Project Overview](https://2021.igem.org/Team%3ASUNY_Oneonta/Description) page

Email: iGEM@oneonta.edu | [suny.oneonta.edu/iGEM](https://suny.oneonta.edu/igem)

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:SUNY Oneonta/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

### What should this page contain?

* Protocols
* Experiments

### Inspiration

* [2019 Nantes](https://2019.igem.org/Team%3ANantes/Experiments)
* [2019 TU Eindhoven](https://2019.igem.org/Team%3ATU_Eindhoven/Experiments)
* [2019 Mingdao](https://2019.igem.org/Team%3AMingdao/Demonstrate)
* [2020 Amsterdam](https://2020.igem.org/Team%3AAmsterdam/Experiments)
* [2020 NCTU Formosa](https://2020.igem.org/Team%3ANCTU_Formosa/Experiments)
* [2020 USAFA](https://2020.igem.org/Team%3AUSAFA/Experiments)

[![](https://static.igem.org/mediawiki/2021/8/8b/HQ_page_logo.jpg)](https://2021.igem.org)

# Team:SUNY Oneonta/Results

# Results

You can describe the results of your project and your future plans here.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2019 Newcastle](https://2019.igem.org/Team%3ANewcastle/Results)
* [2019 Munich](https://2019.igem.org/Team%3AMunich/Results)
* [2019 Tec Chihuahua](https://2019.igem.org/Team%3ATec-Chihuahua/Results)
* [2020 Aalto Helsinki](https://2020.igem.org/Team%3AAalto-Helsinki/Results)
* [2020 GreatBay SCIE](https://2020.igem.org/Team%3AGreatBay_SCIE/Results)
* [2020 Queens Canada](https://2020.igem.org/Team%3AQueens_Canada/Results)