iGEM 2018 — Team Paris_Bettencourt

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# Team:Paris Bettencourt/Notebook

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[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3AParis_Bettencourt)
[HOME](https://2018.igem.org/Team%3AParis_Bettencourt)

TEAM

[Team Members](https://2018.igem.org/Team%3AParis_Bettencourt/Team)
[Collaborations](https://2018.igem.org/Team%3AParis_Bettencourt/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AParis_Bettencourt/Description)
[Design](https://2018.igem.org/Team%3AParis_Bettencourt/Design)
[Experiments](https://2018.igem.org/Team%3AParis_Bettencourt/Experiments)
[Notebook](https://2018.igem.org/Team%3AParis_Bettencourt/Notebook)
[InterLab](https://2018.igem.org/Team%3AParis_Bettencourt/InterLab)
[Model](https://2018.igem.org/Team%3AParis_Bettencourt/Model)
[Results](https://2018.igem.org/Team%3AParis_Bettencourt/Results)
[Demonstrate](https://2018.igem.org/Team%3AParis_Bettencourt/Demonstrate)
[Improve](https://2018.igem.org/Team%3AParis_Bettencourt/Improve)
[Attributions](https://2018.igem.org/Team%3AParis_Bettencourt/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AParis_Bettencourt/Parts)
[Basic Parts](https://2018.igem.org/Team%3AParis_Bettencourt/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AParis_Bettencourt/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AParis_Bettencourt/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AParis_Bettencourt/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AParis_Bettencourt/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AParis_Bettencourt/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AParis_Bettencourt/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AParis_Bettencourt/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AParis_Bettencourt/Hardware)
[Measurement](https://2018.igem.org/Team%3AParis_Bettencourt/Measurement)
[Model](https://2018.igem.org/Team%3AParis_Bettencourt/Model)
[Plant](https://2018.igem.org/Team%3AParis_Bettencourt/Plant)
[Software](https://2018.igem.org/Team%3AParis_Bettencourt/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=Paris_Bettencourt)

# Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

### What should this page have?

* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

### Inspiration

You can see what others teams have done to organize their notes:

* [2014 ATOMS-Turkiye](https://2014.igem.org/Team%3AATOMS-Turkiye/Notebook)
* [2014 Tec Monterrey](https://2014.igem.org/Team%3ATec-Monterrey/ITESM14_project.html#tab_notebook)
* [2014 Kyoto](https://2014.igem.org/Team%3AKyoto/Notebook/Magnetosome_Formation#title)
* [2014 Cornell](https://2014.igem.org/Team%3ACornell/notebook)


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:Paris Bettencourt/Experiments

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[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3AParis_Bettencourt)
[HOME](https://2018.igem.org/Team%3AParis_Bettencourt)

TEAM

[Team Members](https://2018.igem.org/Team%3AParis_Bettencourt/Team)
[Collaborations](https://2018.igem.org/Team%3AParis_Bettencourt/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3AParis_Bettencourt/Description)
[Design](https://2018.igem.org/Team%3AParis_Bettencourt/Design)
[Experiments](https://2018.igem.org/Team%3AParis_Bettencourt/Experiments)
[Notebook](https://2018.igem.org/Team%3AParis_Bettencourt/Notebook)
[InterLab](https://2018.igem.org/Team%3AParis_Bettencourt/InterLab)
[Model](https://2018.igem.org/Team%3AParis_Bettencourt/Model)
[Results](https://2018.igem.org/Team%3AParis_Bettencourt/Results)
[Demonstrate](https://2018.igem.org/Team%3AParis_Bettencourt/Demonstrate)
[Improve](https://2018.igem.org/Team%3AParis_Bettencourt/Improve)
[Attributions](https://2018.igem.org/Team%3AParis_Bettencourt/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3AParis_Bettencourt/Parts)
[Basic Parts](https://2018.igem.org/Team%3AParis_Bettencourt/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3AParis_Bettencourt/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3AParis_Bettencourt/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3AParis_Bettencourt/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3AParis_Bettencourt/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3AParis_Bettencourt/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3AParis_Bettencourt/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3AParis_Bettencourt/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3AParis_Bettencourt/Hardware)
[Measurement](https://2018.igem.org/Team%3AParis_Bettencourt/Measurement)
[Model](https://2018.igem.org/Team%3AParis_Bettencourt/Model)
[Plant](https://2018.igem.org/Team%3AParis_Bettencourt/Plant)
[Software](https://2018.igem.org/Team%3AParis_Bettencourt/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=Paris_Bettencourt)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:Paris Bettencourt/Results

[![](https://static.igem.org/mediawiki/2018/6/63/T--Paris_Bettencourt--STARCORE_logo.png)](https://2018.igem.org/Team%3AParis_Bettencourt)

* Project
  + [Human Practices](https://2018.igem.org/Team%3AParis_Bettencourt/Human_Practices)
  + [Applied Design](https://2018.igem.org/Team%3AParis_Bettencourt/Applied_Design)
  + [Design](https://2018.igem.org/Team%3AParis_Bettencourt/Design)
  + [Production](https://2018.igem.org/Team%3AParis_Bettencourt/Production)
  + [Testing](https://2018.igem.org/Team%3AParis_Bettencourt/Active_Testing)
    - [Active Testing](https://2018.igem.org/Team%3AParis_Bettencourt/Active_Testing)
    - [Biocompatibility Testing](https://2018.igem.org/Team%3AParis_Bettencourt/Biocompatibility_Testing)+ [Modeling](https://2018.igem.org/Team%3AParis_Bettencourt/Model)
    + [Optimization](https://2018.igem.org/Team%3AParis_Bettencourt/Optimization)
    + [Public Engagement](https://2018.igem.org/Team%3AParis_Bettencourt/Public_Engagement)
* [Software](https://2018.igem.org/Team%3AParis_Bettencourt/Software)
  + [AMP Designer Overview](https://2018.igem.org/Team%3AParis_Bettencourt/AMPDesigner_Overview)
  + [AMP Forest](https://2018.igem.org/Team%3AParis_Bettencourt/AMP_Forest)
  + [AMP Evolver](https://2018.igem.org/Team%3AParis_Bettencourt/AMP_Evolver)
  + [Result](https://2018.igem.org/Team%3AParis_Bettencourt/Result)
* Judging
  + [Medal Criteria](https://2018.igem.org/Team%3AParis_Bettencourt/Medal_Criteria)
  + [Key Results](https://2018.igem.org/Team%3AParis_Bettencourt/Results)
  + [BioBricks](https://2018.igem.org/Team%3AParis_Bettencourt/BioBricks)
  + [InterLab](https://2018.igem.org/Team%3AParis_Bettencourt/InterLab)* Team
    + [Members](https://2018.igem.org/Team%3AParis_Bettencourt/Members)
    + [Collaborations](https://2018.igem.org/Team%3AParis_Bettencourt/Collaborations)
    + [Attributions](https://2018.igem.org/Team%3AParis_Bettencourt/Attributions)
  * [Lab Notebook](https://2018.igem.org/Team%3AParis_Bettencourt/Lab_Notebook)
  * [Acknowledgements](https://2018.igem.org/Team%3AParis_Bettencourt/Acknowledgements)

  [![](https://static.igem.org/mediawiki/2017/f/fd/Logo-iGEMPB.png)](https://2018.igem.org/Main_Page "iGEM 2018")

  [@iGEM\_Paris](https://twitter.com/iGEM_Paris)

![](https://static.igem.org/mediawiki/2018/d/d5/T--Paris_Bettencourt--STARCORE_logoBW.png)

![](https://static.igem.org/mediawiki/2018/7/77/T--Paris_Bettencourt--Trophee_J.png)

# Key Results

## Key results from the Human Practices

- By talking to experts, farmers and veterinaries, we identified that antimicrobial resistance of coliforms affecting piglets is a major concern - We obtained access to raw data from ANSES and found out that this data highlights amoxicillin and tetracycline as drugs with the most frequent evolved resistance |

## Key results from the STAR CORE Design and Production

- We successfully cloned 131 STAR CORE combinations. - 9 out of the 11 proteins we expressed exhibited antibacterial activity comparable to existing antibiotics and AMPs. |

## Key results from the Testing

- The STAR CORE we have obtained show antimicrobial activity. - 8 out of the 10 constructs showed antimicrobial activity towards E. coli while 9 out of the 10 constructs displayed antimicrobial potential towards B. subtilis. - The top performing STAR CORE was the Ferritin-Alyteserin fusion, with an activity almost 10 times higher than that of the control.   Some StarCores exhibited species-specific killing, being particularly active against E. coli. - StarCores of varying geometry displayed similar MIC values. - There was no clear correlation between the geometry of the STAR CORE and their antimicrobial activity, indicating a non-specific mechanism of action. |

## Key results from Optimization and Modeling

- Decreasing net charge on the peptide improves expression efficiency, however it decreases its antibacterial activity |

[![](https://static.igem.org/mediawiki/2018/7/7e/T--Paris_Bettencourt--PBlogo_Blanche.png)](https://2018.igem.org/Team%3AParis_Bettencourt)

**Centre for Research and Interdisciplinarity (CRI)**
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
![](https://static.igem.org/mediawiki/2017/7/74/PB_footeremail.png)

[![](https://static.igem.org/mediawiki/2018/d/da/T--Paris_Bettencourt--Fondation_Bettencourt.png)](http://www.fondationbs.org/)

[![](https://static.igem.org/mediawiki/2018/1/12/T--Paris_Bettencourt--Logo_CRI.png)](https://cri-paris.org/)

[![](https://static.igem.org/mediawiki/2018/b/b5/T--Paris_Bettencourt--iGEM_logo.png)](https://2018.igem.org/Main_Page)