iGEM 2014 — Team TCU_Taiwan

[![](https://2014.igem.org/images/wiki.png)"](/Main_Page "Main Page")

* [Page](/Team%3ATCU_Taiwan/Notebook)
* [Discussion](/wiki/index.php?title=Talk:Team:TCU_Taiwan/Notebook&action=edit&redlink=1)
* [View source](/wiki/index.php?title=Team:TCU_Taiwan/Notebook&action=edit)
* [History](/wiki/index.php?title=Team:TCU_Taiwan/Notebook&action=history)
* teams

* [Log in](/wiki/index.php?title=Special:UserLogin&returnto=Team:TCU_Taiwan/Notebook "You are encouraged to log in; however, it is not mandatory [o]")

# Team:TCU Taiwan/Notebook

### From 2014.igem.org

|  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- |
| |  |  |  |  |  | | --- | --- | --- | --- | --- | |  | ![](https://static.igem.org/mediawiki/2014/2/25/TCU-Notebook-logo.png) | Notebook | ![](https://static.igem.org/mediawiki/2014/c/c2/TCU-Logo-up.png) |  |  |  |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | --- | | * [Home](https://2014.igem.org/Team%3ATCU_Taiwan) | * Team   + [Members](https://2014.igem.org/Team%3ATCU_Taiwan/Team)   + [Achievements](https://2014.igem.org/Team%3ATCU_Taiwan/Achievements)   + [Official Team Profile](https://igem.org/Team.cgi?id=1473)   + [Contact](https://2014.igem.org/Team%3ATCU_Taiwan/Contact) | * Project   + [Introduction](https://2014.igem.org/Team%3ATCU_Taiwan/Project)   + [Parts](https://2014.igem.org/Team%3ATCU_Taiwan/Parts)   + [System Design](https://2014.igem.org/Team%3ATCU_Taiwan/SystemDesign)   + [M13 Phage](https://2014.igem.org/Team%3ATCU_Taiwan/M13Phage)   + [CRISPR](https://2014.igem.org/Team%3ATCU_Taiwan/CRISPR)   + [Future Works](https://2014.igem.org/Team%3ATCU_Taiwan/FutureWorks)   + [Human Pratics](https://2014.igem.org/Team%3ATCU_Taiwan/HumanPratics) | * [Modeling](https://2014.igem.org/Team%3ATCU_Taiwan/Modeling) | * [Safety](https://2014.igem.org/Team%3ATCU_Taiwan/Safety) | * [Notebook](https://2014.igem.org/Team%3ATCU_Taiwan/Notebook) | * [Attributions](https://2014.igem.org/Team%3ATCU_Taiwan/Attributions) | * [![](https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png)](https://2014.igem.org/Main_Page) |  | ![](https://static.igem.org/mediawiki/2014/c/c1/TCU-Line.png) | | | |    |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | | |  |  |  | | --- | --- | --- | |  | | | | iGEM materials | | | |  | | | | Bacteria medium   \*LB(Luria-Bertani) broth: 1% Trytone, 1% NaCl, 0.5% Yeast extract in the ddH2O  \*LB agar plate:LB broth add 1.5% agar.If you want to add selection marker example for ampicillin or chloramphenicol. To culture ***E.coli***, ampicillin is added 100 mg/ml for usage, chloramphenicol is added 30 mg/ml for usage, kanamycin is added 70 mg/ml for usage | | | |  | | | | Plasmid isolation  \*Solution I: 50 mM glucose、25 mM Tris-HCl (pH 8.0)、10 mM EDTA  \*Solution II:0.2N NaOH and 1% SDS(Sodium dodecyl sulfate ) mix  \*Solution III: 5 M potassium acetate (pH4.8), 11.5% glacial acetic acid  \*RNase A(10 mg/ml)  \*Phenol chloroform  \*Chloroform  \*95% Ethanol  \*Tris-EDTA(TE)buffer:10 mM Tris-Cl(pH 7.5) 1 mM EDTA  \*pBluescript SK(-)  \*BBa\_K1218011  \*BBa\_K914003  \*BBa\_I13521 | | | |  | | | | Quick screening  \*Quick screening buffer(Tris-Cl pH 12.6, 3% SDS)  \*Phenol chloroform  \*Chloroform | | | |  | | | | Enzyme digestion   \*T4 ligase  \*10X ligation buffer  \**Eco*RI  \**Pst*I  \**Spe*I  \**Xba*I  \*BSA 0.1% | | | |  | | | | Bacteria strains and others  \*DH5a  \*JM101  \*0.8% and 2% agarose gel  \*95% Ethanol  \*6X loading dye  \*1.5 ml centrifuge tube  \*250 ml flask  \*M13KO7(1\*1011 pfu/ml)from *NEW ENGLAND BioLabs* | | | |  | | | | Polymerase chain reaction  \**Top* DNA polymerase  \*dNTP(dATP, dCTP, dGTP, dTTP) 250 mM  \*Tris-HCl(pH9.0) 10 mM  \*KCl 30 mM  \*MgCl2 1.5 mM | | | |  | | | | Instruments  \*VCM-620, laminal flow  \*Biofuge pico, Heraeus  \* OS1500-H, TSK Oribital Shaking Iucubator  \* Biowave  \* Vortex-GENE 2  \* Autoclave AS-1060L  \* GenAmp PCR System 2400, Applied Biosystems  \*BU-420, YIHDER  \*5804R, eppendorf Centrifuge | | | |  | | | |  | | | | Life Records | | | |  |  |  | | |  | |  | |  | |  | | |  | | --- | |  | | Study Together | |  | |  | |  |  |  | | --- | |  | | Conference in NCTU | |  | |  | |  | | |  | | --- | | Trip in Chihsingtan Beach | |  | |  | |  | |  | | |  | |  |  | | --- | | ![](https://static.igem.org/mediawiki/2014/c/c1/TCU-Line.png) |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | | ^ [![](https://static.igem.org/mediawiki/2014/8/84/TCU-Contact-bot.png)](https://2014.igem.org/Team%3ATCU_Taiwan/Contact "Contact Us")    ![](https://static.igem.org/mediawiki/2014/c/c4/TCU%E3%84%A6Gmail.png)  [![](https://static.igem.org/mediawiki/2014/b/b9/TCU-Facebook.png)](https://www.facebook.com/TCUiGEM?ref=ts&fref=ts "Facebook") [![](https://static.igem.org/mediawiki/2014/2/29/TCU_Youtube.png)](https://www.youtube.com/channel/UCr40ZmaFNJGx7tUufCXK_OQ "YouTube") [![](https://static.igem.org/mediawiki/2014/f/f0/TCU-Wordpress.png)](http://tcuigem.wordpress.com/ "Wordpress") |  | |  |  |  |  |  |  |  |  | | --- | --- | --- | --- | --- | --- | --- | --- | | Team Members | Project | Parts | Human Pratics | Modeling | Safety | Notebook | Attributions | | [![](https://static.igem.org/mediawiki/2014/1/1a/TCU-Team-logo.png)](https://2014.igem.org/Team%3ATCU_Taiwan/Team "Team Members") | [![](https://static.igem.org/mediawiki/2014/f/f4/TCU-Project-logo.png)](https://2014.igem.org/Team%3ATCU_Taiwan/Project "Project") | [![](https://static.igem.org/mediawiki/2014/e/e5/TCU-Part-logo.png)](https://2014.igem.org/Team%3ATCU_Taiwan/Parts "Parts") | [![](https://static.igem.org/mediawiki/2014/d/dd/TCU-Human-logo.png)](https://2014.igem.org/Team%3ATCU_Taiwan/HumanPratics "Human Pratics") | [![](https://static.igem.org/mediawiki/2014/3/3f/TCU-Modeling-logo.png)](https://2014.igem.org/Team%3ATCU_Taiwan/Modeling "Modeling") | [![](https://static.igem.org/mediawiki/2014/2/21/TCU-Safety-logo.png)](https://2014.igem.org/Team%3ATCU_Taiwan/Safety "Safety") | [![](https://static.igem.org/mediawiki/2014/2/25/TCU-Notebook-logo.png)](https://2014.igem.org/Team%3ATCU_Taiwan/Notebook "Notebook") | [![](https://static.igem.org/mediawiki/2014/5/51/TCU-Attributions-logo.png)](https://2014.igem.org/Team%3ATCU_Taiwan/Attributions "Attributions") |   Lost the way? Use it to help you if you're lost. |  | [![](https://static.igem.org/mediawiki/2014/c/c3/TCU-Logo-d.png)](https://2014.igem.org/Team%3ATCU_Taiwan "Home")  ![](http://i.creativecommons.org/l/by/3.0/88x31.png) |   ^ | | |

Retrieved from "[http://2014.igem.org/Team:TCU\_Taiwan/Notebook](http://2014.igem.org/Team%3ATCU_Taiwan/Notebook)"

[![Powered by MediaWiki](/wiki/skins/common/images/poweredby_mediawiki_88x31.png)](https://www.mediawiki.org/)

[![Attribution 3.0 Unported](https://i.creativecommons.org/l/by/3.0/88x31.png)](https://creativecommons.org/licenses/by/3.0/)

* [Recent changes](/Special%3ARecentChanges "Recent changes")
* [What links here](/Special%3AWhatLinksHere/Team%3ATCU_Taiwan/Notebook "List of all wiki pages that link here [j]")
* [Related changes](/Special%3ARecentChangesLinked/Team%3ATCU_Taiwan/Notebook "Recent changes in pages linked from this page [k]")
* [Special pages](/Special%3ASpecialPages "List of all special pages [q]")
* [My preferences](/Special%3APreferences)

* [Printable version](/wiki/index.php?title=Team:TCU_Taiwan/Notebook&printable=yes "Printable version of this page [p]")
* [Permanent link](/wiki/index.php?title=Team:TCU_Taiwan/Notebook&oldid=340107 "Permanent link to this revision of the page")
* [Privacy policy](/2014.igem.org%3APrivacy_policy "2014.igem.org:Privacy policy")
* [Disclaimers](/2014.igem.org%3AGeneral_disclaimer "2014.igem.org:General disclaimer")