iGEM 2015 — Team San_Andres

# Team:San Andres/Notebook

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![File:Gluten-s-Job.jpeg](https://static.igem.org/mediawiki/2015/thumb/8/8b/Gluten-s-Job.jpeg/800px-Gluten-s-Job.jpeg)

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| [Home](https://2015.igem.org/Team%3ASan_Andres) | [Team](https://2015.igem.org/Team%3ASan_Andres/Team) | [Project](https://2015.igem.org/Team%3ASan_Andres/Description) | [Parts](https://2015.igem.org/Team%3ASan_Andres/Parts) | [Modeling](https://2015.igem.org/Team%3ASan_Andres/Modeling) | [Results](https://2015.igem.org/Team%3ASan_Andres/Results) |

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| [Notebook](https://2015.igem.org/Team%3ASan_Andres/Notebook) | [Human Practices](https://2015.igem.org/Team%3ASan_Andres/Practices) | [Future Projections](https://2015.igem.org/Team%3ASan_Andres/Design) | [Attributions](https://2015.igem.org/Team%3ASan_Andres/Attributions) | [Collaborations](https://2015.igem.org/Team%3ASan_Andres/Collaborations) |

# Notebook

This
project was initiated in April of the year 2014 and in this time we
have
acquired new experiences like to know students from other schools and
communes
with our same interests, also with our same age range, which goes
promoting
inter-school knowledges. Also this project has served as a guide for
the
future, helping us to understand University life through various clases
of the
sience fields, as in the case of molecular biology, where it taught us
the
basics of this, which helped us to understand concepts like what is an
plasmid?, and how use it, things which helped us to generate an idea of
how to
solve a problem. Then
we learned various techniques from laboratories to handle the
bacterias,stuff of laboratory, laboratory's machines and biobricks,
essensial
things for our work, and like that start own project, in this case
about celiac
disease.

![File:Lab 800.jpg](https://static.igem.org/mediawiki/2015/0/01/Lab_800.jpg)
![File:Bio molecular.png](https://static.igem.org/mediawiki/2015/3/39/Bio_molecular.png)

Lab (2015)

* January:
  Cultivation of Biobricks, extraction plasmidial and double digestion.

![Biobricks.jpg](https://static.igem.org/mediawiki/2015/thumb/c/c5/Biobricks.jpg/300px-Biobricks.jpg) ![https://static.igem.org/mediawiki/2015/thumb/7/70/Electro1.jpg/800px-Electro1.jpg](https://static.igem.org/mediawiki/2015/thumb/7/70/Electro1.jpg/800px-Electro1.jpg)

![File:Tabla.png](https://static.igem.org/mediawiki/2015/e/ee/Tabla.png)

* February:
  Preparation of methodologies and ligation of KumaMax with RFP.

![File:Electro2jpg.png](https://static.igem.org/mediawiki/2015/e/e2/Electro2jpg.png) ![File:Methodology.jpg](https://static.igem.org/mediawiki/2015/thumb/3/34/Methodology.jpg/800px-Methodology.jpg)

* March:
  Presentation of the project to CORFO.

![File:120315 IGEM 154 copia.jpg](https://static.igem.org/mediawiki/2015/f/f3/120315_IGEM_154_copia.jpg)
![File:Igem marzo 2015.jpg](https://static.igem.org/mediawiki/2015/thumb/8/8f/Igem_marzo_2015.jpg/800px-Igem_marzo_2015.jpg)

* May:
  Samples are lost. Restart procedures.

![File:Electro3.png](https://static.igem.org/mediawiki/2015/thumb/c/c8/Electro3.png/800px-Electro3.png)
![File:Biobricks2.jpg](https://static.igem.org/mediawiki/2015/e/ee/Biobricks2.jpg)

* June:
  Double digestion and links which fail.

![File:Electro4.png](https://static.igem.org/mediawiki/2015/5/57/Electro4.png) ![File:Electro5.jpg](https://static.igem.org/mediawiki/2015/b/bf/Electro5.jpg)

* July:
  Apply plan of ligations.

![File:Electro6.jpg](https://static.igem.org/mediawiki/2015/7/79/Electro6.jpg)
 ![https://static.igem.org/mediawiki/2015/2/2c/Plan.jpg](https://static.igem.org/mediawiki/2015/2/2c/Plan.jpg)

* August:
  All failed again. Change in methodology for the assambly standar.
  Restart procedures.

![File:Electro9.jpg](https://static.igem.org/mediawiki/2015/thumb/1/1c/Electro9.jpg/800px-Electro9.jpg)
 ![https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg](https://static.igem.org/mediawiki/2015/c/c8/Metodology_2.jpg)

* September:
  Finally works the KMX - RFP ligation and the plasmid.

![File:Electro8.jpg](https://static.igem.org/mediawiki/2015/thumb/4/4f/Electro8.jpg/793px-Electro8.jpg)
![File:Circulo.png](https://static.igem.org/mediawiki/2015/thumb/1/18/Circulo.png/714px-Circulo.png)

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