iGEM 2023 — Team concordia-montreal

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{% block title %}Notebook{% endblock %}
{% block lead %}Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.{% endblock %}
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## What should this page contain?

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* Chronological notes of what your team is doing.
* Brief descriptions of daily important events.
* Pictures of your progress.
* Mention who participated in what task.

## Inspirations

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* [2018 Munich](http://2018.igem.org/Team%3AMunich/Notebook)
* [2019 Georgia State](https://2019.igem.org/Team%3AGeorgia_State/Notebook)
* [2019 Newcastle](https://2019.igem.org/Team%3ANewcastle/Notebook)
* [2020 IISER Pune India](https://2020.igem.org/Team%3AIISER-Pune-India/Notebook)
* [2020 Lund](https://2020.igem.org/Team%3ALund/Notebook)
* [2020 NOVA LxPortugal](https://2020.igem.org/Team%3ANOVA_LxPortugal/Notebook)
* [2020 RDFZ China](https://2020.igem.org/Team%3ARDFZ-China/NoteBook)

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{% block title %}Engineering Success{% endblock %}
{% block lead %}Demonstrate engineering success in a part of your project by going through at least one iteration of the engineering design cycle.{% endblock %}
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#### Silver Medal Criterion #1

Demonstrate engineering success in a part of your project by going through at least one iteration of the engineering design cycle. This achievement should be distinct from your Contribution for Bronze.

If you plan to show engineering success by creating a new Part that has been shown to work as expected, you must document your contribution on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page) for your team to be eligible for this criteria.

---

Please see the [2023 Medals Page](https://competition.igem.org/judging/medals) for more information.

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{% block title %}Experiments{% endblock %}
{% block lead %}Describe the research, experiments, and protocols you used in your iGEM project.{% endblock %}
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## What should this page contain?

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Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

If you made Parts this year, please remember to put all information, characterization, and measurement data on the Part's Main Page on the [Registry](http://parts.igem.org/Main_Page).

## Inspirations

---

* [2019 Nantes](https://2019.igem.org/Team%3ANantes/Experiments)
* [2019 TU Eindhoven](https://2019.igem.org/Team%3ATU_Eindhoven/Experiments)
* [2019 Mingdao](https://2019.igem.org/Team%3AMingdao/Demonstrate)
* [2020 Amsterdam](https://2020.igem.org/Team%3AAmsterdam/Experiments)
* [2020 NCTU Formosa](https://2020.igem.org/Team%3ANCTU_Formosa/Experiments)
* [2020 USAFA](https://2020.igem.org/Team%3AUSAFA/Experiments)

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* Overview
* Process
* Process1
* Process2
* Process3

### Standard gold nanoparticles 40nm

##### 1.1 Covalent conjugation of IgG anti rat in goat 1mg/mL to standard gold nanoparticles

Our standard gold nanoparticles 40nm at 1OD was activated into EDC/NHS buffer to create the thioester bond as a covalent bond on the gold nanoparticles surface to conjugate with antibody IgG anti rat in goat.
However, there was a transparently colorless solution, which could be understood that the buffer did wash away the gold nanoparticles, which left the clear solution
![](https://static.igem.wiki/teams/4684/wiki/Results1.png)

Fig.1 - The UV-vis result of functionalized standard gold nanoparticles with IgG

![](https://static.igem.wiki/teams/4684/wiki/Results2.png)

Fig.2 - The UV-vis result of unfunctionalized standard gold nanoparticles

The UV-vis result of unfunctionalized standard gold nanoparticles showed that the peak of it appeared at wavelength 525 nm in figure 2. However, for the functionalized standard gold nanoparticles with IgG did not show any peak from the range of 520 nm to 540 nm in figure 1, which can be understood that there was no appearance of gold nanoparticles in the solution to conjugate with IgG
Besides that, we also found out that the procedure we used was not suitable for standard gold nanoparticles; it was supposed to be used for carboxyl gold nanoparticles instead with the concentration of IgG had to be 0.5mg/mL for standard gold nanoparticles 40nm.

### Process

Curabitur ut interdum nulla. Nam ligula massa, efficitur vel lectus id, fermentum molestie lacus. Morbi posuere, sapien eu cursus interdum, nibh ante tristique dolor, ac sagittis nibh massa sit amet nisl. Proin eu risus dictum, viverra augue in, elementum arcu. Donec ut leo felis. Nulla pharetra tristique dui, quis fermentum nibh dictum et. Fusce laoreet lectus non nisl laoreet rutrum. Donec cursus diam quis augue aliquet, vel luctus ante posuere. Sed vel molestie magna. Mauris nec tortor commodo, accumsan ante et, gravida dui. Donec cursus urna nec arcu venenatis, et iaculis lacus pretium. Nunc placerat tortor lacus. In aliquam consectetur urna, eu ullamcorper ex mollis non. Integer quis est ac lacus tempor eleifend non id nunc.
Sed non urna quis ante ullamcorper ultrices. Donec placerat rhoncus dapibus. Duis nec mi arcu. Nam gravida pharetra risus. Integer sit amet felis efficitur, laoreet dolor ut, auctor massa. Morbi laoreet, augue fringilla tincidunt varius, elit nisl convallis libero, quis congue enim massa in nunc. Suspendisse ante felis, efficitur tincidunt maximus ut, commodo sed tortor. Nunc quis lacinia augue. Integer leo enim, finibus eget nulla eget, efficitur fermentum nisl. Vestibulum quis commodo velit. Nullam vitae mauris et turpis dictum tincidunt vitae blandit augue. Nam pulvinar maximus neque et tincidunt.

### Process1

Curabitur ut interdum nulla. Nam ligula massa, efficitur vel lectus id, fermentum molestie lacus. Morbi posuere, sapien eu cursus interdum, nibh ante tristique dolor, ac sagittis nibh massa sit amet nisl. Proin eu risus dictum, viverra augue in, elementum arcu. Donec ut leo felis. Nulla pharetra tristique dui, quis fermentum nibh dictum et. Fusce laoreet lectus non nisl laoreet rutrum. Donec cursus diam quis augue aliquet, vel luctus ante posuere. Sed vel molestie magna. Mauris nec tortor commodo, accumsan ante et, gravida dui. Donec cursus urna nec arcu venenatis, et iaculis lacus pretium. Nunc placerat tortor lacus. In aliquam consectetur urna, eu ullamcorper ex mollis non. Integer quis est ac lacus tempor eleifend non id nunc.
Sed non urna quis ante ullamcorper ultrices. Donec placerat rhoncus dapibus. Duis nec mi arcu. Nam gravida pharetra risus. Integer sit amet felis efficitur, laoreet dolor ut, auctor massa. Morbi laoreet, augue fringilla tincidunt varius, elit nisl convallis libero, quis congue enim massa in nunc. Suspendisse ante felis, efficitur tincidunt maximus ut, commodo sed tortor. Nunc quis lacinia augue. Integer leo enim, finibus eget nulla eget, efficitur fermentum nisl. Vestibulum quis commodo velit. Nullam vitae mauris et turpis dictum tincidunt vitae blandit augue. Nam pulvinar maximus neque et tincidunt.

### Process2

Curabitur ut interdum nulla. Nam ligula massa, efficitur vel lectus id, fermentum molestie lacus. Morbi posuere, sapien eu cursus interdum, nibh ante tristique dolor, ac sagittis nibh massa sit amet nisl. Proin eu risus dictum, viverra augue in, elementum arcu. Donec ut leo felis. Nulla pharetra tristique dui, quis fermentum nibh dictum et. Fusce laoreet lectus non nisl laoreet rutrum. Donec cursus diam quis augue aliquet, vel luctus ante posuere. Sed vel molestie magna. Mauris nec tortor commodo, accumsan ante et, gravida dui. Donec cursus urna nec arcu venenatis, et iaculis lacus pretium. Nunc placerat tortor lacus. In aliquam consectetur urna, eu ullamcorper ex mollis non. Integer quis est ac lacus tempor eleifend non id nunc.
Sed non urna quis ante ullamcorper ultrices. Donec placerat rhoncus dapibus. Duis nec mi arcu. Nam gravida pharetra risus. Integer sit amet felis efficitur, laoreet dolor ut, auctor massa. Morbi laoreet, augue fringilla tincidunt varius, elit nisl convallis libero, quis congue enim massa in nunc. Suspendisse ante felis, efficitur tincidunt maximus ut, commodo sed tortor. Nunc quis lacinia augue. Integer leo enim, finibus eget nulla eget, efficitur fermentum nisl. Vestibulum quis commodo velit. Nullam vitae mauris et turpis dictum tincidunt vitae blandit augue. Nam pulvinar maximus neque et tincidunt.

### Process3

Curabitur ut interdum nulla. Nam ligula massa, efficitur vel lectus id, fermentum molestie lacus. Morbi posuere, sapien eu cursus interdum, nibh ante tristique dolor, ac sagittis nibh massa sit amet nisl. Proin eu risus dictum, viverra augue in, elementum arcu. Donec ut leo felis. Nulla pharetra tristique dui, quis fermentum nibh dictum et. Fusce laoreet lectus non nisl laoreet rutrum. Donec cursus diam quis augue aliquet, vel luctus ante posuere. Sed vel molestie magna. Mauris nec tortor commodo, accumsan ante et, gravida dui. Donec cursus urna nec arcu venenatis, et iaculis lacus pretium. Nunc placerat tortor lacus. In aliquam consectetur urna, eu ullamcorper ex mollis non. Integer quis est ac lacus tempor eleifend non id nunc.
Sed non urna quis ante ullamcorper ultrices. Donec placerat rhoncus dapibus. Duis nec mi arcu. Nam gravida pharetra risus. Integer sit amet felis efficitur, laoreet dolor ut, auctor massa. Morbi laoreet, augue fringilla tincidunt varius, elit nisl convallis libero, quis congue enim massa in nunc. Suspendisse ante felis, efficitur tincidunt maximus ut, commodo sed tortor. Nunc quis lacinia augue. Integer leo enim, finibus eget nulla eget, efficitur fermentum nisl. Vestibulum quis commodo velit. Nullam vitae mauris et turpis dictum tincidunt vitae blandit augue. Nam pulvinar maximus neque et tincidunt.

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