iGEM 2019 — Team Sheffield

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# Team:Sheffield/Notebook

* [![](https://static.igem.org/mediawiki/2019/f/f2/T--Sheffield--white_main_logo.png)](https://2019.igem.org/Team%3ASheffield)
* OPENLUX
* project

  [Master Plan](https://2019.igem.org/Team%3ASheffield/Description)
  [hardware](https://2019.igem.org/Team%3ASheffield/Hardware)
  [software](https://2019.igem.org/Team%3ASheffield/Software)
  [modelling](https://2019.igem.org/Team%3ASheffield/Model)
  [demonstrate](https://2019.igem.org/Team%3ASheffield/Demonstrate)
* [human practices](https://2019.igem.org/Team%3ASheffield/Human_Practices)
* [team](https://2019.igem.org/Team%3ASheffield/Team)
* [notebook](https://2019.igem.org/Team%3ASheffield/Notebook)
* [judging](https://2019.igem.org/Team%3ASheffield/Judging)

progress during our iGEM project.

## Week 1

24 june 2019

**biology**

Brainstorming for possible projects.

Literature research.

Finalising project choice.

**Designing the device**

Decided to improve upon the cheap DIY plate reader started by the 2017 Sheffield Igem project, Bright biotics.

**engineering**

Research into the previous project on plate readers(Bright Biotics).

Viewing of CAD files from Bright Biotics.

Interview with an engineer from the Bright Biotics project.

Ordering of items for building new plate reader.

**wiki and media design**

Brainstorming on design concept for our wiki.

[Read more](https://static.igem.org/mediawiki/2019/4/4f/T--Sheffield--notebook_week1.pdf)

## Week 2

05 July 2019

**biology**

Orientation and safety sessions in the wet lab.

Research into applications of plate readers in biology.

Research into choice of bacterial strains for testing the plate reader.

**designing a device**
 Made a list of the areas to improve upon and started modelling the new device.

**engineering**

Orientation and safety sessions in the makerspace (iFORGE).

Research into ways to improve upon previous plate reader project.

3D printing of moulds, testing sensors and working on the LED matrix for the new plate reader.

**wiki and media design**
Deciding a template for the wiki and the main sections.

[Read more](https://static.igem.org/mediawiki/2019/7/79/T--Sheffield--notebook_week2.pdf)

## Week 3

12 July 2019

**biology**
Shortlisting the different assays for which we will employ our plate reader for.

Contacting Academics and Cancer researchers within the University to discuss about the microplate readers.

Research into potential buyers of our microplate reader from the biotechnology industry.

**engineering**
Adding a motorized system for moving between wells.

Testing the motor system and estimating the time taken to read a 48 well plate as roughly 3 mins.

**wiki and media design**
Main sections for the wiki decided.

Template design for the wiki made.

Our project name and logo made.

**human practices**
Market research for plate readers including specs, prices, competitors and consumer base.

Interview with an Academic, a cancer researcher, and a member of FabLab Barcelona(Community lab).

Skype call with iGEM Thessaly for possible collaboration.

[Read more](https://static.igem.org/mediawiki/2019/4/46/T--Sheffield--notebook_week3.pdf)

## Week 4

19 July 2019

**biology**
Preparing liquid cultures for four different bacterial strains.

**engineering**
Completing the first model of the plate reader with videos showing how the device moves in the x-axis and y-axis.

Receiving feedback on first model and inculcating the suggested improvements into the second model.

**wiki and media design**
 Decided on pastel colours for the background to have a minimalistic design.

**human practices**
Email exchange with a director of Media Lab Prado in Spain(Community lab) to discuss the benefit of having our device Open Source.

[Read more](https://static.igem.org/mediawiki/2019/f/fb/T--Sheffield--notebook_week4.pdf)

## Week 5

26 July 2019

**biology**
Contacting our competitors for specifications and prices of microplate readers they sell.

Preparing for the UK iGEM meetup in Newcastle by filling the biology section on the poster.

**engineering**
Finalising the improvements of the first model and building the second model in time for the UK iGEM meetup in Newcastle.

Filling the engineering section of the poster.

Researching into the principles of our presentation for the UK iGEM meetup

Practising the presentation for the UK iGEM meetup.

**wiki and media design**
Designing of poster for the UK iGEM meetup.

Designing of powerpoint slides for the UK iGEM meetup.

**human practices**
Deciding the awards to apply for in the Boston Jamboree.

Contacting biotechnology companies that could use our products.

[Read more](https://static.igem.org/mediawiki/2019/7/7d/T--Sheffield--notebook_week5.pdf)

## Week 6

02 August 2019

Congratulations for being awarded best presentation in the UK iGEM meetup.

**biology**
Receiving feedback from companies about specs, prices and comparing it with our device.

Testing of sensors in the plate reader with bacteria liquid cultures, and comparing with results from a standard spectrophotometer in the lab.

**engineering**
Designing a new model of our device based on feedback from the poster presentation and Q&A in the UK iGEM meetup e.g. 96 well plate, different sensors, modular filter system.

**wiki and media design**
Scheduling team photograph for the wiki.

Designing the animations to be incorporated in the wiki.

**human practices**
Collaborations with St Andrews iGEM, iGEM Nottingham, Manchester iGEM and iGEM Edinburgh proposed following our outing in Newcastle for the UK iGEM meetup.

[Read more](https://static.igem.org/mediawiki/2019/8/8e/T--Sheffield--notebook_week6.pdf)

## Week 7 - 13

09 august 2019

**biology**
Perform well plate reading experiments and statistically analyse results.

**engineering**
 Complete making a functional prototype.

Complete making a functional prototype, altering and fixing any problems faced with the machine.

Calibration experiments and analysis of growth curves.

**wiki and media design**
 Deciding which team member is writing each section of the wiki.

Writing-up Wiki content.

**human practices**
Interviewing biotechnology companies.

Completing collaboration activity with St Andrews iGEM by outreach events to high schools in Sheffield.

[Read more](https://static.igem.org/mediawiki/2019/1/1e/T--Sheffield--notebook_week7.pdf)

## Week 14-22

13 September 2019

**biology**
Prep samples sent by other iGEM teams to test with our microplate reader as part of collaboration.

Analyse results and provide feedback to other Igem teams that we are collaborating with.

**engineering**
Finalise the software and UI for the microplate reader .

Finalise and test run the IOT for the microplate reader.

**wiki and media design**
Finish writing up the wiki.

Prepare poster and practice presentation for the Igem Jamboree in Boston.

**human practices**
Complete collaborations with iGEM Manchester, iGEM Edinburgh, iGEM Nottingham, iGEM St Andrews, iGEM Madrid.

Presentation at the University of Sheffield departments of Bioengineering, Biomedical Sciences, Molecular Biology and Biotechnology.

[Read more](https://static.igem.org/mediawiki/2019/1/1d/T--Sheffield--notebook_week8.pdf)

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# Team:Sheffield/Experiments

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[HOME](https://2019.igem.org/Team%3ASheffield)

TEAM

[Team Members](https://2019.igem.org/Team%3ASheffield/Team)
[Collaborations](https://2019.igem.org/Team%3ASheffield/Collaborations)

PROJECT

[Description](https://2019.igem.org/Team%3ASheffield/Description)
[Design](https://2019.igem.org/Team%3ASheffield/Design)
[Experiments](https://2019.igem.org/Team%3ASheffield/Experiments)
[Notebook](https://2019.igem.org/Team%3ASheffield/Notebook)
[Contribution](https://2019.igem.org/Team%3ASheffield/Contribution)
[Results](https://2019.igem.org/Team%3ASheffield/Results)
[Demonstrate](https://2019.igem.org/Team%3ASheffield/Demonstrate)
[Improve](https://2019.igem.org/Team%3ASheffield/Improve)
[Attributions](https://2019.igem.org/Team%3ASheffield/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3ASheffield/Parts)
[Basic Parts](https://2019.igem.org/Team%3ASheffield/Basic_Part)
[Composite Parts](https://2019.igem.org/Team%3ASheffield/Composite_Part)
[Part Collection](https://2019.igem.org/Team%3ASheffield/Part_Collection)

[SAFETY](https://2019.igem.org/Team%3ASheffield/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3ASheffield/Human_Practices)
[Education & Engagement](https://2019.igem.org/Team%3ASheffield/Public_Engagement)

AWARDS

[Entrepreneurship](https://2019.igem.org/Team%3ASheffield/Entrepreneurship)
[Hardware](https://2019.igem.org/Team%3ASheffield/Hardware)
[Measurement](https://2019.igem.org/Team%3ASheffield/Measurement)
[Model](https://2019.igem.org/Team%3ASheffield/Model)
[Plant](https://2019.igem.org/Team%3ASheffield/Plant)
[Software](https://2019.igem.org/Team%3ASheffield/Software)

[JUDGING FORM ⇗](https://igem.org/2019_Judging_Form?team=Sheffield)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:Sheffield/Results

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[HOME](https://2019.igem.org/Team%3ASheffield)

TEAM

[Team Members](https://2019.igem.org/Team%3ASheffield/Team)
[Collaborations](https://2019.igem.org/Team%3ASheffield/Collaborations)

PROJECT

[Description](https://2019.igem.org/Team%3ASheffield/Description)
[Design](https://2019.igem.org/Team%3ASheffield/Design)
[Experiments](https://2019.igem.org/Team%3ASheffield/Experiments)
[Notebook](https://2019.igem.org/Team%3ASheffield/Notebook)
[Contribution](https://2019.igem.org/Team%3ASheffield/Contribution)
[Results](https://2019.igem.org/Team%3ASheffield/Results)
[Demonstrate](https://2019.igem.org/Team%3ASheffield/Demonstrate)
[Improve](https://2019.igem.org/Team%3ASheffield/Improve)
[Attributions](https://2019.igem.org/Team%3ASheffield/Attributions)

PARTS

[Parts Overview](https://2019.igem.org/Team%3ASheffield/Parts)
[Basic Parts](https://2019.igem.org/Team%3ASheffield/Basic_Part)
[Composite Parts](https://2019.igem.org/Team%3ASheffield/Composite_Part)
[Part Collection](https://2019.igem.org/Team%3ASheffield/Part_Collection)

[SAFETY](https://2019.igem.org/Team%3ASheffield/Safety)

HUMAN PRACTICES

[Human Practices](https://2019.igem.org/Team%3ASheffield/Human_Practices)
[Education & Engagement](https://2019.igem.org/Team%3ASheffield/Public_Engagement)

AWARDS

[Entrepreneurship](https://2019.igem.org/Team%3ASheffield/Entrepreneurship)
[Hardware](https://2019.igem.org/Team%3ASheffield/Hardware)
[Measurement](https://2019.igem.org/Team%3ASheffield/Measurement)
[Model](https://2019.igem.org/Team%3ASheffield/Model)
[Plant](https://2019.igem.org/Team%3ASheffield/Plant)
[Software](https://2019.igem.org/Team%3ASheffield/Software)

[JUDGING FORM ⇗](https://igem.org/2019_Judging_Form?team=Sheffield)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember **all measurement and characterization data must also be on the part's Main Page on the Registry.** Otherwise these data will not be in consideration for any medals or part awards!
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)