iGEM 2014 — Team British_Columbia

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# Team:British Columbia/Notebook

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| WELCOME TO iGEM 2014! Your team has been approved and you are ready to start the iGEM season!  On this page you can document your project, introduce your team members, document your progress   and share your iGEM experience with the rest of the world!   [Click here to edit this page!](https://2014.igem.org/wiki/index.php?title=Team:British_Columbia/Notebook&action=edit) | | |
=='''June 27'''==
===Transformation of parts BBa\_K801070, BBa\_K801071, BBa\_K801072 (2013 Kit, Plate 2, Wells 17H, 17J, 17L)===
'''Experimenters''': Grace Yi, Liz Geum

'''Aim''': To transform the TU Munich caffeine biosynthesis BioBricks from the Distribution Kit into *E. coli* chemically competent cells. This was also to test the cells' competency.

'''Results''': Eluted DNA from wells 17H, 17J, and 17L on plate 2 were transformed via heat-shock into the chemically competent cells made earlier. These were plated on LB agar/chloramphenicol plates and incubated overnight at 37 ºC. Transformation efficiency was found to be very low - two colonies per plate after more than 18 hours at 37 ºC.

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### Notebook

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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.

Hi, this is Alina testing!

| Date | Person | Notes |
| --- | --- | --- |
| 30th June 2014 | Jacky | Testing Wiki Page |

### JUNE 30, 2014

## TEST ENTRY

Experimenters: Albertina

FIRST ENTRY HERE

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