iGEM 2017 — Team ETH_Zurich

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:ETH Zurich/Notebook

[![CATE logo](https://static.igem.org/mediawiki/2017/d/d6/T--ETH_Zurich--LogoMinimal.png)](/Team%3AETH_Zurich)

* Project Idea
  + [Story of CATE](/Team%3AETH_Zurich/Description)
  + [CATE in Action](/Team%3AETH_Zurich/Applied_Design)
  + [Circuit](/Team%3AETH_Zurich/Circuit)
  + [Design](/Team%3AETH_Zurich/Design)
* Engineering

  + Dry Lab
  + [Overview](/Team%3AETH_Zurich/Model)
  + [Tumor Sensor](/Team%3AETH_Zurich/Model/Environment_Sensing)
  + [Heat Sensor](/Team%3AETH_Zurich/Model/Heat_Sensor)
  + [3D Model](/Team%3AETH_Zurich/Model/In_Vivo)
  + [Final Integration](/Team%3AETH_Zurich/Model/In_Silico_Final)
  + Wet Lab
  + [Experiments](/Team%3AETH_Zurich/Experiments)
  + [Lab Journal](/Team%3AETH_Zurich/Notebook)
  + [Protocols](/Team%3AETH_Zurich/Protocols)
  + [Safety](/Team%3AETH_Zurich/Safety)
  + [Sequences](/Team%3AETH_Zurich/Sequences)

  * Achievements
    + [Results](/Team%3AETH_Zurich/Results)
    + [Parts](/Team%3AETH_Zurich/Parts)
    + [Medal Criteria](/Team%3AETH_Zurich/Medal_Criteria)
    + [InterLab](/Team%3AETH_Zurich/InterLab)
    + [Collaborations](/Team%3AETH_Zurich/Collaborations)
  * Human Practices
    + [Overview](/Team%3AETH_Zurich/Human_Practices)
    + [Integrated Practices](/Team%3AETH_Zurich/HP/Gold_Integrated)
    + [Public Engagement](/Team%3AETH_Zurich/HP/Silver)* Our Team
      + [Attributions](/Team%3AETH_Zurich/Attributions)
      + [Members](/Team%3AETH_Zurich/Team)
      + [Sponsors](/Team%3AETH_Zurich/Sponsors)

# Notebook

Our team came together in April 2017. We first went through a period of extensive research and brainstorming, determined to find a novel solution to a real-world issue. Once we settled on our project in June, we focused on preparing a detailed plan of the work during summer. We designed our parts, circuits and experiments, distributed tasks, managed our team and our lab. Since July, we have been spending long hours in the lab, trying to get CATE ready for the Jamboree.

Continue reading to learn about our day-to-day activities in the lab.

July

August

September

October

Top

* ![Email](https://2017.igem.org/wiki/images/d/db/T--ETH_Zurich--EMail.svg "Email")
* [![Facebook](https://2017.igem.org/wiki/images/5/5c/T--ETH_Zurich--Facebook.svg "Facebook")](https://www.facebook.com/iGEM.ETH.Zurich)
* [![Twitter](https://2017.igem.org/wiki/images/8/8e/T--ETH_Zurich--Twitter.svg "Twitter")](https://twitter.com/eth_igem)

[![ETH Zurich](https://2017.igem.org/wiki/images/5/5d/T--ETH_Zurich--ETHZ_logo.png)](https://www.ethz.ch)
[Sponsors](/Team%3AETH_Zurich/Sponsors)

* [![Department of Biosystems Science and Engineering](https://2017.igem.org/wiki/images/e/e2/T--ETH_Zurich--DBSSE_logo.png)](https://www.bsse.ethz.ch/)
* [![Geneious](https://2017.igem.org/wiki/images/b/b0/T--ETH_Zurich--Geneious_logo.png)](https://www.geneious.com/)
* [![GenScript](https://2017.igem.org/wiki/images/6/67/T--ETH_Zurich--Genscript_logo.png)](https://www.genscript.com)
* [![Swiss Industry Science Fund](https://2017.igem.org/wiki/images/6/6c/T--ETH_Zurich--SISF_wide.png)](http://www.sisf.info/)
* [![University Children's hospital Zurich](https://2017.igem.org/wiki/images/9/93/T--ETH_Zurich--Childrens_Hospital_Zurich_logo.jpg)](https://www.kispi.uzh.ch)
* [![Microsynth AG](https://2017.igem.org/wiki/images/c/c9/T--ETH_Zurich--Microsynth_logo.png)](https://www.microsynth.ch/)
* [![Integrated DNA Technologies](https://static.igem.org/mediawiki/2017/8/80/IDT_logo.png)](https://www.idtdna.com)
* [![Amgen](https://2017.igem.org/wiki/images/6/61/T--ETH_Zurich--Amgen_logo.jpeg)](https://www.amgen.com)
* [![NCCR Molecular Systems Engineering](https://2017.igem.org/wiki/images/4/43/T--ETH_Zurich--NCCR_logo.jpg)](http://nccr-mse.ch)
* [![Lucerna Chem](https://2017.igem.org/wiki/images/1/17/T--ETH_Zurich--LucernaChem_logo.png)](https://lucerna-chem.ch)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:ETH Zurich/Engineering

[![CATE logo](https://static.igem.org/mediawiki/2017/d/d6/T--ETH_Zurich--LogoMinimal.png)](/Team%3AETH_Zurich)

* Project Idea
  + [Story of CATE](/Team%3AETH_Zurich/Description)
  + [CATE in Action](/Team%3AETH_Zurich/Applied_Design)
  + [Circuit](/Team%3AETH_Zurich/Circuit)
  + [Design](/Team%3AETH_Zurich/Design)
* Engineering

  + Dry Lab
  + [Overview](/Team%3AETH_Zurich/Model)
  + [Tumor Sensor](/Team%3AETH_Zurich/Model/Environment_Sensing)
  + [Heat Sensor](/Team%3AETH_Zurich/Model/Heat_Sensor)
  + [3D Model](/Team%3AETH_Zurich/Model/In_Vivo)
  + [Final Integration](/Team%3AETH_Zurich/Model/In_Silico_Final)
  + Wet Lab
  + [Experiments](/Team%3AETH_Zurich/Experiments)
  + [Lab Journal](/Team%3AETH_Zurich/Notebook)
  + [Protocols](/Team%3AETH_Zurich/Protocols)
  + [Safety](/Team%3AETH_Zurich/Safety)
  + [Sequences](/Team%3AETH_Zurich/Sequences)

  * Achievements
    + [Results](/Team%3AETH_Zurich/Results)
    + [Parts](/Team%3AETH_Zurich/Parts)
    + [Medal Criteria](/Team%3AETH_Zurich/Medal_Criteria)
    + [InterLab](/Team%3AETH_Zurich/InterLab)
    + [Collaborations](/Team%3AETH_Zurich/Collaborations)
  * Human Practices
    + [Overview](/Team%3AETH_Zurich/Human_Practices)
    + [Integrated Practices](/Team%3AETH_Zurich/HP/Gold_Integrated)
    + [Public Engagement](/Team%3AETH_Zurich/HP/Silver)* Our Team
      + [Attributions](/Team%3AETH_Zurich/Attributions)
      + [Members](/Team%3AETH_Zurich/Team)
      + [Sponsors](/Team%3AETH_Zurich/Sponsors)

# Engineering

I am the Engineering page and I am here to guide you!

* [Model](/Team%3AETH_Zurich/Model)
* [Protocols](/Team%3AETH_Zurich/Experiments)
* [Lab Journal](/Team%3AETH_Zurich/Notebook)
* [Results](/Team%3AETH_Zurich/Results)
* [Safety](/Team%3AETH_Zurich/Safety)
* [Collaborations](/Team%3AETH_Zurich/Collaborations)
* [InterLab](/Team%3AETH_Zurich/InterLab)

Top

* ![Email](https://2017.igem.org/wiki/images/d/db/T--ETH_Zurich--EMail.svg "Email")
* [![Facebook](https://2017.igem.org/wiki/images/5/5c/T--ETH_Zurich--Facebook.svg "Facebook")](https://www.facebook.com/iGEM.ETH.Zurich)
* [![Twitter](https://2017.igem.org/wiki/images/8/8e/T--ETH_Zurich--Twitter.svg "Twitter")](https://twitter.com/eth_igem)

[![ETH Zurich](https://2017.igem.org/wiki/images/5/5d/T--ETH_Zurich--ETHZ_logo.png)](https://www.ethz.ch)
[Sponsors](/Team%3AETH_Zurich/Sponsors)

* [![Department of Biosystems Science and Engineering](https://2017.igem.org/wiki/images/e/e2/T--ETH_Zurich--DBSSE_logo.png)](https://www.bsse.ethz.ch/)
* [![Geneious](https://2017.igem.org/wiki/images/b/b0/T--ETH_Zurich--Geneious_logo.png)](https://www.geneious.com/)
* [![GenScript](https://2017.igem.org/wiki/images/6/67/T--ETH_Zurich--Genscript_logo.png)](https://www.genscript.com)
* [![Swiss Industry Science Fund](https://2017.igem.org/wiki/images/6/6c/T--ETH_Zurich--SISF_wide.png)](http://www.sisf.info/)
* [![University Children's hospital Zurich](https://2017.igem.org/wiki/images/9/93/T--ETH_Zurich--Childrens_Hospital_Zurich_logo.jpg)](https://www.kispi.uzh.ch)
* [![Microsynth AG](https://2017.igem.org/wiki/images/c/c9/T--ETH_Zurich--Microsynth_logo.png)](https://www.microsynth.ch/)
* [![Integrated DNA Technologies](https://static.igem.org/mediawiki/2017/8/80/IDT_logo.png)](https://www.idtdna.com)
* [![Amgen](https://2017.igem.org/wiki/images/6/61/T--ETH_Zurich--Amgen_logo.jpeg)](https://www.amgen.com)
* [![NCCR Molecular Systems Engineering](https://2017.igem.org/wiki/images/4/43/T--ETH_Zurich--NCCR_logo.jpg)](http://nccr-mse.ch)
* [![Lucerna Chem](https://2017.igem.org/wiki/images/1/17/T--ETH_Zurich--LucernaChem_logo.png)](https://lucerna-chem.ch)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:ETH Zurich/Experiments

[![CATE logo](https://static.igem.org/mediawiki/2017/d/d6/T--ETH_Zurich--LogoMinimal.png)](/Team%3AETH_Zurich)

* Project Idea
  + [Story of CATE](/Team%3AETH_Zurich/Description)
  + [CATE in Action](/Team%3AETH_Zurich/Applied_Design)
  + [Circuit](/Team%3AETH_Zurich/Circuit)
  + [Design](/Team%3AETH_Zurich/Design)
* Engineering

  + Dry Lab
  + [Overview](/Team%3AETH_Zurich/Model)
  + [Tumor Sensor](/Team%3AETH_Zurich/Model/Environment_Sensing)
  + [Heat Sensor](/Team%3AETH_Zurich/Model/Heat_Sensor)
  + [3D Model](/Team%3AETH_Zurich/Model/In_Vivo)
  + [Final Integration](/Team%3AETH_Zurich/Model/In_Silico_Final)
  + Wet Lab
  + [Experiments](/Team%3AETH_Zurich/Experiments)
  + [Lab Journal](/Team%3AETH_Zurich/Notebook)
  + [Protocols](/Team%3AETH_Zurich/Protocols)
  + [Safety](/Team%3AETH_Zurich/Safety)
  + [Sequences](/Team%3AETH_Zurich/Sequences)

  * Achievements
    + [Results](/Team%3AETH_Zurich/Results)
    + [Parts](/Team%3AETH_Zurich/Parts)
    + [Medal Criteria](/Team%3AETH_Zurich/Medal_Criteria)
    + [InterLab](/Team%3AETH_Zurich/InterLab)
    + [Collaborations](/Team%3AETH_Zurich/Collaborations)
  * Human Practices
    + [Overview](/Team%3AETH_Zurich/Human_Practices)
    + [Integrated Practices](/Team%3AETH_Zurich/HP/Gold_Integrated)
    + [Public Engagement](/Team%3AETH_Zurich/HP/Silver)* Our Team
      + [Attributions](/Team%3AETH_Zurich/Attributions)
      + [Members](/Team%3AETH_Zurich/Team)
      + [Sponsors](/Team%3AETH_Zurich/Sponsors)

# Experiments

*From this page you can access detailed descriptions of the experiments we've done. To skip the details and quickly find out about most important achievements, check out [Results.](https://2017.igem.org/Team%3AETH_Zurich/Results) To read about how we structured and planned our work, see the [Design.](https://2017.igem.org/Team%3AETH_Zurich/Design)*

# Overview

We divided our experiments into five categories corresponding to CATE's five functions (see [Circuit](https://2017.igem.org/Team%3AETH_Zurich/Circuit)). We worked in parallel on individual functions and combined them together once they reached the desired behaviour.

Our work was planned in three phases (see [Design](https://2017.igem.org/Team%3AETH_Zurich/Design)). In *Phase I: Initial Design* we choose and optimized DNA sequences for our parts based on literature research and planned assembly of the individual parts into test devices. The test devices were then used to develop working assays. In *Phase II: Tests and Optimization*, the assays thought us about how our functions behave. Based on this information, we could tune different parameters to optimize subsequent experiments. In *Phase III: Demonstration of the Function*, milestone experiments were performed with the optimized parameters to show our systems in action.

Access detailed descriptions of all our experiments here:

* [Tumor Sensor](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Tumor_Sensor)
* [MRI Contrast Agent](https://2017.igem.org/Team%3AETH_Zurich/Experiments/MRI_Contrast_Agent)
* [Anti-Cancer Toxin](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Anti_Cancer_Toxin)
* [Heat Sensor](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Heat_Sensor)
* [Cell Lysis](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Cell_Lysis)

Top

* ![Email](https://2017.igem.org/wiki/images/d/db/T--ETH_Zurich--EMail.svg "Email")
* [![Facebook](https://2017.igem.org/wiki/images/5/5c/T--ETH_Zurich--Facebook.svg "Facebook")](https://www.facebook.com/iGEM.ETH.Zurich)
* [![Twitter](https://2017.igem.org/wiki/images/8/8e/T--ETH_Zurich--Twitter.svg "Twitter")](https://twitter.com/eth_igem)

[![ETH Zurich](https://2017.igem.org/wiki/images/5/5d/T--ETH_Zurich--ETHZ_logo.png)](https://www.ethz.ch)
[Sponsors](/Team%3AETH_Zurich/Sponsors)

* [![Department of Biosystems Science and Engineering](https://2017.igem.org/wiki/images/e/e2/T--ETH_Zurich--DBSSE_logo.png)](https://www.bsse.ethz.ch/)
* [![Geneious](https://2017.igem.org/wiki/images/b/b0/T--ETH_Zurich--Geneious_logo.png)](https://www.geneious.com/)
* [![GenScript](https://2017.igem.org/wiki/images/6/67/T--ETH_Zurich--Genscript_logo.png)](https://www.genscript.com)
* [![Swiss Industry Science Fund](https://2017.igem.org/wiki/images/6/6c/T--ETH_Zurich--SISF_wide.png)](http://www.sisf.info/)
* [![University Children's hospital Zurich](https://2017.igem.org/wiki/images/9/93/T--ETH_Zurich--Childrens_Hospital_Zurich_logo.jpg)](https://www.kispi.uzh.ch)
* [![Microsynth AG](https://2017.igem.org/wiki/images/c/c9/T--ETH_Zurich--Microsynth_logo.png)](https://www.microsynth.ch/)
* [![Integrated DNA Technologies](https://static.igem.org/mediawiki/2017/8/80/IDT_logo.png)](https://www.idtdna.com)
* [![Amgen](https://2017.igem.org/wiki/images/6/61/T--ETH_Zurich--Amgen_logo.jpeg)](https://www.amgen.com)
* [![NCCR Molecular Systems Engineering](https://2017.igem.org/wiki/images/4/43/T--ETH_Zurich--NCCR_logo.jpg)](http://nccr-mse.ch)
* [![Lucerna Chem](https://2017.igem.org/wiki/images/1/17/T--ETH_Zurich--LucernaChem_logo.png)](https://lucerna-chem.ch)

[![](https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg)](https://2017.igem.org)

[![](https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg)](https://2017.igem.org)

# Team:ETH Zurich/Results

[![CATE logo](https://static.igem.org/mediawiki/2017/d/d6/T--ETH_Zurich--LogoMinimal.png)](/Team%3AETH_Zurich)

* Project Idea
  + [Story of CATE](/Team%3AETH_Zurich/Description)
  + [CATE in Action](/Team%3AETH_Zurich/Applied_Design)
  + [Circuit](/Team%3AETH_Zurich/Circuit)
  + [Design](/Team%3AETH_Zurich/Design)
* Engineering

  + Dry Lab
  + [Overview](/Team%3AETH_Zurich/Model)
  + [Tumor Sensor](/Team%3AETH_Zurich/Model/Environment_Sensing)
  + [Heat Sensor](/Team%3AETH_Zurich/Model/Heat_Sensor)
  + [3D Model](/Team%3AETH_Zurich/Model/In_Vivo)
  + [Final Integration](/Team%3AETH_Zurich/Model/In_Silico_Final)
  + Wet Lab
  + [Experiments](/Team%3AETH_Zurich/Experiments)
  + [Lab Journal](/Team%3AETH_Zurich/Notebook)
  + [Protocols](/Team%3AETH_Zurich/Protocols)
  + [Safety](/Team%3AETH_Zurich/Safety)
  + [Sequences](/Team%3AETH_Zurich/Sequences)

  * Achievements
    + [Results](/Team%3AETH_Zurich/Results)
    + [Parts](/Team%3AETH_Zurich/Parts)
    + [Medal Criteria](/Team%3AETH_Zurich/Medal_Criteria)
    + [InterLab](/Team%3AETH_Zurich/InterLab)
    + [Collaborations](/Team%3AETH_Zurich/Collaborations)
  * Human Practices
    + [Overview](/Team%3AETH_Zurich/Human_Practices)
    + [Integrated Practices](/Team%3AETH_Zurich/HP/Gold_Integrated)
    + [Public Engagement](/Team%3AETH_Zurich/HP/Silver)* Our Team
      + [Attributions](/Team%3AETH_Zurich/Attributions)
      + [Members](/Team%3AETH_Zurich/Team)
      + [Sponsors](/Team%3AETH_Zurich/Sponsors)

# Results

![ETHZ 2017 at Giant Jamboree](https://static.igem.org/mediawiki/2017/0/0d/T--ETH_Zurich--GiantJamboree.jpg)

# Achievements

* Best Model, Overgrad
* Best Poster, Overgrad
* Second place for Best Therapeutics, Overgrad
* Nominated for Best New Basic Part, Overgrad

# Tumor Sensor

* [Designed parts rationally](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Tumor_Sensor#phaseI), according to a  [preliminary functioning point search](https://2017.igem.org/Team%3AETH_Zurich/Model/Environment_Sensing/parameter_space) thanks to our model.
* [Designed and realized](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Tumor_Sensor#overview) the most relevant experiments to [precisely characterize](https://2017.igem.org/Team%3AETH_Zurich/Model/Environment_Sensing/parameter_fitting) our quorum-sensing system.
* [Designed a hybrid promoter](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Tumor_Sensor#phaseI) that implements AND-gate logic evaluation of L-lactate and AHL and successfully demonstrated its operation.
* [Characterized several versions](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Tumor_Sensor#AND_gate_wo_QS) of this hybrid promoter and [improved the model](https://2017.igem.org/wiki/index.php?title=Team:ETH_Zurich/Model/Environment_Sensing/AND_gate_fitting#improve) of the hybrid promoter from the observed experimental behavior.
* [Step](https://2017.igem.org/Team%3AETH_Zurich/Model/Environment_Sensing/parameter_fitting) by [step](https://2017.igem.org/wiki/index.php?title=Team:ETH_Zurich/Model/Environment_Sensing/AND_gate_fitting#fitand) fitting of the most relevant parameters controlling the lactate and bacterial cell density sensing.
* Using our model, we could [choose the best version](https://2017.igem.org/Team%3AETH_Zurich/Model/In_Silico_Final) of AND-gate such that it is capable of distinguishing lactate levels and bacterial population densities associated with healthy and tumor tissue.

[Description](https://2017.igem.org/Team%3AETH_Zurich/Circuit/Fa_Tumor_Sensor)

[Experiments](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Tumor_Sensor)

[Model](https://2017.igem.org/Team%3AETH_Zurich/Model/Environment_Sensing)

# MRI Contrast Agent

* [Characterized the expression](https://2017.igem.org/Team%3AETH_Zurich/Experiments/MRI_Contrast_Agent#phaseII) of a genetically encoded MRI contrast agent bacterioferritin in E. coli Nissle 1917.
* Used our data to [fit parameters](https://2017.igem.org/Team%3AETH_Zurich/Model/Environment_Sensing/parameter_fitting#luxr_fit) of the model.
* Showed that the contrast agent indeed leads to a [marked decrease in the MRI signal](https://2017.igem.org/Team%3AETH_Zurich/Experiments/MRI_Contrast_Agent#phaseIII) which demonstrates its usability as an MRI contrast agent in vitro and confirms the potential to use it as an in vivo reporter of tumor sensing.

[Description](https://2017.igem.org/Team%3AETH_Zurich/Circuit/Fb_MRI_Contrast_Agent)

[Experiments](https://2017.igem.org/Team%3AETH_Zurich/Experiments/MRI_Contrast_Agent)

[Model](https://2017.igem.org/Team%3AETH_Zurich/Model/Environment_Sensing/parameter_fitting#luxr_fit)

# Anti-Cancer Toxin

* [Developed an assay](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Anti_Cancer_Toxin#phaseII) to characterize killing of mammalian cells when supplemented with supernatant of lysed bacteria expressing azurin.
* Expressed it successfully in our bacteria.
* Produced azurin in a controlable manner (via AHL induction).
* Produced an azurin-rich bacterial lysate.
* [Absolute quantification](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Anti_Cancer_Toxin#phaseIII) of azurin in our lysate.
* [Quantitative assessment](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Anti_Cancer_Toxin#phaseIII) of cytotoxic effect of azurin on p53 positive and negative cancer cell lines.
* Quantified the potential  [treatment efficiency](https://2017.igem.org/Team%3AETH_Zurich/Model/In_Vivo#InVivo_Killing) of our system.

[Description](https://2017.igem.org/Team%3AETH_Zurich/Circuit/Fc_Anti_Cancer_Toxin)

[Experiments](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Anti_Cancer_Toxin)

[Model](https://2017.igem.org/wiki/index.php?title=Team:ETH_Zurich/Model/In_Vivo#InVivo_Killing)

# Heat Sensor

* [Built and characterized](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Heat_Sensor#phaseII) a thermoresponsive system that induces expression of a controlled gene at 45 °C but not at 37 °C.
* Validate with a [thermal diffusion model](https://2017.igem.org/Team%3AETH_Zurich/Model/Heat_Sensor) the clinical feasibility of a 45 °C induction in the context of our application.
* Built an [RBS library](https://2017.igem.org/Team%3AETH_Zurich/Model/Heat_Sensor#phaseII) for the Heat Sensor, whereby we reduced leakiness far enough to transform the potent lysis-inducing protein E under its control.

[Description](https://2017.igem.org/Team%3AETH_Zurich/Circuit/Fd_Heat_Sensor)

[Experiments](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Heat_Sensor)

[Model](https://2017.igem.org/wiki/index.php?title=Team:ETH_Zurich/Model/Heat_Sensor)

# Cell Lysis

* Induced [gfp release to supernatant](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Cell_Lysis#phaseIII) through temperature (45 °C) induced cell lysis.

[Description](https://2017.igem.org/Team%3AETH_Zurich/Circuit/Fe_Cell_Lysis)

[Experiments](https://2017.igem.org/Team%3AETH_Zurich/Experiments/Cell_Lysis)

# Human Practices

* Brought synthetic biology and bacterial cancer therapy closer to a broad range of people.
* Learned from various experts to think about aspects of our project that go beyond the lab.

[Overview](https://2017.igem.org/Team%3AETH_Zurich/Human_Practices)

[Integrated Practices](https://2017.igem.org/Team%3AETH_Zurich/HP/Gold_Integrated)

[Public Engagement](https://2017.igem.org/Team%3AETH_Zurich/HP/Silver)

# Poster

![ETHZ 2017 Giant Jamboree Poster](https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PosterLQ.png)

[Download Poster PDF (85 MB)](https://static.igem.org/mediawiki/2017/0/06/T--ETH_Zurich--Colorful_CATE_Poster.pdf)

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* [![Department of Biosystems Science and Engineering](https://2017.igem.org/wiki/images/e/e2/T--ETH_Zurich--DBSSE_logo.png)](https://www.bsse.ethz.ch/)
* [![Geneious](https://2017.igem.org/wiki/images/b/b0/T--ETH_Zurich--Geneious_logo.png)](https://www.geneious.com/)
* [![GenScript](https://2017.igem.org/wiki/images/6/67/T--ETH_Zurich--Genscript_logo.png)](https://www.genscript.com)
* [![Swiss Industry Science Fund](https://2017.igem.org/wiki/images/6/6c/T--ETH_Zurich--SISF_wide.png)](http://www.sisf.info/)
* [![University Children's hospital Zurich](https://2017.igem.org/wiki/images/9/93/T--ETH_Zurich--Childrens_Hospital_Zurich_logo.jpg)](https://www.kispi.uzh.ch)
* [![Microsynth AG](https://2017.igem.org/wiki/images/c/c9/T--ETH_Zurich--Microsynth_logo.png)](https://www.microsynth.ch/)
* [![Integrated DNA Technologies](https://static.igem.org/mediawiki/2017/8/80/IDT_logo.png)](https://www.idtdna.com)
* [![Amgen](https://2017.igem.org/wiki/images/6/61/T--ETH_Zurich--Amgen_logo.jpeg)](https://www.amgen.com)
* [![NCCR Molecular Systems Engineering](https://2017.igem.org/wiki/images/4/43/T--ETH_Zurich--NCCR_logo.jpg)](http://nccr-mse.ch)
* [![Lucerna Chem](https://2017.igem.org/wiki/images/1/17/T--ETH_Zurich--LucernaChem_logo.png)](https://lucerna-chem.ch)

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