iGEM 2017 — Team BNDS_China

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# Team:BNDS China/Notebook

[![](https://static.igem.org/mediawiki/2017/d/de/BNDS_China-Logob1.png)](https://2017.igem.org/Team%3ABNDS_China)

Team

* [Team](https://2017.igem.org/Team%3ABNDS_China/Team)
* [Collaborations](https://2017.igem.org/Team%3ABNDS_China/Collaborations)

Project

* [Description](https://2017.igem.org/Team%3ABNDS_China/Description)
* [Design](https://2017.igem.org/Team%3ABNDS_China/Design)
* [Experiment](https://2017.igem.org/Team%3ABNDS_China/Experiments)
* [InterLab](https://2017.igem.org/Team%3ABNDS_China/InterLab)
* [Demonstrate](https://2017.igem.org/Team%3ABNDS_China/Demonstrate)
* [Attributions](https://2017.igem.org/Team%3ABNDS_China/Attributions)

[Parts](https://2017.igem.org/Team%3ABNDS_China/Parts)

[Safety](https://2017.igem.org/Team%3ABNDS_China/Safety)

H.Prac

* [Silver HP](https://2017.igem.org/Team%3ABNDS_China/HP/Silver)
* [Integrated and Gold](https://2017.igem.org/Team%3ABNDS_China/HP/Gold_Integrated)
* [Public Engagement](https://2017.igem.org/Team%3ABNDS_China/Engagement)

[Awards](https://2017.igem.org/Team%3ABNDS_China/Model)

[Judge](https://igem.org/2017_Judging_Form?team=BNDS_China)

![](https://static.igem.org/mediawiki/2017/e/ee/BNDS_China-Top.png)

### Notebook

##### Q5 PCR Reaction system (50 μL) for the vector RPG

1. Q5 Reaction Buffer (5x): 10 μL
2. dNTP: 3 μL
3. Forward Primer/ Reverse Primer :2.5 μL/2.5 μL
4. Template (RPG): 45ng
5. Q5 DNA Polymerase: 0.5 μL
6. ddH2O：31 μL

---

##### Transformation system:

Positive control: 25 μL competent cell + 1 μL RPG

Negative control: 25 μL competent cell

Experiment group (with Gibson products): 50 μL competent cells + 2 μL Gibson products

1. Pick 6-10 colonies.
2. Add 20 μL Amp+ liquid LB broth into 0.2 mL microfuge tube and add colonies, respectively. Then use 37°C shaker (with rotate speed 270r/min) to revive the bacteria for 1 hour.

---

##### Colony PCR Reaction System:

1. Taq Mix: 12.5 μL
2. Forward Primer/ Reverse Primer: 1 μL/1 μL
3. Bacteria LB solution: 2 μL
4. ddH2O: 10.5 μL

© 2017 BNDS\_China All Rights Reserved.

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# Team:BNDS China/Experiments

[![](https://static.igem.org/mediawiki/2017/d/de/BNDS_China-Logob1.png)](https://2017.igem.org/Team%3ABNDS_China)

Team

* [Team](https://2017.igem.org/Team%3ABNDS_China/Team)
* [Collaborations](https://2017.igem.org/Team%3ABNDS_China/Collaborations)

Project

* [Description](https://2017.igem.org/Team%3ABNDS_China/Description)
* [Design](https://2017.igem.org/Team%3ABNDS_China/Design)
* [Experiment](https://2017.igem.org/Team%3ABNDS_China/Experiments)
* [InterLab](https://2017.igem.org/Team%3ABNDS_China/InterLab)
* [Demonstrate](https://2017.igem.org/Team%3ABNDS_China/Demonstrate)
* [Attributions](https://2017.igem.org/Team%3ABNDS_China/Attributions)

[Parts](https://2017.igem.org/Team%3ABNDS_China/Parts)

[Safety](https://2017.igem.org/Team%3ABNDS_China/Safety)

H.Prac

* [Silver HP](https://2017.igem.org/Team%3ABNDS_China/HP/Silver)
* [Integrated and Gold](https://2017.igem.org/Team%3ABNDS_China/HP/Gold_Integrated)
* [Public Engagement](https://2017.igem.org/Team%3ABNDS_China/Engagement)

[Awards](https://2017.igem.org/Team%3ABNDS_China/Model)

[Judge](https://igem.org/2017_Judging_Form?team=BNDS_China)

![](https://static.igem.org/mediawiki/2017/e/ee/BNDS_China-Top.png)

### Experiments

![](https://static.igem.org/mediawiki/2017/a/a5/20171028224559.png)

© 2017 BNDS\_China All Rights Reserved.

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# Team:BNDS China/Results

[![](https://static.igem.org/mediawiki/2017/d/de/BNDS_China-Logob1.png)](https://2017.igem.org/Team%3ABNDS_China)

Team

* [Team](https://2017.igem.org/Team%3ABNDS_China/Team)
* [Collaborations](https://2017.igem.org/Team%3ABNDS_China/Collaborations)

Project

* [Description](https://2017.igem.org/Team%3ABNDS_China/Description)
* [Design](https://2017.igem.org/Team%3ABNDS_China/Design)
* [Experiment](https://2017.igem.org/Team%3ABNDS_China/Experiments)
* [InterLab](https://2017.igem.org/Team%3ABNDS_China/InterLab)
* [Demonstrate](https://2017.igem.org/Team%3ABNDS_China/Demonstrate)
* [Attributions](https://2017.igem.org/Team%3ABNDS_China/Attributions)

[Parts](https://2017.igem.org/Team%3ABNDS_China/Parts)

[Safety](https://2017.igem.org/Team%3ABNDS_China/Safety)

H.Prac

* [Silver HP](https://2017.igem.org/Team%3ABNDS_China/HP/Silver)
* [Integrated and Gold](https://2017.igem.org/Team%3ABNDS_China/HP/Gold_Integrated)
* [Public Engagement](https://2017.igem.org/Team%3ABNDS_China/Engagement)

[Awards](https://2017.igem.org/Team%3ABNDS_China/Model)

[Judge](https://igem.org/2017_Judging_Form?team=BNDS_China)

![](https://static.igem.org/mediawiki/2017/e/ee/BNDS_China-Top.png)

# Results

Here you can describe the results of your project and your future plans.

##### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

##### You should also describe what your results mean:

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

##### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

##### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)

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