iGEM 2019 — Team HZAU-China

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:HZAU-China/Notebook

* Project
  + [Description](https://2019.igem.org/Team%3AHZAU-China/Description)
  + [Design](https://2019.igem.org/Team%3AHZAU-China/Design)
  + [Demonstrate](https://2019.igem.org/Team%3AHZAU-China/Demonstrate)
  + [Notebook](https://2019.igem.org/Team%3AHZAU-China/Notebook)
* Wetlab
  + [Lab Notes](https://2019.igem.org/Team%3AHZAU-China/Lab_Notes)
  + [Protocol](https://2019.igem.org/Team%3AHZAU-China/Protocol)
  + [Safety](https://2019.igem.org/Team%3AHZAU-China/Safety)
  + [Parts](https://2019.igem.org/Team%3AHZAU-China/Parts)
  + [Composite\_Part](https://2019.igem.org/Team%3AHZAU-China/Composite_Part)
* Drylab
  + [Model](https://2019.igem.org/Team%3AHZAU-China/Model)
  + [Hardware](https://2019.igem.org/Team%3AHZAU-China/Hardware)
  + [Software](https://2019.igem.org/Team%3AHZAU-China/Software)
* Human Practices
  + [Collaborations](https://2019.igem.org/Team%3AHZAU-China/Collaborations)
  + [Human\_Practices](https://2019.igem.org/Team%3AHZAU-China/Human_Practices)
  + [Public\_Engagement](https://2019.igem.org/Team%3AHZAU-China/Public_Engagement)
* About us
  + [Team](https://2019.igem.org/Team%3AHZAU-China/Team)
  + [Attributions](https://2019.igem.org/Team%3AHZAU-China/Attributions)

[![](https://static.igem.org/mediawiki/2019/5/52/T--HZAU-China--logo3.png)](https://2019.igem.org/Team%3AHZAU-China)

[![backtotop](https://static.igem.org/mediawiki/2019/d/da/T--HZAU-China--back_to_top.png
)](#back_to_top)

# December 2018

1. . Recruit new team members.
2. . Send assignments to new candidate members.
3. . Entrance Exam for new candidate members.

# January 2019

1. . Winter holiday training for new candidate members. The winter holiday training including presentations of past iGEM projects and journal share.
2. . Winter holiday assignments for new candidate members.

# February 2019

1. . Journal reading and concept design for new project.

# March 2019

1. . Winter holiday assignments checking.
2. . New members filtering. New members were selected basing on their performance on their assignments, winter holiday presentations and Entrance Exam.
3. . Brainstorm for new projects.
4. . New member training (experiments and modellings).

# April 2019

1. . Brainstorm for new projects.
2. . New member training (experiments and modellings).

# May 2019

1. . Search for theoretical background and design for the new projects.
2. . Recheck new projects. Notice: These projects were abandoned due to its similarity to other former projects.
3. . New members training (experiments and modellings).

# June 2019

1. . Little Future Scientists activity.
2. . New project confirmed, which was inspired by Little Future Scientists activity.
3. . New project theoretical design and rechecking.
4. . New members training (experiments and modellings).
5. . Make experiments and modellings arrangement.
6. . Collaboration with XHD-WS-Wuhan-B. We helped them with their project design.

# July 2019

1. . Part Characterization.
2. . Collaboration with team XHD-WS-Wuhan-B. We taught one of their students how to do molecular cloning.
3. . Construction of smell-sensing module. We planned to construct two kinds of smell-sensing module. One could sense geraniol and the other one could sense benzyl alcohol. But at last we chose the benzyl alcohol one as smell sensing module.
4. . Construction of quorum-sensing circuit.
5. . Construction of the switch of memory module. Preliminary verification of taRNA-crRNA interaction.
6. . Construction of reproduction module.
7. . Construction of the erasure module.We planned to construct two kinds of reproduction module. One could synthesize geraniol and the other one could synthesize benzyl alcohol. But in the end, we decided to take the benzyl alcohol one as the reproduction module.
8. . Construction of Genome-Scale Metabolic Model.
9. . Construction of models following
   the four modules.
10. . Starting to run our public social account.
11. . Online courses to pupils in mountainous area.

# August 2019

1. . Parts characterization revision.
2. . Parts improvement.
3. . Continue the experiments initiated in July.
4. . Construction of the reproduction module complete.
5. . Continue constructing the models of the four modules.
6. . Attend Conference of China iGEMer Community hold in Shenzhen, China.
7. . Attend Central China Meetup.
8. . Continue running our public social account.

# September 2019

1. . Parts improvement revision.
2. . Continue part characterization.
3. . The construction and verification of Smell sensing module complete.
4. . Revise the reproduction module.
5. . Continue constructing the reproduction module, the memory module and the erasure module.
6. . Collaboration with HBUT-China.We helped them build a model to evaluate the influence of nickel ion on yeast.
7. . Summarize and revise the models built in summer vacation.
8. . Propaganda of iGEM to new-entering students in Huazhong Agricultural University.

# October 2019

1. . Parts improvement complete.
2. . Part characterization complete.
3. . The reproduction module revision.
4. . Construction and verification of the memory module.
5. . Construction and verification of the erasure module.
6. . Verification of the revised reproduction module.
7. . Summarized the revised models.
8. . Collaboration with XHD-WS-Wuhan-A. We helped them with their part characterization.
9. . Wiki writing.
10. . Questionnaire about our project to society to find out what people expected for from our project.
11. . Investigating children with autism to find out how our project impact on them and what they expected for from our project.

![](https://static.igem.org/mediawiki/2019/8/86/T--HZAU-China--footer.png)


[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:HZAU-China/Experiments

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://2019.igem.org/wiki/images/8/8b/HQ_page_logo.jpg)](https://2019.igem.org)

# Team:HZAU-China/Results