iGEM 2018 — Team Rheda_Bielefeld

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# Team:Rheda Bielefeld/Notebook

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Team

[The Team](https://2018.igem.org/Team%3ARheda_Bielefeld/Team)
[Our School](https://2018.igem.org/Team%3ARheda_Bielefeld/School)
[Bielefeld University](https://2018.igem.org/Team%3ARheda_Bielefeld/University)
[Rheda](https://2018.igem.org/Team%3ARheda_Bielefeld/Rheda)

Project

[Description](https://2018.igem.org/Team%3ARheda_Bielefeld/Description)
[Notebook](https://2018.igem.org/Team%3ARheda_Bielefeld/Notebook)

[InterLab Study](https://2018.igem.org/Team%3ARheda_Bielefeld/InterLab)

Experiments

[Overview](https://2018.igem.org/Team%3ARheda_Bielefeld/OverviewExperiment)
[Methods](https://2018.igem.org/Team%3ARheda_Bielefeld/Methods)
[Pollen](https://2018.igem.org/Team%3ARheda_Bielefeld/Pollen)
[PCR](https://2018.igem.org/Team%3ARheda_Bielefeld/PCR)
[Assays](https://2018.igem.org/Team%3ARheda_Bielefeld/Assays)
[Cloning Strategy](https://2018.igem.org/Team%3ARheda_Bielefeld/Cloning_Strategy)

[Parts](https://2018.igem.org/Team%3ARheda_Bielefeld/Parts)

Practice

[Human Practices](https://2018.igem.org/Team%3ARheda_Bielefeld/Human_Practices)
[Marta Event](https://2018.igem.org/Team%3ARheda_Bielefeld/Marta_Event)

Safety

[Safety Form](https://2018.igem.org/Team%3ARheda_Bielefeld/Safety)
[Biosafety](https://2018.igem.org/Team%3ARheda_Bielefeld/Biosafety)

[Collaborations](https://2018.igem.org/Team%3ARheda_Bielefeld/Collaborations)

Attributions

[Attributions](https://2018.igem.org/Team%3ARheda_Bielefeld/Attributions)
[Partners](https://2018.igem.org/Team%3ARheda_Bielefeld/Partners)

[Judging Form ⇗](https://igem.org/2018_Judging_Form?team=Rheda_Bielefeld)

![](https://static.igem.org/mediawiki/2018/8/8b/T--Rheda_Bielefeld--Header%28notebook%2Celisa%29steribank.jpeg)

![](https://static.igem.org/mediawiki/2018/thumb/7/74/T--Rheda_Bielefeld--Notebook.JPG/450px-T--Rheda_Bielefeld--Notebook.JPG)
![](https://static.igem.org/mediawiki/2018/0/06/T--Rheda_Bielefeld--LeonSteri.jpg) Leon working at the flow cabinet
![](https://static.igem.org/mediawiki/2018/4/42/T--Rheda_Bielefeld--BenSchüttler.jpg)Ben putting colonies into the incubator

## Notebook

Wet-Lab Protocol

22.05.2018
Opening pollen with Trypsin
-Resuspending 20µg Trypsin in 200 µl of water
-Splitting solution in 4 eppis with 50 µl each => approxamittly 5 µg of Trypsin in each eppi
-5000 U/mg=Proportionalitycalculation
-50mg of pollenmaterial
-3 active Trypsin-eppis (incubating at 30 °C for 15 minutes)
-Approximately 15 µg of Trypsin available
Calculating units:
-1 mg= 5000 U
-1 solution: 15 µg= 0.015 mg= 75 U=> 1:3.3
-2 solutions: 7.5 µg=0.0075 mg= 37 U=> 1:6.6
-3 solutions: 5 µg=0.005 mg= 25 U => 1:10
-Proportionality=weight Trypsin : weight pollen
-Add 50 µg of pollen to 10µl Trypsin and 120µl Ammoniumhydrocarbonate
-Incubating at 37°C overnight
=> analyzed with light microscopy on 23.05.: did not work

-Microscopy Birchpollen (100x)
-Microscopy Birchpollen (400x)
-Microscopy Willowpollen (100x)
-Microscopy Willowpollen (400x)
-Microscopy Sprucepollen (100x)
-Microscopy Sprucepollen (400x)

Extraction of pollen
-Putting male infructescence into an electrostatically loaded plastic tube
-Vortexing tube => pollen stick to walls of the tube
-Extracting pollen
-Analyzing with light microscopy if only one type of pollen is in each tube

23.05.2018

Electromicroscopy

-Prepare samples
-> 1: birchpollen
-> 2: Spruce-trypsin supernatant
-> 3: Spruce-trypsin sediment
-> 4: SPruce and spruce ribolysed

-Dried in etikator
-Evaporated with gold
-DNA-extraction pollen
-50 mg in rybolyser tubes
-Constant shaking in rybolyser (6500\*2\*30\*30)
-> centrifugate for 10 min (1100 rpm)

Results:
-Two layers:
-> over the upper one: white shroud
-> light-brown layer of pollen: abstraction with chemical droppper
-> 400µl lysebuffer, centrifugate 1 min (11000 rpm)
-> DNA-extraction: machery nagel plant NUcleo spin III Kit

Nanodrop
Samples from leaves

| B | ng/µl   260/280   260/230 | 22,7   1,68   1,23 | 97,2   1,71   0,83 |
| --- | --- | --- | --- |
| PA | ng/µl   260/280   260/230 | 3,1  0,97   1,05 | 8,9   1,83   0,83 |
| PB | ng/µl  260/280   260/230 | 30,2   2,34   0,73 | 35,1   1,84   0,89 |

Opening pollen with liquid nitrogen
-Nitrogen and mortar and pestle
-Check: light microscope
Opening pollen with ribolyser

-10,20 & 50 mg pollen and 4oo µl water each in ribolyser tube
-Ribolyser (6500-3\*45-30)
->Just 10 mg worked (light microscope)

24.05.2018

Pollensamples evaporate with gold (10-20 nm)

| nitrogen | ng>/µl   260/280   260/230 | 66,6   1,88   1,69 | 75,6  1,71   1,33 |
| --- | --- | --- | --- |
| trypsin | ng/µl   260/280   260/230 | 418,2   1,72  1,00 | 399,2   1,59   0,86 |

DNA-extraction of pollen
-1: opened with trypsin
-2: opened with liquid nitrogen
-> 1: 400 µl PL1 and 1 pollen and 10µl RNA-se ( centrifugate 1min 11000 rpm)
-> 2: 800 µl PL1 and 2 pollen and 10 µl RNA-se ( centrifugate 2min 11000 rpm)
-> protocol DNA extraction

DNA-extraction from birchleafes
-Protocol
DNA-extraction from spruce needles
-Liquid nitrogen

17.07.2018

Extracted DNA: Nanodrop und PCR
-> 5x Phusion HF buffer: 36 µl
-> 10 mn dNTPs: 3.6 µl
-> Fw primer: 9µl
-> rw primer: 9µl
-> template DNA: 9µl
-> Phusion DNA polymerase: 1.8 µl
-> H2o: 111.6 µl
5 primer mixtures: - SP1
- sp2 V
- ec1 --> each with DNA sample and positive and negative control
-ec2
-bet

Results Nanodrop

| birch pollen+ nitrogen | 8ng/µl |
| --- | --- |
| spruce pollen+ nitrogen | 10,5 ng/µl |
| birch leaves+ mortar | 11,5 ng/µl |
| birch leaves+mortar | 5,5 ng/µl |
| birch leaves+ mortar | 17,5 ng/µl |

B. sub
-600µl 5c buffer+ cells from plate
-600 µl in ribolyser tubes
-Ribolyse
-Centrifugate: 5 min, 8000 rpm
-Take supernatant
+1ml NaCl
-Filtrate

Nanodrop B. Sub.

| Leon | ng/µl   260/280   260/230 | 292,2   1,97  1,12 | 185,7   1,59   0,84 |
| --- | --- | --- | --- |
| Viviane | ng/µl   260/280  260/230 | 324,7   1,96   1,16 | 329,8   1,96   1,16 |
| Jil | ng/µl  260/280   260/230 | 192,4   1,98   1,19 | 263,7   2,01   1,19 |

Nanodrop plasmid

| Fynn | ng/µl   260/280  260/230 | 37,7   1,96  7,29 | 39,1   1,94   5,95 |
| --- | --- | --- | --- |
| Elisa | ng/µl   260/280   260/230 | 84,0   1,93  2,24 | 83,3   1,97   1,92 |

Plasmids DNA-isolation: innuprep plasmid mini kit 2.0
-> protocol 1: step 9: 30µl h2o instead of 50-100µ elution buffer
-> one more time 18.07.

18.07.2018

Nanodrop psb1c3

| Jil | ng/µl   260/280   260/230 | 193,6   1,89   2,84 | 192,1  1,89   1,78 |
| --- | --- | --- | --- |
| Elisa | ng/µl   260/280   260/230 | 33,4   2,00   43,42 | 40,0   2,07   -14,57 |

PCR B.Sub. (bsub\_pelB) 1st try

-PCR Phusion 3 step protocol (without DMSO)
-> fragment size: 1038 bp
-> Tm °C: 62
-> extension: 20s/kb
-Breeding from pz9-plasmids
-Isolated pz9 plasmids+
-> phusion HF buffer: 20µl
-> dNTPs: 5µl
-> fw primer:5µl
-> rv primer: 5µl
-> template DNA: 5µl
-> Phusion polymerase: 1µl
-> h20: 62µl
-> fragment size: ca. 5200 bp
-> Tm°C: 59
-> extension: 2:40

Nanodrop Xan.

| Ben | ng/µl   260/280   260/230 | 109,6   1,99   1,21 | 122,1   1,93   1,23 |
| --- | --- | --- | --- |
| Simon | ng/µl   260/280   260/230 | 109,6   1,96  1,1 | 111,4   1,98   1,15 |

PCR XAN
-1. primer Xan 1+2
-2. primer Xan 3+4
-As breeding pz9
-> fragment size: 745 bp
-> Tm°C: 62
-> 3µl DMSO
-> extension: 30s

PCR B.Sub. 3rd try
-Taq-polymerase
-> long size: 1038 bp
-> Tm°C: 59-70
-> DMS0: 3%

2nd isolation of chromosomal DNA from b.sub. for transformation (standard transformation protocol)
-BBa\_JO4450 Trafo
-Promega standard transformation protocol
-Kit 7, 23 O (iGEM)
-50 µl plated
-Centrifugate: 2 min, 5000rpm
-Pellet resuspended, supernatant plated

20.07.2018

Nanodrop bacillus subtilis

| B.Sub. 1 | ng/µl   260/280   260/230 | 93,7   1,71   0,68 | 90,8   1,81   0,66 |
| --- | --- | --- | --- |
| B.Sub. 2 | ng/µl   260/280   260/230 | 178,2   1,74   0,64 | 176,6   1,72   0,66 |

Nanodrop xanthomonas

| Xan.th> ng/µl   260/280  260/230 | 35,2   1,92   1,76 | 33,1   1,83   1,51 | |
| --- | --- | --- | --- |
| Xan.2 | ng/µl  260/280   260/230 | 40,4   1,84   1,81 | 40,2   1,86   1,80 |

Gelelectrophoresis psb1c3
-1: 1-4 : E, 5-7: J (kept Dna)
-2: 1-3: E, 4-7: J, 8: size standard (new isolated DNA)

PCR Fynn B.sub. 2nd try
->60,8-70,2 °C

24.07.2018

PCR pz9
-MM: 36µl
-H2O: 117µl
-Primer: 9µl
-Template DNA: 9µl
-> gradient PCR: 62,1-68,9 °C

PCR B.Sub. 4th try
-Taq PCR, new DNA
-> approaches : 2x8
-Frag size: 1038 bp
-Tm°C: 60-70
-DMSO: 3%

PCR Xan. Ben&Simon
-> Phusion: protocol
-Frag size: 700, 1200
-Tm°C: 61,5

Plating trafos
-50µl plated
-Sample centrifugated 3min, 4000 rpm
-Supernatant removed
-Resuspend pellet
-Plate

Isolating and PCR: DNA from different trees
-> A: american amber
-> B: oak tree
-> C: hazelnut tree
-> D: maple tree

1.Homogenise samples:
-Liquid nitrogen+ H20: mortar
2.Lyse cellmembrane
-Add 400 µl PL1 -> eppi
+10 µl RNAse, vortex 30 sec.
-Thermoblock: 10min, 65°C
3.Filtrate
-Put nucleosinfilter in collection tube
-Add lysate
-Centrifugate 2min, 11000 rpm
-Flow volume -> eppi
4.Prepare binding:
Add 450µl Pl, vortex 10 sec.
5.Bind DNA
-Put nucleosincolumn in collection tube
-Add 700 µl sample
-Centrifugate 1min., 11000 rpm
-Pour away flow volume
5.1 purify
-Put nucleosincolumn in collection tube
-Add 400µl PW1
-Centrifugate 1min., 11000 rpm
-Pour away flow volume
5.2 purify again
-Nucleosincolumn in collection tube
-Add 700 µl PW2
-Centrifugate 1min., 11000 rpm
-Pour away flow volume
5.3 purify again
-Put nucleosincolumn in collection tube
-Add 200µl PW2
-Centrifugate 2min., 11000 rpm
-Pour away flow volume
6.Eluate
-Nucleosincolumn in sterile eppi
-Add 50 µl PE
-Thermoblock: 5min., 65 °C
-Centrifugate 1min., 11000 rpm
-Pour away nucleosincolumn
-Keep eppi
-> taq PCR

Nanodrop plant DNA

| oak tree | ng/µl   260/280  260/230 | 17,5  1,36  0,58 | 14,2   1,36  0,48 |
| --- | --- | --- | --- |
| hazelnut tree | ng/µl   260/280   260/230 | 12,8   1,35   0,45 | 16,1  1,38   0,75 |
| amber tree | ng/µl   260/230   260/280 | 4,60  1,37   0,54 | 53,6   1,23   0,66 |
| maple tree | ng/µl   260/280   260/230 | 15,88   0,84   0,24 | 10,6   1,08   0,44 |

Gelectrophoresis: did not work-> retry PCR

26.07.2018

New plates 200 mg/ml -> 200 µl/ml (diluted)
-Ampicillin+lb
-e.coli+vector-> plated on new plates
-Plasmids incorporated?

27.07.2018

-LB+CM plates: did not work
-LB+Amp plates: every colony grew
-> 3 clones plated on LB+Amp plates

14.08.2018

PCR plant DNA
Taq PCR: birch, oak, hazelnut, amber, maple
-42µl MM
-24 µl Primermix
-12 µl H2O
-2µl template DNA
-Tm°C: 53

PCR backbones psb1c3 and pz9
-20 µl phusion buffer
-2µl dNTPs
-5µl FW primer
-5µl RV primer
-5µl template DNA
-0,15 µl DMSO
-61,85 µl H2O
-> each

| / | psb1c3 | pz9 |
| --- | --- | --- |
| fragment size | 2070 bp | 5175 bp |
| Tm°c | 62°C | 62°C |
| extensions (s) | 50s | 125s |

-> gelectrophoresis: pz9 (worked) -> Nanodrop, purify, trafo
Psb1c3 ( did not work) -> cut out insert, new

15.08.2018

Purify pz9& insert
-> PCR cleanup: Nucleo spin and PCR Clean-up

Nanodrop pz9 and insert
1.

| pz9 | ng/µl   260/280   260/230 | 2,6   1,34   0,79 | 2,5   0,86   0,59 |
| --- | --- | --- | --- |
| insert | ng/µl   260/280   260/230 | 22,6   1,47   0,45 | 3,0  3,0   0,17 |

2.

| pz9 ng/µl   260/280  260/230 | 2,4   1,56   0,78 | 1,8   1,44   0,79 | | |
| --- | --- | --- | --- | --- |
| insert | ng/µl   260/280   260/230 | 2,8   2,25   0,16 | 2,3   2,28   0,11 |

Pz9: new PCR: 4x50 µl
-50µl hf buffer
-5µl dNTPs
-12,5 µl FW primer
-12,5 µl RV primer
-12,5 µl template DNA
-7,5 µl DMSO
-2,5 µl phusion DNA ploymerase
-147,5 µl H2O
-> worked, multiple seperated bands, purify and cut out

PCR insert XAN:
-36µl phusion hf buffer
-3,6 µl dNTPs
-9µl FW primer
-9µl RV primer
-9µl template DNA
-5,4 µl DMSO
-1,8 µl phusion DNA ploymerase
-106,2 µl H2O
-Fragment size: 5175 bp
-Tm°C: 62
-Extension: 125s
-DMSO: 3%
-> gelectrophoresis: did not work, gradient PCR (60,8-70,8°C)

16.08.2018

Nanodrop purified pz9

| pz9 | ng/µl   260/280   260/230 | 66,5   1,38   0,94 | 65,7   1,37   1,19 |
| --- | --- | --- | --- |

Xan insert: gradient pcr (55-62 °C) , gelectrophoresis: cut out 7&8

DNA from different trees
Retry protocol (14.08.18)
-95,0 °C, 15 min
-95,0 °C 20 sec
-60,0 °C 40 sec
-72,0 °C 35 sec
-72,0 °C 1 min
-32 cycles

17.08.2018

Results of the gelelectophoresis of 16.08.:
-> PCR did not work
Possible new methods:
-New primers
-Synthesis (iGEM)
-Linear PCR (one primer)
-2-step PCR
Linear PCR:
-Like Phusion-PCR, but only with one primer
-A-D fw
-E-H rv
-Gradients: 60,8-70,8 °C
-> Gelelectrophoresis

20.08.2018

PCR of pSB1C3 (2. try):
Phusion-protocol:
-Templates: 9x20µl
-DMSO: 3%
-Frag.-size: 2070 bp
-Tm °C: 56-68 °C
-Extension: 45 s
-> Gelelectrophoresis
Results:
Too many bands: biggest at ca. 3000 bp
-> probably still insert (mCherry) inside the vector
-> possible explanations: primers attached wrong or not at all
-> possible solution: restriction enzymes to cut out the insert before PCR

Gelelectrophoresis on plant-DNA:
Repetition of protocol

21.08.2018

Psb1c3:
Removing mCerry-insert with restriction enzymes:

| prefix | suffix | buffer | Tm°C |
| --- | --- | --- | --- |
| EcoR I (1) | Pst I (1) | O | 37°C |
| Xba I (4) | Pst I (1) | Tango | 37°C |
| Not I | / | O | 37°C |

-4nl DNA (200 µg)
-2 µl Buffer
-1 µl Enzyme
-12 µl water (except for Xbal+Pst I)
-Inactivation: 20 min at 80 °C
-> 2 pieces: 1. pSB1C3 (2070 bp)
2. mCehrry (ca. 711 bp)

Nanodrop results:

| 1st measurement | ng/µl  260/280   260/230 | 81,1   1,83   1,76 |
| --- | --- | --- |
| 2nd measurement | ng/µl   260/280   260/230 | 46,4   1,85   2,01 |
| 3rd measurement | ng/µl   260/280   260/230 | 48,3   1,8   1,38 |
| conclusion | ng/µl   260/280   260/230 | 47,4   1,82   1,5 |

22.08.2018

Gelelectrophoresis did not completely run through the gel, but different bands visable
-> repetition of restriction with Xbal+Pst I
-> gelelectrophoresis in 0.8 % agarose-gel
Results: 2 clear bands
-Colony PCR
-BBa\_K523016 (2085 bp)
-Filling 200 µl of water in eppis and piercing the lid
-Marking 4 colonies on plate and putting them into the eppis
-Cooking eppis in the microwave for 3 minutes
-> PCR with taq-polymerase according to orchid-protocol:
-Fw primer: BBa\_G00100
-Rv primer: BBa\_G00101
-Tm °C: 50 °C
-> Gelelectrophoresis


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# Team:Rheda Bielefeld/Experiments

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TEAM

[Team Members](https://2018.igem.org/Team%3ARheda_Bielefeld/Team)
[Collaborations](https://2018.igem.org/Team%3ARheda_Bielefeld/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ARheda_Bielefeld/Description)
[Design](https://2018.igem.org/Team%3ARheda_Bielefeld/Design)
[Experiments](https://2018.igem.org/Team%3ARheda_Bielefeld/Experiments)
[Notebook](https://2018.igem.org/Team%3ARheda_Bielefeld/Notebook)
[InterLab](https://2018.igem.org/Team%3ARheda_Bielefeld/InterLab)
[Model](https://2018.igem.org/Team%3ARheda_Bielefeld/Model)
[Results](https://2018.igem.org/Team%3ARheda_Bielefeld/Results)
[Demonstrate](https://2018.igem.org/Team%3ARheda_Bielefeld/Demonstrate)
[Improve](https://2018.igem.org/Team%3ARheda_Bielefeld/Improve)
[Attributions](https://2018.igem.org/Team%3ARheda_Bielefeld/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ARheda_Bielefeld/Parts)
[Basic Parts](https://2018.igem.org/Team%3ARheda_Bielefeld/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ARheda_Bielefeld/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ARheda_Bielefeld/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ARheda_Bielefeld/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ARheda_Bielefeld/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ARheda_Bielefeld/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ARheda_Bielefeld/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ARheda_Bielefeld/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ARheda_Bielefeld/Hardware)
[Measurement](https://2018.igem.org/Team%3ARheda_Bielefeld/Measurement)
[Model](https://2018.igem.org/Team%3ARheda_Bielefeld/Model)
[Plant](https://2018.igem.org/Team%3ARheda_Bielefeld/Plant)
[Software](https://2018.igem.org/Team%3ARheda_Bielefeld/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=Rheda_Bielefeld)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

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# Team:Rheda Bielefeld/Results

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TEAM

[Team Members](https://2018.igem.org/Team%3ARheda_Bielefeld/Team)
[Collaborations](https://2018.igem.org/Team%3ARheda_Bielefeld/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ARheda_Bielefeld/Description)
[Design](https://2018.igem.org/Team%3ARheda_Bielefeld/Design)
[Experiments](https://2018.igem.org/Team%3ARheda_Bielefeld/Experiments)
[Notebook](https://2018.igem.org/Team%3ARheda_Bielefeld/Notebook)
[InterLab](https://2018.igem.org/Team%3ARheda_Bielefeld/InterLab)
[Model](https://2018.igem.org/Team%3ARheda_Bielefeld/Model)
[Results](https://2018.igem.org/Team%3ARheda_Bielefeld/Results)
[Demonstrate](https://2018.igem.org/Team%3ARheda_Bielefeld/Demonstrate)
[Improve](https://2018.igem.org/Team%3ARheda_Bielefeld/Improve)
[Attributions](https://2018.igem.org/Team%3ARheda_Bielefeld/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ARheda_Bielefeld/Parts)
[Basic Parts](https://2018.igem.org/Team%3ARheda_Bielefeld/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ARheda_Bielefeld/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ARheda_Bielefeld/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ARheda_Bielefeld/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ARheda_Bielefeld/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ARheda_Bielefeld/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ARheda_Bielefeld/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ARheda_Bielefeld/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ARheda_Bielefeld/Hardware)
[Measurement](https://2018.igem.org/Team%3ARheda_Bielefeld/Measurement)
[Model](https://2018.igem.org/Team%3ARheda_Bielefeld/Model)
[Plant](https://2018.igem.org/Team%3ARheda_Bielefeld/Plant)
[Software](https://2018.igem.org/Team%3ARheda_Bielefeld/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=Rheda_Bielefeld)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)