iGEM 2018 — Team NEU_China_B

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:NEU China B/Notebook

Ruby - Responsive Corporate Tempalte

[go to top](#header)

[![logo.png](https://static.igem.org/mediawiki/2018/thumb/7/70/T--NEU_China_B--led.png/320px-T--NEU_China_B--led.png)](https://2018.igem.org/Team%3ANEU_China_B)
![](https://static.igem.org/mediawiki/2018/6/66/T--NEU_China_B--igem.png )

* Project
  + [Background](https://2018.igem.org/Team%3ANEU_China_B/background)
  + [Description](https://2018.igem.org/Team%3ANEU_China_B/Description)
  + [Applied Design](https://2018.igem.org/Team%3ANEU_China_B/Design)
  + [Demonstration & Result](https://2018.igem.org/Team%3ANEU_China_B/Demonstrate)
  + [Models](https://2018.igem.org/Team%3ANEU_China_B/Model)
* Lab
  + [InterLab](https://2018.igem.org/Team%3ANEU_China_B/InterLab)
  + [Protocols](https://2018.igem.org/Team%3ANEU_China_B/protocols)
  + [Notebook](https://2018.igem.org/Team%3ANEU_China_B/Notebook)
  + [Safety](https://2018.igem.org/Team%3ANEU_China_B/Safety)
* Parts
  + [Basic Parts](https://2018.igem.org/Team%3ANEU_China_B/Basic_Part)
  + [Composite Parts](https://2018.igem.org/Team%3ANEU_China_B/Composite_Part)
  + [Improve](https://2018.igem.org/Team%3ANEU_China_B/Improve)
* Human practice
  + [Human Practices](https://2018.igem.org/Team%3ANEU_China_B/Human_Practices)
  + [Collaborations](https://2018.igem.org/Team%3ANEU_China_B/Collaborations)
* Team
  + [Team Members](https://2018.igem.org/Team%3ANEU_China_B/Team)
  + [Attributions](https://2018.igem.org/Team%3ANEU_China_B/Attributions)

# Notebook

## 2018.1

Team construction, ensure the duties in lab, take a test about security in lab, start to take regular weekly meetings.

## 2018.2

We tried to be familiar with the rules of IGEM. In addition, according to the previous project, we discussed our own project and we decided to plan the project roughly.

## 2018.3

We detailed the plan of our experiment. We choose E. coli K-12 strain, plasmid pET28a and pCDFDuet-1.

## 2018.4

Bought materials, investigation, Genomic extraction

## 2018.5

We tried to extract our genome and PCR the first gene----LsrACDBFG( 6x)

## 2018.6

### 1st week(6/1/2018-6/7/2018)

Extracted genome of E. coli.

 Designed new primer.

 PCR fragment LsrACDBFG with minimal and maximum system respectively.

### 2nd week(6/8/2018-6/14/2018)

PCR target gene LsrACDBFG in three parts

Human practice

Enzyme-cut plasmid pCDFDuet-1 and target fragment GFP

Constructed GFP expression Vectors

### 3rd week(6/15/2018-6/21/2018)

PCR fragment LsrACDBFG with mm system

PCR target fragment LsrACDBFG and plasmid pet-28(a)+

Enzyme-cut target sequence.

Constructed 6x plasmid

Transformed 6x plasmid vectors into pet-28 (a)+ competent cells

Extracted 6x plasmid, enzyme-cut identification to verify the effect of the build

Sequencing

Detection of recombinant strain 6x gene protein expression

### 4th week(6/22/2018-6/28/2018)

PCR target gene LsrACDBFG in three parts

Connecting 6x-1, 6x-2, 6x-3 ( 3 repeated tubes)

Purified fragments

Constructed 6x plasmid

Transformed 6x plasmid vectors into pet-28 (a)+ competent cells

Extracting 6x plasmid from recombinant strains

Enzyme-cut identification to detect build effects

## 2018.7

### 1st week(7/1/2018)

PCR target gene GFP

Constructed plasmid GFP

### 2nd week

PCR target gene LsrACDBFG in three pieces

PCR target gene GFP

### 3rd week

Connected LsrA and GFP

Constructed plasmid 6x + GFP

Transformed 6x plasmid vectors into pet-28 (a)+ competent cells

Extracting 6x + GFP plasmid from recombinant strains

Enzyme-cut identification to detect build effects

### 4th week(7/22/2018-7/28/2018)

PCR plasmid strain 6x + GFP, proved that we construct plasmid 6x + GFP successfully

Detection of recombinant strains 6X+GFP gene expression protein

## 2018.8

### 1st week(8/1/2018-8/7/2018)

Extracted plasmid pet-28(a)+

PCR target fragment Luxs

### 2nd (8/8/2018-8/14/2018)

Extracted total DNA from Escherichia coli

lldR and plasmid pCDFDuet-1 of enzyme-cut target fragments

Constructed plasmid lldR

### 3rd (8/15/2018-8/21/2018)

PCr target fragment LuxS

Constructed plasmid O+LuxS+lldR

Transformed O+LuxS+lldR sequence into DH5-α competent cells

Extraction of O+LuxS+lldR plasmid, enzyme-cut identification to verify the effect of construction

Sequencing

Detection of recombinant strains of protein in the target gene expression

### 4th (8/22/2018-8/28/2018)

PCR target fragment LsrA, GFP

Connected LsrA and GFP

Constructed plasmid LsrA+GFP

PCR target fragment Op

Connected Op and GFP

Constructed plasmid Op+GFP

Constructed plasmid 6x+GFP

## 2018.9

### (9/1/2018-9/15/2018)

Transformed LsrA+GFP, Op+GFP, 6x+GFP plasmid vectors into DH5-α competent cells

Extraction of LsrA+GFP plasmid, OP+GFP plasmid and 6X+GFP plasmid from recombinant strains

Enzyme-cut identification of LsrA+GFP plasmid, OP+GFP plasmid and 6X+GFP plasmid to verify the effect of construction

Sequencing

We combined the various plasmids to confirm the function of the whole system.

Detection of recombinant strain protein expression, verifying the function of constructed parts in recombinant strains

### (9/16/2018-9/30/2018)

We combined the various plasmids to confirm the function of the whole system

Refined and optimized the project parts

### 2018.10

Sent BioBricks, dedicated to the paperwork of the wiki.


[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:NEU China B/Experiments

Ruby - Responsive Corporate Tempalte

[go to top](#header)

[![logo.png](https://static.igem.org/mediawiki/2018/thumb/7/70/T--NEU_China_B--led.png/320px-T--NEU_China_B--led.png)](https://2018.igem.org/Team%3ANEU_China_B)
![](https://static.igem.org/mediawiki/2018/6/66/T--NEU_China_B--igem.png )

* Project
  + [Background](https://2018.igem.org/Team%3ANEU_China_B/background)
  + [Description](https://2018.igem.org/Team%3ANEU_China_B/Description)
  + [Applied Design](https://2018.igem.org/Team%3ANEU_China_B/Design)
  + [Demonstration & Result](https://2018.igem.org/Team%3ANEU_China_B/Demonstrate)
  + [Models](https://2018.igem.org/Team%3ANEU_China_B/Model)
* Lab
  + [InterLab](https://2018.igem.org/Team%3ANEU_China_B/InterLab)
  + [Protocols](https://2018.igem.org/Team%3ANEU_China_B/protocols)
  + [Notebook](https://2018.igem.org/Team%3ANEU_China_B/Notebook)
  + [Safety](https://2018.igem.org/Team%3ANEU_China_B/Safety)
* Parts
  + [Basic Parts](https://2018.igem.org/Team%3ANEU_China_B/Basic_Part)
  + [Composite Parts](https://2018.igem.org/Team%3ANEU_China_B/Composite_Part)
  + [Improve](https://2018.igem.org/Team%3ANEU_China_B/Improve)
* Human practice
  + [Human Practices](https://2018.igem.org/Team%3ANEU_China_B/Human_Practices)
  + [Collaborations](https://2018.igem.org/Team%3ANEU_China_B/Collaborations)
* Team
  + [Team Members](https://2018.igem.org/Team%3ANEU_China_B/Team)
  + [Attributions](https://2018.igem.org/Team%3ANEU_China_B/Attributions)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:NEU China B/Results

Ruby - Responsive Corporate Tempalte

[go to top](#header)

[![logo.png](https://static.igem.org/mediawiki/2018/thumb/7/70/T--NEU_China_B--led.png/320px-T--NEU_China_B--led.png)](https://2018.igem.org/Team%3ANEU_China_B)
![](https://static.igem.org/mediawiki/2018/6/66/T--NEU_China_B--igem.png )

* Project
  + [Background](https://2018.igem.org/Team%3ANEU_China_B/background)
  + [Description](https://2018.igem.org/Team%3ANEU_China_B/Description)
  + [Applied Design](https://2018.igem.org/Team%3ANEU_China_B/Design)
  + [Demonstration & Result](https://2018.igem.org/Team%3ANEU_China_B/Demonstrate)
  + [Models](https://2018.igem.org/Team%3ANEU_China_B/Model)
* Lab
  + [InterLab](https://2018.igem.org/Team%3ANEU_China_B/InterLab)
  + [Protocols](https://2018.igem.org/Team%3ANEU_China_B/protocols)
  + [Notebook](https://2018.igem.org/Team%3ANEU_China_B/Notebook)
  + [Safety](https://2018.igem.org/Team%3ANEU_China_B/Safety)
* Parts
  + [Basic Parts](https://2018.igem.org/Team%3ANEU_China_B/Basic_Part)
  + [Composite Parts](https://2018.igem.org/Team%3ANEU_China_B/Composite_Part)
  + [Improve](https://2018.igem.org/Team%3ANEU_China_B/Improve)
* Human practice
  + [Human Practices](https://2018.igem.org/Team%3ANEU_China_B/Human_Practices)
  + [Collaborations](https://2018.igem.org/Team%3ANEU_China_B/Collaborations)
* Team
  + [Team Members](https://2018.igem.org/Team%3ANEU_China_B/Team)
  + [Attributions](https://2018.igem.org/Team%3ANEU_China_B/Attributions)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)