iGEM 2018 — Team NWU-China

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# Team:NWU-China/Notebook

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[HOME](https://2018.igem.org/Team%3ANWU-China)
[INTERLAB](https://2018.igem.org/Team%3ANWU-China/InterLab)

TEAM

[Team Members](https://2018.igem.org/Team%3ANWU-China/Team)
[Collaborations](https://2018.igem.org/Team%3ANWU-China/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ANWU-China/Description)
[Design](https://2018.igem.org/Team%3ANWU-China/Design)
[Experiments](https://2018.igem.org/Team%3ANWU-China/Experiments)
[Notebook](https://2018.igem.org/Team%3ANWU-China/Notebook)
[Model](https://2018.igem.org/Team%3ANWU-China/Model)
[Results](https://2018.igem.org/Team%3ANWU-China/Results)
[Demonstrate](https://2018.igem.org/Team%3ANWU-China/Demonstrate)
[Improve](https://2018.igem.org/Team%3ANWU-China/Improve)
[Attributions](https://2018.igem.org/Team%3ANWU-China/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ANWU-China/Parts)
[Basic Parts](https://2018.igem.org/Team%3ANWU-China/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ANWU-China/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ANWU-China/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ANWU-China/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ANWU-China/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ANWU-China/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ANWU-China/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ANWU-China/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ANWU-China/Hardware)
[Measurement](https://2018.igem.org/Team%3ANWU-China/Measurement)
[Model](https://2018.igem.org/Team%3ANWU-China/Model)
[Plant](https://2018.igem.org/Team%3ANWU-China/Plant)
[Software](https://2018.igem.org/Team%3ANWU-China/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=NWU-China)

Toggle navigation

[NWU-China](https://2018.igem.org/Team%3ANWU-China)

* [HOME](https://2018.igem.org/Team%3ANWU-China)
* ABOUT US
  + [Team](https://2018.igem.org/Team%3ANWU-China/Team)
  + [Attributions](https://2018.igem.org/Team%3ANWU-China/Attributions)
* PROJECT
  + [Background](https://2018.igem.org/Team%3ANWU-China/Background)
  + [Design](https://2018.igem.org/Team%3ANWU-China/Design)
  + [KcsA Modify](https://2018.igem.org/Team%3ANWU-China/Kcsa%20Modify)
  + [Expand Condon](https://2018.igem.org/Team%3ANWU-China/Expand%20Condon)
  + [Interlab](https://2018.igem.org/Team%3ANWU-China/InterLab)
  + [GROMOSNWU](https://2018.igem.org/Team%3ANWU-China/Future%20Work)
* LAB WORK
  + [Parts](https://2018.igem.org/Team%3ANWU-China/Parts)
  + [Protocols](https://2018.igem.org/Team%3ANWU-China/Protocols)
  + [Notebook](https://2018.igem.org/Team%3ANWU-China/Notebook)
  + [Safety](https://2018.igem.org/Team%3ANWU-China/Safety)
* MODEL
  + [Bacteria](https://2018.igem.org/Team%3ANWU-China/Model)
  + [Protein Modeling](https://2018.igem.org/Team%3ANWU-China/Protein%20Modeling)
* HUMAN PRACTICES
  + [Intergrated](https://2018.igem.org/Team%3ANWU-China/Intergrated)
  + [Public Engagement](https://2018.igem.org/Team%3ANWU-China/Public_Engagement)
  + [Collaborations](https://2018.igem.org/Team%3ANWU-China/Collaborations)
* [AWARDS](https://2018.igem.org/Team%3ANWU-China/Awards)

# Notebook

Week1、2
（2018.4.30—5.13）

·We accepted foundational lab training and learned necessary experiment operation.
·An attempt was made to link the promoter, RBS, Kcsa, terminator to the vector pSB1C3.
·Learn how to make liposomes.

Week3
（2018.5.14—5.20）

·Liposomes were successfully produced and intact liposomes were observed in oil mirrors.

Week4、5
（2018.7.11—7.26）

·Perform Interlab measurements. Communicate interlab data with local teams.
![](https://static.igem.org/mediawiki/2018/4/4e/T--NWU-China--nb.jpg)

Week6、7
（2018.7.30—8.12）

·Consult Dr.Jiang and Prof. Li for advice on improving the experimental protocol.

Week8
（2018.8.13—8.19）

·We designed our target segment according to the standard protocol and handed the BBa\_K2544001;BBa\_K2544002;BBa\_K2544005 to Genscript for synthesis.

Week9
（2018.8.20—8.26）

·The BBa\_K2544001;BBa\_K2544002;BBa\_K2544005 was ligated into the expression vector pET28a and transformed into strain BL21.

Week10、11
（2018.8.27—9.9）

·The protein of the transformed strain was repeatedly extracted, and the reason for the failure of the analysis found that the BBa\_K2544005 design was wrong.
·Redesign BBa\_K2544005 and design improvement part, and hand it over to Genscript.

Week12
（2018.9.10—9.16）

·The BBa\_K2544001;BBa\_K2544002 was ligated into the expression vector pET28a and transformed into the strain DH5α.

Week13
（2018.9.17—9.23）

·The recombinant plasmid was subjected to multiple validations, including agarose gel electrophoresis and colony PCR. But the effect has not been good.
·The BBa\_K2544001;BBa\_K2544002 was ligated to pSB1C3 and transformed into BL21. Verify that the recombinant plasmid still has no good results.
·A control experiment was set up: a stretch of RFP gene was ligated into pET28a and transformed into BL21. Colonies on the medium did not express RFP.

Week14
（2018.9.24—9.30）

·Analyze the operation process and find out the reasons for the failure of the experiment: 1. The restriction enzyme expires. 2. The selection of the enzyme cleavage site on Pet28a is incorrect.
·The BBa\_K2544001;BBa\_K2544002 was recut with a new enzyme, ligated into the vector pSB1C3, and transformed into strain DH5α.

Week15
（2018.10.1—10.7）

·The constructed standardized vector was verified by agarose gel electrophoresis and PCR. Prepare for protein validation.

·A standardized vector for the improved part was constructed, and a control experiment was designed to verify the difference in fluorescence expression efficiency between the part BBa\_K2544006 and the old BBa\_J04430.
·The recombinant plasmid was transformed into BL21.
·The protein was measured and the absorption data of Potassium ions and sodium ions were measured.
![](https://static.igem.org/mediawiki/2018/d/d6/T--NWU-China--nb2.png)
There is a 1000bp marker on the left ,and the second and third channels are KcsA and KcsA\* ，which are about 2700bp.


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# Team:NWU-China/Experiments

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[HOME](https://2018.igem.org/Team%3ANWU-China)
[INTERLAB](https://2018.igem.org/Team%3ANWU-China/InterLab)

TEAM

[Team Members](https://2018.igem.org/Team%3ANWU-China/Team)
[Collaborations](https://2018.igem.org/Team%3ANWU-China/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ANWU-China/Description)
[Design](https://2018.igem.org/Team%3ANWU-China/Design)
[Experiments](https://2018.igem.org/Team%3ANWU-China/Experiments)
[Notebook](https://2018.igem.org/Team%3ANWU-China/Notebook)
[Model](https://2018.igem.org/Team%3ANWU-China/Model)
[Results](https://2018.igem.org/Team%3ANWU-China/Results)
[Demonstrate](https://2018.igem.org/Team%3ANWU-China/Demonstrate)
[Improve](https://2018.igem.org/Team%3ANWU-China/Improve)
[Attributions](https://2018.igem.org/Team%3ANWU-China/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ANWU-China/Parts)
[Basic Parts](https://2018.igem.org/Team%3ANWU-China/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ANWU-China/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ANWU-China/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ANWU-China/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ANWU-China/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ANWU-China/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ANWU-China/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ANWU-China/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ANWU-China/Hardware)
[Measurement](https://2018.igem.org/Team%3ANWU-China/Measurement)
[Model](https://2018.igem.org/Team%3ANWU-China/Model)
[Plant](https://2018.igem.org/Team%3ANWU-China/Plant)
[Software](https://2018.igem.org/Team%3ANWU-China/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=NWU-China)

Toggle navigation

[NWU-China](https://2018.igem.org/Team%3ANWU-China)

* [HOME](https://2018.igem.org/Team%3ANWU-China)
* ABOUT US
  + [Team](https://2018.igem.org/Team%3ANWU-China/Team)
  + [Attributions](https://2018.igem.org/Team%3ANWU-China/Attributions)
* PROJECT
  + [Background](https://2018.igem.org/Team%3ANWU-China/Background)
  + [Design](https://2018.igem.org/Team%3ANWU-China/Design)
  + [KcsA Modify](https://2018.igem.org/Team%3ANWU-China/Kcsa%20Modify)
  + [Expand Condon](https://2018.igem.org/Team%3ANWU-China/Expand%20Condon)
  + [Interlab](https://2018.igem.org/Team%3ANWU-China/InterLab)
  + [GROMOSNWU](https://2018.igem.org/Team%3ANWU-China/Future%20Work)
* LAB WORK
  + [Parts](https://2018.igem.org/Team%3ANWU-China/Parts)
  + [Protocols](https://2018.igem.org/Team%3ANWU-China/Protocols)
  + [Notebook](https://2018.igem.org/Team%3ANWU-China/Notebook)
  + [Safety](https://2018.igem.org/Team%3ANWU-China/Safety)
* MODEL
  + [Bacteria](https://2018.igem.org/Team%3ANWU-China/Model)
  + [Protein Modeling](https://2018.igem.org/Team%3ANWU-China/Protein%20Modeling)
* HUMAN PRACTICES
  + [Intergrated](https://2018.igem.org/Team%3ANWU-China/Intergrated)
  + [Public Engagement](https://2018.igem.org/Team%3ANWU-China/Public_Engagement)
  + [Collaborations](https://2018.igem.org/Team%3ANWU-China/Collaborations)
* [AWARDS](https://2018.igem.org/Team%3ANWU-China/Awards)

# Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

### What should this page contain?

* Protocols
* Experiments
* Documentation of the development of your project

### Inspiration

* [2014 Colombia](https://2014.igem.org/Team%3AColombia/Protocols)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Protocols)
* [2014 Caltech](https://2014.igem.org/Team%3ACaltech/Project/Experiments)

[![](https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg)](https://2018.igem.org)

# Team:NWU-China/Results

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[![](http://placehold.it/350x150/e4dede/c4baba)](https://2018.igem.org/Team%3ANWU-China)
[HOME](https://2018.igem.org/Team%3ANWU-China)
[INTERLAB](https://2018.igem.org/Team%3ANWU-China/InterLab)

TEAM

[Team Members](https://2018.igem.org/Team%3ANWU-China/Team)
[Collaborations](https://2018.igem.org/Team%3ANWU-China/Collaborations)

PROJECT

[Description](https://2018.igem.org/Team%3ANWU-China/Description)
[Design](https://2018.igem.org/Team%3ANWU-China/Design)
[Experiments](https://2018.igem.org/Team%3ANWU-China/Experiments)
[Notebook](https://2018.igem.org/Team%3ANWU-China/Notebook)
[Model](https://2018.igem.org/Team%3ANWU-China/Model)
[Results](https://2018.igem.org/Team%3ANWU-China/Results)
[Demonstrate](https://2018.igem.org/Team%3ANWU-China/Demonstrate)
[Improve](https://2018.igem.org/Team%3ANWU-China/Improve)
[Attributions](https://2018.igem.org/Team%3ANWU-China/Attributions)

PARTS

[Parts Overview](https://2018.igem.org/Team%3ANWU-China/Parts)
[Basic Parts](https://2018.igem.org/Team%3ANWU-China/Basic_Part)
[Composite Parts](https://2018.igem.org/Team%3ANWU-China/Composite_Part)
[Part Collection](https://2018.igem.org/Team%3ANWU-China/Part_Collection)

[SAFETY](https://2018.igem.org/Team%3ANWU-China/Safety)

HUMAN PRACTICES

[Human Practices](https://2018.igem.org/Team%3ANWU-China/Human_Practices)
[Education & Engagement](https://2018.igem.org/Team%3ANWU-China/Public_Engagement)

AWARDS

[Applied Design](https://2018.igem.org/Team%3ANWU-China/Applied_Design)
[Entrepreneurship](https://2018.igem.org/Team%3ANWU-China/Entrepreneurship)
[Hardware](https://2018.igem.org/Team%3ANWU-China/Hardware)
[Measurement](https://2018.igem.org/Team%3ANWU-China/Measurement)
[Model](https://2018.igem.org/Team%3ANWU-China/Model)
[Plant](https://2018.igem.org/Team%3ANWU-China/Plant)
[Software](https://2018.igem.org/Team%3ANWU-China/Software)

[JUDGING FORM ⇗](https://igem.org/2018_Judging_Form?team=NWU-China)

Toggle navigation

[NWU-China](https://2018.igem.org/Team%3ANWU-China)

* [HOME](https://2018.igem.org/Team%3ANWU-China)
* ABOUT US
  + [Team](https://2018.igem.org/Team%3ANWU-China/Team)
  + [Attributions](https://2018.igem.org/Team%3ANWU-China/Attributions)
* PROJECT
  + [Background](https://2018.igem.org/Team%3ANWU-China/Background)
  + [Design](https://2018.igem.org/Team%3ANWU-China/Design)
  + [KcsA Modify](https://2018.igem.org/Team%3ANWU-China/Kcsa%20Modify)
  + [Expand Condon](https://2018.igem.org/Team%3ANWU-China/Expand%20Condon)
  + [Interlab](https://2018.igem.org/Team%3ANWU-China/InterLab)
  + [GROMOSNWU](https://2018.igem.org/Team%3ANWU-China/Future%20Work)
* LAB WORK
  + [Parts](https://2018.igem.org/Team%3ANWU-China/Parts)
  + [Protocols](https://2018.igem.org/Team%3ANWU-China/Protocols)
  + [Notebook](https://2018.igem.org/Team%3ANWU-China/Notebook)
  + [Safety](https://2018.igem.org/Team%3ANWU-China/Safety)
* MODEL
  + [Bacteria](https://2018.igem.org/Team%3ANWU-China/Model)
  + [Protein Modeling](https://2018.igem.org/Team%3ANWU-China/Protein%20Modeling)
* HUMAN PRACTICES
  + [Intergrated](https://2018.igem.org/Team%3ANWU-China/Intergrated)
  + [Public Engagement](https://2018.igem.org/Team%3ANWU-China/Public_Engagement)
  + [Collaborations](https://2018.igem.org/Team%3ANWU-China/Collaborations)
* [AWARDS](https://2018.igem.org/Team%3ANWU-China/Awards)

# Results

Here you can describe the results of your project and your future plans.

### What should this page contain?

* Clearly and objectively describe the results of your work.
* Future plans for the project.
* Considerations for replicating the experiments.

### Describe what your results mean

* Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
* Show data, but remember all measurement and characterization data must be on part pages in the Registry.
* Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

### Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

* A list of linked bullet points of the successful results during your project
* A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

### Inspiration

See how other teams presented their results.

* [2014 TU Darmstadt](https://2014.igem.org/Team%3ATU_Darmstadt/Results/Pathway)
* [2014 Imperial](https://2014.igem.org/Team%3AImperial/Results)
* [2014 Paris Bettencourt](https://2014.igem.org/Team%3AParis_Bettencourt/Results)