Commission Regulation (EC) No 1245/2007 of 24 October 2007 amending Annex I to Regulation (EC) No 2075/2005, as regards the use of liquid pepsin for the detection of Trichinella in meat (Text with EEA relevance)
Date: 2007-10-24
Institutions: European Commission
Subjects: food inspection, food safety, meat processing industry, pigmeat, research method

25.10.2007

EN

Official Journal of the European Union

L 281/19

COMMISSION REGULATION (EC) No 1245/2007

of 24 October 2007

amending Annex I to Regulation (EC) No 2075/2005, as regards the use of liquid pepsin for the
detection of Trichinella in meat

(Text with EEA relevance)

THE COMMISSION OF THE EUROPEAN COMMUNITIES,

(5)

The measures provided in this Regulation are
accordance with
Committee on the Food Chain and Animal Health,

opinion

in
Standing

the

the

of

Having regard to the Treaty
Community,

establishing the European

HAS ADOPTED THIS REGULATION:

Having regard to Regulation (EC) No 854/2004 of the European
Parliament and of the Council of 29 April 2004 laying down
specific rules
the organisation of official controls on
products of animal origin intended for human consumption (1),
and in particular Article 18(9) and (10) thereof,

for

Article 1

Annex I
follows:

to Regulation (EC) No 2075/2005 is amended as

1. Chapter I is amended as follows:

Whereas:

(a) Point 1(p) is replaced by the following:

(1)

(2)

(3)

Commission Regulation (EC) No 2075/2005 of 5
December 2005 laying down specific hygiene rules on
official controls for Trichinella in meat (2) provides for
methods of detection of Trichinella
in samples of
carcases. The reference method laid down in Annex I
to that Regulation requires that
for the detection of
Trichinella larvae in meat samples, 10 ± 0,2 g of pepsin
is to be added to the sample.

‘(p) Pepsin,

strength: 1: 10 000 NF

(US National
Formulary) corresponding to 1: 12 500 BP (British
Pharmacopoeia) and to 2 000 FIP (Fédération inter-
nationale de pharmacie), or stabilized liquid pepsin
with minimum 660 European Pharmacopoeia
units/ml’,

(b) Point 3.I (b) is replaced by the following:

Reports have been published (3)
indicating that pepsin
powder can cause allergic reactions in certain susceptible
individuals.

‘(b) 10 ± 0,2 g of pepsin or 30 ± 0,5 ml liquid pepsin is

added.’

the

sensitivity of

indicated that

Investigations by the Community Reference Laboratory
for Parasites
the
reference method of detection for Trichinella is not
altered when liquid pepsin is used according to the
manufacturer's specifications instead of pepsin powder.
Such an alternative should therefore be provided both
for the reference method and the equivalent method of
detection of Trichinella in meat.

2. Chapter II is amended as follows:

(a) Point A. 1. (q) is replaced by the following:

‘(q) Pepsin,

strength: 1: 10 000 NF

(US National
Formulary) corresponding to 1: 12 500 BP (British
Pharmacopoeia) and to 2 000 FIP (Fédération inter-
nationale de pharmacie), or stabilized liquid pepsin
with minimum 660 European Pharmacopoeia
units/ml’,

(4)

Regulation (EC) No 2075/2005 should therefore be
amended accordingly.

(1) OJ L 139, 30.4.2004, p. 206, as corrected by OJ L 226, 25.6.2004,
p. 83. Regulation as last amended by Council Regulation (EC) No
1791/2006 (OJ L 363, 20.12.2006, p. 1).

(2) OJ L 338, 22.12.2005, p. 60. Regulation as amended by Regulation

(EC) No 1665/2006 (OJ L 320, 18.11.2006, p. 46).
(3) J Investig Allergol Clin Immunol (2006) 16, 136-137.

(b) Point A. 3. II. (a) (v) is replaced by the following:

‘(v) Lastly, 6 g pepsin or 18 ml liquid pepsin is added.
This order must be followed strictly to avoid decom-
position of the pepsin.’,

L 281/20

EN

Official Journal of the European Union

25.10.2007

(c) Point C. 3. I. (h) is replaced by the following:

‘(h) Lastly, add 7 g of pepsin or 21 ml liquid pepsin. This order must be followed strictly to avoid

decomposition of the pepsin.’

This Regulation shall enter into force on the 20th day following that of its publication in the Official Journal
of the European Union.

Article 2

This Regulation shall be binding in its entirety and directly applicable in all Member States.

Done at Brussels, 24 October 2007.

For the Commission

Markos KYPRIANOU
Member of the Commission