Commission Regulation (EC) No 2293/1999 of 14 October 1999 extending the provisional authorisations of certain additives in feedingstuffs (Text with EEA relevance)
Date: 1999-10-14
Institutions: European Commission
Subjects: animal health, animal nutrition, consumer protection, food additive, foodstuffs legislation

6.11.1999

EN

Official Journal of the European Communities

L 284/1

I

(Acts whose publication is obligatory)

COMMISSION REGULATION (EC) No 2293/1999

of 14 October 1999

extending the provisional authorisations of certain additives in feedingstuffs

(Text with EEA relevance)

THE COMMISSION OF THE EUROPEAN COMMUNITIES,

Having regard to the Treaty establishing the European
Community,

Having regard to Council Directive 70/524/EEC of 23
November 1970 concerning additives in feedingstuffs (1), as
(EC) No
Regulation
last
1636/1999 (2), and in particular Article 3, thereof,

Commission

amended

by

(1) Whereas Article 9e(1) of Directive 70/524/EEC provides
that a provisional authorisation of new additives or new
uses of additives may be given, if at the level permitted in
feedingstuffs, it does not adversely affect human or animal
health or the environment, nor harm the consumer by
its
altering the characteristics of
if
presence in feedingstuffs can be controlled, and it
is
reasonable to assume, in view of the available results, that
it has a favourable effect on the characteristics of those
feedingstuffs
production when
or
incorporated in such feedingstuffs;

livestock products,

livestock

on

(2) Whereas Directive 70/524/EEC provides that provisional
authorisations are given as from 1 October 1999 on a
Community level for a period that may not exceed four
years; whereas
additives
provsionally authorised for the first time between 1 April
1998 and 30 September 1999 are subject
to national
expiring on 30 September
provisional authorisations

additives or uses of

the

1999; whereas
provisional authorisations;

it

is appropriate to extend the said

(3) Whereas

for Animal Nutrition
the
authorisation

the Commission has consulted the Scientific
the
Committee
provisional
and
micro-organisms included in the Annex to the present
Regulation, whereas the Committee delivered an opinion
in particular with regard to the safety of these additives;

concerning
enzymes

of

(4) Whereas the measures provided for in this Regulation are
in accordance with the opinion of the Standing Committee
for Feedingstuffs,

HAS ADOPTED THIS REGULATION:

Article 1

Additives referred to in the Annex to this Regulation are
authorised in accordance with Directive 70/524/EEC under the
conditions laid down in this Annex.

Article 2

This Regulation shall enter into force on 1 October 1999.

This Regulation shall be binding in its entirety and directly applicable in all Member States.

Done at Brussels, 14 October 1999.

For the Commission

David BYRNE

Member of the Commission

(1) OJ L 270, 14.12.1970, p. 1.
(2) OJ L 194, 27.7.1999, p. 17.

ANNEX

COLOURING MATTERS INCLUDING PIGMENTS

No
(or EC No)

Additive

Chemical formula, description

Species or
category of animal

Maximum age

Minimum
content

Maximum
content

mg/kg of complete feedingstuff

Other provisions

Period of
authorisation

1. Carotenoids and xanthophylls

E 160a

Beta-carotene

E 161g

Canthaxanthin

C40H56

C40H52O2

Canaries

Pet and
ornamental
birds

(cid:151)

(cid:151)

(cid:151)

(cid:151)

(cid:151)

(cid:151)

(cid:151)

(cid:151)

30.9.2000 (a)

30.9.2000 (a)

12

Astaxanthin-rich Phaffia
rhodozyma (ATCC 74219)

Concentrated biomass of the yeast
Phaffia rhodozyma (ATCC 74219),
least 4,0 g
killed, containing at
astaxanthin
kilogram of
additive and having a maximum
ethoxyquin content of 2 000 mg/kg

per

2. Other colouring matter

Salmon

(cid:151)

(cid:151)

100

The maximum content
astaxanthin

is expressed as

30.9.2000 (a)

Use permitted only from the age of six
months onwards.

of

the

The mixture
additive with
canthaxanthin is allowed provided that the
total
concentration of astaxanthin and
canthaxanthin does not exceed 100 mg/kg
in the complete feedingstuff

Ethoxyquin content to be declared

Trout

(cid:151)

(cid:151)

100

The maximum content
astaxanthin

is expressed as

30.9.2000 (a)

Use permitted only from the age of six
months onwards

L

2
8
4
/
2

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

of

the

The mixture
additive with
canthaxanthin is allowed provided that the
total
concentration of astaxanthin and
canthaxanthin does not exceed 100 mg/kg
in the complete feedingstuff

Ethoxyquin content to be declared

.

.

6
1
1
1
9
9
9

EC No

Element

Additive

Chemical formula

Maximum content of the element in mg/kg of the complete feedingstuff

Other provisions

TRACE ELEMENTS

E 4

Copper-Cu

Copper-lysine sulphate

Cu(C6H13N2O2)2.SO4

Pigs for fattening:

(cid:151) in Member States where the mean density of the porcine
population is equal to or higher than 175 pigs per 100 ha
of utilisable agricultural land:
(cid:151) up to 16 weeks: 175 (total)

(cid:151) in Member States where the mean density of the porcine
population is lower than 175 pigs per 100 ha of utilisable
agricultural land:
(cid:151) up to 16 weeks: 175 (total)

Pigs for fattening:

(cid:151) in Member States where the mean density of the porcine
population is equal to or higher than 175 pigs per 100 ha
of utilisable agricultural land:
(cid:151) from 17th week up to slaughter: 35 (total)

(cid:151) in Member States where the mean density of the porcine
population is lower than 175 pigs per 100 ha of utilisable
agricultural land:
(cid:151) from 17th week up to six months: 100 (total)
(cid:151) over six months up to slaughter: 35 (total)

Breeding pigs: 35 (total)

Other species or categories of animals, with the exception of
calves prior to the start of rumination and sheep: 35 (total)

in

copper

Not more than 50 mg/kg
the
of
feedingstuff
complete
may
from
come
copper-lysine sulphate

in

copper

Not more than 25 mg/kg
the
of
feedingstuffs
complete
may
from
come
copper-lysine sulphate

Period of
authorisation

30.9.2000 (b)

30.9.2000

BINDERS, ANTI-CAKING AGENTS AND COAGULANTS

No
(or EC No)

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

mg/kg of complete feedingstuff

Other provisions

Period of
authorisation

3

Clinoptilolite of volcanic
origin

volcanic

Calcium hydrated aluminosilicate
containing
of
origin
clinoptilolite
minimum 85 % of
and a maximum of 15 % of
feldspar, micas and clays,
free of
fibres and quartz. Maximum lead
content of 80 mg/kg

Pigs

Rabbits

Poultry

(cid:151)

(cid:151)

(cid:151)

(cid:151)

(cid:151)

(cid:151)

20 000

All feedingstuffs

30.9.2000 (c)

20 000

All feedingstuffs

30.9.2000 (c)

20 000

All feedingstuffs

30.9.2000 (c)

.

.

6
1
1
1
9
9
9

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
3

1

2

No

Additive

Chemical formula, description

ENZYMES

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

3-phytase
EC 3.1.3.8

Preparation of 3-phytase produced
by Aspergillus niger
(CBS 114.94)
having a minimum phytase activity
of 5 000 FTU (1)/g for solid and
liquid preparations

Turkeys

(cid:151)

125 FTU

(cid:151)

1.

Indicate in the directions for use for
the additive and the premixture the
storage temperature, storage duration
and stability on pelleting

30.9.2000 (a)

2. Recommended

of
dose
complete feedingstuff: 200 to 800
FTU

per

kg

3-phytase
EC 3.1.3.8

Preparation of 3(cid:150)phytase produced
by Aspergillus oryzae (DSM 10 289)
having a minimum activity of:
Coated form: 2 500 FYT (2)/g
Liquid form: 5 000 FYT/g

Piglets

Four months

250 FYT

1 000 FYT

1.

for use of

the
In the directions
additive and the premixture,
indicate
the storage temperature, storage life,
and stability to pelleting

30.9.2000 (d)

3.

For use in compound feedingstuffs
with a minimum content of 0,3 %
phytate, e.g. 20 % wheat

2. Recommended

dose

per

kg

of

complete feedingstuff: 500 FYT

3.

For use in compound feed rich in
phytates, e.g. containing more than
40 % cereals (corn, barley, oats, wheat,
rye, triticale), oilseeds and pulses

Pigs for
fattening

(cid:151)

400 FYT

1 000 FYT

1.

for use of

the
In the directions
additive and the premixture,
indicate
the storage temperature, storage life,
and stability to pelleting

30.9.2000 (d)

2. Recommended

dose

per

kg

of

complete feedingstuff: 500 FYT

3.

For use in compound feed rich in
phytates, e.g. containing more than
40 % cereals (corn, barley, oats, wheat,
rye, triticale), oilseeds and pulses

L

2
8
4
/
4

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

.

.

6
1
1
1
9
9
9

Chickens for
fattening

(cid:151)

200 FYT

1 000 FYT

1.

for use of

the
In the directions
additive and the premixture,
indicate
the storage temperature, storage life,
and stability to pelleting

30.9.2000 (d)

E
N

2. Recommended

dose

per

kg

of

complete feedingstuff: 500 FYT

3.

For use in compound feed rich in
phytates, e.g. containing more than
40 % cereals (corn, barley, oats, wheat,
rye, triticale), oilseeds and pulses

Laying hens

(cid:151)

500 FYT

1 000 FYT

1.

for use of

the
In the directions
additive and the premixture,
indicate
the storage temperature, storage life,
and stability to pelleting

30.9.2000 (f)

2. Recommended

dose

per

kg

of

complete feedingstuff: 750 FYT

3

Alpha-galactosidase
EC 3.2.1.22

by Aspergillus

Preparation of alpha-galactosidase
oryzae
produced
(DSM 10 286) having a minimum
activity of:
Liquid form: 1 000 GALU (3)/g

Chickens for
fattening

(cid:151)

300 GALU

1 000
GALU

3.

1.

For use in compound feed rich in
phytates, e.g. containing more than
40 % cereals (corn, barley, oats, wheat,
rye, triticale), oilseeds and pulses

for use of

the
In the directions
indicate
additive and the premixture,
the storage temperature, storage life,
and stability on pelleting

30.9.2000 (d)

2. Recommended

kg
complete feedingstuff: 450 GALU

dose

per

of

3.

For use in compound feed rich in
oligosaccharides, e.g. containing more
than 25 % soy meal, cotton seed cakes,
peas

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
5

4

5

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

L

2
8
4
/
6

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

of

Preparation
endo-1,3(4)-beta-
glucanase produced by Aspergillus
(CBS 589.94) having a
aculeatus
minimum activity of:
Coated form: 50 FBG (4)/g
Liquid form: 120 FBG/g

Piglets

Four months

25 FBG

40 FBG

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

E
N

2. Recommended

dose

per

kg

of

complete feedingstuff: 25 FBG

3.

For use in compound feed rich in
non-starch
(mainly
beta-clucans),
containing more
than 50 % corn or barley

polysaccharides

e.g.

Endo-1,4-beta-xylanase
EC 3.2.1.8

of

(DSM 10287) having

Preparation
endo-1,4-beta-
xylanase produced by Aspergillus
oryzae
a
minimum activity of:
Coated form: 1 000 FXU (5)/g
Liquid form: 650 FXU/ml

Chickens for
fattening

(cid:151)

80 FXU

200 FXU

1.

for use of

In the directions
the
indicate the
additive and premixture,
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose

per

kg

of

complete feedingstuff: 150 FXU

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 50 % wheat

polysaccharides

Turkeys for
fattening

(cid:151)

225 FXU

600 FXU

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

2. Recommended

of
dose
complete feedingstuff: 225 to 600 FXU

per

kg

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 50 % wheat

polysaccharides

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

6

Endo-1,4-beta-xylanase
EC 3.2.1.8

Endo-1,4-beta-glucanase
EC 3.2.1.4

of
and

endo-1,4-beta-
Preparation
xylanase
endo-1,4-beta-
glucanase produced by Humicola
insolens
(DSM 10442) having a
minimum activity of:

Coated form:
800 FXU (6)/g
75 FBG (7)/g
Microgranulated form:
800 FXU/g
75 FBG/g
Liquid form:
550 FXU/ml
50 FBG/ml

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Piglets

Four months

200 FXU

(cid:151)

1.

Other provisions

Period of
authorisation

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose

per

kg

of

complete feedingstuff: 200 FXU

3.

For use in compound feed rich in
(mainly
non-starch
arabinoxylans), e.g. containing more
than 50 % wheat

polysaccharides

Chickens for
fattening

(cid:151)

200 FXU
19 FBG

1 000 FXU
94 FBG

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose
complete feedingstuff:
400 FXU
38 FBG

per

kg

of

3.

polysaccharides

For use in compound feed rich in
non-starch
(mainly
arabinoxylans and beta-glucans), e.g.
containing more than 30 % barley
and/or oats, wheat

Piglets

Four months

240 FXU
22 FBG

1 000 FXU
94 FBG

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose
complete feedingstuff:

per

kg

of

400 FXU
38 FBG

3.

polysaccharides

For use in compound feed rich in
non-starch
(mainly
arabinoxylans and beta-glucans), e.g.
containing more than 30 % barley
and/or oats, wheat

.

.

6
1
1
1
9
9
9

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
7

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

L

2
8
4
/
8

Pigs for
fattening

(cid:151)

200 FXU
19 FBG

800 FXU
75 FBG

1.

conditions of use of

the
In the
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (g)

E
N

2. Recommended
feedingstuff:

dose

per

kg

of

400 FXU
38 FBG

3.

polysaccharides

For use in compound feed rich in
(mainly
non-starch
arabinoxylans and beta-glucans), e.g.
containing more than 30 % barley
and/or oats, wheat

Chickens for
fattening

(cid:151)

3 600 FXU

12 000FXU

1.

1 500 BGU

5 000 BGU

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

7

Endo-1,4-beta-xylanase
EC 3.2.1.8

Endo-1,4-beta-glucanase
EC 3.2.1.4

of

and

endo-1,4-beta-
Preparation
xylanase
1,4-beta-
glucanase produced by Aspergillus
niger
a
600.94)
(CBS
minimum activity of:

having

endo

Solid and liquid forms:
12 000 FXU (8)/g
5 000 BGU (9)/g

2. Recommended

dose
complete feedingstuff:

per

kg

of

3 600 to 6 000 FXU
1 500 to 2 500 BGU

3.

polysaccharides

For use in compound feed rich in
non-starch
(mainly
arabinoxylans and beta-glucans), e.g.
containing more than 40 % wheat, rye,
triticale

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

.

.

6
1
1
1
9
9
9

8

Endo-1,4-beta-glucanase
EC 3.2.1.4

Endo-1,4-beta-xylanase
EC 3.2.1.8

of
and

endo-1,4-beta-
Preparation
glucanase
endo-1,4-beta-
xylanase produced by Aspergillus
a
600.94)
(CBS
niger
minimum activity of:

having

Chickens for
fattening

(cid:151)

3 000 BGU

10 000 BGU

1.

1 200 FXU

4 000 FXU

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

E
N

Solid and liquid forms:
10 000 BGU (10)/g
4 000 FXU (11)/g

2. Recommended

dose
complete feedingstuff:

per

kg

of

3 000 to 10 000 BGU
1 200 to 4 000 FXU

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans and arabinoxylans), e.g.
containing more than 30 % barley

polysaccharides

Piglets

Four months

3 000 BGU

5 000 BGU

1.

1 200 FXU

2 000 FXU

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (f)

2. Recommended

dose
complete feedingstuff:

per

kg

of

3 000 to 5 000 BGU
1 200 to 2 000 FXU

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans and arabinoxylans), e.g.
containing more than 30 % barley

polysaccharides

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
9

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

9

Endo-1,4-beta-xylanase
EC 3.2.1.8

10

Alpha-amylase
EC 3.2.1.1

of

Preparation
endo-1,4-beta-
xylanase produced by Aspergillus
a
270.95)
(CBS
niger
minimum activity of:

having

Chickens for
fattening

(cid:151)

1 400 EXU (cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

Solid form: 28 000 EXU (12)/g
Liquid form: 14 000 EXU/ml

2. Recommended

kg
complete feedingstuff: 1 400 EXU

dose

per

of

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 50 % wheat

polysaccharides

Piglets

Four months

1 800 RAU (cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

of

Preparation
produced
amyloliquefaciens
having a minimum activity of:

alpha-amylase
Bacillus
360.94)

(CBS

by

Solid form: 45 000 RAU (13)/g
Liquid form: 20 000 RAU/ml

2. Recommended

kg
complete feedingstuff: 1 800 RAU

dose

per

of

3.

For use, exclusively, in compound feed
liquid feeding systems,
destined for
and
feed
starch-rich
materials (e.g. containing more than
35 % wheat)

containing

Pigs for
fattening

(cid:151)

1 800 RAU (cid:151)

1.

for use of

In the directions
the
indicate the
additive and premixture,
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

kg
complete feedingstuff: 1 800 RAU

dose

per

of

3.

For use, exclusively, in compound feed
liquid feeding systems,
destined for
feed
starch-rich
and
materials (e.g. containing more than
35 % wheat)

containing

L

2
8
4
/
1
0

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

.

.

6
1
1
1
9
9
9

Sows

(cid:151)

1 800 RAU (cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

E
N

2. Recommended

kg
complete feedingstuff: 1 800 RAU

dose

per

of

Chickens
fattening

for

(cid:151)

11

Endo-1,4-beta-glucanase
EC 3.2.1.4

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

Endo-1,4-beta-xylanase
EC 3.2.1.8

of
endo-1,4-beta-
Preparation
glucanase,
endo-1,3(4)-beta-glu-
canase and endo-1,4-beta-xylanase
produced
Trichoderma
by
longibrachiatum (ATCC 74 252)
having a minimum activity of:

Endo-1,4-beta-glucanase:
8 000 U (14)/ml

Endo-1,3(4)-beta-glucanase:
18 000 U (15)/ml

Endo-1,4-beta-xylanase:
26 000 U (16)/ml

Endo-
1,4-beta-
glucanase:
400 U

Endo-
1,3(4)-beta-
glucanase:
900 U

Endo-
1,4-beta-
xylanase:
1 300 U

3.

For use, exclusively, in compound feed
liquid feeding systems,
destined for
and
feed
starch-rich
materials (e.g. containing more than
35 % wheat)

containing

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose
complete feedingstuff:

per

kg

of

endo-1,4-beta-glucanase:
1 600 U

400

to

endo-1,3(4)-beta-glucanase:
3 600 U

900

to

endo-1,4-beta-xylanase:
5 200 U

1 300

to

3.

polysaccharides

For use in compound feed rich in
(mainly
non-starch
arabinoxylans and beta-glucans), e.g.
containing more than 30 % wheat or
barley and 10 % rye

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
1
1

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

12

Endo-1,4-beta-glucanase
EC 3.2.1.4

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

of
endo-1,4-beta-
Preparation
glucanase,
endo-1,3(4)-beta-glu-
canase and endo-1,4-beta-xylanase
viride
produced
(FERM
a
minimum activity of:

Trichoderma

BP-4447)

having

by

Endo-1,4-beta-xylanase
EC 3.2.1.8

Endo-1,4-beta-glucanase:
8 000 U (17)/g

Endo-1,3(4)-beta-glucanase:
18 000 U (18)/g

Endo-1,4-beta-xylanase:
26 000 U (19)/g

Chickens for
fattening

(cid:151)

Endo-
1,4-beta-
glucanase:
200 U

Endo-
1,3(4)-beta-
glucanase:
450 U

Endo-
1,4-beta-
xylanase:
650 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose
complete feedingstuff:

per

kg

of

endo-1,4-beta-glucanase:
1 200 U

800

to

endo-1,3(4)-beta-glucanase: 1 800 to
2 700 U

endo-1,4-beta-xylanase:
3 900 U

2 600

to

3.

polysaccharides

For use in compound feed rich in
non-starch
(mainly
arabinoxylans and beta-glucans), e.g.
containing more than 20 % wheat and
20 % barley, and/or 25 % rye

L

2
8
4
/
1
2

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

.

.

6
1
1
1
9
9
9

Laying hens

(cid:151)

Turkeys for
fattening

(cid:151)

Endo-
1,4-beta-
glucanase:
640 U

Endo-
1,3(4)-beta-
glucanase:
1 440 U

Endo-
1,4-beta-
xylanase:
2 080 U

Endo-
1,4-beta-
glucanase:
1 200 U

Endo-
1,3(4)-beta-
glucanase:
2 700 U

Endo-
1,4-beta-
xylanase:
3 900 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose
complete feedingstuffs:

per

kg

of

endo-1,4-beta-glucanase:
1 280 U

640

to

endo-1,3(4)-beta-glucanase: 1 440 to
2 880 U

endo-1,4-beta-xylanase:
4 160 U

2 080

to

3.

polysaccharides

For use in compound feed rich in
(mainly
non-starch
arabinoxylans and beta-glucans), e.g.
containing more than 20 % wheat and
20 % barley and/or 25 % rye

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose
complete feedingstuffs:

per

kg

of

endo-1,4-beta-glucanase: 1 200 U

endo-1,3(4)-beta-glucanase: 2 700 U

endo-1,4-beta-xylanase: 3 900 U

3.

polysaccharides

For use in compound feed rich in
non-starch
(mainly
arabinoxylans and beta-glucans), e.g.
containing more than 20 % wheat and
20 % barley and/or 20 % rye

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
1
3

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

13

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

Endo-1,4-beta-xylanase
EC 3.2.1.8

14

Endo-1,4-beta-xylanase
EC 3.2.1.8

15

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

of
and

endo-1,3(4)-beta-
Preparation
glucanase
endo-1,4-beta-
xylanase produced by Trichoderma
longibrachiatum
357.94)
having a minimum activity of:

(CBS

Chickens for
fattening

(cid:151)

100 BGU
130 EXU

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

Powder form:
8 000 BGU (20)/g
11 000 EXU (21)/g

Granulated form:
6 000 BGU/g
8 250 EXU/g

Liquid form:
2 000 BGU/ml
2 750 EXU/ml

2. Recommended

dose
complete feedingstuffs:

per

kg

of

100 BGU
130 EXU

3.

polysaccharides

For use in compound feed rich in
non-starch
(mainly
beta-glucans and arabinoxylans), e.g.
containing more than 30 % wheat and
30 % barley or 20 % rye

of

Preparation
endo-1,4-beta-
xylanase produced by Aspergillus
niger
a
520.94)
(CBS
minimum activity of:

having

Solid form: 600 U (22)/g
Liquid form: 300 U/ml

of

Preparation
endo-1,3(4)-beta-
glucanase produced by Trichoderma
viride
a
minimum activity of:

(CBS 517.94)

having

Chickens for
fattening

(cid:151)

300 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose

per

kg

of

complete feedingstuff: 300 to 600 U

3.

For use in compound feed rich in
(mainly
non-starch
arabinoxylans), e.g. containing more
than 50 % wheat

polysaccharides

Chickens for
fattening

(cid:151)

325 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

Solid form: 650 U (23)/g
Liquid form: 325 U/ml

2. Recommended

dose

per

kg

of

complete feedingstuff: 325 to 650 U

3.

For use in compound feed rich in
(mainly
non-starch
beta-glucans),
containing more
than 50 % barley

polysaccharides

e.g.

L

2
8
4
/
1
4

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

.

.

6
1
1
1
9
9
9

16

Endo-1,4-beta-glucanase
EC 3.2.1.4

of

Preparation
endo-1,4-beta-
glucanase produced by Trichoderma
longibrachiatum (IMI
SD 142)
having a minimum activity of:

Solid form: 1 000 CU (24)/g
Liquid form: 2 000 CU/ml

Chickens for
fattening

(cid:151)

250 CU

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

E
N

2. Recommended
complete
1 000 CU

dose
feedingstuff:

per

kg
500

of
to

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans),
containing more
than 40 % barley

polysaccharides

e.g.

Laying hens

(cid:151)

250 CU

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended
complete
1 000 CU

dose
feedingstuff:

per

kg
500

of
to

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans),
containing more
than 40 % barley

polysaccharides

e.g.

Piglets

Four months

250 CU

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended
complete
1 000 CU

dose
feedingstuff:

per

kg
500

of
to

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans),
containing more
than 40 % barley

polysaccharides

e.g.

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
1
5

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

Pigs for
fattening

(cid:151)

250 CU

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended
complete
1 000 CU

dose
feedingstuff:

per

kg
500

of
to

17

Endo-1,4-beta-xylanase

EC 3.2.1.8

of

Preparation
endo-1,4-beta-
xylanase produced by Trichoderma
longibrachiatum (IMI
SD 135)
having a minimum activity of:

Solid form: 3 000 EPU (25)/g
Liquid form: 6 000 EPU/ml

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans),
containing more
than 40 % barley

polysaccharides

e.g.

Chickens for
fattening

(cid:151)

750 EPU

(cid:151)

1.

for use of

the
In the directions
indicate the
additive and premixture,
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended
complete
3 000 EPU

dose

feedingstuff:

per

kg
1 500

of
to

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 40 % wheat or maize

polysaccharides

Laying hens

(cid:151)

750 EPU

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended
complete
3 000 EPU

dose

feedingstuff:

per

kg
1 500

of
to

3.

For use in compound feed rich in
(mainly
non-starch
arabinoxylans), e.g. containing more
than 40 % wheat or maize

polysaccharides

L

2
8
4
/
1
6

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

.

.

6
1
1
1
9
9
9

Piglets

Four months

750 EPU

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

E
N

2. Recommended
complete
3 000 EPU

dose

feedingstuff:

per

kg
1 500

of
to

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 40 % wheat or maize

polysaccharides

Pigs for
fattening

(cid:151)

750 EPU

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended
complete
3 000 EPU

dose

feedingstuff:

per

kg
1 500

of
to

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 40 % wheat or maize

polysaccharides

18

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

of

Preparation
endo-1,3(4)-beta-
glucanase produced by Aspergillus
niger
(MUCL 39199) having a
minimum activity of:

Solid form: 2 000 AGL (26)/g
Liquid form: 500 AGL/ml

Chickens for
fattening

(cid:151)

100 AGL

(cid:151)

1.

for use of

In the directions
the
indicate the
additive and premixture,
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose

per

kg

of

complete feedingstuff: 100 AGL

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans),
containing more
e.g.
than 40 % barley and 20 % wheat

polysaccharides

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
1
7

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

L

2
8
4
/
1
8

19

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

of

Preparation
endo-1,3(4)-beta-
glucanase produced by Aspergillus
(MUCL 39199) having a
niger
minimum activity of:

Solid form: 1 500 AGL (27)/g
Liquid form: 200 AGL/g

Chickens for
fattening

(cid:151)

25 AGL

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

E
N

2. Recommended

of
dose
complete feedingstuff: 25 to 100 AGL

per

kg

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans),
containing more
than 50 % barley

polysaccharides

e.g.

20

Endo-1,4-beta-xylanase
EC 3.2.1.8

of

Preparation
endo-1,4-beta-
xylanase produced by Trichoderma
longibrachiatum (MUCL
39203)
having a minimum activity of:

Solid form: 2 000 AXC (28)/g
Liquid form: 500 AXC/ml

Chickens for
fattening

(cid:151)

100 AXC

(cid:151)

1.

for use of

In the directions
the
indicate the
additive and premixture,
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose

per

kg

of

complete feedingstuff: 100 AXC

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 40 % wheat or rye

polysaccharides

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

21

Endo-1,4-beta-xylanase
EC 3.2.1.8

of

endo-1,4-beta-
Preparation
xylanase produced by Trichoderma
longibrachiatum (MUCL
39203)
having a minimum activity of:

Solid form: 1 500 AXC (29)/g
Liquid form: 200 AXC/g

Chickens for
fattening

(cid:151)

25 AXC

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

of
dose
complete feedingstuff: 25 to 100 AXC

per

kg

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 50 % wheat

polysaccharides

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

.

.

6
1
1
1
9
9
9

22

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

of

Preparation
endo-1,3(4)-beta-
glucanase produced by Trichoderma
longibrachiatum (CNCM MA 6-10
W) having a minimum activity of:

Solid form: 70 000 BGN (30)/g
Liquid form: 14 000 BGN/ml

23

Endo-1,4-beta-xylanase
EC 3.2.1.8

of

Preparation
endo-1,4-beta-
xylanase produced by Trichoderma
longibrachiatum (CNCM MA 6-10
W) having a minimum activity of:

Solid form: 70 000 IFP (31)/g
Liquid form: 7 000 IFP/ml

24

Endo-1,4-beta-xylanase
EC 3.2.1.8

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

of

and

endo-1,4-beta-
Preparation
xylanase
endo-1,3(4)-beta-
glucanase produced by Aspergillus
niger
(CNCM I-1517) having a
minimum activity of:

28 000 QXU (32)/g
140 000 QGU (33)/g

Chickens for
fattening

(cid:151)

1 050 BGN (cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

E
N

2. Recommended

kg
complete feedingstuff: 2 800 BGN

dose

per

of

3.

For use in compound feed rich in
(mainly
non-starch
beta-glucans),
containing more
than 50 % barley

polysaccharides

e.g.

Chickens for
fattening

(cid:151)

1 050 IFP (cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose

per

kg

of

complete feedingstuff: 1 400 IFP

3.

For use in compound feed rich in
(mainly
non-starch
arabinoxylans), e.g. containing more
than 56 % wheat

polysaccharides

Chickens for
fattening

(cid:151)

420 QXU

1 120 QXU

1.

2 100 QGU

5 600 QGU

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose
complete feedingstuffs:

per

kg

of

560 QXU
2 800 QGU

3.

polysaccharides

For use in compound feed rich in
non-starch
(mainly
arabinoxylans and beta-glucans), e.g.
containing more than 30 % wheat and
30 % barley

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
1
9

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

25

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

Endo-1,4-beta-xylanase
EC 3.2.1.8

of
and

endo-1,3(4)-beta-
Preparation
glucanase
endo-1,4-beta-
xylanase produced by Aspergillus
niger
a
minimum activity of:

(NRRL 25541) having

Endo-1,3(4)-beta-glucanase:
1 100 U (34)/g

Endo-1,4-beta-xylanase:
1 600 U (35)/g

Chickens for
fattening

(cid:151)

Laying hens

(cid:151)

Endo-
1,3(4)-beta-
glucanase:
138 U

Endo-
1,4-beta-
xylanase:
200 U

Endo-
1,3(4)-beta-
glucanase:
138 U

Endo-
1,4-beta-
xylanase:
200 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose
complete feedingstuffs:

per

kg

of

endo-1,3(4)-beta-glucanase:
138 U
endo-1,4-beta-xylanase:
200 U

3.

polysaccharides

For use in compound feed rich in
non-starch
(mainly
beta-glucans and arabinoxylans), e.g.
containing more than 50 % barley or
30 % wheat and 30 % corn

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (d)

2. Recommended

dose
complete feedingstuffs:

per

kg

of

endo-1,3(4)-beta-glucanase:
138 U
endo-1,4-beta-xylanase:
200 U

3.

polysaccharides

For use in compound feed rich in
non-starch
(mainly
arabinoxylans and beta-glucans), e.g.
containing more than 50 % barley or
30 % wheat and 30 % corn

L

2
8
4
/
2
0

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

26

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

27

Endo-1,4-beta-xylanase
EC 3.2.1.8

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

Chickens for
fattening

(cid:151)

23 000 BU (cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (e)

2. Recommended
complete
50 000 BU

dose

feedingstuff:

per

kg
23 000

of
to

of

Preparation
endo-1,3(4)-beta-
glucanase produced by Trichoderma
reesei
having
(CBS
minimum activity of:

526.94)

Solid form: 350 000 BU (36)/g
Liquid form: 50 000 BU/g

3.

For use in compound feed rich in
non-starch
(mainly
glucans), e.g. containing more than
20 % barley or 30 % rye

polysaccharides

Piglets

Four months

26 000 BU (cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (e)

2. Recommended
complete
35 000 BU

dose

feedingstuff:

per

kg
26 000

of
to

of

(CBS 529.94)

Preparation
endo-1,4-beta-
xylanase produced by Trichoderma
reesei
and endo-
1,3(4)-beta-glucanase produced by
(CBS 526.94)
reesei
Trichoderma
having a minimum activity of:

Solid form:
200 000 BXU (37)/g
200 000 BU (38)/g

Liquid form:
30 000 BXU/g
30 000 BU/g

3.

For use in compound feed rich in
non-starch
(mainly
glucans), e.g. containing more than
60 % barley or wheat

polysaccharides

Chickens for
fattening

(cid:151)

2 500 BXU

(cid:151)

1.

2 500 BU

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (e)

2. Recommended

dose
complete feedingstuff:

per

kg

of

10 000 BXU
10 000 BU

3.

polysaccharides

For use in compound feed rich in
(mainly
non-starch
arabinoxylans
e.g.
and
containing more than 40 % wheat or
30 % rye

glucans),

.

.

6
1
1
1
9
9
9

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
2
1

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

28

3-phytase
EC 3.1.3.8

Preparation of 3-phytase produced
by Trichoderma reesei (CBS 528.94)
having a minimum activity of:

Solid form: 5 000 PPU (39)/g
Liquid form: 1 000 PPU/g

Piglets

Four months

250 PPU

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (e)

2. Recommended

of
dose
complete feedingstuff: 500 to 750
PPU

per

kg

3.

For use in compound feed rich in
phytates, e.g. containing more than
50 % cereals
(corn, barley, wheat),
tapioca, oilseeds and pulses

Pigs for
fattening

(cid:151)

500 PPU

(cid:151)

1.

for use of

In the directions
the
indicate the
additive and premixture,
storage temperature, storage life, and
stability to pelleting

30.9.2000 (e)

2. Recommended

of
dose
complete feedingstuff: 500 to 750
PPU

per

kg

29

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

of

Preparation
endo-1,3(4)-beta-
glucanase produced by Geosmithia
emersonii
(IMI SD 133) having a
minimum activity of:

Chickens for
fattening

5 500 U (40)/g

3.

For use in compound feed rich in
phytates, e.g. containing more than
(corn, barley, wheat),
50 % cereals
tapioca, oilseeds and pulses

(cid:151)

250 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (f)

2. Recommended

per
complete feedingstuff: 250 U

dose

kg

of

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans), e.g. containing more
than 50 % barley

polysaccharides

L

2
8
4
/
2
2

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

30

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

Endo-1,4-beta-xylanase
EC 3.2.1.8

of
and

endo-1,3(4)-beta-
Preparation
glucanase
endo-1,4-beta-
xylanase produced by Penicillium
funiculosum (IMI SD 101) having a
minimum activity of:

Chickens for
fattening

(cid:151)

Powder form:

Endo-1,3(4)-beta-glucanase:
2 000 U (41)/g
Endo-1,4-beta-xylanase:
1 400 U (42)/g

Liquid form:

Endo-1,3(4)-beta-glucanase:
500 U/ml
Endo-1,4-beta-xylanase:
350 U/ml

of

endo-1,4-beta-
Preparation
xylanase produced by Trichoderma
longibrachiatum
614.94)
having a minimum activity of:

(CBS

Solid form: 300 EU (43)/g
Liquid form: 1 000 EU/g

31

Endo-1,4-beta-xylanase
EC 3.2.1.8

Endo-
1,3(4)-beta-
glucanase:
100 U
Endo-
1,4-beta-
xylanase:
70 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (f)

2. Recommended

per

kg

of

dose
complete feedingstuffs:
endo-1,3(4)-beta-glucanase:
100 U
endo-1,4-beta-xylanase:
70 U

3.

polysaccharides

For use in compound feed rich in
non-starch
(mainly
beta-glucans and arabinoxylans), e.g.
containing more than 50 % barley or
60 % wheat

Chickens for
fattening

(cid:151)

600 EU

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (f)

2. Recommended

dose

per

kg

of

complete feedingstuff: 600 EU

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 60 % wheat

polysaccharides

Laying hens

(cid:151)

300 EU

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (f)

2. Recommended

dose

per

kg

of

complete feedingstuff: 600 EU

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 60 % wheat

polysaccharides

.

.

6
1
1
1
9
9
9

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
2
3

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

L

2
8
4
/
2
4

32

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

of

Preparation
endo-1,3(4)-beta-
glucanase produced by Trichoderma
2106)
longibrachiatum
having a minimum activity of:

(ATCC

Chickens for
fattening

(cid:151)

100 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (g)

E
N

200 U (44)/ml

2. Recommended

per
complete feedingstuff: 100 U

dose

kg

of

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans), e.g. containing more
than 30 % barley

polysaccharides

1 200 U/ml

Piglets

Four months

400 U

(cid:151)

1.

for use of

In the directions
the
indicate the
additive and premixture,
storage temperature, storage life, and
stability to pelleting

30.9.2000 (g)

2. Recommended

per
complete feedingstuff: 400 U

dose

kg

of

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans), e.g. containing more
than 55 % barley

polysaccharides

Pigs for
fattening

(cid:151)

500 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (g)

2. Recommended

per
complete feedingstuff: 500 U

dose

kg

of

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans), e.g. containing more
than 70 % barley

polysaccharides

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

.

.

6
1
1
1
9
9
9

33

Endo-1,4-beta-xylanase
EC 3.2.1.8

of

Preparation
endo-1,4-beta-
xylanase produced by Trichoderma
longibrachiatum
2105)
having a minimum activity of:

(ATCC

Chickens for
fattening

(cid:151)

500 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (g)

E
N

Powder form: 2 000 U (45)/g

Liquid form: 5 000 U/ml

2. Recommended

of
dose
complete feedingstuff: 500 to 2 500 U

per

kg

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 55 % wheat or 60 % rye

polysaccharides

Laying hens

(cid:151)

2 000 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (g)

2. Recommended

dose

per

kg

of

complete feedingstuff: 2 000 U

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 35 % wheat

polysaccharides

Powder form: 4 000 U/g

Piglets

Four months

5 000 U

(cid:151)

1.

Liquid form: 10 000 U/ml

for use of

the
In the directions
indicate the
additive and premixture,
storage temperature, storage life, and
stability to pelleting

30.9.2000 (g)

2. Recommended

dose

per

kg

of

complete feedingstuff: 5 000 U

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 45 % wheat

polysaccharides

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
2
5

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

Powder form: 4 000 U/g

Liquid form: 8 000 U/ml

Pigs for
fattening

(cid:151)

4 000 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (g)

2. Recommended

dose

per

kg

of

complete feedingstuff: 4 000 U

3.

For use in compound feed rich in
non-starch
(mainly
arabinoxylans), e.g. containing more
than 35 % wheat

polysaccharides

(cid:151)

1.

for use of

the
In the directions
indicate the
additive and premixture,
storage temperature, storage life, and
the stability to pelleting

30.9.2000 (h)

2. Recommended

dose
complete feedingstuffs:

per

kg

of

endo-1,3(4)-beta-glucanase: 165 U
endo-1,4-beta-xylanase: 240 U
alpha-amylase: 1 860 U

3.

For use in compound feed containing
cereals rich in starch and non-starch
polysaccharides (mainly arabinoxylans
and
containing
more than 45 % barley and 10 %
wheat or 10 % maize

beta-glucans),

e.g.

Piglets

Four months

Endo-
1,3(4)-beta-
glucanase:
165 U

Endo-
1,4-beta-
xylanase:
240 U

Alpha-amy-
lase:
1 860 U

34

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

Endo-1,4-beta-xylanase
EC 3.2.1.8

Alpha-amylase
EC 3.2.1.1

of
and

endo-1,3(4)-beta-
Preparation
endo-1,4-beta-
glucanase
xylanase produced by Aspergillus
of
niger
(NRRL
produced
alpha-amylase
by
Aspergillus oryzae
(ATCC 66222)
having a minimum activity of:

25541)

and

Endo-1,3(4)-beta-glucanase:
275 U (46)/g

Endo-1,4-beta-xylanase:
400 U (47)/g

Alpha-amylase:
3 100 U (48)/g

L

2
8
4
/
2
6

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

.

.

6
1
1
1
9
9
9

35

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

Endo-1,4-beta-xylanase
EC 3.2.1.8

Laying hens

(cid:151)

of

Preparation
endo-1,3(4)-beta-
glucanase produced by Trichoderma
longibrachiatum (ATCC 2106) and
produced
endo-1,4-beta-xylanase
by
longibrachiatum
Trichoderma
(ATCC 2105) having a minimum
activity of:

endo-1,3(4)-beta-glucanase:
80 U (49)/g
endo-1,4-beta-xylanase: 180 U (50)/g

36

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

Endo-1,4-beta-xylanase
EC 3.2.1.8

Chickens for
fattening

(cid:151)

of

Preparation
endo-1,3(4)-beta-
glucanase produced by Trichoderma
longibrachiatum (ATCC 2106) and
endo-1,4-beta-xylanase
produced
by Trichoderma longibrachiatum (IMI
SD 135)
a minimum
activity of:

having

endo-1,3(4)-beta-glucanase:
300 U (51)/g

endo-1,4-beta-xylanase: 300 U (52)/g

Endo-
1,3(4)-beta-
glucanase:
80 U
Endo-
1,4-beta-
xylanase:
180 U

Endo-
1,3(4)-beta-
glucanase:
300 U

Endo-
1,4-beta-
xylanase:
300 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (h)

E
N

2. Recommended

dose
complete feedingstuffs:

per

kg

of

endo-1,3(4)-beta-glucanase: 80 U
endo-1,4-beta-xylanase: 180 U

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans and arabinoxylans), e.g.
containing more than 60 % barley

polysaccharides

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (h)

2. Recommended

dose
complete feedingstuffs:

per

kg

of

endo-1,3(4)-beta-glucanase: 300 U
endo-1,4-beta-xylanase: 300 U

3.

For use in compound feed rich in
non-starch
(mainly
beta-glucans and arabinoxylans), e.g.
containing more than 40 % barley

polysaccharides

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
2
7

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

Laying hens

(cid:151)

Endo-
1,3(4)-beta-
glucanase:
300 U
Endo-
1,4-beta-
xylanase:
300 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (h)

37

Endo-1,4-beta-xylanase
EC 3.2.1.8

Subtilisin
EC 3.4.21.62

of

Preparation
endo-1,4-beta-
xylanase produced by Trichoderma
longibrachiatum (ATCC 2105) and
subtilisin
by Bacillus
subtilis
(ATCC 2107) having a
minimum activity of:

produced

endo-1,4-beta-xylanase:
2 500 U (53)/g
subtilisin: 800 U (54)/g

Chickens for
fattening

(cid:151)

Turkeys

(cid:151)

(cid:151)

(cid:151)

Endo-
1,4-beta-
xylanase:
500 U
Subtilisin:
160 U

Endo-
1,4-beta-
xylanase:
825 U
Subtilisin:
265 U

L

2
8
4
/
2
8

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

.

.

6
1
1
1
9
9
9

2. Recommended

dose
complete feedingstuffs:

per

kg

of

endo-1,3(4)-beta-glucanase: 300 U
endo-1,4-beta-xylanase: 300 U

3.

1.

For use in compound feed rich in
non-starch
(mainly
beta-glucans and arabinoxylans), e.g.
containing more than 35 % barley

polysaccharides

for use of

the
In the directions
indicate the
additive and premixture,
storage temperature, storage life, and
stability to pelleting

30.9.2000 (h)

2. Recommended

dose
complete feedingstuff:

per

kg

of

endo-1,4-beta-xylanase:
2 500 U
subtilisin: 160 to 800 U

500

to

3.

1.

For use
containing more than 65 % wheat

in compound feed e.g.

for use of

In the directions
the
indicate the
additive and premixture,
storage temperature, storage life, and
stability to pelleting

30.9.2000 (h)

2. Recommended

dose
complete feedingstuff:

per

kg

of

endo-1,4-beta-xylanase:
2 500 U
subtilisin: 265 to 800 U

825

to

3.

For use
containing more than 45 % wheat

in compound feed e.g.

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

Period of
authorisation

38

Endo-1,4-beta-xylanase
EC 3.2.1.8

Subtilisin
EC 3.4.21.62

of

Preparation
endo-1,4-beta-
xylanase produced by Trichoderma
longibrachiatum (ATCC 2105) and
subtilisin
by Bacillus
(ATCC 2107) having a
subtilis
minimum activity of:

produced

Piglets

Four months

Endo-
1,4-beta-
xylanase:
5 000 U
Subtilisin:
500 U

endo-1,4-beta-xylanase:
5 000 U (55)/g
subtilisin: 500 U (56)/g

39

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

Endo-1,4-beta-xylanase
EC 3.2.1.8

Pigs for
fattening

(cid:151)

of

endo-1,3(4)-beta-
Preparation
glucanase produced by Trichoderma
longibrachiatum (ATCC 2106) and
produced
endo-1,4-beta-xylanase
by
longibrachiatum
Trichoderma
(ATCC 2105) having a minimum
activity of:

endo-1,3(4)-beta-glucanase:
400 U (57)/g
endo-1,4-beta-xylanase: 400 U (58)/g

Chickens for
fattening

(cid:151)

40

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

Endo-1,4-beta-xylanase
EC 3.2.1.8

Subtilisin
EC 3.4.21.62

of

(ATCC

Preparation
endo-1,3(4)-beta-
glucanase produced by Trichoderma
longibrachiatum
2106),
produced
endo-1,4-beta-xylanase
longibrachiatum
Trichoderma
by
(ATCC
subtilisin
2105)
produced by Bacillus subtilis (ATCC
2107) having a minimum activity
of:

and

endo-1,3(4)-beta-glucanase:
100 U (59)/g
endo-1,4-beta-xylanase: 300 U (60)/g
subtilisin: 800 U (61)/g

(cid:151)

(cid:151)

Endo-
1,3(4)-beta-
glucanase:
400 U
Endo-
1,4-beta-
xylanase:
400 U

Endo-
1,3(4)-beta-
glucanase:
30 U
Endo-
1,4-beta-
xylanase:
90 U
Subtilisin:
240 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (h)

2. Recommended

dose
complete feedingstuff:

per

kg

of

endo-1,4-beta-xylanase: 5 000 U
subtilisin: 500 U

3.

1.

For use
containing more than 40 % wheat

in compound feed e.g.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (h)

2. Recommended

dose
complete feedingstuffs:

per

kg

of

endo-1,3(4)-beta-glucanase: 400 U
endo-1,4-beta-xylanase: 400 U

3.

1.

For use in compound feed rich in
non-starch
(mainly
beta-glucans and arabinoxylans), e.g.
containing more than 65 % barley

polysaccharides

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (h)

2. Recommended

dose
complete feedingstuffs:

per

kg

of

endo-1,3(4)-beta-glucanase:
100 U
endo-1,4-beta-xylanase: 90 to 300 U
subtilisin: 240 to 800 U

30

to

3.

For use
containing more than 60 % barley

in compound feed e.g.

.

.

6
1
1
1
9
9
9

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
2
9

No

Additive

Chemical formula, description

Species or
category of
animal

Maximum age

Minimum
content

Maximum
content

Units of activity per kg of
complete feedingstuff

Other provisions

41

Endo-1,3(4)-beta-glucanase
EC 3.2.1.6

Endo-1,4-beta-xylanase
EC 3.2.1.8

Subtilisin
EC 3.4.21.62

of

(ATCC

Preparation
endo-1,3(4)-beta-
glucanase produced by Trichoderma
longibrachiatum
2106),
produced
endo-1,4-beta-xylanase
longibrachiatum
Trichoderma
by
(ATCC
subtilisin
2105)
produced by Bacillus subtilis (ATCC
2107) having a minimum activity
of:

and

endo-1,3(4)-beta-glucanase:
100 U (62)/g
endo-1,4-beta-xylanase:
2 500 U (63)/g
subtilisin: 800 U (64)/g

Chickens for
fattening

(cid:151)

Laying hens

(cid:151)

Period of
authorisation

30.9.2000 (h)

Endo-
1,3(4)-beta-
glucanase:
25 U
Endo-
1,4-beta-
xylanase:
625 U
Subtilisin:
200 U

(cid:151)

1.

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

2. Recommended

dose
complete feedingstuffs:

per

kg

of

endo-1,3(4)-beta-glucanase:
100 U
endo-1,4-beta-xylanase:
2 500 U
subtilisin: 200 to 800 U

25

625

to

to

(cid:151)

Endo-
1,3(4)-beta-
glucanase:
100 U

Endo-
1,4-beta-
xylanase:
2 500 U

Subtilisin:
800 U

3.

1.

in compound feed e.g.
For use
containing more than 30 % wheat and
10 % barley

for use of

In the directions
the
indicate the
additive and premixture,
storage temperature, storage life, and
stability to pelleting

30.9.2000 (h)

2. Recommended

dose
complete feedingstuffs:

per

kg

of

endo-1,3(4)-beta-glucanase: 100 U
endo-1,4-beta-xylanase: 2 500 U
subtilisin: 800 U

3.

1.

in compound feed e.g.
For use
containing more than 50 % wheat and
25 % barley

for use of

the
In the directions
additive and premixture,
indicate the
storage temperature, storage life, and
stability to pelleting

30.9.2000 (h)

2. Recommended

dose

per

kg

of

complete feedingstuff: 4 000 U

3.

For use in compound feed rich in
(mainly
non-starch
arabinoxylans), e.g. containing more
than 60 % wheat

polysaccharides

L

2
8
4
/
3
0

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

.

.

6
1
1
1
9
9
9

Piglets

Four months

4 000 U

(cid:151)

42

Endo-1,4-beta-xylanase
EC 3.2.1.8

of

endo-1,4-beta-
Preparation
xylanase produced by Trichoderma
longibrachiatum (IMI
SD 135)
having a minimum activity of:

Solid form: 4 000 U (65)/g

Characteristics of
preparation:

the authorised

endo-1,4-beta-xylanase: 1,99 %

wheat: 97,7 %

calcium propionate: 0,3 %

lecithin: 0,01 %

No

Additive

Chemical formula, description

MICRO-ORGANISMS

Species or
category of
animal

Minimum
content

Maximum
content

Maximum age

Other provisions

CFU/kg of complete
feedingstuff

1

Bacillus cereus var. toyoi

NCIMB 40112/CNCM
I-1012

Preparation of Bacillus
cereus var.
toyoi containing a minimum of
1 (cid:215) 1010 CFU/g additive

Chickens for
fattening

(cid:151)

0,2 (cid:215) 109

1 (cid:215) 109

Laying hens

(cid:151)

0,2 (cid:215) 109

1 (cid:215) 109

Calves

Six months

0,5 (cid:215) 109

1 (cid:215) 109

Cattle for
fattening

(cid:151)

0,2 (cid:215) 109

0,2 (cid:215) 109

In the directions for use of the additive
storage
and premixture,
temperature, storage life and stability to
pelleting

indicate

the

permitted

lasolacid sodium,

May be used in compound feed containing
coccidiostats: monensin
the
sodium,
salinomycin
amprolium-ethopabate,
sodium,
benzoquate,
meticlorpindol-methyl
decoquinate,
narasin,
halofuginone

robenidine,

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

The quantity of Bacillus cereus var. toyoi in
the daily ration must not exceed 1,0 (cid:215) 109
CFU for 100 kg body weight. Add
0,2 (cid:215) 109 CFU for each additional 100 kg
body weight.

.

.

6
1
1
1
9
9
9

Period of
authorisation

30.9.2000 (g)

30.9.2000 (g)

30.9.2000 (g)

30.9.2000 (g)

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
3
1

No

Additive

Chemical formula, description

Species or
category of
animal

Minimum
content

Maximum
content

Maximum age

Other provisions

CFU/kg of complete
feedingstuff

Breeding does

(cid:151)

0,1 (cid:215) 109

5 (cid:215) 109

Rabbits for
fattening

(cid:151)

0,1 (cid:215) 109

5 (cid:215) 109

3

Saccharomyces cerevisiae

NCYC Sc 47

Saccharomyces
of
Preparation
cerevisiae containing a minimum of
5 (cid:215) 109 CFU/g additive

Rabbits for
fattening

(cid:151)

2,5 (cid:215) 109

5 (cid:215) 109

Sows

(cid:151)

5 (cid:215) 109

2,5 (cid:215) 1010

Piglets

Four months

5 (cid:215) 109

1 (cid:215) 1010

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

May be used in compound feed containing
the permitted coccidiostats: robenidine

In the directions for use of the additive
storage
and premixture,
temperature, storage life and stability to
pelleting

indicate

the

May be used in compound feed containing
the permitted coccidiostats: meticlorpindol,
robenidine, salinomycin sodium

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

May be used in compound feed containing
the permitted coccidiostat: meticlorpindol

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

L

2
8
4
/
3
2

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

Period of
authorisation

30.9.2000 (g)

30.9.2000 (g)

30.9.2000 (d)

30.9.2000 (d)

30.9.2000 (d)

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

Species or
category of
animal

Minimum
content

Maximum
content

Maximum age

Other provisions

CFU/kg of complete
feedingstuff

4

Bacillus cereus
ATCC 14 893

Preparation
containing
1010 CFU/g additive

of
a

Bacillus
minimum

Piglets

cereus
of

Four months

5 (cid:215) 108

1 (cid:215) 1010

Pigs for
fattening

Sows

(cid:151)

2 (cid:215) 108

1 (cid:215) 109

8,5 (cid:215) 108

1,2 (cid:215) 109

15 days before
farrowing and
during
lactation

Calves

16 weeks

1 (cid:215) 109

1,2 (cid:215) 109

Chickens for
fattening

(cid:151)

2 (cid:215) 108

1 (cid:215) 109

Turkeys for
fattening

26 weeks

2 (cid:215) 108

1 (cid:215) 109

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
storage
and premixture,
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

May be used in compound feed containing
the permitted coccidiostats: amprolium,
sodium,
halofuginone,
monensin
maduramicin
sodium,
sodium,
meticlorpindol, diclazuril

lasalocid
ammonium,

salinomycin

narasin,

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

May be used in compound feed containing
the permitted coccidiostats: amprolium,
halofuginone, meticlorpindol/methylbenzo-
quate, diclazuril, nifursol

Period of
authorisation

30.9.2000 (d)

30.9.2000 (d)

30.9.2000 (d)

30.9.2000 (d)

30.9.2000 (d)

30.9.2000 (d)

.

.

6
1
1
1
9
9
9

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
3
3

No

Additive

Chemical formula, description

Species or
category of
animal

Minimum
content

Maximum
content

Maximum age

Other provisions

CFU/kg of complete
feedingstuff

5

Saccharomyces cerevisiae
CBS 493.94

Preparation
Saccharomyces
of
cerevisiae containing a minimum of
1 (cid:215) 108 CFU/g additive

Calves

Six months

2 (cid:215) 108

2 (cid:215) 109

Cattle for
fattening

(cid:151)

1,7 (cid:215) 108

1,7 (cid:215) 108

6

Saccharomyces cerevisiae

CNCM I-1079

Preparation
Saccharomyces
of
cerevisiae containing a minimum of
2 (cid:215) 1010 CFU/g additive

Sows

(cid:151)

2 (cid:215) 109

1 (cid:215) 1010

Piglets

Four months

6 (cid:215) 109

3 (cid:215) 1010

7

Saccharomyces cerevisiae

CNCM I-1077

Preparation
Saccharomyces
of
cerevisiae containing a minimum of
2 (cid:215) 1010 CFU/g additive

Dairy cows

(cid:151)

5,5 (cid:215) 108

2,1 (cid:215) 109

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

daily

The quantity of Saccharomyces cerevisiae in
the
exceed
ration must
7,5 (cid:215) 108 CFU for 100 kg body weight.
Add 1 (cid:215) 108 CFU for each extra 100 kg
body weight

not

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

daily

The quantity of Saccharomyces cerevisiae in
the
exceed
ration must
8,4 (cid:215) 109 CFU for 100 kg body weight.
Add 1,8 (cid:215) 109 CFU for each additional
100 kg body weight

not

L

2
8
4
/
3
4

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

Period of
authorisation

30.9.2000 (d)

30.9.2000 (f)

30.9.2000 (d)

30.9.2000 (d)

30.9.2000 (d)

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

Species or
category of
animal

Minimum
content

Maximum
content

Maximum age

Other provisions

CFU/kg of complete
feedingstuff

Period of
authorisation

.

.

6
1
1
1
9
9
9

8

9

Enterococcus faecium
ATCC 53519

Enterococcus faecium
ATCC 55593

(In a 1/1 ratio)

Pediococcus acidilactici
CNCM MA 18/5M

of

encapsulated

encapsulated
Mixture
faecium ATCC 53519
Enterococcus
and
Enterococcus
faecium ATCC 55593 containing a
minimum of 2 (cid:215) 108 CFU/g of the
additive
a minimum of
1 (cid:215) 108 CFU/g of each bacterium)

(i.e.

Preparation of Pediococcus acidilactici
of
containing
1 (cid:215) 1010 CFU/g of additive

minimum

a

Cattle for
fattening

(cid:151)

1 (cid:215) 109

1,5 (cid:215) 109

Chickens for
fattening

(cid:151)

1 (cid:215) 108

1 (cid:215) 108

Chickens for
fattening

(cid:151)

1 (cid:215) 109

1 (cid:215) 1010

Piglets

Four months

1 (cid:215) 109

1 (cid:215) 109

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

daily

The quantity of Saccharomyces cerevisiae in
the
exceed
ration must
4,6 (cid:215) 109 CFU for 100 kg body weight.
Add 2 (cid:215) 109 CFU for
each additional
100 kg body weight.

not

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

May be used in compound feed containing
the permitted coccidiostats: amprolium,
lasalocid
decoquinate,
sodium,
ammonium,
monensin sodium, narasin, nicarbazin,
narasin/nicarbazin, salinomycin sodium

halofuginone,

maduramicin

In the directions for use of the additive
storage
and premixture,
temperature, storage life and stability to
pelleting

indicate

the

May be used in compound feed containing
the permitted coccidiostats: amprolium,
meticlorpindol, decoquinate, halofuginone,
narasin, salinomycin sodium, nicarbazin,
maduramicin ammonium, diclazuril

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

30.9.2000 (d)

E
N

30.9.2000 (d)

30.9.2000 (f)

30.9.2000 (f)

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
3
5

No

Additive

Chemical formula, description

Species or
category of
animal

Minimum
content

Maximum
content

Maximum age

Other provisions

CFU/kg of complete
feedingstuff

Pigs

(cid:151)

1 (cid:215) 109

1 (cid:215) 109

10

Enterococcus faecium
NCIMB 10415

Preparation of Enterococcus faecium
containing a minimum of

Chickens for
fattening

(cid:151)

0,3 (cid:215) 109

2,8 (cid:215) 109

Microencapsulated form::
1,0 (cid:215) 1010 CFU/g additive
1,75 (cid:215) 1010 CFU/g additive

Pigs

(cid:151)

0,35 (cid:215) 109

1,5 (cid:215) 109

Sows

(cid:151)

0,2 (cid:215) 109

1,25 (cid:215) 109

Cattle for
fattening

(cid:151)

0,25 (cid:215) 109

0,6 (cid:215) 109

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

May be used in compound feed containing
the permitted coccidiostats: amprolium,
amprolium/ethopabate,
diclazuril,
halofuginone, maduramicin ammonium,
meticlorpindol, meticlorpindol/methylben-
robenidine,
zoquate, monensin sodium,
salinomycin sodium

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
storage
and premixture,
temperature, storage life and stability to
pelleting

indicate

the

The quantity of Enterococcus faecium in the
daily ration must not exceed 1 (cid:215) 109 CFU
for 100 kg body weight. Add 1 (cid:215) 109 CFU
for each additional 100 kg body weight

L

2
8
4
/
3
6

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

Period of
authorisation

30.9.2000 (f)

30.9.2000 (f)

30.9.2000 (f)

30.9.2000 (f)

30.9.2000 (f)

.

.

6
1
1
1
9
9
9

No

Additive

Chemical formula, description

Species or
category of
animal

Minimum
content

Maximum
content

Maximum age

Other provisions

CFU/kg of complete
feedingstuff

Period of
authorisation

.

.

6
1
1
1
9
9
9

Preparation of Enterococcus faecium
containing a minimum of:

Piglets

Four months

0,3 (cid:215) 109

1,4 (cid:215) 109

Microencapsulated form:
1,0 (cid:215) 1010 CFU/g additive
1,75 (cid:215) 1010 CFU/g additive

and

Granulated form:
3,5 (cid:215) 1010 CFU/g additive

Calves

Six months

0,35 (cid:215) 109

6,6 (cid:215) 109

11

Enterococcus faecium
DSM 5464

Preparation of Enterococcus faecium
a minimum of:
containing
5 (cid:215) 1010 CFU/g additive

Piglets

Four months

0,5 (cid:215) 109

1 (cid:215) 109

12

Lactobacillus farciminis

CNCM MA 67/4R

Preparation
Lactobacillus
of
farciminis containing a minimum
of: 1 (cid:215) 109 CFU/g additive

Piglets

Four months

1 (cid:215) 109

1 (cid:215) 1010

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

Granulated form to be used exclusively in
milk replacers

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

Granulated form to be used exclusively in
milk replacers

In the directions for use of the additive
storage
and premixture,
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

30.9.2000 (f)

E
N

30.9.2000 (f)

30.9.2000 (f)

30.9.2000 (g)

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
3
7

No

Additive

Chemical formula, description

Species or
category of
animal

Minimum
content

Maximum
content

Maximum age

Other provisions

CFU/kg of complete
feedingstuff

13

Enterococcus faecium

DSM 10 663/
NCIMB 10 415

Preparation of Enterococcus faecium
containing a minimum of:

Piglets

Four months

1 (cid:215) 109

1 (cid:215) 1010

Powder and granulated forms:
3,5 (cid:215) 1010 CFU/g additive

Coated form:
2,0 (cid:215) 1010 CFU/g additive

Liquid form:
1 (cid:215) 1010 CFU/g additive

Calves

Six months

1 (cid:215) 109

1 (cid:215) 1010

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

Period of
authorisation

30.9.2000 (g)

30.9.2000 (h)

Chickens for
fattening

(cid:151)

1 (cid:215) 109

1 (cid:215) 1010

1.

for use of

the
In the directions
indicate the
additive and premixture,
storage temperature, storage life and
stability to pelleting

30.9.2000 (h)

2. May be used in compound feed
containing the permitted coccidiostats:
amprolium,
amprolium ethopabat,
decoquinate, diclazuril, halofuginone,
lasalocid
maduramicin
ammonium, meticlorpindol/methyl-
sodium,
benzoquate,
robenidine,
narasin,
salinomycin sodium

nicarbazin,

monensin

sodium,

14

Saccharomyces cerevisiae
MUCL 39 885

Preparation
Saccharomyces
of
cerevisiae containing a minimum of:

Piglets

Four months

3 (cid:215) 109

3 (cid:215) 109

spheric

Powder,
granulated forms:
1 (cid:215) 109 CFU/g additive

and

oval

Cattle for
fattening

(cid:151)

9 (cid:215) 109

9 (cid:215) 109

In the directions for use of the additive
storage
and premixture,
temperature, storage life and stability to
pelleting

indicate

the

In the directions for use of the additive
and premixture,
storage
temperature, storage life and stability to
pelleting

indicate

the

30.9.2000 (g)

30.9.2000 (g)

L

2
8
4
/
3
8

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

daily

The quantity of Saccharomyces cerevisiae in
the
exceed
ration must
1,6 (cid:215) 1010 CFU per 100 kg body weight.
Add 3,2 (cid:215) 109 CFU for each additional
100 kg body weight

not

.

.

6
1
1
1
9
9
9

First authorisation Commission Regulation (EC) No 2316/98 (OJ L 289, 28.10.1998, p. 4).

First authorisation Commission Regulation (EC) No 1245/1999 (OJ L 150, 17.6.1999, p. 15).

(a)
(b) First authorisation Commission Regulation (EC) No 639/1999 (OJ L 82, 26.3.1999, p. 6).
(c)
(d) First authorisation Commission Regulation (EC) No 1436/98 (OJ L 191, 7.7.1998, p. 15).
(e)
First authorisation Commission Regulation (EC) No 2374/98 (OJ L 295, 4.11.1998, p. 3).
(f)
First authorisation Commission Regulation (EC) No 866/1999 (OJ L 108, 27.4.1999, p. 21).
(g) First authorisation Commission Regulation (EC) No 1411/1999 (OJ L 164, 30.6.1999, p. 56).
(h) First authorisation Commission Regulation (EC) No 1636/1999 (OJ L 194, 27.7.1999, p. 17).
(1) FTU is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute from sodium phytate at pH 5,5 and 37 (cid:176)C.
(2) FYT is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute from sodium phytate at pH 5,5 and 37 (cid:176)C.
(3) 1 GALU is the amount of enzyme which hydrolyses 1 micromole of p-nitrophenyl-alpha-galactopyranoside per minute at pH 5,0 and 30 (cid:176)C.
(4) 1 FBG is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,5 and 37 (cid:176)C.
(5) 1 FXU is the amount of enzyme which liberates 7,8 micromoles of reducing sugars ( xylose equivalents) from azo-wheat arabinoxylan per minute at pH 6,0 and 50 (cid:176)C.
(6) 1 FXU is the amount of enzyme which liberates 3,1 micromoles of reducing sugars (xylose equivalents) from azo-wheat arabinoxylan per minute at pH 6,0 and 50 (cid:176)C.
(7) 1 FBG is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 6,0 and 50 (cid:176)C.
(8) 1 FXU is the amount of enzyme which liberates 0,15 micromoles of xylose from azurine-cross-linked xylan per minute at pH 5,0 and 40 (cid:176)C.
(9) 1 BGU is the amount of enzyme which liberates 0,15 micromoles of glucose from azurine-cross-linked beta-glucan per minute at pH 5,0 and 40 (cid:176)C.
(10) 1 BGU is the amount of enzyme which liberates 0,15 micromoles of glucose from azurine-cross-linked beta-glucan per minute at pH 5,0 and 40 (cid:176)C.
(11) 1 FXU is the amount of enzyme which liberates 0,15 micromoles of xylose from azurine-cross-linked-xylan per minute at pH 5,0 and 40 (cid:176)C.
(12) 1 EXU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from arabinoxylan per minute at pH 3,5 and 55 (cid:176)C.
(13) 1 RAU is the amount of enzyme which converts 1 mg of soluble starch into a product having an equal absorption to a reference colour at 620 nm after reaction with iodine, per minute at pH 6,6 and 30 (cid:176)C.
(14) 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from carboxymethylcellulose per minute at pH 5,0 and 40 (cid:176)C.
(15) 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from barley beta-glucan per minute at pH 5,0 and 40 (cid:176)C.
(16) 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from oat spelt xylan per minute at pH 5,0 and 40 (cid:176)C.
(17) 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from carboxymethylcellulose per minute at pH 5,0 and 40 (cid:176)C.
(18) 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from barley beta-glucan per minute at pH 5,0 and 40 (cid:176)C.
(19) 1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from oat spelt xylan per minute at pH 5,0 and 40 (cid:176)C.
(20) 1 BGU is the amount of enzyme which liberates 0,278 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 3,5 and 40 (cid:176)C.
(21) 1 EXU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from wheat arabinoxylan per minute at pH 3,5 and 55 (cid:176)C.
(22) 1 U is the amount of enzyme which liberates 1 micromole of xylose from birchwood xylan per minute at pH 5,3 and 50 (cid:176)C.
(23) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 (cid:176)C.
(24) 1 CU is the amount of enzyme which liberates 0,128 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,5 and 30 (cid:176)C.
(25) 1 EPU is the amount of enzyme which liberates 0,0083 micromoles of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 4,7 and 30 (cid:176)C.
(26) 1 AGL is the amount of enzyme which liberates 5,55 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 4,6 and 30 (cid:176)C.
(27) 1 AGL is the amount of enzyme which liberates 5,55 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 4,6 and 30 (cid:176)C.
(28) 1 AXC is the amount of enzyme which liberates 17,2 micromoles of reducing sugars (maltose equivalents) from oat xylan per minute at pH 4,7 and 30 (cid:176)C.
(29) 1 AXC is the amount of enzyme which liberates 17,2 micromoles reducing sugars (maltose equivalents) from oat xylan per minute at pH 4,7 and 30 (cid:176)C.
(30) 1 BGN is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,8 and 50 (cid:176)C-
(31) 1 IFP is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat xylan per minute at pH 4,8 and 50 (cid:176)C.
(32) 1 QXU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat xylan per minute at pH 5,1 and 50 (cid:176)C.
(33) 1 QGU is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,8 and 50 (cid:176)C.
(34) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from oat beta-glucan per minute at pH 4,0 and 30 (cid:176)C.
(35) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat xylan per minute at pH 4,0 and 30 (cid:176)C.
(36) 1 BU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,8 and 50 (cid:176)C.
(37) 1 BXU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (xylose equivalents) from birch xylan per minute at pH 5,3 and 50 (cid:176)C.
(38) 1 BU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,8 and 50 (cid:176)C.
(39) 1 PPU is the amount of enzyme which liberates 1 micromole of inorganic phosphate from sodium phytate per minute at pH 5,0 at 37 (cid:176)C.
(40) 1 U is the amount of enzyme which liberates 2,78 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 5,0 and 50 (cid:176)C.
(41) 1 U is the amount of enzyme which liberates 5,55 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 5,0 and 50 (cid:176)C.
(42) 1 U is the amount of enzyme which liberates 4,00 micromoles of reducing sugars (maltose equivalents) from birchwood xylan per minute at pH 5,5 and 50 (cid:176)C.
(43) 1 EU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat xylan per minute at pH 4,5 and 40 (cid:176)C.
(44) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 (cid:176)C.
(45) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 (cid:176)C.
(46) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from oat beta-glucan per minute at pH 4,0 and 30 (cid:176)C.
(47) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from oat xylan per minute at pH 4,0 and 30 (cid:176)C.
(48) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from wheat starch per minute at pH 4,0 and 30 (cid:176)C.
(49) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 (cid:176)C.
(50) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 (cid:176)C.
(51) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 (cid:176)C.
(52) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 (cid:176)C.
(53) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 (cid:176)C.
(54) 1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 (cid:176)C.
(55) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) oat spelt xylan per minute at pH 5,3 and 50 (cid:176)C.

.

.

6
1
1
1
9
9
9

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

L

2
8
4
/
3
9

(56) 1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 (cid:176)C.
(57) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 (cid:176)C.
(58) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 (cid:176)C.
(59) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 (cid:176)C.
(60) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 (cid:176)C.
(61) 1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 (cid:176)C.
(62) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 (cid:176)C.
(63) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 (cid:176)C.
(64) 1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 (cid:176)C.
(65) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 (cid:176)C.

L

2
8
4
/
4
0

E
N

O

f
f
i
c
i
a
l

J
o
u
r
n
a
l

o
f

t
h
e

E
u
r
o
p
e
a
n

C
o
m
m
u
n
i
t
i
e
s

.

.

6
1
1
1
9
9
9