 "En Ami" first aired in the United States on March 19, 2000. This episode earned a Nielsen rating of 7.5, with an 11 share, meaning that roughly 7.5 percent of all television-equipped households, and 11 percent of households watching television, were tuned in to the episode. It was viewed by 11.99 million viewers. The episode aired in the United Kingdom and Ireland on Sky1 on June 25, 2000 and received 0.62 million viewers, ranking as the fourth most watched episode that week. Fox promoted the episode with a faux-cigarette ad that read "Warning: Tonight's episode contains the Cigarette Smoking Man and may be harmful to Agent Scully's health." The episode was later included on The X-Files Mythology, Volume 3 – Colonization, a DVD collection that contains episodes involved with the alien Colonist's plans to take over the earth.
