 The cast of FOX musical series Glee performed this song on the episode "Blame It on the Alcohol", with Heather Morris'character, Brittany Pierce, taking the lead. The episode revolved around teen drinking and its dangers. The members of Glee Club are asked to perform at the school's alcohol awareness assembly, in which "Tik Tok" is one of the songs performed. Todd VanDerWerff of The A.V. Club wrote that the song's inclusion in the episode was superfluous, stating that it was just an excuse to get a Kesha song on Glee. VanDerWerff however, wrote that he "REALLY liked Heather Morris'" rendition of the song. Sandra Gonzalez of Entertainment Weekly praised Brittany's choreography and overall performance in "Tik Tok", writing, "The huge star of this number was clearly Brittany, who more and more every week proves that she needs to be moved to the forefront of this show's big performances and storylines." Gonzalez gave the cover version of "Tik Tok" a B, calling it "pure, fun entertainment up until we got to the part straight out of the mind of Gordie LaChance. So even though the purple vomit was a bit too much for this weak viewer, the performance made me add a Ke $ ha song to my iTunes, which I never thought would happen." Erica Futterman of Rolling Stone gave the cover version of "Tik Tok" a mostly positive review, writing, "Love Brittany as we do, we wish Rachel or Mercedes stepped up to the mic. The performance is less risqué than their Pep Rally" Push It "but winds up causing more controversy when Brittany pukes on Rachel and Santana also vomits up grey slush. It's a fitting end to the song, and the episode."
