 Potter's source for the wildlife and the insect drawings in Mrs. Tittlemouse were those she had executed in her early adulthood, either directly from nature or by observing specimens in the collections of the Victoria and Albert Museum. The interest in the book's illustrations lies in the microscopic accuracy of the insects rather than in any human qualities exhibited by Mrs. Tittlemouse or Mr. Jackson. Potter is uncharacteristically careless in the depiction of the insects however. They appear to be drawn for their own sake, or seem to be out of scale with the heroine, or to change scale without reason. The ladybird seems larger than Mrs. Tittlemouse, and the spider appears first larger than Mrs. Tittlemouse in one picture and then smaller in another. The bees are sometimes out of scale with both the toad and the mouse.
