 Mary Jane was voiced by Vanessa Marshall. Marshall has voice acted on several animated series and video games, as well as starring in commercials and other on-screen roles and performing stand-up comedy. Marshall was filming a Honda commercial when her agent, Cathey Lizzio, called her to confirm that she had landed the role as Mary Jane, simply saying, "Hello, is this Mary Jane?" Overjoyed, Marshall dropped to her knees and began hyperventilating, which worried the producers of the commercial. Marshall's mother had voiced Spider-Woman in the original 1980s Spider-Man animated series, though Marshall's interest in Spider-Man had dated back to reading the comic books in her youth. On her character, Marshall noted that she is "incredibly complex:"
