 William IV, Duke of Bavaria commissioned The Battle of Alexander at Issus in 1528. Altdorfer was approximately 50 at the time, and was living in the Free Imperial City of Regensburg. As a result of over a decade of involvement with the Regensburg city council, Altdorfer was offered the position of Burgomaster on 18 September 1528. He declined; the council annals reported his reasoning as such: "He much desires to execute a special work in Bavaria for my Serene Highness and gracious Lord, Duke [William]." William probably wanted the painting for his newly built summer Lusthaus ("pleasure house") in the grounds of his palace in Munich, approximately 60 miles (97 km) south of Regensburg. There, it was to hang alongside seven other paintings with a similar format and subject matter, including Ludwig Refinger's The Matyrdom of Marcus Curtius, Melchior Feselen's The Siege of Alesia by Caesar, and the painting of Battle of Cannae by Hans Burgkmair (1473 – 1531). Another eight, each portraying a famous woman from history, were later added to the set, probably at the behest of the Duke's wife, Jacobaea of Baden. Altdorfer's Susanna and the Elders (1526) was among these.
