The cycling parameters were as follows: One cycle of denaturation, annealing and extension were carried out at 94°C for 1 min , 62°C for 10 sec , 72°C for 10 sec respectively.
40 cycles of denaturation, annealing and extension were carried out at 94°C for 10 sec , 94°C for 10 sec , 94°C for 10 sec respectively.