[ 0182] The resulting BSA-loaded calcium carbonate microspheres were incubated with dithiothreitol (DTT) solutions with concentrations ranging from 0.01 to 1 mM at pH 7.5 for 15-60 minutes at room temperature.
The BSA-loaded calcium carbonate microspheres were washed five times with buffer (pH 7.4) by centrifugation (2000 rpm , 2 minutes) and redispersion cycles.