Immunoblotting of proteins isolated from transiently transfected cells expressing chimera Sstr5 [TM5-6Sstr3mut2] confirmed that the mutant chimeric receptor was expressed (Supplementary Figure 1).
The A and Q residues in chimeric receptor Htr7 [TM5-V241Htr6] were mutated to phenylalanine and ciliary localization was then quantified in IMCD cells expressing Htr6, Htr7, Htr7 [TM5-V241Htr6], or Htr7 [TM5-V241Htr6mut].