At the end of the incubation, the cells were washed twice with PBS and suspended in 200 μl of PBS containing 1% fetal bovine serum (FBS), and subjected to FACS analyses.
Sp35-COS-7 cells or Sp35-CHO-cells were mixed with mouse serum or hybridoma supernatant and then treated with R-phycoerythrin-conjugated goat anti-mouse secondary antibody and directly subjected to standard FACS analyses.