in contrast, t7‐hpacv and t7‐hpacgv significantly reduced the transactivation from the hre promoter under hypoxia (fig 3c), indicating a marked loss of endogenous hif function.
T7‐HPACGV was reproducibly more potent in attenuating HIF‐mediated transcription than T7‐HPACV (Fig 3C), and thus the T7‐HPACGV chimaera was selected for subsequent experimentation.