To determine if ICAM-2 is a constituent of the apical ectoplasmic specialization (ES), co-immunoprecipitation (co-IP) and dual immunofluorescence staining were performed.
ICAM-2 was found to associate with β1-integrin, nectin-3, afadin, Src, proline-rich tyrosine kinase 2 (Pyk2), annexin II and actin.